LOCUS       DAOPUR010000001        10210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_6630_length_10210_cov_3.411521, whole genome shotgun sequence.
ACCESSION   DAOPUR010000001 DAOPUR010000000
VERSION     DAOPUR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6630_length_10210_cov_3.411521"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2719
                     /gene="rpoB"
                     /locus_tag="PKN90_00005"
     CDS             <1..2719
                     /gene="rpoB"
                     /locus_tag="PKN90_00005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNZ84699.1"
                     /translation="INKKLDLDISPEIKVLTKEDIIAIIRYLIELINSKADVDDIDHL
                     SNRRVRTVGEQLCNQFSVGLARMARTIRERMNVRDNEVFTPIDLINAKAISSVINSFF
                     GTNALSQFMDQTNPLAEMTHKRRMSALGPGGLSRERAGFEVRDVHYTHYGRLCPIETP
                     EGPNIGLISSLCVYAKINDLGFIETPYRKVEDKKVNLNKEDIIYLSAEEEENIIIAQG
                     NAPLDDNGNFIRTRVKARQDSDFPIVAPEEVQLMDVSPTQIASIAASLIPFLEHDDAN
                     RALMGSNMMRQAVPLLRCEAPIVGTGLEGQIIKDSRTQITAERDGVIEYVDATTIKVR
                     YNYSEEELFVQFESSVKEYTLPKFQKTNQSTTIDLRPIVHKGEKVVAGQILTEGYSTQ
                     QGELAIGKNLKVAFMPWKGYNFEDAIVINERLVREDVFTSIHVDEYSLEVRETKRGLE
                     ELTADIPNVSEDATKNLDENGIVRIGALIEPGDILIGKITPKGESDPSPEEKLLRAIF
                     GDKAGDVKDASLKAHPSLRGVVIEKRLFSKAQKAKKSKSADKAIMDKIEAEFEEKAHA
                     LKLKLVEKLLVLTNGKTSQGVKDFLGAEVISKGSKFTQKQLLDVDYSTVQISKWTSDS
                     AKNEQIKTLIMNYLKKYKEYDAQLKREKYNITIGDELPAGIIQLAKVYIAKKRKVQVG
                     DKMAGRHGNKGIVSRIVRQEDMPFLEDGSPVDIVLNPLGVPSRMNLGQIFETVLGWAG
                     KELNVKFSTPIFDGATLEDLDEWTTKANIPRYGKTYLYDGGTGEKFDQPATVGVIYML
                     KLGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAFGAAHILQEIL
                     TVKSDDVMGRAKTYEAIVKGDPMPAPGIPESLNVLLHELRGLGLSINLE"
     gene            2784..7073
                     /gene="rpoC"
                     /locus_tag="PKN90_00010"
     CDS             2784..7073
                     /gene="rpoC"
                     /locus_tag="PKN90_00010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNZ84700.1"
                     /translation="MAFRKENKAKSSFSKISIGLASPEEILEHSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTDKKVRRERMGHIQ
                     LVVPVAHIWYFRSLPNKIGYLLGISTKKLDSIIYYERYVVIQAGTQSDLEVGDLLTED
                     EYLNIMDALPRENYLLEDTDPNKFIAKMGAEAIYDMLSRLDLDKISYELRDKASNDTS
                     QQRKTEALKRLQVVEAFRASKQRNKPEWMVVKVVPVIPPELRPLVPLDGGRFATSDLN
                     DLYRRVIIRNNRLKKLIEIKAPDVILRNEKRMLQEAVDSLFDNTRKSTAVKADANRPL
                     KSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKPFVI
                     RKLIERGIVKTVKSAKKIVDRKDPVVWDILEHVMKGHPVLLNRAPTLHRLGIQAFQPK
                     MIEGKAIQLHPLSCTAFNADFDGDQMAVHLPLGNEAVLEAQILMLASHNILNPANGAP
                     ITVPSQDMVLGLYYMTKGRSGALGEGLKFYSPKEVIIAYNERKVSLHANISVKTQDVE
                     NGVIVTKMIETTVGRVILNEYVPKKVGYINEILSKKSLRDIIGKIINVCGIADTANFL
                     DDIKSLGYQMAFKGCLSFNLDNIIIPKEKEELVQQGYAEVEEILANYNMGFITYNERY
                     NQIIDTWTHINSKLSNILMKQLSTDDQGFNSVYMMLDSGARGSKEQIRQLSGMRGLMA
                     KPQKSGAEGGQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTRRL
                     VDVSQDVIIQEEDCGTLRGLVAVELKNNEEVVASLYERILGRVSVHDIQHPLTGKHIV
                     SAGEEITENTAKIIQDSPIERVEIRSVLTCESKKGVCAKCYGRNLSTNRMVQKGEAVG
                     VIAAQSIGEPGTQLTLRTFHVGGIASNVATESKIISRYNGILEIEELRTVSVTEEGKT
                     HQIVVSRLAELKIIEPTTKVVLTTQNIPYGAKLYFQSGVTVEKGAVICEWDPFNAVVI
                     SEVAGKMTFESMVENITFKTESDEQTGLKERIIIESKDKSKVPAAHILDEKGEILRTY
                     ALPVGAHIAITEGSKIKIGQILLKMPRAVGKSGDITGGLPRVTELFEARNPSNPAIVS
                     EIDGEITFGKIKRGQREIVVTSKSGEVKKYLVPLSKQILVQENDFVRAGNPLSDGAIT
                     PSDILAIKGPTAVQEYIVNEVQDVYRLQGVKINDKHFEVIVRQMMRKVEIEDPGDTRF
                     LERQIVDKQEFMEENDRIWGKKVVVNPGDSEVLMAGQIIAPRKLRDENSMLKRKDLRT
                     VEVRDAMSATSSAILQGITRASLQTTSFMSAASFQETTKVLNDAAINGKVDKLEGLKE
                     NVICGHLIPAGTGQPELEKIVVASRDEYEKIMQAKRSVTDVNLKEAEEVSF"
     gene            complement(7230..8612)
                     /locus_tag="PKN90_00015"
     CDS             complement(7230..8612)
                     /locus_tag="PKN90_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNZ84701.1"
                     /translation="MKKCIYLLLFIFLSIICVSCISGENQGNQENIPDLEGILQRGKI
                     RVITLYGPLSYFNYKDNVMGYEYEFAQQLSQELGVEMEMIVAKNIANMIELLENGEGD
                     LIAYRVPRTLEYQQRVSFTDREYVTSQVVIQLQSDSILTDVLQLAGKEVYVPESSIYH
                     DRIVDLNNEIGGGIHIVAVNDSVSVDALIVQTAQQLIPMTIADADIARLNKTYFSSID
                     YSLSISFPQRYAWAVRNDNPELLAYINKWNAKNAKNMKLSSIYNKYFQRTKYFESEGF
                     EQKKLHQLSAFDDYFKKYASIVSWDWRLIAAIAYKESKFDPTIVSWAGAAGIMQLMPS
                     TARTMGFSEHDLHSPEQSIKAGVKYLQHLQEIFQQIPVKEERIKFVLAAYNSGPGHLF
                     DARALALKYGKDPNIWHDNVEIYVRLKSNPEYYTDEVCKHGYLRGDETANYVEFVENK
                     FEEYKTRIGK"
     gene            complement(8634..9371)
                     /locus_tag="PKN90_00020"
     CDS             complement(8634..9371)
                     /locus_tag="PKN90_00020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ84702.1"
                     /translation="MQQTTLEKILFLDIETVPQEATIKKMPESLAHLWMDKLDTLKRR
                     MPDRYDDDSSIDDLYRKEAGIYAEFGKIVCISVGIIYLKDGEKHCRIKSFFGHDEKTL
                     LTEFSGMLLKFMQSAEYQLCGHNIKEFDVPYIARRMLINGLPLPDVLQVAGKKPWEVK
                     FLDTLELWRFGDYKNYTSLKLLTAIFNIPTPKDDIDGSEVATVYYEHGDINRIAIYCQ
                     KDVVATIQVYLKLIGESIIQDKHIQLV"
     gene            complement(9377..9985)
                     /gene="udk"
                     /locus_tag="PKN90_00025"
     CDS             complement(9377..9985)
                     /gene="udk"
                     /locus_tag="PKN90_00025"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNZ84703.1"
                     /translation="MIIIGIAGGTGSGKSTVAKEILSQLPDGDIAFLPQDSYYKDSSH
                     LPMDKRIEINFDHPDSIEFDLLISHIKELKKGNSVQQPIYSYITCSRAEETVLIKPAK
                     IIVVEGILIFTDKELRKLMDLKVFVDADADDRLIRVINRDIVERGRSVNSVIERYQKV
                     LKPMHLQFIEPTKRYADLIIPEGGHNKVAISILQHYIDNKLR"
     gene            10130..>10210
                     /locus_tag="PKN90_00030"
     CDS             10130..>10210
                     /locus_tag="PKN90_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ84704.1"
                     /translation="MSVNKVILVGNVGKDPEVRYVDNNVAV"
BASE COUNT         3524 a         1669 c         1901 g         3116 t
ORIGIN      
        1 aattaacaag aaattagatt tagatatctc tcctgaaatt aaagtattaa caaaagaaga
       61 tattattgcc attatccgtt atctcattga attaataaat tctaaagctg acgtagatga
      121 cattgaccat ttaagtaatc gtcgtgtacg tacggttggt gaacaattat gcaatcaatt
      181 cagtgtagga cttgcacgta tggcacgtac tattcgtgag cggatgaatg tacgcgacaa
      241 tgaagtattt acgccaattg atttaatcaa tgcgaaagca atttcgtctg taatcaattc
      301 attttttggc accaatgcat tgtctcaatt tatggatcaa acgaatccgc ttgcagaaat
      361 gacacataaa cgtcgtatgt ctgcacttgg accaggagga ctttcacgag aaagagctgg
      421 ttttgaggtg cgtgacgttc actatacgca ttatggacgt ttgtgtccaa tcgaaacgcc
      481 agaaggtcca aatattgggt tgatctcttc tctttgtgta tatgctaaaa tcaatgattt
      541 aggttttata gaaacgccat accgtaaagt tgaggacaaa aaagtaaatt taaacaaaga
      601 ggatattatt tatctttctg cggaagaaga agaaaatatt attatcgcac agggtaatgc
      661 tccactagat gataatggaa actttatacg tacacgtgtt aaagctcgtc aggatagtga
      721 tttcccaatt gttgctccag aagaagttca attaatggat gtatctccta ctcaaatagc
      781 atcaattgca gcttcactta ttccattttt ggaacatgat gatgcaaacc gtgcgttaat
      841 ggggtcaaac atgatgcgtc aagcagttcc attgctacgt tgtgaagctc ctattgtagg
      901 aacaggattg gaaggtcaaa ttataaaaga ttcaagaact caaattacgg cagaaagaga
      961 tggtgtaatt gagtatgtag atgctacaac tataaaagta cgttacaatt actcagaaga
     1021 agaattattc gttcaatttg aaagttctgt aaaagaatac acattaccaa aatttcaaaa
     1081 gacaaaccaa agtactacaa ttgatcttag acccattgtt cataaaggag aaaaagtagt
     1141 tgctggacag atattaacag aaggatattc aactcaacaa ggtgaattag caatagggaa
     1201 aaacttaaaa gtggcattta tgccttggaa aggatataac tttgaagatg caattgttat
     1261 taacgaacgt cttgtacgcg aagatgtatt tacttctatt cacgtggatg agtactcttt
     1321 agaagtaaga gaaacaaaac gaggattaga agagctcact gcagatattc ccaacgtaag
     1381 cgaagacgct acaaaaaatt tagatgaaaa tggtattgtt cgtattggag ctttaattga
     1441 acctggagat attttaattg gtaaaattac tcctaaagga gaatcggacc catccccgga
     1501 agaaaaatta cttcgtgcta tttttggaga caaagctgga gatgttaaag atgcttcttt
     1561 aaaggctcat ccatcattaa ggggtgttgt tattgagaaa agattatttt caaaagcaca
     1621 aaaagcaaaa aaatccaaat cagcagataa agccattatg gacaaaattg aagctgaatt
     1681 tgaagaaaaa gcacatgcat tgaaattaaa attagttgaa aaacttttag tattaacaaa
     1741 tgggaaaacc tctcaggggg taaaagactt tttgggagct gaagtaattt ctaaaggtag
     1801 taaatttact caaaaacaat tgttagatgt agactactct actgtacaaa taagcaaatg
     1861 gacaagtgat agtgcaaaaa atgaacaaat caaaacactt atcatgaact atttgaaaaa
     1921 atacaaagag tatgatgccc aattgaagag agaaaaatat aatattacaa ttggggatga
     1981 actacctgcg ggtattattc aattagccaa agtatatata gcgaaaaaac gtaaagtaca
     2041 ggttggtgat aaaatggcag gaagacatgg taataaaggt atcgtgtctc gtattgtacg
     2101 tcaagaagat atgccatttc ttgaagatgg aagccctgtg gatatagtac tgaacccttt
     2161 gggtgtgcct tcgcgtatga acttaggtca aatttttgaa accgtattgg gatgggctgg
     2221 aaaagaatta aatgtaaaat tttctacgcc aatttttgat ggtgctacat tggaagattt
     2281 agacgaatgg actacaaaag caaacattcc tcgttatggt aagacatatt tatacgatgg
     2341 tggaacaggt gaaaaatttg accaaccagc gactgtaggt gttatctaca tgcttaaatt
     2401 aggacatatg gtcgaagata aaatgcatgc tcgttcaatt ggaccatact cacttattac
     2461 acaacaacca cttggaggaa aagctcaatt tggaggtcaa cgttttggtg aaatggaggt
     2521 ttgggctctc gaggcttttg gtgctgctca tattttgcaa gaaatattga ctgtaaaatc
     2581 agatgatgta atgggacgtg caaaaaccta cgaagctatt gtaaaaggag atcctatgcc
     2641 cgcacctggc ataccagaat ctctcaacgt attactacac gaacttcgtg gtttgggact
     2701 tagcattaat ttagaataaa ataagtggtt tcaagatatg ggaacattcc catatcttga
     2761 catctttttt aaataaaaaa tatatggcat ttagaaaaga aaataaagca aaaagcagtt
     2821 ttagcaaaat atcaataggg ttagcgtctc ccgaagaaat tttagaacac tctagtggag
     2881 aggttctaaa gcctgaaact attaactatc gtacttataa accggaacgt gatggattgt
     2941 tctgtgaacg catttttggt cctgtaaaag attatgaatg tcattgtgga aaatacaaac
     3001 gtattcgtta caaagggatt gtatgtgata gatgtggagt ggaagttact gataaaaaag
     3061 tacgtagaga acgtatgggt catattcaat tagtagtacc cgtagcacat atttggtatt
     3121 tccgttcctt acccaataag ataggttact tacttggtat atctactaaa aaattagatt
     3181 caattattta ttatgaaaga tatgttgtta tccaagcagg aacacaatct gatttggaag
     3241 taggagattt acttactgaa gatgaatatt taaatatcat ggatgcctta ccaagagaaa
     3301 attatctttt ggaagataca gaccctaata agtttatagc aaaaatggga gctgaagcta
     3361 tttatgatat gttatctcgt ttggatttag ataaaatttc ttacgagtta cgcgacaaag
     3421 ctagcaacga cacttctcaa caacgcaaaa cagaagcatt gaaacgctta caagtagtag
     3481 aagcttttcg tgcgtcaaag caacgcaaca aaccagaatg gatggtcgta aaagtagtgc
     3541 cagttattcc accagaattg cgtccgcttg tgcctcttga tggaggacgt tttgcaactt
     3601 cggacttaaa tgatttgtat cgtcgtgtta ttattcgtaa caatcgtttg aaaaagttaa
     3661 ttgaaatcaa agctccagat gtaattctac gtaatgaaaa acgtatgttg caagaggctg
     3721 ttgactcatt atttgacaat accagaaaat ctactgctgt aaaagcagat gcaaacagac
     3781 cattaaaatc tttgtcagat agcttaaagg gaaaacaagg acgtttccgt caaaacttat
     3841 taggtaagcg tgttgactat tctgcacgtt cggtaattgt tgttggtcct gaattaaaaa
     3901 tgcacgaatg tggtttgccc aaagatatgg cagcagaact ttacaaacca tttgtaatta
     3961 gaaaactaat tgaacgtggc attgttaaga cggtaaaatc tgcaaaaaag attgtggata
     4021 gaaaagaccc tgtagtgtgg gatatattag agcacgtgat gaaaggacat cctgtgctac
     4081 taaaccgtgc tccaactctt caccgacttg gtattcaagc ttttcagcct aaaatgattg
     4141 aaggaaaagc tattcaactt caccctcttt catgtacagc atttaatgct gactttgacg
     4201 gagaccaaat ggccgtacat ttaccattag ggaatgaagc tgtgctagag gctcaaattt
     4261 taatgcttgc ttctcataat attttgaacc ctgcaaatgg agctcccatt acagtacctt
     4321 ctcaagacat ggtacttgga ctgtattaca tgacaaaagg cagaagtggt gcattaggtg
     4381 aagggcttaa gttttattct cctaaagaag ttattattgc ctacaacgaa cgtaaagtat
     4441 cgttacatgc aaacatatct gtaaaaacac aggatgtgga aaatggagta atcgttacca
     4501 aaatgataga aactactgta ggacgtgtga tattaaatga atatgttcct aaaaaagtgg
     4561 gttatatcaa cgaaatttta tcaaaaaaat cacttcgtga tattattggc aaaattatca
     4621 atgtgtgtgg tattgcagat acagcgaact ttttggatga tattaaaagc ctaggttacc
     4681 aaatggcatt caaaggctgt ctgtcattta acttagataa catcattatt ccaaaagaaa
     4741 aagaagaatt agttcaacaa ggatatgctg aagtagaaga aattttggct aactataata
     4801 tgggtttcat tacttacaat gaacgctata accaaatcat tgatacgtgg acacatatca
     4861 actctaagtt gtcaaatatt ttaatgaaac agctttcgac agacgatcaa gggtttaact
     4921 cagtatacat gatgttagat tctggtgctc gtggatcgaa agaacagatt aggcagttat
     4981 ctggtatgcg tggtctgatg gcaaaaccac aaaaatcagg agctgaaggg ggtcaaatta
     5041 ttgaaaaccc aattttatca aactttaaag aaggcttatc agtactggaa tactttatct
     5101 ctacgcacgg tgcacgtaag ggtcttgcag atacagcgtt aaaaacagca gatgctggat
     5161 atttaactag acgtttggtt gacgtatcgc aagatgtaat tattcaagaa gaagactgtg
     5221 gtactttgcg tgggttagtt gctgtggagt taaaaaacaa tgaagaagtc gttgcatctt
     5281 tatacgaaag aatattaggt cgcgtttctg ttcatgacat acaacatcca cttacaggca
     5341 aacacattgt atcagcagga gaagaaatta ctgaaaatac agctaagata attcaagatt
     5401 cgcctattga acgagtagag attcgttcgg tattaacctg tgagtcgaaa aaaggagttt
     5461 gtgctaagtg ttatggtcgc aatttatcga caaacagaat ggttcaaaaa ggagaagcag
     5521 taggtgtaat tgcagctcaa tcaattggtg aaccaggaac acagcttaca cttcgaactt
     5581 tccatgttgg gggtattgcc tcaaacgtag ctactgaatc taaaataatt tcaagataca
     5641 atggtatttt ggaaattgaa gagttacgta cagtgagtgt tactgaagaa ggtaagaccc
     5701 accaaattgt agtaagtcgt ttggcagaac tcaaaatcat tgagcctact acaaaggttg
     5761 ttcttacaac acaaaatatc ccatatggag ccaaattgta tttccaatca ggagtaaccg
     5821 ttgaaaaagg ggctgtaatt tgcgaatggg atccttttaa tgctgttgtt atatctgagg
     5881 ttgctggtaa aatgacgttc gaaagtatgg ttgaaaatat tacattcaaa accgaatctg
     5941 atgagcaaac tggcttgaaa gaacgcatca taattgaatc gaaagataaa agcaaggttc
     6001 ctgcggcaca tatacttgat gaaaaagggg aaattttacg aacatatgca cttcctgttg
     6061 gtgcacacat tgcgattaca gaaggttcta aaataaaaat tggacagatt ttactaaaga
     6121 tgccacgggc agtaggcaaa tctggagata tcacaggtgg tttgccacgt gtaacagaat
     6181 tgtttgaagc acgtaatcca tctaatccag ctattgtctc ggaaattgat ggtgaaatta
     6241 catttgggaa aattaagaga ggtcagcgcg aaattgtagt tacatcaaaa tctggagaag
     6301 taaaaaaata cttagttcct ctgtcaaaac aaattttagt tcaagaaaat gattttgtaa
     6361 gagctggtaa tccattatca gatggagcta ttactccttc tgacatatta gcaattaaag
     6421 gacctacggc tgtacaagag tatattgtaa acgaagttca ggatgtatat cgcctacaag
     6481 gagttaaaat taacgataaa cactttgaag taatcgtgcg tcaaatgatg cgtaaagtgg
     6541 aaatagaaga tccaggtgat acacgtttct tagaaagaca aattgttgac aaacaagagt
     6601 ttatggaaga aaacgaccgt atatggggta aaaaagtagt agtaaaccca ggtgattctg
     6661 aggtattaat ggcagggcaa attattgcac cacgtaaatt acgcgatgaa aacagtatgt
     6721 tgaaacgtaa agatttaaga acagttgagg tgcgtgatgc tatgagtgca acgtcaagtg
     6781 ctatattaca aggtattact agggcttcgt tacaaactac aagctttatg tcagcagcct
     6841 cgttccaaga aactactaaa gtattaaatg atgctgctat aaacggcaaa gtggacaagc
     6901 tagaaggatt aaaagaaaac gtgatttgtg gtcatttaat ccctgctgga acaggacaac
     6961 cagaactcga aaaaattgta gtagcttcgc gtgatgaata tgaaaaaatt atgcaagcta
     7021 aacgcagtgt aacagatgtg aatctaaaag aagcagaaga agtttctttc taacaatcat
     7081 atctccttca tacaaaaaag catcctattt tgttatagga tgctttttta ttgatattct
     7141 tttatgtgtt atagaatcta tgggaataat caaaaataac aagaccttat gaatcaaaaa
     7201 caataccaag attaaacttg atatatcaat catttaccta tacgtgtttt atattcttca
     7261 aatttatttt caacaaattc tacatagtta gctgtttcgt ctcctctgag atagccatgt
     7321 ttacatactt catcggtata gtattctggg ttcgatttta accttacata aatctctaca
     7381 ttatcgtgcc agatattggg atctttgcca tatttaagag ctaatgcccg agcatcaaat
     7441 aaatgaccag gtccagaatt atatgcagct aatacaaact ttattctttc ttctttaaca
     7501 ggaatttgtt ggaagatttc ctgtaaatgt tgtaaatact taactcccgc ttttatactt
     7561 tgctctggac tatgtaaatc atgttcacta aaacccattg tacgtgcagt tgatggcatc
     7621 agttgcataa taccagcagc acccgcccaa gatacaatgg taggatcgaa ttttgactcc
     7681 ttgtatgcaa tagcagcaat taaacgccag tcccaagaaa caatggatgc atatttctta
     7741 aaataatcat caaaggcaga aagttgatgt agtttttttt gttcaaatcc ttcagactca
     7801 aaatatttgg tacgttgaaa atacttatta taaatactcg acaacttcat gtttttagca
     7861 tttttagcat tccacttgtt gatatatgct aacaattcag gattatcatt tctcacagcc
     7921 caagcataac gttgtggaaa actgatagat aaggaataat caattgaaga aaaatatgtt
     7981 ttattcaatc tagcaatatc agcatcggca attgtcattg gaataagctg ttgtgctgtt
     8041 tgtacgataa gggcatctac agatacagaa tcattaactg ctacaatgtg tatacctccc
     8101 cctatttcat tattcaaatc gacaattcta tcatgataga ttgatgattc aggcacatat
     8161 acttcttttc cagctaattg tagtacatca gtaagtatgc tatctgattg taattgtata
     8221 acaacctgag atgttacata ttctctatcc gtaaagctta cacgttgttg atactccaat
     8281 gttcgtggaa cacgataagc aattaaatcg ccctctccat tttccaagag ttctatcatg
     8341 ttggcaatat ttttagcaac aatcatctcc atttctacac ccaattcttg acttaattgt
     8401 tgtgcaaact catattcgta ccccattaca ttatccttat agttaaaata cgatagagga
     8461 ccatataacg taataacacg aatttttcca cgttgcaaaa taccctctaa atctggaata
     8521 ttttcttgat ttccctggtt ttctccacta atacaggata cgcaaattat ggaaagaaaa
     8581 ataaatagca ataaatagat acattttttc attcatattt attttataaa catttaaacc
     8641 aattgtatgt gtttatcttg gatgatagat tctccaatga gttttagata tacttgaatt
     8701 gttgccacga catctttctg acaatagatt gctattctat ttatatctcc atgttcgtaa
     8761 tacacagttg ctacctcgct tccatcaata tcgtctttgg gagtgggaat attaaaaatt
     8821 gctgttaaaa gttttaatga ggtataattc ttataatctc caaaacgcca tagttctaag
     8881 gtatcaagaa acttaacctc ccacggtttt tttccagcca cttgcaatac atcaggcaat
     8941 ggtaagccat taattaacat tcgtcgagca atatatggca catcaaactc tttgatatta
     9001 tgaccacata attgatattc agccgattgc ataaatttta ataacattcc tgagaattcg
     9061 gttaagagcg ttttctcatc atgcccaaaa aatgacttaa tacgacaatg tttttcgcca
     9121 tcttttaggt aaatgattcc gacagaaatg caaacaattt ttccaaattc agcatatata
     9181 ccagcttctt ttctatacaa atcatcaata gagctatcat catcatacct atcaggcatt
     9241 ctacgtttta gggtatcaag tttatccatc cacaaatgag ctaaggactc aggcatcttt
     9301 tttatagttg cctcttgggg aacagtttct atatccaaaa ataatatctt ttcaagtgtt
     9361 gtttgttgca tatcatttat cttaatttgt tgtctatata atgttgtaaa atactgatag
     9421 ctactttatt gtgtcctccc tctggaataa ttaaatctgc atatcgttta gttggttcaa
     9481 taaattgtaa atgcattggt tttaatactt tttgataacg ttctattaca gaattaaccg
     9541 aacgtcctct ttctacaata tctctattaa taaccctaat taacctatcg tcggcatctg
     9601 catcgacaaa tacttttaaa tccattagtt ttcgtaattc tttatcggta aaaatcagaa
     9661 ttccttccac aacaataatc tttgcgggtt ttataagcac tgtttcttca gcacgagagc
     9721 aagtaatata tgaatagata ggttgctgaa cagaatttcc ttttttcaat tccttgatat
     9781 gggaaattaa caaatcaaat tcgatagaat ctggatgatc aaaatttatt tcgatgcgtt
     9841 tatccattgg caaatgacta ctgtccttat aataagaatc ttgtggtaaa aaagcaatgt
     9901 cgccatcagg caattgactc aatatttcct ttgctacagt actttttcca gaaccagttc
     9961 cgccagcaat tccaataatt atcatatttt cagtatatta aattaagaat gcactaaggt
    10021 agaaaaatat ttttaagaaa gataaaaaaa gatgcaaaaa atactatttt ttatttatct
    10081 ttgcaccgat ttaattaaca ataataaatt tacttaatct agaaaaaaga tgtcagttaa
    10141 caaagtaatt ttggtcggaa atgtgggcaa agaccctgaa gttcgttacg ttgataacaa
    10201 tgtagcagtt
//
LOCUS       DAOPUR010000002         9298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_7459_length_9298_cov_3.356702, whole genome shotgun sequence.
ACCESSION   DAOPUR010000002 DAOPUR010000000
VERSION     DAOPUR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7459_length_9298_cov_3.356702"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKN90_00035"
     CDS             complement(<1..923)
                     /locus_tag="PKN90_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNZ84705.1"
                     /translation="MTNIHILWVDDEIDLLRSHILFLESKGYTVTTANSGHDALALCK
                     KETYDIAFLDENMPGLSGLETLSEIKKMQPALPIIMITKNEEEDIMEQAIGKKIADYL
                     IKPVNPNQIVMAIKKCLHSKQLISDKVTSSYQSAFTQIGMEINDTYTFEDWKKVYKHL
                     VYWELELEHADNQMDSLLMMQKNEANANFTKYIRKNYTSWFEPNDDKPLMSPDIFKNK
                     IFPLLDKGEKVCFVVIDNLRFDQWKVLQKELSQWFYTADDDLYCGILPTATQYARNAI
                     FAGLMPLQIQKMFPHYWIEEEDEEGKNMKEE"
     gene            complement(1010..1960)
                     /locus_tag="PKN90_00040"
     CDS             complement(1010..1960)
                     /locus_tag="PKN90_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ84706.1"
                     /translation="MQFLDKYYKWIKIVIMVCAYGYLAYILLHFDNYEDFTSNFTNLN
                     AQKFIFLLVGFCLLPINLFVESLKWKTLVSVMQRIPIRVAYKAVVAGITTGFFTPNRI
                     GEPLGRIMFLDSQNRSQAIALSYVGAFGQSFATFLCGVIAGFLLIFSPAFSAYQNSTG
                     MQAGAVLFLVMFISLYFSLPYLCRKITKISKFDKVNYLLEAISYISLKRLFVVSALSL
                     LRYTIYVSQYYFLLLFFGVDISMYEAFVAIPINYLLVSITPSIAFAEMGIRGSYAVII
                     IGAFSHNPPAVALAGVTVWLINYVIPMITGSLLLTAKKKL"
     gene            complement(1966..2457)
                     /locus_tag="PKN90_00045"
     CDS             complement(1966..2457)
                     /locus_tag="PKN90_00045"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNZ84707.1"
                     /translation="MLVRFGKIIRNYYIQFLDFLYPLFKQWMHKGLFYYLASGGINVG
                     TNWIMYFVVHNFVVQKQIVDLGFIAFSPHIASLIITFPFTFLLGFGLSKYVSFAESTV
                     RTKTQFFRYAGIIAFNFLVSYLSMKLLVEWFGIYPTPSNMITTIITSVVSYFSQKHYS
                     FKK"
     gene            2555..3349
                     /gene="rsmA"
                     /locus_tag="PKN90_00050"
     CDS             2555..3349
                     /gene="rsmA"
                     /locus_tag="PKN90_00050"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNZ84708.1"
                     /translation="MSYVKPKKHLGQHFLTDQHVARSIAETLRISEPTKVLEIGPGTG
                     VLTQYLLENELIDLKVIEIDGESVEYLQKEFSALHNRIFFQDFLTTDIPQLYGSETFS
                     VIGNFPYNISSQIFFKILENKHQIPCVCGMLQKEVAERLASKPGKKTYGILSVLLQAY
                     YDVEYLFTVPPTVFNPPPKVNSAVIRVTRNATQQLECNETLFKTVIKTAFNQRRKTMR
                     NSLKGIITVSSPIVTESIFDKRPEQLSVNEFVTLTNLIEPYIQSQH"
     gene            3451..4725
                     /gene="purD"
                     /locus_tag="PKN90_00055"
     CDS             3451..4725
                     /gene="purD"
                     /locus_tag="PKN90_00055"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNZ84709.1"
                     /translation="MKHTILLLGSGGREHAIAWKLTQSTKLNRLFIAPGNAGTAQLGE
                     NISINPEDFVAVQTFVEKNDVTMVIVGPEAPLVAGIVDFFKATPTIAHIPVLGPSKYA
                     AQMEGSKEFAKEFMKRHAIPTAGYLSVTKDNLAQGFAFLETLKAPYVLKADGLAAGKG
                     VLIISDLNEAKTELAAMLDGKFGKASSTVVIEEFLDGIECSVFVLTDGDKYVILPEAK
                     DYKRIGEGDTGPNTGGMGAISPVSFANATFMQQVEERIIRPTINGFRTENIDYKGFVF
                     FGLIKVNNEPYVIEYNARMGDPETEAVMLRLQNDLLTLVEATFDGTLDKQVMKFDTRF
                     AVTVMLVAGGYPGDYQKGDVITGCELVEDSIAFHAGTTLQNNDIVTNGGRVIAISSYG
                     NTKEEALACSFKNAHKINYKGKYFRSDIGFDL"
     gene            4722..5705
                     /locus_tag="PKN90_00060"
     CDS             4722..5705
                     /locus_tag="PKN90_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84710.1"
                     /translation="MNWREERHQILAPWSIFLITGVFAIAFWIKSFLSYDMVNFPTEK
                     DILYYYLTQIIPFDSFLAKIIAFLLVVFIAVITFFANNKYFFIKNKSALIIMFMVLLM
                     GLNDPSHNLNPGIIGLTFLLFAFIRFLGMHGKLHTGARSFDIGLYISIGSLFYDRMLF
                     FIPLFYIGFYIYQQLHIKNILATFFGITFPYLLTGGMCFLFDDFDNFVQRIPFSISHT
                     TDTNIGHIIYYGLLGLVTLLAFFNFILKYSNEKIKTRNTIFFIYLLLLGTIVSIFFSN
                     HLFLFELPFISFFLSIVFAHFFANNTSRFSRFIFYAFWTIGIIWFLVSFIL"
     gene            5707..6165
                     /locus_tag="PKN90_00065"
     CDS             5707..6165
                     /locus_tag="PKN90_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNZ84711.1"
                     /translation="MSEILYFLTEYGYIGLFFAAFLAATILPFSSEVVFTGLLYSTQS
                     DVWLCILAATLGNWLGGITCYWLGRLGKTEWISRYLRVPPDKVTHWQNKIQGKAALAA
                     FFSFLPGIGDLIAIAAGYLRANFWLTSTSILIGKFLRFVVWVYITYWYWA"
     gene            6388..6843
                     /gene="rplM"
                     /locus_tag="PKN90_00070"
     CDS             6388..6843
                     /gene="rplM"
                     /locus_tag="PKN90_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNZ84712.1"
                     /translation="MNTLSYKTISANKATVQKEWVVVDATDQVLGRMCSKVAKLLRGK
                     YKPSFTPHVDCGDNVIIINADKVKMTGNKWTDKVYLRYTGYPGGQREMTPQRLQAKGS
                     DRLFNKVLKGMLPKNRLGDKLLGNVYIYDGAEHPHQAQTPKTIDLNVLK"
     gene            6849..7235
                     /gene="rpsI"
                     /locus_tag="PKN90_00075"
     CDS             6849..7235
                     /gene="rpsI"
                     /locus_tag="PKN90_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNZ84713.1"
                     /translation="MEITNAIGRRKAAVARVFVREGKGNIVINKRGIDSYFPSGILQY
                     IVKQPLAKLDVVEKYDIQVNLNGGGFKGQAEALRLAIARALVKINPEDKPALKSEGFM
                     TRDPREVERKKPGRPKARKRFQFSKR"
     gene            7393..8232
                     /gene="rpsB"
                     /locus_tag="PKN90_00080"
     CDS             7393..8232
                     /gene="rpsB"
                     /locus_tag="PKN90_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNZ84714.1"
                     /translation="MSRTNFQDLLDAGAHFGHLKRKWNPAMSPYIFMEHNGIHIIDLH
                     KTVVKIDEAAEVLFKIAKQGKKVLFVATKKQAKDIIADKASSVNMPYITERWAGGMLT
                     NFPTIRKAVKKMSVIDKMATDGTFENLSKREKLQISRQREKLEKNLGSIADLTRLPAA
                     LFVVDVMKEQIAVKEANRLGIPVFGIVDTNSNPNNIDYVIPANDDAAKSIEIILKAVV
                     EAIQEGLQERKNDKLELEASEVPVDKMKKSKAPSKVRTKRGIDESALNANVADKFLKG
                     EKE"
     gene            8343..9167
                     /gene="tsf"
                     /locus_tag="PKN90_00085"
     CDS             8343..9167
                     /gene="tsf"
                     /locus_tag="PKN90_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNZ84715.1"
                     /translation="MAVTMAEISKLRTMTGAGMMDCKNALTESNGDFDKAVEIIRKKG
                     QAVAAKRTDREASEGCVLAASKEAFAACVALKCETDFVAKNADFIALTQTILDIVMDK
                     QPTTLEEVLALPMGNGTIASSITDRSGITGEKMELGYYEWVKGASTVSYIHPGNKLAT
                     IAAFNLAGTDQTVSRDVAMQIAAMNPISLNRDSVPANVIATELEIGRDKARQEGKPEA
                     MLDKIAEGRLNKFYQESTLLEQAFIKDGKITVQNYLKQANKDLTVTDFKRVTLNQE"
BASE COUNT         3223 a         1620 c         1591 g         2864 t
ORIGIN      
        1 tcttcttcct tcatattttt cccctcctca tcctcctctt ctatccaata gtggggaaac
       61 attttttgaa tctgcaaagg cattaatccc gcaaaaattg catttcgtgc atattgagta
      121 gcagtaggta aaataccaca atacaaatca tcatctgcag tgtaaaacca ttgagacaat
      181 tctttttgta atactttcca ctgatcgaaa cgcaaattat caatgaccac aaagcacacc
      241 ttttctcctt tgtctaacaa tggaaatatt ttattcttaa atatatccgg actcatcaaa
      301 ggtttatcgt cattgggttc gaaccacgat gtgtaattct ttctaatata ctttgtaaaa
      361 tttgcattgg cttcattctt ttgcatcatc agcaacgaat ccatctgatt atctgcatgc
      421 tctagctcca actcccaata cactaaatgt ttgtaaacct ttttccaatc ttcgaatgta
      481 tacgtatcat ttatctccat accgatttgg gtaaaagcag actggtatga actcgttact
      541 ttatctgaaa ttaattgttt ggaatgcaaa cattttttaa ttgccatcac aatttggttg
      601 ggatttacag gctttattaa gtaatctgca atttttttcc caattgcctg ctccataata
      661 tcctcttcct catttttcgt tatcatgata ataggcaatg ctggttgcat tttttttatc
      721 tctgacaagg tttcaagacc gcttaatcct ggcatatttt cgtctaagaa agcaatatcg
      781 tacgtttctt ttttgcacaa tgccaatgca tcatgtccac tattagccgt agttaccgta
      841 tatcctttag actctaaaaa tagaatatga gaccgcaata agtcaatttc atcatctacc
      901 cataaaatat gtatatttgt cataaatggt tacgtttggt gatttgttct tttttacaaa
      961 gatatgacat ttacaggtat tcttcaatta tttaagcagt attatctggt taaagtttct
     1021 tttttgcggt taaaagaagt gaaccagtta tcatgggaat aacataattt attagccaaa
     1081 ccgtaactcc agccaaagct actgcaggag gattgtgtga aaaagctcct atgataataa
     1141 cagcatacga tcctctaatg cccatttcgg caaatgcgat tgaaggtgtt atagatacaa
     1201 gcaaataatt aattggtata gcgacaaaag cttcgtacat tgaaatatct accccgaaaa
     1261 acaataataa aaaataatac tgagaaacat atatcgtata cctaagtaaa gaaagtgctg
     1321 aaacaacaaa aagtctcttt agagaaatat aggatatagc ttcaagcaaa taattcactt
     1381 tatcaaattt actaatcttt gtaatttttc tacacaaata cggtagcgaa aaatacaaag
     1441 atataaacat cactaaaaat aatacagcac ccgcttgcat acccgtagaa ttttgatacg
     1501 cactaaatgc tggtgaaaaa atcaacaaaa aacctgcaat gactccacat aaaaacgtag
     1561 caaaactttg accaaaagca ccaacgtagg aaagtgctat tgcttgcgaa cgattttgcg
     1621 aatccaaaaa cataatccga ccaagaggtt ctcctattct atttggtgta aaaaaaccag
     1681 tggtgatacc agctacaaca gccttgtagg ctacccgaat aggaattctt tgcataacag
     1741 aaaccaatgt tttccatttc agagattcta caaatagatt aattggcaat aggcagaaac
     1801 caacaagcaa gaatataaac ttttgagcgt ttaagtttgt aaaatttgaa gtaaaatctt
     1861 cataattatc aaaatggagt aaaatatatg ccaaatatcc ataggcacac accataatca
     1921 ctattttaat ccacttgtaa tatttatcta aaaattgcat ctctcttact ttttaaacga
     1981 atagtgtttt tgagaaaaat aactaaccac actggttatg atagttgtaa tcatattaga
     2041 tggcgttgga tatataccaa accattctac taataatttc atagaaagat aacttaccaa
     2101 gaagttaaac gcaattatac cagcatatcg aaaaaattgc gtcttcgttc gcactgtcga
     2161 ttcggcaaaa cttacatatt tcgataatcc aaaacccagt aaaaaggtaa acggaaaagt
     2221 aataatcaaa ctcgcaatat gcggactaaa ggcgataaat cctaagtcaa ctatctgttt
     2281 ttgcaccaca aagttatgca ctacaaaata cataatccag ttagtgccca cattaatacc
     2341 acccgaagct aaataataaa acagcccttt gtgcatccac tgtttaaata acggatacaa
     2401 aaaatcgaga aattgaatgt aataattgcg tattattttg ccaaaacgaa ctaacatact
     2461 tttaggtact cgctttttga ttatctttgc aaagataaac tattttaaaa cgaaagcata
     2521 tttttgcttt tattatttgc taaagatata cactatgtca tacgtaaaac caaaaaaaca
     2581 ccttggacaa catttcctta ccgaccaaca cgtagctcgt agcattgccg aaacattgcg
     2641 aattagtgag cccacaaaag tactcgaaat aggtcctggc acaggcgtat tgacacagta
     2701 tttattggaa aacgaactca ttgatttgaa agtaattgag atagatggag agtctgttga
     2761 atacttacaa aaagaatttt ctgccttgca taaccgcata ttttttcaag acttcttgac
     2821 aacagatata cctcagcttt acggatcaga aactttttct gttataggca atttcccgta
     2881 taacatatct agtcagatat tttttaaaat acttgaaaac aaacaccaaa tcccttgtgt
     2941 gtgtggtatg ttgcaaaagg aagtggcgga acgattggcg tcgaagccag ggaaaaagac
     3001 atatggaatt ttaagtgttt tattacaagc atattacgat gtggagtatt tatttacggt
     3061 acctcctaca gtgtttaacc caccgccaaa agtcaactcg gcagttatac gtgttacacg
     3121 caacgctact caacaactag aatgcaacga gaccttattt aaaactgtta tcaaaacagc
     3181 tttcaatcaa cgaagaaaaa caatgcgaaa ttcgctaaaa ggcatcataa cagtatcttc
     3241 tcctattgta acagaaagca tattcgacaa acgtccagag caactttctg taaacgaatt
     3301 tgttacactt actaatttaa ttgagccata tattcaaagt caacactaac acatatgtat
     3361 acatactaat tacaagaaaa caattagtat ctaaaaaaga aaaaaattgt acctttgtaa
     3421 cgtcaacaca taaagcaaat ttttacatat atgaaacaca caatactact tttggggtca
     3481 ggaggcagag aacacgccat cgcttggaaa ttaactcaaa gtacaaaact taatcggtta
     3541 tttatcgccc ctggcaatgc aggtaccgct caacttggag aaaacatctc cataaacccc
     3601 gaagactttg tggcagtaca aacatttgta gaaaaaaatg atgttaccat ggtaatcgtt
     3661 ggtccagaag ctcctttggt tgcaggtatt gtagattttt ttaaagcaac tcctacgata
     3721 gcacatatac ccgttcttgg tccttcaaaa tacgctgcac aaatggaagg cagtaaagaa
     3781 tttgccaaag aatttatgaa aagacatgct ataccaacag ctggatatct ttcggttaca
     3841 aaagacaatt tggctcaagg gtttgcattt ttggaaacat taaaagcccc ctatgtgtta
     3901 aaagcagatg gattagcagc tgggaaaggg gttttgatta tcagcgactt aaatgaagca
     3961 aaaacagaat tagcagctat gcttgatggc aaatttggca aagcaagctc aacagtagtg
     4021 atagaagaat ttttagatgg aatcgaatgt tccgtgtttg tactaactga cggagataaa
     4081 tatgttattc ttcccgaagc caaagactac aaacgaatag gcgaaggcga tacaggtcca
     4141 aatacaggag gtatgggggc aatttctccg gtaagctttg ccaacgctac ctttatgcaa
     4201 caagtagaag aacgaattat tcgtccgact ataaacggat ttcgcacaga aaacattgac
     4261 tacaaaggat ttgtgttttt tggtttgata aaagtaaaca atgaacctta cgtaatagaa
     4321 tacaatgcac gtatgggtga tcccgagaca gaagcagtga tgctacgctt acaaaatgat
     4381 ttacttacac tagttgaagc aacgtttgat ggcactttag ataaacaagt aatgaaattt
     4441 gatacccgat ttgctgttac tgtaatgtta gtagcaggag gatatcctgg cgattatcaa
     4501 aaaggcgatg tgattacagg ttgtgaactt gttgaagaca gtattgcatt tcatgcaggc
     4561 accacacttc aaaacaacga tattgtaacc aacggaggac gtgtaatagc aataagttca
     4621 tacggaaata cgaaagaaga agcattggct tgctctttca aaaatgcaca taaaatcaat
     4681 tacaaaggaa aatattttcg ttcagacata ggatttgatt tatgaattgg agagaagagc
     4741 gacatcaaat actagcaccg tggtccatat ttttgattac gggggttttt gctattgcat
     4801 tttggatcaa gtctttcctg tcatatgata tggtaaattt tcctactgaa aaagacatcc
     4861 tatattatta tcttactcaa atcattcctt ttgattcatt tttagccaaa attattgcgt
     4921 ttttgctagt tgttttcatt gcagtaatta cgttttttgc caacaataaa tatttcttta
     4981 taaaaaataa atcagcctta atcattatgt ttatggtttt gttgatggga ctaaacgacc
     5041 cttcgcacaa ccttaaccca ggtattattg gcttaacctt tcttctgttt gcctttatac
     5101 ggtttttggg catgcatggc aaattacata caggagcacg ttcttttgat attggattat
     5161 atatttcaat cggaagttta ttttatgata gaatgctctt ttttattccg ttgttttaca
     5221 tcggttttta tatttaccaa caattacaca tcaaaaatat actagctacc ttttttggaa
     5281 ttacttttcc ttatttactc acaggcggta tgtgtttttt atttgatgat tttgataatt
     5341 ttgttcaacg tattcccttt agcatatcac ataccacaga cacaaacatt ggacacatta
     5401 tttactatgg attattgggg ttagtaacgt tgctcgcttt ctttaatttt atactaaaat
     5461 acagtaacga aaaaatcaaa acacgtaata ccatcttttt tatttacctt cttctattag
     5521 gtactattgt aagtatattt ttttctaacc atcttttttt gtttgagctt cctttcattt
     5581 cgttcttctt gagtattgtt tttgcacatt tctttgcaaa taatacttct cgtttttctc
     5641 gatttatttt ttatgcattt tggactattg gtattatttg gtttttagtc tcatttatat
     5701 tataaaatgt cagaaatact ttatttttta actgaatatg gttatatcgg actttttttt
     5761 gccgcttttt tagctgcaac catcttgcca tttagttctg aggtagtatt tacgggatta
     5821 ctatatagta cccaatcgga tgtttggcta tgtattttag ctgctacctt aggaaattgg
     5881 ttaggaggga ttacctgtta ttggctaggg agattaggaa aaaccgaatg gattagtcgc
     5941 tatttacgag ttccaccaga caaagtaaca cattggcaaa ataaaataca aggcaaagcc
     6001 gctttagctg ccttcttctc ttttttacca ggtattggcg atttaatagc catagcagca
     6061 ggttatttac gtgctaattt ttggcttacc agcacatcta tattaatagg aaaatttttg
     6121 cgttttgtgg tctgggtgta tattacttat tggtattggg catagtaatg caaaaaaaca
     6181 ccttccccta aaacaaacaa aaacacacaa atgtaaataa aattgttgaa aagtttttct
     6241 gtttcagaaa aatacagtat ttttgcagtc cgattattcc tccacgcacg taagtgttta
     6301 gtttttagcg agtaagaatc ttttttagcc tgaagacctt agtttgcgag aaacgaaaga
     6361 gtgtaaaatt tttaataaat tattattgtg aataccttaa gttacaaaac tatttctgct
     6421 aataaagcca ctgtacaaaa agagtgggtt gtagtagatg ctaccgacca agtgttgggt
     6481 cgtatgtgtt ctaaagtagc aaaattgttg agaggaaagt ataaaccatc tttcactcct
     6541 cacgttgatt gcggtgataa tgtaatcatt atcaatgcag ataaagtaaa aatgactggc
     6601 aacaaatgga cagataaagt ctatcttcgc tacacaggat atccaggtgg tcaacgcgaa
     6661 atgactcctc aacgtttgca agcaaaaggc tctgacagac ttttcaataa agtactgaaa
     6721 ggtatgttgc ccaaaaatcg cttgggagat aaattattag gcaatgtgta tatttatgat
     6781 ggagcagagc atccacatca agcacaaact ccaaaaacaa ttgatttaaa cgttcttaaa
     6841 taggaaatat ggaaattaca aatgcaatag gaagaagaaa agccgctgta gctcgcgtat
     6901 ttgtgagaga aggtaaaggc aatattgtta tcaataaaag aggtattgat tcatattttc
     6961 catcaggaat tttacagtat atcgtaaaac aaccacttgc taaattagat gtggtagaaa
     7021 aatacgatat tcaggtaaac ttaaatggtg gtggttttaa aggacaagca gaagcattgc
     7081 gtttggcaat cgctcgtgca ttggttaaaa tcaacccaga agacaaacct gcattgaaat
     7141 cagaaggctt catgacccgt gatccacgcg aagtggaacg taaaaaacca ggtcgtccaa
     7201 aagctcgcaa aagattccaa ttcagcaaac gttaatattc gaatactcgt tattacctgg
     7261 tatttggcaa gggtttatat ccaccaacag ccagtaatac ggtaggttta gtatctaaat
     7321 tatagggact ttgttgcaca aactacccta taattgaata aaagcagaaa gtaaacaaca
     7381 aaaaacaaac acatgtcaag aacaaatttt caagatttat tagatgcagg tgcacatttt
     7441 ggtcacctaa aaagaaagtg gaatccagca atgtctccat acatttttat ggaacacaat
     7501 ggtattcaca tcattgattt acacaaaaca gttgtaaaaa tcgatgaagc agccgaagtt
     7561 ttattcaaaa ttgccaaaca aggtaaaaaa gtattgtttg tagcaacaaa aaaacaagca
     7621 aaagatatca ttgctgacaa agcaagttct gtaaatatgc cttatatcac agaacgttgg
     7681 gctggtggta tgcttaccaa cttccctact atccgtaaag ccgtgaaaaa aatgtctgtt
     7741 atagataaaa tggctactga tggtacattt gaaaatcttt ctaaaagaga aaaattacaa
     7801 atctctcgtc aaagagaaaa acttgaaaaa aacttaggta gtattgctga tttaacacgt
     7861 ttacctgcag cgctatttgt agttgacgta atgaaagagc aaattgcagt aaaagaagct
     7921 aatcgtttgg gtattcctgt ttttggaatt gtagatacaa actctaaccc caacaacatt
     7981 gactatgtta ttcctgcaaa tgatgatgca gctaaatcaa ttgagattat cttaaaagca
     8041 gtggttgaag caattcaaga aggcttacaa gaaagaaaaa atgataagct agaattggaa
     8101 gcatctgaag tgcctgttga taaaatgaaa aaatctaaag ctccttcaaa agtaagaacg
     8161 aagagaggta ttgatgagtc agcacttaat gcaaatgttg cagataaatt cttaaaagga
     8221 gaaaaagagt aatatttttt cttttatatt tatgaatagg ttacaactca aactctttct
     8281 ttaaagaagt ggtggcggtt gtaacttttt tttttgacac taaacaaata aaacatacta
     8341 ttatggcagt aacaatggca gaaatatcca aattacgcac aatgactggt gcgggaatga
     8401 tggattgcaa aaatgctctt acagagtcga acggtgattt cgacaaagca gtagaaataa
     8461 ttagaaaaaa aggacaagca gtagccgcaa aacgtaccga cagagaagct tctgaaggtt
     8521 gtgtacttgc agcatctaaa gaagcgtttg cagcatgcgt agctctaaaa tgcgaaacag
     8581 attttgtggc taaaaatgca gattttatag cattaacgca aacaattctt gatatcgtga
     8641 tggacaaaca accaacaacc cttgaagaag tgttggcttt accaatgggt aatggtacta
     8701 ttgcctcatc tattacagat agaagtggta ttacaggcga aaaaatggag cttggttatt
     8761 acgaatgggt aaaaggtgct agcactgtat cgtacataca cccaggaaat aaattagcta
     8821 ctattgcagc ttttaacctt gcaggaactg accaaacagt atctcgtgat gtggctatgc
     8881 aaattgcggc aatgaatcca atttctttaa atagagattc tgtacctgca aatgttattg
     8941 ctactgaatt agaaattggt cgtgataaag cacgccaaga aggtaaacca gaagctatgc
     9001 ttgataaaat tgcagaagga cgtttaaata aattctacca agaatctact ttattggaac
     9061 aagcttttat caaagatggt aaaataactg tacaaaacta tttaaaacaa gcaaacaaag
     9121 atttaactgt taccgatttc aaacgtgtaa cattgaatca agagtaagct cttgtgataa
     9181 taaacataaa aaatggagac tgaaaaatca gcctccattt ttttatagag ttgtactgta
     9241 ctatgaaatt tttacgccat ttttcactaa atcgctaggc gaaattacca ccaaacgt
//
LOCUS       DAOPUR010000003         9096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_7649_length_9096_cov_3.348413, whole genome shotgun sequence.
ACCESSION   DAOPUR010000003 DAOPUR010000000
VERSION     DAOPUR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7649_length_9096_cov_3.348413"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PKN90_00090"
     CDS             complement(<1..1178)
                     /locus_tag="PKN90_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ84716.1"
                     /translation="MRFFSLTLIFITLVVPVAIATNNQEKLSTQVVDRSAQQPDFDYY
                     FAEALRQKHLGNTAESIDLFLKSYDINPTSAIVSFELGNLFLQQKADDMGFGYIKNAA
                     ELAPQNYFYQEKLALLYRSNGDFSKAIEVLERIQRLFPDKDEVLYELSQLYTQNGQHK
                     KSIQKLDLLEKRIGLNKAITYDKAVQYVLLKNIKQAHKEIDAMLKKTPNDHALWILKG
                     DIEYERKKYGNAKKYFQKATDIQPESGYVYISWARYYEAIKDFSTSEEYLYKLFGAKD
                     ISFQDKMHYLEILTSYYSKRMDGIEKMHNLYTSLLQSHPMEYVAHTAYAEFLLQQKLV
                     PEAIEHFYTAALLHPSCLECWVYLLQYYTEKNENEKAYQIAKEAIEAVPNHPVLLFYS
                     G"
     gene            complement(1188..1622)
                     /gene="dut"
                     /locus_tag="PKN90_00095"
     CDS             complement(1188..1622)
                     /gene="dut"
                     /locus_tag="PKN90_00095"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNZ84717.1"
                     /translation="MQINIVNKSKHDLPEYATPLSAGMDIRANLSEPITLLPLQRKLI
                     PTGLYIELPKGYEAQIRPRSGLALKKGITVLNTPGTIDADYRGEIGVILVNISDEPFV
                     VNDGERICQMVVAEHATVRWQQVEQLDETQRGTGGFGHTGKK"
     gene            complement(1658..3763)
                     /gene="rnr"
                     /locus_tag="PKN90_00100"
                     /pseudo
     CDS             complement(1658..3763)
                     /gene="rnr"
                     /locus_tag="PKN90_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967281.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(3791..4540)
                     /locus_tag="PKN90_00105"
     CDS             complement(3791..4540)
                     /locus_tag="PKN90_00105"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNZ84718.1"
                     /translation="MGFLGSFLGGALGFTVGGPIGAIIGVFLGSAISSNYGEKKKKYT
                     SQNDFKLSLLILIATVLKADGKVTKSELQETKAFLRANFGEEEALEALQILKNILEQD
                     YDYKSICLQINQGLNYSSKLQLLQILFKISSADTIESSELRLLQTMATLMGISMGDFQ
                     SIQAIYSQHQHFHGQVKDTRKELDLAYQILEIDKNASDEEIKKAYRRMAMKYHPDKVS
                     TLGDEFKEIATEKFKAVKNAYELIKKEKGFA"
     gene            4768..6003
                     /locus_tag="PKN90_00110"
     CDS             4768..6003
                     /locus_tag="PKN90_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNZ84719.1"
                     /translation="MVLNYIWIGFFLIAFVVALFKLLVFGDMTIFPNIMNSTFEYAKM
                     GFELSLGLTGVMTLWLGVMKIGENGGAIKLLSKGISPLFNKLFPEIPKNHPVHGSMVM
                     NIAANMLGLDNAATPLGLKAMNELQELNKTKDTASNPMIMFLVLNTSGLTIIPISIMV
                     YRAQMGAADPSDIFIPILLSTFVSTLAAIIAVSIAQKINLFTKTILLYLGSITLLLTG
                     VIWGFTVLSSEKLNMVSSVAANIILFSIIIGFILLAIRKKINVYDSFIEGAKDGFSIA
                     VKIIPFLVAILVGIGVFRASGAMDSLMSGIAWLFTSIGLNANFVEALPVAFMKPLSGA
                     GARGLMIDAMNQHGVDSFVGRLACTFQGATDTTFYIIAVYFGSVGIRKVRHAVTCGLI
                     ADLAGIIAAIFIAYLFFHT"
     gene            6012..6443
                     /gene="ybeY"
                     /locus_tag="PKN90_00115"
     CDS             6012..6443
                     /gene="ybeY"
                     /locus_tag="PKN90_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNZ84720.1"
                     /translation="MAVHFIFDDTKTLRLKKRMIAEWIKNVASGYGKSIGEISYIFCS
                     DNKILAINNEYLQHDYYTDIITFDYTEEQKIAGDIFISVDTVSSNAHIYTNTFSDEMA
                     RILIHGILHLCGIKDKTQQEEQNMRKAEDKALALLKEKILR"
     gene            6440..8314
                     /gene="mnmG"
                     /locus_tag="PKN90_00120"
     CDS             6440..8314
                     /gene="mnmG"
                     /locus_tag="PKN90_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNZ84721.1"
                     /translation="MKFTYDIIVIGAGHAGCEAACAAANLGSQTLLITMDMNKIAQMS
                     CNPAIGGIAKGQIVREIDALGGFTGIVTDKTSIQFRMLNRSKGPAMWSPRSQCDRAKF
                     IETWRSVIENTANLYVWQDSAVSLVIENGAIAGVKTAMGVTFSCKAVVLTNGTFLNGL
                     LHIGKVQLNGGRVSEPASYGITEQLVAMGFSQGRMKTGTPVRIDARSVDFSQTTIQPG
                     ETDYHKFSYLDTPAHSLKQLPCYTLYTNEKVHQVLEKGLKDSPLYNGQIQSIGPRYCP
                     SIETKIVTFSDKTEHQLFLEPEGESTTEYYLNGFSSSLPIDIQLNALKEIPAFRNVHI
                     FRPGYAIEYDFFDPTQLRHTLETKLVKNLFFAGQINGTTGYEEAAGQGLIAGINAHMN
                     CVGGEKFILHRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADNRLTEKS
                     YTIGLATKERFELFKQKQTWCNEITEFIKNYSVKSSEIDSLLLSVGSTPLKQTTKLMD
                     ILLRPGISLQALIEALPSLQEQVEKITNRKQEIIEQIEISAKYEGYIARERLIADKLQ
                     RLENIKIRDKFNYDDISSLSTEAKQKLKRINPETIAQASRIPGISPSDINILLVLLGR
                     "
     gene            8396..8920
                     /locus_tag="PKN90_00125"
     CDS             8396..8920
                     /locus_tag="PKN90_00125"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNZ84722.1"
                     /translation="MTEEQVKKAIRDIPDFPSKGILFKDLTTAFKEPSCMKWFENELY
                     KRYKDKGITKIVGIESRGFVVAPILGNRLNIGFVPMRKKGKLPAETVEISYSKEYGID
                     TIELHKDSLTTDDIILIHDDLLATGGTMIAACKLAKKFNVKKIYVNFLVELDFLDGRK
                     GFDEDIEIDSLLHF"
     gene            8923..>9096
                     /locus_tag="PKN90_00130"
     CDS             8923..>9096
                     /locus_tag="PKN90_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNZ84723.1"
                     /translation="MYENLKHIVDTLPELSGVYQYLNQKEEIIYVGKAKNLRKRVSSY
                     FSKSLTSKKTAVLL"
BASE COUNT         2909 a         1683 c         1589 g         2915 t
ORIGIN      
        1 cctgaataaa acaatagcac aggatggttg ggaacggctt ctatcgcctc tttcgctatt
       61 tgataggctt tttcattttc atttttttct gtataatatt gtagtaaata tacccaacat
      121 tcgagacacg aaggatggag taaagctgct gtatagaaat gctctatcgc ctcagggact
      181 aatttttgtt gaagtaaaaa ttcagcatat gcagtatgag ctacatactc catggggtgc
      241 gactgcaata aagaagtata taaattatgc attttttcta ttccatccat tcttttgctg
      301 taatagctgg ttagtatttc taaataatgc attttatctt gaaacgaaat atctttcgct
      361 ccaaaaagtt tatataagta ctcttctgaa gtgctaaaat cctttatcgc ttcgtaatat
      421 cgagcccaag aaatgtatac atagccactt tctggttgaa tatcagtagc cttttgaaaa
      481 tatttttttg cattaccata tttttttctt tcgtattcta tatccccttt cagtatccat
      541 aaagcatgat cattgggcgt ttttttaagc attgcatcaa tttctttgtg tgcttgcttt
      601 atatttttca acaatacgta ttgtaccgct ttatcgtacg ttattgcttt gtttaaccct
      661 atacgttttt ccaacaaatc aagtttttgt attgattttt tgtgttgtcc attttgtgta
      721 tacaattgag ataactcata taaaacctca tctttatctg ggaataaccg ctgaatacgc
      781 tctaacactt ctatggcttt agaaaaatcg ccattgctac ggtagagcaa cgcaagtttt
      841 tcttgataga aatagttttg aggagctaac tctgctgcat ttttgatata cccaaacccc
      901 atatcatctg ctttttgttg caaaaacaaa ttgcctaatt caaaagaaac aatcgcacta
      961 gtaggattga tatcataact tttgagaaac aaatcaatac tttcggcagt atttcccaaa
     1021 tgcttttgtc gcaaagcttc tgcaaaataa taatcgaaat cgggctgttg agctgaccta
     1081 tcaaccactt gtgtggatag tttttcctga ttgttggtag ctattgctac aggcacaaca
     1141 agcgtaatga atataagagt tagtgaaaaa aatctcattc gtatcatcta ttttttacca
     1201 gtatgaccaa aaccgccagt accacgttgt gtttcatcaa gctgttctac ttgttgccaa
     1261 cgaacagttg cgtgctctgc cactaccatt tggcatatac gctcgccatc atttacaaca
     1321 aacggttcgt ccgaaatatt aaccaaaata acaccgattt caccgcgata atcggcatca
     1381 atcgtgccag gagtattgag cactgtaatg ccttttttga gagcaagtcc gcttcgtggt
     1441 cgaatttgag cttcgtaccc cttgggtagc tctatataca aacccgtagg aattaattta
     1501 cgttgcaaag gtagcaaagt aatgggctca gaaaggttag cacgtatatc cattccagca
     1561 gataagggcg tagcatactc gggtaaatcg tgttttgact tgtttacaat gttaatctgt
     1621 atcatttctt tatatattca aaatagttac ataatactta tgctaataca aaatctagct
     1681 gtttcttttc taaattggct ttggctacca gcacagccac ttcatcgcct aactggtaac
     1741 gcttgtgtaa gcgtgtgcct ataaggcaat aatttttttc gtcatatgtg taataatcat
     1801 cgtctaaatc gcgaatagga atcataccct cacacttatt agagataatt tccacataaa
     1861 tgccccattc ggtaatacct gaaataaccc catcaaatac ctgccctacc ttatctttca
     1921 taaactctac ttgcttgtac ttgatagaag ccctttctgc cgaagcagca agttgttcgc
     1981 gatctgaaca atgttcgcaa tactcttcgt acttatcagc aggagctgat tttccacctt
     2041 ccaaatagtc tgttagcaaa cggtgcacca tcatatcggg ataacgacgg atgggcgagg
     2101 tgaaatgaga ataatggtca aaagctaaac cgtaatgacc tatgttttta gtcgaataaa
     2161 tcgcttttgc catagaacgc acagccaatg tttcgattaa attttgctct ttttttcctg
     2221 ttacttcatc taataagtga ttgagcgaat tggcaatgtc ttttttctta cccgttgttt
     2281 tgagtttata accaaaacgc ttgatgaaaa gggagaaatt tgccagtttt tcttgatttg
     2341 gctcgtcgtg aatacgatac acaaatgttt tagctttttt tcctttgggt acatttccga
     2401 tatgctcggc taccgttttg ttggcaagca acataaattc ttctatcaat ttattggcat
     2461 ctttttgctc tttaaaatat acacttaatg gttttccttt ttcatctatt tcgaacctca
     2521 cctcgtatct atcaaaagca atagcaccaa gatcaaaacg tttttgtcga agttttttag
     2581 caagcgtatc caaagccaac aattctgaca caaactcccc ttgtttcttt tctattattt
     2641 cttgagcctc ttcgtatgta aaacgtttgt cggaacaaat aaccgttttt gcaatcttgt
     2701 atcgcacaat atctgcacta tcattcaact caaatatcac cgaataacac agcttgtctt
     2761 cgttggcacg taacgaacat acttcgttcg acagtttttc gggcaacata ggtatggttc
     2821 tatctactaa atagacagaa gtagctcgag caaacccctc cttgtcaata ggcgtatgtg
     2881 gtttaacata atgagttacg tcggcaatgt gtacgcccac ttctatattt ccattgtcta
     2941 aaatgcgaca cgaaagagcg tcgtcaaaat ctttggcatc gcgcgggtcg atggtaaagg
     3001 tggtaataga acgcatatca atgcgtttgg ctacttcttc gggcgtaatg cctgcgtcaa
     3061 tattttttgc catacgctct aattcttctg ggtaagaata aggtaaccca aattcagcta
     3121 atatggcgtg catttcggta ttattctcgc ctacttcgcc aagcacatcg ctaatttcac
     3181 caaacggact tttggcattg ggtgtctaat cggtcatttt taccaatact ttttgaccat
     3241 ctttagcccc attgagcttg tccattggta tataaatatc cgcatttaat atccgattgt
     3301 caataatcac aaaggcaaca ttatttttga tagacaacac gccaacaaaa gtttctctcg
     3361 accgtttaat tatttcaaca acttcgcctt ctggttgacg gtttttacga gcggcataaa
     3421 gcaatacttt gaccgtatca tttttcattg cacggtttac atttctatca gcaataaata
     3481 ttttctctcc gccatcttct ggaattaaat gtgtttttcc gttactattt ttatcaatta
     3541 ttcctattac aaacttactt ttagagctaa aacggaactt ccccctatcc atctcttcta
     3601 ccgaaccaag ctctgctaat tcgttaagca cttgttgtac attccgttta tccgtttggg
     3661 atttaacatt gatagcagaa gctaactgtt tgtaattaaa taacttatat ggagactctt
     3721 taaaaacctc catcatattt attgctatgt cttttttctt catacgtatt gttattatcg
     3781 tttgtcttta tcaagcaaaa cctttttctt tttttatcag ttcgtaagca ttttttacgg
     3841 ctttaaactt ttcggttgca atttctttaa actcatcgcc caaagtactt accttatcgg
     3901 gatgatactt catagccatg cgacgatagg ctttttttat ttcttcgtcc gaagcatttt
     3961 tatctatttc taagatttga tacgctaaat caagctcttt tcgtgtgtct tttacttgtc
     4021 catggaaatg ttgatgctga gagtatatcg cttgaataga ttgaaaatcg cccattgaaa
     4081 tacccattag tgtagccatt gtttgtaaaa ggcgtaattc ggacgattca attgtatcag
     4141 cagaagatat tttaaataaa atttgaagca attgcaattt ggatgaataa tttaaccctt
     4201 gatttatttg caaacaaatt gatttataat cataatcttg ttctaaaata tttttcaata
     4261 tttgcagagc ttctagtgct tcttcttcac caaaatttgc tcttaaaaaa gcttttgttt
     4321 cttgtaattc tgattttgtt acctttccgt cagccttcaa cacagtggct atcaaaataa
     4381 gaagagataa tttaaaatca ttttgagagg tgtatttttt ctttttttca ccataattag
     4441 aactaatagc cgagccaaga aatacaccaa taatggcacc gataggtcca ccaaccgtaa
     4501 accccaatgc tccacctaaa aaactaccca aaaatcccat ttatcgctct atttttgtta
     4561 atttacaaag gtattgattt tttgtcaact tatgatttaa aattaattaa aaacatggaa
     4621 gttttatgtt tatccgcaaa aagagaaata ctattcagtt aaattttaca tataaaaaag
     4681 caggaaagga gaagatttga aaaaattgct aaaaaatacg aaagcatgcg atatttttgt
     4741 aactttgtgg gttaaataat tcttaatatg gtattgaatt acatttggat aggatttttt
     4801 ctaattgcat ttgtagtggc attgttcaaa ttactagtgt tcggagatat gacaatcttt
     4861 ccaaacatta tgaattcgac gtttgaatat gccaaaatgg gttttgaatt atcacttgga
     4921 ctaaccggag taatgaccct ttggttggga gtgatgaaaa taggcgaaaa cggtggtgct
     4981 atcaagctat tatccaaagg cattagtccg ctatttaaca agctatttcc agaaattccg
     5041 aaaaatcacc ctgtgcatgg ctctatggta atgaatattg ctgccaacat gttggggtta
     5101 gacaatgccg ctaccccact tggtttaaaa gcaatgaacg aattgcaaga gttaaataaa
     5161 acgaaggata ccgcatccaa tccgatgatt atgtttttgg ttttgaacac atctggatta
     5221 acgattattc ccatttctat tatggtatac cgagcacaaa tgggagcagc agatccgtcg
     5281 gatattttta tacccatact tctttctact tttgtgagca cattggcggc tattatagcc
     5341 gtatccattg ctcaaaaaat aaacctattt accaaaacaa ttttacttta tttaggaagt
     5401 atcacacttt tgctaacagg agtgatttgg ggttttactg tattgagttc tgaaaaacta
     5461 aacatggtat cttctgtagc cgcaaacatt attttattta gcataattat tggatttatt
     5521 ttattggcaa tacgcaaaaa aataaatgtg tacgattcct ttattgaagg agctaaagat
     5581 gggttttcaa ttgccgttaa aattattccg tttttagtag ctatcttggt aggtataggc
     5641 gtgtttaggg cttcaggagc tatggacagt ttaatgtcgg gcattgcttg gctgtttaca
     5701 tcaatcggat taaatgcaaa ttttgtagaa gctttacctg ttgcctttat gaaacccttg
     5761 agtggagcag gagctcgtgg gctaatgata gatgcaatga accagcatgg tgtagattca
     5821 ttcgttggtc gtttggcatg tacgtttcaa ggggcaacgg atactacgtt ttatataatt
     5881 gcagtgtatt ttgggtcagt aggtatacga aaagttcgtc atgctgtaac atgcggatta
     5941 attgctgatt tagctggaat tattgctgct atttttattg catatttatt ctttcatacc
     6001 taattatcac tatggcagta cattttatat ttgatgatac taaaacgctt cgtttaaaaa
     6061 aaagaatgat agctgaatgg ataaagaatg tagcctctgg atatggaaaa tctattggtg
     6121 aaatcagcta tattttttgt tcagacaaca aaattcttgc tatcaataac gagtacctac
     6181 aacatgatta ctatacagat atcataacct ttgattacac tgaagaacaa aaaatagcag
     6241 gagatatatt tatcagtgta gatacagtaa gcagcaatgc acacatttac accaacacat
     6301 ttagcgatga aatggcacgc attttaatac atggcatttt gcatttgtgt ggcataaaag
     6361 acaagacaca acaagaagaa caaaacatgc gaaaggctga agataaagcc ttagccttat
     6421 tgaaagaaaa gatactacga tgaaatttac gtatgatata atagtgattg gtgctggtca
     6481 tgcaggatgt gaagctgcat gtgcggctgc aaaccttggt tcgcagacct tgctcataac
     6541 aatggatatg aacaaaattg cacaaatgag ttgcaatccc gccatcggag gaatagctaa
     6601 aggacagata gtgagggaaa tagatgcact tggaggcttt acaggcattg ttacagacaa
     6661 aacatccatc caatttagga tgcttaatcg ctccaaagga cctgctatgt ggagtcctcg
     6721 ttcgcaatgc gacagagcta aatttataga aacatggcga tcggttatcg agaatactgc
     6781 caatttgtat gtatggcaag attctgcggt gtcgttagta atagaaaatg gtgcaatagc
     6841 tggagtaaaa acagctatgg gagttacttt ttcgtgcaaa gcagttgttc taacaaatgg
     6901 tacattttta aatgggcttt tacatattgg caaagtacaa ctaaacggag gaagagtttc
     6961 agaaccagca agctatggta taacagaaca attggtagca atgggttttt cacaaggaag
     7021 gatgaaaacg ggtacaccag tacggataga cgcacgaagc gttgatttta gccaaacaac
     7081 tattcagcct ggcgaaacag attaccacaa attttcctat ctagatacgc ctgcacatag
     7141 cttaaaacaa ttgccttgct acactcttta taccaacgaa aaagtacacc aagtattaga
     7201 gaaaggacta aaagattccc ctctatataa tggacaaata caaagtatag gtccgcgtta
     7261 ttgccctagt attgaaacta aaattgtaac cttttctgac aaaacagaac accaactctt
     7321 tttagaacct gaaggtgaaa gtactacgga atattatttg aatggatttt cgtcatcctt
     7381 gcctatcgac attcaattaa atgctctcaa agaaatacca gcttttcgca atgtacatat
     7441 tttcagacct ggatatgcaa tagaatacga ttttttcgac cctacccaat taagacatac
     7501 cttagaaaca aaactggtaa aaaacctgtt ttttgcaggt caaataaatg gcacaacagg
     7561 gtacgaagaa gctgctggtc aggggttaat tgcaggaatt aatgcacata tgaattgtgt
     7621 tggtggagaa aaatttatct tacatagaga cgaagcatat attggtgttt taatagatga
     7681 tttggtaacc aaaggagttg acgaacctta tcgcatgttt acatcgcgtg ctgaataccg
     7741 tatcttgcta cgacaagacg atgcagacaa tcgtttaact gaaaaatcat ataccatagg
     7801 tttagccaca aaagaacgat ttgaattgtt taaacaaaaa caaacctggt gtaacgaaat
     7861 aacagaattt attaaaaact actctgttaa aagcagcgaa attgattcac tgctactctc
     7921 agttggctcc acgccattaa aacaaacaac aaaactaatg gatattctct tgcgaccagg
     7981 tattagccta caagcattaa ttgaagcatt accaagccta caagaacaag tagaaaaaat
     8041 aacaaataga aaacaagaaa ttattgagca aatagaaata tctgccaaat acgaaggata
     8101 tattgcaaga gagcgtttaa ttgctgataa gctacaacga ttagaaaaca taaagattag
     8161 agacaaattc aactacgacg acatcagctc tttatcgaca gaagcaaaac aaaaactaaa
     8221 aagaatcaac ccagaaacga tagcccaagc tagcagaata ccaggaatat ctcctagcga
     8281 tatcaatatt ttattagtgt tattaggaag ataaatttgt ttcacgtgaa acaaatttat
     8341 cttaaaagaa cacaatgttc cacgtgaaac atacaacaaa aaaaaacaaa agcatatgac
     8401 cgaagaacaa gtaaaaaaag caattagaga cattccagat tttccaagta aggggatatt
     8461 atttaaagac ctaactacag catttaaaga accatcctgc atgaaatggt ttgaaaatga
     8521 actatacaaa agatacaaag acaaaggaat caccaaaatc gttggtatag aatctcgtgg
     8581 cttcgttgtg gctccgatat taggaaatag gctcaatatc ggctttgttc ctatgcgtaa
     8641 aaaaggcaaa ctaccagcag aaacagtaga aataagctac tctaaagaat acggtatcga
     8701 taccatagaa ctccataaag attctcttac tacagacgat attatcttga ttcacgatga
     8761 cttgctagct actggtggca caatgattgc cgcatgcaaa ttagctaaga aatttaatgt
     8821 aaaaaaaata tacgtcaatt ttttagttga attggatttt cttgacggtc gtaagggatt
     8881 tgacgaagac atagaaatag attctctgct ccatttctaa ctatgtacga aaatctcaaa
     8941 catattgttg atacacttcc ggaactttcg ggagtgtatc aatacttgaa tcaaaaagaa
     9001 gaaataatat acgtagggaa ggcaaaaaac ttgcgaaaac gtgtctcttc gtatttttct
     9061 aaatcactta cctcaaaaaa aacagccgtt cttcta
//
LOCUS       DAOPUR010000004         8588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_8229_length_8588_cov_3.196765, whole genome shotgun sequence.
ACCESSION   DAOPUR010000004 DAOPUR010000000
VERSION     DAOPUR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8229_length_8588_cov_3.196765"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="PKN90_00135"
     CDS             complement(<1..114)
                     /locus_tag="PKN90_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ84724.1"
                     /translation="MKAMIFAAGLGTRLKPLTDSMPKALVPINGKPLLEHVL"
     gene            complement(127..1557)
                     /locus_tag="PKN90_00140"
     CDS             complement(127..1557)
                     /locus_tag="PKN90_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNZ84725.1"
                     /translation="MEETLLQLFLRVIGEQPTSVVALPIAGSNRKYLRMCTKKQSFIG
                     VVGTVLEENKAFITIANQFVSKGLPVPRVVGVSDDEMCYLVEDLGDVSLFDSIKKGRE
                     TGEFSAQEIELLKKTIAYLPSLQIEGANGFDFSICFPQAAFNYRTVMWDLNYFKYCFL
                     KPAGIECNEEWLENDFEHLASFLLQDKSNTFMYRDFQSRNVMIRNGNPYFIDFQGGRK
                     GPIQYDVASFVYQAKANFSAEVRETLIASYLEALSKYMPVDEVAFRKQLAFFVLFRNL
                     QVLGAYGFRGLVEKKKHFVESIPFAIQNLNEILKNNYFDTHFPYLVSVLQQLIVRYTH
                     QDKPTSPSGLVVKVSSFSYKKGIPEDTTGNGGGYVFDCRAIHNPGKYEQYKQLTGLDK
                     PVIDFLEQDGEILAFLEHIYALADSSVSRYVERGFSSIMFSFGCTGGQHRSVYAAQHV
                     AEYIATRYSVKVELVHREQGIKQQFN"
     gene            complement(1566..2048)
                     /locus_tag="PKN90_00145"
     CDS             complement(1566..2048)
                     /locus_tag="PKN90_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84726.1"
                     /translation="MARLLLFLIPLLLVSCVAPADYNDSLIREQRRVLSAINDLNTVI
                     KQANKEETNSELAEAVNQVEKSILKVQQKDAFRGETQLREEMEELLVFYKFVLNEYYP
                     LLIEIQHDEGLTLTEKKQVSDSIYDTIREQEIQRTTAYAEAQKLFADKYNISIIHTQK
                     "
     gene            complement(2048..2818)
                     /locus_tag="PKN90_00150"
     CDS             complement(2048..2818)
                     /locus_tag="PKN90_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84727.1"
                     /translation="MKKLILLALGFLVATCMGVAQVGVPVVKSTNAPNKDKVNVSTIT
                     FNYPAKDIEIAVLEKLKKEGLTPRKSKSKFVVFAGVEYFPLWDNKFDFYLRISGSNNA
                     GTVELLMTQGYNNYINPTTPEVQERVNKWLLGLEKTVSDYIYERAMAEHLKEKKLIER
                     TLSSLQTQQDRLTNQYDNLNKQQEAFEKTKVDVSKADPNTLNTKDIAKQQKQQEKIVK
                     ERNKLEYSLYDVRSRIAASQKDLDNRNKAIDELKQQKR"
     gene            complement(2867..5308)
                     /locus_tag="PKN90_00155"
     CDS             complement(2867..5308)
                     /locus_tag="PKN90_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ84728.1"
                     /translation="MKYKYVLSLLWLFFVVFIIRAQDGKIIGQVFDNTSKKPLDFANV
                     ALFTNQSKEPLQAILSDSEGKFILQNLPYGSFTLKVSFVGYESFETSVLVSKEKPTAR
                     VNKVVLKENAQQISEVQVVGQRSGVSFEIDKKVFNVDETALAQGSSTTDVLKNIPSVA
                     VDLEGNVSLRNNSSVEIWINGKPSGLTDENRGQVLEQMPAETVEKIEVITNPSARFNP
                     EGSAGIINIVLKEKNRGSYLASINSNIGYDESGAINGSFGGNYMYNGPKFDVQLNGSI
                     RGGERKNASYNNRATLVAEDTISHLNQRSLSESDMFMGFMRGGVTYHIDKKNDIGLSG
                     FGMLGDFSGNSTINYHSLDANRDTLETRDRLADNANNRMHYNVSLDYKHFFDKTNHEL
                     SSSIYYVRGNHNSFNAYNTSRFGNDGVYINDSKTYQEQQATRNMYNITWQADYFNKFT
                     KDSKLESGLSFNYRNSLSEDRMYDSIFTTNSFVEDVDKYNRFTYVENIFSAYTTYSQK
                     IQTFSFQLGLRAEQTMTEAKQHYRSYFSLFPSVFLSQSLPQNNELQINYTRRINRPRG
                     WQLNDFVNRTDELNLSYGNPSLRPEFTNSFEFNYIKNWEQGHTFSTSLYYRATSDVIQ
                     RVSKLQNEIMETTYENITTSESAGAELILKNRLFANKLDLTSTLNAYYYALAANESYG
                     ISSVENFTWNARMNANIIIQRGFTTQLTAYYRAPQVKVQGRDEQVYGIDLGARKTFFK
                     GALNVGVSVRDLLDSQRTTNITWGENFYQESASLYSGRSYRLTLTYNFGNMKNGGKEQ
                     RNKNNNGFSEENLEF"
     gene            complement(5385..5798)
                     /gene="aroQ"
                     /locus_tag="PKN90_00160"
     CDS             complement(5385..5798)
                     /gene="aroQ"
                     /locus_tag="PKN90_00160"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNZ84729.1"
                     /translation="MQLVIINGPNLNLLGKREPQVYGNQTFEEMLAEIRKAFPSIDIL
                     YFQSNVEGEIINKIHEVGFTADGIIINAGAYTHTSIAIADAVKSIKTPVVEVHISNVF
                     ARESYRHESFLTPVCKGCISGFGIKSYYLAIQSFF"
     gene            complement(5840..6859)
                     /locus_tag="PKN90_00165"
     CDS             complement(5840..6859)
                     /locus_tag="PKN90_00165"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNZ84730.1"
                     /translation="MEEINWSNLSFSYMPTDCNIRCSYQNGEWGELRKHTDVYLNMHM
                     AATCLHYGQECFEGLKAFRGKDGKIRVFRIDENALRMQSSCEGILMAKFPVDKFKEAV
                     KLVVKENERFVPPYESGASLYIRPLIIGTSAQVGVNAAKEYEFILFVTPVGPYFKGGF
                     QTTPFVILRQFDRTAPLGTGMYKVGGNYAASLVAVDKAHKMGYSNVFYLDAKEKKYID
                     ECGAANFFGIKNNTYITPKSTSILPSITNKSLMVLAKELGLKVEQRPVPEEELATFEE
                     AGACGTAAVISPIQRIDDVEEGKSYVFSADGKPGKVCEQLYTKLRAIQYGDEPDKYGW
                     ITVIE"
     gene            7082..>8588
                     /gene="sppA"
                     /locus_tag="PKN90_00170"
     CDS             7082..>8588
                     /gene="sppA"
                     /locus_tag="PKN90_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNZ84731.1"
                     /translation="MKQFLKFTFASCLGFLLSFFTICIIVAIVIVATVTGFSSKQITV
                     APNSVLKIEFNGVLKEQARDMSFFDAWSSTNITELGIHDIIKSIQHAKTDENIVGIWL
                     QMDLFSAGNASISEIRDALVDFKKSGKFIIAYGDNYTQQLYYLATVADKIFIHPSGTI
                     ELKGLASKPIFFKNALQKVGVDMQVVKVGDYKGAPEMYTNNAMSDENKAQTKNIINQL
                     WRKMCTNIASERNISIEQIESLVEKSVMFAPTKSYVTEGLVDSLLYPDELVSYFKTHI
                     DSTYNEEKQMVSYGDYIVLASQNKSNNNKIAVLYANGGIDDGDIEGIDSEELSKLLSE
                     TRKDSSIKAVVLRINSPGGSAFGAERIWRETSLFKGVKPLVVSMGDYAASGGYYIATQ
                     ADSIFSNTETLTGSIGIFGIIPNFSKISEKFGITYDPQTTHTLADFPSFTRDMTTYER
                     NILERYITDNYQLFLHRCSEGRSITKAALLPLAGGRVWTGSDALTHGLVDKI"
BASE COUNT         2675 a         1677 c         1392 g         2844 t
ORIGIN      
        1 caaaacatgc tcaagtagcg gttttccatt aatgggtaca agagctttgg gcatactatc
       61 agtaagcggt ttcaaccgtg tacccaatcc tgctgcaaaa atcatcgctt tcatacgtat
      121 ggtattctaa ttaaactgtt gtttaatgcc ttgttcacga tgtactaatt ccacttttac
      181 agaatagcgt gtagctatat attcggctac atgttgtgct gcatatacag agcgatgttg
      241 tcctccggtg caaccaaaag aaaacataat tgaagaaaaa cctctttcta catacctgct
      301 taccgaacta tcagctaatg cataaatatg ctcaagaaag gctaaaattt cgccatcttg
      361 ttccaaaaaa tcaatcacgg gtttgtctaa tcctgttagt tgcttatatt gctcgtactt
      421 accaggatta tgaatagccc tacaatcaaa gacgtaacca cctccgttgc ccgttgtatc
      481 ttcgggtata ccttttttat acgaaaaact acttaccttt accactaagc ccgatgggct
      541 ggttggctta tcttggtgtg tatagcgaac gattaattgc tgtaatacgc taacgagata
      601 cggaaaatga gtatcaaaat aattattttt gaggatttca tttaaattct gaatagcaaa
      661 agggatgctt tctacaaaat gctttttctt ctccactaat cccctaaaac cgtaggctcc
      721 aagtacttgc aaattcctaa ataacacaaa aaatgcaagt tgtttgcgaa aagccacctc
      781 atctacaggc atatacttcg acaatgcctc caaatagctt gcaatcaagg tttcacgaac
      841 ttctgccgaa aaattggctt tggcttgata cacaaacgaa gctacatcgt attgaattgg
      901 accttttcgt cctccctgaa aatcaataaa ataaggattt ccgttgcgta tcatcacatt
      961 gcgactctga aaatcgcgat acataaacgt gttactttta tcttgaagca agaatgatgc
     1021 cagatgttca aaatcatttt ctaaccattc ttcgttacat tcgataccag caggttttaa
     1081 aaaacaatat ttaaagtaat tcaaatccca catcaccgta cggtaattaa aggctgcttg
     1141 cggaaaacag atggaaaaat cgaaaccatt tgccccttca atttgcaacg acggtaaata
     1201 agcaattgtt ttttttagta gctcaatttc ttgtgcacta aactctcctg tttctctgcc
     1261 tttcttaatc gaatcgaata acgatacatc acccaaatct tcaacaagat agcacatctc
     1321 atcgtctgac acacccacta cacgtggcac gggtaagcct ttggaaacaa actgattagc
     1381 aatggtaata aacgctttat tttcttcgag cacagtacct actacaccta taaacgactg
     1441 ttttttggtg cacatacgta ggtatttcct gttagaacca gcgatgggca atgctacaac
     1501 ggatgtaggc tgttcgccta ttacacgcaa aaaaagttgt aacaaggttt cctccatggt
     1561 agtttctatt tctgagtatg aatgatggat atattgtact tgtcggcaaa cagtttttga
     1621 gcctctgcat aagcagttgt acgttgaatt tcttgttcac gtatggtatc gtaaatagaa
     1681 tcggaaactt gctttttttc agttagcgtt aagccttcat cgtgttgaat ttctatcagc
     1741 aatggataat actcgtttaa aacaaactta taaaaaacaa gcaactcctc catttcctct
     1801 cgcaactgtg tttcccctct aaaagcatct ttctgttgca ctttaaggat agatttttct
     1861 acctgattga cagcctctgc tagttcacta ttggtttctt ctttgttggc ttgtttgata
     1921 actgtattca agtcattaat agcagacagc accctacgct gttcgcgtat caacgaatcg
     1981 ttataatcag caggtgctac acacgacacc aacaacaacg gaataagaaa taatagcaat
     2041 cttgccatta gcgtttctgt tgtttcagtt cgtcaattgc tttgtttcta ttgtctaaat
     2101 ctttttgcga agctgcaatt ctcgaacgta cgtcatacaa gctatattct agcttattac
     2161 gttctttcac aattttctct tgctgttttt gctgtttggc tatatctttg gtattcaagg
     2221 tatttggatc tgctttagag acatctactt tggttttttc aaacgcttct tgttgtttat
     2281 ttaaattatc gtattgattt gttaatctat cttgttgcgt ttgtaaagag gataaggtac
     2341 gctcaatcaa ctttttctct ttcaaatgct ctgccatagc tcgctcataa atataatcac
     2401 tcacggtttt ttccaatcca agcaaccatt tattcaccct ttcttgtact tctggggtag
     2461 taggattgat ataattatta tacccttgtg tcattaataa ttcaacggta cctgcattgt
     2521 tagagcctga aatacgaaga taaaaatcga atttattatc ccaaaggggg aagtattcta
     2581 ctccagcaaa aacgacaaat ttcgatttag attttcgtgg tgttagacct tcttttttta
     2641 gtttttctaa taccgctatt tcaatatctt tagctggata attgaatgta atagttgaaa
     2701 cgtttacttt atctttattc ggagcatttg ttgatttaac aaccggcaca ccaacctgtg
     2761 ctactcccat gcaagtagct actaaaaaac ccaatgctag taaaataagc tttttcatac
     2821 cttagttttt ttcaattaat agtacacaaa gataaagcaa tattttttaa aattcaaggt
     2881 tttcttcact aaaaccattg ttgtttttgt tacgctgttc tttgcctcca tttttcatgt
     2941 tgccaaaatt atacgtaagg gtgagcctat aacttcttcc tgaatagaga cttgcacttt
     3001 cttgataaaa gttttcgccc catgtaatgt tggtagttcg ttgagaatca agcaaatctc
     3061 tgacagatac tcccacattg agagcccctt taaagaatgt ttttctagct cctaaatcaa
     3121 taccatacac ttgctcatca cgaccctgca cttttacttg tggagcacga taatacgctg
     3181 ttagctgtgt agtaaaacct cgttgaataa taatattcgc attcattctt gcattccaag
     3241 taaaattttc taccgatgaa atgccataac tttcattcgc agctaacgca tagtagtagg
     3301 cattgagtgt agaggttaaa tctaatttat tagcaaataa cctgtttttt aaaatcaact
     3361 cggctcctgc tgattctgat gttgtaatat tttcgtaagt agtttccata atttcgtttt
     3421 gcaacttgct tacacgctga ataacatcag aagtagcacg ataatacaat gaggtagaaa
     3481 acgtatgacc ttgctcccag ttcttaatat aattaaattc aaatgaatta gtaaactcgg
     3541 gacgcaaact aggatttccg tatgacagat ttaattcgtc agtacgatta acaaaatcat
     3601 tgagttgcca acctcgtgga cgattaatac gtcgcgtata atttatctgc aattcgttgt
     3661 tctgtggcaa agattgtgat agaaagaccg aaggaaataa actaaaataa gacctataat
     3721 gctgctttgc ctcagtcatt gtttgttcag cacgcaagcc aagttgaaaa ctaaatgttt
     3781 gtattttttg agagtaagtg gtatatgctg aaaaaatatt ttccacataa gtaaatcgat
     3841 tgtacttgtc tacatcttcg acaaaagaat tggtagtaaa gatggaatca tacattctat
     3901 cttcgctcaa cgaattgcga taattaaagc ttaagccact ttcgagttta gagtctttgg
     3961 taaatttatt aaagtaatca gcctgccaag taatgttata catattgcgt gtagcttgtt
     4021 gctcttgata tgtttttgaa tcgttgatat acactccatc gttaccaaaa cgggacgtat
     4081 tgtaggcatt aaagctatta tgattacctc gcacataata aatagacgac gacaactcgt
     4141 ggttagtttt atcaaaaaaa tgcttgtaat caagagatac attgtaatgc atccgattat
     4201 ttgcattatc agctaatctg tccctcgttt caagggtatc tctatttgca tctaatgaat
     4261 gataattaat tgtgctattc cccgaaaaat ctcccaacat accaaaacca ctaagtccaa
     4321 tatcattttt tttgtcgata tgataggtta cgcctcctct cataaagccc atgaacatat
     4381 ctgactcaga taacgatcgt tgattaagat gcgagatagt atcttctgct accaaagttg
     4441 cccgattgtt gtatgatgca tttttacgtt ctcctcctcg tatagaacca ttcaattgca
     4501 catcaaattt gggaccgttg tacatataat taccaccaaa agaaccattt atagcccccg
     4561 actcatcata accaatattg ctattaatac tagctaaata ggagccacga tttttttctt
     4621 tcaatacaat attaataatt cctgcacttc cctctggatt aaaacgggct gatggattgg
     4681 ttataacctc tattttttct accgtttcgg ctggcatttg ctctagcacc tgccctctat
     4741 tttcatctgt caaaccagat ggcttgccat ttatccaaat ttctactgac gaattatttc
     4801 gcaacgaaac atttccttct aaatcaaccg ctaccgaagg aatatttttt agcacatcag
     4861 ttgtggaaga accttgtgca agtgcagttt catccacatt aaatactttt ttatctattt
     4921 caaaagacac tcccgagcgt tgtccaacta cctgtacctc tgatatttgt tgtgcatttt
     4981 ctttcaatac aactttattt accctagctg taggtttctc tttgctcacc aacacagagg
     5041 tttcaaaaga ttcatagcca acaaaagaaa ctttcaatgt aaacgaccca tagggtaaat
     5101 tctgaagaat aaacttacct tcactatcag acaatattgc ttgtaatggc tcttttgatt
     5161 gattagtaaa tagagctaca ttggcaaaat caagcggttt tttagacgta ttatcaaata
     5221 cttgaccaat aatttttccg tcttgagccc gtataataaa cacgacaaaa aaaagccata
     5281 gtagacttaa cacatattta tatttcatat tcgttacgtt taggtgttgt gcctctatga
     5341 cggaaaaaag agaaaaaggt ttaaagcgga aatgttattt tatgtcaaaa aaaactctgt
     5401 atggctaaat aataggattt aattccaaat ccagaaatac agcctttgca aacgggagtt
     5461 aaaaaagact cgtgtcggta tgattcgcga gcaaaaacat tagatatatg tacttctacg
     5521 acaggagttt taatggattt tacagcatcg gcaatagcaa tagaagtatg tgtataggct
     5581 cctgcattga taataatacc atcagcagta aaccctactt catgaatttt attaataatt
     5641 tcgccttcta cgttagattg aaaatacaga atatcaattg atggaaatgc ttttcggatt
     5701 tccgcaagca tttcctcaaa tgtttgattt ccataaactt gcggctctct tttgccaagc
     5761 aaatttaaat taggaccatt tataataact aattgcatag cgtaagtttc aatgtaaaga
     5821 cagttggttg ttcgtttttt tactcaataa ctgttatcca accatattta tctggctcat
     5881 ctccgtattg aatagcacgt aatttagtat ataattgttc gcatactttc cctggtttac
     5941 catctgcaga aaacacatat gactttcctt cctctacatc atcaattcgc tgaataggac
     6001 ttattacagc tgcagttccg catgctccag cttcttcaaa agtagccaat tcttcttcag
     6061 gaactggacg ttgctctacc tttaaaccca attcttttgc caaaaccatt aaacttttgt
     6121 tggttattga tggtaaaatt gacgttgatt ttggtgttat gtacgtattg tttttaatcc
     6181 caaaaaagtt tgctgcacca cattcatcaa tgtatttttt ttcttttgca tctaaataaa
     6241 atacatttga ataccccatt ttatgagctt tatctacagc aaccaagctt gctgcataat
     6301 taccacccac tttgtacata cctgttccta aaggagctgt acgatcaaac tgccgtaata
     6361 tcacaaacgg agtagtttga aaacctccct taaaatatgg accaacaggc gttacaaaca
     6421 aaataaactc atattccttt gctgcattca caccaacctg agcactggta cctataatta
     6481 acggacgtat gtataacgag gctccacttt cgtaaggtgg cacaaagcgt tcattttctt
     6541 ttacaaccaa tttaaccgcc tctttgaatt tgtcaactgg aaattttgcc atcagtatac
     6601 cttcacacga cgattgcata cgaagtgcat tttcatcaat cctaaacaca cgaatcttac
     6661 catcttttcc tctaaaagct ttcagtcctt cgaaacattc ttgcccgtaa tgcaaacaag
     6721 tagcagccat gtgcatattc aaatacacat ctgtatgttt tcgtaattct ccccactctc
     6781 cgttttgata tgaacaccga atattacaat ccgttggcat ataactaaac gacaaattcg
     6841 accagttaat ttcttccatc tgcgaaattc taaaaatgtt tatagctatg tgtaataaat
     6901 atataagtgc aaaagtaatg tttttttagc aaatacaagt attgtatgtt tggtttttat
     6961 agaagttttt tttactacat tttaatttga cacaaaacta ttttatttta atctttaaat
     7021 cgctatcttt gtagtttatg atttacttta cgtataattt tgtacatatt ttatattcaa
     7081 gatgaaacaa tttctgaaat ttacttttgc ttcttgcttg ggcttcttac tttctttttt
     7141 tacaatatgc attatagttg caatagttat tgttgctact gttactggat tttcatctaa
     7201 acaaataacg gttgcaccta attctgtatt aaaaattgaa tttaacggcg ttttgaaaga
     7261 gcaagcaaga gatatgtcct tttttgatgc gtggtcatcc acaaatataa ccgaacttgg
     7321 tattcacgat ataataaaga gcattcagca tgccaaaaca gacgaaaata ttgttggaat
     7381 ttggttgcaa atggatttat ttagtgctgg aaatgcctct ataagcgaaa ttcgagatgc
     7441 attagtcgat tttaaaaaat caggtaaatt tattattgct tatggggata attacaccca
     7501 acaattatat tatctagcaa cggttgctga taagattttt atacatccat cgggaacaat
     7561 tgaactcaaa ggattggctt caaaaccaat tttctttaaa aatgcactcc aaaaagtagg
     7621 cgttgatatg caggttgtaa aagtgggcga ttataaagga gcacctgaaa tgtatacaaa
     7681 taatgctatg agtgatgaga ataaagctca aacaaaaaac attatcaacc aactctggcg
     7741 aaaaatgtgt acaaatattg catccgaaag aaatataagc attgaacaaa tagaatcctt
     7801 ggttgaaaaa tcggtaatgt ttgccccaac aaagtcgtac gttacagaag ggcttgtaga
     7861 tagcttacta tacccagacg aacttgtttc ctatttcaaa acccacattg atagcacgta
     7921 taacgaagaa aaacaaatgg ttagctatgg agactacata gttcttgcat cacaaaacaa
     7981 atcaaataac aataaaattg ccgtactata tgctaatggg ggaattgatg atggcgatat
     8041 tgaaggaatt gattctgaag aactgtctaa attgctatca gaaactcgaa aggattcttc
     8101 tataaaagca gtagtacttc gcatcaattc gccaggaggc agtgcttttg gagcagaacg
     8161 catttggaga gaaacatctc tttttaaagg ggtgaaaccc ttagtcgtat caatgggcga
     8221 ttatgctgct tcaggaggct attatattgc aactcaagct gattctattt tcagtaatac
     8281 cgaaacgctt actggatcca ttggtatttt tggtattatc ccaaacttct caaaaatttc
     8341 tgagaaattt gggattactt acgaccctca aacaacccat acattagcgg actttccttc
     8401 ttttacaaga gacatgacca catatgaacg taacatcttg gaaagatata ttacagataa
     8461 ttatcagttg tttttacaca gatgttctga gggtcgatct atcacaaaag cagcattact
     8521 accactagct ggaggcagag tatggacagg atcggacgct ttaacacacg gcttagtaga
     8581 caaaatag
//
LOCUS       DAOPUR010000005         7376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_10049_length_7376_cov_3.827346, whole genome shotgun sequence.
ACCESSION   DAOPUR010000005 DAOPUR010000000
VERSION     DAOPUR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10049_length_7376_cov_3.827346"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1527
                     /gene="tig"
                     /locus_tag="PKN90_00175"
     CDS             175..1527
                     /gene="tig"
                     /locus_tag="PKN90_00175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNZ84732.1"
                     /translation="MNVSQNNIDQVNAVLSIQVKKADYTEKVEKSLKDYRKKANMPGF
                     RPGTVPLGLIKKMYGKAILAEEINKLVSESLYDHIQKNNIPVLGEPLPSENAPVPNFD
                     TQEDFDFSFDIALAPEINLNLTTKNKVTLYNIAVSDEMVAKQVDAYKGRFGKHAPADK
                     VEEKDVVKGELVELLPNGDINENGIKVENALLSPAYMKDKKEQAKVIGLALESQFRFN
                     PAKAFESETEIASLLQVSKEIAADCKADFLFTIKDITRYVEAEMNQDLFDAALGKDVV
                     SSEAEFIDKIKESLKAQFAVDSEFKLSIDMKELVVKQLEGLAFPDVFLKRWLKVSNEN
                     LTEDAIEQEYPKMIEDLKWQLAKDKVAKENELKLEKEDVEAVAKQAAKAQFAQYGMMN
                     VPDDVLDGYVKDMLKNKDAAKNITERAVEQKTIDALKTKVTIVSKEISFEDFNKLFEN
                     "
     gene            1674..2342
                     /gene="clpP"
                     /locus_tag="PKN90_00180"
     CDS             1674..2342
                     /gene="clpP"
                     /locus_tag="PKN90_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNZ84733.1"
                     /translation="MNTEFKKYATKHLGMNGLALDKYVDITSSYISPTIIEERQLNVA
                     QMDVFSRLMMDRIIFLGTQVDDYTANVIQAQLLYLDSADPGKDISIYLNTPGGSVYAG
                     LGIYDTMQFISSNVATICTGMAASMGAVLLTAGTAGKRSALKHSRIMIHQPMGGAQGQ
                     ASDIEITAREIQKLKKELYTIIAEHSGNTYERIEQDSDRDYWMTAEEAKAYGMIDEVL
                     VKQK"
     gene            2363..3583
                     /gene="clpX"
                     /locus_tag="PKN90_00185"
     CDS             2363..3583
                     /gene="clpX"
                     /locus_tag="PKN90_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNZ84734.1"
                     /translation="MSKKDDKCSFCGRGRQEVPLLMQGQTGNICNDCAEQAATIAKET
                     FSKQSDKYQLDKQTLPKPQELKAFVDQYVIGQDDAKKNLCVAVYNHYKRLLQKNTHDD
                     VEIEKSNIILVGATGTGKTLLARTIAKKLHVPFTIVDATVLTEAGYVGEDIESILTRL
                     LQVADYDVKAAEKGIVFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSVVNVP
                     PQGGRKHPDQKMIAVNTQNILFICGGAFDGIEKKIAARLNTQIVGYAAAKITHQIDKD
                     NLLQYISPQDLKAFGLIPEIIGRLPILTYLNPLDREALRLILTEPKNSIIKQYKKLFA
                     MDDVVLEFDKDVLDYIVDKSIEYKLGARGLRSIAEMIMVDYMYDIPSSNEHQLRIPLA
                     IAKEKVEKGRMTPV"
     gene            3689..5878
                     /gene="recQ"
                     /locus_tag="PKN90_00190"
     CDS             3689..5878
                     /gene="recQ"
                     /locus_tag="PKN90_00190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNZ84735.1"
                     /translation="MSTSLELTQELKKNFGFDSFKGNQEAIIKNLLAGNDTFVLMPTG
                     GGKSLCYQLPSLMMEGTAIVISPLIALMKNQVDAMKAFSEEDGVAHFLNSSLSKAAIE
                     DVKNDVISGKTKLLYVAPESLTKEENILFLKKVKISFYAVDEAHCISEWGHDFRPEYR
                     RIRPLINEIGSAPVVALTATATPKVQHDIQKNLGMVNATVFKSSFNRENLYYEVRTKN
                     QHIDKEIIKYIKSQHGKSGIIYCLSRKKVEKLAETLCLNGINALPYHAGMESAVRTAN
                     QDSFLMEKVDVIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEGQC
                     ITFYSNKDLQKLEKFMQGKPISEQEIGKQLLLETAAYAESSVCRRTLLLHYFGEEYTQ
                     DNCGSCDNCLNPKKQMEAQDLLCTVLETILSVKEKFKAEHIVDVIVGKETAEVIDYKH
                     QDLEVYGTGESEKPTVWHAVIRQGMLSGYIDKEIENYGLLKVTKSGRAFLEKPTSFKI
                     TKDNDFEEEDDDDATIRRSGGGTGAADEVLFSMLKDLRKKMSKRLEVPPFVIFQDPSL
                     EAMATTYPVTMEELQNIPGVGAGKAKRYGEDFLRLIKTHVQENEIERPVDLRVRTVAN
                     KSKLKVFIIQAIDRKIALDDIAISKGLEFPELLDEIEAIIYSGTKINIDYFINEVMEE
                     DHILDIYEYFRESQTDEVETALAELGSDYTEEEIRLIRIKFVSEMGN"
     gene            complement(6020..>7376)
                     /locus_tag="PKN90_00195"
     CDS             complement(6020..>7376)
                     /locus_tag="PKN90_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ84736.1"
                     /translation="FKTSNYSKITTEYHHSTEYRRGGDLLDLPAHEANLAEQLQHNID
                     AGSVKFDLYTKDNKHFISTYSAFQHIVRNSYFGTGQDLDAYGKSKDINSVTGAQYRYS
                     MDNLLFMPADFSMGIEYTYNQLQDQMLGHGIDVNQKVSIFGGYLQNEWKNNQLSLLAG
                     ARVEKHNLMNNPVVSPRVSLRYNPTHNIVLRTGYASGYRAPQIYNEDLHVAAVGGEVS
                     IISQDANLRPEYSHSFSVSADAYHRFGFWETNLTVDGFYTKLNDVFALEENGFDSKGN
                     LLLLRVNESGATVTGVNIEGVLGYKNLLSLQLGYTLQSSLYDEPVTWSTNTAIEATRR
                     MQRTPDHYGYAVMTYNATKNFTASVSGKYTGSMLVPHFAGYIAEDTETISSPFWDMGV
                     KLAYKIPFYKYYALEVNGGVKNILDSFQPDLDKGMDKDAGYIYGPTLPRTFFVGLSLK
                     L"
BASE COUNT         2521 a         1157 c         1474 g         2224 t
ORIGIN      
        1 gctgtggcgt aattggtagc cgcgccagac ttaggatctg gtgccgagag gcgtgggggt
       61 tcgagtccct tcagccgcac aacaaaaaag aaccgttgtc ttagcaaaca caacggtttt
      121 ttttgcctat aacaaaaagt attttgctga ataataaatt aatttcgtag tacaatgaat
      181 gtttcacaaa acaacatcga ccaagtaaat gccgtacttt caattcaagt aaaaaaagcg
      241 gattatactg aaaaagtaga aaaatcatta aaagattatc gtaaaaaagc aaatatgcct
      301 gggtttagac caggaactgt gcctcttgga ttaatcaaaa agatgtacgg aaaagctatt
      361 ttggcagaag aaattaataa acttgtttca gaatcgctat acgatcatat ccaaaagaat
      421 aatatacctg ttttgggaga gcctcttcca tcggaaaacg cccctgtgcc aaattttgac
      481 acacaagagg atttcgattt ttcttttgat attgctctgg ctcctgaaat caaccttaat
      541 ttaacaacca aaaataaggt aacattatac aatattgctg taagtgacga aatggttgcc
      601 aaacaagtag atgcttacaa aggacgtttt ggaaaacacg ctccagcgga taaggtagaa
      661 gaaaaagatg tagtaaaagg cgaattagtt gagttattgc ctaatggcga tatcaacgaa
      721 aatggtatta aagtagaaaa tgctctatta agtcctgcgt atatgaaaga caaaaaagag
      781 caagctaaag ttattggatt agctcttgag tcgcaattta ggtttaatcc agctaaagca
      841 tttgaaagcg aaacagaaat agcctcttta ttgcaagtta gtaaagaaat tgctgccgat
      901 tgcaaagctg atttcttgtt taccatcaaa gatattacac gctatgtcga ggctgaaatg
      961 aatcaagatt tatttgatgc agctcttgga aaagatgtgg tatcttctga ggctgaattt
     1021 attgataaaa ttaaagaaag cttaaaagct caatttgctg ttgatagtga gtttaaactg
     1081 tctattgaca tgaaggagtt ggtggttaaa caacttgaag gcttagcttt tcctgatgta
     1141 ttcttaaaaa gatggttgaa agtgagcaac gaaaacctga ccgaagacgc tattgaacaa
     1201 gaatatccta aaatgataga agatttgaaa tggcaattag caaaggataa agtggcgaaa
     1261 gaaaacgaat tgaaattaga aaaagaagac gtagaagcag tagccaaaca agcagctaaa
     1321 gctcaatttg ctcaatatgg catgatgaat gtgcctgatg atgtacttga tggctatgta
     1381 aaagatatgc ttaaaaacaa agatgctgca aaaaatatta ccgaacgtgc cgtagaacaa
     1441 aaaacaattg atgccctaaa aacaaaagta acaattgttt caaaagaaat atcattcgaa
     1501 gattttaaca aactgtttga aaattaagca tataggatat aaaaaatact aaaaaaactt
     1561 tcttttaaat ttgggagtgt aatttgtttg ttttaaaaca aaaaaaaatt atacctttgt
     1621 ttccctgatt taaaagaaag tttttttatg taccatataa tttggaaaac aatatgaata
     1681 cagaatttaa aaaatatgca acaaagcatc tcggaatgaa cggattggct ttggataaat
     1741 atgtggatat tacaagtagc tatatatcgc caactattat tgaagaaaga caactcaatg
     1801 tcgcacaaat ggatgttttt tcacgtttga tgatggacag aattatcttt ttaggaacac
     1861 aagtggacga ttatactgcc aatgtaattc aggctcaatt gttgtatctt gattctgccg
     1921 atccagggaa agatatttct atttatctta atacacctgg aggttctgtg tatgcaggct
     1981 taggtatata cgataccatg cagtttattt ctagcaatgt agctaccatt tgtacaggca
     2041 tggcagcttc tatgggtgct gtactactta cggctggtac tgcaggcaaa cgttcagcat
     2101 taaagcactc ccgtattatg attcaccaac caatgggtgg agctcaaggt caagcttcgg
     2161 atattgaaat tactgcccgt gaaattcaaa aactcaaaaa agaattatac accattattg
     2221 ccgaacattc gggcaataca tatgaacgta tcgaacagga ctctgaccgt gactattgga
     2281 tgacggcaga agaagcaaaa gcctatggta tgattgacga agtgttggta aaacaaaaat
     2341 aagagcaagt aggtataaag ttatgtcaaa gaaagacgat aaatgtagtt tttgtggacg
     2401 aggacgtcag gaagtgcctc tactgatgca aggacaaaca ggaaatatat gcaacgattg
     2461 tgccgaacaa gctgctacca ttgccaaaga gactttttct aaacaatcgg ataagtatca
     2521 actcgataaa caaaccttgc ctaaaccaca agaattaaag gcatttgttg atcagtatgt
     2581 aattggacaa gatgatgcaa aaaagaatct atgtgtagcc gtttacaatc actataaacg
     2641 attgttacaa aaaaacacac atgatgatgt agagattgaa aaatcgaaca tcattttagt
     2701 tggagctaca ggcacaggaa aaaccttgtt agcacgcact attgctaaaa aactgcatgt
     2761 cccttttact attgtagatg caactgtttt aaccgaagca gggtatgttg gtgaagatat
     2821 cgaaagtatc ttaactcgcc tattgcaagt agctgactat gatgtgaaag ctgctgaaaa
     2881 aggtatcgta tttattgacg aaatagataa aattgctcgc aaaggagaca atccttctat
     2941 tactcgcgat gtgagtggtg agggcgtaca acaaggatta ctaaagctcc ttgaagggtc
     3001 tgttgtaaac gtacctcctc aaggaggacg aaaacatcct gaccaaaaaa tgattgccgt
     3061 aaatactcaa aatattttat ttatttgtgg gggagcattt gatggtattg agaaaaaaat
     3121 tgcagcacga ttaaatactc aaatagtggg atatgcggct gcaaaaataa cgcatcaaat
     3181 agataaagat aatttacttc aatatatttc accacaagac ttaaaagctt ttggattgat
     3241 acccgaaata attggaagat tacctatttt aacgtatctc aatccattag atagagaagc
     3301 attgagatta atattaaccg aaccaaagaa ttctattatc aagcaatata aaaaattgtt
     3361 tgctatggat gatgttgtat tagagtttga caaagatgtt cttgattata ttgtagataa
     3421 atctattgaa tataagctgg gtgctagagg tttacgctct atcgctgaga tgattatggt
     3481 agattatatg tatgatatac cttcatcaaa tgaacatcaa ttacgcattc ctttggcaat
     3541 tgccaaagag aaagtggaga aaggaagaat gacaccagtg taatttattc aaaatttttc
     3601 tcgctttcat ttgattatta aaaacaagtt attacatttg ttttattata caaacaagag
     3661 caactagttt taatcttaca ttattgtaat gtcaacatct ttagaactta cacaagaatt
     3721 aaaaaagaat tttggattcg attcttttaa agggaatcaa gaagccatta ttaaaaatct
     3781 gttagcaggt aatgatacat ttgtattgat gcctactggg ggaggtaagt ctttgtgtta
     3841 ccagttaccg tcgcttatga tggaaggaac agctatcgtt atttctccac ttatcgcttt
     3901 gatgaaaaat caagtagatg caatgaaagc atttagcgaa gaagatggcg tggctcattt
     3961 tttaaattca tcactatcga aagcagctat cgaagatgtg aaaaatgatg ttatttctgg
     4021 caaaacgaaa ttgttgtatg tagcccctga atctcttacc aaagaagaaa atattttatt
     4081 tctgaaaaaa gtaaaaattt ctttttatgc agttgatgag gctcattgta tttcggaatg
     4141 ggggcacgat tttagaccag aataccgcag aattcgtccg ttaattaatg aaataggttc
     4201 agctccagtg gtagctctta ctgcaacggc tacacccaaa gtgcaacatg atatacaaaa
     4261 aaatttagga atggtaaatg ctactgtttt taaatcgtct tttaatagag aaaatttgta
     4321 ttatgaggta cgcacaaaga atcagcatat tgataaagaa attattaaat atatcaaatc
     4381 tcaacatggt aagtcgggta ttatttattg tttaagtcgt aagaaagtag aaaaattagc
     4441 tgaaactctc tgtttaaatg gaataaatgc cttgccatac catgcgggaa tggaatctgc
     4501 tgttcgtaca gccaatcaag atagtttttt gatggaaaaa gttgatgtaa ttgtggcaac
     4561 aattgcattt ggaatgggta ttgataagcc cgatgttcgt tttgtaatac attatgatat
     4621 acccaaaagc ctcgaaggtt attaccaaga aacaggtaga gctggtagag atggaggtga
     4681 aggtcaatgc attacgtttt actcaaataa agacttgcag aaattagaaa aatttatgca
     4741 aggaaaacct atatcagaac aagaaattgg caaacaactc ttgttagaaa cggcagctta
     4801 tgctgagtcg tctgtatgta gaaggacatt attgttacat tatttcggag aagaatacac
     4861 acaagacaat tgtggtagtt gtgataattg tttgaaccct aagaagcaaa tggaagcaca
     4921 ggatttatta tgtacagttt tggaaacgat cctttcagtg aaagaaaaat ttaaggctga
     4981 gcatattgta gatgtaatcg ttgggaaaga aaccgcggaa gtgattgatt ataaacacca
     5041 agatttggaa gtatatggta caggcgaaag cgaaaagccg actgtttggc atgctgttat
     5101 acgacaaggt atgttatcag gctatatcga caaagagata gaaaattatg gattgttgaa
     5161 ggttaccaaa agtggtcgtg cttttttaga aaaacctaca agctttaaga taacaaaaga
     5221 taatgatttt gaggaagaag atgatgatga tgcaaccata agaaggtcgg gtggaggcac
     5281 tggagctgct gatgaggtat tgttttctat gctcaaagac ttacgaaaga agatgtcgaa
     5341 gagattggaa gttccgcctt ttgtcatttt ccaagaccct tcgttggagg caatggctac
     5401 aacgtatcca gttactatgg aagagttgca gaatatacct ggagtaggtg caggtaaagc
     5461 caaacgctat ggagaagatt ttttgcgttt gattaaaaca catgtgcaag aaaatgaaat
     5521 tgaacgccct gttgatttac gggtgcgtac agtggctaat aaatctaaac taaaagtctt
     5581 tatcattcaa gctattgaca gaaaaattgc attagatgat atcgctatat ccaaggggtt
     5641 agagtttcct gagttgttgg atgagattga agccatcatt tattcgggta ctaaaatcaa
     5701 tatcgattat tttatcaacg aggtaatgga ggaagatcat atcttggata tatacgagta
     5761 ttttagagag tctcaaacag acgaagtgga aacagctctt gccgaattgg gaagcgatta
     5821 tacagaagaa gaaattcgat tgatacgcat aaagtttgtg tctgaaatgg gcaattaact
     5881 tcttgcagaa ataagatttt taaagagata gcgtttattt gttatctctt ttttttgtaa
     5941 aaaatgggac aacgaaaagt tgtcccacaa aagaaaagtt gtcccacaaa agaaaaggct
     6001 cactacttca ttttcttttt tatagtttta gacttagtcc tacgaaaaat gttcgaggca
     6061 atgtaggtcc gtaaatatat ccagcatctt tatccatacc tttatctaag tcaggctgaa
     6121 agctatccag tatatttttt acacctccgt ttacttcaag tgcatagtat ttgtaaaaag
     6181 gtattttgta tgctagttta acccccatat cccaaaatgg agatgaaatt gtttcagtat
     6241 cttcggctat gtaacctgca aaatgtggta ctagcatact tcctgtatat ttaccagata
     6301 ctgatgctgt aaagttttta gttgcgttat aagtcataac agcgtatcca taatggtctg
     6361 gtgtgcgttg catgcgtctt gtggcttcga tagcagtatt ggttgaccag gttactggtt
     6421 catcatataa gctactttgt aaggtatagc ctagttgtag tgatagtaag tttttatatc
     6481 ctaacactcc ttcgatattt actcccgtta cggttgctcc cgattcatta acccgcaaca
     6541 ataataagtt tcctttgcta tcaaacccat tttcttctag tgcaaataca tcattcagtt
     6601 ttgtatagaa gccatctacc gttaagttgg tttcccaaaa accaaaacga tggtatgcat
     6661 ctgctgatac agagaaacta tgcgaatatt caggacgaag gtttgcgtct tgcgagataa
     6721 tagacacttc tccacccaca gccgctacgt gtaaatcttc gttgtatatt tgtggagcac
     6781 ggtatccgct agcgtatcca gttcttagta caatgttgtg tgttgggttg tagcgtaaac
     6841 ttacacgggg cgatactact ggattgttca ttaaattgtg tttttctaca cgagcacctg
     6901 ccaataggct tagttgatta tttttccact cattttgaag gtatccacca aagatgctaa
     6961 ctttttggtt tacatcaatt ccgtgcccta acatttggtc ttgaagttga ttatatgtat
     7021 attcaattcc catactaaaa tctgctggca taaacaatag attatccatc gaatagcggt
     7081 attgggctcc tgttacagaa ttaatatcct ttgattttcc ataggcgtca aggtcttgac
     7141 ctgttccaaa ataactatta cgcacaatat gttgaaatgc actataggta gaaataaagt
     7201 gtttgttatc tttggtatac aaatcaaatt taacgctccc tgcatctatg ttatgttgaa
     7261 gttgctctgc taaattggct tcatgggctg gtaaatcgag caagtcgcct cctcttctgt
     7321 attcagttga atggtggtat tcggtagtaa tttttgaata attactagtt ttaaag
//
LOCUS       DAOPUR010000006         7155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_10468_length_7155_cov_3.288310, whole genome shotgun sequence.
ACCESSION   DAOPUR010000006 DAOPUR010000000
VERSION     DAOPUR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10468_length_7155_cov_3.288310"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKN90_00200"
     CDS             complement(<1..351)
                     /locus_tag="PKN90_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNZ84737.1"
                     /translation="MQEDSYQSILQRAEGIYKEKGSKFLSFAIPVCSVDDAKMELQSF
                     RANYHDARHICYAYVIGYPNSVVRSSDDGEPSGTAGKPILAQIQAKQLLDVMVVVVRY
                     FGGVLLGTSGLITAY"
     gene            464..1702
                     /locus_tag="PKN90_00205"
     CDS             464..1702
                     /locus_tag="PKN90_00205"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNZ84738.1"
                     /translation="MVLVNENYLKLSENFFFTEIEQRINAFNLIHPNTHVIRLGLGDV
                     CLPLPDEIVSAMENAAKEMGKIETFRGYGPETGYRFLIDKIIKHDYNSRGISLEANEV
                     FVSDGSKADLGNIGHIFGRDNIIAITDPVYPVYENAAIMGGRSGYLTEDNIWSNIVYI
                     PCTEENNFTPNLPTEKVDIIYLCFPNNPTGVALDKTELKKWVDYAIENQTIIIYDGAY
                     CSYITQKDVPRSIYEIKGAKKVAIEIRSFSKTAGFTGLRCSYTIVPRELMGYTLMGEK
                     VDINKLWRRRATTYYNGTSYIIQRGAEASYSLKGKKQLQKNIDYYMKNAAIIRKELLE
                     LGFHVYGGINSPHIWIKTPKNTPSWKFFQQLLYESHIVCTPGAGFGQFGEGYFRFSGF
                     NTRENTIEAMERMQVWRNRI"
     gene            1831..4215
                     /locus_tag="PKN90_00210"
     CDS             1831..4215
                     /locus_tag="PKN90_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ84739.1"
                     /translation="MKSKLSGIIIVSMLLCINNYVQADNVQIRGEVVDAFSGHAIIGA
                     TVIIENSNPIIGCISDYDGNFVLSNLQPGRYTINVSSVGYHPASIHNMMAISGKDNVL
                     VFKLEEKINHLKDIIVTANNNKFDAINEFAFTSARSFTVEETERFAGSWNDPSRMVSN
                     FAGVMSSNDARNDIIIRGNSPSGLLWNIDGVNVVNPNHFGSQGTTGGPISMLNSNLLA
                     NSDFITGAFPSEYGNALSGVFDLNLRSGNRERFEFMGQIGLNGFEGLVEGPLKLGKNL
                     QNGSFIVDYRYSTLKVLRDIGFDFGTGDAIPKYQDLAVLIDLPTKNSGRFKFTGITGK
                     SNIEIGRNFDEQEALGKGPMNSASDFSGNVLIGILSHTYMCNEKTRIKSSISFQESGN
                     EIINDTIDYINKTYFNYYAANQNEKKVTASFQIKHRVNVQNNFTVGLIGNQLITSYHD
                     SVWVNEYAIRKYLRNIDKKNSSLVQAYATWQHKFSENLIVNSGLHSQYYDLNQQLSFE
                     PRISGLWKFSPNQQISFGYGLHSQLQPRIIYFTKEYDYTTNMYGNDNQHIGFSRAHHY
                     VMGYDIRIGRNFRIKTEGYYQNLFDIPISSQNVQYSMLNTGSSYYYEPVYGLENSGKG
                     RNYGVELTVEQFMHKGLYFLLTGSLFDSKYKAYDQIWRNTAFNTNYASNLLAGYEMRV
                     GKKHFLTFDIRAVWSGGLRYTPIDLNMSILTGEEVISVDKTNEEQFPDYIRCDFRTEF
                     KYNSKRITQETGIDLNNVTNHQNIFSQHYNKATQQISTVYQQGFMLILFYRINL"
     gene            4277..5314
                     /locus_tag="PKN90_00215"
     CDS             4277..5314
                     /locus_tag="PKN90_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNZ84740.1"
                     /translation="MEPNRKGFLSHFIIYSRWYTVLILTSIISIIVGFVISYFLDFRL
                     TGWHYYLRVLATTLVLWIGCSTIAIYIWKKYPWILMPFKHLMMEVIGVFCLLTLYFGF
                     TLLIYMYSHVGISLIQSIEIHSIAFIIITFTTFFILAIHEAILFYNQWHEHYSKSIRL
                     EKDTIEAQYNLLKTQINPHFLFNSLTSLMSMVDGNPKAEKYIQTLSDYLRYMLADNIN
                     ELITLKDEVNNVTKFFYLQKIRFENNIHLKIRIDDVALNKKIVPLVLQILLENCIKHN
                     IIANKMPLYIEIYNDENYVSIKNNLQIKTSKQSIGKGIKNIEGRYQFLSANAIKISKT
                     NETFTVSVPLL"
     gene            5326..6084
                     /locus_tag="PKN90_00220"
     CDS             5326..6084
                     /locus_tag="PKN90_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNZ84741.1"
                     /translation="MLKILIIEDEKPALALLEKMLAQTINNFTIIGRCDSIESSVTFL
                     KKHQDIDLIFMDIHLGDGNSFDIFLQIQIDTPIIFTTAFDMYAIKAFELNSIDYLLKP
                     IKKEDLTRSIEKFYKSKKHLQQFDIQYLSSIISNRSNIYKERFLVYIGNNIKAIMTSD
                     IAYFYSLDSATYLVTHNGNTYDVNMSLEQLIGVLDPQIFFRINRQYIVAFSAIDKIQL
                     LSTYRIKIVLLPPTDKEILVSSNKNQLFKEWLNR"
     gene            6181..>7155
                     /locus_tag="PKN90_00225"
     CDS             6181..>7155
                     /locus_tag="PKN90_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNZ84742.1"
                     /translation="MDFELVSPYKPTGDQPQAIEQLTSGILGGISDQTLLGVTGSGKT
                     FTIANVIEQVNKPTLVLSHNKTLAAQLYREFKSFFPNNAVEYFVSYYDYYQPEAYLPT
                     TDTYIEKDLAINDEIERLRLSTTSSLLSGRQDIIVVSSVSCIYGIGNPDDFTNNVINI
                     KQGEQIVRDVFLRQLVSSMYNRNEIDFQRGNFRVKGDTVDIWLAYADIMLRVIFWGNE
                     IESITTHDPLHLFTLEHHDAYKIFPATIFVTTKDSIHRAIGEIEIDLGKHVDYMKSIG
                     KHYEAKRLYERVTYDLEMIRELGYCSGIENYSRYFDGRAPGSRPFCLLD"
BASE COUNT         2491 a         1133 c         1223 g         2308 t
ORIGIN      
        1 atacgccgtt attaaaccgc tagtacctaa tagtacaccg ccaaaataac gcaccacaac
       61 aaccataaca tccaacaatt gcttggcttg tatctgagct aaaatgggtt ttcctgctgt
      121 gccagatggt tctccatcat cgctactacg cacaacggaa tttggataac caataacata
      181 cgcatagcaa atatgtcgtg catcgtggta gtttgccctg aaagactgca attccatttt
      241 tgcatcatct actgagcaaa caggaatggc aaaagacaaa aacttgcttc ctttttcttt
      301 gtaaataccc tcggctcttt gtaaaatact ctggtaacta tcttcttgca tacggttata
      361 cctaaatcaa tacacaaaaa tacactttta agacaaaaaa agatgtgttt ttgattttgt
      421 ttttttatct tcgcagtatg ttagctgaaa taatcagaaa atcatggtat tagtcaacga
      481 aaattatcta aaactttccg aaaacttctt ttttacagag attgaacaac gtattaacgc
      541 attcaatcta attcatccta atacacatgt tattcggtta ggcttaggtg atgtctgttt
      601 gcccctaccc gatgaaatag tttctgccat ggaaaatgct gccaaggaaa tgggtaagat
      661 agaaactttc agaggatatg gtcctgaaac aggataccgc tttttaattg acaagattat
      721 aaaacacgac tataattcgc gaggcatttc gctcgaagca aacgaagttt ttgttagcga
      781 tgggtctaag gctgatttag gaaatattgg gcatattttt ggaagagata atattattgc
      841 cattaccgat cctgtatatc cagtgtacga aaatgctgcc attatgggag gtcgttcagg
      901 atacttaacc gaagataata tttggagcaa cattgtatac atcccatgta ccgaagaaaa
      961 taattttaca cccaacttgc ccacagagaa agtagatatt atctatttgt gttttcccaa
     1021 taacccaacg ggagtagctt tggataaaac tgaactaaaa aaatgggtgg attacgccat
     1081 tgaaaatcaa actattataa tttatgacgg tgcatactgc tcgtatatta cccaaaaaga
     1141 tgtgccacgt tcaatttacg aaataaaagg agcaaaaaaa gtggctatcg aaatacgcag
     1201 tttttctaaa acagctggtt ttactggatt acgttgctca tatacaatag tgcccagaga
     1261 gcttatggga tataccctta tgggagaaaa agttgatatc aacaaactat ggcgtaggcg
     1321 tgctaccacc tattacaatg gcacatcata cattatacaa cgtggagctg aagcttctta
     1381 ttcgttaaaa gggaaaaaac agttacaaaa gaatattgac tattatatga aaaacgctgc
     1441 tatcattcgt aaagaactat tggagcttgg ttttcatgta tatggaggaa ttaattcccc
     1501 tcatatttgg attaaaacac caaaaaatac accatcatgg aaatttttcc agcaactgct
     1561 ttacgaatcg catattgttt gcacaccagg agcaggattt ggtcagtttg gcgaagggta
     1621 tttccgcttt tccggattca atacccgcga aaacaccatc gaagcaatgg aaagaatgca
     1681 ggtgtggaga aataggatat aacacgaaag tatgttttca atttcaattt ttcaatattc
     1741 aatatccttt tttgtacata caactctttt tatttttaat caataaaacc ttaaaatacc
     1801 tttgcgttca ttaactatta taaatgtaca atgaaaagca aactatcggg tattattatt
     1861 gtgagtatgt tattgtgtat aaacaattat gtacaagccg acaatgttca aatcagagga
     1921 gaagtagtag atgcctttag cggacatgct attataggag caacagtgat tattgagaat
     1981 tctaatccaa taattggttg tatttctgat tatgatggca attttgtgtt atctaactta
     2041 caaccaggaa gatacaccat taatgtatct tctgtgggtt atcatcccgc cagcattcat
     2101 aatatgatgg caattagtgg aaaagataat gttcttgtat ttaagcttga agaaaaaatt
     2161 aatcacctta aggatattat tgttacagcc aataataaca aatttgatgc aattaatgaa
     2221 tttgcattta caagtgcacg gtcatttact gtagaagaaa ctgaaagatt tgctggcagt
     2281 tggaatgatc cgagtagaat ggtgtctaat tttgcaggtg tgatgagcag taatgatgcc
     2341 cgtaatgata ttatcataag aggaaactca ccaagcggat tattatggaa tatagatggt
     2401 gtaaatgttg taaatcctaa tcattttggt tctcaaggaa ctactggagg accaataagt
     2461 atgcttaaca gcaatctttt ggcaaactcc gattttatta cgggtgcttt tcctagtgaa
     2521 tatggtaatg cgttatcggg ggtgtttgat ttaaatctga gatcaggaaa tagagaaaga
     2581 tttgaattta tgggacaaat tggcttgaat ggttttgaag ggttagttga aggtccgtta
     2641 aaacttggaa agaacttaca aaatggctca tttatagtag attatcgtta ttctacatta
     2701 aaagttttac gcgatattgg tttcgatttt ggcacaggtg atgccattcc gaagtatcaa
     2761 gatttagcag tattaattga cctcccaaca aaaaattctg gacgttttaa gtttacagga
     2821 attaccggga aaagcaatat cgaaatagga agaaactttg atgaacaaga agcattggga
     2881 aaaggtccta tgaattcagc gtcagatttt tctggaaatg ttctgattgg aattttatca
     2941 cacacatata tgtgtaacga aaaaacaaga attaaatctt ctatatcttt tcaggaaagt
     3001 ggcaatgaaa ttataaatga tacgatagat tacatcaaca aaacctattt taattactat
     3061 gccgccaatc agaatgagaa aaaagttact gcatcttttc aaataaaaca cagggtaaat
     3121 gttcaaaata attttacggt tgggttaatt ggcaatcaac ttattacctc ataccatgat
     3181 tctgtatggg taaatgagta tgcaatacgg aaatatctaa gaaatattga caaaaaaaat
     3241 agttcacttg tgcaggctta tgcaacgtgg caacacaagt tctctgaaaa tttgattgta
     3301 aattctggtt tgcatagtca atattacgac ctcaaccaac agctaagttt tgaacctcga
     3361 ataagtggat tatggaaatt ttctcctaac caacaaatta gttttggata tggattacat
     3421 agtcaattgc aacccagaat tatatatttc acaaaagaat acgattatac tacgaatatg
     3481 tacggaaatg ataatcaaca tattggattt tctcgtgctc atcattatgt aatgggatat
     3541 gatatacgca ttggtagaaa tttcagaata aaaaccgagg ggtattatca aaacttattt
     3601 gatattccaa tttcttcgca aaatgtacaa tattcgatgc ttaatacagg tagtagctat
     3661 tattatgaac ccgtatatgg tttagaaaat tcgggtaaag gcagaaatta tggtgtagag
     3721 ttaactgtag aacagtttat gcacaaagga ctatattttc tacttacagg ttcgctgttt
     3781 gactcaaaat ataaagctta tgaccaaata tggcgtaata cagcttttaa tacaaattat
     3841 gcaagcaatt tgctggctgg ttatgaaatg cgtgttggta aaaaacattt tcttaccttc
     3901 gatattcgcg ctgtttggtc gggaggactg cgatatacac caattgactt aaacatgtca
     3961 atattaactg gtgaagaagt aataagtgtt gacaaaacga atgaagaaca atttccagat
     4021 tacataagat gcgattttcg aaccgaattt aaatacaact ctaaacgaat tacgcaagaa
     4081 actggcattg atttaaataa cgttacaaac caccaaaata tattttcaca acattataat
     4141 aaagctactc aacaaatttc aacagtatat caacaaggat ttatgcttat tttgttttat
     4201 cgtattaatt tataagcaca ttttcaattg gtagtgccat aaattttatg taatttcgca
     4261 caaaactact atgaatatgg aacccaatag aaaaggtttt ttatcccatt ttattatcta
     4321 ttcaagatgg tacacggtat tgatattgac aagtatcatt agtataattg ttggttttgt
     4381 tatttcatat tttttagatt ttcgtcttac tggatggcat tattacctac gtgtattggc
     4441 tacaaccttg gtattgtgga ttggatgttc tactattgca atatatattt ggaaaaaata
     4501 cccatggatt ttgatgccat ttaaacattt gatgatggag gtaataggcg ttttttgtct
     4561 attaacatta tactttggat ttacattatt gatttatatg tattcacatg taggtattag
     4621 tttaattcaa agcattgaaa tacattccat tgcatttatt attataactt ttaccacttt
     4681 tttcattttg gctatacatg aagctatatt gttttacaac caatggcacg aacattattc
     4741 aaaatcaatt cgactagaaa aagatactat tgaggctcaa tacaatttac tcaaaactca
     4801 aattaatcca cattttttat tcaatagcct tactagccta atgagcatgg ttgatggcaa
     4861 ccctaaagcc gaaaagtata tacaaacttt atctgattat ctgagatata tgctggcaga
     4921 caatataaat gaattaataa cactaaaaga tgaggtgaat aacgtaacta aattctttta
     4981 tttacaaaaa atccgatttg aaaacaacat ccatcttaaa attaggattg atgatgtggc
     5041 cttaaacaaa aaaattgtac ctttggtatt acaaatattg cttgaaaatt gcattaaaca
     5101 taatatcatt gcaaataaaa tgcctttata tatagagatt tataatgatg agaactatgt
     5161 aagcattaaa aataatttgc aaattaaaac aagcaaacaa tccattggta agggtattaa
     5221 aaatattgaa ggacgctacc aatttttatc agctaatgcc ataaaaatat caaaaacaaa
     5281 tgaaacgttt acagtatctg tgccattgtt ataagaaata tgtgtatgtt gaaaatatta
     5341 atcattgaag atgaaaaacc cgccttggca ttgttagaaa aaatgctggc tcaaacaatc
     5401 aataatttta caattattgg aagatgtgat agcattgagt cgagcgtaac tttcttgaag
     5461 aagcaccaag atattgattt aatttttatg gatatacacc tgggagatgg caatagtttt
     5521 gatatattct tacaaataca gattgataca cctatcattt ttactactgc ttttgatatg
     5581 tatgcaataa aagcatttga actaaacagc attgattatt tgttaaaacc cataaaaaaa
     5641 gaagatttaa ctagatcaat tgagaaattt tataagtcaa aaaaacattt acaacaattc
     5701 gatattcaat atttatcttc cattatttct aatcgttcta atatttataa agaacggttt
     5761 ttggtgtata tcggaaataa tataaaagca attatgacaa gtgatattgc ctatttttat
     5821 tcacttgaca gtgccacata ccttgtaaca cacaatggca atacttatga tgtaaatatg
     5881 tcgttggaac aattgattgg tgttttagac ccacaaatct tttttagaat taacagacaa
     5941 tacattgttg cattcagtgc tatcgacaag atacaattac tttctaccta ccgaattaaa
     6001 atagtattat tgccacccac agataaagaa atacttgtaa gtagcaataa aaatcagttg
     6061 tttaaagaat ggttaaatag atgaaaaata gtttttataa ctgaaaaaat tgttatctat
     6121 ctattcttat atttgcttgt atcaatataa gtcattacat tcgcgtaaat aatcatatct
     6181 atggatttcg aactcgtttc tccttataaa ccaactggcg accaacctca agctatcgaa
     6241 caattaacaa gtggcatttt gggtggaata agcgaccaaa ccttattagg ggttactggt
     6301 tcgggtaaaa catttaccat agccaatgtg attgagcaag taaataaacc tactctcgtt
     6361 ttaagtcaca ataaaacttt agctgctcaa ttatacagag aatttaaaag tttttttccg
     6421 aacaatgcag ttgagtactt tgtttcgtac tacgattact accagccaga agcatactta
     6481 ccgaccactg atacctatat agaaaaagat ttagccataa atgatgaaat agagcgtctt
     6541 aggctctcta ctacttcatc tctactttct ggcagacagg atattattgt tgtttcttcc
     6601 gtttcgtgta tctatggtat tggtaatcct gatgacttta cgaataatgt aattaacatc
     6661 aaacaaggag aacaaatagt gcgtgatgtg tttctcagac aattagtatc atccatgtat
     6721 aaccgaaatg aaattgactt tcagcgggga aattttaggg taaaaggaga tactgtcgac
     6781 atttggcttg cgtatgctga cattatgtta cgtgtcattt tctggggaaa cgaaatagaa
     6841 agcattacta cccacgaccc attgcactta ttcacactag agcatcacga cgcttataaa
     6901 atatttcctg ctactatttt tgttactacc aaagatagta ttcatagagc tattggcgaa
     6961 atagaaattg atttggggaa acatgttgat tatatgaaat caattggcaa gcattacgaa
     7021 gccaaacgat tatacgaacg tgtaacttac gacttagaaa tgatacggga gttaggctac
     7081 tgctctggca tcgaaaatta ttctcgttat tttgatggac gtgctcccgg atcaagacca
     7141 ttttgtttgc tcgat
//
LOCUS       DAOPUR010000007         7063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_10631_length_7063_cov_3.615725, whole genome shotgun sequence.
ACCESSION   DAOPUR010000007 DAOPUR010000000
VERSION     DAOPUR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10631_length_7063_cov_3.615725"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..810
                     /locus_tag="PKN90_00230"
     CDS             52..810
                     /locus_tag="PKN90_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84743.1"
                     /translation="MKENIVTLLYFLGIMGAILMYFVFPNDITHAILFGAICLIITII
                     PALLLSRTKKKKKPIYTYTDESFADFHPIQSDSLSMIEEVSNTTRKVSDSSLTAKRMY
                     GKVLAYVADFEELNKAPLSAEGRTECVLFVSTVVLLEYKSLYQIEEYRDFEVAYLDIM
                     LSLVHQNATQKSKEEVYEFVKRRLRFYTAEYQTLIDNKFPLEFYSYFFYTPLRTRTQV
                     CLDQNELIRFHGNLLSLVEKIKLSTKNDASFLGV"
     gene            complement(799..1473)
                     /locus_tag="PKN90_00235"
     CDS             complement(799..1473)
                     /locus_tag="PKN90_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ84744.1"
                     /translation="MNLIETQGVCKSFGTLQVLKNIDLTIRKGEFASIIGASGAGKTT
                     LLQIIGTLDKPTAGTITMNKVKLESLHSDELSELRNKEIGFVFQFHQLLPEFTALENV
                     MIPALIAKVQKKEAEKKAQELLHYLGLTNRLLHKPNELSGGEKQRVAVARALINNPSI
                     ILADEPSGSLDSRNKEELHQLLVTLNRDLGITLLVVTHDTNLAYLSNRIIEMQDGIII
                     GEKLNA"
     gene            complement(1470..2183)
                     /locus_tag="PKN90_00240"
     CDS             complement(1470..2183)
                     /locus_tag="PKN90_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNZ84745.1"
                     /translation="MWTSRTKLLLGENNSSILEKKHVMIVGVGGVGAYAAEMICRAGI
                     GNITIIDADTIKESNINRQLPALHSTIGKKKVTVLTERLLDINPNLVLKQHDVFIEKE
                     LIPSLFENLHVDFVIDAIDTLSPKIDLIRYCIQHKIKIVSSMGAGGRTDPSKIHYADI
                     SGTYHCTLAKHVRKRLKLVNISKGLPVVFSSEQVNQNSVIQIDDEKNKCSTVGTISYL
                     PAIFGCYLAAYVIKKLIQK"
     gene            complement(2191..3516)
                     /locus_tag="PKN90_00245"
     CDS             complement(2191..3516)
                     /locus_tag="PKN90_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNZ84746.1"
                     /translation="MQWETLLSTKRFGMEAYHKKDTEDRSEFQRDYDRLIFSSPFRRL
                     QNKTQVFPLPGSVFVHNRLTHSLEVACVGRSLGTNIAHQLSILHQKSSNFLPEIGSIV
                     SAACLAHDLGNPPFGHSGEKAIASFFSEGNGSFIKERLENETEWNDLIFFEGNANSLR
                     LLTHQFKGRRYGGFAMTYSTLASIVKYPYSSVECGKKGKFGFFESEKATYEQIAMELG
                     IIKSPVSDKKYARHPLVYIVEAADDICYQIMDIEDAHKLKILSTEQTKELLLQFFDTE
                     RKKRIYETWKLVDDSNEQISYLRSSVIGKLVSECARVFVENEQQILSGQFSGSLIDHI
                     EPVCKNAYENCSTIAVKKIYKSQEVLDIEVAGYKIIQTLLDEYIKASFQPNKSYSKLL
                     LDRVPSQYDMYAPSTYEKIQAIVDFISGMTDIYALDLYRKITGMNLPSL"
     gene            complement(3544..4266)
                     /locus_tag="PKN90_00250"
     CDS             complement(3544..4266)
                     /locus_tag="PKN90_00250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNZ84747.1"
                     /translation="MTQTYIHKDLGKILIYYSPKARSIRVQVGDNGIKLIIPLGVNID
                     KGLHFVNAKQDWIEKALKRTEQKKRHVSIHITELNSQTFSILTKACERTNILFRLHEK
                     ILTIEHPISLDITQAKYQQILLDGMEKFVVSEAKKHFSQRLAYLANKGGFTYNEFRIQ
                     RSKTRWGSCSSKKNINLCAYLLFVPAYLSDYVIIHELCHTKEMNHGNTFWNLVASQEP
                     NYITYRKEIKQYAFLMQLFNRK"
     gene            4414..4884
                     /gene="rimP"
                     /locus_tag="PKN90_00255"
     CDS             4414..4884
                     /gene="rimP"
                     /locus_tag="PKN90_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HNZ84748.1"
                     /translation="MISKDVVVQAVNQFLKDKEDYFLVDVTISIHNTICVEIDAFQGV
                     DVETCCELSRFIETKIDREIEDFELEVGSAGITAPFKILQQYIKNINNEVEVLLKNGK
                     KIIGFLRSANEHTFVVEVSQKIKKDKNAKKEEVTELLTYAYSDVKHTKYNLRFK"
     gene            4890..6143
                     /gene="nusA"
                     /locus_tag="PKN90_00260"
     CDS             4890..6143
                     /gene="nusA"
                     /locus_tag="PKN90_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNZ84749.1"
                     /translation="MSNKPVQSLNLIETFSEFKELKNIDKATMISVLEDSFRSVIAKM
                     FGTDESFTVVINPDKGDCEIWRDYEVVADGEVEDEVLQMGLSKARAIDDECEIGDDVT
                     EAVNFLSFGRRAVLTLRQTLASKILELQKESVYDQYKEMVGQVVYGEVYQIWKKEILL
                     LDDQGNELLLPKTEQIPTDFYKKGDTVKSVVVRVDNKNNQLKIIVSRTAPIFLQRLFE
                     QEIPEVQEGTIMIRRIARIPGERAKVAVESYDDRIDPVGSCVGVKGSRIHSIVRELRN
                     ENIDVINYTSNLSLFIARSLSPAKVLSIRIDEERKRAEVSIKPEDVSMAIGKGGSNIK
                     LATMLTEYTIEVFRHVDEEDEDDVFLDAFSDEIEPWVIDVFKSIGCDTAKSVLEIPRQ
                     ELIDKTDLEEETIDEALAILRQEFE"
     gene            6228..>7063
                     /locus_tag="PKN90_00265"
     CDS             6228..>7063
                     /locus_tag="PKN90_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNZ84750.1"
                     /translation="MSTKLNKVVKELNVGLATAVEFLHKKGFADVESNPNARITEEQY
                     DLLVKEFSKDKSQKIESGILAQRSKERPKGEVVTLEGFDKENKPVEEIKVEIQEASRP
                     QFKQVGKIDLDALVPKKKDTNEVKEEMPNIDVPLQENVEKIVTVQQVEQAPIQEVEEE
                     ITEPSVVVEAEIEDKSETENVVEDVDESVDDTISAELSDDVYTIEKNTIHSTINVVGK
                     IDLDALNQQTRPKKKTKEEKKKERLEREIQKPTPTVATAPKDAVQKEATVATDKKKRS
                     RI"
BASE COUNT         2395 a         1081 c         1274 g         2313 t
ORIGIN      
        1 aaatagatgt gttatagggt gcagagtata taactttata tagggaaata aatgaaagaa
       61 aatattgtta ctttgttgta ttttttaggc ataatgggag cgattttaat gtattttgtt
      121 tttcctaatg atattacgca tgctatttta ttcggagcta tatgtttaat tattaccatt
      181 ataccagcat tattgcttag tagaactaag aaaaagaaga agcctatata cacctataca
      241 gatgaatctt ttgctgattt tcaccctata cagtccgatt cattaagtat gattgaagag
      301 gtttctaata caactcgcaa agtctctgat agttctctta cagcaaaaag aatgtatggg
      361 aaagtgcttg cttatgtagc cgattttgaa gagctaaata aagctccact gagtgcagaa
      421 ggtcgtacgg aatgtgtgct ttttgtgtca acggtagtgt tgctcgaata taaatccttg
      481 tatcaaatag aggaataccg tgattttgaa gtggcatatt tggatattat gctttctctt
      541 gtacaccaaa atgcaacaca aaaatcgaaa gaagaagtgt atgaatttgt aaaacgtaga
      601 ttgcgttttt atacagccga atatcaaact cttatagata ataaattccc tctagagttt
      661 tattcgtact ttttttatac ccctttacgt acacgtacac aagtttgcct tgatcagaat
      721 gaattaattc gttttcatgg aaatttattg tctttggtcg aaaaaattaa gctgtcaaca
      781 aaaaatgatg caagcttttt aggcgtttag cttttctcca ataataatgc catcttgcat
      841 ctcaattatt ctgtttgaca gataagctaa gtttgtgtca tgtgttacta ctaatagggt
      901 gattcctaaa tctctgttta atgttactaa aagttgatgt aactcttctt tatttcttga
      961 atctaaactg cccgaaggtt catccgcaag aataatggag ggattgttaa ttaaagctct
     1021 tgcaacagca acacgttgtt tctctcctcc tgatagttcg ttgggtttgt gtaacaatcg
     1081 attagtaaga cctagataat gtaataattc ttgggctttt ttttcagcct cttttttttg
     1141 tacttttgca attaatgcag gtatcattac attttccaaa gccgtaaatt ctggaagtaa
     1201 ttgatgaaat tgaaacacaa aaccaatttc tttatttcgt aattcagaaa gttcatcact
     1261 atgtaggctt tccagtttca ctttattcat tgtaattgtt ccagcggttg gtttatcaag
     1321 tgttccaata atttgcagaa gtgtcgtttt tccagctcca ctggctccaa taatgctagc
     1381 aaattcgcct tttctaattg ttaaatcaat attttttagt acttgcaaag tgccaaaact
     1441 tttacaaaca ccctgtgttt caattaaatt catttttgta tcaatttttt tattacataa
     1501 gcagctaaat agcacccaaa gatagcaggt aaatatgaaa ttgtgcctac ggttgaacat
     1561 ttattttttt catcatcaat ttgtatcaca gaattttggt taacttgttc tgacgaaaat
     1621 actacaggca atccttttga gatatttacc aactttaatc tttttcttac atgttttgca
     1681 agagtacaat ggtatgtgcc tgaaatgtca gcataatgta ttttgcttgg atctgttctg
     1741 cctccagcac ccattgagga aacaatcttt attttatgtt gaatacaata acgaatgagg
     1801 tcaattttag gggatagtgt atcaatagca tcaatcacaa aatctacatg aagattttca
     1861 aacagagagg gtataagttc tttctcaata aatacatcat gttgtttgag tactaaatta
     1921 ggattgatgt caaggaggcg ttccgttaat acggttactt tttttttgcc tattgtgcta
     1981 tgtaaagcag gaagttgcct gttaatattg ctttctttta tcgtgtctgc atcaataata
     2041 gtgatgttgc ctatgcctgc acgacaaatc atttcggctg catatgcacc tacacctcca
     2101 acacctacta tcataacgtg ttttttctca aggatactac tattgttctc ccccagtaat
     2161 aatttagttc tactagtcca catactatta ttataatgaa ggtaaattca tgcctgtaat
     2221 tttacggtac aagtctaatg cgtaaatatc tgtcataccc gatataaaat ctacaatagc
     2281 ttgaattttt tcgtaagtac ttggagcgta catgtcgtat tggcttggaa ctctatccaa
     2341 taataatttt gaataggact tatttggttg aaaacttgct tttatgtatt catcaagtaa
     2401 agtttgaata attttatagc cagctacttc tatatctaat acttcttgtg atttgtatat
     2461 ttttttgaca gctattgtag agcagttttc ataagcattt ttacagactg gttcaatgtg
     2521 atctattaat gatccagaaa attgaccaga aagtatttgt tgctcatttt ccacaaatac
     2581 acgtgcacac tcagatacta actttccaat cacagaagag cgaagataag aaatttgttc
     2641 gtttgagtcg tcaactaatt tccatgtttc atagatgcgt ttttttcgtt ctgtatcgaa
     2701 aaattgcaat aataattctt ttgtttgctc tgttgataat atctttagct tatgtgcatc
     2761 ttctatatcc attatctgat aacagatatc gtcagcagct tctactatat aaactaaagg
     2821 atgtcgtgca tattttttat cagaaactgg gcttttgatt atacctagtt ccatagcaat
     2881 ttgctcatag gttgctttct ctgattcaaa aaaaccaaat ttcccttttt tgccacattc
     2941 gacagatgaa taggggtatt ttacgatcga agctaatgtg gaataagtca ttgcaaaacc
     3001 accatatcgc ctacctttaa attgatgcgt taacaatcga agagaatttg catttccttc
     3061 aaaaaaaatt aaatcattcc attcagtttc attttcaagc ctttctttta taaaagaacc
     3121 atttccttcg gaaaaaaaac tagcaatagc tttttcgcca gagtgtccaa aaggaggatt
     3181 gcccaaatca tgtgctaaac aagctgctga tactatcgat cctatttcgg gtaaaaaatt
     3241 ggagcttttc tgatgtaaaa tgcttaattg gtgagcaata ttagtgccta atgaccgacc
     3301 aacacaggca acttccaagc tatgtgttaa cctattatga acaaatacgc taccaggaag
     3361 agggaaaact tgtgttttat tttgtaaacg cctaaaagga gatgaaaaga tgagtctatc
     3421 ataatccctc tgaaattcag accgatcttc tgtgtctttt ttatgatacg cttccatccc
     3481 aaaacgtttg gtagataaaa gagtttccca ttgcattgtt gattgtttca tatgtataat
     3541 gttctatttt ctgttgaata attgcattaa aaacgcatat tgttttatct cttttcgata
     3601 agtaatataa ttaggttctt ggcttgccac taaattccaa aatgtatttc cgtgattcat
     3661 ttcttttgta tgacaaagtt catgaatgat aacatagtcg ctaagatatg ctggtacaaa
     3721 aagtaaatac gcacataaat tgatattttt tttggaggaa caactacccc aacgtgtttt
     3781 tgaacgttga atgcgaaatt cgttatacgt aaatcctccc ttatttgcta aataagctaa
     3841 tcgttgcgaa aaatgtttct ttgcttctga aactacaaat ttttccattc catcaagtaa
     3901 tatctgttgg tatttagctt gtgtaatatc caaagaaatt ggatgttcaa tcgttaaaat
     3961 tttttcgtgc aaacgaaata atatatttgt tctttcacaa gcctttgtta agatagaaaa
     4021 tgtctgtgaa tttaattccg taatatgaat agatacatgt ctcttttttt gttcggttcg
     4081 cttcaaggct ttttcaatcc aatcttgttt ggcgttgaca aaatgtagcc ctttatctat
     4141 atttacaccc aaaggaataa ttaattttat tccattatca cccacctgaa cacgtataga
     4201 acgagccttt gggctatagt aaatcaaaat tttgcctaag tctttgtgga tataagtttg
     4261 tgtcatgttt tagagcttaa gtactttcac atgcaaatgt agaaaaaaaa gctaatattt
     4321 tggcagtaaa agaattattc gtatatttgc aaccgaaaaa gagataggtg aggggggcgg
     4381 tagttcccct catttgttca cataaagtaa ggtatgatat ctaaagacgt tgttgttcag
     4441 gctgttaatc aatttttgaa agataaagaa gattatttct tagtggatgt tacaataagc
     4501 atacataaca ctatttgtgt cgaaatagat gcttttcaag gtgtagatgt agaaacatgc
     4561 tgtgaactca gtcgttttat tgaaacaaaa attgaccgag agatagaaga ttttgaatta
     4621 gaagtaggtt ctgctgggat aacagcacct tttaaaatac ttcagcaata tatcaaaaat
     4681 atcaacaacg aagttgaagt tttattgaaa aacgggaaaa aaataattgg atttttgcga
     4741 agtgccaatg agcatacatt tgtggttgaa gtgtcgcaaa aaatcaaaaa agataaaaat
     4801 gccaaaaaag aagaagtaac ggagttacta acttatgcgt attcagacgt taaacataca
     4861 aaatataatc tcagatttaa ataacaatta tgagcaacaa accagtacaa tcactgaatt
     4921 tgatagaaac attttcagag tttaaagaat taaaaaatat tgataaagcc acgatgatta
     4981 gtgtgcttga agattctttt cgtagtgtaa tagcgaaaat gtttggaacc gatgagagtt
     5041 ttacagtcgt aattaatcct gataaaggtg attgtgaaat ttggcgagac tatgaagtag
     5101 tagccgatgg tgaagtagaa gatgaggttt tacaaatggg cttgtcaaaa gctcgtgcca
     5161 ttgatgacga atgcgaaata ggtgatgatg ttacagaggc agttaacttt ttgagtttcg
     5221 gaagacgtgc tgtattaact ttgcgtcaaa cattggcttc aaaaatctta gaattacaaa
     5281 aagaatctgt atatgatcaa tacaaagaaa tggttggtca agttgtttat ggcgaagtgt
     5341 accaaatttg gaaaaaagaa atcttgttgt tagatgatca aggcaacgag ttattattac
     5401 caaaaacgga acaaatacct actgattttt ataaaaaagg agatacggta aagtctgttg
     5461 tagtgagagt tgataataaa aataatcagc tgaaaattat agtctctcgt acagctccaa
     5521 tatttttaca acgtttattt gaacaggaaa tacctgaagt gcaagaaggt actattatga
     5581 tacgccgaat cgctcgtata cctggcgaaa gagcaaaagt agccgtagaa tcatatgatg
     5641 atagaattga ccctgttggt tcttgcgtag gagtaaaagg ttctcgtatc cacagcattg
     5701 ttagggaatt gagaaatgaa aatattgatg ttattaatta tacatctaat ttatctttat
     5761 ttattgcacg ttcattaagt cctgcaaaag tgctttcgat tcgcatagac gaagaaagaa
     5821 aacgtgcaga agtgtcaatt aaaccagaag atgtgtctat ggctattggc aaaggaggat
     5881 ccaatataaa attagctaca atgcttacag aatacactat cgaagtattc agacatgtgg
     5941 atgaggaaga cgaagatgat gtgttcttag atgcattctc tgacgaaatt gaaccatggg
     6001 ttatagatgt ttttaaatct atcggatgtg atacagcaaa aagcgtatta gaaattcctc
     6061 gtcaagaatt gatcgataaa actgatttag aagaagaaac aatagatgaa gcattggcaa
     6121 tactaaggca agaatttgaa taataggtca ttttttgcaa aaggattagg gaaagatgag
     6181 cattgtcaaa aagaaaattt aattagaaga aaacaaggag tatttatatg tcgacaaaat
     6241 taaataaagt agtaaaagaa ttaaatgtag gattagctac ggctgtagag tttttacata
     6301 aaaaaggctt tgcggatgtg gaatctaatc ccaatgctcg tataaccgaa gagcaatatg
     6361 atttgctcgt caaagaattt agcaaagata aaagccaaaa aattgaatct ggtattctcg
     6421 cccaacgaag taaagaacga ccaaaagggg aagttgttac tctcgaaggt tttgataaag
     6481 aaaacaaacc agtagaagaa attaaggtgg aaattcaaga agcatctcgt cctcaattta
     6541 aacaagtggg caagattgat ttagatgctc ttgtgcctaa aaagaaagat acgaatgagg
     6601 ttaaagaaga aatgcctaat atagatgttc ctttgcaaga aaatgtcgaa aaaatagtaa
     6661 cagtccagca agtagaacaa gctcctatac aggaagtgga agaagaaata acagaacctt
     6721 ctgtggtagt agaagctgaa atagaggata aatcggaaac ggaaaatgta gtagaggatg
     6781 tagatgaatc ggttgatgat actatatctg ctgaattaag tgatgatgta tatacgatag
     6841 agaaaaatac cattcattcc acaatcaatg tggttggaaa aattgattta gatgcgttaa
     6901 atcaacaaac acgtccaaaa aagaaaacaa aagaggaaaa gaaaaaagaa aggttagaac
     6961 gcgaaattca aaaacctaca ccaacagtag ctactgcacc gaaagatgcc gttcaaaaag
     7021 aggcaacagt tgccacagat aagaaaaaaa gaagtcgtat tca
//
LOCUS       DAOPUR010000008         7060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_10637_length_7060_cov_3.036831, whole genome shotgun sequence.
ACCESSION   DAOPUR010000008 DAOPUR010000000
VERSION     DAOPUR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10637_length_7060_cov_3.036831"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PKN90_00270"
     CDS             <1..896
                     /locus_tag="PKN90_00270"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNZ84751.1"
                     /translation="ILYHPLEYNVKAETGRVDYDQMEEVAMREKPKIIVGGASAYSRD
                     WDYARMRKIADAVGAILMIDMAHPAGLIAAGLLNNPLKYAHIVTSTTHKTLRGPRGGI
                     ILLGKDFPNPWGKTTPKGEIKMMSALLDSAVFPGIQGGPLEHVIASKAVAFGEALQPE
                     YKVYQQQVQKNAAVLANAFINKGYKIISDGTDNHSMLIDLRAKFPDLTGKIAEKALEK
                     ADITVNKNMVPFDSRSPFLTSGIRVGTPAITTRGAKDDVMLEIVNLMDTVLCNPEDES
                     IITSVRKKVNDMMTAFPLFVY"
     gene            933..1871
                     /locus_tag="PKN90_00275"
     CDS             933..1871
                     /locus_tag="PKN90_00275"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNZ84752.1"
                     /translation="MNQTAPFKVFSGTKSRYLAEKICESIGCKLGEMNIQHFADGEFA
                     VSYEESIRGDEVFLVQSTFPNSDNIMELLLMIDAAKRASARKIVAVIPYFGWARQDRK
                     DKPRVSIGAKMIADMLGVAGINRLITLDLHADQIQGFFNVPVDHLYASSIFLPYIQSL
                     KLKDLVIASPDVGGSKRANTYAKHLGVDLVLCHKTRVKANEVEGLKIIGDIQGKNVIL
                     VDDMVDTAGTITKAADIMISEGALSVRAIASHAVLSDPAIERIEKSKLTEIIFTNSIP
                     LRKESPKIRILSIADLLGDTILRVNTNESISSSYLT"
     gene            1949..3358
                     /gene="rlmD"
                     /locus_tag="PKN90_00280"
     CDS             1949..3358
                     /gene="rlmD"
                     /locus_tag="PKN90_00280"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNZ84753.1"
                     /translation="MRKKKNLPLLEQITITDVAAEGKAIARYNDMVIFIPFAVPGDVV
                     DIQITRKKNSYAEGKIVTTHSYSTNRETPFCEHFGICGGCKWQNLPYASQLTYKQKQV
                     YDNLTRIGKIQSFDMLPILGSEQTQFYRNKLEFTFSNKAWMNYDMLQAGTPRTAALGF
                     HIPGMFDKVLHIEKCWLQDEISNMIRNTLHEFCQQQHISYFDLKEQHGMMRNVIVRTS
                     TTGEIMVIVVFYEKDQSIINEMLSYLLKKVPSISSLLYIHNTKANDTISDQEIHVFHG
                     SDHIFEQMEGLKFKIGPKSFYQTNSRQAYELYKITRDFASLTGNELVYDLYTGTGTIA
                     NFVANRAQKVIGIEYVPEAIEDAKVNASINNITNTSFFAGDMKDILNESFIKTQGKPD
                     VIITDPPRAGMHDDVIKAILFASPKKIVYVSCNPATQARDILLLSEKYRVIKVQAVDM
                     FPHTHHVESVALLEEKESL"
     gene            3360..3827
                     /locus_tag="PKN90_00285"
     CDS             3360..3827
                     /locus_tag="PKN90_00285"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNZ84754.1"
                     /translation="MCIQYAYYNSPIGLLEIKSTEEKLLGIQFVDTITDYSKKNCTIA
                     TLCIQQLDLYFHQQLEKFTLPIEYPSDSSNFAINVWNALLTIPYGTTYSYKELAEKIG
                     QPSSARAIGNACRKNPFVIVIPCHRVICHNGKLGGYIGGIEVKKQLVYNEKKG"
     assembly_gap    4016..4115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4559..5374)
                     /gene="ispE"
                     /locus_tag="PKN90_00290"
     CDS             complement(4559..5374)
                     /gene="ispE"
                     /locus_tag="PKN90_00290"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNZ84755.1"
                     /translation="MITYPNAKINIGLFVTEKRTDGYHNLETIFYPIPLKDKIECHIS
                     DTNDSSLIISGIRIDGNTSDNLVMKAYNLLKLSHNLPNLNFTLEKNIPFGAGLGGGSA
                     DAAFVLTLLNTTYNLGLSIAELEYIASKIGADCPFFIQNKPVFATGIGNEFHPISFSL
                     SGYFLVLVKPPIHVSTPQAYKNINPQKPDFDLKTIHLLPIKEWKHVIFNDFETNVFIQ
                     HPEIEQIKDSLYQQGALYASMSGSGSSVFGIFPKQVNITNLFQNSFVWSGFLA"
     gene            5494..6999
                     /gene="dnaB"
                     /locus_tag="PKN90_00295"
     CDS             5494..6999
                     /gene="dnaB"
                     /locus_tag="PKN90_00295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNZ84756.1"
                     /translation="MEKTKKPRKKEPEYGKVPQYVVSEYGGKLPPQAIELEEVVLGAL
                     MLEKDAYVAVCEFLKKECFYKDVHQTIYEAITNLSLNQEPVDLLTVSQQLKRMGKLEE
                     VGGQSFISELTGKVASAANIEYHAQIIVQKYLSRELIRISSDIQKKAYDEKQDVDELM
                     QDAEGQLFEITQRNLKKDVTHIQPVISKAIDLIEIAANKQDGYTGIPSGFNGIDTITS
                     GWQKSDLIIIAARPAMGKTAFVLSMAKNMALDYEHPVAVFSLEMSNIQLVNRLIVNVA
                     EIEGDKIKRGKLAPFEWEQLRLRTATLQKAPIFVDDTPSLSVFELRTKARRLVKEHKI
                     QCIIIDYLQLMNASGMTYGSREQEVSIISRSLKGLAKELDIPIIALSQLNRGVEGRTG
                     ADKRPQLSDLCESGAIEQDADMVLFIHRPEYYKITQDEQGNDLRGKAEIIIAKHRNGS
                     TGDVFLRFKQDFIKFTNLASEESYAEFQSKSTDSQEHDFLQANTYSNPSPF"
BASE COUNT         2449 a         1159 c         1207 g         2145 t
ORIGIN      
        1 gtattttgta tcatcctctt gagtataatg taaaagctga aacaggtaga gtggattacg
       61 accaaatgga agaagtagcc atgcgtgaaa aaccaaaaat tattgtagga ggagcttctg
      121 catactcacg tgattgggat tacgcacgta tgcgtaaaat tgcagatgca gttggtgcta
      181 ttctgatgat tgatatggct caccctgctg gtcttattgc tgcaggattg ttaaacaacc
      241 ctttgaaata tgcacatatc gtaacctcta ctactcataa aacattaaga ggtccaagag
      301 gtggtattat tcttttaggc aaagattttc caaatccatg gggaaaaacc acacctaaag
      361 gagaaataaa aatgatgtca gcgttattag actcagctgt ttttcctggc atacaaggag
      421 gtccactaga acatgttatt gcttctaaag ctgttgcttt tggcgaagct ttacaaccag
      481 aatataaagt atatcaacaa caagtacaaa aaaatgcagc tgttttagca aacgcattta
      541 tcaataaagg atataaaatt atatctgatg gaacggataa tcactccatg ctgattgact
      601 tacgtgcaaa atttcctgat ttaactggaa agattgcgga aaaagcatta gaaaaagcag
      661 atattactgt aaacaaaaat atggtacctt ttgatagccg tagcccattc cttacatcag
      721 gtattcgtgt tggtactcct gctattacaa ctcgtggtgc aaaagatgat gtaatgcttg
      781 aaattgtgaa tttaatggat actgtgttat gtaatccaga agatgaaagc atcataacta
      841 gtgtgcgtaa aaaagtaaac gatatgatga ctgcttttcc tttatttgtt tattaatcaa
      901 aaaaatacta tatacaagtg aaaacaaaac ccataaatca aacagctcct tttaaagtgt
      961 tttcgggcac caaatcgaga tacttagctg aaaaaatttg cgagagcata ggatgtaaat
     1021 taggcgaaat gaatattcaa cactttgctg atggtgaatt tgccgtatca tacgaggaat
     1081 ccatacgcgg agatgaagtt tttttagtac aatctacatt tcccaattcc gataatatca
     1141 tggaattatt actaatgata gatgcagcta aacgtgcttc tgcccgtaaa attgtagctg
     1201 ttatccctta ttttggatgg gctcgtcaag atagaaaaga taaacctcgc gtatctattg
     1261 gggcgaaaat gattgcagat atgctaggtg tagctggtat taatcgctta ataacacttg
     1321 atttacatgc cgatcaaata caaggatttt tcaatgtacc agtcgaccac ttatatgcct
     1381 catcgatttt tttaccttat atccaatcgc ttaaattaaa agatttagtg attgcatctc
     1441 ctgatgttgg tggatcgaaa agagccaata catatgctaa gcatttaggt gttgatttgg
     1501 ttctttgtca taaaacacgt gtaaaagcta acgaagtaga agggttaaag attattggag
     1561 acattcaagg taaaaatgta atattagtag atgatatggt tgatactgct ggcacaatta
     1621 ccaaagctgc agatataatg atatccgaag gagctctctc agtgcgtgct attgcttcgc
     1681 atgctgtatt gtctgatcct gccatagaac gtatagaaaa atcgaaacta accgaaatta
     1741 tttttacaaa ttccattcct ctaagaaagg aaagtccaaa aatccgaatt ttgagtattg
     1801 cagacttatt aggcgataca attcttcgtg taaacaccaa tgaatcaatt agttccagtt
     1861 atttaacata acagaagaca tctatttaca aacatagaga tagctaccct cgggtagtta
     1921 tttctattta atcactacac ccaatactat gcgaaaaaag aaaaatcttc ctttactaga
     1981 gcaaataact attacagatg tagcagccga gggaaaagcc atagcacgct acaatgacat
     2041 ggtaattttt attccatttg ctgtacctgg cgatgtggta gatatacaaa ttacacgcaa
     2101 aaaaaattcg tatgctgaag gaaaaatagt aactacgcat tcttattcaa cgaaccgtga
     2161 aacaccattt tgcgaacatt ttggaatatg tggtggatgt aagtggcaaa atctacccta
     2221 tgcttcccaa ctaacataca aacaaaaaca ggtgtatgat aatttaacac gcattggaaa
     2281 aattcaatct tttgacatgt tacccatttt ggggtctgaa caaactcaat tttatcgcaa
     2341 caaacttgag tttacgttct caaataaagc atggatgaat tatgacatgc tacaagctgg
     2401 tacgcccaga acagcagcat tgggttttca tattccaggt atgtttgaca aagtattaca
     2461 tattgaaaaa tgttggctac aagatgaaat atccaatatg attagaaata ctctacatga
     2521 attttgtcaa caacaacata ttagctattt cgatttaaaa gaacaacatg gcatgatgcg
     2581 taacgttatt gtacgcacta gcactacggg cgagattatg gttattgtag ttttttatga
     2641 aaaagatcaa tcaatcatca atgaaatgct aagctattta ctcaaaaaag tgccatctat
     2701 cagttcattg ttatacattc ataatacaaa agcgaatgat actatttctg atcaagaaat
     2761 acatgtattt cacggttctg accatatatt tgaacaaatg gaagggctaa aatttaaaat
     2821 cggacctaaa tcattttatc aaacaaatag tcgtcaagcc tacgagttgt ataaaattac
     2881 acgtgatttt gcttcactta caggcaacga actggtgtat gatttgtata ctggcactgg
     2941 tactattgct aactttgtgg caaatagagc tcaaaaagta attggtatcg aatatgtacc
     3001 cgaagccata gaagatgcaa aagtaaatgc ctctatcaac aatattacca atacatcttt
     3061 ttttgctggc gacatgaaag atatcttgaa tgaatcattc attaaaacac aaggtaaacc
     3121 agatgtgatt ataacagatc ctccacgtgc aggtatgcat gatgatgtga ttaaggcaat
     3181 tttgtttgca tctcccaaaa agattgtata tgtaagttgc aatccagcaa ctcaagcccg
     3241 agatattcta ttactatccg aaaaatatcg tgttataaaa gtacaagccg tggatatgtt
     3301 tccacatacg catcacgtag aaagtgttgc attgctcgaa gaaaaagaaa gtttataaaa
     3361 tgtgtatcca atatgcctat tacaactctc ctatcggttt gttagaaata aaatcaaccg
     3421 aagaaaaact tttaggcata caatttgttg atacaattac agattacagc aaaaaaaatt
     3481 gtactattgc tactctttgt atccaacagc ttgatctcta ttttcatcaa cagttggaaa
     3541 aatttacttt gccaattgaa tatccatcag atagctcaaa ttttgcgata aacgtttgga
     3601 atgctctttt aactataccg tatggtacta cttatagcta caaagagtta gctgaaaaaa
     3661 ttggtcaacc aagcagtgct agggcaatag gaaatgcctg tagaaaaaat ccttttgtaa
     3721 tagtaatacc ttgtcataga gttatttgcc acaatggtaa attaggaggg tatataggag
     3781 gcatagaagt aaaaaaacag ttagtgtata acgaaaaaaa aggctaagta tatactactc
     3841 agccttcttc tccttttatt tattaactac tcattatcag gggttgtata accaaagata
     3901 aaatcaaaaa aattaaatat gtttcccatt cctttatata atattccttc ttctttcata
     3961 attgtaagtt ttaactagtt taacacttat cgccaatctt ctacttattt acgatnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctct atatgttatt gtgttataca
     4141 tgactgtaaa atttaaaatt tatatagttt tccgacattt agaaacacta tttacttttt
     4201 ttcttttcgc ttatttttct ttcagaactt gaatgtccca agataatatc aaataatttt
     4261 aaatctccaa gtcctttata tgccatcgct ttgtacatag ttgtaaattt aattggttca
     4321 acactttttt gtaacgaatc ttgcggttag caatcatttc cattttattg acccttaatt
     4381 ttctaaaact aaagagtcaa aaatctgatt ctgtgtcata attttgtctc ctttcattat
     4441 tttaattatt catttgttag ccattcgctc tcgaatgcat tacaaatata tgctgtaaaa
     4501 catatatttt acaactttcg tcaaataata tgataaaaat gagaagaaaa ataggctttt
     4561 aggctaaaaa accagaccaa acgaaggaat tttgaaacaa atttgtaata tttacctgtt
     4621 ttgggaagat tccaaagaca gaagaacctg accctgacat ggatgcatat aaagctccct
     4681 gctggtacaa actatcttta atctgttcaa tttctgggtg ttgaataaaa acattcgttt
     4741 caaaatcatt aaaaataaca tgtttccatt cttttattgg tagtaaatga attgttttta
     4801 aatcaaaatc aggtttttga ggattgatat ttttatatgc ctgcggagtt gatacgtgaa
     4861 taggcggttt tactaaaaca aggaaataac cagacaaaga aaatgaaatg ggatgaaact
     4921 cattaccaat ccctgtagcg aatactggtt tattctgtat aaaaaaaggg caatctgcac
     4981 caatcttgga tgcaatgtat tctaattcgg caatggataa acctagatta taggtcgtat
     5041 ttaataaggt aagaacaaaa gcagcatctg ccgaaccacc tcccaatcct gcaccaaaag
     5101 ggatattctt ttctaaagta aaattaagat ttggtaagtt gtgcgataat ttaagcaaat
     5161 tgtatgcttt cattactaaa ttatctgagg tattgccatc tatacggata cctgatatga
     5221 taagtgaaga atcgtttgta tccgaaatat gacactctat tttatctttt aatggaatag
     5281 gataaaatat cgtttctaaa ttgtggtaac catcggttcg tttttcggta acaaacaacc
     5341 caatatttat ttttgcattc ggatacgtaa tcatatatat agaataatag tgcaaaggta
     5401 taacttttca acaattcaga atgaggattg ttcaaaaata ccttttctaa atatgaaaaa
     5461 aagtgctatt tttgaacctc attagcaaag accatggaaa agacaaaaaa accacgcaaa
     5521 aaagaaccag aatacggaaa agtacctcag tacgttgtat ctgaatacgg aggaaaactg
     5581 ccacctcaag caattgaatt ggaagaagtg gttttagggg ctttgatgtt agaaaaagat
     5641 gcttatgtag cggtgtgcga atttctcaaa aaagaatgtt tttacaagga tgtacaccaa
     5701 acaatatacg aagcaattac caatctatcg ttaaatcaag aacctgttga cctactaact
     5761 gtatcccaac aattaaaacg tatgggaaaa ttagaagaag taggaggaca atcttttatt
     5821 tcagaactaa cagggaaagt ggcttctgcc gctaatatag aatatcatgc tcaaattatt
     5881 gttcaaaagt atttgtcgag agaattaata cgtatctcgt ctgatatcca aaagaaggcg
     5941 tatgatgaaa agcaagacgt tgatgaatta atgcaagatg ctgaaggaca gctttttgaa
     6001 attacacaac gaaaccttaa aaaagacgtt acacacattc aaccagtcat aagcaaagct
     6061 attgatttga ttgaaattgc tgccaacaaa caagatggat atacaggcat tccatcaggc
     6121 tttaatggca ttgatacgat tacatctgga tggcaaaaat ccgaccttat tattatagct
     6181 gcacgtccag ctatgggaaa aacggctttt gtactgtcta tggcaaaaaa tatggcttta
     6241 gattatgaac atccagtagc tgttttctct cttgaaatgt ctaatataca attagttaac
     6301 cgtttaattg taaatgtagc tgaaatagag ggagacaaaa ttaaacgtgg aaagttagct
     6361 ccatttgagt gggaacaatt gcgtttgcga actgcaacat tgcaaaaagc cccaattttt
     6421 gttgatgata cccctagcct ttcagtattt gagttacgaa caaaagcccg ccgattagtt
     6481 aaagagcata aaatacaatg tattataatc gactacttgc aattaatgaa tgccagcggc
     6541 atgacgtatg gaagccgtga acaagaggta agtattattt cgaggtcatt aaaaggcttg
     6601 gcaaaagaac ttgatattcc aatcattgct ttatcacagt taaatcgtgg tgtagaagga
     6661 cgtactggag ctgacaaacg ccctcaatta tcagacctgt gcgaatcggg agcaatagaa
     6721 caagatgcgg atatggtact gtttatccat cgccctgaat attataagat tacacaagac
     6781 gaacaaggaa acgatttaag aggtaaagcc gaaattatta ttgccaagca tcgaaatggt
     6841 tctacaggcg atgtattttt acgtttcaaa caagacttta ttaaatttac caatcttgca
     6901 tcagaagaat cgtatgctga atttcaaagt aaaagtacag atagtcaaga acatgatttt
     6961 ttgcaagcaa atacgtattc taatccttca ccattttaaa ctatatttat attcatgcaa
     7021 aatttatatc atctaggtat tgacgacatc aatatgaata
//
LOCUS       DAOPUR010000009         7043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_10663_length_7043_cov_3.834574, whole genome shotgun sequence.
ACCESSION   DAOPUR010000009 DAOPUR010000000
VERSION     DAOPUR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10663_length_7043_cov_3.834574"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PKN90_00300"
     CDS             <1..523
                     /locus_tag="PKN90_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ84757.1"
                     /translation="IDASRGVESQDLNIFSIIQKNRKSLVVCVNKWDLVENKETNTSK
                     EFEQTIRDRFAPFVDFPIIFASALTKQRIFKVLETATEVYENKTQHISTSVLNDFFLP
                     LIENYPPPALKGKYVKIKYVTQIREAKTPTFIFFANLPQYVKDPYKRFLENKIRANWN
                     FHGNPIQVFIRKK"
     gene            654..1832
                     /locus_tag="PKN90_00305"
     CDS             654..1832
                     /locus_tag="PKN90_00305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ84758.1"
                     /translation="MRRFLTVFAAFIIMLCLGSVYAWSVIASELMSVYHFSAAQSQII
                     FGTIIAIFPITMIFVGQLARKLHPRYLGYISGVLFLSGYQLAAHANGHFFLTYLGLGI
                     TAGIATGFGYWVSLTIPVQWFPEKKGLITGVAAAGFGLGAVFISAIAEKMLIAGKDVL
                     EVVQFIGIVYGLTVILFSNLITPNSDLSKSGVVAIRIKECISSTIFLKLFSGIFLGTF
                     AGLLIIGSLKIIGEQSNINNHILILGVSVFAATNFLGRLIWGFLSDYIGASLSIFLAL
                     LIQSLAILSLNLFSLSDHTYLILSFLIGFGFGGNFVLFAKEAAQLYGVQKLGLIYPYV
                     LLGYAIAGIAGPAVGGILFDFSNSFFYAILVASIMSLIGSVLFLKQYINARRNTLAKN
                     "
     gene            complement(1866..2204)
                     /locus_tag="PKN90_00310"
     CDS             complement(1866..2204)
                     /locus_tag="PKN90_00310"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HNZ84759.1"
                     /translation="MKAVDYFHKKPALLNCAQAILTAWQKEYDISENTILEFKQYGGG
                     RAPEGACGALYAAEYLMKKHHQQSIYKEFSVYAGSIACKEIRKNGVVSCQKCVEIADE
                     LLQNARVKKE"
     gene            complement(2280..4409)
                     /gene="pnp"
                     /locus_tag="PKN90_00315"
     CDS             complement(2280..4409)
                     /gene="pnp"
                     /locus_tag="PKN90_00315"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNZ84760.1"
                     /translation="MMNVITKTITLGDGRTISIETGKLAKQADGSVMVRMGNTMLLAT
                     VCAAANAVPGTDFMPLSVDYKEKFAANGRFPGGFTRREGRASDYEILVSRLVDRALRP
                     LFPDDFHAETFVNVTLFSADGEDMPDALAGLAASAALAVSDIPFNGPISEVRVARIDG
                     QFVVNPTFSALKNADMDIMVAATMDNIMMVEGEMKEVSEDDLLQAMKVAHEAIKVHCQ
                     VQLDLMEAAGKTQKRVYNHEDNDEELKKDIWAKTYDAVYAVASACNPDKHSRIANFEA
                     VKETYIASLTPEVAAEKKGLISKYYHDVEKEAMRRAILDEGKRLDGRKTTEIRSIWSE
                     IDYLPGAHGSAIFTRGETQSLTTVTLGTKQDEKLIDEALTQGKERFLLHYNFPPFSTG
                     EARASRGTGRREIGHGNLALRALKGMIPDNYPYVVRVVSEILESNGSSSMATVCAGSL
                     ALMDAGVQIKKPVSGIAMGLISENKGKNFAVLSDILGDEDHLGDMDFKVTGTRDGITA
                     TQMDIKVDGLSYEILEKALSQAKEGRMHILNKIEETIATPRPDFKANAPRIVTLQVPK
                     DMIGAIIGPGGKIIQAIQLESGASVSIEEKENVGVVEIAANNKQAIDIALAKVKGIVA
                     VPEVGEVYKAKVKSIMPYGAFVEFMPGKEGLLHISEISWERIEDMQLTGIKENDEIEV
                     KLLEVDKKTGKFKLSHKVLTQRPPRKE"
     gene            5103..>7043
                     /locus_tag="PKN90_00320"
     CDS             5103..>7043
                     /locus_tag="PKN90_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNZ84761.1"
                     /translation="MKEFFTTKFRTWGEWIKQFIKQLVEWRKYRYHRFKNLVWYKKLA
                     NATATSFGLFLLLLLLIDINFLWLFGSSPSLYSISHPDQNIASEIISADKKVIGKYFT
                     ENRIPVTYNEISPILIKTLIATEDERFYEHFGIDFQGVMAAIKDMTKGNARGASTITQ
                     QLVKNMYKTRNQYSKGLFGYIPFVKLLIMKAKEWITAVKIEFFYSKEEILTMYFNTVD
                     FGSNAFGIHTAAKTYFKTTPDKLNYEQSATLVGLLKATTTYNPRLNPKKSTERRNVVL
                     QNLVSQGILTQIETDSLKAIPMKLDYSVEQSYDGKALHFRAFLSKYLDPWEKQTGYDI
                     YADGLKIYVTIDSRLQQYAEESVQKQMRTVQRRFNSHWGNENPWQDENRVEIPDFVEK
                     IARKTTTYSNLHKKYKGNKDSIDVYLNLKKKTKVFDYTYGEKDTLFSVMDSIRYMSKF
                     MHCSFMAMEPTSGEVKAWVGDINFPFWQYDKVGQSKRQPGSTFKLFVYTAAMMNGYTP
                     CDKLTDQQTIWNYVENGEPKTWIPRNAEVRYTGYNMTLKNAFARSINTIAVQLAQKIG
                     IPEVIKYAHLMGIKTPLENMPSTSLGSSDVSLLELVNAYATVINEGKYHEPILVTRIE
                     DRHGRIIYQANRTQKQVIPYETA"
BASE COUNT         2268 a         1313 c         1150 g         2312 t
ORIGIN      
        1 gattgatgct tctagaggcg tagaaagtca ggacttaaat atattctcca tcatacaaaa
       61 aaaccgcaaa agcttggttg tatgtgtcaa caaatgggat ttagttgaaa acaaggaaac
      121 gaatacgagc aaagaatttg aacaaaccat tcgggataga tttgcccctt ttgtggattt
      181 ccctattatt tttgcatcag cccttaccaa acaacggatt tttaaagtgc ttgaaacagc
      241 taccgaagta tatgagaata aaacacagca tatttcaacc agtgtgctca atgatttttt
      301 cttacctcta atcgaaaatt atccaccgcc agctcttaaa ggtaaatatg tgaaaattaa
      361 atatgtaaca caaatacgtg aagcaaaaac acctacgttt atcttttttg caaatttacc
      421 gcaatacgta aaagaccctt acaaacgttt tttagaaaat aaaatcagag ctaattggaa
      481 ttttcatgga aacccaattc aagtatttat cagaaagaag taagccatac ggcttgcttc
      541 tttctttata tgtgaatacg tcagcataaa atgtatattg tttagccaaa ctataatcaa
      601 tccttaccag atataatatc ttcagttaat acactattgt aatccataaa tttatgagaa
      661 gatttttaac cgtatttgcc gcatttatca tcatgctttg tttagggagt gtatatgcat
      721 ggagtgtaat tgcatccgaa ttaatgtctg tgtatcattt ctcggcagct caatcacaaa
      781 ttatcttcgg caccataatt gccatatttc ctatcactat gatttttgtt gggcaattgg
      841 ctcgtaaact acacccacgg tatttgggat atatttctgg agttcttttt ttgagcggat
      901 accaattagc cgctcatgcc aatggtcatt ttttccttac ttatcttggg ctagggatca
      961 ccgcaggaat tgcaacaggt tttggatact gggtttctct aaccatccct gttcaatggt
     1021 ttcctgaaaa aaagggctta attacaggcg tagccgcagc tggttttgga ttgggggctg
     1081 tatttatctc tgctattgcc gaaaaaatgt tgatagcagg gaaagatgtg ctagaggtag
     1141 tgcaatttat tggtattgta tacggcttaa ccgttattct attttcgaat ctaattactc
     1201 caaatagtga tttatctaag tcgggagtgg ttgctatccg aataaaagaa tgtatatcat
     1261 caacgatttt cctcaaacta tttagcggta tttttttagg aacatttgct ggtctgctta
     1321 ttattgggag tttaaaaatt attggtgaac aaagcaacat caacaaccac atactaatac
     1381 ttggtgtatc cgtatttgca gcaaccaact tcttggggcg tcttatttgg ggattcttaa
     1441 gtgattatat cggggcaagt cttagtattt ttttagcatt gctcatacaa tcattggcta
     1501 ttttatccct caatcttttt tctttatcag accacaccta tctgatactg tcatttttaa
     1561 taggttttgg atttggaggt aattttgtac tatttgccaa agaagcagct caactatatg
     1621 gtgttcaaaa acttgggttg atataccctt atgttttgct aggatatgcc attgctggta
     1681 tagctggacc tgcggttggt ggcattctgt ttgatttttc aaattcattt ttttatgcta
     1741 ttttagtagc aagtatcatg agcttaatag gcagtgtgct ctttttaaaa caatacatta
     1801 acgcgagaag aaatacatta gcaaaaaact agtgtaatcg ttgctacaaa aattgagagt
     1861 gctgtttatt cttttttgac acgggcattt tgcaacaatt catctgcaat ttctacacat
     1921 ttttggcaac tcactactcc attttttcta atttctttgc atgcaataga gcctgcatat
     1981 actgaaaact ccttgtaaat tgattgttga tgatgttttt tcattaaata ctcagcagca
     2041 tacaatgccc cacaagcacc ctcaggagca cgtccgcctc catattgttt aaattctagg
     2101 attgtattct ctgaaatgtc atactctttt tgccacgcag ttaatatggc ttgtgcacaa
     2161 ttaagcaatg ctggcttttt atgaaaataa tcaacggctt tcatttcttt tatcttttat
     2221 ttaaaaaaag caccacattc tgaaaagaag tggtgctttg attttgattg aacaatatac
     2281 tattcttttc gaggaggtct ttgtgtcaat actttatgcg ataatttaaa tttacctgtt
     2341 tttttatcta cttcaagtaa tttaacctca atctcatcat tttcttttat tccagttaac
     2401 tgcatatctt cgatacgctc ccaactaatc tcagaaatat gaagcaaacc ttctttgcct
     2461 ggcataaatt caacaaatgc accataaggc ataatagatt ttacctttgc tttatacact
     2521 tcacctacct ctggaactgc aactataccc tttactttag ctaaagcgat atcaattgct
     2581 tgtttattat tagctgcaat ttctacaaca ccaacatttt ccttttcttc gatagatact
     2641 gaagctccag attctaattg aattgcttga attatttttc caccaggtcc tataattgca
     2701 ccaatcatat ctttaggtac ttgtaatgtt acaatacgag gagcatttgc cttaaagtca
     2761 ggacgtggag tagcaatagt ttcttcaatt ttatttaaga tgtgcattct gccttctttg
     2821 gcttgcgaaa gagctttctc taaaatttcg tacgacaaac catccacttt aatgtccatt
     2881 tgtgttgcag taataccatc gcgtgtacca gttaccttaa agtccatatc acccaaatga
     2941 tcttcatctc ctaaaatatc agaaagaaca gcaaagtttt ttcctttatt ttcagaaatc
     3001 aaacccatag caataccaga tactggtttt ttaatttgca cacctgcatc catcaatgcc
     3061 aacgaacctg cacacactgt agccatagac gaagaaccgt ttgattctaa gatttctgat
     3121 actacacgta ctacatatgg ataattatct ggaatcatgc cttttaatgc tcttaaagct
     3181 agatttccat gaccaatttc acgtcttcct gtaccacgag atgctcttgc ttcaccagta
     3241 gagaatggag gaaagttata atgcaataga aaacgttctt tgccttgagt taaggcttcg
     3301 tcaattaatt tttcatcttg ttttgtccca agagttactg ttgtaagtga ttgagtttcg
     3361 ccacgagtaa aaattgctga accatgagct cctggtaaat agtctatttc tgaccaaata
     3421 gaacgaattt ctgttgtttt tcttccgtcc aaacgcttac cctcatcaag aatagcacga
     3481 cgcatagctt ctttttctac atcatggtaa tatttgctta tcaaaccttt cttttcagcg
     3541 gctacttctg gagttaaact agctatatac gtttccttta ctgcttcaaa attagcaata
     3601 cgagaatgtt tatctggatt acatgcacta gctactgcat acactgcatc ataggttttt
     3661 gcccaaatat cttttttcaa ttcttcatca ttatcttcgt ggttatacac acgcttttgt
     3721 gtttttccag cagcttccat caagtcaagt tgtacttgac aatgtacttt aattgcttcg
     3781 tgagcaactt tcattgcttg caataaatca tcttcagata cttctttcat ttcaccttct
     3841 accatcatga tattatccat agtagcggca accataatat ccatatcggc atttttcaat
     3901 gcagaaaatg taggatttac tacaaactga ccatcaatac gagctacacg tacttcagaa
     3961 atgggtccat taaaaggaat atcagatact gctaaggcag ccgaagcggc taaaccagca
     4021 agtgcatcag gcatatcttc tccatcagca gaaaaaaggg ttacattaac aaacgtttct
     4081 gcatgaaaat catctggaaa taatggacgt aaagccctgt caaccaatcg ggaaacaagt
     4141 atttcataat cagatgcacg accttcacga cgtgtaaaac cgccagggaa acgaccgttt
     4201 gcagcaaact tttctttata atcaacagaa aggggcataa aatcagttcc aggaactgca
     4261 tttgcggcag cacaaactgt tgccaataac atggtattac ccatgcgtac catcactgaa
     4321 ccatcggctt gctttgccaa ttttccggtt tcaatgctaa ttgttcttcc atcaccgagt
     4381 gtgatagttt ttgtaattac attcatcata ctaaaaatta catgttgttt atatattctt
     4441 aaaatttcga cacaaagata cgttttaatc cttgaaaatg agtttatttt taaggatttg
     4501 tgttgcatat gggtcaaaaa aagttgtttt taatcaaaca gaatattttt caacatttac
     4561 tcattcatgt gttattggtc tctttgaatt atatcttcag acacattggt tactatattt
     4621 tacaacatac aagcatagtt ttgtgtaaaa aatttggata ataaacaaaa agagtgtatc
     4681 tttgaagtac cgtatcgcat ttggtgaaac ttaacactct tttacatact gagactattt
     4741 tcaatttcta atgacgtgct gctattggaa acaagtaggc agattacaaa ggaaattgag
     4801 cactcaaaac tttagatatc gaagtttgac tcgtaaatag cggtacggtt tttttttgtt
     4861 agcgtaatgc taactttttt atttctacaa aacagaaaac actaactatc aaacaattac
     4921 cacaaccccc ctcaattctt ctgaatactt gttttactta tttaagaaca agataaatat
     4981 atcggcaata aaattttgac agaaacattc ttttaaataa ctttgacgcc acaatatgaa
     5041 gtaagttttt cctacttaac acctatattc atattgaaac caatagtgct tttttagata
     5101 ctatgaaaga attttttaca acaaaatttc gtacttgggg agaatggatt aaacaattta
     5161 ttaaacaatt ggttgaatgg cgtaagtatc gttatcatcg ttttaaaaat ctagtttggt
     5221 ataaaaaatt agccaatgca acagcaacat ctttcggtct ttttctatta ttactcttat
     5281 taattgatat caattttttg tggttgtttg ggtcttctcc aagtttgtat agcataagcc
     5341 acccagacca aaacattgct tcagaaatta tttctgctga caaaaaagta atcggcaaat
     5401 attttaccga aaatcgcatt cccgttacct ataatgaaat ttctcctatt cttataaaaa
     5461 cgcttattgc tacggaagat gaacgctttt atgagcattt tggcattgat tttcagggag
     5521 taatggctgc tataaaagat atgacaaaag gaaacgcacg aggagctagt accattacac
     5581 aacaactagt aaaaaacatg tataaaacgc gtaatcaata ttcaaaagga ttattcggtt
     5641 atattccatt tgttaaatta ctgataatga aagccaaaga atggatcact gcggttaaaa
     5701 tagaattttt ttattcgaaa gaagagatac taacaatgta ttttaatacg gttgattttg
     5761 gaagtaatgc ttttggaatc catacggctg caaaaacgta ttttaaaacg actccagaca
     5821 aactcaatta cgaacaatca gctactttag ttggattatt aaaagcaact acaacctata
     5881 atcctcgatt aaatcctaaa aaatcaactg aaaggagaaa tgtggtatta caaaatcttg
     5941 tttcgcaagg tatccttaca caaatagaaa cagattcatt aaaggctatt cctatgaaac
     6001 ttgattatag cgtagaacaa tcatacgatg ggaaagcctt gcattttaga gcttttttat
     6061 ctaaatattt agacccgtgg gaaaaacaaa cgggctatga catctatgca gatggcttaa
     6121 aaatatatgt taccattgat tcgcgtctgc aacaatatgc agaagaatcc gttcaaaagc
     6181 aaatgcgaac agtgcaacgg cgttttaata gccattgggg caacgaaaac ccttggcaag
     6241 atgaaaatag agtagaaata ccagactttg tagaaaagat tgcacgtaaa acaactacat
     6301 acagcaatct gcataaaaag tataagggca ataaagactc catcgatgta tatttaaacc
     6361 taaagaagaa aacaaaagta ttcgactata cgtatggaga aaaagacaca ttatttagtg
     6421 ttatggattc aatacgctat atgagtaaat ttatgcattg tagttttatg gctatggagc
     6481 ccactagtgg tgaagttaaa gcatgggttg gagacatcaa cttcccattt tggcaatacg
     6541 ataaagtagg acaatcaaaa cgacaaccag gatcaacgtt taaattattt gtttataccg
     6601 ctgctatgat gaatggctac actccttgtg acaaattaac cgatcagcaa accatttgga
     6661 attatgtaga aaatggagaa cccaaaacat ggattcctcg caatgcagaa gttaggtata
     6721 ctggatacaa catgactttg aaaaatgcgt ttgctcgttc aatcaatacc atagccgtac
     6781 aactagctca gaaaattggc attccagaag tgattaaata cgcacatcta atgggaataa
     6841 aaaccccttt agaaaatatg ccatctacct cattaggttc atcagatgta tctctacttg
     6901 aacttgtaaa tgcatatgcc acagttatta atgaagggaa ataccacgaa cccattttag
     6961 taacacgcat agaagaccgc catggaagaa taatttacca agctaatcgt acacaaaaac
     7021 aggttatccc ctacgaaaca gct
//
LOCUS       DAOPUR010000010         7016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_10713_length_7016_cov_3.349662, whole genome shotgun sequence.
ACCESSION   DAOPUR010000010 DAOPUR010000000
VERSION     DAOPUR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10713_length_7016_cov_3.349662"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..2773)
                     /locus_tag="PKN90_00325"
     CDS             complement(80..2773)
                     /locus_tag="PKN90_00325"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNZ84762.1"
                     /translation="MKIHFKLNYFTKWGQNIAIMGSIPELSNNDPSKALYMNFEWKED
                     WSLDIDVQRDAPFELTYKYVLKDTNGLDVLEWGDDRTILVDPLRDENIYCYDSWNPAG
                     AVENVFMTSPFQNVLFKENHIPVPAITPKKYTHIFRVKAPTLKKGEALCVVGSTKELG
                     DWASEKPVVMSNEDKNWWVAKVNLATTKVDVVNYKYGVYDIEDQSLKYFEYGADRKAT
                     VVTTKKSLVIVSDSFARIGSYDFKGAGVGIPVFSIRTKSSFGVGDFIDIKLMVDWAKK
                     VGLKLIQVLPLNDTIGTHTAADVLPYAAISAFALSPLYLNLPKMGKLPADNPLNEAYK
                     TKQKALNDLPLVEFLEIVNFKLAYSKALYLENKEKFLKNKSYLKFFKENKHWLVSYAA
                     FCYLRDKNGTADHSKWGEYATFSEAKLERLTSPEQAHFDDIAVNYFIQYHLHLQLLEA
                     AEYAHENGVILKGDIPIGVNRNSVDTWVAPELYNMHMQAGAPPDMFAIKGQNWELPTY
                     NWEKMEETGFDWWKKRFQQMGYYFDTFRIDHILGFFRIWQIPLHQEEGIMGYLNPSIP
                     VHINEFGEKGVHFDYNRFCVPFITDAVLWEVFGDDANWVKTNCIDIIDGWILRLKPIC
                     NTQKKVMEMFDKNMITEKIKWGLFDLISNVLFFEVEWSNGMMYYPRYGMQTTTSFRYL
                     DNFTKNKIEELYVDYFYRRQDSFWKYSALKKLPAIKRSTNMMICGEDLGMMADCVTSV
                     MNELGILSLEIQRAPKVDTIEFFHPADAKYLSVVTPSTHDMSTIRGWWEEDREVTQRF
                     YNQQLGHWGEAPYFAEWWVCRDMIVQHLYSPAMWAIFQLQDLLSISDTLRRQNPHEER
                     INVPSNSKHSWRYRMHLTVEELIEQETFNKELKNYIVQANR"
     gene            complement(2870..5203)
                     /locus_tag="PKN90_00330"
     CDS             complement(2870..5203)
                     /locus_tag="PKN90_00330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNZ84763.1"
                     /translation="MKPKTKKTILIGLWTLFASIIVGTVLLFFLIAKGVIGYIPEIEE
                     LQNPKNKFASEIYTADGEVLGRFFYGKDNRVAVNYTQISPYMIEALVATEDIRFFNHS
                     GIDARSLGRAIILRGLLGKASAGGGSTITQQLAKLLYSPNTDNLFQRAMQKPIEWVIA
                     VQLERFYAKEEIIAMYLNQFDFLNNAVGIKSASQVYFNVAPNELNREQAALLVGMCKN
                     PSLFNPNRFPERAKNRRNTVLDQLYKYDYITKQEVDSLKSIPIKLNFQRVDHKLGLAP
                     YFREYLRQTMMADKPQRSNYMSWQMQKYKEDSLAWETNPLYGWCKKNKKPNGSNYNIY
                     ADGLRIYTTIDARMQRYAEEAVKEHIGDNLQKRFFNEKRGRSYAPFSRNITPEEMSSI
                     LTKAKRQSDRYRNMKKANYTNAEIDRAFAKKIPMSVFTWTGVRDTIMSPMDSIKWQKH
                     FLRCGFMSMDPYNGHVKAYVGGPNFAEFQYDMVTQGKRQVGSTIKPYLYTLAMEEGLT
                     PCQKVPNIPQTFYLEDGKEWTPRNASNSKEGELVTLRWGLANSNNWITAYLMKQITPQ
                     ALVRMIHSFGIQSHMEPVVSLCLGPADVSVKEMVGAYSSFVNKGIRVEPLFVTRIEDN
                     KGNVLATFTPQLVEIFSEETSYKMLTLLGAVVNEGTGVRLRYRFQLRGEIAAKTGTTQ
                     NNSDGWFIATTPQLVAGVWVGGEDRSIHFDNIADGQGANTALPIWAIFMKKVYADTSL
                     PYSENVKFDIPTDIRQGYDCRSEPNTEESFDQIFDLE"
     gene            5481..>7016
                     /locus_tag="PKN90_00335"
     CDS             5481..>7016
                     /locus_tag="PKN90_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ84764.1"
                     /translation="MNTFENLGLEQSILQGISEMGFVSPTPIQEEVIPYMLSNQSDII
                     GLAQTGTGKTAAFGLPLIQLISTKERTTQGLVLAPTRELCMQIATELRKYSTYKEGIH
                     IVAVYGGEDIMKQLKQLDKTPHIVVATPGRLIDLINRRKVSLQGIKHLILDEADEMLK
                     MGFLDDITTIIEETPDSRQTLLFSANMPTEISSIARRYMSNAKEIAVGKKNEASKNVE
                     HVCVVVRKDQKYLALKGILDSNPDIYGIIFCRTRQQTKDIADLLIVDGYNADALHGDL
                     SQAQRDMVMHKFRIKNLQILVATDVAARGLDVNNLTHVINYDLPDDKETYTHRSGRTG
                     RVNKSGISIAIVTPREKGSLSTIEKAINRKFKQELIPTGLEACQKQLNHLIHRMREVE
                     VSEDLDDNYLSEIFEMLTDMSKEEVITRFVSLEFNRFLSYYKDSKDLNNDIKAEKDGG
                     EARNRRTGKSDRIRLKIDQGKNDGYEPKRLLKLINEVTDSKDINIGDIEVTSKFTFFD
                     VDKD"
BASE COUNT         2243 a         1291 c         1141 g         2341 t
ORIGIN      
        1 gcgtttaacg tttagtgttt atttctgtca actacttaaa aaaaatgtcc aagaaattct
       61 tggacatttt ttttaataat taacgattag cttgtactat gtagtttttc agctctttat
      121 taaaggtttc ttgttcgatt aattcttcta cggtaaggtg cattctgtat ctccaactat
      181 gtttggagtt tgatggcaca tttattcgct cttcatgtgg attttgacgt cttaatgtat
      241 ctgaaatact taataaatct tgtaactgga atattgccca catagctgga gaatataagt
      301 gctgaacaat catatctcta catacccacc attcggcaaa gtaaggagct tcaccccaat
      361 gaccaagttg ttggttgtaa aatcgttggg taacttccct atcctcttcc caccagcctc
      421 tgatggtgct catatcgtgg gtagaaggtg taactactga taaatattta gcatcggcag
      481 gatggaagaa ttcgatggtg tcaactttag gagcacgttg tatttctaaa cttaaaatac
      541 ctaattcatt cataacgcta gttacacaat ctgccatcat acctaagtct tcgccacaaa
      601 tcatcatatt ggtagaacgt ttgatagctg gtagtttttt taatgctgaa tatttccaaa
      661 aactatcttg acgacgatag aaataatcta catacagttc ttcaatttta tttttcgtaa
      721 aattatctaa ataacggaaa ctagtagtgg tttgcatacc ataacgtggg taatacatca
      781 ttccattcga ccattcaact tcaaaaaaca atacatttga aattaaatca aataagcccc
      841 acttaatttt ttcagtaatc atgtttttat caaacatttc cattactttc ttttgggtat
      901 tgcatattgg tttaaggcgt aaaatccaac catcaatgat atcgatgcaa tttgttttca
      961 cccagttggc atcatcacca aatacttccc acaatactgc atcggtaata aatggcacac
     1021 agaatctatt gtaatcaaaa tgtacgcctt tttcgccaaa ctcattaata tgtactggaa
     1081 ttgatgggtt taaatacccc ataatacctt cttcttggtg caaaggaatt tgccagatac
     1141 ggaaaaatcc taagatatgg tcgatacgga aggtgtcaaa ataatacccc atttgttgaa
     1201 aacgtttttt ccaccaatca aatcctgttt cttccatttt ttcccaatta taggtaggaa
     1261 gttcccagtt ttgacctttg atagcaaaca tatcgggtgg agctccagct tgcatatgca
     1321 tattatataa ttcaggagca acccatgtat caacactgtt tctgtttaca ccaataggaa
     1381 tatctccttt cagaattact ccgttttcat gagcatattc agctgcttct aataactgta
     1441 aatgcaaatg gtattgtata aaataattta ctgcaatatc atcaaaatga gcttgttctg
     1501 gagatgttaa tctttccaat tttgcctcag aaaatgttgc atattcgccc cattttgagt
     1561 ggtctgcggt accgttttta tcacgtaaat agcaaaaagc agcgtaagaa accaaccaat
     1621 gtttgttttc tttgaagaat tttaagtacg atttattttt cagaaatttc tctttatttt
     1681 ctaggtataa tgcttttgaa taggctaatt taaaattcac aatttctaga aactctacca
     1741 aaggcaaatc atttaatgct ttttgtttgg ttttgtatgc ttcatttaac ggattatctg
     1801 ctggcaactt acccatttta ggtaggttta aatacaaagg acttaaggca aaagcagaga
     1861 tagcagcata aggcaataca tcggctgcgg tatgtgtacc aatggtgtca tttaaaggta
     1921 aaacctgaat aagtttcaga cctacttttt ttgcccagtc aaccattaat ttgatgtcga
     1981 taaaatcacc taccccaaaa cttgattttg tgcggatact aaacacagga atacctacac
     2041 cagcaccttt aaaatcgtat gatccaatac gtgcaaaaga atcagaaacg ataacgagcg
     2101 atttctttgt tgttactacg gtagcttttc tatctgcacc gtattcaaaa tatttaagag
     2161 actggtcttc aatatcatat actccatatt tataattcac aacatcgacc ttggttgttg
     2221 ctaaatttac ttttgctacc caccaatttt tgtcttcatt gctcatcaca actggtttct
     2281 cacttgccca atcacctaat tctttagtgc ttcctaccac acacaacgct tccccttttt
     2341 tcaaggttgg agcttttact ctaaaaatat gtgtatattt tttaggggta atggcaggta
     2401 cagggatatg attttctttg aataatacat tttgaaaggg tgaggtcata aacacatttt
     2461 ctacagcacc agcaggattc caactatcat agcaatatat attttcatct cttaatggat
     2521 cgactaaaat tgttcggtca tcaccccact ccaacacatc caatccattg gtgtctttta
     2581 atacgtattt gtatgttaat tcaaatggag catcacgttg cacgtcaata tccaacgacc
     2641 aatcctcttt ccattcaaaa ttcatataca atgccttaga aggatcattg ttcgataatt
     2701 ctgggataga ccccattatg gcaatattct gcccccattt tgtaaagtaa tttaatttga
     2761 aatgaatttt catgtttctt ttctattttt agggttaatc ttacgtttta aatgtatttt
     2821 tcaaaggtac aacaaatttt catatcgaca aatagtctgg tttatttcgt tattctaaat
     2881 cgaatatttg atcaaaactt tcttctgtat ttggttcaga acggcagtca taaccttgtc
     2941 gaatatctgt aggtatatca aatttaacat tttctgaata aggcaaacta gtatctgcat
     3001 acactttttt cataaaaatt gcccaaatag gtaaggctgt attagcccct tgaccatctg
     3061 ctatattgtc aaagtgaatg gatctgtctt caccaccaac ccatactccg gcgactaatt
     3121 gaggagttgt agcaataaac caaccgtctg aattattttg cgttgtacct gttttagctg
     3181 ctatttcccc tctaagctga aaacgatagc gtaggcgaac accagtaccc tcatttacaa
     3241 cggctcctaa gagtgtcagc atcttatagg aagtttcttc gctaaatatt tctaccaatt
     3301 ggggagtaaa agttgctaac acatttcctt tattatcttc tatacgtgta acaaaaagcg
     3361 gttcaacacg tatacctttg tttacaaaag aagaataggc tcccaccatc tctttaacag
     3421 acacatcagc aggtcctaaa cacaatgata caaccggctc catatgactt tgaataccaa
     3481 aagaatgtat catcctaacc aatgcttgtg gggtaatttg tttcattaaa taagctgtaa
     3541 tccagttgtt tgaatttgcc aatccccaac gtaacgtaac taattctccc tctttcgagt
     3601 tgcttgcatt acgtggtgtc cattcttttc catcctctag ataaaaggtt tgcggaatat
     3661 tgggcacttt ctgacaagga gtcaaccctt cttccatagc cagtgtatat aaataaggct
     3721 tgatggtaga acccacttgt cgttttcctt gcgttaccat atcatattgg aattcagcaa
     3781 agtttggacc acctacatag gcttttacat gtccgttata gggatccata gacataaatc
     3841 cacagcgtaa aaagtgtttt tgccatttga tagaatccat tgggctcata atagtatcac
     3901 gaacacctgt ccaagtaaat acactcatcg gtattttttt agcaaaagca cgatcaattt
     3961 cagcatttgt ataattggct tttttcatat tgcgatacct atctgactga cgttttgctt
     4021 ttgttaaaat gctagacatt tcttctggag taatatttcg agaaaatgga gcatacgaac
     4081 gtccacgttt ttcgttaaaa aaacgttttt gcaaattatc tccaatatgc tctttcactg
     4141 cctcttctgc ataccgttgc atgcgagcat ctatcgtagt ataaatgcgt aatccatctg
     4201 cataaatatt gtaatttgac ccatttggtt ttttattttt tttacaccaa ccatacaatg
     4261 gatttgtttc ccaggctaac gagtcttctt tatatttttg catttgccac gacatataat
     4321 tgcttctttg gggtttatca gccatcatcg tttgacgcaa atattctcta aaataaggag
     4381 ctaaacctaa cttatgatca actcgttgga aattcaattt aattggaatt gattttaatg
     4441 aatctacttc ttgtttagta atataatcat acttatacaa ttggtcaagt actgtattac
     4501 gcctattttt agcacgttct ggaaagcggt ttggattaaa taacgaaggg tttttacaca
     4561 ttcctacaag caaagctgct tgttctctat ttagttcatt gggtgcaaca ttgaaatata
     4621 cctgtgaagc tgattttata ccaacggcat tattcaaaaa atcaaattgg ttaagataca
     4681 ttgcaattat ttcctcttta gcataaaaac gttctagttg tacagctatc acccattcta
     4741 taggtttttg catagcacgc tgaaagagat tatctgtatt aggagaataa agtaatttag
     4801 ctaactgttg tgtaatagta ctacccccac ccgcactggc ttttccaagt aaacctcgta
     4861 aaataatagc tcttcccaaa gaacgtgcat caataccaga atggttaaaa aaacgaatat
     4921 cttctgtagc taccaatgct tctatcatgt aaggagatat ttgggtataa tttaccgcta
     4981 ctcggttgtc tttaccatag aaaaatcttc ctaaaacctc tccatctgcc gtgtatattt
     5041 cagatgcaaa tttatttttt ggattttgca actcttcaat ttctggtata taaccaataa
     5101 caccttttgc tataaggaaa aatagcaata ctgtaccaac tatgattgac gcaaaaagtg
     5161 tccaaagtcc aataagaatt gtttttttag tttttggttt catgtagtag tttcttgata
     5221 aatttttgcc cacaaaggta cagtattttt ttcaaacgac tagtaaagac tctattttta
     5281 aacaaaaaag tatgaatatc attattattc aaaaataaaa aactaccttt gtaccgcaat
     5341 aattatttat tttatataca acatctttta tatagtgaaa aaataggttt actaaatgac
     5401 acggactgat tttgccgttt ttaatacgca tccctctttg ttttttcgct atttattttc
     5461 ctcatcattt attattttac atgaacacat ttgaaaattt aggtcttgaa caatcaattc
     5521 ttcagggcat tagtgagatg ggatttgttt ctccaacacc catacaagaa gaagttatcc
     5581 cttacatgct ttctaatcaa tctgatatca ttggtttagc acaaacagga acaggaaaaa
     5641 cagcagcttt tggtttgcct ctaattcagt taatcagcac aaaagaaaga acaacacaag
     5701 gtttagtctt agctccaaca cgtgaattat gcatgcaaat agccacagaa ttacgtaaat
     5761 atagtaccta caaagaaggt atacatatcg tagcggtata tggtggtgaa gacattatga
     5821 aacaattaaa acaactcgat aaaacaccgc atattgtagt agctactcca ggtagattaa
     5881 ttgatttgat taatcgtaga aaagtttcgt tgcaaggaat taaacattta atattagacg
     5941 aagctgatga gatgctaaaa atgggatttt tagatgatat aacaaccatc atcgaagaaa
     6001 ctcctgatag ccgacaaaca ttattatttt cggctaatat gcccacagag ataagcagta
     6061 ttgctcgtcg ttatatgagc aatgcaaaag aaattgctgt tggtaaaaaa aatgaggcat
     6121 ccaaaaatgt tgagcatgta tgtgtggtag tacgtaaaga ccaaaaatat ttagctctaa
     6181 aaggaatttt ggatagcaac ccagatattt atggcattat attttgtaga actcgccaac
     6241 aaaccaaaga tattgcagat ctacttatag tcgatgggta taatgctgat gccttgcatg
     6301 gcgatttgtc gcaagcacaa cgtgacatgg taatgcataa attccgtatt aaaaatttac
     6361 agattttagt agctacagat gttgctgcac ggggattaga tgtaaacaat ttgacacatg
     6421 ttattaacta tgatttgcct gatgataaag aaacgtacac ccatagaagc ggacgtacag
     6481 ggcgtgtcaa taaatcagga atttctattg ctattgtaac tcctagagaa aagggttctc
     6541 ttagcacaat tgaaaaagca ataaatagaa aatttaaaca agaactcatt cctacaggat
     6601 tagaggcatg ccaaaaacaa ttaaaccacc ttatacaccg tatgcgtgaa gtggaggtta
     6661 gcgaggattt agatgataac tacttgagcg agatttttga gatgctaaca gatatgagca
     6721 aagaagaagt gattactcgt tttgtttcgt tagaatttaa tcgcttttta agttattaca
     6781 aagattctaa agatttgaat aacgatatta aggcagaaaa agatggaggc gaggcacgca
     6841 atcgtagaac aggaaaatcg gatagaattc ggttaaaaat agaccaaggt aaaaacgatg
     6901 ggtatgaacc aaaacgatta ttaaaactca ttaatgaggt aactgatagt aaagatatta
     6961 atattggaga tattgaagtt acatctaaat ttacattttt tgatgtagat aaagac
//
LOCUS       DAOPUR010000011         6952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_10844_length_6952_cov_3.192982, whole genome shotgun sequence.
ACCESSION   DAOPUR010000011 DAOPUR010000000
VERSION     DAOPUR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10844_length_6952_cov_3.192982"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="PKN90_00340"
     CDS             <1..1924
                     /locus_tag="PKN90_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNZ84765.1"
                     /translation="LAYFSCDFGIMVLNLAKKEIANTYIIGDNATMSAVYAVNSDETY
                     LYALTSESIKKAPKQGVNLLNFENWLVAMSLPDDSQLFRDMDVFNNKFYVAKNNENVY
                     VYEEGQWLVFYANDAQLAISIQTIGNHFIINVSNSLLCYTKDLTVEQLTDIVVSDSWY
                     DDTEGVYWIASKTSALLRYKKGEGVLGRYKPSSPYLSTAQKMYYNNGRILVAPGKSWD
                     DRYSIPGAVLLFEDEKWTEYTSENSGVLQYSYWFADVVSIAVDPVDKNKLYVSTWGEG
                     VYVFENGVATQLFNGLNTEGVLETAVPSKHNFYRIDGLVFAKNKNLWMVNSLASKGLK
                     MKTYDGKWHSFNYPSLANKFLLRHLLLHSRGQKWIVSSTSSGRGIFVIDDNGTILDES
                     DDRTRFYTSFTDRDGNVIVPSYFYSIAEDKDGTVWLGTDKGPLLMTNVSKVFDNTYTC
                     TRVKIPRNDGTILADYLLDNEEIRAIAIDAANRKWIGTGNSGVYLLSANGRETIHHFT
                     VDNSPLSSNKIESIVIQEETGEVFFSTQDGLISYRSDATVGKKSFEKVEVFPNPIRPD
                     FQGLITVRGLVANTEVRIVDQSGLLVFSGVATGGSIVWDGMNFANHPVGSGVYYVFSV
                     NADGTEHGSTKFMIIR"
     gene            complement(1921..2394)
                     /gene="folK"
                     /locus_tag="PKN90_00345"
     CDS             complement(1921..2394)
                     /gene="folK"
                     /locus_tag="PKN90_00345"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNZ84766.1"
                     /translation="MNVVYLALGSNIGNKKRILSEAESLINEKIGTVIHASSVHTTNP
                     EGFISTHKFLNKVICVYSILSPQKILETILEIEKKLGRNAKSIDGNYQDRTIDIDILY
                     YNDSIINVETLTVPHPHLHTRVFVLKPLLEIAPELIHPILQLSTKQMLLRLEKED"
     gene            complement(2400..4199)
                     /gene="typA"
                     /locus_tag="PKN90_00350"
     CDS             complement(2400..4199)
                     /gene="typA"
                     /locus_tag="PKN90_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNZ84767.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKMLLAGSLFKEHEKVGELMMDN
                     NDLERERGITIVSKNVSIRYKDYKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKAIQIGLKPIVVINKVDKPNCRPDEVHEAVFELMFSLDASEEQLDFP
                     TIYGSAKQNWMSDSWSKQTDTIFPLLDAIIEHIPAPKKLEGTPQLLITSLDYSSYVGR
                     IAIGRIHRGTLKENMDVALVKRDGSIVKSRIKELNVFEGFNKLKVPSVSSGEICALIG
                     IDNFDIGDSITDLEKPDPLPPIAIDEPTMSMVFTINDSPFFGKDGKFVTSRHIHERLM
                     KELDRNLALQVVKSETADSWNVYGRGVLHLSVLIETMRREGYELQVGQPQVIFKTIDN
                     VKHEPIELLTINVPEEDSGKVIELVAVRKGEMTIMERKGDRVHMEFIIPSRGIIGLRT
                     NMLTATAGEAVMAHRFIEYAPYKGEIERRSNGSIIAMESGTAFAYAIDKLQDRGKFFI
                     FPQDEIYAGQVVGENSKEGDMVVNVTKSKKLTNMRASGSDDKVRIVPPIVFSLEEALE
                     YIKEDEYVEITPNSMRLRKILLDENDRKRAKAK"
     gene            complement(4264..5097)
                     /gene="murI"
                     /locus_tag="PKN90_00355"
     CDS             complement(4264..5097)
                     /gene="murI"
                     /locus_tag="PKN90_00355"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNZ84768.1"
                     /translation="MHKGSRISRIGVFDSGYGGLTILEEFRKKLPEYDYVYLGDNART
                     PYGTRSFEVVYQYTLECVKKLAKMDCELIILACNTASAKALRTIQQKDLPILFPQKRI
                     LGVIRPTVEQLDTCTQSKHVGILGTEGTIQSQSYTIEINKLYPHITVVDQACPMWVPL
                     IENNEHESDGADFFVHKYLSNLLNKDKFIDAIVLGCTHYPLLLKKIKHILPSHINIIS
                     QGEIVAQSLENYLLRHPEIETKLAKNHQCMFYTTESELKFISSASIFLKETIKAERIS
                     L"
     gene            complement(5166..5684)
                     /locus_tag="PKN90_00360"
     CDS             complement(5166..5684)
                     /locus_tag="PKN90_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNZ84769.1"
                     /translation="MKKSIIISLCIAFSFSLFAQTNDVPKFGHINKQDVFSLMPEADA
                     AMKELEALLLNYKVESKKLEEEFTKKQQDYVANQATLDAAIKKYRETELQRLYASIEE
                     FSKNADEALKKRQSELLAPIEAKINDAIKTIGDENGFIYIFDVTTPGTFAYMSTKSVD
                     ILPLVKQKLNLK"
     gene            complement(5719..6234)
                     /locus_tag="PKN90_00365"
     CDS             complement(5719..6234)
                     /locus_tag="PKN90_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNZ84770.1"
                     /translation="MKKTLLLVGIFVLSFTAMQAQRFALVDIEYILQQIPTYESATEQ
                     LNQSSKQWEKEIEALTTEAQTLYKNYQTEMVFLSDEMKVKREEAIIAKEKEAMDLKKK
                     YFGAEGELFKKKESLLKPIQDEIYNAVKEISENKNIQLILDRASGNNIIFASPTIDIS
                     DEVLQRMGYVR"
     gene            complement(6256..>6952)
                     /locus_tag="PKN90_00370"
     CDS             complement(6256..>6952)
                     /locus_tag="PKN90_00370"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ84771.1"
                     /translation="GDFEIQGKRILSLVQSYTHLQPHHHVLDVGCGIGRFAIPLTSFL
                     STNGTYEGFDIVKTGIDWCKKHITPSYPHFNFSHIDLNNTLYNTATKAKAEQFKFPYS
                     KSYFDIVVLTSVFTHMMPNDVANYIEQISDVLKPEGMCYATFFLFNKQENFSSTERSF
                     QFSYDYGHYRLLDKKVKEANIAFDEAFIHSLAEKHQLSIKHSIKGWWRGIEKTDKIDF
                     QDIIVFEKRNQLS"
BASE COUNT         2068 a         1128 c         1233 g         2523 t
ORIGIN      
        1 tttagcgtac ttttcatgtg attttggaat tatggtatta aacttagcta aaaaggaaat
       61 tgccaatacc tatatcattg gagataatgc cactatgtct gcagtatatg ctgtaaattc
      121 cgatgaaacg tatttatatg cacttaccag tgaatctatt aaaaaagcac ctaaacaagg
      181 tgtaaattta ttgaattttg aaaattggtt ggttgctatg agtttgcctg atgattcgca
      241 gttatttaga gatatggatg tatttaataa taagttttat gtcgccaaaa ataatgagaa
      301 tgtatatgtg tatgaagaag gtcaatggct agtgttttac gctaatgatg cacagctagc
      361 tatttctatt caaacgatag gtaaccattt tattataaat gtttccaatt ccttgttgtg
      421 ttatacgaaa gatttaaccg tagaacaact gactgacata gtagtatctg attcgtggta
      481 cgatgataca gaaggcgttt attggattgc atcaaagaca tcagcacttc ttcggtataa
      541 aaaaggggaa ggggtattag gtagatataa gccgtcatct ccgtatttaa gtactgctca
      601 aaagatgtat tataacaatg gtagaatatt ggttgctcct ggcaaaagtt gggatgatag
      661 atatagtata cctggagcag ttttattatt tgaggatgaa aaatggactg aatatacatc
      721 cgaaaattcg ggagtgttac agtattctta ttggtttgca gatgtggttt ctatagcagt
      781 tgatcctgtg gataagaata agttatatgt tagtacatgg ggagaaggtg tgtatgtgtt
      841 tgagaatggc gtagctactc aattgtttaa tggtttaaat acagaaggtg tgttagaaac
      901 ggcagtgcca agtaaacata atttttatag aatagatgga ttagtttttg ctaaaaataa
      961 aaatttatgg atggttaatt ctcttgctag taaaggttta aaaatgaaaa cttatgatgg
     1021 aaaatggcat tctttcaatt atcctagttt agcaaataaa tttctgttaa ggcatttatt
     1081 attgcattct cggggtcaaa agtggattgt ttcgtctaca agttcaggga gaggaatttt
     1141 tgtgatagat gataatggaa ccattttaga tgagtcggat gatagaactc gtttttatac
     1201 ttcttttact gatagagatg gaaatgttat tgtcccttct tatttttatt cgatagctga
     1261 agataaagat ggaacagtgt ggcttggaac agataaaggt ccattgttga tgaccaatgt
     1321 gagcaaagta tttgacaaca catatacatg tactagggtt aaaatcccta ggaatgatgg
     1381 tacaatattg gctgattatt tattagataa tgaagaaata cgtgctatag caattgatgc
     1441 agctaataga aaatggatag gcacaggcaa ttcaggagta tatctattgt cagcaaatgg
     1501 tcgggaaaca attcatcatt ttactgtaga taatagtcca ttgtcttcga ataaaattga
     1561 atccatcgtt attcaagaag aaactgggga agtgtttttt tctacacagg atggtctaat
     1621 ttcttatcgt tcagatgcta cagtaggaaa aaaaagtttt gaaaaagtag aagtatttcc
     1681 taatcctata cgacccgatt ttcaggggct aattactgtt agagggttag ttgccaacac
     1741 agaagttcgt attgttgatc agtcaggttt gttggttttt tctggtgtag ctactggggg
     1801 ttcaattgtg tgggatggga tgaattttgc caatcatcct gttggctctg gtgtatatta
     1861 tgtattttct gttaatgccg atggcacaga gcatggctca actaagttta tgattattcg
     1921 ttagtcttcc ttttcaagtc ttaaaagcat ctgttttgta gatagttgaa gaattggatg
     1981 tatcaattct ggggctattt ctaaaagggg ttttaataca aacactcgtg tatgcaaatg
     2041 tggatgagga actgttaggg tttcaacgtt aataatagaa tcattataat ataaaatgtc
     2101 aatatcaatt gttctatctt ggtagtttcc atctatagat ttggcattcc ttccaagttt
     2161 tttttcgatt tcgagaatgg tctctaagat tttttgtgga cttaaaatag aatacacaca
     2221 aattacttta ttaagaaatt tgtgtgtaga aataaatcct tcagggtttg ttgtatggac
     2281 tgaagacgca tgtataactg taccaatttt ctcgttaata agggattcag cttccgatag
     2341 aattcgcttt ttattcccaa tattacttcc tagagctagg tatacgacat tcattgattt
     2401 tattttgctt tcgctctttt tctgtcattt tcgtcaagaa gaatttttcg caatcgcatt
     2461 gaatttggtg ttatttcaac atattcatct tctttaatgt attctaaagc ctcttctaaa
     2521 ctaaatacaa taggaggaac gatgcgtact ttgtcatccg aaccagatgc acgcatattc
     2581 gttagtttct tcgatttcgt tacatttacc accatatctc cttctttaga gttttcacca
     2641 actacttgtc cagcgtatat ttcgtcttga ggaaaaataa agaactttcc tctatcttgt
     2701 aatttgtcaa tagcatacgc aaatgcagta ccactttcca ttgcaataat tgatccattg
     2761 cttcttctct caatttcacc tttgtaagga gcatattcta taaaacgatg agccattaca
     2821 gcttcacctg ctgtagccgt tagcatattg gttcgtaagc caataatccc cctagaggga
     2881 attataaatt ccatgtgtac ccgatcacct ttacgttcca taatagtcat ttctccttta
     2941 cgcacggcaa ctaactcaat tactttacca gaatcttctt ctggtacatt tatggttaat
     3001 aattcaatag gttcatgttt tacattatca attgtcttaa aaataacctg tggctgtcct
     3061 acttgtaatt catatccttc gcgtcgcatt gtttcgatta atacggataa gtggagtact
     3121 ccacgtccat atacgttcca cgaatcggct gtttctgatt ttacaacttg taatgctaag
     3181 tttctgtcta attctttcat caaacgctcg tgaatatgcc gtgaagtaac aaattttcca
     3241 tccttaccaa aaaatggaga gtcgttaatt gtaaatacca tactcattgt aggttcgtcg
     3301 atagcgatag gaggtagcgg atctggtttt tcaagatcag ttatgctatc gccaatatca
     3361 aaattatcaa taccaattaa ggcacaaatt tcacctgagg atacacttgg tacttttaat
     3421 ttgttaaacc cttcaaatac atttaattct tttattcgag attttacgat acttccatcg
     3481 cgtttaacta aagccacatc catgttttct tttaatgtgc ctctatgaat gcgtccaatt
     3541 gctatacgac ctacatacga tgaataatct aaggatgtta ttaataattg aggggtacct
     3601 tccagttttt taggagcagg aatatgttca atgatagcat ctagtaaagg gaaaatagta
     3661 tctgtttgtt tggaccaaga gtcagacatc cagttctgtt ttgcagagcc ataaatagtt
     3721 gggaagtcta gttgttcttc tgaagcatcc aaactaaaca tgagttcgaa tactgcttcg
     3781 tgcacttcat ctgggcgaca atttggttta tctactttgt ttattacaac gattggcttt
     3841 agacctattt gaatggcttt ttgcaacacg aaacgtgttt gtggcattgg accttcaaat
     3901 gcatccacta atagcaacac accatcagcc atatttaaca cacgttctac ttcaccacca
     3961 aaatcggagt gaccaggagt gtcaatgata ttaattttgt aatctttata gcggatagaa
     4021 acatttttag atacaattgt aatgcctcgt tctcgctcaa ggtcattatt atccatcatt
     4081 aattctccca ctttttcgtg ttctttgaac agtgaacccg ctaataacat tttatcaact
     4141 aatgttgttt ttccgtggtc aacgtgtgca ataatcgcaa tatttctaat cttttgcata
     4201 tagtttttat ttgaggatgc aaaggtagtc agaatatttt tatctgtact ataattttga
     4261 ttattacagc gaaattcttt ctgcttttat cgtttctttt aagaagatgg aggcggagga
     4321 aataaattta agctcagatt cagtagtata aaacatacat tggtggtttt tagctaattt
     4381 tgtctctatt tcaggatgac gcaaaagata gttctctaaa ctttgagcaa caatttcgcc
     4441 ttgagagata atattgatat gtgacggtaa gatgtgctta atttttttca ataacaatgg
     4501 gtaatgtgta catcccaata caatcgcatc aataaatttg tctttgttaa gtaagtttga
     4561 aagatactta tgcacaaaaa aatcagcacc atcactttca tgttcattgt tttcaataag
     4621 aggcacccac atcggacaag cttgatccac tactgtaata tgcggatata gtttatttat
     4681 ctctatagta tacgattgag attgaatggt gccttccgtt cccaaaatgc caacatgttt
     4741 agattgggtg caagtgtcta gttgttctac tgttgggcga attaccccta gtattcgttt
     4801 ttgcggaaat agtattggca agtctttttg ttgaatcgta cgtagtgctt ttgccgaagc
     4861 tgtattgcaa gccaaaataa ttaactcaca gtccatcttg gctagtttct ttacgcattc
     4921 cagtgtgtac tgatacacta cttcgaacga acgtgttcca tatggcgttc gagcattatc
     4981 tccaaggtac acataatcat actcgggtag tttttttcta aactcctcta agatagttaa
     5041 tcctccataa ccagagtcaa aaacacctat acgggatatt ctacttcctt tatgcatata
     5101 aaacaacagg aagatagagt acactatttg tctacttttc ttcctgtttg ctatcagtaa
     5161 attgtttatt ttaaattcaa cttttgtttt actaatggta aaatatctac acttttggtc
     5221 gacatataag cgaatgtacc aggtgtggtt acatcaaaaa tatagataaa tccgttttcg
     5281 tctccaatcg ttttgatagc atcatttatt tttgcctcaa tcggtgctaa taattctgat
     5341 tgtcttttct ttagggcttc gtctgcattt tttgaaaatt cttcaatact tgcatataaa
     5401 cgttgtaatt ctgtttctct atattttttt atagcagcat caagtgttgc ttgatttgca
     5461 acataatctt gttgtttctt tgtaaattct tcctctagct ttttactttc tactttgtag
     5521 ttaagcaata aagcttccag ctctttcatt gctgcatcgg cttctggcat taaagaaaag
     5581 acatcttgtt tattgatatg accaaatttt ggtacatcat ttgtttgtgc aaataaagaa
     5641 aaagaaaatg cgatacataa gctaataatg atgctttttt tcataatgtt ttttttattt
     5701 taatgagtaa aattggtttt atcgtacata tcccatgcgt tgtaatactt cgtcgctaat
     5761 atcaatagta ggggatgcaa aaataatatt atttccagac gctctgtcca aaattaattg
     5821 aatattttta ttttctgaga tttcttttac agcattataa atctcatctt gaataggttt
     5881 gagaaggctt tcttttttct taaatagttc tccttcagca ccaaaatatt tctttttcaa
     5941 atccattgct tctttttctt ttgcaatgat tgcttcttct ctttttacct tcatttcatc
     6001 tgataaaaat accatttctg tttgataatt tttatacaat gtttgagctt cagtggtgag
     6061 agcttcgatt tctttttccc attgttttga actttggtta agttgttctg tagcagactc
     6121 gtaagttggt atttgttgca atatatattc gatatctacc aatgcaaatc gttgagcctg
     6181 catcgctgtg aaactaagga caaaaatgcc tactaataga agtgtttttt tcatgtcttt
     6241 tacattttaa gattactaac taagttgatt tcttttttca aaaacaataa tatcttgaaa
     6301 atcaatttta tctgtttttt ctatacctcg ccaccaacct ttaatactgt gtttaatact
     6361 aagttgatgt ttctcagcta aagagtgaat aaatgcttca tcaaatgcaa tattagcttc
     6421 ttttactttt ttatcaagca agcgataatg tccgtagtcg taactaaatt gaaagctcct
     6481 ttctgttgag gagaaatttt cttgtttatt aaataggaag aatgtggcat aacacatccc
     6541 ttctggtttt aatacatcac taatctgttc tatataatta gcaacatcgt ttggcatcat
     6601 atgtgtgaat accgatgtta gaactacgat atcgaagtac gactttgaat aaggaaattt
     6661 aaattgctca gcttttgctt tggtggcagt gttgtataag gtattgttta agtcaatatg
     6721 tgaaaaattg aaatgtggat atgacggagt aatatgtttt ttgcaccaat ctatacctgt
     6781 tttaacaata tcaaaaccct cataagtgcc attggttgat aaaaaagatg tgagtggtat
     6841 agcaaatcgc ccaattccac atcctacatc caatacatga tggtggggtt gaaggtgtgt
     6901 atatgattga accaaagaaa gaatacgttt accttgtatt tcaaaatcgc cc
//
LOCUS       DAOPUR010000012         6814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_11136_length_6814_cov_3.545791, whole genome shotgun sequence.
ACCESSION   DAOPUR010000012 DAOPUR010000000
VERSION     DAOPUR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11136_length_6814_cov_3.545791"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PKN90_00375"
     CDS             complement(<1..1327)
                     /locus_tag="PKN90_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ84772.1"
                     /translation="METNTELELAWRCVVQTGTLLFLTGKAGTGKTTFLHQLKTTSPK
                     RMVVLAPTGIAAINAGGVTIHSFFQLPFAPYIPGTEFATDTKYQFNKEKINIIRSLDL
                     LVIDEVSMVRADLLDSVDAILRRYRRTTVPFGGVQLLLIGDLHQLSPVIKEEERRLLE
                     AYYPSSYFFNSKALQKTAYLTIELKQVYRQSEKHFLDLLNQIRDNKATSTTLEELNKR
                     YIPDFIPPAGEEYIRLTTHNHQAQTINDQHLAKISSPAFSYKAQVNGNFPQHAYPADE
                     LLTIKEGAQIMFIKNDVSFPKRFYNGKIGKVTHVDEHVIEVQTNDDPEPFSLQKAVWT
                     NAKYVLNKETNEISEDIEGTFEQYPIRLAWAITIHKSQGLTFDKAIIDIASSFAHGQT
                     YVALSRCRTLNGMVLNSPISHRAIIKDAVIHDFVQQSATDAPDESKIAVL"
     gene            1485..1955
                     /locus_tag="PKN90_00380"
     CDS             1485..1955
                     /locus_tag="PKN90_00380"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNZ84773.1"
                     /translation="MESTSFMQLAIDLAIENVKQGKGGPFGAVVVKEGKIVGQAANSV
                     TSTNDPTAHAEVNAIRQACAHLKTFDLSGCELYASCEPCPMCLAAIYWARIDKVYVSA
                     TKYDAAEVGFDDAFIYEEFALPKENRKVLQVPLLVQEGKAPFRAWKQKIDKIPY"
     gene            complement(2045..2929)
                     /locus_tag="PKN90_00385"
     CDS             complement(2045..2929)
                     /locus_tag="PKN90_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNZ84774.1"
                     /translation="MKNKNYASDIKTVIDELTGLCPTENMFHPNKEGEPFPSRAIMKD
                     SIKLTRSILFPGFFGNASVNKTTLPYHIGVNVEKLFTMLWEQVYAGLCFDCKEEDIDL
                     EAKREKAFTITLSFVKQLPRIKEVLRTDVEAMYKMDPAAKSYGEIIISYPGLRAITNY
                     RIAHELHKLQTPIIPRIITEMAHAETGIDIHPGARIGESFAIDHGTGVVIGETCIIGN
                     NVRIYQGVTLGAKSFPVDENNIPIKGIDRHPIIKDNVIIYASATILGRIIIGENAVIG
                     GNTWITEDVQPNEKVFIK"
     gene            complement(2948..4522)
                     /locus_tag="PKN90_00390"
     CDS             complement(2948..4522)
                     /locus_tag="PKN90_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ84775.1"
                     /translation="MKTPILSILLLVGWFATFAQTSAQFEMQKLNFSYGAITNFYVDK
                     VDPSKLTEWAIKGMLDELDPHSSYLSADEVKESTEALQGSFDGIGVSFNMVEDTLVVI
                     QSITGGPSEKLGILPGDKIILVNDTTIAGVKMKTSDITKRLKGPKGTQVKVTILRNNE
                     SIPFTIIRDRIPIFSVDAFYMVDNKIGYIKVNRFASTTHQEVVDGIKNLKKKGMESLI
                     IDLQGNGGGYLQSAIEIVDELLSNKRTVVYTEGANHAKQTMTTRRNGLMESGKVVVLV
                     DEYSASASEIVAGALQDWDRGVVIGRRTFGKGLVQRPIPMPDGSEIRLTVARYYTPSG
                     RSIQRPYEKGKEKYEEDILDRYKHGEFVSADSITFADSLTYKTLVTQRTVYGGGGIMP
                     DIFVPADTTKYTTLHRQLIRNHIITKISIVYAEKNRALVSKQFSTFEAFKLKFDTQEV
                     LSQIKQETLAKEIAFTEEEWNETKDLLAIQAKAIIARNLWSTNEYFEIINETNDFVKK
                     AIEVLKSESIYYNTLQ"
     gene            complement(4628..6289)
                     /locus_tag="PKN90_00395"
     CDS             complement(4628..6289)
                     /locus_tag="PKN90_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNZ84776.1"
                     /translation="MNKPTTFLFFRPLIALFILLHVVLVSILAQTVDLKITTFNTEWL
                     SCDTNGPSDEEGQMNNIVSLIQTINPDIIALQEVGTSITYATIDTLVKKLGINEWAGD
                     IVATTPTNCGQNQGIIYKKSHLQLVNSSLLSNGANSQGNSYYYNWTNGRYPVIHDFVL
                     TIGSTTIPFTIINIHAKAMSDETSYSRRLGASEGLKGILDGTNYNTSKIVITGDFNDY
                     LIGSQTATYSNSPYKNFMDDTENYQSLTSSLTDPYYSSPVIDNIIITNELASHYVANS
                     TIRETSATNTISNYRNTTSDHTPISALFRFTSNNPTDCQAIDYSESFASSLGDFIPYS
                     VEGTQTWHWRNIYGAYVSGYASSTNNPNEDWLISPAFDLSDKESGSLSFEHALNYAQE
                     ASERASNHTLWISTNYTEGDPKNANWTQLSIPTMPLGNSWTYVNSGNINLSSQWMKEN
                     VRFAFKYIATSSMAGTWEIKNLLFNATCEPVAIPSISNDNKVVFAQNRNISVLLEHET
                     SIRIFNIVGNTLYNDTRSENSVFPVSQAGIYLVCIGNECYKIAVR"
     gene            complement(6453..>6814)
                     /locus_tag="PKN90_00400"
     CDS             complement(6453..>6814)
                     /locus_tag="PKN90_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ84777.1"
                     /translation="IQASYAYVLLNKDAGELISKYALDYLQHKINLQYVHGISKQVDA
                     SWVVSYQDRKGNYVNKDGNKVAYTPFATIDTRINWQQNNTKMYAEISNILNQHYYDYG
                     GVPIAGRMLKIGIAVQL"
BASE COUNT         1867 a         1265 c         1373 g         2309 t
ORIGIN      
        1 ctaacaccgc tattttactc tcatctggag catctgtggc tgattgttgc acaaaatcat
       61 gtattacagc gtcttttatt atggctcgat gcgaaatagg agaattaagt accattccat
      121 ttaatgtgcg acaacgacta agtgccacgt atgtttgtcc gtgagcaaac gaggaagcaa
      181 tatctataat cgctttatca aatgttagtc cttggctttt gtgaatcgtg attgcccaag
      241 ccaagcgaat aggatattgc tcaaaagtgc cttctatatc ttcgctaatc tcattagttt
      301 ctttgttcaa tacgtatttt gcatttgtcc atacggcttt ttgcaaacta aatggttcgg
      361 ggtcatcgtt tgtttgcact tcgataacat gctcatcgac atgtgttacc ttgcctattt
      421 ttccgttgta aaagcgtttt ggaaagctta catcgttttt gataaacata atttgagctc
      481 cctctttgat agtcaacaac tcgtcagcag gataagcatg ttgcgggaaa tttccattta
      541 cttgtgcttt atacgaaaat gcaggactgc taatttttgc taaatgttgg tcgttgattg
      601 tttgtgcttg gtgattgtgt gtagttaagc gaatatactc ttcgcctgct ggaggaataa
      661 aatctggaat atagcgtttg tttaattctt caagcgtcgt ggaggtagct ttgttgtcgc
      721 gtatttggtt taataaatcc aaaaaatgtt tctcgctttg gcgatatacc tgttttagtt
      781 cgatggtaag ataagctgtt ttttgcaagg ctttgctgtt aaaaaaatag ctactggggt
      841 aatacgcttc tagcaatctt ctttcttctt cttttattac aggagatagt tggtgcaaat
      901 cgccaataag taacagttgt acgcctccaa aaggtacggt tgtgcgtctg tatcttctca
      961 atatggcatc tacactgtcc aacaaatcgg cacgtaccat gctcacctca tctattacca
     1021 ataaatctaa actgcgtatg atgtttattt tttctttgtt aaattggtat tttgtatctg
     1081 tggcaaattc tgtaccagga atatagggag caaaaggtag ttgaaaaaat gaatggatag
     1141 taacgcctcc tgcatttatt gccgcaatgc cagtaggagc aagtactacc atgcgtttgg
     1201 gcgaggttgt tttaagttga tgcaagaaag tggtttttcc tgtacccgct ttacctgtta
     1261 aaaacaagag agtgcctgtt tgcactacac atcgccacgc taattcaagt tctgtatttg
     1321 tttccattcg tatgcaaaaa tacgatatat ctttggtaaa cgagccatat tttatactgc
     1381 ttctgtattt tttattacac aagtgtgtta tcattcgcta gtattttgta tctttacacg
     1441 taagagatag ttgaacatag aggttttacg aataaagaat gaagatggaa agtactagtt
     1501 ttatgcaact tgctatagat ttagcgatag agaatgtaaa acagggcaaa ggcggaccct
     1561 ttggtgccgt agtagtaaaa gagggtaaaa ttgtaggtca agcagccaat tctgttactt
     1621 cgaccaacga tcctactgct catgccgaag taaatgcaat taggcaggct tgtgctcatt
     1681 taaaaacgtt cgatttatca ggctgtgagt tgtatgccag ttgcgaacct tgccctatgt
     1741 gtttggctgc catttattgg gcacgtatcg acaaagtata tgtttcggct actaaatacg
     1801 atgctgcgga ggttggtttt gatgatgctt ttatttacga agaatttgcc ttgcccaaag
     1861 aaaacagaaa agtactacaa gtacctctat tggtacaaga aggcaaagct ccatttaggg
     1921 catggaaaca gaaaatagac aaaattccgt attaacaaac ctggattatt tcttgtagac
     1981 ataaaaaata ggctgactca acataaaatg ggtcagccta ttacactttt tttaagggta
     2041 tggcttattt tataaatact ttttcgtttg gttgcacatc ttcggtaatc catgtatttc
     2101 cgcctattac agcattttcg ccaatgatga ttctacccaa aatagtggca ctggcataaa
     2161 taatcacatt gtctttgata atgggatgtc tgtctatgcc tttaattggg atattatttt
     2221 catcaacagg aaaactttta gcccccagcg ttaccccttg ataaatacgc acattgtttc
     2281 caataataca tgtttcgcca attaccactc ctgtgccgtg gtcaatggca aatgactcgc
     2341 caatgcgtgc accagggtga atatcaatac ctgtttcggc atgagccatt tcggtaatta
     2401 tgcgtggaat gataggtgtt tgtagtttgt gtaattcgtg tgcaattcgg taattggtaa
     2461 tagccctaag tcctggatac gaaataatga tttcaccgta acttttagca gctggatcca
     2521 ttttatacat tgcctctacg tctgtacgca acacttcttt tatacggggt aattgtttta
     2581 caaacgaaag ggtaatcgta aatgcttttt cgcgtttagc ttccaagtca atgtcttctt
     2641 ctttacaatc aaaacacaaa ccggcatata cttgttccca aagcatagta aataattttt
     2701 ccacatttac accaatatgg tatggcaagg ttgttttgtt gacactcgca ttgccgaaaa
     2761 aacctggaaa caaaatagaa cgtgttaatt tgatactatc tttcattatt gcccgtgacg
     2821 gaaatggttc gccctctttg ttggggtgaa acatattttc tgttgggcat aagcctgtta
     2881 actcgtcgat aacagtctta atatcggagg cgtaattctt gtttttcatc agtttacgtt
     2941 cttattctta ttgcagggtg ttgtaatata tcgattcgct ttttagtact tcgatggctt
     3001 tttttacaaa atcgtttgtt tcgtttatta tttcaaaata ttcgtttgtc gaccaaagat
     3061 tgcgtgcaat gattgctttt gcctgtattg ctaacaagtc tttcgtttcg ttccattctt
     3121 cttccgtaaa cgcgatttct tttgctaagg tttcttgttt tatttgggag agaacttctt
     3181 gtgtgtcaaa tttcaattta aacgcttcaa aagtactaaa ttgttttgat acaagagctc
     3241 tatttttttc agcatataca atacttattt ttgtaatgat atgattgcgt atcagttgac
     3301 ggtgcaaggt ggtatattta gttgtgtcag caggcacaaa aatatcaggc ataatgccac
     3361 ctccaccata tacagtacgt tgggtaacaa gggttttgta tgtaagtgaa tcggcaaatg
     3421 taatgctgtc agccgataca aactccccat gtttatagcg atcaagaata tcttcctcgt
     3481 atttttcttt tcctttttcg taaggacgtt gaatgctgcg tccagaaggg gtgtagtaac
     3541 gagctacggt aagcctaatt tccgaaccat cgggcatagg tattggacgt tgtaccaatc
     3601 ctttgccaaa cgtgcgtctg ccaatcacta ctcctctgtc ccaatcttgc aaagctcctg
     3661 ctacaatttc gctagccgaa gcagaatatt catctactaa tacgactact ttgccacttt
     3721 ccataagtcc attacggcga gttgtcattg tttgtttggc atgattggct ccttcagtat
     3781 acacaactgt acgtttattt gagagcagtt catctactat ttcaatcgca ctttgcaaat
     3841 agccacctcc atttccttgt aaatcaatga taagcgactc catgcctttc tttttcaaat
     3901 tttttatgcc atctaccact tcttggtggg tggtgctggc aaaacggttg actttgatat
     3961 atccaatttt attgtctacc atataaaaag catctacact aaaaattgga atacggtcgc
     4021 gaatgatagt aaacggaatt gattcgttgt ttcgcaaaat agttactttt acttgtgttc
     4081 ctttgggacc ttttaagcgt ttggtaatgt cgctagtttt catttttacg ccagcaatcg
     4141 tggtatcgtt aaccaagatt attttatcgc caggcaatat gcctagtttt tcggatggtc
     4201 cgccagttat tgattggatt actaccaagg tatcttctac catattaaac gatacgccaa
     4261 tgccatcaaa actaccttgt agtgcttctg tcgattcttt tacctcatct gctgataggt
     4321 aactagaatg agggtctagc tcatcgagca tgcctttgat agcccattcg gttagtttac
     4381 tagggtcaac tttatctaca taaaaattgg taatagctcc atacgaaaag ttgagttttt
     4441 gcatttcgaa ttgggctgat gtttgtgcaa aagtggcaaa ccaaccaaca agaaggagta
     4501 tagaaaggat aggagttttc atacagtatt ttttttcgta acaacaaagg tacgcaaaac
     4561 attttattta acagaaatag aaaagtagag aaatggttaa aaaagtagaa ctagtactat
     4621 ttgttcttta tcttaccgca attttgtaac attcgtttcc tatacacaca aggtagatac
     4681 ctgcttgcga aactggaaat acgctgtttt cgctgcgggt gtcgttatac aatgtgtttc
     4741 caacaatgtt gaatattctg atggatgttt cgtgttccaa caaaacagaa atgttgcgat
     4801 tttgtgcaaa tactaccttg ttatcgtttg atatagaggg tattgctact ggttcgcaag
     4861 tagcattgaa tagcagattt tttatttccc acgtaccagc catgcttgat gttgctatgt
     4921 atttaaaggc aaaacgcacg ttttctttca tccattggct agaaaggttg atgttgccag
     4981 agtttacgta tgtccagcta tttcccaatg gcattgtagg aatacttagt tgtgtccaat
     5041 tggcattttt tggatctcct tcggtatagt ttgttgaaat ccataaggtg tgattgctgg
     5101 ctcgctcgct tgcttcttga gcatagttaa gggcatgctc aaacgacaag gagcccgatt
     5161 ctttgtcgga taaatcaaaa gcaggcgaaa taagccaatc ttcgttcgga ttattcgtgc
     5221 tactggcata tcctgacacg tatgctccgt aaatatttcg ccaatgccat gtttgtgtgc
     5281 cttctacaga ataggggata aaatcaccca agctacttgc aaagctctcc gaataatcta
     5341 tcgcttgaca gtcagttgga ttgttgcttg tgaaacggaa cagtgcactt attggcgtat
     5401 gatcggaggt tgtgttgcgg taattgctta tcgtattggt ggcactagtc tcgcgtatgg
     5461 tgctattcgc aacataatgg ctagccagtt catttgtaat aatgatattg tcaatcacag
     5521 ggctactgta ataaggatcg gttaaggaag aggtaagact ttggtaattt tccgtatcgt
     5581 ccataaagtt tttgtacgga gaattggagt acgtagctgt ttgcgaacct attaaatagt
     5641 cgttgaaatc gcctgtaatt acaatcttgc ttgtattata gttcgtccca tctagtatgc
     5701 cttttagtcc ttcggaagct ccaagccttc ggctatagct ggtttcgtca ctcatggctt
     5761 tggcgtgtat attgatgatg gtaaacggaa tagtcgttga gccaatagtc aatacgaaat
     5821 catgtataac aggataacga ccattagtcc aattatagta gtaactgttt ccttggctat
     5881 ttgccccgtt actcaataga gaagaattaa caagttgtag gtgggatttt ttgtaaataa
     5941 tgccctgatt ttgaccgcaa ttagtgggag tagtagccac gatatctcct gcccattcgt
     6001 ttattccaag ttttttcact aaggtatcga tggttgcata cgtaatgctt gtacctactt
     6061 cttgcaaggc gatgatatcg ggattaatcg tttgaataag ggaaacaata ttgttcattt
     6121 gcccttcctc atcagagggt ccatttgtat cacaactaag ccattcagta ttgaaagtgg
     6181 taatttttaa gtcaacagtt tgtgctaata tagaaaccag aactacatgt agcaggatga
     6241 ataatgcaat cagtgggcgg aaaaagagga aggtagtagg cttattcata taggtatgct
     6301 attagggtaa accacaaatg tatgaaaaaa aataatatgt ctttcgatta aagtgttaaa
     6361 tagctcattt tcactgaata cccacgtatt gttataccaa aaaaccttgc ttttttcaag
     6421 agcaaggttt agagaaagaa ttagttaacc tcttacaact gcaccgcaat accaattttc
     6481 aacatacgtc cagcgatggg cacgccgcca taatcgtaat aatgttggtt gaggatattg
     6541 gagatttcgg catacatttt tgtgttattt tgttgccagt tgatgcgagt gtcgatggtg
     6601 gcaaatggag tataagctac tttgtttcca tctttattta catagttccc tttacggtct
     6661 tggtagctta ctacccaact ggcatctacc tgcttgctta ttccatgcac atattgcagg
     6721 ttaattttgt gttgcaggta gtcgagagca tatttcgaaa ttagctcgcc cgcatctttg
     6781 ttgagcagaa cgtaggcata cgatgcttgt atgt
//
LOCUS       DAOPUR010000013         6612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_11557_length_6612_cov_2.966448, whole genome shotgun sequence.
ACCESSION   DAOPUR010000013 DAOPUR010000000
VERSION     DAOPUR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11557_length_6612_cov_2.966448"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PKN90_00405"
     CDS             <1..680
                     /locus_tag="PKN90_00405"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNZ84778.1"
                     /translation="SLRDPNNKEKYIGSDENWEKSEQAIIESCKAKGLQAKVELGEAA
                     FYGPKLDFMVKDAIGRRWQLGTIQVDYNLPERFELEYTGADNQKHRPVMIHRAPFGSM
                     ERFVAVLIEHTGGKFPLWLTPEQVVILPISEKFNDYAKEVVNTLNMHDIRASVDERNE
                     KIGKKIRDNELKRIPYMVIVGEKEAENNELSVRKQGEGDKGTYSINDFAKLIVDEVNN
                     MTNKFLA"
     gene            701..1108
                     /locus_tag="PKN90_00410"
     CDS             701..1108
                     /locus_tag="PKN90_00410"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNZ84779.1"
                     /translation="MKKLLITLFLAVSTVVFSFAQNELPEKASIFLEAHFSNIQIQKV
                     SRVDIEQVQIFKVLLADKTQIEFDQTGAWTKINYKKSPKTISYLPETLQQEIESNKQI
                     KKVREIETDGYDIAIQLKNKKQVKGNRFGKIEK"
     gene            1220..1777
                     /gene="infC"
                     /locus_tag="PKN90_00415"
     CDS             1220..1777
                     /gene="infC"
                     /locus_tag="PKN90_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNZ84780.1"
                     /translation="MAKVFTPRPNRNFQKKEEAHKTNEAIHHPEVRLVGENVEPGIYT
                     LAQAKHIADELNLDLVEISPNAVPPVCRVIDYTKFLYQQKKKEKELKAKTAKVTVKEI
                     RFGPQTDEHDYNFKLKHAIKFLEDGDKLKAFVFFKGRSILFKEQGEVLLLRFANDLEE
                     YAKVDQMPLLEGKKMIIMLSPKKKK"
     gene            1805..2002
                     /gene="rpmI"
                     /locus_tag="PKN90_00420"
     CDS             1805..2002
                     /gene="rpmI"
                     /locus_tag="PKN90_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNZ84781.1"
                     /translation="MPKMKTNSGAKKRFALTGSGKIKRKHAFKRHILTKKTTKAKRAL
                     TSFGLVDKTDESNVKFLLALK"
     gene            2108..2458
                     /gene="rplT"
                     /locus_tag="PKN90_00425"
     CDS             2108..2458
                     /gene="rplT"
                     /locus_tag="PKN90_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNZ84782.1"
                     /translation="MPRSVNHVASRARRKKILKLTRGYYGARKNVWTVAKNTWEKGLQ
                     YAYRDRKTKKRNFRALWIQRINAAARLEGMSYSKFMGALHKANIDINRKVLADLAMNQ
                     PEAFKAIVKQVQKA"
     gene            complement(2548..5168)
                     /gene="alaS"
                     /locus_tag="PKN90_00430"
                     /pseudo
     CDS             complement(2548..5168)
                     /gene="alaS"
                     /locus_tag="PKN90_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
     gene            5400..6374
                     /locus_tag="PKN90_00435"
     CDS             5400..6374
                     /locus_tag="PKN90_00435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ84783.1"
                     /translation="MGKAKYHFNEHTLNYDRIDNTIRHRLIKIGQYLIGSILMGIVFF
                     VIFIFTVKSPNEKLLEAENKRISTQYELLSRQLDDAMEVLTNIQQRDDNFYRVVLQVD
                     SIPSSIRRGNYINSNRYQKLLRMRTSDLVIATSQKMDMVSRMLYIQSNSFDELVALAK
                     QEEKKLQHIPAIQPIPNKDLKRTASGYGWRIDPIYHTRRFHQGMDFSAPTGTKIFATG
                     NGTVIKAGWEQGYGNAVFINHGFGYVTVYAHMHQLKKGLRRGSKVSRGEVIGFVGNTG
                     KSTGPHLHYEVRLKGKPQNPQNYYFMDLSPEEYDQMIQISANYGRVFD"
     gene            6383..>6612
                     /locus_tag="PKN90_00440"
     CDS             6383..>6612
                     /locus_tag="PKN90_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNZ84784.1"
                     /translation="MEEATNNIQESKQKLYFSIKEVAELVDVNISAIRFWEQQFDMIK
                     PRKNDKGTSFYTREHIDEFLLIKHLKEEKGIT"
BASE COUNT         2270 a         1242 c         1094 g         2006 t
ORIGIN      
        1 tttcattgcg cgacccaaac aacaaagaaa aatacatagg tagcgacgaa aactgggaaa
       61 aatctgaaca agccattatt gaatcttgta aagccaaagg cttgcaagca aaagtagaat
      121 tgggcgaggc cgcgttttat ggtccaaaac tcgactttat ggtaaaagat gccattggtc
      181 gccgttggca gttaggcacc attcaggtgg actataacct acccgaacgt tttgaattgg
      241 aatacacagg agctgacaat caaaaacacc gtcctgtaat gattcaccgt gctccattcg
      301 gttcaatgga acgttttgta gctgtactta tcgaacatac cggaggaaaa ttccctttat
      361 ggttaactcc tgaacaagtt gttattttac caattagcga aaaattcaat gattatgcta
      421 aagaagtggt gaatacgctc aatatgcatg atatacgtgc ctcggtggac gaacgcaacg
      481 aaaaaattgg taaaaaaatt cgtgacaacg aattaaaacg cataccgtat atggtaatcg
      541 ttggcgagaa agaagctgaa aacaacgaac tatctgtacg caaacaaggc gaaggtgata
      601 aaggaaccta ttccatcaac gattttgcaa agctaattgt agatgaagtg aataacatga
      661 ctaacaaatt tttagcataa tcaattaata gactgaactc atgaaaaaat tactaataac
      721 cctattcctt gcagtaagta ctgtcgtttt tagttttgct caaaatgaat tacctgaaaa
      781 agcaagtatt tttctagagg ctcattttag caatatccaa attcaaaaag taagtcgcgt
      841 tgacatagaa caagtacaaa tattcaaggt tttattagca gataaaacac aaatagaatt
      901 cgaccaaaca ggagcttgga ccaagattaa ttacaaaaaa tcaccgaaaa ctatttcata
      961 cttaccagaa actctacaac aagaaataga gagtaataag caaataaaaa aggtaagaga
     1021 aatagaaaca gatggatatg atatagctat ccaacttaaa aataaaaaac aagtgaaagg
     1081 aaatagattc ggtaaaattg aaaaataaaa aaaccactat ttttttaacg ataaattgtg
     1141 atatatcata aaaaaagcct atatttgcgg gctgaaaaat aagcatttag taattattta
     1201 atttaggagg acgtttacta tagcaaaagt ttttacaccg agacccaata gaaactttca
     1261 gaaaaaagaa gaagcccaca aaaccaatga ggctattcat catcctgaag ttcgtctagt
     1321 aggagaaaac gtagagcccg gtatctacac attagcacaa gcaaagcata tagcggatga
     1381 acttaatctt gatttagttg agatttcgcc taatgcagta ccgccagttt gtcgtgtaat
     1441 tgactataca aaattcttgt accaacaaaa aaagaaggag aaagaactaa aagctaaaac
     1501 ggctaaagtt acagtcaaag agattcgttt cggtccacag actgacgaac atgattataa
     1561 ttttaaattg aagcatgcta ttaaattttt agaagatggt gataaactaa aggcttttgt
     1621 attttttaaa ggtcgttcaa ttttatttaa agaacaaggc gaagtcttgc tactacgttt
     1681 tgccaacgac ttagaagaat acgcaaaagt ggatcaaatg cctttattag aaggaaaaaa
     1741 aatgatcatt atgctttcgc caaaaaagaa aaaataattt tttaattttt aaatatacaa
     1801 cgtaatgcca aaaatgaaaa ctaactccgg tgccaaaaag aggtttgccc ttaccggatc
     1861 aggaaaaatc aagagaaaac atgcatttaa aaggcacatc ttgacgaaga aaactaccaa
     1921 agcaaaaaga gccttaacta gtttcggact tgttgacaaa acagacgaaa gcaatgttaa
     1981 atttttgtta gcacttaaat agtctacgtc aaagtttatt ttatttgtaa atcaattcag
     2041 tattaaccga atgaatagcc gaaagatcac ctattagtga cgctaccatt caaaaaaaca
     2101 gaagaaaatg ccaagatcag tcaatcatgt tgcttcgaga gcaagaagaa aaaaaatctt
     2161 aaaactaacc agaggttact acggtgctag aaaaaacgta tggacagtag ccaaaaatac
     2221 ctgggaaaaa gggttacaat atgcgtatcg tgaccgtaaa accaaaaaac gtaacttccg
     2281 tgcattatgg attcaacgta taaatgcagc tgctagatta gaaggaatgt cttattctaa
     2341 atttatggga gctctacaca aagcaaacat cgatatcaac cgcaaggttc ttgccgattt
     2401 agctatgaac caaccagaag cattcaaagc tattgtaaaa caagtgcaaa aagcgtagtg
     2461 taattagaat aaagttttac tacttatata actaaaaaga ggaacaaaat aaattgttcc
     2521 tctttttagt tatatcgttt actcctacta agcagttatc gattcaataa tattttttac
     2581 agccgtatct atacccgaag ccctttttcc tcctgccgtt gcgtaaaatg gttgccctcc
     2641 tccacttcca tcaatatctg atgctatcga tttaatgatt gtagaagcat gtaatccttt
     2701 ttttactaaa ctatctgata acaacaacga tacacttacc ttgtcatcgt tttgtgtcgc
     2761 tgctagaaaa gcaaatggga tgtttgcaaa ctgtgattta aactgaaagg ctatatcttt
     2821 gcaaatttct gccgaatcat gcgacttaaa cacaataata tccacgccat taatttgctg
     2881 tacctgagtt aataacgttg ctttagtctg ttttacatac tcttgcacat agccatccaa
     2941 ctgttttttc aattgaagtg tgtcttgctg aaattttgtg attacaccta ccacatttgt
     3001 agggttatta aataactgtt taacttctgt aagtacatct aattgagctg taatatacgc
     3061 ttctgctttg tctgctgtat aggcttctat acgacggata cctgctgcta ccgaactttc
     3121 gtgcacaagt ttaatcattc cgatttctcc agttgattgt acgtgcgaac caccgcataa
     3181 ttctaccgaa gtgccaaact ggataacacg tactttttcg ccatattttt cgccaaacaa
     3241 tgccattgct cccattttgt ttgcttcatc aacaggtaat tctctatatt cttgcagaga
     3301 tgcattattg cgaatctttt tatttacgat caattctact gtttttaatt cttcggcact
     3361 tactttttgg taatgcgaaa aatcaaaacg caatgcttca tccgacacaa acgaaccttt
     3421 ttgttctaca tgtgtaccca atacctcgcg taatgcctcg tgaagtaaat gggttgctgt
     3481 atggttagcc gcaattcttt tgcgtttttc cacatctacc actgctgtat aggtagcatc
     3541 aatagtttct ggtaattcta atgtaatatg taccgttaac ccatttcctt tttagtatca
     3601 acaatggcaa tttttttagt atctgcaaga atatagccag tatctcctac ttgaccgccc
     3661 atttctccat aaaacggtgt acgatcgaaa actaattgat acagagattt tcccttttgt
     3721 accattttac ggtaacgaag caattgtaca tccatttcag caaaatcata cccatcaaat
     3781 actgattctc catcgcgaac tacaatccaa tcacccgttt ctacactggc tgcatttctg
     3841 gctctttcgc gttgtttttg catttcttgt tcaaactctg ctacatctac cattacgtta
     3901 ttttccttgg ctatcaattc agttaaatca agaggaaaac catacgtatc gtacaataca
     3961 aaagcatctt taccacttat ggttgtttta cctgcaagtt tggcttcagt aattacttta
     4021 tcgagcaaac ggatacctgt ttctaaggta cgtaaaaatg attcttcttc ctctttaatc
     4081 actttctcta tcaacccttt ttgtgcttct aattctggat aagcacttcc catactatca
     4141 ataagtgctg gaagtaatgc atgcataaac gatgaacgtt gatttaaaaa cgtatatcca
     4201 tatcgcaccg ctctacgcaa aatacgacga ataacatacc ctgcttttgc attagaaggc
     4261 aactgaccat cggtaatcga aaaagcaatc gtacgaatat ggtcggctat tacacgcaat
     4321 gcaatatctt gttcgttatt ttccccataa gacacccttg ctaatgctgc cattgatttg
     4381 ataataggtt gaaatacatc cgtatcgtaa ttcgatgttt ttccttgtat tgccatacac
     4441 aaacgctcaa atcccatgcc agtatctacg tgtttggcag gtaataactc caaggaaccg
     4501 tctgctttgc gattaaactg cataaacacg aggttccaaa tttctatcac ttgtggatga
     4561 ctttcattta ctaattttac accaggtact tttagttttt cttctgctga acgtaaatca
     4621 acatgtattt cggaacatgg accacatgga ccagtatctc ccatttccca aaaattatct
     4681 ttttgactac cattaataat acggtcttgg ggtaaatatt gtgcccaata tgctgctgcc
     4741 tcgttatctc tttctaagcc ttcggctgga ttgccctcaa aaacagtaac gtacaatatt
     4801 tctggattaa tttttaacac ctctactagg tactcccatg cccaatcaat tgcatctttc
     4861 ttaaagtaat cgccaaaact ccaattaccc aacatttcaa acatggtgtg gtgataagta
     4921 tcgtgcccta cctcttccaa atcgttgtgc ttgccactta cacgcaaaca tttttgcgaa
     4981 tttgctacac gtggatatac gactgtagca tttcccaaaa taatatcttt aaattggttc
     5041 atccccgcat ttgtaaacat gagtgttgga tcattcttaa ttaccatggg tgctgacgga
     5101 acaatcttat gtcctttgga tgcaaaaaag gatgtaaacg attctctaat ttctttggca
     5161 gatttcatta ttatttttca attaaaattt atcaaatcaa acatacgtat ctagtataca
     5221 aactaaatta taccaagcag ttagatattg gtatctaaaa taatttgcaa attcagattt
     5281 caagccacaa aattagtatt ttttttgtta tttttgttgt cttaaatgaa ttttcaaact
     5341 acttttgtca atctgtttgt cctgtgtaat aaacagattg gttttaggaa acaacaagca
     5401 tgggaaaagc aaaatatcat tttaacgaac atacccttaa ctacgatcga atagacaata
     5461 caattaggca ccgtctgatt aaaattggac aatatttaat cggaagcatt ttaatgggca
     5521 tcgttttttt tgttatcttc atttttacgg tcaaatctcc taatgaaaag ttactagaag
     5581 ctgaaaataa gcgtattagc acacaatacg agttactttc taggcaatta gatgatgcta
     5641 tggaagtgct aacaaatatt caacaacgtg atgacaattt ttatcgagta gtactccaag
     5701 tggattctat tccttcgagt atccgacgtg gaaattatat caacagcaat cggtaccaaa
     5761 aacttcttag aatgcgaacg tctgatttag tcattgctac cagtcagaaa atggatatgg
     5821 tatcaagaat gttatatatt caatctaatt cttttgacga acttgtagca ttagctaaac
     5881 aagaagaaaa aaaactacaa catattccag ctatacaacc cattcctaac aaggatttaa
     5941 aaaggacagc ttctggctat ggatggcgaa ttgacccaat ttatcataca cgtagatttc
     6001 atcaaggaat ggatttttcg gcaccaacag gtacaaaaat ttttgctact ggcaatggta
     6061 ctgttatcaa agctggatgg gaacaaggat atggaaatgc tgtatttatc aaccatggtt
     6121 ttggctatgt tactgtttat gcacatatgc accaactcaa aaaagggcta cgtagaggca
     6181 gtaaggtaag tagaggtgaa gtaattggat ttgtaggaaa tacaggtaaa tcaacaggtc
     6241 ctcacctaca ttatgaggta cgcttgaaag gaaaacctca aaatcctcaa aactattatt
     6301 ttatggattt atctccagaa gaatatgatc aaatgataca aatatctgct aattatggac
     6361 gtgtatttga ttaactctac taatggaaga agctactaac aacatacaag aaagcaaaca
     6421 aaaactatac ttctccataa aagaagtagc tgaattagtg gatgttaata tttctgctat
     6481 acgtttctgg gaacagcaat ttgatatgat taagcctcga aaaaacgata aaggaacaag
     6541 tttttatacg cgagaacaca ttgacgaatt cttactcatc aaacatttaa aagaagaaaa
     6601 aggaataaca tt
//
LOCUS       DAOPUR010000014         6370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_12140_length_6370_cov_3.362945, whole genome shotgun sequence.
ACCESSION   DAOPUR010000014 DAOPUR010000000
VERSION     DAOPUR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12140_length_6370_cov_3.362945"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKN90_00445"
     CDS             <1..286
                     /locus_tag="PKN90_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ84785.1"
                     /translation="MDAASCIGCGACAAACKNGSAMLFVAAKVSQLALLPQGKVERAT
                     RAKAMVAKMDELGFGNCTNTGACEAECPKNISISHIARLNREFLSAKIKD"
     gene            complement(357..716)
                     /locus_tag="PKN90_00450"
     CDS             complement(357..716)
                     /locus_tag="PKN90_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ84786.1"
                     /translation="MAAFQPLPFMAGYAASKAFVLSLSQALNEELKKQNVYVSTLCPG
                     VTESKFHNTANTEKKLLGASFFPVATAQEVAQYGYKLLMSGKNIGIYGLLNRIQIGLL
                     RFIPRAFIVKIVGIMMK"
     gene            complement(728..1126)
                     /locus_tag="PKN90_00455"
     CDS             complement(728..1126)
                     /locus_tag="PKN90_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ84787.1"
                     /translation="MKTVLITGASEGIGYELSQLFANAHYQLILVARNEQKLNAMCET
                     YTSNGVKCTYFVADLSIMSHVEKLADKIDQLPFKIDYLINNAGFGIYGNSTELDWEKE
                     SAMYQLNMISLAYLTKHFSIQMTKNGFGRI"
     gene            1284..3251
                     /gene="dnaG"
                     /locus_tag="PKN90_00460"
     CDS             1284..3251
                     /gene="dnaG"
                     /locus_tag="PKN90_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNZ84788.1"
                     /translation="MIDQVTVDRILQTAQIEDVVGEFVSLRKRGVNMIGLCPFHDEKT
                     PSFTVSPAKNICKCFGCGKGGTPVNFIMEHEHLSYYDALRYLAKKYNIEIVEKEISAE
                     ELNKRNERESLFVINTFAQTYFEDVLWKQDEGRSVGLSYFKERGFRDDVIKKFGLGYS
                     LESYDSFGRVALEKGYKRDYLLKTGLCTERQDASLVDRFRGRVIFPVHTLSGKIVAFG
                     GRILKTDEKTAKYLNSPESEIYHKSNELYGLFFAKQSIVKYDNCFLVEGYTDVLSMYQ
                     SGIENVVSSSGTALTPGQIRLIRRFTSNITVLYDGDAAGIKASIRGIDLLLEEGMNVK
                     VVLLPQGEDPDSFAKKQNASDFIQFIEKNQTDFIHFKSHLLLEEAGKDVSKKAKLIAD
                     IVQSIAIIPDAIMRSVYVKECAKQFEMNENVLFTEVNKRRILLLESQSKQTNFVVESE
                     DADNAISELPSDHSTVNAFLHYTFLQEQNLIACILRNGASKIVGQDENKAEYIISVFD
                     FILNDLAVDDLDFSYSLHQQICNELIRLRASNQEQIAFYFLNHPNKEIRDYVFELITD
                     KHTLSKVHQKYKAIESEQDRLNYQVTKYLLDFKEKIVDYYCKELRDKIKQSEGKVSDE
                     EMFSLLQELNRYNAIRNIIMKQSGEKTVIKI"
     gene            3270..4724
                     /locus_tag="PKN90_00465"
     CDS             3270..4724
                     /locus_tag="PKN90_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNZ84789.1"
                     /translation="MHYTIEDLIKLPIWQHFYAISQIPRPSGRMDAITDYIEGFGKSL
                     RLETLRDKANNIVIRKPASAGYEGCKKVILQSHLDMVPQKNEGVLHDFCTDAIKWKIT
                     DNWLMATDTTLGADNGIGVAAMMAILENKELVHPPLEALFTVDEENGMNGAFGVAPDW
                     LQGDILINLDSEDEQEIIVGCAGAVRVQCNFNFEKTPVPEGDKAYKIGIKGLLGGHSG
                     IDIHLQRANANKLLFHFLKTLVVDFEARVAYIKGGTIINAIPREAEAIITIPTGGEDD
                     LMSVYHDFKAIYTQEYSHIDPDVSIEIESIILPDGLLPEPIQDDIINAVIGAPNGVYR
                     YVPLSNWVETSTNLGIIETSTNCVSTHFLVRSNVDSMKGEVRSMIESIFSLAGAKISV
                     SGNYDGWSPKSSSNLVNLVREQYEHFFDRDPEVKTIHAGLECGIIGSKYPHLDMISIG
                     PTIKYPHSPNEKVDILSVDRFYRFLKVLLSQIGV"
     gene            complement(4725..5657)
                     /gene="ribF"
                     /locus_tag="PKN90_00470"
     CDS             complement(4725..5657)
                     /gene="ribF"
                     /locus_tag="PKN90_00470"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNZ84790.1"
                     /translation="MKIVTDLHTSLQQKTAATVGFFDGVHTGHVTLLNRLKKEASQRN
                     LASMVVTFATHPREILQSNYQPELLTTTEERITLLAKNNIDICVLLPFSKEMSTLTSE
                     QFMKRILKETLLTEYLLLGHDHQFGSDRQNGAIEYIQFGKIIGMKVESETAYMKGNTC
                     ISSSTIRRALATHKIEEANTYLNYPYFISGIVIGGKQIGRTIGFPTANISLTDKRKLI
                     PADGVYAVKVTIKGNIHQGMMNIGTRPTIDQSLQKTLEVHILDFHESIYQENISIEFL
                     SFLRNEMRFDSLEKLVQQLLIDKENTITFFQQHT"
     gene            complement(5664..6275)
                     /locus_tag="PKN90_00475"
     CDS             complement(5664..6275)
                     /locus_tag="PKN90_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNZ84791.1"
                     /translation="MIKNIVFDMGGVLINLDKNRCIAAFNKIGFNDVELYIDKYIQNG
                     LFLSYEKGEIDTNVFLENIQQHIGKQVDFTEITNAWCAILLDIPTYKLTLLRMLRKKY
                     RTFMLSNTNAIHFERITQDYFEKDGYSIPHYFEKCYLSYQMNCVKPHNQIYLTMLHDA
                     QILASETLFIDDSVENIESAKKLGFQTYLAQEQEDFSHLFVSL"
BASE COUNT         2055 a          920 c         1267 g         2128 t
ORIGIN      
        1 aatggacgct gcctcttgta tcggttgtgg tgcttgtgca gcggcttgta aaaatggatc
       61 agcaatgcta tttgttgctg caaaggtaag tcaacttgca ttattgcctc aaggaaaagt
      121 agaaagagca acacgagcca aagcgatggt tgctaaaatg gatgaattag ggtttggtaa
      181 ttgtactaat acaggagctt gtgaagcaga gtgtcctaaa aatatttcca taagccatat
      241 tgctcgttta aacagagagt ttttgtcggc taagataaaa gattaaccgc taggtttgtc
      301 aataaaaaag ctgctttgta attcacaaag cagctttttt attgaacgaa gtcaatttat
      361 ttcatcatta tcccaacaat ttttacaata aatgcacgag ggataaatcg taacaagcca
      421 atttgaatgc gatttagcaa gccatatatg ccgatgtttt tgcctgacat aagtagtttg
      481 tacccatact gagcaacttc ttgtgctgtg gcaacaggga aaaaggaagc tcccaagagt
      541 tttttttctg tattggctgt gttatgaaat ttactttctg taacacctgg acacagggta
      601 gacacatata cgttttgctt tttaagttct tcgttcaatg cttgagataa gctaagcaca
      661 aaagctttgg aggccgcata ccctgccata aatggcaaag gttgaaaggc ggcaatagag
      721 gcaacattta aatccttcca aaaccatttt ttgtcatttg aattgaaaag tgttttgtta
      781 aataggctaa actaatcata tttagttgat acatagcaga ttctttttcc caatccagtt
      841 cagtagagtt tccatatatc ccaaacccag cgttgtttat tagatagtct attttgaatg
      901 gcagttggtc gattttatct gcaagctttt ccacgtgaga cataatggat aaatcagcca
      961 caaaatacgt gcattttaca ccattagatg tatatgtttc acacatggcg tttaattttt
     1021 gttcatttct tgccaccaaa ataagctgat aatgtgcgtt ggcaaataat tgagacaatt
     1081 cgtatcctat accttctgat gcccctgtta taagtacggt tttcataagt tttgaagttt
     1141 atttattact cgatattctt gttgcgataa atttttgttg agatgcccta caaatatacg
     1201 agattttttg tagaaaaaag ctcctacatc aagatagata aatagtaaaa ttctcgtata
     1261 tttgtgttcc taacaaaata gagatgattg atcaagtaac agtggatagg attctacaaa
     1321 cagcacagat tgaagatgtt gttggcgagt ttgtatcact tcgtaaaaga ggggtgaata
     1381 tgattgggct ttgtccattt catgacgaaa aaacgccatc gtttactgtt tctccagcca
     1441 aaaatatttg taaatgtttt ggttgtggaa aaggaggaac gcctgtaaat tttatcatgg
     1501 agcatgagca tttatcatat tatgatgcgt tgcgttactt agctaaaaaa tataatattg
     1561 aaatagtaga aaaggaaata tcggcagagg agttaaataa acgcaacgaa agagaaagtt
     1621 tgtttgtaat taacactttt gctcaaactt attttgagga tgttctttgg aaacaagacg
     1681 agggaagatc cgtgggttta tcttatttta aagaaagagg ttttcgtgat gatgttataa
     1741 agaaatttgg cttaggttat agtttggaat cgtatgattc ttttgggcgt gtagctttag
     1801 aaaaaggcta taagcgggac tatcttttaa aaacaggatt gtgtactgaa cgtcaagatg
     1861 cgagtttggt tgatagattt cgaggaaggg tcatttttcc tgtccacaca ttgtcgggaa
     1921 agattgtggc ttttggaggt agaattttga agactgatga aaaaacagca aaatacctca
     1981 attcaccaga atctgaaatt tatcataaaa gtaatgagtt gtatggattg ttttttgcta
     2041 agcaaagtat tgtaaagtat gataattgtt ttttggtaga aggatataca gatgtgcttt
     2101 ctatgtatca aagtgggatt gagaatgttg tatcatcttc aggaaccgct cttactcctg
     2161 gtcagattcg tttgatacgt aggtttacct ccaatattac ggtgttatac gatggagacg
     2221 ctgctggaat aaaagcgtct attaggggta ttgatttatt gctagaagaa ggcatgaatg
     2281 taaaagtggt gttattgcca cagggagaag acccagactc ttttgctaaa aaacaaaatg
     2341 ccagtgattt tatacagttt atagagaaaa atcaaacaga ctttattcat tttaaatcac
     2401 acttgttgct tgaagaagcc ggcaaagatg tgagtaagaa agccaaacta attgcagata
     2461 ttgtgcaaag cattgcgatt ataccagatg ctattatgcg ttctgtatat gtaaaagagt
     2521 gtgctaaaca atttgaaatg aatgagaatg ttttgtttac agaagtaaat aaaaggcgga
     2581 ttcttttgct tgaatctcaa tcgaaacaga caaattttgt ggtggaatct gaggatgctg
     2641 ataatgctat tagcgaactt ccttcagatc attcaacagt gaatgcattt ttgcattaca
     2701 cttttttgca agaacaaaat ctaatagcct gcattttacg aaatggagca tctaagattg
     2761 taggtcagga tgaaaacaag gcagagtata ttatttcagt ttttgatttt attttaaatg
     2821 atttagcggt tgatgattta gatttttcct attctttgca tcagcagata tgtaacgaat
     2881 taattagatt gagagcaagc aatcaagaac aaatagcatt ttatttttta aatcatccaa
     2941 ataaagaaat tcgggattat gtctttgaac taattacgga taaacatact ttgagcaaag
     3001 ttcatcaaaa atacaaagct attgaatcgg agcaagatag attaaattat caggtaacca
     3061 agtatttact tgattttaaa gagaaaattg tagattatta ttgcaaagag ttgagagata
     3121 agataaaaca atccgaaggg aaagtgtcgg atgaagagat gttttcttta ttgcaagaat
     3181 tgaatcgata caatgcaata cgaaatatca taatgaaaca atctggcgaa aaaacggtca
     3241 taaagattta actataactt ttttaattca tgcattatac aatagaagat ttgataaaat
     3301 tgccaatatg gcaacacttt tatgcaatta gccaaattcc acgtccatct ggtaggatgg
     3361 atgccatcac tgattatatt gaagggtttg gaaaatcatt gcgtttagaa acgctacgag
     3421 acaaagcaaa taatattgta atacgaaaac cagcaagtgc aggatatgag gggtgcaaaa
     3481 aggtcatttt gcaaagccat ttggatatgg tgccacaaaa aaatgaaggg gtattgcatg
     3541 atttttgtac tgatgctatt aagtggaaga ttactgataa ctggttgatg gcaacagata
     3601 caacccttgg ggcagataac gggataggag ttgcagcaat gatggctatt ttagagaata
     3661 aagaacttgt gcaccctcca ctagaggcat tgtttacagt agatgaagaa aatgggatga
     3721 atggagcttt tggagtagcc cctgattggt tgcaaggaga tatacttatt aatttagatt
     3781 cggaagacga acaagaaata atagttggat gtgcaggagc agtgcgagta caatgtaatt
     3841 ttaattttga aaaaactcct gttcctgaag gagataaagc atataaaatt ggaataaaag
     3901 ggcttcttgg agggcattca gggattgata ttcatttgca acgtgctaat gccaataaat
     3961 tattatttca ttttttaaag accttagtgg ttgattttga agcaagagtt gcctatataa
     4021 aaggagggac tatcattaat gctattccac gtgaggcaga agctattatt acaattccta
     4081 caggaggaga agatgatttg atgagtgtgt atcatgattt taaggctatt tatacacaag
     4141 aatatagcca tattgatcct gatgtatcaa tcgaaattga gtccattata ttacctgatg
     4201 gattattgcc agaacctatt caggatgata ttatcaatgc tgtaataggg gctcctaatg
     4261 gggtatatcg ctatgtccct ttgtctaatt gggtggaaac ttctacaaat ttggggatta
     4321 ttgaaacttc tacaaattgt gtgagtaccc attttttagt aagaagtaat gtggattcta
     4381 tgaaaggaga agtgcgatcg atgatagaaa gtatctttag tttggctggg gcaaaaatta
     4441 gtgtctcggg gaattatgat ggatggagcc ctaaatcatc atctaatctt gtcaacttag
     4501 tacgagaaca atacgaacat ttttttgata gagatcctga ggtaaagaca attcatgcgg
     4561 gtttagagtg tggcattatt ggaagtaaat atcctcattt ggatatgatt tcgataggtc
     4621 caacaattaa ataccctcat tctcccaatg aaaaagtaga tatactaagt gtagatagat
     4681 tttatcgttt tctgaaagtt ctcttgtctc aaatcggagt ataattaggt atgctgttga
     4741 aaaaaagtga tcgtattttc tttgtcaata agtagttgtt gtactagttt ttccaatgaa
     4801 tcaaatctca tttcgttgcg taagaaagaa agaaactcaa tggagatatt ttcttgataa
     4861 atggattcat ggaaatcaag aatatgtact tcgagtgttt tttgaaggga ttgatctatg
     4921 gtaggacgag tgcctatatt catcattcct tggtgtatgt ttcctttaat agtaaccttt
     4981 acagcgtata ctccatctgc aggaattagt ttgcgtttat ctgtaagaga tatatttgca
     5041 gtcggaaagc caatagttct tccaatttgt tttcctccaa tgacaatgcc acttataaaa
     5101 tatggataat ttagatacgt atttgcttct tctattttat gtgttgctag agccctgcgt
     5161 atggtggaag aactaataca agtatttcct ttcatataag ctgtttcgct ttctactttc
     5221 attcctatta tttttccaaa ctgtatgtat tctatagctc cattttgtct atctgaccca
     5281 aattgatgat cgtgtccgag caatagatat tctgttagaa gagtctcttt gagtatccgt
     5341 ttcataaatt gttcagaagt tagtgtggac atttctttgc taaaggggag caatacacat
     5401 atgtcgatgt tgtttttagc taataatgtt atacgttctt ctgtagttgt tagtaattcc
     5461 ggttgatagt tgctctgcaa tatttcgcgt ggatgtgtag caaacgtaac gaccatacta
     5521 gctaagtttc gttgggaagc ttccttttta agcctattta agagggttac atgacctgtg
     5581 tgtacaccat caaagaaacc aacagtagca gctgtttttt gttgtaaaga agtatgtaaa
     5641 tccgtaacta ttttcataat gaactacaaa gatacaaaca aatgagagaa atcttcctgt
     5701 tcttgagcta aataggtttg aaaaccgagt ttttttgctg attcaatatt ttctaccgaa
     5761 tcgtcaataa ataatgtttc actagcaagt atctgtgcat catgtagcat cgttagatat
     5821 atctgattat gaggttttac acagttcatt tgataagata gatagcattt ttcaaaatag
     5881 tgaggtatag aataaccatc tttttcaaaa taatcctgtg taattctttc aaaatgaatc
     5941 gcattggtat tgctcaacat aaatgtgcgg tatttttttc ttagcatacg caataaagtt
     6001 aatttatagg taggaatatc aagtaaaatg gcacaccaag catttgttat ttcagtgaaa
     6061 tcaacttgtt ttcctatatg ttgttgtatg ttttctaaaa atacatttgt atcaatttct
     6121 cctttttcgt agcttaagaa tagtccattt tgaatgtatt tgtcaatata gagttctaca
     6181 tcattgaacc ctattttgtt aaaagcagcg atacaacgat ttttatctaa gttaatgagt
     6241 accccaccca tgtcaaatac aatatttttt atcatttttc aaaacgaata ttttgttgtt
     6301 atacaaaaaa aaattacttt tgtactcgct tctattgagg cgattttatg agaatatttt
     6361 tgggcctata
//
LOCUS       DAOPUR010000015         6301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_12327_length_6301_cov_3.220301, whole genome shotgun sequence.
ACCESSION   DAOPUR010000015 DAOPUR010000000
VERSION     DAOPUR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12327_length_6301_cov_3.220301"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PKN90_00480"
     CDS             complement(<1..1145)
                     /locus_tag="PKN90_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNZ84792.1"
                     /translation="MSNSSLKDKMIGALAWSSVDRVAQQGIQFIFGIILARLLAPDDF
                     GLLGMIMIFATLSYVLVESGFSAALIRKEDANETDYNSVFYVNLAISVALFVLLFFTI
                     PYFAEFFKQPALVSTARITFIAVIFNAFYLVPYVQVNRAMDFKTLAKINLFATLCSGS
                     IGITLAILDYGVWALVYQQIGYQFFRGIGFYALVRWIPSFTFSLDSIKHLWKFSMHIL
                     GSSLLTIGFNNLYTFLLSRFYPIKQVGYYTQANKMSETANFTFQAILASSTYNLFTQI
                     QADKERLLRVYRQLIQHISLVVIPVSLVLMVVAKPLFFVLFSEKWLPAVPYFQLLCVA
                     NVFAPLYLVNMNVLNSLGESKRTFYIEIIKRLFILISIVVCFQFGIQA"
     gene            complement(1168..3924)
                     /gene="leuS"
                     /locus_tag="PKN90_00485"
     CDS             complement(1168..3924)
                     /gene="leuS"
                     /locus_tag="PKN90_00485"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNZ84793.1"
                     /translation="MEYTFKEIEQKWQQYWKNNATYKVEINATKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIFSRFKRLKGYNVLHPMGYDAYGLPAEQYAIQTGQHRAITTTK
                     NIARYREQLDKIGFSYDWNREVRTCEPDYYKWTQWAFVKMFNHYYDTQLQQAKPIENL
                     VKHFATQGTDSLQAACTEELSFTAKEWNEKTDEEQQHILLNYRIAYLADLKVNWCPAL
                     GTVLANDEVSEGVSIRGGHPVEQRIMRQWSLRISAYAQRLLDGLDTIDWSESIKETQR
                     NWIGRSEGAEMKFPIKGQDADLLIFTTRADTTYGVTFMVLAPESEYVQQLTTVEQQQA
                     VETYIESVKKRTERERIADRKVSGVFTGSYAINPFSQQEIPIYISDYVLAGYGTGAIM
                     AVPAHDSRDYAFAKHFNLPIVPVIEGCDVSEESFDAKDGVMCNSGFMNGMTVKAAIET
                     AKKYVSDNGIGRVKVNFRLRDAIFSRQRYWGEPFPVYYKNGLPYMLDESKLPLELPEI
                     DKYLPTETGEPPLGRAKAWHTAEGYPYELNTMPGFAGSSAYFLRYMDPRNSNELVSKE
                     ANNYWQQVDLYLGGTEHATGHLIYSRFWNKFLFDLGLICKDEPFKKLVNQGMIQGRSN
                     FVYRIKDTNTFVSYHLKDQYEVTPIHVDVNMVSNDILDIERFKAWNPEYNTAEFILED
                     GKYVCGWAVEKMSKSMFNVVNPDDIVEKYGADTLRLYEMFLGPLEQSKPWDTNGIDGV
                     HRFLRKLWSLFYKDGNYIVTQDTPTTEELKTLHKTIKKITFDIENFSFNTSVSAFMIC
                     VNELAQQKCSKQAILEPLVILIAPFAPHIAEELYHALGHTTTVCDAVFPICNESYLVE
                     STINYPISFNGKVRFTLPLEASLSKEEVEKAALQNEQTRNFLQDKQPKKIIVVPGKIV
                     NIVL"
     gene            4098..5189
                     /gene="recF"
                     /locus_tag="PKN90_00490"
     CDS             4098..5189
                     /gene="recF"
                     /locus_tag="PKN90_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677365.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNZ84794.1"
                     /translation="MVLTKLSIVNFKNIADASLDFCAGINCFIGNNGMGKTNLLDAIY
                     YLSFCKSHTNAIDSQNVKHDEGFLMLQGNYIHQQQEEEIYVGLKRGQKKLFKRNKNAY
                     ERISDHIGLIPLVLVSPSDAVLIDGGSDERRKFIDGALSQYDKKYLDCLLKYNHVLQQ
                     RNALLKSNAVEETMYEVCEMQMAHFADYIYAQRTQFISQFLPVFQQYYVAIANVNEQV
                     SISYKSHIEDGDLTIQLKQSRAKDTLLGFTTKGIHKDELEFYLGDYLVKKVGSQGQNK
                     TFLVALKLAQFEFLKQVHACNPLLLLDDIFDKLDANRVSKIIQMVSGDNFGQIFITDT
                     NREHLVSILQQLPQENRIFLVNEDGINKE"
     gene            5196..5486
                     /locus_tag="PKN90_00495"
     CDS             5196..5486
                     /locus_tag="PKN90_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNZ84795.1"
                     /translation="MRRQNTEKIDDVIKQIFQQQNLQPKLDEVDILQSWETVLGPTVK
                     KYTSDLVIKNKVLYVKLSSSVLRNELMMSKSRLLQALNDTVGKEVITDIVLR"
     gene            5591..6166
                     /locus_tag="PKN90_00500"
     CDS             5591..6166
                     /locus_tag="PKN90_00500"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ84796.1"
                     /translation="MTFELKKLPYDPTTLNPVVSPKTFEFHHGKHLQAYVNNLNNLKT
                     GTKFENMDLISIIKESEGPIFNNAAQIFNHEFYFDQFAPSPKSKPEGALAAAIDAAFG
                     SFDNFKKEFSAAAVSVFGSGWAWLVVNAAGKLEITKESNAGTPLTKNQKPLLTFDVWE
                     HAYYIDFQNRRPDHVAALWDVIDWKVIESRF"
BASE COUNT         2031 a         1152 c         1130 g         1988 t
ORIGIN      
        1 gcctgaatgc caaattgaaa acaaacaacg atagagataa gaatgaatag tcgcttgatt
       61 atttcaatat aaaaggtgcg tttcgattcg cccaatgaat ttagtacatt catatttact
      121 aaatacaagg gagcaaaaac attggctaca cacaacaatt gaaaataagg cacggcaggc
      181 aaccattttt ctgaaaatag tacaaaaaac aaaggtttag caactaccat taatactaga
      241 gatacgggta ttaccacaag cgatatatgt tgtatcagtt gtctgtacac acgaagtaat
      301 cgttctttgt cagcttgtat ttgtgtaaat aagttgtacg tactacttgc caaaatagct
      361 tgaaaggtaa aattcgctgt ttcactcatt ttatttgctt gtgtataata tcctacttgt
      421 ttgataggat aaaaacgact taataaaaag gtgtataaat tattaaaacc aattgtcagc
      481 aatgacgagc ccaatatgtg catgctaaat ttccataaat gtttgatgct atccaaagaa
      541 aaagtgaaag agggtatcca tctcaccaaa gcataaaacc caattcctct aaaaaattga
      601 tagccaattt gttgatatac taatgcccat acgccataat ccaatattgc caaagttata
      661 ccaatgctac cactacaaag ggttgcaaaa agattgattt tagccaaagt tttaaaatcc
      721 attgctcgat tcacttgcac gtatggaacc aagtaaaaag cattaaaaat gaccgcgata
      781 aatgtaatgc gagcagtaga aaccaatgca ggttgcttaa aaaattcagc aaaatagggt
      841 atagtaaaaa acaaaagcac aaacaatgca acagaaattg ccagattgac ataaaaaacc
      901 gaattgtaat cggtttcgtt ggcatcttct ttgcgaatta gggcagcact aaaaccgctt
      961 tctaccaaca catacgagag ggtagcaaat atcataatca ttcctaacag accaaaatca
     1021 tcaggagcca aaaggcgggc aagtatgata ccaaaaataa attgaatgcc ttgttgagca
     1081 actctatcta cagagctcca cgccaaggca ccaatcattt tatctttcaa actactgttg
     1141 ctcatttagc ttttggttag gcggtcgtta tagtactata tttacaattt tacctggtac
     1201 cacaataatt tttttgggct gcttgtcttg caaaaaatta cgtgtttgtt cgttttgcaa
     1261 ggctgctttt tcaacttctt cttttgatag agaagcctct aagggcaagg taaaacgtac
     1321 ttttccatta aatgaaatag ggtaattgat agttgattct accaagtaac tttcgttgca
     1381 aattggaaaa acagcatcac aaacagtagt tgtatgcccc aaagcgtgat ataattcttc
     1441 tgcaatgtgc ggagcaaatg gagctattaa aattaccaat ggttctaaaa tagcttgttt
     1501 gctgcatttt tgttgtgcca attcgtttac acaaatcata aatgccgaca cagaggtgtt
     1561 aaacgaaaaa ttctctatat caaaggtaat ttttttaatc gttttgtgca aggtttttag
     1621 ttcttctgta gttggtgtat cttgcgttac aatatagttt ccatctttgt aaaacaacga
     1681 ccataatttg cgtaaaaaac gatgaacacc gtcaatccca ttggtatccc aaggtttgga
     1741 ttgttccaac ggacccaaaa acatttcgta caaacgcaag gtgtcagcac catatttttc
     1801 tacaatatca tcagggttta ctacgttaaa cattgatttt gacatttttt ctactgccca
     1861 accgcacaca tattttccat cttcaagaat aaattcagcc gtgttgtatt ctggattcca
     1921 tgctttaaat cgttcaatat caagtatgtc gttgctcacc atattcacat ctacgtgtat
     1981 tggggtaact tcatattggt cttttaggtg atatgaaaca aaggtgttgg tatcttttat
     2041 gcgatacaca aaattacttc gtccttgtat catgccttga ttgactaact ttttaaaagg
     2101 ctcatcttta caaattaatc ctaggtcgaa taaaaatttg ttccaaaaac gactataaat
     2161 taaatgccct gtagcgtgtt ctgtaccacc caaatataaa tctacttgtt gccaatagtt
     2221 attggcttct ttgcttacca attcgttgct attacgagga tccatgtagc gtaaaaagta
     2281 cgccgaagaa ccagcaaaac caggcatggt atttagctcg tagggataac cttcggctgt
     2341 gtgccaagct ttagcccttc caagcggagg ttcgcccgtt tcggtgggta aatatttgtc
     2401 aatttcgggc aattccaaag gcaatttgct ttcatcaagc atataaggta agccattttt
     2461 gtaatacaca ggaaatggtt ctccccaata acgttgacga ctaaatatag catcacgcaa
     2521 acggaaattc acctttacac gaccaatacc gttgtcgcta acatattttt ttgcagtttc
     2581 aatggcagct ttcactgtca ttccattcat aaaaccggaa ttgcacatca caccatcttt
     2641 ggcatcaaaa ctttcttcac tcacatcgca cccttcgata acaggtacta tgggtaaatt
     2701 aaaatgtttg gcaaatgcat agtcgcgact atcgtgtgca ggcactgcca taatggctcc
     2761 tgtaccataa ccagctaata cgtaatcgct gatgtagata ggtatttctt gttggctaaa
     2821 tggatttatc gcatacgaac ccgtaaatac gcccgatact tttcggtcgg caatacgttc
     2881 gcgttcggta cgttttttta ctgattcaat ataagtttca acggcttgtt gttgctccac
     2941 tgtagttaat tgttgtacat actcactctc gggagcaagt accataaagg ttaccccata
     3001 cgtagtgtca gcacgggtgg taaaaatcaa caaatcagca tcttgtcctt taataggaaa
     3061 tttcatttcg gcaccttcgc ttctgccaat ccagttgcgt tgtgtttctt taatcgattc
     3121 tgaccagtca atggtatcca acccatcgag caatcgttgt gcataagcag aaatacgcaa
     3181 actccattgt cgcataatgc gttgttctac tggatgacct ccacggatag aaacgccttc
     3241 gcttacctcg tcgttggcta gcacagtgcc caaggcagga caccagttta cttttaagtc
     3301 agctaaatag gcaatgcgat aattcaacaa aatatgttgt tgttcttcgt cagttttttc
     3361 attccattct ttggctgtaa aactaagttc ttcagtacaa gcggcttgca atgaatctgt
     3421 gccttgcgtt gcaaagtgtt ttaccaaatt ttctatgggc ttggcttgtt gtaattgcgt
     3481 atcgtaatag tgattaaaca tttttacaaa agcccactgt gtccatttgt aataatcagg
     3541 ctcgcaagtg cgtacttcac gattccaatc gtaactaaat ccaatcttat ctagttgttc
     3601 gcggtagcga gcaatgtttt tagtggttgt aatggcacga tgctgacctg tttgtatggc
     3661 atattgttcg gcaggcaatc cataagcatc ataacccatc ggatgcaata cgttatatcc
     3721 ttttaggcgt ttgaatctac tgaatatgtc agatgcaata taccccagcg ggtgcccaac
     3781 atgtagtcct gctcccgatg gatacggaaa catatccaat acatagtatt ttggtttagt
     3841 agcgttaatt tctactttgt atgtggcatt atttttccaa tactgttgcc acttttgttc
     3901 aatttcttta aatgtatatt ccataatagc ttgtgagagt attttcagtt atttagggca
     3961 caaagttaat gaaaatacag tgctttccca tatttttcta tcattttata tgtataccac
     4021 ttccttcgta aacattgatt ctatttttta acgcattcaa tcaaactttt ttgctatttt
     4081 tgcataaaaa ttacagtatg gttcttacca aattatccat agtaaacttt aaaaatattg
     4141 cagatgctag ccttgacttt tgtgctggta ttaactgctt tatcggaaac aacggaatgg
     4201 ggaaaaccaa tctgttagat gctatttatt atctttcttt ttgcaaaagt catacaaatg
     4261 caattgatag tcagaatgtt aaacatgatg aaggcttttt aatgttgcaa gggaattata
     4321 ttcaccaaca acaagaagaa gaaatttatg tggggttgaa acgtggtcag aaaaagttat
     4381 ttaaacggaa taaaaatgct tatgaaagaa tatccgacca tatcggatta attccattgg
     4441 tgttggtttc gccaagtgat gctgtgttga tagacggtgg tagtgatgaa agacggaagt
     4501 ttattgatgg cgctctttct cagtacgata aaaaatacct tgattgtttg ctcaaataca
     4561 atcatgtgtt gcaacaacgc aacgcattgc ttaaatccaa tgcagtagaa gaaactatgt
     4621 acgaagtgtg cgaaatgcaa atggcacatt ttgctgacta catatatgct caacgtaccc
     4681 agtttatttc tcaattttta ccagtttttc agcagtatta tgtagctatt gccaatgtaa
     4741 atgagcaagt tagcataagc tacaagtcgc atattgagga tggtgattta accattcagc
     4801 ttaaacaaag cagagccaaa gatactctct taggctttac cacaaaagga attcataaag
     4861 atgaactgga attctattta ggagattatt tggtaaaaaa agttggttca caaggtcaaa
     4921 acaaaacctt tttagtagcc ctcaaattgg cacagtttga atttttgaaa caagtgcatg
     4981 cctgtaatcc cctattatta ttagatgata tttttgataa gttagatgca aatagagtaa
     5041 gtaaaataat acaaatggta tcaggagata attttggtca aatttttatt acagacacga
     5101 atagggaaca tttagtgagt attttacaac agttaccaca ggaaaatcgc atttttttag
     5161 taaatgaaga cggtataaac aaagaataac ctactatgag aagacaaaat accgagaaaa
     5221 ttgacgatgt tatcaagcaa atttttcaac aacaaaattt gcaacccaaa cttgatgagg
     5281 tggatatttt gcaatcttgg gaaacggtgt tgggaccaac tgttaaaaaa tacacaagcg
     5341 atttggtgat aaaaaacaag gtattgtatg tcaaattaag ttcttcagta ttgcgtaatg
     5401 aattgatgat gagtaaaagt cgccttttac aagcccttaa tgatacggtt ggtaaggagg
     5461 ttatcacgga tatagtacta agataaaata acataaaatt cttgctttca ttttgttttt
     5521 tcacttttta tacgtaaatt tgaaaatcta tttaaacaaa tttgttaaac aattttaaaa
     5581 acatacagtt atgacatttg aactaaaaaa attgccgtat gaccctacta cattaaaccc
     5641 agtggtaagt cctaaaactt ttgagtttca tcatggaaaa cacttacaag cttatgtaaa
     5701 taatctaaac aacctaaaaa cagggacgaa gtttgaaaac atggatttga tttctattat
     5761 taaagaatca gaaggtccaa ttttcaacaa tgctgctcaa atttttaacc acgaatttta
     5821 ttttgaccaa tttgcaccaa gcccaaaatc aaaaccagaa ggagctttag ctgctgctat
     5881 tgatgctgcc tttggctctt tcgataattt caagaaagaa ttttcggctg ctgcagttag
     5941 tgtgtttggt tcgggttggg catggctagt agtaaacgct gctggtaaat tagaaattac
     6001 caaagaaagc aatgctggta caccattaac aaaaaatcaa aaaccattac ttacttttga
     6061 tgtgtgggag catgcttatt acattgattt ccaaaacaga cgccccgacc acgtagcggc
     6121 tctttgggat gtaattgatt ggaaagtaat tgaaagtcgt ttttaataac acgaatactt
     6181 gtgtctacta tagcacaaaa tattgaattt cttagaaaac agctgccaaa tcatgtgcgt
     6241 ttggtggctg tttctaagtt taatcctgcc gaaagtgtga tggaagcata tcgggtagga
     6301 c
//
LOCUS       DAOPUR010000016         6258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_12434_length_6258_cov_3.407867, whole genome shotgun sequence.
ACCESSION   DAOPUR010000016 DAOPUR010000000
VERSION     DAOPUR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12434_length_6258_cov_3.407867"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PKN90_00505"
     CDS             complement(<1..1042)
                     /locus_tag="PKN90_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ84797.1"
                     /translation="MRKLYSKLFALMLVISGFLVPMQLQADVHKRVVLQAFWWNYWNE
                     NYPNSYANYLTELAPRLKEYGINAVWIPPSYKNTGTNSVGYSPFDHYDLGDKYQKGST
                     TTRFGTKDEFLRMVAVMHANGIEVIQDIILNHVDGAGGTDGAGGEDPSGWDNKWKNFR
                     YVSYATPAKSGNEADYWSRSGRWAKNWKNFHPNNAHNCNSGDICSDWWGPDICYWEGA
                     YGKSSNVVGYNPDQTANYMRNEARNWMMWFKKQTAVDGYRWDAVKHIPDWFQQDMLWN
                     VKYSLPSWSQGGEDMFSVGEYVGSQTEMDNYCSAVQYANGANEFTMGTFDFSLRQGIQ
                     EVIQGGGFGDIGS"
     gene            complement(1104..2924)
                     /locus_tag="PKN90_00510"
     CDS             complement(1104..2924)
                     /locus_tag="PKN90_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HNZ84798.1"
                     /translation="MQTIELLAPAKNKESGIEAILHGADAVYIGAPKFGARAAAGNSL
                     EDIAQLIEFAHIYDAKVYIALNTLIKDDELQEITDLIHSLYAINADALIIQDMGILEL
                     DIPPIALHASTQTDNRTLAKVAFLESVGFDQIVLARELSLQQIKHIAQNSSSKIEVFV
                     HGSLCVSYSGQCYISEALHGRSANRGECAQLCRLPYDLLDANKKIIAKDKHLLSLKDL
                     NLSDHLEELIDAGVYSFKIEGRLKDTAYVKNITAYYRQKIDAIIAKNNNLKRSSSGIS
                     TYTFEPKPEKTFQRSTTSYFFHKPTESVFSVLTPKSTGEYIGTIIDIQKDFFVVDTTI
                     SIANGDGFCYISTKQLLEGFRVNKAIGNRIYPSEMLQMPIGTKIYRNADFAFSKLLHA
                     KSANRKIAISISIQETNEGYLIEFCDENMNKACREIHIDNNKAQKDQQPNIEQQLSKL
                     GNTPFYCKHISIIFTDNKFIPSSVLTQIKQTLSQELLLHRQVRYKPTRIVKTNSASLF
                     PEKELSYTGNVSNNLAHKFYTKHGVIKIDKAFELEKRLPNPVLMFCKACIKDSIGFCP
                     KKNATPFPYKEPLFLKYKEQEFLLSFNCSTCEMTIVQEKI"
     gene            complement(2927..3844)
                     /gene="metA"
                     /locus_tag="PKN90_00515"
     CDS             complement(2927..3844)
                     /gene="metA"
                     /locus_tag="PKN90_00515"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNZ84799.1"
                     /translation="MPLNIPKKLPAIETLKGENIFVMDDERASTQEIRPLKIVILNLM
                     PIKITTETDLIRLLSNSPLQIEIDFLQIKSHTHKNTPIEHLMAFYKTFDAIKKNKYDG
                     MIITGAPVELMPFEEVNYWNEITEILDWAKTHVTSTLFICWAAQAGLYHYYGIPKYEK
                     PSKVFGVFEHTALDKLNPIFRGFDDSFFVPHSRHTEVKAEDILKHPALTLLSVSKEAG
                     VYMVMARNGREFFVTGHSEYSPNTLDDEYKRDIEKGLPIDLPQNYYIDNNPNNPVNVR
                     WRSHGNLLFTNWLNYFVYQETPYDIDKIK"
     gene            complement(3871..5157)
                     /locus_tag="PKN90_00520"
     CDS             complement(3871..5157)
                     /locus_tag="PKN90_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNZ84800.1"
                     /translation="MKQDTKIGTICVQGGWSPKKGESRVLPIIQSTTFKYETSEQMGK
                     LFDLEESGYFYTRLQNPTNDAVAAKIAALEGGVAAMLTSSGQAALFYAISNICSAGDH
                     FVCTSSIYGGTYNLFAVTLKRMGIEVTFVDLDADEQEIAKAFKPNTKALFAETIANPA
                     HAILDIEKFARIAHTHGVPLIIDNTFATPINCRPFEWGADIIIHSTTKYMDGHATAVG
                     GVIVDSGNFDWDAYADKFPGLTQPDDSYHGLTYTKAFGKGAYITKAVVQLMRDLGSCQ
                     SPQNAFLVNIGLETLHLRMARHCENAQKVAEFLENHSKVAWVNYSGLPSNKYYQLAKK
                     YLPKGSCGVLSFGLKAGREASIAFMDNLQLVAIVTHVADARSCVLHPASHTHRQLSDE
                     QLKQAGVAPDLIRLSVGIEDVEDIIADIEQALQKVN"
     gene            complement(5178..>6258)
                     /gene="thrC"
                     /locus_tag="PKN90_00525"
     CDS             complement(5178..>6258)
                     /gene="thrC"
                     /locus_tag="PKN90_00525"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNZ84801.1"
                     /translation="GIHVYVLYPKGLVSPIQECQFTTLGQNITAIEVDGTFDDCQALV
                     KNAFMDKELNEALKLTSANSINVARFLPQAFYYFYAYAQLKKIGKEQNMVVCVPSGNF
                     GNITAGLFAKKMGLPIKRFIAANNRNDIFLQYLQTGVYTPKPSVATIANAMDVGDPSN
                     FARILDLYKNSHEAICADISGSSYTDEEIADTLKKCYQQNDYLLDPHGACAYRALTEQ
                     IKENEIGVFLETAHPAKFLETVQNIIKQEVVIPEKLQEFMKGKKEVEPMSKEFSAFKA
                     FLMVDGIQNNNNKKTIVLKSYDNPAIANLEHGILQDAGVECFLSDESVVNLYPMFSSP
                     FGGVKLHVFEEDKEKALEILGTTNS"
BASE COUNT         1820 a         1162 c         1043 g         2233 t
ORIGIN      
        1 ttgatccaat atcaccaaaa cctcctcctt gaataacttc ttgaatacct tgtctgagag
       61 aaaaatcaaa tgttcccata gtaaattcat tggcaccatt tgcgtattga accgcactac
      121 aataattatc catttcggtt tgactaccaa catattcgcc tacactaaac atatcttcgc
      181 ctccttgact ccaactgggc aaggagtatt tcacattcca aagcatgtct tgttgaaacc
      241 aatcaggtat gtgttttact gcatcccacc gataaccatc aactgctgtt tgttttttaa
      301 accacatcat ccaattacgt gcttcgtttc gcatgtagtt tgctgtttgg tctggattgt
      361 aacccactac gttagaactt tttccgtatg ctccttccca gtaacaaata tctggtcccc
      421 accaatcaga acagatatct ccactattac aattgtgggc attgtttgga tggaagtttt
      481 tccaattttt tgcccatctt ccacttctac tccaataatc tgcctcattg ccacttttgg
      541 ctggagtagc atacgataca tacctaaaat ttttccattt attatcccag ccagaggggt
      601 cttctcctcc tgcaccgtca gtacctcctg caccatcaac gtggtttaaa ataatgtctt
      661 ggattacttc aattccattg gcatgcatta ccgcaaccat acgtaaaaac tcgtctttag
      721 ttccaaaacg tgtggtagta gaaccttttt gatacttatc tcccaaatca taatgatcaa
      781 aaggggaata tccaacagaa tttgttccag tatttttata agaaggaggt atccatacag
      841 cattaattcc atattctttt agcctaggag ctaactcagt aaggtagttt gcataactat
      901 ttgggtagtt ttcattccaa tagttccacc aaaatgcttg caatacgact cttttatgaa
      961 catcagcttg tagttgcatt ggaacgagaa atccactaat tactagcatc aaggcaaata
     1021 atttactgta caattttctc ataagtagaa ggtattaagt aacttacaaa tatagaatga
     1081 aaattgtatt attccaaaaa aaattatatt ttttcttgaa caatagtcat ttcacatgtt
     1141 gagcagttaa atgataacag aaactcttgc tctttatatt ttaaaaataa aggttcttta
     1201 taagggaagg gagttgcgtt ttttttggga cagaatccta ttgagtcttt gatacatgct
     1261 ttacaaaaca taagtacggg gttgggtagc cttttttcaa gttcaaaggc tttgtctatt
     1321 tttataaccc catgtttggt ataaaattta tgtgctaggt tattgcttac gttgcctgta
     1381 taacttaatt ctttttcagg gaataaggat gccgagtttg tttttactat ccttgttggc
     1441 ttatatctta cttgtctatg taaaagtaat tcttgtgaaa gagtttgttt tatctgtgtt
     1501 aatacagaag aaggaataaa tttattatcc gtaaatataa tggagatatg tttgcaataa
     1561 aatggtgtat ttcctaattt tgaaagttgt tgttcaatat ttggttgctg atctttttgt
     1621 gctttgttgt tatcaatatg tatttcccta catgctttat tcatattttc atcacaaaac
     1681 tcaattaaat acccctcatt tgtttcttgt attgagatag aaatagctat ttttctattt
     1741 gctgattttg catggagtaa ttttgaaaaa gcaaaatcag catttctata tattttggtt
     1801 ccaataggca tctgtagcat ttcagatgga taaatgcggt ttccaatagc tttgtttact
     1861 ctgaatcctt ccagtagttg ttttgtagat atatagcaaa atccatcacc atttgctatg
     1921 gatatagtgg tatcaactac aaaaaaatct ttctgtatat caattatggt accaatatat
     1981 tccccagttg attttggagt taaaactgaa aatactgatt cagtaggttt gtgaaaaaaa
     2041 taggatgtag tacttcgttg gaaggttttc tctggtttag gttcaaatgt gtaggttgat
     2101 atgccagatg atgagcgttt tagattatta ttttttgcaa taattgcatc tattttttgt
     2161 ctgtagtaag ccgtaatatt tttaacatat gcagtatctt ttaatcgtcc ctctatttta
     2221 aacgaataaa ctccagcgtc aattagttct tcaaggtgat cagacaaatt taaatcttta
     2281 agcgataaaa gatgcttatc ttttgcaatt atttttttat tagcatctaa taagtcgtat
     2341 ggaagcctac ataattgagc acactcacct cgatttgcac ttcgcccatg caaagcttcg
     2401 cttatatagc attgtccgct gtagcttaca cataatgagc catgcacaaa tacttctatt
     2461 ttactagagc tattttgagc aatatgtttg atttgttgta acgataattc tcgtgccaat
     2521 actatttggt cgaatcctac agactctaaa aatgcaactt tagctaatgt cctattatct
     2581 gtttgtgtac tagcatgcaa cgcaataggc ggtatgtcaa gttccaaaat gcccatatct
     2641 tgtatgataa gtgcatctgc attaattgca tataaagagt gaattagatc tgtaatttct
     2701 tgcagctcat catccttaat taatgtattt agtgcaatat atacttttgc atcatatata
     2761 tgagcaaatt cgataagttg agcaatatct tctagcgaat ttccagcagc agcccttgct
     2821 ccaaattttg gagctccaat ataaaccgca tctgcaccat gtaatatggc ttcaatccca
     2881 ctttccttgt tcttagcagg ggcaagaagt tcgatggttt gcatgattac ttaattttgt
     2941 ctatatcgta aggtgtttct tggtacacaa aataatttaa ccaattagta aacagtaaat
     3001 taccatggct tctccaacga acattaacag gattgtttgg attattgtca atataataat
     3061 tttgcggtaa atcaataggt aatcctttct ctatatctcg tttatattca tcatctaatg
     3121 tattaggaga atactcagaa tgtcctgtta caaaaaattc tctgccgtta cgagccatta
     3181 ccatatatac tccagcttct ttcgatacgg ataataaggt taaggcggga tgtttgagaa
     3241 tatcttctgc ttttacttcg gtgtgtcggc tatgaggcac aaagaagctg tcgtcaaatc
     3301 cgcgaaatat aggattaagt ttgtctaatg ctgtatgttc aaacacacca aatactttgc
     3361 ttggtttttc atatttaggt atgccataat aatgatacaa accagcttgt gccgcccaac
     3421 aaataaagag ggtagaagta acgtgcgttt tagcccaatc gagtatttca gttatttcat
     3481 tccaataatt tacttcttca aaaggcatta attctactgg agccccagtg ataatcattc
     3541 catcgtattt gttctttttt attgcatcaa acgtcttata aaaagccatt aaatgctcta
     3601 taggcgtatt tttgtgtgta tgacttttta tttgcaaaaa atcaatttct atttgcaatg
     3661 gcgagtttga caataaccga attaaatccg tttcggttgt tatcttaata ggcattaagt
     3721 taagaatcac tattttaagt ggtctaattt cttgggttga tgcacgttca tcgtccatta
     3781 caaaaatgtt ttctcctttt aatgtttcta ttgcggggag ttttttagga atatttaatg
     3841 gcatgatgtg tttgtttttt tagtgataca ttaatttact ttttgcagtg cttgttcaat
     3901 atcagcaatg atatcttcta catcttctat ccctactgaa agacgaatta agtcaggcgc
     3961 tacacctgct tgtttaagtt gttcatcaga caattgacgg tgtgtatggc ttgctggatg
     4021 tagcacgcac gaacgagcat ctgccacgtg tgtaacaata gctactaatt gcaagttatc
     4081 cataaatgcg atagatgcct ctcttccagc ctttaatcca aaagataata caccacacga
     4141 accttttggt aaatattttt tagctaactg gtagtattta ttcgagggta atccactata
     4201 atttacccaa gcgactttgc tatgattttc taaaaattct gctacttttt gtgcattttc
     4261 gcaatgtcga gccatgcgta gatgcaaggt ttctaatcca atgttcacta aaaaagcatt
     4321 ttggggcgat tgacatgaac ctaaatcacg cattaattgt accacagctt ttgtaatata
     4381 agctcctttg ccaaaagctt tggtgtatgt taatccatga tatgaatcat caggttgtgt
     4441 taatcccggg aatttatcag catatgcatc ccaatcaaaa ttgccactat ctacgatgac
     4501 accacctact gcggtggcgt gtccatccat atatttggta gtggaatgga taataatatc
     4561 tgctccccat tcgaatgggc gacaattgat aggtgttgca aatgtattgt caataattaa
     4621 tggcacacca tgtgtatgtg caattctagc aaacttttct atatctaaaa ttgcatgagc
     4681 tggattagcg attgtttcag caaataaagc ttttgtattt ggtttaaatg cttttgctat
     4741 ttcttgttca tctgcatcta aatcaacaaa tgttacttca atacccatac gcttgagtgt
     4801 aacagcaaac aaattgtatg tgcctccata aatgctagaa gtacatacaa aatgatctcc
     4861 agccgaacat atatttgata ttgcgtaaaa taatgcagct tgacccgaag aggttagcat
     4921 agcagctacg ccaccttcta aggcagctat ttttgcagct actgcatcgt tggttgggtt
     4981 ttgtaaacga gtataaaaat agccactttc ttccaaatca aagagcttgc ccatttgttc
     5041 gctcgtttcg tatttaaagg tagtactttg tatgataggt agtacacgcg attctccttt
     5101 tttaggactc catccgccct gtacacatat agtcccaatt tttgtatctt gtttcattgt
     5161 tattcttttg ttaataatta cgaattagtt gttcctagta tttccaatgc tttttcttta
     5221 tcttcttcaa aaacatgcag tttaactcca ccaaaaggag aagaaaacat tggatacaga
     5281 tttaccacac tttcgtcgct caagaaacac tcaactcctg catcttgcag tatgccatgt
     5341 tctaaatttg caatagctgg gttatcgtac gatttaagaa cgattgtttt tttattgtta
     5401 ttgttttgta ttccatcgac catcaaaaaa gctttgaaag cagaaaattc tttgctcatt
     5461 ggttcaacct ctttttttcc tttcataaac tcttggagtt tttcaggaat tacaacttct
     5521 tgtttaatta tattctgaac ggtttcaaga aatttagctg gatgagctgt ttccaagaaa
     5581 actccaattt cattttcttt tatctgttct gtaagagcac ggtatgcaca agctccatga
     5641 ggatcaagca aataatcgtt ttgctgatag cattttttta acgtatcggc tatttcttca
     5701 tctgtataac ttgatccact aatgtcagca caaatagctt cgtgtgagtt cttatacaag
     5761 tcaaggatac gtgcaaagtt acttgggtcg cctacatcca ttgcattggc tatggtggct
     5821 accgatggtt ttggggtgta cacacccgtt tgtaaatact gtaagaatat atcgttacga
     5881 ttgttcgctg caataaaacg cttgataggc aatcccattt tttttgcaaa caaacctgct
     5941 gtaatattac caaaattacc gcttggtaca catacaacca tgttttgttc tttcccaatc
     6001 tttttcaact gagcatatgc ataaaagtaa taaaatgcct gtggcaaaaa gcgtgctacg
     6061 tttatcgagt ttgctgatgt aagttttaat gcttcattga gttctttgtc cataaaagca
     6121 ttcttaacca atgcttggca gtcgtcaaat gttccgtcta cttctatggc tgtaatattt
     6181 tgtcctaatg tagtaaactg acattcttga atagggctaa ccaatccttt aggatacagc
     6241 acatatacat gaatacct
//
LOCUS       DAOPUR010000017         6247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_12456_length_6247_cov_3.218185, whole genome shotgun sequence.
ACCESSION   DAOPUR010000017 DAOPUR010000000
VERSION     DAOPUR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12456_length_6247_cov_3.218185"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1990)
                     /locus_tag="PKN90_00530"
     CDS             complement(<1..1990)
                     /locus_tag="PKN90_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNZ84802.1"
                     /translation="MNATIISLIAKQLGISEKQVKNTADLLGSGNTIPFISRYRKETT
                     GNLDEVQIGNINELVQYFEELNKRKETILNTIEEQGSLTPELAEKIATCWESNALEDM
                     YLPYKPKRKTKGQTAKENGLEPLAKILMSQSADDPYRIAQRFVTGNVQNNEAAVQGAL
                     DIIAEWINESAIARNRIRQIFAYDAVITANIIKGKEQEGAHYENYFKVKEPLKKCSSH
                     RLLAIRRGETEGFLRVSISPSDEEVCLTKLTQIFVKNNTPSAQLVATACKDAYKRLLK
                     PSIETEFAHSSKELADKQAIAVFANNLKQLLLGSPLGEKRVLAIDPGYRTGCKVVCLD
                     AQGNLLHNETIYPHPPQKETAQAARKIQQLVETYAIQAIAIGNGTASRETETFMQHIR
                     YPLAVDIFVVSENGASVYSASKIARDEFPQYDVTVRGAISIGRRLMDPLAELVKIDPK
                     SIGVGQYQHDVDQNKLQTALDETVVHAVNLVGVNLNTASEHLLRYVSGLGPNLAKNIV
                     AYRSQNGEFTSREELKKVPRMGEKTFEQAAGFLRIAGAKNPLDNSAVHPESYYVVEKM
                     AKDLNCQLADLIGQKELAQRIQLERYIDQHIGLLSLNDILKELEKPGRDPRKKVSVIT
                     FDQRITTINDVQVGMLLDGIVTNITNFGVFVDIGIKENG"
     gene            complement(1991..2680)
                     /locus_tag="PKN90_00535"
     CDS             complement(1991..2680)
                     /locus_tag="PKN90_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNZ84803.1"
                     /translation="MIGTLVNTAAVIVGGSLGMFIKHRLPEKYVRIYFQAVGLFTIVL
                     GISMSLKMAFPLMVVASLACGGILGEWLQLEEKTNRLGETLKKKLRFSNERFTEGLVT
                     AFLLFCMGSMSIIGAIEEGFGKTSDLLLTKSLMDGFSALMLASALGVGVVFSSIPLLL
                     FQGGITLMVWMFAKDIPTIYITEITAVGGILLIGLGLSILEIRKLNIINLLPSLVFIC
                     LFLYIRSYFAI"
     gene            2960..3172
                     /locus_tag="PKN90_00540"
     CDS             2960..3172
                     /locus_tag="PKN90_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HNZ84804.1"
                     /translation="MVVSNNFYNFKNMKSFDLNSYGVQEISVQEMKEVGGGIGVFAVA
                     IVGLAVLYVAGTVIGLYNGHKFGGMP"
     gene            3265..3636
                     /locus_tag="PKN90_00545"
     CDS             3265..3636
                     /locus_tag="PKN90_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84805.1"
                     /translation="MLTSFIQKLSELLTKKNISEVYDWEINVFYSEAFDMNRDLVLSL
                     DIESLLDICKHNQVYCNEKGRILAELLYFETLRDKKSTFVKNYAKKAIQLFHYYTQYT
                     KTYDLDVINKITQLSKIIENE"
     gene            4253..6211
                     /locus_tag="PKN90_00550"
     CDS             4253..6211
                     /locus_tag="PKN90_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HNZ84806.1"
                     /translation="MHIIQSYWNPNTKEDVLLQTGGFVCPEIHWMAWAFSCLQLRKFY
                     PNVELHTNKAGKELFELLGLPYTAIHTSMETDFMRNLHPSMWAYAKIHTYSLQTESFL
                     HVDGDVFIWKAFDEQLMSSPLIAQNIEDNLIFYNHSIEVIEKHADVVPEWVGKYKHHP
                     KAYNAGILGGNDIYFFKQYTQLALEYYQNNENKLNKMINEDSNVNTIPEQYLFYVLSQ
                     DSNVPVAVFSNKNEGVCNPDDFLRFVEISKIPYQEQYCHILGKLKTHNHLSNFVNFVL
                     QNDYPQYWEHIIHVFKERGILSEYMQRLLTMQEKDKKTILPQIMPSKGTYRDTRTLCK
                     LFDVAFDPQDLSQNFENPIIQDLYEYERKIHEFNNRTFVANHHSVPFPTYNAEGNVFN
                     QKDYLDYYVYVTPYHQFVNTRYNWHKLFELKTAQEIVAHAYPYKLTTLLSLDGYYTLN
                     DNFVHDALVTLIETIKLKPITIKELLKNDVAEKEKTLEMLTSWYSAGFIYLSKSKKDF
                     IPQPPSEEYTYSQANLKNQVSTCLGYVLTHYGITNHDRKLLQKYQDIQRNVTMHEVVQ
                     TLEKIGLQAKGVRGNMDSLTKIPLPAICIVTLREYLPLYVVIIKSTATHITIYNTEMM
                     QEENYPVAYFSTIWDGNLILVLPQGANN"
BASE COUNT         1988 a         1141 c         1094 g         2024 t
ORIGIN      
        1 agccattttc cttgataccg atatctacaa acacaccaaa gttggtgata ttggttacaa
       61 tcccgtcaag taacatgcct acttgcacat cgttgatagt agtaatgcgt tggtcgaaag
      121 tgattacgct gactttttta cggggatctc tacccggttt ctcgagttct tttaagatgt
      181 cgttgagcga aagtaaacct atgtgttggt cgatataccg ttcgagctgt atgcgttgtg
      241 ctagctcttt ttgaccaatt aaatcagcca attgacaatt caggtctttt gccatttttt
      301 ctactacata gtaactttcg gggtgcacag cagagttatc caagggattt ttggctccag
      361 caatgcgtaa aaatcctgcg gcttgctcaa aggttttttc acccatgcgt ggcacttttt
      421 ttagctcttc cctgctggtg aattcgccat tttgcgaacg atacgctaca atatttttag
      481 ccaaatttgg tcccagtccc gacacataac gcaacaaatg ttcgctggca gtatttaaat
      541 tgacacccac taaatttacc gcatgtacta ccgtttcgtc taatgctgtt tgcaatttat
      601 tttgatccac atcgtgctgg tattgcccta cgccaatgga ttttgggtct atttttacca
      661 attcagccaa gggatccatc aacctacgtc caatggatat agctccacgc accgtaacat
      721 cgtattgagg aaactcatcg cgggcaattt tagaagccga atataccgaa gccccatttt
      781 cgcttaccac aaaaatatct actgccaagg gatagcgaat gtgttgcata aacgtttccg
      841 tttctctgga ggcagttccg ttgccaatag caatggcttg tatggcgtat gtttctacca
      901 attgttgtat tttgcgggca gcctgtgcgg tctctttttg tggtggatgc ggatagatgg
      961 tttcgttgtg tagcaaattg ccttgagcat ccaaacatac cactttacac cctgttcggt
     1021 agccagggtc gatggcaagc accctttttt cgcccaaagg agatcctaat aacaattgtt
     1081 tcaggttatt ggcgaaaacg gcaatggctt gtttgtcggc aagctcttta ctgctatggg
     1141 caaattctgt ttcaatagac ggttttagca aacgtttata cgcatctttg caggcagtag
     1201 ctacgagttg agccgatggc gtgttgtttt ttacaaaaat ctgtgtcagt tttgtaaggc
     1261 atacctcttc gtccgatgga ctgatggata cccgtaaaaa tccttcagtt tctcctctac
     1321 gaatggctaa taagcggtgc gagctacatt tttttagtgg ctcttttacc ttaaaataat
     1381 tttcgtaatg agccccttct tgctcttttc ctttgataat attggctgta atcacagcat
     1441 cgtatgcaaa tatttggcga atgcggttac gtgctatggc actttcgtta atccactcgg
     1501 caatgatatc caaggctcct tgcacagccg cttcattgtt ttgtacattg cctgtaacaa
     1561 atcgctgagc aatgcgataa gggtcgtctg ccgattgact catcagtatt tttgccaagg
     1621 gttctaagcc attttctttg gcggtttgcc ctttggtttt acgttttggc ttgtagggca
     1681 aatacatatc ttctagtgcg ttgctttccc aacaagtggc tattttttca gcaagttctg
     1741 gggtcaaact cccctgttct tcaatggtgt tgaggatggt ttctttgcgt ttatttagtt
     1801 cctcaaaata ctgtactaat tcattgatat tgcctatttg cacttcatct aagttgcctg
     1861 tagtttcttt gcggtagcga ctgataaatg gaatggtatt gcccgaacca agcaagtcgg
     1921 ctgtgttttt tacttgtttt tcgctaatgc caagttgttt ggctatcaat gatatgatgg
     1981 ttgcattcat ttagatggca aaatatgaac ggatatataa aaatagacaa ataaagacaa
     2041 gcgatggcag taagttgatg atgttgagtt ttcttatttc taaaatggaa agacctagtc
     2101 caatcaacaa aataccccca acagccgtta tttcggtgat gtaaattgta ggaatgtctt
     2161 ttgcaaacat ccataccatc agcgtaatac ctccctgaaa tagcagtagc ggtatagatg
     2221 aaaataccac accaacaccc aatgccgaag ccaacatcaa ggcagaaaaa ccatccatca
     2281 aggatttggt taataacaaa tcggatgttt taccaaaacc ttcttcaata gctccaataa
     2341 ttgacatgga acccatacaa aaaagcaaaa aagccgtaac caagccttcg gtaaaacgct
     2401 cgttggaaaa acgcagtttc ttttttaaag tttcgcccag tcggtttgtt ttttcttcga
     2461 gctgaagcca ttcgccgagt attccgccac atgccaaact ggctactacc atcaagggaa
     2521 atgccatttt tagcgacatg ctaatgccca atactatggt aaacaaacca accgcttgaa
     2581 aatagatacg cacgtatttt tcgggcaaac ggtgtttgat aaacataccc aaactgcctc
     2641 ccactattac cgcagctgta tttactagtg ttccaatcat cttattttaa ttaattcgac
     2701 acaaaaataa caaaataatt gctttgtgtc tgaaaaatac ggatatttgt tggtaaatat
     2761 gcttgatatg ataattttta cataagctaa atttattaca tgagaacttt tagagcttta
     2821 atattagtta aaacaataca ataaatgcaa aaaaaacaga tatgcctcaa ttttctttat
     2881 ctttgtgccc atgcttgttg gagcatagat acgaaggagt taaagccacg gactctttct
     2941 attgaaaata ataaaagacg tggttgtttc gaataatttt tataatttta aaaacatgaa
     3001 aagttttgat ttgaacagtt atggcgtaca agaaattagc gtgcaagaga tgaaagaagt
     3061 tggcggtggt attggggtat ttgcagtagc aatagtcggt ttagccgtac tttacgttgc
     3121 aggcacagta ataggtttat ataatggaca taagtttggt ggaatgccgt aatttaaaat
     3181 tcacatgata aaagttgtga atagcagtaa tacctatttt gaaaatattt aataatgcta
     3241 aatgaaaaag atgtcttaat aagaatgcta actagcttta ttcaaaagct atcagaatta
     3301 ctaacaaaga aaaatatttc ggaggtttac gattgggaaa ttaatgtttt ttattcggag
     3361 gcatttgata tgaatcgtga tttagtgtta tctttagata tagagtcatt gttagacatt
     3421 tgtaaacaca atcaagtata ttgcaatgaa aaaggtagga ttttagctga acttctttat
     3481 ttcgaaacac tccgagacaa gaaaagtact tttgttaaaa attatgcaaa aaaagccata
     3541 caactatttc attattatac tcagtatact aaaacatatg accttgatgt cattaacaaa
     3601 atcactcaac tttcgaaaat aattgaaaat gaataaatat tttttgtttt ttttgtttac
     3661 catcactaga ttttttgacc tgataactac ttacctcgca acaaatgatc tatcttatga
     3721 aacaagtcct ttaattattt atttaaatat ggattggttt ggtctaatat tatccaacat
     3781 tatttttgtt atcataatat tcatattgtt agtgtatatt aaacaattta atattattta
     3841 tctagaagaa aagtatgaaa ataagatact aagcttgact gattactaca agtatattat
     3901 cttaaaatta aaaagacctg aaactaatat gagtttgttt caacttttaa aagcaaaaat
     3961 aaacgtatct gcatttctgt tttttgctac acgtggatta actcttactt ttattttgat
     4021 aagtacagtt gtatcactaa ataatatctt acaacataat aaattgtatc tgcttgaagt
     4081 tcatccattt gtaattttca atatactatt tttaatcatt atagttttat ttattctatc
     4141 ttttcacttt tttgtaaaac agagatttaa aaatatacaa taaggttttg gattttctaa
     4201 tgattttttt ttaacaattt tagtcacgta ttaaaaccga caatatttta acatgcatat
     4261 tatccaatca tactggaacc ctaatacaaa ggaagatgta ctattgcaaa caggtggttt
     4321 tgtatgcccc gaaatacatt ggatggcatg ggcattttcg tgtttgcaat tgcgtaagtt
     4381 ttacccgaat gtagaactac ataccaacaa ggcagggaag gaattgttcg aattgcttgg
     4441 cttaccctat accgccatac ataccagtat ggaaaccgac tttatgcgta acctccaccc
     4501 cagtatgtgg gcttatgcca aaatacatac atacagctta caaaccgaat cctttctgca
     4561 tgtggatggc gatgtattta tatggaaagc atttgacgaa caattaatga gtagccctct
     4621 tatagcccaa aacatagaag ataatcttat tttttataat catagtattg aagtgattga
     4681 aaaacatgct gacgttgtcc ccgaatgggt aggaaaatac aaacatcacc ccaaagcata
     4741 taatgcagga atactgggcg gtaacgatat ctattttttt aaacaatata cccaattggc
     4801 actcgaatat tatcaaaata atgagaacaa actaaacaaa atgattaacg aggatagtaa
     4861 tgtaaacact atccccgaac aatacttatt ttatgtatta agtcaagata gtaacgtccc
     4921 tgttgctgta ttttcaaaca aaaatgaagg tgtgtgtaat cctgatgatt ttttgcgatt
     4981 tgttgaaata agtaaaattc cttatcagga acaatattgt catattcttg gaaaattaaa
     5041 aactcataat catttatcaa attttgtaaa ctttgttttg caaaatgatt accctcaata
     5101 ttgggagcat attattcacg tttttaagga aagaggaata ttgtcagaat atatgcaacg
     5161 tctattgaca atgcaagaaa aggacaagaa aaccatttta ccacaaatca tgccttcaaa
     5221 aggaacttat cgcgataccc gtactttatg caaactattt gatgtagcat ttgaccctca
     5281 agatttatcc cagaattttg aaaatccaat aatacaagac ctttacgaat atgagagaaa
     5341 aatacatgaa ttcaacaacc gtacgtttgt agcaaatcat cattctgtac catttcctac
     5401 ctataatgca gaagggaatg tattcaacca aaaggattat ttggattatt acgtgtacgt
     5461 aacaccatat catcaatttg ttaatacacg atacaattgg cacaaactgt ttgaattaaa
     5521 aactgcacaa gagatagtcg ctcatgctta cccgtataag ttaacaactc tcttatcatt
     5581 agatggctat tatacattaa atgataattt cgtacacgat gcattggtaa ctctaattga
     5641 aaccatcaaa cttaaaccta ttaccataaa ggagttactc aaaaatgatg ttgcagaaaa
     5701 agagaaaacc cttgaaatgc ttacctcgtg gtattcagca ggttttattt acctttccaa
     5761 atctaaaaag gattttattc cacagccacc atcagaagaa tatacctact cgcaagccaa
     5821 cctgaaaaat caggtaagca cctgcttagg ttacgtgctt acacattacg gaataacaaa
     5881 tcatgataga aaattattac aaaaatacca agacattcag cgtaatgtta ccatgcacga
     5941 ggtagtacag actctcgaaa aaattgggtt acaggcaaaa ggggttcgtg gcaatatgga
     6001 tagtttaacc aaaataccac tccccgctat ttgtatagtt acattacgcg agtacttgcc
     6061 actgtatgtg gtaataatca aatccactgc cacacacata actatataca atactgagat
     6121 gatgcaagag gaaaactacc ctgttgcgta tttttctact atttgggatg gcaatttgat
     6181 tttggtgttg ccacagggag ctaataatta aatattttcg gtttattggt agagatgcaa
     6241 acaagat
//
LOCUS       DAOPUR010000018         6153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_12698_length_6153_cov_3.495244, whole genome shotgun sequence.
ACCESSION   DAOPUR010000018 DAOPUR010000000
VERSION     DAOPUR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12698_length_6153_cov_3.495244"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKN90_00555"
     CDS             complement(<1..484)
                     /locus_tag="PKN90_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ84807.1"
                     /translation="MKQLISFIQKEFRHILRDRTTVMILLLMPIIQIILFGFALTTEV
                     KNTEVAVLVPTNDEVTDRIVDKIHASAYFDVVQNIYGTNEIETVFRDGKAKLVVVFEN
                     HFAQNLQHTGKAHIQLIADATDPNAANSFVFYAGNIIAAYQQELSSQGKEGLLIQPEV
                     K"
     gene            complement(495..1259)
                     /locus_tag="PKN90_00560"
     CDS             complement(495..1259)
                     /locus_tag="PKN90_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ84808.1"
                     /translation="MNRKILHNADEVIVVENLTKKFGNFTAVDAISFKVKRGEIFGFL
                     GANGAGKTTAMRILCGLSIPSGGKGMVAGYDIAHEQEKIKKNIGYMSQKFSLYDDLKV
                     WENIELFGGIYGMKDIDIKRKTNELMLQLQFENERDKLVKSLPLGVKQKLAFSVAIFH
                     NPQVVFLDEPTGGVDPITRRQFWELIYDATAKGITVFVTTHYMDEAEYCDRVSIMVDG
                     KIEALDSPKNLKNQFQATNMNEVFQQLARKATRADS"
     gene            complement(1266..2183)
                     /locus_tag="PKN90_00565"
     CDS             complement(1266..2183)
                     /locus_tag="PKN90_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ84809.1"
                     /translation="MNTDTAIQCQSISKKYDKIEALKDISFETKKGEIFGIIGPDGAG
                     KTTLFRILTTLILADNGTATVDGFDVVTDYKEIRKRVGYMPGRFSLYQDLTVEENLNF
                     FATVFNTTVEANYALIKDIYQQIEPFKTRRAGKLSGGMKQKLALCCALIHKPSVLFLD
                     EPTTGVDAVSRKEFWDMLQSLKTQGITILVSTPYMDEASLCDRIALIKDGEFLKIDTP
                     KNIVKQFEKTLWIVQGDDMPKLLKDLRAMQQIKSCYAFGDSHHVTVEKGELTMDILKE
                     YLVKNGHSNVCIQSIVPTVEDCFMELSNR"
     gene            complement(2205..3140)
                     /locus_tag="PKN90_00570"
     CDS             complement(2205..3140)
                     /locus_tag="PKN90_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNZ84810.1"
                     /translation="MKTKNKKQAIFLRNSCRIGLLATIVSIFLVSCSNSDGDVDASGT
                     FETTEIIVSTESMGKIMQLNVEEGQQLNFNQRVGYIDTTQLYLKKLQLVASKKALQSR
                     RPDIQKQIAALEQQIETAKTERKRVENLVKAEASTTKQLDDANAQIKVLEKQLEATKS
                     SLENTSNSILGDNEALQIQIEQIEDQLQKCYITSPISGVVLTKYAEQGELATPGKALF
                     KIGDVTNMILRAYVTSDQLTQIALGQKVKVYADYGEDRKEYEGVVSWISSKSEFTPKT
                     IQTRDERANLVYAVKINIKNDGLLKIGMYGNVKFN"
     gene            complement(3150..4421)
                     /locus_tag="PKN90_00575"
     CDS             complement(3150..4421)
                     /locus_tag="PKN90_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ84811.1"
                     /translation="MKKIWLCSVIAMAFQLISAQSVTLEECWEKARINYPLIKQYGLI
                     ENTTAYNVSNATKAYLPKLLLSAKATYQSDVTELPEALSQRLGISMEGLSKDQYQLVG
                     EISQLMWDGGATHAQKEVVKTLSKVEKQKVEVDLYALKERVSQLYFGILLIDEQVHLN
                     TMLKDELQTHYKRIEAYKQNGVANQSDLDAIQVEQLNVEQRNTELVNTKLAYVRMLSI
                     FTKMELDEKVTLSKPEKNLLILGDTTNNRPELTLLAYQSEYYESQKKIIHANNLPKLS
                     AFFQGGYGKPALNMFANEFAPFYIGGIRFSWNLGELYSTKNNTSKLDINKQSIDVQKE
                     TFLFNTDLKNEQDKLAIKNLQALLNTDDDIIRLRENIKKVAVSKVENGTLTVADLIRE
                     INAENQAKQLKSLHEIQLIKRLYEVKNNINK"
     gene            complement(4452..5075)
                     /locus_tag="PKN90_00580"
     CDS             complement(4452..5075)
                     /locus_tag="PKN90_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ84812.1"
                     /translation="MSINNVNSMEEIILETAERLFLENGYASTSTTLIAKQVGCNQAL
                     VHYYFRTKEKLFNTIFEKKFKAFFQQIFDMNHLENLSFLDKLKKIIETHFDLISQNPQ
                     IPQLIISELPRLPEQIKSLKEKLYSYPESLFAQLGEELQVEIAAGRVRNVDLTDVIIT
                     IVSLNAALFTFLPIVASIVELDENERKSILAHRKIEHVNIVMSYLRP"
     gene            complement(5199..>6153)
                     /gene="arsB"
                     /locus_tag="PKN90_00585"
     CDS             complement(5199..>6153)
                     /gene="arsB"
                     /locus_tag="PKN90_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNZ84813.1"
                     /translation="GKLLPIVPETLGLLEYANVSIPIAVLIWIMIFPIMLKIDFKSVV
                     DAVKMPKGLTITLVVNWLIKPFTMFGFAWLFFMVIYSLWIPEDLAKEYLAGAVLLGAA
                     PCTAMVFVWSHLTKGNPAYTLVQVAINDLIIIVAFIPIVTLLLGVSGIVIPWNTLIMS
                     VLLFVVIPLVLAYITRNLIIRKKGIAYFTDVFIKKFSATTISGLLLTLVIIFTFQGDV
                     ILQNPLYILLIAVPLIIQTFFIFFVAYRWAKAWKLPHNIAAPASLIGASNFFELSVAV
                     AIVLFGLQSGATLVTVVGVLVEVPVMLALVKIANKTKHQFV"
BASE COUNT         1845 a         1234 c          939 g         2135 t
ORIGIN      
        1 tcttaacttc cggttgaata agtaaacctt ccttgccctg agaacttagc tcttgttggt
       61 atgcagcaat aatattgcca gcataaaaca caaacgaatt ggcagcattg gggtctgttg
      121 catcggcaat aagctgaata tgagctttgc ctgtatgctg aagattttgt gcaaaatgat
      181 tttcaaaaac cacaactagt tttgcctttc catctctgaa tacagtttct atttcatttg
      241 taccataaat attttgtaca acatcaaaat aggcactggc atgaatcttg tcaacaatcc
      301 gatccgttac ttcatcattt gttggtacca aaacagcaac ttcggtattt tttacttctg
      361 ttgtcagtgc gaatccaaat aaaattatct gaataatcgg cattaaaagt aaaatcatca
      421 ccgttgttct atcacgtaaa atatgacgaa attctttttg tataaatgat ataagttgtt
      481 tcataacgat gcttttaact gtctgctctg gtggcttttc gggctaattg ctgaaaaacc
      541 tcattcatat ttgttgcctg aaactgattt ttcaagtttt tgggcgagtc taatgcttct
      601 atttttccat ctaccataat agatacccga tcacaatact cagcttcatc catataatgt
      661 gttgttacaa aaaccgttat acccttggcg gttgcatcat aaattaattc ccaaaattgg
      721 cgacgagtta ttggatctac ccctccagtg ggttcatcca aaaaaactac ttgtggattg
      781 tgaaaaatag ccaccgaaaa tgccaatttt tgtttaaccc ccaatggcaa ggattttacc
      841 aatttatcac gttcattctc aaattgaagt tgtagcatta attcattcgt ttttcttttt
      901 atgtcaatat ctttcatgcc ataaatgcca ccaaagagct cgatattctc ccataccttt
      961 aaatcatcat aaagagaaaa cttttggctc atatagccga tatttttctt tattttttcc
     1021 tgttcatgag caatatcata gcctgctacc attccttttc ctccgctagg aatactcaat
     1081 ccacataaaa tacgcatagc cgtagttttt ccagctccat ttgctcccaa aaaaccaaaa
     1141 atttcacctc gttttacttt aaacgagata gcatctactg ctgtaaaatt gccaaatttc
     1201 tttgttaaat tttcaacaac aataacctca tctgcattat gtaagatttt tctattcatt
     1261 ctcttctacc tgtttgaaag ttccataaaa caatcttcta cggtcggcac aatggactga
     1321 atacacacat ttgaatgtcc atttttcaca agatattctt ttaaaatatc cattgtcaac
     1381 tctccttttt ctactgtaac atggtggcta tcgccaaaag cataacaact tttaatttgc
     1441 tgcatagccc gcaagtcttt tagtaacttc ggcatatcat ctccctgaac aatccaaagt
     1501 gttttttcaa attgtttgac aatgttcttg ggtgtatcaa tttttaaaaa ttcgccatcc
     1561 ttaattaatg caatacgatc acacaagctt gcttcatcca tgtatggcgt tgaaacaaga
     1621 atagtgattc cttgtgtctt caaactctga agcatatccc aaaattcctt gcgagaaact
     1681 gcgtcaactc cggtagtcgg ctcgtcaaga aaaagaacac ttggtttatg aatcaaagca
     1741 caacataatg ccagtttttg tttcattcct cccgaaagtt ttcctgccct ccgcgttttg
     1801 aagggttcta tttgttggta aatatctttt atcaacgcgt agtttgcttc aacggttgta
     1861 ttaaaaacgg ttgcaaaaaa attcaaattt tcttccaccg tcaaatcctg atacaacgaa
     1921 aatcgtcctg gcatataccc cactcgttta cggatttcct tgtaatcggt tacaacatca
     1981 aaaccatcaa ctgttgccgt tccattgtca gccaaaatca aggtagtaag aatacgaaac
     2041 agggttgttt ttccggctcc atcaggacct ataatcccga atatttcgcc ttttttagtt
     2101 tcgaaagaaa tatctttcaa agcctcaatc ttgtcgtatt ttttgcttat tgattggcat
     2161 tgaatggcag tatccgtatt catatattct ccaattcttt atctttaatt gaattttaca
     2221 ttgccataca taccaatttt caacaaccca tcgttcttta tgttgatttt tacggcatac
     2281 accaaattag cacgttcgtc gcgtgtttga atggttttgg gggtaaattc cgatttgctc
     2341 gaaatccaac taacaacgcc ttcatattct tttctatctt cgccataatc ggcgtaaacc
     2401 ttcacctttt gtcccaatgc aatttgggtt aattggtcgg aagttacgta tgctctcaaa
     2461 atcatattgg taacatctcc tattttgaac aatgcctttc ccggtgttgc tagctctcct
     2521 tgttcagcat attttgttaa aacaactcct gatataggcg aggttatata gcatttttgt
     2581 agctgatctt ctatttgttc tatttgtatt tgcaatgctt cattatcacc caaaatacta
     2641 ttgcttgtat tttcgagaga tgattttgtc gcctccaatt gtttttccaa caccttaatt
     2701 tgagcgtttg catcgtctaa ttgtttggtt gtggaagctt ctgcttttac taaattttct
     2761 actcgtttac gttctgtttt tgccgtttca atttgttgtt ctaaggctgc tatttgtttt
     2821 tgaatatcag gacggcgact ttgtagtgct ttcttagaag ctacgagttg caattttttc
     2881 aaatacagtt gagtggtatc aatataacca actcgctgat taaaatttag ttgttgtccc
     2941 tcctcaacat ttagttgcat aatctttccc atggattcgg ttgaaacaat aatttctgtt
     3001 gtttcaaatg ttcctgaagc atccacatct ccatcactat tgctacacga cacaagaaat
     3061 atacttacta ttgttgccaa aagaccaatc cgacaactat ttcggagaaa tatcgcttgc
     3121 tttttatttt ttgttttcat agtttatgat tatttgttga tattgttttt aacctcataa
     3181 agccttttta taagttgtat ttcatgcaat gatttcagtt gtttcgcctg attctcggca
     3241 ttaatctctc gaattaaatc cgcaacagtt aatgtgccat tttctacctt tgagacagca
     3301 accttcttaa tattttcacg taaacgaatt atgtcatcat ccgtatttaa gagtgcttga
     3361 agatttttta tggcgagctt gtcttgttca ttttttaaat ccgtattaaa caaaaaagtt
     3421 tctttttgaa cgtcaatact ttgtttattg atatccagtt tactagtatt attttttgta
     3481 gaatagagtt ctccgagatt ccaagaaaaa cgaattcctc ctatgtagaa aggagcaaat
     3541 tcattggcaa acatattcaa tgctggcttt ccgtatcctc cttgaaaaaa agctgatagt
     3601 ttgggaagat tattggcatg tattatcttt ttttgagatt cgtaatactc actttgataa
     3661 gccaataatg taagctctgg acggttattg gttgtatctc ccaaaataag aagatttttc
     3721 tctggtttac tcagcgttac tttttcatct agttccattt tcgtaaatat cgataacatt
     3781 ctaacatagg caagtttcgt atttacgagt tctgtattac gttgttctac attcaactgt
     3841 tctacctgta tggcatctaa gtccgattga ttcgctactc cattctgttt atatgcctca
     3901 atccttttgt aatgtgtttg taattcatct ttcagcattg tatttaaatg cacttgttca
     3961 tcaatcaaca aaattccaaa atataactga cttacccttt cttttaacgc atataaatca
     4021 acctccactt tttgtttctc gactttagat agcgttttta ccacttcctt ttgtgcatgt
     4081 gtagcacctc catcccacat aagttgagat atttctccta ctagttgata ctgatctttc
     4141 gataatcctt ccatactaat ccccagtcgc tgactaagag cttctggcaa ttcagtaaca
     4201 tccgattgat aggttgcctt agccgatagt agcaacttag gcaaataagc cttggtcgca
     4261 tttgatacgt tatatgctgt ggtgttttct atcagcccat attgctttat aagcgggtaa
     4321 tttatgcgag ctttttccca acattcttcc agtgttacag actgagctga tataagttga
     4381 aacgccatag caataaccga acataaccat atttttttca tatcatattt tatttggagt
     4441 agtatactat attacggtct caaataactc atcacgatgt taacatgttc tattttgcga
     4501 tgagcaagaa tactcttccg ctcattttca tctagttcga ctatacttgc aacaataggt
     4561 aaaaatgtaa acaaagccgc attgagagaa acaatagtaa tgattacatc tgtcaaatca
     4621 acattcctta cccttcctgc ggcaatttcc acttgcaatt cttctcccaa ttgagcaaac
     4681 aaactttctg gataactata taatttttct ttcaatgatt ttatttgttc tggcaatctt
     4741 ggcaattctg atataatcag ttgtggtatc tgcggatttt gactaatcaa atcaaaatgt
     4801 gtttctatta ttttctttaa tttatcaaga aaagacaaat tttctaaatg attcatgtca
     4861 aaaatctgtt gaaaaaaagc tttaaacttc ttttcaaaaa tcgtattaaa taatttttct
     4921 tttgtacgga agtaataatg tactaaagcc tgattgcatc cgacttgttt cgcaatgaga
     4981 gtggttgacg ttgaggcata tccattttct aaaaataaac gttctgctgt ttctaaaata
     5041 atttcttcca ttgagttcac attgttaata ctcatattta attatcctat ttaataaggt
     5101 tattaaataa aaacaaagaa acagcaatat tttaaataat cagcacataa ttgtgttaaa
     5161 ataacttaat tgcaccgata taccacaaaa tttgatgctt agacaaactg atgttttgtt
     5221 ttattagcaa ttttaaccaa tgccagcata acaggaactt ctaccaatac gcctactact
     5281 gttaccaaag tagctcccga ctgcaatccg aacaacacaa tggctactgc caccgatagt
     5341 tcaaaaaaat tactagcacc aataagcgat gctggagcag caatattatg cggtagtttc
     5401 catgctttcg cccagcgata tgccacaaag aaaataaaga atgtttggat aataagaggt
     5461 actgcaatca ataaaatata caaaggattt tgcaaaatca catccccttg aaatgtaaaa
     5521 atgattacca aagtaagcaa taaaccactt atggtagtgg cagaaaactt tttgataaac
     5581 acatcagtaa aataggcgat accttttttt ctgatgatca aattgcgtgt gatataagca
     5641 agcaccaagg gaatgactac aaacaacaaa accgacataa tgagcgtatt ccacggaata
     5701 acgataccag aaacgcctaa taaaagcgtt acaataggaa taaatgctac aataatgatt
     5761 aaatcgttga tggctacttg taccaaggtg taagcaggat tccctttggt taaatgactc
     5821 cacacaaaca ccattgccgt acacggagcc gcaccaagca ataccgcacc tgcaaggtac
     5881 tcttttgcaa ggtcttcggg aatccacagc gaataaataa ccataaaaaa caaccaagca
     5941 aaaccaaaca tggtaaatgg tttgatgagc cagtttacca ccaaggtaat ggtaagacct
     6001 ttgggcattt tgactgcatc taccacactt ttaaagtcaa ttttgagcat tatcggaaaa
     6061 atcattatcc aaataagcac agcaatggga atagacacat tggcgtactc taatagacct
     6121 aaggtttcgg gtactattgg taacaatttg ccg
//
LOCUS       DAOPUR010000019         6051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_12978_length_6051_cov_3.582889, whole genome shotgun sequence.
ACCESSION   DAOPUR010000019 DAOPUR010000000
VERSION     DAOPUR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12978_length_6051_cov_3.582889"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKN90_00590"
     CDS             <1..496
                     /locus_tag="PKN90_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNZ84814.1"
                     /translation="TIIGFEPHSIYAMRLAKKGELRTHHKDKAVLNMLNMNRLVETDL
                     CKVEPSATLGDLVKVIATSKRNLFPVVDKDGVFLGVVLLEEIRNIMFRTELYQRFTVA
                     DLMIAPPSRIFVGEEMEEVMMKFEKTQAWNLPVLDGDKYVGFVSKSTIFSAYRNVLVD
                     FSEE"
     gene            546..2129
                     /locus_tag="PKN90_00595"
     CDS             546..2129
                     /locus_tag="PKN90_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNZ84815.1"
                     /translation="MKLKYLVCFVCFPIVLLAQQADYQKPRLVIGITVEGLTADYLSM
                     FWNGMGNQGFRKIVENGATLSSLQFDYDATDNVSDIATFSTGSQPYLHGVSWSDVYNR
                     NTRKYQFLLQDDAVKSINGVDQLSARGLLVTTLADELKIHSIQRSKVVSIAMNAKESI
                     LQTGHLADIVMWIDDNTGKWSSSTYYNEQLPSWVNRKNEQNAIQNYMEMVWEPLYPIN
                     YYYVTQSRGFTTKSFSYPLKKMTMGKNGVKEFKKTPFANTCIREMAVEAIEQETLGRD
                     IFPDVLLLNFRLQSYPQASDLTVELEDAYVRLDNDIKMILEAVERKIGKEHILVYITS
                     PRKTHYAVSPLLKEKMQLGTFNSYRYMALLNAYLMAIYGQESWVMGFYNGNIYLNRKI
                     IDEKNLSLKEVQERAMEFVSQLPGVMNVVNSVALEQQFALRNSLRQSYNKQSSGDILF
                     TLYPGWVEVDKNDNPTGFYNVLGNSVPFYFYGWKIQPQTIATPYAIIDATATLAKWLQ
                     IPLPNASNGVLLPIVLSAE"
     gene            2424..3032
                     /locus_tag="PKN90_00600"
     CDS             2424..3032
                     /locus_tag="PKN90_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ84816.1"
                     /translation="MDKIKQEILKTATNAFVKKGIRSMSVDDICDDLRISKKTFYSYY
                     RQKEALVMAVMETFTTHHQEYHCMLQNQEFTNVIDEILWMKTLIKNGKEEKKHERFFN
                     DLIKYYPSIYNTFSVENRQYTEQMFLTQIEKGIAQGVFREDIHVQFTAQSLTVSLQYF
                     SNMSIKHPRKYTKLGIIDFMVDSYLRMVCNEKGLQYYTSLLT"
     gene            3052..4341
                     /locus_tag="PKN90_00605"
     CDS             3052..4341
                     /locus_tag="PKN90_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ84817.1"
                     /translation="MKRKLFLLFASFFLGYSVHSIAQESLHISIDEAKEYALNYNKTI
                     QKANLAIQKADYAKWQSIASLLPKVDASLGYNNYLGHKMSLMGTTIAMNPEGSFTAQA
                     SIALSGMQVVGVQLSEIAQQMSQTSALSDALSLQTNVTNAYYAILVSEESKKIVEGNL
                     ENLRKLYNSSLNLVKVGMAEQTDADQLAVQVGMIENTIRMAERNVELSYNTLRLLLGV
                     DNNTPLSLKNTLKDFTNKESANELLATSFDMTQNYTVTLLQQTIEMNQKQVRLNQWAY
                     APMLTGFYQYTKKTYFDKAVGFNMTPPNLVGVTISIPVFSSGERYAKVKQAQFDLQTA
                     KLNKEQTVDALLMEEKQLRYNLTSAIESYELQLKNVALYERILTNATQKYEQGMISST
                     ELSTISNNTLSTQSQYISALMDMLMAKTKLQQLINKL"
     gene            4369..5391
                     /locus_tag="PKN90_00610"
     CDS             4369..5391
                     /locus_tag="PKN90_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ84818.1"
                     /translation="MKKGIKISMLMLAVIALLNACTPKAEKKQEEEIRVENVKTAVLE
                     TQEIARKIDFSTTLEPYNKVMVAPTMAGRIDKIHVEVGSKVQANQLLVEMDKTTYLQT
                     KVSFDNLAVDFQRISTLYNSGNIAQQTYDQTKAQYESLKTNVQNLERNTFLRAPFSGV
                     IAAKNYENGELYAGTPILLLVDMANLKAHINIPESYYPSIKEGMNVAITSDVFTDKEF
                     KGIIEIVSPVIDATTHTFQVQIKISNAGGVLRPGMFARIGVDFDKINAKVVPYQAVLK
                     LQGSNERYVYVNKNGVAKRVKVQMGKRYDDKIEILSDELKEGDQLVVVGQARLVDGVK
                     VNVVNE"
     gene            5407..>6051
                     /locus_tag="PKN90_00615"
     CDS             5407..>6051
                     /locus_tag="PKN90_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ84819.1"
                     /translation="MSIYKSSVNKPITTILIFVGVIIMGLYSLIQLPIDQFPEIEIPS
                     VTVMTSYPGANGTEVETNVSKVLENSLNSVDGLKEIYSTSKDNLSLVTLEFEWGANLD
                     EALNDVRSSIDLVYDALPDGASRPMIIKLNASMMPVMQYAVTAEDSYPGIKKIIEDNL
                     ITVLNRIDGIGNISLLGSPQRYVYVDIDPTKLNSFGLSLETVGSAIANNNMNLAS"
BASE COUNT         2102 a          918 c         1154 g         1877 t
ORIGIN      
        1 taccattatt ggttttgaac cccatagtat ttatgctatg cgtttagcta aaaaaggtga
       61 gttgcgtaca catcataagg ataaagctgt attgaatatg cttaatatga accgtttagt
      121 cgaaactgat ttgtgtaaag ttgagccatc ggctactttg ggtgatttag taaaagtaat
      181 agctacctct aagcgtaatt tatttccagt agtagataaa gatggcgttt ttttaggagt
      241 cgttttatta gaagagatac gcaatattat gttccgaacc gaattgtatc aacgttttac
      301 tgttgctgat ttgatgattg ctccgccatc tcgtattttt gtaggagaag agatggaaga
      361 agtaatgatg aagtttgaaa aaacacaagc atggaatttg ccagtattgg atggagataa
      421 atatgttggt tttgtttcaa aatctactat ttttagtgcg tatcgcaatg tgttagttga
      481 tttctctgaa gagtaatttg ttcaaagaac atgtctttct atacatgtag taaaataata
      541 attgtatgaa attgaaatat cttgtctgtt ttgtgtgttt cccaattgta ctgttggcac
      601 aacaagccga ttatcaaaag cctcggttgg ttattgggat tactgtagag ggattaactg
      661 ctgattattt atccatgttt tggaacggaa tgggaaacca aggctttcgt aaaatagttg
      721 aaaatggagc tacattatca tctcttcagt ttgactacga tgctactgat aatgtgtctg
      781 atatagctac ctttagtaca ggttctcagc cttatttgca tggtgttagt tggtctgatg
      841 tatataacag aaatacccgt aagtaccaat ttttattaca agatgatgcc gttaaatcaa
      901 ttaatggcgt ggatcaactt tcggcacggg gcttgttagt taccacttta gctgatgaat
      961 tgaaaataca ttcaattcag cgatcaaagg tagtgtcaat agcaatgaat gcaaaagaga
     1021 gtatattaca aacaggacat ttggcagata tagtaatgtg gattgatgat aacactggta
     1081 aatggtcaag ctctacgtat tacaatgaac agttgccatc ttgggtaaat cgcaagaacg
     1141 aacaaaatgc catacaaaac tatatggaaa tggtgtggga acccttgtat ccaattaatt
     1201 attattatgt aacacaatca aggggattta ccacaaaaag tttttcttat ccgttgaaaa
     1261 aaatgacaat gggaaaaaat ggggttaaag aatttaagaa aactcctttt gccaatacct
     1321 gtattcgcga aatggctgta gaagctatag agcaagaaac tttggggagg gatattttcc
     1381 ctgatgtatt gttgcttaat tttcgtttac aatcctatcc tcaagcaagc gatttaactg
     1441 tcgaattaga agacgcatat gtacgattag ataatgatat caaaatgatt cttgaagcag
     1501 tagaaagaaa aattggcaaa gaacatatcc ttgtatatat cacctctcct cgcaaaactc
     1561 attatgctgt gtctccttta ttaaaagaaa aaatgcaact cggcacattt aactcgtatc
     1621 gctatatggc tttgttgaat gcttatctaa tggcgatata cggtcaggaa agttgggtaa
     1681 tgggttttta caacgggaat atttatttaa atagaaaaat aatagacgaa aagaatttga
     1741 gtttaaaaga agtgcaagaa cgagcgatgg agtttgtatc acaattgcct ggtgtgatga
     1801 atgtggttaa ttcagtcgct ttggagcaac aatttgcgtt aagaaattcg cttcgacaat
     1861 cctacaacaa gcaaagctca ggagatatat tatttacctt atatccaggt tgggttgagg
     1921 tagataaaaa cgataaccca actggtttct ataatgtatt aggcaatagt gttccttttt
     1981 atttttacgg atggaagata cagccacaaa ccatcgctac accgtatgcc ataatagatg
     2041 ctacagcaac acttgccaaa tggttacaga ttcctttgcc caacgctagt aatggtgtgt
     2101 tgttgccaat tgttttatct gcagaataga atttttcttt cctaagttta ttgtcaaatt
     2161 tggtataatt tatcatacta atgatttaaa tttcaattct taatgaggtt tcactctaaa
     2221 agtgctattt tttatccaat ttttgttctt cgaaatggaa acttggaaac tgtaaccaat
     2281 atttcgtttc cattaattgt ttgataatca gtgtttagta atttgttaca ataatttaac
     2341 attaatggtt gtggtaattt attgttcgta ttctatcttt gcgtcctatt tcagagtgaa
     2401 agagacaaat gtatttttag tatatggaca aaattaaaca agaaatctta aagactgcta
     2461 ccaatgcttt tgttaaaaaa ggtattagaa gtatgagcgt agatgatatt tgtgatgatt
     2521 tacgtatttc taaaaaaacc ttttatagtt attacaggca aaaagaagca ttagtaatgg
     2581 ctgttatgga aacgtttacc acacatcacc aagagtatca ttgtatgctt caaaaccaag
     2641 agtttacgaa tgtgattgat gagattttgt ggatgaaaac attgataaaa aacggaaaag
     2701 aggaaaaaaa acatgagaga ttttttaatg atttgataaa atattatccc tcaatataca
     2761 ataccttttc tgtagaaaac cgacaatata cagaacaaat gtttcttact cagatagaaa
     2821 aaggaatagc acaaggtgtt tttagagaag atattcatgt tcaattcacg gctcagtcgt
     2881 taactgtttc cttgcaatat tttagtaata tgtccattaa gcatcctcgc aaatacacca
     2941 aacttggcat cattgatttt atggttgata gttatttgcg tatggtgtgc aacgaaaaag
     3001 gattacagta ttatacatca ttattaacat aaaaaaataa aaacatatca gatgaaaaga
     3061 aaattatttc tattgttcgc ctcattcttt cttggctatt cggtgcatag tatagctcaa
     3121 gaatctttgc atataagcat tgatgaggca aaagagtacg ctctaaacta taataaaaca
     3181 attcaaaaag ccaatttagc cattcaaaaa gcggattatg ccaaatggca gtctattgct
     3241 agtttgctac caaaagtgga tgcatcattg ggctataata attatctggg tcataaaatg
     3301 tcattaatgg gtacaacaat tgctatgaat cccgagggga gttttaccgc tcaggcttcc
     3361 attgcactca gtggtatgca agtagtagga gtgcagctga gcgagatagc tcaacaaatg
     3421 tcgcaaactt ctgcgttgtc ggatgcatta agcttgcaaa caaatgtaac caatgcgtat
     3481 tatgccatat tagtgtctga agaatcaaaa aaaatagttg aaggtaattt agaaaattta
     3541 cgcaaactat acaattcttc gcttaatctt gtaaaagtgg gcatggcaga acaaacagat
     3601 gctgaccaat tagcagtgca agtaggtatg attgaaaata ccatacgtat ggcagaacga
     3661 aatgtggaat tgtcatacaa taccttgagg ctgttattgg gtgttgataa taacacgcca
     3721 ctttcattga aaaatacgct aaaagatttt acaaacaaag aatctgcaaa tgaactattg
     3781 gctacatcgt ttgatatgac acaaaattat acggttacac tgttgcaaca aactatcgaa
     3841 atgaatcaaa agcaagttcg attaaaccaa tgggcgtacg caccgatgct cactggtttt
     3901 tatcaatata cgaagaaaac atattttgat aaagcggttg gctttaatat gactccacct
     3961 aatttggtag gagttaccat tagtattcct gtgttttcaa gcggtgaacg ttatgccaaa
     4021 gtaaaacaag ctcaatttga tttgcaaact gccaaattaa acaaagaaca aacagtggat
     4081 gccttgttaa tggaagaaaa acagttgcgt tataatttga cttcagctat tgaatcgtat
     4141 gaattacaac taaaaaacgt ggctttatac gaacgtattt taacaaatgc tactcaaaaa
     4201 tacgagcagg gaatgatttc gagtactgaa ttgtcaacta tcagcaataa tacattatct
     4261 actcaaagtc aatatatcag tgctttaatg gatatgttaa tggcaaaaac taaattacaa
     4321 caattaataa acaaacttta aactaaacca atcttaatag ttaccaagat gaaaaaagga
     4381 ataaaaatat caatgctcat gttggctgta attgctttac tgaatgcatg cacgcctaaa
     4441 gcagaaaaaa aacaggaaga agaaattcgt gtcgaaaatg ttaaaacagc tgttcttgaa
     4501 acgcaagaaa tagctcgaaa aattgatttt tcaaccacat tagagcctta caataaggta
     4561 atggttgccc ctactatggc tgggcgtatt gataaaatac atgtagaagt tggttctaag
     4621 gtgcaagcca atcaattgtt agtagaaatg gacaaaacta cttacttgca aacaaaagtg
     4681 agttttgata atttggcagt cgattttcaa cgtatttcca cgttgtataa ttcaggtaat
     4741 atagcacagc aaacgtatga tcaaacaaag gctcaatacg aatcactaaa aacaaatgtt
     4801 cagaatttag aacggaatac ctttttaaga gcaccattct cgggtgttat agctgctaaa
     4861 aattatgaaa atggagaatt atatgctgga acacctattt tacttttagt agatatggcc
     4921 aatttgaaag cacatattaa tattcccgaa agttactatc catccattaa agaaggtatg
     4981 aacgtggcaa ttacatcgga tgtgtttact gacaaagaat tcaagggaat aatagaaatt
     5041 gtttcgcctg tgatagacgc tactacacat acttttcagg tgcaaattaa aatctcgaat
     5101 gctggcggag tattacgacc aggaatgttt gcacgtattg gagttgattt tgataaaatt
     5161 aatgctaaag tagtacctta tcaagcagtc ttaaaactac aaggctctaa cgaacgctat
     5221 gtatatgtta ataagaatgg agtagcgaaa cgtgtaaaag tacaaatggg gaaacgctat
     5281 gatgataaaa tagaaatttt atctgatgaa ctcaaagagg gagaccaatt agtggtagtg
     5341 ggacaagctc gattagtaga tggtgtaaaa gtaaatgttg taaacgaata gaaatttaac
     5401 aataccatga gtatatataa atcatctgta aataagccta ttacaacaat attgattttt
     5461 gttggggtta ttattatggg gttgtattca ctaatacaac taccgattga tcagtttccg
     5521 gaaatagaaa taccgtcagt aacggttatg acttcgtatc ctggagcaaa cggaacggaa
     5581 gtagaaacaa atgtgagcaa ggtcttggaa aatagcctta actctgttga tgggcttaaa
     5641 gaaatttatt caacatccaa agataatttg tcgttagtaa cacttgagtt cgaatgggga
     5701 gcgaatttag atgaggcact taatgatgtg cggtcttcta tcgaccttgt gtatgatgca
     5761 cttccagatg gagcttcacg acctatgatt atcaagctta atgccagcat gatgcctgtt
     5821 atgcaatatg ctgtaactgc cgaagatagc tatcctggta taaaaaaaat aatcgaagat
     5881 aacttaataa ccgtattaaa tagaatagat gggataggaa atatctcttt attaggctcc
     5941 cctcagcgtt atgtatatgt agatatagat ccaacgaaac taaattcatt cggattaagc
     6001 ttagaaacgg taggttctgc tattgccaat aacaacatga atttggcttc a
//
LOCUS       DAOPUR010000020         6002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_13158_length_6002_cov_2.865310, whole genome shotgun sequence.
ACCESSION   DAOPUR010000020 DAOPUR010000000
VERSION     DAOPUR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13158_length_6002_cov_2.865310"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PKN90_00620"
     CDS             <1..654
                     /locus_tag="PKN90_00620"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNZ84820.1"
                     /translation="SASIIGNKLIPIMGINMGRLGFLADVPNNSIAEMVDEIFSKGYK
                     TENRTLLKLSTSSKDIFQDDTALNEIAVLKLDSSSMIMIHATINKEYFCSYQADGLIL
                     STPTGSTAYALSVGASILDPTNQSFILAPVAPHSLNLRPFIFPDTWEVDFEIESRNNS
                     FLVSIDGRSYMMDQATKLNIKKAPYSIIIAKRLEDTYFQTLRNKLMWGADTRHKKPF"
     gene            656..961
                     /locus_tag="PKN90_00625"
     CDS             656..961
                     /locus_tag="PKN90_00625"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNZ84821.1"
                     /translation="MLAYNITFTMDYNVVNFWEQWIVQKFFPMMLETSLHSPKIFKVY
                     TNATEQRIFTVQFLVESFSDYDSFQKDNEAAIIKQLAKTFGNDVVCFTSFIQEISLE"
     gene            962..1540
                     /gene="ruvC"
                     /locus_tag="PKN90_00630"
     CDS             962..1540
                     /gene="ruvC"
                     /locus_tag="PKN90_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNZ84822.1"
                     /translation="MSDSLTYKDAIILGIDPGTTIMGYGLLQIKGGKPTMLAIGVLDL
                     KKYPDHYSKLQRIFARVLSLVDEFKPTALAIEAPFFGKNVQSMLKLGRAQGAAIAAAL
                     YRDIPVHEYAPLKIKMAITGSGAASKEQVARMLQRFLNLSEQDMSLQLDATDGLAAAY
                     CHFLQLNKPFSSSKKTSWKAFAQQNSHRVKGL"
     gene            complement(1545..2726)
                     /locus_tag="PKN90_00635"
     CDS             complement(1545..2726)
                     /locus_tag="PKN90_00635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNZ84823.1"
                     /translation="MYKQIFLKPKKEESLKRFHPWVFSGAISHTDKPVEEGEIVEIYD
                     SAKNFIALGHSQIGSIAVRVLSFEKEEVNENFWVKRIRESASARTSLGVSHSTTHSIY
                     RLVHGEGDLLPGLIVDVYGKTAVMQAHSIGMHVHRHAIAEAIIHVLSPNVSAVYYKSS
                     GTLPYKADIDVEDGFLVGNSTEGTALENGLAFYIDWLKGQKTGFFIDQRDNRSLLEKY
                     ANGKSILNMFCYTGGFSVYALRGGANMVHSVDSSAKAIDLTNKNVTLNFPNDTRHTAF
                     ATDAFKFLAEMDNAYDIIVLDPPAFAKHRDALKNALQGYKRLNAKAIEKIKPGGILFT
                     FSCSQIVSKEQFRLAVFSAAAITGRHVRILHQLTQPADHPISIYHPEGEYLKGLVLMV
                     E"
     gene            complement(2737..3321)
                     /locus_tag="PKN90_00640"
     CDS             complement(2737..3321)
                     /locus_tag="PKN90_00640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ84824.1"
                     /translation="MKHPTHISAELMATLPTLSYPEKKIIIVDTLEKLEEAVHKLNTQ
                     KLIGFDTETKPSFTKGVINKVALLQLASEDVCYLFRLHLIGLPHSLMAILANPSIIKI
                     GIALRDDFSGLRKWNNAKPQGFIDIQQEIHKVGIQELSLQKIYAILFARRISKNQRLS
                     NWESEELSEAQQHYAAIDAWACLDIYHEIKTYIE"
     gene            complement(3350..3955)
                     /locus_tag="PKN90_00645"
     CDS             complement(3350..3955)
                     /locus_tag="PKN90_00645"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HNZ84825.1"
                     /translation="MHTTHLSTQAIEFITVSLAYCTALEDDMSEHTTMYFDKLSKILP
                     LLYLKASLVDTTNSNEVYEDVEKFVTEHEYEFIQAKIASLFGAYDNYLEVSEEVDTPI
                     MGSISEDLADVYQDIKDLVMNAKVGNPEVVSIAVKICIENFQQYWGQKLLSALKQIHS
                     ILYNNELENVTDESILQKNTVKKQTFSDFLRDEEDDYSNLL"
     gene            4113..4661
                     /locus_tag="PKN90_00650"
     CDS             4113..4661
                     /locus_tag="PKN90_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNZ84826.1"
                     /translation="MKEIIEESEILAMLASEQTKRKAFSWIMKTYQQKMYYHIRKMLI
                     NHDDTDDVLQNTFLKAWLHIASFRGESSLSTWLYRIATNETLDFLRSKKQTNEYVDIE
                     DTLALSLQDTSYFSGNEIELKLQKAILLLPEKQRLVFNMKYFDDITYEQMSEILETSV
                     GALKASYHLATKKIEKLILLMD"
     gene            4705..5049
                     /locus_tag="PKN90_00655"
     CDS             4705..5049
                     /locus_tag="PKN90_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84827.1"
                     /translation="MEINDNSRNEVGNKVPFTTPDNYFEEFSAKIEQLLDKQEESNQV
                     INLSLWQRVQPYVYLAAMFIGLYVSITTFVKPSIQQKQKEQELVELAIQKELLLDEID
                     DYALYELLSYNN"
     gene            5140..5643
                     /locus_tag="PKN90_00660"
     CDS             5140..5643
                     /locus_tag="PKN90_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84828.1"
                     /translation="MKKTVVACLMLLGGITMIVAQTDNRAEMRAAKMQEMQAQKIAFI
                     KEKVSFTDKESAEFFPLYQEFEQQRMELNRKSRPQGKGFKAKNTELSEQEKEAVMDNF
                     IQNRVETANLEKAYYEKFKKILPASKLFEVYQADREFKQELLQDFRNRGAQNQDKKSR
                     ENKSPKK"
     gene            5866..5941
                     /locus_tag="PKN90_00665"
     tRNA            5866..5941
                     /locus_tag="PKN90_00665"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5899..5901,aa:Lys,seq:ttt)
BASE COUNT         2044 a          958 c         1029 g         1971 t
ORIGIN      
        1 tctgcttcta ttattggaaa taaattgata cctataatgg gtattaatat gggtcgtttg
       61 ggctttttgg ctgatgtccc taataattcg attgctgaaa tggtggatga aatttttagt
      121 aagggatata aaactgagaa tagaacgttg ttaaaattgt caacttcatc taaagatatt
      181 tttcaggatg ataccgcatt aaatgaaatt gctgtattaa aattagactc atcatcaatg
      241 attatgatac atgcaacgat taataaagaa tatttttgtt cgtatcaagc agatgggtta
      301 attctttcta cgccaactgg atcgacagcc tatgcattga gtgtaggagc ttctatttta
      361 gaccctacta atcagagttt tatactagca ccagtggctc ctcatagttt aaatttacgt
      421 ccctttattt tccctgatac atgggaggta gattttgaaa tagaaagtag aaataatagt
      481 tttttagtat ctattgatgg tcgttcgtat atgatggatc aagctactaa gctgaatatt
      541 aaaaaagctc cttattctat tataatagca aaaagattgg aagatactta ttttcaaact
      601 ttgcgaaata aattaatgtg gggagcagat actcgtcata aaaaaccttt ttagtatgtt
      661 agcctataat attacattta caatggatta caacgttgtt aatttttggg aacaatggat
      721 tgtccaaaaa ttttttccta tgatgcttga aacgtcttta cattcaccaa aaattttcaa
      781 agtttatacc aatgcaacag aacaacgcat ttttaccgtg caattccttg tagagtcttt
      841 ttctgattat gatagtttcc aaaaagataa tgaggctgcg ataatcaaac aattggcaaa
      901 aacatttggt aatgatgtag tatgttttac gtcttttatt caggaaatat cattagagta
      961 aatgtcagac agtttaacat ataaagatgc cattatttta ggaattgacc ctggtactac
     1021 tattatgggg tatggtttgt tacaaattaa aggtggtaaa ccaacgatgc ttgctattgg
     1081 tgtgttagat ttaaaaaaat acccagatca ttattctaaa ttgcaacgta tttttgctcg
     1141 tgtattatct ttagtggatg agtttaaacc aactgcatta gctatagagg ctcctttttt
     1201 tggtaaaaat gtgcaatcaa tgctcaaact aggtagagcc caaggagccg caatagcagc
     1261 agctttgtat cgcgacattc cagtacatga atatgcccca ttaaaaataa aaatggctat
     1321 aacaggttcg ggagcagcat ctaaagagca ggttgctaga atgttacagc gttttttaaa
     1381 cttgtcagaa caagatatgt cgctacaatt agatgcaact gatggtttag ctgcagctta
     1441 ttgtcatttt ttacaactca acaaaccctt ttcttcttct aaaaaaacat cttggaaggc
     1501 ttttgctcaa caaaattcac atagagtaaa aggcttataa ctgtctattc taccatcaaa
     1561 accaatcctt taagatattc tccttcagga tgatagatgc taatggggtg gtcagctggt
     1621 tgggttaatt gatgtaaaat acgcacatgt ctacctgtaa tagcggctgc actaaatacc
     1681 gctaatctaa attgttcttt gctcacaatt tgtgaacaag aaaaagtaaa caatatgcct
     1741 cctggtttaa ttttttcaat tgcttttgca tttaggcgtt tatagccttg taaggcattt
     1801 tttaatgcgt ctcggtgttt ggcaaacgct ggggggtcaa gaacaatgat atcatacgca
     1861 ttatccattt cagctagaaa cttaaatgca tctgtggcaa aagctgtatg acgagtatcg
     1921 tttgggaaat ttagcgttac gtttttattt gttaagtcta ttgccttagc tgaactatcg
     1981 accgaatgta ccatgtttgc acctcctctt aaagcatata cagaaaaacc ccctgtatag
     2041 caaaacatgt ttaaaataga tttgccattt gcgtattttt caagcaaaga gcgattatct
     2101 cgctgatcga taaaaaagcc tgttttttga cctttcagcc agtcaatata aaatgctaat
     2161 ccgttttcta atgcagtgcc ttctgttgaa ttgccaacca aaaatccatc ttctacatca
     2221 atatcagctt tgtagggtaa tgttcctgac gatttataat aaacagcact gacgtttgga
     2281 gacaatacat ggataatggc ttctgctatg gcatgtctat gtacatgcat tcctatagaa
     2341 tgagcctgca ttacggctgt ttttccgtaa acatctacta ttaagcctgg aagcaaatcc
     2401 ccttctccat gcactagtcg atatattgag tgagttgtgc tgtgcgaaac acccaacgag
     2461 gtacgtgcag aagcactttc acgaatgcgt tttacccaaa aattttcatt aacttcttct
     2521 ttttcaaaag acagtacacg aactgcaatg cttccaattt ggctatgacc taacgcaata
     2581 aagtttttag ctgaatcata aatttccact atctctcctt cttctacagg cttgtcggta
     2641 tgagaaatag cccctgaaaa tacccaagga tgaaaacgtt ttaatgattc ttctttcttt
     2701 ggttttaaaa atatctgttt gtacataagc tctattttat tcgatataag ttttaatttc
     2761 gtgataaata tccaagcatg cccatgcgtc tatggctgca tagtgttgtt gtgcttccga
     2821 caattcttcc gactcccaat tggatagtcg ttgatttttc gaaattctac gagcaaataa
     2881 gatggcatat attttttgca aactaagttc ttggataccc accttatgaa tctcttgttg
     2941 aatatcaata aaaccttgag gtttagcgtt gttccatttg cgtaatccac taaaatcatc
     3001 acgtaatgct atcccaattt ttataataga tggattagct aaaatagcca ttaaagagtg
     3061 gggtaatcca atgaggtgta agcgaaataa ataacaaaca tcttcagagg ctaattgtag
     3121 taatgctact ttattgatta ctccttttgt aaaggatggt ttcgtttctg tgtcaaaacc
     3181 tattaatttc tgagtattta atttatgtac agcttcttct aatttttcta gcgtatcaac
     3241 aatgataatt tttttttcgg ggtaagaaag tgtgggaagg gttgccatta attcggctga
     3301 aatatgtgtc ggatgtttca tcttacattc acttattagg gataaaaact tacaataaat
     3361 tgctgtagtc atcttcttca tcacgcaaaa aatcgctaaa agtctgtttt tttaccgtat
     3421 ttttttgtaa tatagactca tctgttacat tttctaattc attattgtat aaaatactgt
     3481 gaatctgttt cagtgcacta agcaactttt gaccccagta ttgctgaaag ttttctatac
     3541 atatttttac agcaatactt actacttcag gatttcctac tttagcattc attactaaat
     3601 cttttatatc ctgatatacg tctgctaaat cttcagatat gcttcccatg ataggtgtat
     3661 caacttcttc tgatacttct aaatagttat cataggctcc aaataaagag gctatttttg
     3721 cttgtataaa ttcatattca tgttctgtaa caaatttctc aacatcctcg tatacttcgt
     3781 tactgtttgt tgtgtctaca agtgatgctt ttagatataa taaaggtaga atttttgaaa
     3841 gtttgtcaaa gtacatcgtt gtatgttcag acatatcgtc ttctaatgca gtacagtagg
     3901 ctaaactaac agtaataaat tcgatagctt gtgttgataa atgagtagta tgcataaaga
     3961 ttaataagta attaaacctt ttatattttt ataagtccaa taaaaaagcg tattttagta
     4021 gcctctttat aaatattttg ggaaacaaag atacgaataa taaaaaaata gaatgcatta
     4081 ttcatgaata aacacacaca aatttatttt gaatgaaaga aattattgag gaatcagaga
     4141 ttttagccat gttagcctct gaacaaacaa aacgaaaagc tttttcgtgg ataatgaaaa
     4201 cataccagca aaaaatgtat tatcacattc gaaaaatgtt gataaatcat gatgataccg
     4261 atgatgtact tcaaaataca tttttaaaag catggttgca tatagcctct tttagaggag
     4321 agtcttctct gtcaacttgg ctttatcgca tagcaaccaa cgaaacattg gattttttac
     4381 gttcaaaaaa acagacgaat gaatacgttg atattgaaga tacattagcc ttatcgctac
     4441 aagatacttc ttatttttcg ggaaatgaaa ttgaattaaa actacaaaaa gcaattttat
     4501 tgttgccaga aaaacaacgt ttggtattta atatgaaata tttcgatgat ataacatatg
     4561 aacaaatgtc tgaaatttta gagacttctg ttggggcttt aaaagcttct tatcatttag
     4621 ctacaaaaaa aatagaaaaa ttaattttac taatggatta aaccatttga aatatcaatc
     4681 gtcaaagtaa cagaaaacaa tagtatggaa ataaatgata acagtagaaa tgaggtaggg
     4741 aataaagttc catttacaac gccagataat tattttgaag aattttctgc taagatagaa
     4801 caattactag ataagcaaga ggaaagtaat caagtaataa acttaagcct ttggcaacgt
     4861 gtacaacctt atgtgtattt agcagccatg tttattggat tgtatgtatc tatcactacc
     4921 ttcgtcaagc cctccatcca acaaaaacaa aaagaacaag aattagtgga gcttgctatt
     4981 cagaaagagt tattattaga tgaaattgat gattatgctt tatatgaact tttatcgtac
     5041 aataattaat atagaatatc gcataatacg taatatagaa cctagaaata tttgtgcaca
     5101 aaatacatct ccagtgtaaa ccaaaaaatg aaaatgaata tgaaaaaaac agtagtagca
     5161 tgtttaatgc ttttgggcgg aataactatg atagttgccc aaacagacaa tagagcagaa
     5221 atgagagcag ccaaaatgca agaaatgcaa gcacaaaaga ttgcttttat caaagaaaag
     5281 gtaagtttta cggataaaga atcagctgaa ttttttccgt tgtaccaaga gtttgaacag
     5341 caaagaatgg agcttaatcg caaatcaaga cctcagggga aaggctttaa agcaaaaaat
     5401 actgagctga gtgagcaaga gaaggaggct gttatggata attttatcca aaatagagta
     5461 gaaacagcta atcttgaaaa agcatattat gagaagttta aaaaaatctt accagcatca
     5521 aaattgtttg aagtatatca agcagataga gaatttaaac aagaattgct acaagatttt
     5581 aggaataggg gagcacaaaa ccaagataaa aaatcaagag agaataaatc ccctaaaaaa
     5641 taataattaa gaatgttatg aaaatgtgtg tatggaaaag ggaagtaaaa tctttccttt
     5701 tccatttttt ttaaaattat ttatttacca taaatcgaac attattaagt gattgacaat
     5761 agtaggacat aaatatagtg atatcaaaat ctaaaaaagc agttgtttac ttgcgaaata
     5821 aaaaatataa ctttatcttt gcaccgcttt tgtagaaaaa gataagattc gctagctcag
     5881 caggtagagc acatcccttt taaggatggg gtcctgggtt cgagccccag gcggatcacc
     5941 aaaagccaga ttcgctagct cagcaggtag agcacatccc ttttaaggat ggggtcctgg
     6001 gt
//
LOCUS       DAOPUR010000021         5982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_13214_length_5982_cov_2.942298, whole genome shotgun sequence.
ACCESSION   DAOPUR010000021 DAOPUR010000000
VERSION     DAOPUR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13214_length_5982_cov_2.942298"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKN90_00670"
     CDS             complement(<1..541)
                     /locus_tag="PKN90_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ84829.1"
                     /translation="MKTINKLKMTILVVLSVMNLTWLQAQAPSQSDAVMLQGFYWDSQ
                     SETSWTKLTTMASEIATNFDMVWLPPSSLSEGGVGYHPRQWNNQYSTWGSPDNLKTLI
                     STLKAGGCRAIADIVVNHRGGKNTWNDFYEDDFGSYGKFQLTSAHICKDDECVSKGYA
                     ATGANDTGYDVECGARGGYC"
     gene            856..1401
                     /locus_tag="PKN90_00675"
     CDS             856..1401
                     /locus_tag="PKN90_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HNZ84830.1"
                     /translation="MIILFFVTGGISAADLITLSVNENPIITGFVEDSQLAGAGQVVY
                     TKGTSPYEFFLQDQNKYSHTGYFDGIEIGNHTVQVVDANMCKATFSFYLDIPIIPDLF
                     FTPNGDGVNDVWTIKNIHLVSHSVYIYDRFGKLLYTYEDNFDGWDGTYQGNPLPNTDY
                     WFLINIKPINKQLTGHFTLKR"
     gene            complement(1451..2716)
                     /gene="ilvA"
                     /locus_tag="PKN90_00680"
     CDS             complement(1451..2716)
                     /gene="ilvA"
                     /locus_tag="PKN90_00680"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNZ84831.1"
                     /translation="MNESRNSYFPQIADISKAYQRVKNVVKRTPLEYNANLSEEYQAQ
                     IYLKREDLQIVRSYKIRGAYNKMSCLSQEELERGVVCASAGNHAQGLAYACNKLRIKG
                     TIFMPAPTPKQKVRQVRMFGKEYVEIVLTGDTFDDAQFEADVFCRANNMVYIPPFNSK
                     KIIEGQGTIAIEILEDADQEIDFMFIPVGGGGLSSGVGAYFKQVSPHTQIIGVEPLGA
                     PAMYESLRTDNVVTLGTIDKFVDGAAVKKVGHLNFPICKEVLKEVALVPEGKVCSCIL
                     KLYNEEAIVVEPSGVLSIAVLDQYREQIKGKTVVCILSGSNNDITRMEEIKERSLLYE
                     GLKHYFIINFPQRAGALREFLNDVLSPTDDITTFEYTKKINREDGPALVGIEIQQKED
                     YNGLIDRMNKKGIRYQLLKDNPDVYRLLI"
     gene            3107..5323
                     /gene="pflB"
                     /locus_tag="PKN90_00685"
     CDS             3107..5323
                     /gene="pflB"
                     /locus_tag="PKN90_00685"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HNZ84832.1"
                     /translation="MNFKNGKWNNAVDVRDFVSLNITPYDGDSGFLCSPTQRTLDLWQ
                     ICKDAISEERANNGVRSIDNELVSTITSHAPGYIDKEKELIVGLQTDQLLRRAMKPYG
                     GIAVVEKACSENGVEVSPFVKEVFTKYAKTHNDGVFDVYTPEIRTFRSLGVLTGLPDN
                     YARGRIIGDYRRVALYGVNFLIQQKKKDLESLLGPMSDERIRLREEVSEQIKALYAII
                     EMGKSYGFDLSRPAQNAIEAVQWVYMGYLAAVKEQDGAAMSLGNVSSFLDIYIEQDIA
                     EGKISEEFAQELIDQFVMKLRMVRHLRMDAYNEIFAGDPTWVTESIGGALADGRHKVT
                     KTSYRFLQTLYNLGPSPEPNMTLLWSEKLPENFKKFCAQVSIDTSSIQYENDDLMQST
                     RCSDDYGIACCVSYQEIGKQIQFFGARTNLAKTLLLAINEGRCELSGQQVLTGIPKLK
                     SEVLDYEEVMEVFKFTMKEVARVYSETMNIIHYMHDKYYYEKAQMALIDTNPKINLAY
                     GVAGLSIVADSLSAIKHAKVTPIRDEHGVAKSFKIEGEFPFYGNDDDRVDAIAVEVPA
                     YFNQLLKKLPTYKNAEPTLSILTITSNVVYGKKTGATPDGRAHGVPFAPGANPMHGRD
                     HSGAIASLSSVSKLDYSEALDGISNTFSIVPKSLGPTDEERADNLVSIIDGYFNKEAH
                     HLNVNVLNREMLEDAMEHPENYPQLTIRVSGYAVNFVRLTRAQQQEVISRSFFESL"
     gene            5341..>5982
                     /gene="pflA"
                     /locus_tag="PKN90_00690"
     CDS             5341..>5982
                     /gene="pflA"
                     /locus_tag="PKN90_00690"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HNZ84833.1"
                     /translation="MINVHSVESLGTYDGPGIRLVLFLQGCNFKCLYCANPDTIEPKT
                     GRPYGAEDILKMARNQRPFFGKKGGVTVSGGEPLLQARALIPIFKALKEDGFNTCIDT
                     NGSVLTDAVKELLTYTDLVLLDIKQMDEEAHQAITGVKNHLTLRFATYLAEIGKPTWI
                     RYVLVPGYTDQQEHLHQLGAFVKDMKNVEKIEIQPYHKLGKHKYEALGWQYQLE"
BASE COUNT         1801 a         1072 c         1163 g         1946 t
ORIGIN      
        1 cgcaatatcc cccccgcgca ccacattcta cgtcgtatcc agtatcattg gctccagtag
       61 cagcgtatcc tttgcttaca cactcgtcat ctttgcagat atgagcagaa gtaagttgaa
      121 atttaccata cgagccaaaa tcatcctcat aaaaatcatt ccatgtgttt ttaccgcctc
      181 tatggtttac tacaatgtcg gcaatagctc tacaccctcc cgcttttaag gtgctaataa
      241 gggtttttag gttatcagga ctaccccatg tgctatattg gttattccat tgtcttggat
      301 ggtatcctac gccaccctct gataaagaag atgggggtaa ccaaaccata tcaaaattgg
      361 tagctatttc gctagccatt gtggtgagtt ttgtccagct tgtttccgat tgagagtccc
      421 aatagaaacc ctgtagcata acagcatcac tttggcttgg agcttgagct tgcagccaag
      481 ttaaattcat gacactcaaa actactaaaa tagtcatttt aagtttgttg attgttttca
      541 taaaaaatag tttaaggttt gtaattaata ctaaaacaat tggttatacc tttagcccaa
      601 ccttatggtg tgttaattag tgttaaatct taagataatt gtttttgctt ataaatgaag
      661 cagatgacta tttgttttat ttataatgtg agttggatag attacgtgga gtaaatatta
      721 aatattgtta tatatttttc atcaaggttg ggtttttctt actatgcttg ttcttaattt
      781 gttatatgtt atttaactta aatattcttt atttatatga aggctacttt actctaaatc
      841 caacgagtac atcttttgat aattcttttt tttgttacag ggggtatatc tgctgctgat
      901 ttaatcactt tgtccgttaa tgagaatcca attattactg gttttgtaga agatagccaa
      961 ttggcagggg caggtcaggt cgtatacaca aaaggaactt ctccgtatga atttttcttg
     1021 caagatcaga ataaatactc acatactggc tattttgacg gaatagaaat aggtaatcat
     1081 actgtgcagg tagtagatgc aaacatgtgt aaggctactt ttagttttta tcttgatatt
     1141 cccattatcc cagatttatt ttttactccc aatggcgatg gtgtaaatga tgtgtggaca
     1201 ataaaaaaca ttcaccttgt atctcattct gtttatattt atgatcgttt tggtaaatta
     1261 ctgtatactt acgaagataa ttttgatgga tgggatggta cttatcaagg aaatccatta
     1321 ccaaataccg attattggtt tttgattaac ataaagccta tcaataaaca gctgacaggt
     1381 cattttactt taaaaaggta gttgggttct aagtattatc agggactatt gccaacatag
     1441 tctctgataa ttatatcaac aaccgatata catcaggatt gtctttaaga agttgataac
     1501 ggattccttt tttattcatc ctgtcgataa gtccattata atcttctttt tgttgtatct
     1561 ctatacctac caatgcaggt ccatcttctc tgtttatttt ttttgtgtat tcaaatgtgg
     1621 tgatatcatc tgtagggctt agcacatcgt tcaaaaactc acgtaaggct cctgcacgtt
     1681 gcggaaaatt gataatgaaa taatgtttca gaccttcgta taagagtgaa cgttctttaa
     1741 tttcttccat tcgggtaatg tcgttattgc tccctgatag aatacatacg actgtttttc
     1801 cttttatttg ctcgcggtat tggtcgagta cggctatcga taatacgccc gatggctcta
     1861 ctacaatggc ttcttcgttg tagagtttta aaatacacga acacactttc ccttcgggaa
     1921 ccagagcaac ctctttgagg acttctttgc aaataggaaa attcaggtga ccaactttct
     1981 taaccgccgc tccatctaca aatttatcaa ttgtgccaag tgttaccaca ttatccgtgc
     2041 gtagcgattc gtacatagca ggagccccca aaggctctac gccaatgatt tgagtgtgtg
     2101 ggctcacttg tttaaaatag gctccaaccc ccgatgataa acctcctcct ccaacgggaa
     2161 taaacataaa atcaatttct tgatcagcat cttctagtat ttcaatagca atggtgcctt
     2221 gtccttctat aatttttttg ctgttgaaag gcggaatata taccatatta ttggctctgc
     2281 aaaatacatc tgcttcaaat tgtgcatcat caaatgtatc gccagttaga actatttcta
     2341 catattcttt tccaaacata cgtacttgac gcactttttg tttgggtgtg ggtgcaggca
     2401 taaagatggt tcctttaatg cgtaatttat tgcaagcata tgccaagcct tgagcatggt
     2461 tccccgcact tgcacatacc acgcctcttt ctaattcctc ctgcgataag cacgacattt
     2521 tattgtaggc accacgaatt ttgtaggaac gtacaatttg taaatcctcc cgtttcagat
     2581 aaatttgagc ttggtattct tccgacaaat tggcattgta ctccaaaggt gtacgtttta
     2641 ccacattttt tactcgttga taagctttcg aaatgtcagc tatttgtgga aaataagaat
     2701 ttcttgattc attcatactt ctgtagcgtt tgaattttgg gtgcaaatat agggattttg
     2761 tgttatgtaa ccaaaaacgc tctattaaca aagtaaatca ttacaaaatt catctttttg
     2821 tagatagttt gtaaggtttg gttgtgtaaa ataggcaggt tattagttat tggctgaatt
     2881 acataacgat tcggtagaaa gtgcctgttt atcggctatg cagcgatata gacacatttt
     2941 attttgtgaa tcggtatttt gtaatacaat ggtaatattt aacttaaaaa tcactaaata
     3001 gaaatgtctt tttgctaaaa taatatgttg tacaacataa atacagtaat tttgtggcat
     3061 caaattacca gcataattat atataatact ctaaatactt tacactatga attttaaaaa
     3121 cggaaaatgg aacaatgcgg ttgatgtaag agattttgtt tccttaaata ttactccata
     3181 cgatggagat agtggctttt tgtgttcacc tacacaacgt acactcgatt tgtggcaaat
     3241 atgtaaagat gccatttcag aagagcgtgc caataatggc gttcgctcca ttgacaatga
     3301 attagtgtct acaattactt cgcatgctcc agggtatatt gataaggaaa aagagttaat
     3361 tgtagggctt caaacagacc aactacttcg cagagctatg aaaccctatg gaggtattgc
     3421 cgtagtagaa aaagcatgta gcgaaaatgg tgttgaggtt agtccatttg taaaagaagt
     3481 atttactaaa tatgcaaaaa cgcacaatga tggcgtattt gatgtgtata cacctgaaat
     3541 tagaacattt cgttcgttag gtgtgcttac gggtttgccc gataattatg ctcgtggtcg
     3601 tataataggc gattatcgtc gtgtggcatt atatggtgtt aattttctta ttcaacaaaa
     3661 gaaaaaagat ttagaaagct tgctaggacc tatgagcgat gaaagaattc gcttgcgtga
     3721 agaggtttct gagcaaatca aagccctgta tgccattatc gaaatgggta aatcctatgg
     3781 atttgatttg tctcgtccag cacaaaatgc gatagaagct gtacaatggg tatatatggg
     3841 ttatttagct gccgtaaaag aacaagacgg tgcagctatg tcgttgggta atgtgtcgtc
     3901 gtttttagat atttacattg aacaagatat tgctgaagga aaaatttcag aagagtttgc
     3961 tcaagagctt atcgaccagt ttgtgatgaa attgcgtatg gtacgtcatt tgcgtatgga
     4021 tgcgtataac gaaatttttg ctggcgaccc aacttgggta actgagtcta ttgggggggc
     4081 attggctgac ggacgccaca aggtaaccaa aacatcatac cgtttcttac aaacattata
     4141 taacctcgga ccatcacccg agcctaatat gaccttgtta tggtcagaaa aattacccga
     4201 aaactttaaa aaattctgtg ctcaagtatc tattgacaca tcttctatac agtacgaaaa
     4261 tgatgatttg atgcaaagta cacgttgcag tgatgattat ggtattgctt gttgtgtatc
     4321 gtatcaagaa attggcaaac aaattcaatt ctttggtgct cgtaccaatt tagcaaaaac
     4381 attattgttg gctatcaacg aaggtcgttg cgaactttct ggtcaacaag tgctaacagg
     4441 tattccaaag ttgaagtcag aagtgcttga ttatgaggag gtaatggaag tgtttaaatt
     4501 taccatgaaa gaagtggcac gtgtgtatag cgaaacaatg aacattatcc actacatgca
     4561 cgataaatat tattacgaaa aagcacaaat ggctttgatt gataccaatc caaaaatcaa
     4621 tttagcctat ggtgtagctg gtttaagtat tgtagccgat tccttatctg ccatcaaaca
     4681 tgctaaagta acccctatac gtgatgaaca tggagtggca aaaagtttta aaattgaagg
     4741 agagtttcct ttttatggca atgatgacga ccgtgtagat gccattgctg tagaagtacc
     4801 tgcatatttc aatcaattat tgaaaaaatt gccaacgtat aaaaatgctg aacctacctt
     4861 gtctattctt accattacat ccaatgtagt gtatggtaaa aaaacaggag ctacgcctga
     4921 tggacgtgct catggggtgc cttttgctcc tggagctaat cctatgcatg gacgcgacca
     4981 tagtggagcg attgcctcac tcagttcggt atctaaatta gattattctg aggctcttga
     5041 tggtatatca aacaccttct ccattgtgcc aaaatcacta ggaccaacag acgaagaacg
     5101 tgcagataac ttagtgtcaa ttattgatgg ttattttaac aaagaagctc atcacttaaa
     5161 tgtgaatgtg ttgaatcgtg agatgctgga agatgctatg gagcatccag aaaattaccc
     5221 acagctaacc attcgtgttt cgggatatgc tgtaaatttt gttcgtttaa cacgtgcaca
     5281 acaacaagaa gtaatttcga gaagtttctt cgaatcactc taattttata aatacactac
     5341 atgattaatg tacattcggt tgagtcgtta ggtacgtacg atggacctgg tattcgcttg
     5401 gtgctttttt tacaaggatg taatttcaag tgcctctatt gtgctaatcc agacactatc
     5461 gagcctaaaa caggacgacc ttatggtgca gaagatatac tcaaaatggc tcgtaatcag
     5521 cgtcctttct tcggaaagaa aggcggggtt acggtgtcgg gaggtgaacc tttgctacaa
     5581 gctcgtgcat tgataccgat atttaaagcc ctcaaagaag atggttttaa cacatgcatc
     5641 gataccaatg ggagtgtgtt gactgatgcg gtaaaagaat tattgaccta taccgatttg
     5701 gtattgctcg atatcaaaca gatggatgaa gaagctcatc aggcaattac tggggttaaa
     5761 aatcatctta ctttgcgttt tgcgacttat ttggcagaaa ttggcaagcc tacatggatt
     5821 cgctatgtac ttgtgcctgg ttataccgac caacaagaac atttgcacca acttggagca
     5881 tttgtaaaag acatgaaaaa cgtagaaaaa atcgagatcc agccttatca caaacttggc
     5941 aaacacaaat acgaagcact tgggtggcaa taccagctcg aa
//
LOCUS       DAOPUR010000022         5699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_14087_length_5699_cov_2.969880, whole genome shotgun sequence.
ACCESSION   DAOPUR010000022 DAOPUR010000000
VERSION     DAOPUR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14087_length_5699_cov_2.969880"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKN90_00695"
     CDS             <1..282
                     /locus_tag="PKN90_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNZ84834.1"
                     /translation="KVKEEALGKTKLELCTEICHLLQTLPPHITEIKQLSVAINHPLC
                     PADNYDVVLDTVFNSVEDLQTYQVHLKHVEVAGFIKQVVFQRACIDVEC"
     gene            complement(332..2416)
                     /locus_tag="PKN90_00700"
     CDS             complement(332..2416)
                     /locus_tag="PKN90_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNZ84835.1"
                     /translation="MKPLRLFTISLLLLFTGYFVNSQNIGIQKNNPDFLEYLSSEEIQ
                     TGYVPLPFEMPTTPVGTKRSGESFPSQFDSRNVNGTSYILAPRNQVITGPCWAFAATD
                     VVQASFYKKGYQEGYLSPQLLTNCHNFVYTKTQGGNSMMAVATFARLVGPAFENAAPY
                     DANETACITTTKEDFPAFVSNVHMINNNIDAVKKAIMDNGSVATKMFYDVDYYNSADK
                     TYFYNGAANENHGISIVGWNDNMVVADKGYGTPSASGVWIEKNTWGTGFGDGGYFYVS
                     YEDTYVGKEVFAFTKRIDKNTIDKVYQYAQHGMTNSVGYTANNPSYGRIKFTTTEREK
                     IAAIGLYTYHPNTEITFWIYEGADFSKLRYTSEAEVIQYGGYYTIDITAPIYITGDFQ
                     VIVRYNHSTEAYAIPIEQNFPDYNNATIQNSGVQWISSSGSSWAPLGEDTSYKFNLCI
                     NAYTSIPNPPANDNVQDAVLLNTNTVYENFTNAGATVQANEVVPPYNNEEASCYTQNS
                     WCYEGGVQNSVWFKFVATNSVVNIITEGFDNQIALWKSNDDNAENLISGNTSTYQLLA
                     ANDDASVSNAFASLYNVQGLQVGKTYFIQVDGSANGAEGIFSIEIQDIPTSINSTTHA
                     STLLTPEGELTQEIKDKVNYIEAFDVSGRLLQILPKNSSLSNSKLPKQQVLIIKLHYT
                     DGGSDTISWLQN"
     gene            complement(2428..4503)
                     /locus_tag="PKN90_00705"
     CDS             complement(2428..4503)
                     /locus_tag="PKN90_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNZ84836.1"
                     /translation="MGANEGLSKKAKIFNEIIKDLQYILAIGIIVVLLPSDLQYKYQY
                     TEGKPWQYDLLTATFDFPIYKTEVEISNQRNDIIKTHKPYYVLDTSLKSKQIAFLELI
                     VQSLFAKDTISPVSTHVISELKKSLINLYDTGILTSQDQSALLAKEAKYIVIMQNNLG
                     QTRRVEDIITEKEAIRLFLKDASRILPESTLNKLPINELIIGNLVYDAETTKKIKNDE
                     LNNLSLTSGQIQAGERIIDRGEIVTYEKQKILDSLKKQFHQEERKSNNNTILLGEILI
                     VSCLILLFYLYARLFRKKFFISKRFVSVTLLFIIGFTVATSMIVQHANPKWVYIIPYS
                     LLPIVLSTFFDTRTGLFAHVITVLLSSFMVPLPFEFVLLQITTGMVAIYSLKDLAQRS
                     QLVQSAIIIVISYCLIYFGYGITIDGDITRINWTMFIYFFVNGVLLLFAYPLIYILEK
                     MFGYISNVTLIELANTNNKVLRNLSELAPGTFQHSMQVSNLASEIAIKVGANPLLART
                     GAMYHDIGKMKNPAFFTENQNKGNNPHQQLSFEDSARIIIQHVEDGVQIAKDANIPNC
                     IIEFIQTHHGTNKVRFFYNSYRLQFPEKEIDDSIFTYPGPNPSTKETAIVMLCDAVEA
                     ASRSLPEYTDEAIDGLVEKIITSLLQEGSLNEAPLTMQHISLTKLILKEKLKNIYHTR
                     ISYPELQKK"
     gene            4722..5033
                     /locus_tag="PKN90_00710"
     CDS             4722..5033
                     /locus_tag="PKN90_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNZ84837.1"
                     /translation="MENSNNGFSIEIADDIAEGVYSNLAIIAHSTSEFVIDFARLMPN
                     TPKAKVKSRIILNAEHAKRLLLALQENITKFEKQNGKIILSEQKNPPIPMNFGGMKGE
                     A"
     gene            5191..5607
                     /gene="rpsL"
                     /locus_tag="PKN90_00715"
     CDS             5191..5607
                     /gene="rpsL"
                     /locus_tag="PKN90_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNZ84838.1"
                     /translation="MPTIQQLVRKGRAKQIDTSKSPALDSCPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNQKEVNSYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGTL
                     DTAGVNGRKQKRSKYGAKRPKPGQPAAAPAKGGKKK"
BASE COUNT         1828 a         1003 c          956 g         1912 t
ORIGIN      
        1 aaggtgaaag aagaagcttt aggcaaaacc aagctggagt tatgcaccga aatttgccac
       61 ttattacaaa ccttgcctcc acacattacc gaaatcaagc aattatctgt tgccatcaat
      121 catcctttat gtccagcgga caattacgat gtagtattag atacagtatt taattctgta
      181 gaggacttgc aaacctatca agtgcatctc aagcacgtag aagtggctgg ttttatcaaa
      241 caagtagttt ttcaaagggc atgtattgat gtcgaatgct aaaacaaaaa atcctttgac
      301 ttgcatcaaa ggattttttt tagtcaattc gttagttttg caaccaacta atggtatcgc
      361 taccaccatc agtataatgc aacttgatga ttaacacttg ctgtttaggt aatttgctgt
      421 tgctcaaact actatttttt ggcaatatct gtagtaatcg tccacttaca tcaaacgcct
      481 cgatatagtt caccttatct tttatttctt gtgttaactc gccttcaggt gttaataaag
      541 tagatgcatg ggtggtgcta ttaatagaag ttggaatgtc ttgtatttca attgagaaaa
      601 taccctctgc accattggca gaaccatcta cttggataaa ataagttttc cctacttgca
      661 atccttgcac attatacaat gaagcaaagg cattgcttac actggcatca tcgttggctg
      721 ctagtagctg ataggtagat gtgttgccac tgataaggtt ttccgcatta tcatcatttg
      781 atttccaaag ggctatctga ttatcaaagc cttctgtaat tatatttact acagagttag
      841 ttgctacaaa tttaaaccat accgaatttt gtaccccccc ctcgtaacac caactgtttt
      901 gtgtataaca acttgcttct tcgttattgt atggtggcac aacttcatta gcttgtactg
      961 tagctccagc gtttgtgaag ttttcgtaca ctgtatttgt gtttagcaat acagcatctt
     1021 gcacattatc gttagcagga ggatttggta tagaggtata tgcattaata cacagattaa
     1081 atttataaga tgtatcttca cctagaggag cccaagaact gccagaagag ctgatccact
     1141 gtactcccga gttttgtatt gtagcattat tgtagtcagg aaaattttgt tctatgggta
     1201 ttgcataagc ttccgttgag tgattgtagc gtactattac ctgaaaatct ccagttatat
     1261 atattggtgc agtaatatct attgtataat atcctccata ttgaattact tctgcttcgg
     1321 atgtatagcg taatttgcta aaatcagctc cttcgtaaat ccaaaatgta atctcggtat
     1381 tggggtggta tgtatacaaa cctatggctg ctattttttc tcgttcagtg gtagtaaatt
     1441 taatgcgtcc gtatgatggg ttattggcag tatagcctac actatttgtc atcccatgct
     1501 gtgcatattg atataccttg tcaatggtat ttttatcaat acgcttggta aatgcaaata
     1561 cctctttccc aacatatgta tcttcgtacg atacataaaa ataaccccca tctccaaaac
     1621 cagtacccca agtgtttttt tctatccaaa cacccgaagc tgatggagtt ccatatcctt
     1681 tgtcggcaac caccatatta tcgttccaac ccacaatgct aataccatga ttttcgttgg
     1741 ctgctccatt ataaaaatac gttttatcag cactattata ataatctaca tcgtaaaaca
     1801 tttttgttgc aaccgatcca ttgtccatga ttgctttttt taccgcatca atattattat
     1861 ttatcatatg tacgttcgaa acaaatgctg gaaagtcttc ttttgttgtt gttatacagg
     1921 ctgtttcatt cgcatcatat ggtgccgcat tctcaaaagc aggaccaact aaacgggcaa
     1981 atgtagctac tgccatcatc gaatttcctc cttgtgtttt tgtatacacg aagttatgac
     2041 agtttgttaa tagttgtggt gataaatatc cttcttgata tcctttttta taaaaagaag
     2101 cttgtactac atctgtagct gcaaaagccc aacaaggacc agttattacc tgatttcttg
     2161 gtgctaaaat ataactagtt ccattcacat tgcgagagtc gaattgtgaa gggaaacttt
     2221 ctccgctcct ctttgttcca acaggtgttg tgggcatttc gaagggtaaa ggaacataac
     2281 ccgtttgtat ctcttctgat gaaagatatt ccaaaaaatc agggttattt ttttgaatac
     2341 caatattttg tgaatttaca aaatagccag taaacagtaa taacaaactt atagtaaaca
     2401 gtctaagtgg tttcattggg ttttaattta ttttttttgt aattcaggat aagatattct
     2461 agtgtggtaa atgttcttta gtttctcttt taatatgagt tttgtcaatg atatatgttg
     2521 cattgttaag ggtgcttcat ttaacgagcc ttcttgtaat aatgatgtga taattttttc
     2581 tactaaacca tcaatagctt cgtctgtata ttcgggcaaa ctacgtgaag ctgcctctac
     2641 tgcatcacat aacataacaa tagctgtttc tttcgtactt gggtttggtc cagggtatgt
     2701 aaatatacta tcgtcaattt ctttctcagg gaattgcaaa cggtacgaat tgtaaaaaaa
     2761 tcgaactttg tttgtgccgt gatgtgtttg gataaattca attatacaat tggggatgtt
     2821 tgcatctttg gctatttgaa caccatcttc aacatgttgg ataataatac gagcactatc
     2881 ttcaaaactc aactgttgat gaggattatt tcctttgttc tgattttctg taaagaaagc
     2941 cggatttttc attttcccaa tgtcgtgata catggctcct gtgcgagcaa gtaatggatt
     3001 agctcctacc ttaattgcaa tctctgaagc caaattcgac acttgcatcg aatgctggaa
     3061 agtaccaggg gctagttccg ataaattacg gagtacctta ttatttgtat tggctaattc
     3121 aattaatgtt acgttagaaa tatatccaaa cattttttct aaaatataaa tgagaggata
     3181 agcaaataat aaaaggactc catttacaaa gaaataaatg aacatagtcc aattaatgcg
     3241 agtaatatct ccatcaattg taatgccata tccaaaataa attaagcagt agctaataac
     3301 aataatgata gctgattgta ctagctggga gcgttgagcc aaatctttta ggctgtagat
     3361 tgcaaccatt cctgtcgtta tttgtaataa aacaaattca aatggcaatg gaaccataaa
     3421 tgaagagagc aatacagtta taacatgtgc aaataatcct gtacgggtat caaaaaaggt
     3481 gcttagcacg ataggcagca acgaataagg gataatatat acccatttag gattagcatg
     3541 ttgcacaatc atcgaagttg ctacagtgaa cccaattatg aataataacg taacactaac
     3601 aaaacgtttg ctgataaaaa acttcttcct aaataaacga gcataaaggt aaaacaataa
     3661 aattaaacag cttacaatca aaatctctcc taaaagaatt gtattattat tcgattttct
     3721 ctcttcttgg tgaaattgtt ttttcaagga gtctaagatc ttttgtttct cataagttac
     3781 aatttccccc ctatcaataa tacgttctcc agcttgtatt tggcccgaag tgagcgataa
     3841 attattcaat tcatcatttt ttattttttt tgtcgtttca gcatcataca ccaaattgcc
     3901 tattattaat tcattaatag gtagtttatt aagtgtagac tctggaagta tacgacttgc
     3961 atcttttaaa aacaatctga tagcctcttt ttctgtgata atgtcttcta ccctccgtgt
     4021 ttgacctaga ttattttgca taatcacaat gtatttagct tctttagcaa gtaatgcaga
     4081 ttgatcttgt gatgttaaaa ttccagtatc atataagttt attaacgatt tttttagttc
     4141 agaaattaca tgggtagaaa ctggcgaaat tgtgtctttt gcgaataacg attgtactat
     4201 taattctaag aatgcaattt gcttgctttt aaggcttgta tctagtacat aataaggttt
     4261 gtgcgttttt attatatcat tacgctgatt actgatttca acttctgttt tataaattgg
     4321 gaaatcaaat gtagccgtaa gtaaatcata ctgccaaggc ttgccttcag tatattgata
     4381 tttgtattgc aaatcgctgg gaagtaatac tacaataata cctatcgcca aaatatactg
     4441 gagatcctta ataatttcat taaatatttt tgccttttta ctcagacctt catttgctcc
     4501 cataacgctc tcttcttttc agagaaaata ctacaaaatt aacaagcaaa tgtaaatatt
     4561 ttaattgaga attcaatgat ttaatttaaa aatcgtactt ttgtctgtta attaacatga
     4621 aaccatacaa aatgtgtcaa aattgataca aaatagtaga aacataaggt ttttttagaa
     4681 aaaacatctt tcttaattac ataattttaa aacaaaatag aatggaaaat tcaaacaatg
     4741 gttttagtat agaaattgca gacgacattg cagaaggtgt gtattcaaat ttagcaatta
     4801 tagcacattc aaccagcgaa tttgtaatag actttgctcg attaatgccc aataccccca
     4861 aagctaaagt gaaatctcgc attatcttaa atgccgagca tgctaaaaga cttttactag
     4921 cattacaaga aaatattact aaatttgaaa aacaaaacgg gaaaataatc ctttcagaac
     4981 aaaaaaatcc ccctattccc atgaattttg gtgggatgaa aggagaagca tagttcgtat
     5041 gcttctatta aataattgtt tctcaaataa atttgtattt ttttatacta aaatattgtt
     5101 tagtacaaaa gtttgtgtat ctttgcaacc cgaatttaca aaaaatgggt tcaaaaagaa
     5161 gtctaataat ttataacaaa aacaagtaaa atgcctacaa ttcagcaatt agtaagaaaa
     5221 ggaagggcta aacagatcga cacaagcaag tctccagcgt tggactcttg tcctcaacgt
     5281 cgtggtgtgt gcgtaagggt gtatacaaca actcccaaaa aacctaactc ggctttacgt
     5341 aaagtagcaa gagttcgtct aactaaccaa aaggaagtaa actcatatat tccaggtgaa
     5401 ggacacaact tgcaagaaca ctcaattgtg ctaattcgtg gtggtcgtgt taaagattta
     5461 ccaggggttc gttatcacat tgtacgtgga acattagata ctgctggcgt taatggtcgc
     5521 aaacaaaaac gttctaaata cggagctaaa cgcccaaaac caggtcaacc agctgctgca
     5581 ccagctaaag gaggtaaaaa gaaataatcc ttcggtgtga aaattaaaag aaaaacaaac
     5641 aacagttaat tactactgtt ttgcatattt tggatttaga cattttatcg gttgagtaa
//
LOCUS       DAOPUR010000023         5671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_14200_length_5671_cov_3.408120, whole genome shotgun sequence.
ACCESSION   DAOPUR010000023 DAOPUR010000000
VERSION     DAOPUR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14200_length_5671_cov_3.408120"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKN90_00720"
     CDS             <1..602
                     /locus_tag="PKN90_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNZ84839.1"
                     /translation="TEKEKLILPEYGRNIQQMVNHALTIEDKAERTRCVHSIIAIMGK
                     LFPHLRDVNDFKHKLWDHLAIMSNFTLDIDYPYEVPKQEILNTKPERIPYPSIHIKYR
                     HYGKTVDTMVKKAIEYPEGDDKKRFIELIANYMKKSFLTWNKEGVDDEKIYEDLKELS
                     NGQLIYTSESLKLKESKEILAQNKVNKSKQNIPHKKIKK"
     gene            607..1914
                     /gene="murA"
                     /locus_tag="PKN90_00725"
     CDS             607..1914
                     /gene="murA"
                     /locus_tag="PKN90_00725"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNZ84840.1"
                     /translation="MASFVIEGGHSLSGEITPQGAKNEALQVICATLLTKQKVTIHNI
                     PSILDVNNLIDLLKNIGVRVEKISKHTYSFQADAIDIDFFTSTAYYKQSSALRGSVML
                     VGPLVARFGYAIIPKPGGDKIGRRRLDTHFVGIQKLGAEFTYDPTHQLYKIEAKKLQG
                     TYMLLEEASVTGTANILMAAVLAQGKTTIYNAACEPYLQQLCKMLNAMGAKISGIGSN
                     LLTIEGVESLGGCEHTILPDMIEIGSFIGMAAMTNSSITIKNVSYENLGIIPDSFRKL
                     GINIEVSGDDIIVPKQSGFTIESYIDGSIMTLADAPWPGLTPDLLSVLLVVATKAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATIIGLGENFRLRKAVMSSPDIRAGI
                     ALLIAAMSAEGTSVIHNIEQIDRGYEDIELRINQLGGKIKREA"
     gene            complement(1950..2450)
                     /gene="tpx"
                     /locus_tag="PKN90_00730"
     CDS             complement(1950..2450)
                     /gene="tpx"
                     /locus_tag="PKN90_00730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNZ84841.1"
                     /translation="MAKILFKGGEVNTNGDLPKVGSVAPNFTLVNTGLADVSLADYKG
                     KNVVLSINPSLDTSVCAATARKFNTTLSQLANTVVLYITKDLPFAHNRFCTTEGIENV
                     VTLSDYRNPEFATTYGVLVQDGALKGLMARAIVVINPQGVVTYTEMVPEITTEPNYDN
                     VIAALK"
     gene            2641..3771
                     /locus_tag="PKN90_00735"
     CDS             2641..3771
                     /locus_tag="PKN90_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84842.1"
                     /translation="MVIILFSSCEEWNIKTYNVPSEFQPYVDKFKTDAKKYGYNFDDK
                     GLIVRFADLDNNIAGLAYYKRNPILIEIDREYWASASNTKNAHDIKENLLFHELGHGF
                     LQRMHDNTVLANGDWKTMMCGDKLPNDRASNINYRGFRKAYYIEELFTRTNDTPAWST
                     LIPQFDNIDENVILQQDFSSGSDWTIGSNSLYESSIENGAYTFTTKTSQAFYVLNKGT
                     LNTSNDFYIEVRLKASAGLDDSFGLVCGSFNDGNTPTSLHYFYQKGNNHMYIGESECL
                     GPFIDLYTEILHPNEFNTFAIRKYNNMLYYYINDTFIYHNDLDEIIIMYGSQIGFKIP
                     GNSTLYVDYAEVRENSTGLKKRNTTELSVEKATEKKVIHWNK"
     gene            complement(3776..4375)
                     /locus_tag="PKN90_00740"
     CDS             complement(3776..4375)
                     /locus_tag="PKN90_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ84843.1"
                     /translation="MSRTRVIHTTITSTRELLLEVARQLFAKVGFTNTTMNDIAKAAK
                     RGRRTLYVYFKSKEDVYYAVVEQKMYKLLDALELVANSAEPPQNKLIDYIYTRLDKTR
                     EVVKNNGTLRADFFSDYKTVEMVRKKMEIKEHELLKKILKEGVDAGIFYVRSIDFAAF
                     LVLNAIRGLELPYMKQSVADRINDSREDIVRVLFHGLLK"
     gene            4500..>5671
                     /gene="tilS"
                     /locus_tag="PKN90_00745"
     CDS             4500..>5671
                     /gene="tilS"
                     /locus_tag="PKN90_00745"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNZ84844.1"
                     /translation="MIHLVTHYIHTHKLLSPAATVIVGVSGGADSMALLYILKEIGYN
                     CVVAHCNFTLRQKESDADESFVVDYCRTNNLPCLVKHFDTKKHAQDNKISIEMAAREL
                     RYGWFETLRLHYQAEAIAIAHHFNDSIETILINLINGTGINGLTGIKPINGHIIRPLL
                     CVNRKEIEDFLQKKHLHFITDSSNVENIYTRNKIRNILIPQIEEINPSFINTMQGTIH
                     NLTTSATIYNSYIELKLKDIIESSPNNQVKISINQLLKEEHADTVLYEALKTYNFSPK
                     LCASILNNVANSGKEYLSPTHKLLIDRDFIFLTPIKEKNDTHTYYIQQSDSSIEFPIK
                     LNINILRNDASFHLSKKSTCACIDYSLLKFHLTIRRWKKGERFYPLGMKQSKKVSD"
BASE COUNT         1997 a         1021 c          946 g         1707 t
ORIGIN      
        1 acacagagaa agagaaatta atcttgcccg aatatggtcg taatattcaa caaatggtca
       61 atcacgcatt aacgattgag gacaaagccg aacgcacacg ctgtgtacat agcatcattg
      121 ccattatggg taaactgttt cctcatcttc gtgatgtaaa tgattttaaa cacaaacttt
      181 gggatcattt ggctattatg tcgaacttta cattggatat tgactatcca tacgaagtgc
      241 caaaacaaga aatactgaat acaaaaccag agcgaattcc gtatcctagc atacatataa
      301 aataccgcca ttacggtaaa accgtagata ctatggtaaa aaaagccatt gaatatcctg
      361 aaggcgatga caaaaaacgt tttattgagc tgattgccaa ctacatgaaa aaaagtttct
      421 taacatggaa caaagaaggg gtagatgacg agaaaatata cgaagatttg aaagaactct
      481 ctaatgggca attaatctat acgagtgaat cgctcaaact caaagaatcc aaagagatac
      541 tggctcaaaa caaagtgaat aaaagcaaac aaaacatacc gcataaaaaa attaaaaagt
      601 aagtctatgg catcttttgt aatagaagga ggacattctt tgtcgggaga aataacaccg
      661 caaggagcta agaatgaagc actacaagtg atatgtgcca cattgctaac aaaacaaaaa
      721 gttaccattc ataatatccc tagtattttg gatgtaaaca acttaataga tttactaaaa
      781 aacattggtg tacgtgttga aaaaataagt aagcatacct actcttttca ggctgacgcg
      841 attgatattg atttttttac gagtacagcc tattacaaac aaagcagtgc acttagaggc
      901 tcagtaatgt tggtaggtcc actagtggct cgttttggtt atgccataat acccaaacca
      961 ggtggcgata aaattggtcg taggcggttg gatacacatt ttgttggcat acaaaaattg
     1021 ggagccgagt ttacgtacga ccctactcat caactctaca aaattgaagc caaaaagcta
     1081 caaggaacat acatgttatt agaagaagct tcggtaacag gtactgctaa cattctaatg
     1141 gctgccgtat tagctcaagg caaaacgact atctacaatg cggcatgcga accgtatttg
     1201 caacaattat gtaagatgct aaatgccatg ggagcaaaaa taagtggtat tggctctaac
     1261 ttgcttacaa ttgagggcgt tgaatcattg ggtggttgcg aacataccat attacccgat
     1321 atgattgaaa ttggtagttt tatcggtatg gcagctatga ccaactcttc tatcacaatt
     1381 aaaaatgtat catacgaaaa cctgggtatc atccctgata gttttcgcaa attaggcata
     1441 aacatcgaag taagcggtga tgatattatc gtgccaaagc aatcgggatt tacaatcgaa
     1501 tcgtatatcg atggctctat catgacctta gccgatgctc cttggcccgg attaacaccc
     1561 gacttgctta gtgtattatt ggtagtagcc accaaagcca aagggagtgt tttgatacat
     1621 caaaaaatgt tcgaaagtcg cctatttttt gtagataaac tcattgacat gggtgctcaa
     1681 atcatcttat gcgatccgca tagagctaca attataggat tgggagaaaa tttcaggtta
     1741 cgcaaggcgg taatgagttc gcctgatatt cgtgctggca tagccttgct aatagcagca
     1801 atgagtgccg aaggaacaag cgtaatacac aatattgaac aaatagacag gggatacgag
     1861 gatatagaat tacgcatcaa ccagttaggt ggtaaaataa aaagagaagc ataaagcttc
     1921 tctttttatt ttcagacaat tgagtgctgt tattttaaag cagcaattac attgtcgtaa
     1981 ttaggttcgg tggtaatttc tggaaccatc tctgtatacg taaccactcc ttgtggatta
     2041 ataactacga tagcacgtgc cattaaacct tttaaggctc catcttgtac taacacgcca
     2101 tacgttgtag caaactcagg attacgataa tcggataaag taaccacatt ctcaatgcct
     2161 tctgttgtac aaaaacgatt gtgtgcaaaa ggtaaatctt tggtgatgta caacaccact
     2221 gtattcgcta attgcgacaa ggtggtgtta aatttgcgtg cagtagctgc acatacagac
     2281 gtatccaaac tgggattgat actcaaaact acattctttc ctttgtaatc tgctaacgaa
     2341 acatctgcta agcctgtatt cactaaggta aaattaggtg ctactgaacc tactttaggt
     2401 aaatctccat tggtatttac ctctccgcct ttaaaaagta tttttgccat ataatatagt
     2461 tatttaaatt aaacaaacgc aatgtacgtc ttttttctaa tagaaattct aaaatcaaag
     2521 tattttttgc ttttttttaa taaaaaagag aatatttgac agttaataca taaaaaaaga
     2581 acttacattt gtaaattact ttaagctaaa aaaataatgc gtaatgcttt ctttttcgtt
     2641 ttggtcataa tactcttttc ttcatgtgaa gaatggaata taaaaaccta taacgttccg
     2701 tctgaatttc aaccttatgt agataagttt aaaacagacg ccaaaaaata tggatataat
     2761 tttgatgaca aaggactaat tgtacgtttt gctgatttag acaacaatat tgcaggctta
     2821 gcttattaca aaagaaatcc tattttaatt gaaattgata gagaatactg ggcatcggct
     2881 agtaatacaa agaatgcaca tgatattaaa gaaaacctac tgtttcatga attagggcat
     2941 ggatttttgc aacgtatgca cgataatact gtattagcaa atggagattg gaaaacaatg
     3001 atgtgtggcg ataaactccc aaacgacaga gcctctaaca tcaattatag aggttttaga
     3061 aaagcatact atatcgaaga actatttact cgcaccaacg atacgccagc atggagtaca
     3121 ctcatccctc aatttgataa tatcgacgaa aacgtcattc tacaacaaga tttttctagt
     3181 ggttcagatt ggacaatagg ctccaatagt ttatacgaaa gttccattga aaatggagca
     3241 tatacattta ccaccaaaac ttctcaggct ttttatgtat taaacaaagg tactctgaat
     3301 acaagcaatg atttttacat agaggttcga ctcaaagcat cagcaggtct tgatgactca
     3361 tttggacttg tttgtggctc atttaacgat gggaatacgc caacttctct tcattatttt
     3421 taccaaaaag gaaataatca tatgtatata ggagagtccg aatgtttagg tccatttatt
     3481 gatttatata cagaaatttt acatcctaac gaattcaaca catttgctat acgtaaatac
     3541 aataatatgt tgtactatta tatcaacgac acctttatct atcacaacga tttagatgag
     3601 attattatca tgtatggctc acaaattggg tttaaaattc ctggcaatag caccctatat
     3661 gttgattatg cagaggtcag agaaaatagc actgggttga aaaaacgaaa tactaccgaa
     3721 cttagtgtgg aaaaagcaac ggagaaaaaa gtcattcatt ggaataagta acaggctact
     3781 tcaataaccc atgaaacaac acacgaacga tatcttccct gctatcgttt attctatcag
     3841 ctacagactg tttcatatag ggtagctcta atcctctaat tgcattcaat actaaaaaag
     3901 cagcaaaatc aatacttcta acataaaaga ttccagcatc aacaccttct ttaagaattt
     3961 tctttagtaa ttcatgttct ttgatttcca ttttttttcg taccatttcc accgttttat
     4021 aatcactaaa aaaatcagct ctcagtgtac cgttattttt aacaacctct ctggttttat
     4081 ctaatcgtgt atagatataa tcaatcaatt tattttgagg aggttcggca gaatttgcta
     4141 ctaactccaa agcatccagc aatttataca ttttctgttc tactactgca taataaacat
     4201 cttccttgct tttaaaataa acgtataagg ttcgccttcc ccgtttagcc gctttagcaa
     4261 tatcattcat agtagtattc gtaaaaccaa ctttagcaaa caattgcctg gcaacctcta
     4321 acaataactc cctagttgac gtaatggttg tatgtataac tctagttcta ctcattgcac
     4381 aataatgtta ctttgtgcaa aaataacaga gttatttcaa tttatcaaat aattaagcat
     4441 tttttttatt attcctccaa aaacgtaaaa aatatatcgt acttttgcat atatagcata
     4501 tgatacatct agtaacacac tacatacata cacacaaatt actctctcct gcagctactg
     4561 ttatagttgg ggtaagtgga ggagccgatt ctatggcatt actctatatt ctcaaagaaa
     4621 tcggatacaa ttgtgtggtg gctcattgta attttacatt acgccaaaag gagtctgatg
     4681 cagatgaaag ttttgttgtt gattattgtc gcaccaataa tttaccctgt ttagtcaaac
     4741 actttgacac aaaaaaacat gctcaagaca ataaaatttc tattgaaatg gcagcgagag
     4801 aacttcgcta cggctggttt gaaactcttc gattacacta tcaagccgaa gccattgcaa
     4861 tagctcatca cttcaatgat tctattgaaa ccattctcat aaacttaatc aatggaacag
     4921 gtataaatgg attaacaggt atcaagccca taaatggaca tattattcgt cctttattat
     4981 gcgttaatag aaaagagatt gaagattttt tacagaaaaa acacttgcat tttattactg
     5041 actcaagtaa tgtagaaaat atatatactc gcaataaaat aagaaatatt cttattcccc
     5101 aaatagaaga gataaaccct tcttttataa acacaatgca gggcactatc cacaatttaa
     5161 caacatctgc tactatttat aatagctaca ttgagttgaa gctaaaagat attattgaat
     5221 caagcccaaa taatcaagtt aaaatctcta taaatcagtt actaaaagaa gaacatgcag
     5281 acactgtgct atatgaagct ctcaaaacgt ataatttttc acctaaactg tgtgcatcaa
     5341 tactcaataa tgtagcaaat agcggaaaag agtacctgtc accaacacat aaactcctca
     5401 tagacagaga ttttattttt ttaactccca ttaaagaaaa gaatgataca catacctatt
     5461 atatccaaca gtctgattca tcaatagaat ttcctatcaa actgaatata aacatcttac
     5521 gaaacgatgc gagttttcat ttatccaaaa agtcaacctg tgcttgcatt gattattctt
     5581 tgctaaaatt tcatcttaca attaggagat ggaaaaaagg ggagcgattt tatcctttag
     5641 gaatgaaaca aagtaaaaaa gtaagcgatt t
//
LOCUS       DAOPUR010000024         5657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_14255_length_5657_cov_3.074259, whole genome shotgun sequence.
ACCESSION   DAOPUR010000024 DAOPUR010000000
VERSION     DAOPUR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14255_length_5657_cov_3.074259"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2349)
                     /locus_tag="PKN90_00750"
     CDS             complement(<1..2349)
                     /locus_tag="PKN90_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNZ84845.1"
                     /translation="MKSFKTMNTLFGWISFLIAWVVYALTIEPTASFWDCGEFITSAF
                     KLQVGHPPGAPFFNLTGNFFTLFAKDPSQVAIMVNLMSATLSAFTILFLFWTITHIAK
                     KIIAQNGSEITLSQSITIIGSGLIGSLVYAFSDTFWFSATEAEVYAYSSFFTAIVFLL
                     ILKWEDQHDKAGSDKYLILIAYLMGLSIGVHLLNLLTIPALVLVYYFRKTEKVSLKGG
                     TWALLISFAILGFIMYGFIPQFIANAGIAELFFVNTLGFSFNTGAFFYYFTTIAVLVW
                     AIIELYSKKPSATRIKVSFIGSLILLGIPFLLGSLLLGLVLLSILIFYFIKLKEKISI
                     RLLSTIIISLTMMLIGYSSYALIVIRSAANPPMDQNSPDNIFALQSYLNREQYGDRPL
                     FYGAYYSAEPKLIEEDGYCKPSVKEGAKTWGQAEKTSPNEKDRYIVTGTKFELEYDSQ
                     FKTIFPRLHSSNESHIKAYNSWVTIQGKTVKYDKCGRIIDVVVPTFGENLRFFFSYQL
                     NYMYWRYFLWNFSGRQNDLQGSGEVTRGNWITGFNFIDNYLVGDQTNLPPDLAENKAH
                     NAYFMLPLLLGIIGIIYQLMWSNKKGNQAFWVIGWLFFMTGIAIVMYLNQGPSEPRER
                     DYAYAGSFYAFSIWIGFGVAGIAKWLESKKINPTLAAIIALLLAAPAPIIMAQQNWDD
                     HDRSNRYVVRDFAQNYFNSLAPNAIIFTNGDNDTFPLWYTQEVEGTAVSLDKRVCNLS
                     YLNTDWYIDQMKRPAYASAPLPISWGRETYGEGKRNYACAYNL"
     gene            2527..2796
                     /gene="rpsO"
                     /locus_tag="PKN90_00755"
     CDS             2527..2796
                     /gene="rpsO"
                     /locus_tag="PKN90_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNZ84846.1"
                     /translation="MQLTKEQKQEIFEKYGKSNTDTGSSEGQIALFSARIAHLTEHMK
                     SNHKDHSTERALVMLVGKRRRLLDYLKAKDIERYRAIIKELGIRK"
     gene            3071..5656
                     /gene="mutS"
                     /locus_tag="PKN90_00760"
     CDS             3071..5656
                     /gene="mutS"
                     /locus_tag="PKN90_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ84847.1"
                     /translation="MKQYNQVKAKHPDAVLLFRVGDFYETFSDDAIRASEILGITLTR
                     RANGAASYVELAGFPHHALDTYLPKLVRAGLRVAICEQLEDPKTAKGIVKRGITELVT
                     PGVSYNETTLNFKENTFVAAVHFEKSRVGVAFLDISTGEFLTAEGSSEYVDKLLTSFS
                     PKEVLYERGKTSLFEQSFGTHFLSFTLEDWVFVSDTANERLQKQFQTKNLKGFGVVDL
                     PLGIIASGAILHYLEFTNHLQTAHITHLRRIEEERFVWLDRFTIRNLELYQAMHDGGK
                     SLLDVLDKTLTPMGARMLKRWLAFPLKNKAAIEHRLSIVSYFFKDKDIQQVLYQQLSL
                     IGDLERLVSKIAVGRINPRELVQLKIALTAVETIKNSSTLVDNEALVSIAERLQSCTR
                     MRDKIEQTIASDAPMLLHKGGVIKQGIHPELDEYRRLAYSGKDYLLQIQQRESEVTNI
                     PSLKISYNNVFGYYIEVRNTHKDKVPSNWIRKQTLVSAERYITEELKEYEEKIVGAEE
                     KIAQIEGQLFNELVVSLCDYISALQLNASLIAELDCLLSFANVSANNSYIPPVITETD
                     VLSIKQGRHPVIEKQLPMGESYIANDVYLDTNTQQIMMITGPNMAGKSALLRQTALIV
                     LMAQIGCFIPAESAEIGIVDKIFTRVGASDNISMGESTFMVEMSEASSILNNVSPKSL
                     ILFDELGRGTSTYDGISIAWAIVEYLHEYPQAKAKTLFATHYHELNEMEKTFSRIKNF
                     NVAVKEMDNKIIFMRKLVPGGTEHSFGIHVAKMAGMPKSVVKRSNEILAQLEKDNRKT
                     GQPQKHIDTVAHQREGLQMSFFQLDDPVLIQIRDEIKNVDVNNLTPLQALNKLNEIKK
                     IITGK"
BASE COUNT         1893 a          935 c         1077 g         1752 t
ORIGIN      
        1 cagattatac gcacatgcat agttgcgttt tccttctcca tatgtttctc ttccccaaga
       61 aataggtaat ggagctgatg catacgcagg acgtttcatt tggtcaatat accaatctgt
      121 gtttaaatag cttaaattac atacccgttt atcaagagaa acggctgttc cttctacttc
      181 ttgagtgtac cataaaggga atgtgtcgtt atctccattc gtaaaaatga tagcattggg
      241 agctagcgaa ttaaaatagt tttgagcaaa atcgcgtact acatatctat ttgaacggtc
      301 atggtcatcc caattttgtt gagccataat aataggagca ggagcagcta ataaaagggc
      361 tataatggct gctaatgtag ggtttatctt ttttgattca agccatttgg ctataccagc
      421 aacgccaaaa cctatccaaa ttgagaatgc ataaaacgat ccagcatagg catagtcgcg
      481 ttctcttggt tctgatggac cttggttcag gtacatgaca atagcgatac ctgtcataaa
      541 gaaaagccaa ccaataaccc aaaaggcttg attccctttt ttgtttgacc acattagttg
      601 atatattatc ccaattattc ctagtagtaa aggaagcatg aaataggcgt tatgagcttt
      661 attttctgcc aaatctggtg gtagatttgt ttggtctccc accagatagt tatcaataaa
      721 attaaatcca gttatccaat tgcctcgagt aacctctcca ctgccttgta aatcattttg
      781 acgtccagaa aaattccata agaaataacg ccaatacata taattgagct ggtaagagaa
      841 aaagaaacga aggttttcac caaatgtagg cactactaca tcaatgattc tgccacattt
      901 atcatatttt acggttttcc cctgtatagt aacccaacta ttatatgctt ttatatgact
      961 ttcattagag ctgtgtagac gaggaaaaat tgttttaaat tgactatcgt attccaattc
     1021 aaatttggtt cctgtaacga tatacctgtc cttttcatta ggggatgttt tttctgcttg
     1081 accccatgtt tttgcacctt cttttacaga tggtttacag tagccatctt cttcgattaa
     1141 cttaggttcg gcagagtaat atgctccgta aaataatggt cgatcaccat attgttcgcg
     1201 atttagatag ctttgtaacg caaaaatatt gtcgggtgag ttttggtcca tgggaggatt
     1261 ggcggctgaa cgaataacaa tcagtgcata acttgaatag ccaattagca tcattgttaa
     1321 cgaaataatg atggtgctca ataggcgaat actaattttt tctttcaatt ttataaaata
     1381 aaaaatgagt atactgagta atacaagccc tagtaacaaa ctgcctaata gaaaagggat
     1441 acctaataaa atgagagatc cgatgaatga aactttaatg cgagtggcag atggtttttt
     1501 gctatataat tcaatgatag cccacactaa aacggcaatt gtagtaaaat aatagaaaaa
     1561 agctcctgta ttaaagctaa aacctaaggt attgacaaag aataattcgg caatgcctgc
     1621 gtttgctata aattgaggga taaaaccata catgatgaac ccaagtatag caaatgaaat
     1681 aagtagtgcc catgttccac ctttcaacga tactttttct gtttttctga aataatatac
     1741 caaaactaaa gctggtatag ttaataagtt aagtaagtga acaccaatag ataagcccat
     1801 taagtaagca ataagaatta ggtatttatc tgaacctgct ttatcgtgtt ggtcttccca
     1861 ttttaatata agcaaaaaaa caatagctgt aaaaaaggaa gaataagcat atacttcggc
     1921 ttcagtggct gagaaccaaa atgtgtccga gaaagcatag actagagatc cgattaatcc
     1981 gcttccgatt attgtaatag attgacttag tgttatttct gacccatttt gagcaatgat
     2041 tttttttgca atatgcgtaa ttgtccaaaa caaaaataaa atagtaaacg cacttaatgt
     2101 tgctgacatt aggtttacca tgatggctac ttgagaagga tcttttgcaa atagcgtgaa
     2161 aaaattacct gttaaattaa agaaaggagc cccaggaggg tgtcccactt gcagtttaaa
     2221 ggcggaggtt ataaattcac cacagtccca aaagctagct gtaggttcaa tagtaagggc
     2281 atacaccacc catgcaatta agaaacttat ccacccaaat agggtgttca tggttttaaa
     2341 tgatttcatt tttttcctga ttagttctga tttcccttaa aaaaagcctc acaaaagtac
     2401 taaattttac ccaaatacaa gtaggaaatg aatgattctg aaagttttta agaaatattg
     2461 ttgaaaacta aaaaactatt atgtatcttt gcggacattt tttaataatc tcttagaata
     2521 aattgtatgc aattaacaaa agaacaaaaa caagaaatct ttgagaaata cggaaagtcg
     2581 aatactgata ctggttcatc agaaggccag atagcattat tttcagcccg tatagctcat
     2641 ttgactgaac acatgaagtc aaatcacaaa gatcatagca cagaacgtgc tttagtaatg
     2701 ttggtaggaa aacgtcgtcg tttgcttgac tatctaaaag caaaagatat tgaacgttat
     2761 cgtgccatta tcaaagaatt aggtattaga aagtaactga tgtcctttta acaaaaaaag
     2821 tccgaatgaa gattcggact ttttttatgc gttgttctct gttttttgag gtattcaact
     2881 tgactgaaaa tgataataag accgttaata gttggtgatt ggactaagtg taatggattt
     2941 atcagtatga ttatacgttc ttaattaaat ttgttttact aaaataacat ttcttccttg
     3001 atatttttta tctttgtgtt ttgtatttca aatgtaagat tttgtggcaa aagttgtaga
     3061 aaccccttta atgaaacaat ataatcaggt gaaagcgaaa catcctgatg ctgtgttgtt
     3121 gtttcgtgtt ggcgattttt acgaaacatt ttctgatgac gctatacgtg cttctgaaat
     3181 tttaggtatt acgcttacac gtcgtgctaa cggagctgct agttatgtag aattagcagg
     3241 gtttccgcac catgccttag atacgtattt accaaaatta gtacgagctg gtttgcgcgt
     3301 tgctatttgt gagcagttag aagatccaaa aactgccaaa gggattgtca aaagaggcat
     3361 tactgaattg gtaacgccag gagtttcgta taacgagacg acgctcaatt ttaaagaaaa
     3421 tacatttgta gctgctgttc attttgagaa atcacgtgtt ggcgttgcct ttttagacat
     3481 ctctacggga gagtttttaa cggcagaagg atcctcggaa tatgtagata agcttctaac
     3541 gagtttttct ccaaaagaag ttttgtacga acgcggaaaa acaagtcttt ttgaacaatc
     3601 ttttggtacc cattttcttt catttacatt agaagactgg gtatttgtat cagacacagc
     3661 aaacgaacga ttacaaaaac aatttcaaac aaaaaatcta aaaggttttg gggtagttga
     3721 tttaccttta gggattattg cttcgggagc tattttacac tatttggagt ttaccaatca
     3781 tttacaaacg gctcacatta ctcatttgcg tagaatagag gaagaacgtt ttgtttggtt
     3841 agatagattt acaattcgta acctcgaatt gtatcaagcc atgcacgatg gtggaaaatc
     3901 attattggat gtgctagata aaaccttaac tcctatgggt gctcgtatgt tgaaaagatg
     3961 gttagctttt ccactaaaaa ataaagcagc catagagcat cgattgagta ttgtcagtta
     4021 tttttttaaa gacaaagata ttcaacaggt attgtatcaa caactttcgt taattggaga
     4081 tttagaacgt ttggtgtcta aaatagccgt tggacgcatc aatcctcgtg aattggtgca
     4141 gcttaaaatt gcgttaacag ctgttgaaac aattaaaaat tcaagtacac ttgttgataa
     4201 tgaggcatta gtatctatag ccgaacgttt gcagtcgtgt acacgaatgc gcgataaaat
     4261 tgaacaaaca attgcttcgg atgctcctat gttactacat aaaggtggtg taattaaaca
     4321 aggaattcac cctgaattgg atgaatatag acgtttagcc tattcgggta aagattattt
     4381 attacaaatt caacagagag aaagtgaagt aaccaatata cctagcctaa aaataagtta
     4441 taacaatgta tttggctact atattgaagt acgtaatacg cataaagata aagttccctc
     4501 aaactggatt cgtaagcaaa ccttagtcag tgcagaacgc tatataactg aagagttgaa
     4561 agaatacgaa gaaaagattg tgggggcaga agaaaaaatt gcccagatag aaggacaact
     4621 tttcaatgag ttagttgtat cgttgtgcga ttatattagt gccttgcaac tgaatgcttc
     4681 cttaattgca gaattagatt gcttgttgtc ttttgcgaat gtgtcagcta ataactccta
     4741 tattcctcca gttattactg aaacggacgt gttgtcaatc aaacaggggc gtcatcccgt
     4801 tatcgaaaaa caacttccta tgggggagtc ctatattgcc aatgatgtgt atttagatac
     4861 caatacccaa cagataatga tgattactgg acccaatatg gctggtaaat cagctttatt
     4921 aagacaaaca gctcttattg tattaatggc acaaataggt tgttttatcc ctgccgagag
     4981 tgccgaaatt ggtattgtag ataaaatttt tactagagtt ggtgctagcg ataatatctc
     5041 aatgggcgaa tctacgttta tggtcgaaat gagcgaagca tccagtattc taaataacgt
     5101 atcgcccaaa agtttaattt tattcgatga gctgggaagg ggaaccagta cctacgatgg
     5161 tatttctatt gcttgggcta tagtggaata cttgcacgag tatcctcaag caaaagccaa
     5221 aacattattt gctacccatt atcacgaatt aaatgagatg gaaaagactt tttcgcgaat
     5281 aaaaaacttc aacgttgctg taaaagaaat ggataacaag attattttta tgcgtaaatt
     5341 agtgccagga ggtacagaac atagttttgg tattcatgta gcaaaaatgg ctggtatgcc
     5401 aaagagtgtg gtaaaacgct ctaacgaaat tttagctcaa ttagaaaaag ataatcgcaa
     5461 aacaggacaa ccacaaaagc atattgatac agtggctcat caacgtgaag gcttgcagat
     5521 gagttttttc cagttagacg accccgtatt gatacaaatc agggatgaga ttaaaaacgt
     5581 tgatgtaaat aatttaacgc ccttgcaagc attgaataaa ttgaatgaga taaaaaaaat
     5641 aataacggga aaataac
//
LOCUS       DAOPUR010000025         5639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_14308_length_5639_cov_3.121956, whole genome shotgun sequence.
ACCESSION   DAOPUR010000025 DAOPUR010000000
VERSION     DAOPUR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14308_length_5639_cov_3.121956"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..3177)
                     /locus_tag="PKN90_00765"
     CDS             complement(124..3177)
                     /locus_tag="PKN90_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ84848.1"
                     /translation="MKYLHVSIFCLLFSLTVTPLFSQKSPFNTTAAELFADGKSFFYN
                     TNYAASARYFEEFLQFDQRDNDEMVREAEYYIARSAFELRKNDAQIKLEAYQTKYPYA
                     TDVELTNLYIGILEYESGRFKQAIRRFESIKTDRLRESDLVKLQFYKGYAYHQRENFE
                     KSRFEFQQVLRRGTNTPYYDAATYYFAYAEQRLGNFDSALTHFLRVENHPEFHTSAPF
                     FITQLYYQQGNCDKVLNYGKEIIKKYPKNNRRNEVLRMMGECSFLAKDYSSSIHYLTQ
                     YEQQVKRISREDFYMLGIAHYNMGEYEKAIANLSKTTNREDLLAQNSYLYLGMGYVKL
                     NDKKNARLAFETASRFTFDKGIQEEALYNFAVVSYEQSFSPFNESVVAFERFLTEFPS
                     SKRKDKVYEYLANSYLTTKNYGEAYRSIQNLKTDNKKIKEAEERILFNMGIVAVANNN
                     YQQATSHFEKILKGKSYNADFKTRSFYWYGESLLNTGYYTQAVKNFQTFLQSPKAQKL
                     AEYDIAHYSMGYTYYLLKNYTDAVTWFKRYIALKPDNNLMVLDANNRIGDSYYQNRQF
                     AEAKRYYNQAIATGAQLPGADYATFQKGFVAGLERNHNEKISTLRNLITTYPQSEWVD
                     DAYIEIGKTYVVLKENDKAIETFAEIEKKFSRNHQLIRQARLQIAMLQYNDGKTDAAL
                     ESYKQLVRTYPSSEEARISLEAIENILVEDNRVSEYTEFVNALGKGSIQIAPSREDSL
                     LFRAAQRVYSRNEIPQAISSFEKYLDTYPKGRYSTTARYLLASCYYTQKQPAKALPLY
                     KALLSDKENPNQEETLARAAEISYDLGQYAESMEYFKQLEAIGSSENKMVARIGLMRA
                     SYILKRYDETITYATNFISLHSNNLELLREAQHKRMLSYLELNKAQNALPDMQILAKD
                     TRNAFGAEAKYLLATHYYLAKQYESAEKEVFDFIDKGTPHHKWLAKAFILLADVYMEQ
                     DNYFEAKQYLLSLKSNYPKDDVEIASEINTRLEEIAKIENEKVNN"
     gene            3342..4298
                     /locus_tag="PKN90_00770"
     CDS             3342..4298
                     /locus_tag="PKN90_00770"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HNZ84849.1"
                     /translation="MRTYTNVKDLGNLQQALNEAFEVKNNPYKWDTLGKNKTLLMVFF
                     NSSLRTRLSTQKAGMNLGMNTMVLDINQGAWKLETERGVIMDGDTTEHILEAIPVMGS
                     YCDIIGVRSFARFENKEFDCQDTILKQFIQYSGKPVFSMEGAIGHPLQAFADLITIEQ
                     YKKTDRPKVVLTWAPHPKALPQAVPNSFADFMNEANVDFVITHPKGYELDEKFVRGAR
                     VEYDQRKAFEGADFIYAKNWSAFQDPHYGQILSKDRDWTVDTEKMALTNNAYFMHCLP
                     VRRNMIVTDDVIESPQSIVIPQAANREVSAQTVIKRMLEALK"
     gene            4307..4981
                     /locus_tag="PKN90_00775"
     CDS             4307..4981
                     /locus_tag="PKN90_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNZ84850.1"
                     /translation="MNLSVQISSSIASLLPHVVIGLLQCKVSNTSYNDELWTDIEDYS
                     KQFKQTYQLADINQRETIAATREAYRKTGKEPNRYRPSAEALCRRLVKGESLYRISTI
                     VDCINLISVKTGYSIGGFDRDSIIGNSLELGIGQAGEEFYAIGRGLLNIEGLPVYRDE
                     KGPIGTPTSDHERTKISLNTHHLVLIVNGFDGSITSVQQTLDFSRELLLKYTNASNIS
                     EKIVPA"
     gene            complement(4983..5453)
                     /locus_tag="PKN90_00780"
     CDS             complement(4983..5453)
                     /locus_tag="PKN90_00780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNZ84851.1"
                     /translation="MKKIQNPWTILDGYNCFACSPANEIGIKMEFFEEGEELICKWEP
                     SKHYEGFTNVLHGGIQATLLDEMGAWVVARKLQTVGFTSTLTIKYHKTVVINQPYIYI
                     KAKLTEVKRNLAFIEAHIYNYEEQLCASAEMCYFCTSQEAASSSFHFNGCSLEE"
     gene            complement(5472..>5639)
                     /locus_tag="PKN90_00785"
     CDS             complement(5472..>5639)
                     /locus_tag="PKN90_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNZ84852.1"
                     /translation="NTQHEAYQKAIDLLLELRLEAKRNKDFALSDKIRNELTTLGFSI
                     KDTKEGFEWSL"
BASE COUNT         1694 a         1004 c         1014 g         1927 t
ORIGIN      
        1 attgcgaaaa actcaacgat gtattggcta aaaatagtac caaaggtagt atatagatta
       61 ttcttttcat acaattttgt ttataggatt gtgattacac acgattctgg cttactaact
      121 ttattaatta ttcacttttt cattctcaat tttagcaatc tcttccaaac gagtattgat
      181 ttctgatgct atttctacat cgtctttggg ataattactt tttaaactta ataagtattg
      241 ttttgcttca aaataattat cctgttccat atatacgtcg gcgagcaata taaaggcttt
      301 ggctaaccat ttgtgatgcg gagttccctt atctataaaa tcaaacactt ctttttccgc
      361 tgattcatat tgtttggcta ggtagtaatg tgtagcaagt aagtatttag cttctgctcc
      421 aaaggcattt cgtgtatctt tagctaagat ttgcatatct ggcagtgcat tttgtgcttt
      481 atttagctct aaataactca acatacgttt gtgttgtgct tcgcgaagca attcaaggtt
      541 gttgctatgc aaggatataa aattagtggc atacgtaatt gtttcatcat agcgtttcaa
      601 aatatacgat gctctcatca gtccgatgcg tgctaccatt ttattttcgc tcgaaccaat
      661 cgcttcgagt tgtttaaaat attccattga ttctgcatat tgccccaaat catagcttat
      721 ttctgccgcc cttgctaagg tttcttcctg atttggattc tctttatcag atagcagtgc
      781 tttatataaa gggagggctt ttgcaggctg tttttgtgta taataacaac ttgctaacaa
      841 atagcgagca gtagtactat aacgcccttt gggataggta tctaaatatt tctcaaaact
      901 tgaaattgct tgtggtattt cgttgcgcga atagacacgc tgagccgctc taaacaataa
      961 tgagtcttct ctcgaagggg ctatttggat agagcctttt cccaaagcat taacaaactc
     1021 ggtatattca cttacacgat tatcttctac caaaatattc tcaatagctt ccaacgatat
     1081 tctagcctct tcgcttgagg gataggtacg tacaagttgt ttgtacgact ctaaggctgc
     1141 atctgttttt ccatcattgt attgcaacat cgcaatttgt aagcgtgctt gtcgtataag
     1201 ctgatgatta cgggagaatt tcttttcaat ttctgcaaag gtttcaatag ccttatcgtt
     1261 ttcttttaat accacgtagg ttttaccaat ttcaatgtaa gcgtcatcta cccattctga
     1321 ttgaggatac gtagtaatta aatttcgtaa ggttgatatt ttctcattat gatttcgttc
     1381 taatccagca acaaatcctt tttggaatgt agcataatca gctccaggta attgagcccc
     1441 tgttgctatg gcttgattat aataacgttt tgcctctgca aattgtctgt tttggtaata
     1501 agaatcgcca atacgattat tggcatccaa caccatgagg ttgttgtctg gttttaatgc
     1561 aatgtatcgt ttaaaccatg ttacagcatc tgtgtagttc ttcagtaaat agtaggtgta
     1621 ccccatacta taatgagcaa tatcgtattc tgctagtttt tgtgctttag gtgattgtaa
     1681 aaaggtttga aaattcttta cagcttgggt ataatatcca gtattcaaca aactttctcc
     1741 ataccagtaa aatgaacgag ttttaaagtc cgcattgtat gatttaccct tcaatatttt
     1801 ctcaaaatgc gatgttgctt gttggtaatt gttattggca acagccacta tacccatatt
     1861 gaataaaata cgctcttcag cttctttaat ttttttattg tcagttttca ggttctgaat
     1921 agaacgatat gcctctccat aattttttgt agttaaatag ctgtttgcta ggtattcata
     1981 cactttgtct ttgcgtttgg atgaggggaa ttcggttaaa aaacgctcaa aagcaactac
     2041 tgattcatta aatggagaaa atgactgttc gtaactaact accgcaaaat tatacaaggc
     2101 ttcttcttgt atgcctttat caaaggtaaa acgagaagct gtttcaaaag ctagtcgtgc
     2161 atttttctta tcgtttagct tgacatatcc cattcccaag tataggtaac tattttgtgc
     2221 taataaatct tctctgtttg ttgttttaga caagtttgca atagcttttt catattcacc
     2281 catgttgtaa tgagctatcc ctaacatata aaaatcttca cgcgaaattc gttttacttg
     2341 ttgctcatac tgagtaaggt aatgtatgct tgagctataa tctttggcaa gaaaactaca
     2401 ttcgcccatc atgcgtaata cttcattacg tcgattgttt ttagggtatt tctttataat
     2461 ttcttttccg taatttaata ctttatcaca atttccttgc tgataatata gttgggtaat
     2521 aaaaaatgga gcactagtat gaaattcagg atgattttcg acacgtaaaa aatgagtaag
     2581 agcactatca aaattgccta atcgttgctc tgcataagca aaataatagg tagctgcatc
     2641 gtaatagggc gtattcgtgc ctctgcggag tacttgctga aattcaaaac gagatttttc
     2701 aaaattttct cgttgatggt atgcatagcc tttgtagaat tgcaatttaa ctagatccga
     2761 ttcgcgtaat ctatctgttt ttatgctctc aaaacgacga atggcttgct taaatctacc
     2821 agattcgtac tctaaaatgc caatatatag attcgtaagt tctacgtctg ttgcataagg
     2881 gtattttgtc tgatatgcct caagtttaat ttgtgcgtca ttttttctaa gttcgaatgc
     2941 tgaacgtgca atgtaatact ctgcttcacg caccatttcg tcattgtctc gttggtcaaa
     3001 ttgtaaaaac tcttcaaaat atcgtgccga tgcagcataa ttagtattgt agaaaaagga
     3061 cttaccgtct gcaaaaagtt cagcagcagt agtgttaaag ggtgattttt gactaaaaag
     3121 aggagtaacg gttaaactaa atagtagaca aaaaatggaa acgtgtaagt atttcataag
     3181 tgttcgattt tttctgaatc aatgtataac gctagaacga ataaaaaatc agattattac
     3241 ttgcaaaggt ataaattata tttgaattta aaaacataaa aatcctcaat caatttacgt
     3301 tttttgtgta cctttgtgtt ttaatcaatt cgtcattcat tatgagaact tacaccaacg
     3361 taaaagattt aggaaatttg caacaagcat taaatgaggc ttttgaagta aaaaataatc
     3421 cttataaatg ggacacacta ggaaaaaaca aaactttgtt gatggtgttt tttaattcta
     3481 gtctacgtac ccgtttaagt acacaaaaag caggcatgaa tttaggtatg aacaccatgg
     3541 tactcgacat taatcaagga gcttggaaat tagaaacaga acgcggtgta attatggatg
     3601 gcgatactac agagcacatc ttagaagcta ttcccgttat gggttcttac tgcgatatta
     3661 tcggtgttcg ttcgtttgct cgttttgaaa acaaagagtt tgattgtcaa gataccattc
     3721 taaaacaatt tatccaatat tctggcaaac ccgtatttag catggaggga gctatcgggc
     3781 atccattgca ggcttttgcc gatttaatta ccattgaaca atacaaaaaa acagatagac
     3841 caaaagttgt gctcacctgg gctcctcacc ctaaggcttt gcctcaagct gtacccaatt
     3901 cgtttgccga ttttatgaac gaagccaatg tcgattttgt tattacacac cccaaagggt
     3961 atgagcttga tgaaaagttt gtaagaggtg ctcgtgtaga atacgatcaa cgcaaagcgt
     4021 ttgaaggggc tgattttatc tatgctaaaa actggtcagc ttttcaagac ccacattatg
     4081 ggcaaatctt gtctaaagat agagattgga cagtagatac cgaaaagatg gcacttacca
     4141 ataatgctta ttttatgcac tgtctgcccg taagacgcaa tatgattgtt acggacgatg
     4201 taattgaaag cccgcaatca attgtaatcc ctcaagccgc taatagggag gtgtctgcac
     4261 aaactgttat taagcgtatg ttagaggcat tgaaataagc tttagtatga acctttcagt
     4321 acaaattagt agtagcatcg catctttatt gccacatgtt gttattggtc tgttgcaatg
     4381 taaggtgtct aatacgtctt ataatgatga attatggaca gatattgagg actattctaa
     4441 gcaatttaaa caaacatacc aactagctga cataaaccaa cgagaaacta ttgctgctac
     4501 acgtgaagcg tatagaaaaa caggaaaaga accgaataga tatagaccct cagccgaagc
     4561 tctttgtcgc agattagtga aaggagaatc attgtatcgc atttcaacca tagtggattg
     4621 tattaattta atctcggtta aaacgggcta ttccatcggt ggattcgata gagatagcat
     4681 tattgggaat tctcttgaac ttggcattgg acaagctggt gaagaatttt atgcaatagg
     4741 aagagggttg ttaaatatcg aaggattacc cgtgtatcga gatgaaaaag gaccaatcgg
     4801 aactcctact agcgatcacg aacgtacaaa aatttctctc aatacacacc atcttgtgct
     4861 aatagtaaat ggttttgatg gctcaattac ttctgtgcaa caaacactcg atttttccag
     4921 agaattgctt cttaagtaca caaatgctag caatatatca gaaaaaatag taccggcata
     4981 gcttactcct ctaacgaaca tccgttaaag tgaaaagaag aagatgctgc ttcttgtgag
     5041 gtgcagaaat aacacatttc tgctgaggca cacaattgct cctcgtaatt ataaatatga
     5101 gcttcaataa atgctaaatt gcgttttacc tctgttagtt tggctttgat ataaatataa
     5161 ggctggttaa ttacgactgt tttatggtat ttgatagtta atgtactcgt aaaaccaacc
     5221 gtttgtaatt tgcgagccac cacccaagct cccatttcat ctaacaaggt ggcttgtata
     5281 cctccatgca atacgttggt aaatccttca tagtgttttg aaggctccca tttgcaaatc
     5341 aactcttctc cttcttcaaa aaattccatt tttattccta tctcatttgc tggactacaa
     5401 gcaaagcaat tgtagccatc tagaattgtc catggatttt gtatcttttt catgttacaa
     5461 ggtttttatt cttatagcga ccattcaaat ccttcttttg tatctttgat gctaaaacca
     5521 agagttgtta attcgtttct gattttatct gatagggcaa aatctttgtt tcttttagct
     5581 tctaatcgta gctcaagcaa caagtcaatt gctttttggt atgcttcatg ttgagtatt
//
LOCUS       DAOPUR010000026         5589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_14478_length_5589_cov_2.560354, whole genome shotgun sequence.
ACCESSION   DAOPUR010000026 DAOPUR010000000
VERSION     DAOPUR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14478_length_5589_cov_2.560354"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..2124)
                     /locus_tag="PKN90_00790"
     CDS             complement(52..2124)
                     /locus_tag="PKN90_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNZ84853.1"
                     /translation="MYKKERLLFDKFAITQHDKFIRINGIASKLEDDWLYVDINQFNL
                     EYLSELLQLKGIHLSGIATGSITAQQAFNKPILNASINATDFGLNSAPFGNVEVTANY
                     NYEKEQIDIEGLITNHKLQQSHVSGYISPVRNDMLLRADMHEVNVKFLDPYLSSFAKD
                     IEGEATGVIYIGGDLRAIEVWGKAFINKAKLHIDYLKTDVYFSDYITLTKNSILLSDI
                     TIADKKGNKGTVNGKITHKAFSEFLFNIDLLLKNMLVYNTTENDNPDFYGTVYASGIA
                     NIYGNVDLLSIDVAGKTEKNTVFYIPINTSTSALEGDFITFVNRKQLSTTTRERRRTQ
                     QSESTESKLRISVQIEATPDAEAILILDDRTREYIQGNGSGNIRLSLEPNENLKMYGN
                     YTLNEGKYFFAMQSAIRKTFEIKDGSSIAWDGNPYNGAIDIMATYQVSASLIDLLDAS
                     MLQDVKRLTVPVLCKAHLTGELQQPTIKLDLELPTADDELKRRVQNIVNSDEMMSNQM
                     VFLLMLGHFYNPQLLTNTQPNTAGMATMIASATLSSQLNYWLSQISNDVNLGVNYRQS
                     EEGDITSREIEVALTTNLLDNRLIINSNVGYKEDAFTETNFIGDFDIEYKINRSGRLR
                     LKAYSHSNDKYYVRNALTTQGVGVIYREDFASGKTLWNYYRRLFKLKAKNKTSQEGIV
                     EEPNENIK"
     gene            complement(2146..4467)
                     /locus_tag="PKN90_00795"
     CDS             complement(2146..4467)
                     /locus_tag="PKN90_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84854.1"
                     /translation="MLATLLLNTKPVQKYLLHQATHYLSNKIGSEVSISTVTYNLFTT
                     FEFSDIQIKDKERNDLLTIEHIDIKFKILPLFKQKFIVKSVSFIQPSINILRDSAGIS
                     NFQYILDAFAKNETDSIESKFVFDIRNITIKKGELKLTNIGTARKTQTFDPNHIHIWD
                     LNSEMRLHKIKSDSIVFELKSCSFEEQSGLIVKNLYADASFGKKYIHINDLSIKLPSS
                     IMEFEKLSIDYAHQENINSIVHMLDKSSIVLNSKKVVITGRDIACFVPRLAHLKNPIN
                     IEVNAQGANNTIELSKCIIKYGDQISLDASMQLAGINNMDETFIYADIHSISAHSTAI
                     ETVVSDIMQKPFILPKEIRNLGQMHYTGNIAGFFNNLVVYGKLRTDIGILSTDILLGI
                     DIQKNDIAYSGNLRSSRFQLTKMLGKDSGFGNIQFRVDVNGTKTHENKHNGIVKGNVQ
                     SIDFNKYTYKNIKLDGEYNNNGFNGKIELNDDNGQIKFDGDIDFSRKIPLMNFVAQVN
                     NVRPYKLQLTEHNPDLSLSFTIASSIQGTTIDNVIGNVFIQDINLHNASDTFEIKQVE
                     MKSFIEDEKQCLRITSDFLNATILGKITLSSIANNFLHIVHTEVPNISTLQRDKKLSG
                     NNFTFEANIEPIDQLSKVLLWNISTKEPTFIQGSFNDFTNIFSINAQSKGIQLNKNYL
                     YFATIDLHNSTSGIDLDANVGLKMPKDSLLVNVTSKILNNQGNTSISWKNSYGKEFKG
                     KLESVLDIKKNEDNEVETNIHILPTEMVVNDSI"
     gene            4649..>5589
                     /gene="tsaD"
                     /locus_tag="PKN90_00800"
     CDS             4649..>5589
                     /gene="tsaD"
                     /locus_tag="PKN90_00800"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNZ84855.1"
                     /translation="MDNITILGIESSCDDTSAAVIRNGELLSNVIANQDVHIAYGGVV
                     PELASRAHQLNIVPTIHQAIKNASVSLSEIHAIAYTRGPGLMGSLLVGTSFAKGLALS
                     LGVPLIDVNHLKAHVLVHFIHDNTPNFKPPTFPFLCLLVSGGNSQIVLVKSYSEMEII
                     GQTIDDAAGEAFDKCAKILGGPYPGGPFVDVMAQQGDAYAFNLNKPRIGGYQYSFSGL
                     KTSFLYLVRDEMKKNPAFIEQRKTDLCASFQRTVVEILMEKLLKASQDYHISTIALAG
                     GVSANSALRKAVENYAHIYNWTTFVPKLAFTTDNAAMV"
BASE COUNT         1679 a          907 c          910 g         2093 t
ORIGIN      
        1 tacgagatga ttgcagatgt gaattaccac caattgtttg tgttatcatg tttattttat
       61 attttcatta ggttcttcaa ctattccttc ttggctagtc ttgtttttcg ctttgagttt
      121 aaataatctt cgatagtaat tccaaagagt tttccctgaa gcgaagtctt cgcggtatat
      181 gacaccaaca ccttgtgtag taagagcatt acgcacataa tatttatcgt tagagtgaga
      241 gtaagctttg aggcgtaacc tgccagaacg attgatttta tattcgatgt caaaatcgcc
      301 aataaaatta gtttctgtaa aggcgtcttc tttgtatccc acgttgctat ttattataag
      361 cctattatca agtaaattag tcgttaaagc tacttctatt tctctactag taatgtctcc
      421 ttcttcactt tgacgataat ttacaccaag gtttacgtcg ttactaattt gagataacca
      481 ataattcaat tggcttgaaa gtgtggcaga ggctatcata gttgccatcc ctgcagtatt
      541 tggttgtgtg ttagtaagta gctgaggatt atagaaatgc cctaacatca ataaaaagac
      601 catttgattg ctcatcattt cgtcagagtt tactatattc tgcactcttc gttttaattc
      661 atcgtcagca gtaggtagtt ctaaatctaa tttaatagta ggttgctgta attctcctgt
      721 taaatgagct ttacataaaa caggaacagt gagtcttttt acatcttgca acatactggc
      781 atctaataaa tcaataagag atgcagatac ttgataggtc gccattatgt cgatggctcc
      841 attatacgga tttccgtccc aagctatact acttccgtct ttgatttcaa atgtttttct
      901 gatagcactt tgcatagcaa aaaaatattt tccttcattt agggtgtaat taccatacat
      961 ttttaagttt tcgtttggct ctaagcttag tcgaatgttg ccactgccat taccttgaat
     1021 gtattctcgt gttctatcat ctaaaattag aattgcttca gcatctggtg tggcttcaat
     1081 ttgtactgat atgcgtagtt ttgattcagt tgattctgat tgttgggtac gtcttctctc
     1141 tcttgttgtt gtagaaagtt gttttctgtt gacaaaggtt ataaagtctc cttctagtgc
     1201 actagtactt gtgttgattg gaatatagaa gaccgtattt ttttctgttt tgcccgctac
     1261 atcaattgat agtaaatcca cattcccata tatatttgct atacctgatg cgtatactgt
     1321 tccataaaaa tcaggattat cattctctgt tgtgttgtat accaacatat ttttgagcaa
     1381 taaatcaatg ttaaataaaa actcagaaaa tgctttatgt gtaatttttc cgtttacggt
     1441 acctttgttc ccttttttat ccgcaatggt tatgtcagat aacaaaatag aattttttgt
     1501 taaggttata tagtcagaaa aatatacatc tgttttaaga taatcaatat gtaatttagc
     1561 tttatttatg aaagcttttc cccatacttc tattgctcgt aggtcgccac caatgtatat
     1621 tacacccgta gcttctcctt caatatcttt ggcaaatgaa gataagtagg gatctaaaaa
     1681 tttcacattc acttcatgca tatcagcacg taaaagcata tcattgcgta caggagatat
     1741 atatccactt acatgagatt gttgaagttt gtggtttgtt atcaatcctt caatatctat
     1801 ttgctctttt tcgtaattat agtttgcggt tacttctaca ttgccaaaag gggcactatt
     1861 taaaccaaaa tctgtagcat tgatagatgc atttagtatt ggtttattga aagcttgctg
     1921 tgcagtaatg cttccagttg caataccaga cagatgtatg cctttaagtt ggagtagctc
     1981 acttaaatat tctagattaa attggttgat atccacatac aaccaatcat cttctaattt
     2041 tgatgctatt ccatttattc gaataaactt atcgtgctgt gtaatagcaa atttatcaaa
     2101 caataaacgt tcttttttat acactaatgt actgtttgta atatgctata ttgaatcatt
     2161 taccaccatt tctgtaggta aaatgtgaat attcgtttct acctcgttat cttcattttt
     2221 tttgatatca agtacacttt ctagtttacc cttaaattct ttgccatagc tatttttcca
     2281 agaaatagat gtatttcctt gattattaag aattttgctt gttacattga ctaataaact
     2341 atcttttggc atttttaatc ctacattggc atcaaggtcg atacccgaag tactattatg
     2401 taaatcaatc gtagcaaaat aaagatagtt cttgtttaat tgtattcctt tgctttgtgc
     2461 gttgatagaa aaaatatttg taaaatcatt aaaagaaccc tgtataaaag ttggttcttt
     2521 tgtgctaata ttccataata atacttttga taattgatct attggctcaa tgttagcctc
     2581 aaatgtaaaa ttatttccac ttaatttttt atcccgttgt aaggtgctta tatttggcac
     2641 ttcggtgtgt actatatgca gaaaattatt cgcaatagac gatagtgtaa ttttaccaag
     2701 aattgttgca ttcaaaaagt cagatgttat tcgcaaacat tgtttttcat cctcgatgaa
     2761 cgacttcatt tctacttgtt ttatttcaaa tgtatcgctt gcattatgga ggttaatgtc
     2821 ctgtataaat acattaccaa taacattatc aatagttgta ccttgtatag aggaagcaat
     2881 tgtaaacgaa agactcaagt caggattatg ttcagtaagt tgcaatttgt acggacgcac
     2941 attattcact tgggcaacaa aattcatcaa aggaattttt cgagaaaaat caatgtcacc
     3001 atcaaatttt atttgtccgt tatcatcgtt taattcaatt tttccattaa acccattgtt
     3061 attgtattct ccatctaatt tgatattttt atacgtatat ttgttgaaat ctattgattg
     3121 tacattccct tttacaatac cattgtgttt gttttcatgt gttttggtgc catttacatc
     3181 taccctaaat tgaatatttc caaaaccaga atctttacct aacatttttg ttagttgaaa
     3241 acgagaagaa cgcaaatttc cactatatgc aatgtcattt ttttgtatat caatacctaa
     3301 taatatatcc gtggataata tgcctatatc tgtacgcagt tttccatata ccaccaaatt
     3361 gttaaaaaat ccagcaatat ttccagtata atgcatctgt cccaaattcc gtatttcttt
     3421 tggtagtata aatggttttt gcataatatc tgaaaccact gtttctattg ccgtactatg
     3481 tgcagagatt gaatgaatgt cggcatatat aaatgtttca tccatgttat taataccagc
     3541 caattgcatt gatgcgtcca aagatatttg atctccatat ttaataatac atttgctcag
     3601 ttctatggtg ttgttagctc cttgtgcatt tacttctatg tttatcggat tttttaaatg
     3661 tgctaagcga ggaacaaaac aggcaatatc tctacctgtt ataactactt tctttgagtt
     3721 gagcacaata gaagatttgt ctagcatgtg gacaatagaa ttgatatttt cttgatgagc
     3781 ataatcaata gaaagttttt caaattccat aatggaagaa ggtagcttaa tagataaatc
     3841 attgatatgg atatattttt taccaaacga agcatcagca tataagtttt ttacaattaa
     3901 tccagattgt tcctcaaaac tacatgattt caattcaaaa acgatagaat ccgatttaat
     3961 tttgtgtaaa cgcatttctg agttcaaatc ccaaatgtga atatgattag gatcaaaagt
     4021 ttgggttttg cgtgcggtac ctatattcgt tagttttagt tctccctttt ttattgttat
     4081 atttctgatg tcgaatacaa acttactctc aatactgtct gtttcatttt tagcaaaagc
     4141 atccagtatg tattgaaagt tactaatccc cgcactatct cttagtatat tgatagatgg
     4201 ttgaatgaaa gagactgatt tgacaataaa tttttgttta aataagggaa gtattttaaa
     4261 cttaatgtct atatgttcaa tagttagtag gtcattacgt tctttatctt ttatctgaat
     4321 atctgaaaat tcaaatgtcg taaataaatt atatgttaca gttgaaatag aaacttcgct
     4381 tccaattttg ttggaaaggt aatgggtggc ttgatgcaac aaatattttt gtactggttt
     4441 ggtgttgagt aataaagttg ctagcaaaag tagtcctgct acaattgcta acgtaatact
     4501 tgttatagtt acatattttt taataacttt gcactttaat ttcaacctac aaaaataaca
     4561 ataaaacctg ttaaataaaa tagaaaacca ataagcagta gatattttgt ttttttaagg
     4621 ataatttgag tttctttttc tgtaatttat ggataatatt actatacttg gaattgaatc
     4681 atcgtgcgat gatacttctg ctgctgttat tcgtaacggg gaattgttgt ctaatgttat
     4741 tgctaatcaa gatgtacata ttgcttatgg tggggtagtt ccagaattag cttcaagagc
     4801 acatcaactc aatattgtac caaccataca tcaagctatt aaaaatgcct cggtttcgtt
     4861 aagtgagata catgccatag cctatactcg tggaccaggg ctcatgggtt ctttactcgt
     4921 aggtacttct tttgcaaaag gattagcatt gtcattagga gttcctttaa ttgatgtaaa
     4981 tcatttaaaa gcacatgtac tggttcattt tattcatgat aatactccaa attttaagcc
     5041 accaactttc ccttttttat gtttgttggt gtcagggggt aattcgcaaa ttgtactggt
     5101 gaaatcatat agcgagatgg aaattatagg acaaaccatt gatgatgctg ctggagaggc
     5161 ttttgataag tgtgccaaaa tacttggcgg accttatccc ggtggtccgt ttgttgatgt
     5221 gatggctcag caaggagatg cttatgcttt taaccttaat aaacctcgaa taggcggtta
     5281 tcaatatagt tttagtggac ttaaaacatc ttttctctat ttagttaggg atgaaatgaa
     5341 aaaaaatcct gcgtttattg aacaacggaa aaccgattta tgtgcttctt ttcaacgtac
     5401 agttgtggaa attttaatgg agaagttgct taaagcatct caagactatc atatatcaac
     5461 gatagctctt gctggaggtg tatctgctaa tagtgcgtta agaaaagcag tcgaaaatta
     5521 tgctcatata tataattgga ctacctttgt ccccaaatta gcttttacaa cagataatgc
     5581 tgcaatggt
//
LOCUS       DAOPUR010000027         5550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_14610_length_5550_cov_3.811647, whole genome shotgun sequence.
ACCESSION   DAOPUR010000027 DAOPUR010000000
VERSION     DAOPUR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14610_length_5550_cov_3.811647"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1461
                     /locus_tag="PKN90_00805"
     CDS             154..1461
                     /locus_tag="PKN90_00805"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HNZ84856.1"
                     /translation="MTKHLISFFFSILLIGLASCLNEKEDLSNNPHPSAVSIQTNTLA
                     PDIESTVFTIDETNLTISNSDSIAYDSRIDKLVPVFSFAEGVEQILINGTIWNTTDSI
                     DFSSPVIIQFKAKDKLTTAEYTFKVNKHTVDPEAITWTKRADNFTNLTNIEEMAGLWF
                     QSQAILFVQKGNETFVLKSNNGQTWTEATSSNIPLEVSTILVHKNAILAISKNHHLIT
                     SVDGINWSIISSSDEISFINILCSLQNKAWIVGDKNNITYIYSYNSESETITEVTTVS
                     DKLPYIEVSKAMMKTKNGIEKSVLISGKTNNDTPVYSVFSSMTGDYWVNVITETASLT
                     FGERIHATAIWLDSKLLLFGGSTANNNNLLDVYISSDEGYTWEIASSNKQLPVEIGAR
                     KKMIGFVDSNNTLWLIGGIDALGNIQSDVWQGRLNKFDFIKQD"
     gene            1463..1909
                     /gene="coaD"
                     /locus_tag="PKN90_00810"
     CDS             1463..1909
                     /gene="coaD"
                     /locus_tag="PKN90_00810"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNZ84857.1"
                     /translation="MSKTALFPGTFNPFTIGHYSLVKRALTIFDKVIIAIGINSTKEN
                     NLDEIEENKKQIADLFKNENVKVITYTNLTADIVKAEKASCIVRGIRGISDYEYEKNM
                     ADINKELFEIETVFLFSDLKYAHISSSLIRELKRFGKEYKHLLPES"
     gene            complement(1895..2953)
                     /locus_tag="PKN90_00815"
     CDS             complement(1895..2953)
                     /locus_tag="PKN90_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNZ84858.1"
                     /translation="MKAIIINPIELLTSLLVIPSISKSEQEKSDFLVNFLKQHDCDVQ
                     RHKNNLFCFAKSYDENKPTLMLNSHMDTVRPSDSWTFPPYQPTLLDGRIIGLGANDAH
                     ASLVSLITVFLMLKDTEQSYNLLLAISAEEEISGVNGMESLLTVLPKIDFAIVGEPTQ
                     MNLAVAEKGLMVLDCVAKGQSGHAAREEGVNAIYKAIRDIEWFKNYQFEKESDLLGAV
                     KMSVTMIQAGKQHNVVPDACAFTVDIRVNECYSNQQVFDIVKQYVEADVTPRSFRLNS
                     SAIDLNHVFVKRFLSIKPTIFGSATLSDQTFMQFPSVKIGVGDSARSHTADEYIFISE
                     IEEGIGLYKQLIDGLTLR"
     gene            complement(2958..3734)
                     /gene="argB"
                     /locus_tag="PKN90_00820"
     CDS             complement(2958..3734)
                     /gene="argB"
                     /locus_tag="PKN90_00820"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNZ84859.1"
                     /translation="MKQSLIIIKVGGKIVEESASLASFLKDFAHTEGYKVLVHGGGRS
                     ATKLSEKLGIESKMVDGRRITDKQTLDVVTMVYGGLVNKNIVAGLQALGVNALGLTGA
                     DMNIIESVKRPIKEVDYGYVGDVKHVNAQIVASLIQQGIVPIVAPLTHDGAGSMLNTN
                     ADTIASELAKSLSTYFDVTLVYCFEKNGVLSDEMDDNSVIPLITPSLFNKYVHQGIIK
                     GGMIPKLENAFQAIQAGVSKVVITNATAIGKDAGTVVLTD"
     gene            3882..4430
                     /locus_tag="PKN90_00825"
     CDS             3882..4430
                     /locus_tag="PKN90_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ84860.1"
                     /translation="MQKRKITELNRISVEEFQSKTKLPLVVILDNIRSLHNIGAIFRT
                     CDAFLIEEIYLCGITATPPNAEIHKTALGAENTVKWVYFESIMDAIRQLQTKGYSVFA
                     VEQVKQSIPLNELKIDVTKKYAIIVGNEVKGVQQVAVDMCNGAIEIPQFGTKHSLNVS
                     IAAGIVIWDLVKELSTSQSERG"
     gene            4427..5011
                     /locus_tag="PKN90_00830"
     CDS             4427..5011
                     /locus_tag="PKN90_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNZ84861.1"
                     /translation="MSLSVCIYCASSNAINSVYYQETEKLARLLAQNNVNVKFGGGSS
                     GLMGKIADVYVEENNDNIVGIIPKFMCEEGWQHQQLKQLIVTETMHERKQKMIENVDA
                     AIALPGGIGTLEEVLEVLTWKQLGIFLKPILLVNINGYFDPLLIALEHAVSENFMRKE
                     HLQLFTIISSVENILQDIQKNPTYDVSIRKIAQI"
     gene            5018..>5550
                     /locus_tag="PKN90_00835"
     CDS             5018..>5550
                     /locus_tag="PKN90_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNZ84862.1"
                     /translation="MPEDSLVQITNNYPPLLQSPDSITNHVPVWDDILFSSTMHIDST
                     RILIPEGMESIWRTTSFFHSDSVIALLLSIFILLCITIAFFRKAKTHYFTSIFKHKNE
                     TEQINLTVLEQWKQVLYSLFGLIGYTSIALYLVVEKSTIINYYETWEIGGWLFVALFL
                     YFFIKYILLKLYVYKFFS"
BASE COUNT         1925 a          962 c          911 g         1752 t
ORIGIN      
        1 aagtggaaaa aagacaaaaa aaaacaaaat caagcaaaaa tttatacctt tgcaactaga
       61 attctcatat atcatagaat gagacttcat gttatatgtt agttaatgca aaattcatct
      121 atttctctta ttacataaca atgttaattt accatgacaa aacatcttat ttcatttttt
      181 ttctctatcc tactaattgg tcttgcatct tgtttaaatg agaaagaaga tttatcaaac
      241 aatccacatc cgagtgcagt aagtatacaa acaaatacgc ttgccccaga tattgaatcg
      301 acagtgttta cgattgatga aaccaatctt accataagca atagtgattc aattgcatat
      361 gatagtagga ttgataaatt ggtacctgta ttttcttttg cagaaggggt agaacaaatt
      421 ctgataaatg gaactatttg gaatacaaca gattcgatag atttttcttc tcctgttatt
      481 attcaattta aagcaaaaga taaactaacc acggcagaat atacttttaa ggtaaacaag
      541 cacacagtag atccagaggc tattacatgg actaaaagag cagacaattt taccaactta
      601 acaaacatag aagaaatggc aggcttatgg ttccaatcac aagccatact ttttgttcaa
      661 aaaggaaatg aaacatttgt gttgaaaagc aacaacggac aaacatggac agaagcaaca
      721 tcaagtaata ttccgttgga ggtcagcacc atacttgttc ataaaaatgc catacttgcc
      781 atttcaaaaa atcatcatct tataacatcg gtagatggga ttaactggag tataataagt
      841 agtagtgatg agatttcatt tattaacatc ttatgttcat tacaaaacaa agcttggata
      901 gttggagata aaaacaatat aacatatata tactcatata attcggaatc tgaaaccata
      961 actgaagtta caactgtttc tgacaaatta ccgtatattg aggtttcaaa ggcaatgatg
     1021 aaaacgaaaa atggtattga aaaatcagta ttgattagtg ggaagaccaa caacgacact
     1081 cctgtatatt cagtattttc atctatgact ggagactatt gggtaaatgt aataacagaa
     1141 acggcatcat taacttttgg agaacgtata cacgctactg ctatctggct agacagcaag
     1201 ctattgttat ttggcggatc aaccgccaac aacaacaatt tgttagatgt atacatttca
     1261 tcagacgaag gctacacttg ggaaatagcc tcatcaaaca aacaactccc tgtggagatt
     1321 ggtgcaagaa aaaagatgat tggttttgtg gatagcaaca acaccctttg gctaattgga
     1381 ggtatagatg ctttgggcaa catacaatct gatgtatggc aaggtagact aaacaaattt
     1441 gattttataa aacaagattg atatgtcaaa aacagcacta tttcctggta cattcaaccc
     1501 ttttacaatt ggacattata gtttggttaa acgtgctcta accatatttg ataaggtaat
     1561 aattgctatt gggattaact caactaaaga gaataatctt gatgaaatag aagaaaataa
     1621 aaaacaaata gccgatctat ttaaaaatga gaatgtaaaa gtaattacct atactaattt
     1681 aaccgcagat atcgtaaaag cagaaaaagc atcttgtatt gttagaggaa ttagaggaat
     1741 aagcgattat gagtacgaaa aaaatatggc tgatattaat aaagagttat ttgaaatcga
     1801 aaccgtattt ttattttctg atttgaaata tgctcacatc tcctcgtctc ttatcagaga
     1861 acttaaacgc tttggcaagg aatataaaca cctattacct gagagttaaa ccatcaatta
     1921 attgtttata taaacctatt ccttcttcta tttcagaaat aaaaatatat tcatctgccg
     1981 tatgagagcg ggctgaatcc cctacaccaa tttttacact tggaaattgc ataaatgttt
     2041 gatcagacaa ggtagctgaa ccaaatattg ttggttttat cgaaagaaat ctctttacaa
     2101 acacatggtt taaatctata gcagaagaat taagacgaaa agatcgtggt gtaacatctg
     2161 cttctacata ttgtttcaca atgtcgaaca cctgctgatt ggaataacac tcgttgacac
     2221 ggatatccac tgtaaacgca caagcatcag gcactacatt atgctgtttg cccgcctgaa
     2281 tcattgttac agacattttt actgctccca ataaatcaga ttctttttcg aattggtaat
     2341 ttttaaacca ttcaatatcg cgaattgcct tataaatggc atttacgcct tcttcgcgtg
     2401 cagcatgacc actttgtcct ttggcgacac aatcaagcac catcagccct ttttctgcta
     2461 ccgccaaatt catttgggta ggttctccta ctattgcaaa atcaattttt ggtaatacgg
     2521 ttaataagct ttccatacca tttactccag aaatctcctc ttcagccgaa atggctaata
     2581 ataaattata ggattgttct gtgtctttta acattaaaaa aacagtgatt aatgatacta
     2641 aagatgcatg ggcatcatta gctcctaagc caataattcg tccgtcaagc agtgttggct
     2701 gataaggagg aaatgtccac gaatcgctcg gacgcacagt atccatatgc gaatttaaca
     2761 ttagcgtagg tttgttttca tcatacgatt tagcaaaaca aaagagattg tttttatggc
     2821 gttgaacatc acaatcatgt tgttttaaaa aattaacaag aaaatcagat ttttcttgtt
     2881 cacttttact aattgacggt ataacaagta atgatgttaa tagttcaatt ggattaataa
     2941 ttattgcttt catatagcta atctgttagt actactgttc cagcatcttt gcctatggct
     3001 gtagcgttgg ttataactac tttactcact cccgcttgta tggcttgaaa agcattttca
     3061 agcttgggta tcatccctcc cttaattatt ccttggtgca catatttgtt aaataatgaa
     3121 ggagtaatta gcggaataac actattatca tccatttcat cggataaaac accatttttc
     3181 tcaaaacaat acactaacgt tacatcaaaa taagtggata atgattttgc caactcacta
     3241 gcaatagtat ctgcatttgt atttagcata gaaccagctc catcatgcgt aagaggagct
     3301 actatgggta ctataccttg ctgtattaat gaggctacta tctgagcatt tacatgcttc
     3361 acatctccta cataaccata gtctacttct tttattgggc gtttaacact ttctataata
     3421 ttcatatctg cccccgttag tcctaacgca tttaccccca atgcttgtag tcctgctaca
     3481 atattcttat taaccagtcc accataaacc attgttacca catccaaggt ctgtttgtct
     3541 gtaattcttc ttccgtctac cattttgctt tctataccta atttctcgct cagtttagta
     3601 gcagagcgtc cacctccatg taccaagact ttatatcctt ctgtatgtgc aaaatctttc
     3661 aaaaaagaag ctaaactagc tgattcctct acaattttac ctcccacttt aattataatc
     3721 aacgactgtt tcatgtttct attctaataa atatagtagt taaaaaaata agcaagcatt
     3781 catctgtttt agtttcttgc ttttttcttg tgcaaatgta aaaaaaagat atgtttattc
     3841 ttatttttac gctatttttg tgcaaattat ttcatgatac catgcaaaaa aggaaaataa
     3901 cagagttaaa tcgcattagt gtagaagaat ttcagtcgaa aactaaactg ccacttgttg
     3961 ttattttgga taatatacga agtctacaca atattggtgc tatttttaga acttgtgatg
     4021 cttttttgat agaagagatt tatctttgtg gtattacagc cacccctcct aatgctgaaa
     4081 ttcataaaac agctttaggt gcagaaaaca cggtgaaatg ggtatatttt gaatcaataa
     4141 tggatgcaat ccgacaatta cagaccaagg gatactccgt atttgccgta gagcaagtaa
     4201 aacaaagcat tcctctcaac gaattgaaaa ttgacgtaac aaaaaaatac gctataattg
     4261 taggcaacga agtaaaggga gtgcaacaag tagctgtaga tatgtgcaac ggtgccattg
     4321 aaataccaca gtttggaaca aagcactcac taaacgtatc cattgcagca ggaatagtta
     4381 tttgggattt ggtaaaagaa ttatctacat cacaatcaga aagaggatga gcttatcggt
     4441 atgtatatat tgtgcatcta gcaatgccat aaactctgtt tattatcaag agacagaaaa
     4501 actagctaga ttattggctc aaaacaacgt taacgttaag tttggaggtg gtagtagcgg
     4561 acttatggga aaaattgcag atgtgtatgt ggaagagaac aatgacaata tagtaggaat
     4621 tatccctaaa ttcatgtgtg aagaaggctg gcaacatcaa caactaaaac agttaattgt
     4681 aacagaaaca atgcacgaga gaaaacaaaa aatgatagaa aatgtagatg cagcaattgc
     4741 tttaccgggt gggattggta cgttagaaga agtgttagag gtactcactt ggaaacaatt
     4801 aggaattttc ttaaaaccta tcttactcgt aaacattaat ggatatttcg atccactact
     4861 tattgcgtta gaacacgctg tgtcagaaaa ctttatgaga aaagaacatt tacaattatt
     4921 taccatcata agttcggtag aaaacatatt gcaggacata caaaaaaacc ctacatatga
     4981 tgtatccata agaaaaattg cacaaattta actatctatg ccagaagact ctttagtaca
     5041 gattacaaat aactatcccc ctttgttgca gagccctgac tctattacaa accatgttcc
     5101 agtgtgggat gatatactct tttcttctac tatgcacata gatagtacgc ggatactgat
     5161 tcccgaagga atggaaagta tatggcgaac aacttcattt tttcattctg atagtgtgat
     5221 tgcattacta ttgagcatat ttattttact gtgtattact attgcatttt ttcgtaaagc
     5281 caaaacacat tattttacaa gtatatttaa acacaaaaac gaaacggaac aaattaatct
     5341 aacagtatta gaacaatgga aacaagttct gtatagtcta tttggattaa ttggatacac
     5401 gagcatagca ctttatttag ttgtagaaaa atctactatc ataaactatt atgaaacatg
     5461 ggaaatagga ggatggcttt ttgttgccct ttttctgtat ttttttatca aatatatctt
     5521 gttgaaactc tatgtgtata aatttttttc
//
LOCUS       DAOPUR010000028         5547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_14621_length_5547_cov_3.049162, whole genome shotgun sequence.
ACCESSION   DAOPUR010000028 DAOPUR010000000
VERSION     DAOPUR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14621_length_5547_cov_3.049162"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PKN90_00840"
     CDS             <1..580
                     /locus_tag="PKN90_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ84863.1"
                     /translation="FYEPVNGNIFYNSHNVKNLSAKSLRQNVGVVMQDGYIFSDTIER
                     NIATGDEEIDSERLQQAVEIAHIKDFIEELPLGLKTKIGASGNGISGGQRQRILIARA
                     VYRNPHYIFFDEATSSLDAKSEKFIHDSLNHFFVGKTVIIIAHRLSTVKSADQIVVLE
                     HGKIIEIGNHEELTHKKGAYYDLVKNQLELGN"
     gene            complement(759..1580)
                     /locus_tag="PKN90_00845"
     CDS             complement(759..1580)
                     /locus_tag="PKN90_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ84864.1"
                     /translation="MNLYRLFCGKLTIKRLINYILLRLEYFISYSSKTPFIHAKPFAA
                     SIEPANYCNLQCPECPTGMGAIQKEATTLPFPLFKKMINPLLPELFYLNLYFQGEPFL
                     NESIYDMIAYANRQKIITNISTNGHFLTDSACQQLITSKLDRIIISLDGATEETYEKY
                     RVGGNFTAVVDGITRLVRLKKEMQSRRPFIELQFIVFSTNEHEIPAIKVLAKSLQVDG
                     LSIKTAQLTNFKKGNPLMPKNTIYSRYKLDKNGSYKRKKPLRNRCWKNWSSIVVS"
     gene            1806..3509
                     /locus_tag="PKN90_00850"
     CDS             1806..3509
                     /locus_tag="PKN90_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ84865.1"
                     /translation="MKFTKTLFRFGAVVLLASVFASCSKMGGLPAEYITVNPNPLEVK
                     ANKVEAEITGTFPEKYFAKKAILEVTPVLRYEGGEAIGTTVIYQGEKVKDNNKVVAYK
                     TGGKFTQNISFDYVPGMKKSKLFLTFKAKIGTKEIQIPEVEIAQGTIVTATLANPLEI
                     AGATGADAFQRVIQEKYEAAILFQIQRAELQKKALTTAEVAALQNKISSIQANDSLKV
                     SSLGLSAYASPDGPTDLNEKLAANRKKNTEDFLTKELKKSKLETTVDAKFIAEDWDGF
                     QELMMQSDIQDKELILRVLSMYNDPAQREKEIKNLSAAYTKIADQILPQLRRSKLELV
                     VDVIGKSDEQILAIAKAEPSKLSLEELLYGASIATSASEKAYITGQAISLFPEDWRAY
                     NNLATLKFADGNVDEAKALLATAVAKGGDQPQVNFNLGLVELTAKNYKKAQEYFGKSA
                     GVGEALAEAQGVAYIQSGEYNKAVTAFGNSTSNNAALANLLAGNYQKATAVVDAVKNP
                     TADTYYLKALIGARSSNKDQVISSLTKAIQADKSYAKKAACDIEFAKYLIDSDFLAIV
                     K"
     gene            complement(3574..4482)
                     /locus_tag="PKN90_00855"
     CDS             complement(3574..4482)
                     /locus_tag="PKN90_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNZ84866.1"
                     /translation="MYRILKLIKEWTLLIAIIFGALFHNFFGYIYSSFPFITPMLIFI
                     MLLLTFSKLEKLSLQIGKLHIKLLTVQLILSILVYAIGSIIHQTLAMGLLICVLVPTA
                     TSASVITNLLKVDIKFITSYLLFSNVVTAFIIPIFLAYAGTNSNENIFFSMYVLAKKV
                     LPTILLPILIVFILKRYLPTMHQRLQHVSGITFYLWSFALTLVIGKTIHIFVTQETSS
                     YSTEIMLALGSLGICIIQFGLGRVIGKKHGERIASGQALGQKNTIFAIWLAQTYLSPL
                     VSIAPAAYVFWQNTINSYQIWKARRN"
     gene            complement(4562..4738)
                     /locus_tag="PKN90_00860"
     CDS             complement(4562..4738)
                     /locus_tag="PKN90_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84867.1"
                     /translation="MDTLIKNLGVIVIILGALALIIPSFLGFNSNTTLSIGGILLFAG
                     LVLHIIMTKKNTEK"
     gene            complement(4820..>5547)
                     /locus_tag="PKN90_00865"
     CDS             complement(4820..>5547)
                     /locus_tag="PKN90_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNZ84868.1"
                     /translation="RDRAKKANLHNKKLRDCRIHYSDDKVGDKATESVIFITEGDSAS
                     GSITKSRDVNTQAVFSLKGKPLNTFGLTKKIVYENEEFNLLQAALNIEDGIEGLRYNK
                     IIIATDADVDGMHIRLLMISFFLQFFPDLIKKGHVYILQTPLFRVRNKKETIYCYSEE
                     ERQEAVNKLGAKAEITRFKGLGEISPDEFKHFIGKDMRLDPVGLRKEDAVKELLAFYM
                     GKNTPERQTFIIDNLIVEEDLEL"
BASE COUNT         1938 a          871 c          961 g         1777 t
ORIGIN      
        1 attttatgag ccagtaaatg gaaatatatt ttacaatagt cataatgtca aaaatttatc
       61 agcaaagagc ttacgtcaaa atgtaggggt cgtaatgcaa gatggctata ttttttcaga
      121 cactattgag cgtaacatag ctacaggtga tgaggaaatt gatagtgaaa gattgcaaca
      181 agctgttgag atagcacata ttaaagattt tattgaggaa ctcccgctcg gacttaaaac
      241 caaaattgga gcatcgggta atggaatttc gggaggtcag aggcaacgaa ttttgatagc
      301 acgtgcagta tatcgaaatc ctcactacat attttttgat gaggctacca gctcgctcga
      361 tgctaaaagt gaaaaattta ttcacgactc attaaatcat ttttttgttg gtaaaactgt
      421 aataattata gcacaccgac tttcgacggt taaaagtgct gatcaaattg ttgttcttga
      481 acatggtaaa ataattgaaa taggtaatca cgaagaacta acccacaaaa aaggagcata
      541 ttatgattta gtaaaaaatc aacttgaatt aggcaattaa acacccattt aatcgaataa
      601 tgcttctaaa tacttttgtt tattctgtgc aattgttgca cataggcaaa gattttctgt
      661 tatgcaaaat ggttgagcga aactttgttg ccttatcgcc attccacaag gagtgaaacc
      721 cattatctaa taagttgcca taagcatata gcccgtgttt atgaaactac gatagaactc
      781 caatttttcc aacatcgatt acgcaaaggc tttttccgtt tataggagcc gtttttgtcc
      841 agtttatatc tactgtatat agtattcttt ggcattagtg gattaccctt tttaaaatta
      901 gttaattgag ccgtcttaat agataagcca tctacctgta gtgattttgc taatacctta
      961 attgctggta tttcgtgttc attcgtacta aaaacaataa actgaagttc aatgaaaggt
     1021 cttcttgatt gcatttcttt ttttaaacgc actaggcgtg taattccatc cacaaccgcc
     1081 gtaaaattac cacccaccct gtatttttcg tatgtttctt ctgttgctcc atcaaggcta
     1141 ataataattc tatcaagttt agatgttatg agttgttggc atgctgaatc tgttaaaaaa
     1201 tgcccattgg tagatatatt ggtaatgatt ttttgcctat ttgcataggc aatcatgtca
     1261 taaatacttt cattcaaaaa aggctctcct tgaaaatata gattgaggta aaatagttca
     1321 ggaagaagag gattaatcat ctttttaaac aaaggaaacg gtagagtagt agcttctttt
     1381 tgtattgctc ccatacctgt agggcattca ggacattgta aattgcagta atttgctggt
     1441 tctatagatg cggcaaaagg ttttgcatgt ataaaaggcg ttttactact atacgagata
     1501 aaatattcta aacgtaaaag aatatagtta attagacgtt ttatcgtaag cttaccacaa
     1561 aaaagccgat ataaattcat atccctcatt atttacagca aaagtcagca ttattatgta
     1621 gaacagcaaa gattttgtat taaatatctt tgagatgagg ttaaaaatac caaaaataga
     1681 attagaaaga ctccattgag ttttacaaca aaattcttta aacaaaaagt tggtattatt
     1741 ttttatatta tgtatctttg ccctaattct gtaaaatgaa tgtataaccc aattaattga
     1801 taagtatgaa attcacaaaa acattatttc gttttggagc tgtagtatta ctggcttccg
     1861 tatttgcatc atgtagtaag atgggcggat tacccgctga gtatatcaca gttaacccca
     1921 atcccctcga agtaaaagca aacaaagtag aggcagaaat tacaggaact tttcctgaaa
     1981 aatattttgc caaaaaagct attttggaag ttacaccagt attgcgttac gaaggtggtg
     2041 aagctatagg tacaacagta atttaccaag gagaaaaagt aaaagataac aacaaagttg
     2101 tggcatacaa aacaggtgga aagtttactc aaaacatttc gtttgattat gtgccaggaa
     2161 tgaaaaaatc aaaattattt ttgactttta aagcaaaaat aggtacaaaa gaaattcaaa
     2221 ttccagaagt tgagattgca caaggtacca ttgtaacagc tacattagca aatccattag
     2281 aaattgctgg agctacaggt gctgatgcat tccaacgtgt tattcaagaa aaatacgaag
     2341 ctgctatttt attccaaatt caacgtgctg aattgcaaaa aaaggcactt actacagcag
     2401 aagttgctgc tttgcaaaac aagatttctt ctattcaagc taatgacagt ttgaaagtat
     2461 cttctttagg tttatctgct tatgcttcgc cagatggtcc aactgacttg aacgaaaaat
     2521 tggctgctaa tcgtaaaaag aatacagaag actttttgac aaaagaattg aaaaagtcaa
     2581 aacttgaaac tacagttgat gcaaaattta ttgcagaaga ctgggatggg tttcaagagt
     2641 tgatgatgca gtctgatatt caagataaag aattaatctt acgtgtatta tctatgtata
     2701 acgacccagc acagagagaa aaagaaatca aaaatctatc tgctgcttat acaaaaattg
     2761 ctgatcaaat tttgccacaa ttacgtcgtt caaaattaga attagtagta gatgttattg
     2821 gtaaatcaga cgagcagatt ttggcaattg ccaaagcaga gccttcaaaa ttgtctcttg
     2881 aggaattgtt gtacggagcg tcaattgcta cttctgcctc tgagaaagca tatattacag
     2941 gacaagcaat ttctttgttc ccagaagatt ggagagcata caataatctt gcaactttga
     3001 aatttgctga tggtaatgtt gatgaagcga aagccttatt agcaactgct gtggcaaaag
     3061 gtggcgacca accacaagtt aatttcaatt taggattagt agaattgaca gcaaagaatt
     3121 acaaaaaagc acaagaatat tttggtaaat cggctggtgt aggcgaagct ttagcagaag
     3181 cacaaggtgt ggcttacatt caatcaggag agtataacaa agcggtaaca gcatttggta
     3241 attctacttc taacaacgct gcattggcta atttattagc aggtaattat caaaaagcga
     3301 cagctgtagt tgatgcagtt aaaaatccaa ctgcggatac atactattta aaagcactta
     3361 ttggtgctcg ttctagtaat aaagatcaag taataagttc attaacaaaa gctattcaag
     3421 cagataaatc gtatgctaag aaagcagctt gtgatattga atttgctaaa tatttaattg
     3481 actctgactt tttggctatt gtaaagtaaa ttttctagtt agatgatgat aaaaaagagg
     3541 ctatccaaaa aggatagcct ctttttatat gattcagttt cgtcgagctt tccaaatttg
     3601 atacgaatta atggtatttt gccaaaaaac atatgctgct ggagcaatag atactaatgg
     3661 cgataggtat gtttgagcaa gccaaatggc aaaaatagta tttttttgtc caagagcctg
     3721 accagaggca atgcgttctc cgtgtttttt tccgataact ctaccaagcc caaattgtat
     3781 tatacatata cctaatgagc ctaaagctag cattatttct gtagaatagg atgaggtttc
     3841 ttgtgtaaca aaaatatgaa tagttttgcc aattaccaac gttaatgcaa acgaccataa
     3901 gtaaaaagta ataccactta catgctgtaa gcgttgatgc atagtgggca aatagcgttt
     3961 tagaataaac acaattagta taggaaggag tatggtaggt aatacttttt tggctaatac
     4021 atacatggag aaaaaaatat tttcgttaga gttagttcct gcatatgcca aaaatatagg
     4081 tataataaaa gccgtaacta cattgctaaa taataaatag ctagtaataa atttgatatc
     4141 tactttcaat aaattggtaa ttacagaagc cgatgttgct gtaggtacta atacgcatat
     4201 aagtaatccc atagccaatg tttgatgtat tatactacca atagcataca caagaatact
     4261 aagaatgagt tgtacagtga gtagtttgat atgcaattta ccaatctgta aacttaattt
     4321 ttctaattta gagaaagtaa gcaatagcat gataaaaata agcataggag taataaatgg
     4381 aaaggaggaa tatatatatc caaagaaatt atgaaacaaa gctccaaaaa ttattgctat
     4441 gagcaatgtc cattctttta ttaattttaa aatacgatac atatgattaa ataaaaaacc
     4501 tttccttata taatctggaa aggtttagtg tacataagaa aacagttaga cagatagtta
     4561 tttacttttc agtatttttt tttgtcatga ttatatgaag gactaaacca gcaaataata
     4621 agattccacc aatactaagt gttgtgttgg aattaaatcc aaggaaagaa ggaataatca
     4681 gagctaaagc tcctaaaata ataacgataa ctcctaggtt tttaattaat gtatccataa
     4741 tgtgtttttt tgtggtatgt tttgttacgc aaataaacga ataattcttg ttgtataaaa
     4801 atatttttca aaaaaaatgc tacaattcta aatcttcttc tactatgagg ttgtcaataa
     4861 taaaagtctg tctttcagga gtatttttgc ccatataaaa agctaaaagc tccttcacgg
     4921 catcttcttt tctaagccca acagggtcta aacgcatgtc cttaccaata aaatgtttaa
     4981 attcatcagg cgatatctct cccaatcctt taaatcgagt aatttcagct ttagctccta
     5041 atttatttac tgcctcttgt ctttcttctt ctgaataaca atagatcgtt tcttttttat
     5101 ttcggactct aaaaagcggt gtttgcaaga tgtatacgtg ccctttttta atcaaatcgg
     5161 gaaagaattg caaaaagaaa ctaatcatta atagtcgaat gtgcatacca tctacgtcgg
     5221 catctgttgc aatgataatt ttattgtatc gcaatccttc aataccatct tcaatattaa
     5281 gagcagcttg caagaggtta aactcttcat tttcgtacac aattttttta gttaaaccaa
     5341 aggtatttaa tggctttcct tttaaactaa aaacggcttg cgtatttaca tcgcgacttt
     5401 tagtaataga gccactagca gaatctcctt cggtaataaa aattacagat tctgttgctt
     5461 tatcaccaac tttatcgtct gaataatgaa tacgacaatc acgtaatttt ttgttatgta
     5521 aattggcttt ttttgccctg tctctag
//
LOCUS       DAOPUR010000029         5539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_14651_length_5539_cov_2.938549, whole genome shotgun sequence.
ACCESSION   DAOPUR010000029 DAOPUR010000000
VERSION     DAOPUR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14651_length_5539_cov_2.938549"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKN90_00870"
     CDS             complement(<1..562)
                     /locus_tag="PKN90_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNZ84869.1"
                     /translation="MYIVALILQRFIFDTWNIPFFITVSLLLALIYLYTYANGIKTIV
                     WTDALQTLFLLIALILLIIQSMDFLSLDINTTVETIKNSSYTRLWIVDDWKNPSHFIK
                     LFISGVFITIAMTGLDQDMMQKNLSCSNTRSAQKNMLSYGMAFIPVNILFLTLGFLLV
                     MVTKTQGIALPAQTDSLLPNIIADGIL"
     gene            complement(638..964)
                     /locus_tag="PKN90_00875"
     CDS             complement(638..964)
                     /locus_tag="PKN90_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84870.1"
                     /translation="MSAIGLLLLIIIYFGVLQCIAFVGRKEKSNTAFFLGNKTSPWYI
                     VSFGMIGASLSGVTFVSVPGWVLSSGFSYMQMVLGFSVGYIIVAFVLLPLYYRLQLTS
                     IYTYLY"
     gene            1050..1676
                     /gene="recR"
                     /locus_tag="PKN90_00880"
     CDS             1050..1676
                     /gene="recR"
                     /locus_tag="PKN90_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNZ84871.1"
                     /translation="MNNHHYPSILLENAVNQFAKLPGIGRKTALRLVLHLLKQEKSEV
                     GGFAQALVSLKNEIRYCAVCKNISDTEVCSICNDTARIRDIVCVVETIKDVMHIENTG
                     QYRGLYHVLGGVISPMDGVGPADIEIESLIQRVQIGEIKEIILALNPTMEGDTTCFYL
                     YKKLLHYPITISTIARGISIGDELQYADEVTLGRSIVHRVPFASSLQL"
     gene            1691..2131
                     /locus_tag="PKN90_00885"
     CDS             1691..2131
                     /locus_tag="PKN90_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84872.1"
                     /translation="MEKEIKTIQKTLLKFYYLLYATLMLILIAGGFLLPKFDPLQAVT
                     VQILNTISIALVLVCIPLGLKYFSDKTKKLKNEVTDKQTRIVSYVRYAKQLMFVLGLP
                     GIFAGVSYYLIPEDKTSLFCFLMSFVALVLSKPTEVKLQKFFIE"
     gene            2169..2591
                     /locus_tag="PKN90_00890"
     CDS             2169..2591
                     /locus_tag="PKN90_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84873.1"
                     /translation="MIIAVDFDGTIVEHAYPSIGDEIPFAIETLKKIQDEGEHQLVLW
                     TVREGELLQEAVEYCRKKGLEFYAVNKDYPEEEYDIRANRKLKADLFIDDRNVGGLPD
                     WGVIYAMIKGKVKPKYDGGYEIPKRKNVFIHIGEWLQK"
     gene            2625..3614
                     /locus_tag="PKN90_00895"
     CDS             2625..3614
                     /locus_tag="PKN90_00895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ84874.1"
                     /translation="MKLSIVIVNYKVPHLLLQCLDAVEKAIQCMDAEVIVVDNNSNDQ
                     SRDLVSTYFSSVIYIANAENVGFSKANNQAIKIAKGEYVLLLNPDTIIAEDTLKTVCD
                     IADTTDRFGALGVRMVDMKGAFLPESKRGIPTVWVAFCKLTFIHKLFPRSTFFNAYYY
                     PNIKENEPGEVPILAGAFMLINKKRLQEEALLDEQFFMYGEDIDLSYRIINQGMKNVY
                     VPTTIVHYKGESTKKGDMKYLKAFYESMLLFYKKHYAGSFPLWFYPISGLIHVYASIA
                     YRCRIKRTRNRRAKETIVLSTNQYSYAEIIQQIEKNKGEKNIQLYHPNFGFSV"
     gene            3614..4141
                     /locus_tag="PKN90_00900"
     CDS             3614..4141
                     /locus_tag="PKN90_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ84875.1"
                     /translation="MNNELLHNTIITLRAIEPEDLDLFYKWENDTMYWEHGSTIVPFS
                     RYALKQYIQATTQDVYETKQLRLMIEHVRDKVCLGTIDVSQIDFFHKRAEMGVFIDKH
                     YQQKGFAVMAIQLLLEYLFSFLDLEQVYCHVPKNHISSQKMLLKTGFVCVGEKKHWIR
                     KGNEFEDVLFFQKLK"
     gene            complement(4153..4938)
                     /locus_tag="PKN90_00905"
     CDS             complement(4153..4938)
                     /locus_tag="PKN90_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNZ84876.1"
                     /translation="MIQDNLTVSIIQTDITWENKAKNLEMFAEKVRACGSYSDIILLP
                     EMFTTGFSMHASNLAETVHDETINTLKKWALTENAMLVGSFIAIDNNHVYNRGFAVLP
                     SQEIYWYDKKHLFSMSKEDAVFTPGNAPCIVPYKGWNISLAICYDLRFPVWLRNTNNA
                     YDMLLVVANWPESRKEAFNTLLKARAIENSCYVCAANRIGIDGNQVVYAGNSQIVDFK
                     GTIVANTAEHTDNIVSATVNKDLLIAFREKFSVWKDADPFSLL"
     gene            complement(4935..5498)
                     /locus_tag="PKN90_00910"
     CDS             complement(4935..5498)
                     /locus_tag="PKN90_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ84877.1"
                     /translation="MKKNISKMLLKLMGWKTTASIPSIKKLVICVAPHTSNWDFIIGK
                     LFYTALGGKASFLIKKEWLKPPVGKLLINMGAIGVNRGKNTSLTDQLAKVFQEKEEFH
                     LAITPEGTRKKIDKWKLGFYQIAKKAGVPIAMMTIDYKHKEVSVLGIYEPTDDEEKDI
                     NMIKSFYENAHAKYPSQFSLGKDYKQV"
BASE COUNT         1864 a          855 c         1019 g         1801 t
ORIGIN      
        1 ccaatatgcc atcggctatg atgtttggca gtaaagagtc tgtttgagct ggtagtgcaa
       61 taccttgtgt tttagttacc attaccaata aaaaacctaa agttaaaaac aatatattaa
      121 ccggtataaa agccatacca taactgagca tatttttttg agcacttcta gtatttgagc
      181 aagatagatt tttttgcatc atatcttggt caagtcctgt catggcaata gtaataaata
      241 caccgctgat aaataatttg ataaaatgag acggattttt ccaatcatca actatccata
      301 agcgagtgta agagctattt ttaattgtct caactgttgt gtttatatcc aaagataaaa
      361 aatccatcga ttgaataata agtaaaataa gggcaatgag taaaaataag gtttgtaaag
      421 catctgtcca aacaatagtt ttaatgccgt ttgcgtaagt ataaagataa atcaaagcaa
      481 gtaataaact tactgttata aaaaaaggta tattccatgt gtcaaaaata aaacgttgta
      541 aaataagagc aacgatatac aaacgtgtag ctccaccaat agtgcgtgat aataagaaaa
      601 ataatgagcc tgttgtgtgt gtcgctttgc caaaacgtta gtataagtat gtatagatac
      661 ttgtaagttg caagcggtaa tataaaggaa gtaaaacaaa agccacaata atatagccca
      721 ccgaaaagcc aagtaccatt tgcatataac taaatcctga tgatagtacc caaccgggca
      781 cagatacaaa cgtaaccccc gaaagggatg ccccaatcat gccaaatgaa acaatatacc
      841 aaggcgaagt tttgttgcct aaaaaaaatg cagtattgct cttctctttc cttccaacaa
      901 aagcaataca ttgcaatacc ccaaagtata taataattaa aagaagtaat ccaattgctg
      961 acatgttttt actaaattag accacaaagg tattaaaaaa taacaaaaaa gtgatatctt
     1021 tgtagtttga ttagtaagta aatgcttgta tgaataatca tcattaccca tctatattgc
     1081 tcgaaaatgc ggttaatcag tttgctaaat tacctggcat aggtaggaaa acagctctcc
     1141 gattagtatt acatttatta aagcaagaaa aatccgaagt gggtggtttt gcacaggcat
     1201 tagtttcttt gaaaaatgaa atacggtatt gtgccgtatg caaaaatata tcagatacag
     1261 aggtatgtag tatctgtaat gatacggctc gtatacgtga tattgtgtgt gtggtagaaa
     1321 caattaaaga cgtgatgcat atcgaaaata caggtcaata tagaggcttg tatcacgtgt
     1381 tgggaggagt tatttccccg atggatggcg ttggtcctgc tgatattgaa atagagagtt
     1441 tgattcaacg ggtacaaata ggcgaaatta aagaaattat tttagcgtta aatccaacca
     1501 tggaagggga tactacgtgt ttttatttat acaagaaatt actccattat cctatcacta
     1561 ttagcactat tgcacgtggt atttcaatag gagacgaatt gcaatatgcc gatgaagtaa
     1621 cattgggcag atctattgtg catcgtgttc catttgcatc ttcattacaa ctataatttt
     1681 aatacaatat atggaaaaag aaataaaaac aatacagaaa acgttgctaa agttttatta
     1741 tctactgtat gccacattga tgcttatatt gatagctggt ggatttttgc tacccaagtt
     1801 tgacccatta caggctgtta cagtacaaat actaaacaca atttctattg ccttggtgtt
     1861 ggtatgtata cccttaggat taaagtattt ttcggataaa acaaaaaagt taaaaaacga
     1921 agtaactgat aagcaaacac gtattgtgag ctatgttcgc tatgctaaac agcttatgtt
     1981 tgtgctaggt ttgccaggca tatttgctgg tgtatcgtat tatctgatac cagaagataa
     2041 aacttccttg ttttgttttt taatgtcgtt tgtagcatta gtcctttcta aacctactga
     2101 agttaaattg caaaagtttt ttatagaata aatgtaagtt tggtacctag tatatataac
     2161 aaatacctat gataatagct gttgattttg atggaactat tgttgagcat gcctatccaa
     2221 gcattggtga tgagatacca tttgccatcg aaaccctaaa aaaaattcaa gacgaaggag
     2281 agcaccagtt ggtgctttgg acagtacgag aaggagaatt attgcaagag gctgtggagt
     2341 attgtcgaaa aaaaggctta gaattttatg ccgttaataa agattatcca gaagaagaat
     2401 acgacataag agccaatcgt aagctaaagg ctgatttatt tattgatgat agaaatgtgg
     2461 gaggactacc tgattggggg gtaatatatg ccatgattaa agggaaagta aaacctaaat
     2521 acgatggtgg ttatgaaatt ccaaaacgaa aaaacgtatt tatccatatc ggagagtggt
     2581 tacagaaata gaagtagtga gttgttgata tttattttta atgtatgaag ttgtctattg
     2641 tcattgttaa ttataaggtg ccccatttgc tgttgcaatg tttagatgct gtagaaaaag
     2701 caattcaatg tatggatgct gaggtaattg tggtggacaa taattccaat gaccaatcac
     2761 gcgatttagt ctctacatat ttctcttctg ttatttacat agctaatgcc gaaaatgttg
     2821 gtttttcaaa agcaaacaat caggcgataa aaatagcaaa aggagagtat gttcttttgc
     2881 tcaatccaga tactattata gctgaggata cattgaaaac tgtatgcgat atagcggata
     2941 ctaccgatcg tttcggagca ttaggtgtac gaatggtaga tatgaaaggg gcttttttgc
     3001 ctgaatcgaa aagaggtatt cctacagtgt gggtagcttt ttgtaagtta acatttatac
     3061 ataaattatt cccacgtagt acctttttta atgcgtatta ttatcccaat atcaaagaaa
     3121 acgagcctgg agaggtgcct atattagcag gtgcattcat gttaataaat aaaaaaagat
     3181 tacaagaaga ggctttgtta gatgaacagt tttttatgta tggagaagat attgatttat
     3241 cctacaggat tatcaatcaa gggatgaaaa atgtctatgt tcccacaact attgttcatt
     3301 ataaaggaga aagtacaaag aaaggggata tgaagtacct aaaagcattt tatgagtcga
     3361 tgttgttgtt ttataaaaaa cattatgcag ggtcttttcc cttgtggttt tatcctattt
     3421 cgggtttgat acatgtgtat gccagcatag cttatcgttg tcgtataaaa cgcactcgta
     3481 acagaagagc aaaagaaaca attgtgttat ctactaatca gtattcatat gctgagatta
     3541 ttcaacaaat tgaaaaaaat aaaggagaaa aaaatattca actatatcat cctaattttg
     3601 gtttttctgt ataatgaaca atgagttgct ccataatacg ataattacac ttagagccat
     3661 tgagcctgag gatcttgacc ttttttataa gtgggaaaat gatactatgt attgggaaca
     3721 tgggagtact attgtgccat tttctcgtta tgccttaaaa caatatatac aagccacaac
     3781 gcaagacgtg tatgaaacaa agcagttgcg tttaatgatt gaacatgtaa gggataaagt
     3841 ttgtttagga accatagatg ttagccaaat tgattttttt cacaaacgtg ctgaaatggg
     3901 tgtatttata gataaacact atcagcagaa aggttttgct gtaatggcaa tacaactatt
     3961 attggagtat cttttttctt ttctcgattt ggaacaagtg tattgtcatg tgccaaaaaa
     4021 ccatatttct tctcaaaaaa tgcttttaaa aacaggattt gtgtgtgtcg gtgaaaaaaa
     4081 acactggata agaaaaggca atgaatttga ggatgtattg ttttttcaaa aacttaagta
     4141 atgcatttgt atttacaaaa ggctaaatgg gtcagcatct ttccaaacag aaaatttttc
     4201 cctgaaagca attaacaagt ctttgttaac agtagctgaa acaatgttgt ccgtatgctc
     4261 tgctgtattt gctactatag tgcctttaaa atctactatt tgcgaattgc ccgcataaac
     4321 cacttgattt ccatcaatgc caatacgatt agcagcacat acatagcaac tattttcaat
     4381 agcacgagct tttagtaagg tattaaaagc ttcttttctg ctttcgggcc aattggctac
     4441 taccaatagc atatcatagg cattatttgt attacgtagc caaacgggga aacgtaaatc
     4501 ataacagata gctaatgaaa tattccatcc tttgtatggt acaatacatg gtgcatttcc
     4561 aggggtaaaa acggcatctt ctttgctcat agaaaataaa tgctttttgt cgtaccaata
     4621 tatttcttgt gatggtaaaa cggcgaaacc tctgttgtac acatgattat tgtctatggc
     4681 gataaaactt ccaactagca ttgcattttc tgttaatgcc catttcttta aggtgttaat
     4741 tgtctcatca tgtactgttt cagctaggtt actagcatgc atactgaacc ctgtcgtaaa
     4801 catttcgggt aatagtatga tgtcggagta agacccacat gctcgaactt tttctgcaaa
     4861 catttcaagg ttttttgctt tattttccca tgtaatgtct gtttggataa tgctaactgt
     4921 aagattgtct tgtatcatac ttgtttataa tctttaccta aagaaaattg agagggatat
     4981 ttggcatggg cattttcgta aaacgatttt atcatgttaa tgtctttttc ttcatcatca
     5041 gtgggttcgt aaatgcctaa tacagaaact tctttatgtt tataatcaat tgtcatcatg
     5101 gcaataggta ctcctgcttt ttttgcaatt tgataaaatc ccaatttcca cttatctatt
     5161 ttttttcgtg ttccttcagg ggtaattgct aaatgaaatt cttccttttc ttgaaaaact
     5221 ttagctaatt ggtctgtgag cgaagtgttt ttcccccggt taacgccaat ggctcccata
     5281 ttgattagta attttcctac aggaggtttc agccattctt ttttgataag aaaagaagct
     5341 tttcccccta gagctgtata aaaaagtttt ccaataataa aatcccaatt actagtatgt
     5401 ggagcaacgc aaatcactaa ttttttgata gagggaatgc tggctgttgt tttccatccc
     5461 attaatttta acaacatttt gctgatattt tttttcataa aataaagata aaaataacaa
     5521 ttaaaacctt cttgaagta
//
LOCUS       DAOPUR010000030         5422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_15081_length_5422_cov_3.014533, whole genome shotgun sequence.
ACCESSION   DAOPUR010000030 DAOPUR010000000
VERSION     DAOPUR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15081_length_5422_cov_3.014533"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /gene="topA"
                     /locus_tag="PKN90_00915"
     CDS             complement(<1..1156)
                     /gene="topA"
                     /locus_tag="PKN90_00915"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNZ84878.1"
                     /translation="MGKNIVIVESPAKAKTIEKFLGKDYHVVSSFGHIRDLKKKDISI
                     DFNNNYSPIYEVPTDKKKLVSELKKKASEAEMVWLASDEDREGEAIAWHLFEVLSLTE
                     DKTKRIVFHEITKDAIINAIQTPRKIDKALVDAQQARRVLDRIVGFELSPILWKKIKP
                     SLSAGRVQSVAVRLIVEREREITTFKSEANYRITAVFITKDTQGKTVELQAELPKRFK
                     TKEEANRFLEVCKDAQFNIEDISKKPSKKSPAAPFTTSTLQQEAARKLGYSVAQTMMV
                     AQKLYENGLITYMRTDSVNLSALAINTAKAEIIHFAGEQYVKTRQYATKSKGAQEAHE
                     AIRPTYMNQHTIEGNRQEKFLYELIWKRTIASQMADAEIEKTTVTIGISTS"
     gene            1368..1793
                     /locus_tag="PKN90_00920"
     CDS             1368..1793
                     /locus_tag="PKN90_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNZ84879.1"
                     /translation="MDKSIISLQQLIANSGMKVTPQRLAVFEAVMSMRTHPTADAIIA
                     RVQEQHPHIAVGTIYNVLDALVDKKLIVRVKTDKDSMRYDAVLDDHHHLYCTQTGEIV
                     DYYNQELSLILTNYFKERGIPNFEIQDIKLQINGIYKPQ"
     gene            1815..2528
                     /locus_tag="PKN90_00925"
     CDS             1815..2528
                     /locus_tag="PKN90_00925"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNZ84880.1"
                     /translation="MENNQEVYAMPRIGEKAPVFKAVTTQGEINFPADYSGSWVILFS
                     HPADFTPVCTSEFMTFATMEKQFNQANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMK
                     DVEVTFPLIEDITMEVAKKYGMMMPGESNTKAVRAVFVIDPQGIIRTIIYYPLSLGRN
                     FDELYRVILALQTADEFNVATPADWRPGDDVIISPAGSCGTAKDRMDGKEDGLECKDW
                     FFCTKKLDKDLVLGKILKK"
     gene            2821..5241
                     /locus_tag="PKN90_00930"
     CDS             2821..5241
                     /locus_tag="PKN90_00930"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNZ84881.1"
                     /translation="MKKQLSVLLFLLCVWSVVLAQHRKEFTIVQTSIPPKIDAVPTDT
                     VWDAAYSIRDFIQQSPTNGGVPSQHTEVRMLYDNEAIYVLAMLYDTKPDSIYAEIGDR
                     DSGDEANADLFSVEINPYNDGLNATEFMVSAAGVQMDSKNDLQTMNKSWDAVWKSEVK
                     KTNIGWIVEMKIPFSALRFPNTNTQVWEINFFRLIKRNGEGITWNFVDKNKNGWLNQA
                     GEMHGIRGINPPLRLSFMPYIATYYKDNKFLIKGGLDVKVGLNESFTLDMMLIPDFGQ
                     TRADDTYLNLSSVETKYDEKRQFFTEGTELFSKGDIFYSRRIGGQPRYYNKVYSYTKE
                     NEILQRNPSETNLYNATKISGHTSSGWGIGMLNAITKKQVAEIKDTLTDVTRYVTTQG
                     VSNYNVMVVNKVIGKESYISWVNTNLLIPEQNYVSNVSGFDFKYMLKDHAFTGDAFVS
                     YNKERELSPISNELGYRANVTISKLKGTFRYEYLATILSDSYNPTDIGYLENNNIITN
                     TLILYYLNYTPTKSVNEVTSQLAIYYDNLYHPLLYSRLELAWNLKLLFPSFNSIKFYI
                     SATPIEKYDHFEPRVVGWKAKEPTAGYVGAEYSSDYRKKLAFFTNIGAWKASQYNKSA
                     QYFTLAPTYRINDKLALTYQFISSFLQNAIGYVDKNNTNDSIFFGRRDISNIENIAQV
                     KYSLSNNSLLNLRLRHYWSNVDHHAYYLLQHDGTFQSTIREANYNTNVSALNADISYT
                     WRFLPGSELSLVWKRYFYLYNQDVASSYVKNLQNVLDYSQVNSVSLKLIYYIDYQTLK
                     TAKKAIFL"
BASE COUNT         1706 a          856 c         1039 g         1821 t
ORIGIN      
        1 cgctagtaga gataccaata gttacagttg ttttttctat ttcagcatca gccatttgcg
       61 atgcaattgt acgtttccaa atcaactcat acaagaattt ttcttgtcga tttccttcta
      121 tggtatgttg attcatgtag gtaggacgta tggcttcgtg tgcttcttgt gctcctttcg
      181 atttggtagc gtattggcga gtttttacgt attgctctcc agcaaaatgt ataatttccg
      241 ctttagctgt attaatagcc aaggcagaaa ggtttacaga atcggtacgc atatacgtaa
      301 taagtccatt ttcgtacaat ttttgtgcta ccatcatggt ttgtgctacc gagtagccaa
      361 gttttctggc agcttcttgc tgcaatgtag atgttgtaaa tggggcagca ggcgattttt
      421 tgctcggttt tttgcttatg tcttcaatgt tgaattgagc gtctttgcac acttctaaaa
      481 agcggtttgc ttcttctttg gttttaaaac gtttgggtaa ttcggcttgt aactctaccg
      541 tttttccttg tgtatctttg gtaataaaca cagcagtaat gcggtaattt gcttcagatt
      601 tgaaagtagt tatttctctc tctcgttcta cgattaaacg aacagctact gattgtacac
      661 gaccagcact caacgaaggt ttaatttttt tccacaatat gggcgaaagt tcaaaaccta
      721 caatgcgatc gagcacccta cgtgcttgtt gtgcatctac cagtgctttg tctatttttc
      781 tgggtgtttg tatggcattg ataattgcgt ctttggtaat ttcgtgaaat acaattcgtt
      841 tggttttgtc ttcggtcaac gataatactt caaataaatg ccaagcaatg gcttctccct
      901 cgcggtcttc atcggatgcc aaccatacca tctcggcttc agaggctttc ttttttaatt
      961 cgcttaccag tttctttttg tcggttggaa cctcgtaaat aggggagtaa ttattgttaa
     1021 aatctatgct aatgtctttt ttcttcaaat cgcgtatatg accaaaacta gacacaacat
     1081 ggtaatcttt tccaagaaat ttttcaatcg ttttggcttt tgccggagat tccacaataa
     1141 caatattctt tcccattttt gtaccaagtt agcagattaa ttaacaaaat tcggtgcaaa
     1201 agtaataaaa ttgatattga taaaactatt ttttcgatat aaatagttgt tttttatggt
     1261 ttttacagaa aagtgtgaaa attaatgcaa aaatatttgg ttaattgaaa aatatagatt
     1321 aattttgttc ttaaatagga attattccta ataaataata agtataagtg gataaatcaa
     1381 taatttcatt acaacaactc atcgcaaata gcgggatgaa agtaactccc cagcgattag
     1441 ccgtattcga agctgttatg tctatgagga ctcaccccac agctgatgcc attattgcac
     1501 gtgttcaaga gcagcatcct catatagcag tgggcactat atataatgtg ttagatgcct
     1561 tggtagataa aaaattgatt gtacgggtaa aaaccgacaa agatagcatg cggtatgatg
     1621 ctgttttgga cgatcatcat cacttgtatt gtacgcaaac aggagaaata gttgattatt
     1681 ataatcaaga attaagtttg atacttacaa attattttaa agaaagaggg atacctaatt
     1741 ttgaaataca agatattaaa ttacaaataa acggaatata taaaccacaa taataataaa
     1801 aaaacaaaaa gattatggaa aacaatcaag aagtatatgc tatgccacgt attggcgaaa
     1861 aagctcccgt ttttaaggct gttactaccc aaggtgaaat taatttccct gctgattata
     1921 gcggtagttg ggtaatttta tttagtcatc cagccgattt tactcctgta tgtacgtcag
     1981 aatttatgac atttgctact atggaaaaac agtttaatca ggcaaattgt aagttggtag
     2041 gactttcagt cgatggtttg tatagtcata ttgcatggct acgtactatc aaagaaaaaa
     2101 ttgagtataa agggatgaaa gatgtagagg taacttttcc tcttattgaa gatattacga
     2161 tggaagttgc caaaaaatat ggcatgatga tgcctggcga aagcaataca aaagcggtaa
     2221 gagctgtatt tgtaattgac ccacagggaa ttatccgaac aattatttat tacccattga
     2281 gcttaggacg caactttgat gagttgtata gagtgatatt ggcattgcaa acagctgatg
     2341 agtttaatgt agcgactcct gctgattggc gtccaggtga tgatgtgatt atttcaccag
     2401 caggttcgtg tggtactgcc aaagatagaa tggatggtaa agaagatgga ctggaatgta
     2461 aagactggtt cttctgtacc aaaaaacttg acaaagacct tgtgttgggt aaaattctta
     2521 aaaaatagta tttcaacttt cctaaaaagg atagagctac tctgtatgag tggctctttt
     2581 tttgcataaa agagagttgt aagctaaagt atgatcaaat tatacaatat gttgcaacga
     2641 tcttttatgt tggttgcgtg ttatagttat ccttttttct atattcttgt gtcagaagat
     2701 tatcataaag agtatttttt tctacgtttg cataaatagt agtacgttgg tgtaatattt
     2761 tttttgttgt tagtttattc tattttcttt gggatgataa ttatacctta tgttacgtct
     2821 atgaaaaaac aacttagtgt gctactgttt ttattgtgtg tatggtcagt tgtattggct
     2881 caacacagaa aagagtttac cattgtgcaa acaagtatcc cgcctaaaat tgatgctgtt
     2941 cctaccgata ctgtatggga tgcagcatat agtattcgcg attttattca gcagagtcct
     3001 actaacggtg gtgtgccttc gcaacacaca gaggtaagaa tgctatatga taatgaggca
     3061 atatatgtgt tggcaatgct gtatgacact aagcctgatt ctatttatgc tgaaataggg
     3121 gatagggatt cgggcgatga ggctaatgcc gaccttttct cggtagagat aaacccctat
     3181 aacgatggac tcaatgctac tgaatttatg gtttcggcgg caggagtcca gatggattcg
     3241 aaaaacgatt tacagaccat gaacaaaagt tgggatgctg tgtggaaaag tgaagttaag
     3301 aaaaccaaca tcggttggat agtggaaatg aaaattcctt tttctgcatt acgttttcct
     3361 aatacaaata cacaggtttg ggaaattaat ttttttcgtc tgattaaacg taatggagag
     3421 ggtataacat ggaattttgt cgataaaaat aaaaacggct ggctgaatca agctggcgag
     3481 atgcacggca ttcgtggtat taatcctcca ttacggcttt cgtttatgcc ctatattgct
     3541 acatactata aagataataa gtttttgata aaaggaggat tggatgtaaa agtgggactc
     3601 aatgaaagtt ttactctcga tatgatgctc attcccgatt ttgggcaaac acgagccgat
     3661 gatacgtatt taaacttgtc gtcagtggaa acaaagtatg atgaaaaacg acaatttttt
     3721 actgagggta ccgagctatt tagcaaaggc gatatatttt attcacgtcg aataggaggt
     3781 caacctcgct actataacaa ggtatattca tatacaaagg aaaatgaaat actacaaaga
     3841 aacccatctg aaacgaattt atataatgca acgaaaattt ctggacatac ttcttctggt
     3901 tggggaatag gtatgttaaa tgctattaca aaaaaacaag tagctgaaat aaaagatact
     3961 cttaccgatg ttacaagata tgtaactaca caaggggtat ccaattacaa tgtgatggta
     4021 gtaaataaag ttattgggaa agaatcgtat attagttggg tcaatacaaa tttgcttatt
     4081 cctgagcaaa attatgtgtc gaatgtgtcc ggttttgatt ttaaatatat gttgaaagat
     4141 catgcgttta ctggagatgc atttgtgagt tataataagg aaagagagct ttctcctata
     4201 tccaacgagc tgggttatag ggcaaatgtt actatctcaa aactgaaagg gacctttaga
     4261 tacgaatacc tcgcaactat tttatccgat tcatataatc ctacggatat agggtatctt
     4321 gaaaataata atatcattac gaatacgtta attttgtatt atttaaatta tactcctacc
     4381 aaatcagtaa atgaagttac tagtcaactg gctatttatt acgataatct ttatcatcca
     4441 ttattgtata gtagattaga gttggcatgg aatttaaaat tgttatttcc atcatttaat
     4501 tcaataaagt tttatattag tgcaacacct atcgaaaagt acgaccattt tgaaccacgt
     4561 gttgtaggtt ggaaagcaaa agaacctacc gctggctatg tgggagcaga gtattcttct
     4621 gattatcgta aaaaattagc tttttttaca aatataggtg cttggaaagc ctctcaatac
     4681 aataaaagtg cccaatattt tacattggct cctacgtatc gcattaatga taagcttgca
     4741 ctcacgtatc agtttataag tagttttttg caaaatgcta tagggtatgt tgataaaaat
     4801 aacaccaatg attcaatctt ttttggcaga agagatattt caaatattga gaatattgca
     4861 caagtcaaat attctttgtc aaacaatagt ttgctaaatc ttcgattacg tcattattgg
     4921 tcaaatgtag accatcatgc atattatctt ttacagcacg atggcacatt tcagtcaact
     4981 atcagggagg ccaattataa taccaatgtt agtgcattaa atgctgacat aagttacacg
     5041 tggcgttttt tgcccggaag cgaactatca ttggtttgga aaagatattt ttatctgtac
     5101 aaccaagatg ttgctagtag ttatgtcaaa aatttgcaaa acgtattaga ttactctcaa
     5161 gtaaatagtg tttcactaaa attgatttac tatatcgatt atcaaactct aaaaacagca
     5221 aagaaagcaa tttttctgtg attattgcct ctaatataat ggaaggatct tatcttttcc
     5281 tcaagcaatt aattttttta cgcgtgtttg tttattcact aaaatacgtt cgtaatgtac
     5341 agcgacttct tttacaacat cttcccaaaa acgggataaa gtttgggagg ctttttctcc
     5401 aacttcctgt agtatgtgtt tg
//
LOCUS       DAOPUR010000031         5309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_15510_length_5309_cov_3.526266, whole genome shotgun sequence.
ACCESSION   DAOPUR010000031 DAOPUR010000000
VERSION     DAOPUR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15510_length_5309_cov_3.526266"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /gene="rfbA"
                     /locus_tag="PKN90_00935"
     CDS             complement(<1..655)
                     /gene="rfbA"
                     /locus_tag="PKN90_00935"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNZ84882.1"
                     /translation="MKGIVLAGGSGTRLYPITKSISKQIIPVYDKPMIYYPISVLMLA
                     GIREILIISTPQDIHLYENLLGDGTSLGVKFTYAIQPSPDGLAQAFIIGDEFIGNDSV
                     CMVLGDNIFYGYNFSKQLKEAATLEDGAVVFGYYVNDPERYGVAEFDTNGKVLSLEEK
                     PAIPKSNYAVTGLYFYSNDVIQKAKGLKPSARGELEITDLNKLYLQEERLKVQLLGRG
                     "
     gene            complement(717..1247)
                     /locus_tag="PKN90_00940"
     CDS             complement(717..1247)
                     /locus_tag="PKN90_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HNZ84883.1"
                     /translation="MIIARQKRKENIAEYILYMWHIEDIIRAYKLDIEAIHTHIITHF
                     SLDETTRKEMKEWYESLIEMMNKEQVVHTGHLQINQSSVRLLCDFHVELLENEDEKQY
                     HSLYHQTLPLIEEFRHKNALSDVPSIEVCLQFMYGIWLLKIQKKEISTETNQAVVQIG
                     KLLAFLSKKFKNNEEN"
     gene            complement(1261..1656)
                     /locus_tag="PKN90_00945"
     CDS             complement(1261..1656)
                     /locus_tag="PKN90_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNZ84884.1"
                     /translation="MIKEEVRIDKWLWAVRLFKTRTLAAEACKKGKITIDTVSVKPSR
                     TIRVNDVIQIKKPPITYAFKVLAVTENRMGAKLVPSFMENVTSEEQYAILELSKISGF
                     IDRDKGTGRPTKKDRRSLESFLDTDWTEE"
     gene            complement(1695..2258)
                     /gene="pth"
                     /locus_tag="PKN90_00950"
     CDS             complement(1695..2258)
                     /gene="pth"
                     /locus_tag="PKN90_00950"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNZ84885.1"
                     /translation="MKYLIVGLGNIGSEYQDTRHNIGFTLLDAFAKASNVFFTEKRYG
                     AVCECKIKNKTMILLKPSTFMNLSGNAVRYWMKEEKIPIENVLILVDDLALPFGVLRL
                     KSKGSDAGHNGLKNIQEILGTDQYPRLRFGIGNNYPKGQQINYVLGKWTLEENTALPS
                     RMQIAIDIIQSFVLAGIQHTMNLYNNK"
     gene            complement(2265..2840)
                     /locus_tag="PKN90_00955"
     CDS             complement(2265..2840)
                     /locus_tag="PKN90_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNZ84886.1"
                     /translation="MKTFELSGVTRTDLGKKPSKAVRNDNAIPCVMYGTGSNTHFTVT
                     ESDVRKLIYTPEIFEVHLTVDGKKSVAVMKDLQFHPVTDKVLHIDFLEVDNKKPIVLS
                     VPVKVEGLAEGVKAGGKLSLELRKLKVKALCKDIPENLVVNVEKLGLGKTLKAGQLSY
                     PNLEILNNKDNVIVAVKLTRAARGLAAKGEK"
     gene            complement(2976..4019)
                     /gene="thiL"
                     /locus_tag="PKN90_00960"
     CDS             complement(2976..4019)
                     /gene="thiL"
                     /locus_tag="PKN90_00960"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNZ84887.1"
                     /translation="MQTRTTISELGEFGLIERLASTISLQHPTSIKGIGDDAAVIENN
                     EGNSQTLISTDLLLEGIHFDLTYFPLKHLGYKAAIVNFSDIYAMNGTPQQITVSIGVS
                     NRFCIEDIEELYEGIKLACSTYKVDIIGGDTSASLTGLTISITCIGVVNTKTIAYRNT
                     AKEGDLICVSGNLGAAYMGLQLLEREKTLFRQTNNAQPDFTGKEYILQRQLKPEARKD
                     IIELLAKESIIPTSMIDISDGLSSETLHICTQSKVGCRIYEDKIPIDYQTASMANEFN
                     MNLVTAALNGGEDYELLFTVPLSMHEKIATLEGISIIGHTTKESLGCNLITRDGQEFQ
                     LKAQGWNTLHKND"
     gene            4192..5085
                     /gene="gldA"
                     /locus_tag="PKN90_00965"
     CDS             4192..5085
                     /gene="gldA"
                     /locus_tag="PKN90_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HNZ84888.1"
                     /translation="MSLYIDSLSKFYGTQHVLKNISFHVESGEIIGFLGPNGAGKSTA
                     MKIITGFVQPDSGSIFVDGIDVLNDTLHAQKIIGYLPEHNPLYLDMYVAEYLLFIAQL
                     YKLGKESKQRVEDIIEKTGLTREYTKKIGQLSKGYRQRVGLAQAMIHNPKVLILDEPT
                     TGLDPNQLVEIRELIKEVGKDKTVILSTHIMQEVQAVCDRYVIIREGSIVADSKKDKI
                     HDTMTIVVEFANPISKEHLFMIDKITAIHAINARSYVVECNEDIRYLLYQFAVEQGVG
                     LVTLHLQEQNLEELFRKLTRN"
     gene            5111..>5309
                     /locus_tag="PKN90_00970"
     CDS             5111..>5309
                     /locus_tag="PKN90_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNZ84889.1"
                     /translation="MEDNFDIRKGALTDKEQDVDIALRPLSFNDFSGQKSIVDNLKIF
                     VQAARMRGESLDHVLLHGPPGL"
BASE COUNT         1594 a          898 c          910 g         1907 t
ORIGIN      
        1 tgccccgtcc aagtagttgt acttttaggc gttcttcttg caagtaaagt ttgtttaagt
       61 cggtaatttc cagttcgcca cgtgccgatg gttttaatcc ttttgctttt tgaatcacat
      121 cgttgctata aaaatacaag ccagtaacgg cataattaga ttttggtatc gctggttttt
      181 cttctaaact taaaactttg ccattggtgt caaactcagc tactccatag cgttcggggt
      241 catttacata ataaccgaat actacagctc catcttcgag tgttgcagct tcttttagtt
      301 gttttgaaaa attatatcca taaaatatgt tatctcccaa taccatacat actgagtcgt
      361 tgccaataaa ttcgtcgcca ataataaaag cttgtgctag cccatctggt gagggctgaa
      421 tagcataggt aaattttaca cctaaactag taccatcacc taataagttt tcgtataaat
      481 ggatatcttg aggagtagaa ataattagaa tttctctaat gcctgcaagc attaaaacag
      541 agattggata atatatcatc ggtttgtcat aaaccggaat aatttgtttt gaaatacttt
      601 ttgtaattgg atacaaacgt gttccagagc ctccagcaag tacaattcct ttcatatgga
      661 ttttttttat ttattagtgt aaatgaattt accgcaccaa agttacaagt attttgttaa
      721 ttctcctcat tgtttttaaa tttcttagac aaaaatgcta aaagtttacc aatttgtact
      781 actgcttggt tggtttcggt agaaatctct tttttctgaa tttttagcaa ccaaatgcca
      841 tacataaatt gcaagcagac ttcgattgaa gggacatcgc ttagtgcatt tttatgtctg
      901 aattcttcga ttaatggtag cgtttgatga tataatgaat gatactgttt ttcgtcttca
      961 ttttcgagca attctacatg aaaatcacaa agcaaacgaa cggaactctg gttgatttgc
     1021 aagtgtcctg tatgcaccac ttgttcctta ttcatcattt ctattagact ttcgtaccat
     1081 tctttcattt cttttcttgt ggtttcatca aggctaaaat gcgttatgat atgtgtgtgt
     1141 attgcttcaa tatctaactt gtatgctctg ataatatctt caatatgcca catatacaaa
     1201 atgtattcag caatattttc ttttcttttt tgacgggcaa taatcatacg ttttcttttt
     1261 ttactcttct gtccaatcgg tatctaagaa agattctaac gaacgtctgt ctttttttgt
     1321 tggtcgtcca gttcctttgt ctctatcgat aaagcctgaa atctttgata attctaaaat
     1381 agcatattgt tcttcggatg ttacattttc cataaaggag ggtactaact ttgcacccat
     1441 tctgttttct gttaccgcta ataccttaaa agcatacgtg atagggggct tttttatttg
     1501 tattacatcg tttacacgga tagtacgtga tggtttgaca gatacagtat caatggtaat
     1561 ttttcctttt ttacaggctt cagcagcaag tgtacgggtt ttgaataaac gtacagccca
     1621 taaccatttg tcaattctta cttcttcttt tatcatatca tcctacttat tataagtaac
     1681 tttgctaaaa gaaactattt gttgttgtac agattcatgg tatgttgaat acctgccaat
     1741 acaaaacttt gtattatgtc aattgctatt tgcatgcgag aggggagagc agtattttct
     1801 tctaacgtcc atttgccaag cacatagttg atttgttgac ctttggggta gttgttgcca
     1861 attccaaatc gtaaacgagg gtattgatcg gtacctaaaa tttcttgaat attctttaat
     1921 ccgttatgac cagcatcgct tcctttcgat tttagtcgca ataccccaaa aggtaaagct
     1981 aaatcatcta ctaagataag tacattttct atcggaattt tttcttcttt catccaataa
     2041 cgaactgcat ttccacttaa attcataaaa gtagagggtt taagtaaaat cattgttttg
     2101 tttttgattt tacattcaca cacagccccg taccgttttt cggtaaaaaa aacattggat
     2161 gccttagcga aggcatccaa tagtgtaaat cctatgttgt ggcgagtatc ttggtattcg
     2221 ctaccaatgt tacccaatcc tacaatcaag tatttcatat gttattattt ttcgcctttt
     2281 gcagctaatc ctcttgcagc tcttgttaat tttacagcta ctattacgtt gtctttgttg
     2341 ttcaaaattt cgaggtttgg atagctcaac tgtccagctt ttaatgtttt tcctaaacct
     2401 aatttttcaa catttacaac caaattttct ggaatgtctt tgcaaagagc ttttactttt
     2461 agcttgcgta attccaatga taatttaccc cctgctttta caccttctgc caagccttca
     2521 acttttacag gcacagataa tacaattggt tttttgttgt caacttctaa aaagtcgatg
     2581 tgtaaaactt tgtctgtaac agggtgaaat tgtaagtctt tcattacagc tacacttttt
     2641 ttcccatcta cagttaagtg tacttcaaaa atttcgggtg tgtaaattaa tttacgaaca
     2701 tcgctttcgg ttactgtaaa gtgagtgttg ctaccagttc cgtacattac acaaggtatt
     2761 gcgttgtcgt tacgtactgc tttacttggt tttttaccta agtctgttct tgttactcca
     2821 gataattcaa atgttttcat gttaattttt tgttgtgtta ctcaaattca agtccagtta
     2881 taatattaag tgattagtga cttgattccc atcacacttt ctttcgaaaa gcggtgcaaa
     2941 ggtatgtatt ttttttttat ttacaaaaga gatacttagt catttttatg taatgtattc
     3001 caaccttgag cttttaattg aaattcttgt ccgtctcgtg taattaaatt acagcctaac
     3061 gattcttttg tagtatgtcc aataatgcta attccttcta aagttgctat tttttcgtgc
     3121 atggataatg gaacagtaaa caataattcg taatcttctc cgccatttaa agcagctgtt
     3181 accaaattca tattgaattc attagccatt gatgccgttt ggtaatcaat tggaattttg
     3241 tcttcgtata tgcgacatcc aactttactt tgggtacata tatgcaatgt ttcagacgat
     3301 aagccatcag atatgtcaat cattgaagtc ggaataatgg attcttttgc aagtaattcg
     3361 atgatatctt tacgggcttc gggttttaat tgtcgttgta aaatatattc ttttccagta
     3421 aaatcaggct gtgcattatt cgtttgacgg aaaagtgttt tttcacgttc taataattgc
     3481 aatcccatgt aggctgcacc caaattgcct gacacacaaa ttaaatcacc ctcttttgct
     3541 gtattacgat acgcaatagt ttttgtatta actacaccaa tgcaggtaat actaatagtg
     3601 agtccagtaa gagaggctga agtgtcccct ccaataatgt ctaccttata cgtagagcaa
     3661 gccagtttaa tcccctcata taattcttca atatcttcaa tacagaaacg gttagataca
     3721 ccaatagaaa cagtaatttg ttgaggagtg ccattcatgg catatatatc cgaaaagtta
     3781 acaatagccg ccttgtatcc caaatgtttt aaaggaaaat aggttaaatc gaaatgaata
     3841 ccttctaata ataaatcggt tgaaataagt gtttgagaat taccctcatt attttctatt
     3901 actgcggcat catctcctat acctttaata gaggttggat gttgtagtga aatggtactt
     3961 gctaatcgct caattaagcc aaattctcct aattctgaaa tagttgttcg tgtttgcata
     4021 catttttttc ttgaattgca aagatataga tattttttta catcccaatg tcaaagagct
     4081 acagctgtta tctattttga aatgataaaa aaaataaatt tgtagttaaa tatgatataa
     4141 actccctttt tatgtttaat tttgctcaaa atttacagaa ccttattctg catgagtctg
     4201 tatattgata gtttatctaa gttttatggt acccagcatg tattgaaaaa tatttcattt
     4261 catgtagaaa gtggtgaaat tattggtttt ctaggtccga atggagcagg gaagtcaact
     4321 gcgatgaaga ttattactgg ctttgtacag ccagatagtg gtagtatatt tgttgatgga
     4381 attgatgtgt tgaatgatac tttacatgca caaaaaataa ttggatattt gccagagcat
     4441 aatccattgt atttggatat gtatgttgcc gaatacttat tgtttattgc tcagctttat
     4501 aaattaggta aagaaagtaa acagagagta gaggatataa tagaaaagac agggttaact
     4561 cgagaatata caaaaaaaat tggacaatta tcaaaagggt atcgtcaacg agtgggttta
     4621 gctcaagcca tgattcataa tcccaaagtg cttattttgg atgagcctac tactggtctt
     4681 gatcctaatc aattagttga aatacgtgaa cttattaaag aggtaggcaa agataaaaca
     4741 gtaatactta gcacgcatat tatgcaagaa gtgcaggctg tgtgtgatag atatgttatt
     4801 atacgagaag gatcaattgt tgccgatagc aaaaaagata agattcatga tactatgacc
     4861 atcgttgtgg aatttgcaaa tccaatatcc aaagaacatt tattcatgat agataagata
     4921 actgctatac acgcaattaa tgcacggtcg tatgtggttg aatgtaatga agatatacgg
     4981 tatttgttgt atcaatttgc cgtagaacaa ggggtgggct tggtaacctt gcacttacag
     5041 gagcaaaatt tggaagaact gtttagaaaa ttaacgcgaa attagttgat atagagtaaa
     5101 ataaacatag atggaagata atttcgatat aagaaaagga gctttaaccg ataaagaaca
     5161 agatgttgat attgccttac gtccattaag ttttaatgat tttagcggac aaaaaagcat
     5221 tgtggacaac ctaaaaattt ttgttcaagc agctcgtatg cgtggagaat cgttagacca
     5281 cgtattattg catggacctc ctggattag
//
LOCUS       DAOPUR010000032         5270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_15679_length_5270_cov_3.068648, whole genome shotgun sequence.
ACCESSION   DAOPUR010000032 DAOPUR010000000
VERSION     DAOPUR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15679_length_5270_cov_3.068648"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1303
                     /locus_tag="PKN90_00975"
     CDS             224..1303
                     /locus_tag="PKN90_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNZ84890.1"
                     /translation="MNYNNYCVIMAGGVGSRFWPFSKNSMPKQFLDFFGTGRTLLQMT
                     FDRFAKVVPASNILIVSNKDYKDIILEQLPLINPSQVLLEPNRRNTAPCVAYAVSRIK
                     AINPKANMVVAPADHLILKEIDFLNVIERSLNFVNEHPNLLTLGIKPNRPETGYGYIQ
                     ISDQAMGDFKKVKTFTEKPNLELAKVFVESGEFFWNSGVFVWSVEAIWKAFNEFLPEM
                     VAKFEAGSDKFNTDLEQDFINEIFPSCQNISIDYGIMENAKNVYVLCGDFGWSDLGTW
                     GSLYDMSEKDEHQNVTLKCKTQFYESNDNIVVLPENKLAVIQNLSGYIVVESDNVLLI
                     CKKEDEQRIRHFVTDVHLKYGEGYI"
     gene            1307..2059
                     /gene="lptB"
                     /locus_tag="PKN90_00980"
     CDS             1307..2059
                     /gene="lptB"
                     /locus_tag="PKN90_00980"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNZ84891.1"
                     /translation="MLSENQMVLRTEHLVKRYKTRTVVDNVSISVKQGEIVGLLGPNG
                     AGKTTTFYMTVGLVTPNQGRIFLNDLDISSYPVYKRAQQGIGYLAQENSVFRKMSVED
                     NILSVLQMTKFSKKYQHEKLETLISEFSLQTVRKSLGDQLSGGERRRCEIARCLAIEP
                     KFIMLDEPFAGVDPIAVEDIQRIVAQLREKNIGILITDHNVHETLSITDRAYLLFEGK
                     ILFQGTAEELADNPVVREKYLGASFELRKKVE"
     gene            complement(2113..2700)
                     /gene="folE"
                     /locus_tag="PKN90_00985"
     CDS             complement(2113..2700)
                     /gene="folE"
                     /locus_tag="PKN90_00985"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNZ84892.1"
                     /translation="MEKEKPASSPEILQELASHYKAVIELIGENPTREGLLKTPERMA
                     KSMQFLMRGYNQDPEAIIRSAMFTEDYRQMVIVKDIDFYSMCEHHMLPFFGQAHVAYI
                     PNKKITGLSKIARVVDVFARRMQVQERLTTQIKECIQNTLNPLGVMVVIEAQHMCMQM
                     RGVEKQHSVTTTSDFTGIFEQAKTREEFISLISKK"
     gene            complement(2704..3165)
                     /locus_tag="PKN90_00990"
     CDS             complement(2704..3165)
                     /locus_tag="PKN90_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNZ84893.1"
                     /translation="MAKQVLFSILFGFLMAIQGMAQENPLENDSLLQEETILSEHDRM
                     NTILYRAKNTATSEIEFVSIGGFRVQVYAGNHPQKAREDAMLIDKTIKEQYPEYHAYV
                     SFQAPFWRVRIGDFASYYEALLLSRELKKIFPDFGNEIYVVKDPTVRILYF"
     gene            3280..3966
                     /locus_tag="PKN90_00995"
     CDS             3280..3966
                     /locus_tag="PKN90_00995"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNZ84894.1"
                     /translation="MSNIQGKYVVVVAGGKGKRMQSEMPKQFLLLRGKPILMYTLETF
                     FLYDHSIQIILVLPEDDIDMWKKLCQQHAFELAHTIVNGGNERFYSVANGLTLVPEGV
                     LVAIHDGVRPFPSTSTIERTFGMAAEKGNAIPVVELTDSIRQLSDNQENKSVSRAAFR
                     LVQTPQTFQSTQIKKAYTQPYSPLFTDDASVLESVGESIFLVEGNRENIKITTPLDLV
                     IGEYLLDQRK"
     gene            4049..>5270
                     /locus_tag="PKN90_01000"
     CDS             4049..>5270
                     /locus_tag="PKN90_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ84895.1"
                     /translation="MDADIMYLPGMGPKRAQLFNKELSIFTVKDLIFHFPYKYIDRSR
                     FYYIHEIDTTLTYIQVRGKITGFQLLGEGRNQRLIGKFTDGRNVIDLVFFKGIKYCTQ
                     AYQLHTEYIVFGKPSLFNGTFSFVHPDIEPYKEEVKMGFQPLYHTTEKMKSKFVNSKA
                     VQKLIYQALQTITPSLPESLPTSILHKYRFISLYEALINIHFPSNMPLLEQAQARLKF
                     EELFYIQIAILRQAHALKTSFKGFQFNHIGEMFHAYYEKCLPFELTNAQKRVLKEIRN
                     NVRGAHQMNRLLQGDVGSGKTIVALMCMLMAIDNGYQACIMAPTEILARQHFETIESQ
                     INKLPIQVKLLTGSTKKKDREHIHEGLLNGSVHILIGTHAVIEDTVNFKNLGIVVIDE
                     QHRFGVAQRAKLWNK"
BASE COUNT         1727 a          884 c          984 g         1675 t
ORIGIN      
        1 tctgggcaat tggagagtgc aaaagtacat aaaattttta tttgagcaaa acagaaactt
       61 cttttttctg aaaatatttt tttattacct tgctttccgt atctttgcct ccaaattaag
      121 tagatgggtt ttataaagtg agatatctaa aataaataac cctgatgttt tctatttttt
      181 tacaaaaatg taatttcaca acaccaatta aactatcaat cgtatgaatt acaacaacta
      241 ctgtgttatt atggcaggtg gggtaggaag tcgcttttgg cccttcagta aaaattcaat
      301 gccaaaacaa tttttggatt tttttggtac aggtcgtacc ttgcttcaga tgacttttga
      361 ccgttttgca aaagtagtac cagcatcaaa tatactcatt gtaagcaaca aagattacaa
      421 agatatcatt ctagaacaat tacctctcat caatccatct caagtgttgt tagaaccaaa
      481 ccgcagaaat acagcccctt gtgtagcgta tgcagttagt cgcatcaaag caattaatcc
      541 aaaagccaac atggtagtgg ctccagctga ccacttgatt ctaaaagaga tagatttttt
      601 gaacgtaata gagcgtagtt taaattttgt aaatgagcat cctaatttgc ttactctggg
      661 cattaagcca aatcgtcctg aaacgggcta tgggtatatc caaataagcg atcaggctat
      721 gggcgatttt aaaaaagtga aaacgtttac cgaaaaacca aaccttgaat tagcaaaagt
      781 ttttgtcgaa agtggcgaat tcttttggaa ctcaggagta tttgtatgga gtgtggaagc
      841 aatttggaaa gcattcaacg agttcttacc cgaaatggta gccaagttcg aagcaggtag
      901 cgataaattt aatacagact tagaacaaga ttttatcaac gaaattttcc catcgtgtca
      961 aaacatttct attgactatg gtattatgga aaatgccaag aatgtgtatg tattgtgtgg
     1021 tgattttggg tggtctgatc ttggtacatg gggttcattg tacgatatgt cagaaaaaga
     1081 tgagcatcaa aatgtaacac ttaaatgcaa aactcaattt tacgaaagta acgacaatat
     1141 tgtggtattg cccgaaaata aattagcggt tattcaaaac ctttctggtt atattgtggt
     1201 agagtctgac aatgtgttgt tgatttgtaa aaaagaagac gaacaacgca tacgtcattt
     1261 tgtaacggat gtacatctta aatacggcga gggatatatt taatctatgt tatcagaaaa
     1321 tcaaatggta ctccgcacgg agcatttagt aaaaaggtac aaaacacgta cagttgttga
     1381 caatgtttcc atctcagtga aacagggtga aattgttggc ttgcttggtc cgaatggagc
     1441 cggtaaaaca actacgtttt atatgacagt aggcttggta acacctaatc aaggacgtat
     1501 ttttctaaac gatctcgata tatcttcgta tccggtttat aagcgagctc agcaaggaat
     1561 tggatatttg gctcaagaaa attcggtgtt tagaaagatg agtgtggaag acaatatttt
     1621 gtcggtatta caaatgacaa aattctctaa aaaataccaa cacgagaagt tggagacatt
     1681 aatcagtgag tttagcttgc aaacagtacg caaaagtttg ggagaccaac tgtctggcgg
     1741 tgagcgtcgt aggtgcgaaa tagcacgttg tttagccatt gaacctaagt ttattatgct
     1801 tgatgagcca tttgcaggtg tcgatcctat tgctgtggag gatattcagc gtatagtagc
     1861 acagttgaga gaaaaaaaca ttggcatttt aattaccgac cataatgtgc acgaaacctt
     1921 gagtattaca gatagagcat atttgttgtt tgaaggaaaa attctttttc agggaacagc
     1981 cgaggagcta gcagataatc ctgtagtgcg agaaaaatat ttgggtgcca gttttgaatt
     2041 acgaaaaaaa gtggaataaa aaataaagca gacttttggt ctgctttatt ttttttacga
     2101 tgctcaaatc aattattttt tgcttatcaa actaataaat tcttctctag ttttagcttg
     2161 ttcaaaaatt cccgtaaaat cagaagtagt tgtaaccgaa tgttgctttt ctactccacg
     2221 catttgcata cacatatgtt gagcctcaat tacaaccatt acgccaagcg gatttaaagt
     2281 gttttgaata cactctttga tttgtgtagt taatcgttct tgtacttgca tacgacgagc
     2341 aaatacatct actacgcggg cgattttact caatcctgta atttttttgt tggggatata
     2401 agctacatgt gcttgtccaa aaaacggtaa catgtgatgt tcgcacatcg aataaaaatc
     2461 aatgtctttt acaattacca tttggcggta atcttcggta aacattgccg aacgaataat
     2521 tgcttctggg tcttggttgt aaccacgcat caaaaactgc atactttttg ccatgcgttc
     2581 gggcgttttt agcaaacctt ctcttgtagg gttttctcca ataagttcaa ttacagcttt
     2641 ataatgggat gccaattctt gcaatatttc tggagaacta gctggttttt ctttttccat
     2701 acgttaaaaa tataaaatac gaacagtggg gtctttaact acataaattt cgttcccaaa
     2761 atcagggaat atctttttta attctctgct taataataac gcttcgtagt acgatgcaaa
     2821 gtctcctata cgtactcgcc aaaagggggc ttgaaaagat acataagcat ggtattcagg
     2881 atattgttcc ttgatagttt tgtctataag catagcatct tctcgtgctt tttgaggatg
     2941 gttgcccgca tacacttgta cgcgaaatcc accaatactt acaaattcaa tctcagaagt
     3001 tgctgtgttt ttggctctat acaaaatggt attcatacga tcatgctccg atagtattgt
     3061 ttcttcttgc aataaactgt cattttccaa aggattttct tgagccattc cttgaattgc
     3121 cattaaaaaa ccaaaaagaa tactgaataa tacctgtttt gccatacttt tttcgttatt
     3181 ttatgtgcaa aaataccaaa atttattcta aaaatggctt ttacaggtat ttttgtagaa
     3241 aaatagtact tttgtacaaa ttataacacc caatttttta tgtcgaacat acaaggaaaa
     3301 tatgtcgttg tagtagctgg cggtaaagga aaacgtatgc aaagtgagat gccaaaacaa
     3361 tttttattgt tgcgtggtaa accaattttg atgtatacct tagaaacatt ttttttatac
     3421 gatcattcca tacagattat tcttgtattg cccgaagacg atatagatat gtggaaaaaa
     3481 ttatgccaac aacatgcatt tgaactcgca catacaattg ttaatggagg gaatgagcgt
     3541 ttttattccg ttgccaatgg gttaacgcta gtgcccgaag gggtattggt agctatacat
     3601 gatggcgtac gaccttttcc tagtacatct actattgaac gtacatttgg tatggcggct
     3661 gaaaagggga atgctattcc tgttgttgaa ctaactgatt ctattcgtca actatccgac
     3721 aatcaggaga ataaatccgt tagtcgtgct gcatttcgtc tagtgcaaac tccgcaaaca
     3781 tttcaatcta cacagattaa aaaggcttat acgcaaccat attcaccctt atttacggat
     3841 gatgcaagtg tcttggaatc agtaggagag tcaatttttt tggtagaagg aaatagagaa
     3901 aacataaaaa taactactcc gctagatttg gttattggag agtatttgct ggatcaacga
     3961 aaatagataa agtgaaaggc ttcgattttt aaattctttt gctatatttg ctcgtacata
     4021 cactaaaccc aattaagtga gtttactttt ggatgcagac ataatgtatt tgcctggcat
     4081 gggaccgaaa agggctcagc tattcaataa agaattatca atctttactg taaaagattt
     4141 gatatttcac tttccgtata aatacatcga tagaagccga ttttattata ttcacgaaat
     4201 agacacaacc cttacatata ttcaggtaag aggcaaaata actggatttc agttgttggg
     4261 agaaggacga aaccaacggt taataggaaa gtttactgac gggcgaaatg taattgattt
     4321 ggtcttcttt aaaggaataa agtattgcac acaagcatac caattacata ctgaatatat
     4381 tgtatttggt aaacctagct tatttaatgg aacattctct ttcgttcatc cagatattga
     4441 accatacaaa gaagaagtta aaatgggttt tcagcccttg taccatacca ccgaaaaaat
     4501 gaaaagtaag tttgtgaatt ctaaagctgt tcaaaaactc atttaccaag ccttacaaac
     4561 tattacacca tcattacccg aatctttacc aactagtatc cttcacaaat accgttttat
     4621 ttcactttac gaagcattaa tcaatattca tttccccagc aatatgcctc ttttagagca
     4681 agcacaagca cgactaaaat ttgaagaact attttatata caaattgcaa tattacgtca
     4741 ggctcatgct cttaaaacta gttttaaagg gtttcaattt aatcacattg gagaaatgtt
     4801 tcatgcctat tacgaaaaat gcttgccctt tgaactaacc aatgctcaaa aaagagtact
     4861 taaagagata cgtaataatg tacgtggagc tcatcaaatg aataggttgt tgcaaggcga
     4921 tgtaggtagt ggcaaaacta ttgtagctct aatgtgtatg cttatggcta ttgataatgg
     4981 ttatcaggca tgtattatgg ctcctactga gattttagcc aggcaacatt ttgaaactat
     5041 tgaaagccaa ataaacaaat tacccataca agtaaaatta cttacaggtt caacgaaaaa
     5101 aaaagacaga gaacatattc acgagggctt actgaatggt tctgtacata ttttaattgg
     5161 cacacatgct gtaatagaag atacagttaa ttttaagaat ttaggcatag tggtaattga
     5221 tgaacaacat cgttttggtg tggcacaacg agctaaattg tggaataaaa
//
LOCUS       DAOPUR010000033         5255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_15749_length_5255_cov_2.820192, whole genome shotgun sequence.
ACCESSION   DAOPUR010000033 DAOPUR010000000
VERSION     DAOPUR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15749_length_5255_cov_2.820192"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKN90_01005"
     CDS             complement(<1..355)
                     /locus_tag="PKN90_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNZ84896.1"
                     /translation="MKIFNSTAIRQIDAYTIEKNHIKSIELMEVAARSVFEYIIQHYN
                     IEKKIIIFAGQGNNGGDALALARLLHIAGFSIKIITVFLSDNLSDDCETNYKVLLNKN
                     IPIYHYSRYLELNIKE"
     gene            complement(411..1043)
                     /locus_tag="PKN90_01010"
     CDS             complement(411..1043)
                     /locus_tag="PKN90_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ84897.1"
                     /translation="MNVKTFIFNPFQENTYVLSDSNNKCIIIDAGCLNLEEKNDLYSY
                     IKENNLLVERILNTHLHIDHCFGNQFVSETFNKKIEAHPDDEALLNGMNKYAQTFGIE
                     YTENHPTISYIFEGDTITVGEISLKAIHVPGHSKGSLVFYAEKEKYLFAGDVLFSGSI
                     GRTDLPGGNFHDLIDNIKQKLLILPDETVVFSGHGAQTTILHEKQYNPFL"
     gene            complement(1071..1697)
                     /locus_tag="PKN90_01015"
     CDS             complement(1071..1697)
                     /locus_tag="PKN90_01015"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNZ84898.1"
                     /translation="MHQLKYAARRKELVEILAMKGNFSTHVLQAIGKVPRHLFMDKTL
                     IEFAYEDKAFPIGSNQTISQPYTVAYQSELLNIQKGDKVLEIGTGSGYQSAVLVELGA
                     QVFSIERQKVLFHHTYSLLPEIGYRIRMFHGDGYVGLPKYAPFDKIIVTASAEEIPQE
                     LIQQLKIGGIMVIPVGNTMFKVTKLTDTTTEKIEVDNCAFVPMLRGIN"
     gene            complement(1706..2326)
                     /gene="rsmG"
                     /locus_tag="PKN90_01020"
     CDS             complement(1706..2326)
                     /gene="rsmG"
                     /locus_tag="PKN90_01020"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNZ84899.1"
                     /translation="MNVIIKYFPNLSIQQVAQFEALFPLYMDWNSKINVISRKDIENL
                     YVHHVLHSLAIAKIIPFVDQTSVIDIGTGGGFPGIPLAILFPNVQFHLVDSIGKKIKV
                     AQEIAQEIGLENTTFLHDRIENEKKTVDFVVSRAVMPLGDLVRFCKKNIKKEQKNSLP
                     NGLICLKGGELQSEIQAFKKSATIYELTDFFNEDYFKTKKAVYIPL"
     gene            2440..2898
                     /locus_tag="PKN90_01025"
     CDS             2440..2898
                     /locus_tag="PKN90_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84900.1"
                     /translation="MKRFILQFFAVLLFFASLGVNVVHYGCDACREYFPILMGQSCMD
                     VHHSVKGDLAICCTSSCDDSEAQDIPHKHDDHHQDCTSQRLSIHLDSYIAKVQITQPL
                     STIPSFLLSAVLAIPSDIQQINGKKEYLSINLYKYYLYEDYLSKIAVFII"
     gene            3050..5218
                     /locus_tag="PKN90_01030"
     CDS             3050..5218
                     /locus_tag="PKN90_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ84901.1"
                     /translation="MKTFYIFLIGLLVSASMHSQIIGKVLGENKEPLIGANIYWENTN
                     YGVLSSIDGNFEIEKMNTSDRLIISYLGYKSDTIQITYSSPYIVQLAQVTELDEVEVS
                     GRKSGLVTFRATPIQTQEISYSELCRAACCNLSESFETNPSVDVSYTDAATGAKQIRL
                     LGLSGIYVQMINENIPSFRGLGSTYGLSYVPGTWMDGILISKGTSSVSNGYESITGQI
                     NVEYKKPQLADPFSVNLFGSSDGRVELNADASTKLTKNLSTMLFVHADKDFQHHDKNN
                     DGFADYPQVEQYSFFNRWQYANKGYTAQFGAKGLYENRRGGEMIHHGYKINIDTWRAD
                     AFMKHGFELNKEKESSIGVIANGVFHRQESIYGIQTYDASQTSLYLNAIYQSHLTDEE
                     THLIKTGLSMEADLYEEKWNTQITETDYITPGIFGEYTLKLADKWIVLAGIRADYNKQ
                     YALFVTPRLHIKYMPINAVTLRFSIGKGYRSPNVMIENNNLLASNREFVISTPLQQEA
                     AWNTGVNAVFDIPIGYRTVMVSTEYYYTLFDNQLVIDFDSFANKILFYNALGTSYAHN
                     AQIEVSTEIADGLTILGAYRYNDTKSVTGGVLQLKPLMSMHRGLITASYKTSLGRWQY
                     DITSQLNGGGRMPTPDAINPLWNKTFSAYQQYNAQVTRYFKNASVYVGIENITNFTQS
                     NPIISASNPQSDTFDATMIWGPLHGRKFYLGLRYNLPKQN"
BASE COUNT         1724 a          806 c          887 g         1838 t
ORIGIN      
        1 cttcttttat gtttagttct aagtatctgc tataatggta gataggtata tttttattaa
       61 gtagtacttt atagtttgtt tcacaatcat ctgataaatt atccgataaa aacacagtaa
      121 ttattttgat tgaaaatcct gctatgtgta acaaacgagc tagggctaaa gcatctccac
      181 cattgttgcc ttgtcctgca aaaatgatta tttttttttc tatattataa tgttgtataa
      241 tatattcaaa tacagaacga gcagctacct ccattaattc aattgattta atgtggtttt
      301 tttcaatggt ataagcgtct atttggcgaa tagctgtaga attgaatatt ttcataccat
      361 tggaaattag atttggaggt acatattatg aggagatgat atgttacgtt ttataaaaaa
      421 gggttgtatt gtttttcgtg caatatggtt gtttgagccc catgaccaga aaaaacaact
      481 gtttcatcgg gcaaaataag taatttttgc ttaatattat caattaaatc gtgaaagttt
      541 cctccaggaa gatctgttct tccgatggaa ccactaaata atacatcacc tgcaaaaaga
      601 tatttttctt tttctgcata aaagactaag ctcccttttg aatgccctgg cacatgtatt
      661 gcttttaatg aaatttcacc tactgtaatg gtatctcctt caaaaatata actgatagta
      721 gggtgatttt cagtatattc aattccgaac gtctgagcat atttattcat accatttaat
      781 agagcttcat catcgggatg agcttcaatt tttttattaa atgtttctga aacaaattga
      841 ttaccaaagc aatggtcaat atgtagatga gtatttaaaa tacgttcaac gagtaaatta
      901 ttttctttaa tgtaggaata caaatcattt ttttcttcca aatttaggca acctgcatca
      961 ataataatgc acttattgtt gctgtcgctc aatacatagg tattttcttg aaaagggtta
     1021 aaaataaatg tcttaacgtt cattttattg ataatattca tgttaatata ttaattaatt
     1081 ccacgtagca taggcacaaa agcacaattg tctacttcaa ttttttctgt tgttgtatcg
     1141 gttaatttgg taactttaaa cattgtattt cctacaggaa tgaccataat accacctatt
     1201 ttaagttgtt gaatcaattc ttgaggtatt tcttctgcac tagcagtaac tataattttg
     1261 tcaaacggtg catatttggg taatcccaca tagccatctc catgaaacat ccgaatacga
     1321 tacccaattt caggtaatag cgaatatgtg tgatgaaata ggactttttg acgttcaatg
     1381 ctaaaaactt gagctcctaa ttcgactaaa acagctgatt gatatccaga tcctgtgcct
     1441 atttctaaca ctttatctcc tttttggatg ttcagtaatt ccgattggta ggcaacagtg
     1501 taaggttgag aaatagtttg gtttgatcct ataggaaatg ctttgtcttc gtatgcaaac
     1561 tcaattagcg ttttatccat aaataaatgt cgaggaactt tgccaattgc ttgcaataca
     1621 tgtgttgaga aatttccttt cattgctagt atttctacta actcttttct tcttgcggcg
     1681 tattttagtt gatgcataat aacttttaca aaggaatata aacagctttt tttgttttaa
     1741 aataatcttc attgaagaaa tcagttaact catatattgt tgcacttttt ttaaaagctt
     1801 gtatttctga ttgaagttcc cctcctttta aacatattaa tccattaggg agggaatttt
     1861 tttgttcttt tttaatattt tttttacaaa aacgaactaa atctcccaga ggcataacag
     1921 ctctactaac cacaaaatca actgtttttt tttcattttc gattctatcg tggagaaagg
     1981 ttgtattttc aagtccaatt tcttgagcta tttcttgagc tactttaatt tttttaccga
     2041 ttgaatcaac taaatgaaac tgtacgttag gaaacaatat agctaacgga ataccaggaa
     2101 aaccgccacc agtgccaatg tcaataacag atgtttgatc aacaaaaggt ataatctttg
     2161 ctatcgctaa tgaatgtaat acgtgatgaa cgtataaatt ctcaatatct ttacgtgaaa
     2221 tgacgttaat tttgctattc caatccatat ataatggaaa aagtgcttca aattgagcaa
     2281 cttgttgtat tgataaattt gggaagtatt taataatcac attcatgctt aaaaaattaa
     2341 aaattcataa ttttatgtca aagatacgtt ttttctatat ctgtaaaaat tttttgtgtg
     2401 tatctttgca aaaaagtaat taacactact ttcttgtcaa tgaaacgatt tattttacag
     2461 ttttttgccg tattactctt ttttgctagt ttaggagtaa atgttgttca ctatggatgt
     2521 gatgcttgta gggagtattt tcctatttta atgggtcagt catgcatgga tgtacaccat
     2581 tcagtaaagg gtgatcttgc tatctgttgt actagttctt gtgatgatag cgaagcacaa
     2641 gatatccccc acaaacatga tgatcatcat caagattgta catctcaacg cttatctata
     2701 catttggata gttacatagc taaggtgcaa ataacacagc ctctatctac tattccgtct
     2761 tttttacttt ctgctgttct tgcaatacct tctgatattc aacaaattaa tggtaaaaaa
     2821 gaataccttt caattaattt gtataagtac tatttgtacg aagattattt atccaaaata
     2881 gctgttttta ttatttgatt tgaactaaac tctagtttta tctatgtatc atcattgtgt
     2941 ccatttacga tataatgggt tgtactcaag ataaaagtaa gaaaaaaggg tgtgtttgtt
     3001 acacatttac gttttttatt ttagtttatt cacttcattt tttttaataa tgaaaacatt
     3061 ttatatcttt ttaattggat tgctggtatc tgcatccatg cattctcaaa taattggcaa
     3121 agtgttggga gaaaacaaag aaccccttat tggtgctaat atttattggg aaaatacaaa
     3181 ttatggtgtt ctttcttcta ttgatggaaa ttttgagata gaaaaaatga atacatcgga
     3241 tcgtttgatt ataagttatt taggatataa atcagacaca atacagatta catattcttc
     3301 tccctatatt gtgcaattgg cacaagttac agaattagat gaagtagagg tttcgggcag
     3361 aaaatcaggt ctagtaactt ttagggctac tcctattcaa acgcaagaga tatcttattc
     3421 agaattatgt cgtgctgctt gttgtaattt gtctgagagt tttgaaacaa atccttcggt
     3481 agacgtctct tacacagatg cagctacagg tgcaaagcaa attaggttgt taggtttatc
     3541 aggtatttat gtacaaatga taaatgaaaa tataccaagt tttagaggat taggtagtac
     3601 ctatggttta tcgtatgtgc ctggtacgtg gatggatggt attttaattt ctaagggaac
     3661 atcctctgta tctaatggat atgaatcgat tacagggcag attaatgttg agtacaaaaa
     3721 acctcagtta gcagacccat tttcagtgaa tctgtttggt agtagcgatg gtagggttga
     3781 acttaacgct gatgcaagca ctaagctgac aaagaatttg tcaacgatgc tatttgtgca
     3841 cgcagataaa gattttcaac atcatgataa aaataatgat ggattcgctg attatccaca
     3901 agtagagcaa tattcatttt ttaacagatg gcaatatgca aacaagggct acacagcaca
     3961 gtttggagct aaaggcttat acgaaaatag aagaggcgga gagatgatac atcatggcta
     4021 taaaatcaat atagatactt ggagagctga tgcttttatg aaacatggct ttgaattgaa
     4081 taaagaaaaa gagtcgagta ttggtgtgat agcaaatggt gtatttcatc gccaagaaag
     4141 catatatgga attcaaacat acgatgcatc acaaacgtca ttgtatctaa atgcaattta
     4201 tcagagtcat ttaactgatg aagaaacgca tttaattaaa acagggctaa gtatggaagc
     4261 ggatttatat gaagagaagt ggaataccca gataacagaa acggactaca ttactcctgg
     4321 tatttttggt gagtatacat taaaactggc agataaatgg attgtgttgg caggtatacg
     4381 agctgattat aataaacaat atgctctatt tgtaacccca cgtttgcata ttaaatacat
     4441 gccaataaat gctgttacct tgcgtttttc tataggtaaa gggtatcgat cgccaaatgt
     4501 aatgatagag aataataact tacttgcaag taacagagag tttgttattt ctactccact
     4561 acaacaagaa gctgcttgga atacaggcgt aaatgcagta tttgacattc caattggcta
     4621 ccgaacagta atggtttcta cagagtatta ttatacatta tttgacaatc aacttgttat
     4681 tgattttgat tcatttgcta ataaaatcct tttttacaat gcattaggca cgtcttatgc
     4741 acacaatgca caaatagaag ttagcactga aatagcggat gggttaacaa ttcttggagc
     4801 ttatcgatat aatgatacga aatctgtaac aggaggtgta ttacaactaa aaccactaat
     4861 gagcatgcat agaggtttaa taacagcatc ttacaaaact tccttaggca gatggcagta
     4921 tgatattacc tcgcaattaa atggtggcgg acgtatgcca acgcctgatg caataaatcc
     4981 attatggaat aaaacgttta gtgcctacca acaatacaat gctcaagtta ctcgttactt
     5041 taaaaatgca tctgtgtatg taggtattga aaatattact aactttacac aatcaaatcc
     5101 aattattagt gcttctaatc cacaatcaga tacatttgat gctacaatga tttggggacc
     5161 tttgcacgga agaaaatttt atctgggttt acgctacaat ttaccaaaac aaaattaaat
     5221 atataaatat gaaaaacaaa aatgtattta gcata
//
LOCUS       DAOPUR010000034         5230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_15854_length_5230_cov_3.522512, whole genome shotgun sequence.
ACCESSION   DAOPUR010000034 DAOPUR010000000
VERSION     DAOPUR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15854_length_5230_cov_3.522512"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKN90_01035"
     CDS             <1..410
                     /locus_tag="PKN90_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HNZ84902.1"
                     /translation="KSKTGAIIVIQNDADLSAYAKTGEKIEAYIKARLIENIFFKNTP
                     LHDGAMIIANNRIKAVGCILPISHTQDIPKNLGLRHRAALGISQKTDATTIVVSEETG
                     KISLSERGKLYFNISPEELLFFLTNGVDVENKK"
     gene            456..1961
                     /locus_tag="PKN90_01040"
     CDS             456..1961
                     /locus_tag="PKN90_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HNZ84903.1"
                     /translation="MQTSFQVVSAAEAASYINDGDVIGFSGFTPAGSTKEVAVAIADR
                     AKSFHAEGKPFKIGMYTGASTGDSLDGVLARANAVSFRTPYQSNKDLRTSINDNNVNY
                     FDMHLSMIAQELRYGFMPRPNVAIIEACDITNDGEIVLTSAVGITPTIVRLADKIIIE
                     LNHYHPKGLKGIHDIYEPLDPPYRKEIPIYKPSDRIGSPTLKIDPKKILCIVETNKPD
                     EVGGFSPIDDITKRIGENVANFLANEIKRGTIPPQFLPIQSGVGNIANAVMASLGENP
                     GIPPFEMYTEVIQDSVISLMKQGSIKFASGCSLTVTTPVLQDIYANLDFFKERLVLRP
                     QEISNSPEVSRRMGLIAINTALEADIFGNINSTHVMGTKMMNGIGGAGDFTRSAYLSI
                     YTTPSTAKGGKISAIVPMVSHVDQSEHSVKIIITEHGVADLRGKSPIQRAHEIIDNCV
                     APEYKDILNDYLKIHAKDQTPHCLCSCFGMHSEFLKSGNMLNIDWKEYENK"
     gene            1971..2507
                     /locus_tag="PKN90_01045"
     CDS             1971..2507
                     /locus_tag="PKN90_01045"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNZ84904.1"
                     /translation="MIPFTKQAIRQLTKLKVLALSSFERIEKEITIFAQLEQLQEFKN
                     AKHILCYWALPDEVNTSMLIRTWEKEKKFYLPVVKNTELVIKPYSSSKELQQGVFAIQ
                     EPRTDSEVSLSDIDLIIVPGVAFDELGHRLGRGKGYYDRLLQHTTTTKIALAYSEQIY
                     ESIPTELHDIKMDIVVSA"
     gene            2520..4163
                     /locus_tag="PKN90_01050"
                     /pseudo
     CDS             2520..4163
                     /locus_tag="PKN90_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(4228..>5230)
                     /locus_tag="PKN90_01055"
     CDS             complement(4228..>5230)
                     /locus_tag="PKN90_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNZ84905.1"
                     /translation="KKKNSAFEVCEAIYYLAYKNNKIVGRIAGIVNHRANEAWGVKKT
                     RFGWFDFIDDTDVSFSLLKAVENWGKAKGMDSINGPVGFTDFDHEGLLIEGFDQLGTL
                     ATIYNYPYYAKHYEAYGLTKDIDSLEWKIYTPKEVPVRMARISALVKEKYNLRYVKVK
                     SHKELVKRFGYTFFDLIDEAYRPLYNYSPLTESQKKYYCEIYFPMLNFDFLSLVVNDK
                     DELVALGVSMPDITKALQKCRGKLFPFGFIHILKGLKAKKIDVIDLMLIAVRPDYQNK
                     GVNSLLFDEQTPYYNKYQVKYSESSPVLETNLKSQANYAYFETEQHKRRRIYIKPIV"
BASE COUNT         1769 a          963 c          979 g         1519 t
ORIGIN      
        1 ccaagtcgaa aacaggggct atcattgtca ttcaaaatga tgctgaccta tctgcatatg
       61 caaaaacagg ggaaaagatt gaagcataca ttaaagcacg attaatagaa aatatttttt
      121 ttaaaaacac ccccttacat gacggtgcta tgataatagc aaataaccga atcaaagcag
      181 ttgggtgcat ccttcctatt tcacatacac aagatatccc aaagaaccta ggtctgagac
      241 atagagctgc cctcggtatc tcgcaaaaaa cagatgctac caccatagta gtttctgaag
      301 aaacgggtaa aatatcgctc tcagaaagag gaaaattata ctttaatatt tcccccgaag
      361 aattactgtt ttttttaacc aacggagttg acgttgaaaa taaaaaatag tatctttgtt
      421 gccttaaaaa caacatacta aaatacttaa ttaacatgca aactagtttt caagtagtat
      481 ctgcagcaga ggctgcaagc tatatcaatg atggggatgt aatcggattt agtggattta
      541 cccctgccgg ttcaacaaaa gaagtagcag tagctattgc tgacagagca aaaagttttc
      601 acgcagaagg taagcctttc aaaataggca tgtatactgg agcttctacg ggcgattcgt
      661 tagatggcgt attggctcgt gctaacgcgg tttccttccg tactccttac caatcgaaca
      721 aagatttacg tacgagtatt aacgacaata acgtaaatta ttttgatatg catttgtcca
      781 tgattgctca agaactacgc tacggattta tgcctcgccc caatgttgcc attattgaag
      841 cttgtgatat aactaacgat ggcgaaattg tgcttacatc agctgtgggt attaccccaa
      901 ccattgttcg tttagccgac aaaattatta tcgaattaaa tcattatcat ccaaaaggat
      961 taaaaggaat tcacgatatt tatgaacctc tagacccccc atatagaaaa gaaattccta
     1021 tttacaaacc aagcgatcgt attggttcgc ccacgttaaa aattgatcca aaaaagattt
     1081 tatgtatcgt tgaaacaaac aagcctgacg aggtaggtgg tttctctccg attgacgata
     1141 taacaaaacg tattggtgaa aatgttgcca actttttggc gaatgagatt aaaagaggaa
     1201 ccattccacc acaattttta cccatacaat ctggcgtagg taatattgcg aacgcagtaa
     1261 tggcttcatt gggtgaaaac ccaggaattc ctcctttcga aatgtacaca gaagttattc
     1321 aagattctgt tatctcgtta atgaaacaag gaagtataaa atttgctagc ggttgctcgc
     1381 ttactgtaac aacacccgta ttgcaagata tttatgccaa tctagatttc ttcaaagaaa
     1441 gattagtatt acgtccacaa gaaatatcaa acagtccaga agtatctcgt cgcatgggac
     1501 taattgccat taatactgca ttagaagctg atatttttgg aaatatcaac agtactcacg
     1561 taatgggtac aaaaatgatg aatggtattg gtggtgctgg tgattttaca cgtagtgctt
     1621 atttatcaat atatactaca ccttctactg caaaaggtgg taaaattagt gctattgtgc
     1681 cgatggtgtc gcatgttgac caaagcgaac attctgtaaa aataattatc actgaacacg
     1741 gggtagctga tttacgtgga aaaagcccta tccaacgagc ccatgaaata attgataatt
     1801 gtgtggcacc agaatacaaa gatattttaa atgattattt gaaaattcat gctaaagacc
     1861 aaactcctca ctgcttatgc tcttgttttg gtatgcactc agaatttttg aaatcaggca
     1921 atatgttgaa tattgattgg aaagaatacg aaaataaata actaaatgtt atgataccgt
     1981 ttacaaagca agccatcaga caacttacaa aactgaaagt gcttgctttg tcttcttttg
     2041 agcgaattga aaaagaaatt actatatttg cacagttaga gcaattacaa gaatttaaaa
     2101 atgctaaaca tatactgtgc tattgggctt taccagacga ggtaaacaca tctatgctaa
     2161 ttcgcacttg ggaaaaagaa aaaaaatttt atttgcctgt ggtaaaaaat actgaattag
     2221 tgataaaacc atatagctca agcaaagaat tgcaacaagg agtttttgct attcaagaac
     2281 caaggacaga tagtgaggta agtttgtcgg atatcgactt aattatcgtt ccgggtgtag
     2341 catttgatga attagggcat cgattaggaa gaggcaaggg gtattacgat agattgttac
     2401 aacatacaac aacaacgaaa atagcgttgg catatagcga gcaaatttac gagagcatac
     2461 ccacagaatt acatgatata aaaatggaca ttgttgtgtc cgcataaacg atatatagta
     2521 tggcaatacc aacatttaag taccaagaac catttccgct aggtaaagat actacggaat
     2581 attatttact tactgaccag tatgtttcag tcagcaattt tgaaggaaaa gaaattttaa
     2641 aagttgatgt agaaggctta acgcaagttg ccaacgtggc tatgcgtgat gtggcattta
     2701 tgctcagacc tgctcaccaa caacaagtag cagcaatttt agccgacccc gaagccagcg
     2761 aaaacgacaa gtatgtggct cttactatgt tgcgtaatgc tgaaatatcg gcaaaaggag
     2821 ttttaccttt ttgtcaagat acaggtactg ctacagtatt tgcaaaaaaa ggtcaacatg
     2881 taatgaccaa tggcaatgat gaagaagctt tatctttcgg tatttacaaa acatataccg
     2941 aagaaaattt gcgttattcg caaaccattc cgttggatat gtacaacgaa ataaattcag
     3001 gaacaaatct acctgctcaa attgacatca tgagcacaca gggtgatgaa tataaatttt
     3061 tatttgtagc caaaggaggc ggttctgcaa ataaaaccta tttatttcaa gaaaccaaag
     3121 ccttgctaaa ccctgcaaca ttggaaaaat tcctagttga aaaaatgaaa accttaggca
     3181 cttctgcctg tccaccctat catatagcat ttgtaatagg tggcacttcg gctgatgcag
     3241 cagtaaaaac ggtaaaactt gcctctacca aatattacga cgaactgccc acaaaaggca
     3301 acgaaggcgg acaagcattt agagatatag aattagaaca aaaaatactt gctgcggctc
     3361 aaaaatcggg ctttggtgct caatttggcg gcaaatactt tgctcacgat gtgcgtatca
     3421 ttcgtttgcc tcgccacgga gcatcttgcc ctgtagcgat ggctgtatcg tgttcggctg
     3481 atcgcaatat aaaggcgaaa attaacaaaa aaggcatctg gatagaaaaa atggaagaca
     3541 atccagggaa atatattcct gaatcaatgc gtaaagcggg cgaaggtgat gcgatacaca
     3601 ttgaccttaa ccgtccgatg aaagaaatat tggctgaatt atcaaaatac cctgtggcta
     3661 ccagattatc actcaatggc accattattg taggtagaga tattgctcat gcaaaattaa
     3721 aagaacgcat tgatagcgga gaaggtttac cacaatacat taaagatcat cctatttact
     3781 atgcaggacc agcaaaaacg cctcaaggca tggcgtgtgg ttcgatgggt cccactacag
     3841 ctggacgtat ggattcgtaa gtggatttgt ttcagtcgca aggaggaagc atgattatgt
     3901 tggcaaaagg aaaccgcagt caagcagtaa ctgacgcttg tcaaaaatac ggcggttttt
     3961 atcttggttc tatcggcgga ccagcggcta ttttagctca aaacaacatc aaaaaaattg
     4021 aatgcatcga atatcctgaa cttggaatgg aagctatttg gaaaattgaa gtagaagatt
     4081 ttcccgcttt tatcttggtt gatgacaaag gaaatgattt tttcaaacaa ctgaaacctg
     4141 gttgtgtaaa ggctctgaaa taatagcatt tcattagaca aaaaaatggg agaaatctac
     4201 aaacgatttt ctcccatttt ttgtttatta aactatcggc ttaatataaa tacgacgacg
     4261 tttgtgttgt tcggtttcaa aataggcgta attggcttga ctctttaagt ttgtttccaa
     4321 cacaggcgat gactccgaat attttacttg atatttattg taataaggag tttgttcatc
     4381 aaataacaac gaatttaccc ctttgttttg ataatctgga cgtactgcaa ttaacatcaa
     4441 atcaattaca tcaatttttt tagcttttaa cccctttaat atatggataa aaccaaatgg
     4501 gaataattta cccctgcatt tttgtaaggc tttggttata tcaggcatac tcactcctag
     4561 tgcaaccaat tcgtctttat cattcactac caaggataga aaatcaaaat taagcatagg
     4621 gaaataaatt tcgcaatagt attttttttg actctccgtg agtggagaat aattgtataa
     4681 gggacgatat gcctcatcta ttaaatcaaa aaatgtgtat ccaaaacgtt ttaccaattc
     4741 tttgtggctt tttaccttga catagcgtaa attgtatttt tcttttacca aggctgaaat
     4801 acgtgccata cgtacgggaa cttctttggg ggtataaatt ttccattcaa ggctatcaat
     4861 gtctttggtt aagccatacg cttcgtaatg ctttgcataa tacggataat tataaatagt
     4921 ggcaagtgtt cctaattggt caaatccctc aataagtaaa ccttcgtggt caaagtcagt
     4981 aaatccaaca ggtccattga tgctatccat ccctttggct tttccccaat tttcaacagc
     5041 ttttagtaaa ctaaacgata catctgtatc atcgataaaa tcgaaccaac caaaacgcgt
     5101 ttttttaaca ccccaagcct cattggctct atgattaact ataccagcta tgcgaccaac
     5161 aattttattg tttttatagg ctaaataata aattgcctca cacacttcga aagcagaatt
     5221 cttcttttta
//
LOCUS       DAOPUR010000035         5229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_15863_length_5229_cov_2.679552, whole genome shotgun sequence.
ACCESSION   DAOPUR010000035 DAOPUR010000000
VERSION     DAOPUR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15863_length_5229_cov_2.679552"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PKN90_01060"
     CDS             <1..868
                     /locus_tag="PKN90_01060"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HNZ84906.1"
                     /translation="LSVFIDAMILHFIIKRYSRYYVLAFIIYIVFYGYIFEMDQIRNA
                     KAVMLFLLSLRYVLNNKFLSYCLLNLIGFTFHSSSILFLFIYPFLKKRFSLTFYAVVF
                     IIGIVLFVLQIEYIKPMVMYVASKMGGVFQTKAEIYIASPIYGKDMFGITLGFFERIV
                     SYCLFCFIYYKKLIDRDKENLLFINLYVLFFIFYFYFAEFIVLAVRTSALFIAAYFVL
                     YPNVLFEINRKLTRQLVVIMLTIFMFFRMAAYVKYIEYKYENILFDTSSYKIRKSYAD
                     ESANYLQELDKK"
     gene            865..1320
                     /locus_tag="PKN90_01065"
     CDS             865..1320
                     /locus_tag="PKN90_01065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ84907.1"
                     /translation="MISIIIVNYNTALFVNKAIESILLHTKDISSEIIIVDNNSSQDL
                     QFHISVFKEKVSVIYLAENIGFGRANNEGLKIATGRYVFFLNPDTILLNNAVGAQANN
                     RSLVHKVEHFMQIWRANKQQFVQARLVKQLSVFDYINYKIRYNRYIQNK"
     gene            1325..2218
                     /locus_tag="PKN90_01070"
     CDS             1325..2218
                     /locus_tag="PKN90_01070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ84908.1"
                     /translation="MDVSVIFVNYNSIHLLQNAIASVIEHTHGVQYEIIVVDNNSSDN
                     AKNLLQEYFPSVPIQFVALSANIGFGRANNEGFKRAQGRHIFFLNPDTILLNDAIAIL
                     SGYLENEPKVGVCGGNLYTEKMLPMHSFSRVLPSIIWQVNLLFFGLPFKAIYGKNTDF
                     NHTEIPIPVGYVTGADMMIKRSVLDKTGGFSDDFFMYFEETELSFRIKNQGYKIMSIP
                     QAKIIHLKGQTFSCNEIKQRYYVESWHTYFRKRYNTVGYTCAKSIFLFHATLKYIFAV
                     CTRKKERMRIYKQFFIWGKKA"
     gene            2215..3258
                     /locus_tag="PKN90_01075"
     CDS             2215..3258
                     /locus_tag="PKN90_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNZ84909.1"
                     /translation="MIYINGRFLTQKPTGVHRFSFEMCKALHTLGLDFVVLSPPHILD
                     FYTCNFPIKIIGKTSSHLWEQIELPLFLKSKKSPLLISFMGLGCVLYSNQISTIHDVS
                     FLRNPQWFSKAYYYFYKIMTPWMARKSKKIITVSNFSKHELIELLQINENKLCVIYNA
                     VAKHFFEKSEILPINEPYILAVSSLDPRKNFSNLIHAFLKLQDLPHKLYIIGGNSSVF
                     STMNLPISDKIVFLNYVSNHDLVAYYKQASLFVYPSLYEGFGIPPLEAMSQQCPVLVS
                     DIQVLHEVCKDAVFYCNPNDSNDIANQIHYILTNPSEVEIKINIGLQHINNFSWKASA
                     SKLLEVIKELDRE"
     gene            3286..4407
                     /locus_tag="PKN90_01080"
     CDS             3286..4407
                     /locus_tag="PKN90_01080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ84910.1"
                     /translation="MKKILHISKYYPPYKGGIEDVCYNIVRILHKSNSCQQKVICFSG
                     EKETTNELYDGVHVLRVGSTMQIARQVITLSYRKELKKMIQEFKPDIIHFHAPNPLIA
                     YYLLSIIPPHTELIVHWHSDIVAQRQLYKLVKKVEDRLLQRASVILATSPNYIDDSLP
                     LKKYTYKVEILQNFIDPSKFLLTPDLLNKVDAIKKQYNNVPILFFLGRHVYYKGIQFL
                     IDAEPLITHDCVLLIGGKGELTEELKRKNKSSRIHFLGRIDDEDLPMYYCASDIFVFP
                     SVTKNEAFGVALAEAMYCKNAAVTYTIPGSGVNWVSLHGVTGIESENGNIEQLSRAIN
                     TLLADTELRKTYAEAAKKRVEDNFTIEIIEERLKKLYSI"
     gene            4690..>5229
                     /locus_tag="PKN90_01085"
     CDS             4690..>5229
                     /locus_tag="PKN90_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNZ84911.1"
                     /translation="MKTVAIQGVAGSFHDIAVRNFFKEQEIKLVPCDSFKELFAVIKK
                     DNSIIGAVAIENTIAGSLLPNHTLLRESGFKIVGEYKLRIEHQLVAMPGQTIKDIEKV
                     YSHPIALMQCEDFLVEHPHLEPVESEDTAASAKLIAEKKIMGRAAICSRLAAEIYGLE
                     VLAEGIETNKRNYTRFLIIA"
BASE COUNT         1770 a          758 c          918 g         1783 t
ORIGIN      
        1 tctttctgtt tttattgatg cgatgatttt acatttcatt attaaaagat attcaagata
       61 ttatgtttta gcatttatta tttatatcgt tttttacggg tatatttttg agatggacca
      121 aattagaaat gcaaaagctg tgatgttatt tttgctttca cttaggtatg ttttaaataa
      181 taaattttta tcctattgct tattaaatct tattggattt acatttcatt caagctctat
      241 cttattcctt tttatctatc cgttcttaaa aaaacgtttt tcactcacct tttatgcagt
      301 tgtgtttatt attggaattg ttttatttgt tttgcaaata gaatatatta agcctatggt
      361 aatgtatgta gcgtccaaaa tgggtggagt ttttcaaaca aaagcagaaa tatatatagc
      421 gtcacccata tatgggaaag atatgtttgg aattacactt ggcttttttg agcgaatagt
      481 gtcttattgt ttattctgtt ttatttacta taaaaaattg attgatagag ataaagaaaa
      541 tcttttattc attaatttgt atgttttatt ttttattttc tatttctatt ttgccgaatt
      601 tatcgttctt gcagtacgaa catctgctct ttttatagca gcttactttg tattgtatcc
      661 gaatgtatta tttgaaataa acagaaagct aactagacaa cttgtagtta ttatgttaac
      721 tatatttatg ttttttcgta tggctgctta cgtgaaatat atagaatata aatatgaaaa
      781 tattcttttt gacacatcat catacaaaat aagaaaatcg tatgccgatg agtctgctaa
      841 ttatttacaa gaattagata aaaaatgata tccataatca tcgtaaatta taataccgct
      901 ttatttgtaa acaaagcaat tgaatccata ttgctacata caaaggatat atcatccgaa
      961 attattattg tagataataa ttcttctcaa gatttacaat ttcatatttc tgtttttaaa
     1021 gaaaaagttt cagtaattta tcttgctgaa aatattggct ttggaagggc aaataacgaa
     1081 gggttaaaaa ttgcgactgg tcgttatgta ttttttctaa atccggatac gatattactc
     1141 aataatgcag taggagcaca agcgaataat cgttcattag tgcacaaggt cgaacatttt
     1201 atgcaaatat ggagagctaa caagcaacaa tttgtacaag caaggttagt taaacagttg
     1261 tcagttttcg attatatcaa ctataaaata cgctataata ggtatattca aaataagtaa
     1321 tgctatggat gtttcggtaa tttttgttaa ctataattct attcacttgc tgcaaaacgc
     1381 tattgcaagt gtaatagagc atactcatgg tgtgcaatat gaaattatag tggttgacaa
     1441 taattcttcg gacaacgcta aaaatttatt gcaagaatac ttcccatccg ttcctatcca
     1501 gtttgttgca ttgtccgcaa atatcggatt tggcagagct aacaacgaag gttttaagcg
     1561 tgctcaagga aggcatattt tctttctcaa cccagataca atattgctaa acgatgcaat
     1621 cgctattctg tcaggctatt tagagaacga acccaaggta ggtgtatgtg gtggtaattt
     1681 atatacagaa aaaatgctac ctatgcactc gttttcgcgt gtattacctt ccataatttg
     1741 gcaagttaat ttgttgtttt ttggtttgcc atttaaagct atttatggca aaaataccga
     1801 ttttaatcat acagaaatac ctattccggt aggatatgtt acaggagcag atatgatgat
     1861 taaacgttct gtgttagaca aaacaggcgg tttttcggac gattttttta tgtattttga
     1921 ggaaacagaa ttgtcgttca gaattaaaaa tcaaggctac aaaattatga gtatcccaca
     1981 agcaaaaatc atccatttaa aaggtcaaac gttttcgtgt aatgaaatta aacaacgtta
     2041 ttatgttgaa tcttggcata cttattttag aaagcgctac aatacagtag gatatacttg
     2101 tgccaaaagc attttcttgt ttcatgctac attaaagtat atctttgcag tttgtacacg
     2161 aaaaaaagaa agaatgagga tttataaaca attttttatt tggggcaaaa aggcatgatt
     2221 tatatcaacg gaagattttt aacgcaaaaa cctactggtg tacatcgttt ttcgttcgaa
     2281 atgtgcaaag ccttgcatac tttggggctt gattttgttg tgttgtcacc ccctcatata
     2341 ttggattttt atacctgtaa ttttccaata aaaataattg gcaaaacttc atcgcatttg
     2401 tgggagcaaa ttgagttacc tctatttctg aagagcaaga aatctccttt attaatcagc
     2461 tttatgggct tgggttgtgt gttatactcc aatcaaataa gtacgataca cgatgtttcg
     2521 tttttacgaa atccgcaatg gttttcaaaa gcatattatt atttttacaa gataatgaca
     2581 ccttggatgg caaggaagtc aaagaaaatt ataacggtaa gtaatttttc aaagcacgaa
     2641 ttgatagagt tacttcaaat caatgaaaac aaactgtgtg taatttacaa tgcagtagcg
     2701 aaacattttt tcgaaaaatc agaaatctta ccaatcaacg aaccatatat tttggcagtt
     2761 tcttcgttag atccacgcaa aaatttttca aatttgattc atgcgttcct aaaattacag
     2821 gatcttccac acaaattata tataattggt ggaaattcga gcgttttttc cacaatgaat
     2881 ttgccaattt cagataaaat tgtatttttg aactatgtat ccaatcatga tttggttgcg
     2941 tattacaaac aagcatctct ttttgtgtat ccatccttgt acgaaggctt tggaataccg
     3001 ccattagagg ccatgagtca gcaatgccca gtgctagttt ctgatattca agtattgcat
     3061 gaggtgtgta aggatgccgt tttttattgc aatccaaatg atagtaacga tatagccaat
     3121 caaatacact atatattgac gaatcctagc gaggttgaaa ttaagataaa tattggtttg
     3181 caacatataa acaattttag ctggaaagca tcagcaagca agttgttaga ggtaatcaag
     3241 gagttagata gggaataaac gtaatgtgat aagggtaagt tataaatgaa aaaaatactc
     3301 catatatcca agtattatcc accatacaaa gggggcattg aagatgtgtg ctataatata
     3361 gtgcgcattt tgcacaaaag caacagttgc caacaaaagg taatctgttt tagtggcgaa
     3421 aaagaaacaa ctaacgaact atatgatgga gtacatgtgc ttcgtgttgg ttctactatg
     3481 cagattgcac gtcaagtcat tacattatcg taccgcaaag aactgaaaaa aatgattcag
     3541 gaattcaagc ctgatattat tcattttcat gcaccaaatc ctttaatagc ctattatttg
     3601 ttgtctatta taccacctca tacagagttg atagtacatt ggcattctga tattgtagcc
     3661 cagcgtcaat tatataaact agttaaaaag gtagaagata gattgttgca acgagcatct
     3721 gttattcttg ctaccagccc taattatatc gacgattcat tgccattgaa aaaatataca
     3781 tataaagtag aaatattaca gaactttatt gatccttcta aattcctact gacacccgac
     3841 ttattaaaca aggtggatgc aattaaaaaa cagtacaata acgttcctat tttattcttt
     3901 cttggcagac atgtatatta taaaggtatt caatttttga tagatgctga acctcttatt
     3961 acacatgatt gtgtgctttt gattggagga aaaggggagt tgaccgaaga gttaaaacgc
     4021 aaaaataaaa gttctcgtat tcatttccta gggcgtatag atgatgagga tttaccgatg
     4081 tattattgtg catcagatat atttgtgttt ccttctgtaa ctaaaaacga agcttttggg
     4141 gtggcattag ccgaagctat gtactgcaaa aatgctgctg ttacctatac aattccgggt
     4201 tcgggagtta attgggtctc gttgcatggt gtaactggaa ttgagtctga gaatggtaac
     4261 atagaacaat tatcacgtgc cattaataca ttattggcgg ataccgagtt gagaaagacc
     4321 tatgcagagg cagcaaaaaa acgagtagaa gataatttta caatcgaaat aatagaagaa
     4381 agattaaaaa aactgtatag tatttaattt gctgttggat attttatgtg aaaacataaa
     4441 gcttgacaaa aaaactttca attttctatt gtttgttaaa aaaaaactgt atttttgtgc
     4501 cagaataata ttgaaatgag aaattttact tttacatact tttactttta tttttacttt
     4561 agcaaaaagt aaaacggggt ttggtatgaa aaatgtaaac aataacataa taagtaagta
     4621 aatcccgtca aaattgatgg gattttttaa ttttaagcta tatagataac gaaatataaa
     4681 actaaaaaca tgaaaacagt tgctatacaa ggtgtcgcag gttcctttca cgatattgca
     4741 gtccgcaatt tttttaaaga acaagaaata aagttagtgc catgcgattc gtttaaagaa
     4801 ttatttgcgg ttattaaaaa agataattca attataggag cagtggcaat tgaaaatacc
     4861 attgcaggaa gtttgttgcc taatcatacc ttgttgcgcg aaagtggctt taagattgtt
     4921 ggtgaatata aattacgtat agaacatcaa ttggtggcga tgccaggcca aacaataaaa
     4981 gatattgaaa aagtatattc gcatcctata gcattaatgc agtgtgagga ttttttagta
     5041 gaacaccctc atttagagcc tgttgagagc gaagatacgg cggcaagtgc taaattaata
     5101 gcagaaaaaa agattatggg cagagcagcg atttgtagcc gtttagccgc tgaaatatat
     5161 ggattggaag tgttggctga aggcattgaa acaaacaaac gcaattatac tcggttttta
     5221 attatagcc
//
LOCUS       DAOPUR010000036         5198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_15994_length_5198_cov_3.583900, whole genome shotgun sequence.
ACCESSION   DAOPUR010000036 DAOPUR010000000
VERSION     DAOPUR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15994_length_5198_cov_3.583900"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKN90_01090"
     CDS             <1..237
                     /locus_tag="PKN90_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84912.1"
                     /translation="FYSKPTPKPVVTETIDMDEWDDDATMQQFESVPAFKRNNIDTPN
                     TQPSASERSSIFIEKTDDDVLFSQKNKFLHDNVD"
     gene            240..689
                     /locus_tag="PKN90_01095"
     CDS             240..689
                     /locus_tag="PKN90_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNZ84913.1"
                     /translation="MELIDQITEDIKKAMLAKEKTVLDALRGIKKELLEAKTAKGGDG
                     TVSEEMGTKILQKMVKQRKESAQIYTEQNRKELADKELEEAEIIARYLPKQLSDEELT
                     TAIQTIISQVGATTAKDMGKVMGIATKQLSGKVEGKLISEKVKSLLQ"
     gene            732..1946
                     /locus_tag="PKN90_01100"
     CDS             732..1946
                     /locus_tag="PKN90_01100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ84914.1"
                     /translation="MNKKRKYLLLVISLLLITIGGMVWQRWNVWFGNRPEMPFTSKPY
                     PHRILLTLGENTNQRILSWQHDSIIQQATVKLTKLHSTDTLVITAKGNIVRSRGGVTA
                     FYAAQLNDLSSNAQYAYQVLSGEKQTEWYQFKTTSTNSPFSFMYIGDVQDRDTSNTSK
                     QAFQYIYQQFPEADFWAFGGDQIERPMNSYWEAFMNDLDGIRQSTPIIAIAGNHEYLK
                     YAIRKIDDRFTYTFPYFINNHNNNHVFTFVYGNVRFILLDSNREPWYIWQQRNYLKEV
                     LKNNSSLFTVVMLHHPVLSNKSSWNNASIRLMMEPILSQYPVDLVLQGHEHGYARNSK
                     KDKSGTSKLPIYVTSHLSKKFYPSFKATKITDAIIPNTALFQHIKVDNNILTLYSYTL
                     QGEKVDSVSIVK"
     gene            1956..3647
                     /locus_tag="PKN90_01105"
     CDS             1956..3647
                     /locus_tag="PKN90_01105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ84915.1"
                     /translation="MNKNKLSANQLFWILNIAWMLLNLLQASVTELANDEAYYVVFAK
                     HLDWGYYDHPPMIALFIYLGQWLSGELGVRLLTVLSQPIYIWLLWKLIAPATVEKKDV
                     WLFFIIALAMPILHIYGFVAVPDAPLMLFSALFLYFFVQYLQHTSWYYIPFMALAIAA
                     IGYSKYTGVLVVLFACAAYPKVFLRKGIYITATLSLLLMLPHLYWQYQHEWVSFQYHL
                     VGRNQDFNAFYIAEYVGNFIAVFNPFILPLVVWIGFKNRHQDELTLLILKRIALFFFL
                     FFFASCIRGHVQPQWFIFVTFSVIALVWQWARNHEKRYRYIRTVSIISIVILIGFRIV
                     AATNPFGMKAEVFNNKTSYTQIANATGNLPVIFMGKYTMAAKYSYYTGLDAHASPNIH
                     FRTSQYQLWDFDSNLLEKPVAIHVGGDYPNATHIQTANGQFSFVIDSCYMPIRKVQIQ
                     WLNPYNTIASKREEKNMTLSIHNPYPYDIVIDNEKIQLHYIIRKPWSWVEYFPVDVEN
                     PLILTAFSTTNIQQKISIESEKAKPNETYETGFLLTKPPYIVWYNSPIYNIQIVP"
     gene            3748..4119
                     /locus_tag="PKN90_01110"
     CDS             3748..4119
                     /locus_tag="PKN90_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNZ84916.1"
                     /translation="MIIQFIKFCVVGASGLLVDFGITYLCKEKIKLNKYIANSLGFIA
                     AASSNYLLNRMWTFENTDPNISTQYLLFIIISLIGLGINNGVIYLVLRKRDYNFYIVK
                     VFAIAVVTLWNFFMNFFFTFQ"
     gene            complement(4203..4976)
                     /locus_tag="PKN90_01115"
     CDS             complement(4203..4976)
                     /locus_tag="PKN90_01115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ84917.1"
                     /translation="MSNENKSIHEFDYQLICDYFSRIERQGPGSPEVTVKALSFIDKL
                     NETSKIADLGCSTGGQTMVLAQHTQGSIVGLDLFPEFIALFNKNAQLQHLQDRVKGIQ
                     GSMDKLPFKTEELDLIWSEGAIYNIGFERGLREWKTFLKKGAYIAVSEATWFTDERPD
                     EITAFWQDAYPEIDTIPAKIAKMQACGYIPVASFILPENCWVEHYYKPQIEAQKVFLQ
                     QHKGNKIAEELVANERHEAQLYAKYKEFYGYVFYIGKKL"
BASE COUNT         1712 a         1008 c          876 g         1602 t
ORIGIN      
        1 ttttactcta aaccaacccc caaacctgtt gtaacagaaa ctattgatat ggacgaatgg
       61 gatgacgatg ctacaatgca acagtttgaa tcagttccag catttaagcg taataatatc
      121 gataccccaa atactcaacc atcggctagt gaacgttcat caatttttat agaaaaaacg
      181 gatgatgacg tattattctc tcaaaaaaat aaatttttac acgataatgt tgactaatta
      241 tggaactaat agaccaaata accgaagata ttaaaaaagc catgttggct aaagaaaaaa
      301 ccgttcttga cgctctacgt ggcattaaaa aagaattgtt agaagccaaa acagccaaag
      361 gtggcgacgg cactgtgagc gaagaaatgg gtacaaaaat tcttcaaaaa atggtaaaac
      421 aacgcaaaga atctgctcaa atctataccg aacaaaaccg caaggagtta gccgataaag
      481 aacttgagga agcagaaatt attgctcgct atttacccaa acaattatct gacgaagaac
      541 ttactactgc tattcaaacc attatctcgc aggtaggtgc caccacagct aaagatatgg
      601 gcaaagtaat gggcatagcc accaaacaat taagcggaaa agtagaagga aaacttattt
      661 ctgaaaaagt aaaaagtttg ttgcaatagc ttaccatgta aactattgtt ttatacacta
      721 tacccttgcc aatgaataaa aaaagaaaat acctcctact tgtaataagt ctgttgctta
      781 taacaattgg aggtatggtt tggcaacgat ggaatgtgtg gtttggcaat cgccccgaaa
      841 tgccttttac aagtaagccc tacccacaca gaatcttact taccttgggg gaaaacacca
      901 accaacggat acttagctgg caacacgact ctattataca acaagcaacg gtaaaactaa
      961 ccaaattaca ttctactgac acgcttgtaa ttactgccaa aggaaacatt gtacgctcgc
     1021 gtggaggagt aacagccttt tatgctgcac aattgaacga tttatcaagt aatgcacaat
     1081 acgcatatca agtactttcg ggcgaaaaac aaactgaatg gtaccagttt aaaacaacat
     1141 caactaatag tccgttctct tttatgtaca taggcgatgt gcaagacaga gataccagta
     1201 ataccagcaa acaggctttt caatacatat atcaacaatt tcctgaggct gatttttggg
     1261 cttttggtgg cgaccaaatt gagcgtccaa tgaatagcta ttgggaggct tttatgaacg
     1321 accttgatgg cataaggcaa tctactccaa ttatagctat tgctggcaat cacgaatacc
     1381 ttaaatatgc catccgcaaa attgatgata ggtttactta tacattccct tattttatca
     1441 ataaccataa caacaaccat gtatttactt ttgtatatgg taatgtgcgg tttatcttgc
     1501 tcgactcaaa tcgtgaacct tggtacattt ggcaacaacg aaattaccta aaagaagttt
     1561 taaaaaacaa ctcatctcta tttacagtgg taatgctaca ccaccctgta ttatcaaaca
     1621 aaagtagctg gaacaatgca tctatccgtt tgatgatgga acctatttta tctcaatacc
     1681 ctgttgattt ggtactacaa ggacacgaac atggatatgc tcgtaattca aaaaaagaca
     1741 aaagtggcac tagtaaacta cctatttacg taacctctca tctgtcaaag aagttttatc
     1801 ccagctttaa agcaacaaaa attaccgatg caattattcc aaacacggcc ttatttcagc
     1861 atataaaagt tgacaataac atcttaactt tatattcata caccttacaa ggagaaaaag
     1921 tagatagtgt gagtattgta aaataacccc ttactatgaa taagaataaa ctgtcagcca
     1981 atcaactatt ttggattttg aacatagcat ggatgttgtt aaatctgttg caagccagtg
     2041 taacagaact agccaatgat gaagcatatt acgtagtatt tgccaagcat ctcgattggg
     2101 gttattatga tcatccaccc atgattgcct tgtttattta tcttgggcaa tggttatctg
     2161 gcgagttagg tgtacgtctg cttacggttc tatcacaacc aatctatata tggttactat
     2221 ggaaactgat agcacctgcc acggtagaaa aaaaagatgt ttggttattt ttcatcatcg
     2281 cattggctat gcccatattg catatttatg ggtttgtagc ggttcccgat gcacctctga
     2341 tgcttttttc agcactattt ttatacttct ttgtgcagta tctgcaacat acatcttggt
     2401 actatattcc attcatggca ttggcaatag ccgccattgg ttatagtaaa tatacaggtg
     2461 tgctagtagt actatttgct tgtgcagcgt atcctaaagt atttttacgc aagggaattt
     2521 acattacagc cactttatcc ttattgttaa tgttacctca cttatattgg caatatcaac
     2581 acgaatgggt atcgtttcag tatcatcttg taggcagaaa tcaagatttt aatgcctttt
     2641 acatagcaga atacgtaggt aattttattg ctgtattcaa tccatttatt ctgcctttgg
     2701 tagtatggat tggtttcaaa aaccgacatc aagacgagct tacactgcta atcctaaaac
     2761 gcatagcttt atttttcttt ttattctttt ttgcctcatg catacgcgga cacgtgcaac
     2821 cgcaatggtt tatttttgta accttcagtg ttattgcatt ggtttggcaa tgggctagaa
     2881 accatgaaaa aaggtatcgc tatatacgta cagttagtat tatttccatt gttatactga
     2941 taggtttccg cattgttgca gccactaatc cgttcgggat gaaagctgag gtgtttaata
     3001 ataaaacaag ctacactcaa attgccaatg ctacaggaaa tttaccagta atatttatgg
     3061 gtaaatacac tatggcagct aaatacagtt attatacagg tttagatgca catgcttcac
     3121 ccaatatcca ttttagaacc agccaatacc aactatggga ttttgatagc aacttactag
     3181 aaaaaccagt agcaattcac gtaggtggcg attaccctaa tgctacacat atacaaactg
     3241 ctaatggtca atttagtttt gttatagata gttgctatat gcctatccgc aaggtacaaa
     3301 tccagtggct taacccctac aatacgatag catcaaaaag ggaagaaaag aatatgactc
     3361 tatcaataca caatccgtat ccttatgata tagtgataga taacgaaaaa atacaacttc
     3421 attacattat ccgaaaacct tggtcgtggg ttgaatactt tcctgttgat gtagagaatc
     3481 cgcttatttt aacagcattt tcaaccacca acatccaaca aaaaattagt attgaaagcg
     3541 aaaaagccaa gccaaatgaa acatacgaaa caggcttttt gcttaccaaa ccaccatata
     3601 ttgtgtggta caacagcccc atatataaca tacagatagt accctgaaaa actgtatata
     3661 gtaagctaac aagaaaaaag cacaaaaaag cacctacaat gaattccttt tatttatctt
     3721 tgccattata aatagtataa cagaacaatg attattcaat ttatcaaatt ttgtgtagta
     3781 ggagcatctg gcttattggt tgattttggc attacctatc tgtgtaaaga aaaaattaag
     3841 ctcaataaat acattgccaa ttcattaggc tttattgctg cagctagtag caactaccta
     3901 ctcaatcgta tgtggacatt tgagaacact gaccccaaca tctctacaca atatctgctg
     3961 tttatcatta tttctcttat tggtttgggc attaacaatg gagtgatata tttggtgctt
     4021 cgcaaacgag actacaattt ttacatagtt aaagtttttg ccatcgcagt ggtaacactt
     4081 tggaacttct ttatgaactt cttttttact tttcagtgaa aagatgcaac gaatttgatt
     4141 cactcactat ccgttaaaac aagcggattg cccaccaaac aggcaatccg cttttaaaat
     4201 agctacaatt tctttccaat ataaaagaca tagccataaa actccttgta cttggcgtac
     4261 aattgagctt cgtgtcgttc gttagccaca agctcttcgg ctattttatt ccctttgtgt
     4321 tgttgcaaga atactttttg agcctcaatt tggggtttat aataatgctc tacccagcag
     4381 ttttcaggta atataaaact agctactgga atatatccac aggcttgcat tttagctatt
     4441 ttggcaggta ttgtgtctat ctcgggataa gcatcttgcc aaaacgcagt aatttcgtct
     4501 ggacgttcat cggtaaacca cgttgcttcg cttacagcaa tgtaagctcc ttttttcaga
     4561 aatgtcttcc attcccttaa tccacgttca aatccgatgt tataaattgc tccttctgac
     4621 caaatcaggt caagttcctc agtcttaaaa ggtagcttat ccattgagcc ctgtatccct
     4681 tttaccctat cttgaagatg ttgtagttgt gcgtttttat taaataacgc tataaactca
     4741 gggaacaaat cgagccctac aatgctacct tgggtatgtt gtgctaacac catagtttgc
     4801 ccacctgtac tacaacctaa atctgcaatt tttgaggttt catttagctt gtcgataaaa
     4861 ctcaaggctt ttacggttac ttcaggacta cctggtcctt gacgttcgat acgtgaaaaa
     4921 tagtcgcaaa ttaattgata atcaaattcg tgaattgatt tattttcgtt actcatgata
     4981 attaaaattg tttcgttaat aaatatggct aaaatacata cgtattcaat gccaactaat
     5041 aaatagttat gataaactac tcggacaaga atatgcctga gtaccagttg tagggataag
     5101 tatagaaaat tgcctacact tatttactta accaaatccg aatttaacgt aaacatccaa
     5161 ttattttaga ttgtgcaaat atacaaggtt ttaggtat
//
LOCUS       DAOPUR010000037         5111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_16377_length_5111_cov_2.786392, whole genome shotgun sequence.
ACCESSION   DAOPUR010000037 DAOPUR010000000
VERSION     DAOPUR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16377_length_5111_cov_2.786392"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..195
                     /locus_tag="PKN90_01120"
                     /pseudo
     CDS             1..195
                     /locus_tag="PKN90_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            394..1446
                     /locus_tag="PKN90_01125"
     CDS             394..1446
                     /locus_tag="PKN90_01125"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNZ84918.1"
                     /translation="MKKSHKILIMSMLLISLSNCVTKIPEPPKDIDSDFVEDHTFDTP
                     IVITFNENSVTANPASVSGITFTINEGHIVVNSEHTNVEYILTGNTNNGSFKLYSTSK
                     CKLTLNNVHIVSDNSPAINIQTSKSIFVNIANDTENTLSDKNPYILTELEDAKGTFFS
                     EGQLIFSGTGSLQIASKQAHGIITDDYIRIRSGEIRINEAVKDGFSAKDHILIEGGDI
                     YLSVSDDGISCRQGYITIEDGNITVESKDEGIVAGYDPETDLVIEGVVPDITINGGAI
                     TIKTYGEKGHGIKSVGMITFNGGNTDITTSGIKSDGIDCDGGTVTINGGTLSITARDD
                     CMNNTPVITAGEYTCL"
     gene            1472..1942
                     /locus_tag="PKN90_01130"
     CDS             1472..1942
                     /locus_tag="PKN90_01130"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ84919.1"
                     /translation="MKKIISSILLAYIFVSSGFAMTHMRVEKNDASEEVIQLNTIRTI
                     RFQGENVQFNYKNGNSDLFAANTLKSLQFSSIPSSIEEKTSAELFFYPNPATDYIYLK
                     NLATEEAMISIYQIDGVIVMKGKQSLHTGIYVGNLASGLYLLEVNKQTYKFRKL"
     gene            1939..3144
                     /locus_tag="PKN90_01135"
     CDS             1939..3144
                     /locus_tag="PKN90_01135"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNZ84920.1"
                     /translation="MKKLLILFYTLIFTLNISAQMSIYFQQGEVDVKVPFVDIDSITF
                     NPVESNYLYTQLRIHNVNNTSTDLTLADIDNIYFKDSDILNPADITTQVLISYNGNSI
                     STTNPLAGNGVSISNTGGNVVVTSTVAGVEYILSGTTSNGSFKIYSDFEFKLTLNGAT
                     ITSTFGPAINIQSSKRVFVYLVDGKESTLTDSSNYPDPLTATEDTKGCCFSEGQLIFM
                     GNGKLSVNAKAKHAIVSDDYIRVISGNISVLDAMSDGLKSNDWIIIDNGYVVINAKDD
                     GIQSDKGHIIINGGTVEVTSTDEGIAASYDPLVTGDYTIKPYITINGGDVTVTTTAES
                     AHGIRTGDDITINGGNISITTNGLKSDGMKTEGKITLNGGTAIIHAFSKCMDYGTLID
                     NGNVVTCLY"
     gene            3256..>5111
                     /locus_tag="PKN90_01140"
     CDS             3256..>5111
                     /locus_tag="PKN90_01140"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HNZ84921.1"
                     /translation="MNKKSHSFPLLFNHSIRHRLVAFSILLLLSALFATTHAQPLHIA
                     GSSTYYNELLGGSVTNFGVPLTLENRRNTSVGVKTCSGKATGTGWIKFVQETNSWNTQ
                     WNGGASAFTLSGNTYMATAGKYDVTSSGGGNINVSITKDLFYTFIIGQNTTSDNDVSV
                     LVTAQAPRNVTTMTHSTPQEGQAVTVTSTLNNSITTTSGNGAQYVYLAYSVNGGTYTS
                     TPMTVTATNTVYTGTIPSNVHVADNTISYYTYITTVDGLTPSTTGFDFLCLSQSFVNT
                     YNLNGGTSTPITIKAKRPSSWSNDMYLFFWGGEITSPSWDESSKMNSLGEGWYSFTLP
                     SEITASNIIFRNGITSGLNNQSDDINTVSQSTCYNLSQSGSSKATASQVTCPVIVVGA
                     PTISAHKATVCENNFTDFASLYVSDYGILGSETGYAGGITFLWERSTDNVTWTTFTDG
                     RKATINNIRPTRTGYYRCKMTRFSTSTNSNEDIYSNVIQVTAGSNCTSRTVCSKLPVI
                     VVQTNVESFPTGNFLASNEAFQNAKKKLSVDVKIIWDDTKDDTDNIMTQTDFTNPTRL
                     YYDKKARMNYRGSSSLRYSKKNYAFVVGDDSCTLDGKWIKDTKKMFDINEVN"
BASE COUNT         1806 a          836 c          928 g         1541 t
ORIGIN      
        1 acaccaacta ccgctgggaa tggcgtaaaa ggattagaca ccttgcttga ttttgcagaa
       61 ggattatctc ctcttggcaa cgcaacagct gcagattgtg ctgattattg cgtagtaatg
      121 tttagcgact taactaaaaa agtaactatg cagaatctac ttcacgatgg aggtttttcg
      181 agcatgagta tgagtgaaac cgtaattgaa gattacagaa aaggtttagc agccaacgaa
      241 taaactgaaa agagactatt ttagatagaa cactattttt gttgaaaaac gaaaatagtg
      301 tttttttacg ttaaattcta ttttttttgt ataaaaatgg gaatattagc taaaaaggtg
      361 tatttttgta ttttgttaac cttaaataat tgtatgaaaa aatcacacaa aatacttata
      421 atgagtatgc tacttatctc gcttagtaat tgcgttacca aaattcctga gccaccaaaa
      481 gacattgatt ctgattttgt tgaagatcat acttttgaca ctcctattgt aattacattt
      541 aatgaaaatt ctgttacagc caacccagct tctgttagtg gtattacctt tactattaat
      601 gagggacata ttgttgtgaa ttctgaacac accaatgtag agtatatact aacaggtaat
      661 acgaacaacg gatcatttaa attatacagc acatcaaaat gtaaactaac attaaacaac
      721 gtgcacatag taagtgataa ctcgcctgcc atcaacatac aaaccagcaa aagtatattt
      781 gtaaatattg cgaatgatac tgaaaacaca ttatctgata aaaatccata tatactaacg
      841 gaattagaag atgcaaaagg aacatttttt agcgaaggtc aacttatttt tagcggaaca
      901 ggttctttac aaatagctag taaacaagca catggcatta taactgatga ttacatacgt
      961 atacgaagcg gtgaaatacg tattaatgaa gcagtaaaag atggattcag tgctaaggat
     1021 catattttaa ttgaaggagg agatatttat cttagcgtgt ctgatgatgg tatttcgtgc
     1081 agacaaggct acattacgat tgaagatggc aatataacag ttgaatcaaa ggatgaaggc
     1141 attgttgcag ggtatgatcc tgaaacagac ttggtaatag aaggagtagt tccagacatt
     1201 accataaatg ggggtgctat tacgataaaa acatatggag aaaaaggaca cggaataaag
     1261 tcggtaggaa tgattacttt taacggagga aatacagata taacaacttc tggaataaaa
     1321 tcggacggaa ttgattgtga tggtggcaca gtaacaatca atggaggaac tctctctatc
     1381 acagcaaggg atgattgcat gaataataca cccgttataa cagcagggga atatacttgt
     1441 ttataaaaat taaccacaaa attatacatc tatgaaaaaa attatttcta gtattctgct
     1501 tgcatacatt tttgtatcta gtggatttgc aatgacacat atgcgtgttg agaaaaatga
     1561 tgcatctgaa gaagttattc agttaaacac cattcgcaca attcgttttc aaggagaaaa
     1621 tgttcaattt aactataaaa atggaaattc tgatttattt gcagcgaaca cattaaaaag
     1681 cttacaattt tcttctattc catctagtat agaagaaaaa acttccgctg aattattttt
     1741 ttatcccaat ccagctaccg attatattta tttaaaaaac ttggcaacag aagaagctat
     1801 gatttctatc tatcaaattg atggagttat tgtgatgaaa gggaaacaat ctcttcatac
     1861 aggtatttat gttggcaatt tagcaagtgg actttacctt ttagaagtaa ataaacaaac
     1921 ttataaattt agaaaattat gaaaaagcta cttatactat tttatacact catttttact
     1981 ttaaacatat cggctcaaat gagtatttac tttcagcaag gagaagtgga tgtaaaagta
     2041 ccatttgttg atattgatag cattacgttc aatcctgttg aatctaatta tctttacact
     2101 caattacgta tacataacgt taacaacaca agcacagatt taacattagc agatattgat
     2161 aatatttatt ttaaagatag tgatatttta aatccagcag atataacaac ccaagtcctt
     2221 atttcgtata atgggaatag tatttcaacc accaaccctc ttgcaggaaa cggggtatct
     2281 attagtaaca cagggggaaa tgtagtagtt acatctacag tagcaggtgt cgaatatatt
     2341 ttatctggga caacgagcaa tggatcattt aaaatttata gtgattttga gtttaaatta
     2401 acattaaatg gagctactat aaccagtaca tttggacctg ccatcaatat tcaatcaagt
     2461 aaacgcgtgt ttgtctattt agtagatgga aaagaaagta ctcttactga cagttcaaac
     2521 tatccagatc cattaaccgc aacagaagat acaaaagggt gctgctttag tgagggacaa
     2581 ttaattttta tgggtaatgg taagttatct gttaatgcaa aagcaaagca tgctattgta
     2641 agcgatgatt atatacgtgt tattagcggc aatatttcag ttcttgacgc aatgagcgat
     2701 gggttaaaat caaatgactg gataatcata gacaatggat atgtagttat aaatgcaaaa
     2761 gacgatggca tacaatcaga taaaggtcat atcattatta atggtgggac agtagaagtt
     2821 acaagcacag atgaaggaat agctgcatcc tatgacccat tagtaacagg ggattacaca
     2881 attaagccct atattactat taatggagga gatgttaccg taactacaac agctgaatca
     2941 gcacatggca tacgaacagg tgatgatatt acaattaatg gaggaaatat ctctattact
     3001 acgaatgggc tcaaatctga cggaatgaaa acagagggta aaattacttt aaatggtgga
     3061 acagctatta ttcacgcatt ttctaaatgt atggattatg gtacgcttat tgataacgga
     3121 aatgtagtaa cttgtctcta ctaatatttt cacatctact tgcctactat cataggaaac
     3181 tcacattcgt gtgatagtag gtatatttct ttcaacatat ttacatttat ttctctaacc
     3241 ttataactac gtattatgaa caaaaaatca catagttttc cattgctatt taatcattct
     3301 attcgtcatc gcttagtagc tttttccatt ttattactgc taagtgcctt gtttgccact
     3361 acccatgcac aacctctaca catagctggg agttcaacgt attataatga attattgggt
     3421 ggttctgtta caaactttgg tgttccgtta accctagaaa atcgccgaaa taccagtgtt
     3481 ggtgtcaaaa cctgctctgg aaaggcaact ggtacaggtt ggattaagtt tgtacaagaa
     3541 accaacagtt ggaatactca atggaatgga ggtgcatcag cgtttacact ttctggcaat
     3601 acatatatgg ctactgctgg caaatatgat gtaacgtcaa gtgggggagg aaatattaat
     3661 gtaagtataa caaaagactt gttttacacc tttataattg gtcaaaatac aacgtctgat
     3721 aacgatgtgt ctgtattagt aactgcacaa gccccgcgga atgttacgac aatgacacac
     3781 tctactccac aagaaggaca agcagtaaca gttaccagca cactcaataa ttccataacc
     3841 actacttcgg gtaatggagc acaatatgta tatctagcat acagtgttaa tggtggaaca
     3901 tacacatcta caccgatgac agtaactgca acaaacacgg tatatacagg aacaatcccc
     3961 tcaaatgttc acgtagctga taacaccatt tcgtattaca cctatattac cactgtggat
     4021 ggattaaccc catctactac aggctttgat tttctttgcc ttagccaatc ttttgtaaat
     4081 acatacaacc ttaatggagg aacatctacc ccaataacta tcaaagccaa acgtccaagc
     4141 tcttggagca atgatatgta tttatttttc tggggaggtg aaataacttc tccaagttgg
     4201 gatgagtcta gcaaaatgaa tagcctcggt gaggggtggt attcatttac tttgccctct
     4261 gagattacag ctagtaatat tatatttaga aatgggataa cctcagggtt aaataaccaa
     4321 tcagatgata tcaatacagt atcacaatca acatgttata atctgagtca aagtggatct
     4381 tcaaaagcaa ctgcttcgca agtaacatgt cctgttattg ttgtgggagc acctaccatt
     4441 tcagcacata aagctacagt atgtgaaaat aattttacgg attttgcatc tttatatgta
     4501 agtgattatg gcatactagg ttcagaaaca ggatatgctg gcgggattac gtttttatgg
     4561 gaacgttcaa cagacaacgt tacttggacg acgtttaccg atggaagaaa agctacgata
     4621 aacaacatta gaccaactag aacaggctat taccgttgta aaatgacacg tttttctacc
     4681 tcaacaaata gcaatgagga tatttatagc aatgtcatac aagttacagc aggatcaaac
     4741 tgtacatcac gtactgtttg tagtaaattg cctgtaatcg ttgttcaaac aaatgtagag
     4801 agttttccaa ctggcaactt tttagcatca aatgaagctt ttcagaatgc taaaaagaaa
     4861 ctaagtgtag atgtaaaaat catttgggac gacacgaaag atgatacaga taatatcatg
     4921 acacaaacag actttactaa ccctactcgt ttgtattacg ataaaaaggc acgcatgaat
     4981 tatcgtggat catctagttt aaggtattca aaaaagaact acgcatttgt tgtaggagat
     5041 gatagttgta cattagatgg aaaatggata aaagacacaa aaaaaatgtt tgacatcaat
     5101 gaagtcaaca a
//
LOCUS       DAOPUR010000038         5103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_16412_length_5103_cov_3.507132, whole genome shotgun sequence.
ACCESSION   DAOPUR010000038 DAOPUR010000000
VERSION     DAOPUR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16412_length_5103_cov_3.507132"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PKN90_01145"
     CDS             complement(<1..876)
                     /locus_tag="PKN90_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNZ84922.1"
                     /translation="MENKEVILITGSKGLIAKQLASSLASQYTIRLLTRKPTKPNEYC
                     WDIHQQFIDEKALENVSHIIHLAGAGIADKHWSKSRQKEIVSSRVDSAKLLLTACIKQ
                     NSKLKSFISASAIGYYGQTSSEHIFTETDEPGNDFVSSVCVAWEKAADEFEQNGIAER
                     VIKLRTGIVMATEGGALPNILFPLQLRVALVLGNGKQYLPWIHLHDICSMYQFAIENK
                     HCKGVYNCVANESITYESCIQTAKNMYSKKMLPFSVPAFLLKFVLGKRSSLLLYGSRI
                     NNARIIESGFSFSYTT"
     gene            complement(881..2368)
                     /gene="crtI"
                     /locus_tag="PKN90_01150"
     CDS             complement(881..2368)
                     /gene="crtI"
                     /locus_tag="PKN90_01150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNZ84923.1"
                     /translation="MKNKEVIVVGAGLGGLTTALRLVKKGYKVTILEKNTQAGGRLNR
                     IQKDGFTFDTGPSFFSMSYEFKKLAEECNITMPFEFVELDPLYSVHFAGDPRSYHLYK
                     DMKKLAEQFKDIEPDFEAKMATYLQKSGALFHDTIDLVVKNNFDSIFEYIATLMRVNP
                     VHLPVLLRNFWQQVGLHFSSKEVKRIVSLVAFFLGRTPFDTMAIYTLLSYTEFKHDGY
                     YNVKGGMYKIVEGIVAELEKENVQFVYNTEVVNIQSEKIRVKSVIDQHGKEWLADIFV
                     INADAAVFRGTVLKRKKFSTKKLDKMNWTMGYLTIYLGIDCKLPQVNHHNYYLGTNYE
                     EYANNVMKNPDTLDKPYYYVNVLSKHNEECAPAGCESLFFVCPVPTLLQKPNWDDRDK
                     IVDSIIADFSNRIDKNIAPHIVSKTIFTPKEWETMINLHRGSGLGLSHTMFQIGGFRP
                     ANFDEKFENTFYVGASTIPGAGLPMAVISSELVCKRVYAFTEKNN"
     gene            complement(2389..3003)
                     /locus_tag="PKN90_01155"
     CDS             complement(2389..3003)
                     /locus_tag="PKN90_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84924.1"
                     /translation="MSEWKKIIDTMEKTSLTNNQTRLELLNYQYGYIAWCLGQKKHDE
                     ATIYLRRAEKHIDALDNKKYKPADLHAYKAAFYGYKIAITPFKASYLGPKSIWHVKKA
                     LEIEPENMFALLQYGNIYYYMPVTFGGSKETASQYYLKVEKWYEKHPQQRITNWNYIN
                     VLVTLTNTYIALGKKDKATEYYNKIVTAEPTSSWIKQEIYPQLK"
     gene            complement(3081..4172)
                     /locus_tag="PKN90_01160"
     CDS             complement(3081..4172)
                     /locus_tag="PKN90_01160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ84925.1"
                     /translation="MIYFAYIVLGFALLQLLVVGVNWIFRQSLTQEYTHNDKLVSVLI
                     PARNEEKNIASILGDLRKQTYQHIEIIVLDDDSTDNTSSIIEQQIQHDSRVKLLASKG
                     LPKGWLGKNYACHQLAKEAKGEYLLFLDADVRIQPRLIGKLLHFSINKQKQLVSIFPK
                     QEMKSAGEWKTVPLMNYILLTLLPLILVRFPYFSSLSAANGQCMFFSQNSYQSILPHQ
                     QLKNTAVEDIEIARLYKKRKQHIACLASVDDISCRMYSNNDEAITGFSKNCLSFFGGS
                     AILGLLFWALTSLSFIVLLWFPILLAIYLMMVISTRIGVSVISHQSVIKNLQYLLYQH
                     IYLGILLFSAIKNKRKRHLLWKERNIYHS"
     gene            complement(4169..4798)
                     /locus_tag="PKN90_01165"
     CDS             complement(4169..4798)
                     /locus_tag="PKN90_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ84926.1"
                     /translation="MIKAKHHWFIYPFFIFYSFWKIKRHFKDVHINTLLPTDTIDKPI
                     LLIGNHISWWDGFIVQYINQKLWKKKFHFMMLEEQLKKHQFLNLTGGFSINKKSKEII
                     ASLTYAQELLHTKNNLVLMFPQGKITSMHQQTMVFEKGIERILQHASDTIEVVFMVNI
                     IDYFSDEKPTLYIYLTNYQTDKPNHKDIEKAFQDFYNSCLGTQQKRATE"
     gene            complement(4813..>5103)
                     /locus_tag="PKN90_01170"
     CDS             complement(4813..>5103)
                     /locus_tag="PKN90_01170"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNZ84927.1"
                     /translation="TAFMVDKTKWHAVLKIVVAASGMLVYDIVLEQVAPKMDMWSWQN
                     NVVPIQNYIAWFVIAAFFHSLLKIFKVKLSNPLAAHIIACQFVFFVVLLFLL"
BASE COUNT         1536 a          925 c          854 g         1788 t
ORIGIN      
        1 cgtagtgtag gaaaaagaaa acccggattc aatgattcgt gcattattta ttctgctacc
       61 gtaaagtaat aaggaagaac gtttgccaag tacgaatttt agtaaaaatg cagggacaga
      121 aaaagggagc attttttttg agtacatgtt ctttgcagtt tgaatacaac tctcataagt
      181 aatagattcg ttagctacgc aattatacac acctttgcag tgcttattct ctatcgcaaa
      241 ttgatacatc gaacatatat cgtggagatg aatccatggc aaatattgtt ttccgttgcc
      301 aagcaccagt gctacacgta gttgtaatgg aaataatatg tttggtaagg caccgccttc
      361 tgttgccatc acaatgcctg tgcgtagttt tattacccgt tctgctattc cgttttgttc
      421 aaactcatcg gctgcctttt cccaagctac acatacagaa ctaacaaaat catttccagg
      481 ctcatctgtt tcagtaaaaa tatgttcgga gcttgtttga ccatagtaac caattgccga
      541 agccgaaata aatgatttca acttactgtt ctgtttgata caagccgtaa gtagcagttt
      601 ggcactatct actctgcttg atactatctc tttttgtcgc gatttactcc aatgtttgtc
      661 agcaatacca gcacccgcta aatggatgat gtgagataca ttctcaagtg ctttttcatc
      721 aatgaattgt tgatggatgt cccagcagta ttcattcggt tttgttggtt tgcgagttaa
      781 gaggcgaatt gtatattgac tagccaaaga actcgctaat tgcttagcaa ttagcccctt
      841 acttccagtt attaaaatta cctctttgtt ttccatgtaa ctaattattt ttttccgtaa
      901 aggcatatac gcgtttacat actaattctg aacttattac agccatgggc aatcctgctc
      961 ctggtatggt tgaagcacct acataaaagg tattttcaaa tttttcgtca aaattggcag
     1021 gtctaaaccc tccaatttga aacatcgtgt gcgacaatcc caatccgcta ccacgatgaa
     1081 gattaatcat tgtttcccat tctttaggcg taaaaatagt tttagacact atatgtggag
     1141 caatgttttt gtcaatacga ttagaaaaat ctgcaataat gctatcgact attttatcac
     1201 ggtcgtccca atttggtttt tgcaataggg taggtactgg acatacaaag aataaacttt
     1261 cgcaacctgc aggagcacat tcttcgttgt gcttggatag tacgtttaca tagtaataag
     1321 gcttatctaa tgtatctggg tttttcatca cattgttggc atattcttcg taattagtgc
     1381 cgaggtaata attatgatga ttcacctgag gaagcttgca atcaatgcct aaataaattg
     1441 ttaaataacc cattgtccaa ttcattttat ccagtttttt tgtgctgaat tttttacgtt
     1501 tcaataccgt acctctaaat acagcagcat cggcatttat gacaaaaata tcggcaagcc
     1561 attctttgcc atgttgatca attacgcttt ttacgcgtat tttttctgat tgtatattca
     1621 caacctcggt attgtacaca aactgtacgt tttctttttc taattctgca acaatacctt
     1681 ctacaatttt atacatacct ccttttacgt tgtaatagcc atcgtgttta aattcagtat
     1741 acgataatag ggtgtaaatt gccatggtat caaaaggagt tctgcccaaa aagaatgcta
     1801 caagcgatac aatgcgttta acttctttcg atgaaaaatg taagcctact tgttgccaaa
     1861 agttacgtag caataccggt aagtgtactg ggtttacacg cattaaggtt gcaatatatt
     1921 caaatataga gtcaaagttg ttttttacca ccaagtcgat tgtgtcatga aacaatgcac
     1981 ctgatttttg caaatacgta gccattttag cttcaaaatc aggttcgata tctttaaatt
     2041 gttcagccaa ttttttcata tctttgtaca aatgatagct acgtgggtcg ccagcaaagt
     2101 gtacgctata caaggggtcg agttctacaa attcaaaagg catcgttata ttacattctt
     2161 cagctaattt tttaaattca tacgacatac taaaaaaact tggacctgtg tcgaacgtga
     2221 aaccatcttt ttggatgcga tttaatcttc caccggcttg tgtgtttttt tctaatatgg
     2281 ttactttgta gccttttttt actaaacgta atgctgtggt taatccccct aatccagcac
     2341 ctacaacgat aacttcttta tttttcatat accaatattc tttgattttt attttaattg
     2401 cggataaatt tcttgtttaa tccaagatga tgttggctct gccgttacta tcttgttgta
     2461 atactctgtt gctttatctt tttttcctag agcaatatac gtgtttgtta atgttactaa
     2521 tacattgata tagttccagt tagtaatacg ttgttgtgga tgtttttcgt accatttttc
     2581 tactttcaag taatactggc tagctgtttc ttttgatccg ccaaatgtta caggcatata
     2641 atagtaaata tttccatatt gcaataacgc aaacatattt tcgggttcaa tttccaatgc
     2701 cttttttaca tgccaaatgc tttttggtcc taaataactt gctttaaaag gtgttattgc
     2761 aattttatat ccataaaatg ctgctttata agcatgtaaa tcagcaggtt tgtatttttt
     2821 attatccaat gcgtcaatgt gtttctcggc tcgtcgcaaa tatatggtag cttcatcgtg
     2881 ttttttttgt cctaagcacc atgctatgta cccatattgg tagttgagaa gttcgagtcg
     2941 tgtttgattg ttcgtcaaag aggttttctc catggtatca attattttct tccactcgct
     3001 catagtgttg ttaacgtaag caaggtacac tttttcttgg ttggaattag ccagacagct
     3061 aagtgtaata aataatgaaa ttaggagtga tatatatttc tttctttcca tagtaaatga
     3121 cgtttgcgtt tgttttttat ggcactaaac aataaaatgc ctaaataaat atgctgataa
     3181 agaaggtatt gtaagttttt aataacagac tgatgactaa taacagacac gccaatacgt
     3241 gtactaataa ccatcattaa atagattgcc aataagattg gaaaccataa tagaacaata
     3301 aaagacaaac ttgtaagagc ccaaaatagc aatccaagta tagccgaacc tccaaaaaag
     3361 ctcaagcaat ttttgctaaa accagttatt gcttcatcgt tgttactata cattctacac
     3421 gaaatatcgt caactgaagc taagcatgct atatgttgtt ttcgtttttt gtataaacgt
     3481 gcaatttcaa tatcttcaac cgcagtattt ttgagttgtt ggtgtggtaa aattgactga
     3541 taactatttt gcgaaaaaaa catacattgc ccattagctg ccgataatga tgaaaaataa
     3601 ggaaaccgaa ctaaaatgag cggtagtaaa gtaagcagta tgtaattcat taaaggaaca
     3661 gttttccatt ctccagcact tttcatctct tgtttcggaa aaatggatac taattgtttt
     3721 tgtttgttga tactaaaatg cagcaattta ccaatgagcc ttggctgaat gcgtacatct
     3781 gcatctaaaa aaagcaggta ttctcctttt gcttcttttg ccaattgatg acaagcatag
     3841 ttttttccta accagccttt tggtagacct ttcgatgcta gcagttttac tcgcgaatcg
     3901 tgctgtattt gttgctcaat aattgaggac gtattatcag tcgaatcatc atcaagcact
     3961 ataatctcta tgtgttgata ggtttgtttg cgtaaatcgc ctaaaataga tgctatgttc
     4021 ttttcctcgt ttcgtgcagg aatcagcaca gataccaatt tgtcattatg ggtgtattct
     4081 tgtgtcaaac tttgacgaaa tatccagttt acacctacca cgagcagttg taacaaagca
     4141 aaaccaagca ctatgtaggc aaaataaatc attccgtagc acgtttttgt tgggtaccta
     4201 aacaactgtt gtaaaaatct tgaaaggctt tctcaatgtc tttatgattt ggtttatctg
     4261 tttgataatt agttaaatag atatataggg taggcttttc atcagaaaaa taatcaatta
     4321 tatttaccat aaatactacc tcaatggtat cgcttgcatg ctgtaaaatt cgttcaatgc
     4381 ctttttcaaa aaccatcgtt tgttggtgca tgctggtaat ttttccttgc ggaaacataa
     4441 gtaccaaatt gttttttgta tgcaataatt cttgtgcata cgtaagtgat gcaataatct
     4501 cttttgattt cttattaata gaaaaacctc cagttaagtt caaaaattga tgttttttca
     4561 attgctcttc taacatcata aaatggaact tctttttcca taatttctga ttgatatatt
     4621 gcacgataaa tccatcccac caacttatat ggttgccaat gagtaagata ggtttatcaa
     4681 tagtgtccgt tggtaaaagc gtattaatgt gcacatcttt aaagtgcctt tttattttcc
     4741 aaaaagaata gaaaataaaa aatgggtaga taaaccaatg atgctttgct tttatcattt
     4801 ttttatccac atttaaagta aaaataacaa tacaacaaaa aatacaaatt ggcacgctat
     4861 aatatgggca gccagcggat tgctgagttt tactttgaaa attttaagca aactatgaaa
     4921 aaaagcagca atcacaaacc atgctatgta gttttgtatt ggcaccacgt tattttgcca
     4981 cgaccacata tccattttgg gagccacttg ttctaacacg atatcataca ccaacattcc
     5041 agatgctgca actactattt tgagtactgc atgccatttc gttttatcga ccatgaaagc
     5101 ggt
//
LOCUS       DAOPUR010000039         4938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_17142_length_4938_cov_3.866271, whole genome shotgun sequence.
ACCESSION   DAOPUR010000039 DAOPUR010000000
VERSION     DAOPUR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17142_length_4938_cov_3.866271"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..832)
                     /locus_tag="PKN90_01175"
     CDS             complement(119..832)
                     /locus_tag="PKN90_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84928.1"
                     /translation="MPYRRLPNTDAARLRALRAVACYKNSPIDTELPFDRRILQEICS
                     FLPQFENAMFEYKQAINSEGNKNNKYQQYIKNVRLYISHFIQVLNLACIRNEIKPEKK
                     LLYKLLPDNYAVPDLSTETLLIEWGKNIIQGENERTRAGGSPIYNPTIAKVQVHYDIF
                     KEAYHSKKVAQKNVGRYAENMQKMRMRADEIILDAWNQIEQAFVHYGMDEMQQKAKEF
                     GIVYYLRRKERQAMTKKSH"
     gene            complement(902..1876)
                     /locus_tag="PKN90_01180"
     CDS             complement(902..1876)
                     /locus_tag="PKN90_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNZ84929.1"
                     /translation="MKSREEIIEIVQNELTLTNWLSEPIDLYKPIAYTMSMGGKHIRS
                     AITLMACNLYSDNIHLALQPAIGIEVFHNFTLLHDDIMDKAETRRGKPTVHVKWNDNT
                     AILSGDAMLIKAYQLLAKCPPENLSEILSVFSQTAIEVCEGQQYDMNFEHQDTVSEEE
                     YIEMIRLKTAVLLAASLKIGAIIGGATPLQANALYDFGIHIGIAFQLQDDLLDVYGDP
                     TIFGKKIGGDIVCNKKTFLLIRALKNANNTQRNKIQHWMTQHPDDPTEKIVQMTSIFN
                     ETGVKQACQQRMKEHYNKALLALNKACEVEEKIVHLQKLASELMQREK"
     gene            complement(1900..2883)
                     /locus_tag="PKN90_01185"
     CDS             complement(1900..2883)
                     /locus_tag="PKN90_01185"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNZ84930.1"
                     /translation="MKKKIAIIAGGNSSEVIVSLKSAQGIYSFIDKKTYEVYIVTLIE
                     KKWSVKLEDETEVDIDKNDFSFIVNGEKILFDFAYITIHGTPGENGILQGYFDLIGLP
                     YSCCGVLAAAITFNKFTCNQYLKGFGIPVAPSILLRKNEHIQNTVVVEKIGLPCFIKS
                     NVGGSSFGVTKVKEEKQIQPAIEKAFAEGNEVIIEGFLQGTEVTCGMYKTHEKTQVFP
                     ITEVVSANEFFDFDAKYNGQVTEITPARIPQEVTNHIQQLTNQLYDILGCKGIIRADF
                     IINKQNVPYLLEVNTTPGMTPTSFIPQQVKAAGMAISSVMEDIIENKLNHL"
     gene            3117..>4938
                     /locus_tag="PKN90_01190"
     CDS             3117..>4938
                     /locus_tag="PKN90_01190"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HNZ84931.1"
                     /translation="MSEEEKKENGGYGASSITVLEGLEAVRMRPAMYIGDIGMKGLHH
                     LVYEVVDNSIDEALAGHCKHIEVFINEDDSITVKDDGRGIPVDMHEKEGKSALEVVMT
                     VLHAGGKFDKDSYKVSGGLHGVGVSCVNALSDYMRVEVHRGGKVYEQEYSCGKPQFSV
                     REIGVSTQKGTTVTFKPDGSIFMTTEYKYDILAGRLRELAFLNAGIRLILTDKRERFE
                     DGSYKSEEFYSTEGLAEFVRFIDQAREKLIDEVIHISTDKYATPVEIAMLYNTSYNEN
                     IHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEENKLLEKLEKNKIEIDGNDFREGLT
                     AVISIKVAEPQFEGQTKTKLGNNEVMGSVDMAVSEALGNYLEEHPKEARTIVEKVILA
                     ATARHAARKARDLVQRKSPLSGAGLPGKLADCAEKDPAKCELFLVEGDSAGGTAKQGR
                     DRATQAILPLRGKILNVEKAMPHKVFESEEIRNIYTALGVTIGTEEDSKALNLSKLRY
                     HKVIIMTDADVDGSHIATLILTFFFRHMKDLIENGYVYIATPPLYLCRKGKIEEYCWD
                     EKQRLAFVEKYGKGSEGGIHIQRYKGLGEMNAQQLKQTTMD"
BASE COUNT         1490 a          763 c         1008 g         1677 t
ORIGIN      
        1 tattagtcta taaaaaagac tcaaaacgac cattttggtg ttcaaaagtc acaaaatacg
       61 gtgcaaattt aacttatata ttccacaaaa aaaaataaac acgccaaaag ttttcttttt
      121 agtgcgattt ttttgtcatt gcttgtctct cttttcttct taagtaatag acaattccaa
      181 attcttttgc tttttgttgc atctcatcca taccataatg aacaaaagct tgttcaattt
      241 ggttccaagc atcaaggatg atttcgtcag ctcgcattct cattttctgc atattttctg
      301 catatctccc tacatttttc tgtgctactt tttttgagtg atatgcttct ttaaaaatat
      361 catagtgtac ttgtactttt gcgatggtcg gattgtaaat aggagaacct ccagcacgtg
      421 ttcgttcgtt ttctccttgt ataatatttt tcccccattc tataagcaaa gtttctgtgc
      481 ttaaatcagg cacggcataa ttgtcgggca atagcttata caataatttt ttttcaggtt
      541 ttatttcgtt acgaatacac gctaaattga ggacttgaat aaaatgggaa atatataatc
      601 tcacattttt tatgtattgt tggtatttgt tgtttttgtt tccttcgcta tttatggctt
      661 gtttgtattc aaacatagca ttttcaaatt gaggcaaaaa gctacaaatt tcttgtagta
      721 ttcgtctatc gaaaggcagt tcggtgtcga taggcgaatt tttgtagcaa gcaacagccc
      781 gtagtgctct caaacgtgca gcatctgtat ttggaagtct tctgtagggc ataaggttaa
      841 caaaaaatat gataaacgca aatgtacgtt gtatttttgt aaaatccaaa ttcgtattcc
      901 attatttttc cctttgcatt agttctgatg ctaatttttg taaatgtact attttttctt
      961 ctacttcgca tgctttattc aaagcaagta gggctttgtt ataatgctct ttcattcgtt
     1021 gttggcaggc ttgttttaca cccgtttcgt tgaatattga ggtcatttgt acaatttttt
     1081 cggttgggtc atcaggatgt tgtgtcatcc agtgttggat tttattacgt tgagtattgt
     1141 ttgcattttt cagtgcacga atcaacaaaa aagttttttt attgcagaca atatctccgc
     1201 cgattttttt tccgaaaatt gtaggatctc catatacatc aagtaagtcg tcttgtagtt
     1261 gaaaggcgat gccaatatga ataccaaaat cgtataaagc attcgcttgt agtggagttg
     1321 caccaccgat aatagcacca atttttaagc ttgcagccaa taatacggct gtttttaaac
     1381 gaatcatttc aatgtattct tcttctgaaa ccgtatcttg atgttcgaaa ttcatatcgt
     1441 actgttgtcc ttcgcaaact tcaatagctg tttgagaaaa tacagaaagt atttcagaaa
     1501 ggttttctgg tgggcatttt gccagtagtt gataggcttt aatgagcatg gcatcgcccg
     1561 acaagatagc ggtgttatcg ttccatttta catgtacggt tggcttacct ctacgggttt
     1621 ctgctttatc cataatgtcg tcgtgcaaaa gcgtaaaatt atgaaaaact tcaattccaa
     1681 tggcaggttg aagagccaga tgtatattgt cgctgtatag gttgcaagcc atcaatgtga
     1741 ttgcagagcg tatatgtttt cctcccatag acatcgtata ggctatgggt ttatataagt
     1801 ctataggttc tgatagccaa ttggtaagag ttagttcgtt ttgtactatt tcgataattt
     1861 cttctctgct tttcatgccg aatgactgtt aaatatacat tataggtgat ttaatttgtt
     1921 ttcaataatg tcttccatta ccgatgaaat agccatacca gctgctttaa cttgttgagg
     1981 tataaagctt gtaggggtca ttccaggtgt agtgttgact tcgagtaaat aagggacatt
     2041 ttgtttgttg ataataaaat ctgctcgaat gattcctttg catccaagta tatcgtatag
     2101 ctgatttgtt aattgttgta tatggtttgt aacttcttga gggatacggg ctggagtaat
     2161 ttctgttact tgaccgttgt atttggcgtc aaaatcgaaa aattcgtttg cactaacgac
     2221 ctctgtgata ggaaatacct gtgttttttc atgtgttttg tacattccgc aggttacctc
     2281 tgttccttgt aagaatcctt caataataac ctcattgcct tctgcaaaag ctttttcgat
     2341 agcaggttga atttgctttt cttcttttac tttggttaca ccaaagctgc taccacctac
     2401 atttgatttg atgaaacaag gtaagccgat tttttcgaca accacagtgt tttgtatgtg
     2461 ttcgttttta cgtagcaaaa tagaaggggc tacaggtatg ccaaatcctt ttaggtattg
     2521 attgcacgta aatttattga atgtaatagc agcagccaat actccacagc aggagtaggg
     2581 taatccaatt aaatcaaaat atccttgtaa aatcccattt tcaccagggg ttccgtgtat
     2641 ggtgatatag gcaaaatcaa aaagtatttt ttctccattt actataaaag aaaagtcgtt
     2701 tttgtctatg tcgacttctg tttcatcttc taatttgaca gaccattttt tctcaattaa
     2761 ggtaacaata tatacttcgt aggttttttt atcaataaaa gagtaaatac cttgagcact
     2821 tttaagagaa acgataactt cggaagagtt tcctccagca atgatagcaa tctttttttt
     2881 cataagtaaa tattgaagct aatgatataa actaaataat actatgttgg ttataaggca
     2941 aagacataag aaagtgcaaa tatacaagga ttttttttta tttgattgat gaataagaga
     3001 ataaaaaagg gaaaatctaa attaccattt tagagtcatt ttgtgtctgt aaaatttcgc
     3061 tttcttgata agtttatgta aatttgtctg tttatttttt acttaattta aaaattatga
     3121 gcgaagaaga aaaaaaagaa aatggtgggt atggagccag tagtattact gtattagaag
     3181 ggttggaggc agtacgtatg cgtccggcta tgtatattgg tgatattggg atgaaggggt
     3241 tgcatcacct tgtatatgag gtggttgaca attcaattga cgaggcactt gctggccatt
     3301 gtaaacatat tgaagtattt attaacgaag atgattcgat tactgtaaaa gatgatgggc
     3361 gtggtattcc tgttgatatg catgaaaaag aaggtaagtc tgctcttgag gttgtaatga
     3421 cagtattgca tgctggtgga aagtttgata aagattcgta taaagtatct ggggggttgc
     3481 atggagtagg tgtttcttgt gtaaatgcct tgtctgatta tatgagggta gaggtacata
     3541 gagggggcaa ggtttatgag caggagtact cgtgtggtaa accccaattt tctgtcagag
     3601 aaattggcgt gtctactcag aaaggaacta ctgtaacctt taagccagat ggatctattt
     3661 ttatgactac tgaatataaa tatgatattt tagctggtcg tttacgtgag ttagcatttt
     3721 taaatgcagg tatccgtttg attttgacag ataaacgtga gagatttgaa gatgggtcgt
     3781 acaaatcgga agaattttat tcaacagaag gattggcaga atttgttaga tttatagatc
     3841 aagctcgtga gaagttaatt gatgaagtca ttcatatatc aacggataaa tatgcaactc
     3901 cagtagaaat agctatgcta tacaatactt cttacaatga aaatatccat tcatatgtaa
     3961 ataatatcaa tactattgag gggggaactc atttggcagg ttttagacgt ggattgaccc
     4021 gtacattaaa gaaatatgca gaagagaata aattgctcga aaagcttgaa aaaaataaaa
     4081 tagaaataga cggaaatgat tttagagagg gcttaacggc ggttatctca attaaggtag
     4141 cggagccaca gtttgagggt cagacgaaaa ccaagcttgg taataatgag gtgatggggt
     4201 cggtggatat ggctgtaagc gaagcattgg gaaattattt agaggaacat ccaaaagaag
     4261 cccgcaccat agtcgaaaaa gttattttag ccgccacagc ccgtcatgca gctcgtaaag
     4321 ctcgcgattt agtacaacga aaatcgccat tatcgggagc aggattgcct ggtaaattag
     4381 ccgattgtgc cgaaaaggat ccagcaaaat gcgaattgtt tttagtagag ggagattcgg
     4441 caggtggtac agccaaacaa ggtagagatc gtgctacaca ggctattttg cctttgcgtg
     4501 gaaaaatatt aaacgtagaa aaagccatgc cccacaaggt gtttgagagc gaagaaattc
     4561 gtaatattta tactgctttg ggagttacca ttgggacaga agaagatagc aaagcactaa
     4621 atttatcaaa attacgttat cataaagtga ttatcatgac cgatgctgat gttgatggta
     4681 gtcatattgc tacgctaatt ttaacgttct ttttccgaca tatgaaagat ttgatagaaa
     4741 atggatatgt gtatattgct acacctccct tatatttgtg tagaaaagga aagattgaag
     4801 agtactgttg ggatgaaaag caacgtttgg cttttgtaga aaaatatggc aaggggagtg
     4861 aaggtggtat ccatatacaa cgctacaaag gtttgggaga gatgaatgct caacaattga
     4921 aacaaacaac aatggacc
//
LOCUS       DAOPUR010000040         4921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_17217_length_4921_cov_2.713317, whole genome shotgun sequence.
ACCESSION   DAOPUR010000040 DAOPUR010000000
VERSION     DAOPUR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17217_length_4921_cov_2.713317"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKN90_01195"
     CDS             complement(<1..630)
                     /locus_tag="PKN90_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNZ84932.1"
                     /translation="MQLIDGKAIADIIKQEIAVEVATIKSMGQRPPHLAAILVGHDGG
                     SETYVAHKVKACEQCGFNSTLIRYEDTVTETELLAKISELNNDTAIDGFIVQLPLPKH
                     ISEQKIIEAIDYRKDVDGFHPINVGRTSIGLPAFVSATPAGIMELLKRYGIATAGKHC
                     VVLGRSNIVGKPVANLMIQKAYPGNATVTVCHSYSADLVEQCQKADIIIA"
     gene            complement(638..1951)
                     /gene="ffh"
                     /locus_tag="PKN90_01200"
     CDS             complement(638..1951)
                     /gene="ffh"
                     /locus_tag="PKN90_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNZ84933.1"
                     /translation="MFENLSERLERSFKILKGQGKITEINVAETLKDVRKALLDADVN
                     YKIAKTFTDTVKEKALGMNVLTAIKPNELMVKIVRDELALLMGGTSVDINTKGSPAVI
                     LMSGLQGSGKTTFSGKLAKMLKSKKGKNPLLAACDVYRPAAIEQLKVLGEQIGVPVYS
                     EIENKNPIQIAQNAIAFAKKNGHDLLIIDTAGRLAIDEQMMNEIAAIKQAIQPQEILF
                     VVDSMTGQDAVNTAKEFNDRLDFTGVVLTKLDGDTRGGAALSIRSVVDKPIKFVGTGE
                     KMEAIDQFHPERMADRILGMGDIVSLVEKAQEQYDEEEARRVAKKIAKNQFDFNDFMG
                     QIQQIKKMGNLKDLAAMIPGMGKAMKDVDIKDDAFKGIEAMIQSMTPAERENPQLLNG
                     TRKMRIANGSGTSIQEVNKLIKQFDGIRKMMRMVQTNGNKFPKFK"
     gene            2175..3962
                     /gene="lepA"
                     /locus_tag="PKN90_01205"
     CDS             2175..3962
                     /gene="lepA"
                     /locus_tag="PKN90_01205"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNZ84934.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEETSTVTGKEMQAQVLDNM
                     DLERERGITIKSHAIQMNYIYEGEKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ATQGIQAQTISNLYMAIEHDLEIIPIMNKMDMDSAMPEEVEDKIVELLGCKSDEIIKA
                     SGKTGLGVQEILQAIVKRIHPPKGDPEAPLQCLVFDSVFNSFRGVITYMKIENGTIRK
                     GDLVKFFNTGKEYFADEIGVLRLGMEPREELRCGDVGYIISGIKVSKEVKVGDTITHV
                     KRPCEKGIEGFEEVKPMVFAGIYPIDSEDFEDLRASMEKLQLNDASLTFEAESSAALG
                     FGFRCGFLGMLHMEIVQERLDREFDMDVITTVPNVQYKIYTRKNEVLDVHNPSGMPDI
                     TLIDRIEEPYIRASIITRTDFIGSIMSLCLGKRGELIKQDYISSNRIELIYDMPMGEI
                     VFDFYDKLKSISKGYASFDYHISGFRPSKLVKMDILLNGESVDALSSLTHFDNAVTIG
                     RRMCEKLRELIPRQQFDIAIQAAIGSKIIARETIKAVRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMKQIGSVEVPQKAFMEVLKLD"
     gene            4011..>4921
                     /locus_tag="PKN90_01210"
     CDS             4011..>4921
                     /locus_tag="PKN90_01210"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNZ84935.1"
                     /translation="MEHVSDVAQHVAMPIWASAPFALMLIMIAIGPLLWEHWWEQNKN
                     KLLVSFLLSVPTVIFLIINGFSHDLEHQIMFDYVPFIILLTSLYVISGGLFLTGDIQA
                     KPKINTLFMAIGYVLASIMGTTGAAMLLIRPLITTNSQRMYKTHTILFFIAAVANAGG
                     LLTPLGDPPLFLLYLRGADFGWFLHMLPEWLFIGTVLIGLYYLLDRRYYKKEKTEAIE
                     SDNANVEPLRIGGSKNFFLLIGVILCVAFVNVNYIPEMGNPHGSLWIKYLREIILIGF
                     ILVSMDITKKKTRELKGFSWTPIYEVAA"
BASE COUNT         1507 a          908 c          881 g         1625 t
ORIGIN      
        1 cgcaattata atatctgctt tttggcattg ctccactaaa tcagccgaat agctatgaca
       61 taccgtaacc gtagcattgc ccggatacgc cttttgaatc attaaatttg ccacaggttt
      121 gcctacaata ttacttcttc ctagcaccac acaatgtttg ccagcagtag caattccata
      181 acgtttaagc aattccatta taccagctgg tgtagccgac acaaatgcag gcaatccaat
      241 agaagtgcgt cctacattaa ttggatgaaa accatctaca tctttgcgat aatcaatggc
      301 ttcgataatt ttttgttctg aaatatgttt tggcaaaggc aactgtacaa tgaatccatc
      361 tatggcagta tcgttattta attcactaat ttttgccaac aattcagttt cagtaacggt
      421 atcttcgtat cgtatcaggg tagaattaaa tccacattgc tcgcaagctt ttactttatg
      481 agccacatac gtttcgctac caccatcgtg tcctaccaaa atagcagcca aatgtggagg
      541 tcgttgaccc atgctcttta tagttgctac ttctactgct atttcttgtt taataatatc
      601 cgctattgct tttccgtcaa taagttgcat agttctttta tttgaatttc gggaatttat
      661 taccatttgt ttgtaccata cgcatcattt ttcgtatgcc atcaaattgt tttatcaatt
      721 tgtttacttc ttgtatagaa gtaccactac cattggcaat acgcatttta cgtgtaccat
      781 tcagtaattg aggattttct ctttcggcag gagtcataga ttgtatcatt gcctcaatgc
      841 ctttaaacgc atcatcttta atatcaacat ctttcatggc tttgcccata cctggtatca
      901 ttgctgccaa atcttttagg ttacccatct ttttgatttg ctgaatttga cccataaaat
      961 cattgaaatc aaactgattt ttggcaattt ttttcgctac cctacgagcc tcttcttcgt
     1021 catattgttc ttgggctttt tctactaacg acacaatatc acccatgccc aaaatcctat
     1081 ctgccatacg ttcgggatga aactgatcaa tggcttccat tttttcgccc gttcctacaa
     1141 atttgatagg tttatccact accgaacgaa tagataaagc cgctcctcct cgggtatcac
     1201 catctaattt tgtaagtaca acacccgtaa aatcaagtct atcgttaaat tcttttgcag
     1261 tattcacagc atcttgaccc gtcatcgagt ccaccacaaa caaaatctct tgcggttgaa
     1321 tagcttgttt aatggcagct atttcgttca tcatttgctc atcaatagcc aaacgacccg
     1381 ctgtatcaat aattaacaaa tcgtgtccgt tctttttagc aaaagcaatg gcattttgag
     1441 ctatctgaat gggatttttg ttttctattt cgctataaac aggaacgcct atttgttcgc
     1501 caagcacttt taattgctcg atagcagctg gacgatacac atcacaagca gcaagcaaag
     1561 ggttttttcc tttttttgat ttaagcattt tggctaactt ccccgagaag gttgttttac
     1621 cagaaccttg caaaccagac attaaaatta cagcaggcga acctttggta ttaatatcca
     1681 cggatgttcc acccatcaac aaggctaact catcacgcac aattttaacc atcaactcgt
     1741 tgggcttaat tgctgttagc acattcattc ccaaagcttt ttctttaacc gtatcggtaa
     1801 atgtcttggc tattttatag ttaacatcgg catccaaaag agctttacgc acatctttta
     1861 atgtttctgc tacatttatt tcagtaattt taccctgccc tttaagaatt ttaaacgatc
     1921 gttcgaggcg ttcacttaaa ttttcaaaca taattatctt cggtttagtt taataaatca
     1981 tcaaaaagac agcaaatagt cctttttttg gaaatagctc acaaaattag tcaaaatttg
     2041 tgattttacg cacataaatg tagaataatc atcgaaaatt gatttataaa aaaacagttt
     2101 tttcatctct tttttatgtt ttagtctata attattcact atctttgtgg gtcttaaaat
     2161 aataattaga acggatgaaa aatattcgca atttttgtat tattgcccat attgaccatg
     2221 gtaaaagtac cttagccgat cgtctgctcg aagagactag cacagttaca ggcaaagaga
     2281 tgcaagctca agtacttgat aatatggatt tggaacgaga aagaggcatt accatcaaaa
     2341 gtcatgctat tcagatgaac tacatatatg aaggagaaaa atatgtactc aatctcattg
     2401 acactccagg acacgtagat ttttcgtatg aagtatctcg ttcaattgct gcttgcgaag
     2461 gtgctttgtt aattgtagat gctacgcaag gcattcaggc tcaaacgatc tctaacctat
     2521 acatggctat tgaacatgat ttggaaatta ttcctattat gaataaaatg gacatggata
     2581 gtgccatgcc tgaggaagtt gaagacaaaa ttgtagaatt gctgggatgc aaatcggatg
     2641 aaattataaa agctagcgga aaaaccggtt taggtgtaca agaaatatta caagcaattg
     2701 tcaaacgcat acatccgccg aaaggagacc cagaagctcc tttgcaatgt ttggtatttg
     2761 attctgtatt caattctttc aggggagtta ttacctatat gaaaattgaa aacggaacta
     2821 tacgcaaagg cgatttagta aaattcttta atactggaaa agagtatttt gctgatgaaa
     2881 ttggcgtgct tcgtttaggt atggaaccaa gagaagaatt acgttgtggt gatgttggat
     2941 atattatttc gggcattaag gtatctaaag aagtaaaagt tggcgacact attacacatg
     3001 taaaacgtcc ttgcgaaaaa ggcattgagg ggtttgaaga agtaaagcca atggttttcg
     3061 ctggtatata tcctattgac tcagaggact ttgaagacct aagagcgtca atggagaaat
     3121 tacaattgaa cgatgcctct cttacttttg aagcagagtc atcagccgct ttaggttttg
     3181 gctttcgttg cggatttcta ggcatgttgc acatggagat tgtacaagaa cgacttgatc
     3241 gtgaatttga catggatgta attacgacag tgcccaacgt acagtataaa atatatacgc
     3301 gtaaaaacga agtgctcgac gttcacaatc catcaggaat gccagatata acattgattg
     3361 acagaataga agagccttat atacgagcgt caattattac acgaacggat tttattggtt
     3421 ctatcatgag tttgtgtttg ggaaaaagag gagaattgat aaaacaagat tatatttcat
     3481 ctaatcgtat cgaattaatt tacgatatgc ctatgggaga aatagtcttt gatttttatg
     3541 ataaattgaa gtccatttcc aaaggatatg cttcgtttga ttaccatatc agcggatttc
     3601 gtccttcaaa attggtaaaa atggatattt tattaaatgg agaatcagta gatgccctgt
     3661 catcactcac tcactttgat aatgcggtta caattggtcg tagaatgtgc gagaaattac
     3721 gcgaattaat tcctcgtcag cagtttgata tcgctattca agctgccatt ggttcaaaaa
     3781 ttattgcccg cgaaaccatt aaagctgtac gcaaagatgt tacagcaaaa tgttatgggg
     3841 gcgatatttc acgaaagcgt aaactactgg agaaacagaa aaaaggaaaa aaacgaatga
     3901 agcaaattgg ttcagtagaa gtaccacaaa aagcgtttat ggaggtactt aaattagatt
     3961 agttttcaat attttttaaa taaattattt taccttaaaa ttcaatttct atggaacatg
     4021 tttctgatgt tgcacagcac gttgctatgc caatttgggc atctgcccct tttgcattga
     4081 tgctcattat gattgccatt ggtcctcttt tatgggaaca ctggtgggag caaaacaaaa
     4141 acaaactatt ggtttcattc ttactgagtg taccaacagt tatctttctg attataaatg
     4201 gatttagcca tgatttagaa catcaaataa tgttcgatta tgttcctttt ataattttac
     4261 ttacatcttt gtacgttatt tctggtggct tatttcttac gggagacatt caagccaaac
     4321 caaaaattaa caccttattt atggcaattg ggtacgtgtt agcgtctatt atgggtacaa
     4381 caggagcagc catgctctta attcgcccat taattacaac caatagccaa cgtatgtata
     4441 aaacgcatac cattctattt tttattgctg ccgtagcaaa tgcaggagga ttacttactc
     4501 cactcggaga tcctccatta ttcctattat atctacgtgg agctgatttt ggttggtttt
     4561 tacatatgct tcctgagtgg ttatttatag gtaccgttct aattgggtta tactatttgt
     4621 tagataggag atattataaa aaagaaaaaa cagaagcaat agaaagcgat aatgcaaacg
     4681 tagaaccttt acgtattggg ggtagtaaga atttcttttt gctgattgga gtaattttat
     4741 gtgtagcttt tgtcaatgta aattatattc cagaaatggg caatcctcat ggatctcttt
     4801 ggattaaata tttacgcgaa attattttaa ttgggtttat tttagtatcc atggatatta
     4861 ccaaaaagaa aacacgcgaa ctgaaaggat tctcatggac tcctatttac gaagttgcag
     4921 c
//
LOCUS       DAOPUR010000041         4875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_17459_length_4875_cov_3.088174, whole genome shotgun sequence.
ACCESSION   DAOPUR010000041 DAOPUR010000000
VERSION     DAOPUR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17459_length_4875_cov_3.088174"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /gene="gpmI"
                     /locus_tag="PKN90_01215"
     CDS             complement(<1..1289)
                     /gene="gpmI"
                     /locus_tag="PKN90_01215"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNZ84936.1"
                     /translation="MEKKALLVILDGWGIGDKSKSDIIYNTPTPYWDKLVATYPHSQL
                     QASGESVGLPDGQMGNSEVGHLNIGAGRVVYQDLVKINLACRNNTILDNPAVVNAFSY
                     AKQHNKSVHFLGLVSDGGVHSSLEHLFKLCDIAKTYEIDNTFIHCFMDGRDTDPRSGK
                     GFISELEAHTKKSAGKIASIIGRYYAMDRDKRWERVKESYDLLVNGIGEPQTDMIAAI
                     QKSYDNGVTDEFIKPIVNKAVDGKIKEGDVVIFFNYRNDRAKELTIVLTQKDMPEFGM
                     QTIPNLHYCTMTPYDASFKGMNILFDKDNVNNTLGEYLSGLGKSQLHMAETEKYAHVT
                     FFFNGGREEPFAKEERILINSPKVATYDLQPEMSAYEVKDALVAEIKKQKHDFIVVNY
                     ANGDMVGHTGVYKAIETAVKAVDKCLEETIETAKANG"
     gene            complement(1359..3671)
                     /locus_tag="PKN90_01220"
     CDS             complement(1359..3671)
                     /locus_tag="PKN90_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNZ84937.1"
                     /translation="MKNILIIFLCFLSASTLAQVNETFSDGNFSHAPIWEGNASNFVI
                     NSNAQLQLNAPAEANKSYLVTNSNVTTNAEWKMYGKMMFTPSSANYARWYLIADKQDI
                     TGSLNGYYLLIGNTSKNICLYKQTGTTSTKILDTPINRLNGTTNEINIRITRSSEGLW
                     QLFTQLPTENSPILEGTTTDNTHKAALYTGIVCYYTATRNTGFIFDDIFISGETYEPP
                     ILPSYKASFGDIVFNEIMANPNPPFGLPNEEYIELYNNTSEAINLENYVLKGFSKTCV
                     FPTYVLAPHSYVVVCSTNAFPALEMYGNCLALDKFPTLTNAGGYLELFNAENTILSWV
                     DYSENWHTDSFKKNGGWSLECLDAHNLIGNNTNWNSSINYQGGTPNGANSISGINLDT
                     SELSVINTSIESNSSFVLYFNKPMSVNLLTQTDIITIYPMQEISSIEFLSIKQDAIRI
                     KLTNPLLLHEQYTLNITDVEDINKNKHSIVSRLAIPELPSNQDVVINEVLFNPKSEGT
                     DYVEIVNRSQKVIDLRDIMLTNRKQDGQLAVFPVLSEKGYLLFPGDYCLLSTSKEGVC
                     NYYECPDDIHFVTVPSFPSMPDDKGTIVLTTLSNILIDEFSYSEKMHHTLLSNREGIA
                     LERLHYDITTQDASNWHSASFTCGYGTPGYQNSQFTALIQPTASFQITPKSFSPNNDG
                     NDDTATIYYSFDEAGYFVTIRIYNSNGNCVRNILQNNLSSTEEKTSWDGTDDNGKLLP
                     IGIYIVTIEAFTPTGKRFKSKEVIVLAAKI"
     gene            3922..4875
                     /locus_tag="PKN90_01225"
                     /pseudo
     CDS             3922..4875
                     /locus_tag="PKN90_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843608.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
BASE COUNT         1461 a          823 c          952 g         1639 t
ORIGIN      
        1 tatccgttag cttttgctgt ttcgatagtt tcttctaagc atttatctac agcttttacg
       61 gctgtttcga tagctttgta cacgcccgta tgtcccacca tatcgccatt ggcgtaattc
      121 actacgataa aatcgtgttt ttgttttttt atttcagcaa ctaaagcgtc ttttacttcg
      181 tatgcactca tttcgggttg caagtcgtag gtggctactt tgggcgaatt gattaatata
      241 cgttcttctt ttgcaaaagg ctcttctctt cctccgttga agaagaatgt aacatgggca
      301 tatttttctg tctcagccat gtgcaactgg gattttccta gaccagagag gtattctcct
      361 agggtattgt taacattgtc tttgtcaaac aaaatgttca ttcctttaaa tgaagcatcg
      421 tatggagtca ttgtacaata gtgtaaattt ggtattgttt gcattccaaa ttcaggcatg
      481 tctttttgag ttaacacaat ggtcaattct tttgccctat cattgcggta attaaagaat
      541 attactacat caccttcttt aattttgcca tccacagctt tatttacaat tggtttgata
      601 aattcgtcgg ttacaccatt atcatatgat ttttgtatgg cagcaatcat gtctgtttgt
      661 ggttcgccaa taccgttgac aagcaaatcg tacgattctt ttacacgctc ccaccgtttg
      721 tctctgtcca ttgcataata gcgtccaatg atgcttgcaa ttttaccagc agattttttt
      781 gtgtgagctt ccaattcgct aataaatcct tttccactac gagggtcagt atctctaccg
      841 tccataaagc agtgaataaa tgtattgtca atttcatacg tttttgctat atcgcatagc
      901 ttaaataaat gctccaaaga gctatgtaca cccccatcag acactaagcc taagaagtga
      961 acagatttgt tatgttgttt tgcatacgaa aatgcgttta ccactgctgg gttgtccaaa
     1021 atggtgttgt tgcgacaagc taaattgatt tttactaaat cttgatatac tacacgacca
     1081 gcaccaatgt ttaagtgtcc aacttcagag tttcccatct gaccatctgg taacccaacg
     1141 ctttcgccag aggcttgtag ttgagagtga ggataagttg ctactaattt atcccaatat
     1201 ggtgtaggag tgttatatat aatatctgat tttgatttgt ctcctatgcc ccatccatcg
     1261 aggatgacta acaatgcttt tttttccata taattatttt tttattattt attgaagata
     1321 caaagataca ctattttttt aattgattga agagcccttt agatttttgc cgccaacaca
     1381 atgacttctt tcgatttaaa gcgttttcct gtaggtgtaa acgcttcaat agtaacaata
     1441 tatatcccaa tgggtaaaag ctttccgtta tcatctgttc catcccaaga tgttttttct
     1501 tctgtggatg ataagttatt ttgcagtata tttcttacac aatttccgtt cgagttatat
     1561 atgcgtatag ttacgaaata gccagcttca tcaaaggaat aatagatggt tgctgtgtca
     1621 tcgtttccat cgttattggg agaaaaactt ttgggggtaa tttgaaacga agcggtgggc
     1681 tgtatgagtg ctgtaaattg tgaattctga taacctggtg tgccataccc acaggtaaaa
     1741 gaagctgaat gccagttgct tgcgtcttgt gttgtaatat cataatggag tctttctagg
     1801 gcaatacctt ctctgtttga gagtaaggtg tggtgcattt tttcggaata cgaaaattca
     1861 tcaatcagta tgtttgataa agtagtaagc acgatagttc ctttatcatc aggcatgctt
     1921 ggaaatgagg gtactgttac aaaatgaata tcatcagggc attcataata attacaaacg
     1981 ccttctttgc tagtgcttag taggcaataa tcaccaggaa acaataagta gcctttttcg
     2041 ctcagtactg gaaaaacggc taattgtcca tcttgtttcc tattagtaag catgatgtct
     2101 cgcaaatcaa ttactttttg cgaacgatta acaatttcga cataatccgt cccttcagat
     2161 tttgggttaa ataaaacctc gttgataaca acatcttgat ttgaaggaag ttcaggtatg
     2221 gctaagcgac taacgataga atgtttgttt ttattaatat cttcaacgtc agtaatgttt
     2281 aatgtgtatt gctcatgtaa tagaagtgga tttgtaagtt tgatgcgtat tgcgtcttgt
     2341 tttatggata aaaattcgat agaacttatt tcttgcattg ggtatatagt aatgatatcc
     2401 gtttgtgtga gaagattcac agacattggt ttgttgaaat atagtacaaa agaactgttt
     2461 gattcaatgc ttgtgtttat aacagacaac tcggatgtat ctaagtttat acctgaaata
     2521 ctatttgctc catttggagt tcctccttga taattgatgc ttgagttcca attagtattg
     2581 ttcccaatta aattatgtgc atcgagacat tctaaactcc aacctccatt ttttttgaaa
     2641 gaatcggtat gccaattttc cgaataatcg acccatgaaa gtatggtatt ttctgcattg
     2701 aataattcaa ggtatccacc tgcattggtg agagttggaa atttgtcgag agccaaacaa
     2761 ttaccataca tttctaaagc gggaaaagcg ttggtgctac atactacaac gtagctatga
     2821 ggagccaata catacgtagg gaaaacgcat gtttttgaaa atccttttaa tacatagttt
     2881 tctaaattga tagcttccga agtattgttg tagagttcaa tatattcttc attgggtaat
     2941 ccaaaaggag gatttggatt tgccataatt tcattaaaaa caatgtctcc aaacgatgct
     3001 ttatagctgg gcaaaattgg aggttcatat gtttctccag agataaagat atcatcaaaa
     3061 atgaatcccg tatttcgtgt tgcggtgtaa tagcaaacaa taccagtata tagagcagct
     3121 ttgtgcgtat tgtccgttgt agtaccttct aaaataggcg aattttctgt aggtaattgt
     3181 gtaaataatt gccataaccc ttcgcttgaa cgggtaatgc gaatgttaat ttcgttagta
     3241 gtgccattta atcggttgat gggtgtatcg agtattttgg tggaggtagt acctgtttgc
     3301 ttatacaaac aaatattttt tgacgtatta ccaataagca aataatatcc gttcaatgag
     3361 ccagtaatgt cttgtttgtc agcaattaag taccaacgag cataattagc agaacttggt
     3421 gtaaacatca ttttgccata cattttccat tcggcattgg ttgtaacatt cgaatttgta
     3481 actagatacg atttatttgc ttctgcagga gcatttagtt gaagttgtgc attgctattg
     3541 attacaaaat tggatgcgtt accttcccaa atgggagcat gcgaaaaatt accatcagaa
     3601 aatgtttcat tcacttgagc caaagtactt gccgatagga aacaaagaaa aataatgaga
     3661 atgtttttca tggctataaa aatagagcga caaagtaact attatttttt tgtttgttaa
     3721 aatgttagcc atcaaaaaag cgttgttggc gtcgatgagt gttgcttttt gtgctaaaaa
     3781 gcataaaaaa tactattttt tagtttattt gtttaataat tagaaagtaa agcgtaattt
     3841 tgtagtcagt ttgcgtcgaa aaagaaattt tgactagagg atgcaaatac ttaataagta
     3901 aacgttataa aacagtaaaa aatgaaagta gcaattgttg gtactagcgg tgcagtaggt
     3961 caagagtttt tgcgtgtact cgctgagcgt aatttcccaa tggatgaatt agtattgttt
     4021 ggttcatcac gtagtgcagg atcagaatat gaatttagag gcaaaaagtt ggttgtgcaa
     4081 gaataaaagc atggcgacga ttttaagggt gtagatatcg cctttacatc tgctggtgca
     4141 ggtatttcaa aagaatttgc agccgatatt acaaagtttg gtgctgtaat gatagataat
     4201 tctagtgcat ttcgtatgga tacagacgtg cctttggtag tgccagaagt gaatgctgca
     4261 gatgcaaaaa atagaccacg caatatcatt gcaaatccaa attgtactac tatacaaatg
     4321 gtagttgcat tgcaagcaat agaaaattta tcgcacgtta aacgtgtaca tgtttctact
     4381 taccaagcgg ctagtggagc aggagctgca gctatggatg agttagaatt gcagtataga
     4441 caggtgttaa atggagaaac cccaactgta tccaaatttg cttaccaatt ggcatataac
     4501 cttattccac aaattgatgt atttaccgat aatggatata ccaaagagga gatgaaaatg
     4561 tatcacgaaa cacgcaaaat tatgcactca gatattgaag taagtgccat gtgtgtgcgt
     4621 gtctcctcgt taagagcaca ttcggaagca atttgggcag aaaccgaacg tcctatttcg
     4681 gtagaagaag cacaagctgc ttttgctaaa gctactggtg taattttgca agataatcct
     4741 gcacaaaaag aatatccaat gccactcttt gtagcaggca aagaccctgt atatgtagga
     4801 cgtgtaaaga aagacttagc caacccaaat ggaattttct tttggacagt gagcgaccaa
     4861 attaaaaaag gtgca
//
LOCUS       DAOPUR010000042         4808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_17759_length_4808_cov_3.112981, whole genome shotgun sequence.
ACCESSION   DAOPUR010000042 DAOPUR010000000
VERSION     DAOPUR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17759_length_4808_cov_3.112981"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..730)
                     /locus_tag="PKN90_01230"
                     /pseudo
     CDS             complement(91..730)
                     /locus_tag="PKN90_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467591.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
     gene            complement(742..2859)
                     /locus_tag="PKN90_01235"
     CDS             complement(742..2859)
                     /locus_tag="PKN90_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNZ84938.1"
                     /translation="MILATIFETTLPLTNPVLKFLLILIIILAAPIFLNKIKIPHLLG
                     LIIAGAIIGPFGLNLIERDSGIILSGTAGLLYIMFLAGLEIDIADFKKNTSRSLVFGM
                     YTFIIPMVLGILGSIFILQFSITTSVLLASMFASHTLIAYPLISKLGVVKNKAVTMAV
                     GGTMITDTLALLVLAVIVGMANGEVNSDFWIRLSVSLLAFGLIITILFPIIARWFFKK
                     FDDNVAQYIFVLAMVFLGATLAELAGIEAIIGAFLTGLALNRLIPHTSPLMNRVEFVG
                     NAIFIPFFLIGVGMLVDYRAFFKDWETLKVAAVMTIIATLAKFLAAWLTQKTYKLSLD
                     ERRIIFGLSNAQAAATLAAVLIGYNLQIFDASVLNGTILMILVTCTIASFQAQKGAKN
                     IALSEDLKSSTGEVETDERILIAINQENNLEELVNLGVTIKSKHNKKGLYALHIIDND
                     ESQNNNEKQAKKLLQQAGRIAAATDTKLNELLRYDINLVNGITSSLKEQRITDLILGL
                     NNEKGISDGFFSNLTLGILSKSNPTTLIYKPTQPFGTIKRHLVIVPDKAEKEIGFPFW
                     IIKTWNIARNSGAKIVFYSSIDTQKVIREIQSKHPIECTFKEFSDWDDFLILSRDIEA
                     NDNLIVILSRKDKPSYTPSMQRIPSYLNKYFEGNSYILVYPMQVGVGETSMMDISNPT
                     ILEPIEKLDEIGKTIAKIFRKKT"
     gene            complement(2950..4266)
                     /locus_tag="PKN90_01240"
     CDS             complement(2950..4266)
                     /locus_tag="PKN90_01240"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNZ84939.1"
                     /translation="MQKEGQDIQVFWFRRDLRLTDNVGLYNALQDKRYPVFPLFIFDT
                     DILSSLPSTYDKRVDYIHQILTSLNQELHQLGSSLMVEIGKPTEVFEKLTTQFTIKAV
                     HTNSDYEPYAIQRDKEVRSLLHTKGITLHTYTDQVIFAPGEVIKADGSPYTIFTPFSK
                     IWLRKYEQLPHQEYTTDAYKKNWLSTPAQEIPSLAKCGFKKTSIQYTPPSIPYSIIDN
                     YTDTRNFPAINGTSTLSIHLRFGTISIRQLARIAFTRNHTYVSELIWRDFFMQILYFF
                     PHVQTNAFKPKYDAIEWRNDTAEFSRWCNGTTGYPLVDAGMRQLNETGWMHNRVRMVV
                     ASFLCKHLLIDWRWGEAYFATKLMDYDLSANNGNWQWAAGCGCDAAPYFRVFNPTEQA
                     KKFDSNSEYIRQWIPEVDTNKYATPIVEHTFARNRAIETYKKAISG"
     gene            complement(4269..4799)
                     /locus_tag="PKN90_01245"
     CDS             complement(4269..4799)
                     /locus_tag="PKN90_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNZ84940.1"
                     /translation="MNPWHHVSPHTTQTDSVQAIIEIPKGSRAKYELDKESGLIKLDR
                     VLYSSVYYPVNYGFIPQSYCNDKDPLDILVLSMIELTPMCIVTAKVIGVMQMVDSGAT
                     DNKIIAVAAGDPSVSHYNDIEELPPHFISELRNFFEDYKKLEKKHVEVDEFLNKKAAL
                     EIIAESFVYYDTTFRK"
BASE COUNT         1464 a          873 c          889 g         1582 t
ORIGIN      
        1 tgatttcttt cggaccaacc attcacggag cacattctcc agatgaaaga gccagtatca
       61 aatcatcaca aaaattctgg aaatttgtat tggaaatttt agcaactgag agacaaataa
      121 ggaagctaag gctataaata cagtattgat agtaaataag tagatggcac caaaaaagaa
      181 attcatctga aaagtagcca atccgtaccc agctgtacac aacggaggca taagggctgt
      241 tgcaatggct acgcctggta taacatttcc tttttgtttg ctactaatgg caataatacc
      301 agctaagccc ccaaataagg caatcagtac atcgtatata gatgggcttg tgcgggcaag
      361 taattcagaa tgagctgttg atatggggct taaagtgaac taatatcgta gaggcaacca
      421 aactggcaat tacggcaaaa gaaaagtttt ttactgcacg tttaaataag gcaatatcgt
      481 acgtggcaat gctatacccc atgccattga ttggacccat aagcggagag ataagcatag
      541 caccaatcac cacggctgtt gaattcatat ttaaaccaac agaggctaca atgatagcaa
      601 acatcaaaat ccataaattc gtacccctaa aagcaatatc cttaacaata ctagaatgga
      661 tagcatcgaa ttcatctagt tcggggatga tagtaacatg tgatacaagt ttttttagaa
      721 atatgttcat ttctatgtag gttatgtttt ttttctaaat atttttgcta ttgtttttcc
      781 aatttcatcc aatttttcaa taggttctaa aatggtaggg ttagaaatgt ccatcataga
      841 cgtttctcct acgcccactt gcattggata aaccagtata taactatttc cctcaaaata
      901 tttattgaga tacgagggta tacgttgcat agatggggta taagagggtt tgtctttgcg
      961 actcagaata acaattaagt tgtcatttgc ctcaatatct cttgataata ttaaaaaatc
     1021 atcccaatct gaaaactctt taaaggtgca ttcgattgga tgtttggatt gaatttctcg
     1081 aattactttt tgggtatcta tacttgagta aaagacaatt tttgctcccg aattgcgtgc
     1141 gatattccat gttttgataa tccaaaaagg aaatcctatt tctttttcag ctttatcagg
     1201 tacaattact aaatggcgtt tgatagtgcc aaaaggttgt gttggtttat aaattaaggt
     1261 ggtagggttc gacttgctca atatgcctaa agtaaggttg ctaaaaaatc catctgaaat
     1321 ccctttttca ttgtttaagc ctaaaattaa atcggtgatg cgttgctctt ttaggctact
     1381 tgtgattcca tttactaaat taatatcgta gcgcaatagt tcatttaatt tagtatcagt
     1441 tgcagcagca atacgtcctg cttgttgtaa cagctttttc gcttgttttt cgttattgtt
     1501 ctgtgattcg tcgttgtcaa taatatgtaa agcatacagc ccttttttgt tgtgttttga
     1561 cttaattgtt acccctaaat ttaccaattc ttctaaattg ttttcttggt taatggcaat
     1621 tagaatgcgt tcgtctgttt caacttcacc cgttgaagat tttaaatctt cggataaagc
     1681 aatatttttt gctccttttt gagcttgaaa agatgcaatt gtacaggtta ctaatatcat
     1741 caaaatagtt ccgtttagca cactagcatc gaaaatttga agattgtatc caattaatac
     1801 cgcggctaag gtggctgctg cttgtgcatt gcttaatcca aaaataattc ttcgttcatc
     1861 aagactaagt ttgtatgttt tttgagtaag ccaagctgct aaaaattttg ctaatgtagc
     1921 gataatagtc attactgctg ctacttttaa ggtttcccaa tctttaaaaa atgctcggta
     1981 atcaaccaac attcctacac cgattaagaa gaaaggaata aaaatggcat ttcccacaaa
     2041 ttccactcta ttcatgagcg gagaagtatg tggtatgagt ctatttaaag ctaaccccgt
     2101 taagaatgcc ccaataatag cctctatacc agctaattca gctaaagtag ctcctaaaaa
     2161 gaccattgct aacacaaaaa tatattgggc tacattatca tcaaatttct taaaaaacca
     2221 tcgtgcaatt attggaaata ggatagttat aattaatcca aatgctaaaa gggaaaccga
     2281 taagcgaatc caaaaatcgc tattaacctc gccatttgcc atcccaacta ttactgccaa
     2341 taccaataaa gcaagtgtgt ctgttatcat cgtgcctcct actgccattg ttacagcttt
     2401 attttttact acacccaatt tgctaattaa ggggtaggca ataagggtat gggaagcaaa
     2461 catactggca agaagtaccg atgtggtaat agaaaattga agaatgaata tactgcccaa
     2521 tatgcctaat accataggaa tgataaacgt gtacatcccg aaaacaagac tacgacttgt
     2581 gtttttctta aaatcagcaa tatctatttc taagcctgct aaaaacatga tatatagcaa
     2641 accggctgtc cctgaaagaa tgattccact atcacgttcg attaaattta acccaaaagg
     2701 accaatgatg gctcctgcaa taattaatcc aagcaaatga gggattttta ttttatttaa
     2761 gaatatgggt gcggctaaga tgatgataag aattaacaaa aatttcaaca caggattggt
     2821 taatggtagt gttgtttcaa atatcgtggc taaaatcata ggtggtgtgt tgatttgtgg
     2881 caaagataga aaaaaatatt ctactttata tagctttttt ttaccttttt aagccttttt
     2941 tgtgagaatt taccctgata tagctttttt atacgtttcg atagcccggt tacgtgcaaa
     3001 tgtatgctca acgatgggtg tagcgtactt gtttgtatct acttcaggta tccattgacg
     3061 aatgtattcg ctgtttgaat caaacttttt tgcttgctct gtagggttga atactctaaa
     3121 atagggtgct gcatcgcagc cacaacctgc tgcccattgc cagttgccat tattggcgga
     3181 taaatcataa tccatcaact tggtggcaaa atacgcttcg ccccaacgcc aatctattaa
     3241 taaatgtttg cacaaaaaac tagctactac cattcttact ctgttgtgca tccagcccgt
     3301 ttcgtttagt tgacgcattc ctgcatctac caatggatat ccagtagtcc cattgcacca
     3361 tcgactgaat tctgcggtat cgtttctcca ttcaatagca tcgtattttg gtttaaaagc
     3421 atttgtttgt acgtgtggaa aaaaatacaa gatttgcata aaaaaatctc gccaaatcag
     3481 ttcgcttaca tacgtatgat tacgtgtaaa agctatgcgt gccaattgtc ggatgcttat
     3541 ggttccaaag cgtagatgga tgcttagtgt tgaagtgcca tttatggctg gaaaatttcg
     3601 tgtatctgta tagttgtcaa taatcgagta ggggatactg gggggtgtgt actgtatgct
     3661 cgttttttta aacccacatt ttgcgagtga tggtatttct tgagcaggtg ttgacaacca
     3721 atttttctta tatgcgtcag tggtatattc ttgatgaggt agttgctcgt atttacgtag
     3781 ccatattttt gaaaaaggcg taaaaatagt ataaggactt ccgtctgctt ttattacttc
     3841 gcctggtgca aaaataactt gatcagtata ggtatgtaag gtgattcctt ttgtatgcaa
     3901 caacgaacgt acttctttgt cgcgttgaat ggcgtaaggc tcataatcag agtttgtatg
     3961 tactgcttta atcgtaaatt gagtggtcaa tttttcgaaa acctcggttg gtttgccaat
     4021 ttctaccatc aacgatgagc ctaattgatg caattcttgg ttaagagatg taagaatttg
     4081 atgaatataa tctacccgct tatcatacgt agaaggcagt gaagataaaa tgtctgtatc
     4141 gaaaataaat agagggaata caggatatcg tttgtcttgc aaggcattgt atagaccaac
     4201 attgtcagtc aaacgcaggt cgcgacgaaa ccaaaaaacc tgtatgtctt gtccctcttt
     4261 ttgcatgctt attttctaaa cgtagtatcg taatatacaa aactctcggc aataatctcc
     4321 aaagcagctt ttttgttcaa aaattcatct acctctacgt gtttcttttc cagtttttta
     4381 tagtcttcga aaaagtttct taattccgaa ataaaatgag gtggcagttc ctctatatcg
     4441 ttgtaatgac tcacacttgg gtcgccagct gctactgcta ttattttatt gtcagttgca
     4501 ccgctatcta ccatttgcat tactccaata actttggcag ttactataca catgggggta
     4561 agttcaatca ttgatagcac caaaatatcc aatgggtctt tgtcgttgca ataactttgc
     4621 ggaataaatc cgtagtttac aggataatac actgatgagt acaacactcg atcgagttta
     4681 attaagccac tttctttgtc gagttcgtat tttgccctac ttcctttggg tatttcgata
     4741 attgcttgca cactatccgt ttgcgtagta tgaggcgata cgtggtgcca gggattcatg
     4801 taattagt
//
LOCUS       DAOPUR010000043         4782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_17901_length_4782_cov_3.872223, whole genome shotgun sequence.
ACCESSION   DAOPUR010000043 DAOPUR010000000
VERSION     DAOPUR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17901_length_4782_cov_3.872223"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="PKN90_01250"
     CDS             <1..1634
                     /locus_tag="PKN90_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ84941.1"
                     /translation="KQQTKRFANYASQKVHRVVPFVNNHDTFRPILDDDGKYKGWDYS
                     NQIGGNVDPFDKRLGMAYAIIMGVDGNPGIFMEDLFNLSNGKRFSHDPKNETDLPVRS
                     SVRNLIWCHQNLGFKYGDYKIRYQSGDLLVIERAGKAIIAITDNGSTDQEAWIDTDFR
                     NVDMKDYSGSISGTRRAWGDGRFNVKVTNTGTGLGYAVWAPDGITGGYEPYRSKSTTQ
                     EWEMADDLGDSNCQSLGQGGAIPASTHQRVVGKIYVEENKLVTYNFFPQQDGYDITLA
                     LYDLEGNLLKKQSDNKGFTGEWNATYTGWVTLKVWNNNSENISQKCWVRVKYYAPPTI
                     TDVYTDTPDTRVSIWTANGNTSTWNDCNNWEQGKVPANNATVIIPNHTPIVPIMSGNL
                     TLKKLVIEQGKGNIGSPDLIVNGSLTVNELTILSGTAYICGNGTTEVSIQNGAVSNCT
                     TRIEEPSFSIEHISVIPNPVESICELRFISNSIGVAYFTLTDVTGKTILTKEIPINAI
                     GINSTNLYLGGIHTGTYIAKINCNNRISSVMIVKK"
     gene            1732..2187
                     /locus_tag="PKN90_01255"
     CDS             1732..2187
                     /locus_tag="PKN90_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84942.1"
                     /translation="MKKTILIVALLVGFVASGVAQSRSIGLRSFQEVSYQHGFGSSNM
                     LEIDGGFFNTGLQATATYNWLFPIDWDYEGSWNWYVGPGAYAGIRSNSNNNDGLFIGA
                     AGMIGIEYNFWFPLQLSIDYRPTIALFNSSNMPFGFYSNTVGLGVRYIF"
     gene            complement(2272..3210)
                     /locus_tag="PKN90_01260"
     CDS             complement(2272..3210)
                     /locus_tag="PKN90_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84943.1"
                     /translation="MEPEKELDLIDLLKLLGNSIGKGISAISRFIMWFIKFCVKNWYI
                     LLVSVAFGIACAFYFSQSKFSKYEGIIVVENNVESTPEFYTEVQSLASYMNNDNTSFS
                     SKFGVSTEIGKKIFKLEPLYIYTSENEGLYNFIDEKKKYSDLEPHQQKFAILIKTNDR
                     SLFPFLKTALYKYFENHPYFKGRNVARLNSLDVQQRTLQNEIATLDSLKKLEYFEANS
                     KTKNLKMDNTLLIGESNTQLYHTNILEMMEKLQRTKIDIETRNSVVTISSDFQIKSPQ
                     NARVKSLVLFGSAFFVIGFCLALFIRHRNKIKEFLK"
     gene            complement(3343..3951)
                     /locus_tag="PKN90_01265"
     CDS             complement(3343..3951)
                     /locus_tag="PKN90_01265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ84944.1"
                     /translation="MPIFKENMDIHKRLLYHAFVFPFLFLFIFWLVFLVERVLYTDFT
                     VFGIFPLATKGLLGIITMPFIHADSTHLLANSVTFLVLSVLLFSNYRHIALKVFMLIW
                     LFSGVILWVIGRPSWHIGASGVIYGMAFFLFCSGLLRKKIPLVATSLVVVFLYGGMVW
                     YMFPLGIDNHISWEGHLSGALIGMFFSVYYKIKEPSEQKQGI"
     gene            complement(3952..>4782)
                     /locus_tag="PKN90_01270"
     CDS             complement(3952..>4782)
                     /locus_tag="PKN90_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNZ84945.1"
                     /translation="IENMQKHGIPASITLAQGILESGIGDSRLAREANNHFGIKCHDW
                     KGEYILHTDDKVNECFRKYKNPEESYEDHSVFLTTRGRYAHLFELKITDYKAWAFGLQ
                     RAGYATDPTYATRLINLIEENGLHTFDKQLPQPPKQKSVAKEETKHDTEKPSREHKHD
                     RYPSRPTQRNMYGLRYVIASSNDTYTSIAKEFKVSLRSILKWNDLPYEVSLKEGEIVY
                     LQSKKKRVSSYSTVHYVASGETMHIISQRYGIRLKSLYDLNNMNYSQVPTIGQKLYLR
                     "
BASE COUNT         1666 a          827 c          781 g         1508 t
ORIGIN      
        1 ggaaacaaca aacaaaacgc tttgccaact acgcttctca aaaagtacat agagtagtac
       61 ctttcgttaa taaccatgac accttcagac caatattaga tgatgatggg aaatataaag
      121 gctgggatta cagcaatcaa ataggaggaa atgtagatcc ttttgacaaa cgtttgggaa
      181 tggcatacgc cataataatg ggagttgatg gcaatccagg catatttatg gaagatcttt
      241 ttaatctaag caacgggaag agatttagtc atgacccaaa aaatgaaaca gacctgcctg
      301 tgcgaagttc tgttagaaac cttatttggt gtcatcaaaa tcttggattt aaatatggcg
      361 actacaaaat acgctatcaa tcaggcgatt tactagtaat tgaacgtgca ggaaaagcaa
      421 taattgctat tactgataat ggctctaccg accaagaagc atggatagat acagactttc
      481 gtaatgtaga tatgaaagat tactctggta gcattagcgg tacacgaaga gcatggggag
      541 acggacgttt taatgtaaaa gtaacgaata caggaacagg attaggttat gccgtatggg
      601 ctccagatgg gataactggc ggatacgaac cataccgatc caaatcaaca acacaagaat
      661 gggaaatggc tgatgactta ggcgatagta attgccaatc gttaggacaa ggaggagcca
      721 ttcctgcatc aacgcatcaa cgtgtagttg gcaaaatata tgtagaagaa aacaagcttg
      781 taacctacaa ctttttccca caacaagatg gatatgatat aacattggca ttgtatgact
      841 tagaaggaaa tctcttaaag aaacaaagtg ataacaaagg atttactgga gaatggaatg
      901 caacttatac agggtgggta acactgaaag tgtggaataa taattctgaa aatatctcac
      961 aaaaatgttg ggtaagagtg aaatactatg ctccgcccac tattacggat gtgtatacag
     1021 atacccctga tacacgagta agtatttgga cagcaaacgg taatactagc acatggaacg
     1081 attgtaataa ttgggaacaa ggcaaagttc cagctaataa tgctacggtt atcattccta
     1141 atcacacccc aattgtgccc attatgtctg gaaatcttac attaaaaaaa ttggttattg
     1201 agcaaggtaa aggcaatatt ggaagtcctg atttgatagt taatggttct ttaacagtaa
     1261 acgaattgac tattttatct ggtacagcgt atatttgtgg caatggtaca acagaggtat
     1321 ctattcaaaa tggtgctgta tcaaattgta caacacgtat tgaagaacct tcttttagta
     1381 ttgaacacat atcagtcata cccaatccag tagaatctat ctgcgaattg cgttttattt
     1441 ccaatagtat cggtgtagct tatttcacat taactgatgt aactggaaaa acaattctga
     1501 caaaagaaat ccctattaat gctattggga tcaattctac caacttatat ttaggaggta
     1561 tacatactgg aacatatatt gcaaaaataa actgtaacaa ccgaatttct tctgtaatga
     1621 ttgttaaaaa ataaggaata acaactaatt ttaattgctt tttcagaaaa taaaccttta
     1681 ctttgcacgc gaaaaagtaa aggtttattt tttaactaaa acgaataaat tatgaaaaaa
     1741 acgattttaa ttgtagcctt attagtaggc tttgtagcaa gtggtgtagc acaatcacgt
     1801 tcaataggat tacgatcttt ccaagaagta agttatcaac atggatttgg tagtagcaat
     1861 atgctagaaa ttgacggagg attctttaat acaggtttac aagcaaccgc tacatacaat
     1921 tggttatttc caattgattg ggattatgaa ggcagttgga attggtatgt aggtccaggt
     1981 gcatatgcag gtattcgctc gaacagtaat aacaatgatg gattatttat tggagcagca
     2041 ggtatgattg gtatcgaata taacttttgg tttcctcttc aattatcaat agattaccgc
     2101 ccaacaattg ctttatttaa tagtagtaat atgccttttg gattctactc aaatactgtt
     2161 ggattaggag ttaggtatat tttctaacaa aattacaagt gtatagtctt tataaaacaa
     2221 tagcgagttc aaaaagttga actcgctatt gttttataac tgatgaacgt gttattttaa
     2281 aaactccttg attttgttcc tatggcgaat aaatagagcg agacaaaaac caataacaaa
     2341 gaaagcagaa ccaaataaaa ctaaagactt tactctagca ttttggggag attttatctg
     2401 aaaatcagaa ctaatcgtta ctacgctatt tcttgtttca atatcaattt tcgtacgctg
     2461 caatttttcc atcatttcta aaatattagt atgatacaac tgcgtattgg actctcctat
     2521 taataaagta ttatccattt ttaaattttt tgttttagaa ttagcctcga aatattccaa
     2581 ttttttcaaa ctatccaacg tcgctatttc attttgcaat gtgcgttgtt gcacatctaa
     2641 agaatttaac cgagccacat tccgcccttt aaaataggga tgattctcaa aatatttgta
     2701 caatgctgtc tttaagaacg ggaataacga acgatcattt gtctttatta aaatagcaaa
     2761 tttttgttga tgaggctcca aatctgaata cttctttttt tcgtcaataa aattatacaa
     2821 tccttcattt tcgctggtat aaatataaag aggttccaac ttaaatatct ttttcccaat
     2881 ttcagttgaa acaccaaatt tagacgagaa agatgtatta tcattgttca tataagatgc
     2941 caaagactgt acttctgtat aaaactcagg ggtactttct acattattct ctactacaat
     3001 tattccttca tattttgaaa atttagattg tgaaaaataa aaagcacaag ctattccaaa
     3061 agctacggaa acaagtagta tataccaatt ttttacacaa aacttgataa accacataat
     3121 aaatctacta atagcagata ttcctttacc tatcgaatta cctaacaatt ttaataaatc
     3181 aattaaatct aattcctttt ctggttccat actgtttctg ttgtttatat ttgtaactct
     3241 tttgatgcac aaaaatacat aaaatattga tttaaatagt atcttctgtt gtattttcta
     3301 tctcgttctc ttgccaatca taatcaacac tatcattttc atctatatcc cttgtttttg
     3361 ttcagatggc tctttgattt tataataaac agaaaaaaac atacctatca acgctcctga
     3421 caaatgccct tcccacgaaa tatgattatc aatacctaaa ggaaacatat accacaccat
     3481 acctccatat agaaaaacta caaccaaaga agtagcaact aagggtattt tttttcgaag
     3541 caatccacta caaaaaagaa aaaaagccat tccataaatt actccacttg cccctatatg
     3601 ccaacttggc ctaccaataa cccataatat tacgcctgaa aaaagccaga taagcataaa
     3661 tacttttagt gcaatatgcc tataattaga aaacaacaaa acagataaga ccaaaaaagt
     3721 gacggaatta gctaggagat gtgtgctatc tgcatgaata aatggcattg ttataattcc
     3781 caataaccct ttagttgcta gaggaaatat gccaaataca gtaaaatcag tatagagtac
     3841 cctctctacc aaaaatacaa gccaaaaaat aaaaagaaat aaaaaaggaa aaacaaacgc
     3901 atgataaagt aaacgtttat gaatatccat attttcttta aaaataggca tctatcttag
     3961 gtataatttt tgaccaatag ttggtacctg agaataattc atattattca aatcatataa
     4021 cgattttaat cgtattccat aacgttgaga aattatatgc atcgtttctc ctgaggcaac
     4081 ataatgtact gtactataac tcgacacgcg ttttttcttg ctttgcaaat aaacaatttc
     4141 tccttctttc aaacttacct catagggcaa atcattccat ttgagtatag aacgtaaaga
     4201 taccttaaat tccttagcta tagatgtata cgtatcatta gaagaggcaa tcacataacg
     4261 taatccgtac atattccgtt gtgttggtcg tgatggatat ctatcatgct tgtgttctcg
     4321 tgatggcttt tcagtgtcgt gtttcgtttc ttccttagca actgattttt gcttaggagg
     4381 ctgtggaagc tgtttatcaa atgtatgcaa tccattttct tcaattaagt tgattaatct
     4441 ggtggcataa gttgggtctg ttgcataacc tgcacgttgc aaaccaaatg cccaagcctt
     4501 ataatcggta attttcaact caaataaatg tgcatagcga cctcttgtgg ttaaaaatac
     4561 cgaatgatct tcgtacgatt cttcaggatt tttatatttt ctaaaacatt catttacttt
     4621 atcatcagta tgtaaaatat attccccttt ccaatcatga catttgatac caaaatgatt
     4681 gttggcttca cgagccaaac gactatcccc gatacctgac tccaaaatcc cttgagccaa
     4741 tgtaatactt gcaggaattc cgtgtttctg catattttct at
//
LOCUS       DAOPUR010000044         4755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_18056_length_4755_cov_3.158936, whole genome shotgun sequence.
ACCESSION   DAOPUR010000044 DAOPUR010000000
VERSION     DAOPUR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18056_length_4755_cov_3.158936"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKN90_01275"
     CDS             <1..602
                     /locus_tag="PKN90_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNZ84946.1"
                     /translation="ANKIANPGCFATVIELALLPLAANGLLTNEVHINAITGSTGAGV
                     KPSSTSHFSWRNNNISVYKAFEHQHLKEITQSITQLQPSFGKAINFIPVRGNFARGIF
                     ASVYTDCSLSLEEAKALYKNYYKDAAFTFVTDTNPDLKEVVNTNKCIVYLEKHGDKLL
                     ILSCGDNLLKGASGQAVQNMNLLFGLDEKMGLNLKNIAF"
     gene            643..1764
                     /locus_tag="PKN90_01280"
     CDS             643..1764
                     /locus_tag="PKN90_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ84947.1"
                     /translation="MKLFDVYPLFPIEIVKGKGCKVYDNNGTEYLDLYGGHAVISVGH
                     AHPTYVQAVADQVGKLGFYSNSVINGLQETFAEKLGIACGYPDYSLFLINSGAEATEN
                     ALKLASFHNGKKKVLAFKKAFHGRTSVAVKVTDSPSYVAPINAGLDVTFLALNDIEGV
                     KKELATGDYTAVIIEGIQGIGGIIEPNIDFLQALRLECTQTNTVLILDEIQSGYGRSG
                     KFFAHQYAGIKADIITVAKGIANGFPFAGVLISPMFKAVHGQLGTTFGGNHLGCAAAL
                     AVLNIIEKENLIENAKQIGDYLLHELKQIPQIKEVRGKGLMIGIDFDQAVKEIRNKLL
                     VEQHVFTGVAGTHTIRLLPPLCLTKEEATEFLLKLKQCL"
     gene            1814..2335
                     /locus_tag="PKN90_01285"
     CDS             1814..2335
                     /locus_tag="PKN90_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNZ84948.1"
                     /translation="MISKKMQDAINAQINAEFWSAYLYLSMSNNFAAKGNPGFANWFE
                     IQFKEEQDHAMIFGKHILARGGKVSLAPIKEVKTEWATPLEAFEETLKHEKVVTGLIN
                     NLYKIAVEENDYATQSMLKWFIDEQVEEEETAQELIDSLKMIANNGFGIYMLDKELST
                     RTYKTPSPLVAKA"
     gene            2427..3230
                     /gene="proC"
                     /locus_tag="PKN90_01290"
     CDS             2427..3230
                     /gene="proC"
                     /locus_tag="PKN90_01290"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNZ84949.1"
                     /translation="MKKISIIGAGNMGGAIARGLSQGSIFSAQQITVSDISTENLQAI
                     KTFNPLINTTLSNVDGVQHADIVVIAVKPWLTETICNEIKSVLNYDKQVVVSIVAGVS
                     FEQLHTFLKKEGESSPTIFRVIPNTAIDVLQSVSTIASSHANKQQEEEIVRIFNELGK
                     AFLVSSEQQLNAFMSLSSCGIAYAFRYIRAAVEGGVEMGIYPETAKQVVIQTLRGAID
                     LLEATNAHPEAEIDKVTTPAGITIKGLNEMEANGFSNAVIKGLKASYIK"
     gene            complement(3236..>4755)
                     /gene="nadB"
                     /locus_tag="PKN90_01295"
     CDS             complement(3236..>4755)
                     /gene="nadB"
                     /locus_tag="PKN90_01295"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNZ84950.1"
                     /translation="FALKVAHKGKVAILSKTRLEEANTNLAQGGIASVTKDSDNFEKH
                     IQDTLICGDGLCDEEVVRMVVENAPSQIDELVKWGVDFDKGDEGNFDLHKEGGHSDFR
                     ILHHKDATGAEIQKRLVRQIKKHPHIDVFENHFAVEIITQHHLGMLVKHTTPNIECYG
                     AYMLNEKTRHVHTFLAKVTMMATGGIGSIYQSTTNPSIATGDGIAMVYRAKGLVSDME
                     FVQFHPTSFYNPNERPSFLITEAMRGYGAVLRTRKGRQFMQKYDERGSLAPRDIVARA
                     IDSEMKLSGDDFVYLDVTHKSAEETKAHFPTIYQKCLSLGVDITKDYIPVVPAAHYLC
                     GGIEVDVYSQSSIRNLYAAGECSRTGLHGANRLASNSLIEAVVYADKAATHALGRIEG
                     IVFNEQVPEWNDEGTSLNEEMVLITQSAKEVGQIMSNYVGIVRSNLRLKRAMDRLEIL
                     YKETEDLFTRSVVTKDICELRNIINTAYLVIKMAMQRKESRGLHYTLDYPDKIEL"
BASE COUNT         1548 a          910 c          880 g         1417 t
ORIGIN      
        1 aagcgaataa aatcgctaat ccaggatgct ttgcaacggt cattgaattg gctctgttac
       61 ctttggcagc taacggattg cttaccaacg aagtgcatat aaatgctatt acaggctcta
      121 caggggcagg agtaaaacca agtagcacat cgcattttag ttggagaaac aataatattt
      181 ctgtttacaa agcatttgaa caccaacacc tgaaagaaat tacgcaatct attacacaat
      241 tgcaaccctc ttttggcaaa gcaattaatt ttattcctgt aaggggtaat tttgctcgtg
      301 gtatttttgc ttctgtatac accgattgca gcctatcgtt agaagaagcc aaagccttgt
      361 acaaaaacta ttacaaagat gctgcgttta cgtttgttac agatacaaat cctgatttaa
      421 aagaagtagt aaacacaaac aagtgtatcg tatatcttga aaaacatggc gataaattac
      481 ttattttatc gtgtggtgat aatttattga aaggggcttc tggacaagca gttcaaaaca
      541 tgaatctact atttgggctt gacgaaaaaa tgggcttaaa tttgaagaat attgcatttt
      601 agttacaaaa ctacattgtt cgttatctta aaaaatgaaa aaatgaaatt atttgatgta
      661 tatccgctat tccctattga aattgtaaaa gggaaaggat gcaaagtata tgataacaat
      721 ggtactgaat acctcgactt atatggtgga catgctgtta tttctgttgg gcatgcacac
      781 cctacgtatg tgcaagcagt agcagatcaa gtaggaaaat tagggtttta ctccaattcg
      841 gtaatcaatg gcttgcaaga aacatttgcc gagaaattag gcattgcgtg tggataccct
      901 gattattcgc tattcttaat caactcgggt gcagaagcta ctgaaaatgc actgaaatta
      961 gcttcctttc ataatggaaa aaagaaagta ttagcattca agaaagcctt tcatggacgt
     1021 acctctgtag ctgtaaaagt aacggatagc ccaagctacg tggcacctat caacgctggt
     1081 ttagacgtaa catttttagc tcttaacgac attgaaggtg taaaaaaaga acttgcaaca
     1141 ggcgattata cggctgttat catcgaaggc atacaaggta ttggcggtat tattgagccc
     1201 aatatcgatt ttttgcaagc attacgtttg gaatgtacac aaacaaatac ggtacttatt
     1261 ttggatgaaa ttcaatcagg atacggtcgt agtggcaagt tttttgctca tcaatatgct
     1321 ggcatcaagg cagatatcat tacggttgcc aaaggtattg caaatggatt tccttttgct
     1381 ggtgtgctta ttagtccaat gtttaaagcc gttcacggac aattaggcac aacttttgga
     1441 ggcaatcatt taggttgtgc cgctgcatta gctgtattga acattatcga aaaagagaat
     1501 cttatcgaaa atgcaaaaca aattggcgat tacctactac acgaactaaa acaaatccct
     1561 caaataaaag aagttagagg caaaggattg atgattggta tagattttga tcaagctgta
     1621 aaggaaattc gaaacaaatt attagtagaa cagcacgtat ttacaggcgt agcgggcaca
     1681 catactatcc gtttattgcc tccactttgt ttaacaaaag aggaagctac tgaatttttg
     1741 ttaaaactaa aacaatgttt gtaaaaatgt tgtatatttg tggtaatgta taacccataa
     1801 aaaatgaacc gttatgatta gtaaaaaaat gcaagatgcg attaacgcac aaataaatgc
     1861 agaattttgg tctgcatacc tttatttatc tatgtccaat aattttgctg caaaaggaaa
     1921 tcctggtttt gcaaactggt tcgaaataca gtttaaagaa gaacaagacc atgcaatgat
     1981 ttttggcaaa catattttgg cacgtggagg aaaagtatct ttggctccaa tcaaagaagt
     2041 gaaaaccgaa tgggcaactc cgttggaagc ttttgaagaa acccttaaac acgaaaaagt
     2101 ggttacaggt ttaatcaata atctatacaa aattgctgta gaagaaaatg attacgctac
     2161 ccaaagtatg ctaaaatggt ttatcgacga gcaagtagaa gaagaagaaa cagctcaaga
     2221 gctaatcgat agcctaaaaa tgattgccaa caatggtttt ggtatttata tgcttgataa
     2281 agaattaagc actcgcacat acaaaacacc aagtccttta gtagcaaaag cataaaagaa
     2341 aattcgtaag gtcgaaaatg gagtattctt tttcgacttt atttttaact atcatttact
     2401 ggattggaat taataacata ctttttatga aaaaaatcag cattattgga gcaggcaata
     2461 tgggtggagc tattgctaga ggattatctc aaggttctat cttttcagcc caacagatta
     2521 ctgtaagcga tatttcaacc gaaaacttac aggcaattaa aaccttcaat cctcttatta
     2581 atacgactct tagtaatgta gatggggtgc aacatgctga tattgttgtg atagctgtaa
     2641 agccttggct taccgaaaca atttgtaacg aaattaaatc tgtattaaac tatgacaaac
     2701 aagttgttgt gtcaattgtt gctggtgttt cttttgagca gttacataca tttttaaaga
     2761 aagaaggaga gtccagtcca accatattta gggttattcc aaatacggct attgatgtat
     2821 tgcaaagcgt ttcaaccata gcctcatcgc atgcaaacaa acaacaagaa gaagaaattg
     2881 tacgtatatt caatgaactt gggaaagctt ttttagttag ttccgaacaa caactcaacg
     2941 catttatgtc gctttcaagt tgtggtattg cgtatgcctt tcggtatata cgtgccgctg
     3001 tagaaggtgg agtagaaatg ggcatttatc cagaaacagc caaacaagta gtgatacaaa
     3061 cattgcgagg agcaattgac ctactcgaag ctaccaatgc acatcccgaa gccgaaattg
     3121 acaaagtaac cacacctgct ggtattacca ttaaaggctt aaacgaaatg gaagccaacg
     3181 gattctcaaa cgctgttatc aaaggcttaa aagcatcgta tataaaataa cccgattata
     3241 attcaatctt atcggggtaa tcgagtgtgt aatgcaaccc cctactttct ttgcgttgca
     3301 ttgccatttt aattactaaa taggcggtat tgatgatatt acgaagttcg caaatatctt
     3361 tggttaccac tgaacgggta aacaaatctt cggtttcttt gtacaaaatt tctaatcgat
     3421 ccatggctcg tttcaaacgc aaattagacc gtacaatacc cacataatta gacataattt
     3481 gccccacctc tttggcactt tgtgttatca acaccatttc ttcgttcagc gaggtacctt
     3541 catcattcca ttctggcact tgctcattaa aaacaatacc ttcaatcctg cctaacgcat
     3601 gcgtagcagc tttgtcggca tacactaccg cttcaattaa agaattagat gccaaacggt
     3661 tagcaccatg caatccagtt cgcgaacatt ctccggctgc gtacaaattg cgaatacttg
     3721 actgcgaata tacatctacc tctataccac cacacaaata atgagctgcg ggcactacgg
     3781 gtatataatc tttggtaata tccacaccga gcgacaaaca tttttgataa attgtaggga
     3841 aatgtgcttt tgtctcctct gccgatttat gtgtaacatc taaatagaca aaatcatctc
     3901 ccgaaagttt catttcagag tcgatagccc gtgccactat atcacgtgga gctagcgaac
     3961 cacgttcatc gtatttctgc ataaactgac gccctttgcg tgttcgcaat acagctccgt
     4021 atcctcgcat tgcctctgtt attaaaaaag agggtctttc atttggatta taaaaagacg
     4081 ttggatgaaa ctgtacaaat tccatatcgc tcaccagtcc ttttgctcta tacaccatgg
     4141 ctataccatc gcctgtagca atagatggat tggtcgttga ctgataaata ctgcctatac
     4201 ctccagtagc catcattgta acttttgcga gaaaagtatg tacatgacgc gttttttcgt
     4261 tcagcatata cgctccataa cattcgatat tcggtgtagt gtgcttcacc aacatgccca
     4321 aatggtgttg ggtaataatc tcaacagcaa aatgattttc aaaaacatca atgtgtgggt
     4381 gcttttttat ttgtcgcacc aatcgtttct gaatttcagc tcccgtagca tctttgtggt
     4441 gcaaaatacg aaaatcagaa tgacctcctt ctttgtgcaa atcaaaattt ccctcatctc
     4501 ctttatcaaa atcaacaccc cattttacga gctcatctat ttgcgaaggt gcattctcaa
     4561 ctaccatacg caccacctct tcgtcgcata atccatcgcc acaaatcaat gtatcttgaa
     4621 tatgtttctc aaaattatcc gaatctttgg taacagaggc aatgcctcct tgagctaaat
     4681 tggtattggc ttcttctaat cgtgttttac tcaaaatagc cactttccct ttatgggcaa
     4741 ccttaagggc aaaac
//
LOCUS       DAOPUR010000045         4635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_18675_length_4635_cov_2.947598, whole genome shotgun sequence.
ACCESSION   DAOPUR010000045 DAOPUR010000000
VERSION     DAOPUR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18675_length_4635_cov_2.947598"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PKN90_01300"
     CDS             <1..1300
                     /locus_tag="PKN90_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ84951.1"
                     /translation="ALWITPFFDNNDKNYSYHHYAAGDYYKVDPRLGTKENYKELSTK
                     CKENGLKLIIDVVPNHCGSAHWWMKDLPASDWLNKWDTYTSSNYRMTAWTDPYSSEYD
                     VKQLNKGWFSENMPDYNLANPHLFTYLKQAYIWWIEYADVDGIRVDTYPYTDIRIGSK
                     WIKAIRDEYPTINIVGECWVKTPQEIAFYQSGSMNKYGFDSHMQSVMDFVLKDVFEFS
                     FNERESWDRGMIRFYNHYAQDFLYADPNQIMNFLDNHDIDRYSEVVGHDVKKYKMALA
                     LLIASRGYPQIYAGNEIMLGGNRGSYEGHRFDFPGGFPGDKRNAFTPEGRTEIENDVF
                     NYLRTLLHYRKNTTALQTGKKKQFIPYDGIYVYFRYDDNATVMVIANNNDTSRKVKTD
                     RFVEVLAPFTKGKDVTSGKNFDITNSVEIPGKTVLLLELE"
     gene            1326..2669
                     /locus_tag="PKN90_01305"
     CDS             1326..2669
                     /locus_tag="PKN90_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ84952.1"
                     /translation="MEKPRLRFWQIWNMSFGFLGIQFGFALQNANVSRIFETLGADIG
                     NIPILWIAAPVTGLIIQPIIGHMSDKTWNKLGRRRPFFLAGAILASLALIFMPNSPYL
                     WVAAGMLWIMDASINISMEPFRAFVGDMLPAEQRTKGFAMQSFFIGTGAVVASALPYM
                     LTNWFGIANTAPEGLIPPSVKYSFYLGAIAFLVTVIWTVISTKEYSPEELATFEKNEQ
                     KENESTSNHHEAILPSSRFLKIAPIWTITGILLSVLVYFFSLEAELYILTGGITFFGL
                     ILFFVGGLVQANKQKNGLVEVINDLLLMPKTMKQLAIVQFFSWFALFAMWIYTTAAVT
                     SHIYGTSDTHSAIYNEGADWVGIMFAAYNGFAAIVAFLLPVLANYTNRRFTHVIALIA
                     GAVGLISIYFVENPTMLLLPMLGVRFGLVKYFIYALCYLNRVIAIAQNGNIHGNI"
     gene            2656..2838
                     /locus_tag="PKN90_01310"
     CDS             2656..2838
                     /locus_tag="PKN90_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84953.1"
                     /translation="MGIFNFFIVIPQIMAATILGFFVTKIAGNHAIYALLLGGVSFLI
                     AAVMTLFVKDEDEKNM"
     gene            2870..>4635
                     /locus_tag="PKN90_01315"
     CDS             2870..>4635
                     /locus_tag="PKN90_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ84954.1"
                     /translation="MKKLLLLVGIIALLVACSARGMLKLPVKSENNMRLNSPLVLVKG
                     SNFFLTQDYVIDITTIDSITADAKNGISVSLSANKDSAYINATDMPQLVNISLWSKGV
                     PYGVLAKKTNKIDYVFTYDATGDKGTRYQIAGQMNDWFPAGHPDLVKNEKGLYEVTLN
                     LSPGAYLYQMVIDGDWNHDKNNPDKVDNNYGKFNSILHVKGTQEKAPKLLTQSFDENN
                     IVLSVDNAAEQVYVYWQNYLLPNDLFVKNKGQQLIVNLPKEAIETDRSFLRIFTANEY
                     GISNDVLIPLQKGKVLNNVADITRFDKHAEAIYFLLVDRFKDGNEANNFPMNRPDVNP
                     KVDYQGGDIAGITQKIKDGYFQRLGFTTIWVSPIVQNPMEPWGYWANPETKFSGYHGY
                     WPISSSRIDFRFGTDEEMHELVKTAHENNINIIVDYVANHVHKNHPLYQRDSTIATPL
                     YLPDGTKNTERWNEYRLTTWFDEFMPSIDFSRQENIDMMVDSALYWVKKFDLDGFRHD
                     ACKHVNEEFWRTLTHRMKTEVEIPRGKLTYQIGESYGSPQLISSYVNTGMLDAQFDFN
                     VFDDAASVFAFNNEDFVRVSNG"
BASE COUNT         1533 a          793 c          910 g         1399 t
ORIGIN      
        1 agccctatgg ataactccat ttttcgataa taacgataaa aactattctt accatcacta
       61 tgccgcagga gactactaca aagtggatcc tcgtttaggc acaaaggaaa attacaaaga
      121 actttcgaca aagtgtaaag aaaatggatt gaagctcatt atagatgtgg ttcctaacca
      181 ttgtggaagt gcacattggt ggatgaaaga ccttcctgca tctgattggc taaataaatg
      241 ggacacatac acttcttcta attatcgtat gactgcatgg acagacccgt attcgtctga
      301 atatgatgta aaacaattaa acaaaggatg gttttcagaa aatatgccag attataatct
      361 tgcaaatcct catctgttta cctatctaaa acaagcctat atttggtgga tagaatatgc
      421 cgatgtggat ggcatacgtg ttgacacgta tccctataca gacatccgta taggatcaaa
      481 atggattaaa gctattcgcg acgaatatcc gactatcaac atagtaggtg aatgttgggt
      541 aaaaacgcct caagaaattg cgttctatca atcagggtca atgaataaat atggttttga
      601 ctcgcacatg caaagtgtaa tggacttcgt actgaaagat gtatttgaat tttcatttaa
      661 cgaacgtgaa agttgggaca gaggcatgat tcgtttttac aatcattacg cacaagactt
      721 tttgtatgct gaccccaatc aaattatgaa tttccttgat aaccacgata ttgatcgtta
      781 ttcggaagta gtgggtcacg atgtgaaaaa atacaaaatg gcattagcac tgttaatcgc
      841 gtcgcgagga tacccacaaa tatatgcagg taacgaaatt atgctaggag gtaacagagg
      901 ctcatacgaa ggtcatcgtt ttgattttcc tggtggtttt ccaggcgata aacgcaatgc
      961 ttttacaccc gaagggcgta cagaaataga aaacgatgtg tttaattatt tacgaacctt
     1021 gttgcattac cgaaaaaaca ccacagcatt gcaaacagga aagaaaaaac aatttatccc
     1081 atacgatggc atttatgtat attttaggta tgatgacaat gcaacagtaa tggtaatagc
     1141 taataataat gacactagcc gaaaagtaaa aacggaccgt tttgtagaag tattagcccc
     1201 tttcacaaag ggtaaagatg ttacctcagg taaaaacttt gatataacaa atagcgtaga
     1261 gataccaggt aaaacagtgt tattattaga attagaatag caaattaaaa aacacagcat
     1321 tcaaaatgga aaaacctcgt ttacgttttt ggcaaatttg gaacatgagt tttggctttt
     1381 tgggcataca gttcggattt gctttacaaa atgccaatgt aagtagaata tttgaaacac
     1441 ttggtgccga tattgggaat atcccaatat tatggatagc tgctccagtt acaggtttaa
     1501 ttattcaacc catcattgga cacatgagtg ataaaacttg gaacaagcta ggacgtagac
     1561 gtccattctt tttagcaggt gccattttag cctcattagc attaattttt atgcccaata
     1621 gtccgtattt atgggtagct gctggtatgc tttggataat ggatgcctct attaatattt
     1681 cgatggagcc gtttcgagct tttgtgggag atatgttacc tgcagaacaa cgcacaaagg
     1741 gttttgccat gcaaagtttt tttatcggaa caggagcagt agtagctagt gcattacctt
     1801 atatgctaac caattggttt gggattgcca acacagcacc cgaagggctc attcctcctt
     1861 cggtaaaata ctcgttttat ttgggagcta tcgcttttct cgttacagta atatggactg
     1921 ttattagcac aaaagaatat agtccagaag aactcgcaac atttgagaaa aatgaacaaa
     1981 aagagaacga aagtacgagt aatcaccacg aagcaatttt accatcgtct cgttttttaa
     2041 aaatagctcc tatatggact attacgggaa tactattatc agtgttagtt tattttttta
     2101 gtttggaagc ggagttgtat attctgacag ggggtattac atttttcggt cttattctgt
     2161 ttttcgtggg aggcttagta caagccaaca agcaaaaaaa tggattggtt gaagttatta
     2221 acgacctatt gttgatgcct aaaacgatga aacagttggc gattgttcag tttttttcat
     2281 ggtttgccct atttgctatg tggatttata ctacagcggc agtaacatca catatttatg
     2341 gcactagcga tacgcattcg gctatataca atgaaggggc agattgggta ggcattatgt
     2401 ttgcggcata caatggattt gccgctatag ttgccttttt attgccagta ctagccaact
     2461 atacaaacag acgctttacg catgttatag ctttgatagc aggggctgta ggattaattt
     2521 ctatttattt tgttgaaaat cccacaatgc tacttctgcc tatgttagga gtaaggtttg
     2581 gcttggtcaa gtattttatc tatgccttat gctatcttaa cagggtcatt gccatcgcac
     2641 aaaatgggaa catacatggg aatatttaac ttttttattg taattccaca aattatggct
     2701 gctactattt taggattttt tgttacaaaa attgcaggta accatgctat ttatgcctta
     2761 ctactgggcg gcgtttcttt tttgatagca gccgtgatga ctctttttgt aaaagatgaa
     2821 gatgaaaaaa acatgtaact tatatatatt tcaataaaac actacaaaca tgaaaaaatt
     2881 attgctttta gtcggtatca tagcattatt agtagcttgt tcagcacgcg gtatgcttaa
     2941 acttccagta aaatcagaga acaatatgcg gttaaactct ccgctggtgt tggtaaaagg
     3001 aagtaacttt tttttaactc aagactatgt gatagatatt acaacaattg attccataac
     3061 agcagatgct aaaaatggca ttagcgttag cttgtcagcc aacaaagatt cagcgtatat
     3121 caacgcaaca gacatgcctc aattggtaaa tatttcgcta tggagtaaag gagttcctta
     3181 tggtgtatta gccaaaaaaa caaataaaat tgattatgta tttacttatg atgcaactgg
     3241 cgataaaggt acacgttatc aaattgcagg acaaatgaac gattggtttc ctgcaggtca
     3301 tccggattta gtaaaaaacg aaaaaggatt gtacgaagtt actttaaact tgagtcctgg
     3361 tgcctatttg taccaaatgg taattgatgg agactggaac cacgataaaa acaatccaga
     3421 taaggtagat aacaattatg gaaaatttaa ttctatctta catgtgaaag gtacccaaga
     3481 aaaagcacct aaacttctta ctcaatcttt tgatgaaaac aatattgtat tgtctgtaga
     3541 taatgccgca gagcaagtat atgtatactg gcaaaattat cttctaccaa acgatttatt
     3601 tgtaaaaaat aagggacaac aactcattgt aaacctccca aaagaagcta tagaaacaga
     3661 cagatcattt ttacgcatat ttacagctaa cgaatatggt atatcaaacg atgtattaat
     3721 tcctctacaa aaaggcaaag tactaaataa tgtggcggac attacacgtt ttgacaaaca
     3781 tgctgaagct atttactttt tgttagttga ccgttttaaa gatggtaacg aagcaaacaa
     3841 tttccctatg aatcgtccag atgtaaaccc taaagtggat taccaagggg gcgatattgc
     3901 gggtataaca caaaaaatta aagacggtta tttccaacgt ttaggattta ccaccatttg
     3961 ggtatctcct atcgtgcaaa atccaatgga gccatggggt tattgggcaa atccagaaac
     4021 taaattttcg ggctatcatg gctattggcc catctcttcg tctagaattg attttcgttt
     4081 tggtacagat gaagaaatgc acgaattggt taaaacagct cacgaaaaca acataaatat
     4141 aattgtggat tacgtggcta accatgttca taaaaaccac ccactatacc aacgcgattc
     4201 aactattgca acgcctctct atttacctga tggcacaaaa aacacagaaa gatggaatga
     4261 atatcgtttg acaacatggt ttgacgagtt tatgccaagt attgattttt cacgccaaga
     4321 aaacattgat atgatggtgg attcagcact ttattgggtg aaaaaatttg accttgatgg
     4381 ttttcgacac gatgcatgca aacacgtaaa cgaagaattt tggcgtacac ttacacacag
     4441 aatgaaaacg gaagtagaaa ttccaagagg gaaactaacc tatcaaattg gggaatcgta
     4501 tggtagtcct caactcatta gcagctacgt aaatacaggg atgttagatg cacaatttga
     4561 ctttaacgtt tttgatgatg cagcgtcggt atttgccttt aacaacgaag attttgtacg
     4621 tgtgagcaat ggatt
//
LOCUS       DAOPUR010000046         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_18704_length_4628_cov_4.026022, whole genome shotgun sequence.
ACCESSION   DAOPUR010000046 DAOPUR010000000
VERSION     DAOPUR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18704_length_4628_cov_4.026022"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="PKN90_01320"
     CDS             complement(<1..1726)
                     /locus_tag="PKN90_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNZ84955.1"
                     /translation="MIDAKVPSGPLAEKWKNYKAHQKLVNPSNKRRLDIIVVGTGLAG
                     ASAAASLAELGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRARESNVYRLAEVSGSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYAKGQ
                     TGQQLLLGAYSALSRQVGKGAVKLYSRYEMLDVVIIDGRARGIIARNLVSGKIERFFA
                     HAVVVGTGGYGNAFFLSTNAMGSNGSAAIQMYKKGAYFANPCYAQIHPTCIPVHGEFQ
                     SKLTLMSESLRNDGRIWVPKKKEDAEAIRKGTLKPTDLKEEDRDYYLERRYPAFGNLV
                     PRDVASRAAKERCDAGYGVGATGLAVYLDFADSIKRLGLDVVKARYGNLFQMYEKIVD
                     DNPYETPMMIYPAIHYTMGGIWVDYELQTSVKGLFCIGEANFSDHGANRLGASALMQG
                     LADGYFVLPYTIQNYLADQIQVPRMSTDLPEFIEAEKEVNDKIQKLLSIQGQRSVDSI
                     HRELGLVMWDFVGMGRNKEGLQHAIDKIKGIVAKRMPEYWYNNKFNKIKDSDPLEIIR
                     KKKFNQKIV"
     gene            complement(1815..2504)
                     /locus_tag="PKN90_01325"
     CDS             complement(1815..2504)
                     /locus_tag="PKN90_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     cytochrome b subunit"
                     /protein_id="HNZ84956.1"
                     /translation="MWLINSSIGRKLIMSISGIMLVLFLLFHGLMNMVVIFSADAYNA
                     ICAFLGANWYALIATKGLALGFMVHILYAMYLSFQNLKARGKERYAVTDKQDKVSWAS
                     QNMLVLGLIVFGFLALHLYHFWSKMQLVEVGHMLGKHVGDIDKAVDGAYWITYWFSQP
                     VYAILYLVWLTALWFHLTHGVWSSLHSLGLNNQTWLPRVKCISNIFATLVVLLFVSVV
                     VYTFVYQNFVL"
     gene            complement(2796..3953)
                     /locus_tag="PKN90_01330"
     CDS             complement(2796..3953)
                     /locus_tag="PKN90_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84957.1"
                     /translation="MLFLHEIKEMGMKHLEIRTIEQVKSLFHTTDNIQYVAFQHIDFN
                     DVSTSKFDKSYSNCLFLGCILSQEFICKKLRDCTIFPSIDVPYTIFIPHLYTPHDLYA
                     GYKIGSPDSFSHTFDQQVYSHYIAKGKETSDIKETLARRIHDHSISDALHDFLVSYEP
                     TKLVAIMGGHNITRTDIGFLEIAKMSKTLTEQSYLMLSGGGPGAMEATHFGAWMAGRT
                     ESELLHAYEMLSEAPHYDHLLWLEKAFMVMERYPQYTYESIGIPTWFYGHEPPTPFAT
                     RIAKYFENSIREDGLLAIAKGGIIFTPGNAGTIQEIFQDANQNHYLTFGLASPMIFYN
                     ADYWTHSRPIYPLLTQLFEEGKYKNLILSVCNNQQEVLEQLAAFALSQQAE"
     gene            complement(4054..4512)
                     /locus_tag="PKN90_01335"
     CDS             complement(4054..4512)
                     /locus_tag="PKN90_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84958.1"
                     /translation="MKKILILLLLLYFSCFYTFSATFDENRIATGKNKFGFAVGYGNQ
                     LFWDVTYNYQLLLYSLQYQYTFYQKNSFSSELVIQPQYNAASFFEDTQKYSFEYGVNI
                     GALLRKELSNFSVFAMLSAGPHYVSDVPSRQANGFIFSDNLMVGVSYITY"
BASE COUNT         1588 a          923 c          659 g         1458 t
ORIGIN      
        1 caactatttt ttgattaaat tttttctttc ttataatttc aagaggatca ctatctttaa
       61 ttttattaaa tttattatta taccaatatt caggcattct ttttgcaaca atacctttaa
      121 ttttatcaat agcatgttgc aatccttctt tatttctgcc cattcccaca aagtcccaca
      181 taacaagacc tagctctcta tgtatactat ctacagaacg ttgaccttgt atgcttaata
      241 atttttgaat cttgtcattc acctcttttt ctgcttcaat aaattcaggc aaatcagtac
      301 tcattcgtgg tacttgaatt tgatctgcca aatagttttg aattgtataa ggtaatacaa
      361 aatacccatc tgctaaacct tgcattaaag cagatgcacc taaacggttt gctccatggt
      421 ctgagaaatt tgcttcgcca atacagaaca atcctttaac cgatgtttgt agttcataat
      481 ctacccaaat tcctcccatt gtatagtgta tggcaggata aatcatcatt ggagtttcat
      541 atggattgtc atctacaatc ttttcgtaca tttgaaataa gttaccatat ctagccttaa
      601 caacatcaag acctaaacgt ttaattgaat cagcaaaatc taaatataca gctaaacctg
      661 tagcaccaac accatatcca gcatcacatc tttctttggc agcacgagaa gccacatcac
      721 gaggcaccaa attaccaaaa gcaggataac gacgctctaa ataataatct ctatcttctt
      781 cttttaaatc agtaggtttt aaagtccctt tgcgaatagc ttctgcatct tctttctttt
      841 ttggcaccca tatacgacca tcattacgca acgattccga catcaatgtt aatttcgatt
      901 gaaactcacc atgtacagga atacatgttg ggtgaatttg ggcataacat ggattagcaa
      961 aatacgctcc ttttttatac atttgaatag cagcagaccc attagatccc atcgcattgg
     1021 tcgacaaaaa gaaagcatta ccataaccac ctgtccctac tactaccgca tgggcaaaaa
     1081 agcgttcaat ttttccagaa actaaattac gtgcaatgat tcctcgagct cttccatcaa
     1141 taattactac atctaacatt tcgtaacgcg aatatagctt cacggctcct ttgccaactt
     1201 gacggcttag tgctgaatat gcccctaata ataattgttg accagtttga cctttagcat
     1261 aaaaagtacg tgatacctgt gctcctccaa acgaacgatt atctaacaaa cctccatatt
     1321 cacgtgcaaa aggaactcct tgtgcaacac attggtcaat aatacttcct gatacttctg
     1381 ccaaacgata aacattggac tctcttgctc tataatcacc acctttaatt gtgtcataaa
     1441 acaaacgata cactgaatct ccatcatttt gataattttt agcagcattt atacctcctt
     1501 gtgcagcaat agaatgggct ctccttggtg aatcttgaat acaaaagttt agtacgttaa
     1561 aacctaattc agccaacgat gcagcagctg aggctcctgc taacccagta cctacaacaa
     1621 tgatatctaa tcgcctttta ttggatggat ttaccaattt ttgatgtgcc ttgtaattct
     1681 tccatttctc agctaaagga ccagaaggca ctttggcatc aatcataatt ggtgtttctg
     1741 taggctttac agcctgagtt gtttctacaa cagtttcttt cttagccata ttgtgtgata
     1801 tttctagtta caaattaaag aacaaaattt tgatatacaa atgtataaac aaccacagat
     1861 acaaacaata acactaccaa agtagcaaat atgtttgaaa tacattttac acggggtaac
     1921 caagtttggt tattcaaacc caatgaatgc aaagaactcc atacaccatg agttaagtga
     1981 aaccacaatg cagtaagcca taccaaatac aagatagcat acactggttg agaaaaccaa
     2041 taggtaatcc aatatgctcc atcaaccgct ttatcaatat ctcctacgtg ttttcctaac
     2101 atgtgcccaa cttcaactaa ttgcatttta ctccaaaaat gatacaaatg caatgctaaa
     2161 aatccaaata caatcaatcc taaaaccaac atattttgag aagcccatga tactttatct
     2221 tgtttatctg tcactgcata gcgttcctta cctcttgctt ttaaattttg gaaagacaaa
     2281 tacatagcat ataaaatatg aaccataaag cctaaagcca atcctttagt agcaattaaa
     2341 gcataccaat tcgcacccaa aaatgcacaa attgcattat atgcatcagc agaaaaaatc
     2401 actaccatgt tcattagacc atgaaacaac aaaaaaagta ccagcatgat gccagagata
     2461 ctcataatca gctttcttcc tatcgaagag ttaattaacc acatacttcc agttttttaa
     2521 ttattattga tttaatacaa aaatttaatt ctcagtgtac aaaagtaata aaaagttcaa
     2581 tgctaaaggg tatatactat cacatttttt aacgtaatgc aaacgatttt tcattcttgt
     2641 agtaattctc ccaaaaatag tttaagttat tgttatttaa gctaatataa aatcaatttg
     2701 tagcccaaaa aactgtattg ctttcattaa gcatacaaaa ataggtaaat atatgtaaaa
     2761 tttgctcaaa ataacacaac aagcaggata ttatgctact cagcttgttg gctcagagca
     2821 aaagcggcta actgctccaa cacctcttgt tgattattac atacagaaag aataagattt
     2881 ttatacttac cttcttcaaa caattgtgtt aagagcgggt atattggtcg gctatgagtc
     2941 caataatcag cattataaaa tatcattgga cttgctaatc caaaggtaag ataatggttt
     3001 tggttggcat cttgaaaaat ctcttgaata gttcctgcat ttcctggggt aaaaattata
     3061 cctccttttg ctattgccaa caaaccatct tctcgtatac tattttcaaa atactttgca
     3121 atacgtgttg caaaaggagt tggaggttca tgtccataaa accatgttgg gataccaatg
     3181 ctttcgtatg tgtattgtgg gtatctctcc attaccataa acgccttctc taaccataaa
     3241 agatgatcgt aatggggagc ctctgaaagc atttcataag catgtagtaa ttcgctttct
     3301 gttctaccag ccatccaagc accaaaatga gttgcttcca ttgcccctgg acctcctcca
     3361 gataacataa ggtaagattg ctctgtcaaa gtcttactca tttttgctat ttctaaaaat
     3421 ccaatatctg ttcgtgttat attatgacca cccattattg caactaattt ggtaggttca
     3481 taagaaacta aaaagtcatg taatgcgtca gaaattgaat ggtcgtgtat cctacgtgct
     3541 aatgtttcct taatatctga agtctccttc cctttggcta tgtaatggga atatacttgt
     3601 tgatcaaacg tatgcgaaaa tgaatcagga cttccaattt tatatcctgc ataaagatca
     3661 tgtggagtat ataagtgtgg aataaaaata gtataaggga catctattga agggaaaata
     3721 gtgcaatctc gtaatttctt acagataaat tcttgagaaa gaatacaacc tagaaacaaa
     3781 caatttgaat acgacttatc aaatttacta gtacttacat cattaaaatc aatgtgttga
     3841 aaagccacat attgaatatt atcagtagta tgaaagagag attttacttg ttcgattgta
     3901 cgaatctcca aatgcttcat gcccatttct tttatttcat gtaaaaataa caaaaatatt
     3961 ggacttaatc gtacaaatta tggtaaaaca aagaatccta tttgaaaaat agtattatta
     4021 acacctccat taggggcgat gaaaccggca ttactaatat gttatataag aaactccaac
     4081 cattaaattg tcagaaaaaa taaacccatt ggcttgtctt gatggaacat cagaaacata
     4141 atgaggacca gcactaagca ttgcaaaaac agaaaaattt gacaattctt ttcttagtaa
     4201 tgctcctata ttcacaccat actcaaatga atatttttgt gtatcctcaa aaaaactcgc
     4261 agcattatat tgtggctgta ttacaagttc acttgaaaat gaattctttt gataaaatgt
     4321 atactgatac tgaagagaat acaatagtaa ttgataatta taggttacat cccaaaacaa
     4381 ttggttacca taaccaactg caaatccaaa tttattttta ccagttgcaa tacgattctc
     4441 atcaaaagta gcagagaatg tatagaaaca tgaaaaataa agtaaaagca aaagtattaa
     4501 aatcttcttc atttcactat tgttacccct aaataaaaaa aggaggcgat gtatgacaca
     4561 tcacctcctt ttgaaaaacg gcggctatct actctcccac acaagtgcag taccatcgac
     4621 gctattgg
//
LOCUS       DAOPUR010000047         4622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_18732_length_4622_cov_3.462229, whole genome shotgun sequence.
ACCESSION   DAOPUR010000047 DAOPUR010000000
VERSION     DAOPUR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18732_length_4622_cov_3.462229"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1176
                     /gene="aroB"
                     /locus_tag="PKN90_01340"
     CDS             112..1176
                     /gene="aroB"
                     /locus_tag="PKN90_01340"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNZ84959.1"
                     /translation="MSQQRLVFCSTIADSIVAELENKPSTSIFILTDSQSKSFCLPLL
                     QQSETLKNAHVIEVPAGDGSKDVNTLVSIWQYLSENGATRKSLMINLGGGMITDLGGF
                     AASTFKRGMNYVNVPTTLLAMVDAAVGGKTGINFNGLKNEVGVINPAKSVLIDTNFLR
                     TLDMQNLLSGYAEMIKHALISSENELKNIFELDFAQLDYEKLNQLVQSSIYIKEAIVT
                     EDPQEEGVRKALNLGHTIGHAFESYSYQMNKPALHGYAVAWGLMCELFLSYKKLGFPK
                     DTLIKVARYVKENYGHFYISCDDYDALYELMLHDKKNTIANCINFSLLSNVGVLSLNQ
                     HIDKETIFESLDFFQEMMGM"
     gene            1203..2696
                     /locus_tag="PKN90_01345"
     CDS             1203..2696
                     /locus_tag="PKN90_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNZ84960.1"
                     /translation="MKSYLQHIPLGTSLPVSNPHAISVSFPSMDDVIGYEKKDVCTLQ
                     QLQSGYPRFFRNKLVHLLSEIVRKEFKLPVSVEIYPLPCNAVLSLLNLYFCKDFVKID
                     AYGVTFLLLDENDVQLGKLRGFLRQVGVFMSSRNAEDILHSNGLISTTFNEAYTEVQP
                     YYFVQQSLQKAYETNSVDDIILSNCGMNAFFAVFQTIQDTSLLSGKNRVLQLGNLYLD
                     SYDILRTYANKITLSHVTDFDEIRRMIEMHHTVLSAVYVEVPNNPLLECVDLPRLYAL
                     CQEYNIVLLVDSSLASAHTLKLLPYCDVITESLSKFANGQGDVLMGAIVVNAASTYSK
                     EIKSRIASYVIPPYVRDVQRLAHSISDYYERVCLSSASTRKLIEYLENSPEVEQVYSV
                     LHPLSKRNFSILQKNDSLIPSVFSVLFKQPLAYYYDKLPLPKGPSFGTVFTLAMPYVY
                     LAHFDLIQTEEGRQQLTEMGLHPDMLRISVGLEPVEDIIASFKLAGI"
     gene            complement(2715..3473)
                     /locus_tag="PKN90_01350"
     CDS             complement(2715..3473)
                     /locus_tag="PKN90_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNZ84961.1"
                     /translation="MKKIKEFINNSRQNIHSHFRKTYLYKNTNPDLVKHLKPTITCQK
                     KWYGTSYGGFYINPNLLTNQSVIYSFGIGKDISFDMACSNHHQSSIYAFDPTPKSIKW
                     IKEQKLPANFHFFDFGISVGDQEFTVFYLPKNTKSVSGSTIQSDVFDTNNSIKLPMKS
                     FAKITEMLHHTHIDVLKIDIEGSEYEVLEDILTTKISINQLLIEFHDRLFDMQTYKSV
                     EIVKKLNEAGYHIFGCSKSFEEVSFIHASVLKNT"
     gene            complement(3487..4395)
                     /locus_tag="PKN90_01355"
     CDS             complement(3487..4395)
                     /locus_tag="PKN90_01355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ84962.1"
                     /translation="MPTISVIITTFNGSKNISNTLRAILQQEGINSLFEIDIIAIDDC
                     STDNTLEILNEYPIRIFSTETNSGGPNKGRNIGLANAKGDYICIVDQDDVWHTNRISS
                     VLPYIQKAPIISSGYTLIDTSTGKTIERVAQSKEPFIFFEKNSTFLSKLTRSSQGQNT
                     YLGSLLFHRSLRNILFEEHFGKIDYDWVLRLFHQQNSIEICSSLYTRVVDGTNLSLNP
                     TYRRQDFYYSLYFIEQFENLYPTQTRIARKKIHGSRARYYYIMNDMRKARLYFRLAGF
                     SFKNILYYCTTFAGSSFVKKQFNVFG"
BASE COUNT         1422 a          697 c          878 g         1625 t
ORIGIN      
        1 cttaatgccg ttcaaatagc agaataccta attgcagaag gtaatattta atcataagat
       61 actaaacggg tgccaaaaag cattcgttta gtttttaata ctattctaat tatgtcgcaa
      121 caacgcttag ttttctgctc tactattgca gatagcattg tggctgaact tgaaaataag
      181 ccttcaacat ctatttttat tcttactgat agtcagtcca aaagtttttg tttacccttg
      241 ttacaacaga gcgaaacact gaaaaatgct catgttatag aagtgccagc aggcgatgga
      301 agtaaagatg tgaatacgct agtttctatt tggcaatatt taagcgaaaa tggtgctaca
      361 cgaaaatcgt tgatgataaa tttaggagga ggtatgataa ccgatttggg tggatttgct
      421 gcttctacgt ttaaaagagg tatgaactat gtaaatgtgc ctactacctt attggctatg
      481 gttgatgctg cagttggagg taaaacgggt attaacttta atgggttgaa gaatgaagtt
      541 ggggtaatta atcccgctaa gagtgtgttg attgatacaa actttttgcg tacgcttgat
      601 atgcagaatt tgttatctgg ctatgcagaa atgattaaac atgcattgat atcttcggaa
      661 aatgaattaa agaatatttt tgaattagat tttgctcaat tggattacga aaaactcaat
      721 cagttggtgc aatcttctat atatattaaa gaagccatag taacagaaga cccacaagag
      781 gaaggtgtac gtaaagcctt aaatttaggt catactatcg gacatgcttt tgaaagctat
      841 tcgtatcaaa tgaacaaacc cgcattgcat ggttatgctg tagcttgggg tttgatgtgc
      901 gagttgttcc tttcttataa aaaattaggt tttccaaaag ataccttaat taaggtggct
      961 cgttatgtga aagagaatta tggacatttt tacatttcgt gtgatgatta cgatgcattg
     1021 tacgaactaa tgctacacga taaaaagaat acaatagcta attgtataaa ttttagccta
     1081 ttatctaatg taggggtgtt gtcattaaat caacatatcg ataaagaaac gatttttgag
     1141 tcgctcgact tttttcaaga aatgatgggt atgtaatttc ctatacaagg cgtttttata
     1201 caatgaaatc atatttacaa catataccct tgggtacttc gttgcctgtt agcaatcctc
     1261 atgccatttc tgtgtctttt cctagcatgg atgatgtaat tggttacgaa aagaaagatg
     1321 tatgtaccct tcagcagctt caatcaggat atcctcgttt ttttagaaat aagctcgtgc
     1381 atctgctttc cgaaatagta cgtaaagagt ttaaattgcc cgtgagtgta gagatatatc
     1441 ctttgccatg taatgctgta ttatcattgc taaatttgta tttttgtaaa gattttgtaa
     1501 agatagacgc atatggagtt acatttttat tactagatga aaatgatgtt cagttaggta
     1561 aattgcgagg gtttcttcgc caggtgggtg tctttatgtc ttcgcgtaat gcggaggata
     1621 ttttgcactc caatggatta atttcaacaa cgtttaacga agcctataca gaagtacagc
     1681 cttattattt tgtacaacaa tcccttcaaa aagcttacga aacaaactct gtagacgata
     1741 ttattttgag taattgtggt atgaatgctt tttttgctgt atttcaaact attcaggata
     1801 ctagtttgtt atctggtaaa aatagggtat tgcaattggg taatctatac cttgattctt
     1861 atgatatatt acgcacctat gctaataaaa ttaccttgtc tcatgtaacc gattttgacg
     1921 aaataagacg aatgattgaa atgcatcaca cagtgttaag tgcggtgtat gtagaagtgc
     1981 ctaataatcc cttgcttgag tgcgtagatt tacctcgttt gtatgcattg tgtcaagaat
     2041 ataacattgt tttattggtc gattcttctt tggcttctgc ccatactttg aaacttctgc
     2101 catattgtga tgtaataaca gagagcttat cgaagtttgc caacggacaa ggagatgtgc
     2161 tgatgggagc tatagttgtg aatgcagcga gtacgtatag taaagaaata aaatctcgaa
     2221 ttgcgtctta tgttatacct ccttatgtgc gagatgtgca acgattagcc cattcgattt
     2281 ctgattatta cgaaagggta tgtttgtcat cggcaagtac acgtaaattg attgagtatt
     2341 tggaaaattc gcccgaagta gagcaagtat attccgtttt gcacccattg tctaagcgta
     2401 atttttcgat tctacagaaa aatgactcgc ttataccaag tgttttttct gttttgttta
     2461 agcaaccatt agcctattat tacgacaaat tacctttgcc caaaggtcca agctttggta
     2521 cagtctttac tttagctatg ccttatgtat atttagccca ttttgattta atacaaacag
     2581 aagagggtag acagcagtta acggagatgg gacttcatcc tgacatgcta cgaatatctg
     2641 taggattaga acctgttgag gatattattg cttctttcaa gcttgctggg atatagagtg
     2701 attttgtacg aattttatgt attttttaat acagatgcat gtataaatga tacttcttca
     2761 aaactttttg agcaaccaaa aatgtgatat cctgcttcgt tcagtttttt tactatctct
     2821 actgacttat atgtttgcat atcaaatagt ctgtcgtgaa actcaattaa caattgattg
     2881 atggagattt ttgttgttaa aatatcttct aaaacctcat attcagaccc ttctatatca
     2941 atctttaata cgtcgatatg agtatgatgt aacatttctg ttatttttgc gaaagatttc
     3001 attggaagtt ttatgctatt gttggtgtca aatacatctg attgaatagt acttcccgac
     3061 acgcttttgg tatttttggg taaatagaat acagtaaatt cttgatcccc aacggagatg
     3121 ccaaaatcaa aaaaatgaaa gtttgcaggc aatttttgtt ccttaatcca ttttattgat
     3181 ttaggtgttg gatcaaatgc atatatcgat gattggtgat ggttgctaca tgccatatca
     3241 aagctaatgt ctttaccaat accaaatgag taaataacgg attggtttgt aagcaagttg
     3301 gggttgatgt aaaaacctcc ataactagtt ccataccatt tcttttgaca cgttatggtg
     3361 ggttttaggt gtttaactaa atccggattt gtgtttttat aaagatatgt tttacgaaag
     3421 tgagagtgaa tattttgtcg actattgttg atgaattctt ttattttttt catgttttct
     3481 tactgattat ccaaatacat taaattgttt tttaacaaaa ctactgcctg caaaggttgt
     3541 gcaatagtag agtatatttt tgaaagaaaa tcctgctagc ctgaaatata agcgagcctt
     3601 tctcatgtca ttcataatat aatagtagcg agctcgcgaa ccatgaatct tttttcgagc
     3661 aatacgtgtt tgtgttgggt ataggttttc aaattgttcg ataaaataaa gcgaataata
     3721 gaagtcttga cgcctatagg ttgggtttaa tgataggttt gtgccatcta ctacgcgtgt
     3781 gtataacgaa gagcatatct caatagaatt ttgttgatga aacagacgca atacccaatc
     3841 gtagtctatc ttaccaaagt gttcctcaaa caaaatatta cgtaaagaac gatgaaaaag
     3901 cagtgaacca agataggtgt tttgaccttg actggatcgt gtcagtttgc ttaaaaaggt
     3961 gctatttttc tcgaaaaaaa taaatggttc tttcgattgt gccacacgtt caattgtttt
     4021 tccagtagaa gtgtcgataa gtgtatagcc agagctaatg atgggagctt tttggatata
     4081 tggtaatacg ctactaattc gattggtatg ccacacatcg tcttgatcta caatgcagat
     4141 gtaatcaccc ttagcattag caagaccaat attccgtcct ttattcggac cgcctgagtt
     4201 tgtctcagta gaaaaaatgc gaataggata ttcgtttaag atttcgagcg tattgtcagt
     4261 cgaacaatcg tcgatagcga taatatcaat ttcaaataaa ctatttatac cttcttgctg
     4321 taaaatagct cgaagggtgt tacttatgtt tttagacccg ttgaaagtcg taataataac
     4381 tgaaattgtg ggcataattt gtaatattta ctgagtaaaa gtacaagatt aatttgaatt
     4441 aataaattaa tcttgaataa attgtttgta atttgactat tatgttaggt atatatgtgt
     4501 atttatgaaa ttttacacaa aatatatagt agattagcct catttatttt taatatctta
     4561 ctatctttgc tgaataattt gttctaaaat ggtgcatcga aaaatattat tatttacttg
     4621 at
//
LOCUS       DAOPUR010000048         4621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_18740_length_4621_cov_3.294131, whole genome shotgun sequence.
ACCESSION   DAOPUR010000048 DAOPUR010000000
VERSION     DAOPUR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18740_length_4621_cov_3.294131"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKN90_01360"
     CDS             complement(<1..419)
                     /locus_tag="PKN90_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNZ84963.1"
                     /translation="MERNKFIGMGVALVTPFKTDGSVDFSALARLVDYQLKNGTDYLV
                     VLGTTAETPTLSAEEQLEITQFIVEKVNGKLPIVLGMGGNDTRALVQKISTANLNGVD
                     AILSVTPYYNKPTQEGIYQHYAAVAKSTKLPIILYNVP"
     gene            complement(533..1072)
                     /locus_tag="PKN90_01365"
     CDS             complement(533..1072)
                     /locus_tag="PKN90_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84964.1"
                     /translation="MNQLQNILFKLRRYFLLKFHKKQWRDKKYLSFDNISSVALLYYI
                     DDYSKYEIVYKNIKDFTAMGKTVFSVLFVKKGVLAEEKRINMLLFTQNDCSWLCVPND
                     NIMLDALNMSADILIDISLMDSIPLQYIVANSRVGCKVAMKKENKFLYDLMIEMKEEQ
                     VNEDKLLETIAFYLKQIKS"
     gene            complement(1117..3132)
                     /gene="ligA"
                     /locus_tag="PKN90_01370"
     CDS             complement(1117..3132)
                     /gene="ligA"
                     /locus_tag="PKN90_01370"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNZ84965.1"
                     /translation="MNEIHQKILHLRKQLHQYNYEYYVLSQPTISDIEFDFLLKNLQA
                     LEELYPEYYDSNSPTQRVGSDSTTSFVQVSHQYPMLSLSNTYSEGEVRDFYERVSKSI
                     DEPFELVCELKFDGTSISLIYENGVLKQAITRGDGEKGDDVTNNVKTIRTIPLVLHGN
                     QYPAFFEVRGEIVMPWEVFDMLNKEREEQDEPLFANPRNATSGTIKLQNSSLVAKRNL
                     DAYFYAVLGNNLPANTHYDNLLRAREYGFKVSTNTKKCSSLQEVLEYIEYWDIARKQL
                     PVATDGIVIKVNSLTQQRFLGLTAKSPRWAIAYKFQAEKAVTRLNSVSYQVGRTGAVT
                     PVANLDPVLLSGTIVKRASLHNASIIASLDLYVGDSVFVEKGGEIIPKITGVDLDARI
                     LIGSKIQFITHCPECGSKLVNLPGEAAHYCPNEDDCPPQIKGKIEHFVSRKAMNIDGL
                     GKETIDLLYQQGLVKKVSDLYDLSAEQLSVLERLGEKSANNIIEAIEHTKQIPFERVL
                     FALGIRFVGETVAKKLAMAFHSIDKIQEASIQELVEVDEIGERIAQSVYEYLHNNKHQ
                     QLIQKLKDYGLQFTLAEDKLALKSTILAGKKFVISGTFSQFSRDELKDLIENNGGKNI
                     GSVSSQVDFIVAGDNMGPAKLAKAQKLNITLLTEQEFMAMLENGKTI"
     gene            3342..4016
                     /gene="trmD"
                     /locus_tag="PKN90_01375"
     CDS             3342..4016
                     /gene="trmD"
                     /locus_tag="PKN90_01375"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNZ84966.1"
                     /translation="MRIDIITVLPELIESNFSHSIIKRGIEKGLAEVHIHNLRDYSTN
                     KHKRVDDYPFGGEAGMVLQIEPIDKVITELKNQRTYDEIIFTSPDGETFNQKMANELS
                     LVNNIIILCGHYKGVDHRVREHLITKEISIGDYVLTGGELAASIISDAIIRIIPGVIS
                     DEQSALSDCFQDNLLSAPVYTRPAIYNGWKIPEILLSGHEKKISEWRMEQALNRTKKL
                     RPDLLK"
     gene            complement(4021..>4621)
                     /locus_tag="PKN90_01380"
     CDS             complement(4021..>4621)
                     /locus_tag="PKN90_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNZ84967.1"
                     /translation="FLQTNEDVFRLTSKFGYKAFTKFYYTGQTEFTTQLFNTYKVNTY
                     DRITGAFSPIRLSVSGGLDYKESADFSIFFSPVSYKYIYVNDTTKHISVAQSIADKLG
                     IDKGKKSLHEVGSLVRINYRYKLSNEIEFDTRTTIYTNYKGIEFDSEVNGNFKINRFL
                     TTRLSVNPRYDSTFKLPNNDKARLQFRQILSFGFSYKFY"
BASE COUNT         1519 a          814 c          665 g         1623 t
ORIGIN      
        1 ggaacattat acaatatgat aggcaatttt gtacttttgg ctactgctgc atagtgttgg
       61 taaatacctt cttgtgtagg tttgttataa taaggagtta ctgataaaat tgcatcaaca
      121 ccatttaaat ttgctgttga aattttttgt accaacgcac gtgtatcatt tccgcccata
      181 cccaatacga taggtaactt tccatttact ttttccacaa taaactgagt tatttctaac
      241 tgttcttcag cagataaggt aggagtttca gctgttgtac ctaacacaac taaataatca
      301 gttccatttt ttagctgata atcaactaat cgagctaaag ctgaaaaatc aacactgcca
      361 tccgttttaa aaggtgttac caaagcaaca cccattccta taaatttatt gcgttccatt
      421 aaaaagtgta ttttttcttt aagttgcaaa aatagtattt tttattcaaa aacagctact
      481 ataagaaagg atttttggga attttgtaca atgtataatc aattattata cattacgatt
      541 taatctgttt taaatagaaa gcaattgttt ctaaaagttt atcctcatta acctgttcct
      601 ctttcatttc aatcattaaa tcgtataaaa atttattttc ttttttcatt gcaaccttac
      661 aaccaacccg tgagttagct actatatatt gtaaaggaat actatccata agtgatatat
      721 ctattaatat atctgcagac atattcaacg catcaagcat aatattatcg ttaggaacac
      781 aaagccatga acaatcattt tgggtaaaga gtaacatatt gatgcgtttc tcttctgcaa
      841 gtaccccttt ttttacaaac agtacagaaa acactgtttt tcccattgct gtaaaatctt
      901 taatgttttt gtatactatt tcatatttac tataatcatc tatgtaatag agtaatgcaa
      961 cagaagatat gttgtcgaat gaaaggtatt ttttatctct ccattgtttt ttatgaaatt
     1021 tgagtaaaaa atatcttcgt aatttgaaaa gaatgttttg taattgattc atctttttta
     1081 gtataatttt tgccttatta aataatgcta ttttttttaa atagttttgc cattttcgag
     1141 cattgccata aactcttgtt ccgttagtaa tgtaatgttt aatttttgtg ctttagccaa
     1201 cttagcaggt cccatattgt ctccagccac aataaaatca acctgagagg atacagaccc
     1261 aatattttta cctccattat tttcaatcaa atcttttagc tcatcacgcg aaaactgaga
     1321 gaaagtgcct gatataacaa attttttacc agctaatata gttgatttta aagctaattt
     1381 atcctcagca agtgtaaact gtaaaccata atcttttaat ttttgtatca attgttgatg
     1441 tttgttattg tgtaaatact catatacact ttgagcaatc ctttctccta tttcatcaac
     1501 ttcaactaac tcctgtatac tagcttcttg tatcttatca atagaatgaa atgccatagc
     1561 tagtttttta gcaaccgttt cacctacaaa acgaatgcca agggcaaata atacacgttc
     1621 aaaaggaatt tgttttgtat gttcaatagc ttctattata ttgttggcag atttttcgcc
     1681 taatcgctct aatacagaca attgttctgc tgataaatca tataaatctg ataccttttt
     1741 tactaaacct tgttgataca ataaatcaat ggtttctttt cccaaaccat caatattcat
     1801 agctttccgg ctaacaaaat gttcaatttt tcccttaatt tgtggtggac aatcatcttc
     1861 atttgggcaa taatgtgcag cctccccagg taaattcact aacttagagc cacactctgg
     1921 acaatgagta ataaattgaa tcttactgcc tatcaaaatt cgtgcatcta aatctactcc
     1981 tgttatttta gggattatct cccctccttt ttctacaaat acgctatcac ctacatataa
     2041 atctaaagag gcaataatac ttgcattgtg tagcgatgct cttttaacaa tagttcctga
     2101 caataacact ggatctaaat tggcaacagg tgttacagct ccagttctac ctacctgata
     2161 tgacacagaa ttgagcctag taactgcctt ttctgcctga aacttataag caattgccca
     2221 tcgaggggat ttggcagtta atcccaagaa acgttgctgt gttaatgaat tgactttaat
     2281 aacaattcca tcagttgcca cagggagttg tttacgagca atatcccaat actctatata
     2341 ctctaaaact tcttgtaagg aactgcactt tttagtgttt gtagatactt taaacccata
     2401 ctcacgagct cttaataaat tatcataatg agtattagca ggcaagttat ttcccaaaac
     2461 agcataaaaa taagcatcta aatttctttt ggctaccagg gatgaatttt gcaatttaat
     2521 tgttccagag gttgcattac gcggatttgc aaacaaaggt tcgtcttgct cctctcgttc
     2581 tttatttagc atatcaaata cttcccaagg cattacaatc tctcctctta cttcaaaaaa
     2641 agctggatac tgattgccgt gcaacacgag tggaattgta cgtattgttt tcacattgtt
     2701 agttacgtca tctccctttt caccatctcc tcttgtaatg gcttgtttta acactccatt
     2761 ttcgtaaatc aaagagatag atgtcccatc aaatttcaat tcacagacta actcaaatgg
     2821 ctcatcaata gattttgata ccctttcgta aaaatcacgt acttctcctt ctgaatatgt
     2881 atttgataat gataacattg gatattgatg tgaaacttgt acaaaactag ttgtactatc
     2941 acttcctacc cgctgcgtag gagaattact atcatagtat tctggataca attcttctaa
     3001 tgcctgtagg tttttcagta aaaaatcaaa ttcaatatcg gaaattgtag gctgagaaag
     3061 cacataatac tcataattat attgatgtaa ctgtttacgc aagtgcaaaa ttttttgatg
     3121 tatttcgttc attattaaat aattatttaa ctataaagta ataagaaact atttgtttct
     3181 agtatgattc aatagaaatg ctattcagta ttacaactca attgagtaaa caaatgtaat
     3241 aaaaatcgtg ttttgatttg gtattttatg tatatacttt tcaacaatcc acaagtatga
     3301 aaatttttac gtacttttgt cgaaaatcca tttgtagtta tatgcgtata gacatcatta
     3361 cagtattgcc tgaattaatt gaaagcaatt tctctcattc aattatcaaa agaggaatag
     3421 aaaaaggttt ggcagaagta cacatccata atttacgtga ttactctacc aataagcata
     3481 agcgagtaga tgattatccg tttggtggcg aagctggcat ggttttgcaa atcgagccta
     3541 ttgacaaggt aataactgaa ctaaaaaatc aacgtacata cgatgaaata atttttactt
     3601 ctccagatgg ggaaactttt aaccaaaaaa tggcgaacga actttcactt gttaataata
     3661 tcattatact ttgtggtcat tataaaggag tggatcatcg tgtacgagaa catttaataa
     3721 ctaaagaaat ttctatagga gattatgttc tgacaggagg cgaattagct gcatcaataa
     3781 tatcagatgc tatcattcgc ataatacctg gcgttatttc tgacgaacaa tcggcattgt
     3841 ctgattgctt tcaagacaac ttactttctg cccctgttta tacacgtcct gctatttata
     3901 atggatggaa aattcctgaa atattgttat ctggacacga aaaaaaaata agtgaatggc
     3961 gtatggagca agctctaaac agaacaaaaa agctacgtcc agatttacta aagtaacaac
     4021 ttagtaaaat ttatatgaaa atccaaaact caatatttgc ctaaactgta aacgtgcttt
     4081 gtcattattt ggcaacttaa atgttgaatc ataccgtgga tttaccgaga ggcgtgttgt
     4141 tagaaatcta tttattttaa aattgccatt tacttcggag tcaaactcta tacctttata
     4201 gttggtatat atcgtagtac gtgtatcaaa ttcaatctca ttactcaatt tatacctata
     4261 atttatacgc accaaactac ctacttcatg taacgatttc ttccctttat caattcctaa
     4321 tttatctgct attgattgag caacggaaat atgtttcgta gtatcattca cataaatata
     4381 tttataagaa acaggtgaaa aaaatatgga aaaatcggca ctctctttat aatctaaacc
     4441 accacttact gataatctga taggagaaaa agcacctgtg atacgatcat atgtattaac
     4501 cttataggtg ttaaagagtt gtgtcgtaaa ttctgtttga ccagtataat aaaatttagt
     4561 aaatgcttta taaccaaatt tactagtcaa acgaaacaca tcttcatttg tctgaagaaa
     4621 t
//
LOCUS       DAOPUR010000049         4584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_18920_length_4584_cov_3.236918, whole genome shotgun sequence.
ACCESSION   DAOPUR010000049 DAOPUR010000000
VERSION     DAOPUR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18920_length_4584_cov_3.236918"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..411)
                     /locus_tag="PKN90_01385"
                     /pseudo
     CDS             complement(1..411)
                     /locus_tag="PKN90_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480514.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(518..2707)
                     /locus_tag="PKN90_01390"
     CDS             complement(518..2707)
                     /locus_tag="PKN90_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNZ84968.1"
                     /translation="MNTLRFKVLDEAFRRKAVQLPESKERLTDYYAKYVFDRNKMQRY
                     LSKNTYGILTNSIDKGEPLNRSIADGVATGMKQWAMEMGATHYTHWFQPLTGGTAEKH
                     DSFLDFNNIGGVIEDFSGKLLAQQEPDASSFPSGGIRNTFEARGYTAWDPSSPAFVVG
                     DTLCIPTIFISYTGEALDYKTPLLKSIGVLNKAAVDVCKYFDENVSKVTSFLGWEQEY
                     FLVDEALWAARPDLMLTGRTLMGHSSAKNQQLEDHYFGAIPSRVLAFMKDLEIESLKL
                     GIPAKTRHNEVAPNQFELAPIYEESNLANDHNLLLMSVMKKIARRHNFRVLFHEKPFK
                     GINGSGKHNNWSIGTDTGVNLLSPGKTGIENLQFITFMVNVLMAVYKNNALLKASISS
                     ATNAHRLGANEAPPAVVSVFLGKQITNFLDKMEVASTDEAIIFDDKVGIRLGVAHIPE
                     ILLDNTDRNRTSPFAFTGNRFEFRAVGSSANCGGAMMALNTAVAGQLKQFKTEVDALI
                     ASGMLKEKAIVTVLKKYITISKPIRFDGNGYSDEWKIEAAKRGLDCETSVPKIFDAYL
                     SKQSIELFKKTNILSEVELHSRCEVKWETYTKKIQIEARVLGDLAMNHILPLASRYQS
                     LLLDNVSKISTIFSADEAKSMTEQDRTIIKTIANHISEIAKQTTELISARKVANKIES
                     EREKAIAYHDTVIPVMDELRSHIDDLEMIVDDEMWTLPKYRELLFIR"
     gene            complement(2727..3761)
                     /gene="asnA"
                     /locus_tag="PKN90_01395"
     CDS             complement(2727..3761)
                     /gene="asnA"
                     /locus_tag="PKN90_01395"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNZ84969.1"
                     /translation="MLLYIPKNYTPSLDSEKMELAIKQLKDDFQAELTHQLNLRRVTA
                     PLFVLSGTGVNDDLNGTERAVSFPIKDMGNRRAEVVHSLAKWKRMKLGAYNIPAGYGL
                     YTDMNAIRADEELDNLHSLYVDQWDWEQTITQEDRTIEFLKSTVKKIYQALKTIELKL
                     HGKYPHISPTLPSEIRFFHSEELLSLYPKLSPRERETKVAEKYGAVFVIGIGAPLADG
                     KKHDGRAPDYDDWSTPTSKGLRGLNGDIILWSKVLDSAFEISSMGIRVDKKALLYQLK
                     EEGCMNRTELDFHSQLINEKIPLSIGGGIGQSRLCMYFLKCAHIGEVQASLWPEEMIE
                     SCKANKILLK"
     gene            complement(3852..4367)
                     /locus_tag="PKN90_01400"
     CDS             complement(3852..4367)
                     /locus_tag="PKN90_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84970.1"
                     /translation="MHGAGNDYVYINGFEETITSPSAFSIRISDRHFGIGSDGLVAVT
                     AGCDVVSGWGAVAIGLLAGILLVFSLRLIENVFKIDDPVGAASVHGITGAFGTLAVGL
                     FSQSTGLFYGFGTQALLSQFIGVVSVAAWGLGAGFILFFILKKTVGLRIDKRVEEEGL
                     DIYEHGETAYN"
BASE COUNT         1393 a          861 c          753 g         1577 t
ORIGIN      
        1 cgcctaacaa ttgactatta acaggcacat cacgtatggc aagtgtagat aatccttgtt
       61 ctaaagcaat ggtttctaat cggttgagta tatctttttg tactgtattg tttttaggta
      121 aaaacacaag tcctgtacca tatcttcctc tttctggaac ggcaatacct tgtaaaagaa
      181 taaactcgtg aggtatttgc actagtattc cggcaccgtc accggtattt ttctcagccc
      241 cttcagctcc tctgtgtacc atgttttcga gtacttgtag ccctttttta caacaataga
      301 gtgagatttt tcgccgttga tattaacgac taaaccaacc ccacacgcat cgtgttcatt
      361 tattggatca tacagacctt gctgctcagg tataattgtt gcgtttttca tataaatttt
      421 gttaaaaaaa cgagttgtaa atgcctgttt tggtcaagaa ttcttgacca aaataagaca
      481 tgaaaatagt ttatttgcag atataatctg cttaaaatta gcggataaac aataattctc
      541 tatattttgg caatgtccac atttcatcat caacaatcat ttctaaatca tcaatatgag
      601 aacgtagttc gtccattaca ggaattacgg tatcgtggta tgcaattgct ttttctcttt
      661 cagattcaat tttgtttgca accttacgag cacttattag ttctgttgtt tgtttagcaa
      721 tttcgctaat atggttagca attgttttaa taattgtacg gtcttgttca gtcatagatt
      781 ttgcttcgtc agcagagaaa attgtactga tttttgatac attatccaac aataacgatt
      841 gataacgaga agccaatggt aaaatatgat tcatcgctaa atcacctaat acacgagctt
      901 cgatttgaat ttttttagta taggtttccc attttacttc gcaacgagag tgtaattcta
      961 cttcgctcaa aatatttgtc tttttgaata attcaataga ttgtttgctt aaatatgcat
     1021 caaatatttt tggaacgctt gtttcacaat ctaatccacg tttagctgct tctattttcc
     1081 attcgtcgct atatccgttg ccatcaaaac ggattggttt agaaatagtt atgtattttt
     1141 tcaatacagt tacaatagct ttttctttca acataccgct tgcaattaat gcatcaactt
     1201 cggttttaaa ttgtttaagt tggcctgcta ctgcagtatt taatgccatc attgcaccac
     1261 cacagttagc tgatgaacca actgcacgga actcgaaacg gtttccagta aatgcaaaag
     1321 gtgaagtacg gttacggtct gtattgtcaa gtaaaatttc aggaatatga gccacaccta
     1381 atcttatacc tactttgtca tcaaatatga tagcttcgtc ggtacttgct acttccattt
     1441 tgtctaagaa attggtaatt tgttttccta ggaatacaga tacgacagca ggaggagctt
     1501 cgttggcacc taaacgatga gcgttggttg ccgagctgat agaagctttt aataaagcat
     1561 tgtttttgta aacagccatt aatacgttta ccataaaggt aataaattgc aagttttcaa
     1621 ttcctgtttt acctggagaa agcaaattaa cgccggtatc agttccgata gaccagttat
     1681 tatgtttgcc tgaaccatta atgcctttaa aaggtttttc gtggaataaa actctgaaat
     1741 tatgacgacg agcaattttc ttcattaccg acatcaacaa taaattgtgg tcgtttgcta
     1801 agttgctttc ttcataaatt ggagctaact caaattggtt tggagcaacc tcattgtgtc
     1861 ttgtttttgc agggataccc aattttaatg attcaatttc taagtctttc ataaaagcta
     1921 acacgcgact tggaattgct ccaaagtagt ggtcttctaa ttgttggttt ttagcactac
     1981 tgtgtcccat taaggtacgt ccagtaagca ttaagtcagg acgtgctgcc cacaaagcct
     2041 catcaaccaa gaagtattct tgttcccaac ccaaaaagct agttactttg ctcacgtttt
     2101 cgtcaaaata tttacataca tcaacggcag ctttgttcaa tacgccgatt gattttagta
     2161 gaggagtttt gtagtcaaga gcttcacctg tgtacgaaat aaaaatagta gggatacaaa
     2221 gtgtatcgcc aacgacaaaa gcaggagaag atggatccca tgcagtataa cctctagctt
     2281 cgaaagtatt acggatacct ccacttggga aacttgaagc atcaggttct tgttgtgcta
     2341 ataattttcc tgagaaatct tcgatcactc cacctatgtt attgaaatct aagaaagaat
     2401 cgtgtttttc ggctgttccg ccagtaagag gttgaaacca gtgagtataa tgagttgcac
     2461 ccatttccat tgcccattgt ttcatacctg tagctacacc atcagcaatg cttctattta
     2521 gtggttcacc tttatctatt gaattagtta atattccata agtgtttttt gaaaggtaac
     2581 gttgcatctt gttacgatca aacacatact tggcataata gtcagttaaa cgttctttac
     2641 tttcaggtaa ctgaacggct ttgcgtctaa acgcttcatc taatacttta aatcttagtg
     2701 tgttcatatg tgtaaaaaat tagttattat tttaatagaa ttttatttgc tttacatgat
     2761 tcaatcattt cttcgggcca taatgatgct tggacttcgc caatatgtgc acattttaga
     2821 aaatacatac ataatctaga ctgacctatt cctcctccga tagataaagg aattttttca
     2881 ttaattagtt gactatgaaa atctaattcc gtccgattca tgcatccttc ttcttttagt
     2941 tgatacaaga gtgctttttt gtctactcgt attcccatag aggatatttc aaatgcacta
     3001 tctaatactt tactccacaa aatgatgtct ccatttaacc ctcttagtcc tttgcttgtt
     3061 ggagtagacc aatcgtcgta atcaggagct cgtccatcgt gtttttttcc atctgctagt
     3121 ggagctccaa ttcctattac aaaaacagca ccatattttt cagccacttt tgtttctcgt
     3181 tctctagggc taagttttgg gtacaaagat aataattctt ctgaatgaaa aaagcgtatt
     3241 tcactaggta atgtaggcga aatatgtgga tattttccgt gaagtttcaa ttcaattgtt
     3301 ttgagagctt gataaatctt tttaactgtc gattttaaga attcaatagt tctatcttct
     3361 tgggtaattg tttgttccca atcccattga tcaacgtata atgaatgtaa attatccaat
     3421 tcctcatcag ctctaatggc attcatatct gtataaagac catatcctgc aggtatgttg
     3481 taagcaccta atttcatcct tttccatttg gctaacgaat gtactacttc tgcacgtctg
     3541 ttgcccatat cttttattgg aaatgatact gcacgttctg ttccattaag atcatcatta
     3601 actccagttc cagataaaac aaacagtgga gccgtaacac gacgtaggtt tagttgatgc
     3661 gtaagttcag cttgaaaatc atctttcaat tgtttaatag ccaattccat tttttcagaa
     3721 tcaaggctag gggtatagtt ttttgggata taaagtaaca tagttacatc aatttagttt
     3781 gcgttttaga aatcattgaa aaagagagga ctaaggtttt ttcaatagcc ccctctttga
     3841 taaaagttta attagttata agcagtttca ccatgttcat atatatccaa accttcttct
     3901 tctactcgtt tgtcaatacg taaacctact gttttcttta agataaagaa tagaataaat
     3961 cctgcaccta atccccacgc tgctactgaa actaccccaa taaattgtga taatagagct
     4021 tgtgttccaa atccatagaa taaaccagta gattgagaaa ataagccaac agctaatgtg
     4081 ccaaatgcac ctgtaatacc atgtacagag gctgcaccaa cggggtcatc aattttgaat
     4141 acgttttcaa tcaaacgaag tgagaatacc aataatatac ccgctaataa tccgatagct
     4201 acagcacccc atccacttac tacatcgcaa ccagcggtta cagctactaa tccgtctgaa
     4261 ccaattccaa aatgacgatc gctaatacgt atagaaaatg ctgaaggaga tgttattgtt
     4321 tcttcaaaac catttatata tacgtagtcg tttccggctc catgcatctt cgtaaatttg
     4381 attagttttg acataatata ttgtaaatcg attaaataat gacaaaatgt tttgttttca
     4441 atgcaaaagt atgaataatg atattttaag acaaggaaaa tgtgtgtaaa tgctattttc
     4501 attttcaata atatcaaaaa taaactaaat tagctataaa aaaattaata tgaaagcaaa
     4561 atacgtttaa aatgttacat aatg
//
LOCUS       DAOPUR010000050         4572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_18986_length_4572_cov_3.076821, whole genome shotgun sequence.
ACCESSION   DAOPUR010000050 DAOPUR010000000
VERSION     DAOPUR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18986_length_4572_cov_3.076821"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="PKN90_01405"
     CDS             <1..1382
                     /locus_tag="PKN90_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ84971.1"
                     /translation="YTTPHGAHTIGSVGEVNQQQIDADSYYSRGDYKGQSGLEKEYET
                     YLRGEKGVEILLRDAHGRIQGVYKNGERNKAPIAGKNLTMAIDMGLQEYGEQLMIKKL
                     GSIVAIEPSTGEILALISSPSYDPHLLVGRERSKNYNALLKDPFKPLFDRPLMAMYPP
                     GSTFKLVNGLIYQQEGVITEDTRFSCSMGYYYSRTRKLGCHPHPNNLDLRESVQHSCN
                     AYYCHGLKGLLDSPPSGISTTESYNHWRNHVLSMGFGKKLGVDFPNEKGGLILESNRY
                     DRIYGKNKWKSSTIISIAIGQGEVLATPIQTANLAALIANRGYFYTPHIIKKIEGDSI
                     DKRFREKHQTTIEQKYFDPIIDGMLMAVEQGTGRVGRIEGIEICGKTGTAQNPHGKDH
                     SIFMAFAPKENPKIAIAVYVENSGFGATWAVPIASLMIEKYLTGTVDTTKLNRKNLQK
                     RMLETFLIE"
     gene            1395..2867
                     /gene="rodA"
                     /locus_tag="PKN90_01410"
     CDS             1395..2867
                     /gene="rodA"
                     /locus_tag="PKN90_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNZ84972.1"
                     /translation="MSRKVSLFRSLDWITILLYVTLVTIGWFAIYSSTYDFENPTILN
                     FSARHGSQLIWIGFAFLLALITILIDSKMYNNLAYIIYFLIILLLIATIFLAPEIKGS
                     RSWLKIGPITMQPAELAKYATALCLGKFMSSYNFTLNSIKNMFLVGFIILTPMLIIIL
                     QQETGSALVFAAFFLVLFREGMNGIFMVLAFLVALFFVLFLRYDYISSSSNSYILESF
                     GFVIVFILSVISVGIMIAYYLKDNRQMLQFIGGNAILYLLTGILHLFGIEAITYTITA
                     YISISLSILYLFIMALLQKQKKYFLFIIFLFGSIALCNVTDYFFNNVLQPHQQMRIKV
                     VLGLEDDPLGAGYNVRQSKIGIGSGGLLGKGFLNGTQTKLNYVPEQDTDFIFCTIGEE
                     QGFVGSIVVLFLYIALIIRLVILAERQRSSFSRIFGYSVLSIIFFHVAINIGMVIGLV
                     PVIGIPLVFLSYGGSSLWSFTILLFTFIRLDANRMEILHQ"
     gene            3045..3290
                     /locus_tag="PKN90_01415"
     CDS             3045..3290
                     /locus_tag="PKN90_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNZ84973.1"
                     /translation="MNIYIGNLNYRVRENDLKGIFEEYGTLDSVKLVKDRETGKSKGF
                     AFAEMSNDSEAKKAIEELNGAEFEGRTLVVKEANPRN"
     gene            complement(3348..4319)
                     /locus_tag="PKN90_01420"
     CDS             complement(3348..4319)
                     /locus_tag="PKN90_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNZ84974.1"
                     /translation="MNKIDEIKQPIIADLLAYEKELALALQTENPILNQLSRYVLSTR
                     GKQMRPILSLLVAQVCGGITESTIQGTIAIELLHIASLIHDDVLDSALERRNKPSVNA
                     VFENKIAVLGGDYLLSKALSKASNSRNLDIIFEISKVGSNLVDGEILQLANSKKQFIT
                     EDIYFDIISKKTASLFSSCARIGALSANATNHEIDCLGIYGTSIGICFQLRDDIFDYF
                     SDDVGKPTGNDLREGKLTLPMLFALSKATNSEKEYLFSLLRKADLTEKEVQELQQFSI
                     QKGGVEYATKKMYEYRDKAIRQLQHFATSATKNALMEYATYVIERNK"
     gene            complement(4324..>4572)
                     /locus_tag="PKN90_01425"
     CDS             complement(4324..>4572)
                     /locus_tag="PKN90_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNZ84975.1"
                     /translation="TPIGIITDGDVRRAIQKYQDISNLTAYDFMTVGYKSITENERIV
                     DALRIMETNKITTLAVVDVATPTIIGIIHIHDIFAYRK"
BASE COUNT         1494 a          736 c          786 g         1556 t
ORIGIN      
        1 aatacaccac ccctcatgga gctcatacaa ttggctctgt aggagaagta aatcagcaac
       61 aaatagatgc tgattcatat tattctcgag gtgattataa gggtcaatct ggattagaaa
      121 aagaatatga aacgtattta agaggagaaa aaggagtaga aatattatta agagatgctc
      181 atggtcgtat tcaaggggta tataaaaatg gagaaagaaa caaggctcca attgcaggca
      241 aaaatctgac catggctatt gatatggggt tacaagagta tggcgaacaa ctaatgatca
      301 agaaattagg cagtattgta gccattgaac cctctacagg agaaatatta gcccttattt
      361 caagcccatc atatgaccct catttgttgg taggaagaga acgttctaaa aattataatg
      421 cattgctgaa agaccctttc aaacccttat ttgacagacc cctaatggca atgtatcctc
      481 caggatcaac atttaagtta gtaaatggat taatctacca gcaagaaggc gttataaccg
      541 aagacacacg cttttcttgt agcatgggtt actactatag tcggacacgt aaattaggct
      601 gtcatccaca tcctaacaat ttagatttac gcgaatctgt tcaacattcg tgcaatgcgt
      661 attattgtca tggattgaaa ggtttattag attctccacc aagtggtatt tctactaccg
      721 aatcctataa tcattggaga aaccatgttc tatctatggg ttttggaaaa aaattaggag
      781 tcgattttcc taatgaaaag ggaggtttga ttttagaaag taatcgctat gaccgaatat
      841 acggaaaaaa caaatggaaa tcatccacta ttatctctat cgcaattggg caaggagaag
      901 tgttagcaac tcccattcaa acagcaaatt tggcagcact aatagcaaac agaggatatt
      961 tttacactcc tcacattatc aaaaaaattg aaggagatag tattgataaa cgttttagag
     1021 aaaaacatca aacgacaata gaacaaaaat atttcgatcc tattattgat ggaatgttaa
     1081 tggctgtgga acaaggaaca ggtagggttg gcagaataga aggtattgaa atttgtggta
     1141 aaacaggaac ggcacaaaat ccacacggaa aagatcattc aatttttatg gcttttgctc
     1201 cgaaagaaaa tccaaaaata gcaatagctg tatatgtgga aaattcgggt tttggagcaa
     1261 catgggcagt accgatagct agtttaatga tagaaaaata ccttacaggt actgtagata
     1321 caacaaaatt aaaccgtaaa aatcttcaaa aaaggatgtt agaaacattt ttaattgaat
     1381 aaaaaatatc aactatgtct cgtaaagtta gtctttttcg ttcattagat tggattacca
     1441 ttttattgta tgttacattg gtaactatag gatggtttgc tatctactca tcaacatacg
     1501 attttgaaaa tccaactatc ctgaatttta gtgctagaca tggctcccaa ctaatttgga
     1561 ttggttttgc ttttttactg gcacttataa cgatattgat agatagcaaa atgtacaata
     1621 atcttgctta tattatttat tttctaatta ttttactgtt aattgccacc atttttttag
     1681 ccccagaaat taaagggtct cgctcatggc taaaaattgg accgattacg atgcaacctg
     1741 cggaattagc taaatatgct acagctctat gtctcggaaa atttatgagt tcatacaatt
     1801 ttacacttaa ttccatcaaa aatatgtttc ttgttggatt cattatttta actcctatgt
     1861 taatcatcat actccaacaa gaaactggat ctgcattggt ttttgcagca ttctttcttg
     1921 tattgtttag ggaaggcatg aatggaattt ttatggtact agccttctta gtagctttat
     1981 tttttgtcct ttttttacga tatgattata tttcgtcgag tagtaactcc tatatactcg
     2041 aatcgtttgg ttttgtcatc gtattcattt tgtctgttat ttcggtgggc ataatgattg
     2101 cttattacct aaaagataat aggcaaatgt tacaatttat tggtggtaat gctatattat
     2161 atttacttac aggaatctta catctatttg gaatcgaagc tattacatat actataactg
     2221 cttatataag tatatcttta agtattctct atttattcat aatggcttta ttacaaaagc
     2281 aaaagaagta tttcctcttt atcatatttc tgtttggaag tatagccctg tgcaatgtta
     2341 ctgattattt tttcaacaat gtgttgcaac ctcatcagca aatgcgtatt aaagtagtat
     2401 taggattaga agacgaccca ttgggtgctg gatacaatgt tcgtcaatca aaaattggca
     2461 ttggttcagg tgggttgctt ggaaaaggat ttctaaatgg aacacaaaca aaattaaatt
     2521 atgttcccga acaagataca gatttcattt tttgcacaat tggagaagaa caaggatttg
     2581 tgggaagtat agtggtctta tttctatata ttgcattaat catacgactt gtaattttgg
     2641 ctgaacgaca gcgatcttcc tttagtcgta tttttggcta ttctgtgtta agtattattt
     2701 tctttcatgt tgctataaat atcggaatgg ttattggttt agttcccgtt attggtattc
     2761 cgttagtttt tttaagctat ggaggttctt ctttgtggag cttcacaata ttattgttca
     2821 catttatacg tcttgatgct aatagaatgg aaatattaca tcaataactc ctgtgaggta
     2881 tataaaatta taaccgaaac gtaacctaaa taatgaaata tacaacaaaa tccacatttt
     2941 ttttcaaatt atggtatttt attaaaatat tatgctttcc tttgtgtcgt aaatcattaa
     3001 gtgaagattt acgacatatt tatttatttt tttaattttt ttttatgaac atttacattg
     3061 gaaacttgaa ttatcgagtt agagaaaacg acctaaaagg tatctttgaa gaatacggaa
     3121 cattggattc tgtaaaactt gtaaaagaca gagaaacagg aaaatcgaaa ggttttgcat
     3181 ttgcagaaat gtcaaacgac agcgaagcaa aaaaagctat cgaagaactg aatggtgctg
     3241 aatttgaagg aagaacatta gtggttaaag aagcaaatcc tagaaattaa ttgatattat
     3301 ttttatggga attaaaaaga ggctattcaa ttgaatagcc tcttttgtta tttgtttcgt
     3361 tctataacat aggttgcata ttccatcaag gcatttttag ttgcagaagt agcaaaatgt
     3421 tgtaattgac gaattgcttt atctctgtat tcatacattt tcttggttgc atattccact
     3481 cccccttttt gaatggaaaa ttgttgcaat tcttgcactt ctttttcagt caaatctgct
     3541 ttccgaagca aagaaaatag atattctttc tcgctattcg ttgctttact cagagcaaac
     3601 agcataggta aagtaagctt tccttctcga agatcatttc ctgtaggctt gcctacatca
     3661 tccgaaaaat aatcaaatat atcgtctctt aattgaaaac atatcccaat gcttgtacca
     3721 taaataccta aacaatcaat ttcgtgattg gtagcatttg ctgatagtgc tcctatgcgg
     3781 gcacaactag aaaaaagact agctgtcttt tttgaaataa tatcaaaata gatatcctcg
     3841 gttataaatt gctttttaga atttgccagc tgcaaaatct ctccatccac cagatttgat
     3901 cctactttag atatttcaaa gataatatct aagtttctac tgtttgaagc cttggataag
     3961 gctttagata acaaataatc tccacccaat acggcaatct tgttttcaaa caccgcgttt
     4021 actgatggtt tgtttcttcg ctctaaggca ctatcaagta catcatcatg aattaaacta
     4081 gctatatgta gcaactcaat agcaatagta ccttgtatag ttgattcagt aatgccgcca
     4141 cagacctgag caactaacaa agacaaaata ggtcgcattt gtttacccct cgtgcttaat
     4201 acgtagcgtg acaattgatt gagaattgga ttttctgttt gaagagctaa ggctagttct
     4261 ttttcatatg ccaacaaatc tgcaattatt ggttgtttaa tttcgtctat tttgttcata
     4321 aacttatttt ctgtatgcga aaatatcatg tatatgtata atgccaataa tggttggcgt
     4381 agcaacatct actacagcca atgtggttat cttatttgtc tccattattc ttagtgcatc
     4441 aacaatacgt tcattttctg taattgattt atagccaaca gtcataaaat catatgctgt
     4501 taaattagag atatcctgat atttctgaat agcccttctc acatctccat ctgtaataat
     4561 gccaataggt gt
//
LOCUS       DAOPUR010000051         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_19030_length_4563_cov_2.945209, whole genome shotgun sequence.
ACCESSION   DAOPUR010000051 DAOPUR010000000
VERSION     DAOPUR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19030_length_4563_cov_2.945209"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="PKN90_01430"
     CDS             <1..1505
                     /locus_tag="PKN90_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ84976.1"
                     /translation="MTAYKRVTNGEAFEKIAVEFSEDPTVQENKGRLGFITGMMVVYP
                     FEKTAYTTPVGQVSAPIRTRFGYHIIKVHDKRKTQGERLVAHILKRIPEDASEKQKAA
                     IERSIKSIYEDLAKGADFQRLAQANSDDQSSANKGGEISWVGTGKTTPEFENAVFNLQ
                     NIGDISQPVKTEFGWHIIKLLDKRDIMTLQEAYPLIEPRVKTDERASIIANSFIEKLK
                     KTYNFKSYPTNLHALQTLLAQPNDNILEQTKTYNLPIYTFADQVITQSDLIVFLHQQN
                     YTKNPIDLSLLDILANQVANNTIIKYENSMLEKKYPEFGLLMNEYKEGILLFNISNEM
                     VWSKASKDIEGLTDFFEKNKKSYAWDKPRFKGAIIYCDSKQSFKTAKKIAKATPEKEL
                     STKLKQTLNSNEKNAVKIETGLFAEQTNPVVDKLVFKKGKLEKNNDYPYIFTSGNIQK
                     HYPDSYQDIRGIVINDYQNYLDKQWIEELRSKYPIVIDTSVLNSLKSNNE"
     gene            1561..2361
                     /locus_tag="PKN90_01435"
     CDS             1561..2361
                     /locus_tag="PKN90_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84977.1"
                     /translation="MNITVDSDRVPVMSVNNEVLYADEIENILPANITGKDSVNFVEN
                     YKKNWAINILLYEKAEQNLRSTEEIDLLTEEYRKQLIINRYQQELISQKLKNISNDTL
                     INVYEREKANFILEDPIIKGVFVQIPSKAPNQKQLKKWLTEMNDNTLEKIEKYCIQYA
                     SRYIFFRDDWTPYSQVDKLFPHHVDWNDPILTRGLVEQKDSISTYFLRITGKANPGTI
                     EPFEIALPKITNILSQGEKMNYIKSFENSLYNNAIKKQKITFFLSSEE"
     gene            2373..3728
                     /locus_tag="PKN90_01440"
     CDS             2373..3728
                     /locus_tag="PKN90_01440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ84978.1"
                     /translation="MLKKITLLFVVCIFSIGVFSQNYIVDQVVWIVGDEAILQSEIET
                     EILRLKYEKQKTPESPECVIAEQLALQKLFLAQAKIDSITISEATINQQVESRIAYFT
                     EQLGSKEKVEAYFKKPLSKMREELKAMLQDQLMVQEMQQKITKDIKITPSEVTAFYEN
                     IPKDSLPIIPEQVEIQLITLSPTISVAEVERVKSKLREFRERIETGDIQFSTLAILYS
                     EDRVSALQGGEIGFRSRSMLVPEYANTAFSLTDPQKVSRIVETEYGFHIIQLIAKQDD
                     QVNSRHILMRPKISLEDKNLASQRLDSIAQAIRTGSINFEKGVEIYSDKSSTKNSNGL
                     LINTKDGSTRFQYDELPQEIINQVSFLNIGEISKPFSFTNENGKEIVAIVKIHDKIKA
                     HKASISDDYQTLKMVYTNVKNTQAIQQWIESKIATTYNQLTPAYRTCNFSYPHWLKNR
                     E"
     gene            3784..>4563
                     /locus_tag="PKN90_01445"
     CDS             3784..>4563
                     /locus_tag="PKN90_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNZ84979.1"
                     /translation="MKNNVSIIVFLYVMCIVLCNNANVTAQNKTTVFLENAETLSFDQ
                     NVRNNAQLLQGNVRFRHDKTLMLCDNAYFYEKTNSLDAFGNVKIIQGDTLFIYGNTLY
                     YNGNTRLAQMRNNVRMINRDAVLTTQKLDYDTYSNIGYYTDFGTLTDKKNVLTSKEGE
                     YLTKQNIIKFKKNVVLKNAEFTLYSDTLHYHTYNSLATIVGPTTIISDSTRIYAEKGW
                     YNTSTEDTKLTKNGKIQHKNGKSIQGDSIFYNKKYATIEAFS"
BASE COUNT         1810 a          810 c          703 g         1240 t
ORIGIN      
        1 caatgaccgc atacaaaaga gtaacaaatg gtgaagcatt cgaaaaaatc gctgtcgaat
       61 tttcagaaga ccctaccgta caagaaaaca aaggaaggtt aggcttcatt acaggcatga
      121 tggttgtcta tccttttgaa aaaacagcat atacaactcc tgtcggtcaa gtctcagcac
      181 caatcagaac aaggtttgga taccatatta ttaaagtaca cgacaaaaga aaaacacaag
      241 gagaacgatt ggttgcacat attttgaaac gtataccaga agatgcatca gaaaaacaaa
      301 aagcagctat tgaacgctca ataaagagta tatacgaaga tttagccaaa ggtgctgatt
      361 ttcaacgtct agctcaagca aactccgacg accagtcatc tgccaacaaa ggtggagaaa
      421 tttcttgggt aggaacagga aaaacaactc ccgaatttga aaatgctgtg tttaatctcc
      481 aaaatattgg agacatctct cagcccgtaa aaacagaatt tggctggcat attattaaat
      541 tgctcgacaa aagggatatt atgacattgc aagaagcata tcccctaatt gaacctcgcg
      601 tgaaaactga tgaacgagca agcattattg ccaatagctt tatagaaaaa cttaaaaaaa
      661 catacaactt taaaagttat cctacaaacc tacatgcatt acagactcta ctggcacaac
      721 caaacgataa catactcgaa caaacaaaaa catacaactt gcctatttat acatttgccg
      781 atcaggttat aactcaatca gacctaattg tgttcttaca ccaacaaaac tacacaaaaa
      841 atcctattga cctttctctc cttgatatat tggcaaatca agtagcaaat aacactatta
      901 tcaagtacga aaatagtatg ttagagaaaa aataccctga attcggactc ctcatgaatg
      961 aatataaaga aggtatttta ttatttaata ttagcaatga aatggtttgg tctaaagcct
     1021 caaaagatat agagggacta actgattttt ttgaaaagaa caaaaaaagt tatgcttggg
     1081 ataaaccccg ttttaaagga gctattattt actgcgactc aaaacaatct tttaaaactg
     1141 caaaaaaaat agctaaagca acccccgaaa aagaactatc aacaaaactt aaacaaacat
     1201 tgaattcaaa cgaaaaaaat gctgtaaaaa tcgaaacggg actttttgct gaacaaacaa
     1261 atccagtcgt agacaaactt gtatttaaaa aggggaaatt agaaaaaaat aacgattatc
     1321 cctatatatt taccagcggg aatatccaaa aacattatcc tgattcgtat caagatatac
     1381 gaggcattgt gataaatgat taccaaaatt atctcgataa acaatggata gaggaactac
     1441 gatcaaagta tcccatagta atagatacta gcgttttaaa tagtttaaag tctaataatg
     1501 aataaacgtt attccttcta tctcattctt atcacctcca cactattggg atgtaataag
     1561 gtgaacatta cagtcgactc agatcgggtg cctgttatgt ctgtaaataa tgaagtgtta
     1621 tatgcagatg aaatagaaaa tatattacct gcaaacataa caggaaaaga tagtgttaac
     1681 tttgtggaaa attataaaaa aaactgggca atcaatattt tattatacga aaaagctgaa
     1741 caaaacctac gttcaacaga agaaatagac ttgttaaccg aagaataccg aaaacaacta
     1801 attattaata gatatcagca agagcttatt tctcaaaaat taaaaaacat aagcaatgat
     1861 acattaataa acgtgtatga acgtgaaaaa gctaatttca ttttagaaga tccaattata
     1921 aaaggggtat ttgtacagat accaagcaaa gctccaaacc aaaaacaact aaaaaaatgg
     1981 ttgacagaaa tgaatgataa tactttagaa aaaatcgaaa aatactgtat tcaatatgct
     2041 tctcggtata tattttttcg agacgactgg actccatatt cacaagttga taagttattt
     2101 cctcatcatg tagactggaa tgacccaatt ttaactcgtg gtttagttga gcaaaaagac
     2161 agtatttcaa cgtatttttt gcgtattact ggaaaggcta accccggtac aattgaacca
     2221 tttgaaatcg ctctacctaa aatcacaaat attctctctc aaggagagaa aatgaattac
     2281 attaaaagtt ttgagaattc tctttacaat aacgcaatta aaaaacaaaa aatcaccttc
     2341 ttcctttcat ccgaagaata atagccacta ttatgttaaa aaaaatcacc cttctgtttg
     2401 ttgtatgtat attttcaata ggagtattct ctcaaaatta tatcgtagat caagtcgtat
     2461 ggattgtagg agatgaagcc atattacaat cagaaataga aaccgaaatt ctacgtttaa
     2521 aatatgaaaa acaaaaaact ccagaaagtc cagagtgtgt tattgctgag caactagccc
     2581 tacaaaaatt atttttagca caagctaaaa ttgatagtat tacgatctct gaggctacta
     2641 taaatcaaca ggtagaaagt cggattgcgt attttaccga acaactcggg tcaaaagaaa
     2701 aagtagaagc atattttaaa aaaccattat ctaaaatgcg agaagaacta aaagcaatgc
     2761 tccaagatca gctgatggta caagaaatgc aacaaaaaat tacgaaagat atcaaaatta
     2821 caccttcaga agtaaccgca ttttacgaga acattcccaa agatagttta ccaataatac
     2881 cagaacaagt agaaattcag cttattaccc tatctcctac tatatctgtt gcagaagttg
     2941 agcgagtaaa aagcaaatta cgcgaattcc gcgaacgaat tgaaacagga gatattcagt
     3001 tttctacctt ggctatttta tactccgaag atagggtgtc agccttacaa gggggcgaaa
     3061 ttggttttcg ttcgcgttca atgctagtgc ctgaatacgc aaacactgca ttttctctta
     3121 ctgaccctca aaaagtttct cgtatcgtcg aaacagagta tggttttcat attattcaac
     3181 ttatagcaaa acaggatgat caagtcaata gtagacacat actaatgcgt ccaaaaatat
     3241 cattggaaga taaaaatctt gcttcccaac gtcttgatag tatcgctcaa gcaatacgta
     3301 caggctctat aaatttcgaa aaaggagtgg aaatatattc tgataaatct tctacaaaaa
     3361 atagcaacgg attactcata aatactaagg atggtagcac tcggtttcaa tatgacgaat
     3421 taccacaaga aattataaat caagtttctt ttctaaacat cggggagatt tctaaaccat
     3481 ttagctttac caatgaaaac ggtaaagaaa tagttgctat agtaaaaata catgataaaa
     3541 ttaaggctca taaagcatct attagtgatg actatcaaac cttaaaaatg gtatacacga
     3601 atgtaaaaaa cacacaagca atccaacaat ggatagaaag taaaattgca acaacatata
     3661 atcagttgac ccctgcatat cgtacttgta acttttcata cccccattgg ctaaaaaaca
     3721 gagaataatc acaacaaatt gatgcaaaat gcacatatcc attggcagat taactacaag
     3781 aatatgaaaa acaatgtctc tattatcgtg tttttatatg ttatgtgtat tgtattgtgc
     3841 aacaacgcta acgtaactgc tcaaaataaa acgactgttt ttctagagaa tgctgaaacg
     3901 ctatcttttg atcaaaatgt acgaaataac gctcaacttc tacaaggaaa tgttcgattt
     3961 agacatgata aaacgttgat gctctgcgac aacgcatatt tttatgaaaa aaccaattca
     4021 ttggacgctt ttggtaatgt gaaaattata caaggagata ctctttttat ttatggaaat
     4081 actctttatt ataacggcaa tacacgtcta gcccaaatga gaaataacgt acgaatgatt
     4141 aaccgcgatg ccgtgctaac tacacaaaaa ctcgactacg acacatattc aaacattggc
     4201 tactacaccg attttggcac attaactgat aaaaaaaatg tattaacatc aaaagaaggc
     4261 gaatacttaa ccaaacaaaa cataatcaaa tttaaaaaaa atgttgtgct aaaaaacgct
     4321 gaatttacac tctactcgga cacattacac tatcacacat ataattcact tgctactatt
     4381 gttggaccca ccacaatcat ttctgatagc actcgtattt atgcagaaaa aggatggtat
     4441 aatacctcta ctgaagacac aaaattaact aaaaatggta aaattcagca taaaaacgga
     4501 aaaagcattc aaggagactc tatcttttat aacaaaaaat acgcgactat agaagctttt
     4561 tca
//
LOCUS       DAOPUR010000052         4548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_19132_length_4548_cov_3.193412, whole genome shotgun sequence.
ACCESSION   DAOPUR010000052 DAOPUR010000000
VERSION     DAOPUR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19132_length_4548_cov_3.193412"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKN90_01450"
     CDS             complement(<1..660)
                     /locus_tag="PKN90_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HNZ84980.1"
                     /translation="MEQDIKLLKGNEAVAEAAIRCGCDGYFGYPITPQSEIMETLMQL
                     EPWKTTGMVVLQAESEVAAINMVYGGAGCGKRVMTSSSSPGMSLKQEGLSYMAGADLP
                     GLVVSVMRGGPGLGTIQSSQADYFQTVKGGGHGDYKMITLAPASVQEMADFTVLGFEL
                     AFKYNIVSVVLADGIIGQMMEKVQLPPYQKRLTNDEIREKYPWATLGKPANRKPNVIT
                     SL"
     gene            complement(665..892)
                     /locus_tag="PKN90_01455"
     CDS             complement(665..892)
                     /locus_tag="PKN90_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ84981.1"
                     /translation="MAKIKGRVVIDNERCKGCDLCAVSCPTDVLAMSKSVNSRGYNFS
                     HAVNEEACIGCASCALVCPDACITVYKVKVE"
     gene            complement(990..1328)
                     /locus_tag="PKN90_01460"
     CDS             complement(990..1328)
                     /locus_tag="PKN90_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84982.1"
                     /translation="MKPIKNLEPFSKWILRLSVLCFLGTIYYKDLQKIDYQDYNYIIM
                     VVYILSSLLLVLGGLQKKATLTIISALFLTLISFYQIYLSFNGNFLQPSIYQHLMISG
                     IGLFFISKGN"
     gene            complement(1356..2672)
                     /locus_tag="PKN90_01465"
     CDS             complement(1356..2672)
                     /locus_tag="PKN90_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNZ84983.1"
                     /translation="MQHAPINPAIVQDVIDKLKLSDFSKATIREIVTIANAAETRTGK
                     KYIRMEMGVPGIDASEIGVKAEIKALEQGVAARYPMLEGIPQLKEESSRFIKAFVDVD
                     INPACCVPAIGSMNGTYASFLVAGQCHKEKDTVLFLDPGFPVQKMQLQVLGYKSVAFD
                     VYDYRGKKLQAKMEEFLKQGNISTIIYSNPNNPSWICLNQEELEIIGLLATKYDAIVL
                     EDLAYFAMDFRHDLGHPFEPPYQPSVVKYTDNYILLISGSKAFSYAGQRIGIIAMSPT
                     LYHRSYPHLIARYGVGEFGNILISRVLYSLSAGVTHSTQYALAAMFKAASDGSFHFLE
                     SVKEYGRRATLLKEIFARHHFSIVYDTDLKEPIADGFYFTVAYPHMKGCDLMKNLLFY
                     GISAISLNTTGSQQEGLRICTSFINDAQYDELDYRLSCFNKDFSIS"
     gene            2789..3868
                     /locus_tag="PKN90_01470"
     CDS             2789..3868
                     /locus_tag="PKN90_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNZ84984.1"
                     /translation="MKDKLVTLAIHTIEQALQLQHRLEKEGIPSFIKKIRSISTGVRV
                     RVREVDLQKAIYLVERIKSTSNEHIEFKSPFILVPIDFSEHAIHAAKLAFYLAENLHS
                     SIVFLHVETYVNVATFPTLLSSADEKSRLVTKKIKERNEAIIDVENRFKSLIANQLIP
                     NIHFNFELKNGIPEEEIISFAMLYKPLLIVMGTRSKANKEQELIGSVTAEVIERSLVP
                     VFVLPQHTHLQTIQQIKHIGFATNFDKKELVAFEKLMYFVQKLQFKVSFIHVDDKEDS
                     QNKNKLDELKSYFLTVYPHLQLQFSMLISEKSTLKELENYIRDQKIDILAIRNHKRGM
                     FVRLFAPSLARKLVYQAKNPMIVFY"
     gene            complement(3959..>4548)
                     /locus_tag="PKN90_01475"
     CDS             complement(3959..>4548)
                     /locus_tag="PKN90_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ84985.1"
                     /translation="PYSVVLFDEIEKAHPDVFNILLQVLDDGRLTDNKGRIVNFKNTI
                     IIMTSNIGSSLIRENFEHITASNRDNVIENTKNEIFELLKKSIKPEFLNRIDDLIMFT
                     PLSEEEIHQIVGLQIKLIANMLHKNDIDFSVTDKAIDLIAKIGYDPQFGARPIKRVLQ
                     RYVLNELSKQMIAENIDKSKPIIVDVAAGELIFKN"
BASE COUNT         1497 a          785 c          726 g         1540 t
ORIGIN      
        1 caaagaggta ataacattag gctttctatt ggcaggtttt cctaaagtag cccaaggata
       61 tttttctctg atttcatcgt ttgttaaacg tttttgatag ggtggaagct gtactttttc
      121 catcatttga ccaataatac catcagccaa caccacagat acaatattat atttaaatgc
      181 taattcaaac cctaatactg tgaaatcagc catttcttgt acagaggcag gagctaatgt
      241 tatcatttta tagtcaccat gaccaccacc tttaactgtt tgaaaataat cagcttgact
      301 agattgtata gttcctaaac caggaccacc gcgcattacg cttactacta atccaggtaa
      361 atcggcacca gccatatagg acagaccttc ttgttttagg ctcatacctg ggctagatga
      421 tgatgtcatt actctttttc cacagcctgc accaccgtat accatattaa tggcagccac
      481 ttcgctttcg gcttgtaaca ccaccattcc tgtagttttc caaggctcta gttgcattaa
      541 tgtttccata atttcagact ggggtgtaat agggtagcca aaatacccat cgcatccaca
      601 acgtatagcc gcttcggcaa cagcttcgtt gccttttaat aattttatgt cttgctccat
      661 gttgttattc tacttttact ttgtaaactg ttatacatgc atctggacac acgagtgcac
      721 aactggcaca accaatacat gcttcttcat taactgcatg agaaaagtta tatcctctgc
      781 tgtttacact tttagacatc gccaacacat ctgtggggca cgaaacagca cacaagtcgc
      841 atcctttaca acgctcattg tcgataacta ctctaccttt tatttttgcc attatagtga
      901 acaattttgg ttattttgaa agtacaaagt tattttattt ttcattcaat aagtttttaa
      961 gaacgttaaa gaatggtaac aacttgtatc tagtttcctt tagatataaa aaatagacca
     1021 attccactta tcattaggtg ttgataaata gagggttgca aaaaatttcc gttaaatgat
     1081 aggtaaattt ggtagaagga aataagtgtt aaaaatagag cacttattat tgtcagtgtt
     1141 gctttttttt gtaatccacc taatacaagt aacaaactac taagaatata tactaccata
     1201 atgatatagt tataatcttg gtagtcaatt ttttgtaaat ctttatagta gattgttcct
     1261 aaaaagcata ggacacttaa tcgcaatatc cactttgaaa aaggttctag gtttttaatt
     1321 ggtttcatag ttgatgaaat aaatgggtaa aatgattatg aaatggaaaa gtctttgtta
     1381 aaacaggata aacgatagtc taattcgtcg tattgagcat cattgataaa tgaggtacat
     1441 atacgtaatc cttcttgttg gcttcctgtt gtattgagag aaatggcact gataccataa
     1501 aaaagtaaat ttttcatcaa atcacaacct ttcatatgtg gatatgctac tgtgaaataa
     1561 aatccgtcag ctattggctc ttttaaatct gtatcataca caatagaaaa atggtgtcta
     1621 gcaaatattt ctttcagtaa tgttgctctt cgtccgtatt ctttaacgct ttctaaaaaa
     1681 tgaaaagagc catcacttgc tgccttaaac atggctgcta atgcatattg agtagagtgg
     1741 gttactccag ccgaaagaga atataaaacc ctacttataa gaatattacc aaattcacct
     1801 acaccatatc ttgctataag atgtggataa gaacgatggt atagggtcgg cgacatggca
     1861 ataataccga tacgttgacc tgcgtaacta aaagctttag agccagaaat taataaaata
     1921 taattatctg tatatttaac tactgatggt tgatatggtg gctcgaatgg atgccctagg
     1981 tcgtgcctaa aatccattgc aaaataggct aaatcttcta atacgatggc atcatattta
     2041 gttgctaata aaccaataat ttccaattct tcttgattta aacatatcca agatggattg
     2101 tttggatttg agtatataat ggtggaaata tttccttgtt tgagaaattc ttccatttta
     2161 gcctgtaatt ttttgcctct gtaatcatat acatcaaaag caactgattt ataaccaagc
     2221 acttgcaatt gcatcttttg tacaggaaaa cctgggtcga gaaacagtac agtatctttt
     2281 tctttgtggc actgaccagc aactaaaaag gaggcatatg tgccattcat agaaccaatt
     2341 gcaggaacac aacaagcagg attgatatca acatcaacaa aggctttgat gaaacgagaa
     2401 gattcctctt ttagttgtgg aataccttcc agcattggat agcgagctgc tacgccttgc
     2461 tctaatgctt taatttctgc tttaacacca atttcggagg catcaatacc aggtacaccc
     2521 atttccatac gtatgtactt ttttcccgta cgtgtttcgg cagcattggc gatagttaca
     2581 atttctcgta tagtagcctt actaaaatca gataacttta gcttatcaat aacatcttgc
     2641 actattgcag gattgatggg agcgtgttgc atacaaattt actctaagga ttatgttctt
     2701 ttttaatgct acaaatttag aaaaaatatt ttattttttg ctgttttaaa aaatatccat
     2761 tacttttact gactaactat tactatatat gaaagataaa ttagttacac tagcaattca
     2821 tacaatagaa caagcattac aattgcaaca tagactcgaa aaagaaggta ttccatcttt
     2881 tataaagaaa atacggagta tttctacggg agtacgtgta cgagtacgtg aagttgattt
     2941 gcaaaaagca atttatttag tagaaagaat caaatcaacc tcaaatgaac atattgaatt
     3001 taaatctcca tttattttag ttcccatcga ttttagtgaa catgctattc atgccgctaa
     3061 actagcattt tatttagcgg aaaacttaca ttcatctatc gtttttttgc atgtagaaac
     3121 atacgtaaat gtagcaactt ttccaacttt attatcctca gcagatgaaa aatcacggtt
     3181 ggtaacaaaa aaaattaaag aacgaaacga ggctataata gatgtggaaa atcgttttaa
     3241 atctttgatt gcaaatcaat taataccaaa tattcatttt aattttgaac ttaagaatgg
     3301 aattcctgaa gaagaaatca tctcttttgc catgctttat aagccgttac tcattgttat
     3361 gggaacacga agcaaagcta ataaagaaca agagcttatc gggagtgtaa cagctgaggt
     3421 aatagaacgt agtttggtac cagtttttgt attgccacag catacacatt tacaaactat
     3481 acaacagatc aaacatatag gatttgccac taattttgat aaaaaagagt tggttgcttt
     3541 tgaaaaattg atgtattttg tgcaaaaatt acaatttaaa gttagcttta ttcatgtaga
     3601 tgataaagaa gatagccaaa ataaaaataa attggatgaa ttaaaatcct attttcttac
     3661 tgtttatcct catttacaac ttcagttttc aatgcttata agtgaaaaat cgacccttaa
     3721 agaactagag aattatatcc gtgaccaaaa aatagatata ttggcaatac gcaatcataa
     3781 gagggggatg tttgtacgtt tgtttgcacc tagtttggct cgcaaactag tttatcaggc
     3841 gaaaaatccg atgattgtat tttattaatt aaattgtatc ataattaata ttatgttaaa
     3901 taggatgtat ccaaataaag cgacagttgt atttttgtcg ctttatttat tagtactatt
     3961 agtttttaaa aattagctcc cctgctgcta catctacaat aattggttta gatttatcaa
     4021 tattttcagc aatcatctgt ttcgacaatt cgttgagtac gtatcgttgt aatactcttt
     4081 ttattggtcg tgctccaaat tgtggatcgt aaccaatttt tgctattaaa tctatcgctt
     4141 tatctgttac agaaaaatct atatcatttt tgtgaagcat attggcaatt aattttattt
     4201 gtaagccaac aatctgatgt atttcctctt ctgacaatgg cgtaaacata attaaatcat
     4261 ctatacgatt caagaattcg ggtttgatac tcttttttaa tagctcaaaa atctcattct
     4321 tcgtattttc tatcacatta tcacgattag atgcagtaat atgttcaaaa ttctcacgta
     4381 ttaaggacga acctatattt gatgtcataa tgataattgt gttcttaaaa ttaacgatac
     4441 gtcctttgtt atcagtcaag cgaccatcat ctaatacttg caacaatatg ttaaatacat
     4501 caggatgtgc tttctcaatt tcgtcgaata acactacaga atatggct
//
LOCUS       DAOPUR010000053         4491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_19442_length_4491_cov_3.506087, whole genome shotgun sequence.
ACCESSION   DAOPUR010000053 DAOPUR010000000
VERSION     DAOPUR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19442_length_4491_cov_3.506087"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1208)
                     /locus_tag="PKN90_01480"
     CDS             complement(195..1208)
                     /locus_tag="PKN90_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84986.1"
                     /translation="MNLTDKKYANYALVQEMMETVKVVQKFNAQQTKNVASTIQSVGK
                     LFLTGEGSSRIFPAKNAIRKSLTKGLPVSIATDGSRQSAQYDLSKFAVFCASNSGRTK
                     EVVLLAKQLAAIGNVNRFALSANSGTLLEEVCTETFVLTCGWEQAVAATKSVVEQALF
                     YESILWHIEQTGTPNLADLAAKIEQALTFTIDSSIVQAAVKAPTIYFAGYNDGVAEEL
                     TLKTNEITRKKSDFLEGTYAVHGIEEVMEAQDVVFLIDPIEEEIQKFQEVLEQGVGLK
                     IIAIASRPTPFTTILVPDAGALSPYVYLCAGWNLLVEIGLALGIDLDKPERARKVGNE
                     LIV"
     gene            complement(1248..3452)
                     /locus_tag="PKN90_01485"
     CDS             complement(1248..3452)
                     /locus_tag="PKN90_01485"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNZ84987.1"
                     /translation="MPKLTILPSNSIGTNFIPTEFLSDNQNQFTFRNQQVNKPEDYWR
                     KLKSEEIERLVKNNNTATDWSKVLVTDEFDTDQILNNRFFGLVRIGSVRDVVIKHHDL
                     QLPVGITNSIVVSCDIGDDVAIHNVGYLSYYIIGDRCIISNVNELNTCNHAKFGNGIV
                     KEGEPEKIRVWLELMNETGCRGVAPFDGMITADAYMWARYRDDEALMKSLTQITQNSF
                     DTRRGYYGTIGDECVVKNSFVIKDVKVGTHCYIKGANKLKNLTINSSIEEPTQIGEGV
                     ELVNGVIGYGCHIFYGCKAVRFVMGNNSNLKYGARLINSFLGDNSTISCCEVLNNLIF
                     PAHEQHHNNSFLVAALVMGQSNLAAGATIGSNHNSRANDNEVQAGRGFWPGLCTSIKH
                     SCRFASFVMLSKADYPAEMYIPFPFSLLNNNVSKDQLEVTPAFWWMYNMYALERNTWK
                     FKGRDGRKRKVQHIELEALAPDSVEEIFDALHLLELWVGKAFYRKQQQNLSQLTDEAM
                     QQKGKELLFSTAEAVNALEVLGEGMEKTKRKVLIIKAYQSYHAYRDMVYYYAVNTMLQ
                     WMQHDTSRTFADMVVALKGDRQTKWSNFGGQLMREQDVDALRQDIGNGKLTTWNQIHQ
                     RYDSLWEQYPLHKQQHAYASLCSVLGVSNLTQKQWLEVLDKSLEIQEFVKDQVYLSRK
                     KDFDNPYRRMTFRNEAEMTAAIGTIEDNGFVKEVRQMTVKFNDLVKEIKQRG"
     gene            complement(3753..4157)
                     /locus_tag="PKN90_01490"
     CDS             complement(3753..4157)
                     /locus_tag="PKN90_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNZ84988.1"
                     /translation="MNPIDILKTHQLKRTVCREGILSALLSANKALSEEEVKSKIDGN
                     FDRTTFYRSFKTLIEHNVLHKIVVDEGLVKYAITIVGKTPEHHHAHFVCKKCHTVSCL
                     DAFTFKVPNLPSGYQTEDSDLLIKGVCKMCTI"
BASE COUNT         1362 a          903 c          712 g         1514 t
ORIGIN      
        1 tgcattaagt atggaggtaa tgggctttat acactattat ttgggaattc ataaatcata
       61 agtcttttta tatcatcaga ataattaaca agcttaaacc gattttttgt ttatagaata
      121 aagcttaccc tcaaaaaaca aaaaaataga ggtattacgt atcgacatac ccctatttca
      181 gtatataaca tatcttacac aatcaactca tttcctactt tacgagcacg ttctggttta
      241 tccaaatcaa tgcctaaagc aagcccaatt tctaccaata aattccaacc agcacacaaa
      301 taaacataag gagataaggc tcctgcatct ggtactaaaa tggtagtaaa aggcgtaggt
      361 cttgatgcaa ttgcaatgat ttttaagcct actccttgtt ctaaaacttc ttgaaatttt
      421 tgaatttcct cttctattgg atcaatcaaa aataccacat cttgagcctc cattacctct
      481 tcaataccgt gtacagcata ggtaccttca aggaagtcgg attttttacg tgtaatttca
      541 ttggttttta atgttagttc ttcggctaca ccatcgttat aaccagcaaa atagatagta
      601 ggtgctttta ccgcagcttg cacaatagat gaatcaattg taaacgttaa tgcttgttca
      661 atttttgcag ctaaatcagc taagtttgga gtacctgttt gttcgatatg ccacaaaatt
      721 gactcataaa acaacgcttg ttctactaca cttttggttg cagctacagc ttgttcccag
      781 ccacaagtaa gtacaaatgt ttcggtacaa acttcttcta acaatgttcc gctatttgca
      841 cttaatgcaa aacggttgac attaccaata gcagctaatt gttttgccaa caatacaacc
      901 tctttggttc tgcccgaatt agaggcacaa aacacagcaa acttactcaa atcatattgt
      961 gccgactgac gagaaccatc ggtagctatg cttactggca atccttttgt tagagattta
     1021 cgaatggcat ttttggctgg aaaaattctg ctagaacctt cgcctgttaa aaataatttg
     1081 cctactgatt gaatggttga tgccacattt tttgtttgtt gggcattaaa tttttgtact
     1141 accttcactg tctccatcat ttcttgaacc agtgcgtagt ttgcgtactt tttgtctgtt
     1201 aaattcattt ttttgcgttt ttttaacgta taaataccta gagatgatta acctctttgt
     1261 ttgatttctt ttactaaatc attaaatttt actgtcattt gacgtacctc ctttacaaaa
     1321 ccattgtcct cgatagttcc aatagcagcg gtcatttcgg cttcgtttct aaatgtcatt
     1381 ctgcgatatg gattatcaaa atcctttttg cgcgacaagt acacttggtc ttttacaaac
     1441 tcttgtattt caagcgattt gtctaatact tctagccatt gcttttgagt taggttactc
     1501 acgcctaaca cagagcacaa cgaagcatat gcgtgttgct gtttgtgtaa tgggtattgc
     1561 tcccacaaac tatcgtatcg ttggtgtatt tgattccaag ttgttagttt gccattccct
     1621 atatcttgac gcaaagcgtc tacatcttgt tcacgcatca attgaccacc aaaatttgac
     1681 catttcgttt gtctatctcc ttttaaagca actaccatat cagcaaaggt gcgcgatgta
     1741 tcgtgctgca tccactgcaa catcgtgttt actgcataat aatacaccat atccctatag
     1801 gcatgatacg attgatacgc tttgatgata agcacttttc gctttgtttt ctccattcct
     1861 tcgcctaaaa cctctaaggc atttacagct tcggcagtcg aaaacaataa ttcttttcct
     1921 ttttgttgca ttgcctcatc ggtaagttgg ctaaggtttt gttgttgctt acggtaaaac
     1981 gcttttccta cccacagttc aagtaagtgc agggcatcaa aaatctcctc aaccgaatca
     2041 ggagctaagg cttctaattc gatgtgttgc acttttcttt tacgaccatc tctgccttta
     2101 aatttccaag tattgcgttc tagggcatac atgttataca tccaccaaaa agcaggagta
     2161 acttctaatt ggtctttaga aacattattg ttgagcaacg aaaatgggaa aggaatatac
     2221 atttcggcgg ggtaatcggc tttggataac attacgaaag aggcaaaacg acaagaatgc
     2281 ttaatacttg tgcataatcc gggccaaaac cccctacccg cttgtacttc gttatcatta
     2341 gccctgctat tgtgattgga gccaatggtt gcccctgcag ccaaattgct ttgtcccata
     2401 accaaggcag ctaccaaaaa ggagttgtta tgatgctgtt cgtgtgctgg gaatataaga
     2461 ttattgagca cttcacaaca cgatattgtt gaattatctc ctaagaacga attaattagt
     2521 cgggctccat actttaaatt tgagttattt cccataacaa aacgcaccgc tttgcatcca
     2581 taaaaaatat gacagccata cccgataaca ccgtttacta gttccacccc ttcgccaatt
     2641 tgtgttggtt cttcaataga cgagttgatg gttagatttt taagcttatt tgccccttta
     2701 atataacaat gagttcctac ttttacatct ttaattacaa aagaattttt taccacacat
     2761 tcatctccaa ttgtaccata ataaccacgt cttgtgtcaa aactattttg agtaatttga
     2821 gtgagtgatt tcattagcgc ctcatcatct cggtagcgtg cccacatgta tgcatcggca
     2881 gtaatcatac catcaaaggg agctactcct ctgcacccag tttcgttcat taattcgagc
     2941 caaacacgta ttttttcggg ttctccttct tttactattc cattaccaaa ttttgcatgg
     3001 ttgcatgtat ttagttcgtt tacattagaa ataatgcacc tatcgccaat aatatagtac
     3061 gataaataac ctacattatg aatagctaca tcatcgccta tatcacacga tactacgata
     3121 ctattggtaa tacctacggg caactgtaaa tcgtggtgtt ttattaccac gtcgcgtaca
     3181 gatccaatac gaaccaaccc aaaaaaacgg ttatttaaaa tttgatcggt atcaaactca
     3241 tccgtaacca acacctttga ccagtcggtt gcagtattgt tattttttac taacctttcg
     3301 atttcctccg attttagttt tctccaataa tcctcaggtt tattaacttg ttgattgcga
     3361 aaggtaaatt gattttgatt gtctgataaa aattctgttg gaataaaatt agtaccaata
     3421 gaattcgaag gtaaaatcgt taacttgggc ataaagtaga aatttgttat ataaaattta
     3481 ttatctcaca taatacgaat aagaaagccc gtaaaactac taaaaatttg atataaatct
     3541 agcattttgt aaataaaatt cgtatcaacc atatctgcta ttcttctgac ttatgaaata
     3601 tttttatgga aatacctttt tgggtcgacc atccttgttt atcggtaaga gatataaata
     3661 aacaaagaga tataaatagt ttcattcgat agtatccttt ttgataaggg tagtcatagt
     3721 ttactaaaaa cggataatct gtttttgcaa aactatatag tacacatttt gcaaacgccc
     3781 ttaatgagta aatcagaatc ttctgtttgg taaccagagg gcaaattggg cactttaaac
     3841 gtaaacgcat ctaaacaact aacggtatga catttcttac acacaaaatg agcatgatga
     3901 tgctcggggg ttttgcctac aatggtaatg gcatatttca ccaagccctc atctaccaca
     3961 atcttgtgca acacattatg ctcaataagc gttttaaacg agcgataaaa tgtagtccga
     4021 tcaaaatttc catcaatttt gcttttcact tcttcttccg acaatgcttt gttggctgac
     4081 aatagtgcag ataggattcc ttctctacaa acagttcgtt taagctggtg tgtctttaaa
     4141 atatcaattg gattcataaa ataaacttat tttgcgtgaa tagttaattt tgcaacaatg
     4201 ttctatttgc atcattgttg caaatacata gctaaaaata gtttttccta tgtatactat
     4261 taactatttt tattttatta ccaaatttgc ataacctaaa acaaatactt acttttgcag
     4321 cgacaaaatt tacagttaat cagtttttta cacaattcga taacaagata acagataact
     4381 aataaaaagc actatgaaga aaacagcttt tttactagca tcactcgttg gatgcctatt
     4441 ttatgctcag gcacaagaca atgttagtgc tgagaaaccg ctaattcccg t
//
LOCUS       DAOPUR010000054         4486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_19467_length_4486_cov_3.224554, whole genome shotgun sequence.
ACCESSION   DAOPUR010000054 DAOPUR010000000
VERSION     DAOPUR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19467_length_4486_cov_3.224554"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKN90_01495"
     CDS             <1..362
                     /locus_tag="PKN90_01495"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNZ84989.1"
                     /translation="PTNTSLFSILQAQDPQNAIIRSYGLYNLYYQYHNKPNIGTHKHA
                     TEWGVLDQMIVSGNALQPDAFFYTSEQSAKIFDAEFLLIDDETYFGKKTFRTYLGMKY
                     IGGFADHLPIYIDFFQR"
     gene            complement(372..914)
                     /gene="rsmD"
                     /locus_tag="PKN90_01500"
     CDS             complement(372..914)
                     /gene="rsmD"
                     /locus_tag="PKN90_01500"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNZ84990.1"
                     /translation="MRIISGIYKGRRFTPPASLKARPTTDFAKEGLFNVLANEIDIEE
                     AQVLDLFAGSGSISFEFVSRGAGHTTSIEQSFQHGAFIKKTAQELNIKTLSVLKADVF
                     RYIETCNHSYDIIFADPPYSLPQLAEIPNKIMNSALLKPDGLFILEHGKNNTFGEHPN
                     FINTRHYGNVHFSFFKHKTK"
     gene            complement(905..1753)
                     /locus_tag="PKN90_01505"
     CDS             complement(905..1753)
                     /locus_tag="PKN90_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNZ84991.1"
                     /translation="MMHLLVKDTAFDSKNTREYDLSIRISADGFSFCIYHPLTKTFVC
                     CKHVDFSVKDTKYYRNQAIALCEPYLAYEYRRTLVVFADEKSTLIPEGVFQPADYLNI
                     LHFNYPETKHSGYELLINNLTVFSITNIFAVYTEQITFWKEVFPSAEFIHMHTPLIDA
                     LLKESKKEDIQLVWLYIQPTSIFLSYADKGKLIFSNTFPIQSATDIVYACGNVFEQFG
                     LSQHHTKLILTGNYTSKSEIYSLLHTHFAMLDEYSLASDIIIQSSCKSSDIQKLMDLL
                     LVPLCV"
     gene            complement(1762..2397)
                     /locus_tag="PKN90_01510"
     CDS             complement(1762..2397)
                     /locus_tag="PKN90_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84992.1"
                     /translation="MDKIFKLILVAVSIVLGYLCVQSILIPIKFNEEKSFREKVVIAR
                     LADIRRAQVEYRTAKGVYTNSFDTLIAFVRQGKAPIILKEGSLTDKQLEAGLTEKEAV
                     RQGIIKRDTTYVSILESLFGKNYPIDSIRYVPFSNPLTEFEMEKGELTSGAAAISVKV
                     VEVRTPYTVYLNGLNRQEIINIVETQEKMNKYPGLKFGSVTEANNNAGNWE"
     gene            2577..3974
                     /locus_tag="PKN90_01515"
                     /pseudo
     CDS             2577..3974
                     /locus_tag="PKN90_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244863.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            4056..>4486
                     /locus_tag="PKN90_01520"
     CDS             4056..>4486
                     /locus_tag="PKN90_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84993.1"
                     /translation="MKNRILHQLLFLVFSLFFLLTSCIDDSYKIDDIDTTVGLDMALV
                     GPIGKSKITLSSVFPDDLENYELLIRNDSLFIVLHDTVDFGSTFIDDIKIPKGQYTKH
                     IPVIDSDLLPTTSVDVDVTLDYEFDINTNPNERIDSLVFNTS"
BASE COUNT         1434 a          681 c          822 g         1549 t
ORIGIN      
        1 atcccaccaa cacaagttta ttttctatat tgcaagccca agatccacaa aatgcaatta
       61 ttcgttcgta cggtttgtat aacttatatt atcagtatca taacaaaccg aatataggta
      121 ctcataagca tgcaacagaa tggggggtat tagaccaaat gattgtttca ggcaatgcat
      181 tacaaccaga tgcgtttttt tatacatcag agcagtcagc aaaaatcttt gatgctgaat
      241 ttttattgat agatgatgaa acctattttg gtaaaaaaac ctttcgaacg tacttgggga
      301 tgaaatatat tggagggttt gctgatcatt tacctattta tatagatttt tttcaacgtt
      361 aagtttttgg tctatttcgt tttatgttta aaaaaggaaa aatgaacatt cccataatgt
      421 ctggtattaa taaagttggg atgttcgcca aaagtattat ttttaccatg ctctaaaata
      481 aacagcccat ctggcttcaa tagtgctgaa ttcataattt tgtttggaat ttcagctaat
      541 tgaggcaggc tatagggtgg gtctgcaaaa ataatatcat acgagtgatt gcaggtttct
      601 atgtatcgaa aaacatctgc tttcagtaca gatagtgttt taatattgag ttcttgagcc
      661 gtttttttta taaatgcacc atgctgaaag ctctgttcta tacttgttgt gtgtcctgct
      721 cctcttgata caaactcaaa agaaatactt ccacttccag caaatagatc tagcacctgt
      781 gcttcttcaa tgtctatttc attagctaac acattaaata atccttcttt cgcaaaatct
      841 gttgttggtc gtgctttgag tgatgcaggt ggagtaaatc ttctgccttt gtatatgcca
      901 cttattatac gcataatggt acaagtaata gatccataag tttctgtatg tcagatgatt
      961 tgcaagaaga ttgaataatt atatctgatg caagagaata ttcatcaagc atagcaaaat
     1021 gtgtgtgaag taaggagtat atttcgcttt tggatgtata attaccagta agaataagtt
     1081 tggtgtggtg ttggcttagc ccaaattgtt caaatacatt gccacatgca tatacaatat
     1141 ctgtggcaga ttgaataggg aatgtgttag aaaaaatgag tttccccttg tctgcatagg
     1201 ataaaaaaat acttgttggt tgaatatata accatacgag ttgaatgtct tcttttttag
     1261 attctttaag caatgcatca atgagaggag tatgcatatg tatgaattct gcagagggga
     1321 acacttcttt ccaaaatgta atttgttcgg tgtatactgc aaatatattg gtaatagaaa
     1381 atacggttaa gttattgatg agcaattcgt agcctgaatg ttttgtttca ggataattaa
     1441 aatgtaaaat atttagataa tcagcaggtt gaaatactcc ttctggtata agtgtgcttt
     1501 tttcgtcagc aaatacaacc aaggtacgac ggtattcgta cgctaaatat ggttcgcaca
     1561 aagcaatagc ttgattgcgg taatattttg tgtctttcac cgaaaaatct acatgcttac
     1621 aacatacaaa agttttcgtt aggggatgat aaatgcaaaa tgaaaatcca tccgctgata
     1681 tgcgaatgga taagtcatat tcacgggtat ttttagaatc aaaagcggta tctttaacca
     1741 ataagtgcat catttatgtt tctattccca gttgcctgca ttattgttgg cttctgttac
     1801 tgacccaaat tttaatccag gatatttatt cattttttct tgcgtttcca caatatttat
     1861 gatttcttgt ctatttaatc catttaaata aacagtgtat ggagttctta cctctactac
     1921 ttttacactt atagcggctg ctccagaagt taattcccct ttttccattt caaactcagt
     1981 taaagggtta gaaaatggga cataacgaat agaatcaatg ggataatttt tgccaaataa
     2041 cgattctaaa atacttacat atgttgtatc tcttttgatg attccttgac gaacagcttc
     2101 tttttcagtc agacctgctt ccaattgttt gtccgttaaa cttccttctt taagaataat
     2161 aggagctttc ccttgtctaa caaaagcaat aagtgtatcg aaactatttg tatatacgcc
     2221 tttcgctgtg cgatattcaa cttgtgctct tctgatatct gctaaacgtg ctataactac
     2281 cttttcacga aaagattttt cttcgttaaa tttgatagga attaaaatac tttgtacgca
     2341 taaatagcct aatacaatac ttacagcaac taaaattagt ttaaaaattt tgtccattgg
     2401 tgttacaagg ttttgttttt agaatgacgc aaagttaaga aagaatttgg aactaattat
     2461 ctttatttga aaaaaaaata gatgtgtaga gctaattttt ttttaattca ttacttttgt
     2521 gtcaaactaa atatttcacg tatatcttat ttttactcgt taatataagt ctgataatgc
     2581 ttacacgata tatagaaaat aaaaccacag gctatttagg atttactcct acgccagaac
     2641 aagcagagtt gatatcccaa cttgctgttt ttttggctga accgcaaaaa cgccatacct
     2701 ttttacttaa aggatatgca ggaacgggta aaacatctgt ggtaagtgct attgttaaat
     2761 tgttagatga attgcaacag cccgttattt tattggcacc aacaggaaga gccgccaaag
     2821 tcttgtcttc gtattctggc aaacaagcag caaccattca taaacatatt tatcgcaaaa
     2881 aaacagccat agctgatgca ccttttgata tagcataaaa tagttataaa aatgcattat
     2941 tcattgtaga tgaagcatct atggtggcaa atacaggaga tggtctgttt ggaagtggtc
     3001 gtttactgga tgatttgatt acctacgtgt ttagtgctga gaattgccat ttattattgt
     3061 tgggtgatac tgcccagtta ccacccgtaa tgcaagaaga aagtgaggca ttggacacta
     3121 aaatccttga aaaatatgat ttatatgtgc aagaatatac ccttacacaa gtggtacgtc
     3181 aggcagaagc aagtggaatt ttacgcaacg ccacacatat ccgacaaatg ataagtaatc
     3241 agataaatat acacagtttt attttacaca cagatagtga tgtgatacgt gtgtcaggtc
     3301 aagattttgt agaaactttg tatacagcat atcgtgatgg agtagacgaa accattctta
     3361 ttacacgttc taacaaaaaa gcggctatgt atgtggaggg aattcgtgct aggatattac
     3421 aaagagaaga gttgttatct gcaggagatt tggtaatggt ggtgaaaaat aattattttg
     3481 ttggcaagga gtatcagggg tttgaattta ttgcaaacgg agatatagca gaggttttgc
     3541 gtgtacgttc ctattcacaa ttatacggat ttacttttgc tgatgtagtt gttcggtttt
     3601 tagactacga ttgggaggtg gatgccaaaa ttttggtaga tgtcttgtct tgcgttactc
     3661 ctgccgaatt acaggaaatg aataaaaaat tatttaaaac agttgaggag gattatgcac
     3721 atattggcaa taaaagaact aggtataaag aaatgaaaaa tgatatttat ttgaatgcac
     3781 ttcagctaaa gtttgcttat gcaattactt gtcataaagc acaaggagga caatggaaac
     3841 atgtttttgt ggatatggga atgattaaaa aaacagaaat tgatacctca tttttacgtt
     3901 ggttgtatac tgctagtact cgagctaccc aaaaattgta tttaataaat tttgatgatg
     3961 catttttttg ttaaaaattg tttcgattta tcaaattttg tactatcttc gtacaagtaa
     4021 tttagaaaga aaaatgagcc attaaattta tgcgtatgaa aaatagaata ctacaccaac
     4081 ttttattcct agttttttct ttattttttt tattaacaag ctgtatagat gatagttata
     4141 aaattgatga tatagatact acggtaggac tggatatggc gttagttggt cctataggta
     4201 aatctaaaat tacgctttcg tctgtttttc ctgatgatct tgaaaattac gaattattga
     4261 ttcgtaacga cagtcttttt attgtattac acgatactgt tgattttgga agtactttta
     4321 ttgatgatat taaaatacct aaagggcagt atacaaagca tatccctgtg attgatagcg
     4381 atttgttgcc taccacgagt gttgatgttg atgtaacgtt ggactatgaa tttgatatca
     4441 ataccaatcc gaatgagcgg atagatagtc ttgttttcaa tacgtc
//
LOCUS       DAOPUR010000055         4426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_19853_length_4426_cov_3.617708, whole genome shotgun sequence.
ACCESSION   DAOPUR010000055 DAOPUR010000000
VERSION     DAOPUR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19853_length_4426_cov_3.617708"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKN90_01525"
     CDS             complement(<1..330)
                     /locus_tag="PKN90_01525"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNZ84994.1"
                     /translation="MSFIADKIAFDGLTFDDVLLIPAFSEVLPREVNLSTKFSRNIEL
                     KIPFVTAAMDTVTEAKMAIAIAREGGIGVIHKNMSIEEQARQVAIVKRAENGMIYDPV
                     TIKRGSTV"
     gene            complement(456..1367)
                     /locus_tag="PKN90_01530"
     CDS             complement(456..1367)
                     /locus_tag="PKN90_01530"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNZ84995.1"
                     /translation="MKAELTILGCGSAMPSYGRFSASQVLSVRDKSFMIDCADGTLFR
                     MREIAIKTNRLNHIFISHLHGDHCFGLMAVLSTLGLMKRTADIYIHAHPDLENLLQPH
                     LRYFCNDLTYKIFFVPYNPLQSEIIYEDKSLTVTTIPLRHGIPTCGFLFKEKPQQLHI
                     KRDMIDFYQVGIKDLNAIKDGKDYITNQGETIPNSFFTTPASPQKSYAYCCDTAYSEK
                     IIPIIEGVDCLYHDATFAENEVLRAKKTLHSTAKQAATIASKAQVKQLLLGHFSARYP
                     HVSVILNEAQTIFANSIIAEDKKTYTF"
     gene            complement(1364..3268)
                     /locus_tag="PKN90_01535"
     CDS             complement(1364..3268)
                     /locus_tag="PKN90_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNZ84996.1"
                     /translation="MYHEILHTYWGYSEFRSLQLDIIQSIGNGKDTLGLMPTGGGKSL
                     TFQVPALAKEGLCLVITPLIALMKDQVENLKKRGIKATAIYSGMSYEEMMIAIDNCMY
                     GNYKFLYVSPERLSTRAFLLKIPHLQINLIAVDESHCISQWGYDFRPSYRQIASIREH
                     LPTVPILALTATATPEVVRDIQTQLKFAAPNVFQKSFVRSNLIYIVRQTDDKLQQLLQ
                     ILQKVPGTSVVYVRNRKKTKEIADFLNENNIPANYFHAGLSNNTKDERQQKWKNDECR
                     VMVSTNAFGMGIDKPEVRTVIHLDLPDSIENYFQEAGRAGRDEKKAYAILLYSNADKV
                     KIEKRIRDNFPEKEKIKEIYEAISNYLQIPVEGKEGAVYDFEIFDFCAKFHFPALLVH
                     SAINILEQAEYICYTEEVDSNSRILFTVSRDDLYAINNSHIHSEKIIHALLRSYTGLF
                     TDYAYINEEIIAQRAGLTVDETIQTLIFLAKTKVIQYIPRKKTPFITYTQQRQEKRYL
                     KISREVYEEKKERYVKRIESMIAYATNTTVCRSRMLLHYFGESNTHDCDQCDICLTRK
                     KNDLANQEFQQLCTTIKTTLLEKALTIHELSAKYNHQKEKIIKTTRWMLDQGEIYCDD
                     TLKLHLNKTK"
     gene            complement(3289..4386)
                     /locus_tag="PKN90_01540"
                     /pseudo
     CDS             complement(3289..4386)
                     /locus_tag="PKN90_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
BASE COUNT         1268 a          738 c          814 g         1606 t
ORIGIN      
        1 cacagtagat cctcgtttaa tggtaacggg gtcgtaaatc atgccgtttt cggcacgttt
       61 aacaatggct acttgtcgag cttgttcctc tattgacatg tttttgtgga taacaccaat
      121 acctccttct cgagcaattg caatagccat ttttgcctcg gttacggtat ccatggcagc
      181 tgttacaaaa ggaattttta attcaatatt tctagaaaac tttgtagaaa gatttacttc
      241 gcgtggaaga acttcggaaa atgcggggat gaggagtaca tcatcaaatg ttaacccatc
      301 aaaagctatt ttatctgcaa taaacgacat acgatttagg tattaaaatt attgcgtgca
      361 aatatacgaa aaaaatatcg aacttacggt attctatcta cgttaaaatt agttagctaa
      421 aggcgtattt ttggatagta agtgttattt tttgtctaaa aagtatatgt ttttttatct
      481 tcggctatta tactattggc aaatattgtt tgagcttcgt ttaaaataac ggatacatgt
      541 ggatagcgag ctgagaaatg tcctaatagt aattgtttta cttgtgcttt tgaggcaatt
      601 gttgcggctt gttttgctgt agaatgtaat gttttttttg ctctgagtac ttcattttcg
      661 gcaaaagtag catcatgata caaacaatct accccttcta ttatgggtat tattttttca
      721 gaatatgctg tatcacaaca ataagcatag cttttttgtg ggctggctgg agttgtaaaa
      781 aaggagtttg gtattgtttc tccttggttt gttatgtaat cttttccgtc tttaatggca
      841 ttcaaatctt ttattcctac ttggtaaaaa tcaatcatat ctcgtttgat atggagttgt
      901 tgaggttttt ctttgaaaag aaatccacaa gtaggtatgc catggcgtaa tgggatggtt
      961 gttactgtta gtgatttatc ttcgtatatt atttcgcttt gtagcggatt gtagggtaca
     1021 aaaaagattt tgtatgttaa atcgttacaa aaatagcgta agtggggttg tagcaaattt
     1081 tccaaatcag ggtgggcatg aatatatata tcagcggttc ttttcatcaa gcctaaggta
     1141 gataatacag ccataagccc aaaacagtgg tctccatgta aatgcgaaat aaatatgtga
     1201 ttgagacgat ttgttttgat agctatttct ctcattctaa agagagtgcc atcagcacaa
     1261 tcaatcatga aacttttatc gcgtacagat aacacctgtg aagcggaaaa tctgccatac
     1321 gatggcatag cagatccgca tcctaatatg gttaattcag ctttcatttt gttttattaa
     1381 gatgtaattt caacgtatca tcacaatata tctctccttg gtcaagcatc catcgtgtag
     1441 ttttaatgat tttttctttt tggtggttgt attttgctga caattcgtgt atggttagtg
     1501 ctttttctag taaagttgtt tttatggtag tgcaaagttg ttgaaattct tggttggcta
     1561 aatcattttt tttacgtgtc aagcaaatat cacactgatc acaatcatgt gtgttgcttt
     1621 cgccaaaata atgcaatagc attcggcttc ggcaaacggt agtatttgta gcgtaggcaa
     1681 tcattgattc gatacgcttt acataacgtt cttttttttc ttcgtatact tctctcgaaa
     1741 tttttagata tcgtttttct tgtcgttgtt gggtgtaggt aataaaaggt gttttttttc
     1801 gaggaatata ttgaataact ttagttttag ctaaaaagat tagtgtctga attgtttcgt
     1861 ctactgttaa tccagcacgt tgtgcaatta tctcttcgtt gatgtatgca taatccgtaa
     1921 atagtcctgt gtatgagcgt aaaagagcat ggattatttt ttctgaatgt atatgggagt
     1981 tgttaattgc atacaaatca tcacgcgaaa cagtaaatag aatgcgcgaa ttactgtcta
     2041 cttcttctgt atagcatata tattctgctt gttctagaat attgattgca ctatgaacaa
     2101 gtaaagctgg aaagtggaat ttggcacaaa aatcaaatat ctcaaaatca tatacagctc
     2161 cctcttttcc ttctacgggt atttgcaggt aattggatat ggcttcgtaa atttctttaa
     2221 ttttttcttt ttcagggaag ttgtctcgta ttcttttttc aatttttact ttatcagcat
     2281 tactatacag tagtatggca tacgcctttt tttcgtctct accagctcta ccagcttctt
     2341 gaaaatagtt ttcaatgcta tctggtaagt ctaaatggat aacagtacgt acctctggtt
     2401 tatctatgcc cataccaaat gcgttggtag ataccattac acgacattca tcatttttcc
     2461 atttttgttg gcgttcatct ttggtattat tactaagtcc tgcgtggaaa taattagcag
     2521 ggatattgtt ttcgtttaaa aaatcagcta tttcttttgt tttttttctg tttcttacat
     2581 atacaacaga agtaccaggc actttttgca gtatttgtag taattgttgc agtttatcat
     2641 cggtttgacg tacaatataa attaaattgc tccgaacaaa acttttttga aacacatttg
     2701 gtgcagcaaa tttgagttgt gtttgaatgt cgcgtactac ttcgggggtt gctgtagccg
     2761 taagagctaa aataggtact gtaggtaaat gttctctaat agatgctatt tgacgatatg
     2821 aaggacgaaa atcgtatccc cattgtgaaa tgcaatgaga ttcgtcaacg gcaataagat
     2881 tgatttgtag atgaggtatt ttaagtaaaa aagctcgagt tgaaagtcgt tcgggagaaa
     2941 cgtataaaaa cttataattc ccatacatgc aattatctat agcaatcatc atttcttcat
     3001 agctcatgcc actgtatatg gcagttgctt tgattcctct tttcttcagg ttttctactt
     3061 ggtctttcat caaagcaata agaggagtaa tgaccaaaca caaaccttct tttgctagag
     3121 ctggtacttg aaaagtaagc gatttacctc caccagttgg cattaagccc aaggtgtctt
     3181 tgccgtttcc aatagactga ataatatcaa gttgtaatga acggaattca gaatatcccc
     3241 aatatgtatg taaaatttcg tggtacatgt taggttatta actgctattt attcattaat
     3301 tttcatatcc tttatcatta gttggatggt tttatttcca ttaaagacgt tttcttccaa
     3361 cgtataacaa atatcgattg gtttgaggtc tttaatgtga gcactaaatt tttctcccat
     3421 cccaaaagca attccattca ttacattttc tgagttgggc tcagttagtt ctagttttag
     3481 atgttctaat tcttttccta ctagtcggct ggagccaaaa tctgtaactt tatgggttgc
     3541 aaagattggt ttcatgtttt caggaccgta tggtgcaaat tgttgtaata tattaaaaaa
     3601 ctttggcgtt atatcactaa aacggataat ggcgtcaata tctatttgag gttgtgtttg
     3661 ctcttcggta atattttcgc ggacatagtt atcaaaacgt tctgtaaaaa ttgacacatt
     3721 gtcttctttc atggtcaatc cagctgcata catatgtcct ccaaagtttt ctaacaaatc
     3781 ccgacacgat tctattgctt tataaatatc aaaaccagta acagaacgtg cagaaccagt
     3841 agcaaaacca tttgaaagcg taagaacaat agtaggtttg tggtataatt cgctcagcct
     3901 agatgccaca ataccaataa ctcctttact ccattcagga tgataaacga cgatggagtt
     3961 tctgtgttca aaatcttcgt tcccttggat gaagtcttta gcgtcatctg ttgttttttt
     4021 gtccaaatct ttgcgttctt gattgtattg gtctatgcag tcgcttttag ccttagcaaa
     4081 gcttagattt ttactaacta ataactctac tgcttcgttg ccagatctca ttctgcctga
     4141 tgcattaata cgtggaccta ttttaaaaac gatatcagaa gtagtgattt ctttgcctct
     4201 caagccacat acatcaataa tggcttgtag cccaacacat ggattcgcgt ttagttgttt
     4261 taagccaaaa aacatcaaga ttctattttc gcctgtaatg gggacaatgt cagatgcaat
     4321 actaacggct aataaatcaa gaaaaggggt aatgttgtca aagtcaatat gattatttat
     4381 agcaaaatta ttttagaaag taagccctcg ctgtccacca atgtaa
//
LOCUS       DAOPUR010000056         4414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_19937_length_4414_cov_3.975912, whole genome shotgun sequence.
ACCESSION   DAOPUR010000056 DAOPUR010000000
VERSION     DAOPUR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19937_length_4414_cov_3.975912"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..942)
                     /locus_tag="PKN90_01545"
     CDS             complement(112..942)
                     /locus_tag="PKN90_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ84997.1"
                     /translation="MLFKRYVFCVFFLLSVFPIHALVMQQIENPRSLALGKSYVMLLD
                     NNYALANQASASFLSSSTLALSYHNRFVVKELSSISARYSMPTPWMNVGFLYRFFGFS
                     LYHEMIAGVSVAKALSSHFSLGAQVDYVSVYVSPEEGYLSTFVPQLGIIIKATPSVNL
                     GIHAYNFTFANLQTSYQEEYLQPYLTAGLDYHMDQKVLLLAQIHKPLREESTLSAGIE
                     YTIAPEVIVRTGMSLQQYLQPSFGVGLNIDVLQFDVGFQYHPVLGMSVSSGVVFIFGK
                     "
     gene            1072..1671
                     /locus_tag="PKN90_01550"
     CDS             1072..1671
                     /locus_tag="PKN90_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNZ84998.1"
                     /translation="MKNKLFLLGNIIVLLSLSLPTLATSYIQTAQGYILPGIVVGNDT
                     IPIVVLNEHIVYPKYVKKRDQQKYNKLVRDIKKVYPFAKVVGKEVERVNPLLEKLPQK
                     ERKKYMREYEKALFKQYEPDLKKLTLTQGKLLIKLIDRECEQSSFDLIREYRGGLTAF
                     FWQGFARILGANLKEDFNDAKSEDAMINRIIILIENGQL"
     gene            1746..2156
                     /locus_tag="PKN90_01555"
     CDS             1746..2156
                     /locus_tag="PKN90_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNZ84999.1"
                     /translation="MLLIILFICLGIVIYLLNKYTRRETPAKIADTLTPIIEDTEVCC
                     GAHEVCEKDSLLASSPNAVYFEDEDLDIFRFRDEKSYNAKEITLFEEVFFTLREDEIA
                     EWLKSLQIRNINLPYAIKEEALLIVSERRYAHTH"
     gene            2184..3347
                     /locus_tag="PKN90_01560"
                     /pseudo
     CDS             2184..3347
                     /locus_tag="PKN90_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
     gene            3564..4256
                     /locus_tag="PKN90_01565"
     CDS             3564..4256
                     /locus_tag="PKN90_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85000.1"
                     /translation="MNKRSIILLSVFMAITFFGLLFLQIKYLNSLLDMRVQHFNEAAK
                     RSLYQLSKTLEEEETLHYFNEALAQRPSQRSTSFIDQTVGLQSASSITIQENLQGSTT
                     TIQQQISISSSPSTTRTVQQISETLQNRLKENFAQKKILLDDIAMRWLKESTDKPIEK
                     RIDFTHMENQLKKELENNGITTPFKVILIDYTGEEVYRTSNFNVPNMEHYFSQLLFPN
                     DFNPLRWRKSAK"
BASE COUNT         1671 a          815 c          740 g         1188 t
ORIGIN      
        1 gattctatct attgtttcta aataagtatt ctgagccata ctaacgttgg agggtaaaaa
       61 tccaaaccca caaagcaaga tgcacaaaca ttgacagtat ttcatggagt cctatttacc
      121 aaatataaat acaacacccg aactgaccga catgcccaac actggatgat attgaaatcc
      181 tacatcaaat tgcaaaacat caatgtttaa tcctacccca aaacttggtt gtagatattg
      241 ttgaagagac attccagtac gaacaatcac ttctggagca attgtgtatt caataccagc
      301 cgacaaggta gactcttctc tcaaaggctt atgaatttgg gcaagtaaaa gcactttctg
      361 atccatgtga taatctaaac ctgcagtcaa atagggctgt agatattctt cttgataact
      421 ggtctgcaag ttagcaaaag taaaattata tgcatgtata cctaggttaa cagatggagt
      481 agcttttatg attatcccta attggggaac aaaagtacta aggtaaccct cttctggaga
      541 tacatacaca gaaacataat caacttgtgc tcccaacgaa aaatgagaag acaacgcctt
      601 agcaacagac acaccagcaa tcatctcatg ataaagagag aaaccaaaaa aacgatataa
      661 gaaaccaaca ttcatccacg gagtaggcat agaatagcgg gcactaatcg aacttaattc
      721 tttaacaaca aatcggttat gataagataa agcaagagtt gaggaagaaa gaaatgaagc
      781 agaggcctga ttggctaaag cataattatt atccaacagc atgacataag acttaccgag
      841 agcaagactt cgtggattct ctatctgttg catcactaac gcatgaatag ggaatacact
      901 gagcaaaaaa aacacacaga atacatatct tttaaacaac acaagatctc ctttttttaa
      961 tgaaagacaa aggtaatgtt aagtattctt aatctgcaat agagagtggt aatttttatc
     1021 aaataatact gtatttttgc gtaaatatcg tgtccttata ttctcacgat tatgaaaaat
     1081 aaactattcc tcttgggcaa cataatagtg ttgttgtctc tttctttacc aaccttagca
     1141 acatcgtaca tacaaactgc tcaaggttat attcttcctg ggatagtggt tggtaatgac
     1201 acaatcccta tcgtagtact aaatgaacac atagtttatc ctaaatatgt aaaaaaacga
     1261 gatcaacaaa aatacaataa attagtacga gacataaaga aggtatatcc ttttgccaaa
     1321 gttgtaggga aagaagtgga acgggttaat cctttattag aaaaacttcc gcaaaaagaa
     1381 cgaaaaaaat atatgcgaga atacgaaaaa gccctattca aacagtatga acccgattta
     1441 aaaaaactaa ccctaacaca aggcaaatta ttgattaaat tgatagatag agaatgcgaa
     1501 caaagttctt ttgaccttat tcgcgaatac agaggaggat tgaccgcatt cttttggcag
     1561 ggttttgctc gtatattagg agcaaacctg aaagaagact ttaatgatgc aaaaagtgaa
     1621 gacgcaatga ttaatcggat aattatattg atagaaaacg gacaattata acacaaagaa
     1681 atacgattca taaaaataaa atcattatct ttgcaacatt agatgaacat accaaacctt
     1741 aacctatgct actcattatc ctttttatct gtttagggat agttatctat ctcctcaaca
     1801 aatacacacg aagagaaact ccggcgaaaa tagccgatac gctaacgcca ataatagaag
     1861 atactgaagt atgttgtgga gcacatgaag tatgtgaaaa agactcctta ttagcttcgt
     1921 cccctaatgc tgtttatttc gaagatgaag acctagatat tttccgattt cgcgacgaga
     1981 aatcatataa tgcaaaagaa attacactat ttgaggaagt attttttaca ttgcgtgaag
     2041 atgaaattgc cgaatggcta aaaagcttgc aaatcagaaa cattaatctc ccttatgcta
     2101 tcaaagaaga agctctcctt attgtatctg aaagaagata cgcacatact cactaagaat
     2161 attatactag ctcaccaaac atcatgaaca aagaatttcc gttaattgca aaaacattac
     2221 agggtctcga agatatttta gcaaaagagc tagcacaaat aggagcaaac aatatacaaa
     2281 aagaaaaaag aggtgtctcc tttacaggaa ataacgagtt gatgtacaag gcaaatcttc
     2341 actgtcgtac agcaattcgt attctaaaac ctattttcac atttcaagca aaaaatgcag
     2401 atgaaatata cgcccaaata aaaaaaatac attgggaaaa atacttttca gcgaaagaaa
     2461 gttttgctat cagtgctgtt gtatactcag aaacatttag ccactcaaaa tttgttacgt
     2521 ataaagttaa agatgccatt gtagattatt ttaaagaaaa atttaacgag cgtccttcaa
     2581 ttaagattac aaacccagac gtatatttaa atattcacat tgcacacact acttgcacaa
     2641 tatcactaga tagttcggga gaatcattac acaaacgagg ctatcgaagt gctcagacag
     2701 aagcacctct caacgaagtg ttagcagcag gtttaattat gctcacagga tggaaaggcg
     2761 aatcggattt tctcgacccc atgtgtggat ctggaacact cctcattgaa gctgctatga
     2821 tagcacttaa tattccgccg ggagtcttta gaaaagaatt tgcgtttgaa aaatggaaag
     2881 atcttgacag tgaagtattt gatagagtgt ataatgacga tagcaacgaa cgcattttta
     2941 agcataaaat ttacgggtcg gacatttcag ctcaagctct aaaaataaca gaagaaaatt
     3001 gcaaaagtgc aggattaagt aaatatatta gcttgcaaaa agccaatttt atggaactcc
     3061 ccactccaac acaaaaaaca cttatcgtta ccaatcctcc ttacggagaa cgcattgggg
     3121 gcaatagcat caataagcta tatgcagaaa taggcgaaac gctaaaacat cgtttccctg
     3181 gagccgaagc ctggattatt agctcaaaca tggaaggatt ttataaaatt ggactccgcc
     3241 catcacaaaa atatctactc ctaaatggtt caatcgaatg tgaataccgc aaatacgaac
     3301 tgttcgatgg gaaaatgaaa gacaaaaaag cagcaataaa tcaggaataa tcaaacgcca
     3361 ctacgtacgg tatcatttaa caggctatcc cacatagtaa atgttaaatg atactaaaga
     3421 aagtttgaat tcaaatgtta aaatcatatc tttgttttgt taaaaagaag atatgaaatg
     3481 ctctaataaa catatttcaa cccagaggtt ggcaataccg caagttccca atacccttaa
     3541 cgtaaaaact taagaataag cctatgaata agcgaagtat aatccttttg tcagtcttta
     3601 tggcaattac cttttttggg ttattgtttt tacaaataaa gtacctcaac tctttgttag
     3661 atatgcgtgt gcaacatttt aacgaagcag caaaacgcag tttataccaa cttagcaaaa
     3721 cactagaaga agaagaaaca ctacactatt tcaatgaagc cctcgctcaa cgtccttctc
     3781 aacgaagcac cagttttatc gaccagacag taggattgca aagtgcaagt tctattacta
     3841 ttcaagaaaa tctacaagga agtaccacta ccattcagca acaaatatca atttcaagct
     3901 cgccctctac aacacgaaca gtacaacaaa tatcagaaac acttcaaaat cgattaaaag
     3961 aaaattttgc tcaaaaaaag attttacttg atgatattgc tatgcgatgg ttgaaggaat
     4021 ctactgacaa accaatcgaa aaacgtattg attttacaca tatggaaaat caattaaaaa
     4081 aggaactaga aaacaatgga atcactaccc cattcaaagt tattttgatt gactatacag
     4141 gagaagaggt ataccgaaca tccaatttta atgtgccaaa tatggaacat tatttctcac
     4201 aactcctatt tccaaacgat ttcaatccgc ttcggtggag gaaatccgca aaataaataa
     4261 tatggaatcc aatgaacgtt tgacggtggg taagaccttg cgtattccta aataatgatg
     4321 atagcaacac ttcagcgcat tgatcttgat acaggaggca tcttacttgt ccataagcct
     4381 ttacgttgga cttctttcga tgttataaac aaaa
//
LOCUS       DAOPUR010000057         4412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_19956_length_4412_cov_3.108561, whole genome shotgun sequence.
ACCESSION   DAOPUR010000057 DAOPUR010000000
VERSION     DAOPUR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19956_length_4412_cov_3.108561"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /gene="sufB"
                     /locus_tag="PKN90_01570"
     CDS             <1..872
                     /gene="sufB"
                     /locus_tag="PKN90_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNZ85001.1"
                     /translation="FVYIPKGVRCPMELSTYFRINAANTGQFERTFIVAEEDSYVSYL
                     EGCTAPMRDENQLHAAIVEIVILDRAEVKYSTVQNWYPGDKNGVGGIYNFVTKRAICK
                     GDSAKVCWTQVETGSAITWKYPSCILKGDNSIGEFYSVAVTNNYQQADTGTKMIHLGK
                     NTRSRIVSKGISAGKSQNSYRGMVKVQKGAENARNFSQCDSLLLSDRCGAHTFPYIEV
                     NNDTAIVEHEATTSKISEDQIFYCNQRGINTEDAVGLIVNGFAKEVLNKLPMEFAVEA
                     QKLLQISLEGSVG"
     gene            920..1672
                     /gene="sufC"
                     /locus_tag="PKN90_01575"
     CDS             920..1672
                     /gene="sufC"
                     /locus_tag="PKN90_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNZ85002.1"
                     /translation="MLVIKDLHANVNGKEILSGINLTVNPGEIHAIMGPNGSGKSTLS
                     AVLAGNPVFEVTKGEVYFQGKNLLEMAPEDRAREGVFLSFQYPVEIPGVSMVNFMRTA
                     INEHRKYNNLEPISASDFLKLMREKKEIVELDTKLTNRSVNEGFSGGEKKRNEIFQMA
                     MLNPKLSILDETDSGLDIDALRIVANGVNQLKNSDNASIVITHYQRLLEYIVPDFVHI
                     LYKGRIVKTAGKELALILEEKGYDWIKQEIEE"
     gene            1674..3014
                     /gene="sufD"
                     /locus_tag="PKN90_01580"
     CDS             1674..3014
                     /gene="sufD"
                     /locus_tag="PKN90_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNZ85003.1"
                     /translation="MVKQQYITLYQQYAHIIKEKSAPLLNSIRDAAFKRFEELGFPVK
                     RDKDYEYIDIQSSYDHDFGLNLNRVSLDVDPYKVFTCDVPNIQSYLCFIVNDTFYANP
                     EPKISLPKGVIVASLNEICTTHPDLVKPYLFKNMDWENDPNQAFNATFAQDGLFIYVP
                     KNVVVDVPIQLVNLMHGNDDLLAVNHNLIIVEEGASVKLLVCDHASNEKKYLANRLTE
                     IFVGKNASYEHYKIENTHNQTMNLGTVLVHQDDDSRFLSNTLTLHNGITRNNITVHVD
                     GQNAEAIVGGLAIGDKTQKIDNYLSMNHLKPNSTSLQLFKYILDDQAKGVFTGKIIIK
                     EDAQKIAAYQTCKTICLTNTATINTKPQLEIFADNVKCSHGATVGQLDETALFYLRSR
                     GIPINEAKLLLMYAFVHDVLEHISVDAVKDRIRLLVEKRLRGELSKCNGCVICK"
     gene            3033..4256
                     /locus_tag="PKN90_01585"
     CDS             3033..4256
                     /locus_tag="PKN90_01585"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNZ85004.1"
                     /translation="MSINNQLIRQDFPILLDKIYNKPLVYFDNAATTQKPMCVIDRIV
                     HGYTHGNANIHRGVHFLSQKATEEHENARTTVQHFIHAARSSEIIFTRGTTESINLVA
                     FSFGETFCQPGDEIIISAMEHHANIVPWQMLKDRKGIVLKVIPMLPDGTLDMPAFSKL
                     LNNKTKLVAVTHVSNVLGTINPIQEIIQLAHNSNVPVLIDGAQAVPHIAIDVQQLDVD
                     FYVFSAHKVYGPTGIGVLYGKEEWLNKMQPYQGGGEMIAKVTFEKTTYNELPFKFEAG
                     TPDYIGSTALATALEYIQQIGLNAIATYENDLLQYATAKLLQVPGLKIYGEASHKSSV
                     ISFLVQGIHHYDMGTMLDKLGIAVRTGHHCAQPIMDYYGIEGTVRASFAFYNTKEEVD
                     KLIEGVNRVVSMFKI"
     gene            4268..>4412
                     /locus_tag="PKN90_01590"
     CDS             4268..>4412
                     /locus_tag="PKN90_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNZ85005.1"
                     /translation="MTIQEKQDIIIEEFSVFDDWFDKYALIIEYANKLKPLDEKKKTP
                     QNII"
BASE COUNT         1557 a          696 c          854 g         1305 t
ORIGIN      
        1 cttttgtgta tataccaaaa ggagtgcgtt gtcccatgga gttatcaacc tattttagaa
       61 taaacgcagc taatacaggt caatttgaac gcacctttat tgttgcagaa gaagattcgt
      121 acgtttctta tctagaggga tgtacagctc caatgagaga tgaaaatcaa ctgcatgcag
      181 ccatcgtaga gattgtgata ttagataggg ctgaagtaaa atattcgacc gtacaaaatt
      241 ggtatccagg tgataaaaat ggcgtaggtg gtatatataa ttttgttacg aaacgtgcta
      301 tttgcaaagg agatagtgct aaagtttgtt ggacccaagt ggaaacaggt tctgcaatta
      361 cgtggaaata cccaagttgt attcttaaag gagataattc tatcggagaa ttctattctg
      421 tagctgtaac taacaactac caacaagcgg ataccggtac caaaatgata catctaggca
      481 aaaataccag aagtcgtatt gtgtctaaag gaatatctgc aggaaaaagt caaaactcgt
      541 atagaggaat ggtaaaagtg caaaaagggg cagaaaatgc acgtaatttt tcgcaatgcg
      601 atagcttatt gcttagcgat agatgtggag cacatacatt tccctatatt gaggtaaata
      661 atgatacagc tattgttgaa cacgaagcta caacatcaaa aattagcgaa gaccaaatat
      721 tttattgcaa tcaacgtggt atcaataccg aagatgcggt tggtctgatt gtcaacggat
      781 ttgccaaaga ggtgttaaat aaattaccta tggagtttgc cgttgaagct caaaaattat
      841 tgcaaatatc tttagaagga agtgtaggat aaattttaat ataatcttat actttataaa
      901 ttttataaat aatatcaata tgctagtaat aaaagattta catgccaacg taaatggcaa
      961 agaaatattg tcgggaatta atttaactgt taatccagga gaaattcatg ctattatggg
     1021 tccgaacggt tcaggaaaaa gtactttatc tgccgtttta gcaggaaatc cagtatttga
     1081 agtaacaaaa ggagaggttt atttccaagg aaaaaacctg ctcgaaatgg ctcccgaaga
     1141 ccgtgcaaga gaaggggttt ttcttagttt tcaataccca gtagaaatac ctggtgttag
     1201 tatggttaac tttatgcgta ctgctatcaa cgaacatcgt aaatacaata accttgaacc
     1261 aatttctgca agcgatttct taaaattgat gcgagaaaaa aaggaaatag tagaacttga
     1321 taccaaatta acgaatcgtt cggtaaatga aggtttctct ggtggcgaaa agaaaaggaa
     1381 tgaaattttt caaatggcta tgctaaatcc aaaattgtct attttagatg aaacggattc
     1441 tggattagat atagatgcct tacgtattgt agctaatgga gttaatcaat tgaaaaatag
     1501 cgataatgct tccattgtga ttactcatta tcaacgatta cttgaatata tcgtccccga
     1561 ttttgttcat attttataca aaggtcgaat tgtaaaaacg gccggcaagg agttagctct
     1621 tatcctcgaa gaaaaaggat acgattggat taaacaagaa attgaggaat agtatggtaa
     1681 agcaacaata tataacgtta taccaacaat acgcacatat cattaaagaa aaaagtgctc
     1741 cattgttgaa tagtattcgt gatgctgctt ttaagcgttt cgaagaactg ggttttcccg
     1801 tcaaacgaga taaagattac gaatacattg atattcagtc ttcatacgat catgattttg
     1861 gattaaatct aaatagagtc agtttagatg ttgatccata taaagtgttt acttgcgatg
     1921 ttcccaatat acaatcgtat ttgtgtttta ttgtcaatga tactttttat gccaatcctg
     1981 aaccaaaaat ttccttacct aagggcgtaa ttgtagctag tttaaatgaa atttgcacca
     2041 ctcaccccga tttagtaaaa ccctatctat ttaaaaatat ggattgggaa aatgatccaa
     2101 accaagcttt taacgcaact tttgctcaag atggattgtt tatttatgtg cctaaaaatg
     2161 tggttgttga cgtgccaatt cagctagtaa acttgatgca tggaaatgat gatttattgg
     2221 cagtgaatca caatcttatt attgtggaag aaggtgcaag tgttaaattg ttagtatgcg
     2281 atcatgcttc aaatgagaaa aaataccttg ccaatagatt aacagagatt tttgttggca
     2341 aaaatgcttc ttacgagcac tataaaattg aaaataccca caatcaaaca atgaatcttg
     2401 gtaccgtgtt agtgcatcaa gacgatgata gtagattttt atcaaatacc cttactttgc
     2461 ataacggaat aacccgtaat aatatcacgg tacatgtaga cggacaaaat gccgaagcaa
     2521 tagtaggagg actagccatt ggagataaaa cgcagaagat agataactat ttgtcgatga
     2581 atcacttaaa accaaactct actagtttgc aattgtttaa gtatatactc gatgatcagg
     2641 caaaaggtgt gtttacaggc aaaattatta taaaagaaga tgctcaaaaa attgctgcat
     2701 atcaaacgtg caaaactata tgtttaacaa atacggcaac aattaacaca aaaccacaat
     2761 tggaaatttt tgctgacaat gtgaaatgta gtcatggtgc aaccgtaggt cagctcgatg
     2821 aaacagctct tttctatctc cgttcaagag gtattcctat taatgaggct aaactgttgc
     2881 ttatgtacgc atttgtacac gatgtgttgg aacacatttc agttgatgca gtcaaagacc
     2941 gtattagatt gttagtagaa aaaagattac ggggagaatt gtctaaatgt aatggatgtg
     3001 taatttgtaa ataaggatga taaataaaaa aaatgagcat aaataatcaa cttatccgac
     3061 aggatttccc aattctgtta gataaaattt acaataaacc attagtttac ttcgataatg
     3121 ctgctaccac acaaaaacca atgtgtgtga ttgatagaat tgtacatgga tatacgcatg
     3181 ggaatgcaaa tatccacaga ggtgttcatt ttttaagcca aaaagcaacc gaagaacatg
     3241 aaaatgcacg tacaactgtg caacatttta ttcatgccgc aaggtcgagt gaaataattt
     3301 ttactcgagg tactactgag tctattaatt tagtggcatt ttcttttggc gaaacgtttt
     3361 gccaaccggg ggatgaaatt ataatttctg ctatggaaca ccatgcaaat attgttcctt
     3421 ggcaaatgct taaagataga aaagggattg tgcttaaagt tattccaatg ctaccagatg
     3481 gaactttgga tatgcctgct ttttcaaaac tattgaataa caaaacaaaa ttagttgcag
     3541 ttactcatgt ttcaaacgtg ctaggaacaa taaacccaat tcaagaaatt attcaactcg
     3601 ctcataattc caatgttcca gtgttgattg atggagcaca agctgtacca catattgcta
     3661 ttgatgtcca acaattggat gttgattttt acgtgttttc tgcacacaag gtgtacggtc
     3721 ctactggcat tggcgtattg tatggaaaag aagaatggtt aaataaaatg caaccatacc
     3781 aaggaggagg agaaatgatt gcaaaggtta ccttcgaaaa aactacttat aacgaacttc
     3841 cgttcaagtt cgaagcaggt acgcccgatt atattggttc tacagccctt gctacagcct
     3901 tagaatatat ccaacaaatc ggattaaacg ctattgccac atacgaaaac gacttactac
     3961 aatatgcaac agcgaaactg ttgcaagtac cagggttaaa aatttatggc gaagcatcgc
     4021 ataaaagcag tgtgatttct tttttagtac aaggcattca tcattacgat atgggaacta
     4081 tgctcgacaa gctcggtatt gcggtccgaa caggtcatca ttgtgcacag ccaattatgg
     4141 attattatgg catagaggga acggtaaggg cttcatttgc tttctataat accaaagaag
     4201 aggtagataa actcatagaa ggagtaaata gggtggtatc tatgtttaaa atttaatttg
     4261 taagggtatg acaattcaag aaaaacaaga tataataata gaggaatttt cagtttttga
     4321 cgattggttt gataaatatg cactcattat tgagtatgcc aataaattga aacctcttga
     4381 tgagaaaaaa aaaacaccac aaaacatcat cg
//
LOCUS       DAOPUR010000058         4408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_19977_length_4408_cov_3.749139, whole genome shotgun sequence.
ACCESSION   DAOPUR010000058 DAOPUR010000000
VERSION     DAOPUR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19977_length_4408_cov_3.749139"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKN90_01595"
     CDS             complement(<1..377)
                     /locus_tag="PKN90_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNZ85006.1"
                     /translation="MLKKYISFDFRKVFSQPLMVNVASLGILQLANYIIPIIIIPFVV
                     RALGVEIFGKVSYAQNIITYLTIVVNYGFEYSATQDVAINRDDRNKLSTVFWTTIRFK
                     FCLLLITFLLLLILFFTFDKVKE"
     gene            complement(396..1877)
                     /gene="proS"
                     /locus_tag="PKN90_01600"
     CDS             complement(396..1877)
                     /gene="proS"
                     /locus_tag="PKN90_01600"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNZ85007.1"
                     /translation="MAKELKDLTKRSENYSQWYNDLVVKADLAEQSAVRGCMVIKPYG
                     YAIWEKMQQELDRMFKETGHVNAYFPLLIPKSFLSKEAEHVEGFAKECAVVTHYRLKN
                     AEDGSGVVVDPAAKLEEELIIRPTSETIIWNTYRNWIQSYRDLPILCNQWANVFRWEM
                     RTRMFLRTAEFLWQEGHTAHATKVEAEEEALKMLHVYGDFAENFMAVPVVKGIKTANE
                     RFAGALETYTIEAMMQDGKALQSGTSHFLAQNFAKAFDVQFITKENKQEYVWATSWGV
                     STRLMGALIMTHSDDNGLVLPPKLAPIQVVIVPIYKGEEQLQAINEKVDGIVKKLKAL
                     GISVKYDNADNKRPGFKFAEYELKGVPVRLVMGGRDLESNTIEVTRRDTFEKQTRSCD
                     DIDTFVKQLLDDMQQTILQKALDLRTSLTCTVETYDEFKEKIEEGGFILAHWDGTSET
                     EEKIKEETKATIRCIPLEGDKTPGVCMVTGKPSAQRVLFARSY"
     gene            2024..3916
                     /locus_tag="PKN90_01605"
     CDS             2024..3916
                     /locus_tag="PKN90_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNZ85008.1"
                     /translation="MAVINYIQLENVSKRFGDLLLFEGLNMDISEGQRVALIAKNGTG
                     KSTLLNIIMGKESLDEGKITFRRDITLSFLEQDPSYNPEQTILDTFFSSNNETIRLIA
                     QYEAYISKNENLDSPEFQELVEQMEIRKAWDYDIKIKQILAQLKITDFDKKIKHLSGG
                     QLKRVALANALITEPDLLILDEPTNHLDLEMTEWLEEYLIRSGLTLLMVTHDRYFLDR
                     VCTDIIEIDQKQLYAYKGNYSYYLEKRQDRIDAQNAELARANNLFRKELDWMRRQPQA
                     RATKAKSRIDSFYDLEKKVAQKRQDESVKLQMNATYLGSKIFEAKYISKQFDDLVLIK
                     DFYYNFSRYEKMGIIGKNGTGKSTFLKLLLGIIKPDSGSFDIGETVSFGYYSQDGLTF
                     DEQMKVIDVVRDIAEEINIGNGKKLSASQFLQHFLFTPETQHNYVYKLSGGEKRRLYL
                     CTVLMKNPNFLVLDEPTNDLDIVTLNVLEEYLANFKGCVIVVSHDRYFTDKVVDHLLV
                     FQGNAVIKDFPGNYSDYREWKEQQDAIDAKQAEIKQKNKVEEPKRSVNRTRKLSFAEQ
                     REYSLLESDIENLETEKKDLETMLSGGNIPQQELIKASERIAEVIKLIDDKTMRWLEL
                     SEYTNN"
     gene            3949..>4408
                     /locus_tag="PKN90_01610"
     CDS             3949..>4408
                     /locus_tag="PKN90_01610"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85009.1"
                     /translation="MKTWNYLLLTAVLLLTACSAKEKEPEIIKYTVTIEQPEGGTITV
                     KQGNTVIENGTEIIKGTSLTVTATPDEFYTLESVNVSSPFELTQDTTITAVFEPMVMY
                     TVTIEQNEQGTITVLNKGNVVNSGDQVPENTELSVIAFGNNSHVFSDINTT"
BASE COUNT         1504 a          819 c          755 g         1330 t
ORIGIN      
        1 tcttctttca ccttgtcaaa agtgaaaaac aaaataagaa gaagcaaaaa agtaattaaa
       61 agtaaacaaa acttaaatcg aatagtagtc caaaatacag tgcttaattt atttctatca
      121 tctctattga tggcaacatc ttgtgtagcc gaatactcaa atccataatt taccacaata
      181 gttagatatg ttataatatt ttgagcataa gaaacttttc caaaaatttc aacacccaaa
      241 gctcttacaa caaaaggaat gataataata ggaattatat aatttgccag ttgtaaaatc
      301 cccaaagagg ccacatttac cattaagggt tgggaaaata cttttcggaa atcaaaagag
      361 atgtattttt taagcattac gccacaatat tttgattaat acgaacgtgc aaacaatacc
      421 ctttgtgcag aaggttttcc tgtaaccata cacacaccag gagttttatc tccctctaac
      481 ggaatacaac gaattgttgc tttggtttct tctttaattt tttcttcagt ttcggaagta
      541 ccatcccaat gagcaagtat aaaaccacct tcttcaattt tttctttaaa ctcgtcgtac
      601 gtttccacag tgcaagttag agatgttctc aaatcaagtg ctttttgcaa aatagtttgt
      661 tgcatatcat ccaacaattg tttcacaaaa gtatctatat catcgcaact acgtgtttgt
      721 ttttcaaacg tatctctacg agtaacttct atggtattgc tttctaaatc gcgaccaccc
      781 attaccaatc gtacaggcac tcctttgagt tcgtattcgg caaacttaaa tcctggtcgt
      841 ttgttgtcag cattgtcgta ttttacactt atgcccagtg ctttcaattt ttttacgata
      901 ccatctactt tttcgttaat ggcttgtaat tgttcttctc ctttataaat aggtacaatt
      961 accacctgaa taggggctaa ttttggaggc aataccaatc cattatcatc gctatgtgtc
     1021 attatcaatg ctcccatcaa acgggtagaa acaccccacg aagtagccca cacatattct
     1081 tgtttgtttt ctttggtaat aaactgcaca tcaaaagctt tcgcaaaatt ttgtgctaaa
     1141 aagtgcgaag tgcccgattg taaggctttt ccatcttgca tcatagcttc gatggtatag
     1201 gtttcgaggg ctccagcaaa acgttcgttg gctgttttta tgccttttac aacaggaact
     1261 gccataaaat tctctgcaaa atcgccatat acgtgcagca tttttaatgc ttcttcttca
     1321 gcctctactt tagtagcatg agcagtatgc ccttcttgcc acaaaaattc tgctgtacgc
     1381 aaaaacatac gtgtacgcat ttcccaacga aatacgtttg cccattgatt acataaaatg
     1441 ggtaaatcgc ggtacgattg tatccaattt cggtaagtat tccaaataat agtttcggag
     1501 gttggacgaa taattaattc ctcttctaac ttggcagctg ggtcaaccac tacacccgat
     1561 ccatcttcag cattttttaa tctataatga gttactacag cacattcttt agcaaaacct
     1621 tctacatgct cagcttcttt acttaaaaaa gatttgggaa ttaataatgg aaaatatgca
     1681 ttcacatgac ctgtttcttt gaacatacgg tctaattctt gttgcatttt ttcccaaata
     1741 gcataaccat agggcttaat taccatacaa ccccttactg ctgactgttc agctaaatca
     1801 gcttttacta ctaaatcgtt gtaccactgc gaataatttt cgctacgttt ggtcaaatct
     1861 tttaattctt ttgccatttt tacactcaaa ttttgagcca caaaagtaat aaaaattaac
     1921 caataaggct attttttaga caagatttgc atgtatcgtt tttggagtat tgctacttta
     1981 gagttttata tgtacatttg ctcgttattt ataaaaagac ttaatggcag taattaacta
     2041 catacaactt gaaaatgtga gtaaacgctt tggcgattta ctgcttttcg aagggctaaa
     2101 tatggatatt agcgaggggc aacgcgtagc cctaatagca aaaaacggaa ctggcaaatc
     2161 caccctactc aatataatca tggggaaaga atccttagac gaagggaaaa taacctttcg
     2221 ccgggatata acgctttctt ttttagagca agacccatct tacaatccag aacagactat
     2281 attggatacg tttttttcgt cgaacaacga aacaattcgc ctaattgcac aatacgaagc
     2341 atatatcagc aaaaacgaaa atttagatag cccagaattt caagaattgg tagaacaaat
     2401 ggaaattcgt aaagcttggg attatgatat taagattaag caaattttag cacaactcaa
     2461 gattactgat tttgacaaaa agattaaaca cttatctggc ggtcaactca aacgggtagc
     2521 tttggctaat gctttgataa cagagcccga cttgcttatt ttagatgagc ctaccaacca
     2581 tttggattta gaaatgaccg aatggttaga agagtacctt attcgttcgg gattaacctt
     2641 attgatggta actcacgata ggtatttttt agaccgtgtg tgtactgaca ttatagaaat
     2701 agaccaaaaa cagttatacg catacaaagg caattacagc tattatttag aaaaaagaca
     2761 agaccgcatt gatgcccaaa atgctgagtt agcacgtgca aacaatttat tcagaaaaga
     2821 attagattgg atgcgacgac aacctcaagc aagggcaacg aaggcaaaaa gcagaattga
     2881 cagtttttat gatttagaaa aaaaagtggc acaaaaacgt caagatgaat cggtaaaact
     2941 gcaaatgaac gccacttatt tgggttcaaa aatttttgaa gccaagtata ttagcaaaca
     3001 atttgacgat cttgtgttga taaaagattt ttactataat ttttcccgat acgagaaaat
     3061 gggcataatt ggtaaaaatg gtacaggaaa atctactttt ttaaaattgc ttttaggaat
     3121 tattaaaccc gacagtggct catttgatat tggcgaaacc gtttcttttg gctactacag
     3181 tcaagatggg ttaacgtttg atgaacaaat gaaagtgatt gatgtagtac gcgacattgc
     3241 cgaagaaata aatataggaa acggaaaaaa actaagtgca tcccaatttt tgcaacattt
     3301 tttgtttaca cccgaaacac aacataatta tgtgtacaaa cttagtggag gagaaaaacg
     3361 ccgcctttac ctatgtactg ttttgatgaa aaaccccaat tttttggtgc tcgacgagcc
     3421 taccaacgac ttggatattg ttaccctaaa tgtattggaa gaatatttag ctaattttaa
     3481 gggctgcgtg atagtcgttt cgcacgatag gtattttacc gacaaggtag ttgatcactt
     3541 attggtattt caaggcaatg ctgttatcaa agattttcct ggtaattact ccgattaccg
     3601 agaatggaaa gagcaacaag atgctattga tgccaaacaa gcagaaatta aacaaaaaaa
     3661 taaagttgaa gaacccaaac gctctgttaa ccgtacaaga aagttgagtt ttgccgaaca
     3721 acgtgaatat tcattgctgg aatcagacat agaaaatttg gaaactgaaa aaaaagacct
     3781 tgaaacaatg ttatcaggag gaaatattcc tcaacaagag cttataaaag catccgaacg
     3841 tattgctgaa gtgataaaac taattgacga taaaacaatg cgatggttag agctaagcga
     3901 atacaccaac aattaaataa ctttttaatc aattaataac ctatacgtat gaaaacatgg
     3961 aattacttac tgcttacagc agttcttctt ttaacagcat gtagtgctaa ggaaaaagaa
     4021 ccagaaataa tcaagtacac cgttactatt gaacaacccg aaggcggaac cattaccgta
     4081 aaacaaggaa ataccgtaat tgaaaacgga actgaaataa taaaaggcac cagccttacc
     4141 gttacagcta cccctgatga attttacacc ttggaaagtg taaacgtaag ttcacctttt
     4201 gagctaaccc aagataccac aattacagca gtatttgagc caatggttat gtatacggtt
     4261 acgattgaac aaaacgagca aggaactatt acggtactga ataaagggaa tgtagtaaat
     4321 agtggagacc aagtgcctga aaacacggaa ctttctgtga ttgcttttgg caataatagc
     4381 catgttttta gcgatatcaa tacaacac
//
LOCUS       DAOPUR010000059         4372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_20192_length_4372_cov_3.017836, whole genome shotgun sequence.
ACCESSION   DAOPUR010000059 DAOPUR010000000
VERSION     DAOPUR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20192_length_4372_cov_3.017836"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKN90_01615"
     CDS             <1..714
                     /locus_tag="PKN90_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNZ85010.1"
                     /translation="AAAIAHFTCHAWGVAHTVYSIPFVPELTPVSLVSAIAAGIIFGI
                     VAMLFSKTGHWWTGLFSRFIPYPPLRPFAGGMIIAMAVWMLGTTKYIGLGVPTIQASF
                     DTEMQGYDFLLKLLFTTFTIGAGFKGGEVTPLFFIGATLGNVLVWFLPLPMALLAGMG
                     FVAVFAGATNTPLACTMMGIELFGAESGLYIGIACVVAYFFSGHTGIYAAQKVGSPKH
                     VAFLKQQGESEAETRSVRS"
     gene            complement(717..1250)
                     /locus_tag="PKN90_01620"
     CDS             complement(717..1250)
                     /locus_tag="PKN90_01620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNZ85011.1"
                     /translation="MNILAFGASHSKSSINQQFASYVAQLFSAGNQVSVIKLTDFDPP
                     VYTVDREAESGFPEAVKQFLLQLQKADLIIISLAEHNGSYTASFKNLFDWTSRVEMNL
                     FANKKLFLLSTSPGPNGGKFVMEAAKVRFPIHGAAIVGSFSLPRFADNFDAQKGITDT
                     ELKEAFDTEIEKVTTAL"
     gene            complement(1342..1629)
                     /locus_tag="PKN90_01625"
     CDS             complement(1342..1629)
                     /locus_tag="PKN90_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="insecticidal delta-endotoxin Cry8Ea1 family
                     protein"
                     /protein_id="HNZ85012.1"
                     /translation="MRYVPIDLAASIAAGEEHLASTDIYTQRFNDYFRCDVRISFKQN
                     LGKVSQEWGLDLQNVSNYKNIFAQQYNKYSESIATVYQQGFMPMMMYRIHF"
     gene            complement(1639..3720)
                     /locus_tag="PKN90_01630"
     CDS             complement(1639..3720)
                     /locus_tag="PKN90_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ85013.1"
                     /translation="MKKLFLLISTFFLIQAALFSETGQTVRGVITDAFSGQPLIGASI
                     VVADSNPLQGTIADENGKFELNNVPLGRSTFIVSALGYHAASMSNIMVISGKETLLQI
                     ALEEKITSLKDVVVTANFSKNKPINDLALVSSRSFSVEETERFAGSLGDPARMVANYA
                     GVVVGNDSRNDIIIRGNSPSGLLWRMEGVAISNPNHFGAHGTTGGPVTMLNSNLLANS
                     DFMTGAFSAEYGNALSGVFDLNLRTGNTERFEGMGQVGFNGFELLLEGPIKIGNFIKN
                     GSFVADYRFSTLQLMSLLGFDIGTGGTVPEYNDLSVMIDLPTSKIGRFKLIGLLGNSF
                     ISMGRSFDIDEAITHNQMGYATDFGAGLNIAMLTHTYMFNEHSRLKSSLSFEHATSTT
                     LSDSINYTDKKYFNLYAANYKENKWMANTQFKRRINAKNNLTLGASASLFVSSFFDSV
                     FVNSLNKRITLTAIDKEPSILYNAYTNWQHKFTNELSLNTGLHYQLYNLTNESAIEPR
                     LALRWQAHQQHAFSLGYGLHSQIQARTIYFYEAYYKDTDTYARNNMTTGYTRSHHIVG
                     GYDWTISSYWRVKTEAYYQDLFNVPVSPISGSYSALNSGSGYYIANIDSLQNTGVGRN
                     YGLEMTIEKFLHKGYYGLMTFSLYDSKYQGYDKIWRNTAFNSNFAVNLLGGYEWRVGT
                     KNFLAVDVRTI"
     gene            complement(3839..>4372)
                     /locus_tag="PKN90_01635"
     CDS             complement(3839..>4372)
                     /locus_tag="PKN90_01635"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HNZ85014.1"
                     /translation="AGTRISEAAQRVIQDIMDDLTAATINPVKSNEIEKIREVCQSGK
                     MRTVKPTKVDVWLKSHDDELFLFDIKTAKPNKGGFKEFKRTLLEWTACVLANDPKAIV
                     NSLIAIPYNPYEPKPYSRWTMAGMLDLDNELKVAAEFWDFLGGQGAYTDLLDIFERIG
                     LELRPEIDNYFEKYNKK"
BASE COUNT         1276 a          882 c          846 g         1368 t
ORIGIN      
        1 gcggctgcta ttgctcattt tacctgtcat gcttggggtg tagcccatac ggtttactcc
       61 ataccttttg tacccgaact tactcctgtc agcttggtat cggcaatagc ggcgggaata
      121 atctttggga tagtggctat gctattttcc aaaactggtc attggtggac gggtttgttc
      181 tcgcgtttta ttccttatcc acctttgcgt ccgtttgctg gcggtatgat tatcgctatg
      241 gcagtttgga tgttggggac taccaaatac attggattgg gggttcctac catacaagct
      301 tcattcgaca ccgagatgca aggatatgat tttctactaa aattgttatt taccacattt
      361 accattggag ctggtttcaa aggaggagaa gtaactcctt tgttctttat tggtgctaca
      421 ttgggtaatg tgttggtgtg gtttttgcct ttacccatgg cattgcttgc aggaatggga
      481 tttgtggcgg tgtttgcagg agcgactaat acgccgcttg cctgcaccat gatgggaata
      541 gagttatttg gagcagaaag cggtttgtat atcggtattg cttgcgtggt ggcttatttc
      601 ttttcgggtc atacaggtat ttatgctgcc caaaaagtgg gaagcccaaa gcatgtagca
      661 tttctcaagc aacaaggaga aagtgaggct gaaacccgtt cggtgcgatc gtaggtttac
      721 aaagctgttg ttactttttc aatttctgtg tcaaacgctt ctttaagttc cgtgtccgta
      781 atgccttttt gtgcatcaaa attatccgca aaacgaggca atgaaaagct accaacaatt
      841 gctgccccat gaatgggaaa gcgtactttg gcagcttcca ttacaaattt tccgccattt
      901 ggaccaggag aagtagaaag taaaaataat tttttgttgg caaaaagatt catttctaca
      961 cgagaagtcc aatcgaacaa gtttttgaac gatgcggtgt aactaccatt gtgttcggct
     1021 aaggaaataa taatcaaatc tgccttttgt aactgaagta aaaactgttt tactgcttcg
     1081 ggaaaaccac tttctgcctc cctatctacc gtatatacag gaggatcaaa gtcagtaagc
     1141 tttataacag acacttggtt gcctgccgaa aataactgtg caacataact tgcaaattgt
     1201 tggttgatag agcttttgct atggctagct ccaaatgcta gtatattcat gatgtgctat
     1261 ttttttaaag tttataaaac aaaaatagca atttaaaaat gcgtttccaa ttcttttatt
     1321 aacatttctg cttccttaac attaaaaatg aattcgatac atcatcatag gcataaagcc
     1381 ttgttggtaa acggtagcta tactttctga atatttgttg tattgttgtg caaaaatgtt
     1441 tttataattg cttacatttt gcaaatccaa tccccattct tgcgatactt tgcctaagtt
     1501 ttgtttaaaa ctaatccgca catcgcacct aaaataatcg ttgaagcgtt gcgtataaat
     1561 atcagtagaa gctaaatgtt cttcgcccgc tgcaatagag gcagccaaat cgataggcac
     1621 ataccgcata ccgcctgcct aaatggtgcg aacatcaaca gccaaaaagt tttttgtacc
     1681 aacacgccat tcgtaaccac ccaataagtt gacagcaaaa ttggagttaa aagctgtatt
     1741 tctccatatt ttatcatacc cttggtattt tgagtcgtaa agcgagaagg tcattaaacc
     1801 ataatacccc ttgtgaagaa atttttctat ggtcatttcc aatccataat ttcgtccgac
     1861 acccgtattt tgcagactgt caatattggc aatgtagtag ccactacccg aatttaaggc
     1921 agaataactg ccactaatgg gcgaaacagg cacattgaat aaatcttggt aatacgcctc
     1981 cgtttttaca cgccaatacg aagaaattgt ccaatcgtaa ccgcccacaa tgtgatgcga
     2041 tcgcgtgtaa ccagtagtca tattattgcg agcatacgta tcggtatctt tgtaatacgc
     2101 ctcataaaaa tatatggtac gagcttgtat ctgactgtgc aatccgtaac ccaaagagaa
     2161 agcgtgttgc tgatgagctt gccaacgtag agcaaggcga ggttcaatgg cactttcgtt
     2221 tgttaaatta tacaattgat aatgcaagcc tgtattgagc gacaattcgt tggtaaactt
     2281 atgttgccaa ttggtatacg cattgtacaa gatgcttggt tctttgtcaa tagctgttag
     2341 ggtaatgcgt ttattcaaac tgttaacaaa taccgaatca aaaaaacttg atacaaaaag
     2401 actagcagaa gcacccaagg taaggttgtt ttttgcgttt atcctgcgtt taaattgtgt
     2461 gtttgccatc cacttatttt ctttgtaatt ggcggcatat aggttgaaat attttttgtc
     2521 ggtatagttt atcgagtcag acagcgtggt agaagtagcg tgctcgaaag aaagactgct
     2581 ttttaaacgg gaatgttcgt tgaacatata cgtgtgggtt aacatcgcaa tgttcaatcc
     2641 tgcaccaaaa tcggtagcat atcccatttg attgtgggta atggcttcat ctatatcaaa
     2701 agagcgtccc atcgaaataa aactattgcc tagtaaacca atcagcttaa agcgtccaat
     2761 tttgcttgtt ggcaaatcaa tcatcaccga taagtcgttg tattcgggca cagtacctcc
     2821 tgtacctata tcaaaaccca acaggctcat tagttgcaag gtagaaaaac gataatcggc
     2881 aacaaacgaa ccatttttaa taaaattacc aatttttatg ggaccctcga gtagcaactc
     2941 aaaaccatta aagcctactt gtcccattcc ttcaaaacgt tcggtattac ctgtgcgtag
     3001 gtttaaatcg aatacacccg acaaggcatt gccatattca gcactaaacg ccccggtcat
     3061 aaaatcagaa tttgccaata agttgctatt gagcatggta acaggtccgc cagtagtgcc
     3121 gtgtgcccca aagtggttgg ggttggatat ggctacacct tccatacgcc acaacaaacc
     3181 cgaaggcgaa ttgcccctga tgataatatc attgcgtgaa tcgttgccta ctaccacacc
     3241 tgcataattg gctaccatac gggcaggatc gcccaaactg ccggcaaagc gttctgtttc
     3301 ttctacacta aacgaacgtg aactcaccaa cgccaagtcg ttaataggtt tgtttttaga
     3361 aaaattggct gtaaccacta catctttgag cgaggtaatt ttctcttcta aggctatttg
     3421 caggagggtt tcttttcctg atattaccat gatattgctc atactagcag cgtgatagcc
     3481 caaggcacta acgataaaag tagaacgacc caaaggcaca ttgttcaact caaattttcc
     3541 attttcatcg gcaatggtac cttgcaatgg attagaatct gccacaacaa tacttgcacc
     3601 aatcagtggt tgaccagaaa aagcatctgt tatcacgcct cttacggttt gacccgtttc
     3661 agagaatagt gctgcttgaa ttaaaaagaa cgtagaaata agtaagaaca actttttcat
     3721 gctaattgct ttaattttgg cacaaaaata atgaataaat gggaatcatg cgtacataga
     3781 aaggttgttc gtgttttatt cacaaaaaat taatatgaaa gcaatgctat atgtttgact
     3841 attttttgtt gtatttttca aagtaattat ctatctctgg tcttaattct aagccaattc
     3901 tttcaaaaat atccagtaag tctgtatatg ctccttgccc acctaaaaaa tcccaaaatt
     3961 ctgctgccac ttttaattca ttatccaaat caagcatacc tgccattgtc cagcgagaat
     4021 atggctttgg ttcataggga ttatatggga ttgcgattaa tgaatttaca atagccttag
     4081 gatcattagc aagcacacaa gctgtccatt ctagcaaagt ccttttaaac tctttaaaac
     4141 cacctttgtt aggttttgca gtttttatgt caaaaagaaa aagttcatca tcatgggatt
     4201 taagccaaac gtctactttt gttggtttaa cagttctcat tttaccagat tgacacactt
     4261 cccttatttt ttcaatttca ttagatttta cgggattaat tgtcgccgcc gttaaatcat
     4321 ccataatatc ttgaataact ctttgagctg cttcgctaat tcgagttcct gc
//
LOCUS       DAOPUR010000060         4366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_20236_length_4366_cov_3.463929, whole genome shotgun sequence.
ACCESSION   DAOPUR010000060 DAOPUR010000000
VERSION     DAOPUR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20236_length_4366_cov_3.463929"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PKN90_01640"
     CDS             complement(<1..197)
                     /locus_tag="PKN90_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85015.1"
                     /translation="MKQILHHSNNLLIALSVGLLFSLQSCGEKSVYNEYNKIATVGWD
                     FGTPVRFETEINDTLSAYDIT"
     gene            complement(217..1323)
                     /gene="ricT"
                     /locus_tag="PKN90_01645"
                     /pseudo
     CDS             complement(217..1323)
                     /gene="ricT"
                     /locus_tag="PKN90_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            complement(1336..2466)
                     /locus_tag="PKN90_01650"
     CDS             complement(1336..2466)
                     /locus_tag="PKN90_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNZ85016.1"
                     /translation="MQFSQIIGQEIIKQQLITSVREGRIPHAQLFLGQEGVGKLALAI
                     AYAQYVSCLNKGEHDSCGTCSSCIKYEKLIHPDLHMVFPFAKDGKTKEICDDLLPEWR
                     EFVTKNVYFSLEQWMDNIGAGNKQATIYANESNEILRKLQLTTYESAYKIMIIWLPEK
                     MNITCANKLLKILEEPPTKTLFILVSEQVEKIISTIISRTQMIKIPLIETHTMSSYLQ
                     AKHSISKENSINIARIANGNYIKALSIIKATEESQLNFTRYQEIMRYAYKNDVLATKK
                     WAEEMTSSSMGREKQKSFLDYAQHITRECFIKNIEINELNYLIDYEENFSSGFARFVN
                     EKNIQALMDFFADAATDIEQNVQGKFVFFDLGLKLMTQIKQN"
     assembly_gap    2514..2523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2740..3942
                     /locus_tag="PKN90_01655"
     CDS             2740..3942
                     /locus_tag="PKN90_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNZ85017.1"
                     /translation="MHTTKQITNNIYYVGVNDRTKHLFESLWEIPAGVSYNSYLIVDE
                     KVALVDTVDACYSDLFLSKIEGILGEKPIDYLIINHMEPDHSASIATIKRKYPSIQIV
                     GNAKTFGMLDGFFNIKDNLYEIKEADTLTLGKTVLRFYLTPMVHWVETMMTFDETNKV
                     LFSGDAFGCFGALDGGVLDSQINVDKFWPEMERYYSNIVGKYGCPVQRALAKLSNLDI
                     QTICSTHGPVWQKYLQKTISIYNDLSLYQGKKGVVVMYGSMYGNTEQMAEVIADTLVA
                     NGIENVVMHNVSKSTPSYIIADIFRYQGLIIGSPTYSNELFPLVESMLSKIKNRDIKN
                     RYFAYFGSFSWAGVANKKLAAFAEQVGFEVVYEGGVEQKFALKHDKYVECVKLAESMA
                     QRLNNSSL"
     gene            complement(4024..>4366)
                     /gene="ssb"
                     /locus_tag="PKN90_01660"
     CDS             complement(4024..>4366)
                     /gene="ssb"
                     /locus_tag="PKN90_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ85018.1"
                     /translation="EKGYKMNNGTVVPDRTEWHNIVMWRGLAEIAEKFVKKGSQLYIE
                     GKIQSRTWDDKNGVKHYATEILCENMQMLSKKSEAESGNAPLNDKSVSTKAEETPFGN
                     SDNIADDDLPF"
BASE COUNT         1301 a          734 c          725 g         1596 t
ORIGIN      
        1 atcgtaatgt catatgctga cagtgtatcg ttgatttcag tttcaaagcg aacaggggtg
       61 ccaaaatccc atccaacagt agctatttta ttgtactcat tatacactga tttttcccca
      121 caactttgta aagaaaaaag cagccccacc gataaggcaa ttagcagatt attgctgtga
      181 tgaagtattt gtttcatttg tagatgcatt tttatgtttt ctacgtttat tatttcgttt
      241 ttttgatttt ttgttatcaa aacgggttaa acttccttct tctaagtcag ttgcaaaact
      301 tttagtttct tcttcctctt tctttgtttc tttgatattg ccttgaatag caggacgtac
      361 tcctttttta tttagagcaa ttatttcctt tactttatcc agagataatt caactacatt
      421 ttttggaaaa tttggagctt gagaatatgc catctttttt tcaaaaatat ctaatttaaa
      481 gaaataatag gtgcaatctt ctccttctag ttgaatgtcc cttgaaggaa attcttttaa
      541 agcttctgta taggtagaaa gttcgtaatt taagcaacat tttagtttag cacattgtcc
      601 tgccaatttt tgtggattta atgataaatc ttgtacacgt gcagcatgtg tgccaacgga
      661 tgaaaaacta tttaaaaaag tagaacaaca cagctctcta ccacacggac ctgtacctcc
      721 aatacgtcct gcttcttgac gagctccaat ttgtttcatt tcgatacgca cttgaaatgt
      781 ttcagccaat accttgataa gttgacgaaa atcaacgcgt tgttctgcaa tataataaaa
      841 tattgctttg ttcccatctc cttgatattc cacatcgcca attttcatat ctagtttaag
      901 atcttgtgca atttgacgag attttaacat ggcatcgtgt tcttttgcct tagattcttc
      961 ccacttttcc aaatcagttt gctttgcctt acgataaacc cgtcttactt caatggtttt
     1021 agggtcaatg ccattctttc gcatttgtct gtctactaac aagccaacta gggttactgt
     1081 tcctatgtcg tgtccagggg ttgattctac agcaacaata tctccttttt ctagttttat
     1141 attggtcgaa ttaagataat atccttttct cgtgtttttg aattgtactt ctacaaaatt
     1201 acaatttatc tgtgtttcgg ggatatccga aagccagtcg taaacactca gcatttgcag
     1261 agagtttttt ttgcacccgc acactttaat gccacaggtt gatttcgcta atttatattc
     1321 cataagtttg tttatttagt tctgtttgat ttgagtcatt aattttaatc ccaaatcaaa
     1381 aaatacaaat ttcccttgta cgttttgctc aatatcggtt gctgcatctg caaaaaaatc
     1441 catcaatgct tgaatatttt tttcgtttac aaatcgtgca aaaccagaag aaaaattttc
     1501 ttcataatca attaaataat ttagttcgtt tatttctata tttttaataa aacattcgcg
     1561 tgtaatgtgt tgagcataat cgagaaaact tttttgtttt tccctaccca tagaggacga
     1621 tgtcatttct tctgcccatt tttttgttgc tagcacatca tttttataag catagcgcat
     1681 tatttcttga tatcgagtaa agttaagttg cgattcttcc gttgctttga tgatcgaaag
     1741 ggctttaata taattgccat ttgcaattcg agcaatattg atagaatttt ctttcgaaat
     1801 agagtgcttt gcctgtaagt acgaactcat tgtatgtgtc tcaatcaacg gtattttaat
     1861 catctgcgta cgagatataa tggtagaaat aattttttct acttgttcac ttaccaaaat
     1921 aaataatgtt tttgtcggag gttcttcgag tatttttagt aatttattag cacacgtaat
     1981 gttcattttt tctggcaacc aaataatcat tattttatat gcagactcgt atgtggttaa
     2041 ttggagctta cgtagtattt cattgctttc gtttgcataa atagtagctt gtttatttcc
     2101 tgctccaata ttatccatcc actgttctag gctaaaataa acatttttcg ttacaaattc
     2161 tctccattcg gggagtaaat catcgcaaat ttcttttgtt ttaccatctt ttgcaaaagg
     2221 gaataccatg tgtaaatcag gatgaatgag tttttcatac tttatgcatg atgaacatgt
     2281 gccacaagaa tcgtgttctc ccttatttaa acaagacaca tattgggcat atgcaatagc
     2341 taatgctagc ttcccaactc cttcttgccc caaaaataat tgtgcatgag gtattctccc
     2401 ttcacgtaca ctcgtaataa gttgttgttt aattatttct tgtccaataa tttgcgaaaa
     2461 ttgcatcgaa ctatttttgt tgttataaga tgatctcgaa acctatcttt gttnnnnnnn
     2521 nnnttactgt aaaaaaatag aatcttatca agagataaga actaacttac aaagatatgt
     2581 aaaatacatc tcttttacaa tacacaaggg ttaaacactc ttaaatatca gaatgtcata
     2641 taaaaaatgt tatatcgtgg ctatcttcaa ttttatcaag tagttgtgta gcatattttt
     2701 tttatctttg cactttaata tacatataaa aattttgtta tgcatactac gaaacaaatt
     2761 acaaacaata tttattatgt tggtgtaaac gacagaacaa agcatttatt cgaaagttta
     2821 tgggagattc ccgcaggcgt atcatataat tcatacctaa ttgtagacga aaaggttgcg
     2881 ttagtagata cggtggatgc ttgttattct gatttattcc tgtcaaaaat agagggtatt
     2941 ttgggcgaaa aacccattga ctacctcatt attaatcaca tggagccaga tcattctgct
     3001 tctattgcaa cgattaagag aaaatatcct tccattcaaa ttgtagggaa tgccaaaaca
     3061 tttggcatgt tagatggctt ttttaatatt aaagataatt tgtacgaaat aaaagaagca
     3121 gataccttaa cattgggaaa aactgttttg cgtttttacc ttacacccat ggtgcattgg
     3181 gtagaaacca tgatgacgtt tgatgaaacg aacaaagtat tgttttctgg agatgctttt
     3241 ggatgttttg gtgcgttgga cggaggggta cttgattcgc aaattaatgt ggataaattt
     3301 tggccagaaa tggagcgata ttactccaat attgttggta aatatggttg tccagtgcaa
     3361 cgtgcattag ctaaactaag taacttagat attcaaacta tatgtagtac acatggtcct
     3421 gtttggcaaa aatatctaca aaaaacgata tctatttata atgatttaag tttgtatcaa
     3481 ggaaaaaaag gagtggtggt gatgtatgga agtatgtatg gaaatacaga gcaaatggct
     3541 gaagttattg ctgatacttt agttgccaat ggtattgaga atgtggtaat gcataatgtc
     3601 tcaaaatcaa cgccctcgta tattattgca gatattttta ggtatcaagg cttgattatt
     3661 ggtagtccta catactcaaa cgaactattc cctttggtgg aatcaatgtt gagtaaaatt
     3721 aaaaatagag atattaaaaa cagatatttt gcttattttg ggtcgttttc gtgggcaggt
     3781 gttgccaata agaagctcgc tgcatttgcc gaacaagttg gttttgaggt agtatacgaa
     3841 ggtggagtag aacaaaaatt tgcattaaag cacgataaat acgtggaatg cgtaaaattg
     3901 gctgaaagta tggctcaacg attgaacaat agttcattat aaaattatct caaaaagagt
     3961 tgataaaaaa agacagaaca aataattgct ctgtcttttt ttttaggcta gtgaatgact
     4021 gaattaaaat ggcaaatcgt catctgcgat gttgtcgcta ttgccaaaag gggtttcctc
     4081 tgctttggtg cttacagatt tgtcgtttaa aggagcattt ccactttctg cttctgattt
     4141 tttgcttaac atttgcatat tttcgcacaa aatttctgtt gcataatgtt ttacaccatt
     4201 cttatcatcc catgttctac tttgaatttt tccttcaatg tataattgag agcctttttt
     4261 tacaaatttt tcagcaattt cagctaatcc tctccacata acaatattat gccactctgt
     4321 tctatcaggc acaacggtgc cattattcat cttataccct ttttct
//
LOCUS       DAOPUR010000061         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_20268_length_4361_cov_3.529726, whole genome shotgun sequence.
ACCESSION   DAOPUR010000061 DAOPUR010000000
VERSION     DAOPUR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20268_length_4361_cov_3.529726"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKN90_01665"
     CDS             complement(<1..509)
                     /locus_tag="PKN90_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNZ85019.1"
                     /translation="MSHHHHHHSPIVESSFSLGKAFFFAIVLNVLFVVAEFATGFIYN
                     SIGLISDAAHNLGDVASLLLAWLAFKLAKVSTKTNYTYGFKKSTILVALTNAIILLFT
                     VGVIIVKSISKFYYPSPVEGGVIAWVAGVGVVINLATAFLFIRDKEKDLNVKGAYLHM
                     MADAIVSVGV"
     gene            complement(533..709)
                     /locus_tag="PKN90_01670"
     CDS             complement(533..709)
                     /locus_tag="PKN90_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ85020.1"
                     /translation="MDSVAKVLDVIKKASEPVNATKVVELSGLDKKEVDKAMKTLKDA
                     GHIVSPKRCYWSAK"
     gene            complement(857..>4361)
                     /gene="nifJ"
                     /locus_tag="PKN90_01675"
     CDS             complement(857..>4361)
                     /gene="nifJ"
                     /locus_tag="PKN90_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNZ85021.1"
                     /translation="QAAAHIAYMFSEVAAIYPITPSSTMAEYVDEWAANGRKNIFGEP
                     VQVQEMQSEAGAAAAMHGALQAGALTTTFTASQGLLLMIPNMYKVAGELLPGVYHVSA
                     RALASHALSIFGDHQDVMAVRQTGCALLATGSVQEVMDLAGVAHLAAIKTRVPFVHFF
                     DGFRTSHEIQKVEQLDQEDLIPLIDQEALQAFRKRALNPESPVLRGTAQNPDIYFQAR
                     EACNPFYDAIPAVVEDYMAQLSAITGRNYGLFDYYGSQDAERIIVAMGSATEAIREGI
                     DYLVAKGEKVGLISVHLYRPFSAKHFLSVMPSTVKRIAVLDRTKEPGAGGEPLYLDVK
                     DVLYGQKNQPLVVGGRYGLSSKDFTPSQVFAIYENLALPEPKNHFTVGIVDDVTFTSL
                     PMKEEFSLADAGTYEAKFYGLGADGTVGANKNSIKIIGDNTNKYCQAYFAYDSKKSGG
                     FTCSHLRFGDTPIRSTYLVNTPDFVACHVQAYLKLYDVTKGLKKNGTFLLNTVWTPEE
                     VKKNLPDNVKKYLAKNNITLYIIDATNIALELGLGNRTNTILQSAFFKITNVIPYELA
                     VEKLKYAINKSYGKKGEKIVNMNYAAVDKGGEYVKVDVPAEWANIALESDLSTTTVPE
                     FIQKVVRPINAQDGDSLPVSSFTGREDGTWNQGTAKYEKRGVAASVPVWDAENCIQCN
                     QCAYVCPHAAIRPFVLDEAEQAKAPFKEMLKANGKQFAGMAFRIQVDVLDCMGCNNCV
                     DVCPGNKGNKALELVAIETQYPNQDNWDFCMNEVSSKQHLVDVKANVKNSQLATPLFE
                     SSGACAGCGETPYVKLVTQLYGERQMVANATGCSSIYSASAPSTPYTTTPEGRGPAWA
                     NSLFEDNAEFGLGMVFAEEHMRARIVRIMNEAIASDCCPNEIKDLFKEWLENRLDEQK
                     SKELQKQITPLVKASTCSHCQQIATMTQYLVKQSQWIIGGDGWAYDIGFGGLDHVIAS
                     GKNVNILVLDTEIYSNTGGQASKSTPVGAVAKFAASGKKVRKKDLGLIAATYGYVYVA
                     QVAMGANQAQTLKAIREAEAYDGPSLVIAYSPCISHGIAAGMGKAQEEQKRAVECGYW
                     HLYRYNPDMELEGKNPMQLDSKEPDWSKFQDFLKGEVRYTSLLKQFPQEAKELFEAAE
                     NNAKWRYNSYKRMANQQWNVE"
BASE COUNT         1333 a          918 c          648 g         1462 t
ORIGIN      
        1 accccaacag atacaatagc atccgccatc atatgcaaat aagctccctt tacattcaaa
       61 tctttttcct tatcgcgaat aaaaagaaaa gctgttgcta aattaataac aacccctact
      121 ccagcaaccc aggcaatcac acctccttct acaggacttg gataataaaa ttttgaaatg
      181 cttttaacaa taataactcc caccgtaaac aacaaaatta tagcattcgt aagtgccacg
      241 agtatggtgc tttttttaaa accatatgta tagtttgttt ttgttgacac ctttgccaat
      301 ttaaatgcca accaagctaa caacaaacta gccacatctc ccaaattatg tgctgcatct
      361 gatattaatc ctattgaatt atagataaaa ccagtggcaa attcagcaac tacaaataat
      421 acattcagca caatggcaaa aaagaaagct ttccctaacg aaaaagaaga ctctactatt
      481 ggtgaatgat gatggtggtg gtgagacata ctattgtaat gttatgattg agctatttag
      541 cactccaata acaacgcttt ggagacacga tatgcccagc atcttttaat gtcttcattg
      601 ccttatctac ttctttctta tctaaaccag ataattctac tacttttgtt gcatttacag
      661 gttctgatgc tttttttatc acatctaata cttttgctac tgaatccata ttctatctat
      721 ttttaaatta ataaataatc aaaattacaa aaatatatgt agtagctatg tttattttta
      781 gatattttaa taaaaaagga gagtatatgt tttttactcc atatactctc cttgcatttt
      841 acaatcattt ctctagttac tcaacattcc attgttgatt tgccatacgt ttatatgaat
      901 tataacgcca tttagcatta ttttcagcag cctcaaatag ttcttttgct tcctgaggga
      961 attgtttcaa caatgatgtg taacgaacct ctccttttaa gaaatcttgg aatttgctcc
     1021 aatctggttc tttagaatct aattgcattg ggtttttgcc ctcaagttcc atatctggat
     1081 tatatcgata taagtgccag taaccacatt caacagcacg tttttgctct tcttgagctt
     1141 tgcccatacc tgcagcaata ccgtgactaa tacatggaga atacgcaatt acaagagatg
     1201 gtccatcata tgcctctgcc tctctaatag ctttcaaggt ttgtgcctga ttagctccca
     1261 tggctacttg tgctacatac acataaccat aagtggctgc tattaaacct aaatcttttt
     1321 tacgtacttt tttacctgat gcagcaaatt tagctaccgc tccaactgga gttgatttag
     1381 atgcttgtcc accagtatta gagtaaattt cggtatcaag caccaaaatg ttaacatttt
     1441 ttcctgatgc aatcacgtga tctaatccac caaaaccgat atcgtaagcc caaccatcac
     1501 cacctataat ccattgcgat tgtttaacta aatactgtgt cattgtagca atttgttgac
     1561 aatgagaaca tgtacttgct ttaactaatg gagttatttg tttttgtaat tctttagact
     1621 tttgttcatc taatctattt tcaagccatt ctttaaataa atctttaatt tcatttggac
     1681 aacaatcgct agctatcgct tcattcataa tacgtacgat gcgtgcacgc atatgttctt
     1741 ctgcaaacac catacctaaa ccaaattcag cattatcttc aaacaatgaa tttgcccatg
     1801 ctggacctct tccttctggg gttgttgtat aaggagtaga aggcgctgat gcagaataaa
     1861 tagagctaca acctgtagca tttgccacca tttgtctttc tccatacaat tgtgttacca
     1921 attttacata aggagtttcg ccacaaccag cacaagctcc agatgattca aataaaggag
     1981 ttgctaattg tgaattcttt acgttggctt ttacatccac caaatgttgt ttcgaactta
     2041 cttcgttcat acagaaatcc caattgtcct gatttggata ttgtgtttca atcgctacca
     2101 attcaagagc cttgtttcct ttatttcctg ggcacacatc cacacagtta ttacatccca
     2161 tacagtctaa tacatcaact tgtatacgaa atgccatacc tgcaaattgc ttaccatttg
     2221 ctttaagcat ctctttaaat ggtgctttag cttgctctgc ttcatctaac acaaacggac
     2281 gaatagcggc atgaggacac acataagcac attgattaca ttgaatacaa ttttccgcat
     2341 cccatacagg tacagaggct gccacaccac gtttttcgta ttttgctgta ccttgattcc
     2401 atgtaccatc ttctcttcct gtaaatgacg aaacgggaag actatctcca tcttgtgcat
     2461 taattggacg aacaactttt tgaataaatt ctggcacagt agtagtagac aagtcacttt
     2521 ccaaagcaat atttgcccac tcagcaggaa catctacttt tacatattca ccacctttat
     2581 ccacggcagc ataattcata tttacaattt tttctccttt ttttccatat gacttgttaa
     2641 tagcatattt taatttttct accgctaatt cataaggaat cacattggta attttaaaga
     2701 aagccgattg taaaatagta ttagtcctat tgcctaaacc aagttctaaa gcaatatttg
     2761 tagcatcaat aatatacaaa gtaatgttat ttttagctaa atattttttt acattatcag
     2821 gtaaattctt tttaacctct tctggtgtcc aaactgtatt caacaaaaat gtcccattct
     2881 ttttcaaccc tttggttaca tcatacaatt ttaaataagc ttgcacgtga catgctacaa
     2941 agtctggtgt atttactaaa taagtagaac ggataggtgt atctccaaaa cgaagatgag
     3001 aacaagtaaa acctcctgat tttttagagt cgtatgcaaa atatgcttga caatatttat
     3061 ttgtattatc tccaataatt ttgatagaat ttttatttgc acctaccgta ccgtctgctc
     3121 ctaatccata aaatttagct tcgtaagtac cagcgtctgc caatgaaaat tcttctttca
     3181 ttggtaaaga tgtaaaggtt acatcatcta caatacctac tgtaaagtga ttcttaggtt
     3241 ctggcaatgc caaattttca taaatagcaa atacttgtga tggagtaaaa tctttagacg
     3301 acaatccgta tctacctcct acaacaagag gttgattttt ttgtccatac aatacatctt
     3361 tcacatctaa atacaacggt tctcctccag ctccaggttc ttttgttctg tcaagtacag
     3421 caattctttt tactgtgctt ggcattaccg atagaaaatg tttagcagag aatggacgat
     3481 ataaatgcac tgaaataaga cctaccttct ctccttttgc tactaaataa tcaattcctt
     3541 cgcgaatagc ctctgttgct gaacccattg ctacaataat tctctcagca tcttgtgaac
     3601 cataataatc aaataatcca taattacgtc cagttattgc tgaaagttgt gccatataat
     3661 cttctaccac agcaggaatt gcatcataaa aaggattaca agcctcacgt gcttgaaaat
     3721 aaatatctgg attttgagca gtaccacgta atactggact ttctggattt aacgctcttt
     3781 tacggaatgc ttgtaatgct tcttgatcaa ttaatggaat taaatcttct tggtctaatt
     3841 gttctacttt ttgaatttca tgtgatgttc taaatccatc aaaaaaatgg acaaaaggaa
     3901 cacgtgtttt tattgctgcc aaatgagcta ctccagccaa atccataacc tcttgaacag
     3961 atccagttgc aagcaaagca catcctgttt gacgaacagc cataacatct tgatggtctc
     4021 caaaaattga taaagcatgt gatgctagtg cacgagcaga cacgtgatat actcctggca
     4081 ataattcacc agcaacttta tacatattag gaatcatcaa caataaccct tgtgatgccg
     4141 taaatgtagt ggttaaagcc cctgcttgta atgccccgtg catagctgct gctgctccag
     4201 cttctgattg catttcttgt acttgtacag gttctccaaa aatatttttg cgtccatttg
     4261 ctgcccactc atctacgtat tctgccatag ttgaggatgg cgtgattggg taaatagcag
     4321 ctacttcact aaacatgtac gcaatgtgtg cggcagcttg a
//
LOCUS       DAOPUR010000062         4298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_20647_length_4298_cov_3.342918, whole genome shotgun sequence.
ACCESSION   DAOPUR010000062 DAOPUR010000000
VERSION     DAOPUR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20647_length_4298_cov_3.342918"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKN90_01680"
     CDS             <1..345
                     /locus_tag="PKN90_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85022.1"
                     /translation="DEIVVGMGYKISDFDVILRLQSNQQKKVKNDLNLRADFSIRDNK
                     TIVRKIEEAYAQATSGNMILSVKFLADYVFSERINISFYYDLQANNPVVMTSYPISNS
                     NVGFSVKMLLTR"
     gene            complement(350..1429)
                     /locus_tag="PKN90_01685"
     CDS             complement(350..1429)
                     /locus_tag="PKN90_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNZ85023.1"
                     /translation="MLKKLDIYIIKKFLGAYFLSIILIMSIAIVIDLTEKLDNFYATN
                     LELSTIIFDYYINFIPYYANLFSSLFTFISVIFVTSKMAFNSEIIAMLASGISLKRIL
                     FPYFIAAGIIALITFFIGGYIIPPGNKVRYEFEQKYIKKKPAEKTIRNIQLKVSTNQI
                     AYIERFELEDNTGYRFSLDTFEGKTLVSRTTSNTIHWDSTYVWTLINYTKRDFVGLKE
                     ELSMGDKTTITINMKPSDFGGIKNQYEHLSNPALKAHIEEQTQRGTAGLNLYKLEYEK
                     RYALPFAAFVLTLIGVSLGSRKVRGGTGLHILIGFMLSITYVLSTMISSSLTVKAGFN
                     PMVAIWSPNVVYFLIGLYLYKIAPK"
     gene            1596..1700
                     /locus_tag="PKN90_01690"
                     /pseudo
     CDS             1596..1700
                     /locus_tag="PKN90_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
     gene            1796..2557
                     /locus_tag="PKN90_01695"
     CDS             1796..2557
                     /locus_tag="PKN90_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNZ85024.1"
                     /translation="MLFSCSSVKKQATGESNAILPDVHTSRVALDWQGVYRGMLPCAS
                     CEGIKTQLELFDSTYVLSTQYSGKGDSIYVSNGRFIWSQSGNSLVLIDTLFTDSLHYK
                     VAENQVIQLDKDGNEIKSGLRNMYVLQKDTEQLVDKMWQLTALQGKQLTANYSVFPQP
                     YIIFYADNKVGGSGGCNRFFGNYMLSATSHIGFSAIGSTRRACFESMDVENEFLSIFH
                     QIDTYSLKQDTLHLLSKEGDILATFHYHYFSNLMQ"
     gene            2563..3186
                     /locus_tag="PKN90_01700"
     CDS             2563..3186
                     /locus_tag="PKN90_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNZ85025.1"
                     /translation="MKKISLLFILSLFSVVAFSQFTLGPKVGYLTQTLSVKQEDIKAS
                     LQDIAIFGLFMRIGNSVYVQPEINYFTSGAVFKRPQQGSVNPIEQEVYLQNVQIPFFI
                     GTKLINTRLFNLRAMMGPTANIVVEKRINTQESGTYINPIKEADIKDLYWGLQVGGGM
                     DIAFVTLDVQYIIGLSSIIGDVEIEGNPVLFDSKNQGFVVTLGLKLF"
     gene            complement(3222..3674)
                     /locus_tag="PKN90_01705"
     CDS             complement(3222..3674)
                     /locus_tag="PKN90_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ85026.1"
                     /translation="MSYEQLKLSNQLCFPMYAASRLITREYQPFLDALGITYPQYLVL
                     MVLWEHGEKSVNEIANTLLLNTNTITPLLKRMEKIGLIERERSLQDERKILVCVSDKG
                     KELEEKAASIPIQLIQNLGKSKLSINDLMTLRNQLNDLIDFLQNKKSK"
     gene            complement(3707..4195)
                     /locus_tag="PKN90_01710"
     CDS             complement(3707..4195)
                     /locus_tag="PKN90_01710"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNZ85027.1"
                     /translation="MATQLGFYDFTAKSLQGKETSLATYAGKTIIIVNTASKCGLTPQ
                     FEGLENLYQEYKDKGLVILGFPCNQFANQEPGDEKSISEGCLLNYGVTFPMFAKVDVN
                     GSHAHPIYVYLKKELPGFLGVKGIKWNFTKFVIDKNGNPVKRFSPYDTPEKMKAYIEK
                     IL"
BASE COUNT         1352 a          660 c          790 g         1496 t
ORIGIN      
        1 gatgaaattg tggtaggaat gggatataaa attagtgatt ttgatgtgat attacgtttg
       61 caatcaaatc aacaaaaaaa ggtgaaaaat gacctcaatt tacgtgcaga tttctctatt
      121 cgagataata aaactattgt acgaaaaatt gaagaagcat atgctcaggc tactagcggt
      181 aacatgattt tgagcgttaa gtttttggca gactatgtat ttagtgagcg tataaatatc
      241 agtttttact acgatttaca ggctaataat cctgtggtta tgacatcata tcctatctcc
      301 aattccaatg taggatttag tgtcaagatg cttctaacta gataaaaaat tactttgggg
      361 ctattttata aaggtataat ccaattaaaa aatagacaac attgggcgac catatggcta
      421 ccattggatt aaatcctgct tttactgtta atgatgacga tatcattgtt gataatacat
      481 acgttatact taacataaac ccaattaaaa tgtgtaagcc tgtacctcct ctaactttgc
      541 gagaccccaa tgatacacca attagagtta atacaaaagc tgcaaatggt agggcataac
      601 gtttttcgta ttcaagctta tacaggttga gacctgctgt tcctcgttgt gtttgttctt
      661 caatatgtgc ttttaaggca ggattgctaa ggtgttcgta ttgatttttt atccctccaa
      721 aatcagatgg tttcatatta attgtaattg ttgttttatc tcccatagac aattcttcct
      781 ttaatccaac aaaatcacgc ttagtgtaat ttatcagcgt ccatacatat gttgagtccc
      841 aatggatggt gttagaagtt gtgcgtgata ctaatgtttt tccttcaaaa gtgtctaacg
      901 aaaacctgta tcctgtgtta tcttctaatt caaaacgttc aatataggca atttggtttg
      961 tggacacttt taactggatg tttcggatgg ttttttcagc tggttttttt ttaatatatt
     1021 tctgttcaaa ttcgtaacgc actttgttac caggaggaat aatatagcct ccgataaaga
     1081 aagtaatcag tgcgataatg cctgctgcaa taaaataagg gaataaaata cgtttcaagc
     1141 ttataccgct tgcaagcata gcaataattt ctgaattgaa agccattttg gaagtaacaa
     1201 atataactga aataaatgta aataaggaac taaataaatt ggcgtaataa ggaataaagt
     1261 taatgtagta gtcaaaaata atagtagata attcaagatt tgtagcataa aaattgtcta
     1321 atttttcggt taaatcaatg acaatggcga tgctcattat aagtatgata gatagaaaat
     1381 aggctcccaa aaatttttta ataatatata tatcaagttt ttttaacatg gctatggttt
     1441 tataaattaa attgcggtgc aaaaatacga aaaattaact gaaatagata gaatgcttat
     1501 ttttaataaa aaaaatgaga atatttcgtt tattgcaaag tttgttgtat ttttgaaact
     1561 aattaaataa gtatgaattt ttaaaagaaa catgtatgaa aacattcgta tgttcagtat
     1621 gtgggtatgt tcaccaaggc gatacacccc cagaaaagtg tcctaaatgc aagcaaccag
     1681 caaaagtatt taaggaagtg aaataatttt tttaaaacta tccctaaaaa gatagttttt
     1741 ttcacattac tagataatga tgagaaaata ttttttatta gtaagtgtcg ttgttgtgtt
     1801 attttcctgt tctagtgtaa aaaagcaggc aactggcgaa agtaatgcga ttttacccga
     1861 tgtacatacc agtcgtgttg ccctcgattg gcagggagtt tatcgtggta tgttgccttg
     1921 tgcaagttgc gaggggataa aaacacaact cgaacttttc gattctactt atgttctttc
     1981 tacacaatat agtggtaagg gtgattctat ctatgtttca aatggtcgtt ttatatggtc
     2041 tcaaagtggt aattccctcg tacttattga tacacttttt accgattcgc tgcattataa
     2101 agtagctgaa aatcaggtta ttcaattaga taaagatgga aacgagatca agtcaggatt
     2161 acgaaatatg tatgtgctac aaaaagatac agaacaatta gtggataaaa tgtggcaatt
     2221 aacagccctt caaggaaagc aattaactgc gaattattca gtattcccac agccatacat
     2281 tattttttat gcggacaaca aagtaggtgg cagtggtgga tgtaaccgtt tttttggtaa
     2341 ttatatgctt tctgctactt cacatatagg tttttccgct attggaagta cccgaagagc
     2401 atgttttgaa tcgatggatg tggaaaatga gtttttgtcc atctttcacc aaattgacac
     2461 ctatagcctg aagcaagata cgttacatct tttatcaaaa gaaggagata tactagctac
     2521 ctttcactat cattatttta gtaacttaat gcaataagtg aggtgaaaaa gattagtttg
     2581 ttgtttattt tgagtctttt ctccgttgta gccttttcac agtttacact aggtccaaag
     2641 gttggttatc ttacccaaac gttgtctgtt aagcaagaag atattaaggc aagtttacag
     2701 gatatagcca tttttggtct ttttatgcgt attggtaata gcgtgtatgt gcaaccagag
     2761 ataaattatt ttacgtcagg agctgttttc aaacgacctc aacaaggttc tgtaaatccc
     2821 attgagcaag aagtgtattt gcaaaatgtt caaattcctt tttttatagg cacaaaattg
     2881 attaacacac gcttgtttaa tttacgtgcc atgatgggac cgactgccaa tattgtggtt
     2941 gaaaagcgta tcaataccca agaatcaggt acgtatatca atcccattaa agaggcagat
     3001 atcaaagatt tgtattgggg tttgcaagta ggcggtggta tggacattgc attcgttacc
     3061 cttgatgttc aatacattat tggtttaagt tctattattg gtgatgtaga aatagaaggt
     3121 aatcctgtgt tattcgattc taaaaatcag ggatttgtag taacacttgg tctcaaattg
     3181 ttctaacata gaataggctt actcaagtgt gaagagtaag tctattttga ttttttattt
     3241 tgcaaaaaat caattaggtc gttcagttga tttctgaggg tcatcaagtc gtttattgac
     3301 agcttgcttt tacccaagtt ttgtattaat tgtataggaa tggaagctgc tttttcttct
     3361 aattcttttc ctttatcgct tacgcaaacc aatattttac gttcatcttg caacgaccgt
     3421 tctcgctcta tcaagccgat tttttccatt cttttcaaca aaggtgttat ggtgttggtg
     3481 ttgagcagta gggtattagc tatttcgttc acactttttt cgccatgttc ccatagcacc
     3541 atcagtacca agtattgtgg gtacgtaatg cccaatgcat ccaaaaatgg ttggtattcg
     3601 cgtgtaataa gccgtgaagc agcatacatc ggaaagcaca attggttgct gagttttaat
     3661 tgttcgtagc tcatggtaaa aatgtttgta caaaaatagt atttatttac aatatctttt
     3721 caatataagc tttcattttt tcgggtgtat cgtagggtga aaatcttttt acgggattcc
     3781 catttttatc aatcacaaac ttagtaaaat tccattttat ccctttaacg cccaaaaagc
     3841 ctggtaattc tttttttaga tacacataaa tagggtgagc atgtgagcca ttcacatcta
     3901 ctttggcaaa catcggaaaa gttacgccat agttgagcaa gcaaccttca gatatagatt
     3961 tttcatcgcc aggttcttga ttcgcaaatt ggttgcaagg aaagccgagt attactagcc
     4021 ccttatcttt gtattcttga tacaagtttt ctagtccttc aaattggggt gttagtccac
     4081 atttgcttgc tgtgtttact ataataattg ttttgcctgc ataagttgca aggctggttt
     4141 cttttccttg taatgattta gctgtaaaat cataaaatcc tagttgtgtt gccattatgg
     4201 ttatttatta gttataaata tatcgtatgc gatacaaatg tacggttatt attttatatc
     4261 gcaagcgatt taaaataatg taacgaaagg aagtataa
//
LOCUS       DAOPUR010000063         4271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_20826_length_4271_cov_3.373102, whole genome shotgun sequence.
ACCESSION   DAOPUR010000063 DAOPUR010000000
VERSION     DAOPUR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20826_length_4271_cov_3.373102"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKN90_01715"
     CDS             <1..278
                     /locus_tag="PKN90_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HNZ85028.1"
                     /translation="NGFPLPITTLVAQLEGTRYVTRQSVHTANAVKKAKKAIRKALEN
                     SAEGKGTSFVEFVSTCNAGWKMSPAEANTWMEKNMIPVYPIGDLKDK"
     gene            296..736
                     /locus_tag="PKN90_01720"
                     /pseudo
     CDS             296..736
                     /locus_tag="PKN90_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
     assembly_gap    737..746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(867..1181)
                     /gene="trxA"
                     /locus_tag="PKN90_01725"
     CDS             complement(867..1181)
                     /gene="trxA"
                     /locus_tag="PKN90_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNZ85029.1"
                     /translation="MAKEFTESNFEELLQNNVVVVADFWAEWCGPCRMITPIVEELAS
                     AYEGKVLIGKVNVDEEGDLATKYGIRNIPTVLFFKNGQIVDKQVGAAGKNVFEDKINA
                     LL"
     gene            complement(1255..4260)
                     /gene="dnaE"
                     /locus_tag="PKN90_01730"
     CDS             complement(1255..4260)
                     /gene="dnaE"
                     /locus_tag="PKN90_01730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNZ85030.1"
                     /translation="MRYSKQEWLKTTDEMATIFSDLPEAIENTLEIANKVENYSIDEP
                     NPIMPTFDIPIDFGTEEGYRQQYTHQDLLKEFTADEHGVMHLSEKEALEKVHRLGGID
                     RLYRIKLEADYLRKITFDGAQVRYGENIAQEVKERIEFELHVMKTMGFPGYFLIVYDF
                     IQAARKMEVTVGPGRGSAAGSVVAYCLRITDIDPLKYDLLFERFLNPDRISMPDIDID
                     FDDDGRSKVLDYVAHKYGKERVAQIITYGTMATKMAIKDVARVQKLSLPESERLTKLI
                     PTSFPEENGKSPKVTISNCIERIPELHEARYSSDARLSDTLKYAEMLEGTVRQVGIHA
                     CGVIIGADDLTNFVPISIDKNNSETLVTQYEGSTIETVGLIKMDFLGLKTLSIINETL
                     RNIKASKGITIDIDTIPIDDKKTYELYSKGLTTGTFQFESSGMQKYLRELKPNRFEDL
                     IAMNALFRPGPMAYIPSFINRKHGIEEIQYDIPIMEKRLKETYGITVYQEQVMLLSRD
                     LAGFTRGESDELRKAMGKKLQDKMDALKVKFINGATKNGYGPIELLEKIWKDWAEFAK
                     YAFNKSHATCYSWISYQTAYLKANYTADFMAANLTRNKDNITEITKFMEECKQLGIKV
                     LGPSVNESNLNFISNPAGDIRFGLGGIKGVGYGAVEAIVTERQANGRYTSVFNFLERV
                     NLNACNKRAIESLALSGAFDEFDDVKREAFFAENSKGEVVLETLMRYASRYQIDQQSS
                     QQSLFGDLESVAIQRPEIPQVQSWSNLYKLTKEKDMIGIYLSAHPLDFYEIELASLCT
                     QDVTRLKELSELHDSIGSNPVKIAGIITKVNRLISQNNQPFARFTLEDYSGSHEISVF
                     GKQFTELDNMLRENYYVLIHGVVQERQADYKFKPKPEDEARGRELVFKIQTIELLETI
                     KDKGIEELKIQIPLLNISYELIAELSALLSENVDKKAKTRFCVEVIDPETRWIVKLNK
                     KDGKITVNKPIFHFLKEKKENQILDFIIS"
BASE COUNT         1349 a          815 c          637 g         1460 t
ORIGIN      
        1 taaatggttt tcctcttcct attactactt tagtagccca attagaaggt acacgctacg
       61 taacacgaca aagtgtgcat acggcaaatg ccgtaaaaaa agcaaaaaaa gctatccgaa
      121 aagcacttga aaattcagca gaaggcaaag gaacatcatt tgtagaattt gtttctacat
      181 gcaatgctgg ttggaaaatg tcaccagctg aagccaatac atggatggaa aaaaacatga
      241 taccagttta tccaattggc gatttaaaag ataaataaca aacaacattt gacacatgaa
      301 acaagaaatt attatagcag gttttggtgg acaaggagta ttatcaatgg gaaaaattct
      361 tgcttattcg agtttacttg aggggaaaga agtaagttgg tttccatcat atggaccaga
      421 acaacgtggt ggtacagcaa atgttacagt tattgtaagt gataatcgta ttagctctcc
      481 tattcttaat acttttgatg tagcaattat cctcaaccaa caatcactaa ataaatttga
      541 aagtaaagta aaacctggag gaatacttat atacgacggg tatggtataa gcaaagaacc
      601 cactcgcact gatatattca tatatcgcat taatgcaatg gatacttcaa tagcaatgaa
      661 tgctgccaaa tcattcaata tgattatatt aggaggatta cttaaagtat tgccatttgt
      721 taatatagat atacctnnnn nnnnnnatga atgaggctgc tatacgaaaa ggtatggaga
      781 ttattgagaa aatataattt ttttaaatag aaaaagggag ttcatactaa catgaaactc
      841 cctttttttt cgaaaagata taggtattac aatagtgcgt tgattttatc ttcaaataca
      901 tttttacctg ccgcacctac ttgcttatca acaatttgac catttttaaa aaacaataca
      961 gtaggaatat ttcggatacc atatttggta gctaagtctc cttcttcatc aacattaact
     1021 ttcccaatca atacttttcc ttcgtaagca ctagcgagtt cttcaacgat aggggtaatc
     1081 atacgacagg gtccacacca ttctgcccaa aaatctgcta ctacgactac gttattttgt
     1141 aataactctt caaaatttga ttctgtaaat tcttttgcca tgtttgtcta tttttattat
     1201 tagttgatat aaatatatat gtgcaaatgt atataatttc aatgtaaaat cctattaaga
     1261 aatgataaaa tcaagtattt gattttcttt cttttcttta agaaaatgaa agattggttt
     1321 attgactgtt attttcccat cttttttatt caatttaaca atccaacgtg tttcgggatc
     1381 tataacttca acacagaaac gagtttttgc ttttttgtct acattttctg acaataaagc
     1441 agataattcg gcaattaatt catacgaaat atttaacagg ggaatctgta tttttagttc
     1501 ttctatacct ttatctttaa ttgtttccaa caattcaatt gtttgaattt taaacaccaa
     1561 ttctcgtcca cgtgcttcat cttctggttt aggtttaaac ttatagtctg cttgtcgttc
     1621 ttgtaccact ccatgtatca atacataata attttctctc aacatattgt ctagctcggt
     1681 aaattgtttg ccaaatacac taatttcatg cgatcctgaa taatcttcga gtgtaaagcg
     1741 agcaaagggt tgattatttt gagaaattaa tcggttaact ttggttataa tacctgctat
     1801 tttaacagga tttgaaccta tactatcatg caattctgat aattctttta atcgtgtaac
     1861 atcttgtgta cacaaggatg caagttcaat ttcgtaaaaa tctagaggat gagccgacaa
     1921 gtaaatgcca atcatgtctt tttccttagt tagtttatat aaatttgacc acgattgtac
     1981 ttgaggaatt tcaggtcgtt gaatagcaac agactctaaa tctccaaaca acgattgttg
     2041 agaagattgc tgatcaattt ggtatctact agcataacgc ataagggttt ctaaaacaac
     2101 ctctccttta gagttttctg caaagaaagc ttctcttttt acatcatcaa attcatcaaa
     2161 tgctcctgat aaagccaaac tttctatcgc tcttttgtta caagcattta gattcaccct
     2221 ttctaaaaaa ttaaaaacac ttgtgtacct tccatttgct tgcctttcag ttacaatagc
     2281 ttctacagca ccatacccca ctccttttat gccaccaagt ccaaaacgaa tatcgccagc
     2341 tggattagat atgaagttta aattactttc gtttacactt ggacctaaaa ctttaatacc
     2401 aagctgctta cactcttcca taaacttggt aatttcagta atattatcct tgttcctggt
     2461 aaggttcgcc gccataaaat cagccgtata gttagccttt aaataagcag tctgataaga
     2521 tatccacgaa taacaagtag catgtgattt attaaaagca tactttgcaa attcagccca
     2581 atctttccaa attttttcca acaattctat tggaccatag ccatttttgg ttgcaccatt
     2641 gataaattta acttttaagg catccatttt atcctgcaac tttttcccca tagcttttcg
     2701 caattcatca gactcaccac gtgtaaatcc agctaaatct cgactaagta acatcacctg
     2761 ttcttgatac acagtaatcc catacgtttc ttttaatcgt ttttccatga tgggtatatc
     2821 atactgtatt tcctctattc catgttttct gttaataaag cttggaatgt aagccatcgg
     2881 acctggtcga aacaaagcat tcatggctat taaatcctca aaacggttgg gttttagttc
     2941 acgcaaatat ttttgcattc cgctcgattc aaactgaaat gtaccggtgg tcaatccttt
     3001 gctatacaac tcataggttt ttttatcatc tatgggtata gtatcaatat ctatagtgat
     3061 accttttgat gctttgatat tacgcaacgt ttcgttaata atagacaagg ttttcaaccc
     3121 taaaaagtcc atttttatta aaccaaccgt ttcaatagtt gatccttcat attgtgtaac
     3181 taaagtttcg gaattatttt tatcaatact aataggaaca aaattggtta aatcatcagc
     3241 accaataata acaccacatg catgtatgcc tacttgtctt actgttcctt caagcatttc
     3301 ggcatatttt aaagtatccg ataagcgagc atctgaagaa taacgtgctt catgtaattc
     3361 aggaattcgt tctatacaat tagagatagt tactttaggc gatttgccat tttcttctgg
     3421 aaaactagtt ggaatcaact tagttaatct ttctgattcg ggaagagata atttttgcac
     3481 gcgtgccaca tctttgatag ccattttagt cgccatcgtt ccataagtaa taatctgagc
     3541 cacacgctct ttgccatatt tatgagcgac ataatcaagc acttttgatc tgccatcatc
     3601 atcaaaatca atatcaatat caggcatcga gatacgatca ggatttagaa aacgttcaaa
     3661 cagcaaatca tattttaaag gatctatatc tgtaattcgc aaacaatatg ccactacgga
     3721 acctgcagcc gaaccccttc ctggacctac tgtaacttcc atcttacgag cagcttggat
     3781 aaagtcatac acaattagaa aatatcctgg aaaacccatt gttttcatta catgaagttc
     3841 gaattcgata cgttctttca cttcttgtgc aatattttct ccgtatcgta cttgtgctcc
     3901 atcaaatgta atttttcgca aataatctgc ttctagttta atgcgataca acctatcaat
     3961 acctcccaat ctatgtactt tttccaaagc ctctttttca gaaagatgca taacaccatg
     4021 ttcatctgca gtaaactctt tcagcaaatc ttgatgtgta tattgttggc gatacccctc
     4081 ttctgttcca aaatctattg gaatatcaaa agtaggcatt attggattag gttcgtcaat
     4141 gctataattt tcaaccttgt ttgcaatttc aagcgtattt tctatggctt cgggtaaatc
     4201 tgaaaatata gtagccatct catccgtagt ctttagccac tcttgtttac tatagcgcat
     4261 acgcttttca t
//
LOCUS       DAOPUR010000064         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21125_length_4227_cov_3.060403, whole genome shotgun sequence.
ACCESSION   DAOPUR010000064 DAOPUR010000000
VERSION     DAOPUR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21125_length_4227_cov_3.060403"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKN90_01735"
     CDS             complement(<1..341)
                     /locus_tag="PKN90_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNZ85031.1"
                     /translation="MKFSITDEREVKKFFIIFYVVGIIGMAIPYTLPLFKILTPYALV
                     LSFGYLAYFHPTKKNTTSNILFFFIFLAGLVVEIIGVKTGVIFGGYEYGNSLGMKVFD
                     TPLLIGINWLF"
     gene            complement(355..1194)
                     /locus_tag="PKN90_01740"
     CDS             complement(355..1194)
                     /locus_tag="PKN90_01740"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNZ85032.1"
                     /translation="MMELYTNIAFKTSELVTKTYSTSFSIAISCLPYETKKAIYAIYG
                     FVRFADEIVDTFDKTQQKSLIKDFELDFQKALETGISMNPILHAFVLTINKYSIPLNL
                     VDSFLHSMKLDLDKQVYKTEAELREYIYGSADVVGLMCLHIFVNGDKKEYDNLLLPAK
                     RLGSAFQKVNFLRDLKADYEYLGRAYFPDFSIETFNEAKKRSIINDMETDFIEARKGI
                     SELPGKTKLAVYIAYLYFYELLKKIKKTPVHGILEARIRVSDTKKIALFAKAYIDFLL
                     HKI"
     gene            complement(1199..1738)
                     /locus_tag="PKN90_01745"
     CDS             complement(1199..1738)
                     /locus_tag="PKN90_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNZ85033.1"
                     /translation="MKRIKTFSIICSTMAFFSLTACSTIPKGVEAVTAFEQERYLGNW
                     YEIARFDFRFERNLNNTTAFYELNSDNSIKVVNRGYNYVTNEWKKATGKAKFVEKTDV
                     AMLKVSFFGPFYAGYNVIAIDKNYQYALVSGSSLKYLWILSRTPQLPQNIKEEYLQLA
                     HEKGFKVENLIWVEHNQQE"
     gene            complement(1882..2466)
                     /locus_tag="PKN90_01750"
     CDS             complement(1882..2466)
                     /locus_tag="PKN90_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85034.1"
                     /translation="METIEKKFSTENSNEVSEPNNTKSKLTFWGFIKKHPTFTTIIVA
                     LLSIGAVILYKDASHAKWQKEMTEKASNQLFNTQEDLIKLVAKPLVWNIRAEMLRNNM
                     EQVSILMTDMVRDNTIQYVHLIDTNGLVLLSTNKKLEGQIITNEFDSSLLKTTEPTTL
                     VIENIVQVAAPVMGVDRQLGVLILGYQPTMLVLE"
     gene            complement(2589..>4227)
                     /locus_tag="PKN90_01755"
     CDS             complement(2589..>4227)
                     /locus_tag="PKN90_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ85035.1"
                     /translation="EPLPSRPFPLPRLAPHLLNKIIKDGKSQQRITTTIDTKLQQYTL
                     HIGTKYATHYSHNYINNIAIIVLDTKSGDIIAYVGNVDIPGIQNASYVDNILASRSAG
                     SILKPFLYAAMLNDGDILPNSLIPDIPTRLGGYSPQNFDKKFSGTVEASKALAHSLNV
                     PAVRELNEYGLERFYDIIQELGFSTITKDPSHYGLSLILGGGEVNLYETTSIYASLAR
                     SLMSYTANNGTYTTTDFRLGNYLKDKQDIESIQSDRTIFGAGAIWCTLETLTTVNRPW
                     DETGWENFASSHKIAWKTGTSFGSRDAWSIGVTPQYTIGVWVGNATGEGRPGLTGVTH
                     AAPIMFELFKHLNPNKWFAQPNADLILLEVCSKSGYRATNICELDTIYAPKNGIKVKS
                     CPYHQLLFLDSTQTLRVSGNCYPVHKMKTRSHFVLPPIQEYYFKKLNPNYIPLPPYKE
                     NCLPEKESVIGIIEPTHNAAIYIPKGIGDQAGMVIFEAIHRNKDALLFWHIDNDYIAQ
                     TTGIHKIEVSPSVGNHILTVIDDKGNMIKRKFSILAD"
BASE COUNT         1275 a          717 c          734 g         1501 t
ORIGIN      
        1 aagaataacc aatttatacc aatcaatagg ggggtgtcaa aaaccttcat acccaaacta
       61 ttaccgtatt cgtatccacc aaaaattaca cctgttttaa cgccaataat ttcaacaact
      121 aaaccagcaa gaaaaataaa gaagaataaa atattgctcg tagtattttt tttagttgga
      181 tgaaaataag ccaaataccc aaaacttaat acaagtgcat agggagttaa aattttaaat
      241 aaaggaagtg tataggggat agccatgcca attattccta ctacataaaa tataataaag
      301 aattttttta cttcacgttc gtctgttatc gaaaatttca tatatggtaa tgggttatat
      361 tttgtgtaaa aggaaatcaa tgtatgcttt ggcaaatagt gcgatttttt tagtatccga
      421 aactcggata cgtgcttcta aaatgccatg tacgggtgtt ttttttattt ttttcaacag
      481 ttcgtaaaaa tataaatagg caatataaac agctaatttt gtttttccag gcaattcgct
      541 tattcccttg cgagcttcta taaaatcggt ttccatatcg ttgataatac tacgtttttt
      601 agcctcatta aatgtttcaa tgctaaagtc tggaaaataa gctcgaccta aatattcata
      661 atctgctttt aagtcgcgta aaaagttaac cttctgaaaa gcggagccta atcttttagc
      721 tggaagtaat agattatcat attctttttt atctccattt acaaatatgt gcaagcacat
      781 caatccaaca acatctgccg aaccataaat atattcacga agttcagctt ctgttttata
      841 tacttgcttg tcaaggtcaa gtttcatgct atgtaaaaac gaatctacta aattaagtgg
      901 tattgagtat ttgtttatag taagaacgaa ggcatgtaaa atggggttca tactaatacc
      961 tgtttccaag gctttttgaa aatctaattc aaaatctttg atgagcgact tttgttgtgt
     1021 tttatcaaaa gtatcaacta tttcgtcagc aaatcgaaca aacccataaa tagcataaat
     1081 ggctttcttt gtttcgtatg gcaagcaact aatagcgata gagaaagagg tgctataggt
     1141 tttagttacc aattcacttg ttttaaatgc aatgtttgtg tatagttcca tcatacaatt
     1201 attcctgttg attatgttct acccaaatta aattttctac tttaaagcct ttttcgtgag
     1261 caagttgtag gtattcttct ttgatgtttt gtggtagctg aggtgtgcga gataatatcc
     1321 acaagtattt aagactacta ccagatacca atgcatattg ataatttttg tcgatagcaa
     1381 tcacattata acctgcataa aaaggaccaa aaaaagaaac cttgagcatc gcaacatcag
     1441 ttttttctac aaactttgct tttccggttg cttttttcca ttcgttggtt acataattat
     1501 aaccgcggtt aacgacctta atgctattgt cggaatttaa ttcgtaaaaa gcagttgtat
     1561 tgttgagatt acgctcaaat cggaagtcaa aacgtgctat ctcataccaa tttcctaggt
     1621 atcgttcttg ctcaaaagct gttactgctt ctactccttt gggtatagtg ctacaagctg
     1681 ttaagctaaa aaaagccatt gtactacaaa taatgctgaa ggtttttatt cttttcatat
     1741 ccgataatta tttacacaaa gataggggaa aaatgaatac tatttcaaaa gaaattcttt
     1801 gaaatgcgac agctttatgg tgtactatgt actaaaaaag gcgagaagca attctcacct
     1861 tttttagagt atattttttt gctattctaa aaccagcatg gtaggttgat aaccgagtat
     1921 taaaacacct aattgcctat caacccccat tacaggagct gctacttgta ctatattttc
     1981 tatcaccaaa gttgttggtt ctgtggtttt aagcaaagaa ctgtcaaatt cgttggtaat
     2041 aatttgtccc tctagttttt tattggttga taaaagaact aatccgttcg tgtcaattaa
     2101 atggacgtat tgaatagtgt tatctctcac catatctgtc attaatattg aaacttgttc
     2161 catattattg cgtagcattt ctgcccgaat attccaaact aaaggtttcg ctactagttt
     2221 tattaaatct tcttgtgtgt taaataattg attactagct ttctcagtca tttctttttg
     2281 ccacttagca tggcttgcat ctttatacaa aatcacagct ccaatgctta gtaatgcaac
     2341 aatgatagta gtaaacgtag ggtgtttttt tataaatccc caaaaagtaa gtttggattt
     2401 tgtattgtta ggttccgata cttcgttact attttctgtg gagaatttct tttcgattgt
     2461 ttccatgatg tatatttttt gtacttcata caaatatact cctttttctt gagactagaa
     2521 aatttctgta ttcaattttt taattatcat gttttattac ttagacttgt ggttatagca
     2581 attgtttgtc aatcagctaa tatactgaat tttcgtttta tcatatttcc tttgtcgtcg
     2641 attaccgtaa gtatgtgatt gcctacagaa ggcgatacct caattttgtg aatgccagtt
     2701 gtttgggcta tgtaatcgtt gtcgatatgc caaaacaata gggcatcttt attcctgtga
     2761 atagcttcaa aaataaccat acctgcttga tctccaattc ctttcggaat atatatggca
     2821 gcattatgtg tgggctctat aattccgata actgattctt tttctggtaa acaattttct
     2881 ttgtatggag gaagggggat gtagttcgga ttaagttttt tgaaataata ttcttgaatt
     2941 ggaggtaata caaaatgcga acgtgttttc attttatgca cggggtagca atttccactt
     3001 actcgtaatg tttgtgttga atcaagaaat aataattgat ggtaggggca agatttaact
     3061 tttatcccat ttttgggtgc atatatggtg tcgagttcac aaatatttgt tgctcgatat
     3121 ccgcttttac tacaaacttc cagtaaaatt aagtctgcat ttggctgagc aaaccatttg
     3181 ttaggattta ggtgcttaaa taattcaaac ataattggag cggcatgtgt aactcctgtt
     3241 aatcctggac gtccttcacc tgtagcatta cccacccaca caccaatggt gtattgagga
     3301 gtaactccaa ttgaccacgc atcgcgagag ccaaaacttg tgcctgtttt ccatgctatt
     3361 ttgtgtgaag aagcaaaatt ttcccaacct gtctcatccc aaggacggtt tacggttgta
     3421 agtgtttcta gcgtacacca tattgctcct gctccgaaaa tagttctatc cgactggatt
     3481 gattcaatat cttgcttatc tttcaaatag tttcctaagc gaaaatcagt agttgtgtat
     3541 gtgccattat ttgccgtata tgacatcaac gaacgtgcta aggatgcata aatggatgtt
     3601 gtttcatata aattcacttc acctcctcca agtattagcg ataagccata atgcgaaggg
     3661 tctttggtta ttgtgctaaa ccctaattct tgtataatgt cgtaaaatct ttctaaacca
     3721 tattcattca actctcttac agctggaacg tttaaagagt gtgctagtgc tttacttgct
     3781 tcaactgtgc cagaaaattt cttgtcaaaa ttttgagggg agtaacctcc taatctagta
     3841 ggaatatctg ggattaagga atttgggagt atgtctccat cgttcaacat cgctgcataa
     3901 agaaaaggtt tcagaatact tccagcactg cgtgatgcca atatattatc tacgtaagaa
     3961 gcattctgta tgccagggat atccacattt cctacgtacg ctattatatc tccacttttg
     4021 gtatctaata caataatggc aatattattt atgtaattgt gtgaataatg ggtagcatat
     4081 tttgtaccaa tatgtagggt gtattgttgt aatttggtgt cgatggtggt tgtaatgcgt
     4141 tgttgacttt ttccatcctt aattattttg tttaaaagat gcggagctaa tcgtggcaaa
     4201 ggaaagggac gtgagggtaa gggctcg
//
LOCUS       DAOPUR010000065         4217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21198_length_4217_cov_3.354397, whole genome shotgun sequence.
ACCESSION   DAOPUR010000065 DAOPUR010000000
VERSION     DAOPUR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21198_length_4217_cov_3.354397"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..2434
                     /locus_tag="PKN90_01760"
     CDS             128..2434
                     /locus_tag="PKN90_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ85036.1"
                     /translation="MKSNHAEAIIEGLNSSQKEAVMYNNGPSLIIAGAGSGKTRVLTC
                     KIAYLLELGLSPERIVALTFTNKAAREMKERIASMVGQKIAKRLVMGTFHAVFSRILR
                     TESEHLTIKKDFVIYDTTDAKNTIKTIVKEMQLDEKMYKPALVLSRISYAKNNLLSPA
                     AYATSKLIDNDFYAKIPRVKDIYPVYVSRLKQSSALDFDDLLYYTNVLFRDFPKVKEK
                     YQDIFEFILVDEYQDINFAQHLIVKFLAEKHHRVCVVGDDAQSIYSFRGANIENILTF
                     KDTYPETRVFKLEQNYRSTQNIVGAANSLISKNKFKIKKDVFSENEPGEKIHVLTAYS
                     DIEEGYIVANKMQELYHQTTPNSWNDFVVLYRTNSQSRLIEDALRKQSIPYRIYGGVS
                     FYQRAEVKDVLAYIRLAINNDDEEAFKRIVNSPSRGIGNTTLAKVFAKAQLFQVSAFQ
                     VSANPMQYNVEINAGTATKLINFTNLIHDFTLQMSQMDAYQVASYITRNAGIIRNLQE
                     SKTAENISALENIDELLSSIYEYAEQKKEEGEEHPSIAEYLSEISLLTDQDTGRDAEE
                     NRVTLMTIHLAKGLEFKNVFIVGVEEDLLPSVYADDEKGLEEERRLFYVAITRAEKRC
                     FISYSKSRFRNGQMTYAKHSRFISDIDSHFLHLPVDFSSKNTSHFDFSQQKESFFSSN
                     KPLTQTVIPKPTYNKNLTKLSAISNKTSSQPVLSEVTIGSRIEHERFGKGVIIAITGD
                     AADTRITVKFDNAGERVLLLKFAKFSLC"
     gene            2448..>4217
                     /locus_tag="PKN90_01765"
     CDS             2448..>4217
                     /locus_tag="PKN90_01765"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ85037.1"
                     /translation="MLISSRHIIFLLIICTNILHVFANENMVVRGFVLNEQQQPIEMV
                     DVSVQQSPTRTVTDKFGKFEIHVSAQDSFFLVFSSIAHRPAIQKVYVNEKYVTNVTVV
                     LQDVINELTDIEVKGEKHQHTTAEWVNTEFIKVLPSASGKSIESLIKSFSGVSSTNEL
                     SSQYSVRGGSYDENSVYVNGIEIYRPFLIRSGEQEGLSFINTDMVGEVTFSAGGFDAR
                     YGDKLSSVLDITYKQPSTFEGNVAVSFAGASAYIGSATNKWTQMHGLRYKNASYLLGT
                     LQTKAEYNPNFIDYQSFVTYKPSTNWSFSLLGNVSQNVYNFVPESRETTFGTLENLQK
                     FTIYFDGQEKDIFRTGFGAFSTTYQPNKKVGLTFIASAFATNEQENYDIKGEYWLNKV
                     SSASSSSEEVMGVGTYFEHARNEMNVFVCKATHHGTFDINRSHAIVWGVDAVSEKVID
                     AVNEWEMRDSAGYSLPYSPTEIRLYESLSSAHTFYSTRLRSYLQYSYKNISHHGLFVL
                     TSGLRCNYWDWNNEMTISPRITASYFPDWQQRINFRFATGVYYQTPSFKEIKDTITKN
                     GNLTHFLNKNIQSQRSLQFIAGTD"
BASE COUNT         1430 a          645 c          787 g         1355 t
ORIGIN      
        1 gctaatgccc agccatatta tgtggtttta aatgcagcag gcaagccagt agctccttct
       61 tatgctttta acaaggatgt aaaagctttt gaaacatatt taaagcaagc gattaagacc
      121 tataaaaatg aaaagtaacc atgcagaggc tattattgaa ggattaaatt cttctcaaaa
      181 agaagctgtt atgtataata atggtccttc gttgattatt gctggagcag ggtctggaaa
      241 aacacgtgtg ttaacgtgta aaattgctta tttattagaa ttaggtttgt ctcccgaacg
      301 aatagtagct cttactttta cgaataaagc tgccagagaa atgaaggaac gcattgcctc
      361 tatggtaggt cagaaaatag ctaaacgttt ggtaatgggg acttttcatg ctgttttttc
      421 acgtatatta cgcacagaat cagaacattt aactataaaa aaagattttg taatatacga
      481 tacaactgat gctaaaaata ctatcaaaac catagtaaaa gaaatgcaac ttgatgaaaa
      541 gatgtataag ccagcattgg tgttgtcaag aatttcttat gcaaaaaata acttgctatc
      601 accagcagcc tatgctacaa gtaaattaat tgataatgat ttttatgcaa aaattccacg
      661 agtaaaagat atttacccag tatatgtttc ccgtttaaaa cagtcatcag cattggattt
      721 tgatgattta ttgtactata caaatgtatt atttagagat ttccccaagg taaaagaaaa
      781 atatcaagat atttttgagt ttattttagt agacgagtac caagatatta attttgcaca
      841 acatttaatt gtgaagtttc tagccgaaaa acatcatcgt gtgtgcgtag ttggagacga
      901 tgcacaaagc atttattcgt ttagaggtgc caatattgaa aatatactta cgtttaaaga
      961 tacatatcct gaaacaagag tatttaaact tgagcaaaat tatcgctcta cacaaaatat
     1021 tgtaggagct gcaaatagtt tgatttcgaa aaataaattt aaaataaaaa aagacgtttt
     1081 ttccgagaat gaaccaggag aaaaaataca cgtccttacg gcttattcag atattgagga
     1141 gggatatatt gttgctaata aaatgcaaga attataccat caaacaactc ccaattcgtg
     1201 gaatgatttt gtagttttgt atcgtacaaa ctcacaatcg cgtttgattg aagatgcttt
     1261 gagaaaacaa tctataccgt atcgtattta tggaggagtg tcgttttacc aacgagcaga
     1321 agtaaaagac gtgttggcat acatacgttt agcaattaat aatgacgatg aagaggcgtt
     1381 taaacgaatt gtaaattctc cttcacgtgg gattggtaac acaactttgg caaaagtatt
     1441 tgccaaagcc cagctttttc aagtttcggc atttcaggtt tcggctaatc ctatgcaata
     1501 taatgtggaa ataaatgctg gaactgctac aaagttaatt aactttacca atttaataca
     1561 cgactttacc ttgcaaatga gtcagatgga tgcatatcaa gtagcatcat atattactcg
     1621 aaatgctgga attattcgca atttgcaaga atcaaaaaca gcagaaaata ttagtgcatt
     1681 agaaaatatt gatgaactat tgtctagtat atatgaatat gctgaacaaa aaaaagaaga
     1741 aggcgaagaa catcccagta ttgcagagta tttatcggaa atatccttgt tgacagacca
     1801 agatacgggt agagatgctg aagaaaatag ggtaacatta atgacaatac atttggctaa
     1861 agggttggaa tttaagaatg tgtttattgt aggtgttgaa gaagatttac tcccgtctgt
     1921 gtatgctgac gatgaaaaag gattggaaga agagaggcgt ttattttacg ttgctattac
     1981 tagagccgaa aaaagatgtt ttatctctta ttcaaaaagt cgctttagaa atggtcaaat
     2041 gacttatgct aaacatagtc gatttatttc tgatatagat tctcattttt tgcatttacc
     2101 cgttgacttt tcttctaaga atacaagtca ttttgatttt tcgcaacaaa aagagtcttt
     2161 ctttagttct aataagccat tgacacaaac tgtaatacca aaacctacat acaataaaaa
     2221 cttaactaag ttgtctgcta tcagcaataa aacttcatca caacctgttt tatctgaggt
     2281 tacaataggc tcacgtatcg aacatgaacg atttggaaaa ggtgttataa tagccattac
     2341 tggagatgct gcagatacac gtataactgt gaagtttgat aatgctggag aaagggtttt
     2401 attacttaaa tttgcgaaat tttcattatg ctaattcttt attatttatg ctaatttcgt
     2461 ctagacatat catctttttg cttatcattt gtacaaacat actacatgta tttgcaaatg
     2521 aaaatatggt agtgagagga tttgtactga atgaacagca acagcctatt gaaatggttg
     2581 atgtttctgt tcagcaatcg cctacaagaa ccgttacaga taaatttgga aaatttgaga
     2641 ttcatgtttc agcccaagat tctttctttt tagttttttc gtccattgca catcgccccg
     2701 ctatacaaaa ggtatatgta aatgaaaaat acgttaccaa tgtaactgta gtgttgcaag
     2761 atgtaataaa tgaattaacg gatattgaag taaaaggaga aaagcatcag catactacag
     2821 cagaatgggt aaatactgaa tttatcaaag tcttgccatc tgcctcaggc aaaagcattg
     2881 aaagtttgat taaatcattt tctggagtga gttcaaccaa tgagctgagt tctcagtatt
     2941 cagttagagg tggtagttat gatgaaaata gtgtttatgt taatggtata gagatttacc
     3001 gtccttttct tatccgttca ggagagcaag aagggttaag ttttatcaac acggatatgg
     3061 ttggggaggt aactttttca gctggtggat ttgacgctag atatggagat aaattatcct
     3121 ctgtgcttga tattacatat aaacaacctt ccacttttga aggaaatgtt gctgtcagct
     3181 ttgctggggc atctgcttat attggttcag caactaataa atggactcaa atgcatggct
     3241 tacgctataa aaatgcttct tatttgcttg gaacgttgca aactaaagca gagtataatc
     3301 ctaattttat tgattatcag tcttttgtta catataaacc cagtaccaat tggtctttct
     3361 ctctattagg caatgtatcc caaaatgtgt ataattttgt acccgaatca agagaaacaa
     3421 cttttgggac cttagaaaac ttgcaaaaat ttaccatcta ttttgatgga caagaaaaag
     3481 atatttttcg tacaggtttt ggagcgtttt caacaaccta tcagcccaat aaaaaagtcg
     3541 ggttaacttt tattgcttca gcatttgcaa ccaacgaaca agaaaattat gatattaaag
     3601 gcgagtattg gcttaataaa gtttcgtctg cgagtagtag ttcggaggag gtaatggggg
     3661 taggtacata ctttgaacat gcacgtaatg aaatgaatgt gtttgtttgt aaggctactc
     3721 atcatggaac ttttgatata aatcgttccc atgccattgt gtggggggta gatgctgtat
     3781 ccgaaaaagt aatagatgct gttaatgagt gggaaatgag ggattctgcg gggtattcat
     3841 tgccatatag ccccactgaa ataaggctat atgaaagctt atcttctgca cacacttttt
     3901 attccaccag attacgttct tatttgcagt atagttataa aaatatttct catcatggtt
     3961 tatttgtgct aacctcaggt ttgcgatgta attattggga ttggaataat gaaatgacca
     4021 ttagccctcg tatcaccgca tcatatttcc ctgattggca acaacggata aattttagat
     4081 ttgccacagg agtgtattat caaacgcctt cttttaaaga aattaaagat acaattacga
     4141 aaaatggtaa tttaactcat tttttaaata aaaatattca atctcaacgt tcgttacaat
     4201 ttattgcagg taccgat
//
LOCUS       DAOPUR010000066         4210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21254_length_4210_cov_3.634416, whole genome shotgun sequence.
ACCESSION   DAOPUR010000066 DAOPUR010000000
VERSION     DAOPUR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21254_length_4210_cov_3.634416"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKN90_01770"
     CDS             complement(<1..245)
                     /locus_tag="PKN90_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ85038.1"
                     /translation="MNVLFEDNHIIAINKSCSDIVQGDKTGDTPLVEKVKIYLKEKYN
                     KPGNVFCGVTHRLDRPVSGIVLFAKTSKALTRLNEMLR"
     gene            complement(250..987)
                     /gene="rlmB"
                     /locus_tag="PKN90_01775"
     CDS             complement(250..987)
                     /gene="rlmB"
                     /locus_tag="PKN90_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNZ85039.1"
                     /translation="MKKESEMIYGMHAIIEAIDAGKEIDKIIMKNDMQGRLSHDLFKA
                     IEGRNIPIMRVPVERLDRITKKNHQGVIAFTIPVAYQHIEDIIPTLYEDGKAPFIIVL
                     DGITDVRNFGAIARTCECAGVHAIVIPEKNSASINADAIKTSAGALLKIPVCREKNLT
                     NAIKFLKNSGLKIVATSDKADDNYTDISYTDALAIVMGAEDIGISIENQRLCDTIVKI
                     PIVGTISSLNVSVATGIMAYEVLKQRL"
     gene            complement(1025..2044)
                     /gene="recA"
                     /locus_tag="PKN90_01780"
     CDS             complement(1025..2044)
                     /gene="recA"
                     /locus_tag="PKN90_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNZ85040.1"
                     /translation="MATKDTENQAKVNVEKLKALQLALDKIEKDHGKGTIMKMGDKTV
                     VEVNTISTGSIGLNVALGVGGFPRGRVIEIFGPESSGKTTLAIHAIAQAQKAGGIAAI
                     IDAEHAFDRFYAEKLGVDINNLLISQPDNGEQALEIADQLIRSSAIDIVVIDSVAALT
                     PKAEIEGDMGDSKMGLQARLMSQALRKLTANINRTNTTCIFINQLRDKIGITFGNPET
                     TTGGNALKFYASIRLDIRRIGQIKDGDEVIGNQTRVKIVKNKVAPPFRKAEFDIMFGE
                     GISIAGEIIDLGVEQGIIKKSGSWFSYGDTKLGQGRDAAKKVMIDNPELAAELEQKIM
                     EALAK"
     gene            complement(2198..3244)
                     /gene="ilvC"
                     /locus_tag="PKN90_01785"
     CDS             complement(2198..3244)
                     /gene="ilvC"
                     /locus_tag="PKN90_01785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNZ85041.1"
                     /translation="MAQMNFGGVVENVVTREEFPLEKAREVLKNETIAIIGYGVQGPG
                     QSLNLRDNGFNVIIGQRKGGKTWEKAIADGWVPGETLFDIEEACQKATIIQYLLSDAA
                     QIEVWPRIKPYLTAGKALYFSHGFGITYKERTGIVPPADVDVILVAPKGSGTSLRRMF
                     LQGRGLNSSYAIFQDATGKAFERVVALGIGVGSGYLFETTFKKEVYSDLTGERGTLMG
                     AIQGILLAQYEVLRENGHSPSEAFNETVEELTQSLMPLFAENGMDWMYANCSTTAQRG
                     ALDWMTPFHDATKPVFKKLYEEVACGNEAQRSIDTNSKPDYREGLEKELKALRESEMW
                     QAGAVVRKLRPENK"
     gene            complement(3411..3968)
                     /gene="ilvN"
                     /locus_tag="PKN90_01790"
     CDS             complement(3411..3968)
                     /gene="ilvN"
                     /locus_tag="PKN90_01790"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNZ85042.1"
                     /translation="MEKKLYTITVFSENMPGILNQITIVFTRRQLNIETLSVSPSALK
                     GIHKFTITVETTLDVVRKVVTQIDKRIDVIKAYYNTDDELVVQEIALYKVATDKVLEL
                     GGIEQLIRKHNIRILELTPTCIVFENTGMYEETQHLFEELQNSIGVLQFIRSGRVAIT
                     KSKVERLSDMLASIEECIKQQKIVK"
     gene            complement(3972..>4210)
                     /locus_tag="PKN90_01795"
     CDS             complement(3972..>4210)
                     /locus_tag="PKN90_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ85043.1"
                     /translation="HQERYSSTPMENPDFITIAKAYNINGKEVSERHELDMAIKEMLA
                     DDKPYLLVVNVEKNNMVYPMIPAGAAVTNILLGE"
BASE COUNT         1196 a          806 c          716 g         1492 t
ORIGIN      
        1 cgtaacatct cgtttaatcg ggtaagagcc ttgctcgttt tagcaaatag cacaatacca
       61 ctcacaggac gatcgagtcg atgagtaaca ccgcaaaata cattaccagg tttattgtat
      121 ttttctttga ggtaaatttt caccttttca accaaaggag tatcacctgt tttatcgcct
      181 tgtacaatat ctgaacatga tttgttgata gcaattatat ggttatcttc gaataataca
      241 ttcatcttct tacaagcgtt gtttcagtac ttcatacgcc ataataccag tggctacaga
      301 tacatttaaa gacgaaatag tgcccactat cgggatttta actatagtat cgcacaaacg
      361 ttggttttcg atagaaatac ctatatcttc agcccccatt acaatagcaa gtgcatctgt
      421 gtatgatata tccgtataat tatcatcagc tttatctgat gtagccacaa tctttagacc
      481 agagttttta aggaatttta tggcattggt caagtttttt tctcgacaaa caggtatttt
      541 gagtaatgct ccagccgaag tttttatggc atcggcattg atagaagcac tatttttttc
      601 gggaataaca atggcatgta cacctgcaca ttcgcaggta cgtgctatgg ctccaaaatt
      661 acgtacatcg gttataccgt caagtactat aataaatggt gcttttccat cttcatagag
      721 ggtaggaata atatcttcga tatgttgata ggcaaccggt attgtaaaag caattactcc
      781 ttgatggttt ttcttggtaa ttctgtctaa gcgttcgaca ggaacacgca taatgggtat
      841 atttctaccc tcaattgcct taaacaagtc gtgagacaag cgtccttgca tatcattttt
      901 catgataatt ttatcaattt ccttgccggc atcaatggct tcgataatgg catgcatgcc
      961 atatatcatt tccgattctt ttttcatgtg tgttctttgc gtaaatgtgt gttgatatgg
     1021 aactttattt tgctaaagct tccattattt tttgttccaa ttcggcagct aattctggat
     1081 tgtctatcat tactttttta gcagcatctc taccttgtcc tagtttggtg tcgccgtagc
     1141 taaaccacga tccgcttttt ttaataatgc cttgttcaac tcctaaatca ataatttctc
     1201 ctgcaataga aattccttca ccaaacatga tatcaaattc agctttacgg aaaggaggag
     1261 ctaccttatt ttttacaatt tttacgcgtg tttgattgcc aatcacttcg tctccatctt
     1321 taatttgtcc tatacgacga atgtctaaac gaatagaagc gtagaatttc agtgcatttc
     1381 cccccgtagt tgtttcagga ttgccaaatg taatgccaat tttatcgcgt aattggttaa
     1441 taaatataca cgttgtattt gttctgttaa tattggctgt aagtttacgt aatgcttgcg
     1501 acattagtcg tgcttgtaat cccatttttg aatcacccat atcgccttca atttcagctt
     1561 ttggtgttaa ggcggcaaca gagtctataa ctactatatc tattgctgag gagcgaatga
     1621 gttggtcggc aatttcgaga gcttgttctc cgttatctgg ttgagaaata agtagattat
     1681 tgatatcaac ccctaatttt tcggcataga aacgatcgaa tgcatgttca gcatcaataa
     1741 tagcagcgat tccaccagct ttttgtgcct gagctatagc atgaatggct agggttgttt
     1801 taccagacga ttcaggacca aaaatttcta ttactcttcc acgaggaaaa ccaccaacgc
     1861 ctaaagcaac gtttagtcca atagaacctg tagaaatagt gtttacttct actaccgttt
     1921 tatcgcccat tttcataatg gtgccttttc cgtggtcttt ttcaatctta tcaagtgcta
     1981 attgaagtgc ttttaatttt tctacattca cttttgcttg attttcagtg tcttttgtcg
     2041 ccatagttgt atcagtttat ttgaacatca aaagtataat tttttttgtc aatagccaat
     2101 tcattttgtt agtttgttga aaagtttatg aacaaaaaaa gcggacaaat atcttttgga
     2161 tagtgtccac ttttaaaaag tgcaattaaa tgcgttatta tttattttct ggacgcaatt
     2221 tacgtactac agcacctgct tgccacattt cgctttcacg aagtgcttta agttcttttt
     2281 ctaaaccttc gcggtaatca ggtttagagt tggtgtcgat agagcgttga gcttcgttac
     2341 cacaagcaac ttcttcgtat aattttttga atacaggttt agtagcatcg tggaaaggag
     2401 tcatccaatc taatgcacca cgttgagctg tagttgagca gttagcatac atccagtcca
     2461 ttccgttttc tgcaaacaat ggcattaatg attgagttaa ttcttctact gtttcgttaa
     2521 acgcttcaga aggagaatgt ccgttttcgc gtaacacttc atattgagct aacaaaatac
     2581 cttggatagc acccattaat gtaccacgtt cgccagttaa atcagaatat acttcttttt
     2641 tgaaagtagt ttcaaacaag taaccagaac ctacaccaat acctaaagca acaacacgtt
     2701 caaaagcttt gcctgtagca tcttggaata ttgcgtaaga tgaatttaat ccacgtcctt
     2761 gaaggaacat acgacgaagt gatgtaccag aacctttagg tgcaactaag ataacatcta
     2821 catcagctgg aggcacaatg ccagtacgtt ctttgtaagt aataccaaaa ccatgagaga
     2881 aatacaaggc ttttcctgca gttaggtatg gtttgatgcg aggccacact tcaatttgag
     2941 ctgcatctga caataaatat tgaataatgg ttgctttttg acaagcttct tcaatgtcaa
     3001 acaatgtttc gccaggaacc cagccgtcag caattgcttt ttcccatgtt tttccacctt
     3061 tacgttgtcc aatgattaca ttgaatccat tatcacgtaa gttaagcgat tgaccaggac
     3121 cttgaacacc ataaccgata attgcaatgg tttcgttttt tagcacttca cgtgcttttt
     3181 ctaaaggaaa ctcttcgcgg gtaactacgt tttctactac gccgccaaaa ttcatttgtg
     3241 ccattttact attattttat ttattaaaag gtaattagtt ctttttaatt tggtttaaac
     3301 atctctacaa attattttat agaaaagcac gacaaaatta cttttttttt attgttttgg
     3361 caaatattta tgctaatttg tcgtgcaaag actatctttt actttcgaat ttactttact
     3421 attttttgtt gtttaatgca ttcttcgata gaagccaaca tatcactaag tcgttcgacc
     3481 ttactttttg taatagccac tctaccagaa cgaataaatt gtaatactcc gatagaattt
     3541 tgtagctctt caaacaaatg ttgtgtttct tcgtacatcc ctgtattttc aaacacaata
     3601 caggttgggg tgagttctaa gatgcgaata ttgtgcttac gaataagctg ttcgatacct
     3661 cctaattcca acaccttgtc agtggctact ttgtataatg caatttcttg tacaactaac
     3721 tcatcatcag tgttgtaata agctttaatt acatcaatac gtttatcaat ttgagttact
     3781 acttttcgaa caacatctaa ggtagtttca acagtaatgg taaatttgtg tattcctttg
     3841 agagcagaag gagaaacaga aagagtttca atattcagct gtctacgagt aaatacaatg
     3901 gtaatttgat ttaaaatacc cggcatattt tctgaaaata ccgtgattgt ataaagtttt
     3961 ttttccataa gttattctcc taataaaatg tttgtaacgg ctgctcctgc aggaatcatt
     4021 gggtaaacca tgttgttttt ttctacattt actaccaaca aatacggttt atcgtctgct
     4081 aacatttctt tgattgccat atctaattca tgtcgttctg acacttcttt tccattaata
     4141 ttgtatgctt tggctatcgt tataaagtct ggattttcca ttggtgttga tgaatagcgt
     4201 tcttggtgaa
//
LOCUS       DAOPUR010000067         4199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21333_length_4199_cov_3.382239, whole genome shotgun sequence.
ACCESSION   DAOPUR010000067 DAOPUR010000000
VERSION     DAOPUR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21333_length_4199_cov_3.382239"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKN90_01800"
     CDS             complement(<1..212)
                     /locus_tag="PKN90_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNZ85044.1"
                     /translation="MQNTTNTFDYHVDKFADIEILRYRVTGFEALSLQQKQLIYHLSE
                     AAQEGRDILFDQHYEHNLCIRRTLEA"
     gene            complement(220..1041)
                     /locus_tag="PKN90_01805"
     CDS             complement(220..1041)
                     /locus_tag="PKN90_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85045.1"
                     /translation="MKKIALLILFTLLCVSHVVYAQTIQENEQMIVSWFEDIKETQSD
                     SAKIQLNKKIEQLLYTTLQEPNAFNYSFPALKYIGSIYAPDNLMRVFSWNYPLSNGKY
                     GYSALFMFKNKQKSTPSLHLVSTTTAFKPEETRKYDPSTWYGALYYNVIAHKISRDKT
                     EYLLLGISNYQPITKVKIIESIKISNRQLTFGTPLFKKDKKTLYRAVFEYKKEVNMLL
                     EYESSKKRFVFDHLSPSDPIYKDVYHFYGPDFSYDSYQWKKKKLHYTPDIDVRNK"
     gene            complement(1114..2943)
                     /locus_tag="PKN90_01810"
     CDS             complement(1114..2943)
                     /locus_tag="PKN90_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNZ85046.1"
                     /translation="MYNDIPLSCFLPTTQKEIKALGWEELDVILFSGDAYIDHPSFGI
                     AVMGRILEQQGLKVAIVPQPNWRDDLRDFKKLGKPRLFFGVSAGSMDSMVNHYTANKR
                     LRSNDAYTPEGRAGYRPDYATVTYCTILKSLYPDTPIVIGGVEASLRRVTHYDYWSDK
                     LLPSILVDSQADLLVYGMGEKAIVELVQLLQQNIPFQQLHDIPQTAFLIDKTANSQST
                     GEWESLSLFSHEECIASKINQAKNFRVIEEHSNSQKAGRLLQDVGTKTVIINPPYPTM
                     TEKEIDQTYTLPYTRLPHPRYKGKTIPAYEMIKHSVTLHRGCFGGCAFCTISAHQGKF
                     IASRSEKSILAEIEKITQMPDFKGYISDIGGPSANMYKMQGIDLTICEKCKKPSCIHP
                     KICKNLDTNHAPLIELYKKVRSIKEIKKAFVGSGIRYDMILHKTGNKQTDASNMEYAN
                     ELIAHHVSGRLKVAPEHTNDEVLNLMRKPSFEYFETFVKLFNEVNKQHHLNQQLIPYF
                     ISSHPGCKQQHMAELAAKTKALDFKLEQVQDFTPTPMTLATEMYYTGIHPYTLEKISA
                     PNTPQQKLNQRKFFFWYQKEYKNEIINDLKKMNRMDLIKKLYP"
     gene            complement(2966..>4199)
                     /locus_tag="PKN90_01815"
     CDS             complement(2966..>4199)
                     /locus_tag="PKN90_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ85047.1"
                     /translation="TKGLSVSEVSSMLKGTPSTTVQIVVERMGMKKPIHYSIVREKIY
                     LNPVTYAGILPGKSNIAYIRLNSFTENCAEEVKKALQDLAKKGATSLILDLRGNGGGV
                     INDAIAISGLFLPKGTEIVSTKGKNAEMDRSYKTSSDPLFPNLPLAILVNESSASAAE
                     IVAGAMQDLDRGIIIGRNTYGKGVIQSTYPLPYNTNIKITIAKYYTPSGRCIQSSKHI
                     ARLASTNSDSIEHVFATQNGRVVKEGQGIMPDILVPASTLKTITYHLVSNAYFYDFVN
                     IYTQQHATIPPIEEFAVSDDLFNQYKDYIIKQNFTYELQSEKGLQKLIETAKNEGYYD
                     ENKVYFSDLEKVLKHDINQDIIDAKEEISELLAAEIVKRYYFQRGELQQLLKNDKELK
                     RAIEIVSDQDTYQTMLKP"
BASE COUNT         1173 a          718 c          750 g         1558 t
ORIGIN      
        1 atggcttcga gtgtgcgtcg aatacacaaa ttgtgttcat aatgttgatc gaataagata
       61 tctcgccctt cttgtgccgc ttctgaaaga tgataaatta attgtttttg ttgcaatgac
      121 aaggcttcaa agccagtaac acgatatctc aatatttcaa tatcggcaaa tttatctacg
      181 tgataatcaa atgtatttgt tgtattttgc atagttgttc tatttatttc ttacatcaat
      241 atctggtgtg tagtgaagtt ttttcttttt ccattgatag gagtcatacg aaaaatcagg
      301 accataaaaa tgatacacat ctttgtatat agggtcagat ggtgaaagat ggtcgaatac
      361 aaatcgtttt ttgcttgact catattctaa taacatgttt acctcctttt tatattcaaa
      421 aacggctcga tagagtgtct ttttatcttt tttaaaaagg ggagtgccaa atgttagttg
      481 ccgattacta atttttatag attcaattat ttttactttt gtaatgggtt ggtaattgct
      541 gatgcccagt aataggtatt ctgttttatc tcggctaatt ttgtgtgcta ttacgttgta
      601 atacaatgct ccataccacg ttgaagggtc gtatttgcgt gtttcttcag gtttaaaagc
      661 tgtagttgtc gatacaaggt gcaacgaggg tgtgcttttt tgcttgtttt tgaacataaa
      721 tagtgccgaa tagccatatt ttccattgct taatggataa ttccaagaaa aaacacgcat
      781 tagattgtca ggagcatata tacttccaat gtattttaag gcaggaaatg aataattaaa
      841 cgcatttggc tcttgcaatg ttgtgtaaag caattgttca atctttttat tgagttgtat
      901 ctttgcacta tcagattgag tctcttttat atcttcaaac cacgaaacaa tcatttgttc
      961 gttttcttgt attgtttgtg catatactac atggcttaca cataacaagg tgaaaagaat
     1021 aagtagtgcg atttttttca ttggatttct attagtttac gctactgcta cacagtttta
     1081 aatattggta atattctgat ttactggact atactatgga tatagttttt taattaagtc
     1141 cattctgttc atctttttta aatcgttgat aatctcattt ttatattctt tttgatacca
     1201 aaagaaaaac tttcgttgat tgagtttttg ctgaggtgtg tttggtgctg aaattttctc
     1261 caaggtatat ggatgtatac ccgtataata catttccgtt gctagtgtca tcggagttgg
     1321 tgtaaaatct tgtacctgct ctaatttgaa atccaatgct tttgttttgg cagctaattc
     1381 agccatgtgc tgttgtttac accctggatg actcgagata aagtagggta taagttgttg
     1441 atttaaatgg tgttgcttgt tcacttcgtt gaatagctta acgaatgttt caaaatactc
     1501 aaaagatggt tttcgcatca agttaaggac ttcatcgttg gtatgctcag gagctacttt
     1561 tagtcgacca gatacgtggt gtgcaattaa ttcgttcgcg tactccatat tggatgcgtc
     1621 tgtttgctta ttacccgttt tatgcaatat catatcatag cgaataccac taccaacaaa
     1681 tgcttttttt atttctttaa tgctgcgtac ttttttatac aattcaatca atggagcatg
     1741 attagtatcc aaatttttac aaatttttgg atgaatacac gatggtttct tgcatttttc
     1801 gcaaattgtt aaatcaatac cttgcatttt atacatattc gctgatggac ctccaatatc
     1861 gctaatatat cctttaaaat caggcatttg agttatcttt tcaatttctg ctaaaatgga
     1921 tttttctgaa cgtgatgcaa taaatttgcc ctgatgtgca gaaatagtac aaaaagcaca
     1981 accaccaaaa catcctctgt gtaacgtaac agagtgtttt atcatttcgt atgcaggaat
     2041 ggtttttcct ttgtaccgtg gatgtggcaa gcgtgtgtat ggcaaagtgt aggtttggtc
     2101 aatttctttc tccgtcatcg tcggataagg tggattaata atcactgttt ttgttcctac
     2161 atcttgcagt aatctaccag ctttctgcga attggaatgt tcttcgataa ccctaaaatt
     2221 ctttgcttgg tttatttttg atgctataca ttcctcgtga gaaaacaatg agagagattc
     2281 ccactctcca gttgactgag aattggctgt tttgtcaatt aaaaaagcag tttgagggat
     2341 atcgtgtagt tgttgaaaag ggatgttttg ttgcaaaagt tgcaccaatt ctacaatcgc
     2401 tttttcgccc attccatata caagtaaatc tgcttggcta tcaactaaga tgcttggcaa
     2461 taatttatca gaccaataat catagtgtgt aacacgtctt aatgaagctt ctacgcctcc
     2521 aattacaata ggagtatcgg gatacaatga tttcagtatt gtgcaatagg taacagtcgc
     2581 gtaatcagga cgataaccag ctcttccttc aggcgtgtaa gcatcgttgg agcgtaagcg
     2641 tttgttggct gtataatgat ttaccatcga atccatacta ccagccgaaa caccaaagaa
     2701 caaacgaggt tttcctaatt tcttaaaatc acgtaaatca tctcgccagt tgggttgtgg
     2761 cacaatagca acttttaagc cttgttgttc aagaattctt cccattacag ctattccaaa
     2821 ggagggatgg tcgatatacg catctcctga gaacagaata acgtccaatt cttcccaacc
     2881 taatgctttt atttcttttt gggtcgtagg taaaaaacag gataatggta tgtcgttata
     2941 cataattcaa cacagattta tataattaag gctttaacat cgtttggtat gtatcttggt
     3001 cgcttacaat ctctatggca cgttttagct ctttgtcatt ttttaataac tgttgcaatt
     3061 cgcctcgttg aaaataatag cgttttacaa tttcagcagc caataattcg ctaatttctt
     3121 ctttagcatc aataatatct tgattgatat catgtttcaa tactttttct aagtctgaaa
     3181 aatacacttt attttcgtcg taatatcctt catttttcgc tgtttctatc aatttttgta
     3241 atcctttttc tgattgcagt tcatatgtaa aattctgttt tataatataa tctttgtatt
     3301 gattaaataa atcatctgat accgcaaatt cttcaatcgg aggaatcgtt gcatgttgtt
     3361 gtgtatatat atttacaaaa tcgtagaagt aagcattaga tactaaatga taggtaatcg
     3421 ttttgagtgt cgatgcagga acgagtatgt ctggcataat gccttgacct tctttcacta
     3481 ctctaccatt ttgtgtagca aagacatgct ctatgctatc agagttagtg cttgctaaac
     3541 gtgcaatatg tttactactc tgaatacaac gtccacttgg tgtataatat ttggctattg
     3601 ttattttaat atttgtgtta taaggtaaag ggtaggtgga ttgaattact cctttaccat
     3661 aggtatttcg accaatgatg atgcctctat ctaggtcttg catagctcct gctacaattt
     3721 cagctgcaga agcagagctt tcatttacta atatggctaa agggaggtta ggaaataaag
     3781 gatcgctact ggttttatac gatctatcca tttccgcgtt ttttcccttt gttgagacta
     3841 tttcagttcc ttttggtaga aataagccac ttatggcaat ggcgtcgtta attactccac
     3901 ctccattacc acgtaaatct aaaattagtg atgtagctcc tttcttagct aagtcttgta
     3961 aggctttttt tacttcttct gcacaatttt cagtaaaact gttcaaacga atgtacgcta
     4021 tgttgctttt acctggtaat attccagcat aagtaacggg gtttaggtat attttctctc
     4081 ttacaatgga ataatgtatg ggttttttca ttcccatacg ttcaacaaca atctgtacgg
     4141 tcgttgatgg agttcctttt aacatactac ttacttccga aacagacaat cctttggta
//
LOCUS       DAOPUR010000068         4195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21364_length_4195_cov_3.946135, whole genome shotgun sequence.
ACCESSION   DAOPUR010000068 DAOPUR010000000
VERSION     DAOPUR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21364_length_4195_cov_3.946135"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKN90_01820"
     CDS             complement(<1..789)
                     /locus_tag="PKN90_01820"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNZ85048.1"
                     /translation="MKQYHELLQRVLDEGTQKEDRTGTGTISVFGHQMRFKMEDGFPC
                     LTTKKLHLKSIIHELLWFLNGDTNVRYLQENGVRIWNEWADEKGDLGHIYGYQWRSWP
                     TYDGGFIDQISEAVHTIKNSPDSRRIIVSAWNVADLPNMNLPPCHAFFQFYVANGKLS
                     LQLYQRSADIFLGVPFNIASYALLLQMMAQVTGLQAGDFIHTLGDAHIYTNHLEQVQL
                     QLTREPRQLPTMKINPTITSIFDFQFSDFELVNYDPYPHIPGAVA"
     gene            complement(824..2797)
                     /locus_tag="PKN90_01825"
     CDS             complement(824..2797)
                     /locus_tag="PKN90_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ85049.1"
                     /translation="MQFKFSILFLLILVTLSSCSIQARINKADKKYELGEYYVAGNLY
                     KRIYPSIPSKEKELRASVAFKSGNAFRQINDNKRAETAYRNAIRYKYKEPLVYFYAAE
                     VARKNNKHTDAIKFYETFLESNPSHREALNGLSSSKLVLSDWKKPTRYVVSKEELFNS
                     RFSDFSPSFANSRGDLVFFNSTRKLNKKNKASRITGQNNNNIFSSRKNIQGKWEEPLL
                     VEGDINSEFDEGTCSFSFDGQELFFTRSKIEKGKTLGTGIFVSKRSGGSWTSPQQIIL
                     LQDSSLNIAHPALSPNGDCLFFVSDMSGGHGGKDIWKVERTKDGWGIPKNLGETINTA
                     GDEMFPSFKKNGTLYFSSDGHPGFGGLDIFKAEPSENNEWMISNMMSPVNSEADDFGI
                     TFMEKEEKGFFSSNRADRRFFDNIWSFELPKLEYKIEGKVTDNNGDILGDAVIRLVGS
                     NGTIEKISVRRNGTFSQIIAPNANYILLASARGFLNSSQKVSTENSNKNQTFTLNFQL
                     NSISRPVQMNNIFFKFGSAELTPESAVALNELVNLLKDNPTVTIEIAAHTDMIGTETA
                     NTLLSTQRAQSVVDYVKQKGIDTARLIPKGYGKNKPVEVDKTLAKKYTFLSEGVILDE
                     EFITSLPSQQQEIANQINRRTEFKVLKTTFQAY"
     gene            complement(2914..3618)
                     /locus_tag="PKN90_01830"
     CDS             complement(2914..3618)
                     /locus_tag="PKN90_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ85050.1"
                     /translation="MNEQKVKILLCEDDENLGMLLREYLQAKGYDTDLLPDGDAGYKA
                     FMKNTYDVCVFDVMMPKKDGFTLAQEVRAVNAEVPILFLTAKTLKEDILEGFKIGGDD
                     YITKPFSMEELLFRIEAILRRIKGKKLKEKIVFKIGKFVFDSQKQILSIGDKHTKLTT
                     KESELLGLLCANANDILERNYTLKTIWIDDNYFNARSMDVYITKLRKLLKEDESVQII
                     NIHGKGYKLIVPQGVE"
     gene            complement(3611..>4195)
                     /locus_tag="PKN90_01835"
     CDS             complement(3611..>4195)
                     /locus_tag="PKN90_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ85051.1"
                     /translation="SISNIIYDETQRLSFQVEKVLETSILEKEKSIMKFKEANVHEII
                     ENVCNNFSIKISSKGGIINTNLQASEYWAMVDEMHFTNVIYNLLDNALKYRNLDQSPI
                     LKIKSWNEKDILYISVEDNGIGIKKEHLKKIFEKFHRVPTGNVHNAKGFGLGLAYVKK
                     IIEKHKGSIRTESELHKGTKFIITIPSLKKTQYE"
BASE COUNT         1177 a          734 c          703 g         1581 t
ORIGIN      
        1 cgccacagct ccaggtatat gaggatacgg atcataattt actaattcaa aatcactaaa
       61 ttgaaaatca aagatagatg ttatggtagg gttgattttc atggttggta attgtcgagg
      121 ttcacgtgta agttgcagtt gaacctgttc cagatggttg gtgtaaatat gtgcatctcc
      181 caatgtgtga ataaaatcac ctgcttgtag cccagttact tgtgccatca tttgaagtaa
      241 tagggcgtat gatgctatat taaacggaac acctaaaaaa atatcggcac ttcgttggta
      301 caattgcaaa ctaagtttgc cattcgctac ataaaactga aaaaaagcgt ggcatggagg
      361 taagttcata ttgggcaaat cggccacatt ccacgcactt acaattattc ggcgtgaatc
      421 agggctgttt ttaatggtat gtacagcttc tgatatttgg tcgataaaac caccatcata
      481 cgtgggccac gaacgccatt ggtagccgta aatatgcccc aaatcaccct tttcgtcagc
      541 ccattcgttc catatacgaa cgccattttc ttgtaaataa cgtacatttg tatcgccatt
      601 taaaaaccac agtaattcgt gtatgatgga ttttagatgc agtttttttg tggttaaaca
      661 cggaaatccg tcttccattt taaaacgcat ttggtgtcca aatacactta ttgtacctgt
      721 accagttcgg tcttcttttt gtgtaccttc atctagtact cgttgaagta attcgtggta
      781 ttgtttcata ttactaattg ttcatatcca tttgtttcac tattcaataa gcctgaaagg
      841 ttgtttttag tactttaaac tctgtacgtc tgtttatttg attagcaatt tcttgttgct
      901 gagaaggtaa agatgtaata aattcctcat caagtataac tccttctgat aaaaaagtat
      961 attttttggc gagtgtttta tctacttcaa ctggtttatt ttttccgtat ccttttggga
     1021 taagacgagc agtgtctatt cctttttgtt ttacataatc tactactgat tgagctcgtt
     1081 gtgtggataa aagtgtgttt gctgtttctg taccaatcat gtccgtatgt gctgcaattt
     1141 caatagttac cgttggatta tcctttagta gattaaccaa ttcgtttaaa gctactgcag
     1201 attctggtgt gagttctgct gatccaaatt taaagaaaat attattcatt tgcactggtc
     1261 gactgattga gtttaattga aaattgagtg taaaggtttg gtttttattc gaattttcgg
     1321 tagaaacttt ttgcgagcta tttaaaaatc ctcgtgcaga tgccaataaa atatagttcg
     1381 catttggggc aattatttgt gaaaatgtcc cattgcgacg aacagatatt ttctcaatag
     1441 ttccattcga tcctactagt cttatcacag catcaccaag tatatctcca ttattatctg
     1501 ttacttttcc ttctattttg tattcaagtt ttggtagttc aaagctccat atattgtcaa
     1561 agaaacgtct gtcagcacgg ttgctcgaaa agaacccttt ttcttctttt tccatgaatg
     1621 taattccaaa atcatcggct tctgagttca ctgggctcat catattgctt atcatccatt
     1681 cattgttttc ggatggttcg gctttaaaaa tatctaatcc gccgaaacca ggatgcccat
     1741 cggaagaaaa atacaacgta ccattttttt taaacgaagg aaacatttca tctccagctg
     1801 tgttaattgt ttctccaaga ttttttggaa ttccccatcc atcttttgtt ctttccactt
     1861 tccaaatatc cttgcctcca tgtccgcctg acatatcaga aacaaagaat aaacagtctc
     1921 cattgggaga aagggctgga tgagcaatgt tcaaactcga gtcttgtaat aagatgattt
     1981 gttggggaga tgtccatgaa cctccacttc ttttagaaac gaaaataccc gtacctagtg
     2041 ttttcccttt ttcaatttta ctacgggtaa aaaatagctc ttgaccatca aatgaaaatg
     2101 aacaggtgcc ttcatcaaat tctgaattaa tatctccttc tactaggaga ggctcttccc
     2161 attttccttg aatattttta cgggaactaa aaatattatt attgttttgt cccgtaatgc
     2221 ggcttgcttt atttttttta tttaattttc tggtggaatt aaaaaaaact aagtctcctc
     2281 tgctgtttgc aaatgaaggg gaaaaatcag aaaaacgaga attgaaaagt tcttctttgc
     2341 taacaacata tcgagtgggt tttttccaat cagatagtac taatttggac gaagaaagtc
     2401 catttaatgc ttcacgatgt gaagggttcg attctaaaaa cgtttcgtaa aatttgatgg
     2461 catctgtatg tttgttattt ttgcgggcaa cttctgctgc ataaaaatag accaaaggtt
     2521 ctttgtattt gtaacgtatg gcgttacgat aggctgtttc tgctctttta ttatcgttaa
     2581 tttgacgaaa tgcattccca gatttaaatg cgacacttgc tcttaattct ttttctttag
     2641 aaggtataga tggataaatt cttttgtata aattacccgc tacgtaatac tctcctaatt
     2701 catatttttt gtctgctttg tttattcgag cctgtatgct acacgaactg agtgtaacaa
     2761 gtataagaag aaataaaata ctaaacttaa attgcatatg aattgtgaat ggcttatgag
     2821 agtacaaata tacaatttct tttttaaaaa atagatagaa aaaaacctca tcatatttta
     2881 atgatgaggt ttttttctat ttcaaggtaa agattattcc actccttgag gaacaattaa
     2941 tttataccct tttccatgaa tgttaattat ttgcacactt tcgtcttctt tcaataattt
     3001 acgaagtttt gtaatgtata cgtccatgct tcgagcattg aaatagttat catctatcca
     3061 aattgttttt aatgtataat ttctttctaa tatgtcatta gcattggcac ataataaccc
     3121 aagtagttct gattcttttg tggttaattt tgtatgttta tctcctattg acagaatttg
     3181 tttttgcgaa tcaaatacaa atttaccgat tttaaataca attttttcct ttaatttttt
     3241 tccttttata cgacgcagaa ttgcttctat tctgaatagt aattcttcca tgctaaaagg
     3301 ttttgtaatg taatcatctc ctccaatttt aaagccttcg aggatgtcct ctttgagggt
     3361 tttagctgtt aaaaacaaaa taggcacttc ggcatttact gctcgcactt cttgggctag
     3421 tgtaaatcca tcttttttgg gcatcattac gtcgaataca catacgtcgt aggtgttttt
     3481 cataaatgct ttgtatccag catctccatc tggaagtagg tctgtatcat agcctttggc
     3541 ttgtaggtat tcgcgtaaaa gcatacctaa attttcgtca tcttcgcata gaagaatctt
     3601 tactttttgt tcattcatat tgtgtttttt ttagtgatgg tattgttatg ataaattttg
     3661 ttcctttatg tagttcactt tctgttcgaa tagagccttt gtgtttttca ataatttttt
     3721 ttacataagc caaacccaag ccaaaccctt ttgcgttgtg tacatttccc gtaggaacac
     3781 gatgaaattt ttcaaatatt tttttcaaat gttccttttt gattcctatt ccattatctt
     3841 ctacactaat gtaaaggata tctttttcgt tccatgattt tattttaagg ataggacttt
     3901 ggtcaagatt tcgatatttt aatgcattgt ctaggaggtt gtaaattacg ttggtaaagt
     3961 gcatttcatc caccattgcc caatattctg atgcttgtaa atttgtattg ataatgcctc
     4021 ctttactgct tattttgatt gaaaaattgt tgcaaacatt ttcgattatt tcgtggacgt
     4081 tggcttcttt aaatttcatg atagatttct ctttttccaa gatggatgtt tctagaacct
     4141 tttctacctg aaaacttaat cgttgtgttt catcgtatat tatattagaa atgct
//
LOCUS       DAOPUR010000069         4172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21499_length_4172_cov_2.879767, whole genome shotgun sequence.
ACCESSION   DAOPUR010000069 DAOPUR010000000
VERSION     DAOPUR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21499_length_4172_cov_2.879767"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PKN90_01840"
     CDS             <1..876
                     /locus_tag="PKN90_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85052.1"
                     /translation="YSRLNNIANQIYACPKAFELIPRPAEYQAEISEAKKKAAELLYN
                     LGETALSYRTLEQSRIAYNHFNRANEYVYGYKDVFNKIEESLYYATLRVLVERPITAG
                     KYQLSAEFFSNNLIAEMTRQSQQKFIRFYTIEEAQSLKMNNPHQFLIFDFTDYTIGNV
                     RETIKTQELKRDSVVVGKVNVDGKSYNAYNTVYAKFTTNRREILSAGVLSVRIIDAPT
                     NRILQHQNFVGEYVWYTEWSSYSGDERALSDKQKRMTQLQPQLPPPHQDLFIEFTKPI
                     YSQVLTFVRSAYRNY"
     gene            893..1576
                     /locus_tag="PKN90_01845"
     CDS             893..1576
                     /locus_tag="PKN90_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85053.1"
                     /translation="MHKYIGMKKGRFVLLSLLLSLFTYNILAQHDAGYKKYIPSYDIS
                     VAIGDPMLASFYTDGYFLFRPYYDPKPFSFDDYVFGPEEYAGNTYITPVISTKITYQA
                     ASWYKVGGVFGYFGYYAKKHDVFTDAIVRRENTHVFLLMPEMMLLWLNESIIQIYSGA
                     GLGIAITMKEKLFTNEPSKNGLELSLLPTYHLNIIGARFGKELFGFVEYGVGFKGMFS
                     AGIGYKFVK"
     gene            1597..2430
                     /locus_tag="PKN90_01850"
     CDS             1597..2430
                     /locus_tag="PKN90_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85054.1"
                     /translation="MKHLSVLYTLFLLFILFSFSSCIYENQREPQPTQSGKAIYDSWH
                     THMFTVLQGDILKALHFSYYLDTQESQKIAVEDSLFSLFKIRDLSNGVWGLFLGADKI
                     YDINTSTTSLYHVGTEWIFKRTVSSSNHPLFSNSEIAYTITCIADSTWNISAVGDGNP
                     FCYVSLQLQQLGPTQFDVSGEGRFGIQSLNSYYYETENESNTPVVFLSFTLEETAKSR
                     NNFQNWSHGIISLRAYKLDGNERVTQVQMLNPSGDVYAVKVNYKGVSKVWSLSPNYYY
                     Y"
     gene            complement(2438..3043)
                     /locus_tag="PKN90_01855"
     CDS             complement(2438..3043)
                     /locus_tag="PKN90_01855"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNZ85055.1"
                     /translation="MIIKIHPQNPQEKEIKRVVDILENGGIIIYPTDTLYAFGCSIFD
                     QKAVNKICQLKQIDPKKVPLSFVCSTISHISEYAKISNDTFALLKNNLPGAFTFLLQG
                     NTNLPRLFKEKKTVGVRIPDNKIVKKIVETLNHPILSTSIFIDEDDTEYATNPDLIDE
                     KYGHLVDCVIDGGYGTLDVSTIVDCTEDSFEIIRQGKGILR"
     gene            complement(3098..>4172)
                     /locus_tag="PKN90_01860"
     CDS             complement(3098..>4172)
                     /locus_tag="PKN90_01860"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85056.1"
                     /translation="RSITVTGNVTLSATFAIKQYTVSLTAGTGGSVSGAGNYNHGQSV
                     TITATPTTGYNFVKWSDNVTTASRTFNATENLTLTATFAIKQYTVSLTAGTGGSVSGA
                     GNYNHGQSVTITATPSTGYNFVKWSDNVTTASRTFNASENLTLTATFAIKQYTVTLTA
                     GTGGSVSGAGNYNHGQSVTITATPSAGYDFVKWSDNNTNASRSITVTDNISLTAQFST
                     IQYTISTEIIEGGHITGGGNYPYGTKVTLVAVPDEDYTFYVWNDGHTSASREITVTED
                     KTYIAYFEEKVYTEVVEKQPTEYIVYSKNQTIFVEAPDGSSILIKDMMGRLLAKNTPT
                     LFVRNPGIYFIHINDTIVKLIVN"
BASE COUNT         1282 a          672 c          804 g         1414 t
ORIGIN      
        1 tattcacgtc ttaataatat cgcaaatcag atttatgcat gtcctaaagc atttgagctt
       61 attcctcgtc ctgctgaata tcaggctgaa atttcggaag caaaaaagaa agcagctgaa
      121 ttattataca atttaggtga aacagctttg tcgtatcgta cactagaaca atctcgcatt
      181 gcatataacc atttcaatcg agctaacgaa tatgtatatg gttataaaga tgtttttaat
      241 aaaatagaag agtcattgta ttatgcaaca ttacgagtat tggtagaaag accaattact
      301 gcagggaaat accagctttc ggcagaattt tttagtaata atttgattgc agaaatgacg
      361 cgtcaaagtc aacagaagtt tattcgtttt tatactatcg aagaggctca aagtttgaaa
      421 atgaataatc cgcatcaatt tttaattttt gattttactg attatacgat tggtaatgtg
      481 cgtgaaacta tcaagacaca agaactaaaa cgagatagtg tggtagtagg aaaagtaaat
      541 gtagatggga aaagttataa cgcatataat accgtatatg caaaatttac taccaataga
      601 agagaaattt tatcagctgg agtgttgagt gttcgtatca ttgatgcacc aactaatcgt
      661 attttgcaac atcaaaattt tgttggagaa tatgtatggt ataccgaatg gtcaagttat
      721 agtggggatg aaagagctct ttcggataaa caaaaacgta tgacacagtt gcaaccacaa
      781 ctccctccac ctcatcaaga tttgtttata gaatttacaa aaccaattta ttctcaggtg
      841 cttacttttg tgcgttcggc ttacagaaat tattaatact tgaagtgtat gtatgcataa
      901 atatataggg atgaagaagg gtagatttgt tcttttaagc ctgttattaa gtctttttac
      961 ttacaatata ctagctcaac atgatgcggg gtacaagaag tatattccta gctatgatat
     1021 ttctgttgct ataggcgatc caatgcttgc atcgttttat acagatggat atttcttgtt
     1081 tagaccatat tacgacccca aaccgttcag ttttgatgat tatgtatttg gacctgaaga
     1141 atatgcgggt aacacatata ttactcccgt aatctcaaca aaaattacct accaggctgc
     1201 atcatggtac aaagtaggtg gggtatttgg ttattttgga tattatgcta aaaagcatga
     1261 tgtatttacg gatgctattg tacgaagaga aaatacacat gtctttttat tgatgcctga
     1321 aatgatgttg ttgtggctca atgagtcaat tatccaaata tatagtggag cgggacttgg
     1381 aatagctata acgatgaaag agaagctatt tacgaatgaa ccatcaaaga atggtttaga
     1441 gttgagtttg ttgcctactt atcatctaaa cattattggg gcacgttttg ggaaggaatt
     1501 atttggtttt gtagagtatg gagtcggttt taaaggcatg ttttctgctg gtattggata
     1561 taagtttgta aaataattta aattaaacaa ttagttatga aacatttatc tgttttatat
     1621 acattattcc ttttgtttat actgttttca ttttcgagtt gtatttacga gaatcaacgc
     1681 gagccacagc ctacgcagag tgggaaagct atatatgata gttggcatac tcatatgttt
     1741 actgttttac aaggagatat tctcaaagca ttacactttt cttattatct agatacacaa
     1801 gaaagtcaaa aaatagccgt agaagatagt cttttctctc tgtttaagat tagagatttg
     1861 tcaaatggtg tgtgggggtt gtttttgggt gcagataaga tttatgacat aaatacatca
     1921 actacatcat tgtatcatgt tggtactgaa tggatattta aacgtactgt ttcttcgagt
     1981 aatcatcctc ttttttctaa ttcagaaata gcatatacaa ttacctgcat agccgattca
     2041 acatggaata tttctgctgt tggagatggg aatccttttt gctatgtgtc attacaatta
     2101 cagcaactgg gaccaacaca gtttgatgtt tctggcgagg gtcgttttgg tattcaatct
     2161 ctaaatagtt attattatga aacagagaac gaatcaaata ctccagttgt ttttttatct
     2221 tttactcttg aagaaacagc taaatcacgc aataattttc aaaactggtc tcatggtatt
     2281 atttctctaa gagcatataa gctagatggg aatgaaagag taacgcaagt tcagatgtta
     2341 aatcctagtg gagatgtgta tgctgtaaaa gtgaattata aaggggtttc aaaagtatgg
     2401 tctctaagcc caaactatta ctattattga gggtaaacta gcggagtatt cctttccctt
     2461 gacgaataat ctcaaagcta tcctcggtgc aatctacgat ggttgaaacg tctagtgttc
     2521 cgtatccacc gtcaataaca caatctacta agtgtccata tttttcatca attaagtctg
     2581 gattggtggc atattctgtg tcatcttcat caataaatat cgaagtagat aaaatgggat
     2641 gatttaaggt ttcgacaatt ttttttacaa ttttattgtc agggattcgg actccaacag
     2701 tctttttttc tttaaataaa cgaggtaaat ttgtattccc ttgtaacagg aaagtgaaag
     2761 cccctggtaa gttattttta aggagagcaa aggtatcgtt ggatattttt gcatattcac
     2821 tgatgtggct tattgtacta catacgaaag ataaagggac cttctttggg tcaatttgtt
     2881 ttaattgaca aattttattt actgcttttt gatcaaaaat actacatcca aaggcatata
     2941 gtgtgtcggt agggtagata ataatccccc cattctctaa aatatctacc acccgtttta
     3001 tctctttttc ttgcgggttt tgaggatgaa ttttaataat catacgaggc aatatagttt
     3061 agaggagtgg gagtaaaacc gctcctctaa gttgaattta gttaacaatt aatttaacaa
     3121 tagtatcatt gatgtgtata aaatagatac caggatttcg aacaaacaat gttggcgtat
     3181 ttttggctaa taacctaccc atcatatctt taataagaat gctgctccca tctggtgctt
     3241 ctacaaagat tgtttgattt ttgctgtaca cgatgtattc tgtcggttgt ttttcaacaa
     3301 cttctgtata aactttttct tcaaaatagg caatataggt tttgtcttct gttacggtaa
     3361 tttcacgact agcagaagtg tgaccatcat tccatacata gaaagtataa tcttcgtcag
     3421 gaactgcaac taatgtaact tttgttccat aaggataatt tcctcctcct gtaatatgac
     3481 ctccttcgat gatttccgtt gaaattgtgt attgtattgt actaaattgg gctgttaagg
     3541 aaatattgtc ggttacggtg attgaacgag aagcattggt attgttatcg ctccacttca
     3601 caaaatcgta tccagcactt ggtgttgctg taatggttac tgactgacca tgattgtaat
     3661 ttcctgcacc cgataccgaa cctccagtac ccgcagtcaa ggttacggta tattgtttga
     3721 tagcaaaggt cgcagttagt gttaggtttt cgctagcatt gaaagttcta cttgctgtgg
     3781 taacattatc actccacttc acaaaattgt accctgtact aggtgtagcc gtaatcgtta
     3841 cggattgacc gtgattgtaa ttccctgcac cagataccga acctccagtt cctgctgtaa
     3901 gcgaaacggt gtattgcttg atagcgaaag ttgcggtaag tgttaggttt tcagttgcat
     3961 taaatgttct gcttgctgtg gttacattat cgctccactt cacaaaattg tacccagtag
     4021 taggtgtagc cgtaatcgtt acggattgac catgattgta atttccagca ccagatactg
     4081 aaccaccagt accagcagta agcgatacgg tatattgctt gatagcaaaa gttgcggaaa
     4141 gtgttacatt gcctgttaca gtaatagaac ga
//
LOCUS       DAOPUR010000070         4171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21503_length_4171_cov_3.221574, whole genome shotgun sequence.
ACCESSION   DAOPUR010000070 DAOPUR010000000
VERSION     DAOPUR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21503_length_4171_cov_3.221574"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PKN90_01865"
     CDS             <1..758
                     /locus_tag="PKN90_01865"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNZ85057.1"
                     /translation="IFVLSLFFVTSTFLSCTKEERVPSSVISEEKMVDILVDVHMVEA
                     MARHNQLKDNNQRANMYYNYILEKHNITLNQLDSSISWYAKHPTTYIRVYDQIIPRLE
                     HSLEMVRTNANTGGQALRTLDIWEKKNEYQLTSLQNSNVPFRIEENSTIRFSLGDEFV
                     LKTKFSCKNVPSSLQLVMQVKIHFTDSTESIFEKKIVTTNPDFEQILRIQPDSKKQIA
                     MISGDLCYLIDKIDNPIPIEIRIFNVEMEQITK"
     gene            829..1134
                     /locus_tag="PKN90_01870"
     CDS             829..1134
                     /locus_tag="PKN90_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85058.1"
                     /translation="MIVLNVSLCVENILPLNHETPSTQFYNGIIFSLQNTPKDSIEDI
                     IQWQTNNSGKTIYDYLSNMGAQPIKIGTHLSELYVLLDIDFVSSIVTAHSSIRKVVS"
     gene            complement(1115..1867)
                     /locus_tag="PKN90_01875"
     CDS             complement(1115..1867)
                     /locus_tag="PKN90_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ85059.1"
                     /translation="MFMLSKSKIKFIRSLRSKKQRNDLRLFIAEGQKTVDDLLLYFPC
                     SMLFHTSEYCTSHSVSYLQEVTPEELKQISFLEEPQQVLGVFVMPEPIWSNDYLEGEL
                     IIALDMIQDPGNLGTILRVADWFGIKHIVCARGTVDVFNPKTIQATMGAIGRVHVYIV
                     DMGEFLQQLPHNIPVYGTFLEGENIYSTNVQNEAVIIMGNEGNGISQAIEKHVTHRIT
                     IPSFPPNQSTSESLNVAMATAIVCAEFRRRLF"
     gene            1955..>4171
                     /locus_tag="PKN90_01880"
     CDS             1955..>4171
                     /locus_tag="PKN90_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNZ85060.1"
                     /translation="MSRVIRYISLLIIVLLSACSATKYVPENKYLLDNVRIKSDIKNF
                     KSTELKTFLRQTPNSKMFDLISFNLGIYNLSGNNTDVWINRFLKRIGNPPIIFDSTLT
                     ERSEVELQRFFKNKGFENAQVESSVVFNKRKAKVTYNIISNNPYVINTIEYRIQDPII
                     KDLIEKDSSSSLIRKGNLFDVDVLDNERVRITRLLRNNGYYYFNKEYVRFLADSSLNT
                     NSVDVIIRIRKMERTGKRNTVLRSNHEVFSINSITYILVNDVLDINGYTNQKTKKDTL
                     FYKDKRIIYNNLFINPSILDDNTFITIGDNYSEYDTEKTYRRINNLHVSQFLRIDFSE
                     TPLLSDSIRKLDCTILLTPSNNQSFAIEAEGTNSAGDFGIAGKFGYTHRNAFTGGEKF
                     GLQLRASNEAIGSIRNILSYSAWEIGGETTLQFPRLMFPFVDRDFQRNSNANTEFNLN
                     YSYQTRPDFARTIAGIGLKYRWDSYRQSNMFHTLDFLDLSYVYLPPYSISDEFRNKYL
                     NSSTLLKYSYEDHLILRTGYSFSYTSKLLQQRRNGFSAKAGIETAGNSFYGIASLLKT
                     PKQENSYVIENIKFSQYIKLDGEYSYTQFLDPSNRIVYHIAMGIGIPYGNSSILPFEK
                     RYYGGGANSVRGWQVRTLGPGSYNNNGITDYMSQSGDMRADFNIEYRTKLVWLLELGA
                     FIDAGNVWTLLPYGEQVGGNFDINNIYREIALGYGIGIRFDFSYFLLRLDWGIKAYD"
BASE COUNT         1476 a          659 c          651 g         1385 t
ORIGIN      
        1 ttattttcgt tctttctcta ttttttgtaa ctagtacatt tctttcttgt acaaaagaag
       61 aaagagttcc ttcttctgtt atttctgaag aaaaaatggt tgatatattg gttgatgtac
      121 atatggtaga agcgatggct cgtcataacc aattaaaaga caacaatcaa cgtgcaaata
      181 tgtactacaa ttatattctt gaaaaacaca atattacact taatcagctt gatagctcta
      241 tttcttggta tgccaaacat cccactacct atattagagt atatgatcaa atcattccaa
      301 gactcgaaca ctcgttggaa atggttagaa caaatgcaaa tacgggtgga caagctttaa
      361 gaaccttaga tatctgggag aaaaaaaatg aatatcaact tacatcccta caaaatagca
      421 atgttccttt tcgaattgaa gaaaactcta ctattcgttt ttcacttgga gatgaatttg
      481 ttttaaaaac aaaattttcg tgtaaaaacg taccatcttc tttacaacta gtaatgcaag
      541 ttaaaattca ctttactgac tctacagaat cgatttttga aaaaaaaatt gtaactacaa
      601 accccgactt tgagcaaatt ttaagaattc aacccgactc aaaaaaacaa atagcaatga
      661 tcagtggaga tttatgttat ttgatagata aaatagataa tccaattcca atagagattc
      721 gcatttttaa tgtagaaatg gagcaaatta cgaaatagca atgtgtgcat gaaacgaata
      781 gcagcccatt atatagttgt ttctaataaa agaatattaa aaaaacacat gattgtttta
      841 aacgtttcct tatgtgtaga aaacattctt ccattaaatc acgaaactcc ttctactcaa
      901 ttttataatg gaattatttt ttccttacag aacacaccga aggattcaat agaggatata
      961 atacaatggc aaacaaacaa tagtggaaaa acaatatacg attatttaag taacatgggg
     1021 gctcaaccta ttaaaattgg cactcaccta tctgaattat atgttcttct tgatatagat
     1081 tttgtttcat ctattgttac ggcacactct tctattagaa aagtcgtctc ctaaattctg
     1141 cacaaactat tgcagtagcc atagccacat taagcgattc agaagtactt tgattgggag
     1201 gaaaagatgg aatagtgatt ctatgagtaa catgtttttc tattgcttgt gatataccat
     1261 tcccttcatt tcccataatt ataacagctt cgttctgcac atttgtagag taaatatttt
     1321 ctccttcaag aaaagttcca taaacaggaa tgttgtgagg taattgttgc aaaaactcac
     1381 ccatatctac aatatataca tgtaccctac caatcgcacc catagttgct tgtatcgttt
     1441 taggattaaa gacatccact gtaccacgag cacacacaat atgcttaata ccaaaccaat
     1501 ctgctacacg aagtatagta cctaaattac caggatcttg tatcatatct aacgctatta
     1561 tcaattctcc ttctaaataa tcattagacc atattggttc aggcattaca aaaaccccta
     1621 acacctgttg aggttcctct aaaaaactta tttgttttaa ttcttcaggt gttacttctt
     1681 gtagataaga aacgctatga ctagtgcaat actcgctcgt atgaaacaac atagaacatg
     1741 gaaagtataa caataaatca tctactgttt tttgcccttc agcaataaat aatcgtaggt
     1801 catttcgttg ctttttagaa cgtaaagaac gtataaactt tattttactt ttacttaaca
     1861 taaatattga gaataattat tatttttatc atctattgcg caaaagtaat tgttattatt
     1921 ttaattttgt ggtaaatttg tgcaaaatta agttatgagt cgtgtcattc ggtatataag
     1981 cctgctaatt atcgtattac taagtgcatg tagtgctacc aagtacgtac cagagaacaa
     2041 gtatttactt gacaacgtac gtattaaatc tgatataaaa aattttaaat caacggaact
     2101 gaaaacattc ttacgtcaga cacctaattc aaaaatgttt gatttaatta gttttaattt
     2161 aggtatttat aatttatcag gcaacaatac tgatgtatgg attaatcgtt ttttaaagcg
     2221 aattggaaat ccccccatta tttttgattc aacgttaaca gaacgttctg aagttgaatt
     2281 acaacgattt tttaaaaaca aaggctttga aaatgcacaa gttgaatctt ccgtcgtttt
     2341 taataaacgt aaagcaaaag tcacttacaa cattattagt aataatccat atgtaataaa
     2401 taccatagaa tatcgtatac aagatcctat tataaaagac ttgatagaaa aagattctag
     2461 ttcttctctt atacgcaaag gaaatctatt tgatgtggat gtgttagata acgaacgtgt
     2521 tagaattact agattattac gcaataatgg gtactactat tttaataaag aatatgtgcg
     2581 atttttagcg gatagttcgc ttaatactaa ttctgtagat gttattatac gtatacgaaa
     2641 aatggaacgt acaggaaaaa gaaacactgt attgcgtagc aatcatgagg ttttttccat
     2701 taatagcatc acatatattt tagtaaatga tgtattggac attaacggat ataccaatca
     2761 aaaaacaaaa aaagacaccc ttttttataa agataaacga ataatttaca acaatttatt
     2821 tatcaaccca tcaatattag atgataatac gtttattaca attggggata attattcaga
     2881 atatgatacc gaaaaaacct atcgaagaat caataatcta catgtgtctc aattcttacg
     2941 cattgatttt agcgaaaccc ctttactctc tgatagtatc cgcaagttag attgtacaat
     3001 tttattaaca cccagtaaca atcaatcatt tgctattgaa gcagaaggga caaattctgc
     3061 tggtgatttt ggtattgctg gaaagtttgg ttacacacat cgtaatgcat ttacaggggg
     3121 agaaaagttt ggtttacaat taagagcatc aaacgaagct attggtagca ttcgtaatat
     3181 attatcatat agtgcttggg aaattggtgg tgaaactacc ttgcaatttc ctcgacttat
     3241 gtttcctttt gtagatagag attttcagcg taatagtaac gctaatactg aatttaatct
     3301 caattatagc tatcaaaccc gacctgactt cgctcgaacc attgctggaa ttgggttaaa
     3361 atacagatgg gatagctacc gccaatcaaa tatgtttcat acactagact ttcttgattt
     3421 aagctatgta tatttaccac cctattctat ttctgatgaa tttagaaata agtatctaaa
     3481 tagctctact ttattgaaat atagttatga agaccactta atcttacgta cagggtattc
     3541 tttttcatat acaagtaaat tattacaaca aagacgaaac ggtttttctg caaaagcagg
     3601 tattgaaact gctggaaata gtttttatgg aatagcatct ttactcaaaa caccaaaaca
     3661 agaaaatagt tatgtaatag agaacattaa attttcgcaa tacataaaac tagatggaga
     3721 atatagttat actcagtttc ttgatcccag caatcgaata gtatatcata ttgcaatggg
     3781 tattggaatt ccttatggaa atagttccat tttacctttt gaaaaaagat actatggcgg
     3841 gggtgctaat agcgtaagag gatggcaagt acgtacatta ggacctggtt cttataacaa
     3901 taatggaatt acagattata tgagtcaatc tggcgatatg cgtgctgatt ttaatattga
     3961 atatagaaca aaactagtat ggttgttaga gttaggagca tttattgatg caggtaatgt
     4021 gtggactctt ttaccatatg gagaacaagt gggtggcaat tttgatatca ataatatata
     4081 tagagaaatt gcactaggtt atggaatagg aattcgattc gatttctctt attttttatt
     4141 acgtttagac tgggggatta aagcatatga c
//
LOCUS       DAOPUR010000071         4164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21558_length_4164_cov_2.916768, whole genome shotgun sequence.
ACCESSION   DAOPUR010000071 DAOPUR010000000
VERSION     DAOPUR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21558_length_4164_cov_2.916768"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PKN90_01885"
     CDS             <1..613
                     /locus_tag="PKN90_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ85061.1"
                     /translation="PQGVQVQVGGDYETQQESFADIATLFLLIVILVYIVMASQFESF
                     SKPFIIMMSVPFAITGVILALLITGTSLNLIGALGVVLLVGIVVKNGIVLVDYINLMR
                     DRGNELNEAIALSGQSRLRPVLMTAVTTILGMIPMTLSTSEGSETWVPMGIVVIGGLV
                     VSTLVTLIVVPTLYAVMSRRGERNKEEKLRKSFTFFDKPVSKE"
     gene            644..937
                     /locus_tag="PKN90_01890"
     CDS             644..937
                     /locus_tag="PKN90_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85062.1"
                     /translation="MKSVFIVFNQANTERVEYMLDRLSIRGFTFWENVQGVGSMNGEP
                     RRGTHTWPEMNSAVMTVIPDEKVDDLLIAVKKLDQRNKEVGVRAFVWNIEQMV"
     gene            complement(942..2000)
                     /gene="mutY"
                     /locus_tag="PKN90_01895"
     CDS             complement(942..2000)
                     /gene="mutY"
                     /locus_tag="PKN90_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNZ85063.1"
                     /translation="MTFSEIIIDWYEVNKRNLPWRNTKDAYTIWVSEVILQQTRVVQG
                     LAYFIRFIERFPTVIALAQADEEEVLKYWQGLGYYSRARNMHKAAQQVVELYDGIFPT
                     HYPHLLTLQGIGEYTAAAISSFAANEAHAVVDGNVYRVLSRVFGIFLAINSNEGKREF
                     KKLAQSLLPTHQVSTYNQAIMEFGALQCVPARPLCSTCPLQVMCYAYTHQHVSSLPKK
                     EKKTTTKTRYFFYLDIRNGEYTYLQKRTQKDIWHNLFEYPLVECDKETSIENVINSED
                     FQEIVEKCEKTSIHSISSEIKHVLSHQIIRTIFIRIHIETDNSLLQSLYKINSRDIED
                     YPISRLMHKYIEKITNET"
     gene            2163..2438
                     /locus_tag="PKN90_01900"
     CDS             2163..2438
                     /locus_tag="PKN90_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ85064.1"
                     /translation="MTKAEIVNEIANKSGIDKVTVLRVVESFMDEVKDSLASNENVYL
                     RGFGSFVVKQRAEKVARNISRNTTITIPAHSIPSFKPAKTFVEAVKK"
     gene            2667..>4164
                     /locus_tag="PKN90_01905"
     CDS             2667..>4164
                     /locus_tag="PKN90_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNZ85065.1"
                     /translation="MNNTLLLDVQSKDISIALMENNTLVEFSKEPRDMSFSVGDIFLA
                     KVKKIMPGLNATFVDVGYDKDAFLHYLDLGSNFHSIAQFTKTLVNDRKKIPSMQKNSG
                     LHDLEKDGFIADILKSGQEILVQITKEPISTKGPRLTAEISIAGRSLVLLPFSNKISI
                     SQKIKSEEERNRLRQLLVSIKPKNFGVIIRTVAEGKKVAELDAELKILEKRWEEAIEN
                     IQKAKQFPSLILKEMSRAETILRDSFNPNFQHIYVNEKTIFEEIQAYVGLIAPEKNDI
                     VKLYTDDIPIFDAFNITKQIKSSFGKITPFKNGAYLVIEHTEALHVIDVNSGNRSKAG
                     NVQETNALEVNLAAVDEVARQLRLRDMGGIIVIDLIDMQENEHKQQVYERMKERMQND
                     SAKHNILPLSKFGLMQITRQRVRPQTLIDTKETCPTCDGNGKIRSSLFFVDMLETKLN
                     EIKNTMKIRKCILFVHPFVYSFINAGIMSIKNKWILKFGIGFKVLPNQS"
BASE COUNT         1409 a          629 c          770 g         1356 t
ORIGIN      
        1 tccacaagga gtacaagtgc aagttggtgg tgattatgaa acacaacagg aatcgtttgc
       61 tgatattgct accttgtttt tattgattgt aattttagta tacattgtaa tggcatcgca
      121 gtttgaatct ttctccaaac catttataat tatgatgtcc gttccttttg ctattacagg
      181 tgttattttg gcattgctga ttaccgggac atcacttaat ttaatagggg cattaggtgt
      241 ggtactattg gtaggtatcg tggtaaaaaa tggtattgtg ttagtcgact atatcaattt
      301 aatgagagat agaggaaatg agctaaatga agctattgct ttatcagggc agtcacgttt
      361 acgaccagtg ttaatgaccg ctgtaactac tattttgggg atgattccga tgacattaag
      421 cactagcgag ggctctgaaa catgggtgcc tatggggatt gtagtaattg gaggtctggt
      481 tgtttctacc ttagtaactc ttatcgtagt tccaacattg tatgctgtga tgtctcgtag
      541 aggagaacgc aataaagaag aaaagttacg taaatcattt acgttctttg ataaaccagt
      601 gagtaaagaa tagttaaaat taccagaaaa taaaatacac catatgaagt cagtatttat
      661 tgtgtttaat caagctaata cagaacgtgt agaatatatg cttgatagat tatccattag
      721 aggctttacc ttttgggaaa atgtgcaagg agtaggcagt atgaatggag aaccgcgtcg
      781 tggtacacat acttggccag aaatgaattc tgcagttatg acagtcattc ctgacgaaaa
      841 agtagatgat ttattgattg ctgttaaaaa actagatcaa cgtaataaag aagtaggcgt
      901 tagagcattt gtttggaata ttgagcaaat ggtatagtta cttaggtttc gttagtaatc
      961 ttttcaatat acttatgcat cagtcgagat ataggatagt cttctatgtc tcgactattt
     1021 attttatata atgattgtag tagtgaattg tcagtttcta tgtgtatacg gataaaaata
     1081 gtacgtataa tttgatgact tagaacatgt tttatttcac ttgagatact gtgaatagat
     1141 gttttctcac atttttctac tatttcttga aaatcttcac tattgataac gttttctata
     1201 gatgtttcct tatcacattc tactagtgga tattcaaata aattatgcca aatatctttt
     1261 tgtgttcttt tttgaagata cgtatattca ccattacgta tatccaagta aaaaaaataa
     1321 cgtgttttcg ttgtagtttt tttctctttt ttgggtaaag aacttacgtg ttgatgggta
     1381 tatgcataac acataacttg caatggacaa gttgaacaca atggacgagc aggaacacat
     1441 tgcaacgctc caaactccat aatagcctgg ttataagtgc ttacttgatg tgtggggagt
     1501 aaagattgtg ctaatttctt aaattctctt ttgccctcgt tgctattaat agctaaaaag
     1561 ataccgaata ccctgcttaa taccctgtac acatttccat ctactacagc atgtgcctcg
     1621 ttggctgcaa aagagctaat agctgccgcg gtatattcgc caatgccttg taaggtaagt
     1681 aagtgggggt aatgagtagg gaatatacca tcatacaact ccacaacctg ctgtgcggct
     1741 ttgtgcatat tacgagctct cgaataatat cctaaccctt gccaatactt caaaacctct
     1801 tcttcatcag cttgtgctaa ggcaataaca gttggaaacc gttcgataaa acgtataaaa
     1861 taagcaagac cttgcactac cctcgtttgt tgtaaaataa cttctgatac ccaaatagtg
     1921 taagcatctt tggtattacg ccaaggtaaa ttccgtttat ttacctcgta ccaatctatt
     1981 attatttcac taaaagtcaa tgtcgtatat tatatgtagc aaaagtagct attttttcaa
     2041 attaaatcaa acattcaact ttttagatta tttttttttg aataatgtag attattgaaa
     2101 ctaaaagtat tatatttgca gtccgttttt aaaagaaaag tataactata aaatagacta
     2161 aaatgactaa agcagaaatc gttaacgaaa tcgcaaacaa atcaggtatt gataaggtta
     2221 ccgtgttgag agtagttgaa tcatttatgg atgaagtgaa ggattcgtta gcttctaacg
     2281 aaaatgtgta tttaagagga tttggtagct ttgttgtaaa acaaagagca gagaaagtgg
     2341 ctcgtaatat ttctcgtaat actaccatta ctataccagc acattcaatc ccatcattta
     2401 agcctgctaa aacttttgtt gaggctgtaa aaaaataaaa ttatttaatt gttataaaag
     2461 aatacggata tgccaagcgg aaaaaaaaga aaaagacata aaatgtcaac tcacaagcgt
     2521 aaaaaaagac taagaaaaaa tagacataaa aagaaaaaat gatttttttg tctttctaaa
     2581 acaactaagc acaataagaa caaacttaaa ttatctgttt tgagtttgtt ctttctgcat
     2641 accaatacat cacatttata tactatatga ataacacttt acttcttgat gttcaatcta
     2701 aagatatttc tatcgcattg atggaaaata atactttggt agaatttagc aaggagccac
     2761 gtgatatgtc tttttccgta ggagatattt ttttagcaaa agttaaaaaa attatgcctg
     2821 gattaaatgc cacgttcgta gatgtaggat atgataaaga tgcatttttg cactatttag
     2881 atttaggtag taatttccat tctattgcac agtttaccaa aacactggtc aatgacagga
     2941 agaaaatacc ttcaatgcaa aaaaattctg gtttacatga cttagagaaa gatggcttta
     3001 ttgctgatat tttaaaatct ggtcaggaaa ttttggtgca aataaccaaa gaacccatat
     3061 caactaaggg tcctcgacta acagccgaaa tatctatagc tggacgatca ttagtattat
     3121 tgcctttttc aaataagatt tccatttctc aaaaaattaa atcggaagaa gaacgcaatc
     3181 gacttcgtca attattagtt agcataaaac ccaaaaattt cggtgttatt attcgcactg
     3241 ttgctgaagg caaaaaagtg gcagaattag atgctgaact gaagatttta gaaaaaagat
     3301 gggaagaagc tattgaaaat attcagaaag ccaaacaatt tccatcctta atcctcaaag
     3361 aaatgagtag ggctgaaact attctacgtg atagttttaa tccgaatttt caacacattt
     3421 atgtgaatga gaaaactatt ttcgaagaaa tacaagctta tgtaggtttg atagctcccg
     3481 aaaaaaatga tattgtaaag ctatataccg atgatattcc aatttttgat gctttcaata
     3541 tcaccaagca aattaaatcc tcattcggta agattactcc tttcaaaaac ggtgcctatt
     3601 tggttattga gcatacagag gcgttgcatg taattgatgt aaatagtggg aatcgatcta
     3661 aagctggtaa tgttcaggaa accaatgcgt tagaggttaa tttagctgct gttgatgagg
     3721 tagcaaggca attacgatta cgtgatatgg gtggtattat agttattgac ttgattgata
     3781 tgcaagaaaa tgagcataag caacaagtgt acgaacgcat gaaggagcgg atgcaaaacg
     3841 attctgcaaa acataatata ttaccattaa gcaagtttgg tttaatgcaa attacacgcc
     3901 aacgtgttcg accacagacg cttatagata cgaaagaaac ctgtccaacg tgtgatggaa
     3961 atggtaaaat ccgttcttcc ctgttttttg tggatatgtt ggagacaaaa ttaaatgaaa
     4021 ttaagaatac tatgaagatt cgaaaatgta ttctttttgt acatcctttt gtgtatagtt
     4081 ttattaatgc gggtatcatg agtattaaga ataaatggat actcaagttt ggcataggtt
     4141 ttaaagtgtt acccaatcaa agct
//
LOCUS       DAOPUR010000072         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21653_length_4149_cov_3.585491, whole genome shotgun sequence.
ACCESSION   DAOPUR010000072 DAOPUR010000000
VERSION     DAOPUR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21653_length_4149_cov_3.585491"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /gene="rplE"
                     /locus_tag="PKN90_01910"
     CDS             complement(<1..442)
                     /gene="rplE"
                     /locus_tag="PKN90_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNZ85066.1"
                     /translation="MSNKASLKKEYTERIAPALKKEFGYKSAMQVPVLEKIVLNQGLG
                     IAVADKKIIEIAINEMTAIAGQKAIATYSKKDISNFKLRKKMPIGVRVTLRREKMYEF
                     LERLVRVALPRIRDFKGIDSKLDGRGNYTLGIQEQIIFPEINIDS"
     gene            complement(442..756)
                     /gene="rplX"
                     /locus_tag="PKN90_01915"
     CDS             complement(442..756)
                     /gene="rplX"
                     /locus_tag="PKN90_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNZ85067.1"
                     /translation="MTKLHIKKGDTVYVNAGDDKGTVARVLEVLVKEQRAIVEGVNMV
                     SKHTKPNAKNPQGGIVKKEASIHISNLNPTVKGDKPTRVGRKQVDGKLVRYAKKTGEE
                     IK"
     gene            complement(777..1142)
                     /gene="rplN"
                     /locus_tag="PKN90_01920"
     CDS             complement(777..1142)
                     /gene="rplN"
                     /locus_tag="PKN90_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNZ85068.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGGTGKRYATLGDVIVVAVK
                     SVIPSSDVKKGAVSKAIIVRTKKEVRRADGSYIRFDDNACVLLNAAGEMRGSRIFGPV
                     ARELRTANMKIVSLAPEVL"
     gene            complement(1144..1395)
                     /gene="rpsQ"
                     /locus_tag="PKN90_01925"
     CDS             complement(1144..1395)
                     /gene="rpsQ"
                     /locus_tag="PKN90_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNZ85069.1"
                     /translation="MRNLRKERQGVVFSNKMDKTITVAIKWKEKHPIYGKFVNKTKKY
                     HAHDEKNECTIGDTVKIMETRPLSKLKRWRLVEIIERAK"
     gene            complement(1408..1605)
                     /gene="rpmC"
                     /locus_tag="PKN90_01930"
     CDS             complement(1408..1605)
                     /gene="rpmC"
                     /locus_tag="PKN90_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNZ85070.1"
                     /translation="MKIAEVKELSNQEIIERIDGEKEKLSQLKLNHSISPLDNPNQIK
                     EVRKTIARLNTELRAREMKQN"
     gene            complement(1612..2046)
                     /gene="rplP"
                     /locus_tag="PKN90_01935"
     CDS             complement(1612..2046)
                     /gene="rplP"
                     /locus_tag="PKN90_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNZ85071.1"
                     /translation="MLQPKKTKFRRQQKGRMKGNAQRGNQLAFGSFGIKSLESKWITG
                     RQIEAARIAVTRYMQRQGQIWIRIFPDKPITKKPAEVRMGKGKGSPEGFVAPITPGRV
                     IIEVEGVPFEIAKEALRLAAQKLPVTTKFVTRRDYDYESPNA"
     gene            complement(2075..2812)
                     /gene="rpsC"
                     /locus_tag="PKN90_01940"
     CDS             complement(2075..2812)
                     /gene="rpsC"
                     /locus_tag="PKN90_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNZ85072.1"
                     /translation="MGQKVNPISNRLGIIKGWDSNWYGGNNYGDTILEDFKLRKYLEA
                     RLAKASVSRIIIERTLKLITITVCTARPGIIIGKGGQEVDKLKEELKKITDKDVQINI
                     FEIKKPELDAVIVANNVARQIEGKIAYRRAVKMAIASTMRAGAEGVKIMVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYAHAEALTKVGIIGVKVWICRGEIYGKKDLTPQFAT
                     QKEGARPQFGNANRSENAGKGFKKRKK"
     gene            complement(2819..3229)
                     /gene="rplV"
                     /locus_tag="PKN90_01945"
     CDS             complement(2819..3229)
                     /gene="rplV"
                     /locus_tag="PKN90_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNZ85073.1"
                     /translation="MGARKKISAEENKEALKGLYFAKLNNVPTSPRKMRLVADMIRGV
                     EVHKALGLLKFSTKEAAGRLEKLLRSAISNWEQKTEQKAEDANLIVSRIHVDSGRMLK
                     RLRPAPQGRGYRIRKRSNHVTIYVDTVNTNDTKN"
     gene            complement(3269..3538)
                     /gene="rpsS"
                     /locus_tag="PKN90_01950"
     CDS             complement(3269..3538)
                     /gene="rpsS"
                     /locus_tag="PKN90_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNZ85074.1"
                     /translation="MSRSLKKGPFINAKLDKKVTALNEANKKNVIKTWSRASMISPDF
                     VGHTIAVHNGNKFIPVYVTENMVGHKLGEFSPTRNFRGHGGNKKK"
     gene            complement(3568..>4149)
                     /gene="rplB"
                     /locus_tag="PKN90_01955"
     CDS             complement(3568..>4149)
                     /gene="rplB"
                     /locus_tag="PKN90_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNZ85075.1"
                     /translation="EYDPNRSARIALLFYADGEKRYIIAPNGLEVGQTVLSGVDAAPE
                     IGNALPLQNIPLGTIVHNIELNPGQGAALVRSAGTFAQLTSREGKYAIVKLPSGETRM
                     ILCTCKATIGTVGNSDHALERSGKAGRSRWLGRRPRVRGVVMNPVDHPMGGGEGRASG
                     GHPRSRKGLLAKGYKTRAPKKASSKYIIERKKK"
BASE COUNT         1118 a          869 c          618 g         1544 t
ORIGIN      
        1 ttgaatcaat atttatttca ggaaaaataa tttgttcttg gatacctagc gtataattac
       61 cccttccatc aagtttacta tcaatacctt taaaatcacg tatacgaggt aaagctacac
      121 gaacaagtct ttcgagaaat tcatacattt tttctcttct taaagttacc ctaacaccaa
      181 taggcatttt ttttcttaat ttaaaattag aaatatcctt ttttgaatag gtagctatag
      241 ctttttgacc agcaatagca gtcatttcat taattgcaat ttcaataatt tttttatcag
      301 ctacggctat acctaaacct tgattcaaaa ctattttttc aagaactgga acctgcattg
      361 cagatttgta tccaaactct ttctttaaag caggagcaat acgctctgta tactcttttt
      421 taagacttgc tttgttactc attatttaat ttcctcccca gtttttttag cgtaacgtac
      481 caatttacca tcaacttgtt tgcgtcctac tcgtgtaggt ttatctcctt ttacagtagg
      541 atttaagttt gaaatatgga tagacgcctc ttttttaaca ataccacctt gtggattttt
      601 tgcatttggt ttagtgtgct tagacaccat attaacacct tctacgatag ctctttgttc
      661 ttttaccaaa acctcaagca cacgagcgac tgtacccttg tcatcacctg catttacgta
      721 aacggtgtct ccttttttta tgtgtaattt agtcattttg tatattgttc aaagggttat
      781 aaaacttctg gtgcaagcga tacaattttc atatttgctg tacgcaattc acgtgctacg
      841 ggaccaaata tacgactacc tctcatttca ccagcagcat ttaacaacac acatgcgttg
      901 tcatcaaaac ggatataaga cccgtcggct ctacgtactt cttttttggt acgtacaatg
      961 attgctttag aaacagcacc tttttttaca tcacttgatg gaattacaga ttttaccgct
     1021 acaacaataa catctccaag tgtagcatat cttttacctg ttcctcctaa tactcggata
     1081 caaagaactt cttttgcacc gctattgtct gcgacattca gtctactttc ttgttgtatc
     1141 atgttatttt gctctttcga ttatttctac taatctccat cttttaagct tgcttaaagg
     1201 acgagtttcc ataattttta ctgtatcacc aattgtacac tcattttttt catcatgagc
     1261 atggtatttt tttgttttat tcacgaactt accgtaaata ggatgctttt ctttccactt
     1321 gatagcaaca gtaatggttt tatccatttt attgctaaaa accacgccct gtctttcttt
     1381 tcttaaattt ctcatatcag gtcaagttta attttgtttc atttctcttg cacgtagttc
     1441 tgtgttcaga cgagcaattg ttttacggac ttctttaatt tgatttggat tatctaatgg
     1501 agaaatacta tgattcaatt ttaattgaga taatttttct ttttcgccat caattctttc
     1561 gataatctct tggttggata attcctttac ttcagctatt ttcatacctc attatgcatt
     1621 aggagattca taatcataat cacgtctagt aacaaatttg gtagttactg gcaatttttg
     1681 tgctgctaag cgtaatgctt ctttagcaat ttcgaaagga acaccttcca cttcaataat
     1741 tacacgacca ggtgtaatcg gggcaacaaa accctcaggg gatccttttc ctttacccat
     1801 acgcacctca gcaggttttt tagttattgg tttatctgga aaaatacgta tccaaatttg
     1861 accttgtctc tgcatataac gcgtaactgc aatacgagca gcttctattt gccttcctgt
     1921 aatccatttg gattctaaag actttattcc gaaagatccg aatgccagct gattgccacg
     1981 ttgggcatta cccttcatac ggcctttttg ctgtcttctg aactttgttt ttttaggttg
     2041 taacatgttt ccttatgtaa aattcgttaa cagattattt ttttcttttt ttaaaacctt
     2101 tgcctgcgtt ttcagatcga ttggcatttc caaattgagg acgagcacct tctttttggg
     2161 ttgcaaattg cggtgtcaaa tcttttttac catatatttc gccacgacaa atccacactt
     2221 taacgccaat tatacctact tttgttaaag cttcagcatg tgcatagtca atatctgctc
     2281 taaaagtatg taaaggagtt ctaccttctt tatacatttc cgaacgagcc atctctgcac
     2341 catttaaacg accagaaacc attatcttaa ctccctctgc tcctgcacgc atagttgatg
     2401 caattgccat tttaactgct cttctatagg cgattttacc ctctatttga cgagcaacat
     2461 tatttgcaac aatcactgca tctaactctg gttttttgat ttcaaaaata ttgatttgaa
     2521 catctttatc agtaattttt ttaagctctt ctttgagttt atcaacctct tgaccacctt
     2581 taccaattat gataccagga cgagctgtac atactgtaat agtgatgagt tttagtgtgc
     2641 gttcaataat gatacgagaa acactagcct tagctaagcg agcttctaaa tattttctaa
     2701 gtttaaaatc ttctaaaatt gtatcaccat agttatttcc accataccaa ttagaatccc
     2761 aacctttgat gattcctaaa cggttactta ttggatttac tttttgtccc atctactatt
     2821 aattttttgt atcgttagta tttacagtgt ctacataaat ggttacatgg ttcgagcgtt
     2881 tacgaattct gtatcctcta ccttgcggag ctggacgaag tcttttaagc attctacctg
     2941 aatccacatg tatgcgacta actattaagt tagcatcttc tgctttttgt tctgtttttt
     3001 gttcccaatt agaaattgca gagcgaagaa gtttttctaa acgaccggca gcttctttgg
     3061 tggaaaattt taacagtcca agcgctttgt gcacttccac gcctcttatc atatctgcaa
     3121 ctaaacgcat tttacgtgga gacgtaggca cattatttag ctttgcgaaa taaagaccct
     3181 ttaaagcttc tttattttct tctgctgata ttttttttct agcacccatt tttatactta
     3241 ttatttattc ctaatttata ttcaacggtt atttcttttt atttccacca tgacctctaa
     3301 aattacgagt tggcgaaaat tctcctagtt tatgtcctac catattttcg gtaacataca
     3361 ctggaataaa tttatttccg ttgtgtacag caattgtatg accaacaaag tctggagata
     3421 tcatagatgc tcttgaccat gttttgatca cattcttttt gttagcctcg tttaaggcag
     3481 tcactttttt atcaagttta gcgttgataa atggaccttt ttttaatgaa cgactcatat
     3541 tattatactt ctcttaaatt aatcaaatta ctttttcttt ctctcgatga tatatttact
     3601 tgaagctttt tttggagcac gtgttttata ccctttagct aacaaacctt tacgtgatct
     3661 tggatgacct ccagaagcac gaccttcacc accacccatt gggtgatcca caggattcat
     3721 gacaacgcca cgaactcttg gtcgtctacc taaccatctt gaacgacctg ctttacctga
     3781 acgttccaaa gcatgatctg aatttcctac tgttccgatt gtggctttgc atgtgcaaag
     3841 tatcatacgg gtttcaccag aaggcaattt aacaattgca tatttacctt ctctcgaagt
     3901 caactgtgca aaagttcccg cagagcgaac caaagcagct ccttggccag gattcaattc
     3961 aatgttgtgt acaattgttc caagaggaat attttgtaaa ggcaatgcat tcccaatttc
     4021 tggtgcagca tccactcctg acaaaactgt ttgacctact tctagaccat taggtgcgat
     4081 aatataacgt ttttctccat ctgcgtaaaa tagcaatgcg atacgtgccg aacgatttgg
     4141 atcgtattc
//
LOCUS       DAOPUR010000073         4137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21736_length_4137_cov_3.026213, whole genome shotgun sequence.
ACCESSION   DAOPUR010000073 DAOPUR010000000
VERSION     DAOPUR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21736_length_4137_cov_3.026213"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKN90_01960"
     CDS             complement(<1..638)
                     /locus_tag="PKN90_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ85076.1"
                     /translation="MRKNGSSLHPSPLFSISLRVICVITLLFLSHISVVAQQVFSISG
                     TISDKATGETLTGATIYPKSNPSSAVYSNAYGYYALSLAKGTHTIVVNYIGYSSLEYS
                     VEVQENIRKNFQIQPASTQLDEVVVSAIKRNETILSDKIGVEKLEIQSIQKIPVLFGE
                     QDILKTLTLTPGVKTMGEGSGGMYVRGGNNSHNLILLDESMVYNANHLLGFFS"
     gene            complement(667..738)
                     /locus_tag="PKN90_01965"
     tRNA            complement(667..738)
                     /locus_tag="PKN90_01965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(703..705),aa:Arg,seq:cct)
     gene            complement(798..1523)
                     /locus_tag="PKN90_01970"
     CDS             complement(798..1523)
                     /locus_tag="PKN90_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HNZ85077.1"
                     /translation="MLAKTRAIVIHTTKFNDKDLILHALTEEFGMVSYMIYNPWSKQA
                     KVKASYLQTFSLLEIDVEHQPKKDIQRIHDAKILFVGNSIHTNPYKQSIVLFLAEMVR
                     KTLSETEKDVHLFAFVYHSLCVLDEVTEKYNSFHLIFLMRLTQFLGFYPNSTVENNDV
                     YFDMLNGIFTAQPPLHGHYLEGENCKHFQALTHISYTDMPAWNISRVDKLTLLSTMVR
                     YYQLHIPTIQRIKSLNILHSLFV"
     gene            complement(1526..3727)
                     /locus_tag="PKN90_01975"
     CDS             complement(1526..3727)
                     /locus_tag="PKN90_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ85078.1"
                     /translation="MKQLLVVILAFFSIGTNYAQIQSGGFSPLPFTYSNEQHTIIGKR
                     NTQPLFYKIPYNGEQDNKEEKTSRFVVGKSFAVDYSPLNAGQQFTTEDGTTIWRIGFY
                     SPNAASISVVFDEFEIPEGASIFVYNPSQTNVAGAFTSINNNSNKSLPIAPFPSDSII
                     IEYQQPATVTFNGKLRIGRVTHNTIDFINRSTGSFNSAEKCSPHAAFVEGVSNIKQAT
                     CLLYVTDNSESWLGSGVMIKNSEGKPYLLTAAHNYEGTNLEQTTIFYFNYEVPKVDSL
                     IRGSQEFTLGGSVLKAKAYDIDFALVELNQMPPADYRPYLAGWNRANSDLAPMVCVQH
                     PQGDVKRVSTDNDNPLAVTYPYNDLLETKLADAHWKITRWEKGTTEPGSSGSGLFDNN
                     YRIIGGLTGGNSTCNSPVEDYFFRLNKAWSYYSSESKQLKRWLDPTNTNVMYMDGRNP
                     YEATPCIRYSNVQSADVMGKVSVGNGKTGRLSGHNELQYTEFAEKFVTDTNNYLYGVY
                     IVPFIGKYNVNTPVNVHVYNGTSSPETLLTTVLFNPKTLAYQATSQTFSQTTKTQFLN
                     RENYVRFPNPVLVNEEFFVSYSITYQPVDSFAVYMVNNRAIDALNTAYYKDNGIWKSF
                     TEHPYKPMASSLWIEPVFQEANESLIFYPHDEDANAILFPNPCDSEIQVFRKETNTAQ
                     FSLFSIQGTLLFTISSIENPLTINTSGLQQGIYFLQITRDDTSNTYKLIKK"
     gene            complement(3738..>4137)
                     /locus_tag="PKN90_01980"
     CDS             complement(3738..>4137)
                     /locus_tag="PKN90_01980"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNZ85079.1"
                     /translation="HWQQIHWRALEAGYNSSPFFEYYKDDFAPFYTNKFEFLVDYTFQ
                     LQSLVIALLELDCKVQLTEAYTTYDDALDYRDALSAKKEIEDNDFVISSYYQVFENKF
                     GFIPNLSILDLLFNMGNESILVLQKSASKH"
BASE COUNT         1245 a          723 c          768 g         1401 t
ORIGIN      
        1 gaaaaaaaac cgagcaaatg gttcgcgtta tataccatcg attcgtctaa aagaattaaa
       61 ttgtgcgaat tattaccacc tcgcacatac atgcctccac ttccttctcc catggttttt
      121 acgcctgggg ttaatgttag tgttttaaga atatcttgtt caccaaacag tacggggatt
      181 ttctgaatag attgtatttc aagcttttct acaccaattt tgtctgataa aatagtttcg
      241 tttcttttaa ttgctgatac gactacctca tccaattgtg tgctagctgg ttggatttga
      301 aaattctttc gaatattctc ttgtacctca acggagtact ctaggctgct atatccaata
      361 taatttacca caatagtatg agtgccctta gctagcgaaa gggcataata gccatatgcg
      421 tttgaatata cagcactaga aggattgctt tttggataaa ttgtagctcc tgttaatgtt
      481 tcacctgttg ctttgtcgga aatagtgcca ctgatagaaa aaacttgttg ggctaccact
      541 gaaatgtgtg aaagaaatag gagtgttatc acacagataa ccctaaggct gatagagaaa
      601 agaggagaag gatgaaggga agaaccgttt tttctcataa gtacatagcc tgaattaggg
      661 acatcgtacc tccgctggga atcgaaccca aatctaaagt ttaggaaact tttgttctat
      721 ccattgaact acagaggcaa aaatttgatg agcaaaagta ctattttttc cagtaagaaa
      781 caaactattt ttttacatca tacaaacaaa gaatggagaa tattcaagga ttttatccgt
      841 tgtatggtcg gtatatgcaa ttgatagtaa cgtaccatag ttgatagtaa tgtcaattta
      901 tctacacgcg atatattcca agcaggcatg tcagtatagc ttatgtgcgt aagtgcctga
      961 aaatgcttac agttttctcc ttctaaataa tgaccatgca aaggtggctg tgcagtaaat
     1021 attccgttta gcatatcaaa atacacatca ttattttcta ctgtactatt tgggtaaaaa
     1081 cctaggaatt gagttaatcg cattaagaaa ataaggtgaa aagaattgta tttttctgtt
     1141 acttcatcta atacacacaa cgaatgatag acaaaagcaa ataagtgaac gtctttttct
     1201 gtttctgata atgttttacg taccatttcg gctaaaaaga gaacaatact ttgcttgtaa
     1261 ggattggtat gtatgctatt gcctacgaat agtattttgg catcatgtat acgctgaata
     1321 tcttttttag gttgatgttc tacatcaatt tctagcagac taaatgtttg taggtacgat
     1381 gcttttactt tggcttgttt gctccatgga ttataaatca tgtacgatac catgccaaat
     1441 tcttctgtta gtgcatgcag aataagatct ttatcgttga actttgtagt gtgaataaca
     1501 atagcccgtg ttttagcaag catagctatt tttttatcaa tttataggtg tttgatgtat
     1561 cgtctcttgt tatttgcaaa aagtagatgc cttgttgtaa cccactcgta ttgatggtaa
     1621 gtggattttc gatgctactt attgtaaaga ggagtgttcc ttgtatcgaa aacagagaga
     1681 attgggctgt atttgtctct tttctaaata cttgaatttc agaatcgcat ggattgggaa
     1741 ataaaattgc attagcgtct tcatcgtgtg gataaaagat taaactttcg tttgcttctt
     1801 gaaatacagg ttcaatccat agcgaacttg ccattggttt gtaaggatgt tcggtaaacg
     1861 atttccatat tccattgtcc ttgtaataag cggtattgag tgcatctatt gctctgttat
     1921 ttaccatata cacagcaaac gaatctactg gttgataagt aatggaatag gaaacaaaaa
     1981 attcttcatt aactaaaaca ggatttggaa agcgtacata attttctcta tttaaaaatt
     2041 gagttttagt agtttgtgaa aatgtttgcg aagtagcttg atatgctagt gttttagggt
     2101 taaagagaac tgtcgttaat agtgtttcgg ggcttgatgt tccattataa acatgtacgt
     2161 ttacaggagt attcacattg tatttgccaa taaatggtac aatgtacacg ccatatagat
     2221 agttgttggt atctgttaca aatttctctg caaattctgt atattgcagt tcattatgtc
     2281 ccgacaaacg tccagtttta ccatttccta cagagacctt gcccattacg tctgcacttt
     2341 gtacgtttga ataacgtatg caaggggttg cttcgtatgg atttcttcca tccatataca
     2401 taacattggt attggtaggg tctaaccagc gttttaattg tttactttcg ctgctataat
     2461 aactccaagc tttgtttaaa cgaaagaaat aatcttctac gggactatta caggttgaat
     2521 tacctcctgt tagtccgcca ataatacggt agttgttgtc aaacaaaccc gaaccagaag
     2581 agcccggttc tgtcgttcct ttttcccatc tagttatttt ccagtgagca tctgctaatt
     2641 tggtttctag caaatcattg taggggtaag taactgcaag aggattgtca ttgtcggttg
     2701 acactcgttt aacatctcct tgtggatgct gtacgcatac cataggggct aaatcagagt
     2761 ttgctctatt ccatccagct aaataaggac ggtaatctgc tggtggcatt tggtttagtt
     2821 caaccagtgc aaaatcaata tcatacgctt tcgcttttaa tacacttcct cctaaagtaa
     2881 attcttgcga accacgaatg agtgagtcaa cttttggtac ctcataatta aaataaaaaa
     2941 tggttgtttg ttctaaatta gtcccttcgt aattatgtgc tgctgtaagc aaataaggtt
     3001 tcccctcgct attcttaatc attacgccag agcctaacca cgattcggaa ttgtcggtta
     3061 catacagcaa acatgtggct tgtttgatgt tgcttactcc ttcaacaaat gctgcatgcg
     3121 gactgcattt ctcagcacta ttaaacgatc cagtacttcg gttaataaaa tctattgtat
     3181 tgtgtgttac tctgccaata cgtagtttgc cgttaaaggt aactgtggca ggctgttggt
     3241 attctataat aatactatca gacggaaatg gtgcaatagg aaggctcttg ttactgttgt
     3301 tatttatact ggtaaatgca ccagctacat tggtttgaga tggattgtaa acaaaaatcg
     3361 aagccccttc gggtatttca aactcatcaa atactacgct aatagaggct gcatttggag
     3421 agtagaaccc aatacgccag attgttgtgc catcttcggt tgtaaattgt tgtccagcgt
     3481 ttaacggaga ataatctaca gcaaacgatt tgccaacaac aaagcgagat gttttttctt
     3541 ccttattgtc ttgttctcca ttatagggga ttttgtaaaa taacggctgc gtatttctct
     3601 ttccaataat ggtatgttgt tcgttcgaat acgtaaaagg tagtggcgaa aatcctccac
     3661 tttgtatctg agcgtagttt gtgccaatag aaaaaaacgc caatattacg actaataact
     3721 gtttcattgt caaaatttta atgcttgctt gcagattttt gcaatactaa aatggattca
     3781 tttcccatat taaacaacaa atcaagaata cttaaattag gaataaaacc aaatttattt
     3841 tcaaatactt gataatacga actaattaca aaatcgttat cttctatttc tttttttgca
     3901 gatagtgcat ctctgtaatc taacgcatca tcgtatgtgg tatatgcttc tgtcaattgc
     3961 actttacagt cgagttccaa taatgcaata accaacgatt gtaattgaaa ggtgtagtca
     4021 actaagaatt caaacttgtt agtataaaaa ggagcaaaat catctttgta atactcaaaa
     4081 aatggactcg aattataccc agcttctaaa gccctccagt gtatttgttg ccaatgc
//
LOCUS       DAOPUR010000074         4116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_21872_length_4116_cov_3.841911, whole genome shotgun sequence.
ACCESSION   DAOPUR010000074 DAOPUR010000000
VERSION     DAOPUR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21872_length_4116_cov_3.841911"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1594
                     /locus_tag="PKN90_01985"
     CDS             38..1594
                     /locus_tag="PKN90_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNZ85080.1"
                     /translation="MIENIDLTLVNWSRAQFAMTAFFHWLFIPLTIGMTYIIAFAQTL
                     HVRTNDPEWEKLAKFWAKLFGINFAIGVGTGIILGLEFGTNWSNYSWIGGDIFGAPLA
                     AEGIMAFFLESTFVAVMFFGWGRISKKFHLVACYLVAFGASLSALWILTANAWMNYPV
                     GMSFNPDIARHEMVDFWAILFSPFAVNKFLHTISSGFVIGSFFIIAVSSWYLLKGRSV
                     LFAKRSIFLASVFGLVGTGLVILTGDGSAYNVAQHQPAKLAAMEGLYDGKEGAGLVTV
                     GILTPGKKIGDNKDPFIFKQEIPYVLSFLGYRDINAFVPGINDLVYGYKSDKLEIVSA
                     SKKMEYGRVAMQALSLYKKTDNSEEKTMALQLFTEYQPFMGYAHITSPEKVIPNVPMI
                     FYSFHIMVMAGFFLIAVVAGLLFFSIKNKVENHKWLLRLGILGFPMAFIASMTGWIVA
                     EVGRQPWTIQDLLTTSVSTSNINSMAVILTFWMFFALLSVLVIAEVRIMLAAIKKGPE
                     STNTKGGENA"
     gene            1587..2723
                     /gene="cydB"
                     /locus_tag="PKN90_01990"
     CDS             1587..2723
                     /gene="cydB"
                     /locus_tag="PKN90_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNZ85081.1"
                     /translation="MLNSLSHFDLQQYWWLIMSLVGSILVFLLFVQGGQSLISTLGKT
                     ADERNMVVNSIGRKWEFTFTTLILFAAGMFAAFPLFYSTSFGGAYYAWKLFLIVFVIQ
                     AVSFEFRRKKGNLLGTKTYDTFLAINGYVAPFLLGAVVATFFTGSAFIRNEYNLSFWQ
                     TELYGIEILFEPTNLALGFAVLFLARILGAMYIINNTLENTIIERARKQVAINTVSFL
                     VFFLYFVYQLLVMDGYAVDPTSNQVYLEANKYLNNFLAMPISIVLFLVGVLLVIYGII
                     STWFMKSTKGIWFSGLGTVCTVLAIFFVAGFNNTSFYPSFIDLQSSLTIKNASSSHYT
                     LTAMTYISFLVPVVIAYIYFTWKAIDKHKITKEDIKEDAKHHVY"
     gene            complement(2921..3688)
                     /locus_tag="PKN90_01995"
     CDS             complement(2921..3688)
                     /locus_tag="PKN90_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNZ85082.1"
                     /translation="MNRMKKTMFIALLAMLLTSCAELLTIANQVIVTQPVAVSNTENI
                     AGLKSSLDVGIEKAVGMLSIENGFYNDAVLKVLLPPEAQPIVDNIKLIPGGQELVNKA
                     VLSLNRSAEDAVKEATPIFKKAILSMTIADATNILFGADNAATEYLRKTTYSDLSVAF
                     SPKVKESLGKPLVANVSTTESWKSLTSAYNQVANTPVGLIAGLKPVNVNLEQYVTEKA
                     LDALFVKVAEEEKAIRTDPVARINDILKRVFGQLDKK"
     gene            3873..>4116
                     /locus_tag="PKN90_02000"
     CDS             3873..>4116
                     /locus_tag="PKN90_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNZ85083.1"
                     /translation="MSNQRYMMRGVSASKEDVHKAIQHIDKGLYPKAFCKIIPDILGG
                     DPDFCNIMHADGAGTKSSLAYLYWKETGDISVWKGIA"
BASE COUNT         1211 a          744 c          783 g         1378 t
ORIGIN      
        1 actcatgatt taatacaacc attaaacacc atttcgtatg attgaaaaca ttgacttaac
       61 actggtaaac tggtcgcgag cccaatttgc catgacagcc ttttttcatt ggctttttat
      121 acctcttacc attggtatga cgtatatcat tgcctttgca caaaccctcc acgtaagaac
      181 caatgaccct gaatgggaaa aactcgcaaa attttgggca aaattatttg gtatcaactt
      241 tgctattggt gtgggcacag gcatcatttt gggtttagag tttggcacca actggtctaa
      301 ttattcgtgg attggaggcg atatttttgg tgcaccctta gcggctgaag gtattatggc
      361 attctttctc gaatctactt ttgttgctgt tatgtttttt ggttgggggc gtattagcaa
      421 aaagttccat ttagtagcct gttatctggt tgcctttggt gccagtttat ctgccctatg
      481 gatactaaca gcaaacgcat ggatgaatta cccagtgggc atgagcttta acccagatat
      541 cgctcgccac gaaatggtcg atttttgggc aattttattt tcaccttttg ctgtaaataa
      601 atttttacac accatctctt cgggttttgt aattgggtcg ttctttatta ttgctgtaag
      661 tagttggtat ttgctaaaag gacgcagcgt actgtttgca aaacgtagta tttttctagc
      721 cagcgtattt ggattagtgg gcacaggctt ggttatctta acaggcgatg gttcagcata
      781 taatgtagca caacatcaac ctgcaaaact tgccgccatg gaaggattgt atgatggcaa
      841 agaaggagca ggattggtaa ctgtaggaat acttacacct ggtaaaaaaa ttggagataa
      901 caaagacccg tttattttca aacaagaaat accttatgtc ctttcatttt tgggctatag
      961 agatatcaat gcatttgtgc ctggcatcaa tgatttggta tatggatata aatcagacaa
     1021 attagaaata gtatctgctt ctaagaaaat ggaatatgga agggtagcaa tgcaagccct
     1081 ctcgttgtat aaaaaaacgg ataatagcga agagaaaact atggctttgc aattatttac
     1141 cgaataccaa ccttttatgg ggtatgctca tattacaagt cctgaaaaag ttattccaaa
     1201 tgtaccaatg attttttata gtttccatat tatggtgatg gcgggctttt tcttgattgc
     1261 tgtagtagct ggattgttgt ttttcagcat caaaaataaa gtagaaaacc acaaatggtt
     1321 gttgcgattg ggtatccttg gtttcccaat ggcgtttatt gcctctatga caggatggat
     1381 agttgccgaa gtgggtcgcc aaccatggac aatacaagat ttgctgacca catctgtttc
     1441 tacatctaat atcaattcaa tggctgtaat tcttacgttt tggatgtttt ttgcactgct
     1501 ttctgtgctt gtaatagccg aagttcgcat catgttagcc gctatcaaaa aaggtcctga
     1561 atcaactaat acgaaaggag gggaaaatgc ttaattcatt atcacacttt gacttacaac
     1621 aatattggtg gcttattatg tcgcttgtag gttcgatact cgtttttctt ctttttgtac
     1681 aaggaggtca atcattgatt agtaccttag ggaaaacagc cgacgaacgc aatatggtgg
     1741 tcaactccat tggacgtaaa tgggaattta cgtttactac cttgattctt tttgcagcag
     1801 gtatgtttgc ggcatttcca ctgttctatt ccacttcgtt tggtggagct tattatgctt
     1861 ggaaactttt tttgattgta tttgtgatac aagccgtatc gtttgagttt agacgcaaaa
     1921 aaggcaattt gctaggaact aaaacatacg atacattttt agccatcaat gggtatgtag
     1981 ccccgttctt actaggtgct gtggtagcca cattctttac aggaagtgct tttattcgca
     2041 acgaatacaa tttatctttt tggcaaacag aattgtatgg cattgagata ttattcgagc
     2101 ctactaacct tgctttgggt tttgcagtgc tatttttagc acgcatattg ggagctatgt
     2161 atattatcaa caatacacta gaaaacacaa ttattgaacg agcacgaaaa caagtagcaa
     2221 ttaataccgt gtcctttttg gtctttttct tgtattttgt gtatcaactc ttagtaatgg
     2281 acggttatgc tgttgatcca acaagcaatc aggtatattt ggaagccaat aagtatttga
     2341 ataactttct ggctatgcca ataagtattg ttttgtttct tgtaggcgta cttttagtta
     2401 tttatggtat tattagcaca tggtttatga aaagtaccaa aggaatatgg ttttcgggat
     2461 tgggaactgt atgtaccgtt ttggctattt tctttgtagc tggctttaac aacacgagtt
     2521 tctatccgtc ttttattgat ttacagagtt cgttaacgat taaaaatgcc tcttccagtc
     2581 attacacatt aacagcaatg acctatatat cctttttagt gccggttgtt atagcataca
     2641 tttattttac ttggaaagct atagacaaac acaaaattac gaaagaagat ataaaagaag
     2701 acgcaaaaca tcatgtttac taaccctcat aaaacaacaa tactatgcaa catattgtat
     2761 ccattttatg gcttgtcagt tggcccatcc ttatacttgt aagttacaaa ctgattgttt
     2821 attttctaaa aatgatggaa aagagtaaag aaaaagagta aaacgaaaaa aggcagttca
     2881 caacgagctg cctttctttt tgtacacgat gattagttgc ttatttctta tctaactgac
     2941 caaacacacg ttttaaaata tcgttgatac gtgccacagg atctgtacga atggcttttt
     3001 cttcttcagc cacttttaca aacaaagcat ctaaggcttt ttctgtaacg tattgttcta
     3061 aatttacatt cacgggtttc aagcctgcaa tcaacccaac aggcgtattg gctacttgat
     3121 tatatgcact tgttaacgat ttccacgatt cggtagtaga tacattggct accaatggtt
     3181 tgcccaacga ctcttttacc tttggggaaa aagcgacact taaatcacta tatgttgttt
     3241 tacgcaggta ttcggtagca gcattatcgg ctccaaacaa aatattcgtt gcgtcggcta
     3301 tcgtcatact caagatggct ttcttaaaaa tgggcgttgc ttcttttact gcatcttctg
     3361 ccgaacgatt aagcgacaat acggctttgt ttaccaactc ttgaccacca ggtattaact
     3421 ttatattatc aacaatcggt tgagcttcgg ggggcaacaa aactttcaaa acagcatcgt
     3481 tataaaatcc attttcaata ctcaacatac ccacagcttt ttctatccct acatcaagcg
     3541 aactttttag acctgcaata ttttcagtat tacttactgc tactggttgt gttacaatta
     3601 cctgattagc aatggttaat aactcggcac acgaagttag caacatggct aacagggcaa
     3661 taaacatcgt ttttttcatt ctattcatag tcataatata ttttttgtgt tactaaacag
     3721 ggcaaagcta cacattgtac taaagaaaaa tagtgcaaaa aagaaaaaaa ctacttaatt
     3781 gtttataatt cttgattcag tatgcagtag ctaatctttt ctatttgtaa taatttgatt
     3841 acctttgccg aacaaaaaaa agcaagctta ccatgagcaa tcaacgttat atgatgcgag
     3901 gcgtatctgc ctctaaagaa gatgtgcata aggctattca gcatattgat aaaggattat
     3961 atcccaaagc tttttgcaaa attattccag atattttagg tggcgacccc gatttttgca
     4021 acattatgca tgctgatggg gctggcacaa aatcctcatt agcctatttg tattggaaag
     4081 aaacaggcga tatatctgta tggaaaggca tagcac
//
LOCUS       DAOPUR010000075         4081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_22126_length_4081_cov_3.511177, whole genome shotgun sequence.
ACCESSION   DAOPUR010000075 DAOPUR010000000
VERSION     DAOPUR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22126_length_4081_cov_3.511177"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1972
                     /gene="yidC"
                     /locus_tag="PKN90_02005"
     CDS             83..1972
                     /gene="yidC"
                     /locus_tag="PKN90_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNZ85084.1"
                     /translation="MDKNTLVGFLLIIGIFIGFFWISQPSAEQLEQRKRYNDSIILVQ
                     KEQSKLAEIEAIQQENVSTTVDNVPLDSVVDVNNAFGPFAKFTQGNNELLVLENKKLK
                     IGVDTKGGVIQTVELKEYLTHDKQTLQLMDGSSEKTNFTFVTANNRIVNSEDLYFQPI
                     WTNHGQSLIMRLAFDSISYIDFEYALKEDDYIVSFKITPHNVQHILSKRLTALDMEWA
                     THIPQQELGRKFENRYAAVYFKYFGDKDVEDLKADKNDERTIPNRLHWIASKGQFFSS
                     VLIADDYFMSSTLQSNLEPDTSRYLKSTKATMSVDFDTKGVKSTGFTYYFGPNKYKLF
                     KSYDKGKEESNKWDLDELIPLGWGIFGWVNKILVIPVFNFLEKFIDSYGIIILLLTLF
                     IKLVLLPLTYKSYLSMAKMKVLRPQIEEINAKIPAEKAMERQKATMALYKKVGVSPMG
                     GCLPMMLQMPILFALFTFFPASIELRQESFLWAKDLSTFDAIVSWDTYIPIISTYYGN
                     HISLFCVLMAITNIVYTKLNSDATGSTQQMPGMKYVMYLMPLMFLVFFNEYASGLSYY
                     YFISTLFTILQTYIFRLFIDDEKLLKKLNENKKKPSKKSRFAEKLEQLQKQQLELQKQ
                     QRNKK"
     gene            2089..2628
                     /locus_tag="PKN90_02010"
     CDS             2089..2628
                     /locus_tag="PKN90_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNZ85085.1"
                     /translation="MKKDKKLEETEESIQETSEQPEIAQNTQEEVSEIDSLKSQLEQV
                     NNSYLLLLADFDNYKKKTLKEKSDLLKYGGENVLSNLLPIIDDFERAMQSLEKEPDSA
                     IKQGIELIHGKFISFLQRNGVKEIDTKEKIFDTDYHEAITLFPAQQPEDKGKIVDCVQ
                     KGYQLHDKVIRYAKVVVAE"
     gene            2649..3767
                     /gene="dnaJ"
                     /locus_tag="PKN90_02015"
     CDS             2649..3767
                     /gene="dnaJ"
                     /locus_tag="PKN90_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNZ85086.1"
                     /translation="MAKRDYYEVLGISKNATEDEIKKAYRKKAIEFHPDKNPGNTEAE
                     EKFKEAAEAYDVLSDPQKKQRYDQFGHAGMGGAASSGAGFGGGMSMDDIFEHFGDIFG
                     GHFGFGSSSRQRTRVNKGTNLRVKVKLTLQEVAKGVEKKIKVKKYVTCSKCGGSGAED
                     ASSIVTCSSCKGTGSVTRVVNSMFGQMQTSSACPTCQGEGKIITKKCSKCNGEGVEIA
                     EEVISINIPAGVQEGMQLSMSGKGNAARRGGVNGDLLILIEEEPHPELIRDDNDLIYN
                     LMLSLPTAILGGTVEIPTIEGKAKIKIESGTQSGKILRLKGKGLPSVNSYGTGDLLVN
                     IGVYIPESLTKEEKKIFEGFEKSQSFSPSKSSKKNFFS"
     gene            3772..>4081
                     /locus_tag="PKN90_02020"
     CDS             3772..>4081
                     /locus_tag="PKN90_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNZ85087.1"
                     /translation="MNIAALVSGGVDSSVVVHRLKEMGYQPTIFYIQIGMEDETGYMG
                     CTAEEDIEIVSFIAKKYGLSMEVVSLQKEYWDQVVDYTIETVKKGITPNPDIMCNKLI
                     K"
BASE COUNT         1514 a          539 c          771 g         1257 t
ORIGIN      
        1 atacagtagt actgccgaaa ctccacaccc tttatttata aattttgtaa aagcagctat
       61 tagcaaataa taacttgaca atatggacaa aaatacacta gtaggttttc tacttattat
      121 cggaattttt attgggttct tttggatttc acaaccatca gcagaacaac tagaacaacg
      181 taaacgttat aatgattcaa tcatactagt acaaaaagaa caatcgaaat tagctgagat
      241 agaggctata caacaggaaa atgtaagtac tactgtagat aatgtgccct tagattcagt
      301 agtagatgta aacaatgcat ttggaccatt tgcaaaattc acacaaggta acaatgaatt
      361 gttagtatta gaaaataaaa agttaaaaat tggtgttgat acaaaaggag gtgtaataca
      421 aactgttgag ctgaaagagt atttaactca tgataaacaa acgctacaat taatggatgg
      481 gagtagtgag aaaacaaact ttacgtttgt taccgcaaat aatcgtattg taaactcaga
      541 agacttgtat tttcagccaa tatggactaa tcacggtcaa tccttgatta tgcgtttggc
      601 ttttgattca atttcttata ttgattttga atatgcatta aaagaagatg attatatagt
      661 ttctttcaaa ataacacctc ataatgtaca acatatacta tcaaaacgct taactgcgtt
      721 agatatggaa tgggctactc atatccctca acaagaatta ggtagaaagt ttgaaaatcg
      781 atatgctgct gtatatttta aatattttgg agataaagat gtagaagatt tgaaagcaga
      841 taaaaatgat gaaagaacaa ttcctaatcg tctacattgg attgcaagta aaggtcaatt
      901 tttttcctct gtgttgattg cagatgatta ttttatgtca tctacattac aatctaattt
      961 ggaacctgat acctctcggt atttaaagtc tactaaagca acaatgagtg tagattttga
     1021 tacaaagggg gttaagagta caggttttac ctattatttt ggtcctaata aatataaact
     1081 ttttaaatcc tatgataaag ggaaagaaga atcgaataaa tgggatttag atgaacttat
     1141 tcctttaggt tggggtatat ttggatgggt taataaaatt ttggttatac ctgtatttaa
     1201 ttttttggaa aaatttattg atagctatgg tatcattatc ttattactta ccctttttat
     1261 taaattagtg ttgttgcctc ttacctataa gtcatatttg tcaatggcaa agatgaaggt
     1321 acttcgccct caaattgaag aaattaatgc taagatacct gcagaaaaag caatggaaag
     1381 acaaaaagcc actatggctt tgtacaaaaa ggtaggagta agtcctatgg gaggatgttt
     1441 gccaatgatg ttacaaatgc ctattttatt tgcattgttt acttttttcc cagcatcaat
     1501 tgagttgagg caagaaagtt tcttatgggc aaaagatttg tctacatttg atgctattgt
     1561 gagttgggat acttatattc caatcatttc tacgtattat ggtaatcaca taagtctatt
     1621 ctgtgtatta atggctatta caaatattgt atatacaaaa ttgaatagtg atgctactgg
     1681 atctacacaa caaatgcctg gtatgaaata tgttatgtat ttaatgccac ttatgttttt
     1741 agttttcttt aatgaatatg catctggatt gagttattac tattttattt caaccttatt
     1801 tacaattcta caaacgtata tttttcgtct ttttatcgac gatgaaaaac ttctcaaaaa
     1861 actaaatgaa aataaaaaga aacctagtaa aaagtctcgt tttgctgaaa aattagaaca
     1921 attacaaaaa caacagttag aactccaaaa acagcaacgt aataaaaaat aagcataaag
     1981 gtgttctttt ttagaacacc tttttttaaa tactgccaaa atgacttgtt ttaaaagatt
     2041 ggcaagctta ttgcatgtag cttatcagat aattattagt aaagaaccat gaagaaagac
     2101 aaaaaactag aggaaacaga agaaagcata caagaaactt ctgaacaacc agaaatcgca
     2161 caaaatactc aagaagaagt tagtgaaatt gattcgttaa agagtcaatt agagcaagta
     2221 aataactcat acctactgtt gcttgctgat tttgataatt ataagaaaaa aacattaaaa
     2281 gaaaaaagcg atttattaaa atatggaggt gaaaatgtat taagcaattt acttcctatt
     2341 attgacgatt ttgaaagagc aatgcaaagt ttagaaaaag aacctgattc tgctataaaa
     2401 caaggtattg agctcattca tggaaagttt atttcctttt tgcaacgcaa tggagtaaaa
     2461 gaaatagaca ctaaagaaaa aatattcgat acagattatc acgaagccat tactctattt
     2521 ccagcacaac agccagaaga taaaggtaaa attgtggatt gtgttcaaaa gggatatcaa
     2581 ttgcatgata aagtgattcg ttatgctaaa gtagtcgttg cagaataaca atatagatta
     2641 acgtatatat ggcaaaaaga gattattatg aagttcttgg tataagcaaa aatgctactg
     2701 aagatgaaat taaaaaagct tatcgaaaaa aagctattga gtttcatcct gataaaaatc
     2761 ctggtaatac agaagctgaa gaaaaattta aagaagcagc agaagcatat gatgtgctaa
     2821 gtgatccgca aaagaaacaa cgttacgatc agtttgggca tgctggtatg gggggagcag
     2881 cctctagtgg agcaggattt ggcggaggta tgtctatgga cgatattttt gaacactttg
     2941 gagatatttt tggtggacat tttggatttg gtagcagtag tcgtcagcgt actagggtaa
     3001 ataaaggaac aaacttgcgt gttaaagtaa aattaacact tcaagaagta gccaaaggtg
     3061 ttgaaaaaaa gattaaggta aaaaaatacg ttacatgttc taaatgtggt gggagtggag
     3121 cagaggatgc ttcttctatt gttacatgtt catcatgcaa aggcacagga agtgtaacaa
     3181 gggttgtaaa ctcaatgttt ggacaaatgc aaacgagttc cgcgtgtcct acttgccaag
     3241 gtgaaggtaa aattattacc aagaaatgct ccaaatgtaa tggtgaagga gtagaaattg
     3301 ctgaagaagt aatttctatc aatattccag caggagtgca agaaggtatg cagctatcta
     3361 tgtcaggaaa aggaaatgct gcacgcagag gtggggtaaa tggtgattta ttgattttaa
     3421 ttgaagaaga acctcatcct gaattaatac gtgatgataa tgatttaatt tataatttaa
     3481 tgttatcatt gccaactgct attttgggtg ggactgtaga aatacctact attgaaggga
     3541 aagcaaaaat taaaattgag agtggaacac aatcgggtaa aatattacgt ttgaaaggca
     3601 aaggtttgcc tagcgtaaat tcttatggaa caggagattt attagtgaat ataggtgtgt
     3661 atataccaga atctttaaca aaggaagaaa agaaaatctt cgaaggattt gaaaaatctc
     3721 agtcattttc tccttctaaa tcatcgaaaa agaatttttt cagttaagta aatgaatata
     3781 gcagccttag tttcaggagg agtagatagc tcagtagtag tacatcgttt aaaagaaatg
     3841 ggctatcagc ctactatctt ttacatccaa ataggaatgg aggatgaaac aggctatatg
     3901 ggttgtacgg cagaagaaga tattgagata gtatcattta ttgccaaaaa atatggatta
     3961 tcgatggagg ttgtttcttt gcaaaaagaa tattgggatc aggtagtaga ttataccatc
     4021 gaaacagtaa aaaaaggaat tacaccaaat ccagatataa tgtgcaataa attgattaag
     4081 t
//
LOCUS       DAOPUR010000076         4080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_22140_length_4080_cov_3.410435, whole genome shotgun sequence.
ACCESSION   DAOPUR010000076 DAOPUR010000000
VERSION     DAOPUR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22140_length_4080_cov_3.410435"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="rnc"
                     /locus_tag="PKN90_02025"
     CDS             complement(<1..752)
                     /gene="rnc"
                     /locus_tag="PKN90_02025"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNZ85088.1"
                     /translation="MIKRIINRIRFISFTKNEPYFVLRNLLGFSPKENSYYQLALLHR
                     STGKRNINGVIIDNERLEFLGDAILSAIISDYLYQKFPLKKEGFLTNLRSKIVQREYL
                     DYLAVQMGLDKLMFSQNKHIVSKNAVHVYGNALEALIGAIYLDQGYKKCQQFLEKQVI
                     EKHMNVNEIVKKEYNFKSKLIEWAQKRKYSIIFVVTNSEINKEQNITTFYTSVLIENI
                     EVGTGCGLNKKESQQKAARDALHKIKKKETGIL"
     gene            complement(777..2036)
                     /gene="fabF"
                     /locus_tag="PKN90_02030"
     CDS             complement(777..2036)
                     /gene="fabF"
                     /locus_tag="PKN90_02030"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNZ85089.1"
                     /translation="MELKRVVVTGLGAVTPIGNDVPTFWNSLIQGVSGAKPITRFDAS
                     KFKTQFACEVKDFDPNQYFDRKDARKYDLFSMFGIVAAKEAVEDAGIDVEKVDLDQIG
                     VIFGTGIGGIKTFAEEVAGFVQNESIPRFNPFFIPKMIIDILPGHVSIMYGFRGINFS
                     TSSACASSTHALADAFNYIRLGKANIMVAGGSEAAITDVGVGGFNALHAISTRNDSPE
                     TASRPFSLSRDGFVMGEGAGCLIFEELEHALARGAKIYAEVKGAGMTADAHHMTAPHP
                     EGLGAINVMKSALKDADMQPDEIDYINVHGTSTPLGDIAETKAIKDVFGEHAYNLNIS
                     SSKSMTGHLLGATGAVEAIACVLSVQNDIVPPTINFTEGDEDPEIDYKLNFTFNKAQK
                     RPVNAALSNTFGFGGHNGSIIFKKFAK"
     gene            complement(2055..2288)
                     /locus_tag="PKN90_02035"
     CDS             complement(2055..2288)
                     /locus_tag="PKN90_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNZ85090.1"
                     /translation="MSEVAEKVKAIIVEKLGVEASEVVPAASFTNDLGADSLDTVEMI
                     MEFEKEFGMSIPDDQAQKIATVGDAISYIEANK"
     gene            2590..3150
                     /locus_tag="PKN90_02040"
     CDS             2590..3150
                     /locus_tag="PKN90_02040"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNZ85091.1"
                     /translation="MINVAIFASGSGSNAENIIRYFTGSQLVNFSCILSNKQEAFVHQ
                     RAKKLLIPSYTFTKQQFEDGTVLSFLMDEKINFIILAGFLLKVSDNIITQFPNKIVNI
                     HPALLPKFGGKGMYGDYVHQAVVDAHETQTGITIHFVNNKYDDGEIIFQATCDVLSTD
                     TPDDVAQKVHALEYEHFPHVLKMLWS"
     gene            3288..>4080
                     /locus_tag="PKN90_02045"
     CDS             3288..>4080
                     /locus_tag="PKN90_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ85092.1"
                     /translation="MKSFVHLHVHSYYSLLDGMSSISALVDKAQKDGMPAIALTDHGS
                     MFGIKEFYDYTKKKNKKVKEDIAILEKELLAATDDKQREVWKEKIAATTCKYVKPIIG
                     SEVYVAKRTRFDKTEEIDRSGNHLVLLAKNKKGYRNLCKLVSYGYIEGYYYRPRIDKE
                     LLEKYSEGLIVSSACLGGEIHRLVEKGNIEEAEKSILWFKRVFGDDYYLELQRHETDK
                     PGADTHVFEKQQRQNKVLIELARKTNTKFIATNDVHFVEEEHAEAH"
BASE COUNT         1346 a          663 c          643 g         1428 t
ORIGIN      
        1 agaatcccag tttctttttt ctttattttg tgtaaagcat ctcttgctgc cttttgttga
       61 gactcttttt tgtttaaacc acaacctgta ccaacttcta tattttcaat taatacacta
      121 gtataaaatg tggttatatt ttgttcttta tttatttcgc tatttgtaac tacgaatata
      181 atggaatatt ttcttttttg agcccattct attagttttg atttaaaatt gtactctttt
      241 tttacaattt cgttcacatt catatgtttc tcaataactt gtttctctaa aaattgttga
      301 cattttttat atccttgatc taggtaaatt gcaccaatta atgcttcaag agcatttcca
      361 tatacatgaa ctgcattttt agagacaata tgtttatttt gggaaaacat tagtttatct
      421 aaacccattt ggacagctag atagtctaaa tattctcttt gaacaatttt tgaacgtaga
      481 ttagttaaaa atccttcttt tttaagtgga aatttttgat acaaataatc agagattata
      541 gcagataaaa tagcatctcc taaaaattcc aatcgttcat tgtctataat aacgccgttt
      601 atattgcgtt ttcctgttga acgatgtaat agagctagtt ggtaataact attttctttg
      661 ggactaaatc caagtaggtt acgtaaaaca aaataaggtt cgttctttgt gaaagaaatg
      721 aaccttattc tattaataat tcgttttatc aatgctagta cacgttaaaa aagtgattac
      781 ttcgcaaatt ttttaaagat gatacttcca ttatgcccac caaatccaaa agtattcgat
      841 aaagctgcat ttacaggtct tttttgggct ttattaaaag taaagttcaa tttataatct
      901 atttcaggat cttcatctcc ttctgtaaaa ttgatggtag gaggaactat gtcattttgt
      961 acactaagta cgcatgcgat agcttctaca gctccggtag ctcctaataa atgtcctgtc
     1021 attgattttg acgaactaat attcaaattg tatgcatgtt cgccaaatac atctttaatt
     1081 gcttttgttt cagcaatatc acctaatggg gtagaggtac catgaacatt gatataatca
     1141 atttcatctg gttgcatgtc tgcatctttt agtgcagatt tcattacatt tatagctccc
     1201 agtccttctg gatgtggagc tgtcatatga tgagcatctg ctgtcatacc agcacccttt
     1261 acttctgcat atattttagc acctcgtgct aaagcatgtt ctaactcttc aaaaatcaaa
     1321 caaccggctc cttcacccat cacaaaaccg tctctagata aactaaaagg acgtgaagct
     1381 gtttctggac tatcattacg tgtggaaatg gcatgtaaag cattaaaacc tcctacacca
     1441 acatcagtta tggcagcttc tgagccacct gcaaccataa tgttagcctt gcctaaacgt
     1501 atataattaa aggcatcagc caaagcatgt gtacttgaag cacaagccga agatgtagaa
     1561 aaattaatac cacgaaatcc atacatgata gatacatgac caggtaaaat atcaataatc
     1621 atttttggaa taaaaaatgg attgaaacgt ggtatagact cattttgaac aaaaccagcc
     1681 acttcttcgg caaatgtttt tataccacca attcctgttc caaaaataac gccaatttga
     1741 tctaagtcta ctttctcaac atcaatacca gcatcttcta ccgcttcttt tgctgctacg
     1801 atgccaaaca ttgagaataa gtcatattta cgggcgtctt ttctgtcaaa atattgattt
     1861 ggatcaaaat ctttaacttc acacgcaaat tgagttttga actttgatgc atcaaaacgc
     1921 gtaataggct tagctccact tacaccttgt attaacgaat tccaaaatgt aggaacatcg
     1981 ttgccaatag gagtaactgc acctaaacct gttacaacaa ctctttttaa ctccatgaat
     2041 aaagttaact taaattattt gtttgcttca atgtaagaga tagcatcacc cactgtagca
     2101 attttttgag cttggtcatc tggaatagac ataccaaatt ctttttcaaa ttccatgatc
     2161 atttctactg tatcaagaga atctgcacca agatcgtttg tgaagctagc tgctggaact
     2221 acttctgatg cttcaacacc taatttctct acaatgatag cttttacttt ttctgcaact
     2281 tcagacataa cttttaattt ttagttaata aataaattaa atttgtgaaa ttttagcgat
     2341 gcaaatgtac actttttttt gcgaatacaa gacatttctt caaaaaaatg tgcacacgta
     2401 tttaattttt gtgcactata attcttattt atttattatt aaatatttgt atttcaatta
     2461 gatatattta aaataaaata agaattgttg ttaataaatt ttcttttgtg attaatattg
     2521 tgcatctaaa ttcttaattg tacctttgtg ttttcttttg aaagtataaa acataagtgc
     2581 cttttttata tgataaatgt tgccattttt gcatcaggtt caggaagtaa tgctgaaaat
     2641 ataattcgtt attttacagg tagtcagtta gttaattttt catgtattct ttcgaataaa
     2701 caagaagcat ttgtgcatca acgtgccaaa aaacttttaa ttccttccta tacatttacc
     2761 aaacaacagt tcgaagatgg aactgtgttg tcttttctta tggatgaaaa gataaatttt
     2821 attatcctag caggtttttt attgaaagta tcagataata ttattacaca atttccaaat
     2881 aaaattgtaa atattcaccc tgctttgtta cctaaatttg gaggcaaggg gatgtatgga
     2941 gattatgtac atcaagcagt ggtagatgct catgaaacac aaactggaat tacgatacat
     3001 tttgtaaata ataagtacga tgatggtgaa ataatttttc aagctacgtg tgacgtattg
     3061 tcaacagata ctccagatga tgttgctcaa aaggtacatg ctttagaata tgaacatttt
     3121 ccgcatgtat taaaaatgtt atggtcataa attttcacta tatcttttga cacttaaaca
     3181 gttatgggta gacctagctt tttgtttaaa actttataga attttatttt ttctacttta
     3241 agagaatcac taactttgga tttctgaaat aatttacctt tttatatatg aaatcctttg
     3301 tacatttaca cgttcactct tattattctt tattagatgg tatgtcttct attagtgcct
     3361 tggttgataa ggctcaaaaa gatggcatgc ctgctattgc tcttacagac cacggttcaa
     3421 tgtttggcat aaaagagttt tatgattata ccaaaaagaa aaataaaaaa gtaaaggaag
     3481 atattgctat attagaaaag gaattactag ctgcaacaga cgacaaacaa cgagaagtgt
     3541 ggaaagaaaa aattgcagca actacttgta agtatgtcaa accaattata ggaagcgaag
     3601 tgtatgtagc taagcgtact cggtttgata aaacagaaga aatagacaga agtggaaatc
     3661 atcttgtatt gttagcaaaa aataaaaagg gctatcgaaa tctatgtaaa ttagtttcgt
     3721 atggatatat cgagggttat tattatcgtc ctcgcattga caaagaactg ctagaaaaat
     3781 acagcgaagg cttgattgtg tcttcggctt gtttgggggg tgaaattcac cgtcttgtag
     3841 aaaaagggaa tatagaggaa gcagaaaaaa gtattttgtg gtttaaacgt gtttttggtg
     3901 atgattatta tttggaattg caacgacatg aaacggacaa accaggagca gatacacatg
     3961 tatttgaaaa gcaacaaaga cagaataaag tattaataga acttgcacgt aaaacgaata
     4021 cgaaatttat tgcaaccaat gatgttcatt ttgtagaaga agaacatgca gaggctcatg
//
LOCUS       DAOPUR010000077         4078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_22155_length_4078_cov_3.442704, whole genome shotgun sequence.
ACCESSION   DAOPUR010000077 DAOPUR010000000
VERSION     DAOPUR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22155_length_4078_cov_3.442704"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PKN90_02050"
     CDS             complement(<1..417)
                     /locus_tag="PKN90_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85093.1"
                     /translation="MKQLDNANERFAQLLKNYESNLTGGYFDAEEMEQLADFYLRRMR
                     VKDAKRAIEFGLKLHPNNIGLETSKVRILIELNDLELALQHTNSLVALYPSDIELHLL
                     QTEILLKTKQEVQAEKNCKELINTYAQEDETIYLDIA"
     gene            complement(513..1103)
                     /locus_tag="PKN90_02055"
     CDS             complement(513..1103)
                     /locus_tag="PKN90_02055"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNZ85094.1"
                     /translation="MIYLETSRQSEKRRGSIEVICGSMFSGKTEELIRRLKRAKIAQL
                     HVEIFKPAIDVRYSETEVVSHDKNSILSTVVDSSQNILLLSNDVDVIGIDEAQFFDMG
                     LIDVCRQLANQGMRVIIAGLDMDFKGNPFGPMPALCAIAEHVTKVRAICVKCGHLALY
                     SYRLTNSDKQVLLGETADYQPLCRECYHKSTTKLKE"
     gene            1168..1896
                     /locus_tag="PKN90_02060"
     CDS             1168..1896
                     /locus_tag="PKN90_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85095.1"
                     /translation="MEKNILIDQMLKDIKELELIVNGVRTMNTIHPIMQQLVARKFKN
                     LEENFSLVLQDSVPTDKGAPKFTDVNDEEDSITSTDDATTTILLDEDIEEIEFVDTRK
                     KEQPVSQPTVKSSTSPTIPPSQNEPRTYTQQKSSIQPETTIHKETPKPASVILATPKT
                     SAVDARLVTDLRRAIGINDRFRFKRELFGGDEQKMMETIDILNQCSSLQEVEEYIDAH
                     FDWQKEDSTYVYFSEILHKRFAHI"
     gene            1906..2619
                     /gene="rsmI"
                     /locus_tag="PKN90_02065"
     CDS             1906..2619
                     /gene="rsmI"
                     /locus_tag="PKN90_02065"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNZ85096.1"
                     /translation="MKLFVVPTPVGNLDDMTFRAIEVLKKVDFILAEDTRTSAVLLKH
                     FDIKNTLIAHHKFNEHKGLQPIIERLLAGQTGAVISDAGTPGISDPGFLIVRACIEHN
                     IPVECLPGATAFVPALVNAGLPMDRFCFEGFLPQKKGRQTRLQQLKFETRTIILYESP
                     YRVVKTLTQLAEILGQNRKASVSREISKLYNETINGTLADIIAHFVQKQPKGEFVLVI
                     AGANVKHAELDDSSSEEDE"
     gene            2722..3573
                     /locus_tag="PKN90_02070"
     CDS             2722..3573
                     /locus_tag="PKN90_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85097.1"
                     /translation="MKKLLLTSAILAILFSSCGYESKEFKALKAKNDSILAVQEQQIA
                     EMNEYMALVQEIDNGFSAIKQSQDMLSISSSYEGQPNESLRTRFTNDLNTINQILADN
                     KSKIADLEKKVESGKFQSSELRRTVNRLTETLNQKNQELEALSAVLAKKDITIDSLVV
                     ETQTLLRKTADLEMEKNVNLQEIAKQDADLNRVYYKLATRKEIKDSNIDVDKMKSTIR
                     NGMFTAADKRELDAVEINSKRAKVLTKHPVSSYELRQKADKTYTLIIKNHTDFWSMSK
                     YLLVRID"
     gene            3652..>4078
                     /locus_tag="PKN90_02075"
     CDS             3652..>4078
                     /locus_tag="PKN90_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ85098.1"
                     /translation="MKYKHLFFDLDNTLWDFDKNSQVSLHELFDAYQLIHYYPRFEDF
                     FEIFKKQNEVLWEMYGKGQIDKNFLHYERFRFPFENTPFEAQLRYKELADEYLLVVKT
                     KTHLKPFAKEILEYLKDKNYMMYIVSNGFTEVQYHKLENA"
BASE COUNT         1408 a          670 c          731 g         1269 t
ORIGIN      
        1 agcaatatcc aaatagattg tttcatcttc ttgtgcataa gtattaatta gctctttgca
       61 gtttttttct gcttgtactt cttgcttggt tttgagcaat atttcggttt gtagtagatg
      121 taattctata tcagagggat ataaagctac taatgaattg gtatgttgca aagctagctc
      181 taggtcgttc aattctatta agatacgtac tttagatgtt tctaacccaa tattgtttgg
      241 gtggagtttg agcccaaatt caatagctcg tttagcatcc tttacacgca ttcttcgcaa
      301 atagaaatca gctaattgct ccatttcttc tgcatcaaaa tagcctccag taagattgga
      361 ttcataattt ttaagaagtt gagcgaaccg ttcattagcg ttatctaact gcttcatggt
      421 attccttttt ctgcaaaaat acaagtaaat tggctgataa cgaaacttat acattaaaag
      481 ttattaacaa caaaaaatgt gttgattgta atttattctt tcagttttgt tgtagatttg
      541 tgatagcact ctctacataa tggttgatag tcggctgttt ctccgagcaa cacttgtttg
      601 tccgaatttg ttaaacgata cgaataaagg gctaaatgac cacatttaac acaaatagct
      661 cttactttgg taacatgttc agcaatagca cataaagcag gcattggacc aaacggatta
      721 cccttaaagt ccatatccaa acccgctatg attacacgca ttccttgatt ggctaattgt
      781 ctacacacat ctattaatcc catatcaaaa aattgagcct catcaatacc tattacatca
      841 acgtcatttg aaagtagtaa tatattttgt gaagagtcaa ccaccgttga taatattgag
      901 tttttatcgt gcgatacaac ctctgtctcc gaataacgca catctatggc aggtttaaat
      961 atctctacat gtaattgtgc aatttttgcc cgttttagac gacggataag ttcttcggtt
     1021 tttccagaga acatagatcc gcaaataact tcgattgaac ctcttcgttt ttctgattga
     1081 cgtgatgttt ctaaatatat catttgttgc ttttgagttt aattactact tttgcaaaaa
     1141 taattaattt cttcagaaaa aaggctcatg gaaaaaaata ttttaattga ccaaatgctc
     1201 aaagatatca aagagcttga attaattgtg aatggtgtgc gtacaatgaa tacgattcat
     1261 ccaataatgc aacaattagt agctagaaaa tttaaaaatt tggaagaaaa cttttcgttg
     1321 gtattgcaag atagtgtgcc tacggataag ggtgctccaa agtttactga tgttaacgat
     1381 gaagaagata gtattaccag tacagacgat gctaccacaa ctattttgct ggatgaggat
     1441 attgaagaaa ttgagtttgt ggatacacgc aaaaaagagc aaccagtttc ccaaccaaca
     1501 gtaaagtcaa gcacatcacc tactattcca ccttcacaaa atgaaccgcg tacgtatact
     1561 caacaaaaat ctagtattca accagaaacg accattcata aggaaacccc aaagcctgct
     1621 tctgtcattt tagcaacacc caaaacaagt gctgtggatg ctcgacttgt aaccgatttg
     1681 cgtagagcga ttggtattaa tgataggttt cgttttaaac gcgaattatt tggtggagat
     1741 gaacaaaaaa tgatggagac aatagatata ttaaatcaat gttcgtcatt gcaagaggta
     1801 gaagagtata ttgatgccca ttttgattgg caaaaagagg atagtacata cgtctatttt
     1861 tcagaaatat tacacaaacg ttttgctcat atataaaaaa attgtatgaa actatttgta
     1921 gtgcccacgc cagtaggaaa tttggacgat atgacatttc gggctataga ggtgctcaaa
     1981 aaagtcgatt ttattttagc cgaagacaca cgaacaagtg ctgttttact caagcatttt
     2041 gatattaaaa atacccttat tgctcatcat aaatttaacg agcacaaagg attacaaccc
     2101 attatagaac gcttgttagc aggacaaaca ggggctgtta tttctgatgc tgggacacct
     2161 ggtatttctg atcctggttt tttgatagta cgtgcctgta tcgaacacaa tattccagta
     2221 gaatgtttgc caggagcaac agcttttgtt cctgcattag ttaatgctgg tttgcccatg
     2281 gaccgttttt gttttgaagg ctttttaccc caaaaaaaag gcagacaaac acgtttgcaa
     2341 caattaaaat ttgaaacgcg taccattatt ttgtacgaat ctccgtatcg ggtagtgaaa
     2401 acattaacac agttggcaga aatattggga caaaatcgta aagcctctgt atcgcgcgaa
     2461 atatcaaaac tgtacaacga aacaattaat ggcaccttgg cggatatcat agctcatttt
     2521 gtgcaaaaac aacccaaagg tgaatttgtg ctagtaattg caggtgcaaa tgtaaaacat
     2581 gcggaattgg atgactcttc atcagaagag gatgaatgaa attttatcca aaattttatc
     2641 taaaaaaata attgatttgt aattgattat gtatctttgt aaccgaaaaa cacaagtaac
     2701 acttttttaa aacattattt tatgaaaaaa ttactattaa catctgcaat tcttgctatt
     2761 ctgttttcat catgtgggta tgaaagcaaa gaattcaagg ctcttaaagc aaagaatgat
     2821 tctattttgg cagtacaaga acaacaaatt gccgaaatga atgaatatat ggctcttgtt
     2881 caagaaattg ataatggctt ttcggctatc aaacaatcgc aagatatgtt gagtattagt
     2941 tcttcttacg aaggtcaacc taacgaatca ttgcgtacac gttttacaaa tgatttaaac
     3001 acgattaacc aaattttagc cgataacaag tcaaaaattg ccgacttaga gaaaaaagta
     3061 gaaagcggaa agtttcaatc gtcagaattg cgtcgtaccg taaatagact aaccgaaaca
     3121 ttaaaccaaa aaaatcaaga actagaagca ttaagtgctg tgttggctaa aaaagacata
     3181 acgattgata gtttagtggt agaaacacaa acattgttac gcaaaacagc tgacttggaa
     3241 atggaaaaaa atgtaaattt gcaagaaatt gccaaacaag atgctgattt aaatcgtgtg
     3301 tactataaac tggctacacg caaagaaatt aaagatagca atattgatgt agataaaatg
     3361 aaatcaacta tccgcaatgg aatgtttacc gctgccgata aacgtgaatt ggatgctgtt
     3421 gaaattaatt cgaaacgtgc taaagtatta actaagcacc cagttagttc atacgagtta
     3481 cgtcaaaaag ctgataaaac atataccttg attattaaaa accatactga tttttggagt
     3541 atgtctaaat atttattggt acgtattgat tagtaaaaag ttaaataaag gtggtaaaag
     3601 tacgcaagaa tttattttct gcgtactttt gtttttatat cgtggtaaga aatgaaatat
     3661 aaacatctat tttttgattt agataatacc ttgtgggatt ttgataaaaa ttcgcaagta
     3721 agcttgcacg agcttttcga tgcataccag ctaatacatt actacccaag gtttgaggat
     3781 ttttttgaga tatttaagaa acaaaacgaa gtgttatggg aaatgtatgg gaaagggcag
     3841 atagataaaa attttttgca ctacgagagg tttcgttttc cttttgaaaa tacacccttc
     3901 gaggcacaat tgcgttataa agagttagcc gatgagtatt tgttggtagt gaaaacaaaa
     3961 acgcatctta aaccttttgc caaagaaata ttggaatacc tcaaggataa aaattacatg
     4021 atgtacattg tatccaacgg atttacagag gtgcaatacc ataaactgga aaatgcag
//
LOCUS       DAOPUR010000078         4061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_22267_length_4061_cov_3.396655, whole genome shotgun sequence.
ACCESSION   DAOPUR010000078 DAOPUR010000000
VERSION     DAOPUR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22267_length_4061_cov_3.396655"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PKN90_02080"
     CDS             <1..587
                     /locus_tag="PKN90_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ85099.1"
                     /translation="FASQSTMYPLWIADNTLTFRGTKLAGNAVQNGSNFTLPALSWGY
                     ADNQPNNSTNDKTSFNIDWAVDASGNPVQLTHIDFIKIYTAMVQEAGWLGETSTEIAG
                     VEDLHPNASLPTVIHSQENKQMYIQNPFNETLIVKANENCEMILYTISGNLIGKYMIT
                     PGENTITTSQLPTGNYIVHIINTKQNHIYKLIKN"
     gene            591..1625
                     /locus_tag="PKN90_02085"
     CDS             591..1625
                     /locus_tag="PKN90_02085"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HNZ85100.1"
                     /translation="MKTTLLTLISLLLLASNTFATNIVLNLSNPTTPANINYDTEKKY
                     WTDTYSESSDYTWLQFQNFKLAHLIGQNSYGGTYWDGFTLSTSGDNTDYSSTGWTGNQ
                     WGCMAGGGIKTDQSGAIVTTNGVVDTEQGIPYLVANYSAYANNGYSNEIKFADGQKYE
                     AVEVYVTNHPWPYYENMNGGMFTRILNQEGDYVKLIANGLDENGNSTGSASFYLAKYE
                     NGSLTQVTNWEKFDLSALGTIASLYFTIESTDNGDWGMNTAAYFCMDKLTVKEYTTSI
                     PQLATENAAYRVNNILYNIPENATITVYNLQGQLVYSTMATSNTVSLPMVNNHLIIKI
                     VNENNTQIIR"
     gene            1803..>4061
                     /locus_tag="PKN90_02090"
     CDS             1803..>4061
                     /locus_tag="PKN90_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ85101.1"
                     /translation="MNIYTLKTILIATLLAAIGFSGFAQAEQGGALRGRVYNPDNNQP
                     VDFAYVIIDGTTIGTITDLDGNFAIFGLKPGYVRVAVNSLGFDPVVTKEILITNANTP
                     YIEIEMKPSNIQLDDVVVEASRFRQREESPLSLRSIGIKEIEKSPGGNRDISKVIQSL
                     PGVGATPANRNDIIVRGGGANENRFFLDGVEIPNINHFATQGSSGGPAGIINVDFVRE
                     LDFFAGAFPANRGNALSSVLDFRLIDGNKDRLKTRATVGASDLALTLDGPIGDKSSFV
                     FSVRRSYLEFLFDLIGLPFLPTYNDIQFKHKIRFNQNNELSFIGLGAFDVNRLNLSAN
                     ETESQRFILKYLPTNDQWTYTVGAVYRNFFKNGNQTLVLSRNHLNNVSQKYQDNIEAP
                     TNLNFDYASNEIETKIRYEYVTRQGAWKFLYGAGAEYNQYTNYTYNKLFFNAQVVEFD
                     YETELDFYSYSAFTQISRNLLAERLLVSAGARIDGNTYSEQMSNPLEQFSPRISASYA
                     LTNKWSLNANTGRYYQRPAYTTMGYRDIAGNLINKDNRLRYKYTDHFVVGVENKPNPN
                     SRFTVEGFYKLYNDYPFSISDSVAISSKSADFDVFGAEAVLSIGEGRTYGVEILYRNT
                     DLWGSNITTSFTLFRSEFKDSKEVYIPTAWDNRILFNLTATRPLPKNWDIGFKWRYVG
                     GAPYTPVDLERSSLRQAYDVTGRTYLDYDRFNSERLAPSHQLDVRVDKAFFFKKWSLM
                     LYLDIQNIYNFKS"
BASE COUNT         1350 a          814 c          748 g         1149 t
ORIGIN      
        1 cttttgcctc acaaagtacc atgtacccat tgtggatagc tgacaatacg ctcacattta
       61 ggggaaccaa actagcgggc aatgccgtac agaatggctc caattttacc ttgcctgcct
      121 tatcgtgggg ttatgcagat aaccaaccga ataatagcac caatgataaa actagtttta
      181 atattgactg ggcagtagat gcatcaggca atccagttca acttacccat attgatttta
      241 tcaaaatcta tacagcaatg gtacaagaag ctggttggct tggcgaaaca tcaaccgaaa
      301 tagcaggcgt ggaagatttg caccccaatg cctcattgcc aactgtaata cattcacaag
      361 aaaataagca aatgtatatt caaaatcctt ttaatgaaac attgattgtg aaagccaatg
      421 aaaattgtga aatgatttta tataccatct ctggtaattt aatcggaaaa tacatgatta
      481 ctccaggcga aaatacaatt actacatcac aacttcctac tgggaactat attgtacata
      541 ttatcaacac aaaacaaaac catatttaca aactaataaa aaattaaacc atgaaaacaa
      601 ctttactgac actgatttct ttgcttttat tggctagcaa tacttttgcc accaatatcg
      661 tgttgaacct aagcaaccca acaacccctg cgaacattaa ctacgacaca gaaaaaaaat
      721 actggacaga tacgtacagc gaatcatctg actatacatg gttgcagttt caaaatttta
      781 agttagcaca cttaatcggc caaaattctt atggcggaac atattgggac ggttttactc
      841 tctcaacaag tggtgataat acagactata gctccactgg gtggacagga aatcaatggg
      901 gctgtatggc aggcggagga attaaaaccg accaatcggg agctattgta actacaaatg
      961 gtgtagttga cacagaacaa ggaataccat atcttgtagc gaactactct gcgtatgcta
     1021 ataatggcta tagtaacgaa ataaaatttg ccgatggaca aaaatacgaa gctgtagaag
     1081 tatatgttac aaaccaccct tggccctatt acgaaaatat gaatggaggt atgtttacac
     1141 gtatcctcaa ccaagaaggt gattatgtaa aactgattgc caatggctta gacgaaaatg
     1201 gaaatagcac aggttcggca agtttctact tagctaaata tgaaaatggc tcgttaacgc
     1261 aagttaccaa ttgggaaaaa ttcgatttat ctgcacttgg taccattgca tctctgtatt
     1321 tcacaataga atcaactgac aatggcgatt ggggcatgaa tacagctgca tatttttgca
     1381 tggataaatt aactgtaaaa gaatatacta caagcatacc acaattagca accgaaaatg
     1441 ctgcctatag agtcaacaat atcctataca acataccaga aaacgcaaca ataacggtat
     1501 ataacttaca aggacaactt gtttattcca ctatggctac atcaaatact gtaagcttac
     1561 ccatggttaa caatcactta attattaaaa ttgtaaacga aaacaataca caaattatcc
     1621 gataatacct tactgtgtct aaaacctgtt acgtttgatt ctttctgcca taattgcatc
     1681 aaattacgta acaggttttt cttatttttt aaatttattt tcttatggaa atatttttta
     1741 tatcaaattt attttctact tttgtgttaa gtaaaaaaaa gaatcactca gcacaattta
     1801 caatgaatat atatacatta aaaaccatcc ttattgccac actactagca gcaataggat
     1861 tttcgggttt tgcacaagca gaacaaggag gtgcattacg aggtagggtt tacaatccag
     1921 acaacaacca accggttgat tttgcgtatg tcattatcga tggcactacc attggtacta
     1981 ttacggactt agacggtaat tttgctatct ttggtttaaa acctggttat gtacgggtag
     2041 cagttaattc gcttggattc gaccctgtgg taacgaaaga aattctaatt accaacgcta
     2101 acacgccgta tatcgaaatt gaaatgaaac catcaaacat acagttagat gatgtggtgg
     2161 tagaagcatc tcgttttagg caacgcgaag aaagtccact cagtttacgc tccatcggca
     2221 tcaaagaaat agaaaaaagc ccaggcggta atcgcgatat ttctaaagta attcaatctt
     2281 taccaggagt aggtgctacc ccagccaacc gcaacgatat tattgtgcgt ggtggtggtg
     2341 ccaacgaaaa ccgctttttt cttgatggcg tagaaatacc caacatcaac cactttgcta
     2401 cacaaggttc gagtggcgga ccggcaggga ttatcaacgt agattttgta cgcgagctcg
     2461 acttttttgc gggagcattt cctgccaaca ggggcaatgc attgagttct gtgctcgatt
     2521 ttcggttgat agatggcaat aaagaccgtt taaaaacacg tgccacagta ggtgctagcg
     2581 atttggcact tacgcttgat ggtccgatag gcgataaaag ttcgttcgta ttttcggtaa
     2641 gacgctcgta tttagaattt cttttcgacc tgattggact gccctttttg cctacttaca
     2701 acgatatcca atttaagcat aaaattcgtt ttaaccaaaa caatgaacta agctttattg
     2761 gcttaggtgc ttttgatgta aacaggttaa acctcagtgc caacgaaaca gaaagccaac
     2821 gttttatact taaatacctg cctaccaacg accaatggac atataccgta ggggctgtgt
     2881 accgcaattt ctttaaaaac ggtaaccaaa ccttggtttt gagtcgcaac cacctcaaca
     2941 atgtatcgca aaaataccaa gacaacatcg aagcacctac taacctcaat tttgactatg
     3001 cctcgaacga gatagaaaca aaaattaggt acgaatatgt aacacgtcaa ggtgcgtgga
     3061 aatttttgta tggtgctggt gctgaataca atcaatatac caattatacc tacaacaaat
     3121 tatttttcaa tgcacaagta gtagagtttg attacgaaac tgagctcgac ttctattcct
     3181 attcggcatt tacacaaatc agccgcaatt tactcgctga gcgattactc gtatcagcag
     3241 gagcccgcat agatggcaat acctacagcg aacaaatgtc aaacccattg gaacaattct
     3301 ctccacgtat ttcggctagt tatgcattaa caaataaatg gtcgttaaat gccaatacag
     3361 gacgctacta ccaacgtcca gcttatacca ccatgggata cagagatata gcgggtaatc
     3421 taattaataa agataaccgt cttcgctaca aatatactga ccattttgta gtgggtgtag
     3481 aaaataaacc aaacccaaat agccgattta cagtagaagg tttttataaa ctttacaatg
     3541 attatccttt ctccatcagc gattcagtag ctatctcatc caaaagtgcc gattttgatg
     3601 tatttggagc agaagcggtt ttgtctattg gcgaaggtcg cacctatggg gtagaaatac
     3661 tgtatcgcaa tacggattta tggggaagca atattaccac cagttttacc ttattccgca
     3721 gcgaatttaa agactctaaa gaagtgtata tccccacagc atgggacaat cgtatattgt
     3781 tcaatcttac cgccaccaga ccattgccca aaaactggga tatagggttt aaatggcgct
     3841 atgtgggagg tgctccttat actcctgtag acttagaacg ctctagtttg cgacaagcat
     3901 acgatgtaac aggcagaacg tatttggatt acgatagatt taattccgaa cgattagctc
     3961 cttcgcatca attggatgta cgtgttgata aagcgttctt tttcaagaaa tggtcgctga
     4021 tgctttattt ggatatacaa aatatctata atttcaaaag c
//
LOCUS       DAOPUR010000079         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_22296_length_4057_cov_3.561469, whole genome shotgun sequence.
ACCESSION   DAOPUR010000079 DAOPUR010000000
VERSION     DAOPUR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22296_length_4057_cov_3.561469"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKN90_02095"
     CDS             complement(<1..262)
                     /locus_tag="PKN90_02095"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNZ85102.1"
                     /translation="MLLISNGRIVNEGKIYKASILIKDGKIDAIYKDEVPKSIVEKVH
                     TIDADDCWVLPGVIDSHVHFRDPGLTHTGEFPTESRAAAAGGV"
     gene            complement(296..1033)
                     /locus_tag="PKN90_02100"
     CDS             complement(296..1033)
                     /locus_tag="PKN90_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNZ85103.1"
                     /translation="MSDSLVLIPTYNEKENIENIIRAVFALPKSFHILVIDDNSPDGT
                     ADIVKRLQVEFSTQLHLLERKAKNGLGRAYIAGFNWALASAYEYIYEMDADFSHNPND
                     LLRLYEACSQGGADVAIGSRYVTGVNVVNWPMSRVLMSYFASKYVRFVTGLAIADTTA
                     GFKCYKRAVLETIELDKIKFVGYAFQIEMKFTAFKCGFNIKEVSVIFINRELGTSKMS
                     GKIFREAVIGVLQLRWESFFRKFPQKK"
     gene            1168..>4057
                     /gene="mfd"
                     /locus_tag="PKN90_02105"
     CDS             1168..>4057
                     /gene="mfd"
                     /locus_tag="PKN90_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNZ85104.1"
                     /translation="MDIKEFSQSLTSHRVVNGINQWVESSHKNVCIQGFCGSSCALFF
                     ASSILAKTNTVYILNDAEEAAYFYHDLLQLLSPDDVYYFPSSYKKRVREGDQKDAANQ
                     VLRTEALNDISQKQSWILVTYPEAIAERVIPQQDLSKETLHLAVGEKLDSDFVISLLV
                     EYGFERVDFVYEPGQFAVRGSIIDVFSFSHDVPFRIDFFGDTIDTIRTFDIESQLSIE
                     KKTHISIVSNSVNIEENALVPLFKFFTNKVVYTIKNTPYVVDLIDKINQENDETRFIL
                     STEWIEQWQGNKVIEFGNSPFFSSPHIITLNQNIQPIFHKNFDLLADSLTEYIAQQYQ
                     IYVLSDNPKQTERLKDIFEDKNFPITFTPINKTIHEGFVDNDLKICVFTDHQIFDRYH
                     KYSLKSQKTKAGKLVLSLKELMQFQVGDYVVHSEHGVGRFGGLIRTEINGKTQEVIKL
                     LYKDDDIIFVNIHSLHRISKYKSKEGEPPKINKLGTTAWVNLKEKTKKKVKDIARDLI
                     ALYAKRKSEKGFAFSPDSYLQNELEASFLYEDTPDQLKTTIALKKDMESDKPMDRLIC
                     GDVGFGKTELAIRAAAKAVADSKQVAVLVPTTVLAFQHFNTFKKRLKKFPCTVEYISR
                     ARKTSDVKLIIEKTKTGAVDILIGTHKLLSKELEFKDLGLLIIDEEQKFGVSTKEKLR
                     QLRSHLDTLTLTATPIPRTLQFSLMGARDLSVITTPPPNRYPVQTELIRFDDDIIREA
                     IEFELARNGQVFFVNNRVSNLPELEMQLKRLVPHARIAVGHGQMDSEKLEGVLLDFIH
                     HEYDILLATTIIESGIDIPNVNTMFINNAQYFGLSDLHQLRGRVGRTNKKAFCYLISP
                     SLAILPTDARRRLQAIETLADLGSGIHIAMQDLDIRGAGNILGAEQSGFITDLGYETY
                     HRILDEAVSELKHEEFADVFAEEIEHHMNYVADCMFESDLELLFPSDYV"
BASE COUNT         1306 a          680 c          748 g         1323 t
ORIGIN      
        1 taacaccccc tgccgctgca gctctactct cggtggggaa ctctcctgtg tgagtaagtc
       61 cagggtctct aaaatgcaca tgactatcaa tcactccagg caatacccag cagtcatctg
      121 catcaattgt gtggactttt tcaactatac tttttggaac ttcgtcttta taaatggcat
      181 caattttgcc atcttttata agaatagatg ctttgtaaat ttttccttcg tttacaatgc
      241 gaccgttgct gataagtaac ataatgtcaa tataaaatgt tctatttgtg ggtgattatt
      301 ttttttgtgg aaattttcta aagaagcttt cccaccgtag ttgtaatact ccgattacag
      361 cctctctaaa tattttacca ctcatttttg atgttcctaa ttcgcgattg ataaaaatga
      421 cagatacttc tttgatattg aatccgcatt taaaagcagt aaatttcatt tcaatttgaa
      481 aagcatatcc aacaaatttt attttatcca attcaatagt ttcgagcaca gctcttttgt
      541 aacatttaaa gcctgctgta gtatctgcaa tggctaagcc ggtaacaaaa cgtacatatt
      601 ttgatgcaaa atacgacatc aatacacggc tcatgggcca gtttactaca tttactccag
      661 ttacgtatcg agaacctatc gctacatctg cacctccttg agagcaggct tcgtacagtc
      721 gtaataaatc gttaggatta tgagagaaat ctgcatccat ttcatatata tactcgtaag
      781 cagaagccaa agcccagtta aatccagcaa tatatgctct acctaaacca tttttggctt
      841 ttcgctcaag taaatgtaat tgagtagaga actctacttg taaccgtttt acaatatctg
      901 ccgtaccatc aggagagttg tcatcaatga ctaaaatgtg aaacgatttt ggtaaagcaa
      961 aaactgcacg aataatattc tcaatatttt ctttttcgtt gtaggttggg ataagaacaa
     1021 ggctgtccga catgatagta tttactgaat ttgagtggtt attaatttgt tcccaaagat
     1081 acataaagga ttgtaaataa attgagattt tgtcttcatt tttttaatct ttaacctaaa
     1141 attgtgtact tttgcaaaaa tttttcagtg gatattaaag aattttcgca atcactcacc
     1201 tcacatcggg ttgtaaatgg cataaatcag tgggttgagt cttcacacaa aaacgtatgt
     1261 atacaaggtt tttgtggatc ttcatgtgct cttttttttg catcttcaat attagccaaa
     1321 acaaatacgg tatatatcct aaatgatgct gaggaggctg cgtattttta tcacgactta
     1381 ttgcaattat tatcccctga tgatgtgtat tattttcctt catcatacaa aaaacgagtg
     1441 cgtgaaggag accaaaaaga tgctgctaat caagtgttgc gtactgaagc tttaaacgat
     1501 atttctcaaa aacagtcttg gatacttgtt acttatcctg aagctattgc tgaaagagta
     1561 attcctcaac aagatttgtc aaaagagacg cttcatttgg ctgtgggaga aaaattggat
     1621 agcgattttg ttatttcatt gcttgttgaa tatggttttg aacgcgtaga ttttgtatat
     1681 gaaccagggc aatttgctgt gcgtggtagt attatagatg tgttttcttt ttcacatgat
     1741 gtccctttta gaattgattt ttttggagat actattgata ctattcgtac ctttgatatt
     1801 gaaagtcaat tgtcaatcga aaagaaaacg catatctcaa tcgtatcaaa tagcgtaaat
     1861 atagaagaaa atgccttagt tcctttattc aaatttttca cgaataaggt cgtctatact
     1921 ataaaaaata ccccatacgt agtagactta attgataaga taaaccaaga aaatgacgaa
     1981 acccgattta ttttatctac agaatggatt gaacaatggc aagggaataa agtaattgag
     2041 tttggaaatt cacctttctt ttcttctcct cacattatta cactcaatca gaatattcaa
     2101 ccaatatttc acaaaaattt tgacttactt gctgatagtc tgacggagta tatagcacaa
     2161 cagtatcaaa tctatgtatt gagtgataat cccaaacaaa cagaacgatt aaaagatatt
     2221 tttgaagata aaaattttcc aatcacgttt acacccatca ataaaaccat tcacgaagga
     2281 tttgtagata atgatttgaa gatttgtgtt tttacagatc atcaaatttt tgatagatac
     2341 cataaatatt ctcttaaatc gcaaaaaaca aaagcgggaa aattggtttt atcgctcaaa
     2401 gaattaatgc agtttcaagt tggcgattat gttgttcata gtgagcatgg tgtgggtagg
     2461 tttggtgggc ttattcgtac agaaattaat ggaaaaacac aagaagttat caaactgttg
     2521 tataaagatg atgacattat ttttgtaaat atccattcat tgcatcgtat ttccaaatac
     2581 aaaagcaaag aaggagagcc tccaaagata aataaattag gtacaacagc ttgggttaat
     2641 ctaaaagaga aaacaaagaa aaaggtaaaa gatattgctc gtgatcttat tgctttgtat
     2701 gcgaaacgca aaagcgaaaa gggttttgcc ttttctcctg atagttattt gcaaaacgag
     2761 cttgaagcat cttttttata cgaagataca cctgatcagc tcaagacgac tattgccttg
     2821 aaaaaagaca tggaaagcga caaaccaatg gacaggctta tttgtggtga tgtaggtttt
     2881 ggaaaaacag aattagcgat tagagctgcg gctaaagcag tagccgatag caaacaagtt
     2941 gccgtgttgg tgcccactac tgtattggca tttcagcatt ttaatacctt taaaaaacgc
     3001 ttaaaaaagt ttccgtgtac tgttgagtat attagtagag cccgaaaaac atcagatgta
     3061 aaattaatta ttgaaaaaac aaaaactggt gcagtcgata ttcttattgg aactcataaa
     3121 ttgttgagta aagagttaga atttaaagat ttaggattgc ttataattga tgaagaacag
     3181 aaatttggtg tttcgacaaa agaaaaactg cgtcaattac gctctcatct tgatacgctt
     3241 actcttacgg caacccctat tcctcgtact ttacaatttt ccttgatggg agctcgtgat
     3301 ttgtcagtga ttacaactcc accgcccaat cgctatcctg tacaaacaga gcttattcgt
     3361 tttgacgatg atattatccg cgaagccatt gaatttgagt tagcacgcaa cggacaagta
     3421 ttttttgtga acaatagggt gtccaatctg cctgaattgg aaatgcaact taaacgcttg
     3481 gttccacatg ctcgtatagc tgtaggtcat ggacagatgg attcggagaa actagaagga
     3541 gttttgttag attttattca tcacgaatat gatattttat tagcaactac cattatcgaa
     3601 tctggtattg atatcccaaa tgtaaatacc atgtttatta ataatgccca gtattttggg
     3661 ttgagtgatt tgcaccaatt acgaggcagg gtaggtagaa cgaataaaaa agcgttttgt
     3721 tacctaattt caccttcttt agccatattg cctacagatg ctcgcagacg cttgcaggca
     3781 attgaaacct tggcagattt aggtagtggc attcatattg ccatgcaaga tttggatatt
     3841 cgtggagctg gaaatatttt gggagcagaa cagagtggtt ttataacaga cttagggtat
     3901 gaaacatacc atcgcatatt agatgaagca gtgagtgagt taaagcacga agagtttgct
     3961 gatgtgtttg cagaagaaat agaacatcac atgaattatg tggctgattg tatgtttgaa
     4021 tctgatcttg agttgttgtt tccttcggat tatgtgc
//
LOCUS       DAOPUR010000080         4051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_22343_length_4051_cov_3.622873, whole genome shotgun sequence.
ACCESSION   DAOPUR010000080 DAOPUR010000000
VERSION     DAOPUR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22343_length_4051_cov_3.622873"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PKN90_02110"
     CDS             <1..1276
                     /locus_tag="PKN90_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNZ85105.1"
                     /translation="KRMRQFFRTSFFKHTATLLTANSLSQAIALLAYPLLTRIYAPDD
                     FGLFNLFLSIGGILAIIGTGNYHYAIVLPKEETKALACVKFSFILNLIVVGISSFFLL
                     FSSQIGKLFNSESLGEYLYLLPIFVLLTATWNILNYWFTRHQWYKPIAFYQISNSLGS
                     SILKYMLGIMGFLRGGLLIGTILAQVIAFAVGFIVAWKKGLSRTLHIQRQEIVETATT
                     YKNFPFYSLPHSLINTLGGSLPIIFLAPFFSLAEIGFFGMALTLAFRPINIISSSLHQ
                     VFYPITTQKVNDNDSIMPFFKRYFLRTFTLLIPLFGVLFFFLPMLTEWLLGNGWHQSG
                     KYIQLMLPWLALSAAVAPITYLADIFSQQRIALLLEILSFIVRFCALMIGIWQQNFYL
                     AIAYYCVASALIILVQLLWYWHLVKQYEIKRI"
     gene            1279..2442
                     /locus_tag="PKN90_02115"
     CDS             1279..2442
                     /locus_tag="PKN90_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ85106.1"
                     /translation="MRVLQLPSWYIPEGGMFCTEQSIALKQVGVEVHILANVALPWRK
                     YKSRIYSFPRKPFFTEENGITLLRYYAKRIPLADMWNMKLWVRNTLRLYDMYFEKFGH
                     PDIIHTHSSMWAGYAAALIKQRYGVPYVITEHRGRFSENSIESDELFPLSYHPFLYQA
                     FENAHSIIIVSEQLRKKVALYAGENVPIYTISNVVNTDFYTEKKEQNPIRPFTFINAN
                     SYHFAKAYDVLLDAFEKVYAQYPETTLLLLGNGFDDAQFVVQLNKLRSKNKIEFKGYQ
                     PPEGVLHYLHKSDSFVLSSRIEAQPVAILEAMSCGLPIVCTEVVPSEIVTPTIGVRCK
                     TDNADALAEAMIYMIEHHANFSSTDIRQFAVSVCSQKAVASQILAVYKQIIHG"
     gene            2435..3982
                     /locus_tag="PKN90_02120"
     CDS             2435..3982
                     /locus_tag="PKN90_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HNZ85107.1"
                     /translation="MVKLDKHNPISWTSANGVWVAGYCFDTEGNFYEKESLIALLQQI
                     TENHLESFVRSLNGIFACIIERDSETIIIMDKTRLIPLFYAIENSQVIVSNNPYTLFP
                     TSPVVDTSALNEYLYSSVVLDEKTLIKGIFQVQPSSYIICKNNHLDIHHYYTFAVKKH
                     EIAVAKDYNQQFETVLNDVFHRLLTSCKGKQVVVPLSGGYDSRLIVCMLAKLGYKNVL
                     CYTVGRPNNPELIIAQKVAKRLGYKHVFISNETPSYLDDNTFEAYYRYSGYFTNFFWM
                     YEYIGVKTLTEQNIIEKEAIFIPGHSGDFLAGSQLQKAGITLKTTLNEVEKLVLEQKF
                     AYGNGLKNAAIKQKMNDFIKQRKDYLPYSVFDDVDLLIKLPKNINNAARIYGFFGHQT
                     RLPFWDNEMIEFFKTLPIEEKQTQKFYHHYLTSKVFQPMNVAFVDELTYPEWRMKWYR
                     FKRQCKQLLPTHIQQYISNPPDYVCMKEISAPLKHQLPKTATNGMGGYNRVFLEWYWQ
                     QVKKIVD"
BASE COUNT         1294 a          746 c          713 g         1298 t
ORIGIN      
        1 caaacggatg cgtcagtttt ttaggacatc gttttttaaa cacacagcaa cattgcttac
       61 tgccaatagc ttatcacaag ccattgcctt attggcatat cctttactga cacgtattta
      121 tgcccctgac gatttcggat tgttcaatct atttttaagt atcggcggaa tattggcaat
      181 tattggaact gggaactacc attacgccat tgtgctacca aaagaagaaa caaaagccct
      241 agcttgtgta aaattctcct ttatactgaa ccttattgta gtaggtataa gtagtttctt
      301 cctactcttt tcatcacaga ttggcaaatt atttaattct gagtcattag gagaatacct
      361 gtatttactc cccatattcg tattattgac cgcaacatgg aacattctta actactggtt
      421 tacacgacac caatggtaca aacccatagc attttatcaa attagtaaca gcttgggttc
      481 ttcgatacta aaatatatgt taggtatcat ggggttttta cgtggtggtt tattaattgg
      541 cactatactt gcacaagtaa ttgcttttgc cgtaggtttt atagtggcat ggaaaaaagg
      601 tttgtcaaga actttgcata tacaacgaca agaaatagta gaaacggcaa caacatataa
      661 aaatttccca ttctattctt taccacacag cttaatcaac accttaggag gaagtttgcc
      721 aattatcttt ttagctccat ttttttcatt agcagaaata ggtttttttg gtatggctct
      781 tacacttgca tttcgcccaa tcaatattat ttctagttcg ctacatcaag tattctatcc
      841 tattaccacc caaaaggtaa acgacaatga ctccattatg ccatttttta aacgatactt
      901 tctacgaaca tttaccttgc ttataccctt atttggtgtg ctatttttct ttttacctat
      961 gcttaccgaa tggttactag gtaatggttg gcatcaaagc ggaaaatata ttcagttgat
     1021 gctaccttgg ttagccctat cagcagcagt tgcacccatt acatatttag cagatatttt
     1081 ttctcaacaa agaattgcct tgttgcttga aattctttca tttattgtac gattttgtgc
     1141 attaatgatt ggaatatggc aacaaaattt ctatcttgcc atcgcatatt attgtgttgc
     1201 aagtgcttta attatacttg ttcaactact ttggtattgg catctggtaa aacagtacga
     1261 aattaaacga atttgattat gagagtgtta cagttaccat cttggtatat ccccgaagga
     1321 gggatgtttt gtacagaaca aagcattgct ctaaaacaag ttggcgttga ggtacatatc
     1381 ttggctaatg tggcacttcc ttggcgaaaa tacaaatctc gcatttattc gtttcccaga
     1441 aaaccttttt ttaccgaaga aaatggtatt actcttctgc gttattacgc caaaagaata
     1501 cctttagctg atatgtggaa tatgaaacta tgggttagaa ataccttgcg actatatgat
     1561 atgtattttg agaaatttgg tcatccagat attattcaca cacacagtag tatgtgggct
     1621 ggatacgctg ctgcacttat caagcaacgt tatggcgttc cgtacgtaat taccgaacat
     1681 agaggtcgtt tttctgaaaa ttctattgaa tctgacgagc tatttccgtt aagctatcat
     1741 ccttttttgt accaagcgtt cgaaaatgca catagtatca tcatagtatc ggaacaactg
     1801 cgtaagaaag tggctcttta tgctggagag aatgttccta tttatacaat ttcaaatgtt
     1861 gtaaatacag atttctatac cgaaaagaaa gaacaaaatc ctatccgacc attcaccttt
     1921 atcaatgcca attcttatca ttttgccaaa gcctacgatg tattgctaga tgcttttgaa
     1981 aaagtatatg cacaatatcc tgaaacaact ttactgttat tgggcaatgg atttgatgat
     2041 gctcaatttg tagttcagct caacaaatta cgctcaaaaa ataaaataga atttaaagga
     2101 taccaaccac ctgaaggcgt attgcattac ttacataaaa gcgattcgtt tgttttatca
     2161 agccgaatag aggcacaacc tgtggctatt ttagaagcca tgagctgtgg attgcctatt
     2221 gtgtgtacag aggtggtgcc ttccgaaatt gttaccccta ctatcggtgt acgttgcaaa
     2281 acagacaatg cagatgcact agcagaagca atgatctaca tgattgagca tcatgccaat
     2341 ttctcatcta ccgatattag gcaatttgcc gtttctgtgt gtagccaaaa agctgtagct
     2401 tcacaaattt tggcggtgta taaacaaatt attcatggtt aaacttgaca aacataatcc
     2461 catctcgtgg acaagtgcca atggcgtttg ggtagctggc tattgttttg atacagaagg
     2521 aaatttttat gaaaaagaat cacttattgc gttactccaa caaatcacag aaaatcattt
     2581 agaatcattc gtacgttcgc ttaatggaat atttgcctgc atcattgaac gagatagcga
     2641 aacgattatt atcatggata aaacacgctt gattcctctt ttttatgcca tagaaaacag
     2701 ccaagtaatc gtatccaata atccatatac attgtttccc acatcgccag ttgttgatac
     2761 ctctgcactt aatgaatacc tgtattcttc ggttgtgttg gatgaaaaaa cactgattaa
     2821 agggatattt caagtgcaac catccagtta tattatttgt aagaataacc accttgatat
     2881 acatcattat tacacttttg cagtaaaaaa gcacgagata gctgtagcaa aagattacaa
     2941 tcagcaattt gaaacggtgc tgaacgatgt ttttcatcga ttacttacct cgtgcaaagg
     3001 gaaacaagta gttgtaccct tgagtggtgg ctacgattct cgcttaattg tatgtatgtt
     3061 agccaaactt gggtataaaa atgtgctttg ctatacagta ggcagaccca ataatccaga
     3121 gttaataata gcccaaaaag tagcaaaaag gctaggatat aagcatgtat ttatttcgaa
     3181 cgaaacaccc tcgtacctgg atgataatac atttgaggct tattatcgtt actctggtta
     3241 ttttaccaat ttcttttgga tgtacgaata tataggggta aaaacgctga ctgaacaaaa
     3301 catcattgaa aaagaagcca tatttattcc aggacattct ggcgattttc ttgctggcag
     3361 tcaattgcaa aaagcgggaa ttacgctgaa aactacattg aatgaggttg aaaagctagt
     3421 gctagaacaa aaatttgcgt atggcaatgg cttgaaaaac gctgctatta aacaaaaaat
     3481 gaacgacttt atcaagcaac gcaaagatta tctaccctat agcgtgttcg atgatgtaga
     3541 tttgcttata aaattaccta aaaatatcaa taatgcagca cgtatttatg gcttttttgg
     3601 tcatcaaact cgcttgcctt tttgggacaa tgaaatgatt gagtttttca aaacattacc
     3661 cattgaagaa aaacaaacac aaaaatttta ccaccattac cttacaagca aggtatttca
     3721 gcccatgaat gttgcatttg tagatgaatt aacataccca gaatggcgaa tgaaatggta
     3781 taggtttaaa aggcagtgca aacaattgtt acccactcac attcagcagt atatttccaa
     3841 tccgcctgat tatgtgtgta tgaaagaaat ttctgcacca ttaaaacacc aactaccaaa
     3901 aacagcaaca aatgggatgg gtggttacaa tcgggtattt ttagaatggt attggcaaca
     3961 ggtaaaaaag atagttgatt aagtagccta ccactctatc tttttcaaaa accatttttt
     4021 tgcgatatac atcaaaggac gtaaaaagct a
//
LOCUS       DAOPUR010000081         4038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_22439_length_4038_cov_2.334672, whole genome shotgun sequence.
ACCESSION   DAOPUR010000081 DAOPUR010000000
VERSION     DAOPUR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22439_length_4038_cov_2.334672"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4038)
                     /locus_tag="PKN90_02125"
     CDS             complement(<1..>4038)
                     /locus_tag="PKN90_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNZ85108.1"
                     /translation="SKDGSSPSRSNYSEVLELYYGYSMRKGTRDMKYLKTEIDEMRPV
                     YLELAGNPGHAVVIDGYKDENYLHLNFGWGGQANGYYMMNTNSTFWVGYTYGTNLGSN
                     IFYSPTPFITNESDSLALIAIHNALDGTTGWNTSLPVSKWHGVITHHSRVVELGLGGY
                     DYGVKGNIAPEIALLNKLQHLTIIGSELNGNLTADIFQLPDLESLHISKNAGTLQCEF
                     PSQIDGCQKLTDLSMPGILTGTIPESIGNLVQLQELSIYNGTLSGSIPLTISQLTKLK
                     TINISKNQLSGPIPDAFYNLVDVETLDLSENNLSGNLPISLEKMTQLKELRLNKNNLS
                     GILSDVFEHVKNISYLDVSENHLSGNVPESIGKLDKLQKLFLHNNQFVALPNDMSGLE
                     ALNELLVNNNLLTTLPLSINAISSLRTIDASNNQITTLHRDFGLLRNLQSLNISNNQL
                     TEIPSSIFLNAKLGTILCSNNKIKAFPSSIDFLPAYIDFAKFDDNELEGQIPLTLLSA
                     KASHVSLINNRFTYEDVPVSDELRHAVGVQKNHRLKQNKFPVMMGDTIRIDIRKISKL
                     SHPDNLYYWLEYPKYADKNGISFLEQLQPNPILTVVINEKTAAKKFYCKVFNNNVPTW
                     TFFFNNYEYINPTMSVVNTDTIEFEILSEDEMMAYIHAESNFIQSKQILNKTISDHMV
                     TLVPPLTTYRGSLVWQASTDGIAWHSITNTMANETIKGNIVSIKKDSLVISPLADAFY
                     RTAIVESDCNPLYSDTVHIKAYGKLLYDKEINTSNDDVKIELDDIEVLVPKGIHNTDF
                     RITIVEVANPPAKPDNALKMSPVYDINVSFANEFYLPISIKFKNIDKESFDHKNIDTY
                     VAMYFDDIAQEWVPFDKANISLQDTTLHLATYHFTKLAWFEMAHGSYTHIFTSKYVNV
                     IYKYGDGYPESNFYIRYNRQTDNQGRQYWHDTNNDPDADPYGTPYLIQDVAHYMDSII
                     HKFKNMGLETPSLRFNVYVGLIGNNGMIDAGGYLAGRGYFYIDPQYVSYFDNQEENRH
                     EMMRTLAHEYMHYTQDYYMTVLLQNYYWMEATAPIAARIVWEENSLPDTEPELLIQDA
                     LYPERDNTNLVQRILMMGRNKTIFETLSESWLSNYNIPVATKLRSNSADANIASLFLH
                     YMQNYREGKKLNATSLIKETSYLETWIEYLTSFVNKHLESTVGKEFDDYVRYVLSGQN
                     TKFSVLNSEGGNPFKHLIKNSESENTKAFALRQVYKFADTDKTPQKDEMSFTVPYLAA
                     KIVLLSNTSNKQLVVVNYKPSHEPNEHYKVYYGRYDSNLKKVEYTDISDSTSYNILLN
                     TRTVNAIDDARNTSFLLFVNTLAPSK"
BASE COUNT         1201 a          663 c          758 g         1416 t
ORIGIN      
        1 tttagatgga gctaatgtgt tgacaaacag taaaaagctt gtgtttcgag catcatcaat
       61 agcatttacc gttcgtgtgt tgagtaaaat attgtaggag gtagaatcag aaatgtcagt
      121 atattctact ttctttaaat tgctatcata gcgcccataa tacactttgt aatgttcgtt
      181 gggttcgtgc gagggtttgt aatttactac aactaattgt ttgttgctag tgtttgataa
      241 taatacgatt ttagcagcca aatagggtac cgtaaaagac atttcatctt tttgaggcgt
      301 tttatcagtg tcagcaaatt tgtatacttg acgcagtgca aatgctttgg tgttttcgct
      361 ttctgaattc ttaatgaggt gtttaaaagg attaccacct tcgctattta gtactgagaa
      421 tttggtgttt tgtccagata aaacataacg aacataatca tcaaattctt ttccaacagt
      481 actttctaga tgtttgttta caaagctagt taagtattca atccacgttt ctaaataact
      541 tgtttctttt atgagcgatg tagcgtttag ctttttccct tcacggtaat tttgcatgta
      601 gtgtaaaaac aaggaagcta tattggcatc agctgagttt gatcgaagtt tggttgccac
      661 aggtatgttg taatttgaaa gccacgattc actcaaggtt tcaaagatag ttttatttct
      721 acccatcatc aaaattcgtt gtaccaaatt ggtattatct cgttcaggat aaagagcatc
      781 ttgaatgagt agttcgggtt cagtgtcggg tagcgaattt tcttcccata caatacgtgc
      841 tgcaatcgga gcagttgctt ccatccaata ataattttgt aacaaaacag tcatgtaata
      901 atcttgtgta taatgcatgt attcatgagc caatgtacgc atcatctcgt gtctattttc
      961 ttcttggtta tcaaagtatg aaacatattg ggggtcaata taaaaatatc cacgaccagc
     1021 taaatagcct ccagcatcta tcattccatt attaccaatc aagccaacat acacattaaa
     1081 acgtaaactg ggtgtttcga gtcccatgtt tttaaattta tgaatgattg aatccatata
     1141 atgagctacg tcttgtatca agtatggcgt accatatggg tctgcatcag ggtcattgtt
     1201 cgtatcgtgc cagtactgac gaccttgatt atctgtttgc ctattatatc ttatgtaaaa
     1261 atttgattca ggatatccat caccgtattt atagatcacg tttacatact tggaagtaaa
     1321 aatatgtgta taggatccgt gagccatttc gaaccaagct aatttagtaa aatggtaggt
     1381 ggcaagatgc aaggtcgtat cttgtaaact tatattggct ttgtcgaaag gaacccactc
     1441 ttgggcaata tcatcgaaat acattgctac gtatgtatca atgttcttgt gatcaaagga
     1501 ttctttatca atatttttga atttaattga aatgggtaga taaaattcgt tagcaaaact
     1561 tacattaata tcgtaaacgg gactcatttt gagtgcatta tcaggttttg caggaggatt
     1621 agcaacttcg actatggtga tgcggaaatc ggtattgtgt atgcctttag gaactaatac
     1681 ttcaatgtca tctaactcaa tttttacatc atcattgctt gtgtttatct ctttatcgta
     1741 caataatttg ccataagctt ttatgtgtac tgtatcgcta tacaaaggat tacagtcgga
     1801 ttcaacaatg gcagtacggt aaaaagcatc agcaagtgga gaaataacca aagaatcctt
     1861 tttaatagaa acaatatttc ctttaatggt ttcatttgcc atcgtgttgg tgatagagtg
     1921 ccatgcgatt ccgtctgtag aagcttgcca caccaacgaa cctctgtaag tggttaatgg
     1981 aggaaccaaa gtaaccatat gatctgaaat tgttttgttt agtatctgtt tggattgtat
     2041 aaaattactt tcagcatgta tatatgccat catctcatct tccgaaagaa tttcaaattc
     2101 aatagtatct gtatttacaa cactcattgt tgggttgatg tactcgtaat tattaaaaaa
     2161 gaatgtccaa gtgggtacgt tattgttaaa cactttgcaa taaaactttt tggctgctgt
     2221 tttttcgttg ataaccaccg ttaaaattgg atttggctgc aattgttcta aaaaggaaat
     2281 tccattttta tctgcatatt ttggatattc taaccaataa taaagattgt ctgggtgaga
     2341 tagtttgcta atttttctga tatctatacg gatggtatca cccatcatca ccgggaattt
     2401 gttttgtttt aaacgatggt ttttttgcac cccaactgca tgacgtagct cgtctgatac
     2461 aggtacatct tcatacgtaa atcgattgtt tatgagcgat acatgcgatg cttttgcact
     2521 gagtagagta agaggtattt gtccctctaa ttcattatcg tcgaattttg caaagtcgat
     2581 atatgcaggt aaaaaatcta tagatgatgg aaatgcttta attttattat tggaacatag
     2641 gattgtacct agtttagcat ttaaaaatat tgagcttggg atttctgtga gttggttgtt
     2701 tgatatattc aatgattgaa ggtttcttaa taagccaaaa tcacgatgca aggtagtgat
     2761 ttggttgtta gaggcatcga ttgtgcgtaa acttgaaata gcattgatac tcaagggtaa
     2821 tgttgtgagt aaattattat tgacgagtaa ttcattcaat gcttctaatc cactcatatc
     2881 gttgggtaat gccacaaatt ggttgttatg taaaaacagt ttttgtagtt tatctagctt
     2941 accaatactt tcgggcacgt ttcctgataa atgattttcg gatacatcta aataactaat
     3001 attctttaca tgttcaaaca catctgatag gataccactt aggttgtttt tgtttagccg
     3061 taattctttc aattgagtca ttttttcgag agaaataggc agattccccg aaaggttgtt
     3121 ttcagataaa tcgagagttt ctacatctac caagttgtaa aaagcatcag gaatgggtcc
     3181 acttaattgg tttttactta tgtttattgt tttaagtttg gttaattgac ttattgttaa
     3241 aggaatactt ccagaaagag ttccattgta aatagataat tcttgtaatt gaactaggtt
     3301 tccgatggat tcaggtatgg ttccagtgag gatacctggc attgataagt cagttagttt
     3361 ctgacaacca tcaatttgac taggaaattc acattgtaat gtgcccgcat tttttgaaat
     3421 atgaagggat tcaaggtctg gtagttgaaa aatgtcagct gtaagattcc cattgagttc
     3481 gctaccaatg attgttaaat gttgtaattt gtttaataga gcaatttcag gagcgatatt
     3541 tccttttacc ccataatcgt atccacccaa tcctaattct accacccttg aatgatgtgt
     3601 tattactccg tgccacttcg atacaggtaa agacgtattc caccctgtag taccatcgag
     3661 tgcgttgtgt atggcaataa gagctaaaga atcagactcg tttgtgataa aaggagtagg
     3721 ggaataaaaa atattgcttc ctaagttggt gccataggta taaccaaccc aaaaagtact
     3781 attggtattc atcatataat acccattggc ttgaccaccc caaccaaaat ttaaatgtaa
     3841 ataattttca tctttgtacc catctataac tactgcgtgt ccaggattgc cagctaattc
     3901 gaggtataca ggacgcattt catctatttc agttttaagg tatttcatat cgcgagttcc
     3961 ttttctcatc gaatatccgt aatataattc aagaacttct gagtagttgg aacgtgatgg
     4021 agaactgcca tctttcga
//
LOCUS       DAOPUR010000082         4018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_22594_length_4018_cov_2.778703, whole genome shotgun sequence.
ACCESSION   DAOPUR010000082 DAOPUR010000000
VERSION     DAOPUR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22594_length_4018_cov_2.778703"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1465)
                     /locus_tag="PKN90_02130"
     CDS             complement(86..1465)
                     /locus_tag="PKN90_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ85109.1"
                     /translation="MIQKHSPLSLGTESIGKLLAQYAIPAIVAMTASSLYNITDSVFI
                     GHGVGALAISGLALTFPVMNLAAAFGSLVGAGASALMSMRLGQKDYDTANKVLGNVFV
                     LNIIMGLAFTGIMLVFLKPILYFFGASEATLPYAYDYLQVILYGNVVTHMYMGLNALL
                     RSSGHPQKAMYATICSVILNLGLTPLFIYVFGWGIKGSAWATVLSQLLMLIWQITIFS
                     NKKNFIHLKKGVFRLEKKIVLDSFAIGLSPFFMNAASSVIIIVINQALSRHGGDLAVG
                     AYGIVNRVVFLFAMVVLGLNQGMQPIAGYNFGAQHYDRVDKVLRTTILYATGIMIFGF
                     IIGQFFPFSVASVFTTDVQLTEHAVKGLRIVMIFFPIIGFQMVTGNFFQSIGQAKKAI
                     FLSLTRQMIFLLPCLLIFPSIWGVKGVWFSLPTADLASSLVAGWLLSKQLKQFKRSKQ
                     TKSCDEVGI"
     gene            1782..3044
                     /locus_tag="PKN90_02135"
     CDS             1782..3044
                     /locus_tag="PKN90_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNZ85110.1"
                     /translation="MKLNKLKRSLDLKDAVAVGLGAIIGAGIFVVTGVAAGVSGPSFI
                     IGLLIAGTIATFNGLSSSQLAAVYPQSGGTYEYGYRLLTPSLGFSAGWMFVLSKLSAG
                     GIVAIGFGSYFHQLIPYFSPIEYSIAAIVLLTFANLWGIQKVGTINIAIVLITLLSLL
                     YFVVGGIPSVDKTNFIPFAPFGWKGIAESAAILFFAFTGYARIATLAEEVRNPRKTIP
                     KAVIITIAVAILLYVGVSIVATGVLGAPSMASTSSPLQLAADRIQAPGMSVVITLGAS
                     TAMLGVLLSQIVGISRMLLAMGRRNDLPHFFESIHKKTAVPHIAILFTSAIILLITLF
                     GSFPFVVRTATFTILLYYTIANAAAIRQPRIEQIYGSIIPWLGLIGCIVMAFSLPCMV
                     IVSGFGLLLIGFIARYIYQYSSKIKNGE"
     gene            3296..4018
                     /locus_tag="PKN90_02140"
                     /pseudo
     CDS             3296..4018
                     /locus_tag="PKN90_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779098.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
BASE COUNT         1360 a          798 c          672 g         1188 t
ORIGIN      
        1 ataatgcgac ctccacttcc taattctcta ccaatggtaa taataaaatt gttgttcata
       61 gaaaaatcct cctaataacg ttgaattaaa tccctacttc atcacacgac ttagtctgtt
      121 tactcctttt aaattgttta agttgtttac taagcaacca tcctgcaacc aaacttgagg
      181 ctagatcagc tgtaggcaaa ctaaaccaaa cacctttcac gccccatatg gaagggaaaa
      241 taagcaaaca aggcaacaaa aatatcatct gcctagtaag tgaaagaaaa atggcttttt
      301 ttgcctgacc aatactttga aaaaaattgc ccgttaccat ctgaaatcca ataattggaa
      361 aaaaaatcat cacaatacgc aagcctttta ctgcatgttc ggtaagttgc acatcggtgg
      421 taaatacaga tgcaacagaa aatgggaaaa attgccctat aataaatcca aaaatcataa
      481 tgcctgtggc atacaaaata gttgtacgca gcactttatc tacacgatca taatgctggg
      541 caccaaaatt ataccctgca atgggttgca ttccctgatt aagtcccaat actaccatgg
      601 caaaaaggaa aacaacacgg ttaacaattc cataagcacc tactgccaaa tctccaccat
      661 gacggcttaa tgcctgattg ataacgataa taataacaga tgacgctgca ttcataaaaa
      721 aaggagataa tccaatggca aacgaatcga gaactatctt tttttctaat cgaaaaactc
      781 ctttcttcaa atgaataaaa ttctttttat tactaaaaat tgttatctgc catattaaca
      841 tcaagagttg tgacagtaca gtagcccaag cactcccttt aataccccat ccaaaaacat
      901 aaataaataa tggggtaagt cctaaattaa gaataaccga acatatcgtt gcatacattg
      961 ccttctgagg atgacccgat gaacgcaaca aggcattaag acccatatac atatgtgtta
     1021 ctacatttcc gtaaagaatt acctgaagat aatcatacgc ataaggcagt gttgcttcgc
     1081 tagcaccaaa aaaatacaaa ataggtttca gaaaaacaag catgatgcct gtaaatgcca
     1141 accccataat aatattcagc acaaatacgt tccctagtac tttattcgca gtgtcatagt
     1201 ctttttgacc taatcgcatc gacataagag cagaagctcc cgctccaacg agcgaaccaa
     1261 atgccgcagc taagttcata accggaaaag taagagctaa acctgatata gcaagagctc
     1321 caactccatg cccaataaaa acactatccg tgatattgta tagagaagaa gctgtcatcg
     1381 ctacaatagc aggaatagca tattgtgcca acagcttacc aatactttct gtaccaagac
     1441 ttagtggaga atgtttctga atcattgttg ttttttttgg aagaactaca aagttcgtga
     1501 aaagtttaca aatactactg attttttata cttttttcgg agaatattca acgtaacgat
     1561 atctcctttt ctattttcaa aatgaatggg caaacatacc atactagtca acgaaaaaaa
     1621 tagtacacac aaccaaccat actgtatatt cttcaaaaat accatttttt agtacaatta
     1681 taccatatag ttattctctt tttcaatact tttgtacata gataacaacg attttatact
     1741 tacagacatt gtggggatag ggttaaacaa acatacacac tatgaaacta aataaactaa
     1801 aaaggagttt agacttaaaa gatgccgtag ctgttggctt aggagcgatt attggagcag
     1861 gtatatttgt tgttacagga gttgctgctg gtgtatctgg accttctttt atcattggct
     1921 tattgatagc aggtacaata gccactttta atgggctcag ttcatcacaa ttggctgccg
     1981 tttatcctca atctggaggt acgtacgaat atgggtatcg cttgttaacc ccttcgttag
     2041 gcttttctgc aggatggatg tttgtattaa gcaaactatc agcaggaggc attgtggcta
     2101 taggttttgg aagctatttc catcaactta taccctattt ctctcccatc gaatattcta
     2161 ttgcagccat tgtattgctt acgtttgcca atttgtgggg catacaaaaa gtaggaacga
     2221 taaatattgc tattgtactc atcaccttat tatcactact atattttgta gttggtggta
     2281 ttccaagtgt tgataaaaca aatttcattc cttttgctcc atttggatgg aaaggaatag
     2341 ccgaatctgc agctatctta ttttttgctt tcacaggata tgcacgtata gccacattag
     2401 ccgaagaagt tcgcaaccca cgaaaaacaa tccccaaagc ggtgattata actatagccg
     2461 tggcaatact actatatgtt ggcgtatcta tagtggctac gggagtatta ggagccccat
     2521 ctatggcaag tacgagttct ccactacaac tagcagcaga caggatacaa gctccaggta
     2581 tgtctgttgt tatcacctta ggagcctcaa cagctatgtt gggcgtacta ctaagccaga
     2641 tagtaggtat tagccgcatg cttttggcaa tgggtcgccg caatgatttg cctcattttt
     2701 ttgaatcgat tcacaaaaaa acagcagttc ctcatatagc cattttattc acttcggcaa
     2761 ttattttgtt aatcactcta ttcggttctt tcccatttgt tgtacgtaca gccactttca
     2821 ccattctatt gtattacacc atagctaatg cagctgctat tcgtcaacct cgcattgaac
     2881 agatatatgg ctctataata ccttggttag gactgatagg gtgtatcgta atggcttttt
     2941 ctctcccatg tatggttatt gtttctggct ttggattatt gcttattggt tttattgcaa
     3001 gatatatata ccaatatagt agcaagataa aaaatgggga atagattcta ttcatataat
     3061 cgaaagcaat tcatcttcaa tataaaagca aaatacaccg ttttaagaca aattaactct
     3121 aataaatatc atttctagaa aatttgtcgc gtttttcagt tttttattga tattatttgt
     3181 atttttgcat ccgaaaaaaa agaaagcagc aataactcta taattaccct ttagtataca
     3241 ctgtttttct tttaaaaaag aataaaaatt ccccctaatt taataacaag cttttatgtc
     3301 aaaagccaca aaaatcatag ccactatcag cgacaagcgt tgcgatgtag attttctacg
     3361 ccaattatac aacgaaggaa tgaatgttgt gcggttaaac tcagcacacc tcgacttgga
     3421 gggattttta cgcatcataa acaataccag agaagttagc aaccaaattg ccatactgat
     3481 ggacaccaag ggtccagaaa tacgaacaac gattgtacaa ggcaatggca ttgttataaa
     3541 aacaggggat aaatcaaaat ttcaggcaat ccagataaaa tatctaccca cgagcatatt
     3601 tatgttaact atccgttttt tgttcgtgac ttgaatgtaa acgacgatat tttgttcgac
     3661 gatggtgaaa ttgacctaaa agtaattgaa aaaacaaccg attacctact atgcgaagcc
     3721 caaaacgatg gtatgctagg tagccgtaaa agcgtaaacg tacccggtgt acgcatcaat
     3781 ttaccctcat taaccgaacg ggacaaacaa aacatcttaa tagcaattga aaatgatatc
     3841 gattttattg cccactcttt tgtacgcaat gcacaagata taaaagacat tcaagctata
     3901 ctcgaccaac acaacagtcc aataaaaatt atctcaaaaa tcgaaaacca agagggggtt
     3961 gataatatca acgaaatatt ggaacaaacg tatggggtaa tgattgctcg tggtgatt
//
LOCUS       DAOPUR010000083         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_22815_length_3989_cov_3.450686, whole genome shotgun sequence.
ACCESSION   DAOPUR010000083 DAOPUR010000000
VERSION     DAOPUR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22815_length_3989_cov_3.450686"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..2151
                     /locus_tag="PKN90_02145"
     CDS             205..2151
                     /locus_tag="PKN90_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNZ85111.1"
                     /translation="MISIEGLTVEFGAKALFDNISYVVNKKDRIALVGKNGAGKSTML
                     KIIAGLQEPTRGQCIIPKDLTIGYLPQHMIHNDDATVYEEAEKAFAHILEIQDQIEKN
                     NILLCERDDYESDSYHSLIEEITHLNEQLAMLGGVNYVADIERTLFGLGFLRTDLARP
                     TKEFSGGWRMRIELAKLLLQRPDVLLLDEPTNHLDIESIQWLENFLASRANAVMLVSH
                     DKAFINAVTTRTIEISLGKIYDYRANYSTYLELRKERREQQLRAYENQQKQIQDTEEF
                     IERFRYKTSKAVQVQSRIKQLEKIDRLEVDEEDTTSLRLKFPPAPRSGSFPVIAEGLA
                     KLYGNYVVFSNVDLTIKRGEKVAFVGKNGEGKSTLVKCIMNEIEYAGKLTLGHNVQIG
                     YFAQNQASLLNESKSVFETIDDVAVGDIRTKIRDILGAFMFGGEEADKKVKVLSGGER
                     SRLAMIRLLLHPVNLLILDEPTNHLDIRSKDVLKQAIKEFDGTVIVVSHDREFLDGLV
                     TKVYEFADKKIIEHIGGVYEFLHKKKLSSVQEIERKVLPNKETISSVQLETDQKLSYE
                     ERKERIKNLRKAEKLVETCEQDIAKLEKELETIEQAMLQPENAANVSFVASYTQKKRA
                     LEQKYYEWELLAEDVETKKNELSN"
     gene            2175..2483
                     /locus_tag="PKN90_02150"
     CDS             2175..2483
                     /locus_tag="PKN90_02150"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNZ85112.1"
                     /translation="MQFNNVSIEKKANVYFNGNVTSRTVLFPDGTRKTLGIMMPGEYE
                     FGTDSAELMEMLGGNMDVLLPNESEWKSYAEGDSFHVPAKSSFKLKVNTVVDYCCSYL
                     "
     gene            complement(2492..3304)
                     /locus_tag="PKN90_02155"
     CDS             complement(2492..3304)
                     /locus_tag="PKN90_02155"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ85113.1"
                     /translation="MVQNKIVLITGASSGIGLVTAVQLAEQGYIVFASSRRGNSSVNL
                     PNLHFVQADVNDKDQMKNVIELIVKQYKKIDVVICNAGNGIAGAIEDCSSDEIRFQME
                     TNFYGVVNTIQQCLPYLREQKNGKILVISSLAAIFPIPYQAFYAASKAAVHILTQALS
                     IELKRFNIQVCSILPGDTKTNFTKARSYAVQAQSPTSVYKEKMLASVAKMEQDEQNGM
                     KPDNVAKTIVKQVMAKKMGKVIVPGISNKILYGLAHILPTKLLLALLHKLYN"
     gene            complement(3364..>3989)
                     /locus_tag="PKN90_02160"
     CDS             complement(3364..>3989)
                     /locus_tag="PKN90_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNZ85114.1"
                     /translation="SRSGVLISANSEKKEKDPCNCYPEITPKRPKELECEIVRFQNNK
                     EKWIAFVGLLDGRPYEIFTGLADDDDGILLPKNITSGKIIRNYNEETGVKSYDFQFSN
                     KRGYKTTIEGLSHKFNPEYWNYAKLISGVLRYGMPIDQVVRLVNGLQLNNESINTWKN
                     GVERALKKYIPDGTQAKGQVCPKCGDTLVFEEGCLICKKCGYSKCGS"
BASE COUNT         1318 a          624 c          768 g         1279 t
ORIGIN      
        1 tgtatcttga ttattggttg tttatcaaaa tatcagtcag atcctcttta tatgtgtggt
       61 aaaatacaga agcaacttgt tgtaaaaatg gtttcataag tagtatacta aggatagcaa
      121 caaaaataca tataatattg cgaaaaaaac gattacaatg agattaaaat ttctgtattg
      181 aaaaatacca tttatctttg cattatgatt tcaatagaag gacttacagt agaatttggt
      241 gcaaaagcat tgtttgataa catatcatat gttgtaaaca aaaaagacag aatagcattg
      301 gttggtaaaa atggtgctgg aaaaagtaca atgcttaaaa ttattgcagg cttacaagag
      361 cctacacgtg gtcagtgcat tattcctaag gatttaacca ttggttattt accacagcat
      421 atgattcaca acgatgatgc caccgtatat gaagaagctg aaaaggcttt tgcccatatt
      481 ttggaaattc aagatcaaat agagaaaaat aatatattac tatgcgaacg agatgattat
      541 gaatctgatt cataccattc gcttattgaa gagattacac atctcaacga acaacttgct
      601 atgttgggtg gggtgaatta tgtggctgat attgaacgaa ccttgtttgg tttaggattt
      661 ttacgcacag atttggcacg acctaccaag gaatttagtg gtggttggcg tatgcgaatt
      721 gaattagcaa aattgttgct ccaacgtcct gatgtattac tgttagatga gccgacaaat
      781 catttggata tagaatcaat acaatggtta gaaaattttt tggcaagtag agccaatgcg
      841 gttatgttag tttcgcatga taaagccttt attaatgccg taactacacg cacaattgaa
      901 attagtttgg gaaaaatata cgattataga gctaattatt caacgtacct tgaacttcgc
      961 aaagaacgta gagaacaaca attgcgtgcg tatgaaaatc aacaaaagca aattcaagat
     1021 acagaagaat ttattgagcg ttttcggtac aaaacctcta aagctgttca ggtgcaaagt
     1081 agaataaagc aacttgaaaa aatagataga ttagaagttg acgaagaaga tacaacttca
     1141 ttgcgtttaa agtttcctcc agcaccccgt tctggaagtt ttcctgtaat tgctgaaggt
     1201 cttgccaaat tatatggcaa ttatgttgtg ttctcaaatg tcgaccttac tattaaaaga
     1261 ggagaaaaag tggcatttgt aggaaaaaat ggagagggaa agtcaacttt ggtgaaatgt
     1321 atcatgaacg agattgagta tgctggtaag ttgactcttg ggcacaatgt gcaaataggt
     1381 tattttgctc aaaatcaagc ttctctgtta aacgaatcga aatctgtttt tgaaaccatt
     1441 gatgatgtgg ctgttgggga tattcgtaca aaaattcggg atatattagg tgcatttatg
     1501 tttggtggcg aagaggctga taaaaaagta aaagtgctct caggaggaga acgcagtcgg
     1561 ttggcaatga ttagattatt gttgcatcca gttaatttgc ttattttaga tgagccaaca
     1621 aaccatttag atatacgttc aaaagatgta ttaaaacaag ctatcaaaga gtttgatgga
     1681 actgtgattg tggtttctca cgatagagaa tttttagatg gtttggtaac gaaggtgtat
     1741 gaatttgctg ataaaaaaat tattgaacat ataggcggtg tgtatgaatt tttacataaa
     1801 aagaaactta gttctgtgca agagattgag agaaaggttc tgcccaataa agaaacaatt
     1861 agtagtgtgc aacttgaaac tgaccagaaa ttatcttatg aggaacgcaa agagcgtatt
     1921 aaaaacctac gaaaagcaga aaaattagta gaaacttgcg aacaggatat tgcaaaactc
     1981 gaaaaagagc tggaaacaat agaacaagcc atgttgcaac ctgaaaatgc agctaatgtt
     2041 tcttttgttg cgtcgtatac ccagaaaaaa cgagctttgg agcaaaaata ttatgagtgg
     2101 gaattattgg cagaagatgt ggaaacaaaa aaaaatgaat tatcaaatta atataactat
     2161 caaatccgtt tattatgcaa tttaacaacg taagtattga aaaaaaagcc aacgtatatt
     2221 ttaatggcaa tgttactagc cgaacagtat tatttcctga tggtacacgt aaaaccctag
     2281 gtataatgat gccaggagag tatgagtttg gaacagatag tgctgaatta atggaaatgt
     2341 tgggaggtaa tatggatgta ttactaccta acgaatcaga atggaaatct tatgctgaag
     2401 gagattcgtt tcacgtacct gctaaatcgt cgttcaagtt aaaagtgaat acggttgtag
     2461 attattgttg ctcttatttg taggttacat gttagttata caatttatgc agtagtgcca
     2521 gcaatagttt ggttggtaat atatgagcta atccgtacaa gattttattc gaaatacctg
     2581 gtacgattac tttccccatt tttttagcca ttacttgttt tacaatagtt tttgctacat
     2641 tatcgggttt cattccgttt tgttcatctt gttccatttt ggcaacagag gcaagcattt
     2701 tttctttata tacagaagta ggggattggg cttgtacggc atacgaacgt gctttggtga
     2761 agtttgtttt cgtatctcca ggcaagatgc tacatacttg aatgttaaaa cgctttaatt
     2821 caatcgaaag agcttgtgtt agtatgtgta cagctgcttt gcttgcagcg tagaatgcct
     2881 gatagggtat ggggaatatt gcagccaacg aagaaataac tagtattttt ccattctttt
     2941 gttcacgtaa ataaggtaaa cactgttgaa ttgtatttac tacaccatag aaatttgttt
     3001 ccatttgaaa acgaatttca tcactactac agtcctcgat tgcccctgca attccattgc
     3061 cagcattaca aataacaaca tcaatttttt tgtattgctt tacgattagt tcaataacat
     3121 ttttcatttg atccttgtcg ttgacatcgg cttgcacaaa atgcaggtta ggaagattaa
     3181 cagatgagtt tcctcttcta gagcttgcaa agactatata cccttgttca gcaagttgaa
     3241 cagcggtaac tagtcctata cccgaactag cacctgtaat taacactatt ttattctgta
     3301 ccatacaaga aattaacatg ttagggcggt tattacacac cctaacatag tagattaagt
     3361 agattagcta ccacattttg agtaaccaca ttttttacaa atcaaacacc cttcttcaaa
     3421 aactaaagtg tcaccgcatt ttgggcatac ttgtcctttt gcttgggttc catcaggaat
     3481 gtattttttt aatgctcttt caacaccatt tttccaagtg ttaattgatt cgttgttaag
     3541 ttgcagacca ttaactaatc taacaacctg atctataggc ataccataac gtaatactcc
     3601 agaaattagt ttggcataat tccaatattc tgggttaaat ttatgtgata aaccttcgat
     3661 agttgtttta tatccacgtt tgttagagaa ttggaaatca taactcttaa ccccagtttc
     3721 ttcattgtaa ttacgaataa ttttcccact cgtaatgttt ttaggtaata aaatgccatc
     3781 atcatcgtca gctagccccg tgaaaatttc gtacggtctt ccatctaata atccaacaaa
     3841 agcaatccat ttttctttgt tgttttggaa acgaacaatc tcgcattcca attctttagg
     3901 gcgtttgggt gttatttctg gataacaatt gcatggatct ttttcttttt tctcactgtt
     3961 agcagagata agaacaccag agcgtgagc
//
LOCUS       DAOPUR010000084         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_23003_length_3967_cov_2.882924, whole genome shotgun sequence.
ACCESSION   DAOPUR010000084 DAOPUR010000000
VERSION     DAOPUR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23003_length_3967_cov_2.882924"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..553)
                     /locus_tag="PKN90_02165"
     CDS             complement(5..553)
                     /locus_tag="PKN90_02165"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNZ85115.1"
                     /translation="METSPNNSKVDTIISSIIQPALIAKNCTRIFLVGFMGTGKTTFG
                     KKLSQKIHFSFIDTDLYIENNFHKTINEIFAEKGEEGFRTIETKILAEIAEIENVVIA
                     TGGGTPCFNNNMALMNSKGLTIYLETPTPDIAQRLITGKTKRPLLKDKTSEEIVEFIE
                     EILPKRSTYYKKADLTINVVEN"
     gene            complement(484..1917)
                     /gene="cls"
                     /locus_tag="PKN90_02170"
     CDS             complement(484..1917)
                     /gene="cls"
                     /locus_tag="PKN90_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNZ85116.1"
                     /translation="MEFLSDILILIFLATYVYTVVSSIVVVLLENKHPVRTSAWIMVL
                     IFVPFIGLLLYVVVGRSFHRQGNISKKLKNKILSFGTRQSIKEICAENNICSKHERLI
                     HLLRNSTDASIFTHNSVNIYTDGKQLFDDILSAMDNATAHIHAEYYAVETDETGNRFK
                     DMLIKKSKQGLEVRLIYDDVGSWRLKRSTIQEMKNAGVILQTFFELRFPYFTNKLNYR
                     NHRKILIIDGKIGFLGGVNIADRYTKGLPWGIWRDTHIKIQGNAVDGLQQLFLNDWLY
                     ITKKAIHHKKYFAIHDQYNKLPIQIVGSGPDSMWEGIMQGFCAAIQNASKYVYIQTPY
                     FLPNDSISNALESAALSGIDVRIMIPSRSDTRISLEASLSFVKTMLAAGVRVYQYQAG
                     FIHAKSIAIDNELAIIGSANMDFRSFEQNFEVSAFMYDSDKSMELRNIFLHDMKQCKR
                     LQYTQWKHRPITQKLIQSSARLFSPLL"
     gene            complement(1917..>2336)
                     /locus_tag="PKN90_02175"
     CDS             complement(1917..>2336)
                     /locus_tag="PKN90_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNZ85117.1"
                     /translation="PSLRTRRWFGKNPVRIGIDKNLRIPKNYLLYNGQTTTLLFNSIK
                     QEEGNPCMIQIDFTKDIIPQLLHELYKRNIHSLIVEGGKILLDSFINTGLFDEIHEET
                     ADVLLYEGTCAPQFSESILCSMEIVGKNTCATYVKKE"
     assembly_gap    2337..2346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2347..2933)
                     /gene="ribD"
                     /locus_tag="PKN90_02180"
     CDS             complement(<2347..2933)
                     /gene="ribD"
                     /locus_tag="PKN90_02180"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNZ85118.1"
                     /translation="MSIHETYMHRCLQLASLGKGRVAPNPMVGCVIVHHDVIIGEGYH
                     RTYGEAHAEVNAIESVKNKALLKESTLYVSLEPCSHYGKTPPCADLIIQYKIPRVYIG
                     TKDPNPKVSGNGIARLETAGVAVECGILATQAMEINKYFFTFQLKNRPYITLKWAKSA
                     DGYIDALQPKPIQLSNEITKTLVYKLRSENMAILVG"
     gene            3006..3869
                     /gene="prmC"
                     /locus_tag="PKN90_02185"
     CDS             3006..3869
                     /gene="prmC"
                     /locus_tag="PKN90_02185"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNZ85119.1"
                     /translation="MDKIAISDCKLFFTQSLSSLYPPNEIESLFRWTISHVLGIPFSH
                     TIAYKDIKITNTQEIDIKTIVKKLQQTQPIQYIFGETEFFGLSFHVNSDVLIPRPETE
                     ELVEWILADHKQESASKRILDIGTGSGCIAISLAKNIPTSRVTALDVSHNAIEVAKRN
                     AHNHELTIQFIEKDILHLKNGDLNEQFDVIVSNPPYVCENEKSTINNHVLLHEPHLAL
                     FVPNDNPLQFYEAIALFAHTNLAINGAVYVEINYLFGKETQQLFHQYGFSTHLKKDIA
                     GKDRMIKAWKN"
BASE COUNT         1203 a          708 c          653 g         1393 t
ORIGIN      
        1 tcttctaatt ttcaaccaca ttaatagtga gatcggcttt tttgtaatac gtacttcttt
       61 taggtagtat ttcttctata aattccacta tttcttcaga agttttatct tttaataaag
      121 gtcgttttgt ttttcctgta atcaaacgtt gtgcaatatc aggagtgggt gtttctaaat
      181 agatggttaa tcctttacta ttcatcaatg ccatattatt gttaaagcac ggggtacctc
      241 ctcctgttgc aattactacg ttttcaattt ccgcaatttc tgctaatatt ttcgtctcaa
      301 ttgtgcgaaa cccttcttct cctttttcag caaaaatttc attaatggtt ttatgaaaat
      361 tattttcgat atataaatct gtatcgataa acgaaaaatg aattttttgg gatagttttt
      421 tcccaaatgt tgtttttcca gtgcccataa agccaactaa aaaaattctg gtacagtttt
      481 ttgctataag agcgggctga ataatcgagc tgatgattgt atcaactttt gagttattgg
      541 gcgatgtttc cattgtgtat attgtaaacg tttacattgt ttcatatcgt gtaaaaagat
      601 attacgtagt tccatacttt tgtcagagtc gtacataaat gcacttactt caaagttttg
      661 ttcaaaactc ctaaaatcca tgtttgcact tccgataatt gcaagttcat tgtcaatagc
      721 aatgcttttt gcgtgaataa aacctgcttg atattgatat acacgcactc cagcagctag
      781 catggtttta acgaaagata aagaagcttc taatgatata cgggtgtcag aacgacttgg
      841 aatcataatt ctaacgtcta ttccagataa tgctgcagat tctaatgcat tggatatgct
      901 gtcattaggt aaaaaataag gtgtttgtat atacacatat ttggaggcgt tttgtatagc
      961 ggcacaaaat ccttgcatta tcccttccca catcgaatct ggaccacttc ctactatttg
     1021 aatgggtagt ttattgtatt gatcgtgtat agcaaaatat tttttgtgat gtattgcttt
     1081 tttggtaata tataaccagt cattcagaaa taattgttgt aatccatcta ctgcatttcc
     1141 ttgtattttg atatgcgtat ccctccatat tccccaaggc aagccttttg tatatctatc
     1201 tgcaatattt acacctccta aaaagccaat tttcccatca atgattagta tctttcggtg
     1261 gttacgataa tttaatttat ttgtaaaata ggggaatcgt aattcgaaaa atgtttgcaa
     1321 tatcacgcct gcatttttca tctcttgtat ggtggatcgt tttaatcgcc agctaccaac
     1381 atcgtcatat attaatcgca cttccagtcc ttgtttcgat tttttgataa gcatatcttt
     1441 aaagcgattg cctgtttcat cggtttcaac ggcataatat tcggcatgga tatgtgcggt
     1501 agcgttatcc atagcactga gtatatcgtc aaataattgt ttcccatcgg tataaatatt
     1561 gacactatta tgtgtaaata tggaagcatc tgtactgttt ctcaataaat gaattaatct
     1621 ttcgtgttta ctacaaatgt tattttcagc acaaatttct ttaatagatt gccttgtccc
     1681 aaaagataaa attttgtttt ttagcttttt tgatatattt ccttgtctat gaaaacttct
     1741 tcccacaaca acatataata atagtcctat aaatggaaca aaaataagta ccataatcca
     1801 cgccgaagta cgaacaggat gcttgttttc taacaataca accacaatgc ttgataccac
     1861 cgtatacaca tacgtagcaa ggaatatcaa tatgagtatg tcggataaaa attccattat
     1921 tcttttttta cgtaagttgc acatgtattt ttgccaacta tttccataga acataaaatt
     1981 gattcggaaa attgtggagc acaagtacct tcgtacaata aaacatctgc ggtttcttcg
     2041 tgaatttcat caaacaatcc tgtgttaata aaggagtcta ataaaatttt accgccctct
     2101 acaatcaacg aatggatgtt ccgtttatac aattcgtgta acagttgggg tataatatct
     2161 ttggtaaagt ctatttgtat catacacggg ttaccttctt cttgcttgat tgagttaaaa
     2221 agtaaggttg ttgtttgccc attgtaaagc aagtaatttt taggaatacg taaattttta
     2281 tctataccta tacgtactgg gttttttcca aaccaccgcc tagttcgtag cgatggnnnn
     2341 nnnnnnccaa ccaatatcgc catattttca gaacgtaatt tataaaccag tgtttttgtt
     2401 atttcatttg acagttgtat tggctttggt tgtagggcat caatatatcc atcggcactt
     2461 tttgcccatt tcaacgtaat atatgggcgg ttttttagtt gaaatgtaaa aaaatatttg
     2521 ttgatttcca ttgcctgtgt tgctaatatc ccacattcca cagcaacacc tgctgtctcc
     2581 aaacgtgcaa taccattgcc tgatactttt gggttagggt ctttcgttcc aatatataca
     2641 cgaggaattt tatactggat gataaggtct gcacaaggcg gagttttacc gtaatgcgaa
     2701 cagggttcta agctaacata aagtgttgat tcttttaata atgccttatt ctttaccgat
     2761 tcaatggcgt ttacttctgc atgtgcttct ccatacgtac gatggtatcc ttctcctata
     2821 attacatcgt gatgtacaat aacacatcca accattggat tgggagctac tctgcctttg
     2881 cctaaggagg ctaattgcaa gcaacggtgc atatatgttt cgtgaatact cattttattt
     2941 ggcttgaatg atgtccttac gttaaatttt tgtagttttg tattgtcgtt tgtaatacta
     3001 tatcaatgga taaaattgct atctctgatt gtaaattgtt ttttacccaa agtctttctt
     3061 ctttatatcc tcccaatgaa atagaaagcc tatttcgatg gacaatctcg catgtattgg
     3121 gtattccttt ctctcatacc attgcataca aagatataaa aattactaac acgcaagaaa
     3181 ttgatattaa aacaattgta aaaaaattac aacaaacaca accgatacaa tatatttttg
     3241 gtgaaacaga gttttttgga ttatcattcc atgttaattc ggatgtgctc ataccacgtc
     3301 ccgaaacgga agagttggtc gaatggattt tagccgatca taagcaagaa agtgcatcca
     3361 aaagaatact tgatataggt actggaagtg gatgtatcgc catttcgtta gcaaaaaata
     3421 tcccaacttc tcgtgtaaca gctctagatg tgtcgcacaa tgccattgaa gtagcaaaac
     3481 gtaatgcaca caaccatgag cttacaatcc agtttattga gaaagatata ttacatctaa
     3541 aaaacggcga tttaaacgaa caatttgatg ttattgtcag taatccgcct tatgtttgtg
     3601 aaaacgaaaa atccactatc aataatcatg ttttgttgca cgaaccgcat ttggctttat
     3661 ttgttcctaa cgataatcct ttgcagtttt acgaagcaat tgctctattt gcccatacga
     3721 atttagcaat aaatggagct gtatatgtgg aaattaatta cctctttgga aaagaaacac
     3781 aacaactttt ccaccagtat ggctttagta cacaccttaa gaaagatatt gctggcaagg
     3841 atagaatgat aaaggcatgg aaaaactaga cagaataaaa tctcccattt ttttaaaaac
     3901 agctctctat tgttcttcgg ctgaaagatg caaaagcgag gtaatagaac gtttaaagaa
     3961 agagacc
//
LOCUS       DAOPUR010000085         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_23009_length_3966_cov_3.085400, whole genome shotgun sequence.
ACCESSION   DAOPUR010000085 DAOPUR010000000
VERSION     DAOPUR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23009_length_3966_cov_3.085400"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKN90_02190"
     CDS             complement(<1..394)
                     /locus_tag="PKN90_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNZ85120.1"
                     /translation="MMIDEKYLFAFALTLFAGLATGIGSLMAFFSKKFNPNFLSAALG
                     FSAGVMIYVSFVEIFFKAKESLTASLGDVTGYWMTVVSFFAGIALIALIDKLVPSGEN
                     PHEISNIEEANYKKSTSNDKLLRMGLFSA"
     gene            667..1875
                     /locus_tag="PKN90_02195"
     CDS             667..1875
                     /locus_tag="PKN90_02195"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNZ85121.1"
                     /translation="MSSNQKATAWVVTSSAMGINLILGLLYSWSVIKAALVAEGWTNT
                     EASLPYTVCVALLAFMTVFGGKLQDEFGPRVVALGGGILFGVGIFLSAFVHTPWMMVL
                     TFGVVAGLGMGFAYSAATPAAIKWFEPRKKGLIAGIVVAGIGISSIYMAPLTNYLLQG
                     NTVQGTFMILGIIAIVFLSIFGAILHNPPANYIPKAKEGAKVVAHSADFTWQAMMKRK
                     PFYLLWATYFLSATAGLMLIGHIVSIARVQTGNPELNAFWLVMIFAAFNTSGRVLGGF
                     LSDKMGRTTGLLIVFLLQAINMFVFSFYTSFSLLVVGFAVAGIAYGALFALFPAVTAD
                     FFGVKNLGVNYGIIFTSWGIAGIVGPILGGMVADITGTYAGSYIVAGIMLLLGALLVK
                     LIKTPQQEIK"
     gene            2001..2981
                     /locus_tag="PKN90_02200"
     CDS             2001..2981
                     /locus_tag="PKN90_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HNZ85122.1"
                     /translation="MKAITQNSIGNAETMYIGEVDIPVLRENEVMVQVKAFGVNRADI
                     LQRKGKYPSPAGASPLMGLEVAGIVAEAPVSSNWKIGDKVFGLIPGGGYASYVAIDAE
                     MLWRMPDTMSFEEAAAIPEAFLTAFLALQWQGKLQRGQKVLLHAASGGVGTAAIQLAN
                     AMQAEILVTASASKHAICAKLGATVCIDYKTESWVDVVQEVMGKNSIDVVVDFLGASY
                     FNDNISVLAMDGRMVMLALMGGADVSGVDLRKIIGKRITVIGSTLRNRPLSFQRKLAR
                     EFAEQFLPSFSDGRLQPIIDTVMDWTLIKEAHERMESNQHVGKIVMKVSE"
     gene            3143..>3966
                     /locus_tag="PKN90_02205"
     CDS             3143..>3966
                     /locus_tag="PKN90_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNZ85123.1"
                     /translation="MAKVNKEDALRYHAEGKPGKIEVVPTKPYSSQRDLSLAYSPGVA
                     EPCLEIEKDASKAYDYTAKGNLVAVISNGTAVLGLGDIGAIAGKPVMEGKGLLFKIFA
                     GIDVFDIEVNEKDPKKFIEVVKAIAPTFGGINLEDIKAPECFEIEDALKRDLDIPIMH
                     DDQHGTAIISAAGLINALELTNKNIEDVKIVVNGAGAAANSCASLYIQLGAKLENIVM
                     LDSKGVINKQRTDLNDRKKPFATDRTIKTLTEALAGADVFLGLSVEGVLSADMIRT"
BASE COUNT         1255 a          584 c          857 g         1270 t
ORIGIN      
        1 aagctgaaaa taaacccata cgaaggagtt tgtcattact cgtacttttt ttgtaattag
       61 cttcttcaat attgctaatt tcatgagggt tttcgccaga aggaacaagt ttatcaatga
      121 gtgcaattaa ggcaattcct gcaaaaaatg aaacaaccgt catccaatag ccagtaacat
      181 ctcctaatga agccgtaagc gattcttttg ctttgaaaaa aatctctaca aaagatacat
      241 aaatcattac tcctgctgaa aaacctaagg cagctgataa aaaatttgga ttaaactttt
      301 tagaaaaaaa agccattaag ctcccaatgc cagttgctaa ccctgcaaat aaagttaatg
      361 caaaagcaaa taaatatttt tcgtcaatca tcatacgtca atagtctata attttacatc
      421 aaaagtatga aaaatattgc aataaaaaaa cctttttatc ttgttaagct aaaattcatt
      481 tttattttgt ttgattgtaa gaaaacaaac gtttgcgttg tttttattaa tgtatctttc
      541 agtaaaatac taactaatta aaataaaata gttttctttg catcgatttt gtaaaatgcc
      601 ttattctgtg ttcgaagaac tatttatata attactagta actaaaacat taaaatcaaa
      661 aaatcaatga gttcaaatca aaaagcgaca gcttgggtgg taacatcctc tgctatgggg
      721 attaatttaa ttttaggatt attgtattca tggagtgtaa ttaaagcagc attagtagca
      781 gaaggttgga caaatacaga agcctctttg ccttatacag tatgtgttgc attattagct
      841 tttatgactg tttttggagg taaattacaa gatgagtttg gtccacgtgt tgtagctttg
      901 ggtggtggaa tattatttgg tgtgggtatt ttcttgtctg catttgtaca tacaccttgg
      961 atgatggtgc ttacatttgg tgtggtagct ggtttgggaa tgggttttgc ttattcggca
     1021 gcaacacctg ctgctatcaa atggtttgaa cctcgcaaga aaggtttaat tgcaggaatt
     1081 gttgttgcag gaattggaat ttcttctatc tacatggctc cgttaactaa ttatttgtta
     1141 caaggaaata ccgtacaagg tacatttatg attttgggta ttattgccat cgttttttta
     1201 agcatttttg gagctatttt acacaaccct cctgctaatt atatacctaa ggcaaaagaa
     1261 ggggcgaagg tagtagctca ttcggctgat tttacctggc aagccatgat gaaaagaaag
     1321 ccattttatt tgttgtgggc gacttatttt ttgagtgcca cagcaggttt gatgcttatt
     1381 ggacatattg taagtatagc cagagtgcaa acaggaaatc cagagttaaa tgctttttgg
     1441 ttagtaatga tttttgccgc atttaatact tctggtcgtg tgttgggtgg atttttatct
     1501 gataaaatgg gtcgcacaac aggtttgtta attgtattct tattacaagc aattaatatg
     1561 ttcgttttct cattttatac ttctttctca ttattggttg ttggttttgc cgtggcaggt
     1621 atagcttacg gggctttatt tgcattgttc cccgcagtaa cagccgattt ttttggcgta
     1681 aaaaatttag gggttaatta tggtattatc ttcactagct ggggtattgc aggtatagtg
     1741 ggtccgatat tgggaggtat ggttgctgat ataacaggta cttatgcagg ttcgtatatt
     1801 gtagcaggta ttatgctctt gctaggtgct ttattggtaa aactgataaa aacgccacag
     1861 caggaaatta aataatatta catactgata gtttaaggaa gacctgcatg tagtttgcag
     1921 gtcttttttt tgcatctaca aggtactatt ctgagcttag atatgtatct ttgcataaac
     1981 gaatacaaat aagtaaaata atgaaagcaa ttacacaaaa tagcatagga aatgctgaaa
     2041 caatgtacat aggagaggta gatatacctg tgttaagaga aaatgaagta atggtgcagg
     2101 taaaagcatt tggcgtaaat agggcggata ttttgcaacg aaaagggaaa tatccttctc
     2161 ctgcaggagc cagccctttg atggggttag aagtagcagg aatagttgca gaagcacctg
     2221 tttcgagcaa ctggaaaatt ggagataagg tgtttgggtt gattccagga ggtgggtatg
     2281 ctagttatgt ggctattgat gctgaaatgc tgtggagaat gccagataca atgagttttg
     2341 aagaagcggc agctataccc gaagcttttc ttactgcatt tttagcgtta cagtggcagg
     2401 gaaagttgca acgaggacag aaagttttgc tacacgccgc atcaggaggt gtaggtaccg
     2461 cagctattca attggctaat gccatgcaag ccgaaatact tgtaactgca tcagccagta
     2521 agcatgctat ttgtgctaag ttaggagcaa cggtatgcat tgattataaa acagagtcgt
     2581 gggttgatgt agtacaagaa gtaatgggta aaaatagcat tgacgtggta gtcgattttt
     2641 taggggcttc atattttaac gacaatattt cggtattagc tatggacggc agaatggtaa
     2701 tgctagcatt gatgggtgga gcagacgttt cgggagttga tttacgcaag attattggta
     2761 aacgaattac tgtcattggc tctacattac gtaatcgacc cttatcattt cagagaaaat
     2821 tagcacgtga gtttgctgaa caatttttac catctttttc agatggacgt ttgcaaccca
     2881 taattgatac agtgatggat tggacgctca ttaaagaggc acatgaacga atggagtcga
     2941 atcaacatgt aggaaaaatt gtgatgaaag tatcagagta aataacgccc ttagtatgat
     3001 gtgtaaaaag agttaaaaat agtaacttct tagatggatt gtagtaagta gaatgctcta
     3061 tcaataaata tttacgtaca tttgtataat tttttcttac aatttataag ttaatcgcta
     3121 ctgtgtagca aaaataatat ttatggcaaa agttaataaa gaagatgctt tacgttatca
     3181 cgcagagggc aaaccaggca aaatagaagt tgtaccaact aaaccctata gttcgcaacg
     3241 agatttatca ctagcttatt ccccaggtgt tgctgagccc tgtttagaaa tagaaaaaga
     3301 tgccagtaaa gcgtatgatt ataccgctaa aggcaactta gtagctgtaa tttcaaacgg
     3361 tacagctgtg ctcggattgg gtgatattgg tgccatagct ggaaagcctg ttatggaagg
     3421 taagggcttg ttgtttaaga tttttgcggg tattgatgtg tttgatattg aggtaaacga
     3481 aaaagaccct aaaaaattta tagaagtagt aaaagcgatt gcacctacct tcggaggtat
     3541 caacttagaa gatattaaag ctcccgaatg ttttgagatt gaagatgcat taaagcgtga
     3601 tttggatatt cctattatgc acgatgatca acatggtaca gccatcattt ctgctgctgg
     3661 gttaatcaat gcattggaat taacaaacaa aaatattgag gacgtaaaaa ttgtggttaa
     3721 tggtgcagga gcagcagcaa actcgtgtgc atcattgtat attcagttag gagcaaaact
     3781 cgaaaatatt gtgatgcttg attcaaaagg agttatcaat aaacaacgta cggatttaaa
     3841 tgatagaaaa aagccatttg caacggatag aacgattaaa acattaacag aagctttagc
     3901 aggagctgat gtctttttag gtttatccgt agaaggcgtg ctttcggctg atatgattcg
     3961 tacgat
//
LOCUS       DAOPUR010000086         3944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_23203_length_3944_cov_2.437645, whole genome shotgun sequence.
ACCESSION   DAOPUR010000086 DAOPUR010000000
VERSION     DAOPUR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23203_length_3944_cov_2.437645"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKN90_02210"
     CDS             <1..232
                     /locus_tag="PKN90_02210"
                     /inference="COORDINATES: protein motif:HMM:NF019303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ85124.1"
                     /translation="WIRMFVTLNQQTIFRIKGILCFANLPGKFILQVVGDIVTFESLP
                     DDTEKNTCSKLVFIGTSIPKTDIEISLRNMLA"
     gene            complement(293..589)
                     /locus_tag="PKN90_02215"
     CDS             complement(293..589)
                     /locus_tag="PKN90_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85125.1"
                     /translation="MKKLLLYILLSFAVVGVYATTDKKPVNTVQETQQEVVSVVGDKV
                     YITAKAGEEINIFALTGQNVYRTLSEGGVLVVEDLPTSQILVVRIGAKAYKVKL"
     gene            complement(670..1443)
                     /locus_tag="PKN90_02220"
     CDS             complement(670..1443)
                     /locus_tag="PKN90_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNZ85126.1"
                     /translation="MKILILGAGKMGTFFCDVLCTQHEVAIFDTNPKNLRFVFNTYRL
                     TQYDEIAEFAPELVINAVTLKHTIDAFQAVIAYLPSTCILSDIASVKTGLQEFYASTG
                     MRYVSTHPMFGPTFANLEDLSSQSAIIISESDHMGKAFFKDLYQSLKLQIFEYTFLEH
                     DETIAYSLSIPFASTIVFAAVMKHQDAPGTTFKRHLAIARGLFAEDDYLLSEILFNPF
                     TPSQVEKIRGELKFLLEIIEQKDSKAMHSYLQKLRENIQ"
     gene            complement(1464..2531)
                     /locus_tag="PKN90_02225"
     CDS             complement(1464..2531)
                     /locus_tag="PKN90_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HNZ85127.1"
                     /translation="MNDLQSILLPGIESKRPIVIAGPCSAETEEQVMDTAKQLAKNGI
                     KIYRAGIWKPRTKPGGFEGVGEIGLPWLQKVKEETGMYVSTEVATAKHVEAALKAGID
                     ILWVGARTSANPFAVQEIADSLKGVDIPVLIKNPVNPDLDLWIGAIERIYNAGIRKLG
                     AIHRGFSSYDKKIYRNLPQWHIPIELHRRLPNIPIICDPSHIGGRRELIASISQQAMD
                     LSFDGLIIESHTCPDKAWSDASQQITPDILQFIINKLVIREGVQTTESLADLRLQIDN
                     IDNDLLEMLSKRMRISREIGTFKKEHNMPVLQANRYDEIMQKRVILGESMGMSPEFVT
                     KILEAIHEESVRQQIDVINSK"
     gene            complement(2545..3714)
                     /locus_tag="PKN90_02230"
     CDS             complement(2545..3714)
                     /locus_tag="PKN90_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ85128.1"
                     /translation="MMKIQPANRLNEVSEYYFSRKLKEIAQMNAAGMNVISLGIGSPD
                     MPPSESTIETLAEVAKMPNTHGYQPTIGIPELRKAFADWYVRWYGVQLDANSEIQPLI
                     GSKEGILHVTLAFVNPGEKVLVPNPGYPTYTSLSKLLGAEVVQYDLCEDKGWEPDFEA
                     LQKMDLSNVKLMWTNYPNMPTGANASISLYQKIVDFAKKNNIVVVNDNPYSFILNNKP
                     LSILQIEGAKECCIEFNSLSKSHNMPGWRIGVIAANPTFIEWIVRVKSNIDSGTFRAI
                     QLAAVEALSNTNDWHKEMNIDVYQSRRDIAGKIMNALNCSYDDTQVGMFLWGKVPDNA
                     LSAEEITEKVLHEAKVFITPGFIFGSNGERYIRISLCATQAMLEEALNRIQLIIK"
     gene            complement(3719..>3944)
                     /locus_tag="PKN90_02235"
     CDS             complement(3719..>3944)
                     /locus_tag="PKN90_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNZ85129.1"
                     /translation="HEEGSLAKVLSILSYYHVNLTKIQSLPIIGREWEYMFYINLTFD
                     NYMRYRQALDAIRPFTKDLQLLGEYKAFEG"
BASE COUNT         1201 a          759 c          616 g         1368 t
ORIGIN      
        1 ttggattcgg atgtttgtta cactcaacca acaaactatt tttagaataa aagggatttt
       61 gtgttttgca aatttacctg gaaagtttat actacaggtg gtgggggata ttgttacatt
      121 tgaatcattg ccagatgata ccgaaaaaaa cacatgctca aaacttgtat ttataggcac
      181 atcgataccg aaaacggata tcgaaataag tttacgcaac atgcttgctt aacaaaaaaa
      241 taggagacac aaaattgcat ctcccataaa actaacaaca aatcttttat ctttataatt
      301 tcactttata agctttggct ccaatacgta ctactaaaat ttggcttgta ggtaaatctt
      361 cgaccaccaa cactcctcct tcggaaagag tacgatatac attttgacca gtaagagcaa
      421 atatgtttat ttcttctcct gccttagctg taatatatac cttatcaccc actactgaaa
      481 caacttcttg ttgcgtttct tgcaccgtat ttacgggctt tttgtcggta gtagcataca
      541 ccccaaccac cgcaaaagag agcaatatgt agagtagtaa ttttttcata ggttagtata
      601 tttctgcaaa tatatatgat tttttgagta tatacaaatt ttttataaaa aaaatactta
      661 ctaattcatt tattgaatat tttctcgcaa tttctgcaaa tatgaatgca ttgcttttga
      721 atctttttgt tcaataattt ctagtaaaaa cttcaattcg ccacgaattt tctctacttg
      781 cgaaggtgta aaagggttaa acaaaatttc tgaaagcaaa taatcatctt cggcaaataa
      841 tcctcttgca atggctaagt gtcttttgaa cgttgtacct ggagcatctt gatgtttcat
      901 tactgcagca aatacaatgg tagatgcaaa ggggatagac aacgaatagg caatggtttc
      961 atcatgttct aaaaacgtat attcaaaaat ttgcaatttt aaactttgat ataagtcttt
     1021 aaaaaatgct tttcccatgt gatcggattc ggaaataata atggcacttt gcgaactcaa
     1081 atcttccaaa tttgcaaatg tgggtccaaa cattggatga gtggatacat atcgcatacc
     1141 agtagatgca taaaactcct gcaaacctgt ttttacagac gcgatatccg acaaaataca
     1201 cgtacttggc aaataagcaa ttactgcttg aaatgcatca atggtatgtt tgagtgttac
     1261 cgcattaata actagttctg gggcaaattc cgcaatttca tcatattgtg taaggcggta
     1321 ggtattaaat acaaaacgta aatttttagg attagtatca aaaatagcaa cttcgtgttg
     1381 ggtacataat acatcacaaa agaatgtacc cattttacct gcacctaata tgagaatttt
     1441 catatactta tggatttatt aaattactta gagttaatca catctatttg ttgacgcact
     1501 gattcttcgt gtattgcttc caatatttta gttacaaatt ctggggacat acccatcgac
     1561 tcaccaagaa ttacacgttt ttgcataatc tcatcgtaac gattggcttg taatacaggc
     1621 atattatgct cttttttaaa tgtgcctatc tcacgcgata tacgcatacg tttagataac
     1681 atttctaata aatcattatc aatattgtct atttgcaaac gtaaatcggc caaactttcc
     1741 gttgtttgaa caccctctct aattaccaat ttattgatta taaattgtaa aatatctggc
     1801 gttatttgtt gcgaagcatc gctccatgcc ttatctggac atgtgtgaga ttcgataatt
     1861 aaaccatcaa aacttaaatc catagcttgt tgactaatcg atgcaatcaa ttctcttctt
     1921 cctccaatat ggcttggatc gcaaataatt ggtatattag gcaaacggcg atgtagttcg
     1981 attggaatat gccattgagg taaattacgg tatatttttt tatcatacga actaaatcct
     2041 ctgtggatag ctcctaattt tctaattcct gcattataaa tacgttcaat agcaccaatc
     2101 cataaatcta aatctggatt aacggggttt tttatcaaca caggtatatc cactcctttc
     2161 aatgaatcgg caatttcttg tacagcaaaa ggattagcag aagttcgtgc acctacccag
     2221 agtatatcaa taccagcttt caatgcagct tctacgtgtt tggcagtagc tacttcggta
     2281 gatacataca tacctgtttc ttcttttact ttttgtaacc aaggtaaacc aatttctcct
     2341 acaccttcaa atcctccagg ttttgttcta ggtttccata ttcctgctct gtatattttt
     2401 atcccatttt tcgccaattg tttggctgta tccattactt gctcttctgt ttcggcactg
     2461 caaggtccgg ctattactat cggtcgtttc gactcaatac caggtaataa tattgattgt
     2521 aaatcgttca tgtttttata ttttttattt gattattaat tgaattctat ttaatgcttc
     2581 ttcgagcatg gcttgtgtag cacacaagga aatacgtata tatcgttcgc cattgcttcc
     2641 aaaaataaac ccaggagtaa taaaaacctt ggcttcatgc aatacttttt ctgtaatttc
     2701 ttcagccgat agagcattat caggcacttt tccccataaa aacattccta cttgcgtatc
     2761 atcataacta caattaagag cattcataat ttttccagca atatctctac gcgactgata
     2821 tacatcaata ttcatttctt tatgccaatc gtttgtatta gaaagtgctt ctactgcagc
     2881 taactgtatg gcacgaaatg taccagaatc tatattactt ttcacccgta caatccattc
     2941 gataaatgtt ggatttgcag caatcacacc aatacgccaa ccaggcatat tatgactttt
     3001 gctcaacgaa ttaaattcta tacaacattc ttttgcccct tctatttgta aaatacttag
     3061 cggtttgttg tttaaaataa aactatatgg attatcgttt actactacaa tattattctt
     3121 tttggcaaaa tctacaattt tctggtataa acttatactt gcattggctc ctgttggcat
     3181 atttggataa tttgtccaca tgagttttac atttgataag tccatttttt gtaatgcttc
     3241 aaaatcgggt tcccagcctt tatcttcaca taaatcgtat tgtacaacct ctgcacccaa
     3301 caatttacta agcgaagtat aggtgggata accagggttt ggcactagta ctttttcgcc
     3361 aggattaaca aacgccaaag taacatgcaa aataccttct tttgatccaa ttagcggttg
     3421 tatctcgctg tttgcatcta gttgcactcc ataccaacgt acataccaat cagcaaaggc
     3481 ttttcttaac tctgggatac ctattgttgg ctggtagcca tgggtgttgg gcatttttgc
     3541 tacctcggct aaggtttcaa tcgtgctttc tgatggtggc atatcggggc taccaattcc
     3601 caaacttata acattcatgc ctgccgcatt catttgggct atttctttta acttacgtga
     3661 gaaatagtat tctgatactt catttaatcg attggctggt tgtatcttca tcatattttt
     3721 atccttcaaa agctttatat tctcctaata actgtagatc tttggtaaat ggacgaatag
     3781 catctaatgc ttgtcgataa cgcatgtaat tatcgaatgt taaattgata taaaacatgt
     3841 attcccattc tctcccaata ataggcaatg actgaatttt ggttaagtta acgtgataat
     3901 acgacaaaat ggatagaact ttagctaggc taccttcctc atga
//
LOCUS       DAOPUR010000087         3931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_23316_length_3931_cov_3.216460, whole genome shotgun sequence.
ACCESSION   DAOPUR010000087 DAOPUR010000000
VERSION     DAOPUR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23316_length_3931_cov_3.216460"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PKN90_02240"
     CDS             <1..992
                     /locus_tag="PKN90_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNZ85130.1"
                     /translation="ICTLEFEVHRPLYDWFIDQLATTNYRPQQYEFNRLNITYTVMSK
                     RKMLQLVKEKLVAGWDDPRMPTICGLRRRGYTPSAIRSFINMIGYTKYEGTIDASLME
                     HAIREDLKPKADRVCAILDPIKLIITNYPANQLELLSTQNNPDNEQAGERTIQFGKEL
                     YIERSDYMENAPKDYFRLTEGKEVRLKSAYIVTCTGSKKDENGRVVEVYCTYDAETKS
                     GTEGSKRKVKSTINWVEIGSSIDAEIRLYDRLFAIEDPSKSDQDFRDLLNPDSLHILK
                     GAKVEAYLQNAKPMDSFQFLKHGYFTVDLDSKPNHLIMNRTVSLKDNTPKEKK"
     gene            1082..1660
                     /locus_tag="PKN90_02245"
     CDS             1082..1660
                     /locus_tag="PKN90_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNZ85131.1"
                     /translation="MNKKLFLPISIISIVVILVIWAFSTYNSIVTLDEAVPAQWAQVE
                     NAYKRRADLIPNLVSTVKGYAAHEQGTFEAVVEARAKATQITVDPSNLTPEKLKEYQA
                     AQGQVGSALGKLLMITENYPELKANQNFLELQAQLEGTENRISTERHRFNESTRMFNS
                     KIRRFPGNLFGFDTRPYFEATEAEQAVPTVSF"
     gene            1663..2109
                     /locus_tag="PKN90_02250"
     CDS             1663..2109
                     /locus_tag="PKN90_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNZ85132.1"
                     /translation="MNPKTFFSKADQLRIENAIVEAEKQTSGEIRLHIQSLTKGDVMD
                     AAVGVFYRLEMNKTEQRNGVLFYLSINDHQFAVIGDSGINKVVPSNFWDSIYEGIISY
                     FKQNLYADGLVWAISQAGIQLSSFFPLQKDDTNELSNEISFETKNE"
     gene            2111..2872
                     /locus_tag="PKN90_02255"
     CDS             2111..2872
                     /locus_tag="PKN90_02255"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNZ85133.1"
                     /translation="MHQTVKTLGLMLCMLIFSISASAVIPDKPNPPRLVNDFAGIFAP
                     NELQQLEQYLVLVDDSTSNQIVVVTVNDLGNYDKAQFATELGQQWGVGNASFDNGIVL
                     LIKPKVGNQSGEVFIAVGYGLEAVIPDAIAKRIIEEVTIPYFRQNDYYGGALATVQYL
                     HKLAKGEIDVAREAEPDYTRFVIFLVIFILFIIIASNSKKNNTDGKGGTRHTSYGGAP
                     WIITGGSGGFGRSSGGGFGGFSGGGFGGGGAGGRW"
     gene            2888..3880
                     /locus_tag="PKN90_02260"
     CDS             2888..3880
                     /locus_tag="PKN90_02260"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNZ85134.1"
                     /translation="MQSYPFIDIQDAILRINLWAKQKKAFFFAFDYNLHHAIALLADE
                     IDAENILYKIGGYTNESMQNDLLPANLVWQPQPLSFADYSKKCTYVKEQIQLGNSFLV
                     NLTQPTPIQTNVSLQDLYQLTDAKYKLWVKNHFVVLSPEPFIRIQGLHISSFPMKGTI
                     DASIPNAEKIILENPKEEAEHATIVDLIRNDLSMIATDVHIKRYRYIEHIHSLQKHLL
                     QVSSEICGTLPSNYHENLGTMIRKLLPAGSISGAPKTKTLDIIQSAEGYDRNFYTGIC
                     GYFDGENLDSAVMIRFIEQQDSQLVYKSGGGITANSDIQTEYEELIQKIYVPIR"
BASE COUNT         1372 a          720 c          739 g         1100 t
ORIGIN      
        1 ctatctgcac cttggagttt gaggtacacc gccctttata cgattggttt atcgaccaat
       61 tagcaacaac caattaccgt cctcaacaat atgaatttaa tcgcctaaat attacatata
      121 ccgtaatgag taagcgaaaa atgttacaat tagtaaaaga aaaactagta gcaggatggg
      181 atgacccacg tatgccaact atttgtggct tacgcagaag agggtataca ccaagtgcta
      241 tacgcagctt tatcaatatg attggctata ccaaatacga aggcacaatt gacgcatcat
      301 taatggaaca tgctatacgt gaagatttaa aacctaaagc agatagagta tgtgccattt
      361 tagaccctat taaactcatt attacaaact atccagcaaa ccaactggaa ttattgtcaa
      421 cgcaaaacaa tccagacaat gagcaagcag gtgaacgcac catacaattt ggcaaagaat
      481 tgtatattga acgttctgat tacatggaaa atgcacctaa agattatttc cgtttgaccg
      541 aaggaaaaga agttcgacta aaaagtgcct atattgtaac atgtacaggc tctaaaaaag
      601 atgaaaatgg acgtgttgta gaagtatatt gtacgtatga tgctgaaacc aaaagtggta
      661 cagagggaag taaacgaaaa gtaaaaagca ccataaactg ggttgaaatt ggaagtagca
      721 ttgatgctga aatacgcttg tacgaccgat tatttgccat cgaagatcca tctaaatcag
      781 accaagattt tcgcgatttg ctaaaccccg actccttaca catactaaaa ggagcaaaag
      841 tagaagcata cttacaaaat gcaaaaccca tggatagttt tcaatttttg aaacatggct
      901 attttacagt cgatttagat tcaaagccta atcatctgat aatgaacaga actgtttctt
      961 taaaagacaa tacacctaaa gaaaaaaaat aataacgaat aatgcaccta gtgatatgtt
     1021 cggcattttg aaaaataagt aattttaagt acatttgtat ctgaataaaa tattatcaac
     1081 tatgaacaaa aaactctttc ttccaatttc cattatcagt attgtagtaa tactcgtgat
     1141 atgggcattt agcacgtata atagtattgt aacactcgat gaagctgttc ctgcacaatg
     1201 ggcacaggta gaaaatgcgt acaaacgtcg tgctgattta atacccaacc tagtaagcac
     1261 cgtgaaagga tacgctgcac acgaacaagg cacatttgaa gctgtggtag aagctcgtgc
     1321 taaagcaacg caaattacag ttgacccgag caacttaact cctgagaaac taaaagaata
     1381 ccaagcagct caaggtcaag taggctctgc attaggtaaa ctattgatga ttaccgaaaa
     1441 ttatccagaa ctaaaagcca atcaaaactt tttagaatta caggcacaac ttgaaggaac
     1501 tgaaaataga attagtacag aacgtcatcg tttcaacgaa tcgactcgta tgttcaatag
     1561 caaaatacgt cgtttcccag gaaacttgtt tggatttgat accagaccct attttgaagc
     1621 tactgaagct gaacaggctg taccaactgt tagtttttaa gaatgaatcc aaaaactttt
     1681 ttttcaaaag ctgaccaact acgcattgaa aatgccatag tagaagcaga aaaacaaact
     1741 tcgggagaaa tacgtttgca catccaatca ctcaccaaag gggatgtaat ggatgctgca
     1801 gtcggtgtct tttatcgttt ggagatgaat aaaacagagc aacgcaatgg tgtgttattt
     1861 tatctttcta ttaacgacca tcaatttgct gtaattggag atagcggtat taacaaagta
     1921 gtaccaagca atttttggga cagcatttac gaaggaatta tttcctattt taaacaaaac
     1981 ctgtatgcag atggattggt atgggcaata tcgcaggcag gcattcagct atctagcttt
     2041 ttcccacttc aaaaggatga taccaacgaa ttgagcaacg aaatttcttt tgaaacaaaa
     2101 aatgaataac atgcaccaaa cagtaaaaac tttgggacta atgttatgta tgcttatctt
     2161 ttcgataagt gcatcggctg ttattcccga caaacccaat cctcctcgtt tggtaaacga
     2221 ttttgcagga atttttgctc ccaacgagtt acaacaacta gaacagtatt tggtactggt
     2281 agacgattct acttccaatc aaatagtagt ggttacagta aacgatttgg gcaattacga
     2341 caaagctcag tttgctactg aattagggca acaatggggt gttggaaatg catcatttga
     2401 taatggcatt gtattattaa ttaaacccaa ggtaggcaac caatcgggcg aagtatttat
     2461 tgcagtgggt tatggcttag aagctgttat tcctgatgct attgcaaaac gtattattga
     2521 agaagttact atcccctact ttcgtcaaaa cgactattat ggcggagcat tggctactgt
     2581 tcaatactta cataagctag ctaaaggtga aattgatgta gcaagagaag cagaacctga
     2641 ttacacacgc tttgtaattt ttctagttat atttatccta tttattatta tcgccagcaa
     2701 ttctaaaaaa aataatactg atggcaaagg aggaacacgc cacacaagct atggtggtgc
     2761 tccttggatt ataactggcg gtagtggcgg ttttggtcgt tcgtcaggtg gtggttttgg
     2821 tggattcagt ggtggtggtt ttggtggcgg aggtgccggt ggtcgctggt aacaagtata
     2881 catattgatg caatcttatc catttatcga catacaagat gcaattttac gcatcaattt
     2941 atgggcaaag cagaaaaaag cgttcttttt tgcatttgat tataacctgc atcacgctat
     3001 cgcactttta gcagacgaaa ttgacgcaga aaacatcctt tacaaaattg gtgggtatac
     3061 caacgaatct atgcaaaatg atttgctacc cgcaaatcta gtttggcaac cacaaccact
     3121 ctcatttgca gattacagca agaaatgcac gtatgtaaaa gagcaaattc aattaggcaa
     3181 ttcgtttttg gtaaacttaa cacaaccaac acccattcag acgaatgtat ccttacaaga
     3241 tttgtaccaa ctaacagatg caaaatacaa actttgggta aaaaaccact ttgttgtgtt
     3301 gtctcccgag ccatttattc gcatacaagg attacacatt tcttcattcc ctatgaaagg
     3361 aacaattgat gcttctattc ctaatgcaga aaaaataatt ttagaaaacc ccaaagaaga
     3421 agccgaacac gctactattg ttgatttaat tcgaaatgat ttaagcatga tagccaccga
     3481 tgtacatatc aaacggtatc ggtatattga acacatccat tcattgcaaa aacatctatt
     3541 gcaagtaagt tccgaaatat gtggaacatt gcctagtaac taccacgaaa atttaggcac
     3601 aatgatacga aaactattac ctgcaggttc aattagcggt gctccaaaaa ccaaaacact
     3661 tgacattata caatcagccg agggctacga ccgcaatttt tatacaggta tttgtggcta
     3721 ctttgatgga gaaaatttgg atagtgctgt tatgattcga tttattgaac aacaagacag
     3781 tcaattagta tacaaaagtg gtggtggtat aacagctaat agtgatatac aaactgaata
     3841 cgaagaacta atacaaaaga tttatgtgcc aattcgttga tgccatacaa tatagtaatg
     3901 gtgagtttaa acgaatagcc taccaccaaa a
//
LOCUS       DAOPUR010000088         3906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_23514_length_3906_cov_3.158660, whole genome shotgun sequence.
ACCESSION   DAOPUR010000088 DAOPUR010000000
VERSION     DAOPUR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23514_length_3906_cov_3.158660"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PKN90_02265"
     CDS             <1..1141
                     /locus_tag="PKN90_02265"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNZ85135.1"
                     /translation="KIKIGDTLWAGNVEIHKKASDWNAHKHPTNPAYNSVILHVVAEC
                     NTDIFTQEGRKIPQLKLPLTPALLEQWQQLEHSPHKIACHLHLHRISDMIKTSWLEAL
                     VIERLETKASRIDSLLEQNKNNWEETFYQTLARNFGFGLNSDPFEWLAKSLPINYIAK
                     HKNQLLQIEALLFGQSGLLQLSQATDDYTVQLKNEYAFLQQKFSLTPIDGSLFKMMRL
                     RPANFPHIRLAQLAQLIHTSSKLFSHIVENPTYSSLKKMFAVSTSPYWETHYTFGEVS
                     PRKTKKLGEKAINSIIINTIVPILFAYAKRKNNDELQEKSVHLLESCAAEDNSIVRNW
                     QEMGFTATSAYQTQALIQLQKQYCQTKKCLYCRFGHKLLQLSLQP"
     gene            1138..2940
                     /locus_tag="PKN90_02270"
     CDS             1138..2940
                     /locus_tag="PKN90_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNZ85136.1"
                     /translation="MISTLIKRILLISAITVSVTLLLHSCANRGAGPQGGIRDTTPPQ
                     VLKSSQSNKATSATNQRLEWIFDENIQLEKQQENILVSPPQKQAPTIRAMGKKLSVAF
                     ADSLASNTTYTIDFGNAVVDLNEKNPLANFSWSFATGATIDTMQLAGYVIDARTLTPY
                     EGIIVGVYKSNKDSAFVSTPFDYVARTNSQGFFTIRNIPQVPYQVYALKDIPQAFYYD
                     QKGKEIGFFSQKVTPEVVKHLQTDTIWNDNDTVSILRIDTSYSISYAPNDVLIKLFRE
                     ERAMIPTLRRALRPSKESFTLLMSAPLDKEPLITPINVETSKWLKREQTNRMDSLVYW
                     ITDSNALTLDTIQFTVSYASADSLKTDTIALTYKQPRARTANEKTLQQLEIVSLTKSP
                     VDIADTLRLQVKEPMKSIDIEKMHLTMRIGTTWHNVPFLLQYDDDNCKKKISVLFDRK
                     ERANYRLVIDSAAIVSDYGKQNASFVQTFTTKSPDDYASLMIEWKNIPPHGIVELLDA
                     KETVVRAVHVSEPTTVFRLLSPGNYFLRLVLDANQNGKWDTGSVLPKQEPEEVYYYPK
                     RLVLRANWDSVEEWDYTAIPLLQQRATELPKSKK"
     gene            2944..3783
                     /locus_tag="PKN90_02275"
     CDS             2944..3783
                     /locus_tag="PKN90_02275"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNZ85137.1"
                     /translation="MKKLFVYILLFVLPIGNIHSQETNSCGITNTSFKAGEKLRYSAY
                     FRWGILQLSSFSIQSEVKRERYKGESVFAITTTGVTAGNMDKFFTIRDTLHAKLKVND
                     LQPLYFKEIANEEKFKATREFKYDYTDSTRVSWQFADNKNKEKAGNTTLTNCAYDAVS
                     IIYRLRNYDFNAETLKPNQTFRIDYFSDGKSSPLQMRFEKKEIVTLKNGDRYDCLKFE
                     LEVAEGTLFKNNERVFIWITNDKNRLIAYVEAKLKIGYVKMDLVNVEHHRHPLAAKLK
                     PVK"
BASE COUNT         1370 a          837 c          722 g          977 t
ORIGIN      
        1 aaaaattaag attggcgata ccctttgggc gggcaatgta gaaattcata aaaaagcgtc
       61 cgattggaat gcacacaaac acccaactaa tcccgcatat aattctgtta tactgcatgt
      121 agttgcagaa tgtaatacag atatttttac acaagaaggt agaaaaatac cccaactaaa
      181 actgccactt acccctgctc tgttggagca atggcaacag ctggaacaca gtccgcacaa
      241 aatagcttgt catttgcacc tacatcgcat cagtgatatg ataaaaacta gttggctaga
      301 ggctctcgtt atagagcgtt tggaaaccaa agcatcacgc attgacagct tgctagaaca
      361 aaacaaaaac aattgggaag agacttttta tcaaacactt gcacgcaatt ttggatttgg
      421 attaaacagc gacccatttg aatggttagc taaatcgtta ccaataaact acatagcaaa
      481 acacaaaaac caacttttac aaatagaagc cttgcttttt ggacaatcgg gtttattaca
      541 gttaagccaa gccacagatg attatactgt gcaattgaag aatgaatatg cctttttgca
      601 acaaaaattt tcgcttacac ctatcgatgg cagtttattt aagatgatgc gattgcgacc
      661 agctaatttt ccgcacattc ggttggctca acttgcacaa ttgatacaca catcgtcaaa
      721 acttttttcg cacatagtag aaaaccctac ttatagcagc ctaaaaaaga tgtttgcggt
      781 aagcacatca ccgtattggg aaacacatta tacctttggc gaagtttcgc cacgaaaaac
      841 aaaaaagcta ggagaaaaag ccatcaacag catcatcatc aacaccattg tccccatatt
      901 atttgcgtat gcaaaaagga aaaataatga tgaattacaa gaaaaatccg tacatttgct
      961 cgaaagttgt gctgcagaag acaatagtat tgtgcgaaat tggcaagaaa tgggttttac
     1021 agccacttca gcctatcaaa cacaagctct tatacagcta caaaaacagt attgtcagac
     1081 aaaaaaatgt ttgtattgtc gatttggaca taaattatta caattaagtt tacaaccatg
     1141 atatcaaccc tgattaaacg cattttactc atttcagcca tcacagtaag tgtaaccttg
     1201 ctattgcatt cgtgtgccaa tagaggtgct ggaccacaag gtggcatacg cgacacaaca
     1261 ccgccacaag tgctaaaaag tagccagtca aacaaagcta cttcggcaac aaaccaacga
     1321 ttagaatgga tttttgacga aaatattcaa cttgaaaaac aacaagaaaa tatccttgtt
     1381 tcacctccac aaaaacaagc cccaacgata cgagcaatgg gtaaaaaact gagcgttgct
     1441 ttcgctgaca gtcttgcttc aaacactacc tataccattg attttgggaa tgctgtggtc
     1501 gatttgaacg aaaaaaatcc acttgccaac ttctcgtgga gttttgccac aggagcaacc
     1561 attgatacca tgcaactcgc tggttatgta atagatgcac gcacgcttac tccatacgaa
     1621 ggcatcattg taggggtata taaaagcaat aaggatagtg cgtttgtttc tactccgttt
     1681 gattatgtgg ctcgcaccaa tagccaaggt ttttttacaa tccgaaacat tccccaagta
     1741 ccttatcaag tatatgccct aaaagatata ccacaggcat tttattacga ccaaaaagga
     1801 aaagaaatag gttttttcag ccaaaaagtt acccccgaag tggtaaaaca cttgcaaaca
     1861 gatactattt ggaacgacaa cgatactgtt tctatcttgc gaatcgacac gtcttactct
     1921 atttcgtatg cgcccaatga tgtgcttatc aaactctttc gcgaggagcg agcaatgatt
     1981 cctactttac gaagggctct ccgccccagc aaagagtcgt ttactctact tatgtccgca
     2041 cctctcgaca aagaaccgct gattacccct atcaacgtag aaaccagcaa gtggttgaaa
     2101 cgcgaacaga caaaccgcat ggactctctg gtgtattgga ttactgactc caatgcctta
     2161 acgctggata ctatccaatt tacagtatcg tatgccagtg cagatagcct aaaaacggac
     2221 acaatagcat taacatacaa acagccacga gcaagaactg ctaacgagaa aaccctacaa
     2281 cagcttgaaa ttgtatcgct aacaaaatct ccagtcgata ttgcagacac tctccgtttg
     2341 caggtaaaag aaccgatgaa atcgattgat attgaaaaaa tgcatctcac catgcgtata
     2401 ggtactacgt ggcacaatgt tcctttttta ttgcaatatg acgacgataa ctgcaagaaa
     2461 aaaatcagtg tattgttcga ccgaaaggaa agagcgaact atcgcttggt aatagactcg
     2521 gcagctatcg taagtgatta tggcaaacaa aacgcctcgt ttgtacaaac atttacaacc
     2581 aaatcgcccg atgattacgc ttctttaatg attgagtgga agaatatacc acctcatggc
     2641 atagtggagt tgctagatgc caaagaaaca gttgtacgtg cagtacacgt aagcgaacca
     2701 acaaccgtat ttcgtcttct tagtccaggt aactattttt tgcgtttggt actcgatgct
     2761 aatcaaaacg ggaaatggga tacgggaagt gtattgccca agcaagaacc cgaagaagtg
     2821 tattactacc ctaagcgttt ggtattgcga gctaactggg acagtgtaga agaatgggat
     2881 tataccgcca tacccttgtt gcaacaacga gctactgaat taccaaaaag caaaaaataa
     2941 gccatgaaga aactattcgt atatatactg ctattcgttt tgcctattgg gaatatacac
     3001 tcccaagaaa caaactcgtg tgggataact aatacaagtt ttaaagcagg cgaaaagcta
     3061 aggtatagtg cctattttcg ttggggaata ctacaattga gcagttttag catccaaagc
     3121 gaagtaaaaa gagaacgata taaaggagaa tcggtatttg ccattaccac aacgggcgta
     3181 acagcgggca atatggataa attttttact atacgcgata ccttacatgc caaattaaaa
     3241 gtaaacgacc tacaaccgct ttattttaaa gaaattgcaa acgaagaaaa atttaaagcc
     3301 acacgagaat ttaagtatga ttacaccgac tcaacgcggg taagctggca atttgctgac
     3361 aataaaaaca aggaaaaagc aggaaacaca acgctaacaa attgtgcata tgacgcagta
     3421 agcattattt atcgcttacg caattatgat ttcaatgcag aaacgctcaa accaaaccaa
     3481 acatttcgta tcgactattt tagcgatggc aaatcttcgc ccttgcaaat gcgttttgag
     3541 aaaaaagaaa tagttaccct taaaaacggt gatagatacg actgcctaaa atttgaacta
     3601 gaagtagccg aaggcaccct cttcaaaaac aacgaacgcg tatttatctg gattaccaac
     3661 gacaaaaaca gactcatcgc ctacgtagaa gccaaactaa aaatcggcta cgtaaaaatg
     3721 gacttggtaa acgtagaaca ccaccgacac ccattagctg ctaagttgaa gcctgtgaaa
     3781 taggcatagc ctgcccattg caaaggaaat tacgcaaagc gaatcgcact gacacggata
     3841 ccgtttgcta gtataaaaac caaaacatga cctataaccc tgccaaacat aaccgccaca
     3901 gtatac
//
LOCUS       DAOPUR010000089         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_23520_length_3905_cov_2.740779, whole genome shotgun sequence.
ACCESSION   DAOPUR010000089 DAOPUR010000000
VERSION     DAOPUR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23520_length_3905_cov_2.740779"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKN90_02280"
     CDS             <1..280
                     /locus_tag="PKN90_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNZ85138.1"
                     /translation="LKDFGDKLPADKKAPIEAALNKLKEAHKTQNLDGIDAATKELND
                     VFQAASQEMYNAQAQQGGNAQPNAEPQANNQGNDGKDTVTDVDFEEVK"
     gene            506..1177
                     /locus_tag="PKN90_02285"
     CDS             506..1177
                     /locus_tag="PKN90_02285"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ85139.1"
                     /translation="MKKILFLTSVLVVWQLSLTAQNFYEFKELPYSYSALEPYIDSTT
                     METHYAKHHKGYYNNFVKAMQEGNFDNKPLEQVIQNISSYSVFIRNNGGGYWNHEFFW
                     NCMSPNKTQASNALTKAITDNFGSFENFKKEFSSAASTVFGSGWAWLIVTDSGKLKIV
                     KTANQDNPLMDVVSDKGTPILAIDVWEHAYYLKHKNLRAAYVENFWQVINWQFVSEKY
                     ADAVK"
     gene            complement(1266..2768)
                     /locus_tag="PKN90_02290"
     CDS             complement(1266..2768)
                     /locus_tag="PKN90_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85140.1"
                     /translation="MKTKKTFFKVFGVAIAVALLGASCTNVEPTPKPTKPTEPGIGFS
                     LCDDLSDPIISLSENFDFDDVANNSDISLTGWQVFKVKGDNNWQGKIFTSGTNTEKYA
                     QASAHKGGEANYESWLVSPPLNLDDADSKSLSFKTAKAFWKETSSLKVYVLQCNGSTT
                     TKTELTSAYIAKETDTDHVFVSSGIVDLSSFSGKVYIGFQYIALGGASNSTTFRIDDV
                     YFNNTSSTETQVSFVSQAPTSAYIDTEFAYNIQTNVINALGDTEISATGLPAWASFAA
                     NTNGTASITGTPDEAGSYSITITATNNSVVATQVFTLVVTPAPVVGDNLLTNPSFELW
                     EENKPKGWSLIITTSTPVTGAANSEESTIVQDGDKSFKVDASGSSGTVNWAQTVAITG
                     GQKYRLSMSYYILSGDGTDARIWSNFKKGDTFFEEADLIATNLYSVLRGPGNTNSSGS
                     AYLPDEKGAWKTYTITFTAPTDATAFNFQFRTYRNAVVYWDNFSLVNVIE"
     gene            complement(2794..3720)
                     /locus_tag="PKN90_02295"
     CDS             complement(2794..3720)
                     /locus_tag="PKN90_02295"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HNZ85141.1"
                     /translation="MKVTHSILVVGLAWLLQSCYPNFTDMPLEKDQVKRERVPATYTI
                     AQVKNEFLTSPTNRFSAYQIKSNEPVVIEGIITSDDNAGNIYKYIVVQEEGDNPQAIR
                     ISIDAGSMSGIYPVGQRVSVTLNDMWIGSYGQSPQIGTYYYNVEKSREEPGRLAKVLA
                     DQKLVPYGLPDPKAIVPKEMTIAEIRASGSELYHQLVIIKNATFTGKGKDNKTSLPAT
                     ITDAEKIFAPPTNGVGYPQSREITDGTGSIFIATSEYARFANKKLPTGTGDIVALVGW
                     YNDKDENIDPTKIYHQLTIRSLSDLTGFTFPE"
BASE COUNT         1293 a          745 c          641 g         1226 t
ORIGIN      
        1 gctaaaagac tttggagata aattgcctgc tgataaaaaa gcacctatcg aagcagctct
       61 taataaactt aaagaagctc acaaaaccca aaacttagac ggtattgatg cagcgacaaa
      121 agaacttaac gacgtgttcc aagctgctag ccaagaaatg tacaatgctc aagcacaaca
      181 agggggaaat gcacaaccta acgctgagcc tcaagctaac aaccaaggta acgatggcaa
      241 agacactgtt actgatgttg actttgaaga agtaaagtaa gctaaacttt catcctaaaa
      301 aagctgtaaa taaaaattta tttacagctt tttttatatc taccaccaaa gaaaataatg
      361 aattgaaaaa tcccacaagc acctttttct ctacgattaa taagaaactc ttctgtttat
      421 attcattgca tatcactaaa acatgttaac taaaatcgca aaagggcatt ttgggtttat
      481 atttgcaaaa ttaaacaata aaaatatgaa aaaaatacta ttccttacga gtgtactagt
      541 tgtatggcaa ctatcactta cagctcaaaa tttctatgaa tttaaagaac taccctattc
      601 gtatagtgca ttagaaccct atatcgactc caccaccatg gaaacgcatt atgctaaaca
      661 ccacaaagga tactacaaca actttgtaaa agcgatgcaa gagggcaatt ttgacaataa
      721 acctctcgaa caagtaatac aaaacatttc ctcttacagt gtatttattc gtaacaacgg
      781 agggggatat tggaatcatg agtttttctg gaattgtatg tcgccaaaca aaacacaagc
      841 cagcaatgca ctcacaaaag ccattacaga caatttcggt tcgtttgaga atttcaaaaa
      901 agaattctct tcggcagcat ctaccgtttt tggttcggga tgggcatggc ttattgtaac
      961 agatagcggc aaactgaaaa ttgtaaaaac cgctaaccaa gacaatcctt tgatggatgt
     1021 tgtttcagat aaaggaacac ccatcttagc cattgatgta tgggagcacg cctattattt
     1081 gaaacataaa aacctacgtg cagcgtatgt tgaaaatttc tggcaagtta tcaattggca
     1141 atttgtatca gaaaaatatg cggatgcagt aaaataaaat agcaataaat acttaataac
     1201 gaagagggta aatcaatgat ttaccctctt cgttatataa tcgtattcat taaaacgcta
     1261 caaatttatt caattacatt tacgagagag aaattatccc aatatactac agcattacga
     1321 tatgttctaa attgaaaatt gaatgcagtg gcatctgtag gagcggtaaa tgtaattgtg
     1381 tatgttttcc aagcaccctt ttcatctggt aaataggcag atccagacga atttgtattg
     1441 ccaggtcctc taagaacaga ataaagattg gtagcaatta aatctgcttc ttcaaagaaa
     1501 gtatctccct ttttaaaatt actccaaata cgtgcatcag tgccatctcc tgacaaaata
     1561 tagtacgaca tggataagcg atatttttgc cctcctgtta tagcaactgt ttgagcccaa
     1621 ttaacagtac cagaagagcc actagcatca actttaaaag atttatcacc atcttgtaca
     1681 attgttgatt cttcagaatt tgctgcacca gttacaggag tactagtagt aattattaat
     1741 gaccatccct taggcttatt ttcttcccac aattcaaagc ttggattagt aagcaaattg
     1801 tcacctacta caggagcagg agttaccaca agtgtaaata cttgagtagc cactactgaa
     1861 ttatttgtag cagtgatagt gattgaataa gaacctgctt catctggagt acctgtaata
     1921 cttgctgtgc cattagtatt tgctgcgaag cttgcccaag ctggcaatcc agttgccgaa
     1981 atttctgtgt cgcctaatgc atttattaca tttgtttgta tattatatgc aaattcagta
     2041 tcaatataag cagaagttgg tgcctgtgaa acaaaagaaa cctgagtttc tgtactagat
     2101 gtattgttaa agtatacatc atcaattcta aatgttgttg agttacttgc accaccaaga
     2161 gctatatatt ggaatccaat atatacttta ccactaaatg atgagagatc aacaatacct
     2221 gaagaaacaa aaacatgatc ggtatcagtt tcttttgcaa tgtatgcaga agttaattct
     2281 gttttagtgg tagttgaacc attacattgc aatacatata cttttagact agaagtttct
     2341 ttccaaaaag ctttggctgt tttaaatgat aaagacttag aatcagcgtc atcaagattt
     2401 aatggtggag atactaacca actctcgtaa tttgcttctc ctcccttatg tgctgatgct
     2461 tgtgcatatt tttcagtatt tgttccactt gtaaaaattt ttccttgcca attgttatct
     2521 ccttttactt taaacacttg ccaaccagtt aatgatatat ctgaattatt cgctacatca
     2581 tcaaaatcaa aattttcaga cagactaata attgggtcac tgagatcatc acataaacta
     2641 aaaccaatac ctggttccgt tggtttagtt ggcttgggag ttggttctac attcgtacac
     2701 gatgcaccaa ggagtgcaac agcaatagct actccaaaga ctttaaaaaa tgtttttttt
     2761 gttttcatat ctatcctatt tttaaattat tatttattca ggaaaagtaa acccagttaa
     2821 atcactcaac gaacgtatgg ttaattgatg gtaaattttt gttgggtcta tgttttcatc
     2881 tttgtcatta taccaaccca ctaaggcaac aatatcaccc gtacctgttg gtaatttttt
     2941 attagcaaac cgagcatatt cgctggtagc aataaaaata gaacccgttc catcggttat
     3001 ttcccgagat tgcggataac caactccatt agtaggtggg gcaaaaatct tttcagcatc
     3061 agttatagta gcaggtaaac tagttttgtt atctttacct tttccagtaa acgtagcatt
     3121 tttaataatc actaactgat gataaagctc tgaacctgat gctctaatct cggcaatggt
     3181 catttctttg ggtacaatgg ctttagggtc aggcaatcca tatggcacaa gtttttgatc
     3241 agctaatact ttggctaacc tacctggctc ttcacgagat ttctctacat tataataata
     3301 cgtacctatt tggggtgatt gtccatagga gcctatccac atatcattaa gagttaccga
     3361 tactcgttga cccacaggat atattccact catactgcct gcatctatgg agatacggat
     3421 agcctgcgga ttatcgcctt cttcttgcac aacaatgtat ttgtaaatat tccctgcatt
     3481 atcatccgac gtaataatac cctctatcac tacaggttcg ttacttttga tttggtacgc
     3541 agaaaaacgg ttagttggac tagttaaaaa ttcatttttc acttgagcaa tggtatacgt
     3601 agctggtact cgctcgcgtt ttacttgatc tttctccaaa ggcatatctg taaaatttgg
     3661 ataacaggat tgcaataacc atgctaaacc tacaaccaat atactatgtg ttactttcat
     3721 atgtatctta ttttaaaata attgtcaatt aaaaacggta tcctacatgc acaaagaaat
     3781 taaatcctaa agcataataa tatttatttg caaataaatc taactttgtt ttatctataa
     3841 cgccatcgtc caaaggcaaa cgagcttgtt gatatccccc cgttatcatc ttggtattgt
     3901 tcaac
//
LOCUS       DAOPUR010000090         3891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_23640_length_3891_cov_2.935349, whole genome shotgun sequence.
ACCESSION   DAOPUR010000090 DAOPUR010000000
VERSION     DAOPUR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23640_length_3891_cov_2.935349"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PKN90_02300"
     CDS             <1..498
                     /locus_tag="PKN90_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNZ85142.1"
                     /translation="QIRTDDIVVINGTTGVVEKIELRTICLRDHSGVVHIFQHGKVNT
                     LSNMTKEWSALVFEIGVAYKEDVDTVIAIMKSVGEDLEKDSRFSGNILEPISILGVEQ
                     LANSSIQLKVRIKTQTGEQWQTRREYMRRLKYAFDKNGIEIPFPHTTLYWGEKSKPMY
                     ISLQK"
     gene            complement(487..1023)
                     /locus_tag="PKN90_02305"
     CDS             complement(487..1023)
                     /locus_tag="PKN90_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNZ85143.1"
                     /translation="MNPYVILQKYYSSNEEAYDILVNHSESVANKALSIAHNHPELKI
                     DTEFVFQAAMLHDIGIHLTNAPSIACYGSYPYVCHGYLGSGILMEEQLPQHALVCERH
                     TGTGISLEEIVTRKLPLPLRDMKPISLEEQLICFADLFFSKTHLEEELSITRIETKLQ
                     KFSSHSVVQFHQWCDLFL"
     gene            complement(1023..2639)
                     /locus_tag="PKN90_02310"
     CDS             complement(1023..2639)
                     /locus_tag="PKN90_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ85144.1"
                     /translation="MEKHKLLYPIIIGISIAVGILLANLYYQQAEQTTGFAIPKKVQG
                     NKFDEIMYLLDNNYVDVVDKNALTEEFIPQILKFLDPHSAYLTPEEVAESATELQGSF
                     SGIGVQFNIQNDTVMIVSVIPGGPSEKLGISPGDRIIAVNDSSFVGADVTNATVLKTL
                     RGEKGSIVKLRIKRNGSPLPLDFDIVRGDVPVKSIDVVYVIADSTGYVKISNFGANTY
                     REFLDALVILREQECNNIIVDLRNNSGGYLSAVIQMTNEFLKRGQLIVYTEGVNQARE
                     DAIADGRGNFRKENICVLINEWSASASEIFAGAIQDHDRGWVIGRRSFGKGLVQQQIP
                     LSDGSELRLTVARYHTPSGRSIQKPYKSGESDENYDVEIEKRVLHGEMFQKDSISFAD
                     SLKYYTAKGRTVYANGGIMPDIFVPHDTTYYTPYYTNVLNNGIVYDFSFAFVDKHRAK
                     LSTYDTWEKLVEYLVKKTTYFSEFIRYAEQKGVSRNSQEIAKSKALLANLLQAYIARN
                     LLGDEAFYPILNLQDNTVKKAIEMVGKPIQ"
     gene            complement(2643..3068)
                     /locus_tag="PKN90_02315"
     CDS             complement(2643..3068)
                     /locus_tag="PKN90_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNZ85145.1"
                     /translation="MNTKQLVLDKRYMQMALIWAENSYCKRRQVGAILVKDKMIISDG
                     YNGTPCGFENICEDESFKTKPYVLHAEANAITKIARSHNSSEGATLYVTTSPCIECAK
                     LIIQSGIVRVVYANEYHVTDGVDLLVRAGVEVLKIDLDN"
     gene            complement(3107..>3891)
                     /locus_tag="PKN90_02320"
     CDS             complement(3107..>3891)
                     /locus_tag="PKN90_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNZ85146.1"
                     /translation="SIVHYVAQTQRKMTASQDKYQRGKIVAQDFTEETTQLVRQLKDF
                     LRKEVYQDTRSSVAYFALYQQINGYYVFNPYDKEDVKVYAAVATPLQVHYPQTARTKN
                     IEDVVLKGMQRRKNAEDKLVREIVGKGFIDISLSTVQGVERSLSASLGRATIVDFSLF
                     QSENSYAHTVLLQELYRVYASKGLRIYQVSFDDDAVFSVVAKQLPYTVVHDKKGLVSP
                     LLQTYNISTLPTVFLLDKEGDIVARNVSLPLLEQSIQQLLEK"
BASE COUNT         1243 a          789 c          618 g         1241 t
ORIGIN      
        1 caaattagaa cagacgacat cgtagtaatt aatggaacaa ctggggtagt ggaaaaaatt
       61 gaattacgta caatttgttt acgcgaccat tctggtgtag ttcatatttt tcaacatgga
      121 aaagtcaata ccttatctaa tatgaccaaa gaatggtcgg cattagtatt tgaaatcggt
      181 gtagcataca aagaagatgt ggatacggta atagctatta tgaaatcagt aggtgaagat
      241 ttagaaaaag attcaagatt ttcaggaaat atattagagc ctatctctat acttggagta
      301 gaacaattag ccaacagttc tattcaactc aaagtgcgta tcaaaaccca aacaggagag
      361 caatggcaaa ctagaagaga atatatgcgt cgattaaaat atgcatttga taaaaacggt
      421 attgaaattc ctttccctca taccacactg tactggggcg aaaaaagcaa acccatgtat
      481 atttcactac aaaaataaat cgcaccactg gtggaactgc acaacagaat gtgaactaaa
      541 tttttgcaat ttcgtttcaa tccttgtgat agataattct tcttctagat gtgttttcga
      601 aaaaaataaa tcggcaaaac aaattaattg ctcttctaac gatataggtt tcatgtctcg
      661 taatgggaga ggcaacttgc gagttacaat ctcttccaaa ctaatacctg tacctgtatg
      721 gcgttcgcaa actagggcat gctgaggcaa ttgttcttcc atcaagatgc cagaccctaa
      781 atagccatga caaacatatg gataagaacc ataacaagca atacttgggg cattggttaa
      841 atgtatccca atatcgtgca acatagctgc ctgaaaaaca aattctgtat ctatcttcaa
      901 ttcaggatga ttgtgagcaa tagaaagggc tttattagca acactctcgc tatgatttac
      961 caatatgtca tacgcttctt catttgaaga ataatatttt tgtaaaatta catacggatt
     1021 cattattgta ttggtttacc aaccatctca atggcttttt tcacagtatt atcttgtaaa
     1081 ttaagtattg gataaaatgc ttcatcacca agcaaattac gagcaatata tgcttgtagt
     1141 aaatttgcta agagagcttt cgatttcgca atctcttgac tattgcggga aacacctttt
     1201 tgttctgcat aacgaataaa ttcgctaaaa taagttgttt tctttactaa gtattctact
     1261 aatttttccc acgtgtcata agtagataat ttagctcgat gcttatcaac aaaagcaaac
     1321 gaaaaatcat atacaatgcc attatttaaa acgtttgtgt aatatggtgt ataataggta
     1381 gtatcatgag ggacaaaaat gtcgggcata atacctccat tagcatatac agttcttcct
     1441 ttggcagtat aatacttcaa actatcagca aaagaaatac tatctttttg gaacatttca
     1501 ccatgtaata ctcgtttttc tatttctaca tcataatttt catccgactc gcctgacttg
     1561 tagggtttct gaatagaacg acctgatggt gtgtgatagc gagccacagt aagccttaat
     1621 tcagagccat cggacaacgg aatttgttgt tgcactaaac ccttaccaaa agagcgtctg
     1681 ccaattaccc atcccctatc atgatcttga atggctccag caaatatttc gctagcagaa
     1741 gcagaccatt cgtttatcaa cacacagata ttctcttttc ggaaattacc tcttccatct
     1801 gcaatggcat cttctctggc ttgattaact ccctctgtat atacaattaa ttgacctctt
     1861 tttaaaaatt cattcgtcat ctgaatgacc gcggataaat acccccctga attgttgcgt
     1921 aaatcaacaa taatattatt gcattcttgt tcacgtaata tgactaatgc atctaaaaat
     1981 tcacggtatg tattagcccc aaaattacta attttgacat atccagtaga atcggctatt
     2041 acgtatacca catctatact ttttactggt acatctcctc tcacaatatc aaaatcaaga
     2101 ggcaaggggc ttccgttgcg tttaatccgt agtttaacaa tactgccctt ttctcctctc
     2161 aatgttttta gcactgtggc attggtaaca tcagctccta caaaactact atcattcact
     2221 gcaataatac gatcacctgg cgatattccc aatttttcag aaggaccacc aggaatcaca
     2281 ctcacaatca taaccgtatc attctgaata ttaaattgca ccccaatacc actaaaacta
     2341 ccttgtaact cagtagctga ttctgctact tcttctggag ttaaataagc tgaatgaggg
     2401 tcgagaaatt tcagtatctg tggtataaac tcttctgtta aagcattttt atctacaaca
     2461 tccacataat tattgtctaa caaatacata atttcatcaa acttattccc ttgaaccttt
     2521 ttaggaatag caaaacctgt agtttgttct gcctgttgat aatacaaatt tgctaacaat
     2581 attccaaccg caatggatat accaataatg attggataaa gcaatttatg tttttccata
     2641 cgttaattat ctaaatcaat tttgagcact tctaccccgg ctctcacgag caaatcaacc
     2701 ccatcagtaa catggtattc attggcatac acaaccctca caatacccga ttgaataatc
     2761 aatttggcac attctatgca tggagatgtc gttacatata acgtagctcc ttcgctacta
     2821 ttgtgagaac gtgcaatttt tgtaatcgca tttgcctctg catgaagcac atagggtttg
     2881 gttttaaaac tttcgtcttc acaaatattt tcaaaaccac acggcgtacc attgtaacca
     2941 tcagaaataa tcattttatc ttttactaat attgctccta cctgacggcg tttgcaatac
     3001 gagttttcag cccaaataag agccatttgc atatatcttt tgtctaatac taactgtttt
     3061 gtattcatat ctcactatct aaaacgaatt gatactgttc gctgttttat ttttccagta
     3121 attgttgaat ggattgttcc aataaaggta acgaaacatt gcgtgccact atatctcctt
     3181 ccttatccaa caaaaataca gtgggtaagg ttgatatatt atacgtttgc aacaaaggag
     3241 atactaatcc tttcttatca tgaacaactg tatagggcaa ctgtttggca acaacagaaa
     3301 atacagcatc atcgtcaaac gaaacctgat aaatgcgtaa tcctttgctg gcatatacac
     3361 ggtaaagttc ttgtagcaat acagtatgtg catatgaatt ttcggattga aacaacgaaa
     3421 aatccactat agtggcacgc cctaaggatg ctgataaaga acgctctacc ccttgcaccg
     3481 tggacaagga aatatcaata aaacctttgc caactatttc tctaactagt ttatcttctg
     3541 cattttttcg tcgttgcata ccttttagca ccacgtcttc gatattcttt gtacgtgccg
     3601 tttgtggata atgcacttgc aagggtgtag ccactgcagc atacactttt acatcttctt
     3661 tatcataagg attaaacaca taatacccat taatctgctg atacaaagcg aaataagcca
     3721 cacttgatcg cgtatcttga tatacctctt tgcgtaagaa atcttttagt tgacgaacaa
     3781 gctgcgtcgt ttcttctgta aaatcttgtg ccactatttt gccacgttga tacttatctt
     3841 gactagctgt catcttacgt tgtgtctgtg caacatagtg cacaatggat t
//
LOCUS       DAOPUR010000091         3890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_23645_length_3890_cov_3.888396, whole genome shotgun sequence.
ACCESSION   DAOPUR010000091 DAOPUR010000000
VERSION     DAOPUR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23645_length_3890_cov_3.888396"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PKN90_02325"
     CDS             <1..808
                     /locus_tag="PKN90_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ85147.1"
                     /translation="LKSKLHNTTCKVSRFKEQIKKTKPHKHDNYYELIILLEGSGFHQ
                     IESTHYLIQAPVCFVLQPGQMHYWQFTSIPKGYVILYSNDEFDDLHEKNVCLLYKQLA
                     FHTHINISKETLPIALLEELYKVYSEPNHFSQLIIHGLLQSLFATLLITTQNHDLQAQ
                     SMSLPFEQFSKLIQQHIHTHHKVYQYAALLNMTPQNLNAICLKHGKTNAANHINNHLL
                     LEAKRYILHTEKTIAEIADLLSFSDSSHFVKFFKKYEGITPVQFRQQFFQ"
     gene            1079..1630
                     /locus_tag="PKN90_02330"
     CDS             1079..1630
                     /locus_tag="PKN90_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNZ85148.1"
                     /translation="MKRIGERIKKYRRIYNMALGDLATKVGITPSALSQIENAKAFPS
                     IITLKSIAENLHTTVGELIGENEASKHSPVYRAVDYKLLDNLDYKATIYHIPYEDVNK
                     QMDSFVIILKPQEKVENIFASHSGQRMCYVLQGNIEFTIDEKKVELKTGDSAFFHAKN
                     ISILQNKQQEDTKLLLVIAPASM"
     gene            1698..2288
                     /locus_tag="PKN90_02335"
     CDS             1698..2288
                     /locus_tag="PKN90_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ85149.1"
                     /translation="MCNREQKITKEKQKFPAIFLDRDGTLIEDVGYIKEPSAVSFFPN
                     TFADLIRLQEHFMLFIITNQSGIGNNLLTEKEVKIVNSYIETEFKKQGITITATYYCP
                     HTTEDNCFCKKPSPYFINQAVHKYNIDRKHSYIIGDHPSDSECGIRAGLTPIYVLTGH
                     GNKHKNELTCEVAICKSLSHATERILNNIYYDNKII"
     gene            2269..>3890
                     /locus_tag="PKN90_02340"
     CDS             2269..>3890
                     /locus_tag="PKN90_02340"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HNZ85150.1"
                     /translation="MTTKLFKRLQEKNVTLPDDVQILLNSCKGFTLFETVEDIADAAV
                     GGKDKLEFEVKYDVPGKGEYTEAIVHRVTNGISANYTEAYMRRRDPDTMAIGDNLPTD
                     KKRFKDKYGYEFSNLRKETFDWLKTQELAIFFYFAGNNGIGVPGIAIVPANAGFFAMG
                     LAMLQHIVPINEMEDSFAIESVLYVAPVFRHTHFEGKQVVVHNRLEQLHEIFAYNLYP
                     GPSAKKGMYAVLLDKGEKEGWVTTHCSSVQAISPYDNTTAFMHEGASGGGKSEMMQHL
                     AKELNGQVMIGKNVVTGETRAISLPIFCSFNPITDDMALCHPSFQKNPGKLSISDAEN
                     GWFIRVDSVCDYGDDPHLEKQTIKVEKPLLFLNIQSHPGGTALIWNHTEDEPGKPCPN
                     PRVILPRETVPGSIAKPVAIDIRSFGVRTPPCSSENPNYGIFGLFHILPPALAWLWRL
                     VSPRGHANPSIIGGDGMGSEGVGSYWPFATGKRVTHANLLLEQIMQTPKMRYTLVPNQ
                     YIGVWKVGSKPQLLMREYLTRRGNGNLKPEQY"
BASE COUNT         1420 a          674 c          656 g         1140 t
ORIGIN      
        1 acttaaaagc aagttgcata acactacatg taaagtgtcg cgttttaaag agcaaataaa
       61 aaaaactaaa ccacacaaac acgacaacta ttacgagcta attattttgc tcgaaggcag
      121 tggttttcat caaatagaat ctacccatta cctgattcaa gcccctgttt gttttgtttt
      181 gcaacctgga caaatgcatt attggcagtt tacttccata cccaaaggat atgtcatctt
      241 gtatagcaat gatgaatttg atgatctaca tgaaaaaaat gtatgcttat tgtataaaca
      301 attggctttt catacacaca tcaacatatc aaaagaaaca ctaccaattg ctttattaga
      361 agagctatac aaggtataca gcgaaccaaa tcatttttct caactcatca tacatgggtt
      421 attgcaatcg ttgtttgcca ctttattaat taccacacaa aaccacgacc tacaagctca
      481 atcaatgagt ttaccattcg aacagtttag caaattgatt caacaacaca tacatacaca
      541 tcacaaggta taccaatatg ctgcattatt aaacatgaca cctcaaaact tgaatgccat
      601 ctgccttaaa catggaaaaa cgaatgccgc aaaccacatt aacaatcacc tgcttttaga
      661 agccaaacga tacatcctgc atacggaaaa aaccattgct gaaatagctg acctactatc
      721 gtttagcgat tcatctcatt ttgttaagtt ctttaaaaaa tacgaaggca ttacacccgt
      781 gcaatttaga caacagtttt ttcaatagta gcacacatat ggattctttt atacatatta
      841 ctacctaaca aatactgggt gtggtacaac aatcttgcat aacctattgt tttcaatttt
      901 tgcaaacata tgcacagtac aaataaggta ggattacaaa gaagccatca tggcttgttt
      961 catattattt tacacacaat agatttattt aaagtatgtt aattttaaat taatatttta
     1021 agcgctactt aattatttaa actaaaatag tatctttgta gtcaaataat agtacacaat
     1081 gaaacgcatc ggagaaagaa taaaaaaata cagacgcatc tataatatgg cattaggaga
     1141 tttagctaca aaagttggta tcactcctag tgcattatct caaatcgaaa atgcaaaagc
     1201 atttccatct attattacac taaaatcgat tgcagaaaat ctacatacca cagttggtga
     1261 attaattgga gagaatgagg catccaaaca ttctccagta tatagagcag tagattataa
     1321 attacttgat aatcttgact acaaggctac aatctatcac attccatacg aagatgttaa
     1381 caaacaaatg gatagttttg tgataatctt aaaaccacaa gaaaaagttg aaaatatatt
     1441 tgcatcacac agcggacaac gaatgtgtta tgtgttacaa ggaaatatag aatttaccat
     1501 cgatgagaaa aaagtagaat tgaaaacagg tgatagtgca ttttttcacg cgaaaaacat
     1561 ctctatatta caaaataagc aacaagagga tacgaaactt ttattagtaa tagccccagc
     1621 aagtatgtaa aactacattt atctgatatt tttattgatg aataaaaatt tgtattaaaa
     1681 aaatttttat ctttgtgatg tgtaataggg aacagaagat aacaaaagaa aagcaaaaat
     1741 ttcctgctat atttcttgat agagatggca ctcttataga agatgtggga tacataaaag
     1801 aaccaagcgc ggtttctttt tttccaaata cattcgctga tttaatccgt ttacaagaac
     1861 actttatgct gtttattatt accaatcaat ctggtattgg aaataattta ctgacagaaa
     1921 aagaagtaaa aatagtaaac tcatatattg aaacagaatt taaaaaacaa ggaattacaa
     1981 ttacggcaac ttattattgt ccgcacacta ctgaagacaa ttgtttttgc aaaaaaccaa
     2041 gtccttattt tattaatcaa gcagtacata aatacaatat tgaccgaaag cattcttata
     2101 taattggaga ccatcctagt gatagtgaat gcggtatacg agcgggatta acacctatat
     2161 atgtattaac aggtcacgga aataaacata aaaacgaatt aacatgtgag gtagccatat
     2221 gcaaaagtct atctcatgca actgagagaa ttttaaacaa tatatattat gacaacaaaa
     2281 ttatttaaaa gattacaaga aaaaaatgta accctaccag atgacgtaca gattctgcta
     2341 aacagctgca aagggtttac cttatttgaa actgttgaag acatagcaga tgctgctgta
     2401 ggtggaaaag acaagctaga atttgaagta aaatacgacg tgccaggcaa aggtgaatac
     2461 actgaagcta ttgttcatcg tgtaacaaat ggtatttctg ccaattacac tgaagcctat
     2521 atgcgtcgtc gcgaccctga cacaatggct ataggagata atttaccaac agacaaaaaa
     2581 cgttttaaag acaaatacgg atatgaattt tctaatttaa gaaaagaaac ttttgattgg
     2641 ctaaaaacac aagaattggc tattttcttt tattttgcag gaaataatgg cattggcgta
     2701 cccggtatag ctatcgtgcc tgcaaatgct ggtttctttg ctatggggtt ggcaatgtta
     2761 caacatattg taccaattaa tgaaatggaa gactcatttg caattgagtc tgttctatat
     2821 gtagctccag tttttagaca tacccatttt gaaggaaaac aagtagttgt tcataaccgt
     2881 ttagagcaat tacatgaaat ttttgcttat aacctttatc caggtcctag tgctaaaaaa
     2941 ggaatgtatg ccgttctatt agacaaagga gaaaaagaag gctgggtaac tacacactgt
     3001 tcaagtgtac aagcaattag cccatacgat aatactactg catttatgca cgaaggggca
     3061 agtggtggtg gaaaaagtga gatgatgcag catttagcta aggaattgaa tggacaagta
     3121 atgattggaa aaaacgtcgt aacaggagaa actcgtgcta tcagtttacc aatattctgt
     3181 tcgtttaacc caattaccga tgacatggca ttgtgtcacc catctttcca aaaaaaccct
     3241 ggtaaattat caattagcga tgctgaaaat ggctggttta ttcgtgtaga tagtgtatgc
     3301 gactatggag atgatcctca cttggaaaaa caaactatta aagtagaaaa acctctatta
     3361 tttttaaata ttcaatcaca cccaggaggt accgctctta tttggaatca cacagaagat
     3421 gaacctggca aaccttgtcc aaatcctcgt gttattttac ctcgcgaaac tgtgcctggt
     3481 agcattgcca aaccagtagc tattgatatt cgtagttttg gtgtacgtac tccaccatgt
     3541 tctagcgaaa atcctaacta cggtatattt ggattattcc atatacttcc acccgcatta
     3601 gcatggttat ggagattagt ttctccacgt ggacatgcta atccaagtat tataggtgga
     3661 gatggaatgg gctctgaagg tgtaggttca tattggccct ttgccactgg taagagagta
     3721 acacatgcta atcttttgtt agagcaaata atgcagactc caaaaatgcg ttatacattg
     3781 gtaccgaatc aatacattgg tgtatggaaa gtaggatcca aacctcaatt attaatgaga
     3841 gaatacttga ccagaagagg aaacggaaac ttaaaaccag agcaatatca
//
LOCUS       DAOPUR010000092         3885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_23692_length_3885_cov_3.250653, whole genome shotgun sequence.
ACCESSION   DAOPUR010000092 DAOPUR010000000
VERSION     DAOPUR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23692_length_3885_cov_3.250653"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKN90_02345"
     CDS             <1..296
                     /locus_tag="PKN90_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNZ85151.1"
                     /translation="QHIILPISGSGIFSSYILALGRALGETMAVTMLIGNANGIPNSI
                     FDTGNTMASIIANQFGEAYGLKLSSLIAVGLLLFLITAIINFIGKLIIKRNNL"
     gene            293..1159
                     /gene="pstA"
                     /locus_tag="PKN90_02350"
     CDS             293..1159
                     /gene="pstA"
                     /locus_tag="PKN90_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNZ85152.1"
                     /translation="MNTFTYRKIKNASLYVLVCLFAGLTAVPLFAILGQVLIKGWRQL
                     IVSDFFVKSTPTTLDALIALRSGEIIPGGIANGIVGTILMVLMATFIAIPIGIFAGIY
                     LAEYKTSKYASIIQFLTDLLQGVPSIVVGVVAFIWVVIPLKSYSALAGSAALTVMMLP
                     LIIRSTEETMKMIPNTMKEAGLALGTSYPIVILRVMLPSAFGGIFTGVLLAVSRVIGE
                     TAPLMLTALGSTMIQWDVTRPNSAVSLLIWEFYNDPNLQSLVWSASLFLLIFVLTLNI
                     IAKQIAKKWRIQ"
     gene            1144..1917
                     /gene="pstB"
                     /locus_tag="PKN90_02355"
     CDS             1144..1917
                     /gene="pstB"
                     /locus_tag="PKN90_02355"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNZ85153.1"
                     /translation="MENTIKENILTLKDVCISYTPGQNAVNMVTADIQKGLITSIMGP
                     SGCGKSTLLRAINRMHELYPGITITGDIKLNNKDILKMNAIEVRRMAGMVFQRPNPFP
                     TLSIYDNVIAGYTLNGIKKTKDEKDEIVENSLKNVALWDEVKDVLQKKGTFLSGGQQQ
                     RLCIARALALKPDILLMDEPTSALDPIATNRIEELLVELKKEVTIVIVTHNMSQAARI
                     SDYSMFMYLGDLIEYDTTKKMFTNPQDKRTEEYLTGKFG"
     gene            1938..2609
                     /gene="phoU"
                     /locus_tag="PKN90_02360"
     CDS             1938..2609
                     /gene="phoU"
                     /locus_tag="PKN90_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNZ85154.1"
                     /translation="MNTIRQEYLNRLNEEFKNMSDTLIEQLELLSLQINGNNDTNLFE
                     KIKINEKSINKSEVIIREEIVNTIVLNSPKASDLRRIIAYFDMIGDMERAADLLYNTS
                     KRMTELFEKDSLYELYQSDVTFLYEKANNMIQNAIFAFLREDQDIAKNVIASDDIVDK
                     LHADLQKKMLSMTIPEKYPHMLADSLNIGRISYNIERIGDSATNIAEAVIFLTEGKDI
                     KHNHE"
     gene            2684..3373
                     /locus_tag="PKN90_02365"
     CDS             2684..3373
                     /locus_tag="PKN90_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ85155.1"
                     /translation="MKKILVVDDEIDLCEILRFNLENEGYEVDTAHSAEEALQILSDQ
                     HDLILLDVMLEGMSGYQMANKLRTELKNSIPIIFLTAKNTENDLITGFSIGGDDYITK
                     PFSLKEVLVRMKAIARRASKKTTSTHQSSVVQIGNLVIDDETKTVKIQGETIFLTKKE
                     FLILYMLVQTAGRFHTREQILNNVWNDESFVLERTVDVHIARLRKKLGTVGNQIINRA
                     GFGYSFEPDNN"
     gene            3377..>3885
                     /locus_tag="PKN90_02370"
     CDS             3377..>3885
                     /locus_tag="PKN90_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HNZ85156.1"
                     /translation="MTYKRKLIVYFLIAFCLYVIVDIGIQLNREKRFKTDSLRSNLHA
                     YTEVVENYLASTKDTASVIKLLPENLRVSLINREGVLTYDNAFDISKAENHGNRPEVL
                     AAKLRNSGSATRSSESTKIDYLYYARMLDDGSVIRTSLPYTIKLDDFLHRDSIILYII
                     ILMFVTTLF"
BASE COUNT         1357 a          690 c          677 g         1161 t
ORIGIN      
        1 tccaacatat tattttgcca atttctggtt caggtatctt ctcttcctat attttggcac
       61 ttggaagagc cttgggagaa accatggctg ttacaatgct tattggcaat gccaacggta
      121 tacctaactc tatttttgac actggaaata ccatggcaag tattattgct aatcaatttg
      181 gagaagctta cggactaaaa ttatcttcac taattgcagt tggtttgtta ctttttctta
      241 ttacagccat aatcaatttt attggtaaac tgattattaa acgcaacaac ttatgaacac
      301 gtttacgtat agaaaaatca aaaatgcatc tttatatgtg ctcgtttgct tgtttgcagg
      361 acttacggct gtgcccttat ttgccattct tggtcaagta ctgataaaag gctggcgaca
      421 actaatagta agtgattttt ttgtaaaatc aactcctacc acattagatg cattaatagc
      481 attgcgttcg ggcgaaataa tacctggagg gattgcaaat ggtattgtgg gaaccatttt
      541 gatggtacta atggctactt ttattgctat tccaattggt atatttgctg gcatttatct
      601 tgcagaatac aaaacaagca aatatgcctc tattattcaa ttcttaacag acctattaca
      661 aggcgtaccc tctattgttg tgggcgtagt agcgtttatt tgggtagtaa ttccactcaa
      721 aagttattct gctcttgcag gcagtgccgc tcttacggtt atgatgctac cgttaattat
      781 acgttctacc gaagaaacta tgaaaatgat acctaacaca atgaaagaag ctggattagc
      841 actagggaca tcctacccaa ttgtcatttt gcgagtgatg ttaccttctg catttggggg
      901 tatatttact ggcgttttgc ttgctgtatc acgtgttatt ggcgaaacag cccctttaat
      961 gcttactgca cttggaagca cgatgataca atgggacgta acacgaccaa attcagcggt
     1021 atctttacta atatgggaat tttataacga tcctaactta caaagcttag tatggagtgc
     1081 ttcgctattt ttattaattt ttgtattaac actcaatatt atcgccaaac aaatagctaa
     1141 aaaatggaga atacaataaa agaaaatata ttaacactca aagacgtttg tatatcctat
     1201 actcctggac aaaatgcagt gaatatggtt acagctgata tacaaaaagg gcttatcacg
     1261 tccattatgg gaccttcggg atgtggtaaa agtacgcttc tgagggcaat caatcgcatg
     1321 cacgaacttt atccaggcat taccattact ggagatatca aactaaacaa caaagatatc
     1381 ttaaaaatga acgccataga agtgcgacgt atggcaggta tggtgtttca acgtcctaat
     1441 ccttttccta cattaagcat ctatgacaat gttatagcag gttataccct caatggtatt
     1501 aaaaaaacaa aagacgaaaa agatgaaatt gttgaaaata gcctaaagaa tgttgcatta
     1561 tgggatgaag taaaagacgt attacaaaaa aaaggaacct tcctttctgg aggacaacaa
     1621 caacgattat gtattgctcg tgctttggct ctaaaaccag atatattatt gatggatgaa
     1681 ccaacctcag cactagaccc catagctacc aatagaattg aagagttact ggtagaacta
     1741 aaaaaagagg ttaccatcgt cattgttaca cacaatatgt cgcaagcagc acgtatttct
     1801 gattattcta tgttcatgta tttaggagat ttaatcgaat acgacacaac aaaaaaaatg
     1861 tttacaaacc cacaagataa aagaactgaa gaatacttaa caggtaaatt tggttgataa
     1921 attaaatcca tactactatg aatacaatac gacaagaata cttaaaccgc ttgaacgaag
     1981 agttcaaaaa catgtctgat acgttaattg agcaactgga attgctatcc ctacagataa
     2041 atggaaacaa tgatacaaat ctgtttgaga aaatcaaaat caacgaaaaa tcaatcaata
     2101 aaagtgaagt aatcatccgc gaagaaattg taaatacaat cgttcttaat tcgcctaaag
     2161 cgagcgattt acgccgtatt attgcctatt ttgatatgat tggcgacatg gaacgtgctg
     2221 ccgacttgct gtataatacc agcaaacgaa tgactgaatt gtttgaaaaa gattctctct
     2281 acgaactcta tcaatcagat gttacatttt tgtacgaaaa agccaataac atgattcaaa
     2341 atgctatctt tgctttttta agagaagatc aagatatcgc caaaaatgta attgcatcag
     2401 atgatatcgt agataaacta catgctgact tgcagaaaaa aatgttgtcg atgactattc
     2461 cagaaaaata cccacatatg ttggcagatt cgctaaatat aggacgaata tcatataata
     2521 tcgaacgaat aggtgatagt gccacaaata tagcagaagc agttattttc ttaactgaag
     2581 gaaaagacat caaacataat cacgaataac actaacgaat gtctgcacat gaaaaaacag
     2641 ctaattctat acaaaacaat gtataaccta aaaaaataat cgcatgaaaa agattcttgt
     2701 agtagacgat gaaattgatt tatgtgaaat ccttcgtttc aacctcgaaa atgaaggata
     2761 tgaagtagat acagctcatt ctgcagaaga ggctttgcaa atactttccg accaacacga
     2821 tttgattttg cttgatgtaa tgctcgaagg aatgtcgggc taccaaatgg caaacaaatt
     2881 acgtacggaa cttaaaaatt ctattccaat catcttttta acagcaaaaa ataccgaaaa
     2941 tgatttaatt acgggcttta gcattggtgg agatgattat attaccaaac cattttctct
     3001 taaagaggta ttggtaagga tgaaagccat agctcgaaga gcttccaaaa aaacaacaag
     3061 cacccaccaa tcttccgtgg ttcaaatagg aaatttggta atagatgacg aaacaaaaac
     3121 agttaaaatt caaggagaaa caatttttct aacgaaaaaa gaatttttaa ttttgtatat
     3181 gcttgtacaa acagctggtc gttttcatac ccgcgaacaa atactcaata atgtgtggaa
     3241 tgatgaatca tttgtattag aaagaacggt cgatgtgcat attgcccgtt tgagaaaaaa
     3301 acttggaact gttggtaatc aaattatcaa tcgtgccgga tttggatata gttttgaacc
     3361 cgataataac taaactatga cgtataaaag aaaactcatt gtgtattttt taattgcatt
     3421 ctgtttgtat gtaattgtag atattggcat tcaacttaac cgtgaaaaac gttttaaaac
     3481 agatagcctt cgttctaatc ttcatgcata tacagaggtt gtagaaaact acctagcatc
     3541 aaccaaagat acagcttctg ttataaaatt attgcccgaa aatttacgtg tatcgctaat
     3601 taatcgcgaa ggcgtactaa cgtatgacaa cgcatttgat atatccaagg cagaaaacca
     3661 cggtaatcga cctgaggtat tagcagccaa acttcgtaac tcaggctcag ccacacgatc
     3721 atctgaatct accaaaatag actatctcta ttatgctcgt atgctcgacg atggatctgt
     3781 catacgaaca tctctaccct atacaatcaa attggatgat tttttacatc gtgatagtat
     3841 catcctatac atcatcattt taatgtttgt aactacactc tttat
//
LOCUS       DAOPUR010000093         3841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24052_length_3841_cov_2.862388, whole genome shotgun sequence.
ACCESSION   DAOPUR010000093 DAOPUR010000000
VERSION     DAOPUR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24052_length_3841_cov_2.862388"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..589)
                     /locus_tag="PKN90_02375"
     CDS             complement(14..589)
                     /locus_tag="PKN90_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNZ85157.1"
                     /translation="MKKFIQLIRLQNIISLLIFQALMYYGVVVAILTKYGLSFSYISL
                     EIILLFIASAFIAAAGYVVNDYFDTKIDTINKPDKVIIGKTLSRQQAAWIFQSLFGIG
                     ILAGIVLAFLLKDVSVGFIFIVIPGLLWFYSASYKRQFLLGNLIIGLCAAVSLFLVAY
                     IQSLLLVKQYGELIHYTQIIPEILQLLNAVI"
     gene            complement(613..1140)
                     /locus_tag="PKN90_02380"
     CDS             complement(613..1140)
                     /locus_tag="PKN90_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HNZ85158.1"
                     /translation="MNKNFKEYLPHIKAFAFDVDGVLSANCIPLYPNGEPMRMANIKD
                     GYVLQLAIKLGYPIAIITGGKTAAVRTRFEGLGIQDIYLGSSIKTIDFEHFIAKHGLH
                     PTDVLYMGDDIPDLPVMKLVGVPTCPSDAAAEIKAISCYVSNYRGGEGCVRDVIEQVL
                     KAQNKWLTDEHAFGW"
     gene            complement(1137..1922)
                     /locus_tag="PKN90_02385"
     CDS             complement(1137..1922)
                     /locus_tag="PKN90_02385"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNZ85159.1"
                     /translation="MIDVVLIGAGNVATHLAIGLHKRGIHIRQIFSRTTTSAQLLAEQ
                     VGATFTSSKSAIYTDASLYICSVKDSEISTVLTNIDFHNKLLIHTAGSVALDILAPFS
                     ANYGVLYPLQTFSKSKPINWEQCSLFIEANNEKNAEFILQIASLLSTKVQIIDSEKRK
                     NLHIAAVFACNFTNHLYTLASELMLGSGANFNDLLPLIEETTNKLCTLSPYEAQTGPA
                     VRQDTNIVAEHIAQLAKTDINKATIYALLSRNIMQTHKMKNKE"
     gene            complement(1926..2462)
                     /locus_tag="PKN90_02390"
     CDS             complement(1926..2462)
                     /locus_tag="PKN90_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ85160.1"
                     /translation="MESMYQLLQHRRSCRKYQPTSVSAQDIDTLLHAALMSPSSKRIN
                     PWEFIVVTDKELLEKLSHSKEHSSSFIAEAPLAIVIMANIEKSDVWVEDCSIAAILIQ
                     LVAEDLGLGSCWVQIRNRFTKNHKISSNDYVKEVLHIPDTYAVEAIISIGYKVDERKP
                     FDEEKLQTEKIHYQHFTH"
     gene            complement(2476..3129)
                     /locus_tag="PKN90_02395"
     CDS             complement(2476..3129)
                     /locus_tag="PKN90_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNZ85161.1"
                     /translation="MNTFFKSILIATVVFFNTNKIYSQFQKEISVGFIQGTVYPTVNF
                     DPIVTQEPILRYHGGLAIRYIAEPNVGIQAELTYSQRGWNEQSSSTGSFLKEVDYIEL
                     PLLTHIYVDKKSFRFFMQLGPKIRYAIQEKDKNTYLNLSSYQHINPIDNYFDYSLLGG
                     LGIEIRTHKLGFVQIETRYDFGIGDMYSNRKSDYFARSSNQAILASINYFFPSFYVK"
     gene            complement(3135..>3841)
                     /locus_tag="PKN90_02400"
     CDS             complement(3135..>3841)
                     /locus_tag="PKN90_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85162.1"
                     /translation="QVLTPQSDLHTSIAKEFSKVFSNKLVYFLQPRKDNLQDKKEFVN
                     QLKVAFNQNKVSYRTGFLGDFSASSLDSIANSTNKELVLIVPSTNLVVVTQLSNMIQS
                     VQSSSIRIFSFSEWHQLQIQDLYQLPHYTYTNYYIDFKDSNIHAFFTKLRNTYGIAGT
                     QSVPNYALLGYDLANYFIGLLSNYGSNFESKLQEKNNGLLQMNLQFEKVSVNGGYQNK
                     GIFLQSYDQQGITTLK"
BASE COUNT         1226 a          641 c          639 g         1335 t
ORIGIN      
        1 gcagagtcag ttcttaaata acagcattga gtaattgcaa tatttcagga ataatctgtg
       61 tgtaatgaat cagttcacca tattgtttta ctaataacaa agattgtatg taagctacta
      121 aaaaaagtga tacggctgca cacaatccaa tgataaggtt acctagtaaa aattgtcttt
      181 tgtacgaagc tgaatagaac caaagaagtc ctggtatgac aataaaaata aaacctacgc
      241 ttacatcttt caacagaaat gccaatacga tacctgccaa aattccaata ccaaaaaggg
      301 attgaaaaat ccatgctgct tgctgacgcg atagtgtttt gccaatgatt actttatctg
      361 gtttattaat cgtgtcaatt tttgtgtcaa aatagtcatt gacaacatat cccgcagctg
      421 caataaaagc tgacgcaata aacagtaaaa ttatttcaag gcttatatac gaaaaggata
      481 gaccatattt agttaaaatg gcaacaacaa cgccataata cataagagcc tgaaatataa
      541 gtaaactaat aatgttttgt aagcgaatta attgaataaa ttttttcata gtgtgttatt
      601 attatttgtt ttttaccaac cgaatgcatg ttcatcggtc aaccatttgt tttgtgcttt
      661 taatacttgt tctatcacat cgcgtacaca accttcgcct ccacggtagt ttgatacgta
      721 gcaggatatt gctttaattt ctgcagccgc gtcgctaggg caggtgggca cacctacgag
      781 tttcattaca ggcaaatcgg gtatatcatc tcccatgtaa agcacgtctg taggatgtaa
      841 tccgtgtttg gcaataaaat gttcaaaatc tatcgtcttt atggatgacc ctaaatatat
      901 atcttgtatg cctaatccct cgaatcgagt acgtacagca gcagtttttc ctccagtaat
      961 gatggcaata ggatatccta attttatagc caattgtaat acatagccat cttttatatt
     1021 tgccatacgc ataggttccc cattgggata cagggggatg caattggctg acaaaacacc
     1081 atctacatca aaagcaaatg ctttaatatg gggtaagtat tccttaaaat ttttattcat
     1141 tctttgtttt tcattttatg ggtttgcatt atatttctac ttaataaggc gtaaatcgtt
     1201 gctttattga tgtcggtttt tgctagttgt gcaatatgtt ctgctacgat atttgtgtct
     1261 tgtcgtactg ctggacccgt ttgtgcctcg taaggagata gtgtacataa tttgtttgtt
     1321 gtttcctcaa ttaaaggtag taaatcgtta aagtttgctc cacttcccaa cattaattca
     1381 ctagctagcg tatataaatg atttgtaaaa ttacaagcga atacagccgc aatatgtaag
     1441 tttttgcgtt tttctgaatc aataatttgt acttttgtgc ttaatagtga agcaatttgt
     1501 aaaatgaatt ctgcattttt ttcgttatta gcttcaatga ataggctaca ttgttcccaa
     1561 ttaattggct ttgattttga aaacgtttgt aacggataca gtacaccata attagcagaa
     1621 aaaggggcta atatatctaa tgctacacta ccagcagtat gaataagtaa tttgttatga
     1681 aaatcgatat ttgttaagac cgtactaatt tcgctgtctt ttacactgca aatatacaac
     1741 gaagcatctg tataaatagc tgatttagaa gaagtaaatg tagctccaac ctgttctgct
     1801 agtaactgag cagaggtcgt agtgcgacta aaaatctggc ggatatgtat acctctttta
     1861 tgcaatccta ttgccaaatg agttgctaca ttgcctgcac ctattagtac aacatctatc
     1921 atatattagt gagtaaaatg ttgataatga attttttctg tttgcaattt ttcttcatca
     1981 aatggtttac gttcgtctac tttgtaacca atactaataa tagcttctac tgcatacgta
     2041 tctggaatgt gtaatacttc ttttacatag tcattagagc ttattttatg gttttttgta
     2101 aaacgattac gtatttgtac ccagcaagag cccaatccta aatcttctgc aaccaattgt
     2161 attaagatag cagcaatcga acagtcttcc acccatacat cgcttttttc tatatttgcc
     2221 attataacta tagctaacgg agcctcggca ataaaagaac ttgaatgttc tttgctatga
     2281 cttaattttt ctaataattc tttgtctgtt acaacaataa attcccatgg attaatacgt
     2341 ttggatgatg gagacatcaa agcagcgtgc aataaggtgt caatatcttg tgccgaaacg
     2401 gatgtaggtt ggtatttcct gcagctgcgt ctgtgttgaa gtaattggta cattgattcc
     2461 atgattgttg ttgttttatt ttacgtaaaa tgagggaaaa aaataattaa tacttgcgag
     2521 tattgcttgg ttgctagagc gagcaaagta atctgattta cgattactat acatatctcc
     2581 aataccaaaa tcgtatcgtg tttctatttg gacgaatcct aatttatgag tacgtatttc
     2641 tattcctaaa ccacctaata aactgtaatc aaaataatta tcaataggat ttatgtgttg
     2701 ataagaagat aaattgagat acgtattttt atctttttct tgtatagcat agcgaatttt
     2761 tggtcctaat tgcataaaaa aacgaaaaga ctttttatct acataaatat gtgttaacaa
     2821 aggtaattcg atgtaatcta cttcttttag gaaacttcca gtagacgagg attgctcatt
     2881 ccatcctcgt tggctatagg taagttccgc ttgtatccca acattaggtt cagcaatata
     2941 ccgaatagct agcccaccat gataccttag aataggttct tgtgtaacaa tagggtcaaa
     3001 attaacagta ggatatacag ttccttgaat aaaaccaacg gatatctctt tttgaaattg
     3061 cgaatagatt ttatttgtat taaaaaaaac aacagtcgca atcagtattg atttaaaaaa
     3121 agtattcatt tttgctattt taaagtggta atgccttgtt ggtcataact ttgcaaaaat
     3181 atacctttat tttggtatcc accattcaca gatacttttt caaattgcag attcatttgc
     3241 aataacccat tgtttttttc ttgtaatttt gattcaaagt tactaccata attagataaa
     3301 agtcctataa aataatttgc caaatcatat cctaacagtg cgtagttagg gacgctttgt
     3361 gttccagcaa ttccgtatgt atttcgcagt ttagtgaaaa aagcatggat attactatct
     3421 ttaaaatcaa tataataatt cgtatacgta tagtgtggta attgatataa atcttgaatt
     3481 tgtagttgat gccactcaga aaaactaaat atacgaatag aggaggattg tacagattgt
     3541 atcatattac tcaactgtgt tactacgact aaattggtac tgggcacaat aagtacaagt
     3601 tctttatttg tactattagc tattgagtct aaactgctag ctgagaaatc tcccaaaaaa
     3661 ccagtacgat agcttacttt attttgatta aaagcaactt tcagttgatt aacaaactct
     3721 tttttatcct gcaaattatc tttacgaggc tgcaaaaaat aaactagctt attgctaaat
     3781 actttgctaa attcttttgc aatgcttgtg tgtaagtcac tttgcggagt taatacttga
     3841 t
//
LOCUS       DAOPUR010000094         3825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24170_length_3825_cov_3.244032, whole genome shotgun sequence.
ACCESSION   DAOPUR010000094 DAOPUR010000000
VERSION     DAOPUR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24170_length_3825_cov_3.244032"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKN90_02405"
     CDS             <1..469
                     /locus_tag="PKN90_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ85163.1"
                     /translation="EEAFPPEEKVDFSSLLSHKFADDCAFKLYAILDKNETCGMLSIV
                     EYTSFVFIYYLAITPAKQGLGIGNKVLELIKKESTTPLILEVEHATQDISKRRIEFYV
                     RNGFHVNQYPYFQPALHQHTDAVPMHIMSYPHALSEEDFLTVKTALYEHVYCV"
     gene            complement(472..1239)
                     /locus_tag="PKN90_02410"
     CDS             complement(472..1239)
                     /locus_tag="PKN90_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ85164.1"
                     /translation="MNRKYRLGVVLSGGGAKGFAHLGVLKAIEEKGLKPDIIAGVSSG
                     ALMGLLYADGKSLDEIMKFFEKASLFDSISPTLPKLGLMNPSKFKRQLRSFLTAKSFE
                     ELAIPFVVNATNLTDGKMTVFRTGESLVEAVIGSSSVPLLFTPQIIEGKQYVDGGVFC
                     NMPVSPIRDECDYIIGVHVNPICTETKAESIIEIAERVLHYAIQSSTINEKKLCDVVI
                     DVKEVKKYGMFNLDKKQEIFDYGYTKAKEVLENFSIE"
     gene            1510..2208
                     /locus_tag="PKN90_02415"
     CDS             1510..2208
                     /locus_tag="PKN90_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ85165.1"
                     /translation="MSTFRILVVDDEEDLCDILQYNLEKDGYIVDIAYSAEDALKKDL
                     TQYNLFLLDVMMGAMSGFKLGSMLKKDETTSHIPIIFITAKDTETDTLTGFTIGADDY
                     ITKPYSVKEVVARVRVVLKRTTEQKDGTPMSKDKILRFEQLFVNLTTKTTLIDKQEIN
                     LTKKEFEILSLLMSQPNNVFSREDILQQVWNNADVTDRTIDVNITRLRKKIEPYDKNV
                     VTRLGYGYCFETSN"
     gene            2224..>3825
                     /locus_tag="PKN90_02420"
     CDS             2224..>3825
                     /locus_tag="PKN90_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ85166.1"
                     /translation="MILFKKLLLSFVGIFVFFAAIIFIINVQYEKHMVKARYTETLQF
                     YTEIIDLNTKTARKTEIPNALVDELQKKDVYISIYNTSGECIYSTPMNDSVIYNVSER
                     AFGMITEKNTPEHTLVVRYDQTKSSYFFYGKKYDTHTILASIPYEYIISDVWKIRNFT
                     ASFFMFFVLSLSSLAFYLVSNKLLHTVKNLKKVILKMENKEDFDDDLLFLNKEFKEIS
                     SFIVNTYKNLTATKDALSLEKEKLFKHLQISQEGLAIYTFDRKEILANENFIQYINLI
                     SNKKISTSDSIFSLAEFSPITSFLNENQYNQNNPNEVTDKIVEIDKNDYTLLVRCIIF
                     QDKTFEISINDISKQVQNDLIKKQLTQNISHELKTPVSSIQGFMETLIENPDMEQEKK
                     DFFIQRCHAQAVRLSYLLRDISLLNKLDEGSYTFEKEKIVLNEIIEGVLKDVSLELEQ
                     KNIRVEMFLKHNMTIMGNYSLLYSIFRNFIDNSLHYAGNDISIIINCYRTDKDFYYFS
                     YADTGVGVSENHLTRIFDRFYRIDKG"
BASE COUNT         1394 a          684 c          570 g         1177 t
ORIGIN      
        1 cgaagaagcc tttccgccag aagaaaaagt agatttttct tctttattgt cgcataaatt
       61 tgctgatgac tgtgccttta agctatatgc aatcctagat aaaaatgaaa cttgtggcat
      121 gctgagtata gtagaatata cttcttttgt atttatttat tatttagcaa ttacgcctgc
      181 aaaacaagga ttaggtatag ggaataaagt acttgagtta ataaaaaaag agagcacaac
      241 accccttatt ctcgaagtag aacatgctac acaagacatt tcaaaacgaa gaattgagtt
      301 ttatgtacgt aatgggtttc acgtcaacca atacccctat tttcaaccag cactgcatca
      361 acatacagat gctgtcccca tgcatatcat gagctatccc catgcacttt cggaagaaga
      421 ttttttgact gtcaaaacag cattatatga acatgtatat tgtgtatagt cttactctat
      481 agagaaattt tctaatactt ccttagcttt cgtatagcca taatcaaaaa tttcttgttt
      541 tttatctaaa ttaaacatac catacttttt tacttccttt acatcaatta caacatcgca
      601 taattttttt tcgttaatcg tgcttgattg tatggcataa tgaagtactc gctctgctat
      661 ttcgataata ctttcggctt tggtttcggt acaaattgga ttaacatgaa ctccaataat
      721 atagtcacac tcatcgcgaa ttggggaaac aggcatatta caaaaaacac ctccgtccac
      781 atattgtttc ccttcgataa tctggggagt aaataataac ggaacactcg aagagccaat
      841 aactgcttct actaaagatt cacctgtacg aaacaccgtc attttgccgt cagtaagatt
      901 cgttgcattc actacaaaag gaatagcaag ctcttcaaat gactttgccg ttaaaaaact
      961 gcgtaactga cgtttaaatt ttgaaggatt cattaaaccc aatttaggca gggttggcga
     1021 tatggagtca aacaaggagg ctttctcgaa aaatttcata atttcatcta agcttttacc
     1081 atcagcatac aatagcccca ttaaagcacc tgaacttaca ccagcaataa tatcgggctt
     1141 cagtcctttt tcttcgatgg cttttaacac gcccaaatga gcaaaaccct ttgctccacc
     1201 accactcaat acaactccca atcggtattt tctattcatc gtacaatata tttaatggta
     1261 caaaaataca ctatatttgc gtacattata ctataaaaaa agcaaattac tatgccatat
     1321 tacaattata taacattcta catgttgcca aaatgaatac taacaccaca aaatcatctg
     1381 attatatgta tcctacgact catatacctc tatgaaatct atactaaaac atattcagca
     1441 agaaaatatt atcgcttaac gtgcaaaata gcaataaaat aaatatcttt gtaaaaaaac
     1501 ctatgcacta tgagtacatt tagaattttg gtagtagacg acgaagaaga tttgtgcgac
     1561 atactacaat acaatttaga gaaagacgga tatatcgtag acattgctta ttctgccgaa
     1621 gacgcattaa aaaaagacct aacacaatac aatctatttt tactggatgt aatgatggga
     1681 gctatgtcgg gctttaaact cggttctatg ctcaaaaagg acgaaacaac ctcgcatatt
     1741 ccaatcattt tcattacagc taaagataca gaaacagaca ccttaactgg ttttacaata
     1801 ggggctgatg attatattac aaaaccttac tcagtgaaag aagtagttgc tagagtgcga
     1861 gttgtattaa aacgaactac ggagcaaaaa gacggaaccc ccatgtcgaa agataaaatt
     1921 ttgcgttttg agcaattgtt tgtgaactta actactaaaa caacactcat tgataaacaa
     1981 gaaataaatt taacaaaaaa agaatttgaa attttaagct tattaatgag ccaacccaac
     2041 aatgtttttt ctcgagagga cattttgcaa caagtatgga ataacgctga tgttaccgac
     2101 cgaactattg atgtaaacat tactcgactg cgaaaaaaaa tagaacctta tgataaaaat
     2161 gttgttactc ggctaggata tggatattgt tttgaaacat caaactaaca agcctccaca
     2221 accatgattt tgtttaaaaa actattatta tcattcgttg gtatttttgt gttttttgcc
     2281 gctattattt ttattatcaa tgtgcaatac gaaaaacaca tggtaaaggc acgttataca
     2341 gaaacattac aattttatac cgagataatt gatttaaata caaagactgc tagaaaaact
     2401 gaaattccca acgcactggt agatgaattg caaaaaaaag atgtatacat ctctatttat
     2461 aataccagtg gcgaatgtat ttattccaca ccaatgaatg attcggttat ttacaatgta
     2521 tctgaacgtg cattcggaat gattacagaa aaaaacacac cagaacatac attagttgta
     2581 cgttacgacc aaacaaaatc ctcctacttc ttttatggaa aaaaatatga tacacacacg
     2641 attttagcct caattccata cgaatatatt ataagcgacg tttggaagat tcgtaatttt
     2701 acggcttcat tctttatgtt ttttgtacta agtttatcaa gcttagcgtt ttatcttgta
     2761 agcaataagc tattgcatac cgtaaaaaac ctcaaaaagg taattttaaa aatggaaaac
     2821 aaagaagatt ttgatgacga tttacttttt ttaaacaaag aatttaaaga gatatctagt
     2881 tttatcgtaa acacatataa aaaccttaca gccaccaaag atgccttatc gttagaaaaa
     2941 gaaaaacttt ttaaacatct tcaaatatcg caagaaggct tagcgattta cacttttgat
     3001 agaaaagaaa ttttggcaaa cgaaaatttt atacaataca ttaatttgat ttcaaacaaa
     3061 aaaatcagca catcagatag tattttttca ttagccgaat tttcccctat cactagtttc
     3121 ttaaatgaaa accaatacaa tcaaaataat cccaatgagg taacagacaa aattgtagaa
     3181 atagacaaaa acgattatac cttgctcgtt cgatgtatta ttttccaaga taaaaccttt
     3241 gagatttcaa ttaatgacat ctccaaacaa gttcaaaacg acttaataaa aaagcaactc
     3301 acacaaaata tttctcacga actgaaaacg cctgttagta gtattcaggg ttttatggaa
     3361 acattgatag aaaatcccga catggaacaa gaaaaaaaag atttttttat tcaacgttgc
     3421 catgctcaag ctgttcgttt aagctatttg ttacgagaca tttcgttact caacaaactc
     3481 gacgaaggtt cttacacatt tgaaaaagag aagattgtat taaatgagat tattgaagga
     3541 gtactaaaag atgtttcgct cgagttagaa caaaaaaaca ttcgagtaga aatgtttcta
     3601 aagcacaata tgacaataat gggcaactat tcgttactat attccatctt tagaaatttt
     3661 attgacaata gcctacatta tgctggcaac gacattagta ttatcattaa ttgttaccga
     3721 acagacaagg atttctacta tttttcgtat gctgacacag gagtgggggt atctgaaaac
     3781 catttaaccc gtatttttga ccgtttttat cgtattgaca aagga
//
LOCUS       DAOPUR010000095         3819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24221_length_3819_cov_4.505313, whole genome shotgun sequence.
ACCESSION   DAOPUR010000095 DAOPUR010000000
VERSION     DAOPUR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24221_length_3819_cov_4.505313"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKN90_02425"
     CDS             <1..651
                     /locus_tag="PKN90_02425"
                     /inference="COORDINATES: protein motif:HMM:NF039230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNZ85167.1"
                     /translation="GGDENDLDPSDNTDDATTSPLHPLPVANDDVAYTNENTAITIDV
                     TKNDSFGAPNAPKTGTVVITTHPDASKGTATVNNNGTPTNPTDDKIVFTPKPGYFGDV
                     TMEYEITNYNGVKERATVTITVSGMRIPDGFSPNGDAINDTFVIQRSSNLTINLIVFN
                     RWGNKVFEQANYANDWEGTGINNQPLPEGTYYRIVEAMNNTTGQTQKLSGVITLKR"
     gene            675..1598
                     /locus_tag="PKN90_02430"
     CDS             675..1598
                     /locus_tag="PKN90_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNZ85168.1"
                     /translation="MKKILFIFFACIASYVVAFAQQAPMYSQYMFNMLNVNPAYAGNR
                     AVQNVTLLHRHQWVGFEGNPRTSSLSWDMRAEDSNNGFGAQIYNDQLGVENTTGLQGF
                     YSYKIPLKNASFALGLSAGLLNFSAAYTEVTTIDPNDPAFAENANTFLPTVGVGLLYA
                     SEKWYLGLSVPALLRTKDFLAEAEALVNKFGATNHYFITGGYVVEVNPNLALKPSLLF
                     KAVSGAPLQTDININAWWNQKWGAGASFRTGDAVVAMLEYQITNTIRLGYAFDYSISQ
                     LNAFNQGTHEIMLRYEFDTSKAIRVLSPRYY"
     gene            1646..>3819
                     /locus_tag="PKN90_02435"
     CDS             1646..>3819
                     /locus_tag="PKN90_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ85169.1"
                     /translation="MMKNISYKIRLTLACVLLSATSVQLIAQTNNDILKQADRHYQYA
                     NYARAVQLYEQVVAQTKLLVTQEATPEQTEKLQQEKIDLFTKIADSYWQMRQYNKAKV
                     ALDEIKNISLVKLSNKEKNRLAQLYALTGDYKKAADIYDQIPGAKNKAELYRQDDFFG
                     KLKADSADWTIQLLNINSAYNDFSPVIVHQNILFSSNKPQGKEKRAFGWDGNDYTSLW
                     LFPIKDLKNIQEKPTQKSDKKTTTLQTKKIAGIYTPGDVRPMEQRSSLKNEMPLLPTT
                     QSGKGVRVSGTEKFQFNIATASVDSLGNIFLSVNNQKVKNTQTSSISIMQGSWANQAI
                     SQVKALPLDGFFATMHPTVNKQSTLLIFTAKIAESDSYDLYMSKRNDANQAWSVPVKL
                     AENINSTGNEVFPYIAPDGYLYFSSDGHPGLGGLDLFKIDLQQAINKTGTIESLGYPV
                     NSAADDFGWAQDTTKRAGFFSSNRYANADNIFSYAYLPIPKSMFVSGLVKEKKSLDLL
                     PGATVFLVDKEKQIVYVDKADKDGRFMFEIKNHGEFTLKGLEENYFEDCIDINVPAPA
                     YKQNQYAAKDILLELQVPKKWVLENLLYDLDKWNIRKDAQAPLDSVVAIMKKYPIFVE
                     LGSHTDSRGSDTYNERLSQRRAQSAVDYIVSKGISRSRITAKGYGETQPTNECKNGVN
                     CSEEQHQANRRTEIKVTHNPEPAHTLDPNKFKKGDILKTSDFP"
BASE COUNT         1251 a          797 c          759 g         1012 t
ORIGIN      
        1 ggtggagatg aaaatgatct ggacccatca gacaatacag atgatgccac aactagccca
       61 ttgcatccac taccagtggc aaatgatgat gtagcatata ctaacgaaaa tacggctatt
      121 accattgatg ttactaaaaa tgatagtttt ggagctccta atgctccaaa aacaggaact
      181 gttgttatta cgactcaccc tgatgcaagc aaaggaactg caacagtaaa caataacgga
      241 acacctacga accctactga tgataaaatt gtatttaccc caaaaccagg ttattttggc
      301 gacgtaacaa tggagtacga aattacgaat tacaatggag ttaaggaaag ggcgactgta
      361 acgattactg tctctggcat gcgtatcccc gatggttttt cacccaatgg cgatgctatc
      421 aacgatacgt ttgtgataca acgtagctcc aacttaacca tcaaccttat cgtatttaac
      481 cgatggggta ataaagtgtt tgagcaagct aactatgcca acgattggga gggtactggt
      541 atcaacaacc agcctctacc cgaagggact tactaccgta ttgtagaagc tatgaacaac
      601 actactggac aaacacaaaa actatctggt gtgattactc ttaaaagata agaatcgact
      661 gaaatttact aattatgaaa aaaatactat tcatcttctt tgcttgtatc gctagttatg
      721 ttgtggcttt tgctcagcaa gctcccatgt actcgcagta tatgtttaat atgctcaacg
      781 tgaaccctgc ctatgccggc aacagggcgg tgcaaaacgt aacgctttta cacaggcacc
      841 aatgggtggg ctttgaagga aatcctcgca catcatccct ttcgtgggac atgagggctg
      901 aagattctaa taatgggttc ggagctcaaa tttataacga ccaactaggt gtggaaaaca
      961 ccaccgggtt gcagggattt tattcctaca aaataccttt aaaaaacgct tcttttgccc
     1021 ttgggttaag tgctggattg cttaatttct ctgccgctta cacagaggta actaccatag
     1081 acccgaatga ccctgctttc gctgaaaatg caaatacatt ccttcctacc gtaggtgtgg
     1141 ggctattgta tgcttccgaa aaatggtatc taggactctc tgttcctgcc ctgctcagaa
     1201 caaaagattt cttggctgaa gcagaagctc tggtaaataa gtttggggct actaatcact
     1261 attttattac gggggggtat gttgttgagg taaatccaaa tttagctctc aaaccatcgc
     1321 tattattcaa agctgtttcg ggggctcctt tgcaaacaga cattaacatc aatgcttggt
     1381 ggaatcaaaa atggggggct ggggctagtt tccgtactgg cgatgccgtt gtagcaatgc
     1441 tcgaatacca aataacaaac accattcgtt tggggtatgc tttcgattac tctatttccc
     1501 aactaaatgc ttttaaccaa ggtacgcacg agattatgct ccgttacgaa ttcgatacga
     1561 gcaaagccat aagggtatta tcgcctcggt attactaaca gggtgcttac ttacattgta
     1621 taacgtcttt aattacttac taatcatgat gaaaaatata tcctacaaaa taagactaac
     1681 gctagcctgt gtattgcttt ctgctactag tgtacagctt atagctcaaa caaacaacga
     1741 tatcctcaaa caagctgaca ggcattacca atatgctaat tatgctagag cggtgcaact
     1801 atacgaacag gtggtggcac aaacaaagct gctggtaacg caagaggcta cgcccgaaca
     1861 aacagaaaaa ttgcaacaag aaaaaattga cctgtttacc aaaattgctg atagctactg
     1921 gcaaatgaga caatacaaca aggctaaagt tgctctcgat gaaataaaaa atataagcct
     1981 tgtgaaatta tcgaacaaag aaaaaaacag gctcgctcaa ctctatgctc ttacaggtga
     2041 ttacaaaaaa gctgccgata tttacgacca aattccagga gcgaaaaaca aagctgaact
     2101 ctatagacaa gatgatttct ttggcaaact aaaagccgac tctgccgatt ggactatcca
     2161 gctccttaat attaacagtg catacaacga tttctcccct gtcattgtac atcaaaacat
     2221 ccttttctct tctaacaaac cccaaggtaa agaaaaaagg gcattcggat gggatggtaa
     2281 tgattacacc tctttatggc tattccctat aaaagaccta aaaaatatac aagaaaaacc
     2341 cactcaaaaa agtgataaaa agacaacaac gctacaaaca aaaaaaatag ctggcatata
     2401 tacccctggt gatgtgcgtc ctatggagca acgcagttcc ctcaaaaatg aaatgccttt
     2461 gcttcctact actcaatcgg ggaaaggtgt gcgtgtgagt ggtaccgaaa aattccaatt
     2521 caatatagcg actgcgagtg tagattcttt aggaaatatt ttcttatctg ttaacaacca
     2581 aaaagtaaaa aatacacaaa ccagttctat ttccattatg caaggttcgt gggctaacca
     2641 agctatatcg caggtaaaag ctctgccttt ggatgggttt tttgctacca tgcaccctac
     2701 cgttaacaaa caaagtaccc tccttatttt tactgctaaa atagcagaat cagactcata
     2761 cgacttgtat atgagcaagc gtaatgacgc taatcaagca tggagtgtgc ctgtaaaatt
     2821 ggctgaaaac atcaactcta cgggaaatga ggtgtttcct tatatagctc ctgacggata
     2881 cttgtatttt agctcagacg ggcatcctgg attaggggga ttggatttat ttaaaataga
     2941 cttacaacaa gctatcaata aaaccggtac tattgaatcc ctcggatatc ctgttaactc
     3001 tgctgctgat gattttgggt gggctcaaga taccactaaa agggctggct tcttctcctc
     3061 taataggtat gccaatgcag ataatatttt ttcgtacgcc tacttaccta ttcctaaaag
     3121 tatgtttgtg tcaggattag taaaagagaa aaaatcactc gatttattgc ctggggctac
     3181 tgtgtttttg gtggacaaag aaaaacagat tgtgtacgtg gacaaagcgg acaaggatgg
     3241 tcgtttcatg tttgagataa aaaatcatgg cgagtttacg ctaaagggat tagaagaaaa
     3301 ctattttgaa gattgcatcg acattaacgt gccagctcct gcttacaaac aaaatcaata
     3361 tgcggcaaaa gatattttgc tcgagttgca agtacctaaa aaatgggtgt tagaaaatct
     3421 attgtacgat ctcgataaat ggaacatacg caaagatgca caagctcctt tggacagtgt
     3481 ggtggctatt atgaaaaaat accccatttt tgtagaactg ggttcgcata ctgacagtcg
     3541 tgggtccgat acgtacaacg aacgtttatc ccaacgcagg gctcaatcgg cggtcgatta
     3601 tatcgtgagc aaaggaattt ctcgctcacg tattacagcc aaagggtatg gcgaaacaca
     3661 accaaccaac gaatgtaaaa acggcgtaaa ctgttccgaa gagcaacacc aagcaaacag
     3721 acgtacagaa ataaaggtta cacataaccc agagcctgct catacgcttg acccgaacaa
     3781 atttaaaaaa ggagacatac tcaaaacatc cgacttccc
//
LOCUS       DAOPUR010000096         3819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24226_length_3819_cov_3.518597, whole genome shotgun sequence.
ACCESSION   DAOPUR010000096 DAOPUR010000000
VERSION     DAOPUR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24226_length_3819_cov_3.518597"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PKN90_02440"
     CDS             complement(<1..760)
                     /locus_tag="PKN90_02440"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ85170.1"
                     /translation="MGSMLDMSQPVQIDIASILKGKTGGKKPSSMLVYLLSKLLHVKE
                     INHFLTLAKDKKNLDFFQALIEYLNVRVLVSGVDNLPLSGTPCVFVSNHPLGGIDGVA
                     VAYEVGKHYPEGIIIPANDLLMYLKNVQDLFIPVNKVGGQAKNLAEALHVAYMSSKQM
                     MMFPSGMVSRKSKGKIEDPEWKKNFIVKAVEYKRNIVPIYIGARNSTLFYWVARVRKK
                     LKITLNIEMLLLPHEMFRQRNKELKIVIGKPIQWN"
     gene            937..1395
                     /locus_tag="PKN90_02445"
     CDS             937..1395
                     /locus_tag="PKN90_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNZ85171.1"
                     /translation="MTSLIGNIEAKVDAKGRAFVPAQFRRQLQDETKFILRKGLFHDC
                     LVLYTEQAWNDIITVLRNNLSRWNRREEQIFRQFVAEADRIELEENGRMLIPKRYIDA
                     LQIDKDIRFVGADTVIEIWPINKVENTFIDADNFALEIENLMNIGKSTKD"
     gene            1488..2390
                     /gene="rsmH"
                     /locus_tag="PKN90_02450"
     CDS             1488..2390
                     /gene="rsmH"
                     /locus_tag="PKN90_02450"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNZ85172.1"
                     /translation="MTTYHTPVLLKESIEGLLTNKSGVYVDLTFGGGGHSRFLLSQLH
                     SDAKVFGFDQDKDAEMNAVNDSRFTFVQSNFCYLENFMQYYGINEVDGILADLGVSSH
                     HFDDAERGFSFRFDGILDMRMNQAAKLTAKEILNTYTEQQLADIFFYYGELPLARKIA
                     RKIVEQRKNEQFETIFGFVGIIQPLIGKEKEKKQLAQVFQALRIAVNNEMDALKKMLL
                     SSAKVLKKEGRFVCITYHSLEDRLVKNYLKTGNMEGNINKDFFGNIQTPFSLINNKVI
                     IPTTEEIASNSRARSAKLRIASKK"
     gene            2395..2769
                     /locus_tag="PKN90_02455"
     CDS             2395..2769
                     /locus_tag="PKN90_02455"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNZ85173.1"
                     /translation="MAKIKTLKKKIKESEEFNDLTKTSVKDIFDGNVFSKSYFLKQIK
                     LIVLVSFFIFFYMDNRMECEKQIATINDLNQQLTDVKYTSMVVEADLLSISRRTQIEA
                     LLHQHAIELKELSIPPYKLTTK"
     gene            2774..3544
                     /locus_tag="PKN90_02460"
     CDS             2774..3544
                     /locus_tag="PKN90_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85174.1"
                     /translation="MKAVLFILLAIAVVAIISLLVLLFIRKNTVKKEIMLRFAIIYIF
                     ILIAFGGVIFRIVHLQYVEGEKLRALTASQQSALRPIKPNRGNILASDGRLLASSIPT
                     YYLYMDLMADALVQKSTKNEAEFKQDVRRLSVALAKKFKDKTAWEYEKQFLNTFYDQT
                     NKRKREYLLYPKPISYIDLAEVKTFPILQKGRYGGGFYTKKRVTRIKPFGELASRTIG
                     DIYGEEEKGGSSGLEKQYNKLLQGERILLSQKNGWKIC"
     gene            3525..>3819
                     /locus_tag="PKN90_02465"
     CDS             3525..>3819
                     /locus_tag="PKN90_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ85175.1"
                     /translation="MAGKFVDIVVKDAVNGYDVVSTIDLDIQDIVDSELRKKLIEIDA
                     QSATAIVMEVQTGQIKAISNLTRREEGVYLETTNIALADEVEPGSTFKTLALMI"
BASE COUNT         1418 a          599 c          666 g         1136 t
ORIGIN      
        1 cattccattg aataggttta cctataacaa ttttcaactc tttatttctc tggcgaaaca
       61 tttcatgtgg caacaatagc atttctatat taagggttat tttcaatttt ttacgcacac
      121 gtgctaccca ataaaacaaa gttgaattac gtgctcctat atatataggt actatgttac
      181 gtttatactc tactgctttt acaataaaat tttttttcca ctcagggtct tctatttttc
      241 ctttcgattt acgcgaaacc attcctgacg gaaacatcat catttgctta gatgacatat
      301 atgccacatg taaggcttct gccaaatttt ttgcctgccc tcccacctta ttaaccggga
      361 taaacaaatc ttgtacattt tttaaataca tcaacaaatc attggctggg ataattatcc
      421 cctcaggata atgcttacct acctcatacg caactgctac gccatctata ccacctaatg
      481 gatgattgga cacaaacaca caaggcgtac ccgatagagg taaattatcg actcctgaaa
      541 ctaaaacacg cacattgaga tactctatta atgcctgaaa aaagtccaaa ttttttttat
      601 ccttagctaa agttaaaaaa tgatttattt ccttcacatg caataactta gacaacaaat
      661 aaaccaacat tgaagaaggt ttcttccccc cagtctttcc ttttaatatt gatgcaatat
      721 ctatttgcac tggctgactc atgtccaaca tactacccat tctcattata atgccgacaa
      781 atatacattt ttttttccat gcaaagattg aatacacaat ttttttataa aaatctataa
      841 aataaagcct gttaataact ttgtggggaa ttgtgtcgta aaaagttgca aaatgtcgta
      901 aaaagtatta attttacact tcaagaagag caagatatga catcattaat tggaaatata
      961 gaagcaaaag ttgacgcaaa aggcagagcc tttgttccag cccaattcag aagacaattg
     1021 caagatgaaa ccaaattcat tttacgcaaa ggattgtttc atgattgctt ggtattgtat
     1081 acagaacaag cttggaatga tattattact gtgttgcgaa acaacttaag cagatggaac
     1141 agacgtgaag agcaaatatt tcgacagttt gtagccgaag ccgatcgcat tgaattagaa
     1201 gaaaacgggc gaatgcttat tccaaaacgg tatattgatg ccttacaaat tgacaaagat
     1261 attcgttttg ttggagcaga taccgtcatt gaaatttggc ctattaataa agttgaaaat
     1321 acctttattg atgcggataa ttttgcactt gaaatagaaa atttgatgaa tattggcaaa
     1381 tctacgaaag attaagaaat tgcgaagaca caatatttcg cacatatata tacaataatc
     1441 gcaaatatat aataatggtt aacaaataaa aaaaacgaaa atatagaatg acaacgtatc
     1501 acactcccgt tcttttaaaa gaaagtattg aaggtctact cactaacaaa tcgggggtat
     1561 atgttgattt aacgtttggt ggaggtggtc attctcgttt tttactcagt caactacata
     1621 gcgatgcaaa agtatttgga tttgaccaag ataaggatgc agaaatgaat gcagtaaatg
     1681 atagccgatt tacgtttgta caaagcaact tttgctactt agaaaatttt atgcaatact
     1741 atgggattaa cgaagtggac ggaatattag ctgatttagg ggtatcttct catcattttg
     1801 atgatgcaga acgcggtttt tcattccgtt ttgatggaat attagatatg cgcatgaatc
     1861 aagctgcaaa acttacagca aaagaaattt taaacacata cacagaacaa caacttgcgg
     1921 atatattctt ttattatgga gaattacctc tggcaagaaa aatagcgaga aaaatagtag
     1981 agcaacgaaa aaacgaacaa tttgaaacca tttttggatt tgtcggcata atacaacctt
     2041 tgataggaaa agaaaaggaa aaaaaacaat tggcacaagt atttcaggca ttacgaatag
     2101 ccgttaacaa cgaaatggat gcattaaaaa aaatgttact gagtagtgca aaagttttaa
     2161 agaaagaagg tcgtttcgtt tgcattacct atcattcatt agaagatcga ttggtgaaaa
     2221 attatttaaa aacaggcaat atggaaggga atataaacaa agactttttt gggaatatcc
     2281 aaacgccatt tagcctgata aacaacaaag taattattcc aacgaccgaa gaaatagcaa
     2341 gcaattcacg agcaagaagt gcaaaactac gcatagcaag caaaaaatag cagtatggca
     2401 aaaataaaaa cattaaaaaa gaaaataaag gaaagcgaag aatttaacga tttaacaaaa
     2461 acttcggtta aagatatttt tgatggaaat gtattttcca aaagctattt tttaaaacaa
     2521 attaaattaa ttgtattggt tagttttttt atattttttt atatggataa ccgtatggaa
     2581 tgtgaaaaac aaattgcaac tatcaatgat ttaaatcaac aattaactga tgtaaaatat
     2641 acatcaatgg tagtagaagc tgatttgctt agtataagta gaagaacaca aatagaagca
     2701 ttattacacc aacatgctat tgagttaaaa gaactaagta ttccaccgta taaattaaca
     2761 acaaaataac acaatgaaag cggttttgtt tatattactt gcaattgcag tagttgctat
     2821 cattagcttg ctggtgttat tgtttatacg taaaaatacg gtaaaaaaag agattatgct
     2881 tcgttttgca attatctaca tttttattct aattgcattt ggaggagtta tatttagaat
     2941 tgttcatcta caatatgtgg aaggagaaaa attacgggca ctaacagcta gccaacaatc
     3001 tgcattgcga ccaattaagc caaacagagg taatatttta gcgagtgatg gacgcctttt
     3061 agcgagctca attcctacgt attatttgta tatggattta atggctgatg ctttggtgca
     3121 aaaaagcaca aagaatgaag cagaatttaa acaagacgtt agacgtttat cagtagcatt
     3181 agcaaaaaaa tttaaagata aaacagcatg ggaatatgag aaacaatttt taaacacttt
     3241 ttacgaccaa acaaacaaac gtaaaagaga atatctgttg tatcctaaac ccatatcata
     3301 tattgattta gctgaagtaa aaacgttccc aattttgcaa aaaggaagat atggtggagg
     3361 tttttataca aaaaaacgag taacccgcat caaacctttt ggagaactag cctcacgtac
     3421 tattggagac atctatgggg aagaagaaaa aggaggtagt agcggcttag agaaacaata
     3481 caacaaactt ttgcaagggg aaaggattct actatcgcag aaaaatggct ggaaaatttg
     3541 ttgatattgt ggtgaaagat gctgtgaatg ggtatgacgt tgtaagcact attgacttag
     3601 acattcaaga cattgttgat agcgaactaa gaaagaaatt gattgaaatt gatgcacaat
     3661 cggctactgc tattgtaatg gaagtgcaaa caggtcaaat aaaagcaatt agcaatttaa
     3721 ctcggagaga agaaggtgtt tatcttgaaa ccaccaatat tgccttggct gacgaagtag
     3781 agccaggttc tacttttaaa acattggctt tgatgatag
//
LOCUS       DAOPUR010000097         3804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24360_length_3804_cov_2.902641, whole genome shotgun sequence.
ACCESSION   DAOPUR010000097 DAOPUR010000000
VERSION     DAOPUR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24360_length_3804_cov_2.902641"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..844
                     /locus_tag="PKN90_02470"
     CDS             359..844
                     /locus_tag="PKN90_02470"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNZ85176.1"
                     /translation="MKKISLHTAIEVYTYEELTDQQKTLIQKAKETSLSAYSPYSKFS
                     VGAAILLENGEIVCGSNQENAAYPSGICAERTAMFYANSTYPTVAPICIAIVAFTDGK
                     FKQTAISPCGACRQVLIESEIRFNKPITMLLYGEPEIFFINSITDLLPLNFAPSSLLS
                     S"
     gene            857..1243
                     /locus_tag="PKN90_02475"
     CDS             857..1243
                     /locus_tag="PKN90_02475"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNZ85177.1"
                     /translation="MTKEKISHEYNLRTTISLTHMWQYFHTEDGLGTWFADKVISKNG
                     IYEFFWGNTSQKAKILHKTQQFSVRFHWIDDEEETTYFQFKLERDDITNVVTLTITDF
                     SYPDEREDTIQLWNTQLEKLRRTIGL"
     gene            1369..2847
                     /locus_tag="PKN90_02480"
                     /pseudo
     CDS             1369..2847
                     /locus_tag="PKN90_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            2858..>3804
                     /gene="ribB"
                     /locus_tag="PKN90_02485"
     CDS             2858..>3804
                     /gene="ribB"
                     /locus_tag="PKN90_02485"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNZ85178.1"
                     /translation="MTTFKLNSIEEAIEDFKEGKFLIVVDDEDRENEGDFIIAAEKIT
                     PEAVNFMLINGRGVLCAPITEERCAELELEKQVSNNTSILGTPFTVTIDKIEGCSTGV
                     SAADRAATIKALADPTSTPNTFGKPGHISPLYAKTKGVLRRAGHTEASIDLARLAGLY
                     PAAALIEIMNEDGTMARMPDLQKVAEKFSIKIITIKDLIAYKIKQESIVEKGVTVHMP
                     TQYGMFKLTPFRQKSNGLEHIALTKGEWKDDEPILVRVHSSCATGDIFGSLRCECGEQ
                     LHHSMKMIEKEGKGAIVYLYQEGRGIGLMNKIKAYALQD"
BASE COUNT         1372 a          607 c          644 g         1181 t
ORIGIN      
        1 agcaataggg ctaaattttt cgatataagt ttggtatttg ggatttctat attgctgaga
       61 aaaaagagct atcgttggaa cgatacatac aaaaaaaaga aaggtaaatc gtattaattt
      121 catgcttagg aactctcact atttaaaatt ataaacccat taataatcgt cgataaaata
      181 atagatacaa aaatagtatt ttagttacga aaaatgctaa aaaaattgtt tttttagtga
      241 attttagtta tgtttgtaaa atatggatag tatgatataa aagtttctaa ttgatagaaa
      301 atttatctaa cgaagcaaat actaaataaa tctgtactaa aacattaatt gtaacataat
      361 gaaaaaaata agtttacata cggcaattga ggtatataca tacgaagagc ttaccgacca
      421 acaaaaaaca ctcatacaaa aagccaaaga aacctcttta tctgcctata gcccatattc
      481 aaaatttagt gttggtgctg ctattttact agaaaatggg gaaatagttt gtggctctaa
      541 tcaagaaaat gcagcgtatc cctcaggcat atgtgctgaa cgaacagcta tgttttatgc
      601 aaattctact tatccaacgg ttgctcccat ttgtattgct attgtagctt ttactgacgg
      661 aaaatttaaa caaacagcta tatctccatg tggagcatgc agacaagtac taatagaatc
      721 tgaaattcgt tttaataaac ctataactat gctgctatat ggagaacctg aaattttttt
      781 cattaacagc ataacagact tattgcctct caattttgct ccttctagtt tgttatcttc
      841 ataataccaa aagcttatga caaaagaaaa aatctctcac gaatataacc ttcgtacaac
      901 catttcgcta acacatatgt ggcaatattt tcatactgaa gatggtttgg gcacttggtt
      961 tgcagacaaa gtaattagta aaaatgggat atacgaattt ttttggggaa acacttccca
     1021 aaaagcaaaa atactacata aaacacaaca attttctgtt cgattccatt ggatagatga
     1081 tgaagaggaa actacttatt ttcaatttaa attagaacgt gatgatataa caaatgttgt
     1141 aacacttact atcactgatt tttcatatcc agatgaacga gaagatacaa tacaactatg
     1201 gaatacccaa ctggaaaaac ttcgtagaac gattggttta taaaaacatt tgattcaaaa
     1261 ataacaacag aatacgtttt gttaacactt taaatactaa aattgcatat ttttctgtaa
     1321 ttttgtgctc tatttcgtga aatgggtaaa aacatacagc aattaagaat gccaattgta
     1381 aaaaaaataa attattatat tataaagaat tttctaggag tatttattat tactttttta
     1441 gggagtttat ttatccttgt actccagttc tttttacaac acattaacag tttggttggg
     1501 aaaggcattg gactaggagt gtttattgaa ttattttctt acgcagggct aacactcatc
     1561 cctcttgctt tacgttaagt aattttaatt ggctcattga tgacctttgg gaatttagga
     1621 gaacgattag agctactggc aatgaaagct gcaggaattt ctctttggaa aattatgcaa
     1681 cctcctatta tactggttgg ttgcatatca atctctggat tttatttttc taataatatt
     1741 ctacccaaga cccaagtaaa aatgtggacc ttagtattct ccatacgaga gaaatctcct
     1801 gaattagata tacctgaggg agttttttat acagagatac ctggtagaaa tattttcata
     1861 ggtgaaaaga gccataaaaa caatacactt aaagacatca ttatatacga tttctctaga
     1921 ggatttacca atacaagtat tgtggttgct gactctggaa aaatgagttt tagtgttgac
     1981 aaaacaaatc ttcttatttc tttgtacaaa ggggaagcct ttgaaacatt accaaataac
     2041 gcaaaaacta gaaatgctgt tgtcccatat cgcagagaat tatttgaaca aaaagaaatt
     2101 atcattgatt ttgatgcaaa cttcaatttg cttggaacag aattacttga aggtcaatat
     2161 gtaagcaaaa atgcatcgca actacaacac tctatcgact ccatttccac aaaaatagac
     2221 gaagaaaaaa acaaatatcg ggaagatttt ttgcgaaata agtactataa ctcatacaca
     2281 ggatcttcct cggcttttgc aattttagac tcaaccatag atgctaaagg atataatttt
     2341 caagacattc tccttaatac agaaaatggt ttgcaagaac aagttatgct accttctatt
     2401 aatcgagcat acactataaa aaatgattta atatacaatc aatccattat aaatgctctt
     2461 ctatttgacg ctcgtaaaca ctccattgaa tggtataaaa agtttacact ttcttttgca
     2521 tgtatcatat tcttttttat aggagcacca ttaggagcca tcatccgcaa aggaggaatg
     2581 ggtttgccta tcgttatttc aattgtgcta ttcattgtat attacataat tgataatact
     2641 ggatacaaaa tggctcgtga aggtatatgg gaagtaggta tgggaatgtg gtttagtgca
     2701 ttggtattgc tccccttagg tatgttctta acctataaag ctgttactga cgctgttatt
     2761 cttaccaccg aaggcaaact atttaatcaa aaaaaatgga taaacgctct aattgcatta
     2821 gtcaagaaaa aaaagaaaaa tatttaacta aaaaaatatg actacgttta aattaaattc
     2881 tattgaagaa gcaatagaag atttcaaaga aggtaaattc ctaattgtag tagacgatga
     2941 agacagagaa aatgaaggag acttcattat agctgccgaa aaaattacac cagaagctgt
     3001 aaattttatg ttaattaatg gtcgtggtgt gttgtgtgct cctatcacag aagaacgttg
     3061 tgccgagctt gaattagaaa agcaagtttc aaacaatacc tcaatacttg gaactccttt
     3121 taccgttacc atagacaaaa ttgaaggttg ttcgacggga gtgtctgctg ctgacagagc
     3181 cgctacaatc aaggctcttg ccgatcctac ttctactcca aatacatttg gcaaacctgg
     3241 acatattagt ccgctatacg caaaaaccaa aggcgtactc agacgtgctg gacacaccga
     3301 agcatctatt gatttagctc gtttggcagg cttgtatcct gctgcagctc taatcgaaat
     3361 aatgaacgaa gatggcacaa tggcacgtat gccagatttg caaaaagtag cagagaaatt
     3421 tagtataaaa ataattacaa ttaaggattt aatcgcctat aaaattaaac aagaatcaat
     3481 tgttgaaaag ggtgtaactg tacatatgcc tactcaatat ggaatgttta aactaactcc
     3541 gtttcgccaa aaatcaaatg gattagagca cattgcatta accaaaggag aatggaaaga
     3601 cgatgagcct attttagtac gtgttcactc atcgtgtgct acgggagata tttttggctc
     3661 attgcgttgc gaatgtggtg aacagctaca tcatagtatg aaaatgattg aaaaagaagg
     3721 aaaaggtgca atcgtatatc tttatcaaga aggaagaggt attggtttaa tgaacaagat
     3781 taaagcgtat gccctacaag acaa
//
LOCUS       DAOPUR010000098         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24487_length_3789_cov_3.071505, whole genome shotgun sequence.
ACCESSION   DAOPUR010000098 DAOPUR010000000
VERSION     DAOPUR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24487_length_3789_cov_3.071505"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..1118
                     /locus_tag="PKN90_02490"
     CDS             357..1118
                     /locus_tag="PKN90_02490"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNZ85179.1"
                     /translation="MKIKKILVSQPAPENDKSPYYDIAEKYNVKIEFRPFIKVEPLPL
                     KDFRQQRISILEHTAVIFTARTAIDHFFSICEELRITVPETMKYFCVTETIANYLQKF
                     IDYRKRKVFFPKTGKQEDIIIPVTKHKTEKFLLVVSDVHTDKFVSLLEKNTIQYTKGV
                     FYRTVSNYFSPEEAFDYDMLLFFSPAGVDSLFKNFPNYQQGEVYIGCFGTTTALAVKN
                     AGLRLDVEAPTKESPSMTSALDAFLKEKAKMNGKK"
     gene            1195..1602
                     /gene="rnpA"
                     /locus_tag="PKN90_02495"
     CDS             1195..1602
                     /gene="rnpA"
                     /locus_tag="PKN90_02495"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNZ85180.1"
                     /translation="MKTLRFTFPKKEHLCGKTTIDKLFEKNNSFLQYPLRVLYKIEKN
                     PDNKIPAKILLSVSKKYFKRAIHRNTLKRKMREAYRKQKSPLILLLSEKNLSITMAIL
                     YISKEPISYEQIDAGMKECITKLMHKIENTTRK"
     gene            1602..1823
                     /gene="yidD"
                     /locus_tag="PKN90_02500"
     CDS             1602..1823
                     /gene="yidD"
                     /locus_tag="PKN90_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNZ85181.1"
                     /translation="MKKLLQTILLIPIYIYRGIISPIKPPSCRYTPTCSEYAIQAIKK
                     HGPLKGMHLAIRRLLSCHPWGGSGYDPVP"
     gene            1840..2484
                     /locus_tag="PKN90_02505"
     CDS             1840..2484
                     /locus_tag="PKN90_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNZ85182.1"
                     /translation="MYYNIHSHKVAKYDNTITIYNVSLHNDITISALSSIGIAPWESD
                     LYTKTNVALLTKYGINENVVAIGEVGLDKRIDTPLTQQLAIFTHQILIAEQCNKPMII
                     HCVGYYNELIQLFRKRRPTVKYILHGFRGKSPLAQELVKHGFYISLGEKSVLHQESIQ
                     QIPLNKILIETDDSEIHVEEVYKSISSIKNISVEELKKSIEENITQCFGWNLRN"
     gene            complement(2489..>3789)
                     /locus_tag="PKN90_02510"
     CDS             complement(2489..>3789)
                     /locus_tag="PKN90_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNZ85183.1"
                     /translation="YTNFGFGSVLIFLLIGTILTIVVQSSSATMAITLIMCSKGWISF
                     ELGAAMVLGENIGTTITANIAAIPANVSSKRAALAHSLFNIFGVIWMLFVFYAFIDMV
                     ATLVQNYGPGDPTQLQAMTKTIDSETLNLISTADSSSLSVDQLALKEKFEGSQLATSY
                     ALSVFHTAFNIINTFIMIFFVGLIAKTVSWIIPKKKSDEEFHLTHISTGLLSTSELSI
                     IQATKEVNVYAERTLRMFYMVKELYVEKDVQAFQKTYDRIAKYENISDRMEVEIAQYL
                     TKVAEGRLSDESKNRLQSILRMVSEIESIGDSCYNMARIIKRRNEDKSEYTEQMNANV
                     EKMFDSIIKAYDEMMHVLADFDTKTSVDLGYCKEIESEINELRNALKMQNVVDVKGKK
                     YSYQASVTYMDLIIECEKLGDYIINVEEAVKDSTTLTKKI"
BASE COUNT         1355 a          688 c          542 g         1204 t
ORIGIN      
        1 aaatatatct tgttgaaact ctatgtgtat acattttttt caacaacaaa cagtctcgct
       61 ataaggcaat atttttcaat cattttctat agtggacaac ttacctttct gtttcttttg
      121 ggaattttat ttagtaatga atacgtacac atatacctat attacggggt agccatacta
      181 gccttattaa cgttattgtc aatgttttat atcatataca gaactttttt cagtaaaata
      241 catttagttt tatattttat tttgtacctt tgcaccctgg aaattttacc cttatgttta
      301 cttataaagt tttttgtagg ggacatatat tttatcaacc attaaattgt aaagcattga
      361 aaatcaagaa aatactcgta tctcagccag ctcctgaaaa cgataaatct ccgtattatg
      421 atatagcaga aaaatacaat gtaaagatag aatttcgtcc attcattaaa gtggaacctc
      481 tgcctttaaa agatttccga caacagcgca tatctatttt agaacatact gctgttattt
      541 ttactgctcg caccgctatc gaccattttt tttcgatatg tgaagaactg cgtattaccg
      601 tgcccgaaac aatgaaatat ttttgcgtaa ccgaaacaat agcaaattac ctacaaaaat
      661 ttattgatta ccgtaaacgt aaagtttttt ttccaaaaac aggaaaacaa gaagacatta
      721 taatacccgt tacaaaacat aaaacggaaa agtttttgtt agttgtatct gatgtacata
      781 ctgataaatt tgtgtctcta ttagaaaaaa atacaattca gtacacaaaa ggagtattct
      841 atcgtacggt gagcaattat ttttcgcccg aagaggcttt tgactatgat atgttgctat
      901 tttttagtcc tgcaggagtt gactctttgt tcaaaaattt ccctaattat caacaaggag
      961 aagtgtatat tggatgtttt ggcacaacca cagccctagc tgtaaaaaat gcaggattac
     1021 gattagatgt agaagctcct acaaaagaat ctccctcaat gacaagtgct ttggacgctt
     1081 tcttaaaaga aaaagcaaaa atgaatggaa aaaaataata ccattttgaa gtaatagtat
     1141 ctctcattga ttagcttgaa aaaaaaagct atctttgaga gatatttttt tagtatgaaa
     1201 acacttcgtt ttacctttcc taaaaaagaa cacttgtgtg gaaaaacgac cattgataaa
     1261 ttgttcgaaa agaacaattc atttttgcag tatcctttac gtgtattata caaaatagaa
     1321 aagaatcccg acaataagat tcctgcaaaa atcttactat cagtatccaa aaagtatttt
     1381 aaaagagcaa tacatagaaa tacactaaaa agaaaaatgc gagaagctta tcgcaaacaa
     1441 aaaagccctt taatactgct tttatctgaa aaaaatttat ctattacaat ggctattcta
     1501 tacatttcta aagaacctat aagctatgag caaatagatg cagggatgaa agagtgtatc
     1561 acaaaactca tgcacaaaat tgagaataca acacgaaaat gataaaaaaa ctattacaaa
     1621 ccatactgct aataccgata tacatctaca gaggcatcat ttctcctatc aaacctccgt
     1681 catgtaggta tacccctacc tgctctgaat atgcgataca agcaataaaa aaacatggac
     1741 ctttgaaagg aatgcattta gcaattagac gtttattaag ctgtcacccg tggggaggta
     1801 gcggatacga tcctgttcca taattctaat acgaagaaaa tgtattacaa tatccactcc
     1861 cataaggtag caaaatacga taataccatt accatatata atgtttctct acataatgat
     1921 attactatct ccgcattgtc atctattggt attgctcctt gggaaagcga tttatacact
     1981 aagacaaatg tagcattgtt aactaaatat ggtataaacg aaaacgtggt tgccattggg
     2041 gaagtgggtt tagataaacg aatagacaca cctttaacac aacaattagc tatcttcaca
     2101 caccaaattt taatagcaga gcaatgcaat aaaccaatga taatacactg tgtaggttac
     2161 tataacgaat taatacaatt atttcggaaa cgccgaccta cagttaagta tatcctacat
     2221 ggatttagag gaaaatctcc attagcacaa gaacttgtta aacatggatt ttacatttca
     2281 ttgggagaaa aaagcgtact tcaccaagag tctattcaac aaatacctct gaacaaaata
     2341 ttgatagaaa cagatgattc tgagatacat gtagaagagg tttacaaatc tatttctagt
     2401 ataaaaaaca tttcagtaga agagctaaaa aaaagcattg aggaaaatat cactcaatgc
     2461 tttggatgga acttgcgaaa ttaagacatt atattttctt tgttaatgtt gtggagtctt
     2521 ttaccgcttc ttcaacattg ataatataat cacccaattt ttcacattct ataattaaat
     2581 ccatatacgt tacactcgct tgatatgaat attttttccc ttttacatcc acaacatttt
     2641 gcattttcaa ggcatttctt aattcattta tttctgattc aatctcttta caatagccca
     2701 aatcaacaga tgtttttgta tcaaaatcag caagcacgtg catcatctca tcatacgctt
     2761 taataataga atcaaacatc ttttcaacat tcgcattcat ttgctcagta tactccgatt
     2821 tatcttcatt tctgcgtttt ataatacgtg ccatattata acaactatct ccaatgcttt
     2881 ctatttcaga caccatacgt aaaatacttt gcaaacggtt tttactctca tcactcaatc
     2941 tgccttctgc caccttggtt aaatattgag caatctcaac ctccattcta tcgctaatat
     3001 tttcatactt agcaattcta tcataggttt tttgaaaagc ctgcacatct ttttctacat
     3061 acaattcttt caccatataa aacatacgca acgtccgttc ggcatacaca tttacctctt
     3121 tagttgcttg aataatagac aattcggaag tagaaagcaa acctgtagat atatgtgtaa
     3181 gatggaattc ttcgtctgat ttctttttag gaattatcca agataccgtt ttagcaataa
     3241 gacctacaaa aaatatcata ataaaagtat taataatatt aaatgcagta tgaaacacag
     3301 ataatgcata ggatgttgcc aattgacttc cttcaaattt ttcttttaat gccaattgat
     3361 ctacagaaag agaggacgaa tcggctgtac taataagatt taacgtttca gaatcaattg
     3421 ttttagtcat agcttgcaac tgtgtagggt cgccaggtcc ataattttgc accaaagttg
     3481 ctaccatatc aataaaagca taaaacacaa acaacatcca aataacacca aatatattaa
     3541 ataatgagtg agccaaagct gctcgttttg aagaaacgtt agcaggtata gcggcaatat
     3601 ttgcagtaat ggtagtcccg atattttcgc ctaataccat cgcagctccc aattcaaatg
     3661 atatccaacc tttactacac ataatcagcg taattgccat agtagcagaa gaagactgca
     3721 ctacgatagt taatattgtt cctatgagca agaatatcaa cacagaacca aacccaaagt
     3781 tggtatatt
//
LOCUS       DAOPUR010000099         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24537_length_3784_cov_2.430142, whole genome shotgun sequence.
ACCESSION   DAOPUR010000099 DAOPUR010000000
VERSION     DAOPUR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24537_length_3784_cov_2.430142"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKN90_02515"
     CDS             complement(<1..683)
                     /locus_tag="PKN90_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNZ85184.1"
                     /translation="MSQSEKQIFCTDATMCAILAKCNSQNKWELWGLSEVSETGEAKY
                     EIRLFSDYEFDEVDAINTTSGRNQIKVLFQTNWYEIVFIEHKKAINMPIEPEWYCMGN
                     FSVEPISKSFSLLKLSFTYEDENESIESLIARGAGKNQTVLIDQINITEWHTSETRFE
                     RLIDVIDYFLKNGISINAKNELGETALLAAAATDCNTAVVNLLLQHGANINDKDIFGC
                     TALVKACET"
     gene            1369..2172
                     /locus_tag="PKN90_02520"
     CDS             1369..2172
                     /locus_tag="PKN90_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNZ85185.1"
                     /translation="MKKLFAVLAVIFSLTFGLTFSAFAQDAAPAVDTAAVEQVAVEEA
                     LVEEAAAPVAEEGGIHQALKQKFIEGGAGFMASIALCLILGLAVCIERIIYLSLATSN
                     SKKLLEEVEAALAQGGVEAAKEICRNTRGPVASIFYQGLTRIDEGIDVVEKSIISYGS
                     VQTTLLEKNLSWIGLFIGLAPMLGFLGTVIGMIEAFDRIQQAGDISATIVAGGIKVAL
                     ITTVGGLIVAIILQVFYNFIVSRIESVVSDMEDSSISMLDILVEQSNKK"
     gene            2200..2652
                     /locus_tag="PKN90_02525"
     CDS             2200..2652
                     /locus_tag="PKN90_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85186.1"
                     /translation="MLNNLSKITLYVLIGITVVVSVLFFWGGAVDEYAEYIEPNYTQA
                     MMILMYVLFGIAILITLVSLLIAFLENFKVDKKTATKPLISIFALLALVGFAWLIADG
                     SPMQILGLDVQPTENEIKFVDVQIKAIYLLSGVAVLSMFLSFFTKRLK"
     gene            2690..3280
                     /locus_tag="PKN90_02530"
     CDS             2690..3280
                     /locus_tag="PKN90_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNZ85187.1"
                     /translation="MSRNKRKVPEINSSSMADISFLLLIFFLVTTTMVVNKGLARRLP
                     PPIPPDQKTEDLKVKERNVLVVLLNSDNQLMVQREYISVTELRKKAKEFIVNANNAQD
                     MPEKKAEEVQFFGEVPLTRDHVISLQNDRTTSYKAYIEVQNELIAAYNELREELAKSK
                     FGVSYAELDDDRKTAIQYIYPQKISEAEPKNYGGNK"
     gene            3280..3762
                     /locus_tag="PKN90_02535"
     CDS             3280..3762
                     /locus_tag="PKN90_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNZ85188.1"
                     /translation="MGKFNKNSSSRELPTISTASLPDIIFMLLFFFMATTQMKEVDFK
                     VSIKEPKATELEALEKKNLVRFIYVGTPTDKYRGVYGSETRVQLDDAFADVSQIEDYI
                     VAQRAAMNEYEQSLLTVALRVDEDAKMGIVTDIKQALRRAYALKINYVSRQRDNSQAF
                     "
BASE COUNT         1233 a          538 c          675 g         1338 t
ORIGIN      
        1 aatgtttcac aagcttttac cagtgctgtg cagccaaaaa tatctttatc attgatgttt
       61 gcaccatgtt gcaacaatag gtttacaact gctgtattgc aatcagtagc cgcagccgct
      121 aacaatgcgg tttcaccgag ttcatttttt gcatttatag atataccatt tttcaaaaaa
      181 taatcaataa catctattag gcgttcaaaa cgtgtttctg atgtgtgcca ttcagtaata
      241 tttatttggt caattaaaac tgtttgattc ttgccagcac ctcgtgctat taaggattca
      301 atgctttcat tttcatcttc atacgtaaag ctcaatttta acaatgaaaa agattttgat
      361 attggttcaa cagaaaaatt tcccatacaa taccattcag gctcaattgg catgttgata
      421 gcttttttat gttcaataaa tactatttcg taccaattcg tttgaaaaag aactttaatc
      481 tgatttcgtc cacttgttgt atttattgca tctacttcat caaactcgta gtcagaaaat
      541 aggcgtattt cgtattttgc ttcgcctgtt tccgatacct cactcaaacc ccacaattcc
      601 catttgtttt gagaattgca ttttgctaaa attgcacaca tagttgcgtc tgtgcaaaaa
      661 atttgttttt ctgattggct catttttttc attttataaa tatatttccc tatataatac
      721 gaaaaagtgg tgatagtaaa aaaaaagaaa gcgaatttat gtgtgtaaaa attacgtaat
      781 atactgaatg aaagttaatt aacctgttga taactttttt tcatctccta ttattttgac
      841 tcttttttga tgtggttttt aaataaaata tttgttcaag ttaatttatt ttagagttgt
      901 ttgggtgtgt ttttttaatg cttgatttct tctgtgtaaa gattaatgaa tttgtcatca
      961 aaattatacc taccatttat tatttatcaa ttctatcttt atattggatt ttttgattca
     1021 atctattagt aatatacaat tgtgtaaaac tactgaagtt tttttaattt caaattttta
     1081 aggcgttttt tctttattca aagattttaa aaacacacag ttttttctac attgcctttt
     1141 attcatattt tcgtaattaa aagtgttatc gtagttttat acaagctttc aaaaaataat
     1201 tgcataaaaa ttgttttgta ttctttcttt ttttattaac tttgagccct gaatgttttt
     1261 tagtttttct cttgtttaga aagaataagt aaaaactacg taatttaata aagggaataa
     1321 attaatcatt ttaaataaaa caagtaatta atcaaaacac aaaaaaaaat gaaaaagttg
     1381 tttgcagttt tagcagtaat ttttagtctt actttcggtt tgactttctc agcgtttgca
     1441 caagacgctg ctccagcagt tgatacagca gctgtagagc aggtagccgt agaagaagcg
     1501 ttagtagaag aagccgcagc tcctgtagct gaagaaggtg gcattcacca agcattaaaa
     1561 caaaaattta tcgaaggtgg tgctggtttt atggcatcaa ttgcattatg tcttatttta
     1621 gggctagccg tatgtattga gcgtattata tatttaagct tagctacttc aaattctaag
     1681 aagttattag aagaagttga agctgcattg gcacaaggag gtgtagaagc tgccaaagaa
     1741 atatgtcgca atacaagagg tccagttgct tctatctttt atcaaggctt aacacgtatt
     1801 gacgaaggta ttgacgtggt agaaaaatct attatttctt atggaagtgt tcaaacaact
     1861 ttactagaaa agaatttatc ttggataggt ttgtttattg gattggctcc tatgttagga
     1921 ttcttgggaa cagtaattgg tatgattgaa gcttttgacc gtattcaaca agcaggtgat
     1981 atttctgcaa ctatcgtagc aggaggtatc aaggtagctt tgattactac tgttggaggt
     2041 cttattgtag ctattatttt acaagtattt tataacttta ttgtttcgcg tattgaatca
     2101 gtagtaagcg atatggaaga ttcttcaatt tctatgttag atattttagt cgaacaaagc
     2161 aataaaaaat agattatttt acaattaaaa aataaaaaca tgttaaacaa tctttcgaaa
     2221 ataaccctct atgtcctaat aggcatcaca gtggtagttt ctgttttgtt cttttggggt
     2281 ggagcagtag atgaatatgc tgaatacata gaaccaaatt atacacaagc aatgatgatt
     2341 ttaatgtatg tgttgtttgg tatagctatt cttattaccc ttgtatcgct acttattgca
     2401 tttttggaaa attttaaagt agataaaaaa accgcaacca agccattaat tagtattttt
     2461 gctttattag cattggtagg ttttgcttgg ttaatagcag atggcagccc gatgcaaatt
     2521 ttaggtttgg atgttcagcc tactgaaaat gaaattaagt ttgtagatgt gcaaataaaa
     2581 gcaatctatc tattgtcggg tgtagcagtg ttgtcaatgt ttttaagctt ctttacaaaa
     2641 agattaaaat aagttgtaat acacgaatca tattaaatta ataaatagaa tgagtagaaa
     2701 taaaagaaaa gtacctgaaa ttaattcaag ttcgatggcg gatatttcct tcctgctctt
     2761 gattttcttt ttggtaacta ctactatggt ggtaaataaa gggcttgcta gaagattgcc
     2821 tccacccatt ccgcctgacc aaaaaacaga agatttaaag gtaaaagagc gtaatgtatt
     2881 ggtagtgttg ctgaattcgg ataatcaatt gatggtgcaa cgtgaatata tcagtgtaac
     2941 tgaattgcgt aagaaagcaa aagaatttat cgtaaatgcg aataatgctc aagatatgcc
     3001 tgaaaaaaaa gctgaagaag ttcaattttt tggagaagtt cctcttacac gggatcatgt
     3061 gatttctctt caaaatgata gaactacttc gtataaagca tacattgaag tgcaaaatga
     3121 gcttatagct gcatataatg aattgcgtga agaattagct aaatcgaagt ttggagtatc
     3181 gtatgcggag ctagatgatg atagaaaaac agccatacaa tacatttatc cacaaaaaat
     3241 ttcggaagcg gaaccaaaaa attatggagg gaataagtaa tgggaaaatt taataaaaat
     3301 agtagttcga gagaattgcc tactattagt accgcatcat tgcccgacat tatttttatg
     3361 ttgttgttct tttttatggc tactacacaa atgaaagagg ttgactttaa ggtgtctatt
     3421 aaagagccta aagctactga attagaagct ttggaaaaga agaatttggt tcgctttatt
     3481 tatgtaggta ctccgactga taaatacaga ggtgtatatg gatctgaaac gcgtgtacaa
     3541 cttgatgatg cttttgctga tgtatcgcaa atcgaagatt atattgtagc tcaacgtgcg
     3601 gctatgaatg agtatgagca atcgctacta actgtagctc ttagagttga cgaagatgct
     3661 aagatgggca ttgttacaga tattaaacaa gctttgcgta gagcatatgc cttaaagatt
     3721 aattacgttt ctagacaacg cgataatagc caagcttttt aaaatcaaca gtgatggttg
     3781 aaat
//
LOCUS       DAOPUR010000100         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24584_length_3778_cov_4.044588, whole genome shotgun sequence.
ACCESSION   DAOPUR010000100 DAOPUR010000000
VERSION     DAOPUR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24584_length_3778_cov_4.044588"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2611)
                     /locus_tag="PKN90_02540"
     CDS             complement(<1..2611)
                     /locus_tag="PKN90_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNZ85189.1"
                     /translation="MCSAKSNSYNFSLKAFSQTFLFLFLLSTLPIFSQGVGQYPVQVS
                     TVVLPNPPMYLSAYSGVETDKLSVTLLNRDTQQSSLQVKLRMRIYCSQGQEISSRAES
                     HYTPITLEGGIPLRLSMSDLAAYFRTENLRIAGSLPQGKLPVGHTRFCFEVLEYYTGR
                     LLSSQGCGSAFIVLQTPPLLQQPSHGSLLTDDPTGGILFRWSPQHSVGLERVQYEFIL
                     KEMFDTQQSAELGFAHGVEVYRTHTTATSLLYGMMQPPLIAGKRYVWQVRAFTDPISG
                     TPLAFENNGQSRVFTFDIDYPCQAPQHIIATAHTNGIHLTWDASPHPSASYHLSYRRK
                     DFSSTWHQAVTTSNSYTISNLTPGANYEYKVGISCTNKTNDIHYSTVAHIAYNPDDSE
                     TPSTSIQTKVKECGKSTPNNISNTEPKETLWAGDVIYAGDFTITLSTVQGSNGRFTGE
                     GKVHVPYLSGIGMSVYFSDISINTDNQLIEGKIHTVYDESRGTIIDLDAFDHGGTTTS
                     AQGVLVPTYKFDFALTDVTRIHYDPLTNSLSFYDATGSRVGNYELTGTAPSFPVLVSD
                     KEGNMYSINETNKDNPPANYTLVAVGKQIGELAGNAFDRNVVDSHKGTVTFFDAGVGT
                     YSFDEFLPPYDNINYIRYSSENGNERLYPQLGANYYVPWKFIPLNTHDKVQAKISLAP
                     NSELQPERVVFITESGTQVPASYNASNRTYQVSVTGTSNSGVHTLYALYPKGDGTSYF
                     HLGQLRIDSRPPQHLKLVLVYAGGSYDKIAVANELNTIYNRVGIHWQVSEVQNFTYDK
                     ETIKTLFDQKSGLLSAYNDRMTSLNQALQSHLGDSYQADACYVFMADASQAGNDRDVV
                     GFMPRGRQYGYIYT"
     gene            complement(2922..>3778)
                     /gene="fabD"
                     /locus_tag="PKN90_02545"
     CDS             complement(2922..>3778)
                     /gene="fabD"
                     /locus_tag="PKN90_02545"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNZ85190.1"
                     /translation="GAQFVGMGKDMYENSALAKEMFEKANEVLGFRITDLMFEGTDED
                     LRQTKVTQPAVFLHSVILAKTMGDKFAPDMVAGHSLGEFSALVAAGALSFEDGLVLVS
                     KRAMAMQKACEIEPSTMAAVLALDDAKVEEICASINGVVVAANYNCPGQLVISGSIAA
                     VEEACANMLEAGAKRALKLPVGGAFHSPLMEPARVELAAAIEKAQIVAPICPIYQNVT
                     ASAETDPKVIKAKLVEQLTAPVRWTQSVQNMIADGATLFVEVGPGKVLQGLVKKINKD
                     SQVQSIED"
BASE COUNT          931 a          819 c          845 g         1183 t
ORIGIN      
        1 tggtatagat atacccatac tgcctgccac ggggcataaa gcctactaca tcgcggtcgt
       61 tgcctgcttg gcttgcatct gccataaata cataacaggc atcagcttgg tagctatcgc
      121 ccaaatggct ctgcagggct tggtttaggc tggtcatacg gtcgttgtag gcacttagta
      181 agccactttt ctggtcgaac agggttttaa tagtttcttt gtcgtaagta aaattctgca
      241 cctcgcttac ctgccaatgg atacctacgc ggttgtagat ggtattcagc tcattggcta
      301 ctgctatttt gtcgtagctg cctcctgcat ataccagtac cagcttgagg tgttgtggtg
      361 ggcggctgtc gatacgtagc tgtcctaaat ggaaataact tgtgccatcg cctttcgggt
      421 acaaggcata cagcgtatgt acgccactgt tggatgtgcc tgttacgctt acttgatagg
      481 tccggttgct cgcattatag cttgctggta cttgcgtgcc cgattcggtt ataaacacaa
      541 ctcgctcggg ttgtaattcg ctattcgggg ctaagcttat cttggcttgt accttgtcgt
      601 gggtattgag tggaataaac ttccacggca cataatagtt ggctccaagt tgtgggtaca
      661 gtctttcgtt accattttcg gaagaatagc gtatgtaatt tatattgtcg tacggcggta
      721 aaaactcatc gaaactatat gttcctacac ctgcatcgaa aaatgtgacg gtgcctttgt
      781 ggctatccac tacattgcgg tcgaatgcat tgccggctaa ctcgcctatt tgtttgccta
      841 ccgccactaa ggtatagttg gctgggggat tatccttgtt tgtttcgtta atgctataca
      901 tattcccctc tttgtcgctt actagcactg ggaagcttgg ggctgtacca gttagttcgt
      961 agttgcctac ccgtgagcct gtggcatcgt agaaacttag gctattggta agggggtcgt
     1021 agtggatacg ggtaacatcg gtcagtgcaa aatcaaactt gtaggtaggc actaatacgc
     1081 cttgtgctga tgtggtagta ccgccatggt cgaaggcatc gaggtctatg attgtacctc
     1141 tggactcatc gtacacggta tgaatctttc cttctatcag ttggttatcg gtattgatgc
     1201 taatatcgct aaaatacacc gacatgccta tgcctgataa ataaggtaca tgtacctttc
     1261 cttcgcctgt aaagcgtccg ttactgcctt gtacggtgct tagggtaatg gtaaaatcgc
     1321 ctgcataaat cacatcgcct gcccataagg tttctttggg ttcggtattg ctgatgttgt
     1381 ttggcgtgct tttgccacat tcttttacct tggtctgtat gcttgttgag ggtgtttcgc
     1441 tatcatcggg gttgtaggct atatgggcta ctgtgctgta gtgtatgtcg ttggttttgt
     1501 tggtacaact tatccctact ttgtattcgt aattagctcc tggggtcagg ttgctaatgg
     1561 tatagctatt gctggtagta actgcctggt gccatgtgct ggaaaaatct ttccgtctgt
     1621 agctcaggtg gtagctcgct gatgggtgtg ggctagcatc ccatgtaagg tgaatgccat
     1681 tggtgtgggc tgtagcaatg atgtgttgtg gtgcttggca tgggtaatcg atatcgaagg
     1741 taaacacgcg actttgcccg ttgttctcaa aggctaaggg ggttccgctg attgggtcgg
     1801 taaaggctct tacctgccac acatagcgtt tgcctgctat cagtggcggt tgcatcatgc
     1861 catacagtaa gctggtagcg gtggtgtggg tacggtacac ttctacgcca tgggcaaatc
     1921 caagttcggc actctgttgg gtatcgaaca tttcttttag gataaattcg tactgtactc
     1981 gttctagtcc tacgctgtgt tgcggactcc aacgaaacaa tatgcctcct gtggggtcgt
     2041 ctgtcaatag gctgccatgg cttggttgct gcagcaatgg tggggtttgc agtacaataa
     2101 aggcactccc acagccttga ctcgacagta acctgccggt atagtattct aacacttcaa
     2161 aacaaaagcg ggtatgtcct actgggagtt tgccttgtgg taagctacct gctatacgca
     2221 ggttttctgt tctgaaatag gctgccaaat cggacatgct caaacgcaac gggatgcctc
     2281 cctctaaggt gataggggta tagtggctct cggctcggct actgatttct tgtccctgag
     2341 aacagtaaat acgcatacgc aacttgactt gcaaactgct ctgttgcgta tctctattga
     2401 gcaaggtaac ggatagttta tcggtctcta ctccactgta tgcggataaa tacataggtg
     2461 gattgggcaa tactactgta ctgacctgaa caggatattg tcctactccc tgcgaaaata
     2521 tggggagcgt ggataataaa aataggaata agaatgtttg agaaaatgct tttaacgaga
     2581 aattatatga gtttgatttt gcagaacaca ttacttcgta tattcaatta attatctact
     2641 tttttttatt gtctatgttc acattatgtt tttatacgta aaaacgatat tttacatata
     2701 attaatcatt caaaccatat tatgagaata cgatgtttct tgaaaaatag attgcctcct
     2761 tttaaaaaga taaaaaccac gcttacacaa tggtatcatc gtgtaagcga aaaatcaata
     2821 actattcaat aataaaattt gagaataagc taactatagg taaataattc aagcaaaaga
     2881 aactctattc tttacacaag ctatagctac aacaagccct cttaatcttc tatcgattgt
     2941 acttggctat ctttgtttat ttttttcacc aaaccttgca atacttttcc tggtcctact
     3001 tctacaaaga gggttgctcc atcggcaatc atattttgta cagattgtgt ccagcgaaca
     3061 ggtgctgtaa gttgctctac taatttggct ttaattacct ttgggtcagt ttcagccgaa
     3121 gctgttacat tttggtatat cggacatatt ggggctacaa tctgagcttt ttctattgcc
     3181 gcagccaatt ctactcgtgc tggctccatt aatggcgaat gaaaggcacc acctacaggt
     3241 aattttaagg ctctttttgc accagcttcc aacatgttgg cacacgcttc ttctaccgca
     3301 gctatagaac cagaaataac caattgtcca gggcaattat aattagcagc taccactacg
     3361 ccattgattg aagcacatat ttcttctact ttggcatcat caagtgccaa taccgcagcc
     3421 atggtcgatg gttcaatttc gcacgctttt tgcatagcca ttgcacgttt cgacaccaat
     3481 accaagccat cttcaaaact caatgcacca gcagccacaa gagcagaaaa ctctcccaac
     3541 gaatgacccg ctaccatatc gggagcaaat ttatcgccca tggttttggc taatataact
     3601 gaatgtaaaa ataccgcagg ttgcgtaact ttggtttgac gcaaatcctc atctgttcct
     3661 tcgaacatca aatcggtaat gcgaaaacct aatacttcgt ttgctttttc aaacatctct
     3721 ttcgctaaag ctgaattttc gtacatatct ttgcccattc ctacaaactg agctcctt
//
LOCUS       DAOPUR010000101         3764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24719_length_3764_cov_3.278781, whole genome shotgun sequence.
ACCESSION   DAOPUR010000101 DAOPUR010000000
VERSION     DAOPUR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24719_length_3764_cov_3.278781"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="PKN90_02550"
     CDS             complement(<1..1944)
                     /locus_tag="PKN90_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ85191.1"
                     /translation="MKKLIASFFLLLFAVICLAQQQQLPIIPTDPQVLIGQLPNGMTY
                     YIRKNSLPQERADFYIAQKVGSVIEEPNERGLAHFLEHMAFNGTTHFPNKDLLNYLQR
                     IGVKFGENVNAYTSVEQTVYLITNVPVVRETVIDSCLLILRDWANGITLSDTEIEKER
                     GVIEEEWRSRNSAWLRMAETILPTIYGNDKYADCLPIGHIDVIRNFKPDELRAFYKKW
                     YRPDLQAIIIVGDIDPQQVEAKLKATLGTIAMPSNPIPRPVYAVTDNQEPIIAFARDK
                     EATAISVEVSIKQDVFPTEMKGTIAYMMQEYVHAAVSGMMGSRFNEMIQKPNPPFTQA
                     YAYTDNFWVSNTKDARTLYAIASEQGATTAIKSLITENERMRQFGFTIAEYEREKANI
                     LKNIENQYNEREKQQNYEYVPKYINHFLENTPIPSIEYTFETYKQLAEMIPLELINNI
                     VKQASTTGNMVIVVQGPEDEKISFPTAEEILSGIEEVKRATIEPYTENVSNEPLISKL
                     PKPGKIKKEITHSNGTTDFILSNKVTVRLVPTNHKDDEILFQAYSHGGYSLVDKQYAT
                     TLKVLPELSTLSGLGNFSAIDLQKRLSGKNLRLSKQLNTYSESLNGYSGKKDVEMLFQ
                     LAYLHFTGIRSDQDAFQAYINRQS"
     gene            complement(1961..2317)
                     /locus_tag="PKN90_02555"
     CDS             complement(1961..2317)
                     /locus_tag="PKN90_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85192.1"
                     /translation="MRYILDDIDYVHTFQVYLGSWAPSFSYFVVMIVLLAASIFIIAE
                     NKLWIGWLLQLVGIIPWIYVFILNEYIYLLPEYQYEVFLIPASFMLWCSFAICIISLL
                     KITSILYTKHTNKQLQ"
     gene            complement(2400..3434)
                     /locus_tag="PKN90_02560"
     CDS             complement(2400..3434)
                     /locus_tag="PKN90_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85193.1"
                     /translation="MNKILHKLLYFTLLLLIFQLHTACNNKKQYDGRIEKEYTWTTLH
                     RFSVTNGYLVKAFINDTSITLSYNNKLAMYNQEGTLLQDTTLTYNGVLYQFIQEDSTF
                     WVDIFNGRQHLLQKFSNNNELLLDRKIDELSDILYVGNNKFIIPGYNKNSNTYRMELY
                     DAYNDTVINFINIGEITNLKDSITNAKGTITFTNKFATNKQGQVLAYYRYNSYFLLMD
                     ANLEYTAHQDFRKFPIGEPSYKGHNVLLNPLNCGIDKACIDDKFIYLLTPTYVCKQWN
                     KAKKDRMLDIYSLTTKAYLGSIRFPTIKNKEIVSIENTTKGFVTVYANDKETEIVTYN
                     NDFLSHFLYE"
     gene            complement(3444..3674)
                     /locus_tag="PKN90_02565"
     CDS             complement(3444..3674)
                     /locus_tag="PKN90_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ85194.1"
                     /translation="MAIIVNLDVMMAKRKISLNDLSEKVGITTANLSILKTGKAKAIR
                     FQTLEAICHVLDCQPGDILEFVDDDMSTNNEK"
BASE COUNT         1098 a          636 c          659 g         1371 t
ORIGIN      
        1 cgattggcgg ttgatatatg cctgaaatgc gtcttggtcg cttcgtatgc ctgtaaaatg
       61 taagtaagct aattgaaaca acatttctac atctttttta cccgaataac cgtttaatga
      121 ttctgaatac gtatttagtt gtttgcttag gcgaagattt ttgcctgaca atcttttttg
      181 taaatcaatg gcactaaaat tgcctaatcc actaagagta gaaagttcgg gcagtacctt
      241 taaggtggtt gcatactgtt tatctaccaa cgagtatcca ccgtggctat aagcttgaaa
      301 taatatttcg tcgtctttgt ggttggtagg aactaatcgt accgtaacct tgttggataa
      361 tataaagtca gttgtaccgt tgctgtgtgt aatctccttt ttaattttgc ctggtttggg
      421 taatttgctt attaatggct cgttcgatac gttttcggta tatggttcaa tcgttgcacg
      481 ttttacttct tcaattcctg ataagatttc ttcggctgta gggaaactaa ttttttcatc
      541 ttctggacct tgtaccacaa ttaccatatt gcctgtagta gaagcctgct ttacaatgtt
      601 atttatcaat tcaagaggaa tcatttctgc taattgttta tacgtttcaa aggtatattc
      661 aatgcttggt atgggcgtgt tttctaaaaa atgattgata tatttaggaa cgtattcata
      721 attttgttgt ttctctcttt cgttatattg attttctatg tttttaagaa tgttcgcttt
      781 ctcacgttcg tattcggcaa tggtaaaccc aaattgtctc atccgttcat tttcggtaat
      841 caacgattta atggcagttg tagcaccttg ttcagaagca atggcataca atgttcttgc
      901 gtcttttgta ttcgacaccc aaaagttatc tgtataggca taagcttgtg taaaaggcgg
      961 atttggtttt tgtatcattt cattaaaacg cgaccccatc attccgctta ccgcagcatg
     1021 tacatattct tgcatcatat acgcaatcgt tcctttcatt tcggtaggaa acacatcttg
     1081 tttaatggac acttctaccg atatggcagt tgcttcttta tcacgagcaa aagcgataat
     1141 aggttcttgg ttgtcggtta cagcatatac agggcgagga ataggattag aaggcatggc
     1201 tattgtacct agagttgctt ttagtttagc ctccacttgt tgagggtcga tatctccaac
     1261 aataataata gcttgtaaat caggtctata ccattttttg taaaaagctc ttagttcgtc
     1321 gggtttaaaa ttgcggataa catcaatatg tccaattggc aaacaatcgg catacttatc
     1381 gttgccataa atggtaggca aaatggtttc tgccatgcgt agccaagcac tatttctgct
     1441 tcgccattct tcttcaatta cacctctttc tttttcaatc tcggtatcag acagcgtaat
     1501 gccatttgcc caatcgcgga gtatcagtag gcacgaatct attactgttt cccttaccac
     1561 aggaacattg gtaatcagat atactgtttg ctcaacactg gtataagcgt tcacattttc
     1621 gccaaattta acgccaatgc gttgtaaata atttaataaa tccttgttgg ggaaatgggt
     1681 tgtgccatta aaagccatgt gttctaaaaa atgagccaat ccacgttcgt tgggttcttc
     1741 tataacagaa ccaacttttt gagcaatata aaagtcggct ctttcttgag gcaaactgtt
     1801 tttacgaata taatacgtca tcccattggg taattgtcca ataagcactt gtgggtcggt
     1861 aggaatgatg ggtaactgct gctgttgtgc caaacagata acagcaaaaa gtaatagaaa
     1921 aaatgatgca attagttttt tcattttggt gtagattaaa ttattgaagt tgtttattcg
     1981 tgtgttttgt gtagagaata gatgttattt ttagcaaact gatgatgcat atagcaaaag
     2041 agcaccatag cataaaagat gctggaatta aaaagacttc atattgatat tcgggcaata
     2101 aatagatata ctcattcaag ataaaaacat atatccacgg tataattccc actaattgca
     2161 agagccaacc aatccataat ttattttcgg caataataaa tatgctagca gccaatagta
     2221 caatcataac aacgaaatat gaaaacgaag gagcccaaga gcctaaatag acctgaaaag
     2281 tgtgaacgta atcgatgtcg tcaaggatgt atctcattat tgacgctatc gttaatagta
     2341 ttagaaataa tacattaaat atcaggggta taaccgttag aatggtgttt actattttat
     2401 tattcatata aaaagtggct taaaaaatca ttgttgtatg ttactatttc agtttcttta
     2461 tcgttggcat atacagttac aaatcctttt gtcgtatttt cgatacttac aatttcttta
     2521 ttctttattg ttggaaaacg aatgctacct agatacgctt tagttgtaag ggagtaaata
     2581 tcaagcattc tatctttttt tgctttattc cattgtttgc atacataagt tggcgttaac
     2641 aagtagataa atttgtcatc tatacaggct ttgtcaatac cgcaattgag cggattgagc
     2701 agtacattgt gccctttata tgaaggttct cctattggaa acttcctgaa atcttgatgt
     2761 gccgtatatt ctaagtttgc atccattaat agaaaataac tattgtagcg gtagtaggca
     2821 agtacttgtc cttgcttatt ggtagcaaat ttgttggtaa aagttattgt accttttgca
     2881 ttcgtaattg aatctttcag attcgttatt tccccaatat tgataaagtt tattactgta
     2941 tcattataag catcgtataa ttccattcta tacgtattac tatttttgtt atatccaggt
     3001 attataaatt tattatttcc aacatataga atatcgctta actcatctat ttttctatcg
     3061 agtaaaagtt cgttgttatt agaaaatttt tgcagcaaat gctgtctccc attaaagatg
     3121 tctacccaga atgtgctgtc ttcttgaata aattgatata aaacgccatt gtatgtgagt
     3181 gttgtatctt gcaataatgt accttcttga ttatacattg ctaatttgtt gttatagctc
     3241 aaagtaatgc ttgtgtcgtt aataaaagct tttaccaaat atccattagt aacagaaaaa
     3301 cgatgtaatg ttgtccatgt atattctttt tctattcttc catcatattg ctttttgttg
     3361 ttgcatgcag tatgtagctg aaatattagc aacagtaagg taaagtagag taatttgtgc
     3421 aaaatcttgt tcattatttt ttattatttt tcattgttgg tactcatatc atcatctaca
     3481 aactcgagta tatcaccagg ctggcaatcc aatacatggc atatcgcttc taaggtttga
     3541 aagcgtatag cttttgcttt tcctgttttt aaaatcgata aattagccgt agtaatgccc
     3601 actttttccg acaggtcgtt gagcgatatt tttcgctttg ccatcattac gtctaaattt
     3661 acaattattg ccatattaaa aaaaacaaat ttatgtataa gccaattata caatttttat
     3721 atcaattttt gtacttagct tttcttttat aaggtcagca tgtg
//
LOCUS       DAOPUR010000102         3760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24750_length_3760_cov_3.164912, whole genome shotgun sequence.
ACCESSION   DAOPUR010000102 DAOPUR010000000
VERSION     DAOPUR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24750_length_3760_cov_3.164912"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PKN90_02570"
     CDS             complement(<1..965)
                     /locus_tag="PKN90_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ85195.1"
                     /translation="MKEYIQNIVFSTIGKCADEMHQECYVIGGYVRDIFLQRPSKDID
                     VVVVGNGIELAERVCKIWGKRARLHIFKNFGTAQIKFQEMEIEFVGARKESYRLDSRK
                     PIVEDGTLVDDQNRRDFTINALAICLNQDRFGELVDPFNGVRDIENKILRTPLNPDIT
                     FSDDPLRMIRCIRFATQLKFHIEENTILALSRNKDRLTIVSKERIADEIQKIMQADKP
                     SIGWLLLDKCGLLPYILPELTAMKGVDTKSGLSHKDNFLHTLEVVDNIAKQTNNVWLR
                     WGALLHDIAKPITKKYVQGTGWTFYNHNYIGETMVARIFNQMKLP"
     gene            1181..1861
                     /locus_tag="PKN90_02575"
     CDS             1181..1861
                     /locus_tag="PKN90_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85196.1"
                     /translation="MAKRTLDSIHVLFPQEVEERKKALLKKYEIEELELERNFIFYDS
                     IIRVKQTVIDSIEHFFVVKADSFYRGERVYEHFMQATMFPKTSLKLDVTEKGTLNMSS
                     IYSCNFPLKYTTMLISSKGVFIQLEEQSSRLYTFRDGDTYWEIVNYNEKNVQDALSFI
                     NQFKQETIEITLQGQKKYSFILNNEDKNRIISTWYYATLLQEYNFAKTQHNIINSKIA
                     RLREKNKK"
     gene            1890..2327
                     /gene="trxA"
                     /locus_tag="PKN90_02580"
     CDS             1890..2327
                     /gene="trxA"
                     /locus_tag="PKN90_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNZ85197.1"
                     /translation="MIRLLRPFSSIFLLLITFSLSAQTKIGKQDFLQKIWNFEVHKND
                     LVLRSDLPVIIDFYADWCGPCRMLAPELEALQREYNGRIAIYKVNVDEERELAAIFNA
                     RSLPTIFFIPKDGTPTYVMGYRSRAELKQIVDNILLSKPRIQQ"
     gene            2440..>3760
                     /locus_tag="PKN90_02585"
     CDS             2440..>3760
                     /locus_tag="PKN90_02585"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNZ85198.1"
                     /translation="MSKTKYIFVTGGVTSSLGKGIISASLGKLLKARGYNVTIQKFDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNVQTTRENNITTGRIYQNVIK
                     KERRGDYLGKTVQVIPHITNEIKQNIKQLGTKSKFDFVITEIGGTVGDIESLPFIESV
                     RQLRWELGKDCLCIHLTYVPYVVAAKELKTKPTQHSVKQLLELGVQPDVLVLRTEHPL
                     SQGIKEKVALFCNVAKSAVIECRDVPSIYEVPLRMQEEGLDEIVLSKMGLEDVQPANL
                     TQWTSFLTKLQTATEIVRIGLVGKYVELPDAYKSIIESLIHASAYNERKLKLELIQSE
                     KLTAENVTKTLSGLDGILIAPGFGDRGIEGKFIACQYARENNIPTLGICLGMQCMVID
                     IARNMLGYTDANSTEFNERTSNNVIDMMEQQKGLSVKGGTMRLGAYDC"
BASE COUNT         1333 a          546 c          637 g         1244 t
ORIGIN      
        1 ataggtagct tcatttggtt aaaaatacga gctaccattg tttcgccaat atagttatga
       61 ttataaaaag tccaacctgt accttgcaca tatttttttg ttataggctt agcaatgtcg
      121 tgtaacagag caccccatct taaccataca ttattagttt gtttagcaat attatctact
      181 acctctagtg tatgtaaaaa gttgtcttta tgcgataatc ccgattttgt atcaacgcct
      241 ttcatggcag ttaattctgg taaaatataa ggtaataatc cacatttatc aagaagtaac
      301 cagccaatag aaggtttatc tgcttgcatg attttttgaa tttcgtctgc aattctttct
      361 ttcgacacaa ttgttaatcg gtctttattg cgagataaag ccaatattgt attttcttct
      421 atatggaact ttagttgtgt tgcgaagcga atacatctaa tcattcttaa tgggtcgtcg
      481 ctgaatgtta tatctggatt taatggtgtg cgtagaattt tattttcaat atcacgaaca
      541 ccattaaatg gatccactaa ttcaccaaaa cgatcttgat ttaaacatat agctaaggca
      601 ttaatagtaa aatctcttct gttctgatca tctactagcg tcccatcttc tacaatcggt
      661 ttgcgagaat ctaatcggta tgattctttt ctggctccta caaattcaat ttccatttct
      721 tgaaatttaa tttgagcagt tccaaagttc ttaaaaatat gtaatcgagc tctttttccc
      781 catattttac atactctttc agctaattca ataccattgc ccactactac tacatctata
      841 tctttagatg gacgttgcaa aaagatgtct ctgacataac caccaataac ataacactcc
      901 tgatgcatct catcagcaca ttttccaata gtcgaaaaaa caatattttg gatatattct
      961 ttcatttaaa tattattaac cctacaaaag tactttcttt acaccaatct gacaaaaaaa
     1021 tattattttt gcctttgatt tgaaggctat gaaaaaaaat atgtatgcgt ggtgtgttct
     1081 cctgtccatt ttgtttctat catgcaataa acaggaaaga tgtacaagtg ttcattattt
     1141 gcaaagagtt gatagcttat tgttattgaa caatattgag gtggcaaaac gaacactcga
     1201 ttcaatccat gtactatttc ctcaagaagt tgaggaaaga aaaaaggctc tgttgaaaaa
     1261 atatgaaatt gaagagttag aattagaacg taattttatc ttttatgata gtataatacg
     1321 tgtaaaacaa acagtaatag atagtataga gcattttttt gtagtaaaag ctgattcttt
     1381 ttacagaggt gaaagggtat atgaacattt tatgcaagca acgatgtttc caaaaacgag
     1441 cttgaaatta gatgttaccg aaaaaggtac attgaatatg agcagtatat attcgtgtaa
     1501 ttttccttta aaatacacta cgatgcttat ttctagtaaa ggtgttttta ttcaattgga
     1561 agaacaatca tcgcgtttgt atacatttag agatggagac acatattggg aaattgttaa
     1621 ttataacgaa aaaaatgtgc aggatgcact atctttcatc aatcaattta agcaagaaac
     1681 aatagaaata acgctacaag gacaaaaaaa atatagtttc attttaaata atgaagataa
     1741 aaataggatt atttctactt ggtattatgc tactttatta caagaataca attttgcaaa
     1801 aactcaacat aatattatta actctaagat tgcacgttta agagaaaaaa ataaaaaata
     1861 atttatttat tagtatataa taggacaata tgattagatt actgagaccc ttttcatcta
     1921 tatttttatt gttgataaca ttttcattat cagcacaaac taaaattggt aaacaagatt
     1981 ttttacagaa aatatggaat tttgaagtgc ataaaaatga tttagtatta cgttcagatc
     2041 tccctgtgat tattgatttt tatgctgatt ggtgtggtcc ttgtaggatg ctagctcctg
     2101 aattagaagc tcttcaaaga gaatacaatg gtcgcatagc tatttataaa gtaaatgtag
     2161 atgaagaaag agaactagct gcaattttta atgcacgatc tttacctact atatttttta
     2221 ttccgaaaga cggcacacct acgtatgtga tgggttatcg ttcaagagct gaattaaaac
     2281 aaattgtaga caatatattg ctgtctaaac ctagaataca gcaatagatt gagtgctaat
     2341 taaaagaaat ataaaaaata aaataggttt tgatttcaaa acctatttta ttttttgtaa
     2401 atttgcaccc attaaaaaac taatctaata gctataattg tgtcaaaaac gaagtatatt
     2461 tttgttacag gtggtgttac atcatcttta ggtaaaggaa tcatatctgc ttccttagga
     2521 aaactattaa aagccagagg ttacaatgta accattcaaa aatttgatcc atacataaat
     2581 attgatccag gtacattaaa tccatacgaa catggagaat gttatgtaac cgttgatggt
     2641 catgaagccg atttagattt gggtcattat gaacgatttt taaatgttca aactaccaga
     2701 gaaaataata ttacaactgg tagaatttat caaaatgtta taaaaaaaga acgtagagga
     2761 gactatctag gtaaaacagt tcaagttatc cctcatatta cgaatgagat taaacaaaat
     2821 attaaacagc ttggaacaaa aagtaaattt gattttgtta ttacggaaat cggaggtaca
     2881 gtaggagata tagaatctct accttttata gaaagtgttc gtcagttaag gtgggagtta
     2941 ggtaaggatt gtttatgtat acaccttacg tatgtgccat atgtggtagc agctaaggaa
     3001 ttaaaaacga aacctacaca gcattctgta aaacaactgc ttgaattagg tgtacaacca
     3061 gatgttttgg tgttacgaac cgaacatcct ttatcacaag ggattaaaga aaaagttgct
     3121 ttattttgta atgtagcaaa atctgctgta atagagtgta gggatgtacc gtcaatttat
     3181 gaagtaccat tgcgtatgca agaagaagga ttagatgaga ttgttctttc taaaatggga
     3241 ttggaggacg tacagcctgc taatttaaca caatggacaa gttttttaac aaaattacaa
     3301 accgctactg aaatagtgag aatagggcta gttggaaaat atgtagaatt gcctgatgcc
     3361 tataaatcta tcattgaatc attaatacat gcatcagcat ataacgaacg gaaactaaaa
     3421 ttagaactta ttcaatcgga aaaattgaca gcagaaaatg taactaaaac tttgagtgga
     3481 ttagatggta ttttgattgc tccaggtttt ggcgatagag gaatcgaagg taaatttatt
     3541 gcctgtcagt atgcacgtga aaataatatt cctacattgg gtatatgttt aggaatgcaa
     3601 tgtatggtaa tagatatagc aagaaatatg cttggctata cagacgctaa ttctactgaa
     3661 tttaacgaac gtacatcaaa caatgtaatt gatatgatgg agcaacaaaa aggattgtcc
     3721 gttaaagggg gaacaatgcg tttaggggca tatgattgtg
//
LOCUS       DAOPUR010000103         3744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_24896_length_3744_cov_3.440228, whole genome shotgun sequence.
ACCESSION   DAOPUR010000103 DAOPUR010000000
VERSION     DAOPUR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24896_length_3744_cov_3.440228"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKN90_02590"
     CDS             complement(<1..766)
                     /locus_tag="PKN90_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNZ85199.1"
                     /translation="MKVKLVLLSMMLFSLLVTTKAQIAPKREFRGVWMHTVFSDDYAQ
                     KSNEQIKNFLLQRLNEYQSVGMNVLIFQVRPEADAFYKSSYEPWSRFLTGKQGKAPAD
                     SFDPMTFLIDECHKRGMEFHAWLNPYRANANRKNELHPDHLYWKRKDLFVYFGNQLFF
                     NPGIPENRTHIAKVVKDIVARYDVDAIHMDDYFYPYPIKGSSFPDQKEFVKYGLQTGE
                     FKLYQKDDWRRNNVNLLIKELNTVIKKEKPWVKFGIS"
     gene            complement(770..1993)
                     /locus_tag="PKN90_02595"
     CDS             complement(770..1993)
                     /locus_tag="PKN90_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNZ85200.1"
                     /translation="MGAIYSLVIRIYGFLIYIAGFFSEKAKLLYKGQRNTLWLAENSI
                     DKNQPYIWVHAASLGEFEQGRPLIEAIKKDHPTYKIVLSFFSPSGYEVRKDYALADYV
                     CYLPLDTKANATRFIELIQPVKAIFIKYEFWPNYLQVLRKKGIETYIVSAIFRKEQIF
                     FSWYGGWYKKLLQCFTHIFVQDTSSFELLQTHGIHRVSIAGDTRFDRVATIAASPTQL
                     PIIESFKQDKKLIVLGSSWEPDEALLIPFVEKNDCKLIIAPHQIHTERIQSLLAKLSM
                     PTALYTQTTAEDVKHANCLVINTIGILSSVYRYADVAYIGGGFGVGIHNILEAAVFGV
                     PVLFGPNYHKFKEAVDLINEGGAFCIQTQTDIDSIGKNLLTDNAFYQQVCTQSASFVQ
                     HRIGATQGIMDQLFL"
     gene            complement(1993..3507)
                     /gene="gltX"
                     /locus_tag="PKN90_02600"
     CDS             complement(1993..3507)
                     /gene="gltX"
                     /locus_tag="PKN90_02600"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNZ85201.1"
                     /translation="MKQVRVRFAPSPTGPLHIGGVRTALYNYLFAKKHNGKFILRIED
                     TDTNRFVPGAEAYIIESFNWLGIQFDEGVSFGGNYGPYRQSERKEIYKLYVKELLDKQ
                     LAYIAFDTPEELDTKRAEISNFQYDASTRMQMCNALTLSANEVAQRIANGDNYVVRIK
                     IEPNQDIIVHDTIRGEVKINSSVLDDKVLYKSADELPTYHLANIVDDHLMEITHVIRG
                     EEWLPSAPLHVLLYRYFGWEDTMPQFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLQW
                     TDPKTGDTSAGYRENGYFPEAVVNFLALLGWNNGTEQELFSLDDLVHEFSLDRVSKSG
                     AKFDYEKGKWFNHTYLIHKTDTELARLFMPIVQAKGHAITEAKLTHIVGLLKERAHFV
                     ADLWEQAAFFFEAPTQYDDKAVQKRWKETTASMLAELAVFIQSLPNIDDSTHTEETVK
                     QWVETKGYGLGNVMNPLRLALVGELKGPHIFDITAILGKEETIKRIQKAIDTLG"
     gene            complement(3532..>3744)
                     /locus_tag="PKN90_02605"
     CDS             complement(3532..>3744)
                     /locus_tag="PKN90_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNZ85202.1"
                     /translation="TGNTLKTDMQQTIALTLAKKAAIKTGATLSHEEMNEIVQSVHQG
                     TIPAYTTDGKKILSTISFAEIDKTFN"
BASE COUNT         1009 a          715 c          762 g         1258 t
ORIGIN      
        1 gactaatgcc aaattttacc caaggttttt cttttttgat aaccgtattc agttctttaa
       61 tcagtaaatt gacattattt ctacgccagt cgtctttttg gtacagttta aattcccctg
      121 tttgtaagcc gtatttaaca aattcttttt ggtcgggaaa cgaacttcct ttaatcggat
      181 aaggataaaa ataatcgtcc atgtgtatcg catctacatc atagcgagct acaatgtctt
      241 ttactacttt ggctatatgg gtgcggtttt cgggtatacc gggattaaaa aacaattggt
      301 tgccaaaata cacaaataaa tctttgcgtt tccaatatag gtgatcgggg tgcagttcgt
      361 ttttgcggtt tgcattggca cgatatgggt tgagccatgc atgaaactcc atccctcttt
      421 tgtgacattc gtcaattaaa aaagtcattg ggtcgaaact atcggcagga gcttttcctt
      481 gtttgcccgt aagaaaacgg ctccatggtt cgtaagacga tttgtaaaaa gcgtcagctt
      541 cggggcgtac ttgaaatatc agtacattca tgcctaccga ttggtattcg ttgaggcgtt
      601 gaagtaagaa attttttatc tgttcgttgc ttttttgagc ataatcgtcg ctaaataccg
      661 tatgcatcca tacgcctcta aattcgcgtt taggggcaat ttgagcttta gttgtcacca
      721 atagagaaaa gagcatcatt gaaagtagta ctagttttac tttcatattt tataaaaata
      781 gttggtccat aataccttgt gttgctccta ttctgtgctg tacaaacgaa gcggattggg
      841 tacatacttg ttggtaaaag gcattgtctg tgagtagatt cttgccaatt gaatctatat
      901 ctgtttgcgt ttgtatacag aaagctccac cttcgtttat caagtcgaca gcttctttaa
      961 acttgtggta attcggacca aagagtacag gaacaccaaa tacagccgcc tctaagatat
     1021 tgtgaatacc tacgccaaac cctccaccaa tatacgctac atctgcatag cgatataccg
     1081 atgacaatat accaatggta ttgattacta aacaattggc atgttttaca tcctctgctg
     1141 tggtttgggt atagagagcc gttggcatag atagttttgc aagtaaactt tgtatgcgtt
     1201 cggtgtgaat ttgatgcggt gcaattatca gtttgcaatc gtttttctcc acaaacggta
     1261 taagtagggc ttcgtctggc tcccacgaac tacccaaaac gataagtttt ttatcttgtt
     1321 taaaagattc aataataggt agttgcgtag gtgaagctgc aatagtagct accctgtcaa
     1381 aacgggtgtc gccggcaata cttactcggt gtatgccatg agtttgaagt agctcaaaag
     1441 aactggtatc ttgtacaaag atatgcgtaa aacactgtaa tagttttttg taccagccac
     1501 cataccacga aaaaaagatt tgttctttgc gaaatattgc cgaaacaatg tatgtttcga
     1561 tgcctttttt gcgtaatacc tgcaagtaat tgggccaaaa ttcgtatttg ataaaaatcg
     1621 cttttactgg ttgtattagc tcaataaagc gtgtagcatt ggctttggta tcgagtggta
     1681 ggtagcatac ataatctgct agagcataat cttttcttac ttcgtagcca gagggggaaa
     1741 aaaaactaag tacgattttg tacgttggat ggtctttttt tatggcttcg ataaggggtc
     1801 ttccttgttc aaattcgccc aacgaagctg catgtaccca gatatagggc tgatttttgt
     1861 ctatactatt ttcagctagc caaagggtgt ttcgctgccc cttgtagagc agttttgctt
     1921 tttccgaaaa aaacccagct atataaatga gaaatccata tatgcggatt actagtgaat
     1981 atattgctcc cattagccta atgtatctat cgctttttgt attcgtttta ttgtctcttc
     2041 tttgcctagt atggcggtaa tatcaaatat atgaggacct tttagttcac caaccaaggc
     2101 taaacgcaag gggttcatta cattgccaag tccatatcct tttgtttcaa cccattgttt
     2161 tacagtttct tctgtatggg tactatcgtc aatgttgggt aaggattgaa taaaaacagc
     2221 taattcagcc aacatactgg cagtggtttc tttccaacgt ttttgcactg ctttgtcatc
     2281 gtactgtgta ggagcttcga aaaagaaagc cgcttgttcc cataagtcag ccacaaaatg
     2341 tgctcgttct tttagcaaac caacgatatg tgttagctta gcttcagtta ttgcatgtcc
     2401 tttagcttgt acaatcggca taaataacct tgctaattca gtatctgttt tgtggataag
     2461 gtaggtatga ttaaaccatt tccctttttc gtaatcaaat ttagcaccac ttttactcac
     2521 acggtccaac gaaaattcgt gtactaaatc atctaaacta aatagttctt gttctgtgcc
     2581 attattccat cctaatagtg ccaaaaagtt tacaactgct tctgggaaat agccattttc
     2641 gcggtatcca gccgatgtat ctcccgtttt ggggtctgtc cattgcaagg ggaataccgg
     2701 aaagcccaat cggtcgccat cgcgtttgct aagtttacca ttgccatcgg gtttcagcaa
     2761 taggggtaaa tgtgcaaatt gaggcatggt gtcttcccaa ccaaaatagc ggtagagcaa
     2821 tacgtgcaaa ggagctgaag gcaaccattc ttcgccacga atcacgtgcg ttatttccat
     2881 taaatggtca tctacaatat tggctaaatg gtaggtgggc agttcgtccg ccgatttgta
     2941 tagcacttta tcgtctaata ccgatgagtt aatttttact tcgccacgga tagtatcatg
     3001 tacaataatg tcttggtttg gttcaatttt gatacgaaca acgtaattat cgccattggc
     3061 tatgcgttga gccacttcat ttgccgataa ggtaagtgcg ttgcacatct gcatacgtgt
     3121 gctagcgtcg tattgaaagt tggaaatttc tgctcttttt gtgtcgagtt cttcgggcgt
     3181 atcaaaagca atatatgcca actgtttatc caatagttct tttacataga gtttataaat
     3241 ttctttgcgt tcgctttggc gatacggacc gtagttacct ccgaacgata caccttcgtc
     3301 aaattgaatg ccgagccagt tgaaagattc gataatatac gcttcagcac ctggcacaaa
     3361 gcggttggta tcggtatctt cgatacgtag gataaatttt ccgttgtgtt tttttgcaaa
     3421 taaataatta tacaaggctg tgcgtacacc gcctatgtgc aaaggacctg tgggagaggg
     3481 agcaaaacga actctaactt gtttcatgaa tcttgtatct aaatgtgttg tttaattaaa
     3541 cgttttatct atttcggcaa atgaaattgt tgatagtatt ttttttccat cagttgtata
     3601 tgcgggtatt gttccttgat gaacggattg tactatttca ttcatttctt cgtgcgacaa
     3661 tgttgcacct gttttaatag cggctttttt cgctaaggta agggctattg tttgttgcat
     3721 atctgtttta agagtattgc ctgt
//
LOCUS       DAOPUR010000104         3724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_25088_length_3724_cov_3.022349, whole genome shotgun sequence.
ACCESSION   DAOPUR010000104 DAOPUR010000000
VERSION     DAOPUR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25088_length_3724_cov_3.022349"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PKN90_02610"
     CDS             complement(<1..825)
                     /locus_tag="PKN90_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNZ85203.1"
                     /translation="MKNTFWLLIAISFLFASCKDESEDLGFLIQPSKDKITVVIDSFH
                     VATSNLFVDSIYARSSNFTLGKYTDNIYGSFNASFLTQFKYVQGATFPSNATADSLSL
                     VLYYKSFWGDSLSTHEVTVYALDELFNYEKNYYSNINVAEFTNKQTILGKKVYTAVDK
                     TIPDSIRNASDYVGSVTILLPNSLKEEFFTRSDIYTSQQAFLNFFKGIYATTTYGDGT
                     VLLIDSANIELKYSYATTTSADVDTIIKEKLIFPANKEITSVTHINHPTTLSTSITD"
     gene            complement(839..1654)
                     /locus_tag="PKN90_02615"
     CDS             complement(839..1654)
                     /locus_tag="PKN90_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNZ85204.1"
                     /translation="MDNVIKVLYVTQEIYPYLPESEIADICRKLPQAIQEKGKEIRTF
                     MPKFGCINERRNQLHEVIRLSGMNLIIDDTDHQLIIKVASIQAARMQVYFIDNDDYFN
                     RKSTTQDENGEDFDDNEDRAIFFARGVIETVKKLRWTPDIVHCHGWMTALMPIYIKKA
                     LHDDPFFSKAKVIYSIYDNGFSKHFSPQFAQKSILEGIEKTDLKSVQNDGSFDAVTRL
                     AIDFSDGVIAASENVNTELLEYAKKSQKMTLPYQSPENYVNSYNEFYDQIVGQ"
     gene            1919..2755
                     /gene="panC"
                     /locus_tag="PKN90_02620"
     CDS             1919..2755
                     /gene="panC"
                     /locus_tag="PKN90_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNZ85205.1"
                     /translation="MQIITTKSELCKSLTNSTGIRIGFVPTMGALHQGHLSLVMRSKR
                     ENEVTVVSIFVNPTQFNDSRDLEKYPRTLDADINLLSQVGCDIVFAPTVDEMYPEKDT
                     RIFNFGTLETVMEGRHRPGHFNGVAQIVSKLFDAVQPHIAYFGEKDFQQLAIIREMVK
                     QLNYTITIAGCPIVRESSGLALSSRNMLLSAEEHKKAPIIYQILSESCIFVAQKQPHE
                     IRNWVLNKFQNTGLDVEYFEIVDGYSLQPVSSWNESDYIVGCIAVFCGKIRLIDNIPF
                     KR"
     gene            2762..3112
                     /locus_tag="PKN90_02625"
     CDS             2762..3112
                     /locus_tag="PKN90_02625"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNZ85206.1"
                     /translation="MFIQVLKSKIHRVTITEANLEYIGSITIDEDLMEAANIIESEKV
                     HIVDNNNGERFETYVIKGERGSGTICVNGAAARRVQPGDTIIIMAYAFMDFEEAKQFK
                     PTCIYPDTRTNKLI"
     gene            3189..>3724
                     /locus_tag="PKN90_02630"
     CDS             3189..>3724
                     /locus_tag="PKN90_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ85207.1"
                     /translation="MPKLKTIYVCKNCGNDSPKWFGKCSACGEWNTCVEEIEHKESKS
                     ATRITGYDFQAQKPILLHEIEISQEKRIDMCNEEVNRVLGGGLVPGSMVLIGGEPGIG
                     KSTLVLQVALHLSNLRILYVSGEESAKQLKLRAERIASHQTDCYIVSETSLETIYKHI
                     QTIKPELLIIDSIQTIFT"
BASE COUNT         1251 a          552 c          668 g         1253 t
ORIGIN      
        1 atctgttatt gaagtagata aggtggtagg atgatttatg tgtgtaacac ttgtaatctc
       61 cttattggct ggaaaaataa gtttttcttt aataatagtg tcaacatcag cagaagtggt
      121 agttgcataa ctatatttta gttcaatatt agccgaatcg atgagtaata cagtaccatc
      181 tccataagtt gtagttgcat atattccctt gaaaaaattt aaaaaagctt gttggcttgt
      241 atatatgtca cttcttgtaa aaaactcctc ttttaaagaa tttggtagta aaatagttac
      301 acttcctaca taatcacttg cattacgaat ggagtctgga attgttttat ctacagcggt
      361 atatactttt ttcccaagaa tagtttgttt attggtaaac tcagctacat taatattaga
      421 ataataattt ttttcataat taaacaattc atccaatgcg tatacagtta cttcgtgagt
      481 agaaagggaa tcgccccaaa aacttttata atatagtacc aagcttaaag agtcggctgt
      541 tgcattagat gggaatgtag ctccttgcac atatttaaat tgtgttaaaa aagaagcgtt
      601 aaaactgcca tatatgttat cagtgtattt tcctaacgta aaattactgc ttctggcata
      661 tattgagtca acaaataagt tactagttgc tacatgaaaa gaatctatta caactgttat
      721 tttgtctttt gaaggttgga ttaaaaaacc caaatcttct gattcgtctt tgcatgaagc
      781 aaataaaaaa gagatagcta ttagtagcca gaatgtattt ttcatttttt gagaaaattt
      841 attgtcccac aatttggtcg taaaattcat tgtacgaatt tacgtaattt tctggagatt
      901 gatatggaag tgtcattttt tgtgattttt tagcatattc aagcaattct gtattcacat
      961 tttcacttgc agcaattaca ccatcagaaa aatctattgc aagacgagtt acagcgtcaa
     1021 aacttccatc attctgtacg gattttaaat ctgttttttc tatcccctct aatatagatt
     1081 tttgtgcaaa ttgtggacta aaatgtttgg aaaaaccatt gtcgtaaata gaataaatga
     1141 ctttagcttt agaaaagaat ggatcgtcat gcaatgcttt cttaatgtaa ataggcatta
     1201 atgcggtcat ccatccatgg caatgaacga tgtctggagt ccaacgcaat ttctttactg
     1261 tttcaattac acctcttgca aagaaaatag cccgatcctc attatcgtcg aaatcttcac
     1321 cgttttcgtc ttgagtcgta ctttttctgt taaaataatc atcattgtca ataaaataaa
     1381 cttgcatacg tgctgcctgt atagaggcaa ctttgataat gagttgatga tcagtgtcat
     1441 caataataag gttcattcct gatagtcgaa ttacctcatg tagttggttg cgtctttcat
     1501 taatacaacc aaatttgggc ataaatgtgc gaatttcttt gcctttttcc tgaattgctt
     1561 ggggcaactt acgacaaata tctgcaatct ctgattctgg taaatacggg taaatttcct
     1621 gcgtaacgta taaaacttta ataacgttgt ccattaaaaa tgaattagta atactcctcg
     1681 aaaaaaacaa tatgcaaaga tataaaaaaa atcgttaata agcaagaagt ttcccataaa
     1741 cccattctat tttgtaagta ttatttttag attggaaaca atcatattat catagcaaac
     1801 agaatgtgat tgtggtataa aaaagttgct ttttatatat tgatgataac ttatgtgggt
     1861 ttttcttttt catcccaaat attatttgtt cttttgcatc tcaattttga tgtaaatgat
     1921 gcaaattata actaccaaat ctgaattgtg taaatcatta accaattcta caggcattcg
     1981 tattggtttt gttcctacaa tgggagcact acatcaggga catttgtcgt tggtaatgcg
     2041 aagtaaaaga gaaaatgagg taactgtagt aagtattttt gttaatccta ctcagtttaa
     2101 tgatagcaga gacttagaaa agtatccgcg tacgttagat gctgatatta atctattatc
     2161 tcaagtaggg tgtgatatag tgtttgcccc tacagttgat gaaatgtatc cagaaaaaga
     2221 tactcgcatc tttaattttg gtacattaga aacggttatg gagggtaggc acaggcctgg
     2281 acattttaat ggagtggcac agattgtaag taaattattt gatgcagtac aacctcatat
     2341 tgcttatttt ggtgaaaagg attttcagca attagcaatt atacgtgaga tggtaaaaca
     2401 gttaaattat actattacga ttgctgggtg ccctattgtt agagaatcaa gtggtttggc
     2461 tttgagtagt cgaaatatgc tactttctgc tgaagaacac aaaaaagcac ctataatata
     2521 ccaaatatta tcagaaagtt gtatctttgt agcccaaaag caacctcacg agattcgcaa
     2581 ttgggtacta aataagtttc agaatacagg attagatgtt gagtattttg aaatagtaga
     2641 tggttactcc ttacaacctg tttcatcgtg gaatgaatcc gattatatcg ttggttgtat
     2701 tgcagtattt tgtggaaaaa taagactaat agataatata ccatttaaac gttaaaaaag
     2761 tatgtttata caggttttaa aatctaaaat acatcgggta acaattaccg aggcaaatct
     2821 tgaatacatt ggtagtataa ctattgatga agatttgatg gaagctgcta atattattga
     2881 gtcagaaaaa gttcatattg tggataataa caatggagaa cgctttgaaa catatgtaat
     2941 aaagggcgaa agaggtagtg gtacaatttg tgtaaatgga gctgctgctc gtcgagtaca
     3001 gcctggcgat acaatcatta tcatggcata tgcctttatg gattttgaag aagctaagca
     3061 gtttaaacct acttgtattt atccagacac tcgtacaaat aaattaattt gaaacaaaaa
     3121 gccacttttt aaatagagtg gcttttttat ttgtattttt tcttgtatct tgcaacaaat
     3181 ttgtatgtat gcctaaacta aaaactatat atgtttgtaa aaactgtgga aacgactcac
     3241 caaagtggtt tggtaagtgt tctgcatgtg gagaatggaa tacttgtgtg gaagaaatag
     3301 aacacaaaga gagtaaatca gccactcgca taacaggata tgactttcag gctcaaaagc
     3361 ctattttatt acacgaaata gagatttcac aagaaaaacg catagatatg tgtaatgagg
     3421 aagtcaacag ggtattagga ggaggattag tgccaggttc aatggtatta ataggaggtg
     3481 aacctgggat aggaaaatca accttggtat tgcaagttgc tctccatttg tcaaatctac
     3541 gtatattata tgtatctggg gaggaaagtg caaaacagtt aaaattgaga gcagaacgta
     3601 ttgcatcaca tcaaacagac tgctatatag taagtgaaac atctcttgaa actatttata
     3661 agcatattca aacaatcaaa ccagaactct tgattattga ttctattcaa accattttta
     3721 ctga
//
LOCUS       DAOPUR010000105         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_25163_length_3717_cov_3.286455, whole genome shotgun sequence.
ACCESSION   DAOPUR010000105 DAOPUR010000000
VERSION     DAOPUR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25163_length_3717_cov_3.286455"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKN90_02635"
     CDS             complement(<1..220)
                     /locus_tag="PKN90_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85208.1"
                     /translation="MLPQEIKHLIQRYKDKPLESVVFKKFPENVDVPLLLQQLEGRRF
                     IANKMPSWNTFIDELVFPCARRERERTFC"
     gene            complement(220..1686)
                     /locus_tag="PKN90_02640"
     CDS             complement(220..1686)
                     /locus_tag="PKN90_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNZ85209.1"
                     /translation="MKKIINIKIIIRTLGFLLLFQSFFMLLASFVSFLYNESDVQPIL
                     LSAGITLTVALIAILSISKPTEPIGKREAYLVVALVWVVFSLFGLLPFLLSGSIPSFT
                     DAFFETMSGFTTTGASILTDVESLSHGLLFWRSIIQWLGGMGIVVLSLAILPLLGMGG
                     MQLFVAEVPGPTKDKLHAKVSFTAKALWGIYVVLTASQALFLWLGGMDLFDGICHSFT
                     TMATGGFSTKNASIAYWNSPLIEYIIIVYMFLGGVNFSLYFSFLQGNFSKIFKNEELK
                     YYLSFVLVFTLLVVCSIVFYTPELVISVEQIIRHSLFQVVSLFTTTGFATYDYLLWSQ
                     FTWMLLIVTMLFGASAGSTSGGIKLVRIVLLLKNSYYEFKRLLHPNAIIPVRYNEKVV
                     MPQMMTNVLAFVALYITILVIGMIMLGAFGMNFDEAVGSAVTALSNVGPSLGSLGPSS
                     NFAAISLPAKWVMSFLMLIGRLELFTVLVILTPTFWSK"
     gene            complement(1695..3035)
                     /gene="trkA"
                     /locus_tag="PKN90_02645"
     CDS             complement(1695..3035)
                     /gene="trkA"
                     /locus_tag="PKN90_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNZ85210.1"
                     /translation="MRIIIAGAGEVGTHLAKLLSKENHTIILLDENGEKVKALEANYD
                     ILGIEGLPTSLKDLKEAQVENADLFVAVTPYESTNITACLLATNLGAKKTLARIDNYE
                     YLLPKNLEFFQRLGVDSLIYPEMLAAKEIVSALKRGWVRQYVEFSDGALVLLATKLRE
                     NAPIVNKTIEEAFDKENSTVRIIMIKRLGMDIIAKGKDKILAGDIVYFITTKDNIEQV
                     RIQAGKEDFEINNIMILGGGSRIGVRAIQELPRNKNIKVIEQNKEKSIRVIENTDNVL
                     VINGDGRDLELLKNEDIESYDAFVALTGNAETNILACLTAKNFGVKRTVAEVENLDYV
                     PLAENLNIGSIINKKSLAASHIYKLILGGNVSNVRCLTLADVEIVEFVAGEKSAIVKT
                     PIKDIRLPENVNIGGIIRDGVGFVAMGTSHIQPGDNVLVFCAEDTIRKVEKLFK"
     gene            complement(3057..>3717)
                     /locus_tag="PKN90_02650"
     CDS             complement(3057..>3717)
                     /locus_tag="PKN90_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ85211.1"
                     /translation="VALQNLNVVFCLDRAGLVGADGPTHHGNFDLAYFRVVPNLTIAS
                     PINEEELRNMMYTAQLPNKGPFVIRYPRGNGVLVDWQTPMKELPIGKGVCLKEGKDIA
                     VLSIGNIGNTVAKAIELLGSDSKKVAHYDMRFLKPIDEEMLHDIASKFKKVITVENGT
                     IKRGLGSAVLEFFADNNYLLSVTRLGLPDEFIQHGSNDELFKMLRLDDQGIAQSLRLL
                     V"
BASE COUNT         1293 a          757 c          520 g         1147 t
ORIGIN      
        1 ggcaaaaagt acgttctcgt tctcgtcgag cacaaggaaa tacgagctca tcgatgaatg
       61 tattccaaga aggcatttta ttagcaataa atctacgacc ttctaattgt tgcagtaaca
      121 atgggacatc tacattctct ggaaattttt taaaaacaac agattctaga ggtttatctt
      181 tgtaacgttg aataagatgc ttaatttctt gtggtagcat tatttactcc aaaatgtggg
      241 tgtaagaata accaaaactg taaataattc taaacgacca attaacatta aaaaagacat
      301 aacccatttg gcagggagag aaatagcggc aaaattagat gatggtccga gactacctaa
      361 actgggtcct acgttgctta atgcagtaac agccgaacct accgcctcat caaaattcat
      421 tccaaaagct ccaagcataa tcattccaat tactaatatg gtaatataaa gagcaacaaa
      481 agccaacacg tttgtcatca tttgaggcat aactactttt tcattataac gtactggaat
      541 aattgcattt gggtgcaaca aacgtttaaa ctcataataa ctatttttga gtaataacac
      601 aatgcgaacc aactttattc caccactagt tgatccagct gatgccccaa ataacatcgt
      661 aacaatcaac aacatccatg taaattgaga ccaaagtaaa taatcatagg tagcaaaacc
      721 tgttgttgtg aacaaagaaa ccacttgaaa taaactatgt cgaataatct gttcaacact
      781 tattactaac tcaggagtat aaaatacgat gctacaaact actaatagag taaatacaag
      841 aacaaacgaa agatagtact ttaattcttc atttttaaaa atctttgaaa aatttccctg
      901 taaaaaagaa aaatagagcg aaaaattaac tcctcccaaa aacatataca caatgattat
      961 atactcaatc aaaggagaat tccaataagc aatactggca tttttagtag aaaaacctcc
     1021 cgttgccatt gtcgtaaatg aatgacaaat accatcaaat aaatccatcc ccccaagcca
     1081 taaaaacaag gcttgcgatg ctgttaaaac tacataaata ccccacaatg ctttagctgt
     1141 aaaactaact ttagcatgca atttatcttt ggtaggtcct ggtacttctg caacaaaaag
     1201 ctgcattcca cccatgccca atagaggcaa aatagccaat gaaagcacta caatacccat
     1261 acctcccagc cattggataa tactccgcca aaacaataat ccatgagaaa gactttctac
     1321 atcggttaag atagatgctc ccgtagtggt aaaaccagac attgtttcaa aaaaagcatc
     1381 cgtgaaagaa ggaatggaac cactcaataa aaaaggaagc agcccaaaca atgaaaaaac
     1441 aacccaaact aatgctacta ctaaataagc ttctcgtttt ccaataggtt ctgttggttt
     1501 ggaaatagat aagatagcaa taagggcaac ggtcaaagta atgcctgcag acaataaaat
     1561 gggctgtaca tctgattcgt tatacaaaaa agaaacgaaa gatgccaaca acataaaaaa
     1621 gctttgaaac aataacaaaa agcctaatgt tcgaataatt attttgatat ttataatttt
     1681 tttcattaat tgagctactt aaataacttc tctacttttc taattgtatc ttcagcacaa
     1741 aaaacgagca cattatcacc aggttgaata tgagaagttc ccatggcaac aaagcctact
     1801 ccatctctaa ttatacctcc gatatttaca ttctcaggca aacgaatatc tttaattggg
     1861 gttttaacta tagccgattt ttctcctgct acaaactcta caatttctac atcagcaagg
     1921 gttaagcaac gtacattaga cacgttgcca ccaagaatta gtttatatat atgactggca
     1981 gctaatgatt ttttatttat aattgaccct atatttagat tttctgccaa aggtacataa
     2041 tctaagtttt cgacttcagc aaccgtacgt tttaccccaa aatttttggc tgttaaacat
     2101 gctaatatat ttgtttctgc attacccgtg agggcgacaa aagcatcata agactcaatg
     2161 tcttcgtttt ttaacaattc taaatcacgt ccatctccgt taataaccaa tacattatcg
     2221 gtattttcaa tgacacgtat acttttttct ttattttgtt caataacctt tatattttta
     2281 ttccttggca attcttgtat cgcacgtaca ccaatacgac ttcctcctcc caaaatcata
     2341 atattattaa tctcaaagtc ttcttttcct gcctgaatac gtacttgttc gatattatct
     2401 tttgttgtga taaaatatac aatatctcca gccaatattt tatcttttcc tttggcaata
     2461 atatccattc ctaatcgctt aatcataatg atacgcacgg tagaattctc tttatcaaaa
     2521 gcctcttcaa tagttttatt tacaataggg gcattttctc ttaatttggt agctaacaat
     2581 actaaagctc catcagaaaa ttcgacatat tgacgtaccc accctctttt gagtgcactt
     2641 acaatttctt tggctgccaa catctcagga tagataagag aatctactcc caaacgttga
     2701 aaaaactcca aatttttagg taatagatat tcatagttat ctatacgagc gagggtcttt
     2761 ttagctccta aattggttgc caaaagacaa gccgtaatat tagtactttc gtatggagta
     2821 acagcaacaa ataaatctgc attttctacc tgagcttctt tcaaatcttt taatgaggtt
     2881 ggcaaacctt caataccgag tatatcataa tttgcctcta aggcttttac tttttcacca
     2941 ttttcatcaa gcaaaataat cgtgtgattt tcttttgaaa gtaatttagc aagatgagta
     3001 cctacttctc ctgcaccagc aataataatt ctcataattg tattgtatga aatattttat
     3061 actaataaac gtaaactttg tgctattcct tgatcatcca aacgtagcat tttaaataat
     3121 tcatcattgg aaccatgttg aataaattca tcaggtaaac ccaagcgagt aactgacagt
     3181 aaataattat tatcagcaaa aaactctaaa acagctgacc ctaatcctct tttaatagta
     3241 ccattttcaa ctgtaatcac ttttttaaat ttgctagcaa tatcatgtag catttcttca
     3301 tctattggct taaggaatcg catatcatag tgtgccacct ttttgctatc acttccaagc
     3361 agttcaattg cttttgctac ggtatttcca atatttccaa tgctcaatac agcaatgtct
     3421 ttgccttctt tcaaacaaac accttttcca ataggtaatt ctttcatcgg ggtttgccaa
     3481 tcaactaata ccccattgcc acgtggataa cgaataacaa atggaccttt atttggcaat
     3541 tgtgctgtat acatcatgtt gcgtaattct tcttcgttta tgggagatgc aatagttaaa
     3601 ttaggaacca cccgaaagta tgctaaatca aaatttccat gatgagtggg tccatcagcc
     3661 ccaaccaatc cagccctatc caaacaaaat accacattca ggttttgcaa tgccaca
//
LOCUS       DAOPUR010000106         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_25197_length_3715_cov_2.681694, whole genome shotgun sequence.
ACCESSION   DAOPUR010000106 DAOPUR010000000
VERSION     DAOPUR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25197_length_3715_cov_2.681694"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PKN90_02655"
     CDS             <1..815
                     /locus_tag="PKN90_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85212.1"
                     /translation="IMSLLSLFILIVFFIESLGPLLPKQTITEYVEVQPFDSAESSIT
                     ESAIMRSNKLTLYEKFVNISFSTLIENKLLTFSFSNAHPDSICNSINSLVLDNELLMI
                     DTVIPDSIDSIQINLKDGTILNLNSYKKDEENYNKLLGYLPNNEFFICQESCSEASIT
                     YSSINLQNGQEFQGIPLFNNSTRTYYANVYFKDIIGDLYLIIKTWERNDDEFVTLYYK
                     EIPLYNYERDEELNYQISNIEWIADNILVFYFSESSGEKESVQVKMKILKSK"
     gene            complement(1092..1358)
                     /locus_tag="PKN90_02660"
     CDS             complement(1092..1358)
                     /locus_tag="PKN90_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ85213.1"
                     /translation="MKTYKFCQSCGYPLKKDTQGGGSEKDGGISKKYCSMCYDNGEFI
                     TPAEIDTAKKMQTFCIQEMKNAGINGFLAWLATRSIPTLERWKT"
     gene            complement(1464..>1722)
                     /locus_tag="PKN90_02665"
     CDS             complement(1464..>1722)
                     /locus_tag="PKN90_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNZ85214.1"
                     /translation="GPQGRELRLPLAFPDLNQRIENFEYKGKKITNYEMESSAIAGLA
                     ALMGHEAMTVCVVIANRMLKQFSKDYHPAVKKLVELVLERI"
     assembly_gap    1723..1732
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1733..2331)
                     /locus_tag="PKN90_02670"
     CDS             complement(<1733..2331)
                     /locus_tag="PKN90_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNZ85215.1"
                     /translation="MKKRYESSELIVNADKSIFHLHLHPEQIADKIILVGDLGRVDVI
                     SGYFDTIESEAQNREFKTVTGTYKGKQITVVATGIGTDNIDIVVNELDALANIDLDKR
                     EDKDAFRKLTFVRIGTCGGLQKNIPVDSFLVSEQSIGFDGVLNYYANRDSVCDLDFEQ
                     AFIQQTGYSSKLTAPYCIHSTPSLVDRIAQTDMLRGITIS"
     gene            complement(2454..3422)
                     /gene="floA"
                     /locus_tag="PKN90_02675"
     CDS             complement(2454..3422)
                     /gene="floA"
                     /locus_tag="PKN90_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839405.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNZ85216.1"
                     /translation="MIESGMLTFILIAVGFILLLIFFYYVPFLLWVSAKVSGVNVSLL
                     QLFLMRIRNVPPPILVRALIEGHKAGLEIKLDALEAHYLAGGHVERVVHVLVSASKAN
                     IDLSFQMATAIDLAGRDVFEAVQMSVNPKVIDTPPVAAVAKDGIQLIAKARVTVRASI
                     KQLVGGAGEETILARVGEGIVSSIGSNESHKAVLENPDTISKLVLKKGLDAGTAFEIL
                     SIDIADIDIGKNIGAQLQMDQAQADKNIAQAKAEERRAMAVALEQEMIAKAQEARAKV
                     IEAEAEVPKAMAQAFISGNLGIMDYYKMKNIEADTSMRENIAKPKK"
     gene            complement(3452..>3715)
                     /locus_tag="PKN90_02680"
     CDS             complement(3452..>3715)
                     /locus_tag="PKN90_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNZ85217.1"
                     /translation="IYKFMKSKTLDTISLHTTIEGKNNPLEGISVSVGDTGVAVSRLA
                     PMGKVRINGNTIEVKVVSDMIDTGEQVKVTEISTSGIVVERNT"
BASE COUNT         1127 a          719 c          600 g         1259 t
ORIGIN      
        1 ggataatgtc tttattgagc ctttttattt taatagtttt ctttatagaa agtttaggtc
       61 cacttcttcc aaaacaaaca attactgagt atgtagaagt tcaaccattt gacagtgctg
      121 aaagtagcat aactgaatcc gcaattatga gatcaaataa attgactctg tatgaaaaat
      181 ttgtgaatat ttcgttttct accttgattg aaaataaatt acttactttt tctttttcaa
      241 acgcacatcc agattcaatt tgtaatagta ttaattcatt ggttttagat aatgagttgt
      301 taatgataga tacagtaata cctgattcaa tagattcaat acaaatcaat cttaaagatg
      361 gaactattct taatctcaat tcttataaaa aggatgagga aaattataac aaactattgg
      421 gatatttgcc gaataatgaa ttttttattt gccaagagag ttgctccgaa gcgtctatta
      481 cttactcttc aatcaatttg caaaatggtc aagagttcca agggatacct ttgttcaata
      541 attcaactag gacatattat gccaatgtat attttaaaga cataatcggt gatttatatc
      601 ttattattaa gacatgggaa agaaatgatg atgaatttgt cactctttat tataaagaaa
      661 ttcctcttta taactatgaa agagacgaag agctaaatta tcaaatttcc aatatagaat
      721 ggattgctga caatattttg gttttttatt tctcagaatc aagtggagaa aaagaaagtg
      781 tacaggttaa aatgaaaata ttaaaaagca aataagtaac ttatttggcg caagtttcct
      841 aacttgtgcc cataattaag tgatcgttca gacaaatcat agatattttg gttttttgac
      901 cgagaaaaag ggacaatact aaatagcaag ttttcactgc tagctcaggt ctgataccaa
      961 tattactcaa aagaaagttt cttcattatt tcagtttacc acggttcagc tgacctcgcc
     1021 agcgaggcta caactcagca aacgtgtgtc aaaaaaagta tgtgagctgt aattgtttat
     1081 gtgcgcgagc atcaggtttt ccatctttcc aatgtaggaa tgctacgagt agctagccat
     1141 gcaagaaaac catttatgcc agcatttttc atttcttgta tgcaaaatgt ttgcattttt
     1201 ttagcagtat caatctcggc aggtgttata aattctccat tatcataaca catggagcaa
     1261 tatttcttac ttattcctcc gtctttctcc gaaccaccac cttgggtgtc tttctttaac
     1321 ggataaccac aactttggca aaacttatac gttttcatca cagtttattg aattacttat
     1381 actaacttcc ttcgataaca gaataaatac acacatgaac ccaatcatta ttacgtactt
     1441 aacagcacac agtttatgaa atcctatatc cgttccaaca ccaattctac caattttttt
     1501 acagcaggat gataatcttt actaaattgt ttgagcattc tgttggcaat tactacacat
     1561 acggtcatgg cttcgtgtcc catcaaggca gctaagccag caatagcaga actttccatt
     1621 tcgtaattgg taattttttt gcctttatac tcaaagttct caatgcgttg gtttaaatca
     1681 ggaaaagcca atggtaaacg aagttctctt ccttgtggtc cgnnnnnnnn nngaaatagt
     1741 tatacctcgc aacatgtctg tttgtgcaat acggtcaacc aatgaaggag tggaatgaat
     1801 acagtagggt gcagttagct tactcgaata gcccgtttgc tgaataaatg cttgttcaaa
     1861 atctaagtcg catacactat ctcggtttgc ataataatta aggactccat caaagccaat
     1921 agattgttca gacactaaaa aactatctac aggtatgttt ttttgcagac cgccgcaagt
     1981 accaatacga acaaatgtaa gtttgcgaaa agcatctttg tcttctcttt tatctaaatc
     2041 gatattagcc aaagcatcta attcatttac tactatatca atgttgtcgg taccaattcc
     2101 tgtggctact accgttattt gttttccctt gtaggtgccg gtaacggttt taaattcacg
     2161 gttttgtgct tcgctttcta tggtgtcaaa atagccactt atcacatcta cacgtcccaa
     2221 atctcctacg agaattattt tgtcagcaat ttgttctgga tgcaaatgta agtggaagat
     2281 gcttttatca gcgtttacaa tcaattctga actttcatat ctttttttca tagttggtgc
     2341 ttttattgat taaatagtaa acaaatactc actcattttt cgatcactga tttacttcta
     2401 gtgccaaaaa caagtgagta tgtaactcta agagaagaaa taagtacgaa tgtttatttc
     2461 tttggtttag ctatgttttc gcgcatagaa gtatcagctt cgatgttttt cattttataa
     2521 taatccataa tacccaagtt gccactaata aacgcttgag ccatagcttt tggcacttct
     2581 gcctctgctt caattacttt ggcacgtgct tcttgtgctt tggctatcat ttcttgctca
     2641 agggcaaccg ccatagctct acgctcttcg gctttggctt gtgcaatgtt tttatctgct
     2701 tgtgcttggt ccatttgcaa ttgagcccca atgtttttac ctatgtcaat atcagcgata
     2761 tcaatggata aaatttcaaa cgccgtaccc gcatctaatc ccttttttaa tacgagtttt
     2821 gaaatcgtat ctggattttc taatactgct ttatgcgatt cgttagaacc gatagaagat
     2881 acaattcctt ctcctacacg agctaaaata gtttcttcgc cagctccacc cactaattgt
     2941 ttgatacttg cacgtaccgt tacgcgggct ttagcaatta actgaatacc atctttagct
     3001 actgcagcta ctggtggggt gtcaattact ttgggattaa ctgacatctg tacagcttca
     3061 aatacatctc tacctgcaag gtcaatagcc gttgccatct gaaatgacaa atcgatattc
     3121 gccttagacg ccgaaaccaa tacatgcact acacgctcca catgtccacc tgctaaatag
     3181 tgggcttcta acgcatctaa tttaatctct aacccagctt tgtgaccttc gatgagtgct
     3241 ctaaccaata ttggaggtgg cacatttcga atacgcatca aaaacagctg aagtaacgat
     3301 acatttacgc ccgatacctt ggctgatacc catagcaaaa acggaacgta gtaaaaaaag
     3361 attaataaca gtataaatcc tactgctatc aaaataaagg ttaacatacc actttcaatc
     3421 atagtttttt atttgttaaa ttaatagttt tttatgtatt gcgttctaca acgatgcccg
     3481 acgtagaaat ttcagttact tttacttgtt cgcctgtatc tatcatgtcc gataccactt
     3541 ttacttctat tgtattgccg ttaattcgca ctttgcccat aggggctagt cgcgatacag
     3601 ccacccccgt atctcctact gatacagaaa taccttctaa tggattattt tttccctcaa
     3661 tcgttgtatg tagggagatg gtatccaatg ttttggattt cataaattta taaat
//
LOCUS       DAOPUR010000107         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_25377_length_3693_cov_3.401594, whole genome shotgun sequence.
ACCESSION   DAOPUR010000107 DAOPUR010000000
VERSION     DAOPUR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25377_length_3693_cov_3.401594"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..503)
                     /locus_tag="PKN90_02685"
     CDS             complement(108..503)
                     /locus_tag="PKN90_02685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNZ85218.1"
                     /translation="MASIFSKIVQGEIPCHKIAETPDYLAFLDVRPLVRGHVLVIPKQ
                     EVSYIFDLEDNVLSGLHVFAKKIAKAIEKAIPCKRVGVAVIGLEVPHAHIHLIPLTKE
                     SDIYFSNTKLELTDDELASIATSIRTFLA"
     gene            complement(515..985)
                     /gene="greA"
                     /locus_tag="PKN90_02690"
     CDS             complement(515..985)
                     /gene="greA"
                     /locus_tag="PKN90_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNZ85219.1"
                     /translation="MSITYMTKEGYKKMMDELNHLESVERPNISMQIAEARDKGDLSE
                     NAEYDAAKEAQGMLELKIAQLKETVKNARIISEKDLNTDYVQILNKVRIRNLKNNQEL
                     TYTLVSEQEASLKEGKIAINTPVAKALLGKKVGEIVEVTVPSGVLRLEIINISF"
     gene            complement(1156..1455)
                     /locus_tag="PKN90_02695"
     CDS             complement(1156..1455)
                     /locus_tag="PKN90_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85220.1"
                     /translation="MGSKKEVVAQVPPIVHKPKPFLAENKNKQPHVNNTKSSQATNLN
                     NTSFSDMSTISEKKLPRDEKLHNVVEISTDNDFTLHQEDLKKAIVYAEIINRKYK"
     gene            1830..3158
                     /gene="miaB"
                     /locus_tag="PKN90_02700"
     CDS             1830..3158
                     /gene="miaB"
                     /locus_tag="PKN90_02700"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNZ85221.1"
                     /translation="MKNQKKLFIETYGCQMNVADSEVVASIMGMSGYELTDNINEANA
                     IFINTCSIRDNAEQKAFSRLDYFQSLKKKRKDIIIGVLGCMAERIKEDLLERKVIDMV
                     VGPDAYLDLPNLIGAVESGEKAINIELSTTETYQDIIPQRIGHNRISGFVSIMRGCNN
                     FCSYCIVPYTRGRERSRPAASIINEMKDLVAKGYKEITFLGQNVNSYLDSSEDNKYTF
                     PDLLAMAANTFPDTRIRFTTSHPKDISEELIRTIATYHNLCKHIHLPVQSGSNRILSL
                     MNRKYTREEYLQKIAMIKRIIPECGITTDVFTGFHSETEADHQETLSLMQEVGFDNAF
                     MFKYSERSGTYAAKHLEDNVPEAIKLSRLQEVVDLQNKLSKESNQKDLGKIVEVLIEG
                     YSKKSRNQLFGKSSENKVVVFNKENKKIGEYAKIQIKDATVATLLGEICE"
     gene            3264..>3693
                     /locus_tag="PKN90_02705"
     CDS             3264..>3693
                     /locus_tag="PKN90_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85222.1"
                     /translation="MKKLFTFLIVSVLTITSLSAADYTHWSVYIDGGASQFDGDVSQR
                     YNQINPGADIHWTAGLGVEYTFTPFWGLGVEYQYMPYSGYSTSTGQGWWSNYGEFDFE
                     GGSHQAHIFTSLNLTNLLNVYRKTWRFNVYANIGLGASFYN"
BASE COUNT         1274 a          643 c          588 g         1188 t
ORIGIN      
        1 agacgtacgt attacaaaaa agccgatctc actattaatg tggttgaaaa ttagaagagg
       61 aaagtcttat aaactctcct cctctgtcaa ttatatgtta gctcccttta ggcaagaaat
      121 gttctaatgg aagtggcaat agaagctaat tcatcatctg taagctctaa ttttgtatta
      181 gaaaaataga tatctgattc ttttgtcaac ggaattaaat gaatatgagc atgtggcact
      241 tctaaaccaa ttacagcaac accaaccctt ttgcatggaa tagccttttc aatagctttt
      301 gcaatttttt ttgcaaatac atgtaaccca ctcagcacat tatcttccaa atcaaaaata
      361 tacgacactt cctgttttgg aatcaccaat acatgccccc tcactaacgg acgaacatct
      421 aaaaatgcta gataatctgg ggtttctgct atcttatgac aaggaatttc tccttgaaca
      481 attttactaa aaatactagc catagtatat ctttttaaaa agaaatattt attatttcca
      541 atcgtaatac accagaagga actgttactt cgactatctc tcctactttt tttcctaata
      601 atgccttagc cactggagtg ttaatcgcta tctttccttc tttcaaactt gcttcttgct
      661 cagacacaag agtataagta agctcctggt tatttttcaa attacgtata cgtactttgt
      721 ttaaaatctg aacataatct gtatttaaat ctttttcact gataatacga gcatttttca
      781 ctgtttcttt tagctgagca attttcaatt ccagcattcc ttgagcctct tttgccgcat
      841 catattcagc attttcagac aagtctccct tgtcacgagc ctctgcaatt tgcatcgaaa
      901 tgttagggcg ttccaccgat tccaaatgat ttagttcatc catcattttc ttgtaccctt
      961 ctttggtcat gtacgtaata ctcataataa aataatttag attaaaaaaa tagtccttta
     1021 acgtatttac ataaataaag agaatctcga aattaatcga gactctctgt tgtgtttgta
     1081 attacttcgg caaagataaa gctttctttt caaagaaaaa aatatttcat cgaataaatc
     1141 tttgagctaa gaattctatt tgtacttcct gttgataatt tcggcataca caattgcctt
     1201 tttcaaatcc tcctgatgta aggtaaaatc attatcagta ctaatttcga caacattatg
     1261 tagtttttca tctcgaggca gttttttttc tgatatcgtt gacatatcag aaaaacttgt
     1321 gttgtttaaa ttagtcgctt gggaagattt agtgttatta acatgtggtt gtttgttttt
     1381 attttcagcc aaaaatggtt ttggcttatg cacaatagga gggacctgtg ctactacttc
     1441 ttttttactc cccaacttac gtattatgaa tagaataaaa acaacgataa aaataatggt
     1501 ggatattgac attgtatttg tgtataatga atgtgtacta caaagataat aaaaaattaa
     1561 ctagatacca aaaattaaaa ggagagccgc tgcccccctt ttaacctaac ctaaacctta
     1621 actatgaaaa aaaatcattg tttctttttc ttgatacaaa gaaaagcatt tgttttgaat
     1681 tacgcaaacg tttgcatagt ttctaacgca aacgtttgcg tttttaacct tatttaacgt
     1741 ttattcgttg ttttagctaa atattccaag aatattcact ataaaaaata aaaaacatac
     1801 ctttgtaccc taattatttc ggacataaaa tgaaaaacca aaaaaaatta tttatagaaa
     1861 catacggatg tcaaatgaac gttgcagaca gcgaagttgt agcatcaatt atgggcatga
     1921 gtggatacga gctcacagat aatataaacg aagcaaatgc catcttcatt aacacctgtt
     1981 ccatcagaga caatgcagaa caaaaagctt tttcacgcct tgattacttt caatcgttaa
     2041 aaaagaaaag aaaagatatc attattggag tgttaggctg tatggctgaa agaattaaag
     2101 aagatttact tgaacgaaaa gtcatagaca tggttgttgg tccagatgcg tatcttgatt
     2161 tacctaatct gatcggagcg gtagaatctg gagagaaagc tataaatatt gaactttcga
     2221 caaccgaaac atatcaagat attataccac agcgtattgg acataatcga attagtgggt
     2281 ttgtgtccat catgcgagga tgtaataatt tttgttctta ttgcattgtt ccgtacacac
     2341 gaggcagaga aagaagtcgc cctgctgcaa gtattattaa cgaaatgaaa gatttagtag
     2401 ccaaaggtta caaagaaata acattcttag gtcaaaatgt aaattcatac cttgattcat
     2461 ctgaagacaa caagtacacg tttccagatt tacttgcaat ggctgcaaac acattccctg
     2521 atacaagaat ccgcttcacc acttctcacc caaaagatat tagcgaagaa ttaattcgca
     2581 ctattgccac ataccataat ttatgcaaac acattcatct acctgtacaa tcaggtagta
     2641 atcgtatatt gtccttaatg aatagaaaat atactcgtga agaatatttg caaaaaattg
     2701 ctatgataaa acgcattatc ccagaatgtg gtataaccac tgatgttttt acaggattcc
     2761 attctgaaac agaagccgac catcaagaaa cgctttcact tatgcaagaa gttggatttg
     2821 acaacgcctt tatgtttaaa tactccgaac gaagtggcac atacgcagct aaacacctcg
     2881 aagataatgt tccagaggca atcaaattat cgagactgca agaggttgtt gatttacaaa
     2941 acaaactttc caaagaaagt aaccaaaaag atcttggaaa aattgttgaa gttttaatcg
     3001 agggatattc aaaaaaatca cgcaatcagc tttttggaaa atcctcggaa aataaagtcg
     3061 tcgtatttaa taaagaaaat aaaaaaattg gcgaatacgc caaaatacaa attaaagatg
     3121 ccacggtagc aacactttta ggtgaaattt gcgagtaata gtctaaaaat taggcaaaaa
     3181 tgaaaacttt attgaaaaaa aagaaaaaaa taaaaatatt gtttttatat ttgtcccctg
     3241 aataattaac taaaacacgt tatatgaaaa agttatttac atttttgata gtttctgtgc
     3301 taacgataac ttcgttatca gctgcagact acactcattg gtctgtttat atcgatggag
     3361 gagcttctca gtttgacgga gacgtttcac aaagatacaa tcaaataaac cctggagcag
     3421 atatccattg gacagctgga ttaggtgttg aatatacatt tactccattt tggggattgg
     3481 gcgtagaata ccaatatatg ccttattctg gttactcaac ttcaacaggt caaggatggt
     3541 ggtctaatta cggagaattt gactttgaag gaggatcgca tcaagcacat atatttactt
     3601 ctttaaatct aactaattta ttaaatgtgt atagaaaaac atggaggttt aatgtctatg
     3661 caaacattgg tttaggtgct tctttctata atg
//
LOCUS       DAOPUR010000108         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_25976_length_3632_cov_3.589321, whole genome shotgun sequence.
ACCESSION   DAOPUR010000108 DAOPUR010000000
VERSION     DAOPUR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25976_length_3632_cov_3.589321"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKN90_02710"
     CDS             complement(<1..495)
                     /locus_tag="PKN90_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85223.1"
                     /translation="MKKFAILLVIFVSSLFTSHLYAQSCTTQFSGTTFQPKTGITSAN
                     YNMDQIRLEDPSSEVLTTGMTFKNSNTLDRGEYTISNNTIKIATKTASQYGILSYALS
                     NLMTTAGQNQYRVEITVKTYPNPGCSPTMESHRGVNYKFSVGNYNIYHEVKDETYTFG
                     YTFTA"
     assembly_gap    885..894
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1107..2384)
                     /locus_tag="PKN90_02715"
     CDS             complement(1107..2384)
                     /locus_tag="PKN90_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNZ85224.1"
                     /translation="MNKLAHCIFLAIFVTHTLYSCTTNEPTSPPQPKEEYITMDINKS
                     SPKAFYYSIDDALENAETPEKKLKLGDSIYLLAKEKQWIPIVFADTAVFLYRHSSTNN
                     ATVEVFGDLNGWSTSESPQISLSRYADTRLYAGIYKAPSTTTRVDYKLVVNNAWILDP
                     GNPKLAWGGMGSNSEVALSEYEYSPWVKARSDINKGTLSANQLITSSALGYNINYRVY
                     LPYGYDSELSYPVLFVTDGHEYINNNLGALPNVADNMLHENKIEPLIIVFVDPRDPTN
                     GTNKRMTEYVANDNFVTFLADELYTTIAQTYSIKNTPEHTAILGTSLGGLNSAYVGIK
                     RADVFGLLAIQSPAFWIHTDIFTLFNGDKQNLRIYMDTGTIHDTQDRALEMKTILENK
                     GYTFQYAEYSEGHSWGNWRARMDDVLLYFWGKE"
     gene            2570..3265
                     /locus_tag="PKN90_02720"
     CDS             2570..3265
                     /locus_tag="PKN90_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ85225.1"
                     /translation="MRKTHLLIGFLSIAIIFSGCKSWSNTAKGGLIGGGAGAAIGAGV
                     GILIGKNEKSAAIGAAVGTAVGATTGLIIGKQMDKAAEKAAAIEGAKVDTITDANNLK
                     AVKVTFESGILFASGKSILSTKSKTSLSDFAAILVENPTMDVAIMGHTDNTGSLELNK
                     KLSQERAQAVADFLVSKNVPTKQIKEVVGKDFSEPVADNATVEGKAANRRVEIYMYAS
                     EEMIKNAENAVKK"
     gene            complement(3420..>3632)
                     /locus_tag="PKN90_02725"
     CDS             complement(3420..>3632)
                     /locus_tag="PKN90_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNZ85226.1"
                     /translation="TTEKKIIGYGSISTLESTLQAFNFIRIHRSYLVAKNKIGTIATN
                     SIIVSTYELPIGRNYRKNIENIAKLS"
BASE COUNT         1140 a          564 c          731 g         1187 t
ORIGIN      
        1 agccgtaaaa gtatatccaa aagtgtatgt ttcgtctttt acttcgtgat aaatgttata
       61 gtttcctaca ctaaatttat agttaacgcc acgatgagat tccatagtag gactacaacc
      121 aggatttggg taagttttta cagtaatttc aactctgtat tgattttgtc ctgcagttgt
      181 cattaagttg ctcaaagcat aagaaagaat tccatattgg gaagctgttt ttgtagcaat
      241 tttgattgta ttattactga tagtatattc ccctctatca agtgtatttg agtttttaaa
      301 agtcatacca gtggttaaaa cttctgagga tggatcttct aaacggattt gatccatgtt
      361 gtaattggca gaagttatac ctgtttttgg ttgaaacgtg gtgccactaa actgagttgt
      421 acaactttgg gcatacagat gagaggtaaa caaagaggat acaaaaataa cgagcaaaat
      481 agcaaacttt ttcatagcaa gtctatatta aatataaggt tattaaggct tattcacaca
      541 aaaagccctc aaaaatacaa aatctattct gaaataaaaa ataatagtta agtatttaac
      601 taaaaaaatg ctatttttat tgtgtaaggg gaggagcatg tttagtgatt agtgtttgtt
      661 gattccttat ttgcggtatt ttgttaggtt cttgcacggt tttgttaata attgttacac
      721 agagttctac ggaggaatca cagagtttat aatttaaaaa aatagattgc tatcagcata
      781 ttattatgtc gctcctacgg agctggtgtt ttgccagttt taaggaaaaa tgcaactttt
      841 catgccccgt aggggctaca tcataataga aaagaaagat gccannnnnn nnnngctaat
      901 attaagttat tagctccagc ggagcggaat agatgtcttc gtgttcttcg tgcgagaatc
      961 tttcacagag ttttacggag gaatcacaga ggggcacaga ggatttagtt ttttcttcgt
     1021 gtaacttcgt gttcttcgtg tgagaaatag tacacagggt tccaaactct caactctcaa
     1081 ctttcaactc tcaactaatt taactcctac tctttacccc aaaaatacag caacacatca
     1141 tccattcgtg ctcgccaatt gccccacgaa tgtccttcgc tgtattcggc atactgaaat
     1201 gtatagcctt tattttcaag aattgtcttc atctctaatg ccctatcttg cgtgtcgtga
     1261 atggttccag tatccatata aatacgtaag ttttgtttat cgccgttgaa tagcgtaaaa
     1321 atatcggtat gtatccaaaa tgcaggcgac tgtatggcta gtaatccaaa tacatcagca
     1381 cgtttaatgc caacataagc actattaagc cctcccaacg aagtgcctag tatggcggtg
     1441 tgttcaggag tatttttaat gctatatgtt tgagcaatgg tagtatataa ttcatctgca
     1501 aggaatgtta caaaattatc attggcaaca tattcagtca ttcgtttgtt cgtgccgttg
     1561 gttgggtcgc gtggatcaac aaatacaata ataagaggtt ctattttatt ttcgtgtagc
     1621 atattgtcag ctacattggg taatgcaccc aaattattgt ttatgtattc atgtccgtct
     1681 gtaacaaaaa ggacggggta agacaactcc gaatcgtatc cataaggcaa atatacacgg
     1741 tagttgatgt tgtaccccaa agctgaactg gtaatcagtt ggttagcaga aagagttcct
     1801 ttgtttatat ccgaacgtgc ttttacccaa ggagaatatt cgtattcgct gagggctact
     1861 tctgaatttg accccattcc gccccaagct aatttagggt tgcctgggtc tagtatccat
     1921 gcgttgttta caactagttt ataatcaacg cgggtagtag tagagggggc tttataaatg
     1981 ccagcgtata ggcgagtatc agcatagcga gaaagtgata tttgagggga ttcagaggta
     2041 ctccacccat ttaaatcgcc aaaaacttcc actgtggcgt tgtttgtact cgaatggcga
     2101 tacaaaaaca cagccgtatc agcaaacaca atcggtatcc attgtttttc tttggcaagc
     2161 aagtaaatgc tatcgccaag tttcagtttc ttttcgggtg tttcagcgtt ttctaaagca
     2221 tcgtcgatag aataataaaa tgcttttggg ctagacttat taatatccat ggttatatat
     2281 tcctcttttg gttgaggggg agatgttggt tcgtttgtag tacacgaata cagggtatga
     2341 gtaacaaaaa tggcaagaaa aatacaatga gctaatttgt tcatagtaca cttaatgtaa
     2401 agttcgttta tgtttcaaaa ataaggaata tattttgata aaccaaatat attcataaaa
     2461 gtatcttttt aagatttttt gccacataga attttttaga tatagctact ttttgtatct
     2521 ttgtggagat tttaataaaa caagtaattt acaaaaaacg tttaacaaaa tgagaaaaac
     2581 acatttactt attgggtttt tgagtattgc tatcattttt tcgggatgca aatcgtggag
     2641 caataccgct aaaggtggat tgattggagg aggtgctggt gctgcaatag gtgcaggagt
     2701 tggtattttg attggtaaaa atgagaaaag tgcagcaatt ggtgcagcag taggtacagc
     2761 agtaggagct actacagggc ttattattgg gaaacaaatg gacaaagctg cagaaaaagc
     2821 cgcagctatt gagggtgcta aagtagacac gattaccgat gctaataact taaaagcagt
     2881 aaaagttacg tttgaatcgg gtattttgtt tgctagtggc aaaagtattc ttagtactaa
     2941 atcaaaaacc tcattgagtg attttgctgc tattttagta gaaaatccta ctatggatgt
     3001 agctattatg ggacatacag acaatactgg ttcattggag ttgaataaaa aattatcgca
     3061 agaaagagca caagctgtag ctgatttttt ggtgtcaaaa aatgttccaa caaaacaaat
     3121 caaagaagta gttggtaaag atttttctga gccagtagct gataatgcta ctgtagaagg
     3181 taaagcagcc aatcgtcgtg tagaaatcta tatgtatgcg agcgaagaga tgattaaaaa
     3241 tgcagaaaat gcagtaaaaa aataacattc ttttgtttag ggagaaagag tttgctacat
     3301 agcaaactct ttttttgtat tagtataagt agtttcagtt tacagtgcgt tgtaaaatat
     3361 aatactggta agggtctaaa gataagcttg tttcgagttc tatattttgt tttgcatagt
     3421 tatgataatt tagcaatatt ttctatattt tttcgataat tgcgaccaat tggcagttcg
     3481 taagtagata caataataga gtttgtagca atggttccaa ttttattttt agctactaaa
     3541 tatgaccgat gaatgcgtat aaaattaaaa gcttgaagag ttgactccag tgttgaaata
     3601 gaaccgtatc caattatttt tttctctgtc gt
//
LOCUS       DAOPUR010000109         3628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_26019_length_3628_cov_3.893927, whole genome shotgun sequence.
ACCESSION   DAOPUR010000109 DAOPUR010000000
VERSION     DAOPUR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26019_length_3628_cov_3.893927"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKN90_02730"
     CDS             complement(<1..202)
                     /locus_tag="PKN90_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85227.1"
                     /translation="MQHIKPYILFFVFLLVFFSCTKEKTIDTPPTHETNSITQTNVLE
                     KMLGTYSLFDRNWNLMEYTIFQE"
     gene            complement(207..944)
                     /gene="pssA"
                     /locus_tag="PKN90_02735"
     CDS             complement(207..944)
                     /gene="pssA"
                     /locus_tag="PKN90_02735"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNZ85228.1"
                     /translation="MSFTIKKHIPNTITCFNLFSGAIAVAFAFEGNLLFATLFIFLSA
                     VFDFLDGFLARLLGAYSELGKQLDSLADLVSFGLAPSVIVFSLFKEFPYPEAMFDYAF
                     LFPYTGFFIVVFSALRLAKFNIDESQTTSFRGLPTPANAILIGGIALSQFSFWTIPVV
                     GLYLLLALVCVSCFLLVSPLPMFSLKMKGFSIANNKVQYTFLLLSLVLLVAFFFFGFA
                     LSICLYILMSVVLAIKNRLSNKKDLHI"
     gene            complement(957..2387)
                     /locus_tag="PKN90_02740"
     CDS             complement(957..2387)
                     /locus_tag="PKN90_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNZ85229.1"
                     /translation="MKKNMLVFMLLLLLYVPVYATESTCVSGTVSGYPGTTIELYYFT
                     DEISYKQEVLTKTSIDSAGVFEFVFTLDKVRKCFVDLGAFRGILFIEPSKTYHLQPMP
                     YQPKTDAQLLNMYFTPQEVLVGLQNQPPDDINQRILQFELAIDEVWNNAMYTDLSKRY
                     LSAVIDSLEMVFPSKNDFFSIYKQSTYALLANLQVELAPNWSIQDYFISMPVHYHNPA
                     YCEAFTVLFEGYFNLLESQPNAKAFFQAYHNADIVSLREQFALLTHWNNPQLEELVIL
                     YNLWGNFSLKPKDRTTIINLLEQINHTSLYPENKKLAQEVLYRLKKAHVGSKPNVNEL
                     RTIEGKTITFSDFAGKYVYIVFASSLINETYADIIYLDKLRKKYAENLEVVFIFAQET
                     EAKTIAYTKEIGADLIFCNWNSDAQLIDAFGVQNIPSYYLLDREGFFSHSPALSPSQG
                     FERKLDAMLEVEKAAEGSSTMNKPFF"
     gene            2458..3510
                     /gene="mnmA"
                     /locus_tag="PKN90_02745"
     CDS             2458..3510
                     /gene="mnmA"
                     /locus_tag="PKN90_02745"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNZ85230.1"
                     /translation="MYTKENVLLGMSGGTDSSVSAFLLQQAGYNVIGVTFRFYEHNND
                     QTYLQKASNMAKHLHIPHYIVDKRNDFQSIVIENFTNEYLAGRTPFPCAHCNTYLKWD
                     ALLEQAHRLDCPHIATGHYVNTIKKDNCTYLSMGKDIDKDQTFFLWGMPQEAIERAIF
                     PLGNLLYHEVQNIATSNQLPVKQNKNSVGACFCPTDYRPLLKKIATEKGIQIHQGIFT
                     DEIGNFLGKHEGYPFYTIGQRRGLGLQHISPLYVKKINRETNSIIVAPKESCYQTTVH
                     VTGLICRQLVEKEKKLTAKVQYKKQACTATLDMINTSEALVTLHEPTLGMAPGQSIVL
                     YDRDILIAGGIIQNAW"
BASE COUNT         1288 a          680 c          596 g         1064 t
ORIGIN      
        1 cttcttgaaa aatagtgtac tccatcaagt tccaattcct atcaaacaac gaataggttc
       61 ctagcatctt ctccaacaca tttgtttgtg taatactgtt tgtttcgtgg gttggtggtg
      121 tgtctattgt tttttctttg gtacaagaaa aaaacacgag aagaaacaca aaaaatagta
      181 tatatggttt tatatgttgc atcaatctat atatgtaagt ctttcttatt cgatagacga
      241 ttttttattg caagtacaac tgacataagg atatacaaac agatagacaa cgcaaagcca
      301 aagaaaaaga atgcaacaag tagcacaagt gaaagcaata aaaaagtata ttgcacctta
      361 ttgttagcaa tactaaatcc tttcattttg agagaaaaca taggtaaagg cgatactagt
      421 aaaaaacaag atacacaaac caacgctagt aatagatata aacccacaac aggtattgtc
      481 caaaatgaaa attggcttaa cgcaattcca ccaattaata tcgcattcgc tggcgtaggc
      541 aagcctctaa aactggttgt ttgagactca tcaatattaa acttagccaa tcgtagtgca
      601 gaaaatacaa caataaaaaa acctgtataa gggaataaaa aagcataatc aaacattgcc
      661 tctggatacg gaaattcttt aaacaaagaa aacacaataa cactaggtgc taatccaaag
      721 ctcaccaaat cggctaacga atcgagttgt ttgcctaatt ctgaatatgc tccgagcaaa
      781 cgagccagaa aaccatccag aaaatcgaat acagctgaaa ggaaaataaa taatgtagca
      841 aacaataaat taccctcaaa agcaaaggct actgcaatgg caccagagaa taaattgaaa
      901 cacgttatcg tatttgggat atgtttttta atagtaaatg acatatggta taatgattaa
      961 aaaaatggtt tattcatagt agacgaaccc tcagcagctt tttctacttc caacatagca
     1021 tcgagtttac gctcaaaacc ttgcgatgga cttaatgctg gactatggct aaaaaaacct
     1081 tctctatcca acaaataata ggaaggaata ttttgcacac caaatgcatc tatcaattgg
     1141 gcatcactat tccaattgca aaaaattaag tccgcaccta tttcctttgt ataggctatt
     1201 gttttcgcct cagtttcttg tgcaaagata aatacaactt ctaggttttc agcatatttt
     1261 ttacgcaatt tatccaaata aataatatct gcataggttt cgtttatcaa cgaactagca
     1321 aaaactatat acacatactt ccctgcaaaa tccgaaaacg taattgtttt tccttcaata
     1381 gtacgtaatt cattcacatt gggcttactt ccaacatggg cttttttcaa cctatacaac
     1441 acttcctgtg ccaatttttt attttctgga tacaagcttg tatggttgat ttgttccaat
     1501 aaattgataa tagttgtcct atctttcggt tttagagaaa aatttcccca taagttatac
     1561 aaaattacga gctcctctaa ttggggatta ttccaatgcg taagcaatgc aaattgttcc
     1621 ctaagcgata caatatctgc attatgatac gcttgaaaaa atgctttagc gtttggttga
     1681 gattcaagga ggttaaaata tccctcaaat aaaactgtaa atgcttcaca atatgctgga
     1741 ttatgatagt gtactggcat ggatataaaa taatcttgaa tgctccaatt cggagctaat
     1801 tctacttgta aatttgctaa taatgcatac gtactttgtt tgtatataga aaaaaaatca
     1861 ttcttactag gaaagaccat ttcaagacta tctattacag cagaaagata tcttttagaa
     1921 aggtcggtat acatggcatt attccatacc tcatcaatag ctagttcaaa ttgaagaatt
     1981 cgttgattaa tatcatctgg aggttgattt tgtaacccaa caagtacctc ttgtggtgta
     2041 aaatacatgt ttaacaactg agcgtccgtt ttgggctgat agggcatcgg ttgcaaatgg
     2101 tacgtttttg atggttcgat aaacaaaata cccctaaaag cacccaaatc gacaaaacac
     2161 ttacgaactt tatcaagggt aaaaacaaac tcaaatacac ctgcagaatc aatgcttgtt
     2221 ttcgttaaaa cttcttgttt atagctgatt tcatcggtaa aatagtacaa ctctatcgta
     2281 gtacctggat atccagatac cgtgccagac acacacgtag attcagttgc atatacaggc
     2341 acatatagca acaaaaggag cataaaaacc agcatatttt ttttcattcc gtaaaaatac
     2401 aaaaagatat agacaaaaaa ataatttaac gtacttttgt acaaagttaa catgtttatg
     2461 tatacaaaag aaaatgtttt actaggaatg agcggaggaa cggatagttc tgtatcagcc
     2521 tttttactcc aacaagcagg gtataatgtt attggcgtta ctttccgttt ttacgagcat
     2581 aacaatgacc aaacctacct acagaaggct agtaatatgg caaaacacct acacatacca
     2641 cactatattg ttgataaacg caatgacttc caatcaattg taatagaaaa tttcacgaat
     2701 gaataccttg cagggcgtac cccttttcct tgtgcccatt gtaatacata tctgaaatgg
     2761 gatgccttac tcgaacaagc tcatcggtta gattgccccc atattgctac tggacattat
     2821 gtgaatacaa tcaaaaagga taattgcacc tacctctcta tggggaaaga tattgataaa
     2881 gaccaaactt tttttctttg gggcatgcct caagaagcca tcgaacgagc tattttccct
     2941 ttgggtaatt tgctctatca cgaagtgcaa aacattgcta cctccaacca acttccggtg
     3001 aaacaaaata aaaacagtgt aggtgcatgt ttttgtccta cggactaccg acctctttta
     3061 aaaaaaattg caacagaaaa aggaatacag attcatcaag gaatatttac cgatgaaatc
     3121 ggtaattttc taggaaaaca cgagggttac ccattttaca ccattggtca acgtaggggt
     3181 ttgggtttgc aacatatctc ccctttgtat gtaaaaaaaa tcaatagaga aaccaattcc
     3241 attattgtag caccaaaaga atcgtgttac caaacgaccg tacatgtaac aggattaatt
     3301 tgtcgccaac ttgtagaaaa agaaaaaaaa ctaacggcaa aagtgcagta taaaaaacaa
     3361 gcatgcactg ctacattaga catgataaat acctcggaag ccctagttac cttacacgaa
     3421 ccgacactcg gaatggctcc aggtcaatcg attgttttat acgatagaga tatacttatt
     3481 gctggtggga ttattcaaaa cgcatggtaa acatattttt tttataaaaa tatttggtat
     3541 ttatcaaaaa ggtattacct ttgcacccgc aactggtacc atagctcagt tggtagagca
     3601 aaggactgaa aatccttgtg tccccggt
//
LOCUS       DAOPUR010000110         3615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_26142_length_3615_cov_3.748596, whole genome shotgun sequence.
ACCESSION   DAOPUR010000110 DAOPUR010000000
VERSION     DAOPUR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26142_length_3615_cov_3.748596"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /gene="cmk"
                     /locus_tag="PKN90_02750"
     CDS             complement(<1..630)
                     /gene="cmk"
                     /locus_tag="PKN90_02750"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNZ85231.1"
                     /translation="MTRKKIIIAIDGYSSCGKSTMAKELATSIDYIYVDTGAMYRAVT
                     WYCIENNIVQNDVLAVDKLVSLMPNICVEFYRTGDGSVHTKLNGKDVEKEIRGMLVSQ
                     YVSKVSALRFVREAMVTLQQKMGEQKGIVMDGRDIGTVVFPQAELKIFVTADARIRAK
                     RRYDELIAKGEVVCFDDILANIRERDFLDETREESPLRKAPDALVLDNGV"
     gene            complement(645..1625)
                     /gene="porQ"
                     /locus_tag="PKN90_02755"
     CDS             complement(645..1625)
                     /gene="porQ"
                     /locus_tag="PKN90_02755"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNZ85232.1"
                     /translation="MSVVLLFSNYIQAQSLFTMSFLEQPVSAHHAALGGNNTSYLGHD
                     ISFLVSNPALLSIQSSNELSVNVASYFAGSGYGSGIYSRLLHERGMLGAGFQYMGYGE
                     FTGFDEYGVETGTFSARDVAFFVSYSQQLNNYFSIGATVKPVLGVYERYVSYALAGDM
                     GIVFMDKENGLQVGFSAKNIGVRFAGYESDESASMLPLNLSLGASKKLLHAPFVFHLT
                     MQQLQRWTYDVSTNVDSTSISFVGELARHFIVGVDVLPPSDKFWLALSYNFLRGQELP
                     VPEIFSLSGFSGGIGFFIKDVELGIGFGSYHQAATTLHISLRTDLTKFGL"
     assembly_gap    1805..1814
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1874..2560
                     /locus_tag="PKN90_02760"
     CDS             1874..2560
                     /locus_tag="PKN90_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNZ85233.1"
                     /translation="MEIKKTPKANLENKKTMAILIGLVLALGIMFIAFEWSKNEIKVY
                     DDAIQGEFVEDEEMIEVTFRNETPPPPPPPIQETVLSDVIDIRDDKEDIQTKNFDSED
                     DKNKKVVIQAPIAAPVEEPEENRIHVVVERMPEFPGGEAAMNQFINRTIRYPVIAQEN
                     GIQGRVVVQFVVNTDGKIVDVEVVRGVEESLDKEAIRVVKAMPPWNPGRQGGKNVRVK
                     YTLPIRFRIG"
     gene            2618..>3615
                     /gene="hflX"
                     /locus_tag="PKN90_02765"
     CDS             2618..>3615
                     /gene="hflX"
                     /locus_tag="PKN90_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNZ85234.1"
                     /translation="MISHSEIHIERVVLVGLITQQQNETKSKEYLKELAFLAETAGAI
                     VIKEFYQRVELPNTKTYVGKGKLLEIKNFIQEQEINLVIFDDELSPSQLRNIENTLEA
                     KALDRTSLILDIFAKRAQTAHAKTQVELAQYEYFLPRLTRLWTHLERQKGGIGLRGPG
                     ETQIETDRRIILDKIARLKAELIHIDKQKTIQRKNRGKMVRVALVGYTNVGKSTLMNL
                     LSKSNVFAENKLFATLDTTVRKITINNLAFLLSDTVGFIRKLPHGLVESFKSTLDEVR
                     EVDILVHIVDISHPAFEEQIKVVEKTLHEINNKEKLTILVFNKIDAFTYEKKDEDD"
BASE COUNT         1332 a          757 c          568 g          948 t
ORIGIN      
        1 aaccccatta tctaagacca atgcatcagg ggctttacgc aagggactct cttctcgagt
       61 ttcatctaaa aaatcccgct ctcgtatatt tgctaaaata tcatcaaaac aaacaacctc
      121 tcccttcgct atcaattcat catatcgtct tttagcacga atacgtgcat ctgctgttac
      181 aaatattttt aattcagcct gcggaaaaac aacagttcca atatcacgtc cgtccatcac
      241 aatccccttt tgttctccca tcttttgctg taaagttacc atagcttctc gcacaaatcg
      301 taaggcactt accttgctta catactgaga cacaagcatc cctctaattt ctttctctac
      361 atccttccca tttagctttg tatgaacaga gccatcaccc gttctataaa actcaacaca
      421 tatgtttggc ataagagaaa cgagcttatc taccgcaagc acatcatttt gtactatatt
      481 attctcaata caataccatg ttacagccct atacatagct ccagtatcta catatatata
      541 atctatagag gtagctaatt cctttgccat cgtactttta ccacatgatg aatatccatc
      601 tatggctatg ataatctttt tcctagtcat acaaaacgca ttttctacaa tccaaatttc
      661 gttaaatctg tacgcaatga gatatgtaaa gtagttgcag cctgatggta tgacccaaaa
      721 ccaatgccta actcaacatc ctttataaaa aaaccaatac cgccagaaaa accactcaaa
      781 gaaaatattt caggaacagg aagttcctgt ccgcgtaaaa aattatacga taatgctagc
      841 caaaacttat cggaaggagg caatacatct acccccacaa taaaatggcg agccaactcc
      901 cccacaaaag atatggatgt agaatcaaca tttgtagaca catcatacgt ccaacgttgt
      961 aactgttgca ttgttagatg aaacacaaaa ggagcatgca atagcttttt tgaggctcca
     1021 agactcaaat ttaagggcaa catagaagct gattcatcag attcataccc agcaaatctt
     1081 actccaatat ttttggcaga gaacccaacc tgcaagccat tttccttgtc cataaaaact
     1141 atccccatat ccccagccaa tgcatatgac acatagcgtt cgtaaacccc caacacaggc
     1201 ttaaccgtag ccccaatact aaaataatta ttcaactgtt gtgaataact cacaaagaac
     1261 gcaacatctc gtgccgaaaa cgtccccgtt tctacaccat attcatcaaa tccagtaaac
     1321 tctccatacc ccatatactg aaatcccgcc cctaacatgc ctcgctcatg taacaaacga
     1381 ctatatattc ctgaaccata acctgaccca gcaaaataac ttgccacatt tacagacaac
     1441 tcgttggatg attgaataga caataacgcc ggattggata ctaaaaaaga aatatcatgc
     1501 cccaaataag atgtattatt ccctcctaaa gcagcatgat gagcagaaac cggttgctct
     1561 aaaaaactca tagtaaaaag cgattgtgcc tgtatgtagt tactaaacag taaaacaacc
     1621 gacataaata tattaattct taccttcatt tcgttatact aaaaaaaaca ttcaaatata
     1681 catcttttgt ggtaaataac gatgttacta agcaacaaaa tcaagttcaa aaaaaatatc
     1741 agtaaaaaat tacaggatat acaagctatt aatggtcaag tggagcaagt ggaggttctg
     1801 tttcnnnnnn nnnnattaaa aaaaaatatt gtacatttgc aagcaactgt gtaaacgaca
     1861 aacttcaacg tatatggaaa taaaaaagac cccaaaagcc aatttggaaa acaaaaaaac
     1921 aatggcaatt ctgattggtt tagttctagc ccttggaatt atgttcattg cattcgaatg
     1981 gtctaaaaat gaaatcaaag tgtatgatga tgcaattcaa ggagaatttg tagaagacga
     2041 agagatgatt gaggtaactt tccgtaatga aaccccgcca ccaccaccgc cacctatcca
     2101 agaaaccgtt ttaagtgatg tcattgatat tcgtgatgac aaagaggata ttcagacaaa
     2161 aaattttgat tctgaagatg ataaaaacaa aaaagtagta attcaagctc ctattgcagc
     2221 accagtagaa gaacctgaag aaaatagaat acacgtagta gtagagcgta tgccagagtt
     2281 ccctggtgga gaggctgcta tgaatcaatt catcaaccga accattcgct accctgttat
     2341 tgctcaagaa aacggaatac aagggcgtgt agtagtgcaa tttgtggtaa atacagacgg
     2401 taaaattgtg gacgtagaag ttgtacgcgg agtagaagaa agtttagaca aggaagctat
     2461 tcgtgtagta aaagcaatgc caccatggaa tccaggacga caaggaggta aaaatgtgcg
     2521 tgtaaaatac acattaccaa ttcggttccg aataggatag atattacaaa aacaaagcct
     2581 taccgatttg gtgaggcttt tttaacctat ataagctatg atctctcatt ctgaaataca
     2641 tattgaacgc gtagtattag ttggtctaat aacccaacaa caaaatgaaa caaaatcaaa
     2701 agagtatctc aaagaactcg cgtttttggc agaaactgct ggagctattg tcataaaaga
     2761 gttttaccaa cgagtagagc tcccaaatac aaaaacctat gtgggaaaag gaaaactact
     2821 cgaaataaaa aactttatac aagaacaaga aatcaacctc gttatttttg atgatgaatt
     2881 atccccttct caactccgca atatcgaaaa cacactagaa gctaaagccc tagacagaac
     2941 aagtctgatt ttagatattt ttgcaaaaag agcacaaaca gctcacgcaa aaacacaagt
     3001 tgaactcgct caatacgaat atttcttacc ccgattaaca cgtttatgga ctcacctcga
     3061 acgccaaaaa ggaggtatcg gattaagagg tccgggtgaa actcaaattg aaacagacag
     3121 acgtataata ttagacaaaa tagctagact taaagcagaa ttaatccata tagataaaca
     3181 aaaaaccatt caacgcaaaa atcgcggcaa aatggttaga gtagcattag ttggttacac
     3241 aaatgtaggc aaatcaactt taatgaactt gctcagtaaa tcaaatgtat ttgccgaaaa
     3301 taaattattc gccactctcg acactaccgt tagaaaaatt acgattaaca atctcgcatt
     3361 tctactatct gacacagtag gttttatcag aaaattgcct catggcttag tcgaatcatt
     3421 caagtcaact ctagatgaag taagagaagt cgatatacta gtacacatcg tagatatttc
     3481 tcatcctgca tttgaagaac aaattaaagt agtagaaaaa acactgcatg aaattaataa
     3541 caaagaaaaa ttaaccattc ttgtctttaa caaaattgac gcgtttacgt acgaaaaaaa
     3601 agatgaagat gattt
//
LOCUS       DAOPUR010000111         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_26497_length_3580_cov_2.916879, whole genome shotgun sequence.
ACCESSION   DAOPUR010000111 DAOPUR010000000
VERSION     DAOPUR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26497_length_3580_cov_2.916879"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1911
                     /gene="glmS"
                     /locus_tag="PKN90_02770"
     CDS             67..1911
                     /gene="glmS"
                     /locus_tag="PKN90_02770"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNZ85235.1"
                     /translation="MCGIVGYIGKQDAYPILIKGLHRLEYRGYDSAGVALINEAGSLN
                     VYKSKGKVSDLEQSATSKDISGTIGVAHTRWATHGEPNNENAHPHYSQSESLALIHNG
                     IIENYMVIKKELIRNGYTFKSSTDTEVLVQFIEYVKKTNQVDLCHAVQIALREVVGAY
                     AIAVLDKNDPKQIIAARKSSPLVVGIGDGEFFLASDATPIIEYTNKVVYVNDEEIAVI
                     HIDKGLKLLNLVDIEQTAEVKQLEMNLSQLEKGGYEHFMIKEINEQPHTLIDCIRGRI
                     NVEGTDVKLSGVIDHQDKFLKAKRIIIVACGTSWHAGLIGEYLIEDLCRIPVEVEYAS
                     EFRYRNPIIQPDDIVIAISQSGETADTLAAVEIAKSQGAFIYGVCNVVGSSIPRATHS
                     GSYIHVGPEIGVASTKAFTGQITVLSMLALTVAKLRKTIQPEEYRKLIRELQAIPNKM
                     QEVLKLDNQIKDLAKMFTYAHNFIYLGRGYNYPAALEGALKLKEISYIHAEGYPAAEM
                     KHGPIALIDAEMPVVAIATKDSIYDKTVSNIQEIKARKGRVIAVVTEGDDVVSELADY
                     CIAIPESLEYFAPIVSAIPLQLFAYYIAVNKGRNVDQPRNLAKSVTVE"
     gene            complement(2004..2546)
                     /locus_tag="PKN90_02775"
     CDS             complement(2004..2546)
                     /locus_tag="PKN90_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNZ85236.1"
                     /translation="MTYVFLANGFEEIEAISTIDILRRAGIETTTVSIEPQLAVIGAH
                     NIVVVADQLFAEGAYNNAECLLLPGGMPGTSNLNKHEKLKELISQHVHSGKLMGAICA
                     APLILGELNLLQGKIATCYPSFETYLHGATISDDKVADAGEIVTGKGPGFSMDFALTI
                     VKKLKGACAADEVAKGLLLQ"
     gene            2663..2736
                     /locus_tag="PKN90_02780"
     tRNA            2663..2736
                     /locus_tag="PKN90_02780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2697..2699,aa:Ala,seq:ggc)
     gene            complement(2886..3173)
                     /locus_tag="PKN90_02785"
     CDS             complement(2886..3173)
                     /locus_tag="PKN90_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNZ85237.1"
                     /translation="MENIIDKIRTELRHSIDEKTLATSQKFFKEKIKLYGVKVPTVNK
                     ISREYFKLIDCKTKSEIVDYVIKNKSIMPRTALRYAIEKMPKELKIKAMER"
BASE COUNT         1151 a          558 c          691 g         1180 t
ORIGIN      
        1 aaggagaaaa taaatatctt tgtagcaaaa tatgttatag aatacacttg ttaaaataac
       61 gaatttatgt gtggaattgt aggatatata gggaagcaag atgcttatcc aatactaatt
      121 aaaggcttac atcgtttgga ataccgtggt tatgatagtg ctggtgtggc tttaatcaac
      181 gaagctggta gcttaaatgt gtataaatcg aaaggaaagg tatctgattt agaacaatca
      241 gctacttcaa aagatattag tggtactata ggagtggcac atactcgatg ggctacacat
      301 ggtgagccta ataatgaaaa tgcccatcct cattattctc aatcagaatc gctggcatta
      361 attcataatg ggattatcga aaattatatg gtaatcaaaa aagagctaat tcggaatggt
      421 tatacattta agagctctac agatactgag gtattggtac aatttataga atatgtaaaa
      481 aaaacgaatc aagtagactt gtgtcatgct gtacaaattg cgttgagaga agtagtaggt
      541 gcatatgcta ttgctgtgct tgataagaat gatccaaagc aaataattgc tgctcgcaaa
      601 agtagtccat tagttgtagg gattggggat ggcgagtttt ttttagcctc tgatgctact
      661 ccaattattg aatatacgaa taaagtagtg tatgtgaatg acgaagaaat agccgtgata
      721 catatagata agggtttaaa gttgctcaat ttagttgata ttgaacaaac agctgaagta
      781 aaacagttgg aaatgaatct tagtcaactt gaaaagggtg ggtatgagca ttttatgatt
      841 aaggaaatca acgaacaacc acacacctta atagattgta tcagaggacg aattaatgtt
      901 gagggtactg atgtaaaatt gtctggtgtg attgatcatc aagataaatt tttgaaagct
      961 aaacgtatta ttattgtagc atgtggtact tcttggcatg caggattaat aggcgaatat
     1021 ttgatagaag atttgtgtcg tatacccgta gaggttgaat atgcatcgga atttaggtat
     1081 cgcaatccaa ttattcagcc agatgatatt gtaatagcca tctctcaatc aggagaaact
     1141 gccgacacct tagctgctgt agaaattgct aaaagtcaag gagcatttat ttatggtgtc
     1201 tgcaatgtgg taggatcgtc tatacctcgt gcaacacatt cgggatcgta tatacatgta
     1261 ggtccagaaa ttggtgttgc atctactaag gcatttactg gacaaataac cgtattgtca
     1321 atgttggcgt tgactgtagc caaattgcgt aaaactattc aaccagaaga gtacagaaaa
     1381 cttattcgag aattgcaagc tattccaaat aaaatgcaag aagtactcaa actagataac
     1441 caaataaaag atttggcaaa aatgtttacg tatgcccata attttatata tttgggtcga
     1501 ggctataatt atccagcagc ccttgaggga gcattaaaac taaaagaaat atcatatatc
     1561 catgccgaag gttatcctgc tgcagaaatg aaacatggtc ctatagcgtt gattgatgca
     1621 gaaatgccag tagtggcaat agctactaaa gatagtattt atgataaaac tgttagcaat
     1681 attcaagaaa taaaagcccg taaaggaagg gttattgccg tggttacaga aggagacgat
     1741 gtggttagcg agcttgctga ttactgtatt gctataccag aatcattaga atattttgct
     1801 cctattgttt cggctattcc attgcagtta tttgcttatt atatagctgt aaataagggt
     1861 cgcaatgtag accaaccacg taatttagct aaatcggtaa cggtagagta aaaactattt
     1921 attttgttta tattaaaaag aatcgctcca aaatagggcg attcttttgt tagatagagg
     1981 ttaaaaatca atgtgtaacg aaactattgc aacaacaatc ccttagctac ttcgtcagca
     2041 gcacaagccc cttttagttt ttttactatt gttagagcaa aatccatact aaatcctgga
     2101 ccttttcctg taactatttc gccagcatct gctactttat catcacttat agtagctccg
     2161 tgtaaatatg tttcaaaact tgggtaacaa gtggcaattt ttccttgtaa taaattcaac
     2221 tcgcccaaaa taagtggtgc agcacaaatg gctcccatta atttaccact atgtacatgc
     2281 tggctgatta attcttttag tttttcatgt ttatttaagt tcgaagtgcc aggcatacct
     2341 cctggtaaca gtagacactc agcattatta tatgctcctt ctgcgaataa ttggtcagca
     2401 acaaccacaa tattgtgagc tccaataaca gctagttgag gttcgattga aacagttgta
     2461 gtttcgatac ctgctctgcg taagatgtcg atggtagaaa ttgcttctat ttcttcaaaa
     2521 ccattggcta aaaaaacgta cgtcatagta ttttgtattt aaatgtatga ataattgtgt
     2581 gaaaaatact gtttgctatc taacaaacat ttacaaatat agtaattatt ttggaaaaga
     2641 ttgtatattt gcactgcaaa aagggggttt agctcatctg gctagagcgc gacactggca
     2701 gtgtcgaggt gaccggttcg agtccggtaa tctccacaaa acgaccaaag ttcatgattg
     2761 atgggctttg gtcgttttag taaataggca tatattcctt tgtaatttgc ttcataattc
     2821 atctgccatc acactatttt cactgataca tctgacctga cggagttctt ttttattttt
     2881 ccaaattatc tctccattgc cttgattttc agttcttttg gcattttttc aattgcatat
     2941 cgcaatgctg tacgaggcat aatggatttg ttttttatta cataatcaac tatttctgat
     3001 tttgttttac aatcaatcag tttaaaatac tctctactaa tcttgttgac agttggaacc
     3061 ttaactccat acaactttat cttttctttg aaaaattttt gactggtcgc caacgttttt
     3121 tcatcaattg aatgccttaa ttcagttcta attttgtcta ttatattttc cattttgaac
     3181 gtattgttta tccgcgaaat ggctttttta gcatgcggta taacggtagt ttatttaata
     3241 cctccgttta gtataatttt tcttcaaatg taatacgttt aaacgaactc tcaaaatata
     3301 ctggagcttt ttgcttgtaa tgggtggggg tagtgtaatc tagtcgttac aaacagacaa
     3361 accataagca cctcgttaca aacgaggggt agggaggatg atacaaagag ttgatatttt
     3421 tgtttctttt tttatttgaa aaatatatgt atgtagggaa acttgcgtat atttgtgagt
     3481 tagcggttat tttatgagcg aaacagacac attagagaaa ttaccataaa atgaaaagat
     3541 tatctgtagt actgaatttg attataatta gtagtattat
//
LOCUS       DAOPUR010000112         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_26514_length_3578_cov_3.813511, whole genome shotgun sequence.
ACCESSION   DAOPUR010000112 DAOPUR010000000
VERSION     DAOPUR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26514_length_3578_cov_3.813511"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /gene="eno"
                     /locus_tag="PKN90_02790"
     CDS             complement(<1..1237)
                     /gene="eno"
                     /locus_tag="PKN90_02790"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNZ85238.1"
                     /translation="MCRIEKIVAREVLDSRGNPTVEVDVTLESGIVGRAAVPSGASTG
                     ENEAIELRDGDKGRYLGKGVLKAVANVNNVIAPELIGMCAMEQRAIDKKMIALDGTKT
                     KSKLGANAILGVSLAVAKAAAEYLGLPLYRYIGGTNAFTLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGAKSFREGLRMGAEVFHSLKKVLHDKGLSTAVGDEGGFAPNLAGGTEE
                     ALESILTAVKNAGYVPGKDVTIGLDCAASEFYKNGIYDYSKFEGPNGKKRTSAEQADY
                     LAELIANYPIDSIEDGMGEGDWDGWKLLTDKIGSKCQLVGDDLFVTNVEYLKKGIDLG
                     CANSILIKVNQIGSLSETLDAIEMAHRAGYTSVTSHRSGETEDATIADIAVATNSGQI
                     KTGSLSRSDRMAKYNQLLRI"
     gene            complement(1391..2203)
                     /locus_tag="PKN90_02795"
     CDS             complement(1391..2203)
                     /locus_tag="PKN90_02795"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNZ85239.1"
                     /translation="MLAKINETATYLKGKMQRFPKVGIILGTGLGELASQITEKQEIE
                     YQTIPNFPVSTVEGHSGKLIIGKLGTTEVLAMQGRFHYYEGYSMQEVTFPVRVMKALG
                     IETLLVSNASGGVNPSFEIGDLMIITDHINTFPEHPLRGKNHPELGVRFPDMSQAYSK
                     ELISKAKKIAARNTIKVVEGVYLGTSGPTFETPAEYVYFRNIGADTVGMSTVPEVIVA
                     VHSGIKVFAMSIITDLGVPGKIVEVSHEEVQHIANQVQPKMTQIMKELIAEL"
     gene            complement(2142..3236)
                     /gene="lpxK"
                     /locus_tag="PKN90_02800"
     CDS             complement(2142..3236)
                     /gene="lpxK"
                     /locus_tag="PKN90_02800"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNZ85240.1"
                     /translation="MLLWPISWLYGILMSIRNMLFDKHILPTESFPISVISVGNITVG
                     GTGKTPHTEYLVSILKKKFNVAVLSRGYKRKTVGYIYANATASAQTIGDEPFQIYQKH
                     PDIVVAVDANRRNGIKQILLSHPQTDVVLLDDAFQHRYVEANQTILLVDYNRPITEDN
                     MLPYGNLRESIDGIKRADIILITKCPPNLTPIEARNIHKSLNLLAYQNIFYTSFAYGD
                     VKSVFIPNQIVTTSIIQEQQFGILLVTGIAQPEPLLDYICTLTPYVEKLLFPDHHFFT
                     QQDMGIIIEMFENMKQEKRLIMVTEKDAARLVNDPLFPDHLKPYTCMLPISVNFLFQQ
                     QKIFNSKIIDYVSKNQRNSNVPKRKNATLS"
     gene            complement(3260..>3578)
                     /locus_tag="PKN90_02805"
     CDS             complement(3260..>3578)
                     /locus_tag="PKN90_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNZ85241.1"
                     /translation="LLPLAGGRVWTGSDALTHGLVDKIGGLQQAIQSAARMASITSYE
                     VEHLPKKKTVWESMLEDTTIQIQSLLTKNIPLHEYRLIRSFIDNKTRIIIQARMEENS
                     EIW"
BASE COUNT         1007 a          686 c          647 g         1238 t
ORIGIN      
        1 cgatacgaag tagttggttg tatttagcca tacggtcaga tcggctcaac gaacctgttt
       61 tgatttgacc agaatttgta gctaccgcaa tatctgcaat agttgcatct tcagtttcgc
      121 cagagcggtg agaagtaaca gaagtataac cagcacggtg agccatttcg atagcgtcta
      181 atgtttcaga aagagatccg atttggttaa ctttaataag gatagagtta gcacaaccta
      241 aatcgatacc ttttttcaag tattctacat tagttacaaa taagtcatca cctactaatt
      301 gacatttact accaattttg tctgttaata gtttccatcc gtcccagtcg ccttcaccca
      361 taccatcttc gattgagtca ataggatagt tagctattaa ttctgctaaa tagtctgctt
      421 gttcagcaga ggtacgtttt tttccattag gaccttcaaa ttttgaatag tcatagatgc
      481 catttttgta aaattcagaa gcagcacaat ccaatccaat tgtaacatct tttcctggaa
      541 catatcccgc attttttact gcagtaagga ttgattctag agcttcttca gtacctccag
      601 caagatttgg agcaaaacct ccttcgtcgc caacagcagt acttaatcct ttatcgtgta
      661 atactttttt caaagaatgg aaaacttcag cacccatacg taagccttcg cggaaagatt
      721 tagcacctac agggcgaatc ataaattctt ggaatgcgat aggagcatca gagtgcgagc
      781 ccccgttaat gatattcatc ataggtacag gcaatgtaaa tgcgtttgta ccgccaatgt
      841 aacggtataa tggtaatcca aggtattcag cagcggcttt agccacagcc aaagaaacgc
      901 ctaagatagc attagctcct aattttgatt ttgtttttgt accatctaat gcaatcattt
      961 ttttgtcgat agcacgttgt tccatagcac acataccgat aagttcaggt gcaattacgt
     1021 tgtttacgtt agcaacagct tttaatacac ctttgcctaa gtagcgacct ttgtcgccat
     1081 cgcgtaattc aatagcttcg ttttcgccag ttgatgcacc tgatggtaca gcagctctgc
     1141 ctacaattcc tgactctaac gttacatcaa cttctacagt tggatttcca cgcgaatcaa
     1201 gtacttcgcg ggctacaatt ttttcaattc tacacatgtt cttttttatt tataaattga
     1261 tattcactaa ttgtgtttca aaatttgacg tacaaaagta caaaaaaaat cttgactatt
     1321 aaatatgtct gtattatttt ttgtttttaa tatttcccct tagcatattc attacaccaa
     1381 tgttacaaag ctataattcg gcgataagtt ctttcattat ttgggtcatt ttgggttgca
     1441 cctgattagc gatgtgttgt acttcttcgt ggcttacttc cacaatttta ccaggaaccc
     1501 ctaaatctgt aataatggac atagcaaaaa ccttaatgcc actatgaacg gcaacaatta
     1561 cttcaggaac tgttgacata ccaacggtgt ctgccccaat atttctaaaa taaacgtatt
     1621 cggcaggagt ttcaaaagta ggaccagagg tgccgagata aacaccttct actaccttaa
     1681 tcgtgtttct tgctgcaatt ttttttgctt ttgaaattaa ttcctttgaa taagcttgac
     1741 tcatatctgg aaaacgaacg cctaattcag gatggttttt acctctcagt ggatgttctg
     1801 ggaatgtatt gatatgatcc gtgataatca ttaaatcccc aatttcaaat gagggattta
     1861 ctcctccact ggcattggaa actaataatg tttcaattcc taaggctttc ataacacgta
     1921 cagggaaagt aacctcttgc atagagtatc cttcgtagta gtggaaccta ccttgcatcg
     1981 ccagaacttc agttgttcct aatttaccaa taatcaattt gccactatgc ccttctaccg
     2041 ttgaaactgg aaagtttgga attgtttggt actctatttc ttgtttttct gtaatttgag
     2101 aagctaattc tcccaatcct gtgcctaaaa taatacccac tttaggaaag cgttgcattt
     2161 ttccttttag gtacgttgct gtttcgttga tttttgctaa cataatctat aattttagaa
     2221 ttaaatattt tttgttgttg aaataaaaaa ttcacactga tgggtaacat acatgtgtac
     2281 ggttttagat ggtcaggaaa aagtgggtcg tttaccaaac gtgctgcatc tttttctgtt
     2341 accattatca ggcgtttttc ctgtttcata ttttcaaaca tctctatgat gattcccata
     2401 tcttgttggg taaaaaaatg atggtcgggg aatagtagct tctctacata gggagtcagt
     2461 gtgcaaatgt aatctagcag aggttctggt tgtgctattc ctgtaactaa aagaattcca
     2521 aattgttgtt cttgaatgat gcttgtagtt actatttggt tgggtataaa tacgctcttg
     2581 acatcaccat atgcaaagct tgtatagaaa atgttttggt acgctaataa gtttaaggat
     2641 ttatgtatat ttcttgcttc aataggtgtt agattgggtg ggcattttgt gatgagaatg
     2701 atgtctgcac gtttaatgcc atcaatagat tctcgtaaat taccatatgg caacatattg
     2761 tcttcagtta taggtctgtt ataatcaact aacaatattg tttgatttgc ctctacataa
     2821 cgatgttgaa aagcatcatc aagtagtaca acgtctgttt gtggatgaga tagtaaaatt
     2881 tgtttaattc cattacgacg gttagcatca acggctacca caatatcagg atgtttttga
     2941 tatatttgaa aaggctcgtc tcctattgtt tgggctgaag ccgttgcatt ggcatagata
     3001 tatcctaccg ttttgcgttt gtagcctcta cttagtactg ctacattgaa tttctttttt
     3061 aagatagata ctaaatattc agtatgagga gttttaccag taccacctac tgtgatgtta
     3121 ccaacagaaa ttacactgat tggaaagctt tccgttggta atatgtgttt gtcaaacagc
     3181 atgttgcgta tactcattaa gatgccgtat agccatgaga ttggccacaa tagcatgcgt
     3241 ataaaaatgt tttttcgagt taccatattt cactattttc ttccatccga gcctgtatta
     3301 ttattctagt tttattgtca atgaaggaac gtattaacct atactcatgc aggggaatgt
     3361 ttttagtaag caaagattga atctgtatcg ttgtatcttc taacatgctt tcccatactg
     3421 ttttcttttt agggaggtgt tctacctcat agcttgtaat tgatgccata cgagcagccg
     3481 attgtatggc ttgttgtagc cctcctattt tgtctactaa gccatgtgtt aaagcgtccg
     3541 atcctgtcca tactctgcct ccagctagtg gtagtaat
//
LOCUS       DAOPUR010000113         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_26528_length_3576_cov_3.579949, whole genome shotgun sequence.
ACCESSION   DAOPUR010000113 DAOPUR010000000
VERSION     DAOPUR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26528_length_3576_cov_3.579949"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKN90_02810"
     CDS             <1..200
                     /locus_tag="PKN90_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNZ85242.1"
                     /translation="GNPVIDDHYYINKIANIPCIDIIHYSNQTGFVDTWHTIYDTMEN
                     IDKTTLGVVGRVVTAVVYSEK"
     gene            206..>532
                     /locus_tag="PKN90_02815"
     CDS             206..>532
                     /locus_tag="PKN90_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNZ85243.1"
                     /translation="MLLPYVHKDLIECGCDEAGRGCLAGAVYAAAVILPTDFCCPELN
                     DSKKLTEKQRYALRPYIEQHALAFAIGVVSESEIDEINILNASFLAMHRAIEGLPIQP
                     QHLLIDG"
     assembly_gap    533..542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <543..786
                     /locus_tag="PKN90_02820"
     CDS             <543..786
                     /locus_tag="PKN90_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNZ85244.1"
                     /translation="FKNKTTIPHTCIVKGDGKYASIAAASILAKTYRDDYMSTLHISY
                     PCYGWLQNKAYPTKAHRNAIRENGVSPFHRKTFRLL"
     gene            complement(792..2042)
                     /locus_tag="PKN90_02825"
     CDS             complement(792..2042)
                     /locus_tag="PKN90_02825"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNZ85245.1"
                     /translation="MAQHMEWGAKALQAARRLNLLTDATINQILVKVAEEAIANTSYI
                     LAENQKDLDAMDKNNPMYDRLLLSTDRIQGIAADIRNVASLPSPLGRILEERTLPNGM
                     FLTKKSVPFGVIGIIYEARPNVSFDVFSLCLKSGNVCVLKGGTDAYHSNLAIVSVIKN
                     VLKQFDIDENCIHLLPAGREATADLLNAVDYVDLIIPRGSQGLIDFVRNNAKVPVIET
                     GAGVCHTYFDEFGDFKKGQSIIYNAKTRRVSVCNALDCLIINEKRLADLASLCLPLAE
                     KEVVIEADIKAYTFLHGKYPTHLLTKATAESFGTEFQSYRMSIKTVSNMEQAIEHIAT
                     YSSKHSEAIISENEHAIHQFEQQVDAACVYSNASTAFTDGAQFGMGAEIGISTQKLHA
                     RGPMALPELTSYKWIVKGNGQTRS"
     gene            complement(2044..3126)
                     /gene="proB"
                     /locus_tag="PKN90_02830"
     CDS             complement(2044..3126)
                     /gene="proB"
                     /locus_tag="PKN90_02830"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNZ85246.1"
                     /translation="MVLKFKKIAVKIGSNVLTQKDGTLDIPRIEKLVTQIAQLHKAGL
                     EIILISSGAVAAGKSQLKVSKKIDPISARQLFSAVGQVTLMNHYHRLFSEHGIFCGQV
                     LTTKENFGSRRHYLNQKNCMSEMLAHNVIPIVNENDTISVTELMFTDNDELSGLIATM
                     MGMEALIILSNIDGIYNGNPEDKNSQVIETVEANKNLSQYIQTGKSSFGRGGMLTKTT
                     IARKVADEGIHVFIANGKKDNILLDLVNKEKHVICTQFIANRKEVSSVKKWIAHSDGF
                     AKGEVHVNAGARKALTGNKATSVLFVGITEILGEFEKDDIVKIIDENGKQLGVGKTQY
                     DNKKATQLIGKKGQRPLVHYDYLYLD"
     gene            3207..>3576
                     /locus_tag="PKN90_02835"
     CDS             3207..>3576
                     /locus_tag="PKN90_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HNZ85247.1"
                     /translation="MKKYLLLGTVLLVLVGCSTVALTGRKQLMLVSDAEILEASNLHY
                     TEFMSTAKVSTNAAATKQIKQIGANIAKAVETYLKENGREADIASFAWDFTLVQDTTI
                     NAFCLPGGKVVFYEGILPICA"
BASE COUNT         1026 a          683 c          656 g         1201 t
ORIGIN      
        1 caggtaatcc tgttattgac gatcattatt atattaacaa aatagccaat attccctgta
       61 ttgacattat tcattatagt aatcaaacgg gttttgttga tacttggcat actatctacg
      121 acaccatgga gaatattgat aaaacgacct taggtgtagt tggtagagtt gtaacggctg
      181 ttgtttattc agaaaaataa ggtgtatgtt gttgccatat gtacataaag acttgataga
      241 atgtggttgc gatgaagctg gaagaggttg tttggctggt gcagtatacg cagctgcagt
      301 aattttgcca accgattttt gttgcccaga actaaacgac tcgaaaaaat taaccgaaaa
      361 acaacgctac gccttacgtc catatattga gcaacatgct ttggcgtttg ccattggtgt
      421 ggtttctgag agtgaaattg atgaaataaa tatcctcaat gcctcttttt tagccatgca
      481 tcgtgctatt gagggtttgc ctattcaacc tcaacattta ttaatagatg gcnnnnnnnn
      541 nnatttaaaa ataagaccac aattccgcat acatgcatag taaaaggaga tggtaaatat
      601 gcatccattg ccgctgcttc tattcttgcc aaaacgtata gagacgacta tatgtccaca
      661 ttgcatattt cttatccctg ctatggttgg ctacagaata aagcttatcc taccaaggca
      721 catcgcaatg ccatacgaga aaacggtgtt tctccctttc accgaaaaac gtttcggtta
      781 ctgtaaagcg tttacgagcg agtttgaccg tttcctttca caatccattt gtagctagta
      841 agttctggta aagccatagg acctcgggcg tgaagttttt gtgtgctaat gccaatttct
      901 gcccccatcc caaattgtgc tccatcagta aatgcggtag aagcattgct atatacacaa
      961 gcagcatcta cttgttgttc aaattgatgt atggcatgtt cgttttcaga aataatcgct
     1021 tcgctatgct ttgagctata ggtggcaatg tgttcaatag cttgctccat attgctcact
     1081 gtttttatac tcatacgata gctctgaaat tcggtgccaa aactttctgc tgtggcttta
     1141 gttagtaaat gcgtagggta tttaccatgt aagaaagtat aagcttttat atcagcctct
     1201 atcactactt ctttttctgc taatggaagg caaaggcttg ccaagtctgc taatcttttt
     1261 tcattgatta tcaaacaatc tagggcattg catacactaa cgcgacgagt ttttgcatta
     1321 taaataattg attgcccttt tttaaagtcg ccaaattcat caaaatacgt atggcaaacc
     1381 ccagctcctg tttctattac aggtactttg gcattgtttc gcacaaaatc gataagcccc
     1441 tgactgccac gagggataat taagtctaca taatcaacag cattgagtaa gtctgccgta
     1501 gcttccctac cagctggcaa tagatggata caattttcat ctatgtcaaa ctgttttaat
     1561 acatttttta ttaccgatac gattgccaaa ttagaatgat aagcatctgt accaccttta
     1621 agcacacata cgtttcctga ttttaaacac aaggagaaaa catcgaaact aacgttagga
     1681 cgtgcctcat aaattatacc aatcactccg aaaggaacac tttttttagt caaaaacata
     1741 ccatttggca aggttctttc ttccagtatg cgaccaagag gcgagggcaa tgatgccaca
     1801 ttgcgtatat cagcagctat accttgtatc ctatctgtac tcaatagtaa acggtcgtac
     1861 attggattat ttttatccat tgcatccaaa tctttttggt tttcagccaa aatataggaa
     1921 gtattggcaa tggcttcttc agccactttt actagtatct ggtttatagt tgcatctgta
     1981 agcaagttta atctacgtgc tgcttggagt gctttagctc cccattccat gtgttgtgcc
     2041 atattagtct aagtataaat aatcataatg aaccaaagga cgttgtcctt tttttcctat
     2101 taattgtgtt gcttttttat tatcgtattg tgtttttcct acgcctagtt gtttgccatt
     2161 ttcgtcaatg atttttacaa tatcatcttt ttcaaattcg cctagtatct cggtaatgcc
     2221 aacaaacaat accgaggtag ccttgttgcc tgttagtgct tttcttgctc ctgcatttac
     2281 atgtacttct cctttagcaa atccgtcgct atgagcaatc cattttttta cacttgatac
     2341 ttctttgcga ttggcaataa attgggtgca aattacgtgt ttttctttat ttaccaaatc
     2401 gagcagtata ttgtcttttt ttccattggc aataaatacg tggattcctt cgtcagctac
     2461 ttttcgtgca atagtagttt tggtaagcat accgcctcgt ccaaacgatg atttacctgt
     2521 ttgaatatat tgcgaaaggt ttttgtttgc ttctaccgtt tctattacct gagagttttt
     2581 gtcctcggga tttccattat aaataccatc tatattgctc aaaattatca atgcctccat
     2641 ccccatcatt gtggcaatta gaccagagag ttcgtcgttg tcggtaaaca tgagttcggt
     2701 aaccgaaata gtatcattct cgttcacaat aggaattaca ttgtgtgcaa gcatttcgct
     2761 catgcagttt ttttgattga gatagtgcct gcgagatcca aaattttctt ttgtagtaag
     2821 tacttgtcca caaaagatac catgttcgga aaatagacgg tggtaatgat tcattagagt
     2881 tacctgaccg acagctgaaa ataattgcct agccgaaatg gggtctatct tttttgaaac
     2941 ttttagctga cttttaccag cagccacagc accagaagag atgagtataa tttctaatcc
     3001 tgctttatgc aattgggcta tttgcgttac cagtttttca atacgaggaa tatcaagtgt
     3061 cccatctttt tgagttagga cgttacttcc aatctttacc gctatttttt taaattttaa
     3121 gaccatttag ggtagtttat gtaataaaat ttttactttt gcaaaagtaa aaataaattg
     3181 ttaattagac gaataaaaaa atgaatatga aaaagtattt actccttgga acagtcttac
     3241 ttgtattagt tgggtgctca acagtagcgt taacaggcag aaaacagctc atgttggtat
     3301 cggatgcaga gatattagag gcaagtaatc tacattacac cgaatttatg agtacagcaa
     3361 aagtctctac caatgcagct gccacaaagc aaataaaaca aataggtgca aatattgcca
     3421 aagcagtaga aacgtattta aaggaaaatg gtcgtgaagc tgatattgct agttttgctt
     3481 gggattttac cctcgttcag gataccacta taaatgcttt ttgcttgcca ggagggaaag
     3541 tcgtttttta tgaagggata ttacctattt gtgcaa
//
LOCUS       DAOPUR010000114         3575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_26538_length_3575_cov_3.226136, whole genome shotgun sequence.
ACCESSION   DAOPUR010000114 DAOPUR010000000
VERSION     DAOPUR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26538_length_3575_cov_3.226136"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PKN90_02840"
     CDS             complement(<1..781)
                     /locus_tag="PKN90_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ85248.1"
                     /translation="MKEKIPVTIVTGFLGAGKTSLLNMLIKQHPFTKFAIIENEFGEL
                     SVDGGLLATAKENIFELSNGCICCSLQSEFSTVIDRLLSSPYPFNHLLIETTGIANPA
                     TVIRPFLEGDKFPFLFELNAVVCLIDANNYKNKSNNFHEIHRQIAMSDTLIINKTDLI
                     TQEELNSLTKILHTKNPMATIFPNSYNSIDTRKILQAYSYREQQIASSIFSYTPNHTE
                     HKNDSQLITQPTTLFHTIESVAFEYETAFDVDTFSSWIRMFV"
     gene            924..1871
                     /gene="cysK"
                     /locus_tag="PKN90_02845"
     CDS             924..1871
                     /gene="cysK"
                     /locus_tag="PKN90_02845"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNZ85249.1"
                     /translation="MARIAKNLTELIGNTPLVQLSNYAEKRNLQANVIVKLELFNPAG
                     CVKERIALAMIEDAEADGKLKKGAEIIEPTSGNTGIGLAMVAAVKGYKLTLTMPETMS
                     IERRNLLKALGANLVLTPGALGMKGAIEKAAELHQANPFSYMPQQFDNPSNPSVHQRT
                     TGEEIWRDTNGKVDVFVGGVGTGGTISGVGAALKAHNPAIQIIAVEPADSPVISGGKP
                     GPHKIQGIGAGFIPKNYKPQVVDEVIQVSTDNAILTARQLAREEGMLVGILCGAAAYV
                     ATQVALRPENKGKNIVVLLPDTGERYLSTLLYAFEEYPL"
     gene            1993..3291
                     /locus_tag="PKN90_02850"
     CDS             1993..3291
                     /locus_tag="PKN90_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNZ85250.1"
                     /translation="MATQLKFETLQIHAGQEVDATTKSRAVPIYQTSSYVFDDAQDGA
                     DLFGLKKFGNIYTRLMNPTTDVFEKRIAALEGGVAALATSSGQSAQFLALNNILQAGD
                     NFVTTSHLYGGTYNQFRSQFKRLGVEVRFTENDEPATFEKYIDSNTKALYLETIGNPD
                     LNIPDFEAIAAVAKKYDIPLIVDNTFGAGGYLFRPLEHGASVVVESATKWIGGHGTSL
                     GGVIVDGGNFNWGNGKFPAFTEPSDSYHGLVFWDVFGANGPFGNIAFIIRARVEGLRD
                     WGNAISPFNSFLLLQGLETLSLRVERHVENALNLAQWLEKHPAVEYVNYPGLKSSKYH
                     SLANKYLKNGFGGVLSFKLKGDAEKADKLINNLKLISHLANVGDAKSLIIHPAATTHE
                     QLSPSEKLAAGVFPGLLRISVGIEHLEDIKADIEQALKAL"
BASE COUNT         1209 a          574 c          722 g         1070 t
ORIGIN      
        1 taacaaacat ccgaatccaa ctactaaatg tatccacgtc aaaggctgtt tcgtattcaa
       61 atgctaccga ttctatagta tgaaagagcg tagtaggttg tgtaatgagt tggctgtcgt
      121 ttttatgctc agtatggttt ggtgtatacg aaaatataga tgaagctatc tgttgttctc
      181 tgtaagaata ggcttgaagt atttttcttg tatcgataga gttatatgaa tttgggaaga
      241 tagttgccat tggatttttt gtatgcaaaa tctttgtcaa cgaattaagt tcttcttgtg
      301 taattaaatc agttttattg atgatgaggg tatccgacat ggctatttgt cgatgaattt
      361 catgaaaatt gttgctttta ttcttatagt tgtttgcatc aattaaacat actactgcgt
      421 ttaattcaaa caaaaatgga aatttgtctc cttctaaaaa aggtcttatt accgttgcag
      481 gattggctat gcctgttgtt tcgatgagta ggtggttaaa cggatacgga ctagaaagca
      541 atcgatcaat aacggtagaa aattctgatt gtaacgaaca acagatgcat ccgttgctca
      601 attcaaagat attctctttg gcagtagcta ataaacctcc atccacggat agttcgccaa
      661 attcgttttc gataattgca aatttggtaa acggatgttg tttgataagc atgtttagca
      721 aacttgtttt gccagctccc aaaaatccag taacaatggt aacaggtatt ttttctttca
      781 tacgtataaa taagcatgcg aaaatacata tttttgcttg atataccata cttgtggtat
      841 gtaaaatccc acacaaaagc tattgtgtat acatttatta ccagttatct ttgcatcagt
      901 taaacattaa aaaaatacat ggtatggcac gaatagcaaa aaacctgaca gaactcattg
      961 gaaatactcc attagtacaa ttatcaaatt atgccgagaa aagaaattta caggctaatg
     1021 taatcgtaaa actcgaatta tttaacccag caggttgcgt aaaagaacgc atcgctttag
     1081 ccatgataga agatgcagaa gccgatggaa aattaaaaaa aggagccgaa attattgaac
     1141 caacgagtgg aaatactggc ataggattag ctatggtagc tgctgtaaaa ggatacaaac
     1201 ttacgcttac catgcccgaa actatgagca ttgaaaggcg taatttatta aaagcattgg
     1261 gtgcaaattt ggtattaact ccaggtgctc taggaatgaa aggagctatt gaaaaagcgg
     1321 cagaattaca tcaagccaat ccattttcgt atatgccaca gcaatttgat aacccatcta
     1381 acccatcagt tcatcaacgt acaactggcg aagagatatg gagagatacc aatgggaaag
     1441 tagatgtttt tgtaggtggt gtaggcactg gaggaacaat cagtggtgta ggagctgctc
     1501 ttaaagcaca taatccagcc atacaaatta ttgcagttga acctgctgat tctcctgtaa
     1561 tttcaggtgg taaacctggt ccgcacaaaa tacaaggtat aggagcaggc tttattccaa
     1621 aaaattataa acctcaagta gttgatgagg tgatacaagt atccacagac aatgctattt
     1681 taacagcacg acaattggct cgagaagaag gtatgttagt aggtattttg tgtggagctg
     1741 ctgcttatgt agctactcaa gtggctttgc gtccagagaa taaaggaaaa aatattgtag
     1801 tattattacc agatactggc gaacgttatt tgtctacact tttatacgcc tttgaagagt
     1861 atccattata agaaattgaa aacaacatat agtatgagta atttatcaga agcttttcaa
     1921 caagcattag caaaaatact aaaacagcga gtaaaaaata caaatattga aaaacaaata
     1981 caagataaaa cgatggcaac acaactaaaa tttgaaacat tacagataca tgcaggtcaa
     2041 gaggtagacg caacaacaaa gtcaagagca gtacccatat atcaaactag ttcgtatgtg
     2101 tttgacgatg cccaagatgg ggcagaccta tttggattaa agaagtttgg taatatttat
     2161 acccgattaa tgaatcctac taccgatgtg tttgaaaaac gtatagcagc cctcgaagga
     2221 ggagtagcag ctttagcaac atcttcgggt cagtcagctc aatttttggc tttaaataat
     2281 atcttacaag ctggcgataa ttttgtaact acttctcatt tatatggagg tacgtacaat
     2341 cagtttaggt ctcagtttaa acgattagga gtagaagtgc gttttaccga aaacgacgaa
     2401 ccagctacat ttgagaaata cattgacagc aataccaaag ccttatattt agaaacaata
     2461 ggtaatccag atttgaatat tccagatttt gaggcaattg cagcagtagc gaaaaaatat
     2521 gatatcccct taatagtaga taatacattt ggagctggtg gttatttgtt tcgcccgcta
     2581 gaacatggag cttccgtagt agtcgaaagt gctactaaat ggattggcgg acatggcact
     2641 tcgttaggtg gtgtaattgt tgatggaggt aattttaact ggggaaatgg taagtttcct
     2701 gcttttacag agccatcaga tagttaccat ggattggtat tttgggatgt gtttggtgca
     2761 aacggaccat ttggaaacat agcgtttatc atcagagcac gtgtagaagg gcttcgagat
     2821 tggggaaatg caattagtcc attcaactcc tttttgctat tgcaaggact agaaaccctg
     2881 tcgttgagag tagaaagaca cgtagagaac gcgttaaact tagcacaatg gttagaaaaa
     2941 catccagcag ttgaatacgt aaattatcca ggtctcaaaa gtagcaaata ccattccttg
     3001 gctaacaaat atttaaaaaa tggttttggc ggtgtattgt cgttcaaatt aaaaggagat
     3061 gcagaaaaag cagataagtt aattaataat ttaaaactaa taagccatct agcaaatgtt
     3121 ggtgatgcca aatcattgat tattcatcca gctgccacta cccacgaaca actttcacct
     3181 tccgaaaagc tcgccgcagg cgttttccca ggcttgttgc gtatctctgt gggcatagaa
     3241 catcttgaag atataaaagc agatatagaa caagcattaa aagcattata agctattatt
     3301 ttgcgttata atgaaggctg tcttgagaaa agacagcttt tgttattttt aaaagtagag
     3361 ggctgtgcat tcttgtaaat acatacatac atgctactac tttactgtta agtatttgta
     3421 aaatacaatt atattttatt caatagttgt caacttgttt aggaaattgt atctttgcgg
     3481 agtttttatt aaaaaaatag caatcagaaa agaagtatgg aaaaagtaag ttatgccttg
     3541 ggtttgagta taggtaacaa cctaataggt agtgg
//
LOCUS       DAOPUR010000115         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_26638_length_3566_cov_3.109940, whole genome shotgun sequence.
ACCESSION   DAOPUR010000115 DAOPUR010000000
VERSION     DAOPUR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26638_length_3566_cov_3.109940"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PKN90_02855"
     CDS             <1..497
                     /locus_tag="PKN90_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNZ85251.1"
                     /translation="SIKKTQATLENLQISLVGDLKYGRAVHSLVMAMSHFNPSFHFVS
                     PPELQIPDSYKLLCNKQGISYKQYVEISEAINKADILYMTRVQRERFTDPVEYEKVKN
                     TYVLKNAMLENSKQNLRILHPLPRVNEIDMDVDTNPKAYYFEQARNGVYVRQAIICSI
                     LGLL"
     gene            520..984
                     /gene="pyrI"
                     /locus_tag="PKN90_02860"
     CDS             520..984
                     /gene="pyrI"
                     /locus_tag="PKN90_02860"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNZ85252.1"
                     /translation="MKTNINHELVVQALKNGTVIDHIPSDKLFQVVSILALHTMPNQI
                     TIGNNLESKKLGIKGIIKISDVFFTPKEVNKIALIAPNAKINIIKNYEVIKKVELVIP
                     DEIKDTLQCINPMCITNKEHVPTHFYFIDKKKRLAKCHYCERICKDEDLMLK"
     gene            986..1549
                     /locus_tag="PKN90_02865"
     CDS             986..1549
                     /locus_tag="PKN90_02865"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNZ85253.1"
                     /translation="MKQNWKPGTLIYPLPAVLVSCGSTEDEYNLLTISWTGTICSNPP
                     MCYISVRKERHSYPILVKNKAFVINLTTESMARATDWCGVRSGAKYDKWKEMNLTPEK
                     AQLVNAPIIKESPLNIECKVLEIKELGSHDMFIAEVVNVQADEQFMDAKTGEFKLSES
                     KLIAYSHGNYYKLGEKIGKFGWTVQKK"
     gene            1556..2203
                     /locus_tag="PKN90_02870"
     CDS             1556..2203
                     /locus_tag="PKN90_02870"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNZ85254.1"
                     /translation="MKKTLFLLITLLSASLYLSAQVDAKAIEILDKATKDIKSATFTN
                     TDFTMHINNAQNKASQKMQGNIRTHKEKMMINTAFAEILYDGKFQYVYLKEANELTIS
                     EANAEELKQFNPTYVLNTYKKNYRIPQPAIVMENNQQVYSIDLYPENKNETYFRINIK
                     IRIDKNQIVNISTFEKSGITTSIELTKMNVNASLLASDFIFDAKKYPKAEIIDLR"
     gene            2211..3158
                     /gene="trxB"
                     /locus_tag="PKN90_02875"
     CDS             2211..3158
                     /gene="trxB"
                     /locus_tag="PKN90_02875"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNZ85255.1"
                     /translation="MKTTETTRCLIIGSGPAGFTAAIYASRANLSPILYEGMQPGGQL
                     TTTTEVENFPGYPEGITGVEMMEDLKKQAIRFGTDVRWGSATEVDFSIRPFVVTIDGE
                     KTITADSVIIATGASAKYLGIPDEQKYAGSGVSACATCDGFFYRKKNVAVVGGGDTAC
                     EEAIYLSSICNKVYLIVRRNELRASKVMQKRVFETANIEVLFEHQTKSLFGEKVVEGA
                     ILWHKKGTPEEKEVSINIDGFFLAIGHTPNTRIFKNFIEMDEVGYIKTKNGSTKTNIE
                     GIFAAGDVADPVYRQAITSAGSGCKAAIDAEKYLHENGL"
     gene            complement(3208..>3566)
                     /locus_tag="PKN90_02880"
     CDS             complement(3208..>3566)
                     /locus_tag="PKN90_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85256.1"
                     /translation="FEDLPRYTKVAHVGLALLEHRGLSYYYALPNRIFDFIQADVPIL
                     ARNFPEMARIINGYGVGITIDANTPSLIAKTIQDIYNQQHQYTLSSFDKAKEDLCWEK
                     EQCILFEALKKSGLLN"
BASE COUNT         1363 a          579 c          626 g          998 t
ORIGIN      
        1 actccatcaa aaaaacgcaa gccacattgg aaaaccttca aataagtttg gttggcgacc
       61 taaaatatgg tagggctgta cattctttag taatggctat gagccatttt aacccttcct
      121 ttcactttgt ttcacctcca gagttacaaa taccagattc gtataaactg ctatgtaata
      181 aacaaggaat ttcttacaaa cagtatgtgg aaatatcgga agcaataaac aaggctgata
      241 ttttatatat gacgagagtg caacgtgaac gttttacaga ccctgtagaa tacgagaaag
      301 taaaaaacac atatgtacta aaaaatgcta tgttagaaaa cagtaaacaa aacttacgga
      361 ttttacaccc gctacctcgt gtaaacgaaa ttgatatgga tgtggataca aatccgaaag
      421 cgtattattt tgagcaagca cgcaacggag tatatgttcg tcaggcaatt atctgttcaa
      481 tattaggact tctttaggta aatcaataaa attatccgta tgaaaacaaa tatcaatcac
      541 gaattggtgg tacaggctct aaaaaatggg acagttattg atcatatacc atctgataaa
      601 ttatttcaag ttgtatctat actcgcccta catacaatgc ctaaccaaat aacgattgga
      661 aataatttgg aaagtaaaaa acttggtatt aagggtatca ttaaaatatc tgatgtattt
      721 tttaccccca aggaagtaaa taaaattgca ctaattgcac caaatgcaaa aattaatatc
      781 attaaaaatt acgaagttat caaaaaagta gaacttgtta ttcctgatga aattaaagat
      841 accttgcaat gtatcaaccc catgtgtata acgaacaaag agcatgtgcc cacacatttt
      901 tattttattg acaagaaaaa aagattagca aaatgtcatt attgcgaacg aatttgtaaa
      961 gatgaagatt taatgttgaa ataagatgaa acaaaattgg aaaccaggta cacttatcta
     1021 tccactacct gctgtactag ttagttgtgg cagtacagag gatgaatata accttttaac
     1081 tattagttgg acaggcacca tctgtagcaa tcctccgatg tgctatattt ctgtgcgaaa
     1141 agaacgacat tcgtatccta tattggtaaa aaataaggct tttgttatta acctaactac
     1201 cgaaagtatg gctcgtgcta ctgactggtg tggtgtaaga tcaggagcaa aatatgataa
     1261 gtggaaagaa atgaatctaa caccagagaa agcccaatta gtaaatgcac ctataataaa
     1321 agaatctcct ttgaatatag aatgtaaagt gctagaaata aaagagctag gatcccatga
     1381 tatgtttata gctgaagtgg taaatgtgca agctgatgaa caatttatgg acgctaaaac
     1441 aggagaattt aagctatccg aatcaaaact aattgcttat tcacatggta attactacaa
     1501 attaggcgaa aaaataggaa aatttggttg gacagttcaa aaaaaataat acacaatgaa
     1561 aaaaacactt tttctactca ttacattatt aagtgcatca ctgtatttat cagcacaagt
     1621 agatgccaaa gcaatagaga tactagataa ggcaacaaaa gatataaaat ctgcaacatt
     1681 tacaaataca gattttacca tgcatatcaa taatgctcaa aacaaagcat ctcaaaagat
     1741 gcaaggaaat atacgcacac ataaagagaa aatgatgatt aatacagcat ttgcagaaat
     1801 tctgtacgat ggtaaatttc agtatgttta tttaaaagag gcaaacgaac tcacaattag
     1861 tgaagccaat gcagaagaac taaagcaatt caatccaaca tatgtgctaa atacatacaa
     1921 aaaaaattat cgtataccac aaccagccat tgtgatggaa aataatcaac aagtatattc
     1981 aattgatttg tatcctgaaa ataaaaatga aacatacttt agaattaata taaaaatacg
     2041 tattgataaa aatcaaatag tgaatataag cacatttgaa aaatctggca taactaccag
     2101 tatcgaacta accaaaatga atgtaaatgc ttcgctttta gcatccgatt ttattttcga
     2161 tgcaaaaaaa tacccaaaag cagaaataat agatttaaga taatacagat atgaaaacga
     2221 cagaaacgac tcgttgccta attattgggt caggtccagc aggatttact gctgccattt
     2281 atgcttcaag agcaaattta tcacctattt tatatgaggg aatgcaacct ggtggacaat
     2341 tgacaacaac caccgaagta gaaaattttc caggttatcc agaaggaatt actggagtag
     2401 aaatgatgga agaccttaaa aaacaagcca tacgatttgg aacagatgta cggtggggct
     2461 ctgctaccga agttgatttt tcaatacgtc catttgtagt taccattgat ggagaaaaaa
     2521 caattacagc agattctgtc attatcgcaa caggggcatc agcaaaatat ttgggcatcc
     2581 ctgatgaaca aaaatatgct ggttctggtg tatctgcatg tgctacatgc gatggattct
     2641 tttatagaaa aaaaaatgta gcagtagttg gtggaggaga tactgcctgc gaagaagcta
     2701 tatacctatc atctatctgt aataaagtat atttgattgt tcgtagaaat gaactaagag
     2761 catcaaaggt aatgcaaaaa cgcgttttcg aaacggcaaa catcgaagtg ttattcgaac
     2821 atcaaacaaa atcacttttt ggtgagaaag tggtagaagg ggctatatta tggcacaaaa
     2881 aaggaactcc agaagaaaaa gaagtgagca ttaacattga tggattcttt ttagccatag
     2941 gtcacacacc aaatacaaga atcttcaaaa actttataga aatggacgaa gtaggctata
     3001 ttaaaactaa aaacggtagt actaaaacca atattgaagg gatatttgcc gctggggatg
     3061 tagccgaccc agtatacaga caagctatca cttctgctgg tagtgggtgt aaagcagcca
     3121 tagatgctga aaaatatcta cacgaaaacg gattataata gctaaatatg tatcatacaa
     3181 ccttatcaaa aatggtgagg ttgtagatta attcaataaa ccgctctttt taagtgcttc
     3241 gaataatata cattgctctt tttcccaaca taaatcctct tttgccttgt caaatgaact
     3301 caaagtgtat tggtgctgtt ggttatatat atcttgtata gttttagcaa taagtgatgg
     3361 agtgttagca tctatggtta ttccaacacc atatccatta ataattcgtg ccatttctgg
     3421 aaaattacgt gctaaaatag gtacatcagc ttgaataaaa tcaaatattc gatttggaag
     3481 ggcataataa taactcaaac ctctatgctc cagtaaggct aacccaacat gtgctacttt
     3541 cgtgtagcga ggtaaatctt caaaag
//
LOCUS       DAOPUR010000116         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_27043_length_3528_cov_3.283904, whole genome shotgun sequence.
ACCESSION   DAOPUR010000116 DAOPUR010000000
VERSION     DAOPUR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27043_length_3528_cov_3.283904"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKN90_02885"
     CDS             complement(<1..352)
                     /locus_tag="PKN90_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ85257.1"
                     /translation="MYLSQYIPFYKKNLKIAIPIILSQLGGGLVTVVDTIMVGHLGTV
                     ELAAASFANSIFLIGFVFSIGLTMAITPLVGKAYAEKKGKEIVELFQNSFLFVIIMAV
                     LIMIILLLAYFFMDY"
     gene            complement(460..1266)
                     /locus_tag="PKN90_02890"
     CDS             complement(460..1266)
                     /locus_tag="PKN90_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNZ85258.1"
                     /translation="MEVLVDKLMSSGFEIGKNVVIALVIFVVGRYLISLMNKLVRAMM
                     ERRQLDPAIRSFLGSMINIVLMVLLLISVVGALGVQTTSFAALLASAGIAVGMALSGN
                     MQNFAGGLMILFFKPYKIGDFIETQNVMGTVKEIQIFHTILTTPDNKIIFIPNGPLSN
                     GVLINFSHQLTRRIEWTFGVEYGTDLEKVKQILTSLIEKDSRILLEPTYLIALNKLAD
                     SSVNVVVRAWTKNEDYWSVYFEMNQSVYTTFNKEGINFPFPQLVVHHNKE"
     gene            complement(1357..2820)
                     /locus_tag="PKN90_02895"
     CDS             complement(1357..2820)
                     /locus_tag="PKN90_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNZ85259.1"
                     /translation="MNNQSSVNIKFASGEQLPLELHKVRIVQKLHLVPIERRLQALEE
                     GGYNTFQLGTLDVFFDMLTDSGTNAMSDNQLAAMMRADDAYAGSQSFVRLQKAVEEVL
                     GQKYLLPVHQGRAAENILGKAYVKKGSLVPMNYHFTTALAHITENGGSIAELLYPEAY
                     KIDSECLFKGNMNLELLEETIQKVGVENVPYIRMEASTNLIGGQPFSVQNLRDVKAIA
                     KKYGIRLVLDASLLGENAYLVKQREDEFKQKSMAEVILTMTSLADIIYFSARKLSSSR
                     GGGICTNNEAVYKELEPLVPLFEGFLTYGGISVREVEAMAVGLYETLDETMISQSPEF
                     IKYFVYDAKSKGIPMITPPGVLGAHVDAMSFCSHIPQTQYPAGALAAAFFLISGIRGM
                     ERGSVSNQRDENGNETYADMELLRLAIPRRVFTLSQIKYAIDRLAWLHKNRELIGGLK
                     FVYEPPVLRFFMGKLAPVSDWPQKLIAKFKEDFGDSL"
     gene            complement(2915..>3528)
                     /locus_tag="PKN90_02900"
     CDS             complement(2915..>3528)
                     /locus_tag="PKN90_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNZ85260.1"
                     /translation="YEATLQFFEELLLVLHPFMPFITEELWQSLYTRQDGESIVVQLQ
                     SKASEYDEELIVRFEKIKEIIAGIRTIRVQKNIPNKETLPLQIVGEHNSFFDLILLKL
                     GNLRSIEHVNEKTDTSVAFLVGTTEYAIVLGNLINIEDEIKKIETEIRYLEGFLQSVV
                     NKLSNERFVANAKPEVVDAERKKQLDAESKISTLREALQKFQA"
BASE COUNT         1186 a          683 c          488 g         1171 t
ORIGIN      
        1 tataatccat aaaaaaatag gcaagcaaca gtattatcat aattaaaaca gccataatga
       61 tgacaaaaag aaaagaattc tgaaacaatt caacaatttc ttttcccttt ttttctgcat
      121 aagcttttcc tactaatggg gttattgcca ttgttaaacc aatagaaaaa acaaagccaa
      181 ttaaaaaaat agaatttgca aatgaagccg cagctagttc tactgtacct aaatgaccca
      241 ccataatggt atcaactaca gttaccaatc ctcctccaag ttgtgaaaga atgataggaa
      301 ttgcaatttt taaatttttc ttataaaaag gaatatactg gctaagatac atatgtttat
      361 taattctgta atagaaacgc aaagttaatc attatactgc aaaaaaatag agagtagaaa
      421 cacaaccctc tatttttaca tattcctaac aaatacacct tactccttat tgtgatgaac
      481 aaccaattgt ggaaatggaa aattaattcc ttctttatta aatgtagtat atactgattg
      541 attcatttca aaatatactg accaataatc ttcgttctta gtccaagctc ttacaaccac
      601 attcacacta ctatcagcta atttatttag tgcaataaga tatgttggtt caagcaatat
      661 ccttgaatct ttttctatta aagaagttaa tatttgtttc actttttcaa ggtcagtacc
      721 atattccact ccaaatgtcc attcaattct acgtgttaac tgatggctaa aatttattag
      781 cactccattg cttagaggtc cgttaggaat aaaaattatc ttattatcag gagttgttag
      841 aatagtatga aaaatctgaa tttcttttac agtccccatt acattttggg tttcaataaa
      901 atcgccaatt ttgtaaggtt taaaaaataa tatcatcaat ccacctgcaa agttttgcat
      961 atttccgctc aaagccatac ccacagcaat acctgctgat gctaataaag ctgcaaaaga
     1021 ggttgtttgt acaccaagag cacctactac agatattagt aatagtacca tcaagacaat
     1081 attaatcata cttcccaaaa aactgcggat ggctgggtct aactgacgtc tttccatcat
     1141 ggctctcact aatttattca taagtgaaat taggtaacgt ccaacaacaa aaatgactag
     1201 tgctatcaca acattttttc caatttcaaa acctgaactc atcaatttgt ctactaaaac
     1261 ttccattctt tccataataa aaaaattaaa attgttatac aaaaaaaatc tactgaggaa
     1321 agaatcctca gtagacaaga tatcacaaca aacagcttat aaactatcgc caaaatcttc
     1381 cttaaatttt gcaatcagtt tttgaggcca atcagataca ggagctaatt tccccataaa
     1441 gaaacgtaaa actggtggtt catacacaaa ttttaatcca ccaattaact ctctattttt
     1501 gtgtaaccaa gccaatcggt caattgcata ttttatttga gacaaggtaa atactctacg
     1561 aggaatagcc aaacgtaata attccatatc agcatatgtt tcattgccat tttcatctcg
     1621 ttgattagac acagaccctc tctccatacc tctaatacca gagattaaga aaaatgcagc
     1681 ggctaaagct cccgcagggt attgtgtttg aggaatatgt gaacaaaaac tcattgcatc
     1741 tacgtgagcc cctaatactc caggaggagt aatcattggt atgcctttgc tctttgcatc
     1801 atacacaaaa tatttaataa attcaggact ttgcgaaatc attgtttcgt ctaatgtttc
     1861 gtataatcct actgccatag cctctacttc acgaacagaa atacctccat aggttaagaa
     1921 gccttcaaac aaaggcacta atggttcaag ctctttatat acagcttcgt tatttgtgca
     1981 aatacctcct cctcttgatg aacttaactt acgagccgag aaataaataa tatctgctaa
     2041 agatgtcata gtaagaataa cctcagccat tgatttttgt ttaaattcat cctctctttg
     2101 ttttaccaaa taagcattct ctcccaataa tgatgcatct aaaaccaatc gtataccata
     2161 tttttttgca attgctttta catctcgcaa attttgcaca gaaaaaggtt gccccccaat
     2221 taaattggta gaagcttcca tacgaatata aggcacattc tcaacaccta ctttttggat
     2281 ggtttcttct aataattcca aattcatatt tcctttaaat agacattcag aatcaatttt
     2341 ataggcttct ggataaagca actcagcaat tgaaccccca ttttctgtaa tatgtgctaa
     2401 tgctgtcgta aagtggtaat tcattggcac caatgatcct ttttttacgt atgcttttcc
     2461 taaaatattt tcggcagcac gaccttgatg cactggtaac aaatactttt gtcccaatac
     2521 ttcttcaacg gctttttgta aacgaacaaa actttgagag cctgcgtaag catcgtcagc
     2581 acgcatcatt gcggctaact gattatcgct catagcattc gtcccactat ctgtcaacat
     2641 gtcgaaaaaa acatctaaag ttcctaattg aaaagtattg tatcctccct cttcaagagc
     2701 ctgtaaacga cgttcaatgg gtactaaatg taatttttgt acgatacgca ctttgtgcaa
     2761 ttccaatggt aattgttcgc cactggcaaa tttaatgtta acagaagatt gattgttcat
     2821 gtgtgtaatt agtttaatag taattgatta tataaagttt ttttaagtac tacaaagata
     2881 ctaaaattat agaatatgcc ataaactata cattttatgc ctgaaatttt tgtaatgcct
     2941 cacgtaaagt ggagatttta ctttctgcgt ctagttgttt tttgcgttct gcgtctacca
     3001 cttcgggttt agcatttgct acaaatcttt cattgctaag tttattaact acagattgca
     3061 aaaatccttc taaatagcgt atttctgttt caattttttt gatttcatct tcaatattta
     3121 ttaaatttcc caacacaatg gcatattctg tagtgccaac cagaaaagcc acagaagtat
     3181 ctgttttttc atttacatgc tcaatagaac gtaaattgcc tagttttaga agtattaaat
     3241 caaaaaacga attgtgctca ccgacaattt gtaatggcaa ggtttctttg ttcgggatat
     3301 ttttttgcac acgaatagta cgaatgcctg caattatttc ttttattttt tcaaaacgta
     3361 caattaattc ttcatcatat tcggatgctt tcgattgtaa ttgtaccaca atactttctc
     3421 catcttgcct cgtgtataga gattgccaca attcctcagt aataaatggc ataaaaggat
     3481 gtaaaaccaa taataattct tcaaaaaatt gcaaagtagc ttcgtatg
//
LOCUS       DAOPUR010000117         3524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_27093_length_3524_cov_2.962813, whole genome shotgun sequence.
ACCESSION   DAOPUR010000117 DAOPUR010000000
VERSION     DAOPUR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27093_length_3524_cov_2.962813"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKN90_02905"
     CDS             <1..558
                     /locus_tag="PKN90_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNZ85261.1"
                     /translation="NEEVVAFFKDGQAGATPISYKVYADGTIDIPFISGIHVAGLTVR
                     EAAKKIELQVREFAPDATVKLGLSNDSFYLITEGKQGKFPMYKEKLNLFQALSLAGNI
                     PINADRQRVRIIRQKDAMSPPIIKEFDIRTESIIHSEFYYIQPNDLIYVSTIKGDFFK
                     VTDYSSTITSITSTLSFLLLIMGLF"
     gene            568..2967
                     /locus_tag="PKN90_02910"
     CDS             568..2967
                     /locus_tag="PKN90_02910"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNZ85262.1"
                     /translation="MENNVPQNNFNEENESSSFDIKIWIIRILKNWYLFFISISMFLT
                     VAYFQNKSWFPIYRSSALVIIEEANRGFNRSQQAFMEGFSVQGGYRNVNNQIVMFSSL
                     DLIKKVVDNLQDLHIEYYTRGRFTSTNLYKKSPVFIEKLFISPNGYEQEFFLEDVGNG
                     KDFLITYAGNKQIAEFTVKGEYGKQLETSLFFITIHKSDFYRNRFQLYFGFRDLDKLA
                     SMLHDRLTFAFKMEGSSVLEVSITGNVHERDCDFLNALCDAFLAEGLARKNDAAIKTI
                     EFIDSQMITISDSLRVSERALQNFKSDNFMVAANSSTELSAKYRALETKETELRLKDS
                     YIKYLTNYLEKNIEDGSLIAPTSLGVNEPTLTLLVKDFTEVMFKKKEVGERSPLYAKY
                     TRDLSNIKEQMFEALRNIRVGFNIEKADFDKRMAELQAQIQVLPIKEQQLLNFERKFK
                     VNDNYYSFLLQKRADAQILKASNSPDNIILDRARLTGVTNGASKGSNYSTFLLIGLLI
                     PLAFVGLKEFMYPFIRSENEILHLSRRAYQIIGVIRHTSKNIPVIVDAYPRSAMAEIF
                     RVVRTRMEFLNGKKKNMTVMFTSTHSGEGKTYVSVNMAAIYAMTGKKTILIDSDLRKP
                     SVSATLSLPENKGLSNYLAGQIDLSEAIIKKSNYKFDIMTVGVIPPNPGELMKSQKMK
                     ELIKELKEIYDFIIFDAPPIGLVADSYAIINEMDLNIYVVRCERTNKPLFKKTITQLQ
                     EDKVPNVHVLFNDVNIRKLEYATSYSNYSKYGKSYYTNLLYKNYKDNNGVYFDDLEDD
                     V"
     gene            3024..>3524
                     /locus_tag="PKN90_02915"
     CDS             3024..>3524
                     /locus_tag="PKN90_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HNZ85263.1"
                     /translation="MNVQDKKIAVIGLGYVGLPLAIEFGKKYRVLGFDIKAARIEELQ
                     AGEDSTLEADLEGMKIATDLYKKNEQIGLSFSSIQEDLKNFNTFIVTVPTPIDEFNNP
                     VLTPLISASRMIGNVLKKGDLVIYESTVYPGCTEEDCVPVLEKQSGLKFNKDFFCGYS
                     PERINPG"
BASE COUNT         1237 a          515 c          617 g         1155 t
ORIGIN      
        1 aacgaagagg tagttgcttt ttttaaagat ggacaagcag gtgctactcc tatttcttac
       61 aaagtatatg ctgatggaac cattgatatt ccgtttatct ctggtattca tgtagcaggg
      121 ttgacggtaa gagaagctgc aaaaaaaatt gagctacaag ttcgtgaatt tgcaccagat
      181 gctaccgtaa agttaggttt atcaaatgac tctttttatc tcataactga agggaaacaa
      241 ggcaaatttc ccatgtacaa agaaaaacta aacttgtttc aagccttatc attagccgga
      301 aatataccta taaatgccga cagacaacgt gtgaggataa ttcgtcaaaa agatgccatg
      361 tctccaccca tcatcaagga atttgatatt cgtacagaaa gcatcatcca ttccgaattt
      421 tattacattc agccaaacga tctaatttat gtttctacaa taaaaggaga tttttttaaa
      481 gtaaccgatt attcatctac aattaccagt attaccagta cgttgtcttt tttactgcta
      541 atcatgggat tattttaata tatttttatg gaaaacaacg tacctcaaaa taattttaat
      601 gaagaaaatg aaagttcttc ttttgatatt aaaatatgga ttattcggat tttaaaaaac
      661 tggtatcttt ttttcatatc tatcagtatg tttcttactg tagcttattt tcaaaataaa
      721 agctggtttc ctatttatcg ttcgtcagct ttggttatta ttgaagaagc aaatagaggt
      781 ttcaacagga gccaacaagc ttttatggaa ggattcagtg tacaaggagg atatagaaat
      841 gtaaacaatc aaattgtaat gtttagttct ttggacttaa tcaaaaaggt agtagataat
      901 ttacaagatt tacatataga atactataca cgaggacgtt ttacttcaac aaatttatac
      961 aaaaaatctc ctgttttcat tgaaaagtta tttatatcgc ctaatggata tgaacaagaa
     1021 ttctttttag aggatgtagg aaatggaaaa gattttttaa taacatacgc tggcaataaa
     1081 caaattgcag aatttacagt taaaggagaa tatggaaaac agctagaaac gtctcttttc
     1141 tttattacta ttcataagtc tgatttttat cgtaaccgct ttcaattgta ttttggtttt
     1201 agggatttag ataaattagc ttctatgcta catgatcgct taacatttgc ctttaagatg
     1261 gagggctctt cagtattaga agtatccatt actggcaacg tgcatgaacg ggattgtgat
     1321 tttctaaatg ctttgtgtga tgcgttttta gcagaaggct tagctcgaaa aaatgatgca
     1381 gcaataaaaa caattgaatt tattgattca caaatgatta cgatatcaga ttcgttgcgt
     1441 gtctcagaaa gagcattgca aaactttaaa tcagataatt ttatggtagc agctaattca
     1501 agtactgaat tgtctgccaa atatcgagct ttagaaacaa aagaaacaga attacgttta
     1561 aaggactcct atattaaata cttaacgaat tatcttgaaa agaacattga ggatggctcc
     1621 ctgattgctc ctacaagttt aggggtaaat gaacctacat taacattact agttaaagat
     1681 tttactgaag taatgttcaa gaagaaagag gtaggagaaa gaagtccctt gtatgctaag
     1741 tatactcgtg atttatcgaa tataaaagag cagatgtttg aagcattacg caatatacgt
     1801 gttggtttta atattgaaaa agctgatttt gacaaacgaa tggctgagtt gcaggctcaa
     1861 atacaagtat taccaataaa agaacaacaa ttactcaatt ttgagcgtaa gtttaaggta
     1921 aacgataatt attattcgtt tttattacaa aaaagagcag atgcacaaat actaaaagca
     1981 tcgaattctc ctgataatat tattttagat agagctcgct taacaggtgt tacaaatggt
     2041 gccagtaaag ggagtaatta tagtactttt ttattaatcg gacttctaat tccattagca
     2101 tttgttgggc taaaagaatt tatgtatccc tttattcgtt ctgaaaatga gattttgcat
     2161 ctttctcgca gggcatacca aattattggg gttattcgtc atacctcaaa aaacatacct
     2221 gtaattgtag atgcctatcc acgttctgct atggcagaga tatttagagt agtgcgtaca
     2281 cgtatggaat ttttaaatgg taagaaaaaa aacatgactg taatgtttac ttcaactcat
     2341 tcaggagaag gtaaaacata tgtatctgta aatatggctg caatatatgc tatgacaggc
     2401 aaaaaaacta tcttgattga ttccgatttg cgtaaaccta gtgtttctgc taccttatca
     2461 ttgccagaaa ataaggggtt gagtaattat ttagcgggac agattgattt gtcagaggct
     2521 attataaaaa aatcaaatta caagtttgat attatgacgg taggagtcat tcctccaaat
     2581 ccaggagagt taatgaaatc gcaaaaaatg aaagaattaa tcaaagaatt aaaagaaatt
     2641 tatgatttta ttatatttga tgctcctcct attggtttag tagccgattc ctatgctatt
     2701 ataaatgaaa tggatttaaa catttatgta gtgcgttgtg agcgaaccaa taagccctta
     2761 tttaaaaaga caattacgca attgcaagaa gataaagtac cgaatgtcca tgttttattt
     2821 aatgatgtaa atataagaaa attagaatac gctacttcat atagtaacta ttctaaatac
     2881 ggcaagtctt actataccaa tttattatac aaaaactaca aagataataa cggtgtttat
     2941 tttgatgatt tagaagatga tgtttaattg acacttatca ctaaacattc accactaatc
     3001 actaatcact aatcattatt aacatgaatg tacaagacaa aaaaatcgca gtaataggct
     3061 tagggtacgt tggacttcct ttggctattg aatttggtaa aaaataccgt gttcttggat
     3121 ttgatattaa agcagcacgt attgaagagt tacaagctgg agaagattct actttggaag
     3181 cggatttgga gggaatgaaa atagccactg atttgtataa aaaaaatgaa caaattggct
     3241 tgtctttttc atctattcaa gaagatttaa aaaattttaa cacctttatt gtaaccgtac
     3301 caactcccat tgatgaattt aataatccag tgttaacacc ccttatttca gcatctcgta
     3361 tgattggtaa tgtgttaaaa aagggagatt tagtgatcta tgaatcaact gtataccctg
     3421 gttgtacaga agaagattgt gtgcctgtgt tagaaaaaca atcaggttta aaattcaata
     3481 aggatttctt ttgtggctat tcgcccgaac gtatcaatcc gggt
//
LOCUS       DAOPUR010000118         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_27235_length_3510_cov_3.219682, whole genome shotgun sequence.
ACCESSION   DAOPUR010000118 DAOPUR010000000
VERSION     DAOPUR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27235_length_3510_cov_3.219682"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1106
                     /gene="mltG"
                     /locus_tag="PKN90_02920"
     CDS             69..1106
                     /gene="mltG"
                     /locus_tag="PKN90_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNZ85264.1"
                     /translation="MNYLRNHKTLLILVAVIGITASGLMLNAFILPNLNGNGNKKQYL
                     FVYPEQTWSDVINTLTEMEVVNSTFSLKLAIALKYADMPPKTGKYDIEPNLSNRYLLN
                     RIAHGLQSKIILRVVSTRNTQQVARNLSRQLMVDSTSLMKQFHNPTLLEKYELTPSNS
                     LCLFLPFNAEITWDTPIEEIIQLIEKEYTTFWNDNRKQLAKKINLTPSEIHILASIVE
                     EETNNFEDKKMVAGLYINRLRKDMLLQADPTVRYATGNFSLNRILKRHLAINSPFNTY
                     IYKGLPPAPIRIPTQESIDAVLNYTKHPYVYMVAKSDFSGTHNFSRTFDEHLRKARAY
                     QKQLNKRGIFE"
     gene            complement(1113..2453)
                     /locus_tag="PKN90_02925"
     CDS             complement(1113..2453)
                     /locus_tag="PKN90_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNZ85265.1"
                     /translation="MARLTTLIIFFKSALHFLRFDIWRITDGEVTAAKNRIYTIIKVF
                     VISVRRFFEDKIFAKSAALTYYTLLSVVPVLALVIAIGKGFGVQYYLENQFVAIFPSH
                     STVLKEAFFLVDSYIQQIQGGVFFGIGFVLLFWSVLNMFSEIEIAFNDIWQIKKLRNT
                     FRRFADYFSMMLILPVLLIVSSGLSIYLNGTIQDTVLSSLIVPVWYGFLKILPFIVNI
                     VLFTLMYLFFPNTKVRFIAALFAGVVAGVLFQLFQMLYISGQIWISYYNAIYGSFAAL
                     PLLLLWLWASWSIILYGAEFAFSVQNIKNYEFESDVKNISRRYENFLFVLISSVIVKR
                     FAEKLPAMNAEELSTHYNIPIRLVNRIVSKLLDAGIIVESISTVKKTEEIVYQPAIDI
                     QHLTLAYLFEQIDGLGSENFKIDITHEHAQSWQATLFVQNSTQSYASSVLLKDL"
     gene            complement(2472..2832)
                     /gene="rnpB"
                     /locus_tag="PKN90_02930"
     ncRNA           complement(2472..2832)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKN90_02930"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3015..>3510
                     /locus_tag="PKN90_02935"
     CDS             3015..>3510
                     /locus_tag="PKN90_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNZ85266.1"
                     /translation="MRQLKIVKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIK
                     KGDRIALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERRPSPEELAE
                     ELDIP"
BASE COUNT         1318 a          665 c          546 g          981 t
ORIGIN      
        1 gatattttca aacattctta ttcaatattt ataaaaaagc aacatatttg taaaaaacaa
       61 aaaagaaaat gaactattta cgtaaccata aaacactact tatactagta gcagtaatag
      121 gaataacagc tagtggttta atgcttaatg cctttattct ccctaattta aatggtaatg
      181 gtaacaaaaa acagtatctc tttgtgtatc ctgaacaaac gtggagtgat gtaataaaca
      241 cgttaacaga aatggaggtt gttaatagta cattttcttt gaaacttgcg attgctctga
      301 aatatgctga tatgccccca aaaacaggaa aatatgacat agagcccaat ctatctaaca
      361 ggtatctact gaatagaatt gctcacggat tacaaagcaa aattattctt agggtggttt
      421 ctacccgtaa tactcaacaa gttgcacgta atcttagtcg ccaattaatg gttgactcaa
      481 cttcgttaat gaaacaattt cacaatccta ctttattaga aaaatacgaa cttactccga
      541 gtaattcatt gtgtttgttc ttacctttta atgcagaaat tacatgggat acgccaatcg
      601 aagaaattat acaattgata gaaaaagaat atacaacgtt ttggaatgat aacagaaaac
      661 aattagccaa gaaaataaat ttaacaccat ctgagattca cattttagct tctatagtag
      721 aagaagagac aaataatttt gaagataaga aaatggtagc aggtctctat atcaatagat
      781 taagaaaaga catgttgtta caagcagacc ctactgtgcg atatgctacg ggcaattttt
      841 ctcttaatcg gatactaaaa cgacaccttg ctatcaactc tccattcaat acatatatct
      901 acaaaggact acctcctgct cctattcgta ttcctaccca agaaagtatt gatgctgtac
      961 tcaattacac caaacaccca tatgtatata tggtcgcaaa aagtgatttt tctggtacac
     1021 acaactttag tcgcacattc gacgaacatt tgcggaaagc tagagcatac caaaagcaat
     1081 tgaacaaacg tggtattttt gaataaagtc tgttataaat cttttaataa tacagaagaa
     1141 gcataggact gtgtgctatt ttgaacaaat aaggtagctt gccaagattg ggcatgctca
     1201 tgtgtaatgt caattttaaa attctcagac cccaatccat ctatttgctc aaataaataa
     1261 gcaagcgtta aatgttggat atctatagct ggctgataaa caatttcctc cgtttttttt
     1321 acagtagaaa tactctcaac aataatccct gcatctagta acttagatac aatgcgattg
     1381 actaatcgta ttggaatatt atagtgagta gataattctt ctgcattcat tgcaggaagc
     1441 ttttcggcaa aacgcttaac aatgacagaa ctgataagaa caaacaaaaa gttttcgtaa
     1501 cgcctagata tatttttaac atcagactca aattcgtaat ttttaatatt ttgcacagaa
     1561 aaagcaaatt ctgcaccata taaaatgata gaccacgacg cccataacca taacaaaagc
     1621 aatggcaatg ctgcaaaact accgtaaatg gcattataat aagatatcca aatttgtccg
     1681 ctaatataca acatttggaa taactgaaac aatacaccag cgactactcc cgcaaataaa
     1741 gcggcaataa acctcacttt tgtatttgga aaaaataaat acatcaatgt aaacaaaaca
     1801 atattaacaa taaaaggtaa tattttgaga aaaccatacc atacaggcac aatcaacgaa
     1861 gaaagcaccg tatcttgaat agtcccgttc aaataaattg acaacccact agatacaatg
     1921 agtaacacag gaagtattaa catcatggag aaataatcgg caaaacgacg aaaagtattt
     1981 cgcaattttt ttatctgcca aatatcattg aaagcaatct caatttcgga aaacatgtta
     2041 agtacagacc aaaacaacaa tacaaaacca attccaaaaa acacaccacc ctgaatttgc
     2101 tgaatatatg aatcgactaa gaaaaaggct tctttcaaaa cagtactatg gcttggaaat
     2161 atcgcaacga attgattctc aagataatat tgcacaccaa agcccttacc aatcgcaatt
     2221 actaaagcaa gtacaggaac aacagaaagc aaggtataat aggtcagagc cgccgacttt
     2281 gcaaatattt tatcttcaaa aaaacgacga acagaaataa caaatacttt tataatggta
     2341 taaatacgat ttttagctgc cgtaacttcc ccatccgtta tccgccaaat gtcgaaacgt
     2401 aaaaaatgta aggctgattt aaaaaaaata atcaatgtag taagtctagc catagcatac
     2461 aagtattgta aaaaaatagc aggtctataa gccgggttct gtatttactt gcgtaaatgt
     2521 ttgtcattta tctacaacaa caatcacttg ttgcctctag caacctaccc cccgacatcg
     2581 gacgagcagc cctacatgcg tcggtataca tggtcttgca acccataagg cgtacggctc
     2641 tgcttgttac caaacaaacc ggtgagctct tacctcacct tttcaccctt accacacaca
     2701 aggcatggcg gttattttct gttacactac tttgctctca cgaacaactt cccgttaaga
     2761 agtatggtgc tctacgttgc ccggactttc ctcctttcac acacaatgaa cggcgacaaa
     2821 ccgacctact atctgaatac aaagatacgc aattatgtta ttgaaacaac actataatga
     2881 aaatgaaata gaaaattggg atagaaaaac taaatgagat aacataaaat aacatataga
     2941 agaagaatga gtacactatt ttttcgttaa ttcgtttttt tattctatct ttgcaaacta
     3001 attttgcccg cattatgaga caactgaaaa tcgtaaaatc aatcactaat agagaaagcg
     3061 cttcgctgga caaatattta caagaaattg gacgcgaaga ccttattaca gtagaagaag
     3121 aagttgagct agctcaacgc ataaaaaaag gagatagaat tgccctcgaa aaacttacta
     3181 gagccaattt acgatttgtt gtatctgtag caaaacaata tcaaaatcaa ggtcttagcc
     3241 taccagattt aatcaatgaa ggaaatttag gattaatcaa agctgctgaa aaatttgatg
     3301 aaacccgtgg ttttaagttc atttcgtacg ctgtatggtg gattcgtcaa tcaatattac
     3361 aagccttagc agaacagtct cgtattgtac gtttaccact aaatcaagta ggatcattaa
     3421 ataaaatcaa caaagctttt tccaaatttg aacaagaaaa tgaacgtcgt ccttcgccag
     3481 aggaattggc tgaagagttg gatatacctg
//
LOCUS       DAOPUR010000119         3489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_27460_length_3489_cov_3.258882, whole genome shotgun sequence.
ACCESSION   DAOPUR010000119 DAOPUR010000000
VERSION     DAOPUR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27460_length_3489_cov_3.258882"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..980)
                     /gene="prmA"
                     /locus_tag="PKN90_02940"
     CDS             complement(135..980)
                     /gene="prmA"
                     /locus_tag="PKN90_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNZ85267.1"
                     /translation="MINYLRFAIKMNPFSEYLADILAYELGAIGFESFELQEDGVSAF
                     IQEPDWKGALFSEVIHDVAKLYETQISFKKERIVQQNWNEEWEKHYFKPILVDDVCLI
                     RSSFHTNLPNAKYDIVIDPKMSFGTGHHETTSLMLRWILEEVLVDKAVLDMGCGTAVL
                     AILAKMRGANKVWAVDIDEWCYVNAKENILVNAVDTIQVFQGGAEVVDNEKFDVIFAN
                     INLNVLLEYMPQYVACLSKNGILFMSGFYLSDLSHIHDLAIKLGLRQIGYKEKNNWIA
                     VKYCL"
     gene            1136..2167
                     /locus_tag="PKN90_02945"
     CDS             1136..2167
                     /locus_tag="PKN90_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85268.1"
                     /translation="MKKKALLISSLFIAFSMSMFAQYDDIYYNPRKANETRITTSNEE
                     TVVISNNVRDVDTYNRQAPANRPNQTSNVEQDDFEYTKRITRFHNPEAVTINNPEYVY
                     IYNDTPSEPNPTINIRVTQRWNPWDYSYFTRYYYPSYHWSWDSNYGWGYTYNTYYPFY
                     STYPSSFYYGYYSPGYGYYGYGYYGYPSYYHHRPHYYHHPYHHQPTYTTRKGYNNYDR
                     TPNYVNDGRGSSSSYGYQNSGQRSTRSVGTNPTNTNASSTRNTSTYNNRERTFNSAPS
                     SSTQNTRKSDVTTNTSTPRETSSGRSFNSSPSSSNSNNSGTRSTQSSDNSSSSNGGSR
                     SNGGRSFSR"
     gene            2197..>3489
                     /locus_tag="PKN90_02950"
     CDS             2197..>3489
                     /locus_tag="PKN90_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85269.1"
                     /translation="MKKIIITSLCALGIMSFANAQFQSEYDALKVAQTDLNGTARFMS
                     VAGAMGALGGDASTLFYNPAGIATYRSSELTFTGNLNWNNTSALGSMASRFLFNLNNV
                     SYISAYKTGSENGLVAFNWGFAYNRAKTFDKNIRYSTNSNLSLGDFLANYTEISGNAA
                     GYTASVFDEDNDVYRDQRVPWASVLAWDGMMIANPQDESNNSINNQYESYRNNLISQS
                     IGGGVQNTYTIAERGSVNEYAFSGGANISNILQLGVSFVLLNLDYQMESIFEEKFENN
                     STYKYTSLYQTDGSGFSIKVGAIARPTQWLRIGGAFHSPSWYYIKDNNEGSIGSKIVT
                     EVEPDYNGSTPVGYGSYYLNTPMKGLANLGFVIGNYGFIGVDYEYTDYSTMKMKNNDI
                     INSYMVDQNNKIATNFDATHTIRVGTELRPIENVSVRLG"
BASE COUNT         1213 a          655 c          546 g         1075 t
ORIGIN      
        1 ctctaaccat ctgagctatc gcgccatcat tttttagtgg tgcaaaggta atactttttg
       61 ggaatttcaa aaaataaaat agcatttttt caatctcaac tacttaatat ttccccctat
      121 actttgtata ttgcttataa acaatatttt accgcaatcc aattattttt ttctttgtat
      181 ccaatttgcc gtaaaccaag cttaatggca agatcatgta tgtgagacaa atctgataga
      241 taaaaaccac tcataaataa tatcccattt tttgataaac aagcaacata ttgaggcata
      301 tattcaagta aaacattcag gtttatattt gcaaaaatca catcaaattt ctcattatca
      361 actacttctg ccccaccttg aaacacttgt atggtatcaa ctgcatttac caaaatattc
      421 tcttttgcgt ttacataaca ccattcgtca atatcaacag cccacacctt atttgcacct
      481 ctcatttttg ctaaaatggc taatacagct gtaccacaac ccatatccaa cactgcctta
      541 tccactaata cctcttctaa aatccatctt agcatcaaac tagttgtttc gtgatgacct
      601 gtaccaaaag acatcttagg atctattaca atatcgtatt ttgcattggg caaattagta
      661 tgaaaagatg atcgtataag acatacatca tctactaata taggcttaaa ataatgcttt
      721 tcccactctt cattccaatt ttgttgtaca atacgttctt ttttaaagct aatttgtgtt
      781 tcatataact tagcaacatc atgaattact tcactgaaaa gagctccctt ccaatcaggc
      841 tcttgaataa acgcagatac accatcttct tgcaattcaa aactctcaaa cccaatagca
      901 ccaagctcat acgcaagaat atcagccaga tattcggaaa aaggattcat tttgatggca
      961 aatcttaaat aatttatcat atattagaat ttaataataa aatctttcat ttattaattt
     1021 tttgttaaca aatgaatgtg tatttttgta tcatagaatc gtacatacac acaacacaca
     1081 ttttcacgca tataaaagca acaaaagtac aaaaaaatcc ttaaattgaa atgccatgaa
     1141 aaagaaagct ttacttatca gtagtctgtt tattgcattt tctatgtcga tgtttgctca
     1201 atacgacgac atctactata atccaagaaa agcaaatgaa acacgaataa ctacaagtaa
     1261 cgaagaaact gtagttattt ctaataatgt gcgtgatgtt gatacttata accgtcaggc
     1321 tccagcaaac cgaccaaatc aaacatcgaa tgtagaacaa gatgattttg agtatacaaa
     1381 aagaataaca cgttttcata acccagaagc cgttactatc aacaacccag aatatgtgta
     1441 catatacaac gacacaccct ctgaacccaa tccaactata aatattcgtg ttacacaacg
     1501 ctggaatcca tgggattata gttattttac acgttattat tatccatcct atcattggtc
     1561 atgggattca aactatggat ggggttatac atacaacacc tactatccat tttatagtac
     1621 atatccttca tcattttatt atggttatta ttctccaggt tatggttatt atggttatgg
     1681 gtactatggt tacccatctt attatcatca tagaccccac tactatcacc atccctatca
     1741 tcatcaacct acttacacaa caagaaaggg ctataacaat tacgaccgca cacccaatta
     1801 tgtaaatgat ggcagaggca gtagctcttc atatggatat caaaattctg gacaacgttc
     1861 aacacgttct gttggaacaa acccaacaaa tactaacgca tctagtacac gtaatacatc
     1921 aacttacaat aatagagagc gtacatttaa tagtgcacca tcttcttcta cacaaaatac
     1981 cagaaaaagt gacgtaacaa cgaatacctc tacaccaaga gaaacatcta gcggtagaag
     2041 tttcaattcc tctccatcat cgtctaatag caataattct ggtacaagaa gtacgcaaag
     2101 cagtgataat tcatcgtctt ccaacggagg aagcagaagc aatggaggtc gctcgtttag
     2161 cagatagtaa ttatgaaata aataaaaaat aatacgatga aaaaaataat aattacaagt
     2221 ttgtgtgcat taggaattat gagctttgca aatgctcaat ttcagtcgga atacgatgca
     2281 ctaaaagtag ctcaaactga cttaaatggg acagcacgtt ttatgtctgt ggcaggagcg
     2341 atgggagctt taggtggaga tgcctctacc ctattttaca atccagctgg gattgctacg
     2401 taccgcagtt ctgagctcac ctttactgga aacttgaatt ggaacaatac ctcagcttta
     2461 ggcagtatgg ctagccgttt tttattcaat ttgaataatg tatcttatat cagtgcatat
     2521 aaaacaggat ctgagaatgg cttagtggct tttaattggg gttttgctta taatagagca
     2581 aaaacttttg ataaaaacat tcgttattca actaatagta atttatcatt aggcgacttc
     2641 ttagccaatt atactgaaat ttctggaaat gcagcaggct atactgctag tgtttttgat
     2701 gaagataatg atgtatatag agaccaacgt gtgccatggg cttcggtatt agcatgggat
     2761 ggcatgatga ttgcaaaccc acaagatgaa tcgaataatt ctatcaataa ccaatacgaa
     2821 tcatatagaa ataatttaat aagtcaaagt attggaggtg gagttcagaa tacgtatacc
     2881 atagctgaac gtggatcagt aaatgagtat gcatttagtg gaggtgcaaa tataagtaat
     2941 atactgcaac tgggagtaag ttttgtgtta ctcaatctgg attatcaaat ggaatcaatt
     3001 tttgaagaaa aatttgaaaa taatagcaca tacaaatata caagtttata tcaaactgat
     3061 ggttctggct ttagtattaa agtaggtgct attgcccgac caacgcagtg gcttcgtatt
     3121 ggtggtgcat tccactcccc ttcttggtat tatataaaag acaataacga aggatcaatt
     3181 ggaagtaaaa ttgtaaccga agtagagcct gactacaacg gatcaactcc tgtgggctat
     3241 ggttcttact acctaaatac accgatgaaa ggactagcta accttggttt tgtaattgga
     3301 aattatggat ttattggagt agattacgag tataccgact attcaacaat gaaaatgaaa
     3361 aataatgata ttatcaattc ctatatggtg gatcagaata ataaaattgc taccaatttt
     3421 gatgctacac ataccattcg tgttggtact gaacttagac caattgaaaa cgtgtctgta
     3481 agacttgga
//
LOCUS       DAOPUR010000120         3488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_27463_length_3488_cov_4.879406, whole genome shotgun sequence.
ACCESSION   DAOPUR010000120 DAOPUR010000000
VERSION     DAOPUR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27463_length_3488_cov_4.879406"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PKN90_02955"
     CDS             <1..658
                     /locus_tag="PKN90_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85270.1"
                     /translation="KKGLNIPNTFELLDELVKSLQIKNKFRLLFRIGVNRRDGVHELI
                     TMARTFNTYHRKYKWIEAIDINGDERTFPLEKLAPILKSLKQEEIPFTIHAGEGSDLT
                     NSLEDALKCDPIRIGHGISAINSTDIMKELCIRNIPLEICITSNIATSNSPQSPNKHP
                     IKTLFNNKVPILLGSDNPTFLDTTIKREYELATKVCGPDFLSVLDSNIEQTLEKKDEG
                     "
     gene            complement(671..1546)
                     /locus_tag="PKN90_02960"
     CDS             complement(671..1546)
                     /locus_tag="PKN90_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85271.1"
                     /translation="MRLVNAGLKIDVITSNSSKLFCDKEQFLYEYIPDLTIDSLPNGG
                     KPDFIIELNVSKSNSFDIIDNVIMIRYSEDCSIKDVVTLLEYGLEYCRQSKGVYCLHG
                     SAVTKNQRAIVFVGQVSGIGKTSISLELALKHGYLFLGDEKILLRYINNQPFIKSTNR
                     LKFNKPYLGDYFRINEADFINRFGNVTKNWIPLTLLVQPQICPKGALLYEKFNHTKTE
                     FHLYEEITRKIRGCSRRVKNFTEPLLSIDDYALSQKRSSFCKELSLVVDSYHVVGDKS
                     LVASFLDDLLNKGDA"
     gene            complement(1533..2651)
                     /locus_tag="PKN90_02965"
     CDS             complement(1533..2651)
                     /locus_tag="PKN90_02965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ85272.1"
                     /translation="MKKNILVFLPTASIIVGGGEIAPLSQSQALSKNGHSVTILTVRT
                     EEPTSYYQDFKRRNPQIKFVEVTSPIFGCSYANFINSHENIHLLYLSLYDWYKTITDN
                     FDVCLIHYAPGMFCIPHIKRKILFLHGTPSEYSVYNDLAVTLTDNVIAVSNSIKNEWK
                     NSLLNISKNKDIIVINNGIDLYPYENVKRVDNQIFFIGRLIDIKGLEVLIESIALLIN
                     TYPYIKLYIAGTGTIEYTNLLKNLVNKLDLNENIFFLGHISEEQKYKLYRESSFCVFP
                     SFAKEGVLTTMLEASTQYCPTITTDSCGMVDFIIDEKYGLLAKPRDKVSLAEKIELFL
                     TDSTLRLNCGNNAYNRAKTEFTWESNVDKLNKIFDEAS"
     gene            complement(2653..>3488)
                     /locus_tag="PKN90_02970"
     CDS             complement(2653..>3488)
                     /locus_tag="PKN90_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ85273.1"
                     /translation="IIPVYGGFESLKKTVLSIQCQSFVHLAKQKIQVVIVNDGSESKL
                     TKSMLNSDDLDINIINIEKNLGRVYARNVGVSVAKFNKLIFLDSDVVLPSNYVYEMYI
                     RLCLIPNAVLISFKECLDPSNPELENQNIIKGIRKPSNFQCDWRVNCYINEAKKGLRK
                     INESNFRNIVMETNGLKNFGFNRFIGIFDLAATVATHNVAMSKSLYKAVDGFDTDFTG
                     WGLEDTHFGYKLIASGAMIIPVVSTGIFHIDHPPRSGSEEEKWNEFAKNLETLKNKLN
                     E"
BASE COUNT         1205 a          673 c          468 g         1142 t
ORIGIN      
        1 caaaaaaggt cttaacatac ccaatacttt tgagttactt gatgaattgg taaaatcact
       61 acaaattaaa aacaaatttc gtttactctt tagaatagga gtcaacagac gcgatggtgt
      121 tcatgagtta ataactatgg caagaacatt taatacttac caccgcaaat acaagtggat
      181 agaggcaatt gatataaacg gagatgagcg tacatttcca ttagaaaagt tagctccaat
      241 tttaaagtcc ctcaaacaag aagaaatacc ttttactata catgcaggag aaggtagtga
      301 cctaacaaat tccttagaag atgctttgaa gtgtgatcct ataagaattg gtcatggaat
      361 ttcagcaatt aacagtactg atattatgaa agagttatgt atacgcaaca ttcctttaga
      421 aatctgtatc actagcaaca tcgcaacttc caacagtccc caatccccca acaaacaccc
      481 aatcaaaacc ctctttaaca ataaggtccc aattctacta ggatctgaca accctacctt
      541 cctagatacc acaataaaaa gagaatatga attagctaca aaggtttgtg gaccagattt
      601 tctaagtgtt ctagattcta atatagagca aactctagaa aaaaaagatg aaggataagt
      661 aggaggttat ttaagcatcc cctttattta acaaatcatc caaaaaacta gcaacaagtg
      721 acttatctcc aactacatga taactatcta ctactaaaga caattcttta caaaaagaac
      781 tccttttttg cgacaacgca taatcatcta ttgataaaag aggctctgta aaattcttta
      841 ctctcctact acaacctcta atttttctag ttatctcctc atataaatga aattctgttt
      901 tggtatgatt aaatttttca tataacaaag ctcctttggg acaaatttga ggctgcacca
      961 acaaagtaag gggaatccaa ttttttgtca cattaccaaa tctattaatg aaatctgctt
     1021 cgttaatcct gaaatagtcc cctaaatatg gtttgttgaa cttcaatcta ttagtagatt
     1081 ttataaaagg ctgattgttt atgtatctca atagtatttt ctcatcacca agaaaaagat
     1141 acccatgttt taaagccagt tctaaagaaa tacttgtttt tccaatacca cttacttgtc
     1201 ctacaaatac aattgcacgt tggtttttag taactgcact accatgtaaa caatagacac
     1261 ctttactttg tctacaatac tccagtccgt actccagcaa ggtgacaaca tcttttatgg
     1321 aacaatcttc tgagtaacga atcattataa cattatcaat aatatcaaaa gaattacttt
     1381 tcgatacgtt taattctatt ataaaatcag gcttacctcc attaggtaaa ctatcaatcg
     1441 ttaaatctgg aatatactca tataaaaatt gctccttatc gcaaaagagt ttgctcgaat
     1501 tactggttat aacatcaatt ttcaaaccag cattaactag cctcatcaaa aatcttattc
     1561 aatttatcaa catttgactc ccatgtaaat tcggttttag cacgattata tgcattattt
     1621 ccgcaattca atctcagagt actatccgtt agaaacaact ctattttctc agcaagggat
     1681 actttatctc taggttttgc aagcagacca tatttctcat cgataataaa atcaaccata
     1741 ccacagctat ctgtagtaat tgttggacag tattgcgttg atgcctcaag catagtagtt
     1801 agaacccctt ctttagcaaa tgatgggaac acacaaaaac ttgattctct atataattta
     1861 tatttctgtt cttcagaaat atgtccaagg aaaaatatgt tctcgtttaa atcaagtttg
     1921 ttaacaagat tcttcaataa attagtatat tcaatcgttc cagtaccagc tatatacaac
     1981 ttaatgtagg gatatgtatt tatcaacaaa gctattgatt caattaaaac ctccaagcct
     2041 ttaatgtcaa taagcctccc tataaaaaag atttgattgt ccaccctttt aacattttcg
     2101 tatggatata aatctatacc attgttaatg acaataatat ccttgttttt cgagatattt
     2161 aacaatgaat tcttccattc gtttttaata gaattactta cagcaataac attatcagta
     2221 agagtaacgg ctaaatcgtt gtaaacagag tactcagatg gcgtaccatg cagaaataaa
     2281 atctttcttt tgatatgtgg aatacaaaac atacctggag catagtgtat caaacaaaca
     2341 tcaaaattat ccgtaatcgt tttataccaa tcgtacagag ataaatataa taaatgtata
     2401 ttttcgtggg aattaataaa attggcatag ctacatccaa aaattgggct agtaacctct
     2461 acaaatttaa tctgcggatt tcttcttttg aaatcttgat agtaagatgt aggttcttct
     2521 gtcctaactg tgagtattgt aacactatgt ccatttttag acagagcttg cgattgagat
     2581 agtggtgcaa tttcaccacc tcctacgatt atagaagctg ttggtaagaa aaccagaata
     2641 ttttttttca tattattcat ttaatttgtt ctttaatgtt tctaaatttt ttgcaaattc
     2701 attccatttt tcttcctcac tacccgaacg aggaggatga tcaatatgga atatcccagt
     2761 actcaccaca gggatgatca ttgccccaga agcaattaat ttataaccga aatgagtatc
     2821 ttctagcccc caacctgtaa aatcagtatc aaacccatca acagctttat ataaagactt
     2881 actcattgca acattatgcg ttgctacagt cgcagccaag tcaaaaatcc ctataaacct
     2941 attaaatcca aaattcttca acccgtttgt ctccataact atattcctaa aattgctttc
     3001 gttgattttc ctaagtccct ttttagcttc attgatataa caattaaccc tccaatcgca
     3061 ttgaaaatta gatggtttcc tgattccttt gataatattt tgattctcta actcaggatt
     3121 acttggatct aggcactctt taaaagaaat caagacagcg ttaggaatca aacacaatct
     3181 tatgtacatc tcatatacgt agtttgatgg tagcacaaca tcactgtcta agaaaattaa
     3241 tttgttaaat ttagccacag atactcccac atttcgagcg tataccctac caagattctt
     3301 ctctatatta ataatattta tatcaaggtc atcactattc agcattgatt tagtcaattt
     3361 tgattcagag ccatcgttaa caataacaac ctgaattttc tgttttgcta gatgcacaaa
     3421 tgactgacat tgtatactca atactgtctt cttgagactt tcaaacccac cataaacagg
     3481 aattatta
//
LOCUS       DAOPUR010000121         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_27861_length_3452_cov_3.113041, whole genome shotgun sequence.
ACCESSION   DAOPUR010000121 DAOPUR010000000
VERSION     DAOPUR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27861_length_3452_cov_3.113041"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKN90_02975"
     CDS             <1..265
                     /locus_tag="PKN90_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HNZ85274.1"
                     /translation="IKSRLNPSLDIEGFLVTMYDSRLRLANQVYAEVKKHFQDMVFET
                     VIQRNIRLSESPSHGLPVVLYDADSKGAQNHMQLAAEIIKNNS"
     gene            290..1165
                     /locus_tag="PKN90_02980"
     CDS             290..1165
                     /locus_tag="PKN90_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNZ85275.1"
                     /translation="MNKKSGLGRGLGALIEMETVTTSCSSSISEVDITKIQANPDQPR
                     TYFDEEALQELATSIKEVGIIQPITLRQVSSDTYQIIAGERRYRASKIAGLTKIPAYV
                     KTVDDEAVMEMTLVENIQREDLNAIEIALSYQKLIEISGYTQETMSERVGKKRSTIAN
                     YLRLLKLPAEIQLGITNKKIDMGHARALVTISDASVMLQVYEKIVKEGLSVRKVEELV
                     KNLQSLEVEGEQKVTKSTPRLPAEYTALSKRLSDFLHVSVKIERNESGKGKIILPFRS
                     DEELEGLMDVFDKMM"
     gene            1178..1831
                     /locus_tag="PKN90_02985"
     CDS             1178..1831
                     /locus_tag="PKN90_02985"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNZ85276.1"
                     /translation="MKLKYYIFAIFVFSCLSLWASDDSLVVVSKSDSVQEKEVVLYVD
                     STLLHAEFKPQPSKAIWYAAIVPGLGQIYNRKYWKLPILYGGAMGLTYAISWNGGMYR
                     DYQKAYRDIVDNDASTQSYVNILPQGVVIDSDNKAYYTSLLKNKQDNYLRYRDLSIIG
                     TVGLYLLSIVDAYVDAHLYDFDVSPDLSLKIVPKAIPLKHINHFSDVSFGLSCQFSF"
     gene            1854..3263
                     /locus_tag="PKN90_02990"
     CDS             1854..3263
                     /locus_tag="PKN90_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ85277.1"
                     /translation="MFINKIISSLFVIWLVFVTPGYASSIYPSQSSPLLVSEYESSMQ
                     TFDTAIEEMCVAWRMRIKTKETCDSAFRYAPLPYSPDSIRIKRIAMMPTILEMTYNDV
                     VGRYINLYCDRNREISYMLALSQHYFPIFETIFEQYNLPNELKYLSIVESALNPRATS
                     RMGAAGLWQFMPTTGKLYNLEMNSIYDERRDPIKSTHAAAQHFRDLYEIFQDWNLVIA
                     AYNCGTGNVNKAIRRSGGKKDYWEIYPYLPIETRNYVPAFIAVNYMMYYADSYHICPA
                     SIDIPLVTDTLHVTKRIDLLHVSQVLDIPIEQIRLLNPCYKNDIIPDSEKPFILRLPS
                     HLAYLFIEKQDSLYMSQRDVTKQPISLSTSATPHACSSVHIVRSGENLSIIAKRYGIT
                     VRQLMNWNNLTSPLVRVGQRLHLNSSKDISVYVVKKGDSLWTIAKNTSVSVEKIKQAN
                     RNKNLRILQPGQQLTIPKN"
     gene            complement(3315..>3452)
                     /locus_tag="PKN90_02995"
     CDS             complement(3315..>3452)
                     /locus_tag="PKN90_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNZ85278.1"
                     /translation="KRIGLESYGLEIVENIQIVVGINKYNETYMKTKQERMGHNIISS
                     K"
BASE COUNT         1132 a          529 c          654 g         1137 t
ORIGIN      
        1 tatcaaaagt cggctaaatc catcgcttga tattgaaggc tttttagtaa ccatgtacga
       61 ttctcgtttg cggttagcca atcaagtata tgctgaggtt aaaaagcatt tccaagatat
      121 ggtgtttgaa acagttatcc aacgtaacat ccgcttgagt gaatcgccta gtcatggctt
      181 gcctgtagtt ttgtatgatg cagattctaa aggagctcaa aaccatatgc aattggcggc
      241 tgagattatt aaaaataatt cataatatat taagttgtaa tcccgtacaa tgaataaaaa
      301 atcaggttta ggcagaggct tgggtgctct catcgaaatg gaaacggtaa ctactagttg
      361 ttcatcctcg ataagcgagg tggatataac taagatacaa gccaatccag accaaccacg
      421 tacttatttt gatgaagaag ctttgcaaga attagcaaca tcaattaaag aagttggtat
      481 cattcagcca attactttgc gtcaagtgtc tagcgatacg tatcaaatta ttgctggaga
      541 aagacgttat cgtgcttcaa aaatagctgg tttaacaaaa attcctgctt acgttaaaac
      601 cgtagatgac gaggctgtaa tggaaatgac tctggttgaa aatattcagc gtgaagattt
      661 aaatgccata gaaattgcat tgagttatca aaaattgata gagatttctg gttataccca
      721 agaaaccatg agcgagcgtg taggcaaaaa acgttctaca atagctaatt atcttcgttt
      781 gcttaaatta cctgctgaga ttcaactggg tattacaaat aaaaaaattg atatggggca
      841 tgcacgtgca ttggttacaa tttctgatgc aagtgtgatg ttgcaagtat atgaaaaaat
      901 tgtgaaagag ggtctttctg tgcgaaaagt agaagaatta gtaaagaatc ttcaaagtct
      961 tgaagtagaa ggggaacaaa aagttacaaa atcaactcct cgattacctg ctgagtatac
     1021 tgcattgagt aagcgattgt ctgatttttt acatgtttct gttaaaatag aacgtaatga
     1081 aagcggtaaa gggaaaatta ttttaccttt tcggtcggat gaagaattag aaggtttgat
     1141 ggatgttttt gataaaatga tgtaattagt taaacttttg aaactgaaat attacatatt
     1201 tgctatattc gtatttagtt gtctatcttt gtgggcaagt gatgattctt tggttgtagt
     1261 atcaaaatca gactcagtac aagaaaaaga ggtagtgtta tatgttgatt caacactttt
     1321 acacgctgag tttaaacccc aaccttcaaa agctatttgg tatgcagcta tagttcctgg
     1381 gctaggacag atatacaaca gaaaatattg gaaactgcct atattgtatg gaggtgcaat
     1441 gggtttaacg tatgctattt cgtggaatgg aggaatgtat agggattatc aaaaagcata
     1501 tagagatatt gttgataatg atgctagtac ccaaagttat gtaaacatac tacctcaggg
     1561 agtagtgata gatagtgata acaaagcata ctataccagt ttgttgaaaa ataaacagga
     1621 taattattta agatacaggg atttgagtat tattggaacg gtcggtctat atcttttaag
     1681 tatagtagat gcgtatgttg atgctcattt atatgatttt gatgttagtc cagatttatc
     1741 tttaaaaata gtacctaaag ccattccatt gaaacatatc aatcatttta gtgatgtttc
     1801 ttttggactt agttgtcaat tttctttttg ataattaaga ggtagtagaa agaatgttta
     1861 taaataaaat tatatccagt ttgttcgtta tatggttggt gtttgttaca ccaggctatg
     1921 cctcttctat ttatccatcg cagagctctc cgttacttgt ttctgagtat gaatcatcta
     1981 tgcaaacgtt cgatacagca atagaagaga tgtgtgttgc atggcgtatg cgaattaaaa
     2041 caaaagaaac atgtgatagt gcattccgtt acgctccatt accttattcg cctgattcaa
     2101 ttcgtattaa acggattgca atgatgccca caattttgga aatgacgtat aatgatgtag
     2161 taggtcgtta tatcaattta tattgcgaca gaaacagaga aatatcttat atgcttgcac
     2221 ttagtcagca ttatttccca atttttgaaa ctatttttga gcagtacaat cttcccaatg
     2281 aactaaagta tttgtctata gtagaatcag cactgaatcc tagagctact tcgcgtatgg
     2341 gagccgcagg gttatggcaa tttatgccaa ctacaggtaa gttatataac ttagaaatga
     2401 atagtatata tgatgagcga agagacccta tcaaatcaac tcatgcagct gcccagcatt
     2461 ttagagattt atatgaaata tttcaggatt ggaatttagt gatagccgcc tataattgtg
     2521 gaactggaaa tgttaataaa gctatcagaa gatcgggtgg aaaaaaagat tattgggaga
     2581 tttatccata tttacctatt gaaacacgta attatgtgcc tgcatttatt gcagtgaatt
     2641 atatgatgta ttatgcagat agctatcata tttgtccagc tagcattgat attccattgg
     2701 taacagatac tcttcatgtt acaaaaagga tagatttgtt acatgtttct caggtgttgg
     2761 atataccgat tgagcaaata cgtttactta atccctgtta taaaaatgat ataattcctg
     2821 attcagaaaa acctttcatt ttacgacttc cttctcattt agcgtattta tttatagaaa
     2881 agcaagactc tctatatatg tcgcaacgtg atgtgactaa acaaccaatt tctctatcaa
     2941 catcagctac tcctcacgca tgttcatctg tacacattgt acgttctgga gaaaatttgt
     3001 caattattgc caaacgttat ggtataaccg tccgtcaatt aatgaactgg aataatttaa
     3061 cttcaccatt agttcgtgta gggcaacgtt tacatctcaa ttcgtctaaa gatatatctg
     3121 tctatgtggt aaaaaaagga gatagcctat ggactattgc caaaaataca agtgtctcgg
     3181 tagagaagat aaaacaagct aatagaaata aaaatttgcg tattttgcaa ccaggtcagc
     3241 aactgactat tccaaaaaat taaaggaacc aattgcatag tttgattcct ttgtattgtt
     3301 ttagttatgt agggctattt tgacgatatg atattatgtc ccattctttc ttgtttcgtt
     3361 ttcatatagg tttcattgta tttattaata cccaccacaa tttgaatatt ttctacaatc
     3421 tctaatccgt aagattctaa cccaattctt tt
//
LOCUS       DAOPUR010000122         3440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_27992_length_3440_cov_3.295421, whole genome shotgun sequence.
ACCESSION   DAOPUR010000122 DAOPUR010000000
VERSION     DAOPUR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27992_length_3440_cov_3.295421"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /gene="hcp"
                     /locus_tag="PKN90_03000"
                     /gene_synonym="priS"
     CDS             complement(<1..943)
                     /gene="hcp"
                     /locus_tag="PKN90_03000"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNZ85279.1"
                     /translation="MFCNQCQESAHNTGCTIKGVCGKTEDTAGIQDLVVYVCQGLAYV
                     NIEARKQGVNTLPESRKIAQSLFATITNANFDNSLLIKQIKEVIELRNSLHSKLNTPL
                     KHHTVRWNGTTDIEFVAKAALVSVQTYDADIDIRSLKQYVLYGLKGMAAYAEHAANLG
                     QEDGDIYDFIEKALVMITKETSLDDMLSWVMKTGEYGVKVMSLLDAANTNTYGNPEIT
                     KVNIGVGKKPGILVSGHDLKDLEELLEQTKNSGIDIYTHSEMLPSHAYPMFKKYPHLV
                     GNYGNAWHKQLEEFESFNGPILFTTNCLVPPRKSTTYN"
     gene            complement(981..1835)
                     /locus_tag="PKN90_03005"
     CDS             complement(981..1835)
                     /locus_tag="PKN90_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HNZ85280.1"
                     /translation="MLRTIIKIDEELCNGCEACVQGCHEGALQMIDGKARMVSELFCD
                     GLGACIGECPVGAITLEEREAEPYDEIATIERMIPKGENTILAHLKHLKEHGEFDYLK
                     QAVSYLRENGVKVDFAQVHNMPIHGKEGSKGCPGSREVSFANPTPSFRVDTNTTATSA
                     LRQWPVQLHLLNPQAGYFKSADVVLAADCVAFSYANFHQKFLAGKTLAIACPKLDSNK
                     EIYVEKLQSMIEESLINTLTVIIMEVPCCGGLLSLASKAVSQSNRKIPMKLIVVSIKG
                     EILKEEWV"
     gene            complement(1841..2170)
                     /locus_tag="PKN90_03010"
     CDS             complement(1841..2170)
                     /locus_tag="PKN90_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ85281.1"
                     /translation="MEKKEFPKAQVFTFVDSVDYAAGSIVSKILLRNEKGNLTLFAFD
                     KGEFLSEHTAPFDAIVQILDGTAEVIIDKKPHLLTTGQSIIMPANVSHALTATERFKM
                     LLIMIKG"
     gene            complement(2325..>3440)
                     /gene="asnB"
                     /locus_tag="PKN90_03015"
     CDS             complement(2325..>3440)
                     /gene="asnB"
                     /locus_tag="PKN90_03015"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HNZ85282.1"
                     /translation="DCKITPWYKRDWMKYDAVNNNSSSIDELREALEKAVERQLMTDV
                     PYGVLLSGGLDSSIISAVAKKYSTKRIESGSIQDAWWPQLHSFAVGLDGSPDLIAARK
                     VADYIGSVHHEIHFTIEEGLDAVSDVIYHLETYDVTTVRASTPMYLMARVIKSMGVKM
                     VLSGEGADEIFGGYLYFHKAPNAKAFHEETVRKLSKLHSYDCLRANKSLAAWGVEGRV
                     PFLDKEFMDVAMRLNPTDKMAGNGKMEKWILRKAFESYLPEEVVWRQKEQFSDGVGYS
                     WIDNLKLIANERITDTMMKNATHTFPINTPETKEAYLYRTLFTEHFPSRTAAETVPYE
                     RSVACSTAIALEWDAEFKKMADPSGRAVKSVHTDAYK"
BASE COUNT          947 a          681 c          609 g         1203 t
ORIGIN      
        1 tgttgtatgt ggtagatttt cggggaggaa ctaaacaatt ggttgtgaat agtataggac
       61 cattaaacga ttcgaattct tctaattgct tgtgccaagc gtttccataa ttacctacta
      121 agtgaggata ttttttgaac attggatagg catgtgaggg taacatctca gagtgagtgt
      181 aaatgtctat tccgctgttt tttgtttgtt ccaatagctc ttctaaatct tttaaatcat
      241 gtccgcttac caagatgcct ggtttttttc ctacaccaat atttaccttg gttatttctg
      301 gattgccata ggtgtttgta tttgctgcat ctaataatga cattactttt acaccatatt
      361 cgcctgtttt cattacccaa gaaagcatat cgtcgaggct tgtttctttt gtaatcatta
      421 cgagagcttt ttcaataaaa tcataaatat cgccatcctc ttgtcccaaa ttagcggcat
      481 gttcggcata cgctgccata ccttttagtc cgtacaatac atattgtttt agcgaacgta
      541 tatcaatatc tgcatcgtag gtttgtacac ttaccagagc agctttcgca acaaattcaa
      601 tgtcagttgt gccattccac cttacagtat gatgtttgag aggagtgttt aatttactat
      661 gaagcgaatt tcgaagttca attacttctt ttatttgctt tattagtaag ctattatcga
      721 aatttgcgtt tgtaattgtt gcaaacaaac tttgtgctat ttttctactt tcgggtaggg
      781 tatttactcc ttgttttcgt gcctctatgt ttacgtatgc taaaccttga catacatata
      841 ctactaaatc ttggatacca gcagtatcct cagttttacc acatacacct tttatagtac
      901 agccagtgtt gtgtgctgat tcttgacatt gattacaaaa catgctcata ttattatcgt
      961 gttaaatgtt tatttttgag ttatacccat tcttctttta gtatttctcc tttgatgctc
     1021 actacaatta atttcattgg tatttttctg tttgattgcg ataccgcttt gcttgctaaa
     1081 gaaagcaatc caccgcaaca aggaacttcc ataatgataa ctgtaagggt attgattaac
     1141 gattcttcta tcatcgattg tagtttttct acgtatattt ccttattact gtctaatttt
     1201 ggacaggcta tggctaaggt ttttcctgct aaaaattttt gatgaaaatt ggcgtaacta
     1261 aatgcaacgc aatcagcagc cagcactacg tcagctgatt taaaatatcc agcttgtggg
     1321 tttagaaggt gcaattgcac gggccattgc cttagtgccg aagtggcagt ggtgttggtg
     1381 tcaacacgaa aacttggcgt tgggttggca aaagatactt ctcgactgcc tggacaccct
     1441 ttgcttcctt ctttgccatg tatgggcatg ttgtgtactt gtgcaaagtc tacttttaca
     1501 ccattttcgc gtaaatagga aacagcttgt tttaagtagt caaattcccc atgctctttt
     1561 agatgcttta agtgagctag aatagtgttt tcgccttttg gaatcatacg ttcaatggtt
     1621 gcaatttcat cataaggttc agcttctctt tcttctagtg taatggcacc tacaggacat
     1681 tcgccaatac aagctcctag cccgtcgcaa aataattcgc ttaccatacg tgcttttccg
     1741 tcaatcattt gcaaggcacc ttcatggcaa ccttgcacac aggcttcgca tccgttacac
     1801 aattcctcgt caattttaat aattgttctt aacatagtgc ttatcctttt atcatgatga
     1861 gtaacatttt aaaacgttcg gtagcagtta aggcatggct cacatttgca ggcattataa
     1921 tactttgtcc tgttgttagt aaatgtggct ttttatcaat aataacttca gctgttccat
     1981 caagtatttg tacaatggca tcaaatggtg cagtgtgctc acttaaaaac tcgcctttat
     2041 caaaagcaaa tagggtaagg tttccttttt cgttgcgtag aagtatttta ctaacaatag
     2101 agcctgctgc ataatcaaca ctatctacaa atgtgaacac ttgtgctttt ggaaattctt
     2161 tcttttccat actatcatct ttttattttg tagcacaaag atagggtgta tggcacagat
     2221 aaagtgtaac atttgttaca cttacaaaat aaatggttgt gcctaaatct agacacaacc
     2281 atttagaaaa taaaaactat aaaaatgaaa aactaacaga tattttattt gtaggcatca
     2341 gtatgaactg attttacagc tcttcctgat gggtctgcca ttttcttaaa ttcagcatcc
     2401 cactccaatg ctatagcggt actacaggct acagaccgtt cgtatggaac agtttcggct
     2461 gcagtacggc ttggaaaatg ttctgtaaat agtgtgcgat ataaatatgc ctcttttgtt
     2521 tcgggtgtat taattggaaa agtatgagta gcatttttca tcatggtatc ggttatgcgt
     2581 tcattggcaa tcagttttaa gttgtctatc cagctgtatc ctacaccatc agaaaattgt
     2641 tctttttgac gccacacaac ctcttcgggt aaatacgatt caaaggcttt acgcaatatc
     2701 catttttcca tttttccatt accagccatt ttatctgtag gatttaagcg catagcgaca
     2761 tccataaatt ctttgtctaa aaatggaacg cgaccttcta caccccaagc tgctaaggat
     2821 ttattcgctc tcaaacagtc gtaagaatgc agtttggata attttcgcac tgtttcttca
     2881 tgaaaagctt tagcgtttgg agctttatgg aaatacaaat atccaccaaa aatttcgtca
     2941 gcaccctcgc cagataatac catttttact cccattgatt tgattacacg tgccataaga
     3001 tacatagggg tagaagccct aaccgtggtt acatcatatg tttcgagatg ataaataacg
     3061 tcacttacag catctaaacc ttcttcgatg gtaaagtgta tttcgtggtg tactgaacca
     3121 atgtagtcag ctactttacg agcagcaata aggtcgggcg aaccatctaa tcctacggca
     3181 aatgaatgta gttgaggcca ccaagcatct tgaatacttc cagactcaat acgtttggtt
     3241 gaatattttt ttgctactgc tgaaataata gatgaatcca atcctccaga caataatacg
     3301 ccatatggta catctgtcat taattgtctt tcaactgctt tttctagagc ctctcgcaat
     3361 tcatcaatgg aagaagagtt gttgtttacc gcatcatatt tcatccaatc ccgtttgtac
     3421 catggcgtga ttttgcaatc
//
LOCUS       DAOPUR010000123         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_28013_length_3438_cov_3.869938, whole genome shotgun sequence.
ACCESSION   DAOPUR010000123 DAOPUR010000000
VERSION     DAOPUR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28013_length_3438_cov_3.869938"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..1999)
                     /locus_tag="PKN90_03020"
     CDS             complement(332..1999)
                     /locus_tag="PKN90_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ85283.1"
                     /translation="MIKENFIKLFETSFKENWELPAYTDYTLGTSMTYGEVALKIEKL
                     HLLFAESKIKPGDKIALIGKNTPHWAITFVATVTYGAVIVPILQDFNPNDVHHIVNHS
                     ETVFLFCSDSIWDNLEEDKMENIQAAFSIDDFRCIHQKDGAGIQKIVGNLTSKFEKKY
                     PQGFTQSHIQYTSRDNSELAMLNYTSGTTGFSKGVMLTANNLAGNITFGFKTQLLKKG
                     DRVLSFLPLAHAYGCAFDFLVPTCSGSHIYFLNKIPSPKVLLKAFEEIKPNVIFMVPL
                     ILEKVYKKSIQPSLNKTGIKLALNIPLLNKKIYEQVNKKLTESFGGEFTEIVVGGAAL
                     NKEVEEFLTKIGFRFTIGYGMTECAPLISYASHTEFRPSSAGKILDTMQVKIDSPDPY
                     NIVGEILVKGEHVMQGYYKNELATSEAFTDDGWLKTGDLGTIDEHGYIFIKGRSKSMI
                     LGANGQNIYPEEIEAKLNNLPFIMESLVIEKHGKLIALVYPDYEAMDGSQLTQQDLEI
                     IMEENRNNLNNVVGTYENVSKIHLYPNEFEKTPKRSIKRYLYTNISI"
     gene            complement(2096..2875)
                     /gene="mazG"
                     /locus_tag="PKN90_03025"
     CDS             complement(2096..2875)
                     /gene="mazG"
                     /locus_tag="PKN90_03025"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNZ85284.1"
                     /translation="MKKKLEAVENVIHILDELRVKCPWDKKQTNETLRVLTIEETYEL
                     SDAILQGNNQQIKKELGDLLLHILFYAKIGEEKSAFTLKDICDALAEKLIYRHPHIFG
                     TEQAQTSQEVEKNWEKLKLKEKEGNKTILSGVPSSLPSIIKAHRIQDKARSAGFDWEK
                     KEDVWKKVQEEISELTVEIDSMNKEKMEKEFGDVFFSLINAARLYKINPDNALEHTNK
                     KFIQRFTYLEEYAKQHEIALSDMPLAKMEEIWQLAKKEENM"
BASE COUNT         1088 a          536 c          492 g         1322 t
ORIGIN      
        1 caacaacttc aacagctgaa tcaacaacag ctgcagctga atcaacaaca acttcttcta
       61 caacttcttc ttctactaca gcttcttcag tagctttttc tgcacatgaa gcaaaagcaa
      121 cagcaacgat agtagcaaca aataatacta actttttcat tttttttagt ttttaaaata
      181 ttaaacaatg attttttttg aacgcacaaa gatatccatt tattttgtaa caaaaataaa
      241 aaaaataaaa aaacttttgt taataagtgt taataatatg ttagagagca aatatagtat
      301 gcataacggt gtaaataaat gataaaactc cttaaatact gatatttgta tacaaatagc
      361 gtttaatgct tcttttgggt gttttctcaa attcattagg atataggtgt atttttgaca
      421 cattttcgta tgtacctact acgttattta gattgttcct attttcttcc ataataattt
      481 ctaaatcttg ttgtgtaagt tgtgaaccat ccattgcttc gtagtctggg taaactagag
      541 caataagttt accatgtttt tctattacta aactttccat aataaatggt aagttattta
      601 gttttgcttc tatttcttca gggtatatat tttgtccatt agcccctaaa atcatgcttt
      661 ttgatcttcc cttaataaaa atataaccat gttcatcaat agttcctaaa tcgcctgttt
      721 tcaaccatcc atcgtcggtg aatgcttcgg atgtagccag ttcgttttta taatatccct
      781 gcatgacatg ttctcctttt actagtattt ctcctacaat attgtaaggg tcgggtgaat
      841 caatcttaac ttgcattgta tctaatattt taccagccga ggaaggtcta aattcagtat
      901 ggcttgcata tgaaataagt ggagcacatt ctgtcattcc atacccaatg gtaaaacgaa
      961 acccaatttt agttaagaat tcttctactt ctttgtttaa tgcagcacca ccaacaacaa
     1021 tttcagtgaa ttcgccacca aatgattcag taagtttctt atttacttgt tcataaatct
     1081 ttttatttag caatgggata tttagagcta gtttaatacc tgttttattt aacgaaggct
     1141 ggatagattt tttatatact ttttctaaaa taaggggaac cataaaaata acgttaggtt
     1201 ttatttcttc aaatgctttt aacaaaactt ttggacttgg tattttattc aaaaaatata
     1261 tatgagaacc agaacatgtg ggaacaagaa aatcgaaagc acaaccatag gcgtgagcta
     1321 atggtaaaaa agataatact ctatctcctt ttttaagaag ctgggttttg aagccaaaag
     1381 taatattccc tgctagatta ttggctgtta acataactcc tttactaaat cctgtagtgc
     1441 ctgatgtata atttagcatg gcaagttcac tgttatcgcg tgatgtgtat tgtatatggc
     1501 tttgggtaaa gccttgcgga tatttcttct caaacttgct agttagattg cctactattt
     1561 tttgaattcc tgcaccatct ttttgatgta tacatcgaaa atcgtcaatg gaaaatgctg
     1621 cttgaatatt ttccatttta tcttcttcta aattatccca aatactatca ctacaaaata
     1681 aaaagacagt ttcagagtga ttaacaatat gatgtacgtc attgggatta aaatcttgca
     1741 aaataggtac aattacggca ccataagtta ctgtagctac aaatgtgata gcccaatgtg
     1801 gtgtattttt gccaatcaaa gcaattttat ctccaggctt aattttagat tctgcaaaca
     1861 aaaggtgtaa tttttcaatt ttcagtgcga cttctccata cgtcatgcta gtacccaaag
     1921 tataatctgt gtaggcaggt aactcccaat tttccttaaa actagtttca aagagcttga
     1981 taaaattttc tttaatcata gtattttgat ttatgttgtt ttacaaaaat aaactatttt
     2041 ttgatacata tagtttgtag gctaactttt aagaaatttt gttagtaaaa caatgctaca
     2101 tgttttcttc tttttttgct agttgccata tttcttccat tttggcaaga ggcatatcac
     2161 taagagctat ttcatgttgt tttgcatatt cttctaagta ggtaaaacgc tgaataaatt
     2221 ttttattggt atgttctaag gcattatctg ggttaatttt atacaatctg gcagcattaa
     2281 ttaaactgaa aaatacatca ccaaactctt tttccatttt ttctttgttc attgagtcta
     2341 tttccacggt taattctgaa atctcttctt gcactttttt ccacacatcc tctttttttt
     2401 cccagtcaaa accagcactt cgagctttat cttggatgcg atgagctttt ataatggacg
     2461 gaagagagga tggaacgccc gacagtatcg ttttgtttcc ttctttttct tttaatttta
     2521 atttttccca atttttttct acctcttgag aggtttgagc ttgttctgtc ccaaaaatat
     2581 ggggatgtct gtatataagt ttttcggcaa gtgcatcaca gatatctttt agtgtaaaag
     2641 ctgatttttc ttctccaatt tttgcgtaga ataatatatg cagtaataaa tcgcctaatt
     2701 ctttttttat ttgttggttg ttgccttgaa gtatagcatc tgacaattcg tacgtttctt
     2761 caatagtaag aactcgaagg gtttcgttag tttgtttttt atcccaagga catttaactc
     2821 ttaattcatc taatatatga atgacgtttt caacagcttc taattttttt ttcatactta
     2881 tattcttatt tacgatttct cgatagggca gtacaaaact cgtaaataat atttagttaa
     2941 acaaatttct tctacatcac ttatcgactt ttagtgctta aattcttgca taaatgcacg
     3001 aaacgttaca aatatgaaat tctagggagt ttaaatacgt gataattgta tgatttttaa
     3061 gtatttatgt atgtaatttg ttattaacaa tataaacaaa ataggaaatg gtacataatt
     3121 ttaaaataat ggcatcacgt aagtctattt taacagatta ttgattacat atgtaatata
     3181 acaagatgtt aaagttaatt acatattaat tttaggaaaa catagagtag ggtaattgtt
     3241 tattttcaca ttgaattaca tttgtatttt taatagaatc atactctaga ttaaaataat
     3301 ataataatta tcaatttatt gtatattaga tatttataaa tttattatga agtatttatt
     3361 tatatttaat ggtttttacc tcatagtagt agataaaacg caaaatcaca ataaaaattg
     3421 attattatta tattgttg
//
LOCUS       DAOPUR010000124         3436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_28031_length_3436_cov_3.417036, whole genome shotgun sequence.
ACCESSION   DAOPUR010000124 DAOPUR010000000
VERSION     DAOPUR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28031_length_3436_cov_3.417036"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PKN90_03030"
     CDS             complement(<1..888)
                     /locus_tag="PKN90_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNZ85285.1"
                     /translation="MSNKHHLQPFIQRHFIVPIYQWTLKHRLLYPHVRFWIGLLFIVF
                     FLWFLFALPSPLFNKPYSTIIYDKDGNFLSAKIADDYQWRFPVSDSIPYKFQETIRCF
                     EDEYFYYHPGINPLSLARSFVQMVKHKKVISGGSTISMQVIRLSLNNQNRNLFSKIIE
                     ILLAVRLELSYSKNEIINLYASHAPFGGNVVGLEAAAWRYYNRPASNLSWGETAALAV
                     LPNAPSLVFPGKNQEILLKKRNKLLDKLAERNIISHTTCILAKDEPLPSRPFPLPPLA
                     PHLLNKIIKVGKSQQRITTT"
     gene            complement(952..>3436)
                     /locus_tag="PKN90_03035"
     CDS             complement(952..>3436)
                     /locus_tag="PKN90_03035"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNZ85286.1"
                     /translation="FWFTPTEKKSEFQFEVTPEMSPNVFIHISYIQAHNKGKNDYPIR
                     MYGVQAIEVEDPTTHITPVITMKEELKPLEKFTISIKEKTGKPMTFTVSVVDEGLLDL
                     TRYKTPNPWKIFYNNEALGVRTWDLYQYVAGAFTGKISGLFAVGGDFYLNKVGKENTN
                     RFKPVVLHQSPISIGKGETKTLTFQMPNYIGSVRVMVVAGNNGAYGASEKTVAVKQPL
                     MVLATVPRVISPTETMIIPVSVISTDKTIKNATVSIAVDANFEVIDGVKRNVVFEKEG
                     EKIVEFKVRSKKTIASGTIQISASSGNNKATSETNLQIRMPNPPVSQSTMYVIKPGES
                     WSQKIKPIGIHNTNFGSIELSRFYPINLEKRINYLIQYPHGCLEQITSTAFPQLFLSN
                     LMELSEAQKIKIDENIKVCLRKIKTYQCKNGGLSYWPGHADDANEWATNYAGHFMLEA
                     KSLGYEIPDGLLDSWVSYQTQMANNWQFSANDYTSDMNQAYRLYTLALAKKPALSAMN
                     RMRETSGIDAMSKWRLAAGYVLAGKPEIGQQLINGVHVKSNEKKYYQPTFGSEERDQA
                     MVLETLVLLKNQSYSFSVAKDISSFLSSNEWYSTQSLSYMLLAISKFAGYTNENTKLN
                     CALSINGVSTNIQTKSPIHQTQLSYNKQNSDIQAAVVNRSESPLYIFVNQFGIPPMTQ
                     NEANSQNLTMTVKYYDLQQKEISPNVIKQGTDFYAEVIITHPNVRKSYSEMALEHLFP
                     SGWEIMSSRLDANQSEKFKPDEMTYQDIRDDRVYSYFNLSKGEKKTIRVLLHATYIGT
                     YYLPSVRCEAMYDNSIQANSEGKWIEVIK"
BASE COUNT          987 a          602 c          621 g         1226 t
ORIGIN      
        1 ggtggttgta atgcgttgtt gactttttcc aaccttaatt attttgttta aaagatgcgg
       61 agctaatggt ggcaaaggaa agggacgtga gggtaagggc tcgtctttgg ctaatataca
      121 cgttgtgtgg ctaataatat tgcgttcagc aagcttatca agtagcttat ttcttttttt
      181 gagtaaaatc tcttgatttt tacctggaaa aacaagagat ggagcgttag gcaatacagc
      241 aagagcggcg gtttcgcccc aagagagatt agatgcaggt cggttataat atcgccatgc
      301 tgcggcttct aatcctacca cattgcctcc aaatggtgca tgtgacgcat ataggttaat
      361 tatttcattt ttgctatacg ataactctaa acgcacggct aataggattt caattatttt
      421 tgaaaaaaga tttctatttt gattgtttaa actaagacgt attacttgca tactaatggt
      481 gcttccacca ctaatcactt ttttatgctt aaccatttga acgaaagaac gtgccagtga
      541 taatggatta attcctgggt gataataaaa atactcatct tcaaaacaac gtatggtttc
      601 ttgaaattta tatggaattg agtctgatac tggaaatcgc cattgataat catcagcaat
      661 tttggcagat agaaaatttc catctttatc gtaaataata gtagaatagg gcttattaaa
      721 taatggagat ggtaatgcaa acaaaaacca aaggaaaaaa acgataaaca ataaacctat
      781 ccaaaaacga acatgcggat atagcagtcg gtgctttagt gtccattgat aaataggcac
      841 aataaaatga cgctgaataa acggttgaag atgatgctta ttactcacaa tcggaattgt
      901 taaattatag cttgtgcatg ttgagttttt ctgcacaagc tatggttgaa attacttaat
      961 cacttctatc catttccctt cgctatttgc ttgaatgctg ttgtcataca ttgcttcgca
     1021 tcgcacagag ggcaagtagt atgtgcctat gtatgtagca tgtagtaata ctcgaattgt
     1081 tttcttttct ccttttgata gattaaagta agaatatact ctatcgtctc tgatatcttg
     1141 ataagtcatt tcatcaggct taaacttttc gctttggttg gcatctaatc tagaactcat
     1201 tatttcccag ccagaaggga ataggtgttc aagagccatt tctgaatagg attttcgtac
     1261 atttggatgt gtaatgatta cttcagcata aaaatctgtt ccttgtttta tcacattagg
     1321 acttatttct ttttgttgta agtcataata cttaactgtc atcgtcagat tctgcgaatt
     1381 agcctcattc tgtgtcattg gaggaattcc aaattgatta acaaatatat acaaaggact
     1441 ttctgaacgg ttaacaactg ctgcttgtat gtctgaattt tgcttattat agctcaattg
     1501 tgtttgatga atgggacttt tagtttgaat atttgttgat acaccattga tactcaatgc
     1561 acaatttaat ttcgtgtttt catttgtgta ccctgcaaat ttacttattg caaggagcat
     1621 atacgataga gattgtgtac tgtaccattc atttgaagac aagaaagagg atatgtcttt
     1681 tgctactgaa aaagagtatg attggttttt aagtaaaaca agtgtttcaa gcaccattgc
     1741 ttgatctctt tcttcacttc caaaagttgg ttggtaatat ttcttctcgt tgctttttac
     1801 gtgtactcca ttaattagtt gttgtccaat ttcaggtttt cctgctaaga cgtagccagc
     1861 agctaatcgc cattttgaca tcgcatcaat gccagatgtt tcacgcattc tattcatagc
     1921 actcaatgct ggttttttgg ctaaagctaa ggtgtatagc ctgtatgcct gattcatatc
     1981 cgaagtgtaa tcattggcac taaattgcca attgttagcc atttgtgttt gataactaac
     2041 ccaagagtcg agtaatccat ctggaatttc gtatcctagc gatttggctt ctagcataaa
     2101 atgacctgca taatttgtag cccattcatt ggcatcatca gcatgtccgg gccaatagga
     2161 tagacctccg tttttgcact gatatgtttt aatttttctt aaacatacct ttatattttc
     2221 atcaattttt attttttgtg cttcggacaa ctccattaag ttcgaaagaa ataattgagg
     2281 aaatgctgtt gatgttattt gttccaaaca accatgtgga tactgaatta aataatttat
     2341 tcttttttcc aagtttatgg gataaaacct agaaagttct atagaaccaa aatttgtatt
     2401 gtgtatgcct atgggtttaa ttttttgaga ccaagattcg cctggtttaa ttacgtacat
     2461 cgtactttgc gaaacaggag gatttggcat tctaatttgt agatttgttt cagatgtagc
     2521 tttattattg cccgaagaag cagatatttg tatagtccct gatgcaatag tttttttgga
     2581 tcgtacttta aattctacaa ttttttctcc ctctttttca aatacaacgt ttctttttac
     2641 cccatcaatt acctcaaaat ttgcatcaac tgcaatactc acagtagcat tttttatcgt
     2701 tttatctgtt gaaataacac ttactggaat aatcatcgtt tctgtcggac tgattactct
     2761 tggcacggta gccaatacca ttaatggttg ttttacagca actgttttct ccgaagcacc
     2821 gtatgctcca ttgttaccag ctactaccat cactctcaca gagccaatgt agttgggcat
     2881 ttgaaaggtg agtgtttttg tttctccttt gccaatactt atcggacttt gatgcagtac
     2941 aacgggtttg aatcgattgg tattttcttt cccaactttg tttaaataaa aatcacctcc
     3001 tacagcaaat aatccggaaa tttttccagt aaatgcccca gcaacgtatt ggtataaatc
     3061 ccaagtacga actccaagag cttcgttatt ataaaatatt ttccacggat taggtgtttt
     3121 atatcgtgtt aaatccaata atccttcatc tacaacagac actgtaaacg tcataggttt
     3181 tcctgttttt tctttgatag agatggtgaa tttttctaac ggtttaagct cctctttcat
     3241 cgttatcact ggggtgatat gggttgtagg gtcttcaact tcaatggctt gtactccata
     3301 catccgaatg ggataatcgt ttttgccttt attgtgggct tgaatatacg atatgtgaat
     3361 aaacacattg ggagacattt ctggagttac ttcaaattga aattcagact ttttctctgt
     3421 gggcgtaaac caaaag
//
LOCUS       DAOPUR010000125         3421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_28186_length_3421_cov_3.240642, whole genome shotgun sequence.
ACCESSION   DAOPUR010000125 DAOPUR010000000
VERSION     DAOPUR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28186_length_3421_cov_3.240642"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..320)
                     /locus_tag="PKN90_03040"
     CDS             complement(15..320)
                     /locus_tag="PKN90_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HNZ85287.1"
                     /translation="MTGTKKISYIEQQQRTIKGMIAYYCKKKHQQGQLCEECKTLTAY
                     ALARLSACKYGENKPSCRKCPTHCYAPNKRKMMEERMRVVGPKMVYLRYFLSAYENY"
     gene            560..3265
                     /locus_tag="PKN90_03045"
     CDS             560..3265
                     /locus_tag="PKN90_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNZ85288.1"
                     /translation="MNKIVKRILVSFLVLFILVFATLLTAPYFFKDKILTLAKQEVNA
                     LLNARADFSGIKLSFIRNFPNATVQLQDFSIVGVADFEADTLFYAKDFGMVVNLKSLF
                     ADNGYDVKRIEVDEASVKLRILPDGRANWDIMKEDTLAVEDTSAFAFHFKLQDFTIKH
                     TNFSYIDEEGDMQLYLTNINHHTTGDLTADSSLLVTKTTAETVTFVMEGMEYLSKASA
                     VFDATINANLNDMKFTFSENQSQLNAVPFAFDGWLKSIDNGWAMDITLLASEVDFKSL
                     LSLVPAVYATDFASLQADGKVSLTGFVKGDMVGDYYPAFGLQLAVENAWFKYPDLPKK
                     LDQITIKTAITNPGKTLDETVIDVSRFAFRLGNNPFAASMRITTPMSDPNVDFKANGK
                     LNLSDIQEFYPLEQGITLQGLLDVDMLLAGRMSYYEKNQYDKFTFAGKIDITKMHLVY
                     PSLPQALKINKASALINNRFVELSTLDMQIGRNDIQAQGKLENVVPYVLKNQTLDGQL
                     ALQSRYFNASDFMPQDSVQEETDEEPMSVIVLPKNIHFVCTATFDELVYEKMNFTNAN
                     GKLTIADGELTINNMSVNAFGGKMLMNGVYSTTEPEKPRVDFTLQIQDVFFSEVSKQV
                     ETLSKFAPILGNALGKFSTTFSFNSLLQQDMMPDLASFMGSGKLTTSSVGLENVPALN
                     AIAKSLKRDDIVPMVVKDIAMMFEINNGKVTTKPFKFKVKDVDFTLGGQTGLDQSIAY
                     TGNVRLPDKLNLGKFSNMAIAVGGTFTKPTVKVDLLSTLGAVVEEKKQEIKAQVTEKV
                     DDAKEKAVEDARLRRAKALEQANKEADKLLQEAKVAGERLVAEADKKGKALVAQANNP
                     LTKRAAEVSAREMVKEAQKQADNLNKRAEEEAKRILLKAEQSSTF"
BASE COUNT         1086 a          593 c          700 g         1042 t
ORIGIN      
        1 ctttttttat tgatttaata attttcataa gcagaaagga agtagcgtaa atataccatc
       61 tttggaccta ctacacgcat gcgttcttcc atcattttgc gtttattggg agcataacaa
      121 tgagttgggc atttgcggca actaggtttg ttttcgccgt atttgcaggc acttaaacga
      181 gctaacgcat aagctgttaa tgttttgcat tcctcgcaaa gttgcccttg ttggtgcttc
      241 tttttgcaat aataagctat catgcctttt attgttcgct gttgttgttc aatataggat
      301 attttttttg ttcctgtcat tatcgtaaca atacaaatgg agttagcatt ttacaatttc
      361 atcgctaaga taggtataat tatacaaaat ggcactatga agatgtactt aatttcgtgt
      421 gtgtagggca aattttttct agaatcataa aagaagtaaa aaaaactggg ataaacaggt
      481 ataggcattc ttttttatta tttttgtagt ttaaatttca ttcttctgat ttctataatt
      541 tttttaatgt tgctatacta tgaataaaat tgtaaaaaga atacttgtca gttttttagt
      601 gttgtttatt cttgtttttg ccaccttgct tacggctcct tactttttca aagataagat
      661 tttgacctta gcaaaacaag aagtcaatgc actgttgaac gctcgagccg atttttcggg
      721 tataaaattg agttttattc gcaattttcc aaatgcaacc gttcagttgc aagattttag
      781 tatcgttggt gtagccgatt ttgaagcaga taccttgttt tatgccaaag attttggcat
      841 ggttgtaaac ctgaaaagtt tatttgccga taatggttac gatgtaaaac gcatagaagt
      901 tgacgaagca tccgttaagt tgcgtatttt gcctgatggg agagccaatt gggatataat
      961 gaaagaagat accttggcag tagaagatac ctcagcattt gcttttcatt ttaagttgca
     1021 agattttaca attaaacaca ccaatttttc ctatattgac gaagaaggcg atatgcaatt
     1081 gtatcttact aatatcaacc accatactac gggcgattta actgccgata gttcacttct
     1141 tgtaaccaaa acaactgccg aaacggtaac attcgtgatg gagggtatgg agtatctatc
     1201 caaagctagt gccgttttcg atgctactat caatgccaac ttgaatgata tgaagtttac
     1261 atttagcgaa aatcagtccc aattaaatgc tgttcccttt gcctttgacg gttggctcaa
     1321 aagcattgat aatgggtggg ctatggatat tacgttgttg gcaagtgagg tcgattttaa
     1381 gtctttgtta tcgcttgtac ctgctgtata tgctactgat tttgccagct tgcaagctga
     1441 tggcaaggtg tcgttaactg gttttgtaaa aggcgatatg gtaggtgatt attatcctgc
     1501 atttggtttg cagttagcgg tagaaaatgc gtggtttaag tatcctgatt taccaaaaaa
     1561 attagatcaa ataaccatta aaacagccat taccaatcct ggaaaaacct tggatgaaac
     1621 agtaattgat gtgtcacgtt ttgctttccg tttgggaaac aatccttttg ctgcctctat
     1681 gcgtattact acacctatgt cggacccaaa tgtggatttt aaagccaatg gcaaactgaa
     1741 tttatccgac atacaagagt tctacccatt agaacaaggc attaccttac aaggattatt
     1801 ggatgtagat atgttgctag cgggcagaat gtcgtattac gaaaaaaatc agtacgataa
     1861 atttacgttt gcgggaaaaa tcgatattac gaagatgcat cttgtatacc cgtcgttacc
     1921 acaagcactg aaaataaaca aggctagtgc tctcatcaat aatcgttttg tagagctttc
     1981 tacattagat atgcaaattg gcagaaacga catacaagca caagggaaat tggagaatgt
     2041 agtgccgtat gtgcttaaaa atcaaacttt agacggacaa ttagccttgc aatcaaggta
     2101 ttttaacgct agcgatttta tgccacaaga tagtgtgcaa gaagaaacgg atgaggagcc
     2161 aatgtccgta atcgtattgc ccaaaaatat ccattttgta tgtactgcca catttgatga
     2221 gttggtgtac gaaaaaatga attttaccaa cgcaaatgga aaattgacca ttgccgatgg
     2281 agagttaacc atcaataata tgtctgtaaa tgcgtttgga ggtaaaatgc taatgaacgg
     2341 tgtgtatagc acgaccgaac ccgaaaaacc acgtgttgat tttaccttgc aaattcaaga
     2401 tgtatttttc agcgaggttt caaaacaagt agaaacgctg agtaagtttg ctcctatttt
     2461 gggcaatgcc ttgggtaagt tttctactac tttttcgttt aattcgttgt tgcaacaaga
     2521 tatgatgccc gatttagcca gttttatggg cagtggaaaa ctcaccacct catcagtagg
     2581 attagaaaat gtacctgcct taaatgccat agccaaatcg ctaaaacgtg atgatattgt
     2641 gccaatggta gtaaaagata ttgccatgat gtttgaaatt aataatggga aagtaacaac
     2701 gaaacctttt aagtttaaag taaaagatgt tgattttacc cttggtggac aaaccggatt
     2761 agaccaatcc attgcttaca caggtaatgt gcgtttgccc gacaaactta atcttggcaa
     2821 attttctaac atggcaatag ccgttggggg cacatttacc aagccaacag taaaagtaga
     2881 cttacttagc actttgggag ctgtggtaga agagaaaaaa caagaaataa aagcacaagt
     2941 aaccgaaaaa gtggacgatg ccaaagaaaa agcggtagaa gatgcacgcc tgcgtagagc
     3001 caaagccttg gaacaagcca acaaagaagc ggataaatta ctgcaagaag ccaaagttgc
     3061 tggagagcgt ttggtagccg aagccgacaa aaaaggcaaa gcattggttg ctcaggcaaa
     3121 caatccatta actaaacgag ctgccgaagt atctgctcgc gaaatggtaa aagaagctca
     3181 aaaacaggca gataacttga acaaacgtgc agaggaagaa gccaaacgta tcttgctcaa
     3241 agccgaacaa agtagcacgt tttaagtggg tttttgtgca atagacttgt agctttctga
     3301 gctaagagct aacgtacata ctccgtgttt ctctgtgtac tattcttaca cgaagagcac
     3361 gaagaagaca cgaagaaaca ctgagaaaaa aatatataat cctctgtgcc actccgtgaa
     3421 t
//
LOCUS       DAOPUR010000126         3421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_28187_length_3421_cov_3.054070, whole genome shotgun sequence.
ACCESSION   DAOPUR010000126 DAOPUR010000000
VERSION     DAOPUR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28187_length_3421_cov_3.054070"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1023
                     /locus_tag="PKN90_03050"
     CDS             304..1023
                     /locus_tag="PKN90_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ85289.1"
                     /translation="MIEISNNAYDGVIVNNATLPPNKDEFKKEIVRLIQSLTNKKLLW
                     ISIPIEKSDFIPILTDLDFEFHHCDEKNLILIKKLHPDSVVPTSKNYIVGVGAIVLNK
                     GNLLVIKDKLSVGYKLPGGYIDKNESIKKALKREVYEETGITIEFESIVNIGHFIHGQ
                     FGESNLYIVCTAKALSQKIKIHDSAEIAEAKWIDPVEFLHSDNVTMYNKSVVEAIINN
                     KGVKLLEQKITLRVPNGEVFF"
     gene            complement(1303..1617)
                     /locus_tag="PKN90_03055"
     CDS             complement(1303..1617)
                     /locus_tag="PKN90_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85290.1"
                     /translation="MKLLYIISFLAVLVATILYMFFPQVAPFVFSVGVVGIIVFRLRT
                     LYAGSDFRVKRLYVMQNLATLLYIAVAYFMFIQSDIWVFILLVAALVETMVVLRMPNK
                     RE"
     gene            complement(1628..1924)
                     /locus_tag="PKN90_03060"
     CDS             complement(1628..1924)
                     /locus_tag="PKN90_03060"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNZ85291.1"
                     /translation="MKKIVDFFITYILNKYVIASAVFVFVMFTNEDYNIKTRYANAQK
                     IKKLEAEIRYYQEETKKNKEKLDLLQSDKENLERFAREQFIMKKDSEDVFVVVD"
     gene            2195..>3421
                     /locus_tag="PKN90_03065"
     CDS             2195..>3421
                     /locus_tag="PKN90_03065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNZ85292.1"
                     /translation="MDNYIVSARKYRPITFDSVIGQHALVTTLKNAIKNKQLAHAYLF
                     CGPRGVGKTTTARIFAKTINCLQPLPNHEACNQCESCMAFNEQRSFNIHESDAASNNS
                     VDDIRSLIDQVRIPPQIGKYSVYIIDEVHMLSQSAFNAFLKTLEEPPAHAIFILATTE
                     KHKILPTILSRCQIYDFNRISVQDTVTHLQHIASLEGITAEPEALHVIAHKSDGGMRD
                     ALSTFDQVVSFCGNTITYKHVIENLNILDYDYYFKLVDAFLSGNVPASLLLFNEILNK
                     GFDAHNLIVGLSEHLRDVLVCKDERTAILLEVGASIRESYIQQSKACTDAFLYEALSL
                     SNACDLEYKQSKNKRLLIELCLIKLCQILTPKVAQHQGSTPLQVIQTNTTQPQATPIQ
                     KEASKLVATPTYTTPLT"
BASE COUNT         1221 a          616 c          530 g         1054 t
ORIGIN      
        1 caaatagtta gctaaaagcg tttctgagat attttaaagc aagcaagaac ttttatctgt
       61 ataaaaatgt atatctttgc caaagtacct cataattttt cctctatttt ttggggataa
      121 aacagtgttg taaagaactg gaaatgggtt aattagaacg tatggcaaaa atccaaacag
      181 taggaaacga tacacaagat acaggtcagt attcttttta tgttcgcttt caactatagt
      241 agaaagaatt gattctatga atgtagatag cattagcaat catatcgaaa aaaataatca
      301 tctatgatag aaataagtaa caatgcatat gatggagtaa tagttaacaa tgcaacactt
      361 ccaccaaaca aagatgaatt taaaaaagaa atagtacgac taattcaatc tttaacaaat
      421 aaaaaacttc tgtggattag tatcccaatt gagaaatcgg attttatccc aatacttaca
      481 gatttagatt ttgaatttca tcattgcgat gagaaaaatt tgatacttat caaaaaatta
      541 catcctgact ctgttgtccc tacatctaaa aactacatcg taggtgttgg ggctattgtg
      601 ttgaataaag ggaatttatt agtgattaaa gataaattat cggttggata taaacttcca
      661 ggtggctata ttgacaaaaa tgaatcaata aaaaaggctc taaaaagaga agtttacgaa
      721 gaaacaggaa ttactattga atttgaatca attgttaata taggacattt tatacacgga
      781 caatttggag aatctaatct ttatattgtg tgtactgcaa aggctttatc ccaaaaaatt
      841 aagatacacg attcggctga aatagccgaa gcaaaatgga ttgaccctgt agaattttta
      901 cattccgaca acgttactat gtataacaaa agcgttgttg aagctatcat caataacaaa
      961 ggagtcaagc ttttagaaca aaaaattaca ttaagagtac ctaatggaga agtgtttttt
     1021 taaaaagaat ctgattgaca acgcaagcat cgggttaatt gccataaatc tgtacgtttt
     1081 gcaaaacatc gataaaacaa aaaaaatccc ttaacgaaac cttgtggtaa gtactcccaa
     1141 caattacact atataaaatt tacttatatc gttatgctgt aacgcgtata catgttaact
     1201 ttcccgtact ccactgtttt gtaacaatct attgtaaaaa aaggggtagt ttttattgca
     1261 cactttttac taaatcaatg catacgcaaa ctgaccctgc tcttactccc tcttattggg
     1321 catacgcaat acaaccatcg tttctaccaa ggctgctacg agcaatatga atacccaaat
     1381 atcactttga ataaacataa aatacgcaac cgctatatat agtaaggttg ctaaattttg
     1441 cattacatat aatcgtttta ctctaaaatc ggatccagca tagagcgttc gcaaacgaaa
     1501 aacgataata cctacaactc ctaccgaaaa aacaaatgga gctacttgtg gaaaaaacat
     1561 atacaaaata gtagctacaa gtactgctaa aaaagaaata atatacaaca acttcatact
     1621 acaaatttta atctaccacc acaaaaacat cttcactatc tttcttcatt ataaattgct
     1681 cacgggcaaa tcgttccaaa ttttctttat ctgactgcaa taaatctagt ttttctttat
     1741 ttttctttgt ttcttcttgg taatagcgta tttctgcctc tagttttttg attttttgtg
     1801 cgttcgcata ccttgttttg atattataat cttcgttggt aaacatcaca aaaacaaaaa
     1861 cagcagatgc tataacgtat ttgttaagaa tatatgtgat aaaaaaatct actatttttt
     1921 tcatactaga ttgttaaatg gtaactatat taaataaaat tgagatacat atatgcttct
     1981 ttatctgttc ccactaatgg gctactttca tttatccctg caaacaaagg attcctctaa
     2041 aagaacccaa tcacaacaag acaataggta aaccaattat agcagactga agttcaatat
     2101 caacctacaa atgtagtttt ttttctttaa aaagctaaat tttacgttct atttatgaac
     2161 atttttttat ctttgtgcaa aggaaaaaaa gcaaatggat aattacattg tttcggcacg
     2221 aaaataccgc cccataacat tcgattcagt tattggtcag catgcattgg ttactacact
     2281 caaaaatgct attaaaaaca agcaattggc tcatgcttat ttgttttgtg gacctagagg
     2341 tgtaggaaaa acaacaacag ctcgaatttt tgccaaaacg attaactgct tgcaacccct
     2401 acccaaccac gaggcttgca accaatgcga atcgtgtatg gcatttaacg aacaacgctc
     2461 gtttaatatc cacgaatcgg atgctgcctc taataactct gtagatgata ttcggtcatt
     2521 gatagaccaa gtacgtatac caccacaaat tggcaaatac agtgtatata tcatcgacga
     2581 ggtacacatg ctttcgcaaa gtgcgttcaa cgcttttctg aaaaccttag aagaacctcc
     2641 tgctcatgcc atatttatct tggctacgac agaaaaacac aaaattttac caacgatttt
     2701 atctcgttgt cagatttatg attttaatcg cattagcgta caagataccg taactcattt
     2761 gcaacacata gcctctttag aagggattac tgccgagcct gaagctttac acgttattgc
     2821 acacaaatcg gatggtggta tgcgtgatgc actttctact tttgatcaag ttgtcagttt
     2881 ttgtggaaac acaattacat acaaacacgt gattgaaaac ctgaatatat tggattacga
     2941 ctactatttt aaattggtag atgctttttt atcaggaaat gtaccggcaa gtttgctctt
     3001 atttaatgaa attttgaaca aaggttttga tgcacacaat ttaattgtag gattatcgga
     3061 acatttgcgt gatgtacttg tatgcaaaga tgagcgtaca gccatattgt tagaagtagg
     3121 tgccagcatt agggaatcgt atatacaaca atcaaaagca tgcacagatg cttttttata
     3181 cgaagcttta tcgttgagca atgcctgtga tttagagtac aaacaaagca aaaataaacg
     3241 tttattaata gaattatgtc ttattaaatt gtgtcagata ctcacaccca aagtagcaca
     3301 acaccagggt agcacaccat tacaagttat tcaaacaaac acaacgcaac cgcaagcgac
     3361 tcccatacaa aaggaagcta gcaagctggt tgccacaccc acttatacca cacctcttac
     3421 c
//
LOCUS       DAOPUR010000127         3411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_28289_length_3411_cov_3.081347, whole genome shotgun sequence.
ACCESSION   DAOPUR010000127 DAOPUR010000000
VERSION     DAOPUR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28289_length_3411_cov_3.081347"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2089
                     /locus_tag="PKN90_03070"
     CDS             <1..2089
                     /locus_tag="PKN90_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ85293.1"
                     /translation="ARPSGLKEGITYNSNDHTKLHLCLYAKDKNNVLPDNIFVIGDFN
                     NWTMSNSYQMKKDGTTGYWWTELTGLQAGKEYAFQYAVKIGSKIVKISDPYSEKVLHS
                     HDQWEPTQQYPDLMPYPSQGDGYVTVIQTNKPTFQWSTQTLNFQKPNRNNLVIYELWV
                     YDFSPIRNIRAVTARLDYLQNLGVNAIQVMPVAEFEGNMSWGYNPTHYFAMDKAYGTS
                     NDLKRFVDECHKRGIAVILDMVFNHVTGAAPQAKLYWGTSSIATNNPWFNQSAPHGAS
                     VNQDYNHNFGPTRDMFKRVLKYWIDEYKVDGYRMDISHGFCGSDCSNRTDIMFDYYNN
                     GVKAGNEQAYFILEHWEFGDGERENYNQWGMNCHLNNTNSYMQTAMGWLKDGDGLDAS
                     NQDGFISYCESHDEERTMFKAKSYGNGATLRTLAGYCNRVPLNTAFNVLLNGSHMMWM
                     FQELGYDYSIFSNAQGETGDRVDPKPIPESLNWYTNTTRMNAYQKVGQLIQLRTRILP
                     HVFAGDPTNSDLGSGKAARSVIWGSGNDRVFVVGNFNAPSDGETHTGSVNINLPSGNS
                     WYDYLANGSTAMNAGTSVTLQPGDLKIYTAARQTLPTVPNSYSSFPTGGIDIVRDNTV
                     ECNVYPTIATDFISIDSAEDIQDVYVVNMKGYTYPRTLMKNNIRVSDLEQGQYLLIVT
                     FAKEQQAYRFVKK"
     gene            complement(2146..2775)
                     /locus_tag="PKN90_03075"
     CDS             complement(2146..2775)
                     /locus_tag="PKN90_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNZ85294.1"
                     /translation="MGKNKFYVVWRGRETGVFATWELCKRQVHGFEGAQYKSFDTEKE
                     AQLAFQQPFITQIIRKKIVAKPINAKQHGGVPLQDSLAVDAACSGNPGDMEYRGVYVQ
                     TGQEVFRKGVYSNATNNIGEFLALVHGLAFLKQHNSSIPIYSDSVNAIKWVEKKQCKT
                     KLVKTKNNEEVFELIARAEKWLQENTYITPILKWETDVWGEIPADFGRK"
     gene            2932..>3411
                     /locus_tag="PKN90_03080"
     CDS             2932..>3411
                     /locus_tag="PKN90_03080"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNZ85295.1"
                     /translation="MVKRIILSIAFITFIQFANANEGMWILPSIPDSIAKNMDELGYE
                     LKMRDLYNEKKSSLKDVVVSFSNGYTGVMISSDGMLLTNYTAIMPFIEQLDESSQYKE
                     EGFWASSYTREIPLHHLSVSIVKTTENVTNRILANIPEFETQEKKQHLIDSISQLLIK
                     "
BASE COUNT         1149 a          625 c          603 g         1034 t
ORIGIN      
        1 agcacgtcca agtggactga aagaaggtat tacttacaat agcaatgacc atacaaaact
       61 acacctatgt ctgtatgcta aagacaaaaa caacgtatta ccagataata tctttgtaat
      121 aggagatttc aacaactgga caatgtctaa ctcatatcaa atgaaaaaag atggtactac
      181 tggttattgg tggacagaac ttactggttt gcaagctggt aaagaatatg cttttcaata
      241 tgcagtaaaa attggttcaa aaatagtaaa aattagtgac ccttactcag aaaaagtatt
      301 acactcgcac gaccaatggg aaccaacaca acaataccca gacttaatgc cttatccttc
      361 tcaaggagac ggttatgtta ctgtaataca aacaaataaa ccaacttttc aatggagtac
      421 acaaacctta aactttcaaa aacccaatag aaacaattta gtaatttatg agttatgggt
      481 atatgatttt tctccaattc gtaatattag agcggtaact gcccgtttag attatctaca
      541 aaatttgggg gttaatgcca tccaagtaat gccagtagct gagtttgaag gcaatatgag
      601 ttggggatac aaccctacac attattttgc catggataaa gcctatggta cttctaacga
      661 cttaaaacgc tttgttgatg aatgtcacaa acgaggtatt gccgttattt tggatatggt
      721 attcaatcat gttacaggag ctgcacccca agccaaatta tactggggaa caagctcaat
      781 tgctaccaac aatccatggt ttaaccaatc ggcacctcac ggagctagtg taaaccaaga
      841 ttacaatcat aactttggac caacacgaga tatgtttaaa cgtgtattga aatattggat
      901 tgacgaatac aaagttgatg gttatcgtat ggatatttcg catggttttt gtggctctga
      961 ttgtagcaac cgtacagata ttatgtttga ttattacaac aatggtgtga aagcaggaaa
     1021 cgaacaagct tattttattc ttgaacattg ggaatttgga gatggagaac gcgaaaacta
     1081 caaccaatgg ggcatgaatt gccatctaaa caatacgaat agttatatgc aaacagctat
     1141 gggttggttg aaagatggag atggactaga tgcatcaaac caagacggat ttatttctta
     1201 ttgcgagagc catgacgaag aacgcactat gtttaaagca aaatcgtatg gtaatggtgc
     1261 cactttgcgt actttagcgg gctattgcaa tcgtgtaccc ttaaatacag catttaatgt
     1321 actattaaat ggttcgcata tgatgtggat gttccaagaa ttaggctatg attatagtat
     1381 atttagcaat gctcaaggag agacaggaga tcgtgttgac cctaaaccaa tacccgaaag
     1441 tttaaattgg tatacgaaca ctacccgtat gaatgcatac caaaaagtag gacaattaat
     1501 tcaactacgt acgcgtattt taccacacgt atttgctggc gaccctacta actccgattt
     1561 aggaagtggc aaggctgctc gtagtgtcat ttggggatct ggcaatgacc gtgtatttgt
     1621 ggtaggaaac tttaatgctc ctagcgatgg tgaaacgcat actggatcgg ttaacatcaa
     1681 tttaccttct ggaaatagct ggtatgacta cctagccaat ggttctactg ctatgaatgc
     1741 tggtacttct gtaacattac aaccaggaga cttaaaaatt tatactgcag ctcgtcagac
     1801 gttgccaact gtaccaaact catatagtag ttttccaaca ggaggtattg atatcgtacg
     1861 ggataataca gtagaatgca atgtttatcc tacaattgct accgatttta ttagcattga
     1921 ttcagcagaa gatatacaag acgtatatgt tgtaaatatg aaaggataca cctatccacg
     1981 taccttaatg aaaaataata tcagagtatc tgaccttgaa caaggacaat acttgctaat
     2041 tgtaacgttt gctaaagaac aacaagcata ccgttttgta aaaaaataaa caaagagaaa
     2101 tactctaaac ctaaaagaag tgccttgaaa atcaaggcac ttcttttatt ttcgtccaaa
     2161 atcagcagga atttcacccc acacatccgt ttcccacttc aggattgggg taatataggt
     2221 attttcttga agccattttt ctgcccgtgc tattaactcg aagacttctt cgttgttttt
     2281 ggttttaact aatttagttt tacattgttt cttttccacc catttaatag catttacact
     2341 atcagaataa ataggaatag aactattgtg ctgttttaga aaagctaatc catgtactaa
     2401 agccagaaac tcgccaatat tattggtcgc attactgtaa acaccttttc taaacacttc
     2461 ttgacctgtc tgcacataca cgccacgata ctccatatcg cctggattac cactgcatgc
     2521 tgcatctaca gccaaactat cttgcagagg gacacctcca tgttgtttgg cattaattgg
     2581 tttagcaact attttttttc ttataatctg tgtaataaaa ggttgttgaa atgctaattg
     2641 agcttctttt tctgtatcaa atgatttgta ctgagctccc tcaaaaccat gtacctgccg
     2701 tttacacaat tcccaagtag caaacacccc agtttctcta cctctccata ctacgtaaaa
     2761 tttatttttt cccatatgtg cgatatactc ctgcaaaaat acatttttta gatgaatatg
     2821 caatgcgtgt gtagtgtatt agattgagct atacgataac ttattttatc tatactaaat
     2881 taattttgta tatttgcagg taattagcaa ccttatagca ataaaaataa tatggtaaaa
     2941 agaataatct taagtatagc ttttattaca ttcatacagt ttgccaatgc caacgaaggt
     3001 atgtggatat taccatccat accagactcc attgctaaga acatggatga actgggatat
     3061 gaattaaaaa tgcgtgattt atataatgaa aaaaaatctt ctttaaaaga tgtagtcgtt
     3121 tcattttcaa atggatacac aggcgtaatg atttcttcgg acggaatgtt gcttactaat
     3181 tacacagcga ttatgccatt tattgagcag ttagatgaat cctctcaata caaagaagaa
     3241 ggtttttggg caagctctta tacacgcgaa attccattgc atcatctatc tgttagcata
     3301 gtaaaaacaa cagaaaacgt taccaataga attcttgcca acattccaga atttgaaaca
     3361 caagaaaaaa aacaacactt gatagattct ataagtcaac tattaataaa a
//
LOCUS       DAOPUR010000128         3409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_28312_length_3409_cov_3.470185, whole genome shotgun sequence.
ACCESSION   DAOPUR010000128 DAOPUR010000000
VERSION     DAOPUR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28312_length_3409_cov_3.470185"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3409)
                     /gene="purL"
                     /locus_tag="PKN90_03085"
                     /gene_synonym="purI"
     CDS             complement(<1..>3409)
                     /gene="purL"
                     /locus_tag="PKN90_03085"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNZ85296.1"
                     /translation="DNNKLVWLFGNANILDNSSITGNFVGPRREMITPWSTNAVEITQ
                     NMGIEGIVRIEEFYPAGTTGDFDPMLQRKYDKLTQEIFDVIKQPEPIVFIDDIAAYNQ
                     QEGLALNDDEINYLNNISQTIGRKLTDSEVFGFSQVNSEHCRHKIFNGKFVIDGEEKE
                     LSLFKLIKKTSEYNPNKLVSAYKDNVAFNEGPVIEQFAPKSGDKPDSFTTKEIKSVLS
                     LKAETHNFPTTVEPFNGAATGTGGEIRDRLGGGKASLPIAGTAVYMTSYPRIESNRSW
                     EENIKERNWLYQTPEQILIKASNGASDFGNKFGQPLICGSVLTFEHEENNKKYGFDKV
                     IMLAGGVGFGTMRDALKGEAKVGDKVVVMGGDNYRIGMGGGAVSSVDTGQYDNAIELN
                     AVQRANPEMQKRAANVIRTLAESDRNPIVSIHDHGAGGHLNCLSELVESSGGKIDMSK
                     LPIGDSTLSAKEIIGNESQERMGMLMDEKDIDLVQKIADRERAPMYVVGETTGDMQFV
                     FEQANGEKPIDLRLDYMIGNPPRMVITDNTLNETFEEVSVSDSKIEEYITNVLRIEAV
                     ACKDWLTNKVDRSVTGKVATQQCAGELQLPLNNLGAVALDYQGKAGIATSIGHAPLAA
                     LVNPEAGSVLAIAEALTNLVWAPLTEGLTSISLSANWMWPCKNSGEDARLYKAVEACS
                     DFACSLGINIPTGKDSMSMTQKYGNDKVFSPGTVIISAAAEVSDVTKTISPVLVNDQK
                     TALYYVDFSFDALKLGGSVLYQTLNKLGNDTPTVQDSEYFATAFTAVQELIANKLVLA
                     GHDISEGGMITALLEMCFANTKGGLSVNLSELKDFSLTTVLFAENPGVLLQVKNTVEV
                     EKLLTENGIGFAKIATPINDRKLILTKDSTHQELSIDTLRDVWYKTSYLLDRKQSGEV
                     CALQRFENYKNSPLQFAFGTEFTGKLAKYGISNDSRGKSGAKAAIIREKGTNGEREMA
                     YALYLAGFDVRDVHMTDLANGRETLEDIQMIVFCGGFSNSDVLGSAKGWAGGFLYNEK
                     AKTALENFYKRPNTLSLGICNGCQLMIELNLINPEHTKRSRMLHNDSHKFESNFVGLH
                     IPENNSVLFSSLSGSKLGIWVAHGEGKFSLPESEDKYHVIAKYGYNTY"
BASE COUNT          917 a          718 c          626 g         1148 t
ORIGIN      
        1 ataagtgttg tagccgtatt ttgcaattac atggtattta tcttcgcttt cgggcaaaga
       61 gaattttcct tcgccatgtg ctacccaaat gcctagcttg ctacccgaca atgaactgaa
      121 taataccgaa ttgttctcag ggatatgtaa gcctacaaag tttgattcaa atttatgcga
      181 atcgttgtgc aacatacgcg aacgttttgt atgctcagga ttgatgaggt taagctctat
      241 cattaactga cagccattgc aaatacctaa gctcaaagta tttggacgtt tgtagaagtt
      301 ctccagagct gttttagctt tttcgttgta taaaaatccg ccagcccaac cttttgccga
      361 acccaataca tctgagttgg aaaaacctcc gcagaataca atcatttgta tatcttcaag
      421 tgtttctcta ccattggcta agtctgtcat gtgcacatcg cgtacatcaa aaccagctaa
      481 gtatagtgca tacgccattt cacgttctcc gttcgttcct ttttcacgaa taatagctgc
      541 ttttgctccg ctttttccgc ggctgtcgtt gctaatgcca tattttgcta attttccggt
      601 aaattctgtg ccaaaggcaa actgaagtgg actattttta taattttcaa aacgttgaag
      661 ggcacaaacc tctccacttt gttttctgtc aagtaaatac gatgttttat accacacatc
      721 acgaagtgta tcaatcgata attcttggtg cgtgctatct tttgttagta ttaatttacg
      781 gtcattgatg ggtgttgcaa ttttggcaaa tccaatacca ttttctgtta atagtttttc
      841 aacctcaact gtatttttaa cttgtagtaa aacccctggg ttttctgcaa ataaaaccgt
      901 tgtaagcgag aaatctttta attcagatag attgacagat aaaccacctt tggtattagc
      961 aaaacacatt tcaagtaatg cagtgatcat accgccttcg gagatgtcgt gaccagcaag
     1021 caccaactta ttggcaatca actcttgaac agctgtaaaa gctgttgcaa agtattcgct
     1081 gtcttgaacg gtaggtgtat catttcctaa tttgttaagt gtttgataca gtactgagcc
     1141 tccaagtttt aaggcatcga atgaaaaatc aacgtaataa agagctgttt tttggtcatt
     1201 aaccagaaca ggcgaaattg tttttgttac atcgcttact tcagctgctg ccgaaataat
     1261 aaccgtacca ggagaaaaga ccttatcatt gccatatttt tgtgtcatcg acatactgtc
     1321 tttgcctgta gggatattta ttcctaatga gcaagcaaaa tcgctacaag cttctacggc
     1381 tttgtatagt cgagcgtctt ctccgctatt tttgcagggc cacatccaat tggctgataa
     1441 tgaaatactg gttaaacctt ctgttaatgg agcccaaacc aagttagtaa gtgcttcagc
     1501 aatagctaat accgagcctg cttctgggtt aaccaaggca gctaatggag catgaccaat
     1561 agaagttgct atacccgctt ttccttggta atctaaggct acagctccca agttatttaa
     1621 tggtaattgc aattctccag cacattgttg agtggctact tttcctgtta cagagcggtc
     1681 aactttattg gtcaaccaat ctttacacgc aacggcttca atacgcaata cattggtaat
     1741 atattcttca attttgctat cagaaacact aacttcttca aatgtttcgt taagggtatt
     1801 gtctgttatt accatacgag gagggtttcc aatcatgtaa tccaaacgca aatcaatggg
     1861 tttttcgcca tttgcttgtt caaatacaaa ttgcatatcg cccgtagttt cgcctaccac
     1921 gtacatcgga gcacgttctc tgtcagcaat tttttgcact aggtcaatgt ctttttcgtc
     1981 cattagcatc cccatgcgtt cttggctttc gtttccaata atttctttgg cagatagggt
     2041 agagtctcct atgggtaatt tgctcatatc aatttttcca ccactacttt ctactaattc
     2101 tgacaagcag tttaggtgtc cgcctgcacc gtggtcgtga atagaaacaa taggattgcg
     2161 gtcgctttct gctaaggtac ggataacgtt ggctgcacgt ttttgcattt ctgggttggc
     2221 acgttgcaca gcatttagtt caattgcatt gtcgtattga cctgtatcaa cagatgatac
     2281 agcaccacca cccataccaa tgcggtaatt gtcgccgccc atcactacta ctttatcgcc
     2341 aacttttgct tcgcctttta gagcatcgcg catggtacca aaaccaacac cacctgccaa
     2401 catgattact ttatcaaaac catatttttt gttgttttct tcgtgctcga acgttaaaac
     2461 tgaaccacaa ataagcggtt gtccaaattt atttccaaaa tcagatgcac cgttcgatgc
     2521 tttgattaat atttgttcgg gtgtttggta taaccaattg cgttctttta tattttcttc
     2581 ccacgaacga tttgattcga tgcgaggata cgatgtcata tatacagctg ttccagctat
     2641 gggcaaactc gcttttcctc cgcccaagcg gtcgcgaatt tctccacctg tgccagtagc
     2701 agcaccatta aagggttcaa ccgtagttgg aaagttgtgt gtttctgctt ttaacgatag
     2761 tacagattta atttctttgg ttgtgaatga atctggttta tcgccacttt taggggcaaa
     2821 ttgttctatc acaggacctt cgttaaatgc tacattatct ttgtaagcag atactaattt
     2881 gtttgggttg tattcagacg tttttttgat taatttaaac agagaaagtt ctttttcttc
     2941 tccgtcaata acaaatttgc cgttgaaaat tttatggcga cagtgttctg aatttacttg
     3001 cgaaaaacca aaaacttcgc tatcggttag ttttctacct attgtttgac taatgttatt
     3061 gaggtaatta atttcgtcat cgtttaatgc caaaccttct tgttggttgt aagcagcaat
     3121 atcatcaata aatacaatgg gttctggttg tttgatgaca tcaaaaatct cttgagtaag
     3181 tttgtcgtat ttgcgttgca acattgggtc aaaatcgcct gtagtaccag caggataaaa
     3241 ctcttctata cgaacaatgc cctcgatgcc catattttgg gttatttcca ccgcatttgt
     3301 actccatggc gtaatcattt cgcgtcttgg tccaacaaaa tttccagtga tggaactgtt
     3361 atccaatatg ttagcatttc caaataacca aactaacttg ttattgtct
//
LOCUS       DAOPUR010000129         3407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_28334_length_3407_cov_3.782518, whole genome shotgun sequence.
ACCESSION   DAOPUR010000129 DAOPUR010000000
VERSION     DAOPUR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28334_length_3407_cov_3.782518"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKN90_03090"
     CDS             <1..468
                     /locus_tag="PKN90_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HNZ85297.1"
                     /translation="LCIWGERVNVPVIKQKMGADPASVAFDTISSAKANNADVVIIDT
                     AGRLHNKVNLMHELSKIKNVMQKVIPTAPHEVLLVLDGSTGQNAFEQAKQFTKATQIT
                     ALAITKLDGTAKGGVVIGISDQFKIPVKYIGLGEKMEDLQLFDKTAFVQSLLG"
     gene            475..1776
                     /gene="rimO"
                     /locus_tag="PKN90_03095"
     CDS             475..1776
                     /gene="rimO"
                     /locus_tag="PKN90_03095"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNZ85298.1"
                     /translation="MKKNHIDLITLGCSKNLVDTEKLMYQLKVAGYTVAHDAENPTGE
                     IVVINTCGFIGDAKEESIDMILAFAEKKKKRKINKLFVMGCLSQRYLQELAIEIPEVD
                     KFYGKFNYTDLLKELGKEYRFDCSLQRTITTPMHYSYIKISEGCNRTCSYCAIPLITG
                     KHQSRKIEDIEEEVRWLVKQGVKEFQLIAQDLSYYGLDNYKKHKLAELTNRLADIDGV
                     KWLRLHYLYPVDFPMDVLNVMRERDNVCNYLDIALQHISDNMLKKMRRGISSQQTYDL
                     IRQIREQVPGIHIRTTMLLGHPGETDEDVEALKECVRTMRFERLGAFAYSDEEATYSN
                     KHYVDNIPYEIKQQRVDEIMKIQQQISLEHNQAKIGKKFKVIVDSLQGDYYVARSEYD
                     SPEVDMEILLKAEKNHLQIGEFYTATIIGAEEFDLYAEIIK"
     gene            1814..2107
                     /locus_tag="PKN90_03100"
     CDS             1814..2107
                     /locus_tag="PKN90_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ85299.1"
                     /translation="MNNKELISELSKRLRVSQNDISNLLASTVSAFSTAFQQGNDVSL
                     QGFGIFQIRKKNERIIVNPSTNVRQIVPPKLTVIFKMSNTYKEKIKNIIPHGK"
     gene            2097..3107
                     /locus_tag="PKN90_03105"
     CDS             2097..3107
                     /locus_tag="PKN90_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ85300.1"
                     /translation="MENKKQSIQELVDIIATQTNFTKKNSEEFLKEFVHVLQEYLEKD
                     GFVKVKGLGTFKLVKNEERKSVNVTTGEAIIIPAHNKVSFIPDNAIKEAVNKPYAHLE
                     TITVDDDTKNSTIDAQPDNTVREEKSIQDEAKNNSIPNNTVIQHEKPSNNSKKTTKNK
                     KKNPVAIVILLLLFHVAMAVSFYFYKTEIVSFFQRAVTIEENDLPQATIIDKQLSPVS
                     IVADTVVVVETDSMPDTTNIQQPDEIIATYTPELAQNAPIKEYITVNEGSRLTWIAYK
                     AYGHKAFWVYIYDANRNQLKTPNDVKIGMELAIPELDASLVNPNDENCVIKALELAEK
                     YK"
     gene            3249..>3407
                     /locus_tag="PKN90_03110"
     CDS             3249..>3407
                     /locus_tag="PKN90_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNZ85301.1"
                     /translation="MSEIVDIRELNARIEQKSAFVDVLTIGMNKTIVGQKHLIESLLI
                     GLLSDGHIL"
BASE COUNT         1276 a          491 c          638 g         1002 t
ORIGIN      
        1 ttatgcatat ggggagaaag ggtaaatgtg cctgttataa agcaaaaaat gggggcagac
       61 cctgcctctg ttgcttttga tactatatcg tcagctaagg caaataatgc tgatgttgtg
      121 ataattgata ctgctggtcg tttgcataac aaagtgaatt tgatgcacga attatctaaa
      181 attaaaaatg tgatgcaaaa agtaattcca acagcacctc atgaggtatt gttagtgctt
      241 gatggttcta ctggacagaa tgcttttgag caagcaaaac aatttacaaa agctacacag
      301 ataactgctt tggctatcac aaaattagac ggaacagcca aaggcggagt tgttatagga
      361 atttctgatc aatttaagat acctgttaaa tatattggat tgggtgaaaa aatggaagac
      421 ttgcaattgt ttgacaaaac ggcttttgta caatctttgt taggataatt tgcgatgaaa
      481 aaaaatcata ttgatcttat taccttaggg tgttcaaaaa atttagttga tacagaaaaa
      541 ttaatgtatc aactaaaagt ggcaggttat acggtagctc atgatgctga aaatccaact
      601 ggagagattg ttgtaattaa tacttgtgga tttattggag acgccaaaga agaaagtatt
      661 gatatgattc tggcttttgc ggaaaagaaa aaaaaacgga aaataaacaa attattcgta
      721 atgggttgtc tttcacaacg ttatttacaa gaattggcta tagagattcc agaagtagat
      781 aaattttacg gaaaatttaa ttatacagat ttactcaaag aattgggcaa agagtatcgg
      841 ttcgattgta gcttgcaacg taccatcact acgcccatgc attattcata tatcaagata
      901 tcagaaggat gtaatcgcac gtgctcttat tgtgcgatac ctttgataac cggcaaacac
      961 caaagtcgaa aaatagaaga tatcgaagag gaggtacggt ggttagtaaa gcaaggcgta
     1021 aaagaatttc aattaatagc acaagattta tcttattatg gtcttgataa ttacaaaaaa
     1081 cacaaattag ctgaattgac aaatcggtta gctgatatag atggtgtaaa atggttacgt
     1141 ttacattatt tatatcccgt tgattttccg atggatgtgc tgaacgtaat gagggagcga
     1201 gataatgtgt gcaattatct agatattgca ctacagcata tctcagataa tatgttgaaa
     1261 aaaatgagaa gaggaatatc ttctcaacaa acctacgatt tgattcgcca aattcgagaa
     1321 caagtgccag gtattcatat tcgtacaacc atgttattag gtcatcctgg agaaacggac
     1381 gaagatgtag aagctctcaa ggagtgtgtt agaactatgc gttttgagcg gttaggagca
     1441 tttgcttatt cagatgaaga agctacttat tctaacaaac attacgtaga taacattcct
     1501 tatgaaataa aacaacaacg tgttgacgaa ataatgaaaa tccaacaaca gatatctttg
     1561 gagcataatc aagctaaaat tggtaaaaaa ttcaaggtaa tagtggatag tttgcaagga
     1621 gactactatg tggcacgttc tgaatatgat tctccagagg tagatatgga aattttactt
     1681 aaagcagaga aaaaccacct tcaaatagga gaattttata ctgcaacaat cattggggca
     1741 gaagaatttg atttgtatgc tgaaataata aaataaatct gtatatttgt cgttgtctta
     1801 aaactatatt actgtgaata ataaagaatt aatcagcgaa ttgtctaaac gtttacgtgt
     1861 gtcgcaaaat gacataagta atttgttggc atctaccgtt agtgcttttt ctactgcatt
     1921 tcagcaagga aatgatgttt cattacaagg gtttggtata tttcaaataa gaaaaaagaa
     1981 cgaacgaatt attgtcaatc cgtctactaa tgttcgacag attgtgccac caaaattaac
     2041 agtgattttt aaaatgagta acacatacaa agaaaaaatt aaaaacataa tacctcatgg
     2101 aaaataaaaa acaatccata caggaattag ttgatatcat tgccactcaa acaaatttca
     2161 caaaaaagaa ttcagaagaa tttttaaaag aatttgttca tgtattgcaa gaatatcttg
     2221 aaaaagatgg ttttgtaaaa gtgaaaggtt tgggtacttt taaattagta aaaaacgaag
     2281 agcgaaaaag tgtaaatgta acaacgggag aagcaattat catccctgca cataacaaag
     2341 tttcctttat cccagataat gctataaaag aagccgtaaa taagccttat gcgcatcttg
     2401 aaacaattac tgttgatgat gatacaaaaa atagtactat tgatgctcaa cctgataata
     2461 ccgttagaga agaaaagtcc atacaggatg aggcaaaaaa taatagtatt cccaataaca
     2521 ccgtaattca acacgaaaaa ccctcaaata atagcaaaaa aacaacgaag aataaaaaga
     2581 aaaatcctgt tgctatcgtt attttattac tcctttttca tgtagccatg gcagtttcat
     2641 tttattttta caaaactgaa atcgtgtcct tttttcaacg ggcggtaact attgaagaaa
     2701 atgaccttcc tcaagctact attatagaca aacaactttc tccagttagc atagtagctg
     2761 atacggttgt agtagttgaa acagatagta tgccagatac cacaaacata caacaacctg
     2821 atgaaataat agcaacttat acaccagagc ttgctcaaaa tgctccaatc aaggagtata
     2881 ttacagtaaa tgaaggaagt cggttaacat ggatagctta taaagcctat gggcataaag
     2941 ctttttgggt ttatatttat gatgccaatc gtaatcaatt aaaaactccc aacgacgtaa
     3001 aaattgggat ggaattggct atcccagaat tagatgcatc gttggttaat cctaatgacg
     3061 aaaattgtgt tattaaggca cttgaattag ctgaaaaata taagtaataa taagatatag
     3121 aaagaaagtc acgccatgaa tgtgtggctt tttttgttta acaatttatc aaggttttta
     3181 acaaggtttt aaaccaagaa tatcagaaaa tagtgtaatt tcgtatccgt attaaaacaa
     3241 cccaaaatat gagtgaaata gttgatatta gagaattaaa tgcacgtatt gaacagaaaa
     3301 gtgcttttgt ggatgtgtta acaattggaa tgaacaaaac aattgtaggg cagaaacatt
     3361 tgatagaatc attattgata ggtttactgt ctgatggaca tatcttg
//
LOCUS       DAOPUR010000130         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_28468_length_3395_cov_2.710778, whole genome shotgun sequence.
ACCESSION   DAOPUR010000130 DAOPUR010000000
VERSION     DAOPUR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28468_length_3395_cov_2.710778"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKN90_03115"
     CDS             <1..205
                     /locus_tag="PKN90_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNZ85302.1"
                     /translation="ANLIESLGSKNVAEVYVATLLFKPEALEHTVPLDYVAIEIPNDF
                     IVGYGLDYDGYGRNLRAIYTLIP"
     gene            243..815
                     /locus_tag="PKN90_03120"
     CDS             243..815
                     /locus_tag="PKN90_03120"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNZ85303.1"
                     /translation="MLNLVIFGAPGSGKGTQSELIAKKYNLVHLSTGDLLRKEIAEQT
                     ELGKLAKGIIDQGKLVSDDVIINLLESNIAKYPNANGFIFDGFPRTVVQAEELDSMLK
                     KRNIRVSVMVNIDVDSDELVKRLLLRGQISGRTDDNMETIKKRIQTYEDQTIPVIQFY
                     HKQGKHVKIQGIGTIDEIFERVSTAIDAVK"
     gene            898..1998
                     /gene="obgE"
                     /locus_tag="PKN90_03125"
     CDS             898..1998
                     /gene="obgE"
                     /locus_tag="PKN90_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNZ85304.1"
                     /translation="MSNSNFVDYVKIYCRSGKGGAGSMHLHREKFITKGGPDGGDGGR
                     GGHIILRANANYWTLLHLKYARHVFAGHGEKGSGSRSFGKDGEDQIIEVPCGTVAYDA
                     ETGEFVCDVTDHGQEVILLKGGRGGLGNWHFRSATNQTPRYAQPGEPMQEKTIILELK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPKIDNYAFTTLEPNLGIVSYHDHKSFVMADIP
                     GIIEGAHEGRGLGLRFLRHIERNSVLLFMVPADSGDIHKEYRVLLNEIEQYNPELLDK
                     RRVLAISKSDMIDDELELAMKKDLPSIPCVFISSITGKGIAELKDKLWDEIMAEPIKD
                     IVHKSKFVEIADDEVVDDEEPFYEEDSEEDDE"
     gene            2087..2401
                     /locus_tag="PKN90_03130"
     CDS             2087..2401
                     /locus_tag="PKN90_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNZ85305.1"
                     /translation="MNIFVGSLSFNVKDDQLKAAFENFGEVSSARVLTDKFTGRSKGF
                     GFVDMPDNAAAQKAIDALNGTELEGRAMVVNEARERTEDSRRPSTGGYQKRDNSGFKK
                     RY"
     gene            2543..>3395
                     /locus_tag="PKN90_03135"
     CDS             2543..>3395
                     /locus_tag="PKN90_03135"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HNZ85306.1"
                     /translation="MPGTLVNLSSFLQQQLSQWDVAYTNYQALQSVKKRVIEAQTGKL
                     IVQYNPARIVSSAAKTDAKSIEARPCFLCATNRPQEQLQYSYHNTYDILVNPFPIFPE
                     HFTVPHKQHIPQQIRPFFGDMLNLACDFPSYVVFYNGPACGASAPDHLHFQMGNKGFL
                     PIEKEYDTLPKKPVNSFSYLQYQSIETDFYHAIVIESDTKQDVEKRFSFLYKQLQKTS
                     AEPMMNVVCWHTGTNFVVIVLPRTKHRPTQFFATDDTKIVLSPASVDLGGVFITPIEK
                     DFTTLQLA"
BASE COUNT         1138 a          516 c          680 g         1061 t
ORIGIN      
        1 ggctaatttg atagaatctt taggctcgaa aaacgttgct gaagtatatg ttgccacgtt
       61 gctgtttaaa ccagaagctt tggaacatac agtgccatta gattatgtgg ctattgaaat
      121 acccaatgat tttatcgtcg gctatggctt agattacgat ggttacggac gaaatttacg
      181 tgccatttac accttaatac cataaacaaa ctatatataa ttttataaat aagaaaaaga
      241 atatgttaaa tctcgttatt tttggagctc ctggctcagg caaaggtact cagagtgagt
      301 taattgcaaa gaaatataat ttagtacacc tttcaacagg ggatttgtta cgcaaagaaa
      361 ttgctgaaca aacagagctt ggtaaattgg caaaaggtat tattgatcaa ggtaaattag
      421 tgtctgatga tgtaattatt aacctcttag aaagcaatat agctaaatat ccgaatgcaa
      481 atggctttat tttcgatggt tttcctcgca cagtagtaca agcagaagag ttagattcga
      541 tgttgaaaaa acgcaatatt cgtgtaagtg tgatggttaa tattgatgtt gattcagacg
      601 aattggtaaa acgtttgttg ctaagaggtc aaatatctgg acgtacagat gacaatatgg
      661 aaactatcaa aaaacgtatt caaacttacg aagatcaaac aatcccagtt atacagtttt
      721 atcacaaaca aggtaaacat gtgaagattc agggtattgg tactattgac gaaatttttg
      781 aacgtgtgtc tactgctatt gatgcggtaa aataatacat actatgttta tttatacaga
      841 tagtatcgat actatttcgg actatctgtt ttttttcttt ttatacaaaa ccaatctatg
      901 tctaattcaa attttgtaga ttacgtaaaa atctattgtc gttcgggtaa aggtggtgca
      961 ggttcgatgc acttacacag ggaaaaattt attaccaaag gaggacctga tggaggtgat
     1021 ggaggtagag gggggcatat tatattaaga gcgaatgcca attattggac tttgttgcac
     1081 ttaaaatatg ctcgtcacgt atttgctgga cacggcgaaa aaggcagtgg tagtcgaagt
     1141 tttggtaaag atggtgaaga ccaaataatt gaagttcctt gtggcactgt agcttatgat
     1201 gccgaaacgg gagaatttgt atgtgatgta actgaccatg gtcaagaagt cattttgctc
     1261 aaaggaggaa gaggaggttt aggtaattgg cattttcgct cggcaacgaa tcaaactcct
     1321 cgttacgctc aaccaggaga gccaatgcag gaaaaaacca ttattttgga acttaaaatt
     1381 cttgctgatg tagggctagt tggtttcccg aatgcaggaa aatctacctt actttcagta
     1441 gtatctgcag ccaaacccaa aattgataat tatgctttta caactttaga accgaattta
     1501 ggcattgtaa gttatcatga ccataaatcg tttgtcatgg cagatattcc tggtattatt
     1561 gaaggagcac acgagggtag aggtttaggt ttacgatttt tacgccatat cgaacgtaat
     1621 tccgtgttat tatttatggt tccagctgat agtggggata ttcataaaga ataccgtgta
     1681 ttacttaatg aaatagaaca atataatcct gaactattag acaaacgtag ggtattggca
     1741 attagtaagt cagatatgat tgatgatgaa ttagagttgg cgatgaaaaa ggacttacca
     1801 agcattcctt gtgtatttat atcttcaatt actggaaaag gtattgctga attgaaagat
     1861 aaattgtggg atgaaattat ggctgaacca attaaagaca ttgtacacaa atctaaattt
     1921 gttgaaatag ctgatgatga ggtggttgat gatgaagaac ctttctacga agaagatagc
     1981 gaggaagatg atgaataagc ttaaaacaat cttatttttt agataatagt ttgtaaacaa
     2041 gaaaaataca ttatctttgt cgaattattt ttaatacata taacgaatga acatttttgt
     2101 aggaagtcta tcttttaacg taaaagatga ccaattgaaa gcagcctttg agaattttgg
     2161 agaagtatct tcagcacgag ttttaactga taaatttact ggaagaagta aaggttttgg
     2221 atttgttgat atgccggata atgcagcagc acaaaaagct atcgacgcat taaatggtac
     2281 agaactagaa ggtcgtgcaa tggtagtaaa cgaggctaga gaaagaactg aggactctcg
     2341 cagaccaagt actggtggtt accaaaagag agataacagc ggttttaaaa agagatatta
     2401 ataaggtgta aatctttatt atgagaaaaa gagcatttta ttatttaaaa tgctcttttt
     2461 gtttgtaatt agctttgttc aaaggataat ttacaaagaa aaagattgta tttttgtgtt
     2521 ttgatgtaat acaacttgaa acatgccagg tacactagtt aatttatcct cttttttaca
     2581 gcaacaactt tctcagtggg atgtagcata tacgaattac caagccttgc aatcggttaa
     2641 aaaacgtgta attgaggcac aaacaggtaa gttgattgtt cagtataatc ccgcacgtat
     2701 tgtatcatcg gcggctaaaa ctgatgccaa aagtatagaa gcacgccctt gctttttgtg
     2761 tgctaccaat agacctcaag agcaattgca atattcgtat cataatacat acgatatatt
     2821 ggttaaccca tttccaattt tccctgaaca ttttactgtc cctcacaaac aacatattcc
     2881 acagcaaata cgaccatttt ttggcgatat gctcaatttg gcttgtgatt ttccgtcgta
     2941 tgtagttttt tataatggac ccgcatgtgg agcttctgct cccgatcatt tgcattttca
     3001 aatgggtaat aaagggtttt tacccataga aaaagagtat gataccttgc ctaaaaaacc
     3061 agttaactct ttttcttatc ttcaatatca atcaatagaa acagattttt atcatgctat
     3121 tgttatagaa tcggatacca agcaggatgt tgaaaaacgt ttctcttttt tatataaaca
     3181 actacaaaaa acaagtgcag agccaatgat gaatgtagta tgttggcata caggcacaaa
     3241 ttttgtggta atcgtattgc cacgcaccaa acaccgacca acacagtttt ttgctacgga
     3301 tgacacaaaa atagtgctaa gtccagcttc ggtagattta ggtggggtat tcattacacc
     3361 catagaaaaa gattttacta cattgcaatt agctg
//
LOCUS       DAOPUR010000131         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_28741_length_3371_cov_3.020205, whole genome shotgun sequence.
ACCESSION   DAOPUR010000131 DAOPUR010000000
VERSION     DAOPUR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28741_length_3371_cov_3.020205"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKN90_03140"
     CDS             complement(<1..517)
                     /locus_tag="PKN90_03140"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HNZ85307.1"
                     /translation="MQNKGLVLSFTIALVLVCLFYLSFNVVTSHYNKQAKEFAQGNVI
                     EEYRYLDSLSTEKVWLNYTLKECREKELNLGLDLKGGMNVIIEVSVPDILKTLAGNST
                     DETFTKAIQLAHQRQATSQKGFIDLFQEAFQEIDKDARLAAIFSTYELKDKVNLKSTN
                     AEVIAVIKEEVQ"
     gene            complement(651..1700)
                     /gene="queA"
                     /locus_tag="PKN90_03145"
     CDS             complement(651..1700)
                     /gene="queA"
                     /locus_tag="PKN90_03145"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNZ85308.1"
                     /translation="MKLSQFKFKLPEELIATHPTKFRDESRLLVLNKKTGEIEHKVFK
                     NVYEYFSENDVFVFNDTKVFPARMFGNKEKTGARIEVFLLRELNRELKLWDVLVDPAR
                     KIRIGNKLYFGDNDELVAEVIDNTTSRGRTLRFLYDGNYETFKQLLYSLGETPLPRSL
                     NREAEPEDAERYQTIYAKNEGAVVAPTAGLHFSRELLKRMEIRGIHFANVTLHAGLGN
                     FRDIDVEDLTKHKMDSEQMMVTNEACQIVNAAKENRQQVCAVGTTVLRSLETAVSTNG
                     MIKPFDGWTNKFIFPPYDFTVATSMISNFHMPYSMLLMLVAAFGDYDYVMHAYSEAIK
                     EKYRFGTYGDALLII"
     gene            complement(1764..2447)
                     /gene="truB"
                     /locus_tag="PKN90_03150"
     CDS             complement(1764..2447)
                     /gene="truB"
                     /locus_tag="PKN90_03150"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNZ85309.1"
                     /translation="MNFIEGEILVFNKPLTWTSFALVHRVRAMICRKLKVKKLKVGHA
                     GTLDPLATGVMIICTGKATKRIEEFQYQTKEYIATLLVGATTPSFDLETKIDNVYPTE
                     HIKKETVQQVLAQFVGSIRQVPPVFSAVKVDGKRAYEYARNGEEVEIKAKELVIDEIE
                     LIDYSLPKITIRVVCSKGTYIRALARDIGVALQSGAHLVALERTCIGEYRIENCLTLD
                     DFQKIIDSE"
     gene            complement(2447..3304)
                     /locus_tag="PKN90_03155"
     CDS             complement(2447..3304)
                     /locus_tag="PKN90_03155"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNZ85310.1"
                     /translation="MSIVETVIIAIVEGLTEFLPVSSTGHMIITQALLGVESTEFVKA
                     FTVNIQFGAILAVVVLYWQRFFKLNDTSSNYSIANQSKWKQLLYKFDFYVKLFVAFLP
                     AACIGFFAGEYIDMMLESVEVVAVMLLVGGIFMLFIDKLFNKKSDNQAITYKRAWIIG
                     FAQCIAMIPGVSRSMASIVGGLATKLDRKNAAEFSFFLAVPTMAAAAGYTLMKLVMDE
                     RTSAMLVDNLVVLLIGNIVAFVVAIIAIKFFIGFITKYGFKAFGWYRIIVATVLLILL
                     VANVDLAIA"
BASE COUNT         1086 a          683 c          501 g         1101 t
ORIGIN      
        1 cttgtacttc ttctttgata acggcaataa cttcggcatt agtactcttt aaattaactt
       61 tatcctttag ttcataggta ctaaatatag ctgctaaacg ggcatcttta tctatttcct
      121 gaaaagcttc ttgaaacaaa tcaatgaaac ctttttggct tgttgcttga cgttggtgtg
      181 ctaactgtat ggctttagta aatgtttcgt cagtactatt acctgcaagg gtttttagaa
      241 tgtcgggcac tgacacttca atgataacat tcataccgcc ttttaggtct aagcctaagt
      301 ttaattcttt ctctctacac tctttcaagg tatagttaag ccacactttt tcagttgaaa
      361 gagaatctaa atagcggtac tcttcaatta catttccttg tgcaaactct tttgcttgtt
      421 tattgtaatg tgatgttaca acattaaacg acaagtaaaa aaggcacact aagaccaagg
      481 caatggtaaa agatagtaca agtcctttgt tttgcatgtt tgtattaaaa tttaaaatta
      541 atattcgttt gttttatatt tttgtgaaat agggtgcaaa tatagttttt tttttacaga
      601 aaagctatat tactgtaaaa taaaccgata taatccatac aatgagtgct ttaaataata
      661 agcaacgcat ctccataagt accaaaacgg tatttttctt ttatagcttc gctatatgca
      721 tgcattacat agtcataatc gccgaaagcg gctacaagca ttaataacat agaatatggc
      781 atatgaaaat tactaatcat gctagtggct actgtaaaat catacggagg gaaaataaat
      841 ttatttgtcc aaccatcaaa aggttttatc ataccattgg tactaacggc tgtttctaac
      901 gaacgtaaaa cggtggttcc gacagcacat acctgttgac gattttcttt tgcagcattt
      961 acaatttggc aagcctcatt cgttaccatc atttgctccg aatccatttt gtgcttagtc
     1021 aaatcttcca catcaatgtc gcgaaaattt cccaatccag cgtgtagagt tacgtttgca
     1081 aaatgtattc ccctaatttc catacgttta agcaattcgc gactaaaatg caacccagcg
     1141 gtaggggcaa ccacagctcc ttcatttttt gcatatatag tttgataacg ttcggcatct
     1201 tctggttctg cttctctatt caaagatcgt ggtagtggtg tttctcccaa agaatatagc
     1261 aattgcttaa atgtttcata attcccatcg tataaaaacc gcaaagtacg tcctctagaa
     1321 gtagtattat caattacttc ggcaactaat tcatcattat caccaaaata caacttattg
     1381 ccaatacgaa ttttgcgagc ggggtctacc aatacgtccc aaagtttaag ttctcggttt
     1441 aattctctta gtaagaacac ctcaatacgt gctcctgttt tttctttatt tccaaacata
     1501 cgtgctggaa acactttggt gtcgttaaat acaaatacat cgttttcgga aaagtattcg
     1561 tacacatttt taaatacttt gtgctcaatt tcaccagttt ttttattcaa caccaataag
     1621 cgcgattcgt cgcgaaattt tgtaggatgt gttgctatta gctcttcggg caacttaaat
     1681 ttaaattgtg aaagtttcat tttgattgtt tttgttatta ctatactcta ttaagatatg
     1741 tacaaattat gaagccacat atcttattct gaatcaatta ttttctgaaa atcatctaag
     1801 gtcaagcaat tttctattct atactcacca atgcacgtac gctctaaggc aacaagatga
     1861 gcaccacttt gcaaggctac tccaatatcc cgagccaaag ccctaatata tgtacctttg
     1921 ctacacacta cccttatggt aatttttggc aacgaataat cgattaattc aatttcatct
     1981 attaccaatt ctttggcttt tatctctact tcttctccat tacgggcata ttcgtacgct
     2041 ctttttccat caacttttac cgccgaaaaa acaggtggta cttgccttat tgagcccaca
     2101 aactgagcaa gaacttgttg taccgtttct ttctttatat gctcagtagg atacacatta
     2161 tctatttttg tttctaaatc aaacgacgga gtagttgctc caactagcaa cgtagcaata
     2221 tattcttttg tttgatactg aaactcttct atacgtttgg tagctttgcc tgtacaaata
     2281 atcattaccc cagtggctaa ggggtcgagt gtgcctgcat gaccaacctt taatttcttt
     2341 acctttagtt ttctgcatat catcgcacgc acacgatgta ctagagcaaa agatgtccac
     2401 gttagcggtt tattaaaaac caaaatctct ccttcaataa aattcattag gctatagcga
     2461 ggtcaacgtt tgcaactaac aagattagta aaacagtagc tactatgatt ctataccaac
     2521 caaatgcttt aaagccatac tttgtaataa acccaataaa aaatttgatg gcaataatag
     2581 ccacaacaaa agctacaata ttacctatca acaaaacgac caagttgtca accaacatag
     2641 ccgaagtacg ctcatccatt actaacttca tcaaagtata cccagctgcg gcagccatag
     2701 ttggtacagc caaaaaaaac gaaaattcgg cagcattttt tctatccaat ttagtagcta
     2761 aaccgcctac aatagatgcc atagaacgcg acactccagg tatcatggcg atacactgag
     2821 caaaccctat aatccaagct cttttatatg taatagcttg gttatctgat tttttattaa
     2881 ataatttatc aataaaaagc ataaaaatac cgcctacaag tagcattact gcaactactt
     2941 ctacactttc tagcatcatg tctatatact cgccagcaaa aaaaccgata cacgctgctg
     3001 gcaaaaaagc tacaaaaagt tttacataaa aatcaaactt atacaataac tgtttccact
     3061 tactttggtt agctatagaa taattagaag aagtatcatt cagtttaaag aaacgttgcc
     3121 aatataaaac aacaacagct aaaattgcac caaattgaat gtttacggta aatgctttta
     3181 caaattctgt actttcaaca cccaataatg cttgtgtaat aatcatgtgc cctgtagaag
     3241 atacgggcaa aaactctgtt agcccctcaa caatggctat aataacagtt tcaactatac
     3301 tcatgctttc gttttaggtg tatataaaat agcaaaaacg agaaataaaa atccaaaaaa
     3361 taccatcatt g
//
LOCUS       DAOPUR010000132         3346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_29049_length_3346_cov_3.322698, whole genome shotgun sequence.
ACCESSION   DAOPUR010000132 DAOPUR010000000
VERSION     DAOPUR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29049_length_3346_cov_3.322698"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="PKN90_03160"
     CDS             <1..1489
                     /locus_tag="PKN90_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNZ85311.1"
                     /translation="VHPFMRRDNYRAKNLAICGNFNLTNVDEIFNYISKKGQHPRKYA
                     DAFILLDQIGHRLDREVERVYALAKEEGKRGTDITYAIEEDIKIENVLQSSSWLWDGG
                     YVMCGLTGSGESFVLRDPWGIRPAFYYADEEIVVTASERPVIQTALNLTASQVKELNP
                     GEAMILDKAGNLRLVQILQPKIKQACSFERIYFSRGSDCDIYNERKALGENVVPGILK
                     SIDNDLKNTVFSFIPNTSEVAFYGMIQCLNERLNDIKNQQIRQYGRRLTSEMLDEILS
                     MSIRSEKVAIKDIKLRTFISEGNSRNELAAHVYDVTYGSIVPNQDNLVVIDDSIVRGT
                     TLKQSIIKILARLQPKKIVIVSSSPQVRYPDFYGIDMSNMDEFVAFRAAIALLKDNNM
                     QYVIDDVYKKCKQQENQHKEEIENYVKQIYAPFTAEQVSEKIVELLKPEDVQCPVELV
                     YQSLEGLLKSCPNHTGNWYFSGDYPTQGGKRLVNESFIRYYEKNY"
     gene            complement(1549..2022)
                     /locus_tag="PKN90_03165"
     CDS             complement(1549..2022)
                     /locus_tag="PKN90_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HNZ85312.1"
                     /translation="MYTWFECKVRYEKIVEGKTKKVTEPYLIEAFSYTEAEARVMQEL
                     SPYISGDYSITNIKRAKIAELFPNDLGDRWYTCKINIILLDESRGIEKKVANYILVQA
                     SAVLDAWQKLEIGMKGTLSDYVVVSIGESPIIDIFPYKKEEMKQVISSTNVSIEE"
     gene            2273..3274
                     /gene="gap"
                     /locus_tag="PKN90_03170"
     CDS             2273..3274
                     /gene="gap"
                     /locus_tag="PKN90_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNZ85313.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQERNDIQVVAVNDPFLDLDYLVYM
                     LQYDTVHGKFKGTAENKDGKLIVNGKEVKFYAEKDPAAIAWGAAGADYVVESTGVFTT
                     IDKAKAHLAGGAKKVVISAPSNDAPMFVCGVNLDAYKADMDIVSNASCTTNCLAPLAK
                     VINDNFGIVEGLMTTVHAATNTQKTVDAPSAKDWRGGRSILGNIIPSSTGAAKAVGKV
                     IPSLNGKLTGMAFRVPTADVSVVDLTVRLEKAASYDEIKATIKKASENELKGILGYTE
                     DSVVSTDFTTDSRTSIFDAGAGIALNGNFVKLVSWYDNEWGYSNKVLDLIAHMDSVK"
BASE COUNT         1086 a          529 c          630 g         1101 t
ORIGIN      
        1 tgtacatcct tttatgagaa gggataatta cagagcaaaa aatttagcta tttgtggtaa
       61 ttttaacttg accaatgtag atgaaatttt taattacata tctaaaaaag ggcaacatcc
      121 gcgtaaatat gccgatgcgt ttattctatt ggatcaaatt gggcatcgcc ttgatcgtga
      181 agtagaacgt gtatacgcct tagccaaaga agagggtaaa agaggtactg atataacata
      241 tgctattgaa gaagatatta agattgaaaa tgttttacag tcatcttctt ggctgtggga
      301 tggtggatat gttatgtgtg gtttaacagg aagtggcgaa agttttgtgc ttcgggatcc
      361 atggggaatt cgccccgcct tctattatgc tgatgaggaa attgtagtta ccgcctctga
      421 aagacctgta atacaaacag cattaaatct tacggcaagt caggtaaagg aactaaaccc
      481 aggagaagca atgatcctcg ataaagcagg aaacttgcga ctagtgcaaa tattgcaacc
      541 caaaatcaaa caagcatgtt cgttcgaacg tatttatttt tcgcgtggta gcgattgtga
      601 tatttacaac gaacgcaaag cgttgggtga aaatgtagtg ccaggtattc ttaaaagtat
      661 tgataatgat ttaaaaaata ctgttttttc atttattccc aacacttctg aggttgcttt
      721 ttatggaatg attcaatgtc ttaacgaacg tttgaatgat attaaaaatc aacaaattcg
      781 tcagtacgga cgacggttga cttctgaaat gttagatgaa atattatcca tgtctattcg
      841 ttcagaaaaa gtagcaataa aagatatcaa gttgcgtacg tttatttccg aaggcaatag
      901 tcgcaatgag ttagctgctc acgtgtacga tgttacgtat ggtagcattg tgcccaacca
      961 agataattta gtcgttattg atgatagtat tgtgcgtggt actaccctta aacaaagtat
     1021 cattaaaatc ctagcacgat tgcaaccaaa aaagattgta attgtatcct catcaccaca
     1081 agttcgttat cctgattttt atggtataga catgtcgaat atggacgagt ttgtagcttt
     1141 tcgtgcagcg attgcattgc tcaaagacaa taacatgcaa tacgtcattg atgatgtgta
     1201 caaaaaatgt aagcaacaag aaaatcagca taaggaagaa atagagaatt acgttaagca
     1261 aatatatgcc ccttttacag cagaacaagt ttctgaaaaa attgtagaat tactcaaacc
     1321 cgaagatgtg caatgcccag tggaattggt ataccaaagt ttagagggct tgttgaaatc
     1381 atgtccgaat catacaggta attggtattt ttctggagat tatcctacac aaggaggtaa
     1441 acgtttagtc aatgaatcgt ttattcgata ctacgaaaaa aattattaaa taaaaatagt
     1501 cctcgatgtt atatcgagga ctatttttat tactattgta tagttatctt attcttctat
     1561 tgatacattc gtcgaactaa ttacttgttt catttcctct tttttatacg ggaaaatatc
     1621 aattattgga ctctctccta tcgatacaac cacataatca gataaggtgc ctttcatgcc
     1681 tatttctaat ttttgccaag catctaacac ggcagaggct tgtaccaata tgtaattagc
     1741 tactttcttt tctatacctc tactttcatc aagcaagatg atattaattt tacacgtata
     1801 ccatctgtcg cccaaatcgt ttggaaaaag ttctgcgatt ttggcacgtt taatgtttgt
     1861 gattgaataa tccccactta tatatggcga taattcttgc attacgcgag cttccgcttc
     1921 tgtgtacgaa aaagcttcaa ttaaataagg ttctgttact tttttagttt ttccttctac
     1981 tattttctca tagcgcactt tacactcaaa ccatgtatac atattattgt tgtttttaat
     2041 tagagtgcaa aaatacagat aataatgagc ctcaaaagaa aattaggaaa atagttttcc
     2101 acaaaattgc agttacttgt ttacaagcct aatagcattt attaataaac caaatatggg
     2161 ttaacaaatc tctatttttt ttgaaaaaaa acaaacaatt ctttggttag taataaaaaa
     2221 aaagtaattt tgcatggttt aaaaaaataa cttaatttat aaataagaaa aaatgattaa
     2281 agtaggtatt aatggatttg gccgtatcgg acgtttagtg ttccgtgcag ctcaagaaag
     2341 aaacgacatt caagttgttg cagtaaatga cccatttttg gatcttgact atttagttta
     2401 tatgttgcag tatgatactg tacacggtaa atttaaaggt acagctgaaa ataaagatgg
     2461 taagttaatc gtaaacggta aagaagtaaa attctatgcc gaaaaagatc ctgctgctat
     2521 tgcttggggt gctgctggtg ctgattatgt tgttgaatca acaggtgttt tcactactat
     2581 cgacaaagca aaagctcact tagcaggtgg tgctaaaaaa gtagttatct ctgctccttc
     2641 gaatgatgct cctatgttcg tgtgtggtgt taacttagat gcatacaaag ctgatatgga
     2701 tatcgtatca aatgcatctt gtactactaa ctgtttggct cctttggcta aagttattaa
     2761 tgataatttt ggtattgtag aaggtttgat gacaactgta cacgctgcta ctaatactca
     2821 aaaaacagtt gatgctcctt ctgctaaaga ttggagaggt ggtcgttcaa tcttaggtaa
     2881 tattatccca tcttctacag gtgctgcaaa agcagtaggt aaagttatcc catcattgaa
     2941 tggtaaactt acaggtatgg ctttccgtgt accaacagct gacgtttctg tagttgactt
     3001 aactgtgcgt ttagaaaaag ctgcttcgta cgacgaaatt aaagctacta tcaaaaaagc
     3061 ttctgaaaat gaattgaaag gtattttagg gtatactgaa gactcagtag tttctactga
     3121 ctttactacc gactctcgta cttcaatttt tgatgcaggt gcaggtattg ctcttaatgg
     3181 taactttgtg aaattagtta gctggtatga caatgagtgg ggttattcta acaaagtgtt
     3241 agatttaatt gcacacatgg attcagttaa ataacactaa tttttttagt aaatgcaatt
     3301 gggtagtaaa ttaatttact acctaatttt ttttgctata gggata
//
LOCUS       DAOPUR010000133         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_29312_length_3325_cov_2.948318, whole genome shotgun sequence.
ACCESSION   DAOPUR010000133 DAOPUR010000000
VERSION     DAOPUR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29312_length_3325_cov_2.948318"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKN90_03175"
     CDS             complement(<1..216)
                     /locus_tag="PKN90_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HNZ85314.1"
                     /translation="MKYIIILGDGMADEPIAKLGNKTPLQFANTPYIDKVAKLGCSGM
                     LHTIPDGFHPGSEIANLSVLGYKVADVF"
     gene            complement(229..1278)
                     /locus_tag="PKN90_03180"
     CDS             complement(229..1278)
                     /locus_tag="PKN90_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNZ85315.1"
                     /translation="MISKIILEDLISQTKKAIDKSTSIVIVSHVNPDGDAIGSSLGLY
                     HFLFSQGKETRIIMPNSFPNFLNFLPDSSDILIYDDYQDEAIDLIESADLVFCLDFNQ
                     LKRVGAMEDIIKNCKATKILVDHHPFPDTFANITISHPEIASTSELIFRMICRMGYFT
                     EMNIHSAECICTGIMTDTGAFSYNSNSPEIYTIISELIKKGVDKDALHRKLYNNYSAQ
                     RMQLMGYLLSQKMKIYAEYGTAIMVLSLAEQETYHHKKGDSEGFVNLPLSIHGITFSI
                     FLKEETDAIKMSFRSQGNFPSNTFANRYFNGGGHLNASGGEGLGTIEETVTYIESILP
                     AFYQEWVDNNNNKEI"
     gene            complement(1323..>3325)
                     /locus_tag="PKN90_03185"
     CDS             complement(1323..>3325)
                     /locus_tag="PKN90_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNZ85316.1"
                     /translation="IGIFISSVVPLSLTIGYILLFLSIIGVIISNVIDKRKTLSYSFR
                     WIYGVSIIGIFMSLGMLLYQHKIQELSLELPDNKKTFIATISESPIEKPKSIMTVVTI
                     AVPEINKTHRAILYLEKDSASKTVQYGDYLLVNTLLQKPRNTGNPYEFDYSTFLLYKG
                     ITATSYINKGSWKQIKKTSSFSVTKHAINTRNKLLNIYKKYGITDNEFGIVAALTVGY
                     KEGLSKETKQSFSITGGSHVLAVSGLHVGIIYLALGFMLGFMAKSKQGIVGKQIILIA
                     SIWAYAFICGLPPSVVRASIMISLICISEILNRKSATYNAVFVSGFCMLIYNPLYLFD
                     VGFQLSYMAVLSILYFQPRINNIFSPNHIVIKWLWSLTAVSIAAQIGTAPISLYYFHN
                     FPTYFLLTNIIVIPAATIIIYLSIVLLLLSSWVKIGLIVAWLLKSCVQIISIGIHFIE
                     HLPGASIPDIWIPIWFMPIVFLSLVSTILFIRTQRSRYFFLIFYLSILSISIAIWNRF
                     QTLQNKELIVYANNPSETNFNIHIISGESNTLLADTIYKANLEQYVNTVTHKKHLNPA
                     RFEHIDMKNSFGCIDTLTYFVYTNPTKWKPIDISIPVDIVFVYEKRMCHVTEITSHVS
                     PTYIVFGENINGKQLQILKEECEKKSINYHSLYEKGAFILPL"
BASE COUNT         1086 a          545 c          558 g         1136 t
ORIGIN      
        1 aaacacatca gctaccttgt atccgagtac gcttaaattg gcaatttcgc tacctggatg
       61 aaagccatca ggtatggtat gtagcatgcc actacaacca agtttagcta ctttatcgat
      121 atacggagta tttgcaaatt gcaagggtgt tttgtttcct aatttagcta ttggctcatc
      181 agccattccg tctccaagta tgataatata tttcatgtaa gtataagttt atatttcttt
      241 gttattgtta ttatcaaccc attcctgata aaatgcaggt aatatggatt caatatacgt
      301 aactgtttct tcgattgtac ctaaaccttc acccccagag gcatttaaat gaccaccacc
      361 attaaaataa cggttggcaa acgtgttgct tggaaaatta ccttgcgagc ggaacgacat
      421 ttttatagcg tctgtttctt ctttcagaaa aatagaaaat gtgatgccat gtatggataa
      481 tggcaaatta acaaaaccct cgctatctcc ttttttgtgg tgataggttt cttgttctgc
      541 caaagacaaa accatgattg cagttccgta ttcagcatag attttcattt tttgcgacaa
      601 taaatatccc attaattgca tccgttgtgc tgaataatta ttatataatt ttctgtgtaa
      661 tgcatcttta tcaacaccct tttttatcaa ttctgaaata atggtataga tttcgggact
      721 attggaattg tacgaaaaag ctcctgtatc ggtcataata cccgtacaaa tgcattctgc
      781 tgaatgaatg ttcatttcag taaagtatcc catcctacaa atcatacgaa aaattaactc
      841 acttgtggag gcaatttcgg gatgtgatat agtaatgtta gcaaaggtat ctggaaatgg
      901 gtgatgatct actaatattt ttgttgcttt gcaatttttt attatatctt ccattgcacc
      961 tacacgtttt agttgattaa aatccagaca aaaaactaaa tcggcagatt caattaagtc
     1021 gatagcttca tcttgataat cgtcatatat caagatatca ctactgtcgg gtaaaaagtt
     1081 aaggaaatta gggaaagaat ttggcataat aatacgagtt tcttttccct gagaaaacaa
     1141 aaaatgatac agccctaaag aactaccaat ggcatctcca tcagggttga catgagatac
     1201 aatcacaata ctagtggatt tatcaattgc tttttttgtt tgtgaaatta aatcttcgag
     1261 aataattttt gatatcatat tatcactatt agtaaatcta ctgcaaaaat agtaatactt
     1321 tgttatagtg gaagtataaa tgctcctttt tcgtacaacg aatgatagtt aatgctcttt
     1381 ttttcacatt cttctttcaa tatttgtagc tgttttccat ttatattttc tccaaataca
     1441 atgtaggtgg gagatacatg ggacgtaatt tctgttacat gacacattct tttttcatat
     1501 acaaaaacaa tatctactgg tatagatatg tcaatgggtt tccattttgt ggggttggta
     1561 tacacaaagt aagtgagtgt atcaatacaa ccaaaagaat tcttcatatc gatgtgttcg
     1621 aaacgtgctg gattcaagtg tttcttatgg gttactgtat tcacatactg ttcgaggttt
     1681 gccttatata tagtatctgc taacagtgta tttgattctc cagaaataat atgtatatta
     1741 aaattcgttt ctgaagggtt gtttgcatag acaattagtt ctttattttg caatgtttga
     1801 aatctattcc agattgcaat cgaaatggat aaaatagata aatagaagat taaaaagaaa
     1861 tatcttgatc gctgagtgcg aataaaaagt atggtactaa caagtgataa aaaaacgata
     1921 ggcataaacc aaatcggaat ccaaatgtca ggtatgcttg ctcctggtaa atgctcaata
     1981 aaatgtatcc caattgaaat aatttgcaca caacttttta gtaaccatgc aactattaat
     2041 ccaatcttta cccaagaaga aagcaataaa agtactatag aaagataaat aataattgta
     2101 gcagctggaa tgactataat gtttgtaagt aaaaaatatg ttggaaagtt atgaaaataa
     2161 tataaactaa taggagctgt tccaatttgt gctgcaatag agactgccgt cagactccat
     2221 aaccatttga ttactatatg attgggggaa aatatgttat tgatacgagg ctgaaaatac
     2281 aagatactta atacagccat atagcttaat tgaaaaccta cgtcaaataa gtataacggg
     2341 ttgtaaatca gcatacaaaa cccactaaca aatacagcat tgtatgtagc cgattttctg
     2401 ttcaatattt cgctgataca aattaaggat atcatgattg atgctcttac aactgatgga
     2461 ggaagaccgc aaataaaagc atacgcccaa atacttgcta tgagtataat ttgtttgcct
     2521 actatccctt gtttgctttt tgccataaat ccaagcataa acccaagtgc aagataaata
     2581 ataccaacat gtaagccgct tacagctaat acatgagagc ctccagtaat cgaaaaactt
     2641 tgttttgtct ctttagataa tccttcctta tatcccaccg tcagtgctgc aacaatacca
     2701 aattcattgt ctgttatacc atatttttta tagatattga gcaatttatt gcgggtattg
     2761 atagcatgct tcgtaactga aaaagaggat gtttttttta tttgtttcca acttccttta
     2821 ttgatgtatg atgtggcagt gatgcctttg tataataaaa atgttgaata atcaaattcg
     2881 tatgggtttc ctgtatttcg cggtttttgg agtagtgtgt tcactagcag ataatcacca
     2941 tattgtactg ttttcgatgc actgtctttt tctaggtata gtatagctct atgtgtttta
     3001 tttatttctg gaactgctat tgtaacaacc gtcattattg attttggctt ttcgatagga
     3061 gattcactaa ttgtggcaat aaatgtcttt ttgttgtcag gaagttcaag gcttaattct
     3121 tgtattttat gctggtataa gagcattcca aggctcataa atattcctat aatggataca
     3181 ccatatatcc accgaaaaga atagctcaac gtttttcttt tgtctattac attggaaata
     3241 atcactccga tgattgacaa aaaaagcaat atgtaaccaa tagtgaggct taagggaact
     3301 acagaactta taaatatgcc gataa
//
LOCUS       DAOPUR010000134         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_29536_length_3306_cov_2.834205, whole genome shotgun sequence.
ACCESSION   DAOPUR010000134 DAOPUR010000000
VERSION     DAOPUR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29536_length_3306_cov_2.834205"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKN90_03190"
     CDS             <1..419
                     /locus_tag="PKN90_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ85317.1"
                     /translation="DAYGEYEDEKIIELPKNIFEVDGAIDEEMIAEGNMVPLVDSEGN
                     RINASIVSVGEDIVTVDINHPLAGEDLHFIGKVIEMREATEEELKAVFSSGCGCGSGG
                     CDSGDCGSGDCGSGGCGCSSGECDSDDMYGCNCGCN"
     gene            456..1538
                     /gene="aroC"
                     /locus_tag="PKN90_03195"
     CDS             456..1538
                     /gene="aroC"
                     /locus_tag="PKN90_03195"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNZ85318.1"
                     /translation="MINSFGTIFKLTSFGESHGKALGGVIDGCPAGVSIDEDFIQQEM
                     DRRRPGQSRLTTARNEPDSVEFLSGIFEGKTTGTPIGFVVYNQNQRSEDYDHLKDVYR
                     PSHADYVYQEKYHNRDYRGGGRSSARETVSRIVAGAIAKLALKQIMQGIDIKAYTAQV
                     GDIALDKPYQAYDLSTIENNVVRCPDAKVAEKMIELIAETKKQGDTIGGVITCVVKGV
                     PLGLGEPVFGKLHALLGSAMLSINAVKGFEYGMGFAGVSSNGSKVNDTFYVDNGVVKT
                     KTNNSGGIQGGISNGQDIYFNVAFKPVATILQEQEVLTRNLEVQVQKVRGRHDSCVLP
                     RAVPIVEAMAAMVIFDAYLMAKVRTL"
     gene            1548..2495
                     /locus_tag="PKN90_03200"
     CDS             1548..2495
                     /locus_tag="PKN90_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNZ85319.1"
                     /translation="MTNETTNKQWAIVVNPHAGGKKAFQFWQKLSVALREQGIGYDIA
                     FTEGPFHAVQLAKKFRHQLYEQFLVIGGDGTLSEVVHGIFSCSVPPNPTITLSIVPIG
                     TGNDWARFWGITPTIERVVQSLLNHKTQTIDIGQISFVTYQGIPQTRYFINALGEGFD
                     AKVTKLTTYLGSIFKGKSWLYSLSVFIVPFVYRAKPIHIEFDNGLLVQKKIYTLSCGN
                     GCYTGGGIKQTPHAVPTDGLFDVMMVESPSLWGIITGIHALFKGKLMQHKLVQSTQTK
                     HFTISSDYPLCMEIDGISLPETYQATISILPKAVRFIVV"
     repeat_region   2569..2730
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2569..2597
                     /rpt_unit_seq="cttttaatcagactaatgtagaattgaaa"
     gene            3078..>3306
                     /locus_tag="PKN90_03205"
     CDS             3078..>3306
                     /locus_tag="PKN90_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85320.1"
                     /translation="MKSGEIYTVYRVLDIEDGYLTFKRNKDSNISTIDASLVKTIGFQ
                     PKQLRDATDAYDKAYIIEGSFYEKNKKLSESA"
BASE COUNT         1028 a          569 c          710 g          999 t
ORIGIN      
        1 atgatgctta tggcgaatac gaagatgaaa aaatcattga attgcctaaa aatatatttg
       61 aagtagatgg tgctattgac gaagaaatga tagccgaagg caatatggta ccattggtag
      121 attcagaggg taatcgtatc aatgcatcta tcgtatctgt aggagaagat atcgttaccg
      181 tagatatcaa ccatccttta gctggcgaag atttgcattt tattggcaaa gtgattgaaa
      241 tgcgtgaagc tactgaagaa gaattgaaag cggtattttc ttcgggatgt ggttgcggta
      301 gcggaggttg tgatagtggc gattgtggta gtggcgattg tggtagtggt ggatgtggtt
      361 gctcatcagg agaatgcgat agcgacgata tgtatggctg taattgcggt tgtaactaac
      421 ccttttttat aatttttacc aaaagaaaaa taagaatgat aaattcattt ggaaccattt
      481 ttaaattaac atcgtttggc gagtcgcacg gaaaagcatt gggtggggta attgatggtt
      541 gccctgcagg tgttagtatc gacgaggatt ttatccaaca agagatggat agacgacgtc
      601 ctggacagtc gcgcttaact actgcacgca acgaacccga tagtgtagaa tttttatcag
      661 gtatttttga aggaaaaaca actggtacgc ctataggatt tgtggtgtac aaccaaaatc
      721 aacgctcaga agattatgat catcttaaag atgtgtatcg tccttcgcat gccgattatg
      781 tgtaccaaga aaaataccac aacagagact accgtggagg tggacgttcg tcggctaggg
      841 aaactgttag ccgaattgta gcaggtgcca ttgcaaaatt agcactcaag caaattatgc
      901 aaggtattga tatcaaagca tacaccgcac aagttgggga tattgcctta gacaaacctt
      961 accaagcata cgatttatct actatcgaaa acaatgtagt acgttgtccg gatgccaaag
     1021 tggctgaaaa aatgattgaa ttgatagccg aaaccaaaaa acaaggcgat accattggtg
     1081 gcgttattac gtgtgtagta aaaggtgtac cattggggtt gggcgagcct gtatttggta
     1141 agctgcatgc tttgttgggt agtgccatgt tgtctatcaa tgctgtaaaa ggctttgaat
     1201 atggtatggg atttgctggc gtatcgagca atgggtcgaa ggtaaacgat accttttatg
     1261 tagataatgg ggtagtgaaa accaaaacca acaactctgg tggcatacaa ggtggtattt
     1321 ccaacggaca agatatttat tttaatgtag cctttaaacc cgtagctact attttgcaag
     1381 aacaagaagt attgacccgc aacttagaag tacaagtaca aaaagtacgc ggcagacacg
     1441 attcgtgcgt tttaccacgt gcagtaccca ttgtagaagc catggcagcc atggtaattt
     1501 ttgatgcgta tttaatggct aaagtgcgta cgctatagtg gttttggatg acaaacgaaa
     1561 caacaaataa gcaatgggca attgttgtca atccgcatgc tgggggcaaa aaagcttttc
     1621 agttttggca aaaattgtct gttgctttac gtgagcaggg catagggtat gacattgcgt
     1681 ttaccgaagg accgtttcat gccgtacagt tggctaaaaa gttccgtcat cagttgtacg
     1741 aacaattctt agtcattggt ggtgatggta ccttgagcga ggttgttcat ggtattttta
     1801 gttgttctgt tcccccaaat cccaccatta ctttatctat tgtacccatt ggtacgggca
     1861 acgattgggc acgtttttgg ggtattacgc ctactatcga aagggtggta cagtccttac
     1921 tcaaccataa aacccaaacc attgatatcg gtcaaatttc atttgtaacc tatcagggca
     1981 taccacaaac acgttatttt atcaatgccc tgggcgaggg ttttgatgcc aaggtaacca
     2041 agttaaccac ctatttgggt tccattttta aaggaaaatc gtggttgtat agcctgtcgg
     2101 tgtttattgt tccttttgtg tatagagcca aacctataca tattgagttt gataacggat
     2161 tgctggtaca gaaaaaaatc tacaccttat cctgcggaaa cggttgctat acaggtggtg
     2221 gcattaagca aacgccacat gctgtaccta ccgatggatt gtttgatgtg atgatggtag
     2281 aaagtccttc gttgtggggt attattacag gtattcatgc cttattcaaa ggcaaactta
     2341 tgcaacacaa attagtacaa agtacccaaa ccaagcattt taccatttct tccgattatc
     2401 ccttatgtat ggaaatagat ggcatctcac tacccgaaac ttaccaagcc accatcagca
     2461 tactgcccaa ggctgtacgg tttattgttg tgtagttatt gtgtatggat atccttgaac
     2521 gaggtagatt ttacctctgt agttttacta tatgctacct ttacactact tttaatcaga
     2581 ctaatgtaga attgaaattt acttctaagg ctaatctatt cgcagtaatc tgagctttta
     2641 atcagactaa tgtagaattg aaatttggaa agatttgcag gttgtactat tgatacttcc
     2701 ttttaatcag actaatgtag aattgaaagc aaatcacaac acttaaaata ggagatacaa
     2761 aaaatgttta gttgtttatc aagtcaaaga aacaatatct tgaattgaaa atcggcgtag
     2821 cgaaagcaac ttacaaccaa atattaaatt gttagaagtc gtacttatta atgcaaattc
     2881 ttagaacctc atttttgtag ctaaaatctc cttgtaatta ctacacaaat catcacactc
     2941 ttaacttgaa taagttaaaa tttttatata catttgcaca ccttgttcta gtaactattc
     3001 ttatgaaaaa aattacctaa cttcttccat tccttctctt tacttttgtt gcctatagcc
     3061 aaaatgatac tatttttttg aaaagcggag aaatatatac agtttataga gtacttgata
     3121 ttgaagatgg ctatttgacg tttaaacgga ataaagattc aaacattagt actattgatg
     3181 cctcattggt aaagaccatt ggttttcaac caaaacaact aagagatgca acagacgcgt
     3241 atgataaggc gtatatcatc gaagggagct tttatgaaaa gaacaaaaaa ttatcagagt
     3301 cagcct
//
LOCUS       DAOPUR010000135         3303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_29576_length_3303_cov_3.463054, whole genome shotgun sequence.
ACCESSION   DAOPUR010000135 DAOPUR010000000
VERSION     DAOPUR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29576_length_3303_cov_3.463054"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /gene="rpsM"
                     /locus_tag="PKN90_03210"
     CDS             complement(<1..108)
                     /gene="rpsM"
                     /locus_tag="PKN90_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNZ85321.1"
                     /translation="MAIRIVGVDLPQNKRGEIGLTYIYGVGRSSANKILE"
     gene            complement(134..250)
                     /gene="ykgO"
                     /locus_tag="PKN90_03215"
     CDS             complement(134..250)
                     /gene="ykgO"
                     /locus_tag="PKN90_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNZ85322.1"
                     /translation="MKVRASLKKRTPECKIVRRKGRLYVINKKNPKYKQRQG"
     gene            complement(269..487)
                     /gene="infA"
                     /locus_tag="PKN90_03220"
     CDS             complement(269..487)
                     /gene="infA"
                     /locus_tag="PKN90_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNZ85323.1"
                     /translation="MAKQTAIEQDGTIIEALSNAMFRVELENGHEITAHISGKMRMHY
                     IKILPGDKVRVEMSPYDLTKGRISFRYK"
     gene            complement(499..1269)
                     /gene="map"
                     /locus_tag="PKN90_03225"
     CDS             complement(499..1269)
                     /gene="map"
                     /locus_tag="PKN90_03225"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNZ85324.1"
                     /translation="MIYLKTHDEIELMRESNLLVSRTLAEVAKNIRVGIKTLELDAIA
                     EAFIRKNGALPAFKGYSGFPNTLCVSVNEQVVHGIPSDYMLQEGDIVSVDCGVCKNGF
                     YGDSAYTFLVGEVDNNVKSLLKVTKEALYKGIEQAIHGKRIGDIGNSVQEYCEKNGYS
                     VVREMVGHGIGKHLHESPEVPNFGKKGQGIVLKEGMVIAIEPMINLGKKEIAFKRDGW
                     TTVTVDGLPSAHFEHTVAIKKGRADILSTFDYIEEVLN"
     gene            complement(1298..2635)
                     /gene="secY"
                     /locus_tag="PKN90_03230"
     CDS             complement(1298..2635)
                     /gene="secY"
                     /locus_tag="PKN90_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNZ85325.1"
                     /translation="MKRFIETIKNILKIEELKSRLLLTFLLVVIYRFGSYVALPGVDV
                     NALSALSQQTEGGLMMLLDMFSGGAFSNASVFALGIMPYISASIVVQLLGMAFPYFQK
                     LQREGESGRKKMNQITRYLTVGILLVQGPAYLGNLKMQMGNVFPEGWYFIFSSVIILA
                     AGSMFVLWLGERITEKGLGNGISFIILVGIIAKFPGAIIGEFSSRLNDASGGLVMFLL
                     EIVFLLVVIAASILLVQGTRKIPVQYAKRVVGNQQYGGVRQYIPLKVNAAGVMPIIFA
                     QAIMFVPASLSGLFATDGNVSAFAQAFMDHTSFWYNLVFALMIIAFTYFYTAVTINPT
                     QMAEDMKRNNGFIPGIKPGKKTAEYLDSVMSRITLPGSFFLAIVAILPAFAGLLGISF
                     SFAQFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKNGKIKGRTGSIAAY"
     gene            complement(2640..3086)
                     /gene="rplO"
                     /locus_tag="PKN90_03235"
     CDS             complement(2640..3086)
                     /gene="rplO"
                     /locus_tag="PKN90_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNZ85326.1"
                     /translation="MNLSNLKPAGGSVKTCKRVGRGSGSGLGGTSTRGHKGAKSRSGY
                     SKKVGFEGGQMPIQRRLPKFGFNNVNRIEYKAINLATLQQLAEEKKLEKIGIAELLEG
                     GFIARKHNVKILGNGVLTAKIEVTAHAFSKSAIEAIEKAGGSVIKM"
     gene            complement(3123..3299)
                     /gene="rpmD"
                     /locus_tag="PKN90_03240"
     CDS             complement(3123..3299)
                     /gene="rpmD"
                     /locus_tag="PKN90_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNZ85327.1"
                     /translation="MATIKVKQVKSRIKCPKVQKLTLDALGLKKMNRVVEHEATPQIL
                     GMINKVKHLVVVEE"
BASE COUNT         1060 a          721 c          408 g         1114 t
ORIGIN      
        1 ttccaaaatt ttattagcac tacttctacc tactccgtag atataggtta aacctatttc
       61 acctctctta ttctgaggca aatctactcc gactattctt atcgccataa taaactttta
      121 tatttgtttt taattatcct tgacgttgtt tatactttgg atttttttta ttaatcacat
      181 aaagacgtcc ctttctcctt acaatcttac attcaggagt acgctttttt aacgatgctc
      241 ttactttcat tattcttata tttaatattt atttgtacct aaaagaaatc cttccttttg
      301 ttaaatcata cggtgacatt tccacacgca ctttatcccc aggtaaaatc ttaatataat
      361 gcatgcgcat tttaccagaa atatgtgctg ttatttcatg tccattttct aactctacac
      421 gaaacatcgc atttgacaat gcttcaatta ttgttccgtc ttgctctatt gcagtttgtt
      481 ttgccatgaa ataaattact aatttaatac ttcttcaata taatcaaacg ttgagagtat
      541 gtcagctctc ccttttttaa tagctacagt atgctcaaaa tgtgctgagg gtaacccatc
      601 tacagttaca gtagtccaac catctcgttt aaatgcaatc tctttttttc ctaaattaat
      661 cattggttca attgcgatta ccatcccttc ttttagaaca atgccttgac cttttttccc
      721 aaaatttgga acttctggag attcatgtaa atgcttacct ataccatgac caaccatttc
      781 acgcactaca gaatatccat tcttttcaca atactcttga accgaatttc ctatatcacc
      841 aattcttttc ccatgtatcg cttgttcgat tcctttatac aaagcttctt ttgtaacttt
      901 caacaaactc ttaacgttat tatcaacttc accaactaag aacgtatacg ctgaatctcc
      961 ataaaatcca ttcttacaaa ctccacaatc cacagaaaca atatctcctt cttgtagcat
     1021 ataatcagaa ggaataccat gtactacctg ttcatttaca gaaacacaca aagtattagg
     1081 aaatccacta tatcctttaa atgcaggcaa agctccattt ttccgtataa atgcttctgc
     1141 tatagcatcc aactcaagtg tttttatccc tactcgaata tttttagcta cttctgccag
     1201 cgttctactc actaatagat tgctttctcg catcagctct atttcatcat gtgtttttaa
     1261 atatatcatc aaacaacgtt gtttcaacaa accagtgcta gtaagcagct attgatcctg
     1321 ttcttccttt aattttaccg tttttcatca aaccatcata atgcctcatc aacaaatggc
     1381 tctctatctg ttgtaaagta tctaatacaa cgcctaccaa aatgagtaat gacgttccac
     1441 caaaaaattg tgcaaaagaa aaactaatcc ctaacaagcc agcaaaagca ggcagaatcg
     1501 caacaattgc taagaaaaaa gacccaggca aagtaatacg agacataaca gaatctaaat
     1561 attctgctgt ttttttacct ggtttaatgc caggaataaa tccattatta cgtttcatgt
     1621 cttctgccat ttgagtagga ttaatcgtta cagcagtata aaaataagta aatgcaataa
     1681 tcataagagc aaatactaaa ttataccaaa aacttgtatg atccataaat gcttgtgcaa
     1741 aagcactgac atttccatct gtagcaaata gtcctgacaa tgatgcaggc acaaacataa
     1801 ttgcttgtgc aaaaatgatt ggcatcacac cagcggcatt aacttttaac ggaatatact
     1861 gacgaacgcc cccatattgt tgatttccaa ccacacgctt tgcatactgt acgggtatct
     1921 ttctagttcc ctgtacaagt aaaatacttg cagcaataac aaccaataaa aagactatct
     1981 ctaataaaaa cattactaaa ccaccagaag catcatttaa tcgagaagaa aattcgccta
     2041 ttatagctcc tggaaacttc gcaataatcc caactaaaat aataaatgaa ataccatttc
     2101 ctaaaccttt ttctgttatt cgttctccta gccatagaac gaacatactt cctgcagcta
     2161 aaataataac agaagaaaat atgaaatacc aaccctcagg aaacacattt cccatttgca
     2221 ttttaagatt tcctaagtaa gcaggacctt gtactaaaag aataccaacg gttaaataac
     2281 gtgtaatttg gttcattttc tttcttccac tctctccctc tctttgtaat ttttggaaat
     2341 aagggaaagc catgccaagt aactgcacta cgatagaagc agaaatataa ggcatgatac
     2401 ccaatgcaaa tacagaggca tttgaaaaag ctccacctga aaacatatca agcaacatca
     2461 tcaaaccacc ttcggtttgt tgacttagtg cagataaagc attcacatca acacctggta
     2521 gagctacata tgaaccaaaa cgatagatta ccactaacaa aaaggttagt agtaatctag
     2581 attttaattc ttcaatcttt aaaatatttt taattgtctc tatgaacctt ttcatgttct
     2641 tacattttaa ttacagagcc accagctttt tcaatagctt caattgcaga ttttgagaaa
     2701 gcatgagcag ttacctcaat ttttgctgtt aaaacaccgt tacctaaaat cttaacattg
     2761 tgtttacgtg caataaagcc cccttctaac aactctgcta tacctatttt ttctaacttc
     2821 ttttcttcag ctaattgttg caatgttgct aaattaatag ctttatattc aattctattt
     2881 acattgttaa agccaaattt tggtaagcga cgttgaatag gcatctgacc tccttcaaac
     2941 ccgacttttt tagaatatcc agaacgagac tttgctccct tatgacctct agtagatgta
     3001 ccacccaagc ctgaacctga accacgtcca actcttttac aggttttaac agaacctcct
     3061 gcaggtttta aattactcaa attcattgtt atacagtttt ttatgttgtt gataaaggat
     3121 tattattcct ccacaaccac caagtgctta actttattaa tcattcctaa tatttgcggt
     3181 gtagcttcat gctcaaccac tctattcatt ttttttaatc ccaaagcatc caatgtcaat
     3241 ttttgaactt ttggacattt aattctactt tttacttgct ttacttttat agttgccata
     3301 gtg
//
LOCUS       DAOPUR010000136         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_29946_length_3273_cov_2.709447, whole genome shotgun sequence.
ACCESSION   DAOPUR010000136 DAOPUR010000000
VERSION     DAOPUR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29946_length_3273_cov_2.709447"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKN90_03245"
     CDS             complement(<1..615)
                     /locus_tag="PKN90_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNZ85328.1"
                     /translation="MNALTKTDFNFDKQKSVYHGKVRDVYNINDELLVMVVSDRISAF
                     DVVLPKGIPYKGQMLNQIAAKFLDATADIVPNWKLATPDPMVTVGVHCDSYPVEMIVR
                     GYLCGSAWRAYQKGVREICGVKIPDGMRENQRFAEPIITPTTKADQGFHDEDISKEEI
                     LKQGLVSAEDYALLEKYTMQLFLRGTEMALERGLILVDTKYEFGK"
     gene            complement(641..1609)
                     /locus_tag="PKN90_03250"
                     /pseudo
     CDS             complement(641..1609)
                     /locus_tag="PKN90_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            1987..2964
                     /locus_tag="PKN90_03255"
     CDS             1987..2964
                     /locus_tag="PKN90_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ85329.1"
                     /translation="MNKCMEKPYVVGIDIGGTNTVFGIVDARGSVLATNSIKTQKHTV
                     LEDYIQELYDELTKLIADNTHISKIKGIGIGAPNANYFNGTIEQAPNLPWKGIIPFAD
                     KIQKKFGLPVAITNDANAAAIGEMIYGAARGMKNFIMITLGTGVGSGIVANGSLVYGS
                     DGFAGELGHTTMRRNNGRLCGCGLTGCLETYASATGVARSAREFLETQKDVPSRLRDI
                     PADEITSKDVYEAAVAGDKLAQEVFAFTGQILGEAFADFIKFSSPEAIIMFGGLSKAG
                     ELLLRPIRESMEKNVMPIFRNKVKLLFSELKEADAAVLGASALAWEIKD"
BASE COUNT          960 a          584 c          626 g         1103 t
ORIGIN      
        1 tttgccaaac tcatattttg tatctaccaa aattaatcca cgctctaaag ccatttcagt
       61 acctcttaga aatagttgca tggtgtattt ttcgagcaaa gcatagtcct cagccgacac
      121 aagtccttgt tttagaatct cttctttcga tatatcttcg tcgtgaaaac cttgatctgc
      181 ttttgtggta ggagtaataa ttggctcagc aaaacgttga ttttcacgca tgccatcagg
      241 tatttttaca ccacaaatct cacgtacacc tttttggtag gctctccatg cactgccaca
      301 caagtagcca cgcacaatca tttctacagg atacgaatcg caatgcacac ccactgttac
      361 cattgggtcg ggtgtagcca atttccagtt tggcacaata tcagcagtag catccaaaaa
      421 tttggcagca atttggttca gcatttgtcc tttgtaagga atacctttgg gcaatactac
      481 atcaaaagcc gaaatacggt cgcttactac cattaccaat aattcgtcgt taatgttata
      541 cacatcacgt actttgccat gatacacgct tttttgcttg tcgaagttga aatcagtttt
      601 tgttagtgca ttcattgttg ttagggttta gagtgggttc ttatttagtg tttatctctt
      661 tttgttgttt tatttcaaga gcatgttgtt cgtatgcctc aataatgcga cttaccaact
      721 tgtgtcgtac aatatcttgt ttggtaaaat gaatatgagc tacaccttct acgtggcgta
      781 aaatttacat agcgtgtatc atcccagaca cttgcgatga gggtaagtca atttgcgtaa
      841 tatcaccagt gataatcatt ttagagtgca aacctaagcg ggtaagaaac attttgattt
      901 gttgtgtgct cgtattttga gcttcgtcca aaataataat ggcatcgttg agtgtgcgtc
      961 cacgcataaa agccaacgga gctatttgaa tgatgttgtt ttcgatgtaa tcctttaatt
     1021 ttactgcagg aatcatatct tgcaaggcat cgtacagagg ttgtaagtaa gggtcaattt
     1081 tttctttcat atccccaggc aagaaaccca acttttcgcc cgcttcaaca gctggtctgc
     1141 ttaaaataat tttttttgct gttttatttt tcaatgctct cactgctaaa gcaatggctg
     1201 tatacgtttt tcccgatccg gcaggtcctt cggcaaatag catatcgtgt gtatcaaata
     1261 attctaccaa cttttgctga ttttcagtgc gagctgttat tggttttcca ttcagcccat
     1321 gcaaaattaa attatttggc ttgcatacaa ggggtgtttc tcctttgatg atgttaataa
     1381 taacttcttc tgataagttg ttgtattcgg taatatgctg ttctattttt gaaatttttt
     1441 cggcaaacag agctatttcg tctttatcgc ccattgcctt aatagcattt cctcgtgcta
     1501 taatacgtac ctttggaaac agatttttta ttactaaaat atgagcatta ttgacaccac
     1561 aaaagatagt ggggtcagca tgttccaaca cgattacttg ttctgtcatt ggtaaaattt
     1621 tggtatgcaa aggtacatat tttcttttat attttacagc aaatttatca ttacttaatt
     1681 ttttaagtaa tattaggata ggaaactttc tatcatcgag aaaaaagata gggatataaa
     1741 acaagtaatg tgaattcttt ctgttttaca ccataaaaat tttctaccaa tagcatcatt
     1801 tatcaaaaaa atagtatttt tgtagcacca aagcccatct ttagctatag taagtgtgca
     1861 aaaggagaca ttatttactt agtagtaatg agtcttatca accttcacgg ttgttaaggt
     1921 atgcttgata aggctttttt gtttttatag taaacaaata gtaaacactt gaatattaac
     1981 cttaaaatga ataaatgtat ggaaaagcct tacgtagtag gtattgatat tggcggaaca
     2041 aacacagttt ttggtatcgt agatgcacgt ggaagtgtat tggctactaa ttcgattaaa
     2101 acacaaaaac acactgtgtt agaagattat atacaggaat tgtatgatga gttaactaaa
     2161 ttaattgccg ataacaccca tatttctaaa attaagggca taggtattgg agctcccaat
     2221 gcaaattatt ttaatggtac tatcgaacaa gcccccaact taccatggaa aggtattatt
     2281 ccgtttgctg ataaaattca aaagaaattt ggcttacctg tagccatcac caacgatgcc
     2341 aatgcagcag ctataggtga aatgatttat ggagcagctc gtggtatgaa aaactttatt
     2401 atgattacct taggcacagg agtagggagt ggtatagtag ccaatggctc gcttgtgtat
     2461 ggtagcgatg ggtttgctgg tgaactagga cataccacta tgcgtcgcaa caacggacgt
     2521 ttgtgcggtt gtgggctaac aggatgccta gaaacctatg cttcggctac gggtgtggca
     2581 cgttcggctc gcgaattttt agaaacacaa aaagacgtac caagccgatt gagagatata
     2641 cctgctgatg aaattacttc caaagatgtg tacgaagctg ctgtagcagg agataaatta
     2701 gcacaagaag tatttgcctt tacagggcag attttaggcg aagcttttgc cgattttatt
     2761 aaattctcta gtcccgaagc cattattatg tttggcgggt tgtcaaaagc aggagagtta
     2821 ttattacgtc caatacgcga atcgatggag aaaaacgtaa tgccaatttt tagaaacaaa
     2881 gtaaaattat tgttctccga actcaaagaa gcagatgctg ccgtattagg tgccagtgct
     2941 ttagcctggg aaataaaaga ttaatacgca cgtttcaact atttacacag aaccataagc
     3001 ctaagcattt gcctaggctt attttgtata gttgttgaat attgctcggt tttggtgtga
     3061 gggggggctg ttgttattta aactttttgc aaatatatta aataagcacg acttctattt
     3121 aaaattctgc tgatattttt atttttttgc cttgcagcta acggcagtct gtcaaaaaac
     3181 ctccgtttag tttaattttt gctcaaatgt aatgggtttt aacgtacttt caaaatatac
     3241 tagtgattta tttttctacc attggtcaac tgt
//
LOCUS       DAOPUR010000137         3266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30035_length_3266_cov_3.115229, whole genome shotgun sequence.
ACCESSION   DAOPUR010000137 DAOPUR010000000
VERSION     DAOPUR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30035_length_3266_cov_3.115229"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PKN90_03260"
     CDS             complement(<1..1175)
                     /locus_tag="PKN90_03260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ85330.1"
                     /translation="MQQVAFLPKVSVVIPIYNMELYLEETILSVLASSYTDYEVLLID
                     DGSTDNSVSIAKRFANAYPHIYFYEQPNQGVSAARNNAIKWSKGDYILPVDADNLIGK
                     EYISEAVHVLETYPNVKVVTCEAEFIGEKSGKWKQLPFSLSLLARKNMIDNCAMYRKS
                     DWQDCGGYCEEILGREDWDFWISMLKNGGDVVRLPIVGLYYRVRSNSKRRKTQHRKKK
                     LIDLLNVRHADFFEKQLHGRLHYNRTYSKLFNLLEKIFGKRTTVIHPTYSQLAPCIER
                     LPLAFLVNNNVIHQGRNTLKQFSENGLDLVVKSYQIPHIINRLSYGFFRASKAKRAYE
                     YAIILQQQAIGTPQAIAYIEQRFAGLLYQSYFVSVRSTCPYTFNTLIQQPSYEYRTLV
                     "
     gene            complement(1175..1891)
                     /locus_tag="PKN90_03265"
     CDS             complement(1175..1891)
                     /locus_tag="PKN90_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ85331.1"
                     /translation="MQKIHVITPVKDSIELSLQTIQSVCNSQTSMPFVYTVYNDFSTE
                     ENTQTLQQASQEFGFELINLSSITSTPSPNYLLVLQLAQQKAIDEDAALVIVESDVLV
                     QTNTLQALIDTAQQKSDAGMVASITVDESGNVNYPYLFAKKIKGLVKPTNKHVSFCCT
                     LLSLPYLKAYSFTSLQPDKNWYDVHISRQSRQLGFVNYLLGNHPVVHRPHSSRPWKQL
                     KYSNPIKYYWLKITQQKDKI"
     gene            2121..3140
                     /gene="pheS"
                     /locus_tag="PKN90_03270"
     CDS             2121..3140
                     /gene="pheS"
                     /locus_tag="PKN90_03270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNZ85332.1"
                     /translation="MKEKIEKLLAEIDILSPISKEEVEELRIKYLSKKGEISTLFNDF
                     RNVANEEKKVVGQLLNDLKNKAQDKINELKETFDNQQNNTNDLDLTRSSSPIELGTRH
                     PLSIVKNEIVDIFARIGFTISEGPEIEDDWHVFSALNFPPEHPARDMQDTFFIEKNPD
                     VLLRTHTSSVQTRVMKQAQPPFRVLCPGRVFRNEAISYRAHCFFHQVEGFYVAENVSF
                     ADLKQALLYFAKEMFGEHTQIRLRPSYFPFTEPSAEMDISCNLCGGKGCPFCKHTGWV
                     EILGCGMIDPNVLESCGIDSKKYTGYAFGMGIERITNLKFQVKDLRMFSENDVRFLKQ
                     FEAAL"
BASE COUNT         1066 a          600 c          592 g         1008 t
ORIGIN      
        1 accaatgtac gatattcgta cgaaggttgc tgaattaatg tattgaaggt gtacggacag
       61 gtagaacgca cacttacaaa ataactctga tagagtaagc ctgcaaagcg ttgctcgatg
      121 tacgcaatcg cttgtggggt accaattgcc tgttgttgta agataatggc atattcgtag
      181 gctcgcttgg cttttgatgc acgaaaaaat ccatacgata atcgattgat tatatgtgga
      241 atttgatatg atttcacgac caaatccaag ccgttttcag aaaattgttt caaggtattt
      301 ctgccttgat ggataacatt attatttact aaaaacgcta atggcagtcg ttcaatacat
      361 ggagccagtt gcgaataggt aggatgtata acggttgttc tcttaccaaa gattttctcc
      421 aacaaattaa acaactttga ataggtgcga ttataatgta accgaccgtg caattgtttc
      481 tcaaaaaaat ccgcatgacg cacattcaac aaatcaatta atttcttttt gcggtgttgt
      541 gttttacggc gtttagaatt ggaacgcacc ctataatata accccacaat gggcaaacgc
      601 accacatctc ccccattttt gagcatcgaa atccaaaaat cccaatcttc ccttcccaaa
      661 atctcctcgc aataaccacc acaatcttgc caatcacttt tacgatacat ggcacagttg
      721 tcaatcatat tcttacgtgc aagcaatgac aagctaaagg gtaattgttt ccattttccc
      781 gatttttcgc ctataaactc agcttcgcac gttactactt ttacattggg gtaagtttct
      841 aatacatgta ccgcctctga aatatactct ttaccaatca gattatcagc atcaacgggc
      901 aaaatataat cgcctttgct ccacttaatc gcattatttc tagctgccga tactccttgg
      961 ttaggttgtt cgtaaaaata aatatgaggg tatgcgttgg caaaacgttt ggcaatagaa
     1021 accgaattat cagtagaacc atcgtctatt agtaacacct cataatctgt ataagatgac
     1081 gcaagtaccg acaagatggt ttcttctagg tatagttcca tgttgtaaat aggaattacg
     1141 accgaaactt taggtaaaaa ggctacttgt tgcatcaaat tttgtctttt tgttgggtaa
     1201 tttttagcca gtaatactta attggattcg aatatttcaa ttgtttccac gggcgactac
     1261 tatgtgggcg atgtaccaca ggatggtttc ccaataaata attaacaaaa cccaattggc
     1321 gtgattgacg agaaatatgc acatcatacc agtttttatc tggttgtaat gaggtaaagc
     1381 tataagcttt taaatagggt aacgacaaga gtgtacaaca aaaactcaca tgcttattgg
     1441 tcggttttac tagccctttt attttttttg caaacaagta tggataattt acatttccac
     1501 tttcgtccac ggtaatagaa gccaccattc cagcatccga tttttgttgt gcagtgtcta
     1561 ttaaagcttg tagtgtgttc gtttgcacca gcacatctga ctctacaata accaatgcgg
     1621 catcttcatc aatggctttt tgctgagcga gttgcaacac caacaaataa tttggagaag
     1681 gagtagaagt aattgacgat aaattaatca actcaaaacc aaactcttgc gatgcttgtt
     1741 gtaacgtttg tgtattttct tctgtgctaa aatcgttgta caccgtatat acaaaaggca
     1801 tagaagtttg cgaattacaa acagactgta tggtttgtaa acttaattca atcgaatctt
     1861 taacgggtgt aataacatga attttttgca tctggattac atcaaaatta tatatagaga
     1921 gaagcaaaag tgcttagtga tgcaaaagta catatttttt tatagaaaag taaaacagaa
     1981 tgactagaag agcaaaaaaa cagcaaaaag tcaaaaaaaa ccaaataaat aatgtatttt
     2041 tgtggttctt ttgtggtata tgccacttat attttaaaca aacatagtac tcattcgtta
     2101 tcgatattat tgatagactc atgaaagaaa aaattgaaaa actattagct gagattgaca
     2161 ttctaagccc tataagcaaa gaggaagtag aagaacttcg catcaaatac ttaagtaaaa
     2221 aaggagaaat aagtacttta ttcaatgatt ttagaaacgt tgccaacgaa gaaaaaaaag
     2281 tagtgggaca attactaaac gacctaaaaa ataaagcaca agataaaatc aatgagctta
     2341 aagaaacatt tgataaccaa caaaataaca ccaacgacct agacctaact cggagttcat
     2401 ctcctattga attaggtaca cgtcatcctt tatctatcgt taaaaacgaa atcgtagata
     2461 tttttgcacg tatcggtttt actatttctg agggtccaga aattgaagat gactggcatg
     2521 tattttctgc ccttaatttc ccccccgaac atcctgcacg cgatatgcaa gatacattct
     2581 ttatcgaaaa aaatcctgac gtattgttgc gtacgcatac ctcatcggta caaactcgtg
     2641 tgatgaaaca agcacaacca cctttcagag tattgtgtcc aggaagggta tttcgtaacg
     2701 aagccatttc gtaccgtgcc cattgtttct ttcatcaagt agaaggattt tatgtggctg
     2761 aaaatgtgtc ttttgctgat ttaaaacaag ctctattgta ttttgcgaaa gaaatgttcg
     2821 gcgaacacac acaaatccgt cttcgtccat cgtatttccc ttttaccgaa ccttcggctg
     2881 aaatggatat atcctgtaac ttatgcggag gcaaaggttg tccgttctgc aaacacactg
     2941 gttgggtaga aattttagga tgtggtatga tagacccaaa tgtactcgaa agctgtggta
     3001 ttgatagtaa aaaatacact ggatatgctt ttggtatggg tattgaacga attacgaacc
     3061 tcaaatttca agtgaaagat ttacggatgt tctccgaaaa tgacgtgcgt ttcctaaaac
     3121 aatttgaggc agctctataa gcattttttc tgacatatac ttttctcttt tggcagatat
     3181 ttgtattggc atacattttg attagtatag tattgtattg ctttagagac gagataaaac
     3241 atcaaaaata aaggctatgg gtagcc
//
LOCUS       DAOPUR010000138         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30160_length_3257_cov_3.837914, whole genome shotgun sequence.
ACCESSION   DAOPUR010000138 DAOPUR010000000
VERSION     DAOPUR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30160_length_3257_cov_3.837914"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PKN90_03275"
     CDS             complement(<1..563)
                     /locus_tag="PKN90_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85333.1"
                     /translation="MLYKFIFISDEVDNFMREITIDSAATFLDLHKAILESVSYTEEG
                     MASFFICNDYWEKEQEITLIEMDTSSEYDNYVMEQTGLEEFISEEKQKILYVFDYFTD
                     RAFFIELKEIIPTKRQEKALCTKKMGEAPSQFVADIFEEDKVTKPAKVVTPLDENFYG
                     DEDFDMDELDEESFSDVDFSEESDPLK"
     gene            complement(679..2049)
                     /gene="dnaA"
                     /locus_tag="PKN90_03280"
     CDS             complement(679..2049)
                     /gene="dnaA"
                     /locus_tag="PKN90_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNZ85334.1"
                     /translation="MNSLYTTFWDKCLNIIRDNVNETSFSTWFEPIIPIKYENNVFTI
                     QVPSQFFYEYIEAQYADLLKSTLNRVIAPNTKLVYRVVVDNNNKKNGTTDVPGAEKAP
                     FQGKKKIGVPEFDSYLNFNYHFDNFVEGDCNRLPRTAGINIASQPGKTIFNPLFIYGK
                     SGVGKTHLANAIGLKTKELHPQKKVLYVSANLFQLQYTEASRNNQINDFLNFYQLIDV
                     LIIDDIHELGEGKKVSTQNTFFHIFNHLHQSGKQLIITCDKKPAELEGLEDRLLTRFK
                     WGLSAEIEKPDFETRKAILLSKTERDGLKIPDNVLSFIAEHITDSIRDLEGAIISLIA
                     QSTMNQKVINLDLAKKVVRNLVNVTEKKHTVKNIRDLVCEYFKIPVESLQSSSRKREV
                     VQARHIAMYFSKQLTQESLTTIGNSIGKRNHATVIHACKTVKDLMDTDKTFRGNVLEI
                     EKKLTF"
     gene            complement(2338..>3257)
                     /locus_tag="PKN90_03285"
     CDS             complement(2338..>3257)
                     /locus_tag="PKN90_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNZ85335.1"
                     /translation="NKYTMKKFFTREAKIGLLVIVALGLLYFGINYLKGINLFKPTKY
                     FYVQYDRIDGVVKTSPVLINGYKIGHVSNIDFDYTKNAPITLEISVDKHLIVPKGSYA
                     ELYETGMLGDKAIQLVLTDNPDIHQIGDTLPGKTSGGLIGEIVNSLLPPIQKVIPQVD
                     STLLALRKVIESGAVENSMKNIETTTQELASMSQSLKKIVHTDVPVIMNDVSTVTQNL
                     SKVTTDLSKVDLEKTLISLNETLANLQLASSKFNEKSNTVGLLLNDPSLYLNLNATMD
                     SSNKLLIDLKENPKRYIHFSVFGAKKEKK"
BASE COUNT          972 a          523 c          522 g         1240 t
ORIGIN      
        1 cattttagag ggtcactttc ttctgaaaaa tcaacatcgc tgaaactttc ttcatcaagt
       61 tcatccatat caaaatcttc atcaccatag aaattttcgt caaggggagt aacgactttt
      121 gctggttttg ttactttgtc ttcttcaaat atgtcagcta caaactgcga tggagcttct
      181 cccatttttt ttgtacatag tgccttttct tgtcttttgg taggaattat ttcttttagt
      241 tcaataaaaa aagctctgtc tgtaaaataa tcaaacacat acaaaatttt ttgtttttct
      301 tctgaaataa attcttccaa acctgtttgt tccatcacat aattatcata ttcagagctg
      361 gtatccattt caataagcgt aatttcttgt tctttttccc agtaatcatt acaaataaag
      421 aaagaagcca tcccttcttc tgtgtacgat actgattcca aaatagcttt gtgtaaatct
      481 aagaaagtgg ctgccgaatc aattgtaatt tcacgcataa aattatccac ttcgtctgaa
      541 ataaaaataa atttgtatag catactagtt taattttgca tatatataat tataggcgta
      601 aaaatacagc tttttttctt tttgagaaac attgtcaata aaaaaagcga ccgttttggt
      661 cgctttttgc tgagatgctt aaaaagtcaa ttttttttct atttctaaca catttcctct
      721 aaaggttttg tctgtgtcca tgaggtcttt tactgttttg cacgcatgaa taactgtagc
      781 atgattgcgt ttgccaattg aattaccaat tgtggtgaga gattcttgag taagttgttt
      841 agaaaaatac atagcaatgt gtcgtgcttg taccacttca cgtttgcgtg agcttgattg
      901 cagactctca acaggtattt taaaatactc acataccaaa tcacgaatat tttttacagt
      961 gtgttttttt tctgtaacat tgactagatt acgcactact ttttttgcca aatctaagtt
     1021 tattactttt tgattcatgg ttgattgagc aataagcgaa ataatagctc cctctaaatc
     1081 acgaatactg tctgtaatat gttcggcaat aaatgaaagt acattatctg gtatttttaa
     1141 tccgtccctt tctgttttgc taagcaaaat tgcctttcta gtttcaaaat caggtttttc
     1201 tatttcagct gataatcccc atttgaaacg tgttagcaaa cggtcttcta atccttccaa
     1261 ttcggcaggc tttttgtcac aggtaataat aagttgtttg cctgattggt gtaagtggtt
     1321 gaatatatga aagaatgtgt tttgtgtact taccttttta ccttcgccta attcgtggat
     1381 atcgtcaatt atcaacacat caattagttg atagaaatta aggaaatcgt tgatttgatt
     1441 gtttctggat gcttcggtat attgtaattg aaataaatta gctgatacgt ataatacttt
     1501 cttttgaggg tgtaattctt ttgtttttaa tccaatagca ttggctaagt gtgttttgcc
     1561 tacacctgat ttgccataga taaatagcgg attaaaaata gttttacctg gttgacttgc
     1621 aatattaata cctgctgtac gtggcaaacg gttgcaatca ccttctacaa aattatcaaa
     1681 atggtaatta aagtttagat atgaatcaaa ttctggaacc ccaattttct tttttccttg
     1741 aaaaggagcc ttttcggcac caggtacatc tgtagttcca ttttttttat tgttgttgtc
     1801 tacgactact ctgtatacca gttttgtatt tggtgcaatg acacggttta gcgttgattt
     1861 taataaatca gcatactgtg cctctatgta ttcgtaaaaa aactggcttg ggacttggat
     1921 ggtaaatacg ttattttcgt atttgatggg tatgattggc tcaaaccatg tactaaagct
     1981 tgtttcgttc acattatctc gaataatgtt taagcatttg tcccaaaaag tagtgtaaag
     2041 agaattcata gtcgtttttt catgtaaaag atttatcgtt gggttgcaaa tttcataaaa
     2101 attattcaaa aaaaaaaatc attttaaact tgtttttttt aaatatttgt atctatttgt
     2161 ctttcagtta cttgttttaa tttattgata atcagatata tataatctaa aaaactatca
     2221 atttattgac agttaggctt ttaaaaaata tgttaattta tgagtcaaaa agatgaaact
     2281 cctttatagt aagaagttcc atctgaatgt taacttaatg ttaagattgt tgaaagctta
     2341 ttttttttct ttttttgccc caaaaacaga aaaatgaata tagcgtttcg gattttcttt
     2401 taaatcaatt agcaatttat tagaactatc catggttgca ttcaaattga gatacaacga
     2461 tgggtcgttc agcaaaagcc ctacagtatt gctcttttca ttaaattttg acgaggctaa
     2521 ttgcaaatta gctaatgttt cattaagcga aataagtgtt ttctctaagt caaccttgct
     2581 caaatcggtt gttacttttg ataaattttg tgtaacggta ctcacatcgt tcataattac
     2641 gggaacatcc gtatgtacta tctttttgag cgattgcgac atggatgcaa gttcttgtgt
     2701 ggtagtttca atatttttca tgctattttc tactgcacca ctttcaatta ctttgcgaag
     2761 agccaataat gtagaatcaa cttgtgggat gactttttgg ataggaggta ataacgaatt
     2821 aacaatttca ccaattaatc ctccagatgt tttaccaggt agggtgtcgc caatttggtg
     2881 tatatcagga ttgtctgtca aaacgagttg tatagcttta tcgcccaaca tacctgtttc
     2941 atagagctca gcatacgaac ctttgggcac tattaggtgt ttatcaacag atatttcaag
     3001 ggtaattgga gcattttttg tgtaatcaaa atcaatattg cttacatgtc caattttata
     3061 tccgttaatc aaaacgggac tcgtttttac tacgccatct attcggtcgt attgaacgta
     3121 aaaatatttt gttggtttaa atagatttat tccttttagg taattaatac caaaatacag
     3181 cagacctagt gctacaatta ctaataatcc aattttagct tctcgtgtaa agaatttttt
     3241 catagtatat ttattat
//
LOCUS       DAOPUR010000139         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30165_length_3257_cov_3.107745, whole genome shotgun sequence.
ACCESSION   DAOPUR010000139 DAOPUR010000000
VERSION     DAOPUR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30165_length_3257_cov_3.107745"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="PKN90_03290"
     CDS             complement(<1..111)
                     /locus_tag="PKN90_03290"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNZ85336.1"
                     /translation="MSTKQKTLQQPFTLEGKGLHTGLHIKATFMPAPDNTG"
     gene            complement(113..1156)
                     /gene="lpxD"
                     /locus_tag="PKN90_03295"
     CDS             complement(113..1156)
                     /gene="lpxD"
                     /locus_tag="PKN90_03295"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNZ85337.1"
                     /translation="MELSAQQIAAYLHGEVVGDASVTVNTFAKIEEGKPHSLSFLANP
                     KYTHYIYETQASIVLVNKTFIPEKPINATLIKVDDAYKSLADLLTLVENNKPKKTGID
                     PLAYIAPSATIGKNVYIGAFVYIEEGVVIGDNCQVYPHTYIGEKSTIGKDSILYSGVK
                     IYHQCVIGNQCIIHASSVIGADGFGFARNEDGSYSKIPQSGNVVVEDNVEIGACTTID
                     RASMGSTFIRKGVKLDNQIQIAHNVELGENTAMAACSGIAGSTKVGKNCILAGMVGLA
                     GHLNVPDNTIFAAYTGVPNNIKEAGIYQGIPAIPVAQFRRASVVYKQLPELQKTIIAL
                     QKEIELLKAELKK"
     gene            complement(1256..2479)
                     /locus_tag="PKN90_03300"
     CDS             complement(1256..2479)
                     /locus_tag="PKN90_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ85338.1"
                     /translation="MSNNKKKIINDPVFGFINIPGTLLYNLLQHPYLQRLTRIKQLGL
                     SSYVYPGAQHTRFQHSLGAMYLMTEAIKTLKSKGISISQHETEAACAAILLHDVGHGP
                     FSHALEGMIIPHLSHEEITLRIMQKINKEYEGKLDLALEIFQNTYHKTFLHQLVSGQL
                     DMDRLDYLRRDSFFTGVTEGNIGSARIIKMLNIKNDTLVVEAKGIYSIENFLMNRRLM
                     YWQVYFHKTAFAIEKMLIKTIERAKIIAKQDSTLFGTPSLLFFLRQEDNLTNDSALDL
                     FLSLDDTDIMCSIKQWINHPDTILSTLSYGIIHRKLFKIEVSNEPFSKPKIEAKTTEI
                     AQHLGINHMDATYFVSVNDVSINMYHNNEDSITILHKDGSIKPISQTSDMLDIRILSR
                     EVTKYYFSYYKITPE"
     gene            complement(2484..>3257)
                     /gene="pyrF"
                     /locus_tag="PKN90_03305"
     CDS             complement(2484..>3257)
                     /gene="pyrF"
                     /locus_tag="PKN90_03305"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNZ85339.1"
                     /translation="DTDITKIPEVLFDSCDDPMFVFNKRIIDATADLCVAYKPNLAFY
                     ESLGLEGWDVLERTIDYIRTNYPDQFIIADAKRGDIGNTSAMYARTFFGTMDFDAVTV
                     APYMGEDSVTPFLTYENKWVILLALTSNKGAFDFQFLQDKETNERLFEKVLHTSAQWG
                     NTDNMMYVVGATKAEMLQDIRAIIPNHFLLVPGVGAQGGSLQEVAKYGMNAECGLLVN
                     SSRQILYASSGEDFAEAARLEALAVKQEMEQLLKDAHLV"
BASE COUNT          932 a          611 c          593 g         1121 t
ORIGIN      
        1 tccggtatta tccggagctg gcataaaagt agctttgatg tgtaagccgg tgtgtaaccc
       61 ttttccttct aaagtaaatg gttgctgtaa cgttttttgt tttgtcgaca tcttactttt
      121 ttaattctgc ttttagtaat tctatttctt tttgtaaggc aataatagtt ttttgtagtt
      181 caggtaattg tttatatacg actgaagcac gtctaaattg tgctacaggt atggctggta
      241 tgccttggta aatgcctgct tctttaatat tatttggcac accagtataa gccgcaaaaa
      301 tggtgttatc gggcacattc aagtgtccag ctaacccaac cataccggct aaaatgcaat
      361 tttttcccac tttggtagag cctgcaatgc ccgaacatgc tgccatagca gtattttcgc
      421 ccaactctac attatgggct atttgtattt gattgtctaa tttaacgcct ttacggataa
      481 atgttgagcc catactagct cggtcaatgg tagtgcatgc accaatttct acattatctt
      541 ccactactac attgccactt tgtggtattt tcgagtagct tccgtcttcg ttgcgagcaa
      601 agccaaagcc atctgcacca attaccgaag aggcatgaat tatgcattga ttaccaataa
      661 cacattgatg gtaaattttt acgcctgaat ataaaatgct atctttccca atagtgcttt
      721 tttcgccaat gtaggtatga ggatatactt ggcaattatc gccaataacc actccttctt
      781 ctatgtatac gaatgctcca atatacacgt tttttccaat agttgctgaa ggtgcaatgt
      841 atgctaaagg atcaatacct gttttttttg gtttattgtt ttcgactaag gtaagtaaat
      901 ctgctagtga tttgtaggca tcatctactt ttataagagt cgcattgatt ggtttttctg
      961 gtataaatgt tttatttacc aacacaatac ttgcttgggt ttcgtaaata tagtgcgtat
     1021 atttagggtt agctaaaaaa ctcaacgaat gaggttttcc ttcttcaatt tttgcaaaag
     1081 tatttactgt aacggaagcg tcaccaacga cttccccatg tagatatgca gctatttgct
     1141 gagcggacaa ttccatatag gcacaaattt tggcaaaggt acatattttt tacaaatgaa
     1201 gagctaattt tttatcagaa agtagtaaca ttttacaaca ttttgtttat tttttctatt
     1261 caggtgttat tttgtaataa ctaaaatagt atttcgttac ttctcgagat aaaatgcgta
     1321 tatccaacat atccgaagtt tgacttatgg gtttaatact accgtctttg tgtaaaatag
     1381 taatactatc ttcgttgttg tgatacatat taattgatac atcatttacc gacacaaaat
     1441 aagtagcatc catatggttt atacctagat gttgtgctat ttctgttgtt tttgcctcta
     1501 ttttcggttt gctaaatggc tcgttactaa cttctatttt aaataatttt cggtgaataa
     1561 tgccatagct gagagtagat aaaatcgtat cagggtggtt tatccattgc tttatagagc
     1621 acataatgtc ggtatcgtcg aggcttaaaa ataaatctaa cgcactatcg ttggtcaaat
     1681 tatcctcttg acgtaaaaaa aagagcaacg atggggtgcc gaatagcgtg ctgtcttgct
     1741 tggcaataat tttcgctctt tcgatggttt tgataagcat tttttcgatt gcgaaagctg
     1801 ttttatgaaa atatacttgc caatacatca ggcgtctgtt catcagaaaa ttttcgatag
     1861 aataaattcc tttggcttct actactagcg tatcgttttt tatgtttaac attttgataa
     1921 tacgtgccga accaatattg ccttcggtaa ctcccgtaaa aaaactatct ctacgcaaat
     1981 aatcgagcct atccatatcc aattgacccg aaactaactg atgcaaaaat gttttgtgat
     2041 aggtgttttg aaagatttct aaggctaaat caagttttcc ttcgtattct ttattgattt
     2101 tttgcatgat acgcaaggtt atttcttcgt gacttaggtg gggaatgatc ataccttcta
     2161 aggcgtgaga aaaaggacca tgccccacat catgcaataa tatggcggca caagcagctt
     2221 ctgtttcgtg ttgcgaaatg ctaattcctt ttgatttgag tgttttaatt gcttctgtca
     2281 tcaagtacat agcccccaag gagtgttgaa agcgtgtgtg ctgtgcccca ggatatacat
     2341 acgatgataa accaagttgt ttgatgcgtg taagtcgttg caaataagga tgttgcaata
     2401 aattatacaa caatgtgcct ggtatgttga taaaaccaaa aacagggtcg ttaattattt
     2461 tctttttgtt gttagacacg gcattacact aaatgagcat ctttcaataa ttgttccatt
     2521 tcttgtttta cagcaagggc ttcgaggcga gcagcttctg caaaatcttc tcccgaagag
     2581 gcatatagta tttgtcgcga cgaatttact aacagaccac attcagcatt cataccgtat
     2641 tttgccactt cttgcaaact gccaccttgt gctccaacac ctggaacaag caaaaaatga
     2701 ttcggaataa tagcacgaat atcctgtaac atttcggctt ttgtagcacc tactacatac
     2761 atcatattat cggtatttcc ccattgagca gatgtgtgga gtactttttc gaacaagcgt
     2821 tcattggttt ctttgtcttg taaaaattga aaatcaaaag ctcctttgtt cgatgttaac
     2881 gccaacaaaa tgacccactt attttcgtat gttaaaaaag gtgttacgct gtcttcgccc
     2941 atataaggag ctactgtaac tgcatcaaaa tccattgtgc cgaagaatgt acgtgcatac
     3001 atagccgaag tgttgcctat atcgccccgt ttagcatcag caataataaa ttggtcaggg
     3061 taatttgtac gaatataatc aatggtacgc tctagcacat cccaaccttc taatcccaaa
     3121 ctttcgtaaa aagctaagtt gggtttgtat gcaacgcata aatcagccgt agcatcaata
     3181 attcgtttgt taaacacaaa catggggtcg tcgcaactgt caaaaagtac ttctggtatt
     3241 ttagtaatat cggtatc
//
LOCUS       DAOPUR010000140         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30242_length_3250_cov_3.231299, whole genome shotgun sequence.
ACCESSION   DAOPUR010000140 DAOPUR010000000
VERSION     DAOPUR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30242_length_3250_cov_3.231299"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..492
                     /locus_tag="PKN90_03310"
     CDS             118..492
                     /locus_tag="PKN90_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNZ85340.1"
                     /translation="MTTRLLILSILIFTITSCTSQTSSFKSVDAKEFEQILRTDTLQI
                     VDVRTPEEFNQGHIQGAICMDVRNPDFDSKIIQLDKHIPVAIYCRAGRRSKIAAEKMS
                     AIGLQGYELDNGIVSWENKLTR"
     gene            683..1912
                     /gene="mtaB"
                     /locus_tag="PKN90_03315"
     CDS             683..1912
                     /gene="mtaB"
                     /locus_tag="PKN90_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNZ85341.1"
                     /translation="MIDTSVFDNKTVAFYTLGCKLNFSETSAIGKSLIEQGYRKVAPH
                     EPASLCIINTCSVTDSADKKCRQTITKAIKENPGAFVIVTGCYAQLKPDDITSIPNVD
                     LVLGANDKFNIPDIIAKKTAMPNKLQASPTNRMTEFHHSYSSEDRTRHFLKVQDGCDY
                     FCSYCTIPLARGRSRNGSIENLVKDAQSIAALGGKEIVLTGVNIGDFGHSTNESFIDL
                     LKALEQVEGIERYRISSIEPNLLTDDIIALVAQSTKLMPHFHIPLQSGCNEVLNLMKR
                     RYTTELFAQKIATIKQLMPHAFIGVDVIVGVRGETTSYFETTYQFIESLPISQLHVFS
                     YSERAGTQALKIEHVVTPNEKKRRSHLLLSLSEQKTEAFYNNFKHSIRPVLWEGTKKG
                     GFMYGFTDNYIRVKRLT"
     gene            1985..3115
                     /gene="tgt"
                     /locus_tag="PKN90_03320"
     CDS             1985..3115
                     /gene="tgt"
                     /locus_tag="PKN90_03320"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNZ85342.1"
                     /translation="MTFELQHTDPKSNARAGLITTDHGQIETPIFMPVGTVASVKAVH
                     QHELKEDIKAQIILGNTYHLYLRPGIEILEKAGGLHKFNGWDKPILTDSGGFQVFSLS
                     HRRKMKEEGVTFHSHIDGSKHLFTPENVVDIQRIIGADIIMALDECTPGDADYAYAKK
                     SMELTHRWLARGIQHFDATEPKYGYSQTFFPIVQGCVYPDLRKQSAEFVASQGREGNA
                     IGGLAVGEPTEKMYEMIEIVNEILPTDKPRYLMGVGTPVNLLEAIERGVDMFDCVMPT
                     RNGRNGMLFTKNGIINMRNKKWADDFSPIEIDGASYVDTAYSKAYLRHLFISNELLGL
                     QIASIHNLAFYLWLVQEAHKQILAGTFSVWKTNMIQQVSQRL"
BASE COUNT         1154 a          567 c          593 g          936 t
ORIGIN      
        1 attgtcattg cttcatatgg aaaattcgga gggtctaatt caaaaaaaaa tatcattcca
       61 agtttattaa ttatgtgtac atttgtggta attatgcata cagtaacata aaaaaatatg
      121 acaacccgtt tacttatttt aagtatactt attttcacca tcacaagttg caccagtcaa
      181 acaagttctt ttaaatcagt tgatgcaaaa gagtttgagc aaatattacg tacagatact
      241 cttcaaattg tggatgtacg cacacctgaa gaatttaatc aaggacatat tcaaggggct
      301 atttgcatgg atgttagaaa tccagatttt gactcaaaaa taatacaatt ggataaacat
      361 atccctgtgg ctatctattg cagagctggg agaagaagta aaattgctgc tgaaaaaatg
      421 agtgcaattg gactacaagg atatgagcta gacaacggca ttgtgtcttg ggaaaataaa
      481 ttaactcgtt aactattttg taaaaggaca ataaaacgaa tcatccctat actgataata
      541 cctctatctg gtatatatcc acctaaaaaa catagctata aaacgttaag gaatattcat
      601 ttataacaac aaatagatgc aatacagcaa aaaaatagta cttttgcatc ctgattttga
      661 atgattccat gtacatataa ttatgattga cacatctgtt tttgacaata aaacggtagc
      721 tttttataca ctaggttgca aactgaattt ttcggaaaca tctgctatag gtaaaagttt
      781 gattgagcaa gggtatcgca aagtagcccc tcacgaacca gcctcgcttt gtattattaa
      841 cacctgctcg gtaacagata gtgctgataa gaaatgtaga caaactatta ccaaagccat
      901 taaagaaaac ccaggagctt ttgttattgt tacaggttgt tatgctcaac tcaaacccga
      961 tgatattact tctataccca atgttgattt ggtgttggga gcaaatgata aattcaatat
     1021 tcctgatatt atagcaaaga agactgctat gcccaacaaa ttgcaagcta gccctaccaa
     1081 ccgaatgact gaatttcatc attcatattc gagcgaagac aggacaaggc attttttaaa
     1141 agtacaagat gggtgtgatt atttttgtag ctactgtact attccattgg ctagaggcag
     1201 aagtagaaat ggctctatcg aaaatttagt aaaagatgca caaagcattg cagcacttgg
     1261 tggtaaagaa atagtattaa cgggcgtcaa tattggtgat tttggacatt caacaaacga
     1321 atcatttatt gatttattaa aggcacttga acaagtagaa ggaatagaac gttataggat
     1381 atcttctatt gaaccaaatt tattaacaga tgatattatt gctctagtgg cacaatctac
     1441 caaactaatg cctcattttc atattccact acagtcgggc tgtaatgagg tattaaactt
     1501 aatgaaaaga cgctacacaa ccgaattgtt tgctcaaaaa attgccacta taaaacaact
     1561 gatgccacac gcatttattg gtgtagatgt aattgtaggc gtacgaggag aaaccacatc
     1621 ctattttgaa acaacttacc aattcattga atctttacct atttcacaat tgcatgtatt
     1681 ttcgtattcg gaaagagcag gcacacaagc attaaaaatt gagcatgtgg taacacccaa
     1741 cgaaaaaaaa agacggagcc acttattact ctcgttatcc gagcaaaaga cagaggcttt
     1801 ttacaataac tttaagcatt ccatccgacc cgttttatgg gaaggcacta aaaaaggcgg
     1861 ttttatgtat ggatttacag ataactatat tcgtgtaaaa cgccttacgt aatacataaa
     1921 attaatacga ttgagtctat tatattaacc gcagaaacga ttagcataga aaaaagttaa
     1981 tgaaatgaca tttgaactac aacataccga ccccaaaagt aatgctcgag caggcttaat
     2041 aacaaccgac catggacaaa tagaaacacc catttttatg cccgtaggta cagtagcatc
     2101 agtaaaagca gtacatcaac acgagttgaa agaggatata aaagcacaaa tcatcttagg
     2161 aaacacctac catttatacc tccgaccagg tatcgagatt ctggaaaaag caggaggctt
     2221 acataaattt aacggatggg acaagcctat acttacggat agtggtggtt ttcaggtatt
     2281 ttcactttcg catagacgaa aaatgaaaga agagggcgta acctttcatt cgcatattga
     2341 tggttcaaaa catttgttta cacccgagaa tgtggtagat attcagcgta ttatcggagc
     2401 agacattatt atggctcttg acgaatgtac cccaggtgat gccgattatg cgtatgcaaa
     2461 aaaatcaatg gaacttacgc atcgatggtt ggcacgtggc atacaacatt ttgatgctac
     2521 tgaaccgaaa tatgggtata gccaaacatt ttttcccatt gtgcaaggat gcgtatatcc
     2581 agatttacgc aaacagtcgg cagaatttgt agcctcgcaa ggcagagaag gaaatgctat
     2641 tggaggcttg gcagtaggcg aacctaccga aaaaatgtat gaaatgattg aaatagtaaa
     2701 cgagatactg cctacagata aacctcgtta cctaatgggt gttggtacac ctgtcaacct
     2761 attggaagct atcgaacgag gggtagatat gtttgactgt gtaatgccta cacgcaatgg
     2821 cagaaacggt atgcttttta caaaaaatgg aattataaat atgcgtaata aaaaatgggc
     2881 agacgatttt tctcctatcg aaatagatgg agcatcgtat gtagataccg cctactctaa
     2941 agcctatttg cgtcatttgt ttatatccaa cgaattgtta ggattgcaaa ttgcatccat
     3001 tcacaatcta gctttttatt tatggttagt gcaagaggca cacaaacaaa tacttgcagg
     3061 cactttttca gtatggaaaa caaacatgat acaacaggta tcacaacgtt tataaaatat
     3121 ttttgttatt ttaaagacta aatagtactt ttgacactat aaaaagagta tattttcaga
     3181 ggattaaaca cacagatacc aagctatctt taacattgat caataatacc tatttattgc
     3241 atgaaaacac
//
LOCUS       DAOPUR010000141         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30244_length_3250_cov_2.993427, whole genome shotgun sequence.
ACCESSION   DAOPUR010000141 DAOPUR010000000
VERSION     DAOPUR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30244_length_3250_cov_2.993427"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKN90_03325"
     CDS             complement(<1..347)
                     /locus_tag="PKN90_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ85343.1"
                     /translation="MNNITTTIIEKMIQYFGTDVRRINHALKVYGFARCIAQKEKLTE
                     NELFIVDIVAILHDIGIIEAEKKYNSSNGKYQEIEGPAIAKNMLSDQHIDTTTIDRIC
                     FIIGNHHSYQKID"
     gene            complement(625..1467)
                     /locus_tag="PKN90_03330"
     CDS             complement(625..1467)
                     /locus_tag="PKN90_03330"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNZ85344.1"
                     /translation="MRKILLLFILTFLFVSCNQKNAEEVSYATTDLEEEMSPIAQQST
                     NESSESKETIEAKKINKKKIIKDGKLGIRVSELEITKLRIDSLIKKYGGYYANESFNN
                     SDWESSFTLKIRIPSVSFENFISDIEQGNGEILYKEIDARDVTDQFIDLETRLENKRN
                     YLIRYNELLKKANSVKEIVEIEEKIRGLEEEIESTVGRLKYLNDLVDYSTLDLTISKQ
                     KDFKYNPTKRDKFSEKLKQSVSRGWFGFVDFILFLIKIWPFWVIFPFIFYFWKRHKSK
                     KIKN"
     gene            1893..2504
                     /locus_tag="PKN90_03335"
     CDS             1893..2504
                     /locus_tag="PKN90_03335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ85345.1"
                     /translation="MEDTFAHKALTWDNPRKISMTNKFVKEIEKLCSFSSSLKILEIG
                     TGTGLVGLQLVSKASEMLLIDTSPSMLQILEKKLQDNPYPHVKCLHATIETINATDFD
                     LVISFMTWHHIADFSSAFTSVYTLLKKGGKLLIGDLLTEDGSFHGDEFVPHLGFDMQM
                     LRTIAENHGFKVLQVYPYAEVEKQVQNGDINRYGQFVLFAEKI"
     gene            complement(2583..>3250)
                     /locus_tag="PKN90_03340"
     CDS             complement(2583..>3250)
                     /locus_tag="PKN90_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNZ85346.1"
                     /translation="RDIFKTHIEIEKKGLFECDLSKNNLLINKDKQIMFFDFGYMYPY
                     NPLIHYNSDGKQLPIFHLCERLESRSLMQYLMDIENDSSLMIETFENTKRLALEAYSE
                     KLIWLEKNNADTDVIQWQKNWINQWEYSLKSPANFLETYELESFRSYVLDVHDDIGGK
                     SCTPMTIKKLDKILEQIKHNYPTLKIRNGLFWGDEKLNNSSLYDKYTKLKEQACRYQL
                     HET"
BASE COUNT          979 a          494 c          508 g         1269 t
ORIGIN      
        1 ttgtctattt tctgatagga atgatgattg ccaataatga aacaaattct atcaatagtt
       61 gtagtatcta tatgttggtc tgacaacata tttttcgcga tggcaggtcc ttctatttct
      121 tggtatttgc cgttactgga attatatttt ttttcagctt caatgatacc tatatcgtgt
      181 agtatagcca ctatatccac aatgaatagt tcattttctg ttagtttttc tttttgtgca
      241 atacacctgg caaagccata tactttaaga gcatgattta ttcttcgaac gtctgttcca
      301 aaatattgaa tcattttttc gataatagta gtagttatat tattcatatt tggtatgtta
      361 acgattgttg ttacagtatg atgtgtgtgt ctactaaaaa tggtttttct gtatctgttt
      421 gcttatgatg gtgataagcc tttgtttatt aacttagatt ctctattgaa taaattactt
      481 attttgaaca gaaattctcc ctgttagatt aaaattagca gggagaattt agtctagttg
      541 aatgaaatgt aggacatacg ttacatttgt aagtttaaaa ttatcagaat aacgtcgaga
      601 tatcgccttc ttagttgtat gtgattaatt ttttattttt ttgctcttat gtcttttcca
      661 aaagtaaaat ataaagggaa aaataaccca gaatggccaa attttgatca aaaaaagtat
      721 aaaatctaca aatccaaacc aacctctaga tactgattgc tttaattttt cagaaaactt
      781 atctcgtttt gttgggttat atttaaaatc cttttgtttt gatattgtta aatctaaggt
      841 gctgtagtca actaagtcgt ttaagtattt taatctccct actgtgcttt caatttcttc
      901 ttctaaccct cgtatttttt cttcaatttc tactatttcc ttaacgctat tggctttttt
      961 taatagctca ttatagcgta ttaggtaatt tcgtttattt tcaagtcttg tttctaaatc
     1021 aataaattgg tcagttacat cccgagcatc aatctctttg taaagtattt ctccattacc
     1081 ttgttcaata tcagatataa agttctcaaa agaaacactt ggtattctta ttttaagggt
     1141 aaaagacgat tcccagtcag aattattgaa actttcgtta gcataatacc cgccatattt
     1201 ttttattaaa gagtctattc tcaatttcgt gatttctaat tctgaaaccc taatgcctaa
     1261 ttttccatct ttgataattt ttttcttgtt tatttttttt gcttcgatag tttctttcga
     1321 ctcggatgat tcgttggtag attgttgtgc tatcggactc atttcttctt ctaaatcagt
     1381 tgttgcatat gaaacttctt ctgcattttt ctgattacaa gaaacaaaca aaaaggttag
     1441 aatgaataat aatagaatct ttctcatttt atagtaattt agttggttgt tgaaatttac
     1501 tactatgtac attacagtaa tgtgtttttt taatgctgct caaaagtaag atttttttaa
     1561 ggatagtaca aaatagaggg aaagaatttt ttagttagag tgttgcactt atacatatag
     1621 cattgcttag tctgcattct gtcgttgttt tacatggtat ttgtaaaaga ctaggataag
     1681 ttaaaaatat aaaaaagata atcgacagtg aacatagccg ttgttattga gcaaaaaatc
     1741 caattaaatt cattttcttt tgattatttc gtattttata gcgaaatttg tgaaggatga
     1801 taatgcaggt gtgtcttatt tcaaacatgg tcttttaaag ctctatcctc cattatttta
     1861 tgcttacaca ggacaatact atacctaatt ttatggaaga tacatttgcc cataaggctt
     1921 taacttggga taatccccga aaaatcagta tgaccaacaa atttgtgaag gaaatagaaa
     1981 aattgtgctc tttctcttct tccctcaaaa tacttgaaat tggaacaggt acgggtttgg
     2041 ttggtttgca gttagtatcc aaagcttcag aaatgttgct tatagataca tcgccatcaa
     2101 tgctacaaat tctagaaaaa aaattacaag acaatccata tcctcacgtt aagtgtctac
     2161 atgctactat cgaaacgatt aatgctacag attttgattt ggttatttct tttatgactt
     2221 ggcatcatat agcagatttt tctagtgcat ttacgtctgt gtatacactt ttaaaaaaag
     2281 gagggaaact ccttataggc gatttgctca cagaagatgg gagttttcat ggtgatgaat
     2341 ttgttcctca tttagggttt gatatgcaaa tgttgcgtac tattgccgaa aatcatggat
     2401 ttaaggtgtt acaggtatat ccgtatgccg aggtcgaaaa acaagtacaa aacggggata
     2461 tcaatcgtta cggtcaattt gtgttatttg ctgaaaagat ataggcatca atagtcgttt
     2521 agataccaac ttgttgtctg ttatagctgg aaacagggca tcaggaagta gatcttatgt
     2581 gattatgttt catgtagttg atatctacat gcttgctctt ttaattttgt atatttgtca
     2641 tataatgaag aattgtttag tttttcatct ccccaaaaca gcccatttct tatttttaat
     2701 gtaggataat tatgtttaat ttgttccagt attttatcta attttttaat tgtcattggt
     2761 gtacaactct tgccgccaat atcatcatgt acatctaata cataagaacg aaatgattcc
     2821 aattcgtacg tttcgagaaa gttagctggt gatttcaaac tatattccca ctgattaatc
     2881 caattttttt gccattgaat cacatctgta tcggcattgt ttttttctaa ccaaattaat
     2941 ttctcgctat acgcttctag tgctagtctt ttagtgtttt caaaggtttc aatcatcagg
     3001 ctcgaatcat tttctatatc cattaaatat tgcattaatg acctgctttc caatcgttcg
     3061 cataaatgga atataggtag ttgttttccg tcagagttgt aatgtataag tggattatac
     3121 ggatacatat agccaaaatc aaaaaacata atctgtttat ctttattaat taacagatta
     3181 tttttagaca aatcgcattc aaacagcccc tttttttcta tttcaatatg agttttaaaa
     3241 atatcacgta
//
LOCUS       DAOPUR010000142         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30364_length_3241_cov_2.697112, whole genome shotgun sequence.
ACCESSION   DAOPUR010000142 DAOPUR010000000
VERSION     DAOPUR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30364_length_3241_cov_2.697112"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /gene="mnmE"
                     /locus_tag="PKN90_03345"
     CDS             complement(<1..1278)
                     /gene="mnmE"
                     /locus_tag="PKN90_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNZ85347.1"
                     /translation="MNPTICAISSGQHQAAIALIRISGEEAFRMCDTFFVAANKAVTL
                     STMQPNSIKFGRIFQQNELIDEVLVSVFKAPHSFTGEDMVEIACHGSSFIQQQILQLL
                     IQQGCQLAQPGEFTRRAFLNGKMDLSQAEAVADLIAAQSASSHRVAMNQMRGGFSKEL
                     AVLRDKLLEFTALIELELDFSEEDVEFADRSSLMALATIIQTKLASLARSFEVGNVIK
                     NGIPVAIVGETNAGKSTLLNLLLNEEKAIVSDIHGTTRDVIEDIVSIQGITYRFIDTA
                     GIRETQDAIEKMGIKRTYQQIEQASIILWVIDSTQVNEHIDWMAERIVSRSAGKKLIL
                     VFNKLDKINEEECRVLELLFENITAERLFISAAKHQHIDALLHHLHQAAAIPSIGDQD
                     VIITNMRHFQALTQALSAIERVQTGLSTGLSGDF"
     gene            complement(1324..1452)
                     /locus_tag="PKN90_03350"
     CDS             complement(1324..1452)
                     /locus_tag="PKN90_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85348.1"
                     /translation="MKKIITSIAIAIILLLSVSSCQTKHCLSQVQEVLVELPTVNS"
     gene            1602..2018
                     /gene="mscL"
                     /locus_tag="PKN90_03355"
     CDS             1602..2018
                     /gene="mscL"
                     /locus_tag="PKN90_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNZ85349.1"
                     /translation="MGLLKEFKAFAMKGNVIDLAVAVIIGGAFGKIVSSLVADVIMPP
                     IGLLVGGVNFTELSIILKEATETTAAVTLNYGNFLQATFDFIIIAFAIFMFIKGLSKL
                     EKKKEEPVVPAAPVEPTPSKEEILLAEIRDLLKEKK"
     gene            complement(2077..2214)
                     /locus_tag="PKN90_03360"
     CDS             complement(2077..2214)
                     /locus_tag="PKN90_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85350.1"
                     /translation="MKKNLIKVVCVCAFLAMLFNSCKTPCHCGLGQVEADKATVETVQ
                     S"
     gene            complement(2237..2716)
                     /gene="ispF"
                     /locus_tag="PKN90_03365"
     CDS             complement(2237..2716)
                     /gene="ispF"
                     /locus_tag="PKN90_03365"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNZ85351.1"
                     /translation="MKIRVGFGYDVHQFAPNRALWLGGVSIEHSLGLLGHSDADVLIH
                     AICDALLGAANLRDIGYHFSDTSPEFKNIDSKILLAKTMNLLREQGYELSNIDATVCA
                     EEPKINPHIANMKETLAQVMHVSEEDISIKATTSEKMGFVGRKEGMAAYATVLIYKQ"
     gene            complement(2743..>3241)
                     /locus_tag="PKN90_03370"
     CDS             complement(2743..>3241)
                     /locus_tag="PKN90_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNZ85352.1"
                     /translation="GGSLTYPLDPYNKLSVNLDLNKLLVPSRPVAPTQGDDESDADYA
                     LRESKYETSLSDYNNGDPIAGIFTSFSDAPGGFSEELQEIMWSLGLEYAYNNQFFARA
                     GYFNESANKGNRKYATIGAGFKLNVFSLDVAYVVSVAQNNPLDQTLRFSLAFTLDGIK
                     QLTAR"
BASE COUNT         1001 a          622 c          569 g         1049 t
ORIGIN      
        1 aaaatcgccc gatagacctg tagataaacc tgtttgcaca cgttcaatgg cacttaatgc
       61 ttgtgttaat gcctgaaaat gacgcatatt agtaataata acatcttgat cgccaatact
      121 aggaatagct gctgcttggt gcaaatggtg taacaaggca tcaatatgtt ggtgtttagc
      181 cgctgatata aataagcgtt cagctgtgat attttcaaat aacaactcca acacacggca
      241 ttcttcttcg ttaatcttat ccaatttatt aaatactaag attaattttt tacctgcact
      301 tcgtgatact atgcgttctg ccatccaatc gatgtgctca tttacttggg ttgagtctat
      361 tacccacaaa atgatggatg cttgttctat ttgctgatag gtacgtttaa tacccatttt
      421 ttcgatagca tcttgggttt cgcgtatgcc agctgtatct ataaaacggt atgttatgcc
      481 ttgaatactt actatatctt caatcacatc gcgcgtagta ccgtgtatat ccgatacaat
      541 cgctttttct tcgttgagta ataagttaag caaggtcgat tttccagcat ttgtttcgcc
      601 tacaatcgct accggaatgc catttttaat cacgttccca acctcaaacg aacgtgccaa
      661 actggcaagt tttgtttgta taatagttgc taacgccatc aagcttgacc tgtcggcaaa
      721 ctccacatct tcttcgctaa aatctaattc aagctctatc aatgcagtaa attcgagtaa
      781 tttatctctc aacactgcca attctttaga aaacccaccc cgcatttgat tcatagctac
      841 tcggtgagaa gaggctgatt gtgcggcaat taaatcggct acggcttctg cctgacttaa
      901 atccattttg ccatttaaga aagctcttcg cgtaaactct cctggctggg ctaattggca
      961 accttgttgt attagtagtt gtaagatttg ttgttgaata aacgacgagc cgtggcaagc
     1021 tatctctacc atatcttcac ccgtaaacga atgtggagct ttaaatacac ttactaatac
     1081 ctcatctatc aattcgtttt gctgaaaaat tcgtccaaat ttgatactat ttggctgcat
     1141 agtggatagg gtaacagctt tgtttgctgc aacaaaaaac gtatcgcaca tacgaaatgc
     1201 ttcttcccca gatatacgaa tgagggcaat ggctgcttgg tgttgaccag aggaaatagc
     1261 acaaatagta ggattcataa gcggtatacc tcaactaata cacttttaag tagcacttgt
     1321 aaattatgag ttaacagtag gcaattcaac tagtacttct tgcacttgag ataaacaatg
     1381 tttagtttga caactagaaa cagatagcaa aagaattatg gcaattgcta ttgatgtgat
     1441 tatttttttc atattctttg tataaagagt tgggattagt tagaatagca acttacacca
     1501 tgctacattg gtaattattc atttgtttaa cgcaaatgta tgaaaaaaat tgtacatttg
     1561 cataatagaa acattattta ttcattaaaa acataaaaaa catgggactc ttaaaggaat
     1621 ttaaagcttt tgccatgaaa ggcaatgtca ttgacttagc tgtagctgta attattggtg
     1681 gtgcttttgg taaaattgta tcttcgttag tggcagatgt tattatgcct cctattgggt
     1741 tattggtagg tggggtaaat tttactgagt tgagtataat attgaaagaa gccacagaaa
     1801 ctaccgctgc tgttacttta aattatggta attttttgca agcaacgttt gattttatca
     1861 ttatagcttt tgctatattc atgtttatca aagggttaag taaattagaa aagaagaaag
     1921 aagagcctgt tgttcctgct gctccagttg aaccaacacc gtcaaaagag gaaattctat
     1981 tagcagaaat cagagatttg ttaaaagaaa aaaaataatc aatatcttca ttacaaaaaa
     2041 aaataaggaa cgtttaaacc aacgttcctt atttttttat gattgtactg tttctacagt
     2101 agctttatct gcttcgacct gacccaatcc acaatggcaa ggtgttttac acgagttgaa
     2161 taacatagct aaaaaagcac acacacatac tacttttatc agattttttt tcataatttg
     2221 gtatattagg ttttaattat tgtttgtaaa tcaatacggt agcatacgct gccattcctt
     2281 cttttctgcc tacaaaaccc atcttttcgg aggtagttgc tttaatggaa atatcctctt
     2341 ctgacacatg cattacctga gctagagttt ctttcatatt ggcaatatgt ggattgattt
     2401 ttggttcttc ggcacataca gtagcgtcaa tattactcaa ctcgtatcct tgctctctga
     2461 gtaagttcat cgtttttgcc agcaatattt tactatctat gtttttaaat tcgggcgatg
     2521 tgtcagaaaa atggtatcca atatctctca agtttgcagc tccaagtaag gcatcacata
     2581 tagcatgaat taatacatca gcatctgaat gccctagtaa acctaggcta tgttctatgc
     2641 taacaccacc taaccacaag gctctattgg gagcaaactg atgtacatca tacccaaaac
     2701 caacacgaat tttcatctgt cttatgtata aacaagtggt acttatcgag cagttagctg
     2761 tttgatgcca tctaaggtaa aggctaaact aaatcgtaaa gtttgatcca atgggttatt
     2821 ttgagccacc gaaacaacat aagctacatc caacgaaaac acattgagtt tgaaccctgc
     2881 accaatagtt gcatatttac gatttccttt gttggcactt tcgttaaaat atcctgcacg
     2941 agcaaaaaac tgattgttgt aggcatattc aagacccaat gaccacataa tttcttgcaa
     3001 ttcttcggag aaaccaccag gagcatctga gaatgaggta aatatacctg caatagggtc
     3061 cccattattg taatcactta aactagtctc atatttactc tcacgcaaag catagtcagc
     3121 atcactttca tcatctccct gagtaggagc aaccggacga gaaggcacca ataacttatt
     3181 aaggtcaaga ttaacagata atttgttgta tgggtcaagt ggataggtca acgaaccacc
     3241 t
//
LOCUS       DAOPUR010000143         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30627_length_3220_cov_3.278673, whole genome shotgun sequence.
ACCESSION   DAOPUR010000143 DAOPUR010000000
VERSION     DAOPUR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30627_length_3220_cov_3.278673"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1026
                     /locus_tag="PKN90_03375"
     CDS             79..1026
                     /locus_tag="PKN90_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNZ85353.1"
                     /translation="MDILLLLGGFVALVYGADKLVEAAASFAKKLQIPSIVIGLTIVS
                     FGTSAPEFVVNLFASLENNSDIVLGNVLGSNIANILLILGTSAIIYPLTVKKQTTWIE
                     IPLSVMAAIAVFFLASDQLIDHATQSAITHIDGMMLLLFFLVFIAYNISLTIKSKNDE
                     DIETKDYSILVSTLLIVLGLTLLGAGGKAIVYGATNLAETFGISQRIIGIIIVGIGTS
                     LPELATSVVAARKQKTDIAIGNIVGSNIFNTFFVLGTSATISSVQVSSAAMYDIYVNI
                     ACSVLLFGLLFIGKSHTISRKEGFIMLGLFITYIASITL"
     gene            complement(1033..>3220)
                     /locus_tag="PKN90_03380"
     CDS             complement(1033..>3220)
                     /locus_tag="PKN90_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNZ85354.1"
                     /translation="LLFKRLKQPMVLGYIVAGFLVGPYFDVLPTIIEKESVHQWSEIG
                     VIFLLFALGLEFSFKKLMHVGSSALITAGTEVVTMFSLGTLVGYLLHWTMIESFMLGG
                     MLSMSSTTIIIKAFDDLGIRKHKFTSIVFGTLVVEDIVAIVMMVLVSTAALSKEFAGI
                     DMLYSIVKLAFFLVLWFLVGIYVFPSFFQKARKWMNDENLLIISIGFCFGMVAMATYT
                     GFSAALGAFIMGSIFAETVEGEHIEKLSKGIKDLFGAIFFVSVGMMVDPSVLVEYWFP
                     VLLLTLSTIFGKAFFSFLGVLLSGQDLRTAIQSGMSLAQIGEFAFIIATLGYTLGIMS
                     PHIYPIIVAVSVISTFTTPYMIRLANPLYEWIYPRLSSNTKETLAKYVTYSNRVTTSS
                     DWKQLLRAFAVQVIGYTVICVGIILFMFHFVHPFLLEKLNVWMPNVLINLFHALLTIA
                     FIFPFLKGLMVNRNRKQDIFLKLWNENKVTRGGLMSLLLLRVFIAVVLVTFVLYSCFT
                     HSYLFVILVALAIVLFILFSRVSFLHYAKIEKRFISNLHTKEELDKQQKPLRTSFYSH
                     FAHQNIHLAVVETSVDSPYIGQKISETNIKEAFGVSIIKIIRGKNFIYLPTGEERIYP
                     YDQLLVLGNDEQIERLNTVMEQKEDIDIPKANIDVTLTSFIVQEGSSLIGKSIIQSGI
                     RNVGCMIIGIERHNLTIMNPDSSEIIQLADIIWIVGDKQNIQKTMC"
BASE COUNT         1131 a          604 c          468 g         1017 t
ORIGIN      
        1 aaaaaacagt catgtaattt ttttgttccc agtataaacc aatatctttg tatttcatta
       61 aatgtgaaga tagctatgat ggatatttta ttgttacttg gtggttttgt ggcattggtc
      121 tatggtgcag ataagcttgt tgaagcagca gctagttttg ccaaaaaatt acaaatacca
      181 agtatcgtta ttggtcttac aattgtttct tttggcacct ctgctcccga atttgtcgtc
      241 aatctgtttg cctcgcttga aaataacagc gatatcgttt taggaaatgt cttagggagt
      301 aatattgcta atattctact catactcgga acatcagcta ttatataccc tttaacagta
      361 aaaaaacaaa ctacttggat agaaatccca ctaagtgtta tggctgctat tgctgttttc
      421 tttttagctt cagatcaatt gattgatcac gctactcaat cagcaataac tcatattgat
      481 ggaatgatgt tattattgtt cttcttagtt tttatagcgt ataatatctc tcttaccatt
      541 aaaagtaaaa atgacgaaga tattgaaaca aaagattact ctattttagt ttcaactcta
      601 ctcattgtac ttggattaac attgttaggg gcaggaggaa aagctatagt gtatggagct
      661 actaatttgg cagaaacctt tggtatcagt caacgcatca ttggtattat tattgttgga
      721 ataggaacat cattacccga attagcaaca agtgtggtag ctgctcgtaa acagaaaaca
      781 gatatagcaa ttggcaatat tgtcggttct aatattttca atactttctt tgtattagga
      841 acttcagcta ccattagttc ggtacaagta tcttcggctg caatgtacga tatctatgtt
      901 aatatagcat gtagtgtctt gttatttggg cttttattta ttggaaagtc acatactata
      961 tctcgtaaag aagggtttat aatgcttggc ttgtttatta catatatcgc atccattaca
     1021 ttatagaaaa cctcaacaca tcgtcttttg tatgttttgt ttatccccta caatccaaat
     1081 aatatcagct agttgaataa tctcagacga atcgggattc attatggtaa ggttgtgtct
     1141 ctctattcca ataatcatac accccacgtt tctgatacca gattgaataa tacttttacc
     1201 gattaaagaa gagccctctt gtactataaa tgacgttaac gtaacatcaa tatttgcttt
     1261 tgggatatct atgtcttctt tttgttccat cactgtattc aaccgttcta tttgttcatc
     1321 attgcccaat accaataact gatcatatgg gtaaatacgc tcctctcctg tcggcaaata
     1381 aataaaattt tttcctctta taattttaat aatggataca ccaaaagcct ccttaatatt
     1441 agtttcacta atcttttgtc caatatatgg cgaatcaaca gatgtttcaa caaccgctaa
     1501 atgtatattt tgatgtgcaa aatgtgaata aaaacttgtt cgtaaaggtt tttgctgctt
     1561 gtctaattct tctttcgtgt gtaaatttga aataaatctc ttttctattt tcgcataatg
     1621 taaaaacgaa actctagaaa acaatataaa caagacaata gccaatgcta ctaaaataac
     1681 gaacaaataa ctatgtgtaa aacaagaata taatacaaaa gttactaata caacagctat
     1741 aaaaacacgc aacaataata atgacatcaa tcctcctcgt gtaactttat tttcattcca
     1801 tagttttaaa aatatatctt gttttctatt tctgtttacc attaatcctt ttaaaaaagg
     1861 aaatataaat gcaatagtca acaaagcatg aaaaagatta atcaatacat taggcatcca
     1921 gacatttaat ttttctagta gaaaaggatg aacaaaatga aacataaata aaatgatacc
     1981 aacacaaatc acggtatagc caattacttg cacagcaaac gctctcaata attgtttcca
     2041 atcgctacta gtcgtcaccc tatttgaata agttacatat ttggctaatg tttctttggt
     2101 attgcttgat aaacgcggat atatccactc atataaagga ttagctaatc gtatcatgta
     2161 aggagttgta aaggtagaaa taaccgatac tgctactata ataggatata tatgcgggct
     2221 cattatgcct aaagtataac ccaatgttgc tataataaac gcaaactctc cgatttgagc
     2281 taaactcata cctgactgaa tggctgtacg taaatcttga ccagaaagta acacccccaa
     2341 aaaggagaaa aaggctttgc caaagatcgt cgataacgta agcaataaca caggaaacca
     2401 atattcaact aatacagatg ggtcaaccat cataccaaca gatacaaaaa atatagcacc
     2461 aaataaatct ttaatgcctt tagacaattt ttcaatatgc tctccttcca ctgtttcggc
     2521 aaaaattgac cccattataa atgctcccaa tgcggctgag aaacctgtat aagtagccat
     2581 tgctaccatt ccaaaacaaa acccaatgga aataatcaac aaattttcat cgttcatcca
     2641 ctttcgagct ttttgaaaaa aagagggaaa aacataaatc ccaaccaaaa accacaatac
     2701 caagaaaaag gctaatttaa caatgctgta taacatgtca atacctgcaa attctttact
     2761 taaagcagct gttgatacta aaaccatcat aacaatggca actatatctt caacgactaa
     2821 ggtaccaaaa acaatagagg taaatttatg cttgcgaatt cctaaatcgt caaacgcctt
     2881 gataatgatg gtagttgatg acattgaaag cataccacca agcataaaac tttcaatcat
     2941 tgtccaatga agtaaatacc ctaccaatgt gcccaacgaa aacatcgtta caacttcagt
     3001 tcctgctgta ataagagctg aactgccaac atgcattagt tttttaaaac taaactcaag
     3061 ccccaatgca aacaaaagaa aaatcacacc aatttcagac cactgatgca cactctcctt
     3121 ttctataatt gtgggtaaaa catcaaaata aggtcccact aaaaatccag ccacaatata
     3181 tcccaaaacc ataggttgct taagtcgctt aaaaagcaac
//
LOCUS       DAOPUR010000144         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30676_length_3216_cov_3.528314, whole genome shotgun sequence.
ACCESSION   DAOPUR010000144 DAOPUR010000000
VERSION     DAOPUR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30676_length_3216_cov_3.528314"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKN90_03385"
     CDS             <1..1003
                     /locus_tag="PKN90_03385"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HNZ85355.1"
                     /translation="TITPDEARVVEFGLKKMWKSPNGTLRNIIGGTVFREPIICSNVP
                     RLVPNWTQPIVIGRHAFGDQYKATDVVIKGKGKLTMSFTNENGETTQWNVYDFEGDGV
                     AMCMYNTDDSIYGFARSSFQMALNKKWPLYLSTKNTILKAYDGRFKDIFQDVYEKEFK
                     ADFEANGLVYEHRLIDDMVAAALKWNGGFVWACKNYDGDVQSDTVAQGFGSLGLMSSV
                     LVTPDGKTVEAEAAHGTVTRHYRQHQQGKETSTNPIASIFAWTRGLAHRGKLDNNQAL
                     VNFCATLEKVCVETVESGKMTKDLAILVSGDNVSRKDYLSTQEFLAAIKENLDKALA"
     gene            1117..>3216
                     /gene="polA"
                     /locus_tag="PKN90_03390"
     CDS             1117..>3216
                     /gene="polA"
                     /locus_tag="PKN90_03390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNZ85356.1"
                     /translation="MKKLFLLDAYALVYRAYYAFIKNPRINSKGLNTSAIYGFVNTLE
                     DILKREKPSHIAIAFDPSGKTFRHEAYEAYKAQRESTPEDIRTSLPIIKNIIKAYGIP
                     IIEVPGFEADDVIGTLAKKAEKAGFITYMVTPDKDYGQLVSERIFMYRPKHTGGYETL
                     GIEEIKEKYQLASHEQVIDLLGLMGDSSDNIPGCPGVGEKTAVKLLQEFGSIDNLLAH
                     TEQLKGALKTKVEENKDLIAFSKFLATIRTDVPVELHESELILSEPDENQLRTLYEEL
                     EFRTFIAKMQASQASKPETSVSKKAPQQGNLFDMFEENDAEEERTAMGSTSTMDTIDT
                     IKPTYILLNNAKDIDAFVADLLSQKTVCFDTETTGIDINESKLVGMSFCWKKNTAYYI
                     PFTSFDETALFLEKIQPLFNNEHTLLIGQNLKFDLLMLQNYGVRVRAKLFDTMVAHYV
                     LQPELRHGMDYLAEIYLKYRTIHYEDVVGVKGKNQQNIQDIDPAIVCNYAAEDADITF
                     QLKEILEKELHANRLEKLFFDIEMPLVKTLCTIERAGVRIDTQALSQSSLVLTEQMQE
                     IEHNVQQHVGFEFNLSSAKQVGEVLFDRLKITDKAKKTKTGQYATSEDILEELRGKHP
                     IVPLILEYRGLKKLLSTYIDALPLLVNKHSGKIHTSYNQTITATGRLSSSNPNLQNIP
                     IRDAQGKEIRKAFIENNL"
BASE COUNT         1125 a          601 c          622 g          868 t
ORIGIN      
        1 taccatcacg cccgacgaag cacgcgttgt agaatttggt ctaaaaaaaa tgtggaaatc
       61 gcctaatggc acgctacgta atattattgg tggaaccgta tttcgcgaac caattatctg
      121 ctccaacgtg cctcgtttag tacccaactg gactcaacca attgttatcg gacgtcatgc
      181 ttttggcgac cagtacaaag ctaccgatgt agtaattaaa ggtaaaggta aattaaccat
      241 gtcgtttact aacgaaaatg gggaaacaac acaatggaat gtatatgatt ttgaaggaga
      301 tggcgtagct atgtgtatgt acaatacaga cgattctatt tatggatttg cacgttcttc
      361 ttttcaaatg gcattaaaca aaaaatggcc actgtatctg agcactaaaa acactatttt
      421 gaaagcttac gatggacgtt ttaaagacat ttttcaagat gtatatgaaa aagaatttaa
      481 agcagatttt gaagcaaatg gattggtata tgaacaccga ttaatcgatg atatggtagc
      541 agctgcacta aaatggaacg gcggatttgt gtgggcttgt aaaaattacg atggcgacgt
      601 gcaatcagac actgtggcac agggttttgg ttcgctaggt ttgatgtcgt cggtattggt
      661 aactcccgac gggaaaaccg tagaagcaga ggcagctcat ggcaccgtaa cgcgccatta
      721 tcgtcagcac caacaaggca aagaaacatc aacaaatcct attgcatcaa tttttgcttg
      781 gacgcgtgga ttagctcata gaggaaaact agacaacaat caagccttgg taaatttctg
      841 tgctacacta gaaaaagtat gtgttgaaac agtagaatca ggcaaaatga cgaaggattt
      901 agctatctta gtttctggag ataatgtttc tcgcaaagat tatttaagca cacaagaatt
      961 tttggctgca attaaagaaa acctagacaa agctttagca taagctttac taaattttta
     1021 tcatagatat tatgggaggg gctgtttgtt tctcccattt ttgtttgtat ctttgtaaga
     1081 tatgaactca taggatattc acctgataca attacaatga agaaattatt tcttttagac
     1141 gcttatgccc ttgtttacag agcgtattat gcgtttatta aaaaccctcg catcaactca
     1201 aaaggactca acacctcagc aatttacggt tttgttaaca ccttagaaga cattctgaaa
     1261 agagaaaaac caagccatat agcaatagct ttcgacccat ctggcaagac ttttagacac
     1321 gaggcttacg aggcatacaa ggctcaacga gaatcaactc ccgaagatat tcgcacgtcg
     1381 ttgcccatta taaaaaatat aattaaagcg tatggtattc ctattattga ggtgcctggt
     1441 tttgaagcgg acgacgtaat tggtactctc gcaaaaaaag cagaaaaagc gggttttatt
     1501 acttatatgg taactcccga caaagattat ggacaacttg tgtctgaacg tatttttatg
     1561 taccgaccca aacatacggg agggtatgaa actctaggaa tagaagaaat aaaagaaaaa
     1621 taccaattag cttctcacga acaagtaata gatttgctcg gattgatggg cgatagtagc
     1681 gataatatac cgggctgccc tggcgtgggc gaaaaaaccg ccgtcaaact gttacaagaa
     1741 tttggtagta ttgacaatct gttggctcat actgaacagc tgaaaggtgc cttaaaaacg
     1801 aaagtggaag aaaacaagga cttaatcgct ttctcaaaat ttttagccac aatacgtacc
     1861 gacgtacctg ttgaattaca tgaatcggaa cttatactga gcgaaccaga cgaaaatcaa
     1921 ttgcgtacac tatacgaaga acttgagttt cgcaccttta ttgcaaaaat gcaggcatca
     1981 caagccagta agccagaaac aagtgtttca aaaaaagctc ctcagcaagg caatttgttt
     2041 gacatgtttg aggaaaatga tgctgaagaa gaacgtacag caatgggatc aacttcaaca
     2101 atggatacca tcgatacaat taaacctacc tatattttac tgaataatgc gaaagatata
     2161 gatgcctttg tagcggattt attgtctcaa aaaacggttt gttttgatac agaaacaaca
     2221 ggcatagata tcaatgagag caaactagta ggcatgtcgt tttgttggaa aaaaaatact
     2281 gcttactaca taccttttac ttctttcgat gaaaccgcac tttttttaga aaaaattcag
     2341 ccactattta acaacgaaca taccctttta ataggacaaa acctaaaatt tgacctatta
     2401 atgttgcaaa attatggcgt tcgagtacgt gctaaactat tcgacacgat ggtagctcat
     2461 tatgtactcc aacccgaatt gagacatggg atggattact tagccgaaat ttacctcaaa
     2521 taccgcacaa ttcactacga agatgtagta ggagttaaag gaaaaaacca acaaaatata
     2581 caagatattg accctgctat agtatgtaat tatgcagcag aagatgccga cataactttt
     2641 cagctaaaag aaatactcga aaaagagctt catgccaatc gcttagaaaa acttttcttc
     2701 gatatcgaaa tgcctttagt aaaaacccta tgtactatcg aacgagcagg tgtacgtatt
     2761 gatacccaag cattgtctca atcttcgttg gtacttaccg aacaaatgca agaaattgaa
     2821 cacaacgtac aacaacacgt gggatttgaa ttcaatctta gttcggctaa acaagttgga
     2881 gaagtattgt tcgaccgatt gaaaattaca gataaagcga agaaaacaaa gacagggcaa
     2941 tatgccactt ctgaagatat tttagaagaa ttacgaggca aacatcctat tgtccctctc
     3001 atactcgaat accgaggatt aaaaaaactc cttagcacgt atattgatgc tttgcctcta
     3061 ttagtaaaca aacattcagg aaaaatacac acttcttata atcaaacaat tacagctacg
     3121 gggcgactaa gttcgagcaa tccgaacctg caaaatatac ccatacgaga tgctcaaggc
     3181 aaagaaataa gaaaagcatt tatcgaaaac aacttg
//
LOCUS       DAOPUR010000145         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30677_length_3216_cov_3.271433, whole genome shotgun sequence.
ACCESSION   DAOPUR010000145 DAOPUR010000000
VERSION     DAOPUR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30677_length_3216_cov_3.271433"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKN90_03395"
     CDS             complement(<1..586)
                     /locus_tag="PKN90_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNZ85357.1"
                     /translation="MKVVKFAILVPIVFVLACSSKEPKLADVVDTGVVGKMIKVEGGK
                     FMMGCSDCAVDEAPVREVTLSTFYIGEFEVLQEEWIAIMEEENPSYHQGDSLPVEQVS
                     WNQVSTFITKLNEKTGKTFRLPTEAEWEFAARGGIKSMGYTYSGSNDINEVAWYLDNN
                     EEQETQKVGQKLPNELGLFDMSGNVAEWVQDRYWV"
     gene            complement(605..>3216)
                     /locus_tag="PKN90_03400"
     CDS             complement(605..>3216)
                     /locus_tag="PKN90_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ85358.1"
                     /translation="FDATQGNDAMLGSATSYAHAGVILTSGPGWKKAPVWLDNASKYQ
                     LVSIGTDLWELTIPNLSSYYNLAEGEVIAQLAFVFRNADGTKQGKTAAGGDIFIDLYD
                     PSVLSMSVTNPSTDAVINKDASLVVQANVSMSSKIELFINSQLVKTEETATSISYSFD
                     TSVAGEYTVKIKATNGEDSVKISREICVKSPVQYAPVPTGMLEGINYDAQNNSVTLVL
                     RAPLKENVFVIGDFNEWKIKNQYQMFRNEENGILWWLTIDNLNPDTEYAFQYLIDGNL
                     KVSDAYTEKVIDPWNDKWIPTSVHPRLTYPEKADGLVSVFKINRPQYQWEVPDFQVPA
                     KEDLIIYELLIRDFTTEGTIKAAMGKLDYLQNLGINAIELMPIQEFDGNDSWGYNPNH
                     YFAYDKAYGTQEDYKRFIDECHKRGIAVFIDKVFNHATGVNPFAKMYWDGNATAANNP
                     WFNVVAKHPFNVFQDFNHEYEGTRQFFKRVLAYWIKEYNVDGYRMDLSKGFTQTNSGS
                     DAGKWGKKDISRINIIKDYHQAVKEANPNAVFILEHFADYDEEVILSTEGMLPWRNMT
                     SNYGQATMGYSSNSNFVDTNGKGGMFTNGWVGFAESHDEERLGYKMIAWGAGDLKTNE
                     SARLKRVPLVMAFVSFIPGPKMLWQFQEMGYDYSIEYGGRTSRKPIPWTAFNSNQAKQ
                     EAYEASAKIISLRTNYPQVFKEENVTVQATASYWSNGRRIVAQHPDMNVVAIGNFDAT
                     ATITATPGFTKTGTWYELLSGTVLNVKNVNRTISLAPGELLVYTDRIPVSVPETLQDE
                     TMTYVYPTIAKDYIYVSSLQDILQVNIYSLQGVLVKSEANSEIAISDLYSGYYIVQVV
                     QADGVSIHKINKQ"
BASE COUNT          949 a          704 c          473 g         1090 t
ORIGIN      
        1 acacccaata cctatcttgt acccattcgg ctacgtttcc actcatatca aataatccaa
       61 gctcgttggg aagtttttga ccaacttttt gggtttcttg ttcttcgtta ttatccaaat
      121 accaagccac ttcgtttata tcattgcttc cgctataggt atatcccatt gatttaattc
      181 ctccacgtgc agcaaactcc cattcagcct cggttggcaa acgaaatgtt ttacctgttt
      241 tttcgtttaa ctttgtaata aatgtggata cttgattcca cgatacttgt tccacaggca
      301 ggctatctcc ttgatgatac gatggatttt cttcttccat tatagctatc cattcttctt
      361 gtaacacctc aaactctcca atataaaagg ttgaaagggt aacttcacgt acaggagctt
      421 catcaactgc acaatcacta caacccatca taaattttcc gccttcaact ttaatcattt
      481 ttccgaccac acctgtatct actacatctg ccaattttgg ctctttggac gaacacgcca
      541 gaacaaatac aataggaaca agaatagcaa atttaactac tttcatacga tttttatttg
      601 agtattattg tttattaatt ttatgaatag aaacgccatc tgcttgcact acttgaacaa
      661 tgtaatatcc actatataaa tcacttattg caatttctga gtttgcttct gatttaacta
      721 aaacaccttg gagcgaataa atatttacct gcaaaatatc ttgtaacgaa gacacatata
      781 tataatcctt ggctatagtt ggatacacat aagtcatcgt ttcgtcttgt aacgtttcag
      841 gcacacttac aggtattcta tctgtataca caagtaattc tcctggagct aacgatattg
      901 ttctattcac attcttaaca ttcaaaaccg ttcctgataa taattcatac catgttccag
      961 ttttagtaaa acctggcgtt gcagttatag ttgcagtagc atcaaagttt cctatagcta
     1021 ctacattcat atctgggtgt tgtgctacta ttcttcttcc atttgaccaa taggaagcag
     1081 tagcttgtac agtaacattc tcctctttaa aaacttgagg gtaattggtt cgtaaactta
     1141 tgattttagc tgatgcctca taagcctctt gtttggcttg attagagtta aacgctgtcc
     1201 aaggtattgg tttacgtgat gttcttccgc cgtattctat cgaataatca tatcccattt
     1261 cttgaaattg ccataacatt tttggaccag gaatgaatga cacaaatgcc attaccaatg
     1321 gaactctttt taatctggct gactcatttg tttttaaatc accagcaccc caagctatca
     1381 tcttatagcc cagacgttct tcatcatggc tttctgcaaa accaacccat ccgttggtaa
     1441 acattcctcc tttaccattc gtatcaacaa aattactgtt acttgaatag cccatggtag
     1501 cttgaccata attactagtc atattcctcc aaggcaacat gccttctgta gaaagtataa
     1561 cttcttcatc ataatcagca aaatgttcaa gtataaaaac agcgtttgga tttgcctctt
     1621 ttacggcttg atggtaatct ttaataatat tgatacgcga tatatctttc tttccccatt
     1681 tacctgcatc actacccgaa tttgtttgag taaatccttt cgacaaatcc attctatatc
     1741 catccacatt gtactctttt atccaatatg caagtacccg tttaaaaaac tgtctagttc
     1801 cctcgtactc gtggttaaaa tcttggaaaa cattaaatgg atgtttcgca accacattaa
     1861 accaagggtt gtttgcagca gtagcatttc catcccaata catttttgca aaaggattaa
     1921 cccctgtagc atgattgaac accttatcga taaatacagc tataccacgc ttgtgacact
     1981 catcaataaa ccgtttataa tcttcttgtg tgccataggc tttatcataa gcaaaataat
     2041 gatttggatt gtagccccaa ctatcattgc catcaaactc ttgaattggc atcaattcaa
     2101 tcgcatttat acctaaattt tgcaaataat ctaacttgcc catcgccgct ttaatggttc
     2161 cttctgttgt aaaatcgcga ataagcaatt cataaataat caaatcttct ttagctggaa
     2221 cttgaaaatc tggaacttcc cattggtatt gtggtctatt tattttaaag acagaaacca
     2281 acccatcagc cttctctggg tacgttaatc gtggatgaac tgatgttggt atccacttat
     2341 cattccatgg gtcaattact ttttcagtat acgcatcact cacttttaga ttaccatcta
     2401 ttaaatactg aaatgcatat tcggtatctg gattaaggtt atcaatggtt aaccaccaca
     2461 aaatgccatt ttcttcattg cgaaacattt ggtattggtt ctttattttc cattcgttaa
     2521 aatcacctat aacaaacaca ttttctttta atggggctct aagtactaaa gtaacactat
     2581 tattttgagc atcataatta atcccttcta gcatacctgt tggtacgggt gcatattgta
     2641 caggagattt cacacaaatc tctctactta ttttaacact gtcctctccg tttgttgctt
     2701 taattttcac agtatattca ccagcaacac ttgtatcaaa gctataagaa atagaagtag
     2761 cggtttcctc tgtttttact aactgactgt ttataaatag ctctatttta gaagacattg
     2821 atacatttgc ttgcacaacc aaagacgcat ccttatttat tacagcatct gtcgaagggt
     2881 tcgtaacgct catactcaac acagaaggat cgtataagtc aataaagata tcgccaccag
     2941 cagctgtttt gccttgcttg gttccatcgg catttctaaa tacaaaagcc aattgtgcaa
     3001 ttacttcgcc ttcagccaag ttgtaataag atgacaaatt tggaattgtt aactcccaca
     3061 aatctgtccc aatggaaaca agttgatatt tagacgcatt gtcaagccaa actggagctt
     3121 ttttccatcc tggacctgag gttagtataa cccctgcatg cgcataagat gtagctgacc
     3181 caagcatggc atcattacct tgagtagcat caaaaa
//
LOCUS       DAOPUR010000146         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_30786_length_3209_cov_3.170894, whole genome shotgun sequence.
ACCESSION   DAOPUR010000146 DAOPUR010000000
VERSION     DAOPUR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30786_length_3209_cov_3.170894"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKN90_03405"
     CDS             <1..439
                     /locus_tag="PKN90_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85359.1"
                     /translation="KASVLYGQNLSELTMPGGYAVQTHDVATGHRTYTPSNAVSSWIN
                     FTYGKKWIAGIFGGYHKNLGFSDNILDSTANGALFGRWQNIDHIYRASTSLTYKIERW
                     VFGAELDYNVAAYGEVDYGKKGKIKNATETSNIRGVLSATFIF"
     gene            592..804
                     /locus_tag="PKN90_03410"
     CDS             592..804
                     /locus_tag="PKN90_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNZ85360.1"
                     /translation="MEKITNWIKKNRLYFVGSILGGIGGYIYWYFIGCDSGGCPITSS
                     PYNSVIWGAIMGSLLLSMFKKEKQDE"
     gene            797..1036
                     /locus_tag="PKN90_03415"
     CDS             797..1036
                     /locus_tag="PKN90_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85361.1"
                     /translation="MNKYLKNWTILRIIRLLLGASLSIAAYFSGHYLFVGLGVWFMIQ
                     AFFNVSCCGTAGCDSETPSNSSDTVFKNEIKEYKK"
     gene            1033..1527
                     /gene="trxA"
                     /locus_tag="PKN90_03420"
     CDS             1033..1527
                     /gene="trxA"
                     /locus_tag="PKN90_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNZ85362.1"
                     /translation="MKQLTLLAIAIGLLVTSCSEASNKEQKQPTPTEKKIQTTNNTLI
                     MKPIYLTKAEFLKKVVNYETNPNEWKYLGDKPAIIDFYASWCGPCKMIAPILDELAAE
                     YAGEIYIYKIDTEQEQELAAAFGIQSIPTLLLVPMNENPQIIRGALPKASFKEAIDKV
                     LLKK"
     gene            1533..1898
                     /locus_tag="PKN90_03425"
     CDS             1533..1898
                     /locus_tag="PKN90_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ85363.1"
                     /translation="MESLCYIRNIYRLISKFEEQLQQKHELSLNEGMVLCELKNASYS
                     SGEIANLVSLTHSNTSKVIAALEKKQLVTRIIGDTDKRKMFFSITPQGTQKVNELLCE
                     ESEIQRIAKNIESDILQEK"
     gene            2063..2431
                     /locus_tag="PKN90_03430"
     CDS             2063..2431
                     /locus_tag="PKN90_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNZ85364.1"
                     /translation="MKAIYTILLLLAANIFMTFAWYGHLKLKDFKWFSALPLIAVILF
                     SWGIALFEYFFQVPANKIGFRENGGPFTLLQLKVIQEVITLVVFVAFSSLFFKTETFR
                     WNHAIGFMFLVLAVYFIFKK"
     gene            2543..2881
                     /locus_tag="PKN90_03435"
     CDS             2543..2881
                     /locus_tag="PKN90_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNZ85365.1"
                     /translation="MNSENIKSQMRKGYLEFCILLILEKKPAYASDILNELKAAKLIV
                     VEGTLYPLLTRLKNSGYLDYNWIESLQGPPRKYYELTENGKQFLSEIEEAWQEIKNVV
                     EHIKQQPNNE"
     gene            2935..>3209
                     /locus_tag="PKN90_03440"
     CDS             2935..>3209
                     /locus_tag="PKN90_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HNZ85366.1"
                     /translation="MKKTVTINLNGIVFHIDEDAYMLLQHYLEQLSKQFSAEDEKEIL
                     NDIEARIAELFTEKLQNGKNVVEKPDVERVMEVLGNPSQFADNDNDDT"
BASE COUNT         1129 a          568 c          548 g          964 t
ORIGIN      
        1 caaagccagc gtattatatg gacaaaattt aagcgaacta actatgcctg gtggttatgc
       61 tgtacaaaca cacgatgtag caacaggaca ccgcacctat acgccatcga atgctgtatc
      121 ttcatggata aactttacgt atggtaaaaa atggatagcc ggtatttttg gaggttacca
      181 caaaaattta gggttctctg ataacattct tgattcaact gccaatgggg ctttgtttgg
      241 tcgctggcaa aacattgacc atatttaccg tgcatctact tcgcttacat ataaaataga
      301 acgttgggtt tttggggctg agctcgatta caatgtggct gcctacggag aggttgatta
      361 tggcaaaaaa ggaaaaataa aaaatgctac cgaaacatct aacataagag gggttttatc
      421 agctactttt atattttaga aagtttatta cacaccatca tataatcaag gttacatact
      481 ctttttttaa ccttgattat tttttttgtt aaaatatatt ttccattgga aatttgtttc
      541 attgtaaaat aatacctatt tttgcagtgt aaaaataaca acaagctaca catggaaaaa
      601 ataacaaact ggataaaaaa aaataggctg tacttcgttg ggagcatact aggaggtatt
      661 ggtggatata tctactggta tttcatcgga tgcgattcag gcggatgccc tataacctca
      721 tcgccctaca acagtgtaat atggggtgct attatgggtt cgctcctatt aagcatgttt
      781 aaaaaagaga agcaagatga ataagtacct caaaaattgg acgattctac gtataatcag
      841 attgctttta ggtgcttctt tgagcattgc cgcgtacttt tctggacatt atctttttgt
      901 gggattagga gtatggttta tgattcaagc attttttaat gtatcttgtt gtggtactgc
      961 tggttgcgat tccgaaacgc cttctaatag ttctgataca gtttttaaaa atgaaataaa
     1021 agaatataaa aaatgaaaca actaacctta cttgccatag ccataggctt attggttaca
     1081 tcttgttccg aagcctcgaa caaggaacaa aaacaaccaa caccaactga aaaaaaaata
     1141 caaacaacta ataatacatt aattatgaaa ccaatctatt taacaaaagc agagttttta
     1201 aaaaaagtgg ttaattacga aaccaatccc aacgaatgga aatatttggg agacaaaccc
     1261 gcaataatcg acttctatgc atcatggtgt ggtccatgta aaatgatagc tcccatcctt
     1321 gatgaattgg cagcagaata tgcgggagaa atttacattt ataaaataga cacagaacaa
     1381 gaacaagaat tggcagctgc atttggcatt cagtcaatac caaccttgtt gcttgtacct
     1441 atgaacgaaa acccacaaat tattcgaggt gcattaccca aagcgtcatt taaagaagcc
     1501 attgacaaag tgttgcttaa aaaataatca aaatggaaag tctgtgttac atacgaaata
     1561 tttaccgatt gattagcaaa tttgaagaac agttgcaaca aaaacacgag ctcagcctca
     1621 acgagggcat ggtattgtgc gaattgaaaa atgccagcta ctcttctgga gaaatagcca
     1681 atctagtatc attaacacat tctaatacat ccaaggtaat cgcagctctt gaaaaaaaac
     1741 aactagtaac acgcataata ggtgatactg acaaaagaaa aatgtttttt tcaatcaccc
     1801 cacaagggac acaaaaagta aacgaacttc tttgtgagga aagtgaaatt caacgtattg
     1861 cgaaaaatat tgaatcagat atcttacaag aaaaataaga agaaatttgt ttcacactct
     1921 atcctttttt acaggcatat tcgtgtcatg taaaaaagga ttttttgtat cataaaatga
     1981 tggttgatta tacaaaaaat acaaaaactt tgtactacat ttgcacaatt tttagtgcat
     2041 cttcttttaa taaaagaact atatgaaagc catttatacc attcttttgt tgcttgctgc
     2101 caatattttt atgacttttg cttggtatgg tcatttaaag ttgaaagatt ttaaatggtt
     2161 ttcggcattg cctcttattg ctgttatctt atttagttgg ggaattgctc ttttcgagta
     2221 tttttttcaa gttcctgcca ataaaattgg ctttcgcgaa aatggaggac cctttaccct
     2281 gctccaactc aaagtgattc aagaagtaat taccttagtc gtttttgttg ctttctctag
     2341 cttattcttc aaaaccgaaa cctttcgctg gaaccacgcc ataggcttta tgttcttggt
     2401 attggctgtg tattttattt tcaaaaaata aattgctttc tatttttttg ttaatatttc
     2461 ttaatttata gtagtatgta ttgcatagta ctataaacac acttattttt gcactatcaa
     2521 cttacataca aacgctatgt caatgaattc ggaaaatatt aaatcgcaaa tgcgaaaagg
     2581 atacctagaa ttttgcattc tacttatcct cgaaaaaaaa cctgcctacg catcggacat
     2641 actcaacgaa cttaaagccg ctaaactaat agtagtagag ggcacattgt atcctttgct
     2701 aactcgactc aaaaacagtg ggtatcttga ctataactgg atagaatcgc tacaaggtcc
     2761 accccgtaaa tactacgaac tgacggaaaa tggcaaacaa tttttgtccg aaatagaaga
     2821 agcttggcaa gaaataaaaa atgtagttga acacatcaaa caacaaccca acaacgaata
     2881 gacagaacac ttcatcatca ataacaatcc ctcaaactac aaacatatac tcccatgaaa
     2941 aaaacagtaa caatcaacct caacggcatc gtttttcata tagacgaaga tgcgtacatg
     3001 ttgcttcaac attacttaga gcaattaagt aaacaattta gtgccgaaga tgaaaaagaa
     3061 attctcaacg atattgaggc tcgtattgca gaattattta ccgaaaaatt acaaaacggt
     3121 aaaaatgtag tcgaaaaacc tgacgtagaa agggtaatgg aagtgcttgg caatcccagc
     3181 caatttgctg acaacgacaa tgatgatac
//
LOCUS       DAOPUR010000147         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_31013_length_3192_cov_2.856870, whole genome shotgun sequence.
ACCESSION   DAOPUR010000147 DAOPUR010000000
VERSION     DAOPUR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31013_length_3192_cov_2.856870"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKN90_03445"
     CDS             complement(<1..453)
                     /locus_tag="PKN90_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNZ85367.1"
                     /translation="MIWNEHIECMSREQLRDLQTARLKETVERVYHNTPFYRKKMQEL
                     GILPSDIKDIDDIQKLPFTTKEDLRDNYPFGLMAVPMSEVVRIHASSGTTGKPTVVAY
                     TRKDVSQWTECVSRCFAAFGATKQDIFQISYGYGLFTGGLGAHYGTENI"
     gene            584..766
                     /locus_tag="PKN90_03450"
     CDS             584..766
                     /locus_tag="PKN90_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNZ85368.1"
                     /translation="MKTNKLTRSDERVIGGVCGGIAEYFDLDVSLIRVVWALVALIGG
                     TGLLAYLIAWLIIPQK"
     gene            787..2889
                     /gene="ovoA"
                     /locus_tag="PKN90_03455"
     CDS             787..2889
                     /gene="ovoA"
                     /locus_tag="PKN90_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HNZ85369.1"
                     /translation="MKELITKTIDLQSGNPEEKREEIRAYFLKTWALDELLYTQLRDD
                     SIFYHRGDPLRHILLFYLGHTAVFYINKLFLAKIIHQRINPEFESLFAIGVDEMSWDD
                     LNDKNYNWPSVGAVRAYRNQVKELLIHTIDTLPISLPIDWEHPFWIIMMGIEHERIHI
                     ETSSVLIRQLPLEEVVSGRFGNTCTLSGTAPRNEMIPVKGDTVVMGKPSNHHLYGWDN
                     EYGQHKELVADFSASKYLVSNAEYLGFIEENGYNTANYWTEEGWKWRTYTQAEMPLFW
                     RKKGNKFWLRLVAEEIEMPWNWPVEVNYLEAKAFCNWKTHKEGKTYRLPTEAEYVQLT
                     RVCAVPDELEWETAPGNINLEHFTSACPVDMFALGEFYDVIGNVWQWTETPITGYAGF
                     KVHPMYDDFSTPTFDGKHNLIKGGSFISTGNEATHHARYAFRRHFYQHAGVRLVQSET
                     PLVIQHAEYETDSEIANECESQWNTEDATNFDVQLAAYIKEQISHLDTKKVLDINTNT
                     GRLPFLLAQSVAKITAIDFSARFIRMPIQLQENGFMRYIVTDENELVHYREIVIDSHL
                     QEIAKEKIVFLQDNANNLKPIHSGFDVIIANNVLEELNNPIQFLQTIHQRLHKQGILI
                     VASSYNWKKNNIPAENWLGGYKKDGEPVTGLDAIKENLQNTFVLLDKPFDLQKIAYIS
                     TRESSVQRCEVSTWQKKS"
     gene            3010..3189
                     /locus_tag="PKN90_03460"
     CDS             3010..3189
                     /locus_tag="PKN90_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNZ85370.1"
                     /translation="MEEDIIICHCNEIYKSEIVKAIKEKGLTTVDEVGDETTAGTICG
                     QCQDDIQDILDEINK"
BASE COUNT         1064 a          567 c          632 g          929 t
ORIGIN      
        1 aatattttcg gtaccataat gagcacccaa acctcctgta aacaatccat agccatacga
       61 aatctgaaaa atatcttgtt tggtggcacc aaaagcggca aaacagcgag aaacacactc
      121 agtccattgc gacacatctt tgcgtgtgta cgctaccaca gtaggtttac ctgtagtacc
      181 cgaagaggca tgaatgcgta ctacctctga cataggtact gccattaatc caaaaggata
      241 attatcacgt aaatcttctt tggtggtaaa aggtaatttc tggatatcat caatatcctt
      301 aatatcagag ggtaagatac ccaattcttg catttttttg cgataaaaag gtgtgttgtg
      361 gtacactctt tcaactgttt cttttaggcg agctgtttgt aaatctctta actgctcgcg
      421 actcatacat tcaatgtgtt cgttccagat catatagtat tagattaaaa ggcaaatata
      481 catatttaat ttatcttaac acaaatattt gaaacaattt atttcttgat tggtttctaa
      541 aagatattga ttatttttgt gattaaataa ttaacgaaac gatatgaaaa caaataagct
      601 tacacgttct gatgagcgag ttattggtgg agtttgcggt ggaatagccg agtattttga
      661 tttggatgtt agtttaattc gcgttgtatg ggctttggta gctctaatag gtggcacagg
      721 gcttctggct taccttatcg cttggctaat aatcccccaa aaataaccaa tcaggagtag
      781 taatacatga aagagttaat cacaaaaacc attgacctgc aatcgggtaa tcctgaagaa
      841 aaacgcgaag aaatacgtgc gtatttttta aaaacctggg ctctcgacga actgttatat
      901 acacaattgc gtgatgatag tattttttat cacagaggcg atcctttgcg gcatattttg
      961 cttttttatt tggggcacac tgctgttttt tatatcaata agttgttttt agcaaaaatt
     1021 attcatcaac gaattaatcc agagtttgaa tcattatttg ccataggtgt tgatgaaatg
     1081 agttgggacg atttaaacga taaaaactat aattggccct ctgtaggtgc tgtaagggca
     1141 taccgcaacc aagtaaagga attactaatt cataccattg ataccctacc catctcgctt
     1201 cctattgatt gggaacatcc tttttggatt attatgatgg gcattgaaca cgaacgcatt
     1261 cacatcgaaa cttcatcggt acttattcgt caattacctt tggaagaggt tgttagtgga
     1321 cgatttggca acacctgcac cttatctggc acagcaccaa ggaatgaaat gatacctgta
     1381 aaaggcgata ctgtagtaat gggcaaacca agcaaccatc acctttacgg ttgggacaat
     1441 gaatacggac aacacaaaga actagtagct gattttagtg ctagcaaata cttggtgtca
     1501 aatgccgaat accttggttt tattgaagaa aatgggtaca atacagcgaa ctactggaca
     1561 gaagagggtt ggaaatggcg aacatataca caagcagaaa tgcccctgtt ttggagaaag
     1621 aaaggcaaca agttttggct acgtttggta gccgaagaaa ttgaaatgcc ttggaactgg
     1681 cccgtagaag ttaattacct cgaagccaaa gctttttgca actggaaaac acacaaagaa
     1741 gggaaaactt atcgcttgcc tactgaagcc gaatatgtgc aactaacacg cgtatgtgct
     1801 gtacccgacg aattggaatg ggaaacagct ccaggtaata tcaatttaga gcattttact
     1861 tcagcttgtc ctgttgatat gtttgcctta ggtgagtttt acgatgtaat tggcaatgtg
     1921 tggcaatgga ctgaaacgcc tattacgggc tatgcagggt ttaaggtaca ccctatgtac
     1981 gacgattttt ctactccaac tttcgatggg aaacataatt taatcaaagg tggttcgttt
     2041 atatctactg gcaacgaagc tactcaccat gctcgctatg cgtttagacg ccatttttac
     2101 cagcatgctg gcgtgcgttt ggtacaatct gaaacgccat tagtaattca acatgctgaa
     2161 tacgaaacag atagtgaaat tgcaaatgag tgcgaatcgc aatggaatac cgaagatgct
     2221 actaactttg atgtacaatt agccgcttat atcaaagaac aaattagcca tttagatact
     2281 aaaaaagtgc ttgatatcaa taccaatacg ggcagacttc cgtttttgtt ggcacaatct
     2341 gtagcaaaaa ttactgccat tgatttttcg gcacggttta tacgtatgcc catacagttg
     2401 caagaaaacg gctttatgcg gtacattgta acagacgaaa acgagttggt gcactaccgc
     2461 gaaattgtaa ttgacagcca tttgcaagaa attgccaaag aaaaaattgt gtttttgcaa
     2521 gacaatgcca acaaccttaa acctattcac agtggatttg atgtaataat tgccaacaat
     2581 gtattggaag aattgaataa ccctatccag tttttgcaaa ccattcatca acgattacat
     2641 aagcaaggca tattgatagt ggcttcaagc tataattgga aaaaaaataa catacctgca
     2701 gaaaattggt tgggcggcta caaaaaagat ggcgaacctg taacaggact ggatgccata
     2761 aaagaaaatc tgcaaaatac ctttgttctg cttgataaac cctttgattt gcaaaaaatt
     2821 gcgtatatct ctacaagaga atcaagtgtt caacgatgtg aagtaagtac atggcagaaa
     2881 aaaagctagt aagttttata aacaatatat aaagtaaatt aactaaaata attgcgattt
     2941 ttaatcactt tgctatacct ttgtttttag agtgaatgaa ataaattact aatttaacta
     3001 gtagccatta tggaagaaga tataatcatt tgccattgta atgagattta caaaagcgaa
     3061 atagtaaaag cgattaaaga aaaaggctta actaccgtag atgaagtggg cgacgaaacc
     3121 acagcaggca ctatttgcgg tcaatgccaa gacgatatcc aagatatttt ggatgaaata
     3181 aacaagtaaa aa
//
LOCUS       DAOPUR010000148         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_31182_length_3179_cov_3.589949, whole genome shotgun sequence.
ACCESSION   DAOPUR010000148 DAOPUR010000000
VERSION     DAOPUR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31182_length_3179_cov_3.589949"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..2217
                     /locus_tag="PKN90_03465"
     CDS             373..2217
                     /locus_tag="PKN90_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNZ85371.1"
                     /translation="MAEQIKKDNQDNIVIRFSGDSGDGMQLAGTLFANISAMSGNDIS
                     TFPDFPSEIRAPQGTLSGVSGYQVHIGPKVYTPGDKTDVLIAMNPAALKINVKNLKNS
                     SVVVIDTDSFTKKDLEKALFSTSNPFEELGIGNISVIEAPIGSMVKESLTNSGLEGKD
                     VLRCKNMFALGLSCWLFDRPLNLAESLIRTKFAKKPIVAEANIKVLHDGYNFGANTHA
                     SVPTYHIATSTKEKGFYTDVDGNTATAYGLIAAAEKAGMELFLGSYPITPATDILQEL
                     AKHKALGVKTMQMEDEIAGICSAIGASFAGSLAATNTSGPGLALKSEAIGLAVMAELP
                     IVIIDVQRGGPSTGLPTKSEQTDLMQALYGRNGESPVAVVAAKSPTDCFDAAFWASKI
                     AVEHMTPVILLTDGYIANGSGAFKIPELENYPSIQPHYIKKDQQNWKPYTRNEETKVR
                     YWATPGTEGFQHRIGGLEKDYTTSIISTDPANHQLMVETRQEKINAIANFIPIIDIQG
                     DANADVLIVGWGGTYGHLLSAVNEMNENGKKTAFAHFSFISPLPKNTEEILRKYKKII
                     VCEQNLGQFAGYLRMKIHGLPIYQYNEVTGQPFLVSELVTYFTKIMEE"
     gene            2222..>3179
                     /locus_tag="PKN90_03470"
     CDS             2222..>3179
                     /locus_tag="PKN90_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNZ85372.1"
                     /translation="MSELTYKPFQASDYKSAQAVRWCPGCGDHFLLNSLHKAMANIGI
                     PPHQIAVISGIGCSSRLPYYMNTYGFHTIHGRGAAIASGLKVANPKLSVWQITGDGDC
                     LAIGGNHFIHSIRRNMDLNIVLVNNQVYGLTKGQYSPTSNRGYVCKSAPYGTVEDPFR
                     PAELTFGARGTFFARTVDVDSPVAVEVLTAAAKHKGASVVEVLENCIIFNDGTHSAVS
                     KKEGRAKNAIVLQHGKPMIFGENNEKGLILDGMNLKVVNLGENGVTEKDILIHDETTI
                     DFTLHLKLATMPFPEFPIALGVIRSIKAPTYDEEITVQIKDVQ"
BASE COUNT         1109 a          561 c          599 g          910 t
ORIGIN      
        1 tttgtgtcat ttaagtaaag agaacaactc gcccgaatgt gcactatcta ccatcgaaaa
       61 aaaaattgcc catcaaagat ttaatttatg gactaattgt actctaatag ctttacctcg
      121 tactacccct actgaagttt ttattattta aaagacgatt atagttactt ttcgttacat
      181 ccaagctatt tttggcgtcc gtttttgaaa taattctttg aaaaatgaat tttataccgt
      241 atttttgtgc tcccaaaata ccatgacagt tcagataaac aaaatagtac gactacatca
      301 atcaatatca gaagtattcg tacgaatact taacaataag taatccacgt aaaaaagaat
      361 gaatactatc ctatggcaga acaaataaaa aaagacaatc aagacaatat tgtcatccga
      421 ttttcaggag actctggaga tggaatgcaa ttggcgggca cactttttgc caacatatcg
      481 gcaatgagtg gaaatgatat ttctacattt cctgatttcc cttccgaaat tagagcccct
      541 cagggtacat taagcggtgt atcaggctac caagtacata taggacccaa agtatatact
      601 ccaggagata aaactgacgt actaatagca atgaatcctg ctgcattaaa gataaatgta
      661 aaaaacctga aaaatagttc tgttgtagtt atcgacactg atagttttac taaaaaagat
      721 ttagaaaaag cattattttc aacgtctaac ccctttgaag aacttggaat aggaaatatc
      781 agcgtaattg aagctcctat aggatctatg gtaaaagaaa gccttactaa ttcaggatta
      841 gaaggtaaag atgtgttacg atgcaaaaat atgtttgcat taggtttatc gtgttggttg
      901 tttgatcgcc cattaaactt agcagagagc ttaattcgta caaaatttgc aaaaaaacca
      961 attgtagccg aagcaaacat aaaggtacta cacgatggat ataattttgg agctaacacg
     1021 cacgcttctg tgcctactta ccatattgct acaagtacaa aagaaaaggg attttacact
     1081 gacgtagatg gtaataccgc aactgcttat ggtttaattg cagctgccga aaaagcgggc
     1141 atggaattat tcttaggaag ctatcctatt acacctgcta ccgatatttt acaagaactt
     1201 gctaaacata aagcgttggg agtaaagacc atgcaaatgg aagatgaaat agcgggtatt
     1261 tgctcagcta ttggtgcaag ttttgctggt tcgttagctg ctacaaacac ttccggacca
     1321 ggtttagctc ttaaaagcga ggctattggc ttagctgtta tggcagaatt gccaattgta
     1381 attatagatg ttcaacgggg aggaccttca acaggcttgc caaccaaaag cgaacaaaca
     1441 gacttaatgc aagcgttata cggtcgcaat ggagaaagtc cagtggctgt ggtagcagca
     1501 aaatcgccaa cagactgttt cgatgctgct ttttgggcat ccaaaatagc tgtagaacac
     1561 atgaccccag taattttact cactgatggt tatatagcaa atggttccgg agcatttaaa
     1621 attcccgaat tagaaaacta tcctagtatt caacctcatt atatcaaaaa ggatcaacaa
     1681 aactggaaac catacacacg taacgaagag actaaagtgc gttattgggc aacacctgga
     1741 acagaaggtt ttcagcatcg cattggtgga ttagaaaaag attatacaac aagtattatt
     1801 tcaacagatc cagccaatca ccaacttatg gtggaaacac gtcaagaaaa aattaatgca
     1861 attgcaaatt ttatccctat tattgatata caaggagatg caaacgctga tgtattaata
     1921 gttggttggg gtggaacata cggacatcta ttatcagctg taaatgaaat gaatgagaac
     1981 ggtaaaaaaa cggcatttgc tcattttagt tttattagcc cattacctaa aaatacagaa
     2041 gaaatactgc ggaaatacaa aaaaataatt gtttgcgaac aaaatcttgg tcagtttgca
     2101 ggttatttac gcatgaaaat tcatgggtta cctatctacc aatacaacga ggtaactggg
     2161 caaccttttt tagtaagcga attagtaacg tatttcacaa aaattatgga ggaataaaaa
     2221 tatgtcagaa ttaacatata aaccatttca agcatccgat tataaaagtg cacaagcagt
     2281 acgctggtgt ccaggttgtg gcgaccattt tttattaaat tctctacaca aagcgatggc
     2341 taacattggg atacccccgc atcaaatcgc agttatttct ggtataggtt gttcttctcg
     2401 attaccttat tatatgaata cctatggttt tcataccatt cacggtcgtg gtgctgctat
     2461 tgcatcgggc ttaaaagttg ccaatccaaa attaagtgta tggcaaatta ctggtgatgg
     2521 cgattgtctt gccattggag gaaaccattt tattcattcc atacgtagaa atatggattt
     2581 aaatattgta ttagttaata atcaagtata tggcttaaca aaaggtcagt attcccctac
     2641 ttctaatcgc ggttacgtct gtaaatcagc tccctatgga acagtagaag atccttttcg
     2701 tcctgctgaa ttaacatttg gagctagagg tacttttttt gcacgtacag tagacgtaga
     2761 ttctcctgtg gcagtagaag tattaacggc agcagccaaa cacaaaggag cctcagtagt
     2821 agaagtatta gaaaattgca tcattttcaa tgatggaacg cacagtgctg taagtaaaaa
     2881 agaaggacgt gcaaaaaatg ccattgtatt acaacatggc aaacctatga tttttggcga
     2941 aaataacgaa aaaggattaa tcctagatgg tatgaaccta aaagtagtta atttaggtga
     3001 aaatggtgtt acagaaaaag atattctaat acatgatgaa acaaccatag attttaccct
     3061 acatctaaaa ctagcgacta tgccatttcc tgaatttccg attgctttag gagtaattag
     3121 aagtataaaa gcaccaacct atgacgaaga aataactgtg caaattaaag atgtacaag
//
LOCUS       DAOPUR010000149         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_31236_length_3175_cov_3.574679, whole genome shotgun sequence.
ACCESSION   DAOPUR010000149 DAOPUR010000000
VERSION     DAOPUR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31236_length_3175_cov_3.574679"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKN90_03475"
     CDS             complement(<1..294)
                     /locus_tag="PKN90_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HNZ85373.1"
                     /translation="MDFLMHNNLIGVLIGISTFCIIGLFHPIVIKCEYYFGTRCWWLF
                     LLIGVVGICMSIIVKHILFSSLLAVFAFSSFWSILEIFEQKKRVDKGWFPKNPK"
     gene            complement(298..1527)
                     /locus_tag="PKN90_03480"
     CDS             complement(298..1527)
                     /locus_tag="PKN90_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNZ85374.1"
                     /translation="MKYKICILFFYVSFTLFSQVVKTGIDVLQQRNFDVLSGKRVGLI
                     TNPTGVNKNLKSTVDILHEATNVKLIALYAPEHGVRGDIPAGEKVSNLIDTKTGVKVY
                     SLYGNTRKPTQEMLQGIDALVYDIQDIGCRSYTFISTLGLAMQAAAEKKIPVYVLDRP
                     NPLGGLKIEGNTTEDSFISFVSQFKIPYIYGLTVGELAYMLNEENMLGAKCELHIVPM
                     EGWKRSMRFNETELPWVLTSPHIPYMQSAVYYPASGILGELGIMSIGVGYTVPFQTFA
                     HEIFDAEKLSSSLNALRLNGVVFRPIHYKPYYGYGKDKNLKGVQVFFTNYTTARLSEV
                     QFYVLQVVKQLYPEFEVFTNENSSRWNMFDKVCGSDFIRIELQKNNYSFDKIKHYWRK
                     DEEDFKKKSTKYWIYKE"
     gene            complement(1517..2461)
                     /locus_tag="PKN90_03485"
     CDS             complement(1517..2461)
                     /locus_tag="PKN90_03485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ85375.1"
                     /translation="MLSVLIPIYNQNCSKLIYALQHQIDQLDVPVEIVAIDDSSIVYK
                     EENRKALNNTNHRYIELPNNIGRAAIRNKLATIAKYDYLLFMDCDTYPASDYYLHNYI
                     QCLQSNSYQNGVICGGYKYSLQPPVDTQQLFRWKYGRNREQNLAVERNKFPYKSFSSF
                     NFCIPSKVFASIQFDETIKGYGHEDTWFGIALKENKVPVIHIDNELFQVSIDKTDIYL
                     EKSRNAVKNLYTLYTTSDKMSMFEQIKLIRVFEKIKKMRLCAMGAFLYDVSKENLEKN
                     FHSHHPLLVLFDFYRLGLLCKESLLARNNSLLPNKDEV"
     gene            complement(2591..2917)
                     /locus_tag="PKN90_03490"
     CDS             complement(2591..2917)
                     /locus_tag="PKN90_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNZ85376.1"
                     /translation="MTEATKDSEFVDFAVTARKYKTLLTKKFKNRKVWEAPNPNNIKS
                     YIPGTIIELNVKPGQKVKQGELLLRLEAMKMQNRIDMPFDGIIKSVNVNVGEKIPKNF
                     LMIEIE"
     gene            complement(2981..>3175)
                     /locus_tag="PKN90_03495"
     CDS             complement(2981..>3175)
                     /locus_tag="PKN90_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNZ85377.1"
                     /translation="DAMRAAKVQEYVDKFANPFVAAAKGYIDAVIEPNETRNLLIHTL
                     SVSKDKDDYRPEKKHGIPPF"
BASE COUNT         1058 a          581 c          406 g         1130 t
ORIGIN      
        1 tttaggattc tttggaaacc accctttgtc cacgcgtttt ttttgttcga aaatttccaa
       61 tatactccaa aaagaagaaa aagcaaaaac agctaataag gaggaaaata aaatatgttt
      121 tactataata gacatacaaa tacctacaac tcctattaac aaaaataacc accaacaacg
      181 tgttccaaaa taatactcac atttaattac gatgggatga aataatccaa taatacaaaa
      241 tgtacttata cctatcagta caccaattaa attattgtgc attaaaaagt ccataattta
      301 ctctttataa atccaatatt tggttgattt cttcttaaaa tcctcctcat cttttctcca
      361 atagtgtttg attttatcaa aagaatagtt atttttttgt aattcgatac gaataaaatc
      421 actaccacat accttatcaa acatattcca tctactacta ttttcattcg taaaaacctc
      481 aaattctgga tataactgtt ttacaacctg caatacataa aactgaacct ctgacagacg
      541 tgccgttgta taatttgtaa aaaacacttg tactcctttt agatttttat ctttaccata
      601 tccataatac ggcttataat gaattgggcg aaatacaact ccatttaaac gaagagcatt
      661 tagacttgat gatagttttt ctgcatcaaa tatttcatga gcaaaggtct gaaatggcac
      721 agtatatcct actccaatag acattattcc taactcgcct aaaatgcctg aggcaggata
      781 atatacagca gactgcatgt acggaatatg cggagaagtc aatacccaag gtaattctgt
      841 ttcattaaaa cgcatagatc gtttccagcc ctccattggt acaatatgta attcacattt
      901 agctcctaac atattttctt cattcaacat atacgccaac tctcctactg ttaatccata
      961 gatgtatgga attttaaatt gactcacaaa agaaataaac gagtcttccg tagtatttcc
     1021 ttcaattttt aatcctccta acggattagg ccggtctaac acatatacag gaatcttctt
     1081 ttcagctgca gcttgcatag caagccctag tgtgctaata aaagtatatg aacggcatcc
     1141 aatatcctga atatcatata ctaatgcatc tattccctgt agcatttctt gcgttggttt
     1201 gcgtgtattg ccatacaaag aatatacttt tacacccgtt tttgtatcta tcaaattact
     1261 taccttttcg cctgcaggaa tatctcccct tacgccatgt tctggggcat acaatgcaat
     1321 tagcttaaca ttagttgcct catgtaatat atccacagtg gatttaagat ttttatttac
     1381 tccagtggga ttcgttatca acccaactct tttaccactt aaaacatcaa agtttctctg
     1441 ctgcaataca tcaatacctg tttttactac ttgcgaaaat agtgtaaacg atacataaaa
     1501 aaacaatata catattttat acttcatctt tattgggtaa taaactgttg tttcttgcta
     1561 ataaactctc tttacacaat aatcctaaac gataaaaatc aaataaaact aataatggat
     1621 gatgagaatg aaaatttttt tcaagatttt ctttactcac atcatataaa aaagctccca
     1681 tagcacacaa tcgcatcttt tttatctttt caaatactct tatcaattta atttgttcaa
     1741 acatactcat tttatctgat gtagtgtata atgtatatag atttttaaca gcatttctag
     1801 atttttctaa gtagatatca gtcttatcaa tagatacttg aaataattcg ttatcaatat
     1861 gtataacagg taccttattc tcttttagtg caataccaaa ccatgtatct tcatgaccat
     1921 atcctttaat tgtttcatcg aattgtatac tcgcaaatac cttgctaggt atacagaaat
     1981 taaacgaact aaacgattta taaggaaact tatttcgttc aacagccaga ttttgttctc
     2041 tatttcttcc atatttccaa cgaaataact gctgtgtatc cactggcggt tgaagcgaat
     2101 atttatatcc gccacaaatt acgccgtttt gataggaatt tgattgaaga cattgaatat
     2161 aattatgtaa ataataatcg cttgctggat atgtatcgca gtccataaat agaagatagt
     2221 catattttgc aatagtagct aatttgttgc gaatagcagc acgaccgata ttatttggta
     2281 attctatata cctatgattt gtattgttta gtgctttcct attttcttct ttataaacta
     2341 ttgaagaatc gtcaattgca actatctcaa caggaacatc aagctgatct atttgatgtt
     2401 gcaaagcata aattaatttg ctacaatttt ggttataaat tggtattaat acagaaagca
     2461 tacaaaaatg agtattacta aaaatggttc ataaagataa gaagaaaatt actatttgta
     2521 tacatgaata aaaaaaaagt gaagctccct tgatttggaa acttcacttt tttaactgac
     2581 tcaacttttt ctattctatt tcaatcatta aaaagttttt tggtatcttc tctcctacat
     2641 ttacgtttac agattttata ataccatcaa atggcatgtc tattctgttt tgcattttca
     2701 ttgcctccaa acgtaacaat aattcgcctt gttttacctt ttgaccaggt tttacattca
     2761 actcaataat tgttccagga atatacgatt ttatattatt tggattaggg gcttcccaaa
     2821 cttttctgtt tttaaatttt tttgttagca aagttttata tttacgtgca gttaccgcaa
     2881 aatctacaaa ttcactgtcc ttagttgctt ctgtcataac taactatttt ttgttactaa
     2941 ataatactac ttaagctatg aaatataata tgagtacaca ttaaaatgga ggtattccat
     3001 gtttcttttc aggacgataa tcatctttat ctttggatac cgaaagtgta tgtattaata
     3061 agttacgtgt ttcattaggc tcaattacag catctatata tcctttagca gctgcaacga
     3121 atggatttgc aaatttatca acatactctt gtactttagc agcacgcata gcatc
//
LOCUS       DAOPUR010000150         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_31677_length_3146_cov_3.297315, whole genome shotgun sequence.
ACCESSION   DAOPUR010000150 DAOPUR010000000
VERSION     DAOPUR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31677_length_3146_cov_3.297315"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKN90_03500"
     CDS             complement(<1..296)
                     /locus_tag="PKN90_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNZ85378.1"
                     /translation="MAIKFQYNKTSLQHLDKQLKVRKRALPTLKNKESALRSEVKRAK
                     DKAAALEEEFDKEWNKYTYMVKLWGEFDQSLIRVEDVVLSSKKIAGVLTPLLDEV"
     gene            complement(497..796)
                     /locus_tag="PKN90_03505"
     CDS             complement(497..796)
                     /locus_tag="PKN90_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85379.1"
                     /translation="MNPIDNEISSNLILNTILSPEQCGANGHFDKSREGKVAIISIDS
                     IDFISYKDDKILKRYRREGIYSTPSSTPTPFDIRQYKSSCCDYSSYDITYEDFDY"
     gene            complement(976..2295)
                     /locus_tag="PKN90_03510"
     CDS             complement(976..2295)
                     /locus_tag="PKN90_03510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNZ85380.1"
                     /translation="MERQAFQKIYTHLTNITKATVTLEAGDVGYEEMAIVHGRLAQVV
                     KIMGKMITLQVFSGTEGIPTDAEILFLGRPPQLKVSDELSGRFFNAYGDPIDGGPEIN
                     GEIRDIGSPSVNPVRRKQPSQLIATGIAGIDLNNSLVSGQKIPFFADPDQPYNQVMAS
                     VALRAKTDKIILGGMGLTNDDYLYFKNVFENAGALEKIISFVNTTDNPPVERLLIPDM
                     ALTAAEYFAVDKNESVLVLLTDMTLFADALSIVSNRMDQIPSKDSMPGSLYSDLARIY
                     EKAVQFPSGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDTEIGKVIVDPFR
                     SLSRLKQLVIGKQTREDHPQVMNAAIRLYADASNARTKMENGFDLTDYDERTLEYAKA
                     YSEKLLAIDVNVDTETMLSISWDLFSTHFSKAEVGIKAAITEKYWKN"
     gene            complement(2309..>3146)
                     /locus_tag="PKN90_03515"
     CDS             complement(2309..>3146)
                     /locus_tag="PKN90_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNZ85381.1"
                     /translation="SVYTAMTLAEYYRSMGLKILLMADSTSRWAQALREMSNRLEELP
                     GPDAFPMDLSAVISNFYARAGFVYLPNGTTGSITFIGTVSPAGGNLKEPVTESTRKAA
                     RCFYGLSQARADSKRYPAIDAIDSYSKYLEYPEVQTYLEKNLGKEWLGQVLQAKDILL
                     RGKEAMEQINILGDDSVPLVFHDKYWKSELIDFIILQQDAFDKIDASCPMNRQQYMLT
                     SVLKVANTEFKFDGFEEIGGFFKSIINIYKQMNYSEFQSEKFTQYESELNTLVSNKQV
                     NE"
BASE COUNT          903 a          588 c          544 g         1111 t
ORIGIN      
        1 acttcatcta acaaaggcgt gagtacaccc gcaatttttt ttgaggacaa taccacatct
       61 tctactcgaa tcaatgattg gtcaaattca ccccatagct ttaccatata ggtgtattta
      121 ttccattctt tgtcaaactc ttcttctaat gctgctgctt tatctttggc gcgttttact
      181 tcactacgca atgctgattc tttgtttttc agcgttggca gtgctctttt tcttaccttg
      241 agttgtttgt cgaggtgttg taaagaggtt ttattgtatt gaaattttat cgccataagt
      301 tcattattat ttacaatgga gtgtgctatg tatatttaac attcttaatt aagtattatc
      361 actttgtttc catgcattgc tagagcgaaa gttagataaa cattatgcga ttcgttatta
      421 ttatacagag ctaattttct taacttcatt aggaataata cataaagcat ttttaataca
      481 cacatatcat tatttattaa taatcgaaat cttcgtaggt aatatcgtac gatgaataat
      541 cacaacaact acttttatat tgcctaatat caaatggggt aggagttgag gatggcgtac
      601 tatatattcc ttctcttcta taacgtttta gtattttatc atctttatat gaaataaaat
      661 caatagagtc aatagaaatg attgcaactt taccttctct agacttatca aaatgcccat
      721 ttgctccgca ttgctccgga cttagaattg tattaagaat taaattactt gatatttcat
      781 tgtctattgg attcaaatat cttctaacaa taattacatc attagtacca tttctaaagc
      841 tataatcaaa ccgaaatgca gacggatctg ggtctcctcc tgaagtacag ttataaaata
      901 acagaacagt tacaaatata attaaattat ttagcttttt catatattgt aacaatttac
      961 ttatttgttt atttcttaat ttttccaata tttttcagta attgctgctt taatacctac
     1021 ttctgctttc gagaaatgtg tactaaataa atcccaagag atagaaagca tagtttctgt
     1081 atctacattt acatcaattg ccaatagttt ttctgaatag gctttggcat attctagcgt
     1141 acgttcgtca tagtctgtta ggtcaaatcc gttttccatt ttggtgcgtg catttgatgc
     1201 atctgcgtac aaacgaatgg ctgcattcat cacttgcgga tggtcttcgc gtgtttgttt
     1261 gccaataacc aattgtttta aacgtgataa tgagcggaat gggtcaacaa ttacttttcc
     1321 aatttcggta tcgcgacgca agaataattg tccttcggta atataccctg tgttatctgg
     1381 aatagcatgt gtaatatcgc caccagatag ggtggtaact gctataatgg taatggaacc
     1441 tcccgatgga aattgtactg ctttttcgta tatacgagct aagtctgaat acaacgaacc
     1501 gggcatactg tctttagatg gaatttgatc cattctattt gatacaatac ttaaagcatc
     1561 ggcaaacaag gtcatgtcgg taagtaacac caatacgctt tcgtttttat caactgcaaa
     1621 atactcggct gcagtaagag ccatatctgg tattaataag cgttctacag gagggttgtc
     1681 ggtagtattt acaaagctga tgattttttc taatgctcct gcattttcaa atacgttttt
     1741 aaaatacaaa tagtcatcgt tagtaagtcc catacctcct aaaataatct tatcggtttt
     1801 ggcacgcaac gctacgctag ccattacttg attgtaaggt tggtctgggt cagcaaaaaa
     1861 gggtattttt tgtcccgata ccaagctgtt atttaggtcg atacctgcaa ttcctgttgc
     1921 tattaattgc gatggttgtt ttctgcgaac ggggtttact gatgggctac caatatcgcg
     1981 tatttcgcca ttaatttcag gaccaccatc aatagggtcg ccataagcat taaaaaaacg
     2041 cccacttagt tcgtcgctta cttttagttg tggtggacga cctaaaaaaa gtatttcagc
     2101 atcggtaggt atcccttctg taccagaaaa tacttgcaag gtaatcatct tgcccataat
     2161 ttttactact tgagccaatc taccatgtac gatagccatt tcttcgtaac ctacatcgcc
     2221 agcttctaat gttactgtgg ctttggttat attggttaag tgggtatata ttttttgaaa
     2281 agcttgacgt tccatatttt atcttttctt attcgttaac ttgtttgttg cttacaagcg
     2341 tatttagttc cgattcgtat tgagtaaatt tttctgactg aaattcgcta taattcattt
     2401 gtttatatat attgatgatt gacttaaaga aacctccaat ttcttcaaaa ccatcaaatt
     2461 taaattcagt attcgcaact ttcaatacgc tagtaagcat atattgttgt ctgttcattg
     2521 ggcatgaggc atcaatttta tcaaaagcat cttgttgcaa aatgataaag tcaattagct
     2581 ctgatttcca atatttatca tggaatacca acggcacact atcatcgcct aagatgttga
     2641 tttgttccat agcttctttg ccacgaagca aaatatcctt ggcttgtaat acttgtccta
     2701 accactcttt ccctaagttt ttttccagat atgtttgaac ttcagggtat tcgaggtatt
     2761 tggagtaact atcaatagcg tcaatagccg ggtagcgttt actatcagca cgagcttgcg
     2821 ataagccata aaaacaacga gcagccttac gtgtgctttc tgtaacgggt tcttttaagt
     2881 ttcctcctgc gggtgataca gtgcctataa atgtaataga cccagtggtt ccgttaggta
     2941 aatacacaaa cccagcacgt gcatagaagt tggatattac tgctgataaa tccatcggaa
     3001 aagcatcagg tccaggaagt tcttctaatc ggtttgacat ttcgcgtagt gcctgagccc
     3061 aacgagaggt ggagtctgcc attaataata tttttaaacc catcgaacgg taatattctg
     3121 ccagcgtcat tgcggtatat acagac
//
LOCUS       DAOPUR010000151         3122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_32023_length_3122_cov_3.195631, whole genome shotgun sequence.
ACCESSION   DAOPUR010000151 DAOPUR010000000
VERSION     DAOPUR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32023_length_3122_cov_3.195631"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PKN90_03520"
     CDS             complement(<1..612)
                     /locus_tag="PKN90_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNZ85382.1"
                     /translation="MTNNWIVKELTTEESEHTEHLIKDLGISPILCKLLVQRGIHTME
                     EAKNFFRPQLSQLHDPFLLNDMDKAVARLNKAMRKKEKILIYGDYDVDGTTAVAVVYK
                     FLQQFYSSIDSYIPDRYEEGYGISYKGIDYAAENGFSLLIVLDCGIKAIEKVRYAKEK
                     GIDIIICDHHTPDEILPDAIAVLDPKRVDSTYPYPHLSGCGVGF"
     gene            805..1350
                     /locus_tag="PKN90_03525"
     CDS             805..1350
                     /locus_tag="PKN90_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNZ85383.1"
                     /translation="MIKQNNIVVKQYVDELRNAHIAFCYFDNKYGVLCDATNENLVHK
                     MYTKLLPQRMYLLVDNVVKLRHYVSEIPEIAYDLWETSENKVLIVFSELQNLSATIQQ
                     NSANVALGVLSDGFLQNLLQQFRIPILFIPFVSETDKEIAEIQHIIPYFNMVFDKNRK
                     LYAGYGIIKLQKDGTFVILRK"
     gene            1370..2785
                     /locus_tag="PKN90_03530"
     CDS             1370..2785
                     /locus_tag="PKN90_03530"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNZ85384.1"
                     /translation="MNHKKQVIKYVLADYITAVLAWFLFYVFRRIEIDLVAIRDIQMF
                     VPMYNARVTMLLVPFFWIVLYVVSGYYNTPFFKSRLSEFFTTILSVFFGSLIVFFSVL
                     MDDPVASYTFYYISFFVYFILHFSLTYAFRLLITTQSAHLIHTRKIGLRTLVLGVGEK
                     AFKLEKQLSEMNQSLGYIIEGFVALQKNETRKVNNSSVVGSIVDINNVLKKYSVDVVI
                     IAVDSITEDDIYQLMQSLWSKNIKIKIVPSLFEILTGSVKMNSIYAVPLIDLLEIQMS
                     DFQLNLKRLFDILLSSMVLLIGLPFFVIIALRIKLDSKGEVLYKQERVGLHGIPFMMY
                     KFRTMYENAEQDGVPRLSCENDERITPFGRFMRKYRIDELPQFYNVLKGDMAIVGPRP
                     ERRYFVNQIIEQAPYYYRLFNVRPGITSWGMVKYGYANTIEKMIERAQFDISYIENMS
                     LVIDSKILIYTIKTIVTGRGI"
     gene            2801..>3122
                     /locus_tag="PKN90_03535"
     CDS             2801..>3122
                     /locus_tag="PKN90_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNZ85385.1"
                     /translation="MKQTDIVNLWYLSLLKWRERKIKTSNFILVLSFLVGIFTALAAF
                     FLKNAIHFIQHILTTNFSVNHENYLYLAYPIIGIFITALFVRYVVKDDISHGVTRILY
                     AISKR"
BASE COUNT         1015 a          437 c          546 g         1124 t
ORIGIN      
        1 aaacccaaca ccgcaaccag ataaatgagg atatggatac gtagaatcaa ctcttttagg
       61 gtctaatact gcaatagcat caggaagaat ttcgtcagga gtatggtggt cgcaaataat
      121 aatatcaatc cccttttctt tggcataacg cactttctct atagccttaa tgccacagtc
      181 aagtacaatt aacagagaaa aaccattttc tgcagcataa tcaattcctt tgtatgaaat
      241 gccgtagcct tcctcgtatc tatctgggat atacgaatca atacttgaat aaaactgttg
      301 taaaaattta tataccacag ctactgctgt tgtaccatct acatcgtaat cgccatatat
      361 caagattttt tctttctttc tcattgcttt atttaagcgt gctactgctt tgtccatgtc
      421 gttgagtaag aaagggtcgt gtagctgact taattgaggt cggaaaaagt tttttgcttc
      481 ttccattgtg tgtatgcctc tttgtacgag tagtttgcac aaaattggac ttatccccaa
      541 atccttaatt aaatgttcag tgtgttcaga ttcttccgtt gtaagctctt ttacaatcca
      601 attgtttgtc atatagtgtg tttttattat cgctgtgata aaaactgtac aaaatcattg
      661 tatatttgta cgcataattc gattttcact tttttaaagt aaaaagaata aatcaaatcg
      721 taaagataca ataaaatagt aaaaacaact tcttgtgagt gtgaaaaaat gtttacaaga
      781 tatttttcga cttgaatgta aagaatgata aagcagaata atattgtggt taaacagtac
      841 gtagatgaat tgagaaatgc tcatatagct ttttgttatt ttgataataa atatggtgtg
      901 ctttgtgatg ctacgaacga aaatttagta cataaaatgt ataccaaact attaccccaa
      961 cgaatgtatt tattggtgga taatgtcgtt aaattaaggc attatgtgtc tgagatacct
     1021 gaaatagcgt atgacttgtg ggaaacatca gaaaataaag tattaattgt tttttctgaa
     1081 ttgcaaaatc taagtgcaac tattcaacaa aatagtgcta atgttgctct tggtgtactt
     1141 tcagatggat ttcttcaaaa tttattacaa caatttagaa ttcctatatt atttatccct
     1201 tttgtttcag aaaccgataa agaaattgca gaaatacaac atattattcc gtatttcaat
     1261 atggtatttg acaaaaatcg aaaattatat gcaggatatg gaataataaa attgcaaaaa
     1321 gacggaactt ttgtaatttt gcgaaagtaa tttaattaga ttatatatag tgaaccacaa
     1381 aaagcaagta atcaaatatg ttctcgcaga ctacataaca gctgtattag cgtggttttt
     1441 attctatgta tttcggcgta ttgaaattga tttagtggct attcgggata ttcaaatgtt
     1501 tgtacccatg tacaatgcac gtgttactat gttacttgtc cctttctttt ggatagtttt
     1561 atacgtagta tcaggatatt ataatactcc gttttttaaa tcgcgattgt cagaattctt
     1621 cactacaatt cttagtgttt tttttggtag cttgattgtg tttttttctg tattgatgga
     1681 cgatcctgtt gcctcataca cattttacta tatttctttt tttgtttatt ttatacttca
     1741 ttttagtcta acatatgcct ttcggctact tattactaca caatcagccc atcttattca
     1801 tacgcgaaaa ataggtttac ggacattggt attgggtgta ggagaaaaag cttttaaatt
     1861 agaaaagcaa ctttcggaaa tgaaccaatc tttaggttac ataatagaag gctttgtggc
     1921 attacaaaaa aatgaaacaa gaaaagttaa taatagcagt gtagttggct ctattgtgga
     1981 tatcaataac gttttaaaga aatattctgt tgatgttgtt attattgctg ttgatagtat
     2041 tactgaagat gatatttatc aattgatgca gtcgttgtgg tcgaaaaata ttaaaataaa
     2101 aattgtaccg agtttgtttg aaatattaac tggtagtgtg aaaatgaata gtatttatgc
     2161 tgtaccttta attgatttac tagaaataca aatgagcgat tttcagctca atttgaaacg
     2221 cttatttgat atcctccttt ctagtatggt tttgttgatt ggattgccat tttttgtaat
     2281 tattgcttta cgcattaaat tagattcaaa aggggaagta ttgtataagc aagagcgtgt
     2341 aggtttacat ggaattcctt ttatgatgta taagtttaga accatgtatg aaaatgcaga
     2401 acaggatggt gtaccacgtt tatcttgtga aaatgatgaa cggattactc catttggacg
     2461 ttttatgcgt aaatatagaa ttgatgagtt gcctcaattt tataatgttc tgaaaggaga
     2521 tatggctatt gtaggacctc gtccagaacg tagatatttt gtaaaccaaa ttatcgaaca
     2581 agctccgtat tattaccgtt tgttcaatgt gcgaccaggt attacatcgt ggggcatggt
     2641 aaaatatggt tatgccaaca ccatagaaaa aatgattgaa agggctcaat ttgatatctc
     2701 ttatattgag aatatgtcgt tagtaataga tagtaagata ttgatttata cgataaaaac
     2761 aattgtaaca ggaagaggaa tttgacgaat aaggaaagat atgaaacaaa cagacattgt
     2821 aaatttgtgg tatttatcat tgcttaaatg gcgagaaaga aaaattaaaa cgagtaactt
     2881 tatccttgtt ttgagttttt tggtaggaat ttttacagct ttagctgctt tttttctgaa
     2941 aaatgcaata cattttatcc aacatatatt aaccactaat tttagtgtta atcatgagaa
     3001 ttatttgtat ctagcgtatc ctatcattgg tatttttatt actgcacttt ttgtgcgtta
     3061 tgtagtgaag gatgatatca gtcatggcgt aacacgtatt ttgtacgcca tatccaaacg
     3121 ta
//
LOCUS       DAOPUR010000152         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_32051_length_3120_cov_3.376835, whole genome shotgun sequence.
ACCESSION   DAOPUR010000152 DAOPUR010000000
VERSION     DAOPUR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32051_length_3120_cov_3.376835"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKN90_03540"
     CDS             <1..863
                     /locus_tag="PKN90_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNZ85386.1"
                     /translation="SNWIREVHGSKSVTIDNMMEAYHHAASGNGFNEEFMWSQHECEL
                     TEKYGYITDKLHTDLHECVGHGSGKMLDGVSPEALKAYGATIEETRADLFGLYFIADP
                     MMVEIGLLPNMDAYKAEYYRFMLNGLMTQLVRIKLGDTIEESHMRNRQLIANWVFEKG
                     QKQKCIEKRVQNNKTYIIIHDYVALRTLFGELLAEIQRIKSEGDYTAAKYLVEKYAIQ
                     IDYSLHKEILERYTALDIAPYKGFVNPTYEQVYNEYGEVIDIVPHYTESYTEQMLRYS
                     KTKSYLPTYN"
     gene            995..>3120
                     /locus_tag="PKN90_03545"
     CDS             995..>3120
                     /locus_tag="PKN90_03545"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNZ85387.1"
                     /translation="MKLTFTIQYHTSWGQQVCILGSLPALGNNIPTKALKMNYIENGQ
                     WEASIQVRSLASFSYTYVVVDANGHTISEWGNPREIEIPASYKNAIIQDNWRSNGTDK
                     TFYSSAFTESFCARKQQTSFIPIDSPKTVRFSIHVPRIDSSLCLAIVGNQPALGNWDN
                     KKALVMQDTHYPEWTAEMDASNVKNPIEYKYVLYNRTKKTIVSWEKGANRTLEIDAVI
                     ANQLVLKNDETFRYDIPNFKAAGIALPIFSLRTENSFGIGEFADLKPLVDWSAKTGQK
                     VIQTLPINDTTRSRTNADSYPYSSITVLALHPAYINLIGIGKLKSKKEMDKFAQIQES
                     LKQFKTVKYQEVIEAKWEYFRLIYKQEKEKTFSSADYKQFFAENKQWLLPYAAFCYLR
                     DKHSDSNFRNWKNFSVFDWDKIDDFFKPEFADFDEAMIHIFLQYHAHKQLLEASNYAK
                     SKGIVLKGDIPIGISPDSVEAWMEPHLFNLDSQAGAPPDPFSATGQNWGFPTYNWERM
                     EKDGYQWWKTRFQKMAAYFQAYRIDHVLGFFRIWRMSADDVQGLLGHFDPALPLTADE
                     IKGFGTWFDYDRMVKPYIREHVLNGLFGDYTNEVKERFLDIEQYGVYRFKKAYNTQKK
                     IEAFFIKNQDKMEEHKIAILQNGLFALFCEVLFIEDSKQNGKFHPRIAFHSTYSYQDL
                     DGGAKNALNNLYNHFYYQKHNDFWKE"
BASE COUNT         1094 a          564 c          572 g          890 t
ORIGIN      
        1 actctaactg gatacgtgaa gtacatggat caaaatcagt aaccatcgac aatatgatgg
       61 aagcttatca tcacgctgct agtggtaatg gctttaacga agaatttatg tggagccaac
      121 acgaatgtga attgaccgaa aaatatggct atataaccga caaattacat accgacctgc
      181 acgaatgtgt aggacatggg tcaggtaaaa tgctcgatgg ggtttctcca gaagcgttaa
      241 aagcatacgg agcaacgata gaagaaaccc gagccgattt atttgggttg tattttattg
      301 cagaccctat gatggtagaa attggtttgt taccaaatat ggacgcatac aaagctgaat
      361 actatcgatt tatgcttaat ggattaatga ctcaactggt acgtataaag ctcggtgaca
      421 caatagaaga atcgcacatg cgcaaccgac aactcattgc taattgggtt ttcgaaaaag
      481 gacagaaaca aaaatgcatc gaaaaaagag tgcaaaataa taaaacctat atcatcatac
      541 acgattatgt agctttacgc acattgtttg gcgaactact tgccgaaatt caacgcataa
      601 aatccgaggg cgattacaca gcagccaaat acttagtaga aaaatacgct atccaaattg
      661 attactcttt gcacaaagaa attctagaac gttatactgc tctggatatt gcaccttata
      721 aaggatttgt aaatcccact tacgaacagg tgtataacga gtatggagaa gtaatagata
      781 tcgtacctca ttatactgaa agttatacag aacaaatgtt gcggtattca aaaactaaat
      841 cttacctacc aacatataac taaaatacct atcaaacgtt tgcaaggttt tttatgcatt
      901 tttgtagctc tatactagct ttggggatta caaacgtatt acatttgtct tcgttttacg
      961 ttaaactatt cctaactatt ttttgaaaaa aactatgaag ctaaccttta caattcagta
     1021 ccacacttcg tggggacagc aagtgtgcat acttggatca ctaccagctt tgggaaacaa
     1081 tattccaact aaagcattaa aaatgaacta catagaaaat ggacaatggg aagcaagcat
     1141 acaagttcgc tcgcttgcct ctttttctta cacatatgtg gtagtggatg caaatggaca
     1201 cacaatatca gaatggggaa atcccagaga aatagaaata ccagcttcat ataaaaatgc
     1261 aatcattcaa gacaattggc gttcgaatgg caccgataaa accttttatt cctctgcttt
     1321 tacggaaagt ttttgtgctc gtaaacaaca gacatctttt attcctatcg attcgcccaa
     1381 aacagttcga ttctctatcc atgtacctcg tatagactcc tcgctttgcc ttgctattgt
     1441 gggcaatcaa cctgctttag gtaattggga caacaaaaaa gccttagtaa tgcaagatac
     1501 ccattaccct gaatggacag cagaaatgga tgcgtcaaac gtaaagaatc ctattgaata
     1561 taaatacgtg ttatataatc gcacaaaaaa aacaatcgta tcgtgggaaa aaggtgccaa
     1621 tagaacattg gaaattgacg ctgtaattgc taatcaatta gtactaaaaa atgacgaaac
     1681 atttcgttac gatataccca actttaaagc ggctggtatc gcactaccca ttttctcgtt
     1741 acgcacagaa aatagctttg gaattggcga atttgctgat ttaaaacccc ttgtcgattg
     1801 gtcagctaaa acaggacaaa aagtcattca aacgctccca attaatgata caacacgttc
     1861 gcgtaccaat gctgattcgt atccatatag tagtattacg gtattggctt tacatcctgc
     1921 ttacatcaac cttataggta ttggcaagct gaaaagcaaa aaagaaatgg ataagtttgc
     1981 acaaatacaa gaatctttaa agcaatttaa aaccgttaaa taccaagaag ttatagaagc
     2041 taaatgggaa tattttcgtt taatatacaa acaagaaaaa gaaaaaacat ttagctcagc
     2101 tgactataaa caattttttg ccgaaaataa acaatggttg ctcccatatg cagcattttg
     2161 ttatttaaga gataaacaca gtgattcgaa tttcagaaac tggaaaaact tttctgtgtt
     2221 tgattgggac aaaatagatg atttctttaa accagaattt gccgattttg acgaggcaat
     2281 gattcatatc tttttgcaat atcatgctca caaacaatta cttgaagcaa gtaattacgc
     2341 taaatcaaaa ggcattgtgc tgaaaggtga tattcctata ggtattagtc cagatagcgt
     2401 agaagcgtgg atggaaccac atttatttaa cttagatagc caagctggtg caccccccga
     2461 ccctttttca gccactggtc aaaactgggg tttcccaacc tataactggg aaagaatgga
     2521 aaaagacgga taccaatggt ggaaaactcg ttttcaaaaa atggcagctt attttcaagc
     2581 atatcgtatc gaccacgtgt taggtttttt ccgcatttgg cgaatgtcag ctgacgatgt
     2641 gcaaggtttg ttaggtcatt ttgacccagc attgccttta acagctgatg aaatcaaagg
     2701 ttttggaact tggttcgact acgacagaat ggtaaaacct tacattcgcg aacacgtatt
     2761 gaatggttta tttggcgatt ataccaacga agtaaaagag cgttttttag acatagaaca
     2821 atatggtgtg tatcgtttca aaaaagcata taatacacaa aagaaaattg aagcattttt
     2881 tatcaaaaat caggataaaa tggaggagca taaaatagct attttgcaaa atggcttgtt
     2941 tgctttgttt tgcgaggttt tgtttatcga agatagcaaa caaaatggaa aatttcatcc
     3001 acgtattgct tttcatagca cctattcata tcaagattta gatggtggag caaaaaatgc
     3061 cttgaataat ttgtacaacc atttttatta ccaaaaacac aacgattttt ggaaagaaca
//
LOCUS       DAOPUR010000153         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_32558_length_3085_cov_2.770627, whole genome shotgun sequence.
ACCESSION   DAOPUR010000153 DAOPUR010000000
VERSION     DAOPUR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32558_length_3085_cov_2.770627"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKN90_03550"
     CDS             <1..186
                     /locus_tag="PKN90_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNZ85388.1"
                     /translation="LSGGYIFADNTLWDGKVVEPLVPNDKQTAGVLAFNRYIAADTRV
                     EKVVLPLRDGLTLIRKK"
     gene            235..570
                     /gene="rbfA"
                     /locus_tag="PKN90_03555"
     CDS             235..570
                     /gene="rbfA"
                     /locus_tag="PKN90_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNZ85389.1"
                     /translation="METKRQNKIARLIQKEISEILQSITKEMNQGVLITPTIVRVSPD
                     LGVAKIYLSIFPSEKSADMLAFVTERSQTIKHHLAGRTRFQLRTVPSLSFYLDDSLDY
                     IEHIDKLLK"
     gene            580..1800
                     /locus_tag="PKN90_03560"
     CDS             580..1800
                     /locus_tag="PKN90_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ85390.1"
                     /translation="MNFPLYIAKRYLFSKKSHNAINIISLISASGVAVGTVALVCVLS
                     VFNGFQGVIEGLFGKFDAQLQVTVNEGKSFNPQISSVQSLYKMPEIKVICEVVQDNAL
                     LIFSDKQTPAVIKGVEENYTQLNAMDEILLDGEFKLYDGVFDMGVAGVGLAQTLGCGV
                     RFVEPLQLFAPKRNTTVSLARPDNAFTSEFVFLSGIFMVQQAKYDANLLFVRLPLARK
                     LFGYDNEVTFLELRLTDGANELQVQKKIQTVLGDTFSVKNKYEQQADFFRMMEVEKWI
                     TYLILSFIVLIAIFNVVGSLSMLIIEKKNDTKVLMNLGANRKLIFKIFLFEGWLISIL
                     GVVVGILLGLVVCWIQIQFGILSLNGTDGAFIVEAYPVLVKWTDIVLIFGTVSLLGFL
                     AAIYPAHKLTREVK"
     gene            1833..2039
                     /locus_tag="PKN90_03565"
     CDS             1833..2039
                     /locus_tag="PKN90_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85391.1"
                     /translation="MKKLVFIFVSALFYSVSIVAQDDSVLEYVHLFPNCVDNQESTIY
                     GGTYGEYPPEIEDAIFPGGGFVKM"
     gene            2064..2369
                     /locus_tag="PKN90_03570"
     CDS             2064..2369
                     /locus_tag="PKN90_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNZ85392.1"
                     /translation="MQPAYTGEINENGDSLLVTGIVRVEFVVDRCGKVGRVRVVESVS
                     ELQDAEALRIVEGFPIFKPASLKDYRVKIAYIAPVYFSKKVMPRKAPEIDYDSYYYW"
     gene            2372..2857
                     /locus_tag="PKN90_03575"
     CDS             2372..2857
                     /locus_tag="PKN90_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNZ85393.1"
                     /translation="MIDIKELSLLAKKAEASSKHLFRKHKKIVEKLDDHVAVLHEKYT
                     AQYDCLACGNCCRSLGPFLTDRDIDKIAKALHLKPSAVVEKYIRIDEDGDYVFKTMPC
                     PFLMPDNYCQIYENRPKACKEYPHTDRKKFYQITNLSIKNSHVCPIVYEVLEDLKKEL
                     K"
     gene            2854..>3085
                     /locus_tag="PKN90_03580"
     CDS             2854..>3085
                     /locus_tag="PKN90_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNZ85394.1"
                     /translation="MIAIGDTIVSFDVINEQFLCDIASCKGACCIEGDCGAPLTLDEV
                     GILEEILPVVWNDLSEAAKKVIDKQGVAYADIE"
BASE COUNT          997 a          442 c          664 g          982 t
ORIGIN      
        1 ctatctggcg gttatatttt tgcagataac acattgtggg atggtaaggt tgtagaacct
       61 ttagtgccca atgataaaca aacagcaggc gtattagcat ttaataggta tattgcagca
      121 gatacgcgtg tagaaaaagt ggtattgccc ttgcgtgatg gattaacctt aatacgaaaa
      181 aaataacttc cagaaaaata tcgtatattt gcatagaata aagtaataac atgtatggaa
      241 acaaaaagac aaaataaaat agctcgactg attcaaaagg aaataagtga aattttgcag
      301 tcgattacga aagagatgaa tcaaggggta ttgattactc caactattgt gcgtgtgagt
      361 ccagatttgg gcgtagcaaa aatttatctg agtatatttc cttctgaaaa aagtgccgat
      421 atgttagcat ttgttacaga aagaagccaa accattaaac atcatttggc tggtcgtact
      481 cggtttcagt tacgtactgt acctagttta tctttttacc ttgatgattc gcttgactat
      541 atcgaacata tcgataaact tctaaaataa ggaatagccg tgaattttcc tttatacata
      601 gctaagaggt atttgttttc aaaaaaatcg cacaatgcca ttaatattat atcgcttatt
      661 tcagcaagtg gagtagctgt aggtactgta gcattggtct gtgtattgtc tgtttttaac
      721 ggatttcaag gagtgataga aggattgttt ggtaagtttg atgcacagtt gcaagttacc
      781 gtgaatgagg ggaaaagttt caatccacaa atttcttctg tacaatcttt gtacaagatg
      841 cccgaaataa aagtaatatg tgaggtggtt caggataatg ccttgctcat tttttctgat
      901 aaacaaacac cagctgtaat caaaggggtg gaagaaaatt acactcaact caatgctatg
      961 gatgaaatcc tgttagatgg tgaatttaaa ttgtatgatg gcgtatttga tatgggcgtg
     1021 gcgggtgttg gattagcaca aactttgggt tgtggagtgc gttttgtaga accattacag
     1081 ttgtttgccc ccaaacgaaa tactactgtc agcttagctc gaccagataa tgcgtttacg
     1141 tctgaatttg tttttttatc tggtattttt atggtgcaac aagccaaata tgacgctaat
     1201 ttattgtttg taaggttgcc attggcacgt aaattattcg gttatgataa tgaggttacc
     1261 tttttagaac ttaggctaac tgatggtgca aatgagttac aagtacaaaa aaaaatacaa
     1321 actgtattgg gtgatacttt ttctgtaaaa aataaatacg agcagcaagc tgattttttc
     1381 agaatgatgg aagtagaaaa atggattacc tatttaattt tatcttttat tgtgctaata
     1441 gctattttta atgtagttgg gtctctctcg atgttaatta tcgaaaagaa aaatgatacc
     1501 aaagtattaa tgaatttagg agcaaatcga aagttgattt ttaaaatatt tttatttgaa
     1561 ggatggctta tatctatact tggggtagtt gtgggtattc tactcggatt agttgtatgt
     1621 tggattcaga tacagtttgg catattatcg ttgaatggta cagatggagc atttattgta
     1681 gaagcatatc ctgtgttggt aaagtggaca gatatcgtgt taatatttgg tacagttagc
     1741 cttttaggtt ttttggctgc catttatcct gcacataagt tgacaagaga agtaaaataa
     1801 aggattaata tgtgtaaaca tgagtataaa atatgaaaaa gcttgttttt atttttgtta
     1861 gtgcactatt ctattctgtc tcaatagtag cacaagatga ttctgtgttg gagtatgtac
     1921 atttattccc caactgtgta gataatcaag aatctaccat ttatggtggt acgtatggcg
     1981 agtatcctcc tgaaatagaa gacgctattt ttccaggagg tgggtttgta aagatgtaac
     2041 agtatattta tacaaacacc gaaatgcaac ccgcatatac cggcgaaata aacgaaaatg
     2101 gagactccct gttggttacg ggtatcgtga gagtagaatt tgtagtagat aggtgtggta
     2161 aggtagggcg tgtgcgtgtg gtagagtctg tatccgaact gcaagatgca gaagctttgc
     2221 gaatagtcga aggttttccg atttttaagc ctgcaagttt aaaagattac cgagttaaaa
     2281 ttgcgtatat agcccctgtg tattttagca agaaagtaat gccacgcaaa gcccccgaaa
     2341 tagattatga tagttattat tattggtaag catgattgat ataaaagaat tgtccttatt
     2401 ggcaaaaaaa gccgaagctt cgtcgaaaca tctttttaga aaacacaaaa agattgtaga
     2461 aaaacttgat gatcatgtgg cagttttgca cgaaaaatat acagcacagt acgattgttt
     2521 agcttgtggt aattgctgtc gtagtttagg accttttctt acggataggg atattgacaa
     2581 gatagccaaa gccttgcact taaaaccatc ggcagtggta gaaaagtata tccgtattga
     2641 tgaagatggc gactatgtgt ttaaaaccat gccttgtcca tttttgatgc cagataatta
     2701 ttgtcagatt tacgaaaaca gacccaaagc ttgtaaggaa tatccacata ctgacagaaa
     2761 aaagttttac caaataacca atcttagtat taaaaatagc cacgtttgcc ccattgtgta
     2821 cgaagtgttg gaagacttaa agaaagaatt aaaatgatag cgataggaga taccattgtt
     2881 agttttgatg tcataaacga acaatttttg tgcgatatag cctcttgcaa aggagcttgt
     2941 tgtatcgaag gtgattgcgg tgcacccctt actctcgacg aagtaggtat tttagaggaa
     3001 atattgcctg tggtgtggaa cgatttatca gaagcagcca aaaaagtaat cgacaaacaa
     3061 ggtgtagcgt atgctgacat agaag
//
LOCUS       DAOPUR010000154         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_32726_length_3074_cov_2.912223, whole genome shotgun sequence.
ACCESSION   DAOPUR010000154 DAOPUR010000000
VERSION     DAOPUR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32726_length_3074_cov_2.912223"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..202)
                     /locus_tag="PKN90_03585"
     CDS             complement(5..202)
                     /locus_tag="PKN90_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85395.1"
                     /translation="MQVSKQQYHLSVSQWEPYSDHQLLSPFEALQAVIQYLNNNGLTE
                     IRFSTNIIILPSSNLRVFKIK"
     gene            complement(212..1066)
                     /locus_tag="PKN90_03590"
     CDS             complement(212..1066)
                     /locus_tag="PKN90_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNZ85396.1"
                     /translation="MNDVVKDIQISQYDYDLPDIRIAKYPLSQRDSSKLLVYKQGKID
                     QTVFSSISAHLPPRSLLVYNNTKVIHARLFFQKDTGAIIEIFCLEPIQPSDYQMVFQS
                     TKRCTWKCMVGNLKKWKQGALTLVCDVKGVSIELVAERDMIDSQIHIHFSWNNVGVTF
                     GDILEAIGKLPIPPYLHRESEENDEITYHTVYSKEQGSVAAPTAGLHFTDEVLARLHQ
                     TGICTTEITLHVGAGTFLPVKSATIGEHTMHTEMVVISKQTIKHLQASIGNIIAVGTT
                     SVRSLESL"
     gene            1194..1688
                     /locus_tag="PKN90_03595"
     CDS             1194..1688
                     /locus_tag="PKN90_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ85397.1"
                     /translation="MTRIGLLSDTHSYWDDAYLRHFKDCDEIWHAGDIGSISIIEQLA
                     STGKKIRAVYGNIDGQDVRGQFPLHNRFTVEEVTVWMTHIGGYPAKYNPNIIAELTKK
                     PPMLFVCGHSHIAKVMYDKSLQMLHINPGAAGKQGFHAVRTLMRFVVDKEVMKDLEVI
                     ELSQ"
     gene            complement(1677..2978)
                     /locus_tag="PKN90_03600"
     CDS             complement(1677..2978)
                     /locus_tag="PKN90_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNZ85398.1"
                     /translation="MKKRFIVLIVGLLSYFVVSAQFSVTGVDVVEYPQSDIAAVFVVD
                     AFTDGVLSYTLPENKDVTWSYYDESGNKTIIKSEIQVNKTSIPLQNNSSLGYELDIQG
                     ELQYVWVFHYPDFEVQLDDIQIDTNTEDRCQFIQAQLFITANSMKYQGYNQQTMPYEV
                     VRTFELAYDSAYFSQTTYEVESVVRKIEQTSYVQLTSPLMNTLYTLSGDQFARAFNRT
                     KEISSAQVEAFAVQINPTASIQTRDGLNEKERDLSSSQTLRGSAPLEVEVLSYANVPA
                     ARYFDWCLSREADFSTCELQYIDKDFRYSFTQQIPYYVRLNVYNADATCKADTSYVID
                     IVDSFLEIPNVFTPNGDGRNDEFRVAYKSLIKFSGKVYDSWGRLVFSWTDPTKGWDGT
                     INGLKAPEGAYFYHIEATGVDRDEKGNLIEYKKTGDINLLR"
BASE COUNT         1031 a          566 c          513 g          964 t
ORIGIN      
        1 taaattattt aattttaaaa accctcaaat tagaagaagg taaaataata atattcgttg
       61 aaaaacgaat ttctgttaat ccattattat tcagatactg tattactgct tgtaaagcct
      121 caaaaggaga tagtaactga tgatcagaat aaggttccca ttgagaaaca gatagatgat
      181 actgttgttt tgatacttgc acacctatat gctacaatga ctccaacgaa cggacactag
      241 ttgtaccaac agcaataata ttaccaatag atgcttggag gtgtttgata gtttgtttgc
      301 taataaccac catttcagta tgcatggtat gttctccaat agtagccgac ttgactggta
      361 aaaaagtgcc agcacctaca tgcaacgtaa tttctgtagt acagatacct gtttgatgca
      421 agcgtgctag tacctcatct gtaaaatgca aaccagccgt aggtgcagcc acagaacctt
      481 gctctttgga atataccgtg tgatacgtta tttcatcatt ttcttccgat tcacgatgca
      541 aataaggagg tataggcaat tttcctatgg cttcgagtat atcaccaaat gttacaccaa
      601 cattattcca cgaaaaatgt atatgtattt gcgaatcaat catatcacgt tcggcaacta
      661 gctctatgga aacacctttc acatcacata ccaaggttaa agctccttgt ttccactttt
      721 ttaggtttcc taccatacat ttccatgtac aacgttttgt tgattgaaaa accatttgat
      781 aatcagaagg ttgtataggc tccaaacaaa aaatctcaat gatagctccc gtatctttct
      841 gaaaaaataa acgagcatga attacctttg tattattata taccaacaaa ctacgtggag
      901 gtaaatgtgc agagatagaa gaaaatactg tttgatcaat ttttccctgt ttgtatacta
      961 ataatttaga agaatctcgt tggcttaacg gatatttagc tatacgtata tcaggtaaat
     1021 cgtaatcata ttgggaaatt tggatatctt ttacaacgtc attcatggat aaaattttgg
     1081 tacaaaaata caaaaatagc tagatatata ggaataataa cgcattttga aaaattaaaa
     1141 acaagtcatt tataaaattt acctatcttt acgtacttaa aattttgttc aacatgacac
     1201 gtattggttt actatcggac acgcatagtt attgggacga tgcataccta agacacttca
     1261 aagattgtga tgaaatatgg catgcgggag atattggtag tataagcatt attgaacaac
     1321 tagcatcaac gggaaagaaa ataagagccg tttatggaaa tatcgacgga caggatgtac
     1381 gtggacaatt tccattgcac aaccgtttta ctgtcgaaga agtaactgta tggatgacgc
     1441 atattggcgg atatcctgca aaatacaacc cgaacattat agctgaactt accaaaaaac
     1501 ctcctatgct atttgtatgt ggacattcgc acatagcgaa agtaatgtat gataaatctt
     1561 tgcagatgct acacattaat ccaggtgctg caggtaaaca agggtttcat gcagtacgta
     1621 cccttatgcg gtttgttgta gataaagaag tgatgaaaga tttagaagta attgaattat
     1681 cgcaataaat tgatatcccc ggtcttttta tattcaatca gattgccttt ttcatcccta
     1741 tctactccag ttgcttcgat atgatagaaa taagcccctt caggagcttt caatccattt
     1801 atagtaccgt cccagccttt agtaggatct gtccaagaaa aaactaaacg tccccaacta
     1861 tcgtatactt tgccactaaa ctttatcaat gatttgtaag ctactctaaa ttcatcattt
     1921 cgtccatctc cattaggcgt aaacacattg ggtatttcta aaaaagaatc cacaatatct
     1981 atcacataac tcgtatcagc tttacatgta gcatctgcat tgtatacatt taaccgtaca
     2041 taatacggta tttgttgggt aaacgaatac cgaaaatctt tatcaatata ctgtaattcg
     2101 caggtagaaa aatctgcctc acgcgagagg caccaatcaa aataacgtgc agcaggcaca
     2161 tttgcatagc ttagtacttc cacttccaaa ggagcagacc cacgcaaagt ttgcgatgaa
     2221 gacaagtccc tttctttttc atttaatcca tcacgcgttt ggatacttgc agttgggtta
     2281 atttgtactg caaatgcctc tacctgagca gatgaaattt ctttggtacg attaaatgca
     2341 cgtgcaaatt gatcaccaga caatgtataa agcgtattca taagaggaga cgtaagttga
     2401 acgtacgaag tttgttctat ttttcttacg actgactcta cctcataagt agtttgggaa
     2461 aaataagcag aatcgtatgc tagttcaaat gttcttacta cttcgtatgg catagtttgt
     2521 tgattgtacc cttgatactt catagaattt gccgtgataa acaactgtgc ctgaataaat
     2581 tgacacctat cctctgtatt agtatctatc tgtatatcat ccaattgaac ttcaaaatcg
     2641 ggataatgaa atacccaaac atactgtaat tcgccctgaa tgtcaagttc gtagccaaga
     2701 gatgagttat tttgtaacgg aatggaagtt ttatttactt gtatttcgga tttaataata
     2761 gttttatttc cagattcatc gtaataactc catgtaacat ctttgttctc aggcaatgta
     2821 taggataata caccatcagt aaatgcatct acaacaaata cagctgcaat atcagactga
     2881 ggatactcca ctacatcaac accagtaaca ctaaattggg ctgaaacaac aaaatacgaa
     2941 agcaaaccaa caataagtac aataaacctc tttttcattt tataattgct tagttatgtt
     3001 tgaaaaaagc gagcatttac tctttatttg taaaaagtca aaataatatg tcgttattat
     3061 tttgttaaac tgat
//
LOCUS       DAOPUR010000155         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_32775_length_3070_cov_2.577778, whole genome shotgun sequence.
ACCESSION   DAOPUR010000155 DAOPUR010000000
VERSION     DAOPUR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32775_length_3070_cov_2.577778"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..389
                     /locus_tag="PKN90_03605"
     CDS             30..389
                     /locus_tag="PKN90_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNZ85399.1"
                     /translation="MVGESKHNGERLQKAILLSKQLTIVIILKGAYTRVVLPNGKVYF
                     NTTGNPGMATAGSGDVLTGIIAGLRAQKYDAIEASLLGVYLHGLAGDYAKEKYSEKSL
                     VATDIIENISEAYKYIL"
     gene            complement(407..2353)
                     /locus_tag="PKN90_03610"
     CDS             complement(407..2353)
                     /locus_tag="PKN90_03610"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNZ85400.1"
                     /translation="MYNYVKVAAAVPNLEVANCYFNQESICAKIMEARHLGIEVLVFP
                     ELCITGYTCGDLFFQQTLQKATLDALQRILSATESIDMLVAVGAPLCIDNQLFNCAIV
                     LSKGKIVGIVPKTYIPNYNEFYEQRWFSSAFDLKINTINICSQVIPIGTDLLFSLHNG
                     TKIGVEICEDLWVPIPPSAGLCVSGADIILNLSASNETVAKRQYRRNLVVQQSARCMC
                     AYVYASAGGTESTTDLVFSGHSLIAENGVLLKENAQLIDSDYLLSSHVDIDKLRVDRC
                     KYTTYKETHRAYFKEIRCIPSDVQSKHNVSLDYPISRKPFVPSNLHLRDNRCQEIVAM
                     QVAGLVKRLVHTNAPTVVIGISGGLDSTLALLVCVQAFDELKKDRKGILAVTMPGFGT
                     TNRTYDNALTLIQELGVTRRVIDIKKSCYQHFEDIEHDETIHDITYENTQARERTKLL
                     MNLANKHRGFVIGTGDLSELALGWCTYNGDHMSMYAVNVSIPKTLIRSLVLNIAHTQE
                     EKIKQVLLDILDTPVSPELLPIGKNGEMIQKTEDSVGPYELHDFFLFYMMRYGFSPSK
                     ILFLAKHIFSKKETEEDVLFDDETIQKWLIVFYKRFFSQQFKRNCMPDGPKVGSICLS
                     PRGDWRMPSDAVAHEWLKEISGNL"
     gene            2559..>3070
                     /locus_tag="PKN90_03615"
     CDS             2559..>3070
                     /locus_tag="PKN90_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85401.1"
                     /translation="MKKIFTKKMTILFASVLFSGATAFATVDIEANETYTQNFDGIGT
                     EATATLPTGWKADKQTSVKKVGTYAAGVSATEYREGNNMSISAGNGIYNFGAGDKATA
                     TDRAVGGLSSKDNSKSVNFYVKLTNNGTTPISSFTISYDVEKYRMGKNPEEFAIEMYY
                     SANDETYTSAG"
BASE COUNT         1041 a          574 c          493 g          962 t
ORIGIN      
        1 tcactcctca ccctaagtaa tttgatagac tggtaggcga aagcaagcat aatggtgaaa
       61 gattacaaaa agccatcctt ctaagcaaac aattaactat tgttatcata ctgaaaggtg
      121 catataccag agtcgttctt cccaatggaa aagtgtattt taatacaaca ggaaacccag
      181 gtatggcaac tgcgggtagt ggagatgtgc ttacaggaat tatagcagga ttacgggcac
      241 aaaaatacga tgcaatagag gcatccttac tcggagtgta ccttcatggc ttggctgggg
      301 attacgctaa agagaaatat tccgaaaaat cacttgttgc aacagatatt attgagaaca
      361 taagcgaagc atacaaatat atactataga aaacagtatt cgcaaattat aaattacccg
      421 aaatttcttt taaccattcg tgtgcaacgg catcacttgg catacgccaa tcgcctctcg
      481 gagacaaaca aatactccct actttaggac catcaggcat acagttacgt ttaaattgtt
      541 gactaaaaaa ccgtttataa aacacaataa gccatttttg tatggtttca tcgtcaaata
      601 acacatcttc ctctgtttct tttttagaaa atatgtgctt tgcaagaaat aaaattttac
      661 ttggactaaa cccataacgc atcatataaa acaaaaagaa atcatgcaat tcgtaaggac
      721 ctacgctatc ttcggttttt tgtatcattt cgccattttt tcctattggt aataattctg
      781 gacttactgg agtgtctaat atgtctaaca aaacctgttt aatcttttcc tcttgtgtat
      841 gtgctatatt caacactaaa ctacgaatta acgttttggg tatacttaca ttcacagcat
      901 acatactcat atgatcacca ttgtacgtgc accaacccaa agcaagttcg ctcaaatcgc
      961 cagttccgat aacaaaacct ctatgcttat tcgctaaatt cattaacagt ttagttcgct
     1021 cccgtgcttg tgtattttcg taagtaatat catggatagt ctcatcatgc tctatgtctt
     1081 caaaatgctg ataacaagat tttttaatat caataactct acgtgtaact ccaagttcct
     1141 gaatcaaggt aagagcatta tcatacgttc tattcgtagt accaaaccct ggcatagtta
     1201 cagcaagtat accttttctg tcttttttca gttcatcaaa agcttgcaca cacaccaaaa
     1261 gagctaaagt agaatccaat ccccctgata tacctattac aacagttgga gcatttgtat
     1321 gaactaaacg tttgactaaa cctgctactt gcatagcaac tatttcttga caccgattat
     1381 cgcgtaggtg taaattacta ggtacaaaag gctttcggct aataggatag tcaagcgata
     1441 cattatgttt actttgaaca tcagatggta tgcacctaat ttctttaaaa taggctcgat
     1501 gggtttcttt gtaggttgta tacttgcatc tatctacacg taacttatct atatctacat
     1561 gtgaggaaag tagataatct gaatctatta attgtgcgtt ctcttttagc aaaacgccat
     1621 tttctgcaat caaagagtgc ccactaaaca ctaaatcagt tgttgattca gtccctccag
     1681 cactcgcata tacataggca cacatacaac gtgctgactg ctgaacgact aaatttcttc
     1741 tatattgtct tttggcaaca gtttcgttac tagccgataa attaagaata atatctgctc
     1801 cagacacaca aagacctgca ctaggaggaa ttggaaccca caaatcttca caaatttcaa
     1861 caccaatttt agtgccattg tgcaacgaaa ataacaaatc agtacctatt ggtattacct
     1921 gactacatat gtttattgtg tttattttca aatcaaatgc agatgaaaac caacgctgtt
     1981 cgtaaaattc attataattt gggatatacg ttttaggtac tattcctact atcttacctt
     2041 ttgacaaaac aatagcacaa ttaaaaagct gattatcaat acacaatgga gctcctacag
     2101 caacaagcat atctatactt tctgttgcag aaagtattcg ttgtaaagcg tctaaagtgg
     2161 ctttttgtag tgtctgttga aaaaataaat caccacaagt atatcctgtt atgcataatt
     2221 ctggaaaaac caatacctca atcccaagat gtcttgcttc cataattttt gcacaaattg
     2281 attcttgatt aaaataacaa tttgcaactt cgagattggg tactgcggct gctaccttta
     2341 cataattata catatagcta catatttagt tgttacaaaa gtataaaaaa tatctaatat
     2401 tgttgataag atacaaccta attgtctctt tgaattgtta agatataatg aattgttagg
     2461 tttttagtgg agatttattt tttattaaaa aaaaataacc ctatatttgt gattaatagg
     2521 aaacacataa attattcaca cattaaattt aaaacattat gaagaaaatt tttacaaaaa
     2581 aaatgaccat tttgtttgct tctgtcttat ttagcggagc aacagcattt gcaacagtcg
     2641 atattgaagc aaatgaaacg tatacgcaaa actttgatgg tataggaact gaagctacgg
     2701 ctactttacc tacaggatgg aaagccgata aacaaacatc cgttaaaaaa gtaggaacct
     2761 atgcagctgg tgttagtgca acagaatatc gagaaggtaa caatatgagt atctcagcag
     2821 gtaatgggat ttataacttt ggtgcaggtg ataaagcaac agcaactgat agagctgtag
     2881 gaggactatc ttctaaagat aattcaaaaa gtgtaaactt ctatgtaaaa cttactaata
     2941 atggaactac tcctatttct tcttttacta tcagctatga tgtagagaaa taccgtatgg
     3001 ggaaaaaccc tgaagaattt gctatcgaaa tgtactattc tgctaatgat gaaacatata
     3061 cttctgctgg
//
LOCUS       DAOPUR010000156         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_32862_length_3064_cov_3.380193, whole genome shotgun sequence.
ACCESSION   DAOPUR010000156 DAOPUR010000000
VERSION     DAOPUR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32862_length_3064_cov_3.380193"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..458)
                     /locus_tag="PKN90_03620"
     CDS             complement(15..458)
                     /locus_tag="PKN90_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ85402.1"
                     /translation="MSLFGIFSQAYAQVPLASHDVVLQGFMWNSHTTTKWSVLKPQAS
                     EIAESFTLVWLPPSAAAEGGGTSNVGYHPYKWSNQTSSWGSRAELEALITDLKAGGAK
                     VLADIVVNHRAGNGWCDGFPTDNFGEYGVFTLKASHITKDDEASG"
     gene            complement(691..2424)
                     /locus_tag="PKN90_03625"
     CDS             complement(691..2424)
                     /locus_tag="PKN90_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNZ85403.1"
                     /translation="MTKKTETVRLITFFKEIFYPKLFSSLRLYSKEKFLSDTMAGVIV
                     GIVAIPLAIAFGIASGVGPTEGLITAVIAGFIISFFGGSKVQIGGPTGAFIVIIYGII
                     QQHGLTGLLVATIMAGVMLVLMGVFKLGSIIKFVPYPVIVGFTGGIALTIFSTQINDF
                     FGLGISNLPADFIHKWISYFSHFTAIHWWSFIIALISFFIILVMPLFSKKIPGSLVAI
                     ILATILVYFLKLTSFAGVDGIKTIQDIYILPEGIPMPHLPSIALAENQTLWGLIQTLF
                     PAAFTIAMLGAIESLLSAMVADGVIRDKHNSNTELIAQGMANIVVPFFGGIPATGAIA
                     RTMTNINNGAKTPIAGIIHAIVLLLVLLFLGKFVGYIPMACLAAVLIIVAYHMSGWRS
                     FISLSKNPKSDFVVLMTTFLLTVIFDLTIAIEIGLLLAVVLFLKRTNEATIIRVFQHK
                     IDPAENSDILLQDDPLYIPKEVDVYEIDGPYFFGIATKFDEVMETVAEKPKVRIIRMR
                     KVSFIDSTGIHHLQNLCEQSHREKIVIILSGVNKHVRGALMKANFFSILPEERVCSTI
                     HEALELSEQYI"
     gene            complement(2534..2644)
                     /gene="rrf"
                     /locus_tag="PKN90_03630"
     rRNA            complement(2534..2644)
                     /gene="rrf"
                     /locus_tag="PKN90_03630"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2762..>3064)
                     /locus_tag="PKN90_03635"
     rRNA            complement(2762..>3064)
                     /locus_tag="PKN90_03635"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1107 a          607 c          455 g          895 t
ORIGIN      
        1 ccagaacatt ctgatcatcc tgaagcttcg tcatctttcg taatatgtga agcttttaaa
       61 gtaaaaacac catattcacc aaaattatct gttggaaaac catcacacca accattacct
      121 gcacgatgat ttaccacaat atcagctaat actttagctc cacctgcttt taaatctgta
      181 attaaggctt ctaactcagc acgtgaaccc cacgaactag tctgattaga ccatttgtaa
      241 gggtgatatc ctacattaga cgtcccgcca ccttcggcag ccgctgacgg tggtaaccat
      301 actaaagtaa acgactcagc tatttcagac gcttgaggtt ttagaacact ccatttagta
      361 gttgtatgtg aattccacat aaatccctga agcacaacat catgagaagc caaaggaacc
      421 tgagcgtatg cctgagaaaa tatgccaaaa agagacaaaa agacatacag taaaagtgat
      481 tttgtttttt cattgtataa aatattaagg tttaattaac ttttagacta aaaatttagc
      541 taaaaaaatg aattatccca taccttctcc acattttggt aagatttaac attcttgtaa
      601 taaacaatat tagcacttct atgtaatttt ctttttctac atgataacgt tgtgagactt
      661 tatatatctg tctaccttat aaaacatcta tcaaatgtat tgttcagata attctaaagc
      721 ttcatgaatt gtggaacaaa ctctttcttc tggtaatata ctaaaaaaat tggctttcat
      781 taatgctcct cgtacatgtt tattcacacc agacaaaatg attactattt tttccctatg
      841 cgattgttca cataaatttt gtaaatgatg aatgcccgta gaatcaataa aagaaacttt
      901 acgcatacga attatacgaa ctttgggttt ttctgccact gtttccatta cttcatcaaa
      961 tttggtagct ataccaaaaa aataaggtcc atcaatttca taaacatcaa cctctttggg
     1021 aatatataat gggtcgtctt gaagaagaat atcagaattc tcagcaggat caattttatg
     1081 ttgaaaaact cgtattatag tcgcttcgtt cgttctttta aggaataata ctactgctaa
     1141 taataatcct atttcaatcg ctatagttaa atcaaaaatt accgttagta aaaaagttgt
     1201 cattaaaacc acaaaatctg attttggatt ttttgataaa gatataaacg atctccatcc
     1261 actcatatga tatgccacaa taattaagac agcagctaaa catgccattg gaatatatcc
     1321 aacaaatttt cctaaaaata aaagaactaa caataaaaca atagcatgta taattcctgc
     1381 aataggggtt ttagcaccat tattgatatt agtcatcgtt ctagcaatag ctcctgtagc
     1441 aggtatacct ccaaaaaaag gaactacaat attcgccatc ccttgtgcaa tcaactccgt
     1501 attagaatta tgtttatcac gtataactcc atctgcaacc attgcagaaa gtaacgattc
     1561 aatagcccct aacatagcta tcgtaaaagc tgctgggaat aacgtttgta tcaatcccca
     1621 taacgtttga ttctctgcta atgcaattga tggcaaatga ggcattggaa taccttctgg
     1681 taaaatatag atatcttgga ttgttttaat tccgtctact cctgcaaatg atgttaattt
     1741 taagaaataa acaaggatag tagctaatat gattgcaacc aaagaaccag gtatcttttt
     1801 tgaaaacaaa ggcataacaa gaataatgaa gaaactaatt aatgcaatta taaaagacca
     1861 ccaatgtatt gctgtaaaat gggaaaaata agaaatccat ttatgaataa aatcagcagg
     1921 taaattagat attcctaaac caaaaaaatc attaatctga gtagaaaaaa ttgtaagtgc
     1981 aataccacca gtaaaaccaa caataacagg ataaggtaca aacttaataa tagaacctaa
     2041 tttaaaaacc cccatcaata ccaacattac accagccata atggtagcta ccaataatcc
     2101 cgtaagacca tgttgttgaa ttataccata aataattaca ataaatgctc ccgttggacc
     2161 tccaatctgc acttttgaac ctccaaaaaa tgatataata aacccagcaa taactgcagt
     2221 aataagtccc tcagtaggac cgactcctga tgcaatacca aatgcaatag ccagtggtat
     2281 agcaacaata cccactatta caccagccat agtgtcagac aaaaactttt ccttagaata
     2341 aagacgtaaa gaagaaaata attttggata aaagatttct ttaaaaaaag ttattaatct
     2401 tacagtttca gtttttttag tcataagtgt ataaaacgtt aaaatcagac aaattataga
     2461 ataaagaata gacctaataa attctaaaac gacaaaggag gagacgtata aaaacatctc
     2521 ctcctttggt aaaaaacggc ggctatctac tctcccacac aagtgcagta ccatcgacgc
     2581 tattgggctt aacttctctg ttcggaatgg gaagaggtgg aaccccaacg ctataaccac
     2641 ctaaaattag tgtagcgtat tgttcattga ttgctcaata cgtattatat acaaaataga
     2701 aaatacatca tcttaagaaa aaagaagaaa aaacaccatt catctaaatc aaggtatttt
     2761 gttgtaaaga aagttcgggc tattagtact gctcggctat gacatctctg cctttacacc
     2821 tgcagcctat caacgtagta gtcttctacg accctaatgg aaatctaatc ttgaggatgg
     2881 cttcgtgctt agatgctttc agcacttatc cgtaaccgaa cgtagctact cggcaatgca
     2941 cctggcggta caaccgataa accagcggtt cgtccaacac ggtcctctcg tactagtgtc
     3001 agaccctctc aaatttcctg cgcccacaac agatagggac cgaactgtct cacgacgttc
     3061 tgaa
//
LOCUS       DAOPUR010000157         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_33217_length_3042_cov_2.556411, whole genome shotgun sequence.
ACCESSION   DAOPUR010000157 DAOPUR010000000
VERSION     DAOPUR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33217_length_3042_cov_2.556411"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PKN90_03640"
     CDS             <1..1316
                     /locus_tag="PKN90_03640"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNZ85404.1"
                     /translation="IADAIQLGATAIVCEQLPTSIHSDIAYLQVADSAVTMGNIAANW
                     YDNPSQKLTLVGVTGTNGKTTIATLLYNLFEQLGYVSGLLSTVCNYVHKEQIPSTHTT
                     PDALSIQALLHRMVIAGCSYAFMEVSSHASDQKRTAGLHFAGGIFTNLTRDHIDYHQT
                     MEAYLKAKKTFFDLLPKTAFALTNTDDKNGLVMLQNTAANKYTYSTQTLADFKGKIIE
                     LSFEGMLLSINNKELSVAFVGKFNVSNLLAVYGTAVLLGIHPDEALVALSTLKPVSGR
                     FETLQSPTGFTAIVDYAHTPDALNNVLETINDIRSEGIAENMGKLYTVVGCGGNRDKG
                     KRPIMAKEAVKASNMAIFTSDNPRNENPKDILNDMVAGINEEEKKSTLVIEDRYQAIK
                     TACMLAQRGDIILVAGKGHETYQEIAGVKHHFDDKEVLAELFAQL"
     gene            1330..2556
                     /gene="mraY"
                     /locus_tag="PKN90_03645"
     CDS             1330..2556
                     /gene="mraY"
                     /locus_tag="PKN90_03645"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNZ85405.1"
                     /translation="MLYYLFRFLEENFNLPGAGLFSYVSFRAGMALIFSLFISMVIGK
                     NIINYLRKKQIGESIRDLGLDGQMSKKGTPTMGGIIIIIAILVPSLLFGRIRNIYLLL
                     MIATTVWLGVIGFLDDYIKVFRKHKEGLNGKFKIVGQIGIGLLVGMTMFLSPEVVMIE
                     NTNHVKVQHIETTELDSKSITVKSTQTTIPFIKNTNFDYADFLPFLKEHAQTGAWILF
                     IIITIFIVTAVSNGANLTDGLDGLAAGSSSIIGITLGVLAYVSSHVGFAGYFDIMFVP
                     GSEELVVFASAFVGATVGFLWYNSYPAQVFMGDTGSLTIGGIIGVFAILIHKEFLLPI
                     LCGIFLIENLSVMMQVSYFKYTKKKYGEGRRIFLMSPLHHHYQKKGIPEAKIVTRFWI
                     IGIALAIFTLITLKIR"
     gene            2559..>3042
                     /locus_tag="PKN90_03650"
     CDS             2559..>3042
                     /locus_tag="PKN90_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNZ85406.1"
                     /translation="MKKIVILGGGESGVGAAVLAKKQGFDVFVSDASGIKQKYREVLQ
                     KYAIRWEEGTHTYSEIMDATEVIKSPGIPDKAPLIKEIKARHIPIISEIEFAGRYTKA
                     KTICITGSNGKTTTTMLTYHILQQAGLNVGLAGNVGKSFALQVAENQFDYYVIELSSF
                     Q"
BASE COUNT         1057 a          515 c          588 g          882 t
ORIGIN      
        1 acattgcaga tgctattcaa ttaggagcca cagctattgt ttgcgaacaa ctaccaacat
       61 ccattcatag cgatattgca tacttacaag ttgctgatag tgctgtaact atgggtaata
      121 tagcagctaa ttggtatgat aatccttcac aaaaactaac tcttgtaggt gtaacaggta
      181 ccaatggaaa aacaactata gccactttgc tatataatct atttgaacaa ttaggctatg
      241 tttctggatt actttctaca gtatgtaatt atgttcacaa agagcaaata ccaagtacac
      301 acaccacgcc cgatgctctt agcatacaag ctttattaca tagaatggtt atagcaggat
      361 gttcgtatgc atttatggaa gtaagttcgc acgctagcga tcaaaaacgc actgctggat
      421 tacattttgc aggaggtatt tttacaaatc ttacacgcga ccatatcgat tatcatcaaa
      481 caatggaagc ctatttaaaa gccaaaaaaa cattttttga cttgttgcca aaaacggctt
      541 ttgcacttac aaatactgat gataaaaatg gattggtcat gttgcaaaat actgctgcaa
      601 acaaatacac ctattcaacc caaacattag ctgactttaa aggaaaaatc attgagctaa
      661 gctttgaggg catgttactt tctatcaata acaaagagct atcagtagca tttgtaggca
      721 aatttaatgt ttcaaattta ctagctgtat acggaacagc agtattactt ggcatacacc
      781 ccgacgaagc tctcgtcgct ttgagtacac taaaacctgt aagcggtcgt tttgaaacgc
      841 tacaatctcc tactggattt acagctattg tagattatgc acacacacct gatgcattga
      901 acaacgtatt agaaacaatc aatgatatcc gatcagaagg aatcgctgaa aatatgggaa
      961 aactatacac agttgttggt tgtggtggta atagagataa aggtaaaaga cccataatgg
     1021 caaaagaagc agtaaaggca agcaacatgg caatctttac ttcagataac cctcgcaatg
     1081 aaaaccccaa ggatattctg aatgatatgg tagcaggaat aaatgaagaa gaaaaaaaat
     1141 caacactagt aattgaagat aggtatcaag ccataaaaac agcctgtatg ttagctcaac
     1201 gtggagatat tattctggtg gcaggaaaag gacacgaaac gtatcaagaa atagcaggcg
     1261 taaaacatca ttttgacgac aaagaggtat tagcggaact atttgctcaa ctataaaaca
     1321 aagtagcgta tgttgtacta tttatttaga tttttagaag aaaactttaa cttacctggt
     1381 gcaggtttgt ttagttatgt atcattccgt gcaggcatgg cactcatttt ctcgctcttt
     1441 atttcgatgg ttatcggaaa aaatatcatc aactatttgc gtaaaaaaca aataggagaa
     1501 tccattcgtg atttagggct tgatggacaa atgagcaaaa aaggaacacc cactatggga
     1561 gggattatta tcattatagc catcttggtt cctagtttat tgtttggtcg catacgtaat
     1621 atatatttac ttttgatgat agcaactaca gtatggttgg gagttatcgg ttttctcgac
     1681 gactatatta aagtgttcag aaaacacaaa gagggattaa acggaaaatt caaaattgtt
     1741 gggcaaattg gcattggact attagtgggt atgaccatgt ttttaagtcc tgaagtagta
     1801 atgatagaaa ataccaatca cgttaaagta caacatatag aaacaaccga attagactca
     1861 aaaagcataa ctgtaaaatc gacacaaacc actattccat ttatcaaaaa tacaaacttt
     1921 gattatgctg attttttacc attcttaaaa gaacacgcac aaacaggagc atggatttta
     1981 tttattataa ttactatatt tattgtaaca gcagtgtcaa atggagcaaa cctaacggat
     2041 ggattagatg gacttgctgc tggttcgtct agtatcattg gtattacact gggcgtatta
     2101 gcatatgtat ctagccatgt ggggtttgct gggtattttg acatcatgtt tgtgcctgga
     2161 agcgaggaat tagtagtatt tgcaagtgct tttgtgggag ccacagttgg ttttttgtgg
     2221 tacaactcct accctgctca ggtatttatg ggcgacacag gaagtttaac aattggaggc
     2281 attattggcg tgtttgcgat actcatacac aaagaatttt tactaccaat cttatgtggc
     2341 attttcttaa tagaaaacct gtcggtaatg atgcaagtat cgtactttaa atatacaaaa
     2401 aagaaatatg gagaagggag acgcatattt ttaatgtccc ctttacatca tcattatcag
     2461 aaaaaaggca ttcctgaggc taaaattgta actcgttttt ggattattgg tattgcactg
     2521 gcaatattta cacttataac acttaaaatt cgctaaatat gaaaaagata gtgatacttg
     2581 gtggaggaga aagcggagta ggtgctgctg tattagcaaa aaaacaaggc tttgatgtat
     2641 ttgtttcaga cgcttctggt atcaaacaaa agtatcgaga agtattacaa aaatatgcta
     2701 tcagatggga agaaggaaca catacatact cagaaataat ggatgctaca gaggtgatta
     2761 aaagccctgg tataccagac aaagcccctc tcattaaaga aataaaagct agacacatac
     2821 ccattatttc ggagatagag tttgctggtc gttatacaaa agctaaaacc atttgtatca
     2881 caggaagcaa tggaaaaacc accaccacaa tgctaaccta tcacattttg caacaagcag
     2941 gattgaatgt gggactagca ggcaacgtgg gtaaaagctt tgcactacaa gtagcagaaa
     3001 atcagtttga ttattatgta attgaactaa gcagttttca ac
//
LOCUS       DAOPUR010000158         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_33246_length_3040_cov_2.956114, whole genome shotgun sequence.
ACCESSION   DAOPUR010000158 DAOPUR010000000
VERSION     DAOPUR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33246_length_3040_cov_2.956114"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PKN90_03655"
     CDS             <1..944
                     /locus_tag="PKN90_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNZ85407.1"
                     /translation="FNKTVYDANVNMLRTQTEAMSASLGGVDSLTVLPYDSVYKKSDD
                     FSERIARNQQLLLKEESNFDKVADPASGSYYIETLTNEIAQQAWKLFLEVEENGGFYA
                     AIVAGNVQKAIKKTASTRLKNVSSRREVLLGTNQFPNFNEQAITKVQKENNASSCCTH
                     STSIEQLCAVRAADEFESLRFATEKATKRPKAFMLTIGNLAMRLARAQFSCNFFACAG
                     YDVVDNLGFKTIEEGIAAAKKANADIVVLCSSDDEYAELAPQAFDLAKGNAILVVAGA
                     PACTDELKAYGIEHFINVKSNVLDTLRTFNGLLTINQ"
     gene            947..1846
                     /locus_tag="PKN90_03660"
     CDS             947..1846
                     /locus_tag="PKN90_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNZ85408.1"
                     /translation="MSPTTKNWIIATRPWSFPASAMPALFAMAYVFYMQDSIESIHWG
                     YGVAALLGAVLFQASGNLIGDYFDYMYKVDRKESLGSSRMLVDGVFTPKTIFWFGIIV
                     LCMGILLGLFLWSQTGNDLLWIGIVGALGTFFYYKLKYMALGDVLIFVIYGPLIALGT
                     AFVMTNQLYWEVVVLSIPIAFLVVNILHANNTRDMRDDKLANIKTQALLLGEKGSRIQ
                     YTFLAIGAYVAVAIFVLLNMISPLALLVIVTLPLAKKNINLIKQAKIDKPEIIKDLDA
                     MSAQLVMAFTLMYALGIFIGYWI"
     gene            1843..2457
                     /locus_tag="PKN90_03665"
     CDS             1843..2457
                     /locus_tag="PKN90_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNZ85409.1"
                     /translation="MKKVLLASLLAAVLWFIMFSPWTSIHINFWIAMACSAAILWAMS
                     AFLDKNFSKQFSFNVKDLVIGLMSAAVLYGVFVVGNYLSTQWFDFAGTQIQGIYGMKE
                     GQNLVLVGVLLTVLIGPAEEIFWRGYVQKQLGARYGDWFAVLITVLIYTLVHIWSFNF
                     MLIMAAMVCGLFWGILYKYHKNIVTLIVSHAVWDVAVFILIPIA"
     gene            2534..>3040
                     /locus_tag="PKN90_03670"
     CDS             2534..>3040
                     /locus_tag="PKN90_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNZ85410.1"
                     /translation="MKPDFKSIDITNIAVSSGVGTQAESTWVTPEQIPVKPFYTKQDL
                     NGMQHLQYAAGLPPYLRGPYSVMYAMQPWTIRQYAGFSTAEESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDADHERVVGDVGKAGVSICSIEDMKVLFDGIPLNKMSVSMTMNGAV
                     LPVLAFYIN"
BASE COUNT          909 a          459 c          649 g         1023 t
ORIGIN      
        1 aatttaacaa aacagtatat gatgccaatg taaatatgct tcgcacacaa acggaagcca
       61 tgagtgcttc gttgggtggg gtagattcac taaccgtatt gccttatgat tctgtgtaca
      121 aaaaatcaga tgatttttcg gagcgtattg ctcgtaacca acaattattg ttgaaagaag
      181 aatcaaattt tgataaggtg gctgacccag cttctggttc gtactatatc gaaacattaa
      241 ccaatgaaat agctcaacaa gcttggaaat tatttttaga agtagaagaa aatggtggtt
      301 tttatgcagc tattgtagca ggtaatgtac aaaaagcaat taaaaaaact gcaagtacgc
      361 gtttaaaaaa tgtttcatct cgccgtgaag tgttacttgg aaccaatcaa ttccctaatt
      421 tcaacgagca agcaattact aaagttcaaa aagaaaataa tgcatcatca tgttgcacac
      481 atagtacatc tatcgaacag ttatgtgcag tacgtgcagc tgatgaattt gaatccttac
      541 gttttgctac cgaaaaagca actaagcgtc caaaagcatt tatgctaacc attggtaact
      601 tggctatgcg tttggcaagg gctcagtttt cgtgcaattt ctttgcttgt gcagggtatg
      661 atgtagtaga taatttagga tttaaaacca tcgaagaagg tatagcagca gctaaaaaag
      721 ccaatgctga tatagtggta ttatgctcaa gtgatgatga atatgctgaa ttagcccctc
      781 aagcttttga tttagcaaaa ggaaacgcta ttttagtagt agcaggagct ccagcttgta
      841 ctgatgagtt aaaagcctat ggtattgagc attttatcaa tgttaaatcc aatgtgttgg
      901 atactttacg tacgtttaac ggcttattaa ctatcaatca atagagatga gtccaactac
      961 aaaaaattgg ataatagcta cacgcccatg gtcgtttccg gcatcggcaa tgcccgcttt
     1021 gtttgctatg gcgtatgttt tttacatgca agatagcata gagtccatcc attgggggta
     1081 tggagtagct gctttattgg gtgctgtctt gtttcaagca agcggaaatc ttattggcga
     1141 ttatttcgat tacatgtata aagtagatag aaaggaatcg ctcggttcga gccgtatgtt
     1201 ggtagatggt gtatttacgc ccaaaacaat attctggttt ggaataatcg tattatgtat
     1261 gggtattttg ctcggtttgt ttttatggtc gcaaacaggc aacgatttgc tatggattgg
     1321 tatcgttggt gctttgggta cattttttta ttacaaacta aagtatatgg ctttgggcga
     1381 tgtcttgatt tttgttattt acggaccgct cattgcttta ggaacagcct ttgtaatgac
     1441 gaatcaatta tattgggagg ttgtagtgtt aagtattccg attgcttttt tggttgtgaa
     1501 tattttacat gcaaacaaca cacgtgatat gagagatgat aaattggcaa atatcaaaac
     1561 acaagccttg ttgcttggcg aaaaaggttc gcgcattcaa tatacctttt tagctattgg
     1621 agcgtatgtg gctgtggcaa tatttgtatt attaaatatg atatcgcctt tggcattgtt
     1681 ggtaatagta accttgccac ttgcaaaaaa gaatatcaat ttaattaaac aagctaagat
     1741 tgataaacct gagattatca aagatttaga tgctatgtca gctcaattgg tgatggcatt
     1801 tacattgatg tatgctcttg gaatttttat tggatattgg atatgaaaaa agtattatta
     1861 gcgtcattgc ttgctgctgt tctgtggttt attatgtttt cgccatggac atctatccac
     1921 attaattttt ggatagcaat ggcttgttca gctgcaattt tgtgggctat gagtgctttt
     1981 ttggataaaa attttagcaa acaattctct ttcaatgtta aagatcttgt aataggactt
     2041 atgtctgctg ctgttttgta tggggtgttt gtagtaggta attatttatc tacccaatgg
     2101 tttgattttg ctggcactca aatacaaggt atttatggaa tgaaagaagg acaaaatttg
     2161 gtattagtag gtgtattact tactgtgctg attggtcctg ctgaagaaat attttggcgt
     2221 ggatatgtgc aaaaacaact tggtgcaaga tatggcgatt ggtttgctgt attgattact
     2281 gtgttaattt ataccttagt acatatctgg tcgtttaatt ttatgttaat tatggcagcc
     2341 atggtgtgtg gattgttttg gggcatattg tacaaatacc acaaaaatat tgtaacactc
     2401 attgtgtcgc atgctgtttg ggatgtagcg gtatttatcc taattccaat tgcataaata
     2461 attgagaaat aataaaaatg aataataaaa ttgagtacat tcctgaatgg ctcataaatt
     2521 aaaaaaaatg aacatgaaac ctgattttaa aagtattgac attacaaaca tcgctgtttc
     2581 atcaggcgtt ggtacacaag cggaaagtac ttgggtaaca cctgagcaaa ttcctgtgaa
     2641 accgttttac acaaaacaag acctaaatgg tatgcaacat ttacaatatg ctgcgggttt
     2701 acctccttat ttacgtggac cttatagtgt aatgtatgct atgcaacctt ggactattcg
     2761 tcagtatgcc ggattttcta ctgcagaaga atcaaatgcg ttttaccgta gaaatttagc
     2821 tgctggtcaa aaaggattgt ctgttgcttt tgacttggct actcaccgtg gatacgatgc
     2881 cgaccacgag agggtagtag gtgatgtggg taaagctggt gtatctatct gttcaatcga
     2941 agatatgaaa gtgttgtttg atggtattcc attgaataaa atgtctgtat caatgacgat
     3001 gaatggtgct gtattgcctg tgttagcttt ttatatcaat
//
LOCUS       DAOPUR010000159         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_33844_length_3004_cov_3.364869, whole genome shotgun sequence.
ACCESSION   DAOPUR010000159 DAOPUR010000000
VERSION     DAOPUR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33844_length_3004_cov_3.364869"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKN90_03675"
     CDS             complement(<1..209)
                     /locus_tag="PKN90_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNZ85411.1"
                     /translation="MQKITVQNPVVELDGDEMTRIIWSFIKEQLILPYLNVDIKYYDL
                     GMENRDATNDEVTVEAANAIKKYNVG"
     gene            complement(249..1604)
                     /locus_tag="PKN90_03680"
     CDS             complement(249..1604)
                     /locus_tag="PKN90_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNZ85412.1"
                     /translation="MEKEYLVYKLSETIKTTTRIDRELFTSYNVKRGLRNEDHSGVLV
                     GLTNIGDVVGYERLPEGGLKAIPGKLFYRGIDVEDLAKGIQKDNRVGFEETAFLLLSG
                     YLPDKEELDTFSSILNDSMVLEKKMKMNILELEGQNIMNILARSVLEMYTFDENPDDI
                     SRDNLIRQSIDLIAKFPTIIAYAYNMMRHSTHCRSLHIRHPKDKVSLAENFLYMMQGP
                     RSYSDLDVKILDLALILHAEHGGGNNSTFTVRVTSSSETDTYSSIAAGIGSLKGPLHG
                     GANLQVVDMLEHLKEAISDWTDAAELDAYLQKMLNKEVYNKTGKIYGIGHAIYTISDP
                     RALLLKEMARKLAEEKGLQKEFAFLELIENRAIETFMNFKGERINKQVCVNVDFYSGF
                     VYDAIGLPREVYTPLFAMSRIAGWTAHRIEELNFASKRIIRPAYKNVGGKKEFIPLQK
                     R"
     gene            complement(1619..>3004)
                     /locus_tag="PKN90_03685"
     CDS             complement(1619..>3004)
                     /locus_tag="PKN90_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNZ85413.1"
                     /translation="KHLQADTEVIANPENYYDRVIEINLNELEPYVNGPFTPDAAYPL
                     SEFAKIVKEKNYPQEMEVGLIGSCTNSSYEDLARAASVVAKAKETNIPVKAKLIINPG
                     SELVRYTAERDGLLETFESVGGVIMANACGPCIGQWKRESTTPDRPNSIVTSFNRNFA
                     KRNDGNPNTHSFVASPEIVAALTIAGDLCFNPLKDKLSTKNGELVKLPEPFGDELPKK
                     GFEVKDAGYIAPSENPDQAVVEIDPQSKRLQVLEAFSAWDGKDIIKQPLLIKVKGKCT
                     TDHISMAGPWLRYRGHLDNISDNMLIGAVNAFTDKTNAVLDTETGDYIAVPALARKYK
                     TAGIGSIVVAEDNYGEGSSREHAAMEPRFLNVKAIVVKSFARIHETNLKKQGLLALTF
                     VNKDDYSKIRENDTISILGVTNMKPGVPLTMVLHHVDGTEESFDVAHTYNLAQINWYK
                     SGSALNALNKI"
BASE COUNT          825 a          606 c          524 g         1049 t
ORIGIN      
        1 cctacattgt atttttttat ggcgttagca gcttctacgg ttacttcatc attggtggca
       61 tcgcggtttt ccatacccaa atcgtaatat tttatatcta cgttcagata aggaagtata
      121 agttgttctt tgataaatga ccaaataatg cgtgtcattt catctccatc taattctact
      181 acggggtttt gtacagttat tttttgcatc ggagtaatac gtttatggat aattatctgt
      241 ttttattatt atcgtttttg aagaggaata aattcttttt tccctccaac atttttgtat
      301 gcagggcgaa tgatacgctt gctagcaaag ttcaactcct ctatacgatg agccgtccat
      361 cctgctatac gcgacatagc aaatagaggt gtgtatactt ctcttggtaa gccgatagca
      421 tcgtatacaa atccagaata gaagtcaaca tttacacata cttgtttatt gatacgttct
      481 cccttgaaat tcataaatgt ttcaatagct ctattttcaa tcaattctaa gaatgcaaac
      541 tctttttgta atcctttctc ttcggctaat ttgcgtgcca tttcttttag caataaagcc
      601 cttgggtcag agatggtata aatagcatga ccgataccat agattttccc tgttttgttg
      661 tacacttctt tatttagcat tttttgtaaa taagcatcta actcagcagc atctgtccaa
      721 tcggaaatag cttcttttag gtgctccaac atatctacta cttgcaagtt agcacctcca
      781 tgaagaggtc cttttagtga accaatacct gccgctatag aagagtaggt gtctgtttca
      841 gacgagctag taacacgcac tgtaaacgtg gagttattac ccccgccatg ttcggcgtgc
      901 aaaatgaggg ctaagtcaag aatttttaca tccaaatccg aatagctacg tggaccttgc
      961 atcatgtaca aaaagttttc ggctaaagaa accttgtctt tagggtggcg aatatgcaac
     1021 gatcgacaat gcgtactatg acgcatcata ttgtatgcat aggcaataat cgttggaaat
     1081 tttgctatta ggtcgataga ttggcgaatg agattatccc gagaaatgtc atctggattt
     1141 tcatcaaacg tatacatttc gagcacgctt cgagccaaaa tattcatgat attttgccct
     1201 tcgagttcca aaatgttcat tttcattttt ttctcaagca ccattgagtc attcaggata
     1261 ctagaaaaag tatctaattc ttctttgtcg ggtaaatagc cagaaagtaa caaaaatgct
     1321 gtttcttcaa aacctacacg gttgtctttt tgaattcctt ttgctaaatc ctcaacatct
     1381 attcctctat aaaacaactt tcctggaata gcttttaatc ctccttcggg cagtctttcg
     1441 tagcccacca catcgccaat gttcgttaat ccaaccaata cgcccgaatg gtcttcgtta
     1501 cgcaaaccac gttttacatt ataggaggta aataattctc tatcaattcg tgtggtagtt
     1561 ttaattgttt ctgacaattt atataccaaa tattcttttt ccattctagt ttgtattgtt
     1621 aaattttgtt tagcgcattt agagcacttc ccgatttgta ccagtttatt tgtgctaagt
     1681 tataggtatg tgctacatca aatgattctt ctgttccatc aacatgatgt aacaccattg
     1741 ttaaggggac tcctggtttc atgttcgtta cacccaaaat actgattgta tcattttcgc
     1801 gaattttgga ataatcatct ttattaacaa atgtaagagc aagtaatcct tgctttttca
     1861 agtttgtttc gtgtattcga gcaaaagatt taaccacaat tgcttttacg ttcaagaaac
     1921 gtggctccat agcagcatgt tcgcgacttg agccttctcc ataattatct tcagccacca
     1981 caattgagcc tattccagca gttttgtatt tgcgagctaa cgctggtaca gcaatgtaat
     2041 ctcctgtttc ggtatcgagt actgcattcg ttttgtccgt aaaggcatta accgctccga
     2101 ttaacatatt atccgaaata ttgtctaagt gacctctgta acgcaaccaa ggaccagcca
     2161 ttgagatgtg gtcggtggtg catttccctt tcactttgat gagtaaaggt tgttttataa
     2221 tgtcttttcc atcccaagct gaaaatgctt ctaatacttg taatcgtttt gattgggggt
     2281 ctatttctac aacagcttga tcaggatttt cagagggagc tatatagcct gcatctttta
     2341 cttcaaaccc tttcttagga agttcatcgc caaaaggttc gggaagtttt acaagttctc
     2401 cattcttagt agaaagtttg tctttcaacg gattaaaaca caaatcacca gctattgtaa
     2461 gtgccgctac tatttcgggt gatgcaacaa agctatgtgt attaggattt ccatcatttc
     2521 tttttgcgaa atttctgttg aatgaagtta caatggaatt gggtctatca ggagttgttg
     2581 attcacgttt ccactgacca atacatggac cacaagcgtt tgccataata acaccaccta
     2641 cactttcgaa tgtttccaat agtccatctc tttcggcggt ataacgcacc aactcagaac
     2701 ctggattaat gattaacttt gcttttaccg gaatgtttgt ttctttcgct tttgctacta
     2761 ccgatgctgc cctagctaaa tcctcgtatg acgaattggt acatgaaccg atgagtccaa
     2821 cttccatttc ttgtggataa tttttttctt ttacaatttt tgcaaattca gacaagggat
     2881 aggctgcatc tggagtaaac ggaccattaa catatggttc taattcgttt aagttaattt
     2941 caatgactct atcgtaataa ttttctggat tagcaataac ttctgtatca gcttgcaaat
     3001 gttt
//
LOCUS       DAOPUR010000160         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_33949_length_2998_cov_3.225280, whole genome shotgun sequence.
ACCESSION   DAOPUR010000160 DAOPUR010000000
VERSION     DAOPUR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33949_length_2998_cov_3.225280"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..696
                     /locus_tag="PKN90_03690"
     CDS             163..696
                     /locus_tag="PKN90_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ85414.1"
                     /translation="MSTGGIIDNNKEMLDVINEDDKVIGQLTKKEIHNGINILHREIA
                     VLVHDGNGNLLLQQRSFKKKYFPGKWTVTAVGHVLAGQTPEEAAHMELKEELGFDTSL
                     NFVEKRKYISGDHTSLGYLFIGKFPTGQMIVPDPDELEKAGFFDLNGIKNMVSDNSID
                     AHSIETINKFLNGYYKY"
     gene            complement(756..1133)
                     /locus_tag="PKN90_03695"
     CDS             complement(756..1133)
                     /locus_tag="PKN90_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85415.1"
                     /translation="MNYRGIVVEESLQDKSILDSCKILSTDVAEIDSRTIWHIHKVQI
                     LEEQIHGFTKAIADKTDPNGTWYSHFYHEDPDKTQMIVTFYKKIFLVSKNNCSKAIEY
                     GLKQGVPAEQLDFQPRDVSKETW"
     gene            1481..2269
                     /locus_tag="PKN90_03700"
     CDS             1481..2269
                     /locus_tag="PKN90_03700"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85416.1"
                     /translation="MKRNTVGMWHLANTTYSGETIRMRSADNHVATAMVLTTVSQIWS
                     SGSSCDIQLVFTAVEEIYQLAMAGLNLRGSIGSEDDCIIVVEAMEMVPHGSGYPSYTA
                     GPLIKVNDGDVAYGVGANASYNLAEARLLQGATHTRHQHAESGGYTDATSVSLLTSCP
                     NIATLAVPCRNKHNVSTSGEVVPETVSYRDVVEGIGILKSAVLLEPVDAEGVLSIQLR
                     STNVAQRRRERVRAYRHNRARLAMGFYYPENILHWIGMVGSRLF"
     gene            complement(2312..>2998)
                     /locus_tag="PKN90_03705"
     CDS             complement(2312..>2998)
                     /locus_tag="PKN90_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNZ85417.1"
                     /translation="NEFTQAIGEKFVGKVIWYGESTINNCGYNLSGFKPLGLHNRLNM
                     LATIHVAEEIGMSTAEIQEQIPNIIVPKGRLNLILGISGSKLIDDTYNASPVSTKYAI
                     DTLVKYPSKRRIAILGDMLGLGTIEIQEHEKIGKYVAENPIDVFIGVGPLMKKAVEIA
                     KKTDIESYHLENSAKFKQELFLHLDINSGDTILVKGSQGIRMEKITEVLMEEPEKAPQ
                     LLVRQDIRWK"
BASE COUNT          969 a          547 c          551 g          931 t
ORIGIN      
        1 ggacgccgga ctttgaatcc gttaattggg gttcgactcc ctgtccccca gccaaggata
       61 tcattgtcat tgcaacattt taacaataga aaattagtat cactaatccc catttttata
      121 tagaaaataa gaatagaact cacagtgtta taataataaa acatgtctac agggggaatt
      181 atagataata ataaagaaat gttagatgtt ataaatgagg atgataaggt aatagggcag
      241 ttaaccaaaa aagaaataca taatgggata aatattcttc acagagaaat tgcggtttta
      301 gtgcatgatg gaaacggcaa cttgcttcta caacagcgta gtttcaaaaa gaaatatttc
      361 ccaggaaaat ggacagtgac tgctgtagga cacgttttgg ctggtcaaac tcctgaggag
      421 gctgctcaca tggaactcaa agaggaatta ggtttcgaca cttcattaaa ttttgttgaa
      481 aaaaggaagt atatttctgg cgaccataca tccttagggt atttgtttat aggtaaattt
      541 ccgacaggac aaatgatagt tccagaccca gatgagttag aaaaagctgg attttttgat
      601 ttaaatggta taaaaaatat ggtaagtgat aactctatcg atgctcattc aatagaaacg
      661 ataaataaat ttctaaatgg ctattataaa tattaaattc tattatctat ttacaatgta
      721 gttccaactt cttcccaaac ctccaataac aatatttacc aagtttcttt agatacatca
      781 cgaggttgaa agtctaattg ttctgcaggg acaccttgct ttaatccgta ttctatagcc
      841 ttactacagt tgttcttact aactaaaaat atttttttat aaaatgtaac gatcatttga
      901 gttttatcag gatcttcgtg gtaaaagtgc gagtaccatg ttccatttgg atctgtttta
      961 tcagcaatag ccttcgtaaa tccatgtatt tgttcttcta gaatttgaac tttatgaata
     1021 tgccaaatag ttctagagtc tatctctgca acgtctgtac tcagtatttt gcaagaatcc
     1081 aaaatacttt tgtcttgcaa actttcttct actactattc ctctgtaatt cataaattaa
     1141 ttataaccta atacaaaaag ctcaaatagc atgcaaaaca gccagccatg ctattcaatt
     1201 taaaaagaag ggaatatcaa aaaaatttat ttaataaggt ttaatactat ttctaggcaa
     1261 attttatatt ttttaacaaa caaaatacca aaaaagaaga cgagataaaa tataatcgat
     1321 atttacacaa ataataaatt aaccggaggt atttttaata tctccggtat tgtttggcat
     1381 caaattactt cttaaatttt gtaaaaagcg ttgtaaaacc taaaacacag aaaaagttga
     1441 tgtgcatgtt gaaaacgggg cattgcgaac aaggaagtct ttgaaacgta ataccgtagg
     1501 tatgtggcat ctggctaata caacctattc tggtgaaacg atccgaatga gatccgcaga
     1561 caaccatgta gcaaccgcaa tggtactcac aacagtgtcc caaatatggt catctggtag
     1621 ctcctgcgat atccagcttg tgtttacagc tgttgaggag atttatcaac ttgcaatggc
     1681 ggggttaaac ctacgaggca gtatcggatc agaagatgat tgcatcattg ttgtagaagc
     1741 tatggaaatg gtgccccatg gttcgggata cccgagctat acagcaggac cactaatcaa
     1801 agtcaatgat ggtgatgttg cctatggagt aggtgccaat gcaagctata acctagctga
     1861 agcgaggcta cttcaaggtg ctactcatac acgtcaccaa catgccgaat caggaggtta
     1921 cacagacgca acatcagtgt cgctcttaac gagttgccca aatatagcga ctctggcagt
     1981 gccttgtcgt aacaaacaca acgtctctac ctctggagaa gttgttccag aaacagtatc
     2041 gtacagagat gtcgttgaag gcatagggat tctaaaaagt gcagtattgt tggaaccagt
     2101 ggatgccgag ggcgtactga gcatacagct tagaagcact aatgtcgcac aacgtagaag
     2161 ggagcgtgtt agagcgtaca gacataatag agcaagactc gccatgggat tttactatcc
     2221 tgaaaatatc ctacattgga ttggcatggt gggttcaaga ttgttctgac catttgtggc
     2281 caggttgttt atcgcaagat agatgacctg gtcatttcca tcttatatct tgtctaacga
     2341 gcaattgtgg ggctttttca ggctcttcca ttagtacttc cgttattttt tccatacgaa
     2401 taccttgcga ccccttaacc aaaatagtat ctcctgagtt gatatctaaa tgtagaaata
     2461 gttcttgctt aaacttagca gaattttcca aatgataact ttcaatatct gttttcttag
     2521 caatttccac agcttttttc atgagaggac ctactccaat aaaaacatcg attggattct
     2581 ctgctacata ctttccaatt ttttcgtgtt cctgaatttc tatagtacca agccccaaca
     2641 tatcgcctag aattgctatt cttcttttag atggatactt tacaagtgta tcaatagcat
     2701 atttagtaga tacgggactg gcattatagg tatcatcaat taatttactg ccactaatac
     2761 caaggataag gttcaatcta cctttgggaa caataatatt gggtatctgt tcttgtattt
     2821 cagcggtact catgccaatt tcttctgcga catggatagt agctaacata ttgagtctat
     2881 tatgtaatcc caagggttta aaacctgaaa ggttataacc acaattattt attgtacttt
     2941 caccgtacca aattaccttt ccaacaaatt tctctccgat tgcttgtgtg aattcgtt
//
LOCUS       DAOPUR010000161         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_34127_length_2988_cov_3.551313, whole genome shotgun sequence.
ACCESSION   DAOPUR010000161 DAOPUR010000000
VERSION     DAOPUR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34127_length_2988_cov_3.551313"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PKN90_03710"
     CDS             complement(<1..297)
                     /locus_tag="PKN90_03710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNZ85418.1"
                     /translation="MKEYDYELTFKVRDYECDLQGIVNNANYQHYLEHTRHEFLLSRG
                     ISFSELHEKGIDAVIARIEIAFKYPLRSKDEFVSKLNIRKEGIKNVFYQDIYRLP"
     gene            complement(310..1572)
                     /locus_tag="PKN90_03715"
     CDS             complement(310..1572)
                     /locus_tag="PKN90_03715"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNZ85419.1"
                     /translation="MKKGNYVKLIVSILLFPMCSLMANVYHTQIFDTDIHTLRVYNPN
                     QKPYYPVVDLHVNEYVELSFDDLHPSFRLFSYKIIHCNADWTVSNATEIEYAEGFSTG
                     NIEDSSPSINTYVPYTHHSIRFPNENVRFKQSGNYAIVIYTNNDEQQVALTARVYVSE
                     NSITINGTVSGITDIDYKKEHQQLSIDIIPNNFTIHNPYRDIKVIVQQNQRMDNEVSN
                     VVPSIVQGNKISYINERKLIFAAGNEFRNFDLSATRILSRRIEDISFVQTQYHALLYP
                     DEIRKKAWYTQDYDINGRIIVNIQGTTENDTEADYFFVHFSLPSTLLPEDVYLLGQFN
                     HYHMDSSSIMKYNYEKRCYEKSLLLKQGGYDYAYVTRARESEKGTMNTIEGDYWETEN
                     EYIVYVYYRSFNDQSDRLIGKKIIRTTN"
     gene            1739..>2988
                     /locus_tag="PKN90_03720"
     CDS             1739..>2988
                     /locus_tag="PKN90_03720"
                     /inference="COORDINATES: protein motif:HMM:NF003761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNZ85420.1"
                     /translation="MAKVIHLKKGLDIKLKGAPLEIIGKATQSELIGFVPEHFAGIIP
                     KPVAKPGDKLKVGSVLFVDKNCPELRIVSPVSGELVAINRGERRKILDIVIKNDFKME
                     QEIFPTIDWKNASDKEVKQMLLSLGLFAFIKQRPYDIVANPNDQPKAIFISTFDTAPL
                     APDYTFILKDQVADFQAGITALSTLAPVSVGIKAGNGMFDGLKNATLHYFKGAHPAGN
                     VGVQINNVNPVNKGEVVWTINPQEVLFIGRYVNKGTLDFSKLVASTGSEVTTPAYYPS
                     IVGSPITPIVKANVSQGMSLRYISGNVLSGEKITENGFLNPFHSQISVIPEGDDNHEL
                     FGWIMPRWNKFSNSGTYFTKLVQFLNPKKTFEADARLLGGERHMIMSGEYDSVLPMDI
                     YPEFLIKAIIANDIDKMEALGIYE"
BASE COUNT          918 a          492 c          548 g         1030 t
ORIGIN      
        1 aggcaagcga taaatatctt ggtaaaatac atttttaatt ccttcttttc gtatgttaag
       61 tttgcttaca aattcatctt tgcttctgag tgggtactta aaggcaattt caatgcgggc
      121 aataaccgca tcaattcctt tttcatgcaa ttctgagaaa cttattcctc tagatagcaa
      181 aaattcatgt cgtgtatgtt ctaaataatg ttggtagtta gcattattta ctatcccttg
      241 caaatcacat tcatagtctc gcactttgaa agttagttca taatcatatt ctttcatatc
      301 aatgtaatgt taattggttg tccgaattat tttttttcca attaggcggt cgctttggtc
      361 gttaaaactt ctgtaatata catacacaat atattcattt tctgtttccc aatagtctcc
      421 ttctatggtg ttcattgtcc ctttttcgct ttcgcgagca cgtgttacat aggcataatc
      481 atatcctcct tgttttaaaa gtaatgattt ttcatagcat cttttttcgt aattatactt
      541 cattatagaa gagctgtcca tgtgataatg attgaactgt cccagtaggt atacgtcttc
      601 gggaagtagt gtagagggca acgaaaaatg aacaaaaaaa taatctgctt cggtgtcatt
      661 ttcagttgta ccttgtatat tcacaattat tctgccgttt atgtcgtaat cttgtgtgta
      721 ccacgctttt ttgcgtattt cgtcggggta gagtaatgca tgatactgcg tttgtacaaa
      781 agaaatatcc tctattcgac gacttaaaat ccgtgtggca gacaaatcaa aattacgaaa
      841 ttcgttgcct gctgcaaaaa tgagtttacg ttcgttgata tagctaattt tgttgccttg
      901 aacgatactg ggtactacat tacttacttc gttgtccatt cgttggtttt gttgtacaat
      961 tacctttata tcgcggtaag gattatggat agtaaaattg ttcggtataa tatctatact
     1021 gagttgttga tgctcttttt tataatcaat atcagtgatt ccagacactg taccattgat
     1081 tgtaatacta ttttccgata catacacacg tgctgttagt gctacttgtt gctcatcgtt
     1141 gtttgtataa attacgatag cataatttcc cgattgttta aatcgtacat tctcgtttgg
     1201 gaaacgtatt gagtggtgtg tataaggcac ataagtgttg attgatggac tcgaatcttc
     1261 tatgtttcca gtagaaaaac cttcggcgta ttctatttct gtggcattcg atactgtcca
     1321 atcagcattg caatgtataa ttttatacga aaaaagacga aacgatgggt gcaaatcgtc
     1381 aaacgaaagc tcaacgtatt catttacatg taaatcaaca accgggtagt aaggtttttg
     1441 gttaggattg taaacgcgta atgtgtgaat atcagtatca aaaatctggg tgtgataaac
     1501 attcgccata agagagcaca tcggaaaaag caatatgctc actatcaatt ttacgtagtt
     1561 tccctttttc attgttggca aagatataat taaaaaatga aactgcaaac tgtttttttg
     1621 atactattta ttatctgtaa aaaataaagt gttaaaaagg gaacttttat cttgtacata
     1681 tcgttatttt ttctaatttt gcgaagtttt tacaaaaaca gtattcaacc tcatttttat
     1741 ggctaaagta atacatctaa aaaagggctt agatattaaa ctcaaaggtg ctcctcttga
     1801 gataatagga aaagcgacac aatcggagtt gattggtttt gttcctgaac attttgcagg
     1861 aattattccc aaacccgtag ctaaacctgg tgacaaactg aaagtaggtt cagtattatt
     1921 tgtggataaa aattgtccag agttacgaat agtttctcca gtaagtggcg aattagtagc
     1981 tataaacaga ggagaacgtc gtaaaatttt ggatattgtt atcaaaaacg atttcaagat
     2041 ggagcaagaa atttttccta ctattgattg gaaaaatgct tcagacaaag aagtaaagca
     2101 aatgctttta tcactcggct tatttgcttt tatcaagcaa cgtccatatg atatcgtggc
     2161 taatccaaat gatcaaccca aagccatttt tatctctact ttcgataccg ctccattggc
     2221 tcccgattac acttttattt taaaagatca agtagccgat tttcaagcag gtattacagc
     2281 cttgtctacc ttagcacctg tctctgtagg gattaaagca ggcaatggga tgtttgatgg
     2341 tttgaaaaat gcaaccttac attattttaa gggagctcat cctgctggta atgtaggtgt
     2401 ccaaatcaat aatgtgaatc cagtaaacaa aggagaggtt gtttggacta tcaatcctca
     2461 agaggtattg tttattggtc gatatgtaaa taaaggtacc ttagatttct caaaattggt
     2521 agcatctact ggttctgaag ttacaacgcc agcgtattac ccatcaattg ttggttctcc
     2581 aataacaccg attgtaaaag caaacgtaag ccaaggtatg tctttacgct atattagtgg
     2641 taatgtgctt tctggtgaaa aaattactga aaatggcttt ttaaatcctt ttcattctca
     2701 aatctcagtt atacctgaag gagatgataa tcacgaattg ttcggttgga taatgccacg
     2761 ttggaataaa tttagcaata gtggtacata ttttactaaa ctagtgcaat ttttaaaccc
     2821 gaaaaaaaca ttcgaagcag atgcacgatt gttaggaggc gaacgacata tgataatgag
     2881 tggagagtat gatagtgtat tgccaatgga tatatatcct gaattcttga ttaaagccat
     2941 cattgcaaat gatatagata agatggaagc cttgggaatt tacgaaat
//
LOCUS       DAOPUR010000162         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_34275_length_2979_cov_3.261628, whole genome shotgun sequence.
ACCESSION   DAOPUR010000162 DAOPUR010000000
VERSION     DAOPUR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34275_length_2979_cov_3.261628"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..523)
                     /locus_tag="PKN90_03725"
     CDS             complement(101..523)
                     /locus_tag="PKN90_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85421.1"
                     /translation="MKRILLIFLITLMSGAYIYALVTPETLAKREKRKHLTVKEWNTD
                     AKGKTSWLDNLTIYDDQGRKVEEIEYANYGQRERITYQYDNATGKVEREIVYDYRNKP
                     TRIRKYEYNNDGTKKKQYNYLPNGKLFSIKVFEYIFTD"
     gene            complement(641..2137)
                     /locus_tag="PKN90_03730"
     CDS             complement(641..2137)
                     /locus_tag="PKN90_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNZ85422.1"
                     /translation="MANIKSLAKETAIYGVSSILGKFLNWLLVPLYTYVLESSADYGV
                     VTNLYSWTALLLVVLTYGMETGYFRFANKNKENADQVYSTTLVSVGSTSLLFALFCVF
                     FSQQIANFLGYPTHPEYVWMLLGIVVALDAFSAIPFAYLRYINRPIKFAALKFLYIVL
                     NIFFNLFFLIVCPWLLKEAPWTVSWFYNPHYGVGYVFVANLLSTIIQTLVLLPYVFAV
                     KFDFDMQLLKKILRYSIPLLLLGIAGIMNQTLDKILFPFLRTGVEGAAELGIYGATSK
                     IALVMLMFTQAFRLAYEPFIFAQQKDKNSLTTYALAMKFFIIFSLLIFLGMVLYIDIF
                     KYIIQQNYWEGLNVVPIVLFSFIFQGIFFNLSLWYKLTDKTIYGAWFSFLGTVIIVVG
                     NIVLVPLFSYTGSAWAAFLCYFVIMMVSYYFGQKYMPIKYDLKSIAIYSVLAICLYVI
                     SLFINTEYSWLDYLLKTVLLAIFVAFAIKRDMPLTSIPILGNWYAKKK"
     gene            complement(2166..>2979)
                     /gene="ruvB"
                     /locus_tag="PKN90_03735"
     CDS             complement(2166..>2979)
                     /gene="ruvB"
                     /locus_tag="PKN90_03735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNZ85423.1"
                     /translation="LSTIIANELQVGFKITSGPVLDKPGDLAGLLTSLETNDVLFIDE
                     IHRLSPIVEEYLYSAMEDYRIDIMIDKGPSARSIQLDLNKFTLVGATTRSGLLTSPLR
                     ARFGINCHLEYYDNDVLINIIKRSAAILHTPCTQDAAVEIASRSRGTPRIANALLRRV
                     RDFAQVKGNGKMDREIACYALEALNIDKYGLDEIDNKILTTIIDKFNGGPVGLTTIST
                     ALGEDAGTLEEVYEPFLIKEGFLKRTPRGREVTQLAYEHLGRIRKADIGSLF"
BASE COUNT         1041 a          560 c          439 g          939 t
ORIGIN      
        1 attaatttta cggctttcat ttccgagagc gaaatggtat ccattgctct caaaactgcc
       61 aattttgtta attcgtctct agtcattttt atagatcaaa ttaatcagta aatatatatt
      121 caaatacttt aattgaaaat aacttaccat ttggcagata attgtattgc tttttctttg
      181 tgccgtcgtt attgtattcg tatttacgta ttcttgtagg tttgttacga taatcataaa
      241 caatctcccg ttctacttta ccagtggcat tatcgtattg ataagttata cgttcccttt
      301 gtccataatt agcatactca atctcctcta cttttctccc ttgatcatca tatattgtta
      361 aattatccaa ccaactggtt ttacctttgg catctgtatt ccactctttt acagttaaat
      421 gttttcgctt ttcgcgtttg gctagtgttt ctggtgttac taaagcatat atatatgctc
      481 cagacataag ggtgataaga aaaattaata gaattctttt cataatacat gtataaggtt
      541 gtaacaaaaa aatatattaa ctaaaatggc taacaaatat agctacactt ctgttaagag
      601 aacgcaaaga tataattaaa catagaaact tcaaattatt ttactttttt tttgcatacc
      661 aattgccaag aatgggtata gatgttaacg gcatatctct tttgatggca aatgctacaa
      721 aaattgccaa taagacagtt ttcagcaaat agtccaacca actatattcc gtattgataa
      781 ataatgaaat gacatataag caaatagcta atacagagta gatggctatt gattttaaat
      841 catactttat aggcatatat ttttgtccaa aatagtatga taccatcata ataacaaaat
      901 agcacaagaa agcagcccat gccgatcctg tatagctaaa aagaggtact aaaacgatat
      961 tccctactac aatgattact gttcctaaaa aagagaacca tgctccgtaa atggttttat
     1021 ccgtaagttt ataccacaaa gaaaggttga aaaatattcc ctgaaaaatg aaggagaaca
     1081 aaacaatagg aacaacgttt aacccttccc aataattttg ttgaatgatg tatttaaaaa
     1141 tgtcaatata taaaaccatt ccaaggaaaa ttagcaagga aaaaatgatg aaaaacttca
     1201 tggctaaagc atatgttgtc aaactattct tatctttctg ttgagcaaaa ataaatggtt
     1261 catacgcaag ccgaaaagcc tgagtaaaca taagcatcac caatgctatc ttagatgttg
     1321 ctccataaat tccaagttca gcagctcctt ctactcctgt cctcagaaat ggaaaaagaa
     1381 ttttgtccaa tgtttgattc ataatgcctg cgatacctaa aagcaacaat ggaattgaat
     1441 agcgtaagat ttttttcagc aattgcatgt caaaatcaaa ttttaccgca aaaacatatg
     1501 gtaataaaac taacgtttga ataattgtag acaataaatt tgcaacaaat acataaccaa
     1561 caccataatg gggattataa aaccagctta ctgtccacgg ggcttctttc agtaaccaag
     1621 gacatacaat cagaaaaaaa agattgaaga aaatattcaa tacaatatac aaaaacttga
     1681 gtgctgcaaa ttttattgga cggttgatgt atcgtaaata agcaaacggt attgcagaaa
     1741 aggcatctaa tgcaacaacg attccgagca acatccaaac atattcagga tgtgtcggat
     1801 agccaagaaa attggctatt tgctgcgaaa agaatacaca aaataatgca aataataatg
     1861 atgtactccc aactgaaact aacgtagtac tgtacacttg atcggcattt tctttgtttt
     1921 tgttggcaaa acgaaaatat ccagtttcca taccgtatgt caaaacaact aacaacaaag
     1981 ctgtccaaga atacaagttt gttactacac cataatcggc agaactttcc agaacatagg
     2041 tataaagagg aacaagtaac caattaagaa attttcctaa aatgctactt actccatata
     2101 ttgctgtttc ctttgcaaga ctttttatat ttgccatgaa tacgattatt ctgatgaata
     2161 aaaacctaaa ataaagaacc tatatctgct tttctaatac gacctagatg ctcatatgcc
     2221 aattgcgtta cctctctacc acgaggtgtg cgtttcaaaa aaccttcctt tattagaaac
     2281 ggctcgtaca cctcctctag agttccagca tcttcgccca aagctgtcga aatggtagtc
     2341 aatccgacag gtcctccatt aaatttatca ataatagtag tcaaaatttt gttatcaatc
     2401 tcatctaaac catacttatc aatatttaat gcttcgaggg cataacaagc aatttctctg
     2461 tccatttttc cattcccttt tacttgagca aaatctctta ctctacgcaa taaggcatta
     2521 gcaatacgtg gggtacctct acttctgcta gctatctcta cagctgcatc ttgagtgcat
     2581 ggggtgtgta atatagctgc cgaacgcttt atgatattga taagcacatc attgtcgtaa
     2641 tattctaaat gacaattgat gccaaatcgg gctcttaacg gactagtaag cagtccacta
     2701 cgagtagttg ctcctacaag tgtaaatttg tttaaatcta gttgtataga gcgagcactt
     2761 ggacctttat ctatcattat atcaatacga taatcctcca ttgctgaata taaatattct
     2821 tctacaatcg gactcaaacg atgtatttca tctataaaca acacatcatt cgtttccaaa
     2881 ctggttagca aacctgctaa atcaccaggt ttatccaata caggtcctga agttatttta
     2941 aacccaactt gcaactcatt ggcaattata gttgataaa
//
LOCUS       DAOPUR010000163         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_34409_length_2971_cov_3.145405, whole genome shotgun sequence.
ACCESSION   DAOPUR010000163 DAOPUR010000000
VERSION     DAOPUR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34409_length_2971_cov_3.145405"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..180
                     /locus_tag="PKN90_03740"
     tRNA            105..180
                     /locus_tag="PKN90_03740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:141..143,aa:Pro,seq:ggg)
     gene            complement(290..1513)
                     /locus_tag="PKN90_03745"
     CDS             complement(290..1513)
                     /locus_tag="PKN90_03745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ85424.1"
                     /translation="MKVRNWTKWTPLFLSNFMGVLNDNFLKYSIIFMAISWSLPTWFT
                     QSQLIALVSFALVIPYVFLSPLGGMLATRYSKRKVFLFFKLLEIPIMAIACVAFYYQY
                     VFLAVFSVLLMGIQSSLYSPAKYSLIRDIGGEKGVSFGTGVFEMMAFLGILAGTVLAS
                     YMADRYSYVWVLICFLGFAIIGYLVTSQIRAKEIAAPQELKIALHPFAFIIQSHHKSR
                     AFPMLNAAVFGSAFFWFLGSILQMNIVIHSTVVYRLSNTETGVIVSIVAIALGVGCWI
                     AGKILTNTNIYVLLLVGISGIAISSFVLGIYCYPLRTFLALLVILSLCGGLFQVTCLS
                     LIQKTAKEISLADIFAYVNLLTFICILIGAGLFSLINWMSGDNSFYIFFLLSAISFLL
                     TFFYFFKVMSIRKLH"
     gene            complement(1720..2655)
                     /locus_tag="PKN90_03750"
     CDS             complement(1720..2655)
                     /locus_tag="PKN90_03750"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ85425.1"
                     /translation="MKNHYTKKHVVAKVVLLSVLFFCSSYSVFSSSLKRYQVSGLGGD
                     LTNANGVYIEEGTYGELDWPYFKHETNNYFLYFEYYSSFVPPLGMPTIGTWAIGSNLG
                     QVLCYLNEIYNPMEFEEPNGLGYILLKNITVVLTATTQEAINISSTSATLNATVRAEN
                     ATGEVSFEYGTTASYGTEVSATPNQVSGSEDVPVSVTIADLASNTSYYYRVKIESGDE
                     IVYGDNFTFQTEDISSNVETDAIQQIVLYPNPAKDGFYIDAKEQGYVCMYDLSGQLVL
                     KQFIDAVSYIDIQSLAKGVYVVKLHERSRILLKTE"
BASE COUNT         1081 a          554 c          460 g          876 t
ORIGIN      
        1 aaagcagcta ttttagctgc ttttttcgta taataatagc gaataaatta gatttttgat
       61 ttgacaaaca caaaaaatta cgtacatttg cattcccaat agttcggggt gtagctcagc
      121 ccggtttaga gtacacgtct ggggggcgtg tggtcgcaag ttcgaatctt gtcaccccga
      181 ctaacaacaa agcaagactt tccacaagaa agtcttgctt tgttgtatag ggacatgatg
      241 tttccacaac aaacaattat ttcaggtctt tgctttttat tctatttatt caatgcaact
      301 ttcgtataga cattacctta aaaaaataaa aaaacgtcaa caaaaaagat atcgcagata
      361 aaagaaaaaa tatataaaaa ctattatcac ctgacatcca atttattaga gaaaataatc
      421 cagcaccaat taagatacat ataaaagtaa gcaaattaac ataagcaaaa atatccgcta
      481 acgaaatttc cttagctgtt ttttgaataa gagacaaaca cgtaacttga aataatccac
      541 cacacaggga gagaatgacg agtaatgcta aaaatgtccg taatggatag caatatatac
      601 ctaaaacaaa gctactaatt gctattccac taatacctac caataatagt acatatatat
      661 tggtgttcgt taaaatttta ccagctatcc aacaccccac tcccaatgct atggctacta
      721 tggacacgat gacacccgtt tctgtattag ataatctgta tacaacagta ctatggatga
      781 cgatattcat ctgcaatata ctacctaaaa accagaaaaa agccgatcca aacacggcag
      841 catttaacat gggaaaagct cgagatttat gatgcgattg aataataaat gcaaatggat
      901 gtaatgctat tttaagctct tgaggagcag caatttcttt tgctcttatt tgagatgtaa
      961 ctagatagcc aataatagca aaacctaaga aacatatcaa tacccagaca taactatacc
     1021 tatcagccat gtaagatgcc aatactgtac cagctaatat tccaagaaat gccatcattt
     1081 cgaatacacc cgttccaaac gacacccctt tttctccccc aatatcacgt attaaactat
     1141 atttggcagg agaatacaag ctactttgaa tccccattaa caaaacggaa aaaacagcta
     1201 aaaacacata ttgataataa aacgccacac aagctattgc cataatgggg atttctagca
     1261 acttaaaaaa aagaaaaacc ttgcgtttgg aatagcgagt tgccaacata ccgcctagag
     1321 gagataaaaa aacatagggt attactaacg cgaatgatac cagggctatt aactgagatt
     1381 gagtgaacca agtaggcaat gaccatgata tcgccataaa aataatggag tatttcagaa
     1441 aattatcatt taatacaccc ataaaattgc taagaaacaa gggtgtccat ttagtccaat
     1501 tacgtacttt catctgtctt ggttatctta cattttaatt ttgaacaaga caaaagtacc
     1561 tatatttttt tatatgcaat aaaaaaatta gtttctgata caagattgtt tgaagtatag
     1621 tatcataagg attacacaac aaaagccctg cgtatctatc aggtagttcg cagggcttag
     1681 taaagaaaaa aattatcgct taacaaaaaa catgactcat cattctgttt ttaacaatat
     1741 tctactcctt tcatgtagct ttactacata cacccctttg gcaagtgatt gaatgtcgat
     1801 gtatgaaact gcgtctataa actgttttaa aactaactgt ccacttaaat cgtacataca
     1861 tacatatccc tgttctttag catctatata aaagccatct ttagcaggat taggatacaa
     1921 tacaatctgc tgtatggcat cagtctccac attgctagaa atatcttccg tttgaaaagt
     1981 aaaattatcg ccatatacta tttcgtctcc actttcaatt tttactctgt aataataact
     2041 agtattggag gctaaatcgg caatagttac gcttactggc acatcttcag aaccagacac
     2101 ttgatttggt gtagctgaaa cttctgtgcc ataactagca gtcgttccat actcaaaaga
     2161 aacttctcct gtggcattct cagcacgcac tgtggcattt agggtagcac tggtagagga
     2221 aatatttatt gcttcttgcg tagtagcagt taacacgact gtaatatttt ttaaaaggat
     2281 atatcctaat ccatttggtt cttcaaactc cataggatta taaatttcgt tcagataaca
     2341 cagtacttgt cctaaattgc taccgatagc ccaagtccca attgttggca tacccaaagg
     2401 cggtacaaag cttgaataat actcaaaata taaaaaatag ttattcgttt cgtgcttaaa
     2461 gtaaggccaa tctaactctc cgtatgtccc ttcctctatg taaacaccat tagcattagt
     2521 tagatcacct ccaagacccg agacttgata tcgtttgagt gaagaagaaa atacgctata
     2581 cgaagagcaa aagaatagca cagatagtaa aacgactttc gctactacat gtttttttgt
     2641 gtaatgattt ttcataagca gtaattttaa ggtaaaacat aacgtaacac tattttaata
     2701 atgctaaggt atgagtattg cattacactc actactaaaa aaaacaaaat agctgcctaa
     2761 aattacaatt tcagaaatgt aaaacagaga aaatgagaag gattgatgaa taaaaactaa
     2821 atacctttgt ttttgatgtt ctctttgtat tgagaaggcg aaactgtagt aagtttatta
     2881 aaccataaat taaatgacga gatggaagca aaaccagttt taatggcaat ttcagataca
     2941 ggtaaattag gattattttc taatagcatt a
//
LOCUS       DAOPUR010000164         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_34450_length_2969_cov_3.843514, whole genome shotgun sequence.
ACCESSION   DAOPUR010000164 DAOPUR010000000
VERSION     DAOPUR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34450_length_2969_cov_3.843514"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PKN90_03755"
     CDS             <1..1105
                     /locus_tag="PKN90_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNZ85426.1"
                     /translation="ACETLNSDVVKLLLANGAEVDSDCFVSLFQRDIIDIEELQSIID
                     LSKILIDKNININTRNNEFEGNTYSSTALMYAASYKHDEDLGTSAKLLLEKQVELMGF
                     LLDNGAEINAVNTQRKTPLMCAIEAKNEHIVAYLLERGADTNIKDNKGNTALTYAYIN
                     SVKVIGELRKKMNVEQTYQIDKDAIPNQESIVNLYQFIYNIIPYLANEVFTGNLGFEQ
                     VSDKNWIKRIVAQSYQISFNWDGFEVDNIQKTFFKNKPSVLMILFTFPETNEPALAKY
                     ALLTIDLENKSFANYNTLEITGIDNYMMIGGVGIISQTQIDDEFSDNLYAHLNYGPVQ
                     YEANPKIFLRDMFTRLSNDNEQLSFLKHIKDEI"
     gene            1316..2380
                     /locus_tag="PKN90_03760"
     CDS             1316..2380
                     /locus_tag="PKN90_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85427.1"
                     /translation="MKKAAKILCIIAMIIGLIWAVVGFFGTWIGGAVVGAVQETVENS
                     ESANATMEKSVSIMLRLLGSFVVVIIGGVLGIVGANKKPSLNPIILGILTFMCGYLLF
                     PLSNYLAAALYLVAGLLLFLAGVTTKQVENIDDEQLRKKKLSLTITGIAMALVIAVIS
                     HFIFKDSSELQPNTSNTVAIEKQQEQSETSENLGIWEVAYFVDDFGEPTKDGYIRTEI
                     KGTFSNDLTQNSKLGVRFIITDAEIIDMKFYQYNGNSPTVLYVETSYRLLVKDSKEKV
                     HTLMAYHNHDRLSLTPMESKKLYQILLQGGAIKFNIVSSNDFSNRDTEYNFTIDNADG
                     FENAVMKLIDSNEIDTRPAI"
     gene            2646..>2969
                     /locus_tag="PKN90_03765"
     CDS             2646..>2969
                     /locus_tag="PKN90_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85428.1"
                     /translation="MKTPSLISGNSWGAEIFSDSRVIASDTIEFPCLTRCSKCDIIFW
                     LSKLEEIGTLGWDEDEIVNPDWEKADWARKLTVEEYFESLNEGLAENKEDEIFIRQQI
                     WWGYND"
BASE COUNT         1056 a          430 c          572 g          911 t
ORIGIN      
        1 agcttgtgaa acattaaata gtgatgtagt aaaattactg cttgcaaatg gtgcggaggt
       61 tgattctgat tgttttgtaa gcttatttca acgtgatatt attgacatag aagaactaca
      121 aagtataata gatttgtcaa aaattttaat tgataaaaac ataaatatca atacaagaaa
      181 caatgaattt gaaggaaata cctatagctc tactgcctta atgtatgctg caagttataa
      241 acatgatgaa gatttaggaa cttctgcaaa attactttta gaaaaacaag tagagcttat
      301 gggttttctt ttggataatg gggctgaaat taatgccgta aacacacaaa gaaaaacccc
      361 actgatgtgt gctattgaag ctaaaaatga acatattgta gcatatcttt tagaacgtgg
      421 tgcggatacc aatatcaagg ataataaagg caatacggct ttaacctacg cttacattaa
      481 ttcagtaaaa gtaattggag agttacgcaa gaaaatgaac gttgaacaaa cataccaaat
      541 cgacaaagat gcaataccaa atcaggaaag catagtaaat ttgtatcaat ttatctacaa
      601 cattattccg tatttagcaa atgaggtttt tacaggaaat ttaggttttg aacaggtgag
      661 tgataaaaac tggattaaac gtattgttgc acaaagctat caaatttctt tcaattggga
      721 tggctttgag gtggataata tccaaaaaac atttttcaaa aataaacctt ctgttttgat
      781 gattttgttt actttcccag aaacgaacga gccagctctt gcaaaatatg ctttgctgac
      841 cattgatctt gaaaataaat ctttcgccaa ctataatacg ctcgaaatta caggcattga
      901 taattacatg atgattggag gcgttggaat tatttcgcaa acgcaaattg atgatgaatt
      961 ctctgataat ttgtatgctc acctcaacta tggtccagta caatacgaag ctaacccaaa
     1021 aatctttttg cgtgatatgt ttacgcgttt gagcaatgac aacgaacaac tttctttttt
     1081 gaaacacatt aaagacgaaa tttaaaaaca tagaagcatt agcttttatt cttacagtgg
     1141 gaaattgcta attcttgacg catacaggaa aaatacgtaa taacgtgaac taaacttttt
     1201 tgtatctgtt gatgactagc aatgcctcaa ctgcggatat agctatagcc ctatgcttcg
     1261 ttcttaacaa tcaccatact tggggcttgt gtggcaaact taacaaatat attttatgaa
     1321 aaaagcagca aaaattttat gcattatcgc tatgattatt ggattaattt gggcggtagt
     1381 aggttttttt gggacttgga ttggaggagc agttgtaggt gctgttcaag aaacggttga
     1441 aaattctgaa agtgcaaacg caactatgga gaaaagtgta agtataatgc ttaggctttt
     1501 gggtagtttt gtagttgtaa ttattggtgg tgtgttgggt attgtgggtg ctaataaaaa
     1561 gccatcatta aatccaatca tactaggaat tcttacgttt atgtgcggat acctattatt
     1621 cccgctaagt aattatcttg cagcggcact ttatcttgtg gcaggtttac tgctattttt
     1681 ggcaggtgtt acaacaaaac aagtagaaaa cattgatgac gagcaactga gaaagaaaaa
     1741 actaagtttg actataacag gcattgctat ggcattggtg atagcggtga taagtcattt
     1801 tattttcaaa gatagttcgg aattacagcc taacacatca aacactgtag ctattgaaaa
     1861 acaacaagag caatcggaaa caagcgaaaa tttgggcatt tgggaggttg catattttgt
     1921 agatgatttt ggcgaaccaa caaaagatgg atatatccga actgaaatta aaggaacttt
     1981 ttcaaatgat ctgactcaaa attctaaatt aggagtacgt tttataatta cagatgcaga
     2041 aattattgat atgaaatttt atcagtataa tggtaatagt cctactgttt tgtatgttga
     2101 aacttcttat agattgcttg tgaaagactc aaaagaaaag gtacatacat taatggctta
     2161 tcacaaccac gataggttat cgttaactcc tatggaatca aaaaaactgt accaaatctt
     2221 attgcaagga ggagctatca aatttaatat tgtaagctcg aatgatttca gtaatcgtga
     2281 cactgaatac aactttacca ttgacaatgc tgatgggttt gaaaatgcag taatgaaatt
     2341 gattgatagc aatgaaattg acacaaggcc tgctatctaa aaataccgtt caaactgtaa
     2401 ctgaagcttt tctttagaaa tctggtaggc gtaggttgta cctatgccat actagatagc
     2461 aacaaaaaaa atacatttga agaagtgatt ctttctgtaa aagaacaatg ttattacaca
     2521 atttttatcg cttggtaaaa atacaaacag gattattaca cgaataaata caaatttaac
     2581 aacaaaatac aactatgata ttaggaccag actatgttta caaatgccca aattgtggca
     2641 gctttttgaa aactccgagt ttaataagtg gaaactcctg gggtgcggaa atattttcgg
     2701 acagcagggt aattgcttct gacactatag aatttccttg cctaaccagg tgtagcaaat
     2761 gtgatataat cttttggtta agtaaattag aagaaattgg aacattggga tgggacgaag
     2821 atgaaattgt aaatccagat tgggaaaaag cggattgggc tagaaagtta accgtggaag
     2881 aatactttga atcccttaac gaaggacttg cagaaaataa ggaagatgaa atatttatac
     2941 gtcaacaaat ttggtggggg tataatgac
//
LOCUS       DAOPUR010000165         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_34541_length_2964_cov_2.516672, whole genome shotgun sequence.
ACCESSION   DAOPUR010000165 DAOPUR010000000
VERSION     DAOPUR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34541_length_2964_cov_2.516672"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2964)
                     /gene="carB"
                     /locus_tag="PKN90_03770"
     CDS             complement(<1..>2964)
                     /gene="carB"
                     /locus_tag="PKN90_03770"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HNZ85429.1"
                     /translation="KIGEAGEFDYSGSQALKALKEEGIYTVLVNPNIATIQTSEGVAD
                     QIYFLPITPFFVEKVIQKEKPDGILLAFGGQTALNCGVQLHRLGILEKYNLRVLGTPV
                     QAIMDTEDRDLFVKKLDQIDVKTIKSFAVDNMEDARKAAKDLGYPIIIRAAYALGGLG
                     SGFCDNEEELDAMAEKSLSFSPQILVEKSLKGWKEVEYEVVRDRFDNCITVCNMENFD
                     PLGIHTGESIVIAPSQTLTNAEYHKLRELAIRIIRHIGIVGECNVQYALDPESEDYRV
                     IEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLFDVKNSVTKTTPAFFEPALDYVV
                     CKIPRWDLGKFQGVSRELGSSMKSVGEIMAIGRTFEEAVQKGLRMIGQGMHGFVANKE
                     LVIADIDKSLNEPTDNRIFVISKAFRKGYTIDQIHDLTKIDKWFLQRLYKIVETANEL
                     EAFNSVEDVDDELFRLAKQQGFSDFQIARLTSDSAESEESLIKVRNNRKARGITPVVK
                     RIDTLAAEYPAQTNYLYLTYNGTANDMQYLGDKRSIIVLGSGAYRIGSSVEFDWCSVN
                     ALETIRKEGWRSVMINYNPETVSTDYDTCDRLYFDELSFERVMDIVELENPHGLIVST
                     GGQIPNNLALRLNNMNVPLLGTTATSIDRAEDRHKFSSMLDSLKIDQPRWKELSSLED
                     VQLFVDEVGFPVLVRPSYVLSGAAMNVCSNKEELKEFLQLAANVSKKHPVVVSEFIES
                     AKEIEMDAVADKGEIMMYAISEHIEFAGVHSGDATIQFPPQKLYLETMRRVKKIAHQI
                     AKELCISGPFNIQFLAKNNEIKVIECNLRASRSFPFVSKVLKINFIELATKIMLGIPV
                     QKPNKNAFNLDYVGIKASQFSFSRLQKADPVLGVDMASTGEVGCIGDDADEAILTSML
                     SVGYTIPKKNILLSTGTPKQKVDMLDAARLLLKKGFNLYATGGTHTFLQENDIPATLV
                     HWPSEEMQPQALEMLK"
BASE COUNT          873 a          644 c          519 g          928 t
ORIGIN      
        1 tttgagcatt tcaagagctt gtggttgcat ttcttcgctg ggccaatgca ccaaggtagc
       61 aggtatatca ttttcttgca agaaggtgtg tgtaccaccc gttgcatata gattaaagcc
      121 ttttttaagc aacaaacgag ctgcatcaag catatctact ttttgttttg gtgttcctgt
      181 tgataataaa atattctttt tcggaatagt ataacctaca gatagcatag aagttaatat
      241 tgcctcatca gcatcatcgc caatacatcc cacttcgcct gttgaagcca tatccacacc
      301 caatactggg tcagcttttt gcaaacgaga gaaagagaat tgagaagctt tgatacctac
      361 gtaatctaag ttaaaggcat ttttgttcgg tttttgaaca ggtatgccca acataatttt
      421 ggttgctagt tcaataaagt taatttttag cactttagat acaaatggaa aactacgaga
      481 agccctcaaa ttacattcga taaccttaat ctcgttattt ttagctaaaa attgaatatt
      541 aaatggacct gagatacata gttctttcgc aatttggtga gcgatttttt tcacccttct
      601 cattgtttct aagtacaatt tttgaggagg aaactgaata gtagcatcac cagagtgtac
      661 accagcaaac tcgatgtgtt cggaaatggc atacatcatt atttcccctt tatcggctac
      721 cgcatccatc tcgatttctt tggcactttc gataaattcg cttactacca cagggtgttt
      781 cttagacaca ttagcagcta attgcaaaaa ttctttcagt tcttctttgt tagaacacac
      841 attcattgcc gctccagaaa gcacatacga tggacgaaca agcacaggga aacctacttc
      901 gtcgacaaaa agttgtacat cttcgagcga agacaactct ttccaacgtg gctggtcaat
      961 ttttaaacta tccaacatcg acgagaactt atgtctatcc tcagctctat caatactggt
     1021 ggctgttgta cccaacaatg gcacgttcat gttatttaaa cgtaaagcta agttatttgg
     1081 aatttgacca cctgtagaaa cgattaatcc atgtggattt tctaattcta cgatatccat
     1141 tacacgttcg aacgataatt cgtcgaaata taaacggtca caagtatcat aatccgtaga
     1201 aaccgtttcg gggttataat ttatcattac cgaacgccaa ccttctttgc gtatagtttc
     1261 tagggcattt acactacacc agtcgaactc taccgagcta ccaatgcggt atgctccaga
     1321 gccaagtaca ataatagagc gtttatcgcc caaatactgc atatcgttgg ctgtgccatt
     1381 gtaggttaaa tacaagtaat ttgtttgagc tggatactca gcggctaagg tatcaatacg
     1441 tttcactaca ggagtaatac ctctagcttt gcggttgttt cttactttta tcaaactttc
     1501 ttcactctca gcactatcgc ttgtaagacg tgctatttgg aagtcagaaa agccttgttg
     1561 tttagccaaa cggaataatt catcatcaac atcttctacc gaattgaatg cttccaattc
     1621 gttggctgtt tcaacaattt tatataatcg ttgtaaaaac catttatcta ttttggttaa
     1681 atcatgaatt tggtcgatgg tatagccttt acgaaatgct tttgagataa caaaaatacg
     1741 gttatctgtt ggttcgttta gcgatttgtc aatatcagca attaccagtt ctttattggc
     1801 aacaaaaccg tgcattcctt gaccaatcat acgcaagcct ttttgcacag cttcttcaaa
     1861 cgtacgtccg atagccatga tttcgcctac tgatttcatg cttgagccta actcgcgcga
     1921 aacgccttga aacttgccca aatcccaacg aggaatttta cacaccacat agtcgagtgc
     1981 aggttcaaaa aacgcaggag tagttttagt tactgaattt ttgacatcga acaacccata
     2041 ccccaatccc aattttgcag ctacaaaagc caatggatag cctgtagctt tagaagccaa
     2101 agccgaagag cgacttaaac gggcatttac ctcaattaca cgataatctt ccgattctgg
     2161 gtcgagagca tattgcacat tacactcacc cactataccg atatggcgaa tgattcggat
     2221 tgccaattca cgcaatttgt ggtattcggc attcgtaagc gtttgcgaag gagcaataac
     2281 gatactttcg cccgtatgaa taccgagcgg gtcgaaattc tccatattac ataccgtaat
     2341 acagttgtca aaacggtcgc gaactacctc gtactcaacc tctttccacc cttttaatga
     2401 tttttcgact aatatttggg gagagaaaga caatgatttt tcagccattg catctaattc
     2461 ctcttcatta tcacaaaatc cactacctaa accacctaag gcataagccg cacgtataat
     2521 aattggatac cccaaatctt tagcagcttt gcgagcatct tccatattat ctacagcaaa
     2581 acttttgatt gtttttacat caatttggtc gagttttttc acgaacaaat cgcggtcttc
     2641 tgtatccata attgcctgta cgggagttcc caatacacgc aaattgtatt tttctaaaat
     2701 tcctaatcgg tgcaattgta caccacagtt aagagctgtt tgaccaccaa aagccaacaa
     2761 aataccatca ggtttttctt tttggatgac tttttcgaca aaaaatggcg taatgggtaa
     2821 gaaataaatc tggtcggcta ccccttcgga tgtttgaatg gtagcaatat ttggattgac
     2881 caaaacggtg taaattcctt cttcttttag tgctttcaga gcttgagagc ctgaataatc
     2941 aaattcgcca gcttccccaa tttt
//
LOCUS       DAOPUR010000166         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_34568_length_2962_cov_3.057792, whole genome shotgun sequence.
ACCESSION   DAOPUR010000166 DAOPUR010000000
VERSION     DAOPUR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34568_length_2962_cov_3.057792"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PKN90_03775"
     CDS             complement(<1..485)
                     /locus_tag="PKN90_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ85430.1"
                     /translation="MQLSDRTIGGWLEYWAKETPNKECIVYSDRNLRFTWHDFDQRVN
                     DLAKGLMAIGIEKGHHVGIWATNVPDWLTFLFATSKIGAVLVTVNTNYKQHELEYLCQ
                     NSDMHTLCIIDGTWECDYVDMTYTMLPELKTSQRGYLNSERFPKMKNVVYIGQEKFRG
                     M"
     gene            complement(535..1113)
                     /locus_tag="PKN90_03780"
     CDS             complement(535..1113)
                     /locus_tag="PKN90_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNZ85431.1"
                     /translation="MSQNNAFVGKKIKTIREGKDITVEQLAERSGLAVEQIRSIEEDE
                     KLPSLAPLTKIARALGVRLGTFLDDNTEIGPVVCRKGEKSGSLSFSNDMVGARKHMEY
                     YSLAGQKAGRHMEPFIIDIQPSVSKDFILSAHEGEEFIYVLSGEIEINYGKAVYHLQE
                     GDSIFYDSIVEHHVHAHKEKGAKILAVVYSPF"
     gene            1431..2468
                     /locus_tag="PKN90_03785"
     CDS             1431..2468
                     /locus_tag="PKN90_03785"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNZ85432.1"
                     /translation="MRLLTGKNRWLLHITYWVMVVLLFTFFWGTRYGNYWVCFYNELY
                     FLPIKIAATYFGLYYLIPQLFKRNYYRVVVAGSFMLVGSGLLQRIIGYYSFIPEVGFY
                     LPDIPLFDITEILHKIMDINAVMIIPFSIKFYTHYLDKEHKILILSQEKTEAELQFLR
                     SQIQPHFLFNVLNDIYAMALLNKKETPDSILKLADLMRYVLQESTASKVPLEKEINYI
                     RNYIDLEKLRYNDLYAISFEIHGDSANKMITPLLLLPFIENAYKHSTTNDTGGAWIKI
                     RISLTNEVLTLEIMNSFDRNIPKVTQVSSGIGIQNVKKRLDILYQGKYTFYTENTAES
                     YKTILTINLDT"
     gene            2465..>2962
                     /locus_tag="PKN90_03790"
     CDS             2465..>2962
                     /locus_tag="PKN90_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ85433.1"
                     /translation="MKKIRCLLVDDEHLALEILELYCSKIDYLDVVGTCKNADEAAFF
                     LQHNKVDLLLLDIQMPRLNGMEWYKNLSSKPLVIFCTAFQHFAVESYEVNAIDYLLKP
                     FSLERFNKAIEKTKEIIEKKTNQETIEQSIFVRSDRKEIRVKIDDILFIQSMSNYYVV
                     TTTEKK"
BASE COUNT          994 a          523 c          464 g          981 t
ORIGIN      
        1 tacatccctc taaatttctc ttgacctata tacaccacat ttttcatctt aggaaaacgc
       61 tcgctgttga ggtatcctct ctgtgatgtt tttaattcgg gcaacatggt ataagtcata
      121 tccacatagt cgcattccca agtgccatca ataatacaca aggtatgcat gtcggaattt
      181 tgacacaaat attctaactc gtgttgcttg tagtttgtgt taaccgttac caatacggct
      241 cctattttgg atgtagcaaa taaaaaagtg agccaatcag gcacattggt tgcccaaatg
      301 cccacatggt gccctttttc aatgcctatt gccattaaac cctttgccaa gtcatttact
      361 cgctggtcaa aatcatgcca agtaaaacgt aagtttctgt cagaatatac gatacattct
      421 ttatttggtg tttctttagc ccagtactcg agccatcctc caattgttct gtctgataat
      481 tgcatatcta taattgctct aaaattagta ttcataaaga ttcgttgaat ggcactaaaa
      541 aggactatac acgactgcta aaattttagc tcctttttct ttatgtgcgt gtacatgatg
      601 ctctacaata gaatcgtaga aaatactatc tccttcttgc aaatgataca cagcttttcc
      661 gtaattaatc tcgatttcac cagataaaac ataaataaac tcttcccctt cgtgtgctga
      721 taaaataaaa tctttgctta cagatggttg gatatctata ataaaaggtt ccatatgacg
      781 accagctttt tgacctgcca aagaatagta ttccatatgc ttgcgggcac ctaccatatc
      841 gtttgaaaaa cttaaacttc cagatttttc acctttacga catacaactg gaccaatttc
      901 cgtattatca tccaaaaaag tacctaagcg aacacctaat gcacgtgcta tcttagtcaa
      961 tggagctaat gagggaagtt tttcgtcttc ttctattgaa cgaatttgct ctacagctaa
     1021 acccgaacgt tcagctaatt gttctacggt aatatcttta ccttctctaa ttgttttaat
     1081 ttttttacca acaaatgcat tgttttgtga catagattta attttttatg gttatcagat
     1141 taaaatatca tagtatatcg accacaaaaa tatacatttt atcttatcaa acaatatttt
     1201 tagcttattt acaaccaaaa gttatatttt tgcgttaatc aagtattttt ttccatccaa
     1261 ataaaaacga aaatagtcca gaagacatat tctaaaactt ctaactaaca gagaaaaata
     1321 cgtttacata aaaagaactt ctatttgcat aaatataaaa agaaaaatag agagaggtag
     1381 tacaatttgc aaaaaaactg tatatttgtt caactaaatc aattacttac atgcgactgc
     1441 taactggaaa aaatcgatgg ctactgcaca ttacatattg ggtaatggtg gtgttattat
     1501 ttacattttt ctggggcact cgttatggaa actattgggt gtgtttctac aacgagttgt
     1561 attttttacc tatcaaaatt gcagccactt actttggatt atattatctt ataccacaac
     1621 tttttaaacg caactattac cgcgttgtag ttgctggtag ttttatgctt gttggcagtg
     1681 gtcttttaca acgaattatt ggttactact catttatccc cgaagtagga ttttatttac
     1741 ccgacattcc tctgtttgac attactgaaa ttttacataa gataatggat ataaatgcgg
     1801 taatgattat tcctttttcc atcaaattct acacccatta tttagataaa gagcataaaa
     1861 tacttatttt atctcaggaa aaaacggaag cagagctaca atttttacgg agccaaatac
     1921 agccccattt tttattcaat gtgcttaacg atatttatgc aatggcatta ttgaataaaa
     1981 aagaaacacc cgattccatc ctaaaattag cggatttgat gcggtatgtt ttgcaagaat
     2041 ccactgctag taaagtgcca ttagaaaaag aaattaacta tatccgaaat tacattgatt
     2101 tagaaaaatt acgctataat gatttgtatg ctatttcctt tgaaatacac ggagatagtg
     2161 cgaataagat gattacccct cttttgctac taccatttat cgaaaatgct tataaacact
     2221 ctactacaaa tgatacaggt ggggcttgga taaagattcg tatcagccta acaaatgagg
     2281 tattaacgct tgaaattatg aacagttttg accgcaatat cccgaaagtt acgcaagttt
     2341 cgtctggtat tggcattcaa aatgtaaaaa aacgattgga tattttatat cagggtaagt
     2401 acacttttta cacagaaaac actgctgaga gttataaaac tatactaaca ataaacctag
     2461 atacatgaaa aagattcgtt gtttattggt agatgatgag catttggctt tagagatact
     2521 ggagctatat tgcagtaaga ttgattatct tgatgtagtt ggcacgtgta aaaatgccga
     2581 tgaagccgca ttttttttgc aacacaataa ggttgatttg ctattattag atattcaaat
     2641 gccaagacta aacggaatgg agtggtataa aaatctatca agcaaacctc ttgttatatt
     2701 ttgtacagcc tttcagcatt ttgccgtaga aagttacgaa gtaaatgcta ttgattattt
     2761 acttaaacca ttttcattgg aacgctttaa caaagcaata gaaaagacaa aggaaataat
     2821 tgagaaaaaa acgaatcaag aaaccataga acagtctatt tttgttcgtt ctgacagaaa
     2881 agaaattcgg gttaaaattg acgatatact atttattcaa agtatgagca actactatgt
     2941 agtaaccacg acagagaaaa aa
//
LOCUS       DAOPUR010000167         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_34681_length_2955_cov_3.358621, whole genome shotgun sequence.
ACCESSION   DAOPUR010000167 DAOPUR010000000
VERSION     DAOPUR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34681_length_2955_cov_3.358621"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..538)
                     /locus_tag="PKN90_03795"
     CDS             complement(95..538)
                     /locus_tag="PKN90_03795"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNZ85434.1"
                     /translation="MKKILFVCLGNICRSAAAEAVMKSVVNKIGRDKDFFIDSAGIIS
                     VHKGEPADNRMRKHAARRGYAVTSISRPVNYSDFEQFDIIVGMDNTNIDDLKDKAPSV
                     EAQKKIYKMTDFCERSQYDYVPDPYYGGQDGFELVLDILEILNVL"
     gene            complement(653..>2955)
                     /gene="priA"
                     /locus_tag="PKN90_03800"
     CDS             complement(653..>2955)
                     /gene="priA"
                     /locus_tag="PKN90_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNZ85435.1"
                     /translation="SYNGEFVLKEIIEILDDNQFVSNAQLAFWKWIASYYMCSLGEIM
                     VAAIPSVFRLESQSIIRLHPDFDERETYSADFSSKTQESIFQYIIQKKQLTIQQLIKQ
                     TGIKKPLYAVKQLVEQNMLTITHEVTEKFLPKYVTYVRLTWIAQHKIDSIFEEIKRAK
                     KQVHLLETFIDIAQCDDSTKPIPIEKSVLLQRASVSEAVLSELVKKQVLEVYKLPISR
                     FQETSSLTENKLVLSPDQERAYKEIVGYFETKKPVLLHGVTSSGKTEVYIQLIQDAVH
                     KDKQVLYLVPEIALTIQLLQRLEQAFGNKVGVFHSKYSDQERAEVYQNLLSPNPYKII
                     IGVRSSIFLPFQNLGLIIVDEEHEQSYKQVDPAPRYHARNAAIMLASIIQSNVLLGTA
                     TPAIETYYNVSVKKYAYVSLVSRYQNVLLPVIRVVDVADAYKKNRMKSMFSWFLIEKI
                     KEAIEQNQQVVLFQNRRGFSSHVECQQCGWVPKCMACDVSLTYHKFANYLSCHYCGYT
                     EQLPTQCAQCGNNQLKDRGFGTEKIEEELGKLFPGIRTARMDVDTTSKKNAYKRLIQA
                     FENHQIDVLIGTQMITKGLHFDNVSLVGILNADNLFNYPDFRANERAFQMLVQVSGRA
                     GRKQKQGTVVLQTFSPHNPIVNYIVNNDYHGFFCSETEERKVFNYPPFCRLIHIFVKD
                     SDKEIVDRVVNELACHLRRSFGNRVLGPDNPPVSRIQHIYIKKIIVKIELQSSFEKAK
                     EKVQNSIASLLHQYKKTRIVIDIDPL"
BASE COUNT          936 a          569 c          425 g         1025 t
ORIGIN      
        1 aagaatttcc gaatgaacca gactattata ttgcagaata cggaaatatg atgatttatt
       61 actcacagat tagaggaatg tatctagatg ctggttataa gacatttagt atttccagaa
      121 tatctaatac taattcaaat ccatcttgtc ctccataata tggatctggc acataatcat
      181 attgagaacg ttcacaaaaa tcagtcattt tataaatctt cttttgtgcc tccactgatg
      241 gagctttatc tttcaaatca tctatattgg tattatccat accaacaatt atatcaaatt
      301 gttcaaaatc gctataatta actggtctag aaatacttgt tacagcatac cctcggcggg
      361 ctgcgtgttt acgcattcga ttatctgcgg gctctccttt atgcactgat attatacctg
      421 ccgaatcaat aaaaaaatct ttatctcttc caattttgtt tacaacagac ttcattacag
      481 cttcagcggc agcagaacga cagatattac ccaaacatac aaataaaatt tttttcattt
      541 tgtatactaa ctttttacag agatagagat tgctactgca aaaataagga ataatactga
      601 attataacaa ttttaaacaa aatctttatc attcgcttaa ttcgttatat cactataaag
      661 gatctatatc aataacaatt ctagtttttt tatattgatg taaaagtgac gctatactat
      721 tttgcacctt ttcctttgct ttttcaaacg aactttgcaa ctctatcttt acaataattt
      781 ttttaatgta tatgtgttga attcttgata caggcggatt atctggacct aaaaccctat
      841 tgccaaaact acggcgtaaa tgacaagcta gttcgtttac cactctatct actatctcct
      901 tatcactatc tttaacaaag atgtgtatta atctgcaaaa tggtggataa ttaaatactt
      961 tccgctcttc ggtttcggaa caaaaaaaac catgataatc attatttaca atataattta
     1021 caatcggatt gtgtggagaa aatgtttgaa gcactacagt tccctgtttt tgtttccttc
     1081 cagctctacc gcttacttga acaagcatct gaaaagccct ttcgttggct ctaaaatcag
     1141 gataattaaa caaattatct gcatttaaaa tccctactaa actaacatta tcaaaatgca
     1201 aacccttcgt tatcatttgt gtcccgataa gcacatctat ttgatgattt tcaaacgcct
     1261 gaataagtct cttgtaagca ttttttttcg aagtagtatc tacatccatc cgagcagttc
     1321 gaatccctgg gaataattta cctaattcct cttcaatttt ttctgtacca aaacctctat
     1381 ctttcaactg attatttcca cactgggcac attgagttgg caactgttcc gtgtaaccac
     1441 aataatgaca cgataaataa ttagcaaatt tgtgatatgt aagacttaca tcacatgcca
     1501 tacactttgg cacccaacca cattgttgac attcaacatg ggatgaaaat cctctcctat
     1561 tctgaaacag taccacctgc tgattttgtt ctatagcctc tttaattttt tcaattaaga
     1621 accaagaaaa catcgatttc attctattct ttttatacgc atctgctaca tcaacaacac
     1681 gaattactgg caacaataca ttttgatacc tcgaaacaag agacacatag gcatattttt
     1741 ttacacttac attgtaatat gtttcaatag ctggagtggc agtccccagt aatacattac
     1801 tctggataat agaagccaac ataattgcag cattccgagc atggtatcgt ggagctggat
     1861 ctacttgttt ataagattgt tcgtgttctt catctacaat aataagacca agattttgaa
     1921 aaggcaaaaa aatagatgaa cgaactccaa taataatttt ataggggttt ggcgacagta
     1981 aattttgata tacctccgca cgttcttgat cagaatattt tgaatgaaac acccctacct
     2041 tgttaccaaa agcttgctcc aaacgttgca ataattgtat agtcaacgct atttctggca
     2101 ctaaatacaa cacttgttta tctttatgta cagcatcttg tattaattga atatatacct
     2161 ctgtttttcc acttgaggta actccatgta ataatactgg ttttttcgtt tcaaaatacc
     2221 caacaatctc tttgtatgct ctttcttgat cgggcgataa tacaagctta ttttcggtta
     2281 atgatgaagt ctcttgaaaa cgtgaaatag gtaatttata tacttctaat acctgttttt
     2341 ttaccaattc ggatagcaca gcttctgaaa cagaagctcg ttgaagcaaa acagactttt
     2401 ctatagggat gggctttgtg ctatcatcac attgagcgat atctataaag gtttcgagca
     2461 agtgcacttg tttttttgcc cgttttattt cttcaaaaat tgagtctatc ttatgttgag
     2521 ctatccatgt taatcgcaca tacgtaacat attttggtag aaatttttcg gttacttcat
     2581 gggtaatcgt caacatattt tgttctacca attgcttaac tgcatacaat ggttttttta
     2641 tacctgtttg tttaataagt tgctgtatcg taagctgctt tttttgtata atatactgga
     2701 aaatagattc ttgcgtttta gaagaaaaat cagcactgta cgtttctctt tcatcaaaat
     2761 caggatgcaa tcgtatgatt gattggcttt ctaatcgaaa aaccgaagga atagcagcca
     2821 ccatgatttc gcccaacgag cacatataat acgacgcaat ccatttccaa aatgccagtt
     2881 gagcattgct aacaaattga ttatcgtcta gaatctcaat tatttctttt agtacaaatt
     2941 caccattata cgaaa
//
LOCUS       DAOPUR010000168         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_34746_length_2950_cov_3.034542, whole genome shotgun sequence.
ACCESSION   DAOPUR010000168 DAOPUR010000000
VERSION     DAOPUR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34746_length_2950_cov_3.034542"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PKN90_03805"
     CDS             <1..1476
                     /locus_tag="PKN90_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNZ85436.1"
                     /translation="SVKLVSETGVGTIAAGVAKAKADMITISGCEGGTGASPASSIRH
                     AGLPSEIGIAETQQTLIINNLRGNVSLQVDGQLKTGRDIVIQAMLGAEEFGFATSALI
                     VLGCVMMRKCHVNTCPMGVATQNEELRKRFIGRSEYLINFFTFLAQNVREHLAELGYT
                     SLDEIIGRSDLLIQKTETNNPKIEKIDLSRLLYRPKEADYNAIRKISATNHQIEPNLN
                     TQLVEDAKETIASGKSIQKDYIIKNINRSIGTTLAGEIAKKYAEDGLPEGTIQFAFTG
                     SAGQSFGAFLTKGMQFTLYGDANDYLGKGLSGGTIIVVPEKESLFEPHKNIIAGNTLL
                     YGATTGEVYINGAVGERFCVRNSGAIAVVEGTGDHCCEYMTGGRTVVLGTTGRNFAAG
                     MSGGVAYVWNKNGDFDYFCNMEMVELSLIENAEDNKELVGCIAKHYDYTKSPLAKEML
                     DNWDIYQQQFIKVMPIEYKKVLHDEKVRQLQAKINDVQRDY"
     gene            1513..2802
                     /locus_tag="PKN90_03810"
     CDS             1513..2802
                     /locus_tag="PKN90_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNZ85437.1"
                     /translation="MGNPKAFYTIKRKDAGYRPVHERVADYGEVEQTLNEEDRQLQAS
                     RCMDCGIPFCTWSCPLGNIQPEWQHLLYLGKWREAFEVLESTDDFSEFTGRVCPAPCE
                     HGCVLNIHKEPVTIRENEVSIIEHAFAEGYVVPKPPKTRTGKKVAVIGSGPTGLACAN
                     QLNKRGHLVTVYEKDEAIGGLLRFGIPDFKLNKKIIDRRLAILEEEGIKFVCNAYIGV
                     NVSIEKLVEEYDAICIAIGAGQPRDIQVEGRNLKGIHFALDYLQQQNRVVSEAVIPEE
                     ERIFAKGKHVLVIGGGDTGSDCVGTANRQGAVKITQIEIMPKPPVSDNPDSPWPYYQF
                     VLKTSSSHKEGCERRWALDTRKFIGENGQVTAVEVEEVKWEKDQSGRLQLIRTGNVDV
                     IQADLVFLAMGFVHPVQEGLVEQLNLAVDGRKNIACR"
BASE COUNT         1004 a          470 c          652 g          824 t
ORIGIN      
        1 agtgtaaaat tagtttccga aacaggtgtt ggtactattg ctgcaggtgt tgccaaagca
       61 aaagctgata tgataacgat tagtggatgc gaaggtggaa cgggagcaag tcctgctagc
      121 tctatacgtc atgctgggtt accttctgaa atagggattg ccgaaacaca acaaacgttg
      181 attatcaata atttacgtgg caatgtatcc ttacaagtag atggtcaatt gaaaacagga
      241 cgcgacattg tcattcaagc aatgctaggt gccgaagagt ttggatttgc tactagtgca
      301 ctcattgtat taggttgtgt gatgatgcgc aaatgtcacg taaacacttg tccaatgggt
      361 gtagccacac aaaacgaaga attgcgtaaa cgatttatcg gtcgttcgga atatttaatc
      421 aatttcttta cattcttggc tcaaaatgtt cgtgagcatt tggctgaatt aggatatacc
      481 agtttagatg aaataattgg acgttcagat ttattaattc aaaaaactga aacgaacaat
      541 ccaaaaattg aaaaaattga tttatctcgc ttattatatc gccctaaaga agctgattat
      601 aatgctatta gaaaaatatc agcaaccaac catcagattg aaccaaattt aaatacacag
      661 ttggtggaag atgcgaaaga aacaattgca tcaggaaaat ccattcaaaa agattacatc
      721 atcaaaaata ttaatcgttc tataggcacc actttagctg gtgaaatagc aaaaaaatat
      781 gcagaagatg gacttccaga aggcactatt caatttgcat ttactggatc tgctggacaa
      841 agttttggag ctttcttaac taaaggtatg caatttactc tgtatggaga tgcgaatgat
      901 tatttaggaa aaggattgtc gggcggtact attattgtag ttcctgaaaa agaaagtttg
      961 ttcgaacctc acaaaaacat cattgctgga aatacattat tgtatggggc aaccactggc
     1021 gaagtatata ttaatggtgc tgtgggcgaa cgtttttgtg tgcgtaatag tggtgcaata
     1081 gcggttgtag aaggaacagg cgatcactgt tgcgaatata tgactggtgg tagaactgtg
     1141 gtactcggta caacaggtag aaactttgct gctggtatga gtggtggtgt ggcttatgta
     1201 tggaataaaa atggagattt tgattatttc tgtaatatgg aaatggtaga attatccctc
     1261 atagaaaatg cagaagacaa taaagaatta gttggttgta ttgctaaaca ttatgattat
     1321 accaaaagtc cattagcaaa agaaatgcta gataattggg atatttacca acaacaattt
     1381 atcaaggtta tgcctattga gtacaaaaaa gtacttcacg atgaaaaagt gcgtcaatta
     1441 caagcaaaaa ttaacgatgt gcaacgtgat tattaattta ggcaattcat acaacattta
     1501 agttttacaa ttatgggaaa tccaaaagca ttttatacaa tcaaacgaaa agacgctggc
     1561 tatagacctg tacacgaacg tgtagcagac tatggcgagg tagagcagac tttaaacgaa
     1621 gaagatcgtc agttacaagc ctctaggtgt atggattgtg gtataccatt ttgcacgtgg
     1681 agttgtccat taggcaatat ccaaccagaa tggcaacatt tattatattt aggaaaatgg
     1741 agagaagctt ttgaagtatt agaaagcacc gatgattttt ctgaatttac aggtagagtg
     1801 tgtcctgcac catgcgagca tggatgtgtg cttaatatac acaaagagcc cgttaccatt
     1861 cgcgaaaacg aagtatccat tatcgaacat gcctttgcag aaggctatgt agtgccaaaa
     1921 ccacctaaaa cccgtacagg gaaaaaagta gccgttattg gttcaggtcc tactggttta
     1981 gcatgtgcta accaattaaa caaaagaggt catttagtta ctgtgtacga aaaagatgaa
     2041 gcaataggtg gattattacg gtttggcatt cctgatttca aactaaataa aaaaattatt
     2101 gatcgccgat tggctattct tgaagaagaa ggtatcaaat ttgtgtgcaa cgcatatata
     2161 ggagtaaatg tatccatcga aaaattagta gaagaatacg atgctatttg tattgctata
     2221 ggtgctggtc aaccgcgcga tattcaagta gaaggtcgca acctaaaagg tatacacttt
     2281 gcccttgact atttgcaaca acaaaacaga gtagtatctg aagccgtaat accagaagaa
     2341 gaaagaattt ttgctaaggg aaagcatgtg ctagtaattg gtggtggtga tacaggttct
     2401 gactgtgtag gaacagccaa cagacaaggt gctgtaaaaa ttacacagat agaaattatg
     2461 ccaaaacctc ctgttagcga caatcccgat tcgccatggc cctattacca atttgtacta
     2521 aaaacatcaa gctctcacaa agaaggttgt gaacgcagat gggcactaga tacacgcaaa
     2581 tttattggcg aaaatggtca agttacagcc gtagaagtag aagaagtgaa atgggaaaag
     2641 gaccaaagcg gacgtttgca attaatcaga acaggtaatg ttgacgtaat acaagccgat
     2701 ttagtatttt tagcaatggg ttttgtgcat cccgtacaag aaggtttggt tgaacaactt
     2761 aacttagctg tagatggcag aaaaaatatt gcttgtcgat gatgaggttt ctcttcagga
     2821 aacattaagt tacaagttaa aaaaagaagg ctatgaagtt gaagtagccg gagacggtct
     2881 gactgctttg gagttggctc gctcgaccaa cccagatctt atcattttgg atgtgatgtt
     2941 gccaggcatg
//
LOCUS       DAOPUR010000169         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_34975_length_2935_cov_2.684722, whole genome shotgun sequence.
ACCESSION   DAOPUR010000169 DAOPUR010000000
VERSION     DAOPUR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34975_length_2935_cov_2.684722"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKN90_03815"
     CDS             complement(<1..602)
                     /locus_tag="PKN90_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNZ85438.1"
                     /translation="MNTTKYKMLVLDLDDTLLRDDYSISERTINALIATQQMGVKVVL
                     ASGRPTLAMTQYAKMLQQHDFDSYMISFNGAVVSSTRTNEVIFETSLTQSEIHSLYDF
                     SVEHGVHIITYSDKGIVSETHSEYIDVELKLTGIPHYKVPSFKDEVSYSAIKCILLEH
                     PDYLKQVELVLKSQRTDLSVSRSKPFFLEVMPKGIDKAAS"
     gene            complement(609..1874)
                     /locus_tag="PKN90_03820"
     CDS             complement(609..1874)
                     /locus_tag="PKN90_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ85439.1"
                     /translation="MKNKPISPWAWIPSLYFGQGIPYVVAATTFSLIMYKNMGISNAD
                     AALYTSWLYLPWVLKPFWSPFVDLIKTKRWWILLMQFVIGIALAGVAFAIPLPYFLQS
                     TLAILWLIAFSSATHDIAADGFYMLALDTHEQSLFVGIRSTFYRVAMIVGQGALVMLA
                     GYLETTMPSIAIAWSITFYGIAVFFFALVLYHWFVLPKPSTDIVRNDKRVVTDFFATF
                     GAFFTKKGIWLSIVFILLYRLGEAQLVKIASPFLLDPRSVGGLGLTTSEVGFVYGTIG
                     VIALTLGGILGGIVAAKDGLKHWLWIMVIAMNLPNLVYVYFAYMQPDNFYIIASGVAI
                     EQFGYGFGFTAFMLYLIYIAKGEFQTAHYAIATGFMALGMMLPGMFAGILQEYMGYFP
                     FFVWVCICTIPGFLLIPFLKIDPSFGKKE"
     gene            complement(2058..>2935)
                     /locus_tag="PKN90_03825"
     CDS             complement(2058..>2935)
                     /locus_tag="PKN90_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNZ85440.1"
                     /translation="RSWLFAQIQQVCSSQAETLSIEGNTMIKWYDHHNHLLFDVCADD
                     HCQRYQGVAKVTTNQVQKAIEETYGEVLVYQNNLCDARFSKCCGGVTEEYATCWENSQ
                     VAYLQSIVDEKQEEKKLDLRTESAITSWIRSSPTVFCNTSDAHILSQILPHFDQETTD
                     FFRWKKVYSQHELSTLVHKRSGIDFGVIHDIRPIKRGKSGRLEIIEIVGTTRTMRIGK
                     ELEIRKWLSDTHLYSSAFIVERDDERNFIFYGAGWGHGVGLCQIGAAVMSEQGYSYKE
                     ILQHYYTGVEILSVY"
BASE COUNT          963 a          626 c          469 g          877 t
ORIGIN      
        1 aaactagccg ccttatctat tcctttgggc atcacttcta aaaaaaacgg cttagaacga
       61 gacacactca aatctgtacg ttgcgattta agtaccaatt ccacttgttt taaataatct
      121 ggatgctcta acaaaataca ttttatagcc gaatagctaa cttcatcttt aaagctaggc
      181 actttgtaat gaggtattcc tgtcagtttt agttctacat caatatactc ggagtgagtt
      241 tcgctcacaa tacccttgtc tgaatacgta ataatatgta caccatgctc aacgctaaaa
      301 tcgtataaac tgtggatttc cgattgggtt aaacttgttt caaaaattac ttcattcgtt
      361 ctggtagaac taacgactgc tccattaaac gaaatcatat aggagtcaaa atcgtgttgt
      421 tgtaacatct tagcatattg agtcatagct aaggttggtc tgccagaggc taatacaacc
      481 tttaccccca tctgttgggt agcaataaga gcattgatgg ttctctctga aatactataa
      541 tcgtctcgaa gtaaggtatc atctaagtcg agcaccaaca ttttatactt agttgtgttc
      601 atttcttgtt attctttctt cccaaatgat ggatcaattt tcaaaaatgg tattaataaa
      661 aacccaggaa tggtacaaat acacacccat acaaaaaatg ggaaataacc catatactct
      721 tgcaaaattc ctgcaaacat gcccggcaac atcattccta aagccataaa accagtagct
      781 atagcataat gtgccgtttg aaattctcct ttggcaatat atataaggta cagcataaag
      841 gcagtgaaac caaatccata gccaaattgt tctatagcca cacctgaagc tatgatataa
      901 aaattgtcag gttgcatata agcaaaatac acatacacta ggttgggtaa gttcatggct
      961 ataaccatta tccacaacca atgttttaat ccatctttag ctgccacaat acctcccaaa
     1021 atacccccta acgtaagtgc aattacacct atggtaccat acacaaaacc cacttcagag
     1081 gtagttaatc ctaagccccc aacactacgt gggtcaagta aaaatggaga tgcaatttta
     1141 accaattgag cttctcccaa gcgatagagc aatataaaca caatggacaa ccaaatgcct
     1201 tttttagtga aaaaagcacc aaatgtagcg aaaaaatctg taaccacacg tttatcattt
     1261 ctaacgatat ccgttgatgg ttttggtaat acaaaccaat gataaagaac caatgcaaag
     1321 aaaaaaacag ctattccata aaatgtaatg ctccatgcaa tggcgataga gggcatggtt
     1381 gtttctaaat aaccagcaag cattaccaag gctccttgcc ccacaatcat cgcaacacga
     1441 taaaaggtac ttcgaatgcc gacaaaaagg gattgctcgt gcgtatctaa ggctaacata
     1501 taaaatccat cagctgctat atcgtgcgta gctgaactaa aggctattaa ccataatata
     1561 gccaatgtac tttgcaaaaa atagggcaag ggaatggcga aggcaacgcc agccaaagct
     1621 attcctatca caaactgcat aagcaatatc caccagcgtt ttgttttgat taaatcgaca
     1681 aacgggctcc aaaagggttt aagtacccaa ggcaaataca accagctagt atataaggct
     1741 gcatcggcat ttgaaatacc catatttttg tacataatga gagaaaaagt agtggcagct
     1801 actacataag gaattccttg tccaaaatac aggctaggaa tccatgccca tggtgaaata
     1861 ggtttatttt tcatatatat ttttttatct actagtaatt ggcgttaatt aattttcttc
     1921 ttcgtgtaag attctttcac agagtgacac agaggtttca cagaggcaca cggagtaata
     1981 tgtttttctc tcagtgtaac ttcgtgtttc cttcgtgttc ttcgtgaaat aatagtatac
     2041 aaagtgtaac aaaaagacta atatacggaa agtatttcca ctcctgtata ataatgctgc
     2101 aaaatttctt tatacgaata tccttgttca ctcattacag ctgctcctat ctggcaaaga
     2161 cctacaccat gcccccagcc tgccccataa aaaataaaat tcctttcatc atctcgctca
     2221 acgatgaatg ctgaactata taaatgcgta tctgataacc acttacgaat ttctaattct
     2281 tttccaattc gcatagttct ggttgttcct acaatctcaa taatttctaa tcgacccgat
     2341 tttccacgtt taattggacg tatatcgtgt ataactccaa aatcaatacc cgatcgtttg
     2401 tgtactaatg tcgataattc gtgttgacta tacactttct tccacctaaa aaagtctgta
     2461 gtttcctgat cgaaatgagg cagaatttga gacaaaatat gagcatcaga agtattacaa
     2521 aatacagttg gtgatgatct tatccaagat gttattgctg actcagtacg taagtccagc
     2581 tttttctctt cctgtttttc atctactatt gattgtaaat aagcaacttg actgttttcc
     2641 caacacgtag catactcttc ggttacgcct ccacaacatt ttgaaaagcg tgcatcacac
     2701 aagttatttt gatatactaa cacctctcca tatgtttctt caatagcttt ttgcacctga
     2761 tttgtagtta ccttcgctac gccctgatac cgttggcaat gatcgtcagc acacacatca
     2821 aatagtaaat gattgtgatg atcataccat tttatcatcg tattcccctc aatactcaaa
     2881 gtttcagcct gacttgaaca gacttgttgt atttgtgcaa aaagccaact acgag
//
LOCUS       DAOPUR010000170         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_35004_length_2933_cov_3.727589, whole genome shotgun sequence.
ACCESSION   DAOPUR010000170 DAOPUR010000000
VERSION     DAOPUR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35004_length_2933_cov_3.727589"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PKN90_03830"
     CDS             <1..683
                     /locus_tag="PKN90_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85441.1"
                     /translation="LIYAAISFTHTPQEQVCSTIHIFKIDSVDYNFIKRKEIEKQLTD
                     NNIYPIGRKIDFAFTHAIEEKIKEMNYVKRVECYRTNHGDVVIRIWQRQPVVRIITPF
                     YSYYIDQDRKKMPISKDFTVILPIVSGNVTESFAKNELFDFVTYITNDSFWNAQIDQI
                     YIKENNKIELVPKVGLYTITLGTLDDYETKLKKLYAFYKTYPEFALTDTYSNINIEYK
                     KLLYATKK"
     gene            694..2001
                     /gene="ftsA"
                     /locus_tag="PKN90_03835"
     CDS             694..2001
                     /gene="ftsA"
                     /locus_tag="PKN90_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNZ85442.1"
                     /translation="MNTSTPYILAFDLGTTKIIGAVAQKTTEGKLSIVAYEEEETKPT
                     TIKQGAIFNVADTAFIINSIVTKLRNKTKLNLTKAYIGIGGRYLRCKENTTKRTLGYN
                     EIVEQNLLDKLYQENFKLQSTEFDILDVITQEYLVDDEWVADPAGIACTQLEGRYKII
                     YTKSEINTGINKVKELLAEKSQLEIISTFPSPIATANGLISDDEKELGCMIIDFGGST
                     TSYSVFTKNYLREIGVIPLGAKTITNDIRELNLTEPQAEKLKQKLGNAMATLEVPDKR
                     IIIAKAAHEQEEKSIQSTFLAQIIEARASEILNFIITPLKQKGYMKQLGAGIIITGGG
                     AQLKNMRALVQSLSGVDVRTTVGVHLLTEDSISDDFKKPSYSQIIGLLSMGTENCVSE
                     TIEEKFIPSKKTKKTGKNLFVRFADSASQRLTDLFTDNSDTIQ"
     gene            2026..>2933
                     /gene="ftsZ"
                     /locus_tag="PKN90_03840"
     CDS             2026..>2933
                     /gene="ftsZ"
                     /locus_tag="PKN90_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNZ85443.1"
                     /translation="MVNFDFSTNADTIIKVIGVGGGGGNAVNHMFNQGIKDVTFAVCN
                     TDYQDLRRSPIPIKIQLGDKGLGAGNIPEVAREAALETMDKIEELLDDSTKMVFITAG
                     MGGGTGTGAAPVIAEIAKNKSILTVGIVTIPFEFEGEKKIQKALNGVREMSKHVDALL
                     VINNERLIDIFPDLKMSTAFAKADNILTDAAKGIAEIITIPGYINVDFADVQTILKEG
                     GFAIMNTGFASGEKRVSKAIHDALNSPLLNNNDIKGAKRILLHVYTSKENELDMTETI
                     EIKHFLNNIAKDAEVIAMGRDIAMQVA"
BASE COUNT         1127 a          473 c          519 g          814 t
ORIGIN      
        1 atttaattta cgcagcaatc tcctttaccc atacaccaca agagcaagtg tgtagtacaa
       61 tacatatttt caaaattgat agtgttgatt ataattttat taaacgcaaa gaaattgaaa
      121 aacaattaac agataacaat atatatccga taggacgaaa aattgatttc gcatttacac
      181 atgctattga agaaaaaatc aaagagatga actatgtaaa acgggtagag tgttatcgaa
      241 ccaatcatgg agatgtggtc atacgcatat ggcaacgcca acctgttgtt cgtattatta
      301 caccattcta ttcttattac attgatcaag acagaaaaaa gatgccaata tctaaagatt
      361 tcacggttat attgcctatc gtatctggta atgtaacaga aagttttgcc aaaaacgaac
      421 tctttgattt tgtaacatat atcaccaacg attcattttg gaatgctcaa attgatcaaa
      481 tatacatcaa ggaaaataat aaaattgaat tagtgccaaa agtaggatta tacactatta
      541 cattaggaac acttgatgat tatgaaacca aattaaaaaa actgtatgca ttttataaaa
      601 catatcctga atttgcttta acagacactt actcaaatat caatattgaa tacaagaaat
      661 tactatatgc aactaaaaaa taattccaat actatgaaca catctactcc ttacatccta
      721 gcttttgatt tgggtacaac caaaataatt ggtgcagtag ctcaaaaaac aaccgaagga
      781 aagctctcaa ttgtggcata cgaagaagag gaaaccaagc caactaccat taaacaaggg
      841 gctatattta atgtggcaga cacagctttt attatcaata gtattgttac aaaattacgt
      901 aacaagacta agttaaacct aaccaaagca tatattggta taggtggcag gtatttgcgt
      961 tgcaaagaaa acacaacaaa acgtacatta ggttataacg aaattgtaga acaaaatttg
     1021 cttgataaat tgtatcaaga gaactttaaa ctacaatcta ctgagtttga tatcttagat
     1081 gtaatcactc aggaatattt agttgatgat gagtgggtag cagatcctgc aggtatagca
     1141 tgcacacaat tagaaggacg ttacaaaatt atttatacaa aaagcgaaat taatacagga
     1201 atcaacaaag taaaagaact cttggcagaa aaatcacaat tagaaataat tagcacattt
     1261 ccctctccca ttgctacagc aaacggactt atttcggatg acgaaaaaga acttggctgt
     1321 atgataattg attttggagg gagtacaact tcgtatagtg tatttacaaa aaattacctc
     1381 agagaaatag gtgtcattcc attgggagct aaaacaatta caaacgatat aagagaactt
     1441 aatttaacag aacctcaagc cgaaaaacta aagcaaaaac taggcaatgc aatggcaact
     1501 ttagaggtgc ctgataagcg tattattatc gctaaagcag ctcacgaaca agaagaaaaa
     1561 tcgatacaaa gcacattttt agctcaaatt atcgaagcaa gggcaagtga aattttaaat
     1621 ttcatcatta ccccattaaa acaaaaagga tacatgaaac aactcggtgc cggtatcatt
     1681 attactggag gaggagctca actcaagaat atgagagctt tggtacaatc gctgagtggt
     1741 gtggacgttc gtactactgt tggtgtacat ttacttacag aagattctat ttcggatgat
     1801 tttaaaaaac catcttacag tcaaattatt ggcttgttat caatgggaac agaaaattgc
     1861 gtatcagaaa ctattgaaga aaaatttata ccatcgaaaa aaacaaaaaa aactgggaaa
     1921 aatttattcg tacgttttgc cgatagtgca tcacaacgat taacagattt atttaccgac
     1981 aatagtgata caattcaata agacaacaaa ctaaaaataa ctatcatggt aaattttgat
     2041 ttttcaacaa atgctgatac aattataaaa gtaattggtg taggtggagg tggtggcaat
     2101 gctgtaaatc atatgtttaa tcaaggtatt aaggacgtta catttgctgt atgtaatacc
     2161 gattaccaag atttaagacg ctctccaatc cctattaaaa tacaactagg agacaaagga
     2221 ctgggagcgg gtaatatacc agaggtagct cgcgaagcag cccttgaaac catggacaaa
     2281 atcgaagaac tattggatga tagtactaaa atggtattta ttacggcagg aatgggagga
     2341 ggaacaggta ctggtgctgc ccctgttatc gcagaaatag ccaaaaacaa atctatctta
     2401 acagtaggaa ttgttacaat cccttttgaa tttgaaggag aaaaaaagat tcagaaagca
     2461 ctgaatgggg ttcgcgaaat gagcaaacat gtagatgctc tgttagtgat taataacgaa
     2521 cgtcttattg atatttttcc agatttaaaa atgtcaacag cctttgccaa ggctgataat
     2581 atcctaacag atgcagccaa gggaattgcc gagataatta ccattccagg atatattaat
     2641 gtggactttg ctgatgtaca aactatttta aaagaaggtg gttttgctat tatgaataca
     2701 gggtttgcta gtggcgaaaa acgtgtaagt aaagctattc acgatgcatt gaactctcct
     2761 cttctcaata ataacgatat aaaaggagcc aaacgcatac tattacatgt atacacctcc
     2821 aaagaaaatg aactcgacat gactgaaacc attgagatta aacatttctt aaacaatatt
     2881 gctaaagatg cagaagttat agctatggga agagatatag ctatgcaagt tgc
//
LOCUS       DAOPUR010000171         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_35054_length_2930_cov_3.393739, whole genome shotgun sequence.
ACCESSION   DAOPUR010000171 DAOPUR010000000
VERSION     DAOPUR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35054_length_2930_cov_3.393739"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKN90_03845"
     CDS             <1..788
                     /locus_tag="PKN90_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ85444.1"
                     /translation="QLIDATYSRIQFTPDLLPADVVGTMIYSQKKEQFEVKKGPIFAN
                     FILADEINRAPAKVQSALLEAMQERQVTLAETTHKLPSPFLVLATQNPIEQEGTYPLP
                     EAQVDRFMLKVVVSYPKKEEERLIIRQNIGPEQKALVPVLTIKDILDARSIVKEIYID
                     EKIEKYILDIVFATRNPEEYGLAKLKDMIAFGVSPRASINLALAARAYAFIKHRGYVV
                     PEDIRAVCYDVLRHRIGLSYEAEATNLTPEDIISEIINAVEVP"
     gene            807..1676
                     /locus_tag="PKN90_03850"
     CDS             807..1676
                     /locus_tag="PKN90_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNZ85445.1"
                     /translation="METSEVIKRVRQVEIKTKGLSQNIFAGQYHTAFKGRGMAFSEVR
                     EYQYGDDIRNIDWNVTARFNHPYIKIFEEERELTVMLLIDVSGSREFGSVDKTKKNII
                     TEVAATLAFSAIQNNDKIGVLFFSDRIEKFIPPQKGKKHILHIIRELIDLHPKSRKTD
                     ISMVLKYFSNAIKKKCTAFILSDFIDTNDFEQAMLLANKKHDLVALQIYDKRETELPN
                     VGLLKIKDAETGEIVLVDSSNKAIRDLYQSVWNKKQYKLNQVFNRCRVDNVSIDTTED
                     YVKSLLKLFRLRS"
     gene            1680..2627
                     /locus_tag="PKN90_03855"
     CDS             1680..2627
                     /locus_tag="PKN90_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85446.1"
                     /translation="MYLNKFSWLIIFFFLSVPIAYCQTVTIDAELDSSTIIIGDQAIL
                     HIQITKPVAESIVIPQFTDTLVKGIDIISITKIDTTLVDDKQNIQFSIYLTSFEPGDY
                     TIPAIKFVGENDTYQTPLFPLLVKPFNINPDDEKLNDIKPILQMPFSWKTFFKWTAIV
                     LLVLALVAGIVYILVKYVFKKKIPLVKSKEEIIPPHIIAIQELDKIKAEKLWQQGKVK
                     EYYSAITDVIRVYLERRFDIPALEMTTHELVKLISTNPETLPIKDTLSELLHLSDIVK
                     FAKYIPLNSDNDLSLIHSYFIIDKTKIEPITEPTSEKDM"
     gene            2629..>2930
                     /locus_tag="PKN90_03860"
     CDS             2629..>2930
                     /locus_tag="PKN90_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ85447.1"
                     /translation="MVFQHPYILFLLFLLIPYIAWYIWKRRTLTPEIQFPTTGVLAQA
                     PKTPRMLLRHVPFVLRCLSVASIIFVIAQPQKRDSWKNETTEGIDMMLVMDISGSML"
BASE COUNT         1049 a          434 c          511 g          936 t
ORIGIN      
        1 cacaattaat tgatgctaca tacagtcgca ttcagtttac accagatttg ttacctgctg
       61 acgtagtggg tactatgatt tatagtcaaa agaaagagca atttgaagta aagaaaggac
      121 caatttttgc taattttatt ttagcagacg aaataaaccg tgctccagca aaagtgcaaa
      181 gtgcactact tgaggctatg caagaaagac aagttacgtt ggctgaaaca acacacaagc
      241 tgccttctcc ttttttggta ttagctactc aaaatcctat tgaacaagaa ggaacatacc
      301 ctttgccaga agctcaggta gatagattta tgttaaaagt cgtagtgtcg tatcctaaaa
      361 aagaagaaga aagattaatt attagacaaa atattggacc agagcaaaaa gcccttgtcc
      421 ctgtgttaac tattaaggat attcttgatg cccgttctat tgtaaaggag atatatattg
      481 acgagaaaat tgaaaaatat attttagata ttgtttttgc aacgcgtaat ccagaagagt
      541 atggtcttgc aaaattgaaa gacatgattg catttggcgt ttcgccacgt gcgtctatta
      601 atttagcatt agcagctagg gcatatgcat ttatcaaaca tcgtggatat gtagtgccag
      661 aagatattcg agctgtgtgt tatgatgtat tacgacacag aataggatta agttatgaag
      721 cagaagccac aaatttaact cctgaagata ttattagcga aataataaat gcagttgagg
      781 taccttaatc tatcaacatc gaattgatgg aaacaagtga agtaataaaa cgtgtacgac
      841 aggtagagat aaaaaccaaa ggcttatctc aaaatatttt tgcagggcag tatcatacag
      901 ctttcaaggg taggggtatg gctttttcag aagtaagaga atatcagtat ggcgatgata
      961 tacgtaatat tgattggaac gtaacagccc gatttaatca tccatatata aaaatttttg
     1021 aagaagagag agaattaacc gtaatgttgc ttattgatgt gtcgggtagc cgtgaatttg
     1081 ggtctgtaga caaaaccaaa aaaaatatta ttaccgaagt agctgctaca cttgcttttt
     1141 ctgctattca gaataatgat aaaattgggg tattattttt ttcagataga attgaaaaat
     1201 ttatcccgcc acaaaaagga aagaaacata ttttacatat tattcgcgaa ttaattgatt
     1261 tacatcctaa gagccgtaaa actgatattt cgatggtgtt gaaatatttc tctaatgcga
     1321 ttaagaaaaa atgtaccgct tttattttgt ccgattttat tgatacaaat gattttgaac
     1381 aagccatgtt attggcaaac aaaaaacatg atttggtagc attgcagata tatgataaaa
     1441 gggaaacaga attacccaat gtgggcttgc ttaaaataaa agatgccgaa actggagaaa
     1501 ttgtactagt tgattcgtca aataaagcca taagagacct atatcaatcc gtttggaata
     1561 aaaaacaata taagcttaat caggtattta atagatgtag agtagataat gtttccattg
     1621 ataccacaga agattatgta aaatccttac ttaaattatt ccgtttaaga tcttaatgta
     1681 tgtatttaaa taagtttagt tggttgatta tatttttctt tttgagtgtg ccaatagcat
     1741 attgtcaaac agttacaatt gatgctgagt tggattcttc tactattata attggagatc
     1801 aagctattct acatatccag ataactaagc cagtagcaga atcaattgtc atccctcaat
     1861 ttacggatac tctggtaaaa ggtatcgata ttattagtat aactaaaata gatacaacac
     1921 ttgtagatga taagcaaaat attcagtttt caatttattt aacttctttt gaaccaggag
     1981 attatactat acctgctatt aaatttgtag gcgaaaacga tacgtaccaa acgccattat
     2041 ttcctttgct tgtaaaacct ttcaacataa atcctgatga tgaaaaattg aatgatataa
     2101 agcctatttt acagatgcca tttagttgga aaacattttt taaatggaca gccattgtat
     2161 tgcttgttct tgctttagta gcaggtatag tatacatcct tgtaaaatac gttttcaaga
     2221 aaaaaattcc gttagttaaa tctaaagaag aaattattcc tcctcatatc attgccatac
     2281 aagaattgga taaaattaaa gcagaaaaat tatggcaaca aggcaaagta aaagaatatt
     2341 attcagcaat tacagatgtg ataagggtat atctcgaaag gcgatttgat attccagcat
     2401 tggagatgac aacacatgaa ttagtaaaat taatttcaac taatcctgaa acactaccta
     2461 taaaagatac gttgagcgaa ttattgcatt tatctgatat agttaagttt gctaaatata
     2521 tacctcttaa cagtgataat gatttgagtt taatacacag ttattttata attgataaaa
     2581 caaaaattga accaataaca gaaccaactt cagaaaaaga catgtaatat ggtatttcaa
     2641 catccttata tattattttt attattttta cttatacctt acattgcttg gtatatatgg
     2701 aaacgtcgta cattaactcc tgaaatacag tttcctacca caggtgtttt agctcaagca
     2761 ccaaaaacgc cacgaatgtt attgcgtcat gttccttttg ttttacgttg cttatctgtt
     2821 gctagtatta tttttgtaat cgctcaaccg caaaaacgag atagttggaa aaacgaaact
     2881 acagagggaa tagatatgat gcttgttatg gatatttccg gaagtatgct
//
LOCUS       DAOPUR010000172         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_35420_length_2907_cov_3.756662, whole genome shotgun sequence.
ACCESSION   DAOPUR010000172 DAOPUR010000000
VERSION     DAOPUR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35420_length_2907_cov_3.756662"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKN90_03865"
     CDS             <1..548
                     /locus_tag="PKN90_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNZ85448.1"
                     /translation="VFGESQAQELVPKAQLLPKDIEWHFIGHLQTNKVKYIIPYVSLI
                     HAVDSLKLVQEINKQALQQHRIMDCLVQIRIAQEETKYGMTLEESASMLHYISVNNFT
                     AIRICGVMGLATDTEDQNQVTKEFQYLTYCFHTLKNTFFQTSLYFSEISMGMTHDYPL
                     AIEQGSTLIRVGSGIFGERVY"
     gene            696..>2907
                     /gene="ppdK"
                     /locus_tag="PKN90_03870"
     CDS             696..>2907
                     /gene="ppdK"
                     /locus_tag="PKN90_03870"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNZ85449.1"
                     /translation="MDKKRVYFFGNKTAEGNGSMKELLGGKGANLADMNLIGVPVPPG
                     MTITTEVCNEYFLIGQEEVIKTIKPEVEKAMKQIEDAVAGPKFGDNKNPLLVSVRSGA
                     RASMPGMMDTILNLGLNDEAVETIAQKSGNPRFAWDSYRRFVQMYGDVVLGMKPESKQ
                     DEDPFEVIIEKMKHEKKVELDTQLTTEDLKELVAKFKKAVKDVTGKDFPVSPWEQLWG
                     AVMAVFNSWMNERAILYRKMNKIPAEWGTAVNVQAMVFGNMGNNSATGVAFTRDASTG
                     EDIFNGEYLINAQGEDVVAGIRTPQEITIEGSRRWAKMQGISEADRLAKYPSLEETMP
                     SAYADLNAIQQKLEDHYTDMQDIEFTIQDGKLWMLQTRNAKRTGAAMVRVAMEMVKQG
                     IIDEKTALLRCEPEKLDELLHPVFDTKALAQAKALSQGLPASPGAATGQVVFFAEDAE
                     AWAAEGKKTILCRVETSPEDLKGMYAAEGILTARGGMTSHAAVVARGMGKCCISAANG
                     LVIDYKTRTMTVGGATIKEGEWVSLNGTTGILYAGKVETKAAELSGDFAELMKIADKY
                     ARLQVRTNADTPHDAQVARNFGAQGIGLTRTEHMFFEGDKIKAMREMILSEDEAGRRK
                     ALVKLLPLQREDFEGIFEAMNGLPVTVRLLDPPLHEFVPHDPKGQLEMAHEMGLNVDE
                     IAARVHALSESNPMLGHRGCRLGNTYPEITETQTRAIIEAALNLKKKGIVAIPEI"
BASE COUNT          955 a          481 c          604 g          867 t
ORIGIN      
        1 gtgtgtttgg cgaaagtcaa gcacaagaat tggtccccaa agctcaatta ttacccaaag
       61 atattgagtg gcattttatt ggacatttac aaacgaataa agttaagtac attattcctt
      121 atgtaagtct tattcatgct gtagattcgt taaaattagt gcaagaaatt aataaacaag
      181 ctttacagca acatcgcata atggattgtt tagtgcaaat tcggattgct caagaagaaa
      241 cgaaatatgg aatgacattg gaagaatctg ccagtatgtt gcactatatt tcagtaaaca
      301 attttactgc tattcgtatt tgtggcgtga tgggattagc cactgataca gaagatcaga
      361 atcaagtaac aaaagaattt caatacctca catactgttt tcatacactt aaaaatactt
      421 tttttcaaac aagtttgtac ttttctgaaa tatccatggg tatgacacat gattatcctc
      481 ttgcgattga acaaggctct accttgatac gtgttggtag tggtattttt ggagaaagag
      541 tctactaatt tgcttatttt atttcttctt tttagctaaa taattgctaa acaaaacgct
      601 aaaattttta ttataatcag aaaaatttta tctttgcact ctatttgata aaatatacgt
      661 gtttattcaa aataattttt taatttatta attttatgga caagaaaaga gtttattttt
      721 ttggaaacaa aaccgctgaa ggtaacggct ctatgaaaga gttacttggt ggaaaagggg
      781 ctaacttggc agatatgaac ctgattggtg tgcctgttcc tccgggaatg acaattacaa
      841 cagaggtgtg taatgagtat tttttaatcg gtcaagaaga ggttattaaa accattaagc
      901 cagaagttga aaaagcaatg aaacaaattg aagatgctgt tgctggtcca aaatttggcg
      961 ataacaaaaa tcctttattg gtatctgtac gttctggagc tagagcttct atgccaggta
     1021 tgatggatac tattttgaac ttagggttaa atgacgaagc tgttgaaact atcgctcaaa
     1081 aaagtggtaa tccacgtttc gcatgggatt cttaccgtcg ttttgtacaa atgtatggcg
     1141 acgtggtact aggcatgaaa ccagaatcaa aacaagatga agacccattt gaagtaatca
     1201 tcgaaaagat gaaacatgaa aaaaaggtag aattagatac tcaattaaca accgaagatt
     1261 taaaagaatt ggttgctaaa ttcaaaaaag ctgtaaaaga cgttacaggt aaagattttc
     1321 cagtttctcc atgggaacaa ctttggggtg ctgttatggc agtattcaat agctggatga
     1381 acgaaagagc tattctgtat cgcaaaatga ataaaattcc tgctgaatgg ggaacagctg
     1441 ttaacgtaca agcaatggta tttggtaaca tgggtaacaa ttctgctaca ggtgtggcat
     1501 ttactcgtga tgctagtaca ggtgaagata ttttcaatgg cgaatattta attaatgctc
     1561 aaggtgaaga cgtggtagcg ggtattcgta caccacaaga aattaccatc gaaggttctc
     1621 gtcgttgggc taaaatgcaa ggcatcagtg aagctgatcg tctagctaaa tacccttcat
     1681 tagaagaaac aatgccttca gcttatgctg acttaaacgc tattcaacaa aaattagaag
     1741 accactatac agatatgcaa gatatcgaat ttactatcca agatggtaaa ttgtggatgc
     1801 ttcaaacacg taacgctaaa cgtactggtg ctgctatggt aagagtagct atggaaatgg
     1861 ttaaacaagg cattatcgac gaaaaaactg cattattgcg ttgcgaacca gaaaaattag
     1921 acgaactttt acatccagta tttgatacaa aagcattagc acaagcaaaa gccttatctc
     1981 aaggtttacc tgcttctcca ggtgctgcta ctggtcaagt agtattcttt gcagaagatg
     2041 cagaagcatg ggctgctgaa ggtaaaaaaa ctatcctttg ccgtgtagaa acatctccag
     2101 aagaccttaa aggtatgtat gctgctgaag gtattcttac tgctcgtggt ggtatgacat
     2161 cgcacgctgc tgtagtagct cgtggtatgg gtaaatgctg tatctctgct gctaatggtt
     2221 tggttattga ttacaaaact cgtacaatga cagtaggtgg agctaccatt aaagaaggtg
     2281 agtgggtatc attaaatggt actactggta ttctttatgc tggtaaagtg gaaaccaaag
     2341 cggctgaatt aagtggtgac tttgctgaat taatgaaaat agctgacaaa tatgcacgtt
     2401 tacaagtacg tacgaatgct gatacgcctc acgatgctca agtagctcgt aacttcggtg
     2461 ctcaaggtat tggtcttact cgtactgaac acatgttctt cgaaggtgat aaaattaaag
     2521 ccatgcgcga aatgattctt tctgaagatg aagctggtcg tcgtaaagct ttagtaaaat
     2581 tattaccatt acaaagagaa gactttgaag gtatcttcga agcaatgaac ggacttccag
     2641 taacggttcg tttacttgac ccacctttgc atgagtttgt tcctcacgat ccaaaaggtc
     2701 aattggaaat ggctcacgaa atgggcttaa atgttgacga aattgctgct cgtgtacacg
     2761 ctctttctga gtcaaaccca atgttaggac accgtggttg tcgtttaggt aacacatatc
     2821 cagaaattac tgaaacacaa actcgtgcta tcatcgaggc tgctcttaac ttgaaaaaga
     2881 aaggcattgt agctattcca gaaatca
//
LOCUS       DAOPUR010000173         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_35427_length_2907_cov_2.411992, whole genome shotgun sequence.
ACCESSION   DAOPUR010000173 DAOPUR010000000
VERSION     DAOPUR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35427_length_2907_cov_2.411992"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKN90_03875"
     CDS             <1..689
                     /locus_tag="PKN90_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNZ85450.1"
                     /translation="FSDDFLRENLAFRGGTALHKLYLNPAPRYSEDIDLVQIKKGPIK
                     PIMERLNEVINFFEEKRATQVRGHGAKALYRFTSEYEEIRMRLKLEINCKEHFNVLDW
                     VDFPFEIKSEWFTGKAEIRTYNINELLGTKLRALYQRSKGRDLFDLDYARQNMDLDYE
                     QIITCFKEYTSYATGKKPPSKKEFLLNIEEKELSAEFIGDMEALLRPEIKYNQRDAFN
                     WLKTELIETI"
     gene            882..1298
                     /locus_tag="PKN90_03880"
     CDS             882..1298
                     /locus_tag="PKN90_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85451.1"
                     /translation="MNKAQAQTHVIRFKRYSRKAYAAFCSMHKHITIGKLATALCDKQ
                     LAKSKLAHTSSADCLAFYTPVFSDESIVDNEMFAFDVLPLGCVAVIPTTYSTTEGQEA
                     RISCSDCLYTLLLYLMVFSTAFLFAHTLYLLNPFMI"
     gene            1295..>2907
                     /locus_tag="PKN90_03885"
     CDS             1295..>2907
                     /locus_tag="PKN90_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ85452.1"
                     /translation="MNKKKILSLTLLASLLPQLGMMAQSNSSNTQQLTEISVHAQPTK
                     VYSDHLRVVMSIDKSTIAQMPVQTVQELLNNLPGLDVRQRGGSGVQADISMRGGTFDQ
                     VLILLNGVAISDPQTGHHTLNLPIDLNMVERVEILQGGAISLFGTNALSGAINIVTNE
                     KTDDALKVSLTGGSFGLFNPSVASTFASNKWRWAISASHNESAGYTENTDYRYTQGFV
                     QAQFQATEQDTWKWQIGVQDKAFGANSFYSVNFPNQFEKTKTLISSISWDKRINSLLW
                     QSTAYYRAHFDQFELFRNYENAASWYTFHNYHLSHVSGINSRLSWFTSLGKSSIGIEG
                     RNEHINSNVLGELKTTPQQIPFAKNTNTQFLYEKNRTNVQYFAEQTIYLQAFTASVGV
                     SGNYNTQFGNRYSYGLNTSYAFNTYSQVFANINKSLRLPTFTDLYYKSATQESNPNLK
                     PEEAITAEIGAKYTKQAWQWNANAFYRIANNSIDWVRSLETEKWKSMNHTRIDAMGGE
                     IAAAYTGGFWAKNIQGSYAYVHLNKDAGD"
BASE COUNT          958 a          556 c          585 g          808 t
ORIGIN      
        1 tattctctga tgacttcttg agagaaaatc ttgcattcag aggaggaaca gctttgcaca
       61 agctatatct taatcctgca cctcgatatt ctgaggatat tgatttggta caaattaaaa
      121 aaggtccaat aaaacccata atggaaaggt taaatgaggt tataaacttt ttcgaagaga
      181 aaagagctac tcaagtacgt ggacatggag ccaaagcgtt ataccgtttt acttcggaat
      241 atgaagaaat acgaatgcga ttgaaattgg aaataaactg taaagagcat ttcaatgtat
      301 tggattgggt cgattttcct tttgaaatta aaagtgaatg gtttacaggt aaagcagaaa
      361 taagaacgta caatataaat gaacttttag ggacaaaact cagggcttta tatcaacgaa
      421 gcaaaggaag agatttattt gatttagatt atgcacgaca aaatatggat ttagactatg
      481 agcaaatcat aacttgtttt aaggaataca cttcgtatgc cacaggtaaa aaacctccta
      541 gtaaaaaaga attcttactt aacatagaag aaaaagagct tagtgctgag tttataggtg
      601 atatggaagc attattaaga cccgaaatta aatacaatca gcgtgatgct tttaattggt
      661 tgaaaacgga attaattgaa acaatttaaa tgagaaaata atgttgctct attcctttaa
      721 atacacccag tcaggacgga cttctcaatt aagagagttc ggaagacttt tctgagaacc
      781 aacgacttgg ggcagtgcaa gaacacataa agtaaaaaat ctgatgacaa agctcgtcat
      841 ttaatttttt gcattacctt tgtagcgaaa atttacattc catgaacaaa gcacaagcac
      901 aaacacatgt tattcgtttt aagcgatact cgcgtaaggc gtatgctgct ttttgttcga
      961 tgcacaagca cattaccatt ggtaaacttg ccacagcact ttgcgacaaa caattggcta
     1021 aaagcaaact agcacataca agttcggctg attgtttggc tttttataca ccagtttttt
     1081 ctgatgaatc aattgtggac aatgagatgt ttgcttttga tgtgttgccg ttggggtgtg
     1141 ttgctgttat ccctaccact tattctacta ctgagggtca ggaagctcgt atttcctgct
     1201 cggattgttt atacacgctg ttgttgtacc tgatggtatt ttcaacagcg tttttgtttg
     1261 cacatacttt gtatttactt aaccctttta tgatatgaac aagaaaaaaa ttctttcctt
     1321 gacattgctt gcctccctat tgcctcaact tggaatgatg gcacaaagca atagttctaa
     1381 tacgcaacag ctaactgaaa tcagtgttca cgctcagccc accaaggtgt attcagacca
     1441 tctgcgagta gtgatgagta tagacaagag cacaatagct caaatgcctg tacaaactgt
     1501 tcaagagcta ctcaacaacc tgcccggact ggacgttcgc cagcgtggtg gtagtggtgt
     1561 gcaagccgat atcagtatgc gaggaggcac tttcgaccaa gtgttaatct tactcaatgg
     1621 cgtagctatc agcgacccac aaacgggtca tcatacgctt aacctgccta tcgatttaaa
     1681 tatggtagag cgagtagaaa tattgcaagg cggagccatc agtctctttg gaaccaatgc
     1741 cctaagtggt gctatcaata ttgttaccaa cgaaaaaaca gacgatgccc tgaaagtgag
     1801 cctcactggt ggttcgtttg gcttatttaa cccctcagtg gcaagtacat ttgcgagcaa
     1861 caaatggcga tgggcaatat ccgcttcgca caacgagtca gcgggctata ccgagaatac
     1921 cgactatagg tatacacaag gttttgttca agcacaattt caagctacgg aacaagacac
     1981 ttggaaatgg caaataggcg tgcaagacaa agcttttgga gcaaattcat tttattcggt
     2041 aaattttccg aatcaatttg aaaaaacaaa aacccttatc agcagtatca gttgggataa
     2101 acgcattaat tccctattgt ggcaatcaac tgcctattac agagcacatt tcgatcagtt
     2161 tgagcttttt cgcaattatg aaaatgcggc atcttggtac acgtttcaca attatcacct
     2221 cagtcatgta agtggcatta actcaaggct gtcgtggttt actagtttag gaaaatcaag
     2281 catcggcata gagggtcgca acgaacatat taacagcaat gtgttgggag aactaaaaac
     2341 aacccctcaa caaattccgt ttgccaaaaa taccaatacg cagtttttat acgaaaaaaa
     2401 ccgtaccaat gtgcaatatt ttgccgaaca aacaatttac ttacaggcat ttacggcttc
     2461 tgttggtgtg tcgggcaact ataacacgca atttggcaac aggtatagtt acgggcttaa
     2521 tacttcgtat gcattcaata cgtattctca ggtatttgca aatatcaata aatcgttacg
     2581 actacctacc tttactgatt tatactacaa aagtgctacg caagagagta acccaaactt
     2641 aaaacctgaa gaagctatca ccgcagaaat aggagccaaa tacaccaaac aggcatggca
     2701 atggaatgca aacgcatttt accgtatagc caacaatagt atcgactggg tacgttcgct
     2761 cgaaacagaa aaatggaaaa gcatgaacca tactcgcatt gatgctatgg gaggagaaat
     2821 agcggcagct tatacaggtg gtttttgggc aaaaaacata caaggatcgt atgcctacgt
     2881 tcatctgaac aaagatgcgg gcgatat
//
LOCUS       DAOPUR010000174         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36065_length_2871_cov_2.777344, whole genome shotgun sequence.
ACCESSION   DAOPUR010000174 DAOPUR010000000
VERSION     DAOPUR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36065_length_2871_cov_2.777344"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKN90_03890"
     CDS             <1..779
                     /locus_tag="PKN90_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85453.1"
                     /translation="FMPSVGIELITFRDLATSPLFYDGVGLAGSIAWQWEHPKNETKF
                     ALYATSSTTTSRTPLSAFFQTSTSSSFLSGSVYVHSLFTLPSLSNDKRTVQLGGAMAN
                     TWNIRSNPSLFNNGFGMESFANLFLSGKGIYDISRKHSVVQDFVLFTRTLQPAKRNIS
                     LQLNIGLLNLNYRPGYAYNADVALDGSNTDPLAFLLDGHVWKINGYRLGTQLDYTKYI
                     PNGNGHRWSYIWDIAQAPGRFEPFEMVTHRIQYTLLIQTK"
     gene            789..1787
                     /locus_tag="PKN90_03895"
     CDS             789..1787
                     /locus_tag="PKN90_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ85454.1"
                     /translation="MRTTLLCLLIALTLTSCEDYFFEPDKASSNPYDNFEYLWNELDK
                     KYSYFELKNIDWDEMKTTYEARLYPNMSETELFNTLADLMNELRDDHSNLVSPFNVSV
                     YNVPLTKPANYHARTIDEFYIPHAERTGSFKHAMLSGTNIAYIRYASFSDDISNTHLD
                     YLLDKYRYTDGIILDLRDNGGGSMFNIPMLLERFIDSRTLVAYTLTRNGKGRNDFSDR
                     ATFYMNPYKGTRYTKPIMVLIDRGSYSATTFFALSTKAIPNLTLVGDTTGGGGGMPNG
                     GQLPNGWTYRFSISQLLDLDGNNYAEDGVPPDILVSFDWTDLTKDEIIERAIQEID"
     gene            1849..2835
                     /locus_tag="PKN90_03900"
     CDS             1849..2835
                     /locus_tag="PKN90_03900"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HNZ85455.1"
                     /translation="MKIACFSTKSYDKEYLSKAIVAPHSIVFFEESLNPNTVGLLTGF
                     DGICVFVNDKLDNNLLQQIASAGIKAIFLRCAGFNNVVISTATSLGIKVYRVPAYSPY
                     AVAEHAVALVLTLNRKTHKAYNRVKEYNFSIERLHGFDVHGKTVGVIGTGTIGLVFAK
                     IMLGFGCKVIAFDPTESDDAKQLGIQYVALEDVLKQADILSLHCPLNQHTRHMISTKE
                     FSIMKHGAMLINTSRGALIDTKEAIEALKTGALGYLGIDVYEEEEKLFFHDFSESIIS
                     DDILMRLMTFPNVLITSHQAFFTHEALTQIAETTYQNMLDFTHGTSSTNQLL"
BASE COUNT          932 a          585 c          518 g          836 t
ORIGIN      
        1 attttatgcc ctcagtaggc attgagctaa taacttttcg cgatttggct acttcgccct
       61 tattttatga tggtgtgggt ttggctggaa gcatagcttg gcaatgggaa catcccaaaa
      121 atgaaacaaa atttgcctta tatgccacgt ctagtactac aacttcacga acaccattaa
      181 gtgcattttt tcaaacaagc acatcttctt cttttctaag tggcagtgta tatgtgcact
      241 ccctctttac actaccctct ctttccaacg acaaacgaac agtgcaacta ggtggagcta
      301 tggctaacac atggaatatt cgtagcaatc cctctttatt caacaatggg tttggcatgg
      361 aaagttttgc caatctgttc ctttcaggaa aaggcatata tgatatttct agaaaacata
      421 gtgtagtaca agattttgta ttattcactc gtacacttca acccgcaaaa cgaaacatat
      481 cattacaact taatatagga ttgctcaatc ttaactacag acccggatac gcatacaacg
      541 ctgacgtagc tttagatggg tctaatacag acccattagc ctttttgctt gatggacatg
      601 tatggaaaat caatggttat aggctgggaa ctcaacttga ctacaccaaa tacatcccca
      661 atggcaacgg gcatcgctgg tcgtacatat gggatatagc acaggctcct ggacgtttcg
      721 agccttttga aatggtaacg caccgcattc aatatacatt acttatacaa actaaataac
      781 atactgtcat gagaacaaca cttttatgtc ttttgatagc acttactttg acatcgtgcg
      841 aagattattt ttttgaaccc gacaaagcct cttccaaccc atacgataat tttgaatatc
      901 tatggaatga actagacaaa aaatacagct attttgaact aaagaatatc gattgggacg
      961 aaatgaaaac aacctacgaa gctcgccttt acccaaacat gagcgaaaca gagctgttca
     1021 ataccctagc cgatttaatg aacgaactac gtgatgacca tagtaacttg gtatcgccat
     1081 tcaatgtatc tgtgtataac gtgcctctta caaaacctgc taactatcat gcacgtacaa
     1141 tagatgaatt ttacatacca catgctgaac gaacaggttc atttaagcat gcaatgctct
     1201 caggtaccaa tattgcatac attcgttatg caagtttctc tgatgatatt tctaatactc
     1261 atcttgatta tttattagat aaataccgct atactgatgg tatcatattg gacttacgcg
     1321 acaatggagg aggtagcatg tttaacattc ctatgttatt agaacgcttt atcgactcac
     1381 gcaccttggt cgcttataca cttacccgta acggaaaagg aagaaatgat ttttcggaca
     1441 gagctacttt ttatatgaac ccatacaagg gaacacgata tactaaaccc ataatggtac
     1501 ttattgaccg tggctcgtat agtgcaacaa cattttttgc attatcaacc aaggcaatcc
     1561 caaatttaac ccttgtaggc gatactactg gtggcggtgg cggtatgcct aatggcggtc
     1621 aattacccaa tggatggacc tatcgattta gcatatctca actacttgac cttgatggga
     1681 acaattacgc cgaagatggt gttccgccag atattttggt aagtttcgat tggacagatt
     1741 tgaccaaaga cgaaattatt gaacgtgcga tacaagaaat agactaaaaa acttgctatt
     1801 ttcagtattt atgagtagtt ttgaagagta ctctaaattg tcaatcatat gaaaatagca
     1861 tgctttagta caaaatcgta cgacaaagaa tatttatcaa aagctatagt tgcaccacat
     1921 agcatcgtat ttttcgaaga atcgctcaac ccaaacaccg ttggtttgct tactggtttt
     1981 gatggcattt gcgtatttgt aaacgacaaa ttagataaca accttttaca acaaatagca
     2041 agtgctggta ttaaagccat ctttttgcgt tgtgcaggtt ttaataacgt agtcataagc
     2101 actgcaacat cattgggaat aaaagtatat cgtgtacccg cctattctcc ctatgcagta
     2161 gcagaacacg cagtagcact ggttcttacc ctaaacagaa aaacacataa agcatacaac
     2221 cgcgtaaaag aatataattt ttcgatagaa cgcctacacg gatttgatgt gcatggcaaa
     2281 acagtaggtg taattggcac agggactatt ggattggttt ttgccaaaat aatgcttggt
     2341 tttgggtgca aggtaatcgc attcgaccca acagaatctg acgatgccaa acaactagga
     2401 atccagtatg tagccttaga agatgtacta aaacaagctg atatcctttc gttacattgc
     2461 ccactcaatc agcatacccg acacatgatt agtaccaaag aatttagcat aatgaagcac
     2521 ggggcaatgt taataaacac cagcagaggg gcacttatcg ataccaaaga agcaatagaa
     2581 gctcttaaaa caggtgcatt gggttacttg gggattgatg tatatgagga agaagaaaaa
     2641 cttttctttc acgatttttc ggaaagcatt atttctgatg atattttaat gcgattaatg
     2701 acttttccaa acgtactgat tacgtctcat caagctttct ttacacacga agcacttaca
     2761 caaattgccg aaacaaccta tcaaaatatg ctcgatttta cgcacggtac ttcatcaact
     2821 aatcaattac tataaccatg atgactaaca gtagtaacat cgaacttaaa a
//
LOCUS       DAOPUR010000175         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36080_length_2870_cov_3.016696, whole genome shotgun sequence.
ACCESSION   DAOPUR010000175 DAOPUR010000000
VERSION     DAOPUR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36080_length_2870_cov_3.016696"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..651)
                     /locus_tag="PKN90_03905"
     CDS             complement(124..651)
                     /locus_tag="PKN90_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNZ85456.1"
                     /translation="MKKTRFTSIGYLIIPLLIAVMGVSCSVSYKFSGTSIDYSRTKTI
                     YVRDFTNQAQMVYAPLTDVLNETVKDIYARQTRLSLVNRNGDLEIEGEITNYDISSMS
                     IQADAIAAETRLTIRVKVRFTNNKNHDEDFERTYTSFRNFSSNLMLNDVQDELITEMT
                     KEIAELIFNDTVANW"
     gene            complement(675..1895)
                     /locus_tag="PKN90_03910"
     CDS             complement(675..1895)
                     /locus_tag="PKN90_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ85457.1"
                     /translation="MDEKELQAIKQRFQIIGNTPALNRAIEIATQVAGTDLSVLITGE
                     SGVGKESFPKIIHQFSPRKHEKYIAVNCGAIPEGTIDSELFGHEKGSFTGALNDRKGY
                     FEVADGGTIFLDEVGELPLPTQARLLRVLEVGEFIKVGSSNVLKTNVRVVAATNLNIP
                     KAIQEGKFREDLYYRLNAVQIAVPPLRERKNDIPLLFKKFASDFAEKYRMPTIYLTPE
                     AQEVLVDYYWQGNIRQLKNIVEQLSIIEEHREITSDCLQNYLPKPTKEQLPLLIANTH
                     NNEKSFSNEREILYQVLFDMKKDIDELKKMVHQYKQPPVTPTKNTDNEPSFLPSIVDK
                     NTLPKTEKHIKEVEIEDAQEYEEHNFSVNEMEKNLIKKALEKHNGKRKLAALDLKISE
                     RTLYRKIKEYDLDA"
     gene            complement(1932..>2870)
                     /gene="pdxA"
                     /locus_tag="PKN90_03915"
     CDS             complement(1932..>2870)
                     /gene="pdxA"
                     /locus_tag="PKN90_03915"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNZ85458.1"
                     /translation="ILKTLDDARILEFCTPILYGSPKVAAYHRKNLEITLNLNLITNA
                     AEANEKRVNIINCNDDEIKVELARSTDEAGKAAYLALEKATQDLKSGAIDALVTAPIN
                     KKNIQSDEFTFPGHTEYLEEKFGNKGEALMLMVNDFMRIAVVTGHIPITKVAQAITTE
                     KIVEKIHQLNDALKQDFMLTKPRIAVLGLNPHAGDDGVIGDEEKTIILPAIEKALQEG
                     IICVGPLPADGFFGASNFHHYDGILAMYHDQGLIPFKALSMDSGVNYTAGLSVVRTSP
                     AHGTAYDKAGQNLANESSFREALYLACDIVNNRQRS"
BASE COUNT          752 a          522 c          566 g         1030 t
ORIGIN      
        1 agttggtgta tgccatgaaa aaagggatat ttattggcaa tctctttcat gtcagcaaga
       61 gtatggtttc cgagtttatc gggatgttcc atataaaaca gtatatcttg tccagtcata
      121 gttctaccaa tttgctacag tatcgttaaa aatcagttct gctatttctt ttgtcatttc
      181 agtaattaat tcatcttgca catcatttag catcaggtta ctactaaaat tacggaaaga
      241 tgtgtacgtg cgttcaaaat cttcgtcgtg gtttttattg ttcgtaaaac gtacttttac
      301 gcgtatggtt agtcgtgttt cggcagcaat agcatctgcc tgaatagaca tagacgaaat
      361 gtcgtagttt gtaatttctc cctctatttc taagtcccca tttcggttga ctagcgaaag
      421 gcgtgtttgg cgagcatata tatcttttac tgtttcgttt aatacatcag taagaggagc
      481 atataccatt tgagcctgat tggtaaaatc gcgtacgtaa attgttttgg tacgactata
      541 atcaatagaa gtgcctgaga atttgtatga tacagagcaa gaaacgccca ttactgctat
      601 tagtaatgga ataattaggt agccaatgga tgtaaaacgt gtttttttca tggtttgtgt
      661 gcgttaaaat ctgactaagc gtctaaatca tattctttaa ttttacgata taaggtgcgt
      721 tcagatattt ttaaatccaa ggcagccaat ttgcgtttac cattgtgttt ttcgagtgct
      781 ttttttatca agtttttctc catttcgttt acactaaaat tgtgttcttc gtactcttga
      841 gcatcttcga tttctacttc tttaatgtgc ttttctgttt ttggcaacgt atttttatcc
      901 acaattgatg gtaaaaaaga aggttcgtta tccgtatttt ttgtcggagt tacagggggt
      961 tgtttgtact gatgcaccat tttttttagt tcatcaatgt cctttttcat atcgaataac
     1021 acttggtata agatttctcg ttcgttagaa aacgattttt cgttgttatg tgtgtttgct
     1081 ataagcaacg gtaactgttc tttcgtgggt tttggtaaat agttttgcaa gcaatcgctt
     1141 gtaatttctc tgtgctcttc aataatagat agttgctcaa cgatgttttt taattgacga
     1201 atattccctt gccaataata atcaaccaat acttcttgag cctcaggggt aaggtaaata
     1261 gtaggcatac ggtatttttc ggcaaagtcg gatgcaaatt ttttaaataa taaggggata
     1321 tcattttttc tttcgcgtag tgggggtact gctatttgta cagcattgag tctgtaatac
     1381 aaatcttcac gaaatttacc ttcttgtatg gctttgggta tgttcaaatt agtggcagct
     1441 actacacgta cgtttgtttt taatacattc gatgagccca ccttgataaa ttcgcccact
     1501 tcgagcacac gcaacaaacg agcttgggta ggcaaaggta gttcgcccac ttcgtctaaa
     1561 aagattgtgc caccatcggc tacttcaaaa taaccttttc ggtcgttgag tgctcctgta
     1621 aaagagcctt tttcgtgtcc aaaaagttcc gaatcaatcg ttccttcggg tatagcacca
     1681 cagtttacgg caatgtattt ttcgtgttta cgagggctaa attggtgtat aattttcgga
     1741 aaactttctt ttcctacacc gctttcgccc gttattagta cagataagtc tgtgccagct
     1801 acttgagtag ctatttcaat agccctattg agtgctggcg tattgccaat aatctgaaaa
     1861 cgttgtttta ttgcttgtaa ttctttttcg tcaatcatac tacaatcctt tccactaaag
     1921 tgttgaatag attagcttcg ttgtctgttg ttaactatat cacatgccaa atataatgcc
     1981 tctctaaaag acgattcgtt ggcaagattt tgccctgctt tgtcgtaggc agtgccatga
     2041 gcaggcgagg tgcgtactac ggataatcct gctgtataat ttacgcccga atccattgat
     2101 aaggctttga aagggattaa accttggtcg tgatacatcg ccaaaatgcc atcgtaatga
     2161 tgaaaatttg atgcaccaaa gaacccatca gcgggtagtg gtcctacgca aataattcct
     2221 tcttgcagtg ctttttcaat agctggcaaa atgattgttt tttcttcatc tcctattacg
     2281 ccatcatcac cagcatgcgg atttaagcct aaaacagcta tacgtggttt ggttaacata
     2341 aaatcttgtt tcagggcatc gttgagttgg tgaatctttt ctacaatttt ttcggttgta
     2401 atggcttgag ctactttggt aatgggaata tgacctgtaa caacagcaat acgcataaaa
     2461 tcattcacca tcaacatgag tgcttcgcct ttgttgccaa atttttcttc taaatattcg
     2521 gtatgaccgg gaaaagtaaa ttcatctgat tgtatattct ttttattgat aggagctgta
     2581 actagggcat ctattgcacc tgatttgagg tcttgagttg ctttttccag agctaagtaa
     2641 gcagctttgc cagcctcatc cgtagagcgt gctagttcta cttttatttc atcatcgttg
     2701 cagttgataa tgtttactcg tttctcattt gcctcagctg cattggtaat aaggttgagg
     2761 ttaagcgtta tttctaagtt tttgcggtga taggctgcta ctttgggcga accgtatagt
     2821 ataggtgtgc aaaattcaag aatgcgggca tcatctaatg ttttcagaat
//
LOCUS       DAOPUR010000176         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36137_length_2867_cov_3.264936, whole genome shotgun sequence.
ACCESSION   DAOPUR010000176 DAOPUR010000000
VERSION     DAOPUR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36137_length_2867_cov_3.264936"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PKN90_03920"
     CDS             complement(<1..632)
                     /locus_tag="PKN90_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ85459.1"
                     /translation="MTKIIVTGGTGYIGSHTVVELQQAGYEVVIIDNLSNSSVDVVQG
                     IFAITGVMPSFEKIDCTDYQAMESVFKKHADAQAIIHFAASKAVNESVEKPLLYYRNN
                     LVSLMNLLELMATYTMPNLVFSSSCTVYGQPDILPVTENSPIKVSLSPYGNTKQIGEE
                     IITDTVHANKSIHAIILRYFNPVGAHESALIGELPNGVPQNLMPFVTQTA"
     gene            780..1205
                     /gene="ruvX"
                     /locus_tag="PKN90_03925"
     CDS             780..1205
                     /gene="ruvX"
                     /locus_tag="PKN90_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNZ85460.1"
                     /translation="MGRIIAIDYGKKRVGIAVTDTLQLIANGLTTVASHEVIAFLERY
                     IQTEPVDKIIIGLPKQMNNELSESMKYITPFVHTLKKRITHIPIEYVDERFTSVLAHR
                     TMLEAGLKKKDRQNKALVDTISATIILQTYLESIKTNTL"
     gene            1202..1753
                     /gene="def"
                     /locus_tag="PKN90_03930"
     CDS             1202..1753
                     /gene="def"
                     /locus_tag="PKN90_03930"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNZ85461.1"
                     /translation="MIRPIVVYGNAVLRKKADPIDKNYPELSSLINDMFDTLSHAEGV
                     GLAAPQVGLPIRLFIVDLSILGDDEPSFVNFKKVMINPLITSFEGEELSQEEGCLSIP
                     GISETVKRSDKISIEYYDENWQKHVEQYSGFPSRVMQHEYDHIEGHVFVDKISPIRRQ
                     MISSKLNALTKGKFNCHYKVQKV"
     gene            complement(1750..2331)
                     /gene="coaE"
                     /locus_tag="PKN90_03935"
     CDS             complement(1750..2331)
                     /gene="coaE"
                     /locus_tag="PKN90_03935"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNZ85462.1"
                     /translation="MLKVGITGGIGSGKTHVCQLLETVGIPIFYSDIVGKEITNTNPK
                     VRAAIIELLGEEAYKNDVLNAKFVASKVFDDKSLLEKINAIIHPVVFAAFEDWSMQFE
                     GHKIVALESAVLFESGFDKYIDKSIVVTAPLQVRINRIMKRDKLTYPEVEARIKNQLS
                     DEEREARADFVIKNDGVEHLPSQLFAFLKAVDF"
     gene            complement(2338..>2867)
                     /locus_tag="PKN90_03940"
     CDS             complement(2338..>2867)
                     /locus_tag="PKN90_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HNZ85463.1"
                     /translation="VRLRYDFSYRKQYSLAGDLIVSPNEVTIYGNKERIDTIHYVTTE
                     RVVLTDLTDTTKITVLVEKLPHTRFLPNNVSVFIPVEVFTESSMHIPITVHNVPDNVA
                     VRVIPADVEVLYQVGKSMYNKVNPMDFVISVDYNNVVRSTSRRQILTLEKYPTYLRNV
                     KLGTMEIDCFIEIKD"
BASE COUNT          961 a          550 c          457 g          899 t
ORIGIN      
        1 atagctgttt gcgttacaaa aggcattaaa ttttgtggca caccgttggg caattctcca
       61 atcaaggcac tttcgtgtgc tcctactgga ttaaaataac gtaaaataat ggcatgaatg
      121 cttttgtttg catgtactgt atcggtaata atctcctcac caatttgttt ggtattacca
      181 taaggagaaa gggatacttt tattggagaa ttttcagtta caggcaatat atctggttga
      241 ccatatacgg tgcacgatga cgaaaacacc aaatttggca tagtatatgt agccattaat
      301 tctaatagat tcatcaacga cactaaatta ttgcggtagt acaacaaagg cttttccacc
      361 gattcattca ccgccttact ggcagcaaaa tgaataatcg cttgtgcatc tgcgtgtttt
      421 ttgaatacag actccattgc ttgataatca gtacaatcaa tcttttcaaa acttggcata
      481 acgcccgtga tagcaaaaat accttgcacc acatctacag aagaattaga taagttgtca
      541 atgatgacaa cttcataacc agcttgttgt aactcaacca ctgtatgcga acctatataa
      601 cccgtacctc ctgtaacaat aatttttgtc atacaattaa atttttgcac aaagatacat
      661 ctttttcaga tacagagtag aaagtgccca tgctaagggt taaaaaagtg tctaaatttt
      721 taaaaaatac tagtaaattt gcttttatgg cggtttatag ctacttttgg tgcttaatta
      781 tgggaagaat tattgctatt gattatggca aaaaacgtgt agggatagct gttaccgaca
      841 ctttgcaact gattgccaac ggattaacaa cggtggcaag tcacgaagtt attgcttttt
      901 tagaacgcta tatacaaaca gaacccgtgg ataaaatcat tataggatta cccaaacaaa
      961 tgaataatga gctatctgaa tcgatgaaat acatcacgcc gtttgtacat acgctcaaaa
     1021 agagaatcac gcatattccc attgaatacg tagatgagcg atttacatct gtgttagctc
     1081 atcgtaccat gctcgaggct ggattaaaaa agaaagatag acaaaacaaa gctttggtgg
     1141 ataccattag tgcaaccatc attttacaaa cataccttga aagtataaaa actaacacat
     1201 tatgatacgt cctatagttg tttatggcaa tgctgtatta cggaaaaaag ctgacccaat
     1261 tgacaaaaac tacccagaac tcagttcatt aatcaacgac atgtttgata ccctttctca
     1321 tgcagaggga gtaggattag ccgcaccaca agtgggactc cctattcgtt tatttattgt
     1381 tgatttgtca attttaggtg atgacgaacc aagttttgta aatttcaaga aagtaatgat
     1441 taatccttta attacatctt ttgagggaga agaattatct caagaagaag gatgcttaag
     1501 catacctggt atttctgaaa cagtaaaaag aagcgacaaa atttcaattg aatactatga
     1561 cgaaaattgg caaaaacacg ttgagcaata cagtggtttc cctagcagag taatgcaaca
     1621 cgaatacgac catatcgaag ggcatgtatt tgtagataag atatcaccta tacgcaggca
     1681 aatgatttcg tcaaaactga acgcattaac gaaaggtaaa tttaattgcc attacaaagt
     1741 gcagaaagtt taaaaatcaa ctgccttcaa aaaagcaaat aattgagaag gcaagtgctc
     1801 caccccatcg tttttaataa caaaatcggc tcttgcttct ctttcttcat cagaaagttg
     1861 atttttaatt cttgcttcta cctctggata agtaagttta tctcttttca taattctatt
     1921 aatgcgtacc tgtaatggtg ctgttaccac aatagactta tcaatatact tgtcaaaacc
     1981 actttcaaac aatacagcag attctaatgc gactattttg tgcccttcaa actgcatgct
     2041 ccaatcttca aaagctgcaa atactacggg atgaataatt gcatttattt tctctaacaa
     2101 agatttatcg tcaaacactt tactcgcaac aaatttagca ttcaacacat cattcttata
     2161 tgcctcttca cccaataact ctataatcgc agcacgaact ttaggatttg tattggttat
     2221 ttctttgcca acaatatcag aataaaaaat tggtatacca actgtttcta gtaattgaca
     2281 tacatgtgtt ttaccactac caattcctcc tgttatacct actttcaaca tacacaatta
     2341 atcttttatt tcaataaaac aatcaatttc catagtacct aacttcacat ttcgtaggta
     2401 agttgggtat ttctctagcg taagaatttg tcttcttgac gtacttcgca ccacattatt
     2461 ataatccaca ctaataacaa agtccattgg atttacctta ttgtacatac ttttgcctac
     2521 ttgatacaga acctcaacat ctgcaggtat tactctaaca gcaacattat ctggtacatt
     2581 atgcactgta ataggaatat gcatcgatga ttctgtaaac acttccactg gaataaatac
     2641 agaaacatta ttgggcaaga aacgtgtgtg tggtaatttc tctaccaaaa cagtaatttt
     2701 tgtggtatca gttaggtccg tcaatactac cctttcagta gtaacatagt ggatagtatc
     2761 aattctttct ttgtttccat atatggttac ttcattagga gacacaatca aatctcctgc
     2821 aagactatat tgctttcggt aactaaaatc atatctcaat ctaacag
//
LOCUS       DAOPUR010000177         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36217_length_2863_cov_3.003561, whole genome shotgun sequence.
ACCESSION   DAOPUR010000177 DAOPUR010000000
VERSION     DAOPUR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36217_length_2863_cov_3.003561"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKN90_03945"
     CDS             complement(<1..205)
                     /locus_tag="PKN90_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ85464.1"
                     /translation="MKVYQTNEIKNISLVGSSGSGKTTLTEAMLFESGVIKRRGSIEA
                     KNTVSDYFPVEHEYGYSVFPTVFH"
     gene            complement(571..1671)
                     /gene="ychF"
                     /locus_tag="PKN90_03950"
     CDS             complement(571..1671)
                     /gene="ychF"
                     /locus_tag="PKN90_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNZ85465.1"
                     /translation="MALQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVI
                     TVPDERLNKLAELVKPQRIVPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     VLRCFDNENITHVDGSINPVRDKEIIDTELQLKDLETVESRIAKVQKQAQTGGDKTAK
                     AVYDIYMQYKEALLQGKSARTVQLDKEQAKLVKDLCLLTNKPVMYVCNVDEASAVKGN
                     AYVDAVREAVKDENAEILIVAAATEADIAELETYEERQMFLEEVGLSESGVARLIKSA
                     YKLLNLETYFTAGVQEVRAWTYEKGWKAPQCAGVIHTDFEKGFIRAEVIKYIDFTTLG
                     SEAACKEAGKMSVEGKEYVVQDGDIMHFRFNV"
     gene            1841..2734
                     /locus_tag="PKN90_03955"
     CDS             1841..2734
                     /locus_tag="PKN90_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85466.1"
                     /translation="MNKSTIIRFSALFIAIFVVVTFLFVYERKKNNEKEAELNQIIAE
                     LEISKQESLDEFENLALEYENYHFKPGNDSLLFLIENEKQKVNQLLEELRTVKATNAR
                     RIRELRNELGSVRSVLQVYVNRVDSLSNVNKKLVRENKKVQQQYEEVVQTAKELEERA
                     SQLDKKITLAAILEASNIRLNTLNDKGRETKKRSRIAAFEICFSLSKNQTTSTGLKSA
                     YLRISNPQGNVMKNNQSSVFAFEDKEIDFSCKKEIEYTGNNLSTCVYYNIADEELVAG
                     LYEVTIFIDGNLIGSESFYIK"
     gene            2741..>2863
                     /locus_tag="PKN90_03960"
     CDS             2741..>2863
                     /locus_tag="PKN90_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNZ85467.1"
                     /translation="MAGIYIHIPFCKQRCGYCDFYSHTNLQLKQRYVDTLCKEIE"
BASE COUNT          939 a          543 c          476 g          905 t
ORIGIN      
        1 aatgaaaaac agttggaaaa acagagtaac catactcatg ttcgacagga aaataatcag
       61 aaaccgtatt ttttgcttca atactgcctc tacgttttat aactccactc tcaaagagca
      121 ttgcttctgt gagcgtggtt tttcctgagc cagaagatcc cacaagtgag atattcttaa
      181 tttcatttgt ttgatacact ttcatacaat tattggtatt aaggttacta aattgttgta
      241 aacgaatccc tcgaaagcaa aaaaaatgcc ttgcaatgta ggaaatagtt agtaaaaaaa
      301 caatagtgaa aatatgttgt gtaacaatat ttgtaacaat tttgtaatat gaagccaaaa
      361 atctgttaat ttaaagactt agtaaaccaa tattttgtag ttaattgtat tttgcccgta
      421 tattcgcata tgattttttc atagtatttg attttaaggt tagcaagtag gtagtcaggc
      481 gagactacct tttgtttttt ataggggtaa taaaaaaagg gggaaacaat gtttccccct
      541 ttttgattct tttattaaac agatattttt ttacacatta aacctaaaat gcataatatc
      601 tccatcttgt acaacatatt cttttccctc aacgctcatt tttccagcct ctttgcaagc
      661 agcttctgat cccaaggttg taaaatcaat atacttaatt acttcggctc taataaaacc
      721 tttttcaaaa tcggtatgaa taacacctgc acattgtggt gctttccagc ccttttcgta
      781 ggtccaagcc ctcacttctt gcacaccagc tgtaaaataa gtttctaaat ttagcaattt
      841 gtaagccgat ttaattaatc gtgctacacc cgactccgat aatcccactt cttctaaaaa
      901 catctgacgc tcctcgtagg tttctaattc ggcaatatct gcttcggtag cagccgcaac
      961 aatcaatatt tcagcatttt catcttttac cgcctcacgc acggcatcta cgtacgcatt
     1021 gccctttacg gcagacgctt cgtcaacgtt gcacacgtac atcacaggtt tattggtaag
     1081 caaacataaa tcttttacca atttagcttg ttccttatct aactgtacag ttcgggcaga
     1141 tttgccttgc aacaacgcct ctttgtattg catataaata tcgtatacgg ctttagctgt
     1201 tttatcgcca ccagtttgtg cttgtttttg cacttttgca atacgcgatt ctactgtttc
     1261 taaatctttt agttgcaatt ctgtgtcaat aatttctttg tctctaacgg ggttaattga
     1321 cccatccacg tgggttatat tttcgttgtc gaaacaacgc aatacatgca atatagcgtc
     1381 tgtttcgcga atattggcta aaaatttatt gcccagccct tcgcctttgc ttgctccttt
     1441 taccaaacca gcgatatcta caatctccac tgtagttggc actatgcgtt gcggtttaac
     1501 caattcagct aatttattta gcctttcgtc gggcactgtt ataacaccca cattcggttc
     1561 tattgtacaa aaagggaaat tagccgcttg ggctttagcg tttgataaac aattaaaaag
     1621 ggtcgatttt cccacgttgg gtagtccaac tattccacat tgtaaagcca tgatacttga
     1681 gttaaaattt tgagaagaca aaggtaataa aaaagacata acttttttca tttctttgct
     1741 atcgttcata tttattatga gcaatagtag cacaatatgt tgttgatacg atgctttttt
     1801 gctacttttg catcaatttc agaaatcttc ataatttgtc atgaacaaaa gcaccatcat
     1861 acgtttttct gcacttttta ttgcaatatt tgttgtagta acgtttttat ttgtatatga
     1921 acgtaaaaaa aacaacgaaa aagaggctga acttaaccaa ataattgcag aactagaaat
     1981 tagtaaacaa gaatcgcttg acgaatttga aaatttggca ctagaatacg aaaactatca
     2041 tttcaaacca ggcaatgact cgctcttgtt tttgatagaa aacgaaaaac aaaaagttaa
     2101 ccaactgctc gaagagttgc gtacagtaaa agctaccaat gctcgtcgca tacgagaatt
     2161 gcgcaatgaa ctgggtagcg taaggtcggt attgcaggtg tacgttaacc gagtagattc
     2221 gctaagcaat gtaaacaaaa aactagtacg cgaaaacaaa aaagtacaac aacaatacga
     2281 agaggtagtg caaacggcta aagaattgga agaaagagcc tcgcaattgg ataaaaaaat
     2341 aacccttgca gcgattttag aagcaagcaa tattcgccta aacaccttaa atgataaagg
     2401 aagagaaacg aaaaaaagaa gcagaatagc cgcttttgaa atatgtttct cgctatcaaa
     2461 aaaccaaacg actagcactg gtctaaaatc ggcatattta cgcatatcca acccacaagg
     2521 aaacgtaatg aaaaataacc aaagtagtgt atttgctttc gaggataaag aaattgattt
     2581 ttcgtgcaaa aaggaaattg aatatacagg aaacaacctt tctacttgcg tttactacaa
     2641 cattgccgac gaagaactag tagctggctt atacgaagta actattttta ttgatggtaa
     2701 cttaattggt tcagaaagtt tttacattaa ataaaacact gtggcaggta tttatataca
     2761 tataccattt tgcaaacaac ggtgtggata ttgcgatttt tattcgcata ccaacttaca
     2821 actcaaacaa cggtatgtag ataccttatg caaagaaatt gaa
//
LOCUS       DAOPUR010000178         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36291_length_2859_cov_2.744650, whole genome shotgun sequence.
ACCESSION   DAOPUR010000178 DAOPUR010000000
VERSION     DAOPUR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36291_length_2859_cov_2.744650"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKN90_03965"
     CDS             <1..385
                     /locus_tag="PKN90_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNZ85468.1"
                     /translation="PAFVVVIAAFVSIIQILMEAYLPELYASLGLFIPLIVVNCIVLG
                     RAEAFAAKNGVFHSALDGLGMGLGFSLALTLLGSTRELLGTGKIFNMFIYPEHYGMLI
                     FVLAPGAFIVLGFLIVFVTKLQTSK"
     gene            393..965
                     /gene="rsxA"
                     /locus_tag="PKN90_03970"
     CDS             393..965
                     /gene="rsxA"
                     /locus_tag="PKN90_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNZ85469.1"
                     /translation="MSYIIIFIAAVFVNNIVLSQFLGICPFLGVSKKINTALGMSGAV
                     AFVMTISTIITYLLQKYVLEQFDILFLQTIVFILIISALVQLVEIILKKVSPPLYQAL
                     GVFLPLITTNCTILGVAIMTIQKDYTLLEGVVFAISTALGFGLALVIFAGIREQLVFA
                     KIPKAMQGAPIALLVAGLLAMAFMGFTGIV"
     gene            992..>1300
                     /locus_tag="PKN90_03975"
     CDS             992..>1300
                     /locus_tag="PKN90_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85470.1"
                     /translation="MFNFGLLASTTPYIVMAFLCAMSYVWMLFDKKPADQIEYISDQQ
                     QLNFDIEHTNKNKATTYVVANDEFKTSPCKYETVDCINTKTNNIVCLSFLTAKLYSKT
                     C"
     assembly_gap    1301..1310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1483..>2859
                     /locus_tag="PKN90_03980"
     CDS             1483..>2859
                     /locus_tag="PKN90_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNZ85471.1"
                     /translation="MLVSFFVLADDKSTVELNEFVVNSLPKESAFTTQLATTNYTIGM
                     QQVEKLGVQSIKNLSAHIPNYYLPDYGSKITSSMYIRGIGSRMNEPAIGMYVDNVPYI
                     HRSAFDIMLHDIQQFQFLQGPQGTLYGRNTIGGLMNIYTISPLVYQGTRLNVSYAKYN
                     DANVSVSHYQKLNDSLGVSISGYVQHQDGHFTNMGRMEKNKSDEFGIRGKLEWKMKNN
                     WQSMFQISYDYTDQIAYPYAKFDTISKQIYPINYNEQGAYRRKMLSSGFTLQKITDNY
                     AFTSATGFQNITDRMDIDQDFTSDSIMVLQQNQQLKSITQEFVFRANNSNNWQWVNGL
                     FLFGASNTIDSPMTSQKGGIKMIQNFMDMAKMFNPNMPTITILNEEMLVAGLFSKPSI
                     GAALYHQSSYSFTNKFSVTAGIRLDYEKAFIDYDSEATLQTSVKLPFPFVPAFRVDST
                     YAVKGFKSK"
BASE COUNT          923 a          458 c          488 g          980 t
ORIGIN      
        1 tcctgcattt gttgttgtca ttgctgcatt tgtaagtatt attcaaatat taatggaggc
       61 ttatttacca gaattatatg ccagtttagg cttatttatt cctcttattg ttgtaaactg
      121 tatcgtatta ggtagagccg aagcttttgc tgccaaaaat ggcgtatttc attcagcctt
      181 agatggattg ggtatgggat tggggttttc gttggcatta accctattag gatcaacaag
      241 agaattacta ggaacaggta aaatatttaa tatgtttatt tatccagaac attatggaat
      301 gctaattttt gttttggcac ctggagcgtt tattgttttg ggattcttaa tcgtctttgt
      361 tacaaaacta caaacatcaa aataaataca tcatgagtta tatcattatt tttatcgcag
      421 ctgtttttgt aaacaacatt gtattatcac aatttttagg tatttgtccg ttcttggggg
      481 tatctaaaaa aattaacact gcccttggaa tgagtggtgc ggtagccttt gtaatgacaa
      541 tttcaaccat tattacgtat ttgctacaaa aatacgttct agaacaattt gatattttat
      601 ttttacagac cattgtattt atcttaatta tttctgctct ggtacaatta gtagaaatta
      661 tattaaaaaa agtctctcct cctctatacc aagcattggg ggtgtttttg ccattaataa
      721 ccaccaattg tactatttta ggagttgcaa ttatgacaat tcaaaaagat tacactttgc
      781 ttgagggggt tgtttttgcg atatctacag ctctcggttt tggacttgct ttagtgatat
      841 ttgctggtat tagagaacaa cttgtatttg caaaaatacc caaagcgatg caaggagcac
      901 ctatagcttt actagtagca ggcttattgg ctatggcatt tatgggattt acaggtattg
      961 tgtaatttct ataaaaaaaa gagtatgtat tatgtttaat tttggacttt tagcatctac
     1021 tacaccctat attgttatgg catttttatg tgccatgtct tatgtatgga tgttatttga
     1081 caaaaaacct gcagatcaaa tagaatatat ttctgaccaa caacagctta actttgatat
     1141 tgaacatact aacaaaaata aggcaactac gtatgtagta gctaatgatg agttcaagac
     1201 ttcgccttgc aaatatgaaa ctgttgattg tatcaatact aaaacgaata acattgtttg
     1261 tttgtctttt ttaactgcaa aattatatag taaaacttgt nnnnnnnnnn catttttatt
     1321 tctctagacc tcctcccttt tcttgttaat gtatttatac aagacactct gtgaaacgag
     1381 ttgttatttt tttgttgaca tttaactatt tgtcaatcaa ccattttatt aacattaaca
     1441 tactattttt ttatgaaaat tattagtact gcatgtcttc tcatgctagt atcctttttc
     1501 gttttagctg acgataaaag taccgtagaa ctcaacgaat ttgttgtaaa cagtttgcca
     1561 aaagaatcag catttacaac acaattggct actacaaatt ataccattgg aatgcaacaa
     1621 gttgaaaaac ttggggtgca atcaataaaa aatctatctg cccatattcc caattattac
     1681 ttaccagatt atggctcaaa aatcacgtca tccatgtata ttagaggtat agggagtaga
     1741 atgaatgaac ctgctattgg aatgtatgtg gacaatgtgc cttatattca tcgtagtgct
     1801 tttgatatta tgttgcatga tatacagcag tttcagtttc ttcaaggtcc gcaaggaacc
     1861 ttatatggca gaaatacgat tggagggttg atgaatatat atacgataag ccctcttgtt
     1921 taccaaggaa caaggttaaa cgtatcgtat gccaaataca atgatgccaa tgtgtctgta
     1981 tctcattacc aaaaattgaa cgattcattg ggcgtaagta tctctgggta tgttcagcac
     2041 caggatggac attttaccaa tatgggacga atggagaaaa ataaatcaga tgaatttggt
     2101 attagaggaa aactggaatg gaaaatgaaa aacaattggc aatcaatgtt tcaaatttct
     2161 tacgattaca ccgaccaaat agcctacccg tatgccaaat ttgacacaat aagcaagcaa
     2221 atatatccta ttaactacaa cgaacaaggt gcatatagac gtaaaatgtt atcttctgga
     2281 ttcacacttc aaaaaataac ggataattat gcatttactt ctgcaactgg ttttcaaaac
     2341 attacggata gaatggatat agatcaagat tttacttccg actctattat ggtattacaa
     2401 caaaaccaac agttaaagag catcacgcag gaatttgtat ttcgtgcaaa taattcaaat
     2461 aattggcaat gggtaaatgg cttattttta tttggagcaa gtaatactat cgattctccg
     2521 atgacatccc aaaaaggtgg tataaagatg atacaaaact ttatggatat ggcaaaaatg
     2581 tttaatccca atatgcctac aattactatt ttaaatgaag aaatgcttgt tgctggatta
     2641 tttagtaaac catctattgg tgcagctttg taccaccaat caagctattc attcaccaat
     2701 aaattcagtg taaccgctgg aattcgattg gattatgaaa aagcatttat cgactacgat
     2761 tcggaagcaa ccttgcagac aagtgtaaaa ttacctttcc catttgttcc tgcttttcgg
     2821 gtggactcaa catatgccgt aaaaggcttt aaaagcaaa
//
LOCUS       DAOPUR010000179         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36434_length_2850_cov_2.758855, whole genome shotgun sequence.
ACCESSION   DAOPUR010000179 DAOPUR010000000
VERSION     DAOPUR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36434_length_2850_cov_2.758855"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..642
                     /locus_tag="PKN90_03985"
     CDS             52..642
                     /locus_tag="PKN90_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ85472.1"
                     /translation="MKRQKGFTLVELLVVIAIIGILAAILLPSLGSARTRAIRSKVSA
                     TISQLNQAVKAYQLDYGLYPPTIAPDPAHFNYSATTFVPCLDGDTTTTADGFGNSRII
                     YYEFEPKSVVGNTYFDPFMSPYLYLNCHILNLQNRVTTSYLPEIARYNYRSFQIFSKA
                     NYPNNTDDGSGSGPHGDAPENMRNMDTFKWITNYLN"
     gene            662..1297
                     /locus_tag="PKN90_03990"
     CDS             662..1297
                     /locus_tag="PKN90_03990"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ85473.1"
                     /translation="MKLKKGFTLVELLMVIAIIGILAGIVLSVGRGMVAKARIKSTYG
                     TIMALTSACEQFKAIYYRYPNVETGEGGCVGRTNNNQKLVHALEEFDPSSSDSDIVKQ
                     DPVLTQNLPKILKGTYEEYIDAWGNSLYVEAGRDHSNDIPRGPNNYNEKKPDFRWGTP
                     LDIYSSGPDGILQISKGGTTTQHNFKNAGNDDIASWMPLTRLVEEQDADGK"
     gene            1321..1956
                     /locus_tag="PKN90_03995"
     CDS             1321..1956
                     /locus_tag="PKN90_03995"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ85474.1"
                     /translation="MKQTKKGFTLVELLVVISIIVIMAAMAAPALSDVMSKRHLNGAA
                     TAIQMACMETRSRAIAQRERQVLAFALFRNNNSDNIINDEVTLNKITISTVTMSPAPK
                     VRRNTISSYDSNETATTPAFIPAGQPTEISEHVKWQTPDVNAFYLIFNPDGTIMFTDI
                     TNQNNDPNDLNNPHCDDTDIILVQNNLTERCFIDINHNTGKVRTAIREPRQ"
     gene            2036..2752
                     /locus_tag="PKN90_04000"
     CDS             2036..2752
                     /locus_tag="PKN90_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ85475.1"
                     /translation="MKYIHKGFTLVELMVAIGLMVLLMGVVVTIFSSSSEVMALAEAK
                     MGIYQNARAAFDLMARDITNMTGTPLAPNEVLNINNPNAGGLLLTFHTVTSWIDGNTQ
                     RTGAAMVQYTLIPSRTFNSQPLFSLQRTIIPADNPSNPITDVFAEFLVPPYVINMDPL
                     PDTDNPAPTMNIPFLTEFFTHNNNETANWSINNGAANPPGDTKLPTLPPAVRITMTLT
                     DEFMRARRTLSRVIWIPRGQ"
BASE COUNT          989 a          488 c          503 g          870 t
ORIGIN      
        1 agcatttgct gacaccttat ttcaaaataa tggtaaagaa aggaaatata tatgaaacga
       61 caaaaaggtt ttactttagt agaactctta gttgtgattg ccattatcgg cattctagca
      121 gctattctat tgccttctct aggaagtgca agaacaagag caatacgttc taaagtttct
      181 gctacgatca gtcaattaaa ccaagcagtc aaagcatatc aattagatta tggactgtat
      241 ccacctacta ttgctccaga tccagctcat tttaattatt cagcaacaac atttgttcct
      301 tgtttagatg gcgatacaac tacaacagct gatggatttg gtaattcaag aattatttac
      361 tatgaatttg aaccaaaaag tgtagttggt aatacatatt ttgatccctt tatgtctcct
      421 tatttatatt taaattgtca tatattaaat ttacaaaaca gagttactac tagttattta
      481 cctgaaatag cacgttataa ttatcgttct tttcaaattt tcagtaaagc aaattaccct
      541 aataatacag atgatggatc aggatcgggt cctcatgggg atgcacctga aaatatgaga
      601 aatatggata catttaaatg gatcacaaat tatctaaatt aatggatact aggagaaaac
      661 catgaaacta aaaaaaggat ttactctagt agaattgcta atggtaatag ctattattgg
      721 tattttagct ggcattgttc tttctgtagg gagaggtatg gttgcgaaag caagaattaa
      781 gtctacgtat ggaactatta tggcattaac aagtgcttgt gaacaattca aagctattta
      841 ttatcgctac cctaatgtag aaactggaga aggcggatgt gtaggtagaa cgaataataa
      901 tcaaaaatta gttcatgctt tagaagaatt tgatccctct agcagtgact cggatatcgt
      961 aaaacaagat cctgttttaa cacaaaattt gcctaaaata ctgaaaggta catatgaaga
     1021 gtatatcgat gcttggggga attcattgta cgtagaagct ggacgtgatc attctaacga
     1081 tattccacgt ggtcctaata attataacga aaaaaaacca gattttcgtt ggggtactcc
     1141 tttagacata tattcttcag gtcctgatgg tatactccaa atttcaaaag ggggaacaac
     1201 tacacaacat aattttaaaa acgctggtaa tgatgacatt gccagttgga tgccactcac
     1261 aagactcgta gaagaacaag atgcagatgg taaataactc attcaaaaaa ggtatatagc
     1321 atgaaacaaa caaaaaaagg ttttacatta gtggaattac ttgttgtcat ttccattatt
     1381 gtaatcatgg cagccatggc tgctcctgct ttgagtgatg ttatgagcaa acgtcactta
     1441 aatggtgctg ccactgccat tcaaatggct tgtatggaaa ctcggtcgcg agctattgca
     1501 caacgggaac gacaagtatt ggcttttgca cttttcagga ataataattc tgataatatt
     1561 ataaacgatg aggtaactct taataagata acaatctcta ctgtaactat gagtccagcc
     1621 cccaaagtaa gaagaaatac cattagttcc tatgattcca acgaaactgc tacaactcca
     1681 gcatttatac cagcaggaca acctactgaa atttcagaac atgtaaaatg gcaaacaccg
     1741 gatgtgaatg cattttattt gatttttaat cctgatggaa cgattatgtt tacagatata
     1801 acaaaccaaa acaatgatcc taatgatctt aataatcctc actgtgatga tacagatatt
     1861 atactagttc aaaataacct tacagagaga tgttttattg atattaatca taatactggt
     1921 aaagtaagga ctgccattcg agaaccacga caataattaa tagaattaag taagataatt
     1981 ttaacaatga ttttggaggg ttgcgttttc ttatagaaga caacgcttca tttttatgaa
     2041 atacatacat aaaggtttta ctctagtaga attaatggtt gccattggct taatggttct
     2101 tttaatgggg gttgtagtca ctatttttag tagttcctct gaagtgatgg cacttgcaga
     2161 agccaaaatg ggaatctatc aaaatgctcg tgcggccttt gatcttatgg cacgagatat
     2221 taccaatatg actggtacac cattggctcc taatgaagta ttgaatatta ataatccaaa
     2281 cgctggtggt ttattactga catttcacac agtaacttct tggatagatg gtaacacaca
     2341 acgaacaggt gcagccatgg tacaatatac attaatccct tcacgcacat ttaatagtca
     2401 accactcttt agtttgcaac gaacaataat accagctgac aaccccagta atccaataac
     2461 agatgttttt gctgaatttt tagttccacc ttatgtgata aatatggatc ctctaccaga
     2521 tactgataat cctgcaccta ctatgaatat tcctttttta acagagtttt tcacacataa
     2581 taataatgaa actgcaaatt ggagtataaa taacggagca gcaaatccac ctggggatac
     2641 taaattgcct acacttccac ctgctgtacg catcactatg acattgacag acgaatttat
     2701 gcgagcacgt agaacattaa gtcgagtgat atggattccc agaggacaat aaaacatagg
     2761 agccaacaat gaaacaaaca caaaaaggtt ttacattaat tgaaatatta atttccatgg
     2821 gaattttagc attaggagtt tcaggaatct
//
LOCUS       DAOPUR010000180         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36447_length_2849_cov_3.018969, whole genome shotgun sequence.
ACCESSION   DAOPUR010000180 DAOPUR010000000
VERSION     DAOPUR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36447_length_2849_cov_3.018969"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PKN90_04005"
     CDS             <1..1027
                     /locus_tag="PKN90_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ85476.1"
                     /translation="NLTNLLNVYRKSWRFNVYANLGLGASFYSVEPSNIVSPTNIPHA
                     TTYTRTPNEIEDGRSLLVPMSLNFEYNISKPLAVGLNVHYRATNKDNYEGEAYVRGVQ
                     NDNFLLGTASLRYKFASGQKDGHIRNITADKFRQEETKEREILDKLDSLNNKVDDLDS
                     VVTNDLTPRMNEVERLLSDQPDADGDGVPDWRDREPNTPAGSFVNYWGESLPYEVLKA
                     TEFYEELAKMQEEEVDYDMSVYFAFDKKNLTKRSIDNIAKAAQKLKSNPLLKVELRGY
                     ADYPGSHNYNLRLSTSRGEVVKQELVNKHGIDPSRITLNPQGRMTNPPNANQKNRRTD
                     FVFSKMK"
     gene            1107..1490
                     /locus_tag="PKN90_04010"
     CDS             1107..1490
                     /locus_tag="PKN90_04010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNZ85477.1"
                     /translation="MEEKEHIHYDQFAKNSGIKLVEAKNGEAIAKMEIRNMHLNGLNL
                     VHGGALFTLADFTLGAAVNSLGGAHVTMSASIHYVKSAKEGTLIGISKQVSAGRTIAT
                     YDVEIRLEETNDLIAIVRGNSFKKS"
     gene            1577..>2849
                     /gene="cysS"
                     /locus_tag="PKN90_04015"
     CDS             1577..>2849
                     /gene="cysS"
                     /locus_tag="PKN90_04015"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNZ85478.1"
                     /translation="MEHTLQIYNTLTRKKEVFIPIHSPNVGLYVCGPTVYGDAHLGHA
                     RPAITFDLLFRYLTHLGYKVRYVRNITDVGHLEHDADEGEDKVAKKARVEQKEPMEIV
                     QFYLNRYRKAMEKLNVLPPSIEPHASGHIMEQIEFTQKIIDAGFAYQSEGSVYFDVEK
                     YNKQYHYGKLSGRNIEELLETTRTLDGQSEKQKSVDFALWKKASPEHIMRWKSPWSDG
                     FPGWHMECSAMGTKYLGEEFDIHGGGMDLVFPHHECEIAQSTAALGKETVRYWMHNNM
                     ITINGQKMGKSLGNFITLDEFFGGSHPLLSQAFSAMTIRFFILQAHYRSTVDFSNEAL
                     QASEKGLNKLLETYARLQKITPSATSTIDVSGLRAKCEEAMKDDLNTPIVLSHLFEAS
                     KAINTVADGKASLSAKDVAELKSVFKLFVEDI"
BASE COUNT         1024 a          477 c          566 g          782 t
ORIGIN      
        1 caatttaacg aatttgttaa atgtttatcg caaatcatgg agatttaatg tttatgcaaa
       61 tttaggatta ggagcttctt tctacagtgt tgaaccctcg aatattgttt ctcctacaaa
      121 tattcctcat gcaacaactt acacaagaac tccaaatgaa attgaagatg gtcgttcgtt
      181 gttagttcct atgtctttga actttgaata caacatttct aaaccattag cagtgggttt
      241 aaatgttcac tatagagcaa caaacaaaga taattatgaa ggagaagcct acgttagagg
      301 cgttcaaaat gataatttct tattaggaac tgctagttta cgttataaat ttgcttctgg
      361 tcaaaaagat ggtcatatca gaaacataac tgctgataaa tttagacaag aagaaactaa
      421 agagagagaa attcttgaca agcttgattc attaaacaac aaggtagatg atcttgatag
      481 tgttgttaca aatgatttaa ctccacgcat gaacgaggta gaaagattac tatctgacca
      541 accagatgct gatggcgacg gagttccaga ttggagagat agagaaccaa atacacctgc
      601 aggttcgttt gtaaactatt ggggagaaag ccttccttat gaagtattga aagctactga
      661 gttttacgaa gaattagcta aaatgcaaga ggaagaagtt gattacgaca tgtctgttta
      721 ctttgcattt gataaaaaga acttgacaaa acgttctatc gacaatattg caaaagcggc
      781 tcaaaaatta aaatcaaacc cattattgaa agtagaactt agaggatatg ctgactaccc
      841 tggtagccac aactacaact tacgtttgag tacttctcgt ggtgaagttg ttaagcaaga
      901 gttagtaaac aaacatggta ttgatccttc aagaatcacc ttgaatccac aaggaagaat
      961 gaccaatcca ccaaacgcta accaaaagaa cagaagaact gactttgtgt tcagcaaaat
     1021 gaaataaaaa gattgtagtc tttaattaat agaaaaagcc ctgagtcaac atgacgtcag
     1081 ggttttttta acaataacaa catataatgg aagaaaaaga acatattcat tacgaccaat
     1141 ttgcaaaaaa ctcgggtatt aaattagtcg aagcaaaaaa tggagaagca atcgcaaaaa
     1201 tggaaataag aaacatgcat ctaaacggct tgaatctggt gcatggtgga gccttattta
     1261 ccttagcaga tttcacttta ggggcagccg tcaactctct tggaggagct catgtaacga
     1321 tgagtgcaag tattcactat gtaaaatctg ccaaggaagg aacacttatt ggaatatcta
     1381 aacaggtttc tgcaggtaga actatcgcaa catacgatgt agaaatacga ttagaagaaa
     1441 caaatgactt aattgcaatc gttagaggaa attcgtttaa gaaatcttag aaatatgaag
     1501 aattcttgta taaaagcata attatagtag aataaaatat gtaattttgc aggaaagaaa
     1561 acaatcagtt gaaattatgg aacatacatt gcaaatttat aacacactaa ctcgaaagaa
     1621 agaagttttt atacccattc atagcccaaa tgttggcttg tatgtatgtg gaccaactgt
     1681 atatggcgat gctcatttag gtcatgcacg acccgctatt acttttgatt tactgtttcg
     1741 ttacttaacc catttaggct ataaggtgcg ttatgtacgc aacataacag acgtaggaca
     1801 tcttgagcat gacgcagatg aaggagaaga taaggttgca aaaaaagcta gagtagaaca
     1861 aaaagaaccc atggagattg ttcaattcta cctaaataga taccgcaaag ccatggagaa
     1921 attgaatgta ttgccaccaa gtattgagcc acatgcgtct ggacacatta tggagcaaat
     1981 tgaatttaca caaaaaatta ttgatgcagg ttttgcctac caaagtgagg gttcggtata
     2041 ttttgatgta gaaaaataca acaaacagta ccactacgga aaattgtcag gaagaaatat
     2101 agaagaactt cttgaaacaa cacgaacact tgacgggcaa agtgaaaaac agaaaagtgt
     2161 ggattttgcc ttgtggaaaa aagcatcgcc agaacatatt atgcgatgga aatcgccatg
     2221 gagcgatggt tttccgggct ggcacatgga gtgttcggct atgggcacga agtacttagg
     2281 agaagaattt gacatacatg gcggaggtat ggatttagtt tttcctcatc atgagtgcga
     2341 aattgcacaa tcgacagccg ctttaggcaa agaaactgta cgttactgga tgcacaacaa
     2401 tatgattacc attaatggcc agaagatggg caaatcgcta ggtaatttta ttacattaga
     2461 tgagtttttt ggaggttcgc atccactgct ttcacaagca ttttcagcca tgaccattcg
     2521 cttttttatt cttcaagcac attatcgtag caccgtagat tttagcaacg aagcattaca
     2581 agcttctgaa aaaggactta acaagttgct tgaaacgtat gcacgcttgc aaaaaataac
     2641 tccttcagcc acatctacta tcgatgtatc tggtttgcga gctaaatgcg aagaggctat
     2701 gaaagatgat ctaaatacac cgatagtatt atcacatctt tttgaagcct caaaagccat
     2761 taacactgtg gcagatggaa aagccagctt aagtgcaaag gatgtagcag aactaaaatc
     2821 agtattcaag ttatttgttg aagacatac
//
LOCUS       DAOPUR010000181         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36564_length_2842_cov_3.310370, whole genome shotgun sequence.
ACCESSION   DAOPUR010000181 DAOPUR010000000
VERSION     DAOPUR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36564_length_2842_cov_3.310370"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(643..>2842)
                     /locus_tag="PKN90_04020"
     CDS             complement(643..>2842)
                     /locus_tag="PKN90_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNZ85479.1"
                     /translation="PTAGLELSQEIRKLDPFVPIIVESSEPKGVCEQTDRLCAIFLDK
                     TDKTFHKQFKDAITHHFKFGDLVFVSPDTREALITVHNLQDLQKHIFDIPANSLFYHL
                     AQNDLSRWLYSRAMFPLAEYLRYIVIKDIKDIDRARHIVFDAIVNYRKIKNKGIVAVF
                     QSDRFDEYSNFARIGEDSMGGKGRGLAFLNAVSKRNPTLYDFENTQITVPKTVVLCTD
                     MFDEFMETNNLYVVALREDVSDEQILEVFLEARLPRKIMKDLYAFLGVIQSPIAVRSS
                     SLLEDSHYQPFAGVYSTYMVPNVSESRTTTLRMVCDAIKAVYASVFYADSKAYMKATK
                     NLIDEEKMAVVLQEVCGNAYGNRFYPSFSGVARSLNYYPIGEEKPEEGVVNVALGLGK
                     YIVDGGQTLRFCPAYPNKVLQTSTMEFALSETQNYFYCLDVAQRSFKPRVDDGDNLQK
                     LHIREAEQDGTLRWLASTFDPIDQVIRDGVYAGGRKLITFVNILEHQSFQLADILKNV
                     LTVGEKEMGCPVEIEFAVHLDYEKNEQHTFYLLQIRPIVGLNESLTEDISLISKEKTF
                     LSTTNALGNGIMNDVYDVVYVKKGAFNPSKNILIAYDIEKINKQLVAEDKSYILVGPG
                     RWGSSDHWLGIPIKWPHISGARVIVEAGETSYHIDPSQGTHFFQNLTSFGVCYFTITQ
                     NEDDYYDCAYLDEQEALHETEYIRHVRFSSPLLVKVDGRKNRGVVLKVES"
BASE COUNT          927 a          591 c          436 g          888 t
ORIGIN      
        1 cttgatacaa aaaacgaagg aaaaaaaatc aagactgcga ttattcagtg gaaagtgtat
       61 agttaaaagt tgaaagtgtt gggagagaaa cgttgttggt ggttcgtgcg aaactatcaa
      121 ctgtcaattc tcaactatca actcaataaa aactcgctcc gataaatcgg atctcaaaca
      181 gcattctttt ttaaccgctc atttcacggc attttttggc tacataaacg atgtcgtcgc
      241 gggctattca gttgatagcg gaaaatgtgt aaggtttagc gttgtttgat tctcgttggg
      301 cgtttaatgt gtagcgtgta acgtgtaggg gttagtggta aaattgtcag ttaaaaagtt
      361 gaaagtaaac ccaacagcaa caacacctaa actttctaac tttctaaact ctctaacttt
      421 caacagaatt aggagcgtag ctctgatatc ttcgtagaaa accaaaaaaa aaccgataag
      481 tgaggggcgt tagccctgat atcttagttg atagttgaca attgacagtt aaaagttata
      541 aagttgaaag tataaagtaa acccaacaac aacacccctc ccaaaaaaac tttctaactt
      601 tatgaacttt ttaacttttt aactttctaa ctttatgaac tgtcaactct ccactttcaa
      661 aaccacccct ctgttctttc tcccatcaac ttttacaagt aatggagatg aaaatcgtac
      721 atgacgtata tattctgttt cgtgcaaagc ttcctgttcg tccaaataag cacaatcata
      781 atagtcatct tcgttttgag ttatcgtaaa ataacacaca ccaaaagacg ttaaattctg
      841 aaaaaaatga gtaccctgac ttgggtcgat atgatacgat gtttctccag cttcaacaat
      901 aacgcgtgct cccgaaatat gtggccactt aataggaata cctaaccaat ggtcactact
      961 tccccagcga ccaggaccaa ccaatatata agatttatct tcagcaacta attgcttatt
     1021 tattttttcg atatcataag ctatcaagat atttttagaa ggattgaaag ctcctttttt
     1081 aacgtatact acatcataga catcattcat aatgccattt cctaaagcat tagtagtcga
     1141 gagaaaagtt ttttcttttg aaataaggct tatatcttct gttaacgatt catttaagcc
     1201 tacaattgga cgtatttgaa gtaaataaaa ggtgtgttgc tcattttttt cataatctaa
     1261 atgtacggca aattcaattt ctactggaca tcccatttct ttttcgccaa ccgtcaatac
     1321 atttttcaat atatccgcca attgaaaaga ctgatgttct aagatattta caaaagtgat
     1381 taacttgcgt ccacctgcat atactccatc acgtattact tggtcaatag gatcaaaagt
     1441 agatgctaac caacgtaatg taccatcttg ttcagcctct ctaatatgta gtttttgcaa
     1501 attatcgcca tcatctactc ttggtttaaa cgacctttgt gctacatcca aacaataaaa
     1561 ataattttgt gtttcactaa gagcaaactc catggtactc gtttgaagca ccttatttgg
     1621 atacgctgga caaaaacgta atgtttgccc tccatcaaca atgtatttac ctaaacctaa
     1681 agccacatta acaacaccct cttctggttt ttcttcgcca ataggataat aatttaacga
     1741 tcgtgccact cccgaaaaag aaggataaaa ccgatttcca taagcatttc cacacacttc
     1801 ttgtaacact acagccattt tctcttcatc tatcaggttt ttagtggctt tcatgtatgc
     1861 tttgctatct gcataaaata cagatgcata taccgcttta atagcatcgc acaccatccg
     1921 taaagtagta gtacgactct ctgatacatt cggaaccata taggtggagt aaacccccgc
     1981 aaaaggctga taatgagaat cttctagtaa gctagacgat cgaactgcaa tcggactttg
     2041 aattacccct aaaaaagcat acaaatcttt catgattttt cgtggcaatc ttgcctccaa
     2101 aaaaacctct aaaatttgtt catcggatac atcttctcgc aacgctacta catacaaatt
     2161 gtttgtttcc ataaactcat caaacatatc tgtacaaagc acaactgttt taggaacagt
     2221 tatttgcgta ttttcaaaat catacaaagt tggattacgt tttgaaacag cattcaaaaa
     2281 tgctaaccct ctcccttttc ctcccatcga atcttctcct attcgtgcaa aattggagta
     2341 ttcatcaaat ctatcgcttt gaaaaacagc cacaatacct ttgtttttaa tcttgcgata
     2401 atttacaatc gcatcaaaca caatatgtct tgccctgtca atatctttta tgtctttaat
     2461 aacgatataa cgcaaatatt cagctagcgg aaacatcgcc cgtgaataga gccatcgaga
     2521 taaatcattt tgagccaaat gataaaacaa agaatttgca ggaatatcaa aaatatgttt
     2581 ctgcaaatct tgcagattat gtaccgttat caaagcttca cgggtgtcag gagacacaaa
     2641 taccaaatcg ccaaacttaa aatgatgcgt tatagcgtct ttaaattgtt tgtgaaaggt
     2701 tttgtctgtt ttatctaaaa aaatggcaca cagcctgtct gtttgctcac aaacgccctt
     2761 tggctcactt gactcaacta taatagggac aaacggatcc aactttctaa tttcctgact
     2821 taactccaat ccagcggttg ga
//
LOCUS       DAOPUR010000182         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36824_length_2829_cov_2.571738, whole genome shotgun sequence.
ACCESSION   DAOPUR010000182 DAOPUR010000000
VERSION     DAOPUR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36824_length_2829_cov_2.571738"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..563)
                     /locus_tag="PKN90_04025"
     CDS             complement(165..563)
                     /locus_tag="PKN90_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNZ85480.1"
                     /translation="MSNILVSGLINIETTLQCDEFPIDYSPVRYPFFGIQNTISGVGY
                     NVAKALAPLSDNVQFMSIIGKDKEGEWITDELQSLHIDTQYITPSIAQTPIGGLLSSL
                     ILTVFLVPMVYYVVDTIKERMNKKSKKIAE"
     gene            complement(598..1176)
                     /locus_tag="PKN90_04030"
     CDS             complement(598..1176)
                     /locus_tag="PKN90_04030"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNZ85481.1"
                     /translation="MKKILIINGNPDKESLSFELATAYKKGADASGAACTLVHLSDLQ
                     FDLNLKFGYRQRTELEPDLVMMQQAIKEADHLVFVYPTWWGTYPTLLKGFIDRVFLPK
                     FAFSYRENSPLWDKLLVGKTARLIVTMDTPTWYYKWIFKQPGHNSMKKGILEFCGIKP
                     VKITNFGSVKTSTEATRKKWLAEVENLGKKVS"
     gene            complement(1186..>2829)
                     /gene="lon"
                     /locus_tag="PKN90_04035"
     CDS             complement(1186..>2829)
                     /gene="lon"
                     /locus_tag="PKN90_04035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNZ85482.1"
                     /translation="EYFLQQQMKTIQKELGGNVQDDLINDIKKKAEGKKWDEKVRKIA
                     EKEIAKLETTSTHSSDYSVQLNYVQTLVDLPWNEYTKDNFNLKRAQRVLNRDHFGLDE
                     VKDRIIDHLAVLKLKGDLKSPIICLYGPPGVGKTSLGKSIADALGRKYARISLGGLHD
                     EAEIRGHRRTYIGAMPGRVIQNILKTNSSNPVLVLDEIDKVGRDYKGDPGSALLEVLD
                     PEQNFAFHDNYLDVDYDLSKVLFIATANSLSSISQPLLDRMELINVTGYIVEEKIEIA
                     MKHLIPKQLENHGIPKDAVSFSKKTVQEIVEKYTRESGVRNLEKQIAKICRRLARKIA
                     MEEKYDTSMPISDLKEYLGTEKYTRDKQSQKEEIGIVTGLAWTSVGGEILEIETSLSK
                     GKGGKLTLTGNLGDVMKESAMLGLEYIRAHAKDFGLNEDLFEETNVHIHVPEGAIPKD
                     GPSAGITMATSLLSAFTKRKVKKHIAMTGEITLHGKVLPVGGIKEKILAAKRAGITDI
                     ILSHENKKNIDEINAIYIKGVTFHYVKDIKEVIQIALLK"
BASE COUNT          775 a          574 c          492 g          988 t
ORIGIN      
        1 aggcaaaaac gacattcccg tttgaatttc tcaactcact cacacttact gtgagtgtaa
       61 ttcctttatt atctgctgct tgaataacag agccgataga taaaagcaga attaagcaaa
      121 ttaaataatg cttcataaat gatatatttt ttttgataaa catcttattc tgctatcttt
      181 ttcgattttt tattcattct ttcttttatt gtatctacta cgtaatacac cattggcaca
      241 aggaatactg taagaattaa cgaagataat aatccgccga taggtgtttg agcgatgctg
      301 ggtgtaatgt attgtgtgtc aatgtgtagc gattgtaatt cgtcggttat ccattcgcct
      361 tctttgtctt ttccgataat agacataaac tgaacattgt cggatagcgg ggcaagagct
      421 ttagctacat tgtagcccac gcccgaaatg gtattttgaa taccaaaaaa tgggtaacgc
      481 actggcgaat agtcaatcgg aaactcatcg cattgcaagg tcgtttctat gtttattaaa
      541 ccagatacaa ggatattgct catgccttac ttcgatttgg ttactatacg taggagctta
      601 ggataccttt tttcctaaat tttcgacctc tgccaaccat tttttacgtg ttgcttctgt
      661 tgaggttttc acgctaccaa agttggtaat ttttacaggt ttgataccgc aaaactccaa
      721 aatgcctttt ttcatggagt tatgtccggg ttgtttaaaa atccatttat agtaccaagt
      781 aggtgtatcc atggttacaa tcaatcgagc cgttttacct accaataatt tatcccatag
      841 gggcgagttt tcgcggtacg agaatgcaaa ttttggtaaa aatacacggt cgataaaccc
      901 tttgagcaag gtagggtagg ttccccacca agtaggatat acaaatacca gatggtcagc
      961 ctctttaatg gcttgttgca tcattaccaa atcgggttct agttcggtgc gttgacggta
     1021 gccaaatttt aggttcaaat caaattgcaa gtcgcttaaa tggactaagg tacaagctgc
     1081 accactggcg tcagctcctt ttttgtacgc agtagctaat tcaaagctca aactttcttt
     1141 gtcaggattg ccgttgatga ttaaaatttt tttcatacat cccacttatt ttagtaaagc
     1201 aatttgaatc acctctttaa tatcttttac ataatgaaac gtaacccctt tgatataaat
     1261 ggcattaatt tcatcaatat tctttttgtt ttcgtgcgac aaaataatat ctgtaatacc
     1321 agcacgtttg gcagccagaa ttttttcttt aataccacct acaggcaaca cttttccatg
     1381 taaggtaatt tctccagtca tggcaatatg tttttttact tttcgtttgg taaacgcaga
     1441 taacaagcta gtagccattg taataccagc agaaggtcca tctttcggta tagcaccttc
     1501 gggtacatga atgtgtacgt tggtttcttc aaataggtct tcgttaagtc caaaatcctt
     1561 tgcatgagca cggatatatt ccaacccaag catggcagat tctttcatca cgtcgcctaa
     1621 attgcccgta agcgttagtt tgccaccttt gcctttgcta agactggttt caatctctaa
     1681 aatttcgcca cctactgatg tccaagccaa accagtaaca ataccaattt cttctttctg
     1741 actttgctta tctctggtat atttttctgt tcctaaatac tcttttaaat cgctaattgg
     1801 catagatgtg tcgtattttt cttccatggc aattttgcga gccagtctgc gacaaatttt
     1861 tgcaatttgt ttttctaaat tacgtacacc cgattcgcgt gtgtatttct ctacaatttc
     1921 ttgtaccgtt tttttagaaa atgaaacagc gtcttttgga ataccatgat tctctaattg
     1981 tttaggaatc aggtgtttca ttgctatttc aattttttct tctacaatat agccagttac
     2041 gttaatcaac tccattctgt cgagcaatgg ttgcgaaata ctactcaagc tattggctgt
     2101 ggcaataaat aatactttag acaaatcgta atctacatct aaataattat cgtggaaggc
     2161 aaagttttgt tctgggtcga gcacttcgag caaagcagag cctgggtcgc ctttgtaatc
     2221 gcgaccaact ttatctattt cgtctaatac caaaacagga ttagatgagt ttgtttttaa
     2281 aatattttgg ataacacgtc caggcatagc tccaatgtac gtgcgacggt gtccacgaat
     2341 ttcagcttca tcgtgcaatc cacccaacga tatacgtgca tattttctgc ccaatgcatc
     2401 agcaatggat tttcctaacg atgtttttcc aacccctgga ggtccataca agcaaatgat
     2461 aggcgatttt aaatctcctt ttaatttaag tacagccaaa tggtcaataa ttctgtcctt
     2521 tacttcgtct aatccaaaat ggtctctatt cagcacacgt tgggcacgtt tcaggttaaa
     2581 attatctttg gtatattcat tccaaggcaa atcgactaag gtttgcacat aattcagttg
     2641 taccgaataa tcgctcgaat gggtgcttgt ggtttctagt ttcgcaattt ctttctcggc
     2701 aattttacgg actttttcat cccatttttt tccttcggct ttcttcttaa tatcgtttat
     2761 caagtcgtct tgcacattgc cacccaattc tttttgaatg gttttcattt gttgctgcaa
     2821 aaaatattc
//
LOCUS       DAOPUR010000183         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36888_length_2825_cov_3.016245, whole genome shotgun sequence.
ACCESSION   DAOPUR010000183 DAOPUR010000000
VERSION     DAOPUR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36888_length_2825_cov_3.016245"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1068)
                     /locus_tag="PKN90_04040"
     CDS             complement(64..1068)
                     /locus_tag="PKN90_04040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNZ85483.1"
                     /translation="MMDGKSLSITEDKQNALRQLFPEVFSEGKIDWEKLKATLGEDIT
                     FSNERYVLNWAGKSDAFKVLQAPTTKTLIPAKDESINFDETENIFIEGENLEVLKVLQ
                     KSYFGKIKMIYIDPPYNTGNDSFIYPDKFAETKEEYQKRVGDKDEDGYMTKDGMFKKN
                     SKENGQYHSNWLNMMYPRLFLAKNLLRQDGVIFVSIDDNEVHNLRLLMNEIFGEENFE
                     ATIIPIVNPGGRDYKQVAITNEYILCFSKTDDLELNEINKVVSFKFNDSNGGFEFREL
                     RNRNPKFHSGNRPNLFYPFFVNPNVIDENGYCNVSTEKSDDFYIEVKPYNSEGNESVW
                     "
     gene            complement(1312..2718)
                     /gene="asnS"
                     /locus_tag="PKN90_04045"
     CDS             complement(1312..2718)
                     /gene="asnS"
                     /locus_tag="PKN90_04045"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNZ85484.1"
                     /translation="MEAIKRKKIVEALKSSSFGEKVNIKGWVRTKRGNKTVNFIAIND
                     GSTINNIQVVVDMEKFGEEILKPITTGACVSINGILTESLGKGQSVEIQATEIELYGA
                     ADPDTYPLQKKGHTLEFLREIAHLRPRTNTFGAIFRVRHQMAMAIHNFFHNKGFVYVH
                     TPIITGSDCEGAGQMFQVTTLNLYNLKKNEQGQIDYTDDFFGKQASLTVSGQLEGELA
                     AMALGEIYTFGPTFRAENSNTPRHLSEFWMIEPEMAFYEIADNMQLAEDFVKHCIQWA
                     LDNCQDDIQFLSEMYDKELVERLKFVVDNEFIRLTYTEGIEILENAVKNGKKFEFPVG
                     WGTDLQSEHERYLVETHFKKPVILTDYPKEIKSFYMKQNADGKTVRAMDVLFPKIGEI
                     IGGSQREENYEKLRQRAAEMGVPEKDIWWYLDTRRFGTAPHSGFGLGFERLLLFVTGM
                     ANIRDVIPFPRTPNNAEF"
BASE COUNT          789 a          563 c          463 g         1010 t
ORIGIN      
        1 taacacttgc gttttatcaa agttatttac tacaatagct tcttttgctt taggttttcc
       61 ccatcaccaa acactttcat taccttcgct attataaggt tttacctcaa tgtaaaagtc
      121 atctgatttt tctgtcgaaa cattacaata accattttcg tctattacgt tgggattgac
      181 aaaaaatgga taaaataaat ttggtctatt tccactatga aacttaggat ttctgtttct
      241 taattctcta aattcaaaac ctccattgct atcattaaac ttaaaactta caaccttatt
      301 aatttcatta agttctaaat catcagtttt agaaaagcat aatatatact cgttggttat
      361 agctacttgt ttgtaatctc taccacctgg attaacaatg gggataatag tagcctcgaa
      421 attctcctct ccaaatattt cattcattaa taatcgtaag ttatgaactt cattatcatc
      481 aattgaaacg aaaattactc catcctgtct caatagattt ttcgccaaaa acaaacgagg
      541 gtacatcata ttcagccaat tgctgtggta ttgtccgttt tctttgctgt tttttttgaa
      601 catgccatcc ttggtcatgt agccatcttc gtctttgtcg cctacacgtt tttggtattc
      661 ttctttggtt tcagcaaact tgtcggggta aataaacgaa tcgttaccag tattgtaagg
      721 cgggtcgata tatatcattt taattttacc gaagtagctt ttttgcagta ctttcagcac
      781 ctcaaggttt tcgccctcta taaaaatgtt ttcggtttcg tcaaaattta tggattcgtc
      841 ttttgcagga attaaagtct ttgtagtagg tgcttgtaac actttaaaag catcgctttt
      901 accagcccag tttaacacat aacgctcgtt gctaaatgta atatcctcgc caagtgtggc
      961 tttcagcttt tcccaatcaa ttttcccttc gctaaatact tctggaaaaa gttgtcgcaa
     1021 agcgttttgt ttatcctctg taatagaaag acttttcccg tccatcatgt tatttgtgtg
     1081 tttttttcaa atttattgct attttcaacg tattcctatg ttgttttact ttttatttga
     1141 tgcaaaatat agccgtgcac caatatgtgc aaatgtacaa aaaatttctg ctgtaaaaaa
     1201 tatttagtta ggtaaaaata ttttctttca attattgggc taatccataa aaaagcccct
     1261 aaaatatttt tcaaatttag gagctttatt ttattcaaaa gagttttttc atcaaaactc
     1321 agcattatta ggagtgcgtg gaaatggtat tacatcgcgt atatttgcca taccagtaac
     1381 gaaaaggagc aatcgttcaa atcccaatcc aaagccagag tggggagctg taccaaagcg
     1441 gcgagtatcc aaataccacc aaatatcttt ttcgggcaca cccatttctg ctgcacgttg
     1501 gcgtagcttt tcgtagtttt cttcacgctg agaacctccg atgatttcgc caatttttgg
     1561 gaacaacaca tccatagctc gcaccgtttt gccatcggca ttttgtttca tataaaacga
     1621 cttaatttct tttggatagt cggtcaatat aacgggtttt ttaaagtgtg tttctaccaa
     1681 gtagcgttcg tgttctgatt gcaagtcggt gccccagccc acaggaaact cgaatttttt
     1741 accgttttta acggcatttt ctaatatttc aatgccttcg gtgtaggtca atcgaataaa
     1801 ctcattatct accacaaatt tcaaacgttc taccagttct ttatcgtaca tttctgataa
     1861 aaattggata tcgtcttggc aattatccaa agcccattgt atgcaatgtt ttacaaaatc
     1921 ttcggctaat tgcatattgt cggcaatttc gtaaaacgcc atttcaggct ctatcatcca
     1981 aaactctgat aggtgacgag gcgtattgga attttctgcc ctaaaagtag gtccaaacgt
     2041 gtaaatttcg cccaatgcca tggcagcaag ttctccttcg agctgacccg atacggttaa
     2101 actggcttgt ttgccaaaaa aatcgtcagt atagtcaatt tgtccttgtt cgttcttttt
     2161 aaggttgtat aagttgaggg tagtaacctg aaacatttgt cctgcaccct cgcagtcaga
     2221 cccagtaatg atgggagtat gtacgtatac aaatcctttg ttgtgaaaaa agttgtggat
     2281 agccattgcc atttggtggc gcactctaaa aatggctcca aacgtattgg tacgtggacg
     2341 taaatgagct atttcgcgca aaaactccaa ggtatgtcct ttcttttgca aaggatatgt
     2401 gtcggggtca gccgctccgt aaagttctat ttctgttgct tgtatttcaa cggattgtcc
     2461 cttccccaac gattcggtta atataccatt aatacttaca caagctcctg ttgtaatggg
     2521 ttttagaatt tcttctccaa acttttccat atccactacc acttgtatat tattgatagt
     2581 gctaccgtca ttaatggcga taaaatttac agttttattt cctcttttcg ttcttaccca
     2641 gcctttaata ttcactttct caccaaatga tgaggatttt agggcttcta caattttttt
     2701 tcttttgatt gcttccatct gctgtaaatg ataaaatttc gtaattaagc cacaaaggta
     2761 agtattttgc aattaattgt ggtaaaattg ccgttaaatt ctacataaaa aagtcccttt
     2821 gcggg
//
LOCUS       DAOPUR010000184         2821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_36966_length_2821_cov_3.118944, whole genome shotgun sequence.
ACCESSION   DAOPUR010000184 DAOPUR010000000
VERSION     DAOPUR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36966_length_2821_cov_3.118944"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PKN90_04050"
     CDS             complement(<1..997)
                     /locus_tag="PKN90_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ85485.1"
                     /translation="MKNLKFRLIVMSFLQFFVWGAWLITIAIYWFDTKQWDGAQFGAV
                     FSTLGISSLIMPTLMGIIADRWVNAEKLYGLLHLLSGLCIFSLAFVNSPVVFFWVMLL
                     AMFFYMPTISLSNSIAYNALKEVNLDIEKAFPPIRIWGTIGFIIAMWVVNLTGNKATA
                     VQFYISAVLSFCLAIYSFTLPACIPQRSKEKSSSIVQMLGLDAFKLFANPKMAIFFIF
                     SMFLGAALQLTNMYGDTFLDDFKNIEQYADSFVVRYSTIVMSISQISETLFILAIPFF
                     LKRYGIKNVMLMSMIAWVLRFALFGFGNPDDRLWMIILSMVVYGMAFDFFNISGSL"
     gene            complement(1003..1560)
                     /locus_tag="PKN90_04055"
     CDS             complement(1003..1560)
                     /locus_tag="PKN90_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNZ85486.1"
                     /translation="MIELYVFGVSLGISKTETHTLLLKEVAGSKYLMIEIGYSEAQSI
                     AIQLEQKKVPRPLIYDVIVNLVSNLNVAIERIVIDKLQDGIFFSKLVYRMGVHVLQSD
                     CRPSDGVAIAVRCGCPIFVSEEVLTVAMQQRLAIKTKKLADMTYEELKQEMQLAAERE
                     DYKQALLCKEEIIKRSTNNEEKNPN"
     gene            1726..2292
                     /locus_tag="PKN90_04060"
     CDS             1726..2292
                     /locus_tag="PKN90_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNZ85487.1"
                     /translation="MYKELIKTIALFIAKPVKAWEYINTKNPTENNPLHSYAYPLMGL
                     GSLSAFIVALVNNGSNDGFQIALKEASLLFISSFIGLYTAAFLIRIFYEKYCKEPLSF
                     DVTIQFVVYSSSVIFIINLFNQVTSSIFFLQIFSLYTIYIVWEGSIHFLKIEESKRAS
                     FVLFSAFCMILLPIAITALMKFFMPGLQ"
     gene            2304..2768
                     /gene="smpB"
                     /locus_tag="PKN90_04065"
     CDS             2304..2768
                     /gene="smpB"
                     /locus_tag="PKN90_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNZ85488.1"
                     /translation="MKTKQTAIRIKNKRATFDYEILERYTAGVVLVGTEIKALREGKA
                     NLTDSFCFFFNNELWVKNLSISPYFYGSYNNHNPRRDRKLLLTTKEFRKLERGTKEKG
                     CTLIPTLLFINEKGLAKLEIALAKGKKEYDKRDSIKEKEDKRVMDRAFKSKI"
BASE COUNT         1056 a          496 c          394 g          875 t
ORIGIN      
        1 acaaggagcc cgaaatattg aaaaaatcaa aagccatacc atatactacc attgatagaa
       61 ttatcatcca taagcgatca tcaggatttc caaaaccgaa caatgcaaaa cgtaataccc
      121 aagcaatcat actcattagc attacattct ttataccata gcgttttaaa aagaaaggta
      181 tagctaaaat aaacaatgtt tccgatattt gtgaaataga cattacaatg gttgaatacc
      241 taacgacaaa agagtcagca tattgttcaa tatttttaaa atcgtctaaa aacgtatctc
      301 catacatatt agtaagttga agtgctgccc ctaaaaacat cgaaaaaata aaaaagatag
      361 ccattttagg attagcaaat aatttaaacg catctaaacc aagcatttgt acaatggatg
      421 aagatttttc tttacttctt tgcgggatac atgcaggtaa cgtaaatgaa tagatggcta
      481 aacaaaaaga taaaactgct gaaatataaa attgtactgc tgttgctttg tttccagtta
      541 aatttaccac ccacatagca ataataaaac ctatcgtacc ccaaatacgt atgggtggaa
      601 atgctttttc aatatcaaga tttacttctt tcaaagcatt atatgcaata gaattactta
      661 atgaaatggt tggcatataa aaaaacatgg caagcaacat tacccaaaaa aatacaacag
      721 gtgaatttac aaacgcaaga gaaaaaatac ataacccact taacaagtgt agtaagccat
      781 ataatttttc tgcattaacc catctatcag caataatccc cattaatgta ggcattatta
      841 aagaagaaat acccaaagtg gaaaatactg ccccaaattg agccccatcc cattgttttg
      901 tatcaaacca ataaatagca atagtaataa gccatgcccc ccacacaaaa aattgcagaa
      961 aactcatcac tatcagtcta aatttaagat ttttcatatt tcttaatttg gatttttttc
     1021 ctcattatta gtagaacgtt ttattatctc ctctttacat aacaaagctt gtttataatc
     1081 ttcacgttca gcagctaatt gcatttcttg ttttaattcc tcatacgtca tatcagccaa
     1141 tttcttggtc ttaattgcta atcgttgttg cattgctact gtcaatactt cttcagagac
     1201 aaatatagga caaccacaac gaacagcaat agcgacccca tcagatggtc tgcaatcact
     1261 ctgcaaaaca tgtacaccca ttctataaac taatttcgaa aaaaaaatac catcttgaag
     1321 tttatcaata acaatacgtt cgattgcaac attaagattg ctcactaaat ttactattac
     1381 atcgtatatg agaggtcgtg gtactttttt ctgttcgagc tgaatggcaa tagattgtgc
     1441 ctcactatat cctatttcta tcatcaaata cttacttcct gcaacttctt ttaacaatag
     1501 tgtatgagtt tctgttttag aaattccaag actaacgcca aaaacatata actcaatcat
     1561 ttgcaagcat tttattttag gctgacaaag ttaagaaata aaaatcaaaa aagaaaaaag
     1621 aaaaatgcct tttatataag aaaaaacaaa aaatagggtt aattcatatt ttactaaaaa
     1681 aaatagtatt tttgtgtccc gtaacaacga aaaaaaatta gaacaatgta caaagaatta
     1741 attaaaacta ttgcattatt tatagctaaa cctgtaaaag cttgggaata cattaacaca
     1801 aaaaatccta ctgaaaataa tccactacat tcttatgcct accctttaat gggtttaggc
     1861 tcgttatctg cttttatagt agctctcgtt aataacggta gcaacgatgg atttcaaatt
     1921 gcactaaagg aagcctcact attatttatt tcaagtttta ttggcttgta tactgccgca
     1981 tttctaattc gtatttttta tgaaaaatat tgcaaagaac ctctttcttt tgatgttacc
     2041 attcaatttg ttgtatatag ctcttctgta atttttatta ttaacttgtt taatcaagtt
     2101 actagtagca ttttctttct acaaatattc tctttgtaca ctatatatat agtatgggaa
     2161 ggaagcattc attttttaaa aatagaagaa tcaaaaaggg ctagttttgt tcttttcagt
     2221 gctttttgta tgatcctttt accaatagca atcacggcat taatgaaatt ctttatgccc
     2281 ggtttacaat aagttaatca accatgaaaa ccaaacaaac tgccattcgg ataaaaaaca
     2341 aacgagctac gtttgattat gaaattttag aacgctacac agcaggcgtt gtacttgtag
     2401 gtacggaaat aaaagcttta cgagaaggaa aagccaattt aaccgattct ttttgtttct
     2461 tttttaataa tgaattatgg gttaaaaact tgtcaatttc tccctatttt tatggatcgt
     2521 acaacaacca taaccctcga agagaccgca aattattgct caccacaaaa gaattccgta
     2581 aactagaacg tggcacaaaa gaaaaaggtt gcacgctaat ccccacttta ttatttataa
     2641 atgaaaaagg attagctaaa ctagaaattg ctttagcaaa gggaaaaaaa gaatacgaca
     2701 aacgagatag tattaaggaa aaagaagata aacgtgttat ggacagagct tttaaatcga
     2761 agatataaaa aaagccttac gaaacgcaaa gcttttgtag cgggaacgag agttgaactc
     2821 g
//
LOCUS       DAOPUR010000185         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_37008_length_2819_cov_2.439219, whole genome shotgun sequence.
ACCESSION   DAOPUR010000185 DAOPUR010000000
VERSION     DAOPUR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37008_length_2819_cov_2.439219"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>235
                     /locus_tag="PKN90_04070"
     CDS             <1..>235
                     /locus_tag="PKN90_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85489.1"
                     /translation="FIILLLTQYATAQDTFKILGHFPNFPNSTYELKGYEGMQQKTLS
                     TAASKEEGKFTLNYPKEYKGVAQLWMNGAYQVLL"
     assembly_gap    236..245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <246..1320
                     /locus_tag="PKN90_04075"
     CDS             <246..1320
                     /locus_tag="PKN90_04075"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNZ85490.1"
                     /translation="NKEDITLHWEDLTNRDNLQTNSKEYEAFLGGMKTFQDSEAKLTG
                     LHYLIPLYAKDSLKQQVFIKELDTVAYAFPKYVKNIPDNLFVRQYLLTKGLIEQMPKT
                     VETYKWRAPNHIPEFMAIDFKALKNSGLYNEIISGYTNLVERFPLEEVYEILNPAIDK
                     VITELKDEPTLQQEIAQNWFTLLEQKSLFKAAEHLALRMLNQENCMLNDKSKDKFEQY
                     RALAIGKTAPNIVLNLKNQNDLRNLKNNYKLVVFGASWCPNCQTDYPSLVGKYKKLKE
                     KHDLEVIYISIDTDKKVFEEYYKETPFITFCDTKGWETQAAKDYHVFATPTYILLDKE
                     LKILSKIQSPEHLEAWLQANGKK"
     gene            1378..1878
                     /locus_tag="PKN90_04080"
     CDS             1378..1878
                     /locus_tag="PKN90_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85491.1"
                     /translation="MPFTNFDSRHFATTEKTAVNNAVTAIETALATKLANLTAEERQQ
                     YGSVNEQNKLIINKVKDFRDSQPALSSPDVDWTEFMSDYDTRAFIQATMQRLQSLIDG
                     LNNAKILHDWDNYQASLTDYDYAKYKASTQAVGYQTKVSELAQFFTGGNTTSAKNTNT
                     DNTTAE"
     gene            complement(2276..>2819)
                     /locus_tag="PKN90_04085"
     CDS             complement(2276..>2819)
                     /locus_tag="PKN90_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85492.1"
                     /translation="LFWMYEMVTEWITSDDENEEKQIHLSEIKRAEQIGSFMEQKIYN
                     HHNLTRFKDHLKNFKAKDGFDNDCFMLARKVFSLYEQYPNATIYQNLQQNTEEDYEYE
                     IETTVSIDKYISFCAKGKGLLFQTLFEAVNSEFQECTTMEEPIVIKTFDGSNITNNNL
                     DFENRLFPLIEELIYILNNF"
BASE COUNT         1001 a          491 c          474 g          843 t
ORIGIN      
        1 tttattatcc tattgctaac tcaatatgca accgctcaag atacttttaa aatacttggg
       61 catttcccca actttcctaa tagcacttat gaactcaaag gctatgaggg tatgcaacaa
      121 aaaacgctat ctactgcagc aagcaaagaa gagggcaagt ttacacttaa ttatccaaaa
      181 gagtataaag gtgtagccca actgtggatg aatggggcgt atcaagtatt gttatnnnnn
      241 nnnnntaaca aggaagacat taccctccat tgggaagacc ttaccaatag agataatcta
      301 caaaccaaca gtaaagaata cgaagctttt ttgggtggta tgaaaacctt tcaagacagc
      361 gaggcaaagt tgacaggttt gcattatctc attccgctat atgccaaaga cagccttaag
      421 caacaagtgt ttataaaaga gttagacaca gtcgcctatg cttttccaaa atatgtaaaa
      481 aacatacccg ataacttatt tgtgcgtcaa tacctgctta ccaaaggctt gatagagcaa
      541 atgcccaaaa cagtagaaac ctacaaatgg cgagctccca atcacattcc tgagtttatg
      601 gcaatagatt ttaaagcctt aaaaaattca ggtttatata acgagataat tagtggttac
      661 acaaacttag tagagcgttt tccgttagaa gaggtttatg aaattttaaa cccagccata
      721 gacaaagtca tcaccgaact gaaagacgaa cctaccttac agcaagaaat agcccaaaac
      781 tggtttacgc ttttagaaca aaaaagcttg tttaaagcag ccgaacattt ggctctaagg
      841 atgctgaatc aagaaaactg tatgttgaat gacaagagta aggataagtt tgaacaatat
      901 agagcattag ccataggcaa aaccgctcct aatattgttt taaatttaaa aaatcaaaat
      961 gatttaagaa acctaaaaaa caactacaaa ttagtagttt ttggagctag ttggtgtcct
     1021 aattgtcaaa ctgattatcc atctttagta ggaaaatata aaaaactaaa agagaaacat
     1081 gatttagaag taatttatat ttctattgat acagataaaa aagtgtttga agagtattat
     1141 aaagagacac catttatcac tttttgtgat accaaaggtt gggaaaccca agccgcaaaa
     1201 gactatcatg tatttgctac accaacctat attttattag acaaagagtt aaagatttta
     1261 tctaaaatac aatcgccaga gcatttagaa gcttggctac aagctaatgg aaagaaatag
     1321 aagtactata atttataact attaattcat aattcataat tcataatttt tatacctatg
     1381 ccattcacca attttgacag cagacatttt gctacaacag aaaaaaccgc agtaaacaat
     1441 gccgtaactg caatagaaac cgctttagct accaagttag ctaacttaac tgccgaggag
     1501 cgacaacagt atggtagcgt aaacgagcaa aacaagctca tcatcaataa agtaaaagac
     1561 tttagagaca gtcaaccagc acttagcagc ccagatgtag attggacaga gttcatgagc
     1621 gattatgata ctcgtgcatt tattcaagct actatgcaac gtttacaaag tttgatagat
     1681 ggtttaaata atgcaaaaat actccacgat tgggacaact accaagcctc acttacagat
     1741 tatgattatg ccaaatacaa agcaagcaca caagccgttg gttatcaaac caaagtaagc
     1801 gagttagcac agttttttac aggaggcaac accacaagcg caaaaaatac aaatacagat
     1861 aatacaacag cagagtagtt tatattaaaa aaccagcata tttgcaaaat taactatttg
     1921 gggtagttaa tttgatttat tttgtttaac catcacaagc aattgtggtg gtttttttat
     1981 gcaaaaccgt ggtcaaccac gttcctaaga ccgttttaca tgttgtataa cgtggtaaat
     2041 ggtgtttaca acctataaat acacgttgac taacctataa aatgcagttt ttaacgcggt
     2101 taaccacgtt acctaacgtg gtaaatgggg taaataacgt ggttaaccac gtcaaccaac
     2161 gaggtaaatg tatttatgta cgtggtcaac cacgtagtat aacggggtaa aagtagtttg
     2221 gaggcgtggt agagtgttcc aaaatcttgg ataatatctt tcatcttcta tttatttaaa
     2281 agttatttaa aatgtaaatg agttcttcaa tcaatggaaa caatcggttt tcaaaatcga
     2341 gattgttatt tgtaatgttg cttccatcaa atgtttttat aactattggt tcttccattg
     2401 tggtacattc ttgaaactca gaatttactg cctcaaaaag cgtttggaat agtaatcctt
     2461 ttcctttggc acaaaaagaa atgtatttat ctattgatac ggttgtctct atttcgtatt
     2521 cataatcttc ttctgtattt tgttgcagat tttggtaaat ggtagcattt ggatattgct
     2581 cataaagaga aaatactttt ctcgccaaca taaaacaatc attgtcaaaa ccgtctttgg
     2641 ctttaaagtt ttttaaatgg tctttaaatc gagttaaatt atgatgattg tatatctttt
     2701 gctccataaa actacctatt tgctctgctc gtttgatttc tgataaatga atctgctttt
     2761 cttcgttttc atcatcagaa gtaatccatt ccgttaccat ttcgtacatc caaaacaag
//
LOCUS       DAOPUR010000186         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_37123_length_2811_cov_4.232946, whole genome shotgun sequence.
ACCESSION   DAOPUR010000186 DAOPUR010000000
VERSION     DAOPUR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37123_length_2811_cov_4.232946"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PKN90_04090"
     CDS             <1..498
                     /locus_tag="PKN90_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNZ85493.1"
                     /translation="IIYGISVFGLSDRLSISRSEAKELIDGYFETYPGVKSYMEASIA
                     KAKEQGYVETLLGRKRFLPDINSSNSFVRGFAERNAINAPIQGSAADIIKIAMIRITQ
                     RFEQENIQSQMTMQVHDELNFSVLKSELEIVKRIVIEEMEHAISLRVPLVADCGVGDN
                     WLEAH"
     gene            828..1289
                     /gene="tnpA"
                     /locus_tag="PKN90_04095"
     CDS             828..1289
                     /gene="tnpA"
                     /locus_tag="PKN90_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNZ85494.1"
                     /translation="MANTYTQLYIHIVFTVKGRQNLIQKQWREELHKYICGIVNRQNE
                     KVYAIGGVADHVHILISIKPNIAISDLVREIKANSSKWVNEKRFVLGKFEWQEGFGAF
                     SYAQSQLNAVINYINTQEHHHKNKTFKEEYLDLLSKFSIEYEEKYLFDWIE"
     gene            complement(1737..2153)
                     /gene="tsaE"
                     /locus_tag="PKN90_04100"
     CDS             complement(1737..2153)
                     /gene="tsaE"
                     /locus_tag="PKN90_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNZ85495.1"
                     /translation="MPQLQIPSLQELPVVATTFIRLLPKDTRIIAFEAGMGVGKTTFI
                     KAICQVLGVQDVVNSPTFSIVNEYACSNGESVYHFDFYRLQSPQEALDFGVYDYFESG
                     NWCFMEWAENIAPLLPTETLFVTLMENPDGSRDIRF"
     gene            complement(2167..>2811)
                     /locus_tag="PKN90_04105"
     CDS             complement(2167..>2811)
                     /locus_tag="PKN90_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445284.1"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNZ85496.1"
                     /translation="STLGGREVWFDKDVMRLNYDGRGVSLGEFDSDDSILVLSKDGCF
                     YTTNFELSNHFEDNVLLLEKFDEDKVWSAVLYDAEQQFYYVKRFTLEQTNKKTSFIGD
                     NPESRLLLMSTQVYPRLQVLFGGKDAEREPQEIDVEEFIGVKSFKAKGKRLTTFEIKD
                     IVELEPTRFPEVVDEEPETADEMDINDGNEKVDDQEVIDKKKIIEDITGQMSLF"
BASE COUNT          991 a          553 c          467 g          800 t
ORIGIN      
        1 atcatttacg gcatctcggt atttggactc agcgaccgac tcagtatctc acgcagcgaa
       61 gccaaagaac tcattgatgg gtattttgaa acatacccag gagtaaaatc atacatggaa
      121 gcgagcattg cgaaagccaa agaacaagga tatgtagaaa cgcttttagg tcgtaaacgg
      181 tttttacctg atattaattc ctccaatagc tttgtacgtg gctttgccga acgaaacgct
      241 atcaatgcac ccattcaagg ctctgctgcc gatataatta aaatagccat gatacgtatt
      301 acacaacgat ttgaacaaga aaatatacaa tcacaaatga ccatgcaggt gcatgatgag
      361 ctgaacttta gcgtgttaaa aagtgaactg gaaatcgtaa aacgtatcgt aattgaagag
      421 atggaacatg ctatttcact acgagtacct ttagtggctg attgtggtgt aggagataat
      481 tggttagagg cacattaata attttctccc caccaaaact ccagcgaggc gatataataa
      541 taacataaag caataaaagc aagttttatc tcgctcctac ggagcttcgt tattcatttg
      601 tctattaccc tattatgatg gtactcctac ggagcacgaa acacatttga taagaaatcg
      661 tttaaggtat aaaaatacaa gaaaatggaa aatcaaatag aagctaacta attccttcaa
      721 actaagcccc agaggggcga tataataata gaataaaaac acagcaaaca aagtaagctc
      781 cataggagcg acataaaaaa cccatcagag acattaataa aatttttatg gcaaatacgt
      841 atacgcaact atatattcac atcgttttta cagtaaaagg caggcaaaat ctcatccaga
      901 aacaatggag agaagaatta cacaaataca tttgtggcat tgtaaatcgc caaaacgaga
      961 aagtatatgc tattggagga gtagctgacc acgtacatat actcatcagt ataaaaccga
     1021 acatagcaat ttcagattta gttcgtgaga ttaaggcaaa ttcctctaaa tgggtaaacg
     1081 aaaagcgttt tgtgttgggg aaattcgaat ggcaagaagg atttggagct ttctcatacg
     1141 cacaatctca attaaatgcg gttataaatt atattaatac gcaagaacat catcataaaa
     1201 ataaaacgtt taaagaagaa tacctagacc ttttaagtaa gtttagtatt gaatatgagg
     1261 aaaaatattt atttgattgg attgaatagt tttgaaatac gtatcgtgtc gcaagcatta
     1321 tgtcgctcct acggagcttg tgttattcat ttatccatta cctattatga tggtactcct
     1381 acggagcacg aaatacctta atatgaaaga acaatcaaaa agaattcctc cccaaaaagc
     1441 ttcagcgagc tatatcatga tacaacaaag caataaaagc aagtttttat ctcgctccta
     1501 cgaatcttgt gttattcatt tgtctattac cctattatga tggtactcct acggagcacg
     1561 aaacacattt gataagaaat cgtttaaggt ataaaaatac aagaaaatgg aaaatcaaat
     1621 agaagctaac taattccttc aaactaagcc ccagaggggc gatataataa tagaataaaa
     1681 acacagcaaa caaagtaagc tccataggag cgacataaaa acccccaata catttattaa
     1741 aaccgaatat ccctgcttcc atcgggattt tccattaggg ttacgaaaag agtttccgtt
     1801 ggtaataagg gagcaatatt ttctgcccat tccatgaaac accaattgcc actttcaaaa
     1861 taatcgtaca ctccaaaatc caatgcctct tggggagatt gtaatcggta aaaatcaaaa
     1921 tgatatacag attcgccatt gctacaagcg tattcattca cgatagaaaa cgtagggcta
     1981 ttgacaacat cttgcacacc caacacctga caaatagctt tgataaaggt agttttacct
     2041 acccccatac ctgcttcaaa agctatgata cgggtatctt tgggaagtaa gcgtataaaa
     2101 gtagtagcta ctactggaag ttcctgcaac gatggtattt gaagttgtgg cataaaggag
     2161 atttaattaa aacaaactca tctgaccagt aatatcttcg attattttct ttttgtcaat
     2221 tacttcttga tcatctactt tttcatttcc atcatttata tccatttcat cagcagtttc
     2281 aggctcttca tctactactt ctggaaaacg tgttggctcc aactctacaa tatctttgat
     2341 ttcaaatgta gttaaacgtt tccctttggc tttaaaactt ttcacgccaa tgaactcttc
     2401 tacatcaatt tcttgcggtt cacgctcggc atctttacca ccaaacaata cttgcaaacg
     2461 tggatatacc tgcgtagaca tcagcaacaa acgtgattcg ggattatctc caataaatga
     2521 tgtttttttg ttggtttgtt ctaaagtaaa acgttttaca taataaaact gttgctcagc
     2581 atcgtacagt acagctgacc acaccttatc ttcatcaaat ttttcgagca acaagacatt
     2641 atcttcaaaa tgattactca attcaaaatt tgtagtataa aaacaaccgt ccttggataa
     2701 taccaaaatc gaatcatcac tatcaaattc gccaagcgat acgcctcttc catcataatt
     2761 taatcgcatc acatctttat caaaccacac ttcgcgaccc cctaaggtag a
//
LOCUS       DAOPUR010000187         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_37483_length_2791_cov_3.997442, whole genome shotgun sequence.
ACCESSION   DAOPUR010000187 DAOPUR010000000
VERSION     DAOPUR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37483_length_2791_cov_3.997442"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PKN90_04110"
     CDS             complement(<1..1188)
                     /locus_tag="PKN90_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNZ85497.1"
                     /translation="MRKLFFCFLFLLISIFSFYAESYSAYYLSAEGLSREHLKTALQR
                     RASKHQFLLYDELWNYFQQTDALDNNRVLDRYSSTEYVFSQSSQMDREHAFPKSWWGG
                     ATFYPVYSDLHHLYPSDRSANIAKSNNPLGKVSTNPSFSNGVSQVGPSVISGYSGTVF
                     EPADEYKGDFARAYFYVVTAYEQLFGIWDSPMLDNNTYPVFKTWAIDLLLEWHTNDPV
                     SQLEIDRNEQVYLVQQNRNPFIDYPQLVDYIWGNKQQDVFYTSSVITEPTIVSPWQDD
                     TIQFPAIFEDDEIEETVYIRGLNLSEGLSLSITDDANSYFSLAETTVSKTNAEQPNAY
                     PIKIKFSPTEVKEATAILTISGGGATPVSVVLSGTSLNRAVAKPEAQPISNVENNAAT
                     LSWT"
     gene            complement(1213..2373)
                     /locus_tag="PKN90_04115"
     CDS             complement(1213..2373)
                     /locus_tag="PKN90_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNZ85498.1"
                     /translation="MKFFITYITFFCVFFYVHASEFDSDVYYQEAEGKQQYELKTALH
                     KIIRGHTRVSYTPGIWQAYEKTDLRQNGTVWCIYTSCPFNYGVHPDYVEGYQCGSTGM
                     GYTECFCYSREHSFPKSFWGGGTALSDTVYTDVFHIYPASGRVNSVLRNDNVYANVGT
                     IIPKNNDDNPTSNGSKLGYSSVVGYDGQAFEIADEYKGDIARTYFYIATRYQHLIPQW
                     YTTSTGTKLNNARFILASNTSSVFQSWYINMLLAWHRQDPVSAKEIDRNNAVYTHVQN
                     NRNPFIDHPELVEYIWGNKQAEKWRQPTSIKTTTENLFSIYPASDTNFFFCNIKSDAD
                     IRFVIQDVSGKIIRRGVFSPPYQFFIKDLHKGIYIMHVKENGMLSKTTFFVK"
     gene            complement(2390..>2791)
                     /locus_tag="PKN90_04120"
     CDS             complement(2390..>2791)
                     /locus_tag="PKN90_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ85499.1"
                     /translation="HRQDPVSPKEVTRNNAIYAHQDNRNPFIDYPELAEYIWGTKKSL
                     SWNPTSLDEDPNFQYFVYPNPVSDILYVHVDTTKNVQYVLYSVDKKVLASGVFDSNWS
                     IDVRALQSGMYVLQIYVEGVSYSYKVLVNSN"
BASE COUNT          900 a          536 c          473 g          882 t
ORIGIN      
        1 cgtccatgat aaggtagccg cattgttttc aacatttgat atgggctgtg cttctggttt
       61 ggctactgcc ctatttaagg atgtacctga taaaacgaca gagacaggcg tggctcctcc
      121 tccagaaata gtaagaatag cggtagcctc tttaacctcg gtaggcgaaa atttaatctt
      181 aataggatat gcatttggtt gttctgcatt tgttttagaa acagtagttt cagccaaaga
      241 aaaataacta ttcgcatcat ctgtgatgga aagagataag ccttctgata aattcaaacc
      301 tcttatatag accgtttcct ctatctcatc atcttcaaaa atagcaggaa attgtatcgt
      361 atcgtcctgc cacggagaaa ctatggtggg ttctgttatt acactagaag tgtagaatac
      421 atcttgttgt ttatttcccc aaatataatc tactagttga ggataatcga taaatggatt
      481 tctattctgt tgaacaagat atacttgttc gttcctatct atttcaagct gcgaaactgg
      541 gtcattagta tgccactcta acaacaaatc gatagcccaa gtcttaaaaa caggataggt
      601 attattgtct aacatgggcg aatcccatat gccaaacaac tgctcatagg ctgttacaac
      661 ataaaaataa gcccgagcaa aatcgccttt gtattcgtct gcaggctcaa aaacggttcc
      721 tgaatatccc gatattacag agggtccaac ttgacttacc ccattagaaa atgaaggatt
      781 agtagagact ttccctaatg gattattgct tttggctata ttagcactcc tatcgctagg
      841 atagaggtga tgcaaatctg aatacactgg ataaaaagtg gctccacccc accagctttt
      901 tggaaaagca tgttccctat ccatttgtga tgattgggaa aaaacatatt cggtagatga
      961 ataacggtct aacacacgat tgttatccaa tgcatctgtt tgctgaaaat aattccaaag
     1021 ctcatcatat aacaaaaatt gatgcttact cgcccttcgt tgcaatgcag tttttaaatg
     1081 ttctctactt aatccttcag ctgataaata ataagcacta tatgattcag cataaaaaga
     1141 aaagatacta atcaaaagaa ataagaagca aaaaaataat tttctcataa tgctgtgtac
     1201 tttaaaaaaa tattatttca caaagaaggt agttttactt aacattccgt tttcttttac
     1261 gtgcataata taaatacctt tatgcaaatc tttaataaaa aactgatatg gaggagaaaa
     1321 cacgcctcta cgaattattt ttcctgacac atcttgaata acaaacctaa tatctgcatc
     1381 cgacttaata ttacagaaaa aaaaattagt gtcgcttgct ggataaatac tgaataaatt
     1441 ttctgttgtt gtttttattg atgttggctg acgccatttt tcagcttgtt tatttcccca
     1501 aatatattcg accaattcag ggtgatcaat aaaaggatta cggttatttt gtacgtgtgt
     1561 ataaacggca ttatttctat ctatttcttt tgcagatacg gggtcttgtc tatgccacgc
     1621 aagtagcata ttgatatacc atgactgaaa aacagaactc gtatttgatg ctaatataaa
     1681 acgggcatta ttcagttttg ttcccgtcga agtggtatac cattgcggta taagatgttg
     1741 ataacgagta gctatataaa aataagtacg tgcgatgtca cccttgtatt catctgctat
     1801 ttcaaaagcc tgaccatcgt atcctacaac agaagaatag cctaatttag aaccgtttga
     1861 agtaggattg tcgtcattat tttttggaat tattgttcca acattcgcat atacattatc
     1921 atttcggagc acgctattta ccctcccact cgcaggataa atatgaaaca catcggtata
     1981 tacagtatca gataatgctg ttcctccgcc ccaaaaactt ttaggaaaag aatgttctct
     2041 gctataacaa aaacactccg tataccccat tcccgtagaa ccacattgat agccctctac
     2101 ataatcagga tggacgccat aattaaatgg gcacgaagta tatatgcacc aaacggttcc
     2161 attttgtctc aaatctgttt tttcataggc ttgccaaata cctggtgtat agcttaccct
     2221 agtatgcccg cgtattattt tgtgtaatgc tgtttttagt tcatattgtt gttttccctc
     2281 tgcttcttgg taatacacat cactatcaaa ctctgatgca tgtacataaa agaaaacaca
     2341 aaaaaaagtt atgtatgtaa taaaaaactt cattgcatgt gccaaaaaat taattgctat
     2401 ttacaagaac tttatacgaa tacgacacac cctctacata tatctgtaac acatacatac
     2461 ccgactgtaa agcacgaaca tcaatactcc agttagaatc aaacactccg ctagcaagca
     2521 cttttttatc tacagaatac aatacgtatt gcacattttt agtagtgtca acgtgtacat
     2581 agagtatatc agaaacagga ttaggataca caaaatactg aaaatttggg tcttcgtcta
     2641 aggaggtagg attccatgat aatgactttt ttgttcccca aatatactca gctaattcag
     2701 gataatcgat aaacggattg cggttatctt ggtgtgcata tatcgcatta ttacgggtta
     2761 cctctttagg cgaaacgggg tcttgtcggt g
//
LOCUS       DAOPUR010000188         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_37665_length_2781_cov_3.577036, whole genome shotgun sequence.
ACCESSION   DAOPUR010000188 DAOPUR010000000
VERSION     DAOPUR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37665_length_2781_cov_3.577036"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKN90_04125"
     CDS             complement(<1..461)
                     /locus_tag="PKN90_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ85500.1"
                     /translation="MAWVNFHSHTSYCDGIAQPEAYVLQAIELNMPAYGFSGHAPMPY
                     STNWAIKQDQLTSYINEIQRLKEVYTSQIDIYCAVEADYIPQISGPHLFTNYPFDYII
                     GAIHFVGYFADGKPFSVAGKALDFDKGLKEIYGNDIKRLVKAYFSLNCELML"
     gene            complement(541..903)
                     /locus_tag="PKN90_04130"
     CDS             complement(541..903)
                     /locus_tag="PKN90_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ85501.1"
                     /translation="MKIAVPVKSTNQIDAHFGHCEGYKVFTIENNNIIAEENVPSPKG
                     CGCKSDIASVLHQKGVTVMLAGGIGDGAVQVLSSNGIQVVRNCKGDANAVLVEYLQGN
                     IVDGGVNCSHDHNHQCSH"
     gene            complement(922..1773)
                     /locus_tag="PKN90_04135"
     CDS             complement(922..1773)
                     /locus_tag="PKN90_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ85502.1"
                     /translation="MSLFVKIIVDNHSQQDNFLTEHGICVYVETPTHKCLVDVGASDK
                     FIRNAEQMGIDIAQIDYLFLSHGHVDHVGGLPFFLKKNAKAKIIFSDKLFTQQYFSKR
                     HSTRSISLAYTFTEEELNRFMPIQQTAHIEADIKVIAQIQQNYPLPQGNRFLYTQAFD
                     DNEFVLDDFSHEIVVCFGSTSLLVCTGCAHNGLLNMLHTVQNQTSLPIHTVLGGFHLL
                     DADGTYAYETPKEITDIALTLKQTYPSTQFFTGHCTGANSIEILQQTLSSQLEAFYCG
                     WQKQLNY"
     gene            complement(1770..2180)
                     /locus_tag="PKN90_04140"
     CDS             complement(1770..2180)
                     /locus_tag="PKN90_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNZ85503.1"
                     /translation="MPRPQQHRTLLTPPKMKGFKPFGIPTKSLSSVVLLYDEYESIRL
                     LDYEGLTQEQAAERMGVSRPTLTRIYEKARQTMAQAMVEGKMILIEGGNVSFNSQWYR
                     CKKCYKLIQGKENHLHCDNCPCYGDDELEKIGQE"
     gene            complement(2183..2542)
                     /locus_tag="PKN90_04145"
     CDS             complement(2183..2542)
                     /locus_tag="PKN90_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNZ85504.1"
                     /translation="MKKTLVIGASENPERYAYKAITALVAHGHEVKALGLKKGNVAGI
                     EFDTEQLPYQGIDTVSLYVGPQNQPSYYEFIEKLQPKRVLFNPGTENSEFQARLIAKG
                     IEVEEACTLVLLSTNQY"
BASE COUNT          849 a          540 c          485 g          907 t
ORIGIN      
        1 agcattagtt cacaattgag cgaaaaatag gcttttacca agcgttttat atcgttgcca
       61 taaatttctt ttagtccttt atcaaaatct aaggctttgc cagctacgct aaaaggtttt
      121 ccatcagcaa aataaccaac aaaatggata gctccaatga tataatcaaa gggataattt
      181 gtaaacagat gcggacctga tatttgtgga atataatcag cctctactgc acaataaata
      241 tctatttggg acgtatatac ttctttaagt cgctgtattt cattaatata cgatgttaat
      301 tggtcttgtt ttatcgccca atttgtacta tatggcatag gagcatgacc agaaaatcca
      361 taggcaggca tgtttagctc aatagcttgc aacacatacg cctctggctg tgctatccca
      421 tcgcaataag aagtgtgtga atgaaaattg acccaagcca tactaaaact attagctaca
      481 aagatacggt aattttcact ttacaaaaag tgaacacata tcaaaataaa aaatatattc
      541 ttaatgagag cattgatggt tgtgatcgtg gctacaattc actccaccat ccacaatgtt
      601 gccttgtaaa tactctacca aaacagcgtt ggcatctccc ttgcaattac gcaccacttg
      661 tataccattg cttgataaca cctgtacagc accatcgcca ataccaccgg ctagcattac
      721 tgtaacgcct ttttggtgaa gaacactggc aatgtccgat ttgcatccac agcctttggg
      781 ggaaggaaca ttttcttcag caataatatt gttgttctca atagtaaata ctttgtagcc
      841 ttcgcaatga ccaaaatgag catcaatttg attagttgat tttacaggaa cggcaatttt
      901 catacgatta tagttttaaa attagtaatt tagttgtttt tgccaaccac aataaaaggc
      961 ttccaactga ctcgaaagtg tttgttggag tatttcaata gagttggctc ccgtacaatg
     1021 cccagtaaaa aactgggtgg agggatatgt ttgttttaat gtaagagcaa tatcggttat
     1081 ttcttttggc gtttcgtaag catacgttcc atcggcatct aacagatgaa atcctcctaa
     1141 cacagtatgt atgggcaacg atgtttgatt ttgtactgta tgcaacatat tcagcaaacc
     1201 attgtgggca catcctgtac acacaagcaa cgaagttgac ccaaaacaca ccactatctc
     1261 gtgcgagaaa tcgtccagta caaattcatt atcatcaaag gcttgagtat ataaaaaacg
     1321 attcccttgt ggcaatgggt agttctgttg tatctgtgca attactttta tatccgcctc
     1381 aatgtgagca gtttgttgaa ttggcataaa acgattcagc tcctcttcgg taaaagtata
     1441 tgctagacta atagagcgtg tggaatgacg tttagaaaaa tattgttgcg taaagagctt
     1501 gtcagaaaag attatctttg ctttcgcgtt ctttttcagg aaaaacggaa gaccaccaac
     1561 atgatcgaca tgtccatgag aaagaaacag ataatcaatt tgagcgatat caatgcccat
     1621 ttgttcggca ttgcgaataa acttatcaga ggcaccaaca tcgactaaac atttgtgtgt
     1681 aggcgtttct acatacacac agatgccatg ctccgtaaga aagttatcct gttggctatg
     1741 gttatccact attattttta caaacagact cattcttgcc caattttttc taattcgtcg
     1801 tcgccataac atggacaatt atcgcaatgc aaatgatttt ctttgccttg tatgagtttg
     1861 tagcattttt tacatcgata ccattgcgaa ttaaaagaaa cattccctcc ctcaatcaaa
     1921 atcatcttac cctctaccat agcttgtgcc atagtttggc gagctttttc gtaaatacgc
     1981 gttaatgtag gtctagaaac ccccattctt tctgccgctt gttcttgggt gagtccttcg
     2041 taatccaaca aacgaataga ttcgtattcg tcgtacaaca acaccacaga agacaatgat
     2101 ttagtgggga taccaaaggg tttaaacccc ttcatttttg gcggggtcaa taaggttcta
     2161 tgttgttgtg gtcgtggcat acttaatact gattggttga aagcaatacc aatgtacaag
     2221 cttcttctac ttcaattcct ttggctatta atcgtgcttg aaattcggaa ttttcggtac
     2281 ctggattaaa caatacgcgt tttggttgaa gtttttcaat aaattcgtaa taacttggtt
     2341 ggttttgtgg accaacatac aagcttactg tatctatgcc ttgataaggt agctgctcgg
     2401 tatcgaactc aataccagct acgtttcctt tttttagtcc taaggctttt acctcatgac
     2461 catgtgctac taaagccgta atggctttgt atgcgtatct ttctggattt tctgatgctc
     2521 caattacgag tgtttttttc atattactat ttgtagtttt gttacacaat tgttttaatt
     2581 gaactacaaa tataatgaac atttgttcat tataaaaata aaatagtaaa atttaacgat
     2641 tttttgtagt acaacaaaaa tttacccttt atacactaat taaatttact tttccatatt
     2701 taattagtgt ataaaggact atacgataga aaagaaccta cattttctct ttctgcgcat
     2761 tacgtatcat aaaatgcaag c
//
LOCUS       DAOPUR010000189         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_37671_length_2781_cov_2.653705, whole genome shotgun sequence.
ACCESSION   DAOPUR010000189 DAOPUR010000000
VERSION     DAOPUR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37671_length_2781_cov_2.653705"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKN90_04150"
     CDS             complement(<1..280)
                     /locus_tag="PKN90_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HNZ85505.1"
                     /translation="MEQLYVNSTYVSLFFSILCCLVLLLCSAFVSASEVAFFSLLPKD
                     IEELTASSDRKDTCILELKSNSDRLLATILLANNLVNIAIIVLSTSILF"
     gene            497..>2781
                     /gene="pheT"
                     /locus_tag="PKN90_04155"
     CDS             497..>2781
                     /gene="pheT"
                     /locus_tag="PKN90_04155"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNZ85506.1"
                     /translation="MNISYNWLKQYININLPVHEVSHLLTSLGLEVGSVEEVETIKGS
                     LEGLVVGEVIESSKHPDADRLHVAQVRIQPTEEPIQIVCGAPNCRAGIKVVVATVGTK
                     LYSGEEVFTIKRSKIRGVESNGMLCAEDEIGIGTSHDGIIEIASNIEVGTPVKELYNV
                     DTDTIFEVDITPNRVDAASHIGVARDLAAALRLKDSTIQLQKPSVESFVVDNKEYTIN
                     VEVTNPTLCPRYCGVTINNIQVKESPEWLKNRLLMIGMRPINNVVDITNFVLHETGQP
                     LHAFDGDKITGNKVRVTTLANNTPFVTLDEVERKLSNEDLMICNANEGMCIAGIFGGL
                     DSGVSNTTTKLFLESAYFNPVSIRKTARRHGLNTDSSFRFERGCDPNNTLYALKRAAL
                     LIKEVAGGEISSDIVDIYPSPIQATEVKLTYAKINSLIGKVIDKEIIRTILTGLEIDI
                     VNENNDVLTLSVPAYRVDVQRDVDVIEDILRVYGYNNIIPSDELKSSITYSQKPDSHK
                     LQNTLAEQLTGAGFNEILNNSLTKIAYYKELNSFPENEAVKILNPLSADLSVLRQTLL
                     FGGLESITYNSNRRNPNLKFYEFGNCYHYDDAKKNNEEPLKAYSEELHLGLWITGNKT
                     KQSWTDTERKVSFYDLKGYVETILQRLGIKNESISVSDYSNDLLPNALEIVSKNKMPL
                     VEIGKVSNKVLKQFDIDTDVFYADFNWENVLKVIVKQKVRYQEIPKFQEVKRDLALVL
                     DKHIRFQDIEQIAFATEKNLL"
BASE COUNT         1035 a          445 c          499 g          802 t
ORIGIN      
        1 aaaaaagaat actagtagac aatactataa tagcaatatt taccaaatta ttagcaagca
       61 aaatagttgc caataagcga tctgaattgc tttttaattc taaaatacac gtatcttttc
      121 ggtcgctact agctgttaat tcttctatat cctttggtaa tagagagaaa aatgctacct
      181 cagaagcaga tacaaaagcc gaacacaata ataaaactaa gcaacataaa atcgaaaaaa
      241 acaaggaaac gtatgtacta ttaacataca attgctccat aaaaagacgt atcgattcaa
      301 aaatatccaa tgtattacaa tttaaataaa acactacgta attagtaaaa cggaaaaata
      361 cgaagacaaa gatataaact tttaaagaaa atagtgaaat gaaatgatga agaataaatt
      421 ttttaaggga ataatttttt gtaatagcag atgtttgtta aatttgcact cttaaaatta
      481 ttttaataca aaaaaaatga atatttctta caattggctc aaacaataca ttaacatcaa
      541 tcttcctgta catgaagtat ctcacctact tacttctctt ggtttagaag taggctcagt
      601 ggaagaagtg gaaacaatca aaggaagttt agagggattg gtggtaggtg aagtgattga
      661 gagtagcaag catcctgatg ccgaccgttt gcatgtagct caagtacgta ttcaacctac
      721 tgaagaacca atacaaattg tatgtggagc tcctaattgt cgtgctggca taaaagtagt
      781 agtagctaca gtaggtacga aactgtattc tggagaagaa gtctttacca ttaaacgctc
      841 taaaatacgt ggagtagaat caaatggtat gttgtgtgcc gaagacgaaa ttggcattgg
      901 cactagccac gatggcatta ttgaaatagc ttcaaatatt gaagttggaa cgcctgttaa
      961 ggaactttac aatgttgaca ctgacactat ttttgaagta gatattactc ccaaccgtgt
     1021 agatgcagca tctcatattg gtgtagcacg cgatttggct gctgctttac gattaaaaga
     1081 ctctactata cagttgcaaa aaccctctgt agaaagtttt gttgttgata ataaggagta
     1141 taccatcaac gtagaggtaa caaaccctac cctttgtcca cgctattgtg gggtaaccat
     1201 taataatatt caagtaaaag aatcgcccga atggctcaaa aatagactac taatgattgg
     1261 tatgcgaccc ataaacaatg tagtggatat tacaaacttt gtgttgcacg aaacaggaca
     1321 acctttgcac gcatttgacg gagataaaat tactggaaat aaagttaggg ttactacctt
     1381 agcaaataac accccttttg ttaccctcga tgaggtagaa cgaaaattat ccaacgaaga
     1441 tttgatgatt tgcaacgcca acgaaggcat gtgtatagca ggtattttcg gaggattaga
     1501 ctctggagta agcaatacta caactaaatt atttttagaa agtgcttatt ttaatccagt
     1561 aagtatacgc aaaacagctc gtagacatgg actcaacaca gactcttctt ttcgttttga
     1621 aagaggttgc gacccaaaca acaccttata tgcactaaaa cgtgccgcat tactcattaa
     1681 agaagtagct ggtggcgaaa tttcttctga tattgttgat atatatccct ctcctattca
     1741 agcgacagaa gtaaagctta catacgccaa aattaattca ttaataggaa aagtaattga
     1801 caaagaaata attagaacaa tattaactgg cttagaaata gacatagtta acgagaataa
     1861 tgatgtactt acgctgtctg ttcctgccta cagagtagat gtacaacgtg atgtagatgt
     1921 gattgaagat attttgcgtg tatatggtta caacaatatt attccgtctg acgaattaaa
     1981 atcgtccatt acgtattctc aaaaacctga ttcacataaa ttacaaaaca cactagcaga
     2041 acagcttaca ggagctggtt ttaacgagat tttaaataat tcgcttacaa aaattgctta
     2101 ttacaaagaa ctaaactctt ttccagaaaa tgaagcagtg aaaatactaa atcctttaag
     2161 tgctgattta agtgttttgc gacaaacctt gctgtttggt ggtttagaaa gcattacgta
     2221 taacagtaac agaagaaatc ctaatttgaa attttacgaa tttggaaact gctaccacta
     2281 cgatgatgct aaaaaaaata acgaagaacc cctaaaagca tattcagaag aattgcattt
     2341 gggcttatgg ataacaggaa acaaaacgaa acaaagttgg acagatactg aacgtaaagt
     2401 gagtttttat gatttaaaag ggtatgtaga aacaatattg caacgattag gcattaaaaa
     2461 tgaatctatt agcgtttctg attacagcaa tgacctattg cctaatgctc tcgaaattgt
     2521 aagtaaaaat aaaatgccat tggtggaaat cggaaaggtt tctaataaag tgttaaaaca
     2581 atttgacatt gacacagatg tcttttatgc agactttaat tgggaaaatg ttctgaaagt
     2641 tattgtaaaa caaaaagttc gttaccaaga aatacctaag tttcaagaag tgaaacgaga
     2701 ccttgcatta gtactcgaca aacatattcg ttttcaagat attgagcaaa tagcatttgc
     2761 tacagagaag aatttgttga a
//
LOCUS       DAOPUR010000190         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_38812_length_2725_cov_3.062547, whole genome shotgun sequence.
ACCESSION   DAOPUR010000190 DAOPUR010000000
VERSION     DAOPUR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38812_length_2725_cov_3.062547"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="nrdD"
                     /locus_tag="PKN90_04160"
     CDS             complement(<1..915)
                     /gene="nrdD"
                     /locus_tag="PKN90_04160"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNZ85507.1"
                     /translation="METCIIKRDGKKENFSIEKIKSAIRKAFLSVGSFATDEVITHIL
                     SRVNITNGISVEDIQNQVEIALMAERYYAVAKAYMLYRQKHLEDREVKEKLEFLMEYC
                     DAKNAATGSKYDANANVENKNLATLIGELPKSNFIRLNRRILTDKLKEMYGKEVADRY
                     IDLLNNHYIYKNDETSLANYCASITMYPWLIGGTNSIGGNSKAPTNLKSFSGGFINMV
                     FMVSSMLSGACATPEFLMYMNYFIQLEFGDDYYLEADKVVDLSKKQRTLDKVITDYFE
                     QIVYSINQPTGARNFQAVFWNISYYDRYY"
     regulatory      complement(1052..1272)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1496..2068)
                     /locus_tag="PKN90_04165"
     CDS             complement(1496..2068)
                     /locus_tag="PKN90_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85508.1"
                     /translation="MCICFLFSCTTQISTSHHHPPSWSEPVIFGKKPNAIHYNYIPEA
                     SSQKTLAMVELIIIHIPQEYEGQLFTLAGGGVDVGLHGPNATGFSGVGIHFAGGNGNP
                     PRTIEYKKDYTKQVENGSLHFTIYYTPTDIIQHWDPTASRYVPMTEVTLTMHPLGEWN
                     SIFHSTINPYNWCIAGVGKGNKLYLEISLE"
     gene            complement(2095..>2725)
                     /locus_tag="PKN90_04170"
     CDS             complement(2095..>2725)
                     /locus_tag="PKN90_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNZ85509.1"
                     /translation="LGIITIDDVMDVVEEETTEDFHKFGAFQDAIDNPLKAKVSFLYK
                     KRIFWLTALIFMNIFSGAAIAHFQTTIQSVISLLFFLPLLIASGGNAGAQAATLTIRS
                     LAVGDVESKDWLHLVGKEFLVSLLLGITMALGVSLIASFRAPDIIAVVAITMTLTVMV
                     GSLIGLLLPFIFTRLKMDPASASAPLITSVADISGVLIYFTVARWILGL"
BASE COUNT          887 a          490 c          461 g          887 t
ORIGIN      
        1 ataatagcgg tcgtaatacg aaatattcca aaatacagct tgaaaatttc tggcaccagt
       61 aggttgattg atagagtaca caatttgctc aaaataatcg gtaattacct tatccaaagt
      121 acgttgcttt ttcgataagt caaccacctt gtctgcctca aggtaataat catcgccaaa
      181 ttctagttga ataaaataat tcatgtacat caaaaattcg ggagtggcac aagctccact
      241 cagcatgctc gataccataa acaccatatt gataaaacct ccgctaaagg attttaagtt
      301 ggtgggggct ttagagtttc ctccaattga gtttgtacca ccaatgagcc acggatacat
      361 ggtaatgctg gcacaataat tggctaagct cgtttcgtcg tttttgtata tgtagtggtt
      421 gtttagcaag tcaatatacc gatcggctac ttctttgcca tacatttctt ttagtttatc
      481 tgtaagtatg cgacggttga ggcgaataaa gtttgatttt ggtagttcgc caataagggt
      541 tgctaagttt ttattctcca cattggcatt tgcgtcatat ttgctgcctg tagctgcatt
      601 tttagcatcg caatattcca ttaaaaattc cagtttttct tttacttccc tgtcttccaa
      661 atgtttttgg cgatacagca tgtaggcttt ggctacagca taatagcgtt ctgccatcaa
      721 ggctatttct acttgatttt gaatgtcttc taccgaaata ccattggtaa tgtttacacg
      781 acttaaaatg tgtgtaatta cttcatctgt ggcaaaactg ccaacagata aaaaagcttt
      841 acgaatagca cttttgattt tttcaatcga gaaattttct tttttcccgt cacgtttaat
      901 gatacatgtt tccatactgt atataatttt gtaaataatt gctactaact tgcacgatga
      961 gacagtaggg atacaaacag acgaatggaa agtttctgaa taaatataga tttatcggag
     1021 aactatcctg ttgcctttca cccgaaagcc cagataataa tttacatacg gcaggtcttc
     1081 tgacttactc tcattttgaa aagccttccc attctacaaa tatgaacagt ggcataaggg
     1141 tttcaaaatt tctttacagg tcttctttaa gttgtaaaaa aagagtttac agctacgggg
     1201 atagttctcg aattgcacga gattcccatt ttaatccatc aataatatgg aaccgttagt
     1261 gcaacaaaaa tacagtattt ttttagaaag ttatccaaaa aaggaaagaa tagttattaa
     1321 cagaaataaa aaaaatctaa ttatttaata taatattttg gttaccaata gattgtattt
     1381 tatattattt atattaattt ctgaaaatgc aaataataga aaaataaagt agtagaaata
     1441 atgattttga ggataatcta acttgctaat tattttcaat gtaaattcta tttattcact
     1501 ctaaacttat ttcaagatac aatttatttc cttttcctac accagctata caccagttgt
     1561 atggattgat tgtagagtga aaaatactat tccattctcc tagggggtgc atggtaagtg
     1621 ttacttctgt cattggaaca tagcgagatg ctgttggatc ccaatgttga ataatgtctg
     1681 taggcgtgta ataaatggtg aaatgcaaac taccgttttc tacttgtttg gtgtaatctt
     1741 ttttgtattc aatggttcga ggtggattgc cattccctcc agcaaaatga attcccacac
     1801 ctgaaaatcc agtagcgttt ggtccatgta accctacatc tacaccacca cccgccaagg
     1861 taaatagctg tccctcatat tcttgaggta tgtgtatgat gattaattct accattgcca
     1921 aagtcttttg tgaagaagct tcgggtatgt agttatagtg tatagcattc ggcttttttc
     1981 cgaaaataac tggttcagac caactaggtg gatgatggtg tgatgttgat atttgtgttg
     2041 tgcaagaaaa taaaaaacat atacacatca atacgacaag ataatatgtt tgggttacaa
     2101 acctaatatc catctagcaa cagtaaaata aattaatact cccgatatat ctgctacaga
     2161 ggtaataaga ggagcactag ctgatgcagg gtccatttta agacgtgtaa aaataaatgg
     2221 caatagtaac cctattaaac tacctaccat taccgttaat gtcattgtaa tagcaacaac
     2281 agcaataata tcaggagctc taaatgatgc aataagactt acacctaaag ccattgtaat
     2341 acctaaaagt aaggatacta aaaattcttt cccaactaaa tgtaaccagt ccttactttc
     2401 tacatcaccc actgctaacg aacgtattgt tagggtggca gcttgagctc ctgcgttacc
     2461 accgctagca attagcaagg gtaaaaagaa taacaaagaa ataacacttt gaatagtcgt
     2521 ttgaaaatga gctatagctg cacccgaaaa aatattcata aaaattagag ctgtgagcca
     2581 aaatatacgt tttttataca aaaaagaaac ttttgccttt agagggttat caattgcatc
     2641 ttgaaaagct ccaaatttat gaaagtcctc tgttgtttct tcttctacca catccatcac
     2701 atcatcaata gtgataatac ctaac
//
LOCUS       DAOPUR010000191         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_38999_length_2715_cov_3.454511, whole genome shotgun sequence.
ACCESSION   DAOPUR010000191 DAOPUR010000000
VERSION     DAOPUR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38999_length_2715_cov_3.454511"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKN90_04175"
     CDS             <1..338
                     /locus_tag="PKN90_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNZ85510.1"
                     /translation="MALYNEILQPNGLIHLKTDSPFLYTYTQLMIEKNALDIHFQTDD
                     LYHSNWVNEILSIQTFYEKQWIERGLSIKYIQFKINKNTPLLEPEEEIEHDSYRSFNR
                     SKRSMQSTN"
     gene            351..1412
                     /locus_tag="PKN90_04180"
     CDS             351..1412
                     /locus_tag="PKN90_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNZ85511.1"
                     /translation="MAIYPQLIIDALRKVRYPGNNKDIVTNEMLEDDIRIEGNKVSFS
                     LILKANDPFAKSLVKAAEQAILTYVGEDIDIKGNISIKALPAPNPMLNPRSLTHIKHK
                     IAISSAKGGVGKSTVSSNLAVALANKGYKVGLLDADIYGPSIPKMFDIEEERPTVEVI
                     DGKDWIVPVEKMGIKILSIAFFIEKDNAIIWRGAMASNALKQLINDTLWGDLDFFLID
                     LPPGTGDVHLTLLQTIDLNGAIIVSTPQQVALLDTVKGINMFLNEKINIPIVGLIENM
                     SWFTPAELPNNKYYIFGKEGMKNLAENMKLPFLGQIPIVQSVCESGDSGSPIIINENS
                     IVGTSFDTITENILRNLKK"
     gene            1480..1836
                     /gene="rsfS"
                     /locus_tag="PKN90_04185"
     CDS             1480..1836
                     /gene="rsfS"
                     /locus_tag="PKN90_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNZ85512.1"
                     /translation="MTDTQQLVNSIIAGIQEKKGSKIVVADLSKLSNSICSYFIICEG
                     GSNTQVDAIASSLKDYVKKETKERPLGMEGLENCEWVVMDYGDVMVHIMQREPRKFYD
                     LENLWQDAKLTEIADI"
     gene            1874..2443
                     /locus_tag="PKN90_04190"
     CDS             1874..2443
                     /locus_tag="PKN90_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNZ85513.1"
                     /translation="MENENKQGKQPNNNKPKGKFIVNMYWFYGIIAAVLIALNIFMNS
                     DASKEVSYSEFRNYAEKGYVESIVVITNKNAAEVVIKQDSSILNTVFGDDKGKVNSLI
                     VTIPSPEKMEEFVADNDKNLNIKTDIKYEERKNYLDTLLWSFGPFILIIIIWIFIMRR
                     MSGEVLEATCLMLVNQKHNYSTKELTQCR"
     gene            2434..>2715
                     /locus_tag="PKN90_04195"
     CDS             2434..>2715
                     /locus_tag="PKN90_04195"
                     /inference="COORDINATES: protein motif:HMM:NF017844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ85514.1"
                     /translation="MSVTFKDIAGLAEAKQEIQEIVDFLKNPSKYTNLGGKIPKGALL
                     VGPPGTGKTLLAKAVAGEAHVPFFSMSGSDFVEMFVGVGASRVRDLFRQA"
BASE COUNT         1027 a          437 c          490 g          761 t
ORIGIN      
        1 ttatggcact gtataacgag attctacagc caaacgggct gattcaccta aaaacagaca
       61 gtccattcct gtatacctat acacaattaa tgattgagaa aaatgcatta gacattcatt
      121 ttcagactga cgatttgtat cattctaatt gggttaacga aatcctttcc atccaaactt
      181 tttacgaaaa acaatggata gagagaggat tgagtatcaa atatatccaa tttaaaataa
      241 ataaaaatac ccccttgcta gaacccgaag aagagataga acacgactct tacaggagtt
      301 ttaatagaag caaacggagt atgcaatcta ctaattaaac agataaaacc atggctatat
      361 atccacaact catcattgat gcacttagaa aagtgcgtta tcctggtaat aataaagata
      421 ttgttacgaa tgaaatgctt gaagatgata ttcgtattga aggaaataaa gtgtcatttt
      481 ctctgatact gaaagcaaat gatccgtttg caaaatcatt agtaaaagcg gcagaacaag
      541 ctatattaac ctatgtaggc gaagatattg atatcaaagg gaatattagt atcaaagcac
      601 tacctgcacc taacccaatg ttaaatccaa gaagtttaac ccatattaaa cacaagatag
      661 ccatctcttc agccaaagga ggtgttggaa aatcaactgt ttcatcaaac ttagcagtag
      721 cattggctaa taaaggatac aaagtaggat tgcttgatgc agatatctat ggaccatcca
      781 ttccaaaaat gtttgacatt gaagaagaaa gaccaactgt agaagttatt gatggtaaag
      841 attggattgt ccccgtagaa aaaatgggta ttaaaatcct ctctattgcc ttttttatag
      901 aaaaagataa tgccattata tggcgtggag caatggctag caatgccctg aaacaactta
      961 taaatgacac cctttggggc gatttagatt ttttcttaat cgatttacca ccaggaacag
     1021 gcgatgtaca ccttacgctt ttgcaaacca tcgatttaaa cggagccatt atcgtaagta
     1081 ctcctcaaca agtagccctt ttggatacag tgaaaggcat aaacatgttt ttaaatgaaa
     1141 aaataaatat tcccatagta ggtttaatag aaaatatgtc atggtttaca ccagcagaac
     1201 tgccaaataa caaatattat atttttggaa aagaagggat gaaaaatctt gctgaaaaca
     1261 tgaaattacc ttttcttgga caaataccta ttgttcagag cgtttgcgaa tcaggagata
     1321 gtggttctcc tattattatt aacgaaaata gtattgttgg cacaagtttt gatacaatta
     1381 cagaaaacat cttacgtaat ctcaaaaaat agaggtaatt aaaacagaaa tatttcatta
     1441 tccatacttt acaactatat ttgcagaaaa attaaccata tgacagatac acaacaatta
     1501 gtaaacagca taattgccgg aatacaagaa aaaaaaggct caaaaatcgt agtagctgac
     1561 ctctcaaaac taagtaacag catttgttcc tattttatca tttgcgaagg gggatcaaat
     1621 acccaagttg atgccattgc aagttcactt aaagactacg taaaaaaaga aacaaaagaa
     1681 cgaccattgg gcatggaagg tttagaaaat tgcgaatggg tggtaatgga ttatggtgac
     1741 gtaatggtac atattatgca acgcgaaccg cgtaaatttt atgatttaga gaatttatgg
     1801 caagatgcta aactaacaga aattgctgat atataaaaaa aatcaatatt gattaagatt
     1861 atttggaaaa aacatggaaa acgaaaataa acaaggaaaa caaccaaaca ataataaacc
     1921 caaagggaaa tttattgtaa atatgtattg gttttatggt ataattgcag ccgtactaat
     1981 tgctctaaat atatttatga attcggatgc aagtaaagaa gtatcttatt ctgaatttag
     2041 aaattatgcc gaaaagggat acgtagaatc gattgttgta atcaccaata agaatgctgc
     2101 tgaagtagta atcaaacaag actcatcaat tttaaacact gtttttggcg atgacaaagg
     2161 taaagtaaac tcattaattg taacaattcc ttctcccgaa aaaatggaag aatttgtagc
     2221 agataatgac aagaatttaa acataaaaac agacataaaa tacgaagaac gtaagaatta
     2281 tcttgacacc ttactttggt cgtttggtcc atttatactc atcattataa tttggatttt
     2341 catcatgaga aggatgagtg gtgaggtgct ggaggcaacg tgtttaatgt tggtaaatca
     2401 aaagcacaat tattcgacaa aggagctaac acaatgtcgg taacgtttaa agatattgct
     2461 ggattagccg aagctaaaca agaaatacaa gaaattgttg actttttaaa gaatccttct
     2521 aaatatacaa atttaggcgg aaaaatccct aaaggagcct tactcgtagg acctccagga
     2581 acagggaaaa cacttcttgc caaagcagtt gcaggagaag ctcacgtacc atttttctcg
     2641 atgtcgggtt cagactttgt agaaatgttt gtaggagtag gtgcctcgcg cgtaagagac
     2701 ttgtttcgcc aagcc
//
LOCUS       DAOPUR010000192         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_39025_length_2714_cov_2.495299, whole genome shotgun sequence.
ACCESSION   DAOPUR010000192 DAOPUR010000000
VERSION     DAOPUR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39025_length_2714_cov_2.495299"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2573)
                     /locus_tag="PKN90_04200"
     CDS             complement(<1..2573)
                     /locus_tag="PKN90_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNZ85515.1"
                     /translation="MKKIGIFITLLLSLTFVSFLLAKQQSVAMSYPTYWQQVDSLQTH
                     GMPKSANEIVQKIKQKALSEHAHEEIIRCLIYDLKTNVEIDHDSFFDQVAEIERYASS
                     SKDVISQSVLYAMLAQVYDSYFNANSYIIQQRTPIWDIVPENKKEWASNHFLYRIFHY
                     ASLSIQHEAALQKTLVTNYPTILVQATDSYLRPTVYDVLAHNYIQLYVQNKHQIERLV
                     TQEKSTEMSLITPYDHFIAEADTTTSLWQVVPRVKNVYQKLLTFHKASGNTDALVKTD
                     IDRIETLIALSIPSEKVSESYQLTLNSIIEKFQKSPIVVHAIVLKANYLLSENKRKEA
                     YDLCKLYINRFPDYSRIAVLHNIVEQIKQPQLATSSQSVVYPDSSLNLKLNFSNIDSL
                     TIRLYKIRSNSLEYQIVKNYYGKTRQAIDRELVNTIHVTLPVSELFLSKDTSIHIKTK
                     DLGLYEYEIVSPTNAFDSIKNTFYVTRLLLVHKTINKTIEGWVVDRITGKPINNATVT
                     VHQVRYQRNNVDVKKVASTNTNKNGLATFSLQHNNAYYLSVSQGEDTSTPIGNVHVYG
                     KQKENTPPDMQQHIALFTDRALYRPAQSVYVKGIAWNVKEDSVILEKNKKIHIRLRDA
                     NYQIIAEKQVQTSSYGSFNTDFILPKSGLTGSFTLETENGSSSFLVEEYKKPTFKAEI
                     KRIDKEVLFNNPVRINGNAETYMGFPLQTAHGKYRIVRQQFSWRRYFPIHTTENVAEG
                     VFKTNDKGEFTFDFIPIKPDTDQSAYYHYLVYVDITDKAGETQQVSSSFSIGESAIYI
                     QTNIPAIIQKEKITPIAVNVTNVNGEMLHQSGKIAIYALKESSNNDTPVDSLQLSKCV
                     Y"
BASE COUNT          826 a          445 c          455 g          988 t
ORIGIN      
        1 tcatatacac acttactcag ttgtagacta tcaacaggag tatcattatt tgagctttct
       61 tttaaagcat aaattgcaat tttgccactt tgatgaagca tttctccatt tacattggta
      121 acatttaccg ctattggagt tattttttct ttttgaataa ttgcaggaat attcgtttgt
      181 atatatatgg cactttctcc gattgaaaaa ctcgaagaaa cttgttgtgt ttcaccagct
      241 ttgtcggtaa tatcaacata tacaagataa tgataataag cactctggtc ggtatcaggt
      301 tttatcggaa taaaatcgaa tgtaaactcg cccttatcat ttgttttgaa aacaccttct
      361 gccacgttct ctgttgtgtg gataggaaaa tacctacgcc aagaaaattg ttgtcgaaca
      421 atgcggtatt taccatgtgc agtctgtaaa ggaaagccca tatatgtttc agcatttcca
      481 tttatgcgaa caggattatt aaacaatact tctttatcta ttcgcttaat ttcagcttta
      541 aaagtaggtt ttttatattc ttctactaaa aatgagctag agccattttc ggtttcgagt
      601 gtaaaactcc ctgttagccc tgattttggt aatataaaat cagtattaaa agagccgtaa
      661 cttgatgttt gcacttgttt ttctgcaata atttggtagt ttgcatcacg cagtctaatg
      721 tgtatttttt tgtttttttc taaaattaca gagtcttctt ttacattcca cgcaatccct
      781 tttacataca cactttgtgc tggacgatac aatgccctat ctgtaaacag tgcaatatgt
      841 tgttgcatat ctggcggggt attctctttt tgtttgccgt acacatgcac attaccgata
      901 ggcgtgctag tatcctctcc ttgcgatacg cttaggtaat aagcattgtt gtgctgtaac
      961 gaaaaggtag ctaatccgtt tttattcgta ttggttgatg ctactttttt tacgtcaaca
     1021 ttgttccgtt gatagcgaac ttgatgaaca gtaacagttg cgttgttgat aggtttacca
     1081 gttattctat ctactaccca cccctctata gtcttattga tagtcttatg caccagcaac
     1141 aaacgcgtta cataaaaagt attttttatg ctatcaaaag cattggttgg agatactatt
     1201 tcatattcat ataaacccaa gtctttggtt ttaatgtgaa tacttgtatc tttggataga
     1261 aacaattctg atactggtaa ggttacatgt atagtattca ctagttctct atcaatggct
     1321 tgacgggttt tgccataata attttttaca atttgatact ccaacgaatt actacgtatt
     1381 ttatataatc gaatggttaa tgagtcaata tttgaaaaat ttagtttcaa attcaacgat
     1441 gaatctggat acacaacaga ttggcttgat gttgctaatt gtggttgttt aatttgttct
     1501 actatattgt gtaatactgc aatgcgagag taatcgggaa aacggttgat gtataactta
     1561 cacaaatcgt atgcctcttt tcttttgttt tcactcaaca aatagtttgc ttttaaaaca
     1621 atagcatgta ccacaatagg agatttttga aacttttcaa tgatactatt taatgtaagt
     1681 tggtaagatt cagatacttt ttcagaagga atagataacg caattaaggt ttctattcta
     1741 tcaatgtctg tttttaccaa agcatcagta ttaccactcg ctttatgaaa tgtaagaagt
     1801 ttttggtata cattttttac tcgtggtact acttgccata gcgaagtagt tgtatctgcc
     1861 tcagcaataa aatgatcgta aggagttata agagacattt cagttgattt ctcttgtgtt
     1921 accaaacgtt caatctgatg tttattctgt acatataatt gtatgtaatt atgtgccaac
     1981 acatcatata ccgtaggtcg taaataacta tcagtagctt gcactaaaat ggtaggataa
     2041 ttggtaacaa gtgttttttg taaggctgct tcatgttgaa tagaaagtga agcataatgg
     2101 aatatcctgt ataaaaaatg attactagcc cactctttct tattttctgg tactatatcc
     2161 catataggcg ttcgctgctg aatgatatac gagtttgcgt tgaaatatga atcatatact
     2221 tgtgccaaca tggcatacag tactgattgt gaaataacat ccttagagga actggcatat
     2281 ctctctattt ctgcaacttg atcaaaaaaa ctatcatgat caatctctac attcgttttt
     2341 aaatcatata tcaaacaacg tataatttct tcgtgagcat gctcagatag tgctttttgt
     2401 ttgatttttt gtactatctc gttggcagat ttaggcatac catgtgtttg caaagagtcc
     2461 acttgttgcc aataagtagg atacgacata gcaacagatt gttgtttggc taacagaaat
     2521 gacacaaatg taagggaaag gagtaatgtt atgaatatcc ctattttttt catacgcaat
     2581 agtatatatt ttttgataga atgactacaa agtaacaaat tattaccgat actcagcata
     2641 aaataaaaca taaaaaaact tagctattga atattaacct aaaatttggg tatataaaaa
     2701 atttacgtta tctt
//
LOCUS       DAOPUR010000193         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_39431_length_2695_cov_2.787121, whole genome shotgun sequence.
ACCESSION   DAOPUR010000193 DAOPUR010000000
VERSION     DAOPUR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39431_length_2695_cov_2.787121"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKN90_04205"
     CDS             <1..412
                     /locus_tag="PKN90_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85516.1"
                     /translation="RYYEIETPHERLLDQLIYEGKIRYLLSIFFPEYTEYEIMGYSKE
                     QYEWSVKNEKNIWAYIIENKRLFSNNWREIAGFINPGPFTSGLSQESPGRIGIWIGFR
                     IVQQYAENNKDVTLEKLMEDNNSQRILELSGYRP"
     gene            complement(521..604)
                     /locus_tag="PKN90_04210"
     tRNA            complement(521..604)
                     /locus_tag="PKN90_04210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(568..570),aa:Leu,seq:gag)
     gene            complement(654..726)
                     /locus_tag="PKN90_04215"
     tRNA            complement(654..726)
                     /locus_tag="PKN90_04215"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Gly,seq:gcc)
     gene            complement(790..1919)
                     /gene="nspC"
                     /locus_tag="PKN90_04220"
                     /pseudo
     CDS             complement(790..1919)
                     /gene="nspC"
                     /locus_tag="PKN90_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
     gene            2169..>2695
                     /locus_tag="PKN90_04225"
     CDS             2169..>2695
                     /locus_tag="PKN90_04225"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HNZ85517.1"
                     /translation="MRKFTTTHQLRFGLIVLSLFSFLASHAITIYFKDTNNSWGGVNI
                     YIWDATIKTDNTSGWPGKAMIWKATNGSDTWYKYELQSTTASMKFNKVSDSDNTKTSD
                     FNNVSSDGWFDSGSSGPQDISGVPSGLNFELSLGAVTMSSDALDGWNVRTATTNAGGG
                     IFTLEKLYTQNLGID"
BASE COUNT          927 a          532 c          452 g          784 t
ORIGIN      
        1 acgttattat gaaatagaaa cgccacatga acgtttactt gatcagttaa tctatgaagg
       61 caaaatcagg tatcttctat ccatattctt tccagagtat acagaatacg aaataatggg
      121 atatagtaaa gagcaatatg aatggagtgt gaaaaatgaa aaaaatattt gggcatatat
      181 tattgaaaat aaacgattgt tttcaaataa ttggcgtgaa attgctggat ttattaatcc
      241 tggtcctttt acttctggac tctctcaaga atcgccagga cgcattggga tttggatagg
      301 tttccgtatt gtgcaacaat atgctgaaaa caataaggat gtaaccttag agaaacttat
      361 ggaagacaac aatagccaac gtattttaga gttatctggc taccgaccat aaaaaaaaag
      421 ctgtctgaat aaacttttaa accgctaaga atatattctt catcaaatac cctaataaaa
      481 agaggcagtc ttaaaatatt ctaagactgc ctcttttttg tgcacaagat gagactcgaa
      541 ctcacacagg ctttcgccca ctaccacctc aaagtagcgt gtataccaat ttcaccactt
      601 gtgcaatatt tcagatagaa aatgcgaaac ctccacgctt cgcattttta gagagcgaaa
      661 aacgggactc gaacccgcga ccctaacctt ggcaaggtta tgctctacca actgagctat
      721 tttcgcaaat atggtgcaaa gatacatgct aatcttgatt tttcaaaatt tgcaaaagaa
      781 aaaagtatat tatgccatcc tctgcttata gtcaaggtaa gtaaaaacac gaaacaactc
      841 aaacttatct tctagtgtcc acataccaat acttggatgc gacacaccat taaacatggt
      901 tgttttgacc attgtataat gaatcatatc ttcaaacaca attttatctc caacttttag
      961 tggtttttca aacgaccact cgcctacgaa atcgcccaca gacaactatt accccccatt
     1021 cgatacgttg gtttaccttc aataacatct gtagctccaa caatacgcgg tttataaggc
     1081 atttctaagc aatcgggcat atgacaagca aacgacacat ctagcatagc cgtagaaaca
     1141 cccctattct ctacaatatc caacacggta gaaaccaaca cccccgtttg ccacaagaaa
     1201 gcactccctg gctctaaaac taactgaata tgaggatact tgcttttgaa tgattttaat
     1261 agattgatta aatgctcggt atcgtaacct ttgcgagtca ttaaatgtcc tccacccata
     1321 ttcaaccatt taatttgaga aaagaaacga ccaaactttt gttctacaac ttctaaagta
     1381 gcttctaagt caaacgaact tgattcgcat aaagtgtgaa aatgcaagcc ttcaattccc
     1441 tgaggcaaat tatcatccaa attagcaacg gaaacaccta agcgggaacc aactgcacag
     1501 ggattgtata aatctgtttc gacagaagaa tactcaggat tgatgcgtaa gccacacgaa
     1561 attgacttgc tcttacaaac agcagcatac tgattgtact gtgctactga attaaacgtg
     1621 atatgactac tacacgcagc aatagcatca aactcttctg gcttatatac gggtgcatac
     1681 gtatgagctt tgctccccat ctcttcgtaa gccaaacgtg cctcactcaa agaacttgcc
     1741 gttgaatatg gaatatattc tcgaataata ggaaacactt tccacattgc aaaagcttta
     1801 aatgcaagaa taatttcaac ttccgcttgc tctttaacag actgaataag gagtaaatta
     1861 gcacgcaaca acttttcgtc aagcacaaaa caaggagaag gaatctgtga taggttcata
     1921 cggatatttt ttttttacaa aagtacagaa atttagttta tttacgaagt gaaaccgaaa
     1981 aataccatgt aaaaccgaag tatatgctac aaccgataag tcttaactaa gttttcttgt
     2041 atattttgat ttttttatgt ttatttgcaa gttattaaat aatgtgccgt tttttgcata
     2101 gaaaacacaa aactattaca aaaataaaaa aatatctaaa ccataaccac ctactatacc
     2161 agaatactat gagaaagttt acaaccacac atcaactacg ttttggatta atcgtattga
     2221 gtttgttttc ttttttggct tcacacgcca tcactattta ttttaaagac accaataatt
     2281 cttggggagg agtaaatatt tatatctggg atgctaccat taagacagac aatacatcag
     2341 gatggcctgg aaaagctatg atttggaaag ccactaatgg ttcagatacc tggtacaaat
     2401 atgaacttca gagcactact gccagcatga aatttaacaa ggtttcggat tctgacaaca
     2461 ctaaaacttc ggattttaac aatgtatcgt ctgacgggtg gtttgattca ggaagtagtg
     2521 gacctcagga tatttcaggc gttccttctg gattaaattt tgaattatcc ttaggtgctg
     2581 taacgatgtc ttctgacgca ttagatggtt ggaatgtaag aacagccaca accaatgccg
     2641 gaggaggaat ttttacatta gagaaattat atacacaaaa tttagggatt gatat
//
LOCUS       DAOPUR010000194         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_39446_length_2694_cov_2.646457, whole genome shotgun sequence.
ACCESSION   DAOPUR010000194 DAOPUR010000000
VERSION     DAOPUR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39446_length_2694_cov_2.646457"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2694)
                     /gene="porU"
                     /locus_tag="PKN90_04230"
     CDS             complement(<1..>2694)
                     /gene="porU"
                     /locus_tag="PKN90_04230"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNZ85518.1"
                     /translation="KEGTLSTYTVALDNAVFTSSTEDETTVLSQLGVPVQTTIHIDYC
                     IVYEKKQPSLQLLFTPIIQKENSYAKLIKADLIISSTPANKTGNQGKRSLGDEDQSIH
                     QYASSSVLASGNWYQIRVPQTGIYKLTYEDLLSLGIQNPAQVRVFGYGGAILEEDFSI
                     PRVADDLPEIALFMEKGSDDVFNAGDYILFHAQGTIRWQYDKYSGYYKHQQNYYATHG
                     YYFVTERAGEGKRITSQPLVTETPVATVTDFLQYGVYEKEQVALLQSGRKWYGDKFTN
                     TNRVLTIPFSFPSVSTTKSAKIDISVVGNSTQSSYISVHIDDEAQINKMSIFAITATN
                     EVAREVNSTYYHMPKATSEINVKLTYAPENATAFAHLDFVRVNAYQALKMQGNSMYFR
                     QTDQNVRYDVLQYELGNATSDIRIWDLSDPLNMVEIPTTFSTNTLFFVPSVRSDLLVR
                     EFLAIRTSSTFPKPEVIGKIANQNLHAIQQVDYVIISPNEFREQAMRLATFHQQKNGY
                     SVVIVEPQQIYNEFSSGTPDATAYRWFLKMLYDRAGDNINNQPKALLLFGLSSHDNRG
                     LVYSRMPLLSYQSFNSLDSHISYTTDDYYAFLDDSEGVNIIQDIMDVGVGRLPVATKE
                     EAKIVVDKIINYANNTKKGSWKNLLAFLGDDGDSNDHMEQSDTLVQKCQEKWNEYQPI
                     KVFLDSYQMTQTASGSTYPGARDRILNLLKSGVLIFNYVGHGSVNSLTEEQTIVRKDI
                     ENMRNENLALWVTATCDFSRYDNNKTSAGMNVLLNPYGGGIGLLTTTRTVYSFQNYNL
                     SKQIYRYLLPEDPFNPMPLGEIMRRAKVGMGADENKLNFALLGDPMLSLSYPTNRVIT
                     TQINDAVLPDIATIKSLSLVTIKGYIENNEQNGILEEFNGF"
BASE COUNT          843 a          521 c          444 g          886 t
ORIGIN      
        1 taaaaccatt aaactcttct aaaattccat tttgctcatt attttcaatg tatcctttta
       61 ttgtaactaa gctcagtgat tttatagtag ctatatctgg caaaacagca tcatttattt
      121 gggtagttat aaccctattt gttggataag ataaagatag cataggatcg cccagtagtg
      181 caaaattcaa tttattttca tccgcgccca taccaacctt tgctctacgc ataatttcac
      241 ctaatggcat tggattaaaa ggatcctctg gcaaaagata tcgataaatt tgcttactta
      301 aattataatt ttgaaatgaa tataccgtac gcgttgtagt taataagcct atacctcccc
      361 catatggatt aagcaataca ttcattcctg cagatgtttt attattgtca tatcgactaa
      421 aatcacatgt tgccgttacc cacaaggcta gattctcatt acgcatattt tcaatatctt
      481 tacgcacgat ggtttgttcc tcagtaagcg agtttacact tccgtgtccc acgtaattaa
      541 aaatcaacac tccagacttg agcaaattga gtatcctgtc tctggctccg ggatatgtac
      601 ttccacttgc agtttgcgtc atttgatatg agtctaaaaa cactttaatt ggttgatatt
      661 cattccattt ctcttggcat ttttgcacta atgtgtcaga ctgttccatg tggtcattgg
      721 aatcaccatc atcccctaaa aaagcaagca aatttttcca agatcctttt tttgtattgt
      781 tggcataatt gataatttta tctaccacta ttttagcttc ttctttagtt gcgacaggca
      841 gacgacccac cccaacgtcc ataatatctt gtatgatatt taccccctct gaatcatcca
      901 aaaaagcata ataatcgtca gttgtataag aaatatgaga gtctaaagaa ttgaatgatt
      961 gataactcaa taaaggcata cgagaataca caagcccacg attatcgtga gatgataatc
     1021 cgaaaagcaa caaagcctta ggttgattgt tgatattatc tccagctcta tcatacaaca
     1081 tttttagaaa ccatcgatac gctgtagcat caggtgttcc tgaagaaaat tcattataaa
     1141 tctgctgagg ttctactatc actacagaat aaccattttt ttgttgatga aatgtagcca
     1201 aacgcattgc ctgttctcta aattcatttg gagaaataat tacataatcc acttgttgaa
     1261 tcgcatgtaa attttgattg gctattttac caattacttc tggttttgga aatgtagacg
     1321 acgtgcgtat agccaaaaat tctcgcacca acagatcaga gcgaacagaa gggacaaaaa
     1381 acagcgtatt agtcgagaat gtagttggta tttctaccat attcagagga tcggataaat
     1441 cccagatacg tatatccgaa gtagcatttc ctaactcata ctgcaataca tcataccgta
     1501 cattttgatc tgtttgccta aaatacatac tattcccttg cattttaaga gcttgataag
     1561 catttacacg aacaaaatca agatgagcaa aagcggttgc attttcagga gcataagtaa
     1621 gttttacatt aatttcggaa gtcgcttttg gcatatgata ataggtacta tttacttctc
     1681 gtgcaacttc attggtagcc gtaatagcaa aaatagacat tttattgatt tgtgcctcat
     1741 catctatatg cacacttatg taactactct gagtagaatt tcccaccact gaaatatcaa
     1801 ttttagctga tttggttgta cttacagaag ggaatgaaaa ggggattgtt agtaccctat
     1861 ttgtattggt aaatttatct ccataccatt tcctccctga ttgaagtaaa gctacttgtt
     1921 ctttctcata cactccatac tgtaagaaat ccgttaccgt agcaacagga gtttctgtaa
     1981 caagaggctg agatgttatg cgtttgcctt ctcctgctct ttctgttaca aaataatatc
     2041 catgggttgc ataataattc tgttgatgtt tataataccc actatactta tcgtattgcc
     2101 agcggatagt accttgtgca tgaaacaaaa tataatctcc cgcattaaac acgtcatcag
     2161 atcctttttc cataaatagt gcaatttctg gcaaatcgtc agctacacga ggaattgaaa
     2221 aatcttcttc caagatagct ccaccataac caaaaacacg cacttgagcc ggattttgta
     2281 tacctagtga caacaaatcc tcataggtta atttataaat accagtctgt ggcacacgaa
     2341 tctgatacca attaccagaa gccaatacag atgatgatgc atattgatgg atagattgat
     2401 cttcatcacc taatgatcgt tttccttgat tcccagtttt atttgcagga gtagaagaga
     2461 taatcaaatc tgctttaata agttttgcgt aagaattttc cttttgaata attggagtaa
     2521 aaagaagttg aagggatggt tgtttttttt cataaacaat gcaataatca atgtgtattg
     2581 ttgtctgtac gggtactccc aattgggata acacagttgt ttcatcctcc gtagaagaag
     2641 taaaaacagc attatcaagg gcaacggtat aggtagaaag tgttccttct ttgc
//
LOCUS       DAOPUR010000195         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_39544_length_2689_cov_3.237661, whole genome shotgun sequence.
ACCESSION   DAOPUR010000195 DAOPUR010000000
VERSION     DAOPUR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39544_length_2689_cov_3.237661"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PKN90_04235"
     CDS             <1..539
                     /locus_tag="PKN90_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HNZ85519.1"
                     /translation="KLKPIHGVVYFVILLISCHFAWKMAFVEHTEIPRKVTFLWFDVT
                     QIFSFAVHNLTDVVYFLLKDILDVNIIKISNFFVYPDKAVIDIVWSCSGFKQAYIFFT
                     VIAFYPGKHIHKLWYIPLGLVVVYVFNVFRIALITYLLRDNLGWFDVLHEGSKYVFYA
                     IIFLIWVIWAEKFNKSSI"
     gene            545..1273
                     /locus_tag="PKN90_04240"
     CDS             545..1273
                     /locus_tag="PKN90_04240"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNZ85520.1"
                     /translation="MNLVIDCGNSTTKIAVFDGENIISKSVVVTLHITDITSILHLYP
                     IKNAVISNVGIIPTDCIELIRKSVVVYIDLQSTTPIPLKNNYETPSTLGLDRLAVAVG
                     ANYLYPSRNILVIDAGTAITYEFIDEYNTYQGGSISPGLVTRFKSLHHFTQRLPLVQP
                     ENTIELFGRNTRAAILTGVINGLVYEIEGYITDFKQNYRDVLIFLTGGDIFYFDKKLK
                     SSIFVSENLLLIGLNRILNFNACL"
     gene            1264..2508
                     /locus_tag="PKN90_04245"
     CDS             1264..2508
                     /locus_tag="PKN90_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85521.1"
                     /translation="MFINKHIAIILSLTLISIHLVAQSGTVSPYSRYGYGNLRSGGFT
                     VSNSLGGVGYGLRSNSMINPQNPASYTAVDSLSFMFDFGASASFNQFTSGFSQQQKST
                     AQFDYLALQIPIIKNMGMSLGLMPFSTVGYAYSFSETLQFNADELLTKHTFEGNGGIS
                     RLYGGLAYAIMPQLSLGVNVNYLFGNIVHTRELTFPDNSTYSSSKEESNLYVSTFLWD
                     AGIQFIQPVGKKDVLVVGGTYTLKTPMNIRSEIVTTSTQTITDDTNYSFDYPQSIGVG
                     VSYKHGTSVVFAADHSYQSFADALFYSQRDTLLNARRLAIGCEYVPNSTSRSYVQTIR
                     YRLGANIAESYTKVNEKPYSEMGITLGFGLPLKNSKSILNLNLEYGSRGIINQNLVRE
                     DYVKIGLGVSINENWFFKRKFE"
BASE COUNT          853 a          386 c          512 g          938 t
ORIGIN      
        1 aaaaattgaa acccatacac ggtgtagttt attttgtcat cttattgatt tcatgtcatt
       61 ttgcatggaa aatggcattt gtggaacata cagaaatacc acgtaaagtt actttcttgt
      121 ggtttgatgt tacccaaatt ttttcttttg cagtacataa tctgacagat gttgtgtatt
      181 ttcttttgaa agatattttg gatgtgaata ttataaaaat tagtaacttt tttgtgtatc
      241 cagacaaagc tgttattgat attgtttgga gttgtagtgg ctttaagcaa gcgtacattt
      301 tttttacagt catagctttt tatccaggaa aacatattca taaactgtgg tacattccac
      361 ttggtctagt tgtagtatac gtttttaatg tatttcgtat tgccctcatt acgtatttat
      421 tacgtgataa tttgggttgg tttgatgttt tgcacgaagg ttctaaatat gtattttacg
      481 cgattatctt tttaatatgg gtaatctggg cggaaaaatt taacaaatca tccatatagt
      541 ctcaatgaat ctagtaattg attgtggtaa ttctactact aaaatagcgg tttttgatgg
      601 agaaaatatt atttcaaaat ctgttgttgt aacattacac ataacggata ttacaagtat
      661 attgcatcta tatccgatta aaaatgcggt tatttctaat gtaggtataa ttcctactga
      721 ttgcattgag ctgataagga aatctgttgt tgtttatata gaccttcagt caacaacccc
      781 tattccacta aaaaataatt atgaaacacc atctacgtta ggtttagata ggttagcagt
      841 tgcagtaggt gcaaattatt tatatccatc acgcaatata ttagtgatag atgctggtac
      901 cgccatcaca tatgagttta tagatgaata taatacatat caaggcggaa gtatttcgcc
      961 tgggttggtt actcgtttta aatcgctaca tcattttaca caacgattac ctttggttca
     1021 gcctgaaaat acaatagaac tatttggaag aaatactcgt gctgctattt taactggggt
     1081 aataaatgga ttagtatatg aaatagaagg gtatattact gattttaagc aaaattatag
     1141 agatgttttg atttttttaa caggaggaga cattttttac tttgataaaa aattaaaaag
     1201 tagcatcttt gtatccgaga atctactcct aataggtttg aatcgtattt taaattttaa
     1261 cgcatgttta taaacaaaca cattgcaatt atactgtcgc taacattgat aagtatacat
     1321 ttagtagcac aaagtggtac ggtttcgccc tattcaagat atggttatgg caacctacgt
     1381 tcaggaggtt ttactgtgtc taattcactc gggggagttg ggtatggact tcgttccaac
     1441 tctatgatta atccacaaaa tcctgcatca tataccgctg ttgatagtct ttcttttatg
     1501 tttgatttcg gtgcatctgc ttcttttaat cagtttacta gtggctttag tcaacaacaa
     1561 aaatcaacag ctcaatttga ttatttagca ttgcagattc ccattatcaa aaacatgggt
     1621 atgagccttg gattgatgcc gttttctacg gttggttatg catatagttt ttcggaaacc
     1681 ttacagttta atgctgacga attattgaca aagcacacgt ttgaaggtaa tggaggaatt
     1741 tcaaggttgt atggaggatt agcgtatgcc ataatgccac aattatcatt aggcgtaaat
     1801 gtaaattatc tttttgggaa tatcgtacat actagagaac tgacattccc agataattct
     1861 acttactcct catcaaaaga agaatctaat ttgtatgtaa gtaccttttt gtgggatgcg
     1921 ggaatacaat ttatccaacc ggttggcaaa aaagatgtct tagttgttgg tggaacgtat
     1981 acactcaaaa cgccaatgaa tattcgtagt gaaattgtta ccacatctac ccagaccatt
     2041 accgatgata ccaactattc atttgattat ccacaatcaa taggggtagg tgtaagctac
     2101 aaacatggga ctagtgtagt atttgctgct gatcattctt atcaatcatt tgctgacgca
     2161 cttttttata gccagagaga tactttgctg aatgcgagga gattagccat tggttgtgag
     2221 tatgttccaa atagtacatc tcgctcttat gtgcaaacta tacgttatcg tttgggagca
     2281 aacatagcag agtcctatac aaaagtaaat gaaaaaccat attcagagat ggggattacg
     2341 ttaggatttg ggcttcctct taaaaattct aaatcaattt taaatttgaa tttggaatat
     2401 ggtagcagag gtataattaa tcaaaatttg gtgcgtgagg attatgtaaa gattggactg
     2461 ggagtgtcta taaatgaaaa ttggtttttt aagaggaaat ttgaatagta gctacaataa
     2521 aaacagtagg tcagattgaa aaaaaattaa aaataaaggt atgaaagcaa agttaatttt
     2581 tattggttta gtcgtgttga gtgtattcac ggtaaatgct caaaaaggtg ttgatacggg
     2641 aactcgatat gggttaggag aagatagtat caggtgttta gaaaacatc
//
LOCUS       DAOPUR010000196         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_39673_length_2682_cov_3.047963, whole genome shotgun sequence.
ACCESSION   DAOPUR010000196 DAOPUR010000000
VERSION     DAOPUR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39673_length_2682_cov_3.047963"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKN90_04250"
     CDS             complement(<1..841)
                     /locus_tag="PKN90_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85522.1"
                     /translation="MKTAKRILICPLNWGLGHATRCIPIIHKLQQEGNILLIASSGCA
                     GELLQQEFPTLEYIHFPGFSITYSKHHSQTLRIVLSFPKIIYYSFKEHFVLKKLIKIH
                     DINCVISDNRFGLWNKDITTAYITHQLNIKLPKHVKIFEKLTWYIHRYFIEKYNYCII
                     PDNQQSTLSLAGDLVHQRKLPKNAFFCSPLSRFSKSIQNTSTKFQSNILIILSGIEPQ
                     RSILEKLLLQQLTKINQKAILIQGLPNETKYKKNIGNITIISFVLHQELQELINNTDL
                     VICR"
     gene            complement(838..1605)
                     /locus_tag="PKN90_04255"
     CDS             complement(838..1605)
                     /locus_tag="PKN90_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ85523.1"
                     /translation="MLFSSIIFGPIKSRRLGNSLGINLLPTDAKICSFDCIYCECGLN
                     DSTKKGNIPTREAVKNALSLELEKLKKQDITIDTITFAGNGEPTMHPDFKEIIDDTLI
                     IRNQLAPDAVISVLSNAWHITNPKVYGALLKVDNNILKLDSAIKETILQINHPNNTSF
                     EVSELIANLASFQGQCIIQTLFVKGNHKGNLIDNTTEEEINAWLNALKTIQPKQIMIY
                     SIDRETPIKSLEKIPSSTLKAIASQAKKLGFNISVSV"
     gene            complement(1617..>2682)
                     /locus_tag="PKN90_04260"
     CDS             complement(1617..>2682)
                     /locus_tag="PKN90_04260"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNZ85524.1"
                     /translation="VLTDKLGDMGKKIHSGRSRNDQVLLDLKLFSRAKIQEIATETHL
                     LFNTLLTQSEQYKSVLMPGYTHLQIAMPSSFGLWFGAYAESLVDDMQLLLAAFKVCNR
                     NPLGSAAGYGSSFPLNRQKTTDLLGFDSMNYNVVYAQMGRGKMERTVSTALASIANTI
                     AKLAFDSCLFTSQNFGFIKLADEFTTGSSIMPHKKNPDVFELTRAKCNKIQGIPQQIN
                     LITNNLPSGYFRDLQIIKELFIPMFDELIECIRMTRYIIEKVEINTHILDDKKYQAIF
                     SVEEVNKQVLSGIPFRDAYKNIGLAIEKGDFHPDTSISHTHEGSIGNLCNEEINSLMN
                     EIISAFNFKSIQEAEAKLLL"
BASE COUNT          795 a          412 c          481 g          994 t
ORIGIN      
        1 agcgacaaat taccagatct gtattgttaa tcaattcttg taattcttgg tgtaaaacaa
       61 aggatataat cgttatattc cctatgtttt ttttgtattt agtttcgttt ggcaatcctt
      121 gaatcaagat ggctttttgg tttattttgg taagttgttg caataagagc ttttctaaaa
      181 ttgaacgttg aggttcaatt ccagataaaa tgataagaat gtttgattga aattttgtag
      241 atgtattctg gatacttttt gaaaagcgtg atagaggaga gcaaaagaaa gcattttttg
      301 gcagttttcg ttggtgaact aaatctcctg ctaatgataa agtactctgt tgattatctg
      361 gaatgataca ataattatat ttttcgataa aatatcgatg tatgtaccaa gttaattttt
      421 caaagatttt gacatgttta ggtagtttaa tgtttaactg atgggtaatg tatgctgttg
      481 taatatcttt attccatagc ccaaaacggt tatctgatat tacgcaatta atatcatgta
      541 ttttaatgag tttttttaat acaaagtgtt ctttgaaaga gtaataaata atttttggga
      601 atgacaatac aatacgtaga gtctgtgaat ggtgtttgct atatgtaata ctaaatccag
      661 ggaaatgtat gtattcaagc gttggaaatt cttgttgaag taattctcct gcacatccac
      721 tactagcaat tagtagtata tttccttctt gttgcaattt atgtattatc ggaatgcagc
      781 gagtggcgtg acctaatccc caattaagag gacagattag tattcgctta gctgttttca
      841 tactgaaaca gaaatattaa atcccagttt tttggcttgt gaggcaattg ctttcaatgt
      901 cgaactaggt attttttcta agcttttaat aggtgtttct ctatcaatag agtaaatcat
      961 aatttgtttg ggctgaattg ttttgagggc atttagccat gcattaattt cttcttcggt
     1021 tgtgttgtca atcaaatttc ccttatggtt tccttttaca aagagagttt ggattataca
     1081 ttgaccttga aaagaggcta aatttgcaat tagttccgat acctcaaagg atgtattatt
     1141 ggggtggttt atttgtaaaa ttgtctcttt tatagcacta tccaatttta agatgttatt
     1201 gtctactttt agcagagccc catatacttt tgggttggta atgtgccatg catttgaaag
     1261 aacagagata acagcatctg gagctaattg attacgtatt atcaacgtgt catctataat
     1321 ttctttaaaa tctggatgca ttgttggttc accattgcca gcaaaagtaa tagtatctat
     1381 tgttatatct tgttttttta gtttttctag ttctaaagaa agagcatttt ttacagcttc
     1441 tcgtgtagga atattgcctt tttttgtact atcatttagt ccgcattcac aataaatgca
     1501 atcgaatgaa caaatttttg catcagtagg taacaggttg attccaagtg aattacctag
     1561 tcttctgctt tttataggac caaatataat tgaactgaat aacataatga gtggttttac
     1621 aatagtaatt tcgcttctgc ctcttgaata cttttgaaat taaatgctga tataatctcg
     1681 ttcattaaag aattaatttc ttcgttgcat aagtttccaa tgctcccttc atgcgtatga
     1741 gaaatgcttg tgtctgggtg aaaatctccc ttttcgatgg cgagaccaat gtttttgtaa
     1801 gcatctctaa aaggaatacc agataatact tgtttattta cttcctcaac actaaagatg
     1861 gcttgatatt tcttgtcatc taaaatgtgt gtgtttattt ctactttttc gatgatataa
     1921 cgagtcatcc gtatacattc tattagctca tcaaacatcg ggatgaataa ttctttgatg
     1981 atttgcaaat cgcggaaata tcctgaaggt aaattgttgg taattagatt aatttgttgt
     2041 ggaatacctt gtattttatt gcatttggca cgcgttaact caaatacatc tgggtttttt
     2101 ttgtgaggca taatgctcga ccccgttgta aattcgtcag ccaatttaat aaatccaaaa
     2161 ttttgactgg taaacaaaca actatcgaaa gccaatttgg ctatcgtgtt agcaatggat
     2221 gctaatgctg ttgatactgt acgctccatt tttcctctac ccatttgagc atacactaca
     2281 ttgtaattca tggagtcaaa tcccaataaa tcagtagttt tttgtctgtt aagagggaaa
     2341 gaagaaccat atccagcggc agaacccaat gggttgcgat tacatacctt aaatgctgct
     2401 agtaatagtt gcatatcatc aactaaactt tctgcgtaag ccccaaacca tagtccaaat
     2461 gacgaaggca tagctatttg taggtgtgta tacccaggca taagaacgct tttatattgt
     2521 tcactttgtg ttaataaagt attgaacagt aagtgagttt ctgttgcaat ttcctgtatt
     2581 ttagctcgag aaaaaagctt caaatcaagt agaacttggt cgttgcgtga gcgtccacta
     2641 tgaatttttt tacccatatc acccaactta tctgtaagca ct
//
LOCUS       DAOPUR010000197         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_39676_length_2682_cov_2.710697, whole genome shotgun sequence.
ACCESSION   DAOPUR010000197 DAOPUR010000000
VERSION     DAOPUR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39676_length_2682_cov_2.710697"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PKN90_04265"
     CDS             complement(<1..1404)
                     /locus_tag="PKN90_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNZ85525.1"
                     /translation="MSIKVKQRDITDCGAACLASVASHYKLELSVARIRQMAGTDTKG
                     TNVLGIVQAAEKLGFIAKGVKGDKDVLTKVPLPSIAHIVLEKDGMQFYHFVVIYKVSK
                     NKISYMDPSSGTIITKIIDDFAPSWTGVLVILLPNDDFIARNEKISNFQRFIFLLAPH
                     KSILLQSLIGAILYTILGLSTSIFIQKITDNVLPSGNTNMLNLLSVGMLLVLILQVLL
                     GIYQTLFMIKTGQKIDMRLVLGYYKHLLKLPQSFFDTMRVGEIISRINDAVKIRAFIN
                     DVSISLTVNLFIVLFSFVLMFIYSWKLALLMLLVIPLYTVVYIITNKLNKKQERKLME
                     HSADLESQLVESINSVRTIKQFGMEDFSNMKTETRFITLLKTVFKSGKNSLFSTYSSE
                     IISRIFTIVLLWIGTYFVIDNEITLGELLSFYAIIGYFTGPVASLITANKSIQNALIA
                     ADRLFEIMDLEREITENK"
     gene            complement(1465..2610)
                     /locus_tag="PKN90_04270"
     CDS             complement(1465..2610)
                     /locus_tag="PKN90_04270"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ85526.1"
                     /translation="MQQLFPSEIIENTVESYHTQINTRSRAIYGIIVAIVLLIIASLP
                     FVYIDVTTPSRGVIRTPSENTTIQSAVYGQITMYNLYENKTVLMGDTLLLLNSENLTE
                     QQTLIESKLTENRNFISDIDKLLTKNYKALVTPKYISEYKRFTTKTNEREVTINFLKK
                     DLETTHRLMEKNVIASNEYLLAKNNYEKALEELNSIRQEYWTTWQAEQTNLKIQNLEL
                     ESKRKQIEDEKKQYTIVSPISGILVQVAGYRKGNFIAPGQTLCYISANDSLIAECYIS
                     PADIGYIFPNQPVSIQLDAYNYRQWGMIDGCVTEVLQDVVLLDNKPMFRVRCTIDQKY
                     VQLKNGYKGNIQKGLTFTAQFKLNRRSLWQLLFDKLDNWLNPKMMKE"
BASE COUNT          803 a          460 c          449 g          970 t
ORIGIN      
        1 cttattttcg gtaatttcgc gttctaagtc cataatttca aataaacggt cggcagcaat
       61 cagtgcattt tgtattgatt tgtttgctgt tatcaggctt gctacaggac ccgtgaaata
      121 tccgataatg gcatagaatg atagtaattc tcccaacgtt atttcattat caattacaaa
      181 atatgtgcct atccatagta acacaatggt aaatatccta ctaattattt ctgatgaata
      241 agtagaaaaa agtgagtttt tcccagattt aaacaccgtt tttagcaagg ttataaatcg
      301 cgtttcggtt ttcatgttac taaaatcctc cataccaaat tgtttgatgg tacgtacaga
      361 atttattgat tcgactaatt ggctttccaa atctgccgaa tgttccataa gtttacgctc
      421 ttgtttttta tttaacttat tagtaataat ataaactaca gtataaagtg gtatcactaa
      481 caacattagt aatgctaact tccaactata tataaacatt aaaacaaacg aaaacaaaac
      541 gataaacaaa ttaactgtaa gcgaaataga aacatcgttg ataaaagcac ggattttaac
      601 agcgtcgttg atacgtgaaa ttatttcgcc tacacgcatg gtatcgaaaa aactttgtgg
      661 gagtttaagt aaatgtttgt aataacccaa tacaagacgc atatcaattt tctgccccgt
      721 ttttatcata aaaagggtct gataaatacc caataagacc tgtaatatca acaccaatag
      781 catacctacg cttaataagt tcagcatatt tgtattgcct gatggtaaaa cattgtcagt
      841 tattttttgt ataaaaattg aagtgcttaa cccaagtata gtataaagga ttgcgccaat
      901 aagactttgt aatagaatgc ttttatgtgg agcaagaaga aagataaaac gttgaaaatt
      961 tgatattttt tcattacgtg caataaaatc atcgtttggg agtagtataa ccaatacccc
     1021 tgtccatgaa ggagcaaaat catcaataat ttttgtaata attgtgccac tagatgggtc
     1081 catataactt attttatttt ttgacacttt gtatattaca acaaaatgat aaaattgcat
     1141 cccgtctttt tctaaaacaa tatgtgctat tgatggtaac ggaactttgg ttaaaacgtc
     1201 cttatctcct tttactcctt ttgcaataaa accaagtttt tcagcggctt gtacaattcc
     1261 cagtacattc gtccccttag tatctgtacc tgccatttgt cgaatgcgtg ccacagataa
     1321 ttcgagtttg tagtgcgagg ctactgatgc tagacaagct gcaccgcaat cagtaatgtc
     1381 tcgctgtttt actttgatac tcataagaat ttattttgtt gtttattttc caaccttgcg
     1441 agttggtgct tatgttggac ttatttactc cttcatcatc tttgggttta accaattgtc
     1501 aagtttgtcg aatagcaatt gccaaagcga tcggcggttg agtttgaatt gtgctgtaaa
     1561 agtaagtccc ttttgaatat tccctttata gccattttta agttgtacat atttttggtc
     1621 tattgtgcaa cgaaccctga acattggttt attatccaat aaaacaacat cttgcaatac
     1681 ttcagtcaca catccatcta tcatacccca ttgtctataa ttatatgcat ctaattgaat
     1741 tgaaacgggc tgattgggaa atatgtagcc aatatcagca ggagaaatgt aacattctgc
     1801 gattaaagaa tcatttgccg aaatgtagca tagtgtttgt ccaggtgcta taaaattacc
     1861 ttttctatac cctgctactt gcaccaatat gccggatatg ggcgacacta tggtatattg
     1921 ttttttttca tcttcaattt gctttctttt cgactctaat tccagattct gaatttttaa
     1981 attagtttgt tcggcttgcc atgttgtcca atattcttgt cgaattgaat ttaattcctc
     2041 caacgctttt tcataattgt ttttggctag cagatactca ttgctagcta ttacattctt
     2101 ttccataagc cgatgggttg tttccaaatc tttcttcaaa aaatttattg ttacctcccg
     2161 ttcgttcgtt tttgttgtaa atcgtttata ttcacttatg tactttggag taacaagggc
     2221 tttgtaattc ttcgtaagca atttatcaat gtcggaaata aaattgcggt tttctgttag
     2281 tttactttct ataagagtct gttgttctgt taggttctca gaatttagca gtaataaggt
     2341 atcacccatt aatacagttt tattttcata taaattatac attgtaattt gaccatagac
     2401 agctgattgg attgttgtgt tttctgaagg tgtgcgtatt acgccacgac ttggtgtagt
     2461 aacgtcaatg tatacaaaag gcaacgatgc tattattaat agtactatcg ccactattat
     2521 accataaatg gctcggctac gtgtgtttat ctgggtatgg taactctcta cggtattttc
     2581 aatgatttcg gaaggaaaca gttgttgcat aattgtataa tgagctaaca gtagcttatg
     2641 tttttatatg ggttcattta tctcggtgtt gtattgaggg tt
//
LOCUS       DAOPUR010000198         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_39725_length_2680_cov_3.429714, whole genome shotgun sequence.
ACCESSION   DAOPUR010000198 DAOPUR010000000
VERSION     DAOPUR010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39725_length_2680_cov_3.429714"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /gene="gldL"
                     /locus_tag="PKN90_04275"
     CDS             complement(<1..258)
                     /gene="gldL"
                     /locus_tag="PKN90_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNZ85527.1"
                     /translation="MTAKAYGFGAALVIIGALFKIMHFPGAGIMLSIGMGVEALLFAM
                     SAFEEPHKEYDWSIVYPELDGGEAEERAPKVKAGAPLSVSGV"
     gene            complement(346..1758)
                     /locus_tag="PKN90_04280"
     CDS             complement(346..1758)
                     /locus_tag="PKN90_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ85528.1"
                     /translation="MKKLLILFAALAALVSCSNKGSGELVGVQGDAWKDPTPYGMVFI
                     PSGSFTMGPNDQDANWALSSQSKVVSVDAFWMDETEITNREYKQFVYWVRDSIVRTKL
                     GEVDEAFLVTQDKRGNELEKPRINWKPKIPWSKANEEQAEAIDAMFYADDESLALTKE
                     LNGHILHYKYAWIDYNQAALKRNKFNTNTGKFEADLSLLGKGKDSADVMVKKDTAYIT
                     EEGYIVNQTKFVELKNRRDFISSKIINVYPDTLSWVRDFTYAYNEPYMKMYFYHDGYG
                     DYPVVGINWEQANAFCHWRTNFYNKAQIDNKRHIVQDYRLPTESEWEYAARGGRDLSM
                     YPWGGNYIRTAKGCYMANFKPQRGNYTVDGHMVAAKVASFPPNNYGLYDMSGNVAEWT
                     SSAFHESNYSFVNDLNPSYQYNAKASDPEIMKRKVIRGGSWKDIGFYLQCGTRTYEYQ
                     TESRSYIGFRCVRSVIGANY"
     gene            complement(1779..>2680)
                     /locus_tag="PKN90_04285"
     CDS             complement(1779..>2680)
                     /locus_tag="PKN90_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNZ85529.1"
                     /translation="INGIAQQNMQFSLYTLNLNAINPASTGINEMIHISGVHRQQWLN
                     IKRAPQITYMNIELPFSIENSKHGAGIGFLNQGIGLFTNQSIVLKYAYHKKINEKTNL
                     SGGLNIQFNSIGFNIKDATTGTSDFHSPNDPAIPTGSEISDLKTDIGVGAMVKNETYL
                     LGVALQNIVSPTYQLSNTEYNTPMNINLVGIYNIKTISSLYLIQPSVLVKTDFATWQC
                     ELGSTIQYKEKYWGGLGYRFQDAIIFQVGMQLANGLTIGYAFDLPISSMIYTSVGSHE
                     LSLSYSFKIEFSKKQTYKSIRHL"
BASE COUNT          774 a          501 c          455 g          950 t
ORIGIN      
        1 aactccagat acgcttaatg gtgcaccagc ttttactttt ggagctcttt cttctgcttc
       61 tccaccatct aattctggat aaacgatact ccaatcatat tctttgtgtg gttcttcaaa
      121 tgcagacatt gcaaatagta atgcctcaac acccataccg attgataaca taataccagc
      181 accggggaag tgcataattt taaataaagc accgatgata actaacgcag caccaaagcc
      241 atacgccttt gccgtcattt ttttcccttg agggctactt aagaaatttc ctttagacat
      301 ttttgtgtga tttttaggtt aatagataat ttattattaa tttatttagt aatttgctcc
      361 gattacactt cttacacaac ggaatccaat atacgaacgt gattctgttt ggtattcata
      421 agttctagtg ccacattgta aatagaatcc tatgtctttc catgagccac cacgaataac
      481 tttacgtttc atgatttcag gatcgcttgc tttggcattg tattgataac ttggattcaa
      541 gtcgttcaca aaactataat tagattcgtg aaacgcagac gaagtccatt ctgcaacgtt
      601 gcctgacata tcatacaatc cgtaattatt aggagggaaa gaagctactt tagcagcgac
      661 catatgtcca tcaactgtgt aattacctcg ttgaggttta aagtttgcca tatagcaacc
      721 ttttgcagta cggatataat ttccacccca tgggtacata gataaatcgc gaccaccacg
      781 agcggcgtat tcccattcag actcagtagg gaggcggtaa tcttgtacaa tatgacgttt
      841 gttgtctatt tgtgctttat tgtagaaatt tgttctccaa tggcagaaag catttgcttg
      901 ctcccagttg atacctacaa caggatagtc tccataaccg tcgtgataga aatacatttt
      961 catgtatggc tcgttgtatg cataggtaaa gtcgcgtacc caagaaagag tatctggata
     1021 aacattaatt attttagaag aaataaaatc tcttctgttt tttaattcaa caaattttgt
     1081 ttgatttacg atataacctt cttctgtaat ataggcagta tcttttttta ccattacatc
     1141 agcactatct tttcctttac ctagtaagga taaatctgct tcaaatttac ctgtatttgt
     1201 gttaaacttg ttgcgtttta aggctgcttg attatagtca atccatgcat atttgtaatg
     1261 gaggatatgt ccattcaatt cttttgtaag agcaagtgat tcgtcatcag cataaaacat
     1321 tgcatctata gcttctgctt gttcttcatt tgcttttgac catggaattt ttggtttcca
     1381 gttaattcta ggtttttcta gttcatttcc tcttttgtct tgtgtaacaa ggaaagcttc
     1441 atctacttca ccaagtttag tacgtacgat tgagtcacgt acccaataca caaattgttt
     1501 gtactcacgg tttgtgattt cggtttcatc catccaaaaa gcatctactg agaccacttt
     1561 agattgagaa gaaagagccc agttcgcatc ttgatcgttg ggtcccatgg taaaggatcc
     1621 agatgggata aacaccatac cataaggggt ggggtctttc catgcatcac cttgtacacc
     1681 aactaattct ccacttcctt tgttactgca actaacaagg gctgcaagag cggcaaataa
     1741 aataagtaat tttttcatat ctaattttta ggttaagctt ataaatgtcg aatgcttttg
     1801 tatgtctgtt ttttcgaaaa ttcaatttta aaactatatg ataatgacaa ttcatgagat
     1861 ccaacactag tatatatcat ggatgaaatt ggtaaatcaa atgcatatcc tattgttagt
     1921 ccatttgcca attgcatacc tacttgaaaa ataatagcat cttgaaatct gtatcctaat
     1981 ccgccccagt atttttcttt gtattgtatg gttgacccta gttcacattg ccatgttgca
     2041 aaatcagttt tcactaaaac tgatggttgt attaggtata acgaacttat tgtttttata
     2101 ttgtatatcc caactaaatt tatgttcatt ggagtattat actctgtgtt gctcaattga
     2161 tatgttggag atacaatatt ctgtagtgct acgccaagca agtatgtttc atttttgacc
     2221 atggctccaa cgccgatatc tgtttttaaa tcagatattt cggaacctgt tggaatggca
     2281 ggatcatttg gcgaatgaaa atcacttgtt cctgtcgttg catcttttat attaaaccca
     2341 atactattaa attgtatatt caatcctcca ctgaggtttg ttttttcatt tatttttttg
     2401 tgatatgcat actttaagac aatagactgg tttgtaaaaa gaccaatccc ttgatttaaa
     2461 aatccgatac cagcaccatg tttggagttt tcaatggaaa agggtaactc aatattcata
     2521 tacgtaattt gaggtgctct tttgatgttc aaccactgtt gacggtgtac tccagatata
     2581 tgtatcatct cgtttattcc agtagatgct ggattaatgg cattcaaatt tagtgtgtaa
     2641 agactaaatt gcatattttg ttgggcaata ccattgatag
//
LOCUS       DAOPUR010000199         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_40208_length_2657_cov_3.526134, whole genome shotgun sequence.
ACCESSION   DAOPUR010000199 DAOPUR010000000
VERSION     DAOPUR010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40208_length_2657_cov_3.526134"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PKN90_04290"
     CDS             <1..1016
                     /locus_tag="PKN90_04290"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNZ85530.1"
                     /translation="IKADVTMYLKAIEIDKNWKLVAKTTSHGFQWIDTPKLNVIGVSV
                     PITPIATIALQQSEELITSQIDAILAQTINIKKYVAMAWKEAQKPMQVSPENDLWVRI
                     SPKNVYVSPFTTNGQKLYVAISIQAVIESFMGVQPVQNKLTVLPPFTVSANQPKDFNL
                     NIATDITYEKISELAKSQLQNKTFSEGNKSITITDLSVFGSEGKAIFVADVIGSIKGR
                     IYFTGTLGYNAETMSIEVLQPEFDIKTKNALVKSANWLLNGFIIKKITPYLTYPVQDE
                     LTTMKTEANKMLTNYKVYDGVDLTGKLHTISVQRIDLVPGAIRLHANVGGNIALKVND
                     IHF"
     gene            complement(1110..>2657)
                     /locus_tag="PKN90_04295"
     CDS             complement(1110..>2657)
                     /locus_tag="PKN90_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNZ85531.1"
                     /translation="LMGKNGAGKSTLLKILAGVREPSRGKVTTPKDYEIAYLPQHLMT
                     EDSRTVFEEASQAFSSVFEMEKKINDLNEQLAIRTDYESDSYMALIEEVSALSEKFYS
                     IEEINYDGEIEKILLGLGFSRTDFNRKTSEFSGGWRMRIELAKLLLRNPQAILLDEPT
                     NHLDIESIQWLENFLIQSDKAVIVISHDRAFVDTITNRTIEVTMGQIYDYKATYSHYL
                     ELRKERRQQQQKQYEDQQKMIAETTEFIERFKGTYSKTLQVQSRVKMLEKLQIVEVDE
                     EDTSALRLKFPPSPRSGNYPVIAENVSKQYDDKLIFKDVNVMIHRGEKIAFVGKNGEG
                     KSTLVKCIMGEIPFGGTLTLGHNVKIGYFAQNQASMLDENLTVFQTIDDIAVGDIRTK
                     IRDILGAFMFGREDIDKKVKVLSGGERTRLAMIKLLLEPVNLLILDEPTNHLDLKTKD
                     ILKQALIDFDGTLITVSHDRDFLDGLATKVYEFGNQKVIEHHEGIYEFLEKKKLTNLK
                     ELERNKK"
BASE COUNT          830 a          498 c          495 g          834 t
ORIGIN      
        1 taattaaagc agacgttacc atgtatttaa aagctattga gattgataaa aattggaaat
       61 tagtagccaa aacgacctcg catggttttc agtggattga tacaccaaaa ctgaatgtta
      121 taggagtaag tgtacctata acacccattg ctactattgc acttcagcaa agcgaagagt
      181 tgataacaag tcagattgat gctatattgg cacaaacgat aaatataaaa aaatatgttg
      241 ctatggcatg gaaagaagca caaaagccca tgcaagtaag tcccgaaaat gacctatggg
      301 tgcgtattag tcctaaaaat gtgtatgttt caccattcac aaccaatgga cagaaattat
      361 acgttgctat ttctatacaa gcagtaatag agtcatttat gggggtacaa cctgttcaaa
      421 ataagctcac agtgctaccg ccctttaccg tatcagcaaa tcagccaaag gattttaacc
      481 tgaatattgc tactgatata acgtacgaaa aaatttcaga attagctaaa tcgcagttgc
      541 aaaataaaac attttcggaa ggaaacaaaa gcattacaat aactgacctt tcggtatttg
      601 gtagcgaagg aaaagctatt tttgttgctg atgttattgg gtcgataaaa ggtcgtattt
      661 actttacggg aacgttagga tacaatgccg aaacaatgtc aattgaagta ttacaacctg
      721 agtttgatat taaaactaaa aatgctcttg taaaatcagc aaattggttg ttaaatggat
      781 tcattataaa aaaaataacg ccttatttga catatcctgt gcaggacgaa cttacaacaa
      841 tgaagacaga ggcgaacaag atgctcacaa actacaaggt gtatgatggt gtagacctta
      901 ctggcaagtt acacacaata tccgtgcaaa gaatcgattt agtgcctgga gctatacgtt
      961 tgcatgcgaa tgtaggtgga aatattgccc taaaagtaaa cgatatacac ttctaaatcg
     1021 ggagtgtaaa catataggca gtccaaacaa agagctgtaa atatataaag ttgagttatg
     1081 ccatagcaat agaacttggc tactagtagc tattttttat tacgctccaa ctcttttaag
     1141 ttagttagtt ttttcttttc aagaaattcg taaatacctt cgtggtgttc tataactttt
     1201 tgattaccaa attcatatac tttcgtagcc agaccatcta aaaaatcacg gtcgtgcgaa
     1261 accgttatta acgtgccatc aaagtcgatg agggcttgtt tcaatatatc ttttgtcttc
     1321 aagtcaagat ggttggtagg ctcatctaat atcagcaagt ttacaggttc gagcaacagt
     1381 tttatcatgg ctaatcgtgt ccgttcgcca cccgaaagca ctttaacttt tttgtcaata
     1441 tcctctctac caaacatgaa agctcccagt atatcgcgaa tctttgtacg tatatcgccc
     1501 acagcaatat catcaatggt ttgaaaaacg gtaagatttt cgtctaacat tgaggcttga
     1561 ttttgagcaa aatacccaat ttttacattg tgtccgaggg tgagtgtacc gccaaaagga
     1621 atttcgccca taatacattt taccaaggtt gatttacctt cgccattttt gcctacaaaa
     1681 gcaatttttt cgcctcggtg tatcattaca tttacatcct taaatatcag cttatcatca
     1741 tactgtttgc ttacattttc ggctattacg gggtaattac ccgaacgtgg cgagggagga
     1801 aatttgagtc gcaaagcaga tgtatcttct tcatcaactt ctacaatttg caatttttcg
     1861 agcattttta cacgcgattg cacttgcagg gttttggaat aggtgccttt aaaccgttcg
     1921 ataaactcgg tggtttcagc aatcattttt tgttggtcct catactgttt ttgctgttgc
     1981 tgacggcgtt ctttacgtag ttcaaggtag tgagagtagg tagctttgta atcatatatt
     2041 tgtcccatcg ttacctcaat tgtacgattg gtaatcgtat caacaaaagc cctatcatgc
     2101 gaaatgacaa taaccgcttt atcactttgt atcaaaaagt tttccaacca ttgaatggat
     2161 tcaatgtcga ggtggttggt aggttcgtcg agcaagatag cttgtggatt gcgtaataat
     2221 aacttcgcta gttcgatacg catacgccag ccaccgctaa attcactggt ttttctgtta
     2281 aaatcagtac gagaaaaccc taatccaagc aaaatctttt caatttcacc gtcatagttg
     2341 atttcttcga tgctataaaa tttttcactc aacgccgaaa cttcttcaat taacgccata
     2401 taactatcgc tttcgtaatc ggtacgtatg gcaagttgtt cgttgaggtc attaattttt
     2461 ttctccatct caaatacaga agaaaaggct tgagacgctt cttcaaatac tgttcgagag
     2521 tcttctgtca ttaagtgttg aggtaaatag gctatttcgt aatctttagg agtggttact
     2581 ttcccacgtg atggttcgcg aacacctgct aaaattttga gtaaggtaga tttaccagca
     2641 ccatttttac ccattag
//
LOCUS       DAOPUR010000200         2652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_40320_length_2652_cov_3.239892, whole genome shotgun sequence.
ACCESSION   DAOPUR010000200 DAOPUR010000000
VERSION     DAOPUR010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40320_length_2652_cov_3.239892"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKN90_04300"
     CDS             <1..389
                     /locus_tag="PKN90_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNZ85532.1"
                     /translation="GFKIAILSGGFTYFGNALKKQFDVDYVYANELEIVNGKLTGKHL
                     GEIVDGKRKAELLRLIAQFEKIELEQVIAVGDGANDLPMLNLAGLGIAFHAKPKVKEN
                     AQQAISTIGLDGILYFLGFRDIHMNE"
     gene            399..1130
                     /locus_tag="PKN90_04305"
     CDS             399..1130
                     /locus_tag="PKN90_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ85533.1"
                     /translation="MDNSKRLNKAISETGFCSRREADRLIAENRVQVNGIIGNVGMQV
                     TPHDSILIDGKKIIKDVTSIYIAFHKPPGITCTTDITVKDNIISFINYPERIFPIGRL
                     DKASEGLIFLTNDGDIVNKILRSKNNHEKEYIVRVNKPITNDFLQKMSTGVPILDTIT
                     KPCTVKKMNEHTFSIILTQGLNRQIRRMCEYLDYGVSTLKRIRIMNISLGNLKKGHLR
                     HFTNTELTELLALVEDSSKTEDASL"
     gene            1222..2496
                     /locus_tag="PKN90_04310"
     CDS             1222..2496
                     /locus_tag="PKN90_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85534.1"
                     /translation="MKSKYTSIFLLVFMLYACVPSSEKTYTYLVNEPIYMDINEFRNS
                     VAITQDTHPITSYGKISYYKGFLFVSEPQKGIHIINNTNPNNPYIVGYIELLGNADIA
                     IKNDILYADSYIDLVWFNITNPSIPVYEGRLENAFEEAFPPLEDVNLGIDGSMVWNKP
                     DDKIIVGWTITSRTETRSNSIFSGFPWWIETDPVPVMSEKNTNGINGSMSRFSLHDDY
                     LYAVINNNMSIINVSGTTPVKAVDNLYIGWNVETIFYYKNNMFMGTPTGLLIYSVENP
                     TQPLFKSSIQHVLGCDPVVVENDIAYVTIHSGNLCGQNNNDLIAIDVKDVENPKQVVS
                     YTMTKPKGLGIDNGTLFICDDGLKIYKADNPQTIMANKLAHYTNIDGYDVIPFENTLM
                     MIASDGIYQYDYSDLNNIHQISFLPMTNEKEE"
BASE COUNT          965 a          433 c          440 g          814 t
ORIGIN      
        1 atggttttaa aattgctatt ttatctggag gttttacata ttttgggaat gcattaaaaa
       61 aacagtttga tgtagattat gtatatgcca atgaattaga gattgtaaat ggtaaactga
      121 caggaaaaca tcttggagaa attgtggatg gaaaacgaaa agcagaatta ttgcgtctca
      181 ttgctcaatt tgaaaaaatc gaactcgaac aagtaatagc cgtgggcgat ggggcaaacg
      241 atttacctat gctgaattta gccggattag gcattgcttt tcatgcaaaa cctaaagtaa
      301 aagaaaatgc ccaacaagct atctcaacta ttggacttga tggcattctt tatttcttag
      361 gatttagaga tatacacatg aacgaataat ttcatggcat ggacaatagc aaacgactaa
      421 ataaagcaat atccgaaaca ggcttttgtt cgcgtagaga agcagataga ctaattgcag
      481 aaaatcgtgt tcaagtaaac ggaataatcg gcaacgttgg tatgcaagta actccacacg
      541 atagtatttt gattgatgga aaaaaaatca tcaaagatgt aacatcaatt tacattgctt
      601 ttcacaaacc tccagggatt acatgcacta ccgatataac cgtcaaggat aatattatta
      661 gttttatcaa ctatccagaa cgcattttcc caattggcag acttgacaaa gcgagcgaag
      721 gtttaatttt tttaactaac gatggtgata ttgtcaacaa aatattacgt tcaaaaaata
      781 accacgaaaa agaatatatt gtgagagtaa ataaacccat cacaaacgat tttttgcaaa
      841 aaatgagtac aggagtgccc atacttgata caattactaa accatgtact gttaagaaaa
      901 tgaatgaaca cacattttca attattctaa cacaaggact taacagacaa atacgtcgta
      961 tgtgcgaata tcttgattat ggggtaagta cattgaaacg aattcgtatt atgaatatat
     1021 ctcttggaaa ccttaaaaaa ggacatcttc gacactttac caacactgaa ttgactgaat
     1081 tattagcgtt agtagaagac tcttcaaaaa ccgaggatgc gtcattataa attagttgaa
     1141 aaaaattaac acagtaaaat atctgtttta cgtttttctg atatatattt gtgcataatt
     1201 actcatttta aataggatac tatgaagtca aagtatacaa gcatattctt attggtattt
     1261 atgctttacg cgtgtgttcc aagctctgaa aaaacatata cctatttagt aaacgaaccc
     1321 atctatatgg atatcaacga atttcgtaat tctgttgcca tcacacaaga tactcaccct
     1381 attacttcat atggtaaaat cagttactac aaagggtttt tatttgtatc agagcctcaa
     1441 aaaggtatac atataataaa caatacaaat cccaataatc catacatagt aggttatatt
     1501 gaactactgg gcaatgcaga tattgcaatt aaaaatgata tcttatacgc cgattcatac
     1561 attgatttag tttggtttaa tataacaaat ccatccatac ctgtgtatga gggtcggtta
     1621 gaaaatgcat ttgaagaagc gtttccacca ttagaagatg tcaaccttgg gatagatggc
     1681 tcaatggtat ggaataaacc cgatgataag attatagttg gttggacaat aacttcgcga
     1741 actgaaactc gttcaaactc tattttttca ggatttccct ggtggataga aacagaccca
     1801 gttcctgtta tgtccgaaaa aaatacaaac gggataaacg gatctatgtc cagatttagt
     1861 ttacacgatg attacctata tgctgttatt aacaacaaca tgagcatcat caatgtaagt
     1921 ggcacaacac ctgtaaaagc agttgacaat ctatacattg ggtggaatgt agaaacaata
     1981 ttctattata aaaataatat gtttatggga accccaacag gcttattaat ttattctgta
     2041 gaaaacccca ctcagccatt attcaaatca tcaatacaac atgttttggg ttgcgaccct
     2101 gtagtagttg aaaatgatat tgcttatgta acgattcact ctggtaattt gtgtggtcaa
     2161 aataataatg atttaattgc aattgatgta aaagatgtag aaaacccaaa acaagtagta
     2221 tcttatacga tgaccaaacc caaaggatta ggaattgata atggtacatt gtttatctgt
     2281 gatgatggat tgaaaatata caaagctgat aatcctcaaa caattatggc taataaatta
     2341 gcccattaca ccaatattga tggatatgat gttatccctt ttgaaaatac cttaatgatg
     2401 atagcctcag acggcattta ccaatacgat tattcagact tgaacaatat acatcaaatt
     2461 agttttttac ccatgactaa cgaaaaagaa gagtaaaaca taattacctc ttctttttac
     2521 aacatacaat gagactattc agatactctc attgtatgtg aaatcggggt ttactcaaat
     2581 tctaggtaaa taccttcaaa atcttttttt agctgttgtt ttttagcact actccacaaa
     2641 gtcatatcta at
//
LOCUS       DAOPUR010000201         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_40333_length_2651_cov_3.365177, whole genome shotgun sequence.
ACCESSION   DAOPUR010000201 DAOPUR010000000
VERSION     DAOPUR010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40333_length_2651_cov_3.365177"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKN90_04315"
     CDS             <1..476
                     /locus_tag="PKN90_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNZ85535.1"
                     /translation="HGMSQRGGDVQANLRISDKPIFSDLIPLQQADLILSLEPMEALR
                     YMPYVKEAGWIVTNETPFKNISNYPNEADIEDEIKKHKNHILINIDEIAKEVGSARTA
                     NMVLLGAALPFLSLPEEDIEDAISAIFEKKGEEIIKLNIKALRAGAEISRNILNK"
     gene            481..900
                     /locus_tag="PKN90_04320"
     CDS             481..900
                     /locus_tag="PKN90_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNZ85536.1"
                     /translation="MLKNILSVTGKPGLYKLLSGNKKIIIAESLIDGKKSPVHSHEKV
                     ISLADIAIYTDTKEVPLREVFATLLEKSEGKESLDSKVSSTDELKKHFATVLPDYDRD
                     RVHNSDIKKIYAWYNLLIKNNITDFSEEKENEEVVSE"
     gene            complement(970..1713)
                     /locus_tag="PKN90_04325"
     CDS             complement(970..1713)
                     /locus_tag="PKN90_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ85537.1"
                     /translation="MISLLKNRATVRQYSDKNISEELLIDLLEASFRSSNTGNMQVYS
                     VVVTRDEEMKKQLAPCHFNQPMIVQAPIVLTFCADFNRFTKWCEYNKAIPGYDNFLSF
                     ITATIDATIVAQTFAIAAESKGLGICYLGTTNYFAEKISNILHLPRFVVPITTITVGY
                     PASIPNQTDRLPTRSFVHYEQYTDYTEDAIRNMYAEKENLPEMKQFVLDNKKETLAQV
                     FTDVRYTKSNNETFSAMLLSFLQKQGFRV"
     gene            2034..>2651
                     /locus_tag="PKN90_04330"
     CDS             2034..>2651
                     /locus_tag="PKN90_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNZ85538.1"
                     /translation="MEQKTYTFDEALQASIPYFEGDELAAKVWINKYALKDSYGNIYE
                     KTPDDMHWRLANEIARIESKYPNALTADELFELMRNFKYIVPQGSPMTGIGNHHQIAS
                     LSNCFVIGFTGESDSYGAVIKIDEEQVQLMKRRGGVGHDLSHIRPNGSPVKNSALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLSVSIKHPDAESFIDAKLEQ"
BASE COUNT          969 a          408 c          445 g          829 t
ORIGIN      
        1 tacatggtat gagccagcgt ggtggagatg tacaagccaa tttacgtatt tcggataagc
       61 caattttctc agacttaata cctttgcaac aagctgacct tattttgtct ctcgaaccga
      121 tggaagcatt gcgttatatg ccttatgtaa aagaagctgg atggatagta actaatgaaa
      181 ctccattcaa aaatattagc aattatccaa acgaagctga tattgaagat gaaataaaga
      241 aacacaagaa tcatatttta attaatattg acgaaatagc aaaagaagta ggttctgcac
      301 gcactgcaaa tatggtttta ttaggagctg ctctcccatt tttatcttta ccagaagaag
      361 atatagaaga tgctatatct gccatttttg agaaaaaagg agaagaaatt attaaattaa
      421 acatcaaagc cttacgtgct ggagctgaaa taagtagaaa cattttaaat aaataataat
      481 atgctgaaaa atattttatc tgttactgga aaacctggtt tatataaatt attgtctgga
      541 aataaaaaaa taattatagc cgaatcatta attgatggta aaaaatcgcc tgtgcactcg
      601 cacgaaaaag taatttcgtt ggcagatatt gccatttata ctgatactaa agaagttccc
      661 ctgcgtgaag tgtttgctac gttgcttgaa aaaagtgaag ggaaagaaag cctcgactca
      721 aaggtatcta gcaccgatga actaaaaaaa cactttgcaa cagttttacc tgattatgat
      781 agagatagag tgcataatag tgatatcaaa aaaatatatg cctggtataa cctactaatc
      841 aaaaataata ttacagattt ctcggaagaa aaggaaaacg aagaagttgt atctgaataa
      901 atatgtttca tcgtttaggt tatagctaag gagcaaagac gtaaacgatg aaaattaaaa
      961 cagattgtgt tataccctaa aaccttgttt ttgaaggaaa ctcaataaca ttgcagaaaa
     1021 tgtttcgtta ttactttttg tataacgaac atccgtaaat acttgagcca gtgtttcttt
     1081 tttgttatct aaaacaaatt gtttcatttc aggcaggttt tctttttctg catacatgtt
     1141 tctaatagca tcttctgtat aatcagtata ctgttcgtaa tgcacgaatg atctcgttgg
     1201 taatctatct gtttggtttg gtatagaagc tggataacct actgtaatag ttgtaatagg
     1261 cacaacaaaa cgaggtaaat gtaatatatt gctaattttt tcggcaaaat aattggtagt
     1321 acctaagtaa catatgccca agccttttga ctctgcagca atagcaaagg tttgagcaac
     1381 aatggtagca tcaattgtgg ctgtaataaa tgacaagaaa ttatcatacc ctggaatggc
     1441 tttgttgtat tcacaccatt tagtaaaccg gttaaaatcg gcacaaaaag ttaaaactat
     1501 cggagcttgt acaatcatgg gttgattaaa atgacatgga gctaattgtt ttttcatttc
     1561 ttcatcgcgt gttacaacca cactatatac ttgcatattg cccgtatttg aacttctaaa
     1621 tgaagcttct aataaatcta ttaacaattc ctcacttatg tttttatccg aatactgacg
     1681 aacagtagct ctatttttaa gtaaactaat catatggtat atatgtattt attaatttac
     1741 aaaaatactc aatttctctc aataaacaaa ttaaataaat tacgaatatt aaattaatgt
     1801 gatttttttt tgacaactat ttcatccaaa aatattaata aaatactaat aatcatgtat
     1861 ttataaaata tttttgacaa ctttacaaaa aagaaaaata aatttactgc aattatttta
     1921 catttttatt tataaaaaac tttgttaagt aaaaaaaata gtgtaacatt gtagtcccgt
     1981 gaagcacgaa accaactttt tataatcgac ataaaatcaa tattttagaa aaaatggaac
     2041 aaaaaacgta cacatttgat gaggcattgc aagcatcaat cccttatttt gaaggagatg
     2101 agttagcagc aaaagtatgg ataaacaaat acgcactaaa agattcttac gggaatattt
     2161 acgaaaaaac acctgatgat atgcattggc gtttggcaaa tgaaatagca cgtattgaat
     2221 caaaatatcc aaatgcatta acagctgatg aattgtttga attaatgcga aatttcaagt
     2281 acattgttcc acaaggaagc cccatgacag gtattggcaa tcatcaccaa atagcatcct
     2341 tatccaattg ttttgttatt ggttttactg gcgagtccga ttcgtatgga gctgtaataa
     2401 aaatagatga agaacaagtg caattgatga aacgtagagg tggcgttggg catgatttgt
     2461 ctcatattcg cccaaatggt tctccagtaa aaaattcagc acttacatca acaggtattg
     2521 ttccgtttat ggaacgctat tcaaactcta cacgcgaagt agcccaagac ggcagaagag
     2581 gtgcattaat gttaagtgta tcaattaaac accccgatgc agagtcattt attgatgcaa
     2641 agctagagca a
//
LOCUS       DAOPUR010000202         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_40689_length_2635_cov_2.538760, whole genome shotgun sequence.
ACCESSION   DAOPUR010000202 DAOPUR010000000
VERSION     DAOPUR010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40689_length_2635_cov_2.538760"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKN90_04335"
     CDS             <1..401
                     /locus_tag="PKN90_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85539.1"
                     /translation="LMLEDTPTILAHADLIKNGFQDSGIQQEKWYQQALFDMIDVAAE
                     RDVIIEINTRGLYKQRTSELYPCEQALRYMKQKGVRMMLSADTHLLTELTSGFDYAVG
                     VLQNNGINEITVFRKGQFVEIPFSSTGFDY"
     gene            complement(597..2066)
                     /locus_tag="PKN90_04340"
     CDS             complement(597..2066)
                     /locus_tag="PKN90_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNZ85540.1"
                     /translation="MNTQFNFRLVVRVIGALLLVESIILLLVSLVPIFYNEGDAFYFI
                     VSAIISFIIGIILFGLGHHHADKIGKREGSIIVTFTWIFFSLIGLLPYWLSNSIPSFT
                     NAFFETISGFTTTGASILNNIEELSHGMLFWRSLTHWIGGIGIIVISLAIMPVFSTSG
                     TQLFAAETSGPTKDKIHPKIHGTAKRLVLIYVILTFLETILLRFGGMGWFDAICHSFA
                     TIATGGFSTKQSSIAYWDSAYIQYIIILFMILSGINFSLYYFGFKKRFDKILKNEELR
                     YFLLVLISFTLIITFSLFTFSTDTTWQVIEQAFRDALFTVSSLMTTTGFGTADYVLWA
                     PFTWIILLIVMLTGASAGSTSGGIKMIRIVITIKACFYEFKRILHPQAIVPVRYNNLL
                     VSESILNKVLGFLFLYLIVALAGSLILAASGMNFEESIGGMVSCLGGVGPGLGSVGPY
                     GNYAAISDSSKWILSVAMLAGRLELFTFFLIFTPTFWKK"
     gene            complement(2302..>2635)
                     /locus_tag="PKN90_04345"
     CDS             complement(2302..>2635)
                     /locus_tag="PKN90_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ85541.1"
                     /translation="VSSYNNNGDEETEIQTYYYSSGNNTTHIMENDTDAFVKVYYNPT
                     NEYIEVVSDAENNIRFYLYDSKGSMITQTTLQEGSGKISISQLSPGTYLYTLLNNNEP
                     LRGKLIKQ"
BASE COUNT          916 a          456 c          449 g          814 t
ORIGIN      
        1 aactaatgct cgaggataca ccaacaattt tagcacatgc cgacttgatt aagaatgggt
       61 ttcaagacag tggtattcaa caagaaaaat ggtatcaaca ggctttgttc gacatgattg
      121 atgtggcggc tgaacgagat gtaattatcg aaattaatac gcgaggatta tacaaacaac
      181 gtacttcaga actatacccc tgcgaacagg cattgcggta tatgaaacaa aaaggggtga
      241 gaatgatgct cagtgccgat actcatttac ttacagagct aacttcgggt tttgactatg
      301 ccgtaggagt actacaaaat aatgggatca acgagattac tgtatttcgt aaagggcagt
      361 ttgtggaaat tcctttttct tctaccgggt ttgattattg atggtataaa aacacaaaag
      421 gcagtctcgc ctgactgcct acttgctaac cttaaaaatc aaatactatg aaaaattcat
      481 aagcaaaaat actatgattt tgtagtttct ggcaatacga gcttactcgt tgtctataaa
      541 ttaacagatt tttatagata ttttacaaag ctgttacaat ttgacgtgat gtagacctat
      601 tttttccaaa aggtaggtgt gaaaattaag aaaaaggtaa atagttcaag tctcccagcc
      661 agcattgcta ccgataatat ccatttactg ctatctgaaa tagcggcgta attaccatat
      721 ggtccaacag agcctaaccc tggtccaact cctcccaagc aagaaaccat accaccaata
      781 gattcttcaa aattcatgcc tgaagcagcc aatataagtg aacccgctag tgccactata
      841 aggtataaaa ataaaaatcc taatacttta ttcaggatac tttcacttac tagcaaatta
      901 ttataccgaa caggtacaat tgcttgagga tgtagtattc ttttaaactc gtagaaacaa
      961 gcttttatgg taatcacaat gcgtatcatt ttaataccac cacttgtcga accagccgaa
     1021 gctcctgtaa gcattacaat gagtaaaata atccatgtaa aaggagccca taaaacataa
     1081 tctgcggtgc caaaacctgt agtagtcatt aaacttgata ccgtaaacaa tgcatctcta
     1141 aatgcttgtt caataacttg ccatgttgta tctgtggaga aagtaaataa tgaaaacgta
     1201 ataataaggg taaagcttat taatacaagt aaaaagtaac gtaattcttc attttttaga
     1261 attttatcaa atcgtttttt aaaaccaaag taatataaac tgaagttaat gcctgataat
     1321 atcataaaaa gaataataat gtactggata taggcggagt cccagtaggc tatgcttgat
     1381 tgttttgtgg agaatccccc tgtagcaata gttgcgaacg aatgacagat tgcatcaaac
     1441 cagcccatac ctccaaaacg caacagtatc gtttctaaaa atgtaagtat tacataaatt
     1501 aaaactaaac gctttgctgt tccatgtatt ttgggatgta tcttgtcttt tgttggtcca
     1561 gaagtttctg ccgcaaataa ttgtgtgcca cttgtactaa atacaggcat gattgccaac
     1621 gaaataacga taatgccaat accaccaatc cagtgagtta aacttcgcca aaataacatc
     1681 ccatgagata attcttcaat attatttaaa atggaagctc ctgtagttgt aaaacccgaa
     1741 attgtttcaa aaaaagcgtt tgtaaatgaa ggaatggagt tgcttaacca ataagggagt
     1801 aatccaatta aggaaaaaaa tatccatgtg aaagttacaa taatggatcc ttctctcttg
     1861 cctattttgt cagcatgatg atgccctaac ccaaataata taatcccaat aataaaacta
     1921 ataatggcag atacaatgaa ataaaaagca tctccttcat tatagaagat gggaacaaga
     1981 cttactagaa gtaaaattat agattctacc aacaataatg ctccaataac tcgcacaacc
     2041 aatcgaaaat taaattgagt attcattatc caacgattaa taaagagtgt ttattttacc
     2101 attttttttt gacaaaaaag ggtctttgga aatgctagag agaatgcaca atgtatttct
     2161 ctgagagcat aaagaaaggc tgacaaagat attcgtttta cattatctgt acaataaaaa
     2221 ttgaaattaa tttggtcttt tgacaaaaag aaaaacaagc atgctagtat gcctgttttt
     2281 cttgtataaa ttacagtgtt tttactgttt tatcaattta ccacgtaatg gttcgttgtt
     2341 gttgagtaag gtatatagat acgttcccgg tgataattga gatatagata ttttccctga
     2401 accttcttgt aaagtcgttt gagtaatcat agatcctttg ctatcgtata agtagaatcg
     2461 tatattgttt tcagcatcac ttaccacttc tatatactca ttagttggat tgtaatagac
     2521 ttttacgaat gcgtctgtat cgttttccat gatgtgtgtt gtgttgttgc cagacgaata
     2581 ataatacgtt tgaatctcag tttcctcgtc tccattgtta ttataggatg atact
//
LOCUS       DAOPUR010000203         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_40866_length_2626_cov_2.941657, whole genome shotgun sequence.
ACCESSION   DAOPUR010000203 DAOPUR010000000
VERSION     DAOPUR010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40866_length_2626_cov_2.941657"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="PKN90_04350"
     CDS             <1..1680
                     /locus_tag="PKN90_04350"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNZ85542.1"
                     /translation="TIATSWLGNLGLPAESKIYFDKNQGYVTHSFLFKNTYASYFLSP
                     ENSSFYNTKTPFSNVSYHTGGFAYREEDNISGLFTVNATPKLNFSLLANHIYGRGAYQ
                     NQATDGLSGALYGSYLGDKYGIHFITALNNLKHFENGGIADISQLTSTIESYNLPVNL
                     SNAWSIFHSFYLYASQQYHLGFHTNDNKFVPVTTFGHSISFEQNQKKYYEKSISDFYQ
                     NSYYSTTSTNDTVAFNVLKNTAYISLNEGFHKWALMGVRAFIDFDIEQNTDLTADWSL
                     RKKTTSLVYAGGDISKTKGNTTYHALGKMMLLGDARGEYELNGKLKTQLKLGSQNIQL
                     QGKALLVSSNPYHHLEHYYSNHFMWERDYENIVRTQCSGSISLPNKVANITLKAGVEN
                     TTNYIYFDTSALPQQYSNAIQIVHGEVTLNLHLGNIHFENTAIFQQSSNEAILPLPSL
                     SSYHNLYVTHTLFNVLKFQIGADCRYHTAYYANAYMPATGQFYRQTETLIGNYPFINL
                     YANFHLKRTRFFVMYYNLSEAIFTDKNYTLVPNYPLNPGMVKFGLSWNFYD"
     gene            1726..2547
                     /locus_tag="PKN90_04355"
     CDS             1726..2547
                     /locus_tag="PKN90_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNZ85543.1"
                     /translation="MKLCSKISLVFVILVISFTVLFAFLRHSSPLFSTDYKDIIASGT
                     LRVVIEKNTMSYVVVNDTNVMGLQYKMVEQLAKDWGVTVQYIDASDLGSAINMLNNNE
                     VDIVVWHIPIHVEMEDKIAFTVPVFTSRQVLVQRKNTPHTLIRNQLDLARKTIHIPKD
                     SPYKQRIQNLAKEIGDSIFIVEINEISIDSLFYKVANAEIDFTVCEKVVASIEKKKYP
                     TLDTETAIGFKQNYAWAIRQNSSELQDSVNNWLNYFLETKEYNRIYQKYTNSRKQ"
BASE COUNT          952 a          485 c          426 g          763 t
ORIGIN      
        1 actatagcaa catcgtggct aggaaactta ggactacctg ctgaatcaaa aatttatttt
       61 gacaaaaatc aaggctacgt aacccattca tttttgttta aaaatacgta tgcatcctat
      121 tttctttcgc ccgaaaatag ttcattttac aatacaaaaa caccttttag taatgtaagc
      181 tatcatacag gaggttttgc ttacagagaa gaagacaata tttcgggatt atttacagta
      241 aacgctacac ctaaacttaa tttttcgtta ctagccaacc atatatacgg acgaggagcc
      301 tatcaaaacc aagcaacaga cggacttagc ggagcattgt atggtagcta tttaggagac
      361 aaatatggca tacattttat cacagcactt aacaacctaa aacattttga aaatggaggt
      421 attgctgaca taagccaact aacgagtacg atagaaagtt ataatctacc tgtcaattta
      481 tccaatgcat ggtctatttt ccattcattt tatttgtatg catcacagca ataccatctt
      541 ggatttcaca ctaacgacaa caaatttgtt cctgttacta cctttggtca tagtatatcg
      601 tttgaacaaa accaaaagaa atactacgaa aaaagcatat ctgattttta ccaaaactca
      661 tactacagta ccactagcac caacgacact gtggctttca atgtattaaa aaatacagca
      721 tatatatctc taaatgaggg ttttcataag tgggcattaa tgggagtacg tgcttttatt
      781 gatttcgata tagaacaaaa tactgactta acagctgatt ggagtttacg caaaaaaact
      841 acgagcctag tatatgcagg aggagacatt tcaaaaacaa aaggcaacac aacctatcat
      901 gctttaggca aaatgatgct attgggcgat gcacgtggag aatatgaatt aaatggcaaa
      961 ttgaaaacac aattgaaatt gggttcgcaa aacatacagt tacaaggcaa ggctctttta
     1021 gtaagcagta atccgtacca tcatcttgag cactattatt ccaatcattt tatgtgggaa
     1081 agggattatg aaaatattgt tcgcacccaa tgtagtggta gcattagttt gccaaacaaa
     1141 gtagcaaata taacactcaa agcgggagta gaaaatacca ctaattacat ctattttgac
     1201 accagtgctt tgcctcaaca atacagcaat gccatacaaa ttgtgcatgg cgaggtaaca
     1261 ctaaacctgc atctaggcaa tattcatttc gaaaatactg ctatttttca acaaagttca
     1321 aatgaggcta ttttaccctt accaagccta tcttcttatc ataatctgta tgttacacat
     1381 acattattca acgtactcaa atttcaaata ggagcagatt gtcgttatca cacagcttat
     1441 tatgccaatg catatatgcc tgctactggg caattttacc gccaaacaga gacccttata
     1501 ggtaactacc catttataaa cctatatgcc aactttcatc tcaaacgaac acgctttttt
     1561 gtaatgtact acaatctttc ggaagcaatt tttacggaca aaaattacac cttagtacct
     1621 aattacccat taaaccctgg aatggttaaa tttggcttat cgtggaattt ttatgattaa
     1681 tttgagtatc tttgtgtata acttacttaa aaaaacaaat gccctatgaa attatgttca
     1741 aaaatatcat tggtattcgt cattctagtt atttctttta cagtactttt tgctttcttg
     1801 cgacactcat ctccgctatt ctcaacagac tataaagata tcatagcatc aggcaccctg
     1861 agggtagtga tagaaaaaaa cacaatgagc tatgtggtgg taaacgatac caacgtaatg
     1921 ggtttgcaat acaaaatggt agaacaatta gccaaagatt ggggggtaac tgtgcaatac
     1981 atagacgcct ctgacttagg ttcggcgatt aatatgctca ataacaacga agtagatatc
     2041 gtagtatggc atattccaat acatgtagaa atggaagata aaattgcatt tacagtacct
     2101 gtttttacaa gcagacaagt attggttcag cgaaaaaaca cgccacatac acttattcgt
     2161 aaccaattag acttagcaag aaaaaccata cacatcccca aagattcccc ctataaacaa
     2221 cgtattcaaa atttggcaaa agaaattggg gacagcattt ttatagtaga aataaatgaa
     2281 ataagcattg attcgctgtt ttataaagta gccaatgcgg aaattgattt tactgtatgc
     2341 gaaaaagtag ttgcatctat tgagaaaaaa aaatacccca cactagatac cgaaacagct
     2401 atcggattta agcaaaatta tgcatgggct atacgtcaaa actcatcaga gctacaagat
     2461 agtgtaaata attggctaaa ctatttttta gagacaaaag aatacaaccg tatttaccaa
     2521 aaatatacaa atagtagaaa acaatgaata tagttgacca acgccgaaca tattaaacat
     2581 aattatccta cattaaaaat aagaaatggg ctgttttggg gagatg
//
LOCUS       DAOPUR010000204         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_40889_length_2625_cov_2.589494, whole genome shotgun sequence.
ACCESSION   DAOPUR010000204 DAOPUR010000000
VERSION     DAOPUR010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40889_length_2625_cov_2.589494"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..791)
                     /locus_tag="PKN90_04360"
     CDS             complement(162..791)
                     /locus_tag="PKN90_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNZ85544.1"
                     /translation="MKKVMLLVLGFLLGLNIIAQNEKPFYLGLYASPSVLWLQSDNNT
                     VDASGSKLGFSYGLLADITLGDYYALASGLEITQLGGTLSYQTGLAPVQQTVDYKLQY
                     LEIPLALKMQTASINNLSFFGRFGGGFGVRLDAKKDVNNTIINQPASDEIHLLRAYFS
                     VGGGVEYTVAYNTKIFAGIFYKNGLTNAFKQEDWSVKSNALSLNVGVIF"
     gene            1145..2284
                     /locus_tag="PKN90_04365"
     CDS             1145..2284
                     /locus_tag="PKN90_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85545.1"
                     /translation="MNLTSKLTQTNVTELSGLHWNPATQCLYVVQDNGRLRILQFNEG
                     ADDFFEIANVEIAGNPEGIMQVDYEASMFYTVDEKNYEIRQYVPNQDYTSITLMKKWR
                     LLTPNTPMPDTGNDGIEGIVFVPDKHLQSIGFVSSKTGLPYTSTKGMGGLIFIAHQTE
                     GYVWVYDVNPALSFDVAYVGKYKTNRKESCDIAFDRTTGLLYILHNVGKNFLEVTNLS
                     LEQVNDEYKFVVSKEYSLSNPTDGNENIEGFAITPMCEENGVVSVFVCRDVNDTGTEI
                     QKGDALRWFTDFPLLGSCLPITEDEQIQTDNARFTIEFTSFNGLYIHGAQENVELVFM
                     DELGRIQYKTHYEPMSYLPYDVLPQGYSILQIHTSNDIQVVLIRL"
     gene            2326..>2625
                     /locus_tag="PKN90_04370"
     CDS             2326..>2625
                     /locus_tag="PKN90_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85546.1"
                     /translation="MRILIVSLMLTHVMLATVHIHYLQKSVYETGRQQWGDWQNEANA
                     FCIDSTTLSIVNLSTKSETVYVRQGEAIGGYNERGNYYRLFEAQNEKGQKCLFFLY"
BASE COUNT          862 a          395 c          467 g          901 t
ORIGIN      
        1 aattgtagta aattatggtt atagaacccc cgcttccgcg aaagtccttt gaaagaagga
       61 ctttttttat gtttacctac ttttttcata tctacaaata aataagaacg gaatttagtt
      121 aaaccgttct tatgtgtatc cattttataa taaagagttc gttaaaaaat aacaccaaca
      181 ttcaatgata aggcattgct ttttacactc caatcttctt gtttaaaagc atttgttaat
      241 ccatttttgt agaaaatacc tgcgaaaatt ttagtgttgt atgcaacagt atattctaca
      301 cctccaccaa cgctaaaata ggcacgtaag agatgtattt catcacttgc aggttggttg
      361 ataatggtgt tatttacatc ttttttggca tctagacgta ctccaaaccc accaccaaaa
      421 cgaccaaaaa aacttaaatt attgatggat gcagtttgca tttttagggc taagggtatt
      481 tccaaatatt gtaatttgta atcaactgtt tgttgcacag gagctaaccc tgtttggtac
      541 gaaagagttc ctcctagttg agttatttct aaaccactag caagtgcata ataatctcct
      601 aaggtgatat ctgcaagcaa tccgtatgaa aatccgagct ttgacccact agcatccacc
      661 gtgttattat ctgattgtaa ccataaaaca gaaggggatg catatagccc taaataaaat
      721 ggtttttcgt tttgagctat gatatttaat cccaataaga aacctagtac aagtagcatt
      781 acttttttca tatctgtatc tttttgttga aaacaataat tatcaatata caaatatact
      841 aaaaaataca gtaatacaag gataagtggt ctttttttat attttctttt aagctattta
      901 gataggatat atcttgcttt attaggcgaa tatttctttt ttcttgaatt attctatatt
      961 tatatcttat ctttgctatt atcttaacta ttcttattca attaattaat ctgatatgtt
     1021 atccttgcct caatatagac aaacccataa agttgttttc tacgccttaa tgcacatact
     1081 ttttttacca tttcctttgt tttctcaaac gttgaattgg ccagctgagt catggagtag
     1141 tgcgatgaat ttaacatcta agttaacaca aacgaatgta acagagttga gtggcttaca
     1201 ttggaatcct gctacacaat gtttgtatgt agtacaagat aatggacgct tacggattct
     1261 tcaattcaac gaaggagctg atgatttttt tgagattgct aatgttgaaa tagcaggaaa
     1321 tcctgaaggg ataatgcagg tagattatga ggcaagtatg ttttatacag ttgatgaaaa
     1381 aaattatgaa atacgacaat atgttcctaa tcaagattat acttctatta ctttaatgaa
     1441 aaaatggcga ttgttaaccc ccaatacgcc catgcctgat actggaaacg atggtataga
     1501 aggaattgta tttgtgccag ataagcattt gcaatcgatt ggatttgtaa gctctaaaac
     1561 gggcttgccg tacacttcta ctaaaggtat gggcggatta atttttattg cacatcaaac
     1621 ggaaggttat gtatgggtat atgatgttaa tccagccttg tcttttgatg tagcgtatgt
     1681 gggcaaatac aaaacaaacc gtaaggagag ttgtgatatt gcttttgatc gtaccactgg
     1741 attgttatat atattacata atgtagggaa gaattttttg gaagttacca atttatctct
     1801 tgaacaggta aacgatgagt ataaatttgt cgtatcaaag gaatattcac tatcgaaccc
     1861 cacggatggg aatgaaaata ttgaaggttt tgcaattact cccatgtgtg aagaaaatgg
     1921 agtggtttcg gtgtttgtat gtcgagatgt gaatgataca ggtacagaaa tccaaaaggg
     1981 agatgcctta cgatggttta cggattttcc tttattagga agttgtcttc caataacaga
     2041 agatgagcaa attcaaacag ataatgcgag attcactatt gaatttactt catttaatgg
     2101 cttatacata catggtgccc aagaaaatgt agagttagtt tttatggatg aattaggaag
     2161 aatacaatat aaaactcact atgaacctat gtcttatttg ccatatgatg tattacccca
     2221 ggggtatagt atattgcaaa tacatacatc aaatgatatt caagttgttt tgattcgatt
     2281 gtagttgttt gtttaattat aaaaatgata tatatgaaaa agaaaatgag aatattaatt
     2341 gttagtctta tgttgactca tgtaatgttg gcaactgttc atattcatta tctacaaaaa
     2401 tctgtctatg aaaccggtag gcaacaatgg ggtgattggc aaaatgaagc caatgcattt
     2461 tgtattgatt caacaactct cagtattgtt aacctttcta ctaagtcaga aacagtatat
     2521 gttcgtcaag gtgaagcaat aggtggatat aatgaacgag gaaactatta tcgtttattt
     2581 gaagctcaaa atgaaaaagg acagaaatgt ctgttttttt tgtat
//
LOCUS       DAOPUR010000205         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_40891_length_2625_cov_2.363424, whole genome shotgun sequence.
ACCESSION   DAOPUR010000205 DAOPUR010000000
VERSION     DAOPUR010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40891_length_2625_cov_2.363424"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="PKN90_04375"
     CDS             <1..1467
                     /locus_tag="PKN90_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNZ85547.1"
                     /translation="IKGRTKSIYSIWNKIKKQDTPFENIYDLFAIRIILNSDITKEKA
                     DCWQVYSIVADMYQPNPKRLRDWLSIPKSNGYESLHTTVMGPQGKWVEVQIRTKRMDE
                     IAEKGLAAHWKYKGIQGESGMDEWLKNIREILENPELNAIDFMDEFKLNLYDKEVFVF
                     TPNGDLQKLPKGATLLDFAFHIHTGLGTKCVGGKANGKNVTLKYVLKNGDQIEIQTSA
                     TQKPKLEWLNIVTTSKAKNKIRQAVKDEQFKDAQMGREILQRRFKNWKIEVEESKISR
                     LAKKKGFKTVSDFYQELAQEKLDILSLRDAYIDLDKRETTEPSDYRSAENFVQDNTLQ
                     DTTSKEDVLIIDQNLRGIEYSLAKCCNPIFGDDVFGFITTSGGFKIHKKDCPNAPQLI
                     SRFGYRIIPARWSGKSGTSQYAITLGVIGVDDIGIVTNISSLLSKEKNITLRSISVNS
                     HDGLFEGRITIMVGELQELENIIKKIKTVKGVKSVQRI"
     gene            1521..2177
                     /locus_tag="PKN90_04380"
     CDS             1521..2177
                     /locus_tag="PKN90_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNZ85548.1"
                     /translation="MLHTNPCNKTILFDFDGTLANTLKTVVEIYNKVAPEYNCKTVEL
                     EDVSRLQAMTISHLMKEQGISHITLAILLVRVRKELHINIDHVKPFFGIEEQLQTLKK
                     MGYQLGIMSSNSQKNVHTFLESNNMKHLFDFVHTSKNIFGKHTAIKRIISNLSLQTND
                     VVYVGDETRDIEACKKIGVKIAAVSWGYNLPEVLQAMQPDVLIDEPHSLANCISKLAM
                     "
     gene            complement(2182..>2625)
                     /locus_tag="PKN90_04385"
     CDS             complement(2182..>2625)
                     /locus_tag="PKN90_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNZ85549.1"
                     /translation="IHGNKSQNARQNALRNFKDRTTRVLVATDIAARGIDIDALNLVI
                     NFEIPNIPETYVHRIGRTGRAGLDGKAVSFCDVEEIPYLRDIHKLIGMQIPVVSDHPF
                     PSSNVLTEVADNKNRAKKKPTIQPSKGKRRPFVSNKPTLVKQPRG"
BASE COUNT          954 a          450 c          484 g          737 t
ORIGIN      
        1 ataaaaggaa gaacaaaatc tatttacagt atttggaata aaataaaaaa acaagatacg
       61 ccttttgaaa acatttatga tttgtttgct attcgtatta ttctcaacag cgatataacc
      121 aaagaaaaag ccgattgttg gcaagtatat tcaatagtag cagacatgta ccaacccaat
      181 ccaaaacgtt tacgcgactg gctatccata cccaaaagca atgggtacga atcgctacat
      241 actacggtaa tgggaccaca aggcaaatgg gtagaagtac aaatacgtac caaacgcatg
      301 gatgaaatag ctgaaaaagg tttagcagca cactggaaat acaagggtat acaaggagaa
      361 tcggggatgg acgagtggct gaaaaacatt cgtgaaatat tggaaaatcc tgaattgaat
      421 gcgattgact ttatggatga gtttaagctc aacttatacg acaaagaagt atttgtattt
      481 acacccaatg gcgatttgca aaaactaccg aagggagcta ctctacttga ctttgccttt
      541 catattcata cggggttggg aacaaaatgt gtgggaggaa aagcgaatgg aaaaaatgtt
      601 actttaaaat atgtactaaa aaatggagac caaatagaaa tacaaacctc agctactcaa
      661 aaaccaaaat tagaatggtt gaatatcgta accacctcta aggcaaaaaa taaaatccgt
      721 caagctgtta aagacgaaca gtttaaagac gctcagatgg gtagagaaat tctgcaacgc
      781 aggtttaaga attggaaaat tgaagtagaa gaatcaaaaa tatcgcgttt ggctaagaaa
      841 aaagggttta aaacagtaag tgatttttac caagaattag cacaagaaaa attggacatc
      901 ctaagcctac gtgatgctta tatagactta gataaaaggg aaacgacaga accttcagat
      961 tatcgaagtg ctgaaaattt tgtacaagat aatacattgc aagatactac tagtaaagaa
     1021 gatgtattga taattgacca aaacttgcgt ggaatcgaat attcattagc aaaatgttgt
     1081 aacccaattt ttggcgatga cgtgtttggc tttattacga ctagtggtgg ttttaaaatt
     1141 cataagaaag actgtccaaa tgcacctcaa ctcatttcac gttttggata cagaattatc
     1201 ccagctcgtt ggtcaggaaa atcgggtact tcgcaatatg ccattacact cggagtaatc
     1261 ggggttgacg atattggtat tgtaaccaac ataagttcgt tgttatcaaa agaaaaaaat
     1321 attaccttac gttctatttc ggtaaattcg catgatggac tttttgaggg aagaattacc
     1381 attatggtag gagagttaca agaattagag aatattataa aaaaaataaa aacagtaaaa
     1441 ggcgttaaaa gtgttcaacg tatataaaac aggcattttt tattccctga caattaattt
     1501 ttatacaatt tattcatatt atgcttcata caaatccttg caataaaacc attttgttcg
     1561 attttgacgg aaccttggca aacaccctga aaacagtagt agaaatttat aacaaagttg
     1621 ctcccgaata caattgtaaa acggtagaat tagaagatgt gtcgaggcta caagcgatga
     1681 caatatcgca tttaatgaaa gaacaaggca tctcacatat cactttagct attttgttgg
     1741 tacgagtacg caaggaatta catatcaaca tcgaccacgt gaaaccattc tttggcatcg
     1801 aagaacaact acaaacatta aaaaaaatgg gttatcaatt aggaattatg tcttcaaact
     1861 ctcaaaaaaa tgttcacact tttttagaat caaacaacat gaaacaccta tttgatttcg
     1921 ttcacacaag taaaaatatt tttggcaaac acactgctat taaacgtatt atatcaaact
     1981 tatcgctaca aacaaatgat gtagtatatg taggtgacga aactcgcgac atagaagctt
     2041 gcaaaaaaat tggtgtaaaa atagcggcag ttagttgggg ctataattta cccgaagtat
     2101 tacaagctat gcaacctgat gtacttattg acgaaccaca tagcctagca aactgtatat
     2161 ctaaactcgc tatgtaatgc cttatcctct gggctgttta accaaagtag gtttattgct
     2221 tacaaatgga cgccttttgc cttttgaagg ctgtatggta ggtttctttt tcgctcgatt
     2281 tttgttatca gcaacttctg ttaatacatt gctactagga aaaggatgat ccgacacaac
     2341 gggtatttgc atgcctatta acttatgaat atcacgcaag taaggaattt cttccacatc
     2401 gcaaaaagaa accgctttgc catctaaccc tgccctacct gtacgcccaa tacgatgcac
     2461 ataggtttcg ggtatatttg gaatttcaaa attgataacc aaattaaggg catcaatatc
     2521 aatgccacgt gccgcaatat cagtagctac taatacacgc gtagttctgt ctttaaaatt
     2581 tcgcaaggca ttctgacgtg cattttgcga tttattgcca tgaat
//
LOCUS       DAOPUR010000206         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_41476_length_2599_cov_3.553066, whole genome shotgun sequence.
ACCESSION   DAOPUR010000206 DAOPUR010000000
VERSION     DAOPUR010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41476_length_2599_cov_3.553066"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PKN90_04390"
     CDS             complement(<1..927)
                     /locus_tag="PKN90_04390"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNZ85550.1"
                     /translation="MKVLKFGGTSVGSAQRMQHVANLICDGSQKIVVLSAMSGTTNTL
                     VEISDYLYKKNQGSANEVVNTLEKKYLAVINELYTTTEYKQKATEAIKQIFSYLRSFS
                     KSPVFSLFDEKEILAQGEMMSTNMMHFYLCEQGIKSTLLPALEFMRTDKNNEPDAAFI
                     KEHIAKQLSQYTDTEVFVTQGFICRNAYGEVDNLQRGGSDYSASLIGAAILADEIQIW
                     TDIDGMHNNDPRFVENTKPVAKLHFEEAAELAYFGAKILHPTCILPAKLENIPVRLLN
                     TMDPKAAGTLISTENTKGKIEAVAAKDNITAIK"
     gene            complement(1128..2402)
                     /locus_tag="PKN90_04395"
     CDS             complement(1128..2402)
                     /locus_tag="PKN90_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ85551.1"
                     /translation="MKEITANEFDQEVLKGEKVVVDFYSTECPPCEALFPKYEAVAEL
                     YGHKIKFLKIYRQGNRELATQLGVSSSPTLLFFENGKQILDTISGGIKKSEITARLNQ
                     MLTENEIAEIQKKQQRSVSEYDVAILGGGPAGLTAGIYLGQAKVKTVLIDPALPGGYM
                     GITHQVSNYPGFVEAQNGYMLAHYMSEQAKQTGIDFKVAVDITSVDLVKKEIVIDELE
                     TIKAKKIIIATGTSPNTMNAPGEKEYKGKGISYCATCDAKYFVDKEVTVIGGGNSAVE
                     EALFISKFASKITMIHQFDALTANKTAIEQLQANEKITVKYEHEPRKFEKRGEKMVTT
                     IENLRTKEIAELASDGVFVFIGFKANLSFLSTNATPALDNWGYIQTDEDRKTSISDVY
                     AIGDIVSKKYRQITTAVADGTIAAITISKELN"
     gene            complement(2419..>2599)
                     /locus_tag="PKN90_04400"
     CDS             complement(2419..>2599)
                     /locus_tag="PKN90_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85552.1"
                     /translation="TTIEACKNCEVALACGGGCASVAKNKHGHVNSPDCRPIKELLSL
                     GAAYYFSNTPIDTKN"
BASE COUNT          679 a          481 c          494 g          945 t
ORIGIN      
        1 tttaatagcg gtgatattgt cttttgctgc tacagcttca atttttcctt ttgtgttttc
       61 tgttgaaata agtgttccgg ctgcttttgg atccattgtg ttcagcaatc gtactggaat
      121 attttctaat tttgctggta aaatgcaagt aggatgaagt atttttgctc caaaataggc
      181 gagttctgct gcttcttcaa aatgcaattt tgccaccggt tttgtattct ctacaaaacg
      241 agggtcattg ttgtgcatac catcaatatc tgtccatatc tgaatttcat cggctaaaat
      301 tgcggcacca ataagagagg cagaatagtc gcttccacca cgttgtaggt tgtctacttc
      361 cccataggcg ttgcgacaga taaatccttg agttacaaaa acctctgtat cagtatattg
      421 gcttaattgt ttggctatgt gttctttgat aaatgctgca tcaggctcat tgtttttgtc
      481 cgtacgcata aattctagag ctgggagtag agttgatttt attccttgct cacacaaata
      541 gaaatgcatc atattggttg acatcatttc accttgtgct aaaatctctt tttcgtcaaa
      601 taaactaaaa acgggcgatt tgctaaaact acgtaagtat gaaaaaattt gcttaatagc
      661 ttctgttgct ttttgtttat attctgttgt tgtgtatagt tcattaatta ccgctaggta
      721 ttttttttcg agtgtattta ccacctcatt agcactacct tgattttttt tgtataaata
      781 atctgaaatt tctactaagg tattcgttgt gcctgacata gctgataaaa caacaatttt
      841 ttgacttccg tcgcagatta agtttgctac atgttgcatg cgctgtgcgg atcctacaga
      901 tgttccacca aattttaata ctttcattct gattaagcgt taatgtttgt ttagcttgaa
      961 attttggagt acaaaggtaa tcattttact gaattaccca cttatttttg aatgtaaaaa
     1021 aatagaaaat tgaatagata cttacaagat gaggacttag aaaccaaaat atcctatttt
     1081 tgacagcaaa aatgctacaa aaataggata ttgttagagg gatcaggtta gttgagctcc
     1141 ttggatattg ttatagcggc aatggttcca tctgccacag cagtggtaat ttggcggtac
     1201 tttttgctta cgatgtctcc gatagcatac acatcagaga ttgaagtttt gcggtcttca
     1261 tctgtttgga tgtatcccca attatctaaa gctggggtag cgttggtgct aagaaaagaa
     1321 agatttgctt taaatccaat aaatacaaaa acgccatcag atgctaattc agcgatttct
     1381 ttggtgcgta agttctcgat ggttgttacc attttttcgc cacgtttttc aaatttacgg
     1441 ggttcgtgtt cgtacttaac tgtaattttt tcattagctt gtagttgttc gatagctgtt
     1501 ttgttggctg taagtgcatc aaactgatga atcatcgtaa ttttgctagc aaatttgcta
     1561 ataaacagtg cttcttctac ggctgagttt cctccgccaa ttactgttac ttctttgtca
     1621 acaaagtatt tagcgtcgca cgtggcacaa tacgaaatac cctttccttt gtattctttt
     1681 tcgcctggag cattcattgt gttggggctg gtgccagtag caatgataat tttttttgct
     1741 ttgatggttt ctaattcatc aattacgatt tcctttttca ccaaatcaac tgatgttata
     1801 tctacggcta ctttaaaatc gatgcccgtt tgttttgctt gttcgctcat atagtgtgcc
     1861 aacatatagc cattttgtgc ctctacaaag ccgggataat tagatacttg gtgtgtgatg
     1921 cccatgtatc ctcctggcaa agctgggtcg atgagcacgg tttttacttt tgcttgtcct
     1981 aaatagatgc cagcagttaa gcccgcagga ccaccaccca aaatagccac atcgtattca
     2041 cttacgcttc gttgttgttt tttttgtatc tcagcaattt cattctcagt taacatttgg
     2101 tttaaacgag ctgtaatttc cgatttcttt attccaccgc tgatagtgtc aagaatttgc
     2161 ttgccgtttt caaaaaataa aagtgtggga gacgaggaaa cgcctagttg agtggcgagc
     2221 tctctgtttc cttgtcggta aatttttaag aatttaattt tgtgtccgta caattccgct
     2281 acagcctcgt attttggaaa taaagcttcg caaggaggac attctgttga ataaaagtca
     2341 actacgactt tttctccttt taagacctct tggtcaaatt catttgcagt aatttctttc
     2401 atattctatt gtgttttatt aatttttggt atcaatgggg gtatttgaga aataatatgc
     2461 tgcacctaac gacagtagtt cttttatggg tctacagtcg gggctattta catgcccatg
     2521 tttgtttttt gcaacagaag cacagcctcc gccacaagcc agggctacct cacaattttt
     2581 acaagcctca atggtagtg
//
LOCUS       DAOPUR010000207         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_41535_length_2597_cov_2.802518, whole genome shotgun sequence.
ACCESSION   DAOPUR010000207 DAOPUR010000000
VERSION     DAOPUR010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41535_length_2597_cov_2.802518"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKN90_04405"
     CDS             complement(<1..645)
                     /locus_tag="PKN90_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNZ85553.1"
                     /translation="MTLIKSISGIRGTIGGKAGEGLSPLDIVKFTAAYATYILKNTTK
                     KTNKVVVGRDARISGEMVNNIVNGTLMGMGIDVVNIGLATTPTTELAVTMENAIGGII
                     LTASHNPIQWNAHKLLNEKGEFLDDKAGKAILEIANDESFQFADVLELGKLTENNTFN
                     QKHIDSVLSLKLVDVDAIRNAKFRVAIDCVNSVGGIIIPQLLSALGVTEIVPLYC"
     gene            complement(663..1202)
                     /locus_tag="PKN90_04410"
     CDS             complement(663..1202)
                     /locus_tag="PKN90_04410"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HNZ85554.1"
                     /translation="MELYNTLLFFSISIVLVFTQCTSEETYAQKLKKERLKFAEYVEK
                     GNIQLSSDSIYCFSLPTPWPENLYYRTPRGAYINLIDKGTSKEATEGSTVIVRYVATN
                     IAGDTIANNTSTSVSRDGYEFIYKEGSSVPCIGWNDAMLYLNNNGKAKVIVESKIGFS
                     QHQTSVITVISDVTVSITN"
     gene            complement(1264..1776)
                     /locus_tag="PKN90_04415"
     CDS             complement(1264..1776)
                     /locus_tag="PKN90_04415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNZ85555.1"
                     /translation="MACILLLASCTEPTPQNPTNKLVSTDSVQQNLLLLNQAFVALEQ
                     QEINEYIAQHRLNVHLSENGYWYRIDKRGTGNTLKANQLVTIEYYLELLDGTLCYSSE
                     KNGIKQFRVGKFEVEKGLDDFMLLLSLGAEATCIIPSYLAHGVVGDRNCIPPRTPIVY
                     RIKVISIKDN"
     gene            complement(1825..>2597)
                     /locus_tag="PKN90_04420"
     CDS             complement(1825..>2597)
                     /locus_tag="PKN90_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNZ85556.1"
                     /translation="IANLYEKLLNNNISVVAANKIAASSDYDHYMHLKSLARKKGIKY
                     LFETNVGAGLPIINTINNLINSGDKILKIEAVLSGTLNFIFNTISEKISFSQSIQMAK
                     EARYAEPDPRIDLSGKDVIRKLVILARESGYKIDQDDVAKNLFVPDSYFQGSVDDFWN
                     TIGELDAEFEEKRKKLAAQDKKYRFVAKMDNGKCSVGLQEVDARHPFYDLEGSNNIIM
                     IHTERYFEYPMIIKGYGAGASVTAAGVFGDIISIANIR"
BASE COUNT          794 a          469 c          404 g          930 t
ORIGIN      
        1 gcaatataaa ggtacaattt ctgttacccc taatgctgat aaaagttgag gaattataat
       61 acctccaact gaattgacac agtcaatggc aacacggaat tttgcattgc gtatagcatc
      121 tacatccact agtttgagtg aaagaacgct gtcgatatgt ttttgattga atgtattatt
      181 ttcagttaat ttacctaact ccaatacatc tgcgaattga aagctttcat cgttagcaat
      241 ttctaaaatt gctttacctg ctttatcgtc taaaaattct cccttttcgt ttagcagttt
      301 gtgtgcattc cattggattg gattatggct agcagtaagt ataataccgc caatagcatt
      361 ttccatggtt actgctaatt ctgtagtagg agtagtagct aatcctatat ttactacatc
      421 gatacccata cccattaatg tgccatttac aatattatta accatttcac ccgaaatacg
      481 agcatctcta cctactacca ctttattggt tttttttgta gtgtttttaa ggatgtatgt
      541 ggcataagca gctgtaaatt ttactatatc caatggactc aacccttcac cagcttttcc
      601 tcctatggta cctctaatac ctgaaataga ttttattaat gtcataattc tgtgtttgta
      661 tattaatttg taatagatac agtcacgtca gaaattactg taataacaga agtttggtgt
      721 tgcgaaaatc caattttaga ttcaacaata actttcgctt taccattgtt attcagatac
      781 aacatagcgt cattccatcc tatacaaggt acactacttc cttctttata aataaattca
      841 tatccatctc tacttacaga tgtagaggtg ttatttgcaa ttgtgtctcc agctatattg
      901 gttgctacgt atcttacaat tacggttgaa ccttctgtcg cttcttttga agtcccctta
      961 tctatgaggt taatatatgc ccctcgaggt gttctatagt ataaattttc gggccatggg
     1021 gtgggtaaag aaaaacaata aatagaatca gatgataact gaatatttcc tttttctaca
     1081 tattcggcaa attttaagcg ttctttttta agtttttgtg catatgtttc ttccgaagta
     1141 cattgtgtaa aaacaagtac tatgctaata gagaaaaata aaagtgtgtt gtataactcc
     1201 ataaaatgat atttaaataa acgtacgcaa aagtacacat aaaaattgtg ttatgattga
     1261 gtattaatta tctttaatac ttattacttt gattctatat acaattggtg tacgaggagg
     1321 tatacaattt ctgtctccaa ccacaccatg agctaagtac gaaggaatga tacatgtagc
     1381 ttcggctccc aatgagagta ataacatgaa atcatctaat cctttttcta cttcaaattt
     1441 acccacccta aattgtttta taccgttctt ttctgaagaa taacaaaggg ttccatcaag
     1501 tagttcaagg taatattcaa tagttactaa ttgattggct ttaagcgtat ttcctgtacc
     1561 tcgtttatct atacgatacc agtagccatt ttctgacaaa tgtacgttta acctatgttg
     1621 agcaatgtat tcgtttattt cttgttgttc aagagccaca aaggcttgat ttaaaagtaa
     1681 taagttttgt tgcacactat ctgttgatac tagcttattg gttgggtttt gtggagtagg
     1741 ctcagtacaa ctagcaagaa gcagtataca tgccattatc caccagtttt ttttactatt
     1801 aaccaacatt agagatgttt tttcttatcg aatattagca atactaataa tatctccgaa
     1861 tacgccagct gctgtaacac tagcaccagc accatatcct ttaatgatca ttggatattc
     1921 aaaataacgt tcagtatgta tcataatgat attgttgctc ccttctaaat cgtaaaatgg
     1981 gtgtcgtgca tccacttctt gcaaacctac tgagcatttt ccattatcca tttttgctac
     2041 aaagcggtat tttttgtctt gagcagctaa ttttttacgc ttttcttcaa attcagcatc
     2101 taattcgcca atagtattcc aaaaatcatc aactgaaccc tgaaaatacg aatcaggaac
     2161 aaacaaattt tttgccacat catcttgatc tattttatag cctgactcgc gagctaagat
     2221 taccaattta cgaattacat cttttccgct taaatcaatg cggggatcag gttcagcata
     2281 gcgagcctct tttgccattt gtatagattg agagaaagat atcttttcgc ttatagtatt
     2341 gaaaataaaa tttaatgtgc cagaaagtac agcctcaatt tttaatattt tatccccact
     2401 attaattaaa ttgttgatag tgttaataat gggtaatcca gcaccaacat tggtttcaaa
     2461 taggtattta attccttttt tgcgagccaa agattttaag tgcatataat gatcatagtc
     2521 ggatgatgcc gctattttat tggcagccac aacagagatg ttattattaa gaagtttttc
     2581 gtataggttt gcaattt
//
LOCUS       DAOPUR010000208         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_41911_length_2583_cov_2.689873, whole genome shotgun sequence.
ACCESSION   DAOPUR010000208 DAOPUR010000000
VERSION     DAOPUR010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41911_length_2583_cov_2.689873"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="PKN90_04425"
     CDS             complement(<1..1818)
                     /locus_tag="PKN90_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNZ85557.1"
                     /translation="MENEMNYSDNVADILRYSHEEAERLGNNYIGPEHLLLAIIRAGE
                     GKAFDVLLSLNLDLLRIKKTIESSIRTSENVMIGSNIPLLKSTEKILKFIVLEARQFG
                     NYMADSEHLLLAILKDKTNLVTEIFSVENISYDTVRALVEKKDIGSIDKKNGVNSNDE
                     DGEDEDSEFSSNSVEKNGKTSTFSTSTKYTKNTSKTPALDNFGTDITKAAEEGKLDPI
                     VGREKEIERLSQILSRRKKNNPILIGDPGVGKSAIVEGLALRIIQRKVSRILFGKRVV
                     SLDMASIVAGTKYRGQFEERLKAILNELETNPDVILFIDEIHTLIGAGATAGSLDAAN
                     MMKPALARGLIQCIGATTLDEYRQSIEKDGALERRFQKVIVKPSTKEETLAILQNIKE
                     RYEDHHHVTYTPEALEACVYLTDRYISDRSFPDKAIDAMDEAGSRAHLHNVSIPSNIE
                     ILESEIDEANKSKLQAVKDEDFRLANEYKDRIKELNILLKNAKQEWEQEIKNQHVTVD
                     ANSISEVVSMISGVPVQQIAEGEFDKLRRMSAILKEKVIGQDTAIEKISKSILRNRVG
                     LKDPNKPIGSFIFLGPTGVGKTYLAKILAEFLFNSKDAII"
     assembly_gap    2199..2208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2209..>2583
                     /locus_tag="PKN90_04430"
     CDS             <2209..>2583
                     /locus_tag="PKN90_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNZ85558.1"
                     /translation="PVHRRVLFGMNELGSNSNKPYKKSARIVGEVMGKYHPHGDSSIY
                     GTLVRMAQEWSMRYQLVDGQGNFGSIDADEPAAMRYTEARMHRLAEEMLMDIDKDTVD
                     FKLNFDDTLKEPTVLPTRIPNL"
BASE COUNT          721 a          460 c          453 g          939 t
ORIGIN      
        1 aataatagca tctttagagt tgaacaaaaa ttcagctaaa atttttgcta aataagtttt
       61 tccaacccct gttggtccta aaaatataaa tgagccaata ggtttatttg ggtcttttaa
      121 accaactcta ttgcgtaaaa tagatttgga aattttctca atcgcagtat cttgtccaat
      181 tactttttct tttagaatag ccgacatacg tcttagctta tcaaattcac cttcggcaat
      241 ttgttgaaca ggaaccccag atatcatcga tacaacctcc gaaatgctgt tggcatctac
      301 agtaacatgt tggttcttaa tctcttgttc ccattcttgt ttggcatttt ttagcaaaat
      361 gttcaattcc ttgatgcggt ctttgtattc attggctaat cgaaaatctt catcttttac
      421 agcttgaagt tttgatttat tggcttcatc aatctcactc tccaaaatct caatattgct
      481 tggaatggat acattgtgta agtgtgccct tgatcctgct tcgtccatgg catcaatagc
      541 tttgtctgga aaacttcgat cgcttatgta cctatcagtt aaatagacac atgcttctaa
      601 tgcttctggt gtataggtta catgatggtg atcttcgtat ctttctttga tattttgtaa
      661 aatagccaag gtttcttctt ttgtgcttgg ttttacaatt accttttgga atcgtctttc
      721 taatgcaccg tctttttcta tactttggcg gtactcgtca agtgtagtgg caccaatgca
      781 ttgtatcaag cctcgagcca aagctggttt catcatgttt gcagcatcta acgagcctgc
      841 tgttgcacca gcaccaatta aggtatgtat ttcgtcgatg aataaaataa catcagggtt
      901 tgtttccaat tcatttaaga ttgcttttaa tctctcttca aactgtcctc tatactttgt
      961 tcctgctaca atggatgcca tatctaatga tactacacgt ttgccgaata atatacgaga
     1021 tacttttcgt tgtataatac gtagagccaa tccttcaaca atagccgatt ttccaacacc
     1081 tgggtcgcca atgagtatag gattattctt ttttcttcta cttaaaattt gagataggcg
     1141 ttcaatttct ttttctctac caacgattgg gtctaacttt ccttcttcgg ctgctttggt
     1201 aatgtctgta ccaaaattgt cgagtgcagg tgttttagaa gtgttttttg tgtatttagt
     1261 tgatgtagag aaggtgcttg ttttaccatt tttttctaca gaatttgagc taaattcact
     1321 atcttcgtct tcgccatctt cgtcattcga gtttactccg ttttttttat caatagatcc
     1381 aatgtctttt ttttctacta aagctcttac tgtatcatac gaaatgtttt ctacactaaa
     1441 aatttctgta accagatttg ttttatcttt taatattgct agtaaaaggt gctcagaatc
     1501 agccatatag ttgccaaatt gacgagcctc aagtacaatg aatttgagta ttttttcagt
     1561 tgattttagc aggggtatat tggatccaat catcacgttt tctgatgttc ggatactcga
     1621 ttcaattgtt ttttttatac gcaataaatc taagttaagt gacaaaagta cgtcaaaagc
     1681 tttaccctct cctgcacgaa taattgccaa aagtaagtgt tcgggtccaa tgtaattatt
     1741 gcccaaacgt tctgcttctt cgtggctata tcgtaaaata tcggcaacat tgtccgaata
     1801 gttcatttca ttttccatat tcttacgtgt ttgataaaca ttattgttag tgtttgctaa
     1861 ctattttgca catattgtat tgcaaaggta tagtttttct ttaaaaattg tgtattgttt
     1921 acacattctt aacaacagat tacaaatact atgccaacaa tatatgtgtg tgttaaaaag
     1981 atttttgacg aaaggttttt gtatttgaat aaaaagtagt accttagtgc gaattttcag
     2041 aaaaggtatt tgttttactt aactatttta taaatgattg atcaagaaag gattataaaa
     2101 atcaacattg aggaggagat gaagtcagca tacattgatt actcaatgtc tgttattgta
     2161 tcgcgtgcac ttcccgatgt aagagatgga ttgaagccnn nnnnnnnnag cctgtacatc
     2221 gtagagtatt gttcggaatg aatgagttgg ggagtaactc caataaaccg tataaaaaat
     2281 ctgctagaat agtaggggaa gtgatgggta agtatcaccc tcatggcgat tcttctattt
     2341 atggtacact agtgcgaatg gcacaagaat ggtcaatgcg ttatcaactg gttgatggtc
     2401 agggaaactt tggctctatt gatgccgacg aacctgctgc tatgcgttat acggaagccc
     2461 gtatgcatcg cttggcagaa gaaatgttga tggatattga taaagatacc gttgatttca
     2521 aacttaattt tgacgataca ttaaaagaac caactgttct gccaactcgt atacccaatt
     2581 tat
//
LOCUS       DAOPUR010000209         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_41958_length_2581_cov_3.620348, whole genome shotgun sequence.
ACCESSION   DAOPUR010000209 DAOPUR010000000
VERSION     DAOPUR010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41958_length_2581_cov_3.620348"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKN90_04435"
     CDS             <1..510
                     /locus_tag="PKN90_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNZ85559.1"
                     /translation="IVYVHSPAGIFPKISIPFDRIIERVPSSSYNTINHIHLIAEAAI
                     LDDNSTYSEPSTLLLIREQDIEKFFAQSLFPATSIGIYAFLGTYNSTSKSYTFTSMAD
                     YFRMILKNEGDDANTLNDFVLVPVKTITNTRGTITSLRHLYNPRGIRLRSGKNITSPM
                     RIEITYSQF"
     gene            complement(562..813)
                     /locus_tag="PKN90_04440"
     CDS             complement(562..813)
                     /locus_tag="PKN90_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNZ85560.1"
                     /translation="MVKTMTFNELRKIKDSLPDGTIRKIAQQLNITEETVRNYFGGHD
                     FDKGKAVVGVHIEPGPDGGVVTLDDTTILDLAMQEMARV"
     gene            complement(913..1641)
                     /locus_tag="PKN90_04445"
     CDS             complement(913..1641)
                     /locus_tag="PKN90_04445"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNZ85561.1"
                     /translation="MNYLEIVTNIFSSIVSWDTNMLLKLNSWNNLFFDGFMWITTSIL
                     VWLPLYISVIYIIIKNKKKESIIILFSLMLAIVLADQLASSVLKPLVERLRPTHNEDL
                     MPYIHTVNGYKGGIYGFVSSHAANAFAFVVFTAFLFRQFSYINLMLLWALLLSYSRIY
                     LGVHFPLDVIVGALLGGGIGYAAFVFYQLLKRIMPQYVYKKHKTNSRHYSPFSVKDLS
                     PFFFCFVITLFFSILFAYNLSFFE"
     gene            complement(1647..2411)
                     /locus_tag="PKN90_04450"
     CDS             complement(1647..2411)
                     /locus_tag="PKN90_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ85562.1"
                     /translation="MKYGLLIQLFLFFHISLSLNATTAQVYDIKKANDLYVATSYAQA
                     IEVYEEISKQGYESATLYYNLGNAYYRINKIAPAILNYERALRLDPSNLDIQHNLKLA
                     KAKTVDKIVTQEKNIIEIWYNSIVFATHTNVWAYMSIIFFIVALCLVLMYMFLRVSWI
                     RKITFFSSIIFFTFAICFFIFSYQQKKMVENQKEAIIFVPSSYVKSSPDQLGSDLFLL
                     HEGTKIIIKHQLGSWVQITTEDGNKGWLPVSTIQKI"
BASE COUNT          953 a          422 c          354 g          852 t
ORIGIN      
        1 attgtatatg tacattctcc tgcgggtatt ttcccaaaaa tctctatccc ttttgataga
       61 ataattgaac gagtgccaag ttctagttat aataccatta atcatatcca tttaattgca
      121 gaagcagcta tattagacga taatagtact tatagtgagc catctaccct tctgctgata
      181 agagaacaag atatagaaaa attctttgct caaagtttat ttcctgccac atccattggc
      241 atctatgcat tcttaggcac ctataactcc acatcaaaga gttatacatt tacttcgatg
      301 gctgattact ttagaatgat actgaaaaat gagggggatg atgcaaatac gctaaacgac
      361 ttcgtattag tgccagtaaa aaccattaca aatacaagag gtactattac aagcttacga
      421 catttgtata atcctagagg gataaggcta cgcagtggca agaatataac atctccaatg
      481 cgtatagaaa ttacatatag ccaattttga ttacaaaaaa aaagaggaaa attcctcttt
      541 tttttgtaat gtcttaaaaa attaaactcg agccatttct tgcatagcta aatctaaaat
      601 ggtagtatca tctaatgtaa ctacaccacc atcaggacca ggttcaatgt gtacaccaac
      661 aacagcttta cctttatcga aatcatgccc cccaaaataa tttctaactg tttcttcagt
      721 aatgtttaac tgttgtgcaa tttttctgat tgttccatca ggtaaactgt ctttgatttt
      781 tctcaactcg ttgaatgtca tagtttttac cataatttca cagtattaaa ttaataaata
      841 aataattatg tttttaaagt tgcaataaat atacgtcaat attttatata aaaaaaatat
      901 ttgacacaaa atttactcga aaaaacttaa attatatgca aatagtatac taaagaagag
      961 ggttataaca aaacagaaaa aaaatgggga taaatcttta acagaaaatg gagaataatg
     1021 tctactattt gttttatgtt ttttatagac atactgaggc attattcttt ttaacaattg
     1081 atagaataca aaagctgcat acccaatgcc tcctcctaat agtgcaccca caatcacatc
     1141 taaaggaaaa tgaactccta aataaatgcg agaataagac agtaataatg cccaaagtaa
     1201 cattaaattt atatacgaaa attgtctaaa tagaaacgct gtaaaaacta caaaagcaaa
     1261 cgcatttgct gcgtgtgaag atacaaatcc ataaatacct cctttatatc catttactgt
     1321 gtgaatatat ggcattaaat cctcattatg agtaggtctc aatctttcaa caagtggttt
     1381 taaaacagat gatgctaact gatcggctaa tacaatagct aacattaaag aaaacaatat
     1441 aataattgat tctttctttt tattttttat aattatatat ataaccgaaa tataaagagg
     1501 taaccagact aaaatagagg ttgtaatcca cataaaaccg tcaaaaaaaa gattgttcca
     1561 actatttagt ttcaacaaca tatttgtatc ccaactaacg atagaagaaa aaatgtttgt
     1621 tacaatttct aaataattca tgtagtttat attttttgaa tagttgaaac aggcaaccat
     1681 cctttatttc catcttctgt tgttatttgt acccaagagc ccaattgatg tttaatgata
     1741 atttttgttc cttcatgtaa taagaataaa tcactaccta attgatcagg agaacttttt
     1801 acataagaag atggcacaaa gataatagct tctttttggt tttctaccat tttcttttgt
     1861 tgatacgaaa aaataaaaaa gcatatcgca aatgtaaaaa atataatgga agaaaaaaac
     1921 gttatttttc taatccaact tacacgtaaa aacatataca ttaatacaag acataatgct
     1981 acaataaaaa aaatgatact catataagcc cacacatttg tatgagtagc aaatacgatt
     2041 gaattgtacc aaatttcaat tatgttcttt tcttgtgtaa caattttatc tactgtttta
     2101 gcttttgcaa gtttcaagtt atgttgtata tctagattgg atggatctaa acgcaaagct
     2161 ctttcataat taagaatggc tggggcaatt ttatttatac gataataagc atttcctaag
     2221 ttataataca atgtggcaga ttcataacct tgcttactta tttcttcgta tacctcaatg
     2281 gcttgtgcat acgacgtagc cacatataaa tcattggctt ttttaatatc atatacctga
     2341 gcagtagtag catttaggga caatgatata tgaaaaaaaa gaaacaattg gattaacaaa
     2401 ccgtatttca tatcattatt ctttatgttt tagttcactt tcaagacttg taataatatt
     2461 acatgctctt tggtacattg tttccatagc aagctcagag ctcgaagaag gagaaaaacg
     2521 tgcaaattca caatcattta agaccgcaat agtttcgtca atacatgtac tagcaactcc
     2581 t
//
LOCUS       DAOPUR010000210         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_41989_length_2580_cov_2.712871, whole genome shotgun sequence.
ACCESSION   DAOPUR010000210 DAOPUR010000000
VERSION     DAOPUR010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41989_length_2580_cov_2.712871"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKN90_04455"
     CDS             complement(<1..245)
                     /locus_tag="PKN90_04455"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNZ85563.1"
                     /translation="MSGVVLTSTYLGNIQFYSKLIHFSEIWIEQHCHYTKQTYRNRCK
                     IATANGVMALSIPTIKPATEKCKTKDIRIDNSQHWQQ"
     gene            complement(249..1676)
                     /gene="lepB"
                     /locus_tag="PKN90_04460"
     CDS             complement(249..1676)
                     /gene="lepB"
                     /locus_tag="PKN90_04460"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNZ85564.1"
                     /translation="MSDIHNEKKTGRFDVSLIQWIKAFVVCLLYVLFIIWVGNFWWLL
                     LLPIFFDIYITKFIPWSFWKKSKNKYVLKIAEWTDAIVFALIAVYFINLFLFQNYKIP
                     TSSLEKSLLVGDFLLVSKVSFGPRVPNTPLSFPLAQHTLPILNTKSYIEYPQWEYKRL
                     KGLDTIKHLDIVVFNFPAGDTVTTKVSNPDYYTLCHDYGREYVHNNKNLFGDVIYRPV
                     DRRENYVKRCVGLPGDTLQIIEHQVYINGVAQEEVEGTQLSYWVTTDGRKISENQFKR
                     IGVSKDDQQFLINNLPNGDNLLDYCGYERNSEGKHNPVYRLPLTKEALNKVKNLPNVI
                     KIEIDNGLISGQVFPFGYKKWTRDNYGPIYIPRKGATIALNLDNLPVYERVIRNYEGN
                     TLEIKDSTIYINAVATTNYTFKMNYYWLMGDNRHNSADTRYWGFVPEDHVVGKPLFVW
                     LSLDKDASLLESIRWERLFKFVHKE"
     gene            complement(1692..2408)
                     /gene="dapB"
                     /locus_tag="PKN90_04465"
     CDS             complement(1692..2408)
                     /gene="dapB"
                     /locus_tag="PKN90_04465"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNZ85565.1"
                     /translation="MKLALIGYGKMGKEIEQIAISRGHEIVSRIDVDNQEDFKSDAFK
                     SADVAIEFTVPHVAYANYLKAFEAGVSIVSGTTGWLDKINEIKAICHDKQLAFFWASN
                     FSLGVNIFAAVNSYLAKIMNQYSEYNVSMTEVHHTQKLDAPSGTAITLAEGIIKELAR
                     INKWELETASTKDSLAIHAIREGNVPGIHTIRYESEVDSIEITHDAKSRKGFALGAVL
                     AAEFTYGKQGFLGMNDLLQF"
BASE COUNT          758 a          492 c          389 g          941 t
ORIGIN      
        1 atttgttgcc aatgctggct attatctatg cgaatatctt ttgttttgca tttttctgtt
       61 gcaggcttta tggttggtat tgataacgcc ataactccgt ttgcagtagc aattttacac
      121 ctatttcgat aggtttgttt tgtatagtgg caatgttgtt ctatccaaat ttcagaaaag
      181 tgtatcaatt tactgtaaaa ctgaatattc cctaaatacg tggatgtgag tactacaccg
      241 ctcatttctt attctttatg cacaaattta aataatcttt cccatctaat gctttctagc
      301 aacgatgcat ctttgtctaa tgataaccat acaaatagtg gttttcccac cacatgatct
      361 tctggcacaa atccccaata acgagtatct gctgaattgt ggcggttatc tcccattaac
      421 caataataat tcatcttaaa ggtgtaatta gtcgttgcaa cagcatttat ataaattgta
      481 ctatccttaa tttctaaagt gttgccttcg taattgcgaa ttacacgttc atatacgggt
      541 aaattatcca aatttaatgc aatggtagcc cctttgcgag gaatataaat aggtccataa
      601 ttatcgcggg tccatttttt ataaccaaat gggaatactt gacctgaaat taatccatta
      661 tcaatttcta ttttgattac attcggtaaa ttttttacct tgtttaaagc ctctttggtt
      721 aatggtaatc tgtatactgg attgtgtttg ccttcactat ttctttcata gccacaataa
      781 tccagcaagt tatcaccatt tggcaggtta ttgattaaga attgttggtc atctttgctt
      841 acccctatac gtttaaattg gttttccgaa atttttctac cgtcagtcgt aacccaataa
      901 cttaattggg tgccttctac ttcttcttgg gcaactccat ttatatatac ttggtgttca
      961 attatttgta atgtgtcgcc aggtaatcct acgcatcttt ttacatagtt ttctcgccta
     1021 tctactgggc gatagatgac gtctccaaac aaatttttat tattgtgtac atattctctt
     1081 ccgtaatcat gacataatgt atagtaatct ggatttgata cttttgttgt tactgtgtcg
     1141 cccgcaggaa aattaaatac tacaatatct aaatgtttaa ttgtatctag ccctttcaat
     1201 cgtttatatt cccactgagg gtattcaata tacgatttgg tattgagtat aggtaatgtg
     1261 tgttgtgcca atggaaaaga aagtggcgtg tttggaactc ggggtccaaa gcttactttg
     1321 cttactaaca aaaaatcgcc taccaacaat gatttttcca atgatgaggt aggaatttta
     1381 taattttgga ataaaaataa gttgataaaa tacaccgcaa tcaatgcaaa tacgatagca
     1441 tctgtccatt cagcaatttt aaggacatat ttatttttcg attttttcca aaaactccaa
     1501 ggaataaact tcgtgatata gatatcaaaa aatataggaa gtagtaataa ccaccaaaaa
     1561 tttcctaccc agataataaa tagtacgtat agcaagcata caacaaacgc ttttatccat
     1621 tgtattagtg aaacatcaaa acgccctgtt tttttttcgt tatgtatatc gctcatcttt
     1681 tttatctttg tttaaaattg taataaatca ttcattccta agaatccttg tttgccatac
     1741 gtaaactcag cagccaaaac cgcacccaat gcaaaccctt tgcggctttt agcatcgtgc
     1801 gttatttcaa tgctatctac ctccgattcg tacctaattg tgtgtatgcc aggtacattc
     1861 ccctctctaa tagcatgtat tgccagcgaa tcttttgtag atgccgtttc taattcccat
     1921 ttatttatac gtgctagctc cttaatgatt ccttcagcca atgtaatggc agttccactc
     1981 ggtgcatcta atttttgtgt atgatgaact tctgtcatag aaacattgta ttcagaatat
     2041 tgattcataa tttttgccaa atagctattt actgcagcaa aaatattaac tcctaaactg
     2101 aaattggatg cccaaaaaaa tgccagttgc ttgtcgtggc aaattgcttt aatttcatta
     2161 attttatcaa gccatccagt agtgcccgaa actatcgaaa cgcctgcttc aaatgccttt
     2221 aagtaattgg cataagccac atgaggcacc gtaaattcta tcgctacatc ggcactttta
     2281 aacgcgtctg acttaaaatc ttcttggttg tcaacatcta ttctgcttac tatttcgtgt
     2341 ccacgagaaa tggcaatttg ttcaatttct tttcccattt taccatatcc aatcaaggct
     2401 aatttcataa ttataagttt aggtttttat tggttgtttt atgcaaaaaa actataactt
     2461 gcaaatatac aaataatgta taaaaattgg agtccattcc taaaataatt acatattcag
     2521 ttgtttttgc tccttttttg tcaatccttt aatcactaat tcgtacgatt cgtctatcca
//
LOCUS       DAOPUR010000211         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_42096_length_2575_cov_3.460714, whole genome shotgun sequence.
ACCESSION   DAOPUR010000211 DAOPUR010000000
VERSION     DAOPUR010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42096_length_2575_cov_3.460714"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PKN90_04470"
     CDS             complement(<1..1107)
                     /locus_tag="PKN90_04470"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNZ85566.1"
                     /translation="MATLEKIRQKGVLLTVIIGGALLAFIIGGIDFNTMRQGARETVA
                     VINGEEIKIADYQKRIDEMTAFYKIELGQSNLSEEYVEQINNAVWNTWSNEQLIGKQA
                     QSVGLVVTDKELADAVYGDNIHPLFYSIRMFYNNQGQFDKTLLLQFLNSISQDTSGEA
                     AKYWGFIERMLKNNILEEKYYTLVSNSFNANDIDTKYAFDARTITTGLEFVAQPYFSL
                     SDSLFQVTDKEIKDRYKRDQIRYTQQDETRDIAIVTFDIKPSEQDFQEVKLWIEKLKN
                     EFSTSTDFVSIANQNSDVPTSDIAVSRNNIDPDLKEFAFTASVGDVFGPIYVNGTYKM
                     ARLSEKGILSSDSAKVRHILIQEPTVEATQKLVDS"
     gene            complement(1219..2484)
                     /locus_tag="PKN90_04475"
     CDS             complement(1219..2484)
                     /locus_tag="PKN90_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ85567.1"
                     /translation="MTTTVFLIFISLLFSAFFSGMEIAFYVSNKLRFEINKQHSLLSS
                     SILSIFYANPSKYISIILVGNNIALVVYGIQMAKFLEPYLLLFLHNDLLIATLQTIFS
                     TIVILLTGEFLPKVLFRLNADLWLKVFAIPLFLCYLFLYPVATFVYQISIFLFKILRV
                     DVSNSAGGMTLGRADLDNFLQQNYENTTDKKHIENEVKLFQNALDFSKIKLRDCIIPR
                     TEILAVDFDTDIEELRVKFVESGYSRILVFKEHIDNMVGYIHSIELFKKPRKWQDQIR
                     VLPIVPETMAANKLMNYLLQKKKSMALIVDEFGGTSGMVTLEDIVEEIFGEIEDEHDT
                     QDYVAKKISENEYVFSGRLEIDYVQEQFDIQLPVSDEYLTIAGLILHYYQGFPKINEE
                     IIIGNYMCKVIKASANKIELIKLKVLATL"
BASE COUNT          878 a          459 c          343 g          895 t
ORIGIN      
        1 gctatcaacg agtttttggg tagcttcaac ggttggctct tgtattaaaa tatgacgaac
       61 cttagcagag tctgacgata gaataccttt ttccgacaaa cgagccattt tgtaggtacc
      121 attcacatat attggaccaa atacatcacc caccgaagct gtaaatgcaa actcttttaa
      181 atcaggatca atgttatttc tgcttacagc aatatcactc gttggaacat ctgaattctg
      241 attcgctatt gaaacaaagt cagtagaagt agaaaactca tttttcaatt tttctatcca
      301 caattttact tcttgaaaat cttgttcaga aggtttgata tcaaatgtta ctatagctat
      361 atctctcgtt tcatcttgtt gggtatacct aatttgatct cgtttataac gatctttaat
      421 ttctttatct gttacttgga acaatgagtc agacaacgaa aaatatggtt gagcaacaaa
      481 ctccaatcca gtagttatag tacgtgcatc aaaagcatat tttgtatcta tatcattcgc
      541 attaaaggaa tttgacacta aagtataata tttctcttca agtatattat ttttaagcat
      601 tctttcaata aatccccaat atttagcagc ttcacctgac gtatcttgac ttattgaatt
      661 taaaaactgt aataacaagg tcttatcaaa ttgtccttgg ttgttataaa acattctaat
      721 agaataaaaa agtggatgta tgttatctcc atacactgca tctgctaatt ccttatcagt
      781 aacaaccaaa cctacacttt gagcttgttt gcctatcaat tgctcattac tccaagtatt
      841 ccacacagcg ttgtttattt gctctacata ctcctcagaa aggttagact gacccaattc
      901 tattttgtaa aatgcagtca tttcatcaat tcgtttttgg taatcagcaa tttttatttc
      961 ttccccattg ataacagcaa ccgtttctct agctccttgt ctcatggtat taaaatcaat
     1021 acctccaatg atgaacgcca acaaggctcc tccaataata acagttaata aaacaccttt
     1081 ctgtctgatt ttttctaatg ttgccatgat gtatacttat aaatctttat aataattaga
     1141 taatttaggg tgcgaatata caaaaaatat tacacttagt aagtgtaaat atgattttct
     1201 tcggtttttt atatcaagtt atagtgttgc caacactttc agtttaatta attctatttt
     1261 attagctgaa gctttaataa ctttacacat atagttacca ataattattt cttcatttat
     1321 cttaggaaac ccttggtaat aatggagtat aagaccagcg attgttaaat actcatctga
     1381 aacagggagt tgaatatcaa actgttcttg tacataatct atttctaatc ttccagaaaa
     1441 cacatattca ttttcactaa ttttttttgc tacgtaatct tgagtatcgt gttcatcttc
     1501 aatttcacca aatatttctt ctacaatatc ctctaaagtt accatcccag aagttccgcc
     1561 aaattcgtcc acaatcaatg ccatactttt ctttttctgc aacaaataat tcatcaactt
     1621 attagccgcc attgtttcgg gcacaattgg caacacgcgt atctgatctt gccattttcg
     1681 tggtttttta aataattcaa ttgaatgaat ataaccaacc atattatcta tatgttcttt
     1741 aaacaccaat atacgcgaat aaccagactc aacaaatttt acacgtaatt cttcaatatc
     1801 agtatcaaaa tcgaccgcta atatttctgt acgaggaata atgcaatctc gcaactttat
     1861 tttagaaaaa tctaaggcat tttgaaacaa tttgacttca ttttctatgt gttttttgtc
     1921 agttgtattt tcataatttt gttgtaaaaa gttatctaaa tctgctcgtc caagagtcat
     1981 cccccctgct gaattagaaa catctactct cagaattttg aataaaaaaa ttgatatctg
     2041 atatacaaac gtagctacag gatataaaaa taaataacat aaaaacaacg ggatagcaaa
     2101 aacctttaac caaaggtccg cattaagcct aaataacact tttggcaaaa attctccagt
     2161 taaaagaatc acaattgttg aaaaaattgt ttgtaaagta gctatcaaca agtcattatg
     2221 taagaataac aataaatatg gctctaaaaa ttttgccatc tgaataccat atactacaag
     2281 agcaatatta ttaccaacta aaatgattga aatatattta ctaggattgg cataaaaaat
     2341 tgaaagaata gacgaagata gtaagctgtg ttgtttattg atttcaaaac gcagtttatt
     2401 tgaaacataa aaagcaatct ccattcctga aaaaaaagca gagaaaagca gagatataaa
     2461 tatcaaaaaa acagttgttg tcattgttct atcggaaata taccttgagg ttttgttatt
     2521 gtatactgtg taagagtatg gttagattga aaacctatgc ctgtaataat acgtt
//
LOCUS       DAOPUR010000212         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_42318_length_2567_cov_3.507564, whole genome shotgun sequence.
ACCESSION   DAOPUR010000212 DAOPUR010000000
VERSION     DAOPUR010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42318_length_2567_cov_3.507564"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKN90_04480"
     CDS             complement(<1..596)
                     /locus_tag="PKN90_04480"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNZ85568.1"
                     /translation="MRKSIFLYFLLSVYWLTQWLIVPCYAEIPFRVMCYNVENAFDCE
                     NDSLTNDDDFTPQGAYHWTPKKFKLKISNIAKVITAVGQFTPPSIVGLCEIENESVLK
                     SLVYFSPLRNLNYKYVHYDSPDKRGIDVALLYRPEQFRVLSSAPIGVSFASAPFSTTR
                     DILYVKGIAMYTDTLHIFVCHFPSRLGGELESEDRRIEV"
     gene            complement(599..1996)
                     /locus_tag="PKN90_04485"
     CDS             complement(599..1996)
                     /locus_tag="PKN90_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85569.1"
                     /translation="MVWIGFSSCEDMMFSTNPNQRIHISADTVSFDTLFTGVGSTTKI
                     FNIYNPSKNRIKIDAIELVGATHYSLNINGVLTDALHDISLKANDSMRIFVQAYVDPS
                     DQFTPFIVKDSIIIRSNTHTYKVVLQAYGQDAYRVSKRQIMADTTWLAEKPYLILDTL
                     TIAQGATLTLSEGVSLYFVKHASLQVYGTLKAKGIQSKPVVFRGDRMDNMFDNLPYDK
                     VPNQWQGIRFRKGSAHNELNYVVVKNTNQGIVLDSTSLDVLALAISNSIINNSATNLI
                     TASHTVMHISNSVVYNAGQSCLVLQGGKYNVVHSTIANYFSFPWVGRKATSVLLSNYI
                     NLGSQAMPVPLLEANFINSIVYGSWSNEVSVSSSYLNQKIEEPLVYVFKNSVIKISPA
                     TINDTNFLEIIANQDPKFNATKPYNYDFTLQSSSPALGNADNLVAAEYPIDMLGRQRL
                     FNDKADIGAYVIIAE"
     gene            2153..2440
                     /locus_tag="PKN90_04490"
     CDS             2153..2440
                     /locus_tag="PKN90_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85570.1"
                     /translation="MANKRNLKKDIQSIFSELLVECYTLSYIFPQIDIAKVDAIATSI
                     IEKSDNFVKRAGKPDGKDNPAIVKAYYKKLYKDVANTVSVFIEDINTLKSK"
BASE COUNT          913 a          466 c          424 g          764 t
ORIGIN      
        1 acctctattc gcctgtcttc cgactctagt tcaccaccta atcgggaagg gaaatggcac
       61 acaaaaatat gtaatgtatc ggtatacata gctatacctt ttacatacaa tatatctctg
      121 gtagtagaaa aaggtgcact agcaaacgaa accccaatag gtgcagaaga tagcactcta
      181 aactgctctg gacgatacaa aagagcaaca tctataccac gcttatcagg cgaatcgtaa
      241 tgaacatatt tataattcaa atttcgtaaa ggagaaaaat acaccaacga ttttaatacc
      301 gattcgtttt ctatctcgca caaacccaca atcgatggtg gagtaaattg tccaacagcc
      361 gttataactt tagcaatatt ggaaattttt agtttaaact ttttgggagt ccaatgatag
      421 gctccctgtg gtgtaaaatc atcatcattg gtaagagaat cattctcgca gtcgaatgca
      481 ttttcgacat tataacacat tacacgaaac ggtatctctg cataacaggg aacaatcagc
      541 cattgagtaa gccaatacac acttagcagg aagtataaaa aaatgctctt cctcatgcct
      601 attcagcgat tataacatac gcacctatat cagctttgtc attaaacaac ctttgacgac
      661 caagcatatc aataggatat tcagcagcta ccagattatc agcattaccc aaagcaggcg
      721 aggagctttg caaggtaaaa tcatagttgt atggtttcgt cgcgttaaac ttaggatctt
      781 ggttagctat tatttccaaa aaattggtat cattgatagt ggcaggacta attttgatta
      841 ctgaattttt aaatacatac actaaaggtt cttcaatttt ttgatttaga tacgaggaac
      901 tgacagatac ttcattcgac caactaccat atactatact attgataaaa ttggcttcga
      961 gtaatggaac tggcatagct tgagaaccaa ggtttatata attagatagc agcaccgaag
     1021 ttgctttacg accaacccaa gggaaggaaa aataattggc aatggtagaa tgcaccacat
     1081 tatattttcc accctgtaac accaaacaag attgccctgc attgtatact acactgttgc
     1141 ttatatgcat aacggtatgc gatgccgtaa ttaaattagt agctgaatta ttaataatcg
     1201 aattagaaat cgcaagagcc aacacatcta aggaagtaga gtcaagaaca atcccttgat
     1261 ttgtattttt gacaacgaca taatttaatt cgttatgtgc actccctttc cgaaaacgga
     1321 taccttgcca ctgattgggc actttatcgt aaggtaaatt atcaaacata ttatccattc
     1381 tatcccctct aaaaacaaca ggtttggatt gtattccttt cgcttttagg gtaccatata
     1441 cttgtaaact ggcatgctta acaaaataaa gagaaacccc ttccgacaag gttaatgttg
     1501 caccctgagc aattgtcaat gtatcgagta tgagatatgg tttttcggct agccaggtag
     1561 tatctgccat tatctgacgt ttagaaacac gatatgcatc ctgtccgtat gcttgcaaca
     1621 ctaccttata cgtatgcgta tttgaacgaa taatgatgga atcttttact ataaagggcg
     1681 taaattggtc agatggatcg acataagcct gcacaaaaat acgcatacta tcatttgctt
     1741 tgagtgaaat atcatgcaaa gcatctgtta gtacaccatt aatatttaga gaataatgag
     1801 tagctcctac taactcaata gcatcgattt taattctatt tttagatgga ttatatatgt
     1861 tgaatatttt ggttgtagat ccaactcccg taaaaagtgt atcaaaagaa acggtatccg
     1921 cagaaatatg tatacgttga ttgggatttg tagaaaacat catatcttcg catgatgaaa
     1981 aacctatcca aaccatacaa ataagaaaaa aaaacgagaa aaaagttcgt tgacgataca
     2041 tacgttattc ttaatgatat agcaattcgt gtacaaaatt acactaatag attgttttat
     2101 tgcaataatc ttgtaaattt gtggaattat taacgaaaaa aactcctttc atatggcaaa
     2161 caagcgtaat cttaaaaaag acattcaatc cattttttct gaattattag tagagtgtta
     2221 caccttatct tatatatttc cacaaattga cattgccaaa gttgatgcaa tagcaacttc
     2281 tatcattgaa aaaagtgata attttgtaaa acgtgcagga aaaccagatg gcaaagacaa
     2341 tccagctatc gtaaaagcct attacaaaaa gctatacaag gatgtagcca atacagtatc
     2401 tgtttttatc gaagatatta acacgttaaa aagcaaataa ataagcatat tttactattc
     2461 gttagcaaca aacttgacac ttaaattacc agagagaaaa aacaaacgaa taaagcattt
     2521 tattttatga aaaaaaatat cagcaaattg ttgttaaaat taatggg
//
LOCUS       DAOPUR010000213         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_42347_length_2566_cov_4.157308, whole genome shotgun sequence.
ACCESSION   DAOPUR010000213 DAOPUR010000000
VERSION     DAOPUR010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42347_length_2566_cov_4.157308"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PKN90_04495"
     CDS             complement(<1..1064)
                     /locus_tag="PKN90_04495"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNZ85571.1"
                     /translation="MMETRDNFTGKIGIVLATVGSAVGLGNIWRFPYEAGQNGGGAFI
                     LIYLLCVFFLGFPLVMAEFAVGVKAKANVVGSFKLLAPNTKWFLVGIMGVVAGFMIMG
                     FYYVVTGWTAEYFWQAIQGNLLHKSSEEYKVMFDSFIASPIKPVIWTVIMIALVHIVI
                     INGVKNGIEKVSKVLMPMLFVLMIILAIHSLMLPNAMQGIRFMFEPKIENVTSYGILT
                     ALGQAFFSLSLGMGCLITYASYFGKDTHIPRIAFQVTLLDSLVAIVAGVVIFPAVFSF
                     GLQPDEGTGLVFVILPTIFQQMPFPIFWSSLFYLLLALAAFTSVISLHEVVTAFVKEE
                     FSYTRKKAVTLVSVAVLVLAV"
     gene            1178..1630
                     /locus_tag="PKN90_04500"
     CDS             1178..1630
                     /locus_tag="PKN90_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HNZ85572.1"
                     /translation="MKERNKRLEAIKEIIAHTTIGSQDNLLESLIDKGFNATQATLSR
                     DLKQLKITKILTDDGIYKYVIPFTNYANKTIGNTREIISIDFSGQLAVIKTRAGYANG
                     IASDIDQQLAELILGTIAGDDTILLIPKEGITRTTITTAIRSFMTIIS"
     gene            1652..2290
                     /locus_tag="PKN90_04505"
     CDS             1652..2290
                     /locus_tag="PKN90_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ85573.1"
                     /translation="MESDTIKIMVANESHYTYIDEILSTIEESSKDRGTGIAKRSPEY
                     VKNKMAEGKAIIALDGEVFAGFCYIESWGNKQYVANSGLIVNPTYRGHGVAKKIKKFS
                     FDLSRQRFPNAKLFGLTSGAAVMKINTELGYVPVTFADLTDDEEFWKGCQGCVNFDVL
                     QRTNRRYCICTAMLYDPNDPNRRNREEKENEAKKQNKKSVITDFINKFKKLK"
     gene            2303..>2566
                     /locus_tag="PKN90_04510"
     CDS             2303..>2566
                     /locus_tag="PKN90_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNZ85574.1"
                     /translation="MKKEKVLAFSGGLDTSFCAMYLAKDKGYEVHTAIANTGGFSPEE
                     LKRIEERAYRLGAKTHATLDVTQQYYDKSIKYMIFGNVLRNGTY"
BASE COUNT          978 a          498 c          400 g          690 t
ORIGIN      
        1 acagctaata ccaacactgc tacagataca agcgtaacag ccttttttct agtataggaa
       61 aattcttctt tcacaaaagc cgtaacaacc tcgtgcaacg aaataaccga agtaaaagcg
      121 gctagtgcta acaacaaata aaatagactt gaccaaaaaa taggaaaagg catttgttga
      181 aaaatggtag gtaatataac aaaaactagt cctgtacctt catcgggctg taaaccaaac
      241 gaaaatacag caggaaaaat tactacacca gccacaattg cgactaaaga atcgagcaaa
      301 gtaacctgaa atgcaatgcg aggaatatgt gtatctttac caaaatatga ggcatacgta
      361 attaaacaac ccatacccaa actcaacgaa aaaaaagctt gtcctaatgc cgttaatatc
      421 ccatacgaag taacattttc aatttttggc tcaaacataa atcgtattcc ctgcattgca
      481 ttaggcaaca ttaacgaatg aatggctaaa ataatcatca acacaaataa cataggcatc
      541 aatacttttg atactttttc aatcccattt ttaaccccat ttattattac aatatgtact
      601 aaagcaatca taatcacagt ccaaataaca ggcttaatag gacttgctat aaaagaatca
      661 aacatcactt tgtactcttc ggacgattta tgtaacaaat tcccttgaat cgcttgccaa
      721 aaatattcag cagtccaacc agtaactacg taataaaacc ccataatcat aaaacctgcc
      781 acaacgccca taataccaac taaaaaccat ttcgtattag gagctagtaa tttaaacgaa
      841 cctaccacat tggcttttgc tttcactccc acagcaaatt ctgccattac caacggaaat
      901 cctaagaaaa aaacgcacag caaataaatt aaaataaatg cacctcctcc attttgacca
      961 gcctcatacg ggaaacgcca aatatttccc aacccaactg ctgaaccaac agtagcaagc
     1021 acaatgccaa tttttcctgt aaaattatcc cttgtttcca tcatgtatct atattatgac
     1081 tgcaaaaata cataatattt acaaaaaaaa tgtataaacg attgcatatt cattttttat
     1141 gtatattttt gcagaataat tgtatattta tttaaaaatg aaagaacgta acaaacggct
     1201 agaagctata aaagaaatta tagcacatac gacaattggc agtcaggata acctccttga
     1261 atcgttgatt gataaaggtt ttaatgcaac tcaagctacg ttatcccgcg atttaaaaca
     1321 actcaaaatt accaagatat taaccgatga tggcatttat aaatatgtaa tcccatttac
     1381 aaattatgca aataaaacaa ttggaaatac gcgagagatt atctctattg atttttctgg
     1441 acaattagca gtaattaaaa cgagagcagg ctatgccaat ggtattgcat ctgatataga
     1501 tcaacaactt gctgaattaa tacttggaac cattgctgga gatgatacaa tattattaat
     1561 ccctaaagaa ggtattactc gcactaccat cactacagca atacgctcat ttatgacaat
     1621 tatttcataa ctaattatat aacttatttg tatggaatct gacactataa aaataatggt
     1681 tgctaacgaa agccactata catacattga cgaaattctt tctaccattg aagagtcgtc
     1741 aaaagaccgt ggcacaggta ttgccaaacg ctcgcccgaa tatgtaaaaa ataagatggc
     1801 agaaggcaaa gcaattatcg cacttgatgg cgaggtattt gctggttttt gttacatcga
     1861 atcgtgggga aacaagcaat atgtagcaaa ctcaggatta atcgtaaacc ctacatatag
     1921 agggcacggc gttgcaaaaa agattaaaaa attctccttt gatttatctc gccaacgttt
     1981 tccaaacgct aagttatttg ggttaacgtc gggagcagct gtaatgaaaa tcaatacaga
     2041 attaggatac gtacccgtta cctttgccga ccttaccgat gatgaagaat tttggaaagg
     2101 atgccagggg tgtgttaact ttgatgtatt acaacgcaca aacagacgct attgtatttg
     2161 tacagctatg ttgtacgacc caaacgatcc taatcgtaga aacagagaag aaaaagaaaa
     2221 cgaagcaaaa aaacaaaaca aaaaaagcgt tattaccgac tttatcaata aatttaaaaa
     2281 attaaaataa attacccgta tcatgaaaaa agaaaaagta ttagcattta gtggtggact
     2341 cgacacttct ttttgtgcca tgtatttagc caaagataaa ggttacgaag tgcataccgc
     2401 aattgctaat acaggtggat ttagccccga agagttaaaa cgtattgaag aaagagccta
     2461 cagattaggt gcaaaaacgc atgctacact tgatgtaacg caacaatact atgacaaaag
     2521 cattaaatac atgatttttg gcaatgtttt gcgtaatggg acctac
//
LOCUS       DAOPUR010000214         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_42394_length_2564_cov_3.140694, whole genome shotgun sequence.
ACCESSION   DAOPUR010000214 DAOPUR010000000
VERSION     DAOPUR010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42394_length_2564_cov_3.140694"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PKN90_04515"
     CDS             <1..996
                     /locus_tag="PKN90_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNZ85575.1"
                     /translation="TVQDKIEFTSDSGASYILLPDDKFSVHALVSFNSPILSNQYANL
                     DSLADFETEIAACRTFVFVREIEPLLKNNLIKGGDLDNAIVIYDQEMPQAELDRLATL
                     MNVPSMKVNNLGYINTKPLVFDNEPARHKLLDIIGDLALIGRPIIGKIIATKPGHAGN
                     TQLAKKISKELRRQETTPPIYNPETAPVMDTNRIKTLLPHRWPFLMVDKILSFKDKEI
                     IGVKNVTCNETFFNGHFPEEPVMPGVLIVEAMAQVGGLLVLNSVDEPARYSTYFLKID
                     NVKFRQKVSPGDTLIFKLILLTEIRRGCANMKGYAFVGKKIVAEAEFMAQIVKNK"
     gene            1038..1808
                     /gene="lpxA"
                     /locus_tag="PKN90_04520"
     CDS             1038..1808
                     /gene="lpxA"
                     /locus_tag="PKN90_04520"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNZ85576.1"
                     /translation="MTNIHPEAQIGANVQIGPFVHIDKNVVIGEGTVIASNVSILEGA
                     RIGKNCKIFPGAVIAAIPQDLKFRGEETIAQIGDNTTIRECVTVNRGTASKGKTIVGN
                     NCLLMAYTHVAHDCVVGNNVILGNATQLAGEVTIHDHAILSGGILVHQFCSIGSHVII
                     QGGSRITKDVPPFITAGRDPLSYAGINTIGLRRRGYSNEQIQSIQDIYRIIYASGLNH
                     TQAIEKLQQEMSPSAEKDEVIHFLQSSERGIIKGYFDK"
     gene            1874..>2564
                     /locus_tag="PKN90_04525"
     CDS             1874..>2564
                     /locus_tag="PKN90_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNZ85577.1"
                     /translation="MDTAENKKSLNFIESIVEKDLKEGKNDGRVQTRFPPEPNGYLHI
                     GHAKAICMDFGIAERFGGICNLRFDDTNPITEDVEYVDAIKHDIQWLGFQWNSIYYAS
                     DYFQQLFELAVRLIKEGKAYVDEQSSEEIAKQKGTPTTPGIESPYRNRPIEESLHLFD
                     KMNAGEIAEGAMVLRAKIDMSSSNMHFRDPIMYRIINTRHHHRTGDKWKVYPMYDYAH
                     GQSDFFEGVTHS"
BASE COUNT          904 a          433 c          480 g          747 t
ORIGIN      
        1 acagttcaag ataaaattga atttaccagc gatagtggtg catcgtatat tttattgcca
       61 gacgataaat ttagcgtaca tgcattagtg tcatttaatt cacctatttt atcaaatcaa
      121 tatgcaaatt tggacagttt agctgatttt gaaactgaaa ttgcagcttg tagaaccttt
      181 gtatttgttc gtgaaataga acccttacta aaaaataacc ttataaaagg tggtgattta
      241 gataatgcaa ttgttattta tgatcaagaa atgccacaag cagaattaga taggttagct
      301 acattgatga atgttccatc catgaaagta aataacctag gttatatcaa tactaaacct
      361 ttagtgtttg acaatgagcc tgcccgtcat aagcttcttg atattatagg agatcttgcc
      421 cttattggtc gacccattat tggaaaaata attgctacta aacctggaca cgcaggcaat
      481 acccaattgg caaaaaaaat aagcaaagaa cttcgtcgtc aagaaaccac acctcctata
      541 tacaatccag aaactgcacc agtaatggat acgaatcgga ttaaaacctt acttcctcat
      601 cgctggccct ttttgatggt tgataaaatc cttagcttta aggacaaaga aattattggt
      661 gtaaaaaatg taacttgtaa cgaaaccttt tttaacggac attttccaga agagcctgtt
      721 atgcctggtg tattaatagt agaagcaatg gctcaagtag gaggattatt ggtattaaat
      781 tcagttgatg agcccgctag atattcaacc tactttttga aaattgataa tgtaaaattt
      841 cgtcaaaaag tatctcctgg cgacaccctc atttttaaat taatattact tactgaaatt
      901 cgtcgcggtt gtgccaatat gaaaggctat gcctttgttg gtaaaaaaat agtagccgaa
      961 gcagaattta tggctcaaat tgtaaaaaac aaataagata acgattttaa cgcaatcaac
     1021 cattaatgat acatagtatg acaaacattc accccgaagc acaaatagga gcgaatgtac
     1081 aaattggacc ttttgtgcat attgacaaaa atgttgtaat tggcgaaggc actgttattg
     1141 catctaacgt tagcatttta gaaggagccc gcatagggaa aaattgtaaa atatttcctg
     1201 gagctgttat agctgctata ccgcaagatt taaaatttcg tggtgaagaa accattgctc
     1261 aaattggaga taacaccacc attcgcgaat gtgtaacggt aaatagaggt actgcctcta
     1321 aaggaaaaac aatcgttggc aataattgtc tacttatggc atacacacac gtagcacatg
     1381 attgtgtggt tggcaacaac gttatcttag gtaatgcaac tcaattagca ggtgaagtta
     1441 ctatacatga tcatgccatt ttaagtggag gtattttggt tcaccaattt tgtagtattg
     1501 gttctcacgt tattattcaa ggaggttctc gcataacaaa agatgtgcct ccttttataa
     1561 ctgcaggaag agacccatta tcatatgcag gaatcaacac gattggatta cgcagacgag
     1621 ggtatagcaa cgaacaaata caatctattc aagatattta tcgtattatt tatgcatctg
     1681 gactaaatca tacacaggca attgaaaaac tgcagcagga gatgtctcca tcagcagaaa
     1741 aagacgaagt aatacacttc cttcagtcct cagaaagagg tattattaaa ggatattttg
     1801 ataaataaaa caaacgaagg aagctgtcaa taaaaaggca gcttttttca aaaaatataa
     1861 cttgattgtt actatggaca cagcagaaaa taaaaagtca ttaaatttca ttgaaagtat
     1921 tgttgaaaaa gacctcaaag aaggtaaaaa tgatggacgt gtgcaaacac gttttccacc
     1981 agaacccaac ggataccttc atataggtca tgccaaagct atttgcatgg attttggtat
     2041 tgctgaacga tttgggggta tatgcaattt acgttttgac gatacaaatc ctataacaga
     2101 agatgtagaa tatgtagatg caataaaaca tgatattcaa tggttaggat ttcagtggaa
     2161 ttcaatttac tatgcttctg attattttca acaattattt gaattagctg taaggttaat
     2221 aaaagaagga aaagcctacg tagacgaaca atcatctgaa gaaatagcca aacaaaaagg
     2281 aacccctact acacctggta tcgaaagtcc gtatcgaaat agacccattg aagaaagttt
     2341 acatttgttt gataaaatga atgctggaga aattgctgaa ggagcaatgg tattgcgtgc
     2401 caagatagat atgtcatcgt caaacatgca ctttcgcgac ccaattatgt accgtattat
     2461 aaacacaagg catcatcatc gtacaggcga caaatggaag gtatatccga tgtacgatta
     2521 cgctcatggt cagtccgatt tttttgaggg agtaacgcat tcta
//
LOCUS       DAOPUR010000215         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_42502_length_2560_cov_3.060679, whole genome shotgun sequence.
ACCESSION   DAOPUR010000215 DAOPUR010000000
VERSION     DAOPUR010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42502_length_2560_cov_3.060679"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKN90_04530"
     CDS             <1..448
                     /locus_tag="PKN90_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNZ85578.1"
                     /translation="ETTLRLANDFSNIIATKEASGNIAQVDAIIKNKPKDFLVISGDD
                     AITFPLITLGASGVISVIGNAFPCEFSKMVRLALAGDYQTALTIHHGFTELIELLFVD
                     GNPAGVKSILSAMGYLENQLRLPLLPTRITTYEKMRNILLNMNIKC"
     gene            477..830
                     /locus_tag="PKN90_04535"
     CDS             477..830
                     /locus_tag="PKN90_04535"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004036.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HNZ85579.1"
                     /translation="MNKLTKEEEHIIVHKGTELPFSGEYVHTKEDGIYSCRRCDTPLF
                     LSDDKFDSECGWPSFDDAIKEAVLEIPDMDGRRTEIVCANCHGHLGHVFEGEGFTEKN
                     VRHCVNSLSIKLMKK"
     gene            830..1309
                     /gene="msrA"
                     /locus_tag="PKN90_04540"
     CDS             830..1309
                     /gene="msrA"
                     /locus_tag="PKN90_04540"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNZ85580.1"
                     /translation="MIEKAYFASGCFWGTEYYFMQLPRIATQVGYMGGLTFAPTYREV
                     CTGDTGHIETVEIVYDNEQVEYETLVKLFFETHNFSQINGQGPDIGSQYVSVIFYTNE
                     KQRDIATYYITLLQTKGYFVATQLRQAEEFWVAEDYHQTYYTKTGGTPYCHKYTKIF"
     gene            complement(1322..2479)
                     /locus_tag="PKN90_04545"
     CDS             complement(1322..2479)
                     /locus_tag="PKN90_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85581.1"
                     /translation="MLKRLKTHLTFLCSFILVAHVYSAQYQGIILDSQSHKAIPYVSI
                     HTSNNNDIYGSMSNDLGEFSISFDFDSLVFQHINYTPYILKKQSYKDTLYLNPLSYTL
                     NEIDVYATESLWIRKKLHLVVKNKKKFYTLPSSIQQDYVYNTYTLSDSSGYAFESKGI
                     LQSNKNEYSILPILSAIKYKDSTAGTDFSNLRRMLHDDFLPRFTSNFIKNNSFRENKG
                     FRHTNPNVVQLFYFSNDNLSDDGYLVIDTLKNVILEAQQNLGTQSNIENNTSSLLRKV
                     AESRGFTYNDWITQTQYIYTEINGSYLLQSCTYKLYMRTTTENKKLSNSYFVSTESTV
                     RLSNPSLTNSVSNSKQFLILPKPYYIVPIYTKTMREEEEKLQRIKRMYKKF"
BASE COUNT          867 a          343 c          460 g          890 t
ORIGIN      
        1 cgaaactacg ttgcgtttgg caaatgattt ttcaaatatc attgccacca aagaagcatc
       61 aggaaatata gctcaagtag atgcgattat taaaaataaa cctaaagatt ttttagttat
      121 ctctggtgat gatgcgatca catttccgtt gataacctta ggtgcttcag gtgttatttc
      181 ggttattggt aatgcttttc cttgcgaatt tagtaaaatg gttcgcttag ccttagctgg
      241 tgattatcag acagctttaa ccattcatca tgggtttact gaattaattg aactattgtt
      301 tgtcgatggt aatccagctg gagtaaaaag tattctatct gcaatgggtt atttagaaaa
      361 ccaattacga ttacctttat taccaacaag gataacaacc tacgagaaaa tgcgaaacat
      421 tcttttaaat atgaacatta aatgctaatt gaaaaattag catttttttt aataatatga
      481 acaaactaac taaagaggaa gaacacatta tagtgcataa aggcactgaa ttacctttta
      541 gcggagaata tgtacatacc aaagaagatg gtatatatag ttgtcgtaga tgcgatacgc
      601 cattgttttt gtctgatgat aaatttgatt ctgaatgtgg ttggccaagt tttgatgatg
      661 ctataaaaga agctgtgttg gaaattcccg atatggatgg tagaagaact gaaattgtgt
      721 gtgcaaattg tcatggacat ctaggacatg tatttgaagg tgaaggtttt actgaaaaga
      781 atgtaagaca ttgtgtaaat tcattatcaa taaaattgat gaaaaaataa tgatagaaaa
      841 agcctatttt gcttctggtt gtttttgggg tactgaatac tattttatgc aactaccaag
      901 aattgcgact caagtaggat atatgggtgg gctgacattt gctccaactt atagagaggt
      961 ttgtacagga gatacagggc acatagaaac agtagaaatt gtttatgata atgaacaagt
     1021 agaatatgag acactcgtta aattattctt cgaaacacat aatttttcgc aaattaatgg
     1081 gcaaggacct gatattgggt cgcaatacgt ttctgtaatt ttttatacca atgaaaaaca
     1141 aagagatata gccacatatt atatcacatt gttgcaaaca aaaggatact ttgtagctac
     1201 acagttacgt caagcagaag aattttgggt agcagaagat tatcatcaaa catattatac
     1261 taaaacagga ggtacaccat attgtcataa gtacacaaaa atattttaga taacactatt
     1321 tttaaaattt tttatacatc ctttttattc gttgcaattt ttcttcttct tctctcatcg
     1381 tttttgtata gattggtacg atgtaataag gtttaggtaa aatcaaaaat tgcttactat
     1441 ttgatacaga atttgttaga ctgggattac tcaaacgaac tgttgattct gttgaaacaa
     1501 aatacgagtt tgatagtttc ttattttcag ttgttgtacg catataaagc ttgtaggtac
     1561 aggattgtag taggtatgag ccgttaattt ctgtatagat atactgggtt tgcgtaatcc
     1621 aatcattgta agtaaaacct cttgattcag ctactttacg caataaagaa gaagtattat
     1681 tttctatatt actttgagta cctaagtttt gttgagcttc gagtattaca tttttcaagg
     1741 tgtctattac caaatatccg tcatcactaa ggttatcatt tgaaaaataa aacaattgta
     1801 caacatttgg attggtatgc ctaaatcctt tgttctcacg aaatgaatta ttcttaataa
     1861 agtttgaagt gaagcgtggt aaaaaatcat cgtgtaacat tcttctcaga ttactaaaat
     1921 cagtacctgc tgtcgaatcc ttatatttta tagcagaaag tattggtaaa attgagtatt
     1981 catttttatt ggattgtaat atacctttgc tctcgaatgc ataaccactt gagtctgata
     2041 aggtatatgt gttatataca taatcttgtt gaatagacga gggtagtgta tagaactttt
     2101 ttttattttt tacaacaagg tgtagttttt ttctaatcca gaggctttct gtggcataaa
     2161 catcaatttc atttagagta taagatagag gattaaggta tagtgtgtcc ttatacgatt
     2221 gtttttttaa aatgtatgga gtataattaa tatgttgaaa tacaagtgaa tcaaagtcaa
     2281 aagagattga aaattcgccc aaatcattac tcatacttcc ataaatatca ttattatttg
     2341 aagtatgtat gcttacgtaa ggaattgctt tatggctttg actatctagt attattcctt
     2401 gatattgagc actatatacg tgtgccacaa gaataaacga gcataagaaa gtaagatggg
     2461 tttttaatct tttgagcata gtaaaatttt tatgctaaaa gtacgaaata gcttagtata
     2521 aaacaaaaaa gtctgtcata attgacaaac tttaaggttg
//
LOCUS       DAOPUR010000216         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_42729_length_2550_cov_3.443687, whole genome shotgun sequence.
ACCESSION   DAOPUR010000216 DAOPUR010000000
VERSION     DAOPUR010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42729_length_2550_cov_3.443687"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PKN90_04550"
     CDS             complement(<1..912)
                     /locus_tag="PKN90_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNZ85582.1"
                     /translation="MELKTELKILLAIVVVFLGIYFLPLESVVFATAIDATLDLSRWY
                     AREHVILCLLPAFLIAGVIAVFVSQGSVIKYFGAKAKRWVAYTVAAVSGTILAVCSCT
                     ILPLFSSIHKRGAGLGPAVAFLYSGPAINILAIILTARILGFEMGVARVIGAVVFSVV
                     IGLIMAFIYRKEEKVKAQEQLSFPDVPEKRPLWQTSLHFFTLVFILVFANWGRPAEGD
                     TTSFWFYLWQYKWHITGVFGLVLVYSMIRILKLSSWQVLLGAVVTVLAAFFIGNPLVV
                     MVVAIAAVSSIALLDKSEDTDNKEWILS"
     gene            complement(966..1679)
                     /locus_tag="PKN90_04555"
     CDS             complement(966..1679)
                     /locus_tag="PKN90_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNZ85583.1"
                     /translation="MEFLQTIVSQSEIPILTAFVLGLMTAISPCPLATNITAIGFISK
                     DMQQKRKVFINGLVYTLGRAVSYTVLGVMLIVLFQQGVSLFKVQRFISLYGMYLLVPF
                     LLVMGVFMLDFIKISLPGFSRMTLKMEEKASSGSAWSSFLMGIVFALAFCPYSGVLFF
                     GALIPLSVSVSAGYALPFVFAIATGLPVVIFAWIFAFSISSVSLFYNKIKVFEKWFRR
                     VVGVLFIAVGLYFAFILFF"
     gene            complement(1679..2074)
                     /locus_tag="PKN90_04560"
     CDS             complement(1679..2074)
                     /locus_tag="PKN90_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85584.1"
                     /translation="MKKTLILAVLALMSVTVVVAQKAEVLYFKADMPCCKARACNNLE
                     ADIKAVVEKNYPKGDVVFKQVRIADEANKALVEKHQAKAQTIVIVGKKKKKKQQVIDV
                     SEVVRGCVRSGDKTELETKLVEAINKAIK"
     gene            complement(2079..2492)
                     /locus_tag="PKN90_04565"
     CDS             complement(2079..2492)
                     /locus_tag="PKN90_04565"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNZ85585.1"
                     /translation="MKQKMSILFVVFLLFVSCAKTASTEQVAQENDVAKVTVYYFHGK
                     QRCPTCVSIQQVAQSAVDTYFANNEDVRFVEVDFSVKKNDALAEKYEVAFSSLIIATA
                     DAYSDITDQAFALATSNPEQLKTIINQEVTKYLNQ"
BASE COUNT          865 a          533 c          427 g          725 t
ORIGIN      
        1 agataaaatc cactccttgt tgtctgtgtc ttccgattta tcaagcaaag caatagaact
       61 tactgctgct attgctacca ccattaccac caagggattg ccaataaaaa aagcagccaa
      121 tacagtaact acagctccta acaacacttg ccacgatgag agttttaaaa tacgtatcat
      181 ggaataaacg agcactaaac caaatacccc tgtaatatgc catttatatt gccatagata
      241 aaaccaaaaa ctcgttgtat cgccttcggc tggtcgcccc caattggcaa ataccaagat
      301 aaataccaag gtgaagaaat gtagcgatgt ttgccataaa ggtctttttt cgggtacatc
      361 aggaaatgat aattgctctt gagcttttac tttttcttct ttgcggtaga taaatgccat
      421 tatcaatccg ataaccacac taaacactac tgctccaatt acacgtgcaa cgcccatttc
      481 gaaacccaaa atacgtgccg taagaataat ggctaagata ttgattgcag gacctgaata
      541 caaaaatgct actgctggac ctaagcctgc tcctcgctta tgaatgctag aaaacaaggg
      601 taatatagta cacgagcaaa cggctaaaat agtgcccgat acggcagcta ctgtatatgc
      661 tacccagcgt ttggctttgg ctccaaaata ctttattacc gaaccttggc ttacaaatac
      721 cgcaataaca cccgcaataa gaaacgcagg caacaaacac aaaataacat gctcgcgtgc
      781 gtaccatcgc gataaatcga gcgtagcatc aatagccgta gcaaaaacaa cactttcgag
      841 aggtaaaaaa taaataccta aaaatactac gacaatggct agtagtattt tcaattccgt
      901 tttgagttcc atagttttat aattatttcg taaaagcacg aaatgaatag gtaaaaaatt
      961 ttattttaga aaaataaaat aaaagcaaaa taaagtccaa cggcgataaa aagaacacca
     1021 actacacgtc taaaccattt ttcgaatact tttattttgt tgtaaaacaa gcttacgctg
     1081 gatatactaa aagcaaatat ccaggcaaaa ataaccacag gcaaaccagt agctatagca
     1141 aacacaaaag gcaaggcata ccctgctgat actgaaacag acaacggaat aagtgcccca
     1201 aaaaataaca caccactata cggacaaaaa gccagagcaa atacaattcc catcaaaaag
     1261 gaactccatg cagaacctga cgatgctttt tcttccattt ttaatgtcat tcgactaaat
     1321 cccggtagcg atattttaat aaaatcgagc ataaaaacac ccattaccaa tagaaaagga
     1381 acaagtaaat acatgccata caaactaata aaacgctgaa ctttaaaaag gcttacaccc
     1441 tgctgaaaca acacaatcag cataacaccc aatacagtat aagacacagc cctacccaat
     1501 gtatatacaa gtccattgat aaataccttg cgtttttgct gcatgtcttt gcttataaac
     1561 ccgatagccg tgatattagt agccaaaggg caagggctga tggcagtcat caaccctagc
     1621 acaaaggctg ttaaaatcgg aatttcagat tgtgaaacaa tggtttgtaa aaactccact
     1681 acttaatggc tttattgatt gcttctacta atttggtttc gagttccgtt ttatcaccac
     1741 tgcgtacgca accacgcact acttctgata cgtcaattac ttgttgtttt ttctttttct
     1801 ttttgcctac aataacgatg gtttgagctt tggcttggtg tttttctacc aaggctttgt
     1861 ttgcctcatc agcaatacga acttgcttaa atactacatc tcctttggga tagttttttt
     1921 ctaccacagc ttttatgtcg gcttctaagt tattacaagc acgagctttg caacaaggca
     1981 tatcggcttt aaaataaaga acttcggctt tttgagctac aactactgta actgacatca
     2041 gtgccaatac ggctaaaata agtgtttttt tcatactttt attgatttaa atacttggta
     2101 acttcttggt ttattattgt ttttaattgt tctggattac tagtggcgag agcaaaagct
     2161 tggtcagtaa tatcgctgta agcatctgct gtagcaatga ttaacgagct aaatgctact
     2221 tcgtattttt cggctaaagc atcatttttt ttgacagaga aatctacttc tacaaaacgt
     2281 acatcttcgt tgttggcaaa atacgtatca acagccgatt gggctacttg ctgaatggaa
     2341 acgcaagtag gacaacgttg cttaccatga aaataatata cagttacttt ggctacatcg
     2401 ttttcttggg ctacttgctc tgtagatgcg gttttggcac acgaaacaaa caagagaaaa
     2461 acaacaaaaa gaatactcat tttctgtttc atagctaggt gcgtttagtt tgttgacaaa
     2521 attgtttgta attgcacttt cgagggaaat
//
LOCUS       DAOPUR010000217         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_42801_length_2547_cov_3.085474, whole genome shotgun sequence.
ACCESSION   DAOPUR010000217 DAOPUR010000000
VERSION     DAOPUR010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42801_length_2547_cov_3.085474"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..528
                     /gene="rpsG"
                     /locus_tag="PKN90_04570"
     CDS             52..528
                     /gene="rpsG"
                     /locus_tag="PKN90_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNZ85586.1"
                     /translation="MRKAKPKKRIVLPDPIFGDVMVTRFVNHLMYDGKKTTAFTIFYT
                     ALENVKGKLPNEEKSSIDIWKKALENITPQVEVKSRRIGGATFQVPTEIRPERKESIS
                     MKNLILYSRKRGGKTMADKLAAEIVDAFNLQGASFKRKEDMHRMAEANRAFAHFRF"
     gene            555..>2547
                     /gene="fusA"
                     /locus_tag="PKN90_04575"
     CDS             555..>2547
                     /gene="fusA"
                     /locus_tag="PKN90_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ85587.1"
                     /translation="MAKGDLSYNRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHD
                     GAATMDWMDQEQERGITITSAATTTSWDYQNEKYKINLIDTPGHVDFTVEVERSLRIL
                     DGAVAAFCAVGGVEPQSETVWRQADKYKVPRIGYVNKMDRSGADFFDVVRQVKEVLGA
                     TPCPIQIPIGAEEKFQGVVDLVKMKAFFWHDETMGAQYHIEEIPANLLSEAKEWREKM
                     LEVVAEFDDVLMEKFFDDPSTITEDEIKAAIRKGTISMQINPMICGSSFKNKGVQTML
                     DAVCAYLPSPLDTPEIIGQDVNDAEKQVVRHPDAKEPLCALAFKIATDPYIGRLCFFR
                     VYSGKIDAGSYVYNTRSGKKERISRIFQMHSNKQNPVDVISAGDIGAGVGFKDIRTGD
                     TLCDENNQMTLESMDFPAPVIGIAIEPKTQKDIDKLGNGLSKLAEEDPTFTVKTDEQS
                     GQTIISGMGELHLEIIVDRLKREFKVECNQGKPQVSYKEAITQTVELREVYKKQSGGR
                     GKFADMMLKVGPVDDNFEGDLQFIDEVKGENIPKEFIPSIQKGFKAAMRNGVLAGYPV
                     DKLKVVVVDGSYHTVDSDQLSFEICAQLAFKNACAKAKPVLLEPIMKMEVITPEESMG
                     DVIGDLNKRRGQVEGMDSSRTGARIVKAKVPLAETFGYVT"
BASE COUNT          872 a          436 c          529 g          710 t
ORIGIN      
        1 ttttgaagac acaatggcaa ccaaatacaa aacggaaaag tttgaaaaca aatgagaaaa
       61 gcaaaaccaa aaaaacggat cgtattacct gatccaattt tcggagatgt aatggttaca
      121 aggtttgtta accacttaat gtatgatggt aaaaaaacca ccgcatttac tatcttttat
      181 acagctttag aaaacgttaa aggtaaatta cctaacgaag aaaagtcgtc tattgatatc
      241 tggaaaaaag ctttagaaaa catcacccct caagtagaag tgaaatctcg ccgtatcggt
      301 ggagctacat ttcaagttcc tactgaaatc cgtccagaaa gaaaagaatc catttcaatg
      361 aaaaacttaa tactctattc tcgcaagaga ggaggaaaaa cgatggctga taaattagct
      421 gccgaaattg tagatgcatt caatctgcaa ggtgcttctt tcaaacgcaa agaagatatg
      481 catcgtatgg ctgaagcaaa cagagctttc gcccattttc gtttttaata tttaacatct
      541 gaagatatac taaaatggca aaaggagatt taagttacaa tcggaatatc ggtatcatgg
      601 ctcatattga tgctggtaaa acaacaactt ccgagcgtat tctattttat acagggttaa
      661 cccacaaaat tggtgaagta cacgatggtg ctgccaccat ggactggatg gatcaagagc
      721 aagaaagagg tattactatt acttctgcag ctactacaac tagttgggat tatcaaaatg
      781 aaaaatacaa aataaatttg attgacaccc caggacacgt tgactttacc gtagaggttg
      841 aacgttcgtt gcgtatccta gacggagctg tagcagcatt ttgtgctgtt ggaggtgttg
      901 aacctcaatc agaaacagta tggcgtcaag cagataaata caaagtgcct cgcatcggtt
      961 atgtaaataa aatggaccgc tctggtgccg atttcttcga cgttgttcgt caagttaaag
     1021 aggtattagg agcaactcct tgtcctattc aaataccaat tggtgctgaa gagaaatttc
     1081 aaggtgtagt agaccttgta aaaatgaagg catttttttg gcatgatgaa acgatgggag
     1141 ctcaatatca tattgaagaa atccccgcta atttgttgag cgaagccaaa gaatggagag
     1201 aaaaaatgct cgaagtggtt gctgaatttg atgatgttct tatggaaaag ttctttgatg
     1261 atcctagcac tatcactgaa gatgaaatca aagcagctat tcgcaaaggt acaataagca
     1321 tgcaaatcaa tcctatgatt tgcgggtctt ctttcaaaaa taaaggtgtt caaactatgc
     1381 ttgatgctgt ttgtgcatac ttgccaagcc cattagatac tcccgaaatt atcggacaag
     1441 atgtaaatga tgctgaaaaa caagtagtaa gacatcctga tgcaaaagaa cctctttgtg
     1501 ctttagcatt taaaattgct accgacccat acataggtcg tttgtgcttc ttccgcgtgt
     1561 attcaggtaa aattgatgca ggttcgtatg tatacaacac acgttctgga aagaaagaac
     1621 gtatttcacg tatttttcaa atgcactcta acaaacaaaa tcctgttgat gtaatctcag
     1681 ctggagatat aggtgcaggt gttggtttta aggatattcg tactggtgat accttgtgcg
     1741 acgagaataa tcaaatgaca cttgagtcaa tggacttccc cgcaccagta atcggtattg
     1801 ctattgagcc taaaacccaa aaagatattg ataaattagg aaatggattg tctaaattgg
     1861 ctgaagaaga tcctactttc actgtgaaaa cagacgagca atcaggtcaa actatcatta
     1921 gtggtatggg tgaacttcat ttagaaatca ttgttgaccg tcttaaacgt gaattcaaag
     1981 tagagtgtaa tcaaggtaaa ccgcaagtgt cttataaaga agcaataact caaaccgttg
     2041 agctacgaga agtgtataaa aaacaatcag gtggtcgtgg taaatttgct gatatgatgc
     2101 ttaaagttgg tcctgttgac gataacttcg aaggagattt acaatttatc gacgaagtaa
     2161 aaggtgaaaa catcccaaaa gaatttatcc cttctatcca aaaaggattc aaagcggcta
     2221 tgcgtaacgg agtgttagct ggctatccag tagataaatt aaaggttgtt gttgtcgatg
     2281 gttcttatca cacagtagac tctgaccaat tatctttcga aatttgtgct cagttagcat
     2341 ttaaaaatgc gtgtgccaaa gcaaaacctg tattacttga accaatcatg aaaatggaag
     2401 ttattactcc agaagaaagt atgggagatg taattggaga cttaaacaaa cgtcgtggtc
     2461 aagtagaagg aatggactca agccgtactg gggctcgtat tgtaaaagca aaagtgcctt
     2521 tagctgaaac ttttggatat gtaacat
//
LOCUS       DAOPUR010000218         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_43255_length_2528_cov_3.224424, whole genome shotgun sequence.
ACCESSION   DAOPUR010000218 DAOPUR010000000
VERSION     DAOPUR010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43255_length_2528_cov_3.224424"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2528)
                     /locus_tag="PKN90_04580"
     CDS             complement(<1..>2528)
                     /locus_tag="PKN90_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNZ85588.1"
                     /translation="KHERLYPVASRCGVFAKTDVQNLLSRNVSKQDIALSIFHAVSLQ
                     VISSLSKGNTIEAPLFLSGGPFAFIPTLKKVFMQQLSINEAQVIDSENRQFIPAWGAA
                     IESNQQTSTLALSALIKKIEQSKQQVYTYNPTALPPLFSDNEHYLRWKKEKESSCVIE
                     TIDIANATEGCYIGIDSGSTTTKIVALDEKKRVFYRFYERNSGSSLDTVQKGLNSLLQ
                     QANEANKNLMVLGSCVTGYGEDLVKNAFPIGLGMVETLAHFTASIHFNPHVSFILDIG
                     GQDMKACFIENGAIKRLDINEACSSGCGSFIETFAHSLGHTAASFSDLCLQSKRPYDL
                     GTRCTVFMNSKVKQALREGAPMADIAAGLGYAVIKNCLNKVLKINDFSTLGKHIMVQG
                     GTFRNIAVLRSLEIETGSNVMITNMPELMGAFGAALFALKNREKQQPTNLQDCVQIRT
                     YTCQTLLCKGCENNCLVTRYQFGKESKFYAGNKCEKVFSNRGNADIKGKNAFDYKYQL
                     LFNRPQAKNPVLKLGIPRALGMYENYPFWHGLFHACGIEVVLSDTSTLKLYEKGAHTV
                     MADNICFPAKLTNGHIVNLMEKKPDRIFLPFVVYENQSDTKTPNSYNCPIVTAYSEVI
                     RSAINPMSSSGIPFDSPSVNFKDKKLLKQACDNYLLSILPHLTKKDLKNAFEYAFKQQ
                     VIFEQTITDYCQTIATKADTEKRFLVVLGSRPYHTDPLVQHKIADVIADFGVDVITED
                     IARMAAKKLPKVHMVMQWAYTNRILKAASWVANKGEHVQYMQLTSFGCGPDAFIIDEV
                     NTLLQQVSKNPTILKIDDINNIGSTRLRIRSVIESLGLSSQAVQ"
BASE COUNT          746 a          519 c          456 g          807 t
ORIGIN      
        1 tttgaacagc ttgacttgac agacccaaac tttctatcac agagcgaata cgcaaacgcg
       61 tagaaccgat attgttgata tcatcaattt tcagaattgt tgggtttttg cttacttgtt
      121 gcaataatgt atttacctca tcaatgataa acgcatcggg tccgcaacca aaagatgtaa
      181 gttgcatata ctgcacatgt tcacctttat tggctaccca gcttgcagct tttaatattc
      241 tattcgtata agcccattgc attaccatgt gcactttggg cagttttttg gctgccatcc
      301 tagcaatatc ttctgtaatc acatccacac caaaatcggc aatcacatca gcaattttgt
      361 gctgcaccaa tgggtcggta tggtagggac gcgagcctaa taccacaaga aaacgcttct
      421 cagtatcagc ttttgttgct attgtttggc aataatcagt aatggtttgt tcaaaaatta
      481 cttgttgctt aaacgcatat tcaaaggcgt ttttcaagtc tttttttgta agatgaggca
      541 atatcgacaa taagtagtta tcgcaagctt gtttcagcag ttttttatcc ttaaaattta
      601 cacttggcga atcaaatgga ataccgctac tagacatagg attgatagca ctacgtatga
      661 cctccgaata ggctgttaca atggggcaat tgtagctgtt tggagttttc gtatccgatt
      721 ggttttcgta caccacaaat ggcaagaaaa tacgatcagg ctttttctcc atcaagttaa
      781 cgatatgtcc gttggttaac tttgcaggga aacaaatatt atcagccatt actgtgtgag
      841 cccctttttc atataatttt aaggtagatg tatccgacaa taccacctca ataccacagg
      901 catgaaacaa gccatgccaa aaaggataat tttcgtacat acccaaggca cgcggaatac
      961 ctagtttcaa cacaggattt ttagcttgag gacgattaaa caaaagttgg tatttataat
     1021 caaacgcatt cttccctttt atatcagcat tacctctgtt tgaaaacact ttttcgcatt
     1081 tatttccagc atagaactta ctttctttgc caaattgata ccgagtaacc aaacaattat
     1141 tttcgcaccc tttacacagt aatgtttgac aggtataggt acgaatttgt acgcaatctt
     1201 gtaagttagt tggttgttgt ttttctctgt ttttcagggc aaacaaagct gcaccaaatg
     1261 cccccattaa ttcaggcata ttggtaatca tcacattgct gccagtttct atttctagcg
     1321 aacgcaatac ggcgatattt ctaaacgtgc caccttgcac cataatgtgt ttgccaagtg
     1381 ttgagaaatc attgattttt aacaccttgt ttaagcagtt tttaataaca gcatatccta
     1441 acccagcggc tatatcagcc attggagctc cctcgcgtaa agcttgtttc acttttgagt
     1501 tcataaaaac ggtacaccgt gtacccaaat cataagggcg tttggattgc aaacacaagt
     1561 cagaaaaaga ggcagcggta tgtcccaacg aatgtgcaaa tgtttcgata aacgaaccac
     1621 accccgaaga acaagcctca ttgatgtcca aacgttttat ggcaccattt tctataaaac
     1681 aggctttcat gtcttgccca ccaatatcta aaataaaact tacgtgggga ttaaaatgta
     1741 ttgaagctgt aaaatgagcc aaggtttcta ccattcccaa tccgatggga aaagcatttt
     1801 ttactaaatc ttcgccatag cctgttacac aacttcccaa gaccattaaa tttttattgg
     1861 cttcgttggc ttgttgcaac aatgagttta agcctttttg taccgtatcc aacgaacttc
     1921 cactattgcg ttcgtaaaac cgataaaata cccgtttttt ttcatccagg gcaacaatct
     1981 tggttgtggt tgaaccgcta tcaattccaa tataacaccc ttcagttgca ttggctatat
     2041 caatggtttc aattacacaa ctactttctt tttctttttt ccacctcaaa taatgctcat
     2101 tatctgaaaa taaaggaggt agtgctgttg gattgtaagt ataaacttgt tgtttcgatt
     2161 gttcaatttt ttttattaat gcagataatg cgagtgtaga tgtttgttgg tttgattcaa
     2221 ttgcagcacc ccatgcaggt atgaattgcc tgttttctga atcaattact tgagcctcat
     2281 tgattgacaa ctgttgcata aacacctttt tcaaggtagg gataaaagca aacggaccac
     2341 cagataaaaa taacggagcc tctatggtat ttcctttgga taaagaagat attacttgca
     2401 acgataccgc atgaaatatg gataaggcaa tatcttgttt gctcacattg cgacttagca
     2461 ggttttgaac atctgttttg gcaaaaacac cgcagcggga ggcaacagga taaaggcgct
     2521 cgtgtttg
//
LOCUS       DAOPUR010000219         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_43568_length_2514_cov_3.344042, whole genome shotgun sequence.
ACCESSION   DAOPUR010000219 DAOPUR010000000
VERSION     DAOPUR010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43568_length_2514_cov_3.344042"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PKN90_04585"
     CDS             complement(<1..998)
                     /locus_tag="PKN90_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ85589.1"
                     /translation="MRTPLLVIWLLFVSSILFGQQKWTLEQCLEHAKVENIQVQQRLI
                     QAENAKINLQQSKQNLLPDLNMYVGQSFSFGRSIDETNTYQNNNVQNTNFSLSSSLNL
                     YDGGRMFYAIEKSKLDFQALLLDVEQEKSTIELQVLLHYFQVSYAKKQVELAENQYAL
                     TSNQLEKTKALIDAGRLPEGEIYQVKAQLSQEELAILQAKHEVNNLLMQFAQLLLIDD
                     VDSFDIEDVEIVAREEYLLPIFENVYTSTIATMPSIRAEQLRLESANQQVRIEKSAYY
                     PRLSMGASYGNGYYNAENRLNLPFSEQWQQNQNIAVTLNLQIPIFNKMSVINRVKQS"
     gene            complement(1003..2085)
                     /locus_tag="PKN90_04590"
     CDS             complement(1003..2085)
                     /locus_tag="PKN90_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ85590.1"
                     /translation="MKKFLKISAIVILVVLVLGTFYMLWKKSQPEKTSFSTTSPIVTT
                     IENKTVATGKVEPRTVVQIKPQISGIITEIYKEAGQQVKAGDVIAKVQIIPDMVSLSA
                     AESRIKRAQIALEKAQIDYDRQKSLFDKGVISQEAYQTATIELKSSQVDLVDAQNNYD
                     LMKEGITRNASNYSNTLIRSTINGMILDIPQKVGSSVIQSNNFNDGTTIATVANMKDM
                     IFVGKIDETEVGKIKVGMPIVLVIGALEGKTFDATLEYIAPQGISDGGAIVFEIKAAV
                     SLSDDVFVRSGYSSNAQIILEKKENILALPESALIFEKDSVFVEVQTKDTLVFDKRAV
                     KLGLSDGINIEVLEGVTQNEKIKLVK"
     gene            complement(2113..>2514)
                     /locus_tag="PKN90_04595"
     CDS             complement(2113..>2514)
                     /locus_tag="PKN90_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ85591.1"
                     /translation="IQLLIWIVGIGTLLAGIIGVSNIMLITIRERTKEIGIRRAIGAT
                     PKVIIGQILVESLVLTVLAGMGGLVLGVWILHFANGLIPADGMFFKNPHIDFGVAIIA
                     TGIMSLFGVLAGIIPARRAIRIKPIDAIREE"
BASE COUNT          777 a          514 c          340 g          883 t
ORIGIN      
        1 gattgtttta ctctgttaat cacagacatt ttattgaaaa tgggtatttg aagattcagc
       61 gttactgcaa tattttggtt ttgttgccat tgttctgaaa aaggtaaatt aagcctgttt
      121 tcagcattat aatacccatt gccataacta gcacccatag ataatcgtgg gtaataagcc
      181 gatttttcta tacgtacttg ttgattagca ctctctaatc gaagctgttc cgcccttata
      241 gaaggcatgg tagcaatcgt acttgtatat acattttcaa atatcggaag caaatactcc
      301 tctcttgcca caatctctac atcttcaata tcaaagctat ccacatcatc aattaaaagg
      361 agttgagcaa actgcatcaa caaattatta acttcatgct ttgcttgcaa tatagcaagt
      421 tcttcttgcg ataattgagc ctttacttgg tatatttcgc cttcaggcaa ccttcctgcg
      481 tctattaaag ctttcgtttt ttcaagttga ttgctagtca aagcatattg attttcagct
      541 aattctactt gtttctttgc atatgacacc tgaaaatagt gcaaaagaac ttgtaattca
      601 attgtacttt tttcttgttc tacatctagt aatagtgcct gaaaatcaag ttttgatttc
      661 tcaatagcat aaaacatcct accaccatca tacaaattta aactacttga taatgaaaaa
      721 tttgtattct ggacattatt attttgatac gtgtttgttt catcaataga tcgaccaaaa
      781 ctaaagcttt gacctacata catatttaaa tcgggtaata aattttgttt cgattgctgt
      841 agattaattt ttgcattttc tgcctgtatc aatcgctgtt gaacttgaat attttctact
      901 tttgcatgct ctaaacattg ctcaagagtc catttttgct gaccaaatag tatactacta
      961 acgaatagca accatataac taatagtggt gttctcatat ttctatttaa caagtttaat
     1021 cttttcattt tgcgttacgc cttccaacac ttcaatatta ataccatcag ataaacctaa
     1081 tttgacagct cttttatcaa atactaatgt gtcttttgtt tgaacctcaa caaatacact
     1141 atctttctca aaaatcaaag cactttcggg caaagccaga atattttctt tcttttctaa
     1201 tataatctgt gcattagaac tataccccga ccgaacaaaa acatcatcag acaatgacac
     1261 agccgcttta atctcaaata caatagctcc accatcgctt atcccttgtg gtgcaatata
     1321 ctctaatgta gcatcaaatg ttttaccttc taaagctcca ataactagca caataggcat
     1381 tccaaccttt attttaccca cttcagtttc gtcaatcttt cctacaaaaa tcatatcttt
     1441 catattggca actgtagcaa tagtggtgcc atcattaaaa ttattacttt gaataacaga
     1501 actacctacc ttttgaggaa tatctaaaat catgccattg attgtggaac gaataagtgt
     1561 attactgtaa ttacttgcat tacgtgttat gccttctttc atcaaatcgt aattattttg
     1621 agcatccact aagtctactt gactagattt aagttcgata gtagctgttt gataagcttc
     1681 ttgagaaata acccccttat caaataaaga tttttgtcta tcataatcta tttgagcctt
     1741 ttcaagtgct atttgagccc ttttaatacg cgattcggca gcacttaatg acaccatatc
     1801 aggaataatt tgtaccttag ctattacatc acctgctttc acttgttgcc cagcctcttt
     1861 gtatatttca gtaataatac ctgatatttg aggtttaatt tgaacaaccg tacgtggctc
     1921 aacctttcct gttgctacgg ttttattttc aatagtggta acaatcggag atgttgttga
     1981 aaagctagtt ttctcaggct gtgatttttt ccacaacatg tagaacgtgc ccaatactaa
     2041 cacaactaat atgacaattg cactaatttt taaaaacttt ttcatatctt tttatagtta
     2101 aaatgttaca ttttattctt ctcttattgc atcaatgggt tttatccgaa tagctcttcg
     2161 ggcaggaata attcctgcaa gcacaccaaa tagcgacata atacctgtag cgataatcgc
     2221 aactccaaaa tcgatatgtg gattcttaaa aaacatgcca tcagcaggaa ttaatccatt
     2281 ggcaaaatgc aatatccaca cacccagaac taagcctccc atacctgcta atacagtaag
     2341 taccaaactt tctaccaaaa tttgaccaat aataactttt ggagtggctc caatggctcg
     2401 cctaattcca atttcttttg ttctctctct aatggtaata agcataatat tgctcacacc
     2461 tattatccca gctaacaagg taccaattcc cactatccaa attaatagtt gaat
//
LOCUS       DAOPUR010000220         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_43672_length_2510_cov_3.112831, whole genome shotgun sequence.
ACCESSION   DAOPUR010000220 DAOPUR010000000
VERSION     DAOPUR010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43672_length_2510_cov_3.112831"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PKN90_04600"
     CDS             complement(<1..195)
                     /locus_tag="PKN90_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85592.1"
                     /translation="MNFLRGIIIMLFAMVSSFHSYSKNGNSANESLWKHYFMPSVGIE
                     LITFRDLATSPLFYDGVGLAG"
     gene            complement(400..957)
                     /locus_tag="PKN90_04605"
     CDS             complement(400..957)
                     /locus_tag="PKN90_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85593.1"
                     /translation="MFKRHYYTPPYILISYIVLLLIFSGCLSIKPSASKSGKKYFETF
                     YVGEEGTQYFIKPISFIDENLYEELSIDITFRYRNIIKDSAIVNFSIRSSDIYKNVDT
                     FKLSNKKIEIQTDRVELIYNEKNNKGYISRHSTKVSLNELKELFNNDDWLITVENSSK
                     ITNYKANRKTIRVINTIRDRVFILM"
     gene            complement(971..1456)
                     /locus_tag="PKN90_04610"
     CDS             complement(971..1456)
                     /locus_tag="PKN90_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85594.1"
                     /translation="MKNLILYLSICITIASCSFKQDSNVLLFNNISFELHEGEKVVKI
                     DPKKKEIFDSYFNNKSIQIPLFKCIKSDNYIIFIGIPFNTSVKELTNYRLKNTLHETF
                     FESDSITYYYKTYKNQEEQVTIYTRNFSDNLVYVLAISDSAKISDTLFNLETLPKRFH
                     K"
     gene            complement(1511..1792)
                     /locus_tag="PKN90_04615"
     CDS             complement(1511..1792)
                     /locus_tag="PKN90_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HNZ85595.1"
                     /translation="MKNLKTLFASFAIVAMMSSCSLTLPVAATSNPVGNKVGTATATG
                     FLGILFFNADASIRTAAKNGGITKISTVDIKQGNVLNLIVTYETIVTGE"
     gene            complement(1879..>2510)
                     /locus_tag="PKN90_04620"
     CDS             complement(1879..>2510)
                     /locus_tag="PKN90_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85596.1"
                     /translation="STVIAIVFLPSIYFGYTLVVKEKFTENATKYISNVSLLDDIYLL
                     KSDIDTKNESISLVYGGNSMNDETRQSIIDKAKDFNLDKAQILVRQGFTFDGAEEHNA
                     ELYKLRLELSHVDTQLAIKQQKIDSIQQQHEMGKIILKEIRTMYPQIQACTFTQGFSH
                     HINKEKEAQQTQIILFSLEGDTISTDNKSTIRNWLKTRLQEDQIRVYYN"
BASE COUNT          771 a          384 c          376 g          979 t
ORIGIN      
        1 tccagccaaa cccacaccat cataaaataa gggcgaagta gccaaatcgc gaaatgttat
       61 caattcaatg cctactgagg gcataaaata gtgcttccaa agagattcat ttgctgaatt
      121 accatttttg ctatacgagt gaaaactgct aaccatagca aataacataa taattattcc
      181 acgcaagaag ttcatttttt tattatttag tgacttaaat gccagtttct gatttcttaa
      241 tatttattca aatttaccag actgtttgta aggaaagaat ttgtagcgaa ggatctatac
      301 ttcgttttta cccttttgtt tcccgttcgg cacaaaatct ttgcttgggt gtcgttgatg
      361 aaattatggg ctgttttaca tacatataaa ttaatcagtt cacattagga taaaaactct
      421 atctctaatt gtgtttataa ccctaattgt ttttctattt gctttgtaat tggttatttt
      481 agatgagttt tcaacagtga ttaaccaatc atcattgtta aataattctt ttaattcatt
      541 taagctaact tttgttgaat gtcgtgatat atatccttta ttattttttt cgttgtatat
      601 aagttctact ctgtcggttt gtatttcaat ctttttattt gaaagtttga aagtatctac
      661 gtttttgtaa atatctgagc ttctaatgct aaaatttact atcgcagaat ctttaattat
      721 gtttctgtat ctaaaggtaa tgtctatact taattcttca tataaatttt catctatgaa
      781 tgaaataggt tttataaaat attgtgttcc ttcttcacca acataaaagg tctcgaaata
      841 ttttttccca gacttactgg cagaaggttt aatagataaa catccgctaa aaattaataa
      901 taatacaatg tacgaaatca gaatgtaagg tggagtatag taatgtcttt taaacatgtt
      961 cttgtgtctt ttatttatga aaccgttttg gaagtgtttc taaattaaat aatgtatcag
     1021 atatttttgc tgaatctgat atcgctaaaa catacactag attatcgcta aagttccttg
     1081 tgtagatggt aacttgctct tcttgatttt tgtatgtttt ataataataa gtaatagaat
     1141 cgctttcgaa aaacgtttca tgtaaagtgt ttttcaggcg ataattagtc aattctttta
     1201 ctgatgtatt gaatggtatt ccaataaaaa ttatataatt atctgatttt atgcatttaa
     1261 ataaaggaat ctgaattgac ttgttgttaa aatacgaatc aaaaatctct tttttcttgg
     1321 ggtcaatttt tacaactttt tcaccttcat gtaattcaaa cgaaatattg ttgaacaaaa
     1381 gcacatttga atcttgttta aaactgcaac tcgcaattgt tatacaaatt gataaatata
     1441 gtattaaatt tttcataagt tatgtttaaa ataagtcaaa gggagtattc cctttgactt
     1501 tgatgaaaaa ttactcacct gttacaattg tttcataggt tacaattaaa ttcagcacat
     1561 tgccttgctt gatgtcaact gttgaaattt ttgtaattcc accattttta gctgcagttc
     1621 tgatgcttgc atctgcatta aaaaataaaa ttcccaagaa tcctgttgcg gttgctgttc
     1681 ctactttgtt tccaacaggg ttgcttgtag ctgctactgg taaagtcaga gagcaacttg
     1741 acatcatagc aacaattgca aatgatgcga aaagtgtttt taaatttttc ataaaaataa
     1801 atttatgttt tttcctactc gtaaggcttt tcggtttcgc cccgtttttt aagtgggttc
     1861 tagactccat ttttcttttt agttataata tactcgtatt tgatcttcct gcaaacgggt
     1921 tttaagccaa tttctaatgg ttgatttatt gtcagtactt attgtatctc cttcaagtga
     1981 aaataaaata atctgtgttt gttgtgcctc tttttcttta ttgatatgat gcgagaaacc
     2041 ttgtgtaaag gtgcatgctt gaatttgagg atacatagtt ctgatttctt ttaaaataat
     2101 tttccccatt tcatgttgtt gctgaatgct atcaattttt tgttgcttaa tagctaattg
     2161 cgtatccaca tggctcaact ccaatcgtaa tttgtatagc tcggcattgt gttcttctgc
     2221 accatcaaag gtaaatccct gacgaactaa aatttgtgct ttatcaagat taaaatcctt
     2281 cgctttatct ataatagatt ggcgtgtttc atcattcatt gaattgcctc catatactag
     2341 agaaatagac tcgtttttag tatctatatc gctctttagt aggtatatat cgtcgagcaa
     2401 acttacgtta gagatgtatt tagttgcatt ttcggtaaat ttttctttca ccaccaaagt
     2461 gtaaccaaaa taaatactag gtaaaaatac aatagcaata actgtagaaa
//
LOCUS       DAOPUR010000221         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_44641_length_2473_cov_3.964433, whole genome shotgun sequence.
ACCESSION   DAOPUR010000221 DAOPUR010000000
VERSION     DAOPUR010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44641_length_2473_cov_3.964433"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..347)
                     /locus_tag="PKN90_04625"
     CDS             complement(132..347)
                     /locus_tag="PKN90_04625"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HNZ85597.1"
                     /translation="MSFLFGILFFILAIGLFVLVFFFSILRNVFRLFTGKQHPEKKEQ
                     ERMARKYVNKKITKQQGEYVDFEEIKE"
     gene            complement(354..1148)
                     /gene="lgt"
                     /locus_tag="PKN90_04630"
     CDS             complement(354..1148)
                     /gene="lgt"
                     /locus_tag="PKN90_04630"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNZ85598.1"
                     /translation="MALATIIWDVNPEAFSIGFITVRWYGLLYAMGFLLGITILGKIF
                     KRENCPEDWADKVFIYMVLAVVIGARLGHVFFYAWDYYKDNLLEIVMIWHGGLASHGG
                     AIAMLIAAWIFSKKVSKKSFLWLGDRLFIPSAMVAGLIRIGNLMNSEIYGHATDLPWG
                     FVFVRDGGTMPMHPTQIYEALAYFALFAFMMYLYWVKDAQKREGLLTGVGFTGIFVAR
                     FFIEFVKNNQEAFEDDLMLNMGQYLSIPFIAIGIWLIIRALRSNKI"
     gene            complement(1180..>2473)
                     /locus_tag="PKN90_04635"
     CDS             complement(1180..>2473)
                     /locus_tag="PKN90_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNZ85599.1"
                     /translation="HQKEADIVAQAGKSGTVTIATNMAGRGTDIKLTPESKAAGGLAI
                     IGTERHESRRIDRQLRGRSGRQGDPGSSVFFVSFEDSLMRLFGSDRIVGIMDRLGLQE
                     NEMMEHRQISNSIERAQKKVEENNFGIRKRLLEYDDVMNSQREVIYARRRHALIGERI
                     GIDIVNMIYDGVENLVNQHDDIQDEEEFRLDCLKLFGVDIQLEEGEIKCTKTIDLVEK
                     VFSLVMANFSRRADKLAEIAYPVIKRVYEEQGKIYENILIPLTDGKKLYNIPVNLKLA
                     YETQAKEIVKSFQKSILLHTIDEAWKEHLRELDELRNSVQNASYEQKDPLLIYKLESF
                     GLFKKMIDAINYKAIAILMRGQIPVTEGETLREAKEERRQDFSKYNTQKSDIMDAGQQ
                     DTREKIHKEPVRVEKKVGRNDLCPCGSGKKYKSCCGKV"
BASE COUNT          793 a          568 c          323 g          789 t
ORIGIN      
        1 gctgtttcgt tttcaagata aagatgggat tgttgtcgca tctattttcc aagtaacacc
       61 tgatggatat ttggaattaa caaaagaaaa cggagatcga caacgttatg cttttaaaga
      121 agttagtttt attattcctt aatctcttca aaatcgacat attcaccctg ttgctttgta
      181 attttcttat ttacatactt acgagccatt cgctcttgtt cctttttctc aggatgttgt
      241 tttcctgtaa ataaacgaaa aacattacgt aatatggaaa agaaaaatac caatacaaat
      301 aaaccaatag cgagtataaa aaataatatc ccaaataaaa aactcattgc atcttatatt
      361 ttattcgaac gtaatgctct aattatcaac catattccaa tggcaataaa tggaatactt
      421 aaatactgac ccatatttaa cattaaatca tcttcaaaag cctcttgatt attctttaca
      481 aattctataa agaaacgagc aacaaatatt cccgtaaatc ctactccagt aagcaagcct
      541 tcacgttttt gtgcatcctt tacccaatat aagtacatca taaaagcaaa cagagcaaaa
      601 taagctaaag cttcgtatat ttgtgttggg tgcatgggca tagtacctcc atcacggaca
      661 aacacaaaac cccatggcaa atcggtagca tgaccatata tctcggaatt catcaaattc
      721 ccaatacgaa tcaaacctgc caccatagcc gaaggaataa acagtctgtc tcctagccac
      781 aaaaaacttt tcttactaac cttttttgaa aaaatccaag cagcaatcaa catagctatg
      841 gcaccaccat gactggctaa tccgccatgc caaatcatca ctatctctaa caaattatct
      901 ttgtaataat cccacgcata aaaaaacaca tgtcccagcc tagcaccaat taccactgcc
      961 aaaaccatgt aaataaacac cttatcagcc caatcttctg gacaattttc acgcttaaat
     1021 attttaccca aaatagtaat acccagcaaa aaccccattg cataaagtaa accataccaa
     1081 cgcacagtta taaatccaat cgaaaaagct tcaggattta catcccatat aatcgtagct
     1141 agtgccattc tctgatgttt ttaaattatt atacttgcat tatactttgc cacaacaact
     1201 cttgtatttc tttccgctac cacaaggaca tagatcatta cgacccactt tcttctcaac
     1261 acgcactggt tctttgtgta ttttttctcg ggtatcttgt tgaccagcat ccattatatc
     1321 cgatttttgc gtattgtatt tactaaaatc ttgtcgtcgc tcttcttttg cctctcttaa
     1381 tgtttctcct tctgtcaccg gaatttgacc acgcatcaaa atagcaatcg ctttgtaatt
     1441 tatagcatca atcattttct taaatagccc aaatgactct agcttgtaga ttaataatgg
     1501 atctttttgc tcataactag cattttgtac tgagttacgt aattcatcta attcgcgtaa
     1561 atgctctttc caagcttcat ctattgtatg caacaaaata gatttttgga acgacttcac
     1621 aatttctttt gcttgtgttt cgtatgccaa tttaagatta acaggaatat tatacaactt
     1681 ctttccatca gttaatggaa ttaaaatatt ctcatatatt ttcccttgtt cttcgtatac
     1741 acgtttgatg acaggatagg cgatttcggc aagtttatcg gctcttcgcg aaaaattagc
     1801 catcaccaaa gaaaatactt tttctactaa atcaattgtt tttgtgcatt taatttcgcc
     1861 ttcctccaat tgaatatcta ccccaaacag tttcaaacaa tccaaacgaa actcttcttc
     1921 atcttgtata tcatcatgtt gatttaccaa gttttctaca ccatcgtaaa tcatattcac
     1981 aatatcaatc ccaatacgtt cgccaataag tgcatgtctg cgacgagcat atatcacttc
     2041 acgctgcgaa ttcatgacat catcatattc taacaaccgc ttacgaatac caaagttatt
     2101 ttcttcgacc ttcttttgag cacgttcaat tgaattagat atctgacgat gttccatcat
     2161 ttcattttct tgcaagccta atctatccat aatccccact atacggtcgg atccaaataa
     2221 acgcatcaac gaatcttcaa aagacacaaa aaatactgaa gaacctggat ctccttgacg
     2281 accagaacga ccacgtaact gacggtcgat acgacggctt tcatgacgtt cggtaccaat
     2341 aattgccaaa cctcccgcag cttttgattc aggagttaat ttaatgtcag taccccgacc
     2401 cgccatattg gtggcaatgg taactgttcc acttttccca gcttgagcaa caatatccgc
     2461 ctctttttga tgc
//
LOCUS       DAOPUR010000222         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_44645_length_2473_cov_3.579404, whole genome shotgun sequence.
ACCESSION   DAOPUR010000222 DAOPUR010000000
VERSION     DAOPUR010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44645_length_2473_cov_3.579404"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1686
                     /gene="rpsA"
                     /locus_tag="PKN90_04640"
                     /pseudo
     CDS             1..1686
                     /gene="rpsA"
                     /locus_tag="PKN90_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275863.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
     gene            complement(1760..>2473)
                     /locus_tag="PKN90_04645"
     CDS             complement(1760..>2473)
                     /locus_tag="PKN90_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ85600.1"
                     /translation="LKAYDEIRKLFSEQQLAKQMGFTAAHFSFNVPGGRCEECQGEGT
                     ITIPMQFMADVVLECEACHGKRFKQDVLEVTYKGVSIADVLDMTVDQAIQFFAATPES
                     TTKKIVKRLQPLQDVGLGYVMLGQSSSTLSGGESQRVKLASFLSNETQEHTIFIFDEP
                     TTGLHFNDIKVLMQAFNTLIDKGHTVIIIEHNIDVIRVADHIIDLGKEGGDKGGYIVF
                     EGTPDEIKNCADSYTGVYL"
BASE COUNT          889 a          478 c          430 g          676 t
ORIGIN      
        1 tcgatgaaca aacgcgaagt agtagtaaac atcggttact aatcagacgg tatcgtgcca
       61 attagcgaat ttcgctacaa tcctgaacta aaagtaggcg ataaagtaga agtatttatc
      121 gaatcgcaag aagacaaaaa aggacaatta gtactttctc acaaaaaagc acgtgcaaac
      181 cgctcttggg atagagtaaa cgaagccctt gaaaaagaag aaattatcaa aggattcgta
      241 aaatgtcgca caaaaggcgg tatgattgta gatgtattcg gtatcgaagc attcttacca
      301 ggttctcaaa tagatgtgaa accaattaga gattacgatg tattcgtaaa caaaacaatg
      361 gaattcaaag tggtaaaaat caaccacgaa ttcaaaaacg ttgtggtatc gcacaaagca
      421 cttatcgaag cagaattaga agctcaaaag aaagacatta tcggcaaact agaaaaaggt
      481 caagtactag aaggtactgt aaaaaacatc acttcttatg gtgtatttat cgaccttggt
      541 ggcgtagatg gtcttatcca tatcacagac ctttcttggg gtcgtatcaa ccatccagaa
      601 gaaattttac aactagacca aaaattgaac gttgtaatcc ttgattttga tgatgacaaa
      661 aaacgtattg ctttaggctt aaaacaactt actcctcacc catgggctgc tcttgacgaa
      721 aacttaaaag taggcgataa agtagaaggt aaaattgtag tagtagccga ttacggtgca
      781 tttatcgaaa ttgctcctgg cgtagaaggt ttaatccacg tgtcagaaat gagctggtcg
      841 caacacttac gcactgctca agatttcgtt aaaattggcg acaaagtaga agctgttatt
      901 ttaacacttg atcgcgacga aagaaaaatg tcattgggta tcaaacaact aaaagaagac
      961 ccatggttaa atatcgaagc taaataccca gtcggaagca aacacaatgc agtagtacgt
     1021 aactttacta actttggtgt atttgtagaa attgaagaag gcgtagatgg tttaatccat
     1081 atttctgacc tttcgtggac taaaaaaatc aaacacccat cagaatttac tcaaattggt
     1141 gcatctatcg aaattcaagt attggaaatt gatgctgaaa accgtcgctt aagccttggt
     1201 cacaaacaat tagaagaaaa tccttgggat gtattcgaaa ccatctttac aaacgactct
     1261 gttcacgaag gtactatcat agaactattc gacaaagggg cagtaatctc tcttcaatat
     1321 ggtgtagaag gttttgcaac tcctaaacat ttagtaaaag aagatggttc gcaagctaaa
     1381 attgacgaaa aactagcatt caaagtaatt gaatttaaca aagatgctaa acgtatcatt
     1441 ctttctcata gccgtatttt cgaagatgct caaaaagcag ctaccgtaac agaagaaaca
     1501 gtagcaactc cagctagcac agaagaaaaa ccaaagaaaa caaaaaaagc gaaagaagaa
     1561 aaagtagaat cagctccatc aatggaaaaa actactttag gcgatattag cggtttggct
     1621 gctttgaaag aagaaatgga caaaaaagaa aagaaagaaa agaaagataa aaaagacaaa
     1681 gaataagcta tctttcacta tataattaaa ggcgatactc ttcatcaagt atcgccttta
     1741 attttttttc aatgtgctat cacaaataaa ctcctgtata cgaatcagca cagtttttta
     1801 tctcatccgg agtgccttca aatacaatat aacctccctt atctcctccc tctttaccca
     1861 aatcaattat atgatcggca actctaatca catctatatt gtgttcaata ataataacgg
     1921 tatgtccttt gtcaataaga gtattaaatg cttgcatcaa tactttaata tcattaaaat
     1981 gcaacccagt agtaggttcg tcaaatataa aaatcgtatg ctcttgagtt tcgttgctca
     2041 aaaatgaggc taatttcaca cgctgactct cccctcccga caaggtactc gacgattgtc
     2101 ccaacataac ataacccaaa cccacatctt gtaatggttg taagcgtttt actattttct
     2161 ttgttgtaga ttcaggcgtt gctgcaaaaa attgaatggc ttgatcaact gtcatatcca
     2221 acacatcagc aatgctaact cctttgtatg ttacctccaa cacatcttgc ttaaaccgct
     2281 taccatgaca cgcctcacac tctagtacca catcagccat aaactgcata ggtatcgtta
     2341 tcgtaccttc tccttgacac tcttcacatc tacctccagg tacattaaac gaaaaatgtg
     2401 cagcagtaaa acccatttgt ttggctaatt gttgttctga aaataatttt cttatttcat
     2461 cataggcttt cag
//
LOCUS       DAOPUR010000223         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_45044_length_2458_cov_2.601748, whole genome shotgun sequence.
ACCESSION   DAOPUR010000223 DAOPUR010000000
VERSION     DAOPUR010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45044_length_2458_cov_2.601748"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PKN90_04650"
     CDS             complement(<1..948)
                     /locus_tag="PKN90_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNZ85601.1"
                     /translation="MEATKSLNKICALKKRIRVIQGGQGAGKTWAILIILIDFAFTNP
                     NKEIFICSDELSKMRITIIKDFIKIMRLFERFDRSQWVDGTLYRFENGSFIKFIGLDK
                     ADIGKGLRSDVLFINEANKTNFETYREITSRAKNVYLDFNPNNEFWVHEEVVNRSDCD
                     FLKLTYKDNEYLSIEEKTEIELYYKKGYDSNGNVINDYWANMWQVYGLGNIGRLQGSI
                     YQNWVVGSFPELPHAYGLDFGVKDPDALVKCAIDRTNMKIYLHEEIYQNGQSTKELYE
                     SIKSRITANSLIVADSSGARHILDLKNYGLNIESVHKLRV"
     gene            complement(935..1303)
                     /locus_tag="PKN90_04655"
     CDS             complement(935..1303)
                     /locus_tag="PKN90_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85602.1"
                     /translation="MAYDKKKIYEQAKEQIVKNNLFFVEDIVAFLPCGKSYFYDTFTL
                     DSDEMHTLKEMLDNNKIKTKSSIRAKLYKSEKAAELLALYRLIATPEEHRLLNQQYIE
                     NKNTHEIKGLITTNPLNGGD"
     gene            complement(1303..>2458)
                     /locus_tag="PKN90_04660"
     CDS             complement(1303..>2458)
                     /locus_tag="PKN90_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HNZ85603.1"
                     /translation="LGGNMRLKALQELKYKEIPDTWIMLADEWSEEKRKEFTIKDNVS
                     FGEWDWDRLANVWNVEELTEWCLDIPNFDFDEVEDVKEQNKKLSDRFIIPPFSVLDTR
                     CGEWQDRKRYWLSIGIESELGRDSKSIKFSKMAEDLAWAGQKTGISIFDPVLCELVYR
                     WFNVPNGKILDCFAGGSVRGIVAAKLGYEYLGNDLRSEQIEANRINAKEVLQDSEIYP
                     TWTCGDSLHIDTIAKGYEADLLFSCPPYAGLEVYSDKKEDISNMPYKDFIAVYREIIR
                     KSCSLLKDDRFAVFVVGDVRDKKGFYQNFVSDTIMAFWNCGVILYNEMILVNQIGNLP
                     MRAGRYFASGRKVGKQHQNVLVFYKGNPKNIKSNYPELDLSYIENEIESE"
BASE COUNT          709 a          519 c          329 g          901 t
ORIGIN      
        1 tacccgcaac ttgtgtacac tctcaatatt taacccgtaa tttttcaagt cgagtatatg
       61 cctcgctccc gaactatcag caactattaa actattcgcc gtaattctcg attttataga
      121 ttcgtatagt tcttttgtgg attgcccatt ttgatatatt tcttcatgta ggtatatttt
      181 catattagtt ctatcaatag cacattttac aagtgcatcg gggtctttta caccgaagtc
      241 taacccgtag gcgtgtggta attctggaaa tgaaccaact acccaatttt gatatataga
      301 gccttgtaaa cgtccaatat tcccaagccc gtaaacctgc cacatgttcg cccagtagtc
      361 attaattaca ttaccgttgg agtcatatcc ttttttgtaa taaagctcta tttctgtttt
      421 ttcttcaatc gacaaatact cattgtcttt gtatgttagt ttgagaaaat cacaatcgga
      481 acggtttaca acttcctcat gtacccaaaa ttcattattc ggattaaaat ctaaatatac
      541 attttttgca cgtgaggtta tttctcggta agtttcaaag tttgttttat ttgcttcatt
      601 tatgaacaac acatcagatc ttaaaccttt tcctatatcg gctttatcga gtccgataaa
      661 cttaataaac gaaccatttt caaaacgata taaagtaccg tcaacccatt gcgacctatc
      721 gaaccgctcg aataaccgca tgatttttat aaagtctttg attatcgtta ttcgcatttt
      781 gctcaactcg tcggagcaaa tgaatatttc tttgtttgga ttggtaaatg cgaagtcaat
      841 tagaattata agtattgccc aagttttacc ggcaccctgc ccaccctgta taactctaat
      901 tctttttttt aaagcacata ttttattaag tgatttagtc gcctccattt aacgggttcg
      961 ttgttatcag accttttatt tcgtgagtat ttttgttttc tatatactgt tgattcaaca
     1021 atcgatgttc ttcaggagtt gcaataagtc tatataatgc caataattcc gctgcctttt
     1081 ccgacttata caattttgca cgtatgcttg attttgtttt aattttgtta ttgtcaagca
     1141 tttcctttag agtgtgcatt tcgtccgaat ctaatgtaaa agtgtcgtaa aaatatgact
     1201 taccacaggg caaaaatgct acaatgtcct caacaaagaa tagattgttt tttacaatct
     1261 gttcctttgc ttgttcgtat attttctttt tatcgtaagc cattattcgc tttctatttc
     1321 attttcaata taacttaaat ctaattcagg gtagtttgat tttatgtttt ttggattgcc
     1381 cttgtaaaat accaaaacat tttggtgctg ttttcccact tttcgacctg atgcaaaata
     1441 cctacctgcc ctcattggca aattacctat ttgattaact aaaatcattt cattatataa
     1501 aataacgcca caattccaaa acgccattat tgtatcagat acaaagtttt gataaaatcc
     1561 ttttttatca cgtacatcac ctacaacaaa tacggcaaac ctatcatctt ttaataggct
     1621 gcaagatttg cgtataattt cacgatatac ggcaataaaa tctttatatg gcatattgct
     1681 aatatcttct tttttgtcgc tgtaaacctc taagcctgca tacggtggac aactgaataa
     1741 taaatcagct tcatatcctt ttgcaatggt atcaatatgt aacgaatcgc cacaagtcca
     1801 agttggatat atttctgaat cctgcaatac ttcttttgcg tttattctgt tagcttctat
     1861 ttgttcgctt cgtaaatcat tacctaaata ctcataacct aatttagcag ctacaattcc
     1921 acgaacagaa cctccagcaa aacagtcaag tattttacca tttggaacat taaaccatct
     1981 ataaactaat tcacaaagta ctggatcgaa aatagatatt cctgtttttt gaccagccca
     2041 tgctaaatcc tctgccattt ttgaaaactt aatactttta gaatccctgc ctaattcgct
     2101 ttcaattcca atcgataacc aatatctttt cctatcctgc cattcaccac atcttgtatc
     2161 aagtacagaa aatggcggta taataaacct atcagatagt tttttatttt gttcttttac
     2221 atcttcgact tcgtcaaaat caaaattcgg tatatccaag caccattctg taagctcttc
     2281 aacattccat acattcgcca acctgtccca atcccattcg ccgaagctaa cgttatcttt
     2341 gattgtaaac tcttttcgtt tctcctcact ccattcatct gctaacatta tccaagtgtc
     2401 tggaatttct ttgtatttca attcctgcaa cgctttcagt cgcatatttc caccaaga
//
LOCUS       DAOPUR010000224         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_45147_length_2454_cov_3.072113, whole genome shotgun sequence.
ACCESSION   DAOPUR010000224 DAOPUR010000000
VERSION     DAOPUR010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45147_length_2454_cov_3.072113"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKN90_04665"
     CDS             <1..305
                     /locus_tag="PKN90_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNZ85604.1"
                     /translation="LCGGDMSFTSALTFDFEVFSAAQNRWLEVSSVSNFESFQANRLK
                     CRYKGDDKKTSLCHTLNGSALALPRIVAALLENNQTEEGIVIPKALVPFTGFTIIS"
     gene            477..1550
                     /gene="corA"
                     /locus_tag="PKN90_04670"
     CDS             477..1550
                     /gene="corA"
                     /locus_tag="PKN90_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNZ85605.1"
                     /translation="METLKPLRSVSILNRKKNDPSNYIFTGNSSTKNIDIQLFTYNEE
                     ECVENKIMLLDSIPTFEKNKKNYWLNIHGLNNPENIASICEQQGIHNLVIQDILDVNQ
                     RPKFQEFEHFSFLTLKSIVPSEKNIITEQISFVFGTNFLLSFQERKADFFEHLRYRLR
                     ESKGKLRERGADYLLYAMLESTLDNYFKTINKLDEEVEKLNFSDTTKEPSPNALAIIE
                     GHKKFVHYIKKSILPIKEFTQIVERGDCNYIEQRHVKYFLEIKDLCLTLIDSCDMILA
                     SLESATNLFFSVQGHRMNQVMKTLTIVATIFMPLTFIAGIYGMNFVHMPELEWKYGYY
                     GIWGIMIVLFCAMVLYFRAKKWF"
     gene            1823..>2454
                     /locus_tag="PKN90_04675"
     CDS             1823..>2454
                     /locus_tag="PKN90_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNZ85606.1"
                     /translation="MSLQKDILELREKKAIYEKGGGDTAIEKQMAMGKLTARQRILAL
                     LDEDSFQEYDLFVEHEERNFGMDKKTLHGDGVITGIGTMFGEPVCIYAQDFTVMGGSL
                     GLKHARKITKIMDHALKMKVPCIGINDSGGARIQEGIGALAGYGEIFYRNTLASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVENISKMFITGPSVIESVLGEVISQ"
BASE COUNT          820 a          322 c          436 g          876 t
ORIGIN      
        1 gcctttgtgg tggagatatg agttttactt cggcattaac tttcgacttt gaggtatttt
       61 cggctgctca aaaccgttgg ttagaagtaa gttctgtatc caactttgaa agttttcaag
      121 caaatagatt aaaatgtagg tacaaaggag atgataaaaa aactagttta tgtcatacac
      181 ttaatggtag tgcacttgcg ttgcctcgta tcgtagctgc attgttagaa aataaccaaa
      241 ctgaagaggg tattgttatt ccaaaagcct tagttccatt tactggtttt actattattt
      301 cgtaacaaat actcatttct tactgtatat ttaaaatagg aggctttttg tttcctattt
      361 tttttgttca gacatttaat ggcaaattta cgatgaaaat ataaaaaatg tagttatctt
      421 cgaagtctat aagtaaaaaa tgtatgtaaa gaatatataa gtaactagca aaaaacatgg
      481 aaactctcaa accattacga tcggtttcta ttttaaatag aaagaaaaac gacccaagta
      541 actatatttt tacaggaaat tcgagtacta aaaatattga tattcagtta tttacttata
      601 atgaggaaga atgtgtagaa aataaaatta tgttactaga tagtattccg acgtttgaaa
      661 aaaataaaaa aaattactgg ttaaatatac atggattaaa taatccagaa aatattgcgt
      721 ctatttgtga acaacaaggg attcataacc tagttattca ggatatttta gatgtaaatc
      781 aacgaccaaa atttcaggaa tttgaacatt tttctttttt aacattaaaa tcaatagttc
      841 cttcggagaa aaatatcata actgagcaaa ttagctttgt atttggtacc aactttctat
      901 tgtcttttca agagagaaaa gcggactttt ttgagcatct tcgatataga ctcagagaaa
      961 gtaaaggtaa attacgagaa cgaggtgcag attatttgct ttatgctatg ctagaatcaa
     1021 ctctcgataa ctacttcaaa actattaata agctagatga ggaagtggaa aaattaaatt
     1081 tttcggatac cacaaaagaa ccatccccca atgctcttgc tataattgaa ggacataaaa
     1141 aatttgttca ttatataaaa aaatctatat tacctataaa agaatttaca caaattgttg
     1201 aacgtggtga ttgtaactat atagaacaaa ggcacgtaaa atattttctt gaaattaaag
     1261 atttgtgttt aacattgatt gatagttgcg atatgattct tgcttcgctt gaaagtgcaa
     1321 caaacttatt tttctcagtt caggggcatc gtatgaatca agttatgaaa accttaacca
     1381 tagtggctac aatctttatg ccacttacat ttattgcagg tatttatggt atgaattttg
     1441 tgcatatgcc agaacttgag tggaagtacg gatattatgg catttggggt attatgattg
     1501 ttttattttg tgctatggta ctctatttta gagctaaaaa gtggttttag atatatcttt
     1561 ttatacttac gtaatttgtt attcttcatt ctcgtatttt aaattttatc ttcttaaaaa
     1621 cttttgtttt ttgtttcttt tttaattttt aagggttaat ttacacattt tatacttttt
     1681 tgtgtattgt aagtgtttga taatcaatgg tgggtttttt ttgttgatat ttttatctac
     1741 tttatcccgt tcacaaataa aagatttgct taactttgcc atgtgaatta aaatttcaat
     1801 tattcattaa aaagtaacaa gtatgtcact acaaaaagac attttagaat tacgtgagaa
     1861 aaaagccatc tatgaaaaag gtggtggcga cacagctatc gaaaaacaaa tggctatggg
     1921 gaaattaaca gcccgccaac gtattttagc tctattggat gaagattctt ttcaagagta
     1981 tgatttgttt gttgagcacg aagagcgtaa ttttggcatg gataaaaaaa cactccatgg
     2041 tgatggtgtt attacaggta ttggtaccat gtttggtgag cctgtttgta tttatgcaca
     2101 agattttaca gtaatgggag gttctttggg tttaaaacat gctcgtaaga ttaccaaaat
     2161 tatggatcat gcattgaaaa tgaaagttcc ttgcataggt attaacgatt caggtggagc
     2221 tcgtattcaa gaaggtattg gtgcattagc aggatatggt gagatttttt atcgcaatac
     2281 attagcttct ggtgtaattc ctcagatttc tgtaatttta ggaccatgtg caggtggagc
     2341 agtgtattcg ccagctctta ctgattttgt atttgtagtt gaaaatattt caaagatgtt
     2401 tattacaggt ccaagtgtaa tcgaatcagt attaggagaa gtaatttctc aaga
//
LOCUS       DAOPUR010000225         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_45315_length_2448_cov_3.585040, whole genome shotgun sequence.
ACCESSION   DAOPUR010000225 DAOPUR010000000
VERSION     DAOPUR010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45315_length_2448_cov_3.585040"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PKN90_04680"
     CDS             complement(<1..227)
                     /locus_tag="PKN90_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85607.1"
                     /translation="MMTATIHSFLPHQMDAIVLADGAFPTHPDLVALLLTHERVICCD
                     GAAEKLMQMGRIPSCVIGDLDSISAITKAQLG"
     gene            complement(224..820)
                     /gene="pnuC"
                     /locus_tag="PKN90_04685"
     CDS             complement(224..820)
                     /gene="pnuC"
                     /locus_tag="PKN90_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNZ85608.1"
                     /translation="MDYLQQHWLEWFGVITGLLFLYLEIKQYPIMWIVGIITSAVYIV
                     VYLQASFYAYGLLNVYYVAISVYGLWLWVRGDNRNKTETTSSQHYRHIKGRLAVFVAA
                     ITIILCVLIYWALVSFTDSVMPSAEAIATALSIVATWMLAKRFIEHWFVWIVVNIVSV
                     TMFAMQGLYPTALLFFCYVFLSSIGYLQWRKFSNYEVL"
     gene            complement(841..>2448)
                     /locus_tag="PKN90_04690"
     CDS             complement(841..>2448)
                     /locus_tag="PKN90_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ85609.1"
                     /translation="DLSSYFVQGGYFGKHTVIKLLSFGGKEQTYHAWDGVPKDKLQSD
                     RTYNPSGYMGDDVFGNPMYYDNQTDNYQQTHYHLLLNHSFSSSLSLNLALHYTKGKGY
                     YEEYKTDRKLKEYGLSNYVFESETITKSDLVRQKHLDNDFGGAVFSLDYAVDKLSVSW
                     GGGANYYNGDHFGKITWIKNYAGDAAFMPGKEYYASVGEKTDANTYLKANYAVTEAIN
                     LYGDVQYRYINYQTYGTSDRWDWMNNQMQVLNVNEDFHFFNPKAGIFYAIDEQNSAYA
                     SVAVGHREPTRNNYKDAELTANPTFERLINYELGYSYKHATYAFGANAYYMDYKNQLV
                     LTGKVNEIGEALTSNTPESYRMGIELTAGVQITPWLQWNGNLTVSENIIKKYTEYVDL
                     YDENWDWTSQVATYLGDTPISFSPNLIANSVFSINHNGWSAALQSSYVGKQYIDNSGS
                     DERSLSAYFVNNARLGYTFTLKGMKELGIGILCNNLLNEQYESNAWVYSYYQQPDMNN
                     PYKDRYADFGYFPQAGRNLMLNMVLKF"
BASE COUNT          783 a          518 c          387 g          760 t
ORIGIN      
        1 cctaattgag ctttagttat tgctgaaata ctatccaaat cgcctattac acaagaggga
       61 attctaccca tttgcatcaa tttttctgct gctccatcac aacaaataac cctttcgtgg
      121 gttaacaaca aagctaccaa atcaggatgt gtagggaatg caccatcggc taacacaata
      181 gcatccatct gatgaggtaa aaaagaatga atggtcgctg tcatcataag acttcatagt
      241 tagaaaattt tctccattgt aaatacccaa ttgaagataa aaacacataa caaaagaaca
      301 ataaagccgt tgggtacaag ccttgcatag caaacatggt aacagaaact atatttacca
      361 ctatccacac aaaccaatgt tcgataaatc gttttgccag catccatgta gcgactatac
      421 taagagcagt agcaatggct tctgccgatg gcataactga atcggtaaag gataccaaag
      481 cccaataaat aagaacacat aagatgatag ttattgccgc aacaaaaaca gctaatcgtc
      541 cttttatatg cctataatgt tgcgaagagg tagtttctgt tttattccga ttatcgccac
      601 gtacccacag ccatagtcca tacacactta ttgcaacata atacacatta agtaagccat
      661 aagcataaaa actcgcctgc aaatatacca caatatatac agcagaagtt ataattccca
      721 ctatccacat tatcggatac tgctttattt cgaggtataa aaagagtagt ccggtaataa
      781 caccgaacca ctctaaccaa tgttgttgca aataatccat gtacaaagtt aatgatttta
      841 ctaaaacttg agtaccatat tcaacattaa gttacgccct gcttgtggaa aatagccgaa
      901 atcagcatac ctatctttgt atggattatt catatctggt tgttggtaat acgaatacac
      961 ccaagcatta ctttcgtatt gttcattcaa cagattatta cataaaatac caatacctaa
     1021 ttctttcatt ccttttaagg tgaaagtgta accaagtcgt gcattgttta caaaatatgc
     1081 tgataaggag cgttcatcac tacctgaatt gtcaatgtat tgcttgccta cataacttga
     1141 ttgcaatgct gctgaccatc cgttatggtt gatactaaac acactattgg caattagatt
     1201 aggtgaaaat gaaatagggg tatcgcccaa ataagtagca acttgcgatg tccaatccca
     1261 attttcatca tacaaatcta catactcagt atactttttg atgatatttt cacttacagt
     1321 taggttaccg ttccattgta gccaaggagt aatttgcaca cccgccgtaa gttctattcc
     1381 catacggtaa ctctctggtg tattgcttgt taaggcttct cctatttcgt ttaccttgcc
     1441 agttaatacc aattgatttt tgtaatccat gtaataggca ttggctccaa aagcatacgt
     1501 agcatgtttg taagaatacc ctagctcata attaatcaag cgttcaaaag ttggattggc
     1561 tgttaactca gcatctttgt agttgtttcg agtaggctct ctatgcccta cagccactga
     1621 tgcataagca gaattttgct cgtcaatggc ataaaatata cctgcttttg gattaaagaa
     1681 atgaaaatct tcgtttacat tcaatacttg catctggtta ttcatccaat cccaacgatc
     1741 gctagttccg tatgtttggt aattgatata gcgatactgt acatctccat acaaattgat
     1801 agcttctgtt acagcataat ttgcttttaa ataagtattt gcgtcagttt tctcccctac
     1861 gctagcataa tattctttgc caggcataaa cgcagcatcg ccagcataat tttttatcca
     1921 tgttattttt ccgaaatggt cgccattata ataattagct cctccacccc atgacacaga
     1981 caatttatcc acagcataat cgagcgaaaa tacagctcca ccaaaatcat tatccaagtg
     2041 tttttgacgc actaaatcac ttttagtaat tgtttcactc tcaaacacat aatttgacaa
     2101 accatactct ttcaattttc tatctgtttt atattcctcg taataccctt ttcctttggt
     2161 atagtgcaat gccaaattga gcgataagga agaactaaaa gaatgattta acagcaagtg
     2221 atagtgcgtt tgttggtaat tgtctgtttg attgtcataa tacatcggat taccaaatac
     2281 atcatcgccc atatagccac ttgggttgta ggtacggtct gattgtaact tatctttcgg
     2341 cacaccatcc cacgcgtgat aggtttgttc tttgccacca aacgacaaca gtttgattac
     2401 cgtgtgttta ccgaaatacc ccccttgtac aaaataagaa gataaatc
//
LOCUS       DAOPUR010000226         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_45584_length_2438_cov_5.852287, whole genome shotgun sequence.
ACCESSION   DAOPUR010000226 DAOPUR010000000
VERSION     DAOPUR010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45584_length_2438_cov_5.852287"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1194
                     /locus_tag="PKN90_04695"
     CDS             172..1194
                     /locus_tag="PKN90_04695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNZ85610.1"
                     /translation="MNFEKKYIDKVIQGDSLSILKEMPNDMVDLVVTSPPYFQQRDYG
                     NIGIGNEKTVEEYIRNLILIFEECVRVTKPTGSIVFNVGDKYINGNLLLVPYRFAIEA
                     QKNKDLRLVNELTWVKVNPTPKQDTRKLISSKEPFFIFAKSNDYYFDKNAFLEYKDMF
                     LNGNQTRNRKDVGKRYFELIENSNLSVEEKRQAKKELTEVILEVKSGKLESFRMKIRD
                     IHALPYGGQAGGRLTQIQNKGFTIIKIYGNSIRKDIIESTVETIKGNQHPAVYPEFVV
                     QELIKLLTPQKAIVLDPFLGSGTTAVVAKKLQRNYIGIEIYDKYVEYAKERINEISQP
                     QMQLFL"
     gene            1191..2315
                     /locus_tag="PKN90_04700"
     CDS             1191..2315
                     /locus_tag="PKN90_04700"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease, SacI family"
                     /protein_id="HNZ85611.1"
                     /translation="MKETTILENILKKSTKKNVKINQTTSDNVRTLVKHIGSNKSLVS
                     ALVTSCLKKIIDPSQDIRLHRTDFNGGYSARSLDTNVTVPFFKLHFPKYANKESAFLT
                     LATRERIKWTIQEGEGLKIRNKEVKSAFLQLLDLVENKGKSEECLLSIFKELTLLSEK
                     ETLIFDETITALGFSNVLNINKVIDMLVTHFSLKLSSRLPVIAIYTIYQELIKNVKRY
                     NGKILKPLNVHTSADKHGYGDVEIWNKNNTPFEMVEIKHNIAIDRNLIFDVVKKSKNT
                     TIKRYYVLTTFTDSFLSKNEELYINNFILKIKQDTDIDVIANGIIPSLKYYLRFIDDY
                     KAFIKSYTKNLIADAKNSTEIKDFHLTTWQEILKKNNIDK"
BASE COUNT          969 a          318 c          380 g          771 t
ORIGIN      
        1 tctcaaacta taatatttat caacccaaaa cacaacaatt aacacttttt taattaagga
       61 atgtaaattt actaaaactc tcacattaaa tgaataaagc acaaataata tctctactaa
      121 aatgaaaact gtctattgaa aatataacat aaaattgatt atatttgcaa tatgaatttt
      181 gaaaaaaaat atatagataa agtaattcag ggggactctc tgagcatatt gaaagaaatg
      241 cctaacgaca tggtggattt agttgttaca agtcctcctt attttcaaca acgcgactat
      301 ggtaatattg gaattggtaa cgaaaaaaca gttgaagaat atattagaaa tttaatttta
      361 atatttgaag aatgtgtacg agttactaag ccaactggga gcatcgtttt taatgttgga
      421 gataaatata taaatggaaa tttgttatta gtcccttatc gttttgctat tgaggctcaa
      481 aaaaataagg atttaagact tgtgaatgaa cttacttggg taaaagtaaa tccaacacct
      541 aagcaagaca caagaaaatt aatttcttcc aaagaaccat tttttatttt tgcaaaaagc
      601 aatgactatt attttgataa aaatgcattt ttagagtata aagacatgtt tttgaatggt
      661 aatcaaacaa gaaatagaaa agatgttggc aaaagatatt ttgagttgat tgaaaattca
      721 aatctaagtg tcgaagaaaa acgacaagca aaaaaagaac ttacggaagt tattttagaa
      781 gtaaaatctg gtaaattaga gagttttcgc atgaaaatac gcgatattca tgcattgcct
      841 tatggtgggc aagctggtgg taggctcaca cagatacaaa ataaaggctt tacgataata
      901 aagatttatg gtaattctat acgaaaagac attatcgaat caaccgtaga aactattaaa
      961 ggcaatcaac accctgcggt ttatcccgaa tttgttgtgc aagaattaat aaaattactt
     1021 acacctcaaa aagctattgt acttgaccca tttttaggat cgggtactac agctgtggtt
     1081 gctaaaaaat tgcaacgtaa ttatatcgga attgaaattt acgataaata tgttgaatat
     1141 gctaaagaaa gaataaacga aatatcgcaa ccacaaatgc aattattttt atgaaagaaa
     1201 caacaatttt agaaaatatt ttaaaaaagt caactaagaa aaatgtcaaa ataaatcaga
     1261 ctacttctga taatgttcga accctcgtaa aacatattgg tagcaacaaa tctcttgttt
     1321 ctgcacttgt tacgagttgt ttgaaaaaaa taatagaccc ttctcaggat atacgtttac
     1381 atcgaacaga ttttaatggt ggttattcag ctcgcagttt agatacgaat gttactgtcc
     1441 cattctttaa attgcatttc cctaaatatg caaataaaga atccgctttt ttgactttag
     1501 caacaaggga aagaataaaa tggacaatac aagaaggaga aggactgaaa attaggaata
     1561 aggaggtgaa atcagctttt ttacagttgc ttgatttggt tgaaaataaa gggaagtctg
     1621 aagaatgttt gttgtctata ttcaaggagc ttacattgtt atcagaaaaa gagacactta
     1681 tattcgatga aactattact gcattgggtt tttctaatgt tttgaatatc aataaggtaa
     1741 tagacatgct tgtaactcat ttttctctaa aattaagctc acgattacct gttattgcta
     1801 tttacactat ttatcaagaa ttaattaaaa acgtaaaaag atacaatggt aagatattaa
     1861 aacctctgaa tgttcatact tccgctgata aacatggcta tggagatgtg gaaatatgga
     1921 acaaaaataa tacaccattt gaaatggttg aaataaaaca caatattgct attgatagaa
     1981 atttgatttt tgatgttgtg aaaaaatcaa aaaatacaac aataaaacgt tattatgtat
     2041 tgacaacttt taccgatagc ttcttatcaa aaaatgaaga gttgtatatt aataacttta
     2101 ttttaaaaat aaaacaagat actgatattg atgttattgc aaatggcatt ataccaagtt
     2161 tgaaatacta tctccgtttt attgatgatt ataaggcgtt tataaaatct tataccaaaa
     2221 atctaattgc tgatgcaaaa aattctacag aaataaaaga tttccattta acaacttggc
     2281 aagagatttt gaagaaaaat aatattgata aatgattaaa tccccattac gttatccagg
     2341 tggcaaaagt cgtgcagtaa aaactattac aaaaatctta attttaaagt caaatgcgat
     2401 aaaaatgcgt aagttttatt ttaaaaatct aaaattgt
//
LOCUS       DAOPUR010000227         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_45884_length_2426_cov_3.245888, whole genome shotgun sequence.
ACCESSION   DAOPUR010000227 DAOPUR010000000
VERSION     DAOPUR010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45884_length_2426_cov_3.245888"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKN90_04705"
     CDS             complement(<1..711)
                     /locus_tag="PKN90_04705"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNZ85612.1"
                     /translation="MKTKQEIIQNWLPRYTERPLKDFTDLILLTNFNNYVDIFAEWYG
                     VPVLAKNGMMRNASAEGITIINFGMGSPNAALIMDILPAIKPKAVLFLGKCGGLRDKN
                     RLGDYILPSAAIRGEGTSNDYFPPEIPALPAFSLQRAVSSNIRNMEKDYWTGTVYTTN
                     RRVWEHDEAFRAYLMKTRAMGIDMETATLFSAGFYNGIPIGALLLVSDMPLQKDGIKT
                     SDSDKHVTANFVEEHLLIG"
     gene            748..1197
                     /locus_tag="PKN90_04710"
     CDS             748..1197
                     /locus_tag="PKN90_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNZ85613.1"
                     /translation="MLEFELPRYTFRVKKQAEKTYILDAYRAKYVRATPEELVRQQMC
                     AYLQNERAYPKTMIFNECVLRINTMNKRCDTVIYSTAHTPIMLIEYKSPDIQISQAVF
                     DQVMLYNRVLQVPYILISNGKQHFCCKLDTDLQQYIFLNEIPAYNNL"
     gene            complement(1253..>2426)
                     /locus_tag="PKN90_04715"
     CDS             complement(1253..>2426)
                     /locus_tag="PKN90_04715"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNZ85614.1"
                     /translation="ITDDTRIRAAMPTLKKILADGGSVIIASHMGRPKKNPDAKYSLQ
                     YILKHVEKLLGVPVKFAADCAKASDMAASLKNGEALLLENLRFYAEEEGKARGLAEDA
                     TDEEKAIAKKAIKESQKEFTKTLASYADCYVNDAFGTAHRAHASTALIAKHFDENNKM
                     FGYLMEKEVLAVQKILSDIERPFTAIMGGSKVSSKIEIIENLLGKVDNLILAGGMTYT
                     FSKAMGGNIGISICESDKLDLALELIEKAKKNNVNLVLAVDAKIGDDFSNDCNTQFVS
                     VKEIPDGWEGLDIGPETEKIFAEVIKNSKTILWNGPTGVFEFENFTSGSKAVANAIVE
                     ATKKGAFSLVGGGDSVACINKFGLADQVSYVSTGGGALLEAIEGKVLPGIEAIRGF"
BASE COUNT          740 a          488 c          419 g          779 t
ORIGIN      
        1 tccaataagc aggtgttctt ctacaaaatt ggctgttaca tgtttgtcgc tatcgctcgt
       61 ttttataccg tctttttgta aaggcatatc agacaccaac aataaggctc caataggaat
      121 tccattgtaa aaacccgcag aaaatagggt agccgtttcc atatcaatac ccatggctct
      181 tgttttcatt aagtaagctc taaatgcttc gtcgtgttcc caaacgcgtc tattagtcgt
      241 atacacagtt cctgtccaat agtctttttc catgtttcta atatttgacg aaacagcccg
      301 ttgtaaacta aatgcaggta atgcaggtat ttcgggagga aaatagtcat tagacgttcc
      361 ttcgcctcgt atagctgcac taggtaaaat atagtcgcca agccgattct tgtcacgcaa
      421 tccaccgcat tttccaagaa atagaacagc ttttggtttt attgcaggta gaatatccat
      481 tatcagagct gcatttgggc tgcccattcc aaaattaatt attgtaatac cttcggcaga
      541 agcattgcgc atcataccat ttttggcaag cacgggtacg ccataccatt cagcaaaaat
      601 atctacataa ttattgaaat ttgtcaacaa aataagatct gtaaagtctt ttaatggacg
      661 ttcagtatat cgtggtagcc aattttgtat gatttcttgt tttgttttca tacctttgcg
      721 gtgttaatta cagcaaaagt actaaaaatg ttggaatttg aattacctcg ttacactttt
      781 cgtgtaaaaa agcaggcgga aaaaacatat attttggatg cttatcgtgc taagtatgtg
      841 cgtgccacgc ccgaagagct tgtgcgtcaa cagatgtgtg cgtatttaca aaatgaacga
      901 gcttatccta aaacaatgat ttttaacgaa tgtgtattac gtataaacac aatgaataag
      961 cggtgcgata cagtaattta ttccacagca cacacgccaa ttatgctaat agaatacaaa
     1021 tcacctgaca tacagataag ccaagcggta tttgatcaag tgatgctata caatcgtgtg
     1081 ttgcaagttc catacattct tataagcaat gggaaacaac atttttgttg taaactagat
     1141 actgatttgc agcaatacat ttttttgaac gagatacctg cctacaataa tttgtaaaaa
     1201 aaatgctacc cgtgtggata gcatttttat gataaaagta gggaatctta agttagaaac
     1261 cacgaatagc ttcaatacca ggaagaactt ttccttcgat agcttcaagt aacgcaccac
     1321 cacctgtaga tacatagctt acttggtcag ccaatccaaa tttattgata caagcaacag
     1381 agtcgccacc accaacaagt gagaaggcac ctttcttagt agcctcaaca atagcgttcg
     1441 ctacagcttt agagccactt gtaaagtttt cgaactcaaa tacacctgtt ggaccattcc
     1501 ataagattgt tttagagttt ttaattactt ctgcaaatat tttttcagtt tctggaccaa
     1561 tgtcaagacc ttcccatcca tcaggaattt ctttaacaga cacaaattgt gtgttacagt
     1621 cgttagaaaa atcgtcacca attttagcat ctacagccaa taccaagttt acattatttt
     1681 tctttgcttt ttctattaac tcaagagcta agtctaattt gtctgattcg cagatagaaa
     1741 taccgatatt accacccatt gctttgctaa aggtataagt cattccacca gcaagaatta
     1801 aattatctac tttacccaac aggttttcaa taatttcaat tttagaagat actttagaac
     1861 cacccataat agcggtgaaa ggacgttcga tatctgatag tattttttga acagccaata
     1921 cttctttttc catcaaataa ccaaacattt tgttgttttc gtcaaagtgt tttgcaatca
     1981 acgcagttga agcgtgagca cggtgagcag taccaaaagc atcgtttaca taacaatcag
     2041 cataagatgc caatgttttg gtaaattctt tttgactttc tttaattgct tttttagcta
     2101 tagctttttc ttcgtcagta gcatcttcgg ctaatccacg agctttgcct tcttcttcgg
     2161 cataaaaacg aagattttcg agtagtaacg cttctccgtt ttttagcgaa gcagccatat
     2221 cgctagcttt tgcacaatcg gcagcaaatt ttacaggtac acctagtaat ttttctacgt
     2281 gtttaagaat gtattgtaga gaatactttg catctggatt tttctttggt ctgcccatgt
     2341 gcgatgcaat aataacgcta ccaccatcgg caaggatttt tttcaacgta ggcatagccg
     2401 cacgaatacg ggtatcgtcg gtaatg
//
LOCUS       DAOPUR010000228         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_46263_length_2413_cov_2.279050, whole genome shotgun sequence.
ACCESSION   DAOPUR010000228 DAOPUR010000000
VERSION     DAOPUR010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46263_length_2413_cov_2.279050"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKN90_04720"
     CDS             <1..218
                     /locus_tag="PKN90_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85615.1"
                     /translation="LKKGEGYYQNTMLLLNSFNVGITDYFSIGGGIEFLSTFITLSEV
                     TLTPLSILHPKLVFPLPIIFMLVEECL"
     gene            209..580
                     /locus_tag="PKN90_04725"
     CDS             209..580
                     /locus_tag="PKN90_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85616.1"
                     /translation="MLIANIPQFGETTNSGLGVLYGLGTYGNLDHNVTATIGWGSVDG
                     DAFNKPLITVSGMTRVAKKTALVTENWLIPTDSSYEPIFSYGVRFFGENIAVDLAFIN
                     NWDIAQSLLIGVPYVNFVVKF"
     gene            736..1314
                     /locus_tag="PKN90_04730"
     CDS             736..1314
                     /locus_tag="PKN90_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85617.1"
                     /translation="MQGKLQELTEKIYQEGVQKAQKEVDILLQAATQKAHNIEQDAQK
                     KAKTIIAEAEKKAELLDKHVQSELKMSVSQATSALKQDLTTLLTAKAIEAPVKELFEN
                     KEFLQTLVQTVVSSWIAKGTYDLQVILPEKDKKELDAAFKKQLAKELSKGVELIFSDT
                     VKNGFKVVQSKEGYQLSFTDQDFILFQSLSTP"
     gene            1348..2211
                     /locus_tag="PKN90_04735"
     CDS             1348..2211
                     /locus_tag="PKN90_04735"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNZ85618.1"
                     /translation="MSKNYYALVAGLPDIMAQDKKLLYTSVQMRDIISEEVSEEDMSL
                     VQLLFLPFDHLNIVNALFKKQVSWDERGNLSSEIVEQLKDKKNIESVDLENVPSYIRD
                     FVERYYVEEQEINFYQAEFLLTEAYFEYLTTHTNSFVAALGYYYTSLNNLKVAYNGRK
                     HDVSYDKQLIGENELTHALRKSRTRDFGASTLVDNLESLLQIFEIENTIERELKIDNN
                     TWNFVEENTFFNYFSIERVMAFIVKLLIVERWLLLDEEKGKQMFNQLVTELQDGFQFS
                     HEYTLAYGKKK"
     gene            2195..>2413
                     /locus_tag="PKN90_04740"
     CDS             2195..>2413
                     /locus_tag="PKN90_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNZ85619.1"
                     /translation="MERKNKTTGKVVGIVANLVIVETNGPVAQNEICFIHLGQENLMA
                     EVIKIVGIKAYVQVFESTRGLKVGTSVTF"
BASE COUNT          864 a          363 c          452 g          734 t
ORIGIN      
        1 accttaaaaa aggcgaaggt tattaccaaa acaccatgct actacttaat tcattcaatg
       61 ttggtattac agattatttt tcgataggtg gaggaataga atttttatct acatttatta
      121 ctttaagtga ggtaacttta accccattat ctattttaca cccaaagttg gtttttccgt
      181 tgccgataat attcatgttg gtggaggagt gcttatagca aatattccgc aatttggaga
      241 aacaacgaat tcaggcttag gtgtattata tgggctcggt acgtatggca atcttgacca
      301 taacgtaact gctaccattg gttggggatc tgttgatgga gatgctttta acaaaccatt
      361 gataacagta tctgggatga ctcgtgttgc taaaaaaaca gccttggtaa ctgaaaattg
      421 gcttattcct acagactcat cctacgaacc tatattttcg tatggggttc gtttttttgg
      481 agagaatatt gctgtcgatt tggcttttat aaataattgg gatattgcac aatccttact
      541 aataggagtt ccatatgtga attttgttgt gaaattttga tttgttagcg ttgcaaaatg
      601 tattgaaaaa gtgaaaatta tcctaaatat aaagatttta ggataatttt ttacgtttat
      661 ttttttttca aaaataaaaa taagtatctt cgtggtgttc aataaacgaa tattttattt
      721 caaaactaat cgaatatgca aggaaaactt caggaattaa ctgaaaaaat ctaccaagag
      781 ggagtgcaaa aagcacaaaa agaagtagat attttattac aagcagccac ccaaaaagcg
      841 cacaatatag aacaagacgc acagaaaaaa gccaaaacta tcattgccga agctgaaaaa
      901 aaagctgaac tgctagataa acatgtgcaa tcggaattga aaatgtctgt ttctcaagcc
      961 acttcagccc ttaaacaaga tttgacaaca ctgttaacag ctaaagctat tgaggctcca
     1021 gttaaagaat tgtttgagaa caaagaattt ttgcaaactc ttgttcaaac tgttgtaagt
     1081 agttggatag caaaaggaac atacgatttg caagttattc ttccagaaaa agataaaaaa
     1141 gaattggatg ctgctttcaa aaaacagcta gcaaaagaat tgagcaaggg agtagaattg
     1201 attttttctg ataccgtaaa aaatggattc aaggtggttc aatcgaagga aggttatcaa
     1261 ttaagtttta ccgatcaaga ttttatacta tttcaaagct tatctacgcc ctaaaactac
     1321 tgaattactc ttcgaaaaag aataagaatg tcaaaaaatt actatgcatt agtagccgga
     1381 ttacccgata tcatggctca ggacaaaaaa ttactttaca cttctgtaca gatgcgagat
     1441 atcatcagcg aagaagtgag cgaagaagat atgtcgttgg tgcaattatt gtttctgcca
     1501 ttcgatcatc ttaatatagt aaatgcatta ttcaaaaaac aagtttcttg ggacgaaaga
     1561 ggaaatcttt cctctgaaat cgtggaacaa ttaaaagata aaaaaaacat tgaaagcgtt
     1621 gatttagaaa atgtaccaag ctacatccga gattttgttg aaagatatta tgtagaagag
     1681 caagaaatca atttttatca ggctgaattt ttacttactg aggcttattt tgagtattta
     1741 acaacccata caaattcctt tgttgctgca ttaggctatt actatacatc actaaataac
     1801 cttaaagtag cttataatgg gcgaaaacat gatgtatcgt atgacaagca attaatcgga
     1861 gaaaatgagc taacacatgc cctgagaaaa agtcgcacac gcgattttgg agcatcaact
     1921 ttggtggata acttggaaag cctgttgcaa atttttgaga tagaaaatac aattgaaaga
     1981 gagctaaaga ttgacaacaa tacgtggaat tttgttgaag aaaatacttt cttcaattac
     2041 ttctccattg agcgtgttat ggcttttatt gttaaactgc tcattgtaga aagatggctg
     2101 ctcttagacg aggaaaaagg aaaacaaatg tttaatcaat tagtaacaga actgcaagat
     2161 ggatttcagt tctcgcatga atatacacta gcatatggaa agaaaaaata aaactactgg
     2221 caaagttgtt ggtatagtag ccaatttggt gattgtagaa accaatggac ccgttgctca
     2281 aaatgaaata tgctttattc atcttggtca ggaaaattta atggctgagg taattaaaat
     2341 tgttggaatc aaagcatacg ttcaagtttt tgaaagcaca cgtggactaa aagtcggtac
     2401 gagtgtaaca ttc
//
LOCUS       DAOPUR010000229         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_46357_length_2410_cov_3.143100, whole genome shotgun sequence.
ACCESSION   DAOPUR010000229 DAOPUR010000000
VERSION     DAOPUR010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46357_length_2410_cov_3.143100"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PKN90_04745"
     CDS             complement(<1..522)
                     /locus_tag="PKN90_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HNZ85620.1"
                     /translation="MMKRNLLVTILLGLIVCNLQAANSAYVTQIIDYRPAPGQHINRL
                     FPPADKSDSHENAVAWAATQLVGKTNGLVGLGSFGGYIIVGFDHSIVNVAEEYDFKVY
                     GNAFANSAEPGIVMVCQDLNGNGVPDNDEPWYELAGSDYQLSSTIKNYEITYTRPNPD
                     LQKSHIQWTDNQGG"
     gene            complement(536..1696)
                     /locus_tag="PKN90_04750"
     CDS             complement(536..1696)
                     /locus_tag="PKN90_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNZ85621.1"
                     /translation="MNKKNYSIPIAILSVLIVAFFTACRTDEPVLLPSKTPVTDEATN
                     SEVVGFYLLNEGNMGSNKATLDYFDFTTGIYTKNMYAATNPQVVSELGDVGNDLQIYG
                     NKLYAVLNTSGFVEVMDATTAKHIGMIEIPNCRYITFYNGKAYVSSYAGPVELSNNTQ
                     VGYVAEIDTASLQITRTVHVGYQPEEMAIVNGTLYVANSGGYMYPEYDNTVSVICLQT
                     FTETQKVEVGINLHRLKSDNKGNLYISSRGNYTDIASCLYILNTNTLQVDKKVDIPVS
                     NLCIVGDSAYVISSEFNFLSGNTNIEYALINTSTQNIEANNFITDGTETAIKMPYGIA
                     VHPITRDIYITDAKSYIIPGTVYCYSKTGKRKWYVTTGDIPAHIAFLTKQNN"
     gene            complement(1697..>2410)
                     /locus_tag="PKN90_04755"
     CDS             complement(1697..>2410)
                     /locus_tag="PKN90_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ85622.1"
                     /translation="PFNLHGFYKRIFRVPTFNDLYYTFVGNASLQPEYVSQYAIGAKY
                     ASAQKNTSFTPSFDIQIDGYYNRVEDKIIAVPTANPFRWQMTNLGLVEIIGTDISVQT
                     SMNFSKDVLGLLKMNYTYQLAEDKTDKTEEYYGHQIPYIPWHSGTAVVAVAYKKWSFN
                     YSFIYTGERYNASANIPENYVQPWYTHDASISKDFALFGYQWTALVEINNLLNQQYDV
                     VLNYPMPGTNFKCSLKIRI"
BASE COUNT          707 a          450 c          450 g          803 t
ORIGIN      
        1 tccaccttga ttatcagtcc attggatatg cgatttttgt aaatcaggat tgggtcgtgt
       61 gtaggtaatc tcatagtttt taatagtgct tgacagttga taatcagaac cagccaattc
      121 gtaccaaggt tcatcgttgt cgggtactcc attgccattc aagtcttgac aaaccatcac
      181 aatgcctggc tctgcactat tagcaaaagc attgccatat actttaaagt catattcttc
      241 ggctacattc acaatagaat ggtcgaatcc aacaataata tatccgccaa aagatcccag
      301 tccaaccaaa ccattggttt tacctaccaa ttgagtagct gcccaagcca ccgcattttc
      361 gtgagaatca ctcttatcag caggaggaaa taaacgattg atatgttgcc caggtgctgg
      421 acgataatca ataatttggg ttacgtaagc agaatttgct gcttgtaagt tacacacaat
      481 caatcctaaa agaatcgtta caagtaaatt tcttttcatc attaagtgag ttaagttagt
      541 tattttgttt ggttaagaat gcaatatgag caggaatatc acccgtggta acgtaccatt
      601 ttcgtttgcc agttttgctg tagcaatata cagtaccggg aataatgtaa cttttcgcat
      661 cagtaatata gatatctcgc gtaatagggt gtactgctat gccataaggc attttgatgg
      721 ctgtttctgt tccatctgtg ataaaattat ttgcctcaat gttttgtgta cttgtgttga
      781 ttaaggcata ctcaatgttg gtattgcctg acaaaaaatt aaattctgaa ctgataacat
      841 aagccgaatc gcccacaata cataggtttg aaacaggaat atcaaccttt ttatctacct
      901 gcaaggtgtt ggtgttaagg atatacaagc acgaagcaat gtcggtgtag ttgcctctac
      961 tactgatata taggtttcct ttattgtctg attttaatcg atgaagatta atgcctactt
     1021 caactttttg tgtttcggta aaagtttgta ggcaaattac agaaaccgta ttatcatact
     1081 ctggatacat atatccgcca gaattagcaa catacagcgt accattaact atagccattt
     1141 cttcgggttg gtaaccaaca tgtactgttc gggttatttg caaacttgct gtatcaattt
     1201 cagctacata acctacttgc gtattgttac ttaactcaac aggtccagca tacgaactta
     1261 cataagcttt tccattataa aatgtgatat aacggcaatt gggtatttct atcatgccta
     1321 tatgtttggc agtagtggca tccattactt ctacaaatcc agaggtgttg agaacagcgt
     1381 ataatttgtt tccataaatt tgcaaatcat tgccaacatc acccaattct gatactacct
     1441 gagggttggt agccgcatac atatttttag tatatatgcc tgttgtaaag tcgaaataat
     1501 cgagcgtagc tttgttgctc cccatattac cttcgtttaa caaataaaaa cccactacct
     1561 cgctattagt tgcttcgtcg gttacaggcg ttttacttgg caaaagtaca ggctcatctg
     1621 tgcgacaagc tgtaaaaaat gccactatta gcacagagag aatagctatt ggtatggaat
     1681 aatttttttt gttcatttat atacgtattt ttaagctaca tttaaaattc gttcccggca
     1741 ttgggtagtt tagtaccaca tcatattgtt ggtttagtag gttattgatc tccactaaag
     1801 cagtccattg ataaccaaat aatgcaaaat ctttcgaaat gcttgcatca tgtgtgtacc
     1861 aaggttgtac atagttttcg ggtatattgg cactggcatt gtatcgttcg ccagtgtaga
     1921 taaagctata attgaacgac cattttttgt atgcaacagc tactactgcg gtgccgctat
     1981 gccaagggat ataaggtatt tgatgaccat aatactcttc tgttttatcc gttttgtctt
     2041 ctgctaattg gtaggtatag ttcatcttga gcaagcctaa cacatctttt gaaaaattca
     2101 tactcgtttg taccgaaatg tctgtaccaa ttatctctac caatcctaaa tttgtcattt
     2161 gccaacggaa aggattggct gttggtacgg caataatttt atcttctaca cggttgtaat
     2221 agccatcaat ttgaatatca aaagaggggg taaaacttgt gtttttttgt gcagaggcat
     2281 attttgctcc aatggcatat tggcttacat attcaggttg taaagacgca tttcctacaa
     2341 aggtatagta caaatcgttg aatgtgggta cacgaaagat gcgtttataa aatccatgta
     2401 ggttaaatgg
//
LOCUS       DAOPUR010000230         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_46776_length_2395_cov_4.485470, whole genome shotgun sequence.
ACCESSION   DAOPUR010000230 DAOPUR010000000
VERSION     DAOPUR010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46776_length_2395_cov_4.485470"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1734
                     /locus_tag="PKN90_04760"
     CDS             124..1734
                     /locus_tag="PKN90_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85623.1"
                     /translation="MIKLDNKFTSDFKDHIKFSYSFFDRIIVRGYIIGMFRPANVIAL
                     LRNLGFSKHTNGVFKLLSDQLNNHIKKLAAKLEVPILWRENHGGKEMEMQSYVEENYF
                     NPNKLGVICIIKSLEFMNCYWNKQITTKSNTFFTKMFWLKKQVTQHYIYINDSVLGFC
                     CLKISSGLPFHCQFYCNGHWYLRRQLDKKGIGYKMHENAFLSIDDESFANTLIKDFSG
                     SIIDSRIKYWIERWFRFDKGERSTCSSLLTHNWYTSQCEVCSNIIFKNKDYFNRVYDK
                     ILEKHHNLATPDSLSRLFELKQERKQSKSTNKVYHQKACVKYWIQGNSIKMYNKGGYL
                     LRVETTINNPRLPGAKLQKPIFGIKGYYWYGFGCNNRFFNAISKIDATLLGDNENEFT
                     TAVHTNKGKRVAAPDLRNPKQLAILAVLSNPRFTTEWFRTKDLMRYLSGYFAKTAEIR
                     YQMEKLRVRGLIEKRQSANYYRVTEKGYSWLNVAYSYNRYFLTPLLSSNLQIELYIKN
                     KGLDKFEQAQKQIKAGLNAIYQELNISA"
     gene            1792..2262
                     /locus_tag="PKN90_04765"
     CDS             1792..2262
                     /locus_tag="PKN90_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNZ85624.1"
                     /translation="MLNEISSPDKIFAYGKSNGGFIMGNMANYYPEYFNGIILDVPYF
                     DVLTDFSNSDDYFYLGSIENPDEFNNLKKIDPYQNIKPQDYPNLLFFGALQDINVEPS
                     SYIKAVAKLRQTKTDNNVLLLRVLMNSGHGVDLGKIQQKQALIYSFMLNCLPKE"
     gene            2266..>2395
                     /locus_tag="PKN90_04770"
     CDS             2266..>2395
                     /locus_tag="PKN90_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HNZ85625.1"
                     /translation="MSKFPHYQQLDAMDCGATCLRMIAKYYGRLYTAQTLRQRSSIT"
BASE COUNT          870 a          377 c          411 g          737 t
ORIGIN      
        1 ataataccgc ctgtggcggt cgaatttttt tttttgaaat attcactata tgatagcgac
       61 ctaaaggtct tggggttggg gtaatcagtt aaactaaaat ttttattttt gcattaaaaa
      121 agcatgataa aattagacaa taagtttaca tcggatttca aagaccatat aaaattcagt
      181 tacagttttt tcgatcgcat aatagtacgt ggttatatta ttggaatgtt ccgtcctgct
      241 aatgttatag ctttattgcg taatttaggt ttttcgaaac acacaaatgg agtttttaaa
      301 cttttatcag accaattaaa taaccacata aaaaaattgg ctgctaaact tgaagtccca
      361 atattatgga gagaaaatca tggtggtaaa gaaatggaaa tgcagtctta tgtagaggaa
      421 aattatttca atcctaataa gttaggcgtg atttgcataa taaagtcctt agaatttatg
      481 aactgttact ggaataagca aatcacaact aagagcaata catttttcac taagatgttt
      541 tggctaaaaa aacaagttac tcaacattac atctacataa acgattctgt attaggattt
      601 tgttgtctaa aaatatcatc aggcttaccg tttcattgtc agttttattg taacgggcat
      661 tggtatttac gtcgtcagct agataaaaaa gggattggtt acaaaatgca tgaaaatgct
      721 tttctttcaa tagatgatga atcttttgca aacacattga ttaaagattt tagtggcagt
      781 attatagata gtcgaataaa atactggata gaaagatggt ttcgtttcga taaaggggag
      841 cgttcaactt gctcatcatt actaactcac aattggtaca ctagtcaatg tgaggtttgt
      901 agtaatataa ttttcaaaaa caaagactat ttcaacaggg tttacgacaa aatactagaa
      961 aaacatcaca atttagcaac accagacagt ttgagtaggc tgtttgaatt aaaacaagag
     1021 agaaagcaaa gtaagagtac aaataaagtt tatcatcaaa aagcctgtgt caaatactgg
     1081 atacaaggca attcaatcaa aatgtacaat aaaggcggtt atttgctccg agttgagacc
     1141 acaataaata accccagatt gcctggggct aaactacaaa agccaatctt tggaataaag
     1201 ggctactact ggtacggctt tggatgcaat aaccgcttct ttaacgctat ttcaaaaata
     1261 gatgcaacat tacttggaga taatgaaaat gagtttacca ctgcggtaca cacaaacaaa
     1321 ggtaaacggg tagcagctcc cgacctaaga aatccaaaac aacttgcaat tttagcggta
     1381 ctatcaaacc ctcgttttac aacagaatgg tttcgaacca aagacttaat gcgatatttg
     1441 tcaggctatt tcgctaaaac tgcagaaatc cgttatcaaa tggaaaaact aagggtaaga
     1501 ggactgatag aaaaacgtca aagtgctaat tactaccgag ttacagaaaa aggctattca
     1561 tggttaaatg ttgcttattc ttataatcgc tatttcttaa ctcccttatt atcaagtaat
     1621 ttacaaattg aactatacat caaaaacaag ggtcttgata aatttgagca agcacaaaaa
     1681 caaattaaag caggcttaaa cgcaatatat caagaactta acatctctgc gtaaaaaaca
     1741 aaaacgagaa tttttttcac gtttttcaat tactaaaaca tagaatattt aattctaaac
     1801 gaaataagta gcccagataa gatttttgct tatggtaaaa gcaatggtgg ttttattatg
     1861 ggcaatatgg cgaattatta tccagagtat tttaacggaa taatattaga cgttccatat
     1921 tttgacgttt taactgattt ttcaaattcc gatgactatt tttatcttgg atcaatagaa
     1981 aatccagatg aatttaataa tcttaaaaaa atagacccat atcagaatat aaagccgcaa
     2041 gattatccaa atcttttgtt ttttggtgct ttgcaagata ttaatgtgga accttcttca
     2101 tatataaaag ctgttgccaa gctacgtcaa acaaaaacag ataataatgt tttactgtta
     2161 agagtattaa tgaattcggg acatggtgtg gatttgggca aaatacaaca gaaacaagct
     2221 ttgatctatt cttttatgtt gaattgttta cctaaggaat agacaatgag taagttcccc
     2281 cactaccaac aactcgacgc catggattgc ggagcaacct gcttacgcat gattgctaag
     2341 tattatgggc gattatatac ggcacaaact cttaggcaac gttcaagtat tactc
//
LOCUS       DAOPUR010000231         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_46840_length_2393_cov_3.288281, whole genome shotgun sequence.
ACCESSION   DAOPUR010000231 DAOPUR010000000
VERSION     DAOPUR010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46840_length_2393_cov_3.288281"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1096)
                     /locus_tag="PKN90_04775"
     CDS             complement(77..1096)
                     /locus_tag="PKN90_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNZ85626.1"
                     /translation="MGLFSFTQEIAIDLGTANTIIIQNGKIVLDEPSIVALDKRTDKL
                     IAIGEKARQMQGKEHESIRTVRPLRDGVIADFYAAEIMIRGMIKMISNKNSFFAPSLK
                     MVVCIPSGSTEVEIRAVRDSSEHAGGREVYMIYEPMAAAMGIGLDVEAPEGNMIVDIG
                     GGSTEIAVISLGGLVCNRSIRIAGDDLTSDIVDYMGRQHNIRIGERTAELIKINVGSA
                     LTDLEDAPEDYIVRGPNRLSALPMEVPVSYQEIAHCLDKSISKIEIAVLGALEQTPPE
                     LYADIVLNGIYLAGGGALLKGLDKRLQDKINIPFHIAEDPLRAVARGTGIALKNVDNY
                     SFLLR"
     gene            complement(1118..>2393)
                     /gene="purH"
                     /locus_tag="PKN90_04780"
     CDS             complement(1118..>2393)
                     /gene="purH"
                     /locus_tag="PKN90_04780"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNZ85627.1"
                     /translation="KLEGDMNQLAEYDIPEIDLVIVDLYPFEATVASGASEQEIIEKI
                     DIGGISLIRAAAKNFADVIIVASQAQYAPLYDLLEENGAESSIEQRKYFAKEAFAVSS
                     YYDTAIFNYFDGYENSAFRHAENTMKALRYGENPHQRGFFYGKFNDMFEQLQGKEISY
                     NNLLDIDAAVSLINDFDEITFAILKHNNACGIASRSNLLDAWKDALAGDPVSAFGGVL
                     ITNAIIDQTTAEEINKLFFEVIIAPDYDMEALEILSQKKNRIILIQKETKSKTTQFRS
                     LLNGVLVQDKDLSIETEKDFKVVTQIEPSSSETEDLIFANKIVKHTKSNAIVLAKNKQ
                     LCASGVGQTSRVDALRQAIEKANSFEMSLKGAVMASDAFFPFPDCVEIASQAGITSVI
                     QPGGSIKDDLSIEFCDNNNMSMVTTGIRHFKH"
BASE COUNT          691 a          454 c          421 g          827 t
ORIGIN      
        1 gtataaaaaa caaattgtca gcaaaaaaga aatatttctg ccgacaatag tacattctct
       61 catgtatagt gaggtattac cttaataaga atgaataatt atcaacattt ttaagagcaa
      121 ttcctgttcc tcttgctact gcacgtaagg gatcttctgc aatatgaaat ggaatattga
      181 ttttatcttg caaacgttta tcaagtcctt ttagtaaagc tccacctcct gctaagtaga
      241 taccattgag aacgatatca gcatacaatt cgggaggagt ttgttctaat gcccctaaca
      301 ctgcaatttc aatttttgat atagatttgt ctaaacaatg agctatttct tggtatgata
      361 caggaacttc cattggtaga gctgatagcc tatttggtcc gcgtacaatg taatcttctg
      421 gtgcatcttc aagatctgtt agtgccgatc cgacgtttat tttaatgagt tcagctgtcc
      481 tttctccaat tcttatattg tgttgtcttc ccatataatc tacaatatcc gaggtaagat
      541 cgtctccagc aatacgaata gaacggttgc atactaatcc tcctaaagat attacggcaa
      601 tttcggtgct accgccacct atatctacaa tcatattgcc ttccggtgct tctacatcaa
      661 gacctatacc cattgctgct gccataggtt cgtatatcat atatacttct ctacccccag
      721 catgttcaga agagtcacgt acggcacgta tttcaacttc ggtgcttccc gatggaatac
      781 aaacaaccat tttaagagaa ggagcaaaaa atgaattttt gttacttatc atttttatca
      841 tgcctctaat cataatttcg gctgcataaa aatcggcaat aacaccatcg cgtaatggac
      901 gaacagtacg tatgctttcg tgctctttac cttgcatttg acgtgccttt tctccaatgg
      961 caatcaattt gtcggtacgt ttatcaaggg caacaataga tggttcgtct aatacaatct
     1021 tcccattttg aatgatgatg gtatttgctg taccaaggtc aatggctatt tcttgagtga
     1081 atgaaaataa tcccatgtgt ctatgtttgt taaattttta atgtttaaaa tgtctgattc
     1141 ctgtggttac catcgacata ttattgttgt cgcaaaactc aatagataaa tcgtctttga
     1201 ttgaacctcc tggttgaata acagatgtaa ttcctgcttg tgatgctatt tctacacagt
     1261 cggggaatgg gaaaaatgca tctgaagcca ttactgcacc tttcaaactc atttcaaatg
     1321 aatttgcttt ttctatggct tgacgcagag catctacacg tgatgtttgt cctacgccac
     1381 tagcacataa ttgtttgttt ttagctaata caatggcatt tgatttcgtg tgttttacaa
     1441 ttttatttgc aaaaataagg tcttcagttt ctgatgatga cggttcaatt tgtgtaacaa
     1501 ctttaaaatc tttttcggtt tcaatgctca aatctttatc ttgtactaag acgccattga
     1561 gtaatgagcg aaattgagta gttttgcttt tagtttcttt ttgaattaaa atgatacgat
     1621 ttttcttttg tgataatatt tctaatgctt ccatgtcata gtcgggagca ataattactt
     1681 cgaagaaaag tttattgatt tcttcagccg tagtttggtc aatgattgca ttggtaatta
     1741 acacaccccc aaatgctgac acagggtcac cggctaaggc atctttccat gcgtcgagta
     1801 aattgctacg agatgcaata ccacaggcat tattgtgttt taagatggca aatgtaattt
     1861 cgtcaaaatc attaattaaa ctaacggcag catcaatatc taataaattg ttgtacgata
     1921 tttctttccc ttgcaattgc tcaaacatat cgttgaattt tccgtagaaa aatccacgtt
     1981 gatgaggatt ttcgccgtat cgtaaagctt tcatggtatt ttctgcatga cggaaagcac
     2041 tattttcgta tccatcaaaa tagttgaaga tagcagtatc gtagtatgaa gatacagcaa
     2101 aggcttcttt ggcaaagtat ttgcgttgtt caatagaact ttcagctcca ttttcttcta
     2161 gcaaatcgta tagtggagcg tattgagctt gcgatgcaac aataattaca tcggcaaagt
     2221 tttttgcggc tgcacggata agggatatgc caccaatatc aattttttca ataatttctt
     2281 gttcgctagc tccagatgct acagtagctt cgaatgggta taagtcaacg attactaaat
     2341 caatttctgg aatgtcgtat tcagctaatt ggttcatatc gccttctaat ttt
//
LOCUS       DAOPUR010000232         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_47087_length_2383_cov_3.250430, whole genome shotgun sequence.
ACCESSION   DAOPUR010000232 DAOPUR010000000
VERSION     DAOPUR010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47087_length_2383_cov_3.250430"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="PKN90_04785"
     CDS             <1..153
                     /locus_tag="PKN90_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNZ85628.1"
                     /translation="SIISIGFSIFMFMLELLVAFIQAYVFTTLSAIFIGLSRVEPHHH
                     PKKEIN"
     gene            184..438
                     /gene="atpE"
                     /locus_tag="PKN90_04790"
     CDS             184..438
                     /gene="atpE"
                     /locus_tag="PKN90_04790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNZ85629.1"
                     /translation="MLLSIILQAVASASLAKLGASLGAALAVVGAGLGIGKIGSSAME
                     AIARQPEAASDIRSSMIVSAALIEGATLFAIIVCALAILI"
     gene            483..983
                     /gene="atpF"
                     /locus_tag="PKN90_04795"
     CDS             483..983
                     /gene="atpF"
                     /locus_tag="PKN90_04795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNZ85630.1"
                     /translation="MDLFLPESGLVIWMTIAFGIVFFILSKFGFPIILKSINEREKYI
                     IKSLDAANEAQQKLLEIEDKGKEIIANAEQQQLLIVKESQELKKKLVEEAKMQAQKEA
                     DNIIANARIQIEKEKETALSDIRNEVLQVSLSIAEKVLEKELENKKAHTQYVEKLLDN
                     MSMSQS"
     gene            992..1528
                     /gene="atpH"
                     /locus_tag="PKN90_04800"
     CDS             992..1528
                     /gene="atpH"
                     /locus_tag="PKN90_04800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNZ85631.1"
                     /translation="MNEGKISVRYARAILNLAKNKGIEDAVYKEMFVLSDNLTHYAKD
                     FKTAFAHPDITEENKKRLFKLAVGNTISSFTSQSIDFIVEKKRSSFMLRIALMYQTLY
                     QIEHNIIISNVISAKELDKKTLDKIKAFASKTFDASVELVSTIDPDLIGGFILDIANQ
                     RLDASIKGKLNNIRKIVY"
     gene            1540..>2383
                     /locus_tag="PKN90_04805"
     CDS             1540..>2383
                     /locus_tag="PKN90_04805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861786.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNZ85632.1"
                     /translation="MMASIKASEVSNLLQMELEGFSDKLKFEETGKVLQISDGVARVF
                     GLTKVQSNELVLFENGTKGVVLNLEEDNVGIVLLGSTYGIKEGDTVQRTGNIASILAG
                     EGLLGRVVNTLGEPIDGKGEIKGELFEMPLERKAPGVIFRQPVNEPLQTGIKAIDAMI
                     PIGRGQRELIIGDRQTGKTEIAIDTIINQRSNFLAGDPIYCIYVGIGQKGSTIAGIVK
                     TLEEYGAMDYTIVVSATASDPAAMQFYAPYAGVAIAEYFRDTGRPALIVYDDLMFFFL
                     PIRES"
BASE COUNT          855 a          354 c          460 g          714 t
ORIGIN      
        1 tctattattt caatcggatt ctccatattt atgtttatgc tcgaattact ggtggctttt
       61 atacaagcgt atgtatttac tactttgtca gctattttta taggtttatc tcgagtagaa
      121 cctcatcatc atcctaaaaa agaaataaat taaaataaat cattcactta aaacaaaaaa
      181 actatgttat tatcaattat tcttcaagca gtagcatcag cctctttggc aaaattagga
      241 gcaagtttag gtgcagcatt agctgtagtt ggagctggtt taggtatcgg taaaattggt
      301 agctcggcaa tggaagccat tgctcgtcaa ccagaagctg ctagtgatat tcgttcaagt
      361 atgattgtgt cagcagctct tattgaaggg gctactttat ttgcaattat cgtatgtgct
      421 ttggctattc ttatttaagt aaattgccac tataaatacc tttaaattta ataaatagtc
      481 ttatggattt atttcttccg gaatcaggat tggtaatttg gatgaccata gcctttggta
      541 tcgtcttttt tattttgtca aaatttgggt tccctattat tttgaaatct attaatgaac
      601 gagaaaagta cattatcaaa tcgcttgatg cggctaatga ggctcaacaa aaactcctag
      661 aaatagaaga caaagggaaa gaaattattg ccaatgctga acaacaacaa ctcttgatag
      721 ttaaagagtc tcaagagtta aaaaagaaat tagttgaaga ggctaaaatg caagcacaaa
      781 aggaagctga taacattata gcaaatgctc gcattcaaat tgaaaaagaa aaagaaacag
      841 ctttatctga tatacgtaat gaagtacttc aggtatcctt gtctattgca gaaaaggtac
      901 ttgaaaaaga actcgaaaat aaaaaagctc atacccagta tgtagaaaag ttattagata
      961 atatgtcaat gtctcaatca tagaattata catgaacgaa ggtaaaatat cagttagata
     1021 tgctagagca atccttaatt tagctaaaaa taaaggcatt gaggatgctg tgtacaaaga
     1081 aatgtttgta ctttcggaca acttaactca ttacgcaaaa gattttaaaa ccgcttttgc
     1141 ccatcctgac ataaccgaag aaaacaaaaa aagattgttt aaacttgctg ttggtaatac
     1201 catctcatca tttacatcac aatctattga ttttattgtg gaaaaaaaaa gaagctcatt
     1261 tatgctacgc attgcattaa tgtatcaaac tttatatcaa atagaacaca atattatcat
     1321 tagcaatgta ataagtgcta aagaattaga caaaaaaaca ttagataaaa taaaagcgtt
     1381 tgcaagtaaa acgtttgatg catctgtaga actagtcagt acaatagatc ccgacttaat
     1441 aggagggttt attttagata ttgccaacca acggctagat gcgagtatta aagggaaatt
     1501 aaacaatatt agaaaaatag tatactaaaa atatatccaa ttatggcaag cataaaagca
     1561 agtgaagtgt cgaatctgct acaaatggaa ctagaaggtt ttagtgataa actaaaattt
     1621 gaagagacag gcaaggtgtt acaaataagt gacggtgtag caagggtttt tggattaact
     1681 aaggtgcaat cgaatgagct tgtacttttt gaaaatggca caaagggagt agttttaaat
     1741 ctggaagaag acaatgtagg tattgtttta ttgggatcta cctatggaat taaggaagga
     1801 gatactgtac aaagaacagg aaatattgct tctatcttag ctggcgaagg actattaggg
     1861 cgtgtagtaa atacattggg cgaacctata gatggaaaag gcgaaatcaa aggcgaatta
     1921 tttgaaatgc cattggaaag gaaagctcca ggtgtaatat ttagacaacc cgtaaacgaa
     1981 ccactacaaa caggaattaa agccattgat gctatgattc cgattggacg tgggcagaga
     2041 gagttaatca ttggagacag acaaacgggt aaaacagaaa tagccattga tacgataatc
     2101 aatcaacgta gtaatttttt agcaggagat cctatttact gtatttatgt gggtatagga
     2161 caaaaagggt cgaccatagc tggcattgta aaaaccctcg aagagtatgg tgccatggat
     2221 tataccatag tggtatctgc aacagcctct gaccctgctg caatgcaatt ttatgctccc
     2281 tatgcaggag ttgccattgc tgaatatttt agagatacag gtcgtccagc tttaattgtt
     2341 tatgatgatt tgatgttctt ttttctgcca attcgtgaaa gtg
//
LOCUS       DAOPUR010000233         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_47156_length_2381_cov_2.649613, whole genome shotgun sequence.
ACCESSION   DAOPUR010000233 DAOPUR010000000
VERSION     DAOPUR010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47156_length_2381_cov_2.649613"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PKN90_04810"
     CDS             complement(<1..1211)
                     /locus_tag="PKN90_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNZ85633.1"
                     /translation="MSKQSKNQENGVKAPKKPSRFFANLISFFKNEKLHFILGMVLFA
                     FAAFSVLSLLSFLTLGENDYNLLGLSFEELAEQNKQFENFGGVTGAHLANIVINKWFG
                     VSSFLLVFFIISVAYKFLNPTTSYSLFKRFIFSAFFTIWISLAFGFLLPEISQLSFIQ
                     LGGNHGNEMAKILQLSMGYSGVILLLAVSLFMFLIIGFAATLPFFQKIANFNVSSLFR
                     KKKIEETVLENSEEIEAMENSPEENDSETEEFEVFEVEEENEEKEDISFEVIEPTNED
                     AELVEQATNEEEIDSEKLEEVTNENENGVKLVIEEGVKEEIYDKIAELGEYDPTLELP
                     HYKYPTFDLLQKYDSNNEINLEEQSANKNRIIQTLDNYGIKIKEIKATVGATVTLYEI
                     IPEAGVRISKIR"
     gene            complement(1324..2145)
                     /locus_tag="PKN90_04815"
     CDS             complement(1324..2145)
                     /locus_tag="PKN90_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85634.1"
                     /translation="MKLEEIACTRHFLLVSDADYFPINAYACALQFAQGYEKNLSFLV
                     LLTKKQLAHRDVIYETWNRQIETLQKKESIEIKVFFTESYFSELVPFTEQAETPMLFF
                     EISKNTQFNNPLSIFKQLKELRIPFVITKDSQTNPIDLSKIIVPIGFLTEEKEKAPYS
                     CNMGRFLHSEIILYQANDYGSKTTKNVESVLKLYNQFSLKVSIIKAKADSFGVEKEAT
                     MTTEKMQAGMVLVSTSRDYGLDDILFGPKELHCLKRATVPIMFINPRPDLYVLCW"
BASE COUNT          759 a          401 c          355 g          866 t
ORIGIN      
        1 cgaatttttg agatacgaac acctgcttct ggtatgattt cgtataaagt tacggtagca
       61 cctacagtag cttttatttc ttttattttt ataccataat tatcaagcgt ttgaatgatt
      121 ctatttttat tagccgattg ttcttcgaga tttatctcgt tgtttgagtc atatttttgc
      181 agtaaatcaa acgtaggata tttataatgt ggcaactcta gggtagggtc atattcgcct
      241 aattctgcta ttttatcgta tatttcttct tttacacctt cttcgattac tagttttaca
      301 ccgttttcat tttcgtttgt tacctcttct agcttttcag agtctatttc ttcttcatta
      361 gtggcttgtt ctacaagttc agcatcttca ttagtgggtt ctattacttc aaagcttata
      421 tcttcttttt cttcattctc ttcttcaacc tcaaatactt caaattcttc tgtttctgaa
      481 tcattttctt ctggagaatt ttccattgct tcaatctctt cactattttc tagtaccgtc
      541 tcctctattt ttttctttct aaaaagagaa ctaacgttaa agttggcaat tttttggaaa
      601 aacggtaaag tagcagcaaa accaataatt aaaaacataa ataaggatac tgcaagcaac
      661 aatatcacac cactatatcc catagatagc tgtaaaattt ttgccatttc attaccatga
      721 ttaccaccca gttgaataaa tgataattga gaaatttctg gcaatagaaa accaaaagcc
      781 aatgatatcc aaatggtaaa aaacgcacta aaaataaaac gtttgaacaa agaatatgaa
      841 gtggtaggat tcaaaaattt atacgctact gaaatgatga aaaagactaa tagaaaagac
      901 gaaaccccga accacttgtt tattactata ttagctaaat gagccccagt tactccacca
      961 aagttttcaa actgtttatt ctgttcggct aattcttcaa aacttaatcc gagtaaatta
     1021 tagtcatttt ctccgagagt taaaaatgat aataaggata atactgaaaa agccgcaaaa
     1081 gcaaacaata ccatacccag tatgaagtgt agtttctcat ttttaaaaaa agaaataaga
     1141 ttcgcaaaaa aacggcttgg ttttttagga gcttttactc cgttttcttg attcttagat
     1201 tgtttactca tataaggggg tgtttttttt cttttgaagt gcaaaggtat ataaaattta
     1261 tctgaacaaa gaaggatttt ttacatagtt tttaacaacg ttttttttat ttttttacat
     1321 atcttaccaa cacaaaacat ataaatctgg acggggattg ataaacataa tggggactgt
     1381 tgcacgtttc aaacaatgca gttctttggg tccaaatagg atatcgtcca acccataatc
     1441 gcgagatgtg cttactaata ccatacccgc ttgcattttt tctgtagtca ttgtggcttc
     1501 tttttctaca ccaaaactat ctgctttagc ttttataatg ctgactttca atgaaaattg
     1561 attgtataat tttagtacac tttctacatt tttggtcgtt tttgaaccat agtcattggc
     1621 ttgatacaaa ataatttctg aatgtaaaaa acgtcccata ttacacgaat aaggggcttt
     1681 ttctttttct tcagtcaaaa aacctattgg aacaataatt tttgataaat caattgggtt
     1741 ggtctggcta tcttttgtaa ttacaaatgg aatacgtagc tctttcagtt gtttaaatat
     1801 gctaagtgga ttgttaaact gcgtgttttt tgaaatctcg aaaaacaaca tcggagtttc
     1861 tgcctgttca gtaaaaggaa ctaattcaga aaaataggat tcggtaaaaa agacttttat
     1921 ttcaatcgac tctttttttt gtagtgtttc tatttgccta ttccatgttt cataaataac
     1981 atcacggtgt gcaagttgct tttttgttaa caataccaaa aaagacagat ttttttcata
     2041 tccttgagca aattgtaagg cacatgcgta ggcattaatt ggaaaataat cggcatcgga
     2101 taccagtaaa aagtgtcgtg tacaagcaat ctcctcaagt ttcatagtgt tcgtcgaata
     2161 tggttagttt ttggtttaca aagttagtgt tttttctttt tcttgtctaa ataaaatgct
     2221 aaaaacatat ttttttgtac ctttgcaact cgcaaaaata ttgtagcgat agtttgtgta
     2281 taaacctatc tagccaagaa aacagtatac atacgactag tattacagag tataacatta
     2341 taatacataa aaatgaaacc tactataccc aaaggtacac g
//
LOCUS       DAOPUR010000234         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_47250_length_2378_cov_3.406802, whole genome shotgun sequence.
ACCESSION   DAOPUR010000234 DAOPUR010000000
VERSION     DAOPUR010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47250_length_2378_cov_3.406802"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PKN90_04820"
     CDS             complement(<1..736)
                     /locus_tag="PKN90_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HNZ85635.1"
                     /translation="MNNKIRVIADCHIPFLQGTIDNIATVTYLAASEISNRTVKDADA
                     LLIRTRTHCNESLLNSSKVQFIGTATIGYDHIDTQYCLQKGIVWKNAPGCNAASVAQY
                     IVAALLYMARKHSLSLTESTIGIIGVGHVGKQVAQMAETLGMRVLLNDPPRQEKEDLP
                     HFVSIHEIAEKANFISYHTPYTNSGVYPTHHLFDQVYCKQLKKKPIIINAARGEIVDT
                     TTLLNALHTRTITDCVLDCWENEPHIS"
     gene            complement(729..>2378)
                     /locus_tag="PKN90_04825"
     CDS             complement(729..>2378)
                     /locus_tag="PKN90_04825"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNZ85636.1"
                     /translation="SISQLLIKEKQATTSYIVEFKASNKGTQFHLYTYEKFNDIRLVY
                     APPTSIALFGEEKDKRSTPKHNANFAVLRVYVSQENQPTPYDPYNKPYNTKQYAKIST
                     RGYEMDDFVVTVGYPSFTNRQITSFALKEQVYAINQAELNVALVKDKVWEHAMRESNY
                     VKNLYEKKYTQNNERLHYLIQLQESVKKQNIIAEKERTEKNFMQWSIKSDFNTLLKYE
                     HVVPTLENKYTSRKQEMYHVHFLTESFVKLDILTVAYMLLTISPQNEREIYSEIAKFY
                     ERYDEQTEKKMLIAMLELYANEATNEFMPDIYTTINSKKYKGNIEQYVNDTFKKSFIT
                     NEVRFNKYLDKPSDKQFNNDPIILLSKSILKKRAEIARIAFADEKEIEHLTALLIEGY
                     QRKNKENTLLADANYTLRMSYGTITTYSPSDGVFFGYTSNFEGIIEKNNTRHAAYKID
                     KRLVDIYQSKHNKNEKPFAVSFLTSIDYTIDKMGAPVFDAEGNLIGILSDGNNETISN
                     IYHYNERFQRTIALDIRYMLFILEHYAGAKRVIEKLNLVDE"
BASE COUNT          625 a          391 c          473 g          889 t
ORIGIN      
        1 cagaaatatg gggttcattt tcccaacaat caagtacaca gtcggtaatg gtgcgtgtgt
       61 gtaatgcgtt gagtagggta gttgtatcta caatttcgcc acgagctgca ttaatgatga
      121 tgggtttttt tttgagttgt ttgcaatata cttggtcgaa aagatgatgt gtagggtaca
      181 cgccactgtt cgtatagggt gtatgatagc tgataaagtt ggctttttcg gctatttcgt
      241 gtatgctcac aaaatgaggt aaatcttctt tttcttgtcg tggtgggtca tttagcaaca
      301 cacgcatacc aagagtttct gccatttgtg ctacttgctt gcccacatgt cctacaccaa
      361 taatgccaat agtactttct gtaagcgata gagaatgttt gcgtgccata tacagcaatg
      421 cagcaacgat atattgagct accgaagcag cgttgcaccc tggagcattt ttccatacaa
      481 tccccttttg aaggcagtat tgggtatcga tatggtcgta gccaatggta gcagtgccaa
      541 taaattgtac cttactagag tttaaaagcg attcgttgca gtgagtgcgt gtgcgaataa
      601 gtagtgcatc ggcatctttc actgttctat tgcttatttc actcgcagct aagtaagtaa
      661 cagtagcaat attgtcaata gtcccctgta aaaatgggat atgacagtca gctattacac
      721 gtattttgtt attcatcaac gaggtttaat ttttcaatca cacgttttgc tccagcataa
      781 tgttcaagaa taaacaacat atatcgtata tcaagggcaa tagttcgctg aaaacgttcg
      841 ttgtagtggt atatattgct gatggtttcg ttatttccat cacttagtat gccaattaaa
      901 tttccttctg catcaaatac gggagctccc attttatcta tagtataatc aatagatgta
      961 agaaaggata ccgcaaaagg tttttcattc ttattgtgtt tgctttggta aatatctact
     1021 aagcgtttgt ctatcttata ggcggcgtgg cgtgtattat ttttttcgat aattccttca
     1081 aaattagagg tatagccaaa aaatactccg tctgaagggc tatacgtagt aatggtacca
     1141 tagctcatac gtagggtgta attagcatcc gctaacaagg tgttttcttt gttttttcgt
     1201 tggtagccct ctattaataa tgcggttaaa tgttcaattt ctttttcgtc agcaaaagca
     1261 atacgggcta tttcggcacg tttttttaat atacttttgc tcaatagaat gattgggtcg
     1321 ttgttaaatt gcttgtcaga gggtttgtca aggtatttgt tgaagcgtac ttcgttggta
     1381 ataaatgatt ttttaaatgt atcgtttacg tattgttcaa tatttccttt gtatttttta
     1441 ctattaatag tagtatatat gtcgggcata aactcgtttg ttgcttcgtt ggcatagagt
     1501 tcgagcatgg caattaacat ctttttctcg gtttgttcat cataacgttc atagaacttt
     1561 gctatttcgc tgtatatttc cctttcgttt tgaggagaaa tcgtcaataa catataggct
     1621 actgtaagta tatcaagctt aacaaaagat tctgttaaga aatggacatg gtacatttct
     1681 tgtttgcggc ttgtatattt attttctaac gtaggtacta cgtgttcata tttaagcaag
     1741 gtgttaaaat cggattttat ggaccactgc ataaaattct tttctgtacg ttctttttca
     1801 gctatgatgt tttgtttttt tactgattct tgtaattgga ttaaataatg tagtcgttcg
     1861 ttgttttgag tatatttttt ttcgtacaag ttttttacat aattactttc tctcatagca
     1921 tgttcccata ctttatcttt cactagggca acattcagtt cggcttgatt gatagcatac
     1981 acctgttctt tcagtgcaaa tgatgtaatc tgtctgttgg taaaacttgg atatcctacc
     2041 gttacaacaa aatcatccat ctcgtatcca cgcgtgctta tctttgcata ttgtttggta
     2101 ttgtaaggct tgttatatgg gtcgtatggg gttggttgat tttcttgcga aacatacact
     2161 ctgagtactg caaaatttgc attgtgtttt ggggtagagc gtttatcttt ttcctcccca
     2221 aatagtgcaa tggaggtagg aggggcatat accaaacgaa tatcattaaa tttttcgtat
     2281 gtgtataaat ggaattgggt tcctttatta cttgctttaa actcaacaat atatgaggtt
     2341 gtagcttgtt tttcttttat taatagttga cttataga
//
LOCUS       DAOPUR010000235         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_47342_length_2375_cov_3.102586, whole genome shotgun sequence.
ACCESSION   DAOPUR010000235 DAOPUR010000000
VERSION     DAOPUR010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47342_length_2375_cov_3.102586"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..757)
                     /locus_tag="PKN90_04830"
     CDS             complement(146..757)
                     /locus_tag="PKN90_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNZ85637.1"
                     /translation="MPIHDTIHTPSLDVAIWHITETADELFSMLLPNPVYTNELNRIS
                     SNKRKSEFLAVRLLMQHLVGDSTIAYLSNGKPILSGSSRFISISHTKEYVAVAISNQH
                     EVGIDIETISAKVHQVKERFMNEIELVHAQQYPEFVLLSWVAKEAAYKKIGDGVVHFS
                     QSMELVPFVPHLSGVLELKAQLLEETRYSFYYQQHTDFVLVYG"
     gene            complement(771..1337)
                     /gene="gldD"
                     /locus_tag="PKN90_04835"
     CDS             complement(771..1337)
                     /gene="gldD"
                     /locus_tag="PKN90_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNZ85638.1"
                     /translation="MNCNIKHILAVGCSIVMVACTNYTPKPMGYFRIALMEKEYAYTQ
                     ANTPYTFEYPTNVARIRPHKNNPLWMNIEYPTYNAKIHCSYLPIKDNFYEISEDTRAF
                     VYKHSVKADAITQQVFENDEAKVYGVLYEIKGNVASSLQFTLTDSTTHYFRGAVYFNN
                     RPNNDSIAPVLSYIREDVVRMMETMQWK"
     gene            complement(1327..>2375)
                     /gene="gldE"
                     /locus_tag="PKN90_04840"
     CDS             complement(1327..>2375)
                     /gene="gldE"
                     /locus_tag="PKN90_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNZ85639.1"
                     /translation="TSILFSLFDFSDSIWLGFLVETVFITLILLLFGENMPKIYAAKY
                     PLRFARFAAPTMRVLIKLLHPFSWLLVKSTSNVKLFDKKKSIKALSASDLSKAYELTS
                     SELEEDKEILEGIIRFSNTEVESIIVPRVDVVAVDITDSFTDIIRIINESGYSRLPVY
                     ADTIDNIKGILFIKDMLAHINKGDEFKWQKHIRPAFFVPTTKHINVLLEDFQKNKTHI
                     AVVVDEFGGTVGVVTLEDILEEIVGDISDEYDDENERSYIQLDTNTFVFEAKILLEDF
                     YKIEGIEKKDFEKIAGEPESLAGLILEIKGEIPQVGDVVLYDNYEFEIVSADKRRIKT
                     IKLTIHAPIQEDEL"
BASE COUNT          734 a          455 c          396 g          790 t
ORIGIN      
        1 cccgcgaccc cctgcgtgac aggcaggtat tctaaccagc tgaactacca caccgtgtgt
       61 ttcgctcaat tgcgatgcaa agatatgctg attttttata ttatacaagt gttgatggta
      121 tttttttgat aaaatcgaca aaaaattacc catataccaa tacaaagtca gtatgttgct
      181 gataataaaa ggaataccgt gtttcttcaa gtaattgtgc ctttaactcc aacacgcctg
      241 ataaatgcgg aacaaacggc acaagctcca tagactggga aaaatgcacc accccgtcgc
      301 ctatcttttt gtatgcagcc tctttagcta cccaagataa caagacaaat tcggggtact
      361 gttgagcatg tacaagctct atttcgttca taaaacgttc ttttacttga tgtaccttgg
      421 cagaaattgt ttcaatatca atgcctactt catgttgatt gcttatggca actgcaacat
      481 actcttttgt atgcgaaata ctaataaaac gcgacgaacc agataaaata ggtttaccat
      541 tcgataaata ggcaatagtg ctatccccta ctagatgttg cattaataaa cgcactgcca
      601 aaaattcgga ctttcgttta ttgctagata tcctgttaag ttcgtttgta tacacaggat
      661 tgggcaatag catcgaaaac agctcgtcag cagtttcggt tatatgccaa atagctacat
      721 ctaggctagg tgtgtgtatg gtatcatgaa taggcacttt ggtcgaaata ttatttccat
      781 tgcatggttt ccatcatgcg tactacatct tcgcgaatgt aagacaaaac gggagcaata
      841 gaatcgttat tgggtcggtt gttaaaatag acagcacctc taaaataatg ggtagtacta
      901 tcagtcagag taaactgcaa ggaagaggct acattgccct taatttcgta caacacccca
      961 tatacctttg cttcgtcatt ttcaaaaact tgctgggtaa tggcatctgc ttttactgaa
     1021 tgtttgtaga caaaagctct ggtatcttcg gatatttcat aaaaattatc ctttattggc
     1081 aaataactac aatgaatttt tgcattatat gtaggatatt ctatgttcat ccacaatggg
     1141 ttatttttat gtggacgaat gcgtgcaaca ttggtaggat attcaaacgt atagggcgta
     1201 tttgcttgtg tatatgcata ttccttttcc attagtgcta tccgaaaata gcccattggt
     1261 ttgggagtgt agttggtaca agctaccatt acgatactac atcctactgc tagaatatgt
     1321 tttatattac aattcatctt cttgaatagg tgcatgtatg gttaatttaa tcgttttaat
     1381 gcgtcttttg tctgccgata caatttcaaa ttcgtagtta tcatacaaaa caacatctcc
     1441 tacttgcggt atctcccctt ttatttctaa gattaaacct gccaaacttt ctggttcacc
     1501 tgctattttt tcaaaatcct ttttttctat accttcaatt ttgtaaaagt cctcgagtag
     1561 aatctttgct tcaaacacaa atgtatttgt atctagttga atataagagc gttcattttc
     1621 atcatcatat tcatcgctaa tatcgcctac tatttcttct aaaatatctt cgagggtaac
     1681 aacacctaca gtaccaccaa attcatctac aactacggca atatgtgttt tatttttctg
     1741 aaaatcttcg agtaatacat tgatgtgttt tgtagttgga acaaaaaatg caggtcttat
     1801 gtgtttttgc catttaaact catctccctt gttgatatga gccaacatat ctttgataaa
     1861 caaaataccc ttaatattgt caatagtgtc tgcataaaca ggtaaacgcg agtaacctga
     1921 ctcattaatt attcgaataa tatcagtaaa cgaatctgtt atatccactg ctactacatc
     1981 tacccttggc acaataatac tctctacttc tgtattacta aaacgtataa ttccttctag
     2041 aatttcttta tcttcttcta attcggatga agtaagttcg tatgctttag ataaatcact
     2101 ggctgacaat gctttgattg attttttttt atcaaataac ttcacattgc tggtagattt
     2161 gaccaacagc caggaaaaag ggtgtaatag tttaattaat acacgcatag tgggtgcggc
     2221 aaatcgtgca aaacgtaatg gatattttgc cgcataaatt ttgggcatat tctctccaaa
     2281 taacaataga ataagtgtaa tgaatactgt ttcgactaag aaacctaacc atatggaatc
     2341 cgaaaaatca aacaacgaaa aaagaatact agtag
//
LOCUS       DAOPUR010000236         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_47498_length_2370_cov_3.596544, whole genome shotgun sequence.
ACCESSION   DAOPUR010000236 DAOPUR010000000
VERSION     DAOPUR010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47498_length_2370_cov_3.596544"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1381)
                     /gene="nqrF"
                     /locus_tag="PKN90_04845"
                     /pseudo
     CDS             complement(120..1381)
                     /gene="nqrF"
                     /locus_tag="PKN90_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
     gene            complement(1422..2021)
                     /gene="nqrE"
                     /locus_tag="PKN90_04850"
     CDS             complement(1422..2021)
                     /gene="nqrE"
                     /locus_tag="PKN90_04850"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNZ85640.1"
                     /translation="MENLSSLFVKSIFVDNMIFAYFLGMCSFLAVSKNVKTAFGLGVA
                     VTFVQVMTLPVNYLLENYILKPDVLIEGVDLSFLSFIVFIAVIAGITQLVEMMVEKFT
                     PALYASLGIFLPLIAVNCAIMGGSLFMQQRNFTSISEASVFAVGSGLGFLVAIVGLAA
                     IREKMAYSHVPAPLKGLGITFIVVGLMAMAFMCFSGLKV"
     gene            complement(2047..>2370)
                     /locus_tag="PKN90_04855"
     CDS             complement(2047..>2370)
                     /locus_tag="PKN90_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNZ85641.1"
                     /translation="SVYVGLIITNCILMGRLEAFAMSNRPWESFLDGLGNGFGYALIL
                     VIVAFFRELLGSGSLLGFHVIPASVYESGYVNNGLMVMPPMALIILAIIIWAHRTKVK
                     QLQDN"
BASE COUNT          866 a          480 c          322 g          702 t
ORIGIN      
        1 aaaagatttt tgtttgctat ataccttcaa cataacagat atgaaataat acaatgagtt
       61 aaaaacaaaa gagactatct ttacgatagc ctcttagtaa aaaatatgcg ttcaattatt
      121 taagcaccaa aatcatcgaa cattagcatt tcgcgaggaa cacctaagtc gtataacatt
      181 ttttcaacag catttgccat tggtccaggt ccacacatgt aatactcaat atcttctgga
      241 gcctcatgtt tacttaggta ctcattcaat atcacactat aataaatccg acataacctg
      301 tccaattgtc ttcaggttga ggatcactta atgcaatatt aaatttgaaa ttagggaatt
      361 ctttctctaa tgctcgaaaa tcttcttcgt agaatatttc gtttttagaa cgagcaccat
      421 accaataaga aatctttcta tccttaattt tcaacgtatt aagaagatgc aaaatgtgag
      481 aacgtaaagg agccatacca gcacctccac cgatatacaa catttctcta ccagtgtcaa
      541 gtagaggatg aaactctcca taaggacctg aaacaattac tttatcgcca ggttttagtg
      601 aaaagatata cgatgacgca ataccaggag gcacattcaa aaaaccccca ttaattctat
      661 caaaaggtgg tgtagcaata cgaatattta acatcacaat atttccttca gcaggataat
      721 ttgccataga atacgcacgt actgtttcct cagtattggt acatgttaaa ttccacatat
      781 taaatttatc ccaatcaccg cgaaatctat cttgaatatc aaaatcagaa aacttaatcg
      841 cgtatttggg tatattaatt tgaatataac ctccagaaat aaagtttaaa ttttctcctt
      901 cgggcaattt aacaacaaat tctttgataa aagtagccac gttattgtta gaaacaacct
      961 cgcattccca ctgcttcact cctaagatag cttcatcaat tttgatttgc atgttgtttt
     1021 ttacctttac ttgacaacct aaacgccaat tatcttttac ttctttacga gaaaagtgaa
     1081 ctgtttctgt tggtaaaatt tcaccaccac catcaataac ctgacattta cactgagcac
     1141 agctaccacc tcctccacac gcagatgata aaaatatctt ttcatttgcc aatgtagaca
     1201 ataatgtaga tccagattca acttctatct ctttttctcc gttaattaaa attttaacag
     1261 gacctgatgg agataaatat tttttagcaa ataataagat tactacgagt aaaagaatta
     1321 ccgctaaaaa taccactata ctagtgccta tcataatagg acttacagat aaaaatgtca
     1381 tatagttatt tttttaaatg ctgttttgta tcttaaattt attagacttt taagccagaa
     1441 aaacacatga atgccatcgc catcaaccct actacgataa atgtaattcc caagcctttt
     1501 agaggtgctg gtacatgtga atatgccatt ttttctctaa ttgcagctaa tccaacaatt
     1561 gcaacaagga aacctaaccc cgaacctact gcaaaaacag atgcttcgct aatggaagta
     1621 aaattacgtt gttgcataaa caaagagcca cccatgatag cacagtttac agcaattaaa
     1681 ggtaagaaaa ttcctaaaga agcatataga gcaggagtaa atttctccac catcatttct
     1741 accaattgtg taatacccgc gataacagca ataaagacga taaaacttaa aaaactcaaa
     1801 tctacccctt cgattaaaac atcgggttta agtatatagt tctccaataa atagttaaca
     1861 ggtaaggtca taacctgcac aaacgttaca gctacaccta gaccaaaagc agttttaaca
     1921 ttcttagaaa cggctaaaaa agaacacatt cccaagaaat aggcaaaaat catattatcg
     1981 acaaaaatcg acttgacaaa taagcttgat aaattttcca ttcgttctga atttactagg
     2041 ttgtaattaa ttatcttgta attgttttac ttttgtacga tgagcccaaa taataatagc
     2101 taaaataatc agtgccattg gaggcattac cattaaacca ttattgacat aacccgattc
     2161 gtaaacactt gcagggataa catggaatcc aagtaatgaa ccagaaccaa gcaattcgcg
     2221 aaagaaggct acaataacca aaattaatgc ataaccaaac ccattaccca atccatctaa
     2281 aaaagattcc cagggtctat tagacatagc aaatgcttct aaacgtccca tcaatataca
     2341 gttagtaata attaatccta catataccga
//
LOCUS       DAOPUR010000237         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_47656_length_2365_cov_3.148485, whole genome shotgun sequence.
ACCESSION   DAOPUR010000237 DAOPUR010000000
VERSION     DAOPUR010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47656_length_2365_cov_3.148485"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1570)
                     /locus_tag="PKN90_04860"
     CDS             complement(74..1570)
                     /locus_tag="PKN90_04860"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNZ85642.1"
                     /translation="MNTKNVKWLWIAVATFVVSVAVATLISFFIVKNHGLGNQAFLSS
                     KQHAPSTFVAMSEDSPIASHGFVKAAEATVNTVVHVRTKSPAKQVAFSRDPFFEFFFG
                     YPQMERQEPRMQEGAGSGVIISSDGYIVTNSHVVASSTNIEVTLNDKRTFEATLIGSD
                     PNTDIALIKIDAKDLPVSVFGDSDALSVGEWVLAVGNPFNLTSTVTAGIVSAKARSIN
                     ILSGDMKIESFIQTDAAINPGNSGGALVNLQGELVGINTAIATTTGAYAGYGFAVPSS
                     IVQKVVVDLKQYGTVQRAVLGVQIADITSELAKEKGLKTLDGVYIHSVVENGAADNAG
                     LKSEDIIQQINGIRVNTVAQLQEQLSRFRPGDQVTIQVLRKNKELSMRATLKNKLGNT
                     DVVGSQVNAEEYLTIRVKEIDNQTKSQLRLSYGLQITEIKKGKLFDQGIKNNYIIVSV
                     NNQAIRTKDDLQQALDTALNAADRNKVLFITGVYPNGVRAYYAINLDE"
     gene            complement(1757..>2365)
                     /locus_tag="PKN90_04865"
     CDS             complement(1757..>2365)
                     /locus_tag="PKN90_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ85643.1"
                     /translation="EGFTDIIINVHHFADQIIQFLAQNQHFGLNIAISDETQQLLETG
                     GGIKKASWFFGNEPFLIHNVDILSNLPLAELVRFHTNKPDAAATLVVSKRTTTRYLLF
                     DNDMQMKGWTNIQTNEIKSSYPQTIIQACSEFAFSGIHVFSPALFPYFEAYPAKFSVI
                     DFYIDVCGKAAVYGYVPTNLQLLDVGKIDSIQQAELFVQTMY"
BASE COUNT          695 a          496 c          436 g          738 t
ORIGIN      
        1 tactccgaaa caataaaagc gaataaccca cataggttac tcgctttata tatttaatca
       61 ttcagactgg ttattactcg tccaaattaa tggcatagta ggctcttact ccattgggat
      121 atacgccagt aataaataat accttattgc gatcggcagc atttagggct gtatctaggg
      181 cttgttgcaa atcgtctttg gtgcgaatag cttggttgtt taccgacaca atgatgtagt
      241 tgttttttat accttggtcg aataatttac cttttttgat ttcggtaatt tgcaaaccat
      301 acgacaacct taattgagat ttagtttgat tatcaatttc ctttacccgt atcgtaagat
      361 attcttcggc attcacttgc gaacccacta cgtctgtgtt gccaagttta tttttaagag
      421 tagccctcat acttaattct ttattctttc taagtacctg tatagtaacc tgatcgcctg
      481 gtctaaagcg acttagttgt tcttgcaatt gagccaccgt atttaccctt atgccgttaa
      541 tttgctgaat aatgtcttcg gatttaagtc ctgcattatc ggctgctcca ttctctacta
      601 ccgaatggat atatacacca tctagggttt ttaagccttt ttctttggct aattcgctgg
      661 taatatcagc aatttgaaca cccaacacag cacgttgcac agtgccatat tgttttaaat
      721 ctacgactac tttttgcaca atactggatg gcacagcaaa cccataacca gcatacgctc
      781 cagtcgttgt agcaatagcg gtattaatac ctaccaattc cccttgtaaa ttcaccaacg
      841 cacctccgct attccctgga ttgatggctg cgtctgtttg gataaatgat tcaattttca
      901 tatcgcccga caagatattg atgctgcgtg ccttggcact aacaatacct gccgttacgg
      961 tagaagtgag gttaaacgga ttacccacag ccaataccca ctcacctaca ctaagtgcat
     1021 cggagtcgcc aaatacggac accggtaagt ctttggcatc aatttttatc agggcaatat
     1081 cggtattggg gtcagagcca attaaggtcg cctcaaaagt acgtttatca ttcaacgtaa
     1141 cttctatatt ggtagaactt gctaccacat ggctattggt tacaatatac ccatcagaag
     1201 agataataac ccccgaacct gctccttctt gcatacgtgg ctcttggcgt tccatttggg
     1261 gatatccaaa aaagaattca aaaaatgggt cgcgcgaaaa agcaacttgc ttggcaggcg
     1321 attttgttcg tacgtgcacc actgtattta cggtagcctc ggctgctttt acaaaaccat
     1381 gcgaggcaat gggactatcc tccgacatgg cgacaaaggt ggacggagca tgctgtttgc
     1441 ttgataaaaa agcttgattg cccaatccat gatttttgac aataaaaaaa ctgatgaggg
     1501 ttgctactgc tacactgact acaaaagtag ccaccgcaat ccaaagccat tttacatttt
     1561 ttgtgttcat acttgttatt attaaattgt tttttattgg gtttgtttat gctcaaaaat
     1621 aaacaaaaaa tacgccaata agaccttgta tcactaggtt ttatcaattt ttaatacttt
     1681 gattttgggc attaacgggt attaacccag caggcagaaa tcgtaaaaaa tttttaatac
     1741 ataatctgac aaaaaatcag tacatagttt gcacaaacaa ttcagcttgc tgaatactgt
     1801 caatttttcc tacatctaac aactgcaaat tggtaggcac gtagccatac acagcagcct
     1861 tgccacatac atcaatgtaa aaatcaatta cagaaaattt agcggggtaa gcctcaaaat
     1921 agggaaataa tgcgggcgaa aacacatgaa tgccactaaa tgcaaactca ctacaggctt
     1981 gtataatagt ttgtggataa gaagatttta tttcgttggt ttgtatattc gtccaccctt
     2041 tcatctgcat atcattatcg aacaacaagt aacgcgttgt agtacgttta cttaccacca
     2101 aagtggctgc cgcatcgggt ttattggtat gaaatcgtac aagttcagct aagggaagat
     2161 tcgacaagat atctacgttg tgtatcaaaa aaggttcgtt gccaaaaaac caagatgctt
     2221 ttttgatgcc tcctcctgtt tcgagtaatt gttgtgtttc gtctgagatg gcaatgttga
     2281 gtccaaaatg ttggttttga gccaaaaact gaattatctg atcagcaaaa tgatgcacgt
     2341 tgatgataat atccgtaaaa ccctc
//
LOCUS       DAOPUR010000238         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_47844_length_2358_cov_3.386452, whole genome shotgun sequence.
ACCESSION   DAOPUR010000238 DAOPUR010000000
VERSION     DAOPUR010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47844_length_2358_cov_3.386452"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PKN90_04870"
     CDS             complement(<1..973)
                     /locus_tag="PKN90_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85644.1"
                     /translation="MKFFTIFICSIMYCVSVLADASISIATLRQSNDNNPYLQSEVAV
                     EGVVTGVFQQSNQIGGFFIQDEKGIFVEHTTKKVSIGDSVRITATLTNNNNRLQLTNV
                     SALDIVNSNNQIPITKLIFPDDFDSLEAWEGTLVELDQTMYVTNNYNLFRYGQLTLSA
                     NRLYIPTHQALPLSDVYFQTISSNEINQLILDDASSVSYPTNKPFTDSNGTRRMGEKI
                     DNLQVIIDQVGTNYVVYAPKDIDFYGNPRTATHPSIGDYSIKVCGTNLEMFKTSYWGD
                     GFGADNSTEFARQRTKILAALHAINADIYAITEIQQGSNALENLVNGL"
     gene            1091..1543
                     /locus_tag="PKN90_04875"
     CDS             1091..1543
                     /locus_tag="PKN90_04875"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNZ85645.1"
                     /translation="MSYPLVGLNKIYVLFVFTLCSVSTMAQSYLGVWEQINNDGQKGA
                     LVRIEKSSRANTYVGTIIHVFRYPQDILCTHCKGDLHNKPVVGMQFMYDMEYKKGELV
                     NGKIIDVKTGKTYYTKLSYNKQDDALLMRASVDASGFIGETQVWKRYK"
     gene            complement(1556..>2358)
                     /locus_tag="PKN90_04880"
     CDS             complement(1556..>2358)
                     /locus_tag="PKN90_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ85646.1"
                     /translation="ATSNDVIVSQDIAKTLQLKVNDRFLCYFVKDGIRVRRFTVKGIY
                     NSNFSEFDKLFILADLRHIQRLNGWEENEITGVEILVTDYRKLDDITDAVFMQTANRF
                     EEKGNTYYTRSIKQLQPQIFGWLDLLDMNVWVILILMLTVAGFSMISGLLILILERTN
                     MIGILKALGGKNWFIRKIFLYQAFFLVGKGMLWGNILGISLALLQQQFNIITLDPLTY
                     YVNTVPINLNLWYIVFVNALCLFFSLLVLLAPSHMIAKIEPSKAIRFE"
BASE COUNT          783 a          413 c          390 g          772 t
ORIGIN      
        1 taagtccatt cactaggttt tccaatgcgt ttgacccttg ctgaatttct gttatggcat
       61 aaatatctgc atttattgca tgcaaagctg ctaaaatttt tgttcgttgg cgtgcaaact
      121 cggtactatt atcagctcca aacccatctc cccaataaga cgttttaaac atttctaaat
      181 ttgtgccaca aacttttatt gaataatctc caattgatgg atgcgttgct gtacgtggat
      241 tgccataaaa atcaatgtct ttcggagcat acactacata atttgttcca acttggtcga
      301 taatgacttg caagttatca attttttcac ccattcgtcg tgtgccattg ctatctgtaa
      361 aaggcttatt tgtaggatac gatacagaag atgcatcatc tagtattagt tggttaattt
      421 cgttactcga aatagtttga aaatacacat cacttaatgg gagtgcttga tgcgttggaa
      481 tgtataaacg gttagcagat aaggtaagct gtccgtatcg aaaaaggttg tagttgttgg
      541 taacatacat ggtttggtct aattcaacga gagttccttc ccatgcttcc agtgaatcaa
      601 aatcatcagg aaaaattaat tttgtaatgg gtatttggtt gtttgagttt acaatatcta
      661 aagcagatac attcgttaat tgtaatctgt tattgttgtt agtgagtgtg gcagttatac
      721 gtacactatc tccaatgctt actttttttg tggtatgttc aacaaaaatt cctttttcat
      781 cttgtataaa aaaacctcca atttgattac tttgttgaaa cacacccgta acaacgcctt
      841 ccaccgctac ttcagattgt aaatagggat tattatcatt cgattgtctt aatgtagcaa
      901 tggatatact tgcatcagcc aagactgaaa cacaatacat gatagaacaa ataaagattg
      961 taaaaaattt cattaaaatg ataattaata aaaaaatgcg aactacaaag atactttttt
     1021 tgagtagaaa ttgataatgt tcttaataat ttatagtttt gtaattagta aaatttctaa
     1081 ctagtagtgt atgtcgtatc cacttgtagg tttaaacaaa atatatgtcc ttttcgtgtt
     1141 tacactatgc agtgtatcaa ctatggctca atcttatctt ggtgtttggg aacaaataaa
     1201 taatgatgga caaaaaggag ctttggtgcg tatagaaaaa tcatctcgag caaacaccta
     1261 tgttggaact attattcatg tttttcgcta tccacaagat attttatgta cccattgtaa
     1321 aggcgacttg cataacaaac cagttgtagg catgcaattt atgtacgata tggaatataa
     1381 aaaaggagag ctcgtaaatg gtaagattat tgatgtaaaa acaggaaaaa catattacac
     1441 taaactatct tacaataaac aagatgatgc cctgctaatg agagcttcgg tcgatgcatc
     1501 tggttttatt ggagaaacac aggtttggaa acgctataaa tagtcttcat attcattatt
     1561 cgaaccgaat agcttttgat ggctcaattt tggctatcat atgcgatgga gctaataaca
     1621 ccaaaagaga aaagaataag caaagagcat tcacgaatac tatgtaccac agattgagat
     1681 tgataggaac ggtatttaca tagtaagtaa gaggatcgag ggtaatgata ttgaattgtt
     1741 gttgtagcaa agccaaacta atccccaata tatttcccca aagcatgcct ttacctacta
     1801 agaagaacgc ttgatataaa aatattttac gaataaacca gtttttaccg cccaaagctt
     1861 tcaatatgcc aatcatattg gtacgttcca agattagaat taacaagccc gatatcatac
     1921 taaaaccagc caccgtaagc atcagtatta aaattaccca cacgttcata tctagcaaat
     1981 cgagccaacc aaatatctga ggttgcaatt gtttaatgct acgagtgtag taggtattcc
     2041 ctttttcctc aaaacgattc gccgtttgca taaatactgc atccgtaata tcatcgagct
     2101 ttcgataatc tgtaaccaat atttctacac cagtaatctc attttcttcc caaccattta
     2161 atcgctgtat atgacgtaaa tcagctagta taaacagttt atcaaattcg ctaaaattag
     2221 aattatatat tccttttacc gtaaaacgcc ttacccgaat accatctttc acaaaataac
     2281 ataggaatcg atcatttacc ttcaattgta atgttttggc aatatcttgc gatacaatta
     2341 catcatttga tgtagcac
//
LOCUS       DAOPUR010000239         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_47989_length_2353_cov_3.960400, whole genome shotgun sequence.
ACCESSION   DAOPUR010000239 DAOPUR010000000
VERSION     DAOPUR010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47989_length_2353_cov_3.960400"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2353
                     /gene="sprA"
                     /locus_tag="PKN90_04885"
     CDS             <1..>2353
                     /gene="sprA"
                     /locus_tag="PKN90_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNZ85647.1"
                     /translation="NYYPGGDCVDLIGIDGYRTISLVNNALGQSLSGFGIVGGEDYVK
                     IESARRLEPTEYSLNKQLGFISLRRTLGTDEVLAVAYEYTYNGKVYQVGEFSTDGIES
                     PNVLTLKLLKGVSQSPNVPMWDLMMKNVYSLGAFQVQKDKFRLDVMYRSDSTGVDLNY
                     IPKGKIANTILLRVMNLDRLDSRSQQNPDGVFDFVENFTVIPENGRIIFPVVEPFGSH
                     LRKAFDDDVVANEFVFQELYDSTIVVAKEVAEKNKFRLVGQYRSSSGSEIRLNAMNVP
                     QGSVKVTAGGRQLTENTDYTVDYMMGVVNILNQDIIESGTPISINLENKSLFSMQRKT
                     LVGTHLDYAFSDKFNVGGTFMHLSEKPLTQKVLMGNEPISNTIWGLNTSYTTQSQWLT
                     NLLDKIPLLNLTEPSSINLNAEFAQMIPGHSKTIDEQGIAYLDDFESTKIAFDIRHPQ
                     MWGLASTPYHPTGGLFPEAALVNDIEYGKNRALLAWYYIDPIFTRNTSRTPLHIKNDK
                     EQLSNHFVREIREREIFPNRQLLYGQSGYIQTLNVAYYPTERGPYNIYPTSYDSEGKL
                     TNPKGKWGGMMRRLETTDFENNNIEYMEFWLLDPFVYDTLHVMKGGDLYVNLGDVSED
                     ILKDGRKFFENGLPVTDAQPQAETTNWGKVPSRQAVVYAFDNEANARKLQDVGLNGLT
                     TNEEFTFGAYQTFINQLKATLTPEALQKFENDPFSPLNDPAGDNFSHYRSSYYDNQQK
                     GVLERYKRYNGTEGNSPAQADTNEDFTTSATNLPDVEDINNDNT"
BASE COUNT          765 a          391 c          493 g          704 t
ORIGIN      
        1 taaactatta tccgggcgga gactgtgttg atttgatagg tattgatgga tatcgtacta
       61 tttctttggt aaataatgca ctgggacaaa gtttgtcagg ttttggtatt gtgggaggag
      121 aggattacgt aaaaattgaa agtgctcgac gattagaacc aacggaatat tcattaaaca
      181 aacaattagg gtttatttcg ttgcgacgca cattaggcac agatgaggta ttggctgtag
      241 catacgaata tacatacaat ggaaaagtat accaagtagg ggaattttct actgatggta
      301 ttgagtcgcc taatgtgtta acactgaaac ttctaaaagg tgtttcccaa tcgccaaacg
      361 tacctatgtg ggatttgatg atgaagaatg tatattcgct tggagcattt caagtacaaa
      421 aagacaaatt ccgtttggat gtaatgtacc gtagtgattc tactggagtg gatttaaact
      481 atatccccaa aggaaaaata gccaatacaa ttttattgag ggtgatgaat ttagaccgat
      541 tagactctcg ctctcaacaa aatccagacg gagtgtttga ttttgttgaa aattttactg
      601 ttattcccga aaatggtcgt attatatttc cggttgttga gccttttggc tctcatttgc
      661 gtaaagcttt tgatgatgat gtggtagcaa acgaatttgt gtttcaggag ttgtacgatt
      721 ctactattgt tgtagccaaa gaagtggcag aaaaaaataa atttcgcttg gtggggcaat
      781 accgctcttc ttcgggatcg gaaattcggt taaatgcaat gaatgtgcct caaggatcag
      841 taaaggtaac tgctggtggt cgtcaactaa cagaaaatac tgattataca gtagattata
      901 tgatgggggt tgtcaatatt ttgaatcaag atattattga gtcaggcaca ccaataagta
      961 ttaatttgga aaataaatcg ctctttagta tgcaacgtaa gaccttggtg ggaacccatt
     1021 tggattatgc atttagcgat aaatttaatg taggaggtac ttttatgcat ttgtcagaaa
     1081 aacctcttac ccaaaaggta ctcatgggga atgaacctat ttcaaataca atctgggggc
     1141 taaatacttc ttatactacc caatcccaat ggttaactaa tctgttagat aaaattcctt
     1201 tgctcaattt aacagagcca tcttctatca atttaaatgc agagtttgct caaatgattc
     1261 ctggacattc caaaacaata gatgaacaag gaatagctta tttagatgat tttgaatcta
     1321 ccaaaatagc gtttgatatc cgtcatcctc aaatgtgggg gctagctagt actccttatc
     1381 atccaacagg tggtttattt ccagaggcag ccttggtaaa tgatattgag tatggcaaaa
     1441 atagagcctt gcttgcctgg tattacatcg acccaatttt tacacgcaat acttctcgaa
     1501 cgccattgca tattaaaaat gacaaagaac agctatcaaa tcattttgtg cgtgaaatca
     1561 gagagcgtga aattttccct aatcgacaat tgttgtatgg acaatcgggg tatattcaaa
     1621 cattaaatgt agcgtattat cctactgagc gtggaccgta taatatttat ccaactagtt
     1681 atgactcaga agggaagtta actaacccaa aagggaaatg gggtggtatg atgagacggt
     1741 tagaaacaac cgattttgaa aacaacaata tcgaatacat ggagttttgg ttgctcgatc
     1801 cctttgtgta cgatacttta catgtgatga aaggtggaga tttatatgtc aatttaggag
     1861 atgtttcgga agatatttta aaagatggca gaaagttttt tgaaaatggc ttacccgtaa
     1921 ctgatgctca gccacaagct gaaacaacca attggggaaa agtgccatca cgtcaagcgg
     1981 tagtgtatgc atttgacaat gaggctaatg cacgaaaatt gcaagatgtt ggattgaatg
     2041 gattaactac caacgaagag tttacttttg gggcttacca aacttttatt aatcaattga
     2101 aggcaacatt aacgccagaa gccttacaga aattcgaaaa cgatccattt tctcccctca
     2161 acgatccagc aggggataat ttttcgcatt atagaagctc gtattacgat aaccaacaaa
     2221 aaggagtatt agaacgctac aaacgctata acggaactga aggtaattct cctgcacagg
     2281 ctgatacaaa cgaagacttt accacgtcgg ctaccaattt gcctgatgta gaagacatta
     2341 ataatgataa tac
//
LOCUS       DAOPUR010000240         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_48575_length_2334_cov_2.846863, whole genome shotgun sequence.
ACCESSION   DAOPUR010000240 DAOPUR010000000
VERSION     DAOPUR010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48575_length_2334_cov_2.846863"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PKN90_04890"
     CDS             <1..1291
                     /locus_tag="PKN90_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNZ85648.1"
                     /translation="KNIQVPVLDEFGDPVINEAGNPVMQPVKDTLYAEGCHEPTKMGV
                     VPAYTVDKKGFDCDDYISIGKLGEYAYRLELYRRNCQIGTKFEFVIERSGDIPTLSLN
                     GVNPICDGSSEVIKATIVATPATTTYKWILNETTIATTKDLTRVFTMDDNQGKLKFEA
                     KNTCGTVVSNEIALSVLPRPTGIVTTEKGYLCQGQSSNVTAFLAGKAPFTYTLTGNTE
                     QTTSNVSETVSMSPTSTIDITFESLSDANCVATPADITGTSITVYPAPSIDITVNVPS
                     PMVSGRYVTATVTPGFVDYSLWINNQEIEALATEENVFKSKNFVYGLSSNIFDTKVED
                     TNGCIWDFSKTEDITVVLFPNVFTPNNDGVNDVFLEGYNLKVFDRLGTVLYSGTKGWN
                     GIYNGVPVNKGVYLYTVDILDENGDTVIIKNTVVVEK"
     gene            1318..>2334
                     /locus_tag="PKN90_04895"
     CDS             1318..>2334
                     /locus_tag="PKN90_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85649.1"
                     /translation="MKITKKSFLFALLVCLPIISMMGNMKVGNNKYERRDYLGAIEQY
                     KKTAEGNSNEKTVANAKYKIGECYSLLNEPKEAVSYLGEAIAGGVPTLEAYVLYGKNL
                     LKMGEYARAKASFEEFDRLSPKDARIKNLIASCDFSLKHSSKNPITPEQDLKGINTSE
                     IEYGIGFYQDGIVYASVEQAKKGLSSRMYFSHGKDNFTSSRLVDMIKIRTGQNVGTFA
                     IDTLDNILYYTRCNNNERDNCYIYTARYKKGKWKEKGALNIGNRHANVAHPALSPDRQ
                     RLYFTANLEGGFGGTDIWYVTRNENGKWSKTPINVGSVVNTAGNEAFPYVVGNTLIFA
                     SEGHI"
BASE COUNT          815 a          420 c          456 g          643 t
ORIGIN      
        1 aaaaaacatc caagtacctg tgttagatga atttggagac cctgtaataa acgaagctgg
       61 taatccagta atgcaacctg taaaagatac attatatgca gaaggttgcc acgaaccaac
      121 taaaatggga gtagtgcccg cttataccgt agataaaaaa ggatttgatt gcgatgatta
      181 tatttctatc gggaaactag gagaatatgc gtaccgtttg gagttatata gaagaaattg
      241 tcaaataggt acaaaatttg aatttgttat cgaacggagt ggtgatattc ctaccttatc
      301 attaaatgga gtgaatccga tatgcgatgg ttcttctgaa gtaataaaag caacgatagt
      361 agcaactcct gccactacta cttacaaatg gatattaaac gaaacaacca ttgctactac
      421 caaagatttg acccgcgtat ttacgatgga tgataatcaa gggaaactta aatttgaagc
      481 aaaaaatacc tgcggaacgg ttgtttctaa cgaaattgcc ttgtctgttt tacctcgtcc
      541 cacaggtatt gtaactaccg agaaaggata cctttgtcaa ggacaatcat caaacgtaac
      601 agccttttta gctggtaaag ctccatttac gtacaccttg actggcaaca cagaacaaac
      661 cacctctaac gtgtcagaaa cagtgagtat gagtcctacc tctactattg acattacatt
      721 tgagtcgctt agcgatgcca attgtgttgc tacacctgct gatatcacag gcacaagcat
      781 taccgtatat cctgcacctt caatagatat tacagtaaat gtaccttcac cgatggttag
      841 cggacgttac gttacagcta cagtaacacc tgggtttgtg gattactctt tgtggataaa
      901 taaccaagag atagaagcat tagcaaccga agaaaatgtg tttaaatcaa aaaacttcgt
      961 gtatggactt tcatcgaata tttttgacac caaagttgaa gataccaatg gctgtatctg
     1021 ggatttttct aaaacagaag acattacagt tgttcttttc ccaaatgtat ttacgcctaa
     1081 caacgatgga gtaaacgacg tatttcttga aggatataac ctaaaagtat tcgaccgatt
     1141 aggcaccgta ttgtactccg gaacaaaagg gtggaatggg atatacaatg gtgttccagt
     1201 aaacaaagga gtgtacttat atacagtgga tatactcgac gaaaatggag atactgtgat
     1261 tattaaaaat actgtagtag ttgaaaaata agtgtaaacg aaataaaagt acattatatg
     1321 aaaataacta aaaaaagttt cctatttgcc ttgctggtat gcttgccaat tatcagtatg
     1381 atgggtaata tgaaagtcgg aaacaataaa tacgaaagac gcgattactt aggtgcaatt
     1441 gaacaataca aaaagactgc tgaaggcaac tcaaacgaaa aaacagtagc caatgctaaa
     1501 tataaaattg gagaatgtta ttccctatta aatgaaccga aagaagctgt ttcttatcta
     1561 ggagaagcca ttgctggagg tgttcctaca ttggaagcat acgtgctata cggtaaaaac
     1621 ttactcaaaa tgggagaata tgcacgtgct aaagcttctt ttgaagaatt cgacagactt
     1681 tcgcccaaag atgctcgtat taaaaactta attgcttcgt gcgatttttc ccttaaacac
     1741 agctcaaaaa atcctattac tcccgagcaa gacttaaaag gaatcaatac ctctgaaatt
     1801 gaatacggaa ttggttttta tcaagatggt atcgtatatg cctctgttga acaagcaaaa
     1861 aaagggctat catcacgtat gtatttttcg catggtaaag ataatttcac ctcatctcgc
     1921 ttggtggata tgattaaaat acgaacaggt caaaatgtag gtacatttgc catcgacaca
     1981 cttgataaca tcttatatta cacccgttgt aacaataacg aaagagacaa ttgctacatc
     2041 tacacagccc gttataaaaa agggaaatgg aaagaaaagg gagctttaaa tattggcaac
     2101 agacatgcga atgttgctca ccctgccctt tcgcccgaca gacaacgctt gtattttaca
     2161 gcaaatttag aagggggatt tggtggaaca gatatttggt acgtaacgcg taacgaaaac
     2221 ggaaagtgga gtaaaactcc tatcaatgtt ggatccgtag taaatacagc tggcaacgaa
     2281 gctttccctt atgtagtagg taatacgctc atatttgcat cagaaggaca tatt
//
LOCUS       DAOPUR010000241         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_48723_length_2329_cov_3.124450, whole genome shotgun sequence.
ACCESSION   DAOPUR010000241 DAOPUR010000000
VERSION     DAOPUR010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48723_length_2329_cov_3.124450"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1794
                     /locus_tag="PKN90_04900"
     CDS             217..1794
                     /locus_tag="PKN90_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNZ85650.1"
                     /translation="MTFQEEIKRRRTFAIISHPDAGKTTLTEKLLLFGGAIHIAGAVK
                     SNKIKKTATSDWMEIEKQRGISVATSVMGFEYRDYKINILDTPGHQDFAEDTFRTLTA
                     VDSVIIVIDTAKGVETQTRKLMQVCRMRNTPVMVFVNKMDREGKEPFDLLDEIESELG
                     IAVRPLSWPINMGVGFKGVYNIYKKSLDLYTPNKQVISESIAFNDLSNKELEKHIGEN
                     NAAKLREDIELIEGVYEPFDVNTYLNGGLAPVFFGSALNNFGVKELLDCFVEIAPSPR
                     AVEATERVVKPEESNFSGFIFKIHANMDPNHRSCIAFVKICSGKFERNVNYKHIRHNK
                     LMKFSSPTAFMAQKKETVDEAFAGDIIGLPDTGTFKIGDSLTAGEEIHFKGLPSFSPE
                     MFKYIENADPLKAKQLNKGIDQLMDEGVAQLFVNQYNGRKIIGTVGQLQFEVIQYRLL
                     HEYNAQCKWEPISLYKACWIESDNAEELEQFKKRKFQYMAKDKEGRDVFLADSGYVLQ
                     MAQSDFKNIRFHFTSEF"
     gene            1818..>2329
                     /locus_tag="PKN90_04905"
     CDS             1818..>2329
                     /locus_tag="PKN90_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNZ85651.1"
                     /translation="MQIGKYNTLRVVKHVDFGVYLDGGEFGEILLPIRYVPANCEVDD
                     RVDVFVYVDSEDRIIATTEKPFAEVGDFAALQVKSVTKFGAFLDWGLMKDLLVPFSEQ
                     KVYLEEGKLAVVYIYLDEKTNRIVGSAKLEKWVTKIPAVFEENDQVEALIAKKTDLGY
                     KAIINNMYLG"
BASE COUNT          742 a          404 c          502 g          681 t
ORIGIN      
        1 ggaaagctcc agctttccct attcaaacca aactaccgct acctcacgaa caacgctaaa
       61 cgctaaacga acaacgttcc ccgtctctcg tgtggctttt ctttattatc aactatctac
      121 tactttcaaa ctttataaac tttcaaactt tacaagttct tacaatttcg taagcggatt
      181 tttattatct ttgcacctca attttagcac gataacatga cttttcaaga agaaataaaa
      241 cgaagacgca cttttgctat catcagccat cccgatgctg gtaagactac tttaaccgaa
      301 aaactcttgc tttttggtgg agctattcat attgcgggag cggtaaaatc gaacaaaatc
      361 aaaaaaactg ccacgtctga ctggatggaa attgaaaaac aacggggtat ttcggtggca
      421 acatcggtaa tggggttcga atatcgagat tataaaatca atattttgga tacgcctggt
      481 caccaagatt ttgccgagga tacgtttcgt acacttactg cggtagatag cgtaattatt
      541 gtgattgata ctgccaaggg ggtagaaaca caaacgcgta aactgatgca agtttgtcgt
      601 atgcgtaata cgcctgtaat ggtatttgta aataaaatgg atagagaagg aaaagaacct
      661 ttcgatttgc ttgacgagat tgaaagtgaa ttgggtattg ctgttcgtcc tttgagttgg
      721 cccatcaata tgggggttgg ttttaaaggg gtgtacaata tttacaaaaa aagtttagac
      781 ctatatactc ccaacaagca agttatttct gagtcaattg cctttaacga cctttcgaac
      841 aaagagttag aaaaacacat tggagaaaat aatgccgcta aattgcgtga agatatcgag
      901 cttatcgaag gagtgtacga gccttttgat gtaaatacct atcttaatgg tggtttggca
      961 cccgtatttt ttggtagtgc tttgaataat tttggggtaa aagaattgtt ggattgtttt
     1021 gttgaaatag ccccatcgcc acgtgccgta gaggctactg agcgggttgt aaaacctgaa
     1081 gagagtaatt tctctgggtt tatttttaag attcatgcca atatggaccc caatcatcgt
     1141 agctgtattg cttttgtaaa aatatgttca ggcaagtttg agcgtaatgt caactacaaa
     1201 cacattcgtc ataataagtt gatgaaattt tcatcgccta ccgctttcat ggcacaaaag
     1261 aaagaaactg ttgatgaggc ttttgcaggc gatattatcg gtttgcccga tacaggtacg
     1321 tttaaaattg gcgacagcct aaccgcaggt gaagaaattc atttcaaagg attacccagt
     1381 ttctctcccg aaatgtttaa gtatatcgaa aatgctgacc cattaaaagc caaacagcta
     1441 aacaaaggaa tcgatcagtt aatggacgaa ggtgtggctc aattatttgt aaaccaatat
     1501 aatggcagaa aaattattgg tacagttggt caattacaat ttgaagtaat tcagtatcgt
     1561 ttgttgcacg agtacaatgc tcagtgtaaa tgggagccaa tcagtttgta caaagcttgt
     1621 tggatagaaa gtgataatgc cgaagaatta gagcaattta aaaaacgcaa gttccagtac
     1681 atggcaaaag acaaagaggg cagagatgtc tttttagccg attcgggtta tgtattgcaa
     1741 atggcacaaa gcgattttaa gaatatacgt ttccatttta cgtccgaatt ttaaaaaata
     1801 taacacatac aaataccatg caaataggaa aatacaatac cttacgtgta gtgaaacatg
     1861 ttgattttgg ggtttacctc gatggcggcg agtttggcga aattttgttg ccgatacggt
     1921 atgttcctgc caattgcgaa gtggacgacc gagtggatgt gtttgtgtat gtagattcgg
     1981 aagaccgcat tattgccacc accgaaaaac cttttgccga agttggcgat tttgctgctt
     2041 tgcaggtgaa aagcgtaacc aaatttgggg catttttaga ttgggggctg atgaaagact
     2101 tgttggtgcc atttagcgaa caaaaagtgt atttagaaga gggtaaattg gctgtggtgt
     2161 atatttattt agacgaaaaa accaaccgca tagtgggttc ggctaaactc gaaaaatggg
     2221 ttacaaaaat acctgccgta ttcgaagaaa acgaccaagt agaagccttg attgcgaaaa
     2281 aaaccgattt aggctacaaa gctatcatca acaatatgta tttgggctt
//
LOCUS       DAOPUR010000242         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_48839_length_2325_cov_3.289868, whole genome shotgun sequence.
ACCESSION   DAOPUR010000242 DAOPUR010000000
VERSION     DAOPUR010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48839_length_2325_cov_3.289868"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..996
                     /locus_tag="PKN90_04910"
     CDS             112..996
                     /locus_tag="PKN90_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ85652.1"
                     /translation="MNYHFLRLIVFSFVVSITNSALAVTLHDNIFRIEDPKNASSSLM
                     LLNENTEDDFADVDDFENFDEISFNAPYNSWSNTAVNPYNINLLHSKDTFRVDVSGYT
                     HPLDKVQRITSHFGPRRTRYHYGIDLKLNVGDTVRSSFDGMVRIAKIGRGYGYYVLVR
                     HFNGLETIYGHLSKILVDTEQLVKAGDPIGLGGNTGRSTGPHLHYEVRYLGNAINPES
                     LICFDNLTVKTPELLVSAETFRYKLDVDRMRNYTVRRGDTLSTIARRQGTTIRNLCRL
                     NNITTKTTLRVGRVLRIS"
     gene            1135..>2325
                     /gene="secA"
                     /locus_tag="PKN90_04915"
     CDS             1135..>2325
                     /gene="secA"
                     /locus_tag="PKN90_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965014.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNZ85653.1"
                     /translation="MGLNSILSKLFGSKSQRDLKEIMPFIEKIKAIYPQVEQLSNDEL
                     RNRLQGVRLEIQQTVSDERERIATLKQSIDATPIEHRDKLYNEIDAIEKAIITKLEEA
                     LDNALPEVFAIVKDTARRFKENETIEVEATDFDRELAVTHDFVDIVSNKAVYKNEWEA
                     GGNVIKWDMVHYDVQLIGGVVLHKGKIAEMTTGEGKTLVATLPVFLNALSGNGVHVVT
                     VNDYLSKRDSEWMGPIYMFHGLRVDCIDKHRPNSDDRRKAYLADITFGTNNEFGFDYL
                     RDNMASTPEDLVQRIHNYSIVDEVDSVLIDDARTPLIISGPIAKGEEQLYEQYCSKVE
                     RIVAEQKNLVTKTFVEAKRLMQSADAKEQEKGSLLLFRAFKGLPKNKALIKYLSEPGV
                     KASML"
BASE COUNT          776 a          354 c          474 g          721 t
ORIGIN      
        1 aaaatgctgc aattgaacta aatcgctttt ttaagccaga agaaatatat aattttccac
       61 cacatagttt agacttttta tatgccaatg acgaattgta aaaataaaaa aatgaattat
      121 cattttttac gtcttattgt atttagtttt gttgtttcaa tcacaaatag tgcgttggct
      181 gtaactttgc acgataatat atttcgcata gaagacccga agaatgcatc tagcagtctt
      241 atgttgctaa acgaaaatac agaagatgat tttgctgatg tggatgattt tgaaaatttt
      301 gatgaaatat cttttaatgc accttacaac agctggagca atacggcggt caatccgtat
      361 aatataaatt tattacatag caaagatact ttccgtgtag atgttagtgg gtatactcat
      421 ccacttgata aggtacaacg cataacatca cattttggtc cgcgtcgtac tcgttatcac
      481 tatggtattg atttaaaatt gaatgttgga gatactgtgc gttcttcatt tgatggaatg
      541 gtacgtatag ccaaaatagg tagagggtat ggatattacg ttctcgtgag gcattttaat
      601 ggactcgaaa ctatttatgg tcatttaagc aaaatattag ttgatacaga gcagttagta
      661 aaagcgggtg atcctattgg tttaggagga aatacaggaa gaagtacggg tcctcattta
      721 cattatgagg tacggtattt gggaaatgcg attaaccctg aatctttgat atgttttgat
      781 aatttaacag ttaaaacgcc agaattatta gttagtgcag aaacctttcg gtataagttg
      841 gacgtggatc gtatgagaaa ttatactgta agaagagggg acaccttatc taccattgcc
      901 agaaggcaag gtactactat acggaatttg tgtcgactga ataatatcac cactaaaact
      961 acattgcgag taggtcgcgt tttacgtatt tcataacaag aaatacggtc tgtttaagat
     1021 ttattatctt taattaatct taaacagttt ctatataagc aaattaatat tgttatcttt
     1081 gtgcctttgt atatctaaaa ggcaacattt tttcatttag caacatttac cattatggga
     1141 ttaaatagta ttttaagcaa gttatttggt tcaaagtcac agcgtgattt aaaagagata
     1201 atgccattta ttgaaaaaat aaaggcaatc tatcctcaag tagagcaact ttcaaacgac
     1261 gaattgcgta ataggctgca aggcgtacga cttgaaattc aacaaactgt ttcggatgag
     1321 cgtgaacgta tcgctacgtt aaaacaatct attgatgcta ctcctattga acatcgagat
     1381 aaattgtaca atgaaataga tgctatcgaa aaagccataa taactaaatt agaagaggct
     1441 cttgataacg ccttgcccga agtgtttgct attgtaaaag atactgcaag acgttttaaa
     1501 gaaaatgaaa caatagaggt ggaagccact gattttgacc gtgaacttgc agtaacacat
     1561 gattttgtgg atattgtgag taataaagcc gtttacaaaa atgaatggga agctggtgga
     1621 aatgtcatta aatgggatat ggtgcactat gatgtgcaac ttataggtgg cgttgtattg
     1681 cataaaggta aaatagccga gatgaccaca ggggagggta aaacattggt agctactttg
     1741 cccgtctttt taaatgcctt gagtggtaat ggtgtacatg ttgtaactgt aaacgactat
     1801 ttatctaaac gtgactccga atggatggga cccatttata tgtttcatgg attacgtgta
     1861 gattgtatcg ataaacatcg tcccaattca gatgacagac gaaaagcgta tttggctgat
     1921 attacctttg gtaccaacaa cgagtttggc ttcgattact tacgggataa tatggcatct
     1981 actcccgagg atttagtaca acgaatacat aactactcca ttgtagatga ggtggactct
     2041 gtactgattg atgatgctcg tacacctctt atcatttcag gtcctattgc aaaaggggaa
     2101 gagcaattat acgagcaata ctgctcaaaa gtagaacgta ttgtagctga gcaaaaaaac
     2161 ctagttacga aaacatttgt tgaagcaaaa cgattaatgc aatctgcaga tgcgaaagag
     2221 caagaaaaag gctctttgtt actattcaga gcttttaaag ggttgccaaa aaataaggca
     2281 ctgattaaat atttaagtga gccaggagtg aaagcatcta tgctg
//
LOCUS       DAOPUR010000243         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_48938_length_2322_cov_3.232466, whole genome shotgun sequence.
ACCESSION   DAOPUR010000243 DAOPUR010000000
VERSION     DAOPUR010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48938_length_2322_cov_3.232466"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PKN90_04920"
     CDS             <1..1157
                     /locus_tag="PKN90_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNZ85654.1"
                     /translation="CYCTKSSHFSFVLEEVLKNGVHVETSSAFDMNILENLAETGHLK
                     KDTFIICNGFKRPLYVENIQSFINQGFDNTIAILDNKFELDLLLDGMEKNFKVGIRIA
                     SEEEPKFDFYTSRLGIRYNDILPYYYEKIKDNPMVDLKMLHFFINTGIKDTAYYWSEL
                     SKAVELYCELKKICPELDSLDIGGGFPISTHLDFNYDYEYMAEEIVAQIKNICTQNNV
                     PEPDIFTEFGTFTVGESGAILYSIVNQKQQNDRESWNMIDSSFITTLPDTWAISQRYI
                     LLAINNWDKEYQRVHLGGLTCDSEDFYSAGHSNAIFLPKVDPTKGEQYIGLFHTGAYQ
                     ESLAGFGGIQHCLIPAPKHVVIDLDENGEYYTKLFAKEQSYKSMLKILGY"
     gene            1162..2004
                     /gene="speB"
                     /locus_tag="PKN90_04925"
     CDS             1162..2004
                     /gene="speB"
                     /locus_tag="PKN90_04925"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNZ85655.1"
                     /translation="MERFAGVPDQYAVYETAKMVVLPVPYDETSSWIKGSDKGPEALL
                     DASVALEWYDIETQTQVYKKGIFTALPITEKSSPELLCNAVQERVTTYLKDGKFPVTI
                     GGNHTVSIGAIRAAASQYADLTILQMDAHSDLRPSYEGSQLNHACVMYQASQLCPITQ
                     VGIRSMCEEELGLFKEDRIFYAHTIASKSDWQQKALQTLSKNVYLTIDLDVFDPSVLP
                     STGTPEPGGLRYYEVLHFLRSVFAQYNVVGFDVVELCPNPNERASDFLAARLMYQLMT
                     YKFL"
BASE COUNT          783 a          352 c          466 g          721 t
ORIGIN      
        1 attgttattg tacaaaaagt tcgcattttt catttgtatt agaagaggtg ttgaagaatg
       61 gggtacatgt agaaacatct tctgcttttg atatgaatat attggaaaat ttagcagaaa
      121 ctggtcatct caaaaaagat acctttatta tatgtaatgg atttaagcgt ccattgtatg
      181 tggaaaatat acaaagcttt atcaatcagg gttttgataa cactattgcc atattagata
      241 ataagtttga attagattta ttattggatg ggatggaaaa aaacttcaaa gtaggtattc
      301 gtattgcatc ggaagaggaa ccaaagtttg atttttatac ttctcgtttg ggaattcgct
      361 ataatgatat tttaccatat tattacgaga aaattaaaga taatccgatg gttgatttaa
      421 agatgttaca tttttttatc aacacaggaa taaaggatac tgcttactat tggagtgaat
      481 tgagcaaagc cgtagagttg tattgcgaat taaaaaagat atgcccagag ctcgacagtt
      541 tggatatagg tggtggattt ccaataagta ctcatcttga ttttaattac gattatgagt
      601 atatggcaga ggaaatagta gctcaaatca agaatatttg tacacagaat aatgtgcctg
      661 aaccagatat atttaccgaa tttggaacgt ttacagtagg tgaaagtggt gcaatcttat
      721 attctattgt caatcaaaag caacaaaatg atagagaatc atggaatatg attgatagtt
      781 cgttcattac tacattgcct gacacatggg caattagtca gcggtatata ttgttagcta
      841 tcaacaattg ggataaagaa taccaacgtg tacacttggg aggtttgacg tgcgatagtg
      901 aagattttta tagtgcggga cattcaaatg ctatattctt gcccaaagtt gaccctacca
      961 aaggtgaaca atacatagga ttgtttcata cgggagctta tcaggagtcg ttagctgggt
     1021 ttggtggcat acaacattgt ttgattcctg ctcctaaaca tgtggtaatt gacttagatg
     1081 aaaacggtga atactataca aaactttttg caaaagaaca gagttataaa tcgatgttga
     1141 aaattttagg atactaagaa gatggaaaga tttgccggag tacccgatca atatgctgtg
     1201 tatgaaacag caaaaatggt agttttacca gtaccgtatg acgaaactag ttcgtggata
     1261 aaaggctctg acaaaggacc tgaagccttg ttagatgcgt cggttgcatt agaatggtat
     1321 gatatagaaa cacaaactca ggtatacaaa aagggaattt ttaccgcctt gccaattact
     1381 gaaaaatcat cacccgagtt gctttgcaac gcagtacaag aacgcgtaac cacctattta
     1441 aaagatggaa aattccctgt tactattgga ggtaatcaca ctgtaagtat tggagctatt
     1501 cgagcggcag catcgcaata tgctgactta actattttac aaatggatgc tcattccgat
     1561 ttgcgtccaa gctacgaggg ttctcaactt aaccatgcct gtgtgatgta ccaagcatcg
     1621 caattgtgtc cgattacgca agtaggtatt cgtagtatgt gcgaagaaga gttaggactg
     1681 tttaaagagg acagaatttt ttatgcacat accattgcaa gcaaatcaga ttggcagcag
     1741 aaagcccttc aaacactcag taagaatgta tatcttacga tagatttaga tgtgtttgac
     1801 ccttctgtat tgccatcaac aggaactccc gaaccaggag gacttcgcta ttatgaagta
     1861 ttgcattttt tgcgaagtgt atttgctcaa tacaatgtag taggttttga tgtggtagaa
     1921 ttatgcccaa atccgaatga aagagcttct gattttttgg ctgcacgttt aatgtatcaa
     1981 ttgatgacgt ataagttttt ataagcgaaa tttttgtacc atttttagtg atattattgc
     2041 gggatattta ttggtttgta gaaactatat aaacgatttt cctaacaaaa atcttttgaa
     2101 taacaattat caccacatca gtttgtgttt gtgcatcgtt cgtcgtagct gtagaagcct
     2161 taaaatgagt atcaatggat acaaattagt aaaacaacaa cttgcactct aataagtaac
     2221 aattatgaaa cgaacatgtt tgcgaaaaaa cacaaacacc aatgaaaata gcaaacatgg
     2281 agttccatcc aaccttaaac taaaaattat ttacggatga aa
//
LOCUS       DAOPUR010000244         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_49006_length_2320_cov_2.727594, whole genome shotgun sequence.
ACCESSION   DAOPUR010000244 DAOPUR010000000
VERSION     DAOPUR010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49006_length_2320_cov_2.727594"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKN90_04930"
     CDS             complement(<1..351)
                     /locus_tag="PKN90_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85656.1"
                     /translation="MKQSILFLFVVFVCLLSSCSQTEEYTIQGTVSGVETDTLFLEKV
                     ELGKIEIVAYQVLKKNGKFSFSFPRAVEPHYVRLRLQQGVLYLVADSTETISVYSNKQ
                     TFGQADSIVGSAASK"
     gene            510..1532
                     /locus_tag="PKN90_04935"
     CDS             510..1532
                     /locus_tag="PKN90_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNZ85657.1"
                     /translation="MNTAFRLTRFSLFVLIVLSTMSCRNQQKQFFINEGLVYGTYYRI
                     TYEHYQDIEVDIKEAMAKVGTSLSTFDSLSVISRINRNDSTVVADTYFTEVFTTAQQV
                     STHTGGAFDITVAPLVNAWGFGFDPQHERTAILIDSIKQWVGYNTISLQNGKILKQHP
                     QTQLDASAIAKGYACDVVAQVLREAGIENFLVDIGGELVVKGHNKRNTDWQVGINKAI
                     DDATQQNQEIQQIISVTDVAIATSGNYRQFYYENNKKIAHTIDPRTGYPTNHSLLSTT
                     VIAPTCMLADAYATAFMVMGNWEEIMKVVNNAVPPMAVFCIFEDTTGKHIEMASPGFE
                     QYLQEK"
     gene            complement(1744..>2320)
                     /gene="fabG"
                     /locus_tag="PKN90_04940"
     CDS             complement(1744..>2320)
                     /gene="fabG"
                     /locus_tag="PKN90_04940"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNZ85658.1"
                     /translation="AKAYASNAANFDDTHNVVSEIVKEFGRIDILVNNAGITRDGLMM
                     RMTEQQWDMVINVNLKSAFNFVHAITPIMMKQKTGSIINMSSVVGVSGNAGQCNYSAS
                     KAGMIGLTKSIAKELGSRGVRANAIAPGFIITEMTAALSDEVRDQWAAQIPLRRGGTP
                     EDVANVATFLASDMSSYVSGQTIHCCGGMNM"
BASE COUNT          758 a          469 c          400 g          693 t
ORIGIN      
        1 tttagatgct gctgacccaa ctatactatc tgcttgacca aatgtttgtt tgtttgaata
       61 gacagaaatc gtttcggtag agtcggctac taaatacaat acaccttgtt gcaaacgcaa
      121 acgcacatag tgaggttcaa ctgcacgagg aaaggaaaaa gaaaacttgc catttttttt
      181 aagtacttga taagcgacta tctctatttt tcccaactca actttttcca aaaataaagt
      241 atctgtttca actcccgaca ctgtgccttg aatggtatat tcttccgttt gagagcatga
      301 tgacaataaa caaacaaaca ccacgaataa aaataagatt gattgcttca tactgctcat
      361 actggtataa cattacatgt accgaatacc ctataataat tgtgtaaata aaccttgcaa
      421 aaatacaaca tctttcagct aaaatcaaaa aaatcgtatc tttgtacgct taaaatgagt
      481 attttattgt ctattaacac atattgctaa tgaataccgc attccgatta acacgttttt
      541 ccctttttgt tcttattgta ctttctacaa tgagttgtag aaaccaacaa aaacaatttt
      601 ttataaacga aggtttagta tatggtacct attaccgcat cacctacgag cactatcaag
      661 atatagaagt tgacataaaa gaagctatgg cgaaagtagg aacatcttta tctacatttg
      721 acagcctttc tgtaatttct cggattaaca gaaatgatag caccgtagtg gctgacactt
      781 attttacgga agtatttacg acggctcaac aggtttcaac acatacggga ggtgcttttg
      841 acataacagt tgcaccattg gtaaacgcat ggggatttgg ttttgaccct caacatgaaa
      901 gaacagccat cctaatagat agcatcaaac aatgggttgg atacaacaca atatccttac
      961 aaaacggcaa aatcctgaaa caacacccac aaacgcaact tgacgccagt gctatagcca
     1021 aaggatatgc gtgcgatgta gtagctcagg tattacgtga ggctggtatc gaaaattttt
     1081 tggtggatat tggtggcgaa ttggtagtaa aaggacataa taagagaaac actgattggc
     1141 aagtaggtat caacaaggca attgatgatg ctacgcaaca aaaccaagaa atacaacaaa
     1201 ttatttcggt aacagatgta gccattgcta cttcgggcaa ttatcgtcag ttttactacg
     1261 aaaacaacaa aaaaattgcc cataccatcg acccccgtac tggctaccct accaatcatt
     1321 cgctgttgag caccaccgta attgctccta cttgcatgct ggctgatgcg tatgctactg
     1381 cttttatggt aatgggaaat tgggaagaaa taatgaaagt agtaaacaat gctgttcctc
     1441 ctatggctgt tttttgtatt tttgaagata ctacaggcaa acatatcgaa atggcttctc
     1501 ctggttttga acagtacttg caggaaaagt aatcggtaat ttatcacaaa ccaagcttac
     1561 ttacggataa aatttgtgta ttttatcacc acgtatttta tgtttaaaca aaaaaataac
     1621 tattttttta tccctctatg ccattattaa acaaatccat ccaataaaaa ttgcttcttc
     1681 tgatttcgaa aaatatctct atcaaaagaa gcaataaggt gttatcaaga aattggaaaa
     1741 atcctacata ttcataccac cacagcagtg aatagtttgc ccagatacat agctagacat
     1801 atccgaagcc aagaaagtag ctacattcgc cacatcttcg ggtgtaccgc ctctacgcaa
     1861 tggaatttgt gcagcccatt ggtctcttac ctcatccgaa agggctgctg tcatttcggt
     1921 aataataaat ccaggtgcaa tggcattggc tctaacacct cgcgagccca attctttggc
     1981 tatcgatttg gttaaaccaa tcatacctgc tttggatgca gaatagttac attgtccagc
     2041 attaccactt acgcctacta ctgacgacat gttgatgatt gaacctgttt tttgtttcat
     2101 cataataggt gtgatggcat gtacaaagtt gaaagctgat ttcaagttta cattgattac
     2161 catatcccat tgttgctcag tcatacgcat catcaaccca tcgcgggtga tacctgcatt
     2221 atttactaaa atatcaatac gtccaaattc ttttacaatt tcagatacca cattgtgtgt
     2281 atcgtcaaaa ttggcagcat tagacgcata cgctttggct
//
LOCUS       DAOPUR010000245         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_49086_length_2317_cov_2.992042, whole genome shotgun sequence.
ACCESSION   DAOPUR010000245 DAOPUR010000000
VERSION     DAOPUR010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49086_length_2317_cov_2.992042"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /gene="alr"
                     /locus_tag="PKN90_04945"
     CDS             <1..1303
                     /gene="alr"
                     /locus_tag="PKN90_04945"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNZ85659.1"
                     /translation="QKNISRFIGIGKHISQYAHLFEGKTYFYHTTKEFLFSNIIDSFH
                     NEAILVKGARSFEFEKIINKLELKMHETVLEVNLNALVNNFNYLRSYLLPSTKTMCMI
                     KANAYGCGAIEVAKTLQYHRCDYFGVAVADEGVELRKAGIHTPIIVMNPEPSSFDLLC
                     EYRLEPEIYSFKLLKAFAEHLNKYGIINYPIHIKIDTGMKRLGFEPTDMNTLIHFIKA
                     QTSCQVQSIFTHLVGADDAIFDDFTQHQLKLFTQCCDVLQSHCNHPIIRHALNSAGVE
                     RFPNFQFDMVRLGIGLYGIATSNQHAIQPLVTLKTIVLQVKNVAAHETVGYSRKGSLH
                     RNSRIATIPIGYADGLKRNLGNGNGYVLIDGKKAPFVGNICMDISMVDITDIAHVEEG
                     DTVVVIGNQIHINEIAEKAGTIPYEILTGVSQRVKRIYFQE"
     gene            1433..2014
                     /locus_tag="PKN90_04950"
     CDS             1433..2014
                     /locus_tag="PKN90_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85660.1"
                     /translation="MKKVWMYIVLSTVLFTGCIADKRNLQFVESLVETELSNDALQLR
                     YRAPKGFHLVDQHYTDSIANVQLLQDGFSPVVLSIHVDTLKGASMMLSDIRYLPEDKL
                     EKEIKNYKKTYNQHNTWNKVNRMTYSHHNFDIVEINCENDTYMLTKLFFYEDKHPTFV
                     VDYFIPIVEKETYTPLINSSIASFEKGYTLIFH"
     gene            complement(2019..>2317)
                     /locus_tag="PKN90_04955"
     CDS             complement(2019..>2317)
                     /locus_tag="PKN90_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNZ85661.1"
                     /translation="ALEAYFTVLIEIFWSKERIMEVYLNVIEMGNGIYGIEKASEIYF
                     NKPPKKLTSFQAALVAASLPNPRKYSITHPGPYMRKRQAHILWLMPKLGKIEFE"
BASE COUNT          816 a          397 c          401 g          703 t
ORIGIN      
        1 acaaaaaaat atttctcgtt ttattggcat tggaaaacac atatcccaat atgctcattt
       61 atttgaagga aaaacctatt tttatcacac aacaaaagag tttttatttt ccaatataat
      121 tgacagtttt cataacgaag ccattcttgt aaaaggagct cgatcttttg aatttgaaaa
      181 aatcatcaat aaattagaac ttaaaatgca cgaaacggtg ctagaagtta acctcaatgc
      241 actggtaaac aactttaatt atttacgctc ttacctatta cccagtacca aaacaatgtg
      301 tatgataaaa gccaatgcat atggttgtgg tgcaatagaa gtggctaaaa cactacaata
      361 ccaccgatgt gattattttg gggtagctgt agcagatgag ggtgttgaat tacgcaaagc
      421 gggtattcat actcctatta ttgtaatgaa tccagaacca agctcgttcg atttgctgtg
      481 tgaataccgt ttagaacccg aaatatatag ttttaaatta ctgaaagctt ttgccgaaca
      541 ccttaataaa tatggtatta tcaactaccc tattcatatc aaaatagata ctggcatgaa
      601 acgattgggt tttgagccta ccgatatgaa tacattaatt cattttatca aagcacaaac
      661 atcgtgtcag gtacagtcta ttttcacgca tttggtaggg gctgatgatg ctatatttga
      721 tgattttacg caacatcaac taaaactttt tacccaatgt tgtgatgtac tacaatccca
      781 ttgtaaccac cccattatac gacatgcact aaatagtgct ggagtagaaa gatttcctaa
      841 ctttcagttc gatatggttc gtttgggaat aggtttgtat ggaattgcaa catccaacca
      901 acatgctatt cagcctttag taaccctaaa aactatcgta ttgcaagtaa aaaacgtagc
      961 tgcacacgaa acagttggtt atagcagaaa aggtagcttg caccgaaact cccgtatcgc
     1021 aaccatccca attggatatg ctgatgggct aaaacgtaac ctaggaaatg gaaatggata
     1081 tgtactcatt gacggtaaaa aagctccttt tgtaggcaat atttgtatgg atatcagtat
     1141 ggtagatatt acagatatag cacatgtaga agagggagat acggttgtgg tgattggaaa
     1201 tcaaatacat atcaatgaaa tagcagaaaa agcaggcact attccttatg aaatattaac
     1261 aggtgtatct caacgagtaa aacgtatata ttttcaagag taataagatg aaaggatttg
     1321 ctgtgtaagc gttgtgcaca aaaagcacaa aagaaataac agaacaaaat tgcagagaat
     1381 gtagtataaa actctgtgtg agaaaaataa ctagataata actgaataac agatgaaaaa
     1441 agtgtggatg tatattgttt tgagtaccgt gttgtttaca ggttgcatag cagataaacg
     1501 caacctacaa tttgtggaat cgttggtaga aacagaatta tctaacgacg cattacagct
     1561 tcgctatcgt gctccaaagg gttttcattt agttgaccaa cattatacag attctattgc
     1621 caatgtacaa ttgctacaag atggtttttc tcccgttgtt ttgagtatac atgtagacac
     1681 tctaaaagga gcttctatga tgctttcgga tattcgatat ctacccgaag ataaattgga
     1741 aaaagaaatt aaaaactaca aaaaaacata caatcaacac aatacttgga ataaagtaaa
     1801 tcgcatgacg tatagccatc ataattttga tatagtagaa ataaactgcg aaaacgatac
     1861 ctacatgctc acaaaattat tcttttacga agataagcat ccgacttttg ttgttgatta
     1921 ttttattcca atcgtagaaa aagagacata taccccactt ataaattcat ccattgcatc
     1981 gtttgaaaaa ggatatacac ttatctttca ttaatgtgtt attcaaactc aatttttcct
     2041 aacttgggca ttaaccatag tatatgtgct tgtcttttac gcatataagg acctggatgt
     2101 gttatgctat acttacgtgg attaggcaac gatgctgcaa ccaatgcggc ttgaaaagaa
     2161 gttaatttct tagggggttt attaaaataa atttctgagg ctttttctat accgtaaata
     2221 ccatttccca tttcaatcac attgagatac acctccataa tacgctcttt ggaccaaaaa
     2281 atctcaatca aaaccgtaaa ataagcttcc aaagctt
//
LOCUS       DAOPUR010000246         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_49320_length_2309_cov_2.965839, whole genome shotgun sequence.
ACCESSION   DAOPUR010000246 DAOPUR010000000
VERSION     DAOPUR010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49320_length_2309_cov_2.965839"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1107
                     /gene="rfbB"
                     /locus_tag="PKN90_04960"
     CDS             49..1107
                     /gene="rfbB"
                     /locus_tag="PKN90_04960"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNZ85662.1"
                     /translation="MEKRTILITGGAGFIGSHVTRLFVNKYPNYTLYNLDALTYAGNL
                     ENLTDIANKPNYNFIKGDITDEIFIQELFQKYQFDGVLHLAAESHVDRSITDPLAFIK
                     TNVLGTANLLTAAKNLWKNNMEGKRFYHISTDEVYGSLGKEGFFTEETPYDPRSPYSA
                     SKASSDHFVRAYYHTYGLPVVISNCSNNYGANHFPEKLIPLAINNIKHSKPIPIYGKG
                     ENIRDWLWVNDHARAIDVIFHTAKIGETYNIGGHNEWTNIDLIKKLCEIMDTKLGRPQ
                     GTSAQLITYVSDRAGHDMRYAIDASKLERELGWTPSLQFDEGLEKTVDWYLANEEWLD
                     NVTSGNYQSYYEKQYTDR"
     gene            1170..>2309
                     /gene="pepT"
                     /locus_tag="PKN90_04965"
     CDS             1170..>2309
                     /gene="pepT"
                     /locus_tag="PKN90_04965"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNZ85663.1"
                     /translation="MKSITERFLSYIEIDTQANETSNSTPSSMGQVLFAEMLEKELVS
                     LGLDVIRTDKSYVYATLKSNLTEKKIPTIAFIAHMDTSPAMSGKNIRPNIVKAYDGKD
                     IILQKEGILSPQQFPELLHYIGQDIIVTDGTTLLGSDDKAGIAAIVSAIEKICEDNNF
                     KHGDILFAFTVDEEIGKGADHFDLNLCRADWAYTIDGGEIGELEYENFNAAMATITIL
                     GNSVHPGYAKNKMINSMLIAQEVISLLKQFGLPENTSGYEGFFHLDKIEGNVEKTILQ
                     YIIRDFDIQDFTNRKANLSTIVRQMNEKYPLACINVLIEDQYYNMKSTIEPIMHIVSL
                     AEQAMIEVGVTPKIKPIRGGTDGARLSYMGLPCPNIFAGGHNFHSK"
BASE COUNT          826 a          392 c          417 g          674 t
ORIGIN      
        1 tctttttatg taattttgta cccgttaaat cctaatacat ttttttaaat ggaaaaaaga
       61 acaattttga ttacaggagg tgctggtttt attgggtctc acgtaacacg cttatttgta
      121 aacaagtatc caaattacac actgtataat ttagatgctc ttacgtatgc tggtaatttg
      181 gaaaacttaa ctgacatagc caataaacca aattacaatt ttataaaagg ggatattacc
      241 gacgaaatat ttatccaaga attattccaa aaataccaat ttgatggtgt attgcacctt
      301 gctgctgaat ctcacgttga ccgttcaatt accgacccat tagcctttat taaaaccaat
      361 gtattgggta ctgctaatct attaacagct gctaaaaatt tgtggaaaaa caacatggag
      421 gggaaacgtt tttaccatat ttcaaccgat gaagtgtatg gctcactagg taaagaaggt
      481 tttttcacag aagaaactcc ttacgaccct cgcagcccct attctgcttc taaagctagt
      541 tctgaccact ttgttcgtgc ctattatcac acctatgggt tacctgtagt aatttccaat
      601 tgctcaaata actatggagc gaatcacttc ccagaaaaat taattccact agctatcaat
      661 aatataaaac atagcaaacc aattcccatt tacggaaaag gagaaaatat ccgtgattgg
      721 ttgtgggtaa acgatcatgc acgtgcgata gatgttattt ttcatactgc taaaattggt
      781 gaaacatata acattggtgg acacaatgag tggacaaaca ttgatttgat taaaaagctg
      841 tgcgaaatca tggatacaaa acttggtcgt ccacaaggaa catcagccca actaattacg
      901 tacgtttcag atagagctgg tcatgatatg cgttatgcta ttgatgcaag caaattagaa
      961 cgtgaattag gatggactcc ttctttgcag tttgatgaag gattagaaaa aacagtagat
     1021 tggtacttag caaacgaaga atggcttgat aatgtaacga gtggaaatta ccaaagctat
     1081 tacgaaaaac aatatacaga tagataacat acaaatttca atcatcaaag aggagtgtgt
     1141 gcacactcct ctttttagta tactacaaaa tgaaaagcat cactgaacgt tttttatctt
     1201 atattgagat tgatacacaa gctaatgaaa catcaaactc aacccctagt tccatgggtc
     1261 aagtactatt tgctgaaatg cttgaaaaag aattagtatc attgggatta gacgtaatac
     1321 gaacagataa atcatatgtg tatgctaccc tcaaaagtaa cttgaccgag aaaaaaattc
     1381 ctactatagc ttttattgca catatggata caagtccagc aatgagtggg aaaaacatac
     1441 gacccaacat tgtaaaagca tatgatggaa aagatatcat actacaaaaa gaaggaatct
     1501 tatcccctca acaatttcct gagctcctgc actatattgg acaagacata atagtaaccg
     1561 atggcaccac cttgctagga agcgatgata aagcaggtat tgcagctatt gttagtgcta
     1621 tagaaaaaat ttgcgaagac aataacttca aacatggaga tatattattc gcatttactg
     1681 ttgatgaaga aattggaaaa ggggcagatc attttgactt aaatttatgt cgagcagatt
     1741 gggcatacac aattgacggt ggggagattg gcgaattaga atatgaaaat tttaatgctg
     1801 caatggctac aattaccatt ctaggaaaca gcgtacatcc gggttatgcg aaaaataaaa
     1861 tgattaactc tatgctcatt gcacaagaag ttatttcatt gctcaaacaa tttggattac
     1921 ctgaaaacac aagtggttat gagggatttt tccatttaga taaaattgaa ggaaatgtgg
     1981 aaaaaactat tttacagtat attattcgtg attttgatat acaagatttt acaaatagaa
     2041 aagcaaattt atccactata gtacgacaaa tgaatgaaaa atatccctta gcttgcataa
     2101 acgttttaat tgaagatcag tattacaata tgaaatccac tattgaacct attatgcata
     2161 tagtaagctt agcggaacaa gcaatgatag aagtaggtgt aacaccaaaa ataaaaccaa
     2221 tacgtggagg gacggatggt gctcgtcttt cgtacatggg gctaccctgt cccaacattt
     2281 ttgctggcgg acacaatttt catagtaaa
//
LOCUS       DAOPUR010000247         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_49499_length_2303_cov_2.822509, whole genome shotgun sequence.
ACCESSION   DAOPUR010000247 DAOPUR010000000
VERSION     DAOPUR010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49499_length_2303_cov_2.822509"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..688
                     /locus_tag="PKN90_04970"
     CDS             158..688
                     /locus_tag="PKN90_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85664.1"
                     /translation="MAEEKALASGANVLIINPTFMIGAYDSKPSSGQIITMNYGKRIV
                     FAPKGGKNFVAASDVANAIVNALRSTMIGEKILTGGYNLSFADFYKTMAQTQGYKISV
                     ITLSNSLLYAVGIIGSLCRKFGIKTPVSFTNMRILCTREWYTAEKAIHALQMPQTSIE
                     KAITDCINWFKANNML"
     gene            694..1614
                     /gene="queG"
                     /locus_tag="PKN90_04975"
     CDS             694..1614
                     /gene="queG"
                     /locus_tag="PKN90_04975"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNZ85665.1"
                     /translation="MNKQQLTDTIKQQALTLGFDAVGIAPSQLLEQDKEYLIKWLNKG
                     YAASMKYMHTHLEKRVNPASLLENTRSVIVVLYHYLPTTQQASQQYKIAKYAYTPDYH
                     YIIKSKLQQLASLLPTENADIQRWFTDSAPVLERRWAQLAGLGWIGKNGCFIHPQLGS
                     YVFIGEIFTEHELVYDTPYTHDHCGTCTRCLQACPTQALIAPRELDANKCISYHTIES
                     KNEIPSSIAQQLKGYIFGCDICQDICPWNAKIVAKEKKDSPTMPYVYYSDQDWLTLDK
                     KKFKQDFRHSPMQRAGYVKLIKNIRTVVEK"
     gene            1775..>2303
                     /locus_tag="PKN90_04980"
     CDS             1775..>2303
                     /locus_tag="PKN90_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ85666.1"
                     /translation="MGYLFSSESVSEGHPDKVADQISDALLDQFLAQDASSKVACETL
                     VTTGQIILAGEVKSEAYVDLQKTARNVVKRIGYTKSEYMFDADSCGVLSAIHEQSPDI
                     NRGVESTNPDDQGAGDQGLMFGYACNETKNLMPIALDLSHALLKELAAIRKEGKVMTY
                     LRPDAKSQVTIEYGDD"
BASE COUNT          795 a          438 c          427 g          643 t
ORIGIN      
        1 ctatttccgt aagatttatg aatagcaatt aaatcgaaag gctttagttt taaaacagct
       61 tactcagggc tgtttttgtt tgaaagaata tcaactgttt aaagttgtat ttaataatgc
      121 ggataattaa gataatttat atgccaaaag taaactgttg gcagaagaaa aagccttagc
      181 aagtggtgca aatgtcctca tcatcaatcc tacttttatg attggtgcgt atgatagcaa
      241 gccaagttca ggacaaataa ttaccatgaa ttatggtaaa cggattgtgt ttgcccccaa
      301 aggaggtaaa aattttgtgg cagcaagcga tgtggcaaat gccattgtaa atgcactccg
      361 ttcaactatg ataggagaaa aaattcttac tggtggatac aatctttctt ttgccgattt
      421 ttataaaaca atggctcaaa cacaaggcta caaaataagc gttatcacct tatccaacag
      481 tctgttatat gctgtaggta tcattggttc actttgtaga aaattcggaa ttaaaacacc
      541 cgtttcgttc accaatatgc gtatattgtg tacaagagaa tggtacactg cagaaaaagc
      601 catacacgca ctacaaatgc cacaaacgtc tattgaaaaa gcaattaccg attgcatcaa
      661 ttggtttaaa gcaaataata tgttgtaatg gttatgaaca agcaacaact cactgacacc
      721 atcaaacaac aggctcttac attgggcttt gatgcggtgg gaattgctcc ttctcaatta
      781 ttagagcaag acaaagagta tctcatcaag tggctaaaca aaggctatgc cgcctcaatg
      841 aagtacatgc atacacatct tgaaaaaaga gtaaacccag cctcattgct cgaaaataca
      901 cgttcggtga ttgtagtttt gtatcactat ttacctacta cccagcaagc cagtcagcaa
      961 tacaaaatag caaaatatgc ttatactcct gattatcatt atattatcaa atctaagcta
     1021 cagcaattag catcactatt accaacagaa aatgctgata ttcaacgttg gtttaccgac
     1081 tcggctcccg tattagaaag acgttgggca caactagctg gactgggttg gattggtaaa
     1141 aatgggtgtt ttattcatcc tcaattaggc tcgtatgtgt ttattggaga aatatttacc
     1201 gaacacgaat tggtgtacga cacaccctac acgcacgacc attgtggcac atgtacacgt
     1261 tgtttacagg cttgtcccac ccaagcctta atagctccac gagaactaga tgccaataaa
     1321 tgtatttcgt accacacgat agaaagtaaa aatgaaattc cttcctctat cgcccaacaa
     1381 ctcaagggtt atattttcgg gtgtgatatt tgccaagata tatgcccatg gaatgctaaa
     1441 attgtggcga aagaaaaaaa ggattctccc acaatgccct atgtgtatta tagcgaccaa
     1501 gattggttaa cgctagataa aaagaagttt aaacaggatt tccgacactc gcctatgcaa
     1561 agagcgggat atgttaaact aataaaaaac atacgaacag tagttgaaaa ataaaaaata
     1621 acttctacct ttgcaccatc aaagtggatg gatttggata gcttgcaacc acagaaaaaa
     1681 atgctaaaac aaaaaacata cttttctgcc tttttcgata tccaaaactc aaatcacttt
     1741 tattatttta taacaaacta ataattaaag tattatgggg tatttatttt catcagaatc
     1801 agtttctgaa gggcatccag acaaggttgc cgaccaaatt tccgacgcat tactcgatca
     1861 atttttagca caagatgcgt cttcaaaagt agcatgcgaa accttagtta caactggaca
     1921 aattattttg gctggtgaag taaaatcaga agcgtatgta gatttacaaa aaacagcacg
     1981 caatgtggtt aaacgtattg gctacaccaa aagcgaatac atgtttgatg ccgattcgtg
     2041 tggtgtatta agtgctattc acgaacaatc gcctgatatc aatagaggcg tagaaagtac
     2101 caatcctgac gatcaaggtg caggagacca aggattaatg tttggttatg cctgcaacga
     2161 aacaaaaaac ctgatgccga tagcattgga tttatctcac gccttgctca aagaattagc
     2221 agctatacgc aaagaaggta aagtaatgac atacttgcgt ccagatgcta aatcgcaagt
     2281 tactattgaa tacggtgacg acc
//
LOCUS       DAOPUR010000248         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_49542_length_2302_cov_2.406765, whole genome shotgun sequence.
ACCESSION   DAOPUR010000248 DAOPUR010000000
VERSION     DAOPUR010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49542_length_2302_cov_2.406765"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PKN90_04985"
     CDS             <1..825
                     /locus_tag="PKN90_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNZ85667.1"
                     /translation="ISFNYNKVLGLSENQTAMLTNGYFDQNFTSPNYIAKLHYPMGMM
                     YGYIYEGTYKLDEFDFDGTNYTLKSGIPHYTAENNTQPGYPKYADLNGDGVVDSNDQT
                     FIGRGDPLHIGGFSNTFIYKNFDLSIFFQWSYGNDILNANRLIFETGYNKRKDLNQYA
                     SFINRWTFENPNSNIPRVSTSSSNLLFSTRIIEDGSYLRLKTLSIGYSISAKLLNKVK
                     INKARISLSGQNLFTITNYSGYDPEVSIRNSALTPGLDFSSYPRAKSVNMGVNLSF"
     gene            845..>2302
                     /locus_tag="PKN90_04990"
     CDS             845..>2302
                     /locus_tag="PKN90_04990"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNZ85668.1"
                     /translation="MKKIKIISILSVLVFLSVSCSDFLDKEPYTLTPETYFNNETELE
                     TFLTGVYSPLMQEFFYGNYYPLYNAGGDDLTFYQRSSPATSILCANANSSNTYLNTFW
                     KILYEGINRANILLENVDNNTNISQATRDRIRGEALFLRAFYYFHLVEGWGDVPFRLK
                     STKSVYGLDAERTPKQIIYDQIIKDITTAIPYLYTSANIPYTGRVTQSAAKGILARIY
                     LFRAGENNRDSEVGNPLTNTSDSILAYYRHAKQWALEVKESGLHGLVNPYKQVFIDMC
                     QDKYNSTGIRESIWEVEEAGNRSTTEQAAGRIGNTLGFGSTNDYSTVPAYQNLTGMAN
                     PGYSYKFAYASLKLYEMYESEGDTARGDWSIANYEYTYDTNAPKAVTGRKYYYGKLPA
                     GLTSVEGYPCTEEAASTSNKNKTRCAAKYRRELEVVTPKNKNYTPINFPILRYSDILL
                     MIAEADNEINASPTELAYDCLDEVRNRAHITPLKGS"
BASE COUNT          772 a          424 c          413 g          693 t
ORIGIN      
        1 atttcattta attacaacaa agtattggga ctttctgaaa accaaacagc aatgttaaca
       61 aatggttatt ttgaccaaaa ctttacatct ccaaattata tcgccaaatt acattatcct
      121 atgggtatga tgtatggtta tatttatgaa ggcacataca aattggatga gtttgacttt
      181 gatggcacca attataccct caaatctgga atacctcatt acacggcaga aaataataca
      241 cagcccggct atccaaaata tgcggatttg aatggtgatg gagttgttga ttcgaacgac
      301 caaactttta taggacgagg agacccatta catattggag gcttttcaaa tacttttata
      361 tataaaaact tcgatttaag tatctttttc caatggtctt atggtaacga tattttaaat
      421 gccaatcgat tgatattcga aacgggttat aacaaacgta aggatcttaa ccagtatgcg
      481 tcctttatta atcgttggac ttttgaaaac cctaatagta acataccaag agtaagcact
      541 tcatcttcca atcttttatt ttcgaccaga ataatcgaag atggttctta cctacggtta
      601 aagacattat cgataggata ctctatcagt gcaaaattac taaataaagt aaaaatcaac
      661 aaggcaagaa tttctttatc aggtcaaaac ctgtttacta taaccaatta ctctggctac
      721 gatcctgaag tatctattag aaattctgct ttaactccag gacttgattt ttcatcgtat
      781 cctcgtgcaa agtctgtaaa tatgggtgta aatttgagtt tttaatccta taaaatgaaa
      841 agcaatgaaa aaaataaaaa taatttcaat attaagtgta cttgtctttt taagtgtttc
      901 ctgctctgat tttctggata aagaaccgta cacactgacg cctgaaactt acttcaacaa
      961 tgaaacagaa cttgaaactt tccttacagg agtttacagt ccactaatgc aagaattttt
     1021 ctatggtaac tattaccctc tttacaatgc aggcggtgat gacttgactt tttatcaacg
     1081 ttcgagtcct gccaccagta tattatgtgc caatgcaaat agtagcaaca catatctcaa
     1141 cacgttttgg aaaatattgt acgaaggaat aaaccgtgcc aacattttgc tggaaaacgt
     1201 agataataac accaatattt cgcaagccac acgtgataga atacgcggtg aagcactttt
     1261 cttgcgagct ttttattatt tccatttagt agaaggttgg ggcgacgttc cttttagatt
     1321 aaaatctaca aaatcggttt atggtttgga tgctgagcgt actccaaaac aaattatcta
     1381 cgatcaaatt atcaaagata ttacaacagc tattccctat ctttatactt cagctaacat
     1441 tccctataca gggcgagtta cacagtcggc tgctaaagga atattggcac gtatatatct
     1501 tttccgtgct ggcgaaaaca atcgtgacag tgaagtaggt aatccgctta caaatacttc
     1561 cgattccatt ttagcttact atcgccacgc caaacaatgg gcacttgaag ttaaggaaag
     1621 tgggcttcat ggtttggtaa atccctataa acaagtattt atagacatgt gtcaagataa
     1681 atacaacagt actggtatcc gtgaaagtat ttgggaagta gaagaggcag gcaatcgcag
     1741 taccactgaa caagctgcag gacgaattgg gaatacactc ggttttggta gtacgaatga
     1801 ttattcaacg gtaccagctt atcaaaatct tactggaatg gctaatccag gttatagtta
     1861 taaatttgct tatgcgtcac taaaacttta cgaaatgtat gagtcagaag gtgatacagc
     1921 acgtggtgat tggagtattg ctaattatga atacacgtat gataccaatg caccaaaagc
     1981 tgttacgggt cgtaaatatt attatggtaa attgcctgct ggactaacca gcgtcgaagg
     2041 ttacccttgt acagaagaag cagcttcaac cagcaacaag aacaaaacgc gttgtgcagc
     2101 taaatatcgc agggaattag aagtggtaac accaaaaaat aaaaattata cccctataaa
     2161 tttcccgata ctccgctata gcgatatatt gttgatgata gctgaagctg ataatgaaat
     2221 caacgcctct cccacagagt tagcttatga ttgtttggat gaagtaagaa atcgtgctca
     2281 tattacaccc cttaaaggaa gc
//
LOCUS       DAOPUR010000249         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_49570_length_2301_cov_2.488869, whole genome shotgun sequence.
ACCESSION   DAOPUR010000249 DAOPUR010000000
VERSION     DAOPUR010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49570_length_2301_cov_2.488869"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKN90_04995"
     CDS             complement(<1..307)
                     /locus_tag="PKN90_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85669.1"
                     /translation="MKFVIYVIIFFYVMLTNVYAISEVSEIVAAKDSLHSDTATVMPS
                     CPLIKESWFTIPMGYYQQETSVALGIVGGYYFPSTNLRKISSISGSVIYTFRNQFIIN
                     "
     gene            complement(405..962)
                     /gene="frr"
                     /locus_tag="PKN90_05000"
     CDS             complement(405..962)
                     /gene="frr"
                     /locus_tag="PKN90_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNZ85670.1"
                     /translation="METKQYLTAAIEKMETTLLFLDDALAHIRAGKANVRILDAVRVD
                     YYGSMVPLSNVATLTTPDARTIAIQPWEKGLIKEIEKAILHSDVGITPENNGEMIRLG
                     IPPLTEERRKMLVKQSKQEGEEAKISVRNVRRDVIDAIKKAVKDGLAEDMAKDAEAEI
                     QKIHDKFIKKIDDAVFAKEKEIMTI"
     gene            complement(984..1691)
                     /gene="pyrH"
                     /locus_tag="PKN90_05005"
     CDS             complement(984..1691)
                     /gene="pyrH"
                     /locus_tag="PKN90_05005"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNZ85671.1"
                     /translation="MVYKRILLKLSGESLMANKPYGIDENRLQDYAFQIQEIVNLGVE
                     VAIVIGGGNIFRGMSGVAKGYDRVKGDQMGMLATVINSLALASALESINVVSHVLTAI
                     RMEPIGEYYSKQKAIDYLKKGEVVIVSAGTGNPFFTTDTASALRAIEMETDVMLKGTR
                     VDGIYTADPEKDVNAVKFDEISYDEIYNRNLKVMDLTATTMCKENKMPIYVFDMDTVG
                     NLKKVLLGEKIGTLVYV"
     gene            complement(1794..>2301)
                     /gene="rplQ"
                     /locus_tag="PKN90_05010"
     CDS             complement(1794..>2301)
                     /gene="rplQ"
                     /locus_tag="PKN90_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNZ85672.1"
                     /translation="HNKKFNHLGRKTSHRDAMLSNMAVSLIKHKRISTTLAKAKELRK
                     FVEPMITKSKEDTTHSRRNVFAQLQDKNAVTILFAEVSQKVADRPGGYTRILKTGFRL
                     GDAAQMCIIELVDYNENMLKDKVAKKSSKTRRAGKKTKAVAEKVATVKAPKEEVVTEQ
                     ETPTETVE"
BASE COUNT          679 a          489 c          295 g          838 t
ORIGIN      
        1 catttataat aaattgattt ctaaatgtgt atatcacact gccagatata ctactaattt
       61 tacgcaagtt tgtactagga aaataatatc caccaacgat acccaatgca acactcgttt
      121 cttgctgata atagcccata ggaatagtaa accaactttc tttaataagc ggacaagagg
      181 gcataacagt agctgtatca ctatgcaatg aatcttttgc agcaactatc tcagatactt
      241 ctgaaatagc ataaacattg gtaagcatca cataaaaaaa aataattaca taaataacaa
      301 acttcattaa agaccttctt taggtgttga tttacgttat tctggtataa aaaaccattc
      361 ctcgttgtta caaagaatgg taaaatactc gtttatttta aatattatat tgtcataatc
      421 tctttctctt tagcgaatac agcatcatca atctttttta taaacttatc atgaatcttt
      481 tgtatctcag cttctgcatc tttagccata tcctctgcta atccatcttt tacagctttt
      541 tttatcgcat caatcacatc tctacgaaca ttacggacac taatctttgc ctcttcgcct
      601 tcttgtttcg actgttttac taacatttta cgtctttcct ctgtcaaagg aggaatgcct
      661 aaacgaatca tttctccatt attttcagga gtaataccta catctgaatg caaaattgct
      721 ttctcaatct cttttattaa ccctttctcc catggttgta ttgcaatagt tcttgcatcg
      781 ggggtggtta atgttgcaac attactcaat ggtaccattg aaccataata atcgactctt
      841 acagcgtcta aaatacggac atttgctttg cctgcacgta tatgtgccaa agcatcatct
      901 aaaaacagaa gggtggtttc catcttctct atcgctgccg ttaagtattg ttttgtttcc
      961 atctttaagt tatatttacg tcgttataca tatactaatg ttccaatttt ttctcctaaa
     1021 agcacctttt ttaagtttcc aacagtatcc atatcaaaca cataaattgg cattttattc
     1081 tccttgcaca tcgtagtggc agttaaatcc ataactttaa ggttgcgatt ataaatctca
     1141 tcatacgaaa tttcatcaaa tttaacagca tttacgtctt tctcaggatc tgcagtatat
     1201 ataccatcta cccttgttcc ttttaacatt acatctgtct ccatttctat tgcacgcaat
     1261 gccgaggcag tatcagtagt aaaaaaggga tttcctgtac cagcagaaac aattaccacc
     1321 tctccttttt ttaaataatc tattgccttt tgtttgctat aatattcccc aataggctcc
     1381 attctaatag cagtaagaac atgagataca acatttattg attctaatgc agaagctaac
     1441 gctaaactgt taataacggt agccagcatc cccatttggt ctccttttac tctatcataa
     1501 cctttcgcaa cgccactcat tcctctaaaa atatttccac ctccaattac aatggcaact
     1561 tccactccta gattaacaat ctcttgaatc tgaaaagcat aatcttgtaa tcgattttca
     1621 tctatcccat acggcttatt cgccatgagc gattcgccac ttaattttaa taatatacgt
     1681 ttatacacca ttgcctaaaa attttacgaa caaagataca ttattttttt atactaaaaa
     1741 aaggaacaag cataatactc attcctttcc ttattctatc ctaattcaga agtctattct
     1801 accgtttctg ttggcgtttc ttgttctgta acaacctctt cttttggagc ttttacagtt
     1861 gctacttttt ctgcaacagc ttttgtcttt ttacctgctc tacgcgtttt agaagatttc
     1921 ttggcaactt tatctttcaa catattttcg ttgtaatcaa ctaattcaat aatacacatt
     1981 tgagctgcgt ctcctaatcg aaaacctgtc tttagtatac gagtatatcc acctggtcta
     2041 tctgctactt tttgagaaac ctctgcaaat aaaatcgtta cagcattttt atcttgtaat
     2101 tgggcaaaca cattccttct tgagtgcgtt gtatcttctt ttgatttagt aatcatgggt
     2161 tcaacaaatt tacgcaattc ctttgctttt gccaatgtcg tactaatgcg tttatgtttg
     2221 attagcgaaa ctgccatgtt agacaacata gcatctctat gtgacgtttt tctacctaaa
     2281 tgattaaatt tcttattatg t
//
LOCUS       DAOPUR010000250         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_49642_length_2298_cov_3.685243, whole genome shotgun sequence.
ACCESSION   DAOPUR010000250 DAOPUR010000000
VERSION     DAOPUR010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49642_length_2298_cov_3.685243"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PKN90_05015"
     CDS             complement(<1..972)
                     /locus_tag="PKN90_05015"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNZ85673.1"
                     /translation="MENIKLVIDNTLGFISKDKVFAYEKEVSACNSMLHNGTGKGNDF
                     LGWLSLPSSTSAELINDIKQTADLLRKECEVVVVAGIGGSFLGAKAVIDALSNTFIWL
                     KKDTNTPVIVYAGNNIGEDYLAELMDYLSDKKFGIINISKSGTTTETALAFRLLKTQL
                     EKQVGKAKAKNLIVAVTDASKGALRTLATQEGYKTYVIPDNVGGRFSVLSPVGLLPIA
                     VAGLDLNQLLAGARKMEEVCGLNTPFAENPAAIYAATRNELYRNGKKIEILANYQPKL
                     HFFAEWWKQLFGESEGKENKGIFNAAVDFSTDLHSMGQWIQEGERTIF"
     gene            complement(975..1970)
                     /locus_tag="PKN90_05020"
     CDS             complement(975..1970)
                     /locus_tag="PKN90_05020"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNZ85674.1"
                     /translation="MKAKRICIMGGGSWATALAKITMSKGDKINWYMRRPEQIEIFQR
                     LGNNPSYLSSIRFDTKKIKFYADINLAVKNSDILVFATPSPFLKQHLQKLTRTLSNKI
                     IVSAIKGIVPDENMIINDYFQKFYGVKQENIAIIAGPCHAEEVALERLSYLTIACANN
                     ELGTYLAERLSCSFLKTSITSDINGIEYSSVLKNVYAIAVGICHGLKYGDNFQAVLIS
                     NSIREINRFVDAVERNNERNTQDSAYLGDLIVTAHSRFSRNRTFGTMIGKGYSVKTSQ
                     LEMEMIAEGYYGTKCIKEINDTLNVDMPILDAVYNILYERIAPTIEIKLLTEKIR"
     gene            complement(1984..>2298)
                     /locus_tag="PKN90_05025"
     CDS             complement(1984..>2298)
                     /locus_tag="PKN90_05025"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNZ85675.1"
                     /translation="GIGMDRLVMAMTNQTSIQEVLLFPQMRPEKIAKKDTPARFIELG
                     MSEEWAQAIVKHSSLSIKDIASNIDAKLHQQLCGINKKHKLSLPNPTLADVQVWIKKA
                     SE"
BASE COUNT          694 a          413 c          343 g          848 t
ORIGIN      
        1 aaaaatagta cgttcgcctt cctgtatcca ttgtcccatc gagtgtaggt cggtagaaaa
       61 atcaacagca gcattgaata tccctttatt ttcttttcct tcgctttcac caaataattg
      121 tttccaccat tcagcaaaga aatgcagttt tggttgataa ttagctagaa tttcaatttt
      181 tttaccgttt ctatataatt cattacgtgt agcagcatat attgcagcag gattctcagc
      241 aaaaggagta tttaatccgc acacttcttc catttttcta gctccagcta acaattgatt
      301 taaatctaat ccagctacag caataggtaa aagtcctaca ggagaaagta ctgaaaaacg
      361 accaccaaca ttatctggaa ttacatacgt tttgtaacct tcttgtgttg ccaatgtacg
      421 caatgcacct tttgatgcat cagttacagc cactattaag tttttagctt tagctttgcc
      481 aacctgtttt tctaattgtg tttttaataa acgaaaagcc aaagcagttt cggtagttgt
      541 accagatttt gaaatattaa tgataccaaa ttttttatct gataaataat ccattaattc
      601 agctaaataa tcttcgccaa tattattacc ggcatataca attacgggtg tatttgtgtc
      661 ttttttcaac caaataaatg tgttagataa agcatcaatc acagcttttg cacctaagaa
      721 gcttcctcca atacctgcta caactactac ttcacattct ttacgtaata aatcagcagt
      781 ttgtttgata tcgttaatta attcagcaga agtgcttgat ggaagggata accatcccaa
      841 aaaatcattt ccttttcctg taccattgtg tagcatacta ttgcatgcac ttacttcttt
      901 ttcgtaagca aatactttat cttttgatat aaaacctaag gtattgtcaa ttaccaattt
      961 aatgttttcc atttttatcg tattttttct gttaataatt taatttcgat tgttggggct
     1021 atgcgttcgt acaatatatt atatactgca tcaagaatag gcatatctac atttagagta
     1081 tcattaattt ctttaataca tttagttcca taataacctt ctgcaatcat ttccatttct
     1141 aattgtgaag tttttacaga atatcctttt cctatcatgg tgccaaaagt acgattacga
     1201 ctaaaacgag aatgtgctgt tactattaaa tcgcctaaat aggctgaatc ttgcgtatta
     1261 cgctcattat tccgttctac tgcatctaca aaacgattta tttcacgaat actatttgaa
     1321 attagtactg cctgaaagtt atcaccatat tttagtccat gacatattcc tactgcaata
     1381 gcataaacat ttttgagaac agatgaatat tcaatgccat taatatcgct tgtaatagat
     1441 gtttttaaga atgaacagct taatctttcg gctaaatatg ttcctaattc gttattagca
     1501 catgcaatgg ttaagtatga aagacgctct agggcaactt cttctgcatg acaaggtcca
     1561 gcaatgatag caatattttc ttgtttaaca ccatagaact tttgaaaata atcattaata
     1621 atcatatttt catcaggaac tattcctttg attgcagata cgattatttt attacttaat
     1681 gtacgtgtta gtttttgtaa atgttgttta agaaaaggag agggagttgc aaaaactaaa
     1741 atatcagagt ttttaactgc taagttaatg tctgcataaa attttatttt ttttgtatca
     1801 aatctaatcg aactgagata ggaaggatta tttcctaagc gttggaatat ttcaatttgc
     1861 tcaggtcttc tcatgtacca attaatttta tctccttttg acatggtaat tttggctaaa
     1921 gcggttgccc agcttccacc tcccataatg catattcgtt ttgctttcat ttgttccata
     1981 ctgttattcg cttgcttttt ttatccagac ttgtacgtct gctaaagtag gattaggtaa
     2041 ggatagtttg tgttttttat tgataccaca cagttgctga tgcaattttg catcaatatt
     2101 gcttgcaata tctttgatag aaagagacga atgttttact atagcttgtg cccattcttc
     2161 agacatgcca agttctataa atcgtgcagg ggtatctttt ttagcaatct tttcaggacg
     2221 catttgtgga aataataata cttcttgtat actggtttga ttggtcatag ccataactaa
     2281 tcgatccatt cctatacc
//
LOCUS       DAOPUR010000251         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_50115_length_2284_cov_2.746523, whole genome shotgun sequence.
ACCESSION   DAOPUR010000251 DAOPUR010000000
VERSION     DAOPUR010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50115_length_2284_cov_2.746523"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1451)
                     /locus_tag="PKN90_05030"
     CDS             complement(249..1451)
                     /locus_tag="PKN90_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNZ85676.1"
                     /translation="MKKILSILFIFASVVLPALSQTVAELEERRKKALKELEITSSLI
                     SKTAEQKKTSLSQINILRVEINARQSLINVMNQELRNIDRNIGQLRKEAQEKQAEMDK
                     LKGEYAKLMYHSYYKKTKYESLMFILSAKSFAESYRRYRYVQQYAEFAQNKAQEIEKV
                     KADLENKLAEIEKARLERVAVLNDRRKENAKLQTEQRKQSNFVSQLSRKEKDLRRELK
                     KQQDQANAINRRIDQIIAEEARKSQKAQAAGTQEFALTKEQKLLSGGFEKNQGRLPWP
                     VERGVIVGRFGVQAHAVLKYVTTDNKGIYIQTAPNSDARAVYEGEVTQRFSIPGSNYT
                     VIVRHGNYRTVYSNLTDIYVKVGDNIKARQAIGRIFVDNEDDNKSVLYFQVWKERDIQ
                     NPENWISR"
     gene            complement(1457..2257)
                     /locus_tag="PKN90_05035"
     CDS             complement(1457..2257)
                     /locus_tag="PKN90_05035"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNZ85677.1"
                     /translation="MSNKSGIRLLLACMLLILVACKTTKIDTAQSVVPIQVPLKKIVA
                     NQQQIQTINASKVSFSIPMQNQQTPLRGSIKARIDSCMQISIQALFGIEVARIHLTQD
                     SLILLDRINQQYVAESFSSFGSTFPVSFRMLQAIILNRLVDVTTKDDFSDFLYQKDEN
                     LHIVSYENSNFAIQYLVNDALHLLRTTVRDAEKKHYMMAEYQQFDTYNTIVYPKKNAI
                     VMLFSQQQYAIDYSIEKVEFNQQIDLSIVIPPRYKQILLADIAKEYFN"
BASE COUNT          637 a          436 c          378 g          833 t
ORIGIN      
        1 gctatgctaa aaaccgaccg tgccaaaaac aaactttaaa atgcgtgata ttcatctttg
       61 tatagtttta ctcatctgct atcagagttg cacctatcca tttgttaatt tatctatcta
      121 aacgatctac tagattattt atttgattca atcctcatct ctgtagcaag tctgcaagct
      181 gtatctgtaa ttgtctttat aatcaacttc gatttaaagc attaaccacc ctcaaacaat
      241 cccctttttt acctactaat ccagttttcg gggttttgga tatcgcgttc tttccatact
      301 tgaaaataga gtacggactt gttgtcgtct tcgttgtcga caaatatacg cccaatggct
      361 tgacgagcct tgatattatc gcctaccttt acgtatatat cagttaagtt agaatataca
      421 gtacggtagt taccgtggcg tacaatcacc gtataattgc ttcctggaat ggagaaacgt
      481 tgggttactt ccccttcata tacagcacgt gcatccgaat ttggagcagt ttgaatataa
      541 atacctttat tatcagttgt tacatacttt agcacagcat gagcttgcac gccaaaacgc
      601 cctacaatta cgcctcgttc aacgggccat ggtaggcgac cttggttctt ttcaaagcct
      661 ccagaaagca gtttctgttc tttcgttaaa gcaaattctt gtgtgcctgc ggcttgtgct
      721 ttttgtgatt tgcgggcttc ttctgcaata atctgatcta tacgacgatt aatggcattg
      781 gcttgatctt gttgcttttt aagttctcta cgcaaatctt tttcttttct acttaattgc
      841 gaaacaaaat tgctttgctt acgttgttcg gtttgcaatt tagcattctc ctttcgtcta
      901 tcatttaata cagccacacg ctctaaacgg gctttttcta tttctgctaa tttgttttct
      961 aaatctgctt ttactttctc tatttcttgg gctttatttt gagcaaattc agcatactgt
     1021 tgtacatagc ggtatctgcg atacgattcg gcaaaacttt tagctgacaa aataaacatc
     1081 agggattcat atttggtttt tttgtaatac gaatggtaca ttaatttagc atactcgcct
     1141 tttaacttgt ccatttctgc ttgtttttct tgtgcctctt tgcgtaactg accaatattt
     1201 ctatctatat tacgaagttc ttggttcatc acattaatta aactctgacg agcattaatt
     1261 tctacacgca aaatattaat ttgagacaaa gaggtctttt tttgttcagc agttttacta
     1321 atcaacgaac tagtaatttc caattcttta agggcttttt tacgacgctc ttcaagctct
     1381 gctacagttt gagaaagagc aggtaatacc acactggcaa aaataaatag aatagaaagt
     1441 atttttttca taattattag ttaaaatatt ctttggcaat atcagccagc aaaatttgtt
     1501 tgtatctagg aggaataaca attgataaat ctatttgttg attaaactca actttttcga
     1561 tactataatc tatagcatat tgttgctgag aaaagagcat cacaatagcg tttttctttg
     1621 gatatactat cgtattgtat gtgtcaaact gctggtattc tgccatcata tagtgctttt
     1681 tttccgcatc ccttactgtt gttcgtagca aatgaagagc atcatttaca aggtattgaa
     1741 tcgcaaaatt actattttcg tacgatacta tgtgtagatt ttcgtctttt tggtataaaa
     1801 aatctgaaaa atcatctttt gtcgttacat ctactaatct attaaggata attgcttgca
     1861 acatacgaaa agaaactgga aacgttgacc caaacgacga aaaagattca gcaacatatt
     1921 gctgatttat tctgtcaagt aaaataagac tatcttgcgt aaggtgtatg cgagcaactt
     1981 ctattccaaa caaagcttgt atggaaattt gcatacaact atcaatcctt gctttgatag
     2041 agcctcgtag tggggtttgc tggttttgca ttggaataga aaagcttact ttcgaagcat
     2101 ttatcgtctg tatttgctgt tggtttgcca caattttctt caacggtact tgtataggca
     2161 ccacagactg agcagtgtct atttttgttg ttttacacgc caccaaaatg agcaacatac
     2221 atgctaacaa caatctgatt cctgatttat tcgacatact tttgggtgat aactttttgt
     2281 ttta
//
LOCUS       DAOPUR010000252         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_50411_length_2275_cov_3.781532, whole genome shotgun sequence.
ACCESSION   DAOPUR010000252 DAOPUR010000000
VERSION     DAOPUR010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50411_length_2275_cov_3.781532"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PKN90_05040"
     CDS             <1..907
                     /locus_tag="PKN90_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ85678.1"
                     /translation="VTTLFILIYSTDKFGNVMSSLKKFVTSVEKGDMNYKSITFPDTD
                     SGEIGNKIIAIYQQLEESKRKIDREKERNRELKQEMTNNIAHELKTPVSSIRGYLEIL
                     LGDKPVSEDKRKYFLERSYYQTLRLSDLINDVALITKMEEAATVFEKEKVNVRELAQE
                     AIEELSFRINPEQVDIQNNLENNLDIVGNKSLIFAIFRNLLENAINYAGEEITIGIKC
                     YNEDADYYYFLFYDTGCGVKPEYLEKIFDRFVRIDQGRSRRNGGTGLGLSIVKHAVMF
                     HNGAIEARNRKTGGLEFYFTLKKRK"
     gene            1124..1486
                     /locus_tag="PKN90_05045"
     CDS             1124..1486
                     /locus_tag="PKN90_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNZ85679.1"
                     /translation="MKKYLITVNQKQYEVEVVEVKQQVQTQKTTSGIKPSVTVSKPKE
                     TVAGANGHKVAAPMPSNIIKLLVNEGDVVNEGDNLLVFEAMKMENNLHSPVSGKIASI
                     KVKVGNSVAAGDTLIIIE"
     gene            1499..>2275
                     /locus_tag="PKN90_05050"
     CDS             1499..>2275
                     /locus_tag="PKN90_05050"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HNZ85680.1"
                     /translation="MGKVKITETALRDAHQSLIATRMRTEDMLPILEKMDNIGYHSLE
                     AWGGATFDSCIRFLNEDPWDRLRKIKQVVKKTPLQMLLRGQNLLGYKHYADDVVEYFV
                     QKAIDNGMDIIRIFDALNDPRNIETAIKACIKEGGHAQACVCYTTSPVHNLELFAKDA
                     KTLENMGAHSICIKDMAGLLKPYDAYELVKAIKETVNIPVQLHTHYTSGVASMTLLKA
                     IEAGVDVVDTAISPMAMGTSQPPTEPLVASLQGHERDTELD"
BASE COUNT          837 a          413 c          418 g          607 t
ORIGIN      
        1 tgtaactaca ctctttattc ttatatattc tactgataag tttggtaacg taatgagctc
       61 tcttaagaaa ttcgttactt cagtagaaaa aggtgatatg aactacaaaa gtataacatt
      121 ccctgatacg gactcgggcg aaattggaaa taaaattatt gccatatacc aacaattaga
      181 agaaagcaaa cgaaaaattg accgagaaaa agaacgtaac cgagagttaa aacaagaaat
      241 gaccaacaac attgctcacg aactaaaaac acccgttagt agcatccgtg gatatcttga
      301 aattttgctt ggagacaaac ctgtatctga ggacaaacga aaatattttc ttgaacgttc
      361 gtattaccaa actcttcgcc tttctgattt aattaacgat gttgctttaa ttacaaaaat
      421 ggaagaagct gcaaccgtat ttgaaaaaga aaaagtaaac gtaagagaac tagcacaaga
      481 agctatcgaa gaattgagtt ttcgtattaa tccagaacaa gttgacattc aaaacaactt
      541 ggaaaataac cttgatatcg ttggaaacaa aagccttatt tttgccatat ttcgtaattt
      601 actcgaaaat gccatcaact atgctggcga agaaattacc atcggcatca agtgttacaa
      661 cgaagacgct gactactatt atttcttgtt ttatgatacc ggttgtggcg taaaacctga
      721 atatctagaa aaaatattcg atcgctttgt acgtatcgac caaggacgga gtagacgcaa
      781 tggtggtaca ggactaggac tatccattgt aaaacacgct gttatgtttc ataatggagc
      841 tatcgaagcc cgaaatagaa aaacaggagg actagaattt tattttactc ttaaaaaaag
      901 aaaataaata ttatatcatt cttataaaaa tagaaacgct cttttctact gaaaactatg
      961 taattcggta aaaaagagcg ttttggtcgt taatattttc taaaagtaaa acagattact
     1021 aaaaaaactg tacttttgca taataaatta tctcttcaca agataaccta ctgagaataa
     1081 aaacatacat accaattaaa acattattaa agacaattta ttcatgaaaa agtatctcat
     1141 taccgttaac caaaaacaat acgaagtaga agttgtggaa gtaaaacaac aagtacaaac
     1201 gcaaaaaaca acttctggca tcaaacctag tgtaactgta tcaaaaccta aagaaacggt
     1261 ggcgggagca aatggtcata aagttgcggc acccatgcct agtaatataa ttaaattgtt
     1321 ggtaaacgaa ggagacgtag taaacgaagg agataatcta ttggtatttg aagccatgaa
     1381 aatggaaaac aacctacact ctccagtaag cggaaaaatt gcctctatca aagtaaaagt
     1441 tggcaattct gtagctgcgg gagacacatt aatcatcata gaataagaca ctacaacaat
     1501 gggaaaagtt aaaattacag aaacagcatt acgtgatgca catcaatcgc tgatagctac
     1561 acgtatgcgt acagaagata tgctccccat tcttgaaaaa atggataata tcggctacca
     1621 ctcgctcgaa gcttggggtg gtgctacatt cgattcttgt atccgtttcc taaacgaaga
     1681 cccttgggat aggctacgga aaattaaaca agttgtaaaa aagactcctt tacaaatgtt
     1741 acttagaggt caaaacctcc taggttacaa acattatgct gatgatgtag tggaatattt
     1801 tgtgcaaaaa gcgattgata atggtatgga tatcatccgc atatttgatg cacttaacga
     1861 ccctcgcaat attgaaaccg ctattaaagc atgtatcaaa gaaggtggtc atgcacaagc
     1921 gtgcgtttgt tataccacta gccctgtgca caatttagaa ctatttgcta aagacgctaa
     1981 aacgctcgaa aacatgggag cccattccat ctgtatcaaa gatatggctg gcttgttgaa
     2041 accatacgat gcgtacgaac tcgtaaaagc aataaaagaa accgttaaca ttcctgttca
     2101 attacatacg cattacacca gtggtgtagc ctctatgacc ttgctaaaag cgatagaagc
     2161 aggcgttgat gtggtggaca cagccatttc gcctatggcg atgggtacgt ctcaacctcc
     2221 taccgaaccg cttgttgcct cattgcaagg acatgaaagg gatactgagt tagat
//
LOCUS       DAOPUR010000253         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_50780_length_2265_cov_3.695475, whole genome shotgun sequence.
ACCESSION   DAOPUR010000253 DAOPUR010000000
VERSION     DAOPUR010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50780_length_2265_cov_3.695475"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKN90_05055"
     CDS             <1..684
                     /locus_tag="PKN90_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85681.1"
                     /translation="DQVSTGYFVHFLDDTYQVSSEIYYKWMQNQLDLKNGANIQANEY
                     IENELLVGNGRAYGLEIMLKKQYGALSGWIGYTLSRTENQIEGINNGNWYPARHDATH
                     DVSVVAIYELSKKWTLSSTWVYNTGNAVTFPSGKYQVDGETILLYTNRNASRMPHYHR
                     MDIGATRYFKKSKRFESSLNFSVYNAYGRKNAFTIDFEQDKNDPDKINAVKTYLFTFM
                     PSITYNFKF"
     gene            684..1490
                     /locus_tag="PKN90_05060"
     CDS             684..1490
                     /locus_tag="PKN90_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNZ85682.1"
                     /translation="MRKIIWLLVISFSITSCTEIIQLDLRNNKSEVVVEASMAAANEI
                     RVLLSRSVNLDESNTFPLLTGAQVSISDENNNTEVLTEIEPGVYQSITTKGIEGKRYF
                     LDLIVNNQSISATSTMPSIVPFTDMVVEKGSVFGPIGGNTNTDNTEYYEIKVLFNDPV
                     EEVNYYRFVEFINNEKQQEYIYDDRLNNGKDVEIVLFRFDRLLKTEDVVRIEMQTITK
                     EFYVYLSSLNDLRNSARSGSTPANPVTNMQGTSLGYFNTYTSQSKEYIIE"
     gene            complement(1492..>2265)
                     /locus_tag="PKN90_05065"
     CDS             complement(1492..>2265)
                     /locus_tag="PKN90_05065"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ85683.1"
                     /translation="IQNSNSAFLKKMPKWVISLLKKIIHQDEMNRILNKYEDCYGVDF
                     LPKIIEELQLTVQIEGEENLPADGRCFFAANHPFGFVDGLILTNTVGKKYGKLKAIGN
                     DSFLLAPHLKPIIAAVNVFGSNPKAYLLELENLFESDSPITHFPAGVVSRIRNGKIED
                     KDWQKSFIKQSVKYNRPIVPFHFTGRNSYLFYIIYLIRSFLGIKLNIELMLLPYEIFN
                     KKGKTTHVKIGKPIMPETFDNSKTSAEWAQYVKQQVYSL"
BASE COUNT          803 a          400 c          407 g          655 t
ORIGIN      
        1 gaccaagtaa gcactggcta ttttgtgcac tttttagatg acacctatca ggtatcgtct
       61 gaaatatatt acaaatggat gcaaaaccaa cttgatttaa aaaacggagc aaacattcaa
      121 gcaaacgaat atatcgaaaa cgaattatta gtaggcaacg gaagagcgta tggattagaa
      181 ataatgttga aaaaacaata cggtgcactc tccggatgga ttggatacac cttatcaaga
      241 acagaaaatc aaattgaagg gataaacaac ggcaattggt accctgccag acacgatgct
      301 actcatgatg tttctgtggt agctatttac gaactaagta aaaaatggac actatcaagc
      361 acgtgggtgt ataatacggg caatgcggtt actttcccaa gtggcaagta ccaagtagac
      421 ggagaaacta ttttgctcta tacaaacaga aacgcatctc gcatgccaca ctaccatcga
      481 atggatattg gagctactcg ctattttaaa aagtcgaaac gatttgaaag tagcctcaat
      541 ttttctgttt acaatgctta tggaagaaaa aatgccttta ccattgactt tgaacaagat
      601 aaaaatgacc ctgacaaaat caacgcagta aaaacatacc tatttacatt tatgccatca
      661 ataacctata attttaaatt ctgatgagga aaataatatg gcttttggtt atttctttca
      721 gtatcacttc gtgtacagaa ataatacagc tcgacttgcg gaacaacaaa tcggaagtag
      781 tggtagaggc atctatggct gcagcaaatg aaatacgtgt tttacttagc cgttcggtaa
      841 atttagatga atcaaacaca tttccgttgc taacaggtgc tcaggtgagt atatcagatg
      901 aaaataacaa tacagaagtg ttgacagaaa ttgaacctgg cgtttaccaa tcaattacga
      961 caaaaggaat agagggaaaa aggtatttcc ttgacttgat agtcaacaac caatcaatat
     1021 cagctacttc tactatgcct tctattgttc cttttacaga catggtagtc gaaaaaggga
     1081 gtgtttttgg tcctattgga ggaaatacga atacagataa cacagaatac tacgaaataa
     1141 aagtgctatt caacgatcct gttgaagaag taaattatta tcgctttgtg gaatttatta
     1201 ataatgagaa acaacaagaa tacatttatg acgatagatt aaacaatgga aaagacgtag
     1261 aaatagtatt gttccgcttt gatcgattgc taaaaacaga ggatgtagta aggatagaaa
     1321 tgcaaactat taccaaagag ttttacgtat acttgagttc gcttaacgac ttacgtaata
     1381 gtgctcggag tggctcaaca ccagccaacc cagttacaaa catgcaaggc acatcattgg
     1441 gctatttcaa cacgtacact agccaaagca aagagtatat catagaataa gttacagcga
     1501 atacacttgc tgtttgacat actgtgccca ctcagcggat gttttagaat tgtcaaaggt
     1561 ttcaggcata atgggtttgc caattttcac atgagtagtt ttaccttttt tattgaaaat
     1621 ctcgtatggc aatagcatta attcaatatt tagtttaatt ccgagaaaac tacgaattaa
     1681 gtaaatgata taaaataaat aagaattgcg tcctgtaaaa tgaaaaggaa caatggggcg
     1741 attatactta acagattgtt tgataaagct tttttgccaa tctttatcct caatttttcc
     1801 gttacgaata cgcgacacca cacctgcagg aaaatgggta atgggtgagt ctgattcaaa
     1861 caaattctcc aactccaaca aatacgcttt tggattagac ccaaatacat ttacagcagc
     1921 aataatgggt ttaagatgcg gtgctagcaa aaaagaatcg ttacctatag cttttaactt
     1981 accatacttt ttaccaacag tattggtaag aattagccca tccacaaaac caaagggatg
     2041 atttgcagca aaaaaacatc gtccgtcagc tggtaaattt tcttcccctt ctatctgtac
     2101 agttaattgt aattcttcaa taatcttagg caaaaaatct acaccatagc aatcctcata
     2161 tttatttagg atccgattca tttcatcttg gtgtataatc tttttgagta aagaaattac
     2221 ccactttggc atttttttta aaaaagcaga attgctattc tgtat
//
LOCUS       DAOPUR010000254         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_51054_length_2257_cov_3.181199, whole genome shotgun sequence.
ACCESSION   DAOPUR010000254 DAOPUR010000000
VERSION     DAOPUR010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51054_length_2257_cov_3.181199"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PKN90_05070"
     CDS             <1..751
                     /locus_tag="PKN90_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85684.1"
                     /translation="LLFVNTLAPSKTDVFTDFDASFELTASPVIAFTDVDYISVGTDF
                     IHTYSDDVLMPFYISGAKRSFIAIDGEQILDYFRVDYTNSQISIVNDSTYKVSVQYQH
                     SLSISQNEPNLPASLQTKQLTQTIVYNFVLGKLTIEQHANITNKWGAYYDDFMDTHHP
                     ESLGSTRTEHTKISLIGVNTFGMVDSDRLFFGTENTADTRKAILSMSDTSNATYYYSN
                     GEVSGTSSRSYVSTDFSSPSVKIYLWVHYDQ"
     gene            936..>2257
                     /gene="thrS"
                     /locus_tag="PKN90_05075"
     CDS             936..>2257
                     /gene="thrS"
                     /locus_tag="PKN90_05075"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNZ85685.1"
                     /translation="MINITFPDGSVRQYEKGTTGLQIAESISSRLAQDVIAVGVNDQT
                     TEINRPITTDASIKLYKWDDAEGKHAFWHTSAHLMAEALQELYPHVKFGIGPAIENGF
                     YYDIDTADMVIRENDFPTIEAKITEIVARKENVVREEISKADAIKLFTEKDEPYKIEL
                     ISELEDGTISLYRQGNFVDLCKGPHLANTADIKAFKLLSVAGAYWRGDEKRKQLTRIY
                     AISFPKKKMLDEYLLLLEEAKKRDHRKIGKELELFTFSQSVGQGLPLWLPKGTMLRDQ
                     LEDFLKKVQKKYGYLPVRTPHIGQKELWITSGHYAKYGKDSFQPIHTPQEGEEYLLKP
                     MNCPHHCEIYKVTPRSYKDLPIRYAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCR
                     PDQLKDEFMKVMDIIFIIFKALNFENFEAQISLRDPNNKEKYIGSDEN"
BASE COUNT          783 a          449 c          381 g          644 t
ORIGIN      
        1 tttactgttt gtcaacacat tagctccatc taaaacagat gttttcactg attttgatgc
       61 ttcgtttgaa ttaaccgcat ctcccgttat tgcctttaca gatgttgact atatttctgt
      121 aggtactgat tttattcata cgtatagcga cgatgtgctc atgccatttt acatctctgg
      181 agccaaacgc agtttcattg caatagatgg agaacaaatc ttggactatt ttagggtgga
      241 ctatacgaat tctcaaataa gcatcgtaaa cgactccaca tacaaggttt cagtacaata
      301 ccaacactca ttaagcatta gtcaaaatga accaaatcta ccagcctcac tccaaactaa
      361 gcagctaaca caaacaatag tatataattt tgtactagga aaattaacca ttgaacagca
      421 tgccaacatc accaataaat ggggagcata ttacgacgat tttatggata cacaccaccc
      481 cgaatcgctt ggttcaacac gtaccgaaca cactaaaata tccctaattg gagtcaatac
      541 atttggaatg gtagattctg accgcttatt ctttggtaca gaaaatacag ctgacacccg
      601 taaagctatc ctctcaatgt ccgatacatc aaacgctacc tactattaca gtaatggcga
      661 agtaagtgga acatcatcaa gaagctatgt aagcacagat ttcagttctc cttctgtaaa
      721 aatttacctg tgggtgcatt atgatcaata aaagtgctta ttcattattt cccgtttcaa
      781 ctagtactgc ttgaaacggg ttttttatac ataataatca gattttaaac gttacaaaac
      841 cataaaatag caaacgataa atagttttca aaaaatatta tttacctttg cagtcattac
      901 aaaattgaat caatcacatt cgataaacta tctatatgat taacattaca tttcccgacg
      961 gttctgttcg tcaatacgaa aaaggaacta ctggcttaca aatagccgag agcatcagct
     1021 cacgcctagc acaagacgtg attgctgttg gagtaaacga ccaaactact gaaattaatc
     1081 gcccaataac gacagacgct tctattaaat tatacaaatg ggatgatgct gaaggcaaac
     1141 atgctttttg gcacacctct gctcacttaa tggctgaagc cctacaagaa ttgtatcctc
     1201 acgtaaaatt tggcatcgga ccagctatcg aaaatggttt ttattacgat atagatacag
     1261 ctgatatggt aattcgtgaa aatgattttc caactattga agcaaaaata actgagattg
     1321 ttgcacgcaa agaaaatgta gtgcgtgaag aaatatctaa agcagatgct attaaattat
     1381 ttactgaaaa agatgaaccc tacaagattg aattaatcag cgagcttgaa gatggcacca
     1441 ttagcctgta ccgtcaaggg aattttgttg atttatgcaa aggacctcac ttggctaata
     1501 ctgctgatat caaagcattt aaattgcttt ccgtagctgg agcatactgg cgtggtgacg
     1561 aaaaacgcaa acaattaact cgtatttatg ccatttcgtt tccaaagaaa aaaatgcttg
     1621 acgaatattt gttgttatta gaggaagcaa aaaaacgcga ccacagaaaa attggtaaag
     1681 aattagaact attcaccttc tctcaatctg ttggtcaagg cttacctctt tggttaccca
     1741 aaggtacgat gctacgtgac caattagaag acttcttgaa aaaagtacaa aaaaaatatg
     1801 gttatttgcc tgtacgtaca cctcatatcg gacaaaagga attgtggatt acatctggac
     1861 attatgcaaa atatggtaaa gattcatttc aacctatcca cacacctcaa gaaggcgaag
     1921 aatatttact taaaccaatg aactgtcctc accattgcga aatatataag gtaactccac
     1981 gttcgtacaa agatttaccc atacgatatg ctgaatttgg tactgtttat cgatacgaac
     2041 agagtggtga gttacatgga ttaacacgcg ttcgcagctt tacacaagac gatgcacata
     2101 ttttctgtcg ccctgaccaa ctcaaagacg aatttatgaa agtaatggat attatcttca
     2161 tcatcttcaa agcgttaaat ttcgaaaact ttgaagcaca aatttcattg cgcgacccaa
     2221 acaacaaaga aaaatacata ggtagcgacg aaaactg
//
LOCUS       DAOPUR010000255         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_51128_length_2255_cov_3.013182, whole genome shotgun sequence.
ACCESSION   DAOPUR010000255 DAOPUR010000000
VERSION     DAOPUR010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51128_length_2255_cov_3.013182"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1789
                     /gene="rny"
                     /locus_tag="PKN90_05080"
                     /pseudo
     CDS             262..1789
                     /gene="rny"
                     /locus_tag="PKN90_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
     gene            1801..2199
                     /gene="ssrA"
                     /locus_tag="PKN90_05085"
     tmRNA           1801..2199
                     /gene="ssrA"
                     /locus_tag="PKN90_05085"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          810 a          386 c          476 g          583 t
ORIGIN      
        1 tgataatggt agcttttgaa ttatcgttac ttagtttgag attggaagaa gaaagagata
       61 attctccatt tatatctacc attcaagcat tggataatcg attggctgaa tacctgaaaa
      121 acaatcaact agacactaaa aacgaggaat aatatactat tcagtttttt acgcagattt
      181 tataacattg caattctcaa aatcaactct gttgtttttg tttttgcaat gtttttattt
      241 atttactaat acctattata tatgacaatt atttatatca ttctggcatt tgttggcggt
      301 gctacggtat cggcaatagt ggtgcaaata gtgcaaaaag cacgagccaa aagcattatt
      361 gatgcggctc aaattgaggc tgaaactctt aaaaagagta agctattgga aaaaagaaaa
      421 atacatccaa ttaaaagcag agtatgaaaa acaaatgacc gctcgtaatg ctaaaattca
      481 atcgatagag gctaaaatta agcaaaaaga gctgtttata gagaatgagg aaaaacaact
      541 ggagaaaaaa gcagcggaac tagaaagtat aaagctaaac tatgaaaatc agttgagttt
      601 gaatgaaaag aaagaagaag aattagatag actgcatcac caagcagtac aaaaattaga
      661 agctgttgcg ggtttatctg ccgaagaagc aagaaacaac ctaatagaat cgttaaagaa
      721 tgaagccgaa acaacagcag cctcgtacat taacgaaata atggacgaag ctaaaatgac
      781 agccaacaaa gaagctaaaa aaattgtggt aaataccata caacgtgtgg caacagaaac
      841 ggcaatcgaa aattcagtta cggtatttca tatcgaatct gacgaattaa aaggtcgtat
      901 cattggtcgc gaaggtagaa acatacgcac attagaggca gctacaggag tagaaattat
      961 tgtagatgat accccagagg cgattgtact atcagcattt gacccagtaa gacgcgaaat
     1021 tgcacgttta tcgttacacc aattggtaac agatggacgc attcaccctg ctcgtatcga
     1081 agaggtagta gctaaggtaa ccaaacaaat tgaagaagaa atagtagaaa ttggtaaacg
     1141 taccaccatt gatatgggtg tgcatggatt gcatcctgaa ttggttcgct tgattggtaa
     1201 aatgaaatac cgttcgtctt acggacaaaa cttattgcaa cactcgcgcg aaacggctaa
     1261 tttatgtgcc gttatggcag ctgaattggg cttaaatccg aaaaaagcac gtagagcagg
     1321 acttttgcac gatataggca aagtggcaga cgaggaacca gaattaccac atgcactttt
     1381 aggcatgaaa cttgcagaga aatacaaaga aaaacccgat atttgcaatg ccattggagc
     1441 tcaccacgac gaggtagaaa tgcaaaccct actcgctcct attgtgcaag cgtgtgatgc
     1501 tatttcgggg gctcgtccag gtgctagacg cgaaattgta gaagcgtata tcaaacgctt
     1561 aaacgactta gaaaatatcg ccctatcgta tccaggtgta atgaaaacct acgccataca
     1621 agcaggtaga gagctacgtg taattgtagg tgcagataaa attgatgacc aagcaacaga
     1681 aagactttcg caagaaattg cgaaacgcat tcaagacgaa atgacctacc ctggtcaagt
     1741 gaaaattacc gttatcaggg aaatcagatc ggtaagtgtt gctaagtagt acataacatt
     1801 gggcttgact ggaattgaca gcgagtaaaa ttggtatgta agcatgcaga gctttgtatg
     1861 agtagctctt taatctgaac atacaacaat ctaattggcg aaaataacta cgctcttgct
     1921 gcttaatcga ataacagtag attagcttaa tttcggtaca aggtgctgaa acaagacatc
     1981 tctcaaatgc ccactccttg aggcggtttg atatagaggt gctaactaaa tcagggatag
     2041 cttatcatcg cctcgtatgg taagtgaaaa taaagaggat aaggttgtag ttggtggttt
     2101 cggtcttgct acaacacgaa aataaaagcg aaaataagca tgtagaaagc gtattaattt
     2161 cttgtttgga cgagggttcg actccctcca ggtccaccaa ctaaacaaaa ccactgataa
     2221 tctatttatt atcagtggtt ttgttatttt ttatg
//
LOCUS       DAOPUR010000256         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_51316_length_2250_cov_2.786788, whole genome shotgun sequence.
ACCESSION   DAOPUR010000256 DAOPUR010000000
VERSION     DAOPUR010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51316_length_2250_cov_2.786788"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /gene="gmk"
                     /locus_tag="PKN90_05090"
     CDS             complement(<1..456)
                     /gene="gmk"
                     /locus_tag="PKN90_05090"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNZ85686.1"
                     /translation="MQGKLIVLSAPSGAGKTTIMRYLLQQDLSLSFSISATTRQPRGT
                     EEHGKDYYFFSVEDFRQKMADGAFVEYEEVYPGCFYGTLKSEVERLTSQGKHVVFDVD
                     VVGGVNIKKMYGKRALTIFVQPPSIEALQQRLQTRAIDTPEMIATRIAKA"
     gene            complement(478..1353)
                     /locus_tag="PKN90_05095"
     CDS             complement(478..1353)
                     /locus_tag="PKN90_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNZ85687.1"
                     /translation="MIQSMTGYGKAVSEYENKKIVAEIKSLNSKQLDLSTRVSSFYRE
                     KDIEIRNEISQRVERGKVEFSVYVENATSDVTSKINTTIFQQYVEQLSQLAEKSGIEK
                     PTNWFEIILRFPEVMKNETAELTDEEWAVVKTTLLASIEQLQLYRKEEGKALHAVLLQ
                     KIDNIQQYLEQTVQYESERIDKIKQRLHENLKAIDDKIIVDENRLEQELIYYIEKLDV
                     NEEKERLKHHLAYFKSTMQEPTSSGKKLGFIAQEIGREINTLGSKSNQSDMQRLVVLM
                     KDELEQIKEQVLNVL"
     gene            1502..2191
                     /gene="tsaB"
                     /locus_tag="PKN90_05100"
     CDS             1502..2191
                     /gene="tsaB"
                     /locus_tag="PKN90_05100"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNZ85688.1"
                     /translation="MPILLHLETSTTVCSVCLSENGKIVYEVVDDKGQNHSELLGVFV
                     QKALQEAKKQHLQLDAVAVSSGPGSYTGLRIGVSMAKGLCYGFSIPLIAINSLKILAQ
                     GVVANHSIEHDAILCPMIDARRMEVYTAFFDARLNILKPTWAEVLHADTFETLHDKKV
                     YIFGNGAEKSIGIVQHPNIQFLPHQTPLASSMIVLAEEAFQQQQFEDMAYFEPFYLKE
                     FMTSIPKNKLA"
BASE COUNT          623 a          442 c          414 g          771 t
ORIGIN      
        1 agctttggct atgcgtgttg ctatcatttc gggggtatcg attgccctag tttgcaagcg
       61 ttgttgcaag gcttcgatag atggcggttg cacaaaaata gtgagtgctc gcttgccata
      121 catttttttt atattcaccc ctcccactac atctacatca aatactacgt gtttgccttg
      181 cgaggttagg cgttccactt ccgacttgag tgtgccataa aaacagccgg gatatacttc
      241 ttcgtactct acaaatgctc catctgccat tttttgtcga aaatcttcta ctgaaaaaaa
      301 ataatagtcc ttgccatgtt cttctgtgcc tcgtggctgg cgtgtagtgg ctgagataga
      361 aaacgataag gataagtctt gttgcaataa ataccgcata atggtagttt tgcctgctcc
      421 cgatggtgcc gaaagtacga ttaatttgcc ttgcacagtt gtttccataa gctgttgtta
      481 caatacattc aatacttgtt ctttgatttg ctcaagctca tctttcatca gcactaccaa
      541 gcgctgcata tcgctttgat tggattttga gcctaaggta ttaatttctc tgccaatttc
      601 ttgtgctata aagcctagtt ttttacctga cgatgttggc tcttgcatgg tagatttaaa
      661 atatgccaaa tggtgtttta agcgttcttt ttcttcgttt acatctaatt tctcgatgta
      721 gtaaatcaat tcttgctcta gcctattttc atctacaatt attttgtcgt caatggcttt
      781 taggttttcg tgcaagcgtt gtttgatttt atctatacgt tctgattcgt attgcacggt
      841 ttgttccaaa tactgttgta tgttgtcaat tttttgtagc aacactgcgt gcaatgcctt
      901 cccttcttct tttctataca gttgcaattg ttcgatactg gcaagcaggg tagtttttac
      961 tacagcccat tcctcatcgg tcagttcggc tgtttcattt ttcatcacct ctggaaaacg
     1021 gagtattatc tcaaaccaat tggtcggttt ttctatgccc gatttttcgg ctaattgaga
     1081 taactgctct acatactgtt gaaaaatagt ggtattaatt tttgatgtaa catcgctggt
     1141 ggcattttct acatacaccg aaaactctac tttacctctt tctacgcgtt ggcttatttc
     1201 atttcttatc tcaatatctt tttcgcgata aaaagaagac acgcgtgtag aaaggtctaa
     1261 ttgtttacta ttgagcgatt taatttcggc tactattttt ttgttttcgt actcacttac
     1321 agctttacca tagccggtca tcgattgaat catacctatc tgtttagttt attgttgcac
     1381 acaaaaatac tgcttttatc tacaaaaaca gccatatcct acgaataaat caaaaatttt
     1441 agttgcaatc atcataattc agctaaaaaa ttgtaccttt gtacgttaca aaagacatga
     1501 tatgccgata ctattgcatc ttgaaacctc tactactgtt tgttctgtct gcttatccga
     1561 aaacggaaaa attgtatatg aagtagttga tgataaaggg caaaatcaca gtgagttact
     1621 gggtgttttt gtacaaaaag ctctgcaaga agcaaaaaaa caacatctgc aactcgacgc
     1681 ggttgctgta agtagcggac caggttccta cacggggtta cgcattgggg tgtcaatggc
     1741 taaaggattg tgttatggct tttctattcc tcttattgct atcaatagct taaaaatact
     1801 ggcacaaggg gtagtagcca atcattcgat tgaacacgat gcaattttat gcccaatgat
     1861 tgatgccaga cgaatggagg tatatacggc tttttttgat gcacgattaa atatactgaa
     1921 accaacatgg gctgaagtgc tacatgccga cacattcgaa accttgcacg ataaaaaagt
     1981 atatattttt ggcaacggag ccgaaaaatc aataggcatt gtgcaacatc caaacataca
     2041 gtttttgcca catcaaacgc ctttggcttc gagtatgatt gtgcttgctg aggaggcttt
     2101 tcaacaacaa caatttgaag atatggctta ctttgaacca ttttacctaa aagaatttat
     2161 gacttctatt cctaaaaaca aattagctta gtaaaattat atgacaacaa cttacgatac
     2221 tgcaatggaa tattacacag agaaagagaa
//
LOCUS       DAOPUR010000257         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_51418_length_2247_cov_3.478558, whole genome shotgun sequence.
ACCESSION   DAOPUR010000257 DAOPUR010000000
VERSION     DAOPUR010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51418_length_2247_cov_3.478558"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKN90_05105"
     CDS             complement(<1..242)
                     /locus_tag="PKN90_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNZ85689.1"
                     /translation="MNTPLEKPYTFDRVIRIVITLVILLALFFLIKKLSAVLLPFLIA
                     LLIAYLLQPVVHFFQYTLKFKFRILAVISTLTLFFGA"
     gene            complement(345..1628)
                     /gene="hisD"
                     /locus_tag="PKN90_05110"
     CDS             complement(345..1628)
                     /gene="hisD"
                     /locus_tag="PKN90_05110"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNZ85690.1"
                     /translation="MYYIQYPEKKERASLLARPTFDTTNLFDTVNHILVDVQKRGDKA
                     VIEYEEKFDKIHLDVLQVSDNELASAETKISKELKDAILIAKKNIANFHAAQVYSDVT
                     IETMPGIHCWQKNLPIEKVGLYIPGGTAPLFSTVLMLAIPAQIAGCKEIILCSPPNSN
                     GDIHPAILFAAHISGVSKVYKIGGVQAIAAMAYGTETVPKVYKIFGPGNQYVTAAKQL
                     VSLKDVAIDMPAGPSEVEVIADETANPAFVAADLLSQAEHGADSSAILLTTSNELATA
                     VQQEIQQQLPLLSRAEIAVKALNNSKIILLHTMEDIIEWTNEYAPEHLIISTKNYTDI
                     ANRITNAGSIFLGYLTPESAGDYASGTNHTLPTNGYAKAYSGVNLDSFKKKITYQELT
                     KEGIQAIGGTIEVMAENELLTAHKNAVTLRLQSLV"
     gene            complement(1650..>2247)
                     /gene="hisG"
                     /locus_tag="PKN90_05115"
     CDS             complement(1650..>2247)
                     /gene="hisG"
                     /locus_tag="PKN90_05115"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNZ85691.1"
                     /translation="DFSKCRLSLAIPKSIDYQGVHWFEGKKIATSYPVILENFLKKHN
                     IQAEIHVITGSVEIAPGIGLADGIFDIVSSGSTLVSNNLKEVEVVMKSEAILIANKQL
                     NSEKRAILEELLFRFNAYKNAEGKKYIILNISNDKIDAVCKLLPGMKSPTVTPLAEEG
                     WSSLHSVIEEKTFWDIINQLKALGAEGILVVPIEKMIL"
BASE COUNT          685 a          415 c          373 g          774 t
ORIGIN      
        1 gctccaaaga agagtgtcaa tgtactgata acggctaata ttctgaactt aaatttcaaa
       61 gtatattgaa aaaaatgaac gactggctgc agtaaatagg caattagcaa tgctattaaa
      121 aagggaagaa gtactgcact caatttcttg attagaaaga ataatgcaag caaaataacc
      181 aatgttatta caattcgaat aacacgatcg aatgtatagg gcttttctaa aggggtattc
      241 attgggttat agtattgcta tccattacaa gttagtatta ttctgaccac aaacatacaa
      301 ataataaaac atatattcat acaaaacgcc caaaaatatc aatactatac aagtgattgt
      361 aagcgtagcg taacagcatt tttatgtgct gttaacaatt cattttcagc cattacttca
      421 atagtgcctc ctatggcttg tatgccttct tttgttaatt cttgatacgt aatttttttc
      481 ttaaaactat ctagatttac accactatat gcctttgcgt atccattagt aggtaaggtg
      541 tggtttgtgc ctgaagcata atcgccagca ctttcaggtg ttaagtaacc caaaaatatt
      601 gaacctgcat tggtgattct attggctata tctgtgtaat tttttgtaga gataattaaa
      661 tgttcaggag cgtactcgtt tgtccattca atgatatcct ccattgtatg tagcaaaatt
      721 attttgctat tattcagtgc tttaacggca atttcagcac gcgacaaaag aggaagctgt
      781 tgctgtattt cttgttgcac agcagttgct aattcattgc tagttgttag caaaatagcg
      841 gaactgtctg ccccatgctc agcttgcgaa agcaaatctg cagcaacaaa cgcagggtta
      901 gctgtttcgt cagcaattac ttctacttcc gatggaccag ccggcatatc aatagcaaca
      961 tcttttaaac ttacaagttg cttagcggct gttacatatt gatttcctgg accaaaaatt
     1021 ttgtatactt ttggtacggt ttcagtgcca tatgccattg ctgcaatagc ttgcactcca
     1081 ccaattttat ataccttact tactcccgaa atatgtgctg caaataaaat ggctgggtgt
     1141 atatctccat tgctatttgg tggtgaacaa agaataattt ctttgcaacc agcaatttga
     1201 gctggaattg ctaacatcag taccgtagaa aataggggag cagtacctcc aggaatatat
     1261 aatccaactt tttcgattgg tagatttttt tgccaacaat gtataccagg catagtttca
     1321 attgtaacat cgctatacac ttgggctgca tgaaagtttg caatattttt ttttgctatt
     1381 aaaatagcat cttttaattc tttgctgatt tttgtttctg cacttgctag ctcgttatcg
     1441 cttacttgca atacatccaa atgaattttg tcaaattttt cttcgtactc gataacagcc
     1501 ttatctccac gtttttgtac gtcgactaaa atatggttaa cggtatcaaa taaatttgtt
     1561 gtgtcaaagg ttggacgtgc aagtaaactt gccctctcct ttttttctgg atattgaata
     1621 tagtacataa acaaaaattt tgtaaattat tacagaatca ttttttcaat tggaacaacc
     1681 aatattcctt ctgctcctaa tgctttcagt tggttgatta tgtcccaaaa tgttttttcc
     1741 tcaattaccg aatgtagtga tgaccatcct tcttctgcca atggcgtaac agtaggtgat
     1801 ttcatgccag gcaaaagctt acatacggca tctattttgt cattagaaat atttagaata
     1861 atgtattttt tcccttctgc atttttatac gcattaaaac ggaataataa ttcttctaaa
     1921 atggctcttt tttcactatt aagttgtttg ttggcaataa gaatagcttc agatttcata
     1981 accacttcca cttcttttaa gttattgctc accaatgtgc taccagaact tacaatgtca
     2041 aaaatgccat cagccaagcc aatgccagga gcaatttcta ctgaaccagt aattacatgt
     2101 atttcggctt gtatgttgtg ttttttaaga aaattctcta aaatgacagg atacgaagtg
     2161 gcaattttct tgccttcaaa ccaatgaacc ccttgataat ctattgactt aggtatagct
     2221 aatgataaac gacatttgct aaaatcc
//
LOCUS       DAOPUR010000258         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_51428_length_2247_cov_2.609033, whole genome shotgun sequence.
ACCESSION   DAOPUR010000258 DAOPUR010000000
VERSION     DAOPUR010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51428_length_2247_cov_2.609033"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PKN90_05120"
     CDS             <1..267
                     /locus_tag="PKN90_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ85692.1"
                     /translation="HQYTLKLKNVDRAALQKALEAKGIPAMVYYPVPLHMQKAYQDAR
                     YKEGDFPVAEALSACVISLPMHTELDEEQLNYITSEFLTAINNQ"
     gene            269..841
                     /locus_tag="PKN90_05125"
     CDS             269..841
                     /locus_tag="PKN90_05125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNZ85693.1"
                     /translation="MSYFKHESAYVDEGCQIGNGTKIWHFSHIMPNCTIGESCNIGQN
                     VVISPDVVLGRNVKIQNNVSVYTGVICEDDVFLGPSMVFTNVINPRSHVNRKAEYAKT
                     LIKKGASIGANSTIVCGNEIGEFALIGAGAVITKNILPYALVVGNPARQIGWVSEYGH
                     RLNFDDKGIAICPETQQEYQLSNNTVKRIK"
     gene            838..1860
                     /locus_tag="PKN90_05130"
     CDS             838..1860
                     /locus_tag="PKN90_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ85694.1"
                     /translation="MSNKIKFAVVGLGHIGKRHAEMIRRNASSELVAVCDILPKDKID
                     PEITETYYSDFDEMLAKQPDIDVVNVCNLNGHHAEYAIKALQAKKHVVLEKPIALSKS
                     DAEKVVFKSLEMSRSVFCVMQNRYSPPMAWLKELVENDTLGKIYFVKVDAFWNRDEKY
                     YKRGSWHGTNKLDGGTLFTQFSHYVDMLYWLFGDITTIRGKFANCSHGDLIEFEDTGC
                     VDFEFINGGMGSLNYSTSVWDKNLESTVTIIGEKGTVKIAGQYMDQVVHCHIKDYEMP
                     ELKQPELNNDFGGYKGAAQNHGFVIQNVIDTLKAKGAIGTNLLEGMKVVDIIERIYQE
                     RDRSFK"
BASE COUNT          809 a          350 c          428 g          660 t
ORIGIN      
        1 caccaatata cattaaaatt aaaaaatgta gatagagctg ctttacaaaa agcattagaa
       61 gcaaaaggaa ttccagctat ggtatattat ccagtaccat tgcatatgca aaaagcatat
      121 caagatgcac gatacaaaga aggcgatttt ccggtagcag aagcattaag tgcctgcgtt
      181 attagtttgc caatgcatac cgaacttgac gaagaacaat tgaattacat tacatctgaa
      241 tttttaaccg ctataaacaa tcaataatat gtcatatttt aaacacgaat cagcttatgt
      301 agatgaaggt tgccagatag gaaatggcac taaaatttgg catttttctc acatcatgcc
      361 taactgtaca ataggcgaaa gttgcaatat tggacaaaat gtagtgatat ctccagatgt
      421 agttttgggt agaaatgtaa aaatacagaa caatgtatca gtttatactg gtgtaatatg
      481 cgaagatgat gtatttcttg gtccatcaat ggtattcaca aacgtaatta acccccgtag
      541 tcacgttaat cgcaaagctg aatatgctaa aacacttata aaaaaaggag ctagtattgg
      601 agctaattct actattgtat gtggcaacga aataggcgaa tttgccctaa tcggagctgg
      661 tgcggttatt acaaaaaaca ttcttcctta cgcattggta gtaggcaatc cggcacgtca
      721 aatagggtgg gtaagtgaat atggtcatcg ccttaatttt gacgacaaag gaatagctat
      781 ttgtccagaa acgcaacaag aataccagtt aagtaataac actgtaaaac gaataaaatg
      841 agtaataaaa taaaatttgc agtcgtagga ttaggtcaca taggcaaacg tcatgccgaa
      901 atgataagac gcaatgctag ttcagaacta gtagcagtat gcgatatttt accaaaagat
      961 aaaattgatc cagaaattac tgaaacgtat tattcagatt tcgatgaaat gcttgctaaa
     1021 caaccagata ttgatgttgt aaatgtatgt aacctcaatg gtcaccatgc tgaatatgcc
     1081 atcaaagctt tacaagcaaa aaaacacgta gtgctcgaaa aaccaatagc attaagcaaa
     1141 tcagatgctg aaaaagtagt attcaaatcc ttggaaatgt ctcgttcggt attttgtgtt
     1201 atgcaaaatc gctattcacc gccaatggct tggttgaaag aattagttga aaatgataca
     1261 cttggcaaaa tttattttgt aaaagtagat gcattttgga accgcgacga aaaatactac
     1321 aaacgtggta gctggcatgg aactaacaaa ttagatggag gaaccttgtt tacgcaattt
     1381 tcgcattatg tagatatgtt gtactggtta tttggcgata tcaccactat tcgtggtaaa
     1441 tttgctaatt gctcgcacgg tgatttaatc gaatttgaag atactggttg cgtcgatttc
     1501 gaatttatta acggaggtat gggttctctg aactattcga cttctgtttg ggacaaaaac
     1561 cttgaaagta ctgttaccat cattggagaa aaaggaactg ttaaaattgc aggtcaatac
     1621 atggatcaag tagttcattg ccatattaaa gactacgaaa tgccagaatt gaaacaacct
     1681 gaattaaaca acgattttgg tggatacaaa ggtgctgccc aaaaccatgg ttttgttatt
     1741 caaaatgtaa tagataccct gaaagccaaa ggagcgattg gtaccaactt gctcgaaggt
     1801 atgaaagtag tagatattat tgaacgcatt tatcaagaaa gagaccgttc attcaagtaa
     1861 acgaaacgtg tttcattact cttatcttcc cctcgctttc agcgagggga tttttgtttt
     1921 agcgttttta acgcattcta ataataataa taatacattg ttgaaaacga gggtgaggag
     1981 tataagcgtt taaaactcgt tattatattc tacatttttg aaaaggaaaa agagtatgca
     2041 gtagctaaaa aaatacgtaa atagttgtat atttcgatac aatatagtat ctttgataat
     2101 ggtattatta taccaataat aacttattaa cgaattaagt agcattatga tactattaaa
     2161 ccaaaggtca tttagtgaaa tatgtgttga tatggctcaa aaagttaaac aacggagatt
     2221 agaactaaac cttactcaaa aaggttt
//
LOCUS       DAOPUR010000259         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_51919_length_2232_cov_2.950850, whole genome shotgun sequence.
ACCESSION   DAOPUR010000259 DAOPUR010000000
VERSION     DAOPUR010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51919_length_2232_cov_2.950850"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..917
                     /locus_tag="PKN90_05135"
     CDS             306..917
                     /locus_tag="PKN90_05135"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009237091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNZ85695.1"
                     /translation="MISVYKIKPKFQNALSPILKLLHKWNISANQITISSILLSLCIG
                     IFFWFADFNTYFFLALPIGLFIRMALNALDGMMARTYNQQSKIGEVLNEIGDVISDIL
                     IFFPLLKFESEHIYLIVIFLCLGIINEFSGFMGKVISNIRRYDGPMGKSDRALIIGIY
                     GIISFIGINFSNYTFYIFMLINLLLVISTITRIKRALDYGKDI"
     gene            901..1854
                     /locus_tag="PKN90_05140"
     CDS             901..1854
                     /locus_tag="PKN90_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNZ85696.1"
                     /translation="MEKIFRIDYFQKNELLIVVAIIIGILVFASLLFYVIGLLKPDAK
                     LQELKARTKSWWIMASIFIFATIVNAKISYVAIAFLSFVAFRELYSLMGFRESDRRAI
                     FWAFLSIPIQYYLAFIGWYGAFIIFIPVVMFLLLPFRFVLKGETDGIIKSMASLQWLL
                     MLTVFGISHLAYLLSLPELPNFNSGGRGLLLFVVFMTEINDVMQFVWGKLFGKKKIIP
                     KISPNKTWEGFIGGVISTTIIGYCLGFLTPLNGYELVFVSFMIAFTGFIGDVVMSSIK
                     RDIGVKDSGNLIPGHGGVLDRIDSLMYTAPVFFHLVYYIAY"
     gene            1854..>2232
                     /locus_tag="PKN90_05145"
     CDS             1854..>2232
                     /locus_tag="PKN90_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ85697.1"
                     /translation="MKKLILQIVYNVIIRTFLRVIIGVKFENPNFLLTEKQFVIIANH
                     NSHLDTMAVMASLPRKIIHKVKPVAAMDYFGKTKLQEKLSNYFINTLLVKRITATEKG
                     GENPIRQMIKELDKGHSLIIFPEG"
BASE COUNT          752 a          276 c          387 g          817 t
ORIGIN      
        1 ataacagcag atacttttta taatttgagt ttagtgtata agaataaagg agatgttgaa
       61 aatgagctta attatttgac taaatcaaat aatttagtag agtctgattt taaaactaaa
      121 cggattaaat atctaaaatt gaaataaaaa caccagctaa cacgcggtat aaaaaattgt
      181 cgagaaaata ggttattcaa aggttgtagc tcgctttaac ttttgtgtaa attgatagga
      241 aatcgctcgc tattggcaac ttttcatact gctaacgtta gcaaaaaaaa attaaaaata
      301 gacatatgat ttcagtttat aaaataaaac caaaatttca gaatgcactt agccctattc
      361 taaagcttct acataaatgg aatataagtg caaatcagat aacaatttct tccattttat
      421 tatcgctatg tattggaata tttttctggt ttgctgattt taatacatat ttctttcttg
      481 cattacctat tggtttgttt atacgaatgg ctcttaatgc tttagacgga atgatggcaa
      541 gaacctataa tcaacaatct aaaataggtg aagtgctaaa tgaaattggg gatgtaatat
      601 ctgatatttt aatatttttc cctcttctaa agtttgaatc tgagcacata tatcttatcg
      661 ttattttttt atgccttgga attattaatg agttttctgg ttttatgggg aaagttatat
      721 ctaatatacg cagatacgat ggacctatgg gcaaaagtga cagagctctt attataggta
      781 tttatggtat tattagcttt attggaatta acttctcgaa ttatacattt tatattttta
      841 tgttgattaa tttacttttg gttataagta caataacacg aattaaaaga gcattagatt
      901 atggaaaaga tatttaggat tgattatttt cagaaaaatg aattgttaat tgttgtagca
      961 attatcattg gaatattagt ttttgcttct ttgttatttt atgtaattgg gcttttaaaa
     1021 cctgatgcta aattacaaga attaaaagca agaactaaat cttggtggat aatggcgagt
     1081 atatttattt tcgcgacaat tgtaaacgca aaaatatcat atgttgcgat tgcatttctt
     1141 tcatttgtag catttcgcga actttactca ttaatgggat ttcgagagtc agatagaaga
     1201 gcaatatttt gggcattttt atcaatacca attcaatatt atttagcatt tattggctgg
     1261 tatggtgctt ttataatttt cattccagtt gttatgtttt tattattgcc atttagattt
     1321 gttttaaaag gcgaaactga tggaataata aaatcaatgg cttctttgca atggttactc
     1381 atgcttacag tttttggtat tagccacctc gcatatttat tatcattacc tgaattgcca
     1441 aactttaatt cgggaggtag aggcttactt ttgtttgttg ttttcatgac agaaattaac
     1501 gatgtaatgc aatttgtatg gggtaaactg tttgggaaaa agaaaattat tcctaagatt
     1561 agtcccaata aaacatggga agggtttatc ggaggagtta ttagcacaac tattattgga
     1621 tattgcctag gttttttaac gccactaaat ggatatgaat tagtattcgt aagttttatg
     1681 attgctttca caggctttat tggagatgta gtgatgtcat cgataaaaag agatattggt
     1741 gtaaaagatt ctgggaattt aataccaggg catggaggtg ttttagatag aatagattca
     1801 cttatgtaca cagctccagt attttttcat ttagtttatt atatagcgta ctaatgaaaa
     1861 agcttatact acaaattgtt tataatgtaa ttattagaac ctttttaaga gttattattg
     1921 gggttaaatt cgaaaaccct aattttcttc tcactgagaa acagtttgtt ataatagcta
     1981 atcacaatag ccatttagat acaatggctg ttatggcatc tttaccgcgt aaaataattc
     2041 ataaagttaa gccagttgca gcaatggact attttgggaa aaccaaattg caagaaaagt
     2101 taagtaatta ttttataaat actcttttag ttaaacgaat aactgcgact gaaaaagggg
     2161 gtgagaatcc gataaggcaa atgataaagg aactcgacaa aggacactct ttaataattt
     2221 tccctgaagg ta
//
LOCUS       DAOPUR010000260         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_52268_length_2222_cov_2.825565, whole genome shotgun sequence.
ACCESSION   DAOPUR010000260 DAOPUR010000000
VERSION     DAOPUR010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52268_length_2222_cov_2.825565"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKN90_05150"
     CDS             <1..345
                     /locus_tag="PKN90_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNZ85698.1"
                     /translation="NDVAALLGKAGVNMTAFSLADNSDFGILRVIVSDPHKAQEVLKA
                     QKFAVSLTDVIHLQIANTPGSLSNVLAVLADAGMYIEYMYAFSEGNRANVIIKPDNVP
                     NAVEALQAHKIM"
     gene            406..1821
                     /locus_tag="PKN90_05155"
     CDS             406..1821
                     /locus_tag="PKN90_05155"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNZ85699.1"
                     /translation="MKKILLFFALMLLLGCTTKPPYVPPGGDIAGSSDYVFDITSLPE
                     ITITVPLEEWNLLLSNYDINRDNEEKVVAHFAFKKNGKVDEIANVGLRLRGNTSRKRP
                     EGYEGQPHNPINPDWRHAHFSVKFDEYVSKRTFYSIDHLILKWFKDDGMYCREIYCYD
                     LFKRFGVWTAPRASYAKLTINIEGGAEPAYFGVYAMIEGVNDSYLDYRKEQGKWASSK
                     GNLWKAKFSQADLSNMDDWRMGISNVTLDPAETVEYVYDLKTNKKTGLDAAKSELKDF
                     INNMNPLKSGSVALKSYLEEHMDVDLFLRAYAVNVMVGMWDDYWMSANNYYFYFDTNG
                     KFYFIPFDYDNTLGTSLYVNAGTHDMLNWGPRDGTRVLMRKVLSIQEYEDRFKEYIKE
                     LADANNDLFYTTKSIARIQSWHAMIEPYIANDTGQDMNLVDVPGSWGNCEFYRILSGN
                     DEGGENGDANFFKTKIKSIYW"
     gene            1891..>2222
                     /locus_tag="PKN90_05160"
     CDS             1891..>2222
                     /locus_tag="PKN90_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85700.1"
                     /translation="MKRCIMFIFYTSLLCTLFGQTKIHVHRSDAVDIRLFTSLLDSIN
                     FQALPEKPSEYSTLTLHKNNQTTQVVPLQAVDSIEFGVDQSEYIFDITALPEIKLTVK
                     LKDWNALLT"
BASE COUNT          739 a          325 c          435 g          723 t
ORIGIN      
        1 aatgatgtag cagccttgtt gggaaaagca ggtgttaata tgacagcatt tagtttagct
       61 gataattctg attttggtat tttgcgtgtt attgtatctg atccacacaa ggctcaggag
      121 gtactcaagg cacaaaaatt tgctgtttcg cttaccgatg ttattcattt acagatagca
      181 aatacgcctg gctcattatc caatgtactt gcagtattgg cagatgctgg tatgtatatt
      241 gagtatatgt atgctttttc cgaaggaaat cgagctaatg taataatcaa acccgataat
      301 gtgccgaatg ctgttgaagc attacaagca cataaaataa tgtaaaataa ttagacaata
      361 atctgtcttt ttatttattt tcgtttacag accttattct tatacatgaa aaaaatactt
      421 ctttttttcg ctctaatgct tttactagga tgtaccacaa aacctcccta tgtacctcct
      481 ggaggagata ttgctggaag tagtgattat gtatttgata ttacctcatt gcctgaaata
      541 acaataacgg ttcctttaga agaatggaat ttattgctat ctaattatga tattaaccgt
      601 gataatgagg aaaaggtagt agcacatttt gcattcaaaa aaaatggcaa agtagatgaa
      661 atagccaacg ttggtttacg tcttagagga aataccagta ggaaaagacc cgaagggtat
      721 gagggtcaac cacacaaccc aattaatcct gattggcgtc atgctcattt ctcagttaaa
      781 tttgacgaat atgttagtaa acgtacattt tatagcatcg accatttaat tttaaaatgg
      841 tttaaagatg atggcatgta ttgtcgtgag atttattgct acgatttatt taaacgtttt
      901 ggtgtatgga cggctccacg tgccagttat gcaaaactaa caatcaatat agaaggagga
      961 gcagaaccag cctattttgg ggtgtacgct atgattgaag gagtgaacga ttcttattta
     1021 gattatcgta aagaacaagg taaatgggca agtagtaaag gtaacttgtg gaaagcaaaa
     1081 ttttctcaag cagacctctc aaatatggat gattggcgta tgggtattag caatgttacg
     1141 cttgatcccg ctgaaactgt tgaatatgtg tatgatttaa aaacaaataa aaaaacagga
     1201 ttagatgctg ctaaatcaga attgaaagat tttatcaata acatgaatcc cttaaaaagt
     1261 ggcagtgtcg ctctaaaaag ttatttagaa gaacatatgg atgtagatct ttttttacgt
     1321 gcttatgccg taaatgtaat ggtcggtatg tgggatgatt attggatgag tgctaataac
     1381 tattattttt attttgatac gaacggcaaa ttttatttta ttccgtttga ttatgataat
     1441 accttaggaa catcattgta tgtaaatgca ggtacacatg atatgctaaa ttggggaccg
     1501 agagatggaa cgcgtgtatt gatgcgtaaa gtgttgtcaa tacaagaata tgaagatcgt
     1561 tttaaagagt acatcaaaga attggctgat gctaacaacg acctttttta tacaacaaaa
     1621 agtattgctc gtattcaatc gtggcatgct atgattgaac catatattgc caatgatact
     1681 ggacaagata tgaacttagt agatgtacct ggttcatggg ggaattgcga attttaccgt
     1741 attctttctg gaaatgatga aggaggagag aatggtgatg ctaatttctt taaaacaaag
     1801 attaaaagca tttattggta aatgagataa gtgttagtac tttcatatat aaaatggctg
     1861 aatatttcaa aaccttaaaa tctgattact atgaaaagat gtattatgtt tattttttac
     1921 acaagtttgc tttgtacgct ttttggtcag accaaaattc atgtacatcg ttctgatgct
     1981 gtggatatta gattgtttac ttcgctactt gatagcataa actttcaagc actaccagaa
     2041 aaaccctctg aatattcaac tttaacatta cataaaaaca accaaacaac acaagttgtt
     2101 ccattgcaag cagtagacag catagaattt ggtgttgacc aaagtgaata tatttttgat
     2161 ataacagctc ttccagagat taagttgacc gtgaaattga aagattggaa tgctttgctc
     2221 ac
//
LOCUS       DAOPUR010000261         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_52766_length_2209_cov_3.582637, whole genome shotgun sequence.
ACCESSION   DAOPUR010000261 DAOPUR010000000
VERSION     DAOPUR010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52766_length_2209_cov_3.582637"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PKN90_05165"
     CDS             <1..677
                     /locus_tag="PKN90_05165"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNZ85701.1"
                     /translation="LVMGSQETYYPALDTDRENGCIRSVDKAYTKDGGLAILKGNIAI
                     DGCVIKTAGVDESIWKFSGTAKVYQSQDDACYAILKGDVKAGDVVVITHEGPKGGPGM
                     QEMLYPTSYIKAKHLGKECALITDGRFSGGTSGLSIGHISPEAAAGGAIGLVKDGDII
                     DIDIPNRSINVRISDNELENRRKEEEGKGKEAWKPIRDRKISTALKAYASMVTSADKG
                     AVRVVE"
     gene            759..>2209
                     /gene="ilvB"
                     /locus_tag="PKN90_05170"
     CDS             759..>2209
                     /gene="ilvB"
                     /locus_tag="PKN90_05170"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNZ85702.1"
                     /translation="MEKKTMQGSYALLESLLNEGVDTIFGYPGGAIIPVFDAMYDYYD
                     KLRYILTRHEQGATHAAQGYARVSGKVGVCIVTSGPAATNAVTGISDAMLDSTPLVVI
                     TGQVSAALLGTDAFQEIDVVSITQPITKWSYQIRKADEIAWAVARAFYIAQSGRPGPV
                     VLDIAKNAQLENVDFIPQKCTFIRSYIPTCKLDDTVAKQAAKLINEAKKPFALVGQGV
                     TLGNAEDELLAFIEKANIPAAWTLLGESAMPTDHPLNKGMLGMHGNIGPNLKTNECDV
                     LIAIGMRFDDRVTGNLNEYAKQAKVIHFDIDPAEINKNVQTEVAVLGDVKTTLPAVTQ
                     YLEKNSHTEWIASFKEHEQKEYDFVIQKETESTEGAIKMGEVVLKVSKATNHSAILVS
                     DVGQNQMMAHRYFRFTQKRSNVTSGGLGTMGFGIPAAIGAKIGAPNRTVCLFCGDGGF
                     QMTIQELGTIMQEKIGVKMIILNNHFLGMVRQW"
BASE COUNT          765 a          368 c          459 g          617 t
ORIGIN      
        1 atttggttat gggttcgcaa gaaacatact atccagcttt agatactgat cgtgaaaatg
       61 gatgtatcag aagtgtagat aaagcctata caaaagatgg tggactagct attctcaaag
      121 gaaacattgc tattgacggc tgtgtaatta aaacagcagg agtagatgaa agtatttgga
      181 aattttcagg cacagccaaa gtatatcagt cgcaagacga tgcatgttat gctattttaa
      241 aaggagatgt aaaagcaggt gatgttgtag ttattacaca cgaaggaccc aaaggaggtc
      301 caggaatgca agaaatgcta taccctacct cctatatcaa ggctaaacac ttaggcaaag
      361 aatgtgctct gattacagat ggacgtttct cgggaggaac ttctggttta tctattggac
      421 atatttcgcc tgaagctgca gctggtggtg ctattggttt agtaaaagac ggcgatatta
      481 ttgatataga tattccaaat agaagtatca atgtacgtat ttcagataat gaactagaaa
      541 atagacgaaa agaagaagaa ggtaaaggta aagaagcttg gaaacctatc cgtgacagaa
      601 aaatttctac agcactaaaa gcctatgcaa gcatggtaac atctgctgat aagggggctg
      661 tacgagtcgt tgagtaatct ttactaagat actcttttat tctttttgtt tacaattata
      721 ctatttcaat tttattgatt ttatataaac tatataatat ggaaaaaaaa acaatgcaag
      781 gttcatacgc cttattggaa tcccttttaa acgaaggtgt tgatactatc tttggctacc
      841 ctggaggtgc cattattcca gtatttgacg ctatgtacga ttattacgat aagctacgtt
      901 atatcttaac tcgccacgaa caaggagcca cacatgcagc acaaggttat gcccgcgtat
      961 cgggtaaagt tggggtatgt attgtaacat ctggtccagc tgccaccaat gcagtaacag
     1021 gcattagtga tgctatgcta gatagtaccc ctttagttgt aattacagga caagtaagtg
     1081 ccgctttatt aggaacagat gcttttcaag aaatagatgt agtaagtatt acccaaccca
     1141 tcactaaatg gagttatcaa atacgcaaag cagatgaaat agcatgggca gtagcacgag
     1201 cattttatat tgcacaaagt ggacgaccag gacctgttgt attggatatt gctaaaaatg
     1261 cacaattgga aaacgtagat tttattccac aaaaatgtac atttattcgt agttatatac
     1321 ctacatgtaa acttgatgat acggtagcca aacaagctgc aaaattaatt aatgaagcaa
     1381 aaaaaccttt tgctttagtt ggtcaaggtg ttacgcttgg aaatgctgaa gatgaattac
     1441 tagcatttat agaaaaagca aacattcctg cggcatggac cttattggga gaatcagcaa
     1501 tgccaaccga ccatccttta aacaaaggta tgctgggaat gcacggaaat atcggaccaa
     1561 accttaaaac caacgaatgt gatgtattaa ttgctatcgg tatgcgtttt gacgaccgtg
     1621 taacagggaa tttaaatgaa tatgctaaac aggctaaagt tattcacttt gatattgatc
     1681 ctgctgaaat taacaaaaac gtacaaacag aagtagccgt tttaggagat gtaaaaacaa
     1741 cacttcctgc tgtaacacaa tatcttgaga aaaattctca tacagaatgg atagcatctt
     1801 ttaaggaaca tgaacaaaag gagtacgatt ttgtcattca aaaagaaaca gaatctaccg
     1861 agggtgctat caaaatgggt gaggttgttt taaaagtatc taaagctacc aaccatagtg
     1921 ctattttagt aagtgacgta ggtcaaaacc aaatgatggc acatcgttac ttccgtttta
     1981 cacaaaaacg gagcaatgtt acctcaggag gattaggaac aatgggcttc ggtatccccg
     2041 cagcaattgg tgcaaaaata ggtgccccta atcgtaccgt atgtttattc tgtggcgatg
     2101 gaggtttcca aatgacaata caagagttag gaactattat gcaagaaaaa attggcgtaa
     2161 aaatgattat tttaaacaat cactttttag gaatggtacg acaatggca
//
LOCUS       DAOPUR010000262         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_52951_length_2204_cov_2.743136, whole genome shotgun sequence.
ACCESSION   DAOPUR010000262 DAOPUR010000000
VERSION     DAOPUR010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52951_length_2204_cov_2.743136"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PKN90_05175"
     CDS             complement(<1..783)
                     /locus_tag="PKN90_05175"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNZ85703.1"
                     /translation="MNVADKLKQATSPLFTFELLPPLKGKGIDAIYKAIDPLLEYNPS
                     YINMTYHSAEVVYKTLADGKIEKRTVKKRPGSVALAAAIKYKYNIEVVPHVICSGFNQ
                     EETENILIDFHFLDIHNLLVLRGDPPKGMRVFQPKEDGHEHTLGLMSQINNMNNGRYL
                     DENLKNPEKTTFSMGVAGYPEKHMEAPNMVSDMKYLKQKVENGAEYIVSQMFFDTQKY
                     IDFVHQCRKEGILVPIVPGIKPIASMNDIKVLPQIFNIDLPEE"
     gene            complement(827..>2204)
                     /locus_tag="PKN90_05180"
     CDS             complement(827..>2204)
                     /locus_tag="PKN90_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNZ85704.1"
                     /translation="VGVVLAYNNFEVIDLGVMVPASEILRKAKEVNADIIGLSGLITP
                     SLEEMASVAEEMEKQGFTLPLLIGGATTSKLHTSVKIAPCYSHGVVHVIDASKTIPVA
                     KALCDRNKKEAFLAEIDIQHRETREKYASLKKVELISLAEARNNALQIDWKKEQIHVP
                     KITGLQIWKNYPIEELIPYIDWTYFFVAWQLKEKYPKILEDSPFKEEAIKLFADAQKI
                     LERMATEKLVVANGVFGIYPANTVEDTVEVYADENREKPIALFHFLRQQTKKKNDEKN
                     LSLADYIPSKKSGIGGYIGGFAITAGIHLTELTDSYEKEGDDYSAIMCKILSDRLAEA
                     FAEKLHADIRTTYWGYETIHATPQEMFEGKYQGIRPAIGYPSIPDHSEKETLFNLLQV
                     TEHTGITLTENYAMFPNASVCGLYFDTARASYFNIGRIGEDQVNDYANRKQIDITFLK
                     KLIAKNLE"
BASE COUNT          579 a          420 c          409 g          796 t
ORIGIN      
        1 ttcttcgggt aagtcgatat taaaaatttg tggtaatact ttgatatcgt tcatgctagc
       61 aatgggttta atgcctggta ctatcggcac gaggatacct tctttgcgac attgatgtac
      121 aaaatcaatg tatttttgtg tatcaaaaaa catttgcgac acaatatatt cagcaccatt
      181 ctctactttt tgttttaagt atttcatgtc agataccata ttgggagctt ccatgtgttt
      241 ttcaggatat cctgctacac ccatcgagaa agtggttttt tcaggatttt ttaaattttc
      301 atccaaatac cgtccattat tcatgttgtt aatttgcgac atcaacccta aggtatgctc
      361 gtgtccgtct tcttttggtt gaaatacacg catgcctttt ggtgggtcgc ctctaagtac
      421 caataaatta tgaatatcca aaaaatgaaa atcaatcaat atattttctg tttcttcttg
      481 attaaaaccg ctacaaataa cgtgaggcac tacctcaata ttgtatttat atttgatagc
      541 agcagctaat gctacgcttc cagggcgttt ttttacggtt cttttttcaa tcttaccatc
      601 cgccaatgtt ttatatacta cttcggcact gtggtaggtc atattgatat acgaaggatt
      661 gtattctaaa agagggtcta tcgctttgta tatggcatca attccttttc ctttaagggg
      721 aggtaatagt tcaaaagtaa aaaggggcga tgttgcttgt ttgagtttat ctgctacgtt
      781 catgttgtat tttttcgtta agccaacaaa attacgaaaa cttattttat tctaaatttt
      841 ttgctatgag tttttttaaa aaagtgatat caatttgttt ccgattggcg taatcgttta
      901 cttggtcttc tccaattcgt cctatattga agtacgatgc tctggcagta tcaaaataca
      961 aaccgcaaac acttgcattc ggaaacatgg cgtaattctc tgtcaaggta atgcctgtat
     1021 gttcagttac ttgtagcaag ttgaataagg tttctttttc agagtggtcg ggaattgatg
     1081 ggtagccgat ggcgggtcga atgccttggt acttgccttc aaacatttct tgtggtgttg
     1141 cgtgtattgt ttcgtaaccc cagtaggttg tacgtatatc agcatgtaat ttttcggcaa
     1201 atgcttcggc taatctatcg ctcaatattt tgcacataat ggcagaatag tcgtcgcctt
     1261 ctttttcgta actatctgtt aattctgtaa gatgtatgcc cgcagtaata gcaaaaccgc
     1321 caatataacc gcctatccct gatttcttag aaggaatata atcggcaagc gaaaggtttt
     1381 tttcgtcgtt cttcttcttt gtttgttgac gcaagaaatg aaacaatgcg attggttttt
     1441 cgcgattctc atcagcatat acttctacgg tatcctctac cgtattggct ggataaatgc
     1501 caaatactcc attggcgact accaattttt cagttgccat acgctcaagt atcttttggg
     1561 catcagcaaa tagcttaata gcttcttctt taaaagggct atcttctagt attttcgggt
     1621 atttttcttt taactgccat gccacaaaaa agtatgtcca atcaatatat ggaataagct
     1681 cttcaattgg gtaatttttc caaatttgca agcctgttat tttgggaaca tgaatctgtt
     1741 cttttttcca atctatttgt agggcattgt tacgtgcttc agccaatgaa ataagttcta
     1801 cttttttcaa cgaggcatat ttctctctgg tttctcggtg ttgtatatca atttcagcta
     1861 agaaagcctc ttttttgttc ctgtcgcaca atgctttagc aactggaatg gtttttgatg
     1921 cgtctattac atgaactaca ccgtggctat aacaaggagc tattttcacg cttgtatgca
     1981 acttggaggt agtagctcct ccaatgagca gaggcaaggt aaaaccttgt ttttccattt
     2041 cttctgctac cgatgccatt tcttcgagcg agggggttat taagccactc aatccgatga
     2101 tatcagcgtt tacttctttg gctttgcgta atatttctga tgcaggcacc attacaccaa
     2161 ggtcgataac ttcaaaatta ttgtaggcaa gtactactcc taca
//
LOCUS       DAOPUR010000263         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_53568_length_2188_cov_2.189405, whole genome shotgun sequence.
ACCESSION   DAOPUR010000263 DAOPUR010000000
VERSION     DAOPUR010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53568_length_2188_cov_2.189405"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="PKN90_05185"
     CDS             <1..169
                     /locus_tag="PKN90_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85705.1"
                     /translation="QLKQTTMDPENRTLRQVTIENAAEADHIFSMLMGDDVAPRRDFI
                     EKNATYARIDA"
     gene            184..729
                     /locus_tag="PKN90_05190"
     CDS             184..729
                     /locus_tag="PKN90_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNZ85706.1"
                     /translation="MNVCVFCSSSNEIAEEYREDARLLGEVIGKKSYTLVYGGANMGL
                     MQECAMAVKRSGGSVIGVIPDSFRKKVEQKVLDQVFFVGSLAERKELMMEYADVFVAL
                     PGGIGTLDEVFEVLALAMIDNHHKKVLFLNTHGYYTHLVAHIEHSIQEKCVHPAMKNS
                     YIVVNTVDECMAYIDKENVVK"
     gene            726..1562
                     /gene="kdsA"
                     /locus_tag="PKN90_05195"
     CDS             726..1562
                     /gene="kdsA"
                     /locus_tag="PKN90_05195"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNZ85707.1"
                     /translation="MIIPSTQQVVIPNHFVIGGTSRFVFIGGPCAIESEQMSIDVAGS
                     LKEICDKLGIHFVFKSSFDKANRSSVFSPRGVGMDKGLSILQKVTDTYGIPILTDVHE
                     SWQCDAVGSVVDVIQIPAFLARQTDLLVAAAKTGKVVNIKKAQFMAPWDMQQVVNKMI
                     ETGNDSIVLTERGSSFGYNNSVVDMTSLIEMRKIGYPIVFDATHAVQKPGGNGINSGG
                     NRSYVAPLMRAALAVGVDAIFAEVHPNPDMAFSDAANQLHLDSIHTILEQAVTIDDII
                     KK"
     gene            1582..>2188
                     /locus_tag="PKN90_05200"
     CDS             1582..>2188
                     /locus_tag="PKN90_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNZ85708.1"
                     /translation="MMYAERSKEVFRQEIQALEKVASRIGAEMDKVVELLFASTGKIV
                     VTGIGKTGIIAHKIASSLASTGTPAFFMNAAEAMHGDLGMISANDVVLIVSNSGASQE
                     ILRIIPPIKHINCPIIALTGNVSSPLANEADYVLDVSVDKEACPLGLAPTTSTTATLV
                     MGDALIVSLMEKRHFKADDFALNHPGGALGKRLLVKVKEIMQ"
BASE COUNT          709 a          321 c          498 g          660 t
ORIGIN      
        1 gcaattgaaa caaacaacaa tggacccaga aaatagaaca ttgcgacaag taaccataga
       61 aaatgcagct gaagcagatc atattttttc tatgttaatg ggtgatgatg ttgctccaag
      121 acgtgatttt atagaaaaaa atgctaccta tgcacgcatt gatgcgtaaa aaatggaatt
      181 gtgatgaatg tgtgtgtctt ttgttcttca agcaatgaaa tagccgaaga gtatcgtgaa
      241 gatgctcgtt tgctaggaga agtaattggt aaaaaatcat atactctggt atatggagga
      301 gccaatatgg gtttgatgca agaatgtgcg atggcggtaa aacgatcagg aggtagtgtg
      361 attggcgtta ttccagattc ttttagaaaa aaagtagagc aaaaagttct tgatcaagtc
      421 ttttttgttg gtagtttggc tgaaagaaaa gaactaatga tggaatatgc agatgtcttt
      481 gtggctttgc ctggggggat tggtacatta gatgaggtat ttgaagttct tgccttagca
      541 atgatagata accatcacaa gaaagtattg tttttaaata cccatggcta ctatacacat
      601 ttagttgctc atatagaaca cagtatacaa gagaaatgtg tgcatcctgc gatgaaaaac
      661 tcgtatattg tagttaatac agtagacgaa tgtatggcat acattgataa agaaaatgtt
      721 gtaaaatgat tattccaagt acacagcaag tagtaatccc caatcatttt gttattggag
      781 gaactagtcg ttttgttttt attggtggac cctgtgccat agaaagcgaa caaatgtcaa
      841 tagatgtggc aggcagtttg aaagagattt gtgataaatt aggtatacat tttgttttta
      901 aatcatcttt tgataaagca aatcgctctt ctgtgttttc tccccgtggt gtaggaatgg
      961 acaaagggtt atcaatttta caaaaggtta cagatacata tggtatacct atacttacag
     1021 atgtacacga aagttggcaa tgtgacgcag tgggttccgt agtagatgtt atacaaatac
     1081 ctgctttttt agctaggcaa accgatttat tagttgcagc agctaaaacg ggcaaagtgg
     1141 tgaatataaa aaaagcccaa tttatggctc cgtgggatat gcaacaagtg gtaaataaaa
     1201 tgatagaaac gggaaatgat tctattgtac taacagaaag aggatccagt tttgggtata
     1261 ataattcggt agtagatatg acatcattaa tagaaatgcg taaaataggt tatcctatcg
     1321 tttttgatgc tacgcacgct gtgcaaaaac ctggtggaaa tggaataaat tcaggaggca
     1381 atcgctccta tgttgctcca ttaatgcgag cagcattggc agtaggtgtt gatgcgattt
     1441 ttgctgaagt acatcctaat ccagatatgg ctttttcgga tgcggccaac caattgcact
     1501 tggatagtat ccatacaata cttgagcagg cggtaacaat agatgatatt ataaaaaaat
     1561 agtagactaa tacccgatcg tatgatgtac gctgaacgta gcaaagaagt ttttaggcag
     1621 gagatacaag ctttagaaaa ggtagcatca cgcataggtg cagaaatgga taaagtagtt
     1681 gagttattgt ttgctagtac aggtaagatt gtggtaacgg gtattggtaa aacgggtatt
     1741 attgcacata aaattgcctc ttcgttggct tctacaggaa ctcctgcttt ttttatgaat
     1801 gcggcagaag caatgcatgg agatttaggt atgatatccg ccaatgatgt agtgttgatt
     1861 gtatctaaca gtggagcttc gcaagaaata ttgagaatca tacctcctat taagcatatc
     1921 aattgtccga ttatagcatt aacaggtaat gtttcttctc cacttgctaa cgaggctgat
     1981 tatgttctcg atgtgagtgt tgataaggaa gcttgtccgt tggggttagc tccaactact
     2041 tctaccacag ccacattagt aatgggagat gctttaattg ttagtttgat ggaaaaacga
     2101 cactttaaag ccgatgattt tgctcttaat catcctggag gtgcattggg gaaacgatta
     2161 ctagtaaaag taaaagagat tatgcaaa
//
LOCUS       DAOPUR010000264         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_53709_length_2184_cov_2.999061, whole genome shotgun sequence.
ACCESSION   DAOPUR010000264 DAOPUR010000000
VERSION     DAOPUR010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53709_length_2184_cov_2.999061"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PKN90_05205"
     CDS             complement(<1..1251)
                     /locus_tag="PKN90_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85709.1"
                     /translation="MNTSRTSTSFLYPLLSWNFVLRLLFIGLFLVVSLANTFAIKVRN
                     DNAWASVRIHYWNGGTGSDWGSRPTMTATTTPAWDTSGKSWVEWNINTCTDIKLTGTN
                     EPPSTNDINGVSNDGCYTMSSDATPTLTRVDCATGGAYVPPVPVINFAVTGGVVSGTT
                     QWNTSRPSSCIFEQTGTNTYSLCFIYDSKAFKLLRKENGLPNTNWDWSSAVAIVAGSN
                     VSGSSGDLTITGFTTGDPVCLQLVVSGTTYTLSAEQSVHITSNLPNPINQGADITLTA
                     NNVSSACTWEYSTDGGTTWQPYTGATRGTYNEEISPSPLTNMTYRVTSDGKTATYMVQ
                     VVINCQGSIQTHFTETFGSLPTATARTTSADINSLVYQYSPEGKEVHDGWYAVVANPR
                     NCGRGAAQSTFSGGVVTVLGDVRTS"
     gene            complement(1365..>2184)
                     /locus_tag="PKN90_05210"
     CDS             complement(1365..>2184)
                     /locus_tag="PKN90_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ85710.1"
                     /translation="SEKPFPEENKPIYSVVSYNIHHFKFFPKEKETKTGNHILDYLVE
                     LDADIICLQEFAYSDYEPFTLSDIKNQLSDYPYNHIEILYKDRHISKGIATFSKHKIT
                     NKQHISIPAEYHGAISSDVLLDGKKIRIINFHFESNRLTSKDKDIKRLTDRNKIPSFA
                     KRLQSKLHTASRKRGKQADALMTFATNSNMPTIICGDMNDVPSSYTYRTITQHYTDNF
                     KQVGKGFGNTFHENFYRYRIDYTFTNKQINPLKIKTHPYDISDHFPLQLDFQTP"
BASE COUNT          644 a          386 c          436 g          718 t
ORIGIN      
        1 gctggtacgt acatctccta atacagttac aactcctcca gaaaaagttg attgagcagc
       61 acctctaccg caattacgag ggttagccac tacggcatac caaccatcgt gtacttcttt
      121 tccctcaggg ctatattgat aaactaagga atttatatca gcagaagttg ttcgtgcagt
      181 agctgtaggc aaagagccaa aggtttctgt aaaatgagtt tgaatacttc cttggcagtt
      241 aataacaact tgtaccatat acgtagctgt cttcccatca gatgtaactc tatacgtcat
      301 attggtaaga ggacttggag atatttcttc attgtatgtg cccctggttg ctccagtata
      361 aggttgccat gttgtaccac catcggttga atattcccat gtacaagcag aagaaacatt
      421 attcgccgtt aatgtaatat ctgctccttg gtttattgga ttaggaaggt tagatgtaat
      481 atgtacactt tgctctgctg ataatgtgta ggtagttccc gatacgacta gttgtaagca
      541 gacaggatct cctgtagtaa acccagtaat agttaaatct ccagaactac cactcacatt
      601 acttcctgct acaatcgcaa ctgcacttga ccaatcccaa ttcgtatttg gcagtccgtt
      661 ttcttttcgt aacagtttaa atgctttgga gtcgtaaata aagcaaagag aataggtatt
      721 ggttcctgtt tgctcaaaaa tacatgatga gggacgagat gtgttccatt gggtagtgcc
      781 acttactaca ccaccagtta ctgcaaaatt tattacaggt acaggcggta catacgctcc
      841 accagtagca caatcaactc gtgttaatgt aggggtagca tcagaactca tggtgtagca
      901 accatcatta cttacgccat tgatgtcatt tgtactggga ggttcatttg ttccagttag
      961 tttgatgtct gtacacgtat taatattcca ttccacccag cttttcccac ttgtatccca
     1021 agcaggtgta gtggtagctg tcatcgttgg gcgagaaccc caatcagaac cagttcctcc
     1081 gttccaataa tgtatgcgga cggaagccca ggcgttgtcg tttcttactt ttattgcaaa
     1141 ggtatttgca agactgacta ctaaaaatag tccaataaat aataggcgta atacaaaatt
     1201 ccacgagagg agtgggtaca aaaaggacgt gctagtacgt gatgtattca tactatgttg
     1261 aattaaggtt agcgtaacag tatatgcccc aaagttaaaa aaaataaatg aaatacaaaa
     1321 cttcttgaaa gaaaaagttt atcgtttgaa tagtagcgag ttaactaagg tgtctgaaag
     1381 tctagttgta aggggaaatg gtcagaaata tcataaggat gagtttttat ttttaatggg
     1441 ttgatttgct tatttgtaaa agtataatca atacgatatc ggtaaaagtt ttcgtgaaac
     1501 gtattgccaa aacctttgcc tacttgttta aaattgtctg tataatgttg agtaatcgtt
     1561 ctatatgtat acgaagaagg gacatcattc atgtccccac atataatagt aggcatgtta
     1621 gagtttgttg cgaatgtcat taatgcatct gcttgtttgc ctcttttgcg tgacgcagta
     1681 tgaagtttcg attgcaatct tttagcaaac gatggtattt tatttcgatc agttaagcgt
     1741 tttatatctt tgtctttgct ggttaatctg ttcgattcaa agtgaaaatt aataatgcgg
     1801 atttttttgc catctagcag aacatcagag gaaatggctc catgatattc tgcagggata
     1861 gaaatgtgtt gtttattcgt tattttgtgt tttgaaaaag tggcaatacc ttttgagata
     1921 tgtctgtctt tatataatat ttctatatga ttgtatggat aatcagacag ttgattcttt
     1981 atgtcagata atgtaaacgg ctcgtaatca ctatatgcaa attcttgcaa gcagattata
     2041 tcggcatcaa gttctaccaa ataatccaga atatgattgc ctgttttcgt ttctttttct
     2101 ttagggaaaa atttaaaatg gtgtatattg tacgaaacca cggagtatat aggtttattt
     2161 tcctctggaa aaggcttttc agac
//
LOCUS       DAOPUR010000265         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_54000_length_2175_cov_3.018868, whole genome shotgun sequence.
ACCESSION   DAOPUR010000265 DAOPUR010000000
VERSION     DAOPUR010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54000_length_2175_cov_3.018868"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKN90_05215"
     CDS             <1..406
                     /locus_tag="PKN90_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ85711.1"
                     /translation="FNLIHFKNALENVALPLYYKGIARKKRNAIALEYLEKMGLKDWA
                     HHYPNELSGGQKQRVAIARALISNPRVILADEPTGALDSQTSVEVIDLLKAINQQGVT
                     VIVVTHENEVAASTERIIRMKDGIIQPQDIHQ"
     gene            410..1675
                     /locus_tag="PKN90_05220"
     CDS             410..1675
                     /locus_tag="PKN90_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ85712.1"
                     /translation="MIHFQLWEEIFASLRRNKLKTALTGFAVAWGIFMLIVLLAAGNG
                     LKNGMALNYKNDSKLAVWMWSGRTNLAYKGLPTKRNIQFTNTNETILRKDFDASIGEL
                     SPLIDMRGKNVMYKNEKGIHDVRGVLPEFVPIEALEMLPNQGRFINQLDCDHKRKVVV
                     VSSKFVQDFFKDESPLGKQLSIDGIMFTVIGVFKEVPRSWQKLVYIPLPTAQSLYGFG
                     DKIHEISFNLHMQNETNVDESKQIMDNIKQRFASYHQFDVEDEGAMGYWNTLESFLFT
                     QKLFAGISIFVWIIGVGTLIAGIVGISNIMLISVKERTKEFGIRRALGAKPSSIIVLV
                     VLESIIITAFFGYIGMVFGVGLTEGVNYVMKQMPMDETGAVFFYNPTIQLDLVMVATV
                     LLIIAGVIAGYFPARKAIRIKPIDALRDE"
     gene            1680..>2175
                     /locus_tag="PKN90_05225"
     CDS             1680..>2175
                     /locus_tag="PKN90_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ85713.1"
                     /translation="MRIVDIDRWQEIWHTIKQNKLRSVMTAFGVFWGILMLVIMLGSG
                     KALENGILDGTENFERNSCFMFAGTTTEPYKGFQRGRSWYITSSDLVAIEKNVEGVKF
                     TVPYVFPTNTTENNVMNGMKVGSFSVQGITPELYLVNPQILLQGHLINKLDELGKRKI
                     CVIGE"
BASE COUNT          679 a          288 c          501 g          707 t
ORIGIN      
        1 atttaatcta attcatttta aaaatgcatt agagaatgtt gctttaccac tttactacaa
       61 agggatagct cggaaaaagc gtaatgctat agcattggag tatttggaaa aaatgggttt
      121 gaaagattgg gctcatcatt atcctaatga gctttctggg ggacaaaaac agcgtgtagc
      181 cattgctcgt gcacttattt ctaatccaag agtaatatta gctgacgaac cgactggagc
      241 cttggatagt cagacttcag tagaagttat agacttatta aaggcaatta accaacaggg
      301 tgtaaccgtg atagtggtaa ctcacgaaaa tgaagtagct gcctcaacag aacgtattat
      361 acggatgaaa gatggtatta ttcagcctca agatatacat cagtaagata tgatacactt
      421 tcaattatgg gaagaaattt ttgcgtcttt gcgtagaaat aaacttaaaa cagctttaac
      481 tggatttgct gtggcatggg gtatatttat gctcattgtt ttgctggcag caggtaatgg
      541 cttaaagaat ggaatggcat tgaattataa aaatgactcc aagttggctg tttggatgtg
      601 gtctggtaga actaatttgg catacaaagg gcttcctaca aaacgcaata ttcagtttac
      661 gaatacgaac gaaacaatat tgaggaaaga ttttgatgca tctattggag aattatcgcc
      721 cctgattgat atgcgaggga aaaatgtgat gtacaaaaat gaaaaaggga ttcacgatgt
      781 gcgtggtgtt ttgcccgagt ttgtgcctat tgaagcattg gagatgctcc ccaatcaagg
      841 acgttttatt aaccaattgg attgtgatca taaacgaaaa gtagtggttg taagttctaa
      901 atttgttcag gattttttta aagacgagtc cccgttaggt aaacaattga gtatagatgg
      961 tatcatgttt accgttatag gcgtttttaa agaagtgcct cgtagctggc aaaaattagt
     1021 gtatattcca cttcctacag ctcaaagttt gtatggattt ggagataaga tacatgaaat
     1081 atcgtttaat ttacatatgc aaaacgaaac caatgttgat gaaagcaagc aaattatgga
     1141 taatattaag caacgttttg catcatacca tcaatttgat gtagaagatg aaggagcgat
     1201 ggggtattgg aataccctag agagtttttt gtttacccaa aagttatttg ctgggatatc
     1261 tatttttgtg tggattatcg gtgttggaac tcttattgcg ggaattgttg gtattagtaa
     1321 tatcatgtta ataagcgtaa aagagagaac aaaagaattt ggtattcgac gagctttagg
     1381 agctaaacca tcttcaatta ttgtgttagt tgtgttagaa tcgattatta taacggcttt
     1441 ttttggttat atcggaatgg tgtttggtgt tggtctgacc gagggagtaa attatgtgat
     1501 gaagcaaatg ccaatggatg aaacaggtgc agtctttttt tataacccca ctattcaact
     1561 tgatttagtt atggtggcta ctgttttgct tattattgca ggtgttattg caggttattt
     1621 tcctgcacgt aaggctatta gaattaaacc tatagatgct ttgagagacg aataagtata
     1681 tgcgtatagt tgatattgac agatggcaag aaatttggca tactataaag caaaataagc
     1741 ttcgtagtgt aatgactgcg tttggtgtgt tttgggggat actgatgttg gttattatgc
     1801 ttggttctgg caaggctttg gagaatggga tattagatgg aactgaaaat tttgaaagaa
     1861 attcatgttt tatgtttgct gggactacta ctgaaccata taaggggttt cagcgtggac
     1921 gttcgtggta tattacatct agtgatttgg tggcgattga aaaaaatgta gagggagtta
     1981 aatttactgt cccttacgtt tttccaacaa atactacgga gaataatgtg atgaatggta
     2041 tgaaagtggg atctttctct gtacagggaa ttactccgga attgtatttg gtgaatcctc
     2101 agattttgtt gcaaggacat ctcattaata aattagatga actaggtaaa cgaaaaattt
     2161 gtgttatagg ggaaa
//
LOCUS       DAOPUR010000266         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_54035_length_2174_cov_3.572912, whole genome shotgun sequence.
ACCESSION   DAOPUR010000266 DAOPUR010000000
VERSION     DAOPUR010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54035_length_2174_cov_3.572912"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PKN90_05230"
     CDS             <1..952
                     /locus_tag="PKN90_05230"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNZ85714.1"
                     /translation="SLLQIESLVDLTGGMGIDSYFFSQRVKKLVYIEQQSNLCSIASH
                     NFNVLGAQNIDVINTQAEVYSNTMPMVDCVYIDPARRNITGAKVVLLEDCSPNVEAIL
                     PHILTKTRYVLLKLSPMFDIEMAVRSLPNIKEIHIVAVDNDCKELLVVLDVLHSLPSC
                     TYHCINIAENNQNQQHSYTNELVQGVHIHYTSQLSTYVYEPNVTILKAGCFSSIATAF
                     CVEKLHPNSHLFTSNQLHIDFPGRIFEVIEVVEFNKIAIKTLLTPIKKANVTVRNFPL
                     RVDEFRKKTQLKEGGDTYIFATTVANNKKVCIICRKINQK"
     gene            complement(939..1955)
                     /gene="murB"
                     /locus_tag="PKN90_05235"
     CDS             complement(939..1955)
                     /gene="murB"
                     /locus_tag="PKN90_05235"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNZ85715.1"
                     /translation="MRTLENFSLLNHNTFGVDVTANYFVEYDNITELQQLLGSDLLKE
                     NKFFHIGGGSNLLFINDYEGVILHSQIKGKEISKESADHVWIRVGAGENWDDFVDYCV
                     ENQLGGAENLSLIPGEVGATPIQNIGAYGVEVKDIIQSVDCIEISTGKQKTFSNKECA
                     FGYRDSIFKQGFRNKYVVCYVTYKLAKHYTPNVSYGSLSTEILNYKEVNIQTVRQAVI
                     AIRKRKLPEPKVLGNAGSFFMNPIIAMEHFLQLQQLYPDIPSFHVSDKHIKVPAGWLI
                     EQCGWKGKSIGKVAVHGSQALVLINKGGANGLDIIYLSDKIRESVKEKFNIDIIPEVN
                     IIFG"
BASE COUNT          735 a          372 c          334 g          733 t
ORIGIN      
        1 ctctttgcta caaatagaat ctcttgttga tttaacaggt gggatgggca tagatagtta
       61 tttcttttcg caacgagtga aaaaattagt gtatattgaa caacaaagta acttatgtag
      121 catagcatct cataatttta acgtattggg tgcacagaat attgatgtta taaatacaca
      181 agctgaagta tatagtaaca ctatgccaat ggtcgattgt gtgtatatcg accctgcacg
      241 caggaatatt acgggagcta aagtcgtatt gttagaagat tgtagcccta atgtggaggc
      301 tatattacct cacatactta caaaaacaag gtatgtatta ctcaaattat cacctatgtt
      361 tgatatagaa atggcggtga gaagtttgcc aaatataaag gaaatacaca tagtagcagt
      421 tgataatgat tgtaaagaat tgcttgttgt gcttgatgtt cttcattcgt tacctagttg
      481 tacgtatcac tgtatcaata ttgctgaaaa caaccaaaat caacaacata gttatacaaa
      541 tgagcttgta caaggcgtac acattcatta tacatctcaa ctttcgacct atgtatacga
      601 accaaatgtt accattttaa aagcaggttg ttttagtagt attgcaactg cattttgtgt
      661 agaaaagctt catcccaata gtcatttatt tacttcaaat caattgcata ttgattttcc
      721 gggtagaata tttgaggtaa tagaagtggt agaatttaat aaaatagcta tcaaaacatt
      781 actaacaccc attaaaaaag caaacgtaac ggtgcgaaat tttcctttac gagtggatga
      841 attccgtaaa aaaacacaat taaaagaggg tggtgataca tatatatttg ctacaacggt
      901 agcaaataat aaaaaagtat gtattatctg tcgaaaaatt aaccaaaaat aatgtttact
      961 tcaggtataa tgtcaatatt aaatttttct tttacagatt ctcttatttt atccgaaaga
     1021 taaataatgt ctaatccatt tgctccacct ttgttgatta gtaccaatgc ttggctaccg
     1081 tgtactgcta ctttgccaat tgattttcct ttccatccac attgttcaat taaccaacct
     1141 gcaggaactt taatatgctt atcagataca tgaaaacttg ggatatctgg atacaactgt
     1201 tgtaattgaa gaaaatgttc cattgctatg atgggattca taaaaaaact accagcatta
     1261 ccaagtacct taggttcagg gagttttcgt tttcggatgg caattacggc ttgtctaacc
     1321 gtttgtatgt tcacttcttt ataattcaag atttcagtag ataaacttcc ataacttaca
     1381 ttgggggtat aatgtttagc taatttgtac gtaacatagc aaacaacata tttattacgg
     1441 aaaccctgtt tgaatatact atccctgtat ccaaaagcac actctttatt tgaaaaggtt
     1501 ttttgtttcc cagtgctaat ttcgatacag tctactgatt gaataatatc ttttacttca
     1561 acaccataag ccccaatatt ttgaattgga gtagcaccta cctcgcctgg aattaaagat
     1621 aaattttcag cacctcctaa ttgattttct acgcaataat ctacaaaatc atcccaattt
     1681 tctcctgcac ctacacgtat ccatacgtgg tcagctgatt ccttgcttat ctcttttccc
     1741 tttatttgcg aatgtaaaat aaccccttca tagtcgttga taaagagcag attactgcca
     1801 cctccaatat gaaaaaattt gttctctttt aacaaatcac ttcctaataa ttgttgcagt
     1861 tcagtaatgt tatcatactc cacaaaataa tttgccgtaa catcgactcc aaatgtatta
     1921 tgattaagta gtgaaaaatt ttccaatgtt ctcatataat agcggatatt gtattagtat
     1981 tactaaaatt gtataaatca attttcggtt ttttataata attgtccatc tttgtttgtt
     2041 gtacaaagat ataaaattta tacttctaag agctacaaat agtaaaaaaa acgtactttt
     2101 gtaacatgag aaaaaagcaa atcatacttt cagttaccaa cgatttggaa acagaccaac
     2161 gtgtacataa ggta
//
LOCUS       DAOPUR010000267         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_54272_length_2168_cov_2.901088, whole genome shotgun sequence.
ACCESSION   DAOPUR010000267 DAOPUR010000000
VERSION     DAOPUR010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54272_length_2168_cov_2.901088"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKN90_05240"
     CDS             <1..494
                     /locus_tag="PKN90_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85716.1"
                     /translation="THIIFTGGEPTLYALDKLVIPGKKYHVETNGTVIPTEPLHIELP
                     DGTYIERVAMKESIIKRFNWVVSPKLSNAINSQNTEYLHYWSARNWGIFKFIIQQSAD
                     IDEIEHVINRYSIDKNRVYLAVEGCTLQSQLRPELVDELITKGFHYSPRLHVLLWGAK
                     RKK"
     gene            505..927
                     /locus_tag="PKN90_05245"
     CDS             505..927
                     /locus_tag="PKN90_05245"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNZ85717.1"
                     /translation="MQYVITKEFTFEAAHRLLRNYCGKCSNNHGHSYRVKLHLQGTNL
                     DEKDMLIDFNETKKLKAWIDDNLDHVTMLWEEDPMIDSMRTFGNKVLVTKKNPTAEHI
                     AELILAEAQRLFENSAIQVKCIEINETCTSAARLYPLS"
     gene            940..1320
                     /gene="queF"
                     /locus_tag="PKN90_05250"
     CDS             940..1320
                     /gene="queF"
                     /locus_tag="PKN90_05250"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNZ85718.1"
                     /translation="MNTPQFTHLGKKSNTPTKQLDTFPTPNNVEIVKFESDELTSFCP
                     VTHQPDFNRVIIEFSPDAYCIESKSLKLYLWSFREEAKFAEALAGEIADDIFNAIQPH
                     WCTITLIQNIRGGMQLSVVAKREK"
     gene            complement(1328..>2168)
                     /locus_tag="PKN90_05255"
     CDS             complement(1328..>2168)
                     /locus_tag="PKN90_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ85719.1"
                     /translation="FFFEVSIHGLQGGHSGDDIEKKRANANQLLNTYLLSIADKYSLW
                     LGDVRGGSLRNAIPREAYAWCAVPRKFKESVRVDWNIFIADVEQEWLAYEPSMRFVLS
                     TTDAFAEGWSKDALADFLQAVSACPHGVINWSEKMPTVVQTSTNLAVISSAHNGITVA
                     TNQRSLSEQERDEIAVRIRQLFELCGAEVLNQNHYPGWEPRFEAAFLKHATDVYTTLF
                     GQEPKVRVIHAGLECGVISGTYPAMDIISIGPTIKDAHSPNERVSISSVQKIWEYLLA
                     LLR"
BASE COUNT          726 a          468 c          390 g          584 t
ORIGIN      
        1 ctacacacat tatttttaca ggaggtgaac ctaccttgta tgcattggat aagttagtaa
       61 tacctggtaa aaaataccat gtagaaacga atggaacggt tatccccaca gagcctctgc
      121 atatcgaatt gcccgatggc acatacatag agagagtcgc tatgaaagaa tccatcatca
      181 aacgctttaa ttgggtagtt tcgccaaaac taagcaatgc gattaatagc caaaacacgg
      241 aatatttgca ctattggtcg gctcgtaact ggggcatatt caaatttatc atacagcaat
      301 ctgcagatat tgacgaaata gaacacgtaa ttaatcgtta ttctattgat aaaaaccgcg
      361 tatatctagc agtagaagga tgcacgttgc aatcgcaatt gcgacctgaa ttggtggatg
      421 aattgataac aaaaggtttt cactattcgc cacgcttaca tgtactattg tggggagcca
      481 aacgaaaaaa ataattatac aactatgcaa tacgttatca ctaaagaatt tacatttgaa
      541 gcagctcatc gtttactacg caattattgt ggcaagtgta gtaataatca cggtcattcg
      601 tatcgcgtaa aattgcactt acaaggtact aacttggatg aaaaagacat gcttatcgat
      661 tttaacgaaa caaaaaaact aaaagcatgg atagacgaca atcttgacca cgtaaccatg
      721 ctctgggaag aagatccgat gattgatagt atgcgtacgt ttggcaacaa agtactagtt
      781 accaaaaaga atcctacggc tgagcatatt gccgaactca ttttagcaga agcacaacga
      841 ctctttgaga attctgccat acaagtaaaa tgtattgaaa taaatgaaac ctgtacatct
      901 gcggcacgtt tatacccttt atcttaaaag aatgacacca tgaatacacc tcaatttaca
      961 catttaggca aaaaaagcaa cacacctacc aaacaactcg atacgtttcc aacacccaac
     1021 aacgtagaaa ttgtaaaatt tgaaagcgat gaattaacca gtttttgtcc tgtaacacat
     1081 caaccagatt ttaatcgtgt tatcatcgaa ttttctcccg atgcctactg tatcgaaagt
     1141 aaaagtttga aactctattt gtggtcattt cgcgaagaag ccaaatttgc cgaagcatta
     1201 gcaggcgaaa ttgcagacga tattttcaat gctatacaac cacattggtg tacaattaca
     1261 ctcattcaga acatacgagg aggtatgcaa ttgtctgtgg ttgctaaacg ggagaaataa
     1321 agttctgtta tctcaacaac gccagcaaat attcccatat tttttgaaca gaagatattg
     1381 atacccgttc gtttggcgaa tgagcgtctt taatcgttgg tccaatcgag atgatatcca
     1441 tagcaggata cgtacctgat ataacaccac actctaaccc agcatgaata acacgaacct
     1501 tgggttcctg accaaacaat gtggtatata catccgtagc atgttttaag aaagcagctt
     1561 caaaacgagg ttcccagccc ggataatggt tttggttaag cacttcagct ccacacaatt
     1621 caaacaattg cctaatacgc actgcaattt catcacgttc ttgctcactc aaactgcgtt
     1681 gatttgtagc gactgtgatg ccattgtgag cagatgatat aaccgccaga ttagtagacg
     1741 tttgcacaac agtaggcatc ttttcactcc aattgataac accgtgcgga cacgctgata
     1801 ctgcttgcaa aaaatcagct agggcatcct tgctccaacc ctctgcaaaa gcatcggttg
     1861 tagagagaac aaaacgcata gaaggctcat aagctaacca ttcttgctcc acatcggcta
     1921 taaaaatatt ccaatcaaca cgtacacttt ctttaaattt gcgaggaacg gcacaccacg
     1981 cataggcttc tcggggaata gcattccgca acgaaccacc ccttacatca cctagccaca
     2041 atgaatactt atctgctata gaaagcaaat aggtatttaa tagttggttt gcattcgccc
     2101 gttttttttc tatatcatcg ccactatgcc cgccttgcaa gccatgtata cttacctcaa
     2161 aaaagaaa
//
LOCUS       DAOPUR010000268         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_54736_length_2156_cov_3.405521, whole genome shotgun sequence.
ACCESSION   DAOPUR010000268 DAOPUR010000000
VERSION     DAOPUR010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54736_length_2156_cov_3.405521"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..660
                     /locus_tag="PKN90_05260"
     CDS             7..660
                     /locus_tag="PKN90_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNZ85720.1"
                     /translation="MDFLGVALFDATSLLHLLMRFVFNMLVCWVIVQFFYYKKSKRRD
                     YYVTFIMFSTTMFLLIFLMKNVDLHIGLTLGIFAIFGMMRYRTETVPIREMTYLFVIT
                     GISVINGLSMTVSYAELVATNLLFVILAYIIENQKVLKHTSSKLVLYEKIDLIVPEKR
                     EELKADLEKRTGLKIEKLEIGHIDFLRDVAFVKIYYVLSPGESNSIDSIGKINQYVG"
     gene            650..1435
                     /locus_tag="PKN90_05265"
     CDS             650..1435
                     /locus_tag="PKN90_05265"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNZ85721.1"
                     /translation="MLGKRNVRFLSILFLGILFVSQVYSQTITYDETEDTDVGLRLSA
                     EISKRLSRNWSISLEEELRLNDNISRFDRLYSSVSVGYTINPYINTSIGYQHRLIQQK
                     GKASTNYQGYLDFRHRGILSVSFSYPVGQFEFSLRQRLNGTLRTDSVNPLEKSNPQII
                     SRTRMQAEYSFWSIPLKPYVSVEMFNPLNNPAYISDVWLETMRYTGGFTYRLDARSRI
                     ELYYFYEDEMGKDVNIGRNSGRVTITTEKEHNHVLGIAYQYRF"
     gene            complement(1432..>2156)
                     /locus_tag="PKN90_05270"
     CDS             complement(1432..>2156)
                     /locus_tag="PKN90_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ85722.1"
                     /translation="LQEEIVQRVQKQERVLITTLTKRMAEELHLYLQRAGVRCTYIHS
                     DVDTLERVEILDHLRSGFYDVLIGVNLLREGLDLPQVSLVAILDADKEGFLRSHRSLT
                     QTAGRAARNLNGKVIMYADKITESMRKTIDETNRRREKQLAYNAEHNITPTAIAQHAH
                     ATLKNKELYTDHPTYSMDNSMVDVAADPIVQYMNKTQLQKSIEKTKKQMQEASKNMEF
                     LEAAQYRNELAKLQEILKSKNS"
BASE COUNT          635 a          316 c          424 g          781 t
ORIGIN      
        1 tctaccattg attttctagg tgtagctcta tttgatgcta ccagtttatt gcatttacta
       61 atgcgttttg ttttcaacat gttagtatgt tgggtgatag tgcagttttt ctattataag
      121 aaaagcaaaa gacgtgatta ttatgttacg tttattatgt ttagcactac catgtttttg
      181 ttgattttct taatgaagaa tgttgattta catattggat taacactagg tatatttgcc
      241 atttttggca tgatgaggta tagaaccgaa acggttccta tccgagagat gacctattta
      301 tttgttatta caggtatttc ggttattaat ggtttgtcga tgactgtcag ctatgctgaa
      361 ttagttgcta caaatttatt atttgttatt cttgcatata ttattgagaa tcaaaaagta
      421 ttaaaacata catcgtcaaa attagtgttg tacgaaaaaa ttgatttaat agttcctgaa
      481 aaaagagagg aactaaaagc cgatttagaa aaacgaacag gattaaagat tgaaaaatta
      541 gaaataggtc atattgattt tttacgtgat gtggcttttg taaaaatata ttatgtatta
      601 agtcctggtg aatcaaatag cattgattca attggaaaaa taaatcaata tgttgggtaa
      661 acgcaatgtt agatttttaa gcatactctt tttaggtata ttatttgttt cgcaggtgta
      721 ttctcagact ataacgtatg acgaaacaga agacactgat gtggggttga gattgtctgc
      781 tgaaatatca aaaagattaa gtcgtaattg gtcaatatcg ttagaggagg agctacggtt
      841 gaatgataac attagtcgat tcgataggtt atattcatct gtatctgtgg gttatacaat
      901 taatccatat attaatacga gtattggcta tcaacatagg ttaatacagc aaaaaggaaa
      961 agcgagtact aattatcaag gatatttaga ttttagacac agaggaatat tgagtgtttc
     1021 attttcctat ccagttggtc aatttgaatt cagtttgcga caacgattaa acggtacctt
     1081 acgtacagat tctgttaatc ccttagaaaa atcaaatcca caaatcattt ctcgtactcg
     1141 aatgcaggct gagtacagtt tttggagtat tcctttaaag ccatatgttt ctgtagaaat
     1201 gtttaatccg ttgaacaatc ctgcttatat tagtgacgtt tggttagaaa caatgcgata
     1261 cacaggaggt tttacttatc gcttggatgc gagaagccga atagaattgt attattttta
     1321 tgaggatgaa atgggtaagg atgtgaatat aggacgcaat agcgggcgag taaccataac
     1381 aaccgaaaaa gagcataacc atgtacttgg aattgcttat caatataggt tttagctatt
     1441 ctttgatttc aaaatttctt gtaattttgc taactcatta cgatactgag cagcctctaa
     1501 gaattccata tttttagagg cttcttgcat ctgttttttt gttttttcta tagatttttg
     1561 gagttgtgtc ttgttcatgt attgaacaat tgggtcagca gcaacatcta ccatgctatt
     1621 gtccattgag tatgttggat ggtctgtgta taattctttg ttttttaacg ttgcgtgtgc
     1681 gtgttgagct attgcagttg gtgtaatatt atgttcggca ttgtacgcaa gttgtttttc
     1741 tcttcgtctg ttcgtttcgt cgattgtttt ccgcatgctt tcggttattt tgtcagcata
     1801 cataatcact tttccgttga ggttgcgtgc ggctctacca gctgtttggg taagcgagcg
     1861 atgtgaacga agaaagcctt ctttatcagc atcaagaata gcgactaacg atacttgtgg
     1921 caaatcaagc ccctctctta gtaagtttac acctattaat acatcataaa atccagaacg
     1981 taagtgatct aatatttcca ccctttcaag cgtatccaca tcggagtgta tgtatgtaca
     2041 tcgtacccct gcacgttgta ggtagagatg caattcttct gccatacgtt ttgtaagagt
     2101 ggtaattagt acacgttctt gtttttgtac acgttgtaca atttcttctt gtaaat
//
LOCUS       DAOPUR010000269         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_54830_length_2154_cov_2.827537, whole genome shotgun sequence.
ACCESSION   DAOPUR010000269 DAOPUR010000000
VERSION     DAOPUR010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54830_length_2154_cov_2.827537"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKN90_05275"
     CDS             complement(<1..583)
                     /locus_tag="PKN90_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNZ85723.1"
                     /translation="MSKVVIIGAGGVGTVVAHKVAQNSKVFTEVLLASRTKSKCDAIA
                     ADVQSRYGVTMQTAQVDADNVPELVALLRSFQPKLVINVALPYQDLTIMDACLEVGVN
                     YLDTANYEPKDEAHFEYSWQWAYQDKFKKAGLTAILGCGFDPGVTSVFTAYAAKHHFD
                     EMHYLDIVDCNAGDHGKAFATNFNPEINIREVSQ"
     gene            complement(603..1730)
                     /gene="dnaN"
                     /locus_tag="PKN90_05280"
     CDS             complement(603..1730)
                     /gene="dnaN"
                     /locus_tag="PKN90_05280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNZ85724.1"
                     /translation="MKFAVSSSHLYERLSSVSRVINTKNTLPILNYFLFSIQGKELSI
                     TASDMETTLITSMELEEVEGEGKVAILAKNLLDILREFADQPLTITIDTASFSVVITS
                     AQGKFSFIGQSGEEYPKMHELDADNTVSFVAPASVLLDGISSTVFATADDELRPVMNG
                     ICFDITTEDLTLVGTDAHKLARFKTTAIKGEKDAMFVLPKKPASLLKSILVKETEPVN
                     VSFDTKNSIFTLSNYKMVCRQIDARYPNYNAVIPKSGPTKIIVDRLALLSGVKRVSVC
                     TNQASSLIRFAISDNQIYLSGQDIDFSISGEEYIPCQFQGEAMEIGFKASFIIELLSN
                     INASDVEFTLIDRTRPGIFTPAEQDDNKNILMLLMPMMLND"
     gene            complement(1805..>2154)
                     /locus_tag="PKN90_05285"
     CDS             complement(1805..>2154)
                     /locus_tag="PKN90_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85725.1"
                     /translation="IIRNEVQVSANDTVALPLGYDRHVNKQYGNRDFILNTVNYLADD
                     EGWMNLRAREFTLRLLNKQAIESQRVFWQIINVVLPLVLLIVCILLYQRSRKMRYGNT
                     RKSSSIKAFFTKK"
BASE COUNT          665 a          425 c          367 g          697 t
ORIGIN      
        1 gttggctcac ttcacggata ttaatttcgg ggttaaaatt ggtagcaaac gctttgccat
       61 ggtcgccagc attgcaatcg acaatatcta aataatgcat ctcatcgaaa tgatgtttgg
      121 ctgcataggc agtaaatacg cttgttacac cagggtcgaa accacaacct aaaatggcag
      181 ttaaacccgc ttttttaaat ttatcttggt aagcccattg ccacgagtac tcaaaatgag
      241 cctcgtcttt gggttcgtag ttggctgtat ctaggtaatt tacacctact tccaaacacg
      301 catccatgat agttaaatct tggtatggaa gagcaacatt aatgactaat tttggctgaa
      361 aagaacgcaa caaagcgact aattcgggca cattatcggc atctacttgt gcagtttgca
      421 tagttacacc ataacgcgat tgcacatcgg cagcaatggc atcacatttt gatttggtgc
      481 ggctagctaa caatacctct gtaaacacct tactattttg agctactttg tgtgctacta
      541 ccgtacctac accgcctgct cctataatta ctacttttga catattactt ttctgttatt
      601 ggttaatcgt ttagcatcat tggcatcaac aacatcaata tatttttatt atcatcttgt
      661 tcggctggtg taaaaatacc aggacgtgta cggtcaataa gcgtaaattc aacatccgaa
      721 gcattgatat tgctcaataa ttcgatgata aaactggctt tgaacccaat ctccatagct
      781 tctccttgga attgacaagg gatatattct tctcctgaaa tagagaaatc aatatcttga
      841 cccgataaat aaatttgatt gtcagagata gcaaaacgga ttaaactact ggcttgatta
      901 gtacatacag atactctttt tacaccagaa agcaacgcca atctatcaac aataatcttg
      961 gtaggtccgc ttttgggtat aacagcattg taattcgggt aacgagcatc tatttggcga
     1021 cacaccattt tgtaatttga taaggtaaaa attgaatttt ttgtatcaaa cgatacgttt
     1081 actggttctg tttcttttac taaaatactt ttgagcaatg aggctggttt tttgggcaat
     1141 acaaacatag catctttttc gcctttgatg gcagttgttt taaaacgtgc caacttatga
     1201 gcatccgtac ctaccaaggt aagatcctca gtggttatat caaaacaaat accattcatt
     1261 acaggacgca attcgtcatc tgccgtagca aacactgtac tcgatatacc atctaacaaa
     1321 acgctagctg gagcaacaaa agaaacggta ttatctgcat ccagttcgtg catcttagga
     1381 tattcttcgc ccgattgtcc aataaaacta aatttacctt gtgcacttgt aatcactacc
     1441 gaaaaactag ctgtatcaat ggtaatagta agtggttgat cggcaaattc gcgtaaaata
     1501 tctagtaagt tttttgccaa gatggctact ttcccttctc cctctacctc ttctaactcc
     1561 attgatgtaa taagggttgt ttccatatcc gaagcggtga tactaagctc tttgccttgt
     1621 atagaaaaca aaaaataatt tagaattggt aaggtatttt tagtgttaat aacgcgacta
     1681 actgaactta gtctttcgta aaggtggcta gatgatactg caaatttcat ttgtatgtag
     1741 ttttaaattt agtttatcga tacttattta tctatattgt ctaaaatcag ttgacaaata
     1801 tacattattt tttagtaaaa aatgctttta tcgatgaaga ttttcgcgta tttccataac
     1861 gcatttttct acttctttga tacaacaaga tacaaacaat gagcaaaact aatggcaaca
     1921 caacattgat aatctgccaa aaaacacgtt gtgattcaat ggcttgtttg tttaacaaac
     1981 gtaatgtaaa ttcccttgca cgcaaattca tccatccttc gtcgtcagct aaataattca
     2041 cggtatttaa aataaagtcg cgatttccgt attgcttatt tacgtgccta tcgtagccta
     2101 aaggcaatgc aactgtatcg ttagcagaca cttgcacttc gttacgaata atat
//
LOCUS       DAOPUR010000270         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_54960_length_2150_cov_3.331265, whole genome shotgun sequence.
ACCESSION   DAOPUR010000270 DAOPUR010000000
VERSION     DAOPUR010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54960_length_2150_cov_3.331265"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1529)
                     /gene="gltA"
                     /locus_tag="PKN90_05290"
     CDS             complement(69..1529)
                     /gene="gltA"
                     /locus_tag="PKN90_05290"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNZ85726.1"
                     /translation="MALVDRSEAWREELRASMKNKERTNIERVHMNELDATIRSRNSE
                     EVNLGLTVEQALLEAKRCLDCANPTCMNGCPVSINIPKFVKNIERGEFLEAAKTLKET
                     SALPAVCGRVCPQELQCESQCIHIKMGKPAVAIGYLERFAADFERESGQISVPEIPHK
                     NGIKVAAVGSGPAGLAFAGDMAQRGYDVTVFEALHQIGGVLKYGIPEFRLPNKIVDVE
                     IECLRQMGVKFLTNCIVGKTITFDELSEEGFKGMFVGSGAGLPNFMNIKGENLIGVMS
                     ANEYLTRVNLMDAANPESDTPVYKGKSVAIIGGGNTAMDAVRTARRLGADRAMIVYRR
                     SEEEMPARIEEIKHAKEEGIEFLTLHNPIEYHGNERGRVTHMLLQKMQLGEPDASGRR
                     SPIAIEGATELIEVDMVIVSVGVSPNPLVPQSIQGLEVSRKGTVIVNENSMQTNIPAI
                     FAGGDIVRGGATVILAMGDGRKAAAAMDEYLKESEN"
     gene            complement(1535..>2150)
                     /locus_tag="PKN90_05295"
     CDS             complement(1535..>2150)
                     /locus_tag="PKN90_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNZ85727.1"
                     /translation="SSTKICNLNGGDYITDLVGPLGKATHIEKFGTVVCAGGGVGLAP
                     LLPIVEALKKAGNRVITVLAARTKDLIILEDQLRKHSDEIVVMTDDGSYGKKGLVTNG
                     VEDIINREKVDLCVTIGPAVMMKFVSLLTKKYEVPTLCSLNTIMVDGTGMCGACRVTV
                     GGKTKFVCVDGPEFDAHQVDFDEMLMRLNAYKDEETESMTNLKK"
BASE COUNT          584 a          491 c          349 g          726 t
ORIGIN      
        1 atgagaaacg accaatgttt ccgtagccta caatggcaac ttttattttg ttcataactt
       61 agtttttttt aattttcaga ttcttttaaa tattcgtcca tagcagcggc ggcttttcta
      121 ccatctccca tggctaaaat aacggttgct ccacctctca caatatctcc acctgcaaaa
      181 atagcaggta tattggtttg catggaattt tcgtttacaa ttacagttcc tttacgagat
      241 acttctaatc cttgaattga ttgaggtaca agtgggtttg gcgaaacacc cacactcact
      301 attaccatat caacttcaat taattcggtg gcaccttcga tggcaatagg cgagcgacga
      361 ccagaagcat caggctctcc caattgcatt ttttgtagca acatgtgtgt tactcttcct
      421 ctttcattgc catgatactc tataggattg tgaagtgtca aaaactcaat gccttcttct
      481 ttggcatgtt taatttcttc gatacgagca ggcatttctt cttccgaacg gcgatataca
      541 atcattgctc tatcagcacc taatctgcgg gcagttctaa cggcatccat agcagtgttt
      601 cctccaccaa taatagcaac acttttgcct ttatacacag gcgtgtctga ttcggggtta
      661 gcggcatcca tcaagttaac acgtgtaagg tattcgtttg ctgacattac gccaattaga
      721 ttttctcctt taatattcat aaagttgggc aacccagcac cgctacctac aaacattcct
      781 ttgaacccct cttcagatag ctcatcgaat gtgatggttt ttcctacgat acaattggtt
      841 aagaatttaa cgcccatttg tctaagacac tcaatttcaa catccacaat tttattgggt
      901 aaacgaaatt caggaatacc atattttaat actccaccaa tttggtgtag tgcttcgaat
      961 acggttacat catatccacg ttgagccata tcgccagcaa aagcaagtcc tgctggtcca
     1021 gagccaacag cagctacttt aatgccattt ttatgaggga tttcaggtac agatatttgt
     1081 ccactttctc tttcaaaatc ggctgcaaaa cgctccaagt aaccaattgc aacagctggt
     1141 ttacccattt ttatgtgaat acattggctt tcgcattgca gctcttgtgg acatacacgt
     1201 ccgcaaacag caggtaatgc cgatgtttct tttagcgttt ttgctgcttc taaaaattcg
     1261 ccccgttcaa tgtttttgac aaattttggt atgttaatgc ttacaggaca cccattcata
     1321 cacgttgggt tggcacagtc aagacagcgt ttagcttcaa gcaatgcttg ttcaacggtt
     1381 aacccaaggt ttacttcttc tgagtttcta cttctaatag tagcatctaa ctcattcata
     1441 tgaacgcgtt caatattcgt acgttctttg tttttcatgc tagcacgtaa ttcttctctc
     1501 cacgcttcgc ttctatctac taatgccatg ctgattattt ttttaaattt gtcattgatt
     1561 ctgtttcttc atctttgtat gcattcaaac gcataagcat ttcgtcaaaa tctacttgat
     1621 gtgcatcaaa ttctggtcca tcaacgcata caaatttggt tttaccacct actgttacgc
     1681 gacaagcccc acacatccct gttccatcaa ccataatagt atttaatgaa cataatgttg
     1741 gtacctcata tttttttgtt aataaagata caaacttcat catcaccgct ggaccaatgg
     1801 taacacataa gtcaactttt tcacggttga taatatcttc tacaccattt gttacaagtc
     1861 cttttttacc atacgaacca tcgtctgtca ttacaactat ttcgtcagaa tgtttacgca
     1921 attgatcttc taaaataatt aagtcttttg tacgtgcagc taaaactgta attactcggt
     1981 ttcctgcttt ttttaatgct tctacgatag gtagaagtgg ggcaagacct acaccaccac
     2041 cagcacatac aactgtgcca aatttttcga tatgagttgc tttgcctaat ggtcctacaa
     2101 ggtctgttat ataatcacct ccgttaagat tacatatttt ggtagatgaa
//
LOCUS       DAOPUR010000271         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_55002_length_2149_cov_2.822350, whole genome shotgun sequence.
ACCESSION   DAOPUR010000271 DAOPUR010000000
VERSION     DAOPUR010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55002_length_2149_cov_2.822350"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PKN90_05300"
     CDS             <1..1122
                     /locus_tag="PKN90_05300"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNZ85728.1"
                     /translation="DIHLQPTTLYIILPVGISFYTFQALSYSIDVYRKQLEPTKDIVS
                     FFAYVSFFPQLVAGPIERATNLLPQFYSKRTFDYDKAVDGMRQILWGLFKKIVIADNC
                     AVYVNQVFDNYEVQTGSTLLIAAIFFAFQIYGDFSGYSDIAIGTARLFGFNLMRNFNF
                     PYFSRDIAEFWRRWHISLSTWFRDYLYIPLGGSRGGKWMSVRNTLIIFTVSGFWHGAN
                     WTFIAWGAFHGLLFLPLLLAKKNRKNTNIVAENRLLPTPIELFQMGTTFLLVVIGWIF
                     FRADSIADAVNYMQGMLQFGTVKAFYRILELKEFLFILILLVVEWLQRNNEHGLESFE
                     NFGKVFKKNKFMRILLYYTILCLILWFSGEEQQFIYFQF"
     gene            1189..2061
                     /locus_tag="PKN90_05305"
     CDS             1189..2061
                     /locus_tag="PKN90_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85729.1"
                     /translation="MEVLLQHIPNDYKYKKEYLEKNAENIETLILGSSHAFFGLNPIY
                     FSKKTFNTSNISQSLNYDYQIVNKYLNNSTSLRTIILPISYFTLFGKLEEGEEAWRVK
                     NYVLYYRLNTATKLTNYSEIFSNKFTVNFDRLYAYYIKNESSISCSTLGWGLNYKSTN
                     AQDLSKTGKIASERHTRKDIHSIESQIIFNENITILQSLIEWCISRNVNIVFITPPAY
                     KTYTQCLNKEQLSITVETMNDICSQYPNCTYLNLLNDTNFVAKDYYDADHLSEIGAKK
                     LSIKMNEILNTQLE"
BASE COUNT          730 a          302 c          361 g          756 t
ORIGIN      
        1 gatatccatt tgcaacctac aactttatac atcattttgc cagttggtat tagtttttat
       61 acctttcaag cattaagcta ttctattgat gtatatcgca aacaattaga gccaacaaaa
      121 gatatagtat cgttttttgc ttatgttagt ttttttcccc agttggtagc cggtcctatc
      181 gaacgagcta ctaatttatt gcctcagttt tatagcaagc gtactttcga ttacgataag
      241 gcagtagatg gtatgcgtca gatattatgg ggtctattta aaaaaatcgt aattgcagat
      301 aattgtgctg tgtatgtaaa tcaagtattt gataattacg aggtgcaaac gggtagtacc
      361 cttttaatag cagctatttt ctttgctttt caaatttatg gcgatttttc tggttattca
      421 gacattgcca ttggaacagc tcgtttgttt ggttttaatt tgatgcgaaa ctttaatttc
      481 ccttattttt caagagatat cgcagaattt tggcgtcgct ggcatatttc tttgtccact
      541 tggtttagag actaccttta tataccgctt ggagggagta gaggaggcaa atggatgtct
      601 gtgcgtaaca ccttgataat ttttacagta agtggttttt ggcatggagc taattggaca
      661 tttattgctt ggggtgcatt ccatgggtta ttgtttttac ccttactatt agcaaagaaa
      721 aatagaaaaa atacaaatat agtggctgaa aatcgcctct tacctacacc tattgaattg
      781 ttccaaatgg gaactacatt tttgttggtt gtaatagggt ggatattttt tagggctgat
      841 agtattgccg atgcagtcaa ctatatgcaa gggatgctac aatttggaac tgtaaaagca
      901 ttttatagaa ttttagaact caaagaattt ttattcatac taatattact tgtagtcgaa
      961 tggttgcaaa gaaacaatga acatggatta gaatcttttg agaattttgg aaaagttttc
     1021 aaaaaaaaca aatttatgcg tattctgttg tattatacta ttctctgttt aattttgtgg
     1081 tttagcggtg aagaacaaca atttatttat tttcaatttt aactaatgaa aagattttta
     1141 ttgaatactc tatatatcct tttacctctt ttattaattg caggtataat ggaagtattg
     1201 ttacaacata ttccaaacga ttacaagtat aagaaggaat atcttgaaaa aaacgcagaa
     1261 aatattgaaa ccctaatatt gggaagttct catgcatttt ttggcttaaa tccgatatat
     1321 ttcagcaaaa aaacctttaa cacgagtaat atctctcaat cattgaatta tgactatcaa
     1381 atagtaaata aatacttgaa taactcaaca tcgctacgta ctattatatt gccaatttct
     1441 tattttactc tttttggaaa actagaggaa ggagaagaag cttggagggt gaaaaattat
     1501 gtgctatatt atagactgaa tactgctaca aaattaacta attattccga gatttttagt
     1561 aataagttta ctgtaaattt tgatagattg tatgcatatt atattaaaaa cgagtctagc
     1621 atctcatgct ctacattagg atgggggcta aattataagt caactaatgc tcaagattta
     1681 agtaaaacag gaaaaattgc tagtgaaaga catactcgta aagatattca ttctattgaa
     1741 agccaaatca tttttaatga aaatataact atattacaat ccttaataga atggtgtata
     1801 agtaggaatg ttaacatagt ttttatcacg ccacctgcgt ataaaacata tacacaatgt
     1861 ttaaataaag agcaattatc gataacagta gaaacaatga atgatatttg ttcacaatat
     1921 ccaaattgta cgtatttgaa cttattgaat gacacaaatt ttgttgcaaa agattattat
     1981 gacgcagatc acctatcaga aataggggca aagaaactat caattaaaat gaatgaaata
     2041 ctaaatactc aattagaata atggagtata taacatacaa gtctttcacc cattagtacg
     2101 ttttagcaaa aatcaattat atgaaagtag cctatttact tggttcgtt
//
LOCUS       DAOPUR010000272         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_55370_length_2140_cov_3.451799, whole genome shotgun sequence.
ACCESSION   DAOPUR010000272 DAOPUR010000000
VERSION     DAOPUR010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55370_length_2140_cov_3.451799"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="PKN90_05310"
     CDS             <1..1836
                     /locus_tag="PKN90_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNZ85730.1"
                     /translation="TEDKEEEICIGSIAELMAEIEKAIQAGMMNKNPFKDFVVNDFSE
                     DNYGISRIDLHRPYVDNIILVSPSGKPMKRETDLIDVWFDSGAMPYAQLHYPFENKAL
                     IDGKTNFPADFISEGVDQTRGWFFTLHAISTMIKGSVAFKNVVSNGLVLDKNGNKMSK
                     RLGNAVDPFSTIETYGSDALRWYMMTNASPWDNLKFDTEGIEEVKRKLFGTLYNTYSF
                     FVLYANVDGFAYKEPEVPMEQRQEIDRWIISLLHSLVKEVDENYADYEPTRAGRAITT
                     FVTDNLSNWYVRLNRRRYWAGEMTLDKLAAYQTLYTCLETVAKLMAPIAPFFAERLFL
                     DLNSVTQKDISESIHLAQFPTFSESLINKQLEERMFIAQTITSLVLALRKKVNLKVRQ
                     PLSSIMIPVLDNNMKEDIEAVKELILNEVNVKSIQFVDNSAGILVKRIKADFKKLGPK
                     CGKHMKQVSTLLQEMSQPQIAEFEKIGNYTLSVENNVNITISTQDVEIISEDIPGWLV
                     ANEGKITIALDITLTDELKEEGIARELVNRIQNIRKANGFDITDRISIVIEKNPLFNH
                     AIENYKQYIASQTLAKSIEISDKINNGIELEFENDKLYLLIQKIA"
     gene            1930..>2140
                     /locus_tag="PKN90_05315"
     CDS             1930..>2140
                     /locus_tag="PKN90_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNZ85731.1"
                     /translation="MGEKTRYSDSELEEFRAIIKDKLEKAFVDYEQLKAALNNLDGND
                     TTDTSPTFKVLEEGATTMSKEEAGRL"
BASE COUNT          803 a          328 c          380 g          629 t
ORIGIN      
        1 acagaggata aagaagaaga aatttgtatt ggttcaattg ccgaattaat ggctgaaatt
       61 gaaaaagcca ttcaagcggg gatgatgaat aaaaatcctt ttaaagattt tgttgtcaat
      121 gatttctctg aagataatta tggaatatct cgtattgacc tacatagacc ttatgtagat
      181 aacatcatat tagtatctcc atcaggaaaa ccaatgaaaa gagaaaccga cctaattgat
      241 gtatggtttg attccggagc aatgccctat gcacaactac attatccttt tgaaaataaa
      301 gcattgatag atggaaagac aaattttcct gctgatttta tttcagaagg tgtagatcaa
      361 acacgtggat ggttctttac tctacatgct atttctacga tgattaaagg ctcggtggca
      421 tttaagaatg tagtttcaaa cggcttagta ttagataaaa atggcaataa aatgtctaag
      481 cgtttgggta atgcagtaga cccatttagc actattgaaa cttatggttc agatgcccta
      541 cgatggtata tgatgaccaa tgcatctcct tgggataact taaaatttga tactgaaggc
      601 attgaagagg taaaacgcaa actatttggc accttataca atacatattc tttttttgtt
      661 ttatacgcta atgtagatgg ttttgcatac aaagaaccag aggttccaat ggaacaacgt
      721 caagaaattg acagatggat aatttctttg cttcattcat tagttaaaga agtagatgaa
      781 aattatgctg attacgaacc aacacgtgct ggaagagcca ttactacatt tgttacagat
      841 aatctaagca attggtatgt gcgattaaac cgcagacgct actgggcagg agaaatgaca
      901 ctagataaac tagctgctta ccaaacattg tatacctgtt tggaaacagt ggcaaaactt
      961 atggctccta tagctccatt ttttgcagaa cgcttatttc ttgatttaaa tagtgtaaca
     1021 caaaaagata tttcagaatc aatacactta gcccaatttc ctactttttc agaatcgttg
     1081 ataaataaac agcttgaaga acgcatgttt attgcccaaa ccatcacgtc cttagtatta
     1141 gctctaagaa aaaaagtaaa tttaaaagta cgtcaacctc tttcctctat tatgattcca
     1201 gtattggaca ataacatgaa agaagacata gaagcagtta aggaattgat tttaaacgaa
     1261 gtaaatgtaa aatcaattca attcgtagac aattcagcgg gaatattggt gaaacggata
     1321 aaagccgatt tcaaaaaatt aggtccaaaa tgtggtaaac acatgaaaca agtatccacc
     1381 ctactacaag aaatgtctca accacaaatt gctgaattcg aaaagatagg aaattatact
     1441 ttgtctgttg aaaacaacgt aaatataact atatccacac aggatgtcga aattatatca
     1501 gaagacatac caggctggtt ggttgcaaac gaaggtaaaa taaccattgc attagatata
     1561 actttgactg acgaattaaa agaagaagga attgcaagag aattggtaaa ccgtatacaa
     1621 aatattcgta aagcaaatgg gtttgacatt acagatagaa tttcgattgt aatcgaaaaa
     1681 aatcctctct ttaaccacgc aattgagaat tataaacaat atatagcttc tcaaacatta
     1741 gctaaaagca tagaaatttc ggataaaata aacaatggaa ttgagttgga gtttgaaaat
     1801 gacaaactat acctattaat tcaaaaaatt gcttgatatt gatatttttt tgaggaatat
     1861 attttgaaat gtgaaaacaa ggagttatat tcgcagtaaa ctttatatta aatactaata
     1921 catacaacta tgggcgaaaa gactagatac tcagatagcg aattggaaga attccgagct
     1981 attataaaag ataaattaga aaaagcattt gttgattacg aacaattgaa agctgcttta
     2041 aacaatttgg atggtaatga taccactgac acctctccta cttttaaagt attagaagaa
     2101 ggtgctacta ctatgtctaa agaagaagct ggcagattag
//
LOCUS       DAOPUR010000273         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_55628_length_2133_cov_2.627045, whole genome shotgun sequence.
ACCESSION   DAOPUR010000273 DAOPUR010000000
VERSION     DAOPUR010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55628_length_2133_cov_2.627045"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKN90_05320"
     CDS             <1..196
                     /locus_tag="PKN90_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85732.1"
                     /translation="VAINKQAIINPTPGQPEQEYLAKYLHEKKFFIAQQQDNIDILKG
                     MEESKSTKIPDMKFYLQLPF"
     gene            complement(197..853)
                     /gene="upp"
                     /locus_tag="PKN90_05325"
     CDS             complement(197..853)
                     /gene="upp"
                     /locus_tag="PKN90_05325"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNZ85733.1"
                     /translation="MRTHNLALQDSLLNTFLEQIRSKEVQKDRMRFRRNMERIGEVMA
                     YEISKSLAYSTKNIATPLGIANVNTVDNDIVLGTIFRAGLPFHQGFLSYFDNADNAFI
                     SAYRKYKDALKFEVFIEYIASPDLTGKTLILVDPMLASGSSMELAYNALLTRGKPKEV
                     HFATIIASQYAVDYLSNHFAEESNISLWSAAIDPELNAHSYIIPGLGDAGDLAFGEKL
                     "
     gene            complement(850..>2133)
                     /locus_tag="PKN90_05330"
     CDS             complement(850..>2133)
                     /locus_tag="PKN90_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ85734.1"
                     /translation="ISFTTKEQVAIDSTVPIICYSREHRIPKSFVIEPEGLLFKRGIK
                     DIQPEVTEWNGTKIFFQSQSADVSIPFDIFAASFYLLTRYEEYGSLDLDEHGRYKAEN
                     SLAYKHQFLDYPLVDHWAYLLDQMLADYFPMYTIKSKSRFRFCPSIDIDHVYKYKSKF
                     LSINIIRLFQKLITGKYEDFRYHLNVILNRAPDPYFNVEYIVDMHNDYNLRPIFFLHV
                     GPMGKYDGYSLYNPFNIIKELFYRTLIVGLHPSYFASTNLHQMKKEKKRLESMTRVRV
                     FLNRFHFLKFTFPISFEYLESLSIARDYSLGYAKYTGFRASTCTPFLFYNLTKDYISG
                     MRLHPIVLMDVTLQKYLYKEATDLFGIMSYYAEVIKKYNGEFVTLFHNESLSDEDQWY
                     GWREEYKKSVQYVALLELHSIEVAKEKLANQHMGV"
BASE COUNT          788 a          389 c          295 g          661 t
ORIGIN      
        1 agtagccata aataaacaag ctattataaa ccctacacct ggacaaccag aacaagaata
       61 tttagcaaaa tatctgcacg agaaaaagtt ctttattgct caacaacaag acaatataga
      121 tattttaaaa ggaatggaag agagtaaaag cacaaaaatt ccagatatga aattctattt
      181 acagttacca ttttaattac aatttttcgc caaaagccaa atcaccagca tctcctaaac
      241 ctggtataat atacgaatga gcattcaatt ctggatcaat agccgcactc cataaagaaa
      301 tattactctc ctcagcaaaa tgattggata aataatcaac agcatactga ctcgcaataa
      361 ttgttgcaaa gtgcacttcc ttaggtttcc ctctggttaa caaggcatta tatgcaagtt
      421 ccatagaaga accactagca agcatggggt ctaccaaaat aagcgtttta ccagtcaaat
      481 caggagaagc aatgtattca ataaaaactt caaacttcaa tgcatcctta tattttctgt
      541 atgcagatat aaaagcatta tccgcattat cgaaatacga caaaaaacct tgatgaaatg
      601 gtaaacctgc tctaaatata gtacctagca caatatcatt atcaacggta tttacgttag
      661 ctatacccag tggagtagca atatttttag ttgaatatgc taatgattta ctaatctcat
      721 aagccattac ctcgccaata cgctccatat tccgtctaaa acgcatccta tctttttgaa
      781 cctcttttga acgtatttgt tctaaaaaag tatttagtaa agagtcttgc aaagctaaat
      841 tgtgtgttct catactccca tatgttgatt tgctaatttt tcttttgcta cttcaataga
      901 atgcaactca agaagtgcaa catattgcac tgatttttta tattcttccc tccatccata
      961 ccattggtct tcgtctgata atgattcgtt atgaaacaat gtaacgaact ctccgttata
     1021 tttcttaatc acttcagcat aataactcat aatcccaaac aaatctgtag cttctttata
     1081 taaatatttt tgcaaagtaa catccattaa aacaatagga tgcagacgca tcccagaaat
     1141 ataatctttg gttaaattat aaaacaaaaa tggagtgcaa gtactcgctc taaagcctgt
     1201 atattttgcg tatcctaagg aataatcacg ggcaattgat aatgattcga gatattcaaa
     1261 gctaatagga aaagtaaatt tcaaaaaatg gaatctattt aaaaaaacac gaacccttgt
     1321 catcgactct aagcgttttt tttctttttt catttgatgt agatttgtac tagcaaaata
     1381 tgatggatgc aaaccaacaa tcaacgtacg ataaaacaat tccttaatga tattaaatgg
     1441 attgtataat gaatatccat catatttacc cattggacct acgtgaagaa agaaaatagg
     1501 acgcagatta taatcattgt gcatatcaac tatatactca acattaaaat aaggatcagg
     1561 ggctctattc aaaataacat ttaaatgata tctaaaatcc tcatatttcc cagttatgag
     1621 tttttgaaat aaacgaataa tattaattga aagaaacttc gacttatatt tatacacatg
     1681 atcaatatca atagaaggac aaaatcgaaa acgagattta gattttatag tatacatggg
     1741 aaaataatcc gccaacatct gatctaacaa atatgcccaa tgatctacca agggataatc
     1801 taaaaattga tgtttatatg ccaaactatt ttctgcttta tatcttccat gctcatctaa
     1861 gtcaagacta ccatattcct cgtatcgcgt cagaagataa aaagatgcgg caaaaatatc
     1921 aaatggtata gaaacatcgg ctgattgtga ttgaaaaaat atttttgttc cattccattc
     1981 tgtaacttct ggttgaatat ctttaattcc ccttttaaac aataaccctt caggctcaat
     2041 gacaaaggat tttggaatac gatgttctct agagtaacaa ataataggaa cagtactatc
     2101 aatagcaact tgctcctttg tagtaaaaga tat
//
LOCUS       DAOPUR010000274         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_55650_length_2132_cov_3.690419, whole genome shotgun sequence.
ACCESSION   DAOPUR010000274 DAOPUR010000000
VERSION     DAOPUR010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55650_length_2132_cov_3.690419"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PKN90_05335"
     CDS             complement(<1..656)
                     /locus_tag="PKN90_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNZ85735.1"
                     /translation="MIKILDTTLRDGEQTSGVSFAIQEKLSIARLLLEDLQIDRIEVA
                     SARVSDGEFASVQQITKWAKSKNVLDKVEILGFVDGFTSLKWIEKAGGRVINLLCKGS
                     LNHCTHQLRKTPQEHIADIKQVVANAQSMGITVNIYLEDWSNGIQQSSEYVFTLMDSL
                     KDEPIVHFMLPDTLGILNPNQTRTYCSMMVERYPSLQFDFHAHNDYDMAVANVFEAVQ
                     V"
     gene            complement(669..1280)
                     /gene="leuD"
                     /locus_tag="PKN90_05340"
     CDS             complement(669..1280)
                     /gene="leuD"
                     /locus_tag="PKN90_05340"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNZ85736.1"
                     /translation="MEKFKTISSTVVPLPIENVDTDQIIPARFLKATSKLGFGDNLFR
                     DWRYDKNDQPNPDFVLNNPTYSGEILVAGKNFGSGSSREHAAWAIAGYGFKIVISSFF
                     ADIFKNNAMNNGVLPVVVSEAFLAEIFSSVENNPKTIITVDLQNQTVTNTATNKSEKF
                     DINSYKKECFLQGFDDIDYLLAKKQIIEQYEDKQSDVAYFLTT"
     gene            complement(1328..>2132)
                     /locus_tag="PKN90_05345"
     CDS             complement(1328..>2132)
                     /locus_tag="PKN90_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNZ85737.1"
                     /translation="GYFVEYAGSAVQALSMEGRLTLCNLSIEMGARGGMVAPDETTFE
                     YIKGREFAPKGEEWDKALAYWKSLPTDEGAQFDKDIAFDAADIEPMITYGTNPGMGLG
                     ITESIPSLDSIDEAGRISFVKSLDYMGFKPGDKMIGKPIDYVFLGSCTNGRIEDFRAF
                     ASLVKGKKKADNVVAWLVPGSWAVEKQIKEEGIDTILTEAGFVLRQPGCSACLAMNDD
                     KVPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAAAAAITGKITDPRTL"
BASE COUNT          649 a          459 c          321 g          703 t
ORIGIN      
        1 acttgtactg cctcaaatac atttgcaaca gccatatcgt aatcgttatg agcatgaaaa
       61 tcaaactgca acgaaggata tcgttcaacc atcattgaac aatacgtgcg agtttggttt
      121 ggatttagaa tccccagtgt atctggtaac atgaaatgta caattggttc atcttttaga
      181 ctgtccatta aggtaaaaac atattcagaa ctttgctgta tgccattcga ccaatcttct
      241 aggtatatat ttactgtaat acccattgat tgtgcgttgg caactacttg cttaatgtca
      301 gctatgtgct cttggggtgt ttttcttagc tgatgagtac aatgatttaa cgagccttta
      361 caaagtaaat taattactct ccctcccgct ttttctatcc atttaaggga tgtaaaacca
      421 tcgacaaaac caagaatttc aaccttatcc aatacatttt tgctttttgc ccatttggta
      481 atttgttgaa cagaagcaaa ttcgccatcc gacacgcgtg cagaagccac ttcaattcta
      541 tcaatttgta aatcttctag caataagcgt gcgatactca gcttttcttg tatagcaaac
      601 gaaacccctg aggtttgctc tccatcacgc aatgttgtat ctaaaatttt tatcattctt
      661 tgtgtcaatt aagttgtaag aaaataagct acatcagatt gcttatcttc gtattgttca
      721 attatttgct ttttagctaa taaataatca atatcatcaa atccttgcaa aaaacactct
      781 tttttatacg aatttatatc aaatttttcg gatttatttg tagcagtgtt agttactgtt
      841 tggttttgaa gatctactgt aataatcgtc ttagggttat tttcaacaga agagaatatt
      901 tcggctaaaa atgcttcaga aaccactact ggcaataccc cattgttcat ggcattattt
      961 ttaaaaatat ccgcaaaaaa acttgatatt acaatcttaa atccgtaccc tgcaatagcc
     1021 catgcagcat gttcgcgact tgaaccacta ccaaaatttt tacctgctac caatatttcg
     1081 ccactatagg ttggattatt aagcacaaaa tcaggatttg gttggtcatt tttatcgtat
     1141 ctccaatcac gaaaaagatt atctccaaag cctaatttgg aagtagcttt taaaaaccgt
     1201 gctggtataa tttgatcagt atccacattt tcgataggca aaggcactac tgtactactg
     1261 attgttttaa acttttccat attctatcta tattactata ctattaatac taaaaatcat
     1321 ttactctcta caacgttctt ggatcggtaa ttttacccgt tatcgcagct gctgcagcaa
     1381 ctaacggacc tgccaaaatt gtgcgtgctc caggtccttg acgaccttca aaatttctgt
     1441 ttgaagtcga aacagcatat ttaccagcag gaactttatc atcgttcatc gccaaacatg
     1501 ccgaacaacc aggttgacga agcacaaaac ctgcttcggt taaaatggta tcaataccct
     1561 cttcttttat ttgcttttca acagcccacg aaccaggaac taaccatgct acaacattat
     1621 cagctttttt ctttccttta actagcgaag caaatgctct aaaatcttca atacgtccat
     1681 tcgtacagct tccaagaaat acataatcaa tgggtttacc tatcatttta tctccaggtt
     1741 taaagcccat ataatccaat gatttaacaa aagaaatgcg acctgcctca tcaatactat
     1801 ccaatgaagg aattgattcg gtaattccta accccattcc tggatttgtt ccataggtaa
     1861 tcattggttc aatatcagct gcatcgaatg caatatcttt atcaaattga gccccttcgt
     1921 cagttggtaa tgatttccaa tacgctaagg ctttatccca ttcttcgcct tttggagcaa
     1981 attcacgacc tttgatgtat tcgaaagtag tttcatctgg agcaaccata cctccacgag
     2041 ctcccatttc gattgaaaga ttacacaagg ttaaacgacc ttccatcgaa agtgcttgta
     2101 cggcactacc tgcatattct acaaaatacc ca
//
LOCUS       DAOPUR010000275         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_55947_length_2124_cov_3.040599, whole genome shotgun sequence.
ACCESSION   DAOPUR010000275 DAOPUR010000000
VERSION     DAOPUR010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55947_length_2124_cov_3.040599"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKN90_05350"
     CDS             complement(<1..500)
                     /locus_tag="PKN90_05350"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNZ85738.1"
                     /translation="MRKNIVAGNWKMNKTLQEGIALATELKDALVGTTPNCDVVIGTP
                     FIHLATVVETVKKSPIKVAAQNCADKESGAYTGEVSASMIKSTGVNYVILGHSERRAY
                     YGETFEILRNKVLLALKNELTPIFCIGELKEEREANKQNEVVKAQLEGSVFNLSATDF
                     GKIVIA"
     gene            complement(513..1751)
                     /locus_tag="PKN90_05355"
     CDS             complement(513..1751)
                     /locus_tag="PKN90_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNZ85739.1"
                     /translation="MKQLVLFICRILFGLVFMFSGFVKAVDPLGSMYKFEDYFIAFGF
                     TSLAPLALFLAIALAALEFLIGANLVLGVHIKKTSWLALFFMAFMTPFTLYIAIANPV
                     ADCGCFGDAWIIDNWTTFYKNILFSILIITIFIFRNEEKIHLANRTQWMMSGFAILFS
                     VAVSVYGYMRLPIFDFRPYKIGANIIEGMTIPENAPQDEYLTTFIYEKEGKQKEFTLD
                     NFPTGDSTWTFVKQQSVLISKGFRPAIHDFSIDYQGEDITDIVLEDSNYTFLVISSKL
                     EDYSQCYSDKINKLYAYAQQNGYSFYCLTSSFGDAVENFRTLTGANYPIAFSDEVTLK
                     TIIRTNPGVLLVKNATVYNKWHCANLPDFSAPIEDTVFIDVNYPHLWKTVVWLLLLFS
                     IPVALIYVLDKKSTIHNKTN"
     gene            complement(1754..>2124)
                     /locus_tag="PKN90_05360"
     CDS             complement(1754..>2124)
                     /locus_tag="PKN90_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide modification associated
                     domain-containing protein"
                     /protein_id="HNZ85740.1"
                     /translation="KIEEDIRAEFIAIVNYGIIGLIQLELGVAEVDDLPAEKALVIYD
                     GYAQKAFDLMMDKNHDYDEAWRSMRISSYTDLILMKIYRTKQIEDNDGKTLISEGVDA
                     NYFDMINYAIFGLIKLHYES"
BASE COUNT          717 a          426 c          277 g          704 t
ORIGIN      
        1 taagcaataa ctattttacc aaaatctgta gcagacaaat taaaaactga accttctaat
       61 tgggctttta ctacttcatt ttgtttattt gcttcacgtt cttcctttaa ttcgccaata
      121 caaaatatag gtgtaagttc attttttaat gcaagcaata ctttatttct taaaatttca
      181 aaagtttcgc cataataagc tcttctttct gaatgtccca aaatcacata attaacgcct
      241 gtagatttaa tcatagaagc tgaaacctct ccagtataag cccctgattc tttatcagca
      301 caattttgag ccgctacttt tataggtgat ttttttacag tttctactac tgttgctaaa
      361 tgaataaagg gtgtgccaat tacaacatca caattaggtg ttgttcctac caaagcatcc
      421 tttaactccg ttgctaatgc aataccctct tgtagggttt tgttcatttt ccagtttcct
      481 gctacaatgt tttttctcat tttcgttttt gtttaatttg ttttattatg aatcgttgat
      541 tttttatcta acacatatat tagagctaca ggtatactaa ataataaaag taaccacact
      601 accgtcttcc ataagtgagg ataattcaca tcaataaata cagtatcctc tatcggggct
      661 gaaaaatcgg gaagatttgc acaatgccac ttattataca cggttgcatt tttcaccaac
      721 aatacacctg gatttgttct tataatggtt ttcagtgtta cctcatcaga aaatgctatt
      781 ggataatttg cccctgttag cgttctaaaa ttttctactg catcgccaaa agatgacgtt
      841 aggcaataaa acgaatatcc attttgttgt gcataagcat acaacttgtt tatcttatca
      901 gaataacact gactataatc ttctaactta gagcttatca ctaaaaaagt ataattacta
      961 tcttccaata caatatctgt aatatcctct ccttgataat caattgaaaa atcatgtatt
     1021 gcaggacgaa aaccttttga tataagtacg gattgttgtt tcacaaatgt ccaagtagaa
     1081 tctcctgttg gaaaattatc taaagtgaac tccttttgct ttccttcttt ttcgtatata
     1141 aaggtagtga gatattcatc ctgtggggca ttctctggaa tggtcattcc ttcaattata
     1201 tttgctccaa ttttatacgg acgaaaatca aatattggca accgcatata cccatacaca
     1261 gaaacagcaa cactaaacaa tatagcaaaa ccactcatca tccattgtgt tctgtttgcc
     1321 agatgaatct tttcctcatt acgaaaaata aaaatagtga taatcaaaat agaaaataag
     1381 atatttttgt aaaaggtcgt ccaattatca ataatccaag catcgccaaa acatccacaa
     1441 tcggctactg gattagcaat agcaatgtat aaagtaaagg gagtcataaa agccatgaaa
     1501 aacaacgcca accatgatgt ttttttaata tgcaccccta atactaggtt agctccaata
     1561 agaaactcta atgcagccaa cgcaatagcc aaaaacaacg ccaacggagc cagcgaagta
     1621 aaaccaaagg ctataaaata atcttcaaat ttgtacatcg aaccaagtgg atccacagcc
     1681 ttaacaaagc ctgaaaacat aaacaccagt ccaaataaaa ttctacaaat aaataacact
     1741 aattgtttca tctctaagat tcgtaatgca atttaattaa tccaaatata gcataattaa
     1801 tcatatcaaa atagttggca tccacccctt ctgatattaa cgttttccca tcgttatctt
     1861 caatttgttt tgttctgtat atcttcatca gtattaaatc tgtatacgaa ctaatacgca
     1921 tactacgcca agcttcgtca taatcatgat ttttatccat catcaaatcg aaagcttttt
     1981 gagcatagcc atcgtagatt accaaggctt tttctgctgg taaatcatct acttctgcaa
     2041 cacctaactc taactgtatc aaaccaataa taccataatt tactatagct ataaattcag
     2101 cacgaatatc ttcctcaatt tttg
//
LOCUS       DAOPUR010000276         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_56071_length_2121_cov_3.711520, whole genome shotgun sequence.
ACCESSION   DAOPUR010000276 DAOPUR010000000
VERSION     DAOPUR010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56071_length_2121_cov_3.711520"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1654)
                     /locus_tag="PKN90_05365"
     CDS             complement(98..1654)
                     /locus_tag="PKN90_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ85741.1"
                     /translation="MKINLLQKVILFLTLIAFFACKNDKNVSNTTGWKYNDKKHSGFQ
                     VVHDYEQDTPPGMVLIEGGTFTMGRVTEDTYSEWNNYPRRLTVSTFYMDQHEVSNLDW
                     REYVYWMRLMFSASPKLVNRALPDTLVWRDELAYNEPYLEYYFSHVAYQEYPVVGVSW
                     EQAVDYCLWRTDRVNELRMVQAGVIELPDFKALHKDSATVEKQDSFARKQLFNVDKYL
                     KNSDYKPDKEKSKVKTVFGDARKTNMSDGILLPQYRLPTEAEWEFAAYGIKSEEGMEN
                     YDERRIFPWDGSQLRNPTKKHRGKMMANFVRGRGDYMGVAGDLNDKASITAPVSSFWP
                     NDYGLFNMAGNVNEWVMDVYRPMTSENVQEYNPFRGNMYVSPIFNDSISDAGTTRIVK
                     LDSLGRAVKAIAVSKDSISDYVKYDVRNYEDGDAKTSVDANQWKNDIDPDESTKRLYN
                     PDTDAEGMLATHISNTTRVFKGGGWKDRAYWLNPATRRYLEQTKSRSDLGFRCAMSRV
                     GSDKGNQDKK"
     gene            1847..>2121
                     /locus_tag="PKN90_05370"
     CDS             1847..>2121
                     /locus_tag="PKN90_05370"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNZ85742.1"
                     /translation="MIHTNAHLSIRVKGKLLQLHTPLVMAIINVTPDSFYTKSRINLP
                     NEISTAVCSSLHKGATIIDIGAYSSRPNATDISEKEEINRLDVALSA"
BASE COUNT          623 a          439 c          322 g          737 t
ORIGIN      
        1 gcgtttgtat attcatatag atagtataat ttacgtcaaa aaaggatagc ttatctagct
       61 atccttttct atattttttc aataaacttt ctacagctta ttttttatct tgatttcctt
      121 tgtcagaacc tacacggctc atagcacaac ggaaacctaa atcgctacga ctttttgttt
      181 gttccaaata acgccttgtt gcaggattta accaatacgc tctatctttc caaccaccac
      241 ctttaaatac acgagttgta ttcgagatat gagtagctaa cattccttct gcatcggtat
      301 caggattata taaacgtttg gttgattcgt ctgggtcaat atcatttttc cattggtttg
      361 catcaacact tgtttttgca tcaccatctt catagttacg tacatcatat ttcacgtaat
      421 cagaaattga atccttactt actgctatgg cttttacagc tcttcctaag ctatcaagtt
      481 ttacaattct tgttgttcct gcatcagaaa ttgaatcatt aaaaataggc gaaacataca
      541 tgttaccacg gaaaggatta tattcttgaa cgttctcgct agtcattgga cgatatacat
      601 ccattaccca ctcgtttacg ttacccgcca tattgaacaa accataatcg ttgggccaaa
      661 atgaactcac tggagcagta atcgatgctt tatcattcaa atcacctgca acacccatgt
      721 agtcaccacg accccttaca aagtttgcca tcattttccc tctatgtttt tttgttgggt
      781 ttcttagctg agagccatcc catgggaata tacgtctttc gtcatagttt tccattcctt
      841 cttctgattt gataccataa gcagcaaact cccattctgc ctcagttggc aggcggtatt
      901 gtggcaacaa aattccatca gacatatttg ttttacgagc atctccaaac actgttttta
      961 ctttgctctt ttctttgtct ggtttataat cagaattttt taggtattta tccacattaa
     1021 atagttgttt tctagcaaaa ctatcttgct tttcaacagt tgctgaatcc ttgtgcaagg
     1081 ctttaaagtc gggtaattca ataacgccag cttgtaccat acgtaactca tttacacggt
     1141 ctgttctcca taagcagtaa tctaccgctt gctcccagct aacaccaacc acaggatatt
     1201 cttggtaggc tacatgcgaa aaataatact ctaaataagg ttcattataa gctaactcgt
     1261 ccctccatac caatgtatca ggcaatgctc tgtttactaa ttttggtgat gcagaaaaca
     1321 ttaacctcat ccaatacaca tattctctcc aatctagatt ggaaacttcg tgctgatcca
     1381 tataaaaagt actaactgtt aaacgacgag gataattatt ccactcactg tatgtatctt
     1441 cggtaacacg acccatagta aaggtacccc cttctattaa aaccatgcct ggaggagtgt
     1501 cttgctcata atcgtgaaca acttgaaatc cactgtgttt tttgtcgttg tatttccaac
     1561 cagttgtatt ggaaacgttt ttatcgtttt tacaggcgaa aaaagcgata agagttaaaa
     1621 aaagaataac tttttgaaga agattgattt tcatagtttg actcaaaatt taaaattggc
     1681 ttacaaagat aacagatttt tgtgtttctt attgtgtgat ttaggtattt tttttaaata
     1741 tctgtaaaaa actgtgcaaa gatgaagata aatttttaat tttgcaaagc attcgggtgg
     1801 ttttccctat tttttcatac attttatttc tatcattacc atttttatga tacatacaaa
     1861 cgcacattta agtattcgtg tcaaagggaa attactacaa ttacatactc ctttggtaat
     1921 ggctattatt aatgttactc ccgactcttt ctatacaaaa agccgtataa atctccctaa
     1981 tgagatatct actgcggtgt gcagctcttt acataaagga gctacaatta ttgatatcgg
     2041 agcctattca tctcgaccaa atgccactga catttcagaa aaagaagaaa tcaatcgttt
     2101 ggacgtagcc ctttctgcct g
//
LOCUS       DAOPUR010000277         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_56115_length_2120_cov_4.155448, whole genome shotgun sequence.
ACCESSION   DAOPUR010000277 DAOPUR010000000
VERSION     DAOPUR010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56115_length_2120_cov_4.155448"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PKN90_05375"
     CDS             <1..1161
                     /locus_tag="PKN90_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HNZ85743.1"
                     /translation="GEVLTFDGFLKVYMESNDDEDTTEDTRMLPPLHVHDTLQYKKIL
                     STERFTQRPPRYTEASLVRKLEELGIGRPSTYAPTISTIQNREYVEKGDKEGEKRTYN
                     LLTLEKGTITDIIKEEIVGTEKSKLFPTDIGSVVTDFLLANFASVVDYNFTAKVEKEF
                     DIVASGKLEWQKAIDKFYKGFHTLVEDSLQNSTKKVGERLLGNDPTTGKPISVKIGRY
                     GAIAQIGLTTEEEKPQFAALKKEQSIETITLEEALELFKLPRTVGEFEEKEVTIAVGR
                     FGPYVKHASAFYSIPKEIDPLKITLSGAIDLIEKKREDDKNKVLKTFAEDASIQLLNG
                     RYGAYIKQNKNNYKIPKSKKAEELTYEDCLEIIKSEASDGDKPKKKKGFKKK"
     gene            complement(1169..1684)
                     /locus_tag="PKN90_05380"
     CDS             complement(1169..1684)
                     /locus_tag="PKN90_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85744.1"
                     /translation="MNKEVLFSESQRFKQWWLWGIVIAINALLLYGVYVQIMLGQAFG
                     NNPMTNTQLIIVTLLTIMLAFLFLSFRLDTIIRKDGIYVRFFPFHIRFKYYDWQILSK
                     FYVRPYYPITEYGGWGLRLGLFGKGVAYTVSGNMGLQLEFTHNKKLLIGTNKPTELAK
                     VLSQLEQFKQE"
     gene            complement(1742..>2120)
                     /locus_tag="PKN90_05385"
     CDS             complement(1742..>2120)
                     /locus_tag="PKN90_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNZ85745.1"
                     /translation="AVAKHGNERMSQQILAQTGLSVAGVALSGKSEQAQQLMSSVYGL
                     GVNYGVILPYSRKHEYEADRLGMIFMAMAGYDPAGAVAFWERMTAGKESSVPEFLSTH
                     PSDTKRIQAMQNVLPEAQGYYKK"
BASE COUNT          768 a          391 c          379 g          582 t
ORIGIN      
        1 ggcgaggtgc taacttttga tggtttttta aaagtatata tggagtcaaa tgacgatgaa
       61 gatacaacag aagatacaag aatgctacct ccactgcatg tacatgacac tttgcagtac
      121 aaaaaaatac ttagtaccga acgatttaca caacgaccac cacgatacac ggaggctagt
      181 ttggtacgta aattagaaga gttgggcatt ggacgaccat ctacctacgc acctactatc
      241 agtacaattc aaaatagaga atacgtagaa aaaggagata aagaaggtga aaaaagaacg
      301 tataatttgc ttacattaga aaaaggtaca attacagata tcataaaaga ggaaattgtt
      361 ggtacagaaa aatcaaaact ttttccaacc gatataggtt cggtcgtaac agatttttta
      421 ctagccaact ttgcttcggt tgtagactat aattttacag ctaaagtaga aaaagaattt
      481 gatatagttg cctcaggaaa attggaatgg caaaaagcaa ttgacaaatt ttacaaaggg
      541 tttcatactc ttgttgaaga ttcgttgcaa aattccacta aaaaagtagg agaaagactt
      601 ttgggaaacg acccaacaac aggaaaaccg atatctgtaa aaattggacg ctatggggct
      661 atagcccaaa ttgggttaac taccgaagaa gaaaagccac aatttgctgc actaaaaaaa
      721 gaacaatcta tcgagacgat tacactcgaa gaggcattag aattatttaa actaccacga
      781 actgtgggtg agtttgagga aaaagaagta acaatagccg ttgggcgttt tggtccttac
      841 gtaaaacacg cctctgcatt ttattctatc cccaaagaaa ttgacccttt aaaaataaca
      901 ctgtcgggag caatcgattt gatagaaaag aaacgcgaag acgacaaaaa taaagtacta
      961 aaaacatttg cagaagatgc ctctatacaa ctattaaatg gacgttatgg tgcgtacata
     1021 aaacaaaata aaaacaatta caaaatacca aagtcgaaaa aagcagaaga gctcacatac
     1081 gaagattgct tagagattat taaatcagaa gcaagcgatg gagataaacc taaaaagaaa
     1141 aaaggattta aaaagaagta agaaagaatt actcttgctt gaattgttct aattgactta
     1201 gtacttttgc taattctgtt ggtttgtttg ttccaataag gagtttttta ttatgtgtaa
     1261 actcaagttg taaacccata ttaccagata cagtataagc aacgcccttt ccaaagagtc
     1321 caagacgtaa accccaaccg ccatattcag taattggata gtagggtctt acataaaact
     1381 tgctcagtat ttgccaatca tagtatttga atcgtatatg aaaaggaaaa aaccttacat
     1441 aaataccatc ttttcgaata attgtatcga gacgaaagct caaaaataaa aaagcaagca
     1501 taattgtcaa aagggttact atgataagtt gagtgttcgt cattggatta ttcccgaaag
     1561 cctgcccaag cataatttgc acatatacac catacaacaa aagtgcatta atcgctataa
     1621 caatacccca taaccaccat tgtttaaatc gctgactttc agaaaataag acttctttgt
     1681 tcatggaata tcgttttttg gaatattaag cctttcaacg tttctagtaa accgtaataa
     1741 ctcatttttt ataataccct tgtgcctctg gtaatacatt ttgcatagct tgtattcgtt
     1801 ttgtatcaga ggggtgagtg ctcaaaaatt ctggcacact gctttcttta cctgctgtca
     1861 tacgttccca aaaagctacg gcaccagctg ggtcgtaacc tgccattgcc ataaaaatca
     1921 ttccaagcct atctgcttca tactcatgct tacgcgaata aggcaaaata acaccgtaat
     1981 ttactcccaa gccatacact gaactcataa gctgttgtgc ttgttctgat ttacctgaca
     2041 aagccactcc tgctacgctc aatccggttt gagcaagtat ttgctggctc atacgttcgt
     2101 tgccatgttt tgcaacggca
//
LOCUS       DAOPUR010000278         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_56449_length_2111_cov_2.874027, whole genome shotgun sequence.
ACCESSION   DAOPUR010000278 DAOPUR010000000
VERSION     DAOPUR010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56449_length_2111_cov_2.874027"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..332)
                     /locus_tag="PKN90_05390"
     CDS             complement(201..332)
                     /locus_tag="PKN90_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85746.1"
                     /translation="MTLGALMSVSYIIGQMNSPVNQLVNFFRSLQDARLSVERLNEV"
     gene            complement(400..795)
                     /locus_tag="PKN90_05395"
     CDS             complement(400..795)
                     /locus_tag="PKN90_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HNZ85747.1"
                     /translation="MKFVPQHDMMDCGPACLSMIANHYGKDYSVQYLRETTYITREGV
                     SLLGITQAATDIGFSTLPTKLTLPKLKELSLAKQVPFPCVLHWNQTHFVLLCKVSKNP
                     FTGKFQFKLADPEHGFITLSEDKFIKGWF"
     gene            complement(812..1432)
                     /locus_tag="PKN90_05400"
     CDS             complement(812..1432)
                     /locus_tag="PKN90_05400"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNZ85748.1"
                     /translation="MKNLLLISILFINAIIMQSQVVTETRIETSDDILVIQIDGLPNI
                     CIRNGNIYKVEVEAEKKNQSHIKTSVKDKKIPIISRKFFESSHYIVYITIPNKSIHIK
                     ANDVANIYIDKNTTLQIDTISLSANAVANIYLDKIITNTLNCKLNSVANITIKGSANK
                     CNLNFKSVANTKLNEFKIKDTNLLLKDVANFELNTTENNDDFDIKK"
     gene            complement(1457..1873)
                     /locus_tag="PKN90_05405"
     CDS             complement(1457..1873)
                     /locus_tag="PKN90_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNZ85749.1"
                     /translation="MNNDILDIISVFKANNLKTSYNKYNDSVRESCYGDKKNQATINF
                     NGDVYKCTARDFKPENREGVLMDDGTIEWNDKYYQRMESKFNNPPCLACNILPICNGS
                     CTQHALDNIGKDYCVHDFDENKKIDIIREKLLYAIN"
     gene            complement(1919..>2111)
                     /locus_tag="PKN90_05410"
     CDS             complement(1919..>2111)
                     /locus_tag="PKN90_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNZ85750.1"
                     /translation="QDRYWVYSEAAETNPAGPSSGTTRVFRGGVCDYEASDCTVTARR
                     SDRPANKYYNLGFRLVMVP"
BASE COUNT          630 a          353 c          329 g          799 t
ORIGIN      
        1 ctgccactgg tgcctacaat ggcagttatt ttcccttcgg gtatgtttag gtcaatgcct
       61 tttagcacat aaggcgacat cggtccttgg tactgaaacg aaatattttt caaatgaata
      121 cctttagtca tttgttcatg tacattggta ttatcaggcg aataatatat ttgatcgggt
      181 tgttcttctt caagatgata ttacacctcg ttgagtcgtt ctacagataa acgggcatct
      241 tgtagcgacc gaaaaaaatt aactaattga tttacaggag aattcatctg tccaataata
      301 tacgaaacac tcattaatgc tccaagagtc atttgacctt gtactacgta gtaggcagct
      361 aaaaaagtaa ctaatatgtt tttaagttga ttaaaaaact caaaaccagc cttttataaa
      421 tttatcttcg gataaagtaa taaaaccatg ttcaggatcg gcaagtttaa attgaaactt
      481 ccctgtaaaa gggttttttg aaactttaca caataaaacg aaatgcgttt gattccaatg
      541 caaaacacat ggaaaaggca cttgttttgc aagtgataac tcttttagtt ttggtaatgt
      601 tagttttgtt ggtaatgtag aaaaacctat gtcggtagct gcttgtgtaa tacccaaaag
      661 agatacccct tctcgtgtaa tataagtagt ttcacgtaag tattgtacac tataatcttt
      721 accataatgg ttagctatca tcgaaagaca agctggacca caatccatca tatcgtgctg
      781 aggaacaaat ttcatcaatc tattttaaaa attatttctt aatatcaaaa tcatcgttat
      841 tttcggttgt gtttagttca aaatttgcta catctttgag taataaattg gtatctttaa
      901 ttttaaattc attcaatttt gtatttgcta ccgatttgaa atttaaatta cacttgttag
      961 ccgacccttt tattgtgata tttgctactg agtttaattt acaattcaac gtatttgtaa
     1021 ttattttgtc taaataaata tttgccacag catttgcact caaggaaatt gtatctatct
     1081 gcaatgttgt atttttatca atataaatat ttgctacatc gtttgctttt atgtgtatcg
     1141 atttatttgg aatagtgata tatacgatat aatgacttga ttcaaagaat tttctactta
     1201 taattggtat ttttttatct tttacagatg tttttatatg tgattgattc tttttttcag
     1261 cttccacttc tactttgtat atgttgccat ttcttataca aatatttggt aatccatcta
     1321 tttgtataac caaaatatca tcagaagttt cgatacgtgt ttctgttact acttgtgatt
     1381 gcataatgat tgcattgata aataaaatac tgattaataa taagtttttc ataaaatgta
     1441 ggtttttaat ttgtttttag ttaatggcgt ataataattt ttcgcgtata atatctattt
     1501 ttttattctc gtcaaaatcg tgtacgcaat aatctttacc tatattatct aatgcatgct
     1561 gagtacatga accattacaa ataggtagga tattacaagc aaggcagggt ggattattga
     1621 attttgactc catgcgttgg tagtatttat cgttccactc tatcgtccca tcgtccatta
     1681 aaacaccttc acgattctct ggtttaaaat ctcttgccgt gcatttatat acatcaccat
     1741 tgaagttaat agtggcttgg ttttttttgt ccccataaca cgattctctg acagagtcat
     1801 tgtatttatt gtaacttgtt ttgagattgt ttgctttaaa aacacttatt atatctaaaa
     1861 tgtcgttgtt caaaagccga tgaacatcgg cttttgttta ttgaaattag aatatctgct
     1921 atggcaccat taccagcctg aatcctaaat tgtaatattt atttgcaggt ctatcactgc
     1981 gtctggcagt taccgtacag tcgcttgcct cgtaatcaca tacacctcct ctaaacacac
     2041 gagttgttcc agagcttggt ccagcagggt tcgtttctgc agcttcgcta tacacccaat
     2101 acctatcttg t
//
LOCUS       DAOPUR010000279         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_56520_length_2109_cov_3.065239, whole genome shotgun sequence.
ACCESSION   DAOPUR010000279 DAOPUR010000000
VERSION     DAOPUR010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56520_length_2109_cov_3.065239"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PKN90_05415"
     CDS             complement(<1..785)
                     /locus_tag="PKN90_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNZ85751.1"
                     /translation="MIVEKIEQFALSKKDHPKAFFSKIGIVGCGKEGSMLATLAAING
                     IEVVFMEPTEEKIHNAYARIESRLDERVDNWGLTPGEKRAILARVKGTVTYETFKGCD
                     FVIESIRYDDNTGERSTNLRREVFLNLENVLSKDAIIATNISTPIITELATELKHKDR
                     CIGMHVMSNVPDARILEVVKSLYTSDDVYTKVCHFAHLIKHEVILVAESAGLVSMRIF
                     FVMLNEACDMLMEGVSTVEDIDRVMRIGFGNRQGVFRTADQMG"
     gene            complement(802..1797)
                     /gene="pta"
                     /locus_tag="PKN90_05420"
     CDS             complement(802..1797)
                     /gene="pta"
                     /locus_tag="PKN90_05420"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNZ85752.1"
                     /translation="MKLLEKIFQQAKNNLRCIVLPESFEKRTLRAADIVLQQQIAHIQ
                     LIGNKASILTTAKELGLAHIHQARFFDPETDPKMDEYSQLLYSLRKAKGMTLEEARVL
                     AKNPLYLSVLMIKNGDVDGELAGAANSTGNVLRPALQIIKTKPGVSVVSGVFFMLFDD
                     TSIGEDGVMVFADCAVTPNPSAIQLAEIAVVTAETTQIIGGFTPRVALLSFSTKGSAK
                     HEMVDKVVTATQLAQQQAPTICIDGELQVDAAIVPSVGASKAPNSLLKGNANVLIFPT
                     LETGNIAYKLVQRLAGAEAIGPVLQGMGAPINDLSRGCSVEDVVRMIAITANQAR"
     gene            complement(1882..>2109)
                     /locus_tag="PKN90_05425"
     CDS             complement(1882..>2109)
                     /locus_tag="PKN90_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ85753.1"
                     /translation="IIINKLADDIGLDKQIGPHTFRHSFATHLLEGGANLRVIQEMLG
                     HESILTTEIYTHVDVHFLRETIMSYHPRNKK"
BASE COUNT          613 a          469 c          321 g          706 t
ORIGIN      
        1 atacccattt ggtcggctgt tctaaacacc ccttgtctat ttccaaagcc aatcctcatt
       61 accctatcta tatcttctac ggtagacact ccttccatca acatatcaca agcttcattg
      121 agcataacaa agaaaatgcg catactcact aaccctgctg attcagcaac caaaattact
      181 tcatgtttaa ttaaatgagc aaaatgacat actttggtat atacatcatc actggtatat
      241 aaacttttta ccacctccaa aatacgagca tctggcacat tgctcattac atgcattcct
      301 atgcatctat ctttatgttt gagttcggta gctaattctg taataattgg agtagatata
      361 ttggtggcta taatagcatc tttagacaac acattttcta agttcaagaa aacttctcgt
      421 ctcaaatttg tacttctttc tcctgtatta tcatcatacc gaatagattc aattacaaaa
      481 tcacaacctt taaacgtttc gtacgttact gttcctttta cacgagcgag tattgctctt
      541 ttctcacctg gcgtaagccc ccaattatca acgcgttcgt ctaatcggct ttcgatgcgt
      601 gcataagcat tatggatttt ttcttccgta ggttccataa acactacttc tatcccatta
      661 atggctgcca atgtcgccaa catacttccc tctttaccac aacctacaat tccgattttt
      721 gaaaagaaag ctttgggatg atctttctta cttagggcaa attgttcaat tttttcaact
      781 atcatatttc ttatccttaa attatctagc ctgatttgct gtaatagcaa tcatccgcac
      841 aacatcctct actgagcaac ctcttgacaa atcattaatt ggagcaccca ttccttgaag
      901 cactggacca atagcttctg ccccagctaa acgttgcact aatttgtatg caatatttcc
      961 agtttccaat gtagggaata tcaatacatt tgcattccct tttaacaaac tgttgggtgc
     1021 tttagaagcc ccaaccgacg gaactatggc agcatctacc tgcaactctc catctataca
     1081 tattgttgga gcttgctgtt gagctaattg tgtagcagtt actactttgt ctaccatttc
     1141 atgtttggca cttcctttgg ttgaaaaact caacaaagca acacgcggag taaaccctcc
     1201 gatgatttga gttgtttctg ccgttacaac agctatttct gctaattgta tagccgaggg
     1261 gttaggagta acggcacaat cggcaaacac catcacaccg tcttcaccta tgcttgtgtc
     1321 atcaaatagc ataaaaaata cgccagatac aacacttacc cctggtttag tttttataat
     1381 ttgtaatgct ggtcgcaata catttcccgt tgaatttgcg gcaccagcca attctccatc
     1441 tacatctcca ttttttatca tcaacaccga gaggtacaaa ggattctttg ctaatacccg
     1501 agcttcttct aacgtcatcc cttttgcctt acgtaacgaa tacaaaagct gactatattc
     1561 gtccatcttg gggtctgttt cgggatcaaa aaatctcgct tggtgaatat gggctaaacc
     1621 cagctctttg gctgttgtaa gaatactagc tttattacca attaactgga tatgtgcaat
     1681 ttgttgttgc aagacaatat ctgcggcacg caatgtgcgt ttttcaaatg attcgggcaa
     1741 tacaatgcaa cgtaaattgt tttttgcttg ttgaaatatt ttttctaata acttcatata
     1801 aaacacctta ggagtaacaa attgtttttt aaaatagaag taccaaagtt acaagaataa
     1861 tagcattgaa acaagcattt tttatttttt atttctaggg tgatatgaca ttatcgtttc
     1921 tctcaaaaaa tgcacatcta catgcgtata aatttcggtt gttagtatac tttcatgtcc
     1981 taacatttct tgaataaccc tcaaattagc tcctccttcg agtaaatgag tagcaaaact
     2041 atgccgaaaa gtatgtggac caatttgttt gtctaagcct atatcatctg ctaatttatt
     2101 aatgatgat
//
LOCUS       DAOPUR010000280         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_56988_length_2097_cov_3.177767, whole genome shotgun sequence.
ACCESSION   DAOPUR010000280 DAOPUR010000000
VERSION     DAOPUR010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56988_length_2097_cov_3.177767"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PKN90_05430"
     CDS             <1..905
                     /locus_tag="PKN90_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ85754.1"
                     /translation="FDEMAPFIHPDNIHFYFSSNGHPGLGGFDVFQAKKDSLEAWNTI
                     SNMGYPINTFGDEIGFIISSSGEKAYLSSNQHIDIDNQHLNRMIYEITLPHSLRPETM
                     QTVKGVVIDNKTNQPLQANIEIFDTHSQKTISRTTSDNKTGTFTAIIPQKSQVGMFVK
                     KDQYLVYSEQIDSTSSDFYTIKLEKIEKGRSFVLRNIYFDFDSAELLSNSYAELHRLL
                     EFLQSNPTLNITLIGHTDNFGNKEYNRSLSIQRARSVYNYLLKKGIDKSRLQFEGKGA
                     DMPTEDNNTDFGRSRNRRIEFVIH"
     gene            complement(908..1822)
                     /locus_tag="PKN90_05435"
     CDS             complement(908..1822)
                     /locus_tag="PKN90_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNZ85755.1"
                     /translation="MSWKDYFYFTKQEKRGLSVLIVMLIVLIGTYVGLPYFISNPVVM
                     DDEEVKLQIADFEKTLQEDSASFVYKKQHWSKNIDSTTSILQVFDPNHADSALFLKLG
                     IKPYVISRILKYRAKGGVFRSPTDFSKIYGLPKEMYERLLPYIQIKPIAHDKQLVESK
                     ESLPVTTSEITFVDINKADTTMLKTVKGIGSVLSKRIVTYREKLGGFISVEQLKEVYG
                     ITDEVYQKIAPQLTVSQSYTKKISINQATVHQLKQHPYISFYQAQSIYEYRMGKRTKR
                     IENIEEIPFNRDINSDFIEKIKPYVTCE"
     gene            complement(1825..>2097)
                     /locus_tag="PKN90_05440"
     CDS             complement(1825..>2097)
                     /locus_tag="PKN90_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNZ85756.1"
                     /translation="ASLSLTLLFPVIFDKTLFEWLDFITATILLPLGGVAVSLFVGWY
                     LDKRILLDVLQQEGTWKFRFFKTYIFLLRYLAPVCIMLVFVHNLFA"
BASE COUNT          714 a          383 c          329 g          671 t
ORIGIN      
        1 ctttcgatga aatggctcct tttattcatc cagacaatat tcacttctat ttttcatcta
       61 acgggcaccc agggctagga ggatttgatg tatttcaagc taaaaaagac tcactagaag
      121 cttggaatac tatcagtaat atgggttatc ctattaatac gtttggcgat gaaataggat
      181 ttattatctc ctcttcagga gaaaaagcat atttatcatc aaatcaacat attgacattg
      241 ataatcaaca tctaaataga atgatttatg aaataacttt gcctcattcg ttgcgaccag
      301 aaacaatgca aacagtaaaa ggagttgtta ttgacaataa aacaaaccaa cccttgcaag
      361 ccaatatcga aatctttgat actcattcgc aaaaaacaat ttcacgaact acgtctgaca
      421 ataaaacagg cacctttaca gccattattc cccaaaagag tcaagtggga atgtttgtaa
      481 aaaaagacca atacttggtg tattccgaac aaattgattc tacttcttca gacttttata
      541 caataaaact tgaaaagatt gaaaaaggga gaagctttgt tcttcgcaat atttatttcg
      601 attttgactc agccgaacta ctctccaatt cgtatgctga attacatcga ttgcttgagt
      661 ttcttcaatc aaaccctacc ttaaacatta cattaattgg tcatacagac aatttcggga
      721 acaaggagta taatcgaagt ttatctatac aacgtgcccg ttcagtatat aattatctac
      781 taaaaaaagg gatagataag tcgcgattac aatttgaagg caaaggagcc gatatgccta
      841 ctgaagacaa taatacagat tttggtagga gtagaaacag aagaattgaa tttgtgatac
      901 actaatttta ttcacatgta acatacggct taattttttc gatgaaatcg ctatttatat
      961 cccgattaaa tggaatttct tcgatgtttt ctatccgttt agttcgctta cccatcctat
     1021 actcataaat gctttgtgct tgataaaacg atatatacgg atgttgtttt agttgatgga
     1081 cagtcgcttg attaatagaa atcttcttag tataagactg tgatacagtt agttgcggag
     1141 caattttctg gtatacttca tctgttatcc catatacctc ttttagttgc tcaacagaaa
     1201 taaatccgcc aagtttttct ctatacgtaa cgattcgttt tgataataca gagccaatac
     1261 cctttactgt tttcagcatt gttgtatcag ctttattgat atctacgaat gttatttcac
     1321 tcgttgttac aggtaatgat tcctttgatt ctactagttg tttgtcgtgt gcgatgggtt
     1381 taatttgaat atatggcaac aatcgttcat acatttcttt tggcaacccg tagattttag
     1441 aaaagtcagt aggcgatcga aataccccac ctttagctct atactttaaa atacgtgata
     1501 tcacataggg ttttatgccc agtttcagga ataaagctga atcagcatgg tttgggtcaa
     1561 atacttgcag tatactcgtc gtagaatcaa tatttttgct ccaatgttgt tttttatata
     1621 caaatgaagc tgaatcttct tgtaatgttt tttcaaaatc tgctatttgt aacttcactt
     1681 cctcatcatc cataaccaca ggattagaga tgaaatatgg taaaccaaca tatgtaccaa
     1741 ttaatacaat aagcatcact atcaatacag acaaccctcg cttttcttgt ttcgtaaaat
     1801 aaaaataatc tttccaagac atgtttacgc gaatagatta tgaacaaaaa caagcataat
     1861 acatacagga gctaagtagc gtaacaaaaa gatatacgtt ttaaaaaagc gaaatttcca
     1921 agtcccctct tgttgtaata catccaacag aatacgctta tctagatacc agccaacaaa
     1981 taatgacaca gcaacccctc ctaatggcaa caaaatagta gccgtaataa aatctaacca
     2041 ttcgaagagc gttttatcaa aaataacggg aaataataag gttaaagata acgaagc
//
LOCUS       DAOPUR010000281         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_57478_length_2084_cov_2.806309, whole genome shotgun sequence.
ACCESSION   DAOPUR010000281 DAOPUR010000000
VERSION     DAOPUR010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57478_length_2084_cov_2.806309"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PKN90_05445"
     CDS             <1..658
                     /locus_tag="PKN90_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNZ85757.1"
                     /translation="NPVKYKIPEGDEITLVAPLSHIIFYADNKTHFGIFHTNFTLQES
                     NYIALYDRDQTTCLDSVVYPLTMPANFSYGRLKDGEPEWGMLPKTSPANNNAFVDKGE
                     TSKVFAQQDPYGVVLTIASMSVVFLALLLLYALFSFTAKFSIYVTKERIRKTAKGEKS
                     ETIVETHIPAEVYASIAMAIHEYQEEIHDVEDTILTIQRVAKTYSPWSSKIYGLRNFL
                     "
     gene            751..1197
                     /locus_tag="PKN90_05450"
     CDS             751..1197
                     /locus_tag="PKN90_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNZ85758.1"
                     /translation="MKEYNFIINGTKYKVGIQSVEDNIAEVEVNGTPFYVEMEKPLVK
                     KGIKPLAKRAVYTHDETTVSSPSQPVVKPVVKVKTGALLSPLPGVITAISVNVGDTVK
                     IGQKVLALEAMKMENNINADKDGKVLEIKVSKGDSVLEGTELIIIG"
     gene            1202..>2084
                     /locus_tag="PKN90_05455"
     CDS             1202..>2084
                     /locus_tag="PKN90_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ85759.1"
                     /translation="MIQQDNLLSFLLNSINKIILSTGFANVEIGNIIMIIIGIMFIYL
                     AIKKGWEPMLLVPIGFGMIVGNIPFLEGSDIGIKEEGTVLNILYGGVRYGWYPPLVFL
                     GIGAMTDFASLISNPKLLLIGAAAQFGIFGAYALALAFGFPANEAGSIGIIGGADGPT
                     AIFVTSILSPHLLGAIAVSAYSYMALVPVIQPPVMRLLTTKKERLIHMKKPRAVTQTE
                     KVVFPIMGLLLTTFIVPSGLPLLGMLFFGNLLKESGVTNRLADTAKGPMIDIVTILLG
                     FTVGASTQAAFFLTTKSI"
BASE COUNT          694 a          296 c          395 g          699 t
ORIGIN      
        1 taatcctgta aaatacaaaa tacctgaagg agatgaaata acacttgttg ctccattatc
       61 tcatataata ttttatgcag ataataaaac tcattttgga atatttcata caaactttac
      121 attacaagaa agtaattata tcgctttgta tgatagagat caaacaacgt gtttagattc
      181 ggtagtctat cctcttacta tgcctgctaa tttttcctat ggacgattga aagatggtga
      241 acctgaatgg ggtatgttgc ctaaaacatc tccagctaat aataatgctt ttgtagataa
      301 aggagaaact agtaaggttt ttgcacaaca agatccatat ggagttgtat taactatagc
      361 ttctatgtct gttgtgtttc ttgcactact actactgtat gcattatttt cttttacagc
      421 aaagttttct atatacgtaa ccaaagagcg tattcgtaaa acagctaagg gagaaaaaag
      481 tgaaaccatt gttgaaacac atattccagc agaagtatac gcgtctattg ccatggctat
      541 acacgaatat caggaagaaa tacatgatgt tgaagatact attttgacta tacaacgtgt
      601 tgctaaaacg tattctcctt ggagttctaa aatatatggg ttgcgtaatt ttttgtaaaa
      661 aaatcaacaa gctgtataga aaaaataact ggtaaatata caatcagtta tatcgtttac
      721 tatatatgaa ttttttaata aataaaatag atgaaagaat ataattttat tataaatggt
      781 accaaatata aagttggtat acaaagcgta gaggacaata ttgcagaagt agaggttaat
      841 ggtactcctt tttacgtaga aatggaaaaa cctcttgtta aaaaaggtat taaacctctt
      901 gcaaaacgtg cagtatatac gcatgatgaa acaacagtga gctctccttc tcaacccgta
      961 gtaaaacctg tagtgaaagt aaaaacagga gcacttttat ccccattacc tggagttatt
     1021 acagctatta gtgtcaatgt aggtgataca gttaaaattg ggcaaaaagt attagctcta
     1081 gaggcaatga aaatggaaaa caatatcaat gccgataaag atggcaaagt attggaaata
     1141 aaagttagta aaggggattc tgttttagaa ggaactgaat taattataat aggatagaat
     1201 aatgatacag caagataatt tactttcatt tttgctcaat agtatcaata agattatttt
     1261 gagtacaggg tttgcaaatg tagaaatagg taatattatc atgattatta tcggaatcat
     1321 gtttatctat ttagctatta aaaaaggatg ggaaccaatg cttttagttc ctattggttt
     1381 tggtatgatt gttggaaaca ttcctttttt agaaggttca gatattggta taaaagaaga
     1441 aggtacagtt ttaaatattc tttatggtgg ggttagatat ggttggtatc ctcctttagt
     1501 atttttaggt attggagcaa tgactgattt tgcatctctt atctctaatc caaaattgtt
     1561 attaattgga gctgctgcac aatttggtat atttggtgct tatgcactgg ctttagcgtt
     1621 tggatttcct gctaatgaag caggttcaat aggtattatt ggtggtgctg atggtccaac
     1681 agctattttt gtaacgtcta tattgtcacc acatttattg ggagcaatag ctgtgtcagc
     1741 ctactcgtat atggctttag tgcctgttat tcaaccccca gtaatgaggt tgcttacaac
     1801 caagaaagag cgtcttattc atatgaaaaa accaagggct gttactcaga cggaaaaagt
     1861 ggtttttcct ataatgggat tattattaac gacttttatt gttccatcag gattgccatt
     1921 attggggatg ctattttttg ggaatttgtt aaaagagagt ggggtaacca atcgattagc
     1981 agataccgcc aaaggtccta tgattgatat tgttacaatc ttgttaggtt ttactgttgg
     2041 agcatccact caagcggcgt ttttcctgac gactaaatca attt
//
LOCUS       DAOPUR010000282         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_57523_length_2083_cov_2.821992, whole genome shotgun sequence.
ACCESSION   DAOPUR010000282 DAOPUR010000000
VERSION     DAOPUR010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57523_length_2083_cov_2.821992"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..729)
                     /locus_tag="PKN90_05460"
     CDS             complement(10..729)
                     /locus_tag="PKN90_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNZ85760.1"
                     /translation="MARNSFFEFKQFRINQENCAMKVGTDGVLLGAWTPIPLSTTRIL
                     DIGTGSGLLALMLAQRTHAHIDAVEIDTLAAQQAKENFISSPWSNRLQIYETDFQSFA
                     KNELHKYDVIVSNPPYFHNSLHTPNRQRTIARHSDSLTLDELVDGVEKLLQPWGYFCV
                     ILPTHESETVAKKALLANLYCTQKTFVYPTPTSPAKRIMQLFSKKETTPRTQHLVIEQ
                     EQRHQYTKEYSELTKDYYLNF"
     gene            940..2010
                     /gene="carA"
                     /locus_tag="PKN90_05465"
     CDS             940..2010
                     /gene="carA"
                     /locus_tag="PKN90_05465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNZ85761.1"
                     /translation="MQKSKAAKLVLENGVVFHGYSFGYEQSKAGEVVFNTAMMGYPES
                     LTDPSYSGLILAFTFPLIGNYGVPSSNVTHQIADFFESEKIHASGVVVSTYSFEYSHW
                     NAKQTLSDWLIENKVPGIYGVDTRELTKVLREKGAMKGKIVIEDADVDFIDSNAINQV
                     NIVSCKEVMHYGNGKKKVVLVDCGVKHNIIRCLLKRDVTLIRVPWNYDFNTLEFDGLF
                     LSNGPGNPSTCAETVEHIRKAMKRNVPIFGICMGNQLLAKAGGAETYKLKYGHRSHNQ
                     PVRMVGTNRCFVTSQNHGYAVDNTTLSEEWEPLFQNMNDDTNEGLKHKSKPFFSVQFH
                     PEAASGPTDTEFLFDEFIKMID"
BASE COUNT          655 a          351 c          414 g          663 t
ORIGIN      
        1 ttttcctctt caaaaattta gataataatc ttttgtaagt tcactatatt ctttggtata
       61 ctgatgtctt tgttcttgtt caattaccaa atgctgtgta cgcggggttg tttctttttt
      121 cgaaaatagt tgcatgatgc gtttggctgg gctcgtaggt gttgggtaaa caaacgtttt
      181 ttgtgtgcaa taaagatttg ccagcaaagc ctttttagct actgtttcac tttcatgtgt
      241 aggtaatatg acacaaaaat atccccaagg ttgtagtaat ttttcaacgc catcaactaa
      301 ttcgtctagc gtaagcgaat cactatgtcg agctattgta cgttgtctgt ttggagtatg
      361 caatgaattg tgaaaataag ggggatttga tacaataaca tcatatttat gtaattcgtt
      421 tttggcaaat gattgaaaat cggtttcata aatctgtaat cgattgctcc aaggagaaga
      481 tatgaaattt tctttggctt gttgagctgc caaggtgtct atttctacgg catcaatatg
      541 ggcatgtgtg cgttgagcga gcatcagagc aagtaatcca cttcctgttc caatgtccaa
      601 tatacgggta gtgctaagtg gtatgggagt ccatgcccca agcagcacac catctgtgcc
      661 aactttcata gcacaatttt cttggttaat tctaaattgt ttaaactcaa aaaacgaatt
      721 tcttgccata aatatgttaa aaatgcaaat atacagtaaa cttttcatat gctaaacagt
      781 gtctaactcc catcaatatg gctatcgaac aaatcaattt attattcgtg taaaaatgtt
      841 gaaaaatagc aaaatcctgt ttgtttggaa tgctaaaata ttgtatcttt gtgcaatttt
      901 tgagaactac tcattgtaac atatttatat tagtaaaaca tgcaaaaaag caaagctgcc
      961 aagttggtgt tagagaatgg agtcgttttt catgggtact ccttcggtta tgaacagtcg
     1021 aaagcaggag aggtggtttt taacactgcc atgatgggct atcccgaaag tttaacagac
     1081 ccttcctatt cgggtctaat cttagcattt acattccctt taatcggcaa ttatggtgtt
     1141 ccttcttcca atgtaaccca tcaaattgcc gatttttttg agtccgaaaa aattcatgct
     1201 tcgggcgtag tagtgtcaac atattcgttt gaatacagcc actggaatgc aaaacaaacc
     1261 ctaagcgatt ggttgattga aaacaaagtg ccagggattt atggcgttga tacgcgtgaa
     1321 ttgacaaaag tattgcgcga aaaaggtgca atgaaaggaa aaatcgttat cgaagatgct
     1381 gatgttgatt ttatcgattc aaatgcaatt aatcaggtaa acatcgtgtc gtgcaaagag
     1441 gtaatgcact acggaaatgg aaagaaaaaa gtagtattgg tcgattgtgg tgtaaaacat
     1501 aacatcattc gttgcttatt aaaaagagat gtaacgctca ttcgtgtgcc ttggaattat
     1561 gattttaaca ccctcgaatt tgatggctta tttttgtcta atggtcctgg caacccatct
     1621 acatgtgctg aaactgtaga acatatacgc aaagcaatga aacgaaatgt acccatattt
     1681 ggtatttgca tgggcaatca attgttggca aaagcgggtg gtgctgaaac atataaacta
     1741 aaatatggtc atcgtagcca caaccaaccc gttcgtatgg tggggacaaa tcgttgtttt
     1801 gtaacttcac aaaatcatgg atatgctgtt gataacacta ccttgagtga agaatgggaa
     1861 cctcttttcc aaaatatgaa tgatgatacc aacgaaggtc taaaacataa atcaaaacct
     1921 ttcttttcag tccaatttca ccccgaagca gcaagtggac ctactgatac agagttttta
     1981 tttgacgaat ttattaaaat gattgactaa tgaacgagaa tttaatacca ggaataaaaa
     2041 aagttattgt tttaggctca ggagcactca aaattgggga agc
//
LOCUS       DAOPUR010000283         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_57569_length_2082_cov_2.703503, whole genome shotgun sequence.
ACCESSION   DAOPUR010000283 DAOPUR010000000
VERSION     DAOPUR010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57569_length_2082_cov_2.703503"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2082
                     /gene="metH"
                     /locus_tag="PKN90_05470"
     CDS             <1..>2082
                     /gene="metH"
                     /locus_tag="PKN90_05470"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNZ85762.1"
                     /translation="KYVEAGADIISTNTFNSNRISMSDYGMENLVYELNYEAAKLAKS
                     VTTHYTTSSKPLFVAGAIGPTNKTLSMSPKVEDAGYREISFEQLATVYAEQVEALMAG
                     GVDVLLVETIFDTLNAKAAIYAIQDVFARLGKEIPIIISGTLLDKSARTLSGQTLEAF
                     VISVSHAKPLAIGLNCSTGASDMLPHIIELGKLTDTFVSAYPNAGFPNELGKYDETPQ
                     QMSNQIEEFLSHQAVNIIGGCCGTTPEHITRFAQLAASATPHQRTTLEPFTRLSGLEP
                     MVIKKENNFVNIGERTNVAGSKKFARLIADKKYEEAIQIARQQVEGGAQIIDICMDDA
                     MLDSAASMTDFLQLVATEPSVAKVPIMIDSSKWEVLEAGLRCVQGKSVVNSISLKEGE
                     EVFLNHAKKIRQYGAAVVVMAFDEQGQADTFEKRIAVCQRAYTLLTNIGFPAKDIIFD
                     PNILAIATGLEEHANYAIDFIRTVEWIKKELPYAKVSGGVSNLSFSFRGNNTVREAMH
                     SVFLYYATQKGMDMGIVNPEMLEIYSNIPADLLELTEDVIFNRRADATERLINYANEV
                     KSNTSKETKIDEWRTKSLQERLGHAIIKGIYDYLDTDLTEARTQYAKALEIIEGPLMD
                     GMNIVGDLFGSGKMFLPQVVKSAQVMKKAVAFLLPYIEAEKDSNSQNAGKIVMATVKG
                     DVHDIGKNIVGV"
BASE COUNT          695 a          396 c          439 g          552 t
ORIGIN      
        1 caaatacgta gaagcaggtg cggatattat ttcaaccaat acatttaatt ccaatcgaat
       61 ctctatgtcc gattatggga tggaaaacct ggtgtacgag ttaaattatg aagcagcaaa
      121 actcgcaaaa tcagttacca cgcactatac aacaagctca aaaccattgt ttgtagcagg
      181 agctattgga ccgaccaata aaaccctgtc gatgtctccc aaagtagaag atgcaggcta
      241 tagggaaata tcgtttgaac aattggcaac ggtatatgct gagcaggtag aagcattgat
      301 ggcaggagga gtagatgtgt tgttagtcga aacaattttc gacacgctca atgcaaaagc
      361 agccatctat gccatacaag atgtatttgc acgcttgggc aaagaaattc ccatcattat
      421 ttcaggtacc ttactcgaca aaagtgcccg tacactaagt ggacaaacgc tcgaagcttt
      481 tgtaatatct gtgtctcacg ctaaaccttt ggctattggc ttaaactgtt cgactggtgc
      541 atctgatatg ttgcctcata tcattgaatt aggaaaactg acagatacgt ttgtgagtgc
      601 atatcccaat gcaggtttcc cgaacgaact gggtaagtat gatgaaacac ctcagcaaat
      661 gtctaaccaa attgaagagt ttttatccca tcaagcggtt aatattattg gaggatgttg
      721 tggtactact cccgaacaca ttacccgttt tgcacaactt gcagctagtg ctacacctca
      781 ccaacgtact actttggaac cattcactcg tttgagtgga ttagaaccaa tggttatcaa
      841 aaaagagaat aattttgtaa acattggcga acgtactaac gtggcagggt caaaaaaatt
      901 tgcacgctta atagccgata aaaaatacga agaagccata caaattgctc gtcagcaagt
      961 agaaggtgga gctcaaatta ttgatatttg catggacgat gctatgctcg actccgctgc
     1021 tagtatgact gattttttgc aattagttgc caccgaaccc tcagtagcca aagtgccaat
     1081 tatgattgac tcctcaaaat gggaggtatt agaagctggt cttagatgcg tacaaggcaa
     1141 atcggttgtt aattctattt cgctaaaaga gggagaagaa gtatttctta atcacgcaaa
     1201 aaaaattcgt caatatggag cagccgtggt agtgatggca tttgacgaac aaggtcaagc
     1261 cgatacattt gaaaaacgca tagctgtatg ccaacgtgcc tatacactat tgactaacat
     1321 aggttttcct gccaaagaca tcatttttga tcccaatatc ttagctattg ctactggact
     1381 tgaggaacat gctaattacg caattgattt tattcgtacc gtagaatgga taaaaaaaga
     1441 acttccttat gccaaagtaa gtggtggagt aagcaaccta tccttctcat ttagaggcaa
     1501 taatacggtg cgagaagcca tgcattcggt atttttatac tatgctacgc aaaaaggcat
     1561 ggatatggga atagtaaatc cagaaatgct cgaaatatac agcaacatac cagccgactt
     1621 gcttgaacta acagaagatg tgatttttaa tcgcagagcc gatgctacag aacgattgat
     1681 aaactatgcc aacgaagtta aaagtaacac cagcaaagaa acaaaaatag atgaatggcg
     1741 taccaagtca ttacaagaac gtttaggaca cgctattatt aaaggcattt acgattattt
     1801 agataccgac ctcacagaag ctcgtacaca atatgccaaa gcattagaaa taatcgaagg
     1861 tccgctcatg gacggaatga atattgtggg tgatttgttt ggttcgggaa aaatgtttct
     1921 gccacaagtg gtaaaaagtg cacaagtaat gaaaaaagcc gttgcctttt tactccccta
     1981 tatcgaggca gaaaaagaca gcaacagtca gaatgctggc aaaattgtga tggctacagt
     2041 aaaaggggat gttcatgata ttggtaagaa cattgtagga gt
//
LOCUS       DAOPUR010000284         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_57680_length_2079_cov_3.755435, whole genome shotgun sequence.
ACCESSION   DAOPUR010000284 DAOPUR010000000
VERSION     DAOPUR010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57680_length_2079_cov_3.755435"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKN90_05475"
     CDS             <1..406
                     /locus_tag="PKN90_05475"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNZ85763.1"
                     /translation="QETNDLTKAVHYCVENKLYNVVIMGATGKREDHTLGNISLLALY
                     HSLLHQVVMISDYGIFSAIDKTTVFSSYEHQQVSIFSILPTCPISVAGLRYPIIQRPL
                     EMWWQGTLNESLGTSFTVQLHDKGLCIVFREW"
     gene            complement(414..776)
                     /locus_tag="PKN90_05480"
     CDS             complement(414..776)
                     /locus_tag="PKN90_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNZ85764.1"
                     /translation="MKKYVYIALGTISLGLGIVGIFVPLLPTTPFLLLTAALYCHSSD
                     KLYNKLMNHKTLGPYIHDFRTHKSIPLKTKIISISLVWITIGYSIMCVVPLLFVKILL
                     AAIAIGVTIHILSFKTKK"
     gene            complement(773..1741)
                     /gene="fmt"
                     /locus_tag="PKN90_05485"
     CDS             complement(773..1741)
                     /gene="fmt"
                     /locus_tag="PKN90_05485"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNZ85765.1"
                     /translation="MTKEELRIVYMGTPEFAVAPLQSLLANQYNVVGVVTMPDKPAGR
                     GHKLQASAVKQVAIEHNLPILQPEKLKDPIFLEALRSLNADLFIVVAFRMLPEVVWNM
                     PRMGTFNLHASLLPQYRGAAPINWALIRGEKETGVTSFFLTHEIDTGDILLQRKIAID
                     DTDNAGSLHDKLMQLGAETVVETAAYILSGNTSKTPQNELMNGQQIHLAPKIFKETCR
                     INWSLSCEEIKNFVRGLSPYPAAYTEGLDAKGAPFSAKILHVSTQVCTPEARIGTVST
                     DNKTTLKIACADGWVIIDSIQVAGKKAMQTEELLRGFHLLVPQQLT"
     gene            complement(1822..>2079)
                     /locus_tag="PKN90_05490"
     CDS             complement(1822..>2079)
                     /locus_tag="PKN90_05490"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNZ85766.1"
                     /translation="EQSRLLKQQQELQDLDARLTKELMSKQQKMNEQLKASIDSIILE
                     FNKDNKYHLILSNTGNDNILYGNKAYNITNEILKLMNVEKE"
BASE COUNT          614 a          374 c          391 g          700 t
ORIGIN      
        1 ccaagaaacc aatgatttaa ccaaagctgt acattattgt gtggaaaata aactatacaa
       61 tgtagttatt atgggtgcta caggcaagcg tgaagaccat actttgggta atatttcgtt
      121 gttggcgttg taccattcgc tgttacacca agtagtgatg ataagtgatt atggaatatt
      181 ttccgccata gataaaacaa cagttttttc tagttatgag catcagcaag tctccatttt
      241 ttccatactt cctacctgtc ctatcagtgt ggcgggatta cgatatccaa ttatacaacg
      301 accgctcgaa atgtggtggc agggtacact caacgaaagt cttggtacat cttttactgt
      361 tcagttacac gacaagggtt tatgtattgt atttagagag tggtaaagaa tgcttatttt
      421 tttgttttga aagacaaaat atgtattgtt acgccaatag ctatagcagc taacaatatt
      481 tttacaaaaa gtagaggaac aacacacata atcgaatagc caatggtaat ccaaaccaat
      541 gatatggata tgattttcgt ttttaatgga atacttttgt gtgtgcgaaa atcatgaata
      601 tagggaccta aggttttatg attcattaat ttgttatata gcttatccga actatggcaa
      661 tacaaagctg ctgtaagcaa caaaaaagga gtagtaggca ataagggtac aaagatgccc
      721 actatgccta gccctaacga gatggtacct aatgcaatat atacgtattt tttcatgtaa
      781 gttgttgtgg tacaagcaag tggaatccac gtagtaattc ttctgtttgc attgcctttt
      841 tccctgctac ttgaatgctg tcaatgatta cccaaccatc agcacacgca atcttgagtg
      901 tcgttttgtt atccgtactt accgttccaa tacgtgcttc aggggtgcaa acttgtgtgc
      961 taacgtgtaa tattttagcc gaaaaaggag cccctttcgc atccaagcct tctgtatacg
     1021 ctgcagggta cggagataaa cctcgtacaa agttttttat ttcttcgcac gacaatgacc
     1081 aatttatgcg acaagtttct ttaaatattt tcggggcaag gtgtatctgt tgtccgttca
     1141 ttaactcatt ttgaggcgtt ttcgaagtat taccagatag aatataggct gccgtttcta
     1201 ctaccgtttc agctcctagt tgcattaatt tatcgtgtag cgaacccgca ttatccgtat
     1261 cgtcgatagc tatttttcgt tgcaacagga tatctcctgt atcaatttcg tgggttaaga
     1321 aaaaactagt aacgccagtt tctttctctc ctcttatcag agcccaattg atgggagctg
     1381 ctccacggta ctgtggtaat aaggaggcgt gtaagttaaa agtgcccatt cgaggcatat
     1441 tccatacaac ttctggtaac atacgaaaag ccactacgat aaataaatca gcattcaaag
     1501 agcggagtgc ttccaaaaaa attgggtctt ttagtttttc tggttgcaaa atgggaagat
     1561 tatgttcaat ggctacttgt tttacggcag atgcttgtag cttatgtcct ctaccagcag
     1621 gtttgtcggg catagtaacg actcctacca cattatactg attagccaac aagctttgta
     1681 gaggagcaac agcaaattct ggtgttccca tatatacgat gcgtaattct tctttcgtca
     1741 tattgtataa atagtagata atgaaaaaag ccactcttat tgtgataata agagtagctt
     1801 tgatgcgacg taaattatac cttattcttt ttcaacattc attaatttca aaatttcatt
     1861 ggtaatattg tatgctttat ttccatataa aatattatca ttgccagtat tacttaaaat
     1921 tagatgatac ttattgtctt tattaaattc taaaataatg gaatcaatag acgcttttaa
     1981 ttgctcattc attttttgtt gtttactcat caattctttg gttaaacgtg catcaagatc
     2041 ttgtagctct tgttgttgtt taagtaaacg tgattgttc
//
LOCUS       DAOPUR010000285         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_58277_length_2066_cov_2.641969, whole genome shotgun sequence.
ACCESSION   DAOPUR010000285 DAOPUR010000000
VERSION     DAOPUR010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58277_length_2066_cov_2.641969"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PKN90_05495"
     CDS             complement(<1..690)
                     /locus_tag="PKN90_05495"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNZ85767.1"
                     /translation="MKVALITGITGQDGSYLAEFLLEKGYEVHGVLRRSSSFNTERIE
                     HLYLTEWVRDMKKKRAINLHYGDMTDSSSLVRIIQDVKPDEIYNLAAQSHVKVSFDVP
                     EYTAECDAVGTLRLLEAVRILNLEKECKIYQASTSELFGLVQEIPQKETTPFYPRSPY
                     GVAKQYGFWITKNYRESYGMFAVNGILFNHESERRGETFVTRKITLAAARIAQGMQDK
                     LYMGNMDSLSYT"
     gene            complement(941..1276)
                     /locus_tag="PKN90_05500"
     CDS             complement(941..1276)
                     /locus_tag="PKN90_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85768.1"
                     /translation="MKVINNVITEGLSGKVEQSKYCFRRHYGSTFLTLKSIPSGEETP
                     QQRLVKSWFTEAQRLANVDMGDDAKRRLWEQNLKKTKYKTPKGRAFAHYFQQLKAGAI
                     TVPEITEEP"
     gene            complement(1623..>2066)
                     /locus_tag="PKN90_05505"
     CDS             complement(1623..>2066)
                     /locus_tag="PKN90_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85769.1"
                     /translation="ERDRAALESRLRQMYHQFTSADWDLKELHELPIEANLDTAIDTE
                     TSKSSSGITLNNDVSKLDFIRVLYVLYELNFFVEESGKKVTKKELFAFLATWLGKETL
                     AHYDKDMTNSWKSDMSAHVAIFDKMRAKMVELATNKNVANYKKIG"
BASE COUNT          658 a          454 c          312 g          642 t
ORIGIN      
        1 cgtatagctc aacgaatcca tattacccat atacaattta tcttgcatac cttgagctat
       61 acgtgcagca gcaagggtaa ttttacgggt aacgaatgtt tcacctctac gttcactttc
      121 gtggttaaat aagatgccat ttacagcaaa cataccatat gattcgcggt aattttttgt
      181 aatccagaaa ccatactgct tagcaactcc atacggagag cgtggataaa aaggtgttgt
      241 ttctttttga ggaatttctt gtactaatcc aaatagttca gatgtagaag cttggtaaat
      301 cttacattct ttttctagat ttagaatgcg tactgcttct aacaaacgta atgtacctac
      361 agcatcacac tcggcagtgt attcgggcac atcaaaacta actttcacat gactttgagc
      421 cgccaagttg tatatttcgt ctggttttac atcttggata atacgtacta aagaacttga
      481 atcagtcata tcgccataat gcaagttaat agctcttttt ttcttcatat ctcttaccca
      541 ctcagtaaga tacaaatgtt cgatacgttc tgtattaaaa gacgatgaac ggcgtaaaac
      601 accatgaact tcatagcctt tttctaataa aaattcagct aaataagatc catcctgacc
      661 tgtaatacct gttattagtg ctactttcat gactgattat aattaaatat gttttaaata
      721 ttatatgaaa ctgcaaagat acgtaaaaaa actcgaaaaa aacaatacaa aaatctatta
      781 ttttcgtaca ctaaaagcca tagacaagga gcgttaagta taacgcgaac aatagacatt
      841 caatagagct tatcaacaga gctacaaatc accaaattca aaataaaaaa tgggaattac
      901 tcaagagtaa ctcccatcct ttcaaccagt caacacaact ctatggctct tctgtaatct
      961 ctggtacagt aatagcacct gctttcaact gttgaaaata atgagcaaaa gccctacctt
     1021 taggagtctt gtacttagtt ttcttcaagt tctgttccca caatcggcgt ttggcatcat
     1081 cgcccatgtc cacgttagct aaccgttgtg cctccgtaaa ccaactcttt accagtcgtt
     1141 gctgaggagt ttcctcgcca ctaggtatgg actttagtgt caaaaatgta ctaccatagt
     1201 ggcgtctaaa acaatacttc gattgctcca ctttaccaga aagaccttct gtaatcacat
     1261 tgttaatcac tttcatagta cacctccttc aacactaaag ttacatactc cgcatccttt
     1321 cataagggcg ttcgccatgt tttgagtctt ggcaaagaca ttaagcagat ttatattcat
     1381 acttaaagag tttaggtcgc taagcgactg ttaataataa aaatgtcaat cacacaacac
     1441 tatctgcgaa actgacagta caaaggtaag tgctttatta tcaacaacaa atagagtttt
     1501 ccctcataca ggaaaactct acataaacag taacaaaacc cacacagtta gttggtgcta
     1561 ccctagcttt tgaaccaaca accctagcct tggtgagatt ttaccctagc tttttgactc
     1621 atttatccaa tctttttata gttagctaca tttttgttag tcgccaattc taccattttc
     1681 gcacgcatct tatcaaaaat agccacatgt gcactcatat cggacttcca actgttggtc
     1741 atatccttat cataatgcgc caatgtctct ttaccgagcc aagtagccaa aaaagcaaat
     1801 aactcctttt tggtcacctt tttaccagac tcttctacaa aaaaattaag ttcgtacaat
     1861 acatataata cccgtataaa atccaactta ctcacatcgt tattgagggt aataccagat
     1921 gagctcttcg atgtttccgt atctattgca gtatccagat tagcttcaat tggcaactca
     1981 tgcagttctt ttaaatccca gtctgcactt gtaaattgat gatacatctg gcgtaatcga
     2041 gactctaagg ctgcacgatc acgttc
//
LOCUS       DAOPUR010000286         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_58497_length_2061_cov_3.024925, whole genome shotgun sequence.
ACCESSION   DAOPUR010000286 DAOPUR010000000
VERSION     DAOPUR010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58497_length_2061_cov_3.024925"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PKN90_05510"
     CDS             complement(<1..1104)
                     /locus_tag="PKN90_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNZ85770.1"
                     /translation="MKKSSFLLFFLLFSLCVFGFKSTEMRLLMQSAPVFSFFSDGSYV
                     YVSADGKSIVRKDAKTDVETLVFSIEGRSQLSSIGGFILSPDQATMLVYEKTERSSSV
                     EPLNYFLVDRQRNKLEPLSEYGKQQIPLYSPNGKMIAFVRNGDVYIKRLEFGTELRVT
                     KNGLINTLSNGLPDKLYQQGFGINSSIVWSPDSKMIAFVQYDETDVPLFTHTETTTTY
                     PQLFQQRYSKPGFPITKARLFVYTIQFRKHLEMKLPDEGDNYITAVAWSSNPELMAIN
                     VLNREQNERKTIFYNPLSGVARMANQENASPAFSPLAARFLQFLPDNSFTLLSEKTGY
                     TQLYHYANNGILIRQLTNGKMAITDVYGYDTIGK"
     gene            complement(1132..>2061)
                     /locus_tag="PKN90_05515"
     CDS             complement(1132..>2061)
                     /locus_tag="PKN90_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ85771.1"
                     /translation="FVSIFVFYVLVQSLSGFVWVRALHGLFFGLLTIANSTMAIDMMP
                     THRRGEGVGYYGATMSLAMAIGPMVSLHLYELYGNYDALFLLSFAVGMVGLAIMLFIK
                     FRCVEHIETQKEPISLDRFYLLKGTPIAFSLLTVAFSYGLIVTYVAVYGITEVGLKMG
                     ASLFFVLFAMGIMFSRFSVGKLLDKGYFSGIILVGLVLAISGLVLLVVIKNVYGFYIS
                     AIMLGVAYGIIGPAFQLLFINLAQHNQRGTANSSYFIMWDLGIGLGVLFGGNVASMAS
                     YGDSYLMAAVVLLVGLVWFRWYALSYYNQHKLR"
BASE COUNT          747 a          427 c          297 g          590 t
ORIGIN      
        1 tttccctatt gtgtcatatc catacacatc agtaatagcc atcttaccat ttgtaagttg
       61 acgtataagt ataccattgt ttgcataatg gtaaagctgt gtatatcctg ttttttcact
      121 taataaagta aaagaattat cgggtaaaaa ctgtaaaaat cgtgctgcca aaggagaaaa
      181 agctggagag gcgttttctt gatttgccat tctagctaca cctgacaatg gattataaaa
      241 aattgttttt cgttcattct gctctctatt taaaacgtta attgccatta attctggatt
      301 agacgaccat gctactgctg taatataatt atctccttca tcaggtaatt tcatctcaag
      361 atgcttacga aattgtatgg tataaacaaa taatctggct tttgttattg ggaaacctgg
      421 ttttgaatac ctttgctgaa ataactgagg atacgtagtg gtagtttccg tatgagtgaa
      481 caaaggcaca tccgtctcat cgtattgaac aaaggctatc atcttactat caggagacca
      541 tacaatggag ctgtttatcc caaaaccttg ttggtataac ttatctggca gaccatttga
      601 taaggtatta atcaatccat tttttgtaac acgcaattcg gttccaaatt ccaaacgttt
      661 gatatacaca tctccattac gtacaaaagc aatcattttt ccattaggcg aatacaacgg
      721 tatttgttgt tttccatatt cagaaagagg ttcaagctta ttgcgttgcc tatctactaa
      781 aaaataattc aatggttcaa cagaagagct acgttctgtt ttttcataca ccaacatcgt
      841 tgcttgatct ggactaagaa taaatccgcc aatagatgat aattgagaac gtccttctat
      901 tgaaaacaca agcgtttcaa catccgtttt agcatcctta cgtacaatac ttttgccatc
      961 tgcagacaca tacacataac taccatccga gaaaaaagaa aagacaggtg ccgattgcat
     1021 caataatcgc atttcggtac ttttaaaacc aaaaacacaa agtgaaaaca aaagaaaaaa
     1081 caataaaaaa ctagattttt tcatactata tctccattta actatataac attaacgcaa
     1141 tttgtgttgg ttataatatg acaatgcata ccacctaaac catactaatc caacaagtaa
     1201 aacaacagct gccattaaat aactatctcc ataacttgcc atactagcta catttcctcc
     1261 aaataataca cccaatccaa tacctaaatc ccacataata aaataagacg aattggcagt
     1321 acctcgttga ttgtgctgtg ccaaattaat aaaaagcaac tgaaacgcag gtcctatgat
     1381 gccgtatgcc actcctagca taattgctga aatataaaac ccatacacat tttttattac
     1441 taccaataaa acaagtccac ttattgctaa aacaagacct actaaaataa taccagaaaa
     1501 atagcctttg tcaagcaatt tacccaccga aaaacgtgaa aacataatac ccatagcaaa
     1561 gagaacaaag aataaacttg cccccatttt cagacccacc tcagtaatac catatacagc
     1621 tacgtatgtt acaattaatc catacgaaaa agctaccgtt agtaatgaga aagcgatagg
     1681 agtgcctttt aataagtaaa agcgatcgag cgaaattggt tctttttgag tttctatgtg
     1741 ttctacacag cggaatttaa taaaaagcat aatagctaac cctaccattc caacagcaaa
     1801 tgaaagcaaa aataaggcat cataattgcc atataattca tataaatgca aagagaccat
     1861 gggtccaata gccattgcca aactcattgt agctccataa taacccacac cttcaccacg
     1921 cctatgtgta ggcatcatat caatagccat tgtgctattg gcaatagtaa gtaagccaaa
     1981 aaacaatcca tgcaaagctc gtacccacac aaaaccgctt agcgattgta ccaatacata
     2041 gaaaacaaaa atactgacaa a
//
LOCUS       DAOPUR010000287         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_59470_length_2039_cov_3.146673, whole genome shotgun sequence.
ACCESSION   DAOPUR010000287 DAOPUR010000000
VERSION     DAOPUR010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59470_length_2039_cov_3.146673"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..1565
                     /locus_tag="PKN90_05520"
     CDS             366..1565
                     /locus_tag="PKN90_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNZ85772.1"
                     /translation="MPQTSQRGQIMPASPIRKLVPFSDKAKERGVKVYHLNIGQPDLH
                     TPEKALDAVRNIDRKILEYSPSDGLKSLRVKMLEYYQRFNIQVNLSDIIVTTGGSEAV
                     NFAFMSCLDPGDEILVPEPAYANYTAFAISAGAVVKPIVSSIEDGFALPDIARFEELI
                     TPKTKGILICNPNNPTGYLYTQEEMNQIRDLVKKHDLFLFSDEVYREFCYTGAPYISA
                     FHLEGIEENVVLFDSFSKRYSECGIRVGALVCKNKAVKESVMKFCQARLSPPLLGQIV
                     AEASIDTPKEYLEEMYNEYIERRNFLIEGLNKIPGVYSPLPMGAFYTVARLPVDDADA
                     FCSWILDEFNYEGQTVMMAPASGFYTGTTAGKNEVRLAYVLKKEELAKALVVLAKALD
                     AYPGKTI"
     gene            1594..>2039
                     /locus_tag="PKN90_05525"
     CDS             1594..>2039
                     /locus_tag="PKN90_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ85773.1"
                     /translation="MNVALFIARKLQFGNKEKKKDANPSIRIAIIGIALGLAIMLLAV
                     AIVTGFKHEIQGKVIGFGSHIQVTSLSNNTTYETPPIIFPDSLIQSIKQIDGVKHVQR
                     FATKPAIIKTDDEFQGIVIKGIDNQFNWEFFSKNLIEGETIVFNDSA"
BASE COUNT          658 a          357 c          387 g          637 t
ORIGIN      
        1 tcatttttat caacaaaaaa ggtctgtcgt gagttcgaca gacctttcgg tatatgctta
       61 ttaatatgtt ggtagtacac acaataggct tgccactcac gaatgattgt atcgtaagcg
      121 ccaccaccaa tttgtattta ctactagttt catagtcatt attgaattac gttgcaaatt
      181 tgctgctttt tttttagata aacaagtttt ttgatgtttt ttttacaata gtgcttattt
      241 ttatcttcaa tataaaaggg acataacctc aatttttatc ttctacacaa atattaaaac
      301 ctacaacttt cagaaataat aattttatac taactttgca gactttatga taatataaaa
      361 acgctatgcc tcaaacgtca caacgtgggc aaataatgcc tgcttctcct attcgcaaac
      421 tagtcccttt ttctgataaa gcaaaagaaa gaggagttaa agtgtaccat ttaaatattg
      481 gtcagcccga tttgcatacc cccgaaaagg ctttagacgc ggtacgcaac attgatagaa
      541 aaatccttga atatagcccc agtgatggac taaaatcatt acgggtaaaa atgttggaat
      601 actaccaacg gtttaacatt caagtaaact tgtcggatat tattgtaacg actggaggct
      661 ctgaggcagt aaatttcgca tttatgtcct gccttgaccc aggtgatgag atattagttc
      721 cggagcctgc atacgctaac tatactgcgt ttgccattag tgcaggggct gtggttaaac
      781 caattgtatc ttcgattgaa gatgggtttg cattgcctga tattgcccgt tttgaagagc
      841 taattacgcc caaaacaaaa ggcattttaa tttgcaatcc caacaaccct acaggctatt
      901 tatacacgca agaggagatg aatcaaatta gggatttggt taagaaacat gatttgttct
      961 tattttctga cgaagtgtac cgcgaatttt gttacacagg agcaccctat atttctgctt
     1021 ttcatttaga aggtattgaa gaaaatgtgg tattattcga ttctttctca aaacgctata
     1081 gcgaatgtgg tattcgtgta ggtgcattgg tttgtaaaaa taaagcggtg aaagaaagtg
     1141 tgatgaaatt ttgtcaagca cgtttaagtc cacctttatt aggacagata gtagccgaag
     1201 cttctattga tacgccaaaa gaatacttag aggaaatgta taatgaatac atcgaacgca
     1261 gaaatttctt gattgaagga ttaaataaaa ttccaggcgt atattctccg cttccgatgg
     1321 gtgctttcta tacagtagcc aggttgccag ttgatgatgc agacgctttt tgctcatgga
     1381 ttttagatga gttcaactac gaaggtcaaa ccgtaatgat ggcacctgct tctggttttt
     1441 atacaggcac gacggctggc aaaaatgaag ttcgtttggc atatgtactg aaaaaagaag
     1501 aattagcaaa agctcttgtg gtattggcta aagcgttaga tgcatatcca ggtaaaacaa
     1561 tctaagaagg tatatgaaac gaaacaatag cgtatgaatg tagcattatt catagctcgc
     1621 aaattacaat ttggcaataa ggaaaagaaa aaagacgcaa atccatctat ccgtattgcc
     1681 attattggca ttgcattggg cttggcaatc atgctcttag ctgtagctat tgttactgga
     1741 tttaaacacg aaatacaagg caaagttatt ggatttggtt ctcatatcca agtaacgagt
     1801 ttaagcaata acaccaccta cgaaacacct ccgattatct ttcccgattc tcttatccaa
     1861 tcaattaaac aaatagatgg agtaaaacat gtgcaacgtt ttgctaccaa acctgctatt
     1921 attaaaaccg atgacgaatt ccaaggtatt gttataaaag gtattgacaa ccagtttaat
     1981 tgggagtttt tttctaaaaa cttaattgag ggagaaacaa ttgtttttaa cgacagtgc
//
LOCUS       DAOPUR010000288         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_59477_length_2039_cov_2.746472, whole genome shotgun sequence.
ACCESSION   DAOPUR010000288 DAOPUR010000000
VERSION     DAOPUR010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59477_length_2039_cov_2.746472"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..124
                     /locus_tag="PKN90_05530"
                     /pseudo
     CDS             1..124
                     /locus_tag="PKN90_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
     gene            139..603
                     /locus_tag="PKN90_05535"
     CDS             139..603
                     /locus_tag="PKN90_05535"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNZ85774.1"
                     /translation="MKKKTYLFGLFLVLLVSCICKEPTEPVVVEDDQIVGTWYIHDYT
                     ISNVSVFAIQQCDVGSWIELKADNSMEALIKCNTPPELEGTWSREGDVINIDADKLFN
                     VNKALLSRIDNQATVLLLTDEDLQFEFAAYTTLNRKLMVKVFLSRTPLETVE"
     gene            complement(616..1332)
                     /locus_tag="PKN90_05540"
     CDS             complement(616..1332)
                     /locus_tag="PKN90_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85775.1"
                     /translation="MKKIIVIYLSCFFGSLFTIHAQLVKRKVGSDAKHQLTLLGGINF
                     PSFDIPHSIGPSAGLAYSYYFIPQIGIRGMLHYHYFDGEKNIPAIYSHTMGACVQGIF
                     NFFSYQSGRPEDAGFSFIPSKAYLVAGVGGLYHQPYINGNSQHSDLSFSIPVGIGTRH
                     PIARRWDIGVEFAWNVSSSDRIDQQVVSESTNDSFGNLFLTATYKFPKHNYGPTRGKQ
                     FQNRKTKKCDKRKGCAVAFD"
     gene            1467..>2039
                     /locus_tag="PKN90_05545"
     CDS             1467..>2039
                     /locus_tag="PKN90_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HNZ85776.1"
                     /translation="MKKVLIISYYFPPSGGSGVQRWLKFVKYLPEFGYDPIVLTVDPT
                     VASYPQIDASLLHDIPACVRVERTTTREILSLYKRVSPTKEIPYGGFANENTNTFFSI
                     LSRFVRGNLFLPDPRRGWNKFAYKKACELIEQEQIDTVITTSPPHSTQLIGLKLKKRF
                     PSIQWIADLRDPWRDIFYYKDTYPTWLADKL"
BASE COUNT          635 a          356 c          414 g          634 t
ORIGIN      
        1 gtaaaagtgt tagatacacc gtctaaatgg tttggagtaa cgtatgcaga tgatggcaac
       61 ggtattaatg aaactaaatg agttgataaa taaaggtgtt tatccagtaa aattgtggag
      121 ttaatgctcc tctaaaccat gaaaaaaaag acgtatcttt ttggattatt cctcgtattg
      181 cttgtttctt gcatttgcaa agaaccaacg gagcctgtag ttgtagaaga tgatcagatt
      241 gtgggcacat ggtatataca cgattatact atttctaatg tgagtgtatt tgctattcaa
      301 caatgtgatg tgggttcgtg gattgagttg aaagcagata attccatgga ggcactcatt
      361 aaatgcaata cgccgcctga attagaagga acatggtcga gagaaggaga tgttatcaat
      421 attgatgcgg ataaattgtt taatgtaaat aaggctttat tgagtcggat tgataatcaa
      481 gcaacagttt tacttttaac agacgaagac ttacaatttg aatttgctgc ttatactacg
      541 ctgaatagaa aactgatggt aaaagtattt ttatctcgta ctccattaga aacggtagaa
      601 taaaaaatta tttttttaat cgaatgcgac agcacatcct ttgcgtttgt cgcatttttt
      661 tgttttgcgg ttctgaaatt gtttgccacg tgtaggaccg taattgtgtt ttgggaattt
      721 gtacgttgca gttaaaaata agttaccaaa cgaatcattt gttgattcac ttactacttg
      781 ttggtcgatt ctatcagatg aggaaacatt ccatgcaaat tctacgccaa tatcccacct
      841 gcgagctatt ggatgtcggg taccaatacc tacaggaata ctaaaagata ggtcgctgtg
      901 ttgcgagttt ccgtttatgt aaggttgatg atataatcct cccacgccag ccacaaggta
      961 cgctttgctg ggaataaatg aaaatccagc atcttctggt ctgcccgatt ggtacgaaaa
     1021 gaaattaaaa ataccttgta cacacgcacc catagtatgc gaatagatgg caggaatatt
     1081 tttttctcca tcaaaataat gatagtgcaa cataccccta atgccaattt gtggaataaa
     1141 ataatacgaa taagcgagtc cagccgatgg accaatgcta tgtggaatat caaaacttgg
     1201 aaaattaata ccccctaaca acgtaagttg atgtttagca tcactgccta cttttcgttt
     1261 cactagttgt gcgtggatag tgaataggct gccaaaaaaa catgaaagat aaatgacgat
     1321 gatttttttc atagataaca gtacgattag cgacacaaaa atacgttatt tatttaatat
     1381 cattaacaag caagcagatt tttttagcaa ttagatttac tatcgctcgt aaatgtgtac
     1441 atttgcacag tatatttgct aggcatatga aaaaagtgtt gattattagt tattattttc
     1501 cgccaagtgg tgggtcgggt gtgcagcgat ggctaaagtt cgtgaagtac ctgcctgaat
     1561 ttgggtatga tcctattgtc cttacagttg accccacagt agcttcttac cctcaaatag
     1621 atgcaagttt actgcacgat atacctgcct gtgtacgcgt agaacgcaca accaccagag
     1681 aaatattgtc cttgtacaag cgtgtttctc ctactaaaga aattccatac ggaggttttg
     1741 ccaacgaaaa tacgaacact tttttctcta tcctttctcg ttttgtgcga ggcaatttgt
     1801 tcttgcccga cccgcgtaga gggtggaata aatttgctta caaaaaagcc tgtgaactta
     1861 ttgagcaaga gcaaattgat acagtaatta cgaccagtcc gcctcattct acgcaattaa
     1921 ttgggctgaa gttgaagaag agatttccct ctatacaatg gatagctgat ttacgcgacc
     1981 catggcgaga tattttttat tacaaagata cgtatcctac atggttggca gacaaattg
//
LOCUS       DAOPUR010000289         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_59659_length_2035_cov_2.807071, whole genome shotgun sequence.
ACCESSION   DAOPUR010000289 DAOPUR010000000
VERSION     DAOPUR010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59659_length_2035_cov_2.807071"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="PKN90_05550"
     CDS             complement(<1..1407)
                     /locus_tag="PKN90_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNZ85777.1"
                     /translation="MIYPAHFETKIGFDSIRAILKHHCISPLGEEKVDEMTFSDSLAT
                     IDTRLSQTNELLTLLNEHDSFPTDAYVDIRPALSRIRIEGTYLLENELFDLYRSLLTI
                     QHIVQFIEQKDAKRFEQLHLLAKDVLLFPPITKHIETILSKFGKIKDSASPELASIRR
                     SKSQLSGTISRLLQQIIRKGQTDGYIDNEVTPAVREGRLVIPVSPSYKRKINGIIHDE
                     SASGKTIFIEPAEVVETNNQIRELELQEKREITRILVQCANFIRPMLSDLLHSYHFLG
                     TIDFIRSKALLAIQLQATKPVIENNPHIDWQDTFHPLLYLSLRKQGKTIVPLSIKLND
                     QERILVISGPNAGGKSVCLKTVGLIQYMMQCGLLVPMKESSTMGIFQRIFIDIGDEQS
                     IENDLSTYSSHLTNMKFFAKHCQEATLLLIDEFGTGTEPLIGGAIAEATLHVFNQQKA
                     FGVITTHYTNLKHFATENE"
     gene            1554..1638
                     /locus_tag="PKN90_05555"
     tRNA            1554..1638
                     /locus_tag="PKN90_05555"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1588..1590,aa:Ser,seq:tga)
     gene            1770..1970
                     /locus_tag="PKN90_05560"
     CDS             1770..1970
                     /locus_tag="PKN90_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85778.1"
                     /translation="METRITPHTPRLEQQHYAHPKHGRQKSLVYFESSLKRLTFEQKL
                     NYTEVFLDRLMLAVILGAKQTH"
BASE COUNT          617 a          354 c          423 g          641 t
ORIGIN      
        1 ttcgttttcg gtggcaaagt gttttaaatt cgtataatgg gtagtaatga caccaaaagc
       61 tttttgttgg ttaaatacgt gtaaggtagc ctccgctatt gcacctccaa taagcggttc
      121 agtacctgtg ccaaattcgt cgatgagtag cagagtagct tcttggcagt gtttggcaaa
      181 aaacttcata ttcgttaaat gcgaactgta tgtacttaaa tcgttttcaa tactttgttc
      241 atcgccaata tcgataaaaa ttcgctgaaa gatacccata gtgcttgatt ctttcatcgg
      301 cactagtaag ccacattgca tcatatactg aataagacct actgttttta aacataccga
      361 tttgccgcct gcattcggtc cagagatgac caaaatacgt tcttggtcgt ttagtttgat
      421 acttagtgga acaatcgttt tcccttgttt gcgaagtgat aagtaaagca agggatgaaa
      481 cgtatcctgc caatcaatgt gcgggttgtt ttcgataaca ggttttgtcg cttgtagctg
      541 aatggctagc agtgctttgg agcgaataaa atcaatggtg cctaaaaaat gatacgaatg
      601 aagtaaatcg gataacattg gacgaataaa attggcacat tgcaccaaga tgcgggtaat
      661 ttcgcgtttt tcttgtagct ccaattcgcg aatttgatta tttgtttcca cgacttcggc
      721 aggttcaata aaaatggttt ttccactggc agattcgtcg tgtataatcc cattaatttt
      781 gcgtttgtac gaaggagaaa cgggaattac tagtcgtcct tcgcgaacag caggcgttac
      841 ttcgttgtca atgtatccat ctgtttgccc tttgcgaata atttgttgta ataaacgtga
      901 gatagtgccc gaaagttgcg atttagaacg cctaatagag gctaattcgg gcgaagcact
      961 gtccttaatt ttcccaaatt ttgataaaat cgtttcaatg tgttttgtaa taggagggaa
     1021 taataacaca tcttttgcta agaggtgtaa ttgttcaaaa cgtttggcat ctttttgttc
     1081 gataaattgt acaatatgtt gtatggtaag cagcgatcgg tataaatcga acaattcgtt
     1141 ttcgagcaaa tacgtacctt ctatacgaat acgcgataag gctggacgaa tatccacata
     1201 cgcatctgtt ggaaacgaat cgtgttcgtt gagtaaggtt aacagctcgt ttgtttggct
     1261 taagcgtgta tcaatagtag caagcgaatc cgaaaatgtc atttcatcca ctttttcttc
     1321 gccaagcgga ctaatgcaat ggtgttttag aatggctcga atgctatcaa atccaatttt
     1381 tgtctcaaaa tgagcaggat aaatcataca tgtgcttgct ttattacgat acaaaggtag
     1441 tttatttgtt gtaatggagc aatagctgta gttttttata aaaatcaata taaaaatttg
     1501 caagagaaaa aaaaagcaat tacatttgca caacttttgc taaagcaaga taaggagagg
     1561 tggcagagtg gtcgattgcg gcggtcttga aaaccgttgt actgcgaggt accgggggtt
     1621 cgaatccctc cctctccgcg aaaaatagct gattatcagt gcgttgcaaa attatcaccc
     1681 attttagccc ttataaaggt tgaaatgggt gattttgtta tattgatagg tggcttgtcc
     1741 tgacataatt ttacccaaaa aggtgtaaaa tggaaacaag gattactccc catacccctc
     1801 gtttggaaca acaacactac gctcacccca agcatggtag gcaaaaatct ctagtatatt
     1861 ttgaaagttc gttaaaacgt cttacatttg agcaaaaatt aaactacacg gaggtttttt
     1921 tagacagact gatgttagca gttattttag gagcgaaaca gacacattag agaaatcaca
     1981 ataaaatgaa aagattatct gtagtactga atttgattat aattagtagt attat
//
LOCUS       DAOPUR010000290         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_59967_length_2028_cov_3.501267, whole genome shotgun sequence.
ACCESSION   DAOPUR010000290 DAOPUR010000000
VERSION     DAOPUR010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59967_length_2028_cov_3.501267"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PKN90_05565"
     CDS             <1..675
                     /locus_tag="PKN90_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ85779.1"
                     /translation="TIPKGKVTAIIGESGSGKTTLAMILQKLYPINSGKITINNLNIN
                     HFTYESLRNLISTVPQQLNLFSGNIIENIAIGDLQPDMERIVAIVQQLGLMPFIEKLP
                     TGFHTQIGENGTSLSGGEKQRLAIARALYRNPEILIFDEATSSLDSESEQYVKNTIQS
                     LKQQGKTILIIAHRLSSVIDADKIAVLGNGELKEEGTHHALYVEGTQYYKMWQKQIPH
                     FIIENK"
     gene            844..1749
                     /locus_tag="PKN90_05570"
     CDS             844..1749
                     /locus_tag="PKN90_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ85780.1"
                     /translation="MSEKSGYLLVNMLDMIKSYLAILVFIFVSMKLVAEQEHKMPPSN
                     TIGICLSGGGALGFSHIGVLQALEDNGIKPAYISGSSMGAIIGVLYAQGISPKEMMKI
                     IPKEKMNRFSRIWKLPSSILGFSSMITVHELLSKYVPHNRFDHLQIPLFVCVTNLYSG
                     EWEIKSTGDRLIDYVCASAAIPSVFEAQIIDSMHYVDGGVLNNLPVEPLLDKCDVIIG
                     VDVLEYPRKKELKKASDVVLYSILAANAETHRVRKPYCNYYISCNGASTYSIFDFHAF
                     KELYEIGYQRTVEYIQAHPEMLLHR"
BASE COUNT          671 a          335 c          395 g          627 t
ORIGIN      
        1 actattccaa aagggaaagt aacggcaata attggagaga gtggttctgg aaaaacaaca
       61 ttagcaatga tattacaaaa gctatatcca attaattcag gtaaaataac tattaataac
      121 ctaaacatta atcattttac ttatgagagt ttgcgaaatc ttataagtac cgtaccacaa
      181 caactcaatt tattttcagg aaacatcata gaaaacattg ccattggcga ccttcagcct
      241 gatatggaac gaattgttgc tattgtgcaa caacttggac taatgccctt tatcgaaaaa
      301 ctaccaacag gattccatac ccagattggc gaaaatggca catcgctttc agggggcgaa
      361 aaacaacgct tggccattgc acgtgcatta tatcgcaacc ccgaaatatt gatattcgac
      421 gaagcaactt cgtcgttaga ctccgaatcg gaacaatatg taaaaaatac cattcaatcg
      481 ttaaaacaac aaggtaaaac tattctcatt atcgcacaca gacttagtag cgtaattgat
      541 gctgacaaaa tagctgtttt ggggaatgga gaactaaaag aggaaggtac tcatcatgca
      601 ttatatgtag aaggcacaca atattacaag atgtggcaaa aacaaattcc tcattttata
      661 atagaaaata aataattacc taataatctt tagcatatct aacatatact taattaagta
      721 tcatcataac atatagaatg actttcgatt tttacttata agacgtttta gaagtagttt
      781 ttatgcttct tgctgtatat attttgtgtt tgttgagaac aaaagtaaca aaataattgc
      841 tttgtgtctg aaaaaagcgg atatttgttg gtaaatatgc ttgatatgat aaaaagctac
      901 cttgcaattt tagtattcat ttttgtatca atgaaattag tagcagaaca agaacataaa
      961 atgcccccat cgaatactat tggtatttgt ttaagcggag gaggagcttt agggttttcg
     1021 catatcggtg tgttgcaagc cctcgaagac aatggtataa aacccgccta tatttcgggg
     1081 tcgagtatgg gtgccataat tggggtattg tatgctcaag gcatttcgcc caaagagatg
     1141 atgaaaatta ttccgaaaga gaaaatgaat cgcttttcgc gtatttggaa attaccaagt
     1201 tctattttgg gtttttcaag tatgattacg gtacacgaac tattgtcaaa atatgttccc
     1261 cataatcgtt ttgaccactt gcaaataccc ttgtttgtgt gtgtaactaa cctgtattca
     1321 ggcgaatggg agattaaatc aacgggagat agattgattg attatgtgtg tgcatcggct
     1381 gctataccct ctgtgtttga ggctcagatt atcgactcca tgcactatgt agatggagga
     1441 gtattaaata atttgcccgt tgagccattg ttggataagt gcgatgtgat tattggtgta
     1501 gatgtgttgg aatatcccag aaaaaaggaa ttaaaaaaag cctctgatgt cgttttatat
     1561 tctattttag ccgccaatgc cgaaacccat cgggtacgaa aaccctactg caattactat
     1621 atatcgtgca atggagcttc tacctattcg atttttgatt ttcatgcctt caaggagttg
     1681 tatgagattg gctaccagcg tactgtagag tatatacagg ctcatccaga gatgttgttg
     1741 catagatgaa gactcgttga taattgataa ttgataattg atagttgata gttgatagtt
     1801 ggagctgttc aaagttcatt acccctcttt ggtgcaagtt tagcgatagc gtaacttgta
     1861 cctataataa aagcagtttg taactgcgta aaaaaaggaa attaaaagaa taaatgagta
     1921 ggaaatatat atcccagcta gcgcggattt gtaatccgtg ctttaactat tgatcccctc
     1981 tttggtgcaa gtttagcgat agcgtaactt gtacctataa taaaagca
//
LOCUS       DAOPUR010000291         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_60153_length_2024_cov_3.125444, whole genome shotgun sequence.
ACCESSION   DAOPUR010000291 DAOPUR010000000
VERSION     DAOPUR010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60153_length_2024_cov_3.125444"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKN90_05575"
     CDS             complement(<1..237)
                     /locus_tag="PKN90_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85781.1"
                     /translation="MNSIVNIGVDAGSTTVKVVVTDEKHTILFKEYLRHLADVAGVIS
                     TVLGSLLHTLGDKSVHITITGSAGMGIAERCSLPF"
     gene            complement(259..1371)
                     /locus_tag="PKN90_05580"
     CDS             complement(259..1371)
                     /locus_tag="PKN90_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ85782.1"
                     /translation="MIKYLLKKEFKQIIRNPFLPRMILFFPFVVLLVFPWAANYEIRN
                     MNASIVDNDHSSYSRRLTQTIVSSEYFKLADFSSTYNEALKSIEQNKADVIIQIPANF
                     EKDIINRNGTKIMISPNAVNGMKGGIAVAYLSQIVSKFNSTILVERFPQSAVMSNSFI
                     QTDSLFQFNKELNYQVFMVPALMVMILTLLCEFLPAVSIVFEKEIGTMEQINVSPVPK
                     IVFILAKLIPFWLIGFIVITIGFGVAWGVYGLVPNGHFYTIYIFAFIFILGISGLGLV
                     ISNYAQTMQQAVFIVFFFMMIFIMMSGLYTPIDSMPNWGQWIAAFDPLKYFMSVMRSV
                     YLKGSDTIHLLKDLCILCMFAVFFNAWAIISYRKSQ"
     gene            complement(1410..>2024)
                     /locus_tag="PKN90_05585"
     CDS             complement(1410..>2024)
                     /locus_tag="PKN90_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ85783.1"
                     /translation="NPQMKGAYNFVPGVLGMIMILICAMMTSIAIVREKEMGTMEILL
                     VSPMKPIYIVLSKIAPYFVLSCINFATIILLSLFVLNVPVAGSFASLIVVSLIYIFVS
                     LALGLLISTLTSSQLAAMMISGMALMIPVMLLSGMIFPVENMPTWLQWISNVVPARWY
                     ISAAKSIMIKGLGITSVLKELSVLSVMGITLLLISLKKFKIRLE"
BASE COUNT          776 a          389 c          302 g          557 t
ORIGIN      
        1 aaaaggcaaa ctacaacgct cagcgatacc cataccagcc gaaccggtaa tggttatgtg
       61 aaccgacttg tctcctaagg tatgcaataa agaccctaag acagtagaaa taacccctgc
      121 tacatcggct aaatgacgga gatactcttt gaacaaaata gtatgctttt catcagttac
      181 taccactttt actgtagttg aaccagcatc tacacctata ttgactattg aattcattat
      241 tcagcttata aatattcatt attgactttt acggtaacta attatcgccc aagcattaaa
      301 aaacacagca aacatacaca atatacacaa atcttttagt aagtgaatcg tatcactccc
      361 ttttaaatag acagacctca ttaccgacat gaaatatttt aaaggatcaa atgcagcaat
      421 ccattgtccc caattaggca tgctatctat cggtgtatat agaccactca tcataataaa
      481 aatcatcata aaaaagaaca caataaaaac ggcttgttgc atagtttgtg cataattaga
      541 aataactaga ccaagaccag atattcctaa aataaagatg aacgcaaaga tataaatggt
      601 ataaaaatgt ccattaggta ccaatccata aacaccccaa gcaacaccaa aacctatggt
      661 gataacgata aacccaatta gccaaaaagg gattaattta gccaaaataa atacaatttt
      721 aggcacagga cttacgttaa tttgttccat agttccaatt tctttttcaa aaacaatgga
      781 aacggcaggt aaaaactcgc ataaaagagt taaaatcatg accataagag caggtaccat
      841 aaacacctga tagtttaatt ctttattgaa ctgaaacaga ctgtctgttt gaataaaaga
      901 attgctcatc acagccgatt ggggaaatct ctccacgaga atagtcgaat taaatttgct
      961 tacaatttgt gaaagataag ctacggcaat acctcctttc attccattga cagcattcgg
     1021 actaatcatt atttttgttc catttcgatt aatgatgtct ttctcaaaat ttgcaggaat
     1081 ctgaataatt acatctgctt tattttgctc tatacttttc aacgcttcgt tgtaagttga
     1141 agaaaaatca gccaatttaa aatactcaga agaaacaatg gtttgagtaa gacgtcgcga
     1201 ataagatgaa tgatcattat caacaatact tgcattcatg ttgcgtattt cgtaattggc
     1261 agcccaagga aaaacaagca acaccacaaa tggaaaaaaa agtatcattc tcggaagaaa
     1321 aggattccga ataatttgct taaattcttt tttgagaagg tattttatca tacaacatac
     1381 attaataaaa aaactattcg ttacccaaat tattcgagtc gtatcttaaa ttttttcaaa
     1441 cttattagca ataaggtaat tcccataaca gaaagaactg aaagctcttt caaaacggat
     1501 gtaattccta agcctttaat cataatagat ttagcagcag atatatacca acgtgctggc
     1561 acaacgtttg aaatccattg taaccacgtt ggcatgtttt caacggggaa aatcataccg
     1621 gaaagtagca taacaggaat cattaacgcc atacccgaaa tcatcatagc ggctagttgc
     1681 gaacttgtaa gagtagatat taataacccc aatgccagtg aaacaaatat ataaatcaac
     1741 gaaacaacta tcaaactagc aaaactacca gcaacaggaa cgttaagaac aaaaagagaa
     1801 agtaatataa tcgttgcaaa attgatacac gaaagaacaa aataaggtgc aattttagac
     1861 aatacaatat aaatcggttt catcggcgaa acgagcaaaa tttccattgt tcccatttcc
     1921 ttttcacgga caatggcaat ggaggtcatc atagcacaaa ttaaaatcat aatcatgccc
     1981 aaaacaccag gaacaaaatt ataagctccc ttcatttgtg gatt
//
LOCUS       DAOPUR010000292         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_60191_length_2023_cov_3.762195, whole genome shotgun sequence.
ACCESSION   DAOPUR010000292 DAOPUR010000000
VERSION     DAOPUR010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60191_length_2023_cov_3.762195"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2023)
                     /locus_tag="PKN90_05590"
     CDS             complement(<1..>2023)
                     /locus_tag="PKN90_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ85784.1"
                     /translation="KLDEGGFSPTTNLLFVYVKADLLLKQKRYQEAVPFLESAYNAEK
                     KQQQQARLGFILGQIYEKEQQYDKAFTYYNYVLKKNPPYEMAFNANLKKTQSYQGKDY
                     EKMIKDVTKLASNERNYEYLDQLYTAIAKLYQRNGKTTKAIEYYQLAIEKSTRNGMDK
                     AEALLSLGQLYINNKEYVKAQPLINEAVPILPSSYSNYRSWRTISENLNELALHHNRI
                     VLEDSLQKLAQLPEIERIKRIQEVIKVEQEAQEKLKEKIDKEAKQQEERERNAFSTLP
                     SLALGEIGDKSWYFYNPTLIGRGKIEFQRTWGNRILEDNWRRKNKTAVLNDRQETIDE
                     VVQKIEPSSTTENVTQQPDSISPTDKQIAKYLQQIPLSVAQKENSDNAIMESLYNLVY
                     IYKEKVPDYELAQQTYAELIERFPSSSFIAEATFNMFQLHSKEQNLLEAERSKQYILA
                     NFPTSTYAVFLQNPEELNTLTQLRKEEEKLYAETYEAFLKNDYAIVALNLEKAQKEFP
                     FSKLTPKFRFLEALSTSKKKGKDAFKTSLELIVKDYPDSEITPMAKDMIALMKQGQEP
                     QSSLSHSNLLAERTSVVTEQVFAENIAKAGFVYEPQDNHLFVLIVNTDSITRNTLIFN
                     IASYNFTRFLIKELDIQVKELDDVNVAIAISGLDNLSEAIWYQKTLLADS"
BASE COUNT          506 a          337 c          372 g          808 t
ORIGIN      
        1 actatctgct aacagtgttt tttggtacca tatagcttca gataaattat ctaatccact
       61 aatggcaatt gcaacattta catcgtctaa ttcttttact tgaatatcca attctttaat
      121 taaaaaacga gtaaagttat acgatgctat attaaaaata agagtatttc gggtaattga
      181 atctgtgtta actattaata caaataaatg gttgtcctgt ggttcgtata caaaacctgc
      241 tttggcaatg ttttctgcaa atacctgttc cgttactacg ctagtgcgtt cggcgagcaa
      301 gttgctgtgc gaaagagagg attggggctc ttgtccttgt ttcattaggg ctatcatatc
      361 ttttgccatt ggtgtaattt cactgtcagg atagtccttt acgataagtt caaggcttgt
      421 tttaaaagca tctttccctt tctttttact agtgcttaat gcctctaaaa agcgaaactt
      481 tggcgtaagt ttagaaaaag gaaattcttt ttgtgctttt tccaagttta aggcaactat
      541 agcataatca ttcttcagaa atgcttcgta tgtttcagca tagagttttt cttcttcttt
      601 tcgtaattgt gttagggtgt tgagctcctc tggattttgc aaaaaaacag cataggttga
      661 agttgggaaa ttcgctaaaa tatattgttt gcttctttct gcctctaaca agttttgttc
      721 tttactatgt aattgaaaca tattaaatgt tgcttcggca atgaaagagg acgaaggaaa
      781 tcgttcgatt aattcagcat atgtttgttg agccaattca taatcaggta ctttttcttt
      841 gtatatgtac acaagattgt ataaactttc cataatggca ttatcagaat tttctttttg
      901 tgccactgaa agcggtatct gttgtaaata cttcgctatt tgtttgtctg taggtgatat
      961 ggagtctggt tgttgtgtta cattttctgt tgttgacgag ggttctatct tttgaacaac
     1021 ttcatctatt gtttcttgcc tatcatttaa tactgctgtt ttgttttttc ttcgccagtt
     1081 atcctctagt atcctatttc cccaggtacg ttgaaattct atttttcctc ttccgattaa
     1141 tgtggggttg taaaaatacc atgatttatc tccaatttcc cctaaagcga gggagggaag
     1201 agtagaaaat gcattgcgtt cgcgttcttc ttgttgtttt gcctctttgt caatcttttc
     1261 ttttaatttt tcttgagctt cttgttctac ttttattact tcttgtatcc gttttatgcg
     1321 ttcaatttca ggtaattgtg ccaatttttg gaggctgtct tctagcacta ttcgattgtg
     1381 gtgtaaagca agttcgttca gattttcgga tattgtacgc cacgaacgat agttagaata
     1441 tgaggaaggc aagataggta cagcctcatt tatcaaaggt tgtgctttta cgtattcttt
     1501 gttgttgatg tatagctgtc caagagacaa aagagcttct gctttatcca tgccattgcg
     1561 tgtgcttttt tctattgcaa gttggtagta ttctattgcc ttagtcgttt ttccgtttcg
     1621 ttgataaagt tttgctattg ccgtatatag ctgatctaag tattcataat tacgttcatt
     1681 ggatgctaat ttagttacat ctttaatcat tttttcgtaa tcttttcctt ggtacgattg
     1741 agttttcttc aagttggcat taaacgccat ttcatacgga ggattttttt tcagtacgta
     1801 gttgtaatac gtaaatgctt tgtcatattg ctgttctttt tcgtaaattt gtcccaagat
     1861 aaatcctaag cgagcttgtt gctgttgttt tttttcagca ttataagctg attctaaaaa
     1921 agggacggct tcttggtatc gtttttgttt gagtagtaaa tccgctttta catacacaaa
     1981 caataaattt gttgtcggac taaaccctcc ttcgtcaagc tta
//
LOCUS       DAOPUR010000293         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_60348_length_2020_cov_3.234606, whole genome shotgun sequence.
ACCESSION   DAOPUR010000293 DAOPUR010000000
VERSION     DAOPUR010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60348_length_2020_cov_3.234606"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKN90_05595"
     CDS             complement(<1..652)
                     /locus_tag="PKN90_05595"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNZ85785.1"
                     /translation="MTFLPYNQDVDATVKEIMIKIRVAMNGVTSELMSDRGVKYKTNF
                     GVAFPDLQKIAQQYSPNELVATYLWHKSVRETKILAILLYPAQALTLEKSITWISDCD
                     TVELIEYLCAHLVVFTPFVPTLITQLARISEEKQIIAAYTLAAHYCKRNKPELTEEFS
                     PLLSFSSIHITAKLAQSMLYFLLYAYQKNEFKENIINEMQNLKKNQSENHLAMWLYT"
     gene            781..856
                     /locus_tag="PKN90_05600"
     tRNA            781..856
                     /locus_tag="PKN90_05600"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:His,seq:gtg)
     gene            1150..>2020
                     /locus_tag="PKN90_05605"
     CDS             1150..>2020
                     /locus_tag="PKN90_05605"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ85786.1"
                     /translation="MLQRYLDTYWVNLLLLKSVVISVLFISSCSTEEPTPKPSTLCNI
                     SEISFESEQSAMLITVNTKESWSIEHLSNWIDFTATTGRGKTSLLVGATKNDGFGRID
                     SFLIKTAKQTHKIYIEQKGAAIVHLDILSHTLVFVKVEGGTFKIGNYQNPHEHDVSLT
                     EYYIGQTEVTNAFWEAIVGSLPYSNENKNYPYTMDDDNPDFPVTAVSWNDIHTTFLPL
                     LHTKTSHTFRLPTEAEWEFAALGGNKSKGYTFSGSNILDVVGWDASNANDTKQAVAQL
                     EPNEINLYDMSGNA"
BASE COUNT          677 a          344 c          353 g          646 t
ORIGIN      
        1 cggtatacag ccacattgcc agatggtttt ctgattgatt ctttttcaga ttctgcattt
       61 cattgataat gttctcctta aactcatttt tctggtaagc atacaataaa aaatacagca
      121 ttgattgagc caatttggct gttatatgga tggaagaaaa tgataaaagt ggagaaaatt
      181 cttctgtcag ttctggttta ttacgtttac aatagtgagc agccaaggta tacgcagcaa
      241 ttatttgttt ttcttctgaa atacgggcaa gttgtgttat caaagtggga acaaaaggag
      301 taaataccac taaatgagca cataagtact ctattaattc cactgtatcg caatcagata
      361 tccacgttat agatttttcg agagtgagtg cttgtgcagg atacaaaaga atggctaaaa
      421 tttttgtctc ccttaccgac ttatgccata gatacgtagc tactaactca ttaggagaat
      481 attgttgggc aatcttctgc aaatcgggaa aagcaacgcc aaaattcgtt ttatacttta
      541 cgcccctatc cgacattaat tcggaagtaa cgccattcat tgctactcga atttttatca
      601 ttatctcttt tacagtagca tcaacatctt ggttatatgg taaaaatgtc ataattaagt
      661 tatttttaaa tgacaaagat agtatttagc tcgtagctta aaaaaaatac agctaaaaaa
      721 cgcttaatac tattgcataa aatcaaactt agtagtatct ttgcaacgtt tttaacacac
      781 ggtgattgta gctcagttgg ttagagcgtc ggattgtggt tccgaaggtc acgggttcga
      841 gccccgcctt tcacccttaa aaaagcaagt aacaaataac acacgattat tgttgcttgc
      901 ttttttgatt tatgagctac ttcaggctat attattctta cttctacaac tattttcata
      961 catactttta gaaaatagaa tttacacaga gggtattaaa cgtattttag ttaacaaaca
     1021 ttgaagattg ttaataaata tctacttttt attattaatt attcaatcaa aatttgtagc
     1081 tttgtgatac tatagttgta ttggactatt atatagcttg caccataaca agtataattg
     1141 tttttagata tgttacaacg atacttagat acatattggg ttaatttact attgctgaaa
     1201 agcgtagtga taagtgtatt attcatatct tcttgttcta cagaagaacc cacccccaag
     1261 ccttctactt tgtgcaatat ctctgaaatt tcgtttgaat cagagcaatc agctatgctc
     1321 attacagtaa atacgaaaga aagttggtcg atagaacatc tgagcaattg gatagatttt
     1381 actgcaacaa caggacgtgg caaaacttct cttttagtag gtgcaaccaa aaatgatggg
     1441 tttggtcgca tcgactcatt cttaattaaa acggcaaaac aaacgcacaa aatatacatt
     1501 gaacaaaaag gagcagccat tgtgcatctt gatattttat cccatacact cgtttttgtg
     1561 aaagttgaag gcggaacatt taagataggg aattatcaaa atccacacga acatgatgta
     1621 agtctaactg agtattatat tggtcagaca gaggtaacaa acgctttttg ggaggctatt
     1681 gttggctcac taccatatag caacgaaaat aaaaattacc cctatacaat ggatgatgat
     1741 aatccagatt ttcctgttac agcagtttca tggaacgata ttcacactac atttctaccc
     1801 ctattacata ctaaaacttc gcatacattt aggctaccta cagaagctga atgggaattt
     1861 gcagcattgg gaggaaataa aagcaaagga tatacatttt cgggtagtaa tatacttgat
     1921 gtagtaggtt gggatgcatc aaatgctaac gatacaaaac aagcagtagc acaattagaa
     1981 cccaatgaaa taaacctata tgatatgagt ggcaatgccg
//
LOCUS       DAOPUR010000294         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_60874_length_2009_cov_3.037359, whole genome shotgun sequence.
ACCESSION   DAOPUR010000294 DAOPUR010000000
VERSION     DAOPUR010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60874_length_2009_cov_3.037359"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKN90_05610"
     CDS             complement(<1..206)
                     /locus_tag="PKN90_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85787.1"
                     /translation="MSESDWTEYVTFFTATEDADNISLSLTNERVRNNTEGNDFVIDD
                     ILFQAVSAEESNLFEVFKIRVLPLP"
     gene            complement(224..373)
                     /locus_tag="PKN90_05615"
                     /pseudo
     CDS             complement(224..373)
                     /locus_tag="PKN90_05615"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
     gene            complement(521..736)
                     /locus_tag="PKN90_05620"
     CDS             complement(521..736)
                     /locus_tag="PKN90_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85788.1"
                     /translation="MSTHDNLKSLAEAYFEGKISLEEERQLFAFINDNDDKKKQFYEY
                     EENWKKHYASEDTIKAWQKVYNRMQVA"
     gene            complement(836..1402)
                     /locus_tag="PKN90_05625"
     CDS             complement(836..1402)
                     /locus_tag="PKN90_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNZ85789.1"
                     /translation="MNFSDPHILLSEIQKGNHLAFEFLFKAYYPRLCNFAARFVDSTT
                     AEDIVQECFLKFWEKRFSIKQGNILSLLFTMVRNACLNHLKHKALVDISSIDFVKNLS
                     GEEALYALDFTGGADYVCLEHELAEQINKVMNDLPARCREVFVLSRMDGLKNKEIALQ
                     LNISTTMVEKHIAKAIAVFSAKFKDDIG"
     gene            1792..>2009
                     /locus_tag="PKN90_05630"
     CDS             1792..>2009
                     /locus_tag="PKN90_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85790.1"
                     /translation="MIEQVYKNLSSKLETVQQTLQRPLTLTEKIYILDTNILINFSIW
                     CPMEFNEGFWLKLEKLLEEGKWILLDVVV"
BASE COUNT          689 a          371 c          266 g          683 t
ORIGIN      
        1 ggtaaaggaa gcacccttat cttaaatacc tcaaataagt tactttcttc agccgaaaca
       61 gcttgaaata agatatcatc tatcacaaaa tcattccctt ctgtattatt ccgtacgcgt
      121 tcatttgtaa gcgaaagtga tatattgtct gcatcttctg tggcagtaaa aaaagtaacg
      181 tattctgtcc aatctgattc tgacaaaata tttgtttccc tttgtgcttt gtaacctcaa
      241 agtaggcttc gccatctaaa taaactaatc ggttattgct aaaatcatcg tcatacgtta
      301 aggttgatcc agcatttaac cacacttttg ttccatcaga gagaataatt gttccttgac
      361 ctccgtaagc caccctaaat tcatatcgtt ctttttctcc ccaagtacca gaatgcaaga
      421 aaaatagtcc cgaaaagcca attaacaata ttagtactgc cgcagcagac aatagtttcc
      481 aagaaaaacg agactgagat actgaaggaa gaacgactgt ttatgcaact tgcatacgat
      541 tatatacttt ttgccaagcc tttattgtat cttcactagc gtagtgtttt ttccaattct
      601 cctcatattc gtaaaattgc tttttcttat catcgttgtc attgatgaaa gcaaacaatt
      661 ggcgttcttc ttctaaactt atttttcctt caaaatacgc ttcagctaaa ctcttgaggt
      721 tgtcatgtgt actcataatt atactatctt gtatatatat agcaatcaat gtcgtatttt
      781 atccaaccta aacaacgtta aataacgtta aaacggcact agtatctgcc attagctagc
      841 caatatcatc tttaaacttt gcagaaaaaa cggctattgc cttggcaata tgtttttcaa
      901 ccattgttgt agaaatattt aattgaagag ctatctcttt attttttaat ccatccattc
      961 tgcttaaaac aaacacctct ctacaccgag caggtaaatc attcattacc ttattaatct
     1021 gctctgctag ttcgtgttcc aaacaaacat aatctgctcc tcccgtaaaa tccaatgcat
     1081 acaaagcctc ctctcccgac aaatttttca caaaatctat tgaagatata tcaactagag
     1141 ctttatgttt taagtgattt aggcatgcat tgcgaaccat cgtaaataat aaagagagga
     1201 tattgccttg ttttatagaa aatctctttt cccaaaattt cagaaagcac tcttgaacaa
     1261 tatcttctgc agtagtagaa tctacaaatc gagctgcaaa attacacaaa cgaggataat
     1321 atgccttgaa taaaaattca aaagccaaat gattgccttt ttgaatttcg gacaacaata
     1381 tgtgtggatc tgaaaaattc atagtgtgat ttagatttta caaaggtagc aattttacaa
     1441 aataaaatat tctttcaagc tatttttttc gcataacaat gtatctcaac gaaaactatg
     1501 ttctcctata ctcattctat tcatattttt ataccataat attgattata aaattgagct
     1561 ctcattaaac aataattaat cgaaacaagt atctattatt tgttatcaca ggactctcat
     1621 tttgtatata ttccgcatat atacaaaata aaacactaaa aactctaaat tgctacattt
     1681 tttcatacaa aaaagcactg ctttctattt tctcatagtt tttttagtat atttgcaaaa
     1741 acgaaaaatt taactacaaa ctgaaagaat aatatggttt ttgatgagaa aatgattgaa
     1801 caagtatata aaaacttgtc atcaaagctt gaaacagtac aacaaacact acaacgtcct
     1861 ctcaccttaa cagaaaaaat ttatatactt gatactaata ttttgattaa tttttcaatt
     1921 tggtgcccca tggaatttaa tgagggattt tggttaaaat tagaaaaatt attagaagaa
     1981 ggaaagtgga ttttattaga tgttgttgt
//
LOCUS       DAOPUR010000295         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_60879_length_2009_cov_2.810133, whole genome shotgun sequence.
ACCESSION   DAOPUR010000295 DAOPUR010000000
VERSION     DAOPUR010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60879_length_2009_cov_2.810133"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..398)
                     /locus_tag="PKN90_05635"
     CDS             complement(45..398)
                     /locus_tag="PKN90_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNZ85791.1"
                     /translation="MKVVRILRNIVLGLFLSSILLVILYRFVPVYITPLMIIRVAEQV
                     ADGKPIRLERGWVPIDEISPKMVRAVIASEDNLFLDHYGFDIEGMKKAFENNKKGKKL
                     KGGSTIHNKQPKMFF"
     gene            complement(416..1453)
                     /gene="asnA"
                     /locus_tag="PKN90_05640"
     CDS             complement(416..1453)
                     /gene="asnA"
                     /locus_tag="PKN90_05640"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNZ85792.1"
                     /translation="MGKLIVPQTYTSLLDLQQTELAIKILKDFFQTSFSAELRLRRVT
                     APLFVLQGMGINDDLNGVERAVSFPVKDLGDVKAEVVHSLAKWKRIILADYAIENGYG
                     VYTDMNAIRSDEELDNLHSLYVDQWDWEMVIAKEDRTIAFLQQVVKRIYGVMVRAEYA
                     VYEYFPTIKPSLPRDIFFIHAEELLAMYPDKSPKEREYMVTKEHGAVFIMGIGGQLAN
                     GEKHDGRAPDYDDWTTIGENGLAGLNGDILVWDSVLQMPLELSSMGIRVDKDALIKQL
                     AITHTEERLQFYYHKRLVEGSLPLSIGGGIGQSRLCMFMLRKAHVGEIQASIWPDSMR
                     KECKELGIFLL"
     gene            1675..1746
                     /locus_tag="PKN90_05645"
     tRNA            1675..1746
                     /locus_tag="PKN90_05645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1708..1710,aa:Arg,seq:ccg)
     gene            1940..>2009
                     /locus_tag="PKN90_05650"
     CDS             1940..>2009
                     /locus_tag="PKN90_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HNZ85793.1"
                     /translation="MLGHSSSKTTEIYTHITNKGFDQ"
BASE COUNT          678 a          399 c          309 g          623 t
ORIGIN      
        1 aaaataagct tccaaagctt tacgaacata cgtacgactc ggggttaaaa aaacattttt
       61 ggctgtttgt tgtgaatagt gctcccccct tttaattttt tccccttctt gttattttca
      121 aaggcttttt tcattccttc gatatcaaaa ccgtaatggt ctaaaaaaag attatcttct
      181 gaggcaataa cagcacgtac cattttgggt gaaatttcgt ctattggcac ccagcctctt
      241 tctaatcgaa tgggtttacc atcagccact tgttctgcca ccctaataat cattaaagga
      301 gtaatataaa caggtacaaa acgatacaat ataacaagca agatactgga taaaaataat
      361 ccaagtacga tgttacgaag aatacgcact actttcattc aaattggtaa atttcttata
      421 gcaaaaatat acctaactct ttacattctt tacgcatact atcgggccaa atacttgctt
      481 gtatctcacc tacgtgagcc ttacgtaaca taaacataca caagcgagac tgacctatac
      541 caccaccaat agacaagggt aatgaacctt ctactaagcg tttgtggtaa taaaattgta
      601 aacgttcttc tgtatgtgtt atagctaatt gtttgatcaa cgcatcttta tctacgcgga
      661 tacccataga tgataattct aaaggcattt gcaaaacaga gtcccacacc aaaatatcac
      721 catttaatcc tgctaaccca ttttcgccta tggtagtcca atcatcatag tctggagcac
      781 gaccatcgtg tttttcacca ttggctaatt gccccccaat acccataata aacacggcac
      841 catgttcttt ggtaaccatg tattcgcgtt ctttgggtga tttatctgga tacattgcca
      901 acaattcttc ggcatggata aagaaaatat cgcgaggtaa tgacggtttg atagtaggaa
      961 aatattcata tacagcatat tcggcacgta ccattacgcc atatatacgt tttactactt
     1021 gttgtaaaaa tgcaatggtt ctgtcttctt ttgcaatcac catttcccaa tcccattggt
     1081 ctacatacaa agaatgtaga ttatctagct cttcatcaga gcgaatagca ttcatatctg
     1141 tatatacacc atagccattc tcaatagcat agtcggctaa aataatgcgt ttccactttg
     1201 ccaaagagtg tacaacctct gcttttacat cacccaaatc ctttacagga aaactaacag
     1261 cacgttctac cccatttaaa tcgtcgttaa tccccatacc ttgtaataca aacaagggag
     1321 cagttacacg acgtaaacgt agttcggcag aaaaactagt ttgaaaaaaa tctttcagaa
     1381 ttttaatagc caattctgtt tgttgcaaat ccaataagga tgtgtaagtt tgaggcacaa
     1441 tgagttttcc cataaaaaat tgcgtttttt taatagtggt tgcaaaatta cagaatgtta
     1501 ttgtaaaaaa agaacatata cctattttta ttagcaatat aacataaaaa acagcgaaag
     1561 aacgtacaaa aagacatcta gtgatcatta tttatttaca tacccatcag gataaattac
     1621 tatttgcctc tatcattaaa aaaaaagatg tatttttgca ccactttata tcacggtcct
     1681 atagtttaat ggataaaacg taagattccg gttcttatga tggaggttcg attcctccta
     1741 ggatcaataa agattgataa aaaaatctct cccctatctt cccttgtatt acacacattt
     1801 tacactgaaa attagcctca attattttgc tggctaaaaa aactttaata actttacgca
     1861 aatattttaa agaatataaa ccgaaggagt ggttgtttga tggagaaggt gaaggacaga
     1921 cttaagatat atacagagtt tgcttggtca ttcaagtagt aaaacaacgg agatatatac
     1981 acatattaca aacaagggat ttgatcaga
//
LOCUS       DAOPUR010000296         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_61017_length_2006_cov_2.157868, whole genome shotgun sequence.
ACCESSION   DAOPUR010000296 DAOPUR010000000
VERSION     DAOPUR010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61017_length_2006_cov_2.157868"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKN90_05655"
     CDS             <1..387
                     /locus_tag="PKN90_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85794.1"
                     /translation="EQQRGLKTILVPIENKQLQVGDKVIVRLTISTDRDMDFVHIKDL
                     RASGLEPVEQVSTYRYREGIGYYQNPKDTHTNFFFSQFPKGTYVIEYPLWVRFNGVYS
                     GGISTIQCLYAPEFQSHSFVQQLFVK"
     gene            394..915
                     /locus_tag="PKN90_05660"
     CDS             394..915
                     /locus_tag="PKN90_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HNZ85795.1"
                     /translation="MIRKEEIFEAGKFHKTHGVKGELSCSFSFPVDSSKLSFIVSNIE
                     GIYVPFFIEDMREKTSTSLLLKLKNINTDEDAKFLLDSPIYLPNELRKTDDEEVDVAY
                     FIHFTVVDANCGKIGVITAIDDSTPNTLFCVDTEQGETLYIPVAEEFFLSIDESTKTI
                     YMDLPTGLLTLNM"
     gene            1007..>2006
                     /locus_tag="PKN90_05665"
     CDS             1007..>2006
                     /locus_tag="PKN90_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNZ85796.1"
                     /translation="MSLLSQKLAKYDAPQKIQEAGVYPYFRPISSDQDTVVQINGKPV
                     LMFGSNSYLGLTNHPEIKQAAIKAVEKYGTGCAGSRFLNGTLDIHLELEARLAKLVGK
                     EEALVYATGFTVNSGVVSCLTGKEDYVILDKANHASIIEGSRLSFSKIIKYAHNDMDA
                     LEKQLKKCEPDKVKLIVVDGVFSMEGDEAKLLEIADLAKKYNASIMVDEAHSLGVYGN
                     HGAGICNHLGVTKDVDLIMGTFSKSLATIGGFIAADKHIINYLKHTSRTLIFSASITP
                     ASTACVLAALDVMEKETWRMDALWANTNYMRAKLQKAGFDTGHSTTPIIPLYVRDNT"
BASE COUNT          688 a          292 c          389 g          637 t
ORIGIN      
        1 gaacaacaac gaggattaaa aacaatactt gttccaattg aaaataagca attgcaagta
       61 ggagataaag tgattgttcg tttaacaata tctacagaca gggatatgga ttttgtacat
      121 ataaaagacc tacgagctag tggcttggag ccagtagagc aagtatctac atatcgttac
      181 agagagggaa ttggttatta tcagaaccca aaagataccc atactaattt ctttttcagt
      241 caattcccca agggtaccta tgtaattgaa tatcctttgt gggttcgttt taatggtgta
      301 tatagcggag ggataagcac aatacaatgt ttgtatgctc ctgaatttca gtcacattcg
      361 tttgttcaac aactatttgt aaaataataa aatatgatta gaaaagaaga aatatttgaa
      421 gcaggaaaat ttcataaaac acatggtgtt aaaggtgaat tgtcgtgttc attttcattt
      481 ccagtagata gtagtaaact atcgtttatt gtgagcaata tagagggtat ttatgtgcct
      541 ttttttatag aagatatgcg tgagaagaca tctacgtcct tactcttgaa attgaaaaat
      601 atcaatacgg atgaagatgc aaaattttta ttagattctc ccatttattt gcccaatgaa
      661 cttagaaaaa cagatgatga ggaagtagat gtggcatact ttatacactt tactgttgtt
      721 gatgcaaatt gtggtaaaat tggtgtaatt actgctatag atgattctac ccccaatacc
      781 ttattttgtg ttgatacgga acaaggcgaa accttatata ttcctgtggc agaagagttt
      841 ttcctttcaa tagatgaatc tacaaaaaca atctatatgg atttacccac tggattgctc
      901 acactcaata tgtaaataaa taattagtaa acttaattga aaataacaaa ataatcggta
      961 tatttgcact ctttttaaaa aatgtgtaac taaaattaac gcaagcatga gtttattaag
     1021 tcaaaagttg gcaaaatacg atgcccctca aaaaatacag gaggcaggtg tttatcctta
     1081 ttttcgtcct atctcttctg accaagatac cgttgtacaa atcaatggta aaccagtttt
     1141 aatgttcggt tctaatagtt atttagggct aactaatcat cctgaaatta aacaagccgc
     1201 aattaaagct gtagaaaaat acggtacagg ttgtgctggt tcacgttttt taaatggaac
     1261 attggatatt catcttgaat tagaagctcg tttagctaaa ttagtaggta aagaagaagc
     1321 attggtatat gcaacgggat ttactgtaaa ttcgggagta gtttcttgtt taacaggcaa
     1381 ggaagattat gtgattttag ataaagcaaa tcatgcatca attatagaag gaagccgttt
     1441 gtcattttct aagattatta aatatgctca caatgatatg gatgcattgg agaaacaact
     1501 aaaaaaatgt gagcctgata aagtaaaatt gattgttgtg gatggtgttt ttagtatgga
     1561 aggagatgaa gcaaaattac tcgaaattgc tgatttggca aaaaaataca atgcatcgat
     1621 catggtagac gaggctcatt cattaggagt gtatggcaat catggggcag gaatctgcaa
     1681 ccatcttggc gtaaccaagg atgtggactt aataatgggt acttttagta aatcgctagc
     1741 tacaattggt ggttttattg cagctgataa gcatattatt aactatctaa aacatacttc
     1801 tcgtacattg atattcagtg caagtattac acccgcttct accgcttgtg tattggcagc
     1861 cttagatgta atggaaaaag aaacttggag gatggatgct ttatgggcaa ataccaatta
     1921 tatgcgagct aaattgcaaa aagctggatt tgatacaggt cattctacta cgccaattat
     1981 tcctttgtat gtgcgtgata atacaa
//
LOCUS       DAOPUR010000297         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_61433_length_1996_cov_3.631633, whole genome shotgun sequence.
ACCESSION   DAOPUR010000297 DAOPUR010000000
VERSION     DAOPUR010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61433_length_1996_cov_3.631633"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1723
                     /locus_tag="PKN90_05670"
     CDS             185..1723
                     /locus_tag="PKN90_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNZ85797.1"
                     /translation="MFVRKKKNTSGSLSVQVIQKIKGKNKVIKTVGSATTQQKVDELV
                     LLAKQEIKKIENQQELFVSEKDTEADLLFETLSNASIRTLGPEIVFGKIYDFIGFGSI
                     KEDMFRHLVISRMAFPLSKLKTIQYLYRFQGVLLDIDAVYRFLDKLNSRLKEQVEQIA
                     FAHTKQVLQGNISVVFYDMTTLYFEASDEDDLRKTGFSKDGKHQNPQIYIGLLVGLGG
                     YAIGYDIFEGNTYEGHTLIPFLEKISKKFNLNKPIVIADAGLLSNENIKALEEKKYEY
                     IIGARLKNEPENIKNQILGNTYIDGSTISINKTKTTRLIVNYSSVRSAKDAHNRKRGL
                     SRLEKQIKSGKLTKSSINNKGYNKYLKMTGDVAIEIDYQKYNLDNKWDGLKGYVTNTR
                     LSNNEVMENYKNLWHIEKAFRMSKTDLRIRPIYHRLRNRIEAHICISFTAYCIYKELE
                     RVLYDEKSSISLKKASELTHNMYQITYMLPDSKITKSRLLTMDEEQNELYKIINKNFR
                     VSQR"
BASE COUNT          746 a          288 c          343 g          619 t
ORIGIN      
        1 atggcgttta ctataataat atcatccctt cgggattaaa aaaactatcg acatttagac
       61 attcagacaa tgagacattc agacatttag acctcctgtt ttccgcactt tttcggggca
      121 cccacataac tcattgtaaa tcagaatatg ttttgtattt ttgggtgccc caaaaatttg
      181 aaacatgttt gttaggaaaa agaaaaacac ctctggaagc ctttctgtac aagtgataca
      241 gaagattaaa ggcaagaata aagtaataaa aaccgttggt tctgccacca cacagcagaa
      301 agtagatgaa cttgttttgt tagcaaaaca agagataaaa aagatagaaa atcaacagga
      361 attgtttgtt tcagagaaag atacagaagc cgatttgctc tttgaaaccc tttcaaatgc
      421 aagtattaga actcttggac ccgaaatagt ttttggtaaa atttacgatt ttattggatt
      481 tggtagtatt aaagaagata tgtttcgtca tttggttatt tcgcgaatgg cttttccttt
      541 aagtaaacta aaaactatcc aatatttata tcgatttcaa ggggttttac ttgatattga
      601 tgcagtttat cgttttttag ataaactcaa ctctcgatta aaagaacagg tagaacaaat
      661 tgcatttgca catacaaaac aggtattaca gggaaacatt agtgtggttt tttatgatat
      721 gacaacattg tattttgaag ccagtgatga agatgattta cgtaaaacag gtttttctaa
      781 agatggtaaa catcaaaatc cacaaattta tattggactt ctcgttggac ttggtgggta
      841 tgccattgga tatgatattt ttgaaggaaa tacatatgaa ggacatacgt taataccttt
      901 tttagaaaaa atcagcaaga aatttaatct aaataaaccc attgttattg ctgatgcagg
      961 attgctttca aacgaaaata taaaagcact ggaagaaaag aaatatgaat atataattgg
     1021 agctcgatta aaaaatgagc cggaaaatat taaaaatcaa attcttggaa atacttatat
     1081 tgatggttca acaatcagta ttaataaaac taaaactacg aggcttattg ttaattattc
     1141 aagcgttcgt tctgcaaaag atgcccacaa tcgcaaacga ggactttcga ggcttgaaaa
     1201 gcaaataaaa tcgggaaaac tcacgaaatc tagtataaac aataaaggat ataataaata
     1261 tcttaaaatg actggtgatg ttgctattga aattgattat caaaaatata atttggacaa
     1321 taaatgggat ggattgaaag gttacgtaac aaatacgagg ctttcgaaca atgaagttat
     1381 ggaaaactac aaaaatcttt ggcatatcga aaaagctttt agaatgtcta aaacagattt
     1441 gcgaatacgt ccaatttatc atagattacg caacagaata gaagcacata tttgcatatc
     1501 ttttactgca tattgcattt acaaagaatt ggaaagagtg ctttatgacg aaaaatcaag
     1561 catatcattg aaaaaagctt ctgagcttac tcataatatg tatcaaataa cttatatgtt
     1621 acctgattca aaaatcacta aaagtcgctt attaacaatg gatgaggaac aaaatgaact
     1681 atacaaaata atcaacaaaa attttagggt gtcccaacgc tgaaaacagg aaatttgtct
     1741 ttcaaaaatg ctaattttta taaaattata ctttatatac ttgctgtaga aatttgcttc
     1801 ttcttatttt ttctaaagca aaaaagtaat tcgacagggc gaaccgccat gcaattcata
     1861 tctctaaaga tttgtagatt tttttttgaa ttgtgcaata cgctttggta taacttcagc
     1921 ggctacgagc atgcgtgtgt gagaggaaag ctcaaaactt agaaacattt agaaagctgc
     1981 tttagtcacg tggttt
//
LOCUS       DAOPUR010000298         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_61562_length_1993_cov_2.883385, whole genome shotgun sequence.
ACCESSION   DAOPUR010000298 DAOPUR010000000
VERSION     DAOPUR010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61562_length_1993_cov_2.883385"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKN90_05675"
     CDS             <1..470
                     /locus_tag="PKN90_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNZ85798.1"
                     /translation="FVRRNRIIAGTTDATLIVESAARGGAIITTEIANSYNRDVFAVP
                     GKCTDEWSVGCNNLIKQHKAALVNSASDIEYIMGWTKEQTSQKIEQQQSLFVDLSDTE
                     QEIFTIIKQNKVVHIDVLAIKISKSIKDLSPILLSMEFKGVIKCLPGNIYSIV"
     gene            1063..1986
                     /gene="nadA"
                     /locus_tag="PKN90_05680"
     CDS             1063..1986
                     /gene="nadA"
                     /locus_tag="PKN90_05680"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNZ85799.1"
                     /translation="MTLQEKITKLKKEKNAIIMAHYYQTAEIQDIADFVGDSLALAQW
                     AASTDAAIIVLCGVNFMGETAKILSPNKKVLIPDMEAGCSLADSCKASDFEKFIQQHP
                     DHIVISYVNTTAEVKALTDVVVTSTNAHKIVDSFPKEQKIIFGPDKNLGNYINSVTGR
                     NMVLWDGACHVHEQFSLEKILALKKQYPEAQILAHPECKKVVLTVADYIGSTSALLAY
                     TGTSDCNVFIVATESGILHKMKQQNPTKTFIPAPPNDSTCACNECNFMRLNTLEKLYN
                     CLLNETPEIQVEETIRQKAVKPILKMLEMSK"
BASE COUNT          760 a          306 c          319 g          608 t
ORIGIN      
        1 attttgtacg tcgaaatcgc attatcgcag gtacaaccga cgctacactt attgttgaaa
       61 gtgctgcgcg tggtggtgct attattacta ccgaaatagc taattcatat aacagagatg
      121 tgtttgctgt acctggtaaa tgtactgatg aatggtctgt aggttgtaat aacctaataa
      181 aacagcacaa agcagcatta gtgaatagtg cttctgatat tgaatatatt atgggttgga
      241 caaaagaaca aacaagccaa aaaatagaac aacaacaatc tttgtttgta gatttatccg
      301 atacagaaca agaaattttt acaattatca aacaaaataa agtcgttcat atagatgtgt
      361 tggcgataaa aataagcaaa agcataaaag atttatcgcc aatattgctt tctatggaat
      421 ttaaaggtgt aataaaatgt ttaccaggta atatttactc gatagtataa acacaaaagg
      481 gactaatacc ttgctagtat taatcccttt ttattaacct taaatcaaat actttgaaaa
      541 tcacactaca aaagtatact gatttatagg atattgtatc aaatttaagc ataatttgca
      601 taattattta cgcaagcgtt tgcactgtta gtaatactgt acttattgtt taaaagctgt
      661 taataaaata gagagtaaaa aatgataaaa aaatttgtag ttgtaaatta tgatgtttac
      721 tatacaaata ttttataaaa caagaatata gttatgtatt ttaaaaaaaa gtaatgaaca
      781 ataaaaagag cagttattaa ccataaaaaa acgaaaagtt tactacattt gtagttgtaa
      841 acaatatgtt aataacctta tcaatacatt ttttatcagc atattatact gttaattatc
      901 tgttgaaata ctctgtatat tacaccgtat tttaataatg caaattttta gtgttttatt
      961 tataaacctt atcaacaccc tattattctt atcattcttt tttataactt ttttaaataa
     1021 ataaaagaat aaagaatact gttgataata acaataacga aaatgaccct acaagaaaag
     1081 attacaaaac tcaaaaaaga aaagaatgcc attattatgg ctcactacta ccaaacagcc
     1141 gaaatacaag atattgccga ttttgtgggt gatagtttgg cattggcaca atgggctgct
     1201 agtaccgatg ctgccattat tgtattatgc ggggtaaatt ttatgggaga aacagctaaa
     1261 atattgtctc caaacaaaaa agtgctaata cccgatatgg aagctggatg ctcattggct
     1321 gatagttgta aagcaagtga ttttgaaaaa tttatacaac aacaccccga tcatatagtg
     1381 atatcttatg taaatactac cgctgaggta aaagctttaa ctgatgtagt agtaacatct
     1441 accaatgcac ataaaatagt ggatagcttt ccgaaagagc aaaaaatcat ttttggacca
     1501 gataaaaact taggaaacta catcaatagt gttacaggta gaaacatggt gttgtgggat
     1561 ggagcttgtc atgtgcacga acagttttcg ttagaaaaaa tacttgctct taaaaaacaa
     1621 tacccagaag cccaaatttt agctcatcca gagtgtaaga aggttgtgct taccgtagcc
     1681 gattatattg gttctacctc tgccctatta gcttatacag gcacttcaga ttgcaatgtg
     1741 tttatagtag ctaccgaaag tggtattttg cacaaaatga aacaacaaaa ccccaccaaa
     1801 acatttatac ctgcaccacc taacgatagt acctgtgctt gcaacgaatg taactttatg
     1861 cgacttaata ccctcgaaaa attatataac tgtctactaa acgaaacacc cgaaattcaa
     1921 gtagaagaaa ccatcagaca aaaagccgta aaacccattt tgaaaatgtt ggaaatgagt
     1981 aagtaggagg aat
//
LOCUS       DAOPUR010000299         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_61708_length_1990_cov_3.192248, whole genome shotgun sequence.
ACCESSION   DAOPUR010000299 DAOPUR010000000
VERSION     DAOPUR010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61708_length_1990_cov_3.192248"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1228
                     /locus_tag="PKN90_05685"
     CDS             245..1228
                     /locus_tag="PKN90_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85800.1"
                     /translation="MKKSILATFVFFAFLAVSHAQESVPFKMSAKPTITLFSNFKTGI
                     ADPNNKIGFNLERVYLGTSFVLSENLSGRVRFDIGDPKVPGSDLEMVAYVKNAEIKWQ
                     AGNVTFNAGMISTIQFAVQEKFWGYRYLLKSFQDEYKFGPSADLGIAASYKISDLITI
                     DAIAINGEGYKKVNKDDALRYGGGVLISPSEWILLRAYYDRNDVATGAIGATQENVGL
                     FAGISLQNITLGAEYNTLYNVDRTRGNHQYGYSVYSTVVLPKQFQLYGRWDYLFSSNE
                     YNLAKDGQLGVLGVQYSINKYVQVSPNLRIWQSAEGGAAKLCGLVNLQVNF"
     gene            complement(1297..1620)
                     /locus_tag="PKN90_05690"
     CDS             complement(1297..1620)
                     /locus_tag="PKN90_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ85801.1"
                     /translation="MTKHIFSPKRYLSSFLFVFFFFLSIVISFAQSITGTVIDKKTKE
                     TLAGAAIAVNGQKVYTDLDGNFTLANLGGSKCELTVSMISYETQTIEMDLAKNKDVAI
                     VLKKR"
     gene            complement(1786..>1990)
                     /locus_tag="PKN90_05695"
     CDS             complement(1786..>1990)
                     /locus_tag="PKN90_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85802.1"
                     /translation="DSTVTKQLQKTDYKGFVISEYNFSSVTNTESLERYFEFYRTNNL
                     QITEPNTIKQLEQESGFVKGNAE"
BASE COUNT          649 a          285 c          383 g          673 t
ORIGIN      
        1 tacagctgct taacaaaact gtaacagtgc tcaataaaaa tactagtttt ttcatgtttt
       61 ctgttgctaa atgtttaatt aaatatgaat aaaaagttta caatgcaaag aaacaactaa
      121 cttgttgcgg aaatgttacg caaatgttac gaagatgtta agatttgaaa ggatagaatg
      181 gtgcgtgtaa caagataaaa aaactttata tacttttgcc tacgaaataa ataattatca
      241 ggatatgaaa aaatcaattt tagctacatt cgtatttttt gcttttttag cagtgtctca
      301 tgcacaagag tctgtccctt ttaaaatgag tgcaaaacct accattactc tatttagtaa
      361 ttttaaaacg ggaatagcag atccgaataa taaaataggt tttaatttag aaagagttta
      421 tctgggcaca tcctttgtat taagtgaaaa cttatctggt cgcgtacgtt ttgatatagg
      481 agatcccaaa gttcctggtt ctgacttaga aatggtggcg tatgtaaaaa atgcagaaat
      541 taaatggcaa gctgggaatg taacattcaa tgcgggtatg attagcacta ttcaatttgc
      601 tgttcaagaa aagttttggg gttaccgcta tttacttaaa tcgtttcaag atgagtataa
      661 atttggtccc agtgctgatt tggggattgc tgcaagttac aaaatatcag atttgataac
      721 gatagatgcc atagctatta atggagaagg ctacaaaaaa gtaaacaaag atgatgcatt
      781 acgttacgga ggaggagtac ttatttcacc ttcagaatgg attttgttgc gtgcgtatta
      841 tgatagaaat gatgtagcta ctggagctat aggagccaca caagaaaatg taggtttgtt
      901 tgcaggaatt agcttacaaa atatcacact aggagccgaa tataatacat tgtataatgt
      961 ggatagaaca agaggcaatc atcaatatgg ctattcggtg tatagtacag ttgtattacc
     1021 aaaacaattt cagctttatg gtcgttggga ttacttattt tcgagtaatg agtataacct
     1081 agcaaaagac ggtcagttag gagtattggg tgttcaatat agtataaata aatatgtcca
     1141 agtttcgcca aatcttagaa tatggcaatc agctgaaggt ggagcggcta agctttgtgg
     1201 tttggttaat ttgcaggtta acttttaggt aaaaaggata aagagcgatg aaaaaatttt
     1261 ttcatcgctc tttatccttt tatcaatcaa taccctttat ctttttttca atacaatagc
     1321 tacatcttta tttttagcca aatccatttc gatggtttgt gtttcgtacg aaatcatact
     1381 cactgtaagt tcgcatttgc taccacctag gtttgctagg gtaaaattgc catctaagtc
     1441 ggtgtatact ttttgaccat ttaccgcaat tgctgctcct gcaagagttt cttttgtttt
     1501 tttatcaata acagttccag taatactttg agcaaaactt atgactatgg ataaaaaaaa
     1561 gaaaaaaaca aataggaatg atgataggta tcgtttcggc gaaaaaatgt gttttgtcat
     1621 attgcagtta agtttaggta taggtcgttt ttgataaacg aatctatttt acaaccgcaa
     1681 aggtaaaaac tgggtgttgc gttcttgtta cggaatgatt acggacttgt gacgattttg
     1741 ttcggggaaa aaccagctct tttacatgtc gaatttgctg aactgttatt cggcgttacc
     1801 ttttacaaac cccgattctt gctctaattg cttgatggta ttaggctctg taatttgtag
     1861 gttattggta cgataaaatt caaaataacg ttccaatgat tctgtatttg taacagaaga
     1921 aaaattatat tcagaaatta caaaaccttt gtaatcggtt ttttgtaatt gttttgtaac
     1981 agtactatct
//
LOCUS       DAOPUR010000300         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_62152_length_1981_cov_3.542056, whole genome shotgun sequence.
ACCESSION   DAOPUR010000300 DAOPUR010000000
VERSION     DAOPUR010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62152_length_1981_cov_3.542056"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1981)
                     /gene="uvrA"
                     /locus_tag="PKN90_05700"
     CDS             complement(<1..>1981)
                     /gene="uvrA"
                     /locus_tag="PKN90_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNZ85803.1"
                     /translation="FFSKKLMCPTTGISYNEPAPHDFSFNSPKGCCPKCNGVGVITKI
                     DSSKIIPDDSLSIYDGAIAPLGKFKNGILFLQIQTLCEQYDCTLKTPIKKLPSELVDK
                     IIYGTSDKLFIKSETAGLSNYPIAFDGIVHYVTMQQDSDASATAQKWAEQFSETVVCP
                     ECNGTRLKKEALFFKILDKNIAELSAMDIEQLHSWIQLNTQHFDERQKVIATEICKEI
                     TTRLQFLLNVGLTYLSLNRPSMSLSGGESQRIRLATQIGSQLVNVLYILDEPSIGLHQ
                     RDNLKLIDSLKQLRDMGNTVIVVEHDKDMMLAADYVVDLGPKAGRLGGELVFAGTPSE
                     MLRAHTLTSDYITGVRKIEIPPKRRDGNGNYLRIKGAKGNNLKGVDVSFPLGMFICVT
                     GVSGSGKSSLINGTLYRILSQHFYHSLKNPLPYDTVEGLEHIDKVIQIDQSPIGRTPR
                     SNPATYTGIFSDIRNLYVNLPEAQIRGYKPGRFSFNVKGGRCETCKGNGYNRLEMSFL
                     ADVYVPCPDCHGKRYNRETLEVRFKGKSIADVLNMTINQAVEFFDNIPTIVHRLRVLQ
                     DVGLGYIKVGQPSTTLSGGESQRVKLATELAKKDTGQTMYILDEPTTGLHFEDINVLL
                     GVLNRLVDKGNTLVVIEHNLDVIKCADHIIDIGLEG"
BASE COUNT          579 a          419 c          347 g          636 t
ORIGIN      
        1 accttcaagt ccaatatcaa tgatatgatc ggcacatttt atcacatcta agttatgttc
       61 aattacaact aaggtattgc ctttgtcaac caatcgattc agtacgccta gtaacacatt
      121 aatatcttcg aaatgcaaac ctgtagttgg ctcatccagt atatacatgg tttgtcctgt
      181 gtcttttttt gccaattcgg tggctagctt tactcgctga ctttcgcctc ccgataaggt
      241 ggtagagggt tgccctactt tgatataacc tagtcctaca tcttgcaaca cacgcaagcg
      301 atgaacgatg gttggtatat tgtcaaaaaa ttctaccgcc tgattaatcg tcatattcaa
      361 gacatctgcg atggatttcc ctttgaaccg cacttccaat gtttctcggt tataccgttt
      421 tccatgacaa tcaggacaag gcacatacac atctgccaaa aaactcattt ctaagcgatt
      481 atacccgttt cctttgcagg tttcgcacct accgcctttt acgttaaacg aaaaacgacc
      541 tggtttatat ccacgtattt gtgcttcagg caaattaaca tacaaattgc gtatatccga
      601 aaatatacct gtataggtcg ctggattcga acgaggtgta cgtccgatag gcgattggtc
      661 aatttgtata actttatcaa tatgttccaa tccctctaca gtatcatacg gtagcggatt
      721 tttaagagaa tgataaaaat gttggcttaa aatgcgatac aaggtaccgt taattaagct
      781 agattttccg cttcccgata cacccgtaac acaaataaac attcctaatg ggaaagacac
      841 atcaacccct tttaggttat tcccttttgc tcctttaatt cgcaaataat tgccgtttcc
      901 atcgcgtctt ttagggggta tttctatttt cctaactcct gtaatataat cagaagttaa
      961 ggtatgtgca cgcagcattt ccgagggagt tcctgcaaaa accaactcac cacctaatcg
     1021 tccagctttg ggacctaaat caaccacata atcggctgcc aacatcatgt ctttgtcgtg
     1081 ttctacaaca atgacggtat ttcccatatc acgtaattgc tttagcgagt ctatcagttt
     1141 taaattatct cgttggtgca atccaatact gggctcgtct aaaatataca gcacattgac
     1201 taattgagac ccaatctgcg ttgccaaccg tatgcgttgg ctttcgcctc ccgacaaact
     1261 catggaagga cgattaagag agaggtaagt aagtcccaca tttaataaaa actgtaacct
     1321 tgtggtaatt tctttacaaa tttctgtggc aattactttt tgtctttcat caaaatgttg
     1381 tgtgttgagt tgtatccaac tatgcaattg ctcaatatcc atagcagaca attcagcaat
     1441 atttttatcc aaaattttga aaaacaaggc ttctttttta aggcgtgtac cattacactc
     1501 tgggcatacg actgtttctg aaaattgttc agcccacttt tgagcagttg cagaggcatc
     1561 gctatcttgt tgcatggtta catagtgcac aatgccatca aaggcaatag gataattaga
     1621 taagcctgct gtttcgcttt tgataaataa tttatcggat gtgccataaa taattttatc
     1681 cactaattct gagggcaatt tttttatagg tgttttcagc gtacaatcgt attgttcgca
     1741 taatgtctga atttgtaaaa ataatatccc atttttgaat tttcctaagg gtgcaatggc
     1801 accatcataa atagaaagag agtcgtcagg aataatttta ctactatcta ttttggtaat
     1861 aacacctacc ccattacatt ttgggcaaca gcctttgggc gaattaaaag aaaaatcgtg
     1921 aggagcaggc tcattgtacg aaattccagt agtcggacac attaattttt tgctaaaaaa
     1981 c
//
LOCUS       DAOPUR010000301         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_62353_length_1977_cov_3.349116, whole genome shotgun sequence.
ACCESSION   DAOPUR010000301 DAOPUR010000000
VERSION     DAOPUR010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62353_length_1977_cov_3.349116"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKN90_05705"
     CDS             complement(<1..691)
                     /locus_tag="PKN90_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HNZ85804.1"
                     /translation="MEQVLIVVEKFFEDASSFLWGIPMIVLLFGTHLYLTVRTGFVQR
                     KIGTAIKLSFTSDKESAGDISQFGSLVIALAATIGTGNIIGIATAIALGGPGAVFWCW
                     LTGVFGIATKYSESLLAVKYRVKTESGTMIGGAMVVLDKVLKMRWLAVLFCVFTAIAA
                     FGIGNMTQANSISVLVEQHFAIPPYVTGIILAVSVGIVMFGGVKWVATVCKALVPTMA
                     LVYIIGCLLLLG"
     gene            883..1620
                     /locus_tag="PKN90_05710"
     CDS             883..1620
                     /locus_tag="PKN90_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ85805.1"
                     /translation="MNLITHVGRYSQLMTGVFKKPDKWHITLRQIVKEINKLGVDSLG
                     ITIIISVFIGSVITLQTVLNTANPLLPTYVTGLVTRDTLLLEFSSTILCLILAGKVGS
                     NIASEIGTMRITEQIDALKIMGVNAPNYLIFPKIIAFCLIVPVLVIFSMFTGIVGGYI
                     ITYTGVISPNDYILGIQYAFIPYYIVYSIIKSVIFAFIITSVSAYYGYFSYGGALDVG
                     KSSTNAVVNSSIIILLFNVLLTDLFLM"
     gene            1623..>1977
                     /locus_tag="PKN90_05715"
     CDS             1623..>1977
                     /locus_tag="PKN90_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ85806.1"
                     /translation="MIEVKNVCKSFDGVEVLKNVSALFHAGKTNLIIGQSGSGKTVLM
                     KSIIGIHHVTSGQILYNNRDFTAMSGDEVKALRQEVGMLFQGSALFDSMTVEENVRFP
                     LEMFSNLPKEAWDDRV"
BASE COUNT          657 a          384 c          331 g          605 t
ORIGIN      
        1 aaccaaggag taacaaacat ccaataatat ataccaaagc catcgtaggt actaatgctt
       61 tgcacacagt tgctacccat tttacaccac caaacatcac tatcccaaca cttactgcca
      121 atataatacc tgtaacatac ggaggtatag caaaatgctg ttctaccaat accgagattg
      181 aatttgcttg tgtcatgtta ccaataccaa aagcagctat ggctgtaaaa acacaaaata
      241 acacagccaa ccatctcatt tttaaaacct tatctaaaac taccatagct ccaccaatca
      301 ttgttcccga ttcggtttta acccgatatt ttacagctaa tagcgactcc gaatatttgg
      361 tagcaatacc aaaaacacct gttagccaac accaaaaaac agcacctgga ccacctaagg
      421 caatggctgt agcaatgcca ataatattgc ctgtgccaat agtagctgct agggctatta
      481 ccagcgaacc aaactgactg atatctcctg ctgattcttt atcagaagta aacgataatt
      541 taatagcagt acctatcttt cgttgtacaa aacctgtacg aacagtaaga tacaagtgtg
      601 taccaaacaa caacacaatc atagggatac cccacaaaaa agaacttgca tcttcaaaga
      661 atttttctac aacaattaag acttgttcca tacaacttat ttattttatt atcaattact
      721 tacaataacc ctacacccag ttaataaaaa atagaaagac gatactgcaa acaatacact
      781 aatggcgtat tgcttgttgc gacaaaagta taataaatat taacaaaata agaataaaaa
      841 aataattacc tttgtgggtt gaactatttt taagcaaaat ctatgaatct gattacacac
      901 gttggtaggt attctcaatt aatgacagga gtttttaaaa aaccagataa atggcatatt
      961 accctccgac aaatagtaaa agaaataaac aaattaggtg ttgattcgtt aggcatcacc
     1021 attatcatat cggtgtttat tggttcggtt attaccttac aaacggtttt aaatacagct
     1081 aacccattgc tacctactta cgtaactggt ttagtaactc gagacacctt attattggaa
     1141 ttttcatcta ctattttatg cctcatttta gcgggaaagg ttggttctaa tattgcttcg
     1201 gaaattggca ccatgcgtat taccgaacaa attgatgcct taaaaattat gggggtgaat
     1261 gctcctaact accttatttt tccaaaaata attgcttttt gtttaattgt gcctgtattg
     1321 gttattttta gtatgtttac aggtattgtg ggtggctata ttatcacgta taccggtgtt
     1381 atttctccaa acgattatat cttgggtatt caatacgcgt ttatccctta ctacattgta
     1441 tattcaatca ttaaatcggt aatttttgcc tttatcatta catcggtgtc ggcgtattac
     1501 ggatatttct catacggagg agctcttgat gtgggcaaat cgagcaccaa tgcagtagtt
     1561 aacagcagca ttattatctt gctattcaat gtattattaa ccgatttatt cttgatgtag
     1621 ccatgattga agtaaaaaac gtatgcaaat catttgacgg tgttgaggta ttaaaaaatg
     1681 tttcggcact atttcatgct ggcaaaacaa acttaattat cgggcaaagc ggttcgggga
     1741 aaacggtact aatgaaaagt attattggta ttcatcacgt tactagcggt caaattttat
     1801 acaataaccg cgattttaca gctatgagtg gcgacgaagt aaaggcattg cgtcaagaag
     1861 taggcatgct gtttcaaggt tcggctctgt tcgactccat gacggtagaa gaaaatgtgc
     1921 gttttccgtt ggaaatgttt tctaacctgc ccaaagaagc ttgggacgac cgcgtaa
//
LOCUS       DAOPUR010000302         1964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_62962_length_1964_cov_3.291776, whole genome shotgun sequence.
ACCESSION   DAOPUR010000302 DAOPUR010000000
VERSION     DAOPUR010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62962_length_1964_cov_3.291776"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKN90_05720"
     CDS             <1..310
                     /locus_tag="PKN90_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNZ85807.1"
                     /translation="TERSLFEWAKEAQERGAGEILFTSMTHDGVKTGYANDALAQLSD
                     MLKIPIIASGGAGSKNHFKDAFLQGKADAALAASVFHFGEINISELKDYLRKHDICVR
                     "
     gene            328..921
                     /gene="hisIE"
                     /locus_tag="PKN90_05725"
     CDS             328..921
                     /gene="hisIE"
                     /locus_tag="PKN90_05725"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNZ85808.1"
                     /translation="MSLDFNKQGGLIPAIIQDSITNKVLMLGYMNEESLAKTKELGKV
                     TFYSRSKNRLWTKGEESGNFLHVVSIAEDCDNDTLLIKVNPVGPVCHTGADTCFFENN
                     VEDIMFLKYLQDFIDKRKQEMPEGSYTTSLFKKGINRMAQKVGEEAVETVIEATNGTD
                     EGFLYEASDLIYHLIVLLSSKGYRIEDLANELKKRHK"
     gene            931..1629
                     /locus_tag="PKN90_05730"
     CDS             931..1629
                     /locus_tag="PKN90_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ85809.1"
                     /translation="MGNTLIRYKGVEICQKEQVVLKDVNLEIGEGEFYYLIGKVGTGK
                     SSLMKTMYAELPIETGEASLLDYDLLTITKSEIPFLRRQLGIIFQDFQLLIDRTVNEN
                     LLFVLKATGWKDKEEMQARIDAVLKEVGMSNKGYKMPHELSGGEQQRIVIARALLNKP
                     KLILADEPTGNLDPLTGAELVELLYTICKEENTTIIMATHNLGWIDAFPGKVLAISQN
                     KLCSGTKDECIFDY"
     gene            1729..>1964
                     /locus_tag="PKN90_05735"
     CDS             1729..>1964
                     /locus_tag="PKN90_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ85810.1"
                     /translation="MKILVVGANSYAGAAIYTQLREKYEVVGTYNAYPLFEELIQLDI
                     THAEEVERVIKLHAPDTIVHVASNSNGNWCEQHP"
BASE COUNT          687 a          279 c          435 g          563 t
ORIGIN      
        1 gaccgaaagg agtctttttg aatgggcaaa agaagcacaa gaacgaggag ctggcgaaat
       61 tttatttacg agtatgacac acgacggggt gaaaacgggc tatgcgaatg atgccttggc
      121 tcaattgtcg gatatgttaa aaattcctat tattgcgtcg ggaggagcgg gtagtaaaaa
      181 ccatttcaaa gatgcattct tacaagggaa agcagatgcc gcattagcag caagtgtgtt
      241 tcattttgga gaaattaaca tttcagaact aaaagattat ttaagaaaac acgatatttg
      301 tgttcgataa aaaaaacgat taatacaatg agtttagatt ttaacaaaca aggcggctta
      361 attcccgcta ttatacaaga cagtattacc aataaagtgt tgatgttggg ctatatgaac
      421 gaagaatcat tagctaaaac aaaggaattg ggaaaggtta ctttttatag ccgtagtaaa
      481 aaccgcctgt ggacaaaagg agaagaaagt ggcaattttt tgcatgttgt atctattgct
      541 gaagattgtg ataatgatac cctattgata aaagtaaatc ccgtaggacc tgtatgccac
      601 acgggtgcag atacctgttt ttttgagaat aatgtagaag atattatgtt cttgaaatat
      661 ttacaagatt ttattgacaa acgcaaacaa gaaatgccag agggttcata taccacttct
      721 ttgtttaaaa aaggaataaa ccgtatggct cagaaagtag gcgaagaggc tgtagaaaca
      781 gtgattgagg ctaccaatgg tactgatgaa ggatttttgt acgaagcatc ggatttaata
      841 tatcacttaa ttgtgttatt gtcatcaaaa ggatatcgta tagaagattt agccaacgaa
      901 ttgaaaaaaa gacataaata ataaattgct atgggaaata cactcatacg ctacaaaggc
      961 gtagaaattt gtcaaaaaga acaagttgtg cttaaagatg taaaccttga aataggagaa
     1021 ggggagttct attacttgat aggaaaagta ggtacaggaa aaagtagttt gatgaaaact
     1081 atgtatgctg aattgcctat tgaaacgggt gaagcaagct tgttggacta tgatttatta
     1141 acgataacaa aatcagaaat tccattttta cgtcgtcagt taggtattat tttccaagat
     1201 tttcagttgt tgattgaccg tacggtaaac gaaaatttac tgtttgtgct taaagctact
     1261 ggctggaaag ataaagaaga aatgcaagca cgtattgatg ctgtattaaa agaggtaggt
     1321 atgagcaaca aaggctataa aatgccccac gaattatcag gaggagaaca gcaacgtatt
     1381 gtaattgctc gtgcattgct caacaaaccc aaattgattt tggcagatga gcctactgga
     1441 aacctcgatc cgctaacggg tgcagagctt gtggaattgc tgtatacaat ttgtaaagaa
     1501 gagaacacta cgattattat ggctacacat aatttgggtt ggatagatgc tttccctggt
     1561 aaggtgttgg ctatctctca aaataaattg tgtagtggca ccaaagatga atgtattttt
     1621 gactattaat ggttgtttac accaatacaa tgaagggagt ttttctccct ttttttgttt
     1681 gtaattgttg tatctgtgta agaaaatgaa tgcgtaataa acatagaaat gaagatttta
     1741 gtagtaggag ccaatagtta tgcaggagca gctatttata cccaattaag agaaaagtat
     1801 gaggtagtag gcacgtataa tgcgtatcct ttattcgaag aattgataca actggatatt
     1861 acacatgccg aagaggtgga gcgagtaata aaattgcatg cacctgatac tattgtacat
     1921 gtagcgtcta actccaatgg caattggtgc gagcaacatc caga
//
LOCUS       DAOPUR010000303         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_63424_length_1955_cov_3.557368, whole genome shotgun sequence.
ACCESSION   DAOPUR010000303 DAOPUR010000000
VERSION     DAOPUR010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63424_length_1955_cov_3.557368"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKN90_05740"
     CDS             <1..741
                     /locus_tag="PKN90_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNZ85811.1"
                     /translation="RIHTIVVSTQHDDFLTPTDTLTQEEADKQMLETIYNDVKNILLP
                     RVIAKDEQYASLFHNNIVLHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGA
                     FSGKDPSKVDRSAAYATRYIAKNIVAAGIADEILVQVAYAIGVAKPMNIYVNTYGSAK
                     VNKSDAEIASIIETIFDLRPAKIEHMLALRNPIYQETAAYGHMGRTPRTITKEFEISG
                     GKKISQTVELFTWEKLDKVDTIKKAFGL"
     gene            760..1746
                     /gene="dusB"
                     /locus_tag="PKN90_05745"
     CDS             760..1746
                     /gene="dusB"
                     /locus_tag="PKN90_05745"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNZ85812.1"
                     /translation="MKIANVEFPPYPLFLAPMEDVTNPSFRLLCKQYGADMVYTEFIS
                     ADALIRNVQRTEQKLHISQDERPVAIQLYGREPDAMAEAARIAETAQPEIIDLNFGCP
                     VKKVAGKGAGAGLLRDIPKMLAITKSVVNAVSIPVTVKTRLGWDDSSRIIVELAEQLQ
                     DCGIAALTIHGRTRAQMYTGNADWQLIGEVKNNPRMHIPIIGNGDVTTPERAKECFDK
                     YGVDAVMVGRACIGKPWIFQEIRHYLQTGEHIPQIPFTEQLSILKDQVERSIEWLDER
                     RGILHIRRHLAATPLFKGIPNFKDTRIAMLRATTKDELFAIFNSIETMVVER"
BASE COUNT          638 a          401 c          398 g          518 t
ORIGIN      
        1 cgcatacata ccattgttgt atctactcaa cacgatgatt ttttaacacc aaccgataca
       61 cttacacaag aagaagcgga caaacaaatg ttagaaacca tctataatga tgtgaaaaat
      121 attttacttc cacgcgttat tgccaaagac gaacaatatg cctcattgtt tcacaacaat
      181 attgtgttgc atgtgaatcc aacaggtaaa tttgtaattg gcggtccaca cggtgataca
      241 ggtttaacag gtagaaaaat tatcgtagat acatacggtg gcaaaggagc acatggtggt
      301 ggtgcttttt ctggaaagga cccctcaaaa gttgaccgct ctgctgccta tgctacacgc
      361 tatattgcca aaaatattgt ggcagctggc attgccgacg aaattttggt acaagttgct
      421 tatgccattg gtgtagcaaa acctatgaat atttatgtaa acacgtatgg ttcagccaaa
      481 gtaaacaaat cagatgccga aatagcgagc attattgaaa caatctttga cctacgacca
      541 gcaaaaattg aacacatgtt agctttacgc aaccccatat accaagaaac tgcagcttac
      601 ggacacatgg gacgcacacc acgcaccata acgaaagaat ttgaaatatc tggtggcaag
      661 aaaatcagcc aaacagtaga actatttact tgggaaaaac ttgataaggt tgacacgata
      721 aaaaaagcat ttggtttgta atccatttat ttgtacacca tgaaaattgc taacgtagaa
      781 tttcctcctt atcccttatt tttggcacct atggaggatg ttaccaaccc gagctttagg
      841 ttgttgtgta agcaatatgg tgcagacatg gtatataccg aatttatatc ggctgatgcg
      901 ttgatacgca atgtgcaacg aacagaacaa aaattgcaca tcagccaaga cgaaagacca
      961 gtggctattc aactatacgg cagagaaccc gacgctatgg cagaggctgc acgtatagct
     1021 gaaacagcac aacccgaaat tattgatctg aattttggtt gtccagtaaa gaaagtggca
     1081 ggcaaaggag caggtgcagg tttgctacgt gatattccga aaatgttggc aattaccaaa
     1141 agtgttgtca atgcagtatc tattcctgta actgtaaaaa ctcgccttgg gtgggacgat
     1201 agtagccgta ttattgtaga attggcagag caattacaag attgtggcat agcagcactc
     1261 accattcatg gacgcacacg ggcacaaatg tacacgggta atgccgactg gcaactaatt
     1321 ggcgaagtga aaaacaaccc ccgcatgcat atccccatta ttggtaatgg tgatgtaact
     1381 accccagaaa gggcaaaaga gtgttttgat aaatacggtg tagatgcggt tatggtagga
     1441 cgtgcgtgca ttggcaaacc ttggattttt caagaaataa gacactatct acaaacaggc
     1501 gaacatatac cacaaatccc atttactgaa caacttagca tactcaaaga ccaggtagag
     1561 cgaagcatcg aatggctgga cgaacgtaga ggtattttac atatccgtag gcatttagct
     1621 gccacaccct tattcaaagg aatcccaaac tttaaagaca cccgcattgc tatgttgcgt
     1681 gccacaacaa aagacgagtt atttgccatt tttaattcta tagaaactat ggtggtggag
     1741 agatagctta ctcctttacg gtgtatcaaa aaagcttatc ctcaaacttt atgttttttt
     1801 ttgtaccttt gcaccgattt ccaatttgca taacgcattt tttataccta tgaatacctt
     1861 tgaccaactc gaactgacca ccccattgcg taacgctatt gctgatatgg ggttaattga
     1921 acctacacag atacaaaccg aagctttttc ggtaa
//
LOCUS       DAOPUR010000304         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_63506_length_1953_cov_3.592729, whole genome shotgun sequence.
ACCESSION   DAOPUR010000304 DAOPUR010000000
VERSION     DAOPUR010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63506_length_1953_cov_3.592729"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKN90_05750"
     CDS             <1..371
                     /locus_tag="PKN90_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNZ85813.1"
                     /translation="YRFTSAVSGNLSVDENERVEVVDNQQYKDSCIQKVNTLTTLNGV
                     QQLPERFYIVIGSFQTERMAQRFVSELPAIFSSATIVFSENRYRITTASFETEQEGEY
                     FLHTFQQNHPRFKDAWLLKK"
     gene            489..1019
                     /locus_tag="PKN90_05755"
     CDS             489..1019
                     /locus_tag="PKN90_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNZ85814.1"
                     /translation="MATKIRLQRHGRKGYAYYHIVVADSRAPRDGKFIERIGSYNPNT
                     NPATVNVNFEKALKWIETGAQPTDTVRNILSREGVMMKKHLLGGVKKGAFTAEEADKK
                     FDAWMKNKQVAVAKVKEKLASDASMTAKQRLDAEKAANKAKAAEVAKKQAEKLAVAEP
                     VAEEVATTETETSVAE"
     gene            1086..>1953
                     /locus_tag="PKN90_05760"
     CDS             1086..>1953
                     /locus_tag="PKN90_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNZ85815.1"
                     /translation="MVLEKNCRVLPKIAATENLLKEYLAKELDITPKSIHAIRILKRS
                     IDARQRMVFVNLQIRIFIDEKPKEERVFLPTYKDVSQAKKQVIVVGAGPGGLFAALRL
                     IELGIKPIVVERGKDISKRKKDIALISTSHKVDADSNYCFGEGGAGAFSDGKLYTRSK
                     KRGNVDKILQVLCYHGASHSILIDAHPHIGTDKLPSVIKNMRETILSYGGEVYFETRM
                     DDLLIQHNQVKGVVTNTGQTFLGNVLLATGHSARDVYRLLQHKEVAIEAKGIAVGVRL
                     EHPQALIDQLQYH"
BASE COUNT          712 a          298 c          411 g          532 t
ORIGIN      
        1 gataccggtt tacctcagca gtgtctggaa atttgtctgt ggatgaaaat gagagagttg
       61 aagttgttga taatcagcaa tataaagaca gttgtattca aaaagtgaat acgctgacta
      121 ctttgaatgg tgtacaacaa ctaccagaac gtttttatat cgttataggg agttttcaaa
      181 cagaacgaat ggctcaacga tttgtgtcag aattgcctgc catattcagt tctgctacta
      241 ttgtgttttc tgaaaaccga tatcgtataa ccacagcaag ttttgaaaca gagcaagagg
      301 gagaatattt tcttcacact tttcagcaaa atcatccgcg atttaaagat gcttggttgc
      361 ttaaaaaata aattttccct aaaaaaactg ccgataagta tttgtcagct taataattag
      421 tagtatcttt gcatccgcat tcgggaaatg cacagaagtt tattaactaa taaaaaaaac
      481 aaaaaagtat ggcaactaaa attagattgc aaagacatgg acgcaaaggg tatgcttatt
      541 accacattgt agtcgcagac agcagggcac cacgtgatgg taagtttatt gaaaggatag
      601 gttcgtataa cccgaacaca aatcctgcta cagtaaatgt aaacttcgaa aaagccttaa
      661 aatggattga aactggtgct caaccaacag acactgtaag aaatatttta tccagagaag
      721 gtgtaatgat gaaaaaacac ttattgggtg gtgtaaaaaa aggtgcattt acagcagaag
      781 aagcagataa gaagtttgat gcttggatga aaaataaaca agtagctgta gcaaaagtga
      841 aagaaaaatt agctagtgat gcttcaatga cagcaaaaca acgtttggat gcagaaaagg
      901 cagcaaataa agcaaaagca gctgaagtag caaaaaaaca agcagaaaaa ttggctgtag
      961 ctgagcctgt agccgaagaa gtagcaacta cagaaacaga aacctctgtt gcagagtaat
     1021 taaagtaata atatgaaaaa gagctgcccg tattgtatgg cagctctttt tttatctaaa
     1081 taagtatggt actagaaaaa aactgtaggg tattacccaa aatagctgct actgagaatt
     1141 tattaaaaga atacctagca aaagaactag atattacgcc aaagagtata catgcaatac
     1201 gtatcctaaa acgttctatt gatgctcgtc agcggatggt ttttgttaat ttgcagatac
     1261 gtatctttat agatgaaaaa cccaaagaag agcgtgtatt tttgcctact tataaagatg
     1321 tatcccaagc aaaaaagcaa gtcattgtag tgggagctgg acctggtggg ttgtttgccg
     1381 ctttgcgatt aattgaatta ggaattaaac cgattgtggt ggaaagaggc aaagatatat
     1441 ctaaacgcaa gaaggatata gcattaattt ctacatccca caaagtagat gctgattcta
     1501 actactgttt tggagaaggt ggagcaggag ctttttcaga cggaaaactg tatactagga
     1561 gtaaaaaaag ggggaatgta gataaaatat tgcaggtgtt atgttatcat ggagcatcac
     1621 actctatttt gattgatgcc catccccata ttggtacaga taaattgcct agtgttataa
     1681 aaaatatgag agaaacaatt ctttcgtacg gaggagaagt ttattttgaa acgcgtatgg
     1741 atgatttgct tatacaacac aatcaagtaa aaggagttgt aacgaataca ggtcaaacat
     1801 ttttaggaaa tgtactatta gcgacagggc attctgctcg agatgtgtat cggttgctac
     1861 aacacaaaga agttgctata gaagccaaag gaattgctgt tggtgttcgt ttagagcatc
     1921 cacaagcatt gatagaccaa ttacaatatc ata
//
LOCUS       DAOPUR010000305         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_63513_length_1953_cov_3.245522, whole genome shotgun sequence.
ACCESSION   DAOPUR010000305 DAOPUR010000000
VERSION     DAOPUR010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63513_length_1953_cov_3.245522"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..794
                     /gene="ruvA"
                     /locus_tag="PKN90_05765"
     CDS             207..794
                     /gene="ruvA"
                     /locus_tag="PKN90_05765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNZ85816.1"
                     /translation="MIEYIKGDVVELNPSFVVIETNGIGYFIHISLTTYAHLQQTTAC
                     QLYVYESIREDAHVLYGFSKKEERQIFLHLISVSGVGANTARVILSSLSALEIQEAIA
                     TANENVLKAVKGIGLKTAQRIIVDLKDKLGNMELATKNVVTLQVNTSMKKEAVSALVM
                     LGFPASASQKVVDKILADNADMKIEDVIKKALKML"
     gene            815..>1953
                     /gene="sprA"
                     /locus_tag="PKN90_05770"
     CDS             815..>1953
                     /gene="sprA"
                     /locus_tag="PKN90_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNZ85817.1"
                     /translation="MTKIHKILVFISLCGLIFYSSLVANDTIDTSVSLAKKETEPSDE
                     SLRYPISQSLENAPLTQQTNAVIDLPSPQNIASSIEYDPLTEQFIFSKKLNDQDLITP
                     TYLTPEEYSKYQMQQSVNAYWRTRNRQEKDNEKPFSLTDIQIGLGDADKIFGPGGVQL
                     KLQGSAELLFGLKFYRSKNPSLSERLQSPPPVFDFDQKIQLNVNGSVGDKMSFGLNYN
                     TEATFDFDQSMLKLRYEGKEDDIIRKIEAGNVSMPLSGSLITGNTTLFGFKTEMQFGK
                     LNVTTVLSQQESESKTINLSGGAQTTDFDIAADQYDENRHFFLAHYFRDNYDSFMSKL
                     PHITSGIVINKIEVWITNKNAVDFNQARNIVGFMDLAEPTRINNT"
BASE COUNT          652 a          319 c          361 g          621 t
ORIGIN      
        1 agtttttact aatcattgtt gggatctacc ttttttatac gttaaaaaag attattttat
       61 atacgttttt tgccttttct tcgttctagc tacacacgtt ttatatagtg tataagatag
      121 catgtgatgg aaaattttga aaagactatt ttttttatgt atctttgaac ccttttttag
      181 agccatccaa tattagataa gtatttatga ttgaatatat taagggagat gttgtagagc
      241 taaatcccag ttttgtagta atagaaacca atggaatagg gtattttatc catatttcac
      301 ttactaccta tgcccattta caacaaacga cagcttgtca attgtatgtt tatgaatcca
      361 ttcgcgaaga tgcacatgtg ttgtatgggt tctcaaaaaa agaagaaaga caaatctttt
      421 tacatcttat ctctgtgtcg ggagtaggag ccaatactgc tcgggttatt ttatcatctc
      481 tttcggcatt agaaatacaa gaagccattg caacagccaa tgaaaatgtg ttgaaggctg
      541 taaaaggaat tgggttaaaa acggcacaac gtatcattgt tgatttgaag gacaaactag
      601 gcaatatgga gttagctact aaaaatgtag ttactctgca agttaatacc tcaatgaaaa
      661 aagaggcggt atctgcattg gtgatgttag gttttccagc ctctgcttca caaaaagtgg
      721 tggataaaat tttggcagat aacgcagata tgaaaattga agacgttatc aaaaaagcat
      781 taaaaatgtt gtaacaataa agcgttcgtt taccttgact aagatacata aaatactcgt
      841 ttttataagc ctttgtgggt tgattttcta ctcttcgttg gtggctaacg atactattga
      901 tacctctgta agtttagcaa aaaaagaaac agaaccatct gatgaatcgt tacgttatcc
      961 tatttcgcaa tcgttggaaa atgccccttt aacccaacag acgaatgccg taatagactt
     1021 accatctcca caaaatatag catcttctat cgaatatgac ccgttgactg aacagtttat
     1081 tttttctaag aaactgaacg atcaagattt aataacgcca acatatctaa cgccagaaga
     1141 gtactctaag taccaaatgc aacaatctgt taatgcctat tggcgtacac gcaatagaca
     1201 ggaaaaagac aatgaaaaac cattttctct taccgatatt caaattgggt tgggcgatgc
     1261 tgacaaaata tttggaccag gaggggtgca gttgaagctt caaggttcgg cagaattatt
     1321 gttcggactt aaattctata ggtctaaaaa tccatcttta agtgaacgat tacagagtcc
     1381 acctcctgta tttgattttg atcaaaaaat acaactgaat gtgaatggtt ctgttggaga
     1441 caaaatgtcc ttcggattaa attacaatac tgaagccact tttgatttcg accaatcaat
     1501 gctcaaactg cgttacgaag gcaaagaaga cgatattata cgcaaaattg aagcaggtaa
     1561 cgtaagtatg ccattgagtg gaagtttgat tactggtaat actactttgt ttgggtttaa
     1621 aacggaaatg caatttggta agttaaatgt aactaccgtt ttatcgcaac aagaatcaga
     1681 atcaaaaact attaatttaa gcggaggagc acagacgaca gattttgata ttgccgctga
     1741 tcagtatgat gaaaataggc attttttcct tgcacactat tttcgcgata attatgattc
     1801 ttttatgagc aaattgccac atatcacctc tggtatagtg attaataaaa ttgaagtgtg
     1861 gattaccaac aaaaatgcgg ttgattttaa tcaggctcgt aatattgttg gttttatgga
     1921 tttagctgaa ccaacacgca tcaataatac tca
//
LOCUS       DAOPUR010000306         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_63816_length_1947_cov_2.849366, whole genome shotgun sequence.
ACCESSION   DAOPUR010000306 DAOPUR010000000
VERSION     DAOPUR010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63816_length_1947_cov_2.849366"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PKN90_05775"
     CDS             complement(<1..1221)
                     /locus_tag="PKN90_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNZ85818.1"
                     /translation="MQHTYADFGEYTITLSIMPTNNAQCVYQQEVSIVLDDCFLEGDI
                     NVYQIACNQFAINSFIQNNDKEYSLQWYVDNVPYDANDISPLTLPAGQHDIRLEVVDN
                     AEPTCTLTLTKEISVYEPTINIHTVVNPQTLEMTFNAVVQGIDEFDFYWYFGDSGNGS
                     GDDTVLSGTYTYPSAGTYDIAFSLQPKGSMGGCSYHKNIQVVIPEVQQNDPCDKASIK
                     GKLHADIAMLIANAVQVDAYKKNSNDKYELLKSASISQSGDFEFADLLEGDYLLVGRI
                     INPEQYPLVIASYFNQMMEQVFSWQEASPIQLVCNATAHVELSMITLEDLLTGGASIS
                     GYVYYEGGTRNLIVSALKSKRFSLFAGGMNVYLYNADTNVLVAQTVTDANGYYSFSGV
                     PTGNYTVMVDMPGVP"
     gene            complement(1112..>1947)
                     /locus_tag="PKN90_05780"
     CDS             complement(1112..>1947)
                     /locus_tag="PKN90_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85819.1"
                     /translation="VAAEYETSSSIHDGGLWLHPAVATFDFTEMQGEIYSISLKVADW
                     CPPSGCTTIRFYANNEVVQSYFTNTTSIDHIIVNRSEQGMSIDSMTVLSFEGVIKYVK
                     IQKEEIEQTCTLQAQINLEPIDCTTFGISTSIQNNQKSYIEEWFLNDIPQTPQEYLEF
                     ELNEPGVYTLKLVVTDQEDASCTVTEEKEIVIATPPTITITPTLNKITKEVSLKGEVD
                     GIEGYTFAWNFGDATPLNTTDLSYATHVCRLWRVYDNLVYNAHEQCTMCLSTRSIHCF
                     R"
BASE COUNT          574 a          403 c          312 g          658 t
ORIGIN      
        1 cggtacccct ggcatatcaa ccataaccgt ataattacct gttgggacgc ccgagaagct
       61 ataatagcca tttgcatctg taacagtttg tgcaactaac acattggtgt ctgcattgta
      121 caaatacaca ttcatgccac cagcaaacaa ggaaaatctc ttacttttaa gagcactaac
      181 aattaaattt cgtgtaccac cttcatagta cacataccct gaaatggatg ctccgccagt
      241 taacaagtct tctaatgtta tcatacttaa ctccacgtga gcggtagcgt tacatactaa
      301 ttgtatagga gatgcttctt gccaagaaaa cacctgctcc atcatttgat tgaaatagct
      361 tgctattact aatggatatt gttcggggtt aataattcta cctactaaca aataatcacc
      421 ttctaacaaa tcagcaaact caaaatcgcc cgactgacta atagatgcac ttttaagtag
      481 ctcatattta tcattgctgt ttttcttata cgcatctact tgtactgcat tggctatcaa
      541 catagcaata tctgcatgca atttaccttt tattgaggct ttatcacatg ggtcgttttg
      601 ttgcacctca ggtatcacca cttgtatgtt tttatgataa ctacatccac ccatactccc
      661 ttttggctgt aatgagaacg caatatcata tgttcccgct gatggatagg tatacgtacc
      721 acttaataca gtatcatctc cacttccgtt gccactatct ccaaagtacc aataaaaatc
      781 gaactcatct atcccctgta ccactgcgtt aaaagtcatt tccagtgttt gaggattgac
      841 tacagtatga atattgatgg taggttcata tactgatatc tccttggtaa gtgtaagtgt
      901 acaagtcggc tctgcattat ccactacctc taacctaatg tcatgttgtc ccgctggaag
      961 ggttaatgga gaaatatcat tggcatcgta tggcacatta tctacatacc attgcaatga
     1021 atattcttta tcattatttt gaataaagga atttatggca aattgattgc acgcaatttg
     1081 gtacacatta atatctcctt ccaagaaaca atcatctaaa acaatggata cttcttgttg
     1141 ataaacacat tgtgcattgt tcgtgggcat tatagacaag gttatcgtat actctccaaa
     1201 gtcggcatac gtgtgttgca tacgataaat cagttgtatt cagaggagtg gcatctccaa
     1261 aattccatgc aaaagtatag ccctctatgc catctacttc tcctttcaag cttacttctt
     1321 tcgttatctt atttagagta ggagtaattg tgatagtagg aggtgtagca atcactatct
     1381 ctttttcttc agttactgtg caagaagcat cttcttggtc tgtaactact aattttaatg
     1441 tatatacccc tggttcattt agttcaaatt ctagatattc ttgaggtgtt tggggtatat
     1501 catttaaaaa ccattcttca atatatgatt tttgattatt ttgtatgcta gtacttatcc
     1561 caaacgtagt acaatcaatt ggttctagat ttatctgtgc ttgcaatgtg catgtttgtt
     1621 ctatttcttc tttttgaatt tttacatact taattacacc ttcgaacgat aaaactgtca
     1681 ttgaatctat tgacatacct tgttctgaac gatttacaat gatatgatca atacttgtag
     1741 tgttggtgaa ataactctgt accacttcat tgttagcata aaaacgtata gtagtacaac
     1801 cagatggagg gcaccaatct gccactttta aactaatgct ataaatttcg ccttgcattt
     1861 cggtaaaatc aaatgtagcc acagcaggat gcaaccacaa tccaccatca tgaatactcg
     1921 atgatgtttc atactctgct gctactc
//
LOCUS       DAOPUR010000307         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_64010_length_1943_cov_3.424258, whole genome shotgun sequence.
ACCESSION   DAOPUR010000307 DAOPUR010000000
VERSION     DAOPUR010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64010_length_1943_cov_3.424258"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..234)
                     /locus_tag="PKN90_05785"
     tRNA            complement(162..234)
                     /locus_tag="PKN90_05785"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Met,seq:cat)
     gene            484..1341
                     /locus_tag="PKN90_05790"
     CDS             484..1341
                     /locus_tag="PKN90_05790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ85820.1"
                     /translation="MKKKIGNFFKKIRIKYKLSVLNENTLEEEYRFRLSALSLFTLFM
                     GLTLITFTLFSILILTTPLKRLLPDNTDRKVRNEVVLSALRVDSLADELNKSQQYLAV
                     IREIVAGDVPIDTTHSTESLISSRIDEKFIEKSDAEKAFAEQYEQEEKYNIINPLTQQ
                     QNTNLVFFTPVKGIIREPFQPRINNHFGISIVVNQSKSVSSIYKGTVILTGYDVNNGY
                     FIQVLHPQNFISIYKQATVILKKEGDLVKTGEVIALVDAQGNKPPVLQFELWYEGRAQ
                     DPQDYIIFE"
     gene            1357..>1943
                     /locus_tag="PKN90_05795"
     CDS             1357..>1943
                     /locus_tag="PKN90_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNZ85821.1"
                     /translation="MNKKQIAILGSTGSIGTQALEVIAAHPDKFEVYAITANNSVDLL
                     IEQAVRFQPEMVIIANEQHYAYIKKALKNYPIKVYAGVKAICEMVELPSVDVVLTAMV
                     GYAGLLPTIHAVKAGKRIALANKETLVVAGEIICDLAQQYRSAIIPVDSEHSAIFQCL
                     IGEQNASVEKIILTASGGPFRQKTYDELRQVTTRDA"
BASE COUNT          618 a          314 c          404 g          607 t
ORIGIN      
        1 gcaaagttaa aaaagatttc caaatacgga ttacatgaga gttagaaagt acaaagctag
       61 aaagttgaaa gtgtaaaatg gagccgtaag cttttagtcg ttgctatcca atcagccaaa
      121 agcaaaaaag agaagaaacc tgtgtttctt ctcttcctga gttgcgggga ttcgactcga
      181 acgaatgacc tttgggttat gagcccaacg agctaccaac tgctccaccc cgcgatatat
      241 tttttagcta taaagctgta ttctccaatt ggactacaaa gatagtgcgt tcatttgtgt
      301 tatccaaatt atttgggata tatttttgtt taaaattgtt ttgttggaaa aaggtatcct
      361 aatttttgct aaaagttctc tttcatgcgt gttttatgtt ttttacatgt tttatgttta
      421 aaaaaatgct atttttgtgt ggtaaaaaat ggagtgtcag tatccatatt tttgtgcatg
      481 ccgatgaaaa aaaaaatagg gaattttttt aaaaaaatac ggattaagta taaactttct
      541 gttcttaatg agaatacatt agaggaggag taccgtttta ggttgtctgc attgagctta
      601 tttacccttt ttatggggct tacattgata acgtttaccc tgttttctat ccttattctt
      661 actactccgc ttaaacgttt gttacctgat aataccgata gaaaagtgcg taacgaggtt
      721 gtgttgagtg ctttgagggt cgattcgttg gctgatgagc ttaataaaag ccagcaatat
      781 ttagccgtta tcagagagat agtggcaggc gatgtgccta ttgatacaac acatagcact
      841 gagtcgttga tatcaagtag gatagatgaa aaatttattg aaaaatcgga tgccgaaaaa
      901 gcctttgctg agcaatacga acaggaagaa aaatacaata ttattaatcc gttgacacaa
      961 caacaaaata caaatttggt gttttttact cctgtaaaag gcattattag ggagccattt
     1021 caacctcgta taaataatca ttttggtatt tccattgtag taaatcaaag caaaagtgtc
     1081 tcttctattt acaaggggac ggttatcctg actggttatg atgtaaataa tggttatttt
     1141 atacaggttt tgcatcctca aaattttata tctatctaca aacaggcaac cgtaattctg
     1201 aaaaaagaag gggatttagt taaaacggga gaagtgattg cattagtcga tgctcaaggc
     1261 aataaacctc cagtgttgca atttgaattg tggtatgagg gaagggctca ggatccgcaa
     1321 gattatatca tttttgaata aactgttgac aaccgaatga ataaaaaaca aatagcgata
     1381 cttggttcta ctggctctat aggtacgcaa gctttagagg ttattgctgc tcatccagat
     1441 aagtttgagg tatatgccat tacggcaaat aatagtgttg atttgctaat tgagcaagca
     1501 gttcgttttc aacccgaaat ggtgattatt gccaatgaac agcattatgc atatataaaa
     1561 aaagccttaa aaaattatcc gataaaggtg tatgccggag taaaagctat ttgcgaaatg
     1621 gtagagttac cgtcggttga tgtggtactt acagcgatgg taggatacgc gggcttattg
     1681 cctacaattc atgctgtaaa agcgggcaaa cgtatagctt tagccaacaa agaaaccttg
     1741 gtggtagctg gcgagattat ctgcgatttg gcacaacaat accgttcggc aattatccca
     1801 gtcgattcgg agcactcagc catttttcaa tgtttgattg gcgaacaaaa tgcttctgtc
     1861 gaaaaaataa tactgaccgc ttcgggtggt ccttttcgcc aaaaaacgta cgatgaattg
     1921 cgtcaagtaa caacccgtga tgc
//
LOCUS       DAOPUR010000308         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_64066_length_1942_cov_3.470058, whole genome shotgun sequence.
ACCESSION   DAOPUR010000308 DAOPUR010000000
VERSION     DAOPUR010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64066_length_1942_cov_3.470058"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PKN90_05800"
     CDS             <1..1466
                     /locus_tag="PKN90_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HNZ85822.1"
                     /translation="TRNVIASIRYAKEAGMIAQAALSLTVSPVHTVDYYCAMADELIA
                     NGADEICLKDMAGIGRPVSLGKIVAYIKKKYPTIIVQYHGQTGPGFTPASILEVCKAG
                     CDIIDVGMEPLSWGTGHADVIMVREMLKDAGFSVPEINMSAYMKVRALTQEFMDDFLG
                     YFIPETNRHLQSLLIAPGLPGGMMGSLMNDLKDNLVSINKAYQKQGKTTINTDELLIQ
                     LFDEVAYVWPRVGYPPLVTPYSQYVKNLAMFNIIQMAKGKARWTMISDDTWDMILGKA
                     GRLPGELAPEIKELATQQGRTFYEGDPQILYPNVLENFRKEIIDNGWDMGKDEEELLE
                     LAMHPEQYRTYKSGKAKHDFEIELAQAKKESQQRGDGSFVLPHVLEMTVNGEKYKVEL
                     AEFANSGLDSNVLLKENAEGEKVLAPLEGRFFLTKSASDIPLKIGSQVKKGDIVCYVE
                     AMKTYNAIQAETDGVIVDFVVANGDNVSEDDVLLIIQ"
     gene            1479..>1942
                     /locus_tag="PKN90_05805"
     CDS             1479..>1942
                     /locus_tag="PKN90_05805"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ85823.1"
                     /translation="MEILGNLYEMTAFSNIIAQPQFLLMYAIAFLLLYLGIKKEFEPL
                     LLIPIAFGVLIANFPGGQMGIIQANSEGLVEVGGEMKSIWQMPLHDIAHEMGIMNFLY
                     YMLIKSGFLPPIIFMGVGALTDFGPMLRNIRLSIFGAAAQLGIFTVLIGAILL"
BASE COUNT          612 a          258 c          449 g          623 t
ORIGIN      
        1 atacccgaaa tgttatagca tctattcgat atgccaaaga agcaggtatg atagcacaag
       61 ctgcattgtc gcttactgtg tctcctgtgc atacggttga ttattattgt gcaatggcag
      121 atgaacttat agctaatggt gccgatgaaa tttgtttaaa agatatggca ggcattggtc
      181 gccctgtatc attaggaaaa atagtagcat atataaagaa aaaataccca actattattg
      241 tgcaatatca tggacagact ggtcctggtt ttactccggc ttctattttg gaggtttgta
      301 aagctggttg tgatattatt gacgttggga tggagccttt atcgtggggt actggtcatg
      361 cagatgtaat tatggtgcga gaaatgttga aagatgctgg ttttagtgtg ccagaaatca
      421 atatgtcagc ttatatgaaa gtgcgtgcat tgactcaaga atttatggat gattttttag
      481 gatattttat tcccgaaacg aatagacatt tgcaatcgct gttgatagca cctggattgc
      541 caggcggtat gatgggtagt ttgatgaatg atttgaaaga taatttagtg tcaataaaca
      601 aagcatatca aaagcaaggt aaaactacca ttaatacaga tgaattgttg attcaactat
      661 ttgatgaagt ggcttatgtg tggcctcgcg ttggctatcc tcctttggta acgccatatt
      721 ctcagtatgt taaaaattta gcgatgttta atatcattca aatggctaaa gggaaagctc
      781 gttggacaat gatatctgat gatacttggg atatgatact tggtaaagct ggacgattgc
      841 caggagaatt ggctcctgag attaaagagt tagctacaca gcaaggtcgt acattttatg
      901 aaggagaccc tcaaattttg tatccgaatg tgttagaaaa ttttcgaaag gaaataattg
      961 ataatggatg ggatatggga aaagatgagg aagaattgtt agaacttgcc atgcatccag
     1021 aacaatatag aacgtataaa tcgggtaaag ctaaacatga ttttgaaata gagttagcac
     1081 aagcaaaaaa agaatcgcaa caacgaggag atggttcttt cgtattgcca catgttcttg
     1141 aaatgactgt aaatggagaa aaatataaag tagaacttgc agaatttgcg aatagtggat
     1201 tagattcgaa tgttcttcta aaagaaaatg cagaaggaga aaaggtgttg gctcctttag
     1261 aagggaggtt ttttttgaca aaatcagcct cagatattcc cttgaaaatt ggatcacaag
     1321 ttaagaaagg ggatattgtt tgttatgtag aggcaatgaa aacatataat gctattcaag
     1381 ctgaaactga tggtgtaatt gttgactttg tagttgctaa tggagataat gtttcagagg
     1441 acgatgtgtt gcttattatt caataatctt aatgatatat ggaaatttta ggaaatttgt
     1501 atgaaatgac agcgtttagt aatattattg ctcaaccgca atttttactc atgtatgcca
     1561 ttgcattttt attgttgtat ttaggtataa aaaaggaatt tgagccatta ttgttgattc
     1621 cgatagcctt tggggtatta atagccaatt tcccaggtgg gcaaatgggg attatacagg
     1681 ctaactcaga aggattggta gaagtgggtg gtgaaatgaa aagtatttgg caaatgccgt
     1741 tgcatgatat tgcacacgag atgggaatta tgaatttttt atactatatg cttattaaat
     1801 cagggttcct tcctccaata atttttatgg gagtaggagc tttaacggat tttggtccga
     1861 tgttgcgaaa tatccgatta tccatttttg gagctgctgc acaattgggt atttttacgg
     1921 tactgatagg ggctattttg ct
//
LOCUS       DAOPUR010000309         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_64230_length_1939_cov_2.290870, whole genome shotgun sequence.
ACCESSION   DAOPUR010000309 DAOPUR010000000
VERSION     DAOPUR010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64230_length_1939_cov_2.290870"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKN90_05810"
     CDS             <1..724
                     /locus_tag="PKN90_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ85824.1"
                     /translation="EMHYLDIVDCNAGDHGKAFATNFNPEINIREVSQRGKYWENGEW
                     VETEPHEIHKPLNYPEIGPKESYVIYHEELESLVKNFPTLKRARFWMTFGQEYLTHLR
                     VIQNIGMARIDEVEYNGQKIVPIQFLKAVLPNPGDLGENYTGWTSIGCRIKGIKNGEE
                     KTYYIYNNCSHEEAYKETGTQGVSYTTGVPATVGAMMFLTGEWGGAGVFNVEEFNPDP
                     FLHELAQQGLPWHELHNIDLEL"
     gene            1006..>1939
                     /locus_tag="PKN90_05815"
     CDS             1006..>1939
                     /locus_tag="PKN90_05815"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HNZ85825.1"
                     /translation="MKTSIHLTLILLLFFGSFHLTAGTYSGGTGTENDPYQIATYADL
                     LELSATSADWATGKYFIQTAHIDASATATLNGGKGFVPIGNLDYKFQGSYDGQHYTIS
                     NLYINRSDEDRIAMFGYLWGAGISVQNLGLTNVSITGKNSVAALAGYNRDGIINNCYS
                     SGSITGYSSIGGLVATNYMATITNSYSTCSVSGTGTMGGCVGVNNQSVISRCYATGSV
                     TGLSVLGGFVGSNKASDTKAATISHSYSTGNVVRTAAPNVKIGGFCGTNELKGAIQNC
                     YSTGSVNFGTAQGFLGENITNATFSGNFYDSQTSK"
BASE COUNT          621 a          318 c          417 g          583 t
ORIGIN      
        1 tgagatgcat tatttagata ttgtcgattg caatgctggc gaccatggca aagcatttgc
       61 taccaatttt aaccccgaaa tcaatatccg tgaagtgagc caacgaggca aatattggga
      121 aaatggcgaa tgggtagaaa cagaaccaca cgaaattcat aaaccactga attatccaga
      181 aattggtcca aaagaatctt acgttatcta ccacgaagag ttggagtcgc tggtaaagaa
      241 ttttccgacc ctgaaaagag cccgtttttg gatgacattt ggacaagaat atttaactca
      301 cttacgtgtg attcagaata ttggtatggc tcgcatcgat gaggtggaat acaatggaca
      361 gaaaattgtg ccaattcaat ttttaaaagc tgtattgcct aatccaggcg atttgggcga
      421 aaattataca ggatggacat ctatcggttg tcgcattaag ggtattaaaa atggagaaga
      481 aaaaacgtat tacatctata ataattgtag ccacgaagag gcatacaaag aaacaggcac
      541 acaaggggta agttatacca caggtgtacc agccacagta ggtgctatga tgtttttaac
      601 tggcgaatgg ggtggagcag gtgtatttaa tgtagaagag tttaatccag atccattttt
      661 gcacgaatta gcacagcaag gcttgccttg gcacgaattg cataacattg atttagaatt
      721 ataacgccaa taagggtcta cgttttatta aacagcacga taaattacta tgtgctgttt
      781 ttttttgctc tggagtgttt gttattcgtg ttggttgcaa tatagttaat attcgataaa
      841 aatttgggta atctgttgta aatataaata attagataga tttttgttat aaattttata
      901 aaaccagtat gtggtaggtc gtctacttaa tggtatatat gacgtatgta atttcataaa
      961 agccataaga ccacttacat tatgaacaag acaataaaaa actatatgaa aacatcaata
     1021 caccttacgt tgattctact tctatttttc ggaagttttc atcttactgc cggaacttat
     1081 tctggaggca ctggcacaga aaatgacccc taccaaattg ctacatatgc cgatttactt
     1141 gagctatctg ccacctctgc cgattgggca acaggcaaat attttataca aactgcacat
     1201 attgatgcca gtgctactgc tacattgaat gggggtaagg gttttgtacc tattggaaat
     1261 ttagactata aatttcaggg gagctacgat ggtcaacact ataccatatc taatttgtat
     1321 atcaaccgaa gtgatgaaga tagaattgcc atgtttggtt atctatgggg agcgggtatt
     1381 agcgttcaaa atctgggtct aaccaatgtt tctataacag ggaaaaactc cgtggcagct
     1441 ttagcgggat ataatcgtga tggcattata aacaactgct attcttcagg tagtataact
     1501 ggttattctt ctataggagg attagtggca acaaactaca tggctactat aacaaatagt
     1561 tattctacat gcagtgtcag tggcacgggt acaatgggtg gatgtgtagg tgtaaataat
     1621 caaagtgtta tttcacgttg ttatgcaaca ggtagtgtta ctggattaag tgtgcttggc
     1681 ggatttgttg gtagtaacaa agcaagtgat accaaggcgg caaccattag tcattcgtat
     1741 agtacaggca atgtggttcg aactgctgca cccaatgtaa aaattggtgg attttgtggt
     1801 actaacgaac tgaaaggagc tattcagaat tgctattcta ctggctcggt aaattttggc
     1861 acagctcaag gctttttggg tgagaatata accaatgcaa ctttttcagg taatttttac
     1921 gattcacaaa caagcaaac
//
LOCUS       DAOPUR010000310         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_64877_length_1926_cov_3.922501, whole genome shotgun sequence.
ACCESSION   DAOPUR010000310 DAOPUR010000000
VERSION     DAOPUR010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64877_length_1926_cov_3.922501"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1027)
                     /locus_tag="PKN90_05820"
     CDS             complement(80..1027)
                     /locus_tag="PKN90_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNZ85826.1"
                     /translation="MKKHLLCLIALFYFFISTTYSQENTLPRVSSGTIIRHVDFPSNY
                     ISARNVDVWLPDNYSPNQRYKVLYMHDGQMLFDANTTWNKQEWGVDETITRLIANDSI
                     LSTIVVGIWNSGRTRHSDYFPQKPFERLPVSTQDSLILLGRRQNNVSLFAERVQSDNY
                     LRFIVEEVKPFIDKTYATLTEPEHTFIAGSSMGGLISMYAVCEYPEVFGGAACLSTHW
                     IGSFQTEGNPIPAQFFAYLKENLPSPDTHKFYFDFGTETLDVHYEPYQQQVDIIMEEK
                     GYTSTNWITRKFEGKDHSENAWRERLHIPLTFLLPIQFN"
     gene            complement(1073..1450)
                     /gene="folK"
                     /locus_tag="PKN90_05825"
     CDS             complement(1073..1450)
                     /gene="folK"
                     /locus_tag="PKN90_05825"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNZ85827.1"
                     /translation="MNTHHYILLLGSNFLAHKHLSIAIELLQQEFGIHRTSHEMETKA
                     IDMPNDYPFVNKAVCIQTRLEPTELIQKIKEMEKQLGRSDEKTLQGIIPIDIDVILCD
                     KKVLHSDYETKNYVKKLVEEVSV"
     gene            complement(1453..>1926)
                     /locus_tag="PKN90_05830"
     CDS             complement(1453..>1926)
                     /locus_tag="PKN90_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ85828.1"
                     /translation="FAVQLDSDDLYENATTLQQIVNAFYEQNCAMVIGSYSMTDFALQ
                     PIPPGLIDHKEWTPENGRNNALRINGLGAPRAFYTPVLREIKVPNTSYGEDYALGLQI
                     SRNYQIGRIYNSVYLCRRWNENSDAALDIEKMNAHNAYKDKLRSIEMMARIAMNK"
BASE COUNT          549 a          348 c          366 g          663 t
ORIGIN      
        1 tgttgcgagt actctgaact agaagtgcaa caagatgaat tattttctgt tttcataaaa
       61 ttgttttttt tgttgataat taattgaatt gtatgggtaa taaaaacgta agcggaatgt
      121 gtaagcgttc acgccacgca ttttctgaat gatcttttcc ttcaaatttt cgtgttatcc
      181 aattggtaga ggtgtatcct ttttcctcca tgatgatatc tacttgttgt tggtacggtt
      241 cgtaatgaac atctaatgtt tcggttccaa aatcgaagta aaatttgtgt gtatctggtg
      301 agggtaaatt ttcttttaaa taggcaaaaa attgtgcagg aattgggttc ccttctgttt
      361 gaaaactacc aatccaatgt gtagataaac aagctgcacc accaaaaact tctgggtatt
      421 cgcatacagc atacatcgaa ataagtccgc ccatgctaga gcctgcaata aatgtatgtt
      481 caggttcggt aagcgtagca tacgttttgt caataaaagg ttttacttcc tctacaataa
      541 aacgtaggta attatccgat tgcacacgtt cggcaaataa tgacacatta ttttgtcttc
      601 tacccaataa aattaatgag tcttgtgtag atacaggcaa gcgttcaaaa ggtttttgcg
      661 gaaaataatc cgaatgacgc gttcgtccag aattccatat tcctactaca atggtactca
      721 gaatactgtc gtttgctata aggcgggtaa tggtttcgtc cacaccccat tcttgtttgt
      781 tccaggtagt attggcatca aaaagcattt gtccatcgtg catatagagc actttgtaac
      841 gttggtttgg gctgtaattg tcaggtagcc aaacatctac attgcgagca gatatatagt
      901 tagaaggaaa atctacatga cgtataatgg taccactgct tacacggggc aaggtgtttt
      961 cttggctgta cgttgtacta ataaagaaat aaaaaagagc gataaggcaa agtaagtgtt
     1021 ttttcatgcg tatatataat ttgttctgaa aacaaatata cgtacaattt tctcaaacgc
     1081 tcacttcttc taccaatttc ttaacataat tttttgtttc ataatccgaa tgtaacactt
     1141 ttttatcaca gagaatcaca tcaatatcaa tgggaataat gccttgcaag gttttttcat
     1201 ccgaacgacc aagttgcttt tccatttctt ttattttctg tattagctct gttggctcaa
     1261 gcctagtttg tatgcaaaca gccttattta caaaaggata gtcgtttggc atatcaatgg
     1321 ctttcgtttc catttcgtgc gaggtgcgat ggataccaaa ttcttgttgc agcaattcaa
     1381 tggctattga cagatgtttg tgtgccaaaa aattgctgcc aagcaataag atgtaatggt
     1441 gagtgttcat ttctatttat tcatggcgat acgtgccatc atttctatgc tacgtaattt
     1501 atctttgtat gcattatgag cgttcatttt ttctatatcc aaagctgcat cagaattctc
     1561 gttccaccta cgacacaaat atactgaatt ataaatgcgt ccgatttggt agtttctact
     1621 aatctgaagt cccaaagcat aatcttctcc atagctggta tttggcactt tgatttctct
     1681 taatacagga gtataaaacg ctcgtggagc tcctaatcca ttgatacgaa gtgcattgtt
     1741 tcttccattc tcgggtgtcc attctttgtg gtcaattagt cctggaggaa tgggttgaag
     1801 ggcaaaatct gtcatgctat acgaaccaat taccatggca caattttgtt cgtaaaatgc
     1861 gtttactatc tgttgtagtg ttgttgcgtt ttcgtataag tcatcgctat ctagttgaac
     1921 ggcaaa
//
LOCUS       DAOPUR010000311         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_65152_length_1920_cov_4.357105, whole genome shotgun sequence.
ACCESSION   DAOPUR010000311 DAOPUR010000000
VERSION     DAOPUR010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65152_length_1920_cov_4.357105"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..854)
                     /locus_tag="PKN90_05835"
     CDS             complement(99..854)
                     /locus_tag="PKN90_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNZ85829.1"
                     /translation="MAEKKTEIEISIEQKLRALYDLQTIYSDIDRIKTLRGELPLEVQ
                     DLEDEIIGLQTRITKYDADVEELTNRIVNRKVDIQNATALITKYQEQINNVRNNREFD
                     HLTKEVEYQTLEIEHCEKNIREFTVAIDVKKEEKAKALAQLEDMNANLKEKKEELEEI
                     VSETRQEEEKLRDDAKQLEKLIEDRLLQAFKRIRKNARNGLAVVYIQRDACGGCFNKI
                     PPQRQLDIRLRKKIIVCEHCGRILVDSELANAE"
     gene            complement(902..>1920)
                     /locus_tag="PKN90_05840"
     CDS             complement(902..>1920)
                     /locus_tag="PKN90_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNZ85830.1"
                     /translation="LLIDNENSYITCVLLCLDVTEKVVDEAIQKKCNFILAHHPLIFK
                     GLTKITQQTYTERCVRKAIQHNIAIYAAHTNMDVVMNGVSSMMCKQLGATDLHVLMPT
                     ENGLAKLVCYVPNTYADKIKEALFMVGVGHIGNYDCCSFSSTGIGSFRANNEAHPFVG
                     ECNNLHHENECRIEMILPTRLIKKAEETLKMVHPYEEPAYDVIPLVNRDYSVGLGMVA
                     TLASPMNEMDFMDMLKQKFGCKMLRTSTLTNKMIKKVAVCGGSGRSLIQQAINHKADA
                     YVTADISYHDFFLPENRLLLVDIGHFESEHFIKDIFFDLISKKMPTFAIHFSENEHNP
                     VLYF"
BASE COUNT          599 a          374 c          256 g          691 t
ORIGIN      
        1 atgtatttcc cccatacaaa aataatgaca ttaagtagga taaaaataaa aaaaatagcc
       61 tgacacatat atcaggctat taactataaa aatagaattt attcagcgtt agctagttcg
      121 gaatcaacta aaatacgtcc gcaatgttcg catacaataa tttttttacg taggcgaata
      181 tccaactgtc tttgaggtgg aattttatta aaacaaccgc cacatgcatc acgttgaata
      241 tataccacag ctaaaccatt acgtgcattt ttacgaatac gtttaaatgc ttgcaataaa
      301 cgatcttcta tcaatttttc caactgctta gcatcatctc gaagcttttc ttcttcttgt
      361 ctagtttctg atacaatttc ttctaattct tcttttttct ctttcaaatt agcattcata
      421 tcttctagct gagcaagagc ttttgctttt tcttcttttt ttacatcaat ggcaactgtg
      481 aattcacgta tatttttttc acaatgttca atctcaagtg tttgatattc aacctctttt
      541 gtcaaatggt caaactctcg attatttctt acattattaa tttgttcttg gtactttgtt
      601 atcaaagcag ttgcattctg aatatctact tttctattta caattctgtt tgtcaactct
      661 tctacatcag catcgtattt ggtaatacga gtttgcaaac caatgatttc atcttctaaa
      721 tcttgtactt ctaaaggaag ttcgcctctt aacgttttta ttctgtctat atcagaataa
      781 atggtttgta agtcatacaa agctctgagt ttttgttcta ttgaaatttc aatttcagtc
      841 tttttttcag ccatactaca ggttattttg tgggtattat ctcaaataaa atgcttattt
      901 atcaaaaata gagaacagga ttatgctcat tctctgaaaa atggattgca aatgtaggca
      961 ttttttttga aattaaatca aaaaaaatgt cttttataaa atgttcagac tcaaaatgtc
     1021 caatatcgac caaaagtaaa cgattttcgg gtaaaaaaaa gtcatgatac gaaatatctg
     1081 ctgttacata agcatctgcc ttgtgattta ttgcttgttg tatcaacgaa cgcccactac
     1141 ctccacacac agcaactttt tttatcatct tatttgttaa ggttgacgta cgtagcatct
     1201 tgcatccaaa tttttgtttc aacatatcca taaagtccat ctcattcatt ggagatgcca
     1261 aagtggctac catacctaat cctacagaat agtctctatt tactaacgga ataacatcat
     1321 aagctggctc ttcataagga tgaaccattt tgagagtttc ttcagctttt tttatcaatc
     1381 gagtgggcaa aatcatctct atgcgacact cattctcgtg gtgcaagtta ttacattctc
     1441 ccacaaatgg atgtgcttca ttatttgctc gaaaagaacc aattcctgta gaagagaaac
     1501 tacaacaatc ataattacca atatggccta ctcctaccat aaacaaagct tcctttattt
     1561 tatcagcata tgtgtttggc acataacaga ctaacttagc aagtccattt tctgttggca
     1621 ttaacacatg caaatcagtt gcccccaatt gcttacacat catactactc acgccattca
     1681 tcaccacatc catattggta tgtgcagcat aaatagcaat attatgttgg atggctttcc
     1741 gcacacatct ctctgtatat gtttgttgcg taattttagt taacccttta aaaattaacg
     1801 gatgatgtgc taaaataaag ttgcattttt tttgaatagc ctcatctaca actttctctg
     1861 taacatctaa acaaagtaac acacatgtaa tataactatt ttcgttatca atcagtaaac
//
LOCUS       DAOPUR010000312         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_66908_length_1887_cov_3.297489, whole genome shotgun sequence.
ACCESSION   DAOPUR010000312 DAOPUR010000000
VERSION     DAOPUR010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66908_length_1887_cov_3.297489"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1169)
                     /locus_tag="PKN90_05845"
                     /pseudo
     CDS             complement(1..1169)
                     /locus_tag="PKN90_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203429.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
     gene            1500..>1887
                     /locus_tag="PKN90_05850"
     CDS             1500..>1887
                     /locus_tag="PKN90_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNZ85831.1"
                     /translation="MEDKLVLEVRAKAQRWLDGNYDEETKKQVKAMLDNNDTTDLIES
                     FYKDLEFGTGGLRGIMGVGTNRMNIYTMGAATQGLANYLIKEFGKNSSIKVVLGHDCR
                     NNSRLFAETAANIFSANGIKVYLFEDL"
BASE COUNT          615 a          340 c          319 g          613 t
ORIGIN      
        1 gaacccataa ccaaattaaa acggtgagca ggtgcacttt tatatttctt aatggctaat
       61 tcgcttgcac tttggcttgt tatatcatac ctgtcaatag cttcttgcaa tgttaaacca
      121 tctactcttt taacgctcgt attaattaag ttcccttttg ccaactctgc caaaatacct
      181 aaaataccgc cagcacggtt aacatcttga atgtggtatt tttgtgaatt aggagcaact
      241 ttacacaagc aaggtgtttt acgagataag gcatcaatat catccatatt gaaagaaaca
      301 ttcccttcgt atgcaatggc taaagtatgt aaaatagtgt ttgtagatcc acccatagct
      361 atatccagta ccattgcatt taaaaaagca tctcgtgtag ctatagaacg tggtaaaaca
      421 ctatcgtctc catttgcgta atatttatac gtattatcaa caatcatttt agctgcctca
      481 atgaataatt gttttcgatt agcatgtgtg gcaacaatag ttccattacc tggtaatgct
      541 taaccaatag cttcgtttaa acaattcata gagtttgcag taaacattcc tgaacatgac
      601 ccacaggtag ggcaggctgc ccgttcaatt cgatcaactt cctcgtcaga tacacttgga
      661 tctgccgatt gtatcatggc atcaatcaaa tctaaatgct tgttgtcaag ttctccagct
      721 tccattggac ccccagaaac aaaaattgta ggaatattta atcgcattgc agccattaac
      781 atacctggag taattttgtc gcaattgctt atacatacca tcgcatctgc tttgtgtgca
      841 tttaccatgt attcaacgct atcagctatt aaatcacgac ttggaagcga atataacata
      901 ccatcatgtc ccatagcaat accatcatca atggctatgg tattaaattc agctgcaaaa
      961 cagcctaatt tttcaatttc ttgttttacc aactgaccta tttggtgtaa atgaacatga
     1021 cctggtacaa attgagtaaa cgaattgaca actgcaataa tgggtttccc aatttgattt
     1081 tcttgcatac cgttggcacg ccacaatgct ctggctcctg ccattcttct gccttgtgtg
     1141 cttgtagcac ttcttagttg ttgtttcatt acaatgtttg ttatttacct aaaatatata
     1201 aaaataaaaa aagccctacc acacaggtga gaaggcttat cacatataga aaaatacaca
     1261 cataaccatc tcaccttatg gaactgacca cgaggataat aatgcttaaa atgacggtta
     1321 gacgtgtttt cattgtttaa atcaataatt tagttgcaaa gataggtatt tatttttaaa
     1381 ataaaagtga tttgccttaa aaaatgtaca atcctgaaaa aattacattt tttttatatt
     1441 tcaagggctg attttgaaaa aaaaacgtat ttttgcctga attttttaat aaaacaataa
     1501 tggaagataa attagtttta gaagtaagag cgaaagctca acgatggctt gatggaaatt
     1561 atgacgaaga gaccaaaaaa caagtaaaag ctatgcttga taataatgat actaccgatt
     1621 taatagaatc attttataaa gacttagaat ttggcacagg tggtttacgt ggcattatgg
     1681 gtgttggcac taatcgtatg aatatatata caatgggtgc agcaacacaa ggtttagcta
     1741 attatttgat taaagagttt ggtaaaaatt catctattaa ggttgtgtta gggcatgatt
     1801 gtcggaataa tagtcgcttg tttgccgaaa cagctgctaa tattttttca gcaaatggaa
     1861 taaaagtgta tttgttcgag gatttac
//
LOCUS       DAOPUR010000313         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_67237_length_1881_cov_3.716867, whole genome shotgun sequence.
ACCESSION   DAOPUR010000313 DAOPUR010000000
VERSION     DAOPUR010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67237_length_1881_cov_3.716867"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..161
                     /locus_tag="PKN90_05855"
     CDS             45..161
                     /locus_tag="PKN90_05855"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HNZ85832.1"
                     /translation="MDYTLPIIIPVINLILCYLAIRAIGKDEALIRSLSRIR"
     gene            284..>1881
                     /locus_tag="PKN90_05860"
     CDS             284..>1881
                     /locus_tag="PKN90_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNZ85833.1"
                     /translation="MNTKFSEYTHLDLSAINKDILAYWDKHDIFKKSIDTRTGNPTFV
                     FYEGPPSANGMPGIHHVMARTIKDLFLRYKTMKGYQVQRKAGWDTHGLPVELGVEKSL
                     GITKEDIGKSISVEEYNAACRKDVMKYTKEWESLTHKMGYWVDMNHPYITYDNKFIES
                     IWWLLKELYNKNLLYKGYTIQPYSPAAGSGLSSHELNQPGCYKDVKDTTVVAQFEIDN
                     PIAELTQFGKAYFLAWTTTPWTLPSNTALCVGATITYVAVQTYNPYTFEPQTVILAKD
                     LVSAHFNGKASSLSLSEYTAGDKLIPYSIIAEYKGSELVGMTYKQLIPFFKPDEGAFS
                     VISGDFVTTEDGTGIVHIAPTFGADDARVAKMNNIPAMLLIDKNGNERPLVDLQGKFY
                     TINDIDNDFVAQRTNIALYSPFAGRYVKNAYDTSLNENDATLDIDLCVMLKQQGLAFK
                     IEKHIHNYPHCWRTDKPVLYYPLDSWFIRSTACRETMVELNNTINWKPQATGTGRFGK
                     WLENLQDWNLSRSRYWGTPLPIWRT"
BASE COUNT          646 a          334 c          335 g          566 t
ORIGIN      
        1 tttatattga ttttgccact actatcatta gcaagaacag cattattgat tacacattgc
       61 caattatcat acctgttatc aatcttattc tctgctattt agctattaga gccatcggta
      121 aagatgaagc tttgatacga tccctttcta ggatacgcta aaaaatgaca tatcgttaac
      181 tacaaaaagg cttcttatct ataaaagaag aagctttttt gtaaaaatat ctttttttgt
      241 tctaagttgt ttttttatct ttgcaaattg aaaaaataat gatatgaata caaaattttc
      301 agaatataca catcttgacc tctcggcaat aaataaagat attcttgcct actgggataa
      361 acatgacatt tttaaaaaaa gtattgatac aagaacagga aatcctactt ttgtttttta
      421 cgaaggaccc ccttctgcca acggcatgcc tggcattcac cacgtaatgg cacgtaccat
      481 taaagattta tttttgcgtt acaaaacaat gaaagggtat caagtacaac gcaaagcagg
      541 atgggatact cacggcttac cagtagaatt aggagtggaa aaatctctag gtattaccaa
      601 agaagatatt ggtaaatcta tatcagtaga agagtacaat gccgcatgtc gtaaggatgt
      661 gatgaaatat accaaggaat gggaaagttt aacccacaaa atgggttatt gggtggatat
      721 gaaccatcca tatatcactt atgataataa atttattgaa tctatttggt ggttattaaa
      781 agaattgtac aataaaaatt tattatacaa aggatatacc attcaaccct actcgcctgc
      841 tgctggtagt gggcttagtt cgcacgagct aaatcaaccg ggatgttaca aagatgtaaa
      901 agatactacc gtagttgctc aatttgaaat agataaccct attgcagaat taacccaatt
      961 tggaaaagca tatttcttag catggactac tacaccatgg actttgccat caaatacagc
     1021 cctttgtgtt ggagcaacaa taacatatgt ggctgtgcaa acctataacc cttatacctt
     1081 tgaacctcaa actgttattc tagctaaaga tttggtatct gcccatttta atggaaaagc
     1141 ctcaagccta tcattatccg aatacacagc aggagataaa ttaattccat attccatcat
     1201 agctgaatac aaaggaagtg aactagttgg tatgacttac aaacagctca tacctttctt
     1261 taagcctgat gaaggagcat ttagcgttat tagtggagat tttgtaacaa ccgaagatgg
     1321 tacgggtata gtacatattg cacccacttt tggtgctgat gatgcccgtg tagcaaaaat
     1381 gaacaacatt cctgctatgt tattgataga taaaaatggt aatgaacgcc ccttagttga
     1441 ccttcagggg aagttttata ctattaacga tatagataat gattttgtag ctcaaagaac
     1501 aaatatagcc ctatatagcc cttttgctgg aagatatgta aaaaatgcct acgacacctc
     1561 gctaaatgag aatgatgcaa cgttggatat cgatttgtgc gtaatgctca aacaacaagg
     1621 actagctttt aaaattgaaa aacatataca taactatcca cattgttggc gtacagacaa
     1681 acctgtattg tattatccac tagatagctg gtttatacgc tctacagctt gtcgcgaaac
     1741 aatggttgaa ttaaataaca caataaattg gaaaccacaa gctactggta caggacgttt
     1801 tggaaaatgg ctcgaaaatc ttcaggattg gaatttatct cgtagtaggt attggggaac
     1861 acccctacca atatggcgga c
//
LOCUS       DAOPUR010000314         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_67538_length_1876_cov_2.367930, whole genome shotgun sequence.
ACCESSION   DAOPUR010000314 DAOPUR010000000
VERSION     DAOPUR010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67538_length_1876_cov_2.367930"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PKN90_05865"
     CDS             <1..744
                     /locus_tag="PKN90_05865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ85834.1"
                     /translation="IVPHETHWFDTLFTKWFLKQNTALVTLSEAVRDDLVRLHPAAKH
                     MMKRHPLYNHFGEKISAEQARKQLHIDNNKKTMLFFGLIRDYKGLDLLLDAMNLLDDS
                     YQLVIAGECYGNFDTYQRQIDTNKNKQNIHLFHRYINDSEVPMFFSAADVCVLPYKTA
                     TQSGIAAISYHFEVPLVITNTGGLKEMVETPHTGLVCERIDSDSIAETIQQFFTEDKT
                     SFIEGIQAEKKALSWENFAQSLTEFIHNI"
     gene            complement(745..1230)
                     /locus_tag="PKN90_05870"
     CDS             complement(745..1230)
                     /locus_tag="PKN90_05870"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HNZ85835.1"
                     /translation="MEKIASFTIDHIRLLRGIYLSRTDLVQSETISTFDIRMKEPNRQ
                     PALSIASLHVIEHLAATFLRNHPIWAHKVIYWGPMGCCTGNYLILRGALQPIDIVELM
                     IETFQFIAHFEGDIPGMTPKDCGNCRLQDLPNAKNEAAIFVKEVLSVIQPENMIYPTE
                     K"
BASE COUNT          617 a          373 c          348 g          538 t
ORIGIN      
        1 attgtgcctc acgaaacaca ttggtttgat acacttttta ccaaatggtt tttgaaacaa
       61 aacacagcat tggtaacact tagcgaagcc gtacgagacg atttagttcg tttgcatcct
      121 gctgctaaac acatgatgaa acggcaccca ctatacaatc attttggtga aaaaatatcg
      181 gcagaacaag ctcgaaaaca gctacacatt gataacaaca aaaaaaccat gctatttttt
      241 ggattaatac gcgactacaa aggcttagac ctattgctcg acgccatgaa cctacttgat
      301 gacagttatc agttagtgat tgcaggcgag tgttatggaa attttgacac ataccaacga
      361 caaatagaca cgaacaaaaa caaacaaaac atacaccttt ttcatcgtta tatcaacgac
      421 agcgaagtgc ctatgttttt ttcggctgct gatgtatgcg tattgcccta caaaacagct
      481 acacaaagtg gcattgctgc catttcgtat cattttgagg tacccctagt aattaccaat
      541 acaggaggac taaaagaaat ggtggaaact ccccatactg gactagtgtg cgaacgaatt
      601 gatagtgata gtattgcaga aacaatccaa caatttttca ccgaagataa aacgtccttt
      661 atagaaggta tacaagcaga gaaaaaggct ctttcgtggg aaaattttgc acaatcgctt
      721 accgaattta tacacaatat ctagttactt ttcagtggga taaatcatat tttctggttg
      781 gataactgat aatacttctt ttacaaagat tgcggcttca ttttttgcat tgggtaaatc
      841 ttgcaacctg caattaccac aatctttggg agtcatacct ggaatatccc cctcaaaatg
      901 agcaataaat tgaaaagttt ctatcattaa ttcaacaata tcaatgggtt gcaatgctcc
      961 acgaagtata agataattgc ctgtgcaaca acccatcggt ccccaataaa ttaccttgtg
     1021 tgcccatata ggatgattgc gtaaaaacgt agctgccaaa tgctctatta catgtagcga
     1081 tgcaatagac aaagcaggtt gacgattagg ttctttcatg cgtatatcaa aggtactgat
     1141 ggtttcgctt tgtactaaat cagtacgcga aaggtaaata cctctcaaca gacgaatatg
     1201 atcaatagta aaactggcta ttttttccat atatatatat gataaatatt gttttaagat
     1261 acaaagatag aagtattatt tgttttagcg actaaaatat aattaaaaaa aaaggataga
     1321 gttttttcac tttagaactg tatccaccga ttacaaatcg acttccgcca ttaggttgat
     1381 ttttagccta cctaaaacgc cctgaagagg caatggcgag ctaagagtaa cagaggtgtt
     1441 tagtgtttag tgtttagtgt ttagcgttta gtgtttagtg tttagcgttt agcgtttagc
     1501 gttgttcgta gttcgtgcag aattaacaac tatcaattgt caactttcaa ctctccacag
     1561 tacattcttt cgagagcttt gcttttcgta ggcacagcct acgatgaact aggggtaact
     1621 ccgcccagca atagcaatat gtaatacact accgacagct aagggcagaa gatgccattc
     1681 ggcacgaaaa tcccaaggct acgccgttgg gttgatacta cgtatggctt tcagccaacc
     1741 acaacgccct gaaagggcaa cataacatca acccaacggc atcgctttgg gaaaaatgcg
     1801 aatgcataaa actggacact cgtgcaagag actaactttt aacctcgttt ttcgccacaa
     1861 atataaatca atgtat
//
LOCUS       DAOPUR010000315         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_68219_length_1864_cov_3.407407, whole genome shotgun sequence.
ACCESSION   DAOPUR010000315 DAOPUR010000000
VERSION     DAOPUR010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68219_length_1864_cov_3.407407"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..311
                     /locus_tag="PKN90_05875"
     CDS             45..311
                     /locus_tag="PKN90_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNZ85836.1"
                     /translation="MLRFKWFSLFLYLALGWMIVFVAKPLQENMPLSVFLWIVIGGIF
                     YTAGVYFYVKSAKPYFHSIWHLFVLAGTIAHTIGVYVTLTHSLI"
     gene            complement(303..677)
                     /locus_tag="PKN90_05880"
     CDS             complement(303..677)
                     /locus_tag="PKN90_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ85837.1"
                     /translation="MKFLCALRDLQRAMVLYESSFLNAYGISLNEGMLLCCLSDKQLK
                     ATELAESIELTCSNCSKVIKLAEDKGYIARNIGKEDKRQMLFTLTESGKQKIVEIETN
                     TIALPEVLQDYIKCYENKPNLN"
     gene            complement(687..>1864)
                     /locus_tag="PKN90_05885"
     CDS             complement(687..>1864)
                     /locus_tag="PKN90_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNZ85838.1"
                     /translation="AKDPIAIAGYTAQNVLTGKMPIVHWQTVRDMDSSKIFLLDVRTE
                     DEFQLGSLTGAVNIPLDVLRNHLDELPQDKPIYVFCAIGLRGYLATTILLQRGYKQVY
                     NISGGYKTYETAVSKIVNPSELSQPEKRAYELDLDIQTPKPAKKYLAVDACGLQCPGP
                     ILKMKKSMDDLVEGDRIEMVATDAGFTRDAQAWCNMTGNTLVSQSAKEGKYTVIIEKG
                     NSVIANESTECCAPSSSGKGKTLIVFSDDLDKALAAFVLANGAAATGEKVSIFFTFWG
                     LNTIKKSKKPAVKKDIFGKMFGLMLPASSQKLNLSKMSMFGMGKYMMRFIMKLRGIDT
                     LESLRQQALENGVEFIACQMSMDVMGVKKEELLDEVNIGGVATYMERADKSNINLFI"
BASE COUNT          556 a          371 c          285 g          652 t
ORIGIN      
        1 acatatcata aaacttacaa tttgtcaaat ataaactatt ttttatttta cgttttaaat
       61 ggttttcatt gttcttatac cttgctttgg gctggatgat tgtatttgta gctaaaccgc
      121 tacaagagaa tatgcctttg tcagttttct tgtggattgt aatcggagga attttttata
      181 ctgctggggt atacttttat gtaaaaagtg ccaaaccgta ttttcatagt atttggcacc
      241 tttttgtatt agcaggtaca atagcacata caattggggt atatgttaca cttacccatt
      301 cactaattta agtttggttt gttttcgtaa catttgatat aatcttgtaa gacttctggt
      361 aaagcaattg tatttgtttc aatttcaact attttttgtt ttccactttc agtcaacgtg
      421 aacagcatct gacgcttatc ttctttgcca atgtttcgtg caatataccc tttatcttct
      481 gccagtttaa tcacttttga acaattagaa caagtaagtt caatggattc ggctagttcc
      541 gttgctttta gttgtttatc agataagcaa cataacaaca tgccctcgtt taatgagatg
      601 ccataagcat tcaaaaagct actttcatac aacaccatcg cacgttgcaa atcgcgtaaa
      661 gcacataaaa atttcatagt aatatattaa ataaaaagat taatatttga tttatcggca
      721 cgttccatat aggtagccac accaccaata tttacttcgt ctagtaactc ttcttttttt
      781 acacccatca cgtccattga catttgacaa gcaataaact ctacaccatt ctccaaggct
      841 tgttgacgta aagattccaa ggtatcaata ccacgtaatt tcattataaa ccgcatcata
      901 tatttcccca tgccaaacat actcattttt gataaattga gtttttggga gctagcaggt
      961 aacataagtc caaacatttt accaaagata tcttttttta cagcaggttt ctttgatttt
     1021 tttatggtat ttaatcccca aaaagtaaag aaaatggata ctttctctcc cgttgctgca
     1081 gccccattgg ctaatacaaa agcagctaag gctttatcta aatcatcact aaatacaata
     1141 agtgttttcc ctttacctga tgaagatggg gcacaacatt ctgttgattc gttggcaata
     1201 acactatttc ctttttcaat aattacggta tatttcccct cttttgctga ttgtgataca
     1261 agggtattgc cagtcatatt acaccaagct tgtgcatctc gcgtaaaacc agcatcggta
     1321 gcaaccattt caatacgatc tccttctacc aaatcatcca ttgatttttt catctttaag
     1381 attggaccag gacattgtaa tccacaagca tctacagcta ggtatttttt agcaggtttt
     1441 ggtgtttgta tatctaaatc aagctcatac gctcgctttt cgggttgaga taattcgctc
     1501 ggattcacaa tcttactgac agctgtttcg tacgttttat agcctccaga aatgttataa
     1561 acttgcttat atccacgttg aagcaatata gtagtcgcta aataccccct taatccaatg
     1621 gcacaaaaaa cataaatggg tttatcttga ggcaattcgt caaggtgatt gcgtaacaca
     1681 tccaagggta tattcacagc tccagttaaa ctaccaagtt gaaattcatc ttcggtacgc
     1741 acatctagca aaaaaatctt actgctatcc atatcacgta cagtttgcca atgtactata
     1801 ggcatttttc ctgttaatac attttgagca gtatagcctg caatagcaat aggatcttta
     1861 gccg
//
LOCUS       DAOPUR010000316         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_68245_length_1864_cov_2.301824, whole genome shotgun sequence.
ACCESSION   DAOPUR010000316 DAOPUR010000000
VERSION     DAOPUR010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68245_length_1864_cov_2.301824"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKN90_05890"
     CDS             complement(<1..200)
                     /locus_tag="PKN90_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85839.1"
                     /translation="MKKSFFLFFLFLFSLSFFAQQLVTTTPAFIAQNYAGEIKVVFDA
                     TQGNDAMLGSATCYAHAGVILTS"
     gene            complement(449..>1864)
                     /gene="secF"
                     /locus_tag="PKN90_05895"
     CDS             complement(449..>1864)
                     /gene="secF"
                     /locus_tag="PKN90_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNZ85840.1"
                     /translation="VGILASFKAVLTLPGIAGIVLTLGMAVDANVLIYERIKEELAAG
                     KTHKSAIADGYKNALSAILDSNITTIITAVILFYFGTGPIKGFATTLIIGIVTSFFTA
                     VFVTRWAYETLLNKNFTEKLSFTTKVSANFLSNVNLDWVKLRKVGYILSITFMVVAIF
                     SLAFHGLNQGIDFTGGRNYVIKFEESVSTQDLQRKLALGFEDASVSVITIGAVSENQV
                     RISTNYKIQDNSDTVDDEIENVLYEQLKPFIKNQTVTRDMFVKGYVINEDGDAQLSSA
                     GSVRPLGVQSSQKVGPAIADDIKNAAYLAIFFSLIGIGLYILLRFKDISYSASIVVSL
                     IHTSVFVLGVYSLFYSVMPFSMEIGQSFIAAILTVIGYSVNDTVVVFDRLRETIGLYP
                     KRDRKEMFNYSVNATLARTFNTTFSTLLVLIIIFLLGGDTIRGFVFALLLGITIGTYS
                     TLFVSVPLAYDIQMKTLNKKK"
BASE COUNT          689 a          362 c          268 g          545 t
ORIGIN      
        1 gaggttagta taacccctgc atgcgcataa catgtagctg acccaagcat ggcatcatta
       61 ccttgagtag catcaaaaac aacttttatt tcgccagcat aattttgagc aataaatgcc
      121 ggagtagtcg taactagttg ctgagcaaaa aaagaaagac taaaaaggaa caaaaaaaat
      181 agaaaaaaac tttttttcat acgatgatgt tttcaagtat aaagacacac acctcactcc
      241 acaaactatg tatcatttat aaaaaatgta tacaaaaaca agctgaatgc cgtctactta
      301 ttgtgaacaa atataattaa tatacaataa taattgcaaa aatctttcgc aagatacgaa
      361 atatttttgg ataaagcaat tgattagatg cattttaaca aaaaaggact cacttatatg
      421 aatccttttt tgttaaaata ctataccctt acttcttctt atttaacgtc ttcatctgta
      481 tatcgtacgc taatggtaca gaaacaaaca atgtagaata cgttccaatc gttattccta
      541 ataacaaagc aaacacaaat cccctaattg tatctccacc aagtaagaaa ataattatca
      601 aaaccaataa ggtagaaaaa gtggtattaa atgttctggc taaggttgca tttaccgaat
      661 agttaaacat ttcttttcta tcccgtttcg gatacaaccc aatcgtttct ctcaatcggt
      721 cgaatactac taccgtatca tttaccgaat acccaattac ggtaagtata gcagcaataa
      781 atgattgtcc aatttccata gaaaaaggca tcacactata gaacaatgag tacactccca
      841 atacaaatac agaggtatga attaaagaaa ccacaatact tgctgaataa gatatatctt
      901 taaatcgcaa aagaatatac aatccaatac caatcaatga aaagaagata gccaagtagg
      961 ctgcattttt gatgtcgtca gcaatagctg gaccaacttt ttgcgaactt tgcacgccta
     1021 atggacgcac tgaaccagca ctagacaatt gggcatcgcc atcttcattt ataacatacc
     1081 ccttcacaaa catatcacga gtaactgttt gattcttgat aaatggcttt aattgttcat
     1141 acaagacatt ttcaatttca tcgtccacag tatcactgtt atcttgaatt ttatagtttg
     1201 tagatatccg tacttgattt tcagaaacag caccaatagt aattacagat acagaagcat
     1261 cctcaaaacc aagagccagc ttacgttgta ggtcttgtgt acttacgctt tcttcaaatt
     1321 ttatcacgta attacgacca ccagtaaaat caattccttg attcaaacca tggaaagcca
     1381 acgagaatat agccactacc ataaaggtaa tagaaagaat atagcctact ttacgtaatt
     1441 tcacccaatc aagattgacg ttacttaaaa aattggctga aactttggtt gtaaaagata
     1501 atttttctgt aaaattttta ttcaataacg tttcgtaagc ccaacgagta acaaatacgg
     1561 ctgtaaaaaa tgatgtaacg atacctataa tcagtgtagt agcaaatcct tttattggac
     1621 cagtaccaaa ataaaataat attacggcag taataatggt tgtaatattt gaatccaaga
     1681 tagcagataa agcatttttg taaccatcgg caattgctga tttatgtgtt tttccagctg
     1741 caagttcttc tttaatacgc tcataaatca acacattggc atctaccgcc atacccaacg
     1801 taagcacaat acctgctata cctggcaagg ttaatacggc tttaaacgag gctaaaatac
     1861 caac
//
LOCUS       DAOPUR010000317         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_69109_length_1849_cov_2.885730, whole genome shotgun sequence.
ACCESSION   DAOPUR010000317 DAOPUR010000000
VERSION     DAOPUR010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69109_length_1849_cov_2.885730"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1539
                     /locus_tag="PKN90_05900"
     CDS             205..1539
                     /locus_tag="PKN90_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNZ85841.1"
                     /translation="MNAKNVIENLKKRFPNEPEYLQAVEEVLESIEEAYNQHPEFEKA
                     NLIERLCIPDRIFTFKVSWVDDKGNVQVNMAYRVQHNMAIGPYKGGIRFHSSVNPSIL
                     KFLAFEQTFKNALTTLPMGGGKGGSDFHPRGKSSGEVMRFCQAYLLELHRHIGPHTDV
                     PAGDIGVGGREVAYMFGMYKKLTHEFTGTFTGKGIEFGGSLIRPEATGYGNIYFLLNM
                     LATRNIDIKGKKCLVSGSGNVAQYTCEKLIELGAIPVTLSDSNGYIYDPEGITREKLE
                     YVMDLKTVQRGRIKEYADKYNCKYVEGGRVWAEKGDIALPSATQNELNGDDAKTLMAN
                     GCIAVSEGANMPSTPEAIEIFLKNKILYGPGKAANAGGVATSGLEMSQNAIGLSWSRE
                     EVDARLKGIMNNIHENCVKYGTEKDGFVNYVKGANIAGFMKVAKAMMAQGIV"
     gene            1641..>1849
                     /locus_tag="PKN90_05905"
     CDS             1641..>1849
                     /locus_tag="PKN90_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNZ85842.1"
                     /translation="MNKIISKEHFSANVVKLEVEAPMIAKSRRAGHFVLVKVGEKGER
                     IPLTITSSDLEKGTITLVIQKMGVSS"
BASE COUNT          642 a          277 c          375 g          555 t
ORIGIN      
        1 catgttgaaa ggagcatgaa aaatatgcaa tctattacaa ttaacgccat aattctttta
       61 ctctataatc aataacaggt tttcagtttt tatttaaaat taagaactag aatatacaat
      121 aaaaacagta acatttattt tgtcaaaagg ataaaatgtg tatttttgcg tcagagtaga
      181 ttttttttaa aacacattta atttatgaac gcaaaaaacg ttattgaaaa tctcaaaaag
      241 agatttccaa atgagcctga gtacttgcag gcagttgaag aagtattaga gtctatcgaa
      301 gaagcgtaca atcaacaccc tgagtttgaa aaagcaaact taattgaaag attgtgtatt
      361 cctgatagaa tttttacatt taaagtatcg tgggttgacg ataaaggcaa tgttcaagta
      421 aacatggctt atcgcgtaca acacaacatg gctattggac cttacaaagg aggaattcgt
      481 ttccactctt ctgtaaatcc atcgattttg aagtttttag cgtttgagca aacgttcaaa
      541 aatgcactta caacactacc aatgggtggt ggtaaaggtg gatctgattt ccacccaaga
      601 ggaaaatcat ctggcgaggt tatgcgtttt tgtcaagctt atttattgga acttcatcgt
      661 catattggtc ctcacactga cgtacctgct ggtgacatag gtgtaggtgg acgcgaggtt
      721 gcttatatgt ttggtatgta caaaaaactt actcacgaat ttactggcac ttttacaggt
      781 aaaggaattg agtttggtgg ttcattaatt cgtccagaag caactggtta cggaaatatc
      841 tacttcttat taaatatgtt agctacacgc aacattgata ttaaaggtaa aaaatgttta
      901 gtatctggtt cgggtaacgt agctcaatat acttgtgaaa aattaattga attgggagca
      961 attcctgtaa cactatctga ttcaaacggt tatatctacg atccagaagg tattacacgt
     1021 gaaaaattag aatacgtaat ggatttaaaa accgtacaac gtggacgtat taaagaatat
     1081 gccgacaaat acaattgtaa atacgtagaa ggtggtcgtg tatgggctga aaaaggtgat
     1141 atcgctttac catcagctac tcaaaacgaa ttgaatggcg acgatgcgaa aactcttatg
     1201 gcaaacggtt gtattgctgt atcagaagga gcaaatatgc cttcaactcc agaagcgatt
     1261 gagatattct tgaaaaacaa aattctttat ggacctggaa aagcagccaa tgcaggagga
     1321 gtggctactt ctggtttaga aatgtctcaa aatgctatcg gattgagctg gtcgagagaa
     1381 gaagttgatg cccgtttgaa aggtattatg aacaacattc acgaaaactg tgtaaaatac
     1441 ggaacagaaa aagacggttt tgttaattat gtgaaaggag caaatatagc aggctttatg
     1501 aaagtagcta aagctatgat ggctcaaggt attgtataga gtacacatac ttgtttatat
     1561 atttcattgc acgttgtcga cattcaagat attgataatg tgcaatgttt tgctgtttaa
     1621 aatcttttaa tttttataga atgaataaaa taattagtaa agaacacttc tctgccaatg
     1681 tagtaaagtt agaagttgaa gcaccgatga tagctaaatc acgtcgcgct ggacattttg
     1741 tactagtcaa agtgggagaa aaaggggaac gcattccgtt aacaatcaca tcgtctgatt
     1801 tagaaaaagg gacaataaca ttggtaatac aaaagatggg agtttcatc
//
LOCUS       DAOPUR010000318         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_69157_length_1848_cov_3.844953, whole genome shotgun sequence.
ACCESSION   DAOPUR010000318 DAOPUR010000000
VERSION     DAOPUR010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69157_length_1848_cov_3.844953"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PKN90_05910"
     CDS             complement(<1..1081)
                     /locus_tag="PKN90_05910"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNZ85843.1"
                     /translation="MSYKNLDQQNLSFLKELLSTNSPSGYEVNAAKVWQNYVNQFAEV
                     SGDVLNNSIGVLNPQAEFKIMIAGHYDEIGLQVMYISKEGYLYVRRLGGVDLLSLPGS
                     EVVVITKKGTISGVLGKIPIHVQSPTERTKAIELEDLWIDIGVDSEEEAANLVEVGDP
                     VAIKSNFHYLGQHKIVSKGMDDKIGAFIVAEVLRNLSTQQLKVGVYGVATSQEELGAR
                     GAITSTFKVNPHVGFCLDVGIATDVPGIEERKYGKMRLGGGASITHSADNNVVLVRLL
                     QQTAQQNNIQYQDVASKSATGGTDTAHMQLARQGVATALISVPNRYMHTQVEMCDLRD
                     VDEIISLLVETIKVIDKDAIVDFVPS"
     gene            complement(1127..1585)
                     /locus_tag="PKN90_05915"
     CDS             complement(1127..1585)
                     /locus_tag="PKN90_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ85844.1"
                     /translation="MPEITNEITLMLFKNEISAEFAQQLCEDADKVLEFVSQVKWKDG
                     FVCRNCNHTNYCEGKTPFSRRCTRCKKEESATAHTLFHNIKFPINKAFFIAYHVCMLG
                     DEFSSYTYSFQLGLNQMTCWKFRKRIQHCIAKVDKQDSNTIQGILLQTYE"
BASE COUNT          546 a          377 c          296 g          629 t
ORIGIN      
        1 gagatggaac aaaatctaca atagcatctt tgtctattac tttgattgtt tcaactaata
       61 aggaaataat ttcgtctaca tctctcaaat cgcacatttc tacttgcgta tgcatatatc
      121 tattgggcac gctgattaat gctgtggcta ccccttgtct agcaagttgc atatgagcag
      181 tatctgttcc tcctgttgct gatttagaag ccacatcttg gtactggatg ttgttttgct
      241 gtgctgtttg ttgtaacaac cttactaaaa ccacattatt atctgcacta tgggtaatag
      301 aagctcctcc gccaagtctc attttgccat attttcgttc ttctatacct ggcacatcgg
      361 tagcaatacc cacgtctaaa caaaaaccta cgtgtggatt taccttaaaa gtactcgtaa
      421 tagcacctct ggctcccaat tcttcttgtg aggtagctac accatatacg cctactttaa
      481 gttgttgtgt tgataaatta cgaagaacct cagctacaat aaatgcacca attttgtcat
      541 ccatcccttt agatacaatt ttgtgttgac ctaaataatg aaaattggat ttaatagcta
      601 ctggatcgcc tacttcaacc aaattagcag cttcctcttc cgaatctacg cctatgtcta
      661 tccataaatc ttctaactca atggctttgg ttcgctcagt tggcgattgc acatgaatgg
      721 ggattttacc caacacgcca cttatagtac cttttttggt aatcactacc acttcactac
      781 ctggcaaact caacaagtcg acacctccca gcctacgcac atataagtaa ccttctttag
      841 aaatatacat cacctgcaaa cctatttcgt cataatgacc tgcaatcata attttaaatt
      901 ccgcttgggg attaagcacc cctatagaat tatttaacac atcaccactt acttctgcaa
      961 attgatttac atagttttgc catacttttg cagcgtttac ttcgtaacct gaaggagaat
     1021 ttgttgaaag taactctttt aaaaaagata gattttgctg gtctaaattt ttgtaactca
     1081 tcgtttttta tttaattagt atactatttg attatataac acgccattat tcatacgttt
     1141 gtaacaaaat cccttgtatg gtgtttgagt cctgtttatc caccttagca atacagtgct
     1201 gaatccgttt tctgaatttc caacaagtca tctgattgag tcctaactga aaagaatacg
     1261 tataggacga aaactcatct cctaacatac acacatgata ggctataaaa aaagccttgt
     1321 taatcggaaa ttttatgtta tgaaacaagg tatgagccgt tgccgattct tcttttttac
     1381 aacgcgtaca acgccttgaa aagggggttt ttccttcgca ataattagta tggttgcaat
     1441 tgcggcacac aaaaccatct ttccatttta cttgcgacac aaactccagc actttatcag
     1501 catcttcgca taattgctgt gcaaactcag cagatatttc gttcttaaaa agcatcaatg
     1561 ttatttcgtt tgtaatttct ggcaaatata tacatttttt tagtgataat aaaataattt
     1621 atagtgatac tattgtaata taaaataatt tttgtatttt tgcacattat ttacaataaa
     1681 caagtatctt tatgataaca gatgcaatca acaaacgata ttcttcatta agcgaaacga
     1741 cgtgctgttt gtcttgcgga ggtgctataa actatgccaa tccattggtt ggtgaagtgt
     1801 gtgtagattt gggcagtggc agaggaaatg atgttgtacg tttggcag
//
LOCUS       DAOPUR010000319         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_69242_length_1847_cov_2.640067, whole genome shotgun sequence.
ACCESSION   DAOPUR010000319 DAOPUR010000000
VERSION     DAOPUR010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69242_length_1847_cov_2.640067"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKN90_05920"
     CDS             <1..978
                     /locus_tag="PKN90_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ85845.1"
                     /translation="HFLDAVSTHIVDIDYGKIQLVPGNYSFWYESSQLALRQQQAQNK
                     KAEEKKKELEEFIRRFSANVAKSKQATSRRKMLEKLNVEEIQPSTRKYPGIIFTPDRE
                     PGNMVLEVKGLSHSMEGQVLFKDVNFNVEKGDKIVFLSKDPRAMTAFFEIINGNIKPD
                     SGTFNWGVTITSAYLPLNNVDFFNTDLNLVDWLGQFSDDTTESFIRGYLGRMLFSGEE
                     IYKKAGVLSGGEKMRCMISRMMLKNANVMVLDSPTNHLDLESIQAFNNSLKSFKGNIL
                     MSSHDHEFIQTVCNRVIELTPNGIIDKVIEYDDYLDDMHVKTQREKLYK"
     gene            1139..>1847
                     /locus_tag="PKN90_05925"
     CDS             1139..>1847
                     /locus_tag="PKN90_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85846.1"
                     /translation="MNKATYSILCIVLLLTSCNLRDKEVIDPDYFFKNDSNLSLHTVV
                     ITDKATRLTFSILTESPTQIKIDSTAYLVANNKKYPLLENTLNVIAIDSGKYSFSLEF
                     EPLPKSTTSFDFIECEQESCIQMIGILLKGTYKDAEYTKKIPIQLVSANYSEAKMPKM
                     EMKCGSTQVIMHLVSPLPVDSSLFKGIISVFDITTGSYTDYQSNSDSLQTFSWEYEQC
                     SPGIVVFKIENNPIQLFV"
BASE COUNT          657 a          297 c          323 g          570 t
ORIGIN      
        1 cactttttag atgctgtaag tacccatatt gtggatattg attatggcaa aatacaatta
       61 gtgccaggta actattcttt ctggtacgaa tctagtcagc tggctttgcg tcagcaacaa
      121 gcacaaaaca agaaagcgga agaaaagaaa aaggaattgg aagagtttat tcgtcgtttt
      181 agtgccaacg tggctaaatc aaaacaagcg actagccgta gaaaaatgct tgaaaaacta
      241 aatgtagagg aaattcaacc atctacacgt aaatatccag gtatcatttt tacacccgat
      301 cgcgaacctg gcaatatggt actggaagtg aaaggacttt cgcatagcat ggaaggtcaa
      361 gtattgttta aggatgtaaa ctttaacgta gaaaaagggg ataaaattgt atttttatcc
      421 aaagacccta gggctatgac tgcgtttttc gagattatca atggcaatat aaaaccagac
      481 agtggaacat ttaactgggg agtaaccatt acctcagcct atttaccgct taataatgtt
      541 gattttttta atactgattt gaatttagta gattggttgg gacaattttc tgacgatact
      601 accgaatcat ttatacgtgg ctatttgggc agaatgttgt tttctggcga agaaatttat
      661 aaaaaagcag gcgtattatc aggaggagaa aaaatgcgtt gtatgatttc gcgtatgatg
      721 ctcaaaaatg ccaatgttat ggtgctcgac tctcctacca accacttgga tttggaatcg
      781 attcaagcat ttaacaactc gctaaaatca tttaagggaa atatcctgat gtcgtctcac
      841 gaccatgagt ttattcaaac agtatgtaac cgtgtaattg aactaacgcc caatggcatt
      901 attgataaag ttatagaata cgatgattac ttagacgata tgcacgtaaa aactcaacga
      961 gaaaaattat acaaataaat ttgttataac aaacaataaa acctcctata ctactttgtt
     1021 taggaggttt ttttattatt ttaataatcg atatactatt attactcttt tcacgtttat
     1081 agaagtaagt tgaaagacac aaacaataat tttttttgaa atttaatact aaaactctat
     1141 gaacaaagca acatattcca tattgtgtat tgtcctatta ttaactagtt gtaacctacg
     1201 agataaagaa gtaatagacc ctgattattt ttttaaaaac gatagcaatc tttcattaca
     1261 cacagtagta attacagata aagcaacacg tctaactttt tcaatactca ctgagtcgcc
     1321 aacacaaata aaaattgatt caacggcgta tttagttgca aataacaaga aataccctct
     1381 tttagagaat acgctaaatg ttatagcaat tgattctggc aagtattctt tttcgctaga
     1441 atttgaaccg ttaccaaaat caacaacatc ctttgacttt attgaatgtg agcaggaaag
     1501 ttgtatacaa atgattggaa ttttgttaaa agggacctac aaagatgctg agtatacgaa
     1561 aaaaataccc atacagttag tttctgccaa ttattcagaa gctaaaatgc caaaaatgga
     1621 aatgaaatgt ggtagtacgc aggttataat gcacttagta agtccactac ctgttgattc
     1681 ttcgctgttt aaaggaataa tatctgtttt tgacataaca acagggtcgt atactgacta
     1741 ccaatcaaat agtgattctt tacaaacatt cagttgggaa tatgagcaat gtagtcctgg
     1801 aatagtagtt tttaaaatag aaaacaatcc aattcaacta ttcgttg
//
LOCUS       DAOPUR010000320         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_69922_length_1835_cov_3.884831, whole genome shotgun sequence.
ACCESSION   DAOPUR010000320 DAOPUR010000000
VERSION     DAOPUR010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69922_length_1835_cov_3.884831"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PKN90_05930"
     CDS             <1..801
                     /locus_tag="PKN90_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ85847.1"
                     /translation="MAMGIPDLWIKYIKEYTDENWKPGHLFWELTNRRYDEKTISYAE
                     SHDQALVGDKTIIFRLIDADMYWHMEKGHSTYMVDRGVALHKMIRLLTASTINGGYLN
                     FMGNEFGHPEWIDFPRQGNGWSYKYARRQWSLVDNPNYLYHSLAMFDRGMIDILTKTD
                     KFQDSGVYKYWDHSGDQVLAFARGGELLFVFNFHPYNSFTDYGMLVDKGEYEVVLNTD
                     AKEYGGFGLCDDSIRHFTIPTNVKGEEKEWIKLYLPSRTAMVLRKVSK"
     gene            803..1525
                     /locus_tag="PKN90_05935"
     CDS             803..1525
                     /locus_tag="PKN90_05935"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNZ85848.1"
                     /translation="MFYQDSIHKKSVNQKQLALLVDPDKYSQESLALFVELAKETLPD
                     CIMVGGSLVAGSVEQVVQYIKSHTNIPVVLFPGNSQLCNEADALLLLSLLSGRNADFL
                     IGQHVVAAKAIRDSGIESISTGYILIDGGCSTSVEYMSNTKPIPRDKVEIIVATAIAG
                     ELIGNRLVYLEAGSGAINPVSKEAIQAVRQAISLPIIVGGGIRTVEQAKDAYLAGADM
                     IVVGNALEKDRFFMRDLAQIRG"
     gene            complement(1589..>1835)
                     /locus_tag="PKN90_05940"
     CDS             complement(1589..>1835)
                     /locus_tag="PKN90_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ85849.1"
                     /translation="IMILDEATSALDTESEKLVQEAIEKLMKNRTSVVIAHRLSTIKN
                     ADEICVLLDGKIVERGTHEALLQENGVYKKLQETQIV"
BASE COUNT          555 a          284 c          393 g          603 t
ORIGIN      
        1 atggcaatgg gtattcctga tttatggata aaatacataa aagaatatac tgacgaaaat
       61 tggaaaccag gtcatttatt ttgggaatta accaacagaa gatacgacga aaaaacaatc
      121 agttatgctg aaagtcatga tcaagcttta gtgggtgata aaacaattat tttccgtttg
      181 attgatgccg atatgtattg gcacatggaa aaaggacatt ctacctatat ggtagacaga
      241 ggtgtggctt tacacaaaat gattcgatta cttacagcat caactatcaa tggtggatat
      301 ttaaacttta tgggcaatga gtttggtcat ccagaatgga ttgattttcc acgtcaaggt
      361 aatggttggt cgtataaata tgctcgtaga caatggagct tggtagataa ccctaattat
      421 ttatatcact ctttggcaat gtttgataga ggtatgattg atatattaac caaaacagat
      481 aaatttcaag attcaggtgt ttataaatat tgggatcatt ctggcgatca ggtacttgcg
      541 tttgcacgtg gtggtgagtt attgtttgta tttaattttc atccctataa ttcgtttaca
      601 gactatggca tgttggtaga taaaggggaa tacgaggttg tattgaatac agatgcaaaa
      661 gaatacggtg ggtttggatt atgtgatgat agtattcgac attttaccat accaactaat
      721 gtgaaagggg aagaaaaaga gtggattaaa ctctatctcc catctcgtac agccatggtt
      781 ttacgcaaag tctcaaaata aagtgtttta tcaagatagt atacataaaa aatcagtcaa
      841 tcaaaagcag ttagcactac ttgtcgatcc tgataaatat tctcaagaat cacttgcatt
      901 atttgttgag ttagctaaag aaacattacc tgattgtatt atggtgggtg gcagtttggt
      961 ggctggttct gtagagcaag tagtgcagta tatcaaatca cataccaata ttcctgtggt
     1021 gctatttcct ggaaatagtc aattgtgcaa cgaagccgat gctttattgt tgttgtcgtt
     1081 gttatctggt cgcaatgctg attttttaat tggtcagcat gtagtagcag caaaagccat
     1141 acgcgattcg ggtatcgaat ctatttctac ggggtatatt cttattgatg gaggatgttc
     1201 tactagtgtg gaatatatga gtaacactaa accaattcca cgtgataaag tagaaattat
     1261 tgtagccaca gccatagctg gcgaactaat tggcaatcgt ctggtgtatt tagaagctgg
     1321 aagtggagcc attaatcctg tatcaaaaga agccattcag gcagtaaggc aagcaatttc
     1381 actgcccatt attgtaggtg gaggtatccg cacagtagag caggcaaaag atgcgtatct
     1441 tgctggagct gatatgattg tagtaggcaa tgccctagaa aaagataggt tttttatgcg
     1501 tgatttggca caaattagag gctgagctaa cgatttattt gcgttctcat tacgatattg
     1561 actgttaggt tttgaccgat tttgtgtatt acactatctg cgtttcttgc agttttttgt
     1621 acaccccatt ttcttgcaac aaagcttcgt gtgtgcctct ttctactatt ttaccatcaa
     1681 gaagaacaca aatttcgtca gcatttttga tagtagatag tcggtgagct ataacaacac
     1741 tggttctatt tttcattagt ttttcgatgg cttcttgcac taatttttct gattcagtgt
     1801 ctaaagctga ggtagcttcg tctaaaatca taatt
//
LOCUS       DAOPUR010000321         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_70285_length_1829_cov_3.910372, whole genome shotgun sequence.
ACCESSION   DAOPUR010000321 DAOPUR010000000
VERSION     DAOPUR010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70285_length_1829_cov_3.910372"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..>1829
                     /locus_tag="PKN90_05945"
     CDS             228..>1829
                     /locus_tag="PKN90_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNZ85850.1"
                     /translation="MYAEQIIHRNENRIKVVLPYDSLLVSKLRTIQGASWSQTLKAWH
                     VPHNKVTVTQLRTLFPALDFRQAKPIKNEKSISDNTKELAEHTIEQTKNATNQLDNIQ
                     DKQLDDNQIDIRNKANIHITITDKRLYIKLPKNDTDTQFLSSFRFVQWDKNNLQWILP
                     NYGKNLELIQSYFDKRSVDIHHEKERKISSNSTPHAETGKFKVYALHNRILRIYTTYH
                     RSVINELKQLKLCKWNSTENCWTLPYNEQVLPTLQEIASTYNLQHDYIVVSKTEGSPR
                     LPKHENYLRCPIEYIEKLKELRYSSNTINVYTDLFEEFINYYPTKQVSLITEEEIISF
                     LRYLVNDRKISTSYQNQSINAIKFYYERVLGGKRKIYLIERPRKEKYLPEVLSEEEIA
                     AILKAITNTKHKALIMTIYSGGLRISELINLKIKDIDSDRMQIRIAQSKGKKDRYTLL
                     SKKTLITLRKYFKEYKPKEWLFEGEGGGQYADRSIQNVLKVAVKKVGIKKHITVHTLR
                     HSFATHLLENGTDLRYIQSLLGHSSS"
BASE COUNT          746 a          351 c          284 g          448 t
ORIGIN      
        1 atactatttt ttttgaattc aacaaaggca ggatacttcg ttatcttgcc tttttgattt
       61 gatggagatg gctattgcct gtgaaacaga aaaaaccaca atacgaacaa caaaaaaaaa
      121 gaccgataca ctttgctacc taaaaaaact ttagtagatt tacaacacta attaatgagc
      181 tacacacaat agacttaaat acccactacc gttctacaat aacaaccatg tacgccgaac
      241 aaataataca ccgaaatgaa aaccgcataa aagttgtttt gccctacgat tcattgttgg
      301 taagcaaact acgaactata caaggtgcca gttggagcca aaccctaaaa gcttggcacg
      361 tgccgcataa caaagttacg gtaacacaac taagaacctt attcccagca ctagatttca
      421 gacaagcaaa gccaataaaa aacgagaaaa gcatcagtga taacacaaag gaattagctg
      481 aacatacgat agaacaaacc aagaatgcaa caaatcagtt agataatata caagataaac
      541 aacttgatga taaccaaata gatatccgca acaaagcaaa tattcacatc accattactg
      601 acaaacgcct atatatcaaa ttgcccaaaa acgatacaga tacacaattt ttaagctctt
      661 ttaggtttgt gcaatgggat aagaacaact tacaatggat actaccaaat tatggtaaaa
      721 atctggaatt gatacaatcc tattttgaca aaagaagtgt tgatatacac cacgaaaaag
      781 aaagaaaaat atcaagtaac tctaccccac atgccgaaac aggtaaattt aaagtatatg
      841 ccctacacaa tcgcatacta agaatatata ctacctacca ccgctcagtg attaacgaac
      901 ttaagcaact taagctatgt aaatggaaca gcacagaaaa ctgctggaca ctgccttaca
      961 acgaacaggt attgcctaca ctacaagaaa tagccagtac atacaactta cagcatgatt
     1021 atatagtggt atcaaaaaca gaaggttctc cacgtttgcc caaacacgaa aattacctgc
     1081 gttgtcctat cgaatacatc gaaaaactaa aagaactacg gtatagtagc aacaccatca
     1141 atgtatatac cgatttattt gaggaattta ttaactatta tcctacaaaa caagtttcgc
     1201 tgataactga agaagaaatt atcagttttt tacgttattt ggtaaacgac cgaaaaattt
     1261 ccacctctta tcaaaaccaa tctattaacg ccataaaatt ttactatgag cgagtattag
     1321 gcggaaaacg taaaatatac ctcatcgaac gcccccgaaa agaaaaatac ctacccgaag
     1381 tattgagcga agaagaaatt gccgccatac ttaaagccat taccaacacg aaacataaag
     1441 cattgataat gactatctat tcaggtggat tgcgtataag cgaactcatt aatcttaaaa
     1501 taaaagacat tgatagcgac cgtatgcaaa taagaattgc acaaagcaaa ggaaaaaaag
     1561 accgttatac tttgctaagt aaaaaaactt taataacttt acgcaagtat tttaaggaat
     1621 ataaaccgaa agagtggttg tttgaaggag aaggtggagg acaatatgca gatagaagca
     1681 tacaaaatgt actaaaagtg gcagtaaaaa aagttggcat aaagaaacat ataacagtcc
     1741 atactttacg ccattcattt gcaacacatc ttttagaaaa tgggacagac ttaagatata
     1801 tacaaagttt attgggtcat tcgagtagt
//
LOCUS       DAOPUR010000322         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_70708_length_1822_cov_3.653084, whole genome shotgun sequence.
ACCESSION   DAOPUR010000322 DAOPUR010000000
VERSION     DAOPUR010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70708_length_1822_cov_3.653084"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKN90_05950"
     CDS             complement(<1..259)
                     /locus_tag="PKN90_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85851.1"
                     /translation="MMNPDSQFDDIRCYNDAEIPLQFSKLVEDKQLLAILPQILPNIP
                     VSVLIEQLKKVQSVDQFQKGFILPFLSDLETKTSKGVELIQL"
     gene            333..797
                     /locus_tag="PKN90_05955"
     CDS             333..797
                     /locus_tag="PKN90_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNZ85852.1"
                     /translation="MAKEIERKFLVDKTKWQAVSKGMLLSQGYLSATENTTVRVRTTA
                     DKGFLTIKGKTQRFSRDEYEYIIPLQDAQEMLTMCSDKLVTKYRYTEKHNDMLWEIDV
                     FTDKNEGLIIAEIELTSEEQKFVLPDWVEKEVTGDIRYYNSFLAKTPFSTWE"
     gene            complement(809..1255)
                     /gene="rpiB"
                     /locus_tag="PKN90_05960"
     CDS             complement(809..1255)
                     /gene="rpiB"
                     /locus_tag="PKN90_05960"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNZ85853.1"
                     /translation="MKALDTLYIGIASDHAGYEMKERLKEYFAHTFFALKDFGTHSEE
                     SVDYPDYIHPLAQAVAKNEIDFGIAICGSGNGVSIVANKHKNVRAALCWTDEIAILAR
                     KHNDANIISLPARFISQALAEDMVERFLTTDFEGGRHCNRVNKINE"
     gene            1438..>1822
                     /locus_tag="PKN90_05965"
     CDS             1438..>1822
                     /locus_tag="PKN90_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85854.1"
                     /translation="MSDSISLYFNDVLVNPTLPSYMSENEYIASNLHTQDFSVALIGI
                     PDKNNSANTVRNHLYTLFGNFTTIKLRDLGNLKKGKSEKEMALALEHVVQELAEKSIP
                     CIVFGGEQWLTTAIFKGITEVQPNVG"
BASE COUNT          616 a          338 c          309 g          559 t
ORIGIN      
        1 ctaactgaat caactctact ccttttgatg ttttcgtttc taaatcagat aaaaagggta
       61 aaataaatcc cttttgaaat tgatctacag attgtacttt ttttagttgt tcgataagaa
      121 ccgaaactgg aatattcggc aaaatctgtg gtagtatggc aagcaactgt ttgtcttcta
      181 ctaatttgct aaattgcaag ggtatttctg catcgttata acaacgaata tcatcaaact
      241 gactatcagg attcatcata gtattatttt cttttgccca aagatacaaa tttttagaaa
      301 aacacgtaca tttgtatgca ataaatacga ctatggcaaa agaaattgaa agaaaatttt
      361 tggtggataa aacaaaatgg caagctgtat caaaaggaat gttgctttct cagggatatt
      421 tgtctgcaac cgaaaacact actgtccgtg tacgcacaac agcggacaaa ggttttttaa
      481 ctatcaaagg aaaaacgcaa aggtttagtc gagatgaata cgaatacata atcccgcttc
      541 aagatgcaca agaaatgcta acgatgtgct ctgacaaact agtaacaaag taccgttaca
      601 cagagaaaca caatgatatg ctttgggaaa tagatgtttt tacagataaa aacgaaggat
      661 taattatagc tgagatagaa cttacaagtg aagaacaaaa attcgtattg cccgactggg
      721 tagaaaaaga ggtaacggga gacatccgct attacaactc atttttggct aaaacgccat
      781 tttctacttg ggaataacat caattccctt attcattaat tttatttact cggttgcagt
      841 gtctgcctcc ttcaaaatca gtagtcagaa aacgctctac catatcctcc gccaaagctt
      901 gcgaaataaa acgtgctggc aatgatataa tgttcgcatc attatgcttg cgagctaata
      961 tggcaatttc atctgtccaa caaagtgctg cacgtacatt tttatgtttg ttggcaacaa
     1021 tacttactcc attgccacta ccacaaatag caataccaaa atcaatttcg tttttagcta
     1081 ctgcttgtgc taatgggtgt atatagtcag gataatcaac actctcttcg gaatgagtac
     1141 caaaatcttt aagagcaaaa aacgtgtgtg caaaatactc tttcaatctt tctttcatct
     1201 catagccagc gtggtcgcta gcaataccta tatacaaggt gtctaatgct ttcatttttg
     1261 ttcgattgtt tgacgataca attatctatt taaaaaaact tgaataaccc aaccatattt
     1321 tactatgggg aatcatattg ttgttcagtt tgcaaaaaat atgtattttt gtttgctctg
     1381 caaagataca tttttgcaca caacaattga ctaaatttac tctaattttt tacaagcatg
     1441 tccgactcta tttccttata ttttaacgac gtattggtaa acccaacatt accatcgtat
     1501 atgtcagaaa atgaatacat tgcatcaaac cttcacacgc aagacttttc ggtagcactt
     1561 attggcatac ctgataaaaa taactctgcc aataccgtcc gcaaccatct gtacacactt
     1621 tttggcaact tcactactat aaaactacgt gatttgggca atctgaagaa aggaaaatca
     1681 gaaaaagaaa tggcattggc attggagcat gtagtacaag agttggcaga aaaatctatt
     1741 ccttgtattg tgtttggcgg agaacaatgg ctcacaactg ctatttttaa aggaataact
     1801 gaggtgcaac ccaatgtagg gg
//
LOCUS       DAOPUR010000323         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_70711_length_1822_cov_3.505942, whole genome shotgun sequence.
ACCESSION   DAOPUR010000323 DAOPUR010000000
VERSION     DAOPUR010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70711_length_1822_cov_3.505942"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PKN90_05970"
     CDS             <1..840
                     /locus_tag="PKN90_05970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNZ85855.1"
                     /translation="LIACAIPSNDDAWQLVLNGKLIKNPLPFASVMTLPATGSEMNRG
                     SVISNKATQEKFAFYAQYPQFSILDPETTFSLPKVQLANGIADTFVHVMEQYLTTPNQ
                     SLLMDRWAEGILQVLVEIAPSVLANNRDYDVMSQFMLAATMALNGFISMGVSQDWATH
                     MIGHELTALHGIDHAQSLAIVLPGTMKVLQTQKAEKILQYGARVWGILQGDDNQRITK
                     SIEATETFFRSLGLATRLSQLQIGDATIMEIEKRFMQRNVHFGEARTVTAQVTTAILN
                     EVR"
     gene            complement(875..1648)
                     /gene="budA"
                     /locus_tag="PKN90_05975"
     CDS             complement(875..1648)
                     /gene="budA"
                     /locus_tag="PKN90_05975"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNZ85856.1"
                     /translation="MKRILTGIAFVVCISSATFATTPDTDVLFQTSTINALLEGVYDG
                     EVKCGELKHYGDFGIGTFQALDGEMIVVDGTIYQVKADGKVYNVADTVKTPFASVTFF
                     DTDVSYTLQKTTNFQQLCTYLDTLIPTKNIFYAFKIEGTFSYLKTRSVPAQQKPYPVL
                     TEVTKNQPVFEFQNIQGVIIGYRIPPFADKINVPGYHFHFLSSDKTVGGHLLEMETNK
                     ANIAIDYTHRFYLSLPEENAFYTTDLSTDKTKELEKAEK"
     gene            complement(1664..>1822)
                     /locus_tag="PKN90_05980"
     CDS             complement(1664..>1822)
                     /locus_tag="PKN90_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNZ85857.1"
                     /translation="FKQQGIFNKKTATSFAENILSKGGSEHPMILYKRFRGQEPTIDA
                     LLKRNGIK"
BASE COUNT          502 a          342 c          375 g          603 t
ORIGIN      
        1 ttgattgctt gtgccattcc gagcaacgat gatgcttggc agctggtgct taacggtaag
       61 ctgataaaaa atcccttgcc ctttgcttcg gttatgacct tacctgctac gggttcggaa
      121 atgaaccgtg gttcagttat ttccaataag gctacgcaag agaaatttgc cttttatgca
      181 cagtatcctc aattttctat tctcgaccca gaaacgacct tttccttacc caaggtacaa
      241 ttggctaatg gcatagcgga tacctttgtg catgtgatgg agcagtatct taccaccccc
      301 aaccaatcgc ttttgatgga tagatgggca gaggggatat tgcaggtatt ggtagagata
      361 gctcctagcg tgcttgccaa caaccgcgat tacgatgtga tgagccagtt tatgttagct
      421 gctactatgg ctctcaatgg ttttataagc atgggtgtat cgcaagactg ggctacacac
      481 atgatagggc acgagcttac agctttgcac ggtatcgatc atgcacagag tttggctatc
      541 gtattgccag gtacgatgaa agtattgcaa acgcaaaaag ctgaaaaaat tttgcaatac
      601 ggtgctcggg tatggggtat tttgcaagga gatgataacc agcgaattac taaatccata
      661 gaagctaccg aaacgttttt ccgctcattg ggattagcca ctcgccttag tcaactccaa
      721 attggtgatg ctacgataat ggaaattgaa aaacgattta tgcagcgtaa tgtgcatttt
      781 ggcgaagcac gtaccgttac agctcaagtt actacagcta ttttgaatga agtgaggtag
      841 gggcagaaat tgtgataata gctatttgat gtatttattt ctctgctttt tccagttctt
      901 ttgttttatc tgttgataaa tctgtggtat aaaaagcatt ttcttcaggc agggataaat
      961 aaaatctgtg tgtataatca attgctatgt ttgccttatt cgtttccatt tccagtaaat
     1021 gtcctccaac tgttttgtcg gatgataaaa aatgaaaatg atagccaggt acgtttattt
     1081 tatcagcaaa aggaggtatt ctgtaaccaa ttattacacc ttgtatattt tgaaactcga
     1141 acacaggctg attttttgta acctctgtta aaaccggata gggcttttgt tgagcaggta
     1201 cactgcgtgt ttttaggtag ctaaaagttc cttctatttt aaaagcataa aagatgtttt
     1261 tggtaggtat cagggtgtca agataggtac atagttgctg aaaattagtc gttttttgta
     1321 aggtatagct cacatctgta tcaaaaaatg taactgatgc aaagggcgtt tttactgtgt
     1381 ctgctacatt atacactttg ccgtctgctt ttacctgata gattgtgcca tctacaacaa
     1441 tcatttctcc atcaagagcc tgaaaagtgc ctatgccaaa atctccatag tgttttaact
     1501 ctccacattt tacttctccg tcatatactc cttctaacaa ggcattgata gttgaggtct
     1561 gaaacagtac atctgtgtcg ggtgttgtgg caaacgtcgc actactaata cacacaacaa
     1621 acgctattcc tgttagtatt cttttcatgg taaacctcat tttttattta attccatttc
     1681 gtttgagtag ggcatcgata gtcggttctt gacctctaaa tcgtttatac aaaatcatag
     1741 gatgttcgga gcctcctttg cttaaaatat tttctgcaaa tgatgtagct gtttttttgt
     1801 taaaaattcc ttgttgttta aa
//
LOCUS       DAOPUR010000324         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_71129_length_1815_cov_3.840909, whole genome shotgun sequence.
ACCESSION   DAOPUR010000324 DAOPUR010000000
VERSION     DAOPUR010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71129_length_1815_cov_3.840909"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1319
                     /locus_tag="PKN90_05985"
     CDS             282..1319
                     /locus_tag="PKN90_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ85858.1"
                     /translation="MKAQITIKDIARELGVSASTVSRALQDHPDISKATIELVKEYAK
                     AHNYKPNAVALSLKKQRSNIIGVVVPEMVHHFFSTVLSGIEDVANSKGYNVLVCQNNE
                     NYEKEVRMISSLISARVSGVIASISKETTNYAHFQELMDDNTPLVFFDRICTGINTDR
                     VIIDDYTGAFSAVEYLIKTGCKRIAFYSAPFTLEISKNRKNGYLDALRKYGIKVDESL
                     VHVCDTREDAIELTQQLLQEPNRPDAFFAINDATASGVLSAVKKAGLRVPEDVSICGF
                     GDGIIASTSDPLLTTVQQNGYQMGVEACNMLLKRIENELSPTQFNHRLIRTSLVVRES
                     TKPLIESPIHY"
     gene            1345..>1815
                     /locus_tag="PKN90_05990"
     CDS             1345..>1815
                     /locus_tag="PKN90_05990"
                     /inference="COORDINATES: protein motif:HMM:NF020653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ85859.1"
                     /translation="MKKTFLIVFLFVTLCLSGYAKSEKVTFERLEPASWWIGMHDTQL
                     QLMIYGKNISSSKVEFLYEGVSLVKRETTDNPNYLFLYLHIDKKTKPGTFDIIFKNGK
                     KQVGKFTYTLQARRENSAQRPSFSPADAIYLLMPDRFCNGNLQNDNVKGYIQGVN"
BASE COUNT          619 a          329 c          325 g          542 t
ORIGIN      
        1 ctatacgaga aacgagaaac actaaacact aaacattaaa cgttaaacac taaactttaa
       61 acgttaaacg ttaaacactt tcaactttcc actttcaact ttccactcaa taatttttcg
      121 catataaaaa cgtgcatttt gtataataaa acactactat actagtattc cactcctaat
      181 cctgcatttt tttttggaaa tattaaaaaa taacgtaatt ttgggcgttg tatttattgc
      241 attgttgtgc aattataaca ccttaaaaat tgatatattg gatgaaagca caaattacca
      301 tcaaagacat agcacgagaa ttgggtgttt ctgcatctac cgtgtcgcgt gcattgcaag
      361 atcatccaga cataagcaaa gctaccattg aattagtaaa agaatacgcc aaagcacaca
      421 actacaaacc caacgcagta gcattaagtt taaaaaaaca acgttctaat atcattggag
      481 tagtggtacc cgaaatggta catcattttt tttctactgt tttatctggt atagaagatg
      541 ttgctaacag caaagggtac aatgtattag tatgtcagaa taacgaaaac tacgaaaaag
      601 aagtacgcat gatttcatcg cttatctcgg ctcgcgtatc tggtgttatt gcttctatat
      661 ctaaagaaac cacaaactat gcccattttc aggaactaat ggatgataat actcctttgg
      721 tgttttttga ccgtatttgt actgggatta atactgatag ggtaattatt gatgattata
      781 cgggtgcttt ttctgctgtt gaatatttaa ttaaaacagg atgcaaacga atagcctttt
      841 atagtgctcc ttttaccctc gaaatttcta agaacagaaa gaatggctat ttagatgcgt
      901 taagaaagta cggcattaag gtggacgaat ccttggtgca tgtatgtgat acacgtgaag
      961 atgctattga gctaacgcaa caacttttgc aagagcccaa cagaccagat gctttttttg
     1021 ctattaacga cgccacagct tcgggagtat tgtctgctgt taaaaaagca ggattgcgtg
     1081 tgcctgagga tgtatcgata tgtggctttg gcgatggtat tattgctagc acttccgacc
     1141 cattgcttac tacggtgcag caaaatggct accaaatggg cgttgaagcc tgcaatatgt
     1201 tgttaaagcg aattgaaaac gaattgagcc ctacccaatt taatcatcgc ttaattcgca
     1261 ccagtttagt agtaagagaa tcgacaaaac ccttaattga aagcccaata cattactaaa
     1321 tacaaataaa aaacctataa ttatatgaaa aaaacattcc ttatcgtttt cttattcgta
     1381 acactctgcc tgagtggata tgccaaatcg gagaaagtaa catttgaacg tttagagcct
     1441 gccagctggt ggattggtat gcacgacaca caattacaat tgatgatata tggcaaaaat
     1501 atatcatcaa gtaaggtcga atttctgtac gaaggtgttt ctttggttaa gagagagaca
     1561 acggataacc caaactattt gtttttgtac ttacatatcg acaaaaaaac taaacccggt
     1621 acatttgata ttattttcaa aaatgggaaa aaacaagtag gaaaatttac ctataccttg
     1681 caagcacgta gagaaaactc ggctcaacga ccaagtttta gccctgcaga tgcaatttat
     1741 ctacttatgc ccgatcgttt ttgtaatggg aacctacaaa atgataacgt taaaggctac
     1801 atacaagggg taaat
//
LOCUS       DAOPUR010000325         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_72042_length_1801_cov_2.893471, whole genome shotgun sequence.
ACCESSION   DAOPUR010000325 DAOPUR010000000
VERSION     DAOPUR010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72042_length_1801_cov_2.893471"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PKN90_05995"
     CDS             <1..753
                     /locus_tag="PKN90_05995"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ85860.1"
                     /translation="KIADLAIWEEKDAIKIDSTYNTIQFYAGIVASNMDSTQLAIKHF
                     KNLIGTSHPQKEAVYEMLYSLYQHANDTVNFISILKEGVELYPESPFFIGNLINYYAT
                     ASKFTEALDYLNEALERSPKNIEYLNVKGTLLIQMGEFDKARAIYTESLALNNQHPNS
                     LFGMGRSWAFEGDLINEKSQDIKDNKLYKKEQERAKEYFINSIKYLEEAKALMSKEDA
                     QYSDLLQNLRVLYLRLGETEKYNKIDAEIKTY"
     gene            complement(816..>1801)
                     /locus_tag="PKN90_06000"
     CDS             complement(816..>1801)
                     /locus_tag="PKN90_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNZ85861.1"
                     /translation="YGYGIPIVEKTLTYNTSLAKTNAIRIDKTPAIMDKLHAIVLSPS
                     SINTFITCPLQFYFSYIENIKQTDSVTESIESNIFGSIFHFVMEKLFVHFKDKMISKE
                     IIDTMIRDTSKIELLISEGFSQFYFKDKTPHVLEGNHYLVAQVISKYVRKLLDIESKR
                     TPFFHITHEYKCEENYYISKYKTFAKIKGSIDRVQIKDGVIMLIDYKTGSGELKYPSI
                     GELFNGNLKDRRKDILQILLYSWLYQQNKKIDSEIECHIIYPRKMFHSDFSTQPFDSQ
                     NNTTLFNHDIKTEFLTHLDNCLVTLFDQEIPFTQTTCIDNCSYCPFNQVCR"
BASE COUNT          622 a          232 c          304 g          643 t
ORIGIN      
        1 aagattgctg atttagctat atgggaagaa aaagatgcga taaaaataga ttccacttat
       61 aatacaattc agttttatgc aggtattgtt gcatctaata tggatagtac tcaactggct
      121 attaagcatt ttaaaaattt aataggtact tcgcatccac aaaaggaggc tgtttacgag
      181 atgctttatt cattgtatca acatgccaat gatacagtaa attttatatc aatattaaaa
      241 gaaggcgttg aattgtatcc tgaaagtcct ttttttatag gtaatttaat taattattat
      301 gctacagcaa gtaaatttac agaagcatta gactatttaa atgaagcctt ggaacgttct
      361 cctaaaaata ttgaatacct taatgtgaaa ggtactttgt tgatacaaat gggtgagttt
      421 gataaagcaa gagctattta tactgaatca ctagcactaa ataatcagca tccaaattcg
      481 ttgtttggaa tgggtagatc atgggctttt gaaggagatt taattaatga gaaatcgcaa
      541 gatattaagg ataataagtt gtataaaaaa gaacaagaaa gagccaaaga atattttatt
      601 aattcaatta aatatttgga ggaagcaaaa gcattgatgt ctaaagaaga tgcacaatat
      661 agcgatttat tgcaaaattt gcgtgtattg tatttgcgtt taggtgagac agaaaaatac
      721 aacaaaatag atgcagaaat caaaacttac taaaatgttg tgatactcct gttttattat
      781 tgtgaggctt ttcgagaaat cggagagcct tttttttacc tacatacttg attaaaagga
      841 caatatgaac aattgtcaat acatgttgtt tgagtaaaag gaatttcttg gtcgaacaac
      901 gttacaaggc aattatccaa atgagttaaa aattctgttt ttatgtcgtg gttaaaaaga
      961 gtagtgttat tttgagaatc aaagggctgt gttgaaaaat cggaatgaaa catttttctc
     1021 ggatatataa tgtggcactc aatttctgaa tcaatttttt tattttgttg ataaagccag
     1081 ctgtagagta gaatttgtag tatatctttt cgtctatctt tcaagtttcc attaaataat
     1141 tccccaattg acgggtattt aagttctcct gaacctgttt tgtaatcaat caacatgata
     1201 actccatcct ttatttgaac tctgtcaata gaacctttta tttttgcgaa tgttttatat
     1261 ttagatatgt aatagttttc ttcgcatttg tattcatggg ttatgtgaaa gaaaggagtt
     1321 cgtttacttt ctatatctaa tagtttccgt acatatttac taataacttg tgccactaaa
     1381 taatgattac cctctaatac atgtggtgtt ttatctttaa aataaaattg tgaaaaccct
     1441 tcacttatta gcagctcaat ttttgatgtg tcacgtatca ttgtgtcaat tatttccttt
     1501 gaaatcattt tatctttaaa atggacaaat aatttttcca taacaaaatg aaatatggac
     1561 ccaaatatat tcgactcaat agattcggta acactatctg tttgttttat gttttcaata
     1621 tatgaaaaat aaaattgtaa tggacaagta atgaatgtat taatgcttga aggcgataaa
     1681 acaatggcat gtaatttatc cattattgcg ggtgttttat caatgcgaat agcattagtt
     1741 ttagctaaag atgtgttata agtaagggtt ttttctacaa taggaattcc gtaaccatag
     1801 c
//
LOCUS       DAOPUR010000326         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_72049_length_1801_cov_2.720504, whole genome shotgun sequence.
ACCESSION   DAOPUR010000326 DAOPUR010000000
VERSION     DAOPUR010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72049_length_1801_cov_2.720504"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1040
                     /locus_tag="PKN90_06005"
                     /pseudo
     CDS             36..1040
                     /locus_tag="PKN90_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
     gene            1125..>1801
                     /locus_tag="PKN90_06010"
     CDS             1125..>1801
                     /locus_tag="PKN90_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNZ85862.1"
                     /translation="MIIYVIFGVVALSSWLIQYRLKSKFEKYSKLALGNGMSGKEVAE
                     KMLRDNGISDVTVISTSGHLTDHYNPTNKTVNLSEAVYNSASVAAAAVAAHECGHAIQ
                     HARSYAFLQMRSKLVPVVSFASNWVQWVLLAGILLMQVMPEIMLAGIVLFAFTTLFSF
                     ITLPVEINASSRALAWLSNAGITNAFNHNEAKDALKWAAYTYVVAALGSLATLVYYIF
                     IFLGRRN"
BASE COUNT          571 a          319 c          385 g          526 t
ORIGIN      
        1 tatcttcaaa aagaattgcc ctataactca ttcctggata tgatttatcc caaaaattag
       61 ttatttctaa aaaagcacac ctcattttac ctactcaccg cttaatagac gccgcacaag
      121 aagcacaaaa gggtgatgca aaaattggtt ctacgctcaa aggaattgga cctacttatg
      181 ctgacaaaat tagccgaacc ggtcttcggg tgggagattt actacataat tttaccgaaa
      241 aatatgcaaa aaaaatagct gaccatacgc gtttgcttga ccaaatgggg ttctcttatt
      301 cgttagatgg gctagaggaa gaatggatgg aggcaacaaa aaccctcctt cgttttcagt
      361 taattgatag cgaacatgtt gtaaacgaat taatcgctga taataaacgt attttagctg
      421 aaggtgcaca aggcacgatg cttgatattg aatttggttc gtatccgttt gtaacatcat
      481 caaacactgt gtcagctgga gtttgtacag gactaggcgt tcctcctacc caaattggag
      541 aagtgtacgg cattatgaaa gcatattgta cgcgcgtggg cagtggacct ttccctacgg
      601 aattgtttaa cgaagaggga gaagaaatgc gtaaaaaagg gaatgagttt ggttcaacta
      661 cgggcagagc tcgccgttgt ggctggatag atttagtagc actgaaatac gctattatga
      721 ttaatggtgt aacgcaatta attatgatga aaagcgatgt gctcgaccaa tttgacgtaa
      781 taaaagcatg tgtgggctat aaaattgatg gcgaagaata tgattatttc ccttacgaaa
      841 taaacgccaa cttagaacct atgtatgtag aattgccagg atggaaaacg gatatgtcga
      901 aaatgcagaa agagagtcaa tttccacaag cgtttaatga ttatctcgat ttcttagaag
      961 acgaattgga agtacccatt cgcattgtat cggtaggacc cgatagagaa caaacaattt
     1021 tacgttacat taacgaataa cggggtgttt ttaacactga caaacgcaaa attggcagta
     1081 tttttgatgt atgtaattgt gtataaccat taacgtaatt ttgtatgata atttatgtaa
     1141 tttttggagt agtagccctg agtagttggc ttatccaata ccgtttgaaa tcaaagtttg
     1201 aaaaatactc taaacttgct ttaggcaatg gtatgagtgg aaaagaagtg gcagaaaaaa
     1261 tgcttcggga taatggcatc agcgatgtaa cagttatttc tacaagcgga catttaacag
     1321 accattacaa ccccacgaat aagacagtca acttaagtga agcagtatac aactctgcca
     1381 gtgtggcagc agcagcagta gccgctcacg aatgcggaca cgccatacaa cacgctcgct
     1441 cgtatgcgtt tttgcaaatg cgttctaaat tagtgccagt agttagtttt gcttctaact
     1501 gggtacaatg ggttctttta gcaggtattc tacttatgca agttatgcca gagattatgt
     1561 tagctggtat tgtattattt gcatttacta ccttatttag ttttattact ctacctgtag
     1621 aaattaatgc aagcagcaga gccttagctt ggttgtcaaa cgcaggtatt accaatgcct
     1681 ttaaccacaa cgaagcaaaa gatgccctca aatgggctgc atatacgtat gtagtagctg
     1741 ccttgggttc gttagcaacc ttggtatatt acatctttat atttttgggc agacgtaatt
     1801 a
//
LOCUS       DAOPUR010000327         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_72583_length_1793_cov_2.204833, whole genome shotgun sequence.
ACCESSION   DAOPUR010000327 DAOPUR010000000
VERSION     DAOPUR010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72583_length_1793_cov_2.204833"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /gene="xseA"
                     /locus_tag="PKN90_06015"
     CDS             complement(<1..1179)
                     /gene="xseA"
                     /locus_tag="PKN90_06015"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNZ85863.1"
                     /translation="MPPHAHIRLVELNSFVKKLIADNTQPQWITAEISEVNEHYSGHC
                     YLELIEKDDADEHIIAKAKAVIWSFTYRMIKPYFETTTHERLMAGMKVLVKVEVSFHE
                     AYGYSLVIKDIDPQYTLGDMARKRALIIQQLYAEGVMDLNKEIDLPLLVQRIAVISSP
                     GAAGYTDFCNQLHGNAFGFVFYHRLFAAVMQGTETEASIINALEAVYEHKELFDVVVI
                     IRGGGATSDLSWFDNYNIAYHCTQFPLPILSGIGHDKDVSVVDMVAHTSLKTPTAVAQ
                     FLVDCMQQTESNLHTVKEEIINYTEQALESWQQRLQLVTLQLPNAVKRKVEQAYYTLT
                     SSIQHLQHRSKQNLTQKAYTLDKHQQYVQLVSPIRIFEKGYTLTLKNGKPIHNQILAK
                     G"
     gene            complement(1195..>1793)
                     /locus_tag="PKN90_06020"
     CDS             complement(1195..>1793)
                     /locus_tag="PKN90_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85864.1"
                     /translation="GKLKAIQQFATNDTVKVEYFAGDGSQLAKGIFVKGQKQGIWEYF
                     SKENRLVMVENYTEGLKQGESLVFSESGMVIDRMQYENNVLHGKRIQYHPYGNILASY
                     FYKNGLLDGPFESYYETGQKDEVGYYVANKKHGEWKTYNDDGSFTSVEYKNGKATNQT
                     ELDKKLQETMDKNEQGDPTIKDPADYLDQPDAYLNLFR"
BASE COUNT          513 a          391 c          293 g          596 t
ORIGIN      
        1 tccttttgcc agtatttgat tgtgaatcgg tttgccattc tttagtgtaa gcgtatatcc
       61 tttttcaaat atgcgtatag gcgaaaccaa ttgtacatat tgctggtgtt tatctaaggt
      121 gtatgctttt tgcgtaagat tctgtttact acgatgctgt aagtgttgta tggaagatgt
      181 taacgtataa taagcttgtt ctacttttcg ttttacagcg ttgggcaact gcaatgtaac
      241 tagttgcaat cgttgttgcc aactctcaag ggcttgttct gtatagttta ttatttcttc
      301 ttttacagta tgtaaattac tttcggtctg ttgcatacaa tccaccaaaa attgagcaac
      361 agccgtgggc gttttcaaac tggtatgtgc caccatatcc accaccgaca catctttatc
      421 atgaccaatc ccactcaaaa taggcaacgg aaactgtgtg caatgatagg caatgttata
      481 attatcaaac caactcaaat cagaagtagc tccaccgcca cgtatgatga ccaccacgtc
      541 aaacaattct ttgtgctcat acacagcttc tagggcattg ataattgaag cctctgtttc
      601 agtgccttgc ataacagcag caaacaatcg atgataaaac acaaacccaa acgcattgcc
      661 atgtaattga ttacaaaaat ctgtataacc tgcagcacca ggcgaagaaa taacagcaat
      721 acgttgcaca agtaatggca aatcaatctc tttgtttaaa tccatcaccc cttcggcata
      781 caattgttga ataatcaagg ctcgcttacg agccatatcg cctaaggtgt attgaggatc
      841 aatatctttt attaccaaac tatatccata ggcttcatga aaacttactt ctacttttac
      901 caacaccttc atcccagcca taaggcgttc gtgtgtggtt gtctcaaaat aaggttttat
      961 catgcggtag gtaaacgacc aaattaccgc tttggctttg gcaatgatat gctcatcagc
     1021 atcatctttt tctatcaact cgaggtagca atgacctgaa taatgttcgt ttacttcact
     1081 aatttctgca gtaatccact gaggttgtgt attgtcagca atcagttttt taacaaaact
     1141 attcagttca actaatcgta tgtgtgcgtg tggaggcata acataaccga cttattagcg
     1201 aaacaaatta agatacgcat ctggttggtc aagataatca gcaggatctt ttattgttgg
     1261 atcgccttgt tcgttcttat ccattgtctc ttgcagtttt ttatccagtt ctgtctgatt
     1321 agtagctttc ccatttttat attctacaga ggtaaacgaa ccatcatcat tgtatgtttt
     1381 ccattctcca tgttttttat tggctacata atagcctact tcgtcttttt gaccggtttc
     1441 ataatatgac tcaaaaggac catctaacaa tccatttttg taaaaatagc ttgccaaaat
     1501 attgccatac ggatgatatt gaatacgttt tccatgcaac acattatttt cgtactgcat
     1561 tcggtcaatt accatacccg actccgaaaa aaccaacgac tctccttgct tcagtccttc
     1621 tgtataattc tctaccatca ccaagcggtt ttctttagaa aaatactccc aaataccttg
     1681 tttttgtcct tttacaaaaa ttcctttcgc caattgagaa ccatcaccag caaaatactc
     1741 cactttcaca gtatcattcg tggcaaattg ttggatagct ttcagtttcc cac
//
LOCUS       DAOPUR010000328         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_73021_length_1786_cov_2.415367, whole genome shotgun sequence.
ACCESSION   DAOPUR010000328 DAOPUR010000000
VERSION     DAOPUR010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73021_length_1786_cov_2.415367"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKN90_06025"
     CDS             <1..427
                     /locus_tag="PKN90_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85865.1"
                     /translation="TITIFAYIKKKKLYLLDGFLFLIAGSVGSLLLFLALFSKHPFTD
                     NNFNIVWLNPFWFVPFVLFIIPIPRKKDVLIAFYIAVSIVLTIFMLSIWAIPQYFNPA
                     FIPLVLVLLTRVVSYVFYWGYKKDKTGSYYKRKKRTSNK"
     gene            complement(486..686)
                     /locus_tag="PKN90_06030"
     CDS             complement(486..686)
                     /locus_tag="PKN90_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNZ85866.1"
                     /translation="MKHVVGTNAGVVWGALAEANGTLEFKELKKLTKLKEKDLYLAFG
                     WLLKEGKLVFREEEKDLYISLV"
     gene            complement(783..>1786)
                     /locus_tag="PKN90_06035"
     CDS             complement(783..>1786)
                     /locus_tag="PKN90_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ85867.1"
                     /translation="QQITVVELAKPYYILLIISLFPFLMFSVYKQFVEGLGNTAVAMY
                     ITIIANIINIVFNYLLIFGKFGFPAWGLVGAGIATLLSRIVMWILFVVVVRYNLTWRS
                     YVQSFSWSLFNTKTIKRLASVGFPIGGQMFLEISAFGISTIMVGWLGDVSLAAHQIAQ
                     IMAHVSFMGILGLAAAGTVRVSHEYGHKNIYNLQMSITASRHLVLVMTCFTGIILLSM
                     RNYIPLIFTTDSTVIEIASTLIVLVGLFQISDGMQAVGAGVLRGLTDVNVMVRYAFFA
                     YICINIPVAYLLAFVVGLREQGIWIGFIFGLSCAAILFYLRYKKKIKLLQTTLNKIS"
BASE COUNT          656 a          329 c          236 g          565 t
ORIGIN      
        1 aactataacc atattcgcat atataaaaaa gaaaaaatta tacctattag atggctttct
       61 atttttaatt gcaggctctg tggggtcatt attgttgttc ttagcattat tctcaaaaca
      121 tccttttacc gataacaact tcaatattgt atggttgaat cccttttggt ttgttccctt
      181 tgtattgttc attataccta ttccgcggaa aaaagatgta ttgattgctt tttatattgc
      241 tgtgagcatt gtacttacca tatttatgct atctatatgg gcaattcccc aatactttaa
      301 tccagccttc atccctcttg tattagtatt actcacaaga gttgtttcgt atgtctttta
      361 ttggggttat aaaaaagata aaacaggaag ttattataaa cgaaaaaaaa gaacatcaaa
      421 caaataaaca tcaaagaggc tatctattaa atagatagcc tctttgatat ataacagata
      481 agaatctata ccaaagaaat atatagatct ttttcttctt ccctaaacac caattttcct
      541 tctttaagca accaaccaaa agctagatat agatcttttt ctttaagttt tgttagcttt
      601 tttaattcct taaactcaag tgtaccatta gcttctgcta aagcacccca aacgacacct
      661 gcatttgttc ctactacgtg tttcataact tatacatttt atgtggttag acaatagtac
      721 aaaagtaacc taaaatattg caaacacatt agccaatgtg ttgatttaat aaaaacttaa
      781 aactacgaaa ttttatttag ggtagtttgt aacaatttta tcttcttttt gtaacgtaaa
      841 taaaataata tagctgcaca actcaagcca aatataaaac ctatccaaat accttgttct
      901 ctgagtccta ctacaaaagc caatagataa gcaactggaa tattaataca tatataagca
      961 aaaaaagcat atcttaccat tacatttaca tctgttagtc cacgcaaaac gcctgctcct
     1021 actgcttgca taccatctga tatttgaaac aatccaacta atacaattaa tgtagaagca
     1081 atctcaatta cagtagaatc tgtggtaaag attaatggaa tatagtttcg cattgatagt
     1141 aaaattattc ccgtaaaaca agtcataaca agtaccaaat gacgtgaggc agtaattgac
     1201 atttgtagat tatagatatt tttgtgtcca tactcatgac taacacgcac tgttccagcg
     1261 gcagctaatc ctaatatccc cataaaagaa acatgagcca taatttgtgc aatctgatgt
     1321 gcagctaaag aaacatcacc tagccatcca accataatag tggatatccc aaaggcggaa
     1381 atttctaaaa acatctgacc accaatcgga aacccaacag aagcaagacg tttaatcgtt
     1441 tttgtattaa acaacgacca tgaaaaagat tgtacataac tacgccaagt aagattatat
     1501 cggacaacaa caacaaagag tatccacatc acaatacggg ataataatgt agcaatacct
     1561 gctcccacta aaccccaagc tggaaatcca aatttaccaa atattaaaag ataattaaat
     1621 acaatattta ttatattggc gataatggtt atatacatag caacagctgt atttcctaag
     1681 ccctctacaa attgtttgta aacagaaaac attaaaaaag ggaataaact tataattagt
     1741 aagatataat atggttttgc taattcaact acagtaattt gttgac
//
LOCUS       DAOPUR010000329         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_73134_length_1784_cov_2.646617, whole genome shotgun sequence.
ACCESSION   DAOPUR010000329 DAOPUR010000000
VERSION     DAOPUR010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73134_length_1784_cov_2.646617"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKN90_06040"
     CDS             complement(<1..470)
                     /locus_tag="PKN90_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ85868.1"
                     /translation="MQQTEWFPIVTETGERIGKATRKECHSGSFLLHPVVHLHVFNAK
                     KQLFLQQRAANKDIQPNKWDTAVGGHVDDGESIQIALVREAYEELKIKDFSANFLFQY
                     PFRSQVEHELVHSFWVQYDFPIQANLEEIQDARFWEIDEIKNSLGKQLFTPNFE"
     gene            571..>1784
                     /locus_tag="PKN90_06045"
     CDS             571..>1784
                     /locus_tag="PKN90_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ85869.1"
                     /translation="MNYHRIFRTYFLYKLIFKYVRCIHAHILHRSYTVYNAENIPTGK
                     PFIMVANHQHGILDALALLFMIKDGRYPVFMARADVFKNKYVAQLLVFFRLLPAFRMQ
                     DVGKNRVLENEKVFATTAQLLAQHNGILALFPEGGHQERKQLGKLKKGFARIAFQTAS
                     CANFETEIFVLPVGIHHIGGLFQSKVSVCVGKPISVLTFQELYMQEPEKAQQQLLEQT
                     ARAMSSNMLDIKETTYYNEYLVLCTIARIYRKNNAAIQERYATDLAIEQAVVSRVELL
                     KVHQETTYVELMKLTKNMDELIVSLHLSYELVCEKKHSIAKRGMHVFITILFIPIQIY
                     AFVHQIIPFLLTSVCSRNAGDELLKVSLQLALGALFIFPLWNGFLVFILFLLEVPFSW
                     LVCYGISLLFSI"
BASE COUNT          535 a          282 c          339 g          628 t
ORIGIN      
        1 tcttcaaagt ttggagtaaa caattgtttc cccaaagaat ttttaatctc atctatctcc
       61 caaaacctag catcttgaat ttcttctaag ttggcttgaa tgggaaaatc gtattgaacc
      121 caaaagctat gtaccaattc gtgttctact tgtgagcgaa agggatactg gaagagaaag
      181 tttgccgaaa agtcttttat ttttaattct tcatacgctt cgcgaactaa ggctatctgt
      241 atgctttcgc catcatctac atgcccaccc acggcagtgt cccatttatt tggctgaatg
      301 tctttattgg ctgcacgttg ttgcaaaaat aattgtttct tggcgttaaa tacatgaagg
      361 tgtaccactg gatgaaggag aaaagaccct gaatgacact cttttcgggt tgctttacca
      421 attctttcgc cagtttcggt tactatagga aaccattctg tttgttgcat aacattattt
      481 ttgtacaaaa gtagaaaata tttcttattt taagcgtata cgagttgtct gaatactgta
      541 tctttgtttt tttattagaa caattaacga atgaattatc atcgtatctt tcgtacttat
      601 tttttataca aactgatttt taaatacgtt cggtgtatac atgctcatat attgcatcgt
      661 tcatatactg tatataatgc agaaaatata cctactggca aaccatttat tatggtagcc
      721 aatcatcaac atggaatttt ggatgctttg gcattattat ttatgattaa agatggtaga
      781 tatccggtgt ttatggcacg tgctgatgtg tttaagaata agtatgttgc acaattgtta
      841 gtcttttttc gtttactacc agcctttcgt atgcaagatg taggtaaaaa tagggtgctt
      901 gaaaatgaaa aagtgtttgc aactacggct cagttacttg ctcagcataa tggcatttta
      961 gcactttttc ctgaaggggg gcatcaagaa aggaaacaat taggaaaact gaaaaaagga
     1021 tttgctcgga tagcctttca aacagcctct tgtgccaatt ttgaaacaga aatttttgta
     1081 ttacccgtgg gtattcatca tataggagga ttgtttcaga gtaaggtttc ggtgtgtgtt
     1141 ggcaaaccca tttctgttct tacttttcaa gaactctata tgcaagagcc agaaaaagca
     1201 caacagcagt tgcttgaaca aacagcaaga gctatgagta gtaatatgct agacataaaa
     1261 gaaacaacat attacaacga atatttggtg ctgtgtacga tagctcgtat atatcgaaaa
     1321 aataatgcag ctattcaaga gcgttatgct actgatttgg ctatagaaca agctgttgta
     1381 agtagggtag aattgcttaa agtacatcaa gaaacaacat acgtagagtt gatgaagttg
     1441 actaaaaaca tggatgaact aattgtaagt ttgcatttgt catacgaatt ggtgtgtgaa
     1501 aaaaaacact ctattgcgaa gcgaggcatg catgttttta ttacgatact atttattccc
     1561 atacagatat atgcatttgt tcatcagatt atcccttttt tattaactag tgtatgtagt
     1621 agaaatgcgg gagatgagtt gctaaaagtt tctttgcaat tagctctagg agctttattt
     1681 atttttcctt tatggaatgg atttttagtg tttattctat ttttactaga agttccattt
     1741 tcttggttgg tttgttatgg catctctctt ctttttagta tttg
//
LOCUS       DAOPUR010000330         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_73175_length_1783_cov_3.564815, whole genome shotgun sequence.
ACCESSION   DAOPUR010000330 DAOPUR010000000
VERSION     DAOPUR010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73175_length_1783_cov_3.564815"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="rsgA"
                     /locus_tag="PKN90_06050"
     CDS             <1..839
                     /gene="rsgA"
                     /locus_tag="PKN90_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNZ85870.1"
                     /translation="NFRLKGIRSTNPIAIGDNVLVDLQTNGHSFIYDICDRKNYIVRK
                     SSNLSKHSHIIGANIDQAILVVTVNYPETSTGFIDRFLVSADAYHVPVIIVFNKVDLY
                     IENERNYLDALKQLYQHIGYRCIETSVVSMQGIEELKECLRGNISLFSGNSGVGKSSL
                     IHQIDPTLDVKIGLISEMHNKGMHTTTNSHMYTLSGGYIIDTPGIKGFGIVDIKKHEV
                     AHYFRDIFEYSHQCKYNNCLHQQEPDCAVIKAVGEFRISQSRYQSYLSILQDMTEDAK
                     YR"
     gene            917..>1783
                     /locus_tag="PKN90_06055"
     CDS             917..>1783
                     /locus_tag="PKN90_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ85871.1"
                     /translation="MTSIYEVRQRFKLLFSSLAILIAISSFVVTNSLVQKLSDEEKSK
                     MQIWANATKELINMDEYCDISFVQQVIEANTTIPVILVTQEDSVISYKNIPERLQESR
                     KLQQLVEEYKKSNTPIEIVFSDTERQYLYYGNSLLLIQLSYFPFVQLIAVFIFLVFVI
                     WALLSTKKAEQNEVWVGLSKETAHQLGTPISSLMAWTEIFKSKYIEDSMFGEMEKDVN
                     RLRTIAERFSQIGSKTDLEIVNLNQVIHNAVDYLRTRISKKITLSIQENEVLYCELNV
                     SLFEWVIENLCKN"
BASE COUNT          616 a          234 c          344 g          589 t
ORIGIN      
        1 ggaattttag gctgaaggga attagaagta ctaatcctat agccattggg gataatgtgc
       61 tagttgattt acaaacaaat ggtcattcat ttatttatga tatatgcgat cgaaagaatt
      121 atattgtgcg aaagtcatca aatttgtcga aacattcgca tattattggg gcaaatattg
      181 accaagctat attagtggtt acggtaaatt atccagaaac ttctacaggt tttattgata
      241 gatttttggt gagtgccgat gcgtaccatg tgcctgtgat aattgtgttt aataaggttg
      301 atttgtatat agaaaatgag cgtaattatt tggatgcttt aaaacagttg tatcaacata
      361 ttgggtatcg ttgtatagaa acttcagtag taagtatgca gggtatcgaa gagttgaaag
      421 agtgtttaag aggaaatatt tctctttttt ctggtaattc gggtgttggt aaatcaagtt
      481 tgatacacca aattgaccct acattagatg ttaaaattgg gcttatctca gaaatgcata
      541 ataagggaat gcatacaaca actaattccc atatgtatac attgtcagga ggttacatta
      601 ttgatacgcc gggtattaag ggctttggta ttgtagatat aaaaaagcac gaggtagcac
      661 attattttag agatattttt gaatactcgc atcaatgcaa atataataat tgtttacacc
      721 aacaagagcc agattgtgct gttataaaag ctgtaggaga atttcgtata agtcaatctc
      781 gataccagag ttatttgagt attttacagg atatgactga agatgcgaaa tacagataaa
      841 cggatatact ttttttatat aatatacgtt atatgtattg gttgtttaca ttaacgtaca
      901 caaaatatac attcttatga cttccattta cgaagtaagg caacgcttta aactgctttt
      961 ttcaagtctt gcgatattga ttgcaataag ctcgtttgtt gtaactaaca gtttggtgca
     1021 aaagctttca gatgaagaaa agtctaaaat gcaaatatgg gcaaatgcta cgaaagagtt
     1081 gattaatatg gatgagtatt gtgatatttc atttgtacaa caagtaatag aggcaaatac
     1141 tactatccca gttatacttg ttacacaaga ggacagtgtt atttcgtata aaaatattcc
     1201 agagcgtttg caggaatcta gaaaattaca gcaattagtt gaagaatata aaaaaagcaa
     1261 tacgcctatc gaaattgtat ttagtgatac agaacgacaa tatttgtact atggaaattc
     1321 actgttactt attcaattgt cgtattttcc ttttgttcaa ttaatagcag tatttatatt
     1381 tttagtattt gttatatggg ctcttttaag caccaaaaaa gcagaacaaa atgaagtatg
     1441 ggtgggattg tctaaagaaa cggcacatca actagggact ccaatatcct ctttaatggc
     1501 atggacagaa atttttaagt cgaaatacat tgaagattcg atgtttggtg aaatggaaaa
     1561 ggatgtaaat agattacgta ccattgcaga acgtttctcg caaataggct ccaaaacaga
     1621 tttggaaata gtgaacttaa atcaagttat acacaatgct gtagattatt tacgaacacg
     1681 catctccaaa aaaatcactc tttctataca agagaatgaa gtactctatt gtgaattgaa
     1741 tgtatcatta tttgagtggg tgattgaaaa tttgtgcaag aat
//
LOCUS       DAOPUR010000331         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_73308_length_1781_cov_3.243337, whole genome shotgun sequence.
ACCESSION   DAOPUR010000331 DAOPUR010000000
VERSION     DAOPUR010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73308_length_1781_cov_3.243337"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1781)
                     /locus_tag="PKN90_06060"
     CDS             complement(<1..>1781)
                     /locus_tag="PKN90_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HNZ85872.1"
                     /translation="IYRDKEIKAELASMFPYGEWLSKNRIKLDQISSARHPKNDVPNF
                     QQLLRLFGYHKEDVEAILTPMAQDGNEPVSSMGNDTPLAPFSSKQQLFYNYFRQQFAQ
                     VTNPPIDPIREELVMATSSYMGVINHNLLQPTPELCKMVQISSPILTNQHIDILRNLS
                     YKGFRTITLDATYTLQNGVDGLEQAINNLCQAAEKAVDEDYNYIILSDKAVNGTQAPI
                     PSLLSVAAVHNYLIDKRKRVQTALVVETADAREVMHMALLIGYGASSINPYMAYAVIN
                     DLVKSKTIQLDYHTAEKNYIKSLNKGLKKVISKMGISTIRSYRGAQLFEALGISSELL
                     SKYLHGTITKLEGIGLEDIHRDIVACHQQAFASELELLSNSGQYAYRRNGEYHAWNPE
                     TIAKLQIATRTGDYAKFKEYTRHVDEKEQTVFIRDLIDYKRNPIDISLVEPIENITRR
                     FVTGAMSYGSISTEAHEALAIAMNTLHGKSNTGEGGEDPKRFLPRPDGTSARSAIKQV
                     ASGRFGVTTNYLVNADEIQIKIAQGAKPGEGGQLPGFKVDEIIAKTRHSIPGISLISP
                     PPHHDIYSIEDLAQLIFDLRSVNPAAR"
BASE COUNT          515 a          333 c          318 g          615 t
ORIGIN      
        1 tacgtgcagc tggattcaca ctacgtaagt caaatataag ttgagccaaa tcttcgatag
       61 aataaatatc gtgatgaggt gggggagata tgagcgaaat accaggaatt gaatgtcttg
      121 ttttcgcaat aatctcgtca actttaaaac caggtaactg accaccttct ccaggtttag
      181 ccccttgtgc tatttttatc tgaatttcgt cagcatttac caaataatta gtagttacac
      241 cgaagcgtcc agaagctact tgtttgatag cactacgagc agaagtgcca tcgggtcgtg
      301 gcaaaaatct tttaggatcc tctcctcctt ctcctgtatt actttttccg tgcaatgtat
      361 tcatggcaat agccaaagcc tcgtgagctt ccgtactaat tgagccatac gacatagccc
      421 cagttacaaa acgtctagta atattttcga taggttctac caaagaaata tctataggat
      481 tacgtttata atcaatcaag tcacgaataa acactgtttg ttctttttcg tccacatgac
      541 gcgtatattc cttaaattta gcataatcac ctgtgcgggt tgctatttgt aacttggcaa
      601 tggtttcagg attccaagcg tggtattctc cattgcgtct gtatgcgtat tgaccactat
      661 tagaaagtaa ttctaattca gatgcaaaag cttgttgatg acacgctacg atatctcgat
      721 gaatatcttc taatccaatg ccttctaatt ttgtgatagt tccatgtaga tatttgctaa
      781 gcaactcaga cgaaatacct aaagcttcaa acaattgtgc tccacggtaa ctacgaattg
      841 tagaaatacc cattttagaa attacttttt tcagcccttt atttaaagac tttatgtaat
      901 ttttttcagc tgtatggtaa tctaattgaa tagttttgct ctttaccaaa tcgtttatta
      961 cggcatacgc catatatgga ttaatagagc ttgctccata gccaattaat aaagccatgt
     1021 gcataacttc acgtgcatct gctgtttcta caactaaagc tgtttgtacg cgtttgcgtt
     1081 tgtctattaa ataattatgt acagcagcta ctgataataa tgatggtata ggtgcttggg
     1141 taccgtttac tgctttatca gaaagtataa tataattgta atcttcatca accgcttttt
     1201 cggctgcttg acacaaattg ttaatagcct gttctagtcc atctactcca ttttgcaagg
     1261 tgtatgtggc atctaacgta atggttctaa aacctttata cgataaatta cgtaaaatgt
     1321 caatatgttg attcgttaga attggactac tgatttgtac cattttacat aattcaggtg
     1381 ttggttgcaa taagttatgg ttaattactc ccatataact gctagtagcc attaccagtt
     1441 cttccctaat agggtcaata ggagggttgg ttacttgtgc aaattgttgt ctgaaataat
     1501 tgtaaaaaag ttgttgctta gacgaaaaag gagctagtgg cgtatcattt cccatagaac
     1561 tgacaggttc atttccatct tgtgccatag gagttaatat tgcttccaca tcttctttgt
     1621 gatagccaaa tagacgcaat aattgttgaa aatttggtac atcatttttt ggatgacgcg
     1681 cagaacttat ttggtctagt tttattctat ttttagatag ccattcacca tatggaaaca
     1741 tagatgccaa ttcagcttta atttctttgt ctcggtaaat t
//
LOCUS       DAOPUR010000332         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_73864_length_1773_cov_2.714203, whole genome shotgun sequence.
ACCESSION   DAOPUR010000332 DAOPUR010000000
VERSION     DAOPUR010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73864_length_1773_cov_2.714203"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PKN90_06065"
     CDS             complement(<1..116)
                     /locus_tag="PKN90_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb6"
                     /protein_id="HNZ85873.1"
                     /translation="MDYKKTNAPKSTISRDTTSLCENTGNIYETVKIIGKRA"
     gene            complement(131..946)
                     /gene="bamD"
                     /locus_tag="PKN90_06070"
     CDS             complement(131..946)
                     /gene="bamD"
                     /locus_tag="PKN90_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNZ85874.1"
                     /translation="MHKFYILFISVMVLLLSSCSNYQKLLKNPDPAIKYEAALEYFAK
                     KDYMRSLTLLQDITPVYRGTEKAENILYLIASSNFHQRDYISARHYYSVYISSYPRGK
                     YIEEVKYMNALSFYKESPEPKLDQTASSKAIELFNDFLLEYPKTDKLVQIQLLVSELQ
                     EKLAERELISATLYYNIGNYRGNNYYRSAIVTAENALKDYPQSKFKEDFSILVVRSKY
                     QEAIQSVENKLYERSVVALDECIYFLQEYPTSNYIKEVQQLKLELEKIIIRFT"
     gene            complement(1031..>1773)
                     /locus_tag="PKN90_06075"
     CDS             complement(1031..>1773)
                     /locus_tag="PKN90_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ85875.1"
                     /translation="ILLFTDADCHTVSKDWIEKIVRNFDDNTEIVLGYGAYRKTKGFV
                     NHLIGYDTLFIAIQYLSFALAKKTYMGVGRNMAYKKSLFYKNKGFASHLNIQSGDDDL
                     FIIEASSKTNTKIEIHPNSITVSEQNTSLKSWFNQKQRHISTSKYYKWRSKFILGTEV
                     FSRGLFYISTIALMLALPSHTTYIVVGSFIFVRYIAQITIINYAATILGERKYISSIP
                     LFDILLPLFNLYVWCRSLIHRKQHYKWK"
BASE COUNT          555 a          267 c          271 g          680 t
ORIGIN      
        1 tttgctcttt ttccaataat tttaactgtt tcataaatgt ttccagtgtt ttcacacaag
       61 cttgtggtgt ctctagaaat cgtacttttg ggtgcattcg tttttttgta gtccatacgt
      121 tttagctaaa ttatgtaaat cttattatta ttttttcaag ttccaattta agttgctgaa
      181 cttcttttat ataattactt gtgggatatt cttgtaaaaa ataaatgcat tcatctaaag
      241 ccacaacact tcgttcgtaa agcttatttt ctacactttg aatggcttct tggtattttg
      301 accgtacaac taaaatagaa aaatcttcct taaattttga ttgtgggtaa tctttcaatg
      361 cattttctgc ggtaactatg gctgaacgat agtagttatt gccacgataa ttacctatat
      421 tataatacaa ggtggcacta ataagttctc tttcagctaa tttttcttga agttcactaa
      481 ctaaaagttg aatctgtact aacttatccg ttttcgggta ttctagtaaa aaatcgttga
      541 acaattcaat ggctttacta ctcgcagttt gatctaattt aggttctgga gattctttat
      601 agaaactcaa tgcattcata tacttaactt cttctatata ttttcctcta ggatatgacg
      661 aaatatatac agaataatag tgtcgtgcag aaatataatc tcgttgatga aaattactag
      721 atgcaattaa atacaagata ttttcagctt tttctgtgcc tcgatatact ggagttatat
      781 cttgtaatag ggttaaagaa cgcatataat ccttctttgc aaaatactct aaggctgcct
      841 catatttaat agctggatct gggtttttga gtaatttttg gtaattactg catgatgaca
      901 atagtagcac cattacactt ataaaaagga tataaaattt atgcataagt ctttttgatg
      961 aataatctgc aaacttacat atttgtactg aatgcagaaa ataaaaaacg tttttttaac
     1021 aagaattcta ttatttccat ttataatgtt gttttctatg tattaacgac ctgcaccata
     1081 cgtataggtt aaataatggt agtaatatat caaataatgg gatactactg atgtattttc
     1141 tttctcccaa aatagttgca gcatagttaa tgattgttat ttgtgctata tagcgtacga
     1201 atatgaacga cccaactaca atatatgttg tatgagaggg taatgcaagc atcagggcta
     1261 ttgtgctgat ataaaaaagt ccacgtgaaa aaacttctgt tcctaagatg aatttactcc
     1321 tccatttgta gtattttgat gtggatatat gtcgttgttt ttggttaaac cagcttttta
     1381 aagatgtatt ttgttcagat acggtaatac tgttgggatg tatctcaatt ttcgtatttg
     1441 tcttggaaga agcttcaata ataaataaat catcatcgcc agattgaata ttgagatgag
     1501 atgcaaaccc tttgttttta taaaataagg attttttata tgccatattt ctaccaactc
     1561 ccatataggt tttttttgcc agagcaaaac ttaaatattg tattgctata aataatgtgt
     1621 cgtatcctat gaggtgattt acaaatcctt ttgttttacg ataagctccg tatccgagta
     1681 caatttctgt attatcatca aagttgcgta ctattttttc aatccaatct ttactaacag
     1741 tatgacaatc agcatctgta aataataaaa ttt
//
LOCUS       DAOPUR010000333         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_74033_length_1770_cov_3.993003, whole genome shotgun sequence.
ACCESSION   DAOPUR010000333 DAOPUR010000000
VERSION     DAOPUR010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74033_length_1770_cov_3.993003"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1770
                     /locus_tag="PKN90_06080"
     CDS             <1..>1770
                     /locus_tag="PKN90_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ85876.1"
                     /translation="IFVRDVAQVRDTIKDVTLEEKINGKDGARLIIMKQSGANTVEIA
                     KKVREQIAQVQKTLPPDIHFEIIYDSSELIQNAVFGLSEAIMYALIFVVFVVLFFLGK
                     WRATFIISLTIPISLIVSFIYLYYTGSSLNIISLSSLSIAIGMVVDDAIVVLENINIH
                     IERGSSPREAAIYATNEVWVSVIATTLVIVAVFLPLTLVGGITGVMFKELGWIVTIVV
                     CVSTLVAITLTPSLSAMMLTIKKKTENSKTNRFSYQNTVVKWLDKVDELYANILRWCL
                     RNKAITLTLAVLIFVGSLVPVFMGAIGTDFMAQNDVGRLTITIELQRGTRLEEASKTA
                     RIIESRIAKIAPETQLISTSTGSSDETGFSSLFTSSTNNKIVMMVRTSKKYDRERTIF
                     EIAEKIREDLATLPEVITYDVSTARFGGAMGGNNVEVEIFGHDFDATNAVAAELKQRM
                     EKLSGARNVDIDREEDRPELKIELDKEKISRHGINTVMMASYVRNRVNGMSAGNFKEQ
                     GDEYPILVRLTEEHRNSITSIEELNIATMQGKLLKLKELADIKEYWGPPNIERKRRER
                     IVTVSATPEGVSLGELAEQIKAEV"
BASE COUNT          588 a          249 c          372 g          561 t
ORIGIN      
        1 aatatttgta agagatgtag ctcaagttag agatactatt aaagatgtta ccttagaaga
       61 aaagattaac ggaaaagatg gagcccgatt gattattatg aaacaatctg gtgccaatac
      121 ggtagaaatt gctaaaaaag tacgcgaaca aattgctcaa gtacaaaaaa cattgcctcc
      181 tgatattcat tttgaaatta tttatgactc ttctgaactt atacaaaatg cagtttttgg
      241 attgtcagaa gcaatcatgt atgcacttat ttttgtggta tttgttgtac ttttcttttt
      301 aggtaaatgg agagcaacat ttattatatc acttactatt cctatatcat taatcgtttc
      361 atttatttat ttgtattaca caggaagttc gttaaatatt atctcacttt cgtctttatc
      421 catagctatt ggtatggtgg tagatgatgc aattgtggtg ctcgaaaata taaacataca
      481 tattgaaaga ggttctagcc ctcgtgaggc ggctatttac gctaccaacg aggtgtgggt
      541 ttctgttatc gctactacct tggtaattgt agccgtattt ttaccgttaa ccttggtagg
      601 aggtattact ggtgtgatgt ttaaagaatt gggttggatt gtaaccattg ttgtatgtgt
      661 ttcaactttg gtagctatta cgctaactcc tagtttgtct gctatgatgt taactatcaa
      721 aaagaaaact gaaaatagta aaacaaatcg ttttagttat caaaatacag tagtaaaatg
      781 gcttgataaa gtagatgagc tatatgcaaa tattttacgc tggtgtttac gaaataaggc
      841 gataacgcta acgcttgcag tgcttatatt tgtaggctct ttagttccag tctttatggg
      901 ggctataggt actgatttta tggcacaaaa tgatgtagga aggcttacca taacaattga
      961 attacaacga ggtacacgct tggaggaagc aagtaaaaca gcacgtataa tagaaagcag
     1021 gattgccaag attgctccag aaacacaatt aatatcgacg tcaacaggtt cgtctgatga
     1081 aacaggtttt tcttctctat ttacttcttc taccaataat aaaattgtta tgatggttag
     1141 gacatctaaa aaatatgata gagaaagaac gatatttgaa attgctgaaa aaattcgaga
     1201 agacttagct accttacccg aagtaataac atacgatgta tctactgcaa gatttggcgg
     1261 tgctatgggt ggtaataatg ttgaagtaga gatttttggt catgattttg atgcaactaa
     1321 tgcagtggcg gcagaattga aacaacgaat ggaaaaatta tcaggtgctc gtaatgttga
     1381 tattgataga gaagaagata gaccagaatt aaaaatagag cttgataaag aaaaaatatc
     1441 tcgccacggc attaatacag tgatgatggc atcttatgtt cgcaatagag taaatggtat
     1501 gtcagccggt aattttaaag agcagggaga tgagtatcct attttggtta gattgacaga
     1561 ggagcatcgt aatagtatta cttctatcga agagttaaat attgctacga tgcaaggtaa
     1621 attattgaaa ttaaaagagt tggcagatat taaagaatat tggggaccac caaatattga
     1681 acgtaaacgt cgtgaacgta ttgtaaccgt atctgctacg ccagagggtg tgtcgctcgg
     1741 tgaattagct gaacaaatta aggcagaggt
//
LOCUS       DAOPUR010000334         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_74380_length_1765_cov_3.990058, whole genome shotgun sequence.
ACCESSION   DAOPUR010000334 DAOPUR010000000
VERSION     DAOPUR010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74380_length_1765_cov_3.990058"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKN90_06085"
     CDS             <1..343
                     /locus_tag="PKN90_06085"
                     /inference="COORDINATES: protein motif:HMM:NF027514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ85877.1"
                     /translation="HEGYASISPRAVQVALQPGMLITNEPGLYKENEYGMRIENVMLV
                     KEVSNGRSGRFLCFETLTLCPIDMQIIDKTMLTEEELIWLEQYQQKVFDELSPYLTEE
                     ERVWLKNKITN"
     gene            387..>1765
                     /locus_tag="PKN90_06090"
     CDS             387..>1765
                     /locus_tag="PKN90_06090"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HNZ85878.1"
                     /translation="MKKYCLIVCIILLPLLVVAQKKEIKAYSIELNLPHLKSEKVYLG
                     RYVMGKPYSNDSIVLDTKGRGVFQGSKPLDEGLFMLYFTDGKYVDFMVGNEQHISVKG
                     DTLHLPANLQFSGASQTKDFFQYMRFLTTLQQDYGRKQEQLKATVDDDTKKQVEKELE
                     ALSQQLLTQQNELIANYPTGMVSVFIKGLRIPDMPEDVENQATSDSLKQVMRYLFYKK
                     HYFDNVNFSDIRTYNTPYIVSNLETYLNRVLVQHPDSIVPAAIDLVEKSKGNDKTFQM
                     MTSFMLNYGVKSTMMGMDKLMVELGNRYYLTGKATWADSTLIESLRKEIKKIETSLVG
                     MKASNMYLANEKGEYSDFYSMFGEQLTVLYFFEPDCGHCKKVTPLLRSFYEKYKNDKR
                     INIVAVYMLLDKKEWSDFIEKNQLQAFTNVWDPNRVSRFWELYDASTTPMMYVMDKNK
                     KILAKKIDV"
BASE COUNT          612 a          220 c          377 g          556 t
ORIGIN      
        1 gcacgaaggt tatgcttcaa ttagtcctcg tgcggtacaa gtggcattgc agcctggtat
       61 gttaattacg aatgaacctg ggttgtataa agaaaacgag tatgggatgc gtattgaaaa
      121 tgtaatgtta gtcaaggagg tgagtaatgg taggagtggt agatttttgt gttttgagac
      181 gctaacgttg tgtcctattg atatgcagat tatagataaa acgatgttga cagaggagga
      241 acttatctgg cttgaacaat atcaacaaaa agtatttgat gaattatcgc cctatttgac
      301 agaagaagaa cgagtatggt taaaaaataa aataacaaat taatgagata cataatttta
      361 tagaaagaaa ttaaattaaa agcatcatga aaaaatattg tttgatagta tgtattattc
      421 tactcccatt gttagttgta gcacaaaaga aagagattaa agcttattct attgaactca
      481 atttgcctca cctaaaaagt gagaaagtgt atttgggaag atatgttatg ggcaagccat
      541 attccaacga ttctattgtg ttggacacaa aagggcgtgg cgtttttcaa ggatcaaagc
      601 ccttagatga gggattgttt atgttgtatt ttacggatgg caaatatgtt gattttatgg
      661 ttgggaatga acagcatatt tcggtgaaag gagatacgtt acatttgcca gcaaatcttc
      721 agtttagtgg agcaagccaa acaaaagatt tttttcagta tatgcgtttt ttaaccacat
      781 tgcaacagga ttatggtcgt aaacaagaac aactaaaagc gacggtggat gacgatacta
      841 aaaaacaagt agaaaaagag ttggaggctt taagtcaaca gttacttaca cagcaaaatg
      901 agctcattgc taactatccc actggtatgg tctctgtttt tattaaaggt ttacgtatac
      961 cagatatgcc agaagatgtt gaaaatcagg caacaagtga tagtctgaaa caggtgatgc
     1021 gttatttatt ttacaaaaaa cattactttg acaatgttaa tttttctgat attcgtacgt
     1081 ataatactcc gtatattgtt agtaatttag aaacctatct aaatagggta cttgtgcaac
     1141 atccagactc cattgttcct gcagcgattg atttagttga aaaaagtaaa gggaatgata
     1201 aaacgtttca gatgatgaca agttttatgc ttaattatgg tgttaagagt actatgatgg
     1261 gaatggacaa gcttatggta gaattgggca ataggtatta tttaacagga aaggcgactt
     1321 gggcagattc tactttaatt gaaagtttac gaaaagaaat taaaaagata gaaacgtcct
     1381 tggtcggtat gaaggcttct aatatgtatt tagctaatga aaaaggggag tactctgatt
     1441 tttattccat gttcggagaa cagctgacgg tattgtattt ttttgagcca gattgtggtc
     1501 attgtaagaa ggttacacca ttattacgta gtttttatga gaaatataaa aatgataaaa
     1561 gaatcaatat tgtagctgta tatatgcttt tagataagaa agaatggtct gattttattg
     1621 aaaaaaatca attacaagca tttacgaatg tttgggatcc aaaccgagtt tctcggtttt
     1681 gggagcttta tgatgcttct actacaccca tgatgtatgt gatggataag aataaaaaga
     1741 ttttagcaaa aaaaatagat gttga
//
LOCUS       DAOPUR010000335         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_74521_length_1763_cov_3.202576, whole genome shotgun sequence.
ACCESSION   DAOPUR010000335 DAOPUR010000000
VERSION     DAOPUR010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74521_length_1763_cov_3.202576"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKN90_06095"
     CDS             <1..534
                     /locus_tag="PKN90_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ85879.1"
                     /translation="DGIVVIDEAYVDFSPEQSWLVEINTYQNIIVLQTFSKAWGLASV
                     RCGMAFASEKIISFFNKVKYPYNINILTQQFVEEQLENTDRKEEWVTMLLGQRTYLVE
                     QLNKLPLIQTIYQTDANFVLVKVEDANAIYRYLVDLGIIVRNRNTVSLCLGCLRITIG
                     TQQENDTLLQALKAYTY"
     gene            546..1682
                     /gene="hisB"
                     /locus_tag="PKN90_06100"
     CDS             546..1682
                     /gene="hisB"
                     /locus_tag="PKN90_06100"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HNZ85880.1"
                     /translation="MDNLKKALFIDRDGTIIAEPPVTFQVDNLQQLEFLPSVIRNLYL
                     IRKHTDFEFVMVTNQDGLGTEVYPQENFDSVQAKMLQVLQNEGVVFDDILIDTSFPEQ
                     NLPTRKPQTGLLTSYIAGGYDLQHSFVIGDRITDVELAKNIGCKAIFLQNNENATTLL
                     KDNNLNDYCALISTDWDEIAAYLVAKERTATVQRTTKETDILVYVNLDGKGKTSIHTG
                     LGFFDHMLDQIGKHSGMDLTIHVKGDLQVDEHHTIEDTALALGEALLVALGNKRGVER
                     YGYCLPMDECLCQVALDFGGRPWLVWDATFTREKVGDMPTEMFMHFFKSLSDTAKMNL
                     NIKAEGANEHHKIEGIFKALARAIKMAIKRDVFNGELPSTKGVL"
BASE COUNT          595 a          271 c          338 g          559 t
ORIGIN      
        1 gatggaatcg tggtaattga tgaagcttat gttgattttt ctccagaaca gtcatggcta
       61 gttgagataa atacgtatca aaatatcatt gtgttacaaa cattctcaaa agcgtggggc
      121 ttggcttctg tgcggtgtgg aatggctttt gcttctgaaa aaattatctc ttttttcaat
      181 aaagttaagt atccctacaa tatcaatata ttaactcaac aatttgttga agagcaactt
      241 gaaaatacag accgaaaaga agaatgggta actatgctgt taggacaacg tacttatcta
      301 gtggaacaat taaacaaact tcctctcata caaacaatat accaaaccga tgccaatttt
      361 gtgttggtaa aagtagaaga tgctaatgcc atttatcgtt atttagttga tttaggtatc
      421 attgttcgaa atagaaacac ggtttcattg tgtttaggat gtttgcgaat aacaattgga
      481 acacaacaag aaaatgacac actattacaa gctttaaaag catatacata ctaattctat
      541 cacgaatgga taatctgaaa aaagcccttt ttatcgatag agatggtact atcattgcag
      601 agcctcctgt tacatttcaa gtagataatc tacaacaatt ggagtttttg ccatcagtaa
      661 ttcgtaattt gtatcttata cgcaaacata ctgattttga gtttgttatg gtaaccaatc
      721 aggatgggct tggaacagag gtttatcctc aagaaaattt tgattcggta caagcaaaaa
      781 tgttgcaagt attacaaaat gagggagttg tattcgacga tattttgatt gatacttctt
      841 ttcctgagca aaatttgcct acacgaaaac cccaaactgg attgttaaca tcctacattg
      901 ctggaggtta cgatttacaa cattcttttg taattggaga tagaattacg gatgtagaat
      961 tagctaaaaa tataggatgt aaagcgattt ttttgcaaaa taatgaaaat gcaacaaccc
     1021 tgttaaaaga taacaacctg aatgattact gtgctttaat atcaacggat tgggacgaaa
     1081 ttgctgcata tttagttgca aaagaacgca ccgctactgt gcaacgaacc accaaagaaa
     1141 cagatatttt agtgtatgtt aatttagatg gcaaaggtaa aacttctatt catactggtt
     1201 taggtttttt cgatcatatg ttagaccaaa ttggtaaaca ttcgggaatg gatcttacca
     1261 tacacgtaaa aggagatttg caagttgatg agcatcatac catagaagat actgctttag
     1321 ctttgggaga agccctttta gtagctttag gaaacaaaag aggtgtagag cgttatggct
     1381 attgtttgcc catggacgaa tgcttatgtc aagttgcttt ggattttggt ggacgaccct
     1441 ggttggtatg ggatgctact tttacaagag agaaagtagg agatatgcca acagaaatgt
     1501 ttatgcactt ttttaaatca ttgagcgata ctgctaaaat gaatttgaat ataaaagctg
     1561 aaggagccaa cgaacatcat aaaattgaag gaatatttaa agcccttgct cgtgctatta
     1621 aaatggctat taaaagggat gtttttaatg gagaattgcc tagtacgaaa ggtgttttat
     1681 aattattttt tataatactt caaaaatgaa tacattacgt ttcctcgcta tcatcccagc
     1741 acgttatgca tcaacacgtt ttc
//
LOCUS       DAOPUR010000336         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_75124_length_1754_cov_2.952913, whole genome shotgun sequence.
ACCESSION   DAOPUR010000336 DAOPUR010000000
VERSION     DAOPUR010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75124_length_1754_cov_2.952913"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..875)
                     /locus_tag="PKN90_06105"
     CDS             complement(231..875)
                     /locus_tag="PKN90_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ85881.1"
                     /translation="MLIEQVPQCIRRFFQPVIWRGNAKEKKIFLTFDDGPTPEITPWI
                     LDLLDMYNVKATFFCIGNNVEKYPDLFLEIQQRGHQVGNHAYTHKRGMFTDTKIFFED
                     IARAQKLINSDLFRPPHGHITPRQIKKLKHTFKIVLWDVITRDYDATLPPEIILQNVK
                     KYSRNGSIIVFHDSLKAEKNLRYALPKSLAFWKEKGYECSLIHPTTTTSSDKNR"
     gene            complement(884..>1754)
                     /locus_tag="PKN90_06110"
     CDS             complement(884..>1754)
                     /locus_tag="PKN90_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85882.1"
                     /translation="ASDDPRTKQDPRTGDTRDFIPATTLSLAVNADNLVKQGAISADE
                     KGSVLPAIEFKLQDRQLLTKSELMILEMLAQNNWERPIYYAVTVGSEMHLGLRKYFQL
                     EGLAYRIVPRTDSIPTGTVHTDMMYDNMMNKFKFGNIADPSVYLDENILRMSKVQRQM
                     FSSLVGALLEEGDSIRALKALDYCMKVVPGTTVPHDFSSTSLAEYYYMLGEKEKATDL
                     INTIGNRSIAYLEWISKLSPQKKASAAYDAHIQLSSLRNIAYITQNYDEKTFEKYYAS
                     LMQYASIFDHLLR"
BASE COUNT          527 a          293 c          325 g          609 t
ORIGIN      
        1 ttgagtatgc gagtgggact tcgaagagaa tggatgcgag tgtgtaataa tggtattatt
       61 aacaatatgc gtatgcgaaa gcaaggtaga acttacccag taagcaataa ataggaatgc
      121 taggaatccg tgtatatagg ttgagtgctt gcgtttcata tttttatggt gcaaaaatac
      181 aacggaagaa tgggagtatt gtaccataaa aaaactacaa gtagtgatat ttatctattt
      241 ttatcactac ttgtagtggt tgtaggatga atgagagagc attcataccc tttttctttc
      301 caaaatgcta atgattttgg tagtgcatag cgtaagtttt tttcagcctt caatgagtcg
      361 tgaaatacaa ttatagaacc gtttctagaa tattttttta cattttgcaa tattatttct
      421 ggaggcaacg tagcatcgta atcgcgtgta ataacatccc aaagaacaat tttaaatgtg
      481 tgcttgagtt tttttatctg tcgaggggtt atatgtccat gtggtggtct aaataaatca
      541 ctattgataa gtttttgagc acgtgcaata tcttcaaaga atatttttgt gtcggtaaac
      601 atccctcgtt tgtgggtgta tgcatgattg cctacttgat gccctcgttg ttgaatttct
      661 agaaacaagt cgggatattt ttcaacatta ttgccaatac aaaaaaatgt agcttttaca
      721 ttatacatat caagcaaatc taaaatccaa ggtgttattt ccggagtagg tccatcatca
      781 aatgtaagaa aaatcttttt ttctttggcg tttcctctcc atattactgg ttggaagaaa
      841 cgccttatac attgaggaac ttgttctatt agcataatga ctattacctc aataaatgat
      901 caaatatact tgcatattgc attaagcttg catagtattt ttcaaacgtt ttttcgtcgt
      961 aattttgtgt gatataagca atatttctga gcgaagatag ttgaatatga gcatcatatg
     1021 ctgcagaagc ttttttctgt ggactcaatt tacttatcca ctctaaatag gcaatggatc
     1081 gattaccaat agtattaatt aaatcagttg ctttctcttt ttctccaagc atataataat
     1141 attcagctaa tgaggtagaa gaaaagtcgt gaggcactgt agtacctgga actactttca
     1201 tacaatagtc caatgctttt aaagccctaa tagaatctcc ttcttctagt aaagctccta
     1261 ctaatgaaga aaacatttga cgttgtactt ttgacatacg cagaatattt tcgtctaaat
     1321 atacagaagg gtcggcaata ttgccaaatt taaacttatt catcatatta tcatacatca
     1381 tgtctgtatg aactgtaccc gttggtatag agtcggtacg aggtactata cgataagcca
     1441 aaccttctaa ctggaaatat ttacgcagac caagatgcat ttctgaccca actgttacag
     1501 cataataaat tggtcgttcc caattgtttt gtgctagcat ttctagaatc attaattcac
     1561 tttttgttag taattgtcta tcttgcaatt taaattcaat agcaggcaat acgctccctt
     1621 tctcgtctgc actaatggct ccttgtttca ctaaattatc tgcatttaca gctaatgata
     1681 gagttgtagc agggataaag tcgcgtgtgt cccctgttct tgggtcttgc ttggtgcgag
     1741 ggtcgtcgct agct
//
LOCUS       DAOPUR010000337         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_75441_length_1749_cov_2.861865, whole genome shotgun sequence.
ACCESSION   DAOPUR010000337 DAOPUR010000000
VERSION     DAOPUR010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75441_length_1749_cov_2.861865"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..>1749
                     /locus_tag="PKN90_06115"
     CDS             42..>1749
                     /locus_tag="PKN90_06115"
                     /inference="COORDINATES: protein motif:HMM:NF037155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNZ85883.1"
                     /translation="MIEDANSDNIIAPFSQIIDPNKNYNDVGSYWDYYEDDYYYDTED
                     YNWEESDDPSKSSYYMYNRHKIVKNVLSSDFGIIAKAGSNNDYFVVVTDLRTTDPIKG
                     VEIEFRNLQNQTMGVTRTNGDGMCKLNLQDKPFVLIAKKGKQRGYLRLSDASSLSLSM
                     FDVSGEEIKKGVKGFLYGERDVWRPGDIMYLTFILEDKLKSLPANHPVVLELTNPLGQ
                     LTQRIVKTNHVNGFYNFTIRTEPSAPTGNWTAKVSVGNSTFSRAIRIETVKPNRLKIN
                     LTFPSDLLKMGQNHQGSLEVNWLYGSPASNARVEIDAIARAAQTSFKECVGYVFDDPT
                     KSFVSEDIALYKGAVNAQGKASIPCKFNFSNEAPGMVSIEMKTRAFEVGGDFSIDRSL
                     FKYSPYKKYVGVKMPEGKGWNNALYSDEKNLIPIALVDENGKAANGKVKIEIFNIYWR
                     WWWDQSEEDNLADYVSNENRNLIKTDYIDIRNGRGVYEMNLNEEYWGRKLIKITDMSG
                     NHSTGAIFYTTYKGWWSNASSENPGGAEILMFKTNKKQYKVGEIVSIELPFSHKGRAL
                     ISI"
BASE COUNT          628 a          268 c          351 g          502 t
ORIGIN      
        1 gtgtagaaat atcttttcga aaaaaacatt ctctttatcc aatgattgaa gatgcaaatt
       61 ccgacaatat tattgcacca ttttcgcaaa tcatagaccc aaataaaaac tacaacgacg
      121 ttggtagtta ttgggattac tacgaagatg actattacta tgatacagaa gattacaatt
      181 gggaagaaag tgatgaccca agtaaatcat cttattacat gtataataga cataaaattg
      241 ttaagaatgt attgtcttca gattttggca ttattgcgaa agctggatca aataatgatt
      301 attttgtagt agttaccgat ttacgtacaa ccgatccaat taaaggtgta gaaattgaat
      361 ttagaaattt acaaaaccaa acgatgggag ttacacgtac caatggtgac ggaatgtgta
      421 aattaaatct acaagataaa ccatttgtat tgatagctaa aaaagggaag caacgtgggt
      481 atttacgttt aagcgatgcg tcgtcattat cgttaagtat gtttgatgtg agtggcgaag
      541 aaataaaaaa aggcgttaaa gggtttttat atggagagcg agatgtatgg cgtcctggcg
      601 atataatgta tttaaccttt atcttggaag ataaactaaa atcattacca gcaaaccatc
      661 ctgttgtgtt agaactaacc aatccacttg ggcaactcac acaacgcata gttaaaacta
      721 atcatgtaaa tggcttttat aattttacta ttcgcaccga accatcagcc cctactggta
      781 attggacagc caaggtcagc gttggtaatt caacattttc tcgcgccatt cgtattgaaa
      841 ctgttaaacc taatcgattg aaaatcaacc tcacttttcc ttctgattta cttaaaatgg
      901 gacaaaacca tcaaggttct ttggaagtaa attggcttta tggttcgccc gcatccaatg
      961 cacgggtaga aattgatgcc atagcaagag ctgctcaaac tagctttaaa gaatgcgtag
     1021 gatatgtatt cgacgatcct actaaaagtt ttgtatcaga agatatagca ttatataaag
     1081 gtgccgtaaa tgctcaaggg aaagccagca taccctgtaa atttaacttt tcaaacgagg
     1141 ctccaggcat ggtgtcaata gaaatgaaaa caagggcatt tgaggtaggt ggcgatttta
     1201 gtatagatcg ttctcttttt aaatactctc cgtataaaaa atacgtaggt gttaaaatgc
     1261 ccgaaggtaa aggatggaat aatgcactct actctgatga aaagaatctt attccgatag
     1321 ccttagtaga tgaaaatgga aaagcagcaa atggaaaagt aaaaatagaa atctttaaca
     1381 tctattggcg ttggtggtgg gatcaatcag aagaagataa tttggctgat tatgtatcga
     1441 atgagaatag aaatctaatt aaaacagatt atatcgacat tagaaatgga cgtggtgtgt
     1501 atgaaatgaa cctaaatgaa gaatattggg gtagaaaact cattaaaatt accgacatga
     1561 gtggaaatca tagtacgggt gcaattttct ataccactta taaaggatgg tggagtaatg
     1621 ctagtagcga gaatccagga ggggcagaaa tcttgatgtt taaaacaaat aaaaaacaat
     1681 acaaggttgg agaaattgta tccatcgaat tgccttttag tcacaaagga cgtgcattaa
     1741 taagcatag
//
LOCUS       DAOPUR010000338         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_75864_length_1742_cov_2.848251, whole genome shotgun sequence.
ACCESSION   DAOPUR010000338 DAOPUR010000000
VERSION     DAOPUR010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75864_length_1742_cov_2.848251"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..>1742)
                     /locus_tag="PKN90_06120"
     CDS             complement(245..>1742)
                     /locus_tag="PKN90_06120"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNZ85884.1"
                     /translation="GGGEKRIENQHKKGKYTARERIAMLLDEASFEEIDMFIKHRCTN
                     FGMDKKEAILGDGVVTGCGTIDGRLVYVFAQDFTVEGGSLSETMALKICKVMDLSLKM
                     GAPIIGINDSGGARIQEGVTALAGYAEIFQRNILTSGVVPQISAIFGPCAGGAVYSPA
                     LTDFTIMTKGTSYMFLTGPKVVKQVLGEDVTQEELGGASIHSTKSGVAHFAVDSEEEG
                     ILCIRKLISFLPQNNLEEPPLVSCSDPIDRLEDSLNDIIPDSPNKPYDMYEVLGSVID
                     NGEFLEVHKDYAPNIIVGFARFNGTSVGIVANQPKVLAGVLDSNSSRKAARFVRFCDA
                     FNIPIVTFVDVPGFLPGTQQEYNGVILHGAKLLYAFGEATVPKITITLRKSYGGAYCV
                     MSSKHLRGDVNYAWPTAEIAVMGPEGAIEVLYGKEIAASDNPTELQKEKVEEYKELFA
                     NPYNAARYGYIDDIIEPRNTRFRIIRSLQQLATKKLTNPLKKHDNLPL"
BASE COUNT          555 a          379 c          268 g          540 t
ORIGIN      
        1 gccggctcaa gggtatcatt tgtcaaataa caccctccaa tatcaataac acgatggctg
       61 ggattatata tttctatcca accacttcta tttccataat catccaccaa gttctcagta
      121 ttcgttacta gtagttcatt tataaccaag tttaaacttt ttgcagacat gttaacagag
      181 aacaaactaa gaaggagtaa gagcgtaata gataaacgca tttttatata gttatgtatc
      241 ataattataa cggcagatta tcatgctttt taagcggatt agttaatttt tttgttgcca
      301 attgttgcaa tgagcgaata attcgaaaac gggtattgcg tggttctata atatcatcaa
      361 tataaccata tcgtgcagca ttatagggat tagcaaacaa ctctttgtat tcttctactt
      421 tttctttttg tagttcagtt ggattatcag aagcagctat ttcttttccg tacaatacct
      481 cgatagctcc ttcaggtccc atcacagcaa tttctgctgt aggccacgca taatttacat
      541 ctcctcgcaa gtgtttagaa ctcatcacgc aataagctcc accatacgac ttacgtaggg
      601 ttattgttat ttttggcaca gtagcttcgc caaatgcata aagcaattta gcaccgtgca
      661 aaataacacc attgtattct tgttgtgtac caggtaaaaa tccaggaaca tctacaaatg
      721 ttacaatagg aatattgaag gcatcacaaa aacgcacaaa acgtgctgct ttacgagatg
      781 aattactatc caaaacacca gctaacacct taggttgatt agcaacaata cctaccgaag
      841 ttccattaaa acgagcaaaa ccaacgataa tattaggggc ataatctttg tgtacttcta
      901 aaaattcgcc attatcaatt acagaaccta atacctcgta catatcataa ggcttattgg
      961 gagagtcagg tataatatca ttaagcgaat cttccaatct gtcaattggg tcagagcatg
     1021 acaccaaagg aggttcttct aaattatttt gaggcaaaaa gctaattaat ttgcgaatac
     1081 acaaaatacc ctcttcctct gagtcaacag caaaatgagc tactcccgac tttgtagaat
     1141 gaatacttgc accacctagt tcttcttgtg taacatcttc tcctaacact tgtttcacaa
     1201 ctttaggtcc agttaaaaac atataactag tacctttggt cataatagta aaatcagtta
     1261 aggctggaga ataaacagca ccaccagcac atggaccaaa aatagcagat atttgaggca
     1321 ctacacctga tgttaaaata ttgcgttgga aaatttctgc ataaccagct aatgcagtta
     1381 ctccctcttg aattcttgct ccaccagagt catttatgcc tatgataggt gctcccattt
     1441 tcaaagacaa gtccataact ttgcaaatct tcaatgccat tgtttcagac aatgatcctc
     1501 cttcaactgt aaaatcttgg gcaaagacgt ataccaatcg cccatcaatg gtaccacatc
     1561 ctgttaccac cccatctcca agaatggctt cttttttgtc cataccaaaa ttggtacaac
     1621 gatgcttaat aaacatatct atttcctcaa aactagcttc atcaagtaac atagctatac
     1681 gctctctagc tgtatacttg cctttcttat gttggttttc gatacgtttt tctccgcctc
     1741 ct
//
LOCUS       DAOPUR010000339         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_76302_length_1735_cov_3.368452, whole genome shotgun sequence.
ACCESSION   DAOPUR010000339 DAOPUR010000000
VERSION     DAOPUR010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76302_length_1735_cov_3.368452"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..979
                     /locus_tag="PKN90_06125"
     CDS             134..979
                     /locus_tag="PKN90_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85885.1"
                     /translation="MKRLLIALILTCIPAHYVFSYTLTGWFEESKISIFGGVATHHIE
                     KRNSSEDVNLMNYLSYRNEFYLGVDAHVYLHEQWQFNIKTYLNSSLRTDDVFAKLIYM
                     PHEKIGVTLGYASIPVQYGLYEIHEQPQYYTTIPNSDDIYDFAKNELFLAGLAFPFEG
                     GKFSGTIYLQGGVSYLPNDFSFSWQKKYDSNERRLVEYDIPESYAFIIQPEVEIRWEI
                     AKPTTDTSIGLQFQGKWTYSKRNIDYTRNIYTWTTDNKQTEIISHPKQSIEGVDWNVG
                     LFYIF"
     gene            complement(986..1534)
                     /locus_tag="PKN90_06130"
     CDS             complement(986..1534)
                     /locus_tag="PKN90_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ85886.1"
                     /translation="MNSFYNLVSSRQSDRAFLPKGIEDEKLQRILASAQLAPSACNAQ
                     PWHFVVVNETDKRLQIADAMASKVLAMNHFTKQAPVQIVVVEEKANISSSFGSWAKSK
                     HFPHIDIGIVASHICLAAADEGLGTCMIGWFDEKKIKNILSIPDEKRVLLVIVMGYSA
                     QETRPKKRKKLNEIVSYNSYKK"
BASE COUNT          563 a          329 c          304 g          539 t
ORIGIN      
        1 gaatttcatc ttatgtgcaa ggaaacactt tctctcaaca acgctcaatt tagaataatc
       61 gttgaattat cgaatgggca agagttgagt attgatacag cattacttga tttagttact
      121 aacgattaag cacatgaaac gactactcat tgcacttatt cttacttgca tcccagcaca
      181 ttacgtattc agctatacac tgactggatg gtttgaagaa tcaaaaattt ccatttttgg
      241 aggagttgcc acgcatcata tagaaaaaag aaatagctct gaagatgtaa atctgatgaa
      301 ctacctatcg tatagaaatg agttttatct aggcgttgat gcacatgtgt acttacacga
      361 acaatggcaa tttaacatca aaacatacct gaacagctcc ttgcgaacag acgatgtgtt
      421 tgccaaacta atctatatgc cacatgaaaa aattggggtt actttgggct atgcatctat
      481 ccctgttcaa tatgggttat atgagataca cgaacagcca cagtattata ctacaattcc
      541 aaactcagat gatatatacg attttgcaaa aaacgaactg tttttagcgg gtcttgcatt
      601 tccttttgaa ggagggaaat tctcaggaac tatttactta caaggaggag ttagttatct
      661 gccaaacgat ttttcgttct cttggcaaaa gaaatacgat agcaacgaac gtagattagt
      721 agaatatgat atccccgaaa gttatgcatt tatcattcaa cccgaagtag aaatacgttg
      781 ggaaatagca aaacctacaa cagataccag cataggtctt caatttcaag ggaaatggac
      841 ttattcgaaa cgaaatatcg attatacacg taacatatac acttggacta cagacaacaa
      901 gcaaacggaa ataatttcgc atccgaagca atcaatagaa ggggtagatt ggaacgttgg
      961 cttgttttat attttctaaa aaaccctatt ttttgtagct attgtacgac actatttcgt
     1021 tcaatttttt gcgttttttg ggtcgagttt cttgagctga ataacccatt acgattacta
     1081 gcaataccct tttttcatca ggtatagaaa ggatgttctt aatcttcttt tcatcaaacc
     1141 acccaatcat gcaggtaccc aagccttcgt ctgctgcagc taaacaaata tgggaagcta
     1201 ctatcccaat atcaatatgt ggaaaatgtt tactttttgc ccaactgcca aaactagaac
     1261 tgatgtttgc tttttcttct actactacaa tctgaacagg ggcttgtttg gtaaaatgat
     1321 tcattgccaa cactttagac gccattgcat cagctatttg caatcgctta tctgtttcat
     1381 ttaccaccac aaaatgccaa ggttgtgcat tgcatgccga aggagccaac tgagcagagg
     1441 cgagtatccg ctgtaatttt tcatcttcaa taccttttgg caaaaaggct ctatcgcttt
     1501 gtcgggaaga gacaagatta taaaaggaat tcataagcat tactattagt attgtacaaa
     1561 aatagaaatt tttctggtaa aattcgttta aaacttccaa ttcactaaaa tgcggatatc
     1621 tgttttttga tttccataaa tagtttctcc ttcagagcct atctcatttc tgtcggcata
     1681 ccaagtatgt gcaaattttg cataccaagt aagttgaggg aaaggcttat aaaca
//
LOCUS       DAOPUR010000340         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_76441_length_1733_cov_3.399881, whole genome shotgun sequence.
ACCESSION   DAOPUR010000340 DAOPUR010000000
VERSION     DAOPUR010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76441_length_1733_cov_3.399881"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..821
                     /locus_tag="PKN90_06135"
     CDS             189..821
                     /locus_tag="PKN90_06135"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNZ85887.1"
                     /translation="MEDIVVYVEPSNNLANTLLLVEKTTPYNINQINWENFPYKPTCK
                     VHIGWNEDYIYLLFKVEEKHTLAQETVLHGNVSQDSCVEFFVSFDNRNTYYNFEFNAI
                     GSMHVCCRNCDGTNKIAFTDKQLAAIETLTSFEKFTPLNVYNTHWQLGIAIPATLFNK
                     SDWYKNTIWANFYKCGDKLQEPHYLSWKSIVSPKPQFHVPHFFGALRCVK"
     gene            complement(835..>1733)
                     /locus_tag="PKN90_06140"
     CDS             complement(835..>1733)
                     /locus_tag="PKN90_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNZ85888.1"
                     /translation="RSMSFSEVAKKQGFQDGDILFAADGKTLKRLDEDNFRNILNARQ
                     VLVNRAGDLTMVQLTEDFPQQLIASNEGFTSYRIPFVVREVMPETPAFGAGLQKGDSV
                     VSVNDDVLCTQDEFSNVFLANKNTQIMIGFYRQGDFLSTYITPDSAGMIGVYLTPFYE
                     VYPTTKVTYGVLKSIPAGIKKGVKKFTGYASDMKHLMSKQGAESIGGFGSITQLFPPF
                     FSFEIFLEITAYLSVILAFMNILPIPALDGGHIAFLTYEVITRRKVSQEVMMTAQKIG
                     LLLLLFLLFYANGMDVVRAFFR"
BASE COUNT          615 a          355 c          289 g          474 t
ORIGIN      
        1 ttgctcaaaa acgaaaaccg acagaagaaa aaaatcctgc ctttcacgag aaaaaagaaa
       61 agaacaaaaa agtaaattta ggaggttcgt accgcagaga gttggcaaaa aaatacaaaa
      121 agcccaaaac aaagggagat aaaattgcca acagaaaaaa gaaaaaataa ataattgtat
      181 tgatatctat ggaggatata gttgtatatg tagagccaag taacaatctt gcaaatacac
      241 ttttgttagt agaaaaaact accccgtaca acatcaatca aattaattgg gagaatttcc
      301 catacaagcc tacttgcaaa gtacatattg gttggaacga agactatata tacctactgt
      361 ttaaggtaga agaaaaacac accttggcac aagaaaccgt actgcatgga aacgtatcgc
      421 aagatagttg tgtggaattt tttgtatctt tcgacaaccg taacacctac tacaattttg
      481 aattcaatgc cattggctct atgcatgtat gttgtcgcaa ctgcgatggc acaaacaaaa
      541 ttgcatttac tgacaaacaa ttagctgcaa ttgaaaccct gacgagtttt gaaaaattta
      601 cacctctcaa tgtgtataac acccattggc aattaggtat tgccatacct gctacattgt
      661 tcaataagag tgattggtat aaaaatacta tttgggcaaa tttttacaaa tgtggagata
      721 aattgcaaga acctcattac ctatcgtgga aatccatcgt aagcccaaaa ccacaatttc
      781 atgtacccca cttttttgga gctttgcgtt gtgtgaaata agatttaagg cattttacct
      841 aaagaaagcc ctaaccacat ccattccatt ggcataaaac agcaagaata gcaacaacaa
      901 caagccaatt ttttgtgctg tcatcattac ctcttggctc actttacggc gtgttatcac
      961 ttcgtaagtt aagaatgcaa tatgcccacc atctaaagct ggtataggta atatattcat
     1021 aaacgccaat ataacagaca gatatgccgt tatttccaaa aatatctcaa acgaaaaaaa
     1081 cggagggaac aactgagtaa tggaaccaaa tccacctata ctttcagcac cttgcttgct
     1141 cattagatgc ttcatatcgc tagcatatcc tgtaaatttc ttcactcctt ttttaattcc
     1201 cgcagggata gacttcagca caccatacgt aaccttggta gttggataca cctcataaaa
     1261 tggagtaaga tacaccccaa tcatgccagc agaatcaggt gtaatatacg tagagagaaa
     1321 atcaccttga cgatagaacc caatcataat ttgggtattt ttattggcta gaaatacgtt
     1381 tgaaaactca tcttgtgtac aaagcacatc atcatttact gacaccacac tatccccctt
     1441 ttgcaaacca gcaccaaaag cgggtgtttc gggcattact tctctcacca caaaaggtat
     1501 acgatacgaa gtaaatcctt cgttggaagc gataagttgt tgtggaaaat cttcagtcaa
     1561 ttgcaccata gtcaaatcgc cagccctatt taccaatact tgcctggcat tgagtatgtt
     1621 acggaaatta tcttcatcta agcgttttaa tgttttgcca tcagccgcaa ataaaatatc
     1681 gccatcctga aaaccttgtt ttttagcaac ttctgaaaac gacatagatc gga
//
LOCUS       DAOPUR010000341         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_78115_length_1710_cov_2.328097, whole genome shotgun sequence.
ACCESSION   DAOPUR010000341 DAOPUR010000000
VERSION     DAOPUR010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78115_length_1710_cov_2.328097"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PKN90_06145"
     CDS             complement(<1..962)
                     /locus_tag="PKN90_06145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ85889.1"
                     /translation="MKKSKHTILVPITDSSDYFAVNPLHKSADILSENEAKCLKNNID
                     VEGNFTRNKYIVDEDTEKRAFKLAYLDFIDKREDEETQLFFVPNYSCNFACSYCYQEG
                     YNPIQQKLTTEIIDAFFAYVATQFAQKKKYITIFGGEPLLPNEQQRELIAYFIKKATT
                     AQLDIAIVTNGYTLLSYIDILKKARIREIQVTLDGTEQVHNNRRYLKGKQSTFNQIVE
                     GIDACIEAEMPINLRMVVDRENIDNLVEFSQFSIDKVWTKSPYFKTQIGRNYELHFCN
                     STPDKLFDRATLYKKIYELLKEHPHIAEFYKPAFSISKYIAENE"
     gene            complement(1208..>1710)
                     /locus_tag="PKN90_06150"
     CDS             complement(1208..>1710)
                     /locus_tag="PKN90_06150"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNZ85890.1"
                     /translation="VVRLAETVGNNGYVYGIDISEGMIAKAQSLIEKFDITNAKIIQS
                     NLENIPLQDNSVDVVISNCTINHASNKQAVWSEIFRILKPAGRFVVSDIYATHEIAAE
                     YKNDPQAVAECWAGASTRATYLYTLEDTGFENINILEESLPYAKGKAEVASFTVTGKK
                     PIDKIR"
BASE COUNT          496 a          302 c          306 g          606 t
ORIGIN      
        1 tcttcgtttt cagcaatgta ttttgatata gagaaagccg gtttataaaa ttcagcaatg
       61 tgtggatgtt ctttgagcaa ttcatatatt tttttgtaaa gagtagccct atcaaataat
      121 ttgtcaggag tactgttaca aaaatgtaat tcgtagtttc tacctatctg agtcttaaaa
      181 tagggcgatt ttgtccacac tttgtcaata gaaaattgag aaaactctac taaattatct
      241 atgttctctc tatcaactac catacgtaag tttatgggca tttcagcttc aatgcatgca
      301 tctataccct caactatttg attaaaagtg ctttgtttgc cttttaaata ccttctgttg
      361 ttgtgtacct gctcggttcc gtcaagcgta acttgaattt cgcgaatacg tgcttttttt
      421 agtatgtcta tatacgaaag caaggtgtag ccatttgtta caatggcaat atcaagctga
      481 gccgtggtag ctttttttat aaaataggca ataagttcgc gttgttgttc gtttggcaac
      541 aagggttcgc ctccaaaaat cgtgatgtac ttttttttct gtgcaaattg tgtggcaaca
      601 tacgcaaaaa atgcatctat tatctccgtt gttagttttt gctgaatggg attatatcct
      661 tcttgatagc aatacgaaca agcaaaattg cacgaataat tgggcacaaa aaacaattga
      721 gtttcttcat cttcacgttt gtcaataaaa tcgaggtagg ctaatttaaa agctcgtttt
      781 tcagtatctt catctacgat gtacttgttt cttgtaaaat taccttccac atctatgttg
      841 ttttttaagc atttagcttc attttcagag aggatgtcgg ctgatttgtg aagcggattg
      901 actgcaaaat aatcacttga gtcagtaatt ggaacaagta tggtgtgttt ggattttttc
      961 atgcaaaaaa ttgtattaaa ataggggtgt taaaaatcta actatgaata aaatatatcg
     1021 gggaacgaat tccccgatac ggttactctg catagtacac ttagtcgtga caaccagcca
     1081 ctatgctcga aactttagca caacattgag cttgtttcga tgagttgctg ttacatgcac
     1141 agcgtttttt cactaaactt ttcataaaaa aatagtatta aattaagtaa aagtgaaaga
     1201 tccgttgtta tcggatttta tctatcggtt tttttcctgt tacagtaaaa ctggctactt
     1261 cagctttccc tttggcatag ggcaaagatt cttcgagtat attgatgttt tcaaaaccag
     1321 tatcctcaag tgtatataaa taagtagcac gtgtagatgc acctgcccaa cattcagcta
     1381 ctgcttgtgg gtcgttttta tattcagccg caatttcgtg agtggcgtaa atatcactta
     1441 ccacaaatcg accagctggt tttaaaatgc gaaaaatctc agaccacacc gcttgtttat
     1501 tggaggcatg atttatggtg caattagaaa taactacatc tacgctgtta tcttgtagtg
     1561 ggatattttc caaattactc tgaatgattt ttgcatttgt tatgtcgaat ttttcaatca
     1621 acgattgtgc tttggcaatc attccttcgg atatatctat accatataca tacccattgt
     1681 tgcctaccgt ttctgccaaa cgtacaacat
//
LOCUS       DAOPUR010000342         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_80163_length_1682_cov_2.795943, whole genome shotgun sequence.
ACCESSION   DAOPUR010000342 DAOPUR010000000
VERSION     DAOPUR010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80163_length_1682_cov_2.795943"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKN90_06155"
     CDS             <1..633
                     /locus_tag="PKN90_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85891.1"
                     /translation="GNTFDNCYIHGRALAANNYSFSSASRDGITVLSPSFTGYDVLAI
                     ILGNQQTSKTFTRTFTVYPTDLQTLITNYSKQNGKIIVSGAYIASDITRKKDKSEMQF
                     AQNILKYKLRMPQASVNATMVDVFSAKKRQREYSCFTQPNPYQYAVQQVDAIQPTDKN
                     SSVLLRYTENNTAAAIGYKGIYKTIVCGFPFETLTSTEQQNELMRLFVNY"
     gene            656..>1682
                     /locus_tag="PKN90_06160"
     CDS             656..>1682
                     /locus_tag="PKN90_06160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNZ85892.1"
                     /translation="MKISAFIPYENDEQVAQTIASLQGSNLIGKIFLLATKKASTSTK
                     FDAQTLTIDTLTSTATMRLIADNCTNDFALIYLKYTSLYMGAYAIERLAQVANDTQAG
                     MVYTDYYQQTTTGTAKVPVNDYQIGSVRDDFNFGSLLFFATKVLQSSVASCSKDFAHA
                     GLYQLRLTVSEQSQLVHLNEFLYTEIEQDTRKSGEKQFDYVDPKNRAVQIEMEQVCTQ
                     HLKNIGAYLTPIFPAVNLHQELFEYEASVIIPVKNRVKTIEDAIVSVLKQETNFPFNL
                     LIIDNHSTDGTTELIQSYAKKNASIVHIIPQRNDLGIGGCWNEGVHHEKCGKFAVQLD
                     SDDLYETE"
BASE COUNT          599 a          344 c          285 g          454 t
ORIGIN      
        1 gggaatacgt ttgataattg ctacattcat ggaagggctt tagcggcaaa caactattca
       61 ttttcgtcag cttcacgcga tggcattacg gtattgtctc catcatttac tggatacgat
      121 gttctcgcta ttatacttgg taatcaacaa acgagtaaaa cgtttacccg tacttttact
      181 gtatatccta ccgacttgca aacactaatt acgaattaca gcaaacaaaa cggcaaaatt
      241 atcgtaagtg gagcatatat tgcatctgac ataacaagaa aaaaagacaa aagcgaaatg
      301 caatttgcac aaaacatact caaatacaaa ctacgcatgc cacaggcttc cgtaaatgca
      361 acgatggttg acgtgttttc tgcaaaaaaa cgccaacgtg agtacagttg ttttacccaa
      421 ccaaacccct atcaatatgc tgtgcaacaa gtagatgcca tacaaccaac agataaaaat
      481 agttccgttt tactacgtta taccgaaaac aatacagcgg ctgccattgg gtacaaagga
      541 atatacaaaa ccattgtttg tggtttccct ttcgaaacac tcacaagtac tgaacaacaa
      601 aacgaactaa tgcgattatt cgtcaattat taatcatcaa tataacatca agaatatgaa
      661 aatttcagct tttatccctt acgaaaatga tgaacaagta gctcaaacaa ttgcttcgtt
      721 acaaggaagt aatttgatag gtaagatttt tttgttagca acaaaaaaag catcaactag
      781 cacaaaattc gatgcacaaa cactaacaat tgacacccta accagtacgg caacaatgcg
      841 gttaattgct gataattgca ccaatgattt tgcccttatc tacctaaaat atacatcctt
      901 atacatggga gcttatgcta tagaacgatt ggcacaagta gcaaacgaca cgcaggcagg
      961 tatggtatat acagactatt accaacaaac aaccacaggc actgctaaag ttccggttaa
     1021 cgactaccaa attgggagtg ttcgcgatga ttttaatttt ggttcattat tgttttttgc
     1081 gacaaaggta ttgcaatcat ctgttgctag ctgctcaaaa gattttgccc acgctgggct
     1141 gtatcaactc cgattaaccg tatcagaaca atcacaactt gtacacttaa acgaattctt
     1201 atataccgaa atagaacaag atacccgcaa aagtggcgaa aaacaatttg attacgttga
     1261 cccaaaaaac cgagcggtac aaattgaaat ggaacaagtg tgtacacaac accttaaaaa
     1321 tattggagct tacttaacgc cgatttttcc agcagttaat ttgcaccaag aactatttga
     1381 atacgaagcc tctgttatca tccctgtaaa aaatagggtt aaaacaattg aagatgccat
     1441 tgtttctgtg ttaaaacaag aaacgaattt tccattcaat ttgctcatta tcgacaacca
     1501 ttctactgat ggaacaacag aattaataca gtcttatgcc aaaaagaacg cttctattgt
     1561 acatatcata cctcaacgca acgatttggg tataggtggt tgctggaatg aaggtgtaca
     1621 ccacgaaaaa tgtggaaaat ttgccgttca gcttgatagt gacgacctct acgaaacgga
     1681 aa
//
LOCUS       DAOPUR010000343         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_80310_length_1680_cov_2.971692, whole genome shotgun sequence.
ACCESSION   DAOPUR010000343 DAOPUR010000000
VERSION     DAOPUR010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80310_length_1680_cov_2.971692"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKN90_06165"
     CDS             complement(<1..439)
                     /locus_tag="PKN90_06165"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNZ85893.1"
                     /translation="MYSEEDVNWLTENGITPESLEKQLQIFTKGVEPPAIKRIATCND
                     GIRVVNDAEVEMYQTAWNDYIENNPDKTTHFIPASGTANRLFRALYRFYYSEHTEPQT
                     NIEKNFFKHLQSFAFFNELNVICIQKEKKDIHELIEQKQYKLIL"
     gene            629..1567
                     /locus_tag="PKN90_06170"
     CDS             629..1567
                     /locus_tag="PKN90_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018032.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNZ85894.1"
                     /translation="MNIPTHFSPTKRYLDYSQYMLAFFPERIQKISIHAGFTCPNRDG
                     SKGYGGCTFCNNESFSPAYCSSHTSISQQIEEGISFFGKKHAHQKYLAYFQSYTNSYG
                     SMEVIKQKYSEAIHHRAISGIVIGTRPDCMSDELLDYLHEIQKNTFVLVEYGVESTNN
                     NTLELINRGHTYEESVDAIMRTHAKGIPVATHIILGLPSENRETILNHAKQLATLPLA
                     IIKLHQLQIIKNTPLAKQYTDNPSFIPLYSLQEYIDLCIDFMEHIPSHICLERFVSQT
                     PTEYRIAPNWGIKNHEFIAKLDKRLQERNTFQGRLC"
BASE COUNT          547 a          316 c          272 g          545 t
ORIGIN      
        1 cgagaatgag cttatactgc ttttgttcaa ttaactcgtg tatatctttt ttttcttttt
       61 gaatgcaaat cacatttagc tcattgaaaa aggcaaaact ttgcaaatgt ttaaaaaaat
      121 tcttttcaat atttgtttga ggttctgtat gttctgaata ataaaaacgg tacaatgctc
      181 taaaaagacg attagctgtg cctgatgctg gtataaaatg agtggtttta tctgggttat
      241 tttcaatata atcattccat gctgtttgat acatttctac ctcagcatca tttacaacac
      301 gaataccatc gttgcatgtt gctatacgtt taatagctgg cggttcaact cccttggtaa
      361 agatttgtaa ctgtttttct aacgattcgg gcgtaatacc attttctgta agccaattta
      421 catcctcttc actatacatc ctatcttact tttaattaag aacagcaaaa atacaactta
      481 atagtgatac ttcaaaaaat attacgtact gatttttatt cgttctattt caatacttca
      541 attttgtatc gtacctttgc acgtagcttt tttgcatgca tggatattgt attaagccaa
      601 ctatgccgca ctataaaaat aataaagcat gaatattccg acccattttt cgccaacaaa
      661 acgatatctt gattattcac agtatatgct tgcgtttttt ccagaacgaa tccagaaaat
      721 atctattcat gcgggattta catgccccaa tagagatgga agcaaaggat atggaggttg
      781 tacgttttgc aacaatgaat cgtttagtcc agcatattgt agctctcaca catccatttc
      841 acaacaaata gaggaaggta tttctttttt tggcaaaaag catgcacatc aaaaatatct
      901 tgcctacttt caatcgtata caaattcgta cggaagtatg gaagttatta aacaaaaata
      961 cagcgaagcc atacaccatc gagctatttc gggcattgta attggcacac gacccgactg
     1021 catgtcggac gaattgcttg attacctgca tgaaatacaa aaaaatactt ttgtactagt
     1081 tgaatatggt gtagaaagta caaataacaa cacgcttgaa ctgataaatc gaggacatac
     1141 gtacgaagaa agtgttgatg ccatcatgcg aacacacgcc aaaggcattc ctgtggcaac
     1201 acatattatt ttgggattgc catctgaaaa cagagaaacg atattaaatc atgccaaaca
     1261 attagccaca cttccgctgg ctatcatcaa attgcatcaa cttcaaatta taaaaaatac
     1321 acctttagcc aaacaataca cagataatcc atcgtttatt ccgttgtaca gtttacagga
     1381 atatattgat ttgtgtatcg attttatgga acatatacct tcgcatattt gccttgaacg
     1441 ttttgtatcg caaacgccta ccgaatatcg tatagcaccc aattggggca taaaaaacca
     1501 cgagtttatt gctaaactgg ataaacgttt gcaagaacgc aacacatttc aaggtcgttt
     1561 gtgttaatta cgacccttta attgttgaga gtaatgtact cacagctaac ggaatagatg
     1621 gttgattttg taattgcttt ataaattcac tgcctataat tgcaccatta gcatatgtgc
//
LOCUS       DAOPUR010000344         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_80925_length_1672_cov_2.776747, whole genome shotgun sequence.
ACCESSION   DAOPUR010000344 DAOPUR010000000
VERSION     DAOPUR010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80925_length_1672_cov_2.776747"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKN90_06175"
     CDS             complement(<1..809)
                     /locus_tag="PKN90_06175"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNZ85895.1"
                     /translation="MVDLGINIGNLQLKNPVMTASGTFGYGTEFSDFFDISALGGIII
                     KGTTLHHREGNPYPRMAETPAGMLNAVGLQNKGVEYFITSIYPSLKTIDTHVLVNVSG
                     STIEEYVQAAEKINDLDKIPAIELNISCPNVKKGGMGFGTSCASAAEVVKAVRKVYTK
                     SIMVKLSPNVTNIAEIAQAVEAEGADSVSLINTLLGMAVDAENQKPVLSTITGGLSGP
                     CVKPIALRMVWQVAKAVNIPIVGLGGIMNATDAIEFMLVGASAVQIGTANF"
     gene            complement(803..1573)
                     /locus_tag="PKN90_06180"
     CDS             complement(803..1573)
                     /locus_tag="PKN90_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNZ85896.1"
                     /translation="MKKYILDCVVVANIPLNKRYFLLRVSHPHPFPRMLPGQFVELRV
                     DNSSDVLLRRPISIHDMNYETNEMDLLVQIVGAGTLKMSELKKGDLLNMIAPLGNSFS
                     MPTIDMKHVLLVGGGVGVAPLLLLGRVLQQYSCKVSFLLGARTASDLVQLDSFSQYGE
                     VYLTTEDATVGEKGFVTNHPLLKNKVFDKIYTCGPTPMMKAISAYAKTNNIECEVSLE
                     NTMACGFGVCLCCVTDTTEGHKCVCTEGPVFNLTTLKW"
BASE COUNT          560 a          353 c          246 g          513 t
ORIGIN      
        1 ataaaatttg ctgttcctat ctgcacagca gaagcaccta ctaacataaa ttcaatagca
       61 tcagttgcat tcataatacc ccctaagccc acaatcggta tgttaacagc tttagctact
      121 tgccatacca tacgtaaagc tataggttta acacatggac cagacaatcc acccgtaatg
      181 gttgataaaa cgggtttctg attttcagca tcaacagcca tacccagcaa tgtattaatc
      241 aacgatacac tatctgcccc ttcagcttct actgcttgtg caatttcggc tatattggta
      301 acattaggag aaagtttaac cataatgctt ttggtatata ccttgcgtac agcttttact
      361 acctctgcag ctgatgcaca tgatgtacca aaacccatac ctcctttttt tacatttgga
      421 cacgaaatat ttaattcaat agcaggtatc ttatccaaat catttatttt ttctgcagct
      481 tgcacatatt cttctattgt actaccagaa acattaacta acacatgagt atcaatcgtt
      541 ttaagggatg gataaatgga tgtaataaaa tactctactc ctttattttg caagccgaca
      601 gcgtttagca ttcctgctgg agtttctgcc attcgtggat acggattgcc ttcgcgatga
      661 tgcaacgttg ttcctttaat aataattcca cctaacgctg aaatgtcaaa gaaatcagaa
      721 aattctgtac catatccaaa agttccggat gcagtcatta ctggattttt aagctgcaaa
      781 ttaccaatat ttatacctaa atctaccatt ttaatgttgt taaattaaac actggacctt
      841 ctgtacacac acatttatga ccttctgttg tatctgttac acagcataaa catacgccaa
      901 aaccgcaagc catagtattt tctaacgata cttcgcactc aatattattg gtttttgcgt
      961 atgctgaaat ggctttcatc ataggagttg gaccacaagt ataaattttg tcaaaaacct
     1021 tatttttcaa tagcggatga ttagttacaa aacctttttc acctacagtc gcatcttctg
     1081 ttgtaagata aacctcgccg tactgactaa aagaatcaag ttgaactaaa tcacttgcag
     1141 tacgtgcacc aagaagaaat gaaaccttac aagaatactg ttgtaatacc cttcccaata
     1201 ataataatgg ggcaactcct acacctcctc ctaccaaaag cacatgtttc atatcaattg
     1261 ttggcatcga aaatgaatta cccaagggag ctatcatatt aagtaaatct ccttttttta
     1321 actccgacat tttgagggta cctgctccaa caatttgaac taacaaatcc atttcatttg
     1381 tttcgtaatt catatcatga atagaaatag gtcttcttaa taacacatca gaagaattgt
     1441 ctacacgcaa ttcaacaaac tgaccaggta acatacgagg aaaaggatga ggatgtgata
     1501 cacgtaacag aaaatagcgt ttatttaacg ggatatttgc tacaacaaca caatcaagaa
     1561 tgtatttttt cataaaatca ttaggtagaa taatttagta atcaaaacac ccaaaactga
     1621 tatacaaaca acgcttgttt acaaacaaat aatggatggg taaaaacctc ca
//
LOCUS       DAOPUR010000345         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_81064_length_1670_cov_3.214861, whole genome shotgun sequence.
ACCESSION   DAOPUR010000345 DAOPUR010000000
VERSION     DAOPUR010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81064_length_1670_cov_3.214861"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /gene="glyA"
                     /locus_tag="PKN90_06185"
     CDS             complement(<1..378)
                     /gene="glyA"
                     /locus_tag="PKN90_06185"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNZ85897.1"
                     /translation="MKRDTAIFEIIEKEKKRQLKGIELIASENFVSEQVMEAMGSCLT
                     NKYAEGYPGKRYYGGCEVVDLSEQLAIDRLKELFGAEWANVQPHSGAQANAAVFLACM
                     NPRDKFLGLNLSHGGHLSHGSLVN"
     gene            complement(392..>1670)
                     /locus_tag="PKN90_06190"
     CDS             complement(392..>1670)
                     /locus_tag="PKN90_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNZ85898.1"
                     /translation="KIRNLEDDIALSLSALGIRIIAPIPGKGTIGIEVPNKTPQIVSM
                     HSVIASKKFQEAKHELPIVFGKTITNEVFTVDLCKMPHILVAGATGQGKSVGLNAIIT
                     SLLYKKHPSQLKFVLIDPKKVEFSIYSNIEKYFLAKLPDADEAIITDVTKVVQTLNSL
                     TKEMDSRYDLLKKAHVRTIIEYNNKFVKRTLNPDKGHRFLPYIVVIIDEFGDLIMTAG
                     KDIELPIARIAQLARAVGIHMIIATQRPSTNIITGSIKANFPARVAFKVSSMIDSRTI
                     LDCPGANQLIGRGDMLISQGSDLVRIQCAFVDTPEIDNICKHIEGQRGFPSPFYLPEA
                     DVSNGADTDMSGVDLTQRDPMFEEAARLLVIHQQGSTSMIQRKFSIGYNRAGRIVDQL
                     EAAGILGPPDGSKPRQLLVQDEIQLERILLNLK"
BASE COUNT          487 a          320 c          286 g          577 t
ORIGIN      
        1 atttactaat gatccgtgag aaaggtgtcc tccgtgtgat aagtttaacc ctaaaaattt
       61 atctctagga ttcatacatg ctaaaaatac agcagcgttg gcttgtgcac cagagtgggg
      121 ttgtacgttt gcccattcag ctccaaaaag ctctttcaat ctatctattg ctaattgctc
      181 acttaagtct actacttcgc aaccgccgta gtatcgttta cctgggtagc cttcggcata
      241 tttatttgtt aagcaagaac ccattgcttc cattacttgt tcacttacaa aattttcaga
      301 tgcaataagc tcaatacctt ttagttgacg ctttttttct ttttcgatta tctcgaaaat
      361 ggctgtgtct cttttcatgt ttgtaattat gttattttaa atttaataag atacgttcta
      421 attgaatttc atcttgtacc aataattggc gaggtttact tccatctgga ggacctaata
      481 tccctgctgc ttctaattga tcaacaattc taccagcacg attatatccg attgaaaatt
      541 ttcgttgaat catcgaagtt gagccttgtt gatgaataac aagtagtctg gctgcttctt
      601 caaacatagg gtctcgttga gttaaatcca caccactcat atctgtatca gcaccattcg
      661 atacgtctgc ttctggtaaa taaaatgggc taggaaatcc tcgctgacct tctatatgtt
      721 tgcaaatatt atcaatttct ggagtatcaa caaaagcaca ttgaatgcga accaaatcgc
      781 taccttgcga aattagcata tctccacgcc caattaattg attagcacct gggcaatcca
      841 aaatagtacg agagtcaatc atcgaagata ctttaaaggc aacccttgca gggaagttcg
      901 cttttatact tcccgtaatg atgttggtcg aagggcgttg tgtagcaata atcatatgaa
      961 ttccaactgc tctagctaat tgggctatac gtgcaatggg taattcaatg tctttaccgg
     1021 ctgtcattat taaatcacca aattcgtcaa taataacaac gatatagggt aaaaatcggt
     1081 gtcctttatc tgggttgagt gtacgtttta caaacttatt gttgtattca ataatagtac
     1141 gtacatgtgc tttttttaat aagtcataac gactatccat ctctttggtc aacgaattca
     1201 gtgtttgtac taccttcgtt acatcggtaa tgatagcttc gtcggcatca ggcaatttag
     1261 caagaaagta cttttcaata tttgagtaaa tactaaactc tactttttta gggtcaatta
     1321 aaacaaattt taattgcgaa gggtgtttct tgtaaagtaa tgatgttata atcgcattta
     1381 gtcctactga ttttccttgt cctgttgctc ctgccactaa tatatggggc attttgcaca
     1441 aatcaacagt aaatacctcg tttgtaatgg tttttccaaa tacaattggc agttcatgtt
     1501 tagcttcttg aaacttttta gaagcaataa cagaatgcat tgatactatt tgtggtgttt
     1561 tgtttggtac ttcaatacca atagtacctt tgcctggaat aggagcaata atacgaatac
     1621 ctaaagcaga caaactcaat gcaatatcat cttctaaatt gcgaattttt
//
LOCUS       DAOPUR010000346         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_81159_length_1669_cov_2.454151, whole genome shotgun sequence.
ACCESSION   DAOPUR010000346 DAOPUR010000000
VERSION     DAOPUR010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81159_length_1669_cov_2.454151"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="PKN90_06195"
     CDS             complement(<1..105)
                     /locus_tag="PKN90_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL21 family ribosomal protein"
                     /protein_id="HNZ85899.1"
                     /translation="MYAIVEIAGQQFKVEKDRTLFVHRLNEERGATVEF"
     gene            complement(240..986)
                     /locus_tag="PKN90_06200"
     CDS             complement(240..986)
                     /locus_tag="PKN90_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ85900.1"
                     /translation="MEVIFLGTGTSTGVPYINCPCDVCQSTDSKDKRLRSSVLVSVDT
                     KEILIDCGPDFRQQAIVNDISTISAVLLTHEHFDHVTGIDDLRAYGEVEIYAEERVCQ
                     SLKRTIPYCFGEIKYPGVPLISLHEIDEMPFDLHNTEIVPIRIWHHKLPILGFRIGAF
                     AYLTDISGIDDKEYEKLQNLDVLVVDALRLKPHFSHFTLEQAISFAEKTNAKEVYFTH
                     ISHQLGKHDEIQSLLPHNMMLAYDGLKITI"
     gene            complement(993..>1669)
                     /locus_tag="PKN90_06205"
     CDS             complement(993..>1669)
                     /locus_tag="PKN90_06205"
                     /inference="COORDINATES: protein motif:HMM:NF025020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HNZ85901.1"
                     /translation="EKENEENITWLIQELGAEYIPIAIDNFPEITNTDVGYRFYPHKT
                     KGEGLFMALVQKKSACNKQNIKLKTDKNKQNTADIEALNLYINDSSDWIIQQNNQQYR
                     AILASKQQEIKWIEKHLYVLFSGIQLAEKKGNDFVPHEHLALSKQLNTNSFQVVDVDW
                     ITAIQFLKREALFLPDASKGYVLLQYNNVPVGWVKNIGNRCNNMYPSSWKVRLDISHF
                     TNSQII"
BASE COUNT          500 a          290 c          269 g          610 t
ORIGIN      
        1 aaattctaca gttgcacctc tttcttcgtt taagcgatga acaaacaatg ttctgtcttt
       61 ttcaacttta aattgttgtc ccgcaatttc tacaatagcg tatatagtta taaattaatt
      121 ataaaatacc ttcgtgaggt gaatgcttta gctgcatttc ttttctacct ttcagaattt
      181 gagtggcaaa gatactatat aattttattc tgtacaagta atttatattc ttttttgtgt
      241 catatagtga ttttcaaccc atcgtatgcc aacatcatat tgtgaggaag taaactttga
      301 atctcatcat gtttgcccaa ctgatggcta atgtgtgtaa aatacacttc tttagcattt
      361 gttttttctg caaaactgat ggcttgttct aaggtaaagt gagaaaaatg cggtttaagg
      421 cgtagtgcat ctactactaa tacatccaaa ttttggagtt tttcgtattc tttgtcatcg
      481 atcccactaa tgtcggttaa ataagcaaaa gcacctattc taaaacctaa aataggtaat
      541 ttatgatgcc aaatacgtat cggaacaatc tccgtattgt gtaaatcaaa aggcatttca
      601 tcaatttcgt gtaacgaaat aagaggaact cctgggtatt taatttcgcc aaaacaatac
      661 ggtatggttc gttttaatga ttgacacact cgctcttctg cataaatttc tacttcgcca
      721 taggctcgta aatcatctat gccagtaacg tggtcaaaat gctcgtgtgt aagcagaaca
      781 gctgatattg tggaaatatc attaacaatg gcttgctgac gaaaatcagg tccacaatct
      841 atcaatattt ctttcgtatc gactgaaact aaaacagagg atcgtaatcg cttatcttta
      901 ctatcagtag attgacatac atcgcaagga caattaatat atggaacacc agtagatgtg
      961 cctgttccta aaaatatgac ttccattatg gtttatatga tttgactgtt tgtaaaatgt
     1021 gaaatatcca aacgtacttt ccacgaactt ggatacatat tattacacct gttgcctata
     1081 tttttgaccc atcctactgg tacattattg tattgcaaca acacataacc ttttgatgcg
     1141 tcgggtaaaa acaaggcttc tcgtttaaga aattgaatgg ctgttatcca atcaacatct
     1201 actacttgaa aagaattagt attaagttgt ttagaaaggg ctaagtgctc gtgaggaaca
     1261 aagtcattgc cttttttttc agccagttga attcctgaaa acaatacata taagtgtttt
     1321 tcaatccatt taatttcttg ttgtttggag gctaatattg ctctgtactg ttggttattt
     1381 tgctgtatta tccaatctga cgaatcgttt atgtataaat taagagcctc aatatcagca
     1441 gtattttgtt tattcttgtc tgtttttagt tttatatttt gtttattaca agcactcttt
     1501 ttttgaacta atgccataaa caagccttct ccttttgttt tgtgtggata aaaacgataa
     1561 ccaacatctg tatttgttat ttcagggaag ttatcgatgg caatcggtat atattcagca
     1621 cccaattctt gtatcaacca tgtaatgttt tcttcgtttt ctttttcat
//
LOCUS       DAOPUR010000347         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_82169_length_1655_cov_3.123750, whole genome shotgun sequence.
ACCESSION   DAOPUR010000347 DAOPUR010000000
VERSION     DAOPUR010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82169_length_1655_cov_3.123750"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKN90_06210"
     CDS             complement(<1..277)
                     /locus_tag="PKN90_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85902.1"
                     /translation="MKLKWLTNAFLLLLNSIVLVILLVINYVSVLSPDSVWSVLAPTN
                     ILFTAFVSINLLFVLWWIIHLKWYAIFSVLALLLCYTNISNTFIIWSE"
     gene            complement(293..1177)
                     /locus_tag="PKN90_06215"
     CDS             complement(293..1177)
                     /locus_tag="PKN90_06215"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ85903.1"
                     /translation="MWSQIKTNFKQGSYLTRLLYINLGMFVLVRLVLLIFTLFKHDIS
                     FAIRYIEMPAYIGNWITQPWVIITYMFLHVDFIHFIFNVLVLYWYGTIFLQFFNQKQL
                     VGVYILGGIGGAFLYVFGYSVFPYFQEVLKHTYLLGASASVMALIFTTVIYAPNYEVQ
                     LALIGRVKLKYIGLVLFAVDVLSVTSVNAGGSIAHIGGAITGILFASLYVRQRVDLTT
                     GISWLLDKLATLVQPKPKITVSYGRPKTDAEWNVEKKAKNVALDEILEQIKKSGYDNL
                     SAEQKKMLFDISNKKNNN"
     gene            complement(1208..>1655)
                     /locus_tag="PKN90_06220"
     CDS             complement(1208..>1655)
                     /locus_tag="PKN90_06220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ85904.1"
                     /translation="IGAAIVQQITWSFDFVPIANAFGEYVNNRDISVLMPYLNVNEST
                     VLSTTEVLQLKNMVLNQPITVGASGAVFGILLAFAMLFPDARIMLLFLPVPIKARYFV
                     IFYGAMELFLGVSNFQGDSIAHFAHLGGMLFGLVLILLWRKKGTLY"
BASE COUNT          616 a          319 c          241 g          479 t
ORIGIN      
        1 tttcagacca aataataaac gtattactaa tatttgtata acaaaggagt agggcaagaa
       61 cagaaaaaat agcataccac ttcaaatgga taatccacca tagtacaaac aataaattga
      121 tactaacaaa ggctgtgaaa agaatgttag taggagccaa tactgaccat accgagtcgg
      181 gagacaatac agaaacataa ttgattacta acaaaatcac taatacaatg ctattgagca
      241 ataaaagaaa tgcatttgtc aaccacttga gtttcatgtt ggtcggtatt ttttagttat
      301 tgtttttttt attgctaatg tcaaacagca tttttttttg ttcagcactc aaattatcgt
      361 aacctgattt ttttatttgt tctaaaattt catcaagagc tacattttta gctttttttt
      421 ctacattcca ttcggcatct gttttcggtc tgccatagct tacggtaatt tttggttttg
      481 gttgcactaa ggtagctagt ttatcaagta accaactaat gcccgtagtt aaatcgactc
      541 gttggcgaac atataaacta gcaaacaata tgcccgtaat ggcacctcca atgtgagcaa
      601 tactccctcc agcatttact gaggttacac ttaatacatc tactgcaaat aaaactaaac
      661 caatgtattt caatttaaca cgcccaatta aagcaagttg cacttcataa ttaggagcat
      721 atatcaccgt agtaaaaatc agagccatca ccgatgccga agcacctagc aaatacgtat
      781 gtttcaatac ttcttggaaa taaggaaaca cagaataccc aaaaacatat aggaatgcac
      841 caccaatacc tcccaatata tacacaccta ctaactgttt ttggttgaaa aattgcaaaa
      901 aaatggtgcc ataccagtac aataccaaaa cattaaatat aaagtgaata aaatctacat
      961 gcaaaaacat ataggtaatg atgacccatg gctgtgtgat ccaatttcct atatatgcag
     1021 gcatttctat atatcgtata gcaaaactga tatcatgttt gaacaaggta aaaatcaaca
     1081 ataccaaacg aaccaataca aacatgccca agttgatata caacaagcgt gtaagataag
     1141 agccttgttt aaaattagtt tttatttgag accacatagt catttttttt ctttagtgtt
     1201 taaaccatta gtataaggta ccttttttac gccaaagcag aattaacact aagccgaata
     1261 acataccccc caaatgagca aaatgagcta tactatctcc ttgaaaatta cttacaccta
     1321 aaaacaattc catagcacca taaaaaataa caaagtatct tgccttaatg ggcacaggta
     1381 aaaataagag cataatacgt gcatcaggaa ataacatagc aaacgcaagc aatataccaa
     1441 acactgcacc tgaagctcca actgtaatag gctggttcag taccatattt tttagttgca
     1501 acacttcagt ggttgacaat acggtagatt catttacatt taagtaaggc attaatacac
     1561 taatatcacg attatttaca tattcgccaa aggcattagc aataggaaca aaatcaaacg
     1621 accatgttat ttgttgcaca atggcagctc caata
//
LOCUS       DAOPUR010000348         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_82879_length_1646_cov_4.114393, whole genome shotgun sequence.
ACCESSION   DAOPUR010000348 DAOPUR010000000
VERSION     DAOPUR010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82879_length_1646_cov_4.114393"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PKN90_06225"
     CDS             complement(<1..1192)
                     /locus_tag="PKN90_06225"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNZ85905.1"
                     /translation="MDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALESLGVDVIEA
                     GFPVSSPGDFKSVVEISKAVTWPTICALTRGVQKDIEVAAEALQYAKNKRIHTGIGTS
                     DYHIKHKFNSTREEILERAVAAVKYAKKYVEDVEFYCEDAGRSDNEYLARVVEAVIKA
                     GATVVNIPDTTGYCLPSQYGEKIKFLMENVKGVHNAILSTHCHNDLGMATANTMAGVL
                     NGARQVEVTMNGIGERAGNTSLEEIAMILKCHKHLEIDTQINTQKIASTSRLVSSLMN
                     MPIQPNKAIVGRNAFAHSSGIHQDGVLKNRESYEIMDPKDVGIDENAIVLTARSGRAA
                     LKHRLSILGVAVEGDALDKIYQDFLVLADKKKDVNDDDVLMLAGMERTSNSRIKLDYL
                     QVTSG"
BASE COUNT          502 a          337 c          239 g          568 t
ORIGIN      
        1 ttcctgatgt tacttgtaaa taatcaagtt taatacggct attgcttgtg cgttccatac
       61 cagccaacat caatacgtca tcatcgttca cgtctttttt cttatcggca agcactaaaa
      121 agtcttggta aattttgtcc aaggcatcgc cttctacagc aacacccaat atacttaaac
      181 gatgtttaag agcagcacga ccactacgag cagttaaaac aattgcattt tcatcaattc
      241 ccacatcttt aggatccata atctcatagc tctctctgtt cttcaacaca ccatcttggt
      301 gaatacctga tgaatgtgca aaggcattac gacccacaat cgctttgttg ggttgtatag
      361 gcatattcat taaactagac accaaacgac tagtgcttgc aattttttgt gtgttaattt
      421 gggtatctat ttctaaatgt ttgtggcatt tcaaaatcat tgctatttct tctaatgagg
      481 tgttaccagc tctttcgcca ataccattca tggttacttc tacctgacgt gctccgttta
      541 ataccccagc cattgtatta gcagtagcca ttcctaagtc gttgtggcaa tgagtagaaa
      601 ggatagcatt atgaactcct tttacatttt ccatcaaaaa tttaattttc tctccgtatt
      661 gtgatggcaa acaatagcct gttgtatcag gaatgttaac cacagttgct ccagctttga
      721 ttacagcctc tacaactctt gctaagtatt cgttatcact acgaccagca tcttcgcaat
      781 aaaattccac atcttccaca tacttcttgg catattttac agcagcaaca gcacgttcta
      841 atatttcttc gcgtgtagaa ttaaatttgt gttttatatg ataatctgat gtaccaatac
      901 ctgtatgaat acgtttattt ttagcgtatt gtaaagcttc agcagctact tcaatgtctt
      961 tttgtacacc acgtgtaagt gcacaaattg tgggccacgt tacagctttg gatatttcta
     1021 ccactgattt aaagtctcct gggctagata ctggaaatcc tgcttcaatt acatctacac
     1081 ctaaagattc taaggctttt gctacctgaa ttttttctac tgtattcaac tgacaccctg
     1141 gcacttgttc tccatctctc aaggtggtgt caaaaataaa taatctgtcc atatcctcac
     1201 gtatttatat tgttcttaca aaaattacaa gaaaaagcct ctcattaccg aagtaagaaa
     1261 ggctttctat gttattatta attttccaca taccaaactc actccgctca tcctttacag
     1321 agaagaatac taagtaaaat agtatgtaga gtgctaaatg acataatcgt tgttttgttt
     1381 ggcaaagata ctgtcttttt tttattcaaa caaactattt aaaagaaaaa tattaaaaaa
     1441 aatgtcaaaa aacaactgta tctttctaaa taaaatcaaa tataaaaaat aatgcacatg
     1501 attaaacctt tatttttttt cttcataagg ttctgtatgt atattcacaa ctgtttgatt
     1561 accatattga tgacgcaaca aagattctac gtctgtagca atatcatgag actctacaaa
     1621 acttatatgt tgttcagttt aatatg
//
LOCUS       DAOPUR010000349         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_83280_length_1641_cov_2.824086, whole genome shotgun sequence.
ACCESSION   DAOPUR010000349 DAOPUR010000000
VERSION     DAOPUR010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83280_length_1641_cov_2.824086"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PKN90_06230"
     CDS             <1..1293
                     /locus_tag="PKN90_06230"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNZ85906.1"
                     /translation="NDPLIDNKDSKKRYRADVLIEDLMAKYDDKINKEIEKAAKKFGD
                     AFDATLFAETNPRIQENKAKKEEIHTRFAKALNDGDLNELKQIIVDYDVVCPISGTKN
                     WTDVRQFNLMFSTEMGSTADGAMKIYLRPETAQGIFVNYLNVQKTGRMKIPFGIAQIG
                     KAFRNEIVARQFIFRMREFEQMEMQFFVRPGEEMKWFEHWKQFRLRWHKALGLGDHKY
                     RFHDHDKLAHYANAATDIEFELPFGFKEIEGIHSRTDFDLGSHEKYSGKNIKYFDPEL
                     NQSYTPYVVETSIGLDRTFLSIMATAYCEETLEGNETRVILKLPPALAPIKVCIMPLV
                     KKDGLPEKAREIMDNLKFEFKCSYDEKDSIGKRYRRQDAIGTPFCITIDHQTLEDNCV
                     TIRHRDSMQQERVAIDSLETIVGKAVSMKKMLQSIVQD"
     gene            1407..>1641
                     /locus_tag="PKN90_06235"
     CDS             1407..>1641
                     /locus_tag="PKN90_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNZ85907.1"
                     /translation="MKEELLHHIWKYRLFASHQLTDTEGSTIDIIDVGHHNLDAGPDF
                     INAKIKIGDTLWAGNVEIHKKASDWNTHKHPTNP"
BASE COUNT          556 a          313 c          333 g          439 t
ORIGIN      
        1 aacgatccgt tgattgacaa taaggacagc aagaaacgct accgtgccga tgtgttaatc
       61 gaagatttga tggcaaaata tgacgataaa atcaacaaag aaattgaaaa agctgccaag
      121 aaatttggcg atgcgtttga tgctaccttg tttgccgaaa ccaatcctcg catacaagaa
      181 aataaagcaa aaaaagaaga aattcataca cgttttgcaa aagccttgaa cgatggcgac
      241 ctcaacgaat tgaaacaaat tattgttgat tacgatgttg tgtgccctat tagtggcact
      301 aaaaactgga ctgatgtgcg tcagtttaac ttgatgttta gcaccgaaat gggttcgacg
      361 gcagatggtg caatgaaaat atatttacgc cccgaaacag cacaaggtat ttttgtaaac
      421 tacctaaacg tgcaaaaaac gggtagaatg aaaattcctt ttggtatagc tcaaataggt
      481 aaagcattcc gcaacgagat tgtagctcgt cagtttattt tccgtatgcg tgaatttgaa
      541 caaatggaaa tgcaattttt tgtgcgtcca ggtgaggaaa tgaaatggtt tgagcattgg
      601 aaacaattcc gtttgcgttg gcacaaagcc cttggattgg gcgaccataa ataccgtttt
      661 cacgaccacg acaaattagc gcattacgcc aatgctgcta ctgatattga atttgaatta
      721 ccattcggtt tcaaagaaat tgaaggcata cactctcgta cagattttga cttgggtagc
      781 cacgaaaaat attcgggtaa aaacatcaag tatttcgatc ctgaacttaa ccaatcgtac
      841 acgccttatg tggttgaaac ctcaatagga ttagatagaa ccttcctttc gataatggct
      901 actgcttatt gcgaagaaac gttagagggg aacgaaacgc gtgtgatact aaaattgccg
      961 ccagccttag ctcctatcaa ggtttgtatt atgcctttgg taaaaaaaga tggtctgcct
     1021 gaaaaagcac gtgaaattat ggacaatttg aaatttgaat tcaaatgttc gtatgacgag
     1081 aaagattcta ttggtaaacg ttatcgtcgt caagatgcca ttggtacacc tttctgcatt
     1141 accatcgacc accaaacttt ggaagacaac tgcgtaacca tacgtcatcg cgatagcatg
     1201 caacaagaac gtgttgccat tgatagccta gaaactattg ttggcaaagc tgtaagcatg
     1261 aaaaaaatgc tacaatcaat cgttcaagat taaaccttta ctacctcctt tttgtctaac
     1321 acataaagga ggttttttta taccctatcc gattaccaat taatctggtc gtgaatgtga
     1381 gcaaacaacg ataaaaaagc aagcacatga aggaagaact tttgcatcat atctggaaat
     1441 atcggttatt tgctagccat caacttaccg acacagaagg gtcaacgata gatataattg
     1501 atgtagggca tcacaacttg gatgctggtc cggattttat caacgcaaaa ataaagattg
     1561 gcgacaccct ttgggctggc aatgtagaaa ttcataaaaa agcgtccgat tggaatacac
     1621 acaaacaccc aacaaatcca g
//
LOCUS       DAOPUR010000350         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_83749_length_1635_cov_2.787975, whole genome shotgun sequence.
ACCESSION   DAOPUR010000350 DAOPUR010000000
VERSION     DAOPUR010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83749_length_1635_cov_2.787975"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1020)
                     /gene="rfbD"
                     /locus_tag="PKN90_06240"
     CDS             complement(139..1020)
                     /gene="rfbD"
                     /locus_tag="PKN90_06240"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNZ85908.1"
                     /translation="MTNVLITGAYGQLGSELKKLAPQYTQFTYFFTDVDTLDICNKQA
                     ILQFCIDNSIHFILNCAAYTAVDKAEEEEQHSLCYAINAEAVKNLGEVASQIGANIIH
                     ISTDYVFDGKAYLPYTEDITTNPQSLYGKSKLLGEQWLMKSCAHALIIRTSWLYSTFG
                     NNFVKTMIKLGTERTELKVIFDQIGTPTYAADLANTMLTIVAKSTEKATNYVPGIYHF
                     SNEGVCSWYDFTIAIHALYGISTCNVLPIESKEYPTKAPRPHYSVLNKQKIKTTFDIT
                     IPHWQTSLANCIGKLKT"
     gene            complement(1038..1592)
                     /gene="rfbC"
                     /locus_tag="PKN90_06245"
     CDS             complement(1038..1592)
                     /gene="rfbC"
                     /locus_tag="PKN90_06245"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNZ85909.1"
                     /translation="MEIIETKIPGLYIVQPKVFGDHRGYFCETYNEKTFHSLGITNKF
                     CQDNLSKSSYGVIRGLHFQLHPHSQAKLVSAVVGTVYDVAVDIRVGSPTYGEWVGIEL
                     SEENKTQFLIPQGFAHGFSVLSETAIFSYKCDNLYTPSAEGGILYNDPTLQIDWKVPF
                     EKQLVSDKDTKHPTLKDVKTNFVF"
BASE COUNT          499 a          266 c          297 g          573 t
ORIGIN      
        1 ggttagtgtt tctcgttaat tgtaaaaaga acgcccaaag ttactgtaaa aaaagaacta
       61 tacaaattat ttaacattct ttttttattt tatttaccaa ggtaaaatgt gcttactttg
      121 agcaaggcat cgcgattatt acgtctttaa tttacctata caattggcta acgatgtttg
      181 ccaatgagga atagtaatgt caaaagtggt ctttattttc tgtttgttaa ggacactata
      241 atgcggacgg ggagctttgg ttggatattc tttactttca ataggtaaca cattacaggt
      301 agaaatgcca tataaagcat gtatagcaat tgtgaagtcg taccaactac atacgccttc
      361 gttagagaaa tgatatatgc caggcacgta attggtagct ttttctgttg atttggctac
      421 tatggttaac attgtatttg ctaaatcagc agcataggta ggggtgccaa tttgatcaaa
      481 aatcaccttg agttccgtac gttctgtacc taactttatc attgttttta caaaattgtt
      541 tccaaaagta gaatatagcc atgaggtacg aataataagt gcatgagcac atgatttcat
      601 tagccattgt tctcctaata gttttgattt tccatacaat gattgaggat tagtcgttat
      661 atcttcagtg tatgggagat atgctttgcc atcaaataca taatcagtag aaatatggat
      721 tatattggct cctatttgcg aagccacttc tcctaagttt tttacagctt cagcattgat
      781 agcataacac aaagagtgtt gctcttcttc ttccgcttta tctacagcag tgtatgcggc
      841 acaatttaaa atgaaatgaa tagaattatc tatgcaaaac tgtaaaattg cttgtttatt
      901 acatatatca agcgtatcaa catccgtaaa aaaataggta aactgtgtat attggggtgc
      961 aagtttttta agttcgcttc ctagctgacc ataagctccg gttattaata cgtttgtcat
     1021 acgattcaat tttataatta aaatacaaaa ttagttttca catccttcaa ggtagggtgt
     1081 tttgtgtctt tgtctgaaac aagttgtttt tcaaaaggaa ctttccagtc tatttgtaag
     1141 gttggatcgt tatataaaat acctccttct gctgatggtg tatatagatt gtcacatttg
     1201 taagagaaaa tagccgtttc gctaagaact gaaaatccat gtgcaaatcc ttgtgggata
     1261 agaaattgtg ttttattttc ttcgcttaat tcgataccta cccactcgcc atatgtaggc
     1321 gaaccaacac gtatatctac agctacatca tataccgtgc ccactactgc cgaaactagt
     1381 tttgcttgac tgtgtgggtg tagctgaaaa tgtaaaccgc gaataactcc gtacgatgat
     1441 ttagacaaat tgtcttgaca aaatttgttt gtgatgccca aagagtgaaa tgttttttcg
     1501 ttatatgttt cgcaaaaata acctctatga tcgccaaata ctttgggttg aacaatatat
     1561 aaacctggga ttttcgtttc aataatttcc atgtgtacgt ttttgatagt aaaaaaggat
     1621 gttatttttg agatg
//
LOCUS       DAOPUR010000351         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_84049_length_1631_cov_7.667513, whole genome shotgun sequence.
ACCESSION   DAOPUR010000351 DAOPUR010000000
VERSION     DAOPUR010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84049_length_1631_cov_7.667513"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="PKN90_06250"
     CDS             complement(<1..1496)
                     /locus_tag="PKN90_06250"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HNZ85910.1"
                     /translation="MQGRKELTPKMLYQVHLQDLIPEHNFYRLLDKAIDFHFLYKSTA
                     QYYGEEGQESIDPVVFFKICLVGYLNNINSDRKLIEYCSNCLDVRLFIRYDIDEALPW
                     HSTISRTRQLYGEEVFLTLFKAVLKLCVSKGMIRGKRQAVDSVFIKANASMDSLVEKE
                     VLDDASAFVDELEENSEFKTTSTRKKLVEQHHAWKEEAYKNQPNPNFNIDKVDEHGNL
                     IRPRFVSNHTHYSPTDSDARVSVKPGKARQLNYFGQIVVDDAHHVITGACSDFADKRD
                     SQCLEKIVELTEQNLSENGIELEELLADGGYSSGEALKYLNQKNINAYIPNFGQYKPE
                     REGFIFNKELHQYECIKEGSNKAVLLFKGEKIDSKGYTKRSYRSSESDCKNCPLREQC
                     CGKTTKYKKIDDSIHKEHYDRMHLKLTQNPNYAKKMVRVRSKTVEPVIGTLVNFTNMK
                     RVNTKGIKQANKHVLMAALTYNLKKYLKFIAKKTKTNAGVVSEIQTKAP"
BASE COUNT          419 a          304 c          283 g          625 t
ORIGIN      
        1 ggtgcttttg tttgtatttc acttacaact ccagcatttg tttttgtttt tttggcgata
       61 aacttcaaat atttcttcaa attataggtt aatgctgcca tcaaaacgtg tttattggct
      121 tgtttgattc cttttgtgtt tacacgcttc atattggtaa aattgaccaa ggttccaatt
      181 acaggttcta ccgttttact tcttactcgt accattttct tggcatagtt tgggttttgg
      241 gtgagtttta agtgcattct atcgtagtgt tctttgtgga tgctgtcgtc gatctttttg
      301 tatttggttg tttttccgca gcattgttct cgtagtggac agtttttgca atcgctctca
      361 ctactgcgat agctgcgttt ggtgtagcct ttgctatcga tcttttcgcc tttaaatagt
      421 aaaacagcct tgttggaacc ttctttgata cattcgtact gatgtaattc tttgttaaaa
      481 ataaatcctt ctctctcagg tttgtattgt ccaaagtttg gaatataggc attgatgttt
      541 ttttggttca aatactttaa agcttctcca ctactataac ctccatcggc taagagttct
      601 tctaattcaa tgccgttttc acttaggttt tgctcagtta gttcaactat tttttctaaa
      661 cattgactat cgcgtttgtc tgcaaaatca gaacaagctc ctgttattac gtgatgagcg
      721 tcgtctacaa cgatttgtcc aaaatagttt aattgtcttg ctttgcctgg ttttacactt
      781 accctggcat cagaatctgt gggggaataa tgcgtatgat tcgatacaaa tctcggacgt
      841 attaaattac cgtgttcatc tactttatcg atgttgaagt tgggattggg ttggttttta
      901 taagcttcct ctttccaggc gtggtgttgc tctactaatt tctttctcgt gcttgtagtt
      961 ttaaattcac tattttcttc taattcatct acaaaagcac tggcatcatc caatacttct
     1021 ttttctacca aactgtccat agaagcattg gctttgataa aaacgctatc cacggcttga
     1081 cgtttgcctc gaatcatccc tttggaaaca catagtttta aaacggcttt gaataaggtt
     1141 aaaaacactt cttctccata caactgacgg gttctactaa tggtgctgtg ccaaggcaat
     1201 gcttcatcaa tgtcataccg aataaacagg cgaacatcta aacaattgga gcaatattcg
     1261 atgagttttc tatccgaatt aatattgttt agataaccca ccaaacagat tttaaaaaac
     1321 acaacaggat caatactttc ttgtccttcc tctccataat actgggctgt ggatttatat
     1381 aaaaaatgaa aatcgatagc tttatctagc aatcgataaa aattatgctc aggaatcaag
     1441 tcctgtaaat gaacttgata gagcattttt ggcgtgagtt cttttcttcc ttgcatagct
     1501 aaaaatacta ttttatttac ctttttgcaa gttttttttt gatatatttg ttgtgttgtg
     1561 caacaagcac atcggtttgg caagattgcg aattttgtag taaatacacg tttacatttc
     1621 gcaagaaatt t
//
LOCUS       DAOPUR010000352         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_84067_length_1631_cov_3.348350, whole genome shotgun sequence.
ACCESSION   DAOPUR010000352 DAOPUR010000000
VERSION     DAOPUR010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84067_length_1631_cov_3.348350"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKN90_06255"
     CDS             <1..834
                     /locus_tag="PKN90_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HNZ85911.1"
                     /translation="YLTCDHQLMLEKAEEIIVGIQILMTVLQVNKAIIGIENNKKDAI
                     SLLQQITSSYSTIEVCPLAMCYPQGGEKQLIEAITKKQVPSGQLPINIGIVVQNVATT
                     YAIYEAVQKNKPLIERVITVTGKKVAKPGNFLVRIGTPLRVPLDMAGGMPEDTGKIIG
                     GGPMMGRAFMNADVPVTKRSAGLLVMPTLESARKEMQNCIRCAKCVTACPMGLEPYLL
                     MSQAEKSMWKEMEETYITDCIECGCCLFSCPSNRPLLDYLRMGKSTVLEQIRNRVQKV
                     N"
     gene            856..>1631
                     /locus_tag="PKN90_06260"
     CDS             856..>1631
                     /locus_tag="PKN90_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNZ85912.1"
                     /translation="MNKLIISPAPHIHSGDSISKNMYAVLLALVPAYLVGLYFFGIGA
                     FVVSITAVLSCIIFEFLIQKFIFKTTTTIHDGSAMLTGLLLAFNLPSNLPIWIIIIGA
                     LVAIGIGKMSFGGLGNNPFNPALVGRVFLLISFPVQMTTWPKPIPFNTSYLDAETAAT
                     PLAMIKYNFGNLPEKMDLLIGNMGGSIGEVSAIALILGGIYLLIKRVITWHIPVSMLV
                     TTFLFTGILHLINPSLYVSPDIHLLTGGLLLGAIFMATDYA"
BASE COUNT          511 a          267 c          306 g          547 t
ORIGIN      
        1 tatttaactt gcgaccatca attaatgctc gaaaaagcag aagaaataat tgtaggtatt
       61 caaattttaa tgactgtatt gcaagtaaac aaagccatta ttggcataga aaataacaaa
      121 aaagatgcca tttctctatt acagcaaatc acaagttcat attcaactat tgaagtttgc
      181 cccttagcaa tgtgttaccc acagggaggc gaaaagcaac taattgaggc aatcacaaaa
      241 aaacaagtgc caagtggtca attacctatt aatattggta ttgtcgttca gaatgtggca
      301 acaacatatg ccatttacga agcagttcaa aaaaacaaac ctttaataga acgtgtaata
      361 acagttacgg ggaaaaaagt tgcaaaacca ggtaattttt tagtacgtat tggaactcct
      421 ttacgcgttc cattagatat ggctggggga atgcccgaag atactggaaa aattataggt
      481 ggaggtccaa tgatgggacg agcttttatg aacgcagatg tacctgttac gaaacgtagt
      541 gctggtttat tagtaatgcc tacccttgag tctgcccgta aagaaatgca aaattgcatt
      601 cgttgtgcca aatgtgtaac agcttgtcca atgggtctag aaccctattt attgatgtct
      661 caggctgaaa aaagcatgtg gaaagaaatg gaagaaacgt acattactga ttgtattgaa
      721 tgtggttgct gtttgttttc ttgtcctagt aatcgaccac tattagacta tctacgtatg
      781 gggaaaagca ctgttttgga acaaatacgt aatcgcgttc aaaaagttaa ctaattgaaa
      841 tctacacaaa gtattatgaa taaactaatt atttcgccag ctccacatat acatagtggc
      901 gattctattt ctaaaaatat gtatgcagta ctacttgcat tagtacctgc ctatttagtt
      961 ggtttgtatt tttttggaat tggtgctttt gttgtcagta ttactgccgt tttatcttgt
     1021 attatttttg aatttttaat tcaaaaattt attttcaaaa ctaccactac tatccatgat
     1081 ggttctgcca tgcttacggg cttattatta gcttttaacc tcccatctaa cctacccata
     1141 tggattatta ttattggagc attagtagcg ataggtattg gtaaaatgag ttttggagga
     1201 ttaggtaaca atccatttaa tccagcttta gttggtcgtg tatttttatt aatatctttt
     1261 cctgtacaaa tgacaacttg gcccaaacca ataccattta atacaagtta tttggatgct
     1321 gagactgctg ctacaccttt ggctatgatt aaatataact ttggtaattt accagaaaaa
     1381 atggacttac tcattggtaa tatgggtggt agtattggag aggtatctgc tatcgcatta
     1441 attctaggag gaatctattt gctgatcaaa cgtgttatta catggcatat cccagtaagt
     1501 atgttagtta ctacattttt gtttacaggg atattgcatt tgattaatcc ttctttatat
     1561 gtatctcccg acattcacct gttaactgga ggattgctat tgggagctat ttttatggct
     1621 actgattatg c
//
LOCUS       DAOPUR010000353         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_84096_length_1631_cov_2.439721, whole genome shotgun sequence.
ACCESSION   DAOPUR010000353 DAOPUR010000000
VERSION     DAOPUR010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84096_length_1631_cov_2.439721"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="nth"
                     /locus_tag="PKN90_06265"
     CDS             <1..540
                     /gene="nth"
                     /locus_tag="PKN90_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNZ85913.1"
                     /translation="ELLIAIILSAQCTDKRVNLTTPALFKSFPTPQAMAKASFEEVFA
                     LIKSISYPNNKAKHLIALSQQLVDKYDGVIPDDAERLQELPGVGRKTANVMLVVAFKK
                     PAMPVDTHVFRVANRIGLTTAKNPLQSEKTLVKNIPNHLLPIAHHWLILHGRYTCLAR
                     KPKCDECGIRTFCKFFNKK"
     gene            601..>1631
                     /locus_tag="PKN90_06270"
     CDS             601..>1631
                     /locus_tag="PKN90_06270"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNZ85914.1"
                     /translation="MSLNIENILLIGSLLLLVSIIAGKTSYRFGVPTLILFLAVGMLS
                     GSEGLGGIRFDDPQIAQFIGIVSLNFILFSGGLDTNWHQVKPVLWQGITLSTVGVLIT
                     AISLGTFVWLITDFSLVESLLLGSIVSSTDAAAVFSILRSKSLSLKKNLRPTLQLESG
                     SNDPMAYVLTIAFLTLVVHQDMSIVSIIPLFLQQMVFGAILGYVFGRISKLIINRIKL
                     DFEGLYPVLVIALMFLTFSATNFVGGNGFLAIYICAVYLGNQNLIHKRTILKVYDGLA
                     WLMQIVLFLTLGLLVFPSHILPVVGIGLLVSVFLIIIARPLSVFLSLLPFRMPIRSRF
                     YISWVGLRGA"
BASE COUNT          440 a          264 c          317 g          610 t
ORIGIN      
        1 gagttattaa ttgctattat tctttctgcc caatgtacag ataaacgggt aaatttaaca
       61 actccggctt tatttaagtc gtttcctact ccacaagcta tggctaaagc ttcttttgaa
      121 gaggtttttg ctcttatcaa aagtatttca tatccaaata acaaagcaaa acatctaata
      181 gctttatctc agcaattagt agataaatac gatggagtta taccagacga tgccgagcgt
      241 ttgcaagagt tgcctggtgt gggtcgcaaa acagccaatg tgatgcttgt agtagctttt
      301 aagaagccag ctatgcctgt agatacacat gtatttaggg tagcaaatag aattggttta
      361 actactgcaa aaaacccatt acaaagtgaa aagactttag taaagaatat tcccaaccac
      421 ttattaccta tagcacatca ttggcttatc ttgcatggtc gttatacctg tttggcacgc
      481 aaacccaaat gcgacgaatg tgggatacgc actttttgta agttctttaa caaaaagtaa
      541 aaaggtttct ttcggatttt ttatatcttt gtaggcatca tacttttatg cctgaaatct
      601 atgagtttga atatagaaaa tatcctgtta attgggtcgt tattgctctt agtaagtatt
      661 attgcaggta aaacatcgta tcgctttggt gtaccaacgt taattttatt cttagctgta
      721 gggatgttat ctggttctga gggattagga ggtatacgtt ttgatgaccc tcaaattgct
      781 caatttattg gcattgtttc attaaacttt attctttttt ctggcgggtt agataccaat
      841 tggcatcagg ttaagcctgt tttgtggcaa ggtatcacgc tttcgactgt aggagtttta
      901 attacagcaa tatcgttggg tacatttgtt tggctaatta ctgatttttc gttagtggaa
      961 agtttgctgt tggggtctat tgtgtcatca actgatgcag ctgctgtatt ttcaattttg
     1021 aggtcaaaaa gtttatctct taaaaagaat ttacgtccta ctcttcagct cgaaagtgga
     1081 agtaacgacc ctatggctta tgttttaacc attgcttttt taaccttggt tgttcatcaa
     1141 gatatgtcaa ttgtttctat tattcctctg tttttacagc aaatggtttt tggtgctatt
     1201 ttggggtatg ttttcggacg aataagcaag cttattatca atcgcataaa attagatttt
     1261 gaaggcttat atccagtgtt agtcattgcc ctgatgtttt taacgttttc ggcaactaat
     1321 tttgttggtg gaaatggttt ccttgcaata tacatatgtg ccgtttattt aggcaaccaa
     1381 aacttaatac acaagcgaac tatcttaaaa gtttatgatg gattggcgtg gttgatgcaa
     1441 attgtgcttt tcttaacatt aggactatta gtttttccaa gtcatatttt acctgttgtt
     1501 ggtattggat tgttggtatc agtattttta ataatcatag ctcgtccttt aagtgttttt
     1561 cttagcttgc ttccttttag aatgcccata cgtagccgtt tttatatttc ttgggttggc
     1621 ttacgaggtg c
//
LOCUS       DAOPUR010000354         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_84155_length_1630_cov_3.067302, whole genome shotgun sequence.
ACCESSION   DAOPUR010000354 DAOPUR010000000
VERSION     DAOPUR010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84155_length_1630_cov_3.067302"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKN90_06275"
     CDS             <1..408
                     /locus_tag="PKN90_06275"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNZ85915.1"
                     /translation="RRKADMVEWALYNAETLSQHKLVCTGTTGGLIQEAFENKSIAAD
                     ITRVNSGPMGGDAEIAAMVVKHQIDLAIFLIDDLNPQPHEADIQMLLRQCRVHNVPIA
                     CNRYSADLMITSTLWDNPTYQPTEPKYVVFERK"
     gene            481..1554
                     /gene="leuB"
                     /locus_tag="PKN90_06280"
     CDS             481..1554
                     /gene="leuB"
                     /locus_tag="PKN90_06280"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNZ85916.1"
                     /translation="MKLNIAVLPGDGIGPEIIKQGLDVTIAVCEKFGHELTYEYGLVG
                     ATAIDQVGNPYPDATHELCMKSNAVLFGAVGDPRFDNNPSAKVRPEQGLLAMRKKLGL
                     YGNIRPVTTFPSLIHKSPLRADLIDGADFVCIRELTGGMYFGRPQGRSEDGTVAYDTC
                     VYSRMEIERVLTLAYSYALKRRKKLTVVDKANVLATSRLWREVAKEMAQNYPDVETEF
                     MFVDNAAMRMIQWPKDFDVMVTENMFGDILTDEGSVITGSMGMLPSASIGEHTSVFEP
                     IHGSWPQAAGKDIANPLATILSAAMMFEYAFDLKEEAALIRKAVDASMDANVRTADIQ
                     VEGKEGYKCSEVGEWIKNWIENN"
BASE COUNT          530 a          246 c          351 g          503 t
ORIGIN      
        1 agaagaaagg cagatatggt agaatgggcg ttgtataatg ccgaaacttt atctcaacat
       61 aagttagtgt gtacaggtac tactggaggt ttaatacaag aagcttttga aaataaatca
      121 attgctgctg atattacacg agttaattca ggtccaatgg gtggtgatgc agagattgct
      181 gctatggtcg ttaaacacca aatagattta gctatatttt tgattgatga cttaaatcca
      241 caacctcacg aagctgatat tcaaatgctt ttgcgacaat gtagggtgca taatgtgcct
      301 attgcttgta atagatacag tgctgatttg atgataacta gtacgttgtg ggataatcct
      361 acttatcagc caactgaacc aaaatatgtt gtatttgaac ggaaatagta caacattagc
      421 aaaaaataac ccccaattat cttattaagt atacttttaa cataaacaat aactactaaa
      481 atgaaattaa acattgcagt tttgcctgga gatggcatag gaccagaaat tattaaacaa
      541 gggttagatg taaccattgc tgtttgtgaa aaatttggac acgaattaac ctatgagtat
      601 gggttagtgg gagctactgc cattgaccaa gttggtaatc cttatcctga tgctacacat
      661 gaattatgta tgaaatccaa tgctgtactt tttggagcag taggagatcc tcgttttgat
      721 aacaacccaa gtgctaaagt acgtcccgaa caaggcttat tagctatgcg taaaaaactt
      781 ggtttatatg gaaatattcg tcctgttaca acgtttccgt cgttgattca caaatctcca
      841 ttacgtgcgg atttaattga tggtgccgat tttgtatgta tcagagaatt aacaggtggt
      901 atgtattttg gtcgtcctca aggtagaagt gaagatggaa cagtagctta tgatacctgc
      961 gtttatagtc gtatggaaat tgaacgtgtg ttgacgttgg catatagcta tgcgttaaaa
     1021 cgcaggaaaa aattgactgt tgtagataaa gcaaatgtat tggcaacttc gcgtttgtgg
     1081 agagaagtag ctaaagaaat ggcacaaaat taccctgatg tagaaacaga atttatgttt
     1141 gtggataatg cggctatgcg tatgattcag tggcccaaag attttgatgt gatggtaacc
     1201 gaaaatatgt ttggtgatat tttaactgac gaaggttccg ttataactgg atctatggga
     1261 atgttgcctt cagcctctat aggcgaacat acttctgtat ttgaaccaat tcatggatcg
     1321 tggccccaag ctgctggaaa agacattgca aatccattag caactatttt atctgctgcc
     1381 atgatgtttg aatatgcttt tgacttaaaa gaagaagctg ctttaattcg caaagctgta
     1441 gacgcctcaa tggatgctaa tgtacgtacc gctgatattc aagtagaagg aaaagaaggg
     1501 tacaaatgca gtgaagtagg agagtggatt aaaaactgga ttgaaaataa ctaagttaca
     1561 tttatatttg ctagttaaac ataaaaaaac gaacatcgta atagaggttc gtttttttta
     1621 tgtttatatg
//
LOCUS       DAOPUR010000355         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_84217_length_1629_cov_3.512071, whole genome shotgun sequence.
ACCESSION   DAOPUR010000355 DAOPUR010000000
VERSION     DAOPUR010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84217_length_1629_cov_3.512071"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKN90_06285"
     CDS             complement(<1..440)
                     /locus_tag="PKN90_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNZ85917.1"
                     /translation="MKKILIACCASLLFSLSVFAQHDAQISQHLLSRTHYNPAAAGAS
                     LYGNITGIARQQYVGFTGAPQTGLLNFDMLLPNTSSGIGLSVLYDKYGPLLSYNPMLN
                     YAYHIKLGDNQHLSLGVAAGVFLRQYDASNNIYENIGDPLENYDG"
     gene            complement(458..>1629)
                     /locus_tag="PKN90_06290"
     CDS             complement(458..>1629)
                     /locus_tag="PKN90_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ85918.1"
                     /translation="YDLYSAQIDGKAVHKVQNLMKPINSSADDINLIVSEEKNEALLV
                     SSRNISTKDDIFRFEGIFTSMMVSGHAYNNVTKEVLPNTQVVLSGMGKTHTTETNKDG
                     YYYAFIEAGDLYKMFANAVGFLSDMAMIKAEKTTIGSFPVEQDFYLTPASMSISGRVY
                     DMETNEPFINEDVLLLENGTLAQQTKTDITGRYSFTNLTNGKEYQVKVNKPNFLSITS
                     KPFIYQEGDANNSSFDLASIPYGSDSFDPSGVGGVGGAGGMGNTSNVGGINGGRFAGR
                     EISINEIYYDFDNANLLPQSRFSLDKIILLLSSNPTLKLEFGSHTDTRGSAEYNLQLS
                     NARAKSVVDYLIASGISRNRLSWKGYGKTNPVVKNAVTEEEHRLNRRTTFKIIER"
BASE COUNT          469 a          316 c          299 g          545 t
ORIGIN      
        1 ccatcataat tttctaaagg gtctccaata ttttcgtaaa tattgtttga tgcatcatat
       61 tggcgtaaaa acacccctgc ggctactcct aacgaaagat gttggttgtc gcccaattta
      121 atatgatacg catagttaag cattggatta tacgataaca gcggtccata cttatcatac
      181 aatactgata accctatacc agagctggta tttgggagta acatgtcaaa gtttagcaag
      241 ccagtttgcg gtgctcctgt aaagcccaca tactgttgac gagcaatacc cgtaatgttg
      301 ccgtataacg aagctcctgc tgcagcaggg ttgtaatgcg ttctactcag caaatgttga
      361 ctgatttgtg cgtcgtgttg tgcaaaaacc gacaaagaaa acagaaggga tgcacagcaa
      421 gctataagta tttttttcat tgtgttcatg tttaaagtta tcgttcaata attttgaatg
      481 tggtacgtct gtttaatcga tgttcttctt ctgtaacagc atttttcact acaggattgg
      541 ttttaccata tcctttccac gaaagtctgt tgcgactaat gcccgaagca attaaatagt
      601 ccaccacaga ttttgcacgt gcattagata attgtaggtt gtattcagca ctaccacgtg
      661 tgtcagtatg cgaaccaaat tcaagtttta aggttgggtt agagcttaat aataaaataa
      721 ttttgtctaa tgaaaaacgt gattgtggaa gcaagttagc gttgtcgaaa tcgtaataaa
      781 tttcgtttat tgaaatttcg cgaccagcaa atcgtcctcc atttatacct ccaacattgc
      841 ttgtattacc cataccacca gcaccaccaa caccaccaac accacttgga tcaaacgaat
      901 ctgaaccgta aggaatggaa gctaaatcga acgagctatt gtttgcatct ccttcttggt
      961 aaataaaagg tttacttgta atagacaaga agtttggctt gttcactttt acttggtatt
     1021 cttttccatt ggttaaatta gtaaaggaat aacgtcctgt gatatctgtt tttgtttgct
     1081 gagccaaggt gccattttct aacagtagca catcttcgtt gataaatggc tcgttggttt
     1141 ccatatcata tacacgtccg ctaattgaca tactagctgg tgtaagatag aaatcttgtt
     1201 ctactgggaa tgaaccaata gtggtctttt ctgcctttat cattgccata tccgataaaa
     1261 atcctactgc attagcaaac attttgtata agtcgcctgc ttcgataaat gcataataat
     1321 atccatcttt atttgtttca gtggtatgtg ttttgcccat ccctgataat acaacttgtg
     1381 tatttgggag tacttctttt gttacattat tgtaagcatg ccccgatacc atcatgctgg
     1441 taaaaatacc ttcgaagcgg aatatatcat ctttggtaga aatattgcga ctacttacta
     1501 aaagagcttc atttttttct tctgacacaa ttaagttaat gtcatcggct gatgagttga
     1561 tgggtttcat caagttttgc actttatgta cggctttgcc atctatttgt gcactataca
     1621 aatcatatc
//
LOCUS       DAOPUR010000356         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_84385_length_1627_cov_4.059160, whole genome shotgun sequence.
ACCESSION   DAOPUR010000356 DAOPUR010000000
VERSION     DAOPUR010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84385_length_1627_cov_4.059160"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1627
                     /gene="uvrC"
                     /locus_tag="PKN90_06295"
     CDS             <1..>1627
                     /gene="uvrC"
                     /locus_tag="PKN90_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNZ85919.1"
                     /translation="FSKSLTSKKTAVLLKNISNIVYTVVDSEEEALLLENNLIKQHQP
                     RYNILLKDDKSFPYICIKNEPFPRIFKTRNVIKDGSEYYGPFTHMNTLKTLLDICKYQ
                     YTIRTCHLHLTKESILAHKFKVCLEYHIKNCKGPCVNKQTENDYLQQVNEVRSIIKGN
                     IKEVCSSILEEINVLANELRFEEAHILKQKLQILENYQSKWTVVNPSITHVDVFSIQE
                     NADESFVNYMNVVNGCIVQAYTVTYKKQLDETKETILGLAIIEMRERFNSKTNEIIVP
                     FVPDGKIKNAEFTVPLRGDKKKLLELSIKNALQYKVDRLKQAEKLNPEQRTTRIVKQL
                     QQDLHLNSLPLHIECFDNSNTQGTNPVSACVVFKSAKPSKKDYRHYHIKTVEGPDDFK
                     SMQEVVYRRYSRLLEENQPLPQLIIVDGGKGQLSSVYEILKKLDLDTKIAVIGIAKRL
                     EEIFFPNDPIPLYLDKKSETLKVIQHLRDEAHRFGITFHRKIRSKKQTVSSLDSIEGI
                     GEQTKMILLKTFKSVKRIKETPIEELEKIVGKAK"
BASE COUNT          665 a          306 c          269 g          387 t
ORIGIN      
        1 ttttctaaat cacttacctc aaaaaaaaca gccgttcttc taaaaaacat atccaatata
       61 gtttacacgg ttgttgacag tgaagaagaa gccttattgc tcgaaaacaa cttaatcaaa
      121 caacaccaac cacgctacaa catcttgcta aaagatgata agtcttttcc ttacatttgt
      181 atcaaaaacg aaccatttcc acgtattttc aaaacacgca atgtgataaa agacggatct
      241 gaatactatg gaccgtttac ccacatgaat accttgaaaa cgctgttaga tatttgcaaa
      301 taccaataca ctattcgtac atgccattta cacctaacca aagaatcaat actcgctcat
      361 aagtttaaag tctgtttaga gtaccatata aaaaattgca aaggtccctg tgtcaacaaa
      421 cagacagaaa atgactacct gcaacaagtt aatgaggtac gctcaataat aaaagggaat
      481 ataaaagaag tatgctcctc catactggaa gaaattaacg tactagcaaa tgaattacgg
      541 tttgaagaag cacacatact gaaacaaaag ttacaaattc ttgaaaacta ccaatcaaaa
      601 tggacagtgg taaatccaag cattacacac gttgatgtat ttagcataca agaaaatgcc
      661 gatgaaagct ttgtaaatta tatgaacgtt gtaaacggtt gtattgtaca agcatacaca
      721 gtaacataca aaaagcaact tgatgaaaca aaagaaacaa ttttaggact ggcaattatc
      781 gaaatgcgag aacgatttaa tagcaaaaca aacgaaatta ttgtcccatt tgtgccagat
      841 ggaaaaataa aaaatgctga attcaccgta cctttacgtg gcgataaaaa gaaactgctc
      901 gaattgtcga taaaaaatgc acttcaatat aaagttgaca gactaaaaca agcagaaaag
      961 ctaaacccag aacaacgaac aacaagaata gtaaaacaac tacaacaaga cctacatctg
     1021 aactctctcc cattgcatat agaatgcttt gacaattcaa atacacaagg aaccaatccc
     1081 gtatcagcat gtgttgtatt taaatcagcc aaaccatcga aaaaagatta ccgacactac
     1141 catataaaaa cagtggaagg acctgacgac tttaagagta tgcaagaagt ggtttacagg
     1201 cgatattcgc gattattgga agaaaaccag ccgttacccc aattaataat agtggatggc
     1261 ggaaaggggc agctaagcag cgtatatgag atattgaaaa aacttgactt ggatacaaaa
     1321 attgctgtaa ttggaattgc caaaagactt gaagaaatat ttttccccaa cgaccccatt
     1381 ccgctctatt tggacaagaa atccgaaaca ttaaaagtca tacaacattt gcgtgacgaa
     1441 gcccatcgtt ttggaatcac ctttcataga aaaatacgca gtaagaaaca aacagtttcg
     1501 agcttagaca gcatagaggg aattggcgag caaacaaaaa tgatattgct aaaaacattc
     1561 aaaagtgtaa aacgaataaa agaaacgcca attgaagagc tagaaaaaat tgtaggtaaa
     1621 gcgaaag
//
LOCUS       DAOPUR010000357         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_85536_length_1613_cov_3.924904, whole genome shotgun sequence.
ACCESSION   DAOPUR010000357 DAOPUR010000000
VERSION     DAOPUR010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85536_length_1613_cov_3.924904"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /gene="murF"
                     /locus_tag="PKN90_06300"
     CDS             <1..1180
                     /gene="murF"
                     /locus_tag="PKN90_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNZ85920.1"
                     /translation="NGNQYAMQALEKGAAYAIIDEPMQIHDARCIVVDNVLLTLQDLA
                     RFHRKKLTIPVLGITGSNGKTTTKELCAAVLRQKYKVHYTKGNLNNHIGVPLTILGIT
                     PDVEFAIIEMGANHPGDIQELVEIALPNYGIITNVGRAHLEGFGSFEGVIKTKKELHD
                     YILKANGKIFIDSDNSYLQEMALGTNQISYGTNYENYVSGRIKQVSPFVSFEWTKNDD
                     STYFIQTALIGEYNLSNLLAAVAVGVYFGVSALQCKDGIEAYVPENNRSQYMKTSKNE
                     LILDAYNANPTSMEAAIRNFKNITTSLPKMLILGDMFELGKEAQNEHQKIVDLLALLQ
                     FDKVCLVGSLFSQTKSNFTTLTNGDELVAYLQEKDFQGLSILIKGSRSMKMERVVEFL
                     "
     gene            1177..>1613
                     /locus_tag="PKN90_06305"
     CDS             1177..>1613
                     /locus_tag="PKN90_06305"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNZ85921.1"
                     /translation="MKIHMGDYCLDIYNYHRRNTDQVLIGTVWVGSKYPIRVQSMTNT
                     STMDTDASTAQCEELIDAGAELVRLTTQGVREAQNLYFIRKNLLAKGYVVPLVADVHF
                     NPKVAEEAARIVEKVRINPGNFVDSIKTFQQYEYTDEEYAKEIA"
BASE COUNT          565 a          226 c          335 g          487 t
ORIGIN      
        1 taatggaaat caatatgcta tgcaagcact agaaaaggga gcagcttatg ctattattga
       61 cgaaccaatg caaatacatg atgctcggtg tattgtagta gacaatgtac tacttacttt
      121 gcaggatttg gcacgttttc atcgaaaaaa actaacgata cctgtattag gaattacggg
      181 aagtaatggt aaaaccacca caaaagaatt gtgtgctgct gtattacgcc aaaaatacaa
      241 agtacactat acgaaaggga atttaaataa tcacattggt gtgcccttaa ctatattagg
      301 cattacgcct gatgtagagt tcgctattat tgagatggga gcaaatcacc ctggagatat
      361 tcaagagctt gtggaaatag cgttgcccaa ttatgggatt attaccaatg ttggcagggc
      421 tcatttagaa ggatttggtt cgtttgaagg tgttatcaaa acaaaaaaag aattacatga
      481 ttatattcta aaagcaaatg gtaaaatttt tattgattca gataattcgt atctgcaaga
      541 aatggcatta ggaacaaatc aaatttctta tggtactaac tatgagaatt atgtgagtgg
      601 aagaataaaa caggtttctc cttttgtttc gtttgaatgg acaaaaaatg atgattcaac
      661 ctattttatc caaacagcat taattggtga atataattta tctaatttat tagccgcagt
      721 tgctgtggga gtatattttg gtgtaagtgc attacagtgc aaggatggta ttgaagcata
      781 tgtgccagaa aataaccgtt cgcaatatat gaaaacgtcc aaaaatgaat tgattttgga
      841 tgcgtataat gcaaatccga caagcatgga agcagctatt cgtaatttca aaaatataac
      901 aacatcttta cccaaaatgt taatacttgg agatatgttt gaattaggta aagaggctca
      961 aaatgaacat caaaaaatcg tggatttatt agctctgttg caattcgata aggtctgttt
     1021 agtgggaagc ttattctctc aaacaaaatc aaattttacc acacttacta atggagatga
     1081 attggttgct tatttgcaag aaaaagattt tcaggggtta tcaatactaa taaaaggctc
     1141 aagaagtatg aaaatggaaa gagtcgttga atttttatga aaatacacat gggagattat
     1201 tgtcttgata tatataatta tcatcgtaga aatacagatc aggtgctcat tggtactgtt
     1261 tgggtaggtt caaagtatcc cattagagta cagtctatga cgaatacatc aacgatggat
     1321 actgatgcaa gtactgctca gtgtgaggaa ttaattgatg caggagcaga gttggtgcgt
     1381 ttaacaacgc aaggcgtaag agaggctcaa aacttatatt ttatacgtaa gaaccttttg
     1441 gcaaaaggct atgttgtgcc acttgtggca gacgttcatt ttaatcccaa agttgctgaa
     1501 gaagctgcaa gaatagtaga aaaagtgcgg attaatccag gtaattttgt ggatagtatt
     1561 aaaacttttc agcaatatga gtatactgat gaagagtatg ccaaagagat tgc
//
LOCUS       DAOPUR010000358         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_85743_length_1611_cov_2.670308, whole genome shotgun sequence.
ACCESSION   DAOPUR010000358 DAOPUR010000000
VERSION     DAOPUR010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85743_length_1611_cov_2.670308"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..>1611
                     /gene="atpD"
                     /locus_tag="PKN90_06310"
     CDS             150..>1611
                     /gene="atpD"
                     /locus_tag="PKN90_06310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNZ85922.1"
                     /translation="MSVKTARAYITQVIGPIVDIYFEEGGYALPEIHDALEIIKPSAK
                     NIIVECHQHIGERSVRTISMDSTDGLVRGMEVIPKNKPICMPIGNQIKGRLLNVIGDA
                     IDGIGELDRKGESPIHRDPPKYEDLTTTKEVLYTGIKVVDLLAPYSRGGKIGLFGGAG
                     VGKTVLIMELIHNIAKGYNGYSVFTGVGERTREGNDLLREMIESGVINYGEEFKKSME
                     EGDWDLSKIDHEALKTSMATLVFGQMNEPPGARASVALSGLTVAESFRDADASNAQGK
                     DILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLASEMGLLQERITSTKTGAITSV
                     QAIYVPADDLTDPAPATTFSHLDATTVLSRKIAELGIYPAVDPLDSTSRILEPNIVGE
                     LHYKTALRLVELLQRNKELQDIIAILGMEELSDEDKLIVHRSRRVQRFLSQPFHVAEQ
                     FTGIPGVFVPIEETIRGFNMIMDGEVDQYPESAFNLV"
BASE COUNT          536 a          289 c          318 g          468 t
ORIGIN      
        1 cttgctcaaa gtaagcacat tttaccttgg taaataaaat aaaaaaaaga atgttaaata
       61 atttgtttag ttcttttttt tacagtaact ttgggcgttc tttttacaat taacgagaaa
      121 cactaacctt tattctacaa acaaatacta tgtcagttaa gactgcaaga gcttatatta
      181 cacaggtaat tggacctatt gtggatatct attttgaaga aggtggatat gcccttcctg
      241 aaatccatga tgcattagaa ataatcaaac cttcagctaa aaacattatt gttgagtgcc
      301 accaacacat tggtgagcgt tctgtgcgta ccatatcaat ggattcgaca gatggtctag
      361 taagaggcat ggaagttatc cctaaaaaca agccgatctg tatgcctatt ggcaatcaaa
      421 taaaaggacg attacttaac gtaattggcg atgccataga tggaataggc gaacttgata
      481 gaaaaggaga atcacctatc cacagagacc ctcccaaata cgaagattta acgacaacaa
      541 aagaagtact atatacaggt attaaagttg tagatttact tgctccctac tcacgtgggg
      601 gtaagattgg actctttgga ggtgctggtg taggtaaaac agtacttatt atggaattaa
      661 tccataatat tgcaaaagga tacaatggtt attctgtttt tactggcgta ggcgaacgta
      721 cacgcgaagg aaatgattta ttgcgagaaa tgattgaatc tggcgtaatc aattatggag
      781 aagaattcaa aaaaagcatg gaagaaggcg attgggattt atccaaaatt gaccatgaag
      841 ccttaaaaac ctcaatggct acgctcgtat ttggacaaat gaatgaacct cctggagcaa
      901 gagcctctgt ggcactttct ggattaacgg tagctgaatc atttcgtgat gccgatgcaa
      961 gcaatgctca aggaaaagat attttatttt ttatagacaa tatcttccgt tttacacaag
     1021 caggttcaga ggtttctgca ctactaggta gaatgccgtc tgctgtagga tatcaaccta
     1081 ccttagcttc agaaatggga ttgctacaag agcgtattac ttcgactaag acaggtgcaa
     1141 tcacttctgt tcaagccatt tacgttcctg cagatgactt aactgaccca gccccggcaa
     1201 ccacattctc tcacttagat gcaacaactg ttttaagtag aaaaattgca gagctaggaa
     1261 tctaccccgc agtagaccct cttgattcaa catcacgtat tttggaacca aatattgtag
     1321 gcgaattaca ttacaaaact gcattaaggc tagttgagct attacaacgc aacaaagagt
     1381 tacaagacat tattgctatt ttaggaatgg aagaattatc tgatgaagat aaattaattg
     1441 tacatcgttc aagaagagta caacgatttt tatctcaacc atttcacgtt gctgaacaat
     1501 ttacagggat acccggtgtt tttgtaccaa tagaagaaac tattcgtggg tttaatatga
     1561 ttatggatgg agaagttgac caataccctg agtctgcttt caacttagta g
//
LOCUS       DAOPUR010000359         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_86414_length_1603_cov_2.751292, whole genome shotgun sequence.
ACCESSION   DAOPUR010000359 DAOPUR010000000
VERSION     DAOPUR010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86414_length_1603_cov_2.751292"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..>1603
                     /locus_tag="PKN90_06315"
     CDS             315..>1603
                     /locus_tag="PKN90_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85923.1"
                     /translation="MKNWISQPFVPHIVAVVVFFLLTFFYLTPAFQGNIIFGHDTLSY
                     VASAKEAHTYNEQNDDQALWTNSMFGGMPTYQISMTQPQSILTYIDAVLRVLPGPSYR
                     LFLYLVGFYVLLLAFRVNPWLSIGGAIAFALGSYNLIIIIAGHNTKAVAIAYMAPIIG
                     SVYLAFREKRLLGTVLLTLFLGLSLFANHIQITYYTLFIVLCFGVSEFIFSVKEKKLV
                     PFFTTVGILLVGVVIAVGLNATRLLTTAEYAAYTMRGASNGLSLDNGQEQKGLSKDYI
                     TQWSYGIDETLTLFIPNVKGGSSVGKLTEDSQTAMKFAELSGYKPLGRNLTKEEQSRI
                     DSILKRNPDNFTPKNLAELMCMYELPLYWGTQPFTSGPVYIGAIVIFLFFAGLYLLPA
                     RDKWWMLAATLLGIMLAWGKNFMPLTDFFIDYVPMYN"
BASE COUNT          447 a          297 c          311 g          548 t
ORIGIN      
        1 caactataac tatcttataa ggattttata atccgctgaa catacatttt tttacactaa
       61 cacctcctcg ttttcaacga ggagtttttg gtttgttgtt tgtaacaaca agttgatgca
      121 aatccactca tcaaaattaa caaactaaat tccaaacaaa tacacatact tactaattcc
      181 caacgcagca attagctaaa aaacatcctt ttctgcatat tatttcattt tttcaacaaa
      241 aaatacttac tttgtggtgt cttacaaaaa gcatagtatt tgctgctaat aacttgaccc
      301 ttatacccaa aaaaatgaaa aattggattt ctcaaccatt tgttccacat atagtggctg
      361 ttgtagtatt ttttctactt actttttttt atttaactcc cgcatttcaa ggaaatatca
      421 tttttggtca cgatacgctt agctatgtag cttcagctaa agaagcacat acctacaacg
      481 agcaaaacga cgaccaagcc ctatggacaa attccatgtt tggcggtatg cctacttatc
      541 aaatttcgat gacgcaacct caaagtatat taacctatat cgatgctgta ctcagggtgt
      601 tgccaggtcc tagttatcgc ttgtttttgt atttagttgg tttttatgtg ttgttgttgg
      661 cgtttcgtgt caatccatgg ttgagcatag gaggtgctat tgcgtttgct ttggggtcgt
      721 acaatttaat cattattatt gcaggtcata ataccaaagc ggttgccatt gcctatatgg
      781 cacctattat tggcagtgtg tatcttgctt ttagagagaa acggctgctc ggtaccgtat
      841 tgctgacctt gtttttaggc ttatcgcttt ttgctaacca catacaaatt acctattata
      901 ccctgtttat tgtgttgtgt tttggggtgt cggaatttat ttttagtgta aaagagaaaa
      961 aacttgttcc tttttttact actgttggca tcttgttggt gggtgtcgtt attgcggttg
     1021 gcttaaatgc cacacgtttg cttacaacag ccgaatatgc tgcttatacc atgcgtggag
     1081 cttctaacgg tttatcgtta gataatggac aggagcaaaa gggattgagc aaagactata
     1141 ttacccaatg gagctatggc atagacgaaa cgcttacgct gtttatccca aatgtgaaag
     1201 gtggctcatc tgtgggtaag ttaacagaag attcccaaac agctatgaaa tttgcagagt
     1261 tgtcggggta taaacctttg ggtcgaaact taaccaagga agagcaaagt cgtatagata
     1321 gcattttgaa acgtaatcca gataatttta ctcccaaaaa cttagcagag ttgatgtgta
     1381 tgtacgagtt acccctgtat tggggaaccc aaccttttac atcgggtcca gtgtatatcg
     1441 gagccattgt aattttctta ttttttgcag ggttgtacct attgcctgca cgcgacaagt
     1501 ggtggatgtt agctgctacc ttactaggca ttatgttggc gtggggaaag aattttatgc
     1561 cgctaactga tttctttatt gattatgtgc caatgtacaa taa
//
LOCUS       DAOPUR010000360         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_86424_length_1603_cov_2.574289, whole genome shotgun sequence.
ACCESSION   DAOPUR010000360 DAOPUR010000000
VERSION     DAOPUR010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86424_length_1603_cov_2.574289"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKN90_06320"
     CDS             complement(<1..590)
                     /locus_tag="PKN90_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ85924.1"
                     /translation="MQFKRVLTCIIGLSIMLIFSQCSTKNNTWLSRNFQSFSTRYNVY
                     FNANESYKKGIEKIALGHKDSYTEILPIFPISVHTNASFATNEMAISIEKSEKAIKNH
                     SIKRKPKRNPSKLQDPSYQAFLNQSEYNPMVEQSWLLIGKSQFHQADFMAANATFLYI
                     IRNYIHNQELVTAATIWLARSYGEVGWFYEAEDLMN"
     gene            823..>1603
                     /locus_tag="PKN90_06325"
     CDS             823..>1603
                     /locus_tag="PKN90_06325"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNZ85925.1"
                     /translation="MKSKVTVVGAGNVGATCANVLAINEVASEVVLLDVKEGVAEGKA
                     MDIMQTAQLMGFDTVVKGCTNDYAQTANSDVVVITSGVPRKPGMTREDLIGINAGIVK
                     SVSQSILEYSPNAILIIVSNPMDTMACLAMKILGLPSNRIIGMGGALDSSRFTYYLSQ
                     ALQCNANDVQGMVIGGHGDTTMVPLTSLATYKGVPVSKLLSKEKMEEVAKATMVGGTT
                     LTGLLGTSAWMAPGAASAVVVEAILRDQKKIIPCSVVLNGEY"
BASE COUNT          506 a          241 c          346 g          510 t
ORIGIN      
        1 ttattcatca ggtcttctgc ctcataaaac caaccgactt caccatagct acgagccaac
       61 catattgttg ctgctgtaac caattcttgg ttatgaatat aattacggat aatatacaaa
      121 aaagtggcat tagctgccat aaaatcagct tgatggaatt gtgattttcc aattaaaagc
      181 caagattgtt ccaccattgg attgtattct gattgattca gaaaagcctg ataggatggg
      241 tcttgtaatt tggatggatt gcgttttggc ttgcgtttta tggagtggtt ttttatggct
      301 ttttcgcttt tttctatgct gatagccatt tcgttggtag caaaagaggc atttgtatgc
      361 acgctgattg gaaaaatggg caatatttcg gtgtaggaat ctttgtgtcc taaagcaatt
      421 ttttctattc cctttttata gctttcattg gcattgaaat aaacattata gcgtgtggaa
      481 aaagactgaa aattacgaga caaccatgtg ttgttttttg ttgaacactg gctaaaaata
      541 agcataatcg acaacccgat tatacaggta agcactcgtt taaattgcat agtatatccc
      601 attttcggta caaaagtaca caaattatgc tagtatgcta tgaaaaatag cttcgttttt
      661 tgtaaattta agtgttcctg tcttttattg ttgaaaacag ggtgtaaaaa atgttatttt
      721 tttcttaagg aggtgctgat ttattctctt ctttctttga tatgaaagaa aaaattgtaa
      781 ttttgtgggc agtaaaatca acacttttta aaaatacaaa taatgaagtc gaaagtaacc
      841 gttgtaggag caggaaatgt aggggcaacc tgtgctaatg tattagcaat taatgaagta
      901 gcaagcgaag tcgttttgct agatgtaaaa gaaggtgtgg ctgaagggaa agccatggat
      961 attatgcaaa cagcccaatt aatgggattt gacacggtag taaaaggttg taccaacgat
     1021 tatgctcaaa ctgccaattc agatgttgtg gtaattacgt cgggagtacc acgtaaacca
     1081 ggtatgacaa gagaagattt aataggtatt aatgccggta ttgttaaaag tgtatcacaa
     1141 agcatcttag aatattctcc caatgcaata ttaattatcg tttccaaccc aatggatacg
     1201 atggcatgtt tggctatgaa aatattagga ttaccttcaa acagaattat tggtatggga
     1261 ggagcattag atagttcacg ttttacgtat tatttgagcc aagcattgca atgtaatgcc
     1321 aacgatgtgc aagggatggt aattggagga catggcgata ctacaatggt gccacttacc
     1381 agtttagcta cgtataaagg cgttccagta agcaaattgt taagcaaaga aaaaatggaa
     1441 gaagtggcaa aagctaccat ggtaggaggt actacgttaa ctggactttt aggaacatca
     1501 gcatggatgg ctccaggtgc agcatcggct gttgtggtgg aagctatatt acgtgaccaa
     1561 aagaaaatta ttccttgttc ggttgtttta aatggtgaat acg
//
LOCUS       DAOPUR010000361         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_86575_length_1601_cov_3.518111, whole genome shotgun sequence.
ACCESSION   DAOPUR010000361 DAOPUR010000000
VERSION     DAOPUR010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86575_length_1601_cov_3.518111"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKN90_06330"
     CDS             complement(<1..980)
                     /locus_tag="PKN90_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNZ85926.1"
                     /translation="MGNYLEIICKNNQKKALIPFGRSLAEVFEDLKIELPYQVVAARV
                     NNKIESLNYCLYRNKTIEFVDLSCESGMRVYVRSLVFVLFKAIVDLIPNGRLRVEHPV
                     SKGYYCEITANGEKISFEQLAAIKIRMKEIIEQNIRFVSEDKPTSEVIEVFAKHNRTD
                     NVDLLQTYKNYYTEYHSLDGLPDFYSGVVLPSTGYLSLFDIHPYYKGFLIQIPKRSNP
                     IELEEIVVQNKLLEVFADHYKWCRIMGINNIADFNNIEKGKLRDFIKVTEALHEKQIS
                     KMADTIAERSEKTKIVLVSGPSSSGKTTFTKRLSVQLMASGIKPISLSLD"
     gene            1079..>1601
                     /locus_tag="PKN90_06335"
     CDS             1079..>1601
                     /locus_tag="PKN90_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNZ85927.1"
                     /translation="MEYTFFDLLVLIGSLGLFLYGMKLMSEGLQRVAGDSLRSVLAAM
                     TKNRIMGVITGVLVTAIIQSSSATTVMVVSFVNAGLLTLFQSITVIMGANIGTTVTAW
                     IISLFGFKVSIAAFAVPLIAFAFPLVFSSNNKRKSLGELILGFALLFMGLDMLKNAVP
                     DLRSNPEILAFLTQ"
BASE COUNT          466 a          279 c          272 g          584 t
ORIGIN      
        1 ttgtctaatg ataaagaaat gggtttaata cccgacgcca taagttgcac actcaatcgt
       61 ttggtaaagg tagttttacc agaagaagaa ggacctgaaa ccaatacaat ttttgttttt
      121 tcactccgtt cggcgatagt atctgccatt tttgaaattt gcttctcgtg taaagcttca
      181 gttactttaa taaaatcacg tagcttgcct ttttctatgt tattgaaatc agctatatta
      241 ttgatgccca ttattctgca ccatttataa tgatcagcaa aaacttctag taatttattt
      301 tgtaccacaa tctcttcaag ttctattggg ttactacgtt tgggtatttg tatcaaaaaa
      361 cctttataat acggatgtat atcaaataga gataaataac cagtgctggg taatactacg
      421 ccactgtaaa aatcgggtaa tccatcaagc gaatggtact ctgtgtaata gtttttatat
      481 gtttgcaaca aatcaacatt atctgttctg ttgtgttttg caaaaacttc aattacctcc
      541 gatgttggtt tgtcttctga tacaaatcgg atgttttgct caatgatttc tttcatacgt
      601 attttaatag cagctaattg ttcaaaagag attttttctc cattagccgt aatttcgcaa
      661 taatatccct tcgacactgg atgttctacc cgaagcctcc cattgggaat taagtctaca
      721 atggctttaa acaatacaaa caccaacgaa cgtacatata cacgcatgcc cgattcgcaa
      781 ctcaaatcta caaattcgat agttttattg cgataaaggc aataatttaa gctttcaatt
      841 ttattattta ctcgagcggc taccacttga taaggtagtt caatttttaa atcttcaaat
      901 acttctgcta agcttctacc aaaagggatt aaagctttct tttggttgtt cttacagata
      961 atctcaaggt agtttcccat aactgtatgc tatatttatt taacttttat ttaaaaaatc
     1021 tttgtaaatg tataaaaaat attatttttg taccttactt ttttaaataa aatccgttat
     1081 ggaatacaca ttttttgatt tattagtatt aattggctca ttgggtttgt ttttgtatgg
     1141 aatgaagctg atgagtgaag gtttgcaacg agtagcaggc gatagcttac gttcagtatt
     1201 ggcagcaatg accaaaaacc gtatcatggg ggttattaca ggcgttttag ttacggctat
     1261 catccagtcg tcttctgcaa ccacagtgat ggttgtaagt tttgttaacg caggattatt
     1321 aacgcttttc caatcaatca ccgttatcat gggagccaat ataggtacaa ctgttaccgc
     1381 ttggataatt tctctgtttg gttttaaggt tagtattgct gcatttgccg ttccactgat
     1441 tgcttttgca tttccattgg tattttcttc taataacaaa agaaaatcct taggagagct
     1501 gatcttgggt tttgcccttt tgtttatggg cttggatatg ttgaaaaatg ccgttcctga
     1561 tttacgaagt aatccagaaa ttttggcgtt cttaacccaa t
//
LOCUS       DAOPUR010000362         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_87183_length_1594_cov_3.634828, whole genome shotgun sequence.
ACCESSION   DAOPUR010000362 DAOPUR010000000
VERSION     DAOPUR010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87183_length_1594_cov_3.634828"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..>1594)
                     /locus_tag="PKN90_06340"
     CDS             complement(207..>1594)
                     /locus_tag="PKN90_06340"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNZ85928.1"
                     /translation="QNVDEKEQCWIIYNNITNLYIPHPDRLRNWLNTPKANGYEALHV
                     TVMGPDGQWIEVQIRSARMDDIAEKGLAAHWKYKQQFYGDSELDKWLNTINDILKNPS
                     PNAMDFLDTFKLTLYATEIFVFTPKGEIKTLPSGSTALDFAFEIHSDIGMRCIGAKVN
                     NKLVPLSYELQSGDQIEILTSKQQTPQPEWLSYVTTARAKQKLNLLFKNEQKIVKAEG
                     ETILRKALTEAGFVIKKDISNDIIKKLLHHFEINSFEELTFQIGKGSISLANIQDIVK
                     QENKKTTVRSWYPRFFFGKDTDKENTATASARIKKNGTYQLTDEMLQKEHTIAPCCKP
                     IPGDQIIGFINDKKEIIVHKRDCKEALKLKSQKGSSIITAEWANTQKKYYATTIEVQG
                     VDRKRILLDILTEISDKLDANIEKLCVETNDGIFSAKMNVYVSHVDTINQIYKQLKII
                     NGVQRVARIEE"
BASE COUNT          411 a          270 c          304 g          609 t
ORIGIN      
        1 agagtctatt tcagttagtt gctcttctat tcctttcaga aaagcttctt tgtaatgttc
       61 ttcttttatc tcgttaaaag gtacagtttg gtaaggagtt gtataacgag cggttaaaaa
      121 agggtttgcg taactagtca tagaaaaaag tattagataa cataaaatat gagatatata
      181 ggcattcata tgtctgtata aaggtgttat tcttcgatac gtgctacacg ttgtacacca
      241 ttaataattt tcagttgctt gtatatttgg ttaattgtat ctacgtggct tacatacacg
      301 ttcattttgg cagaaaaaat accatcgttt gtttctacgc ataatttttc aatattagca
      361 tctagtttat cagatatttc agttaaaata tctaacaaaa tacgttttct atctactcct
      421 tgtacttcga tagtggtagc ataatatttt ttttgtgtat tagcccattc ggcagtaatg
      481 atgctcgacc ctttttgcga tttgagtttt aacgcttctt tgcagtctcg tttgtgtacg
      541 attatttctt ttttgtcatt tataaaacca ataatttgat cgcctggtat gggtttgcaa
      601 cacggagcaa tagtgtgttc tttttgcagc atttcatctg taagctggta ggtgccattc
      661 tttttaatac gagccgaagc tgttgctgta ttctctttat ccgtatcttt gccaaagaag
      721 aagcgaggat accacgaacg cacggtggtt tttttattct cttgttttac aatatcttga
      781 atattggcaa gagaaatact gcctttgccg atttgaaaag taagttcttc aaaactgttg
      841 atttcaaaat ggtgcaacaa ttttttgatt atatcgttgc taatatcttt ttttattaca
      901 aatccagctt cagtaagtgc tttgcgtagg atagtttccc cttctgcttt tactattttt
      961 tgttcgtttt taaaaagcaa attaagtttc tgttttgctc gagctgttgt aacatagcta
     1021 agccattctg gttgaggtgt ttgttgtttt gatgtaagta tttctatttg gtcgccactt
     1081 tgtagttcgt agctcaatgg tactagtttg ttgtttactt tcgctccaat acatcgcata
     1141 ccaatatcgg agtgaatttc gaaggcaaaa tccaaggcag tggagcctga gggtaaggtt
     1201 tttatttcgc ctttgggtgt aaatacaaat atttcggtag cgtacagcgt aagcttgaag
     1261 gtatcaagaa agtccatggc attggggctt ggatttttca agatatcgtt aatcgtgttt
     1321 agccatttat ctaattccga atcgccataa aactgttgtt tgtatttcca gtgtgcagcc
     1381 aatccttttt cggcaatatc gtccatgcgg gcacttctaa tttgcacctc tatccattgc
     1441 ccatctggac ccattacggt tacgtgtaag gcttcataac cattggcttt gggtgtgttg
     1501 agccaatttc tcaacctatc gggatgagga atatagaggt tggtaatatt gttgtaaata
     1561 atccaacatt gttctttttc atctacattc tgat
//
LOCUS       DAOPUR010000363         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_87269_length_1593_cov_3.485046, whole genome shotgun sequence.
ACCESSION   DAOPUR010000363 DAOPUR010000000
VERSION     DAOPUR010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87269_length_1593_cov_3.485046"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PKN90_06345"
     CDS             <1..1134
                     /locus_tag="PKN90_06345"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNZ85929.1"
                     /translation="AIMAEYKIGGIPVVDDNKILVGIVTNRDLRFERNLDRFIDEVMT
                     KENLVTTKQSTDLESAAQILQQYKIEKLPVIDNNGKLIGLVTYKDITKAKDKPMACKD
                     TKGRLRVAAGVGVTDDTLDRMQALVDAGVDAIVIDTAHGHSISVIEKLKEAKKRFPQI
                     DIVVGNIATGEAAKTLVAAGADAVKVGIGPGSICTTRVVAGVGVPQLTAVYDVAKALK
                     GTGIPLIADGGLRYSGDVVKALAAGGFSVMIGSLVAGTDESPGETIIFNGRKFKTYRG
                     MGSLEAMEKGSKDRYFQSGEFDVKKLVPEGIAARVPYKGSLYEVIFQMVGGLRAGMGY
                     CGARDIEELHNAKFTRITAAGVKENHPHDVTITHEAPNYTRGE"
     gene            1151..>1593
                     /locus_tag="PKN90_06350"
     CDS             1151..>1593
                     /locus_tag="PKN90_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85930.1"
                     /translation="MKKWIVFIFVSLSVSYTLAQKNTIVTIDQKPISIEEFEYYYNKN
                     NTNDLQQKMPVEEYMQLFVNFKLKVHQAYMLQMDTAQTFKQELEGYRNQLIKPYLTDN
                     EKIDDLVKEAYQHLLEDVDVSHILIRLNANPTPADTIAAYTKAMTA"
BASE COUNT          584 a          276 c          316 g          417 t
ORIGIN      
        1 gctattatgg cggaatacaa gattggagga atacccgtag tagatgataa caaaattcta
       61 gtaggcattg ttacaaaccg cgatttacgt tttgaacgta atcttgaccg ttttatcgac
      121 gaagtaatga caaaagaaaa tttggtaact accaaacaat ctaccgatct tgaatcagca
      181 gctcaaatac tacagcaata taaaattgaa aaattacctg taattgataa taatggcaaa
      241 cttattggat tggttacata caaagacata actaaagcca aagacaaacc tatggcgtgc
      301 aaagatacca aaggacgatt gcgtgttgct gcaggcgttg gagtaactga cgatacttta
      361 gatagaatgc aagccctagt agatgcaggg gtagatgcta ttgttattga tactgctcac
      421 ggacactcaa tttctgtaat tgaaaaacta aaagaagcaa aaaaacgttt tccccaaatt
      481 gatattgtag taggaaatat tgcaacagga gaagctgcta aaacattagt ggctgctgga
      541 gccgacgctg taaaagtagg catcggtcca ggttccatct gcacaacaag agtggtagct
      601 ggcgtaggtg tacctcaatt aacagctgta tatgatgtag ccaaagccct aaaaggaaca
      661 ggtatacctt taattgcaga cggaggactt cgctattcag gagacgttgt aaaagcatta
      721 gccgcaggag gtttttctgt aatgataggc tccttggtcg caggaacaga cgaaagtcca
      781 ggtgaaacca tcatatttaa cggcagaaaa tttaaaacat acagaggaat gggttcttta
      841 gaagcaatgg aaaaaggatc taaagaccgc tacttccaaa gtggtgagtt tgatgtaaaa
      901 aaattggtac cagaaggcat tgccgcacga gttccttata aaggctcttt atacgaggta
      961 atttttcaga tggttggtgg attacgtgct ggaatgggct attgtggagc tcgcgatata
     1021 gaagaattgc acaatgctaa atttacacgt attactgctg ctggagtaaa agaaaatcac
     1081 cctcacgatg taactatcac tcacgaagca cctaattaca ctagaggaga ataaactaaa
     1141 ttttgattgt atgaaaaaat ggattgtgtt tatatttgtc agtttaagtg ttagctacac
     1201 cctagctcaa aaaaacacta tagtaactat tgatcaaaaa cctatttcaa tagaagaatt
     1261 tgaatattac tataataaaa ataatacaaa tgatttgcag caaaaaatgc ctgttgaaga
     1321 atatatgcaa ttgtttgtaa actttaaact gaaagtacat caagcttata tgttacaaat
     1381 ggatacagcc caaacattta aacaagaact agaaggatac cgtaatcaac taataaaacc
     1441 atacttaaca gataacgaaa aaattgatga tctcgtaaaa gaagcttacc aacatttact
     1501 cgaagatgtt gatgttagcc acattttaat acgactaaat gcaaacccaa cccctgcaga
     1561 caccattgct gcatacacaa aagcaatgac cgc
//
LOCUS       DAOPUR010000364         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_88490_length_1579_cov_3.176509, whole genome shotgun sequence.
ACCESSION   DAOPUR010000364 DAOPUR010000000
VERSION     DAOPUR010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88490_length_1579_cov_3.176509"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PKN90_06355"
     CDS             <1..1203
                     /locus_tag="PKN90_06355"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNZ85931.1"
                     /translation="VGLRAKLLVGLLDANLGFNELELQASQEEWRIIGNASARFSAPT
                     VTVLVDNDGKIEEVAIPDDVLNSLRDYRPNLGLGFDLGAVYKPIENLSLSVAIRDLGF
                     ISWKNGYQQSASIDFAFEGIEFDITQTDSDYADSLLTVLQDSYTVEEMKTGYTSMLKS
                     KIYLGAEYAFLNNKMSVGLLSKTTVENSQAFGELTASLNMRPLSWFGTSFSYSLFNGN
                     FSSMGIGVNLRIPPFSFYIAGDYFPLYFSSEMIPYKMRGMNMQTGMVMTFGCKKKKIK
                     EVQEEDKSTKMLRLQQEMMLLQQEMDKESKEQLNDSSKTNNSDVLYQPIQRMQDPTGV
                     LKAIEDGEITLEEATKEGVMEVKVIPENVQQQHSEGESGIPSAMEATPTIENMVPNTI
                     TNTEPTNE"
     gene            1238..1420
                     /locus_tag="PKN90_06360"
     CDS             1238..1420
                     /locus_tag="PKN90_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85932.1"
                     /translation="MKQEINQGATSGAVYGLGLIGAAIYFISQATTFWLGVLGFLKAL
                     AWPAFMVYEALKFLGV"
BASE COUNT          546 a          234 c          321 g          478 t
ORIGIN      
        1 gtcggattac gtgccaaatt gttggtaggg ttacttgacg ctaacttagg ttttaatgaa
       61 ttagaattac aagcatcaca agaagaatgg cgaattatag gaaacgcttc agctcgtttt
      121 tcggcaccta cagttactgt attagtcgat aatgatggta agatagagga ggtggcaata
      181 ccggatgatg ttttgaattc gttgagagat tatcgtccaa acctagggct tggttttgat
      241 cttggggcag tatacaaacc aatagaaaac ctaagtttat cggttgcaat tcgtgattta
      301 ggctttattt cgtggaaaaa tggttaccaa caatctgctt cgattgattt tgcttttgaa
      361 ggaatagaat ttgatattac gcaaacggat tcagattatg cagattcttt gcttactgtg
      421 ttgcaagatt catatacggt agaggagatg aaaacaggat acacatccat gcttaaatca
      481 aaaatttacc ttggagctga atatgctttt cttaacaaca aaatgagtgt tggcttatta
      541 tccaaaacca cagttgaaaa ttcacaagct tttggtgaat taacagcttc gttaaatatg
      601 agaccattaa gttggtttgg aacttcattt tcatactctt tatttaatgg caacttcagt
      661 tctatgggaa taggggttaa cttacgtatt cctccattta gtttttatat agcaggagat
      721 tatttcccat tatatttcag tagcgagatg ataccatata aaatgagagg aatgaatatg
      781 caaactggta tggtaatgac ttttggatgc aaaaagaaaa aaataaaaga agtgcaagaa
      841 gaagataaat cgactaaaat gttgcgtttg caacaagaga tgatgctttt acaacaagaa
      901 atggacaaag aatcaaaaga acagctaaac gattcttcta aaacgaataa ttctgatgta
      961 ctatatcaac ccatacaacg tatgcaagac cctactgggg tgctaaaagc aattgaggat
     1021 ggcgaaatta cacttgaaga ggcaacaaaa gagggcgtaa tggaggtgaa agttatacct
     1081 gagaatgtac agcaacaaca tagtgaagga gaaagtggta ttcctagtgc tatggaagca
     1141 actccaacta tcgagaatat ggtgccaaat acaattacta atacagaacc aacaaatgaa
     1201 taaacataga attaattcaa tttaataagc attttttatg aaacaagaaa tcaatcaggg
     1261 agctacttct ggagcagttt atggtttagg attaattggt gctgctattt attttatttc
     1321 gcaggcgacc actttttggt tgggcgtttt ggggttttta aaagcactgg cttggccagc
     1381 atttatggtt tatgaggcat taaagtttct tggagtataa ataagtatac aagtaccaca
     1441 aatctataca caatcaaaaa atgttgcaaa cattaattat tcgtatgctg tgaatgcctt
     1501 accaataaca actacacttg ttgatgtggt agtaacaaca ggtacagcag gttcatatac
     1561 aattagttta gataatata
//
LOCUS       DAOPUR010000365         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_89238_length_1570_cov_4.359076, whole genome shotgun sequence.
ACCESSION   DAOPUR010000365 DAOPUR010000000
VERSION     DAOPUR010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89238_length_1570_cov_4.359076"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKN90_06365"
     CDS             <1..614
                     /locus_tag="PKN90_06365"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNZ85933.1"
                     /translation="LKQAISMNVQHISAYHLTYEPGTLFYKQKTKKIITEKDEEESIQ
                     LYEILIQSLTEAGYNQYEISNFSLPDYESKHNSSYWKNTPYIGLGASAHSYNGASRQW
                     NIASVITYCKQIEENKSFFEIEFLTEKEKYNDFIITSLRTSKGVDLQKLTTLFGEKSR
                     SYFIQQCQPYVHTNMLCEKDNFVHLTQKGIFVSDSILETLIDV"
     gene            625..996
                     /locus_tag="PKN90_06370"
     CDS             625..996
                     /locus_tag="PKN90_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNZ85934.1"
                     /translation="MLQFVRSYWKTIVTAIFIAYLSLFKPNSTIQLPPIPFVDKWIHI
                     VLYTTLTAVALLDTKKRKNNTSSFLLVLLISILYGGLMEILQQFFPPRTSSWYDFLAN
                     ILGCLLAFGSITWFVKKKRNG"
     gene            989..>1570
                     /locus_tag="PKN90_06375"
     CDS             989..>1570
                     /locus_tag="PKN90_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNZ85935.1"
                     /translation="MDNKLLYQIAITQIKGLGSINSKNLLAYVGDIEEIFHQKSHILQ
                     KIPGIGILLSKSILEQKDAALEKATKEIQFITKHGITPLFYTDKNYPERLKECPDAPI
                     LLYYKGNTNLNTQKTLAIVGTRNATEYGKTMCTQLIKELANNHPELCIISGLAYGIDI
                     CAHKEALHMHLPTVAVLGHGLDRIYPAQHATIAK"
BASE COUNT          570 a          294 c          239 g          467 t
ORIGIN      
        1 cattaaaaca agccatttcg atgaatgtgc aacatatttc ggcgtatcat ttaacttacg
       61 aacctggcac actcttttac aagcaaaaaa cgaaaaagat aatcaccgaa aaagacgaag
      121 aagaaagtat tcaattgtat gaaatcctta tacagtcgtt aaccgaagct ggttataatc
      181 aatacgaaat ttcgaatttt tcactccctg actacgaatc taaacacaat tcatcgtact
      241 ggaaaaatac accctatatt ggattaggtg cttcggctca ttcctacaac ggagcatctc
      301 ggcaatggaa catcgcatct gtaattacct attgcaaaca gattgaagaa aataaatcat
      361 ttttcgaaat tgaattttta acagaaaaag aaaaatacaa cgattttatc atcacctccc
      421 ttcggactag caaaggggtt gatttgcaaa aacttactac gctatttgga gaaaaatcaa
      481 gaagttattt tatccaacaa tgccaaccat acgtacatac caacatgctt tgtgaaaaag
      541 ataattttgt gcatcttact caaaaaggta tttttgtatc cgattctatt ttagaaacat
      601 taattgatgt gtaacaactc tgcaatgcta caatttgtac gttcatattg gaaaaccatt
      661 gtaacagcaa tcttcattgc gtatttgtcg ctattcaaac caaatagcac cattcaacta
      721 cctcctattc cttttgtaga taaatggata cacattgttc tgtatactac gctaacagca
      781 gtagccctac ttgacacaaa aaaaagaaaa aataatacgt catcatttct tcttgtattg
      841 ttaattagca ttctatatgg aggtttgatg gaaatattgc aacagttttt ccctcctcgc
      901 accagcagtt ggtacgattt tttggcaaat atacttggct gtttacttgc ttttggtagt
      961 attacatggt ttgttaagaa aaaaagaaat ggataacaaa cttctatacc aaatagccat
     1021 tactcaaatt aaaggattag gcagtataaa tagtaaaaat ttactcgcct atgtgggcga
     1081 tattgaagaa atatttcatc aaaaatcgca tattttacaa aaaataccgg gtattgggat
     1141 tctgttaagc aaaagcattc ttgaacaaaa agatgctgca ctcgaaaaag ccaccaaaga
     1201 aattcaattt atcactaaac atggcattac acctcttttc tataccgata agaattatcc
     1261 agaacgatta aaagaatgtc cagatgctcc aattctgttg tattataaag gaaatacaaa
     1321 tttaaataca caaaaaacct tagccatagt aggcacacgc aatgcaactg aatatggcaa
     1381 aacaatgtgt actcaactta ttaaagagtt agcaaacaat catcctgaat tgtgcatcat
     1441 tagtggatta gcttatggca tagatatttg tgctcacaaa gaagccttac acatgcactt
     1501 accaactgta gctgtattgg gtcatggttt agacagaatt taccctgctc aacatgctac
     1561 aatagccaaa
//
LOCUS       DAOPUR010000366         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_89932_length_1563_cov_3.470822, whole genome shotgun sequence.
ACCESSION   DAOPUR010000366 DAOPUR010000000
VERSION     DAOPUR010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89932_length_1563_cov_3.470822"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..>1563)
                     /locus_tag="PKN90_06380"
     CDS             complement(13..>1563)
                     /locus_tag="PKN90_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ85936.1"
                     /translation="EAIAQGAIDGGISGVYAYPGTPSTEITTYIQETTDKREIRSDWS
                     VNEKTAMEAAMGLSYVGKRALVCMKHVGLNVAADAFINAAITGINGGIVVLVADDPSM
                     HSSQNEQDSRFYGKFALIPILEPSNQQEAYDMAYTGFELSEKLGYPILMRITTRLAHS
                     RSGIIRKPQKQQRPISLPTNRKQFILLPAIARQRYSALLAAQSEFEEQANNSLYNEYI
                     DGVNKKMGIIACGLTFNYLMENFPNGCEYPIVKIGQYPLPRKKIERLIDECESILVIE
                     DGYPLVEELLKGYTGKGIQVKGKLDGTLPRQGELNPNNVGQAFGFTLEKDITIPEVVK
                     MRPPSLCKGCGHIDLYESLVEVLKDYPTARVFSDIGCYTLGALPPYTAIDTCLDMGAS
                     ITMAKGAADGGLHPSVAVIGDSTFTHSGITGLIDCVNDNSNVTIIIVDNETTAMTGGQ
                     TSSGTGKLEAICQAVGVDENHIHLCEPLKKNIDYIVQLIKKEISHTGVSVIIPRRECI
                     QTLKRKKK"
BASE COUNT          425 a          315 c          278 g          545 t
ORIGIN      
        1 tattttcata gtttatttct ttttacgttt taaagtttgt atacactctc tgcgaggaat
       61 aattacggac actccagtat ggctgatttc ttttttgata agttgtacta tgtagtcaat
      121 atttttcttt agtggttcgc ataaatgtat atgattttcg tcaactccta cggcttgaca
      181 aattgcttca agttttcctg tcccagacga tgtttgtcca ccagtcattg ccgttgtttc
      241 gttatcaaca ataatgatgg taacattgct attgtcattg acacaatcta ttagtccagt
      301 aatacctgag tgggtaaatg tagaatcacc aattactgca acagatggat gcaaaccacc
      361 atcggctgcc ccttttgcca tcgtaataga tgctcccata tccaaacaag tatcaatggc
      421 agtataggga ggcaatgctc ctaaagtata acaaccaatg tcgctaaaaa cacgagcagt
      481 aggatagtct ttgagcactt ctaccaatga ttcgtacaaa tcaatatgcc cacatccttt
      541 gcaaagcgat ggtggacgca tttttactac ttctggtata gtaatgtctt tttctagagt
      601 aaacccaaaa gcttgcccca cattattggg gttgagttct ccttgtcgag gtagcgtgcc
      661 atcaagtttt ccttttacct gtatgccctt tcctgtatag ccttttagta attcttccac
      721 taggggatat ccatcttcaa ttaccaatat gctttcgcat tcgtctatta acctttcaat
      781 ttttttacga ggcaatgggt attgtccaat ttttacaatg ggatattcac atccgttggg
      841 gaaattctcc atcaggtaat taaatgttaa cccacaggct attattccca tttttttgtt
      901 cactccatca atatattcat tgtatagtga gttatttgct tgttcttcaa attcactttg
      961 tgctgccaac aaagcactat aacgttgtct tgcaatggca ggtagtaaaa taaactgttt
     1021 tctatttgtg ggtaacgata tagggcgttg ttgcttttgt ggtttacgta taatgcctga
     1081 tcgggaatga gctaatcgtg tggtaatacg cattaatatt ggatagccta atttttctga
     1141 aagttcaaaa ccagtataag ccatatcata tgcttcttgt tgattactag gttcaagaat
     1201 tggaataagt gcaaatttac cataaaaacg tgaatcttgt tcgttttgag acgaatgcat
     1261 ggatgggtca tcagctacta aaactactat tcctccattt attcctgtaa tggcagcatt
     1321 aataaaggca tctgcagcaa cattcaagcc aacgtgtttc atacatacta aggctcgttt
     1381 tcctacataa gataaaccca tagcagcctc catagctgtt ttttcattta ccgaccaatc
     1441 gctacgtatc tctcttttgt cggttgtttc ttgaatatag gttgttattt cggtagatgg
     1501 ggtgcctgga taagcataca cacctgaaat accaccatca atagctcctt gtgctattgc
     1561 ttc
//
LOCUS       DAOPUR010000367         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_90055_length_1562_cov_2.402123, whole genome shotgun sequence.
ACCESSION   DAOPUR010000367 DAOPUR010000000
VERSION     DAOPUR010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90055_length_1562_cov_2.402123"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="PKN90_06385"
     CDS             <1..1413
                     /locus_tag="PKN90_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85937.1"
                     /translation="ANNTLYIDIPSILRRMQSVFTPEKIVWINSTRALWNNFETLILQ
                     AENKRNDLQFQQLVVNYTKKTEIDILEEKQADMLVENEQPNIEDTSTIHTTQSEKTET
                     TTPESASPTPAKKTRITALKTWELALTKPIVTSPQLVINQTTGLHEIMVQDTDNFLYL
                     ITSEGKLVWRIRIAGRIISDIHQVDMYRNKRLQMVFNTAEKLYIIDRSGKNVNNFPIN
                     FATKATAGVAVFDYNNNGDYRFFIPLANKKIALYKKDGSQPSDWKFKQSTALVNKPIQ
                     YFRSNGMDYLVASTNKKSYFLHRRGNERLIPEKPVISSSNNNFSLIYTGGIEKFVTTD
                     KNGNIVLINPNKKVDKVQLRTWSSQHAFTTFTHQKTNYYVFVDNKRIEIYDEEFNSVM
                     LYEDIQTKKPSIMLWENLLATYNEKSKEIVLVDLIARQVVANDIKTDKNLFYIGQLKP
                     YKKTCVVVSEKNKVIHYVLN"
BASE COUNT          608 a          302 c          260 g          392 t
ORIGIN      
        1 gccaacaata ccctttacat cgacattcct tctatactaa gacgtatgca atcggtattt
       61 acgcccgaaa aaattgtgtg gataaacagt acacgtgcat tgtggaataa ttttgaaaca
      121 ctcatcctgc aagcagaaaa taaacgaaat gatttgcaat ttcaacaact agtggtcaac
      181 tacacgaaaa aaacagaaat agacatattg gaagaaaaac aagcggatat gttagtagag
      241 aatgagcaac caaatataga agacactagt actatacata ccacccaatc agaaaagact
      301 gaaacaacaa cccccgaatc ggcaagccct acccctgcta aaaaaacaag gataacagct
      361 ctcaaaacat gggagttagc tcttactaaa ccaatagtta ctagccctca actggtcata
      421 aaccaaacaa ctggactaca cgaaataatg gtgcaagaca ctgataactt tttatattta
      481 atcaccagcg aaggaaaact cgtatggcgt attcgcattg ctggacgcat tattagcgat
      541 atccatcaag tggatatgta cagaaacaaa aggttgcaaa tggtgtttaa taccgccgaa
      601 aaactataca tcatcgaccg tagtggtaag aatgtaaaca atttccctat aaattttgcg
      661 acaaaagcta ctgctggagt ggctgtattc gactataaca acaatggcga ttatcgcttt
      721 tttattccac ttgccaacaa aaaaattgcc ctgtacaaaa aagatggctc acaaccaagc
      781 gactggaaat ttaagcaaag caccgcactc gtaaacaaac ctatacaata ttttagaagt
      841 aatggcatgg attatttagt ggcaagtacg aataaaaaaa gctatttctt acaccgcaga
      901 ggcaatgaac gattaattcc ggagaaacct gtaataagct cctcaaacaa taatttctca
      961 ctcatatata ctggaggaat agaaaaattt gttactacag ataaaaatgg aaatatagta
     1021 cttattaatc caaataagaa agtggataag gtgcaactac gcacttggag tagccaacat
     1081 gcctttacaa catttacaca ccaaaaaaca aactattatg tatttgtaga taataaacgc
     1141 atcgaaatat acgacgaaga gtttaacagt gtaatgctgt acgaagacat acagacaaaa
     1201 aaaccaagca ttatgctgtg ggaaaattta cttgctacct acaacgaaaa atcaaaagaa
     1261 atcgtattag tagatttaat agctcgacaa gtagtagcga acgatataaa aacggacaaa
     1321 aatctattct acatcggaca attaaaacca tacaaaaaaa cctgtgtagt ggtaagcgaa
     1381 aaaaacaagg taatacatta tgtattgaac taacaaggct tttttagtta ttcttcttgt
     1441 atattcgatg tataacggaa taaatttgtg gatcaaatat gaccttcgct atccaattat
     1501 gaatcaccgg ttcaccgtag ctcttaacct cgcactacac gctaagtgca tggcgtttct
     1561 tt
//
LOCUS       DAOPUR010000368         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_90056_length_1562_cov_2.390179, whole genome shotgun sequence.
ACCESSION   DAOPUR010000368 DAOPUR010000000
VERSION     DAOPUR010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90056_length_1562_cov_2.390179"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKN90_06390"
     CDS             <1..395
                     /locus_tag="PKN90_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85938.1"
                     /translation="ISNPSNYMSMLNSYLMHTIQFNKTNWQVSSSFNYTYSDMQQASN
                     TMFGPTVAVSARFLNKTLQSGLSYSCNWSYTEAEYDQFVTNIRIHSSYTFLKKHTLQA
                     SGTYQLLAKNTTPNIYRTNLTIAYRYTF"
     gene            398..>1562
                     /locus_tag="PKN90_06395"
     CDS             398..>1562
                     /locus_tag="PKN90_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85939.1"
                     /translation="MRNKQHTPALPYNKSKLKERHSFILYLIPFFVFLFCNSLGLYAQ
                     QASKNEANDVLTAIESSQRYIDKLSINDISVLPVGMQKTIGNIKVIIAVYDITYHSEY
                     ASMSVYAKMLIPQGDGTELFFGMEDVKLSYDGGIIGDAKLKLLGDITIPFNSNTKLVL
                     KGSFQPKTEIKEDLTYAVLTCDGISEIGVSGEVQFATSFITKVNNETATNEAVIGAFK
                     TVITDWNDLLVNINLPSFAIVGLDDYIFSCNNVVLDFSDTRNAQGMVFPETYKELYLP
                     TGNETLWRGVYATAISMKLPEQFTRSDKPKERISIDAQHLIFDHNGVSGLFATNYAIL
                     PYDNGTASGWNFSVERFRLGMLANRVSEASFGGAIGIPLGEKCKLEYNATIDAS"
BASE COUNT          522 a          273 c          295 g          472 t
ORIGIN      
        1 gcataagcaa tccatcaaat tatatgtcga tgctcaattc ttacctgatg catacgatac
       61 aatttaacaa aaccaactgg caggtaagca gttcgtttaa ttatacctat tcagatatgc
      121 aacaggcttc aaacacgatg tttggtccta ctgtagctgt ttcggcaaga tttctaaaca
      181 aaacacttca gtcgggctta tcatattcgt gcaattggtc gtacacagaa gctgagtatg
      241 accagtttgt aaccaatatt cggatacatt cgagttatac atttctcaag aaacacactt
      301 tacaagcaag tggcacatat caattattag ccaaaaatac aacaccaaat atttatagaa
      361 ccaatttaac catagcttat agatatacat tttaactatg agaaataaac aacacacacc
      421 tgcattgcca tacaataaaa gtaagttgaa agagagacac tcttttatat tgtatttgat
      481 accatttttt gtatttcttt tttgtaactc gctaggttta tatgctcaac aagctagcaa
      541 gaatgaagct aatgacgtgc taacggcaat agaatcatca caacggtata ttgacaaatt
      601 atccataaat gatataagtg tattgcctgt tggtatgcaa aaaaccattg gaaacataaa
      661 agtaatcata gcggtgtatg atatcacgta ccactcagaa tatgcttcga tgagtgtgta
      721 tgcaaaaatg cttattccac aaggcgatgg cactgagttg ttttttggta tggaagatgt
      781 aaaattgtca tacgatggag gaattatagg tgatgccaaa ttaaaactat tgggcgatat
      841 tactattccg ttcaatagta atacaaaatt ggtattgaag ggctcattcc aacccaaaac
      901 agaaataaaa gaagacctta cttacgcggt gttaacatgc gatggtattt cagaaattgg
      961 ggtaagcgga gaagtgcagt ttgcaacatc atttataacc aaagtgaata atgaaaccgc
     1021 tacaaatgag gcagtaattg gtgcatttaa aaccgtaatt actgattgga atgatttgtt
     1081 ggtgaatatt aatttgccaa gttttgcaat tgttgggctt gacgattata tattttcttg
     1141 caataatgta gtgttagatt ttagcgatac acgcaatgca caaggtatgg ttttccctga
     1201 aacctataaa gaactatatt tacctacagg caatgaaaca ttatggcgag gcgtgtatgc
     1261 tactgccata agcatgaaac tgcccgaaca atttactcgc tcagataaac ccaaagaacg
     1321 tatttcgatt gatgctcaac atcttatttt tgaccataat ggggtatctg gtctttttgc
     1381 cactaactat gccatactgc cgtatgataa cggaacggcg tcaggctgga atttttctgt
     1441 cgaacggttt aggttaggga tgttagctaa tagagtttcc gaagcctcat ttggaggagc
     1501 tattggaatt ccccttggcg aaaaatgtaa attggaatat aatgctacta tcgatgcctc
     1561 ca
//
LOCUS       DAOPUR010000369         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_90293_length_1559_cov_3.405585, whole genome shotgun sequence.
ACCESSION   DAOPUR010000369 DAOPUR010000000
VERSION     DAOPUR010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90293_length_1559_cov_3.405585"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..240)
                     /locus_tag="PKN90_06400"
     CDS             complement(31..240)
                     /locus_tag="PKN90_06400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ85940.1"
                     /translation="MAFVFAWNRRRNINLLPADTTLFVSIILSVRNEEKAIQSLLYSM
                     QNQTYKNFELRIVDDSSEDETFSLC"
     gene            complement(286..897)
                     /locus_tag="PKN90_06405"
     CDS             complement(286..897)
                     /locus_tag="PKN90_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNZ85941.1"
                     /translation="MIGILTSIPVGPIAMLCIQKTLNKGHMHGFFSGLGAATSDTTYA
                     MVALFGLSFVMPFIEQNQLLIQIIGSIVILLFGFFIFSKNPVAVLNKNIATKESYLQD
                     YLASLLLTLSNPLIIFLFLGLFARFSFLSNTDNTVYSLSGIIAVFAGASTWWFVLTFV
                     ASIFRKKINIRGLGVINKLTGSVIIVLALGGLILSILGKSVNV"
     gene            1064..>1559
                     /locus_tag="PKN90_06410"
     CDS             1064..>1559
                     /locus_tag="PKN90_06410"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNZ85942.1"
                     /translation="MNKKIYFVSDAHLGSLAYTNDTYREKKLVAWLDAIKHDAAAIYL
                     VGDIFDFWYEYKYVIPKGFVRLFGKIAELCDSGIPIHFFIGNHDIWTYGYLEKELGMQ
                     VHKQPLLTEFNGKRFYVAHGNGLGKNPFVVQMIEKIFHSTSVRFFYNMIHPSINMRLG
                     LAWSK"
BASE COUNT          580 a          274 c          233 g          472 t
ORIGIN      
        1 ttaaaatttt acagaaggtc acataaaatt ttaacacaaa gaaaacgttt catcctcaga
       61 agaatcatca acaatacgca attcaaaatt tttatatgtc tgattctgca tactataaag
      121 taacgactgt atcgcttttt cttcatttct tactgaaagt ataatagata caaaaagagt
      181 agtatctgct ggcaaaagat tgatattacg ccttctgttc caagcaaata caaatgccat
      241 gattagacct atatacggga tgagaaacca ccataaccac atactttaca cattaacaga
      301 tttacctaat atcgacaaaa tcaacccccc taaagctaat acaataatta cacttcccgt
      361 aagtttgtta ataaccccaa gtcctcttat gttgattttt ttgcggaaaa tactagcgac
      421 aaatgtcagc acaaaccacc aagtactggc tccagcaaaa acagctataa taccagaaag
      481 ggaataaact gtattgtcgg tattgctcaa aaaactaaaa cgtgcaaaca accccaaaaa
      541 taaaaaaata atcaaagggt tacttaacgt taataataat gatgccaaat aatcttgcaa
      601 ataactttct tttgtagcaa tatttttgtt aagaacggca acgggatttt ttgaaaaaat
      661 aaaaaatcca aataataaaa ttacaatact acctattatt tgaattaaca attgattttg
      721 ttcgataaag ggcattacaa atgataatcc gaacaatgcc accatagcat aggtggtatc
      781 agaagtagct gctcccaacc cagaaaaaaa accatgcata tgccccttat tcaatgtttt
      841 ttgaatacac aacatggcta taggacctac aggtatggag gtcagtattc caatcaaaaa
      901 cccttttact aaaatatcaa acatacctgc aaatatctat aaaaaaaaac agaaatctga
      961 ctatcattaa gtttttttta atttaagagg ctaaaagtag gaaaaattct gttacttttg
     1021 tcaagaaaca cataaattat acttcctcat tacatattta gcaatgaata aaaaaatata
     1081 ttttgtatca gatgctcatt taggctcatt agcgtataca aatgatacgt atcgggaaaa
     1141 aaaacttgtt gcatggttag atgcaatcaa acatgatgct gctgccatat acttggttgg
     1201 agacattttt gatttttggt acgaatacaa atatgtgata ccgaaaggat ttgttcgctt
     1261 gtttggcaaa attgccgaat tgtgtgatag cggcattcct attcattttt ttattggcaa
     1321 tcacgatatc tggacatatg ggtatttgga aaaagaatta ggcatgcaag tccataaaca
     1381 accattatta acggaattca atggcaaacg attttacgtt gcccatggca atgggttagg
     1441 aaaaaaccct ttcgtagtgc aaatgattga gaaaatattt cactctactt ctgtccgttt
     1501 tttttataat atgatacatc cttccattaa catgcgattg gggttagctt ggtcgaaaa
//
LOCUS       DAOPUR010000370         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_90907_length_1553_cov_2.365821, whole genome shotgun sequence.
ACCESSION   DAOPUR010000370 DAOPUR010000000
VERSION     DAOPUR010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90907_length_1553_cov_2.365821"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..>1553
                     /locus_tag="PKN90_06415"
     CDS             348..>1553
                     /locus_tag="PKN90_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85943.1"
                     /translation="MEKTFLKSKRFIRFLLVLCCFLSISASILAQNLTNKAIDKSASG
                     FGDLLGSTTFSLADDNNATTQPNFTIGEIFSFPTGMSTITLNSIYTNGYGSWGTGGTN
                     TAPRYAIVSNPNVLNSGYKSITDGVNRLVMRTPNKSTEMFRFRVEGHKPGDPITVEFT
                     MQEITSNTVGSVTPQLIINIKEVGGNDYNYYHKLAQGGTLNFSQTIARNSGAIEVIVS
                     RDNWNEGANFVYAISNIKVYGTSYQYIDALNSTVCDGDNVTLTAEGFEAFSGDYTWQS
                     SSTETGPFTTIAGEISSTINVDALLGNTFYKVTRGTATSEVFRLNARVCCKETAGQQI
                     IFKESFGNVPSGTRKNFTDIGTTGIVPLEYKATKNVNDGEYAIVSKTKDAEDSSEGIG
                     HPWYWAGTDH"
BASE COUNT          545 a          266 c          272 g          470 t
ORIGIN      
        1 ttcgtatagt tctttgccca ttcctacaaa ctgagctcct tgaccaggaa atacaaatgc
       61 tttcattttg attattttaa attaaacaac taaaaaaata gcatttttca tctgcaattc
      121 tacattttta gctaagaata aagctagagc aaatgcatca gataatgcta attaacgtat
      181 gtaaattttt cgagtgcaaa aatactcttt ttatcatcat actcaaaaaa aacataaaaa
      241 aagcttgtta aatacaaaaa cttgtactta ctttgcacct tattctaaac ataaatagct
      301 tgttagcaaa ttattacaaa ttcttttaac ctaaacttga ttttattatg gaaaaaacat
      361 tcctaaaatc caaacgattt attcgttttt tgcttgtctt atgttgcttt ttgagcatat
      421 cagcaagcat cttagctcaa aatcttacca acaaagccat tgacaagagt gcatctggtt
      481 ttggcgattt acttggatcc accacgtttt cattagcaga tgataacaat gctacaacac
      541 aacccaattt taccatcggg gaaatatttt cttttccaac gggaatgtca actattacat
      601 taaacagcat atacacaaat gggtatggca gttggggtac aggaggcaca aatacagctc
      661 cacgttatgc cattgttagc aatccaaatg tattgaatag tggctacaaa tcaattacgg
      721 atggtgtaaa tcgtttggtt atgcgtacgc ctaataaatc aaccgaaatg tttcgcttta
      781 gagtagaagg acataagcca ggagacccta ttactgttga atttacgatg caagaaataa
      841 ctagtaatac agtaggtagc gtaactccac aactaattat caacataaaa gaagtaggtg
      901 gaaatgacta caattactat cataagttag cacaaggagg cacattaaat ttttcgcaaa
      961 ctattgctag aaacagtggg gcaattgagg taattgttag tagagataac tggaatgaag
     1021 gtgcaaattt tgtgtacgct atcagcaaca ttaaggttta tggtacatcc tatcaatata
     1081 tagacgcact taattctact gtatgtgatg gagacaatgt tacacttaca gcagaagggt
     1141 ttgaggcttt cagcggtgat tatacatggc aatcgtcttc aacagaaaca ggtcctttta
     1201 caactattgc aggtgaaatt tcctctacta ttaatgttga tgcattatta ggcaatacct
     1261 tttataaagt aactagagga actgctactt ctgaagtatt tcgattaaat gcacgtgtat
     1321 gctgtaaaga aacagcagga caacaaataa tttttaaaga aagctttggc aatgttccta
     1381 gtggaacaag aaagaatttt actgacatag gaactactgg tattgttcca ttagaataca
     1441 aagccacaaa aaatgtgaat gacggagagt atgccattgt gtcaaagaca aaagatgcag
     1501 aagactctag cgaaggtatt ggtcatccat ggtactgggc aggcactgat cac
//
LOCUS       DAOPUR010000371         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_91379_length_1547_cov_4.096515, whole genome shotgun sequence.
ACCESSION   DAOPUR010000371 DAOPUR010000000
VERSION     DAOPUR010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91379_length_1547_cov_4.096515"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PKN90_06420"
     CDS             complement(<1..1529)
                     /locus_tag="PKN90_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85944.1"
                     /translation="MPRGRQYGYIYTKDLNASKIGSILAHELAHGKFKLYHTFDNIYA
                     GKLAQGSTTNLMDYSGGTHLAKWQWNQLYDPAILNGVFDRDEDGEYSTDGHYYTVQLV
                     ALMMGFEFDDAYKLGAEAENPDSYVKNPWDMEERYTWMKAGLQQEYHALTGRKHGIEL
                     ALTTYALLITHKNESNFHQYLLHRFGDCFGHINTRSTTFNTNVDLNDYVKAVDKLFND
                     YVKPKIKNVNKDNKIIVDMSYSVNHSIKIEKTMEFLKIYYDGKNLLTTLSIEDTKKEL
                     IQQLLHSYSEDVNLNFSHNLYLRNELTAQLYKLLPTAIQNDYDMYGNDYTGTKGHSID
                     SELLSKLYNANPDDIGGRRELYFYYVNKLIDLLTVKYSKQNINKAKIIKHIQTIINNA
                     TDGTYEKRLDGILAFECEYYKNPNSTSYEFYIPIKYNPSIMLDSEKAFDALINNFSND
                     SYKQRDIMLSYLANRLIIDKIAWKPENVYVSGGLDRNNYPKYVYTHKPEVITNGKYDI
                     S"
BASE COUNT          453 a          266 c          244 g          584 t
ORIGIN      
        1 attgatatat catattttcc attagtaatg acctcaggtt tgtgtgtata aacgtatttt
       61 ggataattat ttctgtctaa acctccactt acataaacgt tttcaggttt ccatgcaatt
      121 ttatctatta ttaatctatt agctaaataa gacaacataa tatctctttg cttataagaa
      181 tcatttgaaa aattattaat aagagcatca aaagcttttt ctgaatctaa cattatagat
      241 ggattatatt ttattggtat atagaattca taacttgttg aattgggatt tttgtaatac
      301 tcacattcaa aagccaatat tccgtctaat cgtttttcgt aagttccatc ggtagcattg
      361 tttataattg tttgaatatg tttaatgatt tttgctttat taatattttg ttttgagtat
      421 ttaacagtca acaaatcaat taatttgtta acataataaa aatataattc tcttcttcct
      481 ccaatatcgt caggattagc attatacaat tttgaaagta attcagagtc gattgaatgc
      541 ccttttgtac cagtataatc atttccatac atatcgtaat cattctgaat tgcagtaggt
      601 aaaagtttgt aaagttgtgc tgttaattca tttcgcaagt acaaattgtg cgaaaagttt
      661 aaatttacat cctccgaata gctatgcaat aactgctgta ttaattcttt cttggtatct
      721 tcaatcgaga gcgttgtcaa aagattttta ccatcgtaat atatttttag aaattccatc
      781 gttttttcaa tttttatcga atgattaaca gaataactca tatcaactat aattttattg
      841 tctttattaa cgttttttat cttgggtttc acataatcat taaacaattt atctactgct
      901 tttacataat catttaagtc aacatttgta ttaaaagtag ttgaccttgt gttgatatgt
      961 ccgaagcaat caccaaaacg atgtaacaaa tactgatgaa agttcgattc gtttttatga
     1021 gttatcaata atgcataggt ggttaaagct aattcaatac catgttttct tcctgtcagt
     1081 gcgtggtatt cttgctgtaa acctgctttc atccaagtat aacgttcttc catatcccaa
     1141 gggtttttta cgtatgaatc aggattttca gcttcggcac ccaatttata agcatcgtcg
     1201 aattcaaatc ccatcatcaa tgctactaat tgtactgtat aataatgtcc atctgttgaa
     1261 tactccccat cctcatccct atcaaacacc ccattgagta tagcggggtc gtagagttgg
     1321 ttccattgcc atttggctag gtgggtgcct ccgctgtagt ccattaggtt ggtggtgctg
     1381 ccttgggcga gtttgcctgc gtatatattg tcgaaggtat ggtacagctt aaacttgcca
     1441 tgggctagtt cgtgggctaa gatgctgcct atcttgctag cgtttaagtc tttggtatag
     1501 atatacccat actgcctgcc acggggcata aagcctacta catcgcg
//
LOCUS       DAOPUR010000372         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_91649_length_1544_cov_2.946273, whole genome shotgun sequence.
ACCESSION   DAOPUR010000372 DAOPUR010000000
VERSION     DAOPUR010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91649_length_1544_cov_2.946273"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..898
                     /locus_tag="PKN90_06425"
     CDS             194..898
                     /locus_tag="PKN90_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85945.1"
                     /translation="MTTKKYILLIASLLLSTAYVAANKQTLSTIELSVEKGKSYNYPT
                     FALWLESAGGKFVQELYVTQSLATGIYNNADAGDKRWKREKGEARRPSTLPYFLHKRD
                     IQAPDGTYLPTPEQPIPDAYTGATPRQSDVMTIVLDRQLYGKYRIVFEINQPWDFNRV
                     WYNNRYPNNTDYLLSGQPSLVYMVEIDTNNLQEEYTLNPIGHGHPYGKTGTLFTDISG
                     ITTAKNIFKSIKVKVY"
     gene            complement(959..1168)
                     /locus_tag="PKN90_06430"
     CDS             complement(959..1168)
                     /locus_tag="PKN90_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85946.1"
                     /translation="MKKLLCPQCRIHRFRVFNAARESVVVTVNEHYEVIPIHEGVSLE
                     GYFTDELHCLGCSWIGSPKALLNRY"
     gene            1284..>1544
                     /locus_tag="PKN90_06435"
     CDS             1284..>1544
                     /locus_tag="PKN90_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ85947.1"
                     /translation="MKTALIIGSTGLVGSALLNELLAHPAYSKVISFTRKPSGNTHPK
                     LHEHCIDFNKIASYKDLIQGDDLYCAMGTTIKKAGSQAAFTLV"
BASE COUNT          518 a          351 c          291 g          384 t
ORIGIN      
        1 ccgaccaata catcaacgag gacaaagatg gaaatgtggt aatcgaaaac ccaacgtcac
       61 cgctcaacga gcaacggtat gtattacgca aactcgaaaa tacaggcagt ggcacagtat
      121 tgccttccat tggtattatg atagaatttt aattacataa cacaactaaa tagctcacta
      181 aaaacgaact cgtatgacta caaaaaaata tatactactt attgctagcc tactgctaag
      241 cacagcttat gtggcagcaa acaagcaaac gctatcaacc attgaactga gtgtggaaaa
      301 aggcaaaagt tacaactacc ctacttttgc gttgtggctc gaaagtgctg gaggcaaatt
      361 tgtgcaagaa ttgtacgtta cacaatccct agctacaggc atctacaaca atgctgacgc
      421 tggcgataaa cgctggaaaa gagaaaaagg agaagctcga cgcccgtcaa cactgcctta
      481 ttttttgcac aaacgcgata tacaagcacc tgacggcacg tacctaccaa ctcccgaaca
      541 acccataccc gatgcttata caggagctac tcctcgccaa tctgacgtaa tgaccatcgt
      601 attagacaga caattatacg gaaaatacag aatagtgttt gagattaacc agccttggga
      661 ttttaaccgt gtgtggtaca acaaccgcta cccaaacaat accgactacc tgctttcggg
      721 gcaaccatcg ttggtgtata tggtagaaat agatacgaac aatttgcaag aggaatacac
      781 tctcaaccca attggtcatg gacatccgta tggtaaaaca ggcaccttat ttacagatat
      841 ttctggcatt accactgcca aaaacatatt taaatcgatt aaagtaaagg tatattaagc
      901 acacacaaag cccaataagt gcaatgagag gaagagcaat ttttctctca ttgttgtatc
      961 aataccgatt gagtaaggct ttgggcgaac ctatccacga gcaacccaaa caatggagtt
     1021 cgtcagtaaa atacccctcg agcgatacac cctcgtgaat gggtataacc tcatagtgtt
     1081 cgtttacagt tactaccacc gactcgcggg ctgcattaaa aacacgaaac cgatggatac
     1141 gacattgtgg gcaaaggagt tttttcatac tagtaaaaac attaatttgg tgcaaagata
     1201 ctttttcttc gtatatacac gcatatcaaa tgcttacttt gcattatttt gcgtactttt
     1261 gatagcaaat aattcaccca tttatgaaaa cagcacttat tattggtagc acaggattgg
     1321 ttggttcggc tttgctcaac gaactattgg cacatccagc ctacagcaag gttatttcat
     1381 ttacccgcaa acccagtgga aatacgcacc caaaactaca cgaacattgc atcgacttta
     1441 acaagatagc ctcgtacaaa gacttgatac aaggcgatga cttgtactgt gccatgggta
     1501 ccaccatcaa aaaagcaggt agccaagccg catttacttt ggtt
//
LOCUS       DAOPUR010000373         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_93406_length_1526_cov_2.063222, whole genome shotgun sequence.
ACCESSION   DAOPUR010000373 DAOPUR010000000
VERSION     DAOPUR010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93406_length_1526_cov_2.063222"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKN90_06440"
     CDS             complement(<1..378)
                     /locus_tag="PKN90_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNZ85948.1"
                     /translation="MNLLKNNIATFFKGFAMGTANVIPGVSGGTIALILGIFERIIDS
                     LKCFDGEAIKLLIKGKFKAFATHTDLGFLSVVLLGVIVSIFTVAKLLSFLFLNYPIFI
                     WSYFFGLIIASIWLVGKTISSYNT"
     gene            complement(508..918)
                     /locus_tag="PKN90_06445"
     CDS             complement(508..918)
                     /locus_tag="PKN90_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNZ85949.1"
                     /translation="MEELTLTTPSLLFSAVSLLLLAYTNRFLSYAQVVRTLKEKYTKD
                     HSAITGAQINNLRKRLYLTRAMQVQGIVSLFLCVVTMLLIYIQLQLIAAYVFGVALFL
                     LILSLGTSIWEIYISVKALEIYLSDITNELDNKK"
     gene            complement(1025..>1526)
                     /locus_tag="PKN90_06450"
     CDS             complement(1025..>1526)
                     /locus_tag="PKN90_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNZ85950.1"
                     /translation="KEIETTHDCKIPVIAAGGIYTGANMYDIMKLGADGVQLGTRFVT
                     TTECDASEIFKQAYIDATEADVEIIKSPVGMPGRAIFSDFLQKVKDGEKQPKSCPFHC
                     IRTCQIETSPYCIMLALFNAYKGNMTMGYAFAGTNAHLATAIVTVKDTMDAIQEEYKA
                     REKELV"
BASE COUNT          496 a          276 c          258 g          496 t
ORIGIN      
        1 ggtattgtag ctagaaatag ttttacccac cagccaaatt gaagcaatga ttaaaccaaa
       61 aaaatacgac caaataaaaa tggggtaatt aagaaaaaga aagcttaaca gttttgcaac
      121 tgtaaagata ctgacaatta ctcctaacag tactactgat aaaaaaccca agtctgtatg
      181 tgtagcaaat gctttaaatt taccctttat caataattta atagcctcgc catcgaaaca
      241 ttttaagcta tcaataattc gttcaaaaat acccaatata agagctattg tgcctcctga
      301 tacccctggt ataacattag cagtacccat agcaaaacct ttaaagaaag tagcgatgtt
      361 gtttttaagc agattcatgt gtgtttttat tagaaatgtg gctgatagaa atatattgaa
      421 atacaaagca caaaagtagc aatagtttat tgaaaaacaa gtaaataaaa atgacttatt
      481 atagtctatg gaataatttt ttaaacatta cttcttgtta tccaattcgt tggttatgtc
      541 gcttaggtag atttcgagag ctttaacgga tatgtatatt tcccaaatcg aagttcctaa
      601 agagaggatg agtaaaaata aagctacccc aaacacataa gcagctatga gttgtaactg
      661 tatgtatatc agcaacatag taacaacgca taaaaataaa cttacaatgc cttgcacttg
      721 catcgctcgt gtgaggtaca agcgtttacg taagttgtta atttgtgccc ccgtgatggc
      781 tgaatgatct ttggtgtatt tttctttcag cgttcgtacc acttgggcat acgacaaaaa
      841 acgattggta tatgccaaca gcaataacga tactgcagaa aaaagcaatg aaggtgtagt
      901 taatgtaagt tcttccatat ttacgttata gtaggtttta cgaaatgaat ccacttttac
      961 cacactagtt gtttttgtta actgttgtgg tagtagtatt tgcttacaaa aatacaattt
     1021 tttattaaac tagctctttt tctctagctt tgtattcttc ttggatagca tccatggtat
     1081 cttttacggt aacaattgct gtagccaagt gtgcattcgt gccagcaaat gcgtaaccca
     1141 tggtcatatt gcccttgtat gcattaaaca gagccaacat aatacaataa gggcttgttt
     1201 ctatttggca ggttctaata caatggaaag gacaactttt cggctgtttt tcgccatctt
     1261 tcactttttg taaaaaatcg ctaaagatcg ctctaccagg cattcccact ggacttttta
     1321 ttatctctac atccgcttcg gtagcatcaa tatacgcttg tttaaaaatt tctgaagcat
     1381 cacattcggt cgtggttaca aaacgtgttc ctagttgaac cccatcagca cctaatttca
     1441 tgatgtcata catgtttgca cctgtgtaaa tacctccagc agctataaca ggaattttgc
     1501 agtcgtgtgt tgtttctatt tcttta
//
LOCUS       DAOPUR010000374         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_93571_length_1524_cov_3.316542, whole genome shotgun sequence.
ACCESSION   DAOPUR010000374 DAOPUR010000000
VERSION     DAOPUR010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93571_length_1524_cov_3.316542"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PKN90_06455"
     CDS             complement(<1..137)
                     /locus_tag="PKN90_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ85951.1"
                     /translation="MKPKKIEHIGIAVPSIAEALPFYENVLGLTCYAVEEVADQKVKT
                     A"
     gene            complement(150..1226)
                     /locus_tag="PKN90_06460"
     CDS             complement(150..1226)
                     /locus_tag="PKN90_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85952.1"
                     /translation="MVYIVLFLLLLALMNVYFSLGRKYGIVAHVNERSSHKKPVITGA
                     GIIFVVSYLFYLVYASWKGIHVNWFISIAALLIAIVSFFDDIKEIWFLFRLCFQIVAV
                     ILLMWQLKIDFSISFLGLKMLDWIAFSFLLILFVAAVNLYNFIDGINGLIAGLSLVFF
                     FSVIFIDYYVIDFIENREKIYFLIVPTIIFSLYNFRKKPVCFCGDVGSIFLGIIVVYF
                     LASLIIKTNNFTYVLLFSLIIIEAGYTVIQRLLMGQNIFKPHRIHLFQLLCNQYGHSH
                     LKVTLFYVLIQTVLSLFVVLANYYDLSITWQYSVGAVVFFMLSLSYVLYKRKLQGGHL
                     LEGGELIRKLRKIVYKKESNIYIK"
     gene            complement(1248..>1524)
                     /locus_tag="PKN90_06465"
     CDS             complement(1248..>1524)
                     /locus_tag="PKN90_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ85953.1"
                     /translation="SGSLFVETSTDKKNRSSAQGLFMMMTNGFGAIFGSLASGYIIDT
                     YFIQADGLKNWQGIWLTFSGYALVITILFALIFRYKHTTNDVLKIKH"
BASE COUNT          607 a          272 c          179 g          466 t
ORIGIN      
        1 aaagctgttt ttactttttg atcagctact tcttcaactg cgtaacaggt taatcctaat
       61 acgttttcat aaaaaggcaa agcctcggca atacttggaa cagctatgcc gatgtgttca
      121 attttttttg gtttcatttt tttaaaattt tatttaatat atatgttaga ttcttttttg
      181 taaactattt tgcgcaactt tcgtataagt tcgcctcctt ctaataaatg tcctccctgc
      241 aattttcgtt tgtataatac ataactcagg ctcaacataa aaaagaccac agctcctaca
      301 ctgtattgcc aagttatact caaatcataa taattagcta aaacgacaaa caaagaaagt
      361 acagtctgta tcaagacata aaaaagtgta acctttaaat gactatgccc gtattgattg
      421 cataataact gaaataagtg tatgcgatgt ggtttaaaaa tattttgtcc catcaataag
      481 cgttgaatga cagtatatcc tgcctcaata ataatgagag aaaataacaa aacatacgta
      541 aagttgtttg tctttataat caatgatgca agaaaataca ccacaattat acctaaaaaa
      601 attgaaccaa catccccaca aaagcaaact ggtttttttc taaaattata caatgaaaaa
      661 ataattgttg gaacaattaa gaaataaatt ttctctctat tttcgataaa atcaatcaca
      721 taatagtcaa taaaaatgac agagaaaaag aacactaaac ttaaacccgc aatcaaacca
      781 ttaattccat caataaaatt atataaattt acagcagcta caaaaagtat aagtaaaaaa
      841 gaaaaagcaa tccaatctaa cattttcaat cctaaaaaag aaatagagaa atctattttc
      901 aattgccaca tcaacaaaat aacagctact atttgaaaac acagccgaaa taaaaaccaa
      961 atttctttga tatcatcaaa aaaactaaca atggcaatta acaaagctgc aattgaaata
     1021 aaccaattta cgtgtatccc cttccacgaa gcatagacta agtaaaacaa ataagacaca
     1081 acaaaaataa taccagctcc tgttatcacg ggttttttat gagaacttct ttcattaaca
     1141 tgtgctacaa taccatattt tcttcctaac gaaaaataca cattcattaa tgcaagtaaa
     1201 agtaagaaaa ggactatata taccatataa tttctctatg ttaatgttta atgtttaatt
     1261 tttaatacat cattggttgt atgtttgtat ctaaatataa gagcaaacaa tattgtaata
     1321 accaaagcat acccagaaaa tgttaaccat ataccttgcc aattttttaa tccatctgcc
     1381 tggataaagt aagtatcaat aatgtatcca cttgccaagc taccaaatat tgcaccaaat
     1441 ccattggtca tcatcatgaa aagaccttgt gcactggaac gatttttttt atcagtagat
     1501 gtttcaacaa acaacgagcc cgaa
//
LOCUS       DAOPUR010000375         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_95352_length_1506_cov_2.519642, whole genome shotgun sequence.
ACCESSION   DAOPUR010000375 DAOPUR010000000
VERSION     DAOPUR010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95352_length_1506_cov_2.519642"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PKN90_06470"
     CDS             complement(<1..148)
                     /locus_tag="PKN90_06470"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNZ85954.1"
                     /translation="MNIEEVRTYCLSKPYVTEDFPFDETVLVFRVAGKIFVLCALDAM
                     PPRIN"
     gene            complement(163..531)
                     /locus_tag="PKN90_06475"
     CDS             complement(163..531)
                     /locus_tag="PKN90_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNZ85955.1"
                     /translation="MAIHNKLGEEGEKLATAYLQQKGYRIRHTNWRSGHLELDIVAES
                     HDTLYVIEVKTRSTAYFEHPKDAITNAKIKRIVHATEEYIHRFDIHKDIQFDIMALLP
                     NKHGTFDIEHIPDAFLAPLS"
     gene            629..844
                     /locus_tag="PKN90_06480"
     CDS             629..844
                     /locus_tag="PKN90_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85956.1"
                     /translation="MKKPSKRNDKNTQKSLLRKNRVTFVLNDKELNALEAYCKRFRVK
                     NKSRFVRTVLMSTVIERLEQSSPTLFD"
     gene            857..>1506
                     /gene="rmuC"
                     /locus_tag="PKN90_06485"
     CDS             857..>1506
                     /gene="rmuC"
                     /locus_tag="PKN90_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNZ85957.1"
                     /translation="MEWMWISIGLVSVLAVLWLIVKYMKQLMHNKLAEQEKALASSIA
                     VLEKEKALAQMQLADVQNVYQQTLQQLSKETEKYQQLSSQYAVLQTQNTHLSERLDTQ
                     KKELTELQQHFTKEFENIANRILKQNSSDFTDVNVKHLTDIVNPLKEKISLFEKKVED
                     AYDKELRDKLSLREEVRKLTELNTRISEEATNLTKALKGDVKKQGNWGEIVLERVL"
BASE COUNT          531 a          252 c          279 g          444 t
ORIGIN      
        1 cattgatgcg aggaggcatg gcatcaagag cacacaatac aaatattttt ccagcaactc
       61 taaataccaa tacggtctcg tcaaaaggaa aatcctcagt aacataaggc tttgacaaac
      121 aatatgttcg tacttcttca atgttcattg tctaagcttt ttttatgaaa gtggggctaa
      181 aaatgcatct ggaatatgtt caatatcaaa tgttccatgc ttattaggca gtagagccat
      241 aatgtcaaac tgtatatctt tgtggatatc aaaccgatga atgtattctt ctgttgcatg
      301 cacaatgcgt tttattttgg cattggtaat cgcatcttta ggatgttcaa aatacgctgt
      361 gctgcgtgtt tttacttcaa taacataaag cgtatcgtgc gattcagcca caatatccaa
      421 ttctaaatgt ccactacgcc aattggtatg gcggatacga tacccttttt gttgcaaata
      481 tgcagtagct aatttctctc cttcttctcc cagtttgttg tgtatcgcca tttattttac
      541 aaatttatta cgttttttct taaaagcatt tttttaatta tataaaccgt tgtacttttg
      601 caaggtagat agaaaaaaaa tctagtgaat gaaaaaacct agcaaacgta acgacaaaaa
      661 tacacaaaaa tcattactca gaaaaaatag agttactttt gtgttaaacg acaaagaatt
      721 aaatgcgttg gaggcatatt gtaaacgttt tagggtgaaa aataaatcac gttttgtaag
      781 gactgtttta atgtcaacgg ttattgaacg actagaacaa agtagtccaa ctttgtttga
      841 ttaaaaaaga tatacaatgg aatggatgtg gataagtatt gggttagtaa gtgtgcttgc
      901 tgtgctatgg cttattgtaa agtacatgaa acagcttatg cacaataaat tagccgagca
      961 agaaaaagct cttgccagtt ctattgcggt attagaaaag gagaaagcct tggctcaaat
     1021 gcaactggct gatgtgcaaa atgtgtatca gcaaacctta caacaactta gtaaagaaac
     1081 tgaaaaatac caacaactat cctctcagta tgctgtgttg caaacacaaa atacgcactt
     1141 gtccgaacga ttagacacgc aaaaaaaaga acttaccgaa ttgcaacaac attttaccaa
     1201 agagttcgag aacattgcca atcgcatttt gaaacaaaat tcttctgatt ttacagatgt
     1261 aaatgtaaaa caccttaccg atattgtcaa tccattaaaa gaaaaaattt ctttgttcga
     1321 aaagaaagtg gaagatgctt acgacaagga gcttagagac aagctaagtt tgcgtgaaga
     1381 agtacgaaaa ttaactgagc taaatacgcg tatcagcgaa gaagccacta atctgacgaa
     1441 agctttaaaa ggtgatgtaa aaaagcaagg taactggggc gaaattgtcc tcgaaagggt
     1501 attgga
//
LOCUS       DAOPUR010000376         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_97012_length_1490_cov_2.967247, whole genome shotgun sequence.
ACCESSION   DAOPUR010000376 DAOPUR010000000
VERSION     DAOPUR010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97012_length_1490_cov_2.967247"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..740)
                     /gene="efp"
                     /locus_tag="PKN90_06490"
     CDS             complement(177..740)
                     /gene="efp"
                     /locus_tag="PKN90_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNZ85958.1"
                     /translation="MATTADIRNGMCIEFNNGLYTIVEFLHVKPGKGAAFVRTKMKNV
                     QTGRILENTFNAGVKLDEVRIERRPYQFLYKDDMGYNFMHNETFEQISIDKDKINGVD
                     FLKEGDIAEVTVHADTETILFADLATTTILEVTYTEPGLKGDTATNTLKPATVETGAT
                     VRVPLFINVGDKIKINTTEGSYVERAK"
     gene            complement(818..>1490)
                     /locus_tag="PKN90_06495"
     CDS             complement(818..>1490)
                     /locus_tag="PKN90_06495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ85959.1"
                     /translation="FNKKHRPVIFSPHPIYDLFGKGTDKKLALLQLGLNPNYQYVLFF
                     GAIKESKGLDLLIEAFADERVRQLPIKLLVVGDFNNNKEFYTDIMYENRLKNNIIFVD
                     EYVPNHVVSLYFSACSIVGQPYREVNQSGILQVAYHFDKPILITNIAQCGINIPNQKV
                     GYVINPNTTEIADSLVDFFVNKREEEMVNNVKIEKKKFMWNNMTEAIYSLYQTAKAYK
                     NTRLI"
BASE COUNT          452 a          243 c          239 g          556 t
ORIGIN      
        1 aagtgcgaag aagcaggtgt catcatacaa tatgctggat attagtgaat acaatcaggt
       61 attatagttg tttgaagtat atttactaca tatttcttgt tactttcatt atactattaa
      121 aagaggctgg ctcattttat tttgagccag cctcttttaa tatctcatag ggaaagttat
      181 tttgcacgtt ctacatacga tccttctgtt gtatttatct taattttatc cccaacattg
      241 ataaacaaag gaacacgaac tgtagcacct gtttctactg tagcaggttt tagtgtgttg
      301 gttgcagtat ctccttttaa tcctggttca gtgtatgtta cttctaggat ggtggtggta
      361 gcaaggtcgg caaataatat tgtttctgta tcagcatgta cagttacttc tgcaatatct
      421 ccttctttca agaagtccac tccattgatt ttgtctttat caattgatat ttgttcaaat
      481 gtttcgttgt gcataaagtt gtatcccata tcatctttgt ataaaaattg atatggacga
      541 cgttcgatac gtacttcatc aagttttacg cctgcattaa atgtattttc gagaatacga
      601 cctgtttgaa catttttcat ttttgtgcgt acaaatgctg cacctttacc tggttttacg
      661 tgtaaaaatt ctacaattgt gtaaagtcca ttgttaaatt caatacacat accatttctt
      721 atgtctgctg tagttgccat tggaataatt taagtatata atttgctaat aattagggtg
      781 caaaaatagt tatttgttct ataacatcaa aaaaaaatta tatcaaacga gtatttttat
      841 aagccttagc tgtttgatat aaactgtaaa tggcttcagt catattgttc cacataaatt
      901 tctttttctc tatttttaca ttgtttacca tttcttcttc gcgtttattg acaaaaaaat
      961 ctactaacga atccgctatt tctgtagtat ttggattaat cacatagccc actttttgat
     1021 taggaatatt aatgccacat tgagcaatat ttgtaattag aatgggtttg tcaaagtggt
     1081 aggctacttg taaaattccg ctttgattta cttctctata gggttgtccg acaatgctac
     1141 atgctgagaa atagagacta acaacatgat taggaacata ttcgtctaca aaaatgatat
     1201 tatttttgag tctattttcg tacatgatat cggtataaaa ctccttatta ttgttaaaat
     1261 ctcccactac caaaagcttt ataggtaatt gacgtacccg ttcatctgca aaagcttcaa
     1321 taagtaaatc caacccttta ctttctttga tagctccaaa aaataagaca tattgataat
     1381 ttggatttaa accaagttgc agaagagcta attttttgtc agtgcccttg ccaaaaaggt
     1441 cataaatagg gtgaggagaa aaaatcactg ggcgatgttt tttattaaaa
//
LOCUS       DAOPUR010000377         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_97747_length_1483_cov_3.386555, whole genome shotgun sequence.
ACCESSION   DAOPUR010000377 DAOPUR010000000
VERSION     DAOPUR010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97747_length_1483_cov_3.386555"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKN90_06500"
     CDS             complement(<1..484)
                     /locus_tag="PKN90_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNZ85960.1"
                     /translation="MPHIIIADNQAISRFGIYQLLSKQAISYQSTECTNKEELVIALR
                     EHTQAVIILDYTLFNFLGIDDLLVLAERYPRAHWLLFSDELSPDFLQRALCSATNISV
                     VMKDASQEELQLAIQYSIRKDRFVCNYVSNLLLSAKTIDANYAIQLTDTEKIILKEIA
                     L"
     regulatory      620..718
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            834..>1483
                     /locus_tag="PKN90_06505"
     CDS             834..>1483
                     /locus_tag="PKN90_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNZ85961.1"
                     /translation="MKKVFISFSGLLLSASVAFGQADTLRLIDLQEVQVQSTRASSQT
                     PMTFSTVTKSDLQQQNAGQDIPFLLSLTPSVVATSDAGNGVGYTGIRIRGTDANRINV
                     TANGVPINDSESHGVFWVNMPDFSSSLQDIQIQRGVGTSTNGAAAFGGSLNMKTENLA
                     IVPYAELHGSYGAFNTSKLTVKAGSGLLRDRWAFDARLSTIQSDGFIDRASTDLSS"
BASE COUNT          404 a          272 c          288 g          519 t
ORIGIN      
        1 ccaaagctat ttctttcagt attatttttt cggtatctgt taactgtatg gcgtaattag
       61 catctatcgt tttggcagaa agcaataaat tacttacata attgcataca aaacggtctt
      121 tacgaatgct atattgtatg gctagttgta actcttcttg cgaagcatct ttcattacta
      181 cactaatatt ggttgcacta cataaggctc gttgcaaaaa gtcgggactc aactcatctg
      241 aaaacaataa ccaatgagca cgtggatatc gttcggctaa taccaataaa tcgtcaatgc
      301 ctaaaaaatt aaatagggta taatccaaaa taattaccgc ttgtgtatgc tctctaagtg
      361 caattaccaa ttcctccttg ttcgtacatt cggtagattg gtaggatata gcttgtttgc
      421 ttagcagttg gtagatgcca aaacgactaa tggcttggtt atcggctatg atgatgtgtg
      481 gcatagttgc tatgttttct tataacaaag atacactttt tttttattga ttatactttt
      541 aacaaacctt ctgatatgtt ataaaacata atttttcttt catatttcaa ttattctaaa
      601 aataatgtgt acttttgcat cgatatgtat ggggtgcttg agtttttctc aggctgagat
      661 tatacccttt gaacctgata tggataatgc catcgcaggg aaaaagattc gctttgtgca
      721 caagcttttc ggtattcttc tctttttatt accccttttt tgtttaatct tttttttgaa
      781 atggctcttg gcgtttacgt tatggcattt caaaaagtaa aaaatgtttc taaatgaaaa
      841 aggtatttat ttctttcagc ggacttttat tgtctgcatc tgtagccttt gggcaggcag
      901 atacgcttcg tttaattgat ttacaggaag tacaagtgca atctactcgt gcttcgagtc
      961 aaacaccaat gacattctct acggttacca agtctgactt gcaacaacaa aatgcaggac
     1021 aagatattcc gtttttattg tcgcttacac cctcggtcgt agccacttcg gatgcgggca
     1081 atggggtagg ttatacgggt attcgcatac gtggtacaga tgccaatcgt atcaatgtta
     1141 ctgccaatgg cgtaccgatt aacgattcag aatcgcatgg tgtattttgg gtaaacatgc
     1201 ccgatttttc ctcatcattg caagatattc agattcagcg aggcgttggt acttctacca
     1261 atggtgcagc cgcttttggt ggtagtctga atatgaaaac agagaatctt gccattgttc
     1321 cgtatgctga actgcatgga agttatggtg cgtttaatac ttctaaactt accgtaaaag
     1381 cgggttcggg tttattacgc gaccgttggg cgttcgatgc tcgtttatct accatacaat
     1441 ccgatggttt tattgataga gcatctacgg atttatcttc tta
//
LOCUS       DAOPUR010000378         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_99697_length_1465_cov_2.469504, whole genome shotgun sequence.
ACCESSION   DAOPUR010000378 DAOPUR010000000
VERSION     DAOPUR010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99697_length_1465_cov_2.469504"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1465
                     /locus_tag="PKN90_06510"
     CDS             <1..>1465
                     /locus_tag="PKN90_06510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNZ85962.1"
                     /translation="TEIDSLLANKTTPTFENTIVALEKTGDVISRTSAVFFNLLHAET
                     NDTLENLAQELSPILTEHSNKIYQNQALFERVKAVYAQKETLTLTAEQQQLLENTYQA
                     FVRSGAELSEKDKQTYTELSTKLSQLTLTFSQYVLKETNAYSYVITDEKELDGLPDYA
                     KEAAAEKAKAKNQEGWLIDLSMPSYLPIMQYATNRELRKKLYFAYNTKGNQGNEYDNK
                     QTITDIVNTRLQLAQLMGYSNYATYKLVRTMAQDTSKVYALIDQLYTAYQPVASQEFD
                     EIQAFARTFENNATGELMPWDWAYYAEKVKEQKYQVNDELLKPYFELEKVKQGVFGLA
                     TALFDLQFTKNTSIQVYHPDVDAYEVHDSKGNFIAILYTDFHPRESKKGGAWMTEFKE
                     QWIENGENSRPHISIVMNFTPPTATSPSLLTFNELTTLLHEFGHALHGMLSNTTYASL
                     SGTSVYRDFVELPSQLLENWATEKEFLAYNIDSLQHEF"
BASE COUNT          533 a          327 c          251 g          354 t
ORIGIN      
        1 actgaaatag actctctctt agccaataaa acgactccaa catttgaaaa taccatagta
       61 gcattagaaa aaacaggaga tgtaataagc cgcacaagtg ctgtattttt caacttattg
      121 catgctgaaa caaacgatac cttagaaaat ctggctcaag aactatctcc gatactcact
      181 gaacacagca ataagattta ccaaaatcaa gccctttttg aacgagtaaa agccgtatat
      241 gctcaaaaag agaccttaac tctgacagcc gaacaacaac aattactcga aaatacctac
      301 caagcatttg tacgctcagg ggctgaatta tcagaaaaag acaaacaaac gtacactgaa
      361 ttaagcacca agctaagcca actaacactt acgtttagcc aatatgtact gaaagaaaca
      421 aacgcttatt catacgtaat caccgacgaa aaagaactgg acggattgcc tgattatgcc
      481 aaagaagcag ctgcagaaaa agccaaagcc aaaaatcaag aaggttggct tatcgacctt
      541 tcgatgccaa gttatttgcc cataatgcaa tatgcaacca accgcgagtt acgcaaaaaa
      601 ctatattttg cctacaatac caaagggaat caaggaaatg aatacgacaa caaacagacc
      661 ataacagata ttgttaacac ccgtctgcaa cttgctcaac taatgggtta cagcaactac
      721 gctacctaca agctagtacg taccatggct caagacacca gcaaggtata tgcacttatc
      781 gaccaactat atactgctta tcagccagtt gcctcgcaag aatttgatga aatacaagcc
      841 tttgctcgca cgtttgaaaa caatgctacg ggtgaactca tgccttggga ttgggcatat
      901 tatgccgaaa aagtaaaaga acaaaaatac caagtaaacg acgaattact caaaccctat
      961 tttgaactcg aaaaagtaaa acaaggagtg tttggtctag ctactgccct ttttgattta
     1021 cagtttacaa aaaacacttc tatacaagtg tatcatccag atgtagatgc ctatgaagtg
     1081 catgatagca aggggaattt tattgccata ttatacaccg attttcatcc aagagaaagc
     1141 aaaaaaggag gtgcgtggat gactgagttt aaagaacaat ggattgaaaa cggagaaaat
     1201 agtcgccccc acatatccat tgtaatgaac tttacaccac ccacagctac tagcccgtcg
     1261 ttgctcactt ttaatgaatt aacgaccttg ctacacgaat ttggacacgc tttgcacggt
     1321 atgttatcca acaccaccta cgcaagcctc tctggaacaa gcgtttaccg cgactttgta
     1381 gaactcccct ctcaattgct cgaaaattgg gctacagaaa aagaatttct tgcatataat
     1441 attgattcac tgcaacatga atttt
//
LOCUS       DAOPUR010000379         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_101056_length_1452_cov_3.256263, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000379 DAOPUR010000000
VERSION     DAOPUR010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101056_length_1452_cov_3.256263"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PKN90_06515"
     CDS             <1..1122
                     /locus_tag="PKN90_06515"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNZ85963.1"
                     /translation="ENIKTQVKNAQEYVQSDEFKSNAKNIGSRIERILSPIVKTVFIV
                     VSSIVGFTLLLVSLSLLAAFVIALLHADILLAFLPFSVGSGYLAIIGIVSIFLLIITP
                     TIGLFVGAIRLLSNKTPRKGKSVFGWILFTVWVISLVALISVIVRGTLEYKPSYTRSF
                     AHYPAENRTLDGFHTILLHNGVHAEYTQDSTQQVQIMAPDYTLQNIRTEVNDSILNIY
                     FDGETNSIFTKNFAIHIQSPTIKRIEVEGGSSITTNTAIKTSNLQLKVADLSQAQLLL
                     DVEKNINVISTGLSRVQLSGNTHHLDLNVSNASMANTVDLVSKTGTVNVQNAAKADIQ
                     VRDSVWIQANDAARVCYRGNPIIKQQAYNAAVVVGYNEQ"
BASE COUNT          491 a          279 c          261 g          421 t
ORIGIN      
        1 gaaaacatta aaacgcaagt taaaaatgcc caagagtatg ttcaatcaga cgaatttaaa
       61 agcaatgcca aaaacattgg ctctcgcatt gaaagaatac tctctcctat cgttaaaacg
      121 gtattcatag tcgttagttc tattgttggt tttaccttgc tacttgtatc tttatcgttg
      181 ctagcagcat tcgttatagc attgctacat gcagatatat tacttgcatt tttaccattt
      241 agtgttgggt cggggtattt ggcaattatt ggcatagtga gtattttctt acttattatt
      301 acgccaacta tcggactatt tgtcggagcc attcgcttat tatcaaacaa aacacctcgt
      361 aaaggtaaaa gtgtattcgg ttggattttg tttacagtat gggtcatcag tttagtagca
      421 ctcatctctg taatagtaag aggcacactg gagtataaac cctcctatac aaggtctttt
      481 gctcattacc ctgccgaaaa tcgtacccta gatggatttc ataccatctt attgcacaat
      541 ggcgtacatg ctgagtacac acaagactct acacaacaag tacaaattat ggctcccgat
      601 tacaccttac aaaacatccg tacagaagta aatgatagca tattgaacat ctattttgat
      661 ggagaaacaa attcaatctt tactaaaaat tttgccatac acatacaatc gccaaccata
      721 aaacgcattg aggtagaagg aggctcaagc attacgacaa acacagccat taaaacaagt
      781 aatttgcaac tgaaagtagc tgatctaagt caggctcaac tactgctaga tgtagaaaag
      841 aatataaacg tgattagtac tggcttatcg cgggtacagc tatcgggcaa tacgcatcac
      901 cttgacttaa acgtaagcaa tgccagcatg gcaaacacag ttgatttagt gagcaaaacg
      961 ggcactgtca atgtacaaaa tgcagccaaa gcagatatac aagtacgcga cagtgtgtgg
     1021 atacaagcca acgatgctgc acgtgtatgc tatcgtggca acccaatcat taaacaacag
     1081 gcatacaatg ctgcggttgt agtaggctat aacgaacaat gacatgtatt tgaacacaaa
     1141 agagcggtca aaatttgacc gctctttgtg atttttcata ggtactcgac acaaaatccc
     1201 aggctacaag tatttcgaat gtcagacact acaaagatga tacttttttt tgaattctga
     1261 aatagcgtag tatatgttat acaatatatt ttttagttaa aattacgcat ccactatgcc
     1321 tttttgacag acagacatac ttggcactac ttttgcatag ctaaagtgaa acaaatgtat
     1381 aacttaaaat aaatagttga gtatgaaaaa aggaaatatt ggtgtaacta cggaaaacat
     1441 tttccctatc at
//
LOCUS       DAOPUR010000380         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_101855_length_1445_cov_2.706475, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000380 DAOPUR010000000
VERSION     DAOPUR010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101855_length_1445_cov_2.706475"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>1445
                     /locus_tag="PKN90_06520"
     CDS             12..>1445
                     /locus_tag="PKN90_06520"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNZ85964.1"
                     /translation="MVFYENKVLIKYTMLETQSATTLRLKPLLAFRSVHELCKENSLI
                     NNSYTEIANGISVQLYPTYPTLHIQLSKSNTYEHTPHWNKNIYYAKEAERGFECTEDL
                     YAPGTFEVSLKQGESVIVAVGTESSAIKTLKTLWEKEVSKRNPRTDLYNCLRNSAVQF
                     YTRTQDNWYLLAGYPWFVVRARDQFISLPGCTICIGETEMFEKIMHSSIQAIKQFMEK
                     GTLSDNIQELDSPDSLLWCIWCLQQYGVYVSEEEMIAKYGDFIVDVVEFIRRQKHPNL
                     FVHSKGLLHISGKEAPATWMNAEENNQPITPRTGFVVEINALWYNALKCSETIHEING
                     NSKQSHILHYQAELAGNEFIQTFWNGMYLYDYVDGIERNMEVRPNMIFATSLPYSPLT
                     KQQQKSILDICTKELLTPKGLRSLSPKSGSYRPNYVGGMLERNRNYHNGPVWTWTLGA
                     YTETYLKIHKKSGVSFIERIVVGLEAEM"
BASE COUNT          515 a          261 c          279 g          390 t
ORIGIN      
        1 aagagcgttg tatggtattt tacgaaaata aagtattgat aaaatataca atgcttgaaa
       61 ctcaatcggc aaccacttta cgattaaaac ctctcttagc ttttcgttca gtacatgaac
      121 tgtgtaaaga aaattcgttg ataaacaaca gttacacaga aattgccaat ggtattagtg
      181 tgcaactata ccctacttac ccaacattac atatacaact ttcaaaaagc aacacctacg
      241 aacatactcc gcattggaac aaaaatatct attatgcaaa agaagcagaa cgaggttttg
      301 aatgtacaga agacttatat gctcctggca catttgaggt aagcctaaag caaggagaat
      361 ctgtaattgt agctgtgggt acagaatcaa gtgctataaa aacactgaaa acattgtggg
      421 aaaaagaagt aagtaaacgc aatcctcgta cagatttata taattgttta cgaaactctg
      481 cggtgcaatt ttatacacgc acacaggata actggtattt attagctggc tacccatggt
      541 ttgtagtacg agctcgcgac caatttatat cccttcctgg ttgcactatc tgtataggag
      601 aaacagaaat gtttgaaaaa atcatgcatt catccataca ggctatcaaa caattcatgg
      661 aaaaggggac actatcggac aacattcaag aattggattc accagatagt cttttatggt
      721 gtatttggtg tttacaacaa tatggagtat atgtatccga agaggagatg atagcaaaat
      781 acggcgattt catcgttgat gtagtagagt ttattcgaag acaaaaacat cccaatctat
      841 ttgtgcattc aaaaggatta ttacatatta gcggaaaaga agctcctgcc acatggatga
      901 atgcagaaga aaacaatcag cctattactc cccgaacagg ctttgtcgta gaaattaatg
      961 ctttatggta caatgcttta aaatgttcag aaacaataca tgaaattaac ggaaacagca
     1021 agcaatcgca tattctacat taccaagcag aattggcagg aaatgaattt atacaaacgt
     1081 tttggaacgg catgtatttg tatgattatg ttgatggaat agaaagaaat atggaagtga
     1141 gaccgaatat gatttttgcc acctcgctac cctattctcc tcttaccaaa cagcaacaga
     1201 agtctatttt agatatttgc accaaagaat tgctcacacc taaggggctg cggtcgttaa
     1261 gcccaaaaag cggctcttat cgaccaaatt acgtgggtgg aatgctcgaa cgtaaccgaa
     1321 attaccacaa cggacccgtt tggacatgga cattaggggc atacacagaa acatatctaa
     1381 aaatacataa aaaaagcgga gtgtctttta ttgagcgaat cgttgttgga ctagaggcag
     1441 aaatg
//
LOCUS       DAOPUR010000381         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_101978_length_1444_cov_2.526278, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000381 DAOPUR010000000
VERSION     DAOPUR010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101978_length_1444_cov_2.526278"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1301)
                     /locus_tag="PKN90_06525"
     CDS             complement(45..1301)
                     /locus_tag="PKN90_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ85965.1"
                     /translation="MKALMFGWEFPPQILGGLGTASYGLTKGMAQQTDMEITFVLPKP
                     NGDEDQSFLRLVGANHVPVVYKETSNEYIEQIIGKLTRLDDYLHYRNNIRYDFSRIST
                     NELGCIYFSGRYPDNLVEEISNYEAVASVLAHSLDFDIIHSHDWLTYPAGKFAKQISG
                     KPLVVHVHATDFDRSRGNVNPTVYAIEKAGMDAADHIITVSNLTRQTVIDKYHQPPHK
                     VTTVHNAVEPLEHAETVIKAQRSEKLVTFLGRITMQKGPEYFVEAAHRVLEKTNNVRF
                     VMAGSGDMMHAMIDLVAKRNIADRFHFTGFLKGKQVHQLLADSDVYIMPSVSEPFGIS
                     PLEAMQVGTPSIISKQSGCAEILAHAIKTDYWDIDAMADAIYAIVHYPAMYKSLRNEG
                     KAEVDSITWEKAGLKVRKIYDMVLHQ"
BASE COUNT          383 a          307 c          263 g          491 t
ORIGIN      
        1 tgtagttgaa aatagaaaca gatatttttc atattcattc ttttttattg atgtaaaacc
       61 atatcgtaaa tttttcgaac tttcagtcca gctttttccc acgttatgga gtctacttct
      121 gcttttccct cgtttcgtag gctcttatac atagcaggat agtgtacgat ggcataaata
      181 gcatctgcca tggcatctat atcccaatag tctgttttaa tagcatgtgc taaaatttcg
      241 gcacagcccg attgtttcga aattatactt ggtgttccaa cttgcatggc ttctaatggc
      301 gaaatgccaa aaggctccga cacagatggc ataatatata catcgctgtc agctaataat
      361 tgatgaactt gtttcccttt caaaaatcct gtaaaatgga accggtcggc aatattgcgt
      421 ttggctacta aatcaatcat tgcatgcatc atgtcgccac tgcctgccat tacgaaacgc
      481 acattattgg ttttttccaa taccctatgt gccgcttcta caaaatattc tggtcctttt
      541 tgcatggtga ttcgccccaa aaatgtaact aatttctctg aacgttgtgc ctttatgaca
      601 gtttcggcat gttcgagcgg ttctacggca ttatgcaccg ttgttacttt gtgtggtggt
      661 tgatggtatt tatcaattac tgtttgtcgg gttaagttgc ttaccgtgat aatatgatcc
      721 gcagcatcca ttccagcttt ttctatggca tataccgttg ggtttacatt gccacgactt
      781 ctatcaaaat cagtagcatg tacatgtact actaagggct ttccgctaat ctgtttggca
      841 aatttacctg ctggatacgt aagccaatcg tgcgaatgaa ttatgtcaaa gtcgaggcta
      901 tgggcaagta cgctggctac tgcttcgtag ttgctaattt cttctaccaa attatctgga
      961 taacgcccag aaaagtagat acagcccaat tcgttggttg aaatacgcga aaaatcatac
     1021 cgaatattgt ttctataatg caaataatca tctaaacggg ttagttttcc aattatctgc
     1081 tcaatatact cattcgatgt ttctttgtac actactggta catgattggc tccaacaagg
     1141 cgtaaaaaac tttggtcttc gtcgccattg ggctttggta atacgaacgt tatttccata
     1201 tctgtttgtt gagccatgcc ttttgttaag ccatagctcg ctgtacctaa ccctcccaaa
     1261 atttgtggag gaaattccca gccaaacatc aatgctttca tattcgtata gtttattcgt
     1321 cttgttgaat gttttgcaat aaccgcaata cccttaaaat ttctgctaca ttcatagcaa
     1381 acgacatccc tccatgtcct ttatatggtg gattaccatc gtatagttcc gataaggtgc
     1441 caat
//
LOCUS       DAOPUR010000382         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_102495_length_1439_cov_2.761561, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000382 DAOPUR010000000
VERSION     DAOPUR010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102495_length_1439_cov_2.761561"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..>1439
                     /locus_tag="PKN90_06530"
     CDS             255..>1439
                     /locus_tag="PKN90_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ85966.1"
                     /translation="MEEEIKNRHPEAEYTNDNIKTLEWQEHIRLRPGMYIGKLGDGSS
                     PDDGIYVLLKEVLDNSIDEFTMGNGKQIDVSIANGRVEVRDYGRGVPLESIVDVTSKM
                     NTGAKYDSKSFVKSVGLNGVGLKAVNALSSYFCIQSFRNGDTATAEFERGTIKTKSGV
                     VPNTVKKGNGTLILFEPDNTIFVGFVYQDEYIETLLKNYVYLNTGLTINYNGKKFYSK
                     NGLHDLLSDTITADTLYPIIHLKGEDIEIAFTHSNQYNDEYYSFVNGQHTTQGGTHLS
                     AFKEAITKTIKDFYNKGFESSDIRNGLVAAISVRVIEPVFESQTKTKLGSKDMEPNGV
                     SVYKYINDFIKKEVDNYLHKHAETAEILLRKIIDSEKERKAIAGVTKIARDRAKKANL
                     HNK"
BASE COUNT          515 a          231 c          262 g          431 t
ORIGIN      
        1 acttatactt ttggatgttg gcaatactac tgtaccttgt aacgcagtgg gcggtattaa
       61 caaatatttc attcttttat tcctatgttt tgtttagtat ggatgaaagc gtttatacta
      121 tctttcaaat tgcaaaaata cacattttca agcatatttc tttaacccat tatttttttt
      181 aaaaaagtac atcttttccc atatctaaat ctttcctttt tatttaaaaa aatgtacctt
      241 tgtacgttaa aattatggaa gaagaaatta aaaatagaca tcccgaagct gaatacacca
      301 acgataacat caaaacgttg gaatggcagg agcatatacg cttacgccct ggcatgtata
      361 ttggtaagct aggagatggc tcctcgcctg atgatggtat atatgtgcta ctcaaggagg
      421 tattagacaa ctcgatagac gaatttacta tgggtaatgg gaaacagatt gatgtttcca
      481 tagcaaatgg acgggttgaa gtaagagatt acggacgtgg agttccctta gaaagtattg
      541 ttgatgtaac ctccaaaatg aatacaggag ctaaatacga ctctaagtct tttgttaaat
      601 ctgtggggtt aaacggtgta ggtctaaaag cggtaaatgc attgtcttcg tatttttgca
      661 tacaaagttt tagaaatgga gataccgcaa cggcagaatt tgagcgtggc acaattaaaa
      721 ccaaatcagg agttgtaccc aacaccgtaa aaaagggcaa tggaactctt attctttttg
      781 agccagataa tacaattttt gtaggttttg tataccaaga tgaatacata gaaaccttat
      841 taaaaaacta tgtttattta aacacagggc ttaccatcaa ttataatggt aaaaagtttt
      901 atagtaaaaa tggattgcac gatttattaa gcgataccat tactgctgat acgctttatc
      961 ctattattca cctcaaagga gaagatatcg aaattgcatt tacacatagt aatcaatata
     1021 atgacgagta ttattcgttt gtaaacggac aacataccac acaaggaggc acacaccttt
     1081 cagcttttaa agaagctatc accaaaacga taaaagactt ttataataaa ggctttgaat
     1141 catcagatat acgcaatggc ttagtggcag ctatcagtgt gcgtgtaata gaacctgtgt
     1201 ttgaatcaca aactaaaacc aagttaggtt ctaaagatat ggaaccgaac ggtgtgtcgg
     1261 tatataaata catcaacgat tttataaaga aagaagttga caattatttg cataaacacg
     1321 cagaaactgc tgaaatttta ctcaggaaaa ttattgattc agagaaagaa cgcaaagcaa
     1381 ttgcaggtgt tactaaaata gctagagaca gagcaaaaaa agccaattta cacaacaaa
//
LOCUS       DAOPUR010000383         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_102506_length_1439_cov_2.562139, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000383 DAOPUR010000000
VERSION     DAOPUR010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102506_length_1439_cov_2.562139"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..>1439
                     /locus_tag="PKN90_06535"
     CDS             27..>1439
                     /locus_tag="PKN90_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85967.1"
                     /translation="MIKDTYGCNSEKTKMLTVIDQKQGHLDFGVFDIRPNPMCINSTE
                     AQIIGQRTDITYLGLSVPAMQISQNEGVLHLDPSRGRIGNHSVIAQRIDVNGCVAKQR
                     VPFQIVAPTRVFPFNIAKTYCTYDEDVQLVVNSASPTTGTISIDNITDPLHPVNVLST
                     LDPASGNVYFQPGWGAGKYEIRYEYSDGNCDNIQRDTVDVYAPSVINMNLKSDYCIKE
                     VINLASTPLGGVYSSDAVANALVNNRFYTERAGLGLHKIRYIQTNEHGCVSKDSTTIQ
                     VRGASVLTIADLKDQYCEPKGTVNIYGFPNNPADGSVWFTGPTFLTSLPNGYASLDLS
                     QATYNANYNVTYHYQENYVNAQGTPESCTTTVTKGFRVLNEAADFWGYNHMDVICGTQ
                     DSVVITGNKAPLGRFVFTGPAANFKDRGNGTAVIYPPTLSVGDYQVTYYYDHKNTQGV
                     TICSTEKAKTFKITQIAPIED"
BASE COUNT          472 a          253 c          285 g          429 t
ORIGIN      
        1 cagcaggttc gtattatatt cagtatatga ttaaggatac atatggctgt aattctgaaa
       61 aaacgaagat gctgactgta atcgaccaaa aacaaggtca tctagatttt ggtgtttttg
      121 atattagacc caatcctatg tgtatcaatt ctactgaagc tcaaattatt ggtcagcgaa
      181 cagatatcac gtatttaggc ttatccgtac ctgccatgca aatttctcag aatgaaggcg
      241 ttttacatct tgatccgtca cgtggcagga ttgggaatca tagcgttatt gcacaacgga
      301 tagatgtgaa tggttgtgtt gccaaacagc gagttccatt tcagatagta gcacctacga
      361 gggtatttcc tttcaatatt gctaaaacat attgtaccta cgacgaagat gtgcaattag
      421 tagtaaattc tgcgtcacct actacaggaa ctatttctat cgataatata acagacccat
      481 tacatcctgt gaatgtactc tctacgcttg atccggctag tggaaatgta tatttccaac
      541 cagggtgggg tgccggtaag tacgaaatcc gttacgaata ttctgatggt aattgtgata
      601 atatacaaag agatactgta gatgtatatg ctccttcggt aattaatatg aatttgaaat
      661 cggattactg tatcaaagag gtaattaatt tagcatcaac tccattaggt ggtgtttatt
      721 catctgatgc ggttgcaaat gctcttgtta acaatcgttt ttatacagaa agagcaggat
      781 tgggtttaca taaaataagg tatattcaaa ccaatgagca tggatgtgta tctaaagata
      841 gtactacaat acaagtaagg ggtgctagtg ttttgactat tgccgattta aaagaccaat
      901 attgtgaacc caaaggaact gtcaatattt atggtttccc aaataaccca gctgatggta
      961 gcgtttggtt tactggacca acatttctaa caagcttgcc aaacggatac gcaagtttag
     1021 atttatcaca agctacctat aatgcgaatt acaacgttac ataccactat caagagaatt
     1081 atgtaaatgc acaaggtacg ccagaatcat gtacaactac tgtaaccaaa ggttttaggg
     1141 tgttaaatga agcagccgat ttttggggat ataaccacat ggatgtaata tgtggtacac
     1201 aagattctgt ggtaataaca ggtaataaag ccccacttgg caggtttgta tttactggac
     1261 cagcagccaa ttttaaagat agaggaaacg gaacagccgt gatttaccct ccaaccttgt
     1321 ctgtagggga ttatcaagtt acctattatt atgaccataa aaatacgcaa ggtgtaacta
     1381 tttgctctac agaaaaagcc aaaacattta aaataactca aatagcacct atcgaagat
//
LOCUS       DAOPUR010000384         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_103044_length_1434_cov_2.625091, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000384 DAOPUR010000000
VERSION     DAOPUR010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103044_length_1434_cov_2.625091"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1381
                     /locus_tag="PKN90_06540"
     CDS             56..1381
                     /locus_tag="PKN90_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNZ85968.1"
                     /translation="MSALARYFKQLGHQVGGYDATENTLTQKLESEGMEIHYNDSVNT
                     IPVKYMDKENTTIVYTPAIPHDMSERVFFEKAGFTMLKRAEILGEITRSKKALCVAGT
                     HGKTTTSTILAHILTQSSLGTNAFLGGISKNYSTNLLVNASSDIVVVEADEYDRSFHQ
                     LSPHMAIITATDNDHLDIYQDRENLLSSFQHFTSLIQENGCLLLKKGIELVPSTKNSV
                     KIHTYSANEVADFYATNILIENEKILFDYVTPTFTIEKIKLGVPILINVENAVAAMTI
                     AYLNGVTKEELQHAIETFKGIERRFDFQLQTENVRFIDDYAHHPEELKASIQSIRKLY
                     PNKSICGIFQPHLYTRTRDFATEFANSLSLLDSVILLDIYPARELPIKGVSSKMIFKH
                     IQCKNKSICSKEDLLEEIKKYNFDVLITLGAGDIDKMVPLITQQLKKQL"
BASE COUNT          530 a          235 c          243 g          426 t
ORIGIN      
        1 aaaaataaaa tcgtgccaac aaaattatta ttttttaggt attgggggta ttggtatgag
       61 tgctttagct cgctatttca aacaacttgg gcatcaggta ggagggtacg atgctactga
      121 aaatacactt acacaaaaat tagagtcaga agggatggaa attcattaca acgatagtgt
      181 aaataccatt cctgtgaaat atatggataa ggaaaatacg actatcgtat atacacctgc
      241 aattccacac gacatgagcg aacgagtttt ctttgaaaaa gctggtttca caatgctaaa
      301 aagagcagaa atattaggtg aaattacacg ttcaaaaaaa gccttatgtg tagctggaac
      361 acacggaaaa acgactactt caactatcct tgcccatatt ctcacacaat caagcctcgg
      421 aaccaatgct tttttaggag gtatttcaaa aaattacagc acaaatttat tggtgaatgc
      481 ctcatcagat attgtagtag tggaagcaga tgagtatgac agatcattcc atcagttatc
      541 tccacatatg gctattatta cagccaccga taatgaccat ttagatatat atcaagatag
      601 agaaaaccta ctatctagtt ttcaacactt tacatcactt atacaagaaa atggatgctt
      661 gttgcttaaa aaaggtattg agttagtgcc tagcacaaag aattcggtaa agatacacac
      721 ctattcagca aatgaagtag cagattttta tgctacaaac attctaattg aaaatgagaa
      781 aattcttttt gattacgtta cgcctacttt tacaatagaa aaaatcaaac taggagttcc
      841 tattttaatc aacgtagaaa atgcagtagc tgctatgacc atagcatatc taaacggtgt
      901 tactaaagaa gaattacaac atgctattga aacatttaaa ggaatagaaa gacgctttga
      961 ttttcaatta caaacagaaa atgtacgttt tattgacgat tatgcccatc atcccgaaga
     1021 attaaaagcc tctatacaat caatacgtaa actgtatcca aataagtcaa tttgtggtat
     1081 ttttcaaccg catttgtata ccagaacacg tgattttgca actgaatttg cgaatagcct
     1141 ttcgctcctt gacagtgtta ttttattgga tatttatcct gctcgtgagc tacctatcaa
     1201 aggagtaagt tcaaaaatga tattcaaaca catacaatgc aaaaataagt caatttgtag
     1261 caaagaagat ttgctagagg agataaaaaa gtataatttt gacgtcttaa ttacattggg
     1321 agctggagat atagataaaa tggtgccact aattactcaa caacttaaaa aacagcttta
     1381 attgatataa aaatgattaa aaagattgct cttatagtag tttcggtagt gttt
//
LOCUS       DAOPUR010000385         1427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_103824_length_1427_cov_2.670554, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000385 DAOPUR010000000
VERSION     DAOPUR010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103824_length_1427_cov_2.670554"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PKN90_06545"
     CDS             <1..1371
                     /locus_tag="PKN90_06545"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNZ85969.1"
                     /translation="HNVISGIFDGVKKLHKDSKVYGFLGGPGGLVDHKYIELTSEIID
                     EYRNTGGFDIIGSGRTKLEETAQYDKGVEICNKLGIQALIVIGGDDSNTNACVLAEYY
                     LQKNTGIQVIGCPKTIDGDLKNDMIETSFGFDTACKVYSELIGNIMRDANSAKKYWHF
                     IKLMGRSASHIGLECALQTQPNICIISEEVEAKKQTLKEVVDSIAKVVATRAAKGNNF
                     GIALIPEGLIEFIPEMKVLISELNDLLAHESDVQKALNGLSKASAEVFCSLPENIAKQ
                     LTLDRDPHGNVQVSLIETEKLLIEMVGKRLKEMKAEGHYTGKFAGLGHFFGYEGRCAA
                     PSNFDADYCYSLGYNAAQLIATGKTGYMSSIRNTTAPAEQWIAGGVPVTMMMNMERRH
                     GAMKPVIQKALVRLDGAPFKTFAANRDKWATDYCYVYPGPIQYFGPTEVCDQPTKTLQ
                     LEQTGK"
BASE COUNT          480 a          232 c          300 g          415 t
ORIGIN      
        1 cacaatgtaa tttctggtat ttttgatggc gtaaaaaaat tgcataaaga ctctaaagtg
       61 tatggatttt tgggtggacc tggtggttta gttgaccata aatacattga acttacaagc
      121 gagattattg atgaataccg caatactgga ggttttgata ttattggttc tggtcgcaca
      181 aaattagaag aaactgcaca atacgataaa ggcgtagaaa tttgcaacaa attaggcatt
      241 caagcactta ttgttattgg tggagatgat tcaaacacaa atgcgtgtgt attggcagaa
      301 tattatttgc aaaaaaatac tggtattcaa gtaattggtt gcccaaaaac cattgatggt
      361 gacttgaaaa atgatatgat agaaacatca tttggatttg atactgcttg taaagtttat
      421 tcagaactta tcggcaatat catgcgtgat gcgaattctg caaagaaata ctggcatttt
      481 attaaattaa tgggacgttc agcttcgcat atcggacttg aatgtgcttt acagacacaa
      541 ccaaacattt gcatcatttc ggaagaagta gaagcaaaaa aacaaacgct aaaagaagtg
      601 gttgatagca ttgctaaggt ggtagctact cgtgctgcga aaggtaataa ttttggtatt
      661 gcacttattc ctgaagggtt gatagagttt attcctgaaa tgaaagtttt aatttcggaa
      721 ttaaacgact tgctagcaca cgaatcggat gttcaaaaag cactcaacgg attaagcaaa
      781 gcaagtgctg aagtattctg tagcttacca gaaaatattg ctaagcaatt aacccttgat
      841 cgcgacccac atggtaacgt gcaagtatcg ttgattgaaa cagaaaaatt gttgattgaa
      901 atggtaggta aacgattgaa agagatgaaa gccgaaggac actacacagg taaatttgct
      961 ggcttaggac atttctttgg ttacgaagga cgttgtgcag ctccatcaaa ctttgatgct
     1021 gactactgct attcattagg atacaatgca gctcaattga ttgcaacagg caaaacaggc
     1081 tatatgtcgt ctatccgcaa tactactgcc cctgcagaac aatggattgc tggtggtgta
     1141 cctgttacta tgatgatgaa tatggaacgt cgtcatggtg caatgaaacc tgtaattcaa
     1201 aaggcattgg ttcgtttaga cggtgctcca tttaaaacat ttgcagccaa tcgcgacaaa
     1261 tgggctactg actattgcta tgtatatcct ggtccaatac aatattttgg tccaacagaa
     1321 gtatgcgacc aacctacaaa aactttgcaa ttggaacaaa caggtaaata aattacttca
     1381 ttgaagaata aaagcaactc gaacaaaaag ggttgctttt ttagttc
//
LOCUS       DAOPUR010000386         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_104033_length_1425_cov_2.996350, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000386 DAOPUR010000000
VERSION     DAOPUR010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104033_length_1425_cov_2.996350"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..915
                     /locus_tag="PKN90_06550"
     CDS             10..915
                     /locus_tag="PKN90_06550"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HNZ85970.1"
                     /translation="MNKIKVAIVGYGNIGRFSLEAVQAAPDMELMGVVRRASSVGNVP
                     NELNGIKIVSNIDELGKVDVAILCGPTRSIPETAVELVKKGIRTVDSFDIHGEELWKL
                     RQSLDVAGKKTNAASIISAGWDPGSDSVIRTLLLAMAPKGITYTNFGPGMSMGHSVVA
                     RSKEGVKNALSMTIPTGTGVHRRMVYVELENGAKLEKVTEAIKTDSYFSKDETHVMEV
                     ASVDNLQDMGHGVLIERKGVSGKTQNQLFQFSMQINNPALTAQILISCARAVTKQAPG
                     AYTMPEIAPLDYLYGDKETLIKTLV"
     gene            1076..>1425
                     /locus_tag="PKN90_06555"
     CDS             1076..>1425
                     /locus_tag="PKN90_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85971.1"
                     /translation="MKTYTHFLIYLLLFCSFQLGYSQDTNNKQVYRVTLNDKSSYIGE
                     ITEETTSTLVLINQTTGVKTELPTTQIQKKELLSESSIKNNEYWFENPNATRYLFGTS
                     AFNLKKGEGYYQNT"
BASE COUNT          494 a          243 c          266 g          422 t
ORIGIN      
        1 aactaagttg tgaacaaaat aaaagttgcc attgtaggct acggaaacat tggtcgtttc
       61 tcattagaag ctgtacaagc ggctcctgat atggaattaa tgggcgtagt aagacgtgct
      121 tcatcggtag gaaatgtacc aaacgaacta aatggtatta aaattgtatc gaatattgat
      181 gaattaggca aggtagatgt tgctatttta tgtggtccta cccgaagcat tcctgaaaca
      241 gcggtagaat tagtaaaaaa aggcattcgt acagtagata gctttgacat tcacggagag
      301 gaactttgga aactacgcca atcgcttgat gttgctggaa aaaaaacaaa tgcagcctct
      361 attatttcag ctggctggga tccaggtagc gactcagtaa tccgaacact attgttagct
      421 atggctccaa aaggtattac ctataccaac tttggtccag gtatgagtat gggacactct
      481 gtagtagcac gttccaaaga aggagtaaaa aatgctctat caatgactat ccctacgggt
      541 acaggcgttc atcgccgtat ggtgtatgtg gaattagaaa atggtgctaa actagaaaaa
      601 gttacagaag ctattaaaac tgactcgtat ttcagtaaag atgaaactca cgttatggaa
      661 gttgctagtg ttgataattt gcaagacatg ggtcatggcg ttttaattga acgcaaaggg
      721 gtttcgggta aaacccaaaa ccaattgttc caatttagca tgcaaattaa taatccagcg
      781 ttaactgctc aaatattaat ttcttgtgct cgtgcggtta caaaacaagc tcctggtgct
      841 tatacaatgc ctgagattgc tcctcttgat tatttgtatg gagataaaga aactttaatt
      901 aaaaccttgg tgtagaaaac acgtactatt tcatacaagc aagaagcgaa ctcttaacag
      961 ggtatcgctt cttttgtttt tttataaata tcaataaaat attttcgttt tatatgaaat
     1021 actactatat ttgctttagt atttatgtaa atccaatctt taataataaa acattatgaa
     1081 aacgtatacg cattttctta tctatttatt gttattctgt tcttttcagt taggatattc
     1141 gcaagataca aacaacaaac aagtatatcg agtaacctta aacgacaaat caagctacat
     1201 tggcgaaatt actgaagaaa caacctctac tcttgttctc attaaccaaa caacgggagt
     1261 aaaaacagaa ttaccaacca cacagattca aaaaaaagag ctactatcgg agagttctat
     1321 caaaaacaac gaatattggt ttgaaaatcc aaatgcgaca cgttatctat ttggtacatc
     1381 ggcatttaac ttaaaaaaag gcgaaggtta ttatcaaaat accat
//
LOCUS       DAOPUR010000387         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_104888_length_1418_cov_2.392517, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000387 DAOPUR010000000
VERSION     DAOPUR010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104888_length_1418_cov_2.392517"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..>1418
                     /locus_tag="PKN90_06560"
     CDS             458..>1418
                     /locus_tag="PKN90_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNZ85972.1"
                     /translation="MNSNNIQSDNKNLKFKVQLLIAIASSLIFIGKIIAYFLTNSVGI
                     LTDALESTVNVTTGFITLYAVYFSLKPKDNNHPFGHGKAEFLSASVEGFLIMIAGIII
                     IIEAIKRLFVPAEVSQLDTGIVIVAIAGLLNYLLGWYSIRIGKKNNSIALVSGGKHLQ
                     SDTYSSIGLVAGLVLLYYTKLVWLDSLIALIFGAIIIITGLKILKETTSHLMDEADFK
                     LIEKFGKLIDTNKYDEWIDIHNFKLVKYGDVFHINCDLVLPWNMYLSEAHAEGEKLKK
                     ILMSNFNEDIVFHLHTDECFKEYCRHCRRAHCNERTNAFESQLY"
BASE COUNT          486 a          208 c          244 g          480 t
ORIGIN      
        1 atagtttcgg ttgacaaaca ccaacagcta acacacgcct gttttgtcct gaaataggtt
       61 tacacaaaaa agcaaggtat ttgaggatta cggagacaac tatctatttc gctccgctag
      121 agctaactaa caaatcggta tgtattttct attatgatgt agctcttatg gggtatgaga
      181 aatagcattt ttccttaaaa atttgtaaaa cccaagctcc gtaggagcga aatctatgct
      241 agagtcatta tattttttac tgcaatcttc cattcctaca atataagcaa aacaactagc
      301 cctagctgtc gcacaatttt attgattggc tcgcttgata atctcgtacg aatattttga
      361 gtttctctca aaatacatta catttgaccc aacattatac tagtatagct aataataatt
      421 tgtagtgcga gggtttgaca ctaaaaaata acacaaagtg aacagtaata atatacagtc
      481 agacaataaa aatttgaaat ttaaggtaca attacttatt gccattgcct cttctttaat
      541 ttttataggc aaaataattg cctatttttt aactaattca gtaggtatat taactgatgc
      601 cttagaaagt acagtaaatg taaccactgg attcattacc ttatatgctg tttatttttc
      661 tctaaaacca aaagataata atcacccttt tgggcatggg aaagcagagt ttttatctgc
      721 atcagttgaa ggttttttaa taatgattgc tggaataatc attattattg aagccattaa
      781 acgacttttt gttccagcag aagtgtcgca attggacact ggtattgtca ttgttgctat
      841 tgcagggttg ttaaattact tacttggttg gtatagcata agaattggga aaaaaaataa
      901 ttcaattgca ttagtttcag gtggcaaaca cttacaatca gatacgtatt catcaatagg
      961 tttagtggca ggtttagtgt tgctatacta tacaaaatta gtatggttag atagtttgat
     1021 tgctctcata tttggtgcaa taattataat aacaggctta aaaatattga aagaaaccac
     1081 ttcacactta atggacgagg ctgattttaa gttaatagaa aaatttggca agttgattga
     1141 taccaataag tatgacgaat ggattgatat tcataatttt aaacttgtaa aatacgggga
     1201 tgtatttcat attaattgtg atttagtatt gccttggaat atgtatttat cagaggctca
     1261 cgcagaagga gaaaaattaa aaaaaatact catgtcaaat tttaatgaag atatagtttt
     1321 ccatttgcat actgatgaat gttttaagga atactgtagg cattgtagaa gagcacactg
     1381 taatgaaaga accaatgctt tcgaatcgca gttatatt
//
LOCUS       DAOPUR010000388         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_104980_length_1417_cov_3.493392, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000388 DAOPUR010000000
VERSION     DAOPUR010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104980_length_1417_cov_3.493392"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKN90_06565"
     CDS             complement(<1..539)
                     /locus_tag="PKN90_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNZ85973.1"
                     /translation="MLVKTFGAAVHGIDASIITIEVNVSQGIKFFLVGLPDNAVKESH
                     ERIVSALQYNGHKFPRQQIIINMAPADIRKEGSAYDLPLAMGIMAAAEQIACEKINDY
                     LIMGELSLDGNIMPIKGALPIAIKAREAGFKGLILPKQNAKEAAVVNNLEVLGVENIQ
                     EVIGFLNNDTTIEPTIVHT"
     gene            complement(592..1260)
                     /locus_tag="PKN90_06570"
     CDS             complement(592..1260)
                     /locus_tag="PKN90_06570"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNZ85974.1"
                     /translation="MIKIHKEGRMLLLVLFLALTIVNTLLFFIFDNAIVPTIALVISI
                     GFFIFFTHFFRSPERIVLADEHYVVAPADGKIVVIEPVEEHEYLKEKRLQVSIFMSPF
                     DVHANWYPIAGKVIHVAHHDGRKMAAYLPKSSTENERSTVVIETQHKQLVLMRQIAGA
                     LARRIVTYAKVGDNCKINQQFGFIKFGSRVDLYLPLDSEILVDLNEEVVGNETIIAKL
                     KHEV"
     gene            complement(1270..>1417)
                     /locus_tag="PKN90_06575"
     CDS             complement(1270..>1417)
                     /locus_tag="PKN90_06575"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNZ85975.1"
                     /translation="GDLTESLLKRTVDMKDSGNILPGHGGILDRFDSLLLLAPIIYFF
                     LQLI"
BASE COUNT          417 a          281 c          257 g          462 t
ORIGIN      
        1 ctagtatgaa caatggtcgg ctctatcgtt gtatcattat ttaaaaaccc aattacttct
       61 tgaatatttt ctacacctag cacttcgagg ttgttcacaa cagcagcttc tttggcattt
      121 tgtttgggca gaatcaatcc tttaaatcca gcttctcgtg ctttaatggc aatgggtaag
      181 gctcctttga tgggcataat attgccatca agagataatt ctcccatgat caaataatcg
      241 tttattttct cgcaggctat ttgctcggct gccgccataa tacccatagc caaaggtaaa
      301 tcgtatgccg aaccttcttt gcggatatcg gcaggagcca tgttgatgat gatttgttgg
      361 cgagggaatt tatgcccgtt gtattgcaag gcggatacaa tccgttcgtg gctttctttt
      421 acggcattat cgggtagtcc taccaaaaag aactttattc cttgcgatac gttcacttca
      481 attgtaatga tggaagcatc tataccatgc actgcggcac caaatgtttt tactaacatg
      541 agaatatttt agggtcaaac ctcgactaat taagtctgtg tttagagtag attatacttc
      601 gtgttttagt ttagctatga tggtttcgtt tcctacaacc tcttcgttta agtcaaccaa
      661 tatttctgaa tcaagtggta aatacaaatc taccctcgaa ccaaatttga taaatccaaa
      721 ctgttgattt atcttgcaat tgtcgcctac tttcgcataa gttacaatgc gtctggctaa
      781 agctcctgct atttgcctca tgagtacaag ttgcttatgt tgtgtttcaa tcactactgt
      841 cgaacgttca ttttctgtgc tcgattttgg taaataggca gccatcttac gtccatcatg
      901 atgggctaca tggataactt tacctgctat tggataccag ttggcatgta catcaaaagg
      961 agacatgaaa attgatactt gcaaacgttt ttcttttaaa tattcatgct cttctacggg
     1021 ctcaataacc acaattttgc catcggctgg tgccaccaca taatgttcgt ctgctaacac
     1081 aatgcgttca ggactgcgaa agaaatgcgt aaagaaaata aaaaaaccta ttgaaataac
     1141 aagggcaatt gtaggtacta tagcattgtc aaaaatgaaa aacaacagcg tattaacaat
     1201 cgttagggct aaaaaaagaa caaggagcaa cattcgtcct tctttgtgga tttttatcat
     1261 agtatttttt taaattagtt gtaagaaaaa atatataata ggagccaata ataacaaact
     1321 atcaaaacga tcaagtattc ctccatgtcc aggaagtatg ttcccagaat ctttcatgtc
     1381 tactgttctt tttaataggg actctgttaa atcgccc
//
LOCUS       DAOPUR010000389         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_105099_length_1416_cov_3.323292, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000389 DAOPUR010000000
VERSION     DAOPUR010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105099_length_1416_cov_3.323292"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKN90_06580"
     CDS             <1..393
                     /locus_tag="PKN90_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNZ85976.1"
                     /translation="EQSIHAMNQSCSNKHYSIIETFFDGYKKEVIKHLEYEDSSVFPY
                     VYKLIAGEKTPGFGIQQFEKNHTNIEEKLGDLKNIIIKYVPNDCPSRERNKLLYDIFL
                     FEEDLNRHTLIEDKVLIPYVESIEKSQN"
     gene            417..1034
                     /locus_tag="PKN90_06585"
     CDS             417..1034
                     /locus_tag="PKN90_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNZ85977.1"
                     /translation="MITQTNQRIIIIEPSTIVFAGLQSIIERMHIAESIYHVTELDTI
                     TQKLAQLRADIIIVNTAVFGFHKNLSIKSIFKDCTHASIIAFVSQYSDSQFLKQFDDI
                     IDIYDDAQTISAKIRHIIEQDTIDITETDTTELSEREQEVLIAVVKGLINKEIASTLN
                     ISIHTVMSHRKNITRKTGIKSISGLTVYALFNNLIKQEDVQQPHH"
     gene            1127..>1416
                     /locus_tag="PKN90_06590"
     CDS             1127..>1416
                     /locus_tag="PKN90_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNZ85978.1"
                     /translation="MRLSDLQTGEKAIIVKVLGHGNFRKRIIEMGFIKGKKVEVLLNA
                     PLKDPIKYKVMDYEVSLRRNEAELIRVISEKEALASSTTSNYFGTIEASSLDA"
BASE COUNT          532 a          277 c          218 g          389 t
ORIGIN      
        1 gaacaatcta tacatgcaat gaaccaatcc tgctccaata agcattatag tattatcgaa
       61 actttttttg atggatacaa aaaagaagta atcaaacacc tcgaatacga ggatagtagc
      121 gtttttcctt atgtgtataa actgattgca ggagaaaaaa caccaggttt tggtatccaa
      181 caatttgaaa aaaaccacac caatattgaa gagaaactag gggacttaaa aaacattatc
      241 attaaatatg tgcccaacga ttgcccttcg cgtgaacgca ataaattact atacgatatt
      301 ttcttattcg aagaagacct caaccgacat acattgatag aagataaagt gttaatccca
      361 tatgttgaaa gcatagaaaa aagtcaaaac taaatgagta gttactacat gccattatga
      421 taacacaaac aaaccaacgc attatcatta tcgaaccttc tactattgtg tttgctggat
      481 tgcaatcaat tatagaacga atgcacatag cggagtcaat ctaccacgtt acagaattag
      541 acacaattac tcaaaaacta gctcaactgc gtgcagatat tattattgtg aatacagctg
      601 tgtttggatt tcataaaaac ttatctatca aatctatttt taaagattgt actcatgcat
      661 ctatcattgc ttttgtcagt caatattccg acagtcaatt tctaaaacaa ttcgatgata
      721 tcatcgatat ttatgatgat gcacaaacaa tctctgcaaa aattagacac atcatcgaac
      781 aagatacaat tgacataacc gaaacagaca ccactgaatt atccgaacgc gaacaagaag
      841 tccttattgc agttgtaaaa ggcttaataa acaaagaaat agcctcaact ctcaatattt
      901 ctatccacac ggtaatgtcg caccgtaaaa atattacccg caaaacaggg attaaatcca
      961 tatctggact tactgtatat gccctattca acaacctaat taagcaagaa gacgtacaac
     1021 agcctcatca ctagttatag tgagtcaaca ggctattttt cactacttat agtcattgca
     1081 aagcaaaaat aaaatactta cctttgcaag ctaaaaacta caatcaatgc gtctatccga
     1141 tttgcaaaca ggagaaaaag ccattatagt aaaggttcta ggacatggca attttcgcaa
     1201 acgcattatc gaaatgggct ttatcaaggg caaaaaagta gaagtactgc tcaatgctcc
     1261 tttaaaagac cccattaaat acaaggtaat ggattacgag gtatctctac gtcgtaacga
     1321 agctgaatta atacgtgtta tcagcgaaaa ggaagcctta gcgagctcta ctacatccaa
     1381 ctattttggt acaatagaag cttcttcgtt agacgc
//
LOCUS       DAOPUR010000390         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_105720_length_1411_cov_2.821534, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000390 DAOPUR010000000
VERSION     DAOPUR010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105720_length_1411_cov_2.821534"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1411)
                     /gene="dnaK"
                     /locus_tag="PKN90_06595"
     CDS             complement(<1..>1411)
                     /gene="dnaK"
                     /locus_tag="PKN90_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNZ85979.1"
                     /translation="GTTNSCVSVMEGNEPVIIANSEGKRTTPSIVAFIDGGERKVGDP
                     AKRQAITNPQKTIFSIKRFMGETYAEVSKEIGRMPYKVVKGDNNTPRVDIDGRLYTPQ
                     EISAMVLQKMKKTAEDYLGSEVTDAVITVPAYFSDSQRQATKEAGEIAGLNVKRIVNE
                     PTAAALAYGLDKSSKDMKIAVFDLGGGTFDISILELGDGVFEVKSTNGDTHLGGDDFD
                     QVIIDWLAEEFTREEGIDLRKDPMALQRLKEAAEKAKIELSSSASTEINLPYIMPVNG
                     IPKHLVKTLSRAKFEQLADKLIQATVDPCQKAMKDAGLSASDIDEVILVGGSTRIPAI
                     QDKVQQIFGKTPSKGVNPDEVVALGAAIQGGVLTGEVKDILLLDVTPLSLGIETMGGV
                     MTKLIESNTTIPTKKSETFTTAADNQPSVQIHVLQGERPMASQNKSIGQFNLDGIPAA
                     QRGVPQIEVTFDIDANGILK"
BASE COUNT          362 a          298 c          257 g          494 t
ORIGIN      
        1 ttttaaaata ccatttgcgt cgatatcaaa agtaacttca atttgtggta cgccacgttg
       61 agctgctggt atgccatcaa gattaaactg tccgatagat ttattttgcg aagccattgg
      121 tcgttcgcct tgaagcacat ggatttgtac agaaggttgg ttgtcagcag ctgttgtaaa
      181 tgtttctgat ttcttggttg gaattgttgt gttcgactca atcaattttg tcattacacc
      241 acccatggtt tcgataccca atgataatgg ggtaacatcc aataatagaa tatcttttac
      301 ttcgccagtt aatacacctc cttgaatagc cgcacccaat gctactactt catctggatt
      361 tacaccttta cttggtgttt tgccaaaaat ttgttgtact ttatcttgaa tagcaggtat
      421 acgagtagaa ccacctacta aaataacttc atcaatatca gacgcgctca atccagcatc
      481 tttcattgct ttttggcaag gatctacagt agcttgaatt aatttatcgg ctagttgttc
      541 aaattttgcc ctgctcaatg ttttaactaa gtgttttgga ataccattta caggcataat
      601 gtatggcaaa ttgatttctg ttgatgctga gcttgataat tcaattttag ctttctctgc
      661 ggcttctttt aagcgttgta atgccatagg atctttacgt aaatcgatgc cttcctcacg
      721 cgtgaattct tcagccaacc agtcgataat tacttggtca aaatcatctc cacccaagtg
      781 tgtatctccg ttagtagatt ttacttcgaa tacaccatcg cccaattcaa gaatagaaat
      841 atcaaatgta ccgcccccta agtcgaatac tgcaattttc atgtccttgc ttgatttgtc
      901 caatccataa gctaaggctg ctgctgttgg ttcgttgaca atacgtttta cgtttaagcc
      961 tgcaatttca ccagcttctt ttgtagcttg acgctgtgaa tcgctaaagt aggcaggtac
     1021 cgtaattaca gcgtctgtaa cttcagagcc aagataatct tcggcagttt ttttcatttt
     1081 ttgcaatacc attgccgaaa tttcttgtgg cgtatacagt ctgccatcaa tatctacacg
     1141 gggcgtgtta ttgtctcctt ttactacttt atagggcata cgtccaattt ctttgcttac
     1201 ttcagcgtat gtttcaccca taaaacgttt aatcgaaaag attgtttttt gagggtttgt
     1261 aatagcttga cgtttagcgg gatcacccac tttacgttcg cctccatcga taaatgctac
     1321 aatcgaagga gtggtacgtt ttccttcgct atttgcaatt ataactggtt cgtttccttc
     1381 cattacagat acgcacgagt tggttgtacc t
//
LOCUS       DAOPUR010000391         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_106053_length_1408_cov_3.532890, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000391 DAOPUR010000000
VERSION     DAOPUR010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106053_length_1408_cov_3.532890"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKN90_06600"
     CDS             <1..349
                     /locus_tag="PKN90_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ85980.1"
                     /translation="DEPSQLSYDNATYYQPNMYRYSDHDPIVVGLKLTPQAPFEPLDK
                     IQLFHDLTSKHIIVTRAKNHQVSIIGIDGRFAHFHSIESDTETINTNMLPAGVYVVKI
                     TGTKEETFKFIQL"
     gene            complement(359..736)
                     /locus_tag="PKN90_06605"
     CDS             complement(359..736)
                     /locus_tag="PKN90_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNZ85981.1"
                     /translation="MKRIISTTKAPAAIGPYSQAIEVNDTLYISGQIPVNPENGKVDA
                     LTIEQQAEQVLQNIGAILQAAGYGYSDVVKSTIFITDMKNFSVVNEVYKKYYTEKFPA
                     RSTVAVKELPLGVMVEIETIAVK"
     gene            complement(745..>1408)
                     /locus_tag="PKN90_06610"
     CDS             complement(745..>1408)
                     /locus_tag="PKN90_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ85982.1"
                     /translation="GGRVWNADELMRMAKLCIQYNVLMVSDEIHADLMLWGKQHIPLA
                     KLSPEIAQHTITFMAPSKTFNIPGLASAFFVAENPQLFAKMQQETANFHLNMGNVFGC
                     MATEVAYNEGKEWLNQAIHYIEQNILFVRNYIEQHIPIIQVVVPDASFLVWLDCRKMG
                     LTSKELKEFMVHKAKVIMNDGATFGQGGEGFQRLNVACPRSVLQQALQQIETAINEMF
                     TK"
BASE COUNT          426 a          285 c          227 g          470 t
ORIGIN      
        1 cgatgagcca tcgcaattgt cgtatgataa tgccacctat tatcaaccaa atatgtacag
       61 gtattccgac cacgacccta ttgtagttgg gttgaagcta acaccacaag ctccatttga
      121 acccttggat aaaattcaat tatttcacga tttgacatcc aaacatatca ttgtaacacg
      181 tgcaaaaaat caccaagtgt ctattattgg aattgatggg cgttttgctc acttccactc
      241 aattgaatcg gatacagaaa caataaacac caacatgttg ccagctggtg tttatgttgt
      301 aaaaatcaca ggaacgaaag aagaaacgtt taagtttatt caattataga atactaccct
      361 attttacggc aatcgtttca atttctacca ttacccccaa tggtagttct tttacagcta
      421 cagtagaacg agcaggaaat ttttcagtat aatatttttt gtacacttcg tttacaaccg
      481 aaaaattctt catgtctgtt ataaaaatag tggattttac tacatctgaa tagccgtagc
      541 cagctgcttg caaaatggct ccaatatttt gaagtacttg ctctgcttgt tgctctattg
      601 taagagcatc aactttacca ttttctgggt ttacaggtat ttgaccagaa atatacaagg
      661 tgtcatttac ttcaatagct tgactgtaag gacctattgc tgctggagct tttgttgttg
      721 aaattattcg tttcatattt tttattattt agtaaacatt tcgttaatcg ctgtttctat
      781 ctgctgaaga gcttgttgta atacactacg tggacatgct acattcagtc gctgaaagcc
      841 ctctcctcct tgaccaaaag tagccccatc attcattatt acctttgctt tatgaaccat
      901 aaattctttt aattctttgg aagttaaacc catttttctg caatcaagcc atactaaaaa
      961 ggaagcatcg ggcacaacta cttgtatgat aggaatatgc tgttcaatat aattgcgtac
     1021 aaatagtatg ttttgttcaa tataatgtat agcttggttt aaccattctt tcccttcatt
     1081 gtaagccact tctgttgcca tacagccaaa tacatttccc atattgagat gaaaattagc
     1141 tgtttcttgc tgcattttgg caaaaagttg cggattctca gctacaaaaa aagcactagc
     1201 taaaccaggt atgttaaatg ttttactagg tgccatgaaa gtaatggtat gttgagcaat
     1261 ttcgggcgat aatttggcta aaggaatatg ttgtttaccc cacagcatca aatctgcatg
     1321 tatttcatcc gacaccatta acacattgta ctgaatacac aatttagcca tacgcatcaa
     1381 ctcgtcagca ttccacactc ttcctcct
//
LOCUS       DAOPUR010000392         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_107112_length_1399_cov_2.914435, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000392 DAOPUR010000000
VERSION     DAOPUR010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107112_length_1399_cov_2.914435"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKN90_06615"
     CDS             complement(<1..554)
                     /locus_tag="PKN90_06615"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNZ85983.1"
                     /translation="MKNLHIGGLVIPVPIIQGGMGVGISLSGLASAVANQGGVGVMSA
                     AGLGLLYRHLSSDYPEACILGLREEIKKAREKTKGIIGVNIMVAMTNFADMVKTSIAE
                     KVDIIFSGAGLPLDLPSFLQKDSTTKLVPIVSSARAAKLICDKWKAAYNYLPDAVVVE
                     GPKAGGHLGYKESQLSDDAYRLET"
     gene            complement(704..>1399)
                     /locus_tag="PKN90_06620"
     CDS             complement(704..>1399)
                     /locus_tag="PKN90_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNZ85984.1"
                     /translation="SIHEQLKQSIEHIQQDIEQVSSRFLSVFFSMFRNTPDYQDNAAL
                     QERIQSAASYFLVQLNTLVAPLIAAIKVDVDNKETEKQVNDLHAQLSETLQIHTACLS
                     SCKNNGFSVSAYQKAKLEAAIEHPSTKKRTAKASEKQKVSINSGETEHAQLYEHLRAW
                     RLQEASERKLPAYAILHQKALLGIVQHLPLSPTELLQIPHLGKRTVTKFGDALIEIVR
                     EFVQSKENDKTNS"
BASE COUNT          391 a          252 c          271 g          485 t
ORIGIN      
        1 atagtctcca atctgtaagc atcgtcagaa agttgagatt ctttatagcc taaatgtccc
       61 cctgcttttg gaccttctac caccacagca tctggcaaat agttgtatgc agctttccat
      121 ttgtcgcaaa ttaatttggc tgctctagct gacgaaacga tgggaactaa ttttgtggtg
      181 ctatcttttt gtaaaaatga aggtaaatcg aggggtaatc ctgcaccaga gaaaatgata
      241 tctacttttt cggctattga tgttttaacc atatcagcaa aattggtcat ggcgaccatg
      301 atatttacac caataattcc ttttgttttt tctcgtgctt tttttatctc ttcacgcaat
      361 cctaatatac atgcttcggg ataatctgat gataaatgtc ggtataacaa tcctagtcct
      421 gcagcagaca taacacctac tcctccttga ttagctactg ctgaggctaa gcctgataat
      481 gaaataccaa cgcccatacc tccttgtata attggaacgg gaataactaa gcctccaata
      541 tgtaaattct tcatatagta tatataaaat ggtgtgcaaa ggtagtgtta atttgcggta
      601 tatttcgcat gcggatttaa ttggcgtcta ttcggcagaa aaaagccaca aataattgca
      661 cagataattt ttgaacagaa aattaattta cgtatatgtt tggttatgag ttagttttgt
      721 cgttttcttt tgattgcaca aattcgcgta caatttcaat taacgcatcg ccaaattttg
      781 taacagtacg tttgccaagg tgaggaattt gaagtaattc tgttggcgac aagggtaaat
      841 gttgcacaat acccaacaga gctttttggt gtaaaatggc ataggcaggt agctttcttt
      901 cagaagcttc ttgtagtcgc caagctcgta agtgctcgta tagttgtgca tgttcagttt
      961 ctccactatt gatggatact ttctgtttct cagatgcttt ggcagtacgt ttttttgtag
     1021 atggatgttc aatggcagct tctaatttgg ctttttgata agcactaacg ctaaacccat
     1081 tgtttttaca actgcttaaa caagcggtat gaatttgtag agtttcacta agttgagcat
     1141 gtaaatcgtt tacttgtttt tctgtctctt tgttatctac atctactttt atcgcagcaa
     1201 taagtggtgc taccaatgtg ttgagttgaa ccaaaaaata acttgctgcc gactgtatgc
     1261 gttcttgtaa tgctgcattg tcttgatagt cgggtgtgtt gcgaaacatc gaaaagaaaa
     1321 cagaaagaaa acgactactt acttgttcaa tatcttgttg tatgtgctca atggattgtt
     1381 ttagttgttc atgaatcga
//
LOCUS       DAOPUR010000393         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_107232_length_1398_cov_3.087863, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000393 DAOPUR010000000
VERSION     DAOPUR010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107232_length_1398_cov_3.087863"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1398
                     /gene="scpA"
                     /locus_tag="PKN90_06625"
     CDS             <1..>1398
                     /gene="scpA"
                     /locus_tag="PKN90_06625"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNZ85985.1"
                     /translation="LNKMSVSMTMNGAVLPVLAFYINAGLEQGAKLEELSGTIQNDIL
                     KEFMVRNTYIYPPKFSMKIIADIFEYTSKNMPKFNSISISGYHMQEAGATADIELAYT
                     LADGLEYLRAGVNAGMDIDSFAPRLSFFWAIGMNHFMEIAKMRAARLLWAKIVKQFNP
                     KNPKSLALRTHSQTSGWSLTEQDPFNNVGRTCIEAMAAALGHTQSLHTNALDEAIALP
                     TDFSARIARNTQIYIQQETDICREVDPWAGSYYVESLTNELAEKAWALIEEVEKLGGM
                     AAAIETGIPKMRIEEAAARTQARIDSGQQTIVGTNKYRLEKEDPLDILEVDNTAVRIQ
                     QIERLTQLKQARDNAAVKNALAAITKCVETGEGNLLELAVEAARVRATLGEISDACEV
                     IVGRYKATIRTITGVYSAGTKNDADFIKACELTAKFAEKEGRQPRIMIAKMGQDGHDR
                     GAKVVATGYADCGFDV"
BASE COUNT          446 a          247 c          315 g          390 t
ORIGIN      
        1 ttgaataaaa tgtctgtatc aatgacgatg aacggtgctg tattgcctgt gttagctttt
       61 tatatcaatg caggattaga gcaaggtgct aaacttgaag aattgtcagg tactatccaa
      121 aatgatattt tgaaagaatt tatggtgcgt aatacttata tttatccacc taaattttcg
      181 atgaaaatta ttgctgatat ttttgaatat acgtcaaaaa atatgcctaa attcaactca
      241 atttctattt cgggatacca catgcaagaa gctggtgcca ctgctgatat tgagttagca
      301 tataccttgg cagatggatt ggagtactta cgtgcgggtg taaatgcagg tatggatatc
      361 gattcgtttg ctcctcgttt atcatttttc tgggcaatag gcatgaatca ctttatggaa
      421 attgctaaaa tgcgtgcagc tcgtttgcta tgggcaaaaa ttgtaaaaca atttaatcct
      481 aaaaacccaa aatctttggc tttgcgtacc cactcgcaaa cttctggatg gtcgctaaca
      541 gagcaagatc catttaataa cgttggtcgt acgtgtattg aggcaatggc ggctgccttg
      601 ggacatacac aatcattgca caccaatgca cttgacgagg ctattgcttt accaacagat
      661 ttttcggctc gtattgctcg taacacacaa atttatattc aacaagaaac ggatatttgt
      721 cgcgaagttg acccttgggc aggttcgtat tacgtagagt ctttaacgaa tgaattggct
      781 gaaaaagcgt gggctttaat tgaagaagtt gagaaattgg gtggtatggc tgctgctatc
      841 gaaacaggta ttccaaaaat gcgtatcgaa gaggctgctg ctcgtaccca agctcgtatc
      901 gactcaggtc agcaaaccat tgttggaacc aacaaatacc gtttagaaaa agaagatcct
      961 ttggatattt tagaagttga taatacagcc gtacgcatac aacaaattga gcgtttaact
     1021 caattgaaac aagccagaga taatgccgct gtaaaaaatg ccttagctgc cattaccaaa
     1081 tgtgtagaaa caggcgaagg aaacttgttg gagttagctg tagaagcagc tagagtgcgt
     1141 gcaacattgg gcgaaatctc agatgcatgc gaagtgatag taggaagata taaagcaacc
     1201 attagaacca taacaggagt gtatagtgca ggaacaaaaa atgacgcaga ctttattaaa
     1261 gcgtgcgaat taacagcaaa atttgctgaa aaagaaggtc gtcagccacg tattatgatt
     1321 gccaaaatgg gtcaagacgg acacgaccgc ggtgcaaaag ttgtagcaac aggttatgcc
     1381 gattgcggat ttgacgtg
//
LOCUS       DAOPUR010000394         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_107572_length_1395_cov_2.967164, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000394 DAOPUR010000000
VERSION     DAOPUR010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107572_length_1395_cov_2.967164"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PKN90_06630"
     CDS             <1..984
                     /locus_tag="PKN90_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNZ85986.1"
                     /translation="MASDFEMDFSDVKGQETVKRAMEVAAAGGHNIIMIGPPGAGKSM
                     LAKRIPSILPPLTLHEALETTKIHSVAGKIEKGISLMYKRPFRSPHHTISDVALVGGG
                     SFPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRKICISRSKFSIEYPASFML
                     VSSMNPCPCGYYNHPEKDCVCGPGIVQKYLNRISGPLLDRIDIHIEIVPVPFEKLSCA
                     KPTEHSEVIRERVIKARKIQEERFKENDGVHCNAQMSSKLLREHTKLSDTSQQLLKVA
                     MQRLNLSARAYDRILKVARTIADLDDAKDIQVEHISEAINYRNLDREGWAG"
     gene            1012..>1395
                     /locus_tag="PKN90_06635"
     CDS             1012..>1395
                     /locus_tag="PKN90_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNZ85987.1"
                     /translation="METVLSGIRSTGNLHLGNYYGALRNFIKMQEEYQCFFFIADYHS
                     LTTHPNPHEMHGNVRNVLTEYLAAGIDPDKATIYVQSDVPEIPELYLLLNMHAYLGEL
                     SKTASFKEKARKQPENVNAGLLTYPT"
BASE COUNT          477 a          284 c          281 g          353 t
ORIGIN      
        1 atggctagtg attttgaaat ggatttttcg gatgtaaaag gacaagaaac cgtaaaacgg
       61 gctatggaag tggcagctgc aggtggacac aatattatta tgattggacc tccaggtgct
      121 ggaaaatcga tgttggcaaa acgcattcca tctatcttgc caccacttac cttacacgaa
      181 gcattagaaa caacaaaaat acattcggta gctggaaaaa ttgaaaaagg tatatcgttg
      241 atgtacaaac gtccgtttcg cagtccgcac catactattt ctgatgtagc actagttggc
      301 ggagggagtt ttccacaacc aggcgaaatt tcgttggctc ataatggggt attgttttta
      361 gacgagctac ccgaatttaa acgaaccgta ttagaggtaa tgcgtcaacc actcgaagat
      421 agaaaaattt gcatctcacg ctcaaaattt agcattgagt atccagctag ttttatgttg
      481 gtatcatcaa tgaatccatg cccatgtggg tactataatc acccggagaa agattgtgta
      541 tgcggacctg gtattgtgca aaaatacctc aaccgcattt caggaccact gctcgaccgc
      601 attgacatac acatcgaaat tgtgcctgtt cctttcgaaa agttgtcatg tgccaaacct
      661 acagaacaca gcgaagtaat acgcgaacgt gtaataaagg cacgaaaaat acaagaggaa
      721 cgttttaaag agaatgatgg cgtacactgc aatgcacaaa tgtcatcgaa attgctcaga
      781 gagcacacca aactcagcga tactagtcaa caattattaa aagttgccat gcaacggctc
      841 aatctttcag caagagcata cgaccgaata ttaaaagtag cccgaacaat agccgattta
      901 gatgatgcaa aggatattca agtagaacat atatccgaag ccatcaatta tcgcaatctc
      961 gacagagaag gttgggcagg ttaaataaaa aacttaaaaa aaatagatac tatggaaaca
     1021 gtacttagtg gcattaggtc aactggaaat ttacatttag gaaattatta tggtgcttta
     1081 cgcaatttta taaaaatgca agaagaatac cagtgtttct tttttatagc cgattaccac
     1141 tcgcttacta cgcatccaaa cccacacgaa atgcatggca acgtacgcaa tgtgttgact
     1201 gaatacctag cagcaggcat tgaccccgac aaggcgacta tttacgtgca aagcgatgta
     1261 cctgaaattc ctgagttata ccttttactc aacatgcatg cctatttggg agaactctca
     1321 aaaacagcct catttaagga aaaagcccgc aaacaaccag aaaatgtgaa tgctggatta
     1381 ttaacgtatc caacc
//
LOCUS       DAOPUR010000395         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_108062_length_1391_cov_2.976796, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000395 DAOPUR010000000
VERSION     DAOPUR010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108062_length_1391_cov_2.976796"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="PKN90_06640"
     CDS             <1..1390
                     /locus_tag="PKN90_06640"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNZ85988.1"
                     /translation="LNNEQISDYIHYYEKQLATIKALKFVPASGAASRMFKRLSEFLE
                     AHVHNAHYLRTYYPDVAAFMHHLSKFAFYEELKTCIEKDGETIEDLLQKEQYNKIILY
                     LLTPLGLNYGNLPKALLSFHRYAENTRTAFEEHLVEGVAYAKNVQNEVAVHFTISPEH
                     KSAFIEKMNHVLPIYEDAFSVTFKLSFSEQKPSTDTIAVNENNEIIRNEDGSMLFRPG
                     GHGALIENLNDCDADIIFIKNIDNVVVDTLKQSTYTYKKALAGLLLSIQAATFDLLKK
                     LDGNVDDATLKIIEDFAKNTLYIHVPSQYAVAPKEEKIAFWKKSLNRPIRVCGMVKNE
                     GEPGGGPFWVLNEQNEESLQVVESSQIDYKNKLQERIAVKASHFNPVDIVCATKNMHG
                     EKFYLPDFVDPKTGFISSKSKDGKTLKAQELPGLWNGAMANWISIFVQVPISTFNPVK
                     TVNDLLRKEHQA"
BASE COUNT          510 a          268 c          248 g          365 t
ORIGIN      
        1 gttaaacaac gaacaaattt cagactatat tcattattac gaaaaacaac tagctacaat
       61 caaagctctt aaatttgtac ccgcttctgg tgctgcgtct cgtatgttca aaagattatc
      121 tgaattttta gaggcacatg tgcacaatgc ccattattta cgtacgtatt accctgatgt
      181 agccgccttt atgcatcatt tatctaaatt tgccttttac gaagagctaa aaacctgtat
      241 tgaaaaagat ggagaaacca tcgaagatct cttacaaaaa gaacaataca acaaaattat
      301 tttgtatttg cttacccctc tcggacttaa ctatggcaat ttgcccaaag ccctactatc
      361 gtttcatcgg tatgccgaaa acacccgcac agcattcgaa gaacatttgg tagaaggagt
      421 ggcgtatgca aaaaatgtac agaatgaagt tgctgtgcat tttaccattt cgcccgaaca
      481 caaatcagct tttatcgaaa aaatgaacca cgtattaccc atatacgagg atgcatttag
      541 tgtaacattc aaactatctt tttctgagca aaaaccatca acagatacca tagcagtaaa
      601 cgagaacaac gaaattattc ggaatgaaga tggtagtatg ctttttagac caggaggaca
      661 cggagcgttg attgaaaact taaacgattg tgatgcagat attattttca taaaaaatat
      721 tgacaatgta gtagttgaca ccttaaaaca atcgacctac acctataaaa aagccttagc
      781 aggcttactt ctgagcatac aagcagcaac tttcgatcta ttaaaaaaac tcgatggcaa
      841 cgtagatgat gccacactta agataataga agattttgcc aaaaacaccc tatatattca
      901 cgtacctagt caatatgcag tagcaccgaa agaggagaaa attgcttttt ggaaaaaatc
      961 cctaaacaga ccgatacgtg tgtgtggtat ggtaaaaaat gagggagaac ctggtggagg
     1021 tccattttgg gtgctaaacg aacagaatga agaaagttta caggtagtag aatcatcgca
     1081 aattgactac aaaaacaagc tacaagaacg cattgcagta aaagcaagcc attttaaccc
     1141 tgtggatatt gtctgtgcca caaaaaatat gcatggagaa aaattttatt tacccgattt
     1201 tgttgacccc aaaactggat ttatttcctc caaatcgaaa gacggaaaaa ccttaaaagc
     1261 acaagaattg ccaggattat ggaacggagc aatggcaaac tggataagca tatttgtaca
     1321 agtacctatc agcacattta atccagtaaa aacggtaaat gacctgctta gaaaagaaca
     1381 ccaagcctaa a
//
LOCUS       DAOPUR010000396         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_108449_length_1388_cov_2.759190, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000396 DAOPUR010000000
VERSION     DAOPUR010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108449_length_1388_cov_2.759190"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKN90_06645"
     CDS             complement(<1..568)
                     /locus_tag="PKN90_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ85989.1"
                     /translation="MGNNLLKGKRGIIFGALNDMSIAWKVAERVVEEGATITLTNTPL
                     AVRMGTTNELGKKLNAQIIPADATNVADLENVFAESIKILGGKIDFVLHSIGMSPNVR
                     KGRAYDDLDYGMLSTTLDISAISFHKVLQVAKKMDAINEYGSVIALSYMAAQRTVCGY
                     NDMADAKALLESIARSFGYIYGRDKKVRI"
     gene            complement(659..>1388)
                     /locus_tag="PKN90_06650"
     CDS             complement(659..>1388)
                     /locus_tag="PKN90_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNZ85990.1"
                     /translation="KAYFNEKFQEQAIDARYDNYLLPTIKDVMTILNDTPNLCGLNVT
                     IPYKEQVMEYLDEIDDEAKEIGAVNVIKITEKNGKRFLKGYNSDWFGFTEAIKPFLKP
                     HHTHALILGTGGASKGILYALKKLKINTQFVSRDPSKGLTYKQLTKEILQQYTIIVNT
                     TPVGTFPEVKHCPPIPYHFLTDKHLVYDLIYNPIRTLFLHQAETRGATTCNGWQMFLN
                     QAHKAYVIWEGKDLITNKKHVEKI"
BASE COUNT          390 a          249 c          230 g          519 t
ORIGIN      
        1 tgatacgtac ttttttatca cgaccatata tataaccaaa acttcgtgca atggattcga
       61 gcaatgcttt tgcatcagcc atgtcgttgt atccacatac agtgcgttga gcagccatat
      121 acgataaagc tattacagaa ccatattcat tgatagcatc catttttttt gctacttgca
      181 atactttgtg aaaagaaata gcagatatat caagggtagt actaagcatt ccatagtcta
      241 aatcatcgta agcacgacct ttgcgaacat ttggtgacat accaattgaa tgcaagacaa
      301 aatcaatttt accacctaag atttttattg attcagcaaa tacattttcc aaatcggcta
      361 cgtttgtagc atcggctggg ataatttgag catttaattt cttgcctaat tcgttagttg
      421 tacccatacg tacagctaaa ggtgtgttgg taagagtaat ggtagcacct tcttctacta
      481 ctctttcagc tactttccat gcaatagaca tatcgtttaa tgcaccgaag ataattcctc
      541 tttttccttt tagtaagtta tttcccatgt tttctgttta ttaaaattgc acaaaattac
      601 tccttttgtt caataagttc caaactaaat ccctcttttt ttattttttc tatcaatctc
      661 atattttttc aacatgcttt ttgtttgtaa taaggtcttt gccctcccaa attacataag
      721 ccttatgtgc ttgatttaaa aacatttgcc atccgttaca ggtcgttgct cctcgtgttt
      781 cggcttggtg tagaaaaagc gtacgtattg ggttgtagat taaatcatat actaagtgtt
      841 tatctgtaag aaaatgatag ggtataggag gacaatgttt tacttcgggg aatgttccta
      901 cgggggtggt gttcacaata atagtgtatt gttgcaaaat ttcttttgtt agttgcttgt
      961 atgtaagtcc tttagatggg tctcgtgata caaattgtgt attaatcttt aattttttta
     1021 gagcatatag aatccctttt gaggctcctc ctgtacctaa tattagggcg tgtgtatggt
     1081 ggggtttcaa aaaaggtttg atagcttctg taaagccaaa ccaatcagaa ttgtatcctt
     1141 tcaaaaaacg ttttccgttt ttttctgtaa tttttattac attcacagct ccaatctctt
     1201 ttgcttcgtc atcaatttcg tccaaatatt ccattacttg ctctttgtat ggtatggtta
     1261 catttaaacc acacaaatta ggagtatcat tcaatattgt cattacatct ttaatggtgg
     1321 gtagtaaata attgtcatac ctggcatcaa tagcttgttc ctgaaatttc tcattaaagt
     1381 aagctttt
//
LOCUS       DAOPUR010000397         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_110869_length_1368_cov_3.327494, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000397 DAOPUR010000000
VERSION     DAOPUR010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110869_length_1368_cov_3.327494"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..>1368)
                     /locus_tag="PKN90_06655"
     CDS             complement(159..>1368)
                     /locus_tag="PKN90_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNZ85991.1"
                     /translation="QHLVGEGAILRKMIESGSITSFILWGPPGVGKTTLARIIASKMN
                     RGFYALSAVNSSVKDIREIIEKAKKTQFFDTPSPILFIDEIHRFSKSQQDSLLAAVEN
                     GTVMLIGATTENPSFEVITPLLSRCQVYVLKHLEEQDLRSLLNRVLEKDAVCKSKKIV
                     VQETDALFRFAGGDARKLLNIIELLIGTENQTEIVLTNKLVTEKLQENLAIYDKGGEM
                     HYDIISAFIKSIRGSDPDAAIYWLARMIDGGEDPKFIARRLLISATEDIGLANPNALL
                     LANTCFDAVHKIGFPESRIILAETTIYLASSKKSNSAYLAIDKALDIVRKTGNLSVPL
                     HLRNAPTSLMKNLEYGKNYVYPHDYPQNFTPQTYLPQELEKQSIWESQSNSTEKNLSE
                     WLRSLWKNRF"
BASE COUNT          419 a          279 c          234 g          436 t
ORIGIN      
        1 attgcaacag catggatact ccgacaccca gctaaaatgc aagttgtggc tggcaccacc
       61 aaaccaaata ggttacaaga aatttgtgaa ggtaccacgg taacacttac gcgtgaggaa
      121 tggtatcaaa tctatctagc tgctggaaat acattgcctt aaaaacgatt tttccacaac
      181 gaacgcaacc actccgacag gtttttttcc gtactattcg attgactttc ccatattgat
      241 tgcttttcaa gttcttgtgg caagtacgtt tgtggggtga aattctgtgg ataatcgtgc
      301 ggatacacgt aatttttgcc atactctaag tttttcatca aggatgtagg agcattacgc
      361 aaatgcaatg gaacggaaag atttcctgtt ttacgaacaa tatcaagggc tttatcaatt
      421 gccaaatagg ctgaattgct ttttttacta ctcgccaaat atatcgttgt ttcagctaat
      481 ataatgcgtg attcgggaaa accaatttta tgtacagcat caaaacaagt atttgctagt
      541 aacaatgcgt tcggattagc taatccaata tcttctgtag ctgaaataag caacctgcga
      601 gctataaact tggggtcttc tcccccatct atcatacgag ctagccaata aattgctgca
      661 tcaggatcgc ttcctctaat cgatttaata aacgcagaga taatatcgta atgcatttcg
      721 ccgcctttgt cgtaaatagc caagttttct tgtaattttt cggttacgag tttattcgta
      781 agaactatct ctgtttggtt ttctgtacca attaggagtt caataatatt gagcaactta
      841 cgtgcatctc cacccgcaaa acggaacaaa gcatcggttt cttgaacaac aattttcttg
      901 cttttacaca ctgcatcttt ttctaatact cgattgagca aagagcgtaa atcttgctct
      961 tctaagtgtt ttaacacata tacctgacac ctagataata aaggagtaat tacttcaaat
     1021 gatggatttt ctgttgtggc tccgataagc ataactgtac cattttctac ggcagccaat
     1081 aacgaatcct gttgtgattt actaaaacga tgaatctcat caataaaaag aataggacta
     1141 ggtgtatcaa aaaattgcgt tttttttgct ttttcaatta tttctcgtat atcttttaca
     1201 ctcgaattta cagcactcaa ggcataaaaa cctctattca tttttgaagc aatgatacgt
     1261 gccaaggttg ttttaccaac acctggagga ccccataaaa taaaagacgt tatactaccc
     1321 gattctatca ttttacgcaa aatagcacct tcaccaacta aatgttgt
//
LOCUS       DAOPUR010000398         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_111150_length_1366_cov_2.505721, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000398 DAOPUR010000000
VERSION     DAOPUR010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111150_length_1366_cov_2.505721"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1366
                     /gene="pulA"
                     /locus_tag="PKN90_06660"
     CDS             107..>1366
                     /gene="pulA"
                     /locus_tag="PKN90_06660"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HNZ85992.1"
                     /translation="MRKINLFLVSVIALLLMSCVNLPKEYASYDDYPVYEGNDLEVVY
                     SPLHTKFRLWSPAAKEAKLNLYHEGLGGEPFDVVKLKYDIDGTWVATVDGDLKGKFYT
                     FQIFQQDRWWDETPGIWAKAVGVNGNRGAIIDMSETNPSGWEDVKRPAMKNFTDAVLY
                     ELHVRDFSTSEQSGMQYKGKFLAFTEMGTVNAEGKKTGVEHLKELGITHVQLLPIYDY
                     ASVDETKLDENQYNWGYDPKNYNVPEGAYSTDPYNPTARIIELKKAIQSLKNNGIRVI
                     MDVVYNHTFWGVQSHLNLTAPGYFYRFKEDGSWSNASGCGNETASERAMVRKYIIESC
                     KYWVNEYKVDGFRFDLMGIHDIETLNLLRAELDKIDPSITIHGEGWTAEWDFPLPFEK
                     RAIKQHAKQLNNIAVFSDDIRDGIRGTW"
BASE COUNT          456 a          195 c          289 g          426 t
ORIGIN      
        1 tgcgaattac gtttgatttt tttgaatacc taaaacccta gcaaagtgaa agcaattcta
       61 ctagtattag taacaacatt tttatatcag aataagaaaa aagagcatga gaaaaataaa
      121 tctatttttg gtttccgtaa ttgcgttatt gcttatgtcg tgtgttaatt tgcctaagga
      181 gtacgcttcg tatgatgact atccagtata cgaaggcaat gatttagagg tcgtatactc
      241 ccctttacat actaaattta ggctatggtc acctgctgca aaagaagcca aattaaatct
      301 atatcatgaa ggtctgggag gagagccttt tgatgttgta aaattaaaat atgatataga
      361 tggtacttgg gtagctactg ttgatggcga cttaaagggt aagttttata cttttcaaat
      421 atttcagcaa gatagatggt gggacgaaac tcctggaatt tgggcaaaag ctgttggtgt
      481 aaatggaaat aggggagcta tcattgatat gtcggaaacc aatccctctg gttgggaaga
      541 tgtgaaaaga ccagcaatga aaaattttac tgatgctgtg ctttacgagt tgcatgtgcg
      601 tgatttttct acatctgaac aatcgggtat gcaatataaa ggaaagtttt tagcttttac
      661 cgagatgggg actgtaaatg ctgaaggtaa aaaaacgggc gttgaacatc taaaggaatt
      721 aggtataaca catgtacaac ttttaccaat atatgattat gcctctgtgg atgaaacaaa
      781 attagatgaa aatcagtaca actgggggta cgatcctaaa aattataatg ttcctgaagg
      841 agcttattct accgacccat ataatccaac ggctcgaatt attgaattaa agaaagccat
      901 tcaatcattg aaaaataatg gtatccgtgt gattatggat gttgtttata atcatacctt
      961 ttggggagtt caatcgcatt taaatttaac tgctcctggg tatttctatc gttttaaaga
     1021 agatggaagt tggtctaatg catcaggatg cggtaacgaa acagcctcag aaagagcaat
     1081 ggttcgtaaa tatattattg aatcgtgtaa atattgggta aatgaataca aagtagatgg
     1141 ttttcgattt gatttgatgg gtatccatga tattgaaaca ttaaatttac tacgtgcaga
     1201 attagacaaa attgacccct ctattactat acatggtgaa ggatggacag cagagtggga
     1261 tttcccctta ccttttgaga aacgtgctat taaacaacat gccaaacaat taaataatat
     1321 tgctgtattt agtgatgata ttcgtgatgg cataagagga acttgg
//
LOCUS       DAOPUR010000399         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_113059_length_1352_cov_2.473400, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000399 DAOPUR010000000
VERSION     DAOPUR010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113059_length_1352_cov_2.473400"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKN90_06665"
     CDS             complement(<1..477)
                     /locus_tag="PKN90_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ85993.1"
                     /translation="MNKPIISIISSIAGICTLLFIVLHLAVNLLLVLDAIANTQGYYY
                     NSMHYFMQHNSVAYTLEKTFALGFVLHILLANILAFNKIRNKQGSKDMIIMGFLLLIF
                     TVLHVLQFKVKRTIEYVELDGVLKPDIYQFISHFFSSNWQYSLLYMFSGILLGVHLQ"
     gene            complement(544..>1352)
                     /locus_tag="PKN90_06670"
     CDS             complement(544..>1352)
                     /locus_tag="PKN90_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ85994.1"
                     /translation="DFIEVLPKVALHYKIDNQHRVYTSLTKGYKAGGYNYSMFSDILQ
                     AQMQGNKDVTIDDKIYYKPEESWNIEVGSNGTFGNNRFNYQTAIFYIHHYNQQLSTTT
                     SNGSRMISNAEVSQSIGGELSVKAYLSKQIAGYIHYGHTNASFIKYNANNINYNNKSI
                     PFAPKNTLSLGIETKVPITEEWLDEIVLSAHYTGIGPLYWNAQNTLSQEYYGIANGDI
                     TFSKNNTHLKFWVKNALNQSYNVFYFESLGNSFVQKGIPISFGIHVKHIL"
BASE COUNT          445 a          213 c          206 g          488 t
ORIGIN      
        1 ttgtaaatgc acaccaagta atatcccaga aaacatatat agcaaggagt attgccaatt
       61 actcgaaaag aaatgactga taaactggta gatatcaggc tttaataccc catctaactc
      121 tacgtattct attgtccgtt ttactttaaa ttgcaataca tgcaataccg taaatatcaa
      181 taaaagaaat cccataatta tcatgtcttt agaaccttgc ttgtttcgta ttttgttaaa
      241 ggctagtata tttgccaata aaatatgtaa cacgaaaccc aatgcaaaag tcttttctag
      301 ggtatatgct acactattat gttgcataaa atagtgcatt gaattgtaat aatatccctg
      361 tgtgttagca atagcatcta gtacaagtaa taagttaaca gctaaatgta atacaataaa
      421 taagagcgtg caaatacctg caatactaga tatgattgaa ataattggtt tattcatttt
      481 tttaatctaa aaaatacaaa gatgcaaatt taatcaaaaa gatgcatctt tgtataaatg
      541 gggttacaaa atgtgtttca catgaatgcc aaaagatatc ggaatacctt tttgtacaaa
      601 actatttcca agtgattcaa aataaaacac attgtacgat tgattgagtg catttttgac
      661 ccaaaatttc agatgtgtat tattttttga gaatgttata tcgccatttg caataccata
      721 atattcttga gaaagcgtat tttgtgcatt ccagtatagt ggaccaatgc ctgtgtaatg
      781 tgcagaaaga acgatttcat cgagccattc ttcggttatt ggtaccttag tttcaatacc
      841 taaagagagt gtatttttag gtgcgaaagg tattgattta ttgttgtaat taatgttatt
      901 cgcattgtat ttaataaagc ttgcatttgt atgtccgtaa tggatatacc ccgctatttg
      961 tttacttaaa tatgccttta cacttagttc tccacctata ctttggctta cttctgcatt
     1021 gctaatcata cgacttccat tgctagtggt tgtactcaat tgttggttat agtgatgaat
     1081 ataaaaaatt gcagtttggt aattaaaacg attgttaccg aatgtaccat tactaccaac
     1141 ctcaatattc catgattctt cgggcttata atatattttg tcatcaatcg ttacatcttt
     1201 gtttccttgc atttgtgctt gtaaaatatc agaaaacatc gaataattat atcctccagc
     1261 tttatatcct ttggttaatg aagtatatac tctgtgttga ttatctattt tatagtgtaa
     1321 tgcaactttc ggtaatactt ctataaagtc tt
//
LOCUS       DAOPUR010000400         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_114507_length_1341_cov_3.237170, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000400 DAOPUR010000000
VERSION     DAOPUR010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114507_length_1341_cov_3.237170"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1341)
                     /gene="infB"
                     /locus_tag="PKN90_06675"
     CDS             complement(<1..>1341)
                     /gene="infB"
                     /locus_tag="PKN90_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNZ85995.1"
                     /translation="PKEQISDEDIQKQIKETLARLTNKGKSKGAKYRKDKREAVSQRL
                     QEEADLERLESRVIKITEFVTANELATMMDVPVTQVIGTCMSIGIMVSINQRLDAETI
                     NIVAEEFGFKTEYVSAEVAQAIHVEEDAEEDLLPRAPIVTVMGHVDHGKTSLLDYVRR
                     ANVIAGEAGGITQHIGAYNVTLDSGQKITFLDTPGHEAFTAMRARGAKVTDIAIIIVA
                     ADDDVMPQTIEAINHASAANVPMVFAINKVDKPNANPEKIKEALAGMNYLVEEWGGKY
                     QSHDISAKQGMGVKELLEKVLLEAELLDLKANPNKKAIGSIVESSLDKGRGYVSTVLV
                     QTGTLKMGDVIIAGTHFGKIKAMFNERNQRITEAKPSEPVLILGLNGAPQAGDEFHVL
                     ETEQEARDIANKREQLQREQSIRTKKHLTLDELGRRIALGNFQELNVIVKGDVD"
BASE COUNT          353 a          299 c          207 g          482 t
ORIGIN      
        1 catccacatc tccttttaca ataacattta attcttggaa attgcctaat gcaatacgtc
       61 ttccaagttc atcaagcgta agatgctttt tagtacgtat actttgctcg cgttgcaatt
      121 gctctctttt gttggcaata tcacgagcct cctgttctgt ttctaataca tgaaattcat
      181 cccctgcttg cggtgcccca tttaatccaa gtattaaaac tggttcagaa ggtttagctt
      241 cagttattcg ttggttcctc tcgttaaaca ttgccttaat tttaccaaaa tgagtaccag
      301 caattatcac atcacccatt ttgagtgtgc ctgtttgtac gagtactgta gagacatacc
      361 ctctaccttt atccaaagat gattcgacaa ttgaacctat tgctttttta tttgggttcg
      421 cttttaagtc taataattct gcttcgagca atactttttc gagcaattct tttaccccca
      481 tcccttgttt ggcagatata tcatgcgatt gatatttacc tccccattcc tctacaaggt
      541 aattcatacc agctagggct tctttaattt tttcagggtt agcatttggt ttatctactt
      601 tatttatagc aaaaaccata ggtacattgg cggcagaagc atgattaatt gcttcaatag
      661 tttgaggcat aacatcatca tcagcagcta caataataat agcaatatct gttactttag
      721 cacctctagc acgcatagct gtaaaagctt cgtgaccagg agtgtctaag aatgtaattt
      781 tttgtccact atcaagagtt acattatatg ccccaatatg ttgggtaata cctccagcct
      841 ctcctgcaat cacattagct cgacgcacat agtctaacaa agatgtttta ccatggtcta
      901 cgtgacccat aacagttaca attggcgcac gtggtaataa atcctcttct gcatcctctt
      961 ctacatgaat tgcttgtgca acttcggcac tcacatattc tgttttaaat ccaaattctt
     1021 cagccactat attaatagtt tcagcatcaa gacgttgatt gattgaaacc ataattccaa
     1081 tactcataca agtaccaata acctgtgtta caggcacatc catcattgtc gccaactcat
     1141 tggctgtaac aaactctgtg atttttatta cgcgactttc taatctttct aaatcagctt
     1201 cttcttgtag acgttggctt actgcttcac gtttgtcttt acggtattta gctccttttg
     1261 attttccttt attagtcagt cgagcgagcg tttcttttat ttgtttttga atatcttcgt
     1321 cactaatttg ctctttagga a
//
LOCUS       DAOPUR010000401         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_114539_length_1341_cov_2.609642, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000401 DAOPUR010000000
VERSION     DAOPUR010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114539_length_1341_cov_2.609642"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /gene="rpoN"
                     /locus_tag="PKN90_06680"
     CDS             <1..1192
                     /gene="rpoN"
                     /locus_tag="PKN90_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNZ85996.1"
                     /translation="DYMPQDPNNMPASVYNIPYSEGESFHEYLLNQLHLLPLSKTQLL
                     LAEYIVGNIDDEGYLRREVVQIVTDISVLTGVHYTDSQLYEVLAIVQQLEPAGVGARS
                     LQESLLLQLQRKQLTPSIDLAMQILEDCFIEFSSKHYEKISAKLAIDNESLRDAIDEI
                     IHLNPKPGNGWEGILEKNKEEITPDFILEVQDNQLTLSLNNSTIPMLRVNQTYVNLFQ
                     DFVSNKANQTQSMKDAVLFAKQKLDAAKSFIDAVKQREETLLKVTSTIVNYQKKYFFE
                     GSDSFLRPMTMKDIAELSGYDISTISRVANSKYIQTNFGIFPLRHFFTEGMITQSGEE
                     VSTREIKNILSQTILSEDKSKPLSDDKLTEILQQKGYNIARRTVAKYREQLDIPVARL
                     RKEM"
BASE COUNT          476 a          198 c          237 g          430 t
ORIGIN      
        1 tgattacatg ccccaagacc ctaataatat gcctgctagt gtatataata ttccatattc
       61 agaaggagaa tcatttcatg aatatttatt aaaccaatta catttattac ccttatcaaa
      121 aactcaatta ttgttagccg aatatattgt tggtaatatt gatgatgaag gatatttacg
      181 tagagaagtt gtgcagatag ttacagatat aagtgtattg actggtgttc actatacaga
      241 ctctcagtta tacgaagtat tggctattgt gcagcaatta gaacctgctg gagtgggggc
      301 tcgttcgtta caagaatctt tgttattgca attgcaacga aagcagttga ccccatcaat
      361 tgatcttgct atgcaaattt tggaagattg ttttatagaa tttagtagta aacattatga
      421 aaagattagt gctaaattgg ctattgataa tgaatcctta cgggatgcta ttgatgaaat
      481 tatacatcta aatccaaagc caggaaatgg atgggaaggg atattagaaa aaaataagga
      541 ggaaattacg ccagatttta ttcttgaggt acaagataat caattaaccc tttcattgaa
      601 taatagtact attcctatgc tacgggtcaa tcaaacgtat gtaaatttat tccaagattt
      661 tgtatctaac aaggcaaatc aaacacaatc tatgaaagat gcagttttgt ttgcaaaaca
      721 aaaattagat gcagccaaat cctttataga tgcagtaaaa caaagagagg aaacattgct
      781 caaagtaact tcgacgattg taaattacca aaaaaagtat ttttttgaag gaagtgattc
      841 ttttcttcgt cctatgacga tgaaagatat tgctgagctc tctggttatg atatatctac
      901 catatcacgt gtagcaaata gtaaatacat tcaaacaaat tttggcattt ttccattacg
      961 ccattttttt actgagggga tgattactca atcaggagaa gaagtttcta caagagaaat
     1021 taagaatatt ttgagccaaa caattttgtc tgaagataaa tctaaaccct tgtctgatga
     1081 taaattaact gaaatattac aacaaaaagg atataatatt gctcgtagaa ctgtagcaaa
     1141 atatcgcgaa caattggata ttcctgtggc acgtttacgc aaagaaatgt gatagattat
     1201 cgtttaataa atttcacagg cgatattcct ttggcagttt caactaataa aatgtaccct
     1261 cctaaagaca actgacttat atctaaggtt atttcagtgc ttgtagatgt aattgtgtgt
     1321 gtaggaatag taatacccaa t
//
LOCUS       DAOPUR010000402         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_114889_length_1338_cov_3.855027, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000402 DAOPUR010000000
VERSION     DAOPUR010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114889_length_1338_cov_3.855027"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1338)
                     /locus_tag="PKN90_06685"
     CDS             complement(<1..>1338)
                     /locus_tag="PKN90_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNZ85997.1"
                     /translation="EPSQTITMQHAIDTPFWEPVIDDLQLVGNGAVVQQQSYWIIFVF
                     GFLGGLLALLTPCVWPMIPMTVSFFLKSSKNKNKAIKNALIYGFSIIIIYLGLGLLVT
                     GIFGASSLNSLATSAIFNILFFILLIVFALSFFGLFEITLPSSWVNAMDKKVDTTTEL
                     VSIFFMAFTLVLVSFSCTGPIIGTLLVEAASTGSVVGPAVGMFGFALALAIPFAFFSL
                     FPSYLQSMPRSGGWLNMVKVTLGFLELALALKFFSVADLAYGWGLLSRDLFIGIWIII
                     FIILGLYYLRVIRFPMDKDDKKITLPRIIFALVSFLFVVYLLPGMWGKQRASISAFTP
                     PMSTQIWNKNKDHVQAMFEDYDIAMKYAQTVDKPVLIDFSGYGCVNCREMEATVFEDD
                     AIKKMLKEDFIFVVLYVDDKKNLSEPMLVSENNATRTLRTQGDKWSYLQRLKFG"
BASE COUNT          505 a          262 c          190 g          381 t
ORIGIN      
        1 ccaaatttta aacgttgtaa atagctccat ttatctcctt gtgtacgcaa cgtacgcgta
       61 gcattatttt ctgacacaag cattggctcg ctaagatttt ttttatcatc aacatacaaa
      121 actacaaaaa taaaatcttc tttcagcatc tttttaattg catcatcttc aaatacagtt
      181 gcttccattt cgcgacaatt tacgcaacca taacctgaaa aatcaattaa aacaggttta
      241 tcaacggttt gagcatattt catagctata tcataatctt caaacatagc ttgcacatga
      301 tccttatttt tattccatat ttgtgttgac ataggaggtg taaatgcact tatagaggct
      361 cgttgttttc cccacatccc tggcagtaaa tagacaacaa ataagaaaga taccaaggca
      421 aagataatcc gtggaagagt tattttttta tcatctttat ccattggaaa acgtataaca
      481 cgcaaataat acaaacctaa aataataaaa ataataatcc aaatgcctat aaacaaatcc
      541 ctactaagca aaccccatcc gtaagccaaa tcggctactg aaaagaattt taatgccaat
      601 gctaattcta aaaaccctaa ggttaccttt accatgttta accatccccc agaacgtggc
      661 atagattgca gatacgatgg aaacaaggag aagaaagcaa aaggaattgc caacgccaaa
      721 gcaaaaccaa acatacctac tgcgggtcca acgaccgatc ccgtactagc cgcttctacc
      781 aaaagagtac caataatagg acctgtacaa gaaaaagaaa caagtactaa tgtaaatgcc
      841 ataaagaaaa tactaaccaa ttctgtagta gtatctactt ttttatccat tgcattgacc
      901 catgatgaag gtaatgttat ttcaaataag ccaaaaaatg acaatgcgaa tacaattaga
      961 agaataaaaa atagaatatt aaagatagca ctagtagcta aactattcag cgaacttgct
     1021 ccaaaaattc ctgttaccaa taaacctaaa cctaaataaa taatgataat agaaaaacca
     1081 tatatcaaag catttttaat tgctttgttt ttatttttag agctttttaa aaagaaacta
     1141 actgtcatag gtatcatggg ccatacacat ggggtaagta aggctaataa tccaccaaga
     1201 aatccaaaaa caaaaattat ccaatacgat tgttgttgta caacagctcc atttcctact
     1261 aattgtaagt catcaattac gggttcccaa aaaggagtgt ctattgcatg ttgcatggtt
     1321 atcgtttgtg atggttca
//
LOCUS       DAOPUR010000403         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_116960_length_1324_cov_3.066194, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000403 DAOPUR010000000
VERSION     DAOPUR010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116960_length_1324_cov_3.066194"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PKN90_06690"
     CDS             <1..646
                     /locus_tag="PKN90_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNZ85998.1"
                     /translation="REEQIEGDVGQVYRPDVIVLLPDNKHIIVDSKVSLLAYENFVNA
                     NDDEKDAYLKAHIVSLRNHIKGLGEKCYQNSPTLNSPDFVLMFLPIEGSFSVALQTDN
                     ELYSFAWDRKIVVVSPTTLLATLRTIASIWKQENQTKNAQEIARLGGALYDKLVGCVE
                     DLNKVKLNIDRASASHNDAMKKLQLGAGNVFVTAEKMKQLGAKTSKQLPEDLQE"
     gene            646..1080
                     /locus_tag="PKN90_06695"
     CDS             646..1080
                     /locus_tag="PKN90_06695"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNZ85999.1"
                     /translation="MLDDTYFMKQALAEAKKAFAKGEVPIGAVVVCNQQIIARAHNFT
                     ETLHDVTAHAEMQAITAAASYLGAKYLNDCILYVTVEPCVMCAGALYWSKVAKVVYGA
                     SEDKYGYTKKVPLALHPKTEVVAGVMADECAQIMKDFFITKR"
     gene            1085..>1324
                     /locus_tag="PKN90_06700"
     CDS             1085..>1324
                     /locus_tag="PKN90_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNZ86000.1"
                     /translation="MNFVDELEWRGMIHTVMPGTKEQLEKELTSAYVGIDPTADSLHI
                     GHLVGVMMLKHFQRCGHKPIALIGGATGMIGDPSGK"
BASE COUNT          433 a          219 c          296 g          376 t
ORIGIN      
        1 acgcgaagaa caaattgaag gggatgtagg tcaagtatat agacctgacg ttatcgtcct
       61 tttgcctgat aataagcata ttattgtgga ttcaaaggtg tcgttactag cgtatgaaaa
      121 ttttgtgaat gcaaacgacg acgagaagga tgcgtacctc aaggctcata tagtttctct
      181 tcgcaatcat ataaaaggac ttggcgaaaa gtgttatcaa aattctccaa cattaaattc
      241 tcctgatttt gtattaatgt ttttgcctat cgagggctct ttttcggtag cattacagac
      301 tgataatgag ctttatagct ttgcttggga tagaaagatt gtggtggtaa gtcctactac
      361 cttattggct actttgcgta ccatagcctc catctggaaa caagaaaatc aaaccaaaaa
      421 tgcccaagaa attgcacgtt tgggtggagc tttgtacgat aaattagtgg gttgtgtaga
      481 agatttaaac aaggtgaagt taaatattga tagagcctca gcttcgcata acgatgccat
      541 gaaaaaactg caattaggtg caggaaacgt atttgttact gccgaaaaaa tgaaacagct
      601 aggggcaaaa acaagtaaac aactacccga agatttgcaa gaataatgtt agacgatacg
      661 tattttatga aacaagccct ggcagaggct aaaaaagcgt ttgctaaagg agaagttcct
      721 attggtgcgg tggtggtatg taaccaacag attattgccc gtgctcataa ttttaccgaa
      781 acattgcatg atgttaccgc ccatgccgaa atgcaagcaa ttactgctgc tgcaagttat
      841 ttaggagcta aatacctgaa tgattgtatc ttatatgtaa cagtagaacc ctgtgtaatg
      901 tgtgctggtg cattgtattg gtcaaaagta gccaaagtag tatatggagc aagcgaagat
      961 aaatatggat ataccaaaaa agttccttta gccttgcatc ctaaaacgga agttgtggct
     1021 ggcgtgatgg cagatgaatg tgctcaaata atgaaagatt tttttataac taaacgataa
     1081 taacatgaat tttgttgatg aattagaatg gagaggtatg atacataccg taatgccagg
     1141 tacaaaagaa caattagaaa aagaattaac ctctgcttat gtaggtattg acccaacggc
     1201 agattccttg catataggtc atttggtggg tgtaatgatg ctcaagcatt ttcaacgttg
     1261 cggacacaaa cccattgcct taataggtgg tgcaaccggt atgattggcg atccatcggg
     1321 caaa
//
LOCUS       DAOPUR010000404         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_120178_length_1301_cov_2.334671, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000404 DAOPUR010000000
VERSION     DAOPUR010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120178_length_1301_cov_2.334671"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKN90_06705"
     CDS             <1..913
                     /locus_tag="PKN90_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ86001.1"
                     /translation="DEQHRFGVAQRAKLWNKNTPPPHVLVMTATPIPRTLAMTVYGDL
                     EVSVIDELPPGRKPIVTNHIFEDTKREALNRFLKQQLSLGRQVYIVYPLIEESEKMDL
                     KNLTDGYQYIQSIFPDLQVSMVHGKMKPAEKDAAMQEFVQHKSQIMVATTVIEVGVNV
                     PNASVMVIENAERFGLAQLHQLRGRVGRGADQSYCVLVTSRKISNETRKRLEIMTRTN
                     DGFEIAEADLQLRGPGDLEGTQQSGLPFQLHIANLAKDGHFVQLARECASEILENDPQ
                     LKSLANEIIVKELKTKKREQQNWSIIS"
     gene            998..>1301
                     /locus_tag="PKN90_06710"
     CDS             998..>1301
                     /locus_tag="PKN90_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNZ86002.1"
                     /translation="MEKTLVIIKPSAISRGLIGEVISRFEKKGLHLIGMKMIELSDEI
                     LAEHYAHLAQKPFFQRIKNAMQTTPVIVCCWSGLDAVQVVRNMAGVTNGRNAASGTI"
BASE COUNT          444 a          236 c          267 g          354 t
ORIGIN      
        1 tgatgaacaa catcgatttg gtgtggcaca acgagctaaa ttgtggaata aaaacacacc
       61 tcctcctcat gttttagtaa tgaccgctac tcccattcca cgtaccttgg ctatgactgt
      121 atatggcgat ttggaggttt ccgtaattga cgaattgcca cctggacgaa aaccaatagt
      181 aacaaaccat atttttgaag atacaaaacg agaggctctt aatcgttttt taaaacaaca
      241 actctcactt ggcaggcaag tatatattgt gtatccgttg atagaagaat ccgaaaagat
      301 ggatttaaaa aaccttacag atggctatca atacatacaa tcaatttttc cagatttgca
      361 agtaagcatg gtacatggca aaatgaaacc agctgaaaaa gatgctgcta tgcaagagtt
      421 tgtgcaacat aaatctcaaa ttatggtcgc tactactgtg attgaagtgg gggtgaatgt
      481 gccaaacgcc agtgtaatgg taatcgaaaa tgcagagcga tttggattgg cacagctcca
      541 tcagctcaga gggcgggttg gtagaggagc cgaccaatcg tattgtgtat tggtaacgtc
      601 gcgaaaaata tccaacgaaa cccgcaaacg cttagaaatc atgacccgta ccaacgacgg
      661 gtttgaaata gccgaggctg acttacagct tcgtggacca ggagatttgg aaggtaccca
      721 acagagtggc ttgccttttc aactacatat tgccaattta gctaaagatg gacattttgt
      781 tcaactagcc agagagtgtg cttcagaaat tctagaaaac gatccgcaac tgaagtccct
      841 agcaaacgaa attatagtaa aagaactcaa aacaaagaaa agagaacaac aaaattggag
      901 tataattagt tgatgtgtaa aaattctata atttttttgc aaaaaatttg gttaactaaa
      961 gaataaattg tttttttgta tgtcgtaaaa tcaaactatg gaaaaaacac tggttattat
     1021 taaaccttca gctatttcaa gaggtttaat tggagaagta atatctcgct tcgaaaagaa
     1081 aggtttacac cttattggga tgaaaatgat agaattatct gacgaaattc ttgccgagca
     1141 ttatgctcat ttagcccaaa aacccttttt ccaacgtatt aaaaatgcca tgcaaacaac
     1201 acccgttatc gtatgttgtt ggtcaggctt agatgctgta caggtagtaa gaaatatggc
     1261 gggagttacc aatggtcgta atgctgcttc aggaaccata c
//
LOCUS       DAOPUR010000405         1290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_121756_length_1290_cov_2.760324, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000405 DAOPUR010000000
VERSION     DAOPUR010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121756_length_1290_cov_2.760324"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKN90_06715"
     CDS             complement(<1..411)
                     /locus_tag="PKN90_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86003.1"
                     /translation="MKIHTLNSNSWVSWYEIEVYATPAKPTISQEGELLTSSFTTNNQ
                     WYFNGNPIQDAIYQTYTATLPGSYQVSVSYGDECESMSEILALTTTGVNENSTNDVRI
                     YPNPSKDNILVEGVKNGILEIINLQGKIVKYTPIP"
     assembly_gap    462..471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<472..826)
                     /locus_tag="PKN90_06720"
     CDS             complement(<472..826)
                     /locus_tag="PKN90_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNZ86004.1"
                     /translation="MKRINKLIMFSAIIACCINTVNAENKCSSNIALNKPATASSSTE
                     TEFPIHAVDGNLSTNWCTPGFNGWITVDLQNTFSIDSIKLYVNQYIAGNTEHEIKISD
                     DMDNWILAKTLSGETY"
BASE COUNT          385 a          193 c          208 g          494 t
ORIGIN      
        1 aggtattggg gtatatttaa ctattttccc ttgtaagttt attatttcca atatcccatt
       61 tttcactcct tccacaagaa tattgtcttt gctaggattt ggataaatac gtacatcatt
      121 tgtgctattt tcgtttacac cagttgttgt taaagctagt atttcagaca ttgattcaca
      181 ttcatcgcca tagctaacac tcacctgata ggaaccagga agcgtggcag tatatgtttg
      241 ataaatagca tcttgtattg gattgccgtt aaaataccat tgattgttgg tagtgaaact
      301 tgaggttaat agttcacctt cttgagaaat ggtaggttta gcaggggttg catatacctc
      361 aatttcatac caagataccc atgagtttga atttagagta tgtattttca cacctctaac
      421 atttaataag gcaggatcaa atttaatagt aagtgtctga tnnnnnnnnn ntataggttt
      481 ctcctgatag ggttttggct aatatccaat tatccatatc atcagatatt tttatctcgt
      541 gttcagtatt gcctgcaata tattgattta cgtataattt tatactatct attgagaatg
      601 tgttttgtaa atcgaccgta atccaaccat taaatccagg agtgcaccaa ttggtactta
      661 aattaccatc aactgcgtga attggaaatt ctgtttcggt tgacgaactg gcagttgcag
      721 gtttatttaa ggcaatattt gatgagcatt tattttcagc attaacagta tttatacaac
      781 aagctattat cgctgagaac atgattaatt tattgattct tttcataaat attgatttta
      841 taaatagtaa atttattatg agtttattcg aatttctttg taacaactat gcgattctac
      901 ttctacatac aagctaaaaa atattgttac ctgtatgaga aactgttcgt tggtagttaa
      961 taatagattt acaaaactat aaggtattta agctctagtt tttgcaattt tttccgtgtt
     1021 ataaaattat caatttacta tgctctctct ctcttaaagc tttagttgaa ttcttgtttc
     1081 aaatataaaa tgtttttgga taatcgcaaa atatattgca atttattttt agtattggag
     1141 ttttgttcta caaacgacta gccataaacc cctcgttgtg aaaacgaggg gtagggattt
     1201 cttatgtttt tttttgtttg gttcgaatat ggaaggctgg agctttcctt ttaataccaa
     1261 ctgtacatat aaaataaccc aaaattattt
//
LOCUS       DAOPUR010000406         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_121890_length_1289_cov_2.793355, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000406 DAOPUR010000000
VERSION     DAOPUR010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121890_length_1289_cov_2.793355"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKN90_06725"
     CDS             complement(<1..325)
                     /locus_tag="PKN90_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNZ86005.1"
                     /translation="MEPIVLAYIGIGLMVGLAGTGSAFGCSIAGSASVGAMKKNADGF
                     GSYLLLTALPGSQGLYGFAGYFLMSNFFGLLTTDITWLQAGAVFGSGLAVGLVGLLSA
                     IRQGQV"
     gene            complement(358..>1289)
                     /locus_tag="PKN90_06730"
     CDS             complement(358..>1289)
                     /locus_tag="PKN90_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNZ86006.1"
                     /translation="VFEFIGSLYELPNHKELDLTPFFAPFYMLFFGFALGDAGYGLLI
                     VIAATVLKFKKPAMKSILTLAQFLGISTVIFGALTGTLFGINLMNVDIAWLQNAKQYM
                     INTDKMFTLALALGTIQILFGMVLKVVNLSMAKGFAYAYSTIGWLILIIGLGANYFLT
                     QLQAYTPQSSTYIQYGVLSISGALILLFNNPKRNILMNFLAGLWDVYGMLTGLIGDLL
                     SYIRLFALGVSSAILGFVFNELAMSLRPDNIILGPIVMIIILVLGHGMTIFMSALGAF
                     VHPIRLTFVEFYKNAGFVGGGKAYKPFAEKKEN"
BASE COUNT          453 a          276 c          183 g          377 t
ORIGIN      
        1 atacttgacc ttggcgtata gctgataaaa gacccactaa acctactgcc aaacctgacc
       61 caaatacagc acctgcttgc aaccatgtaa tgtctgttgt aagtaatcca aagaaattac
      121 tcatcaagaa atatcctgca aagccatata atccctgact accaggcaat gcagttaata
      181 ataaatacga accaaatccg tccgcatttt ttttcattgc ccccacagag gcactacctg
      241 ctattgaaca gccaaacgca cttcctgtgc cagctaatcc aaccattaat ccaattccga
      301 tgtaagctaa aacaataggt tccatttttt ttcttttttt aattgttgtt attcttatta
      361 attttctttt ttctcggcaa acggtttgta ggcttttcct ccacctacaa atcctgcatt
      421 tttataaaat tctacaaatg ttaatcgaat aggatgtaca aaagcaccca atgcagacat
      481 aaaaattgtc attccgtgac ctaataccaa gataattatc attactatgg gacctaatat
      541 gatattatct ggacgtaaac tcatagccaa ttcattaaac acaaatccta aaatagcact
      601 agataccccc aatgcaaata gtcgtatata ggataataag tcgccaatca agcctgtaag
      661 catgccatat acatcccaca acccagctaa aaagttcatt aaaatattcc gtttaggatt
      721 gttgaataat aaaatcaagg ctccagaaat gcttaatact ccatattgta tgtatgttga
      781 cgattggggt gtgtaagcct gtaattgggt taaaaaataa tttgcaccca aaccaataat
      841 tagtatcaac catccaatag tagagtaggc ataagcaaaa ccttttgcca tcgacaaatt
      901 taccactttt agcaccatgc caaagagaat ttgaatagtt cctaaagcca atgctaaagt
      961 gaacattttg tccgtattta tcatgtattg cttcgcattt tgtaaccatg caatatctac
     1021 gttcattaga ttgattccaa acaatgttcc agtaagtgct ccgaaaataa ccgtagaaat
     1081 gcctaagaac tgtgcaagag taagaatgct tttcatagca ggtttcttaa actttagtac
     1141 tgtagcggct attactatca ataatccata acccgcatca cccaaagcaa aaccaaaaaa
     1201 caacatataa aatggagcaa agaaaggcgt taaatctaat tctttatgat tgggtaattc
     1261 gtacaaactg ccaataaact caaatactt
//
LOCUS       DAOPUR010000407         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_122000_length_1288_cov_4.203569, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000407 DAOPUR010000000
VERSION     DAOPUR010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122000_length_1288_cov_4.203569"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /gene="dtd"
                     /locus_tag="PKN90_06735"
     CDS             complement(<1..396)
                     /gene="dtd"
                     /locus_tag="PKN90_06735"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNZ86007.1"
                     /translation="MRAVIQRVLKASVTINKEVHSEISQGLLLFVGIEDLDTEQDIDW
                     LVAKIVNLRIFSDDKGLMNKSVLDYGGDILVVSQFTLFAQTKKGNRPSFIRASKPDIA
                     IPMYQLFCQKLSNCLGKDVKEGIFGADMQV"
     gene            622..>1288
                     /locus_tag="PKN90_06740"
     CDS             622..>1288
                     /locus_tag="PKN90_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNZ86008.1"
                     /translation="MKHLFVGAFLLYVVSIFAQPPINYYQPAYNKSGTQLRTALQGII
                     DNHTVVSYNGLYDVYETSDNTADNKVWDMYSYCSWTHNNKTCGNYTKVCDCYNREHSV
                     PQSWFNKKSPMVSDAFHVYPTDGRVNGQRDNDPFGECSGGTTLDSNAKGKSGTSTFSG
                     YSGTVFEPVDEYKGDFARTYFYMATRYAKVEPEPNPLKPLPRCEEWGNNVFSYDNLGL
                     TNYA"
BASE COUNT          427 a          228 c          222 g          411 t
ORIGIN      
        1 cacctgcata tctgctccaa atataccttc ttttacatcc ttacccaaac aattactcaa
       61 tttctggcaa aaaagttgat acatcggtat agcgatatca ggtttagagg ctctaataaa
      121 cgatggtcta tttccttttt ttgtttgagc aaatagcgta aactggctta ccaccaatat
      181 gtctccacca tagtctagca cagatttatt catcaatccc ttgtcgtcag aaaatatacg
      241 taggtttaca atcttagcca ctaaccaatc aatatcttgc tctgtatcca aatcttctat
      301 accaacaaat agcaacaaac cctgagatat ttcagaatgt acctctttat tgatggtaac
      361 agatgccttt aaaacacgtt gtataacagc cctcattgta tataattttc ttcacaaaat
      421 tacaatttat acgatactta atcaaatatt aataagttaa ttcattgata ttttttgttt
      481 ttacaaattt aaacaagtac atttgtgagt aatcagtaca cgcgtaacag gtttgatacg
      541 tgctgtaaat atgtaaaaag gaatcatttt ctgataaaat ggtgatatga tgcatctaaa
      601 caagttgtaa ttttaaatgt tatgaaacat ctatttgtag gagcattctt actgtatgta
      661 gtaagtatat ttgcccaacc gcctatcaat tattaccaac ctgcatataa taaaagtgga
      721 acgcaattgc gtacagcctt acaaggtatt attgataatc atacggttgt gtcatataat
      781 ggattgtatg atgtgtacga aacaagcgat aatactgccg acaacaaagt gtgggatatg
      841 tattcatatt gcagctggac acacaacaat aaaacgtgtg gaaattacac caaagtatgt
      901 gattgttaca atcgcgaaca cagtgttcca caaagttggt ttaacaaaaa atcgccaatg
      961 gtatccgatg cttttcatgt ttacccgaca gatggcaggg taaatggaca acgtgataat
     1021 gatccttttg gagaatgttc tggtggcaca acacttgata gtaatgctaa aggaaaatcg
     1081 ggaacttcaa ctttttcagg atattcgggt acagtgtttg agcccgtaga tgaatataag
     1141 ggtgattttg ctcgtaccta tttttatatg gctacacgct atgctaaagt agaacctgaa
     1201 cctaatcctt taaagccttt gccaagatgt gaagagtggg gaaacaatgt gtttagttat
     1261 gacaatttag gtttaacgaa ttacgcag
//
LOCUS       DAOPUR010000408         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_123659_length_1277_cov_3.871522, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000408 DAOPUR010000000
VERSION     DAOPUR010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123659_length_1277_cov_3.871522"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PKN90_06745"
     CDS             complement(<1..896)
                     /locus_tag="PKN90_06745"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HNZ86009.1"
                     /translation="MKNVVLLILTSLLLLTGCDEKSLKPTITGSAFEILLVVDEPIAA
                     SEAGKTLFNILNQDMYGLPQSEPLFKVSRTPHSGFTAILRPARNIIQLEINDTYSRTR
                     IVTQNDVWAAPQAVIKITSNSVEAAQQAIEKYQQDILDFFVTAERDRTIAYQKRYAEK
                     DAMKKVQDKFGISIVLPKGMNRFKEEENFLWISNASDDVRQNIVIYTYPYTSKETFTR
                     ENLLHKRDSVMLLWVPGPNPDSYMATEYRVDPPILNEIAINGTYAAELRGMWRVQGDL
                     MGGPFVNITRLDEKNQRVVTVEA"
     gene            complement(906..>1277)
                     /locus_tag="PKN90_06750"
     CDS             complement(906..>1277)
                     /locus_tag="PKN90_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNZ86010.1"
                     /translation="RKELVPLEKTQPMQEIMALVKNYDFTHQRRLSIEYILFDQLNDT
                     EEQALLLASMLKQIPCRINLIPYHRIPQVGLMPSSREKIENFQKILMKKGITTTIRRS
                     RGQDIEAACGMLSTKFVNTSN"
BASE COUNT          335 a          245 c          259 g          438 t
ORIGIN      
        1 gcttctacgg ttactacacg ttgatttttt tcgtctaatc gggtaatatt tacaaacgga
       61 ccacccatta aatctccctg tacacgccac ataccacgta gttcggctgc gtatgtaccg
      121 ttaattgcta tttcgttcag tatgggagga tctactcggt attcggtagc catatacgaa
      181 tctggatttg gtccaggcac ccacaagagc atgacagaat cgcgtttgtg taacagattt
      241 tcacgcgtaa atgtttcttt gctggtataa gggtaggtgt agataacaat attttgacga
      301 acatcgtcgc tagcattgct tatccacaaa aagttttctt cttctttaaa acggttcata
      361 cctttgggta atacgatgct aatgccaaat ttatcttgca cttttttcat cgcatctttt
      421 tcagcatagc gtttttggta tgctatagtg cggtctcttt cagctgttac gaaaaaatcg
      481 agaatgtctt gttggtattt ctcaatggct tgctgtgcag cttctaccga gttactcgta
      541 atttttatta ccgcttgcgg agcagcccat acgtcgtttt gagttacaat tcgtgtgcgt
      601 gaatacgtat cgtttatttc caattgaatg atgtttcgag ctggacgcaa gatggcagta
      661 aacccactat gcggggtgcg agacacttta aataaaggtt cggattgagg caagccatac
      721 atatcttgat tgagaatatt aaaaagcgtt tttccagctt cgctagcagc tatcggttcg
      781 tctaccacca gcaaaatttc aaaagcactt cctgtaatag ttggttttag gcttttttcg
      841 tcgcatcctg tcagtaataa cagggaagta agtataagaa gaaccacgtt tttcataggc
      901 tataattagt ttgaagtatt cacaaatttt gttgataaca taccgcaagc agcctcaatg
      961 tcttgtccac gtgagcgtct tatggtggta gtaatgcctt ttttcatcag tattttttga
     1021 aaattttcga ttttttcgcg actagaaggc attaatccaa cctgtggaat acgatgatat
     1081 ggaataagat tgattctgca cggaatttgt ttaagcatac ttgctaataa cagagcttgc
     1141 tcttcggtat cgtttagttg atcgaacaaa atatattcga ttgacaaacg tctttggtgt
     1201 gtaaaatcat aattttttac caaagccatt atttcttgca tgggttgtgt tttttcgagt
     1261 ggcaccaatt ccttgcg
//
LOCUS       DAOPUR010000409         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_123964_length_1275_cov_3.266393, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000409 DAOPUR010000000
VERSION     DAOPUR010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123964_length_1275_cov_3.266393"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /gene="guaA"
                     /locus_tag="PKN90_06755"
     CDS             <1..830
                     /gene="guaA"
                     /locus_tag="PKN90_06755"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNZ86011.1"
                     /translation="LNKAIGKNLTCIFVDHGLLRKNEFETVLDDYAHLGLNVIGVNVK
                     DRFYAKLAGVTDPEQKRKIIGKEFIEVFDEEAHKIQDVKWLAQGTIYPDCIESLSITG
                     TVIKSHHNVGGLPEKMNLKLCEPLRLLFKDEVRKVGNELGMMSHLIKRQPFPGPGLAV
                     RILGDITAEKVRILQEADDIFIKGLREWNLYDQVWQAGVILLPVQSVGVMGDERTYEN
                     AVALRAVTSTDAMTADWAHLPYEFLGKMSNEIINKVKGVNRVVYDISSKPPATIEWE"
     gene            920..>1275
                     /locus_tag="PKN90_06760"
     CDS             920..>1275
                     /locus_tag="PKN90_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNZ86012.1"
                     /translation="MKSKLFIIGVLVNLFIGFAGIAYAQQSANNSNSTSSEVSAMAFE
                     KTVHDFGTFNEADGKVETVFVFKNTSKENLVVSRVQASCGCTTPDWSKEPIAPGKKGF
                     VKASYNPAGRPGNFNK"
BASE COUNT          427 a          192 c          266 g          390 t
ORIGIN      
        1 tgttaaacaa agccattggc aaaaacttaa cgtgtatttt tgtagatcac ggtttgttgc
       61 gtaaaaatga attcgaaaca gtattagatg attatgctca tttaggtctc aacgtaattg
      121 gggtaaatgt aaaagaccgt ttttatgcaa aattagctgg tgtaactgat ccagagcaaa
      181 aacgtaaaat aattggtaaa gaatttatag aggtatttga tgaagaagct cataaaatac
      241 aagacgtaaa atggttggct caaggcacta tttatcctga ctgtatcgag tctctttcta
      301 tcacaggcac agttattaaa tcgcaccata acgtaggtgg gttaccagag aaaatgaact
      361 tgaaattgtg cgaaccatta cgtttacttt tcaaagatga ggtgcgtaaa gtaggtaatg
      421 agctaggaat gatgtcgcac cttattaagc gtcaaccatt tccaggtcct ggtttggctg
      481 tacgtatatt aggtgatatt actgccgaaa aagtacgcat attgcaagaa gctgatgata
      541 tttttatcaa aggattgcgt gaatggaatc tttacgacca agtatggcaa gctggtgtta
      601 tcttattgcc tgtgcaatct gttggtgtaa tgggcgatga acgtacatac gaaaatgcgg
      661 tagccttgcg tgctgtaaca tctactgatg ctatgacagc cgattgggca cacttgcctt
      721 acgagttttt aggcaaaatg tcaaacgaaa tcatcaataa agtaaaagga gtaaatagag
      781 tagtgtatga tattagctcg aaaccacccg caactattga atgggagtaa tataagttta
      841 aaaaaaatta aaaaaagaac atttctttgt ttttttacat agctttgtgg cagtaaataa
      901 taacaaaaaa tatacaacta tgaaatcgaa actttttatc attggcgttt tagttaatct
      961 ttttattggt tttgcaggta tagcctatgc tcaacaaagt gcaaataatt ctaattcaac
     1021 ctcatctgag gtgtcggcga tggcgtttga aaagaccgtt catgattttg gaacttttaa
     1081 tgaggcagat ggtaaagttg aaactgtatt tgtatttaaa aatacaagca aagagaatct
     1141 tgtagttagt agagtgcaag cttcttgtgg atgtactact cccgattggt caaaagaacc
     1201 tattgcccca ggaaaaaaag gttttgttaa agcatcatat aatccagctg gtcgacctgg
     1261 taattttaac aaaag
//
LOCUS       DAOPUR010000410         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_125552_length_1265_cov_3.305785, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000410 DAOPUR010000000
VERSION     DAOPUR010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125552_length_1265_cov_3.305785"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKN90_06765"
     CDS             complement(<1..352)
                     /locus_tag="PKN90_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86013.1"
                     /translation="MAENFTPYKIKLQGIGGYIHPHDENVEVLVHGPALGGEIALELG
                     TDGSESWHIHHNFPDIGIAAQYLTLSNPEILGEMISLYPYINFPMVVTDWFSFYLKLG
                     GGVALATKPTDIEGA"
     gene            514..1242
                     /gene="gldF"
                     /locus_tag="PKN90_06770"
     CDS             514..1242
                     /gene="gldF"
                     /locus_tag="PKN90_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HNZ86014.1"
                     /translation="MYVLFIKEIRSFFSSAIGFIVIGIFLLLMGTFLWLLPGENNVLE
                     SGYANVDGLFLLAPWLYLFLIPALTMRFFAEEKKSGTIELLLTRPLSTLQIVLAKYFA
                     GVVLVVLSLLPTLVYVYSVYQLGLPQGNIDMGGFLGSFIGLIFLGAGYTAIGLWASSV
                     SDNQIVSFLVAALLSFLLYYGFELLSLFFTSAYVQDMVSQMGMSYHYDSMSRGVIDSR
                     DVLYFVTISVVFLFATTWFVTKRK"
BASE COUNT          327 a          203 c          269 g          466 t
ORIGIN      
        1 tggcaccttc tatatctgtg ggtttggtag ctagggctac accgccccct aatttgaggt
       61 aaaacgaaaa ccaatcggta actaccattg ggaagttgat atacggatat aggcttatca
      121 tttcgcctaa gatttctgga ttactcagcg taaggtattg agctgcaatg ccaatatcag
      181 gaaaattgtg gtgaatatgc caactttcgg agccatcagt gcctagttcg agggcaattt
      241 cgccacctaa agctggtcca tgcacaagta cctccacatt ttcatcgtgt ggatgaatgt
      301 agccacctat accttgtaat ttaattttat acggagtaaa attttccgcc attatacaac
      361 tacagaatgt ataaaatagt aggagtatac atggtgcttt ttttatcatt gttcgtgttt
      421 tgagttccca tacaaaatgc gtatctttgt gcttgttcgc aataagcgaa attcgattac
      481 gcaaatttac atattttata ctaaaaacaa gctatgtacg tattgtttat taaagaaatc
      541 cgttcatttt tttcatccgc tatcggcttt attgtgatag gtattttttt attgcttatg
      601 ggcacattcc tatggttact acctggcgaa aacaatgtgc ttgaaagtgg ttatgccaat
      661 gttgatggat tgtttttgtt ggctccatgg ttgtatttat ttttgatacc agcacttaca
      721 atgcgttttt ttgcagaaga aaaaaaatcg ggtactatcg aattgttact tacacgaccg
      781 ctcagtactt tgcagattgt attagccaaa tattttgcag gcgtagtatt ggttgtgttg
      841 tctttgctac ctaccttggt atatgtgtat agtgtgtatc agttgggttt gccacaaggc
      901 aatatcgata tgggtggatt tttgggttct tttattggac ttattttttt aggagctggt
      961 tatacggcta ttgggttatg ggcttcgtct gttagtgata accagattgt atcttttctt
     1021 gtggcagctt tgttgtcatt tttattgtat tatggttttg aattactatc tctatttttt
     1081 acttcggcat atgtgcaaga tatggtatcg caaatgggca tgagctacca ttacgattca
     1141 atgagtcgtg gtgtaattga tagtagagat gtcttgtatt ttgttaccat cagtgttgtt
     1201 tttctgtttg ctactacttg gtttgtgact aaaagaaaat agcaagggag tgaaaaagga
     1261 atgca
//
LOCUS       DAOPUR010000411         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_126727_length_1258_cov_2.683292, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000411 DAOPUR010000000
VERSION     DAOPUR010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126727_length_1258_cov_2.683292"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKN90_06775"
     CDS             <1..363
                     /locus_tag="PKN90_06775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ86015.1"
                     /translation="KTFAASYNNQLIGVRMELCYKDCVYDWYAGADDNYRNHYPNDFV
                     PYHILMWGQNNGYKYFDFGGAGKPNVPYGVREHKLKFGGTLVNYGRFEKIHKPFLMLL
                     GKFGFFLYKFVKKTGKKG"
     gene            360..>1258
                     /locus_tag="PKN90_06780"
     CDS             360..>1258
                     /locus_tag="PKN90_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ86016.1"
                     /translation="MIPKINAKYSLMNTIKGIVYALIGKQKYGLHEENNTYCVNHART
                     GLRMALSALQLPANSKVGVGMYNCYTVMNAIKIAGYQPVFIDVTTDFVLDLKDLESKK
                     NQLSALIVTHLFGIPNDMTQIAAICTGMPIIEDCAHAFLSKDSQQQALGTKGDFAIYS
                     IGQGKFPSIGDGGLLQVNNQSYLPYIEQEYTNLFSYSKIKDALSCVAAFSKSILHKPF
                     IYRFILPIKKRSQDKKLSTTTYGHVEKKMATSVYKRYREEQPYYLTYLQKQQDNTREI
                     LQHVQHIADCYIPFMDTNNYNCF"
BASE COUNT          461 a          189 c          218 g          390 t
ORIGIN      
        1 aaaacatttg cagctagcta taacaatcaa ttaattggag ttagaatgga attgtgttac
       61 aaagactgtg tgtacgattg gtatgcagga gctgatgata attaccgcaa tcattatccc
      121 aatgattttg taccctatca tattttaatg tggggacaaa acaatggtta taagtatttt
      181 gattttggag gagcaggaaa accaaatgtt ccttatggtg tacgcgaaca taaactaaaa
      241 tttggtggca cattggtaaa ttatggacgt tttgaaaaaa ttcacaaacc ttttttaatg
      301 ttactaggta aatttggatt ttttctttat aaatttgtaa aaaaaacggg gaaaaaagga
      361 tgataccaaa aattaatgca aaatattcat tgatgaatac cattaaaggt attgtttatg
      421 cacttatcgg caaacaaaaa tatggattgc atgaggagaa taacacgtat tgtgtcaatc
      481 atgcacgcac aggtcttaga atggcgttat ccgctttgca attgcccgct aatagcaaag
      541 taggtgtagg tatgtacaat tgttatacag taatgaatgc cattaagata gctggttatc
      601 aaccagtatt tattgatgtt acaacagact ttgtgcttga tttaaaagac cttgaaagca
      661 aaaagaacca gttatcagct cttattgtaa ctcatttatt cggaattccg aatgatatga
      721 cgcaaatagc agcaatatgc actggtatgc caattattga agattgtgca catgcttttt
      781 tatctaaaga tagccaacaa caagctctgg gaacaaaggg tgattttgct atttattcaa
      841 ttggacaggg aaaatttcct tcaatcggag atggaggtct tctacaggta aataatcaat
      901 cctatttacc atatatagag caagaataca ccaatttatt tagctactca aaaataaaag
      961 acgcattatc ttgtgtagca gcatttagta aatctatatt acataaacca ttcatttaca
     1021 ggtttatatt accaataaaa aaacgaagtc aggataaaaa attatctaca accacctatg
     1081 gtcatgtaga aaagaaaatg gcaacatctg tatataaacg ttatagggaa gaacaaccct
     1141 attaccttac ctatttacaa aagcaacaag ataatacaag ggaaatactg caacatgtac
     1201 aacacatcgc tgattgttat attcctttta tggatacaaa taattataat tgttttat
//
LOCUS       DAOPUR010000412         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_128094_length_1249_cov_3.639028, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000412 DAOPUR010000000
VERSION     DAOPUR010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128094_length_1249_cov_3.639028"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PKN90_06785"
     CDS             <1..587
                     /locus_tag="PKN90_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNZ86017.1"
                     /translation="LGAFAFAISGIRLASKKQFDWFGAYIVGLVTAIGGGTLRDLLLD
                     QPPFWMEQGWYLISTLMALLFVVALGKYVNKLSSSFFVFDTIGLAVFTIAGIEKTLVF
                     GYSYWIAIVMGVMTGCLGGIIRDIFINETPLIFRKEIYAIASIIGGILYLVSLHVFNL
                     SELISYVIGFSSIVLVRFLAVKYEISLPQLRDSQ"
     gene            592..>1249
                     /locus_tag="PKN90_06790"
     CDS             592..>1249
                     /locus_tag="PKN90_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ86018.1"
                     /translation="MSTQIKFCSLASGSSGNCYYVGNEKHGILIDAGIGVRTIKKRLK
                     DIGVALESILGIFVTHDHFDHIASIGVLGEIHHIPIFSTSEILDGINRCYKVTQKLQH
                     SKRPIAKNKIIEIGSFTIEAFSVSHDASDCVGYTICQGSKKFTIATDLGYINTEAAQH
                     LMNATSFVVESNYDEQTLLENPSYPTYLKERILSNKGHLSNHALAEFLSINYQEHWQH
                     I"
BASE COUNT          397 a          208 c          237 g          407 t
ORIGIN      
        1 ttttaggagc ttttgcgttt gcaattagcg gtatccgttt ggcttcaaaa aaacaatttg
       61 attggtttgg tgcgtatatt gtaggcttag taactgccat tggaggagga accttacgcg
      121 acctattact agatcaacct cctttttgga tggaacaagg atggtacctc atcagtactc
      181 tcatggcttt actttttgtg gttgcattag gcaaatatgt aaataaatta tcttcttcgt
      241 tttttgtatt tgatacaata ggcttagctg tatttacaat tgctggtatc gaaaaaacat
      301 tagtatttgg ctattcttat tggatagcta ttgtaatggg agtaatgaca ggatgcttag
      361 gtggaattat tagagatatt ttcataaacg aaacaccctt aattttcaga aaagagattt
      421 atgctatcgc atctattata ggaggtattt tgtatttagt atccctacat gtgtttaacc
      481 taagtgaatt aatctcgtac gttataggtt ttagtagcat tgtcttagtg cgatttttag
      541 ccgttaaata cgaaatcagc ttaccccaat tacgtgattc tcaataagta tatgtctact
      601 caaattaaat tttgtagttt agctagcgga agtagtggaa actgttatta cgttggaaac
      661 gaaaaacatg gcattttgat tgatgctggt attggagtta gaacaataaa aaaaaggttg
      721 aaggatatag gcgttgcgtt agaatcaata ctagggatat tcgtaacgca cgaccacttt
      781 gatcatattg catctatagg tgtactggga gagattcacc acattcccat tttttcaacc
      841 tctgaaatat tagacggcat aaatcgttgc tacaaggtaa cgcaaaaact tcaacatagc
      901 aaacgaccca ttgcaaaaaa taaaatcatt gaaattggaa gtttcaccat tgaggctttc
      961 tctgtatcac atgatgcctc tgactgtgtg gggtatacca tttgccaagg ctcaaaaaaa
     1021 tttacaatag ctaccgactt ggggtatatc aatactgaag cagctcaaca cttaatgaat
     1081 gccacttcgt ttgtggtaga atcaaattat gacgaacaaa ccctattgga aaatccatcc
     1141 tatccaacat atctaaaaga gcgtattttg agcaacaaag gacatttgag taaccacgca
     1201 ttagcagaat tcttatcaat taattaccaa gaacattggc aacacattt
//
LOCUS       DAOPUR010000413         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_130039_length_1237_cov_3.181895, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000413 DAOPUR010000000
VERSION     DAOPUR010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130039_length_1237_cov_3.181895"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PKN90_06795"
     CDS             complement(<1..1206)
                     /locus_tag="PKN90_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ86019.1"
                     /translation="MKPGKNGVWQAQVTAKNLGVKSLDGLYYQYEVYAYGKSRLALDP
                     YAKSMAAHAPKGTDKIGKAAIVDMKSPRATPANFVRKYTNYDHIANDIDLVCYEVHVR
                     DFTVQPGVVKPELAGTYKGFAEKTDYLKELGITHAQLLPIQNFFTTDEKNRQFSDTDA
                     PVSNYNWGYDPHNYFTPEGWFSSNAADPYARIRELRQLVQDLHNKGIGVIMDVVYNHT
                     YLVETFENVAPGCYYRYNDTNGISAHSGAGPTVESRRKMVQKLTVESLNHFVTEYLVD
                     GFRFDLMGFIDHETMRLIRQEVGKTYNPNNINELVLQGEAWVFSDLDTDAKATGIHAA
                     TTKLNHPKEYLNLGFFNDVSRDAFAGREHQKGFVQGSYGEIDRCVTAILGGVKNFPMT
                     GKAFATEVLT"
BASE COUNT          303 a          250 c          281 g          403 t
ORIGIN      
        1 ggtaagtact tcggtggcaa acgctttacc tgtcatcggg aagtttttta cacctcccaa
       61 aatggctgta acgcaacggt caatttcgcc atagcttcct tgaacaaagc ccttttgatg
      121 ttcacgtcca gcgaaagcat cgcgcgatac atcgttaaaa aagcccagat ttaggtattc
      181 ttttgggtgg tttagtttgg tggtagcggc atgtatgcca gtagctttgg catcggtatc
      241 taaatcggag aatacccacg cttcgccttg caataccaat tcgttgatgt tgttcgggtt
      301 gtatgtttta cctacttctt ggcggataag acgcattgtt tcgtggtcaa taaagcccat
      361 taggtcgaaa cggaaaccat caaccaagta ttcggtaaca aaatgattga gtgattctac
      421 ggttaatttt tgtaccattt tgcgacgcga ttctactgta ggtcctgcac ccgaatgtgc
      481 cgaaatacca ttggtatcat tgtaacggta atagcaacca ggagctacat tttcgaatgt
      541 ttctaccaaa taggtatgat tataaactac gtccataatc acaccaatac ctttgttgtg
      601 taagtcttgt actaattgac gtaactcacg aatacgtgca taggggtcgg cagcgttgct
      661 cgagaaccat ccttctggcg taaagtagtt gtgagggtcg tatccccagt tgtagttgct
      721 tacaggagca tctgtgtcag aaaactgacg gtttttttcg tctgtagtaa agaaattttg
      781 aataggcaat agttgagcat gcgtaatgcc taattctttt aaataatctg ttttctcggc
      841 aaatcctttg tacgttccag caagttcggg ttttaccact cctggttgta cggtaaagtc
      901 gcgtacatgt acttcgtagc acactaggtc aatgtcgttg gctatgtggt cgtagtttgt
      961 atatttacgt acaaaatttg cgggtgttgc acgaggcgat ttcatatcta cgatggctgc
     1021 tttgcctatt ttgtccgtac ctttgggagc atgtgcagcc attgattttg cgtatgggtc
     1081 gagggctaaa cgtgatttgc cataagcata cacttcgtat tggtaatata atccatctaa
     1141 cgatttaacg ccaaggtttt tggctgttac ttgagcttgc caaacaccat ttttgccggg
     1201 cttcatctca attttgcgtt tgatgggttg ttcgttt
//
LOCUS       DAOPUR010000414         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_130232_length_1236_cov_3.424217, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000414 DAOPUR010000000
VERSION     DAOPUR010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130232_length_1236_cov_3.424217"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..482
                     /locus_tag="PKN90_06800"
     CDS             39..482
                     /locus_tag="PKN90_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86020.1"
                     /translation="MIFLLAAIKKMGGKVRYVYSPKAIVDIYPSPSLSDFLQQRMRWL
                     SKSASYSDSDVIFVSFTVLFAQIAIVIAGALAIFNMMFVPIFISLFFSKWVVDSWLLY
                     KASDFLGQKRLVLYSLLLSLVYPFYVISVACCSMICPIKWKNRNI"
     gene            493..>1236
                     /locus_tag="PKN90_06805"
     CDS             493..>1236
                     /locus_tag="PKN90_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNZ86021.1"
                     /translation="MKSVVYLALFSLLLFVSCQSNEQSTSSQKQETQDLIQNPFYNAD
                     SAYVFVANQVAFGPRVPNTDAHKKCGDYMVATLQRFGAEVTEQRVQLKAYNNTILDAR
                     NIIGSFNPEQKTRIFLCAHWDSRPYSDNDTDSANYHTPVQGANDGASGVGVLLELARL
                     MQQVPPTIGVDIILFDAEDYGRPSFDKSADIGDSFCLGSQYWSRNPHKEGYKPKYGIL
                     LDMVGGKNPTFLQEYFSMLYAPDVMKMVWK"
BASE COUNT          363 a          205 c          245 g          423 t
ORIGIN      
        1 tgtacaaaat gagttggtag atacagaagt ttcgggagat gatatttttg ttagctgcaa
       61 ttaaaaaaat gggaggcaag gtgcgttatg tgtattcccc aaaggcgatt gttgatatat
      121 atccttctcc atccctttcg gattttctac aacaacgtat gcgttggctc tcaaaatcag
      181 catcctattc agatagtgat gtcatttttg tttcttttac agtactattt gctcaaatag
      241 caatcgttat tgctggtgct ttagctattt ttaatatgat gtttgtgcca atttttatat
      301 ctttgttctt tagtaaatgg gttgtagata gttggttact ctataaggcg tctgattttt
      361 taggacaaaa aagattagtt ttatatagtt tacttctctc gttagtctat cctttttatg
      421 ttatatcggt ggcttgttgt tctatgattt gccccataaa atggaaaaat cgaaacattt
      481 aattataatt ctatgaaatc agtagtatac ttagcattat tttctttgct tttatttgta
      541 tcatgtcaat cgaacgaaca gtctacctca tcgcaaaagc aagagacaca agacctcatc
      601 caaaatccat tttataatgc ggattctgcc tatgtatttg tagccaacca agtggcattt
      661 ggacctagag tacccaatac agatgcacat aaaaagtgtg gagattatat ggttgccact
      721 ttacagcgtt ttggggcaga agtaacagag caaagggtgc aattaaaagc atacaacaat
      781 acaatactag atgcacgtaa tattattggg tcatttaatc cagaacaaaa aacgcgtatc
      841 tttttatgtg cacattggga ctctcgtccg tattctgata atgatacaga ttctgctaac
      901 taccatacgc ctgtgcaagg agcaaatgat ggagcgagtg gtgtaggcgt attgcttgaa
      961 ctagctcgtt taatgcaaca agtacctcca acaattggtg tagatattat tctttttgat
     1021 gcagaagatt atggtagacc ctcattcgat aagtctgccg atataggcga ttcattctgt
     1081 ttgggctctc aatattggtc acgtaatccg cataaagaag gctataaacc taaatatggt
     1141 attttactgg atatggtagg gggtaaaaat cctactttct tgcaggaata tttttcgatg
     1201 ctctatgctc cagatgtgat gaagatggtg tggaaa
//
LOCUS       DAOPUR010000415         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_130260_length_1236_cov_2.884843, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000415 DAOPUR010000000
VERSION     DAOPUR010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130260_length_1236_cov_2.884843"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKN90_06810"
     CDS             complement(<1..240)
                     /locus_tag="PKN90_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HNZ86022.1"
                     /translation="MPVTLHHIGIWVDNLDKMRQFYEHYFSAHCSKKYINPITNFSSY
                     FLSFGNDSTRIELMCRVDTTIEPTKRGIMKGYAHIA"
     gene            complement(252..1211)
                     /locus_tag="PKN90_06815"
     CDS             complement(252..1211)
                     /locus_tag="PKN90_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNZ86023.1"
                     /translation="MPVLLNIRTIFRNLSFCQQDITDFKPDEVILIDYPGFNLKIAKF
                     VKNHLKLPVFYYIPPKIWAWKEYRIKAIKKYIDKVFTIFPFETEFYAKHQCEVQYVGN
                     PSVESVHTYQATAPNRSIFIQQNALDDKKIIALLPGSRKQELEACLPIMLQVDFSQFA
                     DYQFIVAGTDALPCTLYHTLMKGSNAKIVYGKTYDLLQHAEAAIVTSGTATLETALFN
                     VPQVVCYEAFLSRLVAPFLRMVLKIKYVSLVNIIAQKELVKELLIQDFTPKNTQKELK
                     KLVFDQAYRANILAGYQQLHTELGTTIASDTAAKAMVNYLKKE"
BASE COUNT          393 a          221 c          202 g          420 t
ORIGIN      
        1 tgctatgtgt gcataacctt tcattatgcc tctttttgtg ggttcaatgg ttgtatcaac
       61 cctacacatt aactctatcc tagtactatc attaccaaac gacaaaaaat aggaactgaa
      121 attcgttatg ggattgatat attttttgct acaatgagca ctaaaataat gttcatagaa
      181 ttgacgcatt ttgtccaagt tatcaaccca aatccctata tgatggagtg taactggcat
      241 tggctattta tttattcttt cttcaaataa ttaaccattg cttttgctgc agtatcagag
      301 gcaattgttg tacctaattc agtatgtaat tgctgatagc ctgccaatat attagcacga
      361 tatgcttggt cgaatactag ctttttcaat tccttttgcg tattttttgg ggtaaaatct
      421 tgtataagta attcttttac taattctttt tgggcaataa tatttaccaa agacacatac
      481 ttaattttca acaccattcg taaaaatgga gctaccaaac gtgataaaaa agcctcataa
      541 cacaccactt gtggcacatt aaaaagagct gtttctaagg ttgctgtgcc cgaggtaaca
      601 atcgctgctt cggcatgttg caataaatca taggtttttc cgtacactat tttagcattg
      661 ctccctttca ttaaggtatg atacagtgta caaggtaacg catcggtacc tgctactata
      721 aactgataat cagcaaattg agaaaaatca acttgcaaca ttatcggcaa acaagcctct
      781 aactcttgct tacgactacc tggtagcaag gcaattattt ttttatcatc taaggcattt
      841 tgttgtataa aaatactgcg attaggtgct gttgcttggt atgtatgcac actttcaacc
      901 gatggattac caacatattg cacctcacat tgatgcttgg cataaaactc agtttcgaaa
      961 ggaaaaatgg taaaaacttt atcaatatac tttttgatag ccttgatacg gtattccttc
     1021 catgcccata tttttggtgg gatatagtaa aaaacaggta attttaaatg gttttttaca
     1081 aatttggcta ttttgagatt aaaaccagga tagtcaatta aaataacttc atcgggttta
     1141 aaatcagtaa tatcttgttg gcaaaaactt aggtttcgga atatagtacg aatatttaat
     1201 aatacaggaa taagcccata aacgccaatt cacgat
//
LOCUS       DAOPUR010000416         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_132590_length_1222_cov_3.982005, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000416 DAOPUR010000000
VERSION     DAOPUR010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132590_length_1222_cov_3.982005"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKN90_06820"
     CDS             <1..730
                     /locus_tag="PKN90_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86024.1"
                     /translation="KAALVPDLFYLYGGIVGDIKHNSYSSILTENNYVSPDVSLQDTY
                     TPLNIYTGLKLKVMDGLLFDGYLGYKIIHDQYFFINRTVNYVAPMPIGTPASAYANTF
                     DVVTEKDASLLSVDLSLTFDKIDGLDVSVNSRYNKWNVSQNAFAWHKPTWEIGLQASY
                     QIDAKWKAGIAYNYMGGRNALVDTEVVAMNNIHDVNATVSYKVFDWLHVFGSLRNVIN
                     ANYDTYYGYKAFGINGMVGASVTF"
     gene            861..>1222
                     /locus_tag="PKN90_06825"
     CDS             861..>1222
                     /locus_tag="PKN90_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HNZ86025.1"
                     /translation="MNFLNFDKNLLINLEKSLTKEMIRTNRAGAYCSSTLVDCNTRKY
                     HGQLVVPLPQIDTYNHVLLSSIDETICKHGAEFNLSVHQYPNNHIFPNGHKYIREFDC
                     NSISKTVFRVGSVIIAKER"
BASE COUNT          405 a          199 c          218 g          400 t
ORIGIN      
        1 taaggctgct ttagttcccg atttatttta tctgtatggt ggcattgtag gggatattaa
       61 acacaattcg tattcatcaa ttttaacaga aaacaactac gtgtcgcccg acgttagttt
      121 gcaagacacc tatacacccc taaatattta cacaggatta aaattaaaag tgatggatgg
      181 gcttttattt gatgggtatt tgggatataa aattatacac gaccagtatt ttttcatcaa
      241 cagaacggtt aattacgtag ctcctatgcc tataggtaca cctgcaagtg catacgcaaa
      301 cacatttgat gtggtaacag aaaaagatgc atcgctttta tcggttgatt tatcgcttac
      361 ttttgacaaa atagatggac tagatgtatc ggttaacagt cgttataaca aatggaatgt
      421 aagtcaaaat gcttttgcgt ggcataaacc aacatgggaa attggattac aagccagcta
      481 tcaaattgat gctaaatgga aagcaggtat tgcctataac tatatgggag gtagaaatgc
      541 cttagttgat acagaagtgg tagccatgaa caatatacat gatgtaaatg ctactgtttc
      601 ttataaagta ttcgattggc tacacgtatt tggatctttg cgaaatgtaa tcaatgcaaa
      661 ttacgatact tattacgggt ataaagcctt tggaatcaat ggaatggtag gtgcttcggt
      721 tacattctaa agtacatagt cctttttttt taagggaaag aagaaaacac ttctttccct
      781 tttttatatc aaaatattat gtatttttgc atttatcata gttagaattg caatttttac
      841 ctatttctta cacaagcagt atgaattttt tgaattttga taaaaatttg ctaattaatt
      901 tagaaaaatc gctcaccaaa gagatgattc gtaccaatcg tgctggagct tattgtagct
      961 ctaccttggt agattgcaat acacgtaaat accacggaca attagtagta ccacttccgc
     1021 aaattgatac ttataatcat gtacttttat cctctattga cgaaacaatt tgcaaacatg
     1081 gtgcagaatt taatttaagc gtacatcaat atccaaacaa ccatattttt ccgaatggac
     1141 ataaatacat tcgtgagttt gactgcaatt ccatctcaaa aacagtattt cgggtaggca
     1201 gtgtaataat tgccaaagaa cg
//
LOCUS       DAOPUR010000417         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_134045_length_1214_cov_3.151855, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000417 DAOPUR010000000
VERSION     DAOPUR010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134045_length_1214_cov_3.151855"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PKN90_06830"
     CDS             <1..831
                     /locus_tag="PKN90_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ86026.1"
                     /translation="QYQQFFPSFFPTIAAAGKVILNQGIDDVAYINEVINKTKNTYTV
                     NNEKVFMLGASMGGAMAYKYAFSANPQITAMAIISGFKGAEVSTSGDFPVATCHFHST
                     NDEVVVYNGTAFNTPIQDLVTLLSGKNHTTTAEVIAIPNAEEEDNMTVTAYDYQTASK
                     PRFLFYKIEGANHMLTGLKDIAIFTEALKFFNNEPILTSITDTYVAQVNVSPNPTSDY
                     IYVSVSGNYTLYTLQGNIILAGSAHENEAIAVGHLPKGLYLLSIENNGTISSAKVAIK
                     "
     gene            836..1018
                     /locus_tag="PKN90_06835"
     CDS             836..1018
                     /locus_tag="PKN90_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HNZ86027.1"
                     /translation="MDIQLIIVIIASILALLWVVWRVYRTFKQSDSAKNACGSCAHSA
                     SCKFKEECNDILHKQS"
     gene            1091..>1214
                     /locus_tag="PKN90_06840"
     CDS             1091..>1214
                     /locus_tag="PKN90_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ86028.1"
                     /translation="MTNAQITNSHTLVKISTEYGDMTVKLYNETPLHRDNFIKLV"
BASE COUNT          408 a          275 c          202 g          329 t
ORIGIN      
        1 caatatcaac aattttttcc tagttttttc cctacaattg cagccgcagg aaaagtaata
       61 ctcaaccaag gcatagacga tgtagcctac atcaacgagg taatcaataa aacaaaaaat
      121 acctacaccg taaacaacga aaaagtattt atgctaggag catctatggg tggtgctatg
      181 gcatacaaat atgcttttag tgctaatccg caaattacgg ctatggctat cattagcggt
      241 tttaagggtg ccgaggtaag cacatcaggg gatttccccg ttgccacctg ccattttcat
      301 agcaccaacg acgaagtagt ggtatacaac ggaactgcat ttaatacacc tatccaagac
      361 ttggttacct tattgtcggg caaaaaccat acaactactg ccgaagtaat tgccataccc
      421 aatgccgaag aagaagataa catgacggta accgcctacg actaccaaac agcaagcaaa
      481 cctcgctttt tgttttacaa aatagaaggt gcaaaccaca tgctaacggg cttaaaagat
      541 attgccattt ttacagaagc tttgaaattt ttcaacaacg aaccaattct tacttctatt
      601 accgacacat acgtagctca agtaaacgtt tctcctaacc ctactagcga ttatatctat
      661 gtatctgtta gtggcaacta tactctgtac acattacaag gcaatatcat actcgctggt
      721 agtgctcacg aaaacgaagc cattgctgtg ggtcatttac ccaaaggctt gtatctacta
      781 tctattgaaa acaacggcac aatcagctct gccaaagtag ccattaaata agtaaatgga
      841 catacaactt atcatagtca ttatagcctc catactcgcc ctattgtggg tagtatggag
      901 ggtgtatcgc acgtttaaac agtccgattc agcaaaaaat gcctgcggta gttgtgccca
      961 tagtgccagt tgcaagttca aagaagaatg caacgatatt ctacacaaac aatcttagtt
     1021 tttcattttt atgaaatcgc tttttttaat caaaaccatt ttaatactcg cacttaccca
     1081 attctctttt atgacaaacg cacaaataac caactcgcac accctcgtaa aaattagtac
     1141 cgaatacggc gatatgaccg taaaattata taacgaaact cctctgcaca gagacaattt
     1201 tatcaaacta gtaa
//
LOCUS       DAOPUR010000418         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_134254_length_1213_cov_2.630397, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000418 DAOPUR010000000
VERSION     DAOPUR010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134254_length_1213_cov_2.630397"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..791
                     /locus_tag="PKN90_06845"
     CDS             90..791
                     /locus_tag="PKN90_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNZ86029.1"
                     /translation="MSIIERLEQERNAAKAQILQGFFKTGKGQYGEGDIFWGISVPTL
                     RGIAKTYYQQIPTEEITQLLHSPIHEQRLLSLYILILRYNHAKDDNEKQAIIDTYLSN
                     TRYINNWDLVDSSAYHLLGKHYVDKPRTILFQLVHSSDLWEQRIAIIATFYFIRQHDY
                     ADTLRMAGILLHHPHDLIHKAVGWMIREVGNRDYETAYAFLVEHYAQMPRTMLRYAIE
                     KFDEPIRLAFLKGEV"
     gene            complement(823..>1213)
                     /locus_tag="PKN90_06850"
     CDS             complement(823..>1213)
                     /locus_tag="PKN90_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86030.1"
                     /translation="FRLLNVKLHKIIQYEWLTKPNNLTEKISKYNEIILQTKCPYFIA
                     IALDFASGFGLDDIKEHFLGRGVEFDCKEDSEHEPSIFYENPQLSGIIVLDNKKFYLL
                     LNPCKKQVIYNKRNEVLLTKICKIPLM"
BASE COUNT          398 a          240 c          205 g          370 t
ORIGIN      
        1 ttatttaaaa attatttcgg cacaaatata cactattcag gcatatttag agaatatcaa
       61 gctatatttg taaaaaaaat tcagcgtata tgagcattat tgaaagactt gaacaagaaa
      121 gaaatgcagc taaagcacaa attttgcaag gattttttaa aactggcaaa ggacaatacg
      181 gcgaaggcga tattttttgg ggcatttcgg tacctaccct acgtggcatt gccaaaacgt
      241 attaccaaca aatacccaca gaagaaatta cccagttgct gcactcgccc attcacgaac
      301 agcgtttact ttcactgtat atcctgatat tgcggtacaa ccatgccaaa gatgataacg
      361 aaaaacaagc catcattgat acctacttga gcaatacccg atacattaat aattgggact
      421 tggtagattc gtcggcttac cacttgttgg gcaaacatta tgttgacaaa ccacgcacta
      481 ttctttttca attagtgcac agtagcgatt tgtgggaaca acgcattgcc atcatcgcca
      541 ccttttattt tatcagacaa cacgattatg ccgacacctt gcgtatggca ggtatcttac
      601 tgcatcatcc ccacgacttg atacacaagg ctgtgggctg gatgatacgc gaagttggca
      661 acagggatta cgaaacagca tacgcttttt tggtagaaca ttacgcacag atgcctcgca
      721 ccatgctacg ctacgccatc gaaaaatttg acgagcctat tcggcttgct tttttgaagg
      781 gggaggtata gattagtaaa aagctctgat taaaataatt atctacataa gtggtatttt
      841 acatatctta gttaacaaaa cctcattcct cttattgtaa attacttgtt tcttgcaagg
      901 atttaaaagc aaataaaatt tcttattatc taaaacgata ataccactta attgggggtt
      961 ttcataaaat attgaaggtt catgttcaga atcttcttta caatcaaatt caactcctct
     1021 tcctaaaaag tgctctttaa tatcatctaa tccaaaacca cttgcaaaat caagagcaat
     1081 tgctataaaa tatggacact tcgtttgcaa aattatctcg ttgtatttcg aaatcttttc
     1141 cgtgagatta ttgggtttag ttagccactc atattggatt atcttatgaa gctttacatt
     1201 cagcaaacga aat
//
LOCUS       DAOPUR010000419         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_135004_length_1209_cov_2.517331, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000419 DAOPUR010000000
VERSION     DAOPUR010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135004_length_1209_cov_2.517331"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1209)
                     /locus_tag="PKN90_06855"
     CDS             complement(<1..>1209)
                     /locus_tag="PKN90_06855"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNZ86031.1"
                     /translation="DYAKSNDFRIMWSHAQDAKANPFSTLSASVNFSTSSYERNNLNS
                     YYNPNLLSQNTRSSTITYTQRFPESPFSLSASVYANQRTRDSIISLSLPNLNVSMSRI
                     YPLKRKQVVGTDRWYEKISMTYTGRFSNSITTKEDKLLTSSFTKDWKNGVEHTLPISA
                     SFTVLNYFTVTPSFSSKLRWYFSQVNQTWQGDALTGAIVTDTLNGFYNVFDYSASVSM
                     QTKLYGFYKPMERIFGSRINMIRHVITPSISVSYKPDYGLPAWGYWGTYQRPLTDGTF
                     SQVFYDKYQSQLFGTPSRGEAGTVGFSIANNLEMKVKNKQDSQKEFTKISIIDNLSVS
                     SSYNLIADSLNWSNPNVSLRLKLHKQYTLNLSLLFDPCTYQLNEYGNPKKVNITQLEK
                     NGVLGRLMST"
BASE COUNT          382 a          229 c          209 g          389 t
ORIGIN      
        1 ccgtactcat taaacgtcct aagaccccat ttttttctaa ttgggtaata tttaccttct
       61 tcggattacc atattcattt aactgatacg tgcacggatc gaataacaaa ctcaggttca
      121 aggtatattg tttgtgcaat ttgagcctta aactcacgtt cggattcgac caattgagcg
      181 aatcagcaat caaattatag ctcgaagata cactcaaatt gtcgataatg ctaattttag
      241 taaattcttt ttgcgaatct tgtttatttt taaccttcat ttccaaattg ttcgcaatgc
      301 taaaacccac tgtaccagcc tctccacgac taggagtgcc aaataattga ctttggtatt
      361 tatcgtaaaa tacctgcgaa aaggttccat cagttaacgg acgttggtat gttccccaat
      421 acccccatgc aggcaagcca taatctggtt tgtatgacac acttattgac ggagttatta
      481 catggcgtat catgttaatc cgactaccaa aaatgcgttc cattggttta taaaaaccat
      541 acaatttggt ttgcatactg acagaggcac tataatcaaa tacattataa aaaccattca
      601 aggtatccgt tacaatagct ccagtcaacg catctccctg ccaagtttga tttacctgac
      661 taaaatacca gcgtaacttt gaactaaacg atggcgtaac agtaaaataa ttaagtactg
      721 tgaaggaggc agaaatgggt aatgtatgtt ctaccccatt tttccaatct ttggtaaagg
      781 aggaagtgag taatttgtct tcttttgtag ttatactatt agaaaatcga cctgtatagg
      841 tcatactaat tttttcgtac cacctatctg tacccaccac ttgttttcgt ttcaatggat
      901 aaatacgact catactcaca tttaagttag gcaacgacaa actgataatt gaatcgcgtg
      961 tacgttggtt agcatataca cttgctgata aagaaaatgg actttctgga aaacgttgcg
     1021 tataggtaat ggtagaacta cgtgtatttt gtgataataa attagggtta taataactat
     1081 tcaaattgtt tctttcgtaa gaagacgtag aaaaattaac actagctgat aaggtagaaa
     1141 atggatttgc tttagcatct tgtgcatgcg accacataat gcggaaatcg ttacttttag
     1201 cataatctg
//
LOCUS       DAOPUR010000420         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_136920_length_1198_cov_3.161855, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000420 DAOPUR010000000
VERSION     DAOPUR010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136920_length_1198_cov_3.161855"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKN90_06860"
     CDS             complement(<1..511)
                     /locus_tag="PKN90_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86032.1"
                     /translation="MPIRTRGYDNSYENTYLNGVNFNALARGIFNYSMLGGLNDAMRN
                     KDVNRGLSPTAYSYGNIAGVTNIDTRASQYALGTRTSVAFSNRSYTLRAQALHARDLG
                     NDLYLAVSGVARWADEGIIEGSFYNSAGLFTALEKKFADKHSLSLTLFAAPTQRGQQA
                     ASLQEVYDLA"
     gene            483..>1198
                     /locus_tag="PKN90_06865"
     CDS             483..>1198
                     /locus_tag="PKN90_06865"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ86033.1"
                     /translation="MSYPRVLIGKEKINYELYSVAFYNVENLFDTIPEPNDHEYTPTG
                     AMQWDSKKYWAKMKNMSYAISKIGSNLSPIGPSILGLAEVENRSVLEDLVKQEAIKHI
                     NYQIIHIDGGDPRGVDCALLYNPRDFYVTNVKSYPYYTSSDSSRRSRDQLVVSGILSG
                     EEIHVIVNHWPSRRGGEVNSRPRRVAAAQLTKHIADSILTIDKNAKIIIMGDLNDDPI
                     NESVKDVLNAKQKKSETPEGG"
BASE COUNT          420 a          235 c          223 g          320 t
ORIGIN      
        1 ccgccaaatc atatacttct tgtaaagatg cggcttgttg tcctcgttgc gtaggtgcgg
       61 caaataatgt aagagaaagg ctgtgtttat cagcaaattt cttttctaaa gcagtaaaaa
      121 gccctgctga attataaaac gaaccttcga ttataccttc atcagcccaa cgtgctacac
      181 ccgatacagc caagtacaaa tcgttgccca aatcacgtgc atggagtgct tgtgcccgca
      241 aggtataaga acgattgcta aaagcaacac tggtgcgtgt acctaatgca tactgactgg
      301 ctctggtatc aatattagtt acaccagcaa tatttccata cgaataagcg gtgggagaca
      361 atcctctatt cacatcctta ttacgcatcg catcatttaa ccctccaagc atcgaatagt
      421 taaatatgcc acgagccaag gcattaaaat taaccccatt caaataggtg ttttcatacg
      481 aattgtcata tcctcgtgtt ctgataggca aagaaaaaat aaattatgaa ttatatagtg
      541 tagcatttta caatgttgaa aacctatttg atacgatacc agaacccaac gaccatgaat
      601 atacaccaac aggtgctatg caatgggata gcaaaaaata ttgggcaaaa atgaagaata
      661 tgtcttatgc tatatcaaaa ataggaagta atctttctcc tataggaccc tccattttag
      721 gtctagctga agtagaaaat cgttcagtat tagaagattt agttaaacaa gaagctatta
      781 aacacataaa ctatcaaatt attcatattg atggaggaga cccacgagga gtagattgtg
      841 cattactata taatccacga gatttttacg taaccaatgt aaaaagttat ccttattaca
      901 caagcagtga ttcatctcgt agaagtcgcg accaattagt agtgtcaggc atattaagtg
      961 gcgaagaaat tcatgtaatc gtaaatcact ggccttcgcg taggggtgga gaagtcaatt
     1021 cacgtccacg aagagtagca gcagctcaac taaccaagca tattgctgat tcaattctta
     1081 ctatcgacaa aaatgctaaa attatcatta tgggtgattt aaatgacgac ccaatcaacg
     1141 aaagtgttaa agatgtactg aatgcaaaac aaaaaaagag cgaaacaccc gaaggcgg
//
LOCUS       DAOPUR010000421         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_140137_length_1181_cov_2.738899, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000421 DAOPUR010000000
VERSION     DAOPUR010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140137_length_1181_cov_2.738899"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKN90_06870"
     CDS             <1..431
                     /locus_tag="PKN90_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ86034.1"
                     /translation="NTEEYTEMLSLVQTAPGPISINSAVFIGYKLHRWQGALSCLLGV
                     ILPSFIIILLIAVAFTEINSNPIVERIFKGIRPAVVALIAVPVIRMIQREKLHWTGGL
                     IALLSIILIWLLGYSPVWVVLGAIIYGIVKEYLTTKTKKP"
     gene            428..949
                     /locus_tag="PKN90_06875"
     CDS             428..949
                     /locus_tag="PKN90_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ86035.1"
                     /translation="MIYLELFWSFFTIGLFGFGGGYSILSLIQHEVVDIQGWLTIADF
                     TNIVAISQVTPGPIAINCATYVGYIATNSILGSAVATIAVSLPSLFIMVLISRFINAF
                     RSNPYVAAALNTLKPAIIGLIASAALLLSNPSNFIDYISFIIFGTVFVAAYCKVNPIF
                     LILLAGMAGLVIY"
     gene            complement(953..>1181)
                     /locus_tag="PKN90_06880"
     CDS             complement(953..>1181)
                     /locus_tag="PKN90_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ86036.1"
                     /translation="LLTVAAKEKISVSENNVRAMQKSFDYQTEKFDSGVSTIYEYNEA
                     KNALTKALSNQTQAMFEFLFRSKAIELYMGK"
BASE COUNT          325 a          238 c          204 g          414 t
ORIGIN      
        1 taaacacaga agaatatacc gaaatgttat ctttggttca aactgcacct ggaccaattt
       61 ctatcaatag tgcggtattt attggctaca aacttcatcg ttggcaagga gccctatctt
      121 gtttgcttgg cgttattctg ccttcgttta ttattatttt actaattgcg gttgccttta
      181 ctgaaataaa ttcaaacccg attgtagaac gcatatttaa gggtattcgc ccagctgtcg
      241 tagccttgat agctgtgcct gtaattagga tgatacaacg cgaaaaactc cactggactg
      301 gtggacttat tgccctcctt agcattatac taatatggtt gttaggttat tctcctgtgt
      361 gggttgtact aggtgcaatt atctatggca ttgtaaaaga ataccttacc acaaaaacga
      421 aaaagccatg atttatttag aattattttg gagctttttc accatcggac tctttggttt
      481 tggtggcggc tattctatat tatcgcttat ccaacacgaa gtagttgata tacaaggatg
      541 gttaacaatt gccgacttta ccaatatagt agctatttca caagtaaccc ctggacccat
      601 tgccatcaat tgtgccacct atgttggtta catagccacc aattcgatac tcggatcggc
      661 tgttgccacc attgctgtat cgctaccctc actatttatt atggttttaa ttagccgatt
      721 tatcaatgct tttagaagca atccgtatgt agcagcagcc ttaaacacat taaaaccagc
      781 cattattgga ttaattgcct cagcagcctt attgcttagt aatccctcca actttattga
      841 ttacatcagt tttatcattt ttggtaccgt attcgtggca gcttattgta aagtcaatcc
      901 aattttttta atccttctgg ctgggatggc tggattggtt atatattaaa atttacttcc
      961 ccatatacaa ctcaatagct ttgcttctga ataaaaactc aaacatggct tgagtttgat
     1021 tggataaagc cttggttaat gcattcttag cctcattata ttcataaata gtggagactc
     1081 cactatcaaa tttttctgtc tgataatcaa atgatttttg cattgcacgg acattatttt
     1141 cagacacact gattttttct tttgccgcca cagttaacaa a
//
LOCUS       DAOPUR010000422         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_141867_length_1172_cov_2.237243, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000422 DAOPUR010000000
VERSION     DAOPUR010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141867_length_1172_cov_2.237243"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..>1172
                     /locus_tag="PKN90_06885"
     CDS             48..>1172
                     /locus_tag="PKN90_06885"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNZ86037.1"
                     /translation="MKRPILITLVVLVLLHVVSAFYFFRIDLTAEQRYTLSPNTKKLL
                     RTLDNTMDVTIYLSGDLNMGFLRLQKATTEMLQEFSRYSDQPIAIQMVNPSKASNEKA
                     RFLQYNALEQQGMVPTIVNEKDAEGKFIQKVVFPWAEMVYQGDTVRVNLLKNIPGKIG
                     DENLHISIESLEYELTDAIRRLANKHTAERVAFIEGHGELSEMQVFDITAALAQYYHV
                     DRGQIGADVRILDEYKVVIVAGATERYTESEKFILDQYIMNGGRVLWLLDGVRTSQEA
                     LTTSATTIGIVNDVNLDDQLFTYGIRIAPILLADKQCAQVRVNVATMGQTPQFELAPW
                     YYFPLLQTSPYHVITKNLSPVRAEFASTVQFVGENNTVTKE"
BASE COUNT          365 a          213 c          251 g          343 t
ORIGIN      
        1 gaatgcaaaa aacgaaattt accttagaga aagcataaaa aatgtatatg aaaagaccta
       61 ttctcataac gcttgttgta ttagtgttgc tacatgtagt atctgcattt tatttttttc
      121 gtattgactt aacagcagag caacgctata ccttgtcgcc aaatactaaa aaattactac
      181 gtaccctaga taatacaatg gatgtaacca tttatttatc aggcgatttg aatatgggtt
      241 ttttgcgatt gcaaaaagca actactgaaa tgttgcaaga gttttctcgt tattccgacc
      301 aacccatagc catacagatg gtgaatccat ccaaagcgag caacgaaaag gctcgttttt
      361 tgcaatacaa tgcgttggag caacaaggta tggtgcctac tattgtaaac gaaaaagatg
      421 ctgaggggaa atttatacaa aaagtagttt ttccatgggc agaaatggtg tatcaaggag
      481 atactgtacg tgttaatttg cttaaaaaca tccctggtaa aataggggat gagaatttgc
      541 atatttcgat tgaaagtctt gaatatgagc tgacagatgc tattcgtagg ttggcaaata
      601 aacatactgc cgaacgtgtg gcgtttatcg agggacatgg cgaattgtcc gaaatgcagg
      661 tgtttgacat cacagccgct ttggcacagt attaccatgt agatagaggg caaattggtg
      721 ctgatgtgcg aattttagat gaatataagg tggtaattgt agctggagca acagaacgtt
      781 acaccgaatc agagaaattt attctcgacc aatatattat gaatggcgga cgcgtacttt
      841 ggttgctcga tggtgtgcga acatcgcaag aagctcttac tacatccgct acaacgatag
      901 ggatcgtcaa tgatgtgaat ttggacgatc agctttttac ctatggtatc cgtattgctc
      961 ctattttact agctgacaaa caatgtgctc aagtgcgtgt taatgttgcc actatggggc
     1021 aaacgcctca gtttgaactg gctccgtggt attatttccc cttgttacaa acatccccat
     1081 atcatgttat taccaaaaac ctctcgcctg tgcgtgccga gtttgcttct accgtacaat
     1141 ttgtaggcga aaataacaca gtaacaaaag aa
//
LOCUS       DAOPUR010000423         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_142849_length_1167_cov_2.220324, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000423 DAOPUR010000000
VERSION     DAOPUR010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142849_length_1167_cov_2.220324"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1167
                     /locus_tag="PKN90_06890"
     CDS             <1..>1167
                     /locus_tag="PKN90_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ86038.1"
                     /translation="MYNTPELLLLGKNVDDKRLIDAKQNVHCHEVVNMQYTSGTTGFP
                     KGVMLSHHNIVNNGLITGENMKFTQDDKLCVCVPLFHCFGVVLAVMNCLTHGSTMVMV
                     EKFDPLVVLASIHKERCTALYGVPTMFISELNHPMFSMFDMSSLRTGIMAGALCPIEL
                     MRQVSDKMFMTIASVYGLTETSPGMTQTRLDDSFEVRCTTVGSDYPHVEVRVINPDTG
                     EECPVGVQGEMCCRGFNIMKGYYKNQAATDEIIDKDGFLHSGDLGVKDENGNYRITGR
                     IKDMIIRGGENIYPREIEEFLYKMPEIKDIQVAAVPSKKYGEEVGAFIILKEGQTLKE
                     EDVREFCRGKISRYKIPKYVFFIDKYPMTGSGKIQKYKLKDMALEMCAQQGIEI"
BASE COUNT          394 a          171 c          270 g          332 t
ORIGIN      
        1 ggatgtacaa taccccagag ttgttgttgc taggtaaaaa tgttgacgat aaacgattaa
       61 tagacgcaaa acagaatgtg cattgtcatg aagtggtaaa tatgcaatac acgtctggta
      121 caactggttt tcctaaagga gtaatgcttt cgcatcataa tattgtaaat aatggcttga
      181 ttacaggtga aaacatgaaa tttacgcaag acgataaatt atgtgtatgt gtaccattgt
      241 ttcattgttt tggagtagta ttggcagtaa tgaattgctt aacgcatggt tctacgatgg
      301 taatggtcga aaaattcgat ccgctagtag tgctcgcgtc tattcataag gaaagatgta
      361 ccgccttgta tggtgtgcct acaatgttta tctctgaact caatcaccca atgttttcaa
      421 tgtttgatat gagttcgtta cgaacaggaa ttatggctgg agcattgtgc cctatcgaac
      481 taatgaggca ggttagcgat aaaatgttta tgacaattgc aagcgtatat ggcttaacgg
      541 aaacatcacc aggtatgaca caaacacgtt tagacgattc gtttgaagtg cgttgtacta
      601 ctgttggaag cgattatcct catgttgaag tacgggttat caatccagat acaggagaag
      661 aatgccctgt aggcgtacaa ggagaaatgt gttgcagagg gtttaatatc atgaaagggt
      721 attataaaaa ccaagctgct actgacgaga taattgataa agatgggttt ttacattcgg
      781 gcgatttggg tgtaaaagat gaaaatggca attaccgtat tacaggtcgt attaaagata
      841 tgattatacg aggaggcgaa aatatctatc ctcgcgagat agaagaattt ttgtataaaa
      901 tgcccgagat aaaagatatt caagttgcgg cggttccatc gaagaaatac ggcgaagaag
      961 taggagcatt tatcattctg aaagaagggc aaaccctgaa agaagaagat gtacgcgaat
     1021 tttgtagagg gaaaatatct cgctataaaa tacccaagta tgtatttttt attgataaat
     1081 acccaatgac aggaagtggt aaaatacaaa aatacaaatt gaaagacatg gctcttgaga
     1141 tgtgtgctca acaagggata gaaattg
//
LOCUS       DAOPUR010000424         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_143187_length_1165_cov_3.452252, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000424 DAOPUR010000000
VERSION     DAOPUR010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143187_length_1165_cov_3.452252"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="PKN90_06895"
     CDS             complement(<1..1152)
                     /locus_tag="PKN90_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86039.1"
                     /translation="MKTDSLKDVSSDIKEFLSRRETLISIFADSLSTHSLVSMSISKN
                     DWKMMKKGFLNNLFPHYKSEEITIQHKDVLLFSYQSDSLYMAFENSIFICTASRELFA
                     KSLIQSDKKTSIFANDKAFQTVKEISSRTIPANLFVNVKQLPFVTKKWFHPDAQRIFQ
                     FTDMYADWCGFDISFMEERIVIAGFSSTSQNNKQIQLFNQQEAGVNTLVQSMPQHTYF
                     FNHSHITQLGVYKKNVQKLLSFPQQQAAKQQHDQLTTSTGENPSLFFDRHFDKEIAFG
                     YAPYTSKFDQNAFVLIKIKDEPEALARLQRMANESSPVATSTKNGNISIIQWQSNGFA
                     GSVFGSQYTFPEEYIAIYKGILYIAPSRNMLVYLLQQKERNRTLHRSSTY"
BASE COUNT          353 a          186 c          225 g          401 t
ORIGIN      
        1 ataggtagac gaacgatgca aggttctgtt acgttctttt tgttgtaata agtacacaag
       61 catattacgc gatggagcaa tgtataaaat ccctttgtaa atggcgatat attcttcggg
      121 aaaagtatac tgagagccaa acacacttcc agcaaatcca ttgctttgcc attgtataat
      181 tgaaatattt ccattttttg tagaggtggc aacgggagaa ctttcattcg ccattcgttg
      241 taatcgggca agtgcttcgg gttcatcttt tattttaatc agcacaaatg cgttttggtc
      301 aaatttgctc gtatagggag catagccgaa agcaatttct ttgtcaaaat gacgatcaaa
      361 aaacaaactc ggattttcac ccgtacttgt ggtgagttga tcatgttgtt gctttgctgc
      421 ttgctgttga ggaaacgaga gtaatttttg tacgtttttt ttgtataccc ctaattgggt
      481 gatatgtgaa tgattaaaaa aataggtgtg ttgaggcatg ctttgtacca aggtattaac
      541 tccagcttct tgttggttaa atagttgtat ctgtttgttg ttttggctag tgctcgagaa
      601 accagcaatt acgatacgtt cctccataaa actaatatcg aatccacacc agtcagcata
      661 catatccgta aactgaaaaa tacgttgagc atcggggtga aaccatttct ttgttacgaa
      721 gggtaattgt tttacattga caaacaaatt agctggaatg gttctactcg aaatttcttt
      781 tacagtctga aaagctttat catttgcaaa aatggatgtt tttttgtcgc tttgaatcag
      841 agatttggca aataattcgc gtgaagctgt acatataaaa atgctatttt caaatgccat
      901 atataggcta tctgattgat atgaaaagag aaggacatct ttgtgttgta tggttatttc
      961 ttctgattta tagtgaggaa aaagattgtt aaggaatcct tttttcatca ttttccaatc
     1021 atttttagaa atactcatac ttactaaaga gtgcgtagat aagctatcag caaaaatact
     1081 aatcagtgtt tcgcgacgac ttaaaaactc ttttatatcc gaagaaacat cttttaatga
     1141 gtctgttttc ataaaaatag catgc
//
LOCUS       DAOPUR010000425         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_143626_length_1163_cov_3.079422, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000425 DAOPUR010000000
VERSION     DAOPUR010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143626_length_1163_cov_3.079422"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="PKN90_06900"
     CDS             <1..1132
                     /locus_tag="PKN90_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86040.1"
                     /translation="KINLSVNDPSGLKRAKVMKLATAMIFTSQGKVILHAGDEFLRTK
                     PLAINDIEKTRAHKTPYTNPEEGIEYFHENTYISNDYTNQLRWDRFTNEFSHIAVPMV
                     EYYQGLIAMRRALPGLRMFDGDNIRKGITFYGMDIKEGAQTSKPKTLGVTDFSDEKLN
                     KLTLKFTNGPANSTYYIAGEVYDKRTDANTTASTMYAVTFNQAGVGEITFSRDQINNF
                     DLGKWGDSKNLNFKLVKTPGTWESIATAYTGSGNNRLRPMDVDDTSTLTVDLSVVDFA
                     TGYVPREVDTFIAYEIDNTLENATAPHISNTNYSRIIVIHNADENVITVDIPTITNPQ
                     EWSVIADGNKAGITPIKKEDTAVKIEMKKVTVPALSMAVIVK"
BASE COUNT          410 a          249 c          220 g          284 t
ORIGIN      
        1 caaaattaac ctttcggtaa acgacccatc gggattaaaa cgtgccaaag taatgaaatt
       61 ggctaccgcc atgattttca cttcgcaagg caaagtaatt ttacatgcag gagatgagtt
      121 tttacgcact aaaccactag caattaatga tattgaaaaa acacgtgctc acaaaactcc
      181 ttacaccaac cccgaagaag gcattgagta tttccacgaa aatacgtata tatctaacga
      241 ctataccaac caacttcgtt gggatcgttt tacaaacgaa tttagccaca ttgctgtgcc
      301 aatggtagaa tattaccaag gcttaattgc tatgcgtcgt gcattgcctg gcttgcgtat
      361 gtttgatggc gataacatca gaaaaggcat tacattctat ggcatggaca taaaagaagg
      421 tgctcaaacc agtaaaccta aaactttggg ggttactgat tttagtgatg aaaaactaaa
      481 caaactaacg ctcaagttta ccaacggtcc tgccaatagc acctactaca tagctggcga
      541 agtgtacgac aaacgcaccg atgccaatac aactgcaagc actatgtacg ccgtaacatt
      601 taaccaagca ggtgtaggcg aaattacctt tagccgcgac caaattaaca attttgactt
      661 aggcaaatgg ggcgactcta aaaacctaaa ctttaaattg gtaaaaactc ctggcacgtg
      721 ggaaagcata gccactgcct atacaggttc gggcaacaat agactaagac ctatggatgt
      781 agatgacact agcacactaa ctgttgattt atcggtagta gattttgcta caggatatgt
      841 gcctcgcgaa gtagatacat ttattgccta cgaaatagac aatacacttg aaaatgctac
      901 tgctccacac atcagcaaca ccaactactc gcgcattatc gtaatacaca atgcagacga
      961 aaatgtcatt actgtggata tcccaacaat taccaaccca caagaatgga gcgtaattgc
     1021 cgatggcaac aaagcaggta tcaccccaat taaaaaggaa gatacagcag taaaaataga
     1081 gatgaagaaa gtaacagtac ctgcactaag catggcggtg attgtaaaat aatactattt
     1141 ttttttgaat tcaacaaagg cag
//
LOCUS       DAOPUR010000426         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_143992_length_1161_cov_3.584087, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000426 DAOPUR010000000
VERSION     DAOPUR010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143992_length_1161_cov_3.584087"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PKN90_06905"
     CDS             complement(<1..1004)
                     /locus_tag="PKN90_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86041.1"
                     /translation="MKTILHKLLIASWLIISPTIAFGRFTTLPTIPEHPSIIGFHIPN
                     NPSAPSGLRNLSLEPKQQLDSIVVAGKEKIVYLYNDNGLHIMLTQYKWNGVSWRPEIQ
                     VDLLYNESNKVATSINYTWNIVLGEWTPQWKTIYTYNVYGQTLTEQYITYDRNTKKWE
                     NIKRYAYTYTNAGVITKSTHANWNTITNTWIVDWTTDYVYDIKKQRIADVSYSEGYVP
                     ASKTEYTYNANGALVRKNDFYSNNTTNRWEPSAKEEYLLDNQLRPVVKTYSFWNSILQ
                     GWENLYKSNVEFDNNGNSLSIINYKWNVEHLRWDITDKQEAQVNASGDITALHYFDWN
                     T"
BASE COUNT          330 a          191 c          187 g          453 t
ORIGIN      
        1 gtattccaat caaaataatg taatgccgta atatccccac tggcatttac ctgagcttct
       61 tgtttatcag taatatccca acgcaaatgc tcaacattcc atttataatt aatgattgat
      121 aagctatttc cattgttatc aaattcaaca ttgcttttat ataggttttc ccatccttgc
      181 aatatgctgt tccaaaaact atatgttttt acaactggtc gtagttgatt atccaatagg
      241 tattcctctt ttgccgatgg ttcccatcta ttggtggtgt tatttgaata aaaatcattt
      301 ttgcgtacta aagctccatt tgcattgtat gtgtactctg ttttagaagc gggtacatat
      361 ccttcgctgt acgacacatc tgcaatacgt tgttttttta tatcgtacac gtaatccgtt
      421 gtccagtcta ctatccaggt atttgttatt gtgttccaat tagcatgagt gctttttgtt
      481 ataacacctg cattggtata ggtataagca tatcttttaa tgttttccca tttttttgtg
      541 ttacggtcat atgtgatgta ttgttctgta agtgtttgtc catatacgtt atatgtatat
      601 attgttttcc attgaggagt ccattctccc aacacaatat tccacgtata attgatagat
      661 gtagctactt tattcgattc attatacagc aaatctactt gtatttctgg tcgccaagac
      721 actccattcc atttatattg tgtaagcatg atatgtaatc cattgtcatt atataggtat
      781 acaatttttt cttttcccgc tacaacaata ctgtctagtt gttgtttggg ttcaagagaa
      841 agattgcgta atcccgatgg tgcagaaggg ttgtttggaa tgtgaaaccc aattatactt
      901 ggatgttcag gtatagtagg taaggtagta aaacgaccaa acgcaattgt aggcgaaata
      961 ataagccaac ttgcaattag taatttatgt agtattgttt tcatgatatt catttttagt
     1021 ttttttgctt aatctaacaa attataattt gtatagtatt atcggcgaaa ttctgataca
     1081 aatgtaatag gtatgctatc aaatcagaat tatttacacg tatttttaca tgttatttaa
     1141 cataaataca gtaagatttt a
//
LOCUS       DAOPUR010000427         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_144008_length_1161_cov_3.291139, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000427 DAOPUR010000000
VERSION     DAOPUR010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144008_length_1161_cov_3.291139"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKN90_06910"
     CDS             <1..464
                     /locus_tag="PKN90_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ86042.1"
                     /translation="SSISCSHLYIMYITSSTEEDFILAMYKHGVSEVVNLHTPEAILQ
                     ARANKLAEHIIRCTNAHHKEELSLDPLLLSVSKGDITLQFTKKEFDILHLLSNHPNKT
                     FSRSEIYQKVWGNGIVVSDRTLDVHISKIRTKIGEQFIQTYKGVGYRFMPR"
     gene            470..>1161
                     /gene="truA"
                     /locus_tag="PKN90_06915"
     CDS             470..>1161
                     /gene="truA"
                     /locus_tag="PKN90_06915"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNZ86043.1"
                     /translation="MNEQPYFRYFVYLAYNGSSYHGWQIQPNGISVQEVLEKSFSTLL
                     KQPIAITGAGRTDTGVHANMMVAHFDTHTTIDDCTRLVGKLNAFLPQSIAIFAIKQVQ
                     PTAHARFDALSRTYQYHITTQKNPFTNQVSCKVPPTIDVNKMNTAAEKLLQYADFTSF
                     SKLHTDVKTNICTIHQAQWTQVSDTEWVFTIQADRFLRNMVRAIVGTLFDVGNGKITI
                     EEFCAIIEEKNR"
BASE COUNT          386 a          261 c          207 g          307 t
ORIGIN      
        1 aatcttcgat atcctgtagt catctgtaca ttatgtatat tacctcctct acagaggaag
       61 actttatcct tgcaatgtac aaacacggtg taagcgaagt ggtaaatctg cataccccag
      121 aagctattct gcaagcaaga gctaacaagc ttgccgaaca tattatacgg tgtaccaatg
      181 cccaccacaa agaagaatta tcgctcgacc ctttattatt atccgtaagc aaaggtgata
      241 ttacactgca atttacaaaa aaagaatttg atatcttaca cttattatcc aatcatccca
      301 acaaaacctt ttctcgctcc gagatttacc aaaaagtatg gggtaatggc attgttgtaa
      361 gcgacagaac cttagatgta cacattagta agattcgcac aaaaattggg gaacagttta
      421 ttcaaacata caagggagta ggataccgtt ttatgccacg ttgagctcta tgaacgaaca
      481 gccttatttt cgctattttg tgtatctagc ctacaatggt agctcgtatc acggttggca
      541 aatacaaccc aatggaataa gtgtgcaaga agtgctcgaa aaatcttttt ctaccctact
      601 aaaacaaccc attgccatta ctggagctgg acgcacagat acgggtgtac acgccaacat
      661 gatggttgcc catttcgaca cgcacacgac tatcgacgat tgcacaaggc tggtaggcaa
      721 gttaaacgca tttttaccac aatccattgc catttttgct atcaaacaag tacaacccac
      781 tgctcacgct cggttcgatg cactctcacg tacctatcaa taccacatta ctacacaaaa
      841 aaatcctttt accaaccaag taagttgcaa agtaccacca accattgatg taaataaaat
      901 gaacactgca gcagaaaaac tactgcaata tgctgatttt acgagtttta gcaaattaca
      961 tactgacgta aaaaccaata tttgcaccat acaccaagcc caatggacac aagtaagcga
     1021 tactgaatgg gtatttacca tacaagccga ccgattttta cgcaacatgg tgcgtgctat
     1081 tgtgggtact ctgtttgatg tgggaaacgg aaaaataacc atcgaggagt tttgtgctat
     1141 tatcgaagag aaaaaccgct g
//
LOCUS       DAOPUR010000428         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_150839_length_1128_cov_4.192917, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000428 DAOPUR010000000
VERSION     DAOPUR010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150839_length_1128_cov_4.192917"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PKN90_06920"
     CDS             <1..629
                     /locus_tag="PKN90_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNZ86044.1"
                     /translation="SAQKASHKTPPITSKDIVLFGSFFALNSTVRQQVTTLLDTANKE
                     NAMVYYDVNFRKTHISKRRELLPALIENMKYADIVKGSEEDFDHLYDEKDPITIYQKY
                     IAPHCSTLIYTQGANGAMVFTPTYSLHVPSIPIVPISTIGAGDTFNAGIIFALLQQNI
                     AKDTLCNVQQHEWEAILQQAIDCATHVCMLYDNYITVDFANKKLDLFR"
     gene            complement(618..935)
                     /locus_tag="PKN90_06925"
     CDS             complement(618..935)
                     /locus_tag="PKN90_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNZ86045.1"
                     /translation="MDLHTIDGVRLHAWNSFPSGHTMTAFAFFLSLALIVKNQLLKFF
                     FFAMSLLVGFSRIYLNQHFLEDTLAGSLLAVVIALTMYPFFNTQQTWGNKGLMSLANK
                     STK"
BASE COUNT          398 a          220 c          200 g          310 t
ORIGIN      
        1 attctgctca aaaagcttca cataaaacac cgcccattac aagcaaggat attgtattgt
       61 ttggctcatt ctttgcctta aatagtactg tcaggcaaca agttacaaca ttacttgata
      121 ccgctaataa agagaatgcg atggtgtatt acgatgtaaa ttttagaaaa acacacatca
      181 gcaaacgccg cgaattactc cctgctttga ttgaaaatat gaaatatgcg gatattgtaa
      241 aaggttctga ggaggatttt gatcatcttt acgatgaaaa agacccaata acgatttacc
      301 aaaaatacat tgctccccat tgttcaacgc ttatttatac acaaggagct aatggtgcaa
      361 tggtatttac acccacctat tcgctacacg taccttctat acccattgtt ccaataagta
      421 ctatcggtgc tggagatact tttaatgctg gaataatatt tgccttgttg caacagaaca
      481 ttgcaaaaga tactttatgc aatgtccaac agcacgaatg ggaagccatc ctacaacaag
      541 cgattgattg tgctacacat gtgtgtatgc tctacgataa ttacatcacc gtagattttg
      601 ccaataaaaa actggattta tttcgttgat ttatttgcta aagacattag ccctttgttt
      661 ccccatgttt gttgggtatt aaaaaatggg tacatcgtca atgcaatcac aacggcaagt
      721 aaggatcctg ctaaggtatc ttctaaaaaa tgctggttca aataaatacg tgaaaaaccg
      781 accaataacg acatggcaaa aaagaagaat ttgagtagct gattttttac aatcaaggca
      841 agtgataaga aaaaagcaaa tgctgtcatt gtatgaccag acgggaaact gttccacgca
      901 tgcaaacgaa cgccatcaat cgtatgtaaa tccaagccta actcttgaaa aactatgcga
      961 ggaggggagc cctaaacaga ttttttagag tatataccac aattgcacca accacctgaa
     1021 ctgacaatac aaaaagagct ttgcgtagat gaaataggag taaaattcct ccaacaataa
     1081 acggaaaggt ttctcctaat ttagtaatat acggaaacaa ggaatcac
//
LOCUS       DAOPUR010000429         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_151506_length_1125_cov_3.130841, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000429 DAOPUR010000000
VERSION     DAOPUR010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151506_length_1125_cov_3.130841"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1125
                     /locus_tag="PKN90_06930"
     CDS             <1..>1125
                     /locus_tag="PKN90_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNZ86046.1"
                     /translation="EWKLSYDGRNREPVTLPVKFPLLLAQGIEGIAVGLSSKILPHNF
                     NELIDASIAHLKGEDFEIFPDFIGGGYIDVSRYNQGERGGSVKVRAKISKLDSKTLVI
                     NELPFGETTSSLIDSIVKASEKGKIHIKRVDDNTSANVEILVHLAPGKSSDKTIDALY
                     AFTNCEVSISPNCCVIYEDKPHFLTVNTVLKHSTDHTLNLLKEELLLQKAELQESIFF
                     SSLEKIFIEQRIYKDKEFEESKNLAEAVAHIDKRLVPFKKLFIREVETEDIERLMEIK
                     MGRILKFNSDKADQHIANLNEQLEQVKHHLAHIVEYTISWFVGLKEKYGNLYPRKTEI
                     RNFDTIEVTKVVEANQKLYFNREEGFIGTGLKKDEFVCNCS"
BASE COUNT          386 a          166 c          237 g          336 t
ORIGIN      
        1 cgagtggaaa ttgtcgtatg atggaagaaa cagagagcct gtaactttac ccgttaaatt
       61 tcctttgtta ttagctcaag gaatagaggg tattgcagta ggattgtctt caaaaatatt
      121 gccacacaat tttaatgagt taattgacgc ttctattgcc catctcaaag gcgaggattt
      181 tgaaatcttt cctgatttta tcgggggagg atatattgat gtgagtcggt acaaccaagg
      241 cgagcgtggc ggaagtgtaa aagtgcgtgc taaaattagc aaattagata gtaaaacgct
      301 ggtaataaat gagctaccat ttggcgaaac gactagttcg ttgattgact cgattgtaaa
      361 agcatcggaa aaaggcaaaa ttcatatcaa aagggtggac gataatactt ctgctaatgt
      421 ggaaatttta gtgcatttgg ctccaggaaa atcatcggac aaaaccattg atgccttgta
      481 cgcattcacg aattgtgaag tgagtatatc gcccaattgt tgtgttatat acgaagataa
      541 acctcatttt ttaaccgtta atactgtact aaagcattct acagatcata ctctcaactt
      601 attgaaagag gagttgttgc tacaaaaagc tgaattgcaa gaaagtatct ttttctcttc
      661 gctcgaaaaa atatttatcg aacaacgtat ttataaagat aaagagtttg aagaaagtaa
      721 aaacctggct gaggcagtag ctcatattga taaacgatta gtccctttta aaaagctatt
      781 tatccgcgaa gtagaaacag aagatattga gcgtttgatg gaaataaaaa tggggcgtat
      841 cctcaaattt aattctgata aggctgacca gcatattgct aatttaaatg agcaattgga
      901 acaagtaaaa caccatttgg ctcatattgt ggaatacaca atcagttggt ttgttggatt
      961 aaaagagaaa tatggtaatc tatatcctcg taaaactgaa attcgtaatt tcgataccat
     1021 agaggtaact aaggtagttg aagccaatca gaaattgtat tttaatcgcg aagaaggttt
     1081 tatcggaaca ggcttaaaaa aggatgagtt tgtgtgtaat tgttc
//
LOCUS       DAOPUR010000430         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_151555_length_1125_cov_2.361682, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000430 DAOPUR010000000
VERSION     DAOPUR010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151555_length_1125_cov_2.361682"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKN90_06935"
     CDS             <1..371
                     /locus_tag="PKN90_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNZ86047.1"
                     /translation="KMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEAAKQAVEN
                     DVHIMGVSSLAAGHKTLVPQVIAELKALGREDIIVIAGGVIPAQDYDYLYQAGVAAIF
                     GPGTSVAKAAVTIMEILLED"
     gene            complement(440..727)
                     /locus_tag="PKN90_06940"
     CDS             complement(440..727)
                     /locus_tag="PKN90_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNZ86048.1"
                     /translation="MHKTILSGIVHEIPEDIAKILLENDAILDKWNALTPIQRNEWIC
                     WVTIVKKEQTRAEHIIRMMDELKAGKRKPCCWPGCPHRRSSAQKWFTNKSM"
     gene            complement(720..>1125)
                     /locus_tag="PKN90_06945"
     CDS             complement(720..>1125)
                     /locus_tag="PKN90_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ86049.1"
                     /translation="TKNRPAKPEMLLEHYLLRNEKQNKSYAYDTPKGDAKKATLTIKE
                     LATSTNYTLLEVDLQTGRHHQIRCQLAHIGCPIKGDLKYGFARSNPDGGISLHARQIN
                     FIHPVTKEPIEIIAPTPEDNLWKAFEDMIRNA"
BASE COUNT          292 a          201 c          237 g          395 t
ORIGIN      
        1 ccaaaatggg tcaagacgga cacgaccgcg gtgcaaaagt tgtagccaca gggtatgccg
       61 attgtggttt tgatgtggat atgggacctt tgttccaaac gccagccgaa gcagccaaac
      121 aggctgttga aaatgacgta cacataatgg gcgtttcatc attagctgca ggtcacaaaa
      181 ccttagtccc acaagttatt gctgaactaa aagccttggg acgtgaagat atcattgtaa
      241 tagcgggtgg ggtaattcct gcacaagatt atgattatct atatcaagca ggtgttgctg
      301 ccatctttgg tcctggaaca tcggttgcaa aagctgctgt tactattatg gagattttgt
      361 tagaagacta agatagtttt ttactttata aaaaaacgag gttgtaatta tgtgttacaa
      421 cctcgttttt gttttctgat tacatagatt tgttagtaaa ccatttttga gcagatgaac
      481 gtctgtgtgg acatcctggc caacaacacg gtttacgttt ccctgccttt aattcatcca
      541 tcatacgtat aatatgttct gctcttgttt gctctttttt tacaatggta acccaacaaa
      601 tccattcatt tcgttgtata ggtgtaagtg cattccactt gtcaagaata gcatcatttt
      661 caagcaatat ttttgctata tcttcaggaa tttcatgtac tatgcccgaa agaattgttt
      721 tatgcatttc ttatcatgtc ttcaaaagcc ttccaaagat tatcttctgg tgttggggca
      781 ataatctcaa ttggttcttt ggttactgga tgtataaaat ttatttgtcg ggcgtgtagg
      841 ctaatacctc catcaggatt tgagcgtgca aagccatatt ttaagtctcc ttttattggg
      901 caaccgatat gtgctaattg acaacgaatt tggtgatgtc tgcctgtttg taaatctact
      961 tcaagtaatg tataattggt gctggtagcc aattctttaa ttgtaagagt tgcttttttt
     1021 gcatcgcctt taggcgtatc gtaggcatac gatttatttt gcttttcgtt gcgaagcagg
     1081 tagtgctcta atagcatttc tggtttagct ggtcggtttt ttgta
//
LOCUS       DAOPUR010000431         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_154161_length_1113_cov_2.813800, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000431 DAOPUR010000000
VERSION     DAOPUR010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154161_length_1113_cov_2.813800"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PKN90_06950"
     CDS             complement(<1..360)
                     /locus_tag="PKN90_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86050.1"
                     /translation="MKKITTLCIALLVASITALAATKTTETFELAGKTRTYLQYIPSS
                     YNAETPSKVLILLHGLNGNMNFFENQGDNLATMADVTNTIILSLQALDEQDTEVNTLI
                     GLLGTSQATSFTNTGVWG"
     gene            complement(495..>1113)
                     /locus_tag="PKN90_06955"
     CDS             complement(495..>1113)
                     /locus_tag="PKN90_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNZ86051.1"
                     /translation="WEKSQQYLRKMGTIILFASIIIWALGYFPLAETRTPQLDDKINQ
                     LTALPTTEETNTQLQKLQWERNTQQQKTSYIGKIGNTILPIIKPLGFNWEMGIGLIAG
                     LPAKELVVSTLGVLYTNNATDENSAELSYKLQHVTDASGKQQITPLIAFGYMIFILLS
                     FPCIATIIAIKNESGSWKWGLFSIVYSTSLAWVCAFLIFQLGSLL"
BASE COUNT          305 a          199 c          238 g          371 t
ORIGIN      
        1 accccatacg cccgtattgg taaacgaggt agcttgagaa gttccgagaa gtcctattaa
       61 agtatttact tcggtatctt gctcatctaa ggcttgcaac gataaaataa tggtgttggt
      121 aacatctgcc atagtcgcta agttgtcgcc ttggttttcg aaaaaattca tgttgccatt
      181 cagtccgtgt aacaggatca ataccttgct aggtgtttcg gcattgtagg agctagggat
      241 gtattgcagg taggtgcgtg ttttgcctgc taactcaaag gtttcggtgg ttttagtagc
      301 agccaatgct gtaattgatg ctaccaatag ggcaatgcaa agtgtagtga tttttttcat
      361 acagtttttt tgtttttttt gtcttacacg cttattgtat ttgcaaacgc ataagtagtt
      421 tatactaagt taatttacta tgcaaaggta ataaaatttt agaaattact acttttttta
      481 agattatttt ttttctataa caaactccct aattgaaaaa tgagaaatgc acatacccat
      541 gccaaagatg tgctgtacac aatagagaat aatccccatt tccacgagcc cgattcgttc
      601 ttaatagcaa tgatagtagc gatgcacgga aaactcaaca gaataaaaat catgtagcca
      661 aaagcgataa gcggtgtaat ttgttgcttg cccgaggcat cggttacatg ttgtagttta
      721 tacgatagtt cggctgaatt ttcgtcggtt gcattgttgg tgtataatac gcccaatgtg
      781 ctaacaacta gctctttggc aggtagtcca gcaataagac caattcccat ttcccaatta
      841 aaacccaggg gtttgataat agggagaatc gtattgccaa ttttaccaat ataactcgtt
      901 ttttgttgtt gcgtgttacg ttcccattgc agtttttgca gttgcgtgtt cgtttcctcg
      961 gtggtgggaa gtgctgttag ttggttgatt ttatcatcta gttgcggtgt acgcgtttct
     1021 gctaagggga aatagcctaa agcccagata atgatagagg caaacaagat gatggtaccc
     1081 attttacgca agtattgttg cgatttttcc cac
//
LOCUS       DAOPUR010000432         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_154807_length_1110_cov_3.209479, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000432 DAOPUR010000000
VERSION     DAOPUR010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154807_length_1110_cov_3.209479"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PKN90_06960"
     CDS             complement(<1..1106)
                     /locus_tag="PKN90_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNZ86052.1"
                     /translation="MLIPPTALQGTVVLPTSKSISNRVLIINALAESNMPIEGVSDCD
                     DTRVMLNVLQSNDTKFDVGAAGTSMRFLTAFLSKIVGKWEITGSERMKNRPIKLLVDA
                     LNELGAKIEYIEKEGYPPLRIYGSALQGGRIEMQGNVSSQYISALLMLAPTMQQGLRL
                     VLQGDIISVPYIQMTIKIMEQFGIQSYWKDNEIYIPNQAYTPVPFVVEGDWSAASYWY
                     EMLALAPQGEVELKGLYKDSVQGDAAIATIFEHIGVKTKFTKQGVILSKKAPKKGKLV
                     YNFVNQPDLAQTCVVCCCLLGIPFYFSGLQSLKIKETDRIYALVTELKKLGYVVHQKD
                     NSILEFDNERCDVATEQSIDTYDDHRMAMAFAPA"
BASE COUNT          327 a          253 c          167 g          363 t
ORIGIN      
        1 caagcaggag caaatgccat agccatacga tgatcatcat atgtatctat gctctgttct
       61 gtagccacat cacaccgttc attatcaaac tctaaaatac tattgtcttt ttgatgaacg
      121 acatatccta gcttttttaa ttccgtaacc aaggcataga ttctgtctgt ttctttaatt
      181 tttaaacttt gtaaacctga aaaataaaaa ggtatcccta ataaacagca acacacaaca
      241 caggtttgag ccaaatcagg ctgatttaca aaattatata ctagtttgcc cttttttgga
      301 gctttcttag ataagattac tccttgcttt gtaaactttg ttttcacacc aatgtgttca
      361 aaaattgtag caatagcagc atcaccctgc acactatctt tatacaagcc tttcaattct
      421 acctctcctt gtggtgctaa tgcaagcatt tcgtaccaat acgaagctgc cgaccaatct
      481 ccttctacca cgaagggaac tggtgtataa gcttggttag gaatgtatat ttcattgtct
      541 ttccagtatg attgaatacc aaactgctcc attattttta tcgtcatttg tatgtatggc
      601 accgaaataa tatcgccttg caacactaac cgcaatccct gttgcatagt aggtgctaac
      661 attaacaaag cagaaatata ctgactacta acattccctt gcatttcaat acgaccaccc
      721 tgcaatgcac tgccataaat acgcaacggt ggatatccct ctttctcaat gtattcaatt
      781 tttgctccta actcattcag tgcatcaacc agtagtttaa tgggtctgtt tttcatgcgt
      841 tcgcttccag ttatttccca tttacctact atcttagata gaaatgctgt taaaaaacgc
      901 atagacgtgc ctgctgctcc tacgtcaaac tttgtatcat ttgattgcaa tacgtttaac
      961 atcacacgcg tgtcgtcgca atcagaaacg ccctcaatag gcatattgct ttctgccaaa
     1021 gcattaataa tcaacactct gttacttata cttttagatg ttggcaatac tactgtacct
     1081 tgtaacgcag tgggcggtat taacaaatat
//
LOCUS       DAOPUR010000433         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_162299_length_1078_cov_2.493646, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000433 DAOPUR010000000
VERSION     DAOPUR010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162299_length_1078_cov_2.493646"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>234
                     /locus_tag="PKN90_06965"
     CDS             <1..>234
                     /locus_tag="PKN90_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNZ86053.1"
                     /translation="AYLYWKETGDISVWKGIAQDALIMNIDDLICVGAIDNILVSSTI
                     GRNKLLIPGEVIAAIINGTEELLAELRELGIGVY"
     assembly_gap    235..244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <245..958
                     /locus_tag="PKN90_06970"
     CDS             <245..958
                     /locus_tag="PKN90_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNZ86054.1"
                     /translation="DLVRTIIVDSTVTCRMKRADVIDNARIEGGDVIVGLASYGQATY
                     EKSYNGGMGSNGLTSARHDVFANYLANKYPESYDASMPSELAYSGSLQLTDAVPNSPI
                     DAGKLVLSPTRTYAPVVKQLLDTMRNSIHGMVHCSGGAQTKILHFIDNLHVIKDNLFP
                     VPPLFKTIQEESGTDWAEMYKVFNMGHRLEVYLPKEYAQQVIDIAQSFGIDAQVIGRV
                     EASDNKKLTIKSEFGTFVY"
BASE COUNT          352 a          196 c          215 g          305 t
ORIGIN      
        1 gcctatttgt attggaaaga aacaggcgat atatctgtat ggaaaggcat agcacaagat
       61 gctttgataa tgaatattga cgatttaatt tgtgtgggag ctattgataa tatattggtt
      121 tcttcaacaa ttggacgcaa taaactcctt attccaggcg aagttatcgc agccattatc
      181 aatggcacag aagagttgct agccgaacta cgtgaactag gtattggtgt atatnnnnnn
      241 nnnngattta gtaagaacca tcatcgtaga tagtacagtt acttgccgca tgaaacgtgc
      301 tgatgtaatt gacaatgcac gcattgaggg aggcgatgta attgttggac ttgcctctta
      361 cggtcaagca acgtacgaaa aatcctataa cggtggtatg ggtagcaatg gactaacttc
      421 ggctcgccat gatgtatttg ctaactattt agccaataag tatcctgaaa gttacgatgc
      481 aagtatgccc tctgaattgg catattcggg gtcgttacaa ctcacagatg ccgttcccaa
      541 ttcgccaata gatgctggta aattagtact ctctcctact cgcacatatg ctccagtagt
      601 aaagcaatta ttagacacaa tgcgtaattc tattcatggc atggtgcatt gctcaggagg
      661 tgctcaaaca aaaatattgc actttataga caatctacac gttattaaag ataacttatt
      721 tccagtacct cccttattta aaaccattca agaagaaagt ggtacggatt gggcagaaat
      781 gtacaaagta tttaatatgg gacaccgact agaagtatat ttgccaaaag aatacgctca
      841 gcaagtaatc gacatcgcac aatcgtttgg catagatgca caagttatag gaagagtaga
      901 agcctccgat aacaaaaaat taaccatcaa gagtgaattc ggcacttttg tatattaaca
      961 agcccgaaaa acatatatga gcacattatt gcaaaaatta gaaggtttag ctactcgttt
     1021 tgaagaagtt tctacattga ttacaccagc ttcaaacatt ggatgtttca aaacctta
//
LOCUS       DAOPUR010000434         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_163535_length_1073_cov_2.375246, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000434 DAOPUR010000000
VERSION     DAOPUR010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163535_length_1073_cov_2.375246"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKN90_06975"
     CDS             complement(<1..204)
                     /locus_tag="PKN90_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNZ86055.1"
                     /translation="MNAIKIVLNGILKENPVFVLLLGMCPTLGTTSSAINGLGMGIAT
                     MFVLIGSNTVISLIKNVIPDKIRI"
     gene            complement(219..842)
                     /locus_tag="PKN90_06980"
     CDS             complement(219..842)
                     /locus_tag="PKN90_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNZ86056.1"
                     /translation="MKKLESNLPNMVMVLTAITLIASFLLASVYGVTKAPIELAKEKK
                     KEVAIKNVLPPFNRIENDTIEGIVVYKGYKDANWVGTAVESVSKNGFGGEIKIIVGFD
                     AKGCIFNYTVLEQKETPGLGTKMIDWFKTSKNKQSILGKNIAQEELKVSKDGGNIDAM
                     TASTISSRAFLEAVNNAYSTISHYDTHTSATQVSDAATAATPWVENK"
     gene            complement(869..>1073)
                     /locus_tag="PKN90_06985"
     CDS             complement(869..>1073)
                     /locus_tag="PKN90_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNZ86057.1"
                     /translation="DYATSPMNTVAQLVYGIGIGLITVTIRLFGAYPEGVSFAILIMN
                     GVTPLLNLYVKPKRFGAPKTKKN"
BASE COUNT          318 a          215 c          150 g          390 t
ORIGIN      
        1 aatgcgaatt ttatcaggta ttacattctt aatcagagaa attaccgtat ttgaaccaat
       61 aagaacaaac atagtagcta tgcccattcc gagcccatta atagcagaac ttgtagttcc
      121 taaagtgggg cacatcccca gtaacaaaac aaaaacagga ttttctttta atatcccatt
      181 tagaacgatt ttaattgcat tcatgtatcc tattgttttt atttattttc tacccatggt
      241 gtagctgctg tagcagcatc tgatacttgt gtagcactag tatgtgtgtc gtaatgtgat
      301 atggtggaat aggcattatt caccgcttct aaaaacgccc ttgaactaat cgttgaggct
      361 gtcattgcat caatatttcc accatctttt gaaaccttta actcttcttg agctatattc
      421 ttaccaagaa tactttgctt attctttgaa gttttaaacc aatctatcat tttagtacct
      481 agtcctggcg tttccttttg ttctaaaact gtataattga aaatacatcc ctttgcatca
      541 aaacctacaa ttatttttat ttctccacca aacccatttt ttgatacact ttctacagct
      601 gttcctaccc agttagcatc tttataacct ttgtatacaa caattccctc aattgtgtca
      661 ttttctatcc gattaaaagg aggcagtaca tttttaattg cgacttcttt ctttttttct
      721 ttagccaact caattggtgc tttagtaaca ccataaacac ttgccaacaa aaatgatgct
      781 attaaggtaa tagcagtcaa aaccatgacc atattaggca aatttgattc taattttttc
      841 atttagtatg ttattttgaa tacctacttt agtttttttt cgtttttggt gccccaaatc
      901 gtttgggttt aacatataga ttcaataatg gtgtaacacc attcataatc aatatagcaa
      961 aagatactcc ctctggatag gctccaaata aacgtattgt tactgtaatc agtccgatac
     1021 ctattccgta taccaactgt gctactgtat tcataggcga tgtggcataa tca
//
LOCUS       DAOPUR010000435         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_172198_length_1039_cov_2.689024, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000435 DAOPUR010000000
VERSION     DAOPUR010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172198_length_1039_cov_2.689024"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..795
                     /locus_tag="PKN90_06990"
     CDS             157..795
                     /locus_tag="PKN90_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86058.1"
                     /translation="MLYKVPDTYKLHPKSIVLKSLKEWAYLAPSVFTPNISVLADYAI
                     TPHRYLFVREVSVGTSNYASQTADSILQIAKLIPSDMPVLLSLEKKDKYKEYPQNWIL
                     LQEPLQDVHSLIYYSAGLISSGDSMAREASLLGVPSYYLGIRYSMPANAVAAEVGSLD
                     NQQTMSIEKWIEKTLSLQENKPLLQEEVRASINEKFVDIHAYMYNLVTQHQQ"
     gene            796..>1039
                     /locus_tag="PKN90_06995"
     CDS             796..>1039
                     /locus_tag="PKN90_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86059.1"
                     /translation="MRDFTLQAYKDLLIALQQSAYQFLTFEQYCIAKQSNGALPPKVV
                     ILRHDVDLKAANSLATAQLEAELGIVASYYFRVVPQS"
BASE COUNT          345 a          191 c          189 g          314 t
ORIGIN      
        1 tgggcatata aaaaaaatct agctatgtca atttcaggct ctgtaccatg ctccctcgtg
       61 tcaagagtat ttaattgtaa accgtatact ttaattgatg atccccaggc tgtaacgtat
      121 tcaacaattg caaatttaag ctataagtct ttttgtatgt tgtacaaagt accagacaca
      181 tacaagctac accctaaatc catcgtgctt aaatcattaa aagaatgggc atatttagca
      241 cctagcgttt ttactccaaa tatatccgtg ttagcagact atgctattac gccacatcgt
      301 tacctttttg taagagaagt gagtgtaggt acttcaaatt atgcatcgca aactgctgat
      361 agtatattac aaattgccaa gcttattcct agtgatatgc ctgtattgct ttcgttagaa
      421 aagaaagata agtacaaaga atacccacaa aattggattt tgttgcaaga accacttcaa
      481 gatgtacatt cactgattta ttacagtgca ggtcttattt catcggggga tagtatggct
      541 cgcgaagctt ctttattggg tgtgccttcg tactatttgg gcattaggta ttcaatgcct
      601 gccaatgctg ttgctgcaga agtaggtagc ttagacaacc aacaaacaat gagtatagaa
      661 aagtggattg aaaaaacctt atcgttacaa gaaaataaac cattgttaca agaagaagta
      721 cgtgcttcaa taaacgaaaa attcgtagat atacatgctt atatgtacaa tcttgtaaca
      781 caacatcaac aataaatgag agattttacc ctacaagcat acaaagattt actgatagca
      841 ttacaacagt ctgcttatca atttcttaca ttcgaacaat attgtattgc caaacaaagt
      901 aacggggcat tgccacccaa ggtggttata ttgcgtcatg atgtcgattt aaaagcagca
      961 aactcattgg ctacagcaca gcttgaggct gagttgggta tagttgctag ctattatttt
     1021 agggtagtac cacaaagca
//
LOCUS       DAOPUR010000436         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_179470_length_1013_cov_1.889353, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000436 DAOPUR010000000
VERSION     DAOPUR010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179470_length_1013_cov_1.889353"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKN90_07000"
     CDS             complement(<1..242)
                     /locus_tag="PKN90_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ86060.1"
                     /translation="MRNTLLIVAFAIVALIFSIWGYNALRANSVSGKIDVYTAVPMQP
                     IAMLHINNIAELQSSLLYNNTYWLDVSKLEAIAPMHA"
     gene            complement(267..>1013)
                     /locus_tag="PKN90_07005"
     CDS             complement(267..>1013)
                     /locus_tag="PKN90_07005"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNZ86061.1"
                     /translation="TANVILAGGATMEVRLSRNLRFTSDLIFNHYSNGNTVQPNMGLN
                     LANVYAGFKYIPSYDGYVARGLARVPSTHDRFSGEVIFSGGSRQRYYKDTKSFPIASL
                     NLGGYYHTCHQHRIGIGLDGFYNGIFTNDPALSNSFRRTNITTDELKNKLRLGINLAN
                     DVLVGRFTAGLQFGVYLYDPIKDLEPYNPASRKDKGLIYAYDIEKEDGWLYTRLSAKY
                     RIIDHIIANISIKTHLHKAEFIEFGLGYAF"
BASE COUNT          301 a          192 c          209 g          311 t
ORIGIN      
        1 gcatgcatag gagctatagc ttccaattta cttacatcaa gccagtaagt attgttgtag
       61 agcaacgagc tttgtaattc ggctatatta ttaatgtgca acatagcaat tggttgcata
      121 ggaacagcgg tatacacgtc aatttttcct gatacagaat tggctctcaa ggcattgtat
      181 ccccaaattg agaaaattag agcaactatg gcaaatgcaa ctattaataa ggtatttctc
      241 atgtgtgttt attttcttta tgtcaattaa aaagcatacc ctagcccaaa ctcaataaat
      301 tcagccttgt gtaagtgcgt tttgatactt atgttcgcaa taatatggtc gataatgcgg
      361 tacttagcac taaggcgtgt gtatagccaa ccgtcttctt tttctatgtc ataggcataa
      421 atcaagcctt tatctttgcg gctggcaggg ttatatggtt ctaaatcttt aatagggtcg
      481 tataggtaca ccccaaattg tagcccagca gtaaaacgac ctactagtac atcattggca
      541 aggttaattc ccaatcggag tttatttttt aattcgtctg tagttatgtt agtgcgtcta
      601 aatgaattag acaaagcagg atcgttggta aatatgccat tataaaaacc atctaaccct
      661 atacctatac ggtgttggtg gcaggtgtgg tagtaaccac ctaaattgag agaggcgata
      721 ggaaaacttt tggtgtcttt gtaatagcgt tgacggctac ctcccgaaaa aataacttcg
      781 cccgaaaatc tgtcgtgcgt actaggtaca cgagctaatc cgcgtgccac ataaccatca
      841 tagcttggta tgtacttaaa accagcatat acattggcaa gatttaagcc catattcggt
      901 tgcacggtat ttccgttaga gtaatgattg aagataaggt ctgaggtaaa acgtaggttg
      961 cgtgataaac gcacttccat agtggctccg cctgctaaaa ttacattggc ggt
//
LOCUS       DAOPUR010000437         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_181101_length_1007_cov_2.650210, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000437 DAOPUR010000000
VERSION     DAOPUR010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181101_length_1007_cov_2.650210"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..>1007)
                     /locus_tag="PKN90_07010"
     CDS             complement(289..>1007)
                     /locus_tag="PKN90_07010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ86062.1"
                     /translation="MTVKLYNETPLHRDNFIKLVEQGFYNDLLFHRVINGFMIQGGDP
                     QSKNAPKGKQLGSGDVGYTIPAEFVYPQYYHKKGALAAARQGDQMNPKKASSGCQFYI
                     VQGNKIGDAELQQTEQQAKHKAKQTLFYALADQHKENIKQLRINKDTAGLEKLQTELI
                     AQVEKELATQSFTLPETLKNDYKTLGGTPFLDNEYTVFGEVIDNLEIIDKIAVSKTLS
                     GDRPEKDIKMTLTILQKGNY"
BASE COUNT          227 a          167 c          218 g          395 t
ORIGIN      
        1 atccactact tcgttggcat ttacttggtt gagtgtttga ttgtaagcct tttggaggtc
       61 ttctttgcta atttctgtgt aggtagcacc ttgttcgtat gctccccagt cgaaactctc
      121 atcaggttga ataaattctt ttgtcatgtt ttttgtttcc taattagttg ttagacatta
      181 tgttgattaa aaatgagccg caaaggtaat ggtttatttt gagataaccc acttttttgc
      241 aaaaattttt agtgtaaaaa tgctgcaaat agttgactgc ttgattcttt agtaattccc
      301 tttttgtaga atggttagtg tcattttgat gtccttttct ggtcggtcgc ctgatagggt
      361 tttgctaact gctattttat caataatctc taggttgtcg attacttcgc caaaaacggt
      421 gtattcattg tccaaaaagg gtgtaccacc cagcgttttg tagtcgtttt tgagtgtttc
      481 gggcagggtg aatgattgag tagcgagttc tttttctact tgagcaatta attctgtttg
      541 tagtttttct aagccagcag tatctttgtt tatacgtagc tgtttaatat tttctttgtg
      601 ttggtcggca agggcataaa acagtgtttg tttggctttg tgtttcgctt gttgttctgt
      661 ttgttgtagt tcggcatcgc ctattttgtt gccttgtaca atataaaatt gacaacccga
      721 tgaggctttt ttaggattca tttggtcgcc ttgacgagcg gctgccaatg ctcctttttt
      781 gtggtaatac tgtggataca caaattcggc aggtatggta taacccacat cgccactacc
      841 aagctgtttg cctttgggtg cgtttttcga ttgagggtcg cctccttgaa tcataaatcc
      901 gttgataacg cgatgaaata ataggtcgtt gtaaaaacct tgttcaacta atttgataaa
      961 attatcgcgg tgtaggggag tttcgttata taattttacg gtcatat
//
LOCUS       DAOPUR010000438         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_181309_length_1006_cov_4.033649, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000438 DAOPUR010000000
VERSION     DAOPUR010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181309_length_1006_cov_4.033649"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..257
                     /locus_tag="PKN90_07015"
     tRNA            184..257
                     /locus_tag="PKN90_07015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:Arg,seq:tct)
     gene            complement(331..1006)
                     /locus_tag="PKN90_07020"
                     /pseudo
     CDS             complement(331..1006)
                     /locus_tag="PKN90_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868490.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
BASE COUNT          331 a          201 c          158 g          316 t
ORIGIN      
        1 tgggggatta aagcatatga cccatcaaaa ataggttctg atgcttggcg gttttcgagt
       61 aattggaaca ttatgaaaga tacagcactc cattttgctg taggctatcc tttctaataa
      121 aattaaaact ataaaaaatt ggcaaagaaa aaaatttttt ctacctttgt gcccacaaaa
      181 cgaggttccg tagctcagct ggatagagca acgcccttct aaggcgtggg tcaagcgttc
      241 gaatcgcttc ggaatcacaa aaaaacgaga taaagaaact aactgcttta cctcgttttt
      301 ttggtatata tacctatata ttaatcacaa tcagagttta tactctgcaa tgataccact
      361 tccaacgcat atttcacatt tctcatcgta taatgcacca aactgacctg gagcaatacc
      421 ctgaattttt tcttcagatt caatacaata agtatttccc tttctatgta ttgtaccaaa
      481 agtaaactcg ggagtatgac gaattttaaa ggctacgcga gtgggattgt cgtatatagt
      541 tggattacct gatataatat gtaaatcttg aagtggaatc atcataccat actgtgtaac
      601 aacatcatat ccattagaaa catacacaat attttcatcc acctgttttg cgaccacata
      661 ccatggacct ccacttaatc ccaatccttt tcgttgtcca atagtatgaa accaaacgcc
      721 attatgctga ccaactactt ttcccgtttc gtattcaata atatgccctt ttttaactcc
      781 tacatatcgt tttataaaat ctgaataatt gattttccct aaaaaacaaa tcccttggct
      841 atcctttcgg gtagcactag gtaattgttg aagagaagct atctctctaa cacgagattt
      901 tggcaaatca ccaataggaa aagagattta gaaagttgct cgtatgaaat acgagataaa
      961 aaatacgttt gatctttatg tttatcaaca gctgtactca aatgat
//
LOCUS       DAOPUR010000439         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1413
            NODE_181360_length_1006_cov_2.788644, whole genome shotgun
            sequence.
ACCESSION   DAOPUR010000439 DAOPUR010000000
VERSION     DAOPUR010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854618
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:24:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,405
            CDSs (total)                      :: 1,390
            Genes (coding)                    :: 1,365
            CDSs (with protein)               :: 1,365
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 8 of 25
            Pseudo Genes (internal stop)      :: 8 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181360_length_1006_cov_2.788644"
                     /isolate="AMR_MDS_1413"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..>1006)
                     /locus_tag="PKN90_07025"
     CDS             complement(334..>1006)
                     /locus_tag="PKN90_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNZ86063.1"
                     /translation="IRKCVQAKKPVIVATQMLHTMIHNPRPTRAEVTDIANAIYYRTD
                     ALMLSGETAYGKYPVEAVRTMATIAKEAEKTKMAENDIPIEISTNDTTSFLCNAAVSA
                     CSNLGSKAIFTYTYSGLTARYLAAYRGQNPIFAICYHERSTRLLALSYGVFPFFQDSG
                     NDNTQQYIIKGLQFLLNEKWININDTVCYLSGSFGIGFGTTFLEINTVKKIFESREKY
                     ILPNF"
BASE COUNT          286 a          164 c          216 g          340 t
ORIGIN      
        1 accaaatcgg gtaattacgg gcaacagttt gaaattatgc aaaataaggc tagctaattt
       61 catctgtgga gtaaaatgta tctgtttcat tggcaaagga ataagtatat tatgtaggca
      121 attaactcat acaaagatag gcgttttttt tagaaatcgt ccgcttgcaa tcagaaaatt
      181 gtaggtaaac gaaccactag tcagtaatgg agatatatcc tacgcggaat atgcatacct
      241 ttgtattata gtttatctag tttgtttgta gcaaaaaaga ttctctttta tcctctaaaa
      301 cgtctatcta gttttgtttg taaagtagtg attttagaag tttggtaaga tgtatttttc
      361 tcgtgattca aatattttct ttacagtatt tatttcgagg aaggtagtgc caaaaccgat
      421 accaaagctc ccacttaaat aacatacagt atcgttgatg ttaatccatt tttcgttaag
      481 tagaaactgt aatcctttga tgatgtattg ttgcgtgttg tcgttaccac tatcttgaaa
      541 aaatggaaaa actccatagg aaagagctaa taatcgggta gatcgttcgt gatagcagat
      601 agcaaaaatt gggttttgac cacgatacgc tgccaaatag cgagctgtaa gtccgctgta
      661 ggtgtaagta aaaatggctt ttgaccctaa gttagagcat gctgaaacag cagcattgca
      721 taggaaagat gtggtgtcgt tggtgcttat ttcaatagga atatcatttt ccgccatttt
      781 agttttttcg gcttctttgg ctattgttgc catggtgcgt accgcctcta ccgggtattt
      841 gccataagct gtttcgccac ttagcattaa cgcatccgtg cggtagtaga tagcgttggc
      901 aatatcagtt acttcggcac gtgttgggcg aggattgtgt atcatggtgt gtagcatttg
      961 cgtagctaca atcactggtt ttttggcttg tacacatttg cgtatt
//
