LOCUS       DAOPUT010000001       102969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_166_length_102969_cov_19.450104, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000001 DAOPUT010000000
VERSION     DAOPUT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 102969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 102969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102969
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166_length_102969_cov_19.450104"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1349
                     /locus_tag="PKK75_00005"
     CDS             3..1349
                     /locus_tag="PKK75_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82004.1"
                     /translation="MVRPPRIKQAITLSDFMFSEPLGLEMIYGILKDDHEVEIFDMII
                     EAESLTNKVNEYMPEFVGITSLCIDVIKVLDLCRETKIANKSIITAVGGTQAYLGPES
                     FYNENVDYIFQYTTKDNIKKFFASLNGQGPVPIDGISSRVSSYKSTGQKGRNEYMLPD
                     RDSTKKYRNEYSYFGYKPAAIMEFGTGCEKACSFCLRWRIEGAEEKLIDIELNKNDLR
                     NIKEPAIMFIDNDFLCSEEKIVAFIDIVKELDLKKNFIMYGSTKGVIQYEKYLKLLSE
                     MGLKALLVGYETYSDDELSKYHKSSSADDNFKASDILRKNRIDVWASFMAHPDWDKND
                     FRSLRKYIKKLKPQITTISPLTPFPNLPMFKEFEDRLLFSREDYERWSFGQVMIKPSK
                     ISLKDYYYELLKTNLYVNVFINKNTEMIKKYGIINIFRILKGSLKAVKKYMALMNE"
     gene            complement(1372..2439)
                     /gene="rsgA"
                     /locus_tag="PKK75_00010"
     CDS             complement(1372..2439)
                     /gene="rsgA"
                     /locus_tag="PKK75_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNZ82005.1"
                     /translation="MSKINLKDYGLNDRFINESQMYNFSLGRIISQDKGLYTAVTENG
                     EFKAQISGKLRYNAVNISDYPAVGDFVMLDENEGTSVIHHILTRKSAFIRKAAGTSKD
                     DQVVAANVDTVFICMSLNRDFNLRRLERYIAIGWDSGAIPVVVLTKSDLCTDIDSIFL
                     ELSGIAIGLDILVTSSLAEEYESIKKYINYGCTVAFIGSSGVGKTTLINKLMGQSIFE
                     TREIRNDDRGRHTTTRRELILLPEGGIVIDTPGMRELGIESADFSRAFSDIDELSQMC
                     KFSDCTHTREPGCAVKKAVESGSLSEERLESYFKLKKEADYEGLNFKQIETQKLNRMF
                     KDVGGMKNIRRFSKEKNKYNE"
     gene            2716..2889
                     /locus_tag="PKK75_00015"
     CDS             2716..2889
                     /locus_tag="PKK75_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82006.1"
                     /translation="MDKNFEEIKEEFIKASLDEKIKIYSTTQGLTVEQFKELLAYFPI
                     KYLDKLEEAVNSL"
     gene            3060..3959
                     /locus_tag="PKK75_00020"
     CDS             3060..3959
                     /locus_tag="PKK75_00020"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNZ82007.1"
                     /translation="MIKDNKLRKTFIKEALGSRNYLSYRTDLMLLKIIASFAVFITVY
                     FIYLDLLLSLLTALLVFAVFTMTNKVNVDKKNEKGRELLFKKAKKEYFLSKIEEINKS
                     DFEMLIKLFFKNEGYNNIIKKGKGLYLAEKEGYITCIKVFKLFEGIEVEKLDIRSMLT
                     FMGNSNIKNGFLVTTSDLSEDAAKLIEKFKDRYEITIIDIEGMYNLADKYNMLPEDSF
                     YYKRMSEEKITTKKNVRENVLSINKIYLYIAAAIFFYMSSVWLPENKLVIYISYFFII
                     MTIINVFYFLANRYSNKKFNKVE"
     gene            4021..4779
                     /locus_tag="PKK75_00025"
     CDS             4021..4779
                     /locus_tag="PKK75_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HNZ82008.1"
                     /translation="MRDNFMVQKQLEHRTIREFKNESIPKDLFEQLIEVAKRTPTSNG
                     MQASSIIRVTDPEKKKEIAAVCKQEYVARVPELLIFVADQYRNYRIADDKGGYEPSAR
                     DMDRFFQGFTDACLTAQNIVNAAEASELGVVFFGSILNDAERIIEILELPELTMPIVG
                     LGIGYPNQDPQLKPRMSMELRLFENTYKKFDNYVEVIKEYDIEMQTYYDLREANKRVD
                     SFSNQVVTRLKNLIPKRSDMLKIMKKQGFDLNTD"
     gene            4950..5528
                     /locus_tag="PKK75_00030"
     CDS             4950..5528
                     /locus_tag="PKK75_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HNZ82009.1"
                     /translation="MKFIATVGLSNKHVHLSKEHINILFGEGHELTPMKDLSQPGQYA
                     CEEKVDLVGPKRTIKGVRILGPARKETQVEVSFSDGFTLGLEEVPVRDSGKTEGTPGL
                     KLVGPKGEVELDKGVIAAARHLHMHTSDAEKYGLKDKDTVSVKVNGPRGLTFNNVLVR
                     VNPEYALDMHVDIEEGNAAGLKNGDEIEVMID"
     assembly_gap    5555..5654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5967..7760
                     /locus_tag="PKK75_00035"
     CDS             5967..7760
                     /locus_tag="PKK75_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNZ82010.1"
                     /translation="MDFLPVNIEDMKKRGWDECDFVFVTGDSYVDHSSFAVGILSRLL
                     ERFGYKAGIIPQPDWKNLSSFRVLGKPRLAFLVNSGNIDSMVNHYTVFKNKRRSDAYS
                     PGGKAGLRPDRAVTVYSNRIREAYKDVPVIIGGIEASLRRFSHYDYWSDSVKRSVLLD
                     SGADLLVYGMGEKALIEIAQALESGIPIEEITFVNGTVYKTKDRDRPYSPIFLPSYNE
                     VVSSKKKFGDSFKIQYENTDSKSAGTLVEQYDTVYVVQNPPMEILSQQELDDIYDMDY
                     TMNYHPMYKNSGIPALNEVKFSITSVRGCFGGCSFCSLNFHQGRIIQKRSHESVLNEV
                     EKMRRDKDFKGYIHDVGGPTANFRIKACKKQEKYGACIDKECLTPERCKNLQVDHCDY
                     IKLLRKIRNTEGIKKVFIRSGIRYDYVIYDNNDEFMEELCTHHVSGQLRTAPEHVSEH
                     VLKLMGKPSIKIYNKFVKKFYEINKSIGKEQYIVPYFISSHPGSRLNDAIELAEYIRD
                     MGHMPEQVQDFYPTPGTLSTCMYYTGFNPITNEEVYVPRDKEERKMQRALLQYSKKEN
                     YELVLKALKMENRYDLIGYGPKALIKPKKMP"
     gene            7921..9189
                     /locus_tag="PKK75_00040"
     CDS             7921..9189
                     /locus_tag="PKK75_00040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNZ82011.1"
                     /translation="MNRKARRRKKRIRLMSWSLVLLVGVLAASFVVKGMGKDRKNEEQ
                     PPADDPVNEQIGEETSNEVTKPLPEPDVSIRIRATGDIMFHPSQLEGAYDYATNTYNF
                     ENSFKAIKNIIEEADIAIANFEGTAAGNGTYAYQGYPLFNAPDEALDAIKNAGFDILQ
                     TANNHSLDTRKAGIIRTIEQIKARGMEPAGTFREKPETRVLIKEVKGIKLAFIAYTEM
                     VNGLESVLSPQDLDAMVNILNESKMKEDVAFAREQNADAIIALFHWGDEYSRVQAKRQ
                     EILADMLFREGVDIILGNHPHVIQPTQTLEYDGKTKFVAYSMGNFLSNQRVETLVPYG
                     LTEHVSKYTEDGIIIDIEIEKKAGTQEVSIKNINYIPLWVYKGFKENGTAEHVVYPIM
                     DYIEGAELDEDSKARMSRSYADTMAQMNGE"
     gene            9203..10039
                     /gene="cdaA"
                     /locus_tag="PKK75_00045"
     CDS             9203..10039
                     /gene="cdaA"
                     /locus_tag="PKK75_00045"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNZ82012.1"
                     /translation="MELLDRIRLIVTNVGLNTIVDIGIVYYVLYRGYILIKDMRAKQL
                     VKGIVLLVALIPISSIFDLYMVEFILRNTFQVGLIALVIVFQPEIRKALEYIGRTSFT
                     ISNVEKNTETSKKIIKEIISAATSLSRQKIGALIVFEKQIGLNDIIESGTVINANISS
                     GLLINIFIPNTPLHDGAVIIKDYIIKAAGCFLPLTDNNLLSKDIGTRHRAAIGITERS
                     DSIALVVSEETGYISYAVEGRLYRNIQAEEIETILHSIYIENEKVKIIDKWRNRNEDK
                     RR"
     gene            10020..11255
                     /locus_tag="PKK75_00050"
     CDS             10020..11255
                     /locus_tag="PKK75_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNZ82013.1"
                     /translation="MKIKDDKLVIQIACLVVAIVLWMIVMLVTNQTLEDSYPNVSVTI
                     RNLSALENSNLVIMNSDKENITVTVKATGLTEQLNNISSRDFSAYIDVLGFKEGITNA
                     KVEVVGPSGVDIVSVYPAQIACNIEGVISKVMDVTVQSEGVQAGSYYKAQTVSNPSSV
                     KITGPRSVVNSAEKAVATININGATDDVIKTVPVRVYDDTDTEIFLSAPTGNVKVTVP
                     IYPTKYAELVPGVTGTPEEGYELVDVTVKPERVRIAARQDILDTIKELNLEVIDITGA
                     YNNMLTARNILNAEGLILLDLEAAPVVNTVIEKIIQREFKYTYDDIQLINKSENTDAV
                     IEETDEFLTVTVFGTTTLLTEMEKDDLILTADLSETVTGLNTVNIDYVSEFTFKEIIL
                     SKGTLNLEIIEAVEPQEEE"
     gene            11259..12641
                     /locus_tag="PKK75_00055"
     CDS             11259..12641
                     /locus_tag="PKK75_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ82014.1"
                     /translation="MQKINFNSMDYQGMLEAVLQAAQDAISVVDEKGEHILVNPAYTR
                     ITGIDYDEIIGKNALYDIEDGEQSVHMKVLSTKKPVENTKLKLRPSGKTVVAQAAPIN
                     VDGEMKGSVAVLHDYSTIKDLTDKLEEAEKKLRQLTYKYKPEDIIGNSKAINEVRNLI
                     KKAAKVPATVLLRGESGTGKELFANSIHAQSTRSRKNFIRVNCAALTDSLLESELFGY
                     DEGSFTGALKGGKKGLFEEADKGTIFLDEISEISLNTQAKLLRVLQEKEIMRVGSSKN
                     IPVDVRVIAATNSDLTKLVSEGKFREDLFYRISILPISIPPLRERPEDIPLLVKSFIV
                     KFNDEYGRNIIDITDDALAVLKSHDWPGNVRELENIIGRAIIYMNLNERIIDRAHLPH
                     LNANKQNTGIAVTIDEGVQDLESILDKTENEYITKVYLKFNKNKTRTARELGISLRSL
                     YYKLEKYGIL"
     gene            12807..13712
                     /locus_tag="PKK75_00060"
     CDS             12807..13712
                     /locus_tag="PKK75_00060"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HNZ82015.1"
                     /translation="MYRFDKLLKESGNTQPKKIAVAAPHDDDILKAVKSAVLKNICIP
                     ILIGIKDKIIELSKEINFNLDNIEIIETNDDISAARTAVSLVSSGKADIVMKGLLDTS
                     IILKAVLDKEIGLRTGSVLSHAAVFETDKYHKFLIITDAAMNIAPTASEKKQIIENTL
                     PLCRSLNIENPKVAVICAKEKVSDKMQATLDAEILVNLYKAGEIKGCIVEGPFGLDNA
                     ISREAATVKGVKGEVAGDADVLLMPNIEAGNVMYKTLTYLADSKNAGIILGAKAPVIL
                     TSRADSDEAKLYSILLAVICSKESI"
     gene            13709..14776
                     /gene="buk"
                     /locus_tag="PKK75_00065"
     CDS             13709..14776
                     /gene="buk"
                     /locus_tag="PKK75_00065"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNZ82016.1"
                     /translation="MKQLIINPGSTSTKIAVFDDETMVFEKTIRHTNEELQQFNRVSD
                     QFQFRKDMIILALKENSISLDMLDAVVGRGGLIKPVEGGTYTVNDKLIEDLRKNVKVE
                     HASNLGAPLARSIADEVNIPSFIVDPVVVDEMEDVARVSGHPDFERISIWHALNQRAV
                     ARRAAMEQFGRKYEDLNFVVAHLGGGISIGAHKKGRAVDVNNALNGEGPFSPERSGSL
                     PMGQLVKMCFSGLYTEAQINKMMVGKGGISAHLGTNSAKEVSDRAQAGDEKAKLIYSA
                     MAYQVAKAIGEYAVVLDGDVNAILITGGIAYDEYFVKMIEDKVKFIAKVIAYPGEDEL
                     LALAQGGLRVLKGIENPKTYI"
     gene            14806..15483
                     /locus_tag="PKK75_00070"
     CDS             14806..15483
                     /locus_tag="PKK75_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ82017.1"
                     /translation="MMHDKRLVIVIGHYGSGKTEFAVNYAIKMKEIYDSVSIADLDIV
                     NPYFRSREKRELFEEIGIKLFDSSIRNTAVDIPALPAELMGVILNQNVKSILDVGGDP
                     VGARVLARYSDQIKNVEYDMLYVINGNRPSTSTVEGVLKYLKMIEDTSRLKITGLVNN
                     THMLKSTTVEDVEFGHELTKKVSWETDIPIRYEAVIKETADKIKNQEIIEKLFPINLY
                     MREEWMS"
     gene            15497..15712
                     /locus_tag="PKK75_00075"
     CDS             15497..15712
                     /locus_tag="PKK75_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ82018.1"
                     /translation="MAKGKVTFNDNACKGCELCVAVCPVKIIKLNKEKINTKGYSPAH
                     VIDQEKCIACGNCAIMCPDSVIKVERL"
     gene            15725..16786
                     /locus_tag="PKK75_00080"
     CDS             15725..16786
                     /locus_tag="PKK75_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNZ82019.1"
                     /translation="MSKILMKGNEAVGAAAIKAGCKFFFGYPITPSSEIPEYIARELP
                     AAGGVFLQAESEIAAINMVYGAGGSGARVMTASSSPGISLKQEGITYCAGAEIPCVIV
                     NMMRGGPGLGSIQPGQADYYQSTRGGGNGDYRTLCYAPASIQELVDYVMLAFDKADYY
                     RNPVLICGDGMIGQIMEAVEFKDPEPIELPPKDWATTGTGGKRKPVVVNSLYLEAEEL
                     QEHNKHLEKKYKQMEENEVLYEAFGLEDADVVCVAYGTTARIVKTAIKQAKAKGINVG
                     MIRPITLWPYPYDVFKEIGDKAKGILTVEMSQGQMTDDVKIGIAGRLPVYFNGTQGGM
                     VPTPGNILADIEKIAGGAK"
     gene            16786..17532
                     /locus_tag="PKK75_00085"
     CDS             16786..17532
                     /locus_tag="PKK75_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ82020.1"
                     /translation="MTIVYEKSKGLTDTPFHYCPGCTHGVIHKLVAESLVELGVLDDA
                     IGVAPVGCSVLAYKYFNCDMQEASHGRAPAVATGIKRVHPDKVVFSYQGDGDLASIGT
                     AEVVHAAHRGEKITTIFVNNAIYGMTGGQMAPTTLVGQKTTTSPFGRDEAHCGKPIKM
                     SELLATIDGAKFVERVTVDTPANIRKAKKAIKRAFECQIKGEGFAIVEVLSSCPTNWG
                     FTPLKALDWLRDNMIPYYPLGNFRDFGEGK"
     gene            17534..18076
                     /locus_tag="PKK75_00090"
     CDS             17534..18076
                     /locus_tag="PKK75_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNZ82021.1"
                     /translation="MEQKVIMAGFGGQGVMAIGKLLAYAGMIEGKNVTWMPSYGPEMR
                     GGTANCAVVVSEEEVGSPLISKDGTAAIVMNLPSMVKFEKELVPGGKLIINKSLIDVE
                     PERKDLDVYYIEANELALELGNSKVANMVMLGAYLEITKAVEIESVLQAFVKVFGEDK
                     RDLVPLNKEALLKGAQAAKK"
     gene            18214..19236
                     /locus_tag="PKK75_00095"
     CDS             18214..19236
                     /locus_tag="PKK75_00095"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNZ82022.1"
                     /translation="MEKKDIKWAELGFNYIKTDKRYISYWKDGKWDEGQLTDDNKVHI
                     SEGSTCLHYGQECFEGLKAYSNKDGGIQLFRPDENAKRMQKSCERVMMPIVPVEKFID
                     ACMQVVKANAAWVPPYGSGATLYLRPYVIGVGDNIGVKPAEEYIFGVFCMPVGPYFKG
                     GMNPVNFITTDEDRAAPNGTGKIKVGGNYAASLHSHFEAGKKGFADCIYLDPATHTKI
                     DEVGAANFFGITKDDKFVTPHSSSILPSITRISLMQVAKDYLNMEAVETEIYIDEIDN
                     LKEAGACGTAAVITPIGGIMHNGKLHVFHSETEVGPVTKKLYNTLYGIQMGDIEAPEG
                     WIYKVI"
     gene            19358..20446
                     /gene="queG"
                     /locus_tag="PKK75_00100"
     CDS             19358..20446
                     /gene="queG"
                     /locus_tag="PKK75_00100"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNZ82023.1"
                     /translation="MKQIIREAAYKTGIETFGISNTADYSYMKDMLNSRKENNQDCEF
                     EEQDINKRLNAKNLFPELKSIIAIGIPYGEGYKPPSSLNKGLLSVSCHGEDYHNTVTS
                     LLLKLAEEIKRHSKFEFLPVVDTSFLIDKEICKSAGIGNYGKNSLLINKKAGSFMNLG
                     YLLTDIEIPADTIKEEDICGTCILCVKACPNNAIFEKGGINPKRCVSYLTQTKSYIPL
                     EFRESMGRQIYGCDICQIVCPLNRDLLNNKTRDAYSDLEVDLEELLMISNIGFIKKYG
                     GSAGGWRGRNIWKRNAVIAAGNLKIYSMFQTIKSELQNPSDMIKMYAAWSLLKLNKPS
                     AENILYDNLKYENDNVKSEYLKLLEEKL"
     gene            20443..20724
                     /locus_tag="PKK75_00105"
     CDS             20443..20724
                     /locus_tag="PKK75_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNZ82024.1"
                     /translation="MIVCSCEIEILIYESNSLKEKRQVVKSIIERIRSKFNASIAEVG
                     YNELWNRSLLGLAVVSNNKRLCDASISKIISFIDNDERVEIINCRREMV"
     gene            20721..21356
                     /gene="nth"
                     /locus_tag="PKK75_00110"
     CDS             20721..21356
                     /gene="nth"
                     /locus_tag="PKK75_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNZ82025.1"
                     /translation="MMTKAEKVYELLKEAYPDAKCGLEYKTPLQLLISTILSAQATDK
                     SVNSVTEELFIRYPDLDSFLTLSQEDLEQKIKKIGLYKNKSQNIFNMLRVLKDKYNGE
                     VPKSMDELLSLPGVGRKTASVVLVEAFSIPAFPVDTHVFRVTRRIGLAVESTADKVSD
                     EMMKKLPKYKWHLMHHLFITHGRTTCTAQSPKCNICMVNELCKYFKKQDKQ"
     gene            21436..21906
                     /gene="trmL"
                     /locus_tag="PKK75_00115"
     CDS             21436..21906
                     /gene="trmL"
                     /locus_tag="PKK75_00115"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNZ82026.1"
                     /translation="MSLNIVLFEPEIPQNTGNIARTCAATGSKLHLIKPLGFSVNDKY
                     LKRAGLDYWNLVNITYYENFEEFKKTTGNNKVYISTTKESRFYTDVTYFDECYLIFGK
                     ETAGLPDYIHREYMQDRIKIPMIQKVNARSLNLANSVNIVIYEALRQLGFPGMI"
     regulatory      22246..22327
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            22385..22744
                     /locus_tag="PKK75_00120"
     CDS             22385..22744
                     /locus_tag="PKK75_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HNZ82027.1"
                     /translation="MIITNNDWVLYKYREKFNVVYIDGTFKDVLIAVRDRLHMGYELL
                     THPLGGSVKPGETPYKSIMVSDDKKSISFSSIYTIENAIITYDKFNKNKLKFINDKIA
                     EDLKLIDLTVLESALNI"
     gene            22777..24057
                     /locus_tag="PKK75_00125"
     CDS             22777..24057
                     /locus_tag="PKK75_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HNZ82028.1"
                     /translation="MKLELGYIFIKDIQFSDVSKVEDGTLYVNKEEVKALILEDEHFK
                     KAEVELAKPGESVRIMPVKDVIEPRVKVEGPGGIFPGMISKVETVGSGKTNVLKGAAV
                     LTTGKIVGFQEGIIDMTGPGADYTPFSKLCNLVLVCEPIDGLKQHEHEKALRFAGYKV
                     ASYLGGLAKKLTPDQVEVFETPTLTEGLKMYPGLPRVAYVCMLQSQGLLHDTYVYGID
                     AKQTLTTFIYPTETMDGAIVSGNCVSACDKNTTYHHLNNPVIADLFAEHGKTLNFVGV
                     IISNENVYLADKERSSNWSAKLAEFLGVDGVVVSEEGFGNPDTDLIMNCKKIESKGIK
                     TVLITDEYAGRDGTSQSLADADALANAVVSGGNANEVIELPPMEKIIGHVEVADIIAG
                     GFDGSLHKDGSITAELQVITGATNEMGFNELSAR"
     gene            24075..25379
                     /gene="grdB"
                     /locus_tag="PKK75_00130"
     CDS             24075..25379
                     /gene="grdB"
                     /locus_tag="PKK75_00130"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:25116..25118,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HNZ82029.1"
                     /translation="MLKIVHYINQFFANVGGEEMAHIPAEVREGVVGPGLAFQQAFKG
                     EAEIVATIVCGDSYFNENMDEAKSTVIKMVKEYQPDLFIAGPAFNAGRYGVACGTIAQ
                     AVKDELNIPVLTGMYAENPGADMFKNTIYIVSTKNSAAGMRDAVKKMAPLALKLAKGE
                     KIGASAEEGYMPNGKRVNFFEKDRGSKRAVKMLLAKLADKEFETELPMPDFDRVAPNP
                     AVKDITKAKIALVTSGGIVPKGNPDHIESSNASKYGKYDIDGVMDLTEETYQTAHGGY
                     DPVYANLDADRVLPVDVLREFEKEGKIGSLHRYYYSTVGNGTAVKNAKGFAAEYAKEL
                     KADGVDAVILTSTUGTCTRCGATMVKEIERAGIPVVHMCTIVPISLTVGANRIVPTIA
                     IPHPLGNPALSLEEERDIRRNLLTRALKALETEVTDQTVFEK"
     gene            25535..26470
                     /gene="trxB"
                     /locus_tag="PKK75_00135"
     CDS             25535..26470
                     /gene="trxB"
                     /locus_tag="PKK75_00135"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNZ82030.1"
                     /translation="MSKIYDVLIIGAGPAGLSAGLYAGRARLSTLIIEKEKDGGQIVL
                     TSEVENYPGCLEGESGPTLVDRMAEQAKHFGAEKVYDEILEVKLDGKEKVLVGKKGEY
                     VGKTVIIAGGATSKPIGCPGEKTFTGKGVSYCATCDASFFEDFEVFVVGGGDTAVEEA
                     MYLTKFARKVTVIHRRNELRAAKSIQQKAFANPKMNFMWDSVVVEIKGDGLVNSMVVK
                     NVKTGELTEIVADEEDGTFGIFGFIGYEPKTKIFEGILNIQNGYLVTDEDMKTNIEGV
                     YAAGDIRVKSLRQVVTAAADGAIAAVQAEKYIESL"
     gene            26484..26801
                     /locus_tag="PKK75_00140"
     CDS             26484..26801
                     /locus_tag="PKK75_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNZ82031.1"
                     /translation="MLLLDKETFEPEVLKAEGYVFVDFFGDGCAPCEALMPHVMALSE
                     KYGDKLKFTKLNTTKARRLAIGQKILGLPVIAVYKDGEKVEELIKEDATPENVEAMIQ
                     KYI"
     gene            26866..27339
                     /gene="grdA"
                     /locus_tag="PKK75_00145"
     CDS             26866..27339
                     /gene="grdA"
                     /locus_tag="PKK75_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_145084612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:26995..26997,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HNZ82032.1"
                     /translation="MSLLQGKKVIIIGDRDGIPGPAIEECVKTAGGEVIFSSTECFVU
                     TSAGAMDLENQKRVKEFAEKYGPENVVVVLGASEGEAAGLAAETVTNGDPTFAGPLTG
                     VQLGLSVFHVVEPEIKDIVDPAVYDEQVGMMEMVLDVDDIINEMAPIREEFCKYL"
     gene            27404..28936
                     /gene="grdC"
                     /locus_tag="PKK75_00150"
     CDS             27404..28936
                     /gene="grdC"
                     /locus_tag="PKK75_00150"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HNZ82033.1"
                     /translation="MNSVIKGASYILAFTPDMVIHNGTTQTTERTVNPNSEYLKQIKD
                     HLRTFEEVVNYMPNQAYIGNMTPEELGQYEQPWNDKPLQGASRWGKFGEIMPQDEFIV
                     LMQICDVFDLVKLENDFLAETTAKLKNHPLFDEALLARIKQGEDGELIKKLVEEEHSE
                     ALYHNGQLVGCVKRAHDIDVNLNAHVMLENLVSKASNVLALLNLVAKNNINKDEIEYV
                     IDCCEEACGDMNQRGGGNFAKACAEVAGFNNATGSDTRGFCAGPAHALIEAAALVKAG
                     VYKNVVVSAGGATAKLGMNGKDHVKKGLPILEDVLGGFAVLISENDGVNPEINLDLVG
                     RHTVGTGSSPQAVITSLVSSLERAGMKITDIDKYSVEMQNPDITKPAGAGDVPAANYK
                     MIGALAVKLGQLDRKDLAAFADKHGMNGWAPTQGHIPSGVPYLGFGRQDILDGKIKNA
                     MIVGKGSLFLGRMTNLFDGVSFVIEANKGKQEPQSTISEEKVKELIAQALKGFASNLM
                     AE"
     gene            28947..>29454
                     /locus_tag="PKK75_00155"
     CDS             28947..>29454
                     /locus_tag="PKK75_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HNZ82034.1"
                     /translation="MSEKQVNQIIGKVFNQLADTLISGEYGQKPRVAVTALGSEHGED
                     EVYKAAAGTLKYGIEPSVIGTKADERVKAVTVASEDDAHKKMEELLDSGSVDAAVTMH
                     YPFPIGVSTVGRIVTPGKGKEMFIATTTGTSSTDKVEGMVKNAVYGIITAKACGIKNP
                     TIGILNVDG"
     assembly_gap    29455..29554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29555..30277
                     /gene="grdD"
                     /locus_tag="PKK75_00160"
     CDS             <29555..30277
                     /gene="grdD"
                     /locus_tag="PKK75_00160"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HNZ82035.1"
                     /translation="AKACGIKNPTIGILNVDGARQTEIALNKLKNNGYEINFAQSGRA
                     DGGAVMRGNDLLTGSCDVMVMDSLTGNLMIKIFSSYTTGGSYESLGYGYGPGIGENFD
                     KLILIISRASGAPLIEGAMRFAGDLIKGNCLQVAKEEFGKAKAAGFDAILSEIKASKK
                     PAAEEAEVAAPPKEVVISQISGVEIMDLEDAVKLLWKNGIYAESGMGCTGPIILVSEA
                     NTNTALKVLADNEFIAKEQLEC"
     gene            30437..31831
                     /gene="rpoN"
                     /locus_tag="PKK75_00165"
     CDS             30437..31831
                     /gene="rpoN"
                     /locus_tag="PKK75_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNZ82036.1"
                     /translation="MKMGFELNLSQTQKLIMTPELRQAIQILQFNNVELTDFINKQLE
                     VNPFLESVDNKSDASSNDEPAVENKESEDIDWRELAEKYDDFSYNTYSYADGENKQSF
                     ESYTSRKLSLKDHLMVQLGMSVKTNKDKRIGEFIIESLDNKGYLGCSLQDISLLINEN
                     IVDVEKVLRLIQTFDPVGVASRDLSECLMIQLKEKGIQDKNAYIIAEKYLEDIATNKI
                     QKIAKELHISVRRVQNIFDIIKMLEPKPSRGFIVDSDNIRYIVPDATIEKINDEYIII
                     INDSYLPTLTINRYYKNMINELEDKEASKFLTDKLNSSMWLIKSIEQRRMTLYKVIES
                     ILKFQRKFFDEGKSALKPLVLKDVADDIGMHESTVSRATNGKYVQTPRGIYELKYFFA
                     SSVSETDGSMVSSTSVKSIIQKLIDQESSQKPLSDQKITEILNEEGINISRRTVAKYR
                     DDLRIPSSAMRRRY"
     gene            32012..33019
                     /gene="gap"
                     /locus_tag="PKK75_00170"
     CDS             32012..33019
                     /gene="gap"
                     /locus_tag="PKK75_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNZ82037.1"
                     /translation="MVRVAINGFGRIGRLALRLMAQDEEFQVVAINTNSDAETLAYLL
                     KYDTAHGNYMTDKISYKDNIMYVNGKEIITSRVKEPENCNWKELDIDLVIDCTGKFNE
                     KDKAVAHIKAGAKKVLLSAPGKGDLKTVVYNVNHNILDGSETVVSAASCTTNCLAPLA
                     KVLNEEFGIVKGLMTTVHAYTNDQNIHDNSHKSGIRARRGRAAAANIVPASTGAAKTV
                     GVVMPELKGRLDGIALRVPTLTGSVVDLTVELGRNVTVEEVNQAVKNAANETLTYTED
                     PIVSSDVIGSHYGCIFDALSTQLIEADGKQLVKLIAWYDNEMSFTAQLVRLAKYWGSM
                     K"
     gene            33086..34270
                     /locus_tag="PKK75_00175"
     CDS             33086..34270
                     /locus_tag="PKK75_00175"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009529736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNZ82038.1"
                     /translation="MNKKTLKDLNFENKKVLVRCDFNVPLDKEGNITDDIRIVSSLPT
                     INYLLDKGASLILMSHLGRPDGAPDKKYSLKPVAERLSTLLEKNVLFEDSDQVVDDTV
                     KANAAKLKVGDLMLLQNTRFRKEETKNAGTFAKDLASLGDLFVNDAFGTAHRAHSSNA
                     GICEHLPSALGLLVEKEVEIMGNALKEPKRPLTAILGGAKVSDKIAVIENLLNIADNV
                     LIGGGMMYTFLKAKGFNTGSSLLEEDKVDLAKSIIEKSAEKLLLPLDTVVAKEFKNDT
                     EFFTTDADKIPDGYMGLDIGSKTVEMFTEIINNSKTIIWNGPLGVFEMDNFANGTNSI
                     AKAIADNKDAISIIGGGDSAAAVEKIGLADMITHISTGGGASLEFLEGKVLPGIEAVD
                     NK"
     gene            34334..35080
                     /gene="tpiA"
                     /locus_tag="PKK75_00180"
     CDS             34334..35080
                     /gene="tpiA"
                     /locus_tag="PKK75_00180"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNZ82039.1"
                     /translation="MRRKLMAGNWKMNNDVEESLKLVKSLKDSPLNSDVDVLICPPFT
                     SLYPVILELKNSSIKVGAQNMHFEDSGAYTGEVSPTILKNLGVEYVIIGHSERRQHFG
                     ETDQFINKKVKSALNHGLKPILCVGESLEQREEGIEKETVRTQLIMDLEGITEGEKIV
                     VAYEPIWAIGTGRTATSAQANEMSAFIRAVLGDIFTKDTAQNIIIQYGGSVKASNAKE
                     IMLQSDIDGALVGGASLKADEFIKIINFEN"
     gene            35092..36618
                     /gene="gpmI"
                     /locus_tag="PKK75_00185"
     CDS             35092..36618
                     /gene="gpmI"
                     /locus_tag="PKK75_00185"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNZ82040.1"
                     /translation="MNKVTALIILDGWGIGPDYEGNAIKRAKTPNFDVLLRRYPNTTL
                     SASGYDVGLPKGQMGNSEVGHLNIGAGRIVYQDLTRITMSIEDGEFEKNEALLGAINY
                     AKENRSSLHLIGLLSDGGVHSHNTHLYSLIELAEKNGLSDVQIHCITDGRDVSPTSSP
                     NYIKELMEKTKKAGTGRIASIMGRYYAMDRNRQWDRVELAYNALTKGEGEIFKDPVEA
                     VRNSYLTGITDEFIKPILIEKENGGLGTIKDNDAVIFFNFRPDRAREITRAFVDDDFS
                     YFNREKLNVKYVCMTMYDKTIKNVEIAYKPNFVRNTLGEYLSSLGIKQLRAAETEKYA
                     HVTYFLNGEIEEPFENEVRILIPSPDVPTYDLKPEMSAYELKDTVMEQLDKDIYQVMI
                     INFANPDMVGHTGDIEAAVKAVETVDKCMGEIVNFIIRAEGTAIITSDHGNCEEMFDK
                     ETLDTFTAHTVNRVPFIVVSKKNLNLKEGILADVAPTMLDLMGLKKPHDMTGNSLIEI
                     "
     gene            36631..>37037
                     /gene="eno"
                     /locus_tag="PKK75_00190"
     CDS             36631..>37037
                     /gene="eno"
                     /locus_tag="PKK75_00190"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150542.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNZ82041.1"
                     /translation="MSIITDVYAREILDSRANPTIEVEVWTESGGFGRAGVPSGASTG
                     AFEAVELRDGDKNRYLGKGVLTAVDNVNNIIAPELIGRDSLNQLEIDKLLIELDGTPN
                     KGKLGANATLGVSMACARAAADTLGLPLYQYLGG"
     assembly_gap    37038..37137
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37138..37650
                     /gene="eno"
                     /locus_tag="PKK75_00195"
     CDS             <37138..37650
                     /gene="eno"
                     /locus_tag="PKK75_00195"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060554.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNZ82042.1"
                     /translation="EGRVFTQEEMVEYYADLVDKYPIISIEDGMNEEDWEGWKMLTEK
                     LGDKIQLVGDDLFVTNTVRLKKGIELKVANSILIKLNQIGTITETLDAIEMAKRAGYT
                     AVVSHRSGETDDSTIADLVIAVNAGQIKTGAPSRMDRVAKYNQLLRLEDILGDTAQYK
                     GLDVFYNIKG"
     gene            37868..38098
                     /gene="secG"
                     /locus_tag="PKK75_00200"
     CDS             37868..38098
                     /gene="secG"
                     /locus_tag="PKK75_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNZ82043.1"
                     /translation="MEIVVRILLIIASLILIASILLQSGKSAGMSGEIAGGAESLWGK
                     NKGRSYEGKLEKATTAAAIIFVIASLILVAIQ"
     gene            38217..40217
                     /locus_tag="PKK75_00205"
     CDS             38217..40217
                     /locus_tag="PKK75_00205"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNZ82044.1"
                     /translation="MDTLIIAPVVGVLALLFAFYKASVVSKADPGTDRMQEISSYIHE
                     GAMAFLARQYKSVSIFAIVMFIILGIAINLPTAAAFAAGAACSAFAGYFGMTVATKAN
                     VRTTNAAMTSGMGKALDIAFSGGSVMGMTVVGLGVIGTGIFWMIFKDATVVTGFSLGA
                     SSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGMG
                     ADLFESYVGAIVSAITLGVISFQGGDGVLFALLVSAVGIIASILGTAFVRGESNPQKA
                     LNMGTYASSAIVIAASFFLSKSILGSYQPFGAIIFGVFVGLIIAKITEIYTSDEYKYV
                     KEIAAQSETGAATTIISGLSVGMRSTAFPTIVLAIGIIGAYAIAGYTPEGVDAVRGLY
                     GIALAAVGMLSTTGMTIAVDAYGPISDNAGGIAEMCELPHEVREITDRLDAVGNTTAA
                     IGKGFAIGSAALTALALFASYTQAVGITSISLTEPKVIAGLFIGGMLPFLFTAITMSA
                     VGKAAFSMIEEVRRQFREMPGVMEGTTKPDYKKCVDISTQAALKEMVVPGLLAVIVPL
                     AVGFLLGKEALGGLQAGALVTGVLMAVFMSNSGGAWDNAKKYIEGGKHGGKGSHAHQA
                     AVVGDTVGDPFKDTSGPSINILIKLITVVSLIFGSLLGNGII"
     gene            40330..41097
                     /locus_tag="PKK75_00210"
     CDS             40330..41097
                     /locus_tag="PKK75_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018924383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ82045.1"
                     /translation="MYIDIDGLNINYTDEGNGKSVLLLHGWGGSIQTMMAIGNILKDK
                     FRVISVDLPGFGKSGIPEVPWDSYDYAECIYKFIQKLNLSGLIVFGHSHGGRISIILA
                     SKYEVVRKLILIDSAGIIPKRKAKYYIKVYWFKMLKKIYKALPAKNKKEKMDKFYKKF
                     GAQDYRDSDGVMRQTMVKVINDNLFGLLRLIKAPTLLIWGENDEDTPVYMGRLMEKEI
                     PDSGLVLLKGAGHYSYVDNYDQFRAVINVFLKEDLGE"
     gene            41100..42731
                     /gene="murF"
                     /locus_tag="PKK75_00215"
     CDS             41100..42731
                     /gene="murF"
                     /locus_tag="PKK75_00215"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNZ82046.1"
                     /translation="MQIIVSLYLLFWFYLCGLKIRQSLHIMQLEGYDSDKFLNWIENN
                     KDRFHTRNDKIYTIVLIIISVILAVTSPIEKIIIYPLIYAGISIFFMIKNNREKEESK
                     KPLVFTPRAKRLYGAAYFLLAFDLVLTILIVRWITKDILSYLPVWAGILTVIYYFSSL
                     YIYGANFISKPVEERINKRYYIQASEKIKKMNIYSIGITGSYGKTSTKYISETILREK
                     YKVLKTPESYNTPMGISKIINNELSGEYDVFIAELGASKTGDIDEVAKLTNPKLGIIT
                     SIGPCHLETFKSIDNIMRTKYELIENLPDDGIAIFNYDNEFVKKLADKTFKEKVLYGI
                     DNIEETDVFATGIHVDSLGSSFTLCIKSLGTIECKTKLLGRHNILNILAGAAAASILG
                     LSLDEIKRGISKLEPVEHRLQLIDQGTGVVVIDDAFNSNPDGAKAALDVLNSFTDRRK
                     IIVTPGMIELGSIEESENFKLGEYIAVVCDIAILVGKKRTLPIYQGMKKHNFNEDNLY
                     VVNSLNEATEVLKTITKVNDAVLFENDLPDTYDEK"
     gene            42746..43900
                     /locus_tag="PKK75_00220"
     CDS             42746..43900
                     /locus_tag="PKK75_00220"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNZ82047.1"
                     /translation="MKKIGIIIGGKSVEHEVSIITGMQVFENIDRTIYEPRIIYIQKD
                     GKWLVGESLHDINNFKAKKLEDAYEVLPGFKEGKLILYPHPELKTGIFGKKYSVYEID
                     VVFPAVHGTNVEDGSLQGLFQMNGVPYAFGSVLCSAVGMDKIIMKKVFQSYGLPVVDY
                     TWFYRSSFVENKDEIIKRAENIGYPLIVKPANLGSSVGINKAKNLDELLFAIDVAMSY
                     DRKIILEKCIENVREINCAVLGYEDELETSLCEEPVGWKEFLTYEDKYVNKKKDSRES
                     KKRIPAEIDEEIENQIKYYAMEAFKAVDGCGNARIDFLLDGNNIFVNEINTIPGSISF
                     YLWEGKGISFKQLITRIIQLAEIHGSQRKVNIVSYDIDLLNKMGGGGKKK"
     gene            43915..46026
                     /gene="rnr"
                     /locus_tag="PKK75_00225"
     CDS             43915..46026
                     /gene="rnr"
                     /locus_tag="PKK75_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNZ82048.1"
                     /translation="MNFKDKILAFMKDEAYKPLTFKELLQAFEVEGKMKKALLTALNE
                     LEDEGKIIFTRSERYGLPEKMNLVTGTLEGNQKGYAFLIPDDKNLKDLFISPVDMNSA
                     MHGDRVIVRPMKVLEDVKSPEGKVIRIIERANQYVIGTFQKSRHFGFVVPDDKRIAFD
                     IFVPREEFNNAEENNKVSVKITEWPDQRKNPEGTIVEVIGDSEDTKTHIDAVLLAKKV
                     RQVFPEDVIKEAKRVSEEGIHELELKRRKDLRSLDIITIDGSDAKDLDDAVYAKKLND
                     SEYFLGVHIADVTHYVKEDRKLDKEALKRGTSIYLTDRVIPMLPKELSNGVCSLNPGE
                     DKLTLSVEMIINNEGKVIDYKIFESIMNNRYRMTYEDVTDILENNNEELIEKYKDIVP
                     MLKTMEELSLILRRKRDLRGAIDFEFPERKIITDEEGRAIDVTKYERRTSNKIIEEFM
                     LVCNETVAEHYYWLNMPFVYRVHEDPDEEKMYEFSKFIHNFGYTLKGNDIHPKELQQL
                     LNKIKNTKEESLINTMMLRSLKKAIYSPDSSTHFGLAANYYCHFTSPIRRYPDLQIHR
                     IIKGQINGRYSEQALQKLFERTAVVAEQSSKMERIADEVERDTEKIKIAEFMSDKIGE
                     EYEGVVSGVTSFGIFVELENTVEGLVHISNMVDDYYIYDDEKKELFGQGSRKVFKIGN
                     AVKIRVDKVSIAKAEIDFALI"
     gene            46139..46490
                     /gene="ssrA"
                     /locus_tag="PKK75_00230"
     tmRNA           46139..46490
                     /gene="ssrA"
                     /locus_tag="PKK75_00230"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            46971..47456
                     /locus_tag="PKK75_00235"
     CDS             46971..47456
                     /locus_tag="PKK75_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82049.1"
                     /translation="MIEATEEICSDLQEKLSDHFRFSKKNKGIAFSVPFIRWKLKSNE
                     EEIRELHRNFNPSHFCIMTIIDKGKSNDCIKAAKAAGARGGTVMHGHGAGVPTDYYFP
                     LIIEPQKDVVMVIAGKDNVNAIKDRIINEMELFKEGKGIIFILPVVKISGFSDNQEVK
                     A"
     gene            47453..48901
                     /locus_tag="PKK75_00240"
                     /pseudo
     CDS             47453..48901
                     /locus_tag="PKK75_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006907390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            49407..50279
                     /locus_tag="PKK75_00245"
     CDS             49407..50279
                     /locus_tag="PKK75_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNZ82050.1"
                     /translation="MNNFKKFLYETLYLTTGCFITAFSVNYILKPNGLITSGITGLSI
                     ILERYLLIDYYYIYYMATLIILASTLLLMGKKELMRIIFLSVLYPTVLLLMQGVEFKF
                     VENDLLLASIYFSLFYGIGVGMILRKGFSFGGTDTIARILNKKLFPSINVSYLMLVLD
                     GFVIIAAAFTFGRNIALYSIISQLIFTKVCDYIMFGFGTKLYKLEIISSQFEEISNYI
                     MHELGRGVTLFKIKGCYTNEEKVQIESICSPNEAVMVQRLIKKIEPGAFVKVLPVVNV
                     WGKGRRFIDISNED"
     gene            50297..51262
                     /locus_tag="PKK75_00250"
     CDS             50297..51262
                     /locus_tag="PKK75_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNZ82051.1"
                     /translation="MNNSGDLPEYVTVVGGVNVDIQGFTKHKLIPGDSNIGRVKISLG
                     GVGRNIGENLIRLGINTKLISVVGDDVYSSKILNDSRETGLDISDSMAVKCENASIYL
                     AVLDEDNDLHIAINSMDIMEKMTVDFIKEKKHIFDKTRLLVMDTNIPQDVIEYLLHEY
                     KRKDIFLDTVSVSKAKKIKNLVGHIHTLKTNKLEVQALTEIEVTDEDSLVKNGQYLLN
                     RGVKQIFITLGKNGVFYLNKSTKRIIRADNIKPVNATGAGDAFMSALIYCHLKGIDIN
                     EAAKFATAASIIALSHENTINPNMSKENINIKEKELKYYEKLFEC"
     gene            51237..52154
                     /locus_tag="PKK75_00255"
     CDS             51237..52154
                     /locus_tag="PKK75_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNZ82052.1"
                     /translation="MMKNYLNVNPEVKEALEKGKAVVALESTIISHGMPYPKNVETAL
                     NVEKIIRDNGAVPATIAILGGVLKVGLTEEEIEYLGKAENVLKTSRRDIPFIVSKKLD
                     GATTVASTMIIAAMAGIRVFATGGIGGVHRGATKTFDISADLEELSNTDVAVVCAGAK
                     SILDIGLTMEYLETHGVPVVGYKTVEMPAFYTRKSGFKVDYTVDSPVEIAEALKAKWD
                     LGLKGGLVVANPIEEQYQMDYDTITAAIDKAVSEAEETGIKGKESTPFLLSKVKEITG
                     GDSLESNIMLVYNNALLGAKIAVELEKIS"
     gene            52221..52919
                     /gene="pyrF"
                     /locus_tag="PKK75_00260"
     CDS             52221..52919
                     /gene="pyrF"
                     /locus_tag="PKK75_00260"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNZ82053.1"
                     /translation="MGKDVIVACDFGNKEEVKYFLSKFTEEKPYVKIGMELFYREGPE
                     IIKLISSMGHKIFLDLKLHDIPNTVKKTMRVLSGQNIQMVNLHAAGSIPMMEAALEGL
                     TREDGSRPLLIAVTQLTSIDEETMKRDILIDKPLEEVVIHYARCAKKAGLDGVVCSPL
                     EAERVHTACGIEFLTVTPGIRFEGNEPGDQKRVTTPAMAKISGSDYIVVGRPVTEAKD
                     PVEAYRRCLREFTV"
     gene            52928..53377
                     /gene="smpB"
                     /locus_tag="PKK75_00265"
     CDS             52928..53377
                     /gene="smpB"
                     /locus_tag="PKK75_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNZ82054.1"
                     /translation="MADKIVAKNKQAYHEYFIEEKYEAGLVLAGTEVKSIRQGKVNLK
                     DSYVSIKNGEAFVYNMHISPYEKGNIYNTDPLRIRKLLLNKREIRKLNGMTTLKGYSL
                     IPLSLYLKNGLVKMELALAKGKKLYDKRQDLAKKDADRRVLRQDDFR"
     gene            53404..54099
                     /locus_tag="PKK75_00270"
     CDS             53404..54099
                     /locus_tag="PKK75_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNZ82055.1"
                     /translation="MKTLYEGKTKNLLKNEKDGFVYLLFKDDATGENGVFDPGSNTVG
                     GSVEGKGKLGLIISSYFFELMEKNGIPTHYLGADIDKGLMKVRSLTVPKLEFVLRYFT
                     AGSMCRRFSMEEGIPFDPPYTEVTLKDDEQGDPLISERLCMMKGLLKEGQHDEAMNVL
                     VKVGKVLKEELKQMNLTLIDFKIEIGYDENGKIFVVDEITPDIWRVRDKNGTIPNQIE
                     CGKIVLEKIRNAV"
     gene            54122..54538
                     /locus_tag="PKK75_00275"
     CDS             54122..54538
                     /locus_tag="PKK75_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HNZ82056.1"
                     /translation="MLAELIKNGFGEKEDLNCAEKILWGANNAYNLGLDREALKLASG
                     FGGGMAIEDACGALTGAIMVLGKLFVVERAHESDKIKELTRELFDEYNKEMKSIDCKP
                     LKDMYRTEELKCRNVILKAAEILDMIVLRELNKPAS"
     gene            54746..55366
                     /locus_tag="PKK75_00280"
     CDS             54746..55366
                     /locus_tag="PKK75_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNZ82057.1"
                     /translation="MKKVLTIVLVFALSFGFLTLVNNMGGKNTASAQETLTGKAEGFG
                     GEINVTLIVNGDDIVSVEATGDDETEGIGSLAIDEVPALIQEADSADVEGVSGATYTS
                     DGIKAAVRNALASKGGSSSEQTLTGTAEGFGGDINVTVVVKGNDIISVEATGDDETEG
                     IGSLAIDELPALIQEADSADIEGVSGATYTSDGIKAAVKSALESKQ"
     gene            55522..55950
                     /locus_tag="PKK75_00285"
     CDS             55522..55950
                     /locus_tag="PKK75_00285"
                     /inference="COORDINATES: protein motif:HMM:NF025304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ82058.1"
                     /translation="MDQNNNNIQKRKWKQISEKERYKIEALYEQGLKAKQIGECINPM
                     RDRRTIERELQLGIVEQRRINPSFNKYQPLYITEKVYKADTAQMRHNERASNKGRGLK
                     IGHDQELADYIENKIKDEKWSPDVVIGRIKAENIPFKTTK"
     gene            56367..57620
                     /gene="eam"
                     /locus_tag="PKK75_00290"
     CDS             56367..57620
                     /gene="eam"
                     /locus_tag="PKK75_00290"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular transferase
                     activity, transferring amino groups [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HNZ82059.1"
                     /translation="MKKTSREVSLERAEVLKSRIDPYLEAKKEIPLGLDFQDEIKERQ
                     KKIKEYFNATDEEWNDWHWQISNRIHDAETLNQFIHLTDKQKADIDKVGEKFRWAISP
                     YFLSVINPKGNHFENPIYLQSIPTGLELVEGIGEADPMGEEFTNPAPCITRRYPDRLV
                     INATNVCGMYCRHCQRRRNIGEFDTAQPRENLQAAVDYIAANKEIRDVLVTGGDPFTL
                     SDDTLDWLLTQLDNIPHLEFKRIGTRMPVTLPQRVTKEMCDMLKKHHPLFINLQFNNP
                     LEITEETKKACSMLADAGIPLGNQSVLLRGVNDDKFVMKRLNQGLLSCRVRPYYIFHA
                     KGVVGTLHFRTSVDIGIEIMEHLRGYTSGLANPTFIINAPKGKGKTPMLPEYVISHGR
                     DTITIRTWEGTIIEYPNKDANVKYE"
     gene            57790..58836
                     /locus_tag="PKK75_00295"
     CDS             57790..58836
                     /locus_tag="PKK75_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNZ82060.1"
                     /translation="MKKLLSLMVVCILVLSLAACGEQQTPPPASNDTDKPAEETPETP
                     EVTRDPVDLVMGTGSSGGTYFALGGAMSNAWNSFLKDEQITVTAQASGASVENINLIN
                     AGEMDLGMAMNNVAANAFAGTGAFNAPVTNVSSIGVVYNEVYQIVANAKTGAKNVEDL
                     KGLKMAVGPAGSGTVGLTQKVLAAAGLDIDKDIKRQSDSFGDAATKMQDGHIDAAANV
                     LAVPASSIIEMATVMDLAYVDISDEILATIQADEPYFVRKVIPAGTYKDQAEDINTIT
                     CKAALYCRADLDEETVYQITKAMYETNDVVAAAHTTGKEIQLEGALEGITTPIHAGAA
                     RYYQEMGIEVPDNK"
     gene            58970..61099
                     /locus_tag="PKK75_00300"
     CDS             58970..61099
                     /locus_tag="PKK75_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HNZ82061.1"
                     /translation="MSKDKNEKHIPPVDEDNIDKVNEENIVFNENNKTVDKESIDTDK
                     EEVNYLGDMSGIDADELMAQYDRESAFRRLIGVPGKLVFFIAVSWSVFQLYTGFFGTF
                     PSTLQRAPHLAAALTLVYLLYPAKRGKSVEFTKSIPWYDYVLAAASITVGSYHIIFYD
                     ALLLRAGSFTQLDVVVSVIAILLLLEAARRVAGMVIVTVGIVFLVYALFGQHLPRTSF
                     LFHAPFTFKRVVSTEWLGSEGILGTPIYVSSTFIFLFLVFATFLKKSGVGDWITNIAL
                     GAVGGQVGGPAKAAVVASALEGTVSGSSVANVVGSGSITIPLMKNTGYEANFAGAVEA
                     AASTGGQIMPPIMGAAAFIMTEYVGVPYATVATAAVIPAILYFTGIFLNVHYEAVKTG
                     LRGIPKEQRPNTMKLFKEGWYMMTPVILLFGMLIGGSSAMKAAMYGIIGCIIVWIVNI
                     LKEERKFDVVRFVKMFIDSLDESARSAVTVAITCGTAGIIVGVVTMTGLGLKMADGLI
                     ALAGGSLLLTMVFTMFSSLILGMGVPTTANYIIQATISAPALVALGVPPIAAHIFVFY
                     FGIVADITPPVALAAFAGAGISGGNPMKTGFIATRLGIAAYIVPYMLVYNPILVLVNT
                     EGYSIPIFTLVVIKSAVTAVIGMIGLATGFTRYFKTNCKIWESIVLIIAGLLLVDPGT
                     ATDIAGAVLMFAIYLVQKRRIAKTAIS"
     gene            61701..62069
                     /locus_tag="PKK75_00305"
     CDS             61701..62069
                     /locus_tag="PKK75_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82062.1"
                     /translation="MSLSEIIRAIGFFIAAIAIYLVLLVITMKRNIIKVIKVFENKNA
                     LSAKTAISYTELNIQKQGTIDRAIKKRDNRSHALKFLLDGGVVIINGDERYYLNKKKM
                     LAFKQRLNFIGRMMIPDMDD"
     gene            62305..64497
                     /gene="topA"
                     /locus_tag="PKK75_00310"
     CDS             62305..64497
                     /gene="topA"
                     /locus_tag="PKK75_00310"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNZ82063.1"
                     /translation="MKNLLIVESPAKAKTIRDILGSSYIVKASVGHVRDLPKSKLGID
                     IDNEFQPDYITIRGKGDIIKELKKEAKKADNVFLATDPDREGEAISWHLAKILNLDQE
                     KEIRIVFNEITKDAIRNAARAPRKINIDLVDAQQARRVLDRLVGYKISPLLWKKIKKG
                     LSAGRVQSAALKLVIDKEREIMDFKPEEYWSLELELKKSRKKFTARYVGIFENDKLKK
                     VRIKNQEELNRILESIDKEKISVAKIDKSKSFIKPGPPFTTSSLQQEANRRLNFTSKK
                     TMMVAQQLYEGVKVKGEGNIGLVTYIRTDSLRLSDEAIDNAKNYILSNYGEKYYRYRL
                     YKKAKKNESKVQDAHEAIRPTSLLRSPERLKDSLTSDQYKLYSLIWKRALACQMQDAE
                     YDVTKVLFNNNDNIFESSGKILVFDGFRTVYGYTDEKDEKMLPELEINEVLEILGILP
                     EQHFTQPPVRYTEASLIKDLEEKGIGRPSTYAPTITTITSRDYVAKEKGYLFPTDMGF
                     LVTEMMEKYFPKIVDEKFTANMEDRLDDVAGGNVNWPSIISEFYEGFKKDLFVAENEM
                     KEIEVKEEVSDVKCENCGAFMVVKSGRYGKFLACPNYPGCKNTKPLNGREPAEETDEV
                     CEKCGSKMLKRKGKFGEFLACSNYPKCKNTKPINNTIDALCPRCGNKIAVKYSKAGRK
                     FFGCSNYPECNYISWYEPTRKKCPECGDLMVLKDNKDVCVNKKCKISE"
     gene            64684..65457
                     /gene="rpsB"
                     /locus_tag="PKK75_00315"
     CDS             64684..65457
                     /gene="rpsB"
                     /locus_tag="PKK75_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNZ82064.1"
                     /translation="MAIISMKKLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KTSDKVDEAYSFIKEVVSEGDEILFVGTKKQAQESTKTEAARCGMHFVSQRWLGGMLT
                     NYSTIRKRIERLNELKTMEEDGTFKLLTKKEVFKLKNEAERLEKFLGGIKNMDKLPGA
                     LFVVDPRKEKIAVQEAKILGIPVVAIVDTNCDPDEVDYVIPGNDDAIRAVKLLTATVA
                     DAVLEGKQGVQMTYDDVEEDDEVSDEELEKVEEEEFELE"
     gene            65529..66443
                     /gene="tsf"
                     /locus_tag="PKK75_00320"
     CDS             65529..66443
                     /gene="tsf"
                     /locus_tag="PKK75_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNZ82065.1"
                     /translation="MEITASMVKELRDKTNAGMMDCKKALKETSGDIEKAIIYLREKG
                     LLQAAKKSGRIASEGLTTAIISADGEKGVVLEVNSETDFVAKNEEFRNFVTAAAQAAL
                     DNEPADLEALKALVLEDGKTVQDVLTDKIAKIGENMSLRRFGRAKVQKGLVVDYIHAG
                     GKISVLVALESEGDKETLKVLGKDLAMQVAAMSPKYISMEDVDDEFIRQEKEILTAQA
                     LNEGKPQNIVEKMVEGRLNKELKEICLLEQAFVKDADLTVKKVISETAKKIGADIKVA
                     DVLRYEVGEGIEKKSENFADEVAKQMGL"
     gene            66507..67217
                     /gene="pyrH"
                     /locus_tag="PKK75_00325"
     CDS             66507..67217
                     /gene="pyrH"
                     /locus_tag="PKK75_00325"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNZ82066.1"
                     /translation="MCKYKRIVLKISGEALSGSTGHGINSEIVNRISGVIKRINQMGV
                     EVAIVVGGGNFWRGAGARDMDRTTSDYMGMLGTIINGLALQDALEKIDVETRLQTAIE
                     MRQIAEPYIRRRAVRHLEKGRVVIFSGGSGNPYFSTDTTAALRAAEINAEIILLAKNG
                     VDGVYSDDPKKNPNSTKFEELKYIDVLNKSLKIMDSTATSLCMDNKIPILVFGIENPE
                     NIEKIVSGENIGTIIKEG"
     gene            67220..67777
                     /gene="frr"
                     /locus_tag="PKK75_00330"
     CDS             67220..67777
                     /gene="frr"
                     /locus_tag="PKK75_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNZ82067.1"
                     /translation="MYMDLIFKSEELMRKSISFLKEELATIRAGKANPKLVDKIQVSY
                     YGTMTPLNQIANISVPEPRMLLIQPWDAHALKDIEKALQTSDLGINPANDGKLIRLVI
                     PVLTEERRRDLLKVVKKEIENSKIAVRNIRRDTNDEIKKLEKQSEISKDDLKKAEEEM
                     QKLTDRYIDIIDEIYKDKEIEILEV"
     gene            67779..67961
                     /locus_tag="PKK75_00335"
     CDS             67779..67961
                     /locus_tag="PKK75_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82068.1"
                     /translation="MDSLTGYPLKEALGELESSNLIVNIVKVTGRNNRFNNLEKPYVI
                     RDIYSDGTVTLYISYF"
     gene            67983..68702
                     /locus_tag="PKK75_00340"
     CDS             67983..68702
                     /locus_tag="PKK75_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNZ82069.1"
                     /translation="MLEEKVLSGEIPEHIAVIMDGNRRWAKQKNLPAKMGHREGALRV
                     IDLVRTCGDIGVKFLSVYAFSTENWNRDKKEIDYLMNLLVEFVFNELNTLHKNDVKIT
                     MMGNIEDLPDKTRIEVERAVELTKNNKTLHLNIALSYGSRNEILRAVKNIVKECGNKE
                     LGIDEINEESFKRYLFTYNMPDPDLLIRTSGEIRLSNFLLYQMAYTEFYFTEVLWPDF
                     NKNELFKAIESYQKRKRRYGN"
     gene            68713..69480
                     /locus_tag="PKK75_00345"
     CDS             68713..69480
                     /locus_tag="PKK75_00345"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNZ82070.1"
                     /translation="MGQRVLTGFIGGVFIAFVTYLGGSLYDFVFLAITCIAIYELSKI
                     FFGNGFDYGAIVNYFFAMALFIEKITENRHFFDFFLFLYISVNFLIFVFNRKVTIKKV
                     SEILFLGTYVVFFMYHMMLMNSSPFVWLVYIIAFGSDTFAYFTGKFFGKNKLYPEVSP
                     NKTIEGAIGGIAGSTILSLIFFNYLSINKYFYIIIFSVSASVFSMAGDLTASKIKREY
                     EIKDFGNLLPGHGGIMDRFDSVLFVAPAVYYFILHFL"
     gene            69495..70736
                     /gene="rseP"
                     /locus_tag="PKK75_00350"
     CDS             69495..70736
                     /gene="rseP"
                     /locus_tag="PKK75_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNZ82071.1"
                     /translation="MVTLIASILVFSAIVLVHEYGHYKAAKSVGIKVYEFAIGMGPTL
                     IKREKNGTLFSIRAVPIGGFVLMEGEEEDSRTPTSFGTKSIGQRAKVIAAGPVMNFVL
                     ALVLFLIIAFGYGVYGNHVDFIDENSNEYMEGLRTGDKIISINGDRTYIWEDIFYELI
                     SPEENSYTIEYERDGKISKANIDKSYRKIIGITSTLVNGQTTTTVKPSNITSPAYKAG
                     IMEGDVVIKINDIYVNTWDELIGQVENSNKTVKVTVDRKGEILDYNIDLQEQISVRFN
                     TQLEKSFITAITSSMYKTVYYIELMFKTIRQLVTGRLGSDALGGPVMVISMVGEAAQS
                     GILSLLSLAAFISINLGFMNLLPVPALDGSKLIFLAVEGIRGKALPMEKEGYIHFIGF
                     VFLIGFMLFITYKDILRIFRI"
     gene            70758..71822
                     /gene="ispG"
                     /locus_tag="PKK75_00355"
                     /gene_synonym="gcpE"
     CDS             70758..71822
                     /gene="ispG"
                     /locus_tag="PKK75_00355"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNZ82072.1"
                     /translation="MDKIIRRKSTGVMAGNVQIGDGADITVQSMTNTFTKDVKATVEQ
                     ILRLEEAGCEIIRVAVADYEDAEAIKEIKKQIHIPLVADIHFDYRLAIKSIENGIDKL
                     RINPGNIGGRDRVKMVVEHAKPRNIPIRIGVNAGSIHRDILNKHNGFPTSQAMVESAL
                     EHVRILEDLDYNNIVISMKGTDIKTTVNAYKIMAEKVSYPLHIGITHAGTLFEGSIRS
                     AIGVGSLLTEGIGDTVRISLTDDPGEEVKVAKEILKSLNLRNFGINYITCPTCGRTKI
                     SLIELTKKIQEGLKGVNKPISVAIMGCAVNGPGEARQADIGIAGGTGEALLFKKGEII
                     GKVKEEDIVKTLLEEIDKIP"
     gene            71942..72574
                     /locus_tag="PKK75_00360"
     CDS             71942..72574
                     /locus_tag="PKK75_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNZ82073.1"
                     /translation="MAVGALNVEKRLKESSKTNKQLRREGFLPGSISSKGKDSVSVTI
                     KADELRRGLSTYGRNSLFRIILEGREITGMVKDIQLSPVKGTMLHVDFQEVSLTEEIK
                     VVLQIVLRGTDALEFKGLMALRQSDSVTVKGLPQNIPDDLVIDVSNIDRVENIHLKDV
                     AFPEGIIPEGDPEHLLISIAEAKRIAVEREDEEASEDVEAAAADQENKEE"
     gene            72702..73544
                     /locus_tag="PKK75_00365"
     CDS             72702..73544
                     /locus_tag="PKK75_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNZ82074.1"
                     /translation="MKWKGRRGSSNVQDRRGGGTSRGVVGGGIGTIILFVVVLLMGGD
                     PSQLLGNSAFINPAPSQYVESEEENELAEFASVVLAETETVWDDLFEKEGLEYQYPTL
                     VLYTDYVQSACGNATSQTGPFYCPGDNSVYIDLSFYEELKKNYKAPGDFAFAYVIAHE
                     VGHHVQKQLGITDQVMGLRNRLSEKDFNQYMVRLELQADYFAGVWAHYVERANLLEKG
                     DINEALNAASAVGDDRIQSEAWGYVVPDKFTHGTSEQRARWFYKGFESGTMQDGDTFN
                     AKDL"
     gene            complement(73602..74273)
                     /locus_tag="PKK75_00370"
     CDS             complement(73602..74273)
                     /locus_tag="PKK75_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNZ82075.1"
                     /translation="MLYELNIYSVTMRIILAMICGGIIGLERSRKNRPAGFRTYMIVC
                     VSSALVMMTNQYIYNNFTGSDPARLGAQVISGIGFLGAGTIMVTSRSQIKGLTSAAGL
                     WASACMGLAIGVGFYSGAVMVALVVFVVITFFKKIDYIMTSTNRIINVYTSFGSVEQL
                     DKFIVFCKQTGFRIQDIEITKDAVQHEVSVIAIFTLEANKRISHIDIIQKLSSFEGLI
                     HIEEL"
     gene            complement(74485..75591)
                     /locus_tag="PKK75_00375"
     CDS             complement(74485..75591)
                     /locus_tag="PKK75_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNZ82076.1"
                     /translation="MDWRKELLNNLTTAEEVIKALNAKLTDEETAKLTQIIERYPMSV
                     PRYYLSLIDKDDPDDPVRKLCIPSISETDMSGSFDTSGEADNTVVVGLQHKYKETGML
                     LSTSNCAMYCRHCFRKRLVGQNDDEVNKHFEDIISYIKDHIDMTNVLISGGDAFLNSN
                     QKIENYLKELSKIEHLVYIRFGTRIPVVFPMRIYSDPELLDILERYSKIKQIYVVTHF
                     NHPNEITEESKRAVKELINRGIIIRNQTVLLKGVNDNSKTLEKLMKDLVGTGVVPYYV
                     FQCRPVSGVKNQFQVPLKRGCRLIDEAKNNLSGMAKSFRYSMSHKTGKIEILGSMNEG
                     KMIFKYHQTKDPENSGRIFELEVADSQAWLPEEF"
     gene            75931..80178
                     /locus_tag="PKK75_00380"
     CDS             75931..80178
                     /locus_tag="PKK75_00380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HNZ82077.1"
                     /translation="MFLKDAFKDKGLVNIEVEKIEVSENNETVKVHILSREIIGLNII
                     ENIESQLKQHYDNAVDFKLHIEYNLDKSAKKEIEIIKDNIFHIIQKSSSILAKAKDNI
                     NIEFKDDCFFITANNKLLINELDDSKTLGKIKKLMDEYKIDCQAKTIYQEAEDEAAIT
                     AQRIFDEELKKNLELINNNSSSEKKPVNNSQKDNFRKSSRKKFDFDSLPVSLISDITI
                     DSGLVNIKGKIVNVEKRELKKGSFLLLVSLTDSTDTIMCKYFCPKNFDDTGIEKGKYL
                     QAGGNVEFDEYEKEEIVKVQDLRLISEEIKRMDNSKEKRVELHLHTGMSSMDGINNFK
                     EYADKAKLWGHRAMAITDHGVVQGFPDAMKCADDNFKVIFGMEGYLIDDTVNIVHNCE
                     DIRLDSDFVVFDLETTGLNPKDDHIIEIGAVKISDRKFVDSFSTLVHTDKMLSEKIIE
                     LTSITDDMLEGQPDINEALGEFLKFAKGSVLVAHNAKFDTGFLREKSKAAGIENYDPP
                     TLDTLELSKALIKDIKNHKLSTLTKKLGINLINHHRAVDDANAAGELFIILLNKLKDR
                     EIFEISKLNELLKEDIDIVRQPSYHINILVKNKKGLKNLYRLVSDSHINNFYKKPRLL
                     KSNLNRFREGLMLGTACETGELYRALLEGYSDEKIEEIVDYYDFLEIQPLQNNMFLVN
                     SGRLKSEEELKEINRKIISFGDKRGKLTAATGDVHFLETEDEIFRKILMSGQGYEDAE
                     EQAPLYFKTTGEMLDDFSYLGEEKAYEVVVKNTNLIADMIKDVLPIPNGTFPPSMEGS
                     DRDLRKICFDNARSIYGESLPEIVESRLNRELNSIIGNGYSVMYMIAQKLVKKSNEDG
                     YLVGSRGSVGSSFAATMAGITEVNPLVPHYACPGCKYTEFIEDGSYGSGFDLPEKSCP
                     VCGTSLVKDGHDIPFEVFLGFEGDKEPDIDLNFAGEEQAVCMKYTEELFGEGKVFRAG
                     TISTIADRTAFGFVKNYFEERGINKRKAEIDRLIQGCLGVKRTSGQHPGGVMIVPQDK
                     DIYDFTPVQYPANNEKSGTVTTHFDYQSISGRILKLDLLGHDTPSIIRMLEDLTGVGK
                     ETIPVDDPDTMTIFNSPEIFGITLDEINCSTGTLGIPEFGTGFVRQMIIDTKPRSFSD
                     LIRISGLSHGTDVWVNNAQDIIKQGYAEIGGVISTRDDIMTYLIQMGVEKKTAFDIME
                     RVRKGKGLREEDEKAMKAQNIPRWYIDSCNTIKYMFPKAHAAAYVTMSVKIAYFKVHY
                     PEAFYAAYFTTKVDDFDADIVTRGQNYILSTIKYLEEKGNDKTAKEKNLITVLEVAYE
                     MYKRGFKFTKVDLYKSDDRKFLLAKDGILPPLMGLPGLGANAAANIKAEREISEFISK
                     EDIIKRAKVSKTVIEVMENHGCLKNMDETNQISIFNV"
     gene            80489..80953
                     /gene="rimP"
                     /locus_tag="PKK75_00385"
     CDS             80489..80953
                     /gene="rimP"
                     /locus_tag="PKK75_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNZ82078.1"
                     /translation="MTKMNITSSVRKMTEEVLKTTEMELVDVEYVKEGPFRYLKIYID
                     KPGGITVDDTADISRILNKKLDEADLIDEQYFLEVSSPGVERPFKKEKDYLNNIGNFV
                     EARFYKPVEGKKSVKGLLKEKNENDIVIETGEGILRIELSDLSKINRVLEDF"
     gene            80966..82024
                     /gene="nusA"
                     /locus_tag="PKK75_00390"
     CDS             80966..82024
                     /gene="nusA"
                     /locus_tag="PKK75_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNZ82079.1"
                     /translation="MNKEILNALVELEREKGINQDVVLEAIRSALETGYKKNYGKATN
                     FSIEIDKVNGEYRLYQDKTVVAGVENSNLEISLEEAKTIDKRYELGDIVKIEIKPKKD
                     FGRIAAQTARQVILQKIREAERESIFSEFYGRESDILTGVVQRENKGNIFIDLGKIEG
                     ILAVNEQIPGETYNPGDRIKTVILEVKNTGKGASIMLSRSHPNLVKRLFELEVPEIHD
                     GTIEIYSIAREAGSRTKIAVFSHDPNVEPIGSCVGNKGTRVKNIIDEINQEKIDIIIY
                     EKDPEKFIANSLGPAKIIKVTANEKEKSTVAIVPDNQLSLAIGKEGQNVRLAAKLTGW
                     KIDILSESQYSQRLQEEV"
     gene            82021..82290
                     /locus_tag="PKK75_00395"
     CDS             82021..82290
                     /locus_tag="PKK75_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNZ82080.1"
                     /translation="MKARKIPMRKCIGCMESFPKKELCRIVKNKENEIFFDSTGKLNG
                     RGTYICKNIECFNKAIKTKRLSKSLETEIPDEVYEAIKAVIEKNA"
     gene            82283..82576
                     /locus_tag="PKK75_00400"
     CDS             82283..82576
                     /locus_tag="PKK75_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNZ82081.1"
                     /translation="MHKIYSILGIARKGGNVSIGFDAAKADIEKGKSFLVVIAEDASE
                     KTKKNIIFTCNKHDCKYMEFGKKEELGKNLGKEVVSVMSIRDKNLASYVTNNI"
     gene            82592..84778
                     /gene="infB"
                     /locus_tag="PKK75_00405"
     CDS             82592..84778
                     /gene="infB"
                     /locus_tag="PKK75_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNZ82082.1"
                     /translation="MRIYELAKELDMNSKDLCKLVEKELGITVKNHMSSLTDLEVLRF
                     KKALSLMNSTKKTVKGPKNVVIDDEPLVDEKIDLNQAKLKKVDKKLLTPVKKPLPSKS
                     KKVKTPAETGKTDYRKDAAKDKQKQQKKPQQSRPEPKQHKVRTISIEDSIVVRDFADK
                     LGVPVNVLISKLILLGVMAGMNQEIDYDTAALIGEELGAKISKIEVVDELQDLEELLS
                     KDDEDDEKEMVPRPPIVTVMGHVDHGKTSLLDAIRDTSVTKQEAGGITQHIGASQVEI
                     NGKKITFLDTPGHEAFTAMRARGAKVTDIAIIVVAADDGVMPQTVEAINHSKAAGVPI
                     IVAVNKIDKPTANPDKVKQELSEHGVISEEWGGDTIFVSVSAKKRMGIEELLEMILLV
                     AEVQELKANPNRSAKGTIIEAKLDKGRGPVATVLIDKGTLKKGDYVLVGSTHGKIRAM
                     FNSKGKRINQAGPSVPVEIVGLSETPQAGDMLTVMENEKDAKNIAEKRKDKKHLESIN
                     VSAKVSLDDLYERIQKGEVKDLNIIIKADVSGSIEAVKQSLEKLSMAEVKVKPIHGGV
                     GGINENDILLASASNAIVIGFNVRPSVAALDLAKQENVDVRTYNIIYNAIEDIEAAVK
                     GMLKPVYREVVMGRADVRQIFKVPNVGLVAGVYVTSGKITRKSKIRLLRNDIVIHDGQ
                     IASLKRFKDDVSELASGYEGGIGIEKYNDIKEGDSMEAYVMEEIKR"
     gene            84799..85176
                     /gene="rbfA"
                     /locus_tag="PKK75_00410"
     CDS             84799..85176
                     /gene="rbfA"
                     /locus_tag="PKK75_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNZ82083.1"
                     /translation="MAKRRNNRLSGEVKRAISEIIRNDVKDPRISELLSITEVHVTED
                     LKYAKIYVSDYNDIETSMIALESAKGFIRKEIGKKVKMRIIPELIFIRDDSIEKGMHM
                     SSLIGKIIEEDNVRKVNEDNAEE"
     gene            85139..86128
                     /locus_tag="PKK75_00415"
     CDS             85139..86128
                     /locus_tag="PKK75_00415"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNZ82084.1"
                     /translation="MSERLMKIMRKNDVKEVFDLINNSDRICIAGHKAPDGDCIGSVM
                     ALYSFLKPLGKQLTVMMDGIIPFNYKSFVDESIILKNYGNEEFDLLFVLDTSDRKRLG
                     KFEGVLSNSKKTVVIDHHITNEGFGDINIIEPHMSSTGELLYGLLKLADMEISSETAV
                     LIYIAVITDTGKFSYQNTTAKTHIIAAELIDLGVNVAEIDNIVYNSKPANIVRAFIDC
                     ISTMELYYGNKLGITAITKEILDKNKAEMADVDGVVEYIREIKEVEVSCLLKENAEGT
                     KVSLRSKNDIDVAAVSLKYKGGGHAKAAGFDIGENMENTKMIILKEFKEYFDK"
     gene            86125..86991
                     /gene="truB"
                     /locus_tag="PKK75_00420"
     CDS             86125..86991
                     /gene="truB"
                     /locus_tag="PKK75_00420"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNZ82085.1"
                     /translation="MTGILNILKPTGMTSHDVVSAVRKILNIKKAGHAGTLDPNAAGV
                     LVVCTGKATKFSEYLMSGQKEYVCELILGKSTDTQDSYGIVINESDKDVVLDELNEAL
                     KKFTGEQIQIPPAYSAVKLNGRKLYEYARQNISVEKPGRTIRIEDIKLLKFKDRSALL
                     KVRCSKGTYIRTLCSDIGKSLGTYGHMGILIRTESKGLHIKNSVTLDRLKFLSDSKRL
                     NECLIPVEDIYKLDKLNIDSKYYDRLVSGNEVDLGISIKNDEFYVYCRDSLIGTGKKT
                     DKTKVKIDKMLI"
     gene            87003..87755
                     /locus_tag="PKK75_00425"
     CDS             87003..87755
                     /locus_tag="PKK75_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HNZ82086.1"
                     /translation="MNDKNGSCIAIGNFDGLHTGHDVLIRRMIELSKETDRESIIITF
                     KYIRNDLKKSSMNIKYINSPEMKLAILKSYGVDRVVEIELNEEVSKYSPEKFIKLLKD
                     DYNAKNIVVGYNFTFGHKAMGNINTLIEFQDKYGYRVEEIPPVRYNGIAVSSTLIRTL
                     LNEGKIYEANTLLLNKYTIYYDEINIDYNKNMGFVDNKSSILVPPDGKYIVSMGRKEY
                     ILSIVSSKAGSSLIFDKKVEEDRDIVFITKAS"
     gene            87888..88151
                     /gene="rpsO"
                     /locus_tag="PKK75_00430"
     CDS             87888..88151
                     /gene="rpsO"
                     /locus_tag="PKK75_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNZ82087.1"
                     /translation="MNKEQKTQIIEQYRINEKDTGSPEVQIALLTFRINNLTEHLKDN
                     KKDHHSRRGLLQMVGQRRGFLNYLKETDIERYRSIIAKLGLRR"
     gene            88203..>89131
                     /locus_tag="PKK75_00435"
     CDS             88203..>89131
                     /locus_tag="PKK75_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNZ82088.1"
                     /translation="MEKIFKYILAGRELKVTVGKIAELTNGSCIVQYGDTVIMANVAA
                     SKEPREGIDFFPLSVDFEEKLYSVGKIPGGFIKREGRPGEKAILTSRLIDRPLRPLFP
                     KGYRNDVSVTVTVLSVDQDCSPEIAGMIGSSIALSISDIPFEGPTGSVNIGLIDGNFI
                     INPNSSQREATSLDLTVSGTKEAIMMVEAGADEVKEEVMLDAILFAHEEIKKICDFIS
                     EITKEVGKEKSCYTNFVCDETIEKEIREYAAPIINQAIIEEDKLKRQENIDSFNAKAK
                     EIFLEKYPENGQDIDDALYSIIKEKVRENILVRG"
     assembly_gap    89132..89231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <89232..90143
                     /locus_tag="PKK75_00440"
     CDS             <89232..90143
                     /locus_tag="PKK75_00440"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNZ82089.1"
                     /translation="PGRREIGHGALAERALVPVLPNEEDFPYTIRLVSEVLSSNGSTS
                     QASVCGSTLSLMDAGVPIKSPVAGVAMGLMKEGDNVVVLTDIQGMEDFLGDMDFKVAG
                     TKDGITAIQMDIKVHGIDKEVLTNALEKAHKGRMFILDKMLECIDKPREEVSKYAPKI
                     ITMMIDPDKIRDVIGSGGKVINKIIEETGVKIDIEDDGSVSIAAENVESAYKAKRIIE
                     SIVKEIEVGEIILGKVVRIESYGAFIKLSDNTDGLLHISQIDSKRIEKVEDVLKMGDE
                     VLVKVIGIDEKGKIKLSRKAAMQQKEN"
     gene            90308..90511
                     /locus_tag="PKK75_00445"
     CDS             90308..90511
                     /locus_tag="PKK75_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82090.1"
                     /translation="MKFFCNSGFIRKAIIVTLLSIIILLLLMGRFRTMRIRILGLAEG
                     GYICRTAETYLQSHGDAPMLLWG"
     gene            90576..91856
                     /locus_tag="PKK75_00450"
     CDS             90576..91856
                     /locus_tag="PKK75_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ82091.1"
                     /translation="MIKKHTLDNGIRIVMEKIDYVRSASIGIWVNVGSNNENDETNGL
                     SHFIEHMLFKGTKNRKANEIAEEIDSIGGQLNAFTSKECTCFYIKVLDENIKEAVDIL
                     SDMFFNSVFSQDEIDKEISVVIEEIKMYEDSPEDIVHDKLSEIIFNESPMAYNILGTE
                     KNLKTFTHDKVKSFMEENYSPSNTVISIAGNFDEEKFIELIETEFKSWENKKVRKVHK
                     ESNYQRKVAGINKDLEQLHMCIGNKTFGRHDENYHSLLVLNNLFGGTMSSRIFQEVRE
                     KKGLVYSIYSFVSNYSDTGIFTIYAGMSYEQAEDALRTILKEMVSIKNGNIKEEEFLR
                     AKQQIKGNYILSLESTSSRMSAIGRRELLYNEILYPDEVIEAINNVKIEDVLDVAKDL
                     FNIDNLSITFTGNLKKHPDLEGKINKVLGEKYDN"
     gene            91846..92280
                     /gene="dut"
                     /locus_tag="PKK75_00455"
     CDS             91846..92280
                     /gene="dut"
                     /locus_tag="PKK75_00455"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNZ82092.1"
                     /translation="MIIKIVKKNPFKLPCYETKGSAGVDIQAFVEAPMVLKPMERALV
                     PTGLFIELPEGYEAQIRARSGLAIKHGISLVNGIGTIDSDYRGEIKVILINLGSEDFT
                     INSGDRIAQMVVMKHEQAKFELVEDLNETERGQGGFGHTGIH"
     gene            92302..92535
                     /locus_tag="PKK75_00460"
     CDS             92302..92535
                     /locus_tag="PKK75_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HNZ82093.1"
                     /translation="MKLSELYSKEIINVDTGENLGIFGDCDLVIDEKTGEIISFISGN
                     NSHFAFFKEPKRKEILWKNIKKIGSDMIIIENE"
     gene            92795..93598
                     /locus_tag="PKK75_00465"
     CDS             92795..93598
                     /locus_tag="PKK75_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNZ82094.1"
                     /translation="MENKSTNSQQQPQTSFNNLDGQRNNKQNQFRDEPVNPFIFSGTP
                     SENEDSSEPIENVKQVGSITEVEPVKDVCFLSIIGEIEGHTSLPPQSKTTKYEHIIPL
                     IIGAEMDPQIKGVLLLINTMGGDVEAGLAISELVASITKPTVSLVLGGGHSIGTALAV
                     SARYSFIAPTGTMTIHPIRTSGLVIGVPQTFRYFQKMQERITNFIVMNSHVDRQALLD
                     YMYDTDEIANDVGTVLNAEEVVNIGLIEEIGGFSSAMKKLRELIDDMGK"
     gene            93752..96097
                     /locus_tag="PKK75_00470"
     CDS             93752..96097
                     /locus_tag="PKK75_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNZ82095.1"
                     /translation="MAKTSANKTTRTNKKTNTNKKGTSTRASGKDILIKEITGIFLVS
                     FAVFMIIVTTGESQTGIIGRSINSFTNSAFGAGSEVLPYFLLAIGVTRLLNIVILNDN
                     NQIAAIIGFFICFIIYRAVSDPEVYSQIALTKDLQELFIISKDLGMAGKGGGISGNLI
                     SSIFVKLIGKNGTYIALGAFIFSLIVLLTNQSIIGILKLISNYIKKFFNVIYKFIFTE
                     DVENKRKKEKLPKQQPNENIKIYDYMSPAQDNKKAEITQLKIEDIQKAENKTDNRTET
                     QKDEPLPDAKEKFEKKVITDDIITISDVKADNYKLPSIRHLNPMPLNVRNDKEDLKKD
                     GEKLIDTLNSFNIPCNILQINKGPTITRYEIQPAPGIKVSRITALSNDIALALASSEI
                     RMEAPIPGKAAIGIEVPNRAKTSVYLRNMIESREYRIAPTNLPFALGKDIAGKNIIAS
                     IDKMPHLLIAGATGSGKSVCINSLIMSILYKAKPDEVKLILIDPKVVELSNYNGIPHL
                     LIPVVTDPRKAANALNWAVSEMTNRYKVFAQNSVREIDSYNEKMAVENKEKMPKIVII
                     IDELADLMTVASAEVEEYITRIAQLARACGMHLVIATQRPSVDVITGVIKANIPSRIA
                     FAVSSHIDSRTILDTGGAEKLLGKGDMLYHPIGLPKPVRIQGTFVSDDEIKRVVDFIK
                     EQQVEIKHNKQNEIIKEIESQTGDDDDVDEYLEQAIETVVNDGQASVSYIQRKFRVGY
                     SRAARIIDQLEERGIVGGHEGSKPRKVLVTKEELDEMRGNS"
     gene            96101..97447
                     /gene="rimO"
                     /locus_tag="PKK75_00475"
     CDS             96101..97447
                     /gene="rimO"
                     /locus_tag="PKK75_00475"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNZ82096.1"
                     /translation="MKKIYMHSLGCSKNLVDSENMLGILTEKGYKTTDYADKADYIII
                     NTCSFINDAQEESVNAILSAADIKNTLNKKTKIIVTGCLSERFGAELGKEIPEVDIIV
                     GTGGFEKIDQYIQEFEKNKKLVMDTGSELDIDKDLPRNILTEPWYAYLKIAEGCSNNC
                     TYCVIPKLRGPFKSRIIENIVEEAKVLSERGAKEIIIIAQDTSKYGLDIYGERKLHEL
                     VGRISEIKGIKWIRVHYLYPEDIYDELIEEFKNNKKLLKYFDIPMQHINDRILKRMNR
                     NTNREQITSLIDKIRREVEGAVIRTSLITGFPGETWEDHEELKNFLKEYRLDRVGIFK
                     YSREEHTPAYRLPEQIDEDIKEERLKELMEIQQAISYENNLLKIDRTYDVLIEEKEDE
                     HIYAGRTYMDSIEIDGCVYVTSDEELTLGNIYKVKINDALEYDLIGGIEDENNKLV"
     gene            97425..97988
                     /gene="pgsA"
                     /locus_tag="PKK75_00480"
     CDS             97425..97988
                     /gene="pgsA"
                     /locus_tag="PKK75_00480"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNZ82097.1"
                     /translation="MKIINWFKDLNLPNKLTVIRVLAIPFFLLFLYISGGIFRFLPLL
                     IFVGAAVTDAFDGHIARRDKLVTDFGKFTDPLADKLLTASAFIAFVELGYISAWIVVL
                     IISREFIVSGFRTLAASKGVKIAANAWGKIKTVFQMVLIIVILLNYTGYFGFTDFLIT
                     PLIAITVLLTVTSGAVYIYENLGVIKN"
     gene            98056..99087
                     /gene="recA"
                     /locus_tag="PKK75_00485"
     CDS             98056..99087
                     /gene="recA"
                     /locus_tag="PKK75_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNZ82098.1"
                     /translation="MEKDKALELALAQIEKQFGKGSIMKLGENAKLNIEAIPTGALPL
                     DIATGIGGVPKGRIIEIFGPESSGKTTVALHIIAESQKRGGIAAFIDAEHALDPAYAK
                     NLGIKVEELIISQPDTGEQALEIAEALVRSGAIDVIVVDSVAALVPKAEIDGEMGDSH
                     MGLQARLMSQALRKLAGAISKSNTVAIFINQLREKIGIMFGNPETTTGGRALKFYATM
                     RLDVRRTETLKKGDDVYGNRTRVKVVKNKVAPPFKQAEFDIIYGKGISKEGCILDMAV
                     EAGVVNKSGAWYSYENNRIGQGRENSKDYLLSNPELMAEIEKKVLEKYEIGALKLSSD
                     DREDTSVDD"
     gene            99435..100985
                     /gene="rny"
                     /locus_tag="PKK75_00490"
     CDS             99435..100985
                     /gene="rny"
                     /locus_tag="PKK75_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNZ82099.1"
                     /translation="MGGAELFEEILVGIICAIIGIIIGFFIRKNISEGKIGSAEIEAK
                     RIIEDANQKAETRKKEILVEAKDEAHKLRNEYERENKERRNEIQRNEKRLIKKEEMLD
                     SKSLVVEKKDEMLQKKLKEVETKQEKLEAIHQKQIEELEKISGLSTEEAKDILINNIK
                     REAEQEAAVAVKEIERESKETAERKAREIITYAIQKCSADHVAETTVSVVDLPNDEMK
                     GRIIGREGRNIRALEQLTGIDIIIDDTPEAVVISGFDPIRREVARLALEKLIADGRIH
                     PARIEEMVDKAKKEVDQIIKDEGEQATLDTGIHGLHPELVKLLGRLKFRTSYGQNVLK
                     HSIEVSYLAGIMAAELGADVKVAKRAGLLHDIGKAVDHEVEGPHVQIGEDLAKKYKES
                     KAVIHGIAAHHGDIEPQTVEAVLIQAADAISAARPGARRETLETYIKRLEKLEEISNS
                     FEGVEKSFAIQAGREVRILVKPEATSDSSIILIAKDIVKKIEEEMEYPGQIKVNVIRE
                     IRATEYAK"
     gene            101183..102787
                     /locus_tag="PKK75_00495"
     CDS             101183..102787
                     /locus_tag="PKK75_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014459186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HNZ82100.1"
                     /translation="MNYNPAEYKYPSRRNVIYGNNGMVATSQPLAAQAGLEILKKGGN
                     AVDAAIATAACLTVVEPTSNGLGSDAFAIVWFNNRLQGLNASGYSPYSISIEELRERG
                     YTEIPKYGVIPVMVPGAPAAWVELNDKFGALPLIEVLKPAINYARNGFPVSVTVKKAW
                     DAASEIYKEENKEELKYWFDTFTKDGRTPRFGELVKLPFHAGTLESIGRTNGSSYYKG
                     HIADKIDAFFKKYKGYLTKEDMENYKPEWVNPISVNYRGYDVFEIPPNGQGLVALMAL
                     GILKNFDFTFKENSDTYHRQIEAIKLGFADGQKYITDSRHMKIDIKQLLSDEYGRIKS
                     NSIKEKALMPKEIDSNRSGTVYLCTADKDGNMVSYIQSNYMGFGSGVVIPETGIALQN
                     RLHNFNFDNDHSNCLMPHKKTYHTIIPGFLMKDGKALGPFGVMGGFMQPQGHVQVVTN
                     MIDFHMNPQEAIDAPRWQWINGINVEVEKDVPVNIVRDLENRGHKVTIQPNEALFGRG
                     QMILRTNEGTLCGGTEKRADGHIAVY"
BASE COUNT        36644 a        16106 c        22190 g        27629 t
ORIGIN      
        1 tattggtaag accgccgaga attaaacagg caataacact gagcgatttt atgttttcag
       61 agcccctggg tctggaaatg atttacggca ttttaaaaga tgaccatgaa gttgaaatct
      121 ttgacatgat tatagaagct gaaagcttaa ccaataaagt taatgaatac atgccggaat
      181 tcgtgggcat tacttctctt tgcattgatg ttataaaggt attggaccta tgcagggaga
      241 ccaaaattgc caataaaagc ataataacgg cagtgggagg gactcaagca tatctgggac
      301 ctgaaagctt ttataatgaa aatgtggatt atattttcca atatacaacg aaggataaca
      361 ttaaaaaatt ctttgcttca cttaacgggc agggaccggt tccaatagac ggcataagtt
      421 caagagtatc ctcatacaag tccacaggac aaaagggaag aaatgaatat atgcttcccg
      481 atagagactc aacaaaaaaa tacaggaatg aatattccta ttttggctat aaaccggctg
      541 caataatgga atttggcaca ggctgtgaaa aggcatgcag cttttgttta aggtggagga
      601 tcgaaggagc ggaagaaaag cttatagata ttgagcttaa caaaaatgat ttaagaaata
      661 ttaaagaacc cgcaataatg tttattgaca atgatttttt gtgcagcgaa gaaaagattg
      721 ttgccttcat cgatattgta aaagagctgg atttaaaaaa gaattttatt atgtacggct
      781 ctacaaaagg ggtaatacaa tacgaaaaat atttaaagct attatcagaa atggggctta
      841 aagctctgct tgtaggatat gaaacataca gcgatgatga attgtcaaaa taccacaaga
      901 gctcatcagc agacgataac tttaaagctt cagacatatt aagaaagaat aggatagatg
      961 tgtgggcttc ctttatggct caccctgact gggataaaaa tgacttcagg tctcttagaa
     1021 aatatattaa aaagcttaaa cctcaaatta ctactataag cccattgact ccctttccca
     1081 accttcccat gtttaaagaa tttgaagaca gacttttgtt cagcagggag gattacgaaa
     1141 gatggagctt tggccaggtt atgattaaac cgtcaaagat ctctttaaaa gactactatt
     1201 atgagctttt gaagactaat ttatatgtta atgtttttat aaataagaat acggaaatga
     1261 ttaaaaagta tggtattatt aatattttta gaattttaaa gggttcatta aaggcagtta
     1321 aaaagtatat ggcattaatg aatgaataaa aaaaccacga aaccgtggtt tttattcatt
     1381 atacttattc ttttctttag aaaatcttcg gatatttttc atgccgccca catctttaaa
     1441 cattctgttt aacttttgtg tttcaatctg cttgaaattt aaaccctcat aatctgcttc
     1501 ttttttcagc ttgaaatagc tttcaagtct ttcttctgac aggctgcctg attccaccgc
     1561 ctttttaact gcacatcccg gttctctggt atgggtgcag tcactgaact tacacatctg
     1621 cgacaattca tctatgtcag aaaaggctct ggaaaaatca gcactttcaa ttcctaactc
     1681 tctcatgccc ggagtatcaa taacaatgcc tccttcagga agaagaatca gttctcttct
     1741 ggttgtggtg tgtctgcccc tgtcatcgtt tcttatttct cttgtttcaa agatactctg
     1801 acccataagc ttattgatga gagtggtttt tcctacgcct gaagagccta tgaaagctac
     1861 agtacagccg taattaatat atttttttat actctcgtat tcttcagcca ggcttgatgt
     1921 aaccaatata tcaagtccta tagcaatacc gctcagttca agaaaaatac tgtcaatatc
     1981 ggtgcataga tctgattttg tcaggactac aaccggtatt gctccgctgt cccatccgat
     2041 ggctatgtaa cgttccagtc ttcttaaatt gaagtctctg ttaagggaca tgcagatgaa
     2101 taccgtatct acatttgcag caacaacctg atcatccttt gatgtgcctg cagcctttct
     2161 tataaaggcg gatttcctgg ttaaaatatg gtggataaca gaagtacctt cattttcatc
     2221 cagcataaca aaatccccta cagccggata atctgatata tttactgcat tatatcttaa
     2281 ctttccagaa atttgcgctt taaattctcc gttttccgtt acagcagtgt ataaaccttt
     2341 atcctgtgaa ataattcttc ccaggctgaa gttatacatt tgtgattcat taatgaacct
     2401 gtcattcagt ccataatctt ttaaattaat tttgctcaat ttgtcctcct gaatattatt
     2461 taccggagga ttacccccga ttgtgctgat gaagttttaa aaaagtaaat cataagacaa
     2521 cactcctttc agatctaatc tgcataataa agcctgctaa atgattttac aataagaatt
     2581 tagaataatc aataaattta atcaaatcga ctgtggggac agtaaagatt ttttgttatt
     2641 atttcctata atagattctg ataattgtgt aataataaaa taaataattt aaactaatta
     2701 aagaaaggga gtattatgga taaaaatttc gaggaaataa aggaagaatt cataaaggca
     2761 agtttagatg aaaaaataaa aatatactct accacacagg ggttgacagt agaacagttt
     2821 aaagaattat tggcatattt ccctataaag tatttagata agctcgagga agccgtaaac
     2881 agcctgtagt atgacctaag ccaacttatt ggaactatgt tggacacaca ctaaagtgag
     2941 actgtaaacg gggctggttc ttatatgaac gtccctgtga tattctctgt gtaaagagga
     3001 atggctgcgg atatgaataa aacaccttaa tttggaaata attccattga ggtgatttta
     3061 tgattaagga taacaaatta agaaaaactt ttataaaaga agcgttgggc agcaggaatt
     3121 atttaagcta cagaacagat ttaatgcttt tgaaaattat tgccagcttt gcagtattta
     3181 taacagtata ttttatctac ctggacctgc ttttgtcttt attgacggca ttattggttt
     3241 ttgccgtttt tacaatgaca aataaagtaa atgttgacaa aaagaacgaa aaaggaaggg
     3301 aacttttatt taaaaaagca aaaaaagaat attttttatc aaaaatcgaa gagattaata
     3361 aaagtgattt tgaaatgctc atcaagcttt ttttcaaaaa cgagggatac aataatatta
     3421 ttaaaaaggg gaaaggattg tatttggctg aaaaggaagg ctatataacc tgcattaaag
     3481 tttttaagct ctttgagggt attgaagttg aaaagctcga tattagaagc atgctgacat
     3541 ttatgggaaa ctcaaatata aaaaatggct ttttagttac tacatccgac ttgagtgaag
     3601 atgctgcaaa actgatagaa aagtttaaag acaggtatga aataacaata atcgatattg
     3661 aaggtatgta taacctggca gacaagtaca atatgcttcc cgaggattca ttttactata
     3721 aaagaatgag cgaagaaaaa attactacca aaaagaatgt aagggaaaat gttttaagta
     3781 taaataagat atatctgtat atagctgccg ctattttctt ctatatgtca tcagtctggc
     3841 tgcctgaaaa caaattagtt atctatattt cgtatttctt tattataatg acaattatca
     3901 atgtattcta ttttttagca aataggtata gtaacaagaa attcaataaa gtagaataaa
     3961 atgattattg tttcatttat ggggtataag atagataaat atattaagtg gagataatag
     4021 atgagagata attttatggt acaaaaacag ttagagcaca gaacaatacg tgaatttaaa
     4081 aatgaaagca tacccaaaga cctgttcgaa caacttattg aagtagcaaa aagaacacca
     4141 acatcaaacg gaatgcaggc aagttcaata ataagggtga cagacccgga aaagaaaaaa
     4201 gaaatagcag ctgtatgtaa acaggaatat gtggcaagag tacctgaact cttaattttt
     4261 gtagctgacc aatacagaaa ttacaggatt gcagatgaca aaggcggata tgaaccaagt
     4321 gcaagggata tggacaggtt ttttcaaggt tttacggatg catgcctaac tgcccagaat
     4381 atagttaatg ctgctgaagc ttctgagctg ggcgtagtat ttttcggcag tattttaaac
     4441 gacgcggaaa gaataattga aattttggaa ctgcccgagc ttactatgcc tatagtgggt
     4501 cttggcatag gttatcccaa tcaggaccct cagttaaaac ccaggatgag tatggaactt
     4561 cgattatttg aaaacacata taagaaattt gacaattacg ttgaggtaat taaagaatat
     4621 gatatagaaa tgcaaacata ttatgacctg agggaagcta ataaaagagt agacagcttc
     4681 agcaaccagg ttgtcacaag gcttaaaaac cttattccaa agcgttcaga catgcttaaa
     4741 ataatgaaaa aacaaggatt tgacctgaat actgattaac taataatagt cctgagtgtt
     4801 tttcgggact tttttaccgg ccggggagac atattgactg cctttaattt atgcacataa
     4861 agttatttcg tgaaaaagat ttttttgttg tatttttaac aaatgtatat tataattttt
     4921 ctatatatta ttaaattacg gaggatgtta tgaaatttat agctacagtg ggattatcca
     4981 ataaacacgt tcatttaagc aaagagcata ttaatatttt gttcggagaa gggcatgaac
     5041 ttactcccat gaaggatctg agtcagcctg gacaatatgc ctgtgaagaa aaggttgatt
     5101 tggtcgggcc aaaaagaaca ataaagggtg taagaatttt aggacctgca agaaaagaaa
     5161 cacaggttga agtatcattt tccgatggat ttacactggg tcttgaagaa gttcccgtga
     5221 gagattcagg aaaaacagag ggcacaccgg ggttaaaact tgtgggacct aagggagaag
     5281 tggagcttga caaaggtgtt atagcagcag caaggcattt acatatgcac acttctgatg
     5341 ccgagaaata cggactcaag gacaaagaca ccgtatcagt taaagtaaac ggtccaaggg
     5401 gtttgacttt taacaatgta ttggtaagag taaatccgga atatgcattg gacatgcacg
     5461 ttgatattga agaaggaaat gcagcaggtc ttaaaaacgg tgacgaaata gaggtaatga
     5521 tagattagtt tacataattt aaattatgta aaccnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnngtgacg aaatagaggt aatgatagat tagtttacat aatttaaatt
     5701 atgtaaacca ctgtataaaa taagtgcttg ttataactag tcttctaact taacttattg
     5761 ttataaaaat ataaagttat catatagaga atgaaatacc taatgctgca agaaaaagta
     5821 ttttatgaca gatagtagaa tagaatatta aaaacaagat ctaaatatgg tgggaagaag
     5881 cggaagagat atattcttct gcttttttat tgttttaatt atatataaga tggtataata
     5941 aaattactat attttttgga ggcattatgg attttttacc tgttaatatt gaagatatga
     6001 aaaaacgggg atgggatgaa tgtgattttg tatttgtaac aggggattct tatgttgatc
     6061 attcttcatt tgctgtaggt attttatcac ggctgcttga gaggttcggt tacaaggcgg
     6121 gaatcatacc ccagcctgac tggaagaatt tatcttcatt cagagtatta ggaaaacccc
     6181 gtcttgcttt tcttgttaat tccggaaata tagattcaat ggtcaaccat tacactgttt
     6241 ttaagaataa aagaaggtca gacgcatatt ctccgggagg gaaagcagga ttaaggccgg
     6301 acagggctgt tactgtatac tcaaaccgca taagagaggc atacaaggat gtgccggtta
     6361 ttatcggggg cattgaagca agcttaagaa gattcagcca ctatgactat tggagcgaca
     6421 gtgttaaaag gtctgtatta ttggattcag gtgcagactt gctggtttac ggcatgggtg
     6481 aaaaagcttt gatagaaata gctcaagcat tggaatccgg tattcccatt gaagaaatca
     6541 catttgtaaa tggaacagta tataagacaa aagacaggga tcgtccatat tctccaatat
     6601 ttttaccatc ctataacgaa gttgtatcat caaagaaaaa attcggtgac agctttaaaa
     6661 tacaatatga gaatactgac tccaagagtg cagggacatt agtggagcaa tatgatacag
     6721 tttatgttgt tcaaaatcct cccatggaga ttttatccca gcaggaattg gatgatattt
     6781 atgacatgga ttacacgatg aattaccatc ccatgtataa aaactcaggc atacctgccc
     6841 ttaatgaagt aaaattcagc ataacaagtg tcaggggatg ctttggagga tgcagctttt
     6901 gctctcttaa cttccatcag ggaagaataa tccaaaaaag aagccacgaa tcagttctta
     6961 atgaagttga gaaaatgagg agggacaagg atttcaaagg atatatacat gatgtgggag
     7021 ggcctacggc taattttaga attaaggcat gcaagaaaca ggagaaatac ggcgcatgca
     7081 ttgacaaaga gtgcttaaca ccggaaagat gcaaaaatct ccaggttgac cactgtgatt
     7141 atataaaatt attgaggaaa ataagaaata ctgaaggaat aaaaaaagta ttcattcgtt
     7201 ccggcataag atacgactat gtaatatatg ataataatga tgaatttatg gaagaactgt
     7261 gcacacatca tgtgagcgga caattaagaa ctgccccgga gcatgtatct gaacatgttt
     7321 taaagctcat gggcaagcct tccataaaaa tatacaataa gtttgtaaaa aagttttacg
     7381 agataaataa aagtatcggc aaggaacaat atatcgtacc ttattttata tcatcccatc
     7441 ccggctcccg tttgaatgat gccatagagc ttgctgagta tataagagat atggggcata
     7501 tgccggagca ggttcaggat ttttatccaa caccgggaac actgtcgact tgtatgtatt
     7561 ataccggatt taacccaatt acaaatgaag aagtatatgt gcccagggat aaggaagaaa
     7621 gaaagatgca gagagccctg cttcagtatt ctaaaaaaga aaattacgaa ttggttttaa
     7681 aagctttaaa aatggaaaac agatacgatc tgattggata tggaccaaag gcgttaatca
     7741 aaccaaaaaa gatgccctaa ccctattttt catggttttt tatgaaaatc agcaggaggt
     7801 atttcgcaac gaaatggaga tagcctggat agtctgtaat acaattttaa taatttaatt
     7861 tcttgcaaaa tctttcatag taagatagaa ttgtaatgta attattcaaa gaggtagcat
     7921 atgaacagaa aggcaagacg acgcaaaaaa agaataagat taatgtcatg gtcattagta
     7981 ctccttgttg gagtattggc agcttcattt gttgtgaagg gtatgggcaa ggacagaaaa
     8041 aacgaagagc agccgccggc agatgatccc gtaaatgagc aaatcggaga agaaacttca
     8101 aatgaagtaa caaagcccct gcctgaacct gacgtgtcta tcagaataag agctaccggt
     8161 gacataatgt ttcatccctc ccaattggag ggggcatatg actatgccac aaatacctat
     8221 aattttgaaa actcttttaa agctataaaa aatattatag aagaagcaga tatagcaatt
     8281 gctaattttg aaggaacagc tgcaggcaat ggaacatacg cctatcaggg ttatcctttg
     8341 ttcaatgctc ccgatgaagc attggatgct attaaaaatg caggatttga catattgcag
     8401 actgccaaca accacagtct tgacacaaga aaagcaggta ttataagaac aatagaacaa
     8461 attaaagcaa ggggaatgga gcctgcagga actttcaggg aaaaacccga gacaagggtt
     8521 ttaatcaagg aagttaaggg tattaaatta gcatttatag catatacgga aatggttaac
     8581 ggtcttgaat cggttctttc acctcaggat ttggatgcga tggttaatat ccttaatgag
     8641 tcaaaaatga aggaagatgt tgcctttgcc agggaacaaa atgctgacgc aataatagct
     8701 ctcttccatt ggggagatga atattcaagg gttcaggcaa aaagacagga aattttggcc
     8761 gatatgctgt tcagagaagg tgtggacata atactgggca atcatccaca tgttattcag
     8821 cctacacaaa cattggagta tgacggcaaa acaaaatttg tagcatattc aatgggtaat
     8881 ttcttatcaa atcaaagggt ggaaaccctg gttccttacg gcttgactga acatgtttca
     8941 aaatatacag aagacggaat tataatcgat atagaaatag aaaaaaaagc cggtacccaa
     9001 gaggtatcaa ttaaaaatat taactatata cccctgtggg tttataaagg tttcaaagaa
     9061 aacgggacag cggaacatgt tgtttaccca ataatggatt atattgaagg tgctgaactg
     9121 gatgaagatt caaaagcaag aatgagcagg tcatatgcgg atacaatggc acagatgaac
     9181 ggcgaataaa cgggggaact ttatggaatt attggatcgt attagattaa ttgttacgaa
     9241 tgtaggtctg aataccatag tagatatagg tattgtatac tatgttcttt atcgtggata
     9301 cattctcatc aaagacatga gggcaaaaca actggtcaag ggaattgtgc ttttggttgc
     9361 tttaatacct atatccagta tatttgattt atacatggtg gaatttattt taaggaatac
     9421 atttcaggtt ggattaattg cattggttat tgtttttcag cctgaaatac gaaaagcact
     9481 ggaatatata ggcaggactt cctttactat atcaaatgtt gagaaaaaca ccgaaacatc
     9541 taaaaaaatt attaaagaaa tcatatcggc agcgacctcc ctgtcaaggc aaaaaatcgg
     9601 agcattaatt gttttcgaaa aacaaatcgg tctaaatgat ataatagaaa gcggaactgt
     9661 aattaacgcg aatatctcca gcggactgct gataaatatt ttcattccaa atactcccct
     9721 tcatgacgga gcagtaataa taaaggatta tatcataaag gcagcaggat gttttttacc
     9781 tcttacggat aacaaccttc tcagcaaaga tatagggaca aggcacaggg cagccatagg
     9841 gataactgaa agatccgatt caatagcttt ggtagtttct gaagaaacag gatatatttc
     9901 ctatgctgtg gaaggaaggc tttatagaaa tattcaagct gaagaaattg aaactatact
     9961 gcacagtatt tatattgaga atgaaaaggt caagattata gataagtgga gaaatagaaa
    10021 tgaagataaa agacgataaa ttagttatac agattgcctg tcttgttgtt gccattgttc
    10081 tttggatgat tgtaatgctt gtgaccaatc aaacccttga ggactcatat ccaaatgttt
    10141 ctgtaaccat aagaaacctg tcggctttgg aaaactcaaa tttggtaatc atgaattctg
    10201 acaaggaaaa tattactgta acagtaaagg caacaggatt aacagaacag ttaaacaata
    10261 taagcagcag agatttttct gcctatatag atgttttggg ttttaaagaa ggaataacaa
    10321 atgcgaaggt agaggttgtg ggtccaagcg gtgttgatat tgtaagcgtt tatcctgccc
    10381 agatagcatg taatatagaa ggagtaatat cgaaggttat ggatgttacg gttcaatctg
    10441 aaggagtaca ggccggcagc tattacaagg cacaaacagt atccaatccg tcatccgtta
    10501 agataacagg gccaagaagc gttgtaaatt cagctgaaaa ggcagtagct accataaaca
    10561 taaacggtgc aacagatgat gttattaaaa ctgttcctgt aagagtatat gatgatacgg
    10621 acacagaaat attcttgtca gcaccaacag ggaatgttaa agttacagtt ccaatctacc
    10681 caacaaaata tgcagaactt gtgccgggtg taacgggaac tcctgaagaa ggatacgagc
    10741 tggtggacgt gacagtaaaa cctgaaaggg tgagaattgc tgcaaggcag gatattttag
    10801 atacaatcaa ggaacttaat ctggaagtga ttgacattac aggtgcctac aacaatatgc
    10861 tgacagccag aaatatattg aatgcagagg gcttgatact tttggacctg gaagctgcac
    10921 cagttgtaaa tactgtgatt gaaaaaatca tacaaagaga atttaagtac acctatgatg
    10981 atattcagct gataaacaaa agcgaaaata ctgatgctgt tattgaagaa acagatgaat
    11041 ttcttacggt aaccgtgttt ggaactacta ccctgcttac tgaaatggaa aaagatgatt
    11101 taatcctgac tgcagattta tcagaaactg ttacaggttt aaatactgtt aatatagatt
    11161 atgtttccga atttaccttt aaggaaataa tattgagcaa aggaaccttg aatttggaaa
    11221 ttattgaagc agtagaacct caggaagagg aataagagat gcaaaaaata aattttaaca
    11281 gcatggatta tcagggaatg ctggaagcag tccttcaggc tgctcaggat gctataagtg
    11341 ttgtagatga aaaaggggag catatccttg ttaatcctgc ttatacaaga ataacgggca
    11401 ttgattatga tgaaattatt ggaaagaatg ctttgtatga cattgaagac ggggaacaaa
    11461 gtgtgcatat gaaggttctt tcaacaaaaa aacctgttga aaacacaaag ctaaaattaa
    11521 gaccctccgg aaagaccgtt gttgcccagg cggcaccgat aaatgttgac ggtgaaatga
    11581 agggaagtgt ggctgtttta cacgattatt caacaataaa ggatttaaca gataagctgg
    11641 aggaagcgga aaaaaaatta agacagctta catataaata taaacccgag gatataatcg
    11701 gaaactcaaa agccataaat gaagtaagaa atctaataaa aaaagctgcc aaagttccgg
    11761 ctactgttct tttgagaggt gagagcggca cgggcaaaga attgttcgca aactcaatcc
    11821 atgctcaaag cacccggtcc cgtaaaaatt ttataagagt aaattgtgcc gcacttactg
    11881 attctttgct tgaaagcgag ctgttcggct atgacgaagg ctcatttacc ggtgcgctta
    11941 aaggaggcaa aaaaggtctt tttgaagaag ccgacaaggg caccatattt cttgatgaaa
    12001 tttctgaaat aagtttaaat actcaggcga aacttttaag ggttcttcag gaaaaagaaa
    12061 taatgagggt tggaagcagt aagaatattc ccgtagatgt aagggtaatt gcagcaacca
    12121 attcagattt gacaaaatta gtaagtgagg gaaaattcag agaagattta ttttatagaa
    12181 taagcattct tcccataagc atacccccac taagggaaag gccggaagac attcccctgc
    12241 tggtcaaaag ctttattgta aaatttaatg atgaatacgg aagaaatatt attgatataa
    12301 cagatgatgc gctggctgtc ttaaaatccc atgactggcc tggaaatgta agggaattgg
    12361 aaaatattat aggcagggca ataatatata tgaacttaaa cgaaagaatt attgacaggg
    12421 cgcatttgcc ccatctgaat gccaataagc aaaatacggg tattgcagta acaattgatg
    12481 aaggtgtaca ggacctggaa agtattttag ataaaacaga aaatgaatat atcactaaag
    12541 tttacttaaa atttaataaa aataaaaccc ggacagccag ggagctgggt atatctttaa
    12601 ggtccttgta ttataagtta gagaagtatg gtatcttata atctgcaaaa tattgcaaaa
    12661 tttttaatat tttgaaaata attgcatgca aaaaatttca agcaaataat aataaaaacc
    12721 aatcaatcgg tgaaatttca acgattgatt tttctttatt cttggcatat tctttgcaag
    12781 acatatatat cgaggtgaga acgtaaatgt atagattcga taaattattg aaagaatccg
    12841 gaaacaccca gccaaaaaaa atagctgttg cagcccccca tgacgatgac atattgaagg
    12901 ctgtaaaatc agcagttcta aagaatatct gcattccgat cctgattggc attaaagata
    12961 aaattattga attatcaaag gaaataaact ttaatcttga taacattgag attattgaaa
    13021 ctaatgatga tatttcagct gcaaggacag ctgtatcttt ggtatcatcc ggcaaggcag
    13081 atatcgtaat gaaaggcttg ttagatactt ctattatttt aaaggctgtt ctggataagg
    13141 agatcggact taggacaggc agtgtcttaa gtcatgcagc agtttttgaa acagataaat
    13201 accataaatt tttgattatt actgatgcgg caatgaatat tgcacctaca gcatctgaaa
    13261 agaagcaaat aattgaaaac acacttcctc tatgccgttc attgaatatt gaaaatccaa
    13321 aagttgcggt aatatgcgcc aaagaaaagg tatcagacaa aatgcaggct actctggacg
    13381 ccgaaatact tgtaaacttg tacaaagccg gcgaaattaa aggctgcata gttgagggac
    13441 cctttggact tgacaatgca atttcaaggg aagcagcaac tgtaaaggga gtgaagggag
    13501 aagttgccgg tgatgcagat gttttgttaa tgcccaatat agaagcagga aatgtaatgt
    13561 ataaaacatt gacatatttg gctgattcta aaaatgcagg cattatttta ggtgcaaagg
    13621 cacctgtcat tcttacttca agggcggatt cagatgaggc aaagctttat tcaattttat
    13681 tggcagtaat ttgttcaaag gagagcatat gaaacaatta ataattaatc cgggttcaac
    13741 atcaacaaaa atagcagtat ttgatgatga aacaatggtt ttcgagaaaa caataagaca
    13801 tacaaatgaa gaactgcagc agtttaacag ggtttcagac cagtttcaat tcagaaagga
    13861 catgattatt cttgcattga aagaaaacag catatcatta gatatgctgg atgctgtagt
    13921 aggaagggga ggtttgataa aacctgtcga aggcggaacc tatacagtaa atgacaaatt
    13981 gattgaggac ttgagaaaaa atgtaaaggt tgagcatgct tccaatctgg gtgctccttt
    14041 ggcacgttcc atagctgatg aagtcaatat cccgtcattc atagtggatc ctgtggtagt
    14101 ggatgaaatg gaagatgttg caagggtaag cggacaccct gattttgaaa gaatcagcat
    14161 atggcacgca ttgaatcaaa gggctgtcgc cagaagagct gctatggaac aatttggcag
    14221 gaaatatgag gaccttaatt ttgttgttgc acacttagga ggcggaatat ccataggtgc
    14281 acataaaaag ggaagagcag ttgatgtaaa caatgctctt aacggagaag ggccattctc
    14341 tcctgaaagg tcaggttccc ttccaatggg acagctggtc aagatgtgct tcagcggtct
    14401 ctatactgaa gctcagataa ataagatgat ggtaggaaag ggtggaatca gtgcacattt
    14461 aggaacaaac agcgcaaagg aggtaagtga cagggcccaa gcaggagatg aaaaagcaaa
    14521 gctcatatat tctgcaatgg cataccaggt tgcaaaagca ataggcgagt atgcagtagt
    14581 tcttgacggg gacgttaatg caatattaat aacaggcggc atagcttatg acgaatattt
    14641 tgtaaaaatg attgaagaca aggtaaaatt catagcaaag gttatagcct atcccggaga
    14701 ggacgaacta ttggctcttg ctcaaggggg attgagagta ttaaaaggta ttgaaaaccc
    14761 gaaaacctat atataagaca taataaatat tccggaagga gggatatgat gcatgacaag
    14821 agattggtta ttgttatagg tcattatggc agcggcaaaa ctgagtttgc tgtaaactat
    14881 gccataaaaa tgaaggaaat atatgatagt gtatctattg cagatttaga tatagttaat
    14941 ccctatttcc gctccaggga aaagagagag ttgtttgaag aaatcggaat aaagctgttt
    15001 gattcaagca taagaaatac agcagttgat atacctgccc ttccggctga gctgatggga
    15061 gttattttaa atcaaaatgt aaaatcaata cttgatgtag gaggagaccc ggtgggagcg
    15121 agagttctag ccaggtattc agaccaaatc aagaatgtcg agtatgatat gctgtatgta
    15181 attaacggaa acagaccatc aacaagcacc gttgaaggag ttttgaaata tttaaaaatg
    15241 attgaagata catcaaggtt aaagataaca gggctggtaa acaataccca catgttaaaa
    15301 tctaccacag ttgaagatgt ggaattcggt catgaactaa ctaaaaaggt atcatgggaa
    15361 acagacattc ccataagata tgaagctgtt ataaaagaga cagctgacaa aataaagaac
    15421 caagaaataa tagaaaagtt gttccccata aatctatata tgagggaaga atggatgagt
    15481 taaaggaggt tttataatgg caaaaggtaa ggttacattt aatgacaatg catgcaaagg
    15541 ctgtgaactt tgcgttgcgg tttgtccggt aaaaattatt aaattgaaca aagagaaaat
    15601 aaataccaag ggttacagcc ctgcccatgt aattgatcaa gaaaaatgta ttgcctgcgg
    15661 gaattgtgca attatgtgtc ctgattcagt aataaaagta gaaagattat agtggaggta
    15721 gattatgtca aagatattaa tgaagggcaa tgaagctgtt ggagccgctg ccattaaagc
    15781 aggctgcaaa tttttcttcg gttatcctat aaccccctca agtgaaatac ctgaatatat
    15841 agcaagagag ttaccggcag ccgggggagt ttttctgcag gcagaaagcg aaatagctgc
    15901 aataaacatg gtttacgggg caggcggatc aggtgcaaga gttatgactg catcttcaag
    15961 ccctggaatt tccttaaaac aggaagggat tacctattgt gcgggtgctg aaattccgtg
    16021 tgttatagtt aatatgatga gaggcggccc gggacttgga agcatacagc cgggacaagc
    16081 agactattat cagtcaacaa gaggcggcgg aaacggtgac tacagaacac tgtgctatgc
    16141 accggcaagc atacaggaac tggttgatta tgtcatgtta gcatttgaca aggcagatta
    16201 ttacagaaat cccgtgctga tatgcgggga cggaatgatt ggtcaaataa tggaagctgt
    16261 tgaatttaaa gatcctgaac cgattgagct gccacctaaa gattgggcga caacgggaac
    16321 aggcggaaaa agaaaacctg ttgttgtaaa ctctctttat ttagaggcag aagaactgca
    16381 ggaacacaac aaacacttgg aaaagaaata caagcaaatg gaagaaaatg aagttctata
    16441 tgaagcattt ggtttggaag atgctgatgt tgtgtgcgtt gcttacggta ctacagcaag
    16501 gatagtaaaa acagcaataa aacaggcaaa agcaaaagga ataaatgtgg gtatgataag
    16561 acctattact ttgtggccat atccctacga cgtattcaag gaaattggag ataaagccaa
    16621 gggaatactt acagtcgaga tgagccaggg tcaaatgact gatgacgtaa aaattggtat
    16681 tgccggaaga ctgcctgtat atttcaacgg cacacaaggc ggtatggttc caacacccgg
    16741 aaacattttg gcagatattg agaaaatagc aggaggtgca aaataatgac aattgtatat
    16801 gaaaaatcaa agggcttaac agatacacct tttcattatt gccctggttg tactcatggt
    16861 gttattcata aactggttgc agaatctttg gttgaattag gagttttgga tgatgcaata
    16921 ggagtagctc ctgttggatg ttcagtactt gcttacaaat attttaactg cgatatgcag
    16981 gaagcttctc acggaagagc accggcagtt gcaacaggca tcaagagagt ccatcccgac
    17041 aaggttgtat tctcatacca aggtgacggt gatttagcat caataggaac tgcggaggtt
    17101 gtacatgcag cacatagagg agagaaaata accacaatct ttgttaacaa cgcaatatac
    17161 gggatgaccg gcggacagat ggctcctacc acattggttg gacaaaagac aaccacatct
    17221 ccatttggaa gagatgaggc acattgcgga aaacctataa aaatgtcaga actattggcg
    17281 acaattgatg gagctaaatt tgtagaaaga gttacagtag atactccggc aaatatcaga
    17341 aaagcaaaaa aagctataaa gagagctttt gaatgtcaga taaaaggcga aggttttgca
    17401 atcgtagaag tattatcatc atgcccgaca aactggggat ttactccttt aaaagcattg
    17461 gactggctga gagataatat gataccatat tatcctttag gaaacttcag agattttggg
    17521 gagggaaaat aatatggaac aaaaagtaat tatggccgga tttggcggtc aaggtgttat
    17581 ggctatcggt aaattattgg catatgcagg aatgattgaa gggaaaaatg taacatggat
    17641 gccttcttat ggacctgaaa tgagaggagg tacggcaaat tgtgcagttg ttgtatcgga
    17701 ggaagaagta ggctctcctc ttatttccaa agacgggact gcagcaatag ttatgaacct
    17761 gccttctatg gttaaatttg aaaaagaact tgttcccggc ggaaaactta taataaacaa
    17821 atcattgata gatgttgagc cagaaagaaa agatctggat gtttattata ttgaagctaa
    17881 tgagctggca ttggaattag gcaacagcaa agttgcaaac atggtaatgc ttggagcata
    17941 tctggaaatt accaaagcag ttgaaataga atcagtatta caggcttttg taaaagtttt
    18001 cggtgaagat aaaagagacc tggttccatt aaacaaggaa gcattgctga aaggagccca
    18061 ggctgcaaaa aaatagggga cggatatttt aatataaaaa tgttaaaata agcaaggagg
    18121 actcctctgc ttcatggcaa gcgatgtgca gaggaatgtc cccggttaca gaaatgttta
    18181 caagttagta tttaagaaaa aggaggaagt aaagtggaaa aaaaggacat caaatgggct
    18241 gaattaggat tcaattatat taagaccgac aaaagatata tttcatactg gaaggacgga
    18301 aaatgggatg aaggacagct tactgatgat aataaggtac atatcagcga agggtctaca
    18361 tgcctgcatt acggtcagga atgcttcgag ggactgaaag cttattctaa taaggatggt
    18421 ggcatacaac ttttcagacc tgatgaaaat gcaaagcgta tgcaaaaaag ctgtgaaaga
    18481 gtaatgatgc ctatagttcc tgtggaaaaa ttcatagatg cctgcatgca ggttgttaag
    18541 gctaacgcag catgggttcc tccatacggg tctggtgcta cattatattt gagaccatat
    18601 gtaataggcg taggtgacaa tataggggtt aaacctgcag aagagtacat attcggagta
    18661 ttctgcatgc cggttggtcc atactttaaa ggcggcatga atcctgttaa cttcataact
    18721 actgacgaag acagagcagc acctaacgga acaggaaaga taaaagtagg cggaaactat
    18781 gcagcaagct tgcattcaca ttttgaagct ggtaagaagg gatttgcaga ttgtatttat
    18841 ttagatcccg caactcatac aaaaatcgat gaagttggag ctgcaaactt ctttggcatt
    18901 acaaaggacg acaagtttgt aacaccccat tcatcttcca tacttccaag tataacaaga
    18961 atttcattaa tgcaggttgc caaggattac ttgaacatgg aagcagttga aacagaaata
    19021 tacattgatg aaattgataa tttgaaagaa gcaggagcct gtggtacagc ggcagtcata
    19081 actccgatag gcggaattat gcacaatggc aagctgcatg tattccacag cgagacagaa
    19141 gtaggacctg ttactaaaaa gttatataac acattgtacg gaattcagat gggcgacata
    19201 gaagcacctg agggctggat ttacaaagta atttaacatt tccctccaat gggcactaag
    19261 tataaccaaa catacttaat gccttttttt tgttcttcaa aatttaactc ttttatgata
    19321 aaatcaaaaa gaaggaaagg ggacacaagg agaacatatg aagcaaatca ttagagaagc
    19381 agcttacaaa acaggaattg aaacatttgg tataagcaat actgcagact attcctatat
    19441 gaaagacatg cttaacagca gaaaagaaaa taatcaggac tgtgaatttg aagagcagga
    19501 tataaataaa agattaaatg caaaaaattt gtttccagaa ttaaaaagca taatagcaat
    19561 aggcattcct tacggtgagg gctacaagcc tccttcaagt ctaaataagg gtttattgag
    19621 cgtgtcctgt catggagagg actatcataa tactgttact tccttattgc ttaagcttgc
    19681 tgaagaaata aagcgccata gtaagtttga atttcttccc gttgttgata catcctttct
    19741 tatagataaa gaaatatgca aaagtgccgg tattggaaac tatgggaaaa acagtctcct
    19801 tataaataaa aaggccggat catttatgaa tctgggttat cttttgactg atattgaaat
    19861 tccggcagat actattaagg aagaagatat atgtggtaca tgcattttat gcgtaaaggc
    19921 ctgtcccaac aatgcaatat ttgaaaaggg cggcattaat cccaaaagat gtgtgtcata
    19981 tttaacacag accaaaagtt acattccttt ggaattcaga gaaagcatgg gcagacaaat
    20041 atacggctgt gatatttgcc aaattgtatg ccccttgaat agagacctgc taaataataa
    20101 aaccagggac gcatattccg atttagaagt tgatttggaa gagcttctta tgatatcaaa
    20161 cataggcttt ataaaaaaat acggaggcag tgcagggggc tggaggggca gaaacatatg
    20221 gaagaggaat gctgttatag ctgcaggcaa tcttaaaata tattcaatgt ttcaaaccat
    20281 taaatcagag cttcaaaatc cctcagatat gataaagatg tatgcagcct ggtctttgtt
    20341 gaaattaaat aaaccgtcag ctgaaaacat cctatacgat aatttaaaat acgaaaatga
    20401 caatgtgaaa agtgaatact taaagctttt ggaggaaaaa ttatgatagt atgttcttgt
    20461 gaaattgaaa ttttaatata tgagtcaaat tctctgaagg agaaaagaca ggtagtaaag
    20521 agcataattg agaggattag gtcaaagttc aatgcttcca tagccgaggt gggttataat
    20581 gagttgtgga acaggtcctt gctgggtctc gcagttgttt caaataataa aagattatgc
    20641 gacgcttcca tatcaaagat aatttctttt atagacaatg acgaaagagt tgaaataata
    20701 aattgccgca gggagatggt ataatgacaa aggcagaaaa agtgtatgaa ttattaaaag
    20761 aagcttatcc ggatgcaaag tgcggtcttg aatataaaac cccattacag cttttgatat
    20821 caacaatttt aagcgcccag gctacagata aaagtgtcaa cagcgttaca gaagaattgt
    20881 ttataagata tcccgacctg gatagttttt taacattatc ccaggaagat cttgagcaaa
    20941 agatcaaaaa aatcggatta tacaaaaata aatcacaaaa tattttcaat atgctcagag
    21001 tgttaaagga caaatataat ggggaagttc ccaaaagtat ggacgagctt ttgtctcttc
    21061 ccggagtggg aagaaagacc gcatcagtag ttcttgttga agctttcagc attcccgcat
    21121 ttcctgtaga cactcatgtt ttcagagtaa caaggagaat aggccttgcc gtggaaagca
    21181 cagccgacaa ggtatccgat gaaatgatga agaagcttcc aaaatacaag tggcatctta
    21241 tgcaccacct ttttataaca cacgggagaa ccacatgcac agcccaatct cccaaatgta
    21301 atatttgcat ggtaaatgaa ctatgtaaat attttaaaaa acaagacaaa caatagaaaa
    21361 ccttgttgta tttggcctaa atataatcta tcatgttgtt ggatatcaat tatattgttt
    21421 tatcgggaga aaaaaatgtc attaaacatt gtactatttg agccagaaat accgcaaaac
    21481 acaggcaata tagcccgcac ctgcgcagcg acaggttcaa agctgcattt aataaagcct
    21541 cttggctttt cagtgaatga taagtattta aaacgtgcag gactagatta ttggaatctg
    21601 gtcaatataa cctattatga aaactttgaa gaatttaaga aaacaacagg taacaataaa
    21661 gtttatattt caacaacaaa agaaagcaga ttttatacag atgtcactta ttttgacgaa
    21721 tgctatctaa ttttcggaaa ggaaactgca ggtcttcctg actatattca cagggaatat
    21781 atgcaggaca ggataaagat tccaatgata cagaaggtaa atgccaggtc cctgaacctg
    21841 gctaattcag ttaacatagt aatttatgaa gctttaagac aattaggatt tccgggtatg
    21901 atataggaga atttattgat gtagctgatt agcagccttg gtaggaagtg actattcctg
    21961 atgattaata tatacttaaa gtaatttaaa tgtcacttat tacttaagcc aacttcttcg
    22021 aaagctgggt tagaaaagct gctttgggtt atcctaaatt taatataaat ttaacaatat
    22081 taagaaataa ttgttgattt atttatagaa gtcttatata ctataagtaa ttaaatactt
    22141 ggatgacatt cgcgggagag actgttaatt gccagacagc gccgaaggaa ataagttatg
    22201 attagccata ttgtaatgac tatcgcaggc aaaaggaccg tggattgaca aatctctgga
    22261 aagcgcaagc accgaagggg caattctaaa cgaagaatct ctcaggttaa ttaacagggc
    22321 aggaggcaga atgttttttg attttgcttt ctgcccgtaa ttatatttat acatggagaa
    22381 aaaagtgatt attacaaaca atgattgggt attatacaaa tatagagaaa agtttaatgt
    22441 tgtttacatt gacggaacat ttaaagatgt tttgattgca gtaagggaca ggctccatat
    22501 gggatacgag ctgttgactc atcctttggg aggcagtgtg aaaccgggcg aaacacctta
    22561 taaatcaata atggtcagtg atgataaaaa aagtatcagc ttttcttcaa tttacacgat
    22621 tgaaaatgca ataattacat atgataagtt caataagaat aagttgaaat ttataaatga
    22681 caagatagct gaggatttga aattaattga tttaactgtg ctggaaagcg cattaaatat
    22741 ataactatag aaaaaattta aacggaggtg aaatttttga aattagaatt aggctatatt
    22801 tttattaagg atattcagtt ttctgacgta tccaaggtag aagatggtac attgtatgtc
    22861 aacaaggaag aagtaaaagc attaattcta gaagatgaac atttcaaaaa agcagaagta
    22921 gagctggcca agccaggaga aagtgttcgt ataatgcctg ttaaagacgt tatagaacca
    22981 agggttaaag ttgaaggacc cggcggaata tttccgggaa tgatcagcaa agttgaaact
    23041 gtaggctcag gcaaaacaaa tgtcctgaaa ggtgcagcag ttttaactac aggcaagatt
    23101 gtcggtttcc aggaaggtat tattgatatg accggtcccg gagcagatta cactcctttc
    23161 tcaaaacttt gcaatttagt tttagtgtgt gagcctattg acgggttaaa gcagcacgag
    23221 catgaaaaag cattaagatt tgcaggatac aaggtagctt cataccttgg cgggcttgca
    23281 aaaaaattaa ctcctgacca ggttgaagta tttgaaacac caaccttgac agaagggtta
    23341 aaaatgtatc ccggccttcc tagagtagca tatgtatgta tgcttcaaag ccaaggactt
    23401 ttgcatgaca cctatgttta tggaattgat gcaaaacaga cattaacaac atttatttat
    23461 cctactgaga caatggatgg cgctatagta agcggtaact gcgtatcagc atgtgataag
    23521 aatactacat atcaccatct gaacaatcct gttattgctg atttatttgc agagcacggc
    23581 aagacattaa attttgttgg tgtaatcata tctaacgaaa atgtttatct tgcagataag
    23641 gaaagatcat ccaactggtc agcaaagctt gcagaattct taggagtaga tggtgttgta
    23701 gtttccgagg aaggtttcgg aaatcctgat acagacttga taatgaactg caagaaaatt
    23761 gaatcaaagg gtattaaaac agttcttata actgacgaat atgcaggaag agacggtaca
    23821 tcacagtcat tggcagatgc agacgcattg gctaatgctg ttgtttcagg aggaaatgca
    23881 aatgaagtca tagagctgcc tcctatggaa aaaataatcg gtcacgttga agttgcagat
    23941 ataattgccg gaggatttga cggaagctta cataaggatg gaagcataac agcagaactt
    24001 caggttatta caggtgctac aaatgaaatg ggcttcaatg aactcagtgc cagataggaa
    24061 gggaggagaa aataatgctg aaaattgttc attatattaa tcaattcttt gctaatgtag
    24121 gcggagaaga aatggctcac attcctgctg aagtgagaga aggtgtagtt ggcccgggac
    24181 ttgcattcca gcaagcattt aagggtgaag ctgaaattgt tgctacgatt gtatgcggcg
    24241 actcatattt caacgagaat atggatgaag ccaaatcaac cgtaattaaa atggtaaaag
    24301 aataccaacc tgatttattt attgcaggac cggcatttaa tgcaggaaga tacggagttg
    24361 cctgcggtac aatcgcacag gcagtaaagg atgaacttaa cattcctgtt cttacgggaa
    24421 tgtatgcaga aaatccaggc gcagatatgt tcaaaaacac catctatatc gtatctacaa
    24481 aaaattctgc tgcaggtatg agagatgctg ttaagaagat ggctccactt gcacttaaac
    24541 ttgcaaaggg agaaaagatc ggtgcatccg cagaagaagg atatatgcca aacggcaaaa
    24601 gagttaattt ttttgaaaaa gatagaggct caaaaagagc agttaaaatg cttttggcaa
    24661 aattagccga caaggaattc gaaacagaat tacctatgcc tgactttgac agagtagctc
    24721 caaacccggc tgtaaaagac attacaaaag ctaagatagc attggttact tcaggaggaa
    24781 ttgttccaaa aggaaatccc gatcatattg aatcatcaaa tgcatcaaaa tacggcaaat
    24841 atgatataga cggagttatg gatttaaccg aagaaacata tcagacagct cacggcggat
    24901 atgaccctgt atatgcaaac ttggatgctg acagagtatt gccggttgac gttttaagag
    24961 aatttgaaaa agaaggaaaa atcggaagtc tgcacagata ttactattct acagtaggta
    25021 atggtactgc agtaaagaat gcaaaaggtt ttgcagcaga atatgctaaa gaacttaaag
    25081 ctgacggagt tgacgcagtt atactaacat ctacgtgagg tacctgtacg cgttgcggtg
    25141 caactatggt aaaagaaata gaaagagcag gaattcctgt tgtacacatg tgtacgatag
    25201 ttcctatttc attgacagtt ggagctaaca gaattgttcc aacaatagca attcctcacc
    25261 cattagggaa tccggcatta tctcttgaag aagaaagaga tataaggagg aacctcctta
    25321 caagagcttt aaaggctttg gaaacagaag ttactgatca aacagtattc gaaaaataag
    25381 attcgcacaa gttcccaatg taatagactt aaaagatatg gggacgcacc tctgctgata
    25441 aacaattctt tgagtgtaag aggaatgtcc ccaaatatag aaaattggag aaccggactg
    25501 cgaacagtaa aactatatgc agagaaggtg gaatatgagt aaaatttatg atgttttaat
    25561 tatcggcgca ggacctgcag gattatcagc aggattatat gcaggaagag ctagacttag
    25621 cactttgata attgaaaaag aaaaggatgg aggacaaatt gttcttacat cagaagttga
    25681 aaactatcca ggatgtcttg aaggtgaatc tggacctaca ttagtagaca gaatggctga
    25741 acaggcaaag catttcggtg ctgaaaaagt ttatgatgaa atattagaag taaaacttga
    25801 tggcaaagaa aaagttcttg ttgggaaaaa aggcgagtat gttggaaaaa ctgtaataat
    25861 tgccggcgga gcaacatcaa aaccaatagg atgtcccggt gaaaaaacat tcacaggcaa
    25921 gggagtttct tactgtgcaa catgtgatgc gtcattcttt gaagactttg aagtttttgt
    25981 agtaggcgga ggagatacag cagttgaaga agcaatgtat cttacaaagt ttgcaagaaa
    26041 agtaactgta attcacagaa gaaatgagtt aagagctgca aaatctattc agcaaaaagc
    26101 atttgcaaat cctaaaatga actttatgtg ggattctgta gttgtagaaa tcaaaggcga
    26161 cggtttagtt aattccatgg tagttaaaaa tgtaaaaaca ggtgaattaa ctgaaatagt
    26221 tgctgatgaa gaagacggca catttggcat tttcggattc attggttatg aaccaaaaac
    26281 taagatattt gaaggtatat taaatatcca aaatggttat cttgtaactg atgaggatat
    26341 gaaaactaat attgaaggtg tttatgctgc cggagatata agagttaaaa gcttaaggca
    26401 ggttgtaact gcagcggctg atggtgcaat tgcagcagta caggctgaaa aatatatcga
    26461 atcattatag gaggtggtta gttatgttat tgcttgataa agaaacattt gaacctgagg
    26521 tacttaaagc tgaaggttat gtatttgttg atttttttgg agatggttgt gctccctgtg
    26581 aagctttgat gccacatgta atggctttgt cagaaaaata cggcgacaag ctaaaattca
    26641 caaaattaaa tactactaaa gcaagaaggc ttgcaatagg acaaaaaata ttaggtttac
    26701 cggttattgc agtatacaaa gatggagaaa aagttgaaga attaattaaa gaagatgcta
    26761 ctccggaaaa tgtagaagca atgattcaaa aatacatcta gtaatcactc tttaaacaga
    26821 ttaataaact aaaaaacaaa aaaataaaaa acaggaggaa aatttatgag cttattacaa
    26881 gggaaaaaag taataatcat aggcgacaga gatggtatac caggcccggc aattgaagaa
    26941 tgtgttaaaa ctgccggcgg tgaagttata ttctcatcta ctgaatgctt tgtctgaact
    27001 agtgcaggcg caatggattt ggaaaatcaa aaaagggtta aagaatttgc agaaaagtac
    27061 ggtcctgaaa atgtagtggt agtacttggt gcatctgaag gtgaagcagc aggtcttgca
    27121 gcagaaaccg taacaaacgg agacccaact tttgcaggtc cattgacagg agttcagtta
    27181 ggactctcag tattccatgt agtagaacca gaaataaaag atatagtaga tccagcagtt
    27241 tatgatgaac aagtgggtat gatggagatg gtattggatg ttgatgacat tattaatgaa
    27301 atggctccaa tcagagaaga attttgtaaa tacctataat ataaaacatt aatcaaatat
    27361 gcttcgcaga aatgcgaagc aaattttaat aacggaggtt agtatgaaca gtgtaataaa
    27421 aggggcaagc tatatactgg cttttactcc agatatggtt attcacaacg gaacaactca
    27481 aacaactgaa agaaccgtta acccaaattc agaatattta aaacaaatca aagatcattt
    27541 aagaacattt gaagaagtag taaactacat gccaaatcaa gcatacatag gaaatatgac
    27601 accggaagaa ttaggtcaat atgaacagcc atggaatgac aaaccgcttc aaggtgcttc
    27661 aagatgggga aaattcggcg aaataatgcc gcaggatgag tttatagtat taatgcaaat
    27721 atgtgacgta tttgaccttg ttaaattaga aaacgacttc ttagcagaaa caactgctaa
    27781 acttaaaaac catcctttat ttgatgaagc attattagca agaattaaac aaggtgaaga
    27841 tggtgaattg attaaaaagt tagttgaaga agaacattca gaagcattgt atcacaacgg
    27901 gcaattagta ggctgcgtaa aaagggcaca tgacatcgat gtgaatttaa atgcacatgt
    27961 tatgttggaa aacttggtat caaaggcttc taacgtattg gctttattaa atctggttgc
    28021 taaaaacaat attaataaag atgaaatcga atatgtaatc gactgctgcg aagaagcatg
    28081 cggagatatg aaccaaagag gcggagggaa ctttgcaaag gcatgtgctg aggttgcagg
    28141 attcaacaat gctacaggtt cagatacaag aggattctgc gcaggacctg ctcatgcgtt
    28201 gattgaagct gccgcattgg taaaagccgg cgtatataag aatgttgttg taagtgccgg
    28261 cggtgctaca gccaaacttg gaatgaacgg taaagaccac gttaaaaaag gacttcctat
    28321 acttgaagac gtattgggag gctttgcagt attaataagc gaaaatgacg gtgtaaatcc
    28381 tgaaattaac cttgatttag ttggaagaca tactgttgga acaggttcat caccacaggc
    28441 tgtaattaca tcacttgttt cttctcttga aagagcagga atgaaaataa ctgatataga
    28501 taaatattca gttgaaatgc aaaatcccga cataactaaa cctgccggag ccggagacgt
    28561 tcctgcagct aactacaaga tgataggcgc tttggctgtt aaattaggtc aattggatag
    28621 aaaagattta gctgcatttg ctgataaaca tggtatgaac ggttgggctc caacacaagg
    28681 acacatacct tcaggtgttc catatttagg atttggaaga caggatatat tagacgggaa
    28741 gattaaaaat gctatgatag ttggaaaggg aagcttattc cttggaagaa tgactaactt
    28801 gtttgatggt gtttcctttg ttattgaagc aaataaagga aaacaagaac cacaatcaac
    28861 aatttctgag gaaaaagtga aagaacttat agctcaagca ttaaagggct tcgcttcaaa
    28921 tcttatggca gaataggagg gaagcaatgt ctgaaaaaca agtaaatcaa ataattggca
    28981 aagttttcaa ccaattggcc gataccctaa taagcggaga atatggtcaa aagccaaggg
    29041 tagcagttac tgcattagga agcgagcatg gagaggatga agtctacaaa gcagctgcag
    29101 gaaccctaaa atacggaatt gagccatcag ttatcggtac aaaggctgat gaaagagtca
    29161 aggcagtaac tgtagcctca gaagacgatg ctcacaaaaa gatggaagaa ttgctggatt
    29221 ccggcagtgt tgacgctgct gttacaatgc attatccgtt tccaatcggc gtttccacag
    29281 taggaagaat tgtaactcca ggcaagggaa aggaaatgtt tattgcaaca acaacaggaa
    29341 cttcttcgac agacaaggtt gaaggaatgg tgaagaatgc cgtctatgga ataattacag
    29401 caaaagcatg cggtataaaa aacccaacta tcgggatttt aaatgtagat ggtgnnnnnn
    29461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcaaaa gcatgcggta taaaaaaccc
    29581 aactatcggg attttaaatg tagatggtgc aagacaaact gaaattgcat taaacaagct
    29641 taaaaacaac ggatatgaaa taaatttcgc ccagtccgga agagcagacg ggggagctgt
    29701 tatgagaggc aatgacttgc ttacaggttc ctgtgatgtt atggttatgg attctcttac
    29761 cggcaatttg atgattaaaa tattttcttc ctatacaact ggaggaagct atgaatcact
    29821 gggttacgga tacggtcccg gcatcgggga aaactttgat aagctgatac ttattatttc
    29881 aagggcgtca ggagctccac tgattgaagg agcaatgaga tttgcaggag acttaataaa
    29941 aggaaactgc ctgcaagtgg ctaaagaaga atttggtaaa gccaaggcag ccggctttga
    30001 tgctattcta tcagaaataa aagcatccaa aaaacctgcg gctgaagaag cagaagttgc
    30061 cgctccgcca aaagaagttg taatttctca aatttcagga gtcgaaatca tggatcttga
    30121 agatgctgta aaattgttat ggaaaaacgg gatttatgct gaaagcggaa tgggatgcac
    30181 aggaccgatt attttggtaa gcgaagcaaa tacaaacaca gctcttaaag ttcttgcgga
    30241 caatgaattt atagcaaaag aacaattaga atgctgatgc taagtaaatc ctatttattt
    30301 caaaaaaatt cttcatccta ttattgatga agaattttta ttttaacttc attttatacc
    30361 gtcttattat aacatatata atttttattg aaaacttatg attttttgtt ataatggata
    30421 tgtgaggagt gatatcatga aaatgggatt tgaattaaac ctttctcaaa cccaaaagtt
    30481 aataatgaca ccagagttaa ggcaagccat tcaaatcttg cagtttaaca atgtggaact
    30541 gacggatttc ataaataaac aattggaagt caatcctttt ttggagtctg tcgataacaa
    30601 aagtgatgcc tcttcaaatg atgaaccggc agtggaaaac aaggaaagcg aagatataga
    30661 ctggcgggaa ctagcagaga aatatgacga tttcagctat aacacataca gctatgcaga
    30721 tggtgaaaac aagcagtcct ttgaaagcta tacctccaga aagctctccc tgaaagatca
    30781 cctgatggta cagcttggca tgagtgttaa aacaaacaaa gataagcgta tcggcgaatt
    30841 catcattgaa tcactggaca acaagggata tctgggatgc tcccttcagg atataagctt
    30901 actgataaat gaaaatattg ttgatgttga aaaagtttta aggctaatac aaacctttga
    30961 tcctgtagga gttgcttcca gggatttaag cgaatgtttg atgatacagc tgaaggaaaa
    31021 agggattcag gataaaaatg cttatattat tgcagaaaaa tatcttgaag atattgccac
    31081 caataaaatt caaaagatag cgaaagaact ccatatatct gtaagaagag tacagaacat
    31141 ttttgacata ataaaaatgc ttgagcccaa accttcaaga ggtttcatag tagacagcga
    31201 caatatcaga tatatcgtac ccgatgctac aatagaaaaa attaacgatg agtatataat
    31261 tattataaat gacagctatc tgcctacact gactattaac agatattaca aaaatatgat
    31321 aaatgagttg gaggataagg aagcaagcaa atttttaact gacaaactga attcatcgat
    31381 gtggcttata aagagtattg agcaaagaag aatgacttta tacaaagtga ttgaatctat
    31441 tttaaaattt caaaggaagt ttttcgatga aggaaagtcc gccttaaagc ctttagtatt
    31501 aaaggatgtt gctgatgaca taggtatgca cgaatcaacg gtaagcagag ctactaacgg
    31561 aaaatacgta cagacaccaa gaggtatcta tgaactgaaa tactttttcg caagcagtgt
    31621 aagcgaaact gacggaagca tggtatcatc cacaagcgtt aaatctataa tacaaaagtt
    31681 aatagaccag gaaagctcgc aaaaacctct gagtgatcaa aagataaccg aaatattaaa
    31741 tgaagagggt ataaatattt caagaagaac cgtggcaaag tacagagatg atttgagaat
    31801 accatcatct gccatgagaa gaagatatta agaaagaata tgatatataa tgcatattct
    31861 tttttttgtc aaaaaccgct acacaatatt taatttgtat gttataatca atttattaca
    31921 tagtgtccat atttaaacag cagatgttga aaataaggat aaaaaataag gataaaataa
    31981 taaagtataa tttaaattac aggaggatta tatggtaaga gtagcaatta acggatttgg
    32041 gagaattgga agattggcat taaggctgat ggctcaggat gaagaatttc aggttgtggc
    32101 aatcaacacc aattcagatg cggaaacatt ggcatatttg ctgaagtatg atactgcaca
    32161 cggtaattac atgacagata aaatttctta caaagacaac ataatgtatg ttaacggcaa
    32221 agaaataatc acatcaaggg taaaagaacc ggaaaattgc aattggaagg aactggacat
    32281 agacctggtt attgactgta ccggtaaatt caatgaaaaa gataaggcag tagctcacat
    32341 taaggcaggg gcaaaaaagg ttcttctttc agctcccggt aaaggggatt taaaaactgt
    32401 cgtttacaat gtaaatcata atattctgga tggaagtgaa acagttgtat ctgcagcatc
    32461 atgcacaaca aactgccttg ctcccctggc aaaagtttta aatgaagaat ttggaatagt
    32521 taaaggactt atgaccactg ttcacgccta tacaaatgac cagaacatcc atgacaactc
    32581 gcataaaagc ggtataagag caagaagagg acgtgcggca gcagctaata tagtacctgc
    32641 atcaacagga gcagctaaaa ctgtaggtgt tgtaatgcct gaattaaaag gaagacttga
    32701 cggcatagcc ctccgtgttc ctacactgac cgggtctgtt gtggatttga cagttgagct
    32761 tggaagaaat gttacagttg aagaagtaaa ccaggcagtt aagaatgcag caaatgaaac
    32821 cttgacatat actgaagatc ccatagtgtc aagcgatgtt atcggaagcc attacggttg
    32881 tatatttgac gccttatcca ctcagttgat agaagctgac ggcaaacagc tggttaaatt
    32941 aattgcctgg tatgacaatg aaatgtcctt taccgctcaa ttggtgagac tggcaaaata
    33001 ttggggctca atgaagtaaa agcaataaat cattaaatga agagggacac atatgtgtcc
    33061 cttatggata ttccggagga ttactatgaa taaaaaaact ttaaaggatt taaattttga
    33121 aaacaagaaa gtactggtga gatgtgattt taacgtaccc ttagacaaag aggggaacat
    33181 tacggatgat ataagaattg taagctctct tccaacaatt aattatcttt tagacaaggg
    33241 ggcgtctcta atattgatgt cacatctggg aaggcctgac ggagctcctg ataaaaaata
    33301 ttcgttaaag cctgtagctg aaagattaag cactctttta gagaaaaatg tcctttttga
    33361 ggacagcgat caggtagtag atgatacagt aaaagcaaac gcggcaaagc ttaaagtcgg
    33421 agaccttatg cttcttcaaa atacacgatt caggaaagaa gaaacaaaga acgcaggcac
    33481 ctttgcaaaa gacctggcat ccctggggga cttatttgtt aatgacgcat tcggaactgc
    33541 ccacagggct cattcctcaa atgcaggcat ttgtgaacat cttccttctg cccttggatt
    33601 actagtcgaa aaagaagtag agataatggg taatgcttta aaagaaccga aaagacctct
    33661 tacagccata ctgggaggag caaaggtatc ggataaaata gcagttatag aaaatttatt
    33721 aaatattgca gataatgttt taataggggg aggtatgatg tatactttcc ttaaggcaaa
    33781 aggatttaat acaggaagct ctttgctgga agaagataaa gttgaccttg ctaaaagtat
    33841 aattgaaaaa tctgcagaaa agctgttatt acccttggat actgttgttg caaaagaatt
    33901 taaaaatgat acagaatttt tcacaacaga tgctgataaa atacctgatg gttatatggg
    33961 ccttgatatc ggcagtaaga ctgtagaaat gtttacagaa attataaata attctaaaac
    34021 gataatttgg aatggtcctc ttggtgtttt tgaaatggac aactttgcca acggaactaa
    34081 ttcaatagca aaagcaattg cagacaataa ggacgcaata tcaatcatcg ggggaggcga
    34141 cagtgccgca gcagttgaaa agatagggct ggcagatatg attacccaca tttccacagg
    34201 aggcggcgct tcgcttgaat tcctggaagg aaaagtactg ccgggaattg aagcggtaga
    34261 caataaataa aataccctgc cggggatggt ttacagggac atacatggag ccccggatat
    34321 gaaaggaggc agaatgagaa gaaaattaat ggcaggcaac tggaagatga acaatgatgt
    34381 tgaagaaagc cttaaacttg taaaaagttt aaaagattca cccttgaatt cggatgttga
    34441 cgtattgatt tgccctccct ttacatcatt gtatcctgta attttggaac ttaaaaactc
    34501 ttcaataaaa gtcggagcac agaatatgca ctttgaagac agcggtgcct atacagggga
    34561 ggtatcacca acaattttaa aaaaccttgg tgttgaatat gtaataatag gtcattctga
    34621 aagaagacaa cattttggtg aaacagacca atttataaac aaaaaagtca agtcagcctt
    34681 aaatcacggt ctaaaaccga ttctttgcgt cggtgaatcc ttggaacaaa gagaagaagg
    34741 gattgagaaa gaaaccgtaa gaactcaatt aataatggat ttagagggca tcacagaagg
    34801 ggaaaagata gttgttgcct atgaacccat atgggcaata gggacaggta ggactgctac
    34861 aagtgctcaa gctaatgaaa tgtctgcctt tataagggct gttttaggag atattttcac
    34921 aaaagatact gctcaaaaca taataattca atacggaggc agcgtaaaag cttccaatgc
    34981 aaaagaaata atgcttcagt ccgatataga cggtgcattg gtcggcggtg ccagcttaaa
    35041 agctgatgaa tttataaaga ttataaattt tgaaaattga aaggttaaac tatgaataaa
    35101 gtaactgcat tgattatact tgacggatgg ggcattggac ccgactatga gggcaatgcc
    35161 attaaaagag caaaaacacc taattttgat gttttattga gacggtatcc aaatacaaca
    35221 ttatctgcca gcggctatga tgtaggcctt ccaaaagggc aaatgggaaa ttctgaggtg
    35281 gggcacttaa atataggggc aggtagaatt gtataccaag atttaaccag aatcaccatg
    35341 tcaattgaag acggggagtt tgaaaaaaat gaagccttgc tgggcgccat aaactatgca
    35401 aaagaaaaca ggtcttcctt acatttaata gggcttttat ctgatggagg cgtccacagc
    35461 cacaatactc atctgtacag tttgattgag cttgctgaaa aaaacggctt aagcgatgtt
    35521 caaatacact gcattacaga cgggagggat gtaagtccta caagcagtcc caattacata
    35581 aaggaattaa tggaaaaaac aaaaaaagcc ggtacagggc gaattgcatc cattatgggc
    35641 agatactatg ccatggacag aaacagacag tgggacaggg ttgaattagc ttacaatgct
    35701 ttgacaaaag gggaaggcga aatattcaag gaccctgttg aagctgtaag gaattcctac
    35761 ttaaccggta ttacagacga gtttatcaag ccaattctca tagaaaagga aaacggcgga
    35821 cttggaacaa tcaaagacaa tgatgcggtt attttcttta acttcagacc tgacagggca
    35881 agagaaatta cgagagcatt tgttgatgat gatttttcgt attttaacag ggaaaaatta
    35941 aatgttaagt atgtatgcat gaccatgtat gataagacta taaaaaatgt agaaattgca
    36001 tataaaccaa attttgtacg aaatacatta ggtgaatatt tgagcagcct gggcattaaa
    36061 cagttaagag ctgctgaaac tgaaaaatat gctcatgtca catatttttt aaacggcgaa
    36121 attgaggagc cctttgaaaa tgaggttaga attttaatac cttcccctga tgttccaact
    36181 tatgatttaa aacctgaaat gagcgcctat gagttgaaag acacagtaat ggagcagctg
    36241 gacaaggata tttatcaggt tatgataatt aattttgcca atcctgatat ggttggacat
    36301 acgggagata tagaagcagc tgtgaaagca gtggaaactg tagataaatg tatgggtgaa
    36361 attgtaaact ttataataag agctgaagga acggctataa ttacatcaga ccacggcaat
    36421 tgcgaggaaa tgtttgacaa agaaacactt gatacattta cagcccacac tgtcaacagg
    36481 gtgcctttta tagttgtaag caaaaagaac ttaaatttaa aagaaggaat acttgctgat
    36541 gtggctccaa caatgcttga tttgatgggt cttaaaaaac cacatgatat gacaggaaac
    36601 agtttaatag aaatataaag gagaaaaatt atgtctataa taacagatgt atatgcaaga
    36661 gaaatactgg attcaagagc gaatcctaca attgaagtag aagtatggac agaaagcggc
    36721 ggattcggca gggcaggcgt accctccgga gcttcaacag gagcatttga agccgtagag
    36781 cttagagacg gtgacaaaaa cagatattta ggcaaagggg tcttaactgc tgttgacaat
    36841 gttaacaata taattgcccc tgaattgata ggaagagatt cattaaacca gcttgaaata
    36901 gacaaactgt tgatagaact tgacggcaca ccaaataaag gaaaattggg agctaatgca
    36961 actttgggag tatccatggc atgtgcaaga gcagcagctg atacccttgg tcttcctctt
    37021 tatcagtatc ttggaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa
    37141 ggaagagtat ttacccagga agaaatggtt gaatactatg cagacctggt tgacaaatat
    37201 cccataatat caattgaaga cggtatgaat gaagaagact gggaagggtg gaaaatgctt
    37261 acggaaaaac ttggagacaa aattcagctt gtaggggatg acttgttcgt aaccaatacg
    37321 gtaaggttga aaaaaggaat tgaactgaag gttgccaact caatactgat aaaactaaat
    37381 cagattggaa caataacaga aacgctggat gcgatagaaa tggcgaaaag agcaggatac
    37441 actgctgttg tttcacaccg ttcgggagaa accgacgact ctacaattgc agacctggta
    37501 attgccgtta atgcaggaca gataaaaaca ggagctcctt cgagaatgga ccgtgtagca
    37561 aaatataacc agcttttaag actggaagac atacttggtg atactgccca atacaaggga
    37621 ctggatgttt tctacaatat aaaaggttag aataattatt attttatatt gcatatagcc
    37681 tgccggttat ttccgggaat catttcaggg acattctcct tactgaaaaa tttggaggaa
    37741 tgtccccata tatcctaaaa ctggaagggc atctttgttt tgaattgtta aaaaatatag
    37801 ttgatttaca aaaactttgg tgttataatt caaatgttat gcacagaaag caacaggagg
    37861 tgttttagtg gaaatagtcg taaggattct tttgataatt gcaagtttaa tattgatagc
    37921 aagtattttg cttcagtctg gcaaatctgc cggtatgtcc ggtgaaattg caggcggagc
    37981 tgaatcgtta tggggcaaga acaaaggaag aagctatgaa ggcaaactgg aaaaagctac
    38041 aaccgcagca gcgattatat tcgttattgc ttcattaatt ctagtggcaa ttcaatgata
    38101 gtttgtaatt ttcggtaata ttgttattct tatagtgtca aaatatattt taattttacg
    38161 ggcagatgat attatcatct gctaaattgt aaataatatg gaggaaggga ataaaaatgg
    38221 atactttaat tatagctccc gtagttggag ttttagcttt attatttgct ttctacaagg
    38281 catcggttgt gagtaaggct gatccgggaa ctgataggat gcaggagatt tcttcataca
    38341 tacacgaagg agcaatggca tttctggcaa gacaatacaa gtcagtaagt atttttgcaa
    38401 tagttatgtt tattatttta ggaattgcaa ttaatttgcc tacagcagca gcatttgcag
    38461 caggagctgc atgctcagcc tttgcaggat attttggaat gacggttgca acaaaagcca
    38521 acgtaagaac tacaaatgca gcaatgacgt caggaatggg aaaggcactt gatattgcat
    38581 tctccggcgg gtcagttatg ggtatgactg ttgttggact tggagtaata ggaacaggta
    38641 tattctggat gatttttaaa gatgcgactg tagttacagg attcagcctg ggtgcttcat
    38701 ccatagcatt atttgcccgt gtgggcggag gcatttatac caaggctgca gacgttggag
    38761 cagacctggt tggaaaggtt gaagcgggaa ttcctgaaga tgacccgaga aaccctgcgg
    38821 ttatagcaga caatgtaggt gacaacgtgg gtgacgttgc aggaatgggt gcagacttgt
    38881 ttgaatccta tgttggagca atagtttcag caattacatt aggcgttatt tcatttcaag
    38941 gcggtgacgg agttttattt gcattgctgg tatctgcagt gggtattata gcatccatat
    39001 tgggaacagc atttgtaagg ggagaatcaa atccgcagaa agcattaaac atgggcacat
    39061 acgcttcgtc agcaattgta attgcagcat catttttctt aagcaagtct atcttaggaa
    39121 gctaccagcc atttggagca attatattcg gtgtgtttgt aggattaatt attgcaaaga
    39181 taactgaaat ttacacctcc gatgaatata aatatgtaaa agaaatagca gctcaatctg
    39241 aaacaggagc agcaactaca attataagcg gtttgtctgt aggtatgaga tctaccgcat
    39301 ttccgacaat tgttttggca atcggtataa ttggagcata tgcaattgca ggttatactc
    39361 cggaaggtgt tgatgctgta agaggtttgt acggcatagc attggcagca gtagggatgc
    39421 tgtccacaac cggtatgaca atagcagttg atgcctacgg acctatatct gacaatgccg
    39481 gcggtattgc cgaaatgtgc gagctgcctc atgaagtaag agaaattaca gacagactgg
    39541 acgctgtggg gaatacaaca gctgcaatag gcaaaggatt tgcaatagga tcagcagctt
    39601 tgacggcact tgctttgttt gcttcatata ctcaggcagt aggaattaca tctataagct
    39661 taactgaacc aaaggtaata gccggtctat tcattggcgg aatgcttccg tttttattca
    39721 ctgcaattac aatgagtgct gtcggcaagg ctgcattctc aatgatagaa gaagtaagaa
    39781 gacaattcag agagatgcct ggagttatgg aaggtaccac aaagcctgat tataaaaaat
    39841 gcgttgatat ttcaactcag gctgcattaa aagaaatggt tgttccggga cttttggcag
    39901 tcatagttcc tttggcagtt ggatttttgc tgggaaaaga agctttaggc ggattacagg
    39961 caggtgcatt ggttacaggt gtacttatgg cagtattcat gtcaaattca ggaggagcat
    40021 gggacaatgc aaagaaatat attgaaggcg gcaaacatgg cggcaaagga agccatgccc
    40081 accaggctgc agtagttgga gacacggtag gagacccctt taaagacaca tcaggtcctt
    40141 caatcaatat attaataaaa ctaataactg ttgtatcatt aatatttggt tccttattag
    40201 gaaatggaat aatttaaata aaaatgaaat agcggcctct cttaagagag gtcttttttc
    40261 ttgcagatgt attattttgg tgatataatc atttcaatta attgacatat aattatatgg
    40321 ggggttagga tgtatattga tattgacggt ctaaatatca actacacaga tgagggtaac
    40381 gggaaaagtg ttttgctcct ccatggatgg ggcggcagca ttcaaaccat gatggcgatt
    40441 ggaaatatat taaaagataa attcagagtc atatcagtgg atctgccggg attcggaaaa
    40501 agcggcattc ctgaagttcc gtgggattcc tatgattatg ccgaatgcat atataaattc
    40561 atccaaaagt taaatttaag cggactgata gtattcggcc attctcacgg aggcaggatt
    40621 tcgataatat tagcaagcaa atacgaagtt gtaagaaaac tgatacttat tgacagtgca
    40681 ggaataattc ctaaaaggaa ggctaaatat tacataaaag tatactggtt taaaatgctt
    40741 aaaaagattt acaaggctct acctgcaaag aataaaaaag aaaaaatgga taaattctat
    40801 aaaaaattcg gcgctcaaga ttacagagat tccgacggag ttatgcgcca aacaatggtc
    40861 aaggtaataa atgataattt atttggcctt ttaaggctta ttaaagcgcc caccttgttg
    40921 atttggggag aaaatgatga agacacccct gtttatatgg gcaggttaat ggaaaaagaa
    40981 attcctgaca gtggtctggt tttattgaaa ggggccgggc attattcata cgttgataat
    41041 tatgaccagt tcagagcagt aataaatgta ttcttaaaag aggatttagg agagtgaaaa
    41101 tgcagattat agtaagtcta tatttattat tttggtttta tttatgcgga cttaaaataa
    41161 gacaaagcct tcatataatg cagctggaag gatacgacag cgataagttc ctaaactgga
    41221 ttgaaaataa caaagatagg tttcatacaa gaaatgataa aatatataca attgttttaa
    41281 taattatttc ggtaatccta gcagttactt ctcctataga gaaaataatt atctatcctt
    41341 tgatatatgc cggaatatca atattcttca tgattaaaaa taaccgtgaa aaggaagaat
    41401 caaaaaaacc cctggttttc acaccaagag ctaaacgatt gtacggtgcg gcatattttc
    41461 tgcttgcatt tgacctggta ttgactatat taattgtcag atggattaca aaggatatat
    41521 tgtcctatct tcctgtatgg gcagggattc tgaccgtgat ttactatttt tcatcattgt
    41581 acatttacgg ggcaaatttc atatcaaagc ccgttgagga aagaataaat aaaagatact
    41641 atattcaagc ttccgaaaaa attaaaaaga tgaacattta ttctataggc ataacaggca
    41701 gctacggaaa aacaagcact aaatacattt ccgaaaccat attaagggaa aagtataaag
    41761 ttttaaaaac accggaaagc tacaatacac ctatgggaat aagcaaaata ataaacaatg
    41821 aattaagcgg cgaatatgat gttttcattg ccgagcttgg tgcaagcaaa acaggagaca
    41881 tagacgaagt agcaaaactt acaaacccaa aactgggcat tataacttca ataggaccct
    41941 gccacctgga aacgtttaag tctattgaca atataatgag aaccaaatat gaacttattg
    42001 aaaatcttcc tgatgatgga atagcaattt ttaattacga caatgaattt gtaaaaaaac
    42061 ttgctgataa aactttcaag gagaaagtac tctacggaat tgacaatatt gaagaaacag
    42121 atgtatttgc aaccggcatt cacgttgaca gccttggctc gtcatttacc ttatgcatca
    42181 agagcctggg gacaatagaa tgcaaaacaa aacttttggg aaggcacaat atcctgaata
    42241 ttttggcagg tgcagcagct gcatctattt taggattgtc tctggatgaa ataaaaagag
    42301 gaatatctaa gctggaaccg gttgagcaca ggcttcagct catagaccag ggcacaggtg
    42361 tagtggtcat agatgatgca tttaattcaa acccggacgg agcaaaggct gccttggatg
    42421 tactgaattc ttttacagac aggagaaaga taatagttac gcccggaatg attgaattag
    42481 gcagcataga agaaagtgaa aatttcaaat tgggagaata tattgcagtg gtttgtgata
    42541 ttgcaatatt agtggggaaa aagcggaccc tgcccatata ccaaggcatg aaaaagcata
    42601 atttcaatga agataatttg tatgtggtta attcattgaa tgaagctaca gaagtattga
    42661 aaactatcac aaaagtcaat gacgcggttc tttttgaaaa tgatctgccc gacacatacg
    42721 acgaaaaata atatggaggg aagccatgaa aaaaataggt attatcatcg gcggaaaatc
    42781 cgtcgaacat gaagtttcta taattacagg tatgcaggtt tttgaaaaca tagacagaac
    42841 tatttatgaa cccaggataa tctatataca aaaggacggc aagtggctgg ttggcgaaag
    42901 tctccatgac ataaacaatt ttaaggctaa aaagctggaa gatgcatatg aagttttacc
    42961 gggatttaaa gaggggaaac tgattcttta tcctcatcct gaacttaaaa ccggtatttt
    43021 cgggaaaaag tactctgtat atgaaattga tgttgtattt cccgctgttc acggcacaaa
    43081 tgtggaagac ggttctctgc agggcttgtt tcaaatgaac ggggttcctt atgcctttgg
    43141 ctccgtatta tgctctgcag tgggaatgga taagattata atgaagaaag tatttcaaag
    43201 ctatggcctg cctgttgtgg attatacctg gttttacaga agcagttttg tagaaaacaa
    43261 ggatgaaatc atcaagcggg cagagaatat aggatatccc ctgattgtaa agcctgccaa
    43321 tttaggttca agcgtcggta taaacaaggc aaaaaatttg gatgaactat tatttgctat
    43381 tgatgttgcc atgtcctatg acagaaaaat aatactggaa aaatgcatcg aaaatgtgcg
    43441 tgaaattaac tgcgccgttc tagggtacga agacgagctt gaaacttccc tttgcgaaga
    43501 gccggtggga tggaaagaat tcctgaccta tgaagataaa tatgtgaata aaaagaagga
    43561 ttcaagggaa tcaaaaaaaa gaatacctgc tgaaattgat gaagaaattg aaaatcaaat
    43621 aaagtattat gctatggaag ccttcaaggc agtggacggc tgcggaaatg caagaataga
    43681 ttttctttta gatggcaaca atatatttgt aaatgaaatt aacactattc caggatcaat
    43741 ttccttttat ctttgggaag gaaagggaat atcatttaaa cagttaatca caagaataat
    43801 acagcttgca gaaattcacg gcagccaaag aaaggtcaat attgtttcct atgatattga
    43861 tttgttaaac aagatgggcg ggggcggaaa aaagaaataa ctcaggagaa atacatgaat
    43921 tttaaagata aaatactggc atttatgaag gatgaagcat ataagccctt aacattcaaa
    43981 gaacttcttc aagcatttga agttgaaggt aagatgaaaa aagcactttt aacagctttg
    44041 aacgaactgg aagatgaagg gaaaataata tttacccggt ctgaaagata cggcctgccg
    44101 gaaaaaatga atctggttac gggcaccctg gagggaaatc aaaaaggata tgcctttttg
    44161 atacccgatg acaagaactt aaaggacctc ttcatttctc cggtggacat gaacagtgca
    44221 atgcacggag acagggtaat cgttagacct atgaaggtct tggaagatgt caaaagccct
    44281 gaaggaaaag ttataagaat aatagagaga gccaatcaat atgtaatagg aacatttcaa
    44341 aaaagcaggc actttggctt tgtggtgcct gatgacaaaa gaattgcctt tgatatattt
    44401 gtcccaagag aagaatttaa caatgcagaa gaaaacaaca aggtttccgt aaaaattacc
    44461 gagtggcctg accagagaaa gaaccctgaa ggcacaatag ttgaggtaat aggggacagt
    44521 gaagatacca agacccatat tgatgcggtc ctcctggcta agaaggtaag gcaggtattc
    44581 cccgaggatg taataaagga agcaaaaaga gtatctgaag aaggaataca tgaattagaa
    44641 ctgaaacgca gaaaagactt aagaagcctt gatataatta ccattgacgg cagcgatgca
    44701 aaggatttgg atgatgcggt ttatgcaaaa aagctgaatg acagtgaata cttcttaggt
    44761 gtgcatattg ccgatgtaac tcattatgtg aaggaagaca ggaagcttga caaagaagcc
    44821 cttaaaaggg gaaccagcat atatctgaca gaccgggtca tcccaatgct tccaaaggaa
    44881 ctgtcaaacg gagtatgcag tttgaatccc ggggaggata agcttacact ttccgttgag
    44941 atgattataa ataatgaagg caaagtaata gactataaga tatttgaaag cataatgaac
    45001 aaccgttaca gaatgaccta tgaagatgtg acggatattc ttgaaaacaa taatgaagaa
    45061 ctgattgaaa aatataaaga catagttccc atgcttaaaa caatggaaga attaagcctg
    45121 atactgagaa gaaaaaggga cctgagaggg gccattgatt ttgaatttcc agaaagaaaa
    45181 atcataaccg atgaagaggg cagggcaatt gatgttacaa agtatgaaag aagaacttca
    45241 aacaagataa tcgaagaatt tatgcttgta tgtaatgaaa cagttgcaga gcattattac
    45301 tggctaaaca tgccctttgt ttacagagtc cacgaggacc cggacgaaga aaaaatgtat
    45361 gaattcagca aattcattca taacttcggc tacacactaa aaggcaatga tattcatcca
    45421 aaggagctgc agcagctttt aaacaaaatt aaaaatacta aagaagaatc cctgattaat
    45481 accatgatgc ttaggtcctt gaaaaaagca atatattcac ctgattcatc aactcacttc
    45541 ggtctggctg caaattatta ctgtcacttt acatctccca taagaagata tccggacctg
    45601 cagatacaca ggattatcaa gggccagata aacggaagat actctgaaca agctctccaa
    45661 aagctatttg aaagaacggc ggtggttgct gaacagtctt caaaaatgga aagaattgca
    45721 gacgaggttg aaagagatac tgaaaaaatc aaaattgctg aatttatgtc tgataaaatc
    45781 ggtgaggaat atgagggtgt tgtttcaggg gtaaccagct tcggaatttt cgtggagctt
    45841 gaaaacacag tagaaggact tgttcatatt tcaaacatgg ttgatgatta ctatatttat
    45901 gatgacgaga aaaaggaact tttcggtcag ggcagccgta aggtatttaa aatcggcaac
    45961 gctgttaaaa tcagagtaga taaggtcagt atagcaaaag cagaaataga ttttgcatta
    46021 atttaaaata tgtgatagaa taggtttaga aattaagcag ataatattat taagttttgt
    46081 ttaagcttaa tcttctaaga ttgttaaaga agtaatcatt aactgacccc tttatttcgg
    46141 gggcgtaaat ggtttcgacg gggatataga agtaagataa gcgagtcgtt gcgccaaaca
    46201 acgttaaaag cggcaaaaac aataaacgca aacgaaaata atttagctta cgcagcttaa
    46261 ttgctgcagc ttgttccgct taaccggcct gtaggttaaa ccggggcatc atttaaatca
    46321 gggaactgct tgaggggctc ttcgacctta agctgaacaa tcggagatag caaagattac
    46381 agcttggcca taggcggtaa tcggagcgaa actcaaatta tggtctgcac tcggagaaag
    46441 tcttgtggaa atattttcgg acgtgggttc gactcccacc gcctccatta atgtaatacg
    46501 atttattgca ttttagtatg ggaaattagc aaattagcaa aagctatatt cataatgatt
    46561 atgaatatag cttttttgtt ttgttcaata tcataaaata tgaaatgagt gcaaagtcaa
    46621 tacgatgtgc tcgagaaggc aagtaacaga atagcttatg agaccacatt aaaactcttc
    46681 aaatctgagg agttttatta attaaattta acagtaaagg ttctgatttt atggtataat
    46741 aagaaaaagg aatattcata tacataaatt gtaaaaaaaa cagaaaagga agtgattata
    46801 taaatacatt attattttct gtggtaaaca gaggtaaggc caatgaggtg ctcagcaaag
    46861 caaaggaatg cggcgcaaca ggagggacaa tttttttggg ggaaggcaca ttacaatcca
    46921 ataaactatt agatttacta ggtctaaata aagtccatag agaaatcctt atgattgaag
    46981 ctacagagga aatatgcagc gacttgcagg aaaaactcag cgaccacttt aggttctcca
    47041 aaaaaaataa aggaattgca ttctctgtac cttttatccg ctggaaatta aaatcaaatg
    47101 aagaggaaat aagagaatta cacagaaatt tcaatccttc gcatttttgc ataatgacaa
    47161 tcatagataa aggtaaaagc aacgactgca ttaaagccgc aaaggctgca ggagcaagag
    47221 gcggaactgt aatgcacgga cacggggcag gtgttcccac ggattattat tttccactga
    47281 ttattgaacc gcaaaaggat gttgtaatgg ttatagccgg aaaagataat gttaatgcaa
    47341 taaaagacag gataataaat gagatggagc tttttaaaga aggaaagggt attattttta
    47401 ttcttccggt tgtaaaaata agcggatttt cagataacca ggaggtaaag gcatgaatct
    47461 gctgtttgaa aaaacgaaag aagttgctca aaccttgcta ccggttgtta ttctagtttt
    47521 gcttattagt ttcaccatgg tggatgttgg agctaatgtt ataatgcggt ttatagcagg
    47581 aagtattatg cttttaatag gactttcaat ttttttgctg ggtgtagatt tatccatgaa
    47641 ttctattgga gagcatatgt caatggaagt ggctacttca aggtcatctc ttaaaattac
    47701 aatacttagt tttattctgg gttttttgat taccgttgca gaaccggatt tattaatatt
    47761 aggatcccag actgaaattg cttcaggagg atcactcgtt tcatcaataa ttgtatacat
    47821 ggtatccata ggcgtaggag ttttaatttc acttggagtt tttagattat taaaggatat
    47881 aactcttaat ttatttatgg caattacata tgctgttata tttgtattag cattatttgt
    47941 atccgaagaa tttctagcca tttcctttga ttcttccggt gcaactaccg gagctcttac
    48001 aacacctttt gtattgtcct tgtcattagg gctgtctcaa attaaaggcg gcatgaaatc
    48061 tgaagagaac tcctttgggc tggttggcat tatgagtgcg ggacccatat tggcagttat
    48121 gctcttatca ataatttcag ggcagagaaa tatacatggc gaagctgctg aatttgttgt
    48181 ggcagaaggt gtacttggtc ctataataaa aataattccc gcaatttttg ttgaatcatt
    48241 aactgcattg ttgcctataa cagctctatt tttcatttat aactttgcaa aatttaagat
    48301 agataaggat gaacttttta aaattataag agggcttgta ttggtgctgc ttggattgac
    48361 attatttctt acagctgtta actccggatt tatggatatg ggcaggatat taggtatgga
    48421 aatcgctaga atgaatcctt ggatattgat aggcataggc ttcgtattgg gcttcatagt
    48481 tgttctcgtt gaacctgcag tacatgtttt aggtgagcaa attgaagaag ttaccggcgg
    48541 ccatatacct ttgaacttaa tcagaatgac tctttcaatc ggtgttgcat tggcaatttc
    48601 tctttccatg gtaaggattt tgattcctga ggtaaaacta tggtattttc ttctacccgg
    48661 atttgctatt gcaattttgc tgtcatttat agcagaacct gtctttgtgg gaattgcata
    48721 tgatgcagga ggagttgctt caggtcctat gacagcaacc tttgtgcttg cttttgccca
    48781 aggggcggca tcctccatag aaacagcaaa tgtgctggtg gatggtttcg gtgttatagc
    48841 aatggtagcc atggctcctg tattttccat catgatattg ggtactgttt tcagatataa
    48901 gaaggttgaa gaatatactc ctgttgaaga agaatacatt atttctcctg aaatacctgt
    48961 agaagaaagt ctggtattcg actgtattat ggtaaatgtt gaaagaggtt ttgctgaaag
    49021 tgtagttgag ctggctcgta aatcaggtgc agcaggagct actataatgc acggaagagg
    49081 aactgataag catcaaaaag ttaagctgcc gattatgaat atagagttgc agcctgaaaa
    49141 agaaattgtt atattcatta ccagctcaaa tttcactgag aaggtagccg acaatttgtt
    49201 aagaaatgat aagcttaggg aagaagggga tatggttatt catatttgtc ctgctacaga
    49261 ttctatgata aagtatgctt ctatcacaaa tggtgaaaaa tgaaagcata aaaattaata
    49321 aactcctcga actttgaggt gtttttttct aaaatcatgg tataattact acatatagct
    49381 ttcaaatgaa ttatcaggga gaaacaatga ataactttaa gaagttttta tatgaaaccc
    49441 tgtatttaac taccggctgt ttcataactg ctttttctgt aaattatatt ctgaagccaa
    49501 acgggcttat taccagcggt ataacaggat tatccataat acttgaaaga tatctgttaa
    49561 ttgactatta ctacatatat tacatggcta ctttaataat tttagcttcc actttactat
    49621 taatgggtaa aaaagaactg atgaggatta tttttctttc tgttttgtat cctacagtat
    49681 tgctgttaat gcagggtgtt gaatttaaat ttgtggaaaa tgaccttttg cttgcttcaa
    49741 tatacttttc actattttac ggtatagggg tcggcatgat actccgaaaa ggtttctcct
    49801 tcggaggaac cgatactatt gcaagaatac tcaacaagaa gctttttccc tcaataaatg
    49861 taagctattt gatgcttgtt ttggatggtt ttgttataat agctgcagcc tttacctttg
    49921 gaagaaacat tgcactgtat tcaattatta gtcagttgat atttaccaag gtttgcgatt
    49981 atattatgtt cggcttcggc acaaaactgt acaagcttga aataatcagc agtcaatttg
    50041 aagaaataag caattatata atgcatgagc ttggaagagg tgtaactctc tttaaaatta
    50101 agggatgcta caccaatgag gaaaaagttc agatagaatc aatatgctct cccaatgaag
    50161 cagttatggt acaaagactt ataaagaaaa ttgagccggg agcatttgta aaagtattgc
    50221 ctgtagttaa tgtctggggc aagggcagac gctttataga tatcagcaat gaagattgaa
    50281 atattatgga gggaatatga ataattcagg agacctgccg gaatatgtaa cggttgtagg
    50341 cggcgtaaat gttgatattc aaggatttac taaacataaa ctgatacccg gagattcaaa
    50401 tatcggaagg gttaaaatct ctttaggcgg agtaggaaga aacataggtg aaaacctgat
    50461 acgcctgggg ataaatacta aactgataag tgttgtggga gatgatgtat acagcagtaa
    50521 aattttaaat gattccagag aaacaggttt ggatataagt gacagcatgg cagtaaaatg
    50581 tgaaaatgcc tcaatttacc tggctgtatt ggatgaagac aatgacttgc atatagcaat
    50641 aaacagtatg gatatcatgg aaaaaatgac tgttgatttt attaaagaaa aaaagcatat
    50701 ttttgataag accagacttt tagttatgga taccaatatt cctcaagatg taatcgaata
    50761 tttactgcat gaatacaaga ggaaagatat ttttcttgat accgtttctg tgtccaaggc
    50821 aaaaaaaatc aaaaatcttg taggacatat tcacacactg aaaacaaaca aacttgaagt
    50881 tcaagcattg acagaaatag aagtcacaga tgaggatagc ctggtaaaaa acggccaata
    50941 cttactaaac aggggagtta aacaaatctt tattaccttg ggcaaaaatg gtgtgtttta
    51001 cttgaataaa agtacaaagc gaattataag agctgataat atcaagcctg ttaatgcaac
    51061 aggcgcaggc gatgcattta tgtcagccct gatttactgc cacctgaagg gtatcgatat
    51121 taacgaggct gcaaagtttg caacggctgc ttcaataata gcactttcac atgaaaatac
    51181 aatcaatcca aatatgtcaa aagaaaatat taatataaaa gagaaggagt taaaatatta
    51241 tgaaaaatta tttgaatgtt aatccggaag taaaagaagc attagagaag ggcaaagcag
    51301 ttgtggccct ggaatcaaca attatatcac acggcatgcc atatcctaaa aatgtggaaa
    51361 cagcattaaa tgtagaaaaa atcattcgcg ataacggtgc tgttcctgcc acaatagcaa
    51421 tacttggagg agtgttaaag gtcgggctta ctgaggaaga aattgaatat ttgggaaaag
    51481 cagaaaatgt tttaaaaaca tccagaagag atattccgtt tatagtatca aaaaaattag
    51541 acggtgctac tactgttgct tcaacaatga ttattgccgc catggcggga atcagggtct
    51601 ttgcaaccgg gggcattggc ggtgttcaca ggggagcaac caaaaccttt gatatatctg
    51661 cagatttgga ggaattatca aatacggatg ttgctgttgt ctgtgcagga gcaaaatcaa
    51721 ttctcgacat tggcttgacc atggaatatc ttgaaactca cggagtgcct gtagtcggat
    51781 ataaaaccgt ggaaatgcct gcattttata ctagaaaaag cggtttcaag gtagattaca
    51841 ctgttgacag ccctgtagaa atagcagaag ccttaaaagc aaaatgggac ctgggtctca
    51901 agggaggttt ggttgtagct aatccaatag aggaacaata tcaaatggat tatgatacga
    51961 taacagctgc aattgacaag gccgtttcag aagcagaaga aaccggtata aagggaaaag
    52021 aatcaacacc gtttttatta tccaaggtaa aggaaattac cgggggagac agtttagaat
    52081 caaatataat gcttgtttac aacaatgctc tgctgggagc aaaaattgct gtagagcttg
    52141 aaaaaatatc ataaaaacat tttaattagt ccatggttat gctatacttg ctttggttat
    52201 tatataggta ggaggatatt atgggtaagg atgtaattgt tgcttgtgat ttcggtaata
    52261 aagaagaagt taaatatttt ctttcaaagt tcacagaaga aaagccttat gtaaagattg
    52321 gcatggaatt attctacaga gagggtcctg agattataaa actcatcagt tcaatgggac
    52381 ataaaatatt tttagacctt aagcttcatg atattccaaa tacggttaaa aaaaccatga
    52441 gggttttatc agggcaaaac atacaaatgg taaacctgca tgcggcagga agcattccaa
    52501 tgatggaagc agctttagaa ggattaacac gggaggatgg ttcaagacct ttattaattg
    52561 ctgtaactca gcttacatct atcgatgaag aaacaatgaa aagagatatt ttaattgata
    52621 agcccctgga agaagtggta atacattatg caagatgtgc aaaaaaagca ggattggacg
    52681 gtgtcgtatg ctcgcccctt gaagcagaaa gggttcatac tgcctgcggg atagaatttt
    52741 taaccgtgac accgggaata aggtttgaag gaaacgaacc tggagaccaa aaaagagtta
    52801 caacaccggc aatggcaaaa atttcaggct ctgattacat agttgttgga agacctgtta
    52861 ccgaggctaa agaccctgta gaagcataca gaagatgttt aagagagttt acagtatagg
    52921 tggtatcatg gcggataaga ttgttgcaaa aaacaaacag gcatatcatg aatattttat
    52981 tgaggaaaaa tatgaagcag gcttggttct tgcaggaaca gaggttaaat ccataagaca
    53041 gggcaaggtt aatttaaagg acagctatgt tagcataaaa aatggtgaag cattcgttta
    53101 taacatgcac ataagcccat acgaaaaagg aaacatatac aatacagacc ctttaagaat
    53161 aagaaaactt ctattaaaca aaagggagat aagaaaacta aacggtatga ctactttgaa
    53221 gggatattcg cttataccct taagcctgta tcttaaaaac ggtctggtaa aaatggaact
    53281 cgccctggct aagggaaaga agctctatga caaacgccag gatcttgcaa aaaaagatgc
    53341 tgacagaagg gtgctgagac aagatgattt cagataaaca aattattgaa tcaggagaaa
    53401 agaatgaaaa cactttatga aggaaagact aaaaatttat tgaagaatga aaaagacggt
    53461 tttgtgtacc ttttgttcaa agacgacgca acaggagaaa acggagtctt tgacccgggt
    53521 tccaataccg tagggggaag cgttgaaggc aaaggaaagc tgggccttat aatttcaagt
    53581 tacttttttg agctcatgga aaaaaacggc ataccaaccc actatcttgg agctgatatt
    53641 gataagggct tgatgaaggt aagaagcctg actgttccga aactggaatt tgtgcttcgt
    53701 tactttaccg caggaagtat gtgcagaagg ttcagtatgg aggaaggaat accctttgac
    53761 cctccctata cggaagtaac ccttaaggat gatgaacagg gggatccctt aatcagtgaa
    53821 aggctatgca tgatgaaagg ccttttaaag gaaggacagc atgatgaagc catgaatgta
    53881 ttagtgaagg ttggaaaagt tttaaaggaa gagcttaaac aaatgaacct tacactaatt
    53941 gactttaaaa tagaaatagg atacgatgaa aacggaaaaa tattcgttgt tgacgaaata
    54001 actccggata tttggcgagt tcgcgacaag aacggaacaa ttcccaacca gattgaatgc
    54061 ggcaaaattg tactggaaaa aattcgtaat gcagtctgat aatatatttg tggaggtatt
    54121 aatgctggct gaacttataa aaaatggctt cggagaaaag gaagacttaa attgtgctga
    54181 aaaaatttta tggggagcta ataatgcata taatttaggt cttgaccgag aagcattgaa
    54241 attagcttcc ggttttgggg gaggaatggc aatagaagat gcctgcggtg ccctgaccgg
    54301 tgcaataatg gttttaggga agctttttgt cgttgaaaga gctcatgaaa gtgataagat
    54361 caaagagctg acaagagagt tatttgatga gtacaacaag gaaatgaaat ctattgactg
    54421 caagcccttg aaggatatgt acagaacaga agagttgaaa tgcaggaatg taatattaaa
    54481 ggcagcagaa atattggaca tgattgtttt gagagagctt aataaacccg cttcatagtg
    54541 aagcgggttt ttaaatatct gtgacttggt cacggatttt taaactatgc ttttttacaa
    54601 ttaatggaga aaatcataaa cttatgttag cttttatttt tgtcatcagg aagataattc
    54661 atctttacat agctcattaa ttgttgtata atttttaatg ttatgaaaaa atgttctaga
    54721 catatagtag gcaataggag gaaaaatgaa aaaagtatta acgattgttt tggtatttgc
    54781 attatctttt gggtttttaa cacttgttaa taatatgggt ggcaaaaaca ctgcatcagc
    54841 gcaagaaaca ctgacaggca aagctgaggg ttttggcggc gaaattaatg taacattaat
    54901 agtaaacggt gatgatatag tatcagtcga agcaacggga gatgatgaaa ctgaaggaat
    54961 aggctcttta gcaattgacg aggttcctgc tttgattcaa gaagctgatt cagcagatgt
    55021 tgaaggtgtt tcaggggcta cctatacaag cgacggtata aaagctgcag taagaaatgc
    55081 attagcatca aagggcggta gttcaagcga acaaacattg acaggaactg ctgaaggatt
    55141 cggcggagat attaatgtaa cggttgttgt aaaaggcaat gatattattt ctgttgaagc
    55201 aacaggcgat gatgaaactg agggcatcgg ctctttagca attgatgagc ttcctgcttt
    55261 gattcaagaa gctgattcag cagatattga aggtgtttca ggagctacct acacaagtga
    55321 cggcataaag gctgcagtaa aaagtgcatt ggaatccaag caataacaag catagagaac
    55381 aagaagctgt acatattgat acagcttctt ttatgttggc aagttgtaaa atgaattgcc
    55441 caaaaaaata aaaatagatc cattcctgga tcaaatcagt tatacttatg ttgaagacaa
    55501 aagtgactgg agtgaaacgg aatggatcaa aacaataata acatacaaaa aagaaaatgg
    55561 aaacaaatat ctgaaaaaga gcgttataaa attgaagcac tttatgagca aggactcaaa
    55621 gcaaagcaaa taggcgaatg tataaatcca atgcgtgata gacgaacaat agaaagggaa
    55681 ctgcaattag ggatagtaga acaaaggcgt ataaatccaa gctttaataa ataccaacca
    55741 ttatacatca cagaaaaggt ttataaagct gatacagcac agatgcgcca taatgagcgg
    55801 gcatcaaata aaggtcgtgg acttaaaatc ggacatgatc aggaattagc agattacatc
    55861 gagaataaaa ttaaagatga aaagtggtct ccggatgttg taatcggtag gataaaggca
    55921 gaaaacattc catttaagac aactaagtaa aattgccaaa aaactgtcaa aaatttttct
    55981 ccccctaggg ggagaaaccc caatagcaac acaagtacaa ctgataaaaa agttgggcaa
    56041 ttaaacttgc aatttaccac aatcctaaaa aatgaactta tttgattgac tttgaattta
    56101 tatattggta taatatatta tatatattaa tataatatat taagtattca aaaatttaaa
    56161 gatgaaaaag aattaatatt taaagtaata tacgaaaaaa catcccgaga attaggaaat
    56221 ttatgaaata aagtaatcat ttataggaaa acatttgccg aattaataat tttaaaaaag
    56281 tttttttggc atgaattttg cttaccatta agtagtttgc cggctaaaaa attttaggaa
    56341 tattaaggag gacaattatg ggtgaaatga aaaaaacatc aagagaagtt tctcttgaac
    56401 gtgcagaagt attgaaaagc aggatagatc catatttgga agctaaaaaa gaaattcctc
    56461 ttggtttaga ttttcaagat gagatcaaag aaagacaaaa gaaaataaaa gaatatttta
    56521 atgctacgga tgaagaatgg aatgactggc attggcaaat cagcaacagg atacatgatg
    56581 cagagacatt aaatcagttt atccatttaa cagacaaaca aaaagctgat attgataaag
    56641 ttggagaaaa attccgctgg gctatatccc cgtatttcct ttcagttata aacccaaaag
    56701 gaaatcattt tgaaaacccg atttacttac aatcaattcc aacaggttta gagcttgtag
    56761 aaggaatcgg tgaagccgac cctatgggag aagaatttac aaatccggct ccatgtatta
    56821 caagaagata tccggacaga ttggtaatca atgcaacaaa tgtgtgtgga atgtattgcc
    56881 gccactgtca aagaagaaga aatataggag aatttgatac tgctcaacca agagaaaatt
    56941 tacaagcagc agttgattac attgcagcta ataaagaaat cagagatgta ctggtgacag
    57001 gaggagaccc ctttactctg tcagatgata ctttagattg gttattgaca caattagaca
    57061 atattcctca tcttgaattc aaacgtattg gtacaagaat gccggtaaca ttgccgcaaa
    57121 gagtaacaaa agaaatgtgt gatatgttga aaaaacatca tcctttattt attaacttac
    57181 aatttaataa tccattagaa ataaccgaag aaacgaaaaa agcttgttca atgcttgctg
    57241 atgcaggaat accccttggc aatcaatcag tgttattaag aggtgttaat gatgataaat
    57301 ttgtgatgaa gagattaaat caaggtttat tatcttgcag agtcagacct tattatattt
    57361 tccatgcgaa aggtgtagta gggacactgc atttcaggac aagtgtagat attggaatag
    57421 aaataatgga gcatttaaga ggatatacat ccggcttagc aaatcctaca tttataatca
    57481 atgcaccaaa aggaaaagga aagactccaa tgcttccgga atacgttatt tcccacggca
    57541 gagatacaat aacaataaga acatgggaag gcacgataat agagtatcct aataaagatg
    57601 ccaatgtaaa atatgaatag acccggtaaa gtgcatttca ttaaacttta ataagctcct
    57661 gcgcaatttt ttgcgataaa tagatatatt ttgaaaatca taatttgctt ctgttatgaa
    57721 taataataat ggattttcat atcttaggag attaaaaagt aacaaagaat ttaaattgga
    57781 gggaaaaaaa tgaagaaact tttatcacta atggttgtat gtattttagt tctttctctt
    57841 gctgcttgcg gtgagcaaca gactccgcct cctgccagca acgacacaga taaacctgct
    57901 gaagaaaccc cagaaacccc tgaagtaacc agagaccctg tagaccttgt tatgggtaca
    57961 ggttccagcg gtggtactta ttttgcacta ggtggtgcaa tgtctaatgc ttggaacagc
    58021 tttttgaaag atgagcaaat cactgttaca gctcaggctt ccggtgcttc cgtagagaac
    58081 attaacctga ttaatgcagg tgaaatggat cttggtatgg ctatgaataa cgttgctgca
    58141 aacgcttttg ccggcacagg tgcgtttaac gctcccgtta cgaacgtatc ttccatagga
    58201 gttgtttaca acgaggtgta tcaaatcgtt gccaatgcca aaacaggtgc aaagaatgtt
    58261 gaagacctta agggtttgaa aatggccgtt gggcctgctg gctccggtac tgttggatta
    58321 actcagaagg ttttagctgc tgccggctta gacattgaca aggacataaa acgccaaagc
    58381 gatagtttcg gtgatgcggc tacgaagatg caggatggtc acattgatgc tgctgccaac
    58441 gtacttgctg ttccggcttc ttccatcatt gagatggcta ccgttatgga tcttgcctat
    58501 gtagatatca gtgatgaaat tcttgcaact attcaggctg atgaaccgta ttttgttcgt
    58561 aaggttattc ctgccggcac ttataaggat caggcagagg acatcaacac catcacttgt
    58621 aaagctgcat tgtattgccg tgctgacctt gatgaagaaa ccgtttatca aattaccaaa
    58681 gctatgtatg aaaccaatga tgtagttgct gcagcacata ctaccggtaa ggaaattcag
    58741 cttgagggtg ctcttgaagg tataacaact cctatacatg cgggtgctgc aagatattac
    58801 caagaaatgg gcatcgaagt tcctgacaac aaataaacag aaaaatatct atggggttta
    58861 atttgaataa tttgggtcaa gagcggacca ttaccatagg agggttcgct ctcttctact
    58921 taacattgtg tggaagaagt tcaataacca tgaaggaggt attacattta tgagtaaaga
    58981 caaaaatgaa aaacatattc cccctgttga tgaagataac atcgataaag ttaatgaaga
    59041 aaatatcgtt ttcaatgaaa acaacaaaac agtcgataaa gaaagcattg ataccgataa
    59101 agaagaagtc aactatttag gtgacatgtc aggaattgat gctgacgaac tgatggcgca
    59161 atacgacaga gaatccgcat ttcgtcgtct gataggggtc cccggcaagt tagtattttt
    59221 tatcgctgtg tcatggtctg tgttccaact ttacaccggt tttttcggca catttccgtc
    59281 aacgctgcag cgtgcaccgc atttggccgc agcactgacg ttggtttatt tgctttatcc
    59341 tgccaaaagg ggaaagtctg tcgaatttac aaaaagtatt ccatggtatg attatgtttt
    59401 ggcggcagca agcataactg tcggttctta tcatattatt ttttatgatg cactcctcct
    59461 gcgagcaggt tcgtttacac agttagatgt tgttgtaagt gtcatagcta ttttgcttct
    59521 gcttgaagca gctcgacgag ttgccggtat ggtaattgtc acagtaggaa ttgtatttct
    59581 tgtatatgca ctatttggtc aacatcttcc gagaacatca ttcctcttcc atgcaccgtt
    59641 tacttttaaa cgtgtggtca gtacggaatg gttgggtagt gagggtattt taggaacccc
    59701 gatttatgta tcatcaacct ttatcttctt gttcttagtg tttgcgacct tcctgaagaa
    59761 gagcggcgta ggagattgga ttacaaatat tgctttggga gctgtcggag gtcaagtcgg
    59821 cggtccggct aaagcagctg tcgtagccag tgctttagag ggaactgtaa gcgggtcttc
    59881 tgttgctaac gttgtcggct ccggttctat aacaattcct ttgatgaaaa acacgggtta
    59941 tgaagcaaat tttgccgggg cggttgaagc agcagcttca acaggaggac aaattatgcc
    60001 tcctataatg ggtgctgcag cattcatcat gacagaatat gtaggtgtac cttatgctac
    60061 agtcgctacg gctgctgtta tccctgctat attatatttc accggtatat ttttgaatgt
    60121 tcactatgaa gctgttaaga ccggtttgag aggtattcct aaagagcaac gccctaatac
    60181 tatgaagttg tttaaagaag gctggtatat gatgacaccg gtaatacttt tgtttggaat
    60241 gcttattggc ggaagttctg ctatgaaggc agcgatgtat ggtataattg gttgtataat
    60301 tgtatggatt gttaatattc ttaaggaaga gagaaaattt gatgttgtaa ggttcgtaaa
    60361 aatgtttatt gattccctcg atgagagtgc tcgtagtgcc gtaaccgtag ctattacctg
    60421 tggtaccgca ggaatcatcg ttggtgttgt taccatgacc ggtctcggcc ttaagatggc
    60481 tgatggactg attgctttgg ccggaggcag tttgcttctc acaatggttt ttacaatgtt
    60541 cagctcgctg atacttggaa tgggtgttcc gactactgcg aactatatta ttcaagcaac
    60601 gatttctgct ccggcacttg tagctttagg tgttccgccg attgctgcac acatatttgt
    60661 attctatttt ggtatcgttg ccgatataac tcctcctgtt gctttagctg cttttgcagg
    60721 tgctggtata tcaggcggaa atccgatgaa gacaggattt attgcaacac gtctcggcat
    60781 cgcagcctat atcgtaccat atatgttggt gtataatcct attttggtac ttgtaaacac
    60841 cgaaggttat tcaattccta tatttacctt agtagtaata aaatctgccg taactgctgt
    60901 tattggtatg attggtcttg ctaccggatt cacaagatat ttcaaaacaa attgcaagat
    60961 ttgggaaagt attgttttaa tcattgcggg tttgttgctc gtcgacccgg gtacagctac
    61021 cgatattgcc ggtgccgttt tgatgtttgc gatttactta gtacagaaaa gacgtatcgc
    61081 gaagactgca atttcgtagt atttgatata tataatcgac atcagttcaa tcttgatatg
    61141 ctgtctcgta ggaggttgtg aaacgtaatc tcctgtggag atctttcaaa ataatgaggg
    61201 taaatctact gatttaccct tagattgttt atcaggcaaa ttttgacctg gtcaatgatt
    61261 tgatgcagat aattaaaggc aagacaattg agtttgcata taatatcatt tgaattttac
    61321 ctgaataaag gggagtatga ttggtatggg caatattaca atatacagga attaatttat
    61381 gggttaagag ataaaaacaa tgaatatggg gagaattata atgattagag aaatgagaag
    61441 aaaagataga gaactgaaaa atgatgaagc tgtcgaaatt ttaaataact tatttattga
    61501 ataataatca aatacagtaa taagatatca attctttatc ggtagcaatt gatagtggtc
    61561 aatttttgtc ttttgataaa tttttggaat agttgtaaaa actcatgagg attttattat
    61621 attaagaagg tttgacagga aatggcttgc tgtgataaac tgtaaaaaat attatactaa
    61681 aacaaacggg agttgataaa atgtcattaa gcgaaattat tcgtgcgata ggctttttca
    61741 tagcagctat tgctatctac cttgtgttat tagtaattac aatgaaaagg aatataataa
    61801 aagttattaa agtatttgaa aataagaacg ccttatctgc taaaacagct ataagctaca
    61861 cagagcttaa tattcaaaaa cagggtacta ttgatcgtgc cataaaaaaa cgcgacaata
    61921 ggtctcatgc cctgaagttt ctgttggatg gaggagttgt tattataaac ggtgatgaaa
    61981 gatactatct caataaaaag aaaatgcttg catttaaaca acgtttgaat tttattggac
    62041 gtatgatgat tcctgatatg gacgattaaa cttgcaattt actagcttct tttatattac
    62101 gaaaaacttc tggtataaaa attgtctata tactataata agaggttcac ccaacaccaa
    62161 tattgtaaaa ataataaaat attgtcaaac cttcgtactt ttgatggttt ttttattatg
    62221 tcaagaatga taagctattg acaaacaaaa atttgtatta taaggtgaat aacatggata
    62281 atactgataa agtatggagg aaagatgaaa aatttgctga ttgtcgagtc cccggctaaa
    62341 gctaaaacaa tacgtgatat tttaggaagc agttatattg ttaaagcttc ggttggacat
    62401 gttcgtgatt tgcctaaaag caagctgggc atagatatag ataatgaatt ccagcctgat
    62461 tatataacaa taagaggaaa gggagatata atcaaggagc ttaaaaaaga agcaaaaaaa
    62521 gctgacaatg tgttccttgc aacagaccct gacagggaag gagaagccat ttcctggcat
    62581 ctggctaaaa ttttaaattt agatcaggaa aaggaaataa gaattgtatt taatgaaatt
    62641 acaaaggatg caatcagaaa tgccgccagg gcaccacgca aaattaacat agacctggta
    62701 gatgcccagc aggcaagacg tgttcttgac agactggttg gatacaaaat cagccctctt
    62761 ctgtggaaga aaataaaaaa gggtttaagt gcaggcagag tccaatcggc ggcattgaag
    62821 cttgtaatcg acaaggagcg ggaaataatg gattttaagc cggaagagta ctggtcttta
    62881 gagcttgaac taaaaaaatc aagaaaaaaa ttcactgcca gatatgtggg aattttcgaa
    62941 aatgacaaat tgaaaaaggt tagaattaaa aatcaagagg aattaaacag aattcttgaa
    63001 agcattgata aagaaaaaat atccgttgca aaaattgata aatccaaaag cttcataaag
    63061 cctggaccgc cctttacaac ctcctcactc cagcaggaag caaacagaag gctgaatttc
    63121 acatccaaaa aaacaatgat ggttgctcag cagctctatg aaggagtgaa agtcaagggg
    63181 gaaggaaata tcggtcttgt tacctatata agaacagact cattaagatt atctgatgaa
    63241 gcaatagaca atgcaaaaaa ttacatttta tcaaattacg gagaaaaata ttataggtac
    63301 aggttatata aaaaagcaaa gaaaaatgaa agcaaggttc aagatgccca tgaagccata
    63361 agacctacct ccttgctcag gtcaccggaa aggctcaagg acagccttac ttccgaccaa
    63421 tacaagcttt acagtctgat ttggaaaaga gctcttgcct gccaaatgca ggatgcagaa
    63481 tatgatgtga caaaggtttt gtttaacaat aatgacaata tatttgaaag cagcggaaag
    63541 atacttgtgt tcgacgggtt tagaactgtt tacggctata ctgacgagaa ggatgaaaaa
    63601 atgctccctg aactggaaat aaatgaagta ttggaaatcc tggggattct tccggaacag
    63661 cattttaccc agccgcctgt acggtataca gaagcaagcc tcataaagga tctggaggaa
    63721 aagggaatag gacgcccaag cacttatgca ccaactatta caacaataac aagcagagac
    63781 tatgttgcta aggaaaaagg gtaccttttt ccaacggaca tgggtttttt ggttacagaa
    63841 atgatggaga aatattttcc caaaatcgta gatgaaaagt tcacggccaa tatggaagac
    63901 cgcctggatg acgttgcagg tggaaatgtt aactggccca gcataatttc tgaattttac
    63961 gaaggattta agaaagacct ttttgttgcg gaaaatgaaa tgaaagaaat agaagtaaaa
    64021 gaagaagtaa gtgatgtaaa atgcgagaac tgcggtgcct tcatggttgt caaaagcggc
    64081 aggtacggaa aattcctggc atgcccgaat tatcccggct gcaagaatac caagccctta
    64141 aatggcaggg aacctgctga ggaaaccgat gaggtctgtg aaaaatgcgg ttcaaaaatg
    64201 cttaaaagga agggaaagtt cggcgaattt ttagcgtgct ccaattatcc aaaatgcaag
    64261 aatacaaaac ctattaataa taccatagat gccttatgtc ccagatgcgg aaataaaatt
    64321 gcagttaagt attcaaaagc cggacggaaa tttttcgggt gctccaatta tccggaatgc
    64381 aattatattt catggtatga gcctacccgg aaaaaatgcc ctgaatgcgg ggacttgatg
    64441 gttttaaagg acaacaagga tgtatgtgta aataaaaaat gtaaaataag tgaataatat
    64501 ttatatcagg taatatatac attttatatt cacattttta ataattaaat attgccaaac
    64561 aaaatcggct gtgatatact aatcataatt acgcacagat ttggatcatg ttagagttgt
    64621 gccggaaacg gtctttttca tgatatgaca tttctggagg taaaacaaaa ggaggaaaca
    64681 aaaatggcta taatcagcat gaaaaagctg ctggaagcag gggttcattt tgggcatcaa
    64741 acaagaagat ggaacccgaa aatggcagag tacattttca cggaaagaaa cggaatctat
    64801 attatcgact tgcagaaaac aagcgataaa gtggatgaag catacagctt tatcaaggaa
    64861 gttgtatcag agggagacga aatattattc gtcggaacaa aaaaacaagc tcaggaatca
    64921 acaaagacgg aagctgcaag atgcggaatg cattttgtaa gccagagatg gcttggcgga
    64981 atgctgacaa actactcaac aatcagaaaa agaattgaaa gactcaatga attgaaaacg
    65041 atggaagagg acggcacatt caaactcttg accaaaaaag aagttttcaa attgaaaaat
    65101 gaagctgaaa gactggaaaa attcttaggt gggatcaaaa atatggataa actgcctggc
    65161 gctctgtttg ttgttgaccc gagaaaagaa aaaatagctg tccaggaagc aaaaatattg
    65221 ggaattcctg tagtagcaat cgtagacaca aactgcgacc ctgatgaagt agattatgtt
    65281 attccgggca atgatgatgc aataagagcc gtaaaattat tgacggcgac tgtagctgat
    65341 gctgtgcttg aaggcaaaca aggcgttcag atgacttatg acgatgttga agaagacgac
    65401 gaagtatctg atgaagaatt ggaaaaagtc gaagaagaag aatttgaatt ggaataaaaa
    65461 caaaaatcaa gtaaggggtc aggaattatt tccttacgcc ttacttttaa attatggagg
    65521 atatttaaat ggaaattaca gcatcaatgg ttaaggaact aagagataaa acaaatgcag
    65581 gcatgatgga ctgcaaaaaa gcattgaagg aaacaagcgg ggatatcgaa aaggcaatta
    65641 tttatttaag agaaaaggga ctgctgcagg cagcaaaaaa atcaggaaga atagcatcag
    65701 aaggccttac aacagcaata atttcagcag acggggaaaa gggagttgtt ctagaagtaa
    65761 actcagaaac tgactttgtt gcaaaaaacg aagaattcag gaattttgtt acagctgcag
    65821 cccaggcagc tcttgacaat gagcctgcag atttagaggc attaaaggct cttgtgcttg
    65881 aagacggaaa aacagttcaa gatgttttaa cagataaaat agcaaaaatc ggcgagaata
    65941 tgtcactgag aagatttggc cgggcaaaag ttcaaaaggg tctggttgta gactatattc
    66001 acgccggcgg taaaatatcc gttcttgtag ctttggaatc tgaaggggac aaagaaacac
    66061 taaaagtttt aggcaaggat ctggcaatgc aggttgcagc catgagcccg aaatatattt
    66121 caatggaaga tgtagatgat gagttcataa gacaagaaaa ggaaatactt acagcccagg
    66181 cattaaatga aggcaagccg caaaacatag ttgaaaaaat ggtagaagga agacttaaca
    66241 aggaacttaa ggaaatatgc cttttggagc aggcatttgt taaggatgct gatttaaccg
    66301 taaagaaggt aataagcgaa acagcaaaga aaatcggcgc cgatattaag gtagctgatg
    66361 ttttaagata tgaagtggga gaaggaatag agaaaaagtc cgaaaatttt gctgatgaag
    66421 tagcaaaaca aatgggttta taatcctgta caataatgga gaaccaaggg ttctccatta
    66481 aataaataca atcaggagga aatgctatgt gcaaatataa gcgaatagtg ctgaaaatca
    66541 gcggtgaagc cttgtccggc agcacaggcc acggcataaa ttcagaaata gtaaacagaa
    66601 tttcaggcgt aattaaaaga ataaaccaaa tgggagtaga agtagctatc gttgtagggg
    66661 gcggaaattt ctggcggggt gccggtgcca gggatatgga cagaacaaca tctgattata
    66721 tgggaatgct tggaacaata ataaacggac ttgccctgca ggacgcactg gaaaaaatag
    66781 atgtggaaac caggctgcag acagctattg aaatgagaca aatagcagaa ccctacataa
    66841 ggcggagggc agtaaggcat ttggaaaagg gaagagttgt gatattttcg ggaggttcag
    66901 gcaacccgta tttttcaacc gatacaacag ctgccttaag agctgctgag ataaatgcag
    66961 aaattatcct tctggcaaag aacggtgtag acggtgttta ctcagatgac ccgaaaaaaa
    67021 atcccaattc gacaaaattc gaagaactca aatatattga tgttttaaac aaaagtttaa
    67081 aaataatgga ctcaaccgca acttcattgt gcatggataa caaaattcct attttggttt
    67141 ttggtataga aaaccccgaa aatattgaaa aaattgtttc aggagaaaac ataggaacta
    67201 taattaagga gggatagtta tgtatatgga tctaatattt aaatcagagg aattaatgag
    67261 aaaatccata agttttctca aggaggagct tgctacaata agagcaggca aagccaatcc
    67321 gaaattagtt gataaaattc aagtaagcta ttacggtaca atgacacctt taaatcaaat
    67381 agccaatatt tcagtacctg agcccaggat gcttctcatc cagccctggg atgcacatgc
    67441 cttaaaggac attgaaaaag ctttgcagac atccgatttg ggaataaacc ctgcaaatga
    67501 cggaaaatta ataagacttg taatcccggt tctaacagaa gaaagaagaa gagatctgtt
    67561 aaaagtcgtc aagaaagaaa ttgaaaattc taaaattgca gtccgtaata tcagaagaga
    67621 tactaatgat gaaatcaaga aacttgaaaa acaaagcgaa atctcaaagg atgacctgaa
    67681 gaaagctgaa gaagaaatgc agaagctcac cgacagatac atagacatca tagatgaaat
    67741 atataaagat aaggaaatag aaattttaga ggtgtaaatt ggatagttta accggttatc
    67801 cgttaaaaga agcacttggt gaactggaaa gctcaaattt aattgttaac atagttaaag
    67861 ttacaggcag aaataataga ttcaacaacc tggaaaaacc atatgtcata agggatattt
    67921 attcagatgg tacggtcacc ctgtatatat cttattttta gtgtatttga aagaggttta
    67981 agatgctgga agaaaaagta ttatcaggag aaatacctga acatattgca gttataatgg
    68041 atggaaacag aagatgggca aaacaaaaaa atctgcctgc aaaaatggga cacagggaag
    68101 gtgctttaag ggtgatcgac cttgtgagaa cctgcggaga cataggagta aaattcttgt
    68161 ccgtctatgc cttttcaact gaaaactgga accgggacaa aaaggaaata gattatttga
    68221 tgaatcttct ggttgaattt gtattcaatg agcttaatac cctgcataaa aacgacgtta
    68281 aaatcacaat gatggggaat atcgaggatt tgccggataa aacaagaata gaagtggaaa
    68341 gggctgtaga acttaccaag aacaacaaga cacttcatct gaatattgca ttaagctacg
    68401 gttcaagaaa tgaaatatta agagctgtta aaaatatagt taaagaatgt ggaaacaaag
    68461 agctcggcat tgatgaaatc aatgaggaaa gttttaaaag atatttgttt acatataata
    68521 tgcctgatcc tgacttattg atcaggacca gcggtgaaat aagattgagc aactttcttt
    68581 tgtatcagat ggcctatacc gagttttatt ttacagaggt tctgtggcct gattttaata
    68641 aaaacgaatt atttaaagca attgaaagct atcaaaaaag aaaaagaaga tacggcaatt
    68701 aggcggtgtt ctatgggaca aagagtgtta accgggttta tagggggagt atttatagct
    68761 tttgtaacat atttaggcgg ctctctgtat gattttgttt ttttggctat aacatgcata
    68821 gctatttatg aattatcaaa aatatttttt ggcaatggtt ttgactatgg tgccatagtt
    68881 aattattttt ttgcaatggc tttgtttatc gagaaaataa cggaaaacag gcactttttc
    68941 gatttttttc tttttttata catatcagtt aactttttga tatttgtatt taacaggaag
    69001 gtaacaatta aaaaggtttc agagatatta tttttgggta catatgtagt gttcttcatg
    69061 tatcacatga tgcttatgaa tagttcaccc tttgtatggc ttgtttacat tattgcattc
    69121 ggttcagata catttgcata ctttaccggc aagtttttcg gtaaaaacaa actgtatcca
    69181 gaagtaagcc cgaataagac aatagaaggc gcaataggcg gtattgccgg aagcacaatt
    69241 ttatcactga tattttttaa ttatttaagc ataaataaat atttttacat tataatattt
    69301 agtgtaagtg catcagtatt ttcaatggcg ggtgatctta cagcatctaa aataaaaaga
    69361 gaatacgaaa taaaggattt tgggaatctt ctgcccggtc acggaggtat aatggacaga
    69421 tttgacagtg tcttatttgt tgctccggca gtgtattatt ttattttgca ttttctataa
    69481 acggagggga aatattggta acattaatag catcgattct tgtcttttct gctattgtcc
    69541 tggtccatga atatgggcat tataaggcag ctaaaagtgt aggtataaag gtatatgaat
    69601 ttgccatagg aatgggacct accttaatta agcgtgagaa aaacggaaca ttattttcaa
    69661 tcagagccgt tcctataggc ggttttgttc tgatggaagg cgaagaggag gattcaagaa
    69721 caccaacaag ctttggaaca aaatctatag gccagcgggc taaggtaatt gcagcaggtc
    69781 ctgttatgaa ctttgtatta gctttggtcc tgtttctcat aattgccttt ggttatggag
    69841 tttatggaaa tcatgtggat ttcatcgacg aaaattccaa tgaatatatg gaaggattaa
    69901 gaacaggaga taaaattatt tccattaatg gtgacagaac atatatatgg gaagatattt
    69961 tttatgaatt aatatcaccg gaggaaaact cctacaccat tgaatatgaa agagacggca
    70021 aaataagcaa ggcaaatata gacaagagct acagaaaaat cataggaatt acctcaactc
    70081 ttgtaaacgg ccagacaact acaaccgtta agccttcaaa tattacatcc cctgcataca
    70141 aagcaggcat tatggagggg gatgtagtga taaaaattaa tgatatatat gtaaatacat
    70201 gggatgagct gataggtcag gttgaaaatt caaataaaac agtcaaggtt accgtggaca
    70261 gaaagggtga gattttagat tacaatattg acctccagga gcaaatatca gttagattta
    70321 atactcagct tgaaaaatca tttattacag cgattacttc ttcaatgtat aaaaccgtgt
    70381 attatattga acttatgttt aaaacaataa gacagctggt aacaggaaga cttggcagcg
    70441 atgccctggg cggtcccgta atggtaatat caatggtagg ggaagcagct caaagcggaa
    70501 ttctttcact cttgagcctt gcagccttta taagtattaa cctgggcttt atgaatctat
    70561 tgcctgttcc tgcactggac ggaagcaagc tgatattttt agctgtagaa ggaatcaggg
    70621 gcaaggcact gcctatggaa aaagaaggct atattcattt tataggtttt gtatttttaa
    70681 taggttttat gctgtttata acctataagg atatattgag aatatttagg atttaaaaat
    70741 tttctgaaag gaaaattatg gataaaataa tcagaagaaa aagtaccggg gttatggccg
    70801 gcaatgttca aataggtgat ggtgcagata ttactgtcca gtccatgacc aatactttca
    70861 ccaaggatgt aaaagcaaca gtagaacaga ttctccggct ggaagaagca ggatgtgaaa
    70921 tcatcagggt ggcagtggcc gactatgagg atgctgaagc aataaaagaa ataaaaaagc
    70981 aaattcacat accccttgtt gcagatattc atttcgacta tagacttgct ataaaaagca
    71041 ttgaaaacgg aatcgacaag cttagaatca atcccggcaa tataggcggc agagacaggg
    71101 tgaaaatggt cgtggaacat gcaaaaccca gaaatattcc aataagaata ggtgtcaatg
    71161 ccggcagcat acacagggat attttaaata aacataatgg atttcccact tctcaggcaa
    71221 tggtggaatc tgcgctggaa catgttcgta ttttagaaga tcttgactac aataatattg
    71281 taatttccat gaagggcact gatattaaaa cgaccgtcaa tgcatataaa attatggcag
    71341 aaaaggtttc ttacccctta catataggaa tcacccatgc gggaactttg tttgaaggaa
    71401 gcataaggtc agcaatagga gttggcagtc ttttaaccga aggaatcggt gatactgtaa
    71461 ggatatcatt aaccgatgac ccgggagaag aagtaaaagt tgcaaaagaa atattaaaaa
    71521 gtctaaattt aagaaatttc ggaataaatt atataacatg tcccacctgc ggaagaacta
    71581 aaatttccct tattgagctt acaaaaaaaa tacaggaagg attaaagggt gtcaataagc
    71641 caatatctgt tgcaataatg ggctgtgccg tcaacggccc cggagaggcc agacaggcgg
    71701 atataggaat tgcaggcgga acaggggaag ccttattgtt taaaaaggga gaaataatag
    71761 gcaaggtaaa ggaagaagat attgtaaaaa ccctgctgga agaaatagat aaaatcccct
    71821 gaggttgaca tacaaataaa atatgtagta taatttattt atgggataca gttatatcaa
    71881 aattaccgcc actgtttaaa ccaggcgttt ataaaaaata gcatggctaa ggaggaaaaa
    71941 aatggcagta ggtgctttaa atgtagaaaa gagattaaaa gaaagcagca aaacaaacaa
    72001 acaactcaga agagaaggtt ttttgcctgg cagcatatct agcaaaggaa aggattcagt
    72061 gtcagttaca ataaaggctg atgaactgag aagaggctta tccacttacg gaaggaattc
    72121 tttgtttaga ataatattgg aaggcaggga aatcaccggt atggttaaag atattcagct
    72181 ttcgcctgtg aaaggtacta tgctgcatgt tgattttcaa gaagtatcat taacagagga
    72241 aatcaaggta gttttacaaa ttgtgctaag aggaacagat gccctggaat ttaaaggact
    72301 tatggcatta agacaatcag attcggttac agttaaagga ttgccccaga atattcctga
    72361 tgacttggta attgatgttt caaatattga cagagttgaa aatatacact tgaaagatgt
    72421 ggcttttcct gaaggaataa ttcctgaagg cgaccctgaa catcttttaa tttctattgc
    72481 tgaagctaaa agaatagctg tggaaagaga ggatgaagaa gcatccgaag atgtggaagc
    72541 tgcagcggca gatcaggaaa acaaagagga ataagattga tactgcacgt tagtacacca
    72601 taaaagtctc aataaatgag ctttttcagg tgtactcttc ttgcactgtt gataatgtat
    72661 ttgattaaaa tatattctaa agaaatacag gagaaaagat tatgaagtgg aaagggagaa
    72721 gaggaagttc aaacgtacag gacagaagag gcggcggcac ttcaagaggt gttgtgggcg
    72781 gcggaatagg gacaatcata ttgttcgtag ttgttttgtt aatgggagga gatccttctc
    72841 agttattagg caattcagca tttataaacc ctgcaccgtc tcaatatgtg gaatcggaag
    72901 aagaaaacga attagcggaa tttgcatctg tcgtattggc agaaacggaa actgtatggg
    72961 atgacctatt cgaaaaggaa ggtcttgaat atcaataccc aaccctggta ttatatacag
    73021 attatgttca atcagcctgc ggcaatgcaa cttcgcaaac aggaccattt tattgtcccg
    73081 gtgacaacag cgtatatatt gatttaagtt tttacgaaga attaaagaaa aattataaag
    73141 ctcctggtga ttttgccttt gcctatgtaa ttgcccatga agtaggacac catgttcaaa
    73201 agcaattagg aattactgac caggttatgg gcttaagaaa caggctcagc gagaaggatt
    73261 tcaatcaata catggtgaga ctggaactgc aggcagatta ttttgccggt gtttgggctc
    73321 attatgttga aagggcaaat cttcttgaaa aaggggatat taatgaagcc ctcaacgcag
    73381 ccagtgcagt aggtgatgac cgcattcaaa gtgaagcctg gggatatgtt gttcctgata
    73441 aattcactca cggcacatca gaacaaagag cacgctggtt ctacaaaggt tttgaatcag
    73501 gaaccatgca agacggagat acttttaacg caaaggattt gtaaaataac aattgcatca
    73561 gtaaaacagg gacattcctt ataaggaatg tcccttattg tttataattc ttctatatga
    73621 ataagccctt caaaggaaga aagcttctgt ataatatcta tatggcttat tcttttgttt
    73681 gcttccagtg taaaaatagc tataactgat acttcatgct gtacggcatc ctttgtaatt
    73741 tcaatgtcct ggattctgaa acctgtttgt ttgcagaaaa ctatgaattt atccaattgt
    73801 tccacagacc cgaaagaagt ataaacattg ataattctgt tggttgatgt cattatatag
    73861 tcaatttttt tgaaaaaggt tattactaca aatacaacca aggcaaccat aacagcacct
    73921 gagtaaaatc caactcctat tgcaaggccc atgcaggctg atgcccataa acccgcagcg
    73981 gaagtaagac cttttatctg gcttctgctt gttaccatga ttgttcctgc tcctaaaaaa
    74041 ccaatgccgc tgatgacctg ggctccaagt ctggcaggat cgcttcctgt aaaattattg
    74101 taaatgtatt gatttgtcat cattaccaaa gcagagctca cgcatactat catataagtt
    74161 ctaaagccgg caggcctgtt ttttctgctt ctttcaaggc cgattattcc cccgcatatc
    74221 atggccagta taatacgcat ggttacagag taaatattaa gctcatacaa catattccat
    74281 acctccttgc atattcattg tatactaagc acattgccaa agtttttgat tcattagctg
    74341 tattcattat atcatatatg ataatagttt ataaaatatt aacagattaa ataaaaaacg
    74401 gatacaaatc cgtttttatg ttcacgcagc ctggagcgcc cgaaatctca ttcgccatgc
    74461 agtctcattc accaggtatt tactttaaaa ctcttcagga agccatgcct gactgtctgc
    74521 aacttccagt tcgaatattc ggccggaatt ttcaggatcc tttgtctggt gatatttaaa
    74581 aatcattttt ccttcattca tagagcctaa aatctcaatt ttgcctgttt tgtggctcat
    74641 ggaatatctg aaactctttg ccattccgct caagttattt tttgcttcat ctatcagcct
    74701 gcatcctctc ttaagcggta cctggaattg attcttaact cccgaaaccg gcctgcactg
    74761 gaaaacataa tagggaacaa cacctgttcc aaccaggtcc ttcattagtt tttcaagggt
    74821 ttttgaattg tcatttacgc cttttagcaa aacagtctgg tttcttatga taattcctct
    74881 gtttattaat tctttgactg ctctttttga ttcctctgtt atttcatttg gatgattgaa
    74941 atgggttact acgtatattt gttttatttt gctgtaacgt tccaatatat ccagaagctc
    75001 agggtcgctg tatattctca tcggaaaaac aacaggaatt cttgtaccga agcgtatata
    75061 taccaaatgt tctatttttg acaattcctt taaatagttc tcaatctttt ggttgctgtt
    75121 taaaaatgca tctccgcctg aaatcaaaac atttgtcata tctatatggt ccttgatata
    75181 agatatgata tcttcaaaat gtttatttac ttcatcgtca ttctggccca caagcctttt
    75241 tctgaagcag tgcctgcaat acatggcaca attgcttgta gaaagaagca tccccgtttc
    75301 cttgtactta tgctgcagcc ctacaactac agtgttgtcg gcttcaccgc tggtatcaaa
    75361 gctcccgctc atgtctgtct cgcttatgga tggtatgcaa agctttctga cagggtcatc
    75421 cggatcatct ttatcaataa gggataaata gtatcttggt acagacatgg gatatctttc
    75481 aataatctgc gtgagtttgg ccgtttcttc atctgttaat tttgcattta aagcttttat
    75541 cacttcttca gcagtagtta aattatttaa taattctttt ctccaatcca taaatacatc
    75601 tccttttatt acttttgcat aaccgaaaat cagcggtcaa tagaaaaaac tataatgtgc
    75661 tcttaatata ttataatctt attgaagtaa attattttat agagaagtta aattcaccgg
    75721 taaaaatcaa aatcggaatg catatatgat atttaatgtt accactaaaa acgaaattag
    75781 taaagcgatt tcatgctatt tttcgaaata tttactaaac gtaattatac aaaacgtaat
    75841 catttataat tgtttgttaa aatatgttat ttattgtatt taatttttta ttttgttata
    75901 ctgattgtta gaaagataag ggagaatgct atgtttttaa aagatgcatt taaagataag
    75961 ggacttgtta atatagaagt tgaaaagatt gaagtgtcag aaaataatga aaccgtaaaa
    76021 gttcacattt taagcagaga aatcatcggc ttaaatatta tagaaaacat tgagagccag
    76081 ctaaaacagc actatgacaa tgctgtggat tttaaactgc atatagaata taaccttgac
    76141 aaaagtgcaa agaaggaaat tgaaataatt aaagacaata tatttcacat catccaaaaa
    76201 agttccagta tcctggccaa agcaaaagac aacataaata tcgaatttaa agatgactgt
    76261 ttttttataa ctgccaataa taagcttctt ataaacgagc ttgatgatag caagacatta
    76321 ggaaaaatca aaaaattaat ggatgaatac aaaatagact gccaggcaaa aacaatatat
    76381 caagaggcgg aagatgaagc agcaattaca gcccaaagaa tattcgatga ggaattaaaa
    76441 aagaatcttg aattgatcaa taataatagc tcttcagaaa aaaagcctgt caataattca
    76501 caaaaggata atttcaggaa aagttcaaga aaaaaatttg actttgacag cctgcctgtt
    76561 tctcttattt cagacattac aattgattca ggccttgtta atattaaagg gaaaattgta
    76621 aatgttgaaa aaagagagct gaaaaagggc tccttccttt tgttggtcag cttaacagat
    76681 tctacggata caataatgtg caagtacttt tgtcccaaga atttcgatga tacaggaata
    76741 gaaaagggta aatacctcca ggctggggga aatgtggaat ttgatgaata tgaaaaagaa
    76801 gagatagtca aggttcagga ccttaggctt ataagcgaag aaatcaaacg gatggacaat
    76861 tcaaaagaaa aaagggtaga gcttcatctc catacaggca tgagctccat ggacggcatt
    76921 aacaacttca aggaatatgc ggacaaggca aagctctggg gccacagggc aatggccata
    76981 actgaccacg gtgttgtaca gggctttcct gatgcaatga aatgtgcaga cgataatttt
    77041 aaggttattt tcggcatgga aggatacctg atagacgaca cggttaatat tgtacataat
    77101 tgtgaggata taaggctgga ttctgatttt gtcgtatttg acctggagac taccgggtta
    77161 aatccaaaag atgaccacat aatcgaaatc ggagcagtta aaatttcaga caggaagttt
    77221 gtggacagct tttcaacctt ggtgcatact gataaaatgc tttctgaaaa aataattgaa
    77281 ctgacttcaa tcacagatga catgcttgaa ggacagccgg atataaatga agcactgggg
    77341 gaatttttga aatttgcaaa aggcagtgtc ctggtagccc acaatgctaa atttgacaca
    77401 ggtttcttaa gagagaaatc aaaggcggcc ggaattgaaa attacgaccc gccaaccctg
    77461 gatactttgg aactgtcaaa agcattgatt aaagacataa aaaatcataa gctgtctacc
    77521 ttgaccaaaa aattgggcat aaaccttata aaccaccaca gggcagtaga tgatgcaaat
    77581 gccgccggag agctttttat aattctgttg aacaagctca aggatagaga aatatttgaa
    77641 atttctaaac tgaatgaact ccttaaggaa gatatcgata ttgtcaggca gccatcctac
    77701 cacatcaata tactggtaaa gaataaaaag gggcttaaga acttatacag attagtttcc
    77761 gattcacata taaataactt ttataaaaag ccaagactct taaaatcaaa tttaaacaga
    77821 tttagggaag gcttgatgct tggcactgcc tgcgagaccg gagagcttta cagggccctt
    77881 ttagagggtt acagcgatga aaaaatcgaa gagatagttg attattatga ttttttggaa
    77941 attcagcccc ttcaaaacaa tatgtttctg gtaaacagcg gaagattaaa aagtgaggaa
    78001 gaacttaaag aaataaacag aaaaattatt agcttcggag ataaaagagg gaaattgaca
    78061 gctgccacag gagacgttca cttcctcgaa acagaagacg aaatattcag aaaaatttta
    78121 atgtcaggcc agggatatga agacgctgaa gagcaggcac ccctgtattt taaaacaacc
    78181 ggtgaaatgc ttgatgattt ctcatattta ggtgaagaaa aggcttatga ggttgttgtt
    78241 aaaaacacga atttaattgc tgatatgatc aaggatgtac ttcctattcc aaacggtaca
    78301 tttcctcctt caatggaagg atctgaccgg gatctcagaa aaatatgctt tgataacgcc
    78361 cggtcaatat acggggaaag cctgcctgaa atagttgaaa gcagattaaa cagggaatta
    78421 aattcaataa tcggcaacgg ttattccgta atgtatatga ttgctcagaa attagttaaa
    78481 aaatccaatg aagacggcta cctggtcggc tcaaggggat ctgtaggctc atcctttgca
    78541 gccacaatgg cgggaataac cgaagtaaac cccctggtgc cccattatgc atgccccggg
    78601 tgcaaatata ccgagtttat tgaagacggc tcctacggct ccggatttga cctgcctgaa
    78661 aaatcctgcc cggtttgcgg cacatccttg gttaaggacg gccatgacat accttttgaa
    78721 gtattcttag gctttgaagg tgacaaggag cctgatatag acttgaattt tgccggtgaa
    78781 gagcaggctg tatgcatgaa atacacagaa gagcttttcg gggaaggcaa agttttcagg
    78841 gcaggcacca tcagcactat tgccgacaga accgcctttg gatttgttaa aaattatttt
    78901 gaagaaagag gcataaataa gcgcaaggcg gaaatagaca gacttataca aggctgctta
    78961 ggggttaagc ggacctccgg acagcacccg ggaggagtaa tgatagttcc tcaggataag
    79021 gatatatatg attttacacc tgtacagtat cctgccaata atgaaaagtc cggaacagta
    79081 acaacacatt ttgattatca gtcaatttcc ggaagaattt taaagcttga tctgctcggc
    79141 catgatacac cgagcatcat ccgcatgctt gaggacttga caggtgttgg caaagagact
    79201 attcctgttg atgaccccga taccatgaca atatttaaca gccctgaaat attcggcata
    79261 acactggatg aaataaactg cagcaccggc accttgggca tacctgaatt tggaaccggt
    79321 tttgtaagac aaatgattat tgatacaaag ccccggtcat tttcagactt gataagaata
    79381 agcggacttt cccacggaac agatgtctgg gtcaacaatg cacaagacat aataaaacaa
    79441 ggttatgcag aaatcggcgg agttatatcc acccgtgacg atattatgac ctatttgata
    79501 caaatgggag tagaaaaaaa gactgccttt gacataatgg aaagagtccg caagggcaag
    79561 gggctaaggg aagaggacga aaaagcgatg aaggcgcaga atattccccg gtggtatata
    79621 gactcctgca atacaattaa atatatgttt cccaaggcac atgcagctgc ctatgtaacc
    79681 atgtcggtta aaatagctta ctttaaggtt cattatcctg aagcctttta cgcggcatat
    79741 ttcactacca aggttgatga ttttgatgcg gatattgtaa caagaggcca gaattatata
    79801 ttaagtacaa taaaatacct tgaagaaaag ggaaatgaca agaccgcaaa agaaaaaaac
    79861 cttattactg ttcttgaagt agcctatgaa atgtacaagc ggggctttaa atttacaaag
    79921 gttgatttat ataagtctga tgacaggaaa tttttgcttg ctaaggatgg aattcttccc
    79981 cctctcatgg gactgccggg acttggggcc aatgcagcgg caaatataaa ggcggaaaga
    80041 gaaatatccg aattcatttc aaaagaagat ataataaaaa gagcaaaggt atcaaaaacc
    80101 gtaattgaag tgatggaaaa ccacggttgc ctgaaaaata tggatgaaac aaaccaaata
    80161 tctatattta acgtgtagaa accaataagc agatatatga aaaaataatg ttgatttata
    80221 attacatttg tgatatacta ttaaaaagat agattttgac ggaaagagtg ggtgacccca
    80281 ctctttctac atgtatgacc gaaccccatt tcccgaagcc tttttcccgg aaaatggatg
    80341 gcaggtaaac tgtacagaat gccaaatttg tgcggcgaaa ggagcaaata aatttgatga
    80401 atttacttgc gaaccacatt gattagcggt atgaggaaac aagcactaaa aataggtata
    80461 tgggcataaa aggagtgaga atatacacat gaccaagatg aatatcacat cttctgtcag
    80521 gaaaatgacg gaagaagtac tgaaaactac tgaaatggaa ctggttgatg ttgaatatgt
    80581 aaaggagggt ccattcaggt atttgaaaat ttacatagat aagcccggtg gaataactgt
    80641 ggacgatact gcggatataa gcaggatttt gaataagaag ctggatgagg cagacctgat
    80701 agacgaacaa tactttctgg aagtatcctc ccctggagta gaaagacctt ttaaaaaaga
    80761 aaaagattat ttaaataaca ttggaaattt tgttgaagca agattctata agcctgttga
    80821 gggcaaaaag tcagtgaagg gacttttaaa ggaaaaaaat gaaaatgata ttgtaattga
    80881 gacaggagag ggaattctca ggattgagct cagcgacctg tcgaaaatta atagagtgtt
    80941 agaggatttt taatggaggg gttaaatgaa caaggagatt ttaaatgcat tagttgaact
    81001 tgaaagagaa aagggcatca atcaggacgt tgtcctggaa gctatcaggt ctgcattgga
    81061 aacaggatat aagaaaaatt acggcaaagc tacaaacttc agtattgaaa tagacaaggt
    81121 aaacggcgag tacagacttt accaggacaa aacagttgtg gcaggtgttg aaaacagcaa
    81181 tttggaaatt tctttggaag aagcaaaaac aattgacaaa agatatgaat tgggcgatat
    81241 cgtcaagata gaaattaaac ccaagaaaga tttcggccgt attgcagcac aaacggcaag
    81301 gcaggtaatc cttcagaaaa ttcgggaagc tgaaagagag tctattttca gtgaatttta
    81361 tggaagagaa tcagatatac tcacaggtgt tgtacaaaga gaaaataagg gtaatatatt
    81421 tatagacctg ggaaaaatcg aaggcatact ggctgtcaat gagcaaattc caggcgagac
    81481 atacaatccg ggagacagaa tcaagactgt tatcctggag gtaaaaaata cggggaaggg
    81541 cgccagcata atgctgtcaa gaagccatcc caacctggta aagaggttat ttgagcttga
    81601 agttccggaa atacacgacg gcaccattga aatatattcg attgcccgag aagcgggctc
    81661 aagaacaaaa atagctgttt tttcccatga ccccaatgtt gaacctatcg gctcctgtgt
    81721 aggaaacaaa ggaacaaggg ttaaaaatat tatcgatgaa ataaatcagg aaaagataga
    81781 tattataatt tatgaaaaag atcctgaaaa atttatagca aacagcctgg gacctgcgaa
    81841 aataataaag gtaacagcaa atgaaaagga aaaatcaaca gtggctattg ttcctgacaa
    81901 tcagctctcc ctggctattg gcaaggaagg tcaaaatgta aggcttgctg ccaagctgac
    81961 cggatggaaa atcgatatat tgagcgaatc tcaatatagc cagcgcttac aggaggaagt
    82021 atgaaggcca gaaaaatacc aatgagaaaa tgcattggct gcatggaaag ctttcccaaa
    82081 aaagagctgt gcagaatcgt aaaaaacaag gaaaatgaaa tatttttcga ttcgacagga
    82141 aagcttaacg gcagggggac atatatatgt aaaaatatag agtgcttcaa taaagcaatt
    82201 aaaacaaaac gcttgtcaaa gtcactggaa acagaaattc cggatgaagt atatgaagct
    82261 ataaaagcgg tgattgagaa aaatgcataa aatatactca atactcggaa ttgcaagaaa
    82321 gggcggaaat gtttccatag gcttcgatgc agcaaaggct gatattgaga agggcaaaag
    82381 ctttttggta gtaattgcag aagatgcatc agaaaagaca aagaaaaaca ttatatttac
    82441 ctgcaataaa catgattgta aatatatgga gttcggcaaa aaggaagaat taggtaaaaa
    82501 ccttggaaaa gaagttgtga gtgttatgtc tataagggac aaaaaccttg catcctatgt
    82561 aacaaataac atataaaccg gaggtggttg catgagaata tatgaactag caaaagaatt
    82621 ggatatgaac agcaaagatc tatgtaagtt agtcgaaaag gagttaggaa taacagtgaa
    82681 aaaccacatg agctccctga ctgatttaga agttctaaga tttaaaaaag ctttaagcct
    82741 tatgaacagc actaaaaaaa ctgtaaaagg ccctaaaaat gtggttattg atgatgaacc
    82801 cctggttgat gaaaaaatag atttaaatca ggcaaaactt aaaaaagtag acaaaaaact
    82861 cctaacaccg gttaaaaagc ctctgccatc gaagtcaaag aaggtaaaaa cgcctgcaga
    82921 gactggaaaa accgattaca ggaaggatgc tgcaaaggat aaacaaaagc agcagaaaaa
    82981 accccagcag agccgtcccg agccgaagca gcacaaagtg agaacaatca gcattgagga
    83041 ctccatcgtt gtaagagact ttgcagataa gctgggcgtt ccggtaaatg ttttaatatc
    83101 aaagcttata ctgcttggag taatggcagg catgaaccag gaaatagact atgataccgc
    83161 agcacttatt ggagaagaac taggagctaa aataagcaaa atagaagttg ttgacgaact
    83221 gcaggaccta gaggagttat tgtccaagga tgatgaagat gatgaaaaag aaatggttcc
    83281 aagaccgccg atagtaactg taatgggtca tgttgaccat ggaaagacat cacttttgga
    83341 tgcaataaga gatacttccg tcactaagca ggaagcggga ggaatcaccc agcatatagg
    83401 agcatcacag gtagaaatca acggcaagaa aataacattt ttagacactc ccggccatga
    83461 agcttttact gccatgaggg caagaggagc aaaggttact gatatagcaa taatcgtagt
    83521 agctgcagac gacggggtaa tgcctcagac agttgaagct ataaaccatt caaaggcagc
    83581 aggagttcct atcattgtag ctgtgaataa aatcgacaag cctactgcaa acccggataa
    83641 ggtaaaacag gaactttccg agcacggagt aatatccgag gagtggggag gagacactat
    83701 atttgtatcg gtttcggcaa agaaacgcat gggtattgaa gaacttttag agatgatact
    83761 tttggttgca gaggttcagg aattgaaagc caatcccaac agaagcgcta aaggcactat
    83821 tatagaagca aagcttgata aaggaagagg acctgtcgca acagttttga ttgataaggg
    83881 cacattgaaa aagggcgatt atgtattggt tggaagcacc cacggcaaga tcagagccat
    83941 gtttaactca aaagggaaga gaattaacca ggcaggtcct tcggttcctg ttgaaatagt
    84001 cggactttca gaaacgcctc aagccggaga catgctgacg gtaatggaaa acgagaagga
    84061 tgcgaaaaat atcgcagaaa agcgaaagga taagaaacac cttgaaagta taaatgtttc
    84121 agccaaggta tcacttgacg acttgtatga aagaattcaa aagggtgaag taaaagattt
    84181 aaatatcatt attaaggcag atgtgagcgg ctcaatagaa gcagtcaagc aatcacttga
    84241 aaaactgagc atggcagaag tcaaggttaa gcctatacac ggcggtgtag gaggaataaa
    84301 cgaaaatgac atccttcttg catcagcttc aaatgcaata gttattggat ttaacgtgag
    84361 gccttccgta gctgccttgg acctggcaaa acaggaaaat gtggatgtgc gtacctacaa
    84421 tataatctac aatgcaattg aagatattga agcagctgta aaaggaatgc ttaaacctgt
    84481 atacagagaa gtagtcatgg gaagagccga tgttagacag atcttcaagg tgcccaatgt
    84541 tggtcttgtt gcaggtgttt atgtgacaag cggcaaaata acaagaaaat caaaaattag
    84601 actgcttcgc aacgatatag taattcatga cggacaaatt gcatcattga aaagattcaa
    84661 ggatgacgta tctgagcttg ccagcggcta tgaaggcgga ataggcatag aaaaatacaa
    84721 tgatatcaag gaaggcgatt caatggaagc ctatgtcatg gaagaaatca aaagataaaa
    84781 gataaatgag ggtaaaaaat ggctaaaaga agaaacaata gattatcggg ggaagttaaa
    84841 agagcaatat cggaaataat cagaaatgat gtaaaagacc ctagaatttc agaacttttg
    84901 agcataactg aagtacatgt aacggaagat ttgaaatatg ccaaaatata tgtttctgat
    84961 tacaatgata tagaaacatc tatgattgcc ttggaaagcg ccaaaggctt cataagaaaa
    85021 gaaataggca aaaaagtcaa aatgagaatt ataccggagc ttatattcat aagagatgat
    85081 tccattgaaa agggaatgca catgagcagt ctcatcggaa aaattattga agaagataat
    85141 gtcagaaagg ttaatgaaga taatgcggaa gaatgatgta aaagaagtat ttgatttgat
    85201 aaataattct gacagaatct gcatagcagg acacaaggct cctgacggag actgtatagg
    85261 gtcggtaatg gccctgtaca gcttccttaa accccttggc aaacagctaa cagtgatgat
    85321 ggacggaatt attcctttta actacaagtc atttgtggat gaaagcataa tattgaaaaa
    85381 ctatggcaat gaagaatttg accttttgtt cgttttagat acaagcgaca gaaagaggct
    85441 ggggaaattt gagggagtcc tttcaaactc aaagaaaaca gttgtcattg accatcatat
    85501 aaccaatgaa ggcttcgggg atataaatat aattgagccg catatgagct ccacgggaga
    85561 gctgctgtat ggcttgttaa aacttgctga tatggagata tcaagcgaaa cggctgtttt
    85621 aatctacata gctgttataa cagatacagg caagttttct tatcaaaaca caacagcaaa
    85681 aactcacata atagcggcag agctcattga ccttggtgtc aatgtagcgg aaatagacaa
    85741 cattgtatat aattcaaagc ctgcaaatat tgtaagggca tttattgact gcataagcac
    85801 catggagtta tattacggaa acaagcttgg tattacagca atcacaaaag aaatacttga
    85861 taagaataag gccgaaatgg ctgatgttga cggtgttgtc gaatacataa gagaaataaa
    85921 ggaagttgaa gtatcctgcc tgttaaaaga aaatgctgag ggcacaaagg taagcttaag
    85981 atcaaagaac gatattgatg ttgcagccgt ttccttgaaa tacaaaggcg gcggccacgc
    86041 taaggctgca ggctttgata ttggagaaaa catggaaaat acaaaaatga ttattttaaa
    86101 ggaatttaaa gagtattttg ataaatgaca ggaatattaa atatattaaa accaacaggc
    86161 atgacatccc acgatgtggt aagtgccgta agaaaaatac ttaacataaa aaaagccggc
    86221 catgcaggaa ctcttgaccc caatgcagcc ggagtattgg ttgtttgtac aggcaaggca
    86281 acaaaattca gcgaatattt aatgagcggc caaaaagaat atgtatgcga gctgatactc
    86341 gggaaaagta cagacaccca ggacagctac ggaatagtta taaatgaatc agacaaggat
    86401 gtagtccttg atgagttaaa tgaagcttta aaaaaattca caggtgagca aattcagatt
    86461 cccccggcat attctgccgt aaaactaaat ggcagaaagc tctacgaata cgcaaggcag
    86521 aatatttcag ttgaaaaacc gggaaggaca atccgtattg aagatataaa gctccttaaa
    86581 ttcaaggata ggtctgccct tttaaaggta agatgcagca agggcacata catcagaacc
    86641 ctgtgcagcg atattggaaa aagccttggc acatacggac atatgggaat tttgataagg
    86701 actgaatcaa agggtttgca tattaaaaat tctgtaaccc tggaccggct taaattctta
    86761 agtgacagca agaggctcaa tgaatgcctg attcctgttg aagacatata caaattggat
    86821 aagctgaata ttgattccaa atattacgac agattggtgt caggcaatga agtggacctt
    86881 ggaataagca taaaaaacga tgaattttat gtatattgca gggattctct cataggtaca
    86941 ggaaaaaaaa ctgataaaac taaagttaaa attgacaaaa tgctgatata gcaggtgaca
    87001 ttatgaatga taaaaacggt tcatgcattg caataggaaa cttcgacggc ttacatacag
    87061 gacatgatgt actaatcaga agaatgattg aactgagcaa agaaacagac agggaaagca
    87121 taataattac attcaaatac attagaaacg atttgaaaaa atcttccatg aatataaaat
    87181 acatcaacag tccggaaatg aagctggcaa tattgaaatc ttacggggta gatagagttg
    87241 ttgaaattga attgaatgaa gaggtatcca aatattcgcc ggagaaattt ataaagcttt
    87301 tgaaggatga ttataatgca aaaaacattg tagtcggcta taattttact tttggacaca
    87361 aggcaatggg caatatcaat actttaatag agttccagga taagtacggc taccgtgtgg
    87421 aggaaatacc cccggtgaga tacaacggca ttgctgtaag ctctacattg attaggacac
    87481 tgctcaatga aggtaaaatt tatgaggcca atacactttt attaaataaa tatactatat
    87541 attatgatga aataaatatt gactataata aaaacatggg ttttgtagat aataaaagca
    87601 gtattttagt tcctcccgac gggaaatata ttgtcagcat gggaaggaag gaatatatcc
    87661 tgagcattgt ttcaagcaag gccggttcca gtcttatatt tgacaaaaag gttgaagaag
    87721 atagagacat agtttttata accaaagctt cataagaaaa aaaaattatt gtttacaaaa
    87781 ttatgtgaat gtggtagact ttataagttc catagactaa gtttaacgac tcctcaacgt
    87841 tttgcttgga ttatggtgaa ataattataa tatggaggtt taagaaaatg aataaggaac
    87901 aaaaaacaca gattatcgaa cagtacagaa ttaacgagaa ggatacgggc tctcctgaag
    87961 ttcagatagc tttgttgact ttcagaatca acaatcttac cgaacattta aaggacaata
    88021 aaaaggacca tcattcaaga agaggtcttt tgcagatggt tggccaaaga agaggttttc
    88081 tcaactactt gaaagaaacc gatatcgaaa gatacagaag catcatagca aaattaggac
    88141 taagaagata aacggtaaag cgggggaacc cgctttatat tttacattaa tggaggtaaa
    88201 aggtggaaaa aatatttaaa tacatacttg ccggaagaga attaaaagta actgttggca
    88261 aaattgccga acttacaaac ggatcctgta tagtacaata cggtgatact gttattatgg
    88321 caaatgtggc ggcttcaaaa gaaccgaggg aaggtatcga ctttttcccg ctgagcgtag
    88381 attttgagga gaaactatac tctgtcggaa agatacccgg cggtttcatt aaaagagaag
    88441 gaagaccggg agaaaaggca atcctgacgt cccgtttaat agacagacct ttgaggccgc
    88501 tttttccaaa gggctacagg aacgatgttt ctgtaactgt aacggttttg tcggtagacc
    88561 aggactgctc gccggaaata gcaggaatga tagggtcatc aattgccttg tccatatcag
    88621 acataccctt tgaaggacct acggggtcag ttaatatagg gcttattgac gggaatttca
    88681 taataaaccc caacagcagc caaagggaag caacatctct tgatttaacc gtatccggaa
    88741 caaaggaagc aatcatgatg gttgaagcag gagcagatga agtgaaagaa gaagtaatgc
    88801 ttgatgcaat acttttcgca cacgaagaaa tcaagaaaat ctgtgacttc ataagtgaga
    88861 ttacaaaaga ggtcggcaaa gaaaagtcat gttatacaaa ctttgtatgt gatgagacta
    88921 ttgaaaaaga aatcagagaa tatgcagctc ctataataaa ccaggcgatt attgaggaag
    88981 acaaactgaa aagacaggaa aatattgaca gctttaatgc aaaagctaaa gagattttcc
    89041 tggaaaagta tcctgaaaac ggtcaggata tcgatgatgc cctttattcc ataattaagg
    89101 aaaaggtaag agaaaatatt cttgtaagag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    89161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    89221 nnnnnnnnnn ncccggcaga agagaaatag gtcacggagc attggcagaa agagcattgg
    89281 tgcctgtcct gcccaatgaa gaagattttc cttacaccat aagacttgtt tccgaggttt
    89341 taagctcaaa cggttcaaca tcacaggcca gtgtgtgcgg ctcaactttg tcgctgatgg
    89401 atgcaggagt tccaattaaa tcaccggttg caggtgttgc catgggactt atgaaagaag
    89461 gagacaatgt agttgtactt acagacattc aaggcatgga agatttcctt ggagatatgg
    89521 actttaaggt tgcagggaca aaagatggaa ttacagcaat tcaaatggat ataaaagtcc
    89581 atggaattga caaggaagtt cttacaaatg cacttgaaaa agctcataag ggaagaatgt
    89641 tcatattgga taagatgctt gaatgcatag ataagccaag agaagaagtt agcaagtacg
    89701 cacccaagat tatcactatg atgattgacc ctgataaaat aagagacgta ataggttccg
    89761 gcggaaaagt aattaacaag attattgaag aaacaggggt aaaaattgat attgaagatg
    89821 acggatcagt ttcaatagca gctgaaaatg ttgaaagtgc atacaaggcc aagagaataa
    89881 ttgaaagtat agttaaggaa atcgaagtag gagagattat tctgggcaag gttgtaagaa
    89941 tagaatccta tggagccttt ataaagcttt ctgacaacac ggacggtctg cttcatattt
    90001 cacaaataga cagcaaaagg atagaaaaag ttgaagatgt cctgaaaatg ggtgatgagg
    90061 tgctggtgaa ggtaatcggc atagacgaaa agggtaaaat caagctttca agaaaggcag
    90121 caatgcagca gaaagaaaat taaaaactgc ttattaaatc agtgttaaat aaaatcgagg
    90181 caaagacctt gattttttta atgcttaatt atatgttgta attaatgcaa aaatttgata
    90241 atataaaact agatatatat cataaacgca cccttttatt aatacattta agataagggg
    90301 gatttttatg aaattttttt gcaattccgg attcattaga aaagcaataa tagtaacact
    90361 attatcgatt attatattat tgttattaat gggcagattc aggaccatga gaataagaat
    90421 tcttggattg gcggaagggg gatacatctg cagaactgca gagacatacc tgcaaagtca
    90481 tggggacgca cccatgctgc tatggggata gtcctgccag tcagaggaat gaccgcaaat
    90541 tataagtctc tgtcccataa tttactggag gaaaaatgat aaaaaagcac acattggata
    90601 acggaatcag aatagtaatg gaaaaaatag actatgtaag gtctgcgtcc ataggcattt
    90661 gggttaatgt gggctcaaat aacgaaaatg atgaaactaa tggtttatca cactttattg
    90721 agcacatgct tttcaaagga acaaaaaaca ggaaagcaaa tgaaattgca gaggaaattg
    90781 acagtatagg cggtcagctg aatgcattca cgagcaagga atgcacctgc ttttacatca
    90841 aggttttaga cgaaaatata aaagaagcag tagatatact ttcagatatg ttttttaact
    90901 ctgtattttc acaggatgag atagataaag aaatatctgt tgttattgaa gaaataaaaa
    90961 tgtatgaaga ttctccggag gatattgtac atgataaatt atctgagatt atttttaacg
    91021 aaagccccat ggcatataat attctcggaa cggaaaaaaa ccttaaaact ttcactcatg
    91081 ataaagtaaa aagctttatg gaagaaaatt attctccttc aaataccgta atttcaattg
    91141 ccggaaattt cgatgaagaa aaattcatag aactgataga gactgaattc aagtcctggg
    91201 aaaataaaaa agtaagaaaa gttcacaagg aatcaaatta tcaaagaaag gttgccggaa
    91261 taaataagga cttggaacag cttcatatgt gcataggcaa caagacattc ggaagacatg
    91321 atgagaacta tcattctctt ctggtattaa acaatttgtt cggcggaaca atgagttcaa
    91381 gaattttcca ggaagtaaga gaaaaaaaag gtcttgtata ttctatttat tcatttgtat
    91441 ccaattattc cgatacagga attttcacaa tatatgcagg catgagctac gagcaggctg
    91501 aagatgccct gagaaccata ttgaaggaaa tggtaagcat aaaaaacgga aatattaaag
    91561 aagaagaatt cctgagagca aaacagcaga ttaaaggaaa ttatatttta agtcttgaaa
    91621 gcacatcaag cagaatgtct gcaataggca gaagagaact tttatataat gaaattttat
    91681 atcctgatga ggtaattgaa gctatcaaca atgttaaaat tgaagatgtc ctggatgttg
    91741 caaaggacct gttcaacatc gataatttga gcataacttt tacagggaat ttgaaaaaac
    91801 atcctgatct tgagggtaaa ataaataagg tactgggaga aaaatatgat aattaaaata
    91861 gttaagaaaa atccttttaa attaccgtgc tatgaaacga aagggtctgc cggagtagat
    91921 attcaagcct ttgttgaggc acccatggta ttaaaaccaa tggaaagggc actggtgcct
    91981 acaggtttat ttattgaact tcctgaaggc tatgaagctc aaataagggc cagaagcggt
    92041 ttggcaatca aacacggcat aagtctcgta aacggcatag gaaccataga ctctgattac
    92101 aggggagaaa taaaggtaat tttaattaat cttggaagcg aggattttac catcaacagc
    92161 ggggacagga ttgcccagat ggtggtaatg aagcatgaac aggctaaatt tgaattagtt
    92221 gaagacttaa atgaaacgga aagaggtcag ggaggtttcg gacatacagg aattcattga
    92281 tagttgatag ggggggaata tatgaaatta agcgaattgt acagcaagga aatcattaat
    92341 gtggacacag gggaaaactt ggggattttc ggtgactgcg acctggttat agatgaaaaa
    92401 acaggggaaa taatcagctt tatatcagga aacaattcac attttgcatt tttcaaagaa
    92461 ccgaaaagaa aagaaatact atggaaaaat atcaaaaaaa tcggaagcga tatgatcatc
    92521 attgaaaatg aataaagatg acctttacga cctaacccaa gacttcgact aaaaggagaa
    92581 agtcttgttg gtaggtcaaa cgcacaaaat tactaattta tgctacgaaa ggagcaaata
    92641 aattagtgaa tttacctggg aaccatattt taaaatatag tatttaactg aagatagcac
    92701 atctattgtt ttagaataat gagaaaaatg tataaaatta ataaaactcc aaagaataat
    92761 caggaatacc tgattatttt tttatggagg agtaatggaa aacaagagca ctaattcaca
    92821 gcagcagcca cagacatcat ttaacaattt ggacggacaa agaaacaata agcaaaatca
    92881 attccgagac gagccggtaa atccgtttat attcagcggc acaccaagcg aaaatgaaga
    92941 cagcagcgag cctatagaaa atgttaaaca agtaggaagc ataactgaag ttgagccggt
    93001 aaaggatgtt tgttttctaa gtataatagg agaaattgaa gggcatacct cactgcctcc
    93061 ccaaagcaaa acaacaaaat acgagcatat aatacctctg attataggag ctgaaatgga
    93121 cccccagata aaaggggtgc ttttattgat aaatactatg ggaggagacg tagaggcagg
    93181 cctcgctatt tcagaactgg tagcaagtat tacaaagcca actgtttccc ttgtattggg
    93241 aggaggtcac agcataggaa cggcactggc tgtttcagcc cggtactcgt ttattgcacc
    93301 cacaggaaca atgacaattc atccaatacg aaccagcggt ctggttatag gagttcctca
    93361 gaccttcaga tatttccaaa aaatgcagga gaggattaca aattttatcg taatgaattc
    93421 acatgttgac aggcaggcgc ttttggatta tatgtatgac acagatgaaa tagccaatga
    93481 tgtgggaaca gtattaaatg ccgaagaagt tgtcaatata ggcttaatag aagaaatagg
    93541 cgggttcagc tctgctatga aaaagctcag agagctgatt gatgatatgg gaaaataaaa
    93601 gggaagtcag ttaatgacaa aggttgacaa actttatttc attattattc agagggagaa
    93661 gtctagttta cgaatttgaa attatcattg cgaaatgttc tcatcctatg atataattaa
    93721 atttacaaag aaacaggggg aaatgcttta aatggctaaa acatccgcaa acaaaacgac
    93781 caggacaaac aaaaaaacaa atacaaacaa aaaaggtaca agcaccaggg cttcaggcaa
    93841 ggatatttta attaaagaga ttaccggtat cttccttgta tcttttgctg tattcatgat
    93901 tatagttact acaggagaat ctcaaacagg aattatcggc agaagcataa actcatttac
    93961 taattcagca tttggggcag gctctgaagt acttccttat tttttacttg caattggggt
    94021 tacaaggctt ttaaacatag tcatattgaa tgacaacaat caaatagccg caattatagg
    94081 attctttatc tgtttcatca tatacagggc agtatcagac ccggaagttt attcgcaaat
    94141 agccttaacg aaagacttgc aggaattgtt tataatatca aaagatctgg ggatggcagg
    94201 caaaggcggg ggtatttccg gaaatttaat ttcatctata tttgttaaac ttatcggtaa
    94261 aaatggaaca tacatagcct tgggagcatt tatcttttca ttgattgttc ttcttacaaa
    94321 tcaaagtata atcggtattt taaaattaat atcaaattac ataaaaaagt tctttaatgt
    94381 catatataaa ttcatattta cggaagacgt tgaaaataaa agaaaaaaag aaaaattacc
    94441 aaaacagcag ccaaatgaaa atataaaaat ttatgattac atgagtcctg cacaagataa
    94501 taaaaaagct gaaataactc agttaaaaat agaagatata caaaaagcag agaacaaaac
    94561 tgataatagg acagaaacac aaaaggacga accccttccg gatgcaaagg aaaaattcga
    94621 gaaaaaggta attactgatg acataattac catatcagat gttaaagcag acaattacaa
    94681 gctgccttca atcaggcatt taaaccccat gcctcttaac gtaagaaatg acaaggaaga
    94741 cttgaaaaag gacggggaaa agcttattga cacattaaac agcttcaaca taccttgcaa
    94801 catccttcag attaataagg ggcctaccat aaccaggtat gaaattcagc ctgcacctgg
    94861 aataaaagta agccggataa cagccttgtc caacgatata gccttggcac tggccagctc
    94921 ggaaataaga atggaagcac ctatacccgg caaagccgca attggcatag aggtgccaaa
    94981 cagggcaaaa accagcgttt atttaagaaa catgattgaa tcaagagagt acagaattgc
    95041 tcccacaaac ctgccctttg cacttggcaa ggatatagca ggaaaaaata ttattgcaag
    95101 cattgataaa atgcctcacc tgttaatagc aggcgctacc ggctccggaa aaagcgtatg
    95161 tataaactcc cttattatga gtattttata caaggcaaaa cccgatgaag tcaagctgat
    95221 actcattgac cctaaggttg ttgaactgag caactacaac ggtatacccc atttattgat
    95281 acctgttgta actgacccca gaaaggcagc caatgcgctg aactgggctg tgtcggaaat
    95341 gacgaaccgc tacaaggttt ttgcacagaa cagcgtgcgg gaaatagact cctacaatga
    95401 aaaaatggct gtggaaaaca aggagaaaat gcctaaaatc gtaataataa tagacgaatt
    95461 ggcggatttg atgactgtag cctcggcaga ggttgaagaa tatattacac gtattgccca
    95521 gctggcaaga gcctgcggta tgcaccttgt cattgcaacc caaagacctt ccgttgatgt
    95581 tattacaggt gtaataaagg caaatatccc gtcacgtatt gcatttgccg tgtcatcaca
    95641 tatagattca agaactattt tggatacagg gggagcagag aagcttcttg gcaaaggaga
    95701 tatgctttat caccccatag gtcttccaaa acctgtaaga atacagggaa cctttgtatc
    95761 agatgatgaa atcaaaagag ttgtagactt tatcaaggaa cagcaggttg aaatcaaaca
    95821 caacaaacaa aatgaaataa tcaaagaaat tgaaagccag accggcgacg atgatgatgt
    95881 tgatgaatac ttggaacagg caattgaaac agtggtaaat gacggccagg catcggtatc
    95941 ctacattcaa agaaaattca gggtaggtta ttcaagagct gcgcgaatta ttgaccagct
    96001 tgaggaaagg ggcatagtag gagggcatga aggcagcaaa cccaggaaag ttcttgttac
    96061 aaaagaggaa ttagacgaaa tgagaggaaa ttcataaaga atgaaaaaaa tatacatgca
    96121 ttcgcttgga tgctcaaaaa atcttgttga ctcggaaaat atgcttggca ttctaaccga
    96181 aaaaggatac aagacaacgg attatgcgga caaggcagac tacataataa taaacacctg
    96241 ctccttcata aatgacgccc aggaagaatc agtaaatgcg atactttccg cagctgatat
    96301 aaaaaacacc ttaaacaaaa aaacaaaaat aatagtgaca ggctgcctgt ccgaaagatt
    96361 cggggcagag ctgggaaaag aaatacctga agttgacatc atagtaggaa cgggcggttt
    96421 tgaaaaaata gaccaatata ttcaagagtt tgaaaagaat aaaaagcttg tgatggacac
    96481 aggatctgag cttgatatcg acaaggacct tccgagaaac atattgactg aaccctggta
    96541 cgcctattta aaaatagctg aaggctgcag caataattgc acctattgcg tgattccgaa
    96601 gctcagaggg ccttttaaaa gccgcataat cgagaatatt gttgaggaag caaaggtcct
    96661 ttcagaaaga ggagcaaagg aaataattat cattgcacag gacacaagca agtacggtct
    96721 tgatatttac ggcgaaagaa aacttcatga acttgtagga agaatttctg aaattaaagg
    96781 catcaaatgg ataagagttc attatttgta tcccgaagat atttacgatg aactgataga
    96841 ggaattcaaa aacaataaaa agcttttaaa atattttgat attccaatgc agcatataaa
    96901 tgacagaata ctgaagagaa tgaatagaaa cacaaaccgg gaacaaatta catccctgat
    96961 tgataaaata agaagggaag tggagggtgc ggtaatacgc acgtccttaa tcacaggctt
    97021 ccccggggaa acatgggaag accatgaaga actcaagaat tttttgaaag aatacaggct
    97081 tgacagggta gggatattca aatattcaag agaagagcat actccggcat acaggctgcc
    97141 ggaacaaata gatgaagaca taaaggaaga aagattaaaa gagttgatgg aaattcagca
    97201 agcaatatca tatgaaaaca atttgctgaa aatcgacagg acctatgatg ttttaattga
    97261 ggagaaggaa gatgaacata tctatgcagg caggacctac atggattcga ttgaaattga
    97321 cggctgtgtt tatgtcacat ctgatgaaga attaacctta ggaaacattt ataaggtcaa
    97381 aattaatgac gcattggaat atgatttgat aggaggtata gaagatgaaa ataataaatt
    97441 ggtttaaaga tttaaattta cccaacaagc ttacagttat acgggtattg gcaatacctt
    97501 tctttctgtt gttcttgtac ataagcggcg gaattttcag gtttctgccc ctgttaatct
    97561 ttgtgggtgc tgctgttacc gatgcctttg acggacatat tgcaagaagg gacaaattag
    97621 taacagattt cggaaaattc acagaccctc ttgcagacaa acttctgaca gcatcggcat
    97681 tcatagcttt tgtagagctt ggctatatca gcgcatggat tgttgtactg attatatcaa
    97741 gggaatttat agtttcaggc tttaggacgc tggctgcttc aaagggtgtt aaaattgctg
    97801 caaatgcatg gggaaaaata aaaacagtgt tccagatggt attaattatt gttattttat
    97861 tgaactatac tggttatttt ggttttacag attttttaat aactccttta atcgcaatta
    97921 cagtattgct gaccgttact tcaggagcag tttatattta tgaaaatctt ggtgttataa
    97981 aaaattagtc attgacaata ctttttattt tatgtataat caaaggtgaa caaatgttct
    98041 gataaggagt cggatatgga aaaagataaa gcacttgagc ttgcccttgc tcaaattgaa
    98101 aagcaattcg gtaaaggttc tataatgaag cttggagaaa atgcaaaatt aaatatagaa
    98161 gccataccaa ccggcgcact gcccctcgat attgcaaccg gaatcggcgg agtgccaaaa
    98221 ggaagaatca ttgaaatatt tggacctgaa tcctctggca aaacaacagt tgcattacat
    98281 ataatagcag aatcgcagaa aagaggcggc atagcagcct tcattgacgc agagcatgcc
    98341 ctggacccgg cctatgcaaa aaatttaggc atcaaggtgg aagagcttat aatttcacag
    98401 cctgataccg gggagcaggc tcttgaaata gcggaagcac ttgtaagaag cggtgccatt
    98461 gatgttatag ttgttgactc tgtagccgca ttggtgccga aagctgaaat agacggagaa
    98521 atgggggatt cccacatggg gctccaggca aggctgatgt cccaggcctt gagaaaactg
    98581 gcaggagcta taagcaaatc aaacacggta gctattttta taaaccagct ccgtgaaaaa
    98641 ataggcataa tgtttggcaa ccctgaaacc acaaccggcg gtcgggcttt aaaattctat
    98701 gctacaatga gacttgacgt aagaagaaca gaaacattga aaaagggcga tgacgtatac
    98761 ggcaacagaa caagagtcaa ggttgtaaaa aataaagttg cccctccctt taaacaggct
    98821 gaatttgata taatctacgg caaaggtata tcaaaggaag gctgcatcct tgatatggca
    98881 gtggaagcag gagtggtaaa caagtcagga gcatggtact catacgaaaa caaccgaatt
    98941 ggacaaggca gggaaaattc taaggattat ctgttgtcaa atcctgaact tatggctgaa
    99001 atagagaaaa aggtactgga aaagtatgaa ataggagctt tgaaactgtc atcagacgac
    99061 agggaagaca catcagtaga tgactaaatg tcatatgtca ttcaaaagtt aatgtcatta
    99121 ttaaaaaaca taattaaggc acccgcaagg tgccttttta tcatatcgat atatagcaca
    99181 gcctatgtaa aggggtttac ataatttaaa ttctgtaaac caacgtgtca ataaactata
    99241 tgaatataca aattatacaa attatacaaa tataaacata tatcttgaca ttatttataa
    99301 ataattataa aatatagtat attcaagttt tatagaaact tattaaaacg attaatataa
    99361 atatgttttg gaagaataca cattttttaa tgaatcaaga aaaatgtata tatttcgcag
    99421 cttgaaaatt taatatggga ggtgctgaat tgttcgaaga aatattagta ggtattatct
    99481 gcgcaataat aggtataatt ataggatttt ttattcgtaa aaatatatct gaaggcaaaa
    99541 taggtagtgc tgaaatagaa gctaaaagga taattgaaga cgcaaatcaa aaagctgaaa
    99601 caaggaaaaa agaaatactt gttgaagcaa aagacgaagc tcacaaactt agaaatgaat
    99661 atgaaagaga aaacaaagaa agacgaaacg aaattcaaag aaatgaaaaa aggcttatta
    99721 aaaaagagga aatgctggat tctaaaagcc ttgtagtaga aaaaaaggat gaaatgcttc
    99781 aaaagaagct gaaagaagtt gaaacaaagc aagaaaaatt agaggcaatt caccagaaac
    99841 aaattgagga actggagaaa atatccgggc tttccacgga agaagcaaaa gatattctta
    99901 taaacaacat aaaaagagaa gccgaacagg aagcagcagt agctgttaaa gaaatcgaaa
    99961 gggaatcaaa agaaactgca gaaagaaaag cacgtgaaat tattacatat gcaattcaaa
   100021 aatgttctgc agaccatgta gcagaaacaa ctgtatctgt agtggacctg cccaatgatg
   100081 aaatgaaagg cagaatcatc ggcagggagg gcagaaacat aagggccttg gaacagctta
   100141 caggtataga tattataata gatgatacac cggaagcagt ggttatatcc ggatttgacc
   100201 ctattagaag agaagtggcc agattagcgc tcgaaaaact gattgcagat ggaagaattc
   100261 atcctgcaag gattgaagag atggtagata aggccaaaaa agaagttgat caaataataa
   100321 aggatgaagg tgaacaggcg acccttgata ccggaatcca cgggcttcac ccggaattgg
   100381 ttaaattact cggaagattg aagttcagaa caagctacgg ccagaatgta ttaaaacact
   100441 ccattgaagt atcatattta gcaggaatta tggctgctga actgggcgct gacgtaaaag
   100501 tagcaaaacg ggcaggcctc ttgcatgata taggaaaagc agtggatcat gaggtagaag
   100561 gtcctcacgt tcaaataggt gaagacctgg ctaaaaaata caaggaaagc aaggctgtaa
   100621 ttcatggtat agcagcccat cacggggata tagaaccgca aaccgtagaa gcagtattaa
   100681 tacaggcagc agatgccata tccgctgcaa gaccgggagc aagaagagaa actttggaga
   100741 cctatattaa gaggctggaa aaactggaag agatttcaaa ttcatttgaa ggcgttgaaa
   100801 agtcttttgc aatacaggca ggaagagaag ttcgaatatt ggtaaaacct gaagcaacaa
   100861 gtgattcaag cataatttta atagcaaaag atattgttaa aaagattgaa gaagaaatgg
   100921 aatacccggg acagattaaa gttaacgtaa taagagaaat cagagctaca gaatatgcaa
   100981 aataagagta agaaaaatat ggaagtaaac gacacgttta cttcttttta atatatgatg
   101041 cggaaacgga ggtaccataa catgacaaac aggaccaata acaatacggt gacgctgtat
   101101 aacaacaatg gacacatgaa aagaggattg accttgtcat cattatatat tagaaaataa
   101161 ttgtttataa aaggaggaaa aaatgaatta taatccggca gaatataaat acccgtcaag
   101221 aagaaacgta atttacggaa ataacggaat ggttgccaca tcacaaccct tagcggcaca
   101281 ggcaggtctt gaaatactta aaaaaggagg caatgccgtt gatgcagcca tagcgactgc
   101341 agcatgcctt acggttgttg agcctacatc aaacggcctg ggaagcgatg cattcgccat
   101401 tgtttggttt aataacaggc ttcagggatt aaatgccagc ggttattcac cttacagcat
   101461 ttccatagaa gaattaagag aaagaggata tacagaaata ccaaaatacg gtgtcattcc
   101521 ggtgatggtt ccgggagctc ccgcagcttg ggttgaacta aatgataaat ttggagccct
   101581 tcccctgata gaagtgctga agccagcaat taactatgca aggaatggat ttccggtatc
   101641 tgttaccgta aaaaaagcat gggatgcagc gtctgagatt tacaaagaag aaaataagga
   101701 agaattaaaa tactggtttg atacatttac gaaagatggc agaactccaa ggtttggaga
   101761 gcttgttaaa ctgccgtttc atgccggaac attggaatca ataggcagaa ccaacggcag
   101821 ctcttattat aaagggcata ttgccgacaa aatagatgca ttttttaaaa aatacaaggg
   101881 ttatttaaca aaggaagaca tggaaaacta taaacctgag tgggtaaacc ctataagtgt
   101941 aaattacaga ggctatgatg tgtttgaaat accgcccaac ggtcagggat tagttgctct
   102001 gatggctctt ggcatattga aaaactttga ttttacattt aaggaaaaca gcgataccta
   102061 tcacagacaa attgaagcaa taaagcttgg ttttgctgat ggacaaaagt atataacaga
   102121 ttcaaggcat atgaaaatag atataaagca gttattgtct gatgaatatg gcagaattaa
   102181 gtcaaattca ataaaggaaa aggcattgat gccgaaagaa attgactcca accgcagcgg
   102241 aactgtttat ctttgcactg cagacaaaga cgggaatatg gtttcttata ttcagagcaa
   102301 ttacatggga ttcggctcag gcgtagtaat tcctgagacc ggaatagcgc ttcagaacag
   102361 gctgcataat tttaattttg acaatgacca tagcaattgt ttgatgcccc ataagaaaac
   102421 ctaccataca attattcccg gatttttgat gaaggatgga aaagcacttg gacccttcgg
   102481 tgtaatgggg ggatttatgc agcctcaagg acatgtgcag gttgtgacca atatgataga
   102541 cttccatatg aatcctcagg aagctataga tgcgccaagg tggcagtgga taaatggaat
   102601 taacgttgaa gtggaaaagg atgtgcctgt caacatagta agggacctgg agaatagagg
   102661 tcataaagtt acaatacagc caaatgaagc tctttttggc agaggccaaa tgatattgag
   102721 aacaaatgaa ggcacactat gcgggggaac agaaaagaga gctgacggac atatagcagt
   102781 atattaattt acataattta aattatgtaa atcaatattc aaaaacaaag aggctttgat
   102841 attttatagc ctcgtttttt ttataatgaa taagttttcc aattaaggtt aaaattctaa
   102901 ttgaacaatt attttagccg ggaggaaagc aaatgaaaaa tgataaggat gctcagttaa
   102961 atcaattta
//
LOCUS       DAOPUT010000002        57096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_500_length_57096_cov_18.953770, whole genome shotgun sequence.
ACCESSION   DAOPUT010000002 DAOPUT010000000
VERSION     DAOPUT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 57096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57096
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_500_length_57096_cov_18.953770"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1935)
                     /locus_tag="PKK75_00500"
     CDS             complement(13..1935)
                     /locus_tag="PKK75_00500"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNZ82101.1"
                     /translation="MDKNFEYIRVASAVPSLKVADPDYNVKEIIKICLQAAKKGVRVL
                     VFPELSISGYSCGDLFYQNTLINECEEALNLLIEETRYLNMLIAVGMPVKADARLYNC
                     AVLLKEGKILGIIPKTYIPNYCEFYEMRWFAPAESRMWETIVMCDQEALFNENLLFKD
                     NISSLCIGVDMGEELGANIPPSSYHTLQGANIILNLAASTETAGKADYRRELVRIQSS
                     RCITSYVYSSAGEEESTTDSVYSGHCLIAENGRILKEDNLCGGFIYSDIDIEKISNER
                     ISRNNNSHIKKDYKFVNFNLGWSENLTLERFIDPRPFVPSNPENRDRRAGNIINIQAA
                     GLSQRMKKINSKKAVIGISGGLDSTLALIVTIEAFKKLRLPLTNIISVIMPGFGTSDR
                     TSGNAHVLTAELGTSLREISIEDACIQHFKDISHDIENHDVTYENAQARERTQILMDI
                     ANQEGGIVIGTGDLSELALGWCTYNGDHMSMYSVNSGVPKTLVKYLVKWYADNKTTGK
                     MQKTLLDICDTPVSPELLPPDKEGNIRQLTENSVGSYELNDFFLYNMLGNGYGPEKIY
                     YLANIAFKDDYDPETILSTLKKFYKRFFTQQFKRSCMPDGVKVGSVSLSPRSGWKMPS
                     DASFDLWIKKCENIRK"
     gene            complement(1944..2717)
                     /locus_tag="PKK75_00505"
     CDS             complement(1944..2717)
                     /locus_tag="PKK75_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNZ82102.1"
                     /translation="MNVSSIINFLNDEVKLKDHESWDNSGLQIGSMEVNINKIMLTFD
                     LDMEAAKHAVKEKADLIITHHPFFFSSIKKIDRDTHEGQIIYMLIKENINLYSIHTSY
                     DMADKGVTYNLAEKLNISDYQVLHPVNIDNSGYGGIGEVAPINIREYSKFVKEALKAD
                     YIKLYCNDEKTVKKVAFCGGSGSEFIQDAIRQGADVYITGDIKYHQAQEALKNNLCII
                     DGGHYNTEVHSMELIEEVLKKTNIQIINFKRNMTLEKII"
     gene            complement(2714..3388)
                     /locus_tag="PKK75_00510"
     CDS             complement(2714..3388)
                     /locus_tag="PKK75_00510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ82103.1"
                     /translation="MNRLECIVSMVDNCRVAADIGTDHGYVAEMLLRERKCDKIIAAD
                     LNGGPLKRAIEHLSSVKMNDRCDFRLGNGLTVLKEGEADAIIIAGMGGELISFILETS
                     KDIALNADQLILQPMTAADDLRRYLAENGFEIIDEKIVREGYHFYFVIKAKSGTAKYK
                     DSLYYEISKNLIDKKDPVMIEYVNKVLNTNKKIIRSLEKAGNVEYNVKIEFLRDKNEK
                     IKELIK"
     gene            complement(3670..4839)
                     /gene="rpoD"
                     /locus_tag="PKK75_00515"
     CDS             complement(3670..4839)
                     /gene="rpoD"
                     /locus_tag="PKK75_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNZ82104.1"
                     /translation="MNDNKNISVISEEELVKNAEMLQKIDSIKNKLIEKGKRNGFITY
                     KEVLRSFERLDINPEFIDDFYKQAEDNDLEILGFQDDMIPEKVDEEDEADVKDDADFF
                     VSQSDEDILKGVNIDDPVRMYLKEIGKVPLLTALEEVELAKKMEDGDEEAKKQLAEAN
                     LRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGFKFSTYATWWIRQAITR
                     AIADQARTIRIPVHMVETINKLIRIKRQLLQELGRDPTPEEIALEMEMEPDKVREILK
                     IAQEPVSLETPIGEEEDSHLGDFIPDDEVQAPSDVATFTLLREQLSNVLHTLTDREQK
                     VLRLRFGLDDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLKDYLE"
     gene            complement(4842..6596)
                     /gene="dnaG"
                     /locus_tag="PKK75_00520"
     CDS             complement(4842..6596)
                     /gene="dnaG"
                     /locus_tag="PKK75_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNZ82105.1"
                     /translation="MPYRLDSDVIQRILDQNNIVDIIEEYLPLKKAGANYSTNCPFHK
                     EKTPSFIVSPDKQIFHCFGCGESGDSIGFLTKYRNFTFVEAAEYLADRAGIVLEEINS
                     PRINNNKETADKLYRINRDAAAYFYKNLRNYPHVIEYLRSRNIKSNVIKLFGIGYAMG
                     EWDNLLNHLKNEGYKEEDILKAGLIIRNEKNDTYYDRFRNRVIFPIFDVKKRIIGFGG
                     RVLDDSLPKYLNSPESLVFNKGYNLYGIHIAKENAKDNSFILVEGYMDVIKMHVHGYS
                     TAVAALGTSFTENQVKLLKRYSKNFYIAFDSDEAGQKASLKALNLLKKNNLKAKVLIM
                     KDAKDPDEYLNKFGKSGFDKLIENSLDYYSFLEFHFKEILENSNKVEYINNFFDNIVN
                     VNSEIERELIFEKLSRKAGVSKESIINEYNKRSTKQKTLVKAAKPVIKPQVKKITTCH
                     EEELIKLILLNNDFALKLSEIVKEDTFKDLKFYKLFKELYECRINDTGIDSESLYKIT
                     NGSMDVNMQIDNVDYDNLEALFKDCMKRLKLKYYEEKRNILTNSLKQSDNSLNSKEIM
                     NEIFCLAKKIKSTKEEVN"
     gene            complement(6727..7728)
                     /locus_tag="PKK75_00525"
     CDS             complement(6727..7728)
                     /locus_tag="PKK75_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNZ82106.1"
                     /translation="MNIREKYEQFEIDHLSEYAAKALHSKGRRTDEPKCEIRTDFQRD
                     RDRILHSKSFRRLKHKTQVFLSPEGDHYRTRLTHTLEVSQISRTIARSLNLNEDLTEA
                     IALGHDLGHTPFGHTGERILKKLCNGFKHNEQSLRVVDFLEIRNDKPGLNLTFEVRDG
                     ILNHPSGCEPATLEGKIVSISDRIAYINHDIDDAVRANIITKIPDKFIIKLGSSHGRR
                     INTMIMDIIHNSADKNNIVMSDEVAELTKELRDFLFENVYYNKIAKSEDEKIMFIVEK
                     IYEYYTKNFDALPEFYLNIYNNNSFSREEIIKDYIAGMTDRYAMKVFDDLYIPKPWL"
     gene            complement(7897..9291)
                     /locus_tag="PKK75_00530"
     CDS             complement(7897..9291)
                     /locus_tag="PKK75_00530"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNZ82107.1"
                     /translation="MAKTEKNSLEKLVSLSKSRGIIFPGSEIYGGLANTWDYGPLGVE
                     IKNNVKRAWWKKFIQESPLNVGLDAAILMNPTTWVASGHVGGFSDPLMDCRECKARFR
                     ADKLIEDYMKEAGDVKIVDGWTNNEMEAYVSENKVKCPECGAQNFTPIRKFNLMFKTF
                     QGVTEDSQSELYLRPETAQGIFVNFKNVQRSMRKRIPFGIGQIGKSFRNEITPGNFTF
                     RTREFEQMELEFFCKPGEDMEWFYYWKEFCMNWLLSLGISKEKLRFRDHEPAELSHYS
                     KGTSDIEYLFHFGWGELWGIADRTDYDLKQHINTSGEDLYYVDPVTNEKYIPYCVEPS
                     VGVDRVMLAILVDAYNEEILEDGTDRVVLKLHPALAPFKAAVFPLTKKLNEQAEELYK
                     TLSKHFNIDYDDGGSIGKRYRRHDEIGTPYCITFDFDSLEDNTVTVRDRDTMEQVRIK
                     IDDLVTFINEKIKF"
     assembly_gap    9750..9849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9850..10373)
                     /locus_tag="PKK75_00535"
     CDS             complement(<9850..10373)
                     /locus_tag="PKK75_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNZ82108.1"
                     /translation="MENDNLQKIDEILRRTNTDYATAKEALDAANGDVLEAIILIEGR
                     QKGGRSGSNRGEQIMSQLKDILAKGNATKLTIKKNNEVIINIPITAGLVGAFIAPFLS
                     AAGITVALLAKCSVEITQTDGKVIDIGQKVDEGMDAVKDAVQDLKSGAKNMRDDLKTE
                     AENMKDDIRTGAEN"
     gene            complement(10388..11101)
                     /gene="recO"
                     /locus_tag="PKK75_00540"
     CDS             complement(10388..11101)
                     /gene="recO"
                     /locus_tag="PKK75_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNZ82109.1"
                     /translation="MIEEEILVLKEISYKENDKILHALSKTRGKIQIISKGSKKSSSH
                     LINASQIFAYSSCTLNPSKDMYILTSARLVDNFYNLRNNMDAYYNACYILELISYVAQ
                     ENEYDMRVFDMTVSVLSHLSNFTKDFDKLTAAYELKLAAMLGYKPDFLHCLHCGSSIN
                     ENARFSITEGGVFCSDCVNFGNGINVTYNEILIMDKILKSKFENIGFIEVTRHIMNLI
                     RKFLFYYIGRDNFTSLKLL"
     gene            complement(11098..11985)
                     /gene="era"
                     /locus_tag="PKK75_00545"
     CDS             complement(11098..11985)
                     /gene="era"
                     /locus_tag="PKK75_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNZ82110.1"
                     /translation="MFKSGFVTIIGRPNVGKSTLMNNLIGEKISIMSDKPQTTRNKIM
                     TVYTDDEAQIIFMDTPGIHKPKNQLGEFMNTEVDSALEEMDVLIMITDEFNKVGPGDE
                     FIIEKIRGAKAKKILIINKIDKFDQEAALRIAAEFDKYKVFDDIIPMSALLNTGVDAL
                     LKLIVKYLKPGPMYFPEDFITDRPERFVVSEIIREKALMYLEDEVPHGIAVEIEEMKE
                     RKNRNLVDIRANILCEKKSHKSIIIGKDGRKIKGIGKSAREDIERLLGSQVNLQLFVK
                     ISENWRDDNYRLKILGYDK"
     gene            complement(11998..12390)
                     /gene="cdd"
                     /locus_tag="PKK75_00550"
     CDS             complement(11998..12390)
                     /gene="cdd"
                     /locus_tag="PKK75_00550"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNZ82111.1"
                     /translation="MEDKLLIKIAMEAREKSYSPYSKYKVGAAVYTRSGKVFTGCNIE
                     SASFTPTICAERVAVSKCISEGYRDIDKIAVVGSEIKTSFPCGVCRQFMSEFGRDIKV
                     ICARNVDFYSVYTLEELLPNSFGPDDLD"
     gene            complement(12475..13548)
                     /locus_tag="PKK75_00555"
     CDS             complement(12475..13548)
                     /locus_tag="PKK75_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNZ82112.1"
                     /translation="MKKITLLILFLLILSGCTRETVTPEPENIEIVEEEPEEPVITEE
                     EPEVEEEEEEIDPDKVLSPLDGLRYYPEDLLKRPVALSIDNHPRARWQAGISQAEIVF
                     EVEVEHPFTRYLCIFLAKEPERIGPVRSARPYLIYYALEYDGIFVHVGGSEDAFQKIK
                     SLGVADVDGLYSGAMWRYNDTGKYAPHNVYTTLESIRNEAKKYGYRTEAEFEGYTFNE
                     NDGKLSENFEISTARKVNIVYNDYNTTDYIYDDEKGLYLRYKDKEEHIDELDKKQITA
                     KNIIAIETSKSVLDNEGRLYLGTIGEGKGIYITNGESIPVTWEKTTEKDRLKFYAGDK
                     ELVLNPGNTWIQVVNSLENLNLE"
     gene            complement(13566..14012)
                     /gene="ybeY"
                     /locus_tag="PKK75_00560"
     CDS             complement(13566..14012)
                     /gene="ybeY"
                     /locus_tag="PKK75_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNZ82113.1"
                     /translation="MISVLFDDRQDMMEINGDNEAAIERAVEAVLTEEECDGDFEISV
                     SFVTNEEIKELNKEYRNVDSETDVLSFPMNEEFDGVTILGDIVISTQKIIEQADDFGH
                     SPEREMIYLTVHSMLHLLGYDHMENDEKSAMRAKEKEIMKNLKIFK"
     gene            complement(14009..14974)
                     /locus_tag="PKK75_00565"
     CDS             complement(14009..14974)
                     /locus_tag="PKK75_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNZ82114.1"
                     /translation="MDLFEQNILIANEDELIKLLGIHDKNIKIIKSYYDVNILVRNGA
                     LKVTGEKESAEAAAKIIKDILNTIRAEGEISDQKVVYLIEANKSNSQIDYNQILKEVI
                     VTSASGRIIKPKTVGQKRYVDMINQKDVTFGIGPAGTGKTYLAVACAVNAFRKKEVER
                     IILTRPAVEAGEKLGFLPGDLQDKVDPYLRPLYDALFDIMGIDNYMKNVEKQIIEVAP
                     LAYMRGRTLDSSFIILDEAQNTTNEQMKMFLTRLGYGSKAVITGDITQIDLPEGKKSG
                     LKSVIHILKEVEDIGFMFFHTTDVVRHKLVQDIVNAYEKHEGQNK"
     gene            complement(14974..16179)
                     /locus_tag="PKK75_00570"
     CDS             complement(14974..16179)
                     /locus_tag="PKK75_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HNZ82115.1"
                     /translation="MLIFKLMYLIRGYVVIRRDDMNSEKLLNILRRRNISVWDVEKKD
                     KKIKFKISYEDYIKHSDLINEIVKPESKRGVLLKLNKLKLRRGFSIGLLILIISLYLL
                     TSMIWDVEVIGTNQPNTNEIIRLLEKNKIKLPFALSQIETKKLETLIYDNFQFKFVEV
                     FVEGSKLIIFVREREPEPAEIKSNEPSSIIALKNAIVNKVIAKSGQAVVKEGDVVYEG
                     QTLIMGIVKNKNSEEFMMVPSEGTVYGKTYYNFEMKEEKIRNVNVSTNKSNAVYYLII
                     NGNSIKIIGDKDPYENYNYREREINVPLVSNIADISILKGVYYEEVIKKIEIDKETAH
                     NKMKVSMYDELLKSCDEDSRILKSNINFSEDEEFYYLIAQIEVIEDIGEKIRIYPTAK
                     EKTEENVEE"
     gene            complement(16182..16463)
                     /locus_tag="PKK75_00575"
     CDS             complement(16182..16463)
                     /locus_tag="PKK75_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HNZ82116.1"
                     /translation="MSKFNNFRSKLADELEIPNNALSDNFELTMHGNKKVIIENHLGL
                     TVYDENMVSINTNEQNITIKGKKFKIEEINSYKVIVKGNIKEIIFSIKE"
     gene            complement(16558..17001)
                     /locus_tag="PKK75_00580"
     CDS             complement(16558..17001)
                     /locus_tag="PKK75_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNZ82117.1"
                     /translation="MPLKEKLMEDLKQSMKSKDKVKKDTVTMVRAAIKQREVDERIEL
                     SDTEIVDIIAKQIKQKKDSIPDFEKGSRQDLVDLTNEEIKILLEYLPPQLSDEDLESI
                     VKEAIEATGAQSKKDMGKLMAFIMPKVKGKADGKHVNEIVAKYIK"
     gene            complement(17014..17202)
                     /gene="rpsU"
                     /locus_tag="PKK75_00585"
     CDS             complement(17014..17202)
                     /gene="rpsU"
                     /locus_tag="PKK75_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNZ82118.1"
                     /translation="MTEVKVRENESIDNALRRFKRQCALTGVMSEVRKREHYEKPSVK
                     RKKKAEAARRKKNKKARG"
     gene            complement(17312..17653)
                     /locus_tag="PKK75_00590"
     CDS             complement(17312..17653)
                     /locus_tag="PKK75_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNZ82119.1"
                     /translation="MDCIFCKIVNKEIPSQLVFENEYMAAFRDLAPQAPVHILAVPKV
                     HIASMNEITKENSHIIAEVFEAINKIAESEGIKDSGYRVISNCGKDGCQSVQHLHFHI
                     LGGKKLSESMA"
     gene            complement(17677..18990)
                     /gene="mtaB"
                     /locus_tag="PKK75_00595"
     CDS             complement(17677..18990)
                     /gene="mtaB"
                     /locus_tag="PKK75_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNZ82120.1"
                     /translation="MNKDTNTEYTVSFITLGCKVNQFETEAMTEILEDEGFKVLPPEE
                     ASDIYIINTCAVTKESERKSRQFINKAKRFNPDALVVAVGCSVQLNSDKINKETQADI
                     IIGTKHKGNIGKLIKDKLKDIKQDYEIDDFQRRERFEELKISTVHDKTRANIKIQDGC
                     SQFCSYCIIPYVRGPIRSRNHEDIIKEVKRIAENGYKEIVLNGIHISSYGKDFKEENC
                     LIELIEDINKIKGIERIRLGSIEPNLINEDFMKRYAALDKTCDHFHLSLQSGSDSVLK
                     RMNRKYTAEEYKNNVALIREFMPAAGITTDIIVGFPGETDEEFNETMEFVKAINFSRI
                     HVFKYSMREGTKAADMEPQVAGTIKFNRSRQLIDLGERISYEFMGGFIGRDLPVLIET
                     QNDDNLYEGYTTNYLKVLLKSDINVRNEIIKVHIKGIRNDYLVGE"
     gene            complement(19200..19547)
                     /locus_tag="PKK75_00600"
     CDS             complement(19200..19547)
                     /locus_tag="PKK75_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNZ82121.1"
                     /translation="MYNKNKGFEYEKIAEKYLLDKGFTILETNFTSKFGEIDIIAQKE
                     NRLHFIEVKGRKNAHYGYPGEAVTHTKQKRLKSAAGYYFMLKGKDDYPCQFDVIEIIL
                     ESKELNHIENAFN"
     gene            complement(19547..21178)
                     /locus_tag="PKK75_00605"
     CDS             complement(19547..21178)
                     /locus_tag="PKK75_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNZ82122.1"
                     /translation="MFADNKIWIGKSDKRVYLEPRMANRHGLVAGATGTGKTVTLKVL
                     AESFSDLGVPVFIADIKGDLASIAIAGTDNENMQERITRFGIDNFTYKGFPVQFWDVY
                     GEGGIPVRTTVTEMGPLMLSRILDLNDTQAGVLNIIFRVADERGLLLLDLKDLRAMAQ
                     YVGDNSNDFRMKYGNISAQSIGAILRNMLNLEDQGGDFFFGEPELNINDWIKTSNDGR
                     GYINVLHCAKLFLSPLLYSTFLLWMLTELYENLPEEGDLEKPKMVFFFDEAHLLFEDA
                     PKQLLDKIELVVRLIRSKGVGVYFVTQNPMDVPDEILGQLGNRIQHALRAYTPAERKK
                     VVTAAETFRENPEFDTVEAITELGTGEALISCLDEKGIPSVVERAFILPPQSQFGTID
                     DMLRNQIIVSAPVYKKYYKAVDRESAYEMLISNAERKQEEQLLEIKRLEEEKRLQEEA
                     REEEKRLQLEAKEEEKRLQLEAKEEERRLKEEAKRLKEEEKERERQEKELEKLKKKAS
                     SRLTPVDRVANSAMSAIGREVGRSLIRGVLGTFKR"
     gene            complement(21202..22155)
                     /locus_tag="PKK75_00610"
     CDS             complement(21202..22155)
                     /locus_tag="PKK75_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82123.1"
                     /translation="MSTVYFIAQQTMYFAIPLLIVALGGMFSERSGIVNIALEGIMVI
                     GAFFGIGFIHIFQNSMSGQSLLLLAVLVSALAGAIFSLFHAFAAINLRADQTISGTAL
                     NMLAPAFAVYTARMIQGVQQIQFMDTFYIRSVPILGDIPILGPILFKNAYITTYIGLL
                     IALASWFVINHTRFGLRLRACGEHPQAADSVGINVYRIRYAGVIISGILAGIGGLTFV
                     VPTSTNFNATVAGYGFLAIAVLIFGQWRTKKVFVAAFFFGIMKTFASAYSGIPFLKGL
                     PISNEVYKMIPYIATLIVLTFSSKNSQAPRAEGIPYDKGSR"
     gene            complement(22156..23412)
                     /locus_tag="PKK75_00615"
     CDS             complement(22156..23412)
                     /locus_tag="PKK75_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82124.1"
                     /translation="MRRTRLLQYEEFTSFLSSLMAILAGLLVGLVILLISNAEDAFPA
                     FLTILKGGFTDGTKGLAQVIYFAIPIIMTGLSVGFAFKTGLFNIGASGQFTMGAFVAI
                     YIGIKWTFVPEGIHWLVALLGAMAAGAVWGSGPGILKAFFNVNEVITSIMMNYIGMYL
                     VNMSIVKTVYDSLKNQTKPVLKSAVLPKAGMDKLFNTSNVSIGIFIVILAVIVIYIIL
                     QKTTFGYELKACGSNKEASRYAGINEKKSLILSMVIAGALSGLGGGLLYLSGSGKYIH
                     VLDVLAPEGFSGISVALLGMSHPIGVFLAGLFIAHLEVGGFNIQLYNFVPEIIDMIIA
                     VIIYCGAFALLFKQIIKRLLFKPVEEKAQDVKEPVKSSVISDDDYPVKPDVEEGIQTE
                     MGAGNESPVVDTKTEENDTVQREEDI"
     gene            complement(23399..24934)
                     /locus_tag="PKK75_00620"
     CDS             complement(23399..24934)
                     /locus_tag="PKK75_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82125.1"
                     /translation="MEYVIEMNNITKIFGNFKALDDVTFNVKKGEVHALLGENGAGKS
                     TLMSVLFGLYQAEKGGIKINGNPVKINNPNDASTLKIGMVHQHFKLVHNFTVLESIIL
                     GRETVNMGILKKDDARKKVIALSERYKLKIDPDAYISDITVGMQQRVEILKMLYSDSE
                     ILIFDEPTAVLTPQEIEELMKIMKELVSEGKSIVFISHKLNEIKEAADRCTVLRKGKY
                     IGTVDVETTTKEQMSEMMVGRKINFNIEKEDIETGNAILEVKNLTVKAKNSKKNIVND
                     VSFSAKAGEILCIAGIDGNGQSELVYGITGLIPIKEGQVLIKGKDITKEPVRKKSLDG
                     MSHIPEDRHRHGLVLDFDLKQNSVLQTYFQPRFQKNGFIKFNAVKEYAKKLIKQYDIR
                     SPRDEDSIVRNMSGGNQQKLIIARELDKDPDIVIAVQPTRGLDIGATEYIHKQLIAQR
                     DHGKAVLLVSLELDEVMDVSDRILVIYEGEIVGDLDPKEITVEELGLYMAGSKRSDKY
                     EKN"
     gene            complement(25006..>25620)
                     /locus_tag="PKK75_00625"
     CDS             complement(25006..>25620)
                     /locus_tag="PKK75_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ82126.1"
                     /translation="FMGGMAVPAVVRFGYGFVQGAEYAAHELGLTEPIQLKYTYVGNF
                     DASPENQAKASSWYNEGTEVIFACGGAVGNSVMKAAEAAGTKVIGVDVDQSAESETVI
                     TSSMKNLSKSVYDALTAYYAGNFPGGTSVSLDATVEGVQLPMENSRFEKFTQADYDAI
                     YGKIVAKQIEIMNDAAVVEDAGKPAEEVTAADIPVSKVQVEVIQ"
     assembly_gap    25621..25720
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25721..26277)
                     /gene="deoC"
                     /locus_tag="PKK75_00630"
     CDS             complement(<25721..26277)
                     /gene="deoC"
                     /locus_tag="PKK75_00630"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNZ82127.1"
                     /translation="MDIHELMKYVDHTLLKAYARWEEIKTLCDEAVKYQTASVCIPPN
                     YIKRIHDEYGDKVNICTVVGFPLGYSTTEAKVAECLQALKDGVNEIDMVINISDVKNK
                     DYKKVEDEIRTIKETAGDHILKVIVETCYLTEEEKIEMCHAVTNAGADYIKTSTGFGT
                     GGATMDDVLLFKKHIGQNVKIKASGG"
     gene            complement(26375..30109)
                     /gene="addA"
                     /locus_tag="PKK75_00635"
     CDS             complement(26375..30109)
                     /gene="addA"
                     /locus_tag="PKK75_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HNZ82128.1"
                     /translation="MNWTKEQKRIIDIKNSNILVSAAAGSGKTAVMVERIITLIKNGE
                     DIDRLLVVTFTRAAAAGMKKKIQSSLVKAVQNREGDVRHLRRQLSLLNRSMITTIDSF
                     CMDIVKKNFHLADLDPDFRVGDESELSILLQESIDEALEEEYEKINDDENFKKLVEGF
                     TGNRGDDELSSIIQSAYKFILSFHDPFSWLEESVRKLSITGEELKNSKWLDEIKSYIE
                     LLLNGAKGYINTAVEICKEPGGPALYLDTLKKDTDIVDNLIALIDRDMKEFMTALKDF
                     NAPRAASFKERDYPDVDIEKQIEVAGSKNNSSMRAQYKAVIASIKEVLPYDLDYDKYA
                     WEINEMYGSLSSLSELIIKTDKNYREKKKEKSIADFNDLEHFALKILRNKKETGEFVP
                     SEAADYYRKKYSYIFIDEYQDSNSLQEAIIEQIRRENNLFMVGDVKQSIYRFRLADPS
                     IFNYKYTAYTRDSSKLSDETINRTIDLNKNFRSRDEILKAVNFVFKNIMSKELGEVEY
                     DEKAALNTGTEFCFNTPVELNIIQKDVSLKGYEKNGDEEDGEDFKEAKSQIELEIESM
                     KTAELEALFAAKKIKELIKEEITIPGNSGTRKIEYKDIVILLRSVSNWAGIFEERFNK
                     EDIPFYYDGGKGYYEALEVKIVVNLLRLIDNIRQDIPLLSLMRSPIGNFTTEELLKIR
                     LGNPKEKYFIDAAGKFIQGEKDGDHSELLSKLKDFIEKIYSWSYKSRYMNLNELIWDI
                     LMETNYYNFAGALPNGKMRQANLRMLADLAYKFEKTSMRGLFKFLRYIEKVTKGADDR
                     GQAKTLGENDNVVRLMSIHKSKGLEFPVVILCGLNKQFNLQDTRNKILMHKDYGMAPK
                     YVNVDERIEKETLARTAIRLKIKKENISEEMRVLYVAMTRAIDRLIMAGAVGNLEQRQ
                     RIWRRGHSEYFVYKGLSYMDWICGCLFEKVNLEKFNEIMKIGQQEGWNLKLLSQYELS
                     MSVNDEFNLKESRIKELNEFKKMENSPHYREIGRRLSYIYPYAASVNVPTKLSVTELK
                     NLEDKEFSKLRYKIPTLVDIFNYNENDDKFLLDKEISGAEIGTLLHFVLEHLDLKGDL
                     KSYGIVNTIKRMEERKLLTEDEAKIAAGTYAEKIEEFFKTAIGKRMVNSDSVYREVPF
                     VLRKKANDVLESLNEEDLILVQGIIDSYFIEDNEAVIVDYKTDRIDERKNVAAQVQKL
                     KEEYEDQVILYKEALEKITGIKVKECYLYLFSIGKEVQIL"
     gene            complement(30096..33530)
                     /locus_tag="PKK75_00640"
     CDS             complement(30096..33530)
                     /locus_tag="PKK75_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNZ82129.1"
                     /translation="MSLRILAGRANTGKSSFIYNEIKSRSEDKKDNLILIVPELMTYQ
                     AESSIIERFGLPGIMNIEILSFKRLERKVLEEVGGLKVQDISVFGKIMLLKQIFEGNK
                     DKLKIFGNSCKQSGFLKEFEKLTAEFKQNLVDSQSLKNAALQVTDEILMRKLSDIELI
                     YSEFIKRSQGKYFDEEDKTNLCISLIKDSAYIRKSKIWIDGFESFNYQRLHLIKALCE
                     NSRSVTASLNIDSSYFIEPDEKDDYEAFKIIYDTYINLKKLDIEIEIISVKDNMYSSP
                     EIKAVEKNMFSLNIEEYENQSVKITVYSSLNPYRETQKTAAEIISLVRDKGYRWRDIK
                     LALGSMDTYETNVKRIFAQYKIPYFLDVKRDILNNPLSKYILSIFDMFIWKFKHDNVF
                     EYLKTGFSPLSIKQVHLLENYALEYGIEGEKWFREFKTSGKDEDNQKPPKQNEMEEYR
                     KLFAGDFETERKEIQNLKRADEITKLIFKYLKKHKVKEKMEKAVKGFNAMHKFEEASE
                     YSQCWNYVMEVFEQLMLVGKDTEISVREYRKMLEAGLGEVEVSIIPPAIDKVEVGEIN
                     RIAVTKPKALFIIGANDSNLDAKNTEKGLLLNEERELLLENHVKLTKGADYDTFKEKH
                     MHYKLFSSPSQSLYISYPMGLETGESLQPSLYIDILKKIFVNMKAGSDLSIGDELESV
                     SNYGGTYDYFVENMRSYIDGYEVDKLWRDVYAWYEKEDKDNFYIINKALKYKNSMDTL
                     EPETVKSIYENNMTMTVSKFETYAQCQFKYFMENVLKPRERVVQKIEFYDLGDIYHSV
                     VEEFINKIKEECKDLSLLDMDRAEEEAVRITNEVLKNQEDKLTAIEANYRNKYMKEKI
                     KRVMKRTCRTLAGQLNSSMFRPAYTELKIDERNEKAETVVPPIEISVDTEKIKEVLKL
                     RGKIDRVDVFENKDKLYVSIIDYKSSPNEVDLEDAKEGIQVQLIMYLKALIDKGEELF
                     GRKTHVGGVFYYFINDPMLTDDNKLKDPEEEIFKKLKLKGYVLKDKEVVKLMDKNIDS
                     TSKVIQAALKKDGNIREDWTKALTEEEFKNVMDIVYEKCADMTRSILEGNINIDPYRK
                     PDGKTPCTYCDYISICQFDSSLGNNYRPIGTKYPASKEKQEILTAAEAEGGYNELD"
     gene            33749..34423
                     /locus_tag="PKK75_00645"
     CDS             33749..34423
                     /locus_tag="PKK75_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HNZ82130.1"
                     /translation="MNNNCYSADIIMHEYNKTFNYNNTEMLKLTIKYPQISLNNKQAE
                     NIINAQIYMEVNNYKKYAKHLFKQAMQAYARSKTTGYPFLGYEAYMEYFITYNENCFF
                     SLYYDRYEFTGGAHGTTVRASDTWELCCGTCLSLYSFFRPDTDYKHLLINEIIRQAEA
                     IQRRQNIYFEDYKSLIIKNFNKDSYYLSPEGITIYYQQYDIAPYSTGIVEFTVPYSVI
                     GWKPNC"
     gene            complement(34488..35417)
                     /locus_tag="PKK75_00650"
     CDS             complement(34488..35417)
                     /locus_tag="PKK75_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNZ82131.1"
                     /translation="MNMKDLDNYILGIKEEADIFSELDNVRLTKLDDVKNILIKRSRN
                     SHKGTYGKAGFVSGSKGMAGACVLNLNGALRCGSGLVKAFIPEEIYYVVESMSLEAIT
                     CTYDADNISFEKLQREIIDYSDVVATGSGCTNINNYSDILNYLLKKCKKPLVIDAEGI
                     NKLDLKNLKNHSQPLVLTPHYGEMVKLLNKDMDYFKTDIIKKSMEFSMDYNVYLLLKG
                     ARTLIACPDGKIYINTTGNPGMATAGSGDVLTGAAASLIGQGIKTENALRAAVYLHGL
                     AGDIGAEILGEYSLTSGDLVKFLPYAIKRMTAN"
     gene            complement(35456..36112)
                     /locus_tag="PKK75_00655"
     CDS             complement(35456..36112)
                     /locus_tag="PKK75_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ82132.1"
                     /translation="MEKNKFHKDTFDKTTEERRQQVLKAAIEEFALKGYNATSINDIS
                     RNAQISIGAMYSYFASKEDLFLSIVNNAYYLMDDILKNISDESSDIFDCVKGMIKASR
                     EFALRHPHLNRIYLDITTQALSHMSIKLSNTIEIITPQILWEFIEKAKKEGKIKNDIN
                     EKIAAYCMDNIFTMYQFSFSSDYYKERLIIYVGDDNLNDIDKLEEGIYKFIEGSLLRR
                     "
     gene            complement(36123..37721)
                     /locus_tag="PKK75_00660"
     CDS             complement(36123..37721)
                     /locus_tag="PKK75_00660"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015843490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNZ82133.1"
                     /translation="MGVYVITYDIGTSGIKTCIFEISDTIELIASAVENYKLYIDNNG
                     GAEQDPDELYNAMCSTTQKVLSYGITKEEIQGISFCSQMQSLVLVDTDGRALRRTMSY
                     MDQRAYLEMKEGIGKGIRISGLNIFKLLKSIRITGAVAASAKDPVWKYKWVKKNEPDI
                     FKRVYKWLDVKEYLILQCTDKFVMTKDSAFGTLLYDVRDGKNCFSREMCKMLGVNYEH
                     MPEILKSSDMVGGLTKKAAEKLGLQEGTPVYGGGGDASLIGIGAGAAGIGNTYIYSGT
                     SAWVSTVVKKRAIDLTSMMASIVGADDDSYNYFAELETSGKCLEWVKDHLALDEINIY
                     LEKVSVSDSEESKFVSLYDFMMEEIKDVPAGSNGVIFTPWLHGNRCPFEDSNARGMFY
                     NISLETGKRDMIHAVIEGIAYHLRWMLEEQKKKVKTSDTIRFAGGGALAPLTCQTLSD
                     VLGCKIETVDNTQNAGALGAAVIAALGMGLLDHIEEANNLIKVKDVYYPNPLNKEVHD
                     RNFNVFKLLYKNNIKTFRMINGMD"
     gene            complement(37728..38903)
                     /locus_tag="PKK75_00665"
     CDS             complement(37728..38903)
                     /locus_tag="PKK75_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNZ82134.1"
                     /translation="MNEREAREKVVSAGKRLVSTGLIARTWGNVSCRISDSHFAATPS
                     GRGYLTLTPEETVVVSIENLSYEGSIKPTSEKRIHAEIYKHNKDINFVIHTHQKYASV
                     LSAMGLDSINTDFSVPGNIACASYALPGTKKLSSYIRAALNRSYGNAIIMKNHGAICF
                     GQDEEEAFQTAKVLEDACKDYIAKQYYKAEKDPNLDSDKKKELEKIKQSLLTTNPPAV
                     CNYSRRTDDGFTMKAEGKEYIYVKHGETGSFDHEDARIHNEIYLKHKNINYIIHTYAP
                     DILNVSSAGITLYPMLDDFAQIVGTKVETVINNPVNIAGALKNSSAVLIKNKGALCCA
                     VSKDDASAVEMILEKNCIIQICKALFKDAKPLSRFDSMLMRFIYLKKYSKKMNEKGM"
     gene            complement(38923..40398)
                     /locus_tag="PKK75_00670"
     CDS             complement(38923..40398)
                     /locus_tag="PKK75_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ82135.1"
                     /translation="MDNSFSISTYPDAGKINKQLDELISKPIYSIKPEALKKYEDEYY
                     DRKCLKSKDMINKAKDLIPGGVQHNLAFNHPFPLVFTKAEGAYLYDIDSNKYYDFLQA
                     GGPTVLGSNPVEVRTKVIELLNECGPSTGLFHEYEYKIAKKISDSMKNVEMFRMLGSG
                     TEACMAAIRIARLATGSKNIVKMGGAYHGWSDQLAYGIRIPGSKWTQASGVPRYVFKH
                     IQEFFPNDLNDLERTLKMNKLHGSTAAVIIEPLGPESGTTPLDKDFNKGVESLCRRYG
                     ALLIFDEVVTGFRVGMAGAQGYFGVSPDLTIFGKVIAGGYPGAGGIGGKKEYMKYLSA
                     GIAGGTKQKKALVGGTLAANPLSCTAGYYTLCEIERTNAVQKSGLMGDRLTAGLEKLI
                     EKYKLPFVAFNQGSICHLETVGTTHFFIDWKKPWTISDVIKKTSLRKKEMEHMGAAYM
                     AEGIITLAGSRLYTSAAYDEDMIDDVFNRFERVFKNIGIIE"
     gene            complement(40435..40911)
                     /locus_tag="PKK75_00675"
     CDS             complement(40435..40911)
                     /locus_tag="PKK75_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNZ82136.1"
                     /translation="MNGLPEEDHTNNIKDIKNIKKETIRSLKFTLFSLSAGIIEIVSF
                     TLLNELTGWPYWACYLTALVLSVLWNFTLNRRYTFRSSANVSSAMFKTFLFYLVFTPV
                     STFLGDYLADTLMWNEYLVTILIMVSNFILEFLYQRFYVFRKSIDTNELAKKKLIE"
     gene            complement(41037..41729)
                     /locus_tag="PKK75_00680"
     CDS             complement(41037..41729)
                     /locus_tag="PKK75_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNZ82137.1"
                     /translation="MKKEIRNSAKKIIITCILIFACLSMYLIETYVKPAYIFKSVYKI
                     LLFAGLPLCYSLIDKNFKFKEYFIINDKRQIFIAAVLGISVYFVILIGYFIIKGFIDL
                     DNITIQLNNNLNVNKENFIFVALYISFVNSMLEEMFFRGFGFLTLGKHSSYKYSILTS
                     ALAFSIYHVSILANWFHIVIYIVFILGLFFTGLFFNWMNLKNNNLYNSWIVHVSANLS
                     INTIGFIMYGMI"
     regulatory      41947..42045
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            42143..43042
                     /gene="metF"
                     /locus_tag="PKK75_00685"
     CDS             42143..43042
                     /gene="metF"
                     /locus_tag="PKK75_00685"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNZ82138.1"
                     /translation="MNIKEKFKEKRLLISFEIFPPKPDYPIDTIYKTIDELHSLAPDF
                     MSVTYGAGGSTKDRTVEISSYIQNKYNLTSNAHVTCISSSRNEIENISNSLKENNIKN
                     VMALRGDYPKDYIPGSLPSDRYKNSVDLIEYFKKNTDFCIGAACYPEKHPDARDLNAD
                     IDFLKRKQDAGADYLVTQLFFDNGIFYDFYDKTQKKGISLPISAGIMPILNKRQINTI
                     VRLTGNSIPKRFMRLLEKYENNPEALNEAGMSYAISQIIDLLSWGVAGIHVYTMNKSS
                     TAGKILESISAIRGVLTNERKIS"
     gene            43023..43715
                     /locus_tag="PKK75_00690"
     CDS             43023..43715
                     /locus_tag="PKK75_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNZ82139.1"
                     /translation="MSVKYLDNDLIKIDKTEALRYLRYKNEDVDENTGRLLDESAAEL
                     KKICRLKYVFRIFNLEKEDNNISFENHIKIKSDDLKGLFKHCDKSAVMAATLGFETEK
                     RIRYYSMADLSKALVFDALAAACIESLCDACEAEIKEIAEKEGCTITFRYSPGYGDVP
                     ISHQREILSALDAQKLIGLTVSDSSILIPRKSVTAFIGFDKSRNFYKNSCLNCSFFQS
                     CDFSKEGDNCVK"
     gene            43699..46065
                     /locus_tag="PKK75_00695"
     CDS             43699..46065
                     /locus_tag="PKK75_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNZ82140.1"
                     /translation="MTVLNKLKEKFLYFDGAMGTMLQNSGLKVGELPEELSITKPEII
                     YNIHKDYLNAGSNIVTTNTFGANRLKLKGSPYRVEEIIAASVNTAKKSIAGMEEKYVA
                     LDIGPIGTLLSPLGTLGFDEAYEIFKEQVIIGTKEGADLILIETMTDLYELKAAILAA
                     RENSDLPVFATMSFQDNSRTFTGTDVKTFVFTAEGLGVDVLGVNCSLGPKQLQPIVDE
                     LVEFSSIPIMVQANAGLPKYINNASYYDISPLEYSREINIMAKKGVAIFGGCCGTTPE
                     YIKSIINSLNKMKPVEISKKYFTTATSSSKTVFLGQEVKIIGERINPTGKKRLKEALK
                     ENNLDYIIREAIIQQETGSDILDVNVGFPGVDEKQMMKKVISEIQSVVSLPLQIDSAN
                     YDVIEAAARMYNGKPLINSVNGKKESMEKLFPIVKKYGASIVALTLDEEGIPSTAEKR
                     LEIASNIVEKAKEYGIAEESILIDPLVLTASAQQTAVMETLKAIPLIKNKYKVKIILG
                     TSNVSFGLPCRNLLNSTYLAIALGYGLDAPITDSTNQVLMDVVRAYKVLAAQDKDSRD
                     YIEAYGNISPESNVITDDYDLKEIIVKGLKDEARKVSLELLKKHKPLEIVNEFMIPAL
                     DVVGEKYQKQEIFLPQLIRSAETSKEVFDTIKEKSTKTGEIEGKKILLATVKGDIHDI
                     GKNIVKLILENYGYKIFDLGKDVDAERIVETVGKEKIELVGLSALMTTTAKNMEDTIK
                     LLKSKFPGIKIMVGGAVLTADYASEIGADYYSKDARGTVDIAKNAFNS"
     gene            complement(46165..46398)
                     /locus_tag="PKK75_00700"
     CDS             complement(46165..46398)
                     /locus_tag="PKK75_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNZ82141.1"
                     /translation="MEYTVLFFTHSGAIKFDRKCKRKNIHCELMPVPRILSSNCSVCA
                     KIECNDIEGLMDDEIEKIFLTQNNENKLVYESE"
     gene            complement(46400..46606)
                     /locus_tag="PKK75_00705"
     CDS             complement(46400..46606)
                     /locus_tag="PKK75_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNZ82142.1"
                     /translation="MKKIDTCGMSCPQPVLMTKKALGENPEGLDIVVDNSTARGNVER
                     FLKSAGYTATITESGDTFLIEARK"
     gene            complement(46616..47707)
                     /gene="yedE"
                     /locus_tag="PKK75_00710"
     CDS             complement(46616..47707)
                     /gene="yedE"
                     /locus_tag="PKK75_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HNZ82143.1"
                     /translation="MKDKRGIIIAGGLVGIIAVLLVKLGNPANMGFCIACFIRDIAGG
                     IGLHRAEVVQYIRPEIIGLVLGAFSMALINKEFQTKGGSSPFTRFMLGIVVMIGALIF
                     LGCPLRMVLRLAGGDLNALLGLAGFVAGIFVGVVFLNKGFNLKRSYNITKIEGYIFPA
                     VNVGLLVLLVVAPAFLFFSAKGPGSMHAPLAVSLAAGLIVGALAQKTRMCTVGGTRDL
                     ILFKDMYLLSGFIAILVAAFAGNMVFGFFKLGFAEQPIAHTDGLWDFLGMAVVGWGSV
                     LLGGCPLRQLILSGEGNTDSVITVMGMLVGAAFAHNFGLASSAAGVTPNGKVAGLICF
                     AFLGLVSYLNSEDLVKKTASEGLSKADAK"
     gene            47999..49153
                     /locus_tag="PKK75_00715"
     CDS             47999..49153
                     /locus_tag="PKK75_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ82144.1"
                     /translation="MRYVYLDNGATSFPKAPGVAESISDYILNIGTNVNRGAYSSSFK
                     AENTVYETRELLCELFHFDKPENVVFTKNITESLNVLIKSLLKENDHVVVSSMEHNAV
                     MRPLNSLKGVEYTRVLCDETGNLPVEDVEKAIKKNTKAVIMTHASNVCGTVLDLEKVG
                     QICGKHNLFFIIDSAQTAGFLDIDMDGLNADAIGFTGHKSLLGPQGIGGFIVKDRMNN
                     EMTTFIEGGTGSLSDTEIQPGYMPDKFESGTLNIPGVYGLNASLKYLLKKGVKNIREK
                     EISLLSRFLDGVLNIENIKLVGKTTTLDRTGILSIDFINNDNGLVSHELSKEYGIMTR
                     SGMHCAPSAHKTLGTFPKGTVRFSVSHFNTLEEIDYAIDCINKIAKIDNC"
     gene            49197..50096
                     /locus_tag="PKK75_00720"
     CDS             49197..50096
                     /locus_tag="PKK75_00720"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HNZ82145.1"
                     /translation="MEFNQLESFVRVVKHKSFSLAAKELYTTQPTISNNIKNLEKELN
                     TTLLDRTSKTINLTDSGKLFYKFAVEMINTRDKAKFTISEHDNKVEGVIEICASSIPE
                     EYVLPYIIKDFLKIYPKVSFSVTHKNSKNVIDDILEGRLNYGIVGAKYYSELLEYIDF
                     YEDELILCLPAGSKCLWSENEHMDIKQLFSEKFIFRKPGSGTRRLIEQRFSEKGISLD
                     DLNIISYIDSNEMIKKMIELGLGISFISKIAVKNEIDLKLVKTMQIKDLDLKRSFYFV
                     RNKNRTLSPLVEAFKNFLISWKY"
     gene            50113..51153
                     /gene="selD"
                     /locus_tag="PKK75_00725"
     CDS             50113..51153
                     /gene="selD"
                     /locus_tag="PKK75_00725"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:50161..50163,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNZ82146.1"
                     /translation="MPSNDIKLTQMTKSSGUAAKIGPDVLAQVLCHLNYDSDENLLVG
                     MDTSDDASVYRINDNLALVQTLDFFTPVVDDPYVYGQIAAANSLSDVYAMGGEPKLAL
                     NITCFPADLPKDIVKSILKGGHDKVKEAGAMVIGGHTVEDTEPKFGLSVTGFIHPKDV
                     VTNSNAKPGDVLILTKPLGLGILNTGIKAGIVSEEGYKTAVRTMTELNKFSKDAMMKV
                     GVNSCTDITGFGLLGHAFEMAEGSDVTIKFNAMSIPIIEEALSLARMGIIPAGAYRNL
                     GYIKNQVKISDKVPQEVTDCMTDPQTSGGLLISVEKNKAEMLLNELKNSITPYAQIGE
                     VLEKQEFRIIVE"
     gene            complement(51226..52140)
                     /locus_tag="PKK75_00730"
     CDS             complement(51226..52140)
                     /locus_tag="PKK75_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82147.1"
                     /translation="MKKIVAFILVSILLIISSCSRYEDNNENLELSYNGNENTGSIDE
                     NNSIENTDNDENNQDLNKTGENNSDGINPDESNSDVNNDREESSGNDSGEAEAEEAIR
                     EGNVKIVYPNGDIYEGQMKNGMMDGYGLYTYPNGDVYEGEFKENVMQGHGKITSPNMY
                     IYEGQIKNGMMDGFGIYKYSNGDVYEGEFKENVQHGNGKFTVPNRYVYEGEFKDDFIN
                     GHGVTVYANGDKYEGEYVNGMKEGLGTYTFANGNKYVGEFKNGKKEGSGVLYYIDGSF
                     EEGKWEDDSFVYNNQQDNDINQIIEIID"
     gene            complement(52313..53518)
                     /locus_tag="PKK75_00735"
     CDS             complement(52313..53518)
                     /locus_tag="PKK75_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ82148.1"
                     /translation="MNKKFGYGYVVALMCFIIAFFYIGYANNTSSLYVVPVTGYYGFS
                     RAEFSLIFSIISMLSIFANLVFVKLYKRLGIKKLVIMGTFFCASAYFVYFRSTKLIQF
                     YTGAILFGIGHAYTNMIVFSLLINSWFIQNRGTILGLISAGSGFGGSVMSPVVGYIIS
                     VYGFKQSYMLTSIILFILIIPIALTIKENPVEHIETNHTEVSNFKKKTTRELLNEHRV
                     ILGLTVIFLMGFLMAPWLNVLPSHLMDRGFDEIFASKILGGVMFIMALSKILVGAVYD
                     RIGIKAAIFICLVSFVICAVLLLIVNSEIIAWIFAALFGISLSALSVLLPLFTSAISG
                     DEYLSDIIGIASALVGAGIALGTPVINFTYDLTGSNNMILITFTILGIINMFLTLLAL
                     RKTETTSKG"
     gene            complement(53578..54279)
                     /locus_tag="PKK75_00740"
     CDS             complement(53578..54279)
                     /locus_tag="PKK75_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNZ82149.1"
                     /translation="MKYTIDKSVFELNPDIKFGILIGSNIKNSSTRPEDEARLRRAED
                     KMRQEVKADEIRNLNNVRLYREVVMKSGINPNKYLPSVEAMFKRIVKGGQLPVINALV
                     DLCNAVSIEQGISLGGHDLIDIHEDLEVRYSRKGDIFLPFGSETYEDVEENELIFTSG
                     NIVQTYKWLWRQSELGKLTLDSSDVFFQLVGFDYEEGSSLFEAMRDIEQLVINRFQGS
                     CRKYLVDINNQSIEF"
     gene            complement(54314..55186)
                     /locus_tag="PKK75_00745"
     CDS             complement(54314..55186)
                     /locus_tag="PKK75_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNZ82150.1"
                     /translation="MKRSDGYLLKDLKPFVKIIPYVMEKRSDSQDFSKQVIPAENIDK
                     YILEKKQAGYNFSYMHVFIAAYVRVIAERPMLNRFVMSSQIYQRNKIYLSMAVKRSFR
                     DDGEETTVKFEFTGKENIFEIAEIIDKDIDRAVSSSEDNNTDKLAERVMSMPGFVKKI
                     LVRIIKAADMLNLLPKNVIDASPFHTTMFFTYLKSIDIQYIYHHLYDFGTTGLFAALG
                     KTVKLPVVENDQVVIKKCCEIGYTMDERICDGVYYARSFKLFEKYINNPWLLESEHIE
                     KIQKEKNIKNIVVQ"
     gene            complement(55395..56285)
                     /locus_tag="PKK75_00750"
     CDS             complement(55395..56285)
                     /locus_tag="PKK75_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ82151.1"
                     /translation="MEIAKVKGNTYCIDTGTIYIPFYKLNKQDIILMDSGYHKELDGL
                     EEVLEAYNLRIKTILCSHAHPDHIGNNAYFKKKYDCKIAMTKDEAFICTSFASLKLFY
                     DQPMSLIKSYYNYSICETDIIIDEKQDNIILDDVVFKLFHVFGHSSDQICITTPDDVC
                     YLADALITHEAMRGIKIPYAYVLKDDLESKKALHQLDSNTYIITHKGILYDIEALIED
                     NIKYYGGMAESVYEEITKPMTFEDILKRIIRKFNMEVMSVYKYKMAERMLKSYVEYLC
                     DIKKVKTIIDNCFVKYVRTK"
     gene            complement(56414..>57096)
                     /locus_tag="PKK75_00755"
     CDS             complement(56414..>57096)
                     /locus_tag="PKK75_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ82152.1"
                     /translation="NTKALLINTPNNPSGVVYSKEEIKELSDYLKKMQEETGQTIYLI
                     SDEPYRELVFNDEPVPFIPNYYDNTIICYSWSKSLSMPGERIGYILVNDKCEDNETLI
                     AAVGGAARALGYVCAPTLFQLVIEKCIDVEPDLEVYKKNRDILYNGLTEMGYNVAKPD
                     GAFYLFIEAPNGNSQEFSEKAKAHDVLVVPGAGFGTSTHLRLSYCVDTEKCEKALPVF
                     EKLIKEYK"
BASE COUNT        16244 a        11786 c         9002 g        19864 t
ORIGIN      
        1 attatccaat attcatttcc tgatattttc acatttttta atccataaat caaaggaagc
       61 atcacttggc attttccagc cgcttcttgg ggacaaggat acagacccta ccttgactcc
      121 atcgggcata caagaccgtt tgaactgttg ggtaaaaaac cttttataaa atttctttaa
      181 tgttgaaaga atagtctcag ggtcgtaatc gtccttgaag gcgatatttg ccaaataata
      241 tattttttcc ggtccgtagc cgtttcctag catgttgtac aggaaaaaat cgttaagttc
      301 gtaagagccc actgaatttt cagtaagctg gcggatattt ccttccttat caggaggtag
      361 cagttcaggg cttacaggag tatcgcatat atcaagaagt gtcttctgca ttttacctgt
      421 tgttttatta tccgcatacc actttacaag atattttaca agggtttttg gaactcctga
      481 atttacgctg tacatggaca tgtggtctcc gttgtaggtg caccatccca aggcaagttc
      541 ggataaatca ccggttccta tcacaattcc cccttcctga ttggcaatgt ccatgagaat
      601 ttgagtcctt tctctggctt gggcattttc ataggtaaca tcatgatttt caatatcatg
      661 gctaatatcc ttaaaatgct ggatgcaggc atcctcaatg gatatttccc tgaggcttgt
      721 gcctagttca gcagttaaaa cgtgtgcatt gccgctggtt ctgtctgatg ttccaaatcc
      781 gggcattatt acagatataa tatttgtaag gggcagcctt aatttcttaa atgcttcaat
      841 tgtaacgatc aaggccaggg tactgtccaa accgcctgat atgccaataa cggctttttt
      901 cgaattaatc tttttcatac gctgagaaag acctgcagcc tgaatattta taatattgcc
      961 tgctcgtctg tctctgtttt caggatttga aggcacaaag ggtcttgggt caataaatct
     1021 ttcaagcgta agattctcgc tccaccctaa attgaaattg acaaatttgt agtctttctt
     1081 tatgtgagaa ttattattgc ggcttattct ttcattgctt attttctcta tatcaatatc
     1141 agaatatata aatccgccgc ataaattatc ttcctttaat attcttccgt tttctgcaat
     1201 caggcaatgc cctgaatata ccgaatcagt tgtgctttct tcttcacctg ccgaagaata
     1261 aacataagaa gttatgcatc ttgaggattg gatcctgaca agctctctcc tgtaatcagc
     1321 cttgcctgca gtttcagtgc tcgctgccag gttaagtatt atatttgcac cctgcagcgt
     1381 atggtaagag cttggaggta tatttgcccc taactcctcc cccatatcca cacctatgca
     1441 aagtgaggat atattgtcct taaaaaggag gttttcatta aagagagctt cctggtcgca
     1501 catgacaatc gtttcccaca tcctgctttc agcaggagca aaccatctca tttcataaaa
     1561 ttcacagtaa tttggtatat atgtttttgg aataatgccc agaatttttc cttcttttaa
     1621 taatacggca cagttataca gccgggcgtc agctttcaca ggcattccca cagctataag
     1681 catgtttaaa taccttgttt cctcaattaa gaggtttagg gcttcttcac attcatttat
     1741 taaagtattt tgataaaaca aatccccgca gctgtatcct gatatcgata gttcgggaaa
     1801 taccagtact ctgacacctt tttttgcagc ctgcagacag atttttataa tttctttaac
     1861 attgtaatca ggatctgcca cttttaatga aggtactgcc gaagcaactc ttatgtactc
     1921 aaaattctta tccataacaa cctctaaatt attttttcta aagtcatgtt tcttttaaag
     1981 tttattattt gtatatttgt tttctttaat acttcctcta ttaattccat tgaatgcact
     2041 tccgtgttat aatgacctcc atctattatg cataaattgt tttttaaagc ttcctgagcc
     2101 tggtggtatt taatgtctcc tgttatgtaa acatcagcgc cttgccttat tgcgtcctgg
     2161 atgaattcgc ttccgcttcc cccgcagaat gcaacctttt tgactgtttt ttcatcattg
     2221 cagtatagtt taatatagtc ggctttaagg gcttctttaa caaatttgga atactctctt
     2281 atgtttatgg gagcaacctc ccctattccg ccgtatccgc tgttatctat attaaccgga
     2341 tgaagaacct gataatctga aatattgagc ttttccgcca gattataagt aactccctta
     2401 tcagccatgt catagcttgt atggatgctg taaagattta tattttcttt gatcagcata
     2461 taaattattt gaccttcatg tgtatccctg tcaatttttt ttatggaact gaagaaaaac
     2521 ggatgatggg ttattattaa atccgctttt tctttaactg catgttttgc tgcttccata
     2581 tccaggtcaa aggttaacat aattttattt atgttaactt ccatcgatcc aatttggagt
     2641 ccgctgttgt cccatgactc atggtcttta agttttactt catcattcaa aaaattaatt
     2701 attgaagata cattcatttt attaattcct ttattttttc gtttttatct cttaagaact
     2761 ctatttttac attatattcc acattgcctg ctttttcaag acttctgatg atttttttgt
     2821 ttgtattaag tactttattg acatattcaa tcataacagg gtccttttta tctattaagt
     2881 tcttgctgat ttcataatat aggctgtcct tatatttagc agtgcctgat tttgctttga
     2941 ttacgaaata aaaatgatag ccttccctta ctattttttc atcaataatt tcaaagccat
     3001 tttctgcaag ataccgcctt aaatcatctg ctgccgtcat gggctgcaaa ataagctgat
     3061 cagcattcaa tgcaatgtct tttgaagttt ccagtataaa agaaataagt tctccgccca
     3121 ttcctgcaat tataattgca tcggcttccc cttcctttaa aaccgtgagg ccgtttccaa
     3181 gtctgaaatc gcatctatca ttcattttaa cagaggacaa atgctctatg gctctcttta
     3241 atggacctcc gttcaaatct gctgcaatta ttttatcgca ttttctttct cttaatagca
     3301 tctctgcaac atatccgtgg tctgtgccga tgtctgcagc aactctgcag ttatccacca
     3361 tggatacaat acattctaat cgattcataa taacccctta aatgaataaa tggaatgtaa
     3421 acattccatc tatattttaa tattcgcagt tacttggaaa agtcataaaa atacaaagcg
     3481 aacatttgca ggatacgatt caattcttag cgaaggtcgg ctgaaaattc gttaagcagc
     3541 ttgcactgtt tgagccttgg cgagtttgca agctgtagga tttttagtcg gaatgagcta
     3601 atagaattat tcgtatccga aaccgtgagt gtggattttt atgacttttc caccttaaac
     3661 agtatctttc tattccaaat aatcctttaa tttcttgctt ctgcttgggt gtcttaattt
     3721 tctcagagcc tttgcttcta tttgacgaat tctttctctt gttacatcaa actccttgcc
     3781 cacctcttcc agagttcttg cccttccgtc atccaaaccg aaccgaagcc tcagtacctt
     3841 ctgctctctg tctgttaatg tgtggagcac atttgacagc tgctccctta aaagagtgaa
     3901 agttgcaacg tctgaaggtg cctgtacttc atcatcagga ataaaatctc caaggtggct
     3961 gtcttcctct tcgcctatgg gtgtttcaag ggataccggt tcttgagcga tttttagtat
     4021 ttcccttacc ttgtctggct ccatttccat ttccagagct atttcttcgg gtgtggggtc
     4081 ccttcccaat tcctgcaaaa gctgtctttt gattctgatc agtttattta ttgtttccac
     4141 catgtgcacc ggtattctta tagttcttgc ctggtcagct attgcccttg ttattgcctg
     4201 acgtatccac caggttgcat atgtagagaa tttaaaaccc tttgtataat caaatttttc
     4261 caccgctttt ataaggccga gattgccttc ctgaattaaa tcaagaaaaa gcatgcctct
     4321 tcccacgtat cttttagcaa tgcttaccac caatcttaaa tttgcttcag caagctgttt
     4381 ctttgcttct tcgtctccgt cctccatctt tttggcaagt tctacttcct ctaaagcagt
     4441 taataaagga acctttccaa tttctttaag atacatcctg acagggtcgt caatgttcac
     4501 gcctttaagt atatcttcgt cagactggct tacaaagaaa tccgcatcgt cttttacatc
     4561 agcctcatct tcttcatcaa ccttctcagg tatcatatca tcctggaaac ccagtatttc
     4621 aagatcgttg tcttctgcct gtttataaaa atcatctatg aattccggat tgatatcaag
     4681 tctttcaaat gaccttaaaa cctctttata tgttataaaa ccgtttctct tgcctttttc
     4741 aattaattta ttctttatgg agtctatctt ctgtaacatt tcagcgtttt taactaattc
     4801 ctcttctgaa attacagata tatttttatt atcattcatt gtcagttgac ctcctcctta
     4861 gttgatttaa tctttttagc gaggcaaaaa atttcattca ttatttcctt actgtttaat
     4921 gaattgtcag attgttttaa actgtttgtc aatatgtttc tcttttcttc ataatatttt
     4981 aatttaagtc ttttcataca atccttgaat aaagcctcta aattatcata atcaacattg
     5041 tcaatctgca tatttacatc catgctgcca tttgtaattt tatataagct ttcactatca
     5101 ataccagtat cgttaattct gcattcatac agctctttaa ataatttata gaactttaag
     5161 tccttgaagg tatcttcctt tactatctcg ctcagcttta atgcaaaatc attgtttaaa
     5221 agtatcagtt ttataagctc ttcttcgtgg catgttgtaa tttttttaac ctgcggctta
     5281 atgacaggtt ttgcagcctt aaccaaagtc ttttgtttag tacttctctt attgtattca
     5341 ttaattatag actcttttga aacacctgct tttctggata atttttcaaa aatcaattct
     5401 ctttctattt cgctgtttac atttactata ttgtcaaaaa aattgtttat atattcaact
     5461 ttgttagaat tttcaaggat ttctttgaaa tgaaattcta aaaagctata ataatctaat
     5521 gaattttcga ttaatttgtc gaaacctgac ttcccaaatt tattcaaata ttcatccggg
     5581 tccttcgcat ctttcattat aagaaccttt gccttcaaat tgttcttttt aagcagatta
     5641 agggctttca gagaagcctt ctggccggct tcatccgaat caaatgcaat gtaaaaattt
     5701 ttggagtatc tttttaaaag ttttacctgg ttttctgtaa aagaagtacc taaggctgca
     5761 acagccgtgg aataaccatg aacgtgcatt tttattacat ccatatatcc ttcaaccaga
     5821 ataaagctgt tatcttttgc attttcttta gctatatgta ttccgtataa attatatccc
     5881 ttgttaaaaa ccaagctttc gggcgaattc aagtatttgg gcagcgaatc atccaatact
     5941 cttcccccga accctattat tcttttttta acatcaaata tagggaaaat tactctgttt
     6001 ctaaaccggt cataatatgt atcgtttttt tcgtttctta ttattaatcc ggcttttaaa
     6061 atgtcttctt ccttataacc ctcatttttt agatgattta acaaattatc ccattcaccc
     6121 atagcatatc caattccgaa caattttatt acatttgatt ttatgttcct ggaccgcaag
     6181 tattcgatta catgaggata atttcttaaa tttttataaa aataagcagc agcatccctg
     6241 ttaatcctgt aaagcttatc tgctgtttct ttattgttgt ttattcttgg cgagttgatt
     6301 tcttccagaa caatacctgc tctgtctgct aaatattcgg ctgcttcaac aaaagtaaaa
     6361 ttcctgtact ttgtcagaaa gcctatgctg tctccactct caccgcagcc gaagcaatga
     6421 aatatctgct tgtccggaga aactataaat gaaggcgttt tttctttatg aaaaggacag
     6481 ttggtgctgt aattagcgcc tgcttttttt aaaggtaaat attcttctat tatatctaca
     6541 atattatttt gatccaagat tctctgtata acgtcagaat ccaacctata gggcataata
     6601 tcaccattat cctaacattg aaaataaagt ataacacatg aaaataagct tgtcaataat
     6661 ttttaatgac aagctgcaaa tttttatgtt cagctttttc cggctgtttt gcggtctttt
     6721 tcaagcttac agccagggtt ttggtatgta caagtcatca aaaaccttca ttgcatacct
     6781 gtcggtcata ccggctatat agtcctttat aatctcttcc ctgctgaagc tgttgttgtt
     6841 ataaatgtta agataaaact caggcagggc atcaaaattc ttagtatagt attcatatat
     6901 tttttcaaca atgaacatta ttttctcatc ttcgcttttt gctatcttgt tataatatac
     6961 attttcaaac aaaaaatcgc gaagttcttt agtcagttct gcaacttcat cactcataac
     7021 aatattattt ttgtctgcag agttatgaat tatgtccatt atcattgtgt ttattcttct
     7081 gccgtggctt gaacccagtt taataataaa cttgtccggt attttagtta tgatattagc
     7141 tctcaccgca tcatcgatat catggtttat atatgctatc ctgtctgaaa tgcttacgat
     7201 ttttccctca agggtagcag gctcacatcc cgaaggatga tttaatatac catccctaac
     7261 ttcaaaagtc aggttaagcc cgggcttgtc atttcttatt tccagaaaat caacaactct
     7321 taagctttgc tcattgtgct taaaaccatt gcacagcttt ttaagtattc tttctccggt
     7381 gtggccaaaa ggagtatggc ctaaatcatg acctaatgcg attgcttcgg ttaaatcttc
     7441 attcaagttc aagcttctag caattgttct tgatatctga gacacctcaa gggtatgtgt
     7501 aagtcttgtc ctgtagtgat ctccttcagg tgacaaaaaa acctgggttt tatgtttaag
     7561 cctcctgaat gattttgaat gcagtatcct gtccctgtcc ctttggaagt cagttcttat
     7621 ttcacatttg ggctcatctg tccttcttcc ctttgaatgt aatgccttgg ctgcatattc
     7681 agacagatgg tctatttcaa attgctcata tttttctctt atattcaaac aatcacctcc
     7741 gaagaaatag agttgaaaaa atagggttcg caggggcatt caccaatttt ttagttcgct
     7801 attactcaca aaaattggga atactgtgcg tttgacatac catcaatttc tttctcccta
     7861 tcggtcaaat aaattgggta tgtcataaag ggcatcttag aactttattt tttcgtttat
     7921 gaaggtcacc agatcatcaa tttttattct aacctgctcc attgtatccc tgtctcttac
     7981 agttactgta ttatcttcca aagaatcaaa gtcaaatgta atgcagtaag gtgttcctat
     8041 ttcatcgtgt cttctgtatc tttttcctat gctcccgccg tcgtcataat caatgttaaa
     8101 atgcttgcta agagttttat aaagctcttc cgcctgttca ttcaatttct ttgttaaggg
     8161 aaatactgca gctttaaagg gtgccaaagc aggatgaagc ttcaaaacaa ccctgtccgt
     8221 tccatcttcc aatatttctt cattgtatgc atcaaccagg atggccagca ttacacggtc
     8281 cacacccacc gatggttcaa cacagtaagg tatgtacttt tcatttgtaa caggatctac
     8341 ataatataag tcttctcctg atgtgtttat gtgctgctta aggtcataat ctgttctgtc
     8401 ggcaattccc caaagttctc cccagccaaa gtggaacaaa tactcaatgt ctgacgtacc
     8461 cttgctgtaa tgtgacagtt cagccggttc atgatctctg aaacgaagtt tttccttgct
     8521 gataccaagg cttagaagcc agttcatgca gaattctttc cagtagtaga accattccat
     8581 atcttcaccc ggtttgcaga aaaattccag ttccatctgc tcaaattctc ttgttctaaa
     8641 tgtgaaattg cccggtgtaa tctcatttct gaaggatttt ccaatttgac cgataccaaa
     8701 aggaattctc ttacgcattg accgctggac atttttaaag tttacaaaaa taccctgtgc
     8761 agtttcaggt cttaaataaa gctccgactg ggaatcctca gttactccct gaaatgtttt
     8821 aaacataagg ttgaattttc ttatgggtgt aaaattttgt gctccgcatt caggacattt
     8881 tactttattt tcagaaacat atgcttccat ttcattgttt gtccagccgt cgactatttt
     8941 tacatctcct gcttccttca tataatcctc tataagcttg tcagccctga atcttgcctt
     9001 gcattccctg cagtccatta aggggtctga aaatcctccc acgtggccgg aagcaaccca
     9061 ggtagtggga ttcatgagta ttgcagcatc cagtcccaca tttaaggggc tttcctgtat
     9121 gaatttcttc caccatgctc tttttacatt gtttttaatt tctacgccca aaggaccata
     9181 atcccatgta tttgccagac ccccgtaaat ttcagagccg gggaaaataa tacctctgga
     9241 tttagatagc gatactaatt tttctaaact gtttttttct gtttttgcca taagttacct
     9301 ccatttatat atatgattaa aggatgtgat ttctgttatc ttgcacaagg gtgaatgtat
     9361 tgatccgcaa gaaaatgatg tttcgtccgc gaatttattt gcatacaaaa atctttcatc
     9421 ccatataggg acgaaagatt ttacttccgc ggttccaccc tatttgacaa ggcactgcct
     9481 catcctctta attcaatgac tcaaaagctc ccttcatcca agcatccttg tcagctttca
     9541 ccctgtctga ctctctgcaa attcagcata gactactctt ctttctcaaa gtctctgttt
     9601 taatacaatg taacaaaata atctttttat gtcaatattt tctttttaaa attgtccgtt
     9661 attttggtca aatgaatctt ttatatcatc ctttatgttt tctgctcctg ttcttatgtc
     9721 atctttcatg ttttctgcct cggtttttan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnna tgttttctgc tcctgttctt atgtcatctt tcatgttttc tgcctcggtt
     9901 tttaaatcat ctctcatatt ttttgctcct gattttaaat cctgcactgc atcttttaca
     9961 gcatccattc cttcatctac cttttgtccg atatctatta ctttcccgtc tgtctgagta
    10021 atttccacag agcattttgc aagaagggct actgttattc ctgctgcaga aagaaaaggt
    10081 gcaataaagg caccgaccaa gcctgcagtt atgggtatat tgattataac ttcattgttt
    10141 ttctttattg taagttttgt agcattaccc tttgccaaaa tgtcctttaa ctgggacatt
    10201 atctgctcgc ctctgtttga gcctgacctg ccgccctttt gacggccttc aatcaggatt
    10261 atagcttcca gcacgtcgcc gtttgcagca tccagggcct cttttgcagt agcatagtca
    10321 gtatttgtgc gccttaaaat ttcatcgatt ttttgcaaat tatcattttc catattggta
    10381 tcctttctta taatagcttc agtgaagtaa aattgtccct gcctatataa taaaacagga
    10441 attttctgat taaattcatt atatgcctgg ttacttcaat aaaaccaata ttttcaaatt
    10501 tagattttaa aattttatcc ataattagta tttcattata agtaacattt attccatttc
    10561 caaaatttac gcaatcagag caaaagactc ctccctccgt tattgaaaat ctggcatttt
    10621 catttatcga agatccgcag tggagacaat ggagaaaatc aggcttgtat cctagcattg
    10681 ctgccagttt caattcgtag gcagcagtaa gcttgtcaaa atcctttgta aaattgctca
    10741 gatgagaaag tacggacaca gtcatgtcaa acactctcat atcatattcg ttttcctgtg
    10801 ccacatagct tataagctca agtatataac aagcgttata gtaggcatcc atgttgtttc
    10861 tcaagttgta aaaattatcc acaagccttg cagaagttaa tatatacata tcctttgaag
    10921 gattcagggt gcagcttgaa tatgcaaaaa tctgggatgc atttattaaa tgagagctgg
    10981 atttttttga cccctttgaa ataatctgaa ttttccccct ggtttttgaa agggcatgaa
    11041 gtattttatc attttcctta tatgatattt ccttaagaac aagaatctct tcctctatca
    11101 tttatcatac cctaatattt ttaacctgta attatcatca cgccagtttt ctgaaatctt
    11161 tacaaaaagc tgcaaattta cctggcttcc caaaagtctt tctatgtcct ctctggcgct
    11221 tttgcctata cctttaattt ttcttccatc cttgccgatt ataatgcttt tgtgggactt
    11281 tttctcgcat aggatatttg ctcttatgtc aacaaggttc cggttttttc tttccttcat
    11341 ttcttctatt tcaacggcta ttccgtgggg tacttcatcc tccaggtaca taagtgcctt
    11401 ttccctgata atttcagaaa caacaaaacg ctcaggcctg tcagttataa aatcttccgg
    11461 aaaatacatg ggtccgggct ttaaatattt aactattagt tttaacaatg catctacacc
    11521 cgtgttaaga agtgcagaca tgggaattat atcatcaaag accttatatt tgtcaaattc
    11581 cgcagcaatg cgcagggcag cctcctggtc aaacttatcg attttattta ttataagtat
    11641 tttttttgcc ttggctcctc tgattttttc aattataaat tcgtcacctg gaccgacttt
    11701 gttaaactca tcggttatca ttattaatac atccatttcc tccaaggcgg agtccacctc
    11761 tgtattcata aattcaccca gctgattttt aggcttatga attcccggtg tatccataaa
    11821 tattatctga gcttcatcat ctgtatagac agtcataatt ttatttcttg ttgtttgagg
    11881 cttatccgac attatggaaa tcttttcacc gattaaattg ttcatcaatg ttgatttgcc
    11941 cacattgggc cttccgatta ttgttacaaa tcctgattta aacatagtct tcctttctta
    12001 gtcaaggtca tcgggtccga agctgttagg aagaagctcc tccagggtat aaacactgta
    12061 aaaatccaca ttccttgcac aaataacctt tatgtctctg ccgaattcag acataaactg
    12121 cctgcatacc ccgcagggaa atgaagtttt aatttcgctt ccaactactg caattttgtc
    12181 gatgtccctg taaccttcag aaatgcattt tgaaacagca actctttcag cacagatggt
    12241 gggagtaaat gatgccgact ctatattgca tcctgtaaaa accttgccgc ttcttgtata
    12301 aactgcagcc cccactttgt atttggaata cgggctgtat gatttttccc ttgcctccat
    12361 ggcaattttt ataagtagct tatcttccat ttcattgtcc cctttcttat catttcaaca
    12421 atattagggt acgcccccgt cactgcaatg aataatgtga ccctgccaga aattttattc
    12481 aagatttaaa ttttctaaac tgttgacaac ctgtatccag gtattgccgg gatttagcac
    12541 cagctcttta tcacctgcat aaaatttcag cctgtccttt tcagtagttt tttcccatgt
    12601 tacaggaatg ctttcaccgt ttgtaatata aatgcccttt ccctcaccga tagttcctaa
    12661 atacaatctt ccctcattgt ccaatactga tttggatgtt tctattgcaa taatattttt
    12721 tgcagttatt tgttttttat ccaattcatc gatatgttct tccttatcct tatatcttag
    12781 atataagcct ttttcatcat cgtatatgta atcagttgta ttgtaatcat tgtatactat
    12841 atttactttt cttgctgtag agatttcaaa gttttcagat aattttccat cattttcatt
    12901 gaaggtatag ccttcgaatt ccgcttctgt tctgtatccg tattttttcg cctcatttct
    12961 tatggactcc agggttgtgt acacgttgtg aggtgcatac tttcctgtat cgttgtatct
    13021 ccacatggct cctgaataga gtccgtccac atcagcaaca cctaagcttt ttatcttttg
    13081 gaatgcgtct tcgcttcctc ccacatgcac aaatattccg tcatattcaa gtgcatagta
    13141 aatcaggtaa ggcctggcgc ttcgcacagg tcctattctt tcaggctcct ttgcaaggaa
    13201 tatgcaaagg tacctggtaa atggatgctc cacttctact tcaaaaacta tttcagcctg
    13261 gcttatacca gcctgccatc ttgctcttgg atgattgtcg attgataagg caacaggtct
    13321 ttttaacaag tcttcaggat aatatcttaa tccgtccaaa ggtgaaagaa ccttatccgg
    13381 gtctatttct tcctcttcct cttccacctc aggttcttct tccgttatta ccggttcctc
    13441 cggctcttct tcaacaattt caatgttttc aggttccggt gtgactgttt ccctggtaca
    13501 cccgcttaga ataagcaaaa ataaaattaa tagtgtaatt ttcttcatgt cttccccctt
    13561 ggctgttact tgaatatttt taaatttttc ataatttctt tttcttttgc tctcatggcg
    13621 cttttttcgt cattttccat atggtcatat cccagcagat gaagcatgct gtgtactgta
    13681 agataaatca tttctctttc cggactgtga ccgaaatcat ctgcttgttc gattatcttt
    13741 tgagttgata taacaatgtc cccaagaatg gttacaccgt caaattcttc attcatggga
    13801 aatgacagaa catctgtttc actgtctaca tttctgtatt ctttgttaag ctcttttatt
    13861 tcttcatttg ttacgaatga aacactaatc tcaaaatctc catcacactc ttcttcggtt
    13921 aaaactgctt ccactgccct ttctattgca gcttcattgt ccccgtttat ttccatcata
    13981 tcctgcctgt cgtcaaacaa tacacttatc atttattttg cccctcatgt ttttcatatg
    14041 cattaacaat atcttgaacc agcttatgtc ttaccacgtc ggtagtgtgg aaaaacatga
    14101 agcctatgtc ttcaacttcc tttaaaatat gaattaccga ctttagtcct gatttcttgc
    14161 cctcaggaag gtctatttga gttatatctc ctgttattac ggcttttgag ccataaccca
    14221 aacgggtcag aaacatcttc atctgctcat ttgtggtgtt ttgggcttca tcaagtataa
    14281 taaaggatga atccagggtg cggcctctca tataagcaag aggtgcaact tcaataattt
    14341 gtttttcaac atttttcatg tagttatcta tacccattat gtcaaaaagt gcatcataaa
    14401 gaggtcttaa atacgggtca actttatcct gcaggtcccc gggcaaaaat cccagttttt
    14461 ctcctgcttc cactgcaggg cgtgtaagta ttatacgctc aacctctttt tttctgaatg
    14521 cattaacagc acaggcaaca gccagatatg tcttgcctgt tcctgcaggc ccgattccga
    14581 aggtaacatc tttctggtta atcatgtcaa catatctttt ttgtcccact gtcttaggtt
    14641 taataattct tcctgaagca gatgtaacta ttacttcttt taaaatttga ttgtaatcta
    14701 tttgggagtt tgatttattt gcttctatta aataaacaac cttttgatcg cttatttccc
    14761 cttcagcccg tattgtattt aatatgtcct ttataatctt agccgcagct tctgcgcttt
    14821 ccttttcccc tgtaaccttt aaggcgccgt tccgcactaa aatgtttaca tcgtaatagc
    14881 ttttaataat tttaatattt ttgtcatgta ttcccaatag tttaattaat tcatcctcat
    14941 ttgcaattaa aatattttgt tcaaataaat ccattattcc tccacatttt cctctgtctt
    15001 ttctttggct gttgggtaga ttcttatctt ttccccaata tcttcaatta cttctatctg
    15061 tgctattaaa taataaaatt cctcatcctc cgaaaagttt atgttggatt ttagaattct
    15121 tgagtcttca tcgcagcttt tcaaaagttc gtcatacata gaaactttca ttttattatg
    15181 tgctgtttcc ttatctattt caattttctt tattacttct tcatagtata cgccttttaa
    15241 aatactaata tcagctatat ttgaaacaag aggcacattt atttccctct ccctataatt
    15301 atagttttca taagggtctt tgtcacctat tattttaata ctatttccat taattatcaa
    15361 gtaataaaca gcattgcttt tatttgttga cacatttacg tttcttattt tttcttcctt
    15421 catttcaaaa ttataatatg ttttgccgta aacagtgcct tctgaaggca ccatcataaa
    15481 ttcttccgaa ttcttattct tgactatccc cataatcaat gtctggcctt cataaacaac
    15541 atccccctcc ttgaccacag cctggccgct tttagcaatt accttgttta caattgcgtt
    15601 tttcaaagcg attatgctgc ttggctcatt gctttttatt tcagccggtt caggttccct
    15661 ttcccttaca aaaataatca gttttgagcc ttcaacgaaa acttccacaa atttaaattg
    15721 aaaattatca taaataaggg tttccaattt tttagtttca atctgtgaga gggcaaaagg
    15781 cagttttatt ttattttttt ccaataaacg aattatctcg tttgtatttg gctgattagt
    15841 tcctatcact tccacatccc atatcatgga agtaagaagg tacagagata ttatcaatat
    15901 taaaagacca attgaaaagc ctcttctcaa ttttagctta ttaagcttaa gcaggactcc
    15961 ccttttgctt tcaggcttaa ctatttcatt gataaggtct gagtgtttta tatagtcttc
    16021 ataagaaatt ttgaatttta tctttttgtc tttcttttct acatcccata ccgaaatatt
    16081 tcttctccgc agtatattca acagcttttc cgagttcatg tcatcccggc ggatgaccac
    16141 atagcctctt attaaataca taagcttgaa tattaacata tttactcctt aatagaaaat
    16201 attatttctt tgatatttcc cttcactatt actttgtaac tgttgatttc ttctatctta
    16261 aactttttgc cttttattgt tatgttttgt tcattggtat ttatgctaac catgttttca
    16321 tcataaactg ttaatcctaa atggttttct ataattactt ttttattgcc gtgcattgta
    16381 agttcaaaat tatctgataa tgcattgttg ggtatttcca gctcatcggc caatttactc
    16441 ctgaaattgt taaacttact catgttaccc tccccggtca aatattgtct tattacaaat
    16501 tatgcgggta tgtacattac tatgaaaaat aaaaaagaac cccgcagggt tctctttcta
    16561 ctttatatat ttagccacaa tttcattgac atgctttccg tcagctttgc cttttacttt
    16621 aggcataatg aaggccataa gcttacccat gtcttttttg gactgggcac ctgttgcttc
    16681 aattgcttct ttcacaattg attcaaggtc ttcatctgac agttgaggcg gtaaatattc
    16741 taatagtatt ttaatttctt catttgtaag atctacaaga tcttgtcgac tgcctttttc
    16801 aaagtcaggt attgagtctt ttttctgttt aatctgttta gctattatat ctactatttc
    16861 agtatcggat aactctattc tttcgtcaac ttctctttgt ttaatagccg ctctaaccat
    16921 tgtaacagta tcttttttga ctttgtcttt agacttcatg gattgcttta aatcttccat
    16981 gagtttctct tttaaaggca tactttcacc tctttatcct cttgcttttt tatttttctt
    17041 tctacgagct gcttcagcct tctttttacg cttaacgcta ggcttttcat aatgctctct
    17101 ctttcttact tcggacataa cacccgtaag tgcgcattgt ctcttaaatc ttctaagagc
    17161 attatctatg gactcgtttt ctcttacttt tacctctgtc attctacttg ctaccctccc
    17221 ctcgtcttct actaccagtt gtcctaggac actagcgagt ttgaaatagc atttattatt
    17281 atattacata gaataattaa atgcaagtaa tttatgccat tgattcagat aatttctttc
    17341 cgcctaatat atgaaaatgc aaatgctgta ccgattgaca gccgtctttg ccgcagttgc
    17401 ttataaccct gtagcctgaa tctttgatgc cttccgattc tgcaatttta ttgattgctt
    17461 caaaaacttc tgctattata tggctgtttt cctttgtaat ttcattcatt gaagctatgt
    17521 gaaccttcgg tacagctaat atatgtacag gagcctgagg agccaggtct ctgaacgctg
    17581 ccatgtattc attttcaaat accaactgag aaggaatttc tttgtttaca atcttgcaaa
    17641 atatacaatc cattatttca cctcacatca cttaatttat tctccaacca aataatcgtt
    17701 ccttattcct ttaatatgaa ctttaattat ttcgtttcta acatttatat cactttttaa
    17761 caaaactttc aagtagtttg ttgtataacc ttcatataaa ttatcatcat tttgggtttc
    17821 tatcaggacc ggtaaatccc tgcctataaa tccacccata aattcataag aaattctttc
    17881 ccctagatca ataagctgcc tgcttctgtt aaattttatt gtaccggcta cctggggctc
    17941 catatctgcg gccttggtcc cttcccgcat ggaatacttg aacacgtgaa ttcgagaaaa
    18001 gtttatcgct tttacaaatt ccatggtttc attgaattct tcatctgttt caccggggaa
    18061 acctacaatt atgtctgtcg taataccggc agcgggcata aattctctta ttaaggctac
    18121 attgttttta tattcttctg cagtgtattt tctgttcatt cgttttaaga cactgtcgct
    18181 tccgctttga agggataaat gaaaatgatc gcaggtttta tccaaagccg catatctttt
    18241 catgaaatct tcatttatca ggttcggctc aatagaccca agccgtattc tttcaattcc
    18301 ctttatttta tttatatctt cgattaattc tataaggcag ttttcttctt taaaatcttt
    18361 accataggat gatatgtgaa tgccgttgag cacaatttcc ttatatccgt tttctgcaat
    18421 tctttttact tcctttataa tatcctcatg atttctgctt cttataggac ctcttacata
    18481 cgggatgatg cagtaggaac aaaattggct gcagccatcc tgtattttaa tattggcgcg
    18541 ggttttatca tgaacagtgc ttatttttaa ctcttcaaat ctttctcttc tctgaaaatc
    18601 gtcaatttca taatcctgtt ttatatcttt aagcttatcc ttaataagtt ttcctatatt
    18661 acctttatgc tttgtcccga taattatgtc tgcctgtgtt tccttattta ttttatcgga
    18721 attcaactga acgctgcatc ccactgcaac aaccaaggcg tcaggattaa atcttttcgc
    18781 cttgtttatg aactgcctgg atttcctttc gctttctttt gtgactgcac atgtgttaat
    18841 tatataaata tcactggctt cctcgggggg taaaacttta aatccctcat cctccagtat
    18901 ttctgtcata gcttcagttt caaactggtt tactttacag cccaaggtta tgaaagagac
    18961 tgtatattct gtattcgtat ccttattcat tcctgctcct aacaattatt tattgaatga
    19021 taatatcaca aattattttt aatttcaatc ccccctgctg tcacccgtca ccgaatgaag
    19081 gtccgcagca ttattataat gattgaataa tatgactcaa ccaaagtcca atgtcagcaa
    19141 aagatgaagg caccccggtc cccttgtcag aaaaacaagt tcagagcaag ctgaacttgt
    19201 taattaaatg cattttcaat atggtttaat tctttgcttt caagaattat ttctattaca
    19261 tcaaactggc aggggtaatc gtcctttcct ttcagcatga agtaataacc ggcagcactt
    19321 ttaagtcttt tttgtttggt atgggttaca gcctctcccg gatatccgta gtgtgcattt
    19381 ttacgccctt taacctcaat gaagtgcagc ctgttttctt tttgggcaat aatatctatt
    19441 tctccaaact tgcttgtaaa gtttgtttcc agtattgtaa aacccttgtc aagcagatac
    19501 ttttcagcaa ttttctcgta ttcaaagccc ttatttttat tatacattat cttttaaagg
    19561 ttcccagaac tcccctaata agtgaacgac ccacttctct tcctatggcg ctcatagctg
    19621 aattagcaac cctgtccacc ggggttaatc tgctgcttgc tttcttcttc aatttttcca
    19681 gttctttctc ttgtctttct ctttcctttt cttcttcttt gagtcttttt gcttcttcct
    19741 taagccttct ctcttcttcc ttggcttcca gctgaagtct tttttcctct tcctttgctt
    19801 ccagctgcag tcttttttct tcttccctgg cttcctcctg aagtcgcttt tcttcctcta
    19861 acctcttaat ctccagaagc tgctcttcct gtttcctttc tgcatttgat atcaacattt
    19921 catatgctga ttctctgtca actgctttgt agtatttctt ataaacagga gcgcttacaa
    19981 ttatttgatt tctcagcata tcatctattg ttccaaactg gctttgtggc ggaagtatga
    20041 aggcacgctc aactacagag ggtattcctt tttcatcaag gcaggaaatc aatgcctcac
    20101 cggttccaag ctcggttatt gcttccactg tgtcgaactc cggattttcc ctgaatgttt
    20161 ctgcagccgt aacaaccttt ttcctttcag caggtgtgta agccctcaat gcatgttgaa
    20221 ttctgtttcc taattgtccc agtatttcat ccggcacatc cattggattt tgtgttacaa
    20281 agtaaacacc cactcctttt gaacggatta aacgaaccac cagttcaatt ttatcaagaa
    20341 gctgttttgg ggcatcttca aaaagaagat gtgcttcatc aaagaaaaat accattttag
    20401 gcttttccaa atcaccttct tcagggaggt tttcatataa ttcagtaagc atccacaata
    20461 aaaatgtaga atataacaaa ggacttaaaa ataattttgc acaatggagc acatttatat
    20521 aacctctccc gtcattagaa gtttttatcc agtcatttat attcaattca ggttctccaa
    20581 agaagaaatc gccgccctgg tcttccaaat tcagcatatt tcttaaaatg gcgcctatgc
    20641 tttgagctga aatatttccg tatttcattc tgaaatcatt tgaattgtca cccacatact
    20701 gtgccattgc ccgtaaatcc tttaaatcca gcaagagaag tcctctttca tctgctactc
    20761 tgaatattat atttaaaaca cctgcctggg tatcatttaa atctaaaatt cttgaaagca
    20821 ttaagggtcc catttcagta acggtagttc tgacaggtat tcccccttca ccgtagacat
    20881 cccaaaattg aacagggaat cctttatagg taaaattatc tattccgaaa cgcgtaatcc
    20941 tctcctgcat attttcattg tcagtgcctg caattgcaat gcttgccaag tcccccttaa
    21001 tatcggcaat aaaaacagga actcccaaat cactgaagga ttcagccagc acttttaatg
    21061 taacagtttt tcccgtgcct gtggctcctg ccaccagccc gtgtctgtta gccatcctcg
    21121 gttctaaata tactcttttg tcactttttc ctatccaaat tttattatcg gcaaacataa
    21181 cataaatctc cttttataaa tttatctgct tcctttgtca tatggaatac cttctgctct
    21241 tggtgcctgt gaattttttg aagagaaggt aagcactatc aaggttgcta tatacggaat
    21301 catcttatat acttcatttg atatcggtaa tcctttaaga aaggggattc ctgagtaagc
    21361 tgaagcaaag gtcttcatta ttccaaagaa aaatgctgct acaaatactt tttttgtcct
    21421 ccattgcccg aaaatcaata ctgctattgc caaaaagcca tagcctgcaa cagttgcatt
    21481 aaaatttgta cttgtgggaa ctacgaatgt aagcccgcct attcccgcca atatgcctga
    21541 aataattacg cctgcatacc tgatacggta gacatttata cccactgaat ccgcagcttg
    21601 tgggtgttca ccgcaggccc ggagcctcag gccgaaacgt gtatgattta ttacaaacca
    21661 cgaagcaagg gctatcaaaa gtccaatata tgttgttata tatgcattct taaacagaat
    21721 ggggccaagt atgggaatat cccctaaaat cggaacactt cttatataaa atgtatccat
    21781 aaactgtatt tgctgaactc cttgaatcat ccttgccgta tacactgcaa aggcaggagc
    21841 cagcatattc agtgctgtac cgcttatggt ctgatcagcc cttaaattaa ttgcagcaaa
    21901 agcatgaaac agtgaaaata ttgcgcctgc cagggctgat accaatacag ccagcaataa
    21961 aaggctttga ccgctcatag aattctggaa gatatgaata aatccgattc caaaaaatgc
    22021 ccctattacc attattcctt ccaaagcaat attaactata ccgcttcttt cagagaacat
    22081 tcctcccaaa gctactataa gaagaggaat tgcaaaatac atagtctgtt gtgctataaa
    22141 gtaaaccgta ctcatttaaa tatcctcctc cctttgcact gtatcatttt cttcagtttt
    22201 agtatcaaca accggtgatt catttccggc acccatttct gtttgtatcc cttcttcaac
    22261 atccggttta accgggtaat catcatcaga aattacactt gattttaccg gttctttaac
    22321 atcctgggct ttctcttcaa caggtttgaa taaaagcctt ttgataatct gtttaaacaa
    22381 aagggcaaat gccccgcagt aaataataac agcaattatc atatctatta tttcagggac
    22441 gaagttgtaa agctgtatat taaatcctcc cacttccaaa tgtgctataa ataaaccggc
    22501 aaggaatact cctatgggat gtgacattcc taaaagcgct acggaaatac cgctgaatcc
    22561 ttccggtgcc agcacatcca aaacatgaat atactttcct gagcctgaaa gatacaagag
    22621 tcctcctcct aatcccgata aagcacctgc aataaccatt gacagtatta agcttttctt
    22681 ttcattgatt cctgcatacc tgcttgcttc cttattgctg ccgcaggctt ttaattcata
    22741 accaaaggtc gttttctgca ggattatata aataactata acagctaata ttactataaa
    22801 tattccaatg ctgacattgc tggtattaaa aagcttatcc atgccagcct ttggcagtac
    22861 tgcacttttt aaaaccggtt ttgtctggtt tttcaatgaa tcgtatactg ttttaactat
    22921 tgacatatta acaaggtaca ttcctatata attcatcatt attgatgtaa ttacttcatt
    22981 tacattaaaa aatgctttta gaataccggg acctgaaccc cacacagcac ctgcagccat
    23041 agctcccaat aatgcaacaa gccaatgtat tccttcggga acaaaggtcc acttaattcc
    23101 tatataaatt gctacaaaag cacccattgt aaattgcccc gatgccccta tattgaataa
    23161 tccggtttta aaagcaaagc ctacagataa gcctgtcata ataatgggga ttgcaaagta
    23221 tattacctgg gccaggcctt ttgtgccgtc tgtaaatcct ccttttaaaa tcgttaaaaa
    23281 agcaggaaag gcatcttctg cattactaat taaaagtatg acaagaccaa caaggagtcc
    23341 tgctaaaata gccatcaaag aagataaaaa acttgtaaat tcttcatact gcaaaagtct
    23401 agttcttctc atacttatca ctcctttttg atccggccat ataaagaccc agttcctcta
    23461 cagtaatttc tttagggtct aaatccccta caatttctcc ttcataaatt accaggattc
    23521 tgtcgcttac atccattacc tcgtcaagtt ccaaggatac taaaagcact gcctttccat
    23581 ggtctctttg agctatcagc tgcttatgga tatattcggt agcgccgata tcaaggcctc
    23641 tggtaggctg aacagctatt acaatatcag ggtccttgtc aagctccctt gcaattatta
    23701 gcttttgctg gtttcctccg gacatgtttc tcactattga gtcttcatct cttggacttc
    23761 ttatatcata ttgttttatt aattttttgg cgtattcttt aacagcatta aatttaataa
    23821 atccgttttt ttgaaaacgg ggctgaaagt aagtctgcag cacagaattc tgtttaagat
    23881 caaaatccag taccaggccg tgtctatgcc tgtcttcagg tatgtgtgac attccgtcca
    23941 gacttttttt ccttaccggt tccttggtaa tatctttacc ctttattaat acttggcctt
    24001 ctttaattgg tattagtcct gttattccat ataccagttc ggattgtcca ttgccgtcaa
    24061 tacctgctat gcagaggatt tctcctgctt ttgctgaaaa agaaacatca ttgactatat
    24121 ttttctttga attttttgct ttaacagtta aatttttaac ttccagaata gcattccctg
    24181 tttcgatatc ttccttttct atgttaaagt taattttacg ccccaccatc atttctgaca
    24241 tttgttcctt ggtggtagtt tctacatcta cagttcctat atacttgcct tttcttaaga
    24301 ctgtgcaccg gtcagccgct tctttaattt catttaattt atgtgagatg aaaacaattg
    24361 attttccttc acttaccagt tccttcatta ttttcattaa ttcttcaatt tcctgcggtg
    24421 ttaaaacagc tgtaggctcg tcaaaaatta atatttcgct gtctgaatac agcatcttga
    24481 gtatttcaac tctttgctgc atgccaacag ttatatccga aatatatgca tccgggtcta
    24541 tttttaattt ataacgttca ctgagggcta ttaccttttt tcttgcgtca tctttcttta
    24601 aaatacccat gtttacggtt tctctgccta aaataatgct ttcaagcacc gtaaaattat
    24661 gaacaagttt aaagtgctgg tgaaccatac ctattttcaa agtgcttgca tcgtttgggt
    24721 tatttatttt aaccggatta ccattaattt ttataccgcc tttttctgct tgatataaac
    24781 cgaataaaac actcattaaa gtagatttac ctgctccatt ttctccaagg agcgcatgga
    24841 cttcaccttt tttgacatta aaggtcacat catcaagagc tttaaaatta ccgaatatct
    24901 tggtaatatt gttcatttca attacgtact ccatctaacc acccgcctta attataaagg
    24961 ttgcttaaaa agcttacgcc ctaaaagcaa cctttgaata atttattact gaataacttc
    25021 tacttgaact ttacttaccg gaatatctgc tgctgttact tcttcagcag gttttcctgc
    25081 atcttcaaca acagctgcat cattcattat ttctatttgt tttgcaacta ttttaccata
    25141 gatagcatca taatctgcct gtgtgaattt ttcaaatctt gaattttcca ttggcagctg
    25201 tactccttca acagttgcat caagtgaaac tgaagttcct ccggggaaat tgccagcata
    25261 gtaggctgtt aaagcatcgt atacagattt tgaaaggttc ttcattgatg atgtaataac
    25321 tgtttcagat tctgccgact ggtcaacgtc aacacctatt acttttgttc ctgctgcttc
    25381 tgcggctttc ataacagagt tacctacagc accaccgcaa gcaaatatta cttctgttcc
    25441 ttcattgtac catgatgaag cttttgcctg gttttcaggg gaagcatcaa agtttccaac
    25501 ataagtatat ttaagttgaa ttggttcggt taaacctaac tcatgagctg catattcagc
    25561 gccttgaaca aagccgtatc cgaaacgaac aactgcagga acagccattc cacccataaa
    25621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cctccggaag cttttatttt
    25741 aacattttgt ccaatatgct tcttgaacaa cagtacatca tccattgttg caccgcctgt
    25801 accgaaacct gttgatgtct tgatatagtc tgctcctgca tttgttaccg catggcacat
    25861 ttcgattttt tcttcttctg taaggtagca tgtttccact attaccttta aaatatgatc
    25921 tcctgccgtt tcctttattg tgcggatttc gtcctcaacc tttttatagt ctttgttttt
    25981 cacatcagat atattaatta ccatatcgat ttcattgaca ccgtctttta gggcttgtaa
    26041 acattctgca accttggctt cagtagtcga atatcccaaa gggaaaccta caacggtgca
    26101 aatattaacc ttatccccgt attcatcatg aattcttttt atataattag gtggaatgca
    26161 tactgaagct gtctgatatt ttactgcttc atcacataag gtttttattt cttcccatct
    26221 ggcataagct tttaataatg tatggtctac atacttcatt aattcatgaa tatccataag
    26281 agcctccatt cctttatata aaataagttt agcacaaaat ccctaattcg tcaattagtg
    26341 atgtcaatat cttaggttta ctttctatag gatttcataa tatttgaact tcttttccta
    26401 tagagaataa atacaaatag cactccttaa cctttatgcc ggttattttc tcaagagctt
    26461 ccttgtatag tattacctgg tcctcgtatt cttctttaag cttttggact tgagcagcaa
    26521 catttttcct ttcatcaatc ctgtctgttt tataatcaac tattaccgct tcattgtcct
    26581 ctatgaagta agagtcaatt ataccctgga caagaatcag gtcttcttcg ttaaggcttt
    26641 ccaatacatc atttgccttc tttcttaata caaaaggtac ttccctgtaa accgaatcag
    26701 aattaaccat tctttttcca atggcggttt tgaaaaattc ttctattttt tctgcatagg
    26761 ttcccgctgc aattttggct tcatcttctg taagaagttt tctttcttcc attcgtttta
    26821 tggtgttaac tatgccgtag ctttttaaat cccccttcag gtctaaatgc tccagaacaa
    26881 aatgaagcag tgttccaatt tctgcacctg agatttcttt atccagcagg aatttgtcat
    26941 cattctcatt ataattgaat atatcaacca gggttggaat tttgtatctt agtttggaaa
    27001 actccttatc ctccaagttc ttaagctctg ttaccgagag cttagtgggg acatttaccg
    27061 aagctgcata tgggtatata tagcttaacc gcctgcctat ttcccggtaa tgagggctgt
    27121 tttccatttt cttgaactca tttaattctt taattctgct ttcttttaaa ttgaactcat
    27181 catttacgct catgcttaat tcatactgtg atagcagttt taaattccag ccttcctgct
    27241 gacctatttt cattatctca ttgaattttt caaggtttac cttctcaaat aaacagccgc
    27301 aaatccagtc catgtatgaa agacccttgt aaacaaagta ttccgagtga ccccttctcc
    27361 aaattctctg cctttgctcc aaattcccta cagcacctgc cattataaga cggtcaatgg
    27421 ctctggtcat tgcaacatac aagactctca tttcttccga aatattctct tttttgatct
    27481 tcagccttat tgcagttctt gccagtgtct ctttttctat cctttcatca acatttacat
    27541 actttggagc cataccatag tccttgtgca tcagtatttt atttctcgta tcctgcaggt
    27601 taaactgctt attaaggccg caaagaataa caacgggaaa ttccagaccc ttggacttat
    27661 gaattgacat cagccttacc acgttgtcgt tttcccccag tgtttttgcc tgccccctgt
    27721 catcagcccc ctttgtgacc ttttcaatat atcttaaaaa tttgaacaag cctctcattg
    27781 atgtcttttc aaatttatat gccaaatcag ccagcatcct taaatttgcc tgtctcatct
    27841 ttccgttagg gagggctccg gcaaaattgt aataatttgt ttccatcaag atgtcccaga
    27901 ttaattcatt tagattcata tacctgctct tgtaagacca ggaatatatt ttttctataa
    27961 aatcttttaa tttagagagt aattcagaat gatcaccatc cttttctcct tgtataaatt
    28021 tacccgcagc atcaatgaaa tatttttcct tagggtttcc aagcctgatt ttcaataatt
    28081 cctctgttgt aaaattacct atgggagacc tcataaggct caaaagaggt atgtcctgtc
    28141 ttatgttgtc aatcagcctc agcagattta ctacaatttt aacttccaat gcttcgtaat
    28201 atccctttcc cccgtcataa taaaatggaa tatcttcttt attaaacctt tcttcaaata
    28261 tgcctgccca gtttgatact gatcttaata aaattacaat atccttatat tcaatttttc
    28321 tggtaccgga attgccggga attgttattt cttcctttat cagttccttt attttcttcg
    28381 cagcaaacaa agcttccagt tcagcggttt tcatggattc aatctcaagc tctatttggc
    28441 tttttgcctc tttgaaatct tcgccgtcct cttcatcgcc atttttttca tatcccttta
    28501 aagaaacatc cttttgaatg atattaagct caacaggagt attaaaacaa aattctgttc
    28561 ctgtattaag tgctgctttt tcatcgtact ctacttcccc taattccttc gacataatat
    28621 ttttaaatac aaaattaaca gcctttaaaa tttcatctct gcttctgaaa ttcttattta
    28681 aatcaattgt cctgtttatt gtttcatcag acagcttact gctgtcccgg gtataggctg
    28741 tatatttata gttgaaaatg ctggggtctg ccagcctgaa tctgtatatg ctctgcttga
    28801 catctcctac catgaacaaa ttattttctc ttcttatctg ctcaatgatt gcttcctgga
    28861 gactgtttga atcctggtat tcatctatga aaatataaga atattttttt ctgtaatagt
    28921 cagcagcttc tgaaggtacg aattctcctg tctccttctt gtttcttaat atttttagtg
    28981 caaaatgttc cagatcgtta aagtcggcta tggacttttc tttcttcttt tctctgtaat
    29041 ttttgtctgt ttttattatt aattcactta atgaggaaag agagccgtac atttcattga
    29101 tttcccatgc gtacttgtca taatccaaat catatggaag gacttccttt atagaagcaa
    29161 taacagcttt gtattgtgct ctcatgctgc tattgttctt agaacctgca acttcaatct
    29221 gcttttctat gtctacatcc ggataatctc tttccttaaa ggaagctgct ctgggcgcat
    29281 taaagtcctt gagggctgtc atgaattcct tcatatccct gtcaataaga gctatgaggt
    29341 tgtcaacaat gtccgtatcc ttttttaaag tatccaggta aagggcagga ccgccaggct
    29401 ccttgcatat ttcaactgcc gtattgatat atcccttggc cccgttcagc aaaagctcaa
    29461 tatagctttt tatttcatca agccactttg aatttttcaa ttcttcacct gtaatactga
    29521 gttttcttac gctttcttca agccatgaaa aagggtcatg gaagcttaaa ataaacttat
    29581 aagcagattg aattattgag cttagctcat catctcctct gtttccggtg aatccttcaa
    29641 ccagtttttt gaaattttca tcatcgttga ttttttcata ttcttcttcc agtgcttcat
    29701 ctatggattc ctgcaaaagt atgcttaatt cactttcatc acccaccctg aaatcagggt
    29761 caaggtcagc caggtggaag ttcttcttta ctatatccat gcaaaatgag tcaatggtgg
    29821 taatcattga cctgttaaga agagatagct gccttcttaa atgccttaca tccccttccc
    29881 tgttttgaac agctttgaca agggagcttt gaattttctt cttcattccg gcagccgccg
    29941 ccctggtaaa tgtaactacc aaaagcctgt ctatatcttc tccgtttttt atcagtgtaa
    30001 ttattctttc aaccattacc gcggtttttc ccgatccggc tgcggcagag accaatatat
    30061 ttgaattttt aatgtcaatt atgcgttttt gttctttagt ccaattcatt gtatcctcct
    30121 tccgcttctg ccgctgttaa gatttcttgt ttttctttag aagcaggata tttcgtgcct
    30181 atgggacggt aattatttcc caggctgctg tcaaactgac agatggaaat ataatcgcag
    30241 taagtgcagg gtgttttccc gtccggcttc ctgtaagggt caatatttat attcccttcc
    30301 aaaatgctcc ttgtcatgtc tgcacacttt tcataaacta tatccattac attcttgaat
    30361 tcttcttcgg ttaatgcttt agtccagtct tccctaatgt tcccgtcttt tttaagagct
    30421 gcctgtatga cctttgaagt gctgtcaatg tttttatcca taagcttaac gacctccttg
    30481 tcttttaaaa catagccctt aagttttaat tttttgaata tttcttcctc cggatctttt
    30541 aatttgttgt catctgtaag cattgggtca ttaataaagt aataaaacac tccccccaca
    30601 tgagttttcc tgccgaataa ttcttccccc ttgtcaatca atgcctttaa atacataatc
    30661 aactgaacct gtatgccctc tttggcgtcc tccaggtcaa cttcatttgg agaagatttg
    30721 taatcaataa tacttacata cagtttatcc ttgttttcaa aaacatccac cctgtctatt
    30781 tttcctctca gctttaatac ttcctttatt ttttcagtat caacagatat ttcaataggt
    30841 ggaacgactg tctccgcctt ctcatttctt tcatcaatct tgagctccgt ataggcaggc
    30901 ctgaacatgc ttgaattcaa ctgacctgca agagtccggc aagttctttt cataaccctt
    30961 tttatctttt ccttcatgta tttattcctg taatttgctt caatagcagt aagcttgtct
    31021 tcctgattct ttaaaacttc atttgtgatt ctgacagctt cctcttctgc cctgtccatg
    31081 tcaagaagac ttaagtcctt acactcttcc tttattttat taatgaattc ttctacaacc
    31141 gagtgatata tatcgcctaa gtcataaaat tcaatttttt gaacaactct ttctcttggc
    31201 ttaagcacat tttccataaa atacttaaac tggcattggg cataggtttc aaatttggaa
    31261 actgtcattg tcatattatt ttcataaatg ctttttacgg tttcaggctc taaagtatcc
    31321 attgagtttt tatattttag ggctttgttt attatataaa agttatcctt gtcttctttt
    31381 tcataccagg cgtaaacatc cctccaaagc ttgtccacct catagccatc gatataagat
    31441 ctcatatttt caacaaaata atcataggta ccaccatagt ttgaaaccga ttccagttca
    31501 tctcctatgc tcaggtccga gccagccttc atatttacga aaattttctt caatatatct
    31561 atatacaatg atggctgcag gctttccccc gtctccaagc ccatgggata agatatgtaa
    31621 agactttgtg aaggacttga aaacagcttg taatgcatgt gtttttcttt aaaggtgtcg
    31681 taatctgccc cttttgtaag ttttacatgg ttttcaagaa gaagttctct ttcttcattc
    31741 aataaaagac ccttctcggt attttttgca tccagattgc tgtcatttgc ccctatgata
    31801 aacaaagcct ttggttttgt aacagcaatt ctgttaattt cgccaacttc caccttgtct
    31861 attgcaggag gtattatact gacttcaact tcaccaaggc ctgcttccag catttttctg
    31921 tattccctta ctgaaatctc tgtatctttt ccaaccagca taagctgttc gaatacttcc
    31981 atgacataat tccagcactg ggaatattca ctggcttctt cgaatttatg catggcatta
    32041 aatcctttga cagccttctc catcttttct tttactttat gttttttaag atacttaaat
    32101 attagttttg taatttcatc agccctttta aggttttgta tttcttttct ttcagtttca
    32161 aaatcaccgg caaatagttt cctgtactct tccatttcat tttgcttggg aggtttttga
    32221 ttatcctcat ccttaccaga agttttaaat tctctgaacc acttttctcc ttcaatgccg
    32281 tactccaggg cataattctc caaaagatga acctgtttga tgcttaaagg agaaaaaccg
    32341 gtttttaagt attcaaaaac attgtcatgc ttgaatttcc aaataaacat gtcaaaaatc
    32401 gaaagaatgt acttgcttaa aggattgttt aaaatatctc tttttacatc caggaagtat
    32461 gggattttgt attgggcaaa tattctttta acattcgttt cataggtatc catgctgccc
    32521 agggcaagct tgatatccct ccacctgtaa cccttatccc gaaccaggga tattatttct
    32581 gccgctgttt tttgtgtttc cctgtatgga ttcaaagaag agtaaacagt aattttaact
    32641 gattggtttt catattcttc aatgtttaag gaaaacatat ttttttcaac tgcttttatt
    32701 tcaggggaag aatacatgtt gtcttttaca gatataattt caatttcgat atcaagtttt
    32761 ttcaagttaa tatatgtgtc atatataatt ttaaatgctt cgtagtcgtc cttttcatcc
    32821 ggctcaataa aatatgagga gtcaatgttg agagaagctg ttacggacct ggaattttca
    32881 caaagggctt ttatcaaatg gagtctttga taattaaagc tttcaaatcc gtcaatccat
    32941 attttagact tccttatata tgctgaatcc tttatcaaag aaatgcaaag gttagttttg
    33001 tcctcttcat caaaatattt tccttgactt cttttgataa actcagaata aatcaattca
    33061 atatcggata gcttacgcat taaaatttcg tctgtcactt gcagggctgc attttttaat
    33121 gattggctgt caaccaggtt ttgtttgaat tcagctgtca acttttcaaa ttcttttaaa
    33181 aatccacttt gtttacagga gttgccgaat atcttaagtt tgtccttatt tccttcaaat
    33241 atctgcttaa gaagcatgat tttcccgaaa acgcttatat cctgtacttt cagaccgcct
    33301 acttcctcta aaaccttcct ttcaagtctt ttaaagctta aaatttctat attcataatg
    33361 cccggcagcc cgaacctctc aataatgctg ctttcagcct gatatgtcat aagctcgggt
    33421 actataagta ttaaattgtc ctttttatct tcagaccggc ttttaatttc attataaata
    33481 aatgatgatt tcccggtgtt tgctcttcct gccaatattc ttaaactcat aagaccacct
    33541 tgtccgatta attacattat aaaacccata tattattata ataaattata ctggaaaccc
    33601 ccttctaatg caataaaata taacctttat gaatcaggtg caaataaatt agcggatttg
    33661 cctgtaaacc caggactttg actgaaagga gaaagttcat acgaacttta aaaaccgtta
    33721 gacatattat aatccaggag ggttgaaaat gaataataat tgctatagtg cagacataat
    33781 catgcatgaa tacaataaaa catttaatta taacaatact gagatgctaa agctcacaat
    33841 caaatatcca caaataagtt tgaataacaa acaggcagag aatataataa atgctcaaat
    33901 atacatggaa gtaaacaatt ataaaaaata cgcaaaacac ttgtttaagc aggcaatgca
    33961 agcatacgcc cggtcaaaaa ctaccggcta tccttttttg ggatatgagg catatatgga
    34021 gtattttata acttacaatg aaaattgttt cttcagtttg tattatgaca ggtatgaatt
    34081 tacaggagga gcccacggca ctactgtaag agcttcagat acctgggagt tatgttgtgg
    34141 aacctgtctg tctctttaca gttttttccg tccggacaca gattacaaac atttattgat
    34201 taatgaaata attaggcagg cagaagcaat acagcgaagg cagaatatat attttgaaga
    34261 ctacaaatcc ctgattatta aaaattttaa taaagacagc tattatctgt ctcctgaagg
    34321 gataacaatt tattaccagc aatacgacat tgcaccatat tctacaggaa tagtcgagtt
    34381 taccgttcca tactcagtaa taggctggaa gcccaattgc tgatacttaa agccggcttt
    34441 cggattttaa ttaatggtct gacttttttg aggccggctg ttatttttta atttgctgtc
    34501 atccttttta tggcataggg caggaatttg acaaggtctc ctgacgtcag tgaatactct
    34561 cccagtattt ctgctcctat gtcaccagcc aggccgtgga ggtacactgc tgctctcaat
    34621 gcattttctg ttttaatccc ttgaccaatc aaggatgcag ctgcacctgt caatacatct
    34681 ccgcttcctg ctgttgccat tccaggattt cctgttgtat taatataaat tttgccgtca
    34741 ggacatgcaa taagggttct tgcacccttc aaaagcaaat aaacattgta atccatagaa
    34801 aattccatgg atttttttat tatatcagtc ttaaagtaat ccatatcctt attaaggagt
    34861 tttaccattt ccccgtaatg gggagttaaa acaagaggct gactatgatt ttttaaattc
    34921 ttcaaatcaa gcttgtttat tccttcagca tctattacca ggggcttttt gcattttttc
    34981 agcaaataat ttaatatgtc agaataatta tttatatttg tacagcctga gcctgttgca
    35041 accacatccg aataatcaat aatttcacgt tgaagctttt cgaaggaaat gttatctgca
    35101 tcataggtgc atgtaatggc ttccaatgac atagattcaa caacataata aatctcttcc
    35161 ggtataaacg cctttacaag tccgctgccg catcttagag ccccgttaag gtttaataca
    35221 caagctcctg ccatgccctt tgagccggat acaaatcccg ccttgccgta ggttcccttg
    35281 tgcgaattcc tgctgcgttt tatcagtata tttttaacat catcaagttt tgtcaaccga
    35341 acattatcca gttcagaaaa aatatcagct tcttctttaa ttcctaaaat ataattatct
    35401 aaatctttca tgttcatatt cccgcctcat ggtccatccc cgttattttt cactcttatc
    35461 tcctcagtaa agaaccttca ataaatttat aaatgccctc ttcgagcttg tcaatatcat
    35521 ttaagttatc atcacctaca tatattataa gacgctcctt gtaataatct gatgaaaaag
    35581 aaaattgata cattgtaaat atattatcca tacagtatgc ggcaattttt tcattgatat
    35641 cattttttat tttgccttct ttttttgcct tttcaataaa ttcccataga atttgcggag
    35701 taattatttc tattgtattt gacaatttta ttgacatatg tgaaagggct tgggtggtta
    35761 tatcgagata aatcctgttc aaatgaggat gtctgagtgc aaactctctt gatgccttaa
    35821 tcatgccttt tacacaatca aatatatcac tgctctcatc tgatatattc tttaagatgt
    35881 cgtccatgag ataataagcg ttatttacga tggacaaaaa taaatcttct tttgatgcaa
    35941 aatagctgta catggcaccg atgcttatct gtgcattcct ggatatatcg ttgatactgg
    36001 ttgcattata gccttttaag gcaaactcct ctatagctgc tttcagtact tgctgtcttc
    36061 tttcctcagt tgttttatca aatgtgtcct tgtgaaattt atttttttcc atgttgcatt
    36121 cctcaatcca ttccgtttat cattctgaag gtttttatat tatttttata taacagttta
    36181 aatacattga aatttctgtc atgaacttcc ttgtttaggg gatttggata atatacgtct
    36241 ttaaccttta taagattatt tgcttcttca atgtgatcaa gcaatcccat tccaagggca
    36301 gcaattactg cagctcccag ggctcctgca ttctgtgtgt tatctactgt ttcaattttg
    36361 catcctaata catctgatag agtctggcag gttaatggtg ctaatgctcc tcctcctgca
    36421 aaacgaattg tatcggaagt tttgaccttt ttcttctgtt cttccagcat ccaccgcaaa
    36481 tgataggcta ttccttcaat aaccgcatga atcatatccc gcttgcctgt ttccaggctg
    36541 atattgtaaa acattcctct tgcattggag tcttcaaaag ggcagcggtt cccatgaagc
    36601 cagggagtga aaattactcc attgctgcct gcaggtacat cctttatttc ttccatcata
    36661 aaatcatata aactaacgaa ttttgattct tctgaatcag atacacttac tttttcaagg
    36721 taaatattta tttcatccaa tgccaggtgg tccttaaccc attcaagaca tttgcccgat
    36781 gtttccaatt cggcaaaata gttgtagctg tcatcatctg cacctacaat cgatgccatc
    36841 atggaagtca ggtctattgc cctcttcttg acaacagttg atacccatgc tgatgtaccg
    36901 gaataaatgt atgtatttcc aattcctgca gcacctgccc ctattcctat caaagaagca
    36961 tctcctccgc ctccgtatac gggagtacct tcctgcaatc cgagtttctc tgctgctttt
    37021 tttgtcaacc ctccaaccat atctgaagat ttcaaaattt ccggcatgtg ttcataatta
    37081 acacccagca tcttacacat ttccctgctg aagcagtttt tgccatccct tacatcatac
    37141 aataaggtgc caaaagcact gtcttttgtc ataacaaatt tgtccgtaca ttgcaggata
    37201 agatattctt taacatccag ccatttatat accctcttaa aaatatccgg ttcatttttc
    37261 ttaacccact tatatttcca tacagggtct tttgcactgg cagcaactgc acctgtaatt
    37321 cttatggatt ttaaaagttt gaaaatatta agaccggaaa tcctgatacc tttaccgatt
    37381 ccctctttca tttcaagata tgccctttgg tccatgtagc tcattgtccg ccgcaatgcc
    37441 cttccgtctg tatcaacaag aaccagactt tgcatctgtg aacagaagga tataccctga
    37501 atttcttctt tggttatacc atatgaaagt actttttgtg ttgtactgca catggcattg
    37561 tacaactcat ccgggtcttg ctctgcacct ccattgttgt ctatatacag cttatagttt
    37621 tcaacggcag atgcaatcag ttctattgtg tcggaaatct caaaaataca tgttttaata
    37681 ccggaagtac ctatatcgta agtaataaca taaacaccca tattacctca catccctttt
    37741 tcattcatct tttttgaata tttcttcagg tatatgaacc tcatcagcat gctgtcaaac
    37801 cggctcaaag gtttagcatc tttaaacaat gctttgcaga tttggattat gcaatttttc
    37861 tctaaaatca tttctacggc ggatgcatca tcttttgaaa ctgcacagca caaggctccc
    37921 ttgtttttaa taaggacagc tgaagaattt tttaaggctc ctgctatgtt aacaggatta
    37981 tttataacag tttccacctt tgttccgact atctgtgcaa aatcatccag catcggatag
    38041 agtgtaatgc cggcacttga tacatttaaa atatccggag cataagtatg gattatataa
    38101 ttaatatttt tgtgctttaa atatatttca ttatgaatcc ttgcatcttc atggtcaaat
    38161 gaaccggttt ctccatgctt cacataaata tactccttac cttcagcctt cattgtaaat
    38221 ccatcgtcag tacgccggct gtaattacaa acagccggtg ggttagtggt caataaactc
    38281 tgctttatct tttcgagttc tttcttcttg tcagaatcca ggtttggatc tttttcggcc
    38341 ttataatatt gttttgcaat atagtccttg caggcatcct ccagaacctt agcagtttga
    38401 aaggcttcct cctcatcctg cccgaaacaa attgccccgt ggtttttcat aatgattgca
    38461 tttccatatg agcggtttag agctgccctt atgtagctgc ttaatttttt tgttccggga
    38521 agtgcatagg aggcacaagc tatatttcca ggcacggaaa aatcagtgtt tatactgtct
    38581 aaacccatgg cacttaatac agaagcatat ttctgatgag tatggatgac aaagtttata
    38641 tccttattgt gtttatatat ttcagcatga attctcttct cagaagtagg tttaatactg
    38701 ccctcatagc ttaaattttc aattgaaaca accacagttt cctccggtgt caatgtcaaa
    38761 taacctctcc cgctgggagt agctgcaaaa tgactgtcgc tgatccggca gctcacattt
    38821 ccccaggtgc gtgcaataag tcctgtgctt accagtctct tgccggcact tacaactttt
    38881 tccctggctt ctctctcatt catgacaggc tccttatttt gtttattcaa ttatcccgat
    38941 atttttaaat actctttcaa acctgttaaa tacatcgtct atcatatctt catcataagc
    39001 agcactagta tataatctgc tccctgccaa tgttatgata ccttctgcca tgtatgccgc
    39061 gcccatatgc tccatttcct tttttctaag ggaagtcttt ttgataacat ctgaaattgt
    39121 ccatggtttt ttccaatcaa tgaagaaatg agttgtgccg actgtttcca aatggcatat
    39181 agacccttgg ttaaatgcca caaatggcag tttgtatttt tctatcagtt tctccaaacc
    39241 ggctgtgagt ctgtctccca ttaatcctga tttttgaaca gcatttgttc tttctatttc
    39301 gcataatgta tagtatcctg ctgtacagct taagggattg gctgccagag tcccgcctac
    39361 cagcgccttt ttttgctttg tccccccggc aattcctgct gaaagatatt tcatatattc
    39421 tttcttgccg ccgattccgc ctgcaccggg atatcctcct gcaataactt ttccgaatat
    39481 tgttaaatca gggctcacgc caaaataacc ttgtgcccct gccataccca ctctaaagcc
    39541 tgttacaacc tcgtcgaata tcaataaggc tccatacctg cggcagagcg attcaacccc
    39601 tttgttaaaa tccttatcaa ggggagttgt tccgctttca ggtcctaaag gctcaatgat
    39661 tacagcggct gttgaaccgt gcagtttgtt cattttcagt gttctttcca gatcatttaa
    39721 atcatttggg aagaattcct gtatatgttt aaatacatat cttggaacac cgcttgcctg
    39781 tgtccatttg gagcccggta tccgaatacc gtatgcaagc tggtcgctcc atccatgata
    39841 ggctcccccc atcttcacta tatttttgct gccggttgca agacgtgcaa ttcttatggc
    39901 agccatgcag gcttcggttc ctgacccaag catcctgaac atttcaacgt ttttcatgct
    39961 gtcggatatt ttttttgcaa tcttgtattc atattcatgg aaaagacctg tgctgggtcc
    40021 gcattcattg agcagttcaa ttactttagt acgaacttca acaggattgc ttcctaaaac
    40081 agtaggtcct cctgcctgaa gaaaatcata gtatttattg ctgtcaatgt catataaata
    40141 agctccctct gcttttgtaa atactaaagg aaagggatgg ttaaaagcca ggttgtgctg
    40201 tactccaccc ggaatcaaat cttttgcttt attaatcata tcttttgatt tcaaacattt
    40261 cctgtcgtaa tactcgtctt catatttttt taaagcctcc ggttttattg aataaatcgg
    40321 tttcgagatg agttcgtcaa gctgtttgtt tattttcccg gcatcaggat atgttgaaat
    40381 agaaaaactg ttgtccatgg cttcctccaa tttttaattt atgtaaatca ataatcactc
    40441 tattaatttc tttttagcca gttcgtttgt atcaattgat tttctgaata cataaaacct
    40501 ttgatacaaa aactcaagaa taaaattgct caccattata agaattgtaa caaggtactc
    40561 attccacata agcgtatctg caagataatc tcccagaaat gttgaaacag gtgtaaatac
    40621 caggtagaaa agaaaagttt tgaacatggc ggaagataca tttgccgaag aacggaaagt
    40681 ataacgcctg ttcagtgtaa aattccacag taccgataaa accaatgctg tcagataaca
    40741 cgcccagtag ggccagcctg tcagctcatt aagaagtgta aatgatacta tctctattat
    40801 tccggcagat aaggagaata gggtgaattt tagactccgg attgtttcct tttttatgtt
    40861 cttaatatcc ttaatattgt ttgtatggtc ttcttcaggc aaaccattca ttacttacct
    40921 ccgctatatc gagcgaatgc tcactctttg ttgctttaac tatatcaccc ttattttccg
    40981 gtgtcaacgt tttcataata tatttaaatc atcctctgtt ttataaaccg ttagaattat
    41041 atcatgccat acatgatgaa accaatggta ttaattgaga gattagcgct cacatgcact
    41101 atccatgaat tatataagtt attgtttttt agattcatcc agttaaaaaa taaacctgta
    41161 aaaaataagc ccaggataaa aactatatat atcactatat gaaaccagtt tgccagtata
    41221 gaaacatgat aaattgaaaa agcaagtgcg cttgttaaaa tagaatattt ataggatgaa
    41281 tgtttcccta aagttaaaaa accgaaacct ctaaaaaaca tctcttcaag cattgaatta
    41341 acaaaagaaa tataaagggc tacaaaaata aaattttctt tattaacatt tagattatta
    41401 ttaagctgta tagtaatatt atctaaatct ataaatcctt taatgataaa ataacctatt
    41461 aatataacga agtaaacgct gatgcctaat acggctgcaa taaaaatctg tcttttgtca
    41521 ttgataatga agtattcctt gaatttaaaa ttcttatcta ttaaggaata gcacaatgga
    41581 agtcctgcaa ataataatat tttataaact gatttaaaaa tgtatgcagg tttaacataa
    41641 gtttctatca aatacatgga taagcatgca aatatcagga tacatgttat tattattttt
    41701 ttcgcactat ttcttatttc ttttttcata tttattccat cctgaatttc gttatattca
    41761 ttatattatt tgtgggattt aagtcaatag cataaaatac actaaacaga caatagatta
    41821 agagtaaaat aattagaaca cttggctgca tttgcattca taaattttac aaaaattgta
    41881 aaataatttt tttgttattg acatttagac atgataagct atatactgtg ttttaataat
    41941 ttaataaatt catcaagagt gacggaggga cctggccctg tgaagtccgg caaccaatct
    42001 gagtatatgg tgctaattcc agcggtatta accggcagat gaggaaaaag acccgtgtct
    42061 tccccgttct gtggaagatt ttttaatttg ggaaattcaa tttattaaaa attcaataaa
    42121 aaattttaca aggaggaata atatgaatat taaagaaaaa ttcaaagaaa agagactttt
    42181 gatatccttt gagatatttc cgcccaaacc tgattaccct attgacacaa tttataaaac
    42241 aattgatgaa ttgcattccc tggctccaga ttttatgagt gttacatatg gagccggcgg
    42301 gagcacaaag gacaggacgg ttgaaatatc atcatatatt caaaacaagt acaatttaac
    42361 atccaatgct catgttacct gtatatcctc aagcaggaat gaaattgaga atatttccaa
    42421 ttcattaaag gaaaataaca ttaaaaatgt aatggccttg agaggagatt atccaaagga
    42481 ttatataccc ggttctcttc cctctgacag atataaaaat tctgtggacc tgatagaata
    42541 ttttaaaaaa aatactgact tttgcattgg agctgcctgc tatcccgaaa aacatcctga
    42601 tgcaagagat ttaaatgcgg acattgattt tctaaaaagg aaacaggatg caggtgctga
    42661 ctacctggtt acacaattat tttttgataa tggaatattt tatgattttt atgacaaaac
    42721 acaaaagaaa ggcatctcac tgcctatatc tgcaggaatt atgcctatac tcaacaaaag
    42781 acaaattaac acaatagtaa gactcacggg taattccata cccaagagat ttatgagatt
    42841 gctggaaaaa tatgaaaata atcccgaagc attgaatgaa gcaggtatgt cttatgccat
    42901 aagccagata attgatcttt tatcctgggg agttgccgga attcatgttt ataccatgaa
    42961 caaatcttcc accgcaggaa aaattttaga aagtatttct gcaatcaggg gtgtattaac
    43021 taatgagcgt aaaatatctt gacaatgatt taataaaaat tgataaaaca gaagctttaa
    43081 ggtatttaag atataaaaat gaagatgtag acgagaacac cggcaggctg cttgatgaat
    43141 ccgctgctga attaaaaaaa atctgccggc ttaaatatgt cttcaggatt ttcaatcttg
    43201 aaaaggaaga taataacata agctttgaga atcatataaa aattaaatct gatgatttga
    43261 aaggactttt taagcactgt gataagtccg ccgtcatggc agccaccctg ggatttgaaa
    43321 ccgagaaaag aatcagatat tacagtatgg ctgatttatc aaaagccctt gtatttgatg
    43381 ccctggcagc agcctgcatt gagtctttgt gtgatgcctg tgaagctgaa ataaaagaaa
    43441 ttgctgaaaa agaaggatgc accattactt tcagatacag ccccggatac ggtgatgtgc
    43501 ctatttccca tcagcgtgaa attctatcgg cactggatgc tcaaaagctt ataggcctca
    43561 cagtctctga ttcttcaata ctgataccga gaaagtccgt gactgcattt attggatttg
    43621 ataaaagcag aaatttttat aaaaactctt gtttaaactg cagttttttc caaagctgtg
    43681 atttttctaa agaaggtgat aactgtgtta aataaattaa aggaaaaatt tttatacttt
    43741 gacggtgcaa tgggaaccat gcttcaaaac agcgggttaa aagttggaga attaccggaa
    43801 gagttaagta ttacaaagcc tgaaattatt tataatatcc ataaggatta tttaaatgca
    43861 ggctcaaata ttgtgactac aaatacattc ggggcaaaca ggctcaagct gaaaggctcc
    43921 ccttacaggg tcgaggaaat catagctgca tcagtaaata cagcaaaaaa atccatagca
    43981 ggtatggaag aaaaatatgt tgccttggat ataggtccca taggaacgct tctatcccct
    44041 ttaggtacac ttggttttga tgaagcctat gaaatattca aagaacaggt aatcatcggg
    44101 acaaaagaag gagccgattt gattcttata gaaacaatga ctgatttata cgaattaaag
    44161 gctgcaattt tagccgccag agaaaactcg gatctccccg tttttgcaac aatgtccttt
    44221 caggataaca gccgcacctt tacgggaact gatgtgaaaa cattcgtatt taccgctgaa
    44281 ggacttggcg ttgatgtttt gggagtaaac tgctccttag gtccgaaaca gctgcagcct
    44341 attgttgatg aattggtaga attcagttcc ataccgatta tggttcaggc aaatgcaggc
    44401 ctcccgaaat atataaacaa tgcttcctat tatgatatca gcccgttgga atacagcagg
    44461 gaaataaata taatggcaaa aaaaggcgtg gcgatatttg gaggatgctg cggcactact
    44521 cccgaatata tcaaaagcat cattaattcg cttaataaga tgaagccagt tgaaatttca
    44581 aaaaaatact ttacaactgc aacttcatct tcaaaaacag tatttttagg ccaggaagta
    44641 aaaataatcg gtgaaagaat aaaccccacc ggcaaaaagc gtttaaaaga agccctaaaa
    44701 gaaaataatc ttgattatat tataagagaa gccataatac agcaggaaac aggctctgat
    44761 atacttgatg taaatgtggg cttccccggc gttgatgaaa aacaaatgat gaaaaaagta
    44821 atatcagaaa tacaaagcgt tgtttccctg cccctccaga ttgacagtgc caattatgat
    44881 gttattgaag ctgcagcaag aatgtataac ggcaaaccct taatcaactc tgttaacggc
    44941 aaaaaagaaa gcatggaaaa actatttcct attgtgaaaa aatatggtgc ttcgattgta
    45001 gccttgactc tggatgagga aggtattcct tcaaccgcag aaaaaagact ggaaattgca
    45061 tctaacattg tggaaaaagc aaaagaatac ggcatagctg aagaaagcat actgatagac
    45121 ccccttgttt tgactgcttc cgcccagcag acagccgtta tggaaaccct taaagcaatt
    45181 ccgttaatca aaaataaata caaagtgaaa atcattttgg gaaccagcaa tgtatccttc
    45241 ggattaccct gcaggaatct tttaaacagc acttatcttg ccattgcttt aggctatggt
    45301 ctggacgctc ctattacgga ttcaaccaac caggtattaa tggatgttgt aagagcatat
    45361 aaagtattgg ctgcccagga taaggacagc agggactaca tagaagctta cggcaatata
    45421 agtcctgaaa gcaatgtaat tactgatgat tacgatttaa aagaaattat tgtcaagggt
    45481 ttgaaggatg aagccaggaa ggtttcatta gaactgctta aaaagcataa acctcttgaa
    45541 atagtaaatg aattcatgat acctgccctg gatgttgtag gtgaaaagta ccaaaagcag
    45601 gaaatatttc tgccccagct tataaggagt gcagaaactt ccaaggaagt atttgataca
    45661 ataaaggaaa agtctacaaa aaccggagaa attgaaggta aaaaaatact cctggcaaca
    45721 gtcaaaggag atatccacga tataggcaaa aatatagtca agcttatact tgaaaattac
    45781 ggatataaaa tttttgactt gggcaaggat gtggatgctg aaaggatagt tgaaactgtt
    45841 ggcaaggaaa aaattgagct tgttggtctg agcgccttaa tgactacaac tgccaaaaac
    45901 atggaagata ccatcaaatt gctgaaaagt aaattcccag gtataaagat tatggttgga
    45961 ggtgcagtat taactgctga ttatgcttct gaaattggtg cagactacta ttccaaggat
    46021 gcaagaggta cggttgatat tgctaagaat gcatttaata gttaaatgac tgccctaaag
    46081 ggcagtcatt tacgattttt caaggacggg ctattccatc acagaggttg attttccgtc
    46141 cttcatatcc ccccgaaatc tatactattc agactcgtag actaatttat tttcattatt
    46201 ctgagtcagg aatatctttt ctatttcatc atccatcaac ccttcaatgt cattgcattc
    46261 aattttagca catacactgc aatttgagct taatatccgg ggtaccggca taagctcgca
    46321 atgtatattc tttcgtttgc acttcctgtc gaattttatt gcgcctgaat gggtgaaaaa
    46381 caaaactgtg tattccatct tactttctag cttctatcag aaatgtgtcg ccagattctg
    46441 ttattgttgc agtatagcct gcacttttta agaatctttc cacattaccc ctggcagtag
    46501 aattgtctac tactatatcc aatccttcag gattctctcc caatgctttt ttagtcatca
    46561 gcacaggctg aggacagctc atgccgcatg tatctatttt tttcatgatt acctcctatt
    46621 tagcatctgc ttttgacaag ccttcacttg cggttttctt aactaggtct tctgaattta
    46681 agtatgaaac aaggcctaaa aatgcaaagc atatgagtcc tgcaaccttt ccgtttggag
    46741 ttactcctgc agcgcttgaa gccagtccga aattatgggc aaaagcagct ccaaccaaca
    46801 tacccattac agtaataaca gagtctgtat tgccttcacc tgacagaatt aattgtctta
    46861 gagggcatcc tcccaatagc acagagcccc agcctacaac tgccatgccc aggaaatccc
    46921 acaatccgtc agtgtgagca ataggctgtt ctgcaaaccc aagtttgaaa aatccgaata
    46981 ccatgttccc ggcaaaagca gctacaagta ttgcaataaa tcctgacaag agatacatat
    47041 ctttaaaaag tatcaagtca cgggttccgc caaccgtaca cattcttgtt ttttgggcta
    47101 atgctcccac tatcaaacct gctgccaagg atacggccaa aggtgcatgc attgaaccgg
    47161 gaccttttgc cgagaagaac aagaaggcag gtgctacaac aagaagaact aataatccaa
    47221 cattcactgc cggaaaaata taaccttcta ttttagtgat gttgtagctt ctttttaaat
    47281 taaatccttt attaaggaat acaactccaa caaaaattcc tgccacgaag cctgcaagac
    47341 ccaataaagc atttaagtct ccccctgcaa gcctcaatac cattctaaga ggacatccca
    47401 ggaatattaa tgctccaatc atcacaacta ttccaagcat gaatctggtg aaaggagatg
    47461 aacctccctt agtttgaaat tctttgttta ttaaagccat tgagaatgct cccaatacaa
    47521 gaccaataat ttcaggtctt atatactgaa caacttctgc cctatgtaat ccgattcccc
    47581 ctgcaatatc tcttataaaa caagctatgc aaaagcccat gtttgcagga ttccccaatt
    47641 ttacaagtaa tactgctatt attcccacta gtccgcctgc aattataatt cctcttttat
    47701 ccttcataaa aacctcccgc tttatttatt ttgtaacaaa taaaattata ttatggaaaa
    47761 ttatttaaaa caataggtat aaattttttc aatacttgga ccttcaacta tcaatttttc
    47821 ttattttgtg tcgaaaatca ttgaattata aacaatctgt tggctatgcc agactaatac
    47881 caaaagttat atttcgacat aatatgaaaa tctcaattga ataaatatat gacatatgtt
    47941 aaaataagat aatccttgaa aattaaggat taaccagctc tttacaggag gcgataccat
    48001 gagatatgta tatttggaca atggtgccac atctttccca aaagctcccg gtgtagcgga
    48061 aagtatttca gattatattt taaatatagg aacaaatgtt aatcgaggag cttacagctc
    48121 atctttcaaa gctgaaaaca ctgtatatga aaccagggaa cttttatgtg aattgtttca
    48181 ttttgataag cctgaaaatg tagttttcac taaaaacata actgaaagtt tgaatgttct
    48241 gataaagtcc ttgttgaagg aaaatgacca tgttgtggta tcatctatgg aacacaatgc
    48301 ggttatgcgg cctttaaatt cacttaaagg ggttgaatac accagggttt tatgcgatga
    48361 aacaggtaat ttgcctgttg aagatgtaga aaaagcaatt aagaaaaata ccaaagccgt
    48421 tataatgacc catgcttcaa atgtgtgcgg aacagtttta gatttggaaa aagtcggaca
    48481 aatatgcggt aagcataatt tgttttttat aatagacagt gcgcagacag cgggattttt
    48541 agatattgat atggatggat taaatgctga tgccattgga tttacaggcc ataaaagttt
    48601 gctgggacct cagggaatag gcggatttat tgttaaggac agaatgaaca atgaaatgac
    48661 tacttttata gaaggcggca caggcagcct gtctgatacg gaaatacagc ctggctacat
    48721 gccagataaa tttgaatcgg gaaccttaaa cataccgggt gtttacggat tgaatgcgtc
    48781 attgaaatat cttttgaaaa aaggtgttaa aaatatcaga gaaaaggaga tttccctcct
    48841 cagcagattt ttagacggtg ttcttaacat tgaaaatatt aagcttgtgg gtaaaaccac
    48901 aacccttgac aggacaggca tactgtcaat tgatttcatc aataacgaca atggccttgt
    48961 ttcacatgag ctcagcaaag aatacgggat tatgaccaga agcggcatgc actgcgcacc
    49021 atctgcccat aaaacattgg gcaccttccc caagggaacg gtacgttttt ctgtaagtca
    49081 ttttaacact ctggaagaaa ttgattatgc catagattgt ataaataaga tagcaaaaat
    49141 tgataattgt tgataaggtg acaatactcc gtccccttgt catatggagg cagtatatgg
    49201 aatttaacca gctggagagt tttgtaaggg ttgttaagca taaaagtttc tcattggccg
    49261 caaaagaact ttacacaact cagcctacaa taagcaataa tattaaaaac ttggaaaaag
    49321 aactgaatac tactcttttg gacagaacca gcaaaacaat aaacttgact gattccggca
    49381 agctctttta taaatttgcc gtggaaatga ttaacacaag agacaaagct aagttcacaa
    49441 tatcagaaca tgacaacaag gtagagggtg taatagaaat atgcgccagc tccatacctg
    49501 aagaatatgt gctgccttat ataattaaag attttttaaa aatatacccg aaggtttcgt
    49561 tttctgtaac ccataaaaat tcaaaaaatg taatagatga tattttagag ggaaggctaa
    49621 actacgggat tgtgggtgca aagtattatt cagagttact tgaatacata gatttttatg
    49681 aagatgagct gattttgtgc ctgcctgcag gcagcaaatg cctgtggtct gaaaatgagc
    49741 acatggatat taaacaattg ttttcggaaa aattcatctt cagaaaacca ggctcaggaa
    49801 ccagaagatt aattgaacag aggttttctg aaaaaggcat cagcctggac gatttaaata
    49861 ttatttctta cattgacagc aatgaaatga ttaaaaaaat gattgagctg ggattaggca
    49921 taagctttat ttcaaaaata gctgttaaaa atgaaattga tttaaaactg gttaaaacaa
    49981 tgcaaataaa agacctggac ttaaaacgaa gtttttattt tgtccgcaac aagaacagga
    50041 ccctgtcccc cttagtggaa gcttttaaaa attttttaat atcctggaaa tattaaatga
    50101 ggaagtgtaa atatgccgtc aaatgatata aagttaactc aaatgaccaa aagctccggc
    50161 tgagcagcaa aaataggacc tgatgtcctg gcgcaagttt tgtgccactt aaactatgat
    50221 tctgatgaaa atctgcttgt tggaatggat acatctgatg atgcatcagt ttatagaatt
    50281 aacgataacc tggcattggt acagacactt gattttttta cacctgtagt tgacgacccc
    50341 tatgtgtacg gtcaaatagc agctgcaaat tcattgagtg atgtatatgc catgggcgga
    50401 gagcccaagc tggctctgaa tattacatgt ttcccggctg acctgcccaa ggatattgta
    50461 aaaagtattc taaaaggcgg ccatgataag gttaaggaag ccggggccat ggttatcggc
    50521 ggtcataccg ttgaagacac ggagccgaaa ttcggcttgt ccgtaacagg tttcatacat
    50581 cccaaggatg tggtaaccaa cagcaatgca aagcctggag atgttttaat tcttaccaag
    50641 cccttgggac ttggaatttt aaatacaggc ataaaggcag gaatagtaag cgaggaaggt
    50701 tataaaacgg ctgtccgtac tatgaccgag ttaaacaagt tttccaagga tgcaatgatg
    50761 aaggttggtg taaactcctg tactgacata accggattcg gtcttcttgg tcatgctttt
    50821 gaaatggctg aaggaagcga tgttacaata aagtttaatg caatgtcaat tccaattatt
    50881 gaggaagcct tatctttggc gagaatggga ataatacctg caggagctta cagaaattta
    50941 ggatacataa aaaatcaagt taaaatttct gataaggttc cacaggaagt aacagactgt
    51001 atgactgacc cccagacttc cggcgggctc ttgatatccg ttgaaaaaaa taaagctgaa
    51061 atgcttctta atgaactaaa aaacagcata accccctatg cgcagatagg tgaagtttta
    51121 gaaaagcagg aattcaggat aatcgtagaa taggaatttc tgtaagcata tatgaaaaaa
    51181 atccgtagaa agttctgcgg atttttgtgt tgattataat ttatactagt ctattatttc
    51241 tattatttgg tttatatcat tatcctgctg attattataa acaaagctgt catcttccca
    51301 cttcccctcc tcaaagcttc cgtctatata gtataaaacc cctgagcctt cttttttccc
    51361 gttcttaaat tcacccacgt acttatttcc attagcaaaa gtatatgttc ccaagccttc
    51421 cttcatgccg ttaacatatt ctccttcgta tttatcaccg tttgcataaa cagttactcc
    51481 atggccattt ataaaatcat ccttgaattc gccttcataa acatatctgt tgggcacggt
    51541 aaactttccg tttccatgct gaacattttc tttaaattct ccctcgtata catccccgtt
    51601 ggaatattta tatatgccaa atccgtccat cattccgttt tttatttggc cttcatatat
    51661 gtacatattg ggcgaagtta ttttaccatg tccctgcatt acattttcct tgaattcgcc
    51721 ttcatataca tcaccgtttg gatatgtgta caagccatag ccgtccatca ttccattttt
    51781 catttgaccc tcataaatat caccgtttgg atatactatt ttaacattac cctcacgaat
    51841 tgcttcctct gcttctgctt ctcctgaatc attgccgctg gattcttccc tgtcattgtt
    51901 tacatcacta ttgctttcat caggattaat cccgtcagaa ttattttcgc cggttttatt
    51961 taaatcttga ttattttcat cgttgtctgt attctcaata ctattgtttt catcaatgct
    52021 gcctgtattt tcgtttccgt tataactaag ctccagattc tcattattat cttcatatct
    52081 ggaacaagaa gatattatca acaaaatact cactaaaata aatgcaacaa tctttttcat
    52141 gtcagcctcc aatgccagcc ccttatattt atgtttatta ttgccgtcaa aagtaaaaaa
    52201 tagtaatggt attttctttc tcacctgtaa aaacatgtaa gccaggcagt tttacgcaaa
    52261 gaaaaaatca ggggcttccc tgattttttc tgttctatat acaatttagc tatcaacctt
    52321 tacttgttgt ttctgtcttt ctcaatgcta acagagtaag aaacatatta ataatgccaa
    52381 gaattgtaaa tgttatcagt atcatattgt ttgatcctgt aagatcatat gtaaaattga
    52441 taacaggtgt tccaagggca atgcctgctc caactaaagc agaagcaatt cctatgatat
    52501 cactcaggta ttcatctccc gaaattgcgg atgtaaacaa gggcagtaat actgaaagag
    52561 cacttaatga gataccgaat agtgcggcaa aaatccacgc aattatttcg ctgttgacta
    52621 taagaagaag cacagcacat attacgaagg acacaagaca tataaaaata gcagctttaa
    52681 tgccgattct gtcataaaca gctcccacca gaatctttga caatgccatt atgaacataa
    52741 ctccccctaa aattttggaa gcaaatattt catcaaatcc cctgtccatc agatgggaag
    52801 gaagaacatt aagccatgga gccattaaaa accccatcaa aaaaataaca gtaagaccta
    52861 aaataactct atgctcattc agcagctctc gtgttgtctt ttttttaaaa tttgaaactt
    52921 ctgtatgatt tgtttcaata tgctcgacag gattttcttt tatcgtcagg gcaattggaa
    52981 taatcagaat aaaaagaatg attgaagtca gcatatatga ctgtttaaaa ccgtaaaccg
    53041 atataatgta acccactaca gggctcatca cggaccctcc gaatccgctg ccggcagaaa
    53101 tcaagcctag aatagttcct ctgttttgta taaaccagct gtttataagc agtgaaaata
    53161 ctatcatatt tgtataagcg tgtcctattc caaacaaaat tgcacccgtg taaaactgta
    53221 taagtttagt tgatctgaag taaacaaaat aagctgaagc acaaaaaaaa gttcccataa
    53281 taaccagctt cttaatgccc aaacgcttgt acagcttaac aaaaacaaga tttgcaaata
    53341 tgctcagcat ggatataatg ctgaatatca atgaaaactc tgctctgcta aaaccataat
    53401 aacctgttac cggaactaca taaaggctgc ttgtattatt ggcatatcca atatagaaaa
    53461 atgcaataat aaagcacata agagctacga cataaccata gccaaatttt ttattcataa
    53521 aactccttgt gaaattattc tgtcttttgc attgctataa tttatcttaa tataaactta
    53581 gaattcaatg gactgattgt ttatatccac caggtatttt ctgcaagagc cttggaatct
    53641 gtttattaca agttgctcta tatccctcat agcttcaaat aaggaagatc cttcctcata
    53701 atcaaaccct acaagctgaa aaaatacatc actgctgtca agagtaagtt ttcccaattc
    53761 gctttgccgc caaagccatt tatatgtttg aactatattg ccgcttgtga atatcagctc
    53821 gttttcttcc acatcctcat aggtttccga gccgaaaggc agaaaaatat ccccttttct
    53881 gctgtacctg acctccaggt cctcatggat atctattaaa tcatggcctc ctaaggatat
    53941 gccctgttct atggataccg cattgcataa gtccaccagt gcatttataa caggcagctg
    54001 tcctcctttt actatccttt tgaacattgc ctctacagat ggaaggtatt tgtttgggtt
    54061 gatgcctgat ttcatcacaa cttccctgta aagtcttaca ttgttcaaat tccttatttc
    54121 atcagccttt acttcctgtc tcattttatc ttctgctctt ctaagtcttg cttcgtcttc
    54181 aggccttgta gaggaatttt ttatattgct gcctatcagt ataccaaatt taatatcagg
    54241 atttaattca aacacagact tgtcaattgt atatttcatt tttttctcct tacagttctt
    54301 tgcaatctgg tgattattgc acaactatat tttttatatt cttttccttt tggatttttt
    54361 ctatatgttc tgattccagg agccacgggt tattgatata tttttcaaaa agcttgaatg
    54421 acctggcata atatacaccg tcacatattc tctcatccat tgtgtaacct atttcacagc
    54481 attttttaat cactacctgg tcattttcca ctaccggaag ctttactgtt tttccaaggg
    54541 cagcaaatag tcccgtagtt ccaaaatcat agagatgatg atagatatac tgtatgtcta
    54601 ttgactttaa gtaggtaaaa aacatagtag tgtgaaaggg gcttgcatca attacattct
    54661 taggaagtaa attgagcata tcagctgctt ttataattct gaccaagatt ttcttcacaa
    54721 atccgggcat tgacattact ctttcggcca gcttgtcggt attattatct tcacttgagg
    54781 atactgccct gtctatgtct ttatctataa tttcggcaat ttcgaaaatg ttttctttgc
    54841 ctgtaaattc gaattttact gttgtttctt ctccatcatc tctgaatgat cgttttaccg
    54901 ccattgatag ataaatttta tttctctgat aaatttgact gctcatcaca aatctgttaa
    54961 gcattggtct ttctgcaata accctaacat aggcggctat gaaaacatgc atatatgaaa
    55021 aattataacc tgcctgcttt ttctctaaaa tatatttatc tatgttttcg gccggtatga
    55081 cctgttttga aaaatcttgc gaatcgcttc ttttttccat gacatagggt ataatcttca
    55141 caaatggttt caaatccttt aataaatagc cgtcacttct tttcataaat cctccaaaat
    55201 ctacatgatt atgtaattca gtccacctct tataaaacac agaggtatct ccgattttct
    55261 ctcaggaacg caacttaatc gaataataac atatttttac tatggtatca aatatattat
    55321 ttatttttaa tatttttctt gcatactgat ttcccataaa catatgtttc caaaaccttg
    55381 gtgtttatta cagtttactt ggttctaaca tattttacaa aacaattgtc tataattgtc
    55441 tttactttct ttatatcaca taagtattcc acgtaagact ttaacattct ctctgccatt
    55501 ttatatttat aaacactcat tacttccata ttaaactttc tgattattcg ttttaaaata
    55561 tcttcaaaag tcataggctt agttatttct tcatatacac tttcagccat gccgccgtaa
    55621 tatttaatat tatcctctat caatgcctct atatcataca atattccttt atgagtgatt
    55681 atataagtat ttgagtcaag ttgatgcaag gctttcttac tttccaaatc atcctttaac
    55741 acatatgcat aggggatttt tattcccctc attgcttcat gtgtaatcaa tgcatccgcc
    55801 agataacaaa catcatcagg agttgtaatg catatttggt cagaagagtg gccgaacaca
    55861 tgaaatagtt taaaaacaac atcatctaga attatgttgt cttgtttttc atcaataata
    55921 atatctgttt cacagataga ataattataa taactcttaa ttagtgacat gggctggtcg
    55981 taaaaaagtt ttaggctggc aaatgatgta caaataaatg cttcatcttt ggtcatggca
    56041 atcttacagt cgtatttttt cttaaaataa gcgttgtttc ctatgtggtc aggatgagca
    56101 tggctgcaca atatggtttt tattcttaaa ttgtaagctt caagaacttc ttccaatccg
    56161 tccaattctt tgtgatagcc ggaatccatc agtataatat cttgcttgtt aagcttataa
    56221 aaaggtatgt aaatagtacc tgtatctata caataggtgt ttccttttac tttagcaatt
    56281 tccattctac tctccttgaa attcatcagc ttgtaacata ttttactata attcaataaa
    56341 gtttgtaaat caatagaaaa ttcaaatggc gcatatcctg cgccattatt atttgcttat
    56401 tgtttttatg tatttattta tattctttaa ttaatttttc aaatacaggc aatgctttct
    56461 cgcatttttc agtatcaacg caatatgaca gtctcaagtg tgtgctggtt ccaaagcccg
    56521 ctccgggtac aaccaggaca tcatgtgctt ttgctttttc agaaaattct tgggaatttc
    56581 cgtttggagc ctctataaat aagtagaatg ctccatcggg cttggcaaca ttgtagccca
    56641 tttctgtcaa gccattatag aggatgtctc tgtttttctt ataaacttcc aggtccggct
    56701 caacatcaat gcacttttca ataacaagct ggaaaagcgt aggagcacat acatatccga
    56761 gagctcttgc agctcctccc acagcagcta ttagagtttc attgtcctca catttgtcat
    56821 ttactaaaat gtagccaata cgttcaccgg gcattgataa ggattttgac catgaatagc
    56881 atataattgt attgtcataa taattaggaa taaaaggtac aggctcatca ttgaaaacta
    56941 attccctgta aggttcatca gaaatcaaat agattgtttg acctgtctcc tcctgcattt
    57001 tcttgagata atctgacaat tcctttattt cttcctttga atatactact cctgaaggat
    57061 tgttgggtgt attaataagc agtgctttgg tatttt
//
LOCUS       DAOPUT010000003        44519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_778_length_44519_cov_19.228859, whole genome shotgun sequence.
ACCESSION   DAOPUT010000003 DAOPUT010000000
VERSION     DAOPUT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 44519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44519
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_778_length_44519_cov_19.228859"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..633
                     /locus_tag="PKK75_00760"
     CDS             118..633
                     /locus_tag="PKK75_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNZ82153.1"
                     /translation="MINEKVAKLMNEQINKELYSAYLYLDMANYYIDKGLDGFANWFK
                     VQAQEERDHAMLFMQYMQNNQEKVTLEAIQAPDVKFDSFASPLNEALKHEQYVTASIN
                     NIYAAAYEIKDFRAMQFLDWFIMEQGEEEKNADDLITKYNLFGTDSKGLYMLNSEMGT
                     RVYSPPSLVLD"
     gene            complement(689..1387)
                     /locus_tag="PKK75_00765"
     CDS             complement(689..1387)
                     /locus_tag="PKK75_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ82154.1"
                     /translation="MIYRQNQQLCPNINSMPYEVRPGDTLNSIATRFGTNLVGLIELN
                     PTLRLRSLTEGMMICVPKVPERPPCINGAYYAIREGDTFYNLAQRYGIPLNLLLEANP
                     EANPYDLKVGQEICIPNVPAGCPFGTVVTIQEGTRLSDILISYNLSLNELRDTNPNFN
                     PNIIVPGEQLCIPPEAFMQCPQGTRGEYVIKAGDSLSTVAEANGLTTSGLLIANPHLR
                     PANFLIIGTRVCIP"
     gene            complement(1448..1894)
                     /gene="rnhA"
                     /locus_tag="PKK75_00770"
     CDS             complement(1448..1894)
                     /gene="rnhA"
                     /locus_tag="PKK75_00770"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNZ82155.1"
                     /translation="MKKVSIYSDGACSGNPGPGGYGVILEYKGHEAELSGGEENTTNN
                     QMELMGVIVGLEALKEPCDVTIITDSKYVTDSFNKGWIDIWQEKGWKKSDGKKVLNKE
                     LWRRLLKAADGHKVKFVHVPGHQGHSYNERCDKLAVQERKKFEQSY"
     gene            complement(1900..3072)
                     /locus_tag="PKK75_00775"
     CDS             complement(1900..3072)
                     /locus_tag="PKK75_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HNZ82156.1"
                     /translation="MYKVALFCNIPQVTRNLFPQDKYSLIKEVTSEADGIILRSLDIN
                     GMKFSKQLKAIARAGAGVNNIPVERCSEEGIVVFNAPGANANAVCELTFLGLLMSSRR
                     VIDSIDWTKKLESNIVETVEKGKSQFVGRELKGKTLGVVGLGDVGHLVANLGVKLGME
                     VVGYDPYISVKNALALSWDVHYTVDINDLYRKSDYITIHVPYNNETKNKINGDSIKSM
                     KDGVIILNFARHGIINEDDLVEALENGKVDYHVSDLPNERLLRTKNTLCIPHLGASTI
                     EAEENCAEMAATQITEYLENGNITNSVNFPDCSMDRYPGTDRIIVLNKNIPNMIGQMT
                     TALASHNVNIIGMVNKSKGDYACNIIDVIGEFNEDKLKELEDQYGILRVMLIKNKV"
     gene            complement(3359..3499)
                     /locus_tag="PKK75_00780"
     CDS             complement(3359..3499)
                     /locus_tag="PKK75_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82157.1"
                     /translation="MSDEVKAANFGCYDRGNDWIEWLIILGIIFFLLGGDSFFGRGGC
                     CR"
     gene            complement(3591..4751)
                     /gene="thiI"
                     /locus_tag="PKK75_00785"
     CDS             complement(3591..4751)
                     /gene="thiI"
                     /locus_tag="PKK75_00785"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNZ82158.1"
                     /translation="MYNIAAVSFGELYLKGKNKGSFEQKLIDQIKYALKEFKDVSVYK
                     DSGKVFIESKNEGDMDKIVEKARKVFGISIISPCVKTTKDVDTIIEKTIELFGYLLSR
                     QDIKTFKINTKRSDKDFPFKSMDFSALIGGKILENFSSVRVDVHKPDVEVFIDIRKSC
                     YISSEKIKTLGGLPVGSSGKALLLLSGGIDSPVAGYMIAKRGVEISALYFHTYPFTSE
                     RANEKVVKLKEILEEYCGKIKLYSVNILEIHKAIREFCKEEETTILARRFMMRIAEKI
                     AQENKIDMLVTGESLGQVASQTMSSMAVIENAVDMPVLKPLVGLDKSEIIERAREVGT
                     YETSILPYDDCCSVFAPKHPLTKPKLENIKKSESKLDVAELIEKVYATIEIL"
     gene            complement(4754..5896)
                     /locus_tag="PKK75_00790"
     CDS             complement(4754..5896)
                     /locus_tag="PKK75_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNZ82159.1"
                     /translation="MIVYLDNSATTKIRDEVMKEIADANENLYGNPSSLHRMGLSAEK
                     KINEARKFAARLIGAKPQEIYFTGGGTESNNIAIFGHLSNLNKQNNIITTKIEHPSVY
                     NVFKQFEDKCQVKYLGTDEKGRINIQELGDMVDENTLLISIMHVNNEIGAVQELEEIA
                     AVIKRKNKNARLHVDAVQSYGKIKIDVNKLSLDTLSFSSHKIHGPKGVGGLYIRSPLK
                     LGPLVFGGGQERGIRPGTENTTGIMGFGKACELLYDSFKEETIKLYSLKKIYVERLKE
                     IEDIKINSPEEGAPHIISVSFKNVPGEVLVHYLEDRYIYVSTGSACSSKSRDNRILDA
                     INLGREYREGTIRISLGHFNSLEEVEYVIDSIKQSVKDIRSITRRG"
     gene            complement(6271..7083)
                     /locus_tag="PKK75_00795"
     CDS             complement(6271..7083)
                     /locus_tag="PKK75_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNZ82160.1"
                     /translation="MLNIVSDKNVLNNKIITKEETEKLLNQTKIYEVIRVIDRKAIFL
                     KEHFDRMNESIHLSGIKGSLCYEDFKSSAELLIKENPFKNLNIRVSYFYDNKPFALFY
                     FIESYYPAQEEFNEGVDTVTVKMERANPNVKSYQKEFKDKVTRIIEEQNAFEAILVNQ
                     DDTVCEGSKSNIFFVKGDKLVTSPDYAVLLGVTRSRVISVCEENLIEVEKRIIKFSEL
                     ESFDGAFITGTSNDVLPIRSIDNIKFNSSGNDLVKKVSGLYMGEVRKEIEKQ"
     gene            complement(7125..9425)
                     /locus_tag="PKK75_00800"
     CDS             complement(7125..9425)
                     /locus_tag="PKK75_00800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNZ82161.1"
                     /translation="MDNIKLSVRELVEFVYKSGDINVKNLSTDRALDGIKAHKILQSQ
                     MGENYLKEFYLKSEFILNDIVFSIEGRADGIIIEEDEITIDEIKSTYTDLSYIDDEYK
                     VAHMAQAKCYAYMYGFINRLQKLNVQLRYYNLDSNETKIIKQTFTTEELETFFYDLLN
                     TYIDWAETIIKLQKERNKTIKETIFPFDDYRKGQRDFAVAVFLSIRSGKKLFAQAPTG
                     VGKTVSVLFPAVKSLYEKNAKIFYLTAKSSTKNIAFNTVKMMYEKGLRLRTTIITAKD
                     KICFMDETKCEPEYCPYAKGYYDKLNIPLRETIKNDCLYDRDFIEELGKKHELCPFEL
                     SLDLAYMSDIVICDYNYYFDPRVALQREDVFNNDKDILLIDEAHNLEDRARSMYSPEI
                     IKEEFYEVYKMMKSEKSLSKELFNINKKFIDIKKIVEEAQILNEPPEELTNALRKFAA
                     RAEKYFNDKKNEKVPDELTDLYFKTTFFIKISEVADENFCYYAEKANNKTMVKLFLID
                     PSSILKEIEKNAQSSVFFSATLTPLKYFRYILGGDEDDYILKLNSPFERENLNLMISS
                     DISMKYTMRDSNIEKACEYINALITEKKGNYMVFFPSYYFMSKVYSIYESLYDSTGIT
                     VQNQGLNEEEQRDILDRFENERGVVLFTVVGGIFSEGVDLPLEKLIGAVIIGTGIPQV
                     SFERNIIKAFFDEKKNSGYDFAYKYPGFNKILQSAGRVIRTEEDKGAVLLIDSRLCQP
                     SYLKLFPDHWKHFKKVSSAEDVKQFL"
     gene            complement(9497..9883)
                     /locus_tag="PKK75_00805"
     CDS             complement(9497..9883)
                     /locus_tag="PKK75_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ82162.1"
                     /translation="MHYVVQRKVNSDIKCGKKIIEEILRNIKGILDPDTFFNTKLILY
                     ELMINSVIHGNCGDLNKMININVYINKSRVIIEVSDEGSGICYDLKDCGEYDYLESGR
                     GLMLVEGLSDKLNIDGNTVTCVQYLK"
     gene            complement(9982..10575)
                     /gene="yihA"
                     /locus_tag="PKK75_00810"
     CDS             complement(9982..10575)
                     /gene="yihA"
                     /locus_tag="PKK75_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNZ82163.1"
                     /translation="MIIKKAEMIISAVKKQQYPDTTVPEIAFAGKSNVGKSSMINALL
                     NRRSLARTSSQPGKTQTLNFYNINDEFNFVDLPGYGYAKVSKNEQLKWAAMIDSYLHG
                     RQQLKEVILLVDIRHEPGKNDLQMYEWIKSVGFKGYVIAAKADKLSRSQQLKNISLIK
                     KTMQINDSSLILPFSSTSKLGAEEIWILIENILNLQI"
     gene            complement(10857..13202)
                     /gene="lon"
                     /locus_tag="PKK75_00815"
     CDS             complement(10857..13202)
                     /gene="lon"
                     /locus_tag="PKK75_00815"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNZ82164.1"
                     /translation="MINNYIIERRNLPLIPIRGIGIFPNTVIHFDIGREKSINALEEA
                     MLEDSDIFLTVQKVADMESPKEDDFYEVGVICKIKQMIKMPGDNIRVLVEGINRAKIE
                     SITQNDPYFEVVLDEYIYDDSLPVDDNLEAMVRLTLNNFEEYALIYAKIAPDAVISLK
                     EIKNPDKLADVVASYIYLKTNQKQSLLEIFDPYKRLESINKLLAKEIKVLQIENEISE
                     LVKKQVSEFQKDYYLKEQMRAIQKELGEDEGTDYEVEEYLQKIAKAHMPKDVKEKAEK
                     EINRLLKISPSSPDAGVIRTYVDWLIDLPWDKKTKDNTDLSKARSILEEDHYGLEEVK
                     ERILEYLAVKHIKKDMKGPILCFVGPPGVGKTSIAKSIARAMDRKYVRISLGGVRDEA
                     EIRGHRRTYIGAIPGRIISSIAKVKENNPVFLLDEIDKLNGDFKGDPASALLEALDPE
                     QNSTFTDHYIEAPFSLQNVMFITTANTTATIPGPLLDRMEVIEISGYTDVEKRNIAEK
                     YLIKKQLSEHGLNEDQVNISTNAIDEIIGRYTRESGVRNLERNIGKLIRKAAVQVVEK
                     NKKSVSINNSNIKKYLGIPKYDYDTAYEEDQVGVVTGLAWTQVGGETLFVEVNTMKGD
                     GKLQLTGQLGDVMKESAMAALSYIKANAEKFGIDYQIFKETDIHVHVPEGAIPKDGPS
                     AGITMATAIISALTKKPVRKDVAMTGEITITGRVLPIGGLKEKLLAAKRAGIKKVVLC
                     SKNKADVSKVDPKIIRSMEIVYADKIDDVVKEAVQQPETEK"
     gene            complement(13398..14657)
                     /gene="clpX"
                     /locus_tag="PKK75_00820"
     CDS             complement(13398..14657)
                     /gene="clpX"
                     /locus_tag="PKK75_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNZ82165.1"
                     /translation="MGKVNEKHVKCSFCGKSQEQVRRLIAGPDVYICDECIELCHNIV
                     DEDVDIFEEYDFTELPKPKEIKEKLDEYVIQQDNAKKALAVAVYNHYKRINYKAKDEG
                     VDIQKSNILLLGPTGSGKTYLAQTLAKLLNVPFAIADATSLTEAGYVGEDVENILLKL
                     LQAADFDVEKAEKGIIYIDEIDKISRRSENPSITRDVSGEGVQQALLKILEGTVANVP
                     PQGGRKHPHQEFIQIDTTNILFILGGAFDGMEKIVQKRVGKKNIGFGADISGNTAMDT
                     DKLLKQVQSQDLLKYGMIPEFVGRVPVVVALEKLDRKALISILTEPKNALLKQYKELF
                     KMDNVELEIEKEALELIAEKALEINTGARGLRGILENIMNEVMYEIPSRSDIKKCVIT
                     KDTITNNSEPKLVLTKKPVSDKKENAS"
     gene            complement(14670..15254)
                     /gene="clpP"
                     /locus_tag="PKK75_00825"
     CDS             complement(14670..15254)
                     /gene="clpP"
                     /locus_tag="PKK75_00825"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNZ82166.1"
                     /translation="MALVPYVIEQTGRGERSYDIYSRLLKDRIIILSDEVNDVTASLV
                     VAQMLFLESEDPDKDIQFYINSPGGSITAGMAIYDTMQLVKPDVSTICVGMAASMGAF
                     LLLSGEKGKRFALPNSEIMIHQPLGGMSGQASDIKIHADRIIKIRDTLNKIISEKTGQ
                     PISKVEQDTDRDNFMTAEEAKIYGIIDDIIVKRK"
     gene            complement(15324..16703)
                     /gene="tig"
                     /locus_tag="PKK75_00830"
     CDS             complement(15324..16703)
                     /gene="tig"
                     /locus_tag="PKK75_00830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNZ82167.1"
                     /translation="MSSKVLKKENNTVTLEFTVSQDDFEKAVNKAYLKAKNNINVQGF
                     RKGKAPRHIIEKKYGKGIFYDDAMDIAVQEAYPAAVKELELDIIDSPKIDVEKFEEGE
                     DLIFKAEVEVMPDVELPEYKGIEVVKTEIKVTDEDVERELKSVQEKNARIVEAHDRPV
                     QKGDFLTIDYAGFVGDEQFEGGTAENHTLEIGSNSFIPGFEDQLIGKSKGEEVTVNVT
                     FPVEYHAENLKGKDAVFNVKIHEIKVKELPETDDEFAKDVSEFDTLEELKADTRSVLE
                     KKAADRVKISNDNNVVTKLVNDTKLEIPEVLIQREVDLLGRNYEQQFRQQGFNGKEYD
                     ELIENFVKQYKEGAREQAKFNVKAELVLEALIKKEKISVSEEEFKEEVNKIAESYNVE
                     EERLEAFKEGLMQSSKSYIEETLQKRKAIDMLVENAVFVDAPEKTETDAAVEAAESAG
                     AAEHIKDKE"
     gene            complement(16797..17357)
                     /locus_tag="PKK75_00835"
     CDS             complement(16797..17357)
                     /locus_tag="PKK75_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ82168.1"
                     /translation="MEKLVLSGTVQLETKRFLLRKFQMSDAHDIYSNWLSDEDSARYN
                     AWRVHSSIEVTKEYLSEWISLYKKNDYYHWAVADKVTDEVIGSVTVSNIKKRKKYCEI
                     GYTIAKKLWNKGIATEVLICTLNYLTQEAGFERICALHDIRNEASGKVMKKAGMQFVK
                     NKTQIYLNSNNIIMKCRLYEYKKSLL"
     gene            17601..18239
                     /locus_tag="PKK75_00840"
     CDS             17601..18239
                     /locus_tag="PKK75_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNZ82169.1"
                     /translation="MPQEIYNASGIKVFGKRIKSLIYTTDLAIIKNNNADGVIAVYPF
                     TPQIAINQAIIELSSTPVFVGVGGGTTTGQRSIDIALNAELTGAYGVVLNAPTKNELI
                     SEMKERLDIPIIITIVSEKEDFESRINAGVSIFNVSGGANTARIVRQIREKHPSFPII
                     ATGGPTPDTILETIQAGANTITYTPPSIGDIFSEMMEVYRHRIIDELDKKNK"
     gene            complement(18298..18741)
                     /locus_tag="PKK75_00845"
     CDS             complement(18298..18741)
                     /locus_tag="PKK75_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNZ82170.1"
                     /translation="MDILNLLERIEDIIEDASKFPLSNKVMVDKEEVLEVINEIRLKL
                     PDEINRASWVAKERQRILNEAQTEADELVIKVKEQQKHLIEESEITRQAKNYANELIQ
                     EAQQRANEMKTGAYNYSDEILSRLQDKIKEINNIIEDNRDVLKKM"
     gene            complement(18743..19222)
                     /gene="coaD"
                     /locus_tag="PKK75_00850"
     CDS             complement(18743..19222)
                     /gene="coaD"
                     /locus_tag="PKK75_00850"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNZ82171.1"
                     /translation="MRVLYTGSFDPITVGHLDIIKRISKKFDTVIVTVFNNQEKKYWF
                     PIEERCEMVRAAVEGLTNVEVDFTSGLVVDYCARKDISIIVRGLRAVSDYEYELSISS
                     INKHLSKELETFFLVATPQYSFLSSSMVKDVAEHGGRFEDLVPENVVQNIYRLLGRK"
     gene            complement(19227..19790)
                     /gene="rsmD"
                     /locus_tag="PKK75_00855"
     CDS             complement(19227..19790)
                     /gene="rsmD"
                     /locus_tag="PKK75_00855"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNZ82172.1"
                     /translation="MRIISGTNKGKRLYAPEGKEVRPTGDKIKEAVFNIIGSIDEDST
                     VLELFAGSGSMGIEFLARGAKHCTFVDISKKSLYYVKKNLDLCNFTGKAKIIAGDYEK
                     TIINLSNNKEKFDYIFADPPYGLTLGNKILNAVIENGILKYGGILVIESEKSEKVIDN
                     EALDVVEYKEKIYGRTKISIIRLLEGK"
     gene            complement(19874..21925)
                     /gene="recG"
                     /locus_tag="PKK75_00860"
     CDS             complement(19874..21925)
                     /gene="recG"
                     /locus_tag="PKK75_00860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNZ82173.1"
                     /translation="MLDADIQYLKGVGPARALKLQKLNIFTVEDLLNYFPLKYEDRRT
                     IKNINEVCEQGKYLLKVRIFEKPKYSRIKKGMTIIKAICKDDTGFITLTFFNQDFLKD
                     KLIAGEYYYIFGNIKTAYGKLEAVNPEIEPVDKDWKAGNIMPVYGLTYGLSNNEITKL
                     VKTALETYYDEIKNILPEEIIKKYKLTSKKIAVKALHFPSDNKKYSLAKRTIAFEKLL
                     IMQLGLFNIKNNLSNGVSHIEIRNTGLSEKLLKSIPYTLTNAQLKVISQIKEDLKSQK
                     TMNRLIQGDVGSGKTVVAVYAMLMAAASGYQSSMMAPTEILALQHYETIKEFIEKADL
                     DVKIEVLTGSVKPKDKEEILQKLKSGEIDIIVGTHALIEDNVEFNNIGLVVTDEQHRF
                     GVRQRARLSNKGKAPHILIMTATPIPRTLALMIYGDLDISLIDEMPPGRQKVITSIIS
                     GKEKEKAYDFIKKQADSGRQAYIVAPLVEESENLDLNSAVEIFKELKSSYFSSYKLGL
                     LHGRMTSSEKEEVINKFYKGKINVLISTTVVEVGVNVPNATVMLVLNAERFGLAQLHQ
                     LRGRVGRGMHQSYCILVNESRSKKSKDRMNVLKSTNNGFVIAEEDLKTRGPGDFFGVR
                     QHGLSKFELQSIVNNVETVEQVQLLSKELIAENPGLKGDDYLNLKLEIMKLFKDDIVF
                     N"
     gene            complement(22310..23938)
                     /locus_tag="PKK75_00865"
     CDS             complement(22310..23938)
                     /locus_tag="PKK75_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNZ82174.1"
                     /translation="MIIKYIDNVKFKRMLLGSAINLENNKTIVDSLNVFPVPDGDTGT
                     NMNLTVQSAIKEINNLQESTITAISSAAANGSLMGARGNSGVILSQLFRGIAKGLKEA
                     NLIDCKIIAESLKSASDTAYKAVMRPVEGTMLTVARETAEKAMATYEEFEDVIAFIES
                     LIDQAKDTLNRTPEMLKVLKQADVVDAGGKGLIFLMEGALAALKGVEIKQEIGEQVVI
                     DTAVELDEFTSEDEIIYIYCTEFIIKNPLRTAEEFLSRIHDKGDSIVCVGNENIIKTH
                     LHTNNPGQILAIAVKFGELTDIKIDNMREQFRERQKENNKINKNNQKRKKYGFVSIGM
                     GEGLKKVFTDLNVDVFLSGGQTMNPSTEDILEAANQINADHIIILPNNSNIILAATQA
                     REISDKDIHVVPSKTIPQGITALIAFKEENSVEDNIKAMTQAAKEVKTGLVTYAIRDT
                     VFNDLEIKKDQIIALYDGDIVNQGENPEDQAVELISKMVDEDSFLVTLFYGADVDKRT
                     AEGIKSRVEEVAEDCDIEIIYGGQPLYYYIVSVE"
     gene            complement(23952..24311)
                     /locus_tag="PKK75_00870"
     CDS             complement(23952..24311)
                     /locus_tag="PKK75_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNZ82175.1"
                     /translation="MSVKMINENGSIVIDDQALATVAGLAAMECYGVVGMASRNATQG
                     FFELLKREHITKGIKVSSAEDDKINIDLYIVLQYGVKISVVAENVISRIKYTVETFSG
                     VSVENVNIFVQGVRVQK"
     gene            24654..24845
                     /gene="rpmB"
                     /locus_tag="PKK75_00875"
     CDS             24654..24845
                     /gene="rpmB"
                     /locus_tag="PKK75_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNZ82176.1"
                     /translation="MAKYCEICGKGTISGHSITHSDRKINRTWKPNVKTITVEENGKT
                     SKKTVCTRCIRAGKVQRAI"
     gene            complement(24966..25163)
                     /locus_tag="PKK75_00880"
     CDS             complement(24966..25163)
                     /locus_tag="PKK75_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82177.1"
                     /translation="MNYFKQKKKCSPGNRPPFFKFTEILGLLIAIIGAIIIVQILPVK
                     IWMFVLGLLLIILGITLFRLL"
     gene            complement(25323..26705)
                     /gene="asnS"
                     /locus_tag="PKK75_00885"
     CDS             complement(25323..26705)
                     /gene="asnS"
                     /locus_tag="PKK75_00885"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNZ82178.1"
                     /translation="MKTTDARDIFLSPESYREVQIEGWIRTIRDSKNFGFIELNDGTF
                     QKNVQIVFENNLSNFEEVKKYSTGSAIRVKGDLVLTPEAKQPFEIKAVEIVSEAPSDP
                     SYPLQKKRHSMEYLRTIAHLRPRTNTFSAVFRVRSLAAYAIHKFFTERGFIYAHPPII
                     TASDAEGAGELFKVTTLNLENIEKVDGKIDYSKDFFGRSSFLTVSGQLEAEIFALAFK
                     KTYTFGPTFRAENSNTARHASEFWMIEPEIAFADINDNMDLAEDMVKYVVNYVLENAR
                     EEMEFFNSFVEKGLLDKLYNVVNSEFGRITYTDAVDILMKNNDNFEYPVKWGTDIQTE
                     HERYLSEKIFKRPVFAVDYPKEIKAFYMRMNEDNKTVAAMDLLVPGIGELIGGSQREE
                     RLEYLERRMDEVGINKDNMWWYLELRKYGGVKHAGYGLGFERLIMYLTGISNIRDVIP
                     FPRTPKNAEF"
     gene            complement(26717..27349)
                     /locus_tag="PKK75_00890"
     CDS             complement(26717..27349)
                     /locus_tag="PKK75_00890"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNZ82179.1"
                     /translation="MKTALIIGNGQETEASIIKNIKYDYVICADGGLEKAGNYGITPD
                     IAIGDFDSTAPDVLDEYAKKIALEKFPPEKDFTDTELAVQLAVSKGFKSIILIGASGT
                     RLDHTFGNLLLMEKYYREGVNIVIIDNNNEMKFISDKGHLLIDYKEGYYISIIPLTDL
                     EGLSLEGFKYNLNNVNVQRGSTLCISNQIKDSKGRITLRKGKAIVFISKD"
     gene            complement(27324..28001)
                     /gene="rpe"
                     /locus_tag="PKK75_00895"
     CDS             complement(27324..28001)
                     /gene="rpe"
                     /locus_tag="PKK75_00895"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNZ82180.1"
                     /translation="MKYKLAPSILSADFSNLGEAVRLVEEGGADYIHIDVMDGHFVPN
                     ITIGPAVVESLRKTTELIFDVHLMIENPDDFAGDFYKAGADIITVHQEACIHLHRTIQ
                     RIKSYGLKAGVSLNPATPVDTLREIIRDVDMVLLMSVNPGFGGQSLIKNVKYKFYDLK
                     RLIDEHKLDIDIEIDGGVNSSNLKEVLNWGANVIVAGSAIYHADDVISETRKFKEIMY
                     ENSTDNR"
     gene            complement(28012..28890)
                     /gene="rsgA"
                     /locus_tag="PKK75_00900"
     CDS             complement(28012..28890)
                     /gene="rsgA"
                     /locus_tag="PKK75_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNZ82181.1"
                     /translation="MIEGIIIKGVGGNYYVDIGSRTIECRARGLFRLKNIKPLAGDKV
                     LVRITEEDKDAGYIEEIMERKSEIKRPPVANAQQLVIIFSVINPEPSFLLLDKLLIAA
                     KSNNLDPVICINKMDLAKEGQKEYILGTFKNTGYKIIFTSKYNADSLQELKNILKNKL
                     NVFSGPSGVGKSSLMNAIEPGFYLETGEISEKLKRGRHTTRHAEIFKLSFGGYAVDTP
                     GFSSFELEGIDEYTLKNYYPEIVKYDEGCKFLDCLHYKEPGCAVKKALTDDLISEVRY
                     NNYIKLLEQIKENKPY"
     gene            complement(28906..30873)
                     /gene="pknB"
                     /locus_tag="PKK75_00905"
     CDS             complement(28906..30873)
                     /gene="pknB"
                     /locus_tag="PKK75_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HNZ82182.1"
                     /translation="MIGRILGNRYEIIEKIGGGGMSNVYKAKCNVLNRFVAIKVLRDE
                     LTQDSEFVAKFKQESLSAASLTHPNIVNIYDTGIEDDIYYIVMEYVKGETLKNYIKRK
                     TRLSEQETVKISIQVAEALKHAHINNIVHRDIKPHNILITGDGTAKVTDFGIARASTS
                     STINNTSNVIGSVHYFSPEQARGGYVDEKSDIYSLGIVMYEMITGDLPFDADNHISVA
                     MKQIQEKPVPPSKKGKNIKISKNFEDIIMKCLEKHQSFRFQSTEELLKKLNALNGNNE
                     PEKDDEIEIIDSPTIEIPALTDVEENVINIDLIDENSNSAFKSFFSGEAEDEKDEKNN
                     NNKKITIAAIISALVIAVIGGIIVFRSFLYVPEVPVPDLLGRREDEAQKLIEDLGLEF
                     KVSKREYSNEYDEGRVMEQSVAEGSKVKENYPVEVVISIGEKEILVPNLVGRYAIEAG
                     IILGEEGLLEGEATEENSDTFLAGQIIEQYPAANTPAKENDKVNYVISIGQKIIYVKM
                     PNVLNLNLETAQLSIVQHGLAVGQVNEETSEEIPEGLVMRQSVPAGDDVEQGTSIWLT
                     VSTGKPKTEEPVEPIPEEGVFPLAISLPQDRDKVSIVIQRIALDGREVVYSEEVGTSE
                     GSIIVNVKGTGTGIYEIYIDNELYDRVEIKF"
     gene            complement(30863..31621)
                     /locus_tag="PKK75_00910"
     CDS             complement(30863..31621)
                     /locus_tag="PKK75_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNZ82183.1"
                     /translation="MSMTVFFETNKGLMRENNEDSLIVEQFDNYCLYAVADGMGGHKA
                     GEVASSIAIEEIRNLFVESVGNEDFQPPSFIIESVKISNDKIREKSFSNEECCGMGTT
                     VTMAVLDKNNDIIYIGNIGDSRAYLLRNNEIKQITTDHTYVNELMKDGKITQEEAKTH
                     PKRNVITRALGSEDYVHADIFELELYKNDILLLCTDGLTTHVSDDRMLEIIKEYGTSE
                     SVQKLIKQANDNGGTDNITIIIVDNIIRGETYDR"
     gene            complement(31630..32658)
                     /gene="rlmN"
                     /locus_tag="PKK75_00915"
     CDS             complement(31630..32658)
                     /gene="rlmN"
                     /locus_tag="PKK75_00915"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNZ82184.1"
                     /translation="MRKIDDLSFEDLKDEMFIINEPKFKAGQIFDWIHKKTVDSFDEM
                     TNISQKTRAELSKEYEFTHAVIELKLESKLDETKKYVIKLEDGNIIESVFLKYKFGNT
                     ACISSQVGCKMGCHFCASTKRGFTRNLSAAEMLKQIYLIQKDTNEKISNVVIMGSGEP
                     LENFDNVVRFLNIINDERGQNISMRKITLSTSGIVPNIYRLADVNLPITLAVSLHAPT
                     QEKREQIMPIAKKYKVTDILNACDYYLKTTGRRLTFEYIMIMGFNDSREDAENLSRLL
                     KNKLANINLIPCNYIKEAGIKPSADADIMKFKKVLADNGINATIRRELGSDINAACGQ
                     LRNDLLKM"
     gene            complement(32805..34127)
                     /gene="rsmB"
                     /locus_tag="PKK75_00920"
     CDS             complement(32805..34127)
                     /gene="rsmB"
                     /locus_tag="PKK75_00920"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNZ82185.1"
                     /translation="MNSYRAQAIETLKKIFTDRSYSNLVINNDIKHVDHRFHSLYRKS
                     VLGVIENLIFIDWIIDEVSKTRTKKMETDVLLVLRLAVYQIFFLDMSHENIVVNESVQ
                     YIKDKGNISASKFVNAVLRNILRSKDSLMEKMSKLPEDGYLSVKYSYPKELVKIWKHQ
                     FGKDIEEVLTANNREATLEIRVNTLKIKRDELAELFKEKGILTHKCKYANKGLIVENP
                     FEIHKTDEYKKGLFSIQSESSMLAGQILKPEENSFIIDMCAAPGGKTLNAAEIMNNTG
                     KILSRDIYPAKLKLIENELKRLGVNNVKVEKYDAAKLDDSLIGKADYVIADVPCTGLG
                     IIRRKPEIKYNKTDVNEITKIQYKILENGSKYLKHGGELVYSTCTVNIEENNELIKRF
                     LNNNKDYKLADISENIDGFFKTAKNGYLEIYPHLHGMDGFFIAKIKRI"
     gene            complement(34179..34547)
                     /locus_tag="PKK75_00925"
     CDS             complement(34179..34547)
                     /locus_tag="PKK75_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ82186.1"
                     /translation="MKFTFNHFNFNVLDLGKSMKFYEEALGLKEVKRKEAEDGSFILV
                     YLGDGETDFSLELTWMNGRTQPYDLGDQEFHLAMAADDYDEAFKKHKEMGCIEYINEI
                     MGIYFIVDPDGYWIEVVRRK"
     gene            complement(34632..35333)
                     /locus_tag="PKK75_00930"
     CDS             complement(34632..35333)
                     /locus_tag="PKK75_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HNZ82187.1"
                     /translation="MNWEPWTGCYYVSEGCKYCYFYGPYSKRCGQNTVVKTTDFDKPI
                     LKTAKNKYKIQSGKTVATCFASDFFIPEADEWRNDAWTMIKERPDLDFMILTKRIERF
                     YVSLPGDWGDGYDNVYIGCSVENQAMADLRLPLFLSLPVKRRFVACSPLLGPVNLSSY
                     LKGIHHVTVNGESGREARLCDYDWVLDIRKQCEEFGITFWFKGTGSLFKHDGIIKKIN
                     PYKQGSIAKEFNINI"
     gene            complement(35435..36211)
                     /locus_tag="PKK75_00935"
     CDS             complement(35435..36211)
                     /locus_tag="PKK75_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82188.1"
                     /translation="MNNFKKMPLIIIIAIAAAIVLSGCFGVKIISDEPDNSTTDENEP
                     KTEASSFTFPWGGNMDGKEMTLEDVRLLAAKGDDLLFEDLWQYKGGNVSSNFERYIMV
                     YKIEGGYRLVVHSNPTGRPDAVNLESIWESGGSGIDIRYNVVDEFLSANTSQDAITEE
                     EAQDMGQSKLKVELEPVSWNILGDWPINTEDKDAVFAEALLDSIYTIDEPSWTFRVKS
                     TAEWGGSYYSVGKKTGTIFICSFDDSGKPIWSVMDSESNP"
     gene            complement(36325..36900)
                     /locus_tag="PKK75_00940"
     CDS             complement(36325..36900)
                     /locus_tag="PKK75_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82189.1"
                     /translation="MKKRNKILFSRDSECLQDLISLIQKQNHRTLVMWALDCSKLTLE
                     QFEAKYPDEHRPRICLELCKDWAKGKIKMPAAKRAILDSHAVAKEINDSQYGALCHAI
                     GHAGATVHVETHAIGLPMYELTALVLKFGRDNFEKPVIDKINYYYNRLLYWQDNAGKL
                     KLEWADFLLDDTRPNKEKLLIEKRKSKQQNS"
     gene            complement(36960..37310)
                     /locus_tag="PKK75_00945"
     CDS             complement(36960..37310)
                     /locus_tag="PKK75_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNZ82190.1"
                     /translation="MAGPVKENITFDLLDKIDIRVGTIRTVEDVAKSDKLVKLTVDFG
                     EFTRNILVGMKKERDNPKDIEGQQTLFVVNLAPKKMAGEISEGMLFDIGYADGIIPVL
                     AHPEKPVPNGTRVG"
     gene            complement(37315..38067)
                     /locus_tag="PKK75_00950"
     CDS             complement(37315..38067)
                     /locus_tag="PKK75_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HNZ82191.1"
                     /translation="MSLKEEIIELVKNSVHESESKGLFRKPIVGFSSADNPLYDQLKD
                     IVGPEHLHPNDILPNAKSVVSFFIPFSETVVKSNIKGSGVSYEWAKSYVEANSLINQI
                     SEKLADYLKNKNIDAATIKVTHNFDENTLKSAWSHRSAAHIANMGRFGLNRMIITKEG
                     CAGRFGTVIIAAELPIEKEENIEYCLYYKNGSCLKCVESCPQNALSADGFDRFKCYDL
                     LLKNADEFSHIGVCDVCGKCVVSCPFAMNIGR"
     gene            complement(38332..38940)
                     /locus_tag="PKK75_00955"
     CDS             complement(38332..38940)
                     /locus_tag="PKK75_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HNZ82192.1"
                     /translation="MMKRKVIKILALSLALSVTSIGFVFAETAENLDEPVSIQITAVD
                     DAVYEAQAEIDKLLFEQNYAGDLSEKGIFVTHTSTVDGYVEVGITPYTPENADAVIKT
                     LGKDNVRVVEGIQAVTLEYNPDSPDSIDPAELADDSQIITVTGEADDINAPAEEDVVE
                     DAKIISAPVEEVENNSISTFTILGLSALVLAGGIMLLKKKRA"
     gene            complement(39269..39487)
                     /locus_tag="PKK75_00960"
     CDS             complement(39269..39487)
                     /locus_tag="PKK75_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNZ82193.1"
                     /translation="MIYTVKSGDSLYKIAKNHNITIEDILKSNYIANPDLIYPGQEII
                     ISKEKTKTAGVEIKPKDEDDGPITIITN"
     gene            complement(39778..40557)
                     /locus_tag="PKK75_00965"
     CDS             complement(39778..40557)
                     /locus_tag="PKK75_00965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ82194.1"
                     /translation="MENLDIKDNWNRMADEYELFTDHEESYSNLIEWKAIKTILPELK
                     NKSIVDLGCGTGRFSFLFESMNPRSITGIDLSEKMIEIGLNNAKRKKSIVKFVHGNIE
                     DLSDMKLENIDFVFSSTTLHYIKNLNNIMQQINRILTSGGECILSVIHPVYSAYYPIL
                     HENRELPDDDEWTIRYLDSRDRGYVQPWIEYNPNVNNFLSFSYHHTMGDYINSIIRAG
                     FQIKELLEPLPPKSWLGNNPERYYGFINTPTYAIFKIEKVI"
     gene            complement(40837..41763)
                     /gene="fmt"
                     /locus_tag="PKK75_00970"
     CDS             complement(40837..41763)
                     /gene="fmt"
                     /locus_tag="PKK75_00970"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNZ82195.1"
                     /translation="MNIIFMGTPGFAVPSLEKLYESGHNVVLVVTQPDKPFGRGKKLK
                     KSEVKETAEKLGLEIFQPDKIKKQENIDVLKSYNPDAIVVVAYGQILSREILTLPKYG
                     CINVHASLLPKLRGAAPLNWAIINGEYKTGVTTMQMDTGLDTGDMLLKAEVEIDEEMN
                     VGDLHYILMHKGADLLIETLVKLEKNEIVPQKQDDSLSTYAPMLDNENRKINWSLPAK
                     NIHNLIRGLSPWPAAYFTMDEKTIKVYKSSYINDSTDYVPGYIIKAGKEGIFVKAKDG
                     IVILKEIQMPGKNKMTVEAYLRGNKFPENTIL"
     gene            complement(41773..42231)
                     /gene="def"
                     /locus_tag="PKK75_00975"
     CDS             complement(41773..42231)
                     /gene="def"
                     /locus_tag="PKK75_00975"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNZ82196.1"
                     /translation="MALRNLRYEGDELLRKKSREVPLVDDRIRMLMDDMVETMYKFEG
                     VGLAAPQVGILKRVIVIDVEDGKVYKMANPKIIKDSGEQTAQEACLSVPEKKGTVSRP
                     MKVTVEYTNENNETKKIEAEGLLARALCHEIDHLDGVLFVDRIIEDTSEE"
     gene            complement(42242..44446)
                     /gene="priA"
                     /locus_tag="PKK75_00980"
     CDS             complement(42242..44446)
                     /gene="priA"
                     /locus_tag="PKK75_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNZ82197.1"
                     /translation="MYNRFAQVILDNISKSTDQLYTYGVPDALLNSAEEGKRARVNFG
                     RGKSIINALIVSLDSACDYPVEKIKPLIEIIDEKPIVTKEMIKIIFWIRERYICKYSD
                     AMRLMIPSDTQSVYEKHASLKEYRETEKYKINYTPNQIKVIEYLKQHQSVSIAELREK
                     LNVSYAVINNLIKKDVLEEKPVLDYSKQEEFEPKEPLCLNEEQKRACETILRSDKKAF
                     LLHGVTGSGKTEVYMNIIEGYIKEGRQSIMLVPEISLTAQTIDRFRERFGNKIAVFHS
                     KLSKKDRYIEWLRVYNKEADIVIGARSAIFAPISDLGAIVIDEEHEDSYISATSPKYD
                     TMEVAVKMAYMAGGKVILGSATPSLNTYYMAMKGQIELIELKNRVKDASMPEMKIINM
                     SDELDKGNNTIISQELYQSIRNSLKQNEQVILFLNKRGYSGFVSCKKCGYVVRCQRCD
                     VSMTYHIKGNIMKCHYCGRTRKMIYECPKCKSDKIEQFSAGTQHVEKLINQLFPHTSI
                     ERMDLDSMTDSEAYENIYNDFKSGKIDILIGTQMLAKGFDFPNVTTVGVLSADAILNL
                     PFYTASEKTFQLITQVSGRAGRGDKKGNVYIQTYEPENFIINAARNNDYDLFLQQELK
                     LRKEFAFPPFINIINICLISKNEELVAKTAGEKYSLVKEEVQEMIKERSLLLYRPVPH
                     NIYKVNDEYRINLFIKVSNHSLPELKKILRTVYMDKDIENIKVSININTDTI"
BASE COUNT        12081 a         9455 c         6731 g        16252 t
ORIGIN      
        1 ctccaatcac ctccatatag tttatgaaat ttatcataat aagtgattaa atcacgtttt
       61 aattttttac attcgggtat atataaaata tataaattat ttaaaaagga gaatactatg
      121 attaatgaaa aagtagcaaa attaatgaat gaacaaatca acaaagaatt atattcggca
      181 tatttgtatc tggatatggc aaattactat attgataagg gcttggacgg ttttgcaaac
      241 tggtttaaag ttcaggcaca ggaagaaaga gaccatgcca tgctgttcat gcagtatatg
      301 caaaacaacc aggagaaagt cacacttgaa gcaatacagg ctccggatgt aaaatttgac
      361 agcttcgcat caccccttaa cgaggcatta aagcatgagc agtatgtaac cgcatccatt
      421 aacaatatat atgctgccgc atatgaaatt aaggacttca gggcaatgca gtttttagac
      481 tggttcatta tggaacaggg agaagaagaa aaaaatgcag atgatctgat aacaaaatac
      541 aatctttttg gcactgattc aaagggttta tatatgctta attcagaaat ggggaccagg
      601 gtgtattccc ctccatcact ggtattggat taataataag attaaaaaac acagcagtct
      661 gcctgctgtg ttttattttg tatgattttt acggtatgca cacccttgtt cctataatca
      721 aaaagtttgc cggcctcaaa tgaggatttg caataagtaa tcctgacgtt gtgaggccat
      781 tggcttcagc cactgtggaa agactgtccc ctgccttgat tacatattct ccccttgttc
      841 cctgaggaca ttgcataaaa gcttccggag gtatgcataa ttgttccccg ggtacaatga
      901 tattaggatt gaaattagga tttgtatctc ttaattcatt taagcttaaa ttgtaagaaa
      961 tgagtatgtc gcttagcctg gttccttcct gaatagtcac taccgtaccg aaaggacatc
     1021 ctgcaggaac atttggaatg catatttcct gaccaacctt caaatcatat ggatttgctt
     1081 ccggatttgc ctccaggagc agattcaaag gaatgccgta tctttgggct aaattataaa
     1141 aagtatctcc ttcccttatg gcataatagg caccgttaat gcagggcggt ctttcaggaa
     1201 cctttggaac acaaatcatc attccttctg ttaacgacct caatctcaat gtaggattca
     1261 gttcaatcag ccccactaaa ttagttccaa atctcgttgc aatggaattt aacgtatccc
     1321 ccgggcgtac ttcgtacggc attgaattta tattagggca taattgctga ttttgtctat
     1381 aaatcactat aatcacctca attataattt gttagtttat aatattcatg atatagtgaa
     1441 tgtttcctta atatgactgt tcaaactttt ttctttcctg aacagctaat ttgtcacatc
     1501 tttcgttata gctgtgaccc tggtggccgg gaacatgaac aaacttaacc ttgtggccgt
     1561 ctgcagcttt taaaagtctc cgccataatt ccttgtttaa aacttttttg ccgtcagatt
     1621 tcttccatcc cttttcctgc catatatcaa tccatccttt gttgaaggaa tccgtaacat
     1681 actttgaatc tgttatgatt gtaacatcac agggctcttt taatgcctca agtccaacta
     1741 taactcccat aagttccatt tggttgttgg ttgtattttc ttcgccccct gacagctcag
     1801 cttcatgtcc tttgtattcc aatatcactc catagcctcc cgggccggga ttgccgctgc
     1861 aggctccatc tgagtaaata cttacttttt tcatttgctt tacaccttat ttttaattaa
     1921 cattaccctt aatataccat actggtcttc cagttctttt agcttatcct cattgaactc
     1981 tccgattacg tctattatat tgcatgcata atcccctttg gatttattga ccatacctat
     2041 tatgttgaca ttgtgagatg caagggcagt tgtcatttga cctatcatgt tgggaatatt
     2101 cttattcaat acaataatcc tgtctgttcc cggatacctg tccatggagc agtcaggaaa
     2161 attaactgaa tttgtaatgt ttccattttc caaatattct gtaatctgtg tagcagccat
     2221 ttctgcacag ttttcctcgg cttcaattgt agaggctccc aaatgaggaa tacacagggt
     2281 attttttgtt cttaaaagcc tttcattggg caggtctgaa acatgatagt ctaccttgcc
     2341 attttccaaa gcttctacta aatcatcctc gttgattatt ccatggcggg caaaatttaa
     2401 tattattacg ccatccttca tggatttaat actatcccca tttattttat tctttgtttc
     2461 attattgtaa ggtacatgaa tagtaatata atcgcttttt ctataaagat cattaatgtc
     2521 aactgtataa tgaacatccc aggataatgc cagggcattt tttacggaga tgtatgggtc
     2581 atatccgaca acttccatgc ctagtttcac tcccaggttt gcaacaaggt gtcctacgtc
     2641 cccaagtcct acaacgccta aggtttttcc cttgagctcc ctgcccacaa attgagactt
     2701 tcccttttca acagtttcaa caatgttgct ttctaatttt tttgtccagt caatactgtc
     2761 tattactctt cttgagctca ttaaaagccc taaaaaagtc aattcgcaaa ctgcatttgc
     2821 gtttgctccg ggggcattaa atacaacaat accctcttct gagcatcttt caacaggtat
     2881 attgtttaca cctgcccctg ctctggctat tgccttaagc tgcttggaaa acttcatacc
     2941 gttaatatcc aggcttctca gtattattcc atctgcttct gatgtaactt cctttattaa
     3001 actgtattta tcctggggaa atagatttct tgtcacctga ggtatattac aaaatagtgc
     3061 taccttatac attggctcgt cctcctgtta tattaagaaa tagtataccg cttaaatttt
     3121 gtaaaatcaa gcgttaagtt ccaagcttat atcttataat tcagcggtgc tttagaggca
     3181 ggatatataa tatcgaagca gaaaattaag ataagggaat gctaaattta atataaatgc
     3241 atcatttaag tgccatgagc ttgttatatg aagttaaccg ttgatttctc aacggttaac
     3301 tttacttgta gctgaatgtt cttaaatctc aacagacatc ttttgtctgt tataagtact
     3361 atctgcagca tcctccccta ccgaagaaac tgtctcctcc taaaagaaaa aatataatcc
     3421 ccaatatgat taaccattca atccaatcat tgcctctgtc atagcaaccg aaatttgcag
     3481 cttttacttc atctgacata taactcacct ctcttaattt ataattcaat atatgtcaga
     3541 tagaaaaata tgtgatgact tggatttcag aaattttatt ttattttaaa ttaaagaatc
     3601 tctatggttg catatacttt ttcaattagt tctgcaacat ctaatttgct ttcagatttt
     3661 ttgatatttt caagcttagg cttcgtcaga ggatgttttg gtgcaaaaac agagcagcag
     3721 tcatcataag gcagtattga agtttcgtag gttcccactt ccctggctct ttctattatt
     3781 tctgacttgt caagacccac caggggtttg agtacaggca tatcaactgc attttcaata
     3841 actgccattg aactcatagt ctggcttgca acctgaccta agctttctcc ggtaactagc
     3901 atatctattt tattttcttg tgcgattttt tctgcaattc gcatcatgaa acgtcttgct
     3961 aatatagtgg tttcttcttc tttgcaaaat tctctgattg ctttgtgaat ttcaagtata
     4021 tttacgctgt atagttttat tttgccgcag tattcttcaa gtatttcctt taacttcacc
     4081 accttttcat ttgctctttc agatgtaaaa ggatatgtat gaaagtaaag agcacttatt
     4141 tcaactcctc ttttagcaat catatatcct gcaaccggac tgtcaattcc tcctgacaac
     4201 aaaagcaggg ctttaccgct tgaacctaca ggaagtcctc ccagagtttt tattttttcc
     4261 gaggatatat agcaggattt tcttatatca ataaagactt ccacatccgg cttatgtaca
     4321 tcaactctca cagagctgaa attctcaagg atcttacctc ctattaaagc actgaaatcc
     4381 attgatttaa atggaaagtc cttgtcgctt ctcttggtgt ttattttaaa tgtttttatg
     4441 tcctgcctgc ttaacagata tccaaacagt tcaattgttt tttcaataat cgtatcaaca
     4501 tcctttgttg ttttaacaca ggggctgata attgatatgc cgaaaacctt tctcgccttc
     4561 tcaactattt tatccatatc cccttcattt ttgctttcta taaacacctt gcctgaatct
     4621 ttgtatactg atacgtcctt aaattctttc agagcatatt taatctggtc aatgagtttt
     4681 tgttcgaaag aacctttgtt ttttcccttt aaatataatt ctccaaagct tactgcagca
     4741 atattgtaca tgtttatcct ctcctggtta tgcttcttat atctttcact gattgtttga
     4801 tactatcaat tacatattca acttcttcca ggctgttaaa atgccccaga ctgatgcgta
     4861 ttgtcccttc ccggtattcc ctgccgagat taattgcatc caatattctg ttatctcttg
     4921 acttcgaaga gcaggcagag ccggtggaaa cgtaaatata tctgtcctcc aaataatgga
     4981 ccaatacttc cccgggcacg ttcttaaaag atacacttat tatgtgaggg gctccttctt
     5041 cagggctgtt tatttttata tcttcaattt ctttaagtct ttctacatat atttttttta
     5101 agctgtaaag cttaatggtt tcctctttga aactatcata cagtaattca caggcctttc
     5161 caaagcccat aatccctgtt gtattttcag ttcccggacg gattcctctc tcctggccgc
     5221 ctccgaatac taaaggccct aactttaagg ggcttctgat atacaatcct cccacaccct
     5281 taggcccgtg aattttatgg ctgctgaagg acaatgtgtc aagagacagc ttattaacat
     5341 caattttaat tttcccgtag gattgaactg catcgacatg aagccttgca tttttatttt
     5401 ttctttttat gactgctgca atttcttcca gttcctggac tgcacctatt tcattgttaa
     5461 catgcataat tgatataaga agagtgttct cgtctaccat gtcaccaagc tcttgaatgt
     5521 ttatcctgcc cttctcatcc gtaccaagat acttaacctg gcatttatcc tcgaattgtt
     5581 tgaaaacatt gtagacggag gggtgctcga ttttagtagt tataatatta ttttgtttgt
     5641 tcaagtttga cagatgtcca aaaattgcaa tattgttgct ttccgttccc cctccggtaa
     5701 aataaatttc ctgtggtttt gcaccgataa gtcttgctgc aaatttcctg gcttcattaa
     5761 ttttcttttc ggcactaagt cccatcctgt gcagtgaaga ggggttcccg tataaatttt
     5821 cgtttgcatc tgctatttct ttcattactt cgtctcttat ctttgttgtt gcactgttat
     5881 ccaaatatac tatcatattt acctctcaat aaattatact gctttcatct caaaacaaag
     5941 caggtggcat aaaataccaa tgtttatatg gtgtttagat gtcgaaatct gtaaaaaatg
     6001 cttgcataat actgtcttgt ttgctataat tattttggtt atcccccccg ataaccgaat
     6061 atattcccaa tacccctttt atgcatatta atgcttcaca acaatggcct caaggccatt
     6121 tttgtgttta tgacctaccc aatttcttcg actgaaagga gaaagaaatt gatggtaggt
     6181 caaacgcaca gaatgccaaa tttgtgcgac gcaaggagcg aacaaatttg atgcattccc
     6241 ctgcgttgcc tgtttcattg attatgtacc ttactgtttt tctatctctt tcctgacttc
     6301 tcccatatac aatcccgata ccttcttcac aaggtcattt ccggaagaat taaattttat
     6361 attgtctatg cttcttatgg gcagcacatc attggatgtg cctgttataa acgcaccgtc
     6421 aaagctctct agttcactga atttaattat ccttttttca acttcaatta aattttcctc
     6481 acacacactt ataaccctgc ttcttgtaac acctaaaaga accgcataat ccggtgacgt
     6541 tacaagttta tcgcccttta caaaaaatat atttgatttg ctgccttcac agactgtgtc
     6601 gtcttgattc actaaaattg cttcaaaagc attttgttct tctatgatcc tggtcacctt
     6661 atccttaaat tctttttgat atgatttaac atttggattt gctctctcca ttttcactgt
     6721 aaccgtatct accccttcat tgaattcctc ctgtgcagga taataacttt caataaagta
     6781 gaataaagca aaaggcttat tgtcataaaa ataagaaacc cttatattta aattcttaaa
     6841 ggggttttct tttatcagca attctgcaga gcttttaaag tcttcataac ataagctgcc
     6901 cttgatgccg cttaagtgga tgctttcatt catacggtcg aaatgctcct tcaagaatat
     6961 ggccttcctg tcaataaccc ttataacctc ataaatcttt gtctgattca aaagcttctc
     7021 cgtttcttct tttgttataa ttttattgtt caatacattt ttatcggata ctatatttaa
     7081 catcagtttt ctacctttct tttttgaatt acataataac atcattataa aaattgtttt
     7141 acatcttctg ccgagcttac ctttttgaaa tgtttccagt ggtcgggaaa caatttcaaa
     7201 tatgaaggct gacaaagtct tgagtcaatt aataaaacgg ctcccttgtc ttcctctgtt
     7261 cttattaccc tgcctgctga ctgaagaatt ttattgaaac cgggatactt gtatgcaaaa
     7321 tcgtagccgg aattcttttt ttcatcaaag aatgctttaa ttatatttct ttcaaatgaa
     7381 acctgtggta tcccggtacc tattataact gctcctatta atttttcaag gggcaaatca
     7441 actccttcag aaaaaatacc ccctacaacc gtaaacagca ccacaccccg ttcattttca
     7501 aacctgtcta atatatccct ctgttcctcc tcattaagcc cctgattttg aactgttata
     7561 ccagtgctgt catataatga ttcatatatt gaataaacct tgctcataaa ataataggag
     7621 ggaaaaaaga ccatgtagtt tccttttttt tctgttatta aagcattgat atattcacat
     7681 gctttttcaa tatttgaatc cctcattgtg tatttcatgg atatatccga agatatcatc
     7741 aagtttaaat tttccctctc aaaaggcgag tttaatttta atatgtaatc atcttcatct
     7801 ccccccaaaa tatatctgaa atatttcaag ggagtcagtg tagcagaaaa gaaaaccgat
     7861 gattgggcat tcttttcaat ttccttcaat atagatgaag ggtctatcaa aaataatttc
     7921 accatagttt tattgtttgc tttctcagca taatagcaaa aattttcgtc agcaacttct
     7981 gaaatcttta taaaaaaagt agttttaaaa tataggtctg tcagttcatc cggaactttc
     8041 tcattttttt tgtcattgaa atatttctca gcccttgcag caaactttct taaagcgttt
     8101 gtcaattctt caggaggctc atttaatatt tgtgcttctt caacaatctt ttttatatct
     8161 atgaatttct tatttatgtt gaacaactct ttgcttaagc ttttttcgga cttcatcatt
     8221 ttatatactt cataaaattc ttctttaata atttcaggag aatacatact tcgtgcccgg
     8281 tcctccagat tatgtgcctc atctatgagt agtatatcct tgtcattatt gaaaacgtcc
     8341 tccctctgaa gtgcaactct cgggtcaaaa taataattgt aatcgcatat gacaatatct
     8401 gacatgtaag ctaaatcaag actcagctca aaagggcaca gctcatgctt ttttcctaat
     8461 tcttcaataa agtcacggtc atagaggcag tcatttttga ttgtttccct caatggaata
     8521 ttcagcttat cataataacc ttttgcatag gggcagtatt ccggttcgca ctttgtttca
     8581 tccatgaagc atattttatc cttggccgta ataattgttg tcctcagcct aaggcctttt
     8641 tcatacatca tttttaccgt gttgaaggct atattttttg tggatgattt ggctgtaagg
     8701 taaaatattt tggcgttttt ttcatacagg gattttactg caggaaataa tacagatact
     8761 gtcttcccca cgcctgtggg ggcttgagcg aagagctttt ttcctgacct gattgacagg
     8821 aaaacagcaa ctgcaaaatc tctttgtcct tttctatagt catcaaaagg aaaaatggtt
     8881 tccttaattg ttttatttct ttccttttga agctttatga ttgtttctgc ccagtctatg
     8941 tatgtattta gtaaatcata gaaaaatgtt tcaagttctt ctgttgtaaa tgtctgtttt
     9001 attatctttg tttcattgct gtcaagatta tagtatctaa gctgcacgtt gagtttttgc
     9061 agtctgttta taaatccgta catataggca tagcatttag cctgtgccat atgggcaacc
     9121 ttgtattcat catcgatata acttaaatct gtatatgttg attttatttc atcgattgtt
     9181 atttcatcct cttcaattat tatcccgtca gcccttcctt caattgaaaa aacaatatca
     9241 ttaagaatga attcgctttt aagatagaat tccttcaggt agttttcacc catttgactc
     9301 tggagaattt tatgagcttt gataccatcc aatgctctgt ctgtacttaa attcttaacg
     9361 tttatatctc cgcttttata aacaaattca accaattccc ttacggaaag ctttatgttg
     9421 tccaatttaa aactcctcaa aaataaataa acaccgcagt gtttattata gcaaattttg
     9481 taatttattt aaatgtttat tttaaatatt gcacacaagt aacggtattc ccatcaatat
     9541 ttaacttgtc ggaaagtccc tccaccagca tcagccctct gccggattcc agataatcat
     9601 attctccgca atctttcagg tcatagcaaa tgcctgagcc ttcgtcggaa acctcgatta
     9661 tgactcgtga tttgttaatg taaacattta tatttatcat tttattcaag tctccacaat
     9721 taccgtggat gacgctgttt atcatcaatt cataaagaat aagctttgta ttgaaaaaag
     9781 tatcagggtc aagtattccc ttaatatttc ttaagatttc ttcaataatc tttttaccgc
     9841 atttaatgtc gctgttgact tttctttgaa ctacataatg catacctatt cctccttatt
     9901 gtaatatacc ctttttttat gtgcctaaac agccaaatat attaaaatta aagaggaaca
     9961 tattgtaata caatgatttg tctaaatctg cagatttaaa atattttcaa tcaatatcca
    10021 tatttcttct gctccaagct tagatgttga agaaaatgga aggattaaag agctgtcatt
    10081 tatctgcatt gtctttttaa taagacttat gttttttaat tgttgagacc ttgaaagctt
    10141 atcagctttt gcagctataa cataaccttt aaatccaaca ctttttatcc attcatacat
    10201 ctgcaggtca tttttcccgg gttcatgtct tatatctaca agtagaataa cttctttaag
    10261 ctgctgcctt ccatgtaaat atgaatcaat catggctgcc cattttaact gttcattctt
    10321 tgatactttt gcatagccgt atccgggaag gtccacaaaa ttgaactcat cgttaatatt
    10381 gtagaaattc aaggtttggg tttttccggg ctgggaactg gttcttgcca ggcttcttct
    10441 gtttaaaaga gcatttatca tggaagactt ccctacattt gatttgcctg caaatgctat
    10501 ttcaggaact gttgtgtccg gatactgctg tttctttact gctgaaataa tcatctcagc
    10561 tttcttaata atcatataaa tcctttcttt tacaataaca aacgaaaatg actgttttaa
    10621 tatatatatt tcacttgtat tagataatag tatctaagct gcaaattgag attatgaaaa
    10681 ataataaaaa tatcgctcac ggtttcggac acgaataatt ctaaagctca ttccggtcaa
    10741 aaatcctaca gcttgcaaac tcactatgtt caaacagtgc aagctgttaa cggatttttg
    10801 tcctccatcc gctaagaatt ataatcgtgt cctgcaaatg ttcgcttaca tttttattat
    10861 ttttcagttt caggctgctg tactgcttct tttaccacat catctatttt gtctgcatat
    10921 actatttcca ttgaacggat tatcttcggg tctacttttg aaacatccgc cttgttcttg
    10981 gaacacaaaa caaccttctt tatacctgct ctctttgcag ccagaagttt ttccttaaga
    11041 cctccaatgg gcaaaactct tcctgtgatt gtaatttcac ctgtcatcgc cacgtccttt
    11101 ctaacaggct tctttgtcaa ggctgatatt attgctgttg ccattgttat tcctgcagaa
    11161 ggtccgtcct ttggaattgc accttcaggc acatggacat gtatatccgt ttctttaaat
    11221 atttgatagt caatgccgaa tttttccgca ttggccttta tatagcttaa ggcagccatg
    11281 gcagattcct tcataacatc gcccagctga ccggtaagct gaagttttcc gtcacccttc
    11341 atcgtgttta cttcaacaaa cagtgtttct cccccaactt gagtccaggc cagtccagtt
    11401 acaactccaa cctgatcttc ttcataagca gtatcgtaat cgtacttagg tatgcctaag
    11461 tattttttga tgtttgaatt atttatggaa acagactttt tgtttttttc aacaacttga
    11521 actgctgctt ttctaattaa tttgccgata tttctttcca ggtttctgac tcctgattcc
    11581 cttgtatatc ttccgattat ttcatctatt gcatttgttg aaatattaac ctggtcttca
    11641 ttcagcccat gctcggaaag ctgtttttta attaaatatt tttctgcaat attccttttt
    11701 tctacatcag tatatcctga tatttcaata acttccattc tgtccagaag aggtccggga
    11761 atggttgccg tggtattggc agttgtaata aacattacat tctgcaaact gaagggagct
    11821 tcgatatagt ggtcggtaaa tgtagagttt tgttcaggat ccagagcctc caataaggct
    11881 gatgccgggt cgcctttaaa gtcgccgttc agtttatcta tttcatccag caaaaataca
    11941 ggattattct cctttacttt tgcgatggaa gatattattc ttcctggtat tgcacctata
    12001 tatgttcttc tgtgtcctct gatttccgcc tcgtccctta ctcctcccaa agaaatccgc
    12061 acatactttc tgtccatagc acgtgcaatt gattttgcta ttgatgtttt tcctactccg
    12121 ggaggtccca caaagcataa aatgggtccc ttcatatcct ttttaatatg ttttacagct
    12181 aaatattcca gtattctttc tttgacttct tccagcccgt aatggtcttc ctcaagtata
    12241 cttcttgcct ttgacaggtc cgtgttatcc ttagtctttt tatcccaggg caggtctata
    12301 agccagtcaa cataggttct tattactcct gcatccggag aggaaggaga aattttcaaa
    12361 agcctgttta tctccttctc tgctttttct ttaacgtcct taggcatatg ggcttttgct
    12421 attttttgga ggtactcttc cacttcatag tcagtgcctt catcctcacc taattccttt
    12481 tgaattgctc tcatctgctc ttttaaataa tagtcctttt gaaattcact gacttgtttt
    12541 tttacaagct cgcttatttc attttctatc tggagtacct taatttcttt tgccagaagt
    12601 ttatttattg attcaagtct cttatagggg tcaaatattt ctaagagaga ctgtttttgg
    12661 tttgttttca gataaatata tgacgcaaca acgtctgcca gcttatctgg attttttatt
    12721 tcctttaatg atattactgc gtccggtgct atttttgcat agattaaagc atattcttca
    12781 aaattgttca aggtgaggcg gaccatagcc tccagattat catccaccgg aaggctgtca
    12841 tcataaatat actcatccag gacgacctca aaataggggt cattctgagt tatggattca
    12901 attttagccc tgtttatgcc ttcaaccaat actcttatat tgtccccggg cattttaatc
    12961 atctgcttga ttttgcagat aacaccaact tcataaaaat catcttcctt tggggattcc
    13021 atgtcagcaa ctttttgcac agtcaaaaaa atgtctgaat cctcgagcat tgcttcttcc
    13081 agtgcgttga ttgacttttc ccttcctatg tcaaaatgta tcaccgtatt cgggaatatc
    13141 cctattcccc ttatgggaat caatggaagg tttcttctct ctattatgta attgttaatc
    13201 atactttctc ctttgtgcgc aggcttcata tcctacatat gggtgcacag ggaaattcac
    13261 taatttattt gatccttacg ctgcataaat taggaatttt gtgcgtttga cctaccagcc
    13321 attcctgctt cgctatcgct tacagcaata aggttaagtc ataaaggtta ttaaaactgc
    13381 ctgctaaggc aggcagtcta tgaagcattc tccttcttat cggaaacagg cttctttgtt
    13441 aatactaatt tcggctcgct gttgtttgtg attgtatcct ttgtgataac acacttttta
    13501 atatcgcttc tcgaaggaat ttcatacata acttcattca ttatgttttc aagaattcct
    13561 cttaatcctc ttgcacctgt gtttatttca agagctttct ctgctattaa ttcaagggct
    13621 tccttctcta tttcaagctc cacattatcc attttaaaca attccttata ttgtttcagc
    13681 aaggcattct ttggctctgt caaaattgat atcaaggctt tcctgtctaa tttctccaaa
    13741 gctaccacaa ccgggaccct tcccacgaac tcaggaatca ttccatattt cagcaaatcc
    13801 tgagactgaa cctgcttgag cagcttatcg gtatccattg ccgtatttcc cgaaatgtca
    13861 gcgccaaaac caatgttttt cttgcccact cttttctgta cgattttctc catgccgtca
    13921 aaagctcctc caagtataaa caatatattg gttgtatcta tctggataaa ttcctgatgg
    13981 ggatgttttc tccctccctg gggaggaaca ttggcaactg tcccttcaag tatcttgaga
    14041 agcgcctgct gaactccctc gccgcttaca tccctcgtta ttgaaggatt ctcagaccgt
    14101 cttgaaattt tatctatttc atcaatgtat attataccct tttctgcttt ttccacatca
    14161 aaatctgcag cctgcaataa ttttagcaat atgttttcca catcttctcc gacatatcct
    14221 gcttcagtca aggaggtggc atcagctatg gcaaagggta catttaataa ttttgccagt
    14281 gtctgtgcca gatatgtttt tccgctgcct gtaggtccaa gtaagagtat gttgcttttt
    14341 tgtatgtcta cgccttcgtc cttagctttg tagtttattc ttttataatg attgtatacc
    14401 gcaacagcca gggccttttt tgcattatcc tgttgaatta catactcatc caatttttct
    14461 tttatttctt taggctttgg cagttctgta aaatcatatt cttcaaatat atcaacatct
    14521 tcgtctacga tgttatggca caattctatg cattcatcgc atatataaac gtccggcccg
    14581 gctatcagtc ttcttacctg ctcctgagac ttgccgcaaa acgagcattt aacatgtttt
    14641 tcatttactt tgcccattaa aaacactcct tattttcttt taactatgat atcatctatt
    14701 atgccataga ttttagcttc ctccgcagtc ataaaattat ccctgtctgt gtcctgttca
    14761 accttggaaa tgggctgacc ggttttttcg ctgattattt tatttaatgt atctcttatc
    14821 tttataattc tgtcagcatg tatttttatg tctgaagcct gcccgctcat tccaccaaga
    14881 ggctgatgaa tcataatttc tgaattagga agagcaaatc tctttccttt ttctcccgac
    14941 aggagcagaa atgcccccat gcttgctgcc attccaacgc aaattgttga tacatcgggc
    15001 tttactaact gcattgtgtc atagattgcc atgcctgcag ttatagaccc tcccggtgaa
    15061 tttatataaa attgaatatc cttatcaggg tcttccgact ccaaaaataa catctgtgca
    15121 actactaaac tagctgtcac atcattgact tcatcactca gtattatgat tctatctttt
    15181 aaaagtcttg aatatatatc gtaagatctt tcacctctgc cggtttgttc gattacatac
    15241 ggtactaatg ccatcttctt tcctcctata ttatattcat tatactttaa ttgtacttat
    15301 ttttcttaaa tttatcttaa tctttattcc ttgtctttta tatgctctgc tgctcctgca
    15361 ctttcagccg cttccactgc tgcgtcagtt tcagtttttt caggggcatc aacaaataca
    15421 gcattttcca caagcatatc aatagctttt cttttttgaa gggtttcttc aatatagctt
    15481 ttgcttgact gcattaatcc ctctttgaat gcttcaagtc tttcttcttc aacattgtat
    15541 gactctgcta ttttattcac ttcttccttg aattcttcct cggaaacaga aattttttct
    15601 ttttttatca atgcttccag taccagttca gctttaacat tgaactttgc ctgttccctt
    15661 gctccttcct tgtactgctt tacgaaattc tctatcagct catcgtattc tttgccgttg
    15721 aatccctgct gacggaactg ctgttcgtaa tttcttccaa ggaggtctac ttctctctgg
    15781 attaaaactt ctggtatttc cagttttgta tcatttacta actttgtaac aacattattg
    15841 tcattggaaa ttttaaccct gtcagcagcc tttttctcca atacacttct tgtatctgcc
    15901 ttaagttctt caagcgtgtc aaattcactc acatctttag caaattcatc atccgtttca
    15961 ggaagttctt ttactttaat ttcatgaatt ttcacattga agaccgcatc cttgcccttt
    16021 aaattttctg catgatattc tacagggaat gttacattta ctgtgacttc ttcacctttg
    16081 ctctttccta tcagctggtc ctcaaatccc ggtataaatg aattagagcc tatttccagt
    16141 gtatggtttt cggctgtgcc cccttcgaat tgctcatcac ccacaaatcc tgcataatca
    16201 atagttaaaa agtcgccctt ttgaacaggt ctgtcatgag cttctactat tcgtgcattt
    16261 ttctcctgta ctgactttaa ttctctttca acatcttcgt ctgttacttt tatttctgtc
    16321 ttgactactt caatgccttt gtattccgga agctcaacat ccggcatcac ttcaacttct
    16381 gccttgaata ttaagtcttc gccctcctca aatttttcaa catcaatttt tgggctgtcg
    16441 attatatcca gttccagttc ttttacagca gcaggatatg cttcctgtac tgctatatcc
    16501 atagcatcat cataaaaaat gccctttcca tatttttttt cgataatatg tctcggagct
    16561 tttccttttc tgaatccctg aacatttata ttattctttg cttttaaata tgctttattc
    16621 acagcctttt caaagtcatc ctgcgatact gtaaattcca aggttacagt attattttcc
    16681 ttttttagta cttttgaact cattttgccc tccgctatcc ttaagtttat atgtaatttt
    16741 tatttgatac taaccaaatt ttagaattaa ccactatatt ataccacaaa acatccttaa
    16801 agcaaggatt ttttgtactc atagagcctg catttcatta ttatattatt gctgtttaaa
    16861 taaatttgag ttttgttctt cacaaattgc atgccggctt ttttcataac tttacctgat
    16921 gcttcatttc ttatgtcatg aagagcacaa atcctttcaa atcctgcttc ctgtgtcaga
    16981 taattcaggg tgcagattaa tacttccgtt gcaattcctt tattccacag tttttttgct
    17041 attgtgtaac ctatttcaca atattttttt ctttttttta tgttcgatac tgtgacagaa
    17101 cctattactt catctgtaac cttgtcagca acagcccagt ggtagtaatc atttttttta
    17161 taaagactta tccactcaga cagatactct tttgttactt caattgacga atggaccctc
    17221 caggcgttat atctggctga atcctcgtcg ctcagccaat tggaatatat gtcatgtgca
    17281 tcagacattt gaaattttct gagtaaaaat ctttttgttt ccagctgaac agttcctgaa
    17341 agcactaatt tttccatata tcctccgcta tatactagtg atattataac ttttattggt
    17401 gtatttgcat agctttattt ttattagttg aataataccc aaaaaaatag tataataaac
    17461 aaaaaaagga gctgcatatg ggtgataata tagaaattat aattgacaat tacagtgaag
    17521 ctgttaaaac aaatactgaa gtcaaagata aaaagtttgt acccacaatt gaatctgtcc
    17581 ttaaaaagaa acatatacaa atgcctcagg aaatatacaa tgccagcggt ataaaagttt
    17641 tcgggaaaag aataaaatca ttgatttata ctactgattt agcaataata aaaaacaata
    17701 atgcagacgg agttattgcg gtatacccct ttacccccca aatagctatt aatcaggcaa
    17761 ttattgagct gtcttccaca cctgtattcg taggtgtagg aggaggtacc acaacaggac
    17821 aaaggtcaat tgacattgcc ttgaatgccg aattaaccgg agcatacggg gtagtattga
    17881 atgcaccaac taaaaacgaa cttatatccg aaatgaaaga gcgtttggat ataccgatta
    17941 taattaccat agtatctgaa aaagaggact ttgaatcgag aataaatgcc ggagtttcaa
    18001 ttttcaacgt ttccggagga gcaaatacag caagaattgt aaggcaaatc agggaaaaac
    18061 atccgagctt tcctataatt gcaacaggag gtcctacacc ggatacaatc ctggaaacaa
    18121 tacaagcagg cgccaataca ataacataca cacccccttc aataggtgat attttctctg
    18181 aaatgatgga agtatacaga catagaatca tcgatgaatt agataaaaaa aataaataat
    18241 aaacttataa ccctcccgat atttacagtt ttcgcggagg gttattttat tgattattta
    18301 cattttttta agtacgtctc tgttgtcttc tattatgtta tttatttctt tgattttatc
    18361 ctggagtctg gacaatattt catcactgta attatatgcc cctgtcttca tttcgtttgc
    18421 tctttgctgg gcttcctgaa ttaattcatt agcataattt ttagcttgtc ttgtaatttc
    18481 gctttcttca atcaaatgct tctgctgttc ttttactttg ataaccagct catctgcttc
    18541 tgtttgagcc tcattgagta ttcgctgcct ctccttggca acccatgaag ccctgtttat
    18601 ttcatcaggc agtttcaatc ttatttcgtt aattacttcc aacacttctt ctttgtcaac
    18661 cataacctta tttgataagg gaaatttaga agcatcttct ataatatcct ctatcctttc
    18721 caataaattc aaaatatcca ttttacttcc tcccgagtaa tctatatata ttttgtacaa
    18781 cattttcagg aactaaatct tcgaaacgcc ctccgtgttc tgccacgtcc tttaccatgc
    18841 ttgagcttaa aaatgaatat tggggtgtag caaccaggaa aaaggtttct aattctttgc
    18901 ttaaatgctt attaatgctt gatattgaca gctcatattc atagtcagac actgctctga
    18961 gacctctcac tataatactt atgtccttcc tggcacaata atccacaacc aagccgctgg
    19021 taaaatcaac ctctacgtta gttaagcctt caactgcagc tcttaccatt tcgcatcttt
    19081 cttctatggg aaaccagtat tttttttcct ggttattaaa tacagtaact ataaccgtat
    19141 caaatttttt tgatatcctt ttgatgatat caagatgtcc aactgtaatg ggatcaaaac
    19201 ttcccgtata caatactctc attaactcat tttccctcca aaagtcttat tatgcttatc
    19261 tttgttcttc cgtatatctt ctctttgtat tctaccacat ctaaggcctc gttatcaatt
    19321 actttttctg atttttcact ttctatgaca agaatccccc cgtattttaa aataccgttt
    19381 tcaataacag catttaagat tttattgcct aaggtaagcc catagggagg atctgcaaaa
    19441 atatagtcaa acttttcctt gttatttgat aagtttatta tcgtcttttc ataatcacct
    19501 gctattattt ttgctttacc ggtaaaatta cacaaatcta aattcttttt cacatagtat
    19561 aaactttttt ttgatatatc tacaaatgtg caatgctttg cacctcttgc cagaaattct
    19621 atacccatgc ttcctgagcc ggcaaataac tccagaacag ttgaatcttc atcaatggaa
    19681 cctataatgt tgaaaaccgc ttctttgatt ttatcgccgg taggtcttac ctcttttcct
    19741 tcaggggcat agagcctctt tcccttgttt gttcctgata taattctcat tttatttcct
    19801 ctttatcaat ggtatgggaa acaatacagt tatgattagc cctgggggta aaagcaatgt
    19861 cccggattat gcgtcaatta aaaactatgt catctttgaa caatttcatt atttctaatt
    19921 ttaagttaag gtagtcatcc ccttttaaac cgggattttc agctataagc tcttttgaca
    19981 aaagctgaac ctgctcaaca gtttcaacat tattaactat actttgtagt tcaaactttg
    20041 ataatccatg ctgccttaca ccgaagaaat ctcccggtcc ccttgtcttt aaatcttctt
    20101 ccgctattac aaagccgttg tttgtactct ttaaaacatt catacggtcc tttgattttt
    20161 tgctcctgct ttcatttacc agaatgcagt aggactgatg cattcctctt ccaaccctgc
    20221 ccctgagctg atgaagctgg gccaggccga acctttcagc attcagaaca agcataacgg
    20281 ttgcattagg tacatttacc cctacctcaa caaccgtggt cgaaataagc acatttattt
    20341 tccccttata aaatttattt ataacctctt ccttttcgct gctggtcatt ctgccgtgca
    20401 aaagacctaa tttatagctt gaaaaatacg aggatttcaa ttctttaaaa atttcaacag
    20461 cggaatttaa gtctagattt tcagattctt caaccagggg cgctacaata tatgcctgtc
    20521 tgccgctgtc agcctgtttt tttataaaat cataagcttt ttccttttcc ttgccgctta
    20581 taatcgatgt tatgaccttt tgccttccgg gaggcatttc atctatcaat gaaatatcca
    20641 aatccccgta aatcataaga gccagtgttc ttggaatagg tgttgccgtc attatgagaa
    20701 tatgaggagc ttttccctta tttgacagcc ttgctctctg ccttacgcca aaacggtgct
    20761 gctcgtctgt aaccacaagc ccgatattat taaattccac attgtcttca ataagtgcat
    20821 gggtccctac aataatgtca atttctccgc ttttcagctt ttggagtatt tcttctttgt
    20881 cctttggctt cacagaccct gttaacactt caatttttac atccaaatct gctttttcaa
    20941 taaattcttt tatcgtttca taatgctgga gtgctaaaat ttccgtgggt gccatcatgg
    21001 atgactgata tcctgaggct gcagccataa gcattgcata gacagccacc acagtcttgc
    21061 ctgagccaac gtctccctgt ataagtctgt tcattgtctt ttgacttttc aagtcttctt
    21121 ttatctgcga tatgaccttc agctgtgcat ttgtaagcgt gtatggtata gacttgagaa
    21181 gcttttctga aagccctgtg tttcttattt ctatatgact tacaccattt gataaattat
    21241 tcttaatgtt aaaaagacca agctgcatta tcaataattt ttcaaaggcg atagttcttt
    21301 ttgccagtga atatttcttg ttgtcgcttg gaaaatgaag agcttttaca gcaattttct
    21361 ttgaggtaag cttgtatttc tttattattt cttcaggcag aatattttta atttcatcat
    21421 agtatgtttc taaagcagtt tttaccagct tggtaatttc attgtttgac aatccataag
    21481 taagaccgta gacaggcata atgtttcctg ccttccaatc cttatccaca ggttcgattt
    21541 caggatttac cgcttcaagt tttccgtaag ctgttttaat attaccgaaa atatagtagt
    21601 actcaccggc aataagctta tcctttaaaa aatcctgatt gaaaaatgta agagtgataa
    21661 aacccgtatc atccttacat attgctttta ttatagtcat cccttttttg attcttgaat
    21721 atttgggttt ttcaaaaatc ctgactttta acaggtattt gccctgttca catacttcgt
    21781 ttatattttt tattgttctt cgatcttcat atttaagagg aaaataattc agcagatctt
    21841 ccactgtaaa tatgtttaac ttttgtagtt ttaaagctct ggccggtcct actcccttta
    21901 aatattgtat atctgcatct aacatatttc accatttatc atacagcttc atttaccgat
    21961 tgcatattca gggacattta tcatgcccta agataccaaa tagggttcga aggtaaatcc
    22021 accggtttat ttgtcagggc atctcgcagt ctcgctgcac aatcttgttt gctccttgcg
    22081 tcgcacaaaa ttggcgctcg ttgcgaatga cttaccgcta ttttttctgc gcttaggctt
    22141 gaaaaaatag ggttcgaagg taagtccacc ggtttatttg tcagggcatc tcgcagtctc
    22201 gctgcacaat cttgtttgct ccttgcgtcg cacaaaatcg gccctcgttg cgaatgacct
    22261 accgctattt tttctgcgct taggcttgaa aaaatagggt tagggcatct tattccactg
    22321 acactatata gtaataaagg ggctgacctc cgtatattat ttcaatgtca cagtcttctg
    22381 caacctcttc aactcttgat ttaatacctt ctgcggttct tttgtcaaca tctgctccat
    22441 agaataaggt taccaaaaaa ctgtcttcat caaccatttt acttatcagt tcaactgcct
    22501 ggtcttcagg attttctcct tggtttacaa tatcaccgtc atatagggca ataatttggt
    22561 cttttttgat ttctaagtca ttaaacacag tatcccttat ggcataagtt acaagcccgg
    22621 ttttaacttc ctttgctgcc tgtgtcattg cttttatatt atcttccacg ctattttctt
    22681 ccttaaacgc aataagggct gttattcctt gaggtatagt cttgctggga accacatgga
    22741 tatctttgtc ggaaatctcc cttgcctgcg ttgccgccaa tataatattt gaattattcg
    22801 gcagtattat tatgtggtct gcattgattt gatttgctgc ctccaatatg tcttccgtac
    22861 tgggattcat tgtctggcct cctgacaaaa acacatccac atttaagtca gtaaagactt
    22921 tttttaatcc ttcccccata cctatggaaa caaatccgta tttctttctt ttctgattgt
    22981 ttttattgat tttattgttt tctttttgtc tttccctgaa ttgttccctc atattgtcta
    23041 tttttatatc agtaagctca ccgaatttaa ctgctattgc caatatttga cctgggttgt
    23101 ttgtatgaag gtgtgttttt attatgtttt catttcccac acaaactatg gaatcaccct
    23161 tgtcatgaat tctgcttaaa aattcttctg ctgtccttaa tgggtttttt attataaatt
    23221 cagtgcaata tatatatatt atttcatcct cgcttgtgaa ttcatcaagc tcaaccgctg
    23281 tatctatcac gacctgctcc cctatctcct gttttatttc tactcctttt aatgctgcaa
    23341 gagccccttc cataaggaag ataagccctt ttcctcctgc atccaccaca tctgcctgct
    23401 ttaaaacctt aagcatttca ggagtccggt ttaaagtatc ctttgcctga tcaatgagac
    23461 tttcaataaa agctataaca tcttcgaatt cttcatatgt tgccattgct ttttctgcag
    23521 tttccctagc taccgtaagc attgttcctt caacaggtct catgactgcc ttgtaagcag
    23581 tatctgatgc tgatttcaag gattctgcga ttattttaca atcaatcaaa ttagcttcct
    23641 taagtccttt tgcaattcct ctgaaaagct gtgacaggat tacacctgaa tttcctcttg
    23701 cccccataag ggacccgttg gctgcagcac ttgaaatggc tgtaatcgtg ctttcctgaa
    23761 ggttgttaat ttccttgatt gctgactgta ccgtaaggtt catattagta cccgtatctc
    23821 cgtcaggaac aggaaatacg tttagtgaat ccacaatagt tttattgttt tcaaggttaa
    23881 tggcagagcc caaaagcatt cttttaaatt taacattgtc tatgtacttt attatcattc
    23941 tagcctcccg gttacttttg caccctaaca ccttgtacaa aaatatttac attttccaca
    24001 gatactcctg aaaaggtttc aacggtgtat ttgattctgg aaattacatt ttctgcaaca
    24061 actgatattt taactccata ttgcagtact atatataaat ctatattgat tttatcatct
    24121 tctgctgatg aaacttttat cccttttgtt atatgttccc ttttcaacaa ctcaaaaaaa
    24181 ccctgagttg catttctgga agccataccc acaacaccgt agcattccat tgcagctaaa
    24241 cctgcaactg tagccaaggc ttgatcatca ataactatac ttccattttc attaatcatc
    24301 tttactgaca tttttacctc ctgttttaat ttattattta atttattata acctctttgc
    24361 catgaattta caaataatat gctaacctgc catttggaca tttttatttc ccaagacacc
    24421 aaatcgggtt cacaggtata ttcgccaatt aatcagctgc taacgccgca caaattgcaa
    24481 atattatgtg tttgcctacc gctatttttc tatccctccg gccgaaaaaa agggttagga
    24541 catcttcaat tatactaagt aaaaattaat attgcaatat ataattttta atgttagaat
    24601 aatgaagtta aaataaaaga ctgtatatta tggtatatat gggaggtgga attatggcaa
    24661 agtattgtga aatatgcggg aagggaacta tatcaggaca tagcattacg cattccgata
    24721 gaaaaataaa cagaacatgg aaacctaacg taaaaacaat cacagttgaa gaaaacggca
    24781 aaacttcaaa aaaaacagtt tgtacaagat gtataagagc aggaaaagta caacgtgcta
    24841 tttaggcaca tctatcatta aaatagtggc aaaagccatt atttttttgt gtccctctca
    24901 aaacggaaca ttcctttatt tgttctcccg ctaagattat ctgtcataga ggtatgtccc
    24961 ttgtcttaca acaacctgaa caaggtgatt cctaatataa tcaagagtaa tccaagaaca
    25021 aacatccata tcttaacagg cagaatctga actatgatga ttgctcctat tattgcaatc
    25081 aagagaccca gtatttctgt gaatttgaaa aatggcggtc tgtttcccgg actgcacttc
    25141 tttttttgct taaagtaatt cattatgtcc cccttagaaa tttaaatctc actattaata
    25201 cattctattc aggtagcaaa aaaaaataca aagaaatttt ctttgcatct ttcttattgc
    25261 tatcacagta tccttagaaa aatgataaaa atgttcgctc gcacttttgt catttttccg
    25321 agttagaatt ctgcattttt aggagttctg gggaatggta ttacatctct tatgttgctt
    25381 atacccgtca aatacattat cagtctttca aagcccagac cgtatcctgc gtgttttaca
    25441 ccgccgtact ttcttaattc caggtaccac cacatattat ccttgttgat tccaacttca
    25501 tccatacgcc tttcaaggta ttccagtctt tcttcccttt ggctgccgcc gattaattct
    25561 cctattccgg gaaccagcag gtccattgcc gctacggttt tattgtcttc gttcattctc
    25621 atataaaatg ccttgatttc cttgggataa tcaactgcaa atacaggacg tttaaatatt
    25681 ttttccgaca ggtatctttc atgctctgtc tgaatatctg tcccccattt aaccggatat
    25741 tcaaagttgt cattgttttt catgagaata tccacagcat ctgtatatgt tatcctgcca
    25801 aactccgaat tcacaacatt gtagagttta tccaaaagcc ccttttccac aaaactgttg
    25861 aaaaactcca tttcttccct ggcattttcc agaacgtaat ttactacata tttcaccata
    25921 tcctctgcca gatccatatt gtcatttata tcggcaaaag caatttcagg ctctatcatc
    25981 caaaattccg aagcatgtct tgcagtattt gaattttcag ctctgaatgt aggtccgaag
    26041 gtgtaggttt tcttaaatgc aagtgcaaat atttcagcct ccaactgtcc gctgacggta
    26101 agaaatgagg acctgccgaa aaagtctttt gagtagtcaa tcttcccatc aaccttttca
    26161 atattttcta aatttagtgt ggttactttg aacaattcac ctgcaccttc tgcatctgaa
    26221 gcagttataa taggcggatg tgcatagata aatcctcttt cagtaaaaaa tttatgaatg
    26281 gcataagcag ccaggctcct tacccgaaaa accgctgaaa atgtgttggt gcggggtctt
    26341 aaatgtgcaa ttgtccttaa atattccatt gaatggcgtt tcttctgcaa aggatagctt
    26401 gggtctgatg gtgcttccga aactatttca actgctttta tttcaaatgg ctgctttgct
    26461 tcgggcgtta aaaccaggtc gccttttacc ctgatggcgc tgcctgttga atattttttg
    26521 acctcttcga aattgcttaa attgttttca aaaacaatct gaacattttt ttgaaatgtt
    26581 ccgtcattta attctataaa cccaaaattc ttggaatctc ttatggttct tatccagccc
    26641 tctatctgga cttccctata actttccgga gatagaaata tatctcttgc atctgttgtt
    26701 ttcatgtgtc ctcctgctaa tcctttgata taaatacaat agccttccct tttcttaagg
    26761 ttattcttcc cttactgtct tttatttgat tgctgataca aagagtagag cctctttgta
    26821 cattgacatt gtttagatta tatttgaatc cttccagact aagcccctct aaatctgtaa
    26881 gagggattat ggatatgtaa taaccttcct tgtaatctat gagtaagtgt cctttgtctg
    26941 aaataaattt catttcatta ttgttgtcaa ttatgacaat atttacacct tctctgtaat
    27001 atttctccat gagaagaagg ttgccgaatg tatggtctaa tcttgtgccg gatgctccta
    27061 tgagtattat acttttgaat cccttggaaa ctgccagttg aacagctaat tccgtgtccg
    27121 taaagtcttt ttcaggaggg aatttttcaa gggctatctt ttttgcatat tcgtcaagaa
    27181 cgtcaggagc tgtggaatca aaatccccta tggcaatatc aggagttatt ccgtaattcc
    27241 ctgctttttc gagccctccg tccgcacata taacataatc gtactttata tttttaataa
    27301 tgcttgcttc ggtttcctgt ccgttaccta ttatcagtgc tgttttcata cataatttcc
    27361 ttgaattttc tggtttctga tataacatca tcagcatggt aaattgcaga gccggcaact
    27421 ataacatttg cgccccagtt taaaacctct tttaaatttg aggaattcac tcctccgtct
    27481 atttcaatgt caatatccaa tttgtgttca tctattaacc ttttaaggtc ataaaattta
    27541 tacttgacat ttttaatgag gctttggcct ccaaaaccgg gattcacgct cataagcaaa
    27601 accatatcca catcccttat gatttccctt agagtatcta cgggtgtggc aggattcaat
    27661 gatacccccg cttttaagcc gtatgatttt attctttgga tggttctgtg aagatggatg
    27721 caggcttcct ggtggacggt aattatgtct gctcctgcct tgtaaaaatc ccctgcgaaa
    27781 tcatccggat tttcaatcat caggtggaca tcaaatataa gttctgttgt ttttcttaaa
    27841 ctttcaacaa ctgccggtcc tattgtaatg ttgggaacga aatgcccgtc cattacatca
    27901 atatggatgt aatctgctcc tccttcttcc acaagcctta cagcttcccc taaattagaa
    27961 aagtcggcag ataatattga aggggctaat ttatacttca tcttctcctc cttaataagg
    28021 cttattttcc tttatttgct ccaaaagttt aatatagtta ttatacctta cttcactgat
    28081 taaatcatca gttaaagcct tttttactgc gcagcccggc tccttgtaat gaagacagtc
    28141 caaaaattta catccttcat catatttcac tatttcggga taataatttt ttagagtgta
    28201 ttcatcaatt ccctccagtt caaaagagct gaaacccggt gtgtcaactg catatccccc
    28261 aaagcttaat ttgaaaattt cagcatgtct tgtggtatgc cttcctcttt ttaatttttc
    28321 actgatttct ccggtttcca aataaaaacc cggctctatg gcattcatca gggatgattt
    28381 tcccactccc gaaggtcctg aaaacacatt caatttgttc ttaagtatgt ttttcagctc
    28441 ttgcagggaa tccgcattat acttgctggt aaaaattatt ttgtaacctg tattcttaaa
    28501 tgtgccaagt atatattcct tttggccttc ttttgctaaa tccatcttat ttatgcagat
    28561 tacagggtct aaattgtttg actttgcggc aatcaaaagt ttatccaaaa gtaaaaaact
    28621 cggttcaggg ttaataacag agaaaattat cacaagctgc tgggcattgg caacaggagg
    28681 tcttttgatt tcactttttc tctccataat ttcttcaata tagcctgcat ctttgtcctc
    28741 ttccgtaatt ctgacaagga ctttatcacc tgccaaaggt ttgatattct taagcctgaa
    28801 caagcctctt gctctgcatt caattgtcct gcttcctata tcaacataat aatttccccc
    28861 aacgcccttt attatgattc cctcaatcat taaaactcct ttttatcaaa atttaatctc
    28921 aactctgtca tatagttcat tgtcaatata tatttcataa attcctgttc cggtcccttt
    28981 aacatttact attatagaac cttcggaagt gcctacctcc tctgagtata caacctcccg
    29041 cccgtccagg gcaattctct ggataacaat tgaaacctta tccctgtctt ggggaagaga
    29101 tatggcaagc ggaaatactc cttcctccgg tataggttcg acaggttctt cggttttagg
    29161 ctttcctgtg cttacagtaa gccatattga tgtaccttgt tccacatcat cgcctgccgg
    29221 gacgctttgt ctcataacca ggccttcagg tatttcctcg ctggtctctt catttacctg
    29281 ccctacagca aggccgtgct ggacaatcga aagctgagcg gtttccaaat ttaaatttaa
    29341 tacatttggc attttcacat atattatttt ctgacctatg cttataacat agtttacttt
    29401 atcattttct tttgctggtg tatttgcagc cgggtactgt tcaataatct gtccggccag
    29461 gaaagtgtca gaattttctt ctgttgcttc tccttccaaa agtccttcct ccccaaggat
    29521 gattcctgct tcaatggcat acctgcccac taaattggga acaagtattt ctttttcccc
    29581 aatgctgata acaacttcta cgggatagtt ttcctttacc tttgaacctt cagcaacact
    29641 ttgttccatt acacggcctt catcgtattc attagaatat tctctctttg ataccttgaa
    29701 ttccaggcct aaatcctcaa taagtttctg ggcttcatct tctcttcttc ccaacaagtc
    29761 gggaaccgga acttcaggca cgtacaagaa tgacctgaat acaattattc ctcctattac
    29821 ggcaataacc aaagcgctta ttattgctgc tatggtaatt tttttgttgt tattgttctt
    29881 ttcatctttt tcgtcttctg cctctccgct aaaaaagctt ttaaaagcac tgtttgaatt
    29941 ctcatcaatc aaatctatat ttattacatt ttcttccaca tcagtaagag caggaatttc
    30001 aatagtagga gaatcgatta tttcaatctc gtcatccttt tcaggttcat tgtttccgtt
    30061 taatgcattc agttttttca gaagttcttc cgtgctttgg aatctgaagc tctgatgttt
    30121 ttccaggcat ttcataataa tatcttcgaa atttttggat attttaatat ttttgccttt
    30181 tttagaaggc gggacaggtt tttcctgaat ctgcttcatg gcaacggaaa tatgattgtc
    30241 tgcatcgaaa ggaagatctc cagttatcat ctcatacatt acaataccca gtgagtaaat
    30301 atcggacttt tcatctacat atcctcctct tgcctgctcg ggcgaaaaat aatgtacaga
    30361 ccctataaca ttggatgtgt tgttgatggt agaacttgtt gaggctcttg caatgccgaa
    30421 atcagttact tttgctgttc cgtcccctgt aataagtata ttgtggggtt tgatgtccct
    30481 gtgtactata ttgtttatgt gagcatgttt caaagcttct gccacctgta ttgaaatttt
    30541 cacggtttcc tgctcactga gtctggtctt tctcttaata tagtttttta atgtttcccc
    30601 cttcacatat tccattacaa tataatatat atcatcttca attccagtgt cgtaaatatt
    30661 aacaatattt ggatgagtga gacttgccgc tgacagggat tcctgcttga atttagctac
    30721 aaattcagaa tcttgagtta attcatccct caaaacctta attgcaacaa agcggttcaa
    30781 tacattgcac tttgctttgt atacatttga catgcccccg ccgccaatct tttcaattat
    30841 ttcgtacctg tttcctagta ttctacctat cataagtttc acctctaatt atattatcaa
    30901 caataattat ggtaatatta tcagttcccc cattatcgtt tgcttgttta atcaatttct
    30961 gcacgctttc agacgtgccg tattccttta taatttccag cattctgtca tcggatacat
    31021 gagttgtgag tccgtctgta caaagaagaa gtatgtcatt tttatacaat tctaattcga
    31081 aaatatcggc atgtacataa tcttcactgc ctaatgccct tgttattaca tttcttttag
    31141 gatgtgtttt ggcttcttct tgtgtaatct ttccatcctt catcagttca ttcacataag
    31201 tatggtctgt ggttatctgt ttaatttcat tgtttcttaa caaataagct ctgctgtcgc
    31261 ctatattacc aatatatata atatcattat tcttatccaa tactgccatt gttacagtgg
    31321 tgcccatccc gcagcattct tcattactga acgacttttc cctgattttg tcattgctta
    31381 tttttacact ttctattatg aacgaaggcg gttgaaaatc ttcatttcca acactttcaa
    31441 cgaataagtt tcttatctcc tcaatagcaa tggaacttgc cacttcgccg gccttatggc
    31501 ctcccattcc gtcagccacg gcatagagac aataattatc aaattgttcc actattaaac
    31561 tatcttcgtt attttcccgc attaagccct tgtttgtttc aaaaaacaca gtcatactca
    31621 tacagcacct cacattttta acaaatcatt tctcagctgg ccgcaggcag catttatatc
    31681 actgcccaat tctcttctga tagttgcatt aatcccgtta tccgccaaaa cttttttaaa
    31741 tttcataatg tctgcatcag ctgacggctt gatgcctgct tcttttatgt aattgcatgg
    31801 aatcaaattt atattagcca gtttattttt caataatctg cttaaatttt cagcatcctc
    31861 ccggctgtca ttaaaaccca ttatcattat atattcaaag gtaagtcttc ttcccgtagt
    31921 ttttaaataa taatcgcagg cattgagtat gtcggtaacc ttatattttt tagctatggg
    31981 cattatctgc tctcttttct cctgggtagg ggcatgaaga gaaaccgcca atgttatagg
    32041 gagattgaca tcagctaacc tgtagatgtt cggcactatg ccgcttgttg aaagagtgat
    32101 ttttcgcatg gatatgtttt gacctctttc atcatttatt atatttagaa atctcactac
    32161 attgtcgaaa ttttcaagag gctcgccgga ccccataatt accacatttg atattttttc
    32221 attagtatct ttttgaatca aatatatttg cttaagcatt tctgctgcag acaggtttct
    32281 tgtaaagccc cttttagtgg aagcacaaaa atgacaaccc atcttgcatc ccacctgtga
    32341 tgagatgcat gcggtattgc cgaatttata ttttaagaaa acactttcta taatgtttcc
    32401 atcttctaac ttaattacat atttttttgt ttcatccaat tttgattcaa gcttaagctc
    32461 aattacagca tgggtaaatt catattcttt acttagttct gccctggttt tttgtgatat
    32521 atttgtcatt tcatcaaagc tgtctacagt ttttttatgt atccagtcaa atatttgacc
    32581 tgctttaaat ttcggctcat taattataaa catttcatct ttcaggtctt caaagcttaa
    32641 atcatcaatt tttctcatat ttttccttct gtatgtgaca ttgaatgttt gctgtcatct
    32701 ttggcacatt gtcactgctg ttctgtgaat catgttttgc gaatcctgtt tcacattgcc
    32761 gatctcaccg tatcaaaata acatatcaaa ttttgtcaat tatatcatat cctttttatt
    32821 ttcgcaataa aaaacccgtc cataccgtga aggtggggat atatttcaag gtaaccattt
    32881 ttggccgttt taaaaaaccc gtctatattt tccgatatat cagccagttt ataatctttg
    32941 ttattattca agaacctctt gataagttcg ttgttttcct caatgtttac agtgcaggtg
    33001 gaatatacca gttccccacc gtgttttaaa tactttgagc cgttctcaag tattttatac
    33061 tgaatttttg ttatttcgtt tacatctgtc ttgttatact ttatttccgg ttttcgtctt
    33121 attatgccaa gccctgtaca tggaacatcc gcaataacgt aatctgcttt tccaatcaag
    33181 gaatcatcca gttttgctgc gtcatatttt tcaaccttga cattatttac acctagtctt
    33241 tttaattcat tttctataag cttaagcttt gccgggtata tgtctcttga taatattttc
    33301 cctgtattgt tcataatttc cgctgcattt aaggtcttgc cacccggggc agcacacata
    33361 tcaattataa atgagttttc ctcaggcttt aaaatttgcc cggctaacat tgagctctcg
    33421 ctttgaatgg aaaaaagacc ctttttatat tcatccgtct tatgtatttc aaaagggttt
    33481 tctacaatta accctttatt ggcatatttg catttgtgag ttagtatgcc tttttcttta
    33541 aagagttctg ccagttcatc ccttttgatt ttaagtgtat taacccttat ttccagtgta
    33601 gcttctctat tgttggcagt taaaacctct tctatgtcct tgccgaactg gtgtttccat
    33661 atcttgacca gttcttttgg atatgaatat ttaacactta aatatccatc ttcaggtaac
    33721 ttactcatct tttccataag agaatccttg cttcgcaata tgtttcttaa aacagcatta
    33781 acgaattttg aagcactaat attgcccttg tcctttatat actggacact ttcatttact
    33841 actatatttt catgggacat atccaaaaag aatatctgat atacagctaa cctcaacaca
    33901 agcaaaacat cagtttccat tttctttgtc ctggtttttg acacttcatc tataatccag
    33961 tctataaata ttaagttctc tataaccccc agaactgatt tacggtataa ggaatgaaac
    34021 ctgtggtcaa catgtttaat gtcattgtta atcacaaggt ttgaatagga cctgtccgta
    34081 aatattttct tcaatgtttc aattgcctgc gccctgtaac tgttcatact tccctcaata
    34141 tgacaaggga cggtggtgcc gtcatcttgt caatctgcct atttccttct taccacttct
    34201 atccaataac catccgggtc aacgataaag tatataccca ttatttcgtt tatgtattct
    34261 atacagccca tttctttatg ttttttgaaa gcttcgtcat aatcgtccgc agccattgca
    34321 agatgaaatt cttggtcgcc caaatcgtaa ggctgtgttc ttccgttcat ccaagtaagt
    34381 tccaatgaaa agtctgtttc tccgtcacct aaatatacca gtatgaaact gccgtcttct
    34441 gcttcttttc tctttacttc ctttaaacct aaagcttctt cgtaaaactt catgctcttg
    34501 cctaaatcaa gaacgttaaa attaaaatga ttaaatgtaa atttcataat tacacctctc
    34561 ttagcgtttt tctatatttt aataaagtat ttttcataag tcaaacttat taattgattt
    34621 ttcttttctc cttaaatgtt aatattaaat tctttagcta tacttccttg tttgtacgga
    34681 tttattttct tgattattcc atcatgtttg aaaagtgatc ctgtaccttt aaaccagaat
    34741 gtaatgccaa attcctcaca ttgcttacga atatccaaca cccagtcata atcacaaagc
    34801 cgtgcttccc gaccgctttc tccattgact gtaacatgat gaataccctt aagataagaa
    34861 gacagattta caggacctaa tagaggtgag caagccacga accgcctttt gacaggcaga
    34921 gataaaaaca agggcaggcg aaggtcggcc atagcctggt tttccactga gcatccaata
    34981 tatacattat catacccgtc accccagtca ccaggaagcg atacgtaaaa acgctcaatc
    35041 cgcttggtta aaatcataaa atcaaggtct gggcgttcct taatcattgt ccaggcatca
    35101 tttcgccatt catctgcttc aggtataaaa aaatcagatg caaagcaggt ggccacagtt
    35161 tttccgcttt ggattttata tttattttta gctgttttta atataggttt atcaaaatca
    35221 gtagttttca ccactgtatt ttgaccacat ctcttagaat acggtccata aaagtaacag
    35281 tatttacagc cttcacttac gtaatagcat ccggtccaag gttcccagtt catagtattg
    35341 cttctccatt tcttagggtt ttatcattat aaaaaaggtg aattttttcc ttctacacat
    35401 attattctaa aattatttaa cagaattatt atcttcaagg atttgactct gaatccatta
    35461 cactccatat aggttttccg ctatcatcaa agctgcatat gaagatggtt cctgtctttt
    35521 tcccaacaga ataatacgag cctccccact cggctgtact ttttacccgg aaggtccaag
    35581 agggttcgtc tatagtataa attgaatcaa ggagtgcttc tgcaaaaaca gcatccttgt
    35641 cttctgtatt tatcggccaa tccccaagaa tattccagct aacaggttcc agctccactt
    35701 taagctttga ctgccccata tcctgtgctt cttcctcagt tatggcatcc tgtgaagtat
    35761 tggcggataa aaactcatcc acaacattat atcttatatc aataccgctg cctccgcttt
    35821 cccaaatact ttcaaggttt accgcatccg gcctgccggt tggatttgaa tgtacgacca
    35881 aacgatatcc accctctatt ttataaacca tgatgtatcg ctcaaaattc gaactgacat
    35941 ttccgccttt atattgccat aaatcctcaa acagcaaatc atcaccttta gcagcaagga
    36001 gacgcacatc ttcaagtgtc atttccttcc cgtccatgtt gccgccccat ggaaatgtaa
    36061 aagaagatgc ttccgtttta ggttcatttt cgtcagtggt ggaattatcc ggctcatcgc
    36121 tgattatttt cactccaaag cacccactca ggactatagc tgccgctatc gcaatgatga
    36181 ttatcaaggg catttttttg aaattattca tagatataat tcacctccgc gattaaagac
    36241 catttttatt tattccccgt tatggtatga taaataattc cgtttaatat ctcagaaatt
    36301 ccataagtcc ttaaattgca ttaattatga gttttgttgt tttgactttc gcttctctat
    36361 taataatttt tctttgttag gtcttgtatc atccaataaa aaatccgccc actcaagttt
    36421 gagtttaccg gcattatcct gccaatacaa cagacggtta taatagtaat taatcttatc
    36481 tattaccggt ttttcaaagt tatccctgcc gaattttaaa actaatgctg ttaactcata
    36541 cataggcaaa ccaattgcat gggtttccac atggacagtt gctccggcat gaccaattgc
    36601 atgacacaaa gcaccatatt ggctgtcatt gatttccttt gccacagcat gagaatctaa
    36661 aatagcccgc tttgcagccg gcatttttat tttccctttt gcccaatcct tacaaagctc
    36721 caaacaaatt cttggtctgt gctcatcagg atatttcgct tcgaattgct ccaaagtcaa
    36781 ttttgaacaa tccaatgccc acattacaag agtacggtga ttttgctttt gaattaaact
    36841 tattaaatcc tgtaaacatt cactgtcacg agaaaataat attttatttc ttttcttcat
    36901 tttaatttct acatccgaaa acatatgttg cttcctccag attgcaatcc cacttgattt
    36961 tatccaaccc ttgtaccatt aggaaccggc ttctcgggat gtgctaaaac aggaataatt
    37021 ccgtcagcat atccaatgtc aaaaagcatt ccttctgaaa tttcacctgc cattttcttt
    37081 ggggctaaat taactacaaa caatgtctgc tgcccttcaa tatccttagg attatctctt
    37141 tcctttttca ttccaaccag aatgtttctt gtaaattcgc caaaatcaac tgttaattta
    37201 accaatttat ctgattttgc aacatcctca actgttctta ttgttcctac cctgatatca
    37261 attttgtcaa gtaagtcaaa agtaatattt tcttttacag gaccagccat acttttacct
    37321 cccgatattc atagcaaagg gacatgaaac aacacatttc ccgcatacat cgcagacccc
    37381 tatatgagaa aactcatctg catttttaag aagaaggtcg tagcatttaa atctgtcaaa
    37441 tccatctgca cttagggcat tttgcgggca tgattctaca cattttaaac aactgccatt
    37501 tttataatac aagcagtatt caatattttc ttctttttct atcggaagct ctgcggctat
    37561 aatcactgtt ccgaatcttc cggcacagcc ttcttttgtt ataatcattc tgtttagtcc
    37621 gaacctgccc atatttgcta tatgcgccgc acttctgtga gaccatgctg atttcaaagt
    37681 attttcatca aaattatgtg ttacttttat agttgctgca tcaatatttt tgttttttaa
    37741 ataatcagct aacttttcag aaatttgatt gattagactg ttagcttcca catagctttt
    37801 agcccattca tatgaaacac cggagccttt aatatttgat ttgacaacgg tttcagaaaa
    37861 tggaataaaa aatgacacaa cacttttggc attgggaaga atatcattgg ggtgcaaatg
    37921 ttccggtcct actatgtcct ttagctggtc atacaaagga ttatcggcag atgaaaaacc
    37981 aacaatgggt ttgcggaaca agcctttgct ttctgattca tgtaccgagt ttttaactaa
    38041 ttcgattatt tcttctttta aactcattgt ctaaatcaac tcctttacat tttaattttc
    38101 atttatcatt ctatcttaac aagctcagct cttataacat ttcatacaac tttaataaac
    38161 tataatttta ttataaatta ttctatattt attatcaaca aaaacaaatc ttctcaatcc
    38221 aatgaataca actaaataat tctgaacttg ttcttaatac ggtttactgt tcaatcacat
    38281 agaattaaac ggcaggtatt aaacctgccg ccaaatataa cctttattta attatgctct
    38341 ttttttcttt aataacatta tgccgcctgc aagcactaat gcacttagac caagaatagt
    38401 gaatgttgat atgctgttat tttcaacttc ttcaaccggt gcgcttatta tctttgcatc
    38461 ttcaacaaca tcctcctcag ccggtgcatt tatatcatca gcttcacctg ttacagtaat
    38521 tatttgtgaa tcatctgcca actcagccgg atctattgag tccgggctgt caggattgta
    38581 ttctaatgtt acagcttgaa taccttcaac tacccttaca ttgtcttttc ctaatgtttt
    38641 aataaccgca tcagcatttt ccggtgtata tggtgtaatt cctacctcaa cataaccatc
    38701 aactgtgctt gtatgagtga caaatatacc cttttcactc aaatcaccgg catagttttg
    38761 ctcaaataac aatttgtcaa tttcagcttg tgcttcatac acagcatcat caactgcagt
    38821 tatttgtatt gatacaggct catctaaatt ttcagccgtc tctgcaaaaa caaaccctat
    38881 tgatgtcacc gataaagcca agctcaatgc tagtattttt attacttttc ttttcatcat
    38941 aataacctct ttctttaaat ctttttgaag ctaaccatgg ccaatggaat aatttttact
    39001 tcttacatat tagacgttaa ttttataaat ttgtttcatg gtaatttttg ataatatatc
    39061 aaattcatta ttatgaaaca actgatgcta tcttgcagta acagatatgg aaaaaattaa
    39121 taagatgaag taaaatggat gcgatatgca gccatttttc tattgcaacg ggttaaatta
    39181 taaattccgt ataaatctag cttcttacaa ggaaatttgg aatcagataa aaaaacagaa
    39241 gtcagtgctt ctgttttttt ataagtattt agtttgttat gatggttata ggtccatcgt
    39301 cttcatcctt gggtttgatt tccacaccgg ctgtttttgt tttctcttta gaaattatta
    39361 tttcttgtcc cgggtaaatt aagtccggat ttgcgatgta gtttgatttt aaaatatcct
    39421 caatggttat attgtgattt tttgcgattt tgtagagaga atctccactt tttacagtat
    39481 aaatcataca atctcccctc tataatttaa taattcattc tatgcagctt cacaataatt
    39541 gttaatttct tattacattt ctaattatta ttttattaca tccaaataaa tagtattcct
    39601 cagccatgta ctccattctt tatagtcatt aaagtaaaag tatttgtcta tttattttta
    39661 atatccctca catgctcctt cacgttcgat aaattataac tatatctctc atattaatca
    39721 tatttactta ttgaataaac atttgctctt tgttacacat aaagccgcat tacacgacta
    39781 tatgactttt tctatcttaa aaatagcata agtcggggtg ttgatgaatc cgtagtatct
    39841 ttcgggattg ttccccagcc aacttttggg tggtaaaggt tctaataatt ctttaatttg
    39901 aaaacccgcc ctgataatag aatttatata atctcccatt gtatggtgat aactaaatga
    39961 caggaagttg ttaacattag gattgtattc aatccatggc tgaacatatc ccctatccct
    40021 gctatccaaa tatctaattg tccattcatc atcatccggc aattctctgt tttcatgtaa
    40081 aattgggtaa tatgctgaat ataccggatg tattacggac aatatacatt caccgccgga
    40141 tgttaaaatc ctgttgattt gctgcattat attgttcaaa ttctttatat aatgcagagt
    40201 tgtcgatgaa aaaacaaaat ctatattttc aagtttcata tctgataaat cttctatatt
    40261 gccatgtaca aactttacga tagatttttt tcttttggca ttattcaaac caatttcaat
    40321 catcttttca gataaatcta tccctgtaat acttctaggg ttcatgctct caaataaaaa
    40381 actaaacctt cctgtgccgc acccaaggtc aactatgctt ttattcttta attcaggtaa
    40441 gattgttttt atcgccttcc attctattaa attactatag gactcttcgt ggtccgtgaa
    40501 taattcatat tcatcagcca ttctgttcca gttatcttta atatctaaat tctccatagt
    40561 ccccttccga tgttcaaaac ccattttttg caggatgtgt ttgcttctat aatttctatt
    40621 tgaatttctt caaattcctg tattcagcat ttaaataatt ttcttattta ttcccaataa
    40681 caagagctat gactattaca ataagcacaa ttgtcacaat aagccagaca ggtacctttt
    40741 tcattaatag aatagttttt ttgacattca ttttatactt ccttaataga tattattccg
    40801 tcccgacctc atgacagtac ccctgtctcc ttgttattac aggatggtat tttcggggaa
    40861 tttatttcct ctcagatagg cttctaccgt cattttattc ttgccgggca tttggatttc
    40921 tttaagtatt actattccgt cttttgcttt aacaaaaatc ccttccttac ctgcttttat
    40981 tatatagccc ggcacatagt ctgtactgtc atttatgtat gatgatttat atactttaat
    41041 agttttttca tccatcgtaa aatatgctgc aggccatgga gacagtcccc ttattagatt
    41101 atggatattc tttgcaggta aactccagtt tattttacgg ttctcgttat caagcatagg
    41161 agcataggtt gaaagtgaat catcttgttt ttgtggtaca atttcatttt tctccaattt
    41221 aaccaaggtt tctatcaaga ggtccgcacc tttatgcatc aaaatataat gaaggtctcc
    41281 aacattcatt tcttcatcaa tttccacttc tgcttttaaa agcatgtcgc ctgtatctaa
    41341 tccggtatcc atctgcattg ttgttacgcc tgttttgtat tccccgttta ttattgccca
    41401 gttcaagggg gctgctcccc ttaatttagg cagcagggat gcatggacgt tgatgcatcc
    41461 gtatttaggc aatgtcaaaa tttccctgct cagtatttgt ccataagcaa ccacgactat
    41521 tgcatcagga ttgtatgatt ttaatacatc aatgttttcc tgcttcttta ttttatcagg
    41581 ctggaaaatt tcaagtccca gcttttcagc tgtttctttc acttctgact ttttcagctt
    41641 tttacccctg ccaaatggtt tatcaggctg ggtaacaacc aaaaccacat tgtgaccgct
    41701 ctcataaagt ttttccaggc ttggaactgc aaagccagga gtgcccataa atattatatt
    41761 catattatca ccttactctt cgcttgtgtc ttcaattatc ctgtctacaa acaacactcc
    41821 gtctaaatga tcaatttcat ggcataaagc ccgtgccagc aaaccttccg cttctatttt
    41881 ttttgtttca ttattttcat ttgtatattc tacagttacc ttcattggtc ttgacactgt
    41941 gccctttttc tcaggaacac tcaggcatgc ctcctgggct gtctgctctc ctgaatcctt
    42001 gataatttta gggttggcca ttttataaac cttgccgtct tccacgtcta ttacaatcac
    42061 acgcttaagt atccctacct ggggagcggc aagtccaaca ccttcaaatt tatacatggt
    42121 ttccaccatg tcatccataa gcatcctgat tctatcatca acaagaggaa cttccctgct
    42181 tttctttctt aataattcat cgccttcgta tcttaaattt cttaatgcca tttatatcct
    42241 cctaaattgt atccgtatta atatttatgc ttacctttat attttctata tccttatcca
    42301 tatatacagt ccttagtatt tttttcagtt ccggcaagct gtgatttgac accttgataa
    42361 ataaattaat tctgtactca tcatttacct tgtagatatt gtggggtaca ggcctgtaca
    42421 ataataagct tctttccttt atcatttctt gtacttcttc ttttaccagg gaatattttt
    42481 cacctgctgt ttttgcaacc agctcttcat tctttgatat taagcatata tttattatat
    42541 taataaaggg cggaaatgca aattcttttc ttagttttaa ttcctgctgc aaaaataaat
    42601 catagtcatt gtttcttgct gcattaatga tgaaattttc aggctcataa gtttgaatgt
    42661 acacattgcc tttcttgtct cccctgccgg cccttccgct gacctgggtt atcagctgaa
    42721 atgttttttc gcttgcagtg taaaagggta aattcagtat tgcgtctgca gaaagtactc
    42781 ctactgtagt tacattggga aagtcaaatc cctttgcaag catctgggtt cctatgagta
    42841 tgtcaatttt cccgctttta aaatcattat agatgttttc ataagcttct gagtcagtca
    42901 ttgagtcaag gtccattctt tcaattgatg tatggggaaa taattgattt atcaattttt
    42961 caacatgctg ggttccggca ctgaactgct ctattttatc acttttgcat tttggacatt
    43021 cataaatcat ttttcttgtt cttccgcagt agtgacattt catgatattt ccctttatat
    43081 ggtaggtcat ggaaacgtcg caccgctggc accttacaac ataaccgcat tttttgcagg
    43141 acacgaaacc ggaatagcct cttttattga gaaacaatat tacctgctca ttctgcttaa
    43201 gggaattcct aatgctttgg tataattcct ggcttataat ggtattgttt cccttgtcaa
    43261 gctcatctga catgtttata atcttcattt caggcataga ggcgtcttta accctgtttt
    43321 taagttcgat taattcaatc tgccctttca tagccatata gtaggtgttt aaggacggag
    43381 tcgcagaccc caatataacc ttgcctcccg ccatatatgc catctttact gcaacctcca
    43441 tggtgtcata ctttggagat gttgctgaaa tatagctgtc ctcatgctct tcgtctatta
    43501 ctattgcacc caaatcactg attggggcaa atatggcaga cctggctcct attacaatat
    43561 ccgcctcttt gttatatacc cggagccact caatgtacct gtcttttttg cttaatttag
    43621 aatgaaatac tgcaatcttg tttccgaatc tttccctaaa tctgtcaatt gtctgggctg
    43681 taagggaaat ctcgggaaca agcataattg actgtctgcc ttctttaata tatccttcga
    43741 ttatattcat atacacttct gtcttgccgc tgcctgttac tccgtggagg agaaatgctt
    43801 ttttgtctga acgaagaatt gtttcacagg ctctcttttg ctcttcattt aagcataagg
    43861 gctctttagg ctcaaactct tcctgctttg aataatctaa aaccggtttt tcctctaata
    43921 catccttttt tatcaaatta tttataactg catatgaaac atttaatttc tctcttagct
    43981 ctgcaatgct gactgattga tgctgtttta gatactctat tacctttatc tggttaggag
    44041 tgtaattaat cttgtacttt tcggtttccc tatattcctt cagacttgca tgcttttcgt
    44101 aaacgctttg agtatcggaa ggtatcatca atctcattgc atcggagtat ttgcatatat
    44161 acctttccct tatccaaaag ataattttaa tcatttcttt tgttactata ggtttctcat
    44221 ctattatttc aataagaggc ttgatttttt ccacaggata atcgcaggct gaatcaaggg
    44281 aaactattaa tgcattgata atacttttcc ctctgccaaa atttactctg gccctttttc
    44341 cttcctcagc tgagtttaat agtgcatccg gcactccgta cgtgtaaagc tgatctgtgc
    44401 ttttggaaat attgtctaaa attacctgtg caaatctatt atacatatct tccccgctca
    44461 attaaaatta aataatcagt catttgactg attatctgac ttttcttgtg ccgttgttt
//
LOCUS       DAOPUT010000004        39347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_962_length_39347_cov_19.449710, whole genome shotgun sequence.
ACCESSION   DAOPUT010000004 DAOPUT010000000
VERSION     DAOPUT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 39347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39347
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_962_length_39347_cov_19.449710"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..2282
                     /gene="glgB"
                     /locus_tag="PKK75_00985"
     CDS             363..2282
                     /gene="glgB"
                     /locus_tag="PKK75_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNZ82198.1"
                     /translation="MKTNESLPVYLFHQGTNYYSFNFLGSHIIENAGTVFRVWAPHAK
                     QVHLVGNFCSWQKNPGYEMKKIPESGIWELFAPDISEGELYKYIITAADNALIYKSDP
                     FAFSSQTKMETASIVYNIDKYNWNDINWLEYKKTVNIYELPVNIYEVNLSSWKRKENG
                     DYYSYKELAPLLIDHVKKHFFTHIELMPVMEYPFDGSWGYQVSGYYAPTKRFGEPDDL
                     KYLIDQCHQNGIGVVLDWVPAHFPKDEHGLYKFDGEYLYEYKDSQKREIGSWGTHKFD
                     FGKTEVQSFLISNAVFWLDVYHADGLRVDAVSSMLYLDYDKKEGEWTPNVYGGNENLE
                     SIAFLKKLNEAIFRNYPNTMMIAEESTAWPMVTKPTYLGGLGFNFKWNMGWMNDMMEY
                     METDPFFRKYKHKNITFSLHYAFSENYILPLSHDEVVHGKKSLLNKMPGPYEEKFANV
                     RVLLGYMIAHPGKKLNFMGYEVGQFVEWDFNKEIEWFMTDYEYHGKLNTYIKELNEFY
                     LKQPELWEVDYSWNGFKWISNDDFDQNIIAFKRIDKNNNELIAVVNFSPAERQNYLIG
                     ADRGIYTEIFNSNCEEFGGTGSGNKDKIVSSQGSLHGHESYLSLTLPPLSIIFLKKEN
                     KYNINNKGGKIYEIS"
     gene            2269..3429
                     /locus_tag="PKK75_00990"
     CDS             2269..3429
                     /locus_tag="PKK75_00990"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003347219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNZ82199.1"
                     /translation="MKLAKKECVAMLLAGGQGSRLGALTTKIAKPSVPFGGKYRIIDF
                     ALSNCVNSGIDTIGVLTQYQPLTLNSYIADGKSWDLDRMYGGVQILPPYQRVADSEWY
                     LGTANSICQNMNFIDQYAPEYVLILSGDHIYKMDYSLMIDYHKKMDADCTISVIDVSL
                     KEASRFGILSSDETGRITDFEEKPKKPKSTQASMGIYVFKWNKLKKYLEIDDKNPDSS
                     NDFGKDVIPLMLEKNEKMYAYKFRGYWKDVGTIDSLWQANMDLLGENGFELSEENWII
                     YSRHAEKPPHYAGRDAVIKNSIVSEGAVILGTVENSIIFSGVRVGENAVVKDSVIMAD
                     VEINDNCVVNYSIIDEMVKIGPGLKIGKPMEENSEITVIPRESIINNMGGII"
     gene            3426..4544
                     /gene="glgD"
                     /locus_tag="PKK75_00995"
     CDS             3426..4544
                     /gene="glgD"
                     /locus_tag="PKK75_00995"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HNZ82200.1"
                     /translation="MNALGIIFSDIQDWSMTELASHRSVAAIPFGGRYRLIDFPLSNM
                     VNSSINKVYLITSSNYRSLMEHIGTGKDWDLSRKKGGILTIPPYAAGENHGLYQGRLD
                     ALKRVLDYITSSKENYVVLSDSDMIYDINYQKVIKYHEEKNSDITAVYTKININKSES
                     IHKILYEFDQDGRADDILVYPITNGIHNVGLNTWVMNKEFLESLLYDAIARNYKDFSL
                     NVLSAKVHSLRIYGYEYTGFFAHIDSLQSYFENSMKLLNIDNLQNIFYSKTGPIYTKT
                     KDSAPTKYSKDAVIKNSLIANGCVIEGTVENSILFRNVRIGKNAVVKNSIIMQHGIIS
                     DNSKLDSIITDTYTFVSKDRVLLGYSNCPFFIKKNSVV"
     gene            4556..5995
                     /locus_tag="PKK75_01000"
     CDS             4556..5995
                     /locus_tag="PKK75_01000"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNZ82201.1"
                     /translation="MSKLKVLYAASEAAPFIATGGLGEVSGSLPKALAEKYKDKIDIR
                     IILPLYQGIEDRSEFEFLGKTSVPLAWRQQYCGVFKRVVENVTYYFIDNEYYFKRSEC
                     YGHFDDAERFAFFSKAVLYILPMIEFMPDIIHANDWQTSLVPIYLNTKYSGYEGYRDI
                     KSIITIHNIEYQGVFDLSISEDVFDLHGKEKSIIEYRGAINLLKGALETAHIISTVSE
                     SYSKEIYDDYYAHGLAEIIQKNSSKVRGILNGIDAEKYNPENDPEIFENYTAKTIKKK
                     YLNKKNLQSLSGLPQDKDVPVIGIISRLVKHKGIDLIINAIEGILKEKVQFIILGKGD
                     RGYELYFKYLQESYHDKIYVKIGFDSSFAKKIYAGSDLFLMPSISEPCGIAQMISSRY
                     GAVPIVRETGGLRDSIKDASLGEGNGFTFSDQTPEALCDAVKRALKVYENKEDWKRLV
                     ETVMNTDFSWKKSAEKYFYMYSSLADIGE"
     gene            5998..8412
                     /locus_tag="PKK75_01005"
     CDS             5998..8412
                     /locus_tag="PKK75_01005"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNZ82202.1"
                     /translation="MRSKIDARQINRLIDEEIIRYWNTEPEAASINQIYRALSFVIRD
                     LLLRKRNKFKRMQNEHKQVYYICMEFLIGKSLKNNLFNLGIEEESREALKKYNITPEE
                     LYAIEKDPGLGNGGLGRLAACFMDSLATLDYPAVGFSIKYEFGLFKQKIIDGWQTELP
                     DDWLTTSDVWLMPRTDERVVVKFDGAIREEWTREGMKVHHEFATEVEAIPYDVMISGY
                     DSKGVSLLRLWESRSLNSIDMNLFSQGDYIRALSEDSMAEAISKVLYPADDHNEGKVL
                     RLKQQYFLVSASAQNIVKYHLNKYNSLDNFSEKNAIHINDTHPALIIPELMRIFMDEY
                     GLSWEKAWNQVVNTVSYTNHTVLKEALETFDESMIQTKLPRIYMIIREINNRFLDEAA
                     MKYENYSKPLNMSIFGYGRVNMANLCIIGSHNVNGVARLHSEILKRTVFKHFYDYTPD
                     KFMNVTNGITHRRWLNQANPSLEKLLDELIGPGYRKQPERLGMFKHFKDDDGVLNRLD
                     EIKYINKRNLVEYVRANNHIELSIDSIFDIQAKRLHEYKRQLLNALRIISIIIDINEG
                     NTRDIVPETFIFAAKAAPGYHRAKEIIQLIYALSEYINKNTFIKKFINVVFLENYSVS
                     LAEIIIPAGEISEQISLAGKEASGTGNMKFMLNGALTIGTYDGANVEIINEVGEENFF
                     LFGLRDYEVEELWKSGYSSVAYYQKNEKLRKIIDFLNRGIAGKPFESISRYLIGGPVV
                     GDPYMCFADFDSYMKVHERASFLYYNDKRKWNQMSLINIASAGVFSSDRTINEYAGKI
                     WGIKPI"
     gene            8541..10310
                     /locus_tag="PKK75_01010"
     CDS             8541..10310
                     /locus_tag="PKK75_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNZ82203.1"
                     /translation="MYKFNSQSEFYRHPQGGIKSNEIITFKIYVKRNSSRPELLIEKR
                     HNHEKSFYGKIKMQWISQEKNYDLYQAEVSIKEYGNYYYSFIFNEETTRDYELLIYDE
                     GYLTPDWTKGGIIYHIFVDRFFRRNIIKKNGAIIRKWGEIPYYLPNTEGEILNNDFFG
                     GNLEGIREKLPYIAKLGVSIIYLSPIFEAYSNHKYDTGNYHKIDPMFGTEHSLKKLIR
                     EAKKYGINIILDGVFSHTGDDSIYFNKYGRYNSIGAYQSKDSPYYNWYTFNNWNHDYI
                     SWWGIKTLPEINENNDSYVDFITGKKGVIHHWQKAGVMGWRLDVADELPDDFISALRS
                     SIKEYNNDALIIGEVWEDASNKYSYDKLKEYFCGRQLDSVTNYPLRDGIIQFIKSRDC
                     TYLHNIMNKIIETYPPQTVNCLMNVLGTHDTERILTVLGSENLPSNKIEMAESKLSGE
                     ELKQGTNLLKIAVLLQMTLPGVPCIYYGDEAGMEGWEDPLNRRCYPWGNENEEILSYY
                     MLVTSFRRKNKVFAEGKYRCLIHDKGVFVYERYDGNEKTIIGVNLSKKEITLKFKENM
                     TEYTNNIKNNVFNIEKEGFLILI"
     gene            complement(10378..11556)
                     /gene="metK"
                     /locus_tag="PKK75_01015"
     CDS             complement(10378..11556)
                     /gene="metK"
                     /locus_tag="PKK75_01015"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNZ82204.1"
                     /translation="MKKWLFTSESVTEGHPDKMCDQISDGILDAIMELDPNGRVACEA
                     ITKTGFVLLAGEISTSCYIDIPQIVRNTIKNIGYDNAEYGFDYKTCAVFTSIEEQSQD
                     IALGVNEADEYKKDHNDANDRIGAGDQGMMFGFACNETPELMPMPISLAHKLSKKLAE
                     VRKNKIVDYLRPDGKTQVTVEYHDNKPVRVDTIVISTQHNPEVDIKTIAEDLIEIVVK
                     PIVPAELLDENTKYYVNPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKD
                     PTKVDRSAAYAARYIAKNVVAAGLADKCEVQVAYAIGVAKPVSIMVETFGTNKVADEI
                     IEKAIAENFDLRPAVIIEKFNLRRPIYQQLAAYGHFGREDLDLPWEKTDKKDVLKKYL
                     "
     assembly_gap    11593..11692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      complement(11717..11812)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            12043..14634
                     /locus_tag="PKK75_01020"
     CDS             12043..14634
                     /locus_tag="PKK75_01020"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNZ82205.1"
                     /translation="MILKKIAAISAAAILLLSSSAYGSSLYTVYDLSEETRLSKSITY
                     ERTEKYTSSGWMNINVIRADLTDDHTEIKPVTNEDGISNRAVLSSMIKSSGAVAAVNG
                     DFFYMGDPTYAYGPIIRDNKLITSPLPFSYGYPTVSKLEDKIDISVWDPKITLYGSDS
                     TEFNVTVVNKTSSLEWGPTILTSDWSNTSPGYTTKDIVEVVIVDNTVSQVRKNQPSTA
                     IPENGYIIASSNAATMDMMENSFREGRPVSLNIELDFDPEDVDWAFGGLNYLVKDGRE
                     NEISSQVSGANPRTAIGFSMDNTEIIMVTIDGRHKDYVGAGQKELAKIMIELGAYNAV
                     NMDGGGSTTMGIDFLRNADVTVVNIPSDGRERRIASGVGVFNNYPDSRRVEIIEIKAE
                     QNKVFNKTETPLELKFFNEYYSPVDYDLKNVDFTVYPAGAGRVANNVFMPERPGKATI
                     TARAGNAEGHIEIDVLDKAQALEVETDSLTLGFNDKYELGQVMGIDKYGNSAYIPWKY
                     IAITYRNKVGKVENGLFTSSDISNTGAVTLTFGDAVKHIQVKTGYRYKTLNRFENLDN
                     LKLTLYPEDSYGYMAISKDFVKEGENALKLDYDFTKMTDQSIAFVEFGSDGQGIKLED
                     KPLAIGMWVYGDNKNHWLRTRITDAYGTQVKIDFEEEVNWTGWKWVEAHIPEGIAYPI
                     TLKNIYLAEINETRKDSGTIYIDNLRILYEPKDKELGLRAETLFEDTMKSSAITNYSE
                     KLTISQGKYKYEGTDSIIHCEGIISNGTMSAADITMWSNIKSFMEFENKVLVLSLNSH
                     IHNIYDKREVRILKEILEKASQKNDVFVVFKGEDENTVIENSVRYITYDDSFEIGVSQ
                     DRVSYKN"
     gene            14745..15170
                     /gene="mscL"
                     /locus_tag="PKK75_01025"
     CDS             14745..15170
                     /gene="mscL"
                     /locus_tag="PKK75_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNZ82206.1"
                     /translation="MWKEFKEFAFKGNVLDLAIGVIMGGAFGKIVTSVVNDLIMPILG
                     YLMAGMDFKSLKFVMSEAIMQGDTVIKPEAAILYGNFIQNIVDFLIVALSIFLFIRLI
                     NKSKEKQKKQEEAVPEPEVPAGPSQEELLTEIRDLLKEK"
     gene            15285..15782
                     /locus_tag="PKK75_01030"
     CDS             15285..15782
                     /locus_tag="PKK75_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNZ82207.1"
                     /translation="MSLYKQWTENLEAIKSQGEYNDFFNAYMKKETECYRNLLENKNP
                     VIEGKIQELAEKNNMTPLEFTGFLDGANTSFKEALVLDDLTGDSEIKAEFDFKKLLWN
                     MYDVKAQWLYGMKEWENIFDEEKRRQIKKEYNRSKIVVKGEKTGRNDPCPCGSGLKYK
                     KCCGK"
     gene            15795..16658
                     /locus_tag="PKK75_01035"
     CDS             15795..16658
                     /locus_tag="PKK75_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ82208.1"
                     /translation="MDKQKGIVFGILAAFIYGFTPILGKLTYLEGSNTMSLAFYRNLF
                     SLPFLFLMLKYNKVELKTDKVQLKKLALLATMGPTLTSLFLYGSYIYITVGMSTTIHY
                     IYPVLVAAASMLIFKEKISKEKLIALLLSTAGAAMFFEGDFNITGVLLAFSSGVIYAA
                     HVLFIDKSGLNEIYPFKITFYLSIFSSIYLFIAGLISGSLVFTITPKGWLFTILVAFF
                     VAFLANTFIPISIKYAGPTVTSIAGLFEPITSIFTGVLFLSEPLTFKSVTACILILLG
                     VLIVTLTKEMP"
     gene            complement(16712..17575)
                     /locus_tag="PKK75_01040"
     CDS             complement(16712..17575)
                     /locus_tag="PKK75_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNZ82209.1"
                     /translation="MSVETVKLTGKPVAEHLRANIKTRVAGLNERHIVPAIIIIRVGN
                     KEDDVFYERSILKNCSLLGIEGRVKELPENVSMEKLGKTIEDANEDDNTHGIMMFRPL
                     PGHLNEKEILEKINPVKDIDGMTPVNLEKIFEGDDSGFSPCTPKAVIEMLKYYQIPLK
                     GANVAVAGRSLVVGKPLAMLLLKENATVTICHSKTKDMRSITSQADIVVAAIGKAKFM
                     NEEYFTESSIVIDVGVNDDGNGKICGDVDYDKVFGKVKMLTPASGGVGTITTTILLEQ
                     AVRACEMMTDK"
     gene            complement(17602..18231)
                     /locus_tag="PKK75_01045"
     CDS             complement(17602..18231)
                     /locus_tag="PKK75_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNZ82210.1"
                     /translation="MLTELSCKDFAEELASKKPVPGGGGAAAMVAALGAALNTMVANF
                     SYGKKKFIDIKDKHEELIKRGEILRVKLIDLVNKDAEVFEPLSRAYVMPSNTDEEKAL
                     KNKTLQRYLIEACSAPMETLEFSYDAILLHEEILDISSKNIISDVGVGVQFLKAALNS
                     AYLNVLINLNSMDDEDYVRGQRDKAEKLVDRGTKVADEVYQKVLTVLNQ"
     gene            complement(18266..19012)
                     /locus_tag="PKK75_01050"
     CDS             complement(18266..19012)
                     /locus_tag="PKK75_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNZ82211.1"
                     /translation="MSSISLTPKETAEALIKAAQNKVSLTVQKRLLMSIMGGMYIALG
                     AQGFIIAFDNMFIRAAVFPVGLMLIVLVGGELFTGDCLMTFALLKGEIKASNYTSTLL
                     QVLAGNFIGGALTAGLLYAGGVYNNPVLAERIIQIAEAKLSLPFMQVVSKGILCNILV
                     SLAVWFSITSKDTTGKILGCWFPVMLFVLCGYEHVVANMFYLPMASFLDTSINIVNMT
                     IYSLIPSALGNYIGGGLLIPFIYNKVYFKD"
     gene            complement(19139..20824)
                     /locus_tag="PKK75_01055"
     CDS             complement(19139..20824)
                     /locus_tag="PKK75_01055"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNZ82212.1"
                     /translation="MPDTFLSDIEIAQQTKIAPINTIAEKLGIPDKYVENYGKYKAKI
                     DYRYLQNELKDNKDAKLILVTAITPTPAGEGKTTTTIGLGDALARLGKNTIITLREPS
                     LGPVFGVKGGAAGGGYAQVVPMEDINLHFTGDFHAVGAANNLLAAMMDNHIHQGNELN
                     IDTRRIVWRRCVDMNDRQLRHIVDGLGGRTEGVVREDGFDITVASEIMAIFCLSNDIM
                     ELKENLSKIIVAYTREGNPVTAGQLKAEGAMAALLKEALKPNLVQTLEGTPAFIHGGP
                     FANIAHGCNSVIATKMASKLSDYTVTEAGFGADLGAEKFIGIKCRKTGLRPNAVVIVA
                     SVRALKHHGEVKKADLGKENLEALDKGLPNLLKHVENVTQKFKIPAVVAINKFPTDTE
                     AELNLIKERCSELGVNVALSEVWGKGGSGGKELAEEVIRLAEKESSMEFPYTDEMSIK
                     EKIEAITTKIYGAESVEYSKKADKEIENLEKLGFGNLPICMAKTQYSLTDDPKVIGRP
                     AGFKIIIRDITISAGAGFIVALTGEIMKMPGLPRVPSAENIDVDIDGKIKGLF"
     gene            21194..22291
                     /gene="gcvT"
                     /locus_tag="PKK75_01060"
     CDS             21194..22291
                     /gene="gcvT"
                     /locus_tag="PKK75_01060"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNZ82213.1"
                     /translation="MTAKKTPLYGEHVKLGGNVVDYAGWFLPVQYTGLIPEHEAVRNA
                     AGIFDVSHMGEITVKGKDAVAFVDYLMTNDITKIVDNQIIYTLMCYPDGGVVDDLLVY
                     RYENNYLYLVVNASNTGKDYAWILEHKGDFDVEIENISDSVGEVALQGPFAQKILQKL
                     TDFDLDKIEFFHLQRDISVDGVNCMISRTGYTGEDGFEIYADNEGIVKVWNSVLEAGK
                     EFGILPAGLGCRDTLRFEASLPLYGHEISQEITPLEGGFKYFVKLDKAADFIGKEALN
                     KQWSEGLKRKLAGFELIGRGIAREGMEVQKDGVIIGHVTTGYMSPTLKKTIGNALLAS
                     EFTELGTEIEVMVRNKPVKAKVISKKFLKKK"
     gene            22323..22706
                     /gene="gcvH"
                     /locus_tag="PKK75_01065"
     CDS             22323..22706
                     /gene="gcvH"
                     /locus_tag="PKK75_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNZ82214.1"
                     /translation="MKVVKGLYYSNDHEWVKVDGDEAYVGIADFAQEHLGDIVYVELP
                     EVDDEFAKEEAFSVVESVKAASDIYLPVSGKVIAVNEALEDEPALLNADPYENWIIKI
                     VLNDKAELDELMTSEDYEKFLAEEE"
     gene            22708..24054
                     /gene="gcvPA"
                     /locus_tag="PKK75_01070"
     CDS             22708..24054
                     /gene="gcvPA"
                     /locus_tag="PKK75_01070"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNZ82215.1"
                     /translation="MYPYIPNTPQDEQEMLKTIGLDSINQLFGDIPEQVHLTKPLNIP
                     DSKSELEVSGYLKNLADKNCPVSQLACFLGAGAYDHYIPSVINAITSRSEFYTSYTPY
                     QPEISQGTLQYIFEYQTLITNLTGMDVANASLYDGGTAITEAALMACSYSRKDEIIVS
                     AGVNPEARQILKTYAHAQNLKVTEVETKDGVTDAGMLESLLSDKTAAVIVQNPNFFGL
                     LEDLKGLAEITHKVKKASFVAAVEPISLALLKKPGELDVDIVVGEGQPLGMPLQFGGP
                     YLGFMAVKNDYIRKIPGRIVGETTDLDGKRSYVLTLSAREQHIRREKATSNICSNQGL
                     NALAATVYMVTMGKEGLKEVAEQCVQKSHYAFNELTKSGKYRPLFDKPFFMEFALTSE
                     DSVDKINKALLEEKIIGGYDLGKDYPQYKNASLYAVTEKRTKEEIDKLRRVLEAVK"
     gene            24051..25511
                     /gene="gcvPB"
                     /locus_tag="PKK75_01075"
     CDS             24051..25511
                     /gene="gcvPB"
                     /locus_tag="PKK75_01075"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNZ82216.1"
                     /translation="MMKEYNKLIFELSKPGRKAYSLPALDVEEKALDSLIPAEFLTDK
                     GLNFPEVSEVDVIRHYTNLSNKNYGLDKGFYPLGSCTMKYNPKINEDVVKYDGFANVH
                     PFQAESCIQGSLQVMYELERSLCEIGGMDSFTLHPAAGAHGEITGIMLIKAYHDKNGD
                     TKRNKIIVPDSAHGTNPATAAMAGYSIVEIKSTENGLVDLEALKAAVGEDTAGLMLTN
                     PNTLGLYDKGVKEISRIVHEAGGLLYYDGANLNAIMGHSRPGDMGFDTVHFNLHKTFS
                     TPHGGGGPGSGPVGCKKILAEFLPKPLVGKRYDGTYFLDYNVPGSIGKIKDFYGHFGI
                     FLRAYTYILIMGNDGLKKASEMAVLNANYIKEKLKDYYYLPIDTLVKHEVVFGGLKDT
                     TSGVTTLDIAKRLLDYGYHPPTVYFPLIIDQALMIEPTESESKETIDGFIEAMIAIAK
                     EASSDPELLKTAPHDTLVRRPDETKAARNPVVKYQG"
     regulatory      25560..25653
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            25719..26459
                     /locus_tag="PKK75_01080"
     CDS             25719..26459
                     /locus_tag="PKK75_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HNZ82217.1"
                     /translation="MSSNTERHKFTSLTIVRAGLFISMSLVLKVLFEVYIPLAGIPAL
                     RINFANVPLMLSGIILGPFAGFLTGAAADIINFIAKPSGPYFPGFTLASGLCGLIPGI
                     IFKYLKRDINYNLLNTIFISLLSVGFVLVFMQNGLLTFDNGIFYDGKPLNAAYAAGFF
                     LLVAIYLIIPAKVSSASKDLAMNKIVFAISATQLITSIILNTYFLYVLYGIGIMITLP
                     ARILTNFFMIPLYSIIIGAILNAVKGRI"
     gene            complement(26528..27745)
                     /locus_tag="PKK75_01085"
     CDS             complement(26528..27745)
                     /locus_tag="PKK75_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ82218.1"
                     /translation="MIYDVIVIGAGPAGLFSAANIENKKVLIIEKSKEPGRKLLLTGA
                     GQCNYTNNCAINEFFDRYGDKGRFIKPALYNFTNQETIDFFKKRGLRSIIREDDKVFP
                     SSLKAIDVLNVLIDSVNHAQMLLNTSVERVSYDADKKLFYIKTHAFAYAAYNLIIATG
                     GKSYPDTGSTGAGYEFAASLGHSVVEPKPALTPIYVENYQFGELSGVSFKNIKLSLFK
                     NNKKTKEFAGDFLFTHINISGPVIINNSRYMEAGDIIKINFTAFSNEDDFRSFFESLI
                     QSKTSIKTALKDLALPRRFVEKALEICGIDENLPCSQLSKIQRNSLIQMFSAHPMKII
                     KLGDYNIAMVTKGGVSTSEVNSKTMESKKIPGLYFTGEVLDYDGDTGGFNIQAAFSTA
                     KLAADAINKHLKD"
     gene            complement(27745..28686)
                     /locus_tag="PKK75_01090"
     CDS             complement(27745..28686)
                     /locus_tag="PKK75_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82219.1"
                     /translation="MRKFRLYLIVFTLLFAMGCTQIPVSQETGLGPEEYEGIAGTEEI
                     KKENLSLKEELDGTKAELNEFKTKFEELEDEYLSLAKSNENIIFKLEEAESRLKIVQS
                     GDMPKFNSENTDADSIVSYLKESSELVDDSVKGIEIIGTDENIVFRTQGYGDDYSQIF
                     IWDEGENQPAAIDGATFDREGSYEWLGSYILIKNSDGKNKILDIENKKIIGSFDEPQK
                     MQLLEGTTTILLKDKDSKFVLFDFVNDSNKQINLDNNKYTDFNLENDSVVFTGAYVEN
                     EVEYEIKAAFALDKMMETYEIPKAGEIIETEEMEDTV"
     gene            complement(28735..30573)
                     /locus_tag="PKK75_01095"
     CDS             complement(28735..30573)
                     /locus_tag="PKK75_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ82220.1"
                     /translation="MKKISRIILLSVVLSLLFTMTANAIVFSDISNHWARSYIERVEK
                     DGLVSGYEDGTFKPDNNVTVLESLIMLSRLYTIDDDIKKEIIDEYKPSLGRMQNVLYN
                     EWSLDYLAVAIGLGIVSEQAVKDMFANKNIFQDAKREEVAVLLTKALGLEDEAKSLKT
                     YVLPFKDNAEISASARPYIYLMSEKKIILGDNEGNINPVDEIKRAEIATLLVKAFDYI
                     SDNNILPDLDKYQPATKVSGIITEVETKRTDSYIYVKDDRGAESKVSVNDDTKITING
                     RTRELSDLKNDMLVDCRVNEEGLALEIEADSTKDVVRGTILYVAYVEPASITIYDEDD
                     DKLTFEIEADVDVYHDGKATELRRLEKSDEVTLLLDDGQVYRINSVSRIKYFDGEITA
                     IDYNYPIKVTVKTDEGLLKTFIFTSSVDVTKNDDDSSFDQVRVGDEVTITTEYDDMIA
                     INTIAKEAEASGVIKEILIAPQSKIKIADEDGAIKQYSVSSNVKITIGTKNVSIYDLR
                     LGYNAYINTSGDEIVSMEVSELDTAKSFIGKIIFINEDDRLIMMQSITGTGKTELIYL
                     TVANRTIIYDTSGETKYFKDLEEGESIVAIAVQRGGEYEATSIMIQ"
     gene            30759..30893
                     /locus_tag="PKK75_01100"
     CDS             30759..30893
                     /locus_tag="PKK75_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82221.1"
                     /translation="MAGVNYSRFKLKNSIFMNIAINEFIKRAAENFYIIKFEGEIWAY
                     "
     gene            30881..32128
                     /gene="aroA"
                     /locus_tag="PKK75_01105"
     CDS             30881..32128
                     /gene="aroA"
                     /locus_tag="PKK75_01105"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNZ82222.1"
                     /translation="MGILIKPSLLRGKITIPFSKSLSHRAIICASLSKEESKIKNIVF
                     SDDIRAVIEGMKKLGADIREADDFLTVKRAENTGDNAVNNIIDCKESGSVLRFLIPLS
                     LVFKDKCTFTGKGRLVERPLDVYYDIFEKSNIEYETNKGKLPLTVKGSLKGGYYSIPG
                     NISSQFITGLFFALPLLKDNSEIRITGPVESKAYIELTRDVMKKYCVNIEKIDSCNYF
                     IKGGQKYKAHNCEIEGDFSQAAFFLAANALGSKVECLGLNYDSLQGDREILNIIEKYS
                     LPENEITIDASQIPDLVPVISVLASLKENCKTTIINAGRLRYKESDRLKAIAAEMKKL
                     GASIKEIENGLIIEGKNSLHGNTTVYSWNDHRIAMALAIAAIKCDDPILLEGYESVKK
                     SYPKFWDDYKSLGGVINELNDRK"
     gene            32106..33215
                     /gene="aroC"
                     /locus_tag="PKK75_01110"
     CDS             32106..33215
                     /gene="aroC"
                     /locus_tag="PKK75_01110"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNZ82223.1"
                     /translation="MSSMTGNKLKISIFGESHGEGIGVVIDGLPSGFALDFDYINQQM
                     NRRLPGTNTLSTERKEKDEYKILSGLFEGKTTGTPLSAIIYNIDKKSDDYGERFKLRP
                     GHSDYTGYIKYSSYNDYRGGGHFSGRLTAPLLFAGALAMQILESKGILIGSRIKSIYN
                     INDDDIENMDADTIKNLRSMSFPVISKEKGALMTKNILEQKEKGNSLGGIVETFIINV
                     PAGYGEPFFDSLESKMAHMIFSIPGVKGIEFGAGFDITTLTGKEANDEFYVANHKIIT
                     VTNNNGGVLGGITNGMPIVIKTAIKPTPSIWQPQNTVDISSMENTVVNIKGRHDPCIV
                     PRVVAVLEGACALVLLDLIMERDGENWNLIRQEQK"
     gene            33185..33454
                     /locus_tag="PKK75_01115"
     CDS             33185..33454
                     /locus_tag="PKK75_01115"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNZ82224.1"
                     /translation="MELDKARAEIDKLDKEIVKLLEKRFNLASEIGFLKKEQNLPVYD
                     ESRERQVLEYCAGILENKEYSKYICDIYFQIMNICKDIQRLKDKE"
     gene            33451..33951
                     /locus_tag="PKK75_01120"
     CDS             33451..33951
                     /locus_tag="PKK75_01120"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNZ82225.1"
                     /translation="MKNILIIGMSGSGKTTAGMMLSEKLNMEFVDMDDYIVSVENKSI
                     IEMFEISEEYFRNAESKCSKILSGETSLVIAAGGGIVKRKENMDCFEKNSIIVFLNRP
                     PEKIIEDIDIKTRPLLKEGRDKVFTLYNERIHLYRKYCHIEVLNDTTLDDAVDEIINR
                     VRTCLG"
     gene            complement(33995..35560)
                     /locus_tag="PKK75_01125"
     CDS             complement(33995..35560)
                     /locus_tag="PKK75_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HNZ82226.1"
                     /translation="MSGETLQIFASMIIYMAVVIGIGLYFAKRANESSENYFLGGRTL
                     SPLVTAMSAEASDMSGWLLMGLPGVAYWYGLSDAVWTAIGLAAGTYLNWLFIAKRLHN
                     YSVAAGNSITLPDFLSNRFKEKKKIIMTISAVFILVFFIVYASSCFVTVGKLFNALFG
                     FRYQYMMILGAAFVLFYTFIGGFLAESASDTMQAFVMIIALVTILAVGTYAAGGTAQV
                     IENAKEIPGFFEFFGIASPAVTDGVQQVVNGVPQYNAAGEYGMYTVLSTLAWGLGYFG
                     MPQVLLRFMAIRNPGELKTSRRIATVWVVISLGAAVAIGIIGRAVFPTALLTYSDSES
                     IFIVLSSNLLPAFIAGFVMAGILAATISSSDSYLLIAASAFSKNIYQHLIKKDAADKQ
                     VMIVSRIILVVICIIGIFIALDEDSVIFTIVSFAWAGFGATFGPIIIFSLFWKRTSRE
                     GAVAGMLSGGIMVFVWKLILKPLGGIFGVYELLPAFIISCIFIYGVSKMTHEPSEEIQ
                     NEFETVRKMTLMS"
     gene            complement(36091..37572)
                     /gene="malQ"
                     /locus_tag="PKK75_01130"
     CDS             complement(36091..37572)
                     /gene="malQ"
                     /locus_tag="PKK75_01130"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNZ82227.1"
                     /translation="MERKRSSGILMHISSLPGEYGIGDFGKEAYSFVDFLADAKQKYW
                     QVLPLGITGYGDSPYQSFSSYAGNPYFIELDEFIERGYLTKDEINSIDLGCDVTRVDY
                     GLLYRNKMHLLEKAYEKAKEDLKEDLYRFYWDNNEWLKEFALFMSIKKHHGNVSWLIW
                     QEEYRRKDSKVVEKFEIENIDRIYFWTFTQYFFYKQWYRLKSYANVKGVRIIGDLPIY
                     VAEDSQDVWSNPRLFNLDEDLLPITVSGCPPDAFSRKGQLWGNPIYNWSEMEKDNYRW
                     WKNRIKHSFEIFDVLRIDHFRGFEAYWEIKYGFEDAVIGHWVKGPGTDFFNSIKEELG
                     ELNIIAEDLGFLTEDVKKLLKYSGFPGMKVLQFAFDSREESDYLPHNYDKNCVVYTGT
                     HDNSTVEGWFLNAGIEDVDYAKKYLKLDSKEGLNWGFIRGAWSSAAGLAITTMQDILG
                     LGDEARMNTPATVNGNWTWRMKKTDLTDEMINNLAAITAIYRR"
     gene            complement(37681..38718)
                     /locus_tag="PKK75_01135"
     CDS             complement(37681..38718)
                     /locus_tag="PKK75_01135"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNZ82228.1"
                     /translation="MILKELKIGDLTAKIPIVQGGMGIGVSLANLAAAVANEGGIGTI
                     SGVQIGFREPDFLNNPVEANLRAIGKEIKKAREKSKGIIAMNFMAAMNNYELYVKKAV
                     EEKIDIIVSGAGLPIALPKLTKGTNTKIAPIVSSAKAAKIIIRSYLKSDRLPDAIIVE
                     GPLAGGHLGFKMDELLENKCMPLKDIVKEVKSLLRAFEEEYKVKIPLIAAGGIRTGED
                     ILELEEAGADGFQLASLFVPTIECDAHENFKSTYVNADDENIKIIKSPVGMPGRAIET
                     NFLKGAGKVILTRCYRCMPGCNPREIPYCISEGLINSVEGRDGLVFSGANLNDVNKIT
                     TVKEVINSLIC"
     gene            complement(39088..>39347)
                     /locus_tag="PKK75_01140"
     CDS             complement(39088..>39347)
                     /locus_tag="PKK75_01140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNZ82229.1"
                     /translation="KLAAVEKEVQLLAAEIEKTRRRVNALEYVMIPQLEETIKYIQMK
                     LDENERGNQTRLMKVKDMMVQEAIEEKQQFTKDAFDEYVNK"
BASE COUNT        12485 a         7415 c         7420 g        11927 t
ORIGIN      
        1 gaaacaggag taggagatat taaaatgtct ataaatgatg attcagggta tcatgcaact
       61 attaatgaat ttatgaaaga tgaaagaatt caaacaaaaa gtgaccaagg taccaacatt
      121 aatcttaccg caggagtagg agaaataaat ttaactgata tataacacag atataaatca
      181 tttgtcattc taaaccaggc atggatggtt caggatgaca atttttttgt ctaaacttgt
      241 ccaaaagtag gtataatata gtaataccca gaaataaacc tttatatatt ttcactttta
      301 atgtataatt agaatacaag ataaacaagt atttagaaag atagtattct aagggggaga
      361 ttatgaaaac taatgaatca ttgccggtat acttatttca ccagggtaca aactattatt
      421 catttaattt tttaggctct catattatag aaaatgcagg aactgttttc agagtctggg
      481 ctccccatgc aaaacaggtg catcttgtgg gcaatttttg ctcttggcag aaaaatcccg
      541 gatatgaaat gaaaaaaata cctgaaagcg gcatatggga gttatttgct ccggatataa
      601 gtgaaggaga gctgtataaa tatattatta ctgcagcaga taatgctctt atatataaat
      661 cggacccttt tgcattttct tcacaaacaa agatggaaac agcatcaatt gtgtacaata
      721 tagacaaata caattggaat gatataaatt ggcttgaata taaaaagact gttaatatat
      781 atgagcttcc cgttaatatt tacgaggtta atctgtcctc ctggaaacgc aaagaaaacg
      841 gggactacta cagctataag gagctggctc ccctgttgat agaccatgta aaaaaacact
      901 ttttcactca cattgagctg atgcccgtca tggaatatcc ttttgacggg tcctggggtt
      961 atcaggtttc aggctactat gcacccacca aacggttcgg cgagcctgac gaccttaaat
     1021 atttgattga ccaatgccat caaaatggaa tcggggttgt tctggactgg gtgcctgctc
     1081 attttcccaa agatgagcat ggattgtata aatttgacgg tgaatatctt tatgagtaca
     1141 aggatagcca aaaacgagaa atcggaagct gggggaccca taaatttgac ttcggcaaaa
     1201 ctgaggtaca atcatttttg atatcaaatg ctgtattctg gcttgatgtt taccatgctg
     1261 acggcttgag ggtcgatgcg gtaagctcaa tgctctatct ggattatgat aaaaaggagg
     1321 gtgagtggac tccaaatgta tatggaggca atgaaaacct ggaatccatt gcctttttaa
     1381 aaaaacttaa tgaagcaata tttagaaatt atccaaatac catgatgata gcagaagagt
     1441 ctactgcctg gcctatggtg acaaaaccca catatttggg agggctgggt tttaatttta
     1501 aatggaatat gggctggatg aacgatatga tggagtatat ggaaaccgac ccgtttttta
     1561 gaaagtacaa acataaaaac ataacctttt cacttcatta tgctttcagt gaaaattaca
     1621 tactgccttt gtcccatgat gaggttgtcc atggcaaaaa atccttactc aacaaaatgc
     1681 ccggtcctta cgaagagaaa tttgcaaatg tgagggttct gttaggctat atgattgctc
     1741 atccgggaaa gaagctgaat tttatgggat atgaagtggg acaatttgtc gaatgggatt
     1801 tcaacaagga aattgaatgg tttatgactg attatgaata ccatggaaag ttgaatacat
     1861 atattaaaga attgaatgaa ttttatctga aacagcctga actttgggaa gtggattatt
     1921 cctggaacgg gtttaaatgg atttctaacg atgattttga tcagaatatt atagcattta
     1981 aacgaataga caagaataac aatgaattaa ttgcggtggt taatttttct cccgcagaaa
     2041 gacaaaacta tttgattggt gcagacagag gaatttacac cgaaatattc aattcaaatt
     2101 gcgaagaatt tggagggacc gggtcaggca acaaggataa aatcgtatca tcccaaggga
     2161 gcttacacgg acatgaatct tatttatctt taactttgcc acccctttca ataatatttt
     2221 taaaaaagga aaataaatat aatataaaca acaaaggagg taaaatttat gaaattagct
     2281 aagaaagaat gtgttgcaat gctccttgca ggagggcagg gcagcaggct gggcgccctt
     2341 actactaaaa ttgcaaaacc ttctgtaccc tttggaggga aatacagaat aattgatttt
     2401 gccttatcaa attgtgtcaa ttcaggtatt gataccattg gagttttgac acaataccaa
     2461 ccattaacgt tgaattcata tatagctgac ggaaaatcat gggatttgga cagaatgtac
     2521 ggcggagtcc aaatactgcc cccatatcaa agggtggctg actcagagtg gtatctgggc
     2581 acagcaaatt ccatctgcca gaacatgaat ttcatagatc agtatgctcc tgaatatgtg
     2641 cttattttat ccggcgacca tatttataaa atggactatt ccttaatgat agattaccat
     2701 aagaaaatgg acgccgactg taccatatcg gtaatagatg tttccttaaa ggaagcttcc
     2761 cgttttggaa ttttaagctc ggacgagact ggaagaataa ctgattttga agaaaaacca
     2821 aaaaaaccaa agagcactca ggcatccatg ggtatatatg tatttaagtg gaacaagctt
     2881 aaaaaatatc ttgaaataga tgataaaaat cccgattcat ccaatgattt cggcaaggat
     2941 gttattcctt taatgcttga aaaaaacgaa aaaatgtatg cctacaaatt taggggctat
     3001 tggaaggatg ttggaaccat tgattcgctg tggcaggcaa atatggacct tttaggggaa
     3061 aacggctttg aattatctga agaaaattgg ataatttatt caaggcatgc ggaaaaaccc
     3121 cctcattacg ccggcaggga tgcagtgatt aaaaattcga ttgtttcaga aggtgccgta
     3181 attttaggaa ctgtagaaaa ttctataata ttttccggag taagggttgg agaaaatgcg
     3241 gttgtaaaag attccgtaat aatggctgat gtagaaataa atgataactg cgtagtaaat
     3301 tattccatca ttgatgaaat ggtaaaaatc ggaccaggcc ttaaaatagg caaaccaatg
     3361 gaggaaaact ctgaaattac ggtaataccg agggaaagta ttataaataa tatgggaggg
     3421 ataatatgaa tgctttagga attatttttt ctgatattca agattggagc atgaccgaac
     3481 tggcgtctca caggtctgtt gccgcaatac cttttggagg caggtacagg cttatagatt
     3541 tccccctttc aaacatggtt aattcaagca tcaacaaggt ttacctaata acaagcagca
     3601 actaccgttc attaatggag catattggta caggaaagga ttgggacttg tcaagaaaaa
     3661 agggaggcat acttactatt cctccttatg ctgcaggcga aaaccacggt ctctatcagg
     3721 gtagactgga tgccttgaaa agagttttgg attatataac atcctcaaaa gaaaattatg
     3781 ttgttttatc agattctgat atgatttatg acataaatta ccaaaaggtt ataaaatacc
     3841 atgaagaaaa aaattcggat attactgcag tctacacaaa gataaacata aataaatctg
     3901 aatcaattca caaaatatta tatgaatttg accaggatgg aagagccgat gacatattgg
     3961 tttatcccat aacaaacgga attcacaatg tgggcttgaa tacatgggtt atgaacaagg
     4021 aatttttgga atcccttctt tatgacgcta ttgcaagaaa ttacaaggat ttcagcctta
     4081 atgtactgtc agccaaggtt cattccttaa gaatttacgg ttatgaatat accggcttct
     4141 ttgcacatat tgattctctg caaagctatt ttgaaaacag catgaaatta ttgaacattg
     4201 ataatctgca aaatattttc tattcaaaaa caggtcctat ttatacaaaa acaaaggatt
     4261 cagcccccac aaaatatagt aaggatgcag ttattaaaaa ttcactgatt gccaacggct
     4321 gtgtcattga gggaacagta gaaaactcaa tcctattcag gaatgtgaga ataggcaaaa
     4381 atgcagttgt taaaaattca ataatcatgc agcacggaat aatcagtgat aattcaaagc
     4441 tggactccat aataaccgac acctacacat ttgtaagcaa ggacagggtt ttgctgggat
     4501 attcaaactg ccccttcttt ataaagaaaa actcagtggt ataggagtga tttatatgag
     4561 taaattaaaa gtattatatg cagcctcaga ggctgctccc tttattgcaa ccggaggtct
     4621 cggagaagtt tcggggtcac ttcccaaggc attggcagaa aaatataaag ataaaataga
     4681 tataagaata attcttcccc tgtatcaggg aattgaagac agaagcgaat ttgagttttt
     4741 gggaaaaacc tctgttcctc ttgcctggag gcagcaatac tgcggagtct ttaaaagggt
     4801 tgtggaaaat gtcacctatt atttcataga caatgaatat tattttaaac gcagtgaatg
     4861 ctacggtcat tttgatgatg cggaaagatt tgcatttttc tcaaaagctg tactttacat
     4921 ccttcctatg atagaattca tgcctgacat tattcatgca aatgactggc agacatcact
     4981 ggttcccatt tatctgaata caaagtattc cggctatgaa ggatacagag atataaaatc
     5041 aattataacc attcacaaca ttgaatacca gggagtgttt gatttaagca tttcagagga
     5101 tgtattcgac ttgcacggaa aagaaaagag tataattgaa tatagaggag caataaacct
     5161 cttgaaggga gccttggaaa cagctcatat tattagcaca gtcagcgaaa gctattcaaa
     5221 agaaatttat gatgattatt atgctcacgg tctggctgaa attattcaaa aaaattcatc
     5281 caaggtgcgg ggaatactaa acggtataga tgctgaaaag tacaatcccg aaaacgatcc
     5341 tgaaattttt gaaaattaca ctgcaaaaac aataaaaaag aaatatttaa acaaaaaaaa
     5401 cctccagtca ttatcaggtc ttccgcagga taaagatgtg cctgttatag gaataatatc
     5461 aagattggtt aaacataagg gaattgatct gataattaat gccatagaag gaatactaaa
     5521 agaaaaagtc cagtttatta ttcttggcaa gggagacaga ggatatgaac tttactttaa
     5581 atatttgcag gaaagttatc atgataaaat ttacgtaaaa atcggctttg attcaagttt
     5641 tgcaaaaaag atttacgcag gctctgattt atttcttatg ccttccataa gcgaaccctg
     5701 cggcatagca caaatgattt cctcaagata cggggcagtt cctattgtaa gggaaacagg
     5761 aggtcttagg gattcaatca aggacgcaag ccttggtgaa ggaaacgggt ttaccttcag
     5821 cgaccagact cctgaagcat tgtgtgatgc cgttaaaagg gctttgaaag tttatgaaaa
     5881 taaagaagac tggaaaaggc tggttgaaac agtaatgaat actgatttca gctggaaaaa
     5941 gtctgcagaa aagtattttt atatgtatag cagcttagca gatataggag aatgataatg
     6001 aggtcaaaaa tcgacgcaag acaaattaac cgcctaatcg atgaggaaat cataagatat
     6061 tggaatacag aacctgaagc agccagtata aatcaaatct acagggcatt atcctttgta
     6121 ataagagatt tgcttttgag aaaaagaaat aaattcaaaa gaatgcaaaa tgaacacaaa
     6181 caggtttact acatttgtat ggaatttcta ataggtaaat cacttaagaa taatttattt
     6241 aatttaggta ttgaagaaga atcaagagaa gctctcaaga aatataatat aactcctgaa
     6301 gaattatatg ctatagaaaa ggacccgggc ctgggcaacg gaggtcttgg aaggcttgcc
     6361 gcctgtttta tggattccct tgcaacattg gactatcctg cagttgggtt ttctataaaa
     6421 tatgaattcg gactttttaa acaaaaaata atcgatgggt ggcagacaga actgcctgat
     6481 gactggctta ccacttccga tgtctggctt atgcccagga cagatgaaag agttgttgta
     6541 aaatttgatg gtgcaataag agaagaatgg acccgggaag gaatgaaagt gcaccatgaa
     6601 ttcgctacgg aagtagaagc aataccttat gacgttatga tttccggata tgacagcaag
     6661 ggagtaagcc tgttgaggct ttgggagtca agaagtttaa acagcattga tatgaacctg
     6721 ttttcacagg gtgactatat aagagccttg tctgaagact caatggcaga agcaatatca
     6781 aaggttctgt atcctgctga tgaccataat gagggtaagg tgctaaggct aaagcagcaa
     6841 tattttttgg tttctgcctc tgcacaaaat attgtcaaat accatttaaa taaatacaat
     6901 agcttggata atttcagcga aaaaaatgca atccatataa atgacaccca ccctgctttg
     6961 ataattcctg aattaatgag gatttttatg gatgaatacg gattatcatg ggagaaggcc
     7021 tggaatcagg tagttaacac agtatcctac acaaatcaca cagtattgaa agaagcactg
     7081 gaaacatttg acgaaagcat gatacaaaca aagcttccaa gaatatatat gataataaga
     7141 gaaataaaca acaggtttct tgacgaagca gcaatgaaat atgaaaatta cagcaagccc
     7201 ttgaatatgt ccatatttgg ctatggaagg gtaaatatgg ctaacttgtg cataatcggc
     7261 agccacaatg taaacggggt tgcaaggctc cattcagaaa tattgaaaag aacagtcttc
     7321 aaacactttt atgattacac tccggataag tttatgaatg tcaccaacgg tataacccac
     7381 cggcgatggc ttaatcaggc taacccttca cttgaaaaac ttcttgatga acttattgga
     7441 cccggataca gaaagcaacc ggaaagactt ggtatgttta agcatttcaa ggatgacgac
     7501 ggtgttttga acagacttga tgaaatcaag tatataaaca agaggaatct tgttgagtat
     7561 gtgagagcta acaatcatat tgagcttagt atcgactcaa tttttgacat acaggcaaaa
     7621 aggcttcatg agtacaaaag acagcttctt aatgcactta ggattataag cattataatt
     7681 gatataaatg aaggcaatac cagggatata gttcccgaaa catttatttt tgctgcaaag
     7741 gctgctcccg gctaccatag ggcaaaggaa ataattcagc ttatttatgc attgagcgaa
     7801 tatataaaca aaaacacctt tatcaaaaaa tttatcaatg tagttttcct ggaaaactac
     7861 tctgtaagtt tagctgaaat aataatacct gccggcgaaa taagcgaaca gatatctttg
     7921 gcgggaaagg aagcaagcgg aacaggaaac atgaaattca tgcttaatgg tgcattaacc
     7981 ataggaacat acgacggtgc caatgtggaa ataataaatg aagtgggaga agaaaatttc
     8041 ttcttgttcg gcttaaggga ctatgaagtt gaagaattgt ggaaaagcgg atattcatcg
     8101 gtagcctatt atcaaaaaaa tgagaagctc aggaaaataa ttgatttttt aaacagagga
     8161 attgcaggaa aaccctttga atccatatcc agatacctta taggcggacc cgtggtaggt
     8221 gacccctaca tgtgttttgc agactttgac agctatatga aggtgcatga aagggcatca
     8281 ttcctttatt ataacgataa aaggaagtgg aaccaaatgt cattgataaa tattgcatct
     8341 gccggagtgt tttcatcaga cagaacaata aacgaatatg caggcaaaat ctggggtatt
     8401 aagccgatat agcagaagca tggggatata cctctttaga ggcgggtaca agccatcacc
     8461 cttaaggtta aaagttgaag gaatgtcccc ctgcctggtc tttgcggtta agaggtgcgc
     8521 ccccggattt aggaggtatt atgtataaat ttaattctca gtctgaattt tacagacatc
     8581 ctcagggcgg aattaaatca aacgaaataa taacatttaa aatatatgta aagagaaact
     8641 cgtccaggcc tgaactgctg attgaaaaaa ggcataacca tgaaaagtct ttttacggca
     8701 aaataaaaat gcagtggata tcacaagaaa aaaattatga tctctaccaa gctgaggttt
     8761 ctataaagga atacgggaat tactattatt cctttatttt taatgaagag accaccagag
     8821 actatgaact cctcatatat gatgaaggct atttaactcc ggactggacg aaaggtggaa
     8881 tcatatatca tatttttgtg gacaggtttt tcagaagaaa tataatcaaa aaaaatggag
     8941 caataataag aaagtggggg gaaattcctt actatctgcc taacacggaa ggtgaaatct
     9001 taaataatga ctttttcgga ggaaacctgg aaggcataag ggaaaaactt ccctacattg
     9061 caaaattagg tgtgagcatt atctatttat ctccaatttt cgaagcatac tcaaatcata
     9121 aatatgatac aggaaattat cataagatag accctatgtt cggcactgag cactccttaa
     9181 aaaaactcat acgagaagca aaaaaatacg gtataaatat tatccttgac ggtgttttca
     9241 gccataccgg ggatgacagc atttatttca acaaatacgg caggtataat tccatagggg
     9301 catatcagag caaagattcc ccttattaca attggtatac cttcaataat tggaatcatg
     9361 actatataag ctggtgggga ataaaaaccc tgcctgaaat aaacgaaaac aatgactcct
     9421 atgtagactt cataaccgga aaaaaaggtg tcatccacca ttggcaaaaa gcaggagtta
     9481 tgggatggcg tttggacgtt gctgatgaac ttccggatga tttcatatct gcccttagaa
     9541 gttcaataaa agaatataac aatgatgcgc tgataatcgg tgaagtgtgg gaagatgctt
     9601 cgaataaata ttcctatgat aagctgaagg aatatttttg cggcagacag ttggattccg
     9661 taacaaatta ccctcttcgt gacggcataa tccaatttat aaagagcagg gactgcactt
     9721 atcttcataa tataatgaat aaaattatag aaacctaccc tccgcaaact gtaaactgcc
     9781 tcatgaatgt tctgggaacc catgacacgg aaagaatact cacagtactt ggcagcgaaa
     9841 acctgccttc taataaaatt gaaatggcag aaagcaagct ctcaggggag gaattaaaac
     9901 aaggaaccaa tctcttgaaa attgcagtgc tgctgcagat gactcttccc ggtgttccct
     9961 gcatttacta cggtgatgaa gcaggcatgg aggggtggga agatcctttg aacaggagat
    10021 gctacccctg gggaaatgaa aacgaagaaa tactttccta ttatatgtta gtgacaagct
    10081 ttcgcagaaa aaacaaagtt tttgctgaag gtaagtacag atgcttaatt catgataaag
    10141 gcgtttttgt gtatgaaaga tatgatggaa atgaaaaaac aattatagga gtaaatttat
    10201 caaaaaagga aataacttta aaatttaaag aaaatatgac tgaatacaca aataatataa
    10261 aaaataatgt tttcaatata gaaaaggaag ggtttctaat tttaatttag tctgccctgc
    10321 cttttcaaaa aaccttatgg ggacacacat ttaaattgaa ggtgtgtccc cataagttta
    10381 aagatatttt ttcagcacgt cctttttgtc tgtcttttcc cacggaaggt caaggtcttc
    10441 ccttccaaag tggccatatg ctgccagctg ctggtatata ggtcttctta aattgaattt
    10501 ttctataata actgcaggtc ttaaatcaaa attctcggct attgcttttt ctattatttc
    10561 atcggcaacc ttgtttgtgc cgaaggtttc aaccattatt gaaaccggtt ttgcaactcc
    10621 gattgcatat gcaacctgca cctcgcattt gtctgccaat cctgctgcaa ctacattttt
    10681 ggcaatatat cttgctgcat atgctgccga acggtcaacc tttgtagggt cctttcctga
    10741 aaatgctccg cctccgtgtc ttgaatatcc gccgtaagtg tctacgatta tctttcttcc
    10801 tgtcagtccc gaatcaccct ggggtcctcc tattacaaat cttccggtag ggttaacgta
    10861 atacttggtg ttttcatcta ataattcggc aggaacaatt ggtttaacaa caatttcaat
    10921 taaatcttcc gcaattgttt ttatgtcaac ttcaggatta tgctgagtgg aaataacaat
    10981 tgtatcaact cttacaggct tgttatcatg atattcaaca gtaacctgag ttttcccgtc
    11041 aggtcttaag tagtcaacta ttttgttttt tctgacctca gcaagtttct ttgaaagctt
    11101 gtgtgccagg gatatgggca tgggcatcag ctcaggtgtt tcattgcagg caaaaccaaa
    11161 catcataccc tggtctcctg caccaatcct gtcatttgca tcattatgat cctttttgta
    11221 ttcatctgcc tcattgacac ccaatgctat gtcctgggat tgctcttcaa ttgatgtgaa
    11281 tacagcacat gttttgtaat caaagccgta ttcggcattg tcatagccga tattttttat
    11341 tgtgtttctt actatctgcg gaatatcaat ataacaggat gtgctgattt ctcctgccaa
    11401 aagaacgaat cctgtcttgg tgattgcttc gcaggcaacc cttccattgg gatctaattc
    11461 cataattgca tccaagatgc catctgatat ctgatcacac attttatccg gatgaccctc
    11521 tgtaaccgat tcagatgtga ataaccattt tttcattatt ttatcctttc tttgtataaa
    11581 tatattcttg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagaagag
    11701 gtcaattttt cctattgcct catctttcgg aaatccgcgg gaattggcac cttgaataaa
    11761 tcaggctgcc gggtttcatc gggcctcgtc cctccaccac tcttgataag gagttttttt
    11821 tatgttaaca catacatttt ttattgtcaa tcattctttt attttcatgg aacctatatt
    11881 attataattt cgtctatata atagtatggt cataacctga agcattctaa cataaaataa
    11941 taaaaccctg tttttctata atttaccgtt tcaatatttc aaaacctatg gaaagcaagg
    12001 cagaattttg atataatttt aagaagagaa agggtggaca agatgatact aaaaaagata
    12061 gcggctattt ctgccgcagc aatactgctg ttatcctctt cagcatatgg cagcagtcta
    12121 tacactgtat atgatttatc tgaagaaaca aggctctcaa agagtattac atatgaaaga
    12181 acagaaaaat acacatcgtc agggtggatg aacataaatg tcatccgggc agacctgact
    12241 gatgaccata cagaaataaa acctgtaacc aatgaagacg gcatttccaa cagagcggtt
    12301 ttaagctcaa tgataaagtc atcgggagca gttgccgcag taaatggaga ctttttttac
    12361 atgggtgacc ctacatatgc atacggtcct ataatcagag acaataaatt aataacctct
    12421 cctcttcctt ttagctacgg gtatcccacg gtttcaaagc ttgaagataa gattgatatt
    12481 tcagtatggg atcccaaaat tactctttac ggatcagatt ccactgaatt caatgttact
    12541 gtggtcaaca agacttcctc ccttgaatgg ggtcccacca ttcttacatc agactggagc
    12601 aatacatcac ccggatacac aacaaaagat attgtggagg ttgttatagt tgataacact
    12661 gtatcccagg ttagaaagaa tcagccttcc acggccattc ctgaaaatgg ttatataatt
    12721 gcaagctcaa atgctgcaac aatggacatg atggaaaatt cattcaggga aggacgccct
    12781 gtatccttga atattgaact tgactttgac ccggaggatg ttgactgggc ttttggcgga
    12841 ctgaattacc tggttaagga cggcagggaa aatgagataa gctcccaggt ttcaggagca
    12901 aatccaagga ctgcaatcgg attcagcatg gataatactg aaataattat ggttactatt
    12961 gacggcaggc acaaggatta tgttggagcc ggtcagaagg agcttgctaa aataatgatt
    13021 gaattaggag cctacaatgc agttaacatg gatggcggag gctccacaac catgggcatt
    13081 gattttttaa gaaatgcaga tgttactgtg gttaacattc cttcagacgg cagagaaaga
    13141 agaattgcat caggagttgg tgtttttaac aactaccctg acagccgcag agtggaaata
    13201 atagaaataa aagctgagca aaataaagtt tttaataaaa cagaaacccc tttggagctc
    13261 aaatttttca atgagtacta ctcccctgtt gactatgatc tgaaaaatgt agattttact
    13321 gtatatcctg caggagccgg aagggttgca aacaatgtat tcatgccgga aaggccggga
    13381 aaggcaacaa taaccgcccg cgctggaaat gcagaaggac atattgaaat agatgtttta
    13441 gacaaggccc aggcactgga agtggaaaca gattccttaa ccctgggatt caatgacaaa
    13501 tatgaactgg gccaagtcat gggaattgac aaatacggca attctgcata cattccatgg
    13561 aagtacattg ctattacata cagaaacaaa gtgggaaaag ttgaaaacgg tttattcaca
    13621 tcaagtgata tttcaaatac cggggcagta acactaacct tcggagatgc tgtaaagcat
    13681 atacaggtta agacaggcta cagatataaa accttgaatc gttttgaaaa ccttgataat
    13741 ttgaaattga ccttatatcc tgaggattca tacggctata tggccattag caaggacttt
    13801 gtcaaagaag gggaaaatgc acttaagtta gattacgact ttacaaaaat gacagatcaa
    13861 agtattgctt ttgtggaatt tggcagtgac gggcaaggaa taaagcttga agacaagcct
    13921 ttggcaattg gtatgtgggt ttatggcgac aataaaaacc actggctcag aacaagaata
    13981 accgatgcct acggcaccca ggtgaaaatt gattttgaag aagaagtaaa ctggactgga
    14041 tggaaatggg ttgaagccca tatacctgaa ggcattgctt accctattac actaaaaaat
    14101 atatatcttg ctgaaattaa cgaaacaaga aaagattcgg gaacaatata cattgacaat
    14161 ttaagaatac tgtatgagcc aaaggacaag gaactgggtc tcagggctga aaccctgttt
    14221 gaagatacta tgaaaagctc tgcaattaca aattattccg agaaattaac catatctcag
    14281 ggtaaatata agtatgaagg cactgatagt atcatacatt gcgaaggtat aatttcaaac
    14341 ggcacaatga gtgcggctga tattacaatg tggagcaaca tcaagtcatt tatggaattt
    14401 gaaaacaagg tattggtttt atccctgaac agccatattc ataatattta tgacaaaaga
    14461 gaagtgagaa ttttgaagga aattctcgaa aaggcatcac aaaaaaatga tgtgtttgta
    14521 gtatttaagg gagaagacga aaatacagtg attgaaaaca gtgtgaggta tatcacctat
    14581 gatgacagct ttgaaatagg agtatcccaa gacagagttt catataaaaa ttaactttat
    14641 acccaatttt atgttgatat ttcataaaat tgggtataat atctaagaac aggggtatag
    14701 gtatgtacct gccgctggtt aatattatat actcggagga aattatgtgg aaagaattta
    14761 aagagtttgc attcaaaggc aatgttttag atttagcaat tggtgttata atggggggtg
    14821 ctttcggaaa aattgtaaca tccgttgtaa atgatttaat aatgcctata ctgggatact
    14881 taatggcagg aatggatttc aaatcactga agtttgtaat gagtgaggca ataatgcaag
    14941 gcgatactgt tattaagcct gaagcagcaa ttttatacgg taattttatt caaaacattg
    15001 tagacttcct aattgtagca ttatcaatct ttttgtttat cagattaata aacaaatcaa
    15061 aagaaaaaca aaagaaacaa gaggaagccg ttcctgagcc ggaagttcct gcaggacctt
    15121 cacaggaaga actgcttact gaaataaggg atttgttgaa agaaaaataa accggttagc
    15181 ttgtttcaaa aaagcgctct ttatgagcgc ttttttaatg gatttaatgt tgaaatctat
    15241 atttttcatg atagaataaa tatagaatat ttttggagga agctatgagt ttatataaac
    15301 agtggacgga aaatttggaa gcaattaaaa gccagggaga atacaacgat ttttttaatg
    15361 cttatatgaa aaaagaaaca gaatgctaca gaaatcttct tgaaaacaaa aatcctgtaa
    15421 ttgagggtaa aatacaggaa ctggcagaaa aaaacaatat gactcctttg gaattcacag
    15481 ggtttttgga cggtgcaaac acaagtttta aagaagccct tgtcttggat gatttaaccg
    15541 gagattctga aataaaagct gaatttgact ttaaaaagct cttatggaat atgtatgatg
    15601 taaaagccca atggctttac ggtatgaagg aatgggaaaa catctttgat gaagaaaaaa
    15661 gaaggcagat taaaaaagaa tacaatcgtt ccaaaatagt agtaaaaggt gaaaagacag
    15721 gaagaaacga cccttgcccc tgtggctcag gtttaaaata caaaaagtgc tgcggtaaat
    15781 aatgtaggtg ttgtatggat aagcaaaaag gtatagtatt tggcattttg gccgccttta
    15841 tatacggctt cacaccaata ctggggaaac tgacctacct ggaggggagc aacacaatgt
    15901 cccttgcatt ttacagaaac ctgttttccc taccttttct ttttttgatg ttaaaataca
    15961 acaaagttga attaaaaaca gataaagtcc aattaaaaaa attagccctg ctggcaacaa
    16021 tggggccaac tctcaccagc ctctttttgt acggatccta catttatatt acagttggta
    16081 tgtcaacaac catacattat atctatcctg tattggtggc agcagccagc atgcttatat
    16141 tcaaggaaaa gataagcaag gaaaagctta ttgccctttt gttgtcaaca gcaggggcag
    16201 caatgttttt tgaaggagat ttcaatatta caggtgtttt attggcattc tcatccggtg
    16261 ttatttatgc agcccatgta ttattcatag acaaaagcgg cttaaatgaa atttatcctt
    16321 tcaaaataac attttatttg tcaatatttt cctcaattta tttatttatt gcaggtttaa
    16381 ttagcggcag cctggtattc acaataacgc caaagggatg gttattcaca atactggtgg
    16441 cattcttcgt agcattttta gctaatacat ttatacccat aagcataaaa tatgccggac
    16501 ctactgttac aagcattgcc ggattgtttg aacccattac cagcatattt acgggagtat
    16561 tgttcctaag cgagcccctg accttcaaaa gcgtaacagc atgcatactt attttgctgg
    16621 gtgtactgat agtaaccctg acaaaagaga tgccctaacc caatttcttc gaccaaagtg
    16681 aaagggacct tatcagtccc tttttagatg cttatttgtc tgtcatcatt tcacatgccc
    16741 ttacagcctg ttccagcagt attgttgttg ttatggttcc aactccgccg gacgcaggtg
    16801 ttaacatttt tactttgccg aataccttat cgtaatccac atctccgcaa attttaccgt
    16861 ttccatcgtc attcactcca acatcaatta caattgatga ttctgtgaaa tattcttcgt
    16921 tcataaattt tgccttgccg attgctgcaa cgactatatc cgcctgagaa gttatagacc
    16981 tcatatcttt tgtttttgaa tggcatattg tcactgttgc gttttctttt aaaagaagca
    17041 tagccagagg cttgcccaca accaggcttc tccctgccac agccacattt gcacctttca
    17101 aaggtatttg ataatacttc agcatttcaa taactgcctt gggtgtacaa ggtgaaaaac
    17161 cgctgtcatc gccctcaaat atcttttcta agtttacagg tgtcataccg tcaatatctt
    17221 ttacggggtt tattttctcc aagatttcct tttcgttcaa atgcccgggc aagggcctga
    17281 acatcattat tccgtgagta ttatcatcct cattggcatc ttcaatagtt ttacctaatt
    17341 tttccattga aacattttcc ggaagctctt taacccttcc ttcaatgcct aaaagagagc
    17401 agtttttcaa tatgcttctc tcataaaaaa catcatcttc cttgtttcct acccttatga
    17461 taattattgc aggaacaata tgtctttcat taagtcctgc tactcttgtc ttaatatttg
    17521 ccctgagatg ttcagccaca ggttttcccg tcagttttac agtttctaca gacatttttc
    17581 cctcctttgc ctaatgcgca attattggtt taatactgtc agcacctttt gatatacttc
    17641 atctgctact tttgttcccc tatcaacaag tttttctgct ttatcccttt gccctctcac
    17701 ataatcttca tcatccattg aatttaaatt aattaaaaca ttgagatacg cactgtttaa
    17761 tgcagccttc aaaaactgaa cccccacgcc cacatcgctt attatatttt ttgatgagat
    17821 atctaagatt tcctcgtgga gaagtattgc atcatatgaa aattccaatg tttccatagg
    17881 tgctgaacat gcttctatca aatatctctg aagcgtttta tttttcaagg ccttttcttc
    17941 atcggtattt gaaggcatta catatgctct cgacaaaggc tcgaaaactt ctgcatcctt
    18001 atttaccagg tctatgagct taacccttaa tatttctccc ctttttatca gctcttcatg
    18061 tttgtctttt atatcaatga attttttctt accataggag aaatttgcaa ccattgtatt
    18121 caaagctgca cctaacgctg caaccatagc cgcggcacct ccgccgccgg gaacaggttt
    18181 tttggatgcc agttcctctg caaaatcttt gcatgataac tctgttaaca ttattatttc
    18241 accgccttac aggaaatatc tcagtctagt ctttaaaata gaccttattg tatatgaatg
    18301 gtatcaataa ccctccccct atataatttc ccaaagctga aggtattaaa ctgtaaattg
    18361 tcatattcac aatatttatt gacgtatcaa gaaatgatgc catcggaaga taaaacatat
    18421 ttgcaaccac atgttcatat ccgcacagca caaaaagcat aacaggaaac cagcatccca
    18481 gaatttttcc tgtggtatcc tttgaggtaa ttgaaaacca taccgctaaa gataccagta
    18541 tattgcataa tattcccttg ctgacaacct gcataaatgg caaggatagt ttagcctcag
    18601 caatttgaat tatcctttcc gccaaaacag gattattata tacccctcct gcatacaata
    18661 accctgctgt taatgctcct ccgataaaat taccggctaa aacctgcaat agggtgcttg
    18721 tatagtttga ggcttttatt tcacctttca aaagtgcaaa agtcatcagg caatcacctg
    18781 tgaataattc tccgccaact aaaactatga gcatcaaacc taccgggaat actgcagctc
    18841 ttataaacat attgtcaaat gctatgataa agccttgtgc tcctaatgct atgtacatac
    18901 ctcccataat actcatcaat aaacgtttct gaacagtcaa tgaaacttta ttctgtgctg
    18961 cttttattaa ggcttcggca gtttctttag gtgttaaaga tattgaactc aacttgactt
    19021 cccttcttta atattcagag tgtatgctcc tgccgcttat tgaggcagga gcttctcgcc
    19081 ggagtaatct gattattaca atataagccg gctatccctg tgtgtcctgt ggtatacctt
    19141 agaataggcc ttttatttta ccgtcaatgt cgacgtctat attttctgct gaaggtactc
    19201 taggcagtcc cggcattttc attatttctc ccgtcagtgc aacaataaac cctgctccgg
    19261 ctgatattgt tatatctctt attattattt taaatcctgc aggtcttcct ataactttag
    19321 gatcgtctgt aagtgaatat tgagttttgg ccatgcatat cggaagattt ccaaatccaa
    19381 gcttctccag attttctatt tccttgtctg ccttcttcga atattcaacg gattcagccc
    19441 cgtaaatctt tgtagttata gcttctattt tttcttttat tgacatttca tcggtatatg
    19501 gaaattccat gctgctttct ttttctgcca gccttataac ttcttcagct agttctttgc
    19561 ctcctgatcc gccttttccc caaacctccg acaaggcaac attaactcca agctcgctgc
    19621 atctttcctt tataaggttt aattctgctt ccgtatctgt ggggaatttg tttattgcaa
    19681 ctacagcagg aattttaaac ttctgtgtaa cattctccac atgctttaac agattaggaa
    19741 gtcccttatc caatgcttcc aggttctctt ttcctaaatc tgcctttttt acttctccat
    19801 ggtgtttcaa tgctctaacc gatgccacaa taacaacagc gttaggtctc agaccggtct
    19861 ttctgcattt tatacctatg aatttttcag cacccaggtc tgctccgaaa cctgcttcag
    19921 ttacagtata atctgataat ttacttgcca tttttgtagc tatgacacta ttgcagccat
    19981 gtgctatgtt tgcaaaaggt cccccgtgga taaaagcagg cgttccttcc agcgtttgta
    20041 ctaaattcgg ctttaatgcc tccttcaaaa gtgcagccat tgcgccttct gcttttaatt
    20101 gtcccgctgt tacaggattc ccttctcttg tgtatgcaac tattattttt gaaagatttt
    20161 cctttaattc catgatgtca ttggacaagc aaaatattgc cattatttcc gatgcaactg
    20221 taatgtcgaa cccgtcttct cttactactc cttcagttct tcctccaagg ccgtctacta
    20281 tgtgtcttaa ttgtctgtca ttcatatcaa cacatcttct ccatactatc cgccttgtat
    20341 ctatgttcag ttcattgcct tgatgaatgt gattatccat cattgcagcc aaaagattgt
    20401 ttgctgcacc tactgcatga aaatctcctg taaagtgcag atttatatcc tccatgggta
    20461 cgacctgagc gtatcccccg cctgccgctc cgccctttac gccaaaaaca gggcctagag
    20521 aaggttcacg aagtgtgata attgtattct tgccaagcct tgctaatgca tcccccaggc
    20581 ctattgttgt tgttgttttg ccttcacctg ccggtgtggg tgttattgct gtaaccagta
    20641 ttaacttggc atccttattg tcctttaatt cattctgcaa atatctgtaa tcaattttag
    20701 ccttgtattt accgtaattt tctacatact tatcgggtat tcccaacttt tctgcaatag
    20761 tgtttattgg tgcaattttt gtctgctgag cgatttctat atcgcttaaa aacgtatctg
    20821 gcatataatt ctctcctttt attctattgt ttgcttaaat taatataagt ttaatataac
    20881 ttagattatt aaaatgaatt atatattaat tatatttcta agtcaatcaa attcgaatta
    20941 ttatcctgga caatgaataa acctgttaaa atataaacaa aactatttgt accgttttta
    21001 acatttcctg acacctccgc catgccggta attttattta atataacaaa agatttgatt
    21061 attggaacat tatttgggta taatgaaata atgaaaacta agaatagtat tattagtttt
    21121 tcctatatta aacatgttga cttttaagta ccgttttaat atagtataca taaaaataaa
    21181 aaggagtgat aaagtgacgg caaaaaagac accgctttat ggcgaacatg ttaagttagg
    21241 cggtaatgta gttgactatg ccggctggtt tcttccggtt caatataccg gtttgatacc
    21301 agagcatgag gctgttagga atgcagcagg aatttttgat gtatcccaca tgggggaaat
    21361 caccgtaaaa ggcaaggatg cagtagcttt tgttgattat cttatgacta atgacattac
    21421 aaaaattgtt gataatcaaa taatctacac gttaatgtgc taccctgacg gaggagttgt
    21481 tgatgatttg ctggtatacc ggtatgaaaa caactacctt tacctggtag ttaatgcttc
    21541 aaacactggt aaggattatg cttggatact tgagcataag ggtgattttg acgtagaaat
    21601 cgaaaatatt tcagattcag taggcgaagt agccctccag ggaccatttg cacaaaaaat
    21661 tcttcaaaaa ttaactgatt ttgatcttga caagatagag ttcttccatc ttcaaagaga
    21721 tatttcagtt gatggagtaa actgcatgat atcaagaacc ggatatacag gagaagatgg
    21781 ttttgagatt tatgctgata atgagggtat agtaaaggtt tggaactcag ttctagaagc
    21841 aggtaaggaa ttcggcatac ttcctgcagg cttgggatgc agggatactc tgagattcga
    21901 agcttctctt cctctttacg gacacgaaat cagccaggaa atcactcctc ttgaaggcgg
    21961 gttcaagtac tttgtgaagc ttgataaagc agctgatttt attggcaagg aagcccttaa
    22021 caaacaatgg tctgaaggct tgaagaggaa attagcagga tttgagctta tcggaagagg
    22081 aattgcaagg gaaggaatgg aagtacaaaa agacggagtc attataggac atgttacaac
    22141 aggatacatg agtcccacac tgaaaaagac aataggcaac gctttactgg cttctgaatt
    22201 tacggagctt ggtacagaaa ttgaagtaat ggttagaaac aaacctgtaa aagccaaggt
    22261 aataagcaag aagtttctta agaaaaaata aactgtaaaa gctaaatcaa ggaggattta
    22321 aaatgaaagt tgtaaaagga ttatattatt caaatgacca tgaatgggtt aaggttgatg
    22381 gagacgaagc atatgtaggt attgcagact tcgcacaaga gcatctggga gatatagttt
    22441 atgtggagct tcctgaagtt gatgatgaat tcgcaaagga ggaagccttc tcagttgttg
    22501 aatccgtaaa agctgcatcg gatatttatc ttccggtcag cggtaaggta atagccgtta
    22561 acgaagcttt agaggatgaa ccggctctgt taaacgctga tccatatgaa aactggatta
    22621 ttaaaatagt cttgaatgat aaggcagagt tggatgaatt gatgacaagt gaggattatg
    22681 aaaaattctt ggctgaggag gagtaatatg tatccataca taccaaatac tcctcaggac
    22741 gaacaggaaa tgttaaaaac aatcggtttg gattcaataa accaattgtt tggagacata
    22801 ccggagcagg ttcatttgac aaaaccgtta aacattcctg attcaaagag cgaattggaa
    22861 gtaagcggtt atcttaagaa cctggctgat aaaaactgcc cggtcagcca attggcatgc
    22921 tttttaggtg caggtgcata tgatcactac attccatcag tgataaatgc aattacttca
    22981 agaagcgagt tctatacttc ctatactcct tatcagccgg aaataagcca aggtactctg
    23041 cagtatattt ttgaatacca aacattaatc accaacctga caggtatgga tgtagcaaat
    23101 gcttccctct atgacggggg tactgctata acagaagctg ccttaatggc atgctcctat
    23161 tcaaggaagg atgagattat agtttctgca ggtgtgaatc cagaagcaag acaaatactt
    23221 aaaacatatg ctcatgcaca gaatttaaaa gtaacagagg ttgaaacaaa ggacggggtt
    23281 actgatgccg ggatgctgga atcgctctta agcgataaaa cagcagccgt tatcgtacaa
    23341 aatcctaact tcttcggcct tcttgaagat ttaaaaggtt tggcagaaat aactcacaag
    23401 gttaagaagg ccagctttgt agcagcagta gagccaatat ctctggcact gctcaaaaaa
    23461 cccggtgaac tggacgtgga tattgttgtt ggcgaagggc agcctcttgg gatgcctctg
    23521 cagtttggcg gaccttattt aggtttcatg gctgtaaaga atgattacat cagaaagatt
    23581 cccggcagaa tcgttggaga aaccactgat ttagacggca aaaggtctta tgtgcttaca
    23641 ctgtcggcca gagaacagca tataaggaga gaaaaggcca cttcaaacat atgctccaac
    23701 caggggctga atgctctggc agccactgtt tatatggtga ccatgggcaa ggaaggattg
    23761 aaggaggtag ccgagcaatg tgtccaaaag tctcattatg ccttcaatga actgactaaa
    23821 tcaggcaagt acaggccgct gttcgataaa cccttcttta tggaatttgc cttaacctct
    23881 gaagacagcg ttgataaaat aaacaaggct ctcttggaag agaaaataat cggcggatac
    23941 gaccttggaa aggattatcc tcaatacaag aacgcttctc tatatgcagt cactgaaaag
    24001 aggacaaaag aagaaattga taaattaaga agagtattgg aggcggttaa ataatgaaag
    24061 aatacaataa attaatattt gaactatcaa aaccgggtag aaaagcatac agccttccag
    24121 ctcttgatgt tgaagaaaaa gcattagaca gtcttatacc ggctgaattt ttaactgata
    24181 aggggcttaa ttttcctgaa gtcagtgaag ttgatgttat aagacactat accaatcttt
    24241 caaataagaa ctatggtctg gacaaaggct tttacccgct gggatcttgt acaatgaagt
    24301 acaatccaaa gataaatgaa gatgtggtaa aatatgacgg gtttgcaaat gttcatccct
    24361 ttcaggcaga aagctgcatt caaggttcat tgcaggtaat gtatgaactt gaaagatccc
    24421 tgtgtgaaat aggcggtatg gacagcttca cactgcatcc ggcagcaggt gctcacggtg
    24481 agattacagg aataatgctg atcaaggcct accatgataa gaacggtgac accaagagaa
    24541 acaagataat tgtcccggat tcggctcatg gaactaaccc ggcaacagca gcaatggccg
    24601 gatattcaat agtagaaatc aaatcaaccg aaaatggtct ggtagacctt gaagcactga
    24661 aagcggcagt cggtgaagat acagcaggat taatgctgac aaatcccaat acattaggcc
    24721 tttatgacaa aggagtaaag gaaatatcaa gaatcgtcca tgaagcagga ggacttcttt
    24781 actatgacgg agctaacctg aatgcaatca tgggtcattc aaggcctgga gatatgggat
    24841 ttgacacagt tcacttcaat ctgcacaaaa cattctcaac acctcatggc ggcggcggtc
    24901 ctggatcagg tcccgtagga tgcaagaaga tactggctga attcctgccc aagcctttgg
    24961 taggcaagag atatgacggc acctacttcc tggattataa tgtaccgggt tcaataggca
    25021 aaattaaaga cttctacgga cacttcggaa ttttccttag agcgtatacc tacatattga
    25081 ttatgggtaa tgacggcctt aaaaaggcat ctgaaatggc tgtattaaat gccaactata
    25141 ttaaggaaaa gctaaaagat tactactacc tgcccataga cactttagtt aaacacgaag
    25201 ttgtattcgg cggattaaag gatactacaa gcggggttac aacgcttgat atagccaaga
    25261 gactcttaga ctacggatat catcctccta cagtttactt ccccttgatt attgaccagg
    25321 cattaatgat tgagcctacg gaatctgaaa gcaaagaaac aatcgatggc tttatagaag
    25381 caatgattgc cattgccaag gaagcaagtt cagatccgga actgttaaaa actgcccccc
    25441 atgacactct tgtaagaaga cccgatgaaa ctaaggcggc cagaaatccg gtagtaaaat
    25501 accaagggta gaaattaatt aatgacaaat taattattat gtgctagtat aagttaatat
    25561 tcagagtaga caagatgcgt taagtgttga gggatgggat gtcgccctta aacgaaggac
    25621 acaggtctgc ggtatcttgt cgcttccgct gtatttgaga aacttatgtt gctcctatat
    25681 tgtgcggagg tattactttg ctcaataagg aggtaaatat gagtagtaat actgagcgcc
    25741 acaaattcac atcattaaca attgtgagag cagggctttt tatatcaatg agccttgtgt
    25801 taaaggtatt gtttgaagta tatattcctt tggcaggaat tcccgccttg aggataaact
    25861 tcgccaatgt tccgctgatg ctttcgggta taattctagg accctttgca ggatttttaa
    25921 ccggagcggc agcagatata ataaacttta ttgcaaagcc ttcaggtccc tattttcccg
    25981 gattcacatt agcaagcgga ttatgcggat taattccggg aataattttc aagtatttaa
    26041 aaagagatat aaattacaac ctgctaaata caatttttat ctctttgctg tccgtgggtt
    26101 ttgtcctggt ctttatgcaa aacggcttgt tgacatttga caatggtata ttttatgacg
    26161 gtaaaccgct gaatgctgca tatgcagccg ggttcttctt gcttgtggcg atttatttga
    26221 taattcctgc taaggtgtca tcagcatcaa aagacctggc catgaataaa atagtgtttg
    26281 caatcagcgc aacccagctt attacatcaa taattttaaa cacttacttc ttatatgttc
    26341 tgtacggcat aggaataatg attactctgc cggcgagaat tttgacaaat ttctttatga
    26401 taccccttta ttcaataatt atcggtgcca tattgaatgc agtcaaagga agaatttagg
    26461 tgctcaggac tgcttgtgaa ggattaaaac atgtcagaaa cattaatgat tctgacatgt
    26521 ttttgtctta atccttcaag tgtttattaa ttgcatctgc tgccagctta gctgttgaaa
    26581 aggcagcctg gatgttaaac cctcctgtat ccccgtcgta gtccagcact tctccggtaa
    26641 aataaaggcc ggggattttt tttgattcca tggtctttga atttacttca gaagttgaaa
    26701 ctccgccttt tgtaaccatg gcaatattgt aatctccaag cttaatgatt ttcatgggat
    26761 gggctgaaaa catttgaatc aggctgttcc tctgaatttt cgaaagctgg ctgcagggca
    26821 gattttcatc aataccgcat atttcaagcg ccttttcaac aaaacgcctt ggtaaggcaa
    26881 ggtcctttaa tgctgttttt atgcttgtct ttgattgaat taaagattca aaaaatgacc
    26941 tgaagtcatc ttcgttacta aaggctgtga aattaatctt tattatgtct cccgcttcca
    27001 tgtatctgga attatttatt attacaggac ctgaaatatt tatgtgggta aataaaaaat
    27061 ctcctgcaaa ttcctttgtc tttttattgt ttttaaacaa ggataatttt atatttttaa
    27121 atgacactcc tgataattcg ccgaactggt aattttcaac gtagattggt gtaagtgcag
    27181 gcttaggttc aacaacactg tgccccaggg atgctgcaaa ttcataaccg gcgcctgtgg
    27241 agcctgtatc tggataggac tttcccccgg ttgctattat taaattataa gctgcatagg
    27301 cgaaggcatg agttttaata taaaacagct ttttatccgc atcataagac accctttcca
    27361 cacttgtatt taaaagcatt tgtgcatggt ttacgctgtc aattaataca ttcagcacat
    27421 ctatagcttt cagcgatgag ggaaatacct tgtcatcttc tcttataatg ctccttaaac
    27481 cccttttctt aaaaaagtca atggtctcct gattggtgaa attataaagg gcaggtttaa
    27541 taaacctgcc cttgtcaccg tatctgtcaa aaaattcatt aattgcacaa ttattagtat
    27601 agttgcattg tcctgccccg gtcagcaaaa gctttcttcc cggttctttg cttttttcaa
    27661 tgattaaaac ctttttattt tcaatatttg cagctgagaa taatcctgca ggtcctgcgc
    27721 ctataacaat aacatcataa atcattaaac tgtatcttcc atttcctctg tttctattat
    27781 ttcgcctgct tttggtattt catatgtttc catcatttta tccagagcaa atgcagcctt
    27841 tatttcatat tccacttcat tttcaacgta agctcctgta aacacaacag aatcattttc
    27901 caaattaaaa tcagtatact tattattgtc caggttaatt tgtttattac tgtcatttac
    27961 aaaatcaaac aaaacaaatt tgctgtcctt gtcttttagc aatattgtag ttgtgccttc
    28021 caataattgc atcttctgcg gctcgtcaaa ggacccgatg attttcttgt tttctatatc
    28081 taatatttta tttttgccgt cagaattttt aatcaaaatg taagagccca gccattcata
    28141 ggacccttcc ctgtcaaatg tggcaccatc gatcgctgca ggctggtttt caccttcgtc
    28201 ccagatgaat atctgactgt agtcatcacc gtatccctgg gttctgaata caatattttc
    28261 atcggttcct atgatttcaa tacctttaac actgtcatcg actaactctg agctttcctt
    28321 caagtatgat actatgctgt ctgcatccgt attttctgaa ttaaacttag gcatatcccc
    28381 gctttgaact attttcagcc tggattccgc ctcctccagc ttaaaaataa tattttcatt
    28441 gcttttagcc aagcttagat attcgtcttc caattcttcg aattttgttt taaattcatt
    28501 taactctgcc tttgttccgt ccagttcttc ttttaagctt aagttttctt tcttgatttc
    28561 ttctgttcct gcaatacctt catactcttc cgggcctaga ccagtctcct gtgatacagg
    28621 tatctgggtg cagcccattg caaataataa ggtaaaaaca attaaatata atctaaattt
    28681 tctcatggtc tcacatcctt tcgcttctta tgagggagaa attatcttta acttttactg
    28741 tatcattata cttgttgcct catattctcc gcccctttgg acagctatag ccacaatact
    28801 ttccccttct tccagatctt taaaatactt ggtttcacct gaagtatcgt atattatggt
    28861 cctgttggca actgtcaggt atataagctc tgttttccct gtacctgtta tactttgcat
    28921 cattatcagc cggtcatctt cattgataaa tatgatttta cctataaagc tcttggcagt
    28981 atccagctca gaaacttcca ttgaaactat ttcatctccc gaagtgttaa tgtatgcatt
    29041 atatcctaga cgcaagtcat aaattgatac attttttgtc ccgattgtaa tcttaacatt
    29101 gctgcttact gaatactgtt taatggctcc gtcttcgtca gcaattttaa ttttactttg
    29161 aggcgcaatt agtatttcct tgattacacc gctggcttca gcttctttgg ctatggtgtt
    29221 aatagcaatc atatcatcat attctgtggt tatggtaact tcatccccca ctctgacctg
    29281 gtcaaagctt gaatcatcat cattttttgt aacgtcaaca gaacttgtaa atataaatgt
    29341 ttttaacagg ccttcatccg ttttcacggt aacctttatg ggataattat aatcaatggc
    29401 tgtaatttct ccgtcaaaat atttaatgcg gcttactgaa ttaatcctgt aaacctggcc
    29461 gtcatccagc aaaagagtca cttcatcgct cttttcaagt cttcttaatt cagttgcctt
    29521 accgtcatga tatacatcaa cgtctgcttc tatttcaaag gtaagtttgt catcatcttc
    29581 atcgtaaata gttattgatg caggttctac atatgccaca tataaaatag tgcctcttac
    29641 aacatccttt gtgctgtcag cttcaatctc cagggcaagt ccctcttcgt taactctgca
    29701 gtcaacaagc atatcatttt ttaaatcaga caattctctt gttctgccgt ttatggttat
    29761 ttttgtgtca tcatttacac tgactttact ttctgctcct ctgtcatctt taacatatat
    29821 gtatgagtca gtcctctttg tttccacttc cgttattatg ccgcttactt ttgttgcagg
    29881 ctgatatttg tctaaatcag gcaatatatt gttgtcgctt atataatcaa atgcctttac
    29941 taacaaagta gcaatttcag cccttttaat ttcatcgacc ggattaatat tcccttcatt
    30001 gtcgccaaga attattttct tttcagacat aaggtagata tatggtcttg ctgaagcaga
    30061 tatttcagca ttgtctttaa aaggcaggac gtatgtcttc aggctttttg cttcatcctc
    30121 caaacccaaa gcctttgtca aaagcactgc cacctcttcc ctttttgcat cctggaaaat
    30181 atttttattt gcaaacatat ccttgacagc ctgctcgctt actatgccaa gcccaattgc
    30241 aaccgccaaa tagtccaatg accattcatt atacaaaaca ttttgcattc ttcctaaaga
    30301 aggcttgtat tcatcgatta tttctttttt tatatcatca tcaatagtat acagtctcga
    30361 cagcataata agggattcca gcacagttac gttgttgtcc ggcttgaatg taccatcttc
    30421 ataaccggaa acaagtccgt ctttttcaac tctttctatg tagcttctcg cccaatgatt
    30481 tgaaatatca ctaaagacta tcgcatttgc agtcattgta aacaacagag acaacacaac
    30541 agaaagtaaa ataattctgc ttattttttt cataatacct cccatatgtc ttttttttaa
    30601 taaattatat catatatatg ttgttactgc aataaaaatc gggacattcc ttggaccctc
    30661 cggctacccc ggaagggaga aggtgtgtct tttatataaa aaactattga cttacccgga
    30721 tattatgata aattttaatc ataatgatga ggcggattat ggcaggggta aattacagca
    30781 gatttaagct aaaaaattca atatttatga atattgccat aaatgaattt ataaaaagag
    30841 cagctgaaaa cttttatata ataaaatttg aaggagaaat atgggcatat tgataaaacc
    30901 ttccctgtta agaggtaaaa ttaccatccc tttctcgaaa agcctaagcc acagagcaat
    30961 tatatgtgct tctctttcca aggaagaaag caaaataaaa aacatagttt tttctgatga
    31021 tataagggct gtaattgagg gaatgaaaaa actgggggca gatataaggg aagcagatga
    31081 tttccttaca gttaagcggg ctgaaaacac cggagataat gctgtaaata atataattga
    31141 ctgcaaagaa tcagggtctg tcctgagatt tcttatacct ttgtctttgg tttttaaaga
    31201 taaatgcacc tttaccggaa aaggaagact ggttgaaaga ccacttgatg tatattatga
    31261 tatttttgaa aagtccaata tagaatatga aacaaacaag gggaaactgc cccttacggt
    31321 gaagggttcg ctgaagggcg gttattacag catacccggc aatataagct cacaatttat
    31381 cacaggacta ttttttgcac ttccccttct taaggataat tcggaaataa gaattacagg
    31441 ccctgtggaa tcaaaggctt atattgaatt gacaagagat gtaatgaaaa aatattgtgt
    31501 caatatagaa aaaatcgata gctgtaacta ctttataaag ggaggtcaaa aatataaggc
    31561 acataactgt gaaattgaag gggatttttc acaggcagcg ttcttccttg ctgcaaatgc
    31621 tttgggatcc aaggttgaat gccttggttt aaattatgat tccctccagg gagacaggga
    31681 aatattaaat ataattgaaa aatactcatt gccggaaaat gaaataacca tagatgcttc
    31741 acaaatcccg gacctggttc cggtaatatc agttcttgca tccttgaaag aaaactgcaa
    31801 aactactata atcaacgccg gcaggctgag gtacaaggag tccgacagac ttaaggcaat
    31861 agctgcagaa atgaaaaaac tgggggcttc aataaaggaa attgaaaatg gccttataat
    31921 tgaaggaaag aactctttgc acggaaatac aacggtttac agctggaatg accaccggat
    31981 tgcaatggct ttggctattg cggcaattaa atgcgatgat cccatccttt tggaaggtta
    32041 tgagtcggta aagaaatcat acccgaaatt ttgggatgat tataaaagct taggaggtgt
    32101 tataaatgag ctcaatgaca ggaaataaac tgaaaataag catatttggc gaatcccacg
    32161 gagaaggcat aggtgtagta atagacggcc tgccctcagg atttgctctt gattttgatt
    32221 acattaatca gcagatgaat aggcgacttc ccggaacaaa caccctgtcc accgaaagaa
    32281 aagaaaagga tgaatataaa atactcagcg gcttatttga aggaaaaaca acaggcactc
    32341 ctctgtctgc aataatttat aatattgaca aaaaatctga tgattatggc gaaagattca
    32401 aacttcgccc gggccacagc gattacacgg gatatatcaa atattcatca tataatgact
    32461 accggggggg aggccacttt tcagggagac ttacggcacc tcttttattt gcaggggctc
    32521 ttgccatgca gatattggaa agtaaaggaa tacttatagg gagccgcata aaaagcatat
    32581 ataatataaa tgatgatgat attgaaaata tggatgcaga tacgatcaaa aatttaagaa
    32641 gtatgagttt tcctgtcata tcaaaagaaa aaggcgctct gatgacaaaa aatatccttg
    32701 aacaaaagga aaaaggaaac tccctgggag gaatagtcga aacatttatt ataaatgttc
    32761 ctgcaggcta cggcgagcca ttttttgatt ctcttgaaag caagatggcg catatgatat
    32821 tttccatccc cggagtcaag ggaattgaat tcggtgcagg ctttgatatt acaaccctta
    32881 cgggaaaaga agcaaatgat gaattctatg tggccaatca taagataatt accgttacca
    32941 acaataacgg cggtgttttg ggaggaataa caaacggcat gcccatagtt attaaaactg
    33001 ccataaaacc cactccctca atatggcaac cacagaatac ggttgatata tcttcaatgg
    33061 aaaataccgt cgtaaatatt aaaggccgcc acgacccctg cattgttccc agggttgtag
    33121 cggttttaga aggagcctgc gcccttgtgc tccttgattt aattatggaa agggatggtg
    33181 aaaattggaa cttgataagg caagagcaga aatagacaag ctagataagg aaattgtgaa
    33241 gctgctggaa aaaagattca atttggcaag tgaaataggt ttcttaaaaa aagaacaaaa
    33301 ccttcctgtg tatgatgaat caagagaaag gcaggtcttg gaatactgcg caggtatatt
    33361 ggaaaataaa gagtattcaa aatacatctg cgatatttac tttcaaataa tgaacatctg
    33421 taaagacata cagaggttaa aggataagga atgaaaaaca tattaattat aggcatgtcc
    33481 ggcagcggga agacaaccgc gggcatgatg ctttcagaaa aattaaatat ggaatttgta
    33541 gatatggatg attacatagt atccgtggaa aataaaagca ttattgaaat gtttgaaata
    33601 agcgaggaat atttcagaaa tgctgaaagc aaatgctcaa agattttatc cggggaaaca
    33661 tctcttgtaa ttgctgccgg cggaggaatt gtcaaacgaa aagaaaatat ggactgtttt
    33721 gaaaaaaatt caatcatagt tttcttaaac agacctcccg aaaaaatcat agaagatata
    33781 gacataaaaa cccgccccct cctgaaagaa gggcgggata aagtttttac cctctacaat
    33841 gaaagaatcc atctgtatag gaaatactgc catattgaag ttttaaacga tacaaccctg
    33901 gacgacgccg tagatgaaat aataaatcgt gttaggacct gtttgggtta ggcataaagg
    33961 ctgtttttct ctccctttgg tcagaaaaat agggctatga cattaaggtc atctttctca
    34021 ccgtttcaaa ttcattttga atttcttccg aaggctcgtg agtcatttta gatactccat
    34081 aaatgaatat gcaggatatt ataaatgccg gaagaagctc atatacaccg aatattccgc
    34141 ccaatggctt taaaatcagc ttccaaacaa ataccattat acctccggaa agcatgcctg
    34201 caacagcccc ttccctgctt gttctcttcc agaacaggga gaatatgata attggtccga
    34261 aggtggctcc aaatcccgcc caggcaaagg atactattgt aaaaataaca ctgtcctcat
    34321 caagagctat gaatatacct attatacaaa ttactaccag gattattctt gaaacaatca
    34381 taacctgctt gtcggctgca tcctttttaa ttaaatgctg gtatatattt ttggaaaatg
    34441 ctgatgctgc aatcaataaa taagaatcag acgaacttat tgttgctgct aaaatccctg
    34501 ccattacaaa acctgcaata aaggcaggaa gtaaatttga tgacaataca ataaaaatac
    34561 tttccgagtc gctgtatgtc agcagggctg taggaaatac agctcttccg attattccta
    34621 tggctacggc agcacccaag gaaataacta cccagactgt tgcaattcgc ctggatgttt
    34681 ttagttcacc ggggtttctt attgccatga atcttaacaa tacctggggc atcccaaaat
    34741 acccaagccc ccatgcaagt gttgataaaa cagtgtacat accatactca cctgctgcat
    34801 tgtactgagg cactccattt acaacctgct gtacgccatc tgttacggcg ggtgatgcta
    34861 tgccgaaaaa ttcaaaaaat ccggggattt cttttgcatt ttcaataact tgtgcagttc
    34921 cacccgctgc ataagttcca acggctaaaa ttgtgactaa agcaataatc ataacaaatg
    34981 cctgcatggt atctgatgcg gattctgcca gaaatcctcc tataaaagtg taaaacaata
    35041 caaaagcggc tcccaatatc atcatatact gatatctgaa tccaaacagt gcattaaaaa
    35101 gcttacccac tgttacaaag cagctggaag cgtaaacaat aaagaaaacc agaataaata
    35161 ctgcagaaat cgtcattatt atttttttct tttctttaaa gcgattgctt aaaaaatcag
    35221 gaagcgttat ggaattgcct gccgcaaccg aatagttgtg aagccttttt gcaataaaca
    35281 gccagtttaa ataggttcct gcagctaagc ctattgcagt ccatacagca tcacttaaac
    35341 cataccagta tgcaacaccg ggaagtccca ttaaaagcca gccgctcata tcagatgctt
    35401 cggcactcat ggctgtaacc aacgggctga gtgttctacc tcctaaaaaa tagttttcac
    35461 tgctctcatt ggcacgcttt gcaaaataca atcctatgcc aataacaaca gccatgtaaa
    35521 taatcataga tgcaaaaatt tgtaatgttt ctccgctcat aatgtaaacc tccttcttta
    35581 aaattttatt tttatactat tcgctattta tttgggggcg aaaaaaacgc aggggagtgc
    35641 atgaaaatcg ggttagggca ttaaacaggc tgcatcacca atttgttcac tccctctggt
    35701 cgtgcaaatt gggatttcgc tgcatatgac ctaccagccg tttttctgaa aaaaacccag
    35761 aaaaacgggg ttagggcatt aaaaatccgc ctctaaaaag aggcggatgt ccgcggtacc
    35821 actctaactg cattatatac agtatctcgt taaaataagg gttttgccct ttgggttgct
    35881 gctcccaaac gctccgaggc gggttcaatc aacattggga caaaggtctt tcaccgggca
    35941 accttctctc taagtccatg catcactact attcctcttc acagctgaat attaatatta
    36001 aaaaacatac tataccaaga taaagatttt gtcaaggttt ttcttgaatt gttttgcagg
    36061 ttttttaatt ttcttgcatt acagagaaat ttatctccga tatatagcag ttatggctgc
    36121 taaattattt atcatttcat ctgttaaatc agttttcttc attctccagg tccagtttcc
    36181 gtttacagtt gccggtgtat tcattcttgc ttcatcacct aatccaagaa tatcctgcat
    36241 tgtagttatt gccaaaccgg cggctgagct ccatgcccct ctgataaatc cccaattcaa
    36301 tccctctttt gaatctaatt ttaaatactt ctttgcatag tccacatctt ctattcctgc
    36361 atttaaaaac catccttcaa cagttgagtt gtcatgggtt cctgtgtaga ctacgcagtt
    36421 cttgtcgtaa ttatggggta aatagtcact ttcctctctt gagtcaaagg caaactgcaa
    36481 taccttcatt ccgggaaaac ctgaatattt aagaagcttc ttgacatcct ctgttaaaaa
    36541 acctaaatct tcagcgatta tatttaattc tcccaattct tccttaatac tattaaaaaa
    36601 gtctgttccc ggccctttta cccaatgacc gattactgca tcttcaaagc cgtatttaat
    36661 ttcccaataa gcttcaaatc ccctgaaatg atcgattctt aatacgtcga aaatctcaaa
    36721 gctatgtttt attctgtttt tccaccatct atagttatct ttctccattt cagaccaatt
    36781 gtatatggga tttccccaaa gctggccctt acgggaaaat gcgtctggag gacagcctga
    36841 tactgtaatc ggcaaaagat cctcatctaa attaaatagg cgtggattgc tccatacatc
    36901 ctggctgtct tctgctacat atatgggcaa atctcctatg attctaacac ccttaacatt
    36961 ggcatatgat tttaacctat accattgctt atagaagaaa tactgagtaa atgtccaaaa
    37021 atatatcctg tcaatatttt caatttcaaa tttctctaca acttttgaat ccttcctcct
    37081 gtattcctcc tgccatatca gccaggaaac atttccatga tgttttttta ttgacataaa
    37141 aagtgcaaat tctttcagcc attcattatt atcccaataa aacctgtata aatcttcctt
    37201 taagtcttcc tttgcttttt cataagcctt ttccaagagg tgcattttgt tcctgtacag
    37261 cagtccgtaa tcaaccctgg tcacatcaca gcctaaatca atgctgttga tttcatcttt
    37321 agtcaggtat cccctctcaa taaattcatc cagttctata aaatacgggt tgcctgcata
    37381 tgaggaaaag gactggtagg gagaatctcc gtatcctgtt attcccaatg gcagtacctg
    37441 ccagtatttt tgttttgcat ctgccaaaaa gtctacaaag gaatatgcct ccttgccaaa
    37501 atctccaatt ccatattcgc cgggcaatga ggaaatatgc atcaatatac cactggacct
    37561 tttcctttcc atactttgct ccttcacaaa tccctgtaca gcagcgtgca caggataaac
    37621 aataaaaaaa taacagatta atcatatatt tattttaatg atttgtaaag gttttttgtt
    37681 ttaacatatt aaactgttga ttacctcttt tactgtggta attttattta catcgttcag
    37741 gtttgcaccg gaaaagacca agccgtccct tccttccaca gaatttatca agccttccga
    37801 tatgcagtat ggaatttccc tgggattgca gcccggcata catctataac atcttgtaag
    37861 aataaccttt cccgcacctt tgagaaaatt tgtttctatg gctcttccgg gcattcctac
    37921 cgggctttta ataattttta tattttcatc atcagcatta acatatgttg acttgaaatt
    37981 ttcgtgagcg tcacattcta tagtagggac aaacaagctt gctaactgaa aaccgtctgc
    38041 cccggcttct tcaagttcaa ggatatcctc cccggtcctt attcctcctg ctgcaatcaa
    38101 gggtatcttc actttgtatt cttcctcaaa ggcccttaat aaagacttta cttccttaac
    38161 aatatctttt aaaggcatgc atttattttc caaaagttca tccatcttaa aaccaaggtg
    38221 ccctcctgcc aatggtcctt ccactattat tgcatccggc agcctgtctg acttcagata
    38281 acttctgata attatttttg ctgccttggc agaagatact atgggtgcaa tcttcgtatt
    38341 tgtccccttt gttaactttg gaagagcaat tggaagccct gcacctgata cgataatatc
    38401 tattttttct tcaactgctt ttttaacata caattcataa ttgttcatag ctgccatgaa
    38461 attcatggca ataataccct ttgatttttc tcttgctttt ttaatctcct tgccgattgc
    38521 ccttaaattt gcttcaacag ggttatttaa aaaatctggc tctctaaagc cgatttgaac
    38581 accggatatg gttcctatgc ccccttcatt tgcaactgct gccgctaaat ttgcaagaga
    38641 tacgcctata cccatgcctc cctgaactat tgggattttt gcagtcaagt ctcctatttt
    38701 taattccttt aatatcatat gtccaccatt atttttatag tctattattt tgatattcaa
    38761 agtatatatt taaaaccatt ctatgtcaag aaaaaagtat tgccttggct ctattaatct
    38821 ttaatttctg caactgatag aaaataataa aaatatttag cgaacatttg caggacacga
    38881 ttataattct tagcgaatgc agactgaaaa tccgttaagc agcttgcact gtttgagcga
    38941 agcgagtttg caagctgtag gatttttagt cgtaataagc tcatagaatt attcgtgtcc
    39001 gaaaccgtga gcggatattt ttattatttt ctttgaaaaa agtaccgcct gagcagtact
    39061 ttttattcgt gtctgaataa tattttttta tttatttaca tattcgtcaa aagcatcctt
    39121 ggtaaactgc tgcttttctt cgattgcttc ttgaaccatc atgtccttaa ccttcattag
    39181 ccttgtttga ttacctcttt cgttttcatc aagcttcatt tgaatgtact tgatagtttc
    39241 ctcaagttgc ggaatcataa catattcaag agcattaact cttcttctgg tcttttcgat
    39301 ttcagctgca agaagttgaa cttctttttc aacagctgcc aatttta
//
LOCUS       DAOPUT010000005        38982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_972_length_38982_cov_18.042721, whole genome shotgun sequence.
ACCESSION   DAOPUT010000005 DAOPUT010000000
VERSION     DAOPUT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 38982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38982
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_972_length_38982_cov_18.042721"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1066
                     /gene="spoIIIAA"
                     /locus_tag="PKK75_01145"
     CDS             143..1066
                     /gene="spoIIIAA"
                     /locus_tag="PKK75_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HNZ82230.1"
                     /translation="MENFLEYLNLKIRNAVSDVFADMDEAIEIRLRINSPVLVKTIKK
                     DQFLDGNFIVNKKDIDEILGNLTGNSIHAYENEIKKGFITVEGGHRVGISGDLVYDKD
                     GIKGFKNITSLNMRIAREFPGCSKKVLNQIISPDKNIYNTLIIGPPLSGKTTFIRDVA
                     RNLSNGLKTPYFEGCDLTLIDERGEISAVYNGVPQMDTGRRTDVLSYCKKSDGFFMSI
                     RALSPRVIISDELGSVEDFEIIQYALKSGVKIIATAHGAGLDDVKRNIYLKDILENNF
                     FERAFILNKYPLKVNEVFDFQKNRVIYNDMD"
     gene            1053..1556
                     /locus_tag="PKK75_01150"
     CDS             1053..1556
                     /locus_tag="PKK75_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82231.1"
                     /translation="MIWIKAGLFAGTVVTIRYIFSLINNEGDEKIKKMKEILNFTEYL
                     RVYSCCMKMSIEEIYEKYNFKCLDTKLVIKSMIDFLEDKKSSKELMVYINKIMSTPFD
                     FNSHFTEIIDYYGYTYSDVLDRKLKLTIEGMEGSLKEFSTKHNEKKALNNKVSFLAGC
                     LAAIIMS"
     gene            1567..1764
                     /gene="spoIIIAC"
                     /locus_tag="PKK75_01155"
     CDS             1567..1764
                     /gene="spoIIIAC"
                     /locus_tag="PKK75_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HNZ82232.1"
                     /translation="MDIDIIFKVGAIGIVVAVFSQILTKTGRDEQAMLVTLAGVIVVM
                     ALIVSLMNDLFTEVKSVFNLY"
     gene            1778..2167
                     /locus_tag="PKK75_01160"
     CDS             1778..2167
                     /locus_tag="PKK75_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HNZ82233.1"
                     /translation="MLLVKIIFMAVITLITGITLSKFNSEFKVYITVVFGIVVIFLLF
                     SELRVYVEEIVDLFVRYDIKTEYFGTILKIVGIAYICDFISLLCKDLNYESVGKKVEI
                     AGKLIILIYSFDIIKVFLDQILVLVNG"
     gene            2172..3305
                     /locus_tag="PKK75_01165"
     CDS             2172..3305
                     /locus_tag="PKK75_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82234.1"
                     /translation="MKKITLLIIVITLVSTLKCYASTIDRAMDEMPTGDIEDYINEKS
                     AYFRENNIRIRDLLKNAFTGNLDLSIRDYAYYQLENEQGYFRDILKTAVNVFTICIVL
                     TIIGYFTDENTSKSVSDMVVLFSVIIIFTIILKDVLSIKSMLKSDFTDFKIITEEINA
                     IFLAAMLTFGKLSLLQFFQTSLNYTIGITTQFVYRFTDIMTVIMIAVILINNMNKLIN
                     AKLLYKALKKGTLLILSGFMIIVVINFSVQGYILYKTDNIFISSIKAMSPSSLPLVGN
                     AVTSFFGVFLKSLLMIKDVLGIVVIIFVLSSFGGSIIKISIALMAYKALGVITEPFNE
                     DISKFIYEMADMFYIYLICLVTPIIIVTVYYSIIINYMNNILG"
     gene            3308..3748
                     /locus_tag="PKK75_01170"
     CDS             3308..3748
                     /locus_tag="PKK75_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82235.1"
                     /translation="MKTTIINTLVLILIFNLIMIIFPEGKTQKFCRISIKLLIIIFVI
                     DNIFLNGSMNLKLLETAQAPAYEREVSVNSISPEIIDTINRENFQGEEVVSNISLSFT
                     KDMEMSAIVYVKRMLGNDETDKLKSNIAEIFQLNSDNIDIGRSL"
     gene            3745..4287
                     /locus_tag="PKK75_01175"
     CDS             3745..4287
                     /locus_tag="PKK75_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82236.1"
                     /translation="MNNFLKMLKENKKLSYAVNVFILLVIISLILKLDFSKDSQDSMS
                     YQESKVKTESYVYALEERMEEVLGKIDGINSVDVMIYTKRTPELQPVYDENSSYETNN
                     ETVSDGTKRNVNRETKQNKVILGGDNSVVERYYQYPEISGVLAVVKYSGNKDIYGILM
                     NSVKTLLDIKLNDIEIIVIN"
     gene            4298..4933
                     /locus_tag="PKK75_01180"
     CDS             4298..4933
                     /locus_tag="PKK75_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HNZ82237.1"
                     /translation="MIMRKETLVFLASLAIIFIIAYINMSLTPDNAFDDLETGQLAEE
                     DIAEIIEGGVNEVDFAEILPEETDFEILGDITDLELSEEASSEGVLGVLNANFANFKL
                     NKEKGNMDILDYLEGSLSSALISDSTKDQFEQLLLKKNSFIEAEQGIELMLQSKGYNE
                     SIAIVDESMVKVVTNDTIEQADATIILDVVVSETNYTPSQIKIVKFDNIDL"
     gene            4976..5377
                     /locus_tag="PKK75_01185"
     CDS             4976..5377
                     /locus_tag="PKK75_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNZ82238.1"
                     /translation="MDNEKFELGQVKISDDVVIIIAGIAASQVKGVNTARTGVTDGIT
                     NLFSKNNYAKGIKVEVTEETVVVDIFINVEYGYRINEVAKEVQLAIKKEIETMTDLQV
                     AAVNVHVLNIVDKEKDKDKIKDKEPVDIALE"
     gene            5529..5933
                     /gene="nusB"
                     /locus_tag="PKK75_01190"
     CDS             5529..5933
                     /gene="nusB"
                     /locus_tag="PKK75_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNZ82239.1"
                     /translation="MKRKETREEAVKIAYSMDINKELGKFNTAEYIDHFQLEEMDAEY
                     LDKTIVDMIDNLEIIDKYITDNSKDWKISRIAKVDLAVLRIALAEILYNETIPESVSI
                     NEAVEISKKFSNEDSHKFINGILGTVVRHIKK"
     gene            5930..6877
                     /locus_tag="PKK75_01195"
     CDS             5930..6877
                     /locus_tag="PKK75_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82240.1"
                     /translation="MTYILGIDTSAYTTSIALIDEENGEIIADERKLLEVKEGQKGLR
                     QQEAVFQHLKNLPDLYSKITQDLSKVKIVSVSSRPRNEEGSYMPVFMAGRNFGRVISH
                     TLNCGLIEYSHQENHISASLIQNYKNINEFLAVHISGGTTEFVHARKISKGFETKITG
                     GTKDITFGQLIDRLGIMMGFPFPCGRHMEEYIKGKTYDKVPKPKPAKGSYINLSGMEN
                     YYGGLLYKSTKEDIIASLFEYIAECIINIINSLSHLGLKTVIISGGVASNSHIRNSIL
                     KNLKDYEILLPAVDNSSDNALGLAFLPIIDRWHNEVKTY"
     gene            6858..8060
                     /gene="xseA"
                     /locus_tag="PKK75_01200"
     CDS             6858..8060
                     /gene="xseA"
                     /locus_tag="PKK75_01200"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNZ82241.1"
                     /translation="MKLKPIKVSDLNQYIKRYLMSNSILNNLRVEGEITNIRMSKTGY
                     TYFSLCDESSCISCVAFFADSISKNGDKIIAEGELSIYEAKGTCQLIVRNIERVGLGK
                     ILLDLELLKEKLKARGLFDRKRELPVFPQKIGIITSKTGAALKDILKTFESVKANIDI
                     TIYNSLVQGEKSKESIIEGIKYFNKAETDVILISRGGGSFEDLNVFNDLDLAMEVYKS
                     KVIVVTGIGHETDRTLTDYVADIYCHTPTAAAERIIRDYKDVEERLASLVYSLKSGTH
                     NAIALKNAELASVKYILKSSLPLENIYNAKAQLQAYKTLIKNETAKYINDHNLLLKIT
                     GEKLNSFDCSKQLKKGFALVSDNEGNIIKHREQVEIKDMLNIRFEEFMVEAETIDIKE
                     VWNGIRKL"
     gene            8041..8235
                     /gene="xseB"
                     /locus_tag="PKK75_01205"
     CDS             8041..8235
                     /gene="xseB"
                     /locus_tag="PKK75_01205"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNZ82242.1"
                     /translation="MEYESFESALDELKAIVEKFENKNDITIDELLVNYENGMKAYSY
                     CMKKLDDTQKKIKIIDEELT"
     gene            8527..9312
                     /locus_tag="PKK75_01210"
     CDS             8527..9312
                     /locus_tag="PKK75_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNZ82243.1"
                     /translation="MKNCRLDVYLHEKGYTDSREKSKQLITSSSVFVDKKLITKPSYQ
                     VNDDVEIEITREIFISRGYLKIEKAIQAFNINIENKIAADIGASTGGFTDYLLKHGAR
                     KVYAVDAGCGQLHDSLRNDIRVVNLENTNFRYIDVSVFIDDIDIVTVDVSFISLKYIL
                     PKVVEICHSNTDIIALVKPQFEAGKENIGKNGVVKDRKVHLEVLKNLAACCQINNLFL
                     LDIQHSPIKGAEGNIEYLAYIKAQGTTKEFNFKGLIDKAFEIL"
     gene            9332..9784
                     /locus_tag="PKK75_01215"
     CDS             9332..9784
                     /locus_tag="PKK75_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HNZ82244.1"
                     /translation="MKKYTRQTKILELISKSEVETQEELAEALKNMGIDVTQATISRD
                     IKELRLVKVMSKSGKYKYATIGQSQEGITDRLNKIFENSVVSIDNAMNMIIIKTIPGA
                     AQICASAIDYMGIDEIVGTIAGDDNVFVAVRDMAHVDSVLQEFKKNIR"
     gene            9795..11453
                     /gene="recN"
                     /locus_tag="PKK75_01220"
     CDS             9795..11453
                     /gene="recN"
                     /locus_tag="PKK75_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNZ82245.1"
                     /translation="MLLSLNISNFAVFENVSVSFAPDFNVFTGETGSGKSVLINAIEL
                     ILGDRASKELIRKDKDSALVEGIFDISGDDSLYRVLEKLNIEIDKDDFLIISRELLQS
                     GKSINKVNGRAVTLSTLKTIGSHLIDIYGQFGHESLFKKENHIKLLDSMIEKDLSLVF
                     KSYAELFSEYNRVQNEISILKTKLLNKDKKIEQLKYEIDEIDAAQLKENEEEELLKKI
                     KKLSNVQEIKKSLYEAISILNSDNINNVYTILNKAQKYDEKLLEYLSRIDIQLEELKL
                     LSYDLRDYSDNLSLDENELENIEKRMSLINRLKRKYGFSIEKINEYQNASQEELSLLL
                     EADSMLNSLIAQKDEIFRDLTQKAEIISEKRKDAAEYLEKQILVELNDLNMKNIEFKV
                     NMDKKKISLDGVDNVEFLISTNVGSDLNSVQKIVSGGEASRIMLAFKKIKSGAGQTMV
                     FDEIDIGISGRTAQMTGVKMNYIALNNQVICVTHSPQIASISKNNFLIEKKVVGNNTV
                     STVRKLNKEDKIYEIARLLSGMKITDKSLNNAVELIEFNSKISE"
     gene            11503..12576
                     /gene="spoIVB"
                     /locus_tag="PKK75_01225"
     CDS             11503..12576
                     /gene="spoIVB"
                     /locus_tag="PKK75_01225"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HNZ82246.1"
                     /translation="MKNFVKRVLFFIFFIAVLMTAVFFGFNLRTNPYKFAQNDKAVDT
                     DGRYIMPGGQTIGVELKTEGVLVVGLADIVNADNISTSPAKIGGIQIGDKILEIDSQK
                     LDAYEIIDYTMDKGVKQYIFKVEREGKIYNFTITPVQRYDSEEIKFGFWARDDIAGIG
                     TITFVEPETGTYSAIGHGISDSGTGNLIDIESGTISKANITSIKLGKKGEPGEIIGYI
                     LKDEESLGTVSDNTEFGIYGKINEDSMGYFSSDLLEIGKKEEIKLGPAYIISCVNDEI
                     KRYSIDITKIFYQNKADEKSFVIRITDEELLELTNGIIQGMSGSPIIQNNKIIGAVTH
                     VFMNDPSKGYGIYIEWVLDEIYK"
     gene            12795..13589
                     /gene="spo0A"
                     /locus_tag="PKK75_01230"
     CDS             12795..13589
                     /gene="spo0A"
                     /locus_tag="PKK75_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HNZ82247.1"
                     /translation="MQKKVKVVIADDNKEFRLILKDFLLSKKVFDIIDMAEDGLKALE
                     SVEKYEPDILILDIIMPQLDGLGVLERLHKKELKKFPKVIVLSAVGHDKVTQRAVNMG
                     VDYYIVKPFNFESFADRLLQIAELDTAKIQNKNKEIVYNDKMNTELSLEVKITEILHE
                     VGVPAHIKGYQYLRTSITDVVNNVELLGAITKELYPMIALKYSTTPSRVERAIRHAIE
                     VAWTRGKIETINNIFGYTIHNNKGKPTNSEFIAMISDKLRLEQKVS"
     gene            13687..14208
                     /locus_tag="PKK75_01235"
     CDS             13687..14208
                     /locus_tag="PKK75_01235"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ82248.1"
                     /translation="MNTEITVKSEKIFEGKILNLRVDTVELENQKYAKREIVDHKPAA
                     CILAVKNDNILLVRQYRKAVEEYIYELPAGILNIAEEPHEGALRELKEETGYKAGRIE
                     LIYEFYTSPGFSNEKVYLFKAYDLIFESTHFDEDECIETIEVNKDEAKKLLETGRIAD
                     SKTIIGLLHWLNY"
     gene            14307..14888
                     /locus_tag="PKK75_01240"
     CDS             14307..14888
                     /locus_tag="PKK75_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82249.1"
                     /translation="MNNTLKKLVKTENKIFLQLIMIFTGALILSALIYSLTGKGTMTS
                     INYDSISKMSFMQIFLTSFIRNSIYFLAVIFLTGIGQSSITKILFGAISLYYGLSIIY
                     IVRIVGMDFKYFMLTFTDYLIFFPILLYFTFISCSISKYTKKAKNIETISRKFDIIIT
                     GYIRISLVYLSIVTAYSFIYSFYVLILSRLMVR"
     gene            14888..15757
                     /locus_tag="PKK75_01245"
     CDS             14888..15757
                     /locus_tag="PKK75_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ82250.1"
                     /translation="MDITTYKTYLSQRNLSGNTISSYIYDIEKFSSYLDDDYDLGLLE
                     TKKAHILTYLVTLQKDGKSSSTISRTISALKNFFSFLKNEKLIMDNPAVSIHSPKQIR
                     RKPSILTEEEVARLMLLPEKSTFKGIRDLAMLELMYSSGIKVSELINIRIAQTNLNAG
                     IVSIGGDKERIIPLSKPAINAIDSYLNDFRDIKSAELNDFLFINISGEPISRQGIWKI
                     LKSYKKRMDLDKELSPQVLRNSFAVHMLSHGADLGTLQELMGLTSIEAAQNYIGNLEF
                     KSLEVFKRAFPRV"
     gene            15881..17050
                     /locus_tag="PKK75_01250"
     CDS             15881..17050
                     /locus_tag="PKK75_01250"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HNZ82251.1"
                     /translation="MRKVVLIVLDSVGAGELPDAADYGDEGSNTLKNIYRHAENFSLP
                     NLEKLGILNIDGFEDLKSSDSYLGSVAKCGQKSKGKDTTTGHWEISGLVLEKPFPTYP
                     EGFPKTLILEFEKRTGRKVIGNYPASGTEIIKELGRNHVETGNLIVYTSADSVFQIAA
                     HEDVVPLEELYSICRKAREMLKGEHGVGRVIARPFIGEDGNYTRTGNRKDFSLEPYRD
                     TMLDLLKNTGREVYAIGKIEDIFVNKGITRSNHTTNNEEGIEATIQAVKEDFEGLIFT
                     NLVDFDMVYGHRNNVQGYADALMYFDKKLHEIIAALNDEDVLIITADHGCDPTTESTD
                     HSREYIPLIFYGKNIKANNNLGILDSFASIGKTVLDIFDVENDLEGKSVKSRILG"
     gene            17119..18273
                     /locus_tag="PKK75_01255"
     CDS             17119..18273
                     /locus_tag="PKK75_01255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNZ82252.1"
                     /translation="MKKIITIFFVFIMLNMMIVPAYGIGPEEMQSRSAILINADDGSI
                     LFEKNAHDKMQPASITKIMLMVLISEKMDDGQITLDQEMTISENAAGMGGSQIYLEAY
                     ETQTVENMLKAISMRSANDASVAMAEFMYGSVEGCVKAMNDKAKELGMNDTVFTNVTG
                     LPDPDHLTTANDIAVMTRELLKYNYMNVYMLTWMDSVYVGKEKDSEQVLVNTNRLINN
                     YDGLLAGKTGYTTEAKYCLSAAAKRNDTTLIAVVLGCDNTKIRFNEVSKLLNEGFANY
                     KNIIFHKMGETITTAPVYCGKQETINVVSRENINYFTESNCKVEDFNLEYVIDENLKA
                     PLARDTQIGRVILSKDGQVLGEFELYPETNIVKENLFKFFIQNVLKTTIR"
     gene            18286..19011
                     /locus_tag="PKK75_01260"
     CDS             18286..19011
                     /locus_tag="PKK75_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNZ82253.1"
                     /translation="MDYSVNINVFQGPFDLLFHLIEKKEIDIYDIPIAEITDQYLDYL
                     EEILQFNMNIASEFIFMAATLIEIKSQMLLPKKEKEEDPRQELVNKLLEYKMFKDISE
                     ELKKYEDESSYYFFKPREEMALTSDTRTEQLYLNEINVYELYNMYLSLIKKQNFKIDQ
                     EEKYKVYRETYRVKDCMEDLAKKIKKFGKVSLFSTFKEKGSVSKEYIVTLFLAILELT
                     NNKGIRLYQDNKYGDIIITQERK"
     gene            19008..19544
                     /gene="scpB"
                     /locus_tag="PKK75_01265"
     CDS             19008..19544
                     /gene="scpB"
                     /locus_tag="PKK75_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNZ82254.1"
                     /translation="MNTKSVLESLMFAWGEPLNISEISKILDMPPQSITHVLDEMIKE
                     VNNNEDRGLIIQRFGSSYQLTTKKENYEYIQSLLESTVNKSLTTAAMETLSIIAYKQP
                     VTKVEIERIRGVKCSQVVKGLLDKGLVKEAGRLDKPGRPVIFATTDEFLRHFGISSID
                     ELPKFKGDPDDKDDMETA"
     gene            19665..20039
                     /locus_tag="PKK75_01270"
     CDS             19665..20039
                     /locus_tag="PKK75_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82255.1"
                     /translation="MGILIVLAGGLLFLYLNIKCLFKINFTFYFIRLYFYVIIFRKKH
                     EYERKININFAKKLIDRFKSPEARDKAENRLKYIRRIRKGFNVFYIKDIFFYPECIME
                     KQSFALEFVVVNRMLKRSLLNG"
     gene            20147..20527
                     /locus_tag="PKK75_01275"
     CDS             20147..20527
                     /locus_tag="PKK75_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82256.1"
                     /translation="MSKDIESIIKTTLENINNLTENEKLISSKILYDNTNYLAISKLN
                     MNFIIGGSDIDKKFRTVDLKPFAGAAYVNLQLNPQILIYETPDNDVKTININNETTAG
                     DLTTSIMNIMSFIKEVREKKDCDI"
     gene            20517..21551
                     /locus_tag="PKK75_01280"
     CDS             20517..21551
                     /locus_tag="PKK75_01280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNZ82257.1"
                     /translation="MIFKRIISTILIILFFTCQFSQIEAMPGISAQAYIVIDGQSGRI
                     LNSHNAEIKLPIASTTKIMTAILAIETIEDLEQKIEVPPDCTGIEGSSIYLRPGQTVS
                     VIDLLYGTMLRSGNDAAATLSKFAGRNDPEGFVSLMNKKAKELGALNTNFVNPSGLHD
                     DNHYSTAYDLALISAYAMKNDTFKKIASAEKYKAVSMNNMFYNKNKVVYQYKHGTGIK
                     IGFTKAAGRCLVASAEKDGTEIIAVLLNDNNWFNESYQIFDWAFENYKSYSVVEKGQF
                     VKYTPERNPVFIGDSFKCSFTDEEIKQIRFEPLIEPELMSNDTETKTAGTYNIYLGKT
                     KIHSGTLITE"
     gene            21645..22355
                     /locus_tag="PKK75_01285"
     CDS             21645..22355
                     /locus_tag="PKK75_01285"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ82258.1"
                     /translation="MAEERLQKYMARCGITSRRKAEELILQGSVKVNGIIEKELGSKI
                     NPEKDEVTVYDKIIYEKKEHIYIKLYKPEGYITTVKDQFNRKTVLDLVNIKDRIYPVG
                     RLDYDTSGLLLLTDDGDLANKLMHPKFRIYKTYEAEVSGTISEESINLLRTGLIIDGY
                     KTAPARVKIMEQKGNNTSVQISIHEGKNRQVRKMFEAAGKKVIKLKRISFGDIVLGGL
                     KPGQWKYLTDEEVSFLKR"
     gene            22358..22930
                     /locus_tag="PKK75_01290"
     CDS             22358..22930
                     /locus_tag="PKK75_01290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ82259.1"
                     /translation="MDKKYGKIAEMVHFLIKASYEKRENLNFLDLTCGNGHDTLFLSN
                     LAGKTGSVTAFDIQNAAIERTNKLLEANGQFNNYTIIKDSHEFFERYLTGNIDAAVYN
                     LGYLPDYSKEIFTKPETTISSVVQLLPYLKNWGKIYITAYITHDKGYEINAIAEFLKT
                     LNKKEYNVIHINVINKENSPPELFIIEKKA"
     assembly_gap    22931..23030
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23054..23251
                     /locus_tag="PKK75_01295"
     CDS             23054..23251
                     /locus_tag="PKK75_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ82260.1"
                     /translation="MADEKVLYQNIPWCKGCGVCVGFCPAKVLTIKNGKCYIAHRDKC
                     IFCGQCELRCPDNAIYIGGKR"
     gene            23248..24393
                     /locus_tag="PKK75_01300"
     CDS             23248..24393
                     /locus_tag="PKK75_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNZ82261.1"
                     /translation="MNKKRYKLVQGNEACVEGAIAAGMRFYAGYPITPSTEIAEISSQ
                     KLPKLGGKFIQMEDEISSIAAIIGASLTGLKSMTATSGPGFSLMQENIGYAAITEIPC
                     VIVNVQRGGPSTGLPTSPAQGDVMQARWGTHGDHPAIALTPNSVQETFEYTVKAFNLA
                     EKYRTPVILLMDETVGHMREKMAYKNISEIEIINRKKPTCKPEDYVAYRPDEDMIPPM
                     ASFGEGYRYHVTGLSHDETGFPTNSNEVSEKLIKRLVDKVEKNIDDFSYFEEYKLEDA
                     EIVIIAYGGVSRSAKSAVDELRKRNIKAGLFRPITIWPFLEKQINKVSLHAKEIYVAE
                     MNLGQYSLEVERAACKNCIVKGINRINGELITPEEIIEAIGGESNEL"
     gene            24383..25198
                     /locus_tag="PKK75_01305"
     CDS             24383..25198
                     /locus_tag="PKK75_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNZ82262.1"
                     /translation="MNCNQTTKEYYRVNRLPHIWCPGCGHGTLTNTVVRAIEKLGLNK
                     DEVVIVSGIGCSSRAPGYLDFNTLHTTHGRALAFATGIKLANPKLHVIVIMGDGDSSA
                     IGGNHLIHAARRNIDITTIVFNNNIYGMTGGQSSPTTPFGDFATTAAYGNIDKPFDLC
                     SLAQGAGATYVGRATAYHAVLMQKLVEKGIRNKGFSFIEGLSLCPTYYGRNNRKGNPL
                     KMHTFFKEHCIDKKLVDKDPQKGIFKILIGEFYNNPQPEYIESYQVIIDMLKE"
     gene            25208..25756
                     /locus_tag="PKK75_01310"
     CDS             25208..25756
                     /locus_tag="PKK75_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNZ82263.1"
                     /translation="MKKHEIRLSGSGGQGVILASIILADAAIEQGLNAIQTQSYGPEA
                     RGGASKAEVIISNEEINYPKINNSGILLSLTQGAYDKYIEYLDKDGILIVDDYVKLSG
                     KDTAKVYKLPILKNSQEKFETSMVANIISLGAIYELIGEDTIDKNIMIQSITERVPPM
                     TTQKNIEAFEEGRRLVRGYYEQ"
     gene            25746..26603
                     /locus_tag="PKK75_01315"
     CDS             25746..26603
                     /locus_tag="PKK75_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNZ82264.1"
                     /translation="MNSDLMSKIKNNFHKFSKGQKLIAQFIIEHYDKAAFMTAAKIAD
                     RVDVSESTVVRFASSLGYSGYPEMQKALQVLIKNKLTAVQRIGLDADVNDKLKIHKKI
                     LKNEMNNMRFLFEHFDMGALDRATELILNANRVFVLGLRTSSTLSNYLGFYLDVILDN
                     VKVLNNSGVNSLYEEIIRVKDNDLMIIISYPRYSKTTIDAAKFVKEKGVKIVAITDTE
                     ESPAYSLADASLLGKSNIVSFVDSLVVPMTIINNLIINISLKEKEEIVEYYNILEKLW
                     DNNSMYQNF"
     gene            26908..27588
                     /gene="cmk"
                     /locus_tag="PKK75_01320"
     CDS             26908..27588
                     /gene="cmk"
                     /locus_tag="PKK75_01320"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNZ82265.1"
                     /translation="MVHGLRKENKMIIAIDGPSGAGKSTVARLLSKELGFEYIDTGAM
                     YRALAYKAYKKNLVINEAEIEEMLKTTNISYYDNKIFLDGENVENKIREETISIAASK
                     VSALKIVREKMVEIQRKIAADKNAVLEGRDIGTIVFPDADYKFFITASLEERAKRRYA
                     QLIAGNINAEYENVLRDMIDRDENDSKRQFSPLKPAEDAIIIDTTKMDLSEVTKTIAG
                     YIGGNNVL"
     gene            27578..28174
                     /locus_tag="PKK75_01325"
     CDS             27578..28174
                     /locus_tag="PKK75_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ82266.1"
                     /translation="MFYKIAVFVCRIIAFIIFDFKVYNKENLDNTKGGLIVCGNHRCM
                     IDPVMLAISTRRQIHYMGKKELFESKFWSYIFRSLGAFPVDRKGVSLSSIKSSLNILN
                     SGGVLGIYPEGTRVKTGYDEKNAKSGIALIANKANVKILPVYLDGPYKFRGKLRLYIG
                     KEKDYFENYTGKLNSDKYTEIGIEILKDIYKLKDEGIK"
     gene            28171..30726
                     /gene="ispH"
                     /locus_tag="PKK75_01330"
     CDS             28171..30726
                     /gene="ispH"
                     /locus_tag="PKK75_01330"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNZ82267.1"
                     /translation="MKIFISEYNGFCGGVKQAVLKADRILSEESKLYCYGEIIHNKDV
                     VDKFKEKGMVIVDEPPDDNDAKLLIRSHGVGKDIYDILEEKNIEIVDATCVKVKKIHK
                     IVEEFKNNGYNIIIVGDKDHPEVQGIAGWCNKEADIIKGPGELEKIIDLSKKYCLVCQ
                     TTFNLNIFEEILKTIEINNFSNIIVHDTICDATRKRQEACMALASKCTVMLIVGSKNS
                     SNTKKLYELSKELCENTFLIQNYKEIPYNYIDKNTIVGVSAGASAPDWVIEEVIEMLE
                     NLNDNMIEKNEQAEIAEQSEDLEKPELLGEAKTAEQAEPAEEAEPVEAAEQAEPAEEA
                     EPVESAEQAEPAEEAEPVEAAEQAEPVQPEAEEKQEQVQETKQTEKKDSMHELLENYG
                     GVVYIRTGEKVKGTVIYVSPDAVSVNINYKSDGIITRDEFSMTEVADLREMVKEGDEI
                     EAVVVKISDQDGNVVLTRKPLEEQKIWDDLEQQRIRGEIIEIPIVEASQYGIYGKLNG
                     IKGFIPRNQISIKRDVDPSEYVGKNLKVKILETKNKKGRKQLVLTSRSVEKIEKELKD
                     KEVWETIQEGEVYEGTVKNLQDYGAFVEINGIDGLLHINEIAWTRIKHPSEVLKPGDK
                     IDVKVKSIDRENKRLSLSYKATVKSPWAKFLDKYQKGDTVTGLVTRIVDFGAFVKVDD
                     IECLLHIKDLDWARTEKVTDVLQEGQEVTAKILSITRKDRKVGLGLKQLTEHPFDVYA
                     KTLNKDDIIQVEVTRILLDGIHVNANGNIDCFIPIAKVSNEKLRTPAQVVKVGEIKDA
                     KVQSIDMKGKNLNLTFILDDKGEEITEKITYQPEDANFTIGESIGDALDDLLK"
     assembly_gap    30763..30862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            31052..32092
                     /locus_tag="PKK75_01335"
     CDS             31052..32092
                     /locus_tag="PKK75_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ82268.1"
                     /translation="MLKEFLFDVCSNHFVSGFEHINGDILTKYFKPYTDTLEKDKLGS
                     YIFKRTGTRDTKIMLAAHVDEIGLMITKILEGGFLSFTTVGGINPASLVAQDVTIYGK
                     ENIFGVIGIKPPHITSEEERTKPHKIKDLFIDTGYTKEKLESLVKIGDIATVNREPLS
                     LQGNVISAKAFDDRACVAVLLETAKELSKISHKSNVYFTATAQEETGMRGATTSSYKI
                     APDIGIVLDVGFGKTPDMPPDAPELGKGPIVAYGGRMNAKLTKKFVETCKKYNYPLQH
                     EAVPARTGTDTEALHISRDGIPCILLSIPLRYMHTSVETIDLYDVEITGKAIARFINE
                     LDNSDLEEVLCF"
     gene            32083..33099
                     /locus_tag="PKK75_01340"
     CDS             32083..33099
                     /locus_tag="PKK75_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNZ82269.1"
                     /translation="MFLKELTELSGVSGCEYEVRNFIKDKLSQMGCDFYVDKLGNIIA
                     HNKGRKNKTIMVAAHMDEVGLIVSNIDSEGFIKFKAVGGIDQRVLNSKAVLVGENKIP
                     GVIGSKAVHLMTSQEKESSLPIDKLYIDIGTNSKEETEKLVNIGDYVSFNSSYVEFGD
                     NLIKAKALDDRTGCSIILELMNQKLDADFYGVFTVMEEVGLRGAETAAYQLEPDLGIV
                     LEGTVCADMPEVEDRDKSTLIGKGAALSLMDATTVYDIDIVRSVAKIAEGNNIMYQYR
                     TSASGGNDAGAVHKTKSGAKAVAISTPCRYIHSSVNVASKKDIESVYMLAKQVILENQ
                     KGEQ"
     gene            33099..34082
                     /locus_tag="PKK75_01345"
     CDS             33099..34082
                     /locus_tag="PKK75_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNZ82270.1"
                     /translation="MMFNSELMKRLSDCFGPSGREKMIREMIKNEIKDYADEITTDPL
                     GNLIARKKGTGKKILFSAHMDQIGLIITHVDEKGFLRFANVGGLNAKELLGLRMVFGN
                     GLEGVICQEELKDKEKLTTGKLFLDVASTDKEFIKKNFRTGDMCVFKSEYYETKDCVI
                     CKAADDRIGCYVLIEALQNHPETDNDVYYVFSVQEEVGCRGAKTAGYSIDPELAIALD
                     VTATGDSPDGIKMDVKLGGGAAIKLKDNSLITHSDVKDLLTHLAEKNNIKYQYEILEF
                     GGTDAGQIHISREGVPSGVISIPSRNLHSSGEIFNKADVEECIKLVIAAAE"
     gene            34373..36355
                     /locus_tag="PKK75_01350"
     CDS             34373..36355
                     /locus_tag="PKK75_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ82271.1"
                     /translation="MDKSKEIDMEAVGLEWEKMINNQPVDENVVSSIILKSWHRSALA
                     GVNPYTVDENLYIKAEDLVQYYDSTKKVIDDEFRSILHKIADEFDLSFLLYDNKCKIV
                     SVLAVSKNYKTIGYSGSEDIVGTNSICLALQYDTPMQVFGKEHYCKIHHNYNCSAAPI
                     HDLDNNIIGAINIGGIISHKHTLETLGLAQSIAKIIEDKIQINQMVAQLTISNQTLNN
                     IIEYDSSGIIYFVGNEIISYNKSLLNMFEIMSTDNLIIVHESIHNILNQLKEHLHHDE
                     IENIEIVLNINNEKKSFITSIKQFSIDENKAACRLYKFDSTNKYMRLNINNRAVYTFD
                     DIIGECENMVRLKELAKKTARTSSSILICGESGTGKELFAHSIHNESFRKNKPFVAIN
                     CGAIPSELIESELFGYEPGAFTGASNKIKFGKLEIASGGTFFFDEIDSMPLKVQIKLL
                     RALSSNRITRIGGIKEIPIDIRIIAATKKDLLEEIELGQFREDLYFRINIINLTIPPL
                     RDRKDDIPILAESFINYNEKHSNIPDVKVDPEFYEALKYYFWRGNVRELRNVIEKAVL
                     LNDKETLTINNLPREIVSAYKYKSLKSKLSANIIETRDDKNLLKTGEEIIIEFVLNEE
                     NWNLTNTAKRLGISRPTLYRKIEESPKLSKRKKMLQ"
     gene            36737..38611
                     /locus_tag="PKK75_01355"
     CDS             36737..38611
                     /locus_tag="PKK75_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNZ82272.1"
                     /translation="MLYGYAGKMLFVNLSNDEIKEEILTKELAEKFIGGYGFGAKILY
                     EMMPAGADPLGPENILGFLTGPTTGTKSFFSGRYTIVHKSPVTGGWNDANSGGYFGPE
                     LKKAGYDAIFINGISEKPVYIYINDGKVEIRDASHLWGMDTKKVWSVLKEETKEQRLR
                     ISAIGPAGERLSYISCPINDGHRAPGRGGGGAVMGSKNLKAIAVRGTGEIPVKNPEKL
                     LEINKKISAVMKESQTAYAFRTWGTGVGTPASALSGDSPVKNWGGVGVLDFEDDKAQK
                     VGAIALDRYKTKKYSCANCPLGCGAEYKVEDGRWPIEQTERPEYETAAAFGSTCLCSE
                     EDALLMCNEICNIYGIDTISAGMTVAWAMECYNEGVLSKEELDGIDLTWGNGEAMVAL
                     LQKMADGDGCGKILANGSQYAAKYWGKGQEYLQTASGIELPMHDPRFAPGLARTYQFD
                     PTPGRHVKGGIGSLQMGGNELTDKYVYTDTGMMDLVLTTNTEIMNSSGLCYFHALVSP
                     PGVQNEFISAITGMEFDDKESLKTGQRIYLMRHAFNVREGLKPSDFKLTRRAVGKPPL
                     TAGPLQGVTVDEELLGKNFFESAGIDFETGKPSLELLQKLGGLDEVIADMYVKAGETV
                     "
     gene            38615..38851
                     /locus_tag="PKK75_01360"
     CDS             38615..38851
                     /locus_tag="PKK75_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNZ82273.1"
                     /translation="MKIKMKYFGLITNDLGSEAELPEGSSVSDLLNHIVKQDPNYKEI
                     LTRATILVNNSKVDIDTVLKHNDEVMVLAVLGGG"
BASE COUNT        14416 a         5932 c         7941 g        10493 t
ORIGIN      
        1 aataatttga cgaaatgctc tttatgacct aaccaatttc tttgaccacc atccctttgt
       61 tacaaaagaa tgtccccaat tagtggcata aatttataaa aggataatat tatagattaa
      121 gaggacaagg cagggggtac aaatggagaa ttttttggaa tatttaaact taaagattag
      181 aaatgccgtt tccgatgttt ttgccgatat ggatgaagca attgaaataa ggcttagaat
      241 caacagtccg gtacttgtta aaactataaa aaaagaccag tttctagatg ggaattttat
      301 tgtaaacaaa aaggatattg acgaaatcct ggggaattta accgggaatt caatacatgc
      361 atatgaaaac gaaataaaaa aaggctttat tactgtagaa gggggacaca gagtcggtat
      421 aagcggagat ttagtttatg acaaagacgg aataaaggga ttcaaaaaca taacatctct
      481 taacatgaga atagccagag aatttcccgg atgctcaaaa aaagtcttaa atcagataat
      541 aagtcccgat aaaaacattt acaacacctt gattataggg ccgccccttt caggcaagac
      601 aacatttata agggatgtgg caaggaatct gagcaacgga cttaaaactc cgtattttga
      661 gggatgcgac ctgactttaa ttgatgaaag aggagaaata agcgctgttt ataacggggt
      721 tccccaaatg gatacaggca gaaggaccga tgtattatcc tactgtaaaa aaagcgacgg
      781 ttttttcatg agcataagag ctctttctcc aagagttata atttcagatg aattagggtc
      841 tgttgaggat tttgaaatca ttcagtatgc gttgaagagc ggggtgaaaa ttatagcaac
      901 agcacatgga gccggtttgg atgatgtaaa aagaaacata tatttaaaag atattcttga
      961 aaataatttc tttgaaagag cattcattct gaacaaatat cctttaaagg ttaatgaagt
     1021 gtttgatttt caaaagaaca gggtgattta caatgatatg gattaaagca ggactttttg
     1081 caggaacagt agttactata aggtatatat tcagtttaat aaacaatgaa ggtgacgaaa
     1141 aaataaaaaa aatgaaggaa atattaaatt tcacggaata tctgagagtc tactcctgct
     1201 gcatgaaaat gtccattgaa gaaatttatg aaaaatataa tttcaaatgc ctggatacaa
     1261 aattggtcat taaatcaatg attgactttt tggaagataa aaaaagctct aaagagctaa
     1321 tggtttacat aaataaaatt atgtcaactc cttttgactt taattcgcat tttacagaaa
     1381 taattgatta ctatggctat acatattcgg atgtattgga cagaaagctt aaacttacca
     1441 ttgaaggaat ggaaggatct ttaaaagaat tttctaccaa acacaatgag aaaaaagctt
     1501 taaataacaa agtatcattt cttgcgggct gcctggcagc tatcataatg tcataggagg
     1561 actcatatgg atatagatat aattttcaag gtgggggcaa taggaatagt agttgcagta
     1621 ttcagtcaaa ttttaacaaa gacgggaagg gatgaacaag ccatgcttgt gacattagcc
     1681 ggtgtaattg ttgtgatggc tttgattgtc agtttgatga atgatttgtt cacggaggta
     1741 aaatctgtat tcaacttata ttgatgagga aaaaaatatg ctgctggtga aaattatttt
     1801 tatggctgta attactttaa ttacgggaat aaccttatcc aaattcaatt ctgaattcaa
     1861 agtatatata acagttgttt tcggtattgt tgtaatcttc ctgcttttta gcgagcttag
     1921 agtatatgtg gaagaaatag tggatttatt tgtaagatat gatataaaga ctgaatattt
     1981 cggcactatt ctaaaaattg taggcattgc atacatttgt gatttcatat ccctcttatg
     2041 caaggacctg aattatgaat ctgtgggtaa aaaggttgaa attgccggta agcttataat
     2101 actgatatat tcctttgata ttataaaggt attcctggac caaattctgg tcttggttaa
     2161 cgggtgatga aatgaaaaaa ataacattgt taataattgt gataaccctg gtatctactc
     2221 taaaatgtta tgcctcaaca attgaccggg caatggatga gatgcctaca ggcgatattg
     2281 aagattatat aaacgaaaaa agcgcatatt tcagagaaaa caacataagg atacgggacc
     2341 tgctaaaaaa tgcatttaca gggaatttgg accttagcat aagggattat gcctattacc
     2401 agctggagaa cgagcagggt tatttcaggg atattttaaa aaccgccgta aatgtgttta
     2461 ccatttgtat cgttcttaca ataatcggtt attttacaga cgaaaacaca agtaaaagtg
     2521 tttcggacat ggttgtactc ttttctgtaa ttattatttt tacgattatt ctcaaggatg
     2581 tactgagtat taaaagtatg ctgaaaagtg attttaccga ctttaaaatt ataactgaag
     2641 agattaatgc aatatttctg gcggccatgc tcacattcgg caaattgagc ctgctgcagt
     2701 tctttcagac atccctgaac tacacgatag gcatcaccac acagtttgtc tacaggttta
     2761 cagacattat gactgtaata atgatagccg taattttgat aaacaacatg aacaagctta
     2821 ttaacgccaa gcttttgtac aaggctttga aaaagggaac ccttttaatt ttatccggct
     2881 ttatgattat tgtagtcata aatttttctg tacaaggata tatattatat aaaacagata
     2941 atatttttat aagctccata aaggcaatgt cgccttcatc actgcctctt gtgggaaatg
     3001 cggtaacatc attcttcggg gtatttttaa aaagcctctt gatgataaaa gatgtactgg
     3061 ggattgtagt aataattttt gtcctgtcat cattcggggg atccataata aaaatcagca
     3121 ttgcactgat ggcatacaag gcgctggggg taattaccga gccttttaat gaagatatat
     3181 caaagttcat atatgaaatg gcagatatgt tctatattta tctgatatgt cttgtaaccc
     3241 cgataataat cgtcacggtt tactactcca taataataaa ttatatgaat aacatactgg
     3301 gataattatg aaaacaacaa ttataaacac attggtacta atactgatat tcaatttaat
     3361 aatgataata ttccctgaag gcaaaacaca gaaattttgc agaatatcca ttaaactttt
     3421 aatcattatt ttcgttattg ataatatatt tcttaatggg agcatgaatt taaagctgct
     3481 ggaaacggca caagctcctg catatgagcg ggaagtgagc gtaaacagca tttctcccga
     3541 aattattgat acgatcaaca gggaaaattt ccagggagaa gaagtggttt caaatatcag
     3601 ccttagcttt acaaaagaca tggaaatgtc agcaatagtg tatgtaaaaa ggatgcttgg
     3661 aaatgatgag acggataaac ttaaaagtaa catagctgaa atttttcaac ttaattcaga
     3721 taacatagat atagggagga gcttatgaat aactttttaa aaatgctgaa agaaaacaaa
     3781 aaattaagct atgcagtcaa tgttttcata ttgctggtga taatatcctt aatattgaaa
     3841 cttgattttt ctaaagattc acaagattct atgtcttatc aggaaagtaa agtgaaaaca
     3901 gaaagctatg tatatgcttt agaagaaaga atggaggaag tacttgggaa aatagacggt
     3961 attaatagtg ttgacgtaat gatttacact aaaagaacac ctgaactgca gccggtttat
     4021 gacgaaaaca gcagttatga aacaaataac gaaaccgtga gcgacggtac aaaaagaaat
     4081 gtgaacaggg agacaaaaca aaacaaagta atattgggag gggataattc agttgttgag
     4141 cggtattacc aatacccaga aatatccgga gtgcttgcag tggtgaaata cagcggaaat
     4201 aaagatattt acggtattct tatgaattct gtaaaaactt tattggatat caaattaaat
     4261 gatatagaaa taatagtgat taactaaggg ggttattatg ataatgagaa aagaaacatt
     4321 ggtattttta gcatctctgg caataatatt tattatcgcc tatataaata tgagcttgac
     4381 gccggataat gcatttgatg accttgaaac aggccagctg gcggaggaag acattgcaga
     4441 aattattgaa ggcggtgtaa atgaagttga tttcgctgaa atattgccgg aggaaacaga
     4501 ctttgaaatc ctaggcgata ttaccgattt ggagctttca gaggaagctt cctccgaagg
     4561 cgtacttgga gttttaaatg caaattttgc caacttcaag ctgaacaagg aaaagggcaa
     4621 catggatata ttggactatc tggaagggag cttaagctca gccttgatat ccgacagcac
     4681 caaggaccag tttgaacagc tgttacttaa aaagaactca tttattgaag cagagcaggg
     4741 aattgaactc atgcttcaat caaaaggtta caatgaatca attgccattg tagatgaaag
     4801 tatggtaaaa gttgtaacta atgatacgat tgaacaggca gatgcaacaa taattcttga
     4861 tgtggtggta tcagagacaa attatacacc ttctcaaata aaaattgtga aatttgacaa
     4921 tattgatttg taaaaaattt caacttctgc tataattatt ttaggaggtg ctattatgga
     4981 taatgaaaaa tttgaattag gccaggtgaa aatatccgat gatgttgtaa taatcatcgc
     5041 aggcattgca gcaagccagg ttaaaggcgt caatactgct cgcacaggtg tgaccgacgg
     5101 tattaccaac ctattctcga aaaacaatta tgcaaaaggt ataaaggttg aagtgactga
     5161 agaaacagtg gtagttgata tttttatcaa tgttgaatac ggttatagaa taaacgaggt
     5221 tgcaaaggaa gttcaactag ccataaagaa agaaattgaa acaatgacag acctgcaagt
     5281 agcagccgta aatgtccatg tacttaatat agttgacaag gaaaaggata aagacaaaat
     5341 aaaagataaa gaacctgttg atatagcctt agagtaaaat gaatacatat gtggaagggg
     5401 acacagtacg gggacattcc ttcatggcct tgctttaaat gcttactaac catgaaggtg
     5461 tgtccccctt cctgcagtgt ccctctctga cgctttaccc atcagcagtc acagtgccgg
     5521 agaaaaaaat gaaacgtaaa gaaacaagag aagaagcagt taaaatagca tacagcatgg
     5581 atataaacaa agagctgggc aaatttaata cagcagaata catagaccat ttccagcttg
     5641 aagaaatgga tgcagagtat ttggataaaa caatagttga catgattgac aatttagaaa
     5701 tcatagacaa atacattacc gacaattcaa aggactggaa aataagccgc attgccaaag
     5761 ttgaccttgc agtcttgcgg atagctcttg ctgaaattct atataatgaa acaatacctg
     5821 aaagtgtgtc aattaatgag gcagtggaaa taagcaaaaa attttcaaat gaagattcac
     5881 ataaatttat caacggaatt ctcggaactg ttgtaaggca tataaagaaa tgacatacat
     5941 tcttggaatt gataccagcg cttacactac atccattgcc ttgattgatg aagaaaacgg
     6001 tgaaattatt gcagatgaga ggaaactgct tgaagttaag gaaggacaga aaggtttgag
     6061 acagcaggag gcagtttttc agcatttaaa aaacctgcct gatctttatt ctaaaattac
     6121 acaggacctg tcaaaggtta aaattgtatc tgtaagttca aggcctcgaa atgaggaagg
     6181 ttcctatatg cccgtattca tggcaggcag gaacttcggg cgggtaattt cccatacatt
     6241 aaactgtggg ttaattgaat attctcatca ggaaaaccat atctctgcct cattgatcca
     6301 aaactataaa aatatcaatg aatttttagc agtccacatt tccggaggaa caacggaatt
     6361 tgttcatgca aggaaaattt caaaaggttt tgaaacaaaa attacaggag gaacaaagga
     6421 tatcacattc ggtcagctta tagacaggct tggaatcatg atgggatttc cctttccctg
     6481 cggcaggcat atggaggaat acattaaggg gaagacttat gacaaagtcc ctaagccaaa
     6541 accagccaag ggctcatata tcaacttgtc aggcatggaa aactactacg gcggcttgct
     6601 ttacaaaagt acaaaagaag atattatagc ttcacttttt gaatatatag cagaatgcat
     6661 aataaatata ataaacagct tgtcacatct tggacttaag accgtgatta tttcaggcgg
     6721 ggttgcttcc aacagccata taagaaacag cattttaaaa aatttaaagg attatgaaat
     6781 attactcccg gctgtggaca attcttccga taatgcttta ggtcttgcat ttctgcctat
     6841 tattgacagg tggcacaatg aagttaaaac ctattaaagt ttctgacttg aaccagtata
     6901 taaaaaggta tttaatgagc aattcaattc tcaataatct gagagtggag ggcgaaatta
     6961 caaacataag aatgagcaag acaggttata catatttttc attatgtgat gaatcatcct
     7021 gtatatcttg tgttgctttt tttgctgact caatttcaaa aaacggcgac aaaataattg
     7081 cagaagggga gctttccatt tatgaagcca aggggacctg ccagctgatt gtcagaaata
     7141 ttgaaagggt gggattaggg aaaattcttc ttgatctgga gcttttaaaa gaaaagttaa
     7201 aagcaagggg attgtttgac agaaagagag aacttcctgt tttcccgcaa aaaataggca
     7261 taattacatc aaagacaggt gcagccttga aggatatact aaaaaccttt gaaagcgtga
     7321 aagcaaatat agatataact atctacaaca gcctggtcca gggagaaaaa tccaaagaaa
     7381 gcataattga aggaataaaa tattttaaca aggctgaaac tgatgttatc cttatttcaa
     7441 gaggcggagg cagctttgag gatttaaatg tttttaacga tttggacctg gcaatggaag
     7501 tttataaatc aaaggttatt gtagttacag gaatcggaca cgaaacagac agaacattaa
     7561 ccgactatgt tgccgatata tactgtcata ctcccacggc agcagcagaa agaatcataa
     7621 gagactacaa ggatgttgag gaaaggcttg caagtcttgt atattcatta aaatccggaa
     7681 cacacaatgc catagcctta aaaaatgcag aactcgcttc tgttaaatat attttaaaaa
     7741 gcagcctgcc cttggagaat atttacaatg caaaggcaca gcttcaagcc tataaaacat
     7801 tgataaaaaa tgaaacagca aaatatatta atgaccataa tttgttgcta aaaataaccg
     7861 gcgaaaagtt aaattcattt gattgcagca aacagttaaa aaaaggattt gcactggtat
     7921 cagataatga ggggaatatt ataaaacaca gggaacaggt ggaaataaag gatatgctga
     7981 atataagatt tgaagaattc atggtggaag cggaaactat cgatataaaa gaggtgtgga
     8041 atggaatacg aaagctttga aagtgccttg gatgaattaa aagccatcgt agaaaagttt
     8101 gaaaacaaaa atgatatcac tattgacgag ctgcttgtca attatgaaaa tggaatgaag
     8161 gcttattcct attgcatgaa aaaattggac gacactcaaa agaaaatcaa aataatagat
     8221 gaagagttga cctaaaccaa tttcttcgac cgatagggag aaagaaactg ttggtgggtc
     8281 attcgcaacg agttcccgct ttatgcgacc ggagggacca attgaattgg tgaacgagac
     8341 tgctggatgc tataatttat gcgattttag gagcaaaata acggtaaccg ggaagtcaca
     8401 aacagaaaat taataattat caaaaatatt aattgatata aataaattct tatgctataa
     8461 tttcattgaa ttattcaaat atctgcatta ataatagaat atttatgtat taggcaaggc
     8521 gtaaggatga aaaattgcag gcttgatgtg tatttgcatg aaaaaggtta tacagacagc
     8581 agggaaaagt caaaacagct tattacgagc agttcagtat tcgttgataa aaagctgata
     8641 acaaaaccgt catatcaggt aaatgatgat gttgaaattg aaatcacaag agaaatcttt
     8701 ataagcaggg gctatttaaa aattgaaaaa gcaattcaag cattcaacat aaatattgaa
     8761 aataaaatcg cagcggatat tggagcttca accggcggat ttacagacta tcttctaaaa
     8821 catggagcaa gaaaggtata tgcagtagat gcgggctgcg gtcagcttca tgattcattg
     8881 agaaatgata ttcgggttgt taatttggaa aacacaaatt tcagatacat tgacgtatca
     8941 gtatttattg atgatataga cattgtaact gttgatgttt catttatttc actgaaatat
     9001 attttaccca aggttgtcga aatttgtcat agcaatactg atattatcgc attggtcaag
     9061 ccgcaatttg aagcaggaaa agaaaacata ggcaaaaatg gagtagtcaa ggacaggaag
     9121 gttcacctgg aggtcctaaa aaatctagcc gcctgctgtc aaataaacaa tttatttttg
     9181 ctggacatcc agcattcacc aataaaagga gcagaaggca acatagaata tctcgcctat
     9241 ataaaagctc agggaactac aaaagaattt aattttaaag gcctgataga taaagccttt
     9301 gaaatattat aaaaataaca agaggtggca aatgaaaaag tacacaagac aaacaaaaat
     9361 tttagagttg atttcaaaat ctgaagtgga aactcaggaa gaactggcag aagcccttaa
     9421 aaacatgggt attgacgtta cccaggctac aatatcaaga gatattaaag aactcagact
     9481 ggtcaaggta atgtccaaga gcggcaagta caaatatgca acaatcggtc aaagccagga
     9541 aggaataacg gacagattga ataaaatatt cgaaaattcc gtagtttcaa ttgataatgc
     9601 aatgaacatg ataataatca aaacaatacc gggagctgcc caaatatgtg cgtcagccat
     9661 cgattacatg ggaattgatg aaattgtagg taccatagca ggtgacgaca atgtttttgt
     9721 agccgtaagg gatatggcgc atgtagattc agtattgcag gaatttaaaa agaatataag
     9781 ataatggtga taatatgctg cttagtttaa atataagcaa ttttgcagta tttgaaaatg
     9841 tatccgttag ttttgcccca gactttaatg tttttacggg agaaacagga tccgggaaat
     9901 ccgtattaat caatgcaatt gaactgatat tgggagacag ggcttcaaag gaattaatca
     9961 gaaaggataa agattcggcg cttgttgagg gaatttttga tatttccggc gatgacagcc
    10021 tttatagagt tttagaaaaa ctgaacatag aaattgacaa agatgatttt ctgataatat
    10081 caagggagct tttacaaagc ggcaaaagca taaacaaggt caacggaaga gctgtcactt
    10141 taagcacact taaaacaata ggcagccatt taattgacat ttacggtcaa ttcggccatg
    10201 aaagcttgtt taagaaagaa aatcacataa aacttcttga cagcatgata gaaaaggacc
    10261 tgtccttggt atttaaatcc tatgctgagt tgttttctga atataaccgg gttcaaaatg
    10321 aaataagcat tctcaaaaca aaactcctga acaaagataa aaaaattgag cagctaaaat
    10381 atgaaatcga tgaaatcgat gcagcccagc tgaaagaaaa tgaggaagaa gagcttttaa
    10441 agaagattaa aaagctttca aatgtgcagg aaataaaaaa gtccctttat gaggcaatta
    10501 gcattctaaa cagcgacaat ataaacaatg tttacaccat tttaaataaa gctcaaaagt
    10561 atgatgaaaa gctcctggaa tatctttcaa gaattgacat tcaattagag gaattaaaac
    10621 tcttaagcta tgatttaaga gattattcag ataatttgtc ccttgatgaa aatgaacttg
    10681 aaaatattga aaagcgaatg tctcttatta atcgtttaaa aagaaagtac ggtttttcaa
    10741 ttgaaaaaat aaatgaatat caaaatgcgt cacaggaaga attaagcctg cttcttgaag
    10801 cagattcaat gcttaattct ttaattgctc aaaaggatga aatattcaga gatttgaccc
    10861 aaaaagcaga aattatatct gagaaaagaa aagacgcggc agagtatctt gaaaaacaga
    10921 ttttagtgga attaaatgac ttaaatatga aaaatattga gttcaaggtc aacatggata
    10981 agaagaaaat atcccttgac ggtgttgaca atgttgaatt tttaatatcc accaatgttg
    11041 gaagcgactt gaattctgta cagaaaatag tttccggagg ggaagcgtca agaatcatgc
    11101 tggcctttaa aaaaataaaa agcggcgcag gccagacaat ggtttttgat gaaattgaca
    11161 ttggaataag cggcaggact gcccagatga ccggtgtgaa aatgaattac attgctctta
    11221 acaatcaggt aatatgtgta acacattctc cccagattgc atcaatttca aaaaataact
    11281 ttttaataga aaaaaaggta gttggcaata atactgtatc cacagtgaga aaattaaaca
    11341 aggaagataa aatttatgaa attgccagat tattgagcgg tatgaaaata actgacaaat
    11401 ctttaaataa tgctgtggaa ttgatagaat ttaacagtaa aataagtgaa tagaacaagt
    11461 ttcatatgga aaagttaagg ataaaactat taggagaaca taatgaaaaa tttcgtaaaa
    11521 agagttttat tctttatttt ttttattgcg gtattgatga cagccgtttt ttttggtttt
    11581 aacctaagaa caaatccata taaatttgca caaaatgaca aggcagttga cactgacgga
    11641 agatatataa tgcctggggg ccagaccata ggtgttgagc ttaaaaccga gggggtactg
    11701 gttgtaggcc tggctgacat agtcaatgcc gataacatat caacatctcc tgcaaaaatc
    11761 gggggaatac aaatcgggga taaaatactt gagatagatt cacaaaaact tgatgcttat
    11821 gaaataatag attatacaat ggataaaggg gttaaacaat atatcttcaa agtggagagg
    11881 gaaggtaaaa tatacaattt cacaattact cccgttcaaa gatacgacag cgaagaaatc
    11941 aaattcgggt tctgggccag ggatgacata gcaggaatag gaacaattac atttgtagaa
    12001 cctgaaacag gaacctacag cgcaatagga cacggaattt ctgattctgg aacaggcaat
    12061 ttgatagaca ttgaatccgg aaccatttca aaagcgaata ttaccagcat taagctgggc
    12121 aaaaaaggcg agccgggaga aattataggt tatattttaa aagatgagga aagcctgggt
    12181 acggtatcag ataatacgga atttggaatt tacggcaaaa tcaatgaaga cagcatggga
    12241 tacttcagtt ctgatttatt ggaaatcggg aaaaaagaag aaataaagct tggacctgca
    12301 tatataatct cttgtgtcaa tgacgaaata aaaagatact ccattgatat aacaaaaatc
    12361 ttctatcaaa ataaagctga tgaaaaaagc tttgtgataa gaattacgga tgaagagctt
    12421 ttggaattaa ccaacggaat tatccaggga atgagcggaa gccccataat acaaaacaat
    12481 aaaataatcg gcgctgtaac acatgtattt atgaatgacc cctcaaaagg atatggaatc
    12541 tatattgaat gggtgcttga tgaaatatat aaatgattaa atttattata tttataatac
    12601 aattttaatt aaaaataagt catatttttt atcgacaaaa tttttacata ttatgggaaa
    12661 tatggtatat actattctta tataacctat atatttacaa aatatgaatt aaaaaataat
    12721 tatattcagg aaaagagtat tttttaattc ttttgcgtat tatttgtggg aataaaagta
    12781 ttgggggaag agagatgcaa aaaaaagtaa aagttgtcat tgctgacgac aataaggaat
    12841 tccggttaat tttaaaggac tttttactca gcaagaaagt ctttgacatc atagatatgg
    12901 cggaagatgg actcaaagca ttggagtctg tcgaaaaata tgaacccgac attcttatcc
    12961 tggacataat tatgccacag cttgacggtc ttggcgtact ggaaagactg cacaaaaaag
    13021 aactgaaaaa atttcccaag gtgatagtgt tatctgccgt aggccatgac aaggtaacac
    13081 aaagggctgt caatatgggt gttgattatt acattgtaaa gcctttcaac tttgaatcat
    13141 ttgcggacag acttcttcaa atagccgagc tggatactgc aaagatacaa aacaagaata
    13201 aggaaatcgt ttataatgat aaaatgaaca cagaactcag cctggaagtt aaaattacgg
    13261 aaattctgca tgaagtaggc gtgcccgctc atattaaagg ttaccagtat ctaagaactt
    13321 caataactga tgttgtaaac aatgttgaac tgctgggggc cataacaaag gaattatatc
    13381 caatgattgc attaaagtac tcaactactc caagcagggt tgaaagagcc atacgccatg
    13441 ccattgaggt tgcatggacc cgggggaaaa ttgaaacaat caacaatatt ttcggttata
    13501 ctatacataa caacaaaggc aaacctacca attcggaatt tattgcaatg atttcagata
    13561 aactccgcct ggagcaaaaa gtttcataag ttaaatttac ttgacaaaag catttatact
    13621 aattagaata agtccattaa ttaatcttgc agggatattg cacgagttta gttttaggag
    13681 gaagtattga atactgaaat tacggttaaa agcgaaaaaa tatttgaagg taaaatactt
    13741 aatttaagag tagatactgt cgaactggaa aatcagaaat atgccaaaag ggaaattgtg
    13801 gaccacaagc ctgccgcttg tattttagct gttaaaaacg acaatatact tttagtcaga
    13861 caatacagaa aggctgttga agagtatata tacgagctgc cggcaggaat tctaaatatt
    13921 gcagaggagc ctcatgaagg ggctttgcgc gaattaaaag aagagaccgg atacaaagca
    13981 ggtagaatcg aactgattta cgaattttat acatcgccgg gtttttcaaa tgaaaaggta
    14041 tatttgttca aagcttatga cctgatattt gaaagtactc attttgatga agatgaatgt
    14101 attgaaacaa tagaagtgaa taaggatgag gcaaaaaagt tgctggaaac cggcaggata
    14161 gctgacagta aaacaataat aggacttctt cactggctga attactagat ttcatgcaat
    14221 gaattaaacc tactaatgaa ttaaacctct tgtacaggca tatattatag aaagcttagc
    14281 aggaggtttt ttattgggag gtttatatga acaatacact aaagaaactg gtgaagactg
    14341 aaaataaaat tttcttacag ctgataatga tttttacggg ggctctgatt ttatcggcat
    14401 tgatttattc gttgacaggg aaaggaacaa tgacgtcaat taattatgac agcataagca
    14461 aaatgtcttt tatgcagatt ttccttacgt catttataag aaacagtatt tactttttag
    14521 cggtaatatt tttaaccggt atagggcaaa gcagtataac aaaaattctt ttcggagcca
    14581 tttctctata ttacggtctg tcaattattt acattgtcag gattgttgga atggacttca
    14641 aatacttcat gcttactttt actgattatt taatattttt tcccatttta ctttatttta
    14701 catttatttc atgttcaatt tctaaatata ccaaaaaagc aaaaaatata gagactattt
    14761 ctagaaaatt tgatataatc ataactggat atattagaat ttctttagtc tatttatcca
    14821 ttgttacagc ttattctttt atttacagct tttatgtatt aatactaagc agattgatgg
    14881 tgagataatg gatattacaa cttataaaac atatttaagc caaagaaact taagcggcaa
    14941 tacaatcagc tcctatatat atgatattga aaaattcagt tcatatcttg acgatgatta
    15001 tgaccttggt ttgcttgaaa caaaaaaagc gcatatactg acatatcttg tgaccctgca
    15061 gaaggacggc aaaagctcat caacaatatc aaggaccata tcagcattaa agaatttttt
    15121 ttcatttcta aaaaatgaaa agctgattat ggacaaccct gcagtaagca ttcacagccc
    15181 caaacaaata agaaggaaac cttcgattct tactgaagaa gaagtagcca gacttatgct
    15241 tcttccggag aaaagtacgt tcaagggtat aagagacctt gctatgctgg agcttatgta
    15301 ttcatcgggt atcaaagttt ccgagcttat aaatataagg attgcccaaa caaatttaaa
    15361 cgcaggaata gtctctatcg gaggggacaa agaaagaata attcctctca gcaagcctgc
    15421 aataaatgca attgacagct atttaaatga tttcagggat ataaaatctg ccgagctcaa
    15481 tgattttttg tttatcaata tttccggcga gcccatttca agacagggta tatggaagat
    15541 tttgaaatcc tataagaaaa gaatggattt agataaagaa ttatctcccc aggttttaag
    15601 aaattcattt gcagtacata tgctttctca cggggctgat ttgggaacac tgcaggaact
    15661 catggggctc accagcatag aagctgcaca aaattacata ggcaatctgg aatttaaatc
    15721 actggaagta tttaaaagag cattcccccg tgtatgacac aatccgtttc acactaataa
    15781 tgatgtttga gaccatgtaa ttaatggtat aataggagta taaaaatagt aaagggataa
    15841 atattatccc ttaatttatt atgaatagga gaagcacatg ataagaaaag tagttttgat
    15901 agtattagac agcgtgggag caggagagct tccagatgca gccgattacg gggatgaagg
    15961 aagcaacaca ctcaaaaata tatacaggca tgcagaaaat ttttcgctgc cgaatttaga
    16021 aaaattagga atactgaata ttgacggatt cgaagacttg aaaagctcag acagttattt
    16081 gggctctgtg gcaaaatgcg gacaaaaatc aaaggggaag gatacaacca cgggccactg
    16141 ggaaatctcc ggtcttgtac ttgaaaaacc attccccaca tatcctgagg gctttcccaa
    16201 gacccttatt ttagaatttg aaaaaagaac agggagaaag gttataggaa actatcctgc
    16261 atcaggtaca gaaataatta aggagcttgg caggaaccat gttgaaacgg gaaatttaat
    16321 agtttacaca tctgccgaca gtgtttttca aatagccgct catgaggatg tggttcctct
    16381 ggaggagctc tacagcattt gcaggaaagc aagagaaatg cttaagggtg agcatggagt
    16441 aggaagagtt attgcaaggc catttatcgg agaagacggc aattatacaa gaaccgggaa
    16501 cagaaaggat ttttccctgg aaccttacag ggatacaatg cttgaccttt taaaaaacac
    16561 cggcagggaa gtgtatgcca tagggaaaat agaagatatt tttgtaaata aggggatcac
    16621 aagatcaaat cataccacaa acaatgaaga aggaatagaa gcaacaatac aagctgtaaa
    16681 ggaagatttt gagggtttga tttttacaaa ccttgtggac tttgacatgg tttacggcca
    16741 tagaaacaat gttcagggat atgctgatgc attgatgtat tttgataaaa aacttcatga
    16801 aataattgct gccttaaatg atgaagatgt tttgattatc actgcagacc acggctgcga
    16861 ccccacaact gaaagcaccg accactcaag agaatatatt cctctcatct tttacggaaa
    16921 gaatatcaaa gcaaacaata atcttggcat acttgacagc tttgcatcca tcggcaagac
    16981 ggttcttgat atttttgatg ttgaaaatga tcttgaagga aagagtgtta aaagtagaat
    17041 tttaggatga caaaaggtca gtttataatg tatgaatcaa caaaatatgg ttattctaca
    17101 taaaaaggag gtagttttat gaagaaaatt ataaccatat tttttgtatt tattatgtta
    17161 aatatgatga ttgtacctgc atacggaatt gggcccgagg aaatgcagtc ccgttctgcc
    17221 attctgatta atgcagacga cggcagtatt ctatttgaaa aaaatgctca tgacaaaatg
    17281 cagcccgcaa gcattacaaa aataatgctt atggttttga tttcagaaaa aatggatgac
    17341 ggtcaaatta ctttggacca ggaaatgact atatcggaaa atgcagcagg catgggagga
    17401 agccagatct acctggaagc atatgaaact caaacagtgg agaacatgct taaagccata
    17461 tccatgcggt cagccaacga tgcttcggtt gccatggcag aattcatgta cggttcagta
    17521 gaaggctgtg ttaaggcaat gaacgacaaa gcaaaggaac taggaatgaa tgacaccgta
    17581 tttacaaatg ttacaggcct gcctgacccg gaccacctta caacagccaa tgatatagct
    17641 gttatgacca gggagctttt gaaatataat tatatgaatg tatatatgct tacctggatg
    17701 gattcggtgt atgtgggcaa ggaaaaggac tcagagcagg tacttgtgaa tacaaacagg
    17761 cttatcaata attatgacgg cttattggca ggcaagacag gctataccac tgaagcaaaa
    17821 tattgtttgt ctgcagctgc aaaacgaaat gacactacat taattgcagt ggttttagga
    17881 tgcgacaata caaaaattcg atttaatgaa gtatcaaagc ttttaaatga aggatttgca
    17941 aactataaaa atataatttt ccataagatg ggggagacta tcactacagc accggtttat
    18001 tgcgggaagc aggaaacaat aaacgtggta tccagggaaa atataaacta ttttacagaa
    18061 agtaattgca aggttgaaga ttttaattta gaatatgtaa tagatgaaaa tcttaaggct
    18121 ccattagcaa gggatacaca aataggaagg gttatactgt caaaggacgg gcaggtattg
    18181 ggcgaatttg aattgtatcc ggaaacaaat attgtaaagg aaaacctctt taaatttttt
    18241 atccaaaatg tattgaaaac caccataaga taaggaaggc ttgaaatgga ttattccgta
    18301 aatataaacg tgttccaggg accctttgac ctcttgtttc accttataga gaaaaaagaa
    18361 atagatattt atgatattcc tatagctgaa ataactgatc aatatctgga ttacctggaa
    18421 gaaatccttc agtttaacat gaatattgca agcgagttta tatttatggc tgcaacactg
    18481 attgaaataa aatctcaaat gcttcttcct aagaaggaaa aagaggaaga ccccaggcag
    18541 gaactggtta ataagctgtt ggaatacaaa atgtttaaag atatatcaga agaattgaaa
    18601 aagtatgaag acgagagctc ttactacttt ttcaagccca gggaggaaat ggccctaaca
    18661 tcagacacaa ggaccgaaca gctttattta aacgaaataa atgtatacga actctacaat
    18721 atgtacttat cgcttataaa aaaacaaaac tttaaaattg accaggagga aaagtacaaa
    18781 gtatacagag aaacctatcg tgtaaaggat tgcatggaag accttgcaaa aaaaataaag
    18841 aaatttggaa aagtttcatt attcagtact ttcaaagaaa aaggaagcgt atcaaaagaa
    18901 tatattgtta ccttgttctt agccatattg gagcttacaa acaataaagg aataagatta
    18961 tatcaggata ataaatacgg cgatataata atcacgcagg agagaaaatg aatacgaaaa
    19021 gtgttttgga aagcttgatg tttgcctggg gagagccctt gaacataagc gaaatatcaa
    19081 aaattttaga tatgcctcct caaagcataa cacatgtttt agatgaaatg ataaaagaag
    19141 taaataataa tgaagacaga ggccttatta ttcaaaggtt tggcagttcc taccagctta
    19201 ccaccaaaaa agaaaactat gaatatattc agagcctcct ggaaagcacg gtaaataaaa
    19261 gtctgactac agcagcaatg gagacactgt caataattgc ctacaagcag cctgttacaa
    19321 aggtggaaat agagagaatc aggggggtta aatgctccca ggtagtaaag gggcttcttg
    19381 acaaggggct cgtaaaagag gcgggaaggc ttgataagcc cggaagaccg gtgatttttg
    19441 caacaacaga tgaattcttg agacatttcg gcataagctc aatagacgaa ttaccaaaat
    19501 tcaagggtga tccggatgat aaagatgata tggagactgc ataacaaggg gacgggtgta
    19561 ctgtaatata tgacaagatg acgtgtctgc cataaaatac gtggcgctat gtggccggct
    19621 gatgacaaga cggattgtac tcaggccaag gagcgatata tagcatggga atattaatag
    19681 ttcttgcggg gggactctta ttcttatatt tgaatatcaa gtgtttgttt aaaataaatt
    19741 tcacctttta ttttatccgc ctatattttt atgtgattat ttttagaaaa aaacatgaat
    19801 atgaaagaaa aataaatatt aattttgcaa aaaaattaat agacaggttt aaatctcctg
    19861 aagcaaggga taaggcagaa aaccggctga aatatatcag gcgtataaga aaaggattca
    19921 atgtattcta cattaaagat atctttttct atcctgaatg cataatggaa aaacaatcct
    19981 ttgctcttga atttgtggta gtgaacagaa tgctcaagag atcgctgcta aatggataat
    20041 tttttgataa gtatctttct gcctgcaggc atgaggatac tttttttgtt ataattctca
    20101 atttttaggg catacttaat atgataaaag cgagaaaggg gaaaacatga gcaaggacat
    20161 agaaagcata atcaaaacaa ccttggaaaa tattaataat ttaacagaaa atgagaaact
    20221 cataagcagc aaaatattat atgacaatac aaactatttg gccatatcca agcttaatat
    20281 gaattttata ataggaggca gcgatataga caaaaaattc cgtacagttg atttaaagcc
    20341 ctttgccgga gcagcatatg taaatctgca attaaatccg caaattctta tttatgaaac
    20401 accggataat gatgtaaaga caatcaatat taacaatgaa acaacagccg gagacttaac
    20461 cacatccata atgaacatca tgtcattcat aaaagaagta agggagaaaa aggattgtga
    20521 tatttaaaag aataatttct acaatactaa ttatattatt ttttacatgc caattttcac
    20581 aaatcgaagc aatgcccggg atttcagccc aagcttacat agtgatagac gggcagtcag
    20641 gaagaattct aaattcccat aacgcggaaa taaaacttcc tattgccagc acaacgaaaa
    20701 taatgacagc aattcttgca attgaaacaa tcgaagacct ggaacaaaaa atagaagtgc
    20761 cgcccgattg tacgggcata gaaggttcaa gcatatattt aagacccggc cagacagtgt
    20821 ctgtaattga tttgttgtac ggaaccatgc ttcggtcggg gaacgatgca gcagcaactt
    20881 tatctaaatt tgccggcagg aatgatcctg aaggatttgt atcattgatg aataaaaagg
    20941 caaaagagct tggcgcttta aatacaaatt ttgtaaatcc cagcggcctc catgatgaca
    21001 atcattattc aacagcctac gacctggctc ttataagtgc ctatgctatg aaaaatgaca
    21061 cattcaagaa aattgcctca gctgaaaagt acaaggctgt atccatgaat aatatgtttt
    21121 acaataaaaa taaggtcgtt tatcaataca aacacggtac gggaatcaaa attggcttta
    21181 caaaagcagc gggcaggtgc ctggttgcgt cagcagaaaa agacggtacg gaaattatag
    21241 cagttctttt aaatgacaac aactggttta atgaaagtta tcaaatattt gactgggctt
    21301 ttgaaaatta caaaagctac agtgtagtcg agaaagggca gtttgtaaaa tacacacctg
    21361 aaagaaatcc tgtctttatt ggagacagct tcaaatgttc atttacagac gaagaaataa
    21421 agcaaatccg atttgagccc ttaatagaac ctgagctgat gtcaaatgat acagaaacta
    21481 aaacagccgg aacctacaac atatatctgg gaaaaacaaa aatacactca ggaaccctga
    21541 taacagaata attatctaaa cccgctccgg cgggtttatt aatttacaaa atgaaatgtt
    21601 tataatataa ttataaagaa taaagaatta tacaaggaga aatcatggct gaagaaagac
    21661 tgcaaaaata tatggcacgg tgcgggatta cttcaagaag aaaggctgaa gaattaattc
    21721 tgcaaggcag tgtaaaggtt aacggcatta tagaaaaaga acttggaagc aaaataaatc
    21781 ctgaaaagga tgaggtaacc gtttacgata aaataatata tgaaaaaaaa gaacatattt
    21841 acataaagct atacaagcca gaaggttaca ttacaacggt gaaggaccag tttaaccgca
    21901 aaactgttct tgatcttgta aatataaaag atagaattta tccggtaggg agacttgact
    21961 atgatacttc cggattattg cttcttactg atgacggaga ccttgccaac aaattaatgc
    22021 accccaaatt caggatttat aaaacatatg aagcagaggt ctcaggcacc atcagcgaag
    22081 agtctataaa tttgttaagg acgggtctta taattgacgg ttataaaact gcccctgcca
    22141 gggtaaaaat aatggagcaa aagggaaata atacgtcggt tcaaataagc atccatgagg
    22201 gtaaaaacag gcaggtcaga aaaatgtttg aagcagccgg gaaaaaagtc attaagctga
    22261 aaagaatttc gtttggagat attgtcttag gagggctaaa accgggtcag tggaaatatt
    22321 taaccgatga agaagtaagc tttttaaaga ggtagctatg gacaagaagt atggaaaaat
    22381 tgcagaaatg gttcactttc tcataaaggc atcctatgaa aaaagggaaa atttgaattt
    22441 tcttgattta acatgcggaa acgggcatga taccttgttt ttaagcaatt tagcaggaaa
    22501 aacaggttct gtgacagcct ttgatataca gaatgctgca atagagagaa caaacaaact
    22561 gcttgaagca aacgggcaat ttaataacta cactataatt aaagacagtc atgaattttt
    22621 tgaaaggtat ttaacaggca atatcgatgc cgcagtttac aacttgggtt atctacctga
    22681 ttacagcaaa gaaatattta cgaaaccgga aactacaatc tcctctgtag ttcaattgct
    22741 gccttattta aagaactggg ggaaaatata tataactgcc tatattactc atgataaggg
    22801 gtatgaaatt aatgcaattg ctgaattctt aaaaaccttg aacaaaaaag aatacaatgt
    22861 aattcatata aatgttatca ataaagaaaa ctcacctccc gaattattta ttatcgagaa
    22921 gaaggcatga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgcaattat
    23041 atggaggaat tgtatggcag atgaaaaggt tctttatcaa aatattcctt ggtgcaaagg
    23101 atgcggtgta tgcgtaggat tttgccccgc aaaagttctt acaataaaaa atggtaaatg
    23161 ttatatagct cacagagaca aatgtatatt ctgcggacag tgcgaactga gatgcccgga
    23221 taacgctatt tatatcggag gtaaaagatg aataaaaaaa gatataaatt agtacaggga
    23281 aatgaagcat gtgttgaagg tgcaatagca gccggcatga gattctatgc gggttatccc
    23341 ataacgccct caacagaaat cgctgagatt tcatcccaaa agctgccaaa gcttggaggg
    23401 aagttcatac agatggaaga tgagatttcg agcatagcag ctattatcgg tgcttcgctc
    23461 acaggattaa aatcaatgac cgcaaccagc gggccgggat tttcattgat gcaggaaaat
    23521 atcggatatg cagccataac agaaatacca tgtgtaatag taaacgttca aagaggagga
    23581 ccttctacgg gacttcccac atcacctgcc cagggcgatg taatgcaggc aaggtgggga
    23641 acacacgggg atcatcctgc tattgccttg acgcctaatt ctgttcagga aacattcgaa
    23701 tacactgtta aagcatttaa tttagcggaa aaatacagaa cacctgtaat ccttctcatg
    23761 gatgaaacag taggacatat gagagaaaaa atggcttata agaacatttc tgaaatagaa
    23821 ataattaaca ggaaaaaacc aacatgcaag cctgaagatt atgttgctta cagacctgat
    23881 gaggatatga ttcctccaat ggcatcattc ggcgaaggat acagatacca tgtaacaggt
    23941 ttatcccatg atgaaaccgg ctttcccaca aacagcaatg aagtatctga aaaactaatt
    24001 aaaagattag ttgataaagt agaaaagaac attgatgatt tttcatattt tgaagaatat
    24061 aaattagaag atgcagaaat agtcataatt gcatacggcg gagtatcaag atcagccaaa
    24121 agcgcagtgg acgaactcag aaaaagaaat ataaaagcgg gtttattcag accgataact
    24181 atttggccat tcctggaaaa acaaataaac aaagtttctc tacatgcaaa agaaatatat
    24241 gtggcagaga tgaatttagg acagtattct ttggaagtag aaagagctgc atgtaaaaat
    24301 tgcatcgtaa aaggcataaa cagaataaat ggcgaattaa taactcctga agaaattatt
    24361 gaagcaatag gaggcgaaag taatgaactg taaccaaact actaaagaat actaccgcgt
    24421 caacaggctg ccccatattt ggtgcccggg atgcggacac gggacattga ccaacacggt
    24481 cgtaagagct attgaaaagc tagggcttaa taaagatgaa gttgtaatag tttcgggcat
    24541 cggctgttca tcaagggctc ccggatacct ggattttaac accctccata caacacacgg
    24601 acgtgcattg gcttttgcaa caggcataaa actggcaaat ccgaaactgc atgttattgt
    24661 aataatgggg gacggagaca gttctgctat cgggggcaat cacctcattc atgccgcaag
    24721 aagaaatatt gatataacta caatagtttt caataataac atttatggta tgacaggagg
    24781 acaatcttcg cctactactc cttttggcga ttttgcaacc actgctgctt acggtaatat
    24841 agacaaacct tttgaccttt gcagcctggc acagggggca ggagctacat atgtaggcag
    24901 agctactgct tatcatgcgg ttcttatgca aaaattggta gaaaaaggaa tacgaaataa
    24961 aggattttct tttatagaag gcttaagtct ctgcccaaca tattacggta gaaataacag
    25021 aaagggcaat cccttaaaaa tgcatacatt tttcaaagaa cactgcattg ataaaaagtt
    25081 agttgataaa gacccgcaaa aaggtatttt caaaattttg attggagaat tctacaacaa
    25141 tcctcaacct gaatatattg aaagctatca agtaattatc gacatgttaa aagaataggg
    25201 ggaaaaaatg aaaaaacatg aaattagatt aagcggttca gggggtcagg gagttatatt
    25261 agcctccata atattggcag atgctgcaat tgaacaagga ttaaatgcaa tacagacaca
    25321 atcctatgga cctgaagcca gaggtggtgc cagcaaggct gaagtaataa taagcaatga
    25381 agaaattaat tatcccaaaa taaacaactc cggcatcctg ctttccctta ctcaaggtgc
    25441 atacgacaaa tatatcgaat acctggataa ggatggaata ctgatagttg acgattatgt
    25501 caaattatcg ggaaaggata cggctaaggt ttacaaactt cctatattga aaaattcaca
    25561 ggagaaattt gaaacatcaa tggtggctaa tataatttca ttaggtgcga tttatgaatt
    25621 aataggggaa gacacaattg ataagaatat tatgatacag tccattactg aaagggttcc
    25681 tcctatgaca actcaaaaga acatagaagc atttgaggaa ggaagaagat tagtaagagg
    25741 ttattatgaa cagtgattta atgagtaaaa taaaaaataa ctttcacaaa ttcagcaaag
    25801 ggcaaaagct gatagctcag tttattattg agcactatga taaggccgca tttatgactg
    25861 ctgcaaaaat agcggacagg gtagacgtaa gcgaatcaac cgttgttaga tttgcatcat
    25921 ccctggggta ttcaggatat ccggaaatgc agaaggcttt acaggtttta ataaaaaaca
    25981 agcttactgc agttcagaga ataggtcttg acgctgatgt taatgacaag ttaaagattc
    26041 ataaaaagat tttaaaaaat gaaatgaaca acatgcgatt tttgtttgag cattttgaca
    26101 tgggagcatt agacagggca acggaactga ttttaaatgc aaacagggtg tttgtactgg
    26161 ggcttaggac ttcttcaaca ctgtcaaact atctgggctt ttatctggac gtaattttgg
    26221 acaatgtcaa agtacttaac aacagcggag tcaattcctt gtatgaggaa atcataagag
    26281 ttaaagataa tgatttaatg attattataa gttaccccag atattcaaag acaacaattg
    26341 atgcagcaaa atttgtcaag gaaaagggtg taaaaatagt tgctataacc gataccgaag
    26401 aatctccggc ctactctctg gcagatgcat ccctgttggg aaagagcaat attgtatcat
    26461 ttgttgactc actggttgtt cccatgacaa taattaataa tttgataatt aacatcagct
    26521 tgaaagaaaa agaagaaata gttgaatatt ataatatatt ggaaaaactc tgggacaaca
    26581 acagcatgta tcagaacttt tagttgttct aatcttattt ttacagctta aatgcagaaa
    26641 aaacagtggt atggcattcg catgggttcc cgaatatgca gccagaggga gcaatctttt
    26701 ggcgaatgag actgcgtgaa ttaatatatg gatacacatc tttgtttagc ggcaggggga
    26761 cacaccttct tttgtgatag tctttgggaa agaaggaatg tccccctgtt taagtgtttg
    26821 ccgcaggtga aatgtcccca aaagtttttg ttgttatatt ttgttttatg taatataatg
    26881 ttaaagtgtt gaaataaaca tataaacatt gtccatggcc taagaaagga aaacaaaatg
    26941 ataatcgcaa tagacggacc gtcaggagcc ggaaaaagca ctgttgcaag actattaagc
    27001 aaagaacttg gatttgagta catcgataca ggggctatgt acagggctct tgcatataag
    27061 gcatataaaa aaaatctggt tataaacgaa gctgaaattg aagaaatgct taaaacaacc
    27121 aatatttcct actatgacaa caagatattc ttagatggtg aaaacgttga aaacaaaata
    27181 agggaggaga caatatccat tgctgcttca aaagtatccg ccctgaaaat tgtaagagaa
    27241 aagatggttg aaatccagag aaaaatcgct gcagataaaa atgctgtgtt ggaaggccgc
    27301 gatatcggca caatagtatt tccggatgcc gattacaaat ttttcataac cgcatccttg
    27361 gaagagaggg caaaaaggcg ttatgcacag ttgatagcag gcaatattaa tgctgaatat
    27421 gaaaatgttc tacgggatat gatagaccgt gatgaaaatg attcgaaaag gcagttttct
    27481 ccccttaaac cggcagaaga tgcaataata atcgacacaa ccaaaatgga cttatcagaa
    27541 gtgacaaaaa ctattgcagg atatatcgga ggaaataatg ttttataaaa tagcagtgtt
    27601 tgtatgcagg ataattgcat ttattatctt tgactttaaa gtttataata aagagaatct
    27661 tgacaataca aaaggcggtt taatagtctg tggaaaccac aggtgcatga ttgacccggt
    27721 gatgctggcc atatcaacaa ggcgccagat tcactacatg ggcaagaagg aattgtttga
    27781 aagcaaattc tggagctata ttttcagaag tcttggtgca tttcccgttg acaggaaggg
    27841 tgtctcctta tcttctataa aaagttctct taatatttta aacagcggcg gggtgcttgg
    27901 aatatatcct gaaggtacaa gagtaaagac agggtatgac gaaaaaaacg cgaaatcagg
    27961 aattgctttg atagcaaata aagcaaatgt taaaatatta cccgtatatt tggacggacc
    28021 atacaagttc agaggaaagc taaggcttta tataggaaaa gaaaaagact actttgaaaa
    28081 ctacaccggt aagcttaatt ctgacaaata tactgaaatt ggaattgaga ttttaaaaga
    28141 tatttataaa ttaaaagatg aaggtattaa atgaaaattt ttatttccga atataacgga
    28201 ttttgcggag gtgtcaaaca agctgtatta aaagcagaca gaattctaag tgaagaaagc
    28261 aaactttact gctatggaga aatcattcac aacaaagacg ttgttgataa atttaaagaa
    28321 aaaggaatgg taattgttga cgaaccgcct gatgacaacg atgcaaagct tttaataaga
    28381 agccacggtg tgggaaagga tatttatgat atcctggaag aaaaaaacat tgaaattgtt
    28441 gatgcaactt gtgtaaaagt aaaaaaaata cataaaatag ttgaagaatt caaaaataac
    28501 ggttacaata taatcattgt gggggacaaa gaccatcctg aagtccaggg aatagcaggc
    28561 tggtgcaata aagaagctga tattataaaa ggccccgggg aacttgaaaa aatcatcgac
    28621 ctgtcaaaga aatattgttt agtctgccag acaacattta atttgaatat ttttgaagaa
    28681 atattaaaaa ctattgaaat aaacaatttt tctaatataa ttgtacatga taccatatgt
    28741 gatgcaacaa ggaaaagaca ggaagcatgc atggctcttg cttcaaaatg tacggttatg
    28801 cttattgtag gcagtaaaaa cagttcaaat acaaaaaagc tgtatgagct gagcaaagaa
    28861 ttgtgtgaaa acacattctt gatacaaaac tataaggaaa taccttataa ctatattgat
    28921 aaaaatacta ttgtgggagt atccgcaggg gcttcggctc ctgactgggt aattgaggag
    28981 gttattgaaa tgttggaaaa tttaaatgac aacatgattg aaaagaacga acaggcagaa
    29041 attgcagaac agtctgaaga tttagaaaag ccggaacttt tgggagaagc aaaaacagct
    29101 gagcaggcag agccggctga agaagcagaa ccggtagaag cagctgagca ggcagagccg
    29161 gctgaagaag cagaaccggt agagtcagct gaacaggcag agccggctga agaagcagaa
    29221 cctgtagagg cagctgaaca ggcagagccg gtacaaccgg aagcagaaga aaagcaggag
    29281 caggtacaag agactaaaca aacagaaaaa aaagacagta tgcatgaatt gcttgaaaat
    29341 tatggcggag tagtctatat cagaactggt gaaaaggtta aaggtacagt aatatatgta
    29401 agccctgatg ctgtttctgt taacatcaat tataaatcag acggtatcat aacaagagat
    29461 gaattttcaa tgacagaagt tgctgattta agagagatgg tgaaggaagg ggatgaaatt
    29521 gaagcagtag ttgtaaagat ttcagaccag gacggaaatg ttgtactgac cagaaaaccc
    29581 ttggaagaac agaaaatctg ggacgatttg gaacagcaga gaatccgtgg agaaatcatt
    29641 gaaattccaa tagttgaagc atctcaatac ggaatttacg gaaaattgaa tggcattaag
    29701 ggttttattc caagaaacca aatatcaatt aaaagggatg ttgatccttc agaatatgta
    29761 ggaaagaatc tgaaggttaa aatattagaa acaaagaaca aaaaagggag aaagcagctg
    29821 gttttaactt ccagatctgt tgaaaagatt gaaaaggaat taaaggacaa ggaagtttgg
    29881 gaaaccatcc aggaaggaga agtctatgaa ggtacagtta aaaaccttca ggattacggt
    29941 gcatttgtag aaataaacgg aatagacgga cttcttcata tcaatgaaat agcatggacc
    30001 agaattaaac atccgtcgga agtattaaaa cccggtgaca aaatagatgt aaaagtaaaa
    30061 agtatagaca gggaaaacaa aagactttcc ttaagctata aggcaacggt aaagagccct
    30121 tgggcaaaat tcctggataa ataccaaaag ggtgatacag taacagggct ggtaactaga
    30181 attgttgact tcggagcctt cgtaaaggta gatgacattg agtgtttgct ccacattaaa
    30241 gatttagact gggcaagaac agaaaaggta acggatgttc ttcaggaagg acaggaagta
    30301 acagcaaaaa tactcagcat tacacgtaaa gacagaaaag taggcttggg attaaagcaa
    30361 ttgaccgagc atccatttga tgtttatgca aaaactctca acaaggacga cataatacag
    30421 gttgaagtaa cacgtatact ccttgacgga atacatgtta acgctaatgg caacattgac
    30481 tgcttcatac cgattgcaaa ggtatcaaat gaaaaattga ggacacctgc acaggttgtt
    30541 aaggttggag aaataaaaga cgctaaagta caaagtatag acatgaaagg caaaaacctt
    30601 aacctgacat tcatattaga tgacaagggt gaagaaatca ccgaaaagat aacttatcag
    30661 ccggaagatg caaacttcac cataggagag tccatagggg acgccctgga tgatttgtta
    30721 aagtaatttc taaaatgtta ggtcgaaaga tctaacattt ttnnnnnnnn nnnnnnnnnn
    30781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30841 nnnnnnnnnn nnnnnnnnnn nnggatgatt tgttaaagta atttctaaaa tgttaggtcg
    30901 aaagatctaa cattttttgt atgccctaac cccagatgtt cgagctatag cgaagaacat
    30961 atggcggtag gtcaaccaca acgagttccc aaaatatgcg accaaaggga gcaaatattt
    31021 tggtgaacgg agactgcgat taggagggac aatgcttaag gaatttttat ttgatgtatg
    31081 ctcaaaccat tttgtttcag gttttgaaca tattaacgga gacattttaa caaaatattt
    31141 taaaccatac acagatacat tggaaaagga caagcttgga agctatattt tcaaaagaac
    31201 agggacaagg gatacaaaga taatgcttgc cgcccatgtt gatgaaatag gccttatgat
    31261 tacaaaaata ctggagggag gttttttaag cttcactaca gtgggaggaa ttaatcctgc
    31321 ttccctggtt gcccaggatg ttacaatata cggaaaagaa aatatatttg gagtcatagg
    31381 gattaaacct cctcatatta caagtgagga agaaagaaca aaaccacata aaataaaaga
    31441 cctctttatt gacaccggat acacaaaaga aaaacttgaa agcttagtaa aaataggaga
    31501 tatagccact gtaaacagag agcccctatc attgcaggga aatgtcataa gcgccaaagc
    31561 atttgatgac agggcatgtg ttgcagtatt gcttgaaaca gcaaaagaat taagtaaaat
    31621 atcccataaa agcaatgttt attttactgc aacagcacag gaagaaaccg gcatgagagg
    31681 tgcaacaaca agcagctata aaatcgctcc tgacatagga atagttttgg atgtaggatt
    31741 tggcaaaact ccagatatgc ctcctgatgc acctgaattg ggaaaaggtc ccattgttgc
    31801 atatgggggg agaatgaatg caaagcttac aaagaaattt gttgaaacct gtaaaaaata
    31861 caactatcct ttgcagcatg aagcagttcc tgcaagaacc ggtacagaca cagaagcatt
    31921 gcatatatca agagacggaa ttccatgcat cctcttatca atacctcttc gctacatgca
    31981 tacatcagtg gaaaccattg atttatacga tgtggaaatt acaggaaaag caatagcaag
    32041 attcataaac gaactggata attctgattt ggaggaagta ttatgttttt aaaagaattg
    32101 actgagcttt ccggcgtatc aggttgcgaa tatgaagtaa ggaactttat aaaagataaa
    32161 ttatcccaaa tgggatgtga tttttatgtg gataagcttg gaaacataat agctcacaat
    32221 aaaggcagaa aaaataaaac cataatggtt gctgctcaca tggatgaggt aggactcata
    32281 gtttcaaaca tagattctga agggtttata aaatttaaag cagttggagg aattgaccaa
    32341 agagttttaa attcaaaggc agtcctggtt ggagaaaata aaattccggg agttatcggc
    32401 tcaaaagcag tacatttgat gaccagccag gaaaaagagt cctccctgcc aatagataaa
    32461 ttatacatcg atattggtac aaactctaaa gaagaaacag aaaaactagt caatatagga
    32521 gattatgtat catttaactc cagttatgta gaattcggag ataatctaat aaaagcaaaa
    32581 gctttggatg acagaacagg ctgcagtatt atactggagc ttatgaacca aaagttggat
    32641 gcggattttt acggtgtatt cactgttatg gaagaagtgg gtttaagagg agctgaaacc
    32701 gctgcatatc aattggagcc ggaccttggg atagtattgg agggaaccgt ttgtgcagac
    32761 atgcctgagg tagaggaccg ggataaatca accctcatag gcaaaggggc agcattatcc
    32821 ttgatggatg caaccacagt atatgatatt gatattgtaa gaagtgttgc aaaaattgca
    32881 gaaggcaata atattatgta tcaatacagg acttcagcat caggaggaaa tgacgcaggt
    32941 gctgtccata agacaaagag cggcgccaag gcagttgcaa tttcaactcc ctgcagatat
    33001 atacattcat cggttaacgt agcaagcaaa aaagacattg aaagtgtata tatgcttgca
    33061 aaacaagtga ttcttgaaaa ccagaaagga gagcaataat aatgtttaac agtgaattaa
    33121 tgaaaagatt atccgattgt ttcggtcctt cgggaagaga aaaaatgatt cgcgaaatga
    33181 ttaagaatga aataaaagac tatgcagatg aaataaccac agaccctctc ggcaatctta
    33241 tagcaagaaa aaagggcacc ggtaaaaaaa tattattttc agcccatatg gaccagattg
    33301 gtctgattat aacccatgtg gatgaaaaag gattccttag atttgccaat gtgggaggtt
    33361 tgaatgcaaa agaattattg ggtcttcgca tggttttcgg caatggactt gaaggggtta
    33421 tctgccagga agaattaaaa gacaaggaaa aactcacaac aggcaaatta ttcctagatg
    33481 ttgcttctac agataaagaa ttcattaaaa agaatttccg cacgggagat atgtgcgtat
    33541 ttaaatcaga gtattacgaa acaaaggatt gtgtcatctg caaggctgca gatgacagaa
    33601 taggctgcta tgtattgatt gaagcacttc aaaaccatcc tgaaacggat aatgacgtat
    33661 attacgtatt ttccgtacag gaagaagtag gctgcagagg agctaaaact gcaggctaca
    33721 gcatagaccc tgaactggcg attgctcttg atgtaacagc aaccggtgac agtcctgacg
    33781 gaataaaaat ggatgttaaa ttagggggag gggcagctat taaattaaag gacaattccc
    33841 tcataactca ttctgatgtg aaagacctct tgacacatct ggcagaaaaa aacaacataa
    33901 aataccagta tgaaatcctg gaatttggcg gtactgatgc aggacaaatc catatttcaa
    33961 gagaaggcgt acccagcggt gtaatatcca taccttcaag aaacctccac agctcaggag
    34021 aaatatttaa taaggctgat gtggaagaat gcattaaact ggtaattgca gcagcagaat
    34081 aaatgaactt aattggacag cgggacatat cgtcactgtc ctatttttat gataagagga
    34141 cggagcagcg tcatcataca atccctttct tgtgacaaag tgacgagggt agtgtcataa
    34201 cattgggttt actttgtgat aaatttatga taacattatc cttgaaaata tatctttcac
    34261 aatttggaag catcaaaaat tcttcagatt agtcgggaca gtcaatgtgt cccatttacc
    34321 aaaaaagcgg gacaatttag atacgtcccc actgtcccac agggagggta aaatggataa
    34381 atcaaaagaa atagatatgg aagccgtagg tctggaatgg gaaaagatga ttaacaatca
    34441 gcctgtggat gaaaatgttg tgtcttctat tatactaaag tcctggcacc gcagtgcttt
    34501 agcaggtgtt aatccttata ctgttgatga aaacctctat attaaagcag aagatctggt
    34561 acaatattac gattcaacca aaaaggttat agatgatgaa tttagatcaa tattacataa
    34621 aattgctgat gaatttgatt tatctttttt gctatatgat aataaatgta aaatagtaag
    34681 tgtactagca gtttcaaaaa attataaaac tattggatat agcggttcag aagatatagt
    34741 aggaacaaat tcaatctgcc tggctttaca gtatgataca cccatgcagg tattcggtaa
    34801 agaacactat tgtaaaatcc accataatta taactgttct gctgcaccga tacatgactt
    34861 agacaataat ataatcggag caattaatat cggcggaatc ataagccata aacatacatt
    34921 agaaacattg ggactagccc agtctatcgc taaaataata gaagataaaa ttcaaataaa
    34981 tcaaatggta gcacaactga ccatatctaa ccaaacactg aataatataa tcgaatacga
    35041 cagctcagga atcatttatt ttgttggcaa tgaaataatt agctataaca aatcacttct
    35101 taacatgttc gaaataatgt caactgataa tttgataata gtacatgaaa gtatacataa
    35161 tatactaaat cagttaaaag agcatcttca tcatgatgaa atagaaaaca tagaaatagt
    35221 tttaaatatt aataatgaaa agaaatcctt tattacatcc ataaaacaat tttcaattga
    35281 tgaaaacaaa gcagcttgcc gcctgtataa atttgacagc acaaacaagt atatgcgctt
    35341 aaatattaat aacagggcag tatatacatt tgatgatata atcggtgaat gcgagaacat
    35401 ggtaagacta aaagaattag cgaagaaaac ggcaagaact tcttcatcaa ttttaatatg
    35461 cggtgaaagc ggtacaggca aggaactgtt cgctcattca atccacaatg aaagtttcag
    35521 aaaaaacaag ccctttgtag caataaactg cggtgcaata ccctcagaat taatagaaag
    35581 cgaattattc ggttatgaac cgggagcatt tacaggagca tcaaacaaaa ttaaatttgg
    35641 caagttagaa attgcatccg ggggaacatt cttttttgat gaaatagaca gcatgccgtt
    35701 aaaggttcaa ataaaacttt taagagcatt gtcatccaac aggattacca ggattggggg
    35761 cataaaggaa atccccatag acataaggat aattgctgca acaaaaaaag acctgcttga
    35821 agaaatagaa cttggtcaat tcagggaaga tttatatttt cgtataaata ttataaattt
    35881 gacgatacct cccttaaggg acagaaaaga tgacattccc atacttgcgg aaagctttat
    35941 taattataat gaaaaacatt caaacatacc tgatgttaaa gttgatccag agttttatga
    36001 agctttgaaa tattattttt ggagaggaaa tgtaagggaa cttagaaatg taatagaaaa
    36061 agcagtcttg ctaaatgata aagaaacact tacaattaac aatcttccca gggaaattgt
    36121 aagcgcctat aagtataaaa gtcttaaaag taaattatct gcaaatatta ttgaaaccag
    36181 ggatgataag aatctgctta agacaggcga agaaataatc atagaatttg tgctaaatga
    36241 agaaaattgg aatttgacga atactgcaaa acgcttaggt ataagcagac caacattata
    36301 cagaaagatt gaagaatcgc cgaaattaag caaacgtaaa aaaatgttac agtaagaaat
    36361 atcgcaacaa tattttacag ttagaaaatc tgattaaaat gctgatatat ggaaaagaca
    36421 tgaaacaagc aaatattgta atataatttt acaaacagca gaatgctttt atggtagttt
    36481 gctgtttttt tctgctttaa tatttgtatt gttataatac agtgtttctt aaatatagaa
    36541 gaaaactcct atgttttcaa cgtttcatga actacctgac acattaatat tgaaaaaaaa
    36601 taagtattaa ttcatatatt tattgaaatt ggcacaacaa ttgcacgaat cataaataga
    36661 aacaatgaag gttttaaaac tgcattagta gatggataat aataaattat aacataataa
    36721 aaaaggagga aatttaatgt tatacggtta tgcaggaaag atgctgtttg taaacttatc
    36781 aaacgacgaa attaaagaag aaatacttac aaaagaatta gctgagaaat ttattggtgg
    36841 ttatggcttc ggtgcaaaaa tactttacga aatgatgccg gcaggggcag atcctttagg
    36901 acctgaaaac attttaggat ttcttacagg acctacaact ggtacgaaat cgtttttttc
    36961 aggaaggtac acgatagttc acaagtctcc ggttacagga ggctggaatg atgcaaactc
    37021 aggtggttac tttggacctg aacttaaaaa agcaggttat gatgccatat ttattaatgg
    37081 aatttctgaa aagccagttt atatttatat taatgatggt aaagtagaaa tccgagatgc
    37141 ctcacacctt tggggaatgg atacaaaaaa agtatggagc gtattaaaag aagaaacaaa
    37201 agagcaaaga ctacgtatct cagcaatagg acctgcagga gaaagattat cctatatatc
    37261 atgtccaatt aatgatggac accgtgctcc cggtagaggt ggtggtggag cagtaatggg
    37321 atctaaaaat ttaaaggcaa ttgctgttag gggaacaggg gaaattcctg ttaaaaatcc
    37381 ggaaaaactt ttagaaataa ataaaaaaat aagtgctgtt atgaaggaaa gtcagactgc
    37441 atatgctttc aggacatggg gcacaggagt tggaactcct gcatccgcat taagtggtga
    37501 ctcacccgtt aaaaattggg gaggagttgg agttttagat tttgaagacg ataaagctca
    37561 aaaagtggga gctatcgctc ttgatagata taaaacaaaa aaatatagtt gtgccaactg
    37621 ccctttagga tgtggagcgg aatataaggt ggaagatgga agatggccta ttgagcaaac
    37681 cgaaagacct gaatatgaaa ctgcagctgc ttttggctcc acatgtcttt gttcagaaga
    37741 agatgctctt cttatgtgta atgaaatatg caacatttac ggaattgaca ctatttctgc
    37801 cggtatgaca gtagcctggg ctatggaatg ttacaacgaa ggtgttttaa gcaaagaaga
    37861 actggatggc attgatttaa cctggggcaa tggagaagct atggtggcat tgttgcaaaa
    37921 aatggctgat ggagatggtt gtggaaagat attagccaac ggtagtcaat atgcagctaa
    37981 atactggggt aaaggccaag aatatttaca aacagcatca ggaattgaat tacctatgca
    38041 tgacccaaga tttgcacctg gattagccag aacatatcaa tttgacccaa caccgggacg
    38101 ccatgttaaa ggcggaatag gttctttaca aatgggtgga aatgagttaa cagacaagta
    38161 tgtttacaca gatacaggaa tgatggattt agtattaact acaaatactg aaattatgaa
    38221 ctctagtggc ttgtgctatt tccatgcctt agtatcacct cctggagtgc agaatgaatt
    38281 tattagtgca ataacaggga tggaatttga tgataaagaa tcattaaaaa ctggtcaacg
    38341 catctactta atgcgccatg catttaatgt aagagaagga ttaaagcctt cagactttaa
    38401 acttacaaga agagctgttg gtaaacctcc gttaacagca ggacctttgc aaggagtaac
    38461 tgtagatgaa gaactgttag gaaaaaattt ctttgaatct gcaggaattg actttgagac
    38521 cggcaaacca tcactggaat tattacaaaa acttggcggt ctagacgaag taattgctga
    38581 tatgtacgta aaagcaggag aaaccgtata ggcgatgaaa ataaaaatga aatattttgg
    38641 attgattaca aatgatttag gctctgaagc tgagctgccg gaagggtcaa gtgtttcaga
    38701 ccttctcaat catatagtaa aacaagatcc aaattataaa gaaatattaa caagggcaac
    38761 cattttggtc aataattcca aagtagatat agatactgtt ttaaaacaca atgatgaagt
    38821 gatggtttta gctgtacttg gtggaggcta acagtacagt ccccctttac caacgagaaa
    38881 tccaagattt aatgtgaaca attggaataa tatcacaaat tataggcaat aatataattt
    38941 gtgatatttt taatttaagg aatggggaca cacctctaaa gt
//
LOCUS       DAOPUT010000006        38599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_989_length_38599_cov_18.938097, whole genome shotgun sequence.
ACCESSION   DAOPUT010000006 DAOPUT010000000
VERSION     DAOPUT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 38599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38599
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_989_length_38599_cov_18.938097"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..599)
                     /gene="moaC"
                     /locus_tag="PKK75_01365"
     CDS             complement(126..599)
                     /gene="moaC"
                     /locus_tag="PKK75_01365"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNZ82274.1"
                     /translation="MDLTHINEQGRAKMVDVSSKEETLREAAAYGCVYMKSETLQRIK
                     EGTIKKGDVLSVAQVAGIMASKKTSDIIPMCHPIMISGCDISFNLNFDENKVEITSTV
                     KTTGQTGVEMEALTAVAAAGLTIYDMCKAIDREMIVSDVMLMKKSGGKSGTFERK"
     gene            complement(601..>1215)
                     /locus_tag="PKK75_01370"
     CDS             complement(601..>1215)
                     /locus_tag="PKK75_01370"
                     /inference="COORDINATES: protein motif:HMM:NF018201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNZ82275.1"
                     /translation="VNISIDTLNKEKFKEITRLGDIGKVFSAIEKCMSIGLVPVKLNI
                     VLMKGINDDEIGDFLKLTTDNPIQVRFIELMPIGEGTRYFEKCSMKVEEILERYPELI
                     PVEDDSKVASVYKLPGAKGSVGLISPVSCKFCSGCNRIRLTATGTIKPCLHSAEEINL
                     KPYLNDEQKLIEVIEDTVYNKPEEHHLETDGKSDTDRMMFQIGG"
     assembly_gap    1216..1315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1316..>2725)
                     /locus_tag="PKK75_01375"
     CDS             complement(<1316..>2725)
                     /locus_tag="PKK75_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNZ82276.1"
                     /translation="WQNIRPLGEDIVENQLIVPSKHKIRPVDIGALIAGGINKVEVYK
                     KPSVGIIPTGTEIVEPGTELKIGDIIDFNSRTFQASVSDWGGLPNRYPIVKDDYELIK
                     QAVLKAVEENDMVLINAGSSAGREDYTAAIVRELGDLIIHGVAIKPGKPVVMGLIKGK
                     PIIGIPGFPVSAFFVMDEIVKPLVYSCQGLETEAKKMVAATLTKRCMSSLKYLEFVRV
                     KLGYIGGKYVTTPLTRGAGTTMSLVNADGVLEIDQNIEGIEAGTEVQVRLLTSEDKIK
                     NTLVCIGSHDPIIDIAADIMHSRNKKFFLSSSNVGSTGGLMALKTGETHMAPTHLLDM
                     ETGEYNRTYLKKYVPHKKIALVKCVNRIQGFMVQKGNPKNITKFEDLERKDVKFVNRQ
                     RGSGTRLLLDYNLNKLNIDFKNIKGYYREEFTHLAVAAAVEAGDADAGLGVFSAANMM
                     GLDFIPVCNEEYDLAIPEEY"
     assembly_gap    2726..2825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2826..3255)
                     /locus_tag="PKK75_01380"
     CDS             complement(<2826..3255)
                     /locus_tag="PKK75_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNZ82277.1"
                     /translation="MQNVFLSNYELKEALQIYLKKIEPLNTKTETINTWDANGRVTAK
                     AIFALLSSPQYNSSAMDGIVCKSEKTFEASDRNHIVLEEKTDYIVVDTGDPIPKEFDC
                     VIMVEDLIRVDETHVKIIKSAVPWQNIRPLGEDIVENQLIV"
     gene            complement(3265..4491)
                     /locus_tag="PKK75_01385"
     CDS             complement(3265..4491)
                     /locus_tag="PKK75_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNZ82278.1"
                     /translation="MELFNVLTVKETLKVIDKNFKYEMDGEVVTLAEAEGRILKHAIA
                     AKDNVPDFKRSTVDGYAVYSKDTFGASESIQSIFDLQGEILMGQETKGEISIPGQCYY
                     VPTGGMLPKGADSVIMIEYTEKLDETTILASKSTSPGENVIEVGEDIKKGEIVIQKGT
                     KIRAYETGVLASLGFYNIEVYKKPVIGIISTGDEIVHPSENIKIGQIRDINTYMLNSL
                     ALDKGCLPVRYGLIRDEYELLKSTADKALSECDIVLISGGSSVGKKDNTVKVIKDLED
                     SELLVHGISVKPGKPTIIGKSKEKIIFGLPGHPLSCAVIFQVVVKYYIDKITAHKEIS
                     YPVTCRFKFNYHKAKGREEYLPVTVEEKADEFFASPVFGKSGLITSFSKAWGYIKIER
                     NEEGIKEDQRVKVYKF"
     gene            complement(4499..4987)
                     /locus_tag="PKK75_01390"
     CDS             complement(4499..4987)
                     /locus_tag="PKK75_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNZ82279.1"
                     /translation="MIKTGILTISDKGSKNERIDLTGPAIKESLDKNIYSAEYYKIIP
                     DEMDLICEELICMCDELKLDLILTNGGTGFAKRDVTPEATLKVIQKETPGIPEAMRQM
                     SLSITPKAMLSRAAAGIRNSTLIINLPGSPKGAVENLSFVLPALPHGIEILKGTGGEC
                     AR"
     gene            complement(5066..6502)
                     /locus_tag="PKK75_01395"
     CDS             complement(5066..6502)
                     /locus_tag="PKK75_01395"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNZ82280.1"
                     /translation="MIYKNSINNNTNEEVFFPGGAPAVFVTIPENITVHLGSPTEAAE
                     NITVPFIDYIKNVASSELYPTWPENALRANIYAIVSIALNRFNLEWYRSRGYQFDITN
                     SPQYDQAYVKNRGTYENTDRITDEIFNDYVIRDDDINPLFTTFCDGRVAQCNGMFQWG
                     TVELAEQGYSPIEILKYYYGEDISIVENAPVSGIGLTYPGEPLKPGDSSLVILRQQLA
                     LNRIRGNFPAIPVIIPVDGYYGDTTEKAVREFQRVFNLPVTGIIDKGTFYKIRQIYLG
                     VTKLSEVKASQSIYKEVLEYSRLALIQGDIRPSVDLLQYFLNVLSVYYKSIPGVAYTK
                     VFDTQTRAALIEFQKRMGLPVTGILDRETGDLLFRTAFGILKTLPPQEIILPIIRYPG
                     IEFRKGMGLEHPPVFLIQLALNYISLTIPLIPPISEEGIFGEETENAVIAFQNIFGLE
                     PTGVVNEATWNELVRVYQELRLEAQSGA"
     gene            complement(6512..7027)
                     /locus_tag="PKK75_01400"
     CDS             complement(6512..7027)
                     /locus_tag="PKK75_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82281.1"
                     /translation="MYYNYFNNRNGKSKHKIYRFTPYQSQDAPSSQPSLNQNEETGFL
                     SVGVFTALQALPVKGAEVIVYDILYDGTEFIHSRLITDDEGRVPDIELPIHHSHIEPS
                     GSKRYYFTTYNLRVTADNYYPFNVIGFRIFPGMKTNYTIDLLPIIPGETTYTPEQTVI
                     IPPSPIDGSNF"
     gene            complement(7216..7908)
                     /locus_tag="PKK75_01405"
     CDS             complement(7216..7908)
                     /locus_tag="PKK75_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ82282.1"
                     /translation="MVITVENLVKDYGSARVLDIETLQLNSGINVILGSNGSGKSTLI
                     SLISGLLKPSSGNILYNGKKFDASIRNNISVLVQKPYLFRKSSYENIISGLKYRKVNR
                     DEIETRVDKHIHYLDVEDLLNKKSHWLSGGERAKIALLRTVVLETEVTMLDEPTAAMD
                     IESTFKAEVLLRDMASNKRTVIVVTHDVFQARRIADYVLFMDKGKIIEMGLKNDVLDN
                     PRSTLLKQILNK"
     gene            complement(7908..8576)
                     /locus_tag="PKK75_01410"
     CDS             complement(7908..8576)
                     /locus_tag="PKK75_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82283.1"
                     /translation="MFEFIKEFFTNYSDIYPVIALSLRVSLTAALVASLFGLTLGVIT
                     ALHEFKLKKPLMRVTNTLMSLPPVLIGLLVYMLISRKGPLGSYGLLFSPSAMIIAQCA
                     LVFPIIFGLTVSSVSEAAKDIELTCITLGAGRRDTILTILSECRMQLLSVVTAGFGRA
                     ISEVGAVMMVGGNIRGKTRVMTTYIALETGKGNFEEAVFIGFVLLFISFIINITLYRI
                     QRRE"
     gene            complement(8587..9369)
                     /locus_tag="PKK75_01415"
     CDS             complement(8587..9369)
                     /locus_tag="PKK75_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ82284.1"
                     /translation="MQEEPEEPEQAEERSFILATTTSTEDSGLLDFLLPKFKEKTGIQ
                     VDVVAKGTGAALELAKNGDADGLLVHAKAQEEQFIADGFATERFDVMYNDFIIVGPES
                     DPAGLLTNAPNDPIKAFVLISENQAAFVSRGDESGTHTKEKSYWKEAEITPEGEWYVE
                     AGTGMGAVLQMASETEAYTLTDRATYLSMKDDLDLVIVTEKNDKMYNQYGVMMVNPEK
                     YPIKEAEVQEFIDWILSDEGQGLIAEYGVEEYGQSLFVPNAE"
     regulatory      complement(9516..9630)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(9664..10242)
                     /locus_tag="PKK75_01420"
     CDS             complement(9664..10242)
                     /locus_tag="PKK75_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HNZ82285.1"
                     /translation="MEYRNATLKDIYGVSLIQKKYHISTISEEDKPDGFVTTLFNEEQ
                     FKELIEKENGLAIACDGGKVIAYAMAASWEYWSAWPLFQHMINDLPNNIYLGQVLSTV
                     NSYQYGPICIDKQYRNTEVLPNLFEFSRLQMKDRYHILITFINQINGRSYRAHVDKLG
                     LEVIKPFDFNNNHYYELGYDMSRKTPGSANLL"
     gene            complement(10446..11090)
                     /gene="trmB"
                     /locus_tag="PKK75_01425"
     CDS             complement(10446..11090)
                     /gene="trmB"
                     /locus_tag="PKK75_01425"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNZ82286.1"
                     /translation="MRLRYVPGQYQYLKTCKKAITEPEKTGYKLNKAFDNKNPIYIEL
                     GCGKGKFLRENALNNPNINYFGFENNVKVAYRCAKAAFENDPPNYYIVYSNGDMLKEI
                     FEAGSIDRIYLNFSDPWPKPSHCKRRLTHKNFLDIYRIVLSEDGEIHMKTDNDELFEY
                     SIRQLKTDKWEFIAVTRDLHKSIYKDDNIMTEYEERFVNEGKRINKLIVRKKNG"
     gene            11416..11949
                     /locus_tag="PKK75_01430"
     CDS             11416..11949
                     /locus_tag="PKK75_01430"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNZ82287.1"
                     /translation="MDFLMNLQGPILYFTIFFAKIIEVSISTIRIVFVNKGERAKGAI
                     LGFVEIMIWLVVVSSVLNNITEDPIKVFIYAAAFALGNYFGVTIESKIAVGLASIQAV
                     VGQKDGEILADTLRDQGFGVTIIEGKGKNESVKNLLFIQLKRRKIPEAVRLIKEHNPE
                     AYITVNDIKSMLGGYIK"
     gene            complement(11985..13307)
                     /locus_tag="PKK75_01435"
     CDS             complement(11985..13307)
                     /locus_tag="PKK75_01435"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNZ82288.1"
                     /translation="MLKVAKFGGTSLADAAQFKKVYEIVKNNNERKYVVVSAPGKRFK
                     DDNKITDLLYLVYAHLKYSVPYDPVLKIIEERFNLIKKELNLNTDLDREFKTIRQSLD
                     SKCDEDYVVSRGEYLCGLLMADYLECDFVDARDVILFNYDGTVNQERTREKLTEALSK
                     SNKAVIPGFYGSYPDAAIKTFSRGGSDITGAIVSSIMGADIYENWTDVSGFLMADPRI
                     VKNPKQIKKITYEELRELSYMGASVLHEEAVFPVRQAGIPINVKNTNDPENPGTLIIR
                     DDQAGEDYANGHIITGIAGKKDFSFFYIHKEHMANEVGIIKKALEVFEERGISIDHIP
                     SGIDSFTIVLPSESVARISHQIVEELKEKCKTENVQVYKNISLITTVGIKMAYRPGIS
                     ARLFTALGNKNINIRMINQGSSEINIIVAVEDKDFEEAIRAIYDAFVK"
     assembly_gap    13595..13694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13695..14182)
                     /locus_tag="PKK75_01440"
     CDS             complement(<13695..14182)
                     /locus_tag="PKK75_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ82289.1"
                     /translation="MENSLKITGAPSLEELHRSPAYPPEDRLKKGPIAIIECIEEIPC
                     NPCEASCPSGAITIGDSITNLPRMDFEKCIACGMCIPACPGLAIYIKDYNYSDTKALL
                     SFPYEYYPLPKVNDIVQAVDRNGNILCTAKVLKVRNLKSNDHTAVITIEYPKEFFEEA
                     VN"
     gene            complement(14182..15279)
                     /locus_tag="PKK75_01445"
     CDS             complement(14182..15279)
                     /locus_tag="PKK75_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ82290.1"
                     /translation="MIQTELAIVGAGPAGLSAAIEAGRAGAEVLLIDENARPGGQLFK
                     QIHKFFGSRAHKAGTRGVNIGRSLLKESEELGIHTCLKGEVCGIEEGQNLWVVIDRKR
                     SEIVKARKIILATGATENATAFPGWDLPGVMGAGAAQTMINVNRVLPGRKVLMLGSGN
                     VGVIVAYQLMQAGAQVVAVLEAAPRLGGYGVHTAKVSRAGVPFHVSHTIKQAFGYDHV
                     EAVEIVELDSSFNQIPGTEKILDVDTVCLATGLTPLTELAWLAGCEFKFIPALGGHLP
                     IHDDRMHTTRDNIFIAGDISGIEEASTAMEEGRLAGINAACELGYISDEEAKLRTKEI
                     WDSLNSLRSGYFGLKRRNSKDLMLQTGKEVI"
     gene            complement(15276..15602)
                     /locus_tag="PKK75_01450"
     CDS             complement(15276..15602)
                     /locus_tag="PKK75_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNZ82291.1"
                     /translation="MNRIINHPILDEEKPAKEVLIFVDDKPIVAREGEMIAAALIANG
                     IYVNRYTMHKGKPRGIYCAIGRCTDCIMTVDGVPNIRTCVTEVKEGMRITTQKGAGVW
                     KEEGRQ"
     gene            complement(15612..16490)
                     /gene="dapA"
                     /locus_tag="PKK75_01455"
     CDS             complement(15612..16490)
                     /gene="dapA"
                     /locus_tag="PKK75_01455"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNZ82292.1"
                     /translation="MKNNWGRIITAMVTPMDAYGNINYDKALELAKLLIKEGSTALVI
                     NGTTGESPTVTTDEKLKLLSTIKSAVDVPVIAGVGTNSTAATIENAAAAEKTGVDGLL
                     VVVPYYNKPNQESLYEHFAQVAKAIKLPIMMYNVPGRTGVNMTAKTSVALSKIPNISA
                     LKEASGDLNQLATVVRDAEEDFLVYTGEDAQILPVLAVGGYGVVSVTAHIVCKDIKKM
                     IESYLSGNIIEAAKIHRKLIKITETMFMTANPIPVKAALNLLGMDVGITRLPLSAPSQ
                     EVIAALKDSMSELGLL"
     gene            complement(16480..16776)
                     /locus_tag="PKK75_01460"
     CDS             complement(16480..16776)
                     /locus_tag="PKK75_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNZ82293.1"
                     /translation="MCDELICRCEEVSREEIEKAIEEGAVTINEVKRFTRAGMGLCQG
                     CTCRRLVERILSEKTGVPLSEIQPSTYRQPVRPVQSDLIQEHNNNKSEGGFNEE"
     gene            complement(16868..18349)
                     /locus_tag="PKK75_01465"
     CDS             complement(16868..18349)
                     /locus_tag="PKK75_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNZ82294.1"
                     /translation="MSSQGIIIWIATFALCALYFFIAIKQKKEAELSFSNYAIGGGKI
                     PFFLLFFTHFANIMGVGNFMGHAGSAYINGLPWLAFIVGEQGSKIIFAIFFAGLAGRM
                     TYNTFPEMIDDLITRDKVTRALAGVLASSIMIAWVGGQGKAFGQLFATFTGVSPEPII
                     IIFTVMFVVYTTMGGMLSLIWMDFIQGLICVVFGAIFYIVAFSKIDFSMAVLGQQLAA
                     VGKAELFTFAGTNTMSLLTKFVTGCIGILVAQLYWQPCYAAKNPLVARRSMFFGGGVA
                     IVYTILTAMVGLIILTLNQGLDANSAMPWFMLNEVAPVVTVMIFILVFAAGMSSADSN
                     LNAAAILITNDLVRPFRKKEMTDAELIKLTRTLTIVIGAAAAFGGIYASTIMSLFSKA
                     YSMAGAGLVPLLVIGLLWKENSAAEHTMSKKNSRITPWGARTGIVAGSVLSQLPALGP
                     NAVLIALTVSAVCIVVISLLTKNVKNDPRFVSEGNVNPLIKEY"
     gene            complement(18423..19589)
                     /locus_tag="PKK75_01470"
     CDS             complement(18423..19589)
                     /locus_tag="PKK75_01470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ82295.1"
                     /translation="MNLEKDVCVIGAGVMGLATAYYLLKYGKTVAVLEKGEVGSGASS
                     SCDDMILMQSKKPGIALTLAMESLDMYKELPLELDADIGFLTLGGMVIIEDEKQLKIM
                     EDFVKQQVSYGLDVEIVDRDVLFKKQPHVAGYMIASTYSPSDSQVNPLLLMRALLRKG
                     LNMGMDIFRSTPVQEIKQSGDHWIVKGKNGISVECDSIVIAAGAWSGQIGDLLGIDIP
                     IRPIKGQIAITEQIAPIGETNCWTAAYIASKHDPSIMPDRSDYDKEIGLGFAFSRTSE
                     GNYLIGSTRENAGFDKTTNFKAIARILEQTRQYFPIMSQVNIIRTIAGFRPGTKDGSP
                     IIGGIDGFPGLFIAAGHEGDGIALSPITGKLTAEMVCGIEHKRLEELNLRRFKA"
     gene            complement(19607..20665)
                     /locus_tag="PKK75_01475"
     CDS             complement(19607..20665)
                     /locus_tag="PKK75_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNZ82296.1"
                     /translation="MEIVVVKFGGTSLATQMERKEAIAHIASLKTAGKSIIAVVSAMG
                     RKGSPYATDTLISLLGKGCNPAVLDLMMSCGEVISACVLTDSLVQQGIDAVALNSVQA
                     GIFTNGIFGSADIFDIDTKRIKMELLEGKVVVVTGFQGMSVRNDITTLGRGGSDTSAV
                     VIGGFLRAGSVHIFTDVPGIAVIDPRIVPEAKYMEYVDIKHMYALACWGAGVVHPRAI
                     FEAQKYNLELFVRSTFDRGQGTVIMKDMEMRGPVGIGIIRGCSLYDAEQSDSLIISSV
                     KKSVRLTSDSGYSLLTAVFSGYSRDEMSEAIGRLPVKSELYFNQSCAHILVETENVES
                     SAYSLYNRLFGKGGHPLI"
     gene            complement(20829..21812)
                     /locus_tag="PKK75_01480"
     CDS             complement(20829..21812)
                     /locus_tag="PKK75_01480"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HNZ82297.1"
                     /translation="MKIRIGIAGYGNIGRGVETAVRDSSDTELAAVFTRRNPDELKIQ
                     SPGVPVVNFSLAEEYKDIIDVIVLCTGSAKDLPLHGVQFAKLFNTVDSYDNHAKIPAY
                     LKSINESALSSGKVSIVSIGWDPGLFSIIRMISEAVLPNGSSYTFWGKGVSQGHSDAI
                     RKVDSVKYGIQYTIPSESAVDRVRRGETPNLTVRDKHMRECFVVCSEDGDKNKIEADI
                     VSMPDYFADYNTKVNFISEEEFKKEHSKMPHGGLVIRNGKTGGGKNNHTIEFSLKLDS
                     NPEFTGCILLSYARAAYRMNMEGMKGAKTVFDVPIFYLSSRNREDLIKELL"
     gene            22105..24435
                     /locus_tag="PKK75_01485"
     CDS             22105..24435
                     /locus_tag="PKK75_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNZ82298.1"
                     /translation="MKNMLCSICKKNVAVIYVNKIIEGKMQTTGLCIPCAKKQGIAPM
                     DQIMRQSGMKPEDFENINNQLTEIIGDMDLEKLSQDMPPENSDGINSLMNFMNNAFTG
                     STSSTNSEFLPSEDEQNADYAGTGSGTGPKIKEKVKRKKLKYLDTYGTNLTEQARFGE
                     LDRIIGREREIDRVVQILNRRSKNNPVLIGEPGVGKTAIAEGLAVRIAEKQVPVKLFN
                     TEIYLLDLTAVVAGTQFRGQFEARMKAIIDEAKKAGNIVIVIDELHNIVGAGEAEGGA
                     MNAANILKPALAKGEVQIIGATTLDEYRKHVEKDTALERRFQPVMVDEPSVEDTIEII
                     KGIKDYYEHYHNVKITDEIVKQAVIMSERYITDRFLPDKAIDVIDEAGSRANLKNKGL
                     VELEALRSELSRIREEKEEAAENDDYERAADLKVTEIRIEAKLNDLSKEYENILLTEE
                     DIAYVIEAWTKIPVQKISEDEADKLLHLEERLHRRVVGQHPAVTSLAKAIRRNRSGFK
                     KKKKPSSFIFVGPTGVGKTELARTLAYELFENEEAMIRLDMSEYMEKHTVSKLIGAPP
                     GYVGFDQAGFLTEKVRRKPYSVILLDEIEKAHEDVFNILLQILEDGRLTDNQGRTVNF
                     ENTVVIMTSNAGTSIKANRIGFGNTDYEAMESKIKDALRQTFRPEFLNRIDDIIVFSE
                     LSREELSEIVDLMLKEVKKDAEEKKITISVSDKMKDHILEKGYNTKFGARPLRRAIQK
                     YVEDEISEAYLRGVLKENSSVNVDVNDEGKTVLTII"
     gene            complement(24483..25325)
                     /locus_tag="PKK75_01490"
     CDS             complement(24483..25325)
                     /locus_tag="PKK75_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ82299.1"
                     /translation="MSIRIVIDSTSDVTDEIIEKYNLKVVPLTVNFEKQSYLDKIELS
                     SKEFFEKLAAADKLPTTTLVSPGAFVETFSEILLEGDKVLGLFVASEFSGTYDSARMA
                     RNMIGSDDIHIIDTRSVCLGSFALILEAIELVKENKTIEEIVEILNRLKEKIVVVAAL
                     DTLKYLEKGGRLSKGQAVVGSLLNIKPIVAIKDGEIKVIEKLRGKNKTVKWFDEWIEK
                     NNYDLSDKTVLLFHGNAYNQLKLLRETIEDKYKIKNIIENEIGPVIGTHSGPGVLGMG
                     FLNK"
     gene            complement(25424..25876)
                     /locus_tag="PKK75_01495"
     CDS             complement(25424..25876)
                     /locus_tag="PKK75_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNZ82300.1"
                     /translation="MSNHETVNEILVRLFANILDIEEKCLKIGEFSDLSITEMHVIDN
                     IGMTNERTMSDTAKAVKVTSGTLTTAIDNLINKGYVERRRSSEDRRVVKIRLTEKGRE
                     AFKSHEEFHKDLVISGLQQLDSHEEEVLIKVLSNIDVFFSEKYKYAGW"
     gene            complement(25931..26527)
                     /locus_tag="PKK75_01500"
     CDS             complement(25931..26527)
                     /locus_tag="PKK75_01500"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNZ82301.1"
                     /translation="MKLNRNFYNRDARIVGEDLIGKILVRKYDDIVIKSRIVETEAYI
                     AAIDKASHGYGGKITPRTKIMFGPPGYAYVYFIYGMYYCLNFVTEEEGTCSAVLIRAA
                     EPLINEEEMSINRYNKNLNELTNYQKRNISNGPGKLCNALKITTDHNGIDLTGDEIYV
                     EDDGFKGFETVISKRINIDYAEEAKDFLWRYTIHYNNA"
     gene            complement(26546..27373)
                     /locus_tag="PKK75_01505"
     CDS             complement(26546..27373)
                     /locus_tag="PKK75_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNZ82302.1"
                     /translation="MPKLTEIPKRRIRKVAGKFIDSHAHLDDDRFEGDRDEIINSLYD
                     NDIEAVLNPGADLKSSKDAVLIAEKYPFIYAAVGCHPHDSKYMSDESMNIFKEMAKNN
                     KVIAIGEIGLDYYYDNSDRVTQRKWFREQIRLAKELDLPYIVHDRDAHEDILNIMKEE
                     YYDGVRGIMHCYSGSVELSREFLKLGFYISLGGPVTYKNARVPKLVAKEVPFDRLLIE
                     TDSPYLTPAPFRGKRNEPKYVKYVAEEIAGIRNLSVDDLSEKTRENFKRLFNLRQGT"
     gene            complement(27321..27782)
                     /locus_tag="PKK75_01510"
     CDS             complement(27321..27782)
                     /locus_tag="PKK75_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82303.1"
                     /translation="MKEGMEVLLLLNEDDKTVHLLSRLLMAVMILITGIMIRGLMETK
                     TYTGEDIMVLAVLPVLVIALYVPLSRKTLISTLGIHKRSNLVRWENIKGINYLKPDVK
                     NKVKVKIIYMMMNKETSINLTFLNDEEQLEKFREYAKTYRNTKKKDKKSGR"
     gene            complement(28010..28549)
                     /locus_tag="PKK75_01515"
     CDS             complement(28010..28549)
                     /locus_tag="PKK75_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ82304.1"
                     /translation="MELGDKIRSLRQKQKISIENLSEMSGLSKGLISQIERDITGPSV
                     ASLWKISKALNVTMNYFFDEYNGLNQAVLKDERKKIIIGKGSRTYELLSPDLKKQIEM
                     LMIEINPNEKPETELISHEGEKCGVVIKGTLRVISGSKVYDLNEGDSIYLDSTIPHKY
                     INTGEEISVSVWALVPPSF"
     gene            complement(28617..29435)
                     /locus_tag="PKK75_01520"
     CDS             complement(28617..29435)
                     /locus_tag="PKK75_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82305.1"
                     /translation="MWKKKNKQVAFISKEHDEYIKSINKEKRFIRNTQVLIFVFGIIL
                     WETAARNNLIDTFLTSSPGAIFELIVKFVGDGSLFKHLYISTMETIGGFFAGTLGGLL
                     IAILLWWFRKLARILDPYIVILNSLPKTALAPIIILWVGMGYSGIIATAASISIVVTI
                     MTLYTGFAGVETDKITLLKTMGANKFQILKKVIIPANIPNILSVIKVNIGLSWIGVIV
                     GEFLVSKAGIGYLILYGSQTFKLDLVMMGVFVLGVIAAIMYFFSSWMEKTIIKY"
     gene            complement(29416..30174)
                     /locus_tag="PKK75_01525"
     CDS             complement(29416..30174)
                     /locus_tag="PKK75_01525"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82306.1"
                     /translation="MEYICELKNISKRFHTINNETKAIDDISFKVKKGEIVSIVGPSG
                     SGKSTVLSLIAGLEKPSEGEVIVNTKSIGFMFQKDQLYEWRTVYKNCLLGLEVRKMDN
                     EENKIKVKKMLEKYGLTNFKNHYPSQLSGGMRQRAALVRTLAIEPDLLLLDEPFSALD
                     YQTRLEIGDEVGTILRNEKISTILVTHDIPEAVSISDRVIVFTERPANIKKEYNIEFE
                     ECVGERTPMKCRNTLVFGEYFRNIWRDLDVEKEK"
     assembly_gap    30600..30699
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            30787..31005
                     /locus_tag="PKK75_01530"
     CDS             30787..31005
                     /locus_tag="PKK75_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNZ82307.1"
                     /translation="MKLVDVGVGKTVKVEQNSARDYLKERFLALGLTKGADVEVLRKG
                     PKNNLTVYRIRGAMIALRNEESRLIEVL"
     gene            31018..32940
                     /gene="feoB"
                     /locus_tag="PKK75_01535"
     CDS             31018..32940
                     /gene="feoB"
                     /locus_tag="PKK75_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNZ82308.1"
                     /translation="MGLTAQSVRLESLKDIYEIEKKDKQYIIALAGNPNTGKSTVFNY
                     LTGLNQHTGNWPGKTVVSARGQFKYNENEYIMVDLPGSYSLFTASKDEEVTRDFLCHG
                     NYDAVVVVADATCLERNLNLAFQVSELTNRIILCINLIDEARKKNIIIDKEGLENELG
                     IPLVLTSARSGQGMDELKNLIEKTAHKSTVDGAEELLYFNQDEEIREKISEDNYNYAN
                     RLKQKYVKEDINKLNRDKKIDDIITSKKFGIPIMLALLGLIFWITITGANMPSQILAD
                     ILFVFQYKLDAAFISLGANDFIRGILVDGMYRSLAWVVSVMLPPMAIFFPLFTLFEDL
                     GYLPRVAFNLDHMFKKAGAHGKMCLTMCMGFGCNAAGVIGCRIIDSPRERLIAIITNN
                     LVPCNGRFPLIIVLSAAFFAFTGTFLDSIIPALFITLMVLMGVFATLTVSFLLSKTIL
                     KGIPSTFTLELPPYRAPQAGRIIYTSLLDRTIFVLSRAVIVAAPAGAATWLLANIYVD
                     DMSILIHAANYLEPLGRAIGLDGFILIAFIAGFPANEIVIPVLLMAYMSTGSLTDFES
                     ISLLRQILLSNGWTALTAINVMLFSLLHWPCSTTLWTIKKETCSLKWTFAAFIIPTAL
                     AFLACFATATVYRLIL"
     gene            complement(32922..33104)
                     /locus_tag="PKK75_01540"
     CDS             complement(32922..33104)
                     /locus_tag="PKK75_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82309.1"
                     /translation="MPGIKESKMQQNDLRKVYLEITEHCILNCTTCFRQNWTDAPAAM
                     SYDLAFMGENHYKINL"
     gene            complement(33105..33326)
                     /locus_tag="PKK75_01545"
     CDS             complement(33105..33326)
                     /locus_tag="PKK75_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNZ82310.1"
                     /translation="MIEIRLFATLRDGREKIIKLDPNSFTIAGDVLKHLSICPEDVAI
                     YLINGRHSNLDAPIKDGDVIAIFPPVGGG"
     gene            complement(33327..35156)
                     /locus_tag="PKK75_01550"
     CDS             complement(33327..35156)
                     /locus_tag="PKK75_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNZ82311.1"
                     /translation="MTTAYNGKILRVNLTTGEVKPEELNMDVAKKFIGGRGLGTKFLL
                     DEGVATVDPLSEDNKLVFINGPMTGTSVPTGGRYMVVTKSPLSGMIASSNSGGVWGAK
                     LKYAGWDAIIVEGKAKEPVYINIDDEKAEILPANEYWGKTTEDVVEEMLKLYPKASIL
                     NIGPAGEKLSLIAAVMNDKDRAAGRSGVGAVMGSKNLKAISVTASRTVIEPFDTDMLR
                     QAASDSQKKIRENGVTSGGLPTYGTSVLVNIVNNLGSFPTQNWQKSYFETSDKISGET
                     LKENHLVKQGFCHRCPIGCGRVIDFKGKIIGGPEYEPIWAYGANCGVDDLPAINEANY
                     MCNEYGLDAISAPCTIAAAMELYQRGYITDEDCDGAPLNWGSGKAIVEWTKRMGQGYN
                     ELAKLMSKGSYRLCEHYGVPEYSMSVKKQEMPAYDARGIQGIGITYATSNRGGCHVRG
                     YMISPEVLGLPEALDRFTTEGKAKWAKTFQDLTACIDSMGMCLFTSFALGAPEYAALL
                     NAATGTDYTAERLLEVGERIYNIERLFNKLAGMKPEDDRLPKRLTEEPIPEGPSKGEL
                     SKISVTLPDYYNVRGWVDAFPTNETLMRLGLEEEMLKEEALVE"
     gene            complement(35400..37040)
                     /gene="gltX"
                     /locus_tag="PKK75_01555"
     CDS             complement(35400..37040)
                     /gene="gltX"
                     /locus_tag="PKK75_01555"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNZ82312.1"
                     /translation="MDFKKLAELLYPNITKTIEYYENEAYPKRDLPEGAKVTRLAPSP
                     TGFIHLGNLYGAFVDERLAHQSGGVFLLRIEDTDEKRKVEGSVETIISSLEYFGLKFD
                     EGAGIEGERGAYGPYFQSSRAEIYQCYAKHLIETGRAYPCFCSEEELEDLRKAQIGEN
                     VNTGYYGKWARDRNLSLEEVREHLDNNEEFVVRFRSEGTEEGKFEIDDAIRGHLSIHE
                     NFQDIVLLKTNGIPTYHFAHVVDDHLMRITHVVRGEEWLSTLPIHYELFKALGWETPV
                     YCHTAHLMKIDDGTKRKLSKRKDPELGLGYYMELGYHPKAVREYLITILNSNFEEWRM
                     ENPDRPIDDFQFTLEKMSNSGALFDLDKLNNISKDVLLKIPAEEIYEFLHSWLNEYKK
                     EAFELLSSNKDQVIKLLSVGREGNNPRKDLMYCEQIFDFISYYFDEYFKIVDSYPENI
                     GVEEAKKLLQAYLNTYDHSDDQSQWFEKIRVIASENGYAAKPKDYKKNPDIYKGHVGD
                     VSTVIRIALMGRASSPDLWELQQIMGEEKVRRRIKKSF"
     gene            complement(37172..37243)
                     /locus_tag="PKK75_01560"
     tRNA            complement(37172..37243)
                     /locus_tag="PKK75_01560"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37208..37210),aa:Glu,seq:ctc)
     gene            complement(37347..37772)
                     /locus_tag="PKK75_01565"
     CDS             complement(37347..37772)
                     /locus_tag="PKK75_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82313.1"
                     /translation="MISTRWFQGIDNLSVVLDIRKEVLLEADKLTDFYDEFAFNAVLY
                     EDDLPAGTGRLLFKEGKYFIDKLCLKEQFKGLNYEDLLVRLLVRKAVTIGAGKTYMEA
                     DENLEKTLENIGFIKASEEETLMVKEGDVGGCCGNSSEK"
     gene            37962..38159
                     /locus_tag="PKK75_01570"
     CDS             37962..38159
                     /locus_tag="PKK75_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ82314.1"
                     /translation="MLGERLRQIRKENNLTKQELSLALNVPKERIKLYETGDEIPSDF
                     YLSSFVQYFNISKEELIELIK"
     gene            38308..38556
                     /locus_tag="PKK75_01575"
     CDS             38308..38556
                     /locus_tag="PKK75_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HNZ82315.1"
                     /translation="MDKRIGVIGIVIEDMSQAAEVNNILHEYSHIIIGRMGIPYRERG
                     LNVISLIVDGTNDDIGALSGKLGKIKGLSVKSALSKKH"
BASE COUNT        10944 a         7926 c         6501 g        12828 t
ORIGIN      
        1 cccttttttt tcgcttatat ttacagctaa tacttttgcc attttattct ccatttcttt
       61 atcattttct ttcaaataat actgacctgc ataaaagttt tagtggcttt ttggtttcta
      121 tagttttatt ttctctcaaa tgttcctgac ttgccgccgg attttttcat cagcattaca
      181 tctgaaacaa tcatttctct gtctattgcc ttgcacatat cgtatattgt cagtcctgca
      241 gctgcaaccg ctgtcagtgc ttccatttca acccctgttt gacctgtggt tttaactgtt
      301 gatgttattt caaccttgtt ttcatcaaaa ttaagattaa aacttatgtc acagccggaa
      361 atcataattg gatggcacat ggggattata tcagaggttt ttttggaagc cataattcct
      421 gctacctgtg ccacagaaag aacatctcct tttttgatag tcccttcctt tattctttgg
      481 agggtttcgc ttttcatgta aacacagccg tatgccgcag cttctctcaa tgtttcttcc
      541 ttgctgctta catcaaccat tttagctctg ccctgttcat taatgtgagt taagtccata
      601 ttaacctcct atttggaaca tcatcctgtc cgtatcgctt ttgccgtctg tttccaaatg
      661 atgttcttca ggtttattat aaacagtatc ttcaataact tcaatcagct tttgttcatc
      721 atttaaatat ggctttaggt ttatttcttc agctgaatgg aggcatggtt ttattgtgcc
      781 tgttgcagta agtctgattc tgttgcagcc gctgcagaac ttgcagctta caggacttat
      841 aagtcccact gacccctttg caccgggcag cttgtaaaca gaagcaacct tgctgtcatc
      901 ctctacggga atcagttcag gatatctttc caatatttct tcaaccttca tgctgcattt
      961 ttcaaaatac ctggttcctt cgccgatggg cataagctct ataaacctta cctgtatggg
     1021 gttgtccgtg gtcagcttta agaaatctcc tatttcgtca tcatttattc ctttcatgag
     1081 cacaatattc aatttaaccg gaactaagcc gatactcata catttttcta ttgcagaaaa
     1141 aaccttgcct atatctccaa ggcgtgttat ttctttgaat ttttctttgt tcaacgtatc
     1201 aatgcttata tttacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtact
     1321 cttcaggtat tgccagatca tattcttcat tgcatacagg tatgaagtca agccccatca
     1381 tatttgctgc agaaaaaact ccaagtcccg catctgcatc cccggcttca acagcagccg
     1441 caacagccaa atgagtgaac tcctccctgt aatagccctt tatattttta aagtcaatat
     1501 ttaatttatt taaattataa tcaagcaaga gccttgttcc ggaccctctt tgtctgttga
     1561 caaatttaac gtcctttctt tcaaggtctt caaattttgt aatattttta gggtttcctt
     1621 tttgaaccat aaatccttga attctgttta cacatttaac caatgcaatt tttttatggg
     1681 gaacatattt tttaagatag gtcctattgt attctcctgt ctccatatcc agcaaatgtg
     1741 tgggagccat gtgagtctcc cctgttttta gtgccatcaa gcctccggtg cttccaacat
     1801 ttgaggatga caggaagaat tttttgtttc ttgaatgcat tatatcagca gctatgtcaa
     1861 ttatggggtc atggcttcct atgcagacaa gggtgttttt gattttatct tcagaagtta
     1921 aaagtcttac ctggacttca gtccctgctt caattccttc gatattttgg tctatttcca
     1981 aaacaccgtc cgcattaacc agtgacatag tagttcctgc tcctctggta agcggggtag
     2041 taacgtattt tccgcctata tatccaagct tgactcttac aaattcaaga tatttaagtg
     2101 aagacatgca tcttttggtc aaagtggcag caaccatttt ctttgcttcc gtttcaaggc
     2161 cttggcagct gtagacaaga ggtttcacaa tttcatccat aacaaaaaag gcagatacgg
     2221 gaaatcctgg aattccaatt atgggtttcc ctttaattag acccataaca accggtttgc
     2281 cgggctttat agccacacca tgaataataa ggtccccgag ctctctgaca attgctgctg
     2341 tataatcttc acggcctgcc gaggagcctg catttattaa aaccatatca ttttcttcaa
     2401 ctgcttttaa aactgcttgt ttaataagtt cataatcatc tttcacaatc gggtatctgt
     2461 ttggcagccc gccccagtca ctgactgagg cctgaaatgt tcttgaatta aaatctatta
     2521 tatctccgat tttaagttct gtccccggtt ctactatttc agttcccgta ggaatgatgc
     2581 ccaccgaagg tttcttgtaa acttcaactt tatttatgcc gcctgctatt aatgcaccga
     2641 tatcaacagg tcttatttta tgctttgacg gcactatgag ctggttttca actatatcct
     2701 ctcccaaggg tcttatattc tgccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnngcact atgagctggt tttcaactat atcctctccc aagggtctta tattctgcca
     2881 tggtactgcg ctttttataa tttttacatg ggtttcgtct accctgatta aatcttcaac
     2941 cattatgaca cagtcaaatt cctttggaat aggatctcct gtgtctacta caatatagtc
     3001 tgttttttct tcaaggacta tatgatttct gtcagaagct tcaaatgttt tttcactttt
     3061 gcatacaata ccgtccatgg cagatgaatt gtattgagga gaagaaagaa gggcaaaaat
     3121 tgccttggca gttaccctgc cgtttgcatc ccaggtattt atggtttctg ttttggtatt
     3181 taagggctct atctttttta aatatatttg taatgcttcc ttcaactcat aattggacaa
     3241 aaaaacattc tgcataaatt cctcctaaaa tttatatact tttactcttt ggtcttcctt
     3301 aatgccttct tcatttcttt caatttttat atagccccag gctttggaaa aacttgtgat
     3361 taagcctgat tttccaaata cgggggaagc gaaaaactca tcggcctttt cttccacggt
     3421 tacaggaaga tattcttccc ttcccttggc tttatgatag ttaaatttaa atctgcatgt
     3481 tacgggatat gaaatttcct tatgcgctgt aattttatcg atataatact taacaactac
     3541 ctgaaatatt actgcacatg aaagaggatg tccgggaaga ccgaatatta ttttctcctt
     3601 tgattttccg attatggtag gcttgccggg cttgacggat atcccgtgaa ccaaaagctc
     3661 gctgtcttca aggtctttga taaccttgac cgtattgtcc tttttaccta cggagcttcc
     3721 tccggatata agtactatgt cacactctga caatgctttg tctgcagtgc ttttaagcag
     3781 ttcatattcg tcccttataa gtccgtatct tacaggcagg catcctttat caagggcaag
     3841 ggaatttaac atataggtat tgatgtccct gatctgccct atctttatgt tctccgaggg
     3901 atgtacaatt tcatctcctg ttgatattat tcctatcacc ggttttttat atacttcaat
     3961 attataaaaa ccaaggcttg caagcacacc tgtttcataa gcccttattt tagtgccttt
     4021 ttgtattact atttcacctt ttttaatatc ttcccccact tcaattacat tttcgcccgg
     4081 tgaagttgac tttgaagcaa gaatggtagt ttcgtccagt ttttccgtat attcaatcat
     4141 tattacagag tcagccccct taggaagcat gcctcccgtt ggaacatagt aacattgtcc
     4201 ggggatggat atttctcctt ttgtttcttg tcccattaat atttcaccct gcaggtcaaa
     4261 aatcgactga atggattcgc ttgctccaaa tgtatccttt gaataaacag catatccgtc
     4321 aactgttgac cttttaaaat cagggacatt atccttggca gctattgcat gttttaaaat
     4381 tcttccctct gcttcagcta atgttacaac ttccccgtcc atctcatatt taaaattttt
     4441 atctattacc tttaaagttt cttttaccgt cagtacatta aatagttcca ttgctctcct
     4501 atcttgcaca ttctccccct gtgcccttta atatttcaat gccgtgtgga agtgcaggaa
     4561 gaacgaagct taaattttct actgctccct tggggcttcc gggaagattg attattagtg
     4621 tgctgtttct tatgcctgca gctgctcttg aaagcattgc cttgggtgtt attgaaagag
     4681 acatctgcct cattgcttca ggtatgccgg gagtctcctt ttgtattacc ttcagggtag
     4741 cctcaggagt tacatctctt ttggcaaaac ctgttcctcc gtttgtgaga attaaatcaa
     4801 gtttaagttc atcgcacata cagattaatt cttcgcatat caaatccatt tcatcaggta
     4861 ttattttata atattctgca ctgtatatgt ttttatccaa agattctttt attgccggtc
     4921 ctgtcaagtc aattctttca ttttttgaac ctttatcgct tattgtaagt attcctgttt
     4981 ttatcataga gtctccttat atttaaaaaa gtataacgat aaaagtatta taacaagtta
     5041 tacttttttg ttcaatagtc acttgttatg cgccgctttg agcctccaat cttagttctt
     5101 gataaactct gactaattca ttccatgtag cctcattaac aactcccgtc ggttcaagac
     5161 caaatatatt ttgaaaggca attaccgcat tttcagtttc ttcaccaaaa ataccttcct
     5221 cacttattgg aggtattaat ggtattgtta aagaaatata gttcagcgcc agttgaatta
     5281 aaaaaaccgg cggatgttcc aagcccatgc cttttctaaa ttcaataccg gggtacctta
     5341 taattggaag aattatttct tgtgggggca aagtttttaa aataccaaaa gctgttctaa
     5401 ataatagatc tcccgtttct ctgtcaagaa ttccggttac cggcaatccc attcttttct
     5461 ggaattctat aagcgctgct cttgtttgcg tgtcaaatac ttttgtataa gcaacaccgg
     5521 gaatgctttt atagtatact gataaaacat ttaaaaaata ttgcaataaa tctaccgacg
     5581 ggcgaatatc tccctgaatc aatgctaatc tcgaatattc aagaacttcc ttatatattg
     5641 actgacttgc ttttacttca gataattttg ttactcctaa atagatttgc ctgattttat
     5701 aaaatgttcc tttatctata ataccggtta ccggcagatt aaatactctt tgaaattccc
     5761 ttacggcttt ctccgttgtg tcgccataat aaccatctac gggtataatc accggtatag
     5821 ccgggaaatt gccccttatt ctgtttaatg ccaattgttg tctcaatatg actaaactgg
     5881 aatcaccagg cttgaggggc tccccgggat atgttaatcc tattccggaa accggagcat
     5941 tttcaactat tgatatatct tcaccataat aatattttaa tatttctata ggtgaataac
     6001 cttgttctgc taattccact gttccccact gaaacatccc gttgcattga gcaacccttc
     6061 catcacaaaa tgtagtaaaa agaggattaa tatcatcatc tcttattaca tagtcgttga
     6121 atatttcatc tgtaattctg tctgtgtttt cataagtgcc cctgtttttt acatatgcct
     6181 gatcatattg gggtgaatta gtaatatcaa actgataacc ccttgatcta taccattcta
     6241 gattaaacct attcaatgca attgacacaa tagcgtaaat attggctcta agtgcatttt
     6301 ccggccatgt agggtaaagt tctgaagaag caacattttt aatataatca ataaatggta
     6361 ctgtaatatt ttctgctgct tctgttggag accccaaatg gacagttata ttttcgggaa
     6421 tagtaacaaa cacagcagga gctccccccg gaaaaaagac ctcttcattt gtattattat
     6481 taattgagtt tttatagatc atggcagcca cctaaaaatt cgaaccatct atgggactgg
     6541 gaggtataat aacagtttgt tcaggagtat atgttgtttc acccggtata atcggaagca
     6601 aatcgattgt ataattagtt ttcattccgg gaaaaatacg aaagcctatt acattaaaag
     6661 ggtaatagtt atctgctgtt actcgtaaat tataagttgt aaaataatat cttttactac
     6721 cggatggttc aatgtgggaa tggtgtatag gcagttctat gtcaggtacc ctgccctcat
     6781 cgtctgttat cagacgtgag tggataaatt ctgttccatc atacaaaata tcataaacta
     6841 taacttcggc gccttttaca ggaagcgcct gcaaggctgt aaatactcct accgataaaa
     6901 atccggtttc ctcattttga tttaatgaag gttgtgaaga tggtgcatct tgcgattgat
     6961 acggggtgaa ccggtatatt ttatgtttgg atttaccgtt tctattgtta aagtaattat
     7021 aatacatata aaaccactcc ttgaaattat ctatcttaat gctatttgta ttatatgtaa
     7081 taatcggtaa attgatatta tgtattgttt ttattatgat cttagacata tgcttgcatg
     7141 atggtctttc aaggtacaga tttcttattt attgattgct tttgtcacct tatattataa
     7201 atttataagt tatacttatt tattcaatat ttgttttaaa agtgtgcttc tgggattgtc
     7261 cagaacatcg tttttcagcc ccatctcaat aatcttgccc ttatccataa agagcacata
     7321 gtccgcaatt cttctggctt gaaatacatc atgggttaca acaatcactg tcctcttgtt
     7381 tgaagccata tccctcaaaa gcacttccgc tttaaatgta ctttcaatat ccatggctgc
     7441 tgtaggttca tccagcatag ttacttctgt ttccaatacg actgttctta atagtgctat
     7501 tttcgccctt tcccctcctg aaagccaatg ggattttttg tttaataaat cttccacatc
     7561 taagtaatga atgtgtttat ctactcttgt ttctatttca tccctgttta cttttctata
     7621 ttttaatccg ctgattatat tttcatatga agattttctg aacaaatacg gtttttgaac
     7681 cagtacagaa atgttatttc ttatagaagc atcgaatttc ttgccgttgt ataaaatatt
     7741 cccgctgctt ggctttaata aaccagatat caagcttatc aaagtacttt ttccgcttcc
     7801 gtttgaacct aatattacat taatacctga gtttaactga agagtttcaa tatccaggac
     7861 acgggcgctg ccataatctt tgactaagtt ttcaactgta attaccatta ttccctcctc
     7921 tgtatcctgt atagggttat attgattata aatgatataa aaagaagtac aaatccaata
     7981 aaaacagctt cttcgaaatt accctttcct gtttctaagg caatataggt tgtcataacc
     8041 ctggtttttc ctcttatgtt tccaccaacc atcataacag caccaacctc tgaaattgct
     8101 cttccaaaac ctgctgttac aacagacaaa agctgcattc tgcattcgct taaaatcgtt
     8161 aatattgtat ctcttctgcc ggcacccaac gtaatgcatg taagctcaat atcctttgcc
     8221 gcttcagaaa cagaagatac agtgaggccg aatattatgg ggaaaaccag ggcacattgt
     8281 gctataatca ttgcagaggg cgaaaaaaga agcccatagc tacccaatgg accttttctt
     8341 gatattaaca tgtacaccaa aagccctatt aggacaggag gcagactcat gagggtgttt
     8401 gtaaccctca tgagcggttt ttttaatttg aattcatgta aagccgttat tacacccaat
     8461 gtcaagccga ataaagatgc aactaaagca gcagtcaatg atactctcaa tgaaagtgct
     8521 ataaccggat aaatgtcgct gtaatttgtg aaaaattctt taataaattc aaacatattt
     8581 caccttttat tcagcatttg gtacgaacag ggattgtcca tactcttcaa caccgtattc
     8641 tgctattaga ccttgtcctt catcagacaa tatccaatct ataaattcct gaacttctgc
     8701 ttccttaatt ggatactttt cgggattcac catcattaca ccgtattggt tgtacatttt
     8761 atcgttcttc tcagtcacta taaccaggtc aagatcatct ttcatagata aatatgtagc
     8821 tctgtctgtt aatgtatagg cttctgtttc agatgccatt tgcagtacag cacccattcc
     8881 tgttcctgct tcaacatacc attccccttc aggagtaatc tctgcttcct tccaataaga
     8941 tttttctttt gtgtgtgtac cggattcatc tcctctggaa acgaaagctg cttggttttc
     9001 cgatatgagc acaaaagcct ttatcggatc atttggagca tttgtcaaca aacctgccgg
     9061 atctgattca ggtcctacta taataaagtc attgtacatt acatcaaatc tttctgttgc
     9121 aaaaccatca gcaataaact gctcttcctg agcttttgca tgtaccaata aaccgtctgc
     9181 atctccgttt tttgccaatt caagggctgc gcccgtaccc tttgctacta cgtccacttg
     9241 gattcctgtt ttttctttaa atttcggcag taaaaagtct aataaacctg aatcctctgt
     9301 gcttgtagtt gttgcaagta taaagcttct ttcctcagcc tgctccggtt cttccggttc
     9361 ttcctgcatc tgttcttctt ccggctcctc tgcaggcggt tgttctgtct gctggcatcc
     9421 tgctgtgaac agtaatgtta acatcagtaa tgcaatcagc agctttctca tttttgtttt
     9481 cataaaaaaa ctcctttcaa attttgtata ataagaaaag agatgtccaa tctcctttcc
     9541 aatgggaggc agagccgttt ccagctatac cctgacgacc taaaattcat agtaaccctt
     9601 agaattttaa gttcggagaa aacgtactat tattttatat gaaaaacgca aaaaagtcaa
     9661 tttttataat aaattagccg accctggtgt tttcctggac atatcatagc ccagctcgta
     9721 ataatgattg ttgttgaaat caaatggttt tattacctca agtccaagtt tgtccacgtg
     9781 ggcacggtag gacctgccgt taatctggtt tatgaacgtt attaaaatat ggtacctgtc
     9841 cttcatctgc agtcttgaaa attcgaaaag gtttggcagt acttcagtgt ttctatactg
     9901 tttgtcaatg catatgggac cgtactgata ggagtttaca gttgacagca cttgaccaag
     9961 atatatgttg tttggaaggt cgtttatcat atgctggaaa agtggccatg ctgaccaata
    10021 ctcccatgat gccgccattg catatgctat aaccttgccg ccgtcacaag ctatggcaag
    10081 accgttttct ttttctatca gctccttaaa ctgttcttca ttaaatagtg ttgttacaaa
    10141 tccatcaggc ttatcttctt cgcttattgt tgaaatatga tacttttttt gaatcagtga
    10201 aacaccatag atatccttta gagttgcatt tctatattcc ataattttac ctctcaatat
    10261 attttttaat catacaataa ttctaccatg aatcgtatac aattaacagc atatttctgt
    10321 tttttcaggt aataattatt atgtgaagca tttaccaaac caggaggaat aaaattgaaa
    10381 aaatatagaa taaagtaaag gtaataaaat taattattga taagtagaac ataatatatt
    10441 ttatgttaac catttttctt tctcactatc aatttattta tccttttgcc ttcatttaca
    10501 aatcgctctt cgtactcggt cataatattg tcatccttgt aaatgctctt gtgtaaatcc
    10561 cttgtaaccg ctatgaattc ccacttgtct gttttcagct gccggatgga gtattcaaat
    10621 aattcgtcat tgtctgtttt catgtgaatt tcaccgtcct cggaaagaac aatcctgtat
    10681 atatcaagga agttcttatg tgtcagccgt cttttgcagt gagagggttt aggccatggg
    10741 tcggaaaagt taagataaat tctgtcaatt gaacctgctt caaatatttc ttttaacatg
    10801 tctccgtttg aataaacaat gtagtaattt ggagggtcat tttcaaaggc tgcttttgca
    10861 catctgtagg caactttaac attgttctca aatccaaaat agttgatatt aggattatta
    10921 agagcatttt ctctcaggaa ctttcccttg ccgcagccta gttctatata tatgggattt
    10981 ttgttatcaa atgccttatt taatttatat cctgtttttt ccggttctgt gattgctttt
    11041 ttgcatgttt tcaaatattg gtactgaccc ggaacatatc ttaatctcat tattttcttc
    11101 ctttgctttt aggtaaattt tattatacat tgtttaatag aaaaataaca gtatatttac
    11161 ataattttaa ttatgtaagc tggtatgttg aaatttgcgt taaagaagaa tatatttact
    11221 gaaataaaca tgttgaggtt atggcagatt aactataaaa gtaaaaaatg tatttacata
    11281 atcttaatta tgtaagccgt tattattgaa aattgtcgtt agaaaaaaat agctgtttaa
    11341 aataaattaa ttgagtctat tgcaaattaa acataatatt agtagaatga gtatatcaaa
    11401 ataaagaggt gacatatgga ttttttaatg aatttgcaag gaccaatact gtactttaca
    11461 atttttttcg caaaaattat agaagtttcc atttcaacaa tcagaatagt ttttgttaat
    11521 aaaggtgaaa gagctaaagg tgcaatattg ggttttgtag aaataatgat ttggcttgta
    11581 gttgtaagct cagttttaaa taatattacc gaagatccca taaaggtatt tatatacgct
    11641 gcagcttttg ctctgggtaa ctatttcggt gttacaatag aatcaaagat tgctgtaggc
    11701 ttagcttcaa ttcaggctgt ggttggtcag aaggacggag aaattcttgc tgatacttta
    11761 agagatcagg gatttggggt tacaataatt gaaggaaaag gtaagaatga aagtgtaaaa
    11821 aatcttttat tcattcagct taaaagaagg aaaatccctg aagctgtcag actaattaag
    11881 gagcataatc ctgaagcata cataactgta aatgacataa aatcaatgct tggaggatat
    11941 attaaataga tagacatggt atggggacac aagtgtcccc aaatttattt tacaaatgca
    12001 tcatatattg cacgaatggc ttcttcaaaa tctttatctt ccactgccac aattatgttt
    12061 atttcgcttg aaccctggtt aatcatgcgt atattgatat ttttgtttcc taatgcagta
    12121 aacagtcttg ctgaaatccc cggcctataa gccattttga ttccaacagt ggttattaaa
    12181 ctgatgttct tataaacttg tacattttct gttttgcact tttcctttaa ttcttccaca
    12241 atttgatggc tgattcttgc aacggattct gaaggaagta cgattgtgaa gctgtcgatg
    12301 cctgagggaa tatggtcaat gctaattccc ctctcttcaa atacttccag agcttttttt
    12361 attataccca cctcatttgc catgtgctct ttgtgaatat agaaaaatga aaaatccttt
    12421 tttccggcta ttcccgtaat tatatgaccg tttgcataat cttctcctgc ctgatcatcc
    12481 ctgattatta aagttccggg attttcagga tcatttgtat ttttaacatt gatgggaata
    12541 cctgcctgtc tcacgggaaa aacagcttct tcatggagaa ccgatgctcc catatatgac
    12601 aattccctta attcctcata agttattttc tttatctgct tagggttttt cactatacgg
    12661 gggtcagcca ttaaaaatcc ggatacgtca gtccagtttt catatatatc agcccccata
    12721 atacttgaaa caattgctcc agtaatatct gagcctcccc gtgaaaatgt cttaatggca
    12781 gcgtccgggt atgagccgta aaagccgggt ataaccgcct tgttgctttt acttaatgct
    12841 tctgtaagtt tttccctggt tctttcctgg tttactgtac cgtcataatt aaataatatc
    12901 acatccctgg catcaacaaa gtcacattcc aggtaatcgg ccatcagcaa tccacataaa
    12961 tattctcccc tgctcacgac ataatcttca tcacatttac tgtccaggct ttgcctaata
    13021 gttttaaact ccctgtctaa atctgtgttt aaattcagct ctttcttgat aaggttaaac
    13081 ctttcttcta taatctttaa aaccggatcg tagggtactg aatattttaa atgtgcatag
    13141 acaagatata ataaatccgt aatcttgttg tcatccttaa accttttgcc tggtgctgaa
    13201 accaccacat attttctttc attattattt ttgactattt cgtacacctt tttaaactgc
    13261 gcggcatcag ctaatgaagt gcccccaaat tttgcaacct tcaacatttc atcctccaga
    13321 ataatactaa ctttctaaca atatcacaag aggaatttaa tgtcaacaaa tttaatacat
    13381 atgtgtacat aatacgcacg gagcactaaa ttatcaggtg gattttacta gctacagttt
    13441 tcatgccaaa aaaaatatgc ccaaaagatt ccttgataat caagaaaaga ttaaggctat
    13501 tttacttttg aaacagcttt gccatcttat aatctcttca tattaacagc ttcttcgaaa
    13561 aactccttag ggtattcaat ggtaatgaca gctgnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnatatta acagcttctt cgaaaaactc cttagggtat tcaatggtaa
    13741 tgacagctgt gtgatcgttt gatttgagat tacgaacctt aagcacttta gctgtacata
    13801 gaatgtttcc gtttctatcc actgcctgaa caatatcatt gacttttggc aggggataat
    13861 attcataggg gaaagacagt agtgcttttg tgtcgctgta attgtaatcc tttatataaa
    13921 ttgctaaacc gggacatgct ggtatgcaca taccgcaagc aatacatttt tcgaagtcca
    13981 tacgaggcag atttgttatc gaatcaccta tcgttattgc accgctggga caagatgctt
    14041 cacatggatt gcaggggatt tcttcaatac attcaattat tgcgataggt ccttttttaa
    14101 gcctgtcttc cggaggatat gcaggagacc tgtggagttc ttccaaggaa ggagctcctg
    14161 taatttttaa cgaattttcc actaaatcac ctcttttccg gtttgaagca ttaaatcctt
    14221 ggaattccgt cgtttcagtc cgaaataccc gcttcttaag ctgttaagac tgtcccatat
    14281 ttctttggta cgcaattttg cttcttcatc actgatataa cctaattcac aagctgcatt
    14341 tatacctgca agccttcctt cctccatagc tgtgctggct tcttctatac cggatatatc
    14401 accggcaata aaaatattat ccctggttgt atgcatcctg tcatcatgaa ttggcagatg
    14461 cccgcctaaa gcaggtataa atttaaactc acagcctgca agccatgcaa gctctgtaag
    14521 aggtgtcaaa ccggttgcta agcacactgt atcaacatcc aaaatctttt ctgttccggg
    14581 gatttgattg aatgaactat ctaattccac tatttcaaca gcttccacgt ggtcgtatcc
    14641 aaaggcttgt tttatggtat gagatacatg gaaaggaacc cctgcccgtg ataccttggc
    14701 agtatggact ccatagcctc caagccttgg cgctgcttcc aaaacagcta caacctgcgc
    14761 accggcttgc ataagctgat aagctactat tactcctaca ttgcctgagc ccagcattaa
    14821 aacctttctg ccgggcaaaa cacgattaac attaatcatt gtttgtgcag cccctgcccc
    14881 cattacaccg ggaaggtccc agccgggaaa ggctgttgca ttctctgttg ctccggtagc
    14941 aagtattatt tttcttgctt ttacaatttc gctgcgtttt ctgtctatta caacccaaag
    15001 gttttgccct tcctctatac cgcagacttc gcccttcaaa caggtatgta tacctaattc
    15061 ttcactttcc ttcaaaagcg acctaccgat attcacacct cgtgttcctg ctttatgagc
    15121 tcgagaaccg aagaatttgt gtatctgttt aaaaagctga ccgccgggtc gtgcattttc
    15181 atctataagg agcacttctg ctcctgctct gcccgcttct atggctgcgg aaagccctgc
    15241 aggtcctgca ccgactatag ctaattctgt ttgaatcatt gccgaccctc ctctttccat
    15301 actcctgcac ccttctgggt ggtaattctc ataccctctt tgacttcagt tacacaagta
    15361 cgtatatttg gtacaccatc cactgtcatt atacagtctg tacaacggcc gatagcacaa
    15421 tatatccctc ttggtttacc cttatgcatt gtatatcgat ttacatatat gccgtttgca
    15481 attaaagcag cagcaatcat ttcaccttct cgtgcaacaa taggcttatc atccacaaat
    15541 attagtactt cctttgcagg tttttcttca tctaaaatcg gatgattgat tattctattc
    15601 attgctgcct cctagagaag ccccagctca gacatggaat ccttgagtgc tgcaattact
    15661 tcttgtgaag gtgcggataa tgggagacgg gtaatgccta catccatgcc gagaagattt
    15721 agtgctgctt ttacaggaat cggatttgct gtcataaaca ttgtttctgt tatcttgatg
    15781 agttttctgt ggatttttgc tgcttctatt atattgccgc tcaaatagct ttctatcatc
    15841 ttcttgatat ccttgcatac tatatgtgct gttacagaaa caacaccgta tcctcctaca
    15901 gccaatacgg gcagtatttg agcatcttct ccggtataaa ccaagaaatc ttcttcagca
    15961 tcacgtacta ctgttgccag ctggttcaaa tctcccgatg cttccttcaa tgcagatata
    16021 ttaggaattt tagaaagagc aacggaggtt ttggctgtca tattcacacc ggttcgtccc
    16081 ggcacattgt acatcattat gggcagctta atagcttttg ccacttgagc aaaatgctca
    16141 taaagtgatt cttgattagg tttgttatag taaggtacca caactaagag tccatcaact
    16201 cctgtcttct ctgctgcagc tgcattttca attgtagctg cagtggagtt agttccgact
    16261 cctgcaatta caggtacatc aacagcgctt tttatagttg acaggagctt taatttttca
    16321 tcggttgtaa ctgtgggaga ttctccggtg gttccgttta taacaagtgc cgtacttcct
    16381 tcttttatta aaagttttgc caattccaag gccttgtcat aatttatatt tccatatgca
    16441 tccatggggg taaccatggc tgtaattatt cttccccaat tattcttcat taaatcctcc
    16501 ttcagattta ttgttattat gttcctgtat caagtcagac tgtaccggac gtaccggttg
    16561 acggtaagtt gacggttgta tttcacttag aggaactcca gttttttcag acaatatacg
    16621 ctctacaagc ctcctgcatg tgcacccttg acataaaccc atgccggctc tggtaaagcg
    16681 ctttacttca tttattgtta cagcaccttc ttcgatagct ttttctattt cctctcggga
    16741 aacctcctca cagcggcata ttaattcgtc acacatagtt acctcaattt ctatagtatc
    16801 agagatattg tacataactt taattaattc attatgtaca atatctgcca aattttacaa
    16861 agatatatta atattcttta attaatgggt ttacatttcc ttcacttaca aacctggggt
    16921 cattttttac gttcttagtt aagagcgata ttacaacaat acacactgcg gaaaccgtca
    16981 gggcaatcaa tacggcattt ggccccaagg caggcagttg agaaagtaca gaccctgcta
    17041 ctatgcctgt acgtgctccc caaggtgtta tcctgctgtt tttcttgctc atggtatgtt
    17101 ctgctgcgct gttctctttc cataagaggc ctataaccag caatggtact aagccggcac
    17161 ctgccataga atatgccttg gagaacaagc tcattatggt acttgcatat atacctccaa
    17221 aagctgctgc agcaccaata acaattgtta atgttcttgt aagtttaatc aattcagcat
    17281 cagtcatttc ttttttcctg aagggtctta ccaggtcatt ggtaataagt atggctgccg
    17341 cattaagatt tgaatctgct gaagacatgc ctgctgcaaa caccagtatg aatatcatta
    17401 cagtaacaac tggagcaact tcattgagca taaaccaggg catcgctgag tttgcatcca
    17461 agccttgatt taatgtgaga ataatcaatc ccaccattgc tgttaaaatt gtatatacta
    17521 tagctacacc accgccgaag aacatggagc gtcttgcaac caaggggttc ttagcagcat
    17581 aacatggctg ccaatataat tgtgcaacga gaatacctat acatcctgta acgaattttg
    17641 tcagcagact cattgtgttt gttcctgcaa aagtaaataa ttctgccttt ccaactgctg
    17701 ccaattgctg acctaatact gccatgctga aatcgatttt tgagaatgca acaatataga
    17761 atattgcacc gaaaacaacg cagatgagac cctggataaa gtccatccaa ataagcgaaa
    17821 gcattcctcc catagtcgta taaactacaa acattaccgt aaatattata ataatgggtt
    17881 ccgggctgac acctgtgaat gtggcaaaca gctggccgaa tgcttttccc tgaccgccta
    17941 cccatgcaat cataattgat gaagccaata cacctgccag tgcccttgtt accttgtcac
    18001 gggtgataag gtcatctatc atctcaggga aggtattgta tgtcattctt cccgccaagc
    18061 ctgcaaagaa tattgcaaat attatttttg aaccctgttc gcctactata aaggccagcc
    18121 agggcaatcc attaatatat gcacttcctg catgacccat aaaatttcct acacccataa
    18181 tatttgcaaa atgcgtaaag aacagcagga aaaaaggtat tttccctcca ccgattgcat
    18241 aatttgaaaa actgagctct gcttcttttt tttgtttaat agctatgaaa aagtacaatg
    18301 cacacaacgc gaaagttgct atccaaataa taattccttg tgaactcata tttctactcc
    18361 tttcctttat gataagagcg atgataacgc tcttcttaaa tattattatt gttacttgtc
    18421 ctttatgcct tgaaccttct taaattaagc tcttctaatc ttttgtgttc aatgccgcat
    18481 accatttctg ccgttagttt tccggtaatc ggcgataggg caattccatc gccctcatgt
    18541 cctgctgcta taaacaagcc gggaaatcca tcgattccgc cgattattgg gcttccatcc
    18601 ttagtgccgg gtctgaaacc tgcaatggtg cggattatat ttacctggct cattattgga
    18661 aaatactgcc tggtttgttc caatattctt gcaatagcct tgaaatttgt ggttttgtca
    18721 aatcctgcgt tctctctggt gcttcctata aggtaattgc cttcactggt tcgtgaaaaa
    18781 gcaaatccca gtccgatttc tttgtcataa tcacttctgt cgggcattat ggaaggatca
    18841 tgcttagatg caatataagc agctgtccaa cagtttgttt ctcctatggg ggcaatctgt
    18901 tctgtaattg ctatctgtcc ctttataggt cttataggaa tatcaatccc caaaaggtca
    18961 ccgatttgtc ccgaccaggc acctgctgct atcactatgc tgtcacattc cacgctgatt
    19021 ccgttttttc cttttactat ccaatgatca cctgattgtt ttatttcttg aaccggtgta
    19081 ctcctgaata tatccattcc catattaagc cccttgcgta aaagtgctct cataagcaat
    19141 agcggattca cctgagaatc agatgggctg tatgtggaag ctatcatata cccggcaaca
    19201 tgaggctgct ttttaaaaag aacatctctg tctacgattt caacatcaag tccgtaagaa
    19261 acctgctgct tgacaaaatc ctccataatt ttaagctgct tctcatcttc aataatgacc
    19321 atgcccccta aagtcaaaaa tccaatatcc gcatcaagct caaggggcag ttctttatac
    19381 atatccaagc tctccatagc cagggtcaat gcaatacccg gcttctttga ctgcatcaat
    19441 atcatatcgt cgcaggaact tgaagcaccg ctgcccactt cccctttttc taaaacagca
    19501 acagtcttgc cgtattttag aaggtagtat gcagtggcaa gccccataac acctgcaccg
    19561 attacacaaa catccttttc taaattcatt attcctccat gctaagctaa attaaggggt
    19621 gacccccttt gccgaacaac ctgttgtata aagaataagc tgatgattca acattttctg
    19681 tttcaacaag tatatgtgca catgattgat tgaagtacaa ttctgatttg accggtaatc
    19741 tgcctatggc ttcactcatt tcatccctgc tgtatccgct gaaaactgcc gtcagcagag
    19801 aatacccgct gtcacttgtt aggcggacag acttttttac gctgcttatt atcagactgt
    19861 ctgactgttc tgcatcatat aagctgcagc ctctgatgat tccaataccc acaggacctc
    19921 tcatctccat atctttcata attactgtac cctgccccct atcaaatgtt gaacggacaa
    19981 aaagctcaag attgtatttt tgagcttcaa aaatagcccg gggatggaca actcctgccc
    20041 cccagcaggc aagcgcatac atatgcttaa tatctacata ttccatatat tttgcctcag
    20101 gaactattct tggatctata acggctattc ccggaacatc tgtaaatata tgtacagacc
    20161 ctgcccttaa aaatccgcct attaccacag cagacgtatc gcttcccccc cggcccaagg
    20221 tggttatgtc atttcgcaca ctcattcctt gaaagcctgt tacaaccaca accttgcctt
    20281 caagaagttc catcttgatt cttttggtat caatatcaaa tatatccgca cttccaaaaa
    20341 taccattggt gaagattccg gcctgaactg aatttaatgc gacggcatca atcccctgct
    20401 gtacaaggct atctgtgagc acgcatgcgg aaataacctc gccgcagctc atcatcaggt
    20461 ccaatacagc aggattgcag ccttttccta ataaggatat caaagtgtcc gtagcataag
    20521 gagaaccctt tcgacccatt gcggaaacca cagctattat actttttcct gcagtcttta
    20581 aagatgctat gtgtgctatg gcttcctttc tttccatttg tgttgccaga gatgtcccac
    20641 caaatttaac aacaactatc tccatgtaac cacccttaat ctgtataatt cctaaaaatt
    20701 tttatagtac attatattaa tggatactct gtactagccc aaaaaagtac ttacctgccc
    20761 atcttttgat tagcaggtaa gaatactatg caaaatttat ctaaaaatgt tatagtattg
    20821 taattatttt ataaaagctc ttttatgaga tcttctctat ttcttgaaga caaatagaaa
    20881 attggcacgt caaatactgt tttagccccc ttcattcctt ccatattcat tctataggca
    20941 gctctggcat atgaaaggag aatgcagcct gtaaattcag gattgctgtc caatttaagc
    21001 gagaattcaa ttgtatgatt gttttttcca ccgcctgttt tgccgtttct gattacaagt
    21061 cctccatggg gcatttttga atgttccttt ttaaattctt cctcacttat aaagttaacc
    21121 tttgtattgt aatcagcaaa ataatcaggc atactcacaa tatccgcttc aattttattt
    21181 ttgtccccgt cttcgctgca taccacgaaa cattctctca tatgtttatc cctgactgtt
    21241 aaatttgggg tttctcctct tctgacacgg tcaactgcac tttctgaagg tattgtatac
    21301 tgaataccat attttacaga atcaaccttt cttatggcat cagaatgccc ctggctgact
    21361 ccctttcccc aaaaggtata tgagctgcca ttcggaagaa ctgcctcaga aatcattctt
    21421 attatggaaa atagtccagg atcccatccg atggaaacta tgctgacttt acccgatgat
    21481 aaggcgcttt cattgatact tttcaagtat gcaggtattt ttgcatggtt gtcataagag
    21541 tcaactgtgt taaataactt tgcaaactga actccatgta gcggaagatc ttttgcagaa
    21601 cctgtacaca acacaatcac atcgataata tccttgtatt cttctgccaa agaaaaattt
    21661 accaccggta ctcccggact ttgaatcttt aattcatcag gatttctcct tgtaaagaca
    21721 gcggctaatt ctgtgtctga actgtctctt acggctgttt ctactcccct gcctatattt
    21781 ccatatcctg caatacctat tcttatcttc atagcctcct ccatatctgt taaacttaac
    21841 tgtttttatc attatattat ttttataggt atttatgtcc gttgaaacag gccttaataa
    21901 tgaatcctat attttagaag tttttaatgc ttttttaaga aagatatatt ataatgaata
    21961 caatcaatga agcaaagact ttggtctttg ccctaatgta ttcattgtat cggtgtgaag
    22021 caagccgatt ttgcggcttg cacttatgat ataatgaata caatcaatca atgaagcaaa
    22081 agggagtgtc cccaggaaag ggagatgaaa aatatgttgt gctctatatg caagaaaaat
    22141 gttgcggtaa tatatgtaaa taaaattatt gaaggtaaaa tgcagactac aggtttgtgc
    22201 attccttgtg ccaaaaagca aggtatagcg cctatggatc agataatgag acaaagcggc
    22261 atgaagcctg aagactttga gaacataaac aaccagctta ctgaaattat cggagatatg
    22321 gatttagaaa aattatcaca ggatatgccc cctgaaaaca gtgatggcat taattctctg
    22381 atgaatttta tgaataatgc ttttacaggc agcacaagct ctacaaacag cgagttcctt
    22441 ccctcggaag atgaacagaa tgctgactat gcaggcacgg ggtccggaac aggaccaaaa
    22501 ataaaggaaa aggtaaaaag aaaaaaactt aaatatcttg atacctatgg tactaatctt
    22561 actgaacagg cacggtttgg tgaattagac agaataatag gcagggagag agaaattgac
    22621 agggttgttc aaatacttaa cagacggtct aaaaacaatc cggttttaat cggtgagccg
    22681 ggtgtaggta aaactgccat agcggaagga ttagccgtaa gaattgctga gaagcaggtg
    22741 cctgtaaaac ttttcaacac tgaaatttac cttcttgact tgactgccgt tgtggctggc
    22801 acgcaatttc gaggccagtt tgaagcaagg atgaaggcaa ttattgatga ggccaaaaaa
    22861 gccggaaata ttgtaattgt aatagatgaa cttcataata ttgttggtgc gggggaagct
    22921 gaagggggag ctatgaatgc tgccaacata cttaagccgg cacttgcaaa gggtgaggtg
    22981 caaataatag gtgccaccac tttggatgaa tacagaaagc atgttgaaaa ggatactgcc
    23041 cttgaaagaa gattccagcc tgtaatggta gatgagcctt ccgtagaaga taccattgaa
    23101 attataaaag gaatcaagga ttactatgaa cattatcaca atgtaaagat aactgatgaa
    23161 attgtaaaac aggcggttat tatgtctgaa aggtatatta cagacaggtt tctgcctgat
    23221 aaagcaattg atgttataga tgaagcaggc tcaagggcaa acttgaaaaa caagggcctg
    23281 gttgagttgg aagctttaag gtcggagctt agcagaataa gggaagaaaa ggaagaagct
    23341 gctgaaaacg acgactatga aagagctgca gatttaaagg taaccgaaat cagaatcgaa
    23401 gcaaaattaa atgacttatc caaggaatac gaaaatattc ttttgactga agaggatatt
    23461 gcttatgtta ttgaagcctg gacaaaaatt ccggtgcaga aaataagcga agatgaagct
    23521 gataaattat tgcatctgga agaaaggctt cacagaagag tagtgggaca gcatcctgcg
    23581 gttacaagtc ttgcaaaggc aataagaaga aaccgttcag ggtttaagaa aaagaaaaaa
    23641 ccgtcttcat tcatattcgt aggacctacc ggcgtgggca agactgagct tgcaaggacc
    23701 ctggcttatg agctgtttga aaatgaggag gcaatgataa ggcttgatat gtcggaatat
    23761 atggaaaaac acactgtttc caagcttata ggagctcctc ccggatatgt gggttttgat
    23821 caggctgggt tcttaacgga gaaagtaaga agaaagccat attcggtaat attattggat
    23881 gaaattgaaa aagcccatga ggatgtattt aatatactgc tgcagatatt ggaagacgga
    23941 aggcttacag acaatcaggg aaggactgta aacttcgaaa atacagtagt aattatgact
    24001 tccaatgccg gtacgtcaat caaggctaac cggattggtt tcggaaacac ggattacgag
    24061 gcaatggaat ccaaaataaa ggatgccctg aggcagacat tcagacctga attcttaaac
    24121 cgtattgatg atataatagt tttcagtgaa ttaagcaggg aagaattaag cgaaatagtt
    24181 gacctgatgc ttaaagaggt taaaaaagat gctgaagaaa agaaaataac cataagtgtc
    24241 agtgataaaa tgaaggacca catacttgaa aagggatata atacaaagtt tggggcaaga
    24301 ccgctgagaa gggctatcca aaagtatgtt gaagacgaaa tctcagaagc atatttaaga
    24361 ggtgttttaa aggaaaattc ttcagttaat gtagatgtaa atgatgaagg aaaaaccgta
    24421 ttgacaataa tataagtttg gtaaggcatg gcagggggga cacactggtc cccctctttt
    24481 tgttatttgt ttaaaaatcc catccctagt accccgggac ctgagtgggt tccgataaca
    24541 ggtccgattt cattttcgat tatatttttt attttatatt tatcttcgat tgtttctctt
    24601 aaaagcttta attggttgta agcatttccg tgaaacaaga gcactgtttt gtcggataag
    24661 tcataattat tcttttctat ccattcatca aaccatttga cagttttatt tttgcctctt
    24721 agtttttcta tcactttaat ttccccgtct ttaattgcaa cgattggttt tatgtttaac
    24781 agagagccaa caaccgcctg cccttttgaa agtctcccgc ctttttcaag gtactttaat
    24841 gtgtcaagag cagctacaac aactatcttt tccttaagac gatttaatat ttcaactatt
    24901 tcttcgatgg ttttattttc ttttactaat tctattgctt ccaatataag tgcaaatgaa
    24961 ccaagacata cgcttcttgt gtctattatg tgaatatcgt cgctgcctat catattcctg
    25021 gccattcgag ccgaatcata tgttcctgaa aattccgaag caacaaaaag acccagcacc
    25081 ttatcccctt ccagcaatat ttcactgaat gtttcaacaa atgcccccgg tgaaaccaaa
    25141 gttgtagttg gaagcttatc tgcagctgca agtttttcaa aaaattcctt ggaagatagt
    25201 tcaattttgt ctaaatatga ttgcttttca aaattaacgg ttaatggaac cactttcaaa
    25261 ttgtattttt caattatttc atctgttaca tcagacgtgc tgtctattac gattctaata
    25321 ctcatattaa cctctttcta taatcaaata tttggactat aaaagtaatt gtgcgtaatt
    25381 atgggtacag tcttgtcccc tttggtaatt tatttctgtc ggtctaccat cctgcatatt
    25441 tatatttttc gctgaaaaat acatcaatgt ttgaaagcac cttgattaat acttcttctt
    25501 catggctgtc caactgctgc aatccgctta ttaccagatc cttatgaaac tcttcatggg
    25561 atttgaatgc ttctctgcct ttttctgtca gtcttatttt tactaccctt cggtcttctg
    25621 atgagcggcg cctttctaca tatcctttat tgataagatt gtctatggca gttgtcaatg
    25681 ttcctgaagt aacttttaca gctttggctg tatcagacat ggtgcgttcg tttgtcatac
    25741 caatgttgtc tattacatgc atttctgtta tggataaatc agaaaattcg cctatcttca
    25801 agcatttttc ttcaatgtcc aaaatgttgg caaaaagtcg tacaagtatt tcatttaccg
    25861 tttcatgatt actcatcaaa ccaccccaat tactttgata gccaaattat atttatatta
    25921 ttcctatttg tcaagcatta ttataatgaa ttgtatatct ccacaaaaaa tcttttgctt
    25981 cctcggcata gtcaatattt atacgtttgc ttattacggt ttcaaaaccc ttaaagccgt
    26041 catcttcaac ataaatttca tcaccggtca aatctattcc attgtggtct gttgtaattt
    26101 ttaaagcatt gcaaagtttt ccgggaccgt ttgaaatatt tcttttctga taatttgtaa
    26161 gttcattgag atttttgttg tatctgttaa tactcatttc ttcttcatta attaagggtt
    26221 cagccgctct tattaaaaca gcgctgcagg ttccttcctc ttcagtgacg aagttcagac
    26281 aataatacat gccataaata aaatacacat atgcatatcc cggaggtcca aacataatct
    26341 ttgtgcgggg tgttatcttc cctccataac catggcttgc cttgtctatt gcagctatat
    26401 atgcttcagt ttcaacaatt cttgatttta tgacaatatc atcgtatttt cttactaaga
    26461 ttttcccgat taagtcttcc cccacaatgc gggcatccct gttgtaaaaa tttctattca
    26521 gcttcataat aaatacgggg acattctatg tcccctgcct cagattaaat aatcttttga
    26581 aattctccct tgttttttct gataagtcat caactgataa atttcttatt ccagctattt
    26641 cttcagcaac gtattttaca tattttggtt cgtttctttt acctctgaag ggtgcaggtg
    26701 ttaaataagg cgaatctgtt tcaattaaaa gcctgtcaaa gggaacttcc tttgcaacca
    26761 gtttaggcac tcttgcattt ttatatgtaa caggtccacc cagggatatg taaaatccaa
    26821 gctttaaaaa ttctcttgac aactccacgc ttccggaata acagtgcatt atccccctga
    26881 caccgtcata atattcttcc ttcataatat tcaaaatatc ctcgtgagca tctctgtcat
    26941 gtacaatata tggaagatct aattcctttg ccagccgaat ctgctccctg aaccattttc
    27001 tctgagtgac tctgtctgaa ttgtcataat agtaatcaag acctatttcg cctatagcta
    27061 tcaccttgtt atttttcgcc atttctttga atatattcat actttcatca ctcatatact
    27121 tgctgtcatg aggatgacat cctacggctg cgtatataaa ggggtatttt tctgctatta
    27181 atacagcgtc ctttgatgat tttaaatctg caccgggatt tagcacagct tctatatcat
    27241 tgtcatacag tgaattaatt atttcatccc tgtctccttc aaatctatca tcatcaagat
    27301 gtgcatgact gtctataaat ttacctgcca cttttcttat ccttcttttt ggtatttctg
    27361 taagttttgg catattccct gaacttctcc aattgttctt catcattcaa aaatgtaaga
    27421 ttaatgctgg tttccttgtt catcatcata taaataattt tcacctttac tttatttttt
    27481 acatccggtt taagataatt gataccttta atattttccc atctaactag gtttgatctt
    27541 ttgtgtattc ccaatgttga aatcaatgtt ttcctggaca gtggcacata taatgcaata
    27601 accagcacgg gaagcactgc caaaaccatt atatcttctc ctgtatatgt ttttgtttcc
    27661 atcaatcccc taatcattat tccagttatc aatatcatta ctgccatcaa aagtctgctc
    27721 agcaaatgaa ctgttttatc atcctcattc aataaaagca ggacttccat gccttctttc
    27781 atatatttca tccgatttct ggtgttattg aataaaaagt ataatattac aaaaaatatc
    27841 agcgggtaga atactttagc agctgtcata tgtaatgcac ctctctttga attttctgat
    27901 ttagtttatc ataatttaaa ttatgtttca agatgcaaaa atgatggaat tcaaaacaaa
    27961 agctacctcg gaagaggtag cgcagatggg atgaatcaag aatgtttcct taaaaactcg
    28021 gcgggaccaa tgcccatacc gaaacagata tttcttctcc tgtattaatg tatttatggg
    28081 gaatcgtgct gtcaagatat atactgtctc cttcattcag gtcgtatact ttgctgcctg
    28141 atataactct cagggtcccc ttgattacta ctccgcattt ttcgccttca tgtgatatca
    28201 gctcagtttc aggtttctcg tttggattaa tttcaatcat cagcatttca atttgttttt
    28261 tcaggtcggg actaaggagt tcatatgttc tgcttccttt tccaataatt atcttttttc
    28321 tttcatcctt aagcacagct tgattaaggc cattatattc atcgaaaaag taattcatgg
    28381 ttacattaag ggcttttgaa attttccaca aggaagcaac cgaaggccct gtaatatctc
    28441 tttctatctg gcttattaat cccttgctca gccctgacat ttcagacagg ttctctatgc
    28501 tgattttctg cttttgcctt aatgatctta ttttatcacc taactccatt tcatccctgc
    28561 tctctgtata aaagtgaaat ttctttaata cattatagtg caaatcggtg catttatcaa
    28621 tatttaatta tagttttctc catccaggaa gagaaaaaat acattattgc agctattact
    28681 cctaaaacaa atacccccat cattaccagg tctagtttaa aggtttggct gccatacaat
    28741 atcaaatatc cgatccctgc tttcgaaaca aggaattctc ctactataac tccaatccag
    28801 gataaaccta tgttgacttt tataacgctt aaaatatttg gtatattggc aggaattatt
    28861 acctttttta aaatttgaaa cttatttgct cccattgttt tcaacagtgt aattttatct
    28921 gtctctacac cggcaaatcc cgtgtacaat gtcattatag tcacaacaat ggatatagat
    28981 gcagcagttg caattattcc tgaataaccc attcctaccc acaatataat tatgggagcc
    29041 agggctgttt taggaaggct gtttaaaata acaatgtaag ggtccagtat cctggctagc
    29101 tttctaaacc accacaaaag tattgcaatc aataagcctc ccagggtacc cgcgaaaaat
    29161 ccgcctatag tctccattgt tgaaatgtat aaatgtttaa acaggcttcc atcaccaaca
    29221 aatttaacaa tcagttcaaa aattgctccc ggactgcttg taagaaatgt atcaatcaga
    29281 ttatttctgg ctgctgtttc ccataatatt attccaaaaa caaaaattaa aacctgagta
    29341 ttccttatga atcttttttc cttatttatt gactttatat attcatcatg ctctttcgaa
    29401 atgaatgcaa cctgtttatt tttctttttc cacatctaaa tccctccata tatttctgaa
    29461 atactctcca aaaaccaagg tgtttctgca tttcattggc gttctttcac ccacgcactc
    29521 ctcaaattct atattatatt cctttttaat attagcgggt ctttcagtga aaactataac
    29581 tctgtcagaa atactaacag cttcgggtat gtcatgagtc acaagtattg tgctgatttt
    29641 ttcatttctt aaaattgttc ccacttcatc accaatttct agtcttgtct gatagtccaa
    29701 tgcagaaaaa ggttcatcca ataacaatag gtcaggctca attgccaaag tcctgaccaa
    29761 tgcggccctt tgcctcattc ctcctgacag ctggctggga taatggtttt taaaattcgt
    29821 caatccatat ttttcaagca tttttttcac ttttatctta ttttcttcat tatccatctt
    29881 ccttacttcc aagccgagaa gacagttttt atagacagtc ctccattcat ataactggtc
    29941 cttttgaaac ataaagccga tacttttggt attaacaatt acttcccctt cagagggttt
    30001 ttcaaggccg gctataaggg acagaaccgt tgattttccc gagccggaag gtccaactat
    30061 agaaactatc tcacctttct taaccttaaa agaaatatcg tcaattgcct ttgtctcgtt
    30121 atttattgta tgaaatcttt tagagatatt tttaagctca caaatatatt ccatccttac
    30181 ctccattaaa ctgtaactct ttacactatg ttattcaaaa gaggtaaata agttaatgat
    30241 aaatgaaata aataggtatg gggacatatc tctgccattg taataacctc tgatggcaga
    30301 ggaatgtccc aaagcagtga taatccgtga gcctgtctaa actgcttaac tatcgaaatc
    30361 agtgtaattc aaagaaggga tatttaaaat actaaggcgt tcatgggaca ggacagttaa
    30421 aggtatgtca ccatgctttg cagtctttta aaaatttcgt aagtttattt aacctgccta
    30481 aaacttcttt atttactgca tgttcaatta tttcagtctg ttccaaaatg ttttccttta
    30541 ctcctatgca ttccagaaac tcaaagatta tcctgtgcct ttccagcaat ctgccgcctn
    30601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc atattatggt ttaagaatgt
    30721 tagcttaggc taaattatcc taaaaaattc tatgcaatta aattttaaat attacaggag
    30781 cttggtatga aattagttga tgttggtgtt ggaaaaacag taaaggttga gcagaactca
    30841 gccagggatt atttaaagga aagatttctt gctttagggc ttacaaaagg tgcagatgtg
    30901 gaagtcttaa gaaagggtcc aaaaaataat ctaaccgttt acaggataag gggggcaatg
    30961 attgccctga gaaacgaaga atcaaggttg attgaggttt tgtaaggggg cggcatcatg
    31021 ggacttacag cacaatcagt aaggttggaa tcattaaaag atatttatga aatagaaaaa
    31081 aaagataaac agtatataat agccttggca ggcaacccaa atacaggaaa aagtacagtc
    31141 ttcaattatt tgacgggact taaccagcat actggaaact ggcccggcaa aacagtagtc
    31201 tctgcaaggg gacagtttaa atacaatgaa aatgaatata taatggtaga cctcccaggg
    31261 tcctattcat tatttaccgc ttctaaagat gaagaagtta ccagggattt tttatgtcac
    31321 ggcaattatg atgcagtggt tgtggttgca gacgccacat gccttgaaag aaatttaaac
    31381 ctggccttcc aggtatcgga gcttacaaac aggataatcc tttgcataaa ccttatagat
    31441 gaagctagga aaaaaaatat tataatagac aaagaaggtt tggaaaatga actgggcata
    31501 ccccttgtct taacttctgc cagaagcggc cagggcatgg atgagttaaa aaatttaatt
    31561 gaaaaaacag cacataaaag caccgtggat ggagctgaag aattgctata ttttaatcag
    31621 gatgaagaaa taagagaaaa aatttctgag gataattata attatgccaa cagattaaaa
    31681 caaaaatatg taaaggagga tattaacaag cttaacaggg acaaaaaaat tgatgatatc
    31741 ataacatcaa aaaagttcgg catacctatt atgcttgctc tgctggggct gattttttgg
    31801 attacaatta caggggcaaa tatgccctca cagattttag ctgatatatt gtttgttttc
    31861 cagtataaat tggatgctgc ttttatcagc ttgggagcaa atgattttat taggggtatt
    31921 ttagttgatg gaatgtaccg cagtcttgcc tgggttgttt cggtaatgct tccccctatg
    31981 gcgatatttt tcccgctttt tactcttttt gaagacttgg ggtatcttcc aagagttgct
    32041 tttaatttgg accatatgtt taaaaaagca ggcgcccacg ggaaaatgtg cctcaccatg
    32101 tgtatgggat tcgggtgcaa tgctgcagga gttataggat gcagaattat agactctcca
    32161 agagaaagat taatagccat cataaccaat aaccttgttc cctgcaacgg aaggtttcct
    32221 ctgataatag ttttatcggc tgcatttttt gcatttacag gcacatttct tgacagcatc
    32281 atacctgctt tgtttattac tttgatggtt cttatgggtg tttttgcaac cttgactgta
    32341 tcctttttat tgtctaaaac gattttaaaa ggaattcctt ctacatttac actggaactt
    32401 cctccctaca gggcacccca ggcaggaaga ataatttata catcattgtt agacagaaca
    32461 atatttgtat tgtcaagagc agttattgtt gcagcacctg ccggagctgc tacatggctt
    32521 ttggcaaata tttatgtgga cgatatgagt atattgattc atgcagctaa ttatctggag
    32581 cctttgggaa gagccatagg ccttgacgga ttcatactta ttgcttttat agcaggtttt
    32641 cctgcaaatg aaatagtgat tccggtttta ttgatggcat atatgtcaac aggatctttg
    32701 acagattttg aaagtattag tttattaagg caaatactat tatccaacgg ctggactgca
    32761 ttaacagcca ttaatgtaat gttgttttcc cttcttcatt ggccgtgttc cacaacattg
    32821 tggacaataa aaaaagaaac ctgcagtctg aaatggacat ttgcagcctt cataataccg
    32881 actgcccttg catttttggc atgctttgca actgcaacag tttacaggtt aattttgtaa
    32941 tgattttctc ccataaaggc taaatcataa gacatagcag caggggcatc agtccaattt
    33001 tgcctaaaac aagtagtgca gtttaaaatg cagtgctcgg taatttccag atacaccttt
    33061 ctaaggtcat tttgctgcat cttactctcc tttatacccg gcatctatcc gcctcctacc
    33121 ggaggaaaga tggcgataac atcgccatct ttaatcggtg catctaaatt agaatgtctg
    33181 ccgtttatta ggtatatggc aacatcttca ggacatattg ataaatgttt aagaacatca
    33241 ccggcaatgg taaaagagtt tgggtcaagc ttaataattt tttccctgcc atcacgtagt
    33301 gttgcaaata aacgtatttc aatcatttat tccaccagcg cttcttcttt aagcatttct
    33361 tcttccaatc ccagcctcat taatgtttca tttgttggga atgcatcaac ccagccccga
    33421 acattgtaat aatccggcaa ggtaacagag attttactta attcaccttt tgacgggcct
    33481 tcaggaatag gttcttcagt taaacgtttt ggcaaccggt catcttccgg cttcatacct
    33541 gctaatttgt tgaataatct ttcaatattg taaatacgct cgccaacttc caataatctt
    33601 tcagctgtat aatcagttcc cgttgcagca ttcagcaatg cagcatattc cggggctccc
    33661 aatgcaaagg aagtaaataa gcacataccc attgaatcaa tacatgcagt taaatcctgg
    33721 aaagttttag cccattttgc ttttccttct gtggtgaaac gatccagtgc ttcaggcaat
    33781 cccaaaactt cgggagaaat catatatccc cgtacatggc agccgcctct gttgctggtt
    33841 gcatatgtga tgcctattcc ttgaattcct cttgcatcat aagcaggcat ttcttgtttt
    33901 ttaacactca ttgaatattc cggcacacca taatgttcac aaagacgata tgagcccttt
    33961 gacattaatt ttgccaattc attatatccc tgacccattc gtttggtcca ttcaactatt
    34021 gcctttccgc ttccccagtt taaaggagcc ccgtcgcaat cttcatctgt aatatatccc
    34081 ctttgataca gctccattgc tgcagctatg gtacaaggag cagaaatagc atctaagccg
    34141 tattcattac acatatagtt tgcttcattt atagccggta aatcatctac accgcagttt
    34201 gcgccataag cccatatagg ttcatattca ggtccgccga taattttgcc cttaaaatct
    34261 attacacggc cacagcctat gggacatctg tgacagaagc cttgcttcac taaatgattt
    34321 tcctttaatg tttcgcctga aattttatca gatgtttcaa aatatgattt ttgccagttt
    34381 tgtgttggaa aagagcctaa attatttaca atattcacca gtaccgatgt tccataagta
    34441 gggagccctc cgctggtaac accgttttct cttatttttt tctgactatc tgatgcagcc
    34501 tgccgcaaca tatcagtatc aaaaggctcg ataacagtac gggaagctgt aactgaaatt
    34561 gctttcaagt ttttcgaccc cattactgct ccaaccccgc ttcgtcctgc tgccctatct
    34621 ttatcgttca taaccgcagc aatcagagat aatttttcgc cggcagggcc aatatttaat
    34681 atagaagctt tcgggtacaa cttaagcatt tcttcaacta catcttccgt agttttcccc
    34741 cagtattcgt ttgcaggcaa aatctctgct ttttcatcat cgatattgat atacaccggt
    34801 tcctttgctt ttccttccac aataatcgcg tcccagccgg catacttcaa ttttgcaccc
    34861 catacaccac cggaattgga tgatgcgatc atcccgctaa gcggtgattt agtaactacc
    34921 atgtagcgtc ctcctgtagg gacacttgtt cctgtcatag gaccgttaat gaaaactagt
    34981 ttattatcct ctgataaggg atcaacagtt gcaacgcctt catccaacaa aaattttgtt
    35041 cctaatccgc gtcctcctat aaatttcttt gcaacgtcca tgttgagttc ttccggttta
    35101 acctcacctg ttgttagatt aacccttaaa attttaccat tgtaagcagt tgtcatcttc
    35161 ttccatcctt tcattagaat taaaaaacaa ggtgcttcac gcaccttgta tataaactaa
    35221 taaaggcacg cgtgaaaaca cggtaccttt ccaaacacgg gaggcattaa ttattattaa
    35281 cacccattgg tttctttaag aaactcggtc catttatatt ataccaaaga attggtaaaa
    35341 atggaagaaa aagttaatta gaacggaaga aaaaattaat taaaattagg aaaaaagtat
    35401 taaaatgact tttttattct tcttctgacc ttttcttctc ccattatctg ctgcagctcc
    35461 cataggtcag gtgatgatgc ccggcccata agagcaattc ttattacggt gcttacatct
    35521 cccacatgac ctttgtaaat atcgggattt ttcttgtaat ccttgggctt tgcagcatat
    35581 ccgttttcag atgcaattac tcttattttt tcaaaccact gactttggtc gtcagagtgg
    35641 tcgtaggtat ttaagtaagc ttgaaggagt tttttggctt cttccacacc gatattttca
    35701 ggataagaat caacaatctt gaaatactca tcaaagtaat agcttatgaa gtcaaagatt
    35761 tgttcacaat acatcaaatc cttccgggga ttattccctt ctcttcccac agacaagagc
    35821 tttattacct ggtctttatt tgatgacaaa agctcaaagg cttccttttt atactcattt
    35881 aaccagctgt gaagaaattc ataaatttct tctgcaggga tttttaaaag tacatccttg
    35941 ctgatgttgt ttagcttgtc taaatcaaac aaggctcctg aattgctcat cttttccaat
    36001 gtgaattgaa aatcatcaat aggtctgtcc ggattttcca tcctccattc ttcaaaatta
    36061 gaatttaaaa tggtaataag atattccctt acagccttag gatgatagcc taattccatg
    36121 taatatccca atcctaattc agggtccttc ctctttgaaa gttttctctt ggtaccgtca
    36181 tcaattttca tcaaatgtgc tgtatggcaa taaacaggtg tttcccagcc aagtgcttta
    36241 aataattcat aatgaatggg aagagttgaa agccattctt ctccccttac aacatgagtt
    36301 atcctcatca gatggtcatc aaccacatga gcaaaatgat atgtaggtat accattggtt
    36361 tttaaaagaa caatatcctg aaaattttca tggattgaca aatgacctct aatggcatcg
    36421 tctatctcaa attttccttc ttctgttccc tcagaacgga aacgcacaac aaattcttca
    36481 ttattatcta aatgttctcg gacttcctca agacttaaat ttctgtctct tgcccacttg
    36541 ccgtaataac cggtatttac attttcacct atctgagcct tccttaagtc ttccagttct
    36601 tcttcagagc aaaagcatgg ataggctctc cctgtttcaa tcaggtgttt ggcatagcac
    36661 tgataaattt ctgccctgct gctttgaaag tatggtccat aagctcctct ttccccttca
    36721 attccggcac cctcgtcaaa ttttaagccg aaatattcaa gggatgaaat tattgtttca
    36781 actgagcctt caacctttcg tttctcgtct gtatcttcga ttctaagtaa aaaaacaccg
    36841 ccgctttggt gggctaaccg ttcatcaaca aatgctccgt aaagattgcc caaatgaata
    36901 aaaccggtag gactgggtgc tagtcttgtt actttagcac cttcaggcag gtcgcgcttt
    36961 ggatatgctt cattttcata atactcaatt gtctttgtta tatttggata taatagttcc
    37021 gccagttttt taaaatccat tttatcctcc attgttcaaa gggacggggt ttgtcatatg
    37081 taacaacaac cccgtcccct tatattattg gggacattcc ttctaccctc aaagactatc
    37141 cctttagaga aggtgtgtcc cctttcctca ataacccata ggggagtcga acccctgatt
    37201 ccaccgtgag agggtggcgt cttgaccact tgaccaatga gtcttattaa gaacattctt
    37261 tactcttaaa gactatccct taagagaagg tgtgtccccc tacctctagc attatataac
    37321 aagaccctgc ctttgtctat atttttttat ttttctgaac tgttgccgca gcaaccgccc
    37381 acatctcctt ctttaaccat cagtgtttct tcctctgatg cttttataaa tcctatgttt
    37441 tccaatgttt tttccaggtt ttcatctgct tccatgtatg tttttccggc accgattgta
    37501 actgcctttc ttacaagcag ccttacaagc aaatcctcat agtttaaacc cttgaattgc
    37561 tctttaagac acagcttgtc aataaagtat ttgccttctt taaatagaag ccttccagtc
    37621 cctgccggta aatcatcctc atacaaaaca gcattaaagg caaattcatc ataaaaatca
    37681 gtaagcttat ctgcctctaa aagtacttct ttccttatat caagcacaac acttaaatta
    37741 tctataccct gaaaccatct tgttgaaatc attatccacc tcacccttat tgttggtgtc
    37801 agcaccacaa agtaaatata ttataccata cttttgcttt aataacataa gcattattag
    37861 ataacaacga gatatttatt tctttccatt actgttatta tttctttacc tttaagcagt
    37921 atgtgatata attgttttag ctttgaatat ggaggtgtcc tgtgttaggt gaaagattaa
    37981 ggcaaataag aaaagaaaac aatttgacaa agcaagaact tagccttgca ttaaatgttc
    38041 cgaaggaaag aataaagctt tatgaaacag gggatgaaat tccttctgat ttttatttgt
    38101 caagttttgt acaatatttc aatatttcaa aagaagaact gattgaatta attaaataaa
    38161 tttaacaatt ataatggttg aaataatgat tgttatatgt taaattaata acatgtctaa
    38221 gcttaaggag cttctaagag caaggaatat aataatatta aaagtattat tccttgtggt
    38281 acaaggaatt tttttattga ggtgtgtatg gataaaagaa tcggcgttat aggtattgta
    38341 attgaagata tgtcccaggc agcagaagtg aacaatatcc tgcatgagta ttctcatatt
    38401 attataggaa gaatgggtat accttacagg gagaggggtc ttaatgttat ctctctcatc
    38461 gtcgacggaa ctaatgacga tatcggtgct cttagcggaa aattaggcaa aataaaagga
    38521 ctcagcgtca aaagcgcttt gtcaaaaaaa cattaggagg caatatgaaa aaaatcagtt
    38581 ttttattggc agttgtttt
//
LOCUS       DAOPUT010000007        36250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_1110_length_36250_cov_18.784805, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000007 DAOPUT010000000
VERSION     DAOPUT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 36250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36250
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_36250_cov_18.784805"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..424
                     /locus_tag="PKK75_01580"
                     /pseudo
     CDS             119..424
                     /locus_tag="PKK75_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
     misc_binding    612..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1036..3447
                     /gene="leuS"
                     /locus_tag="PKK75_01585"
     CDS             1036..3447
                     /gene="leuS"
                     /locus_tag="PKK75_01585"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNZ82316.1"
                     /translation="MKSYNPQAIEKKWQEIWRKNETFKAKDNTDQKKFYGLVEFPYPS
                     GVGLHVGHIRAYTSIEVISRKRRLEGYNVLFPIGWDAFGLPTENYAIQTGKHPRKVTD
                     DNIRIFTAQLNAAGYSFDWSRSVDTTDPEYYKWTQWIFVQLFKHGLAYKDKTYVNFCN
                     SCRVVLANEDSQNGICDRCGSQVVQMEKDVWFLRIRDYADKLLDGLNEVNYLPRIRLE
                     QENWIGRSYGAEVDFKLKDTEDNLRVFTTRPDTLYGVTFMVMAPEHPFLKKYEHKIRN
                     MDQIKEYQEETKKKTEFERVQLAKSKTGVEIKGLTVINPVNKEEVPVWVADYVMMGYG
                     TGAIMGVPGHDQRDWDFAKKYNIPIIEVIGGGNVKEEAFTDVEDGILVNSGMINGLRV
                     KDAIAKITDYLVENNFGEEKVNYKMKDWAFNRQRYWGEPIPIIHCPHCGMVNVPEDEL
                     PLLLPDVENYQPTDSGESPLANIPEWVNVKCPVCGEDAKRETDTMPQWAGSSWYFLRY
                     IDPHNKDAIASREKFDYWGPIDWYNGGMEHVTRHLIYSRFWHRFLYDIGAVPFKEPYA
                     KRTAQGLILGADGEKMSKSRGNVVNPNDIVDEYGADTLRTYIMFIGDYEKYAIWNDAS
                     VKGCKRFLDRTWKMQEMKSFDKGYSEKHMTLMHKTIKKVSEDFEAMKFNTAIAAMMTF
                     VNEIFNDGYITGDELKTFITLLYPVAPHIAEEIWQEQGFEGMLNQTKWPSWDEEKTIE
                     DIIELPVQISGKVRGKIELPKDADLNTVRELTQADENILKYLEGKTIIKEIYIPGKIF
                     NLVVK"
     assembly_gap    3870..3969
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3970..4586
                     /locus_tag="PKK75_01590"
     CDS             <3970..4586
                     /locus_tag="PKK75_01590"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HNZ82317.1"
                     /translation="MMASCFCKKSYVRVLNTFNMPLDIQVNDILMGENLQSGEFTRYV
                     KFPPSSYHVVMHAKGDLVFESDIDIDNNLTYTAVVSADDEDISDICILMVPEKKVHHM
                     GSNMSALRLANLCPDEPEVVLSASDGTVLFSDIKYGKVSCNVAVPSGRYILHLRKPDG
                     TEILEHSMDAAPYMHYTLFAAGSQNDNSVKIIVPEDGVNYLDLC"
     gene            4671..5366
                     /locus_tag="PKK75_01595"
     CDS             4671..5366
                     /locus_tag="PKK75_01595"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HNZ82318.1"
                     /translation="MKTIGIIGGMGPLATVHLFERIVLRTKAEKDQDHIRVLIDSNTN
                     IQDRTKAILGIGESPVKELVNSAKILENSGADFLIMPCNTAHYFIDEIKKNINIPFIN
                     MPDETVKYTYDKYGKDALVGIMATDGTIKTKIYENYYAGLGIKTLVPIKTQGKIMEFI
                     YDVIKKGNYDAGTDLFFECVNELKDLGATAFLLGCTELSSAQYLYKFEGSFINPMEVL
                     AERAIIFAGGQLN"
     gene            5581..6402
                     /gene="purR"
                     /locus_tag="PKK75_01600"
     CDS             5581..6402
                     /gene="purR"
                     /locus_tag="PKK75_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HNZ82319.1"
                     /translation="MKKYKRNQRIGALMKIFAEKPNYLFSYNYFCEIFNAAKSTISED
                     VAIVKDLAEEMNFGRIETISGASGGVLFIPRLGNKERNDILEHLCSLFSQKNRILPGG
                     FIYMTDIFNNPAIVSNIAKVISSEFNNRHIDYIVTVETKGIPVAIITGQRLNVPVAVI
                     RRNNKITEGATVSINYVSGSSNNIQTMSLSKRSLKEYSKILIVDDFMKGGGTCKGIED
                     MMREFNADVVGTAVVIETMEPEKKLVDNYTSLLVLNNMSLKEGIIEIHPNTKLLK"
     gene            6510..6803
                     /gene="spoVG"
                     /locus_tag="PKK75_01605"
     CDS             6510..6803
                     /gene="spoVG"
                     /locus_tag="PKK75_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HNZ82320.1"
                     /translation="MKISDVRVRKINSEGKMKAIVSVTFDDCFVVHDIKIIEGQEGLF
                     IAMPSRKMPDGEFKDIAHPINSETRNLVSEAVFRAYEEKRTEESENVEEAALL"
     gene            7128..8498
                     /gene="glmU"
                     /locus_tag="PKK75_01610"
     CDS             7128..8498
                     /gene="glmU"
                     /locus_tag="PKK75_01610"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNZ82321.1"
                     /translation="MNMSIILAAGEGTRMKSDIPKTLHKVCGKEMIKYIIEASVRSGI
                     EKNIVVLGHGKEKVGHNIKEMNVQTVEQPIGESFPYGTGYAVMAAENHIDDVDTVLIL
                     CGDGPLIRKETLTGFMNYHFENDYAATVMTAKLDNPKGYGRIVRNRDGSIDKIVEEKD
                     ASEEIKNIKEINTGIFCFKGHLLKDALKKITRDNSKNEYYLTDTIKVLNNQGTKTGGW
                     LLEDADEIKAVNDRVQLAEVNKIMQRRINQRHMYSGVTIVDPDNAYIDDTVIIGRDTV
                     IYPNTYLEEGTVIGDNCKIGPNSRIINSVLCNNVTVENSKMIESYADDGTLIGPFAFL
                     RPGSRLGKNVKIGDFVEVKNSIIGDNSKSSHLSYIGDAEVGKHANIGCGVVFVNYDGK
                     NKYKTIVGDGAFVGSNSNLVAPVTIEEGGYVAAGSTITENVKKGDLAIARARQVNKEG
                     KGSNRY"
     gene            8509..9081
                     /gene="pth"
                     /locus_tag="PKK75_01615"
     CDS             8509..9081
                     /gene="pth"
                     /locus_tag="PKK75_01615"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNZ82322.1"
                     /translation="MFIIAGLGNPGKEYTGSRHNSGYMTVEHLAQRLNVKLNKLKFNS
                     VYGETYIDGEKVMLVKPVTYMNRSGIAIGEIVKFYKLPTENIIVIYDDIDIPLGTLRI
                     RPNGSPGTHNGMKSVIESIGSSFPRIRIGIGRNEDMDLADYVLQSFSQDEKAVVSNII
                     EKAAEAALEIIENSVESAMQKYNVKGLSES"
     gene            9238..12645
                     /gene="mfd"
                     /locus_tag="PKK75_01620"
     CDS             9238..12645
                     /gene="mfd"
                     /locus_tag="PKK75_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNZ82323.1"
                     /translation="MACNLLDISDKVLIITSSEAKSKKYEEYIKAYTDHSNRLQPKEF
                     ILYNVDALSKDVDHKRSDLLHDILNRKKNIVTASINSIITRVMSKDRFKESIIELKYG
                     LSYDMNELRSRLIKLKYERVDAIEGVGQFSVRGGIIDIFSPSQANPCRVEFFDDEVDS
                     IRLIDLKTQRSVKNVGSVKILPCSDVLFKKEEIKTILAEVEKDYNLRQNKNLGREAAK
                     NLKNLYNSYQDKLMSGMGIENADLLVAFTKESFSSILDYFGDDELILLVDEPERIFEE
                     LRTINESFRLKYSELFEKGEIFSNQSQVFISEDELLEEILKRPFISLNGKEKAFKTDT
                     SVHMLFKEAPSYYGRIEELSKDLNRLKYKGYKVNIVLAGYESCKKLYNLLNDYECSAI
                     LSRDANTAESGQVVITPGELTKGFEYYDNKILVLTENEVLGSARRKSRKAVKRKGAAI
                     DVFTDLKAGDYVVHEHHGIGQYVGIEKIKVQNIQKDYLCIRYKAEDRLYVPVDQMSLI
                     QKYIGSDSEKPKLNKMGSSDWAKTKERSKAAIENMAADLIKLYAERKVSKGFAFSGDT
                     EWQKEFEYKFPFEETEDQLRCVKEIKKDMEKPICMDRLLCGDVGFGKTEVAMRAAFKA
                     VTDSKQVAMLVPTTILAQQHYTNLVERFRGYPIRIEMLSRFRTHYQQNKIINELNKGL
                     ADIVIGTHKLLSKDLKFKDLGLLIIDEEQRFGVRHKELIKQLKSNIDVLTLSATPIPR
                     TLHMSMIGVRDMSIISEPPGDRLPIQTYVIEYNEGLIKDAIEKEILRKGQVYYVHNRV
                     ADIDRAAAKLRRILPDARIAVAHGQMSERHLENVMFEFVNREYDILVCTTIIETGMDI
                     PNVNTLIIDNADYLGLSQLYQLRGRIGRSNKVSYAYLTYEKDKMLSEVADKRLKAIKE
                     FTEFGSGFKIAMRDLEIRGSGNILGQEQHGHMLAIGYDLYVKFLERAVREIQGKQLEE
                     DIETSVDLNVDGYIPSSFIENEEQKIEIYKKIAAAENKNDILDITEEIIDRFGNIPPQ
                     VGNLLKISYIKSLCKRLHIKAISQAGNTVSIEMTGTSDLGQNIIEFLIKNYYKKISFV
                     GTNEPIVKYRLDSLEQEKLLKELEGFFEILNNFKADTAAVEEKHEQI"
     gene            12632..13573
                     /locus_tag="PKK75_01625"
     CDS             12632..13573
                     /locus_tag="PKK75_01625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ82324.1"
                     /translation="MNKFKKSLFLITLMCIALVFTACSDKESQDAVEGVMATVNGKAI
                     MQKDYDDAIAYYKSYVEYQFGEDAWEKEAARGMTYKEIYEEYVMNEMTNSLLLLDAAE
                     KEKIEVTEEDKQHALENFKINFENDEDYKSFLEQRNMTEEFVLGELTKEILVNNYITT
                     KIDSLLPGEDELKTLFDEMKMNTQVKASHILVNTEEEAIKAIERINKGEDFAEIAREL
                     SIDPGSGPNGGDLDYFNYAQMVKPFSEAAFALEIGEVSKPVKSDYGYHIIKLTDKIVD
                     EDITVESEKDKLIEYYKTNRYQDLLEELKSNAVIVNN"
     gene            13769..14320
                     /gene="spoVT"
                     /locus_tag="PKK75_01630"
     CDS             13769..14320
                     /gene="spoVT"
                     /locus_tag="PKK75_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HNZ82325.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDREGEI
                     ILKKYSPIGELTEFAGEYVESLFETTRHIALITDRDGVIAVSGSSKKDYTEKRLSPEL
                     EKIIESREIYQTDATSKPIRITANEFNPDNYTSQVISPIMVHGDPIGAVILLSKDKGS
                     KMSEVEEKLIKTASIFLSRQMEN"
     gene            14611..16266
                     /locus_tag="PKK75_01635"
     CDS             14611..16266
                     /locus_tag="PKK75_01635"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ82326.1"
                     /translation="MKIRFLGAVNGVTGSNHLIQASGRNIMLDCGMYQGKDEDLNAEE
                     FAVNPAEIDCLLLSHSHIDHSGRIPLLIKKGFKGNIYCSKPAYDLCEIMLLDSAHIQE
                     TEAEWENNKAKRSGKEFVEPLYTQEDALKSLQYFKPVLYDQIINIDENITVRFNDAGH
                     ILGSSIIEIWIKDEEDTIKLVYSGDLGMHEKPLLNDPVQINKADYLIMEATYGNRIHE
                     NINERTGELINIILKTARRGGSVIIPSFAVGRTQELIYELNKYYDSHLNEIGTEENEL
                     RKIPVYIDSPLAVKATEVFKRNSYAFDKEAKEHIMKGDNPLDFQNLHFAVTADESKQL
                     NFSKEPKIIISASGMCEAGRIRHHLKHNLWRKEASIVFVGYQAEGTLGRRLLDGEKNV
                     KILGEEIKVNAEIYNIEGFSGHADKPALLNWIDGFKEKPGKIFIVHGEKEAKEELAME
                     VKERFNINSIIPEYNILYDIKEKNFVNEIVLESKKQKIEDKHINELLKEFEELKKVFE
                     NALINTKSYLNDEDIDIDKIKNINNNIKSLENEIMNLTIISAK"
     gene            16291..18063
                     /gene="iorA"
                     /locus_tag="PKK75_01640"
     CDS             16291..18063
                     /gene="iorA"
                     /locus_tag="PKK75_01640"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNZ82327.1"
                     /translation="MKVLMTGNEAIAQGAYEAGVHFAAAYPGTPSTEILENIAPHKEI
                     ISEWAPNEKVAVESAIGASFAGARALTAMKSVGLNVAADPLFTVGYTGVNGGLVLVNA
                     DDPGLHSSQNEQDNRYYARMAKVAMLEPSNSQDCKDMVMKAMDISEKYDVLVMIRMTT
                     RVSHSKSIVETGERVEVPVKAYVKNPQKNIMAPANAKAAHPVVEKKLKELEKFSNETD
                     LNYIEWNNKKVGVISAGAAYQYAKEVFGKDASYLKLGFTFPLPMEKIKKFAEEVKTLY
                     VIEELEPYMEEQIKAAGISCIGKEKITNIWELNPDIIKKSIFGRENEVIEYDKTIAVG
                     RPPTLCAGCPHRAFYYQLSKKKNTVITGDIGCYTLGGSPPLNSIDTCVCMGASISMAH
                     GAKKIFEKNNDSKRVVATIGDSTFFHSGMTSLLDVVFNQSNTITCILDNRITAMTGHQ
                     ENPGTGYTLQGEITDTVQIEPVVRALGIKNVKTVNPIDQAAIKDALNWAFDLDEPSVI
                     ISRYPCALKKYSKEDIEEFGPISGYCQVDEDTCIGCKICVGTGCPAIVFKKEIKKSSI
                     NKVQCVGCELCLQLCPKKAIKVGE"
     gene            18065..18640
                     /locus_tag="PKK75_01645"
     CDS             18065..18640
                     /locus_tag="PKK75_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNZ82328.1"
                     /translation="MKDTKNILLVGVGGQGTILVSKILSTGLMEAGYDVKMSEIHGMA
                     QRGGSVSTQVRYGSEVASPIIGIGEADILVSFETMEALRWLEFLKPDGVVVINDFQIA
                     SAPILMGKADYPEGLIELIKGKAKTKVINAGEIAEELGNQKVMNVVLLGALIKSMQLT
                     DIDWEAVIRNQVKEKFVDINIKAFNKGMEAV"
     gene            18655..19722
                     /gene="buk"
                     /locus_tag="PKK75_01650"
     CDS             18655..19722
                     /gene="buk"
                     /locus_tag="PKK75_01650"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNZ82329.1"
                     /translation="MSYKIFVVNTGSTSTKIALYEDDKEVFVKSLSHPDELISKYDDV
                     QDQLHMRLEFVRSYLEEKKFDVKDLSCVVARGGMLLPVKSGAYAVNELMLDRVENRPV
                     MEHASNLAAPIAYDIAREAGVPSYIYDSVMTDEFPDIARISGMPDIPRNSTSHVLNTR
                     AMAIKYAKSIGKKYEDLNIIVAHLGGGISINIHHKGQIVDLLSDDEGPFSPERAGRVP
                     CRDLINLCYSGKFDKKTMQRKLRGNGGLKAYLNTIDARQVETMIQEGNEYAKLIYEAM
                     AYQIAKGIGDLATVVEGKVDAIILTGGIAYSKMLTSWIKNRVSFISHVEIMPGENEME
                     ALAYGALRVMRGEEKAREYTE"
     gene            20040..23762
                     /locus_tag="PKK75_01655"
     CDS             20040..23762
                     /locus_tag="PKK75_01655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNZ82330.1"
                     /translation="MNFDVKRIFVEKRPGFNTESLNLLNSLKEGLNIDKLSSIRILNR
                     YDVSNIDREDFENIVKTVFSEPNQDTVYYEDAGLKGNYFAVEYLPGQYDQRADFAAQC
                     AQIVTGKEKPLVKYARVFVLEGGLSDKDVEKIKNYCINPVDSREAEIAKPISLSHEQP
                     SSKDVETVEIINKSPKEIKKYKNDAGLAMSFEDLLFCREYFKNTEKRNPTITEIKVID
                     TYWSDHCRHTTFSTVIENIEFEKGRITEEIKSTYDEYLNTQKFVYESKRDVTLMDLAT
                     IGAKELRKKGLLNDLEISEEINACSIIINVDIDGKNEEWLLMFKNETHNHPTEIEPFG
                     GAATCLGGAIRDPLSGRSYVYQAMRITGCADPRQKIEDTLQGKLPQRKITTEAAAGYS
                     SYGNQIGLATGYVNEVYHPDYTAKRMELGAVIAAAPRENVKRESPSMGDLVILLGGRT
                     GRDGIGGATGSSVEHTEESIVLSGSEVQKGNAPEERKIQRLFRKKEVSTLIKKCNDFG
                     AGGVSVAIGELADGLNINLDLVPKKYEGLDGTELAISESQERMAVVVRKEDAEKFNHY
                     AHEENLQSTVVAEVTDNRRLKMTWRGKTILDISRDFLDTNGVRQKTSAKISEPLNIKY
                     ERKLNNNNINELWLENLKDLNVASQKGLVERFDSTVGAGSVIVPMGGRTQLTPAEGMI
                     AKIPVEEGVTSTCSLMAHGFDPYLSKESPYHGAIYAVIHSVAKIVAMGGDYNNIRLTF
                     QEYFEKLNKEPERWGKPLSALLGAYKVQHEMEIPSIGGKDSMSGTFKDLNVPPALISF
                     AVAAANVKDVITPEFKKPNSSVLLVKIKKNENNIPDFEDIKIKYGEIHEAIKKGVVLS
                     AHTVGYGGIAAAISKMSLGNELGFRTGFKIADDWFDASYGDIILEVENTSSLEDSIEI
                     GRTTVEKEISAGNICISLEEIKSAWLKPLNSIFPVKKDGAGRIESYGFNEEKTFKSSI
                     SIAKPRVFIPVFPGTNCEYDTKRAFDRAGAKAEIFVFKNLSSQDIKDSIKEMNSHIRQ
                     SQIVAFPGGFSAGDEPEGSGKFIAAVFRNELLKDAVTDLLKNRDGLIIGICNGFQALI
                     KLGLVPFGEITGMEEDYPTLTYNKIGRHISTFVRTKVVSNLSPWMRGCRLGDIHLLPV
                     SHGEGAFAANEKWIERFKTNGQIATQYVDESNAPTYDGFNNPNGSIEAIEGITSPDGR
                     VLGKMSHSERLQEDIYKNIPGNKDQRIFESGVKYFK"
     gene            23773..24261
                     /gene="purE"
                     /locus_tag="PKK75_01660"
     CDS             23773..24261
                     /gene="purE"
                     /locus_tag="PKK75_01660"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNZ82331.1"
                     /translation="MKVAIVMGSDSDFPVVEKGYNILKEFGVETAVRVISAHRTPFEA
                     LEFARNANKDGYELIIAAAGKAAHLPGVLAGCTTLPVIGIPVKSSELDGMDALLSIVQ
                     MPPGVPVATVGINSAENAALLAIQILSVKYEDLRHKFSAYKKQMAEKVFEKDKKLNEK
                     LM"
     gene            24271..25671
                     /gene="purF"
                     /locus_tag="PKK75_01665"
     CDS             24271..25671
                     /gene="purF"
                     /locus_tag="PKK75_01665"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNZ82332.1"
                     /translation="MYLDDYGLNEDKLHEECGVVGVYTDNPDIASQLIYYGLFALQHR
                     GQESAGIAINTGKKIEFHKDMGLVADVLNQEIIDRLIGNIAIGHVRYSTYGDSEKQNA
                     QPLVARYREGSIALAHNGNLVNAGSLRDMLENEGVIFQTSTDSEVVVNLVARHYRGSI
                     VKSIQTTMEVVKGAYAFAIMTENELIGVRDNYGLRPLCLGKREDGYLLASESCGIAAM
                     GGEFIRDIEPGEIVVINEDGVNSIKTTKYAQKKLCIFEHVYFARPDSNLDGVNVYQTR
                     YNSGRLLAQEAPVKADLVFSVPDSGTPAALGYAAELNIAYGLGIIKNRYAKRTFIEPK
                     EVLRQKGVKTKLSILKDIVQDKVVVMVDDSIVRGTTTKQIVKMVKEAGAREVHVRIAS
                     PPVTHSCFFGINTPDRSKLIGANKTTEEIKVHIEADSLHFLSLESLLKATGSTQGYCK
                     ACLNGDYPMEVPNNGI"
     gene            25680..>26621
                     /gene="purM"
                     /locus_tag="PKK75_01670"
     CDS             25680..>26621
                     /gene="purM"
                     /locus_tag="PKK75_01670"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNZ82333.1"
                     /translation="MDKLTYKDSGVDVNSGYETVRLIKDYVKKTHTKGVISNIGGFGG
                     MFEINKDEYEEPVLVSGTDGVGTKLMVAFMADKHDTIGEDAVAMCVNDVICQGAKPLF
                     FLDYIATGKLKPQKAASIVKGVAEGCVKAGCALIGGETAEMPGLYGEEEYDIAGFCVG
                     IVDKKKIIDGSKIIEGDVLIGIPSSGIHSNGYSLVRKVFFSHKNYDVNQYVEELGSTM
                     GEELLRPTRIYPKLILELISKFEIKGLSNITGGGFYENIPRMFPEGITADIDTENINV
                     LPIFKLMQKEGNLDIDEMYGTFNMGIGIVLALGKDILD"
     assembly_gap    26622..26721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26860..27486
                     /gene="purN"
                     /locus_tag="PKK75_01675"
     CDS             26860..27486
                     /gene="purN"
                     /locus_tag="PKK75_01675"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNZ82334.1"
                     /translation="MPQVKIGVLISGSGTNLQSLIDNTEKGNINGIITVVISNRKDAF
                     GLERARRHGIDAVYVRQKDYESFDKFNDAITDELKSRGVELVVLAGYLKILTRKFIEE
                     YKNRIINIHPSLIPAFCGGGYYGIKVHEEAINYGVKLSGATVHFVDEGADTGPIIIQE
                     AIEVSCEDTAETLQQKVLKIEHKILPLAVKYYCEGKIQISGRKVNIKE"
     gene            27491..29047
                     /gene="purH"
                     /locus_tag="PKK75_01680"
     CDS             27491..29047
                     /gene="purH"
                     /locus_tag="PKK75_01680"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNZ82335.1"
                     /translation="MRALISVSDKRGIVEFARELENLGIEIISTGGTQRALQEAGVKV
                     IGISEVTNFPECLDGRVKTLHPKIHAGILAMRKNPEHMKQLSELNIETIDLVVVNLYP
                     FKQTILKDGVTRQEAIENIDIGGPTMLRSAAKNYQDVAVLVDPEDYGIVLEELKENKG
                     VSLDTKFYLCSKVFAHTAHYDTLIASYMKKERKDSSLPDTYTMTYEKIQDMRYGENPH
                     QKAAFYKEVENAEGLLASAVQLHGKELSFNNINDTNGALELLKEFEEPCVVACKHANP
                     CGVGTADNIYDAYLKAFDADPVSIFGGIVAFNREIDEKTAEELNKIFVEIIAAPSYSR
                     EALSVLKAKKNIRLLQIENILQKQGEYTCDIKKVAGGILVQEIDSKLLPEDEELKTVT
                     GAKPAEQEMKDLLMAWKIVKHTKSNAIIIVKDGQSLGIGPGQVNRIWACKQAIEHAYE
                     FLGKDSTKGAALASDAFFPFPDCAEEAAKAGITSIIQPGGSLNDDKSIEVCNKHNIAM
                     VFTNMRHFKH"
     gene            29056..30309
                     /gene="purD"
                     /locus_tag="PKK75_01685"
     CDS             29056..30309
                     /gene="purD"
                     /locus_tag="PKK75_01685"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNZ82336.1"
                     /translation="MIILVVGGGAREHAICWKLKQSKQLSKLFCAPGNAGISQIAECV
                     DIKADELEKLTEFAVDNKVDLVVVGPEVPLVAGLVDMLNEKGIKAFGPVKAGAKFEGS
                     KSYSKDFMKKYSIPSARYEVYTDSKNAIGDLHKFNPPIVVKADGLAAGKGVLICETIK
                     DAEEAIKSIMDKKQFGDAGDTLVIEEFLRGTEASLLCFVDGKSIIPMESARDYKKAYD
                     EDKGLNTGGMGCFSPNTIFTEELNKYIRKYIIDRTLEGFLNEGIDFKGVLFIGLMIEN
                     NQAKILEYNTRFGDPETEVVLPRLKSDLIDIMSKCIDGNLSECDLLWNDEKCVTVVLA
                     SGGYPESYEKGKEITGLNDVDDDIVIFHGGTKLRDNKIVTDGGRVLAVTALGTTLDQA
                     RSKVYKNIPKIHYEKMEYRKDIAKV"
     gene            30522..31793
                     /locus_tag="PKK75_01690"
     CDS             30522..31793
                     /locus_tag="PKK75_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ82337.1"
                     /translation="MNIHVVQSGETLWQIAGYYNVSIEDIVNINRLNNPDILLPGHAL
                     LIPTNSITYTVQQGDTIWQIAQKFGVSVQQLLWENRIMNPDLIYPGYVLKIPQRPKTE
                     IDVNGFTYFLGPEAVPVVREAGPYLTYLSPFAYLINEDGTLVNIADEPAIAESYTQGA
                     VPMMSIVNFTVNVEGQNIANIVLNNPDIVNKLQDNIISIMREKGYQGLNIDFEYVLPE
                     DREAYNRFLESTVQRFHNEGFFVSTSLAPKLSAEQAGLLYEAHDYPAHGRLADFVVLM
                     TYEWGWRGGRPRAISPLNEIRKVLDYAVSVIPRDKILMGFQIYARDWKIPFEEGDEAA
                     TFSVQDAINLAYEHMAEIHYEYESQSPYFHYTDAEGIAHEVWFEDARSAQAKFDTVKE
                     YGLRGISYWGLGYEFPQNFPLLGSNFQIRKY"
     gene            complement(31872..33203)
                     /locus_tag="PKK75_01695"
     CDS             complement(31872..33203)
                     /locus_tag="PKK75_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNZ82338.1"
                     /translation="MDFIISVIIFTLSLIYSILKHIPTLVPLLIGMLSFSITAINRGY
                     GIRNVIKMVLRGMKKSLSLIPIFALIGMITALWRACGTIAFFVYYGINIMNPNYFILY
                     AFLLSCFVSFAIGTSFGTAGTIGIVLIVLAKSGGVDIYTAGGAIISGAFFGDRCSPVS
                     SSANLVAMVTGTDLYDNIKNMFITGALPFVAAVAVYFILSKYNPINVANNGLIDKLPV
                     NFNLHPITILPALVIFLLAALRKNVQFTMFSSILTAIFVCRYFQNFSFQQIFNYMIFG
                     YMNTDPELSGIISGGGLMSMVSVTFVVLIASSYSGIFEGTGMLNDIQGALVKISKKTG
                     VYITTVFTGVITCAFCCNQTLPILLTQDLMKNIYKKNGLTKSHLALDIENTVILIAEL
                     FPWSIAIAVPLATIDVGARAIPYAVFLYLVPLLAPLTPKFIKKTMNLSLSK"
     gene            33440..34639
                     /gene="megL"
                     /locus_tag="PKK75_01700"
     CDS             33440..34639
                     /gene="megL"
                     /locus_tag="PKK75_01700"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018826 - methionine gamma-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009087 - methionine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009093 - cysteine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HNZ82339.1"
                     /translation="MNKDKLSKMNFSTRAVHAGYKPNEYGALATPIYQTSTFIFDSAE
                     QGGRRFALEEEGYIYTRLGNPTTSQVEDKLASLENAEAAVAMASGMGAITSVIWSIVN
                     AGDHIIAAKTLYGCTYAFLNHGLAKFGVEVDFVDTSDPENVRKAMKPNTKMVYLESPA
                     NPNMLLTDISEVSKIAHEVEGCKVVVDNTYCTPYLQRPIDLGADVVVHSATKYLNGHG
                     DVIAGFAAGKKEFMDTVRLFGIKDMTGSVISPFDAYLINRGMKTLEVRMEKHCSNAQK
                     VAEFLESHPAIEWIGYPGLKSFPQYELGKKQMMLPGAMIAFEVKGGLEAGRKLMNTVE
                     LCSLAVSLGDTETLIQHPASMTHSPYTPEERAMSGISEGLVRLSIGLEHADDIIDDLK
                     QALDKLV"
     gene            34679..34867
                     /locus_tag="PKK75_01705"
     CDS             34679..34867
                     /locus_tag="PKK75_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNZ82340.1"
                     /translation="MRAKVDRDACISCGLCETICPGVFELDDESISTVKVDPIPEEYE
                     NCTKEAEEECPTDAIHAE"
     gene            35019..35924
                     /locus_tag="PKK75_01710"
     CDS             35019..35924
                     /locus_tag="PKK75_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNZ82341.1"
                     /translation="MKYLIGIDAGGTKSELIAYNLNDSPIFNLIGGFGNPSVNLEKTV
                     NNLISLIYDCVNELGREDCQLIAVGMAGVETGNYAELIKKYVEGAFVIKTIVLNDAEM
                     ACKAYFGDKDGILAISGTGSSCFVQKDGEGEMVGGWGHILGDEGSGYHTVMQVFKNII
                     HKYDRALPFDSLSKALIKEIGGSTRSDIMNFIYNNEKNKVAALFPIIVDYANKGDSYA
                     ILLLENAGRYLAELTQVAYKKKNFKGPAKIGLKGGVFHNSCFMLSEYMNVLQEGLDLY
                     DVIDEDISATKAVCNIYKASKKGAS"
BASE COUNT        12878 a         5719 c         7934 g         9519 t
ORIGIN      
        1 aaaatgtcag gtatctgttc cctgtgaaaa gggaatacca agagggtaat tctgagttat
       61 tgacatattt cataaataag ttataattag gctatattat tgaaatgagg tgttttgaat
      121 gcccaggccg agaaaatgga gaaaggtttg ctgcctgccg gatgtaaacc gttttggacc
      181 gctggatttt tctgtagaga aagaacattt tgttataatg tccgttgatg aatatgaaac
      241 aataagattg atagatctgg aaggtctttc ccaggaggaa tgtgcaggtc aaatgaatgt
      301 ggcaaggact actgtccagg gtatttatca ggaagcgaga aaaaagatag cagaatccct
      361 ggtaaacggg aaggttctta gaatagaagg cggagattac aaactttgtg acggggacgg
      421 agattctcac aaagaaaggt gctgccatag aataagatgc ggcaagctga ttagtgaata
      481 aatgttctga acctatgtca ttttagatag aatttaataa agattacata agctttagta
      541 aattttgtaa agatttataa tttgttattg caataggttt tttgttctgg tataatcgat
      601 tgaaaagtta ataagatact gtgattggaa gcagtaggtt tgtaaacaat gtaagagagc
      661 cgccggttgg tggaaggcgg tatttgaaaa actgaactcg tccatgagta tttgaagcga
      721 aataaagtag tttcaaacgg tgatgccgtt atagtattga gtgaggctta ggcctaattt
      781 gggtggtacc gcggaatctt cgtcccaaaa gggaggaaga tttttgtatg ccctaacccc
      841 atatgttcga gctttagcga tgaacagatg gctgataggt caacagcaac gagttcccaa
      901 aatatgcgac caaagggagc atatattttg gtgaaggtcg actgcagtat gccccaaagg
      961 gagcagatat tttggtgaag gtcgactgca gtatgccctg accctattaa acaagaataa
     1021 aaatggaggc aaaaaatgaa atcatataat ccacaggcta ttgaaaaaaa atggcaggaa
     1081 atatggagaa agaacgagac ttttaaggca aaggacaata cagaccagaa gaaattttac
     1141 ggtcttgtgg agttcccata tccatcagga gtaggactgc atgtaggaca catacgtgca
     1201 tacacttcta tagaggtaat ttccagaaaa agaagattgg aaggatataa tgtattgttt
     1261 cccattggct gggatgcctt tggtcttcct acggaaaact atgcaataca gacaggaaag
     1321 cacccaagaa aggtcacgga tgataatatc aggatattta ctgcacagct taatgcagcg
     1381 ggatattcat ttgactggtc aagaagcgtt gatacaacag atcctgagta ttataaatgg
     1441 actcagtgga tatttgtgca gttattcaag catggtctgg cttataagga taaaacatat
     1501 gtaaacttct gcaacagctg cagggttgtt cttgcaaacg aggattccca aaacggcata
     1561 tgcgaccgct gcggcagcca ggtggtgcag atggaaaagg atgtctggtt cttgagaatc
     1621 agagattatg cagataagct tttagacggt ttaaacgaag ttaattattt accccgtatc
     1681 agacttgaac aggaaaactg gataggccgt tcttatggtg ctgaagtcga ttttaaatta
     1741 aaggatacag aggacaattt aagagtattt accacaagac cggataccct ttacggggtt
     1801 acatttatgg taatggctcc agaacatcct ttccttaaaa agtacgagca caagatcaga
     1861 aatatggacc aaataaaaga ataccaggaa gaaacaaaaa agaaaaccga gtttgaaagg
     1921 gtccagctgg ctaaaagcaa gacgggtgta gagataaaag ggcttactgt aataaatccc
     1981 gtcaataaag aagaagtgcc ggtatgggtt gcagactatg ttatgatggg ctatggaaca
     2041 ggagctatta tgggtgttcc cggccatgat caaagagact gggattttgc aaaaaaatac
     2101 aatattccca taattgaagt aataggcgga ggtaatgtaa aagaggaagc atttaccgat
     2161 gtagaagacg gaatacttgt aaattcgggt atgattaacg ggttaagggt taaggatgca
     2221 atagcaaaga taactgatta tttggtagaa aataattttg gagaagaaaa agtaaattac
     2281 aagatgaagg attgggcttt caacagacaa agatactggg gcgagccgat tcccataatt
     2341 cactgtcctc actgcggcat ggtcaatgtg ccggaggatg agcttccttt gctgctgccg
     2401 gatgttgaaa attaccagcc caccgattca ggagaatcac ccctggcaaa tataccggaa
     2461 tgggtgaatg taaaatgccc tgtatgcggt gaagatgcaa aaagggaaac tgacaccatg
     2521 cctcaatggg cgggttcatc gtggtatttc ttaagatata ttgaccccca caacaaagat
     2581 gcaatagcat caagggaaaa gttcgactac tggggtccta tagactggta caacggcggt
     2641 atggagcatg ttacaagaca cttgatctat tcaaggttct ggcacagatt cctctatgat
     2701 ataggcgctg tgccctttaa agagccatat gcaaaacgta ctgcccaggg cttgatactt
     2761 ggtgcagacg gggagaaaat gtccaaatca agaggcaatg ttgttaatcc caacgatata
     2821 gtagacgaat acggtgcgga tactttaaga acttacatta tgttcatagg ggattatgaa
     2881 aaatatgcta tctggaatga tgcaagcgta aagggatgta agagatttct tgacaggacc
     2941 tggaaaatgc aggagatgaa aagttttgac aagggatatt ccgaaaaaca catgactctt
     3001 atgcataaaa caattaaaaa ggtcagcgaa gatttcgagg ctatgaaatt taacactgca
     3061 attgctgcca tgatgacctt tgtaaatgaa attttcaacg atggatacat tacaggcgac
     3121 gaattaaaaa catttattac tcttctgtat cccgtagctc cgcatattgc tgaagaaata
     3181 tggcaggaac agggttttga aggaatgctt aaccagacaa agtggccttc ttgggatgaa
     3241 gaaaaaacaa ttgaggatat cattgagctc cccgtacaga taagcgggaa ggttagaggg
     3301 aaaatagaat tacccaagga tgcggattta aataccgtaa gagaattaac acaagccgac
     3361 gaaaatattt taaaatattt agaaggaaaa acaattataa aagaaatata tattccgggt
     3421 aaaatattta atttagtagt taaatagcgg gaaaatgcgg atcttccaat aaataaggaa
     3481 gttccgcatt tttatttgcc aagatgatat agtaggaaag tataatagaa atcgtttaaa
     3541 cataatgtat aggggtttac ataatttaaa ttatgtaaac agtattatac aattaaaacc
     3601 acatagtaca gcagtataaa aggcaaactg tttttaatcc tggaattgga gaaaattgtt
     3661 tgtttttgta aaaagtatta aactcacatt ttataaagca actgaacgtt tattgcacag
     3721 ccttcatata atgtaaaaaa aaggggtgag ttcgtgtttg acataaataa atattacaat
     3781 acatatccaa atggcggatt tatggactac aatgcaatga tggcaagctg tttctgcaaa
     3841 aaatcatatg taagagttct taatacattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnc aatgatggca agctgtttct gcaaaaaatc atatgtaaga gttcttaata
     4021 catttaatat gccgctggat atccaggtca atgacattct tatgggagaa aatttacagt
     4081 caggggagtt tacgaggtat gtgaagtttc ctccgtcttc ctaccatgtt gtaatgcacg
     4141 caaagggcga tttagtattt gaatcggata tagacataga taacaacttg acatacactg
     4201 ccgttgtttc agcagatgac gaagatatat cagacatctg catattaatg gtacctgaaa
     4261 aaaaagtgca ccatatgggc agcaatatgt cagcactaag actggcaaat ttatgccctg
     4321 atgaaccgga ggtagtattg tctgcatctg acggaacggt tctgttttct gatattaagt
     4381 acggaaaggt atcctgcaat gtggctgttc cttccggaag atatatattg catttaagga
     4441 aaccggacgg tacagaaatt ttggagcaca gtatggatgc tgcaccttat atgcattaca
     4501 cattgtttgc agcaggcagc caaaatgata atagtgttaa aatcattgta cctgaagatg
     4561 gggtaaacta cctggatttg tgctgataaa actagtgagt cccaataata ttattgttac
     4621 aatgcaataa ttatgatata attcatcaat aaaagatgaa gaggtgggct atgaaaacaa
     4681 tagggataat cggcggcatg ggacctcttg ccactgtgca tttattcgag agaatagttt
     4741 taagaacaaa ggcggaaaaa gaccaggatc acatacgtgt gttaattgac agcaatacca
     4801 atattcagga caggacaaag gcaatactcg gcatcgggga aagtcctgta aaagaactgg
     4861 ttaattcagc gaaaatcctt gaaaattccg gtgcagactt tttaataatg ccatgcaata
     4921 cggcacacta ttttattgat gaaataaaga aaaatattaa tattccattt attaacatgc
     4981 ctgatgagac ggtaaagtat acatatgaca aatatggaaa agatgctctt gtaggaataa
     5041 tggcaaccga tggaacaatt aaaacaaaaa tttatgaaaa ttattatgcc gggctgggaa
     5101 taaaaaccct ggtcccgata aaaactcagg gcaaaattat ggaatttatc tatgatgtta
     5161 tcaagaaggg aaactatgat gcaggaacgg accttttctt tgaatgtgta aatgaattga
     5221 aagacctggg agcaactgca tttttactgg gatgcacaga gctttcatcg gcacagtatc
     5281 tatataaatt tgaaggaagc tttattaatc ctatggaggt tttggcagaa agggctataa
     5341 tttttgccgg cggacagtta aactgataat gctgcttaca ccctcacagg tttgaaatct
     5401 tgaatgaggc gaaagatttc attgtccttc acattattca ggatgaaagg tctgatgctc
     5461 ttattattta atatttgtac agtatgttat tattcatggg gacacattcg tccccaaatt
     5521 tcacttgaat tatacgaaca tttataatat aataatataa aaacattcgt cggagataat
     5581 atgaaaaaat ataaaagaaa tcaaagaatt ggtgcattaa tgaaaatttt tgccgaaaaa
     5641 ccaaattact tattttcata taattacttt tgtgaaattt tcaatgcagc aaaatccaca
     5701 ataagcgaag atgtggcaat tgttaaggat ttagcggaag agatgaattt cggaaggatt
     5761 gaaactattt ccggagcttc ggggggagtt ttgttcatac caagattggg aaataaggaa
     5821 agaaatgata ttcttgaaca cctgtgttcc ttattttcgc aaaaaaaccg gatacttccc
     5881 ggcggattta tttatatgac agacatattc aacaatccag ccatagtgtc gaatattgct
     5941 aaagtaattt cttctgaatt taataacagg catatcgatt atatcgttac cgtagagaca
     6001 aaaggaatac ccgtggccat aatcacggga caaaggctga atgtgccagt ggctgtaata
     6061 agaagaaaca ataaaattac agaaggagca acggtcagca ttaattatgt gtccggctca
     6121 tctaacaata tacagaccat gtcattgtca aaaagatcat taaaggaata ttccaagatt
     6181 cttatagtgg atgactttat gaagggcggc ggaacctgca agggaattga ggacatgatg
     6241 agagaattca atgcagatgt ggtgggaact gctgttgtta ttgaaactat ggagcctgaa
     6301 aagaaattgg tagacaatta tacttcatta ttggtgctta ataatatgag tttaaaggaa
     6361 ggaattattg aaattcatcc aaatactaaa ttgttaaaat aaagtcatat gtttaagaac
     6421 aaaattatga aaatgttttt ttgcattctt cagtttttta tagtacaata gataaaatgg
     6481 acataatttt gggggaaggt ggtgacaaca tgaagatatc agatgtgcgg gtaaggaaaa
     6541 tcaattctga gggcaagatg aaggcaatcg tttccgtaac ctttgatgat tgttttgtgg
     6601 tacacgatat taagataatt gaaggtcagg aagggttgtt tatagcaatg cccagcagga
     6661 agatgccgga tggggaattt aaggatatag ctcatcccat taattctgaa acaagaaatc
     6721 ttgtatcaga ggcagtattc agagcatatg aagaaaagcg cacagaagaa agcgagaacg
     6781 tggaagaagc tgctcttttg taacgctgtt gctgatggtt ttcaatcaac agtactcggg
     6841 tctttcaaag acccttttat tgtgcaaaca acaaggttcc cgggtagcca agcaatcttc
     6901 gattgcatcg ttgggtgggg ttcttattat gcctaacccc atttttcaga gtcattttgc
     6961 tgaaaaatgg ctggtaggtc aaacgcacag aattctcaat ttatgtgagc ttaagcgaac
     7021 tgataaattg aagaattccc ctgcaatata tgaatatttt tttgacttag caaataatat
     7081 gatatataat aatttggaat tacagtttcc attaaggaga attaattatg aatatgtcaa
     7141 ttattttggc agcaggtgaa gggaccagaa tgaaatcaga tattcctaaa acactgcaca
     7201 aagtttgcgg aaaagaaatg attaaatata taattgaagc atcagtaaga tcaggcatag
     7261 aaaaaaatat tgttgttttg ggacatggaa aagaaaaagt tggacataat atcaaagaaa
     7321 tgaatgtaca aactgtcgag cagcctatcg gcgaaagctt cccctatgga acgggttatg
     7381 ccgtaatggc tgcagaaaac catattgacg atgtggacac ggttttaata ctttgtggtg
     7441 acggaccact tatcagaaaa gaaacattga ccggcttcat gaactatcat tttgaaaatg
     7501 attatgctgc aactgtaatg acagcaaaat tagataatcc caaaggttac ggaaggatag
     7561 taagaaacag ggatggttct attgacaaaa ttgttgaaga aaaagatgca tctgaagaaa
     7621 ttaaaaatat taaggaaata aacacaggta tattttgttt taaagggcac ctcttaaagg
     7681 atgctttaaa aaaaataacc agggataatt caaagaatga atattatcta accgatacaa
     7741 taaaagtatt aaataaccag ggtactaaaa caggtggatg gcttctagag gatgcagatg
     7801 aaatcaaggc tgtaaatgac agggttcagc ttgcggaagt aaataaaata atgcagcgaa
     7861 gaatcaacca aagacacatg tattcagggg ttacaattgt tgacccagat aatgcatata
     7921 ttgatgatac agtgataatc ggcagagata cggttattta tccaaataca tatttggagg
     7981 aaggaacagt tataggggac aactgtaaaa taggacctaa ttccaggata atcaattctg
     8041 tcttatgcaa taatgttact gtggaaaatt ctaaaatgat tgaatcctat gcagacgatg
     8101 gaacattaat aggccccttt gctttcttaa gaccgggaag caggctgggg aagaatgtaa
     8161 aaataggaga ttttgtagaa gtaaaaaact ctatcatcgg ggataattca aaatcatccc
     8221 atctgtcata tattggcgat gcagaagtag gcaaacatgc taatattgga tgcggagttg
     8281 tatttgttaa ttatgacggt aagaataagt ataaaacaat agttggggac ggggcttttg
     8341 tcgggagcaa ttccaattta gttgctcctg ttacaataga agagggcgga tatgtggctg
     8401 caggttcaac gattactgaa aatgttaaaa aaggcgattt ggcaatcgca agagcaagac
     8461 aggtcaataa agaaggaaaa ggcagcaaca ggtattaaag aggttaatat gtttataatt
     8521 gcaggactcg gcaatccagg caaggaatat accggttcaa ggcacaattc aggctacatg
     8581 acagttgaac atttggcaca aagactaaat gtaaaattaa ataagctgaa atttaactcc
     8641 gtatatgggg aaacatacat agacggagaa aaagtcatgc ttgtaaagcc ggttacctat
     8701 atgaacagaa gcgggattgc cataggggaa attgttaagt tttacaagct acccacagaa
     8761 aacatcatag tcatatatga tgatattgat atacccctgg gtactttaag aatcaggcca
     8821 aatggaagtc caggaacaca taacgggatg aaatcagtta ttgaaagcat aggaagcagt
     8881 tttccccgca ttagaattgg aataggaaga aatgaagata tggaccttgc cgattatgtt
     8941 cttcagagtt tttctcaaga tgaaaaagct gtagtcagca acattattga aaaggcagct
     9001 gaagctgctc ttgaaattat tgaaaacagc gtggaaagcg caatgcagaa atataatgtc
     9061 aaaggtttaa gtgaatcata gcaaggaagg gggacgccat gtccccagat taaatatatg
     9121 aacaaagtct tatttgacca gtttcaaaga acagaaaaat ttaaaaagat atgtgataat
     9181 tataagagtg gaaaaagtca gctgctccaa gggctaaatg aagaaggagc agcttatttg
     9241 gcgtgcaatc tgttagacat atccgataaa gtattaataa taacaagcag cgaagcaaaa
     9301 tcaaagaaat atgaagaata tattaaagct tataccgatc attcaaaccg actccagccc
     9361 aaagaattta ttttgtataa tgttgacgcc ttaagcaagg atgtagacca caagaggtca
     9421 gacttgcttc atgatatttt aaataggaag aaaaacattg taacagcttc aattaattct
     9481 ataattacaa gagtgatgtc taaagataga tttaaggaaa gcattataga attaaaatac
     9541 ggcttaagct atgacatgaa tgaactaaga agcaggctga ttaagctaaa atacgagaga
     9601 gtggatgcaa ttgaaggagt gggacaattt tctgttagag gcggaattat tgatattttt
     9661 tctccttctc aagcaaatcc atgcagagtt gagttttttg atgatgaagt agactccata
     9721 agactgattg atttaaaaac ccagagatct gttaaaaacg tagggtccgt taagattctt
     9781 ccctgcagtg atgttttatt caaaaaagaa gaaataaaaa caatattagc tgaagttgaa
     9841 aaagattata atcttagaca aaacaaaaat cttggcaggg aagcggcaaa aaacttaaaa
     9901 aacctttaca actcttatca agacaagctg atgtccggta tgggaattga aaatgcagac
     9961 cttttggtag cttttacaaa ggagtccttt tcaagcattt tagattattt tggggatgat
    10021 gagcttattc tgcttgttga tgaacctgaa agaatatttg aagagctcag aactattaac
    10081 gaaagcttcc ggcttaaata cagcgaatta ttcgaaaagg gcgaaatttt ttcaaatcag
    10141 agccaggtgt ttataagcga agatgaactc cttgaggaaa ttttaaaaag acccttcatt
    10201 tccttaaacg gaaaagagaa agcatttaag acggatactt ccgtccacat gctgttcaag
    10261 gaagctccct cttattacgg caggatcgaa gagctgtcaa aggacctcaa caggctgaaa
    10321 tataagggct acaaggtaaa tatcgtactt gccggctatg aaagctgcaa gaagctttat
    10381 aatctattaa atgattatga atgctctgcc atattgtcaa gggatgcaaa tacggctgaa
    10441 agcggccaag tggtaataac accgggcgaa ctgacaaagg gatttgaata ttacgataac
    10501 aagatccttg tacttacaga aaatgaagta cttggctcag ccagacgcaa atcccgtaag
    10561 gcagtaaagc gaaaaggtgc ggcaatagat gttttcaccg atttaaaggc aggagactat
    10621 gtagtacatg aacatcacgg catcggtcaa tacgttggaa ttgaaaaaat caaggtgcag
    10681 aatatacaga aagactatct gtgcatcagg tacaaggccg aagacaggct gtatgttcct
    10741 gttgaccaaa tgtctctcat tcaaaagtat atcgggtctg attctgaaaa accgaagctc
    10801 aataaaatgg gaagctcaga ctgggcaaaa accaaggaac ggtcaaaagc tgcaatagaa
    10861 aatatggcag ccgacctgat taagctgtat gctgaaagaa aggtttcaaa aggatttgca
    10921 ttttccggcg acactgaatg gcagaaagaa tttgaatata aatttccctt tgaagaaaca
    10981 gaagaccagc ttcgctgcgt aaaggaaata aaaaaggaca tggaaaaacc catttgcatg
    11041 gacagacttc tttgcggcga tgtgggcttc ggaaaaacag aggtggcaat gagggctgca
    11101 tttaaagcgg ttactgactc aaaacaggtg gcaatgctgg ttccaacaac tattctggct
    11161 cagcagcact ataccaattt agttgaaaga ttcagaggct atcccattag aattgaaatg
    11221 ctcagccgct ttagaacgca ctatcagcaa aacaaaatta taaatgagct taataaaggt
    11281 ttggctgata ttgtaatcgg tacacataaa cttctttcca aggaccttaa attcaaggat
    11341 ttgggtcttt tgataataga tgaggagcaa agattcggcg taagacataa ggaacttata
    11401 aaacagctca aaagcaacat tgatgttctt acactgtcgg ctacacccat accaaggacc
    11461 cttcatatgt caatgatagg tgtaagggac atgagcatta tatcagagcc cccgggagac
    11521 aggcttccca ttcaaaccta tgttattgaa tacaatgaag gattgataaa ggatgcaatt
    11581 gaaaaggaaa tcttaagaaa gggccaggtc tattatgttc ataacagagt ggccgacatt
    11641 gaccgtgcag cagccaaact gagaagaatt ctgcctgatg cacgcattgc ggtagcccac
    11701 ggccaaatga gcgaacggca ccttgaaaat gtaatgtttg agtttgtcaa tagagaatat
    11761 gacattttgg tatgtacaac aataattgaa accggaatgg acatacccaa tgtaaacaca
    11821 ctgattattg acaatgccga ttatttggga ctatcacagc tatatcaatt aagaggaaga
    11881 ataggcaggt caaataaagt ttcttatgct tatctcacct atgaaaaaga caaaatgctc
    11941 tcagaagttg cagataaaag gctcaaggca ataaaggaat tcacggagtt cggttcagga
    12001 tttaaaattg ccatgcgtga tctggaaata agaggcagcg gcaatatact gggtcaggaa
    12061 cagcacggtc acatgctggc aataggatat gatttgtatg ttaagttctt ggagagagca
    12121 gtaagggaaa tacaaggcaa acaattggaa gaggatattg aaacgtctgt ggatttaaat
    12181 gttgacggct acattccatc atcatttatt gaaaatgaag agcaaaaaat agagatatac
    12241 aaaaaaattg cagcggcaga aaataaaaat gatatacttg atataactga agaaattata
    12301 gacaggttcg ggaatattcc gcctcaggtt ggcaatctct tgaaaatatc ttatattaaa
    12361 tcgctatgca aaaggcttca cataaaagca ataagccagg caggaaatac agtaagtatt
    12421 gaaatgaccg gaacaagtga cctgggacaa aatataatag aattccttat taaaaattat
    12481 tacaaaaaaa taagctttgt tggaacaaac gagcctattg ttaaatatag attagattct
    12541 cttgaacaag aaaaattact caaggaattg gaaggatttt ttgaaatact aaataatttt
    12601 aaagcagata cagctgctgt ggaggaaaaa catgaacaaa tttaaaaaat cattattctt
    12661 aataacttta atgtgcatag ctttagtatt tacagcatgt tcagacaaag aaagccagga
    12721 tgccgttgaa ggtgttatgg caacagttaa cggcaaggca atcatgcaaa aagactatga
    12781 tgatgcaata gcatactaca agtcttatgt tgagtatcaa tttggtgaag atgcctggga
    12841 aaaagaggca gcaagaggta tgacatataa agaaatatat gaggaatacg taatgaatga
    12901 aatgactaat tcattgcttt tgctggatgc agccgaaaaa gaaaaaatag aagtaacgga
    12961 agaagacaaa caacacgcat tggaaaattt taaaataaat tttgaaaacg acgaagatta
    13021 taaaagcttt ttggaacaaa gaaatatgac tgaagaattt gttttaggag agctgactaa
    13081 agaaatattg gttaataatt atataacaac gaaaattgac agcttgctgc ctggtgagga
    13141 tgaattgaaa actctttttg atgaaatgaa aatgaatact caggtaaaag caagccatat
    13201 tcttgttaat acagaagaag aagccataaa agctatcgaa agaataaata agggcgaaga
    13261 tttcgcagaa attgccagag aattgtccat tgatcccgga agcggaccaa acggagggga
    13321 tttagactac tttaactatg cacaaatggt aaaacctttt tcagaagcag catttgcatt
    13381 ggaaataggg gaagtgagca agccagttaa gtctgattac ggatatcata taattaagct
    13441 gactgacaaa atagttgatg aagatataac agtggaatcg gaaaaagaca agcttattga
    13501 gtattacaaa acaaacagat atcaggactt attagaagaa ctcaagagca atgcagtaat
    13561 agtaaataat taaaaatttg ttaattttag taaaaaaatg ttgataagtt taaattcttc
    13621 cattataatg aataatatat ttctattctt tgataatatt tacaacatta tggcaaacta
    13681 gtaaggataa aattcgctaa agtgtaaata atagaaaata ttaaaagaaa tgtcataaaa
    13741 atatatgtaa ttggaggaat gtgagcaaat gaaagcaaca ggaattgtca ggagaattga
    13801 cgatttaggc agagtagtaa ttcctaaaga aattagaagg accttaagga taagagaagg
    13861 ggacccttta gaaatattta ccgatagaga aggtgaaatc atattaaaga aatactcgcc
    13921 tattggagaa ttgactgaat ttgcaggaga atatgttgaa tcattatttg aaactaccag
    13981 gcatattgca ttaattacag atagagacgg tgtaattgct gtttccggaa gttctaagaa
    14041 agattataca gaaaaaagac tgagccctga actggaaaaa atcatcgaga gcagagagat
    14101 ttatcaaaca gatgcaactt caaagccaat aagaattaca gccaatgaat tcaatcctga
    14161 caattataca agtcaggtta tttcacccat tatggtacat ggagatccaa tcggagccgt
    14221 tatactcctg tccaaagaca aaggctccaa gatgtctgaa gttgaagaaa aattaattaa
    14281 gacggcaagc atatttttat caagacaaat ggagaattaa agatgcccta accccatatg
    14341 ttcgagctat ggcgaagaac agatggccgg taggtcattc acaacgagcg caaattttgt
    14401 gtgacgacag gaacaaacaa aattgcgcag cgagactgag agatgaccta agcagaggtg
    14461 ctttttataa attgaaggca ccttttattt tattaattat ggctaatgtt tggtacaagc
    14521 cattgacata tttgattttt tttgtcatac ttattatgtt gttaaaatat tttttaagca
    14581 tccttagttc taatccgaaa gaggtataat atgaaaatca ggtttttagg tgcagtaaat
    14641 ggtgttaccg gctcaaacca cttaatacag gcaagtggca ggaatataat gcttgattgc
    14701 ggtatgtatc agggtaagga tgaggattta aatgcagaag aatttgcagt aaatccggca
    14761 gaaatcgatt gccttttatt gagccacagc cacatcgacc acagcggaag aattccgttg
    14821 cttataaaaa aaggttttaa aggaaatatt tattgttcaa aacccgcata tgacctttgt
    14881 gaaataatgc tgttagattc agctcacatt caagagacag aagcagaatg ggagaacaat
    14941 aaagcaaaac gttcagggaa ggaatttgta gaacctctat acacacagga ggacgcttta
    15001 aaaagccttc agtatttcaa gccggtttta tatgatcaaa taattaatat agacgaaaat
    15061 atcactgtca gattcaatga tgccggccat atactagggt cttctatcat tgaaatatgg
    15121 ataaaagatg aggaagatac cattaaactg gtgtattccg gagacttggg catgcatgaa
    15181 aaacctttgt taaatgatcc tgttcagata aacaaggcag attatttaat aatggaagca
    15241 acctacggca acagaatcca cgaaaatata aatgaaagaa caggagagct tattaatatt
    15301 atattaaaaa cagcaaggag aggaggcagc gtaataatac cttcctttgc ggttggcaga
    15361 actcaggagt tgatttacga acttaacaaa tactatgaca gccatttgaa tgaaatcggt
    15421 actgaggaaa atgaactgag aaagatacct gtctatatag acagccctct ggcagttaaa
    15481 gcaacagaag tatttaaaag aaacagctat gccttcgaca aagaggcaaa agaacatata
    15541 atgaaagggg acaacccttt ggattttcaa aatctgcatt ttgcagtaac agcagatgaa
    15601 tccaaacagt taaacttttc caaagaacca aaaattatta tttctgccag cggtatgtgt
    15661 gaggcaggaa gaatcagaca ccacttgaaa cacaatctct ggagaaagga agccagtatt
    15721 gtatttgtag gttatcaggc agaaggtacc ttgggcagaa ggctcctgga cggagaaaaa
    15781 aatgttaaaa tattagggga agaaataaaa gttaatgctg aaatatacaa tattgaggga
    15841 ttttcaggac atgcggacaa acccgctctt ttaaactgga tagacggttt taaagaaaag
    15901 ccggggaaaa ttttcatagt tcatggagaa aaagaagcaa aagaagagtt agccatggaa
    15961 gtgaaagaaa ggtttaacat aaacagcata attccagagt ataatatttt atatgatatc
    16021 aaagagaaaa actttgtaaa tgaaattgtt ctggaatcta aaaaacaaaa aatagaggat
    16081 aagcatatca atgagctgtt aaaggaattt gaagaactta agaaagtatt tgaaaatgct
    16141 ctaattaaca ctaagagtta tttgaatgat gaagatattg acattgacaa aataaaaaat
    16201 atcaacaata atataaaaag tcttgaaaat gaaattatga atttaaccat aatcagtgca
    16261 aaataaaaga tgggaaaaaa ggaggaaaat atgaaggtat taatgacagg caatgaagca
    16321 attgctcagg gagcttatga agcaggagtc catttcgctg cggcctatcc aggtacaccg
    16381 agtacagaaa tattggaaaa tatagctcca cacaaagaaa taatatcaga atgggcacct
    16441 aatgaaaagg ttgccgtaga atcggcaata ggagcaagct ttgcaggagc aagagcttta
    16501 acagcaatga aaagcgttgg acttaatgtt gcagcggacc cgctttttac agtgggatac
    16561 acaggtgtta acggcggatt ggtactggta aatgcagatg atcccgggct tcattcgtcc
    16621 cagaatgaac aggataacag gtactatgcg agaatggcta aggtagccat gcttgaaccg
    16681 agcaactccc aggactgcaa ggacatggta atgaaagcca tggatataag cgaaaaatat
    16741 gacgtcctgg ttatgataag aatgactaca agagttagtc atagcaagtc aattgttgaa
    16801 acaggcgaaa gagttgaagt gccagtaaaa gcatacgtga aaaatccaca aaaaaacatt
    16861 atggcaccgg ccaatgcaaa agccgctcac ccggtagttg agaaaaaact taaggaactt
    16921 gaaaagtttt caaatgaaac cgacttaaac tacattgaat ggaataacaa aaaagtcgga
    16981 gttatttctg caggtgctgc ttatcaatat gcaaaagaag tatttggcaa ggatgcatca
    17041 tatttgaaat taggttttac tttcccgctt ccaatggaaa aaataaagaa atttgcagag
    17101 gaagttaaaa cactatatgt aattgaagaa ttagagcctt atatggaaga acaaatcaag
    17161 gcggcaggta tctcctgcat aggcaaagaa aaaataacca atatctggga gcttaatccg
    17221 gatattatta agaaatcaat tttcggtaga gaaaatgaag ttattgaata tgacaaaaca
    17281 atagcagtgg gaagacctcc cacactatgt gcaggctgtc ctcacagagc tttctactac
    17341 caattgtcaa agaaaaagaa taccgtgata acaggagata taggctgcta tacattgggc
    17401 gggtcacctc cgttaaattc aattgataca tgcgtatgca tgggagccag cataagcatg
    17461 gcacacggtg ccaaaaagat atttgaaaag aacaatgata gcaaacgggt agtagcaacc
    17521 ataggagatt caacattttt ccattcaggc atgacaagcc ttttggatgt ggtctttaac
    17581 caaagcaata ccataacatg catactggat aacagaataa cagcaatgac aggccaccag
    17641 gaaaaccccg gaaccggcta tacacttcag ggagaaatta ccgacacagt tcaaattgag
    17701 cctgtagtaa gagcacttgg aattaaaaat gtaaaaactg taaatcccat agaccaggct
    17761 gcaattaagg atgctctaaa ttgggccttt gatttggacg aaccatctgt aattatttca
    17821 agatatccat gtgctctcaa gaagtattct aaagaggata tcgaagaatt cggaccaatt
    17881 tcaggttact gccaggttga cgaggacaca tgcataggat gcaaaatctg cgtaggtaca
    17941 ggctgtccgg ctatagtatt taaaaaagaa ataaagaaat cttcaatcaa taaagtgcaa
    18001 tgtgtaggat gcgaactttg tctgcagtta tgtcctaaaa aggcaattaa ggtaggtgaa
    18061 taaaatgaag gatactaaaa atattttatt ggtgggtgtt ggcggccaag gaacaatatt
    18121 ggtaagtaaa atattatcca ctggccttat ggaagcaggt tatgatgtaa aaatgtcaga
    18181 aattcatggt atggcacaaa gaggcggaag cgtttcaact caagtaagat atggaagtga
    18241 agtcgcttct ccaattatag gaatcggcga agcagatatt ctggtttcct ttgaaactat
    18301 ggaagcactt cgctggcttg aatttttaaa gccggatggt gttgtagtaa tcaatgattt
    18361 ccaaattgct tcggcgccca tattgatggg aaaagctgat tatcctgaag gattgattga
    18421 gctgattaaa ggaaaagcaa aaacaaaggt cataaacgcc ggagaaatag ctgaagaatt
    18481 agggaatcaa aaagtaatga acgttgtttt gctgggtgcc ctgattaagt caatgcaatt
    18541 gacagacatt gactgggaag ctgtgataag gaaccaggta aaagaaaaat ttgtagatat
    18601 aaacattaaa gcgttcaata aaggaatgga agctgtttaa aaggaaggca tgctatgagc
    18661 tataagatat ttgttgttaa tacaggttca acttctacta aaatagcatt atatgaagat
    18721 gataaagaag tatttgtaaa aagtttaagt catccagatg aattaatcag taaatatgat
    18781 gatgttcaag accagcttca tatgagattg gaatttgtca gatcatattt ggaagaaaag
    18841 aaattcgatg taaaagactt atcttgtgta gttgcaagag gaggaatgct tcttccggta
    18901 aaatcaggcg catatgctgt taatgagctc atgcttgacc gagttgaaaa ccgtcctgtt
    18961 atggagcatg cgtcaaatct tgctgccccc attgcatatg atattgcaag agaagcagga
    19021 gtgccttcat atatttatga ttctgtaatg acagatgagt ttcctgacat tgcccgtata
    19081 tcaggcatgc ctgacatacc tagaaacagc acgagccatg ttttaaatac aagggctatg
    19141 gcaataaaat atgctaaaag catcgggaaa aaatatgaag atctaaatat tattgttgcc
    19201 catttaggcg gaggcatatc aattaatatt catcacaagg gtcagatagt agacttgctt
    19261 tctgatgatg aaggaccttt ttcacctgaa agagcaggaa gagttccctg cagagacttg
    19321 ataaaccttt gttattcagg taaatttgac aaaaaaacaa tgcagcggaa gctgagagga
    19381 aacggcggtc tgaaggcata tttaaatacc attgatgcaa gacaagtaga gacaatgatt
    19441 caagaaggca atgaatatgc aaagctgatt tacgaagcga tggcatatca aattgcaaag
    19501 ggaatagggg atctggctac agtggtagaa ggaaaagtag atgctataat tctcacagga
    19561 ggcattgcat attctaagat gcttacctcc tggataaaaa acagagtttc ttttatatct
    19621 catgttgaaa taatgcccgg tgaaaatgaa atggaagctt tggcttacgg tgccttgagg
    19681 gtgatgaggg gcgaagagaa agcaagagag tatacagaat aaataaccat tctgaacctt
    19741 atttaataat gtcgacagca gccaaaacgg ctgctgtttt tgtcatgccc tgacaatgtc
    19801 tatgactgcc tataccctta ctcataaccg gtaaaaacat ttagctaaat atgatgtatt
    19861 tgtcggtata tttttaaatg ttcacaataa tacaaatgaa tgacagtaca gatatgcgga
    19921 agttcgtagt ataacgaata aagaattaat ttaatattga gaacaatata gaaaccgttg
    19981 acaagggttt ctatatttgt tattattatt ttgaaaaatg ataacaaagg atggttatta
    20041 tgaactttga tgttaagagg atttttgttg aaaaaagacc gggttttaac actgaatcat
    20101 taaatttatt aaacagtttg aaggaaggct tgaatattga taaattaagc agcattagaa
    20161 tactaaacag gtatgatgta tcaaacattg accgggagga ttttgaaaat atagtaaaaa
    20221 ctgtattctc tgaaccaaac caggataccg tgtattatga agatgccggc ctaaaaggca
    20281 attattttgc agtggaatac ctgccgggac aatacgacca aagggctgat tttgcagccc
    20341 agtgcgcaca aatcgttaca ggaaaagaaa aaccacttgt taaatatgcc cgggttttcg
    20401 ttcttgaagg cgggctgtct gataaggatg ttgaaaaaat caagaattac tgcattaatc
    20461 ctgttgattc cagagaagca gaaatagcta agcctattag cttaagccat gagcagccat
    20521 cttcaaaaga tgttgaaaca gtagagatta taaataaatc ccctaaagaa ataaaaaagt
    20581 ataaaaatga cgcaggactt gccatgagct ttgaggattt attattttgc cgggaatatt
    20641 ttaaaaacac agaaaagaga aaccctacca tcacagaaat caaagtgata gatacttatt
    20701 ggtctgatca ctgcagacac acaacatttt caacggttat agaaaatatt gaatttgaaa
    20761 aaggaagaat aacagaggaa ataaaaagca catatgatga atatctgaat acacagaaat
    20821 ttgtctatga aagcaagaga gatgttactt taatggatct ggccaccata ggagccaagg
    20881 agttaagaaa aaaagggctg ttaaatgacc tggaaatatc cgaagaaatt aatgcatgca
    20941 gcattataat aaatgtggat atagacggga aaaatgaaga atggctttta atgtttaaaa
    21001 atgaaactca taaccacccc actgaaatcg aacctttcgg cggagcagcc acatgtctcg
    21061 ggggagccat aagggaccct ctgtctggac ggtcttatgt ctaccaggca atgcgtatta
    21121 caggatgtgc ggatccaagg caaaaaattg aagatacact tcaaggaaag cttcctcaaa
    21181 ggaaaattac aacagaagca gctgcaggat acagttctta cggaaatcaa atcgggcttg
    21241 ccacaggtta cgtaaatgag gtataccacc ctgattacac tgcaaaaaga atggagctgg
    21301 gcgcagttat tgcagcagca cccagggaga atgttaaaag agaaagtcct tctatgggtg
    21361 atcttgtaat acttttaggc ggaagaacag gaagagacgg cataggtgga gcaaccggtt
    21421 cttctgttga acacacggaa gaatcaatag ttttaagcgg ctcggaagtg caaaaaggaa
    21481 atgctcccga ggaaagaaaa attcaaagat tgttcaggaa aaaagaagta agcacactta
    21541 taaaaaaatg caacgatttt ggcgcaggcg gcgtatcggt tgccataggc gaattagccg
    21601 acggactaaa tataaacctg gacctggtac ctaaaaaata tgaggggctg gacggaacag
    21661 aactggctat ttccgaatcc caggaaagaa tggcagttgt tgttagaaaa gaagacgcag
    21721 agaaattcaa ccactatgcc cacgaagaaa acctccagtc aactgttgtg gcagaggtaa
    21781 ccgataacag aaggcttaaa atgacatgga gggggaagac aatacttgat ataagcagag
    21841 actttttaga tacaaacggt gtcaggcaaa aaacaagtgc aaaaatttca gaacctctaa
    21901 atataaaata tgaaagaaaa cttaacaata ataatataaa tgaactttgg cttgaaaatt
    21961 taaaagactt gaatgttgca agccaaaaag gactggttga aagattcgac tcaacagtag
    22021 gagcgggatc tgtaatagta cctatgggag gaaggacaca gcttacaccg gcagaaggaa
    22081 tgattgcaaa aattcctgtt gaagaaggag taacatccac ctgctctctc atggctcatg
    22141 gattcgaccc ttatctttcc aaggaaagcc cttatcatgg cgctatttat gcagtaatac
    22201 attctgttgc caaaattgtt gctatgggag gagattacaa taatatccgg cttacattcc
    22261 aggaatattt tgaaaagctt aacaaggagc ctgaaagatg gggaaaacct ctgagtgctt
    22321 tgttaggtgc ctacaaggta cagcatgaga tggaaattcc ctccattgga ggaaaggaca
    22381 gcatgtcagg tacatttaag gaccttaatg tgccacccgc cttaataagt tttgcagttg
    22441 cagcagcgaa tgtaaaagat gttattactc ctgagttcaa aaaaccaaac agcagtgttt
    22501 tattggtaaa gattaaaaag aatgaaaata atattccgga ttttgaagat attaaaataa
    22561 agtatggaga aatccatgaa gcaataaaaa aaggagtagt tttaagcgct cacactgtag
    22621 gatacggagg aatcgccgct gccataagca agatgagcct tggaaatgag cttggattca
    22681 gaactggttt taaaattgcc gatgattggt ttgatgcaag ctacggtgat attattttag
    22741 aggtagaaaa tacttcttca ttggaagaca gcattgaaat aggaagaaca accgttgaaa
    22801 aagaaatttc agccggtaac atttgtataa gtctggaaga gatcaagtct gcctggctga
    22861 aacccttaaa cagtattttt cctgttaaaa aggacggtgc aggaaggatt gaaagctacg
    22921 gcttcaatga agaaaaaact tttaaatctt ccatatctat tgcgaaacca agagtattta
    22981 tcccggtttt tccgggaacc aactgtgaat acgacactaa aagagccttt gatagagcag
    23041 gggcaaaagc agaaatattt gtgtttaaaa acttaagttc acaggatatc aaggattcta
    23101 taaaagaaat gaattcacat ataaggcagt ctcagattgt tgcatttccg ggaggattca
    23161 gtgcgggaga cgagcctgaa ggttcaggga agtttattgc tgcagtattc agaaatgaac
    23221 tgctgaagga tgcagtcaca gacctgttaa aaaacagaga tggcttaatt attggtatct
    23281 gcaacggttt tcaggcactg ataaaattag gtctggttcc ctttggtgaa atcacaggga
    23341 tggaagagga ctatccaact ttaacatata ataaaatcgg aagacatata tcaacatttg
    23401 tcagaacaaa ggttgtaagt aatttgtccc catggatgag gggatgcaga ctgggagata
    23461 ttcacttact gcctgtgtcc cacggggagg gagcatttgc tgcaaatgaa aaatggattg
    23521 aaagatttaa aacaaacggc cagatagcca ctcaatacgt tgatgaaagc aatgcaccaa
    23581 cctatgacgg ctttaataat ccaaacgggt ctatagaggc aattgagggg ataaccagcc
    23641 ctgacggcag agttctgggc aagatgtccc attcggaaag acttcaggag gatatatata
    23701 aaaacattcc cggaaacaag gaccagagaa tttttgaatc cggagtaaaa tactttaaat
    23761 aacggaggta aaatgaaagt agcaattgtt atgggaagtg attcagactt tcctgttgta
    23821 gaaaaaggtt acaatatttt aaaagaattt ggagtagaaa cagcagtaag agtgatatca
    23881 gcccacagaa ctccttttga agctttggag tttgccagaa atgctaacaa ggacggttat
    23941 gaacttatta tagctgcagc gggaaaggcg gcgcatttac caggtgtgct ggcaggatgc
    24001 acgaccttgc ccgttatagg aataccggtt aaatcatcgg aactggacgg aatggatgct
    24061 ttgttgtcaa tagtgcagat gcctccggga gtgcctgttg caactgtagg cataaattct
    24121 gctgaaaatg cggcgctttt ggctattcag atactgtcgg taaaatacga agacctgaga
    24181 cataaatttt cggcctacaa aaaacaaatg gctgaaaaag tatttgaaaa agacaaaaaa
    24241 ttaaacgaaa aattaatgta aggagaaacc atgtatttag atgattacgg tttaaatgaa
    24301 gataaactgc acgaggaatg tggagttgtg ggagtatata cggacaatcc ggatatagca
    24361 agccagctga tatactacgg tttgttcgcc ctccagcaca gaggccagga aagtgcagga
    24421 attgccataa atacaggaaa aaaaatcgaa tttcacaagg atatgggatt ggttgcagat
    24481 gttcttaatc aggaaattat agacagatta attggaaaca ttgcaatagg acatgttaga
    24541 tactccacat acggagatag cgaaaagcaa aatgcacaac ccctggtagc aaggtacagg
    24601 gaagggtcaa tagcattagc ccataacgga aatttggtga atgctggatc cctgagagat
    24661 atgcttgaaa atgaaggtgt aatatttcag acttcaactg attctgaggt tgtagtcaat
    24721 ttggttgcaa gacattacag gggcagcata gttaaatcca tccaaactac catggaggta
    24781 gttaaaggag catatgcatt tgcaatcatg acggaaaatg aactgatagg agtcagggac
    24841 aactatgggc tgagacctct ttgtctgggg aaaagagagg acggatatct tttagcttcc
    24901 gaaagctgcg gaattgccgc tatgggaggt gaattcataa gagatatcga gcccggcgaa
    24961 attgttgtta taaatgagga cggagttaac agcatcaaaa ccacaaaata cgcccaaaag
    25021 aaattatgca tatttgaaca tgtatatttt gcaagaccgg acagtaattt ggacggcgta
    25081 aatgtatatc agacaagata caattcaggc aggcttttgg cacaggaagc acctgtaaaa
    25141 gcagaccttg tattttcagt gcctgactcc ggaacaccgg cagcattggg ttatgcggca
    25201 gaattaaata ttgcctatgg tcttggaatc ataaaaaaca gatatgcaaa aagaactttc
    25261 atagagccga aagaagtatt aagacaaaag ggagtaaaaa caaaactaag catattgaag
    25321 gatattgtac aggataaggt ggtagtcatg gttgatgaca gcatagtaag aggtacgaca
    25381 accaagcaga tagttaaaat ggtaaaggaa gcaggcgcaa gggaagttca tgttcgaata
    25441 gcttcacctc ctgtaaccca ttcttgcttc ttcggcataa acacacctga caggagcaaa
    25501 cttataggag caaataaaac aaccgaagaa ataaaagtgc atatagaagc tgacagctta
    25561 cattttctaa gcttggaaag tttgttaaag gcaacaggct caacacaagg ttactgtaaa
    25621 gcatgtttaa acggcgatta ccccatggaa gtgccaaaca acggaatata ggaggtatta
    25681 tggataaact gacttataaa gattcaggag tagatgtaaa ttccggttat gaaactgtaa
    25741 ggcttataaa agactatgtt aagaaaaccc atacaaaggg cgtgatttca aatattggcg
    25801 gctttggggg catgtttgaa ataaacaagg acgaatatga agagcctgta ttggtttccg
    25861 gcaccgacgg tgtgggaaca aagcttatgg ttgcatttat ggcagacaag catgacacaa
    25921 tcggagaaga tgctgttgcc atgtgtgtaa atgatgttat atgtcagggt gcaaaaccat
    25981 tattttttct ggattatatt gcaacaggaa aactgaagcc tcaaaaggca gcaagcattg
    26041 tcaaaggcgt ggctgagggc tgcgttaagg ccggctgtgc cttgatcggg ggagaaactg
    26101 cagaaatgcc gggactttac ggtgaagaag aatatgatat tgcgggattt tgtgttggaa
    26161 tcgttgataa aaagaaaatt atagacggct caaaaataat tgagggagat gttcttatcg
    26221 gaataccttc cagtggaatt cacagcaacg gatattcttt ggttcgaaag gtttttttca
    26281 gtcataaaaa ttatgatgtc aaccagtatg tggaggaatt aggatccacc atgggtgaag
    26341 aattattaag acccacaaga atttatccaa agcttatact tgagctgata tcaaagtttg
    26401 aaataaaggg tttgtcaaat attacaggag gaggttttta cgaaaatatt ccaagaatgt
    26461 ttcctgaagg aataacagca gatattgaca ctgaaaatat caatgtactg ccgatattta
    26521 agcttatgca aaaggaagga aatctagata ttgacgaaat gtatggtacc tttaacatgg
    26581 gtatcggaat tgttctggct cttggaaaag atattttgga tnnnnnnnnn nnnnnnnnnn
    26641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26701 nnnnnnnnnn nnnnnnnnnn ntacctttaa catgggtatc ggaattgttc tggctcttgg
    26761 aaaagatatt ttggatgatg ttatggactg gttaaaaagc gtcggggaaa gacctgcagt
    26821 tattggaaga actgtcaaaa gggaaggtgg gctgaaaata tgccacaggt gaaaataggc
    26881 gtattgattt caggttcggg aaccaacctc cagtccttga tagataatac ggagaaggga
    26941 aatattaatg gtataataac agttgtcata tcaaacagaa aggatgcctt tggtttggaa
    27001 agggcaagac ggcacggtat tgacgctgta tatgtaaggc agaaggacta cgaaagcttt
    27061 gacaagttca atgatgcaat tactgacgag ttaaaaagcc ggggtgtaga gctggttgta
    27121 ctggcaggat atttaaaaat tttaactcgt aaatttatag aagagtataa aaaccgtata
    27181 atcaacatac atccatcatt gatacctgcc ttttgcggtg ggggatatta cggcatcaag
    27241 gtgcatgaag aagcaataaa ctacggtgtt aagctaagcg gtgccacagt tcattttgtg
    27301 gatgaaggag cagatacggg tccaataatc atacaggaag ccatagaagt aagctgtgaa
    27361 gatacagccg aaacacttca gcagaaggtg ctgaaaatag agcataaaat actgcctttg
    27421 gcagttaagt attactgtga agggaaaata caaataagcg gaagaaaagt taatattaag
    27481 gagtgaaaaa atgagagctt taataagtgt gtcagataaa agaggaatag ttgaatttgc
    27541 ccgggaattg gaaaatctgg gtattgaaat tatatcaacg ggaggcaccc aaagagcctt
    27601 acaggaggcg ggggttaagg taatagggat ttccgaggtc actaattttc cggaatgcct
    27661 ggatggaagg gtgaaaaccc tgcatcccaa aattcatgcg ggtattctgg caatgcgaaa
    27721 aaaccctgag catatgaaac agctttcaga gctgaatatt gaaacaatag acctggtggt
    27781 tgtaaacctg tatcctttta agcagacaat attaaaggac ggggttacaa ggcaggaagc
    27841 aatagagaat attgatatag gcggccctac aatgctccgg tctgcagcaa agaactatca
    27901 ggatgtagct gttctagttg accctgaaga ttacggcatt gttctcgaag aattaaagga
    27961 gaacaaggga gtttctttag atacgaaatt ttatttatgc tccaaggttt ttgctcatac
    28021 tgctcattat gacaccttga tagcttccta tatgaaaaaa gaaagaaaag atagttccct
    28081 gcctgataca tacacaatga cgtatgaaaa gattcaagat atgcgatatg gcgaaaaccc
    28141 acaccagaaa gccgcatttt acaaagaggt tgaaaatgct gaaggcctgt tggcatctgc
    28201 tgtccagctt cacgggaagg aattgtcctt taacaatatt aacgatacaa acggggcttt
    28261 ggagctttta aaagaatttg aagagccatg tgttgttgcc tgcaagcatg caaatccatg
    28321 cggagtagga actgccgaca atatatatga cgcatattta aaagcattcg atgccgaccc
    28381 tgtttcaata ttcggcggaa ttgtagcctt taacagagaa atagatgaaa agacagcaga
    28441 ggaattaaat aaaatatttg tagaaataat tgctgcccct tcctacagcc gggaagctct
    28501 gtcggtactt aaagcaaaga aaaatataag gctcctgcaa atagaaaaca tattacaaaa
    28561 gcagggcgaa tatacctgcg acattaaaaa ggttgcaggg ggtatattag ttcaagagat
    28621 tgattcaaaa ctcttgcctg aagatgaaga attaaaaaca gtaaccggtg ctaagccggc
    28681 tgaacaggaa atgaaagatc tgctcatggc atggaagatc gtaaaacata caaagtcaaa
    28741 cgccatcata attgttaagg acggccagtc cctagggata ggcccaggac aggtaaaccg
    28801 catttgggca tgcaagcagg caattgaaca tgcttatgaa tttttgggga aagattccac
    28861 aaagggagca gctcttgcat cagatgcctt tttccctttc ccggattgcg ctgaggaagc
    28921 agccaaggca ggaataactt caattattca gccggggggc tcgttaaatg atgataaatc
    28981 catagaagta tgcaacaaac acaatatagc tatggttttc acaaatatga gacattttaa
    29041 acattaggag gacctatgat aattcttgta gttggaggcg gagcaagaga gcatgcaatt
    29101 tgctggaaat taaaacaaag caagcagctt tcaaaactct tttgtgctcc gggcaatgca
    29161 ggcatatctc aaatagcaga gtgtgttgac attaaggctg atgagctgga aaaattaact
    29221 gaatttgcgg tagacaacaa ggttgacctg gtggtagtcg gtcctgaagt acccttggta
    29281 gcaggcttgg ttgacatgct taacgaaaaa ggtataaagg cattcggtcc tgtaaaagcc
    29341 ggagccaaat ttgaagggag caaatcctat tcaaaggatt ttatgaagaa atactcaatt
    29401 ccctctgcca gatacgaagt atatacagat tcaaaaaatg caattggaga ccttcacaaa
    29461 tttaaccctc ctattgttgt taaagctgac ggtttggctg caggcaaagg ggtattgata
    29521 tgtgaaacta taaaagatgc agaagaagca attaaaagca taatggacaa aaaacagttc
    29581 ggtgatgcag gagataccct cgttatagaa gaattcctcc ggggaaccga agcttctctt
    29641 ctttgctttg ttgacggaaa aagtataata cccatggaaa gcgccagaga ctacaaaaaa
    29701 gcatatgatg aagacaaggg actcaatacc ggaggcatgg gatgtttttc tccaaacaca
    29761 atatttactg aggaattgaa caaatatatc aggaaataca taatagacag aaccttggag
    29821 ggctttctaa atgaaggcat tgattttaaa ggtgtcttgt tcatagggct catgattgaa
    29881 aacaatcaag ccaagatact ggaatacaac acccgttttg gagatcctga aacagaggtt
    29941 gttctcccca gactaaaatc agatttaatt gacataatgt caaaatgtat cgatggcaat
    30001 ttgtcagagt gtgatttgct gtggaatgat gaaaaatgcg taactgtagt tttagcttca
    30061 ggaggatatc ctgaaagcta tgaaaagggc aaggaaataa caggattaaa tgatgtggat
    30121 gatgatatag taatatttca tggcggtacc aagctgcgtg acaataagat tgttacggac
    30181 gggggcagag ttttggccgt aactgctctg ggaaccacac tagaccaggc aagaagcaag
    30241 gtatataaaa atatccccaa aattcattat gaaaaaatgg agtacagaaa agacatagcc
    30301 aaagtttaaa aattcctgtg acagcacttt tccttctcca aaacaaataa tttcaggtta
    30361 aattctagaa tagactataa tttatagatt taattgaaat ttgatacgta atgctgtttt
    30421 aaataaatgc tttcagagaa attactccga cagcatttat tttttatcat taaggttgga
    30481 caaatgaata tattattaca aagagactaa agggggaagt tatgaatatt catgttgttc
    30541 aaagtggaga aacattgtgg cagattgccg gttattataa tgtcagcata gaggatattg
    30601 ttaacattaa caggttgaat aatcctgaca ttttgctgcc cggccatgca cttttaatac
    30661 caactaacag tataacttat actgtccagc aaggggacac tatttggcag attgcgcaga
    30721 agtttggagt cagcgtacaa caactgctgt gggaaaacag aattatgaat cctgatttga
    30781 tatacccggg atatgtgcta aaaatacctc aaagacccaa aacagaaata gatgtaaatg
    30841 gctttacata ttttctcggg ccggaagctg ttcccgttgt gagagaagca ggaccttatc
    30901 tgacttattt aagtcctttc gcatacctta ttaacgaaga cggaacattg gtaaatattg
    30961 ctgacgaacc ggcaatagct gagtcctata cacaaggggc tgttcccatg atgtcaatag
    31021 taaattttac tgtaaatgtt gaaggccaga acatagcaaa tattgttttg aataatccgg
    31081 acatagtcaa taaactccag gataacataa tcagcataat gagagaaaag ggttaccagg
    31141 gactaaatat tgattttgaa tatgttcttc ctgaggacag ggaagcctac aacagatttt
    31201 tggaaagtac agttcaaaga tttcataatg aaggattctt tgtttccaca tcattagctc
    31261 caaaattaag tgccgagcaa gctggtcttt tgtatgaagc tcacgattat cccgcccatg
    31321 gaaggcttgc ggatttcgtt gttctcatga cctacgaatg gggctggagg ggaggaaggc
    31381 ctagagccat atcaccatta aatgaaataa gaaaagttct tgattatgca gtatcagtaa
    31441 ttccaagaga taaaatatta atgggattcc agatatatgc aagggactgg aaaattcctt
    31501 ttgaagaagg tgatgaagct gcaacattca gcgttcagga tgcaataaac ttagcctatg
    31561 agcacatggc tgaaattcat tatgaatatg aaagccagtc accatatttt cattatactg
    31621 atgcagaagg aattgcccat gaagtatggt ttgaagatgc aagaagcgcc caggcgaaat
    31681 ttgatactgt gaaggaatac ggattaagag gaatcagcta ctggggatta ggatacgaat
    31741 tccctcaaaa ctttcccctc cttggctcta atttccaaat cagaaaatac tagaaacaat
    31801 ttcttcctct tatcaagccg gtacccaatg ggtgggaaca aggctgattg cattattaat
    31861 agaagctgct cttatttaga cagactgaga ttcattgtct tttttatgaa tttaggagta
    31921 agaggtgcca ataaaggtac caggtataaa aagacagcat agggaattgc ccttgcaccc
    31981 acatctatgg ttgcaagggg aactgctatt gctatgctcc aggggaaaag ctcggcaatt
    32041 aaaattacag tgttttcaat gtctagagct aaatggcttt tagtaaggcc atttttttta
    32101 taaatatttt tcatcaaatc ttgagtaagc aatatgggca gtgtttgatt gcagcaaaag
    32161 gcacatgtaa taacgccggt aaagacagta gttatgtaaa caccggtttt tttgcttatt
    32221 ttaaccagag caccctgaat atcattaagc atgcctgttc cttcaaatat acctgaatag
    32281 gaggatgcta tcaatacgac aaatgtaacg gaaaccattg acattaagcc tccgcctgaa
    32341 attattcctg aaagttcagg gtcagtattc atatatccga aaatcatata attgaatatc
    32401 tgctgaaaag aaaaattttg aaaataccga catacaaaaa ttgctgtcag tatacttgaa
    32461 aacattgtaa actgaacatt ttttcttaat gctgccagca ggaagattac aagggcaggg
    32521 agaattgtaa ttggatgaag gttgaaatta acaggtaatt tatctatcag cccgttattt
    32581 gccacattaa tcggattata ttttgataaa ataaaataga cagccacagc tgccacaaaa
    32641 ggcagagcac ctgtaataaa catatttttt atattgtcat ataagtcagt gcctgtaacc
    32701 atagccacta aatttgcact ggaggatacc ggagagcacc tgtccccgaa gaaggctccg
    32761 gatataatag ctcctcctgc tgtataaatg tcgacacctc cgctttttgc cagtacaatt
    32821 aatacaatac ctattgtccc ggcagttcca aagcttgtac caatagcaaa ggaaacaaag
    32881 caggataata aaaaggcata tagaataaag taatttggat tcattatatt tataccgtaa
    32941 taaacaaaaa aagcaattgt tccgcaagcc ctccataaag ctgtaatcat tcctatcaaa
    33001 gcaaaaatag gtattaaaga aagggacttt ttcatacccc ttagtaccat ttttatcaca
    33061 tttcttatac cgtaccctct attaatagct gttatggaaa aagacagcat tcctatcaag
    33121 aggggaacaa gagtgggaat atgctttagg atgctgtaaa ttaaagataa ggtaaaaata
    33181 ataaccgata ttataaagtc cattatagcc tccatttttc aagtaattcc attataatat
    33241 tatttaaaaa aaatacaagt ttaaagatgg tctaaaagat aacaaagctg ttggagcacg
    33301 ggaaaaagtt acaccatatt tggtttttgc caatgggtaa acaatattaa aaaatacatg
    33361 attgatttat attcaaaagt agtatacaat ttatatagag aacgtttgct tatcaaataa
    33421 ttcttataag gaggaaataa tgaataaaga taagttaagc aaaatgaatt tttcaacaag
    33481 ggcggtacat gccggttaca aacctaatga gtatggggct ttagcaactc ccatatatca
    33541 aacttccaca ttcatatttg actccgctga gcagggcggc agaagatttg cattggaaga
    33601 agaaggctac atctacacaa ggcttggaaa tcccacaact tcccaggttg aagacaagct
    33661 tgcatcactg gaaaatgctg aagcagcagt tgccatggct tcaggcatgg gtgccattac
    33721 ttcagtaata tggtctatag taaatgcagg ggatcatatt attgcagcaa aaaccttgta
    33781 tgggtgtaca tatgcttttc tgaaccacgg tttggctaaa ttcggagtag aggttgattt
    33841 tgttgatact tcagaccctg aaaacgtgag aaaagccatg aagcctaata ctaaaatggt
    33901 atatcttgaa agtccagcaa acccgaatat gctgctgact gatatttcgg aagtttcaaa
    33961 gattgcccac gaagttgaag gctgtaaggt tgtagtagat aatacctatt gcactccata
    34021 tttacaaaga cctattgatc tgggtgcaga cgtggtagtt cattctgcaa ccaagtattt
    34081 aaacggtcac ggtgatgtaa ttgcaggatt tgcagccggt aaaaaagaat tcatggatac
    34141 tgtcagatta tttggaataa aagacatgac cggctctgta ataagtcctt ttgatgcata
    34201 cttaatcaac agaggaatga aaacactgga agtcagaatg gaaaagcact gctcaaacgc
    34261 ccagaaggtt gcagaatttc ttgaaagcca tcctgcaata gaatggattg gctatccggg
    34321 actgaaaagc ttccctcaat atgagcttgg taaaaaacaa atgatgctgc caggtgccat
    34381 gattgcattt gaagttaagg gaggacttga agcaggaaga aaacttatga atacagtaga
    34441 gctttgctca ctggctgtaa gtcttggtga tactgagaca ctgattcagc atccggcatc
    34501 catgacccat tcgccttata ctcctgaaga aagagcaatg agcggaattt ctgagggact
    34561 tgtaaggctt tccattggct tggagcatgc agatgatata atagatgatt taaaacaagc
    34621 tttggataaa ttagtgtaaa tcagcaaaca atatattact atttatcagg aggaatatat
    34681 gagagcaaaa gttgacaggg atgcctgcat aagctgcggt ctgtgcgaaa caatatgtcc
    34741 cggtgttttt gaattggatg acgaaagtat ttctacagtg aaggttgacc ctatacctga
    34801 ggaatacgag aattgcacaa aagaagctga ggaagaatgt cctacagatg caattcatgc
    34861 agaataaaat aaaaaatgct gcttcatttc atatagatgg agcagtattt ttgttaaaaa
    34921 aaattctgct ttattacctt tattgagtga ataagatttg cattaaaatg tagaaaaaca
    34981 gattgatatt tgctatacta taactgggtg gtgagcgtat gaaatactta ataggaattg
    35041 atgccggtgg cacaaagtct gagctgattg cttataatct taatgatagt ccgattttta
    35101 atcttatagg aggattcggc aatccttcag taaacctgga gaagactgta aataacctga
    35161 tatcccttat ttatgactgt gttaatgagc ttggcagaga agactgtcag cttatagcag
    35221 ttggaatggc aggtgtggaa acaggcaact atgcagaact gataaaaaaa tatgtggaag
    35281 gtgcttttgt aatcaaaaca atagttttaa atgatgcaga aatggcctgc aaggcatatt
    35341 ttggagacaa ggacggaatt ctggcaattt cagggacagg atcttcctgt tttgtgcaaa
    35401 aagacggtga aggtgaaatg gttggaggat ggggtcatat cctgggtgac gagggaagcg
    35461 gctatcatac tgttatgcag gtttttaaaa atataattca taaatacgac agggctcttc
    35521 cttttgacag cctgagcaag gctttgataa aagaaatagg ggggtctaca cgttcggata
    35581 taatgaattt catttacaac aacgaaaaaa acaaggttgc agcactcttt cccattatag
    35641 ttgactatgc caataaagga gactcatatg ccattttgct tttagaaaat gcaggaagat
    35701 accttgcaga actaactcaa gttgcatata aaaagaaaaa cttcaaagga cctgctaaaa
    35761 taggtcttaa aggaggggtt tttcataaca gctgtttcat gctttcagaa tatatgaatg
    35821 tattacaaga aggcctagac ctttatgacg taatagatga agacatatca gccactaagg
    35881 cagtctgcaa catatataag gccagtaaaa aaggtgctag ctaagcacct tttacagttg
    35941 acaaaccctg ttcatgtcac cctgttacaa aaacatggta ttttgattac ccttcagaat
    36001 gacaaagcat tgccttgcat ccaataattt tcagtcattg ccgaataagt tccctattcc
    36061 aatatttcca aggaggcttg cttctcctcc cataaatact ccttaagatt atttaaccta
    36121 taaatccaat tataggatag caaacatttt cataggttgc aataaaaaca tcttgttata
    36181 taatgtttat atcattataa tagaaacgga tattattgag tgacaaaact tatttgataa
    36241 agcaggataa
//
LOCUS       DAOPUT010000008        35077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_1156_length_35077_cov_20.555137, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000008 DAOPUT010000000
VERSION     DAOPUT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 35077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35077
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_35077_cov_20.555137"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1328
                     /gene="hutI"
                     /locus_tag="PKK75_01715"
     CDS             90..1328
                     /gene="hutI"
                     /locus_tag="PKK75_01715"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNZ82342.1"
                     /translation="MNKLIIKNASELVTCKGNAPKHGKDMSDIGLIKNGCVVIEDDTI
                     LDVGNSEILKNYNEKEYQIIDASNKAVMPGFIDSHTHLIFGGYRADEFSWRLKGDSYM
                     SIMERGGGITSSVRATRNTSLDEFIHLGIKRLDKILDMGVTTVEGKSGYGLDFETETR
                     QLQAMKELNEIHPVDIVPTFLGPHSVLPEYKGKEDGFIDFMIDDVLPKVAEENLAVFA
                     DIFCEKNVFTVDQSRRFLKAAKNAGLKLKIHADEMYQLGGTELAVELGCTSADHLLQA
                     TDEGIRQLSKSNTVATLLPATAFCLKEEYARARYMIDSGCAVSLATDFNPGSCFTNSI
                     PLIIALAALHMNMTIEEIVTALTVNAATAVGRSDVTGSIEKGKKADIIILEYPSIHFL
                     PYHAAVNIVETVIKNGRIVK"
     gene            1405..2019
                     /locus_tag="PKK75_01720"
     CDS             1405..2019
                     /locus_tag="PKK75_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNZ82343.1"
                     /translation="MRNMTVNEFAKVVASDSPVPGGGSIAALCGALSAALAEMVANLT
                     VRKAKYAEYRDEMTDIINKASLLRDELLNYIKEDSLAYNEVTDAYKLPKETEEEKAYR
                     SGKIQEGLKTAASVPLKVAEASFRIFPLAEAVVSRGNKSSVTDGLVGAMMARTGVLSA
                     VLNIRINLDSIKDEEFVKVLKEKADILEEETIKYEKRILKLSPF"
     gene            complement(2330..2929)
                     /locus_tag="PKK75_01725"
     CDS             complement(2330..2929)
                     /locus_tag="PKK75_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNZ82344.1"
                     /translation="MNKLEAKNYNFSNVRYFSPVQLSEHYQLYQNYIKCLNEVKNDLR
                     NDRIYDECNSSYSNIRSAQKAKTFCFDAIKLHELYFENLTGSNNRINGPIESVIVETF
                     GSYHNFRDKFKCIGMSMRGWVLLCYDRYNNEYYIYGQDSHDSGVMLYAEPLIVLDVYE
                     HAYMIDFGTNRNVYIDAFFQNLDFNVINRRLNNILKQSQ"
     gene            3219..>5310
                     /gene="polA"
                     /locus_tag="PKK75_01730"
     CDS             3219..>5310
                     /gene="polA"
                     /locus_tag="PKK75_01730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNZ82345.1"
                     /translation="MKKIMILDGNSLLFRAFYALPPLKTRKGQFTNAVYGFLSMLYKI
                     IDTYSPEYLCVAFDPEKPTFRHEQYKDYKANRQKAPNELVQQFQLIRDVLDVHNIKRV
                     EIEGFEADDVSGTFACAAKGQGAEVYLVTSDKDYLQLIDENVKVVLTKKGITNTVEMD
                     LNSMEEEYGLTPGQFVDLKALMGDSSDNIPGVAGVGEKTALKLMQEYKSLDNLYANID
                     EIKGKLKEKLETDKMQAYMSQTLARIITDIPLEFNIEEYKVKKPDIKKLSELYDELEF
                     RQFKKRLEETVHPEEESQISLFDTDRAETVKNIDIIYIDTEKKAADIKKTINEKAIIK
                     FLFDGERAFLSKALALGISDGENIYYIDFEKTDEKNVLQKLKEIFESSETAIWGHNLK
                     NEIIYLMKNNIQINNIRFDSEIGKYLLNPSDSSYSVDKIAYEFLNIEIPSENQILGTG
                     VKRKTFKEIDLEQKKAYIFNYLNTVNKVKKQITENIDELKMTSLYEKIELPLIEVLAY
                     MEFVGFKVDINVIDKLGVHFLEKIAELEKDIYELAGETFNINSPKQLSVILFDKLGLP
                     VIKKTKTGISTDIEVLERLSTEHEIADKIIEYRSMVKLNSTYVEGLKNEVNKDTGRVH
                     SVFNQTIASTGRISSTEPNLQNIPTRTEEGRELRKAFVPEDDFILCDADYSQIELRVL
                     SHLADEKNLLDAFDQ"
     assembly_gap    5311..5410
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5411..5768
                     /locus_tag="PKK75_01735"
     CDS             <5411..5768
                     /locus_tag="PKK75_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNZ82346.1"
                     /translation="DGYVTTYFGRRRYIPELSSRNFNIRSFGERIALNTPVQGTAADI
                     IKAAMVGVYQRLKKNKMKSRLILQVHDELIVEAYKDELETVKIILAEEMENVVVDFKV
                     RLKSDINTGGSWYDAK"
     gene            5755..6372
                     /gene="coaE"
                     /locus_tag="PKK75_01740"
     CDS             5755..6372
                     /gene="coaE"
                     /locus_tag="PKK75_01740"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNZ82347.1"
                     /translation="MMQNRPRVIVITGSIGTGKSTAVEIIRDLGFTVLDSDKIVHEGY
                     NAGSELYYKIIGHFGKRVLKEEKIDRRKLGRIVFNNEEKLNELNNIVHGYVYDKLKEG
                     IENCCDNVIFLDIPLILETKDTHEPIYDEIWLIYVSEETQRKRLIERALRENKDPEEV
                     LKIIDKQISIEEKVFMVDEVINNEGSVEELKENIKALLEIKNIGW"
     gene            6376..7092
                     /locus_tag="PKK75_01745"
     CDS             6376..7092
                     /locus_tag="PKK75_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82348.1"
                     /translation="MARRKRVYSRRRIRINYGRVALLAAALVVLILLVVKLFSKNTYI
                     SKVEDALEQEITMMSGTLSTVSKIDAAIYENDGIKFTNQHEGIKRLMSFDGSLPEDKD
                     KAEDVKALLENLIRSEKSEEVLSDLPIKEDGYYWIEADIFTRDKVLFITSEKEYNFDL
                     YYDIENKTVYVKEKYHDEFSKKYNKVDFRGYKATDEFIDILENISGVPEPGLEIPGAD
                     KVNPEPGQNTSGTEIPEDKQ"
     gene            7089..8018
                     /locus_tag="PKK75_01750"
     CDS             7089..8018
                     /locus_tag="PKK75_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ82349.1"
                     /translation="MKKIGSYAKQILYDGNRGYLMIFAAGCLWGTIGLFVKLLAGLGA
                     DTSLTAFLRLFMGFWILMPVMLYKGGLSLFRIDKKGLIQCLTLGILSQALFNYSYNIS
                     IGIVGVATASILLYTSPVFVSIMSRFFFNETIGRIKVFALIINITGCFLMVTGGNLSN
                     LKLSAAGIFFGVAAGFLYSLVTIIGKIASGETHPFTLVFYSFFFGWLAMGILTRPWES
                     IIALSGLKFWTYAFGYGLIPTAGSYLLYMGGLRKKLELSKVPVIASVEVVVATLIGII
                     AFKEKINLISVLGIMILLSSIALMNVAAPGTEK"
     gene            8126..9415
                     /gene="kynU"
                     /locus_tag="PKK75_01755"
     CDS             8126..9415
                     /gene="kynU"
                     /locus_tag="PKK75_01755"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNZ82350.1"
                     /translation="MYNFELTRDFARELDKEDKLAAFKDRFYINEGEIYMDGNSCGLL
                     SKDAEETLFEALNAWKELGIGIWTKGGYFLYQDKLGAMMAPLINAQAKEVTCGNSTTV
                     NIHQCILTFYHPTPERNKIIMDDINFPSDRYAVYSDIRARGYDPDECLKVVKSRDGEK
                     IYEDDIIEAMTDDVCIVMLPAVLYRSAQLIDMERIAKEAHKRGIFAGFDLCHSIGVVP
                     HDFKKINPDFAIWCDYKYLNGGPGAIAGLYINEKHFGMEPGLAGWQGNKKETQFNLYQ
                     EFEHASYAGGWQIGTQPILSMAPIEGALRITNEAGIENIREKSLKITAYLMYLIDNKL
                     KKYGFGIGNPREDSVRGGHVALTHEDALRINEAFKANKIIPDFRYPNVIRLAPAPLYV
                     SYEDVYEMIERIIKIMENKEYEQFENKNREGLDNEIN"
     gene            9581..9925
                     /locus_tag="PKK75_01760"
     CDS             9581..9925
                     /locus_tag="PKK75_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNZ82351.1"
                     /translation="MISSDVIRGYIDTMILYLLLNNPSYGYEISKKIRELTDEKYIIK
                     ETTLYSAFKRMESNGYIEAFSGDETYGKPRTYYRITESGKSYYNEKCEEWKLTKEVIE
                     KFIVKENNDEYY"
     gene            9909..10874
                     /locus_tag="PKK75_01765"
     CDS             9909..10874
                     /locus_tag="PKK75_01765"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HNZ82352.1"
                     /translation="MMNIIKNYLDNMFLGLPETEDVNRAKKELLAMMEDKYNELKNSG
                     KTENEAIGIVISEFGNLDELAETLGIKEALENKPDINHVSYEEAENYIEDSRNISPKT
                     ALGVFLCIMSPTILLLLLGLNESGFIQIKEERLVAAGLAALIALVAAGVSYFIRYSSK
                     LEKYEYLEKNVFELDYRTEQMIRNVMAQYEHSYKSTVNTSVILYIFSALPIILTPLLF
                     EIDELTIISVAVTLTIVAFATYNLISKGGIKEACDVLLQQGDYSVETKSNKTWQLISK
                     VFWLAIVAVYLAYSFITNKWGISWVIWPVAGIMYTIISIVAQEKQ"
     gene            11002..12144
                     /locus_tag="PKK75_01770"
     CDS             11002..12144
                     /locus_tag="PKK75_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HNZ82353.1"
                     /translation="MSRGKLSVLESAMLIAGSGLGTGILAIPYAASRTGFFSIVLFII
                     IAYAVSTVLHLLAADLVLHSKNSSQLIGLFKEHLFRGKYENILTLFFFVILAVVLFLN
                     LTIYIIVASDVMATVMPLPDNTAKIIFYLISSVIVFCGIKIIGISEKYSMFFIFLVVL
                     YLSLLSSRNIIRDLDFTFGNPVTALALYGILMFSFSALFSVPQVVNNIENKSKIKFAI
                     AAGIGANALITMIFTIAVLIASEEITSVATIGLSRSIGLVSQTACSIFVLLAMFTSYW
                     SIALAQLDIIHEQTNLNRNLCWLAATLPTLFISIFLPAAYISYIQLAGGSVALIIGCL
                     LLPAYYNSIVNSNDKLILGRIGKSKFLLLFIFVFYLIMALSSFIKI"
     gene            12203..13549
                     /locus_tag="PKK75_01775"
     CDS             12203..13549
                     /locus_tag="PKK75_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82354.1"
                     /translation="MKIALLAPAGAMHRYNGLFHKNLHYAPITLALLASIAIKEFDAD
                     VKIYDETANPIPLDLDVDLIGITCITGTASRCYKFADYYRKRGIKVILGGVHPSLMPY
                     EAMEHADSVIAGLGEDSFIKAIHDIKENRLQKIYFQDENTTIENRPIPRKDFLDKKKY
                     ITLNTVEAIRGCSHTCTFCAYPKAFGTKLYKRPIEDILEEIKTLKGKIVVFPDVNLIS
                     DFTFAKDLFQAMIPLKKWWFGLTTSAIGTDNELLELFEKSGCKGLLIGFESVNQETQK
                     NIHKGVNKVNEYKELMGKLHSHGIMVMGCFAFGSDEDKIDVFKRTVDMCTEAKIDLPR
                     FSVITPFPGTDFYKELEKENRITERDWALYDVEHVVYKPKHMTKRELEEGIAGAWHGS
                     YSVKNIFKRVDLKNITKIGILYFVYFALNIGYRKYAESFKIFDEGIMTDNSDIPDF"
     gene            13564..>14408
                     /locus_tag="PKK75_01780"
     CDS             13564..>14408
                     /locus_tag="PKK75_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82355.1"
                     /translation="MKIAFIRPSMFGERATDAMKPLIFSIIKPLTPAFAKIIFYDEMI
                     EKLPDNINADVIAMTVDTFSAQRAYQLAKIYKKQGRLIIMGGFHPTMVPDECLNFADS
                     VILGEAEDTWSKAIIDIKNNTLKKKYISENNCDLGTVKYDYSVFQGKKYNPVGLVQFS
                     RGCRFNCDFCSINGFYKGKIRIRPIENVVEDIKNTKEKYIFFIDDNLFTDEREARKLF
                     KAMIPLKKKWVCQISIDIGENKELLELMKKSGCLMVLIGFESLNTGNLKQMGKSANIK
                     NIEYD"
     assembly_gap    14409..14508
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14509..15041
                     /locus_tag="PKK75_01785"
     CDS             <14509..15041
                     /locus_tag="PKK75_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82356.1"
                     /translation="NLKQMGKSANIKNIEYDRIIKKIYDAGMMIYGTFVIGYDYDNKD
                     SAMELAEFAMKNSFAIANFNPLMAMPGTRLYDRLKDENRLTCDDWWTNENFRYGDAML
                     YPRGMTGEDLQKGCKNARYLFSSYKSIFKRLFCKANFRSLSNAYLYLALNIISHIQIK
                     NKQGRKLGGIYEDYTD"
     gene            15022..16368
                     /locus_tag="PKK75_01790"
     CDS             15022..16368
                     /locus_tag="PKK75_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82357.1"
                     /translation="MKITLIKPNIGRREHSLYVDEARMEPLQLGVLAALTPADIDVCL
                     YDDRMEPILYDDPTDLVCITVETFTARRAYEISSEYRRRNVKVIMGGMHVMLIPEEAA
                     EHCDSIMIGDAEDKWGEMIEDLRTGVLKKIYTCKPVLIPQEGVITRRDIFKGKGYLPI
                     TLLQFSRGCPYSCNYCASGVYFKNRHYTRNVQEVIQEIKSQERKTLFFVDDNIVGNKE
                     KAKELFRALIPLNVKWVSQASLDMLKDKELMSLMMESGCLGHVIGFESIKQESINTMK
                     KGVNKSYAENKYKDAIEELRSWGLQTWAAFTLGHEDDTLESIRETYEFALKNKFTFAA
                     YNILMPYPGTELYNKLNRENRLLYDSKWWLHPEYKFNYAAYIPKNMTAHELTEASFLC
                     REKYNSFKSIAYRAFDLKTNMKNPYRFITYCIYNPIFKREVFEKQGMNLGYEETGK"
     gene            16365..17699
                     /locus_tag="PKK75_01795"
     CDS             16365..17699
                     /locus_tag="PKK75_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82358.1"
                     /translation="MKITFILPGIGKKKKDSYLKSWLMEPLTIAVLNTLTPKIYEREF
                     FDDRIENIDFNTDSDIIAITVETYTAKRAYEIAKRFKEKGKIIVMGGYHPTLMPEEAA
                     EHADIVVKGNAEGVWEEVLRDIEKGAYKKIYEGNICLDYGIADRGIYSDKTKKYLPIS
                     LVEIGRGCYHQCEFCSIHSYYNQQYSHRSIETIIEEIKLCSNKIFFFVDDSIFSDKEF
                     AKELFTEVAKLNIIWVTQVTLDIGRDEDLLRLMKKSGCEMILIGFESIDPDNLRQMNK
                     SWTTRLGERDELIERIHNNGISIYASFVFGFDYDNEESFEKNLEFCNHHQFFVTAFNH
                     LLLFPNTETYEKFKKEERLSHDKWWLSEGYTFGTISFKPKLVTDDELKNLCRIYKKKY
                     FAFSSIFKRGITLFKRTKRPLINLVYWIQNILFHFEVDKRFGIPVGENLDEK"
     gene            17689..18801
                     /locus_tag="PKK75_01800"
     CDS             17689..18801
                     /locus_tag="PKK75_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82359.1"
                     /translation="MKNKIMQAELSDGTEMLNLIESDSAKGNIELIYTRRPNACLSYQ
                     KEGEAKIGLVKDDEGSILLQIACVVQSYYINGQARRVGYVGGIRKKKDYKGILNWKDI
                     TDFVTQTKCDMFYCSFLEDNKAALDIFSKKRKYIPEILPVCEYTTYIINPDAIKRPID
                     RTNVKYNINSKDNISSKEKDYIFRQIRESDLKDVYKFLNEEGQKYNFAPIAEDLFKQF
                     HGLSIRDCYILEDKSRILAFGALWDQRDYKQYIVTRYTGYMKFFKKFSKFTEIMGYIP
                     IPKENEILNFPTLTMFYSKDNNSHYYNLFLYEIAKEIRKNYKMFIIGMTASHPNDCIY
                     KKIRSIKFKSSIYYANNDMEIILDHNKPVHIECGLL"
     gene            18987..19865
                     /locus_tag="PKK75_01805"
     CDS             18987..19865
                     /locus_tag="PKK75_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82360.1"
                     /translation="MHYKEYKSILSAKNGMNIYRGCSHGCIYCDSRSLCYQINHDFTD
                     IEIKLNAPEMLEHALKHKRKKCMIATGSMCDPYIHIEDEIMYTRKCLQIIDKYGFGLS
                     ILTKSNKILRDLDILKSINKKTKCVVQMTLTTADENLCRIIEPNVSTTRERFEALKIF
                     RNNGIATVVWLSPFLPFINDTEENLRELLNYCIEAEVHGIMCFGIGLTLRDGNREYFY
                     KKLDEHFSGMKQKYIKEFCNSYECHSKNNNKLMNILYSECYKHNILCKPDKVFSYLSE
                     FEDKQLNEQISLQMLF"
     gene            20003..20467
                     /locus_tag="PKK75_01810"
     CDS             20003..20467
                     /locus_tag="PKK75_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNZ82361.1"
                     /translation="MIIQKNKFDIIIDIICLLCLIGLVLYLAIAWPNIPDQIPGHYDA
                     RGEIDGMTGKSLLIFLAFMAWVMYILIFIAELFPKTWNTGVKVTRENMGRVYRTVKSM
                     LGIIKLQMVLLFTYLTVNTSLAMPLPALFTPLTMILLFGSLAYFMIRLIRVR"
     gene            complement(20809..21915)
                     /gene="msrB"
                     /locus_tag="PKK75_01815"
     CDS             complement(20809..21915)
                     /gene="msrB"
                     /locus_tag="PKK75_01815"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNZ82362.1"
                     /translation="MKIFLLFLTLISVIFLFACDNNTKSSLNNELPDENNINSKGKDD
                     DVMDKDLNGNKLIYLAGGCFWGTEKYLGLVKGVVETEVGYANGNTENPSYEDVCRRNT
                     GHAETVKVVYDPEVIDLENLLKLFYKAIDPVAVNRQGYDIGTQYRTGVYYVDKEDEAV
                     INTSLDELQNSYSEPLAVEVVELKNYYPAEDYHQKYLEKNPGGYCHIGESEFCELEEA
                     QKDTEYKVKSKKELEMTLTEMQYNVTQNSATEPPFDNEYFDNFEEGIYVDITSGEPLF
                     TSKDKFESGCGWPSFSKPIDEDVIEEKKDKSFGMIRTEVRTANSDSHLGHVFNDGPKE
                     SGGLRYCINSASLEFIPKDEMEAKGYGDLLYLFD"
     assembly_gap    22222..22321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22453..22929
                     /locus_tag="PKK75_01820"
     CDS             22453..22929
                     /locus_tag="PKK75_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNZ82363.1"
                     /translation="MKNLVVYYSRTTNTETVAKEIAKAVDGDMRKIELIKGISFFWAG
                     CTSAMGKQGKIKPFDYDLKGYDNIFIGSPVWAGKTSTPINTFLSNADFTGKNVYIFIT
                     QADDKTPSLVFDSIAARVEAKGGKVADKFFITTDMKNPVTAEQARKPVEEWIKKII"
     gene            23051..23527
                     /locus_tag="PKK75_01825"
     CDS             23051..23527
                     /locus_tag="PKK75_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNZ82364.1"
                     /translation="MKNLVVYYSRTGHTEIVAKEIAKIVKGDIKRIELKKKISIFWAG
                     FNALTKKDGKIKPIDFYLEDYDNIFIGSPVWAGKTSTPINAFLSRSNFTGKNVYVFLT
                     QTNKEAHDFVYESVSSRVKAKGGKVIDTFFIKTVEEIPLTPDQVRKPVEEWIKRII"
     gene            23579..23941
                     /locus_tag="PKK75_01830"
     CDS             23579..23941
                     /locus_tag="PKK75_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNZ82365.1"
                     /translation="MNNIKIKRVYAGPSEEDGYRILADRLWPRGISKEKINIGKWAKE
                     IAPSTELRKTFKHDPETMDEFKIRYYFELDNNVHADEFINLIKDKLNEGNVTLLYAAK
                     NEDYNHANVLKEWIEERI"
     gene            24065..24895
                     /locus_tag="PKK75_01835"
     CDS             24065..24895
                     /locus_tag="PKK75_01835"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNZ82366.1"
                     /translation="MLNIGCHLSVSDGYEKMGKDALKIGANTFQYFTRNPRGSKAREI
                     DVKDVNKLRKLMEEHNFAQIVGHAPYTLNISSADAKTREFAMMVLKDDLIRMEHLPHN
                     YYNFHPGSHVKQGVETGIDYIVNALNEVIKEYQTTTVLLETMAGKGTEIGATFEELKQ
                     IIDGVYLTDKIGVCLDTCHVYDAGYDIVNNLDGVLDEFDKIIGLNRLHVIHLNDSKNP
                     FSSKKDRHETIGNGQIGLDAIAAVINHPKLRKLPFILETPNELDGHEKEIKLLKEQFV
                     "
     gene            complement(24905..25462)
                     /locus_tag="PKK75_01840"
     CDS             complement(24905..25462)
                     /locus_tag="PKK75_01840"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNZ82367.1"
                     /translation="MKRCPWCGNDELYVKYHDEEWGVPVHDDKKHFEFLVLESAQAGL
                     SWLTVLRKRENYRKAYDDFDVKKVAEYDDAKIEELLQNLGIIRNRRKIEASINNAKRF
                     LEIQKEFGSFDNFIWSFVDNKAVKNHWNDISEIPSVSDLSDKVSKELKNRGFKFLGST
                     IIYAHLQATGLINDHLTSCFRFDLV"
     gene            complement(25582..27435)
                     /locus_tag="PKK75_01845"
     CDS             complement(25582..27435)
                     /locus_tag="PKK75_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82368.1"
                     /translation="MYSGRMSGGRNYGMASDEEIKNRPKVTKELIFRIFSYLKPYRIW
                     LFFCIITIVLISVLNVMPAVLTGRIIDEGFIGGDFSLLVMLIAASLGVMIASNLLSLF
                     ENYLNVRISQSITKDMKNNLYAHLQQMAHRFFTVNKQGDIITRMTSDVNGVQSVISTT
                     LTSTVSDVAVLVTSMAAMFQKNLILSLVGILILPLFVIPTKAVGKKRWELAQKTQMKY
                     DDSNQLLNETLSVSGQQLVKLFTNEEEEYKKYSEINEDIMKLRIRENMAGRWFRMAIS
                     TFTNMGPMLIYLVGGVLILKYNTQGLTVGDITVMVTLLTRMYRPVSSLLGVQVEFYRA
                     LILFERIFEYLDMPIEIKSKADAVIPSRIAGDLEFKNVNFHYSKDKEILKDVSFRVPA
                     KSMTAVVGPSGSGKTTVTNLLLRLYDVVDGKITIDGIDIRDLDLKFLRETIGMVTQDA
                     YLFNGTIRENLKYARKNATDDELIAACKEANIYDFISSLTDGFDTIVGNRGIKLSGGE
                     KQRISIARVILKNPGIIVFDEATSSLDSISENLIQEAIEPLLKDRTSMVIAHRLSTIM
                     AADQILVVDKGQIVEQGVHEELLKNGGVYKKLYETQFKHLINNIKGIPEAV"
     gene            complement(27435..27872)
                     /locus_tag="PKK75_01850"
     CDS             complement(27435..27872)
                     /locus_tag="PKK75_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNZ82369.1"
                     /translation="MEQIDIMDYILKLARLAKRRPMGEHRLPRSAYRVLKTIDGEDKV
                     QLSELAEKLDIRLSSLSEIISKMTEHELVDKIKSEEDSRIIYVSVSDKGRTQLRHNEE
                     VYTALKNKVNEALTAEEKRQFAAICEKLIVELDSAGKDEGEYK"
     gene            28163..29278
                     /locus_tag="PKK75_01855"
     CDS             28163..29278
                     /locus_tag="PKK75_01855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNZ82370.1"
                     /translation="MIKIFHTADIHIGMKFSNYPEPVKGYLQEARVDVLDNMIKLANE
                     EKCNLFVVAGDLFDKITGIDKKTKDRVINALRNFQGECVLVMPGNHDYDNGMIDLWDS
                     FSKAVSDKIVFINEERTYSLEDYGLNVLVYPAPCHKKHSTTNNLQWIKEEKTDNALIN
                     IGIAHGALEGLSPDLDNSYFSMSVNELQSIPVDLWLLGHTHITYPDKSPIANEKIFNP
                     GTPEPDGMDCKHRGQAWIITVDDNKKTNARLIQTGTYKFTDRVYTIEDKDDLDTILDE
                     LKKDDPENTIARISLKGSVDEDVFEYRQEIYSSVEKTIIYLVVEDFDFKMKITPEKIH
                     REFSDGSFPQELLLSLSDDEDTLQLAYELIMEVKNDN"
     gene            29268..31688
                     /locus_tag="PKK75_01860"
     CDS             29268..31688
                     /locus_tag="PKK75_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ82371.1"
                     /translation="MIIKKYTSGRFAGIKDVRVEFKDGLNVILGSNESGKSTLVEGIH
                     SVLFKPSRIGRKSAEDKEFHSKFMPYPIGDSIDGEIDLEHKGEIYTLSKEWGEGPSSK
                     LTMPNKIVIKSDISINDTLKDVYIYGEGTYSSVFFLKQYYLKEAINRIVQNKEATGEI
                     SSLLRGTIMELDGVSLEKLGQKIQSEIDTLLKRWDVERNYPENNKGINNPYKVGFGQV
                     VESYYAKERIKQNMARAEEAERKFNEICLQIKDTEEKIEELKNKKESMEILENDVIQR
                     SILEPQIKQLDKEISSLSKINQEWPRSEEKIKQLNSDLIKLNDKFKKLEEEKSLSKKV
                     QEKNTLEKNLIKVDEINEKLKNLNAQISGIKPVTKEDIKTLETNYSCMTKAEAMMKAG
                     VIMAKLNYFPEGKKLLVTKDLVEPQEVSAGDSFTAKGFMKLQSENLIEIEIKSGDIDF
                     NEIRIQYENYNKNYEQLLKTLEVKNLEEAKLNKEKLDGLNRTLESYNLQKSQYLGDFS
                     YEELKDRIKEFGDLSLVRNTAVIESDINTVNTEKIDLISEKKLLESNISKWVEEYKDM
                     DGLFNKTVEIKFSQKEIKASIDKLADLPKEYKTADEFRTILTQIRSSYGNYVNVLAKQ
                     KEEYYRLENELPESTYEELSESLPQAEKLFEKRLEKGKRLLKIQENFENIKLKMDEKS
                     FVPVITAFSDYLNLLTGGNYKAKDIDDDFNLKLENGNETVMPLNLLSSGTYDCVALAL
                     RLAIAEYILGDNKGFLILDDCLVDLDPDRKDTAVKLIGQFAKKHQVIFTTCSPDTANL
                     LGGYLIKI"
     gene            31802..>32478
                     /locus_tag="PKK75_01865"
     CDS             31802..>32478
                     /locus_tag="PKK75_01865"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNZ82372.1"
                     /translation="MSKTTIKALRYYDEIGLLKPEETDKFTGYRLYTTDQLFKLHKIQ
                     SYRQIGLSIDEVKMLLAGKNTEQIFNKRKEEIISEINNAKDRLSRIEFILQKQEEIFM
                     SYSAIIKEVPECLVYSVEFTAPDYDSFFETIPAIGARLEEKYPDLKCSVPEYCIIRYL
                     DGEYKDKDIRVELCEAVEQRKPDFDDVVFKQLEAAKVVSVMHKGPYSRLGEAYAFAMK
                     WIEENGYV"
     assembly_gap    32479..32578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32579..33399)
                     /locus_tag="PKK75_01870"
     CDS             complement(<32579..33399)
                     /locus_tag="PKK75_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ82373.1"
                     /translation="MNENKIAVMTDSCSDVPQTLLERFNIYQLALSINYKAKSYRDRI
                     DITPEEVYKNLEIEIPHTSLPAMGEIHELIEKIIADGYKQIIIPVISSGLSGTYNAIK
                     TVCDQFDSIKTAVIDTKNIALGSGFLSIYASQLVERNLSFEEITKKVEDKIKNSHIYY
                     SLQTLQYLVKGGRLGRVEGAFGSLLQIKPIISCDKDGIYYTVAKVRGRKQSINKLIEI
                     VEEELKDKSNYYLAVCHGYAQDEADKIKERIQSYVDRATIYMEGQISPALGVHTGP"
     gene            complement(33392..33973)
                     /locus_tag="PKK75_01875"
     CDS             complement(33392..33973)
                     /locus_tag="PKK75_01875"
                     /inference="COORDINATES: protein motif:HMM:NF020450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HNZ82374.1"
                     /translation="MEKYSELLEFSKIMSEVKLVRWKDLPYFPIYADQLLQIVKDELS
                     FLQIGDEKLITKNMVNNYVKWKMMPEPVKKKYEKLHIASLIVITILKQVVPITKIKDG
                     IKLQIVLQGSEGAYDSFCEAFEESMQLTFLPILKGQNPYVLEERKIEYEKLAISSITM
                     ALSNKLLTERIIEIRNKSYHNQINYAKEVEENE"
     gene            34231..34995
                     /locus_tag="PKK75_01880"
     CDS             34231..34995
                     /locus_tag="PKK75_01880"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018782910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNZ82375.1"
                     /translation="MKLKGKTAVVTGASSGMGREIAYYFAKEGANVVAVARRTERLNE
                     LVEKTKDFEGKVVAYSADITNSEKVNGMIDEAINQFGRMDILVNNAGIMDDMSAVGDV
                     KDEIFDKVFDLNVKSALYAMRKAVKHFEEVGGGIIINIASVGGLYGGVAGAAYTASKH
                     AVVGLTKNTAFMYALKNIRCNAICPGGIITEIGSTETMKNINMDGAKRTGIGMALNPR
                     MGEATEIATVAVFLASDDASFVNGQCIAVDGGWRAF"
BASE COUNT        11997 a         5954 c         6949 g         9777 t
ORIGIN      
        1 gaagggggac acaccttctc tttcgggata gtctttgagg gtagaaggaa tgtccccgta
       61 cttgaagggg gataaagagg agtaggttta tgaacaaatt gattataaaa aatgcatccg
      121 agcttgtaac ctgcaaggga aacgctccaa agcacggcaa ggatatgtcg gatataggac
      181 ttatcaaaaa tgggtgtgtg gtaatagaag acgacaccat ccttgatgtt ggaaattcag
      241 aaatattgaa aaattataac gaaaaggaat accaaattat tgatgcttca aataaagctg
      301 tgatgcccgg atttatagat tctcacaccc atttgatatt cggaggatac agggccgacg
      361 aattttcatg gagattaaaa ggcgatagct atatgtcaat catggagcgg ggcggaggaa
      421 taacaagctc agtgagagcc acaagaaaca catctctgga tgaatttatc catcttggaa
      481 taaaaaggct cgataaaata cttgacatgg gtgtcaccac cgttgagggg aaaagcggat
      541 acggccttga ttttgaaaca gaaacaaggc agcttcaggc aatgaaagaa ttaaatgaaa
      601 tccatcctgt cgatattgtg cctacatttt taggtcctca cagcgtactt cccgagtaca
      661 agggaaaaga agatgggttc attgatttta tgattgatga tgtcctccct aaggttgcag
      721 aagaaaacct ggcagtcttt gcggatatat tttgcgagaa aaatgtattc acagttgacc
      781 agtcaagaag gtttttaaaa gctgcaaaga atgcaggcct aaaattaaaa atccatgcag
      841 atgaaatgta tcagctgggg ggaactgaac tggcagttga attaggatgc acctcagcag
      901 accaccttct tcaggctact gatgaaggaa taagacagct ttctaagtca aacacggttg
      961 caacccttct tcctgctacg gcattctgcc ttaaggaaga atatgccagg gcaagatata
     1021 tgattgattc agggtgtgct gtttcactgg caactgattt taaccccggg agctgcttta
     1081 caaattcgat tccactaatt atagcccttg ctgcattgca tatgaatatg acaattgaag
     1141 aaatagtaac cgcattgact gtaaatgctg ctacagcagt tggcagaagt gatgtaacag
     1201 gaagcataga aaagggtaaa aaagccgata ttataatatt ggaatatcct tcaattcatt
     1261 ttcttcccta ccatgctgca gtaaatattg tagaaaccgt aataaaaaac ggaaggatag
     1321 taaaataacg aaattgctta acaatagaat agaaaaataa ataatagata aaacatatgg
     1381 caaaatggca aggagggaat caatatgaga aatatgactg taaatgaatt tgcaaaagtt
     1441 gttgcttcag attcaccggt acccggcggc ggcagcattg ccgcactctg cggtgctttg
     1501 agcgctgcac tggcagaaat ggttgcaaat ttaactgtga ggaaggcaaa atatgcggaa
     1561 tacagggatg aaatgaccga tataataaac aaggcttctt tgttgaggga tgaactcctt
     1621 aattatatta aggaagacag tcttgcatac aatgaggtta ctgatgcgta taagcttcca
     1681 aaggaaactg aagaagaaaa agcttaccga agcggtaaaa tccaggaagg tttaaaaaca
     1741 gccgcttctg ttcccttaaa ggttgcagaa gcttcattta gaatatttcc tttggctgag
     1801 gcagttgtgt caagaggcaa caaaagctct gttaccgatg gtttggttgg tgcaatgatg
     1861 gcaagaaccg gagtgctttc agcggttctt aatataagaa ttaatctgga ttcaattaag
     1921 gacgaagaat ttgtaaaggt tttaaaggaa aaagcagata tacttgaaga agaaacaatt
     1981 aaatacgaga aaagaatatt gaaattgtca ccgttttaga tggagggaga cacaccttca
     2041 tggcaagaaa cctgtgggct aagtccatgg acaatgcctc ttttataaaa ggcctgaaat
     2101 caggcctttt tctaataata acatcaagtt tggacagcta ttctgtctgt gttcactagc
     2161 agcctatgtc gtcgcattcg ttaaacaaaa ttaccaataa caagaagaaa aacagtaagt
     2221 ttgatcccat gccacctgtg cctgctaccc tgtttctatt acaaaatccc ataattaacc
     2281 tccttaaata attgtgctta cgatattata tgtacgaagg tttctttagt tactgagatt
     2341 gttttaaaat attatttaaa cgacggttaa taacattaaa atcaaggttc tgaaaaaagg
     2401 catctatgta tacatttcgg tttgttccaa aatcaatcat gtaggcatgc tcataaacat
     2461 ccaatactat caggggttct gcataaagca taacaccgct atcatgggag tcttggccgt
     2521 atatgtaata ttcgttgttg tacctgtcat aacacagcaa aacccagcct ctcatgctca
     2581 ttccgataca tttgaattta tcccggaaat tatggtaaga tccgaaagtt tcaacaatca
     2641 cactttctat ggggccgttt attctgttat tactccctgt taagttttca aaatacagct
     2701 catgaagctt tatggcatca aaacaaaaag tttttgcctt ttgagcagac cgtatgttgc
     2761 tgtaactgga attgcattca tcatatatcc tgtcgtttct caagtcattt ttaacttcat
     2821 tcaggcattt tatataattt tgatataatt gatagtgttc gctcaattga acaggggaaa
     2881 aatatctcac atttgaaaag ttatagtttt ttgcttccag tttattcatt attcatacct
     2941 ccgataatta tattccatta tatgtagggg aaaatgaata tatgaaatca aagttttcct
     3001 ttacccccat agtttttgtt gatataataa agatgcccta accccatttc ttcgaccaga
     3061 gggagaaaga aatgacggta ggtcattcgc aacgagcacc aattttgtgc gacgaaagga
     3121 gcaaacaaaa ttgtgctgcg agattgcgag aaatcatctt ttaaaacctg agatcctttg
     3181 tttcactcag catgacaata aaaaggaagg tgaaactaat gaaaaaaata atgattttgg
     3241 acggaaacag tttgctgttc agagcatttt atgcattacc gcctttaaaa acaagaaaag
     3301 gacaatttac aaatgccgta tatggttttt tgagcatgct ctataaaatt atagatacat
     3361 attctcctga atatttatgt gttgcctttg accctgaaaa acccacattc agacacgaac
     3421 aatataaaga ttataaagca aaccgccaga aggcacctaa cgaactggtc cagcagtttc
     3481 agctcataag agatgttctt gatgtgcaca acattaagcg ggttgagatt gaaggctttg
     3541 aggcagacga cgtatccggg acatttgcat gtgctgcaaa aggccagggg gctgaggttt
     3601 acctggttac cagcgacaag gactatctgc agcttataga cgaaaatgta aaggttgttt
     3661 taaccaaaaa aggcattaca aatacagttg agatggattt aaactccatg gaggaggaat
     3721 acggactgac ccccgggcag tttgtggact taaaggcact catgggggat tcctcggaca
     3781 atatacccgg tgtggcaggt gtaggagaaa aaactgccct taaactcatg caggaatata
     3841 aaagcctgga taatctttat gcaaacattg acgaaataaa gggcaagctg aaggaaaaat
     3901 tggaaaccga caaaatgcag gcttatatga gccagaccct ggcacgtatc attactgaca
     3961 tacctcttga gtttaatatt gaggaataca aggtcaaaaa accggatata aaaaaactgt
     4021 ccgaattata tgatgagctg gaattccgcc agtttaaaaa acgtcttgaa gaaacagtgc
     4081 acccggagga ggagagccaa atatccttgt ttgataccga cagggcagaa actgttaaaa
     4141 atatagatat tatttatatt gatactgaga aaaaggcagc cgacataaaa aaaacaataa
     4201 atgagaaggc aataattaaa ttcctctttg acggggaaag ggcatttctc agcaaggctc
     4261 ttgccttagg catttcagac ggggaaaata tttactatat agattttgaa aaaacagacg
     4321 agaaaaatgt cctccaaaaa cttaaagaaa tatttgaaag cagtgaaacc gcaatttggg
     4381 ggcataattt aaaaaatgaa ataatttatc tcatgaaaaa caacattcaa atcaataata
     4441 tccgctttga ttcggaaata ggcaagtacc tcttaaatcc atcagatagc tcctattcag
     4501 tggacaagat tgcctatgaa tttttgaata ttgaaattcc ttccgaaaat caaatcctgg
     4561 gcaccggagt taaaagaaaa acattcaagg aaattgacct tgaacaaaaa aaggcatata
     4621 tttttaatta tttaaataca gtcaataaag ttaagaagca aataacagag aatattgacg
     4681 agctaaaaat gacctccctg tatgaaaaaa ttgaacttcc cctgattgag gtattggctt
     4741 acatggaatt tgtcggcttt aaagtggata taaatgttat cgataaactg ggagttcatt
     4801 ttctggaaaa aatcgcagag cttgaaaagg acatatatga acttgcaggg gagaccttca
     4861 acattaattc accaaagcag ctttcagtta tcttgtttga taaattaggc ctgcctgtta
     4921 ttaaaaaaac caagacaggc atatcaacgg acattgaagt tcttgaaaga ctcagcacag
     4981 agcatgaaat agcggacaaa ataattgaat acaggtctat ggtaaaatta aattccacat
     5041 atgtggaagg actgaaaaac gaggttaaca aggatacagg cagagtgcat tccgtattca
     5101 atcagacaat tgcatccaca ggaaggatat caagcacaga gccgaaccta cagaatattc
     5161 caacccgcac ggaagaagga agggagctga ggaaagcatt tgtgcccgag gatgatttca
     5221 tactctgcga tgcagactac tcccagattg agctgagagt gctgtcccac cttgcggatg
     5281 aaaagaatct gctggatgca tttgaccagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn ggacggctat gtgaccacct atttcggcag gagaaggtat attccagagc
     5461 tttcatcccg taatttcaac atcaggtcat ttggagagag aattgcccta aacactcccg
     5521 tccagggtac tgctgcggat attataaagg cagccatggt gggcgtttat caaagactta
     5581 aaaagaataa gatgaaatcc aggcttatac tgcaggttca tgacgagttg attgttgaag
     5641 catataagga tgagcttgaa acagtaaaaa taatcctggc ggaagaaatg gaaaatgtgg
     5701 ttgttgattt taaggttaga ttaaagtctg atattaatac aggcggtagc tggtatgatg
     5761 caaaataggc ccagggtcat agtgattaca ggaagcatag gaaccggaaa atccactgca
     5821 gtggaaatta tcagagacct ggggtttacg gttcttgatt cggataagat tgttcatgaa
     5881 ggttacaatg ccggaagcga actgtattat aaaataatag gtcatttcgg caaaagagta
     5941 ttaaaggaag aaaaaataga caggcgcaag cttggaagaa tcgttttcaa caatgaagaa
     6001 aagctgaatg aactcaataa tatagtccac ggctatgtat atgacaaatt aaaagaaggc
     6061 attgaaaatt gttgcgataa tgtaatattt cttgacatac cgcttatatt ggagacaaaa
     6121 gatactcatg agcctattta cgacgaaata tggctgatat atgtttctga ggaaactcaa
     6181 agaaaaagac ttatagaaag ggccctcaga gaaaacaaag acccggaaga agtacttaaa
     6241 attatagata agcagatatc aatcgaagaa aaagtattta tggttgatga agttataaat
     6301 aacgaaggaa gcgttgagga acttaaggaa aatataaaag ctctccttga aataaaaaat
     6361 ataggatggt gacttatggc caggagaaaa agagtgtaca gcagaaggag aataagaata
     6421 aattacggca gggttgccct tttggctgct gcccttgttg ttcttatact gcttgttgtg
     6481 aagctgttca gcaaaaacac atatatttca aaggttgaag atgcattgga acaggaaatt
     6541 acaatgatgt caggcaccct cagcactgtt tccaaaattg atgcagccat ctatgaaaat
     6601 gatggaataa aatttacaaa tcagcatgaa ggtataaaaa gacttatgag ttttgacgga
     6661 agcctgccgg aggataaaga caaggcagaa gatgtgaaag ctttgcttga aaacctcata
     6721 aggtcagaaa aatccgaaga agttttatct gaccttccca tcaaggaaga cggctattac
     6781 tggatagaag cggatatttt tacaagggac aaggtgcttt ttattacttc cgaaaaagaa
     6841 tacaactttg atttgtatta cgacattgaa aataagactg tatatgttaa agaaaaatac
     6901 cacgacgaat tcagcaagaa atacaacaaa gtcgatttcc ggggctataa ggcgaccgat
     6961 gaattcattg atatcctgga aaatatttca ggagttccgg agccgggtct tgaaattccc
     7021 ggggctgaca aagtcaatcc ggagcctggt caaaacactt ccggtacgga aattccggag
     7081 gacaagcagt gaagaaaatt ggcagctatg caaagcaaat tctctacgat ggcaacaggg
     7141 gatatctgat gatttttgca gccggatgcc tgtggggtac aataggactg tttgttaaat
     7201 tacttgcagg ccttggtgca gacacatcat taactgcatt cttgagactg tttatgggat
     7261 tttggatact aatgcccgtt atgctttaca agggtgggtt aagcctgttt agaattgata
     7321 aaaagggact gattcaatgc ttgacgttag gaatattgtc acaagctctt tttaattaca
     7381 gctacaatat tagcattggc attgtaggag ttgccactgc atcaatactc ctgtacacct
     7441 caccggtttt tgtaagtatt atgtcaagat tcttctttaa tgaaacaata gggagaatca
     7501 aggtttttgc attgataatt aatattacgg gatgcttttt gatggttaca ggagggaatc
     7561 tgtcaaattt aaaattatca gctgcgggga tattttttgg tgtggcagct ggttttctat
     7621 acagcctggt aacaattata gggaaaattg cctcaggaga aacacatcca tttactcttg
     7681 tattttacag cttttttttc ggatggctgg ccatgggcat actcacccgg ccatgggaat
     7741 caataatagc cctgtcggga cttaagtttt ggacctatgc attcggctac ggcctcatac
     7801 caaccgcagg gtcatatttg ctctacatgg gagggctgag aaagaaactg gaactatcaa
     7861 aagttccggt aattgcatca gtagaggtag ttgttgctac gcttatcggc atcatagcct
     7921 tcaaggaaaa aataaactta ataagcgtgc ttggaataat gattttgctt tcatcaatag
     7981 cattaatgaa tgtggctgca ccgggcaccg aaaagtgaat taaaactcat gtggctgttc
     8041 cgggcactga aaagttaact aaaactcatg tggctgttcc gggcatagaa aactaaagaa
     8101 ataattaaaa atttaggagg ggacaatgta caattttgaa ttaacaagag attttgcaag
     8161 agaactggac aaggaagata aactggcagc atttaaggac cgcttttata ttaatgaagg
     8221 tgaaatatac atggacggca attcatgcgg gttattgagc aaagatgcgg aagaaacgtt
     8281 atttgaagct ctaaatgcat ggaaggagct tggaataggc atctggacca aggggggata
     8341 tttcttatat caggataaat tgggcgccat gatggcaccg ctgataaacg cacaagccaa
     8401 agaagttaca tgcggcaaca gcactacagt taatattcac cagtgcatac tgacctttta
     8461 tcaccccaca ccagaaagaa acaaaattat tatggacgat attaactttc ccagcgacag
     8521 atatgctgta tacagcgaca taagagccag gggatatgac cctgatgaat gcttgaaggt
     8581 tgtaaaaagc cgggatgggg agaaaattta tgaagacgat ataatcgaag ccatgacaga
     8641 cgatgtatgc atagtaatgc tgcccgcagt tctttacagg tctgcccagc tgatagacat
     8701 ggaaagaata gcaaaggaag cacacaaaag aggaatattt gcagggtttg acctatgcca
     8761 ctcaatcggc gttgttcccc atgatttcaa aaaaataaac cctgattttg caatatggtg
     8821 tgattataaa tacttaaacg gaggacccgg agccatagca ggtctatata taaacgaaaa
     8881 acactttgga atggaacccg gtcttgcagg atggcagggc aacaaaaagg aaacccagtt
     8941 taacttatat caggagtttg aacatgcctc atacgcaggg ggatggcaga taggaaccca
     9001 gcctatcctg tcaatggcac cgatagaagg agccttgaga ataaccaatg aagcaggcat
     9061 tgagaatatc agagaaaaat ctttaaaaat aacagcatat ttgatgtatt taatagataa
     9121 taagcttaaa aaatacggct ttggcatagg aaatccaagg gaagactctg taaggggagg
     9181 gcatgttgcc ttgactcatg aggatgcctt gagaatcaat gaggcattta aagcaaataa
     9241 aattattccg gacttcagat atccaaatgt tataagacta gctccagcac ccttatatgt
     9301 ttcttatgaa gatgtatacg aaatgataga aagaataata aagataatgg aaaacaagga
     9361 gtacgagcag tttgaaaaca aaaaccgcga aggtctggat aatgaaatta actgaccgat
     9421 agtacccccg tccccttgtc atcaaggggg ctttttttat taaaaagatt atttaaatat
     9481 ttttaaaaaa gtacttgacc tgtcgtagta aatgtgctat tattactacg acagacaaag
     9541 taacgccaga cgtagtaaaa tttcaataag aggtgagcac atgataagca gtgatgttat
     9601 tcgaggatat attgatacta tgatactgta tcttttgctt aacaatccat cttatggcta
     9661 tgaaatatca aaaaaaataa gagaactgac tgatgaaaag tatatcatca aggaaacaac
     9721 tctttattct gcatttaaga ggatggaatc aaacggatat atagaggcat tttcgggaga
     9781 tgaaacctac gggaaaccaa ggacatatta caggatcacg gaatcaggca aaagctacta
     9841 taatgaaaaa tgcgaagaat ggaaattgac aaaagaagta atagaaaaat ttattgtgaa
     9901 ggagaataat gatgaatatt attaaaaatt atctggataa catgttttta ggattacctg
     9961 aaactgaaga tgtaaataga gcaaaaaaag aacttttggc aatgatggaa gacaaatata
    10021 acgagttgaa aaacagtggg aaaacagaaa atgaagccat tggaatcgta atatccgaat
    10081 tcggaaacct ggatgagctg gcagaaacat tgggaattaa agaagctttg gaaaacaagc
    10141 cggatattaa ccatgtttct tatgaggaag cagaaaacta tatagaagac tcaaggaata
    10201 tttcgccaaa aacagccctt ggagtatttc tttgcataat gtctccaact attttactcc
    10261 tgcttttagg gctgaatgaa tcaggtttta ttcaaatcaa ggaggaaaga ctggtagcgg
    10321 caggactggc agctttgatt gctttggtag ctgcaggagt ttcatacttc atcaggtatt
    10381 caagcaaact ggaaaaatat gaataccttg agaaaaatgt ttttgaactt gattatagaa
    10441 cagaacagat gattcgaaat gtaatggcac agtatgagca ttcttacaaa tcaactgtaa
    10501 atacttccgt gattttgtat atttttagcg cactgcccat tattcttacc cctttgttat
    10561 tcgaaattga cgaattaacg ataatttctg tagcagttac cttaacaatc gtagcctttg
    10621 caacttataa cttaatcagc aagggcggta ttaaggaagc ctgcgatgtg ctcttacagc
    10681 aaggtgacta ttctgtagaa acaaaatcaa ataaaacatg gcagcttatt tccaaggtat
    10741 tttggctggc aattgtcgct gtttatttag catacagctt cataacaaat aaatggggga
    10801 tatcctgggt tatctggcct gtggcaggaa ttatgtatac cataataagc atagtcgcac
    10861 aagaaaaaca ataagagtac ccccgtcccc ttgttacacg tccccctgta ataaaggggg
    10921 ctttttttga ttgaattatt tttaaaaagt tatatactga ttataataat attttaattt
    10981 ccatcaggag atgaagataa aatgagcaga ggaaaattat ctgtgcttga gagtgccatg
    11041 ttaatagccg gaagcggact tggaacagga atactggcaa taccttatgc agcttccagg
    11101 acaggatttt tcagcatagt attgttcata atcattgctt atgcagtaag caccgtactg
    11161 catttattgg cagcagacct ggttcttcat tcaaaaaatt cctctcaatt gataggttta
    11221 ttcaaggagc atttgtttcg cggtaaatac gagaacattt taaccttatt tttctttgta
    11281 attctggcag tcgttttatt tttaaattta accatatata taatcgttgc atctgatgtg
    11341 atggcaactg taatgccctt gcctgataat actgcaaaaa taatttttta ccttatatct
    11401 tcggtaatag tattttgtgg aataaaaatt atcggaatta gtgaaaagta ttcaatgttc
    11461 tttattttcc tggttgtttt gtacttatct cttttatcaa gtcggaatat tataagagac
    11521 ctggatttta cctttggaaa tccggtgacg gccctcgccc tgtatggaat tctaatgttt
    11581 tcattctctg ctcttttttc agttccacag gttgtaaata atatagaaaa taaaagcaaa
    11641 attaagtttg caatagccgc cgggatagga gcaaatgctt taataacaat gatttttacc
    11701 atagctgtcc tgattgcctc cgaagaaata acaagtgttg caacaatagg gttaagcagg
    11761 tccatagggc ttgtttccca aacagcctgc tcaatatttg ttcttcttgc aatgtttact
    11821 tcatactggt cgatagcact tgcacagctt gatattattc atgaacaaac caacttaaac
    11881 aggaatttat gctggttggc tgcaactctt cctactcttt tcatttcaat atttttaccg
    11941 gcagcctata tttcatatat tcagcttgca ggaggaagtg ttgccctgat aatcggctgc
    12001 cttttactgc ctgcatatta taattcaatt gttaactcaa atgacaaact tatattgggc
    12061 agaataggga aaagtaaatt cttattgtta tttatttttg tattttattt aattatggct
    12121 ttatcttcat ttattaagat ataatttaag taagaccagt ataattaatc tcaaatataa
    12181 ttattaatga aagaaggaca tcatgaaaat agctttgttg gcacctgcag gagcaatgca
    12241 tagatacaat ggtttgtttc ataaaaatct ccactatgca cctataacat tagcacttct
    12301 tgcatcaata gccataaaag aatttgatgc ggatgtaaaa atttatgatg agactgcaaa
    12361 tccgattccc cttgatctgg atgttgattt gattggaatt acatgcatca caggcactgc
    12421 atccaggtgc tataaatttg ccgattatta caggaaaaga gggattaagg ttattttagg
    12481 aggggttcac ccgtctctta tgccctatga agcaatggaa catgctgact cggtgattgc
    12541 agggctggga gaagatagtt tcattaaagc aattcatgat atcaaagaaa acagactgca
    12601 aaaaatatac ttccaggatg aaaacacaac cattgaaaac agaccgattc caagaaaaga
    12661 cttcctggat aaaaagaaat atataacatt gaatacagtt gaagcaatac gggggtgcag
    12721 ccatacatgc accttttgcg cctaccccaa agcattcggt acaaagcttt acaagcggcc
    12781 aatagaggat atactggaag aaataaaaac actaaaggga aagatagttg tatttcccga
    12841 tgttaattta atttcggatt ttacctttgc gaaggacttg ttccaggcaa tgataccctt
    12901 aaagaaatgg tggttcggac tgacaacctc tgcaatcgga actgacaatg agctgcttga
    12961 gttatttgaa aagagcggct gcaagggtct ccttattggc tttgaatctg taaatcagga
    13021 aacacaaaag aatatacaca agggtgtcaa taaggtaaat gaatataagg aacttatggg
    13081 caaactccac agtcatggaa ttatggtaat gggctgcttt gcattcggca gcgatgaaga
    13141 caagattgat gtattcaaga gaactgttga tatgtgcaca gaggcaaaga ttgacctgcc
    13201 gagattttcc gtgataacac catttccggg aacagatttt tataaagagc ttgaaaaaga
    13261 gaacagaatt acggagaggg actgggcttt gtatgatgtt gaacatgtgg tctacaagcc
    13321 aaaacacatg actaaaaggg aactggaaga aggtatagcc ggtgcctggc atggctccta
    13381 ttctgtaaaa aatattttta aaagagtaga tttgaaaaat attaccaaga taggaatcct
    13441 gtattttgtt tactttgctt tgaacatcgg ttacagaaaa tatgccgaat catttaaaat
    13501 atttgatgag ggcattatga ccgacaactc tgatatacct gacttttgaa atgaggcgag
    13561 accatgaaaa ttgcatttat aagaccaagc atgttcggtg aaagagccac tgatgcaatg
    13621 aaacccctga tattctccat aataaagcct ttgacgcctg catttgccaa gataattttt
    13681 tacgatgaaa tgatagaaaa attacccgac aatataaatg cagatgtcat agcaatgaca
    13741 gtggacacat tttcagcaca aagggcatac cagcttgcaa agatatacaa gaaacaaggc
    13801 aggcttataa taatgggagg atttcatcca accatggttc cggacgaatg tctgaatttc
    13861 gctgatagcg taattttggg ggaagctgaa gacacctggt caaaagcaat cattgatata
    13921 aagaataata ctttgaagaa aaaatatata agcgaaaaca attgtgattt agggactgta
    13981 aagtatgatt acagtgtttt ccaaggcaaa aaatacaacc cggtaggctt ggttcaattc
    14041 agccggggct gcagattcaa ctgtgacttt tgttcaatca acgggtttta taaagggaaa
    14101 atacggataa gacctataga aaatgtagtt gaggatataa aaaacacaaa ggaaaaatat
    14161 atttttttca ttgatgacaa tttatttacc gatgaaaggg aagcaagaaa actttttaaa
    14221 gcaatgattc ctcttaagaa aaagtgggtt tgccagataa gcattgatat cggtgaaaac
    14281 aaagagcttt tggagcttat gaaaaaaagc ggctgtttga tggttttaat cggttttgaa
    14341 tctttaaata caggcaattt aaaacagatg ggaaaatcgg caaacatcaa aaatatagaa
    14401 tatgacagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aatttaaaac
    14521 agatgggaaa atcggcaaac atcaaaaata tagaatatga cagaataata aaaaaaatct
    14581 acgatgcagg catgatgatt tacggcacat ttgtaatagg ctatgattat gacaataagg
    14641 acagcgctat ggaacttgct gaatttgcaa tgaaaaattc ttttgccata gctaatttta
    14701 atcctcttat ggcaatgccc ggaaccaggc tttatgacag attgaaggat gaaaatagat
    14761 taacctgcga tgactggtgg acaaatgaga acttcaggta tggggatgcc atgttatatc
    14821 caagagggat gacaggggaa gacctgcaaa agggatgcaa aaacgccagg tatttattca
    14881 gctcatataa gagtattttt aaaaggctgt tttgtaaagc aaattttagg agtttaagca
    14941 atgcctactt atatttagcc ttaaatatta tttcacacat tcaaattaaa aacaaacaag
    15001 gaagaaagct tggtggcata tatgaagatt acactgatta agcccaacat aggaagaaga
    15061 gaacacagcc tctatgtaga tgaagcaagg atggagcctt tgcagcttgg agtacttgct
    15121 gccctgactc cggccgatat tgatgtgtgt ttgtatgatg acagaatgga gccaatcctt
    15181 tatgatgatc ccacagattt agtctgcatc acagtggaaa catttactgc cagaagagct
    15241 tacgaaataa gcagcgaata caggagaaga aatgtaaagg taataatggg aggcatgcac
    15301 gttatgttaa tccctgaaga agctgctgaa cattgtgaca gcatcatgat aggcgatgct
    15361 gaagataaat ggggcgaaat gatagaggat ttaagaaccg gagtattaaa gaaaatctac
    15421 acctgcaagc ctgttttaat accccaggag ggagtaataa caagaagaga tatttttaag
    15481 ggtaaaggat accttccaat aacccttctg cagttttcaa ggggatgccc ctactcctgc
    15541 aattactgtg cctccggtgt ttattttaaa aaccgccact atacgagaaa cgtacaggaa
    15601 gttattcaag aaataaaaag ccaggaaaga aaaacactat tttttgttga tgataatata
    15661 gttgggaata aagaaaaagc taaagaatta ttcagagcct taataccttt gaatgttaaa
    15721 tgggtcagcc aggcaagcct ggacatgtta aaggacaagg aacttatgag cttaatgatg
    15781 gaaagcggat gcttaggcca tgtaataggt tttgaatcca taaaacagga aagcattaat
    15841 accatgaaaa aaggcgtcaa taaatcatat gctgaaaaca aatacaagga tgcgatagaa
    15901 gagctgagaa gctgggggct tcagacatgg gcggcattta cccttggaca tgaggacgat
    15961 actttggaaa gcataaggga aacctacgaa tttgcgctga aaaacaagtt tacatttgca
    16021 gcctacaata ttttaatgcc ttatccgggg acagaactct acaataaact aaacagggaa
    16081 aacagacttt tgtatgactc aaaatggtgg cttcaccctg aatacaagtt caattatgct
    16141 gcctacatcc caaaaaacat gactgcccat gaacttacgg aagcatcatt tttgtgcagg
    16201 gaaaaataca attcttttaa atccatagca tacagggctt ttgaccttaa aacgaacatg
    16261 aaaaatccat acagatttat tacctactgt atttataatc ccatatttaa aagagaagtt
    16321 tttgaaaaac aaggtatgaa tcttggctac gaggagacag gaaaatgaaa atcacattta
    16381 tactgccggg aataggaaag aaaaagaaag actcttattt aaaatcatgg cttatggagc
    16441 ctcttaccat tgctgtttta aacactctta cccccaaaat ttatgaaaga gaatttttcg
    16501 atgaccggat cgaaaacata gacttcaata cggattcaga cataattgca attacagtgg
    16561 aaacctacac tgcaaaaaga gcttatgaaa tagcaaaaag attcaaggaa aaaggcaaga
    16621 ttattgtaat gggaggatat caccccactc tcatgcctga agaggcagct gaacacgcag
    16681 atattgtggt caagggcaat gctgaaggag tgtgggaaga ggttttaaga gatatagaaa
    16741 agggtgcata taaaaaaata tatgaaggta atatatgtct cgactatgga atagcagacc
    16801 gcggcattta ctctgataaa acaaagaaat acctgcccat atccctggtt gaaatcggca
    16861 ggggatgcta tcatcagtgt gaattctgct caatccactc ctactacaac cagcaataca
    16921 gtcacaggag cattgagaca atcattgaag aaatcaaact ctgcagcaat aaaatattct
    16981 ttttcgtgga tgacagtatt ttttcagaca aagaatttgc aaaagaactt ttcactgaag
    17041 tagcaaaatt aaatatcata tgggttaccc aggttaccct tgacatcggc agggatgaag
    17101 accttcttag gcttatgaaa aaaagcggat gcgagatgat tttgatcgga tttgaatcaa
    17161 ttgaccctga taatttaagg cagatgaaca agtcatggac aaccaggctc ggggaaagag
    17221 atgagctcat agaaagaatc cacaataacg gaataagcat ctatgcaagt tttgtatttg
    17281 gatttgatta tgacaatgaa gaaagctttg aaaagaacct tgaattttgc aaccatcacc
    17341 aatttttcgt gacggccttc aatcaccttc ttttgtttcc caatactgaa acctatgaaa
    17401 aatttaaaaa agaagaaagg ctgtcccatg acaagtggtg gctttcagag ggctatacat
    17461 tcggaacaat ttcatttaaa ccaaaattgg ttactgatga tgaactgaaa aatttatgcc
    17521 gtatatacaa gaaaaaatat tttgcctttt cttcaatatt caaaagagga ataacacttt
    17581 ttaaaaggac caaaagaccc ttaataaacc ttgtatattg gattcaaaat attttatttc
    17641 actttgaagt tgataagcgt ttcggcatac cggtaggaga gaaccttgat gaaaaataaa
    17701 attatgcagg cagaattatc tgacggcaca gaaatgctga acttaataga atcagattcc
    17761 gcaaagggga acattgagct tatttatacc agaagaccaa atgcctgttt gtcttaccaa
    17821 aaagaaggag aagcaaaaat cggtcttgtt aaagatgatg aaggcagtat tttgctgcaa
    17881 attgcctgtg ttgtgcaaag ttattacata aacggtcaag caagaagagt cggttatgtg
    17941 gggggaatca gaaaaaagaa ggattataag ggaattctta actggaaaga tattacggat
    18001 tttgtgacac aaacaaaatg tgatatgttt tactgctcat ttttagagga taacaaggct
    18061 gcccttgata tattttcgaa aaaaagaaag tatattcctg aaattttacc tgtctgcgaa
    18121 tatactacct atataataaa tcctgatgca attaaaaggc cgatagatag aacaaatgta
    18181 aagtataata taaattcaaa ggataatata agttcaaaag agaaagatta catattcaga
    18241 caaataaggg aatccgatct taaggatgta tacaaatttc taaatgagga aggacaaaaa
    18301 tataattttg ccccaatagc ggaagactta tttaaacaat ttcacgggct gtcaataagg
    18361 gactgctata tattggaaga taaaagcagg atacttgcct ttggagcctt atgggaccaa
    18421 agggattaca agcaatatat agttacaaga tatacgggat atatgaaatt tttcaaaaag
    18481 ttttcaaagt ttactgaaat tatgggttat attcctattc caaaggaaaa tgaaatttta
    18541 aattttccca cattaactat gttttattca aaagataaca acagccatta ctataattta
    18601 tttctgtatg aaatagcaaa agaaataagg aaaaattata aaatgtttat aataggaatg
    18661 accgccagcc atcccaacga ttgtatatat aaaaaaatca ggtccattaa atttaaaagc
    18721 agcatttatt acgcaaataa cgatatggaa ataatattag accacaataa acccgttcac
    18781 atagaatgcg gcttgctata attccgggga cggtttgatg tgagtgtgga aggttactca
    18841 aaacgttcgc tgacaatagc ctttcggtcc atttaactgt aaaatgggtt atataagtat
    18901 tgaaattttg cacactttat cctcatttag taatggtgtt tatatattaa attcgaacag
    18961 tcttaaaaat aaggcaggtg atagtattgc attataaaga atacaaatcg atactttcag
    19021 caaaaaacgg tatgaatatt tataggggtt gcagtcacgg ctgcatttat tgtgacagta
    19081 gaagcttatg ttatcagatt aaccatgatt ttacagacat agaaataaag ctaaatgccc
    19141 ctgaaatgct cgagcatgca ttaaagcata aacgaaaaaa atgcatgatt gcaacaggtt
    19201 ccatgtgtga tccatacatt catattgaag atgaaatcat gtatacccgc aaatgtctgc
    19261 aaataataga taagtatgga tttggactat caatacttac taaatcaaat aaaatcttga
    19321 gggatttaga tatattaaaa agtataaata aaaaaaccaa atgtgtggtc cagatgaccc
    19381 ttaccacagc agacgaaaat ttgtgcagaa tcattgagcc taatgtttca accacaagag
    19441 aacgctttga agcattaaag atatttcgga ataatggtat agcaacagta gtatggttgt
    19501 cacctttttt accttttatt aatgatacgg aagaaaacct tagagaactc ctaaattact
    19561 gtatagaagc agaggttcat ggaataatgt gttttggtat aggattaaca ttgagagacg
    19621 gaaacagaga atatttttat aaaaaattag atgagcattt ttcaggcatg aaacaaaagt
    19681 atattaaaga attctgtaat tcctacgaat gtcatagcaa aaacaataat aaacttatga
    19741 atatactcta ctcagaatgt tataagcata atatactgtg taagccggat aaggtttttt
    19801 cttatttgag tgaatttgaa gacaagcaat taaatgaaca gataagcctg caaatgttat
    19861 tttaaataag caaacagatg ataaacattt ttaagcttat taactgtaaa tgataccata
    19921 aaagtgtatc actttgatgc tgaatgacag cgaaaaagtt taccacctaa catataaaat
    19981 cctttaaagg agtagtttag cgatgataat acaaaaaaat aagtttgata taataattga
    20041 tataatttgt ttattgtgtt tgattggact tgtattgtat ttagctatag catggccgaa
    20101 tattcctgac caaataccgg gccactatga tgcaagggga gaaattgatg gaatgacagg
    20161 caaaagcctg ttaatctttc ttgcttttat ggcatgggtt atgtatattt taatttttat
    20221 tgctgaattg tttccgaaaa cctggaatac aggagtaaaa gtcacaagag aaaatatggg
    20281 aagggtatat cgtacagtaa agagtatgct gggtataatt aagctgcaaa tggttttatt
    20341 gtttacatat ttaactgtaa acacttcttt ggcaatgccc ttgccggctt tattcacacc
    20401 gttaaccatg attttattat ttggttccct tgcttatttt atgattagat taataagggt
    20461 cagataatgg taccatgcat gacgtctgcc aggcatggtg ccagggtacc gctctttata
    20521 tcaaaatgct caaaatttca agtaatatgt ggtttgtgct gtattaattt cgattattcg
    20581 acaaataact tgaaattctt agcatattct acttaaaaat caactttttc caaatatatt
    20641 tcacacatta cttgaaattt tgagcatttc aaagcttata taacccattt tacagttaaa
    20701 tggaccgaag gctattgtca gcgggtgttt tgcgtaacct tcaacactta gaacgaaaag
    20761 gaaccgtcca tctcctaaaa ttacctgttc cccaaaattt ccaaaatatt aatcaaacaa
    20821 ataaagcagg tctccatagc cctttgcttc catttcatct tttggaatga attccagtga
    20881 agcactgttg atgcagtatc tcaatcctcc tgattctttg ggaccgtcat tgaaaacatg
    20941 gcctaaatgg gaatcactgt ttgctgttct tacttctgtt cttatcatgc cgaaactttt
    21001 gtcttttttt tcttcaatca catcttcatc aatgggcttt gagaagctgg gccatccgca
    21061 tcctgattcg aatttgtcct ttgatgtaaa taaaggctct ccggaagtaa tatcaacata
    21121 gatgccctct tcaaaattat cgaagtattc attgtcaaaa ggaggctccg tagcactgtt
    21181 ttgagtaaca ttatactgca tttccgtaag agtcatctct aattccttct ttgattttac
    21241 cttatactcg gtatcttttt gagcttcttc cagctcgcaa aactctgact caccaatatg
    21301 gcagtagccg ccgggattct tttcaagata cttttggtga taatcttccg caggataata
    21361 attcttaagc tctacaacct ctacagccaa gggctcggaa taactattct gcagctcatc
    21421 taaggatgta ttaattacag cttcatcctc tttgtcaaca taataaaccc ctgtacggta
    21481 ttgagttcct atatcatagc cctgcctgtt tacagctacg gggtcaattg ccttgtaaaa
    21541 cagcttcagc aagttttcca aatcaatcac ttccgggtcg tataccacct ttaccgtctc
    21601 cgcatggccg gtgtttcttc tgcaaacatc ctcatacgaa gggttttcag tattaccgtt
    21661 tgcgtaccca acctcagttt ccacgactcc ctttaccaac cctaaatatt tttcagtacc
    21721 ccagaaacat cctcctgcaa gataaatcaa tttatttccg tttaagtcct tatccatcac
    21781 atcatcgtcc tttccttttg aattaatatt gttctcatcc ggcaattcat tatttaagct
    21841 gctttttgta ttattatcac atgcgaataa aaaaattacc gatattaatg ttaaaaacag
    21901 taaaaatatt ttcattttca tattaccccc gcattctaaa tttaaaccta tattaaaagc
    21961 ttttttatat tatcacaaaa tagtcttaat gtttgattaa atttgtaaga ttattgggga
    22021 cggaaattgg cgaacatgta ttctgagtga ggacgaaaag aaccctccct aaaaacaaaa
    22081 agagcggtcc cgaaaaactt gaaattggag aatatgtttg cagcgtgaga aaaaagaact
    22141 gtcacgaaat acttgaaatt gtagtgtatg tttgctgcgt gaataaaaaa gaactgcccc
    22201 gaaaaacata taataaaggt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22321 ntatgtttgc tgcgtgaata aaaaagaact gccccgaaaa acatataata aaggttgtat
    22381 aagatgttga tttgggtata taattaactt ataaattctg gaaattcaca tgaatataaa
    22441 ggggtaaaaa caatgaaaaa tttagttgta tattattcaa gaaccaccaa tacggaaaca
    22501 gttgcaaagg aaatagctaa agctgttgac ggggatatga gaaagattga gctgataaag
    22561 ggaatcagct tcttctgggc aggatgtaca agtgcaatgg gaaaacaggg gaaaatcaaa
    22621 ccatttgact atgatttgaa aggctacgac aatatattca taggctctcc tgtctgggca
    22681 gggaaaactt caacgccaat caacacattt ctaagcaatg cagatttcac gggaaagaat
    22741 gtatatattt tcatcaccca ggcagatgat aaaacaccta gtttggtatt tgattccatt
    22801 gcagcccgtg ttgaagcaaa aggcgggaaa gttgcagaca aattctttat tacaacagat
    22861 atgaaaaacc ctgttaccgc ggaacaggca agaaaacccg tggaggagtg gattaaaaaa
    22921 ataatataaa tatcacggac atcttttaga gcaggcttgc agccgcggat ttgagaacga
    22981 accgtacctg gaatagacat aataaactca taaaatttta aaattcatat tgaataaata
    23041 ggggagaaaa atgaaaaatt tagttgtata ttattcgaga acaggccata ctgaaatcgt
    23101 agcaaaggaa atagcaaaga tagtaaaagg cgatatcaaa agaattgaac ttaaaaagaa
    23161 aatcagcata ttttgggctg gatttaatgc cttaacaaaa aaggacggaa aaataaaacc
    23221 aattgatttt tatttggagg attatgacaa tatatttata ggctctcctg tttgggcagg
    23281 gaaaacttca acacctatca atgcatttct cagccgctca aatttcacag gaaagaatgt
    23341 atatgttttc cttacccaga caaataaaga agcgcatgat tttgtatatg aatctgtttc
    23401 atcacgtgtt aaggcaaaag gcggaaaggt aatagacaca ttctttatta aaacagttga
    23461 ggaaatccct cttacccctg atcaggtaag aaaacccgtg gaggaatgga tcaaaagaat
    23521 aatatagata ttaaacctct ggaagagtag cgcaaagaat atttagaaag gactgattat
    23581 gaataatata aaaattaaaa gagtatatgc cgggccttct gaagaagatg gttacagaat
    23641 actggcagac cgtctctggc ccaggggaat tagtaaggaa aaaataaaca ttggtaaatg
    23701 ggcaaaagaa attgcaccga gcacagagct gcgaaagact tttaaacatg acccggaaac
    23761 aatggacgaa ttcaaaataa ggtattattt tgaactggac aacaatgtac atgctgatga
    23821 atttattaat ctaataaaag acaaattaaa tgaaggaaat gttacattat tgtatgctgc
    23881 taagaatgaa gattataacc atgccaatgt tttaaaggaa tggattgaag agagaatcta
    23941 atcattgtgt aataaaaagg gaattcaaag ggctgccctc ggaattcctt ttataatcat
    24001 taaaatggtt aaaaaggatt aattgagtaa tgataaagat attatcaaga aagggaaaat
    24061 acatatgtta aatattggat gccacttatc tgtttctgat ggatatgaga aaatgggaaa
    24121 agacgcatta aaaattggtg ctaatacatt tcaatacttt accagaaatc cacgaggaag
    24181 caaagccagg gaaatagacg ttaaagatgt taacaaacta agaaaattga tggaagaaca
    24241 taactttgca cagattgtag ggcatgcacc ctatacgtta aatataagtt ctgctgatgc
    24301 aaagacaagg gaatttgcca tgatggtttt gaaggatgat ttgataagga tggagcattt
    24361 gcctcataat tattataact ttcatccggg tagccatgta aagcaaggcg tagaaacagg
    24421 aatagattat attgtcaacg ctttaaatga agtaataaaa gagtatcaaa ctaccactgt
    24481 attactggaa acaatggcag gcaagggtac agaaataggc gcaacatttg aagagctaaa
    24541 gcaaattatt gatggggtgt atttaactga taaaataggc gtatgccttg atacgtgcca
    24601 tgtttatgac gcaggttacg atattgtcaa taacttggat ggtgtattag atgaatttga
    24661 taaaattata gggttaaaca gattacacgt aattcattta aacgacagta aaaatccatt
    24721 tagcagcaag aaagacagac acgagacaat cggaaacggt caaataggct tagacgcaat
    24781 agctgctgta ataaatcatc caaagcttag gaaattgccg tttatattag aaacaccaaa
    24841 cgaattagac ggtcacgaaa aggaaatcaa actgttgaaa gaacaattcg tataaccatg
    24901 ttgtttaaac caagtcaaac ctgaaacaac tggtcagatg atcattaatt aaccctgtag
    24961 cctgcaaatg cgcatagatt attgtggaac caagaaattt gaaacctctg ttttttaact
    25021 ccttgcttac tttatccgac aggtctgaaa cggaaggaat ctcagatata tcattccagt
    25081 gattcttaac agccttatta tcaacaaaac tccagatgaa attatcaaag ctgccgaatt
    25141 ctttttgaat ttccaaaaat ctctttgcat tgttaattga tgcttcaatt tttcttctgt
    25201 ttcttattat gcctaaattt tgcaataatt cttctatttt agcatcatcg tattcagcaa
    25261 ctttctttac atcaaaatcg tcataagcct tcctgtaatt ttctcttttt ctaaggacag
    25321 tcaaccagct taaaccagcc tgtgcagatt caagaaccaa gaactcaaaa tgttttttat
    25381 catcatgaac gggaactccc cattcctcat catgatactt tacatacaat tcatcgttgc
    25441 cgcaccaggg acatcttttc attattaacc tcgcattatt agacttgtta taaagtatat
    25501 caagttttta aaaagcatgc aatacggaag taaaaatctc ttgtattcta tcacaaaaag
    25561 gaactcttat gggcatggaa attaaaccgc ctcaggaatt ccttttatat tgtttataag
    25621 atgcttgaat tgtgtttcat aaagtttttt atagactccg ccgtttttaa gcaattcctc
    25681 gtggacaccc tgctccacga tttggccttt gtccacaaca agtatctggt cagcagccat
    25741 aatagtagac aggcggtggg caataaccat gcttgttctg tccttaagca gaggctctat
    25801 tgcttcttga atcaggttct cggatattga gtccagggat gatgttgctt catcaaatac
    25861 aataatccca gggtttttta gtattactct ggcaattgat atacgctgct tttcaccgcc
    25921 agacagcttt attccccggt ttccaactat agtgtcaaat ccatcggtaa gagaagaaat
    25981 aaaatcatag atgtttgctt ctttgcaggc agcaataagc tcatcatcgg ttgcattctt
    26041 cctggcatat ttcaggttct cccttatcgt cccattgaaa agataagcat cctgtgtcac
    26101 catgccgatg gtctcacgaa gaaatttaag gtctaaatca cggatgtcga ttccgtcaat
    26161 ggtaatttta ccatccacca catcgtagag cctcaacaag agatttgtaa cagtggtttt
    26221 tcctgaaccg gatggtccta ccacagctgt catgcttttg gcaggcaccc tgaatgaaac
    26281 atcctttaaa atttccttgt ccttggaata gtgaaaattc acatttttaa attccagatc
    26341 tcctgcaatc ctggaaggaa taaccgcatc agccttgctc tttatctcaa tgggcatatc
    26401 cagatattca aatatcctct caaataatat gagagccctg taaaactcaa cctgcacacc
    26461 aagcagtgaa cttaccggcc tatacattct tgttagcagt gtaaccatga cggtaatatc
    26521 tcccacggtc agcccctggg tattatattt taaaatcagc acgccaccaa caaggtagat
    26581 gagcatgggc cccatgtttg tgaaagtgct gattgccatg cgaaaccacc gccctgccat
    26641 gttttcccga atcctcagct tcatgatatc ttcgtttatt tccgaatatt ttttgtactc
    26701 ttcctcttca ttggtaaaga gctttaccag ctgctgcccg ctcacactga gagtttcatt
    26761 taaaagttgg ttggaatcgt catatttcat ctgggttttt tgtgcaagtt cccagcgctt
    26821 tttacccact gcttttgtcg ggataacgaa gagaggcagt attagaatgc ccacaaggga
    26881 caggatcaaa ttcttttgaa acattgcagc catagaagtc accaaaacag ctacatcgga
    26941 aacagtgctt gtgagggttg tggatatgac actctgaact ccattgacat cgctggtcat
    27001 acgtgtgatg atgtcaccct gtttgttgac agtgaaaaat ctatgcgcca tctgctgcag
    27061 atgagcatac aggttatttt tcatgtcctt tgtaatactc tgtgatatcc gcacatttaa
    27121 ataattctca aaaagactca agaggttcga tgcaatcatc actcccagcg atgcagcaat
    27181 aagcatgacc agcagcgaaa aatcaccgcc tatgaaccct tcatcaataa tcctgcctgt
    27241 tagaacagca ggcatcacat tgagcacaga gataagaacg attgttataa tacagaaaaa
    27301 cagccagatc cggtagggtt tcaagtaaga gaaaatgcgg aagataagct cctttgttac
    27361 ctttggtctg ttcttaattt cctcgtcact tgccatgccg taattcctgc cgccgctcat
    27421 gcgtcccgaa tacatcattt gtattctccc tcatctttgc ctgcggaatc aagctctaca
    27481 atcagctttt cgcatatggc tgcaaactgt cttttttcct cagctgttaa agcttcattc
    27541 accttgtttt tcaacgcagt ataaacctct tcattgtgtc tcagctgggt ccgcccctta
    27601 tcggagacag acacatagat tatacgggaa tcttcctcac ttttaatttt gtctaccagt
    27661 tcatgctccg tcatcttaga aattatttca cttagcgacg aaagacggat atctaatttt
    27721 tctgccagtt cactgagctg gaccttgtct tcaccatcta tagtttttaa aaccctgtat
    27781 gcacttctgg gcagccggtg ctcacccatg ggccttcttt tcgccagccg tgccagcttc
    27841 aagatatagt ccataatatc tatttgttcc ataaaaactc ctcctttaaa caatttaggt
    27901 acctaactat attataactt aggtacctaa ctatgtcaag aagaaaattc agtacctggc
    27961 ataaacctgc tgctttaagg attttcactt ttttaattta ttgaaaacta gagcattcta
    28021 ctatataata taaacagaag ctgaaaagga tttgttaaaa tttattgtaa aaaccagtgc
    28081 tgaatttgat tattcgaggg tgggaaaggg gataaaaaaa ggtaagcgga gaggtatctc
    28141 aaaaattaat agaggtggaa aaatgattaa gatttttcat acagcagata tacatattgg
    28201 aatgaagttc agcaattatc cggagcctgt aaaagggtac ctgcaggaag caagagtgga
    28261 tgtgctggat aacatgataa agcttgccaa tgaagaaaag tgcaatttgt ttgtcgttgc
    28321 tggagatttg tttgacaaaa taactggaat agataaaaaa accaaggacc gggtaataaa
    28381 tgcactaaga aattttcaag gtgagtgcgt attggttatg cccggcaacc atgattacga
    28441 taacggtatg attgatcttt gggatagctt cagcaaagct gtctctgaca agattgtttt
    28501 cataaacgaa gagcggacct attccttgga ggactatggg cttaatgttc ttgtatatcc
    28561 ggcaccatgc cataaaaaac attcaaccac caacaacctt cagtggatta aagaagaaaa
    28621 aacagataat gcactaataa acatcggcat tgcccacggg gcccttgaag gtttgtcccc
    28681 ggatttggac aacagctact tcagcatgtc ggtaaatgag ctgcaaagca ttcctgtgga
    28741 cctgtggctg ttagggcaca cacatattac atatcctgac aagtcaccca ttgccaatga
    28801 aaaaatattt aacccaggca ctcccgagcc tgacggaatg gactgcaaac acaggggaca
    28861 ggcttggatt atcactgtcg atgacaacaa gaaaacaaat gccaggctga tacaaacagg
    28921 cacatacaaa ttcacggaca gggtctacac aatagaagac aaagatgatt tagacacaat
    28981 acttgatgag cttaaaaagg atgaccctga aaatacaatt gcccgcataa gcttaaaggg
    29041 cagcgttgat gaggatgtgt ttgagtacag gcaggaaata tacagctccg ttgaaaagac
    29101 catcatatat ttagttgttg aagattttga ttttaaaatg aagataacac ccgaaaaaat
    29161 ccacagggaa ttttcggacg gctcattccc tcaggaactt ctgctgtcac tttcagatga
    29221 cgaagacact ctgcagcttg cttatgagct gattatggag gttaaaaatg ataattaaaa
    29281 aatatacaag cggtagattt gcaggcatta aggatgtaag agtggagttt aaagacgggc
    29341 tcaatgtaat cctgggttca aacgagtcag gaaaatccac cttggttgaa gggattcatt
    29401 cagtgctttt taagcccagc agaataggca gaaaatcagc agaggacaaa gagtttcact
    29461 ctaaattcat gccttatcca ataggagaca gcatagacgg agagatagat ttggagcata
    29521 agggagaaat ctacacatta agcaaggaat ggggagaagg tccttcctct aaactcacca
    29581 tgcccaataa aattgtaata aaaagcgaca taagcatcaa tgacacctta aaggatgtgt
    29641 acatttacgg cgaagggaca tacagcagcg tgttcttcct gaagcaatat tatcttaagg
    29701 aagcaatcaa caggatagta caaaacaaag aagcgaccgg tgaaatcagt tccctcttaa
    29761 gaggcacaat catggagctt gacggggttt cccttgaaaa gctagggcaa aaaattcaaa
    29821 gcgaaattga cactttatta aagagatggg atgtggaaag aaactacccg gagaataaca
    29881 aaggtatcaa caatccgtac aaggtcggct ttggccaggt tgttgaaagc tattacgcga
    29941 aggaaagaat aaaacaaaac atggccaggg cagaggaagc tgaaaggaaa ttcaatgaaa
    30001 tctgcttaca aatcaaggat actgaagaaa aaatcgaaga gcttaaaaac aagaaagaat
    30061 cgatggaaat cttggaaaac gatgtaatcc aaaggtcaat ccttgaaccg cagataaagc
    30121 agcttgataa ggaaatatcc tctctttcga aaattaacca ggaatggccc cgcagcgaag
    30181 aaaaaataaa acagttaaat tcagacctta taaagctaaa tgacaagttc aaaaaactcg
    30241 aagaagaaaa gtcactgtcc aaaaaagttc aagaaaaaaa tactctggaa aagaatttaa
    30301 ttaaagtcga tgagataaat gaaaaactaa agaatctaaa cgcacagata tcaggaataa
    30361 aacctgttac aaaggaagac ataaagaccc ttgaaacaaa ttacagctgc atgaccaagg
    30421 cagaagccat gatgaaggca ggagtaatta tggcaaagct taattatttt cctgaaggga
    30481 aaaagctcct tgtaaccaag gacctggtag agcctcaaga agtcagtgca ggagacagct
    30541 tcaccgccaa gggttttatg aagctgcagt cagaaaatct catagaaatt gaaatcaaat
    30601 caggagacat tgattttaat gaaataagaa ttcaatacga gaattataat aaaaactatg
    30661 agcagctgct aaaaacattg gaagtaaaaa atcttgaaga agcaaagttg aacaaggaga
    30721 aattagacgg cttgaacagg accctggaat catacaattt gcaaaaaagc caatacttgg
    30781 gagactttag ctatgaagaa ctaaaagaca ggataaaaga attcggagac ctgtcccttg
    30841 ttagaaacac ggcagtaatt gagtctgata taaatactgt aaacactgaa aaaattgacc
    30901 tgatttcaga aaagaaacta ttggagagca atatcagcaa atgggttgaa gagtacaagg
    30961 atatggacgg cctcttcaat aaaaccgtag aaataaaatt tagtcaaaag gaaataaaag
    31021 caagtattga caaattggca gaccttccta aagaatacaa gacagcagat gaattcagga
    31081 caatattgac acaaatcaga agtagctatg gtaattatgt aaatgttttg gcaaaacaaa
    31141 aagaagaata ctaccggctg gaaaatgaat tgcccgaatc aacatatgag gagctgtccg
    31201 agtcattgcc gcaagcagaa aaactgtttg aaaaaagact ggaaaaaggg aaaagactct
    31261 taaagataca ggaaaacttt gaaaacatca agcttaaaat ggatgaaaag tcatttgtcc
    31321 cggtaattac tgctttctct gattacttga atttactgac aggcggcaat tacaaggcaa
    31381 aagatataga tgatgatttc aatttaaagc tggaaaatgg aaacgagaca gtcatgcccc
    31441 tcaacctctt gtcctcgggg acatatgact gtgtggcact ggccttaagg ctggcaatag
    31501 cagaatatat actgggagac aacaagggat ttttaatact tgatgactgc ctggtggacc
    31561 tggacccaga cagaaaagat acggcagtaa agcttatcgg tcagtttgcc aaaaaacacc
    31621 aggtgatatt tacaacctgc agcccggata cggcaaattt attgggcggg tatttaataa
    31681 aaatataaaa aattttaaaa aataattatt ttagctgttg actctcctgt taggggatgg
    31741 attaagataa atttacaagg aaggagtgag ttttttgtat cgtatcggtg agttttcaaa
    31801 aatgagcaaa acaacaatta aagctttgcg ttactatgat gaaatagggc tgcttaaacc
    31861 ggaagaaaca gacaaattta caggctacag actttataca acagaccagc tgtttaaact
    31921 tcacaaaatt caatcttaca ggcagatcgg cctgtccatc gatgaagtta aaatgctgtt
    31981 agccggtaaa aatacagaac agattttcaa taagcgcaaa gaggaaatta tctccgaaat
    32041 taacaatgca aaggaccggc tctcccgaat tgaatttata ttacaaaaac aggaggaaat
    32101 ttttatgagt tattcagcca ttatcaagga agtgcctgaa tgtttggttt attctgtgga
    32161 gtttactgcc cctgattatg attctttttt tgaaacaatt cctgctatcg gtgcaagact
    32221 tgaagaaaaa tatcccgatt taaaatgttc ggtacctgaa tattgcatta tccgttatct
    32281 tgacggtgaa tacaaagaca aagatataag agttgagctg tgcgaagccg tagaacagcg
    32341 aaaacctgat ttcgacgatg tggtgtttaa gcagcttgag gccgcaaagg tggtttctgt
    32401 aatgcataag ggaccctatt cccgccttgg tgaagcatat gcttttgcaa tgaaatggat
    32461 agaagaaaac gggtacgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
    32581 tcctgtatgt acaccaagag ccggagaaat ttgtccctcc atatagattg tggccctgtc
    32641 tacataggat tgaattcttt ctttgatttt atccgcttca tcctgagcat atccgtggca
    32701 gacagcaaga taataattac tcttatcttt aagctcttcc tccacgattt caattaattt
    32761 atttatactt tgttttcttc cgcgaacctt tgctactgta taatatattc catccttgtc
    32821 gcaggagata attggtttaa tttgaagcaa gcttccaaat gcaccttcta cacgaccgag
    32881 tcttcctccc tttacaagat actgaagagt ttgcagactg tagtagatat gagaattttt
    32941 gattttgtcc tccaccttct tggtaatttc ctcaaagctt aaatttcttt caactaattg
    33001 tgaagcatag attgataaaa aaccagaccc aagggctata tttttcgtat caatcactgc
    33061 agttttaata ctgtcaaatt gatcgcaaac tgtttttatt gcattatagg taccgctcaa
    33121 tcccgatgaa attaccggga tgataatctg cttgtatcca tctgctatta ttttttcaat
    33181 caattcatgg atttccccca tagcaggcag gctggtatga ggtatttcaa tttctaaatt
    33241 tttatatact tcttcaggag taatatctat tctgtcgcga taggactttg ctttgtaatt
    33301 gatacttagt gccaattgat aaatattaaa tctttctaat aaagtctgtg gtacatctga
    33361 acaggaatct gtcattactg caattttatt ttcattcatt ttcttctacc tctttggcgt
    33421 aatttatttg gttgtgatat gatttattcc ttatttcaat aattctttct gtgagaagtt
    33481 tgttggataa tgccattgta atagaagaaa tagcaagctt ttcgtattca attttacgct
    33541 cttctaaaac gtatggattt tgtcctttta aaatcggaag gaatgtcagt tgcatagatt
    33601 cctcaaatgc ttcgcagaaa gaatcatatg ctccttcact cccctgcaaa acaatctgca
    33661 attttattcc atccttgatt tttgtgatag gcacaacttg tttaagtatg gtaataacaa
    33721 ttaaagatgc aatatgcagt ttttcatatt ttttcttaac cggttcaggc atcattttcc
    33781 atttaacata attgtttacc atgtttttgg taattaattt ttcatcccct atctggagaa
    33841 aagatagttc atcttttact atttgcaata gctggtcagc ataaatgggg aaatacggca
    33901 agtctttcca cctgacaagt ttaacttcac tcataatctt actgaattcc aacagctctg
    33961 agtacttttc catttttcac ctttttagat aactatgata tataattatg gtaactacat
    34021 acatgatatt acaatgttta ttgcatgtca agcaagttct cattctcaat aacaaacgct
    34081 gatgcttttg gattgtattc acatagtaaa ttttaatagt tgacagaaaa tctacctgaa
    34141 ttttgcataa tacaattcag gaagatttgt ttaaagcact ttaaataagg gtattatact
    34201 ataaataatt ttaataaaag ggaggattta atgaaactaa aagggaaaac tgcagtagta
    34261 acaggtgcaa gttcaggtat gggaagagaa attgcttatt attttgccaa ggaaggggca
    34321 aatgttgtag cagttgcacg ccgtactgaa agactgaacg aattggttga aaaaacaaaa
    34381 gattttgaag gcaaggtcgt tgcatattct gcagatataa ctaacagcga aaaagtaaac
    34441 ggcatgattg atgaagctat aaatcagttt ggcagaatgg atatattggt aaataatgca
    34501 ggcatcatgg atgacatgag tgcagtcggt gatgtaaagg atgaaatatt tgataaagta
    34561 tttgatttaa atgtaaaatc agcattgtat gctatgagga aagccgtaaa acatttcgaa
    34621 gaagttggcg gcgggataat aattaatata gcttctgtgg gaggactata cgggggcgtt
    34681 gcaggagcag catatacagc aagcaagcac gcggtagtgg gcttaactaa aaatacggca
    34741 tttatgtatg ctctgaaaaa tataagatgt aatgcaatat gccccggagg tataattaca
    34801 gagataggca gcactgaaac catgaaaaat attaatatgg atggtgcaaa aagaaccgga
    34861 ataggaatgg cacttaatcc acgaatgggg gaagccactg aaattgcaac agttgccgtg
    34921 ttcctggcca gcgatgatgc atcatttgta aatggtcaat gcattgccgt tgacggagga
    34981 tggagagcct tctaataccg gcaagcaaat aaactcgtta cttaaaaaaa tctccctgaa
    35041 ttgtccaaaa tacaattctg ggagatttac tttattt
//
LOCUS       DAOPUT010000009        33869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_1217_length_33869_cov_19.614864, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000009 DAOPUT010000000
VERSION     DAOPUT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 33869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33869
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1217_length_33869_cov_19.614864"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..969)
                     /gene="sdaAA"
                     /locus_tag="PKK75_01885"
     CDS             complement(94..969)
                     /gene="sdaAA"
                     /locus_tag="PKK75_01885"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNZ82376.1"
                     /translation="MFVSTGKKLIEICTQKNIPIWEYTIQEEIEKSESSRQTVTDKMK
                     TTLHVMMSSSTYAQENEVHSVSGLIGGDAYKIKHYADNKNTLTGEFMNRAMARALSSS
                     EVNAAMGRIAACPTAGSCGILPAVVLSAGEKLNKTEDELTNALFTAAGVGIIIAKNAT
                     LSGAEGGCQAECGSAAAMASAAVVEMMGGTPGQALSAAAIVIKNILGLVCDPVAGLVE
                     VPCAKRNIAGTVSALTTADLVMSGVYSKIPFDEVVRAMYKVGRQMPLELRETALGGVA
                     ITPAGLKYKKEIFKD"
     gene            complement(969..1652)
                     /gene="sdaAB"
                     /locus_tag="PKK75_01890"
     CDS             complement(969..1652)
                     /gene="sdaAB"
                     /locus_tag="PKK75_01890"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HNZ82377.1"
                     /translation="MRNFSVFDIIGPIMIGPSSSHTAGAARLAKVAASIAGGRIKRVE
                     FLLHGSFSETYKGHGTDKALAAGILDMEPWDENIKNSLETAEEKGIEIVFTPADLGDV
                     HPNTVRFIITKDDGSVSMITGSSIGGGNILIFDIDGQAVEFTGERPTLLTRYRDLPGV
                     ISKISSILYEENINIANMRVYRNEKGKMANMTLETDSIITEDAMNKIRKITEIDNVKF
                     INPIVEGVL"
     gene            complement(1711..>2123)
                     /locus_tag="PKK75_01895"
     CDS             complement(1711..>2123)
                     /locus_tag="PKK75_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ82378.1"
                     /translation="SLKYIMNSNLITVAIPGMDTIEQVVENAGAGIDSEPLTQEEQDI
                     INKEVESLGTDFCRRCGYCKPCPEGIDIPNVFVFENYVIRYKMPEYGKLKYDTLEVKP
                     DQCVRCRKCEKKCPYNLPIVEKLKHAVKTFKELE"
     assembly_gap    2124..2223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2224..2402)
                     /locus_tag="PKK75_01900"
     CDS             complement(<2224..2402)
                     /locus_tag="PKK75_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ82379.1"
                     /translation="MKKRLLGNTGMEVSVIGFGGIPIQHVSEEEAINLILECKRQGIN
                     FIDTARAYTISEKYIG"
     gene            2794..3861
                     /locus_tag="PKK75_01905"
     CDS             2794..3861
                     /locus_tag="PKK75_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNZ82380.1"
                     /translation="MKKNSLIILPVFIIYIAVFRFIYLENGLSLIFQVCFPIFAGILL
                     AAVLNPVLVFFEKTLQIENRYAAVIMTYLFVFLMISVVVKTITPNIIDSIRQLSLDIP
                     KLYREANKLLSHLGDELIVKIYLSEIAQKLSSLLTALINNALTKVIDVFMAFANALLS
                     IIISVYLLIDKEIIEDWIIDVTGIFAGRKTANDIVKIGHILYRNVSSYISGKATASLI
                     MALLIYTGSKYVIKCPYPVIDGIVIGITDMIPYFGTFIGGIPIVLINVLYDPRKGFLL
                     FVLILILQQVESLFIDPKILGSQLSIKPILVIISIIIGGGMFGPLGLFFAVPVAALIK
                     STVDVYMQKKMDKKVRIIDKP"
     gene            3987..4904
                     /locus_tag="PKK75_01910"
     CDS             3987..4904
                     /locus_tag="PKK75_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968903.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNZ82381.1"
                     /translation="MWNDKRYHTLDYELKKIFGEKTIKLSIDGNFTCPNRDDNISSRG
                     CIFCSEKGSGDFTFSGKTITEQIVEQKLFMSKKWKSSTYIAYFQNYSNTYDTVDNLRK
                     KFYEALSCENIKGIAIATRPDCISEEIADFLEGLNHVTFLWVELGLQSIHGKTAEFIR
                     RGFTLEQFNNAVDILKRRNIRTVIHLIIGLPGETPGQMLNSVNYISNLDIWGVKIHML
                     HILKNTDLAEFFEENPFKILTREEYVNLVCDALEILKKEIVVHRLTGDGKKSDLIEPA
                     WTLNKLKVLSDIDKQLKNRGSFQGKKLPL"
     gene            5008..7329
                     /locus_tag="PKK75_01915"
     CDS             5008..7329
                     /locus_tag="PKK75_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ82382.1"
                     /translation="MKKTLLLMIILIFAASFTNLYAVDFPDVKRDFYLEEAIDVLSGY
                     EVISGYPDKTFKPDRIVTRAEMAKMVTVAAGLHEYSKNMTSVYEDMQGHWAESYVELA
                     DVLGIVKGITPSIYGPDNLIKFEEAYTMILRLLGYTDESLIGNWPSNYHEKAVELNLF
                     ENINANVEFASRRDITIMLFNALDMNLVTVRENNTINTTNKTLISKLGKMETREVSIR
                     DLNIDNFDYTDYLFNKWDIYYDNYGAVIHLNNPQYNEFTGTVTSLLSNRVIFVTDDYG
                     NVRVFQVPDIPIVINGERGSFNNLNESKVKIVYEEDSFKGEVLGIIAYKATDVIVVER
                     DDLYKEGSRQFAGKQLPLAGSEINYKKLHIYGDAGSLEDIKVNDVVYFYETRDEENPS
                     LSLNVLRSQVEGIVTSVQNARDNTFYTLNNISYMTGKDYIFTEKASVNDSVTLILDKN
                     NNIIKLNINSYGKRPSTYGIVISSSSSVTGYASARILDEVGGLRTYYLADNSNVVRVQ
                     ENDSGVFKQSLIGSNDLVMFDPVSSGTVKIINLMPDRSIASSYNTQTQTLDNGFYISS
                     DTFIVYRNNGQYQLLVPSQLNGYLEGRAVVNYSGHIDAIYLTKGIKETDAVTVTPDTQ
                     QNYNGTIYGVIKGVTKVDDTTSHVQFFNNSSVFSLSNSSAAGKSISLMRNSYVRAVIV
                     NGVVSSIEKVTAETSKINISQIYSNQMLIDNITYMEYASDLKVFICALDSSGNISSFS
                     EGNISDIKAGSTAQLYDIYGGFDGVIDVVLIYK"
     gene            7661..8791
                     /gene="alr"
                     /locus_tag="PKK75_01920"
     CDS             7661..8791
                     /gene="alr"
                     /locus_tag="PKK75_01920"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNZ82383.1"
                     /translation="MNGKLRPAWVEVNREKAIHNFLEVRRAVGPNVKICAVVKADSYG
                     MGSMELSKMYLENGVDMFAVAVIGEAFELREEIKDKDILVLGFTSEEFYEDAIENNIT
                     LTVYNYDHAVRLNQTAKKLNKKAKIHIKVETGMNRLGFLPTEENADKIADIAKMENIM
                     IEGAFSHQAKADERDKTTAHMQAGRFIKFMGMLEERNVKIPVKHIANSATIIEMPEYY
                     FDMVRPGIILSGFYPSDEVDTEKLKFKICVTLKAKVANVKTIDAGEGVGYGHLYRADK
                     PVVVGTIPIGYADGYSRLLSNKGYVVINGVKCPILGKVCMDQFMVDLSNVENPQIGDV
                     AILYGDGTDGAMTAEDVANMRGTISYEVLTNLAVKRLPKIYI"
     gene            8802..10025
                     /gene="glyA"
                     /locus_tag="PKK75_01925"
     CDS             8802..10025
                     /gene="glyA"
                     /locus_tag="PKK75_01925"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNZ82384.1"
                     /translation="MNYKIIEENDNELFRYMMEEKERQSINIELIASENFVSDTVLQA
                     MGSLLTNKYAEGYPGRRYYGGCEFVDKVENLAIERAKKIFGADHANVQPHSGSNANFG
                     VYFAILKPGDKILGMDLSHGGHLTHGSPVNMSGSYFNVVFYGVNKETETIDYDQVREI
                     AKRERPKLIIAGASAYPRIIDFKVFREIADETGAYFMVDMAHIAGIVAAGLHPNPVDY
                     ADFVTTTTHKTLRGPRGGMILCRKEFAQKIDKAIFPGTQGGPLMHVIAAKAACFNEVL
                     NDGFVEYQKQVLKNAKALSQELMNKGFRIVSGGTDNHLMLVDVRNKNLTGKEAEKILD
                     DIHITTNKNTIPFDPESPFVTSGLRMGTPAVTTKGMKETEMTELADIIDSALSKKAEE
                     DLLRESVLKLTSKFN"
     gene            10266..11387
                     /locus_tag="PKK75_01930"
     CDS             10266..11387
                     /locus_tag="PKK75_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ82385.1"
                     /translation="MVLTLFTACTKSDTTEAEVIKIGVFEPLTGMNGGGGLQELGGIE
                     YANKMYPEVLGKKIELVVVDNKSDKGEATTAVTRLIENDKVVAIIGSYGSGVSIAAGD
                     IIKDAQIPAMGASCTNPMVTQGNDFYFRACFLDPFQGKVMATYAIEQGAKTAAVIMQN
                     GDDYSVGLANFFIDAFKALTGNDASIVDVSEFQLNEADFTAILTNIKAKNPDVIFAPS
                     AATTAPLIIKQARELGIDSLIMGGDTWENPAVIDVAGEDAEGIVLSTFFDENDPATEE
                     AKVFVEGYKKELAGKEPIIPAVAALGYDAYLLVRDAIERAGSTDGVAIRDAIKETKDF
                     EGVTGIINFTEDGDADKNMAVIKTVQDGQFVFIDSVEVQ"
     gene            11457..12350
                     /locus_tag="PKK75_01935"
     CDS             11457..12350
                     /locus_tag="PKK75_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNZ82386.1"
                     /translation="MTSELLLQQLANGISLGSLYALLAIGYTMVYGILRLINFAHGDI
                     FMMACYFMVFSIFTFNLPWYIAIIVVITATVALGVLLEKTAYKPLRNAPRMSIMISAI
                     GASFLLENLATYLFTGVPRGFPDIKVLTATVKLGSLSLTVATLITPFVTLLLLYVVLF
                     ITNKTKIGMAMRAVSKDFETAKLMGININQVISSTFIIGSALAAIGAVLWGTRYPQVM
                     PLMGVMPGLKCFIAAVLGGIGNTTGAVLGGFILGMAEIMLITFLPSLTGYRDAMAFVI
                     LIVVLLVKPTGLLGEKVTDKV"
     gene            12398..13438
                     /locus_tag="PKK75_01940"
     CDS             12398..13438
                     /locus_tag="PKK75_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNZ82387.1"
                     /translation="MIAVFAAVIILLDTGIIGNAYIRRILNLSAIYAIVSVSMNLVNG
                     FTGLFSLGQAGFMAIGAYSVAIFTVPLAERAKIFYITLQNPVLAQIELPFVAALLLGG
                     LLAALVAVLIGLPVLRLKSDYLAIATLGFSEIIRIVFTNTKSITNGALGIKSIPKMPT
                     MWVFFGVAIISIGLMWLLINSSYGRAFKAIREDEVAAEAMGINLFKHKVMSFGVGAFF
                     AGIGGGLLATLLATVDPNQFYFTVTYNFLLIIVLGGMGSVSGTIIASFVVTSGLEILR
                     FFDEPLTLFGANIALFRPGLRMVIFSILLMILVLFFRNGLLGQKELSWKLIFNWLAGK
                     KPIKKTGEGGAA"
     gene            13438..14208
                     /locus_tag="PKK75_01945"
     CDS             13438..14208
                     /locus_tag="PKK75_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82388.1"
                     /translation="MSILKINNVTMKFGGVTAVNNLDIEIQEGKIEALIGPNGAGKTT
                     AFNVITGVYSPTEGNVFYKDENITGLTPDKITKRGIARTFQNIRLFKNLTVLDNILIA
                     NHLRINSNFLSSALRMPWSNKEEKNMREKSEMLLDKLGLLRLKNEIAHSLPYGEQRKL
                     EIARALATDADLLLLDEPAAGMNPSETMELSSFIHTIRDEFKLTIFMIEHHMDLVMEI
                     SEKIYVLDFGQLIARGTADEVKNDQRVIEAYLGVEEDA"
     gene            14201..14908
                     /locus_tag="PKK75_01950"
     CDS             14201..14908
                     /locus_tag="PKK75_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82389.1"
                     /translation="MLKINDLHVNYGGISAVKGISIHVPEKSIVTLIGANGAGKSTTL
                     KTISGLVKARSGEILFQGENILGMDTIDIVAKGITMVPEGRKVFVNLSVLENLKVGAY
                     LRKEGIEEDIKRVYELFPILKERAWQLAGTLSGGEQQMLAVGRALMAKPKLIMMDEPS
                     LGLAPLIVNAIFEIIKEINKQGTTILLIEQNANKALKVADYGYVLETGSMAMEGKGME
                     LLKNEKIIEAYLGTAKK"
     gene            complement(14962..15813)
                     /locus_tag="PKK75_01955"
     CDS             complement(14962..15813)
                     /locus_tag="PKK75_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HNZ82390.1"
                     /translation="MQELVKDALMDSLKMLPFLFAAYIIIEYIEHKSSDRLIIGLRKF
                     GVAGGAVLGCVPQCGFSVAASNLYAGRIISAGTLIAVFISTSDEAIPILLSNNGDIKL
                     ILSLIAVKTVLAVIAGAAADSIFKGLFHISNSEGIASSIHRHVCKDCGCHDEEGILKP
                     AFKHTINIFIFLVVTNFFLNLLITYVGEDNLSGILLNDSIFQPALAGIIGFIPNCAAS
                     VILTQLFISGSLSFGSAIAGLSTGAGAGLLVLFKMNGNIKENIKIMTYIYVFSVSAGF
                     LIQMIMG"
     gene            complement(15996..16274)
                     /locus_tag="PKK75_01960"
     CDS             complement(15996..16274)
                     /locus_tag="PKK75_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ82391.1"
                     /translation="MNKADLISNVAEKTGFTKKDAEKALNGFMETIKEELVAGGKVQL
                     VGFGTFEVRDRKARQGRNPRNPGETIDIPASKAPVFKAGKSLKEAVNK"
     gene            complement(16327..17760)
                     /gene="mazG"
                     /locus_tag="PKK75_01965"
     CDS             complement(16327..17760)
                     /gene="mazG"
                     /locus_tag="PKK75_01965"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNZ82392.1"
                     /translation="MNTIQIIGLGAGTEEDLTLRAKNALSEGIPAFARTDRHPIVNEL
                     RKSIDIVSFDDYFEKYENFDEVYENIIQTLIEQSRRWGKINYCTAGSPYYGDIVTKKL
                     TNEYKSEINTIIIDGMSFLDKCLKLSGYADYNSIKIMDCLEADEFSCDLDSFNIITQV
                     YDIDIASRLKIKLMEIYPEDVNSLIIDVLEENVKKIPLFLLDHEKNYGFSTYFCILPI
                     EISKHRVYNVNNLLRIVKLLRGPDGCPWDKKQTHNSIRQHVIEEAYEVVDAIDNDDVD
                     NLVEELGDLLFQVVFHAELGSEEGYFNFNDVVTNVCKKMYSRHPHVFGNVKAEDVEEA
                     LTSWENSKLKEKNLTTYTDNLRNVPKALSTLSRSYKIQKRAAEVGFDWPDAQGAIMKI
                     KEELLEFIDEYNNGNIENMEEEFGDLLFALVNFARFEKINPDIALNKTINKFLDRFEY
                     IERNSTKDLKNMTLKEMDELWEESKFQ"
     gene            complement(17762..19354)
                     /locus_tag="PKK75_01970"
     CDS             complement(17762..19354)
                     /locus_tag="PKK75_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNZ82393.1"
                     /translation="MSKENFIKGAAILSVSGLFVKILGAIYRIPLTNLIGTEGIGYYQ
                     PAYNIYNLLLVVSLSGFPTAIAKMVSERRALNNCQGAYQVYKTAGWGLFLIGLVSSVF
                     VFLFARSLVTFLGFPGSYYSMVALVPALFVVPLLSAYRGFFQGMQNMTPIALSQLIEQ
                     LFRVAVGLYLAYALVGRGLEEAAAGATFGASAGGLAAFILIFIMFLLNKKNMKEEIAS
                     SKNNKTESARHVIKSLLYIAIPITIGASIAPVMGIADSYIVSNRLSFIGYDDIQIADM
                     FGELSGTAQTLINFPQVFSTAVAMSLVPAITDAFTRRQMNKLSVTADAGVKMSLIIGL
                     PCGIGLFMLAEPIIALLYPSIGPAKHASSGALLEILSIGVIFLTLVQAFTAILQSVNK
                     QFHPVKNLALGLVVKVALSYVLIGIPSININGAAVSTTVAYFFVALFNWLDIKKTKIR
                     INLIKVSSRALLSTAVMALAVWVSFGFIQSAAESRNLATLGSIAAAGIVYVICLFVTG
                     AITKEDLELIPKGEKLKKFVRK"
     gene            19569..21785
                     /locus_tag="PKK75_01975"
     CDS             19569..21785
                     /locus_tag="PKK75_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HNZ82394.1"
                     /translation="MESLKGTIVETIFRNNENGYTVALLETSDEVITLTGIFSTDVSG
                     ENIEVFGERVNHPKYGEQFQVDIYNSILPTGLEQIESYLSSGLIKGIGKYTAKKIVDV
                     FKEDTFDIIQNNPGRLTEIDGIGGKKADMIARSFQEQADVKEVMMFLQSHNISTSYSM
                     KIFKEYGNSTINVIKDNPYRLAEDIYGIGFKLADKIASSLGVEKDSPYRISSGVKYVL
                     AEYAARGHSYVPYGLLVKNSSDLLECSIENTEDEIRNMAFTDKIHIETINSESVVYYL
                     PYHTAEVNVCKNLINLSRPDLSITEEDVLMRLESHEDRSISLTDMQKEAVVQSVLNGA
                     TVITGGPGTGKTTIILSIIKIFEGMNKKVLLCAPTGRAAKRITESTGREAKTIHRLLE
                     YAYGEDDSNLTFNKNENSPLDCHAIIVDEMSMVDILLMNNLLKALKRGTKLIMVGDVD
                     QLPSVGAGNVLSDIIKSNTIKTIRLDMIFRQSLGSMIVQNAHAVNKGVMPILNKKDSD
                     FYFMRESNINNIVDLIVDLYKNRLPSKYGFDPMKDIQVLSPMKKGDAGVIELNKKIQS
                     AVNPPSRLKNEKVFGRYTFRTGDKVMQVKNNYQAEWKLKDDDFITGEGIFNGDMGNIL
                     FIDNVEQEMHVLFDDEKEVIYSFSQLDELILAYATTVHKSQGSEFPVIIMPMVWGPPM
                     LLTRNLFYTAITRAKRLVVLAGQEKYMEEMIENNKIDKRFSALDYRLRTVGQWELA"
     gene            21836..22555
                     /locus_tag="PKK75_01980"
     CDS             21836..22555
                     /locus_tag="PKK75_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNZ82395.1"
                     /translation="MKEYAASFLELLYPEKNTCFICNLYDESINDRYICSDCQKKLKK
                     IMPPLCIKCSKPIDYASECLCPDCAVYEKSFETAKAPFSYEGIVKDSIYSYKYYNKPY
                     FYKFFAACLLNYMKENDYTSFDYITSVPLHRSKMRTRGYNQSELLAKHLSRELSIPYA
                     DALKRTVKTPKQSQQSKDERRKNLKGAFAVKFPLRNENIKNSRVLLVDDVYTTGSTAD
                     ECSKVLLDFGVSKVYVITIAR"
     gene            complement(22588..23166)
                     /locus_tag="PKK75_01985"
     CDS             complement(22588..23166)
                     /locus_tag="PKK75_01985"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNZ82396.1"
                     /translation="MESKKELRKRILSIRNNMPGEDVVNNSRIIRDKIIGLDVYKQSK
                     VIFIYMDFKNEVMTSDLIKHMLFEKKRVVVPYTDTVSTVLIPSEITGESDLKLNSFGY
                     SEPKKVSPVNIEEIDLVIIPGLVFDKNLNRIGFGKGYYDRILNKLKASARKVAVAHDF
                     QVLEEIPAEEHDVKMDMIITEKSIMKRDTSSL"
     gene            complement(23176..23793)
                     /locus_tag="PKK75_01990"
     CDS             complement(23176..23793)
                     /locus_tag="PKK75_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNZ82397.1"
                     /translation="MDFLLGRLLYLPGILLGISVHECSHGYAAVRMGDDTPLMQGRIT
                     LNPLSHLDPMGFLCLLVFGFGWAKPVQINPRNFKNPRRDDALVSLAGPVSNFLVAFLF
                     MGLMKLADMYLPYTLPNQIVFEVLRSTVYINLVLMAFNLIPIPPLDGHHILGSIGGAK
                     VWNFYYKYREQLRFALLLLIVFRGIGRIIGPVINVIYRFLFSIFF"
     gene            complement(23832..25160)
                     /locus_tag="PKK75_01995"
     CDS             complement(23832..25160)
                     /locus_tag="PKK75_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ82398.1"
                     /translation="MLDEGSIWGPLILQTILLFVNAVFASAEIAVISMNDNRIAKLTL
                     EGDKRAVRLTKLTQDSSQFLSIIQIAITLASLLGGAFAAENFAGRLTNLLIKIGLNIP
                     FSVLNPVSVVLITVLLAYITLVMGELVPKRVAMKNAERLALSLSGLLYFIARLFAPLV
                     WLLTVSTNSVLRLIGIDPDAVDDEVTEEDIRMMVDVGSEKGSINENEKEMIQNVFEFD
                     NKTAEEVMTHRTEAVILWLDETEDEWDDKISQSRHSKYPVCDGSADNVIGVLYVKDYF
                     RIEDKSRENIMKNVVKPAYFVPETVRTDVLFQNMKKTRNHFAVVFDEYGGMSGIITMN
                     DLLEQIVGDIDDDYLIPGEEEIEKIDDNTWKIKGSAYLDDVSRELKVSLPDEDFDTFG
                     GLVFGILGIIPEDGSTLELEEYGLRILVTEIKGRRLETSIVYKNLDNGEE"
     gene            25391..25969
                     /locus_tag="PKK75_02000"
     CDS             25391..25969
                     /locus_tag="PKK75_02000"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HNZ82399.1"
                     /translation="MFLEALVLGIIIGFIRRGKLSRLSYVNFSFRPLIYISALFYLGI
                     IVINLGLYDYDSFLYSAFLIGSMVFTGLFLIANIHIRFMFIPLVGLALNLLSFLANTF
                     KFPLSPEATAQIYGQEATELLNAGKLLFYTSSETAVLSFLGSIIPVGNWFVVSIGDII
                     AALGVVLIVQCIMADKFIQNRGKITFSKDIFR"
     gene            complement(26021..>26389)
                     /locus_tag="PKK75_02005"
     CDS             complement(26021..>26389)
                     /locus_tag="PKK75_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNZ82400.1"
                     /translation="AILIAEGQKSSAILVSQGKKESLILEAEAEKQAAILRAEAKKEA
                     SIREAEGEAEAILSIQKATAEGLKMIKDVELNKEVLTFKSFESFEKAANGQATKIIVP
                     SEIQGLAGLSKVLTETIKES"
     assembly_gap    26390..26489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26490..26785)
                     /locus_tag="PKK75_02010"
     CDS             complement(<26490..26785)
                     /locus_tag="PKK75_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNZ82401.1"
                     /translation="MLEYLGFVLLLAIVVVLLAANIRIVPQANAYVIERLGAYQATWS
                     VGVHVKVPLIDRVVKKVSLKEQVIDFPPQPVITKDNVTMQIDTVIFFQITDPKF"
     gene            complement(26797..27228)
                     /locus_tag="PKK75_02015"
     CDS             complement(26797..27228)
                     /locus_tag="PKK75_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNZ82402.1"
                     /translation="MEYIGYAWLGLVVACVIIEAMTVSLTTIWFATGAIVAWLVYLIG
                     FGMEVQIIVFLLVSIVCLIFTRPIAMEKFKVGRVRTNAESLIGQSYKVQSTIDNINNT
                     GTIDVKGQMWSARSVDDQVIEKDEIVIIRSIVGVKLMVEKK"
     gene            complement(27291..28853)
                     /locus_tag="PKK75_02020"
     CDS             complement(27291..28853)
                     /locus_tag="PKK75_02020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ82403.1"
                     /translation="MKKIIFIVILSVLLTGCSTVFSDVTEIEAVNLQNGDNTLIPKES
                     KDADIILDAVNKKDKTEEDISTLLNYEIILKKDKSWEIYKIYFDTGSQAAYIAKDDTI
                     YKIKDNAARQLFLSQVFSYIYVSEIYDSYLELNGEKLSPDVEYNWNSKNIEGHFVNNQ
                     GILKGKVENNNLVSLSQTDVIDIKHEKQPDNQTIRIYDQDKSLLYTGKELGEVLSSIK
                     SDGEYLVECESRWRYKEDSPSYGSRTLKFNALLDIPADINIVTKENYPGNILLISVEN
                     LNTDEAIKLYTDAVKIDNDIYVYKDKNYYIFPIDLYAQPGDYLVTAVVNEGSEREYML
                     EKTLTVGNKSFKTQYLTVSEEMNETNNDNTAIYEFAQLVKPARTDSAKEKLWEGTFVM
                     PVEGELTTDFAEIRYVNNEPSASRHSGLDLAAPKGTPVIAPNNGRVTFAMEGLLSPGN
                     TIVIDHGMGLFTSYYHLNTIGVEKGDQVKKGDTIGTVGTTGFSTGPHLHYAVSIYNTY
                     VNPYQTLSGIID"
     gene            complement(29061..30011)
                     /locus_tag="PKK75_02025"
     CDS             complement(29061..30011)
                     /locus_tag="PKK75_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82404.1"
                     /translation="MLIKFLIAAVFAGTPLIFGTVGEIMNERAGLLNLGVEGMMAMGA
                     VAGFMAGYFYDSILIALLAAFLAGVLGALIYAVLTITFMANQNVTGLTLTIFGVGISN
                     FAGEFMLVNSASSSLKLPEQIASQISNLNIPGLSTIPVIGPLFFQYNIFVYLGLFIAV
                     ICGIYYSRTKTGLNVRAIGENPGAADAAGIKVTKLKYINVLIGGGICGIGGAYCSMII
                     NGGVWMNNPVNGQGWIAVALVIFASWRPLRAIFGSFIFGAFNILKYYFPKTIFTFPNA
                     FYDMLPFIITALVLVITSIRKSKENSQPASCGVNYFREER"
     gene            complement(30013..31095)
                     /locus_tag="PKK75_02030"
     CDS             complement(30013..31095)
                     /locus_tag="PKK75_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82405.1"
                     /translation="MNNKSHTPFIRIVKISERSNKDTINIRIIAFVSALTAGGIFILL
                     LGYNPLELYGTIISGAFRSKMAAQATIRFMIPLLMSSLGVALAFKMRFWNIGAEGQII
                     AGAVCASYFALYHDNWPHFLLLIVMFLAGAIGGGLWGLIPAYFKSKYDTNETLFTLML
                     NYIALYVITYLRDGPWKDPGSSGFPKIARFTANAQIDKVLGVHFGWIIALILVFLVYI
                     YLKYTKHGYEISVVGESKSTARYAGMDVRKIILRTMIISGGICGITGMIQAAGSDMTL
                     ASSVAGGVGFTAIIVSWLANLNPFGILLVSFLFSVLEKGSSVMQSTYGLSTYFAGVLQ
                     GIILFFILGCEFFIRYKFVLTKKGGR"
     gene            complement(31088..32620)
                     /locus_tag="PKK75_02035"
     CDS             complement(31088..32620)
                     /locus_tag="PKK75_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82406.1"
                     /translation="MKTNETNYAIELKGITKTFGKVIANDNVSLGIKYGEILALLGEN
                     GSGKTTLMNILSGIYYADEGTISIDGKEAVITNPNEAINSGIGMIHQHFKLVEVFSAM
                     DNIVLGTKGKYVKSSALKEEILQMSNKFGLEIEPDKKVYNMSVSEKQTVEIMKVLYRG
                     AKILILDEPTSVLTPQETEKLFIILRKMKEQGNAIIIITHKLNEVLAISDRVAILRKG
                     QVVDTVKTADTNEKELTELMVGRPISLEIKRPETENNKTVLRVVDLSVVNEDGTIGLK
                     DINFTLKSGEILGVAGIAGSGQRELCETIAGLMDARKGAVLYNKENIIGKTPDEIINL
                     GISMSFVPEDRLGMGLVGSMDMIDNMLLKSYKKNKGFIIDKKPSRLLAEELVDKLSIV
                     TPGVETPVRMLSGGNVQKVLLGREIESEPNVLITAYPVRGLDINSSYIIYDLLNAQKK
                     KNVAILFIGEDLDVLLELSDRIMVLCHGEIMGIVDAKNTAKEEIGLMMAGKKMKGVDS
                     DE"
     gene            complement(32676..33833)
                     /locus_tag="PKK75_02040"
     CDS             complement(32676..33833)
                     /locus_tag="PKK75_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ82407.1"
                     /translation="MKKLLSIILVLVLALTMLTACGSNEQQPGPQEPGPSGEGVFPAV
                     PKEDIKVGVIHIGNPADGSGYSFAHDQGIVAMQNALGLDDSQIIRKNNVNDTDPTATE
                     AAILECIEEGAHIVFGTSWGYMDTMEALAAEYPDVIFSHGSGYKGNDRNFNNYFGRIY
                     QARYLSGIAAGLKTESNKLGYVAAMGKDNSEVTGGLNAFAMGAYSVNPDAKVYVKVTN
                     SWYSPEEETNAAKALVAEGCDVIAQHCDTPNPQLEAEKAGVFGIGYNSDMSKDAPKAT
                     VTSAVWNWGAYYTWAVEQVVNGTWTSENYFGGMKEGMVDISPINESIAAEGTAEKIEE
                     ARQSIMDGKFNVFDGVIETNDGSTVGEEGKTLDDGTITGGINWYFKTVVEK"
BASE COUNT        10193 a         6538 c         6368 g        10570 t
ORIGIN      
        1 tttcgagtcg agctcgttat gacctcttcg atacgcttcc atgttgatgt caactcttat
       61 attttatcta aagtaatgag cattgtcaac gtgttaatcc ttgaatattt cttttttata
      121 ttttaaacct gcaggggtta tggctactcc tcccagggca gtttccctaa gctccaaggg
      181 catctgcctt cccaccttgt acattgcccg caccacttca tcaaagggaa ttttgctgta
      241 tactccgctc ataacaaggt cggctgttgt aagggcgctt actgtgccgg caatgtttct
      301 tttggcacag ggaacctcaa ccaggcctgc taccggatca catacaaggc ctaatatatt
      361 ttttattact atggctgctg cagataatgc ctgcccggga gtacccccca tcatttcaac
      421 aactgctgct gatgccatgg ctgccgctga gccgcattct gcctggcatc ctccttctgc
      481 accagaaagg gttgcatttt ttgcaattat gattccaact cctgccgcag taaataaggc
      541 attggttaat tcgtcttctg ttttattcag tttttctcct gccgataaaa caaccgcagg
      601 caggattccg caggagcctg ctgtggggca tgctgcaatt ctgcccattg cagcatttac
      661 ttctgaagag gataaggccc tggccattgc cctgttcatg aattcaccgg taagagtatt
      721 tttattgtct gcataatgct taattttata agcatctcca ccgattaatc cgctcactga
      781 atgcacctca ttttcctgtg catatgtgga tgagctcatc ataacatgta aagtggtttt
      841 cattttatcc gtgactgtct gccggctgct ttcgcttttt tctatttcct cctgtatagt
      901 atattcccaa atgggaatgt ttttttgagt gcatatttct attagttttt ttcccgttga
      961 aacaaacatt ataatactcc ctccactatg ggatttataa atttaacatt atcaatttct
     1021 gtgatttttc ttattttgtt cattgcgtct tctgttatta tgctgtctgt ttcaagggtc
     1081 atgtttgcca ttttcccctt ttcgtttcta tatacacgca tgttggcaat attaatattt
     1141 tcttcatata aaatagaaga tatctttgaa attacccccg gtaaatccct gtagcgggtt
     1201 aggagtgtag gacgctctcc tgtaaattca acagcctggc cgtcaatatc aaaaataagt
     1261 atatttccgc cccctattga tgagcctgta atcatggaaa cactgccgtc atcctttgtt
     1321 attataaatc tgactgtatt tggatggaca tcgcccaggt cggcaggggt aaatacaatt
     1381 tctattcctt tttcttctgc tgtttctaat gagtttttta tattttcatc ccagggctcc
     1441 atatctagaa tgccggctgc caatgcttta tccgtgccat gtcccttgta tgtttcagaa
     1501 aacgagccgt ggagcaaaaa ttccactctt ttaattctgc cgcctgctat tgaagcagca
     1561 acctttgcca gtcttgctgc acctgctgta tgagagcttg aaggtcctat cataatagga
     1621 cctattatat cgaaaacact aaaattacgc atattactcc tttttttatt aatgaatgaa
     1681 attcttcatg tttcaaaccc ggtattattt ttattccagt tccttgaatg tttttactgc
     1741 gtgtttcagt ttttctacta ttggcagatt ataggggcat tttttttcac atttacggca
     1801 gcgcacacat tgatccggct taacctccag agtgtcatat ttaagcttgc cgtattcagg
     1861 catcttatat ctgatgacgt agttttcaaa tacaaatacg ttgggtatgt ctattccttc
     1921 gggacagggc ttgcagtaac cgcaccttct gcaaaaatct gttcccaggc tttcaacctc
     1981 tttgtttatt atatcctgct cttcctgtgt taagggctcg gaatctattc ctgcacctgc
     2041 attttcaaca acctgttcaa ttgtatccat acccggtatt gcaactgtta tgaggttcga
     2101 attcataata tatttcaagg atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnncctatat atttttcgct tattgtgtat gcccttgctg tatcaataaa attaatccct
     2281 tgtcttttgc attcaagtat caaattaatt gcttcttctt cgcttacgtg ttgtatggga
     2341 attccgccga atcctattac agacacttcc atgcctgtat ttcccaataa tcgttttttc
     2401 atattctacc tcgcagtttt tgatggatta attatatatt agaaatgtgg taacgtcaat
     2461 tcaaaggaat caaaaaaaat tttgcactct gtgcgtttga cttaccagcc gtttttatga
     2521 aaaaaaccca taaaaatggg gtgagtcata aaagaacatc ccgcaatgtt ccgcgggatg
     2581 tctatataat ttggaagaag ataaaaaagt attatcgata taattatctg cataaattca
     2641 gcatttatac atatattttg agaaatttta ataatattaa ttataaatcg cgggacgaaa
     2701 actggagtac aggtaaacac ccctgttgtc aaatattcta tactgtgata accaattaat
     2761 gaatgtcccc gttacatttt agaaggtgat agcatgaaga aaaacagtct tattattttg
     2821 ccggttttta taatttatat tgcagttttc aggtttattt atctggaaaa tggactgtct
     2881 ttaatatttc aggtgtgttt tcccatattt gcagggatac ttctggctgc agttttaaac
     2941 cctgttttgg ttttttttga aaaaacgctg cagatagaaa acaggtatgc tgctgtaata
     3001 atgacttatc tttttgtttt tctgatgata tctgtagtgg tgaaaactat tacccccaat
     3061 attatagaca gcatcaggca gctgtccctg gatatcccca agctttacag agaagcaaac
     3121 aagctcttgt cgcatcttgg tgatgagttg attgtgaaaa tttatctttc agaaattgca
     3181 caaaagctgt cttccttatt gacagcttta ataaacaatg ctcttacaaa ggtaatcgat
     3241 gtatttatgg catttgccaa tgctttgctg tcaataatca tttctgtcta cctcttaatt
     3301 gataaagaga ttattgaaga ctggattata gatgtgaccg gcatttttgc aggaagaaaa
     3361 acagcaaatg atattgttaa aataggccat atcctttacc gcaatgtttc cagctatata
     3421 tcgggaaaag caactgcttc tttgattatg gcattgttaa tttatacagg ctcaaaatat
     3481 gtaataaaat gtccttatcc tgttatcgac ggaattgtta taggaattac cgatatgatt
     3541 ccttatttcg gcacattcat cggtggtatt cccattgtgc tgataaatgt tttgtatgac
     3601 cccaggaaag ggttcctttt gtttgtttta atattaatat tgcagcaagt cgaaagtctt
     3661 tttatcgacc ctaaaatttt aggcagtcaa ttgtcaataa aacctatatt ggtaattatt
     3721 tcgataataa tcggaggcgg tatgtttggc cctttagggt tgttttttgc ggttcctgtt
     3781 gcagcactga ttaagtcaac agttgatgtt tatatgcaga aaaaaatgga taaaaaggta
     3841 agaattattg ataagccttg aatattgtgc acttttgtgg ataaatctgt tgacaaccgg
     3901 caaaaaaagc ataaaatttg tgtataagta tcacaattaa aatcctctat ttgtttataa
     3961 cttttgcata aaatcaggca gataaaatgt ggaatgataa aaggtaccat acattagatt
     4021 atgaattaaa gaaaatcttt ggcgagaaaa caattaaatt atcaattgac ggaaatttca
     4081 cctgccccaa cagggacgac aatataagct ccagaggctg tattttctgc agtgaaaaag
     4141 gttccggtga tttcactttt tcaggcaaaa caattacaga gcaaattgta gaacaaaaac
     4201 tatttatgtc gaagaaatgg aaaagttcta catatattgc atattttcaa aactattcca
     4261 atacctatga tactgttgac aacttgagga aaaagttcta tgaagcttta agctgtgaaa
     4321 atattaaagg tattgcaatt gctaccagac ctgactgcat atctgaagaa atcgcagact
     4381 tcctggaggg actgaatcat gttacttttt tgtgggtgga attgggactt caatccattc
     4441 acggcaaaac tgcagaattc ataagaagag gttttacctt agaacagttt aacaatgctg
     4501 ttgatatttt aaaaaggaga aatatcagga cagttattca tttaattatt gggctccccg
     4561 gtgaaactcc cgggcaaatg ctgaattctg tcaattatat ttccaatctt gatatatggg
     4621 gtgtaaaaat tcatatgctc catattttaa aaaatacgga tttagctgaa ttttttgagg
     4681 aaaatccttt taaaatattg acccgggaag aatatgttaa tttggtatgt gatgcattgg
     4741 aaatactaaa aaaggaaatt gttgtacaca ggctgaccgg agatggaaag aaatcggatt
     4801 taatagagcc ggcatggact ctcaataagc tcaaggttct ttccgacatt gataaacaat
     4861 tgaaaaacag aggaagtttt caaggcaaaa agttaccatt gtaatgttaa tgtaatacta
     4921 attaaatggt tataatgtat aataatatta agaaaatact atattatgta tagtattttt
     4981 taattgagtg gattaaggat tgatgaaatg aaaaaaacat tattattgat gataatttta
     5041 atatttgctg catcttttac aaatttgtat gctgtcgact ttcctgacgt aaaaagggac
     5101 ttttatttgg aagaggcaat agacgtattg tccggttatg aagttatcag tggatatccc
     5161 gataagactt tcaagcctga cagaatagtc acccgggcag agatggccaa gatggttact
     5221 gtcgcagcgg gattacatga atattccaaa aatatgactt ccgtttatga ggatatgcag
     5281 ggacattggg ctgaaagcta tgtggagctt gcagatgttc tgggtattgt caaagggatt
     5341 actccctcca tttacggccc tgacaacctt ataaagtttg aagaggctta cacaatgata
     5401 ttgaggcttt tgggatatac ggatgaatca ctgattggaa actggccttc taattatcat
     5461 gaaaaggcgg ttgaattgaa tttgtttgaa aatattaacg caaatgttga attcgcatcc
     5521 agaagggata ttacgataat gctgttcaat gctctggata tgaacctggt taccgtaaga
     5581 gaaaataaca ctataaatac tacaaataaa acacttatat ccaagctggg aaagatggaa
     5641 acaagggaag tgtctatcag agacttaaat attgataatt ttgactacac ggattatttg
     5701 ttcaataagt gggatattta ttatgacaac tacggagctg taatccatct gaacaatcct
     5761 caatataacg agtttacggg aacggttaca agtcttcttt caaacagggt catttttgtg
     5821 actgatgatt acggaaatgt gagggttttt caggttcctg atattccgat tgtcataaac
     5881 ggagaaagag gcagctttaa caatttaaat gaatcaaagg ttaaaatagt atatgaggaa
     5941 gattccttca agggtgaagt attgggaatc atcgcctata aggcaactga tgtcatagtt
     6001 gttgaaagag atgatctgta taaggaaggc agcaggcagt ttgccggcaa gcagctgccc
     6061 ttggccggtt cggaaattaa ctataaaaag cttcatattt atggtgatgc cggctccctg
     6121 gaggatataa aagttaatga tgtggtgtat ttttatgaaa caagagacga agagaatcct
     6181 tcattgtctc tgaatgtttt aaggtctcag gttgaaggga ttgttacaag tgttcaaaat
     6241 gcaagagaca atacctttta taccttaaac aatatcagct atatgacagg aaaggattat
     6301 atttttaccg aaaaggcaag tgttaatgat tcggttacat tgatattgga taaaaacaat
     6361 aatattatca agctgaatat caatagttac ggtaaaagac cttcaactta cggtattgta
     6421 ataagctcat caagctctgt caccggctat gcatcggcaa gaatacttga cgaggtcgga
     6481 ggtcttagga cctattacct ggcagacaat tcaaatgtgg tgagagtgca ggaaaatgat
     6541 tcaggtgttt tcaaacaatc attgatagga tcaaatgatt tggtcatgtt tgaccctgtg
     6601 tcaagcggaa cagtaaaaat aataaattta atgccggata gaagcattgc aagctcatac
     6661 aatacccaga cacaaacttt agataacggc ttttatattt catctgatac atttatagtt
     6721 tacagaaaca acggccagta ccagcttttg gttccttctc agcttaacgg atacctggaa
     6781 ggcagggcgg tagtaaatta ttcgggacat atagatgcca tttatctgac taagggtata
     6841 aaagaaactg atgcggtaac agtaacaccg gatacacagc aaaattacaa cggaacaatt
     6901 tacggtgtaa taaagggggt tacaaaagta gacgatacta caagccatgt gcagtttttt
     6961 aacaacagca gcgtattttc cctttcaaac tcatctgctg caggtaaaag tatttcttta
     7021 atgagaaact cctatgtgag ggcggtaatt gtaaacggag tggtttcaag cattgaaaaa
     7081 gtaacagctg aaacatccaa aataaatatt tcacaaattt attcgaatca aatgcttatt
     7141 gataatataa catatatgga gtatgcatct gatttaaagg tgttcatttg cgccttggac
     7201 agttcgggta atataagcag tttcagcgag ggaaatatat cagacatcaa ggccggctca
     7261 acagcccagc tttatgacat ttacggagga tttgacggag ttattgatgt ggtgctgata
     7321 tataaataat gaaaataaat taaggaaggg ggacacacct ttaaagccgg ttggctcttt
     7381 gggagacagc ttttgaagga atgcccctgt actaatgggg acagatgtcc cttttttgtt
     7441 gttaattatt aaaaaacatg ctgccgttca agcccaattg gctttcaccg ccaagcatgg
     7501 aacctaaata aaatatatta ctgcaaattt tatcagaatt attgattata agacatattt
     7561 ttgatataat ttaatcaaat atacaattga gcggcattcc tctgaactaa ccatcaatgc
     7621 caagaggtgt gtactcataa cattatggga ggattaatag atgaacggaa aactaagacc
     7681 tgcctgggtc gaagtaaaca gggaaaaagc tatacataac tttttagagg taagaagagc
     7741 agtaggacca aatgttaaga tatgcgcggt tgttaaagca gacagctatg gaatgggttc
     7801 catggaatta tcaaagatgt atcttgaaaa cggcgtggat atgtttgctg tggctgtaat
     7861 cggtgaagct tttgaactga gagaggaaat aaaagataag gacatccttg tacttggttt
     7921 tacatcagag gaattctacg aagatgccat agaaaacaat attacattga ctgtttacaa
     7981 ttatgaccac gctgtaagat taaaccagac agccaagaaa ttaaacaaga aggcaaaaat
     8041 tcacataaaa gttgaaacag gcatgaacag gttaggattc ttgcctactg aagaaaacgc
     8101 cgataagata gcagatattg caaaaatgga aaatattatg attgaaggtg ccttttcaca
     8161 tcaggccaag gctgatgaaa gggataaaac aactgctcac atgcaggcag gcagatttat
     8221 aaagtttatg ggaatgcttg aagaaagaaa tgttaaaatt cctgtaaagc atattgcaaa
     8281 cagtgcaact ataattgaaa tgcccgagta ttattttgac atggtgagac cgggtattat
     8341 actgtcggga ttttatcctt ctgatgaagt ggatactgaa aaattaaaat tcaaaatctg
     8401 cgttacctta aaggcaaagg ttgctaacgt gaaaacaatc gatgcaggtg aaggtgtagg
     8461 ttatggacac ttgtaccgtg ctgataagcc ggtggtagta ggtacaattc ccataggata
     8521 tgctgatgga tattcaagac tgctgtcaaa caagggatat gtggttataa atggagtaaa
     8581 atgtccgata ctgggaaaag tatgcatgga ccagtttatg gttgatctgt caaatgtgga
     8641 gaacccgcag ataggtgatg tggcaatact ttacggtgac ggtacggatg gagcaatgac
     8701 agcggaggat gtagccaata tgagaggtac catttcctat gaagttctga ctaatttagc
     8761 tgtaaaaaga ctgccaaaaa tctatatata aggagaacta tatgaattat aaaataatag
     8821 aagaaaatga caatgaactg tttcgctata tgatggaaga aaaggaaagg cagagtatta
     8881 atattgagtt gattgcatcg gaaaactttg tatccgatac ggtcctgcag gctatgggaa
     8941 gtcttttgac caataagtat gcagaaggat atcccggcag aaggtattac gggggatgcg
     9001 aatttgttga taaggtggaa aatcttgcaa tagaaagagc taagaaaatt ttcggcgcag
     9061 accatgcaaa tgtccaacct cattcaggat caaatgcaaa ttttggagta tactttgcca
     9121 tactgaagcc gggagataaa atacttggta tggacttatc ccacggaggc catcttactc
     9181 acggaagtcc ggtgaatatg tccggctctt atttcaatgt tgtattttac ggagtgaaca
     9241 aggaaactga aaccattgat tatgaccagg taagagaaat agctaaaagg gaaagaccta
     9301 aacttataat agcaggagca agtgcctatc caagaatcat tgattttaaa gttttcagag
     9361 aaattgccga tgaaaccggt gcttacttta tggttgatat ggcccatata gcaggaatcg
     9421 tagctgcagg cctccatcct aatcctgttg actatgctga ttttgtaaca acaactacac
     9481 ataaaacact gaggggccca agaggcggaa tgattctgtg caggaaagaa ttcgcccaga
     9541 aaatagataa ggccatattc ccgggaaccc agggcggtcc cttgatgcat gttattgcag
     9601 ccaaggctgc atgctttaat gaggttttaa atgatggttt tgttgagtat cagaaacagg
     9661 ttttaaaaaa tgcaaaagcc ctgtcccagg agctgatgaa taaggggttt agaatagtat
     9721 ccggcggtac tgacaatcat ctgatgctgg ttgatgtgag aaacaaaaac ttaacaggca
     9781 aggaagcgga aaaaattctt gatgatattc atatcaccac aaataaaaat actattcctt
     9841 ttgacccgga aagccccttt gttaccagcg gcctgagaat gggaactccc gcagttacaa
     9901 ccaagggaat gaaagaaact gaaatgacgg aactggcaga tattatagat tcagcacttt
     9961 ctaaaaaagc agaagaagac ctgttaagag aaagtgtatt aaaacttaca tccaaattta
    10021 attaagaatg aaagagacaa ggggttaata taattattta ggggtaatga aggcttccat
    10081 gatgtaaacc gttttaaaaa taactgcaaa tgagattaat tgtatgcaat gtgcgcaaag
    10141 gtgtacgcat gctatataaa attgtcttgc aaaatgttgt tttcaatgtt ataatcataa
    10201 aaattttata aaaattagga gagaagtata tgaaaagagt attatctttg ttattgatcg
    10261 tattaatggt gcttacgctg tttactgcct gtactaaaag cgacactaca gaagcagaag
    10321 taatcaaaat tggtgtgttt gaacctctta caggtatgaa cggcggcggc ggacttcaag
    10381 agcttggcgg catcgaatat gccaacaaaa tgtatcctga agtattaggc aaaaaaattg
    10441 aattggttgt agttgacaac aagagtgaca aaggcgaggc aacaaccgca gtaacacgtc
    10501 ttatcgaaaa tgacaaggtt gttgcaataa tcggctcata cggctcaggt gtttctattg
    10561 cagccggaga tattataaaa gatgcacaga ttcctgcaat gggcgcttcc tgtacaaacc
    10621 caatggtaac acagggcaat gatttttact tccgtgcatg cttccttgac cccttccaag
    10681 gcaaggttat ggctacctat gctatagagc agggagcaaa gacagcagct gttataatgc
    10741 agaatggaga tgactactct gtaggtcttg caaacttctt tatagatgca tttaaagctt
    10801 taacgggaaa tgatgcaagc atagttgatg tttcagaatt ccagctgaat gaagctgact
    10861 ttacagcaat tcttacaaat atcaaggcaa aaaatcctga cgtaatattt gcgccaagtg
    10921 cagcaactac agctccattg attattaaac aagcaagaga gcttggaatc gacagcttga
    10981 taatgggcgg cgacacatgg gagaatcctg ctgttataga tgttgcagga gaagatgccg
    11041 agggtatagt tctttcaaca ttctttgatg aaaatgatcc tgctacggaa gaagctaagg
    11101 tattcgtaga aggctacaaa aaagaactgg caggcaaaga gcctataatt cctgcagttg
    11161 cagcattagg atatgatgca tacctgctgg ttagagatgc aatcgaaaga gctggctcaa
    11221 ccgacggagt agcaataaga gatgcaatta aagaaacaaa agattttgaa ggcgtaaccg
    11281 gaattatcaa cttcactgaa gatggagacg ccgacaaaaa tatggctgtt attaagactg
    11341 ttcaagacgg acaattcgtg ttcattgatt cagtagaggt acaataactg atttaaacat
    11401 tcaggggcgg actattcgtt cgcccctttc cagttaattg atttggagga atatatatga
    11461 ctagtgaact tcttttgcag cagcttgcca acggtatttc cttaggaagt ctttatgccc
    11521 tcttggcaat aggttatacg atggtttatg gtattttaag acttattaac tttgcccacg
    11581 gggatatttt tatgatggca tgttatttta tggttttcag catttttact ttcaacctgc
    11641 catggtatat tgcaattata gtagtaataa cagcaacggt tgccctgggt gttcttcttg
    11701 aaaaaacagc ctataagcca ttacggaatg ctccccgtat gtccatcatg atttctgcca
    11761 taggagcgtc ttttctattg gaaaatttgg caacttattt atttacaggt gtccccaggg
    11821 gatttcctga cattaaagtt ttaactgcca cagtgaaatt aggaagtctt tcactgactg
    11881 ttgccacatt gatcacaccc tttgttaccc ttttactttt gtatgttgtg ctcttcataa
    11941 ccaataaaac aaaaatcggt atggcaatga gggcagtttc aaaggatttt gaaactgcca
    12001 agctgatggg aataaatatt aatcaggtaa tatcttcaac ttttataata ggttctgccc
    12061 tagcagcaat aggagcagta ctatggggta caaggtatcc tcaagtaatg ccgcttatgg
    12121 gtgttatgcc gggtcttaaa tgctttatag ctgcggtatt aggcggtatc ggcaatacca
    12181 caggcgcagt tctcggagga tttatactgg gcatggctga aattatgctt atcacatttc
    12241 ttccgtcact taccggttac agagacgcaa tggcatttgt aatattgata gttgtattgt
    12301 tggttaaacc aacgggacta cttggagaga aggtgacaga taaagtatga tggataaaat
    12361 aaataataaa aagaatagat tatttttgaa tataatcatg atagctgtat ttgcagcagt
    12421 aataatacta ttggatacag gaataatagg taatgcctat ataagacgta tattgaattt
    12481 atctgccatt tatgcaatag tgagtgtttc catgaacctg gtcaatggtt ttacgggatt
    12541 attctctttg gggcaggcag gttttatggc aatcggagcc tattcggtag caattttcac
    12601 cgttccgctg gctgaaaggg ctaaaatatt ttatatcacc cttcaaaatc ctgttttggc
    12661 acaaattgaa ctgccttttg ttgctgccct cttgctgggt ggtttattgg cagccctggt
    12721 ggcagtttta ataggcctgc cggttctcag attaaagagc gactatctgg ccatagcaac
    12781 attaggcttt tcggaaataa tccgtattgt ttttacaaac acaaaatcaa ttacaaacgg
    12841 agcactggga ataaaaagca tacctaaaat gcctaccatg tgggttttct tcggtgtggc
    12901 aataatttcc ataggtttaa tgtggctttt aattaactca agctacggga gagcctttaa
    12961 agcaataaga gaagatgaag tggcagcgga agcaatggga ataaatttgt tcaagcataa
    13021 ggttatgtcc tttggcgtgg gagcattttt tgccggaatc ggcggcgggc ttttggctac
    13081 actgctggct acggttgacc ctaaccagtt ttatttcaca gttacctaca acttcctatt
    13141 gataatagta ttaggaggaa tgggaagtgt atccggtact atcatagcat ccttcgtggt
    13201 tacgtcaggt cttgagatac tgaggttctt tgatgagcct ctcacattgt tcggcgcaaa
    13261 tattgcattg ttccgtcccg gattaagaat ggtaatattc tcgattttat tgatgatttt
    13321 ggtgcttttc ttcagaaatg gtcttttggg gcagaaggaa ttgtcctgga agctgatttt
    13381 taactggctt gcaggaaaaa aaccaatcaa aaagaccggt gaaggaggtg ctgcataatg
    13441 agtattctaa aaatcaacaa tgtaactatg aagtttggag gagttactgc tgttaacaac
    13501 cttgatatag aaattcagga aggaaaaatt gaagccttga taggtcccaa cggtgcggga
    13561 aaaacaaccg ccttcaatgt tatcacagga gtttattcgc caacagaagg aaatgtattt
    13621 tataaggatg aaaatataac aggtcttaca cctgacaaaa taacaaaaag aggtattgcc
    13681 cgaacatttc agaatataag attgtttaaa aacctgacgg tcttggataa tatactgatt
    13741 gccaatcatt taagaataaa ttcaaatttt ttgtccagcg cccttagaat gccctggtct
    13801 aacaaggaag agaaaaatat gcgggagaaa tcggaaatgc ttctggataa attgggcttg
    13861 ttgagattaa aaaatgaaat agcccattcc ctcccatacg gtgagcagag aaagctggaa
    13921 atagccagag cccttgcaac tgatgcggac cttctgcttt tggatgagcc ggctgcaggt
    13981 atgaaccctt ctgaaacaat ggagctgtca tcatttattc ataccataag agatgagttc
    14041 aagcttacga tttttatgat tgagcatcat atggacctgg ttatggaaat atcagaaaaa
    14101 atctacgtcc tggacttcgg acagcttata gccaggggaa ccgcagatga ggttaaaaat
    14161 gaccaaagag ttatagaggc ttacttgggg gtggaagaag atgcttaaaa tcaatgatct
    14221 acacgtaaat tacggaggaa tatcagcagt caagggtatc agcatccatg ttccggaaaa
    14281 atccatagta accttaatag gagccaacgg tgcaggaaaa agcacaactc ttaaaactat
    14341 atcaggactt gttaaagcaa ggagcggtga aattttattc caaggtgaaa atattcttgg
    14401 aatggatacc atagatatcg tagcaaaagg tattaccatg gttcccgaag gaagaaaagt
    14461 tttcgtgaat ctttcggttt tagagaacct taaggtaggc gcttatctga gaaaggaagg
    14521 aatcgaagag gatataaaaa gagtttatga attgttcccc atcctgaaag aaagagcatg
    14581 gcagctggcc ggaaccctgt ccggaggcga gcagcagatg ctggcagtag gaagggcttt
    14641 aatggccaag cctaagctta taatgatgga tgagccttcc cttggattag cacccttgat
    14701 agtcaatgcc atatttgaaa ttataaaaga aattaacaag cagggaacaa cgattttgtt
    14761 aatcgaacaa aatgcaaaca aagcccttaa ggtagcagat tacggttatg ttttggaaac
    14821 aggcagcatg gccatggaag gaaagggaat ggagcttctg aagaatgaga aaatcataga
    14881 agcatattta ggaacggcga agaaataaga accggcgttt taaaaggaat atccaaattg
    14941 ggtattcctt tttctgtccc gctatcccat tatcatttgt atcaaaaatc ctgccgatac
    15001 tgaaaaaaca tatatgtagg tcataatttt aatattttct ttaatattac cgttcatttt
    15061 gaacaaaacc agcaaaccgg ctcctgctcc agtggaaaga cctgcaattg cagatccgaa
    15121 gcttaagctt ccactgataa agagctgagt gaggatgaca gatgcggcac aatttggaat
    15181 aaatcctatt atacccgcca atgccggttg aaatatactg tcatttagta aaatacctga
    15241 caggttatcc tcccccacat aggttattag aaggtttaaa aaaaaattag tgactaccag
    15301 aaaaataaat atattgattg tatgtttgaa tgcaggcttc aatatacctt cttcgtcatg
    15361 gcatccgcag tccttgcaga catggcggtg aatagagctt gcaattcctt cactattgct
    15421 tatatggaac aagcctttga atatggaatc tgccgcagct ccagctataa cagccagcac
    15481 tgtttttact gctattaggg acaggattaa ttttatatca ccgttatttg acaataatat
    15541 gggtatcgct tcatctgatg tagatatgaa tacggctatc aatgtccccg cacttataat
    15601 tcttcctgca tataaatttg atgcagctac ggaaaacccg cactgaggca cacagcccag
    15661 gacagctcca ccggcaactc caaactttcg aaggccgatt atgagcctgt ctgatgactt
    15721 atgctcaata tattcaatta taatgtatgc tgcaaacaaa aatggaagca tctttaacga
    15781 gtccattaat gcatctttaa ctaattcctg catgttaacc tcccgctgtc aattatgata
    15841 atccattctg caaatatttg tcaatatatt cattttgttc tgactgattt gttttctatc
    15901 tgtttccgaa aacctgagtt gttgaacagg aatataaatt ctgggcatgc ataaggggat
    15961 attatttttt atataatatc ccctgctaca tatgcttatt tatttactgc ttcttttaat
    16021 gatttacctg ctttgaacac cggtgctttt gatgccggaa tatctattgt ttcgccagga
    16081 tttcttggat ttcttccttg tctagctttt ctgtctctta cttcaaatgt tccaaatcca
    16141 actaattgta cttttccgcc tgctaccagt tcttctttta ttgtttccat aaatccgttc
    16201 agtgcctttt cagcatcttt cttggtgaaa ccggtttttt ctgcaacatt ggaaattaaa
    16261 tcagctttat tcataaaaac cctccttaat aaaaatgtag tttatttttt cattttatct
    16321 aaattcctac tggaactttg attcttccca taactcatcc atttctttca gagtcatatt
    16381 ttttaaatct ttggttgaat ttctttctat gtactcaaaa cgatcaagaa atttattgat
    16441 agttttattc agagcaatat caggattaat cttctcaaat cttgcgaagt ttacaagagc
    16501 aaacaacagg tctccgaact cctcctccat attttcaatg tttccattat tgtattcatc
    16561 tatgaattcc aggagttctt ctttaatttt cattattgct ccctgtgcat caggccagtc
    16621 aaatccaact tccgcagctc ttttttggat tttatagctt ctgcttaagg ttgataatgc
    16681 cttgggaaca ttcctcaggt tatcagtata tgttgtcagg tttttctctt taagcttgga
    16741 gttttcccag cttgttaatg cttcttcaac atcttcagct ttcacatttc caaatacatg
    16801 gggatgcctt gaatacatct ttttacacac attggttaca acatcattaa agttgaaata
    16861 accttcttcg ctgccaagtt cagcatggaa tacgacttga aacaataagt ctccaagttc
    16921 ctcaaccaga ttgtctacat catcattgtc aattgcatca acaacttcat aggcttcttc
    16981 aataacatgc tgtcttattg aattatgcgt ttgttttttg tcccatggac acccgtcagg
    17041 accccttagc aattttacaa ttcttaacag attatttaca ttatacactc tatgttttga
    17101 aatttcaata ggaagaatgc aaaaatatgt tgaaaatccg taattctttt catgatcaag
    17161 caaaaataga ggaatttttt ttacattttc ttccaaaaca tcgatgatga gggaatttac
    17221 atcttcagga tagatttcca ttagttttat tttaagcctt gatgcaatgt ctatatcgta
    17281 aacctgagta ataatattga aggaatcaag gtcacatgaa aattcatctg cttcaagaca
    17341 gtccatgatt ttaatactgt tataatcggc atatcctgaa agttttaagc atttatccag
    17401 aaagctcatt ccatcaatta ttattgtatt tatttcactt ttatattcat ttgtcagctt
    17461 ttttgttact atgtcaccgt aataggggct tcctgcagtg cagtagttga ttttccccca
    17521 ccttcttgac tgctctataa gggtttggat tatgttttcg taaacttcgt caaagttttc
    17581 atacttttca aaataatcat caaaacttac aatatcgata ctttttctta actcattcac
    17641 tattgggtgg cggtctgttc ttgcaaatgc gggtattccc tccgaaaggg catttttggc
    17701 tctcaatgtc aagtcttctt ccgtccctgc ccccaatccg attatttgaa ttgtgttcat
    17761 tttatttcct cacgaatttt tttagttttt ctccttttgg aataagttca aggtcttcct
    17821 ttgtgattgc tcctgtaaca aatagacaga ttacatatac tattccagct gctgctatgg
    17881 agcctagtgt tgccagattc ctgctttctg ctgccgattg tataaaaccg aaggataccc
    17941 atacggcaag agccatgact gcagtggata atagtgctct tgaggatacc ttgatcaaat
    18001 taattctgat ttttgttttt ttgatatcca accagttaaa cagcgctaca aagaaatatg
    18061 caaccgttgt gcttacagct gctccattta tattaatgct tggaattcca atcaaaacat
    18121 aggataaggc taccttgaca accaagccca gggctaagtt tttaacaggg tgaaactgtt
    18181 tgttcacaga ctgcagtatt gctgtaaaag cttgaaccaa agtaagaaaa attactccta
    18241 ttgacagtat ttccaaaagt gccccggaac ttgcatgctt tgcggggcct atcgatggat
    18301 aaaggagggc aattatgggt tctgccagca taaacaaacc tatgccgcag ggaagcccta
    18361 tgatcaatga catctttact cctgcatctg cagtaacact taatttgttc atttgtctcc
    18421 tggtaaaggc atccgtaatt gcagggacta aactcattgc aactgctgtg gagaatacct
    18481 gggggaaatt tatcagggtc tgtgccgtac cgcttaactc gccgaacata tcagcaatct
    18541 gtatatcgtc atatccaatg aatgacagcc ggttggaaac tatatatgaa tccgctatgc
    18601 ccatcaccgg tgctatagaa gctcctattg ttataggaat ggcaatatat aaaagacttt
    18661 ttattacatg ccgtgcagat tctgttttat tgttttttga tgaggcaatt tcttctttca
    18721 tatttttttt gtttaataaa aacattataa atatcagtat aaaggcagca agacctcctg
    18781 ctgatgctcc aaaggtagct cctgccgcag cttcctcaag tcctctgcct accagtgcat
    18841 atgccaggta taagccgaca gcaaccctga ataactgctc aatgagctgt gacaaggcaa
    18901 tgggagtcat gttctgcatt ccttggaaaa accccctgta agcagacagc aagggtacta
    18961 caaacaaggc gggaaccagt gctaccatgg agtagtaaga gccgggaaag ccaagaaatg
    19021 taacaagact tcgggcaaat aaaaatacaa aaacagatga aacaagcccg attaaaaata
    19081 gaccccagcc tgctgttttg taaacctgat atgctccctg acaattgttt aaagctcttc
    19141 tttcggaaac catcttggca atagctgtgg gaaatcctga cagggacact acaagcaaaa
    19201 gattatatat attataggca ggctgatagt agcctatccc ctcggtacct attaaatttg
    19261 tcagaggtat tctgtatatt gctcccaata tttttacaaa taatcccgac acgcttagta
    19321 tagcagcgcc tttaataaaa ttctccttag acatgtttct ccctttctta ttatcattgg
    19381 ggacattccg aaatgccccc gtacaattaa gtatcaaact gaaattcagt ttaccacagt
    19441 ttttctttat tttaaagaga aatttgtaat attccaaata aatgatatct accataaggg
    19501 tatatttgtg gtataatttt gctatgacta tctgggacat tcaagtatag ggtgtaaagg
    19561 agcattaaat ggaaagtctt aaaggcacaa ttgttgaaac tatttttaga aataatgaaa
    19621 acggatacac cgtggcttta ctggaaacaa gtgatgaagt aataacactg acaggtatct
    19681 tcagcactga tgtttccggt gaaaatattg aagtttttgg agaaagagtt aatcatccaa
    19741 aatacggaga gcagtttcaa gtagacatat acaattccat ccttcccaca ggtctggaac
    19801 agatagaaag ctatctgtcg tcaggtttga taaagggtat aggcaagtat acggcaaaaa
    19861 aaatagttga cgtttttaaa gaggatacct ttgacattat tcagaacaat cccgggaggc
    19921 tgactgaaat tgacggtata ggcggtaaaa aggcagacat gatcgcgaga agctttcagg
    19981 aacaagctga tgtaaaagaa gtcatgatgt ttttgcaaag ccacaatatc agcaccagct
    20041 acagcatgaa aatctttaaa gaatacggta atagcacaat aaatgtaatt aaagataatc
    20101 cctaccggct ggctgaagat atttacggca taggttttaa gcttgcagat aaaatagctt
    20161 cctctctggg ggttgagaaa gattctcctt acagaatatc atcgggagtg aaatacgttt
    20221 tggcggaata tgcagcaagg ggtcactcct atgtgccgta tggcctcctg gtaaaaaact
    20281 cttcagacct tcttgaatgc tcaattgaaa atacggaaga tgaaataagg aacatggctt
    20341 tcacagataa aatacatatt gaaacaatta attccgagtc tgtggtatat tatcttccct
    20401 atcacactgc tgaagtaaat gtatgcaaaa atttaataaa tttatcccgg ccggacctaa
    20461 gcattacaga ggaagatgtg cttatgcgcc tggaatctca tgaagacagg tctatatccc
    20521 tgactgatat gcagaaggaa gcagtggtcc aatctgtatt aaacggtgct acggtcataa
    20581 caggaggacc gggaacagga aaaacaacaa taattttatc aattattaaa atatttgagg
    20641 ggatgaataa aaaggtcctc ctatgcgctc caaccggcag agcagccaaa cgtataaccg
    20701 aatccacggg aagagaggca aaaaccatcc accgcctcct ggaatatgcc tacggggaag
    20761 atgacagcaa tttgaccttt aataaaaatg aaaactcccc cctggactgc catgcaatta
    20821 tagttgatga aatgtccatg gttgacatac tgcttatgaa taatctgctg aaagccctga
    20881 aaagaggcac aaagcttata atggtcggag atgttgacca gctgccctct gtaggtgcgg
    20941 gcaatgtttt atccgacata attaagagca acaccataaa gacaatccgc cttgacatga
    21001 ttttcagaca gtctctggga agtatgattg ttcaaaacgc acatgctgta aacaaaggtg
    21061 taatgcctat attgaataaa aaggattcag atttttattt tatgagagaa agcaatataa
    21121 ataatatcgt tgacctgata gtcgacttgt ataaaaaccg ccttccttca aagtacggtt
    21181 ttgacccaat gaaagatata caggttttat cgcccatgaa aaagggagat gcaggagtta
    21241 ttgaattaaa taaaaaaatt cagtcggcag taaatcctcc ttccaggctg aagaatgaaa
    21301 aagtattcgg caggtacacc ttccggacag gagacaaggt aatgcaggta aaaaacaact
    21361 accaggcaga atggaaatta aaagatgatg attttattac cggagaaggt atatttaacg
    21421 gtgatatggg aaacatcctc tttattgata atgtggaaca agaaatgcat gttttatttg
    21481 atgatgaaaa ggaagtaata tattccttca gccaattgga tgaattgata cttgcctatg
    21541 ccacaacagt gcataaaagc cagggaagcg aatttcccgt tatcataatg cccatggtat
    21601 ggggaccgcc catgctcctt acccgcaatc tgttctatac tgcaataaca agggcaaaga
    21661 ggctggtagt tcttgcagga caggaaaaat acatggaaga aatgattgaa aacaacaaaa
    21721 tagacaagcg tttctctgca cttgactaca ggctgaggac agtgggacag tgggaacttg
    21781 cctaaggttt ctcgctgaaa cataagatac atctttggaa caatgggggg ataaaatgaa
    21841 agagtatgct gcatccttct tagaactttt atatcctgaa aaaaacacct gctttatatg
    21901 caatctttat gatgaatcca tcaatgacag gtatatttgc agtgactgcc aaaaaaaact
    21961 caaaaaaatt atgcctcccc tgtgcattaa gtgttccaag ccaattgact atgcatctga
    22021 atgtctgtgc ccggactgtg ctgtatatga gaagagcttt gaaactgcca aagccccttt
    22081 ttcctatgaa ggtattgtaa aggacagcat ctacagctat aagtattaca ataaacctta
    22141 cttttacaag tttttcgccg cctgccttct aaattatatg aaagaaaatg actacaccag
    22201 ctttgactac ataacatcag taccccttca caggtctaaa atgcgcacaa gaggctacaa
    22261 tcagtctgaa ctgctggcaa aacacctgag ccgtgaatta tccattcctt atgctgatgc
    22321 cttgaaaagg accgtaaaaa ctccaaaaca aagccagcag agcaaggatg aaagaaggaa
    22381 aaatctgaag ggtgcatttg ctgtaaaatt tcctcttaga aacgaaaata taaaaaacag
    22441 cagagtcctt ctggtagatg atgtctacac aacagggtcc actgccgatg aatgctctaa
    22501 agtccttctt gatttcggtg tttcaaaagt ctatgtgatt actatagcca ggtaagtaca
    22561 ggggtattct ttctgctggc aagactatca aagagaagat gtatcccttt tcattatgct
    22621 tttttccgtt attatcatgt ccattttaac atcgtgttcc tctgctggta tttcttcaag
    22681 tacctggaaa tcatgggcaa cagcaacctt ccttgctgat gctttcaact tgtttagaat
    22741 cctgtcataa taccccttgc cgaagccgat tctgtttaaa ttcttgtcaa atacaagacc
    22801 cggaattata accagatcga tttcttctat attcactgga gaaacctttt tcggttcact
    22861 gtatccgaaa gaattcagct ttaagtctga ttccccggtt atttcagaag gaatcaagac
    22921 cgtacttact gtatcggtgt atggtactac aacacgtttt ttctcaaaca gcatatgttt
    22981 gataagatcc gatgtcataa cctcattctt gaaatccatg tatatgaaaa tgaccttgct
    23041 ttgtttgtaa acatcaagac caataatttt atcccttatt atgcggctgt tatttaccac
    23101 atcttctccg ggcatattgt ttcttatact tagaattctt tttcttaatt cctttttaga
    23161 ctccattttt acctcttaaa aaaatattga aaataaaaat ctgtatataa cattaatgac
    23221 aggtcctatt attcttccta ttcccctgaa tacaatcagg aggagaagtg caaacctcag
    23281 ttgttcgcga tacttataat aaaaattcca aacctttgct cctcctatgc ttcccaatat
    23341 gtggtgacca tccaagggag gtatgggtat aaggttgaat gccataagaa ccagattaat
    23401 atataccgtg cttctcaata cttcaaatac tatttgattt ggcagagtgt aaggtaaata
    23461 catatctgcc agtttcataa gccccataaa taaaaatgca actaaaaaat tagataccgg
    23521 tcctgccaat gaaacaagag catcatcccg tcggggattc ttaaaattcc tgggattaat
    23581 ctgcaccggc ttagcccagc cgaagccaaa gaccagcagg cataaaaagc ccataggatc
    23641 caagtgactt aacgggttta aagttatacg cccctgcata agaggtgtat catcacccat
    23701 tctgactgct gcataaccgt gggagcattc atgaacagat atacctagta atatacccgg
    23761 cagatacaac aatcttccta ataaaaaatc cataaaaatc ctccttaaca gtatacaaat
    23821 aatttttctc tttattcttc accgttgtcc aaattcttgt atactattga tgtttcaagc
    23881 cttctgcctt ttatttccgt taccaggatt ctaagtccat attcctccag ttccaaagtg
    23941 cttccatcct caggaataat tcccaatatt ccgaaaacca gccccccgaa tgtatcgaaa
    24001 tcttcatcgg gcagactcac ttttaattcc ctggacacat cgtccagata tgctgaccct
    24061 tttatcttcc aggtattgtc atctattttt tcaatttcct cttctccggg tatcaggtaa
    24121 tcatcgtcaa tgtcccctac tatctgctcc agcaaatcat tcatggtaat aataccgctc
    24181 attcctccgt attcgtcaaa aacaactgca aaatggtttc ttgtcttttt catattttgg
    24241 aacaaaacat ctgtcctcac tgtctcagga acaaaatatg caggcttaac aacatttttc
    24301 ataatatttt ctctgctctt atcttcaatt ctgaaatagt ccttgacata cagaactccg
    24361 ataacattat ctgcacttcc gtcacatacg gggtattttg aatgtctgct ttgggatatt
    24421 ttatcgtccc attcatcttc tgtttcatca agccaaagaa ttactgcttc tgttctgtga
    24481 gtcattactt cttcggctgt tttattatca aattcaaaaa cattttgaat catttctttt
    24541 tcattttcat ttatggaccc tttttcgctt cccacatcta ccatcatgcg gatatcttct
    24601 tccgttacct catcatctac agcatcagga tcaataccta taagccttaa gacactgttt
    24661 gttgatacgg tcaaaagcca aaccagggga gcaaacagtc gtgctataaa atataaaagt
    24721 cccgataagg acaaggctaa tctttctgca tttttcattg ccactctctt aggaacaagc
    24781 tcccccatca ccagtgttat ataagccaat aaaactgtga ttaatactac cgatacggga
    24841 tttaaaactg aaaatggtat gttaaggcct atttttatca aaagattggt aagcctgcct
    24901 gcaaagtttt ctgcagcaaa agcgcctcct aaaagtgatg ccagtgtaat ggcgatttgt
    24961 attatggaaa gaaattgtga tgaatcctgt gtaagctttg taagtcttac ggcacgctta
    25021 tccccctcca gcgtaagctt tgctattctg ttgtcattca ttgaaataac tgcaatttca
    25081 gctgatgcaa aaacagcgtt aacaaataac aaaatggttt gtaaaatcag cggaccccat
    25141 atagagcctt catctaacaa agtattaaac ctcctaaaat agataattac aagaataata
    25201 acatagatag tctacccagt caatattcat ttattaggga gaactttata aaatccatgc
    25261 aaattgcata tactgtaaca ggaataattt aattttaacc ttaaagaata gcattttttg
    25321 tcaaatataa gttgctataa ttcaatatat attgtaaaat tattatgatt ctatgtaccg
    25381 gaggcaggaa atgtttctcg aagcgttagt attaggaata ataataggct ttataagaag
    25441 aggaaagctg agcagattat catatgttaa tttctctttc agacccttga tttacatttc
    25501 agctttgttt tacctgggca ttattgttat aaatttaggg ctttatgact atgattcatt
    25561 tttatattca gcatttctta ttgggtctat ggtttttacc ggattgtttt taatagcgaa
    25621 tatacatata aggtttatgt ttattccgct ggttggactt gctttgaatc tgctgtcatt
    25681 tttggcaaat acattcaaat ttcccctgtc acctgaagct acggcacaaa tatacggtca
    25741 ggaagcaaca gaactgttaa acgcaggcaa attattattt tatacatctt cggaaacagc
    25801 agtgctgagt tttttaggca gtattattcc cgtaggaaac tggttcgtgg taagcatagg
    25861 tgatataata gccgccttgg gagtagtttt gattgttcag tgcattatgg cagataagtt
    25921 catacaaaac agagggaaaa ttaccttttc aaaggatata ttcaggtgaa taagtgttaa
    25981 aaaagcgcca tttaggcgca tttttgtttt attcacttaa tcaggattct tttatagttt
    26041 ctgtcaatac ttttgaaagt cctgctaatc cctgaatttc cgatggtact attatctttg
    26101 ttgcctgacc gttggctgct ttttcaaagg attcaaaact cttgaatgtg agtacttcct
    26161 tgttaagctc aacatctttt atcatcttca agccctcagc ggttgctttt tgaatgctta
    26221 aaattgcttc tgcctcgcct tccgcctctc tgattgaagc ttcttttttt gcttctgccc
    26281 ttaaaattgc tgcctgtttt tctgcctctg cttcaagtat caaagattcc ttcttgccct
    26341 gggatactaa tatggcggaa cttttttgtc cttcagcaat gagtatggcn nnnnnnnnnn
    26401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aaaacttcgg gtcggtaatc tggaaaaata
    26521 ttaccgtatc tatctgcatg gttacattat ccttggttat aacaggctgg ggcggaaaat
    26581 caattacctg ttccttaagc gatactttct tgaccaccct gtcgatcaag ggaaccttta
    26641 catgtacccc cacgctccat gttgcctgat aggctccaag acgctctata acataggcat
    26701 ttgcctgagg cactattctg atatttgctg cgagcagtac aacaactatt gcaagcaata
    26761 aaacaaaacc taaatattct aacatttaat tcctccttat tttttttcaa ccataagctt
    26821 gacgcctaca atacttctta ttatcacaat ttcatccttt tcaatcacct ggtcatcaac
    26881 agaccttgca gaccacatct gtccttttac atctatcgtg ccggtattat taatattgtc
    26941 aattgtcgat tgaaccttat aggactgccc tatcaatgat tcagcattgg tcctgactct
    27001 gccgacctta aacttttcca ttgcaatcgg ccttgtaaat ataagacata ctatagaaac
    27061 cagcaaaaat acaataattt gtacttccat tccaaaaccg atcaaataaa ccaaccatgc
    27121 aacaattgct ccggtagcaa accatattgt tgtcaagctg actgtcatag cttctattat
    27181 tacacatgca acaactaatc caagccaggc gtatcctata tattccatcg tgacctcctt
    27241 atactctata ttgattatat accataaaat catacatcct aaacagtttt ttagtcaatt
    27301 ataccgctta aggtctgata tggatttaca tacgtattgt atatgcttac tgcataatgc
    27361 aggtgaggac ctgtcgaaaa tcccgtggta cctacagttc cgatagtatc tcccttttta
    27421 acctggtctc ccttctccac cccgatagta tttaaatgat aataggaagt aaaaagcccc
    27481 attccatggt caataacaat ggtgttcccc ggagaaagaa ggccttccat ggcaaaggtc
    27541 actcttccgt tatttggtgc tatgacagga gtacccttgg gagcagccaa atcaagtcct
    27601 gaatgcctgg atgcagaggg ctcattgttt acatatctta tttcagcaaa atctgttgtc
    27661 agctcacctt caaccggcat tacaaacgtg ccctcccaca gcttttcttt tgcagaatct
    27721 gttcttgcgg gcttcacaag ctgggcaaat tcataaattg cagtattatc attgttagtt
    27781 tcattcattt cctcgctgac ggtaagatac tgggttttga aacttttatt ccctacagtt
    27841 aatgtttttt ccagcatata ttctctttca ctgccttcat ttactacagc cgtaacaagg
    27901 tagtccccgg gctgtgcata caggtctatg gggaaaatat aatagttttt atccttataa
    27961 acataaatat cattgtcaat ttttacggca tctgtatata gctttatcgc ctcatcagta
    28021 tttaggtttt cgacagaaat cagcaaaata ttgccgggat agttttcttt tgtaactatg
    28081 ttaatgtctg caggtatgtc aagcagggca ttaaatttta gtgtcctgct gccatatgat
    28141 ggggagtctt ccttatagcg ccacctgctt tcacattcaa ccaggtattc accgtcgctt
    28201 tttatgctac ttaaaacctc ccccagctcc ttccctgtat ataataagct tttgtcctgg
    28261 tcataaattc ttattgtttg gttatccggc tgcttttcat gtttgatatc tataacatcg
    28321 gtttgtgaca gggataccag attattgttt tcaacttttc cctttagtat cccctggttg
    28381 ttcacgaaat gtccttcaat atttttgctg ttccagttat attccacatc cggacttagt
    28441 ttttcaccgt tcagctcaag atatgaatca taaatttcag aaacatatat atatgaaaag
    28501 acttggctta aaaacaactg tctggcggca ttgtccttta ttttataaat tgtgtcatcc
    28561 tttgcaatat acgcggcctg gctgcctgta tcaaaataaa ttttgtaaat ttcccagctc
    28621 ttgtcttttt tgagtatgat ttcataattt aacaaggttg atatatcctc ttctgttttg
    28681 tccttcttat ttaccgcatc caaaattatg tcagcatcct tgctttcttt cggaattaaa
    28741 gtattatcac cgttttgcaa atttactgct tcgatttcag taacatcaga aaaaacagtg
    28801 ctgcagccgg ttaataatac agacaatatg acaataaata ttattttttt cataatacct
    28861 ccatttcaag tagtttttga ttttgaaaaa gtattattca taattatata tattattata
    28921 tggaaatgag actgttaaaa ttcacaataa cagtataaca tacagggtgc tgattgttaa
    28981 atagatagaa tttggatttt atttaaaaag agctgtcaaa ttgatatgac agcccaaatt
    29041 tcgtattgag attccaaagt ttatctttct tccctgaaat aatttactcc gcagcttgca
    29101 ggctgtgagt tttcctttga ttttcttatt gaagtaataa ccagtaccag ggcagtaatg
    29161 ataaacggaa gcatatcata aaatgcattt ggaaaagtaa aaattgtttt tggaaaataa
    29221 tattttaaaa tattgaaggc tccgaatata aatgagccga aaattgccct taaaggcctc
    29281 catgatgcaa atataacaag agccacggca atccatccct ggccgtttac cgggttgttc
    29341 atccatacgc cgccattgat aatcattgaa cagtatgccc ctcctatgcc gcatatgcct
    29401 cctcctatga ggacatttat atattttagt tttgttacct ttatgcctgc cgcatcggct
    29461 gctcccgggt tttctcctat tgctctcaca ttgagacctg tctttgtgcg gctgtagtat
    29521 atgccgcaga ttactgcaat aaacagtcct aaataaacaa agatgttata ttggaaaaac
    29581 aagggtccta taaccggaat tgtgcttagg cctggaatat ttaaattgct tatctgggaa
    29641 gctatctgct caggaagctt caatgaagat gaagcagaat ttaccagcat aaattctccc
    29701 gcgaagtttg atattcctac accgaagatt gtaagtgtca gtccggtaac attttgattt
    29761 gccataaatg taattgttaa aacagcataa atcaaagcac caagcacgcc tgccaaaaaa
    29821 gctgccaaaa gagcaattaa tatgctgtca taaaaatatc cggccataaa gcctgcaaca
    29881 gcacccatag ccatcattcc ttcaacccca aggttcagga gtcctgctct ttcattcatt
    29941 atttccccta ctgtgccaaa aatcagaggt gttcctgcga atacagctgc aatcaagaat
    30001 tttattagca ttttatctac ctcctttttt ggtcagtaca aacttatatc taatgaaaaa
    30061 ttcgcatcct aaaataaaaa acaatattat cccctgaagg acgccggcaa agtatgttga
    30121 cagcccatag gtggattgca tgacgctgct gcccttttca aggacactga acaggaagga
    30181 aaccaaaagt attccgaaag gattcaaatt cgctagccag gacactatta tggctgtaaa
    30241 acccacacct cctgcaacgg aagacgccaa tgtcatgtct gagcctgcag cctgaatcat
    30301 tccagtaata ccgcagatgc cgccgcttat aatcattgtt cgaagtatta tctttctaac
    30361 atccattcct gcatatctgg cagtggattt gctttcacct acaacgctta tttcataacc
    30421 gtgttttgta tactttagat aaatatatac aagaaacacc aaaatcaggg cgataatcca
    30481 tccgaaatga actcccagca ctttatctat ttgagcattg gcggtaaatc ttgcaatttt
    30541 tgggaaccct gaagatcccg ggtccttcca gggaccatcc cgtaggtatg ttataacgta
    30601 cagggctata taattgagca tcaatgtaaa aagtgtttca ttggtgtcat attttgattt
    30661 gaagtatgca ggtattaaac cccacagacc tcctcctatg gctcctgcta aaaacattac
    30721 aatcaataat aaaaagtgag gccagttatc atgataaagt gcaaagtatg aagcgcagac
    30781 agcccctgca attatctgtc cttcagctcc aatattccaa aacctcattt taaatgccag
    30841 agccacgccc aaggatgaca tgagcaaggg aatcataaac cttattgttg cctgggctgc
    30901 cattttgctc cggaatgctc cggatatgat ggtcccgtac aattccagcg gattatagcc
    30961 caggagcagt atgaaaatac ctcctgccgt aagtgccgac acaaaggcaa ttattcttat
    31021 atttatggta tccttgtttg aacgttcgga tatttttaca atcctgataa aaggagtgtg
    31081 gcttttgtta ttcatctgag tcaactcctt tcatcttttt tcctgccatc attagcccta
    31141 tttcttcctt ggcagtattt tttgcatcca cgattcccat tatttcaccg tggcataaaa
    31201 ccataattct gtcggacagc tctaacagaa catccaaatc ttcccctata aatagtattg
    31261 ccacgttttt tttcttttgt gcattcaata aatcataaat tatataggaa gaatttatat
    31321 caagacctct aaccggatat gcggtaatga gtacatttgg ttctgattca atttctcttc
    31381 ccaaaagaac cttttgaaca tttcccccgg acagcattct tacgggagtt tcaacgccag
    31441 gtgttacaat gctgagttta tcaaccagtt cttcagctaa gagcctggat ggtttcttat
    31501 cgattataaa gcctttgttt tttttgtatg atttaagaag catattgtct atcatgtcca
    31561 tggagcctac aagccccatt cccaatctgt cctccggcac aaaactcatg cttatgccta
    31621 aatttataat ttcatcggga gttttcccta taatattttc tttgttatat aagacggcgc
    31681 ctttcctggc atccataagc cctgcgattg tttcgcacag ctccctttgt ccgctgcctg
    31741 caattcctgc tactcccaat atttctccgc ttttaagggt aaaattaata tccttcaagc
    31801 ctattgtacc atcctcattt acaacagaaa gatctaccac ccttaaaaca gttttattgt
    31861 tctctgtttc aggacgtttt atttcaaggc tgatgggtct gccaaccatt aactctgtaa
    31921 gctctttttc attggtgtcc gcggttttta cagtatcaac cacctgccct tttcgaagta
    31981 ttgccaccct gtcgctgatg gctaaaactt cgttgagctt atgagtaata ataattatgg
    32041 catttccctg ctccttcatt ttacgcagta ttataaacag tttttccgtt tcctgaggtg
    32101 tgaggactga agtgggctcg tctaatatta atatttttgc ccccctgtag agtaccttca
    32161 ttatttcaac agtttgtttt tcgcttacgg acatattgta aacttttttg tcaggctcta
    32221 tttctaaacc gaatttattg ctcatttgga gaatttcttc ttttaatgca ctgcttttta
    32281 catatttacc ttttgtgccc aaaacaatat tgtccattgc actgaacact tccaccagtt
    32341 taaaatgctg gtgaatcatt cctatgcctg aatttattgc ttcatttgga tttgttatga
    32401 cagcttcttt gccatcaatg gatattgtgc cttcatctgc ataataaatg ccggataaaa
    32461 tattcatcaa tgtggtcttt cctgacccgt tttctccaag caaagcaaga atttcaccgt
    32521 atttgatgcc aaggcttaca ttgtcgtttg caattacctt cccgaaggtt tttgttatgc
    32581 cctttagctc aattgcataa tttgtctcat ttgttttcaa cataatctcc tgtttatgtc
    32641 attaaggggc tggtaagaac caaccccttt aattattatt tttccactac tgttttaaag
    32701 taccagttga ttccccctgt aattgtcccg tcatccagtg ttttaccttc ctctcccacc
    32761 gtagacccgt cattggtctc tattactccg tcaaaaacat taaatttgcc gtccataata
    32821 gactgtcttg cttcttctat cttttcagcg gttccttctg cggcaatgct ttcattaata
    32881 ggtgaaatat caaccatgcc ttccttcatt ccgccgaagt aattttcact ggtccatgtt
    32941 ccgtttacaa cctgttctac agcccatgta taataagctc cccaattcca tactgctgat
    33001 gtaacggtag cctttggagc atccttggac atatctgaat tatagcctat gccgaaaacc
    33061 cctgccttct ctgcttccag ctgagggttg ggagtatcgc aatgttgtgc tattacgtca
    33121 cagccttcgg caaccagcgc ctttgctgca tttgtttctt cttccggtga ataccagctg
    33181 tttgtaacct ttacatagac ttttgcatcc ggattaacgg aatatgctcc cattgcaaat
    33241 gcattcagtc ctccggttac ctctgaatta tccttgccca ttgcagccac gtatccaagc
    33301 ttattgcttt ctgtttttag tcctgctgca attcctgaca agtatcttgc ctgatagatt
    33361 cttccaaagt agttgttaaa gttcctgtca ttgcctttat accctgaacc gtgtgaaaat
    33421 attacatcag gatattctgc tgccaatgct tccattgtat ccatatatcc ccagcttgta
    33481 ccgaaaacga tgtgagctcc ttcttcaata cattcaagaa ttgcagcttc agtagctgta
    33541 gggtctgtat catttacatt gttcttgcgg attatctggc tgtcatctag acctagtgca
    33601 ttctgcattg ctactattcc ctggtcgtgt gcaaatgaat aacctgaacc atctgcagga
    33661 ttgcctatat gaattactcc aaccttaata tcttccttgg gaactgcagg gaaaactcct
    33721 tctcccgaag gtcctggctc ctggggtcca ggctgctgct cattgctgcc acatgcagtc
    33781 agcattgtaa gtgccagcac cagcactaaa atgatgctta ataatttttt cattaacatc
    33841 ctcctaattt tataaacttt gcaccaaga
//
LOCUS       DAOPUT010000010        33867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_1218_length_33867_cov_19.778747, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000010 DAOPUT010000000
VERSION     DAOPUT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 33867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33867
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1218_length_33867_cov_19.778747"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="ylqF"
                     /locus_tag="PKK75_02045"
     CDS             complement(<1..777)
                     /gene="ylqF"
                     /locus_tag="PKK75_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNZ82408.1"
                     /translation="MNINWYPGHMKKTKELLQENIKLVNLIIEVVDARIPYSSKNPDF
                     DKLFRDKKRLIVLNKYDLSDPVMNKEWEDYYKSRGWSVVLYNSTNNKELDRLNSAIAE
                     ATREIIERYKKKGFVKKTIKAMIVGIPNVGKSTLINSLAKKKSTRTGNMPGVTKGKQW
                     IHLAGNIDLLDTPGILWPKFDDEEYALNLAFTGAIRDEVMVLEEIALKFVEKMQQMGK
                     ENSILERYGVEAGEKPLDILDNIAYKKGFLINKTQIDYLRA"
     gene            complement(953..1297)
                     /gene="rplS"
                     /locus_tag="PKK75_02050"
     CDS             complement(953..1297)
                     /gene="rplS"
                     /locus_tag="PKK75_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNZ82409.1"
                     /translation="MDLIKTIENEQIRSVTAFNVGDTIKVHVRIKEGNRERIQVFEGI
                     VIKIQGESSRATFTVRKISYGVGVERTFPVHSPKIEKIEVVRKGKVRRARLFYLRERT
                     GKATKVKELRKY"
     gene            complement(1397..2113)
                     /gene="trmD"
                     /locus_tag="PKK75_02055"
     CDS             complement(1397..2113)
                     /gene="trmD"
                     /locus_tag="PKK75_02055"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNZ82410.1"
                     /translation="MKFEIITLFPELIEVFKNNGIIGRAVENKLFELEYVYLRDYSED
                     KHKKVDDYPYGGGPGMLLRPEPMFKALDAIKRHNSYIVYLSPKGSLFNQNKAKELSVM
                     EHIILIPGHYEGIDQRIIDKYVDEEISVGDYVLTSGELPALMIVDAVGRLLKGVLGSE
                     ESSREESHSENLLEYPQYTRPESYMGLDVPEVLLSGHHKNIEEWRLFKAIEITVKRRP
                     DMIKDESIREKYKALSKKYK"
     gene            complement(2110..2610)
                     /gene="rimM"
                     /locus_tag="PKK75_02060"
     CDS             complement(2110..2610)
                     /gene="rimM"
                     /locus_tag="PKK75_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227124.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNZ82411.1"
                     /translation="MKDYIKVGKIINTHGVRGYVKCMPMTDEPERFEELEYVYTEKDS
                     IKRIIHHVWYQKGMVYLKLWGIDDMETAETFKDTYISILDDQLRKLPDDSYYLFDLDG
                     MDVYSSTGEYLGKISEIYQTGANDVYEVKNKERSFLIPAVKDVVKSVSVKDKKMIIDV
                     IEGLLE"
     gene            complement(2625..2855)
                     /locus_tag="PKK75_02065"
     CDS             complement(2625..2855)
                     /locus_tag="PKK75_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNZ82412.1"
                     /translation="MGELVEYIAKSLVDNPDMVEVNEVEGSQSLIIELKVAPEDMGKV
                     IGKQGRIAKAIRTVVKAAATKDNKRVIVEIIQ"
     gene            complement(2867..3130)
                     /gene="rpsP"
                     /locus_tag="PKK75_02070"
     CDS             complement(2867..3130)
                     /gene="rpsP"
                     /locus_tag="PKK75_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNZ82413.1"
                     /translation="MSVKIRLKRMGSKKKPFYRIVVADSRSPRDGRFIEEIGYYNPVS
                     EPKVVKINDESAIKWLNNGAKPTDTVNMLFKNNGIYEKRDENK"
     gene            complement(3294..4643)
                     /gene="ffh"
                     /locus_tag="PKK75_02075"
     CDS             complement(3294..4643)
                     /gene="ffh"
                     /locus_tag="PKK75_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNZ82414.1"
                     /translation="MFENLSDKLQSALQKLRGKGKLTEKDIDLAMKEIKMSLLEADVN
                     FKVVKSFINNVKERSLGAGVMESLTPGQQVVKIVHEELARIMGQGESKLKFNSNNITY
                     FMMCGLQGAGKTTATGKLARKLKKDGRRPLLAACDVYRPAAIKQLKVVGESVDVPVFE
                     MGDKISPVTIAREAMNHAMKHGNDVVIIDTAGRLHIDDDLMDELVKIKETVKPDEILL
                     LLDAMTGQDAVKVAETFNSYLDITGVILTKVDGDARGGAALSIRNVVDKPIKFLSVGE
                     KMDQLETFHPDRMASRILGMGDVLSIIEKAQNAFDEKQAVELEKKLKTQSFNLEDFLA
                     QLQQMKNMGPLDEILEMVPGLGGNKKLKGLKVDEKELVYTEAIIQSMTKKERLNPSII
                     NGSRRKRIAAGSGTSVQKVNSLLMQYEEMKKMMKKFSSMGTGRKKGFGGLGGFKLPF"
     gene            complement(4646..4993)
                     /locus_tag="PKK75_02080"
     CDS             complement(4646..4993)
                     /locus_tag="PKK75_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNZ82415.1"
                     /translation="MIEKCFIYSILYDYYGQLLKENQAAIIEMYYNLDYSLSEIADEL
                     NISRQGVYDALKRAEKNLSDYEKKIKLHDKYVKFSDAAENIIKLSSMIDEDKYKDIIE
                     KIKTEAAKIINEG"
     gene            complement(5372..6289)
                     /gene="ftsY"
                     /locus_tag="PKK75_02085"
     CDS             complement(5372..6289)
                     /gene="ftsY"
                     /locus_tag="PKK75_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNZ82416.1"
                     /translation="MNEKKGFFAKLKEGLTKTKKNLTEQIESVIFQHKRIDDDFLDEL
                     EEILITSDMGVETTMDIIEYIKEETKKRKLEDTLEIKDVIKEYLINTLKENERPDTTD
                     KQRVILIVGVNGVGKTTSIGKIAYRLKQAGNKVIVAAADTFRAAAVDQLKEWCQRAGV
                     DIIASDYGSDPGSVVFDAIQATKARKADILICDTAGRLHNKKNLMNELSKIFKILDKE
                     FSEAHIDVYLVIDATTGQNGIAQAKVFKEACNINGLILTKLDGTAKGGIAFPIVNELK
                     IPIKYVGVGEQIDDLQDFDAQDFINAIFE"
     gene            complement(6378..9938)
                     /gene="smc"
                     /locus_tag="PKK75_02090"
     CDS             complement(6378..9938)
                     /gene="smc"
                     /locus_tag="PKK75_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNZ82417.1"
                     /translation="MYLKKIYVNGFKSFAEKTEIDVEKGITAVVGPNGSGKSNISDAI
                     KWVLGEQSAKTLRGGKMDDIIFAGTEKRLPLGYAEVRLTFDNEDGTIPIEYKEVSISR
                     KLYKTGESEYSINKQQCRLKDIRELFMDTGIGREGYSFIGQGRVEDILSPNSDVRRQV
                     FEEASGIVKYKSRKEESEKKLEKTKNNLERAEDIIYELKERIEPLREQSTRALKFIEI
                     QDNLKKYELNYLVHEYEKRSSQLNALKNQRDRSLEEKLKLQKRRDYYTEIIESQKEKI
                     NELQNKVNESEELRESKNKILENYNSLSQVHREKKLLYAQNIENVEGEIGLLEKRNRE
                     LKKNIDKLTQEKASSVEDLKRETEKLDGLNSDIKDYKKEIDEIILLIDLQKNELFEFH
                     KDINKNNSGKNTISSLIINNNDRIKHLNQEVNSGKEERKNRSEEVNRLQEEQILTRDI
                     LAQKKAELKNIWEELNCAEKDKEDTNKQIAGLSEDFSSSRSKMNILSNMEAYYEGYYK
                     SIKTVMNNKDKEPVLKAILGTVADIIWTEKKYEKAVEIALGSAIQNIIVNTESEAEEI
                     IEYLKNNKIGRVTFLPINMLAQRSLNSSEREVLKEKCVIDTADNLVHTNPLYEAVIKN
                     LLGRIIIVDNLNNGFIISRKLGNTVKIVTLQGDVINAGGSVTGGHIGANQALLGRKRE
                     IDELKTHIDRLSKELKEKNNSLDLLNIKIKGYKDSAEEIKSMINEKNNSYEMNNSKIK
                     MLSEQNEKTSSALDKYIEEIDFIQKEKQKYENDLKALNDETEKLKSRIAEKEKSIETA
                     IVQNDSKKLRYDEYNTVILSQRDKVAQINQNIKLLEEKLNSINTEIERNNEQKKYKEE
                     GLAAIYSDIDAARDTIAEYTEKAETLREEINALNESYAKEKDRLKTYRDEIYDYQNKI
                     NESNKEITDILDDEMAMNVKIERESSKIEDISAKLWEDYEINYAMALKYKDDSISQTK
                     LYSEVNSCKKAIKELGNINLDSIEEYKEVKERYDFLTKQQKDLTDAIDQLSEVIKELE
                     IQMKDKFVEEFSNIREKFSQVFKKLFNGGSGDIYLENEEDALNSNIEITVQPPGKKLS
                     KISLLSGGEKALTAIALLFSILKTKPTPFCVLDEIETSLDELNIFRFSQYLKEFSKET
                     QFIVITHRKGTMEYADTLYGATMEEKGVTKLVSLRLSDAV"
     gene            complement(9938..11020)
                     /locus_tag="PKK75_02095"
     CDS             complement(9938..11020)
                     /locus_tag="PKK75_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82418.1"
                     /translation="MNKKMKIISIFVPHEGCPNNCVFCNQRRITGQKNIVVDGEYVKN
                     TVEEYIRTIDKNIHIEIAFFGGSFTAIDIKVQEELLMAAKVFKDSGIINSIRCSTRPD
                     AINDEILRLQKNYGMDIIELGIQSLDDGVLKTSNRGHSKKQSMEASRLIKEHGFILGH
                     QIMPGLPGSSREKDIKTCLESIDMEPHIVRIYPTLVIKDTELFEMYEKGSYKPLTLEE
                     AAEVSAYMYSLYSVNNINVIRIGLQNTESIREDADVQAGPFHPAFRQLVEERIYSRVL
                     ESKLRRFDLKGKDIVIHAPLNLISNIAGHNKKNIKRLKENFLIKQISFKEKNDDIIEI
                     YHNDHILCSFNKSEIFENYLKMQQVD"
     gene            complement(11013..11720)
                     /gene="rnc"
                     /locus_tag="PKK75_02100"
     CDS             complement(11013..11720)
                     /gene="rnc"
                     /locus_tag="PKK75_02100"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNZ82419.1"
                     /translation="MNTYTVQDFEKVINYKFNNIKILEKSLTHSSYSNEDKSYNRINN
                     ERLEFLGDAVLSISVSRYIFDKFSDYPEGDLTKLRAQVVCEDTLSLAAGNINLGKYLL
                     LGKGEEASGGRERKSIQADAFEAVIAAIYLDGGYREAERFVLDNLTKYIKLAVKGKIV
                     TDFKSYLQEYYQSKSQFYKIRYVVTKEEGPDHEKVFHVNAVVNKNVAGKGTGKNKKEA
                     EQNAARDALIQEGQLNE"
     gene            complement(11827..12054)
                     /gene="acpP"
                     /locus_tag="PKK75_02105"
     CDS             complement(11827..12054)
                     /gene="acpP"
                     /locus_tag="PKK75_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNZ82420.1"
                     /translation="MFERIRELVAEKVGVDPEDITMETSFADDLEADSITLFELVMAI
                     EDEFDIEVDDESIEKIETVGDIVKYLEEINS"
     gene            complement(12060..13058)
                     /gene="plsX"
                     /locus_tag="PKK75_02110"
     CDS             complement(12060..13058)
                     /gene="plsX"
                     /locus_tag="PKK75_02110"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNZ82421.1"
                     /translation="MKIAIDAMGGDNAPEAAVIGSILARDEYGLGIILFGKEIEIKKI
                     LEKNTSNFNNIELINAEEIISNDDKPVLAIRRKKDSSMVKALYAVKNNEADALVSAGS
                     TGALLSGATLIIGRINGIERPAIGSLIPNMSGGVCLLIDSGANVDSKPEFLYDFAIMG
                     DIYLKRTMNIKSPRIGLANIGTEKSKGDKLTVETYQLLENSNLSLNFTGNIEARDILK
                     GNVDIVVCDGFTGNIILKTMEGTMLELMKGLKEEIMSSLRSKIGGALIKPALKSFKSK
                     FDYSEHGGAPLLGVKAPVIKAHGSSDSKAVKNAIRQAKICAEGNMNELIEESIMRR"
     gene            complement(13176..13355)
                     /gene="rpmF"
                     /locus_tag="PKK75_02115"
     CDS             complement(13176..13355)
                     /gene="rpmF"
                     /locus_tag="PKK75_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNZ82422.1"
                     /translation="MAVPKRRTSKAKRDSRRAANAKLTVPNTVECPQCHEPKLPHRVC
                     GGCGYYDGKEVVAVE"
     gene            complement(13368..13862)
                     /locus_tag="PKK75_02120"
     CDS             complement(13368..13862)
                     /locus_tag="PKK75_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNZ82423.1"
                     /translation="MLLKINKFLSSEAVSLTINENFSIDDEEFLKETGLDKDILLTGE
                     ILKIDDGTVFTGKIEYTFTDECARCLKEFDRRIETGFQAEIVKKEDMESDEIQLVAAD
                     GTIKMDETIKQLIYLSLPMKSLCSKDCKGICPSCGVNLNIEKCRCENSITDPRLEKLK
                     DLLK"
     gene            complement(13917..14909)
                     /locus_tag="PKK75_02125"
     CDS             complement(13917..14909)
                     /locus_tag="PKK75_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82424.1"
                     /translation="MDKLLDVQDLKVHFNIKRGFIEDIFSKDQVVVKAVDGVSFSIKK
                     GEILSLAGESGSGKTTVGKAVLNLVKPTSGKVLFNNEDISAKSEKELKIFRQNAQMIF
                     QDPYQSLNPKQSVIDIVSEPLSIHNITKSEKDKKERVEVALELAGLKPAGEYLYRYPH
                     ELSGGERQRVAIAGSIILNPGFIVADEPVSMLDVSIRIGIINLLMELRNKKDISFLFI
                     THDLSLAWLISDRIAIMYLGKIVEMGGPELIEGKCKHPYTKALIDVMPLAKAVKNRTK
                     NALKGETPNPVKIPSGCRFHPRCPHCMDICRVEEPLLANISEGHLAACHLNKLP"
     gene            complement(14902..15870)
                     /locus_tag="PKK75_02130"
     CDS             complement(14902..15870)
                     /locus_tag="PKK75_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012580202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82425.1"
                     /translation="MMELLKIDNLKTYFKTSKGIVRAVDGVSFSIERGQAVGLVGESG
                     CGKTTTALSIVNLLPENGKITEGSILLDGKDLTKLSKDRVRKQRWKNVSIVFQSAMNA
                     LNPVMKVGDQVIEAIMYHNHLTYEEARKKTKELFDLVEIDKNRIDNYPHEFSGGMKQR
                     VMIAMALACDPKLIIGDEPTTALDVIVQAQILNLLNKIKEELNTGLLIITHDLSILGE
                     VCDEIVIMYAGKIVEKGRTEQIYEEHLHPYTYKLFKSFPDIQGERILEEALMGRAPNL
                     SDHLPGCRFNPRCSFASDKCRIEEPKLKEASPGHYCACHYGGVWNG"
     gene            complement(15872..16750)
                     /locus_tag="PKK75_02135"
     CDS             complement(15872..16750)
                     /locus_tag="PKK75_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82426.1"
                     /translation="MKNSEHEIVYKGYKEFFNIFIHNKMAVAGAVILGVFITVALMGS
                     LVYPFDLQEFGTAGDVMNPPDRSHLLGTDDMGRDVLGNLIAGTRVSLLVGLLSTLISM
                     ILGTVLGIYAGYYDNIISTIIMQVTNFFLVIPWLPLMLVLTAILGTSIWNIIFVIGMT
                     SWPGTARIVRAQTLSIRERPFIERITAIGADTGHIMVKHILPNVFPLVFANTILVSAE
                     AILSETTLSFLGLGDVSRSSWGMMLHYAFESGAASLGAYWYFLPPGICVVLLVLSFTF
                     LGYALDEILNPKLKRR"
     gene            complement(16755..17732)
                     /locus_tag="PKK75_02140"
     CDS             complement(16755..17732)
                     /locus_tag="PKK75_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82427.1"
                     /translation="MNKQYVLKKIIYSLITIFAVLVFNYFLFRIMPGNPLQILMRNPN
                     ASAEAIEKTREMFGLDKPSYIQFFIYIKSLFQGDLGTSFLYKGQPVAQVIGNRILPTL
                     LMIGAAEILAIIVGIFIGVISAWKRGTKIDIVSLSFSLISYAMPTFWIGIVFVTIFSV
                     NLRIFPTSGMMTPGLSYADVIEKSSDVIKHMALPVLTMSLLLIGQYALVMRSTLVDVL
                     TEDYITTAYAKGFKDDYVIKKHAVPNAMLPMITIIAINLGLVIAGAIQIETIFSWPGI
                     GRLMYDAIKSRDYPLLQGIFLLITISVIVANLLADLTYGYLDPRVKSRS"
     gene            complement(17791..19554)
                     /locus_tag="PKK75_02145"
     CDS             complement(17791..19554)
                     /locus_tag="PKK75_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ82428.1"
                     /translation="MKKKIIIFVLISAMLISGTANITLAADETNSLRIGVTQEIDSLS
                     PFLSWSITGDETFMLIYDPLVRVDENMAPVPSLAESWSKSSDNLTWTFVLDENAKWHD
                     GKNVTAEDVKYTYEYIISSGLGMYAGGLEGISSIEAADEKTVVIKTNEPKANMLLSNA
                     PILPKHILETLTMEDLETWSNDSPVGSGPFKFEELNLGGFLKLVKNEDYFKGSPGLDE
                     IIFVLYANDDVMTQALKIGEIDAVVNFSASQLEELKGVPEIEAISAVAQGFTELSFNC
                     SEDSSSKGNKLLLDKNIRQAVEWAIDKQNILDVVYYGQGKTGTSLVPLGDYYHYEPKD
                     ELRSFNIEKANELLEKAGYKDTDNDGIREDEKGNKLSFVFTLRSETPEEIKAGQMIAA
                     NVEKAGIELKIETVDDGVLIDKIYSQDFDMFIWGWGTALDPTVILNVMSTREIGNLSD
                     CNYSNEEYDRLLLEQQTIMDDSQRRETVYQMQKILYDDAPYIILFYDNNLQAVRTDKF
                     EGWKRVPEDGSYFYNLTYTNYVNVKPKAQTEGTVPEGTQNQGESGSSNTALIVGAVVL
                     LVAGAAVLMMRKKKNTEEDDD"
     gene            complement(19610..20560)
                     /locus_tag="PKK75_02150"
     CDS             complement(19610..20560)
                     /locus_tag="PKK75_02150"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNZ82429.1"
                     /translation="MKIKPKRLEIGNKIGIVSPASPSYYKSDIDRGIKTLKEWGFKVE
                     PSKNLNKKTGFFSGRDIERAEDLNEMFERKDIDAIFVTQGGYGSAQVLKYLDFDLIRK
                     NPKIFLGFSDITSLLLAINKITGIVTFHGPGMTRFNDEELTDYTKEHLFKALDGQAPI
                     GQINLADRKKWINVINGGQAEGELIGGNLTLICSSLGTPYEIDTKDKILFFEEVDTEP
                     WIFDHMMCHLSNAGVLQKASGFVIGECARCVPRRLDPGFHSDISLEDILEFYLKPLGK
                     PVLHGLPIGHTKDLATLPIGVKAYINADDKIFSIEEAAVL"
     gene            complement(20774..21970)
                     /locus_tag="PKK75_02155"
     CDS             complement(20774..21970)
                     /locus_tag="PKK75_02155"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNZ82430.1"
                     /translation="MNVLVINCGSSSLKYQLINMDGEKVLAKGLVERIGISGGLLKHE
                     KPGFDKLVIEEDLKNHKEAVNLMLKALLDEKYGAIRSMDEIDAVGHRVVHGAEKFANS
                     ALIDDEVIDTMKECISLAPLHNPPNITGIEACKELMPDVPMVAVFDTAFHQTMPPVSF
                     IYPLPYELYEELKIRRYGFHGTSHQYVSERVADLTGKSLEGTKIVTCHLGNGASLTAI
                     KDGKSFDTSMGMTPLEGLVMGTRCGDIDPAIVTFLINKKNMTGEEVDNLMNKKSGVLG
                     ISGVSNDFRDIEKEAGKGNTRAQLALDKYAYTVRKYIGSYAAAMGGLDYLVFTAGLGE
                     NSPPARKQVCEGLEFLGIEIDDEKNNTRGKEAEISKDGSKVKVFVIPTNEEVMIARDT
                     KKLTSK"
     gene            complement(22013..22588)
                     /locus_tag="PKK75_02160"
     CDS             complement(22013..22588)
                     /locus_tag="PKK75_02160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ82431.1"
                     /translation="MIYLVRHGESEANISRRFSGITDVELTEKGLLQAVKAGKKLKDK
                     TIHKIFSSPLKRAIYTAEIIADEIGFDRKDIIIENSITEVDFGIFENLTWEEIVEMYA
                     DEVESWTEFKHKYKFPKGEGYDDIVKRVAGFMDKVPDNSLISTHYGVIQAVLLYYNIA
                     DDDTLWNYKISNCDIFELDNKKLTGIIRSCT"
     gene            complement(22585..23322)
                     /gene="cobS"
                     /locus_tag="PKK75_02165"
     CDS             complement(22585..23322)
                     /gene="cobS"
                     /locus_tag="PKK75_02165"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNZ82432.1"
                     /translation="MASGFLTLISFFTRIQVGRKIEYNEENFIRALNLFTLTGAVIGF
                     LLALTYLIFNRQIPFLRGLAITIMYILITGGIHLDGAADTSDGLFSGRTGDEVFEIMS
                     DSRIGSFGVITLILIVLSQFILFSYISIEAVFIMPLAGRAGAIAGCWNKKYAKGTKGM
                     GTAFVENIDKSVLLINLSILFVFSILSWQRTIIFTASYAAVSLSYFISTLIEDKIGGM
                     TGDTCGFITEISQIIFMITVLFLMRQL"
     gene            complement(23304..23897)
                     /gene="cobU"
                     /locus_tag="PKK75_02170"
     CDS             complement(23304..23897)
                     /gene="cobU"
                     /locus_tag="PKK75_02170"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNZ82433.1"
                     /translation="MSLTFVIGGARSGKSTFAEAKAKEYSQHVLYIASAVVTDQAMAD
                     RVKRHREQRPASWHTLEAYGSFDKLIDNPLFVQSQVILFDCITTMIANYMVDSSLDFD
                     TCSVEDVNILEEKITSEVFSLIDVCNNNNKKLIIVSNETGMGLVPSYYMGNYFRDISG
                     RINSRLGLKSDNMYFIFSGIPIKLKDKGEMVKWPQDF"
     gene            complement(24064..24243)
                     /locus_tag="PKK75_02175"
     CDS             complement(24064..24243)
                     /locus_tag="PKK75_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82434.1"
                     /translation="MQNSNSSQNYNFSNNVKQFELNEAKKKMGISQVQGQIPPVQQKS
                     NQLNKTDEKEFNTLF"
     gene            24482..25681
                     /locus_tag="PKK75_02180"
     CDS             24482..25681
                     /locus_tag="PKK75_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNZ82435.1"
                     /translation="MKITGVVAEYNPFHAGHEYQLKMARKLSDCDCIAVVMSGNFVQR
                     GEPAIIDKFNRAEAAVYGGADIVIELPMPFSCQNAEMFAFAAIKELKKLNVDSISFGC
                     ENDDVKLLEKIACLQLTPEFNKNFKEEIKKGLSYPNAMSNALVSLINDEAEAVYSPNN
                     VLAVEYIKSAMKLDLNLSYYPVKRLGMAHNDTEFQGPFHSAAAIRNSMLEGSLNNLKL
                     TEKSLELIEKFYNDHKQFNTLNNYTDYLYYKIITCDDLNTIYEIKEGLNNKIMSQSFK
                     HENIDDLIMSLKSKRYTYSKLRRCLLNILLDIKKVDIIKIMLTNNDYVKVLAFNNAGR
                     RAIKHAKNNGIKVINRFSDYKKYNLSPENLKVFNITNKSSNIYYLPLKNRKINSEYTE
                     NAVYINI"
     gene            26023..26973
                     /locus_tag="PKK75_02185"
     CDS             26023..26973
                     /locus_tag="PKK75_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82436.1"
                     /translation="MLKRNFIPVSIMMIIFLFVMKPDLVSEGVFNGLKIWVNNMIPYL
                     LPMAILSNILLQYNFLYRLSEKLSFISNIIFQSRYAFIPYFISFVVGYPSGAMTVNTM
                     ASYKRIDSSEANALVVFTNNCSFQFMAGAVSYSMLGDLSLSKYIALPHLLSALLIGML
                     IKKDRPTAAFAKDKKQKYISFYDAFNSSIYKSITSILSIGGVIVMFSVFSNFFDNLLI
                     SLSKYLLLSRPLSDIIHSVSVGILEISNGCSLAASSSLPLDVKLIVINFLISFSGISV
                     ILQTTAVTDDFNFNISSYVFYKALQGLLSVIICIGMLILL"
     gene            27080..27988
                     /locus_tag="PKK75_02190"
     CDS             27080..27988
                     /locus_tag="PKK75_02190"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNZ82437.1"
                     /translation="MINVTLIGTGGMVPLPNRYLASCHIECNGKSILIDCGEGTQISL
                     HKGKISLNKIDTILITHCHADHVTGLPGLLLTMGNHGRTEPLDIIAPRGSTRILNSLL
                     VVCGFLPYEVRIAELHDTKPQEFIKIGLYITSIPLKHHINCLGYSFELRLKARFQPDK
                     AKDLNIPVNLWRQLHEGGEVVVEGRTITPSMVLGSPRKSIKVSYVTDTRPVKFIDEIV
                     RDSDLFICEGMYGNDEMLEGAVAKMHMIFSEAAYIALNAKVQELWLTHFSPSMPNPEE
                     YLKFASKIFPDTLIGSDLMSKTLKSL"
     gene            complement(28088..29563)
                     /locus_tag="PKK75_02195"
     CDS             complement(28088..29563)
                     /locus_tag="PKK75_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNZ82438.1"
                     /translation="MEQEKRGQWGTNFGFLMAAIGSAIGLGNIWGFPYKMGANGGFIF
                     LIIYLLLVSFVGFATMLGELTLGRKYGMGVIGTYRKISEKYTWIGWLGWLSGFLILAF
                     YSVLGGFTIRYCIGFLMEMFSAGAGFGGLDSGGFFGSLILSAPMSILFHAIFMGATIL
                     IVMGGVQGGIEKFTAVAMPTLFVMVIIVVIRSVTLEGAFVGLEFMFKPNLDIIKEVGL
                     MRVLKIAAGQMFFSLSLGMGCMITYGSYLSKKENLEWNAMLIPLADTSFAVLSALAVL
                     PAVGAFMYQGVEGVSFGAGPGLLFVTLHRVFSVGMGGFMGNFFGAFFYFLVFIAAVTS
                     SISLLEVCTAFFIDKAVEKGEEPNRKKYTLILGLVIFFLGFPVAMDGLGAGKAGGAMI
                     DIPATIFGMKEVRVAFDCWLDFYDMISEGVFMPLGALLMSLIIGWKLKTTVIKEEVEA
                     SPGIKMKRYGFWDICFKFIVPVAMVLVLMGQLDDFFGLGIF"
     gene            complement(30005..31354)
                     /locus_tag="PKK75_02200"
     CDS             complement(30005..31354)
                     /locus_tag="PKK75_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNZ82439.1"
                     /translation="MSTKNEVFGYLPDERPPLLGLIFFALQQIVVMFPATVLVALITG
                     FHVSTTIFASGLATLGFILITGRQIPLYYGSSFSYIAAIASIMSAEAFASYSLNDKIS
                     VAQFGIVMSGFVSILAGYIINKSGKDKIEKVLPATVTGSIAIIIGLSLAANAMTNAAA
                     FPEAALGNPNAGSWAWTIAIITLFSTILYSVYLKGKLSQLPILFGLLTGYAAALTIGG
                     VTGIPFVSFDTIQTTGILNLPIFTFPKPTLAAVVAIMPIAIATIPESTAHVYQLDIYV
                     NDLAKKKGINKKYDIENKLGLNLIGDGMGDIISGFIGGPAGTNYGENISAMAISKNFS
                     IYVLMLAAVFTMIISCFTPLINIVYSIPTAVIGGLSIYLFGVIGAQGITIMIDRKVDM
                     FSSKNLAVIAVILIVGLGGSFGFEGGMIPMFGIELPAIATAAVTGIILNLMLSINEK"
     gene            complement(31556..32215)
                     /locus_tag="PKK75_02205"
     CDS             complement(31556..32215)
                     /locus_tag="PKK75_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ82440.1"
                     /translation="MKKDLTELVFILDRSGSMSGLEKDTIGGFNSMLAKQKSEPGDAL
                     VTTVLFDHDYEMLHDREKIKNVDEISEQEYFVRGSTALLDAMGRTINYIGKSISDTGE
                     DDHPGKVIFVIITDGMENSSHEFSKAKVKRMVEHQKTKYSWEFVFLGANIDAVKTAAD
                     FGIDEDRASNFCADGQGINLNYSAVSTFVSEMRMGKKVDSSWKSKIDEDYKKRGKKGP
                     S"
     gene            complement(32291..33286)
                     /locus_tag="PKK75_02210"
     CDS             complement(32291..33286)
                     /locus_tag="PKK75_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNZ82441.1"
                     /translation="MPFEIIRNDITRVKADAIVNAANSALKMGGGVCGAIFRAAGAED
                     LQAECDKIGGCNTGEAVITKGYKLPAEYIIHTVGPIYNGGNSNEEELLKNAYLNSMKL
                     AKDHKLESIAFPLISSGIYGYPKDEALKVAISAISEFLLENEMTVYLVMFDTKAFAFS
                     EKLFSKIRKYIDDKYVEDHKLLDRGRVLQEYEFNYAAAPSLKSVKEQKARSLEDVVKQ
                     LDETFSEMLLRLIDEKGMTDVETYKKANIDRKLFSKIRTDKNYKPRKNTALAFAVALE
                     LNVDETRDLLEKAGYALSRSSKFDLIVEFFLEEKIYNIFEINEALFAFEQPLLGL"
     gene            33478..>33867
                     /locus_tag="PKK75_02215"
     CDS             33478..>33867
                     /locus_tag="PKK75_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HNZ82442.1"
                     /translation="MAQLFVVLIVFLLIPLLGRFKLKLSYILLISCGVLALFSGMGAQ
                     AAWQSVTNIFTDFSSLNTILSVMMVSILGGLMKNYKILDRIVSSIYQIIQNKKIILMI
                     LPALIGILIVPGGALLSAPFIYEMGEEL"
BASE COUNT         9261 a         7211 c         5390 g        12005 t
ORIGIN      
        1 cgctctcagg tagtctattt gagttttatt tattaaaaag ccctttttat aagctatatt
       61 gtctaaaata tctaaaggct tctcccctgc ctctactccg tatctttcaa gaatgctatt
      121 ttctttcccc atctgctgca tcttttcaac aaatttaagc gcaatttctt ccaataccat
      181 aacttcatcc cttattgcac ctgtgaaggc aaggtttaat gcatattctt catcatcaaa
      241 ttttggccac agtatgccgg gagtatccag caggtcaata tttcctgcca ggtgtatcca
      301 ctgcttgccc tttgttacac cgggcatgtt tcctgttctg gtgcttttct ttttagccag
      361 tgaatttata agtgttgatt ttcccacatt gggtatgccc acaatcattg ctttgattgt
      421 tttttttacg aatccctttt ttttgtatct ctcaataatt tccctggttg cttctgcaat
      481 tgcactgtta agcctgtcaa gctccttatt gttcgtggaa ttataaagta caactgacca
      541 gcccctgctt ttatagtaat cttcccattc tttattcatg acggggtctg acaagtcgta
      601 tttgtttaat actatcagcc tttttttatc cctgaacaat ttgtcaaagt cagggttttt
      661 actgctataa ggaattcttg catctacaac ttcgattatt aaatttacta atttaatatt
      721 ctcctgaagc aattccttgg tctttttcat atggccggga taccagttta tgttcatatc
      781 tacctctcac aacggggaca ttcctctatt ggctgccttg catagagtat ctcacaatag
      841 aggtgtgtcc ccataccttt aaaaagggac atgcctttaa gttctgaaag tctatggaat
      901 agactcttaa aggaatgtcc ccatcaggcg aggacatccg tcctcaatca aattaatact
      961 ttcttaattc tttaactttt gttgctttac ctgttctttc tctcaagtag aacaaacgag
     1021 cacgtcttac tttacctttt cttacaactt cgattttttc aattttcggt gaatgaacag
     1081 ggaatgttct ttcaactcct actccgtagg aaatttttct taccgtgaaa gttgctctgc
     1141 tgctttcgcc ttgaatttta attacgatac cctcgaatac ctgaattctt tctctgtttc
     1201 cttctttgat tctaacatgt acctttatag tatctccaac attaaaagct gtcacgcttc
     1261 taatttgttc gttctctatt gtttttatta agtccatttg tatacctccc ttcgccttag
     1321 cgttcttgtt acctgttcat aggaccgccg tatactctta agcattatag cacatcaata
     1381 ataatgcata caacatttat ttatatttct ttgataatgc cttgtacttt tctcttatac
     1441 tctcgtcctt tatcatatcg ggacgtctct tcacagttat ttctatagcc ttgaaaagcc
     1501 gccattcttc aatatttttg tgatgtcctg acaataatac ttccggcacg tctaatccca
     1561 tgtagctttc cggtcttgtg tactggggat actccagcaa attttcgctg tgtgattctt
     1621 ccctggaaga ttcctccgag cccagcaccc ctttaaggag cctgcctact gcatcaacaa
     1681 tcattaatgc cggcagctca ccgcttgtta aaacataatc tcccacagat atttcctcat
     1741 ctacatattt gtcgataatt ctttgatcta tgccttcata atgtcccgga ataagtatta
     1801 tgtgctccat gacagacagt tctttagctt tattttgatt gaataaagag cctttcggtg
     1861 ataaataaac aatataggaa ttatgccttt taatagcatc caaagcttta aacatgggtt
     1921 ccggtctcaa aagcatgccg ggacctccgc cataaggata gtcatctact tttttatgtt
     1981 tgtcttcgga ataatcccta agataaacat attccaattc aaacaatttg ttttctacag
     2041 ctctgccgat aattccgtta tttttaaaaa cttcaattaa ctccggaaat aatgttatta
     2101 tttcaaattt cattccaaca acccttcaat aacatcaatt atcatttttt tatccttaac
     2161 actgactgat ttcacaacat cctttactgc aggtatcaaa aatgatcttt ccttgttttt
     2221 tacttcatat acatcgttgg ctccggtttg ataaatttct gaaattttac ccagatattc
     2281 accggtgctt gaatacacat ccatcccatc caaatcaaat aaataatatg aatcatcggg
     2341 aagctttctc aactggtcat ccagtatgga aatgtatgtg tccttgaatg tttctgctgt
     2401 ttccatatca tctatacccc aaagctttaa ataaaccatc cctttttgat accatacatg
     2461 gtggattatt ctttttatgc tgtctttttc agtatagaca tattcaagct cttcaaatct
     2521 ttccggctca tcagtcatag gcatgcattt tacatagcct cttacaccgt gagtatttat
     2581 aattttgcca actttaatat aatccttcat ttttgcctca aagcttattg aattatttca
     2641 acaataactc ttttattatc ctttgtggca gctgctttaa caacagttct tatagctttt
     2701 gctattctgc cttgctttcc aattacttta cccatgtctt cgggagctac ttttaattcg
     2761 attatcagtg attgactacc ttcaacttca ttgacttcaa ccatatcagg attatcaaca
     2821 agtgattttg ctatatattc tactaattca cccatattat cacctcttat ttgttttcat
     2881 ctcttttttc ataaatgccg ttattcttga aaagcatatt tactgtatct gttggttttg
     2941 caccgttgtt aagccattta attgcacttt catcatttat tttcacaacc ttaggctctg
     3001 atacaggatt gtagtaccca atttcttcaa taaaacggcc gtctcttgga gaacgagaat
     3061 ctgctactac tattctgtaa aaaggtttct ttttggaacc cattcttttt aatctgattt
     3121 ttactgacat actgtcacct ccttatttaa tgtgcgggga tacactcatt ccataaagcc
     3181 aggctttaaa gacaggtgaa tatctcctgt tttgttaata atatatatga aaaaggaaaa
     3241 ttcctatttc taacacagtt tcatttatag agcggcaacc cacagtctca ttcctagaat
     3301 ggcaacttga atccacccaa gcccccgaag ccttttttcc tgcctgttcc catcgaagag
     3361 aattttttca tcattttttt catctcttca tattgcatta acagggaatt taccttttga
     3421 acagaagttc cgctgcctgc tgcaattctt tttcttctgc ttccgtttat aatcgatgga
     3481 ttcagtcttt ccttcttggt catggattga attattgctt ccgtatatac cagttctttt
     3541 tcatcaacct taagcccctt cagcttttta ttgcctccaa gtccgggaac catttccagt
     3601 atttcatcca aaggtcccat attcttcatc tgctgaagct gggccaggaa atcttccaaa
     3661 ttgaagcttt gtgtctttaa ctttttttca agttcaactg cctgtttttc atcaaatgca
     3721 ttctgggcct tttcaataat tgaaaggaca tcacccattc caagtattct tgatgccatt
     3781 ctgtccggat gaaaagtttc aagctgatcc attttctccc ctacagataa gaactttata
     3841 ggtttatcca caacgtttct tatagaaagg gctgcaccgc ctcttgcatc accatcaacc
     3901 tttgtgagta taactccggt tatatccagg tatgaattga atgtctctgc aacctttacc
     3961 gcatcctgcc ctgtcattgc atccaataac aagagtattt catcaggctt cactgtttct
     4021 ttgattttta ccagttcatc catcaaatca tcatcaatgt gaagtcttcc tgcagtatca
     4081 ataattacca cgtcattacc gtgcttcatg gcgtggttca tagcttccct ggcaattgtc
     4141 acaggagata ttttatcccc catttcaaaa acagggacat caacgctttc tccaactacc
     4201 ttaagctgct ttatagcagc cggtctgtat acgtcgcagg ctgcaagcaa aggtctcctg
     4261 ccgtcttttt taagttttct tgcaagcttt cctgttgctg ttgtttttcc tgcaccctga
     4321 aggccgcaca tcataaaata tgttatattg tttgaattaa attttaactt gctttccccc
     4381 tggcccatta ttctggccag ttcttcatgc actattttta caacctgctg tcccggggtt
     4441 aagctttcca ttacaccggc acctaaggat ctttctttca cattgttaat aaaggatttt
     4501 acaaccttaa aattaacatc tgcctcaaga agagacattt taatttcctt cattgccagg
     4561 tcaatatctt tttcagttaa ttttcccttg cctctaagct tttgtaatgc actttgaagt
     4621 ttatcggata aattttcaaa catctttaac cctcattaat tatttttgca gcttcagtct
     4681 ttattttttc aataatatct ttatatttgt cctcatcaat cattgatgaa agttttatga
     4741 tgttttcagc tgcgtcgctg aatttaacat atttatcatg gagtttgatt ttcttttcgt
     4801 agtctgaaag attcttctcc gctcttttta aagcatcgta gactccctgc ctgctgatgt
     4861 ttaattcatc ggcaatttct gacaggctgt aatccaagtt ataatacatt tcaattatgg
     4921 ctgcctggtt ttccttaagc agttggccat aataatcata caggatgcta tatataaagc
     4981 atttctctat catatccgcc tctgtaaagt attttgcttg acagtgaatt ttacaatata
     5041 taatacgctt tgtcaatagt ttttactaga aagtttattg tcttttttat ggatgggact
     5101 tatagttcac gatgacaaaa aaaaaataat aaataataag cgaacatttg caggacacga
     5161 tttataattc taagcgaatg aaggctaaaa atccgtttgg cagcttgcac tgtttgagca
     5221 gagcgagtat gcaagctgca ggatttttag ctgtaatgag ctttagaatt attcgtgccc
     5281 gaaaccgtga gcgtttattt ttattatttt ttgctgctag gctattttca aaaaaggact
     5341 ctgagagatg tggttaggac atcttgcagt ctcattcaaa aattgcattt atgaaatctt
     5401 gagcgtcgaa atcttgcaga tcgtctattt gttctcccac accaacatat ttaattggta
     5461 ttttaagctc gtttactata gggaatgcta tgcctccctt tgctgtgcca tccaatttag
     5521 ttaaaatcaa gccgttaata ttgcatgcct ctttaaagac ctttgcttgt gctatcccat
     5581 tttgaccagt tgtggcatcg ataaccagat acacatcaat gtgggcttcg ctgaattctt
     5641 tatcaagaat tttgaaaatt ttactgagct cattcatgag atttttcttg ttgtggagtc
     5701 tgcctgctgt atcacatatg agtatatctg cttttcttgc ctttgttgcc tgtatggcgt
     5761 caaaaactac tgagccgggg tctgagccat agtcagatgc aattatatca acgcctgccc
     5821 tctggcacca ctccttgagc tggtctacag ccgcagccct gaatgtatcg gcagctgcta
     5881 caataacctt attgccggct tgctttaaac ggtatgcaat tttcccgatg gaggttgttt
     5941 tgcctacgcc atttacaccg actatcagta ttaccctttg tttgtcggtt gtatcaggtc
     6001 tttcattttc cttgagtgta tttataaggt actctttaat tacatcttta atttcaaggg
     6061 tgtcttccaa ttttctcttt tttgtttctt cctttatgta ttcaataatg tccattgttg
     6121 tttctacacc catgtctgat gtaatcagta tttcctccag ttcgtcaaga aaatcatcat
     6181 ctattctttt atgttggaaa ataacacttt cgatttgttc ggtaagattt ttcttagtct
     6241 ttgtaagtcc ttcttttaat ttagcgaaaa agcctttttt ttcattcatt tcttcctccg
     6301 taaatatggt atattttttg ggctattcct ctgtcccgca gagacttgcc tcatagtaga
     6361 ggtgtgtccc cataccgcta taccgcatca gataacctta gcgatactaa ttttgtaact
     6421 cccttttcct ccattgttgc cccgtataaa gtgtcagcat actccatggt gccttttctg
     6481 tgggttatta ctataaattg cgtttccttc gaaaattcct ttaaatattg tgaaaatctg
     6541 aaaatattca attcgtccag tgatgtttca atttcatcca atacacaaaa gggtgtgggc
     6601 ttggttttta aaattgaaaa caaaagggca attgcagtaa gtgccttttc gccgccggac
     6661 aacaatgata ttttgcttaa ttttttcccc gggggctgta cagtgatttc tatattgctg
     6721 tttaaagcat cttcttcatt ttccaaataa atgtctccgc tgcccccgtt gaaaagcttc
     6781 ttgaatactt gagaaaattt ttcccttatg tttgaaaatt cctccacaaa tttatccttc
     6841 atttgaattt caagctcttt tatcacttcg ctgagctggt cgatggcatc tgtcaaatcc
     6901 ttctgctgtt ttgtcaagaa atcatacctt tcctttactt ccttgtattc ctctatggaa
     6961 tccagattaa tatttcccaa ttcctttatg gcttttttgc atgagtttac ttcgctgtat
     7021 agcttggttt gacttatgct gtcatccttg tatttcagcg ccattgcata gtttatttca
     7081 tagtcttccc acagctttgc agaaatatct tcaatttttg aactttctct ttcgatttta
     7141 acgttcatgg ccatttcatc atcaagtatg tcggttatct ctttatttga ttcattgatt
     7201 ttattctggt aatcgtaaat ttcatcccgg taggttttaa gtctgtcttt ctcctttgca
     7261 tagctttcat ttaaagcatt tatttcttct cttaaagttt cagctttttc agtatattca
     7321 gcaattgtat ccctggctgc atcaatgtcg ctgtaaatgg ctgctaagcc ctcttccttg
     7381 tactttttct gttcattgtt tctttcaatt tcagtattaa tactgttgag tttttcttct
     7441 aaaagcttga tattttgatt gatttgtgca accttgtccc tttgacttaa aataacagtg
     7501 ttgtattcat cgtatctaag ctttttggaa tcattctgaa ctattgcagt ttcaatgctt
     7561 ttttcttttt cggcaatcct tgacttcagc ttttccgttt catcattcaa agcttttaag
     7621 tcattttcat acttttgttt ttccttctgg atgaaatcaa tttcttcaat gtatttatca
     7681 agggcagaag atgttttttc attttgttct gacagcattt ttatttttga gttattcatt
     7741 tcataagaat tatttttttc atttatcatg ctttttattt cttctgctga atccttgtat
     7801 ccctttattt ttatgttcaa gagatccagc gaattatttt tctcttttaa ttcctttgaa
     7861 agcctgtcga tgtgagtttt caattcgtca atttctcttt tccttcctaa aagggcctgg
     7921 tttgcgccta tatgccctcc tgtaactgac cccccggcat ttattacatc tccttgcagt
     7981 gttacgattt taactgtatt gcctaatttc cttgatataa taaatccgtt gttaaggttg
     8041 tccacaatta ttattcttcc aagcagattt tttattacag cttcatatag ggggtttgta
     8101 tgaactaagt tgtctgctgt gtcaatgaca cacttttcct ttaaaacttc tctttccgag
     8161 ctgtttaaag atctttgcgc aagcatgtta ataggaagaa atgtaactct cccgattttg
     8221 ttattcttca aatattctat tatttcttcg gcttcacttt cagtatttac aataatattt
     8281 tgtatggcag atcctaatgc tatttcaacg gctttttcat acttcttttc tgtccatatt
     8341 atatctgcta cagtacccag tatggctttt aataccggct ccttgtcctt gttgttcatt
     8401 actgtcttta tgcttttata gtagccctcg taatatgctt ccatattgga cagtatgttc
     8461 atttttgatc tggatgagct gaagtcttcg ctcagacctg caatctgctt atttgtatct
     8521 tccttatcct tctctgcaca gtttaattct tcccatatgt tttttagttc agctttcttt
     8581 tgagccaaaa tgtctcttgt aagaatttgc tcttcctgaa gcctgttaac ctcttcactt
     8641 ctgttttttc tttcttcttt ccctgaattt acttcctggt ttaaatgctt aattctgtca
     8701 ttgttgttta ttattaatga gctgattgtg ttttttcctg aattgttctt gttgatatcc
     8761 ttgtgaaact caaagagctc atttttttgg aggtcaatca agaggataat ttcgtcaatt
     8821 tcctttttat aatcctttat atctgaattc aaaccatcaa gtttttctgt ttctcttttt
     8881 aggtcttcta ctgatgaagc tttttcctgt gttaatttat caatattttt ctttaattcc
     8941 cggttccttt tttccagtaa ccctatctca ccttcgacat tttctatgtt ttgtgcatac
     9001 aagagtttct tttccctgtg aacctgactt aagctgttat agttttccag aatcttattt
     9061 tttgattctc tgagctcttc actttcattc actttgtttt gaagctcgtt tattttctcc
     9121 ttttgggatt caataatttc tgtatagtag tcccttcttt tttgaagctt gagcttttcc
     9181 tccaatgacc tgtccctttg attctttaag gcattaagct ggctgcttct cttttcatac
     9241 tcatgaacca aataattaag ctcgtacttt tttaaattat cctgtatttc aatgaatttc
     9301 agggctcgtg tgctctgctc acggaggggt tcaatccgtt ctttcagttc atatataata
     9361 tcttcggctc tttcaagatt gttctttgtc ttttcaagtt ttttttctga ttcttctttt
     9421 cttgacttat attttacaat gcctgaagct tcttcaaaaa cctgtctcct cacatcggaa
     9481 ttagggctta gaatatcttc caccctgcct tgtccaataa aagaatagcc ttctcttcct
     9541 attcccgtat ccataaaaag ctctctgatg tctttaagcc tgcattgctg cttatttata
     9601 gagtattcgc tttcaccggt tttataaagt tttctgctga tgcttacttc cttgtattca
     9661 ataggtatcg tgccgtcttc attgtcaaaa gtcagcctta cttcagcata gcccaagggc
     9721 agcctctttt cggttccggc aaaaataata tcgtccattt tgcctcccct gagagtctta
     9781 gcgctttgct ctcccaagac ccatttaatg gcatcggaaa tgttgctttt cccgcttcca
     9841 tttggcccca ccacggctgt aatacctttt tcaacgtcta tttctgtttt ttccgcaaag
     9901 gatttaaatc cgtttacata tatttttttt aagtacatta atccacctgc tgcattttca
     9961 gataattttc aaatatttct gatttgttaa agctgcacaa tatatggtca ttatgatata
    10021 tctcaataat atcatcattt ttttctttaa aggatatttg ttttattaaa aagttttctt
    10081 ttaatctttt tatgtttttc ttgttatggc ctgctatatt gcttatgaga tttaaaggtg
    10141 catggattac aatgtcttta ccctttaaat caaaccttct gagttttgac tccagtacac
    10201 gggagtatat tctctcttcc accagctgtc tgaaggcagg gtgaaagggt cctgcttgta
    10261 catctgcatc ctcccttatg gattcagtat tttgcagccc aattcttatg acatttatat
    10321 tgttgacact gtaaagagaa tacatgtatg ctgacacttc cgctgcctct tccaatgtaa
    10381 gcggtttgta agaccctttt tcgtacatct caaaaagctc tgtgtccttt attaccaggg
    10441 ttggataaat tctcacaata tgaggttcca tatcaatgga ctcaaggcag gtctttatgt
    10501 ctttttctct gctgcttccc ggaagcccgg gcattatctg gtggccaaga atgaatccat
    10561 gttcttttat gagccggctt gcttccatgg actgcttttt actgtgtccc cggtttgaag
    10621 tcttcaaaac cccgtcatcg agactctgaa ttcccaattc aattatatcc ataccgtaat
    10681 ttttttgaag tcttaaaatt tcgtcattta ttgcatctgg tcttgtggaa catctaatgc
    10741 tgtttataat accagagtct ttgaagacct tggcggccat taaaagttct tcctggactt
    10801 ttatatcaat tgccgtaaat gaacccccga aaaatgcaat ttctatatgg atattcttgt
    10861 caattgttct tatgtattcc tcaactgtat ttttaacata ttccccgtcg acaacaatgt
    10921 ttttctgtcc tgtaatcctt ctttggttgc agaatacaca attgttggga cagccttcat
    10981 gagggacaaa aatagaaata atcttcattt ttttattcat tcagctgtcc ttcctgaata
    11041 agcgcatccc tggcagcatt ctgctcggct tcctttttat ttttgccggt gccctttcca
    11101 gccacatttt tgttaaccac cgcatttaca tgaaatacct tttcgtggtc aggcccttct
    11161 tcctttgtta ctacatacct tattttataa aattggcttt tgctctggta gtattcctga
    11221 aggtatgact tgaaatccgt tacgattttt ccttttactg caagctttat atactttgtt
    11281 aaattatcca gtacgaatct ttcggcttcc ctgtatcctc catccaggta aatggcagct
    11341 atgacagctt caaaagcatc tgcctgtatg gattttcttt ctctgccccc tgatgcttct
    11401 tctcccttcc ccaaaagcaa atactttcct aaatttatat ttccggctgc caggcttaat
    11461 gtgtcctcgc agacaacctg ggccctgagc tttgtcaggt ctccttcggg ataatcagaa
    11521 aacttgtcaa atatatatcg gctcaccgag atgcttaaaa cagcatcgcc aaggaattca
    11581 agtctttcgt tgttaatcct gttatatgac ttgtcttcat ttgaatatga gctgtgggta
    11641 agggattttt ccaatatttt tatgttgttg aatttatagt ttatcacttt ttcgaagtct
    11701 tgcactgtat atgtattcat ctttcctccg gatataattg cttattacta taatgaatta
    11761 tttcattaaa ttaataaata attataaatc gggggacatt ccattttggt gtgcccccat
    11821 acctatttaa ctatttattt cttccaggta ttttactata tctcctaccg tttcaatttt
    11881 ttcaatgctt tcatcgtcaa cttcaatgtc aaattcatcc tcaattgcca tgaccaattc
    11941 aaaaagtgtt attgaatctg cttccaaatc atctgcaaag gatgtttcca ttgttatgtc
    12001 ttccgggtca acgccaacct tttcagctac caattctcta attctttcaa acacattctt
    12061 cacctcctca ttatgctttc ttcaattaac tcattcatat tgccttcagc acagattttt
    12121 gcctgtcgaa tagcattctt aactgccttt gagtcagaac tgccgtgtgc ctttattacc
    12181 ggagccttga ctcccaaaag cggagccccg ccatgttctg aataatcaaa tttgctttta
    12241 aagctcttaa gtgccggttt tatcaatgca ccgccgattt ttgaccttaa gcttgacatg
    12301 atttcctctt taaggccttt catcagctcc agcattgttc cttccattgt ttttaatatt
    12361 atgtttcctg taaaaccgtc gcatactact atgtcaacat ttcctttaag tatgtctctg
    12421 gcttcaatat tgcctgtgaa attaaggctt aaatttgaat tttcaagtag ctggtatgtt
    12481 tccacagtaa gcttgtcgcc cttggatttt tccgttccta tatttgccag gcctatcctt
    12541 ggcgatttga tattcattgt tctttttaaa taaatatcac ccataattgc aaaatcatat
    12601 aaaaactcag gctttgaatc cacgtttgca cctgaatcta tcaataaaca gactcctcct
    12661 gacatgttag gtatcaatga ccctattgca ggcctttcaa ttccgttaat tctgccgatt
    12721 ataagggttg ctccgctcag taaggctccg gtgctgcctg cggatacaag ggcgtcagct
    12781 tcattattct ttaccgcata taaagcttta accattgatg agtctttctt ccgtcttatt
    12841 gccaaaacag gtttgtcatc gttcgatatt atttcttctg cgttgatgag ctcaatatta
    12901 ttgaagtttg atgtgttttt ttccagtatt tttttaattt cgatttcttt tccgaaaagt
    12961 ataatgccca ggccgtattc atctcttgcc agtatactgc ctattacggc tgcctcaggt
    13021 gcattgtcgc cgcccatggc atcaattgca attttcatat ccacctctaa ctgatatact
    13081 tacaatagta tatagcagta atatttgttt tgcaataaaa aaatagtgcc aggcactatt
    13141 ttttacagcc ctaaccaatt attttgagac tttttctatt ctacagctac cacttcttta
    13201 ccgtcataat atccgcatcc accgcatact ctgtgcggta gcttcggttc atgacactgc
    13261 gggcattcta ctgtatttgg aacagttaat ttcgcatttg ccgctcttct tgaatctctt
    13321 ttggctttgg aagttcttct ctttggtact gccattttta cacctcctta tttcaacaaa
    13381 tcctttagct tttcaagccg cggatctgtt atactgtttt cacacctgca tttttcaata
    13441 tttaagttta caccgcagct tggacatata cccttgcagt ctttactgca taaagatttc
    13501 atgggcaggg acaaataaat taattgtttt attgtttcat ccatttttat agttccatct
    13561 gctgcaacca gttgaatttc atcggattcc atatcttctt tcttaacaat ttctgcctga
    13621 aatcccgttt caattcttct gtcaaactcc tttaaacacc ttgcgcattc atcggtgaaa
    13681 gtatattcta ttttgcccgt aaacactgta ccgtcgtcaa tttttaatat ttcaccggtt
    13741 aataatatat ctttgtcaag acctgtttct tttaaaaatt cctcatcatc tattgaaaaa
    13801 ttttcattta ttgtcaaaga cacagcttca gatgataaaa atttgtttat tttaagtaac
    13861 ataataccac cctttttaat tcgcagagtt cattatattt gaaaaaaaaa tatttgtcaa
    13921 ggtaatttat ttaaatggca ggctgccaga tgaccttcgc ttatatttgc taaaagtggc
    13981 tcttcaaccc tgcatatatc catgcaatga gggcatctgg gatggaaacg gcagccggaa
    14041 ggaattttta caggatttgg ggtttctccc ttcagtgcat ttttcgtcct gttctttaca
    14101 gccttagcca aaggcataac atctattaga gctttagtgt aaggatgttt gcatttgcct
    14161 tctatcaatt cgggtccgcc catttcaaca attttaccta aatacataat ggcaattctg
    14221 tccgatatta accatgccag ggaaaggtca tgggttataa atagaaatga tatgtctttt
    14281 ttatttctca attccatcag caaattaata attcctattc ttatggaaac atcaagcata
    14341 gataccggct cgtctgccac aatgaacccg gggttaagta taatggagcc tgcgattgct
    14401 actctctgtc tttctcctcc gcttaattca tggggatacc tgtacaggta ttcccctgcg
    14461 ggctttaatc ccgctaattc caatgcaact tcaactcttt cttttttatc cttttcgctt
    14521 tttgttatat tatgaatcga tagtggttcg gaaacaatat ctattacaga ctgtttagga
    14581 tttaaggact gatacggatc ctggaatatc atctgtgcat tctgcctgaa tatttttaat
    14641 tctttttcac ttttcgcact gatatcctca ttgttaaata aaactttgcc tgatgtgggt
    14701 tttaccaggt ttaatactgc tttccccacg gttgtttttc cgcttccgct ttctcctgcc
    14761 agtgaaagta tttctccttt tttaatagag aaactcactc cgtcaactgc ttttacaacg
    14821 acttggtctt ttgaaaaaat atcttctata aagcctcttt ttatattaaa atgtaccttc
    14881 aggtcttgta catcaagtaa tttatccatt ccacacacct ccgtaatgac aggcacaata
    14941 gtgacccggg cttgcttctt tcagttttgg ctcttcaatt ctgcatttgt ccgatgcaaa
    15001 actgcatctg ggattaaacc ggcagcccgg cagatggtcg gacaaatttg gagccctgcc
    15061 cataagggct tcctctaaaa tcctttctcc ctgtatatcc gggaaggatt taaaaagctt
    15121 ataggtgtac ggatgtagat gttcctcgta tatttgttct gttcttccct tttcaactat
    15181 ttttcctgca tacattatta ctatttcgtc gcatacttct cctaaaatgg ataaatcatg
    15241 ggttataatc aataaacctg tatttaattc ctccttaatt ttgttaagga ggtttaatat
    15301 ctgggcctgt acaataacat ccaaggcagt agtcggttca tcccctatga taagcttggg
    15361 atcgcatgcc agtgccatag caatcattac ccgctgcttc atgcctccgc tgaattcatg
    15421 agggtaatta tctattctgt ttttgtcgat ttccactaaa tcaaatagtt cctttgtctt
    15481 tttcctggct tcctcatagg ttaagtgatt atgatacatg attgcctcaa tcacctggtc
    15541 gccgaccttc atgacagggt tgagggcatt cattgcactc tggaaaacta tggagacatt
    15601 tttccatctc tgctttctta ccctatcctt gcttaatttg gttaagtcct tcccgtctaa
    15661 aagaatactg ccctctgtta tttttccgtt ttcgggcagc aaatttacaa tagataaggc
    15721 tgtggtggtt tttccgcagc ctgactctcc aacaagccct accgcctgtc ctctctcaat
    15781 agaaaaactt acaccatcca cagctctgac aatacctttg ctagtcttaa aatatgtttt
    15841 caaattatct atcttcaaca attccatcat actaccttct tttcagctta ggatttaaaa
    15901 tttcatcaag agcatatccc aaaaaagtaa aggacaaaac caataataca acgcatatgc
    15961 cgggaggcag aaagtaccag taggcaccta agcttgcagc gccggactca aatgcataat
    16021 gaagcatcat cccccaactg gaccttgaaa catcacccaa gcccaaaaaa cttagggtgg
    16081 tttcagacag aatagcttct gcggatacta aaatcgtatt ggcaaataca agggggaaaa
    16141 cattaggcaa aatatgcttt accatgatat ggcctgtatc ggcccctata gctgttattc
    16201 tttctataaa aggcctttcc ctgatagata aagtctgggc gcgcactatc cttgccgttc
    16261 ccggccagct tgtcatgccg attacaaata ttatattcca tatgctggtc cccaggatag
    16321 ctgtcagtac cagcatcaag ggcagccaag gtataaccag aaagaaattc gtgacctgca
    16381 tgattatcgt gctgattata ttatcataat aaccggcata aatacccaga acagtaccta
    16441 aaatcataga aatcaatgtg gacaaaaggc cgaccaggag ggataccctt gtgcctgcta
    16501 taagattgcc cagcacatcc cttcccatgt catcggttcc cagtaaatgg cttctgtccg
    16561 gaggattcat cacatccccg gcagttccga attcttgcag gtcaaagggg tagacaagag
    16621 accccatcaa tgcaaccgtt atgaaaactc caagtatgac agctcctgca actgccattt
    16681 tattatgaat aaatatgttg aaaaactctt tatatccctt ataaacaatc tcatgttccg
    16741 aatttttcat attatcagct cctgcttttc accctcgggt caagatatcc atatgttaaa
    16801 tccgccaata gatttgctac aattacagaa atagttataa gcagaaatat tccctgcaaa
    16861 agcggataat ccctgctttt gattgcgtca tacataagcc ttcctattcc cggccaggaa
    16921 aaaatggttt ctatttgtat tgcaccggct atcaccagac ccagattaat agcaatgatg
    16981 gtaatcatgg gcagcatggc atttggaaca gcatgttttt taataacata atcatcttta
    17041 aatccttttg catatgccgt agtaatataa tcttcagtaa ggacatccac aagtgtgctt
    17101 cgcatcacca gtgcatattg gccgattaaa agaaggctca tggttaagac cggcaaagcc
    17161 atatgcttta taacatccga tgatttttca ataacatctg cataggacaa tccaggcgtc
    17221 atcattccgg aagtgggaaa aatgcgaaga ttaactgaaa aaatcgtcac aaatactatt
    17281 cctatccaaa atgtaggcat tgcgtagcta attaaagaaa aactcaatga tacaatatca
    17341 attttagttc ctcttttcca tgcagatata acaccgataa aaattcccac tataatagca
    17401 agtatttctg cagcccctat cattaacagg gtcggcagaa ttctattacc tattacctgg
    17461 gcaaccggct gtcccttata caaaaaagat gttcctaaat caccttgaaa gaggcttttt
    17521 atgtaaataa aaaactgtat ataggaaggc ttgtctaagc caaacatctc cctggttttt
    17581 tcaattgcct ctgctgatgc gttgggattt ctcataagaa tttgcagagg attgccgggc
    17641 ataatcctga aaagaaaata attgaaaact aatactgcaa aaatagttat cagcgaatat
    17701 ataatttttt tcaaaacata ttgcttattc atattaagca tctccgattc aatatagggc
    17761 aggttgcacc cgccctatat ttaatttatt ttaatcgtcg tcttcttccg tgtttttttt
    17821 cttacgcatc attaatactg ctgctccggc aactaataat accacggctc caactattaa
    17881 tgctgtattt gaagagccgg attccccctg attctgtgtt ccttcaggca ctgtaccttc
    17941 agtttgtgct ttgggtttaa cattaacata gtttgtatat gtcaaattat agaaataaga
    18001 tccgtcttcc ggtactcttt tccatccttc aaatttgtca gtcctcacag cctgcaaatt
    18061 attatcgtaa aagaggatga tatacggagc atcatcatac agtatcttct gcatctggta
    18121 tactgtctca cgccgctggc tgtcatccat aattgtttgc tgttccagca gcaatctgtc
    18181 gtattcttca tttgaataat tacaatcgct caggttgcca atttccctcg tactcattac
    18241 gttaagaatt acagtaggat ctaaagcagt tccccatccc cagataaaca tatcaaaatc
    18301 ctgtgaataa atcttatcaa tcaaaacccc gtcatcaact gtttcaattt tcagttctat
    18361 gcctgctttc tccacatttg ctgcaatcat ttgaccagcc tttatttcct caggggtttc
    18421 tgaccttagg gtgaaaacaa aacttagctt gtttcctttt tcatcttctc ttatgccatc
    18481 gttgtcagta tccttataac ctgctttctc caataattca tttgcttttt caatattaaa
    18541 gcttcttaat tcgtccttgg gttcataatg ataataatcg cctagaggga ctagtgaagt
    18601 gcctgttttc ccctggccgt agtatacgac atccagaatg ttctgcttgt caattgccca
    18661 ttctacagct tgccttatat tcttgtccaa aagaagtttg tttccttttg aagaagaatc
    18721 ttccgagcag ttaaagctta attccgtaaa accctgtgcc acagcggata tggcttcaat
    18781 ttccggaaca ccttttaatt cttcaagctg cgatgcactg aaatttacta ctgcgtctat
    18841 ttctcctatt tttaaggctt gtgtcataac atcatcgttg gcatataata caaaaattat
    18901 ttcgtctagt cccggacttc ctttaaagta gtcttcgttt ttaaccagtt ttaggaatcc
    18961 tcccaggttc aattcttcaa atttaaaagg tccgctgcca acagggctgt cgtttgacca
    19021 tgtttccagg tcttccatgg tgagtgtttc caatatatgc ttaggcaaaa tcggggcatt
    19081 ggaaaggagc atgttagctt ttggttcatt tgtcttgatt accactgtct tctcatcagc
    19141 tgcctctatg gaactgatac cttccaatcc gcctgcatac atgcccaagc cgctggaaat
    19201 aatgtattcg tatgtatatt ttacatcttc agctgtcaca ttcttgccat catgccattt
    19261 tgcattttca tccaaaacaa aggtccacgt aagattatcg ctgcttttgc tccaggattc
    19321 tgcaagcgac ggtacaggcg ccatgttttc atccactctt accaaggggt cataaatcaa
    19381 catgaatgtt tcatctccgg taatcgacca tgataaaaag gggcttaaac tgtctatctc
    19441 ctgtgtgact ccaattctta aactgtttgt ttcatctgcc gcaagggtaa tgtttgcagt
    19501 gcctgatatt aacatagctg aaattaaaac aaaaataata attttctttt tcattggata
    19561 tccctccatt tttttaatat atatgctctg tcatttcgct tatgcatatc tataaaactg
    19621 cagcttcttc tatggaaaat attttgtcat ctgcatttat gtatgcctta actcctatag
    19681 gcagagtagc taaatctttt gtatgtccta tgggcaatcc gtgcaatacc ggtttgccta
    19741 gaggttttag atagaattct agaatatcct ccaaactgat atctgagtga aaccccggat
    19801 ctaatctcct tggcacacag cgggcacatt cacctatcac aaatccggat gctttttgca
    19861 ggacgcctgc atttgaaaga tggcacatca tatgatcaaa tatccaaggt tcagtatcaa
    19921 cttcctcaaa gaacagtatc ttgtcctttg tatcaatttc atagggagtg cctaaagaag
    19981 aacatataag tgttaaattt cctcctatca gttccccctc tgcctggccg ccgtttatta
    20041 cgtttatcca ttttttcctg tctgccagat ttatttgtcc aattggagct tgtccgtcca
    20101 atgctttaaa caaatgctcc ttagtgtaat ccgttaattc ttcatcgtta aatctggtca
    20161 ttcccggtcc atgaaaggtt acaataccgg taattttatt tattgccaga agcagggatg
    20221 taatatcgct aaagcctaaa aaaattttag gattttttct tatcaggtcg aaatctaaat
    20281 atttcaggac ttgtgccgaa ccatatcctc cctgtgtgac aaatattgca tcaatgtctt
    20341 ttctttcaaa catttcgttt aaatcttcgg ctctttcaat atcccgtccg gagaaaaaac
    20401 ctgtcttctt attcagattt tttgacggtt caaccttaaa tccccattcc ttcagggttt
    20461 ttatccctct atctatatca cttttgtaat aagaagggct tgccggtgat actatgccga
    20521 ttttatttcc aatctcaagc cttttaggtt ttattttcat ttttccccct ctttcataat
    20581 cctgtgatcc tgctttttaa tttatctgct tgctataata atccaaaaat ttttatagag
    20641 taacttatta tataaagcag tcaaaaaata gtcaagatat aaggtgtttc cctgttataa
    20701 cagttttaac caaggataaa agcgcctata ctgttaatta cagtttaagc gcttgtttaa
    20761 ggattcttta gttttatttg cttgtcagct tctttgtatc tctggcaatc attacttcct
    20821 catttgtagg aataacaaat acttttactt tagagccgtc ttttgatatt tcagcttctt
    20881 tccctcttgt gttgttcttt tcatcatcta tttcaattcc taggaattca aggccctcgc
    20941 atacttgttt tcttgcaggc ggagaatttt cgccaagacc tgcagtgaat accaaataat
    21001 caagtcctcc cattgctgct gcataagaac caatatactt tctcactgta tatgcatatt
    21061 tatccaatgc cagctgagct ctagtgtttc cttttccagc ttctttttca atgtccctga
    21121 aatcgttgga aacgccggaa atacccaaaa ctccggattt tttattcatt aaattgtcaa
    21181 cctcttcacc tgtcatgttt ttcttgttta ttaaaaatgt tacaattgcg gggtctatat
    21241 ctccgcatct tgttcccatt accagacctt caagaggagt cattcccatg cttgtgtcaa
    21301 aagactttcc atccttgatt gcagtcaggc tagctccgtt tcccaggtgg catgtaacaa
    21361 ttttggttcc ttccaggctt ttgcctgtta aatcagcaac cctttctgaa acatactgat
    21421 gtgatgttcc gtggaagccg tatcttctta ttttcaattc ttcgtacaat tcgtagggca
    21481 atggatatat gaaggataca ggcggcattg tctgatgaaa tgcagtatca aatactgcta
    21541 ccatgggtac gtccggcatt aattccttgc atgcctcaat tcctgtgatg tttggaggat
    21601 tgtgaagagg tgccagactg atgcactctt tcatggtatc aattacttca tcgtctatta
    21661 atgctgaatt cgcaaatttt tctgccccgt gaaccactct gtggcctaca gcatcaattt
    21721 catccattga tctgattgcg ccgtattttt catcaagcag tgctttcagc ataagattga
    21781 ctgcttcctt gtgatttttc aagtcttctt caataaccag cttgtcaaat cccggttttt
    21841 cgtgttttaa taaacctcca ctaattccta tcctttcaac taagcctttt gccaggacct
    21901 tttcaccatc catatttatc agttgatatt taagtgatga agacccgcag ttaattacta
    21961 gtacattcat ataattcctc catagttttt ttatatataa aatccttaga ttttaagtac
    22021 agctccttat tatgcctgtt aatttcttat tgtccagttc aaagatatcg cagtttgata
    22081 ttttataatt ccataatgtg tcatcatcgg ctatattata atacaaaaga acagcctgta
    22141 tcacaccgta atgagttgat atcagcgaat tgtcaggcac cttatccata aagcccgcaa
    22201 ctctttttac aatatcatca tatccttccc ctttggggaa tttatattta tgtttaaatt
    22261 cagtccagct ttctacttca tcggcataca tctctacaat ttcttcccat gtaaggtttt
    22321 caaaaatacc gaaatcaact tctgttatac tgttttcaat tattatgtct tttctgtcaa
    22381 acccgatttc atctgcaatt atttcagctg tatatattgc tcttttcaaa gggcttgaaa
    22441 aaatcttatg tattgttttg tccttcagct tttttcctgc cttcactgcc tgcaaaagac
    22501 ctttttcagt cagttcaaca tcggttattc cgctgaatct ccttgatata tttgcttctg
    22561 attctccgtg gcgaactaag tatatcataa ctgcctcatt aagaaaagta ctgttatcat
    22621 aaaaattatc tggcttattt cagttataaa cccgcatgta tcccctgtca tacctcctat
    22681 tttatcttct atcaacgtgg atataaaata tgaaaggctg actgctgcat aagatgctgt
    22741 aaatattatt gttctttgcc atgacaggat tgaaaataca aataatatgg ataaattaat
    22801 caatagaacg ctcttgtcaa tattttcaac aaacgcagtt cccataccct ttgtcccttt
    22861 tgcgtatttt ttattccagc agccggctat tgctcctgcc cgtcctgcaa gaggcataat
    22921 aaatactgct tcaatgctta tgtaagaaaa cagaataaat tgggaaagga caattaaaat
    22981 caatgttatt actccaaaac ttcctatgcg ggaatcactc attatttcaa aaacttcatc
    23041 ccctgtcctt cctgaaaaca gaccgtctga agtatcggca gcaccgtcca aatgtattcc
    23101 gcctgttata agaatataca taatggtaat tgccagtcct cttaggaagg gtatctgtct
    23161 gttaaaaatt aagtatgtca aggctaaaag aaaaccaata accgctcctg taagggtgaa
    23221 aagattcaat gctcttataa agttttcttc gttgtattca atttttcttc caacttgaat
    23281 tcgggtaaaa aacgaaatga gagtcaaaaa tcctgaggcc attttaccat ttcccctttg
    23341 tctttaagct ttattggtat tcccgaaaat ataaaataca tattgtccga tttaaggcca
    23401 agcctgctgt tgatacgtcc gcttatatcc ctgaaataat ttcccatata gtaagaaggt
    23461 acaagaccca tacctgtttc attagatact attattagtt ttttattgtt gttattgcat
    23521 acatcaatta atgaaaaaac ctcacttgta attttctctt ccaggatatt aacatcttca
    23581 acactgcagg tgtcaaaatc caaactgctg tcaaccatgt aattagcaat cattgttgta
    23641 atgcagtcaa acagtattac ttgagattga acaaataaag gattatcaat cagcttgtca
    23701 aagctgccgt acgcctccag cgtgtgccat gaggcaggtc tctgctccct gtgtctttta
    23761 actctgtcag ccattgcctg gtctgttaca actgcagatg ctatatacaa tacatgctgc
    23821 gaatattcct ttgcctttgc ttcagcaaat gtactctttc cgcttctcgc accgccgata
    23881 acaaatgtta agctcataat atcaccattt gcatttttct tctgatataa aacagtatat
    23941 catcaattta acaaaaaata aacaatgatt ttcaagaatt ttataacaag ggaaagggag
    24001 tactgtcata ccataacaaa aagagcccgg gctctttttg atttgataaa ctttttaatt
    24061 tggttaaaac agagtgttga attctttctc atcagttttg ttcaattgat ttgacttttg
    24121 ctgtacaggc ggtatttgtc cttgaacttg actaatgccc atttttttct tagcttcatt
    24181 taattcgaac tgtttaacat tgttggaaaa attataattt tgactgctgt tggaattctg
    24241 cattttttta tcatcctttt taaatgtttt tagctttttt agctgataat attatctgca
    24301 tataataatt ttatatagct gaaaaaattt aagtcttttg taaaaatttt tatttaaatt
    24361 ttactgtctt gaaattattt gacatcaagc ctgtttataa ttaagatatt attataaaat
    24421 gcaaatatgt gacaagatga cagcacccct gtcccttgtc atataagacg gggataaaaa
    24481 tatgaaaatt accggtgttg ttgctgaata taatcctttt catgcaggcc acgaatacca
    24541 gcttaaaatg gccagaaaac tatcagactg cgattgtatt gctgttgtta tgagcggtaa
    24601 tttcgtccag cgcggagaac ctgcaatcat agataaattc aatcgtgcgg aagctgcagt
    24661 atatggaggt gccgatattg ttattgagct ccccatgcct ttttcatgcc agaatgctga
    24721 aatgttcgct tttgccgcaa ttaaggaatt aaaaaaactt aatgttgatt ccataagctt
    24781 cggctgtgaa aatgacgatg tcaagctgtt ggaaaaaatt gcatgtctgc aattaacgcc
    24841 ggaattcaat aaaaatttta aggaagaaat aaaaaaagga ctgtcctacc caaatgccat
    24901 gtcaaatgca ctggtgtctt taattaatga tgaagctgaa gctgtttatt cgcccaataa
    24961 tgtcctggct gtggaatata taaaaagcgc catgaagtta gatttaaacc ttagctacta
    25021 tcctgtcaaa cgtttgggca tggctcacaa tgatacagaa tttcaaggac cttttcacag
    25081 tgcagctgcc atcagaaact ccatgctgga aggctctttg aataatctta agcttacgga
    25141 aaaaagtctt gagctgattg aaaaattcta caatgaccac aaacaattca acacactgaa
    25201 caattatacc gattatttat attataaaat aattacatgt gatgacttaa atacaattta
    25261 tgaaataaag gaaggattaa acaataaaat catgtcgcag tcattcaagc atgaaaatat
    25321 tgatgacctt ataatgtctc ttaaatcaaa gcggtataca tattcaaagc ttagaagatg
    25381 tcttcttaat attctccttg acataaaaaa ggttgacata attaaaatta tgttaaccaa
    25441 taacgattat gttaaagtgc ttgcttttaa taatgcaggc agaagagcta taaagcatgc
    25501 aaagaacaat ggaattaagg tgattaacag gttttcagac tataaaaaat ataacctgtc
    25561 tcctgaaaac cttaaggttt ttaatataac aaacaaatct tcaaatattt attaccttcc
    25621 tttgaaaaac agaaaaataa acagcgaata tactgaaaat gcagtgtata taaatattta
    25681 aataatgaaa ccgccttttc cctttcaatc tttttagtat gaaagaatct tccctatagg
    25741 ctattttaat aatcttataa gttgttatac cgatcagata aatttatggt acaacaatgc
    25801 cgatattttt cttctcctgc ttttccttga gcaaaactct tatctccttc acacttattt
    25861 tattatgatt aagcttaatt tgtcattcat tttaataata atttccgaaa tgtttttatt
    25921 tctggctcaa cataattgcc tttataaaac ataatgaaat tattgtatat ggaaaagcat
    25981 ataaacaatt tatggacaat aaaataaaag ggggtgtttt ttatgttaaa aagaaatttt
    26041 attcctgttt ccataatgat gattattttc ctgttcgtta tgaaaccgga cttagtttcg
    26101 gaaggtgttt ttaacggctt gaaaatttgg gttaacaata tgattcccta cctgcttcct
    26161 atggccattc tatcaaatat attgctgcaa tataatttct tgtacagatt gtcagaaaag
    26221 ctatccttta tatcaaatat aatatttcag agcagatatg cattcatccc ctactttatc
    26281 agcttcgtgg taggctaccc ttccggagca atgacagtga ataccatggc ttcctataaa
    26341 agaatagaca gcagtgaagc aaatgctttg gttgttttta ctaacaactg cagctttcaa
    26401 tttatggcag gcgcagtatc atattcaatg cttggagatt taagcctgtc caaatatatt
    26461 gcccttcccc atttgctcag tgcgttactc ataggaatgc tcataaaaaa agacagaccg
    26521 actgcagcct ttgcaaagga taaaaagcaa aaatatatat ccttttacga cgcctttaat
    26581 tcttcaattt ataaatcaat tacttccatc ttgtccatcg gcggtgttat tgtaatgttt
    26641 tcagtgtttt ccaacttttt cgataacctt cttatctctc tttcaaagta tcttttatta
    26701 agcagacccc ttagcgatat aatacattct gtttccgtag gcatcctgga aatttcaaac
    26761 ggctgcagcc tggctgcctc ctcctccctc cccttggatg ttaagcttat agttattaat
    26821 ttcctcataa gcttctccgg aatttctgta attttacaaa caactgcggt aacagatgat
    26881 tttaatttta atatcagctc ttatgtcttt tataaagcct tgcagggatt attgtctgtt
    26941 ataatttgta tagggatgtt gatactgtta tgaaaaatac tgaatgattg ccgccccccg
    27001 cattatgtca taatatattt attgaaaaat acagcattat tgtgtataat tgaatcactt
    27061 aagaaaaaaa ggtgattata tgataaacgt tactttaatc ggtacaggag gaatggtccc
    27121 cctgcctaac aggtacttgg catcatgtca tattgaatgc aatggaaaat caatccttat
    27181 agactgcggc gaaggaacac aaatttcact ccacaaggga aaaataagtt taaataaaat
    27241 tgacacaata ttaataaccc actgccatgc agaccatgtg acaggattgc cggggcttct
    27301 tttaaccatg ggaaatcacg gcagaactga gcccctagat ataattgcac ccagaggaag
    27361 cacaagaata cttaattctc tgttggttgt ctgcggattt ctgccatatg aagtcaggat
    27421 tgcggagctc cacgacacta aaccccagga atttataaag ataggcttat atataacgtc
    27481 tattccttta aagcatcata taaactgttt aggctacagc tttgagcttc gtttaaaagc
    27541 tcgttttcag ccggataagg caaaagatct taatattccg gttaatcttt ggagacagct
    27601 ccacgaaggc ggggaagttg ttgtagaagg caggacgatt acaccaagta tggttctggg
    27661 aagtcccagg aagtccataa aggtttccta tgtaacggat acaagacctg ttaaatttat
    27721 tgatgaaata gtcagggatt ctgatttgtt tatttgcgaa ggaatgtacg gaaatgatga
    27781 aatgcttgaa ggtgctgtgg caaaaatgca catgattttt tcagaagctg cttatattgc
    27841 actaaatgca aaggttcagg agctatggct tacacatttc agtccttcca tgccgaatcc
    27901 ggaagaatac ctgaaatttg cctcaaaaat atttcctgac actcttatag gaagtgattt
    27961 aatgagcaaa actttaaaat ccctgtgacc ttgtatagca aagggacaca aaggacatgt
    28021 tcattataga aaatttacat aattaaagga gcggaaatcc gctccttata ttgtttataa
    28081 aattgcttta gaaaatacct aatccgaaga agtcatcaag ctgacccatc aataccaata
    28141 ccatggcaac aggaactata aatttgaagc aaatatccca gaagccgtat ctcttcatct
    28201 taatgcctgg agaagcttca acctcttcct tgataacagt tgtcttcagt ttccagccga
    28261 ttatcagtga catcaacaag gcaccaagag gcatgaacac accttcagaa atcatgtcat
    28321 agaagtcaag ccagcagtca aatgcaactc taacttcctt cattccgaat atagtagccg
    28381 gtatgtcaat cattgcaccc cctgccttgc ctgcgccaag tccgtccatt gctacaggga
    28441 atcccaggaa gaatattaca agacccagaa taagggtgta ttttttacga ttcggctctt
    28501 cccccttttc aactgctttg tcaataaaga atgctgtaca aacctccaag agggaaatag
    28561 acgatgttac ggcagcaata aacaccagga aatagaaaaa ggcgccaaag aaattaccca
    28621 tgaatccgcc catgcctaca ctgaataccc tgtgaagagt aacaaataac agtccaggac
    28681 ctgctccgaa agaaacacct tcaactccct ggtacatgaa tgcaccaact gcgggcagta
    28741 cagccaatgc tgatagaact gcaaatgatg tgtctgctaa aggaataagc atggcattcc
    28801 attccaggtt ttcttttttg cttaagtagg agccgtaagt tatcatgcag cccataccaa
    28861 gcgacaggga gaagaacatc tgtccggctg caattttaag caccctcata agacctactt
    28921 cctttataat atccaagttt ggtttgaaca taaattcaag acctacaaaa gcaccttcca
    28981 gagtaacgct tcttataact acgattataa ccattacaaa caatgtaggc attgccactg
    29041 ctgtaaactt ttctataccg ccttgaacac cgcccattac tatgagaatc gtagcaccca
    29101 tgaaaattgc atggaacaat atactcatgg gagcactaag gattaagctg ccaaagaatc
    29161 caccgctgtc caatcctcca aagcctgcgc ctgcactgaa catttccatt aagaatccaa
    29221 tacaatacct gattgtaaag ccgcctaata cgctgtaaaa tgctaaaatc aaaaatcctg
    29281 acagccagcc cagccagcct atccatgtgt acttttcgct gattttccta tatgttccga
    29341 taactcccat gccatactta cggccaaggg taagttctcc cagcatagtt gcaaaaccaa
    29401 caaaagacac taataataaa taaattatta aaaatatgaa accgccgttt gcacccatct
    29461 tatatgggaa tccccaaatg ttacccaagc ctatagccga ccctatagct gccatcaaga
    29521 aaccaaaatt agtaccccac tgacctcttt tttcctgttc catttactat accttccctt
    29581 cttttttact aaatattata aaataataaa aaatagctaa aatcaaattt aattaaaatt
    29641 tttaggttta tttattcacc tccctgcttg aaatccagtc aatattttta agtagtctct
    29701 ctttgttttt ttatttttct ctcatctcct tattttataa attagtacaa cgttttctag
    29761 tttgttctat ccttaaaaac aatatacaca attattatac tcctcaagcc cgcttttgta
    29821 aagcaaaaaa tatattccaa taattattaa cattaagtaa actttatact atatttatga
    29881 ctataaattt tgtttatgct ttaacatgta caacgtttta ttttcactgc tgctgtcact
    29941 taatattatt tcaactgcct gccatgaggt gagcatatta aaagcagaat gtctttatta
    30001 tttactattt ttcatttatt gacagcatga ggttgagaat gattcctgtt acagctgctg
    30061 ttgcaatggc gggcaattca ataccgaaca taggaatcat gccgccttca aatccaaagc
    30121 ttccgccaag acctacaatt aggattactg ctatcactgc caaattttta gagctgaaca
    30181 tatcaacttt cctatcaatc ataattgtta taccctgggc tcctataact ccgaataaat
    30241 atattgacaa tccccctatt actgctgttg gaatggaata tactatgttt atcaatggtg
    30301 tgaagcagga aataatcata gtaaacacag ctgccagcat aagaacatat atggagaagt
    30361 ttttcgatat ggccatggcg ctgatatttt caccatagtt tgttcctgcg gggcccccta
    30421 taaaacctga aataatatct cccattccat ctcctataag atttaatccc aatttatttt
    30481 caatatcata ttttttatta atgccttttt tcttagccag gtcattaaca tatatgtcca
    30541 gttgataaac atgtgccgtg gattcaggaa ttgttgctat ggcaatgggc ataattgcta
    30601 ctacagctgc cagggtaggt tttgggaatg tgaaaatagg aagatttaat attcctgtag
    30661 tttgaattgt gtcaaaggat acgaatggaa tgccggtcac tcctcctatt gttaaagcag
    30721 cggcatatcc tgtgagcaag ccgaacaaaa tcggcagctg gcttagtttt ccctttaaat
    30781 aaacagaata caaaattgtt gagaataagg taataattgc aatagtccag gcccagctgc
    30841 ctgcattagg attacctagt gctgcttcag gaaatgctgc agcatttgtc attgcattgg
    30901 ctgccaatga cagtcctatg attatggcta tgcttcctgt tacggtcgca ggaagaactt
    30961 tttcgatttt atccttgccg gatttattta ttatataacc tgccaatatt gaaacaaaac
    31021 ctgacatcac aataccgaac tgggcaactg aaatcttatc gttcagagag taagacgcaa
    31081 atgcttctgc actcatgatt gatgcaattg ctgctatata ggaaaagctt gacccatagt
    31141 aaagtggaat ttgtcttccg gtgataagga taaatcccaa tgttgccaaa ccgctggcaa
    31201 aaattgttgt agaaacatgg aaacctgtga tgagagcaac caagacagtt gcaggaaaca
    31261 ttaccactat ttgctggagt gcgaagaaaa ttagaccaag cagcggtgga cgctcgtccg
    31321 gcaaatagcc gaatacttca ttttttgttg acataaatac ctccatgtaa ttaataaaaa
    31381 tccccggccg gatggtcaag gattattata cacaataaat caaatggata ttaaatcttg
    31441 acggcatcac ggtaccgcgt taaagattat tttctaaaat tatagcatgt ataaaccggg
    31501 cagtcaacaa aaaattatgt agactgcccg gttaggtgat ttgtttgctg ccttcttagc
    31561 tggggccctt tttgcctctt tttttataat cttcatcaat tttggatttc cagcttgagt
    31621 ccaccttttt acccatccgc atctcactta caaaagtgct tacagcgctg taatttaaat
    31681 taataccctg gccgtctgca caaaagtttg atgccctgtc ttcatcgatg ccaaaatctg
    31741 ctgctgtttt taccgcatca atatttgcac cgagaaatac aaattcccag gaatattttg
    31801 ttttctgatg ttcaaccatt ctttttacct tcgcttttga aaattcatgg ctggaatttt
    31861 ccataccgtc ggttatgatt acaaatatta ccttgccagg gtggtcatct tcccctgtgt
    31921 ctgaaattga ttttccaata tagttgatag ttctacccat ggcatccaaa agtgctgtgg
    31981 agcctcttac aaagtattct tgttcactta tttcatccac attcttgatt ttttccctgt
    32041 catgaagcat ttcataatca tgatcaaaga gtacagttgt aacaagagca tcccccggct
    32101 cgcttttctg ctttgcaagc attgaattga atccgcctat ggtatctttt tcaaggcctg
    32161 acattgagcc gcttctgtca agaataaaca ctaattccgt taaatctttt ttcatattaa
    32221 ccctccattc ttattgaaca aaggataaca tttatttaaa atattatggt cgctttaaat
    32281 gcgacaaatt ttaaagacct aaaagaggct gttcaaaagc aaacaaagct tcattaatct
    32341 caaaaatatt atagattttt tcttccaaaa agaactcaac tattaaatca aacttactgc
    32401 tgcgggacag ggcataacct gctttttcga gcaaatccct tgtttcgtcc acatttaatt
    32461 ccagagccac tgcgaatgcc aatgctgtat ttttcctggg tttgtaattt ttatccgtcc
    32521 gtatttttga aaaaagcttt ctgtcaatat tggccttctt gtatgtttcc acatcggtca
    32581 ttcctttttc gtcaatcagc cgaagaagca tttcagagaa tgtttcatcc agctgtttta
    32641 ccacatcctc caagcttctg gctttttgct ctttaacact ttttaaggat ggagccgccg
    32701 cataattgaa ttcatattcc tgcaatactc ttcctctgtc caaaagctta tggtcttcca
    32761 catacttgtc atcaatgtat tttcttattt tgctgaaaag cttctcactg aaggcaaaag
    32821 ctttggtgtc aaacataaca aggtagaccg tcatttcatt ttccagcaaa aattcgctga
    32881 ttgcagaaat tgcaaccttt agtgcttcat ccttggggta tccgtaaatc cccgaagaaa
    32941 tcaagggaaa agcaatggat tcaagtttat ggtcttttgc aagcttcatt gaatttaaat
    33001 aggcattttt taaaagctcc tcttcattgc tgtttcctcc attataaatg ggccccacag
    33061 tatgtatgat gtattcggca ggcagcttgt atcctttagt gatgacagct tctccggtat
    33121 tgcagccgcc tatcttatca cattcagcct gaaggtcttc tgcccctgcg gccctgaaaa
    33181 ttgctccgca cactcccccg cccatcttaa gggctgagtt ggcagcattt acaatagcat
    33241 ctgcctttac cctggttata tcatttctta ttatttcaaa cggcattgcc tatccccctt
    33301 tatcattctt tatctttatg atagttataa gttttctcac gtcataaaat gacgttcaga
    33361 ttttttatca taatcttatt ataatatctt caggatttat tatcaagggc tttgacttat
    33421 tgttgatttt tcactaatta tttcatataa taatattagt gtgtgcggag gggacctatg
    33481 gctcaattat ttgttgttct tattgttttt ttgttaattc ctttgttagg caggtttaaa
    33541 ctcaaattat cttatatact gttgatatca tgcggagttt tagctctttt ttcgggaatg
    33601 ggagctcagg ctgcatggca gtcggtgaca aatatcttta ctgatttttc atcgttaaac
    33661 actatacttt cagttatgat ggtgagtatt ttaggcggtt taatgaagaa ttacaaaatc
    33721 cttgacagaa tagtcagctc aatttatcag ataattcaaa acaaaaagat tatattgatg
    33781 attcttcctg ctttgatagg tatactgatc gttcccggag gagctctttt atccgccccc
    33841 tttatatatg aaatgggcga agaattg
//
LOCUS       DAOPUT010000011        32978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_1272_length_32978_cov_18.442274, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000011 DAOPUT010000000
VERSION     DAOPUT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 32978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32978
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_32978_cov_18.442274"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..694
                     /locus_tag="PKK75_02220"
     CDS             119..694
                     /locus_tag="PKK75_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNZ82443.1"
                     /translation="MSKLWFGLISLGLLFSFINKSPETVTNVLFFETEKSVELILNLI
                     PVMAFWTGLMRIVEKTNILNKLSTSLKPMVRYLFKDVQHDVKAVNAILMTLAANMLGI
                     GNSATAFGIKAMQEMQKSNANKKTATNAMCMFLIINVSSIQLIPLNIIKLRAASGSSA
                     PSEIMVPTLIVTAFSTMIALIFAKYYERKEL"
     gene            691..1206
                     /locus_tag="PKK75_02225"
     CDS             691..1206
                     /locus_tag="PKK75_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HNZ82444.1"
                     /translation="MNISDLILPLLIAAILFYAVSKGVDVYSAFVEGAIDGARSALKI
                     FPYILAIIFAINLFIQSGAEAFIVGILEPVTLFAGFPAELLSLSIVKPLSGGGSLGVF
                     QTILDNYGADSYIGRCASVIMGSSETIFYTTAVYFGSVGISDYRYTIKTGLLSHAATI
                     FAALAVCRLMF"
     gene            complement(1195..1788)
                     /locus_tag="PKK75_02230"
     CDS             complement(1195..1788)
                     /locus_tag="PKK75_02230"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HNZ82445.1"
                     /translation="MYFEIVFFSLLSIVTAIIFLKIKKRKFNFNRYKVRIRGYKVLIL
                     AAFIKIAAEFLFRIFTNVSLLRILSLNWVIYFGLLYVSLLNIKKSFMMLVFFGTLFNF
                     AAILANDFKMPVYVSELLTDVEAKKNFLLSGKDLVHSLLTEETRLKFLCDIFTLHPPY
                     PFPKTISVGDVFLLAGIFAFWQDLFYESSIRPKRSKH"
     gene            complement(1790..3088)
                     /locus_tag="PKK75_02235"
     CDS             complement(1790..3088)
                     /locus_tag="PKK75_02235"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNZ82446.1"
                     /translation="MNNIKQSKKRNIKLYAYIIFLALTSLMLLIYLFKNYTISDYTLL
                     IVFSVLSALAETFLIPLPKVGAVSVSFALTFSAILLTNPLTASLIAAFGMIFRCPYVD
                     GKGRVHMFNNPIYKTVFNVSQYIINAGAAGLTYLYLNRILNFGFYFFNPIAAVVCLFV
                     YLTLNSVFMASLMSILLNEKISYIWKNYLLGFINISLVGLLGIVVAFSYDNFGTTGIL
                     LFFIPLMFARYTFKLYLDMRKNYFDTLNVLVRAIEASDPYTSGHSMRVSAYAVAIANQ
                     VGLPQGKIDLIKSAALLHDIGKIGIDKSILNKNGKLEKEEFDKIKSHPEIGATIIADL
                     SYLTNISDIIRHHHERNDGKGYPDGLSHEDIPLETSILTVADSFDAMTTDRPYRGALS
                     LESALQEIKDNGGTQFNPDIVDDSIIALKKAFFKLAEERV"
     gene            complement(3300..3923)
                     /locus_tag="PKK75_02240"
     CDS             complement(3300..3923)
                     /locus_tag="PKK75_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82447.1"
                     /translation="MNFGEMEYFLNSIKSVISSKVIMNSNNEISEIHVLSDSSRQSKQ
                     IARDIRTALLSKFNTDVDYKIISVAQIDKDISFNSDFRLLYEGNTIETTCDRISIKTK
                     LSWDNKEFLGVSEGIKSEKQSLIVAARSALDAIKQAIGFDCFIVEDIQNVRIIGGEAL
                     VVAINQINNGKENILVGASVVDNNKIDSVIKATLNAINRKICLLIKE"
     gene            complement(4162..4884)
                     /locus_tag="PKK75_02245"
     CDS             complement(4162..4884)
                     /locus_tag="PKK75_02245"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNZ82448.1"
                     /translation="MENILKKINMVFAAAIIIIMLVSIFLNLTRTAETIDAAGEEKIE
                     KIKILIDPGHGGVDQGASGDMKVAEAPINLAISKKLMSFLEGSGFEVEMTRYDDSGLY
                     TELSGTIRAKKNEDLKNRVELINSSNADLVISIHLNSFPQKQYSGAHVFYQKSNEIST
                     KLAADAIQESMKNILDKNNSRVPQIKKGIKIMDDTSLPVILIECGFLSNNNEERKLIS
                     GDYQEKTAWAVYTGIITYFNEL"
     assembly_gap    5102..5201
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5202..6388)
                     /locus_tag="PKK75_02250"
     CDS             complement(<5202..6388)
                     /locus_tag="PKK75_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNZ82449.1"
                     /translation="MKVKSVLKVLGIVLFWEAVLMVPSLLISIIDNSYEIKAFVITIA
                     ATAVTGFILSRFEIDSAEMRKREGYAAVALSWLVMSIFGALPFYLSGSIPSYIDALFE
                     TVSGFTTTGSSIIRDVEIIPRGLLFWRSFTHWIGGMGVLVFTMALFPSIGGRPVFLMR
                     AESPGPSPGKLVPKIGESSKILYIIYAVMTVIVIILLIAAGMPVFNSFIYAFGTAGTG
                     GFSMDALSVGAYNNPWIDWIITIGMFLFGVNFTLYYLLIKKEFKQIFKNEEFRLYCFY
                     TGLAIALIVINVIPRFNYDFFEALRYSAFTVSTLVTTTGYATTDFNLWPMFSKSVLLA
                     LMFFGACAGSTAGGVKSVRILVLFKAFYYEIKHTIHPKSVQTIKVDGKSLDDDTLKSI
                     MIF"
     gene            complement(6422..7777)
                     /gene="trkA"
                     /locus_tag="PKK75_02255"
     CDS             complement(6422..7777)
                     /gene="trkA"
                     /locus_tag="PKK75_02255"
                     /inference="COORDINATES: protein motif:HMM:NF007033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNZ82450.1"
                     /translation="MKIIIVGGGKVGYTLAQQLSTEDHDITLVDNDANVLTHADETLD
                     IMCIKGNGASAEILRSAGVGETDIILAVTDSDELNMICCVIGKKLGAKHTVARVRNTD
                     YSDEFKMLREELELDMVINPEMEAAAEIAQMVQFPSAADVETFANNQIELVEFRVLGT
                     DPIINQKLMDLNRHLPSRVLFCTVMRDEEVFIPNGSFSFLPNDIIQVIGERISISRFF
                     KYLGRSSHKAKNVTIIGGSRTAIYLTWILEEMGLNVKIIELNKDRCLVLNDILTDALI
                     IHGDGTDQEVLDNENIEAADVIVALTDRDEENLISSLYAHQCGVPKVILKINRQNYFP
                     IVKKLGLDSIISPKFITATNILRYIRALNNTKGTAVEKLYRVLDNKAEVAEFTVKNSS
                     DLTDVKLQDLNLKNDVLIAAILRNKKIIIPNGNDVIKEHDKVIVVTKTGFIADLKEIL
                     A"
     gene            8208..8513
                     /locus_tag="PKK75_02260"
     CDS             8208..8513
                     /locus_tag="PKK75_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNZ82451.1"
                     /translation="MRIKEHPILKFNKNKKIKFVFNGQEMEGYEGETIAAALHAAGVK
                     VLGKSLFLHRPRGFYCAIGNCSSCLMTVNGVANVKTCVTDLEEGMMVETQEGKGVIV"
     gene            8510..9595
                     /locus_tag="PKK75_02265"
     CDS             8510..9595
                     /locus_tag="PKK75_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ82452.1"
                     /translation="MKHAEIVIAGGGPAGLCAGISAAKSGARVMIIDKNKNLGGQLVK
                     QTHMFFGSEKQYASTRGIDIPEILIKELDKYKEKVEIKTNSTVTGMYEDGMITVLEKE
                     VNYYKIKPKAVIVATGAYEKHLSFPNNDLPGIYGAGAVQTLMNVYGVKPGDRVLMAGA
                     GNIGLIVSYQLMQAGVEVKAILDAAPKIGGYLVHASKIRRMGVPILTSHTVKEAIGKE
                     FLEKAVVCKVDEKFNPIEGTEITFDVDVICISVGLAPLNELLAMRGCEMKYIPQLSGL
                     VPVRNENYETTVENIFTAGDASGVEEASAAMVEGFLAGLCAAAKIGYKPDDFTDRKND
                     YLKQLENLRSGPAGKHILAGIKEIGGK"
     gene            9595..10083
                     /locus_tag="PKK75_02270"
     CDS             9595..10083
                     /locus_tag="PKK75_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ82453.1"
                     /translation="MFEKTGIPSMEMIMEKFPSIERINKGAVAVIECYKEIPCNPCET
                     ACKFDAIKVGEDINNIPRLNSGNCTGCGICLSKCPGLAIMIVDGSKSETTVEVKIPYE
                     FLPLPKEGQIVKGLDREGKYITDVKVLKVLNPKSFDRTPVITIEADRKFLYEIRNIML
                     EV"
     gene            10085..10363
                     /locus_tag="PKK75_02275"
     CDS             10085..10363
                     /locus_tag="PKK75_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNZ82454.1"
                     /translation="MDENTIICRCSDVTLKEIRELINDGYVTFDEIKRITRAGMGPCQ
                     GKTCGYLIMREIANATGQNLKDIKFQTNRPPVVGVKLGLIAKEGKNEK"
     gene            10353..11501
                     /locus_tag="PKK75_02280"
     CDS             10353..11501
                     /locus_tag="PKK75_02280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNZ82455.1"
                     /translation="MKSKAEVVIIGGGISGCAIAYNLAQKGVKDVVVLEKEYICSGST
                     GRCGAGVRMQWGTEMNCRIAKKSIEFYEHANEILEYERDVEFKQKGYLLVADTDHEVD
                     QFRKNIEVQHSCCIPSTMLSLEEAREIVPYLNTDILKGAAYCSKDGFLNPFHTTDAFY
                     RAAKRLGAEFNTFTEAIGIKVEKGEVKGVQTNKGFISTNIVVNAANGYGKGICDMVGL
                     DIPTYSERHQILVTEPVEPMQDPMVMSFGMNFYIQQSPEGTFIMGRGDENEPRDLRVT
                     SSWQFIEEMAKTIDLVLPPISKLRVIRQWAGLYNMTPDKQPIYDEAENVKGFYIALGY
                     SGHGFMFGPITGVAISEMILKEKPTIDVSMLNLNRFKTGKLLLEPSVV"
     gene            11678..12766
                     /locus_tag="PKK75_02285"
     CDS             11678..12766
                     /locus_tag="PKK75_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNZ82456.1"
                     /translation="MREKYLNKLVDILKKEKIDAILIAPSEEMEFIMGHNTHLCERFQ
                     ALIIKNDGSYFYICNLLTQDEIQSVLGPDIKVYGWFDGDKFTDTVNKAFQEQGLIGKT
                     VAVNSTERAFIILQIMEAMDVKFINGKPFLEDMRIIKDEEELENLRMAARITDESYDE
                     LLKFIRPGIKEADIAEKMKEIFKEKGADEGFTMVCSGPNSSYPHYNDDQRVIQEKDII
                     ILDWGCKYKNMCADMSRTIFVGGITEEERKVYEIVRASQEAGEKAAVKGAYIPDVDKA
                     SRDIIQKSGYGRYFFTRLGHGIGYSVHEAPDIKAGNERNLEKGMVFSIEPGIYIAGKF
                     GMRIENIVAVTEDGNEVLNKATKEIMIV"
     gene            12858..13886
                     /locus_tag="PKK75_02290"
     CDS             12858..13886
                     /locus_tag="PKK75_02290"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNZ82457.1"
                     /translation="MDFKEQALELHKNNVVIDAHLDLAGEIYNRYLAGEKDVIKNHYL
                     DSFKKGGFNLIVSSLYIDEIFLPEMALRTALGQMRALIEDVESLDGEVILVKSRDDLN
                     KAVKENKIGIVISFEGLEPIGNDIGLLKIFYELGVRAAGLVWSRRNYVGDGCSFSPVE
                     EGQRGGLTRFGVNVVRKMEEMNMLIDVSHLNDEGFWDVVKFTNKPFIASHSNSRAAHG
                     MMRNLQDDQIKAIRDRNGVIGINAYTNILGVREGENSISRLADHIEYMVNLAGPRHVG
                     YGFDLCNGFYNSALKYKFEPRNSDGLSSHSEAVKLTEELLRRGISQEDCKLIIGGNFL
                     RVYSDILI"
     gene            13900..14433
                     /gene="lepB"
                     /locus_tag="PKK75_02295"
     CDS             13900..14433
                     /gene="lepB"
                     /locus_tag="PKK75_02295"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNZ82458.1"
                     /translation="MTDSNRQRIWYFIQEWVFPVIIALIIVLLLNKFVFILVTVPTDS
                     MEDTIIAGDRLYVNELFDINNAKRGDILVFKSDELEDKRLVKRLIGLPGETVEIKRDG
                     SIFVDGKKIEEPYALESHGEEKVFTVPLNSYFFLGDNRPISYDARYWNEPYIGEDKVI
                     GKVEFRFFPFNRIGEVE"
     gene            14570..>15612
                     /gene="pepD"
                     /locus_tag="PKK75_02300"
     CDS             14570..>15612
                     /gene="pepD"
                     /locus_tag="PKK75_02300"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HNZ82459.1"
                     /translation="MKRVLEGLKPEKVFYYFEEISKIPRCSGHEKQISDYLYNFARSK
                     NLEAIQDEYLNVIIKKPASRGYENAPAVMLQGHMDMVCEKNEGVDHDFSKDPIKLRIK
                     DGCIYASDTTLGADNGIAVAMALSVLDSDLEHPALEVLITTDEEKGMTGANKLDGSLF
                     KSKYLLNMDSEEEGVFTAGCAGGSEVDFKIPVKFQNIGGRAYKISVKGLSGGHSGVDI
                     HKEKGNANKILGRILYDLIDDTELFSIEGGSKTNAIPREANAVLTIKDLDKVSEKLDK
                     WNEILKNEYAFTDPNVKVTLTDLKEEVFPLDKEIYKKAATLINLIPVGVLSRSTAIDL
                     VISSNNLGVITTD"
     assembly_gap    15613..15712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15713..16158
                     /locus_tag="PKK75_02305"
     CDS             <15713..16158
                     /locus_tag="PKK75_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ82460.1"
                     /translation="AIDLVISSNNLGVITTDEKYIRLFNHPRSSVETLLTEGFIPAME
                     QLGDMLGVECERGSYYPGWEYAKESEIRDICIGVYRDMFKKEPLVGAIHAGLECGLLM
                     DKIPGLDAISMGPDAYDAHSPNEHVSISSVENIYNLLCEILKRIK"
     gene            16508..17434
                     /locus_tag="PKK75_02310"
     CDS             16508..17434
                     /locus_tag="PKK75_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82461.1"
                     /translation="MDSKLAIYIVKRMALAVVTIFLIITITFFAMHNIPGGPFLSEKA
                     ATPAVTKALEAKYGLDKPLSEQYTTYLKGVAKFDFGPSLKQRGREVSTVIMTGFKVSS
                     KLGGTAALIGLSLGMFFGSLAAVFHNKAIDKIIMVISTAMVAMPSFIIATLLLLIFCV
                     WTGIFPANGNEPGGLILPIISLSLYPMSYTVRLTRSSMLDVLSQDYIRTARAKGVKPS
                     KVLFKHALRNAVTPVITYAGPLIAYIITGSLVVEKIFAVPGLGKEFVSSIFNRDYTLI
                     MGTTIFLGALIIVMMLVTDIMYKVFDPRIQLG"
     gene            17450..18529
                     /locus_tag="PKK75_02315"
     CDS             17450..18529
                     /locus_tag="PKK75_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82462.1"
                     /translation="MNNINDLPKKNPLSFQLNLKDFEKATDEEKAQQVRMSESVTFFK
                     DGIRRLKRNRIAMACLWIIVAIAILVTIVPMVYPYTYEQQLGVAQGKRTDSSYNNLKP
                     FEYGETELKRIESGEKIFPHIFGTDASGRDYAIRVIFGARISLLVGFFAAIIVLIIGV
                     VYGSISGYFGGKTDLIMMRIVDIIYSLPDMLMIILLSVVLKEVLTPKIEGTSLAKIGT
                     DMLSIFIVFALLYWVSMARLVRGQLLSIKQNEYVLAARSIGASPWRIIKKHLLPNSMS
                     VIIISTALQIPSAIFTESFLSFLGLGVSIPMPSLGSLASDALSGIYSYPYRLIIPALF
                     IWIIVLSFNLLGDGLRDAFDPKLKK"
     gene            18540..19571
                     /locus_tag="PKK75_02320"
     CDS             18540..19571
                     /locus_tag="PKK75_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82463.1"
                     /translation="MSLLSVKDLHTSFFTSSGEVKAVNGVSFDLDEGKVLGIVGESGS
                     GKSVTAYSILQILSNPGRIVSGSIKLKGKELVGCSKEEMQKIRGNQISIIFQDPMTSL
                     NPVFTIGSQLMEAILLHTKRNREQAKERAIEMLKLVGVNEPERRINQYPHEFSGGMRQ
                     RVMIAMALACEPDILIADEPTTALDVTIQAQILDLMKDLQKQLGMAIILITHDLGVVA
                     EMCDEIIVMYAGEISERGTSDDIFYNPKHEYTKGLLKSIPTSQNEKMRLKPIGGNPVD
                     LLNLPDGCAFSPRCENAMKVCLYHNPKELKVGEGHASRCWMNIKSGIEENKLTKEEVE
                     EMYKHEQQQ"
     gene            19555..20526
                     /locus_tag="PKK75_02325"
     CDS             19555..20526
                     /locus_tag="PKK75_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ82464.1"
                     /translation="MNSNNNNLLEVKNLKQYFDISTGLLRSKPLKAVDDISFSIKKGE
                     TLGLVGESGCGKTTVGRSILQLYKPTGGEVIFKGKKIGSKESIRNLRQNAQMVFQDPY
                     SSLNPRMTVGDIVGEPLDVFKLYKNSKERNNKINELMDIVGLSSEHATRYAHEFSGGQ
                     RQRIGIARALAVQPEFIVCDEPVSALDVSIQAQIINKFEDLQDQLGLTYLFIAHDLLV
                     VKHISDRIAVMYLGRMMELADSDELYNHPLHPYTISLLSAVPVPDPNYARNHRRILLE
                     GDVPSPLNMPSGCPFRTRCQRATKECAETRPELREVSPGHMVACIHV"
     gene            20676..22529
                     /locus_tag="PKK75_02330"
     CDS             20676..22529
                     /locus_tag="PKK75_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ82465.1"
                     /translation="MKKILSLILVLAMVFSLAACGNNQPPAQPETPETPETPETPETP
                     EAPGIAESITVSVGAYPDTIDPALNSAVDGATYIIHTFAGLVGYRQKADGSLELYADL
                     AKELPEAQPTDDGKVKYVYELKDGLKWNDGSDLTAQDFVYAWNRAADPITGADYNYMF
                     EVVDGYAEVSESDDEGNRVNPDAKLNVTASEDGKTLTVVLPIDVPYFNELAAFPTYMP
                     VKQSVVEGNEAWATAAETYIGNGPYKVVEFSQSELIMKKNEYYHDKDSIKTEELVFAF
                     NDDDTSILANFQNGSYMFIDSVPNDEIKSLQASNPDEFFIEGQLGTYYISHNVNDTAF
                     AKFGEADKAKIRKALGLMIDRTYIVEEIGQAGQVPAAGFVAMGLLEPDGATEYIAVNG
                     PNRDGKGYYSVDPNDYENNSKEAIALLKDAAESSKLFTVDDAGKVSSEFPTVAYITNV
                     GTGHERIAEYLQATFASYGINMTIEKQEWGTFLQTRKQGNYSVARNGWLGDYNDPISF
                     LDMWITTSGNNDSQFGKGEHANYAGYTYKGEGGKTWAESYDKIISEVKASKDAVERFK
                     LMHDAEDILMSTGAITPIYYYTDIFMMSKDLDGAFASPLGYKYFMYSSVAE"
     gene            complement(22570..23931)
                     /locus_tag="PKK75_02335"
     CDS             complement(22570..23931)
                     /locus_tag="PKK75_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ82466.1"
                     /translation="MNNMKILIAGNDKLYITSLSSFVKKAALDYSYCLGAENAYKELD
                     NAEYSFFIADYELFTNEFSIRSFTSSFPLTDVIITSSAPSYRQESKSASQGAVSYIDL
                     NSEITSLTSILINLYNDKNDKIKLREKFAGEFMLHSKNEGYLKMLSQCEKVAKSNANV
                     LLTGESGTGKEVAAKYIHICSSRCTNNFVAVNCSSYTESLLESELFGYEQGSFTGAMK
                     SKQGKFEIANNGTLFLDEVGEINMTTQVKLLRVLETKKVERLGSNMEKLIDFKLISAT
                     NRNLVDDVMKSALREDFFYRISSIVINVPPLRERKEDLDDLVTYFLNLSQKENNIEIH
                     HIDDEAVKFLKSYSYPGNIRELKSIIDRMVVLSNDGLVTKEGIPILYNIHKKYETNKR
                     SSTENKFNKIIPFKQYKSEAEAEYLQWVLEQTGGNVAEAARQLEISTRQLFNKIKEYT
                     LQK"
     gene            24154..24546
                     /locus_tag="PKK75_02340"
     CDS             24154..24546
                     /locus_tag="PKK75_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HNZ82467.1"
                     /translation="MGKTIVTNNSKVYNKYKDLYELVYLEDGNYTDVLYNTRDFIHKG
                     FKLLTHPMAGSLKPNQTPYKSVIVEMCGKTDYESVELIENSIDAALKFLSIKKTPDWN
                     EKILNDFKTVDLSIIENVVKNPMFNMHK"
     gene            24586..25872
                     /gene="grdG"
                     /locus_tag="PKK75_02345"
     CDS             24586..25872
                     /gene="grdG"
                     /locus_tag="PKK75_02345"
                     /EC_number="1.21.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     alpha"
                     /protein_id="HNZ82468.1"
                     /translation="MKLELGKININDVQFGDKTEVVKGTLYVNKDEIIKLVLQDERII
                     KADVELARPGESVRIAPVKDVIEPRVKVSGSGAMFPGTINKVKQVGSGRTHALVGATV
                     ITCGRIVGFQEGLIDMSGPAAKYCPFSETNNVVVVIEPAEGLETHDYEAAGRIAGLKV
                     AAYVGEAGRNVEPDEVVVYETKPILEQAKQYPDLPKIGYVHMLQSQGLLHDTYYYGVD
                     AKVMVPTFMYPTEIMDGAIVSGNCVAPCDKVTTFHHLNNPVIEDLYKRHGKDLNFMGV
                     VLTNENVFLADKERSSDMTAKLCQCFGFDGVLITEEGYGNPDTDLMMNCKKVTQNGVK
                     VVLITDEFPGRDGKSQSLADAVEEGDALVSCGNGNVIVYFPPMEKVIGTLDFVETMIG
                     GYEGSLRADGSIEAELQIVIASTIANGYNKLAARGY"
     gene            25883..27193
                     /gene="grdF"
                     /locus_tag="PKK75_02350"
     CDS             25883..27193
                     /gene="grdF"
                     /locus_tag="PKK75_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:26930..26932,aa:Sec)
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     beta"
                     /protein_id="HNZ82469.1"
                     /translation="MSKVKVIHYINQFFAQVGGEEMADYEPEIREGIVGPGLALQQGL
                     GEDYEVVATVVCGDNYFGSHLEEAKQRILDMIRPYNPQLFVAGPAFNAGRYGVACGTI
                     AKAVEDEFNIPVVTGMYVENPGVDMFRKDLYIISTANSAADMRKSMPKMAKLAKKLVN
                     KEEILSPAEEGYVERGIRVNYFNERRGSDRAVDMILQKIKGQPYVTEYPMPAFDRVAP
                     NPAVKDISKVKVAVVTSGGIVPQGNPDRIESSSATKYGIYDITGMEEMTKDKFMTIHG
                     GYDRAYVTENPNLVVPLDVLREMEKEGVIGELANYFVTTTGTGTSVGNAKGFGEKFSK
                     KLVEDGVGAVILTSTUGTCTRCGATMVKEIERAGIPVVHMCTVVPISLTIGANRIIPA
                     VGIPYPLGDPNLGEEKSKKLRRTLVERALRALQTEISEQTVFEE"
     gene            27271..28410
                     /locus_tag="PKK75_02355"
     CDS             27271..28410
                     /locus_tag="PKK75_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82470.1"
                     /translation="MDKGKGFVSIKRVIIMGGAFIGLLIGSGFTTGQEIMQYFVSFGN
                     MGYAAIIMMFVLFVYVGSSFVAVGYEQKFDEPKNIYQYYCGKYIGTFFDYFSVIFLFM
                     SFWVMVAGAGAAFNQQFGLPNWVGGVVMGTATLITLYFGFSRLVDIIGSIGPVVSVLA
                     ILLGIAGIMMHPEGVSTFSTQVPALVESGTVLQAGTNWFMACASYVGFCMMWLAVFMT
                     NMGSTANSRREGIYGAIFGSSLYSVAVLLLMLGLGANLQEVAGAQIPVLILAKKISPT
                     LAIIYTAIVVTEIYTTAGPLLWTPVKRFAPDEKSSRYRMLLLVLGVIGIFVGLRVSFA
                     KLINVIYVINGYVGFMMLFLMIITDVRVRILKNYTPAIVTELKTK"
     gene            28639..29472
                     /locus_tag="PKK75_02360"
     CDS             28639..29472
                     /locus_tag="PKK75_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNZ82471.1"
                     /translation="MKYKMIVLDLDGTLLNDEKTISDKNAYILEELHKQGIHIVIATG
                     RNYYMARILIDRIKTTEPVILANNGAVVRRYINDEFIESNYLDFSIFEKIYELGIKQN
                     LNPVIHVDEYHQGYDLIYECEDYEKAYLGYIKKDYGRARNTKFQKEAISNILSVCYFN
                     EYEKLLKFKKEIEKIPGEFNVILNQNLKSLSLLELLNINGCKWCALKKYIDGLGIDKS
                     QVVAIGDDNNDIELIANSGLGIAMKNATGGLKDAADLISRFSNNESGVYYELSEIFEF
                     K"
     gene            29551..29742
                     /locus_tag="PKK75_02365"
     CDS             29551..29742
                     /locus_tag="PKK75_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HNZ82472.1"
                     /translation="MRIITAIASILIIIGALNWGLYGISKIDIVESLFGGWKNKFGRI
                     VYILIGIAGLYSLLYVIFA"
     gene            complement(29803..30021)
                     /locus_tag="PKK75_02370"
     CDS             complement(29803..30021)
                     /locus_tag="PKK75_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HNZ82473.1"
                     /translation="MQIKDGIVPAVLGTVVTATGVALRNTNLPESYRNGIIGFGLANI
                     ALGSAELVSSNWQHSRMMGKMAKMAPFK"
     gene            30165..31088
                     /locus_tag="PKK75_02375"
     CDS             30165..31088
                     /locus_tag="PKK75_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967214.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNZ82474.1"
                     /translation="MKLYNKYSDYLKSKYGEKVYKLPVNLPCTCPNRDGTLGYGGCTF
                     CADAGTGFEMLDSSLSVKEQLIRNMAYIKKKYKAEKFIAYFQNYTNTYMETERFKNYI
                     NDALMDDIVEIAVSTRPDCISDEKLKFLYEIKEFQNVNITIELGLQTVNYHTLQAINR
                     GHSLAEFVDAVLRCKGYNLEICAHVILNLPGDTILDTAETAKILSVLKVDQVKIHSLY
                     IMENTIMGELYKNNEITIISKDEYVERVVVFLEHLHKDIAVQRLAGRAPKENSLFVNW
                     GMSWWKIKDEILNKMESENRYQGSNNKFNKL"
     gene            31179..31592
                     /locus_tag="PKK75_02380"
     CDS             31179..31592
                     /locus_tag="PKK75_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82475.1"
                     /translation="MVQLVCGHSGTGKTRRMIEMANKAIDKIHGSMVFLEANNRHIFD
                     LDYRIRYINTKEFGLTNDVEFQGFVCGVIATDYDIEQVYIDGLYKIAKSGIEKLESVI
                     ERLDYLSKTFDVNFIISVNHDIEDIPEGLRDRVID"
     gene            31727..32281
                     /locus_tag="PKK75_02385"
     CDS             31727..32281
                     /locus_tag="PKK75_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82476.1"
                     /translation="MNYFKAKNTIFDDFFEHRDMLIIQHMNGDINKKEYLELNYKYML
                     EKNIKPFQRIDSFEKGMYNYQYYNMMAKYHRMIAQEIKDKGKHISYYSKYVNEADYYY
                     NEKDKTSFRLLRFLQFENVEAYFIKMESSILEGKLYEIVLKDYEYAVLHSKSLWLLEV
                     LKKEKVFTEKRKRSVIDYYVNQRY"
     gene            complement(32393..32842)
                     /locus_tag="PKK75_02390"
     CDS             complement(32393..32842)
                     /locus_tag="PKK75_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82477.1"
                     /translation="MKILKKIINAIRDFIYNITDYGLIIVVVAVMTVILAWRFDLLFS
                     RGIDKEMIPDLPIVNEPAEEEPEPVEEEPAEEIPSGIIATITIPQGSYPSKIGEILLN
                     NNLIESKEEFLTRCVELNLDTRLQSGTFDIELGTPLDDVIKIIANAY"
BASE COUNT        11021 a         5387 c         6604 g         9766 t
ORIGIN      
        1 atacaggttg aaaggataaa aacaggaagg atcctcacgg tataaatata tatgaattaa
       61 aaatacgaga cagacatatt aattaatatg tctgtttaat attgtaagga gtgaatatgt
      121 gagtaaactc tggtttggct tgatttccct ggggctttta ttttctttta taaataaaag
      181 tccggaaaca gtaacaaatg tccttttttt tgaaacagaa aagtcagttg aacttatttt
      241 aaatttaatt cctgttatgg ccttttggac agggttaatg cggatagtag aaaaaacaaa
      301 cattttaaac aagctgtcaa catcattaaa gcccatggta agatacttgt tcaaggatgt
      361 ccagcatgac gtaaaagctg taaacgcaat attaatgacc ttggctgcaa acatgttagg
      421 tataggaaat tctgccactg cctttgggat taaggccatg caggaaatgc agaagtccaa
      481 tgcaaataaa aaaacagcca caaacgccat gtgcatgttt ttaataataa acgtttcatc
      541 aattcagctg atacctttaa acattatcaa attaagagct gcatcaggat cctcggctcc
      601 ttctgaaatt atggttccaa ccttgattgt aactgcattt tcaaccatga tagctcttat
      661 atttgccaag tattatgaaa ggaaagagct atgaatattt cagatttaat acttcccttg
      721 ttgattgccg caatattgtt ctatgcagtt tcaaagggtg ttgatgtata ttcagccttt
      781 gtcgaaggtg caatagacgg agcaaggtca gcacttaaaa tatttccata tattttagca
      841 attatatttg caataaattt atttattcaa tcgggagctg aagcttttat tgtgggaata
      901 cttgagcctg taaccttatt tgcgggcttc cccgcagaat tgttatccct gtcgatagtc
      961 aagcctttgt caggaggagg ttcactgggg gtatttcaga caattttgga taattacggg
     1021 gctgattctt atataggccg gtgtgcatcg gttattatgg ggtcatcgga aacaatcttc
     1081 tatacaacgg cagtttattt cggctccgtg ggtatttccg attacagata tacaataaag
     1141 acgggacttt tgtctcatgc agccacaatt tttgccgcat tggctgtgtg cagattaatg
     1201 ttttgagcgc tttggcctta ttgaactctc ataaaacaag tcctgccaaa atgcaaaaat
     1261 tcctgccagt aaaaacacat cgccaacact tatggtttta gggaaaggat agggaggatg
     1321 cagggtgaat atatcacaca gaaactttag tctggtttct tccgtaagaa gggaatgaac
     1381 caggtctttt cctgaaagca gaaaattttt ttttgcttca acatcagtaa gaagttcaga
     1441 tacatacaca ggcatcttaa aatcatttgc caaaatagct gcaaagttaa ataaagtacc
     1501 gaaaaaaaca agcatcatga aagacttttt tatattgagc aatgaaacat aaaggagtcc
     1561 aaaataaatg acccagttaa gtgataaaat cctcagcaga cttacatttg taaatattct
     1621 aaataaaaat tcagcagcaa ttttaatgaa tgctgctaat atcaaaactt tatagcctct
     1681 tattcttact ttgtaccggt taaagttaaa ttttcttttt ttgattttta gaaatattat
     1741 tgcagttaca attgacagga gggaaaaaaa tacaatttca aaatacatct tataccctct
     1801 cttcagctaa tttaaaaaat gcttttttaa gtgcaattat agagtcatcc acaatatccg
     1861 ggttaaactg ggtacctccg ttatctttaa tttcctgaag tgctgattca agacttaagg
     1921 ctcctctgta aggcctgtca gttgtcattg catcaaagga atcggcaacg gtaagtatgg
     1981 atgtttccaa aggtatgtct tcgtggctca agccgtcagg atagcccttg ccatcatttc
     2041 tttcatgatg atgccttata atatctgaaa tatttgttaa ataacttaaa tctgctatta
     2101 ttgttgctcc tatttccgga tgggatttaa ttttgtcaaa ctcttccttt tccaattttc
     2161 cgtttttatt gagaatactt ttgtctatac cgattttacc tatatcatga agaagggctg
     2221 cactttttat taaatcaatt ttaccttggg gaagtcctac ttgatttgca atagctacag
     2281 catatgcact tacacgcatg gagtggccgc ttgtgtaggg gtcgctggct tcaattgccc
     2341 ttacaagaac attcaatgtg tcaaaataat ttttccgcat gtccagataa agcttaaatg
     2401 tgtatctggc aaacataaga ggaatgaaga acagcagaat tcctgttgtt ccaaaattat
     2461 cgtaggaaaa tgcaactaca attcccagca aaccaaccag gctgatatta ataaatccta
     2521 aaagatagtt tttccaaatg taagaaattt tttcgtttaa gagaattgac attaaactcg
     2581 ccataaaaac tgaattaaga gttaaataaa caaacaggca tacaacagca gcaataggat
     2641 taaaaaaata aaatccgaaa tttaaaattc tattcaaata taaatatgtt aatcccgctg
     2701 caccagcatt gataatgtac tgacttacat taaaaacagt tttatatata ggattgttaa
     2761 acatatgaac tctgcccttc ccatctacat aggggcatct gaatatcatt ccgaaggctg
     2821 caatcaagga cgctgttaat gggtttgtaa gcagtattgc agaaaaggtg agtgcaaaac
     2881 tcacagatac agcacctacc ttaggcaatg gaatcagaaa tgtttctgct aaagcagata
     2941 aaactgaaaa aacaataagc aaggtataat cgcttattgt atagttttta aataaataaa
     3001 tcaaaagcat taatgaagtt aatgcaagaa aaataatata tgcataaagt ttaatattcc
     3061 gttttttact ttgcttaata ttattcattt tttatccttt aatatgaagg gctgacttca
     3121 tttactattt tttgattata accaactata gcctgcgcct gctgctaata ccattgctac
     3181 taatgtagct attataggaa ataacttttt catttgaaac ctccgctgag aatctctgct
     3241 cagttcgcta atagaacagg aaggttccgc tacgctattt gatatttaag tcagccctat
     3301 cattctttta tcaataaaca gatttttctg ttaattgcat ttaatgttgc ctttattaca
     3361 gaatcaattt tattgttatc aacaacggaa gcccctacca gaatgttttc cttgccgtta
     3421 ttaatttgat taatagctac aacaagtgct tcgcctccga tgattcttac gttttgaata
     3481 tcttcaacaa tgaaacaatc aaaaccgatg gcttgcttga ttgcgtctaa agctgatctt
     3541 gcagccacta tcaaggattg tttctctgat tttattcctt cagaaactcc taaaaattcc
     3601 ttgttgtccc aggacagttt tgttttaatt gagattctgt cgcaagttgt ttcgattgta
     3661 tttccttcat acaggagtct gaagtctgaa ttgaaggata tatccttgtc aatttgcgct
     3721 acgctgatta ttttataatc aacgtctgtg ttaaacttag aaagcagagc agttcgtata
     3781 tctctcgcta tttgtttgga ttgcctgctg ctgtcggata atacatgtat ttcgcttatt
     3841 tcgttgttag aattcattat aaccttagat gatattacag acttgatgct atttaaaaaa
     3901 tactccattt ctccgaaatt catatttatc ctccaagtat tattaaattg attataatac
     3961 aataatatta aaatttcaac atttaattag caaattatat aaaattttct cattttcttc
     4021 ataattctta caaaaaaaat acactattcc atatttgacg ggaaagtgta ttaattactg
     4081 taaatgttta attagccaac atgacgaacg gaaatttctt tgcgcctgct gaaccggcac
     4141 aagaaaaata catatatata tttatagttc attgaagtat gtaattatac ctgtataaac
     4201 agcccacgct gttttttcct gataatcgcc ggaaatcaat tttctttctt cattattgtt
     4261 tgataggaat ccgcattcta ttaaaattac cggaagactt gtgtcatcca ttatttttat
     4321 gccctttttt atttgtggta cacggctgtt atttttgtcc agaatattct tcatgctttc
     4381 ctgtattgca tcggcagcta attttgttga aatttcattg cttttttgat aaaaaacatg
     4441 agctccgctg tattgtttct gaggaaagga attcaaatgt attgatatta ccaaatccgc
     4501 attggaactg tttatcagtt caactctgtt ttttaaatct tcattttttt tggctctgat
     4561 agtgccggaa agctctgtat acaagccgct gtcatcatat cttgtcattt caacttcaaa
     4621 tccgctgcct tccaaaaaac tcataagctt tttggatatg gctaaattta taggggcttc
     4681 tgctactttc atatcaccgc tggctccctg gtctactcct ccatgtccag ggtctattag
     4741 aattttaatt ttttctatct tttcttcgcc tgcagcatca atagtttctg cagtccttgt
     4801 taggtttaag aaaatactca ccagcattat aattattatt gcagcagcaa ataccatatt
     4861 aatctttttt aaaatatttt ccataatatt acctctcata atttatctca tatttaaatt
     4921 tatgaagggt aataattaat attacaaaaa agagagacac gtcttataat gtcaccctat
     4981 gcttattcaa attcttttga caaaggtatc ataagcaggg acacagctta tgccggctgc
     5041 ccctgtagga taaaaaacag gaaggcaagc agtgtcccgg ttagtttctt ttccaaatta
     5101 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagaatatc ataatgcttt
     5221 ttaaagtgtc atcatctaaa gacttgccgt caactttaat agtttgtaca gacttaggat
     5281 gaattgtgtg cttgatttca taataaaaag ctttaaaaag cactaaaatt ctaacggatt
     5341 taacaccgcc ggctgttgat ccggcacagg caccaaaaaa cattaaggct aagagcaccg
     5401 attttgaaaa cattggccac aaattgaaat ccgtcgttgc atagcctgta gttgtaacca
     5461 gggtggaaac cgtgaaggca gaatatctta acgcttcaaa aaagtcatag ttgaacctgg
     5521 gaattacatt aattacaata agcgctattg ctaaacctgt gtaaaagcag tagagcctga
     5581 attcttcgtt cttgaaaatc tgtttgaatt cttttttgat aagaagatag taaagagtga
     5641 aatttacccc gaataaaaac atacctattg tgattatcca gtcaatccaa gggttattat
     5701 aggctcccac acttaaagca tccatggaga agcctccggt tcctgcagtt ccaaaagcat
     5761 aaatgaatga attgaacaca ggcattccgg cagctattaa gagtataata acaattacag
     5821 tcataactgc ataaattata tataaaattt tagatgattc tcctatttta ggaaccaatt
     5881 ttcccggtga gggacctggg ctttcagcac gcattaaaaa taccggacgt ccaccgattg
     5941 atggaaacaa agccatagta aataccaata cacccatacc gcctatccag tgggtaaaac
     6001 ttctccaaaa caaaagtcct cttggaatga tttcaacatc ccttataatg cttgaacctg
     6061 ttgtagtaaa tccggaaaca gtttcaaaca gggcatcaat ataggatggt atggagccgc
     6121 ttaaataaaa aggcagagca ccaaaaatgg acattacaag ccagctgagt gcaactgctg
     6181 cataaccctc tctttttcgc atttctgcac tgtcgatttc aaatcttgaa aggataaatc
     6241 ctgttacagc cgtggctgca atggttataa caaaagcctt gatttcataa gagttgtcaa
     6301 ttattgatat caacaatgaa ggaaccatta atacagcttc ccaaaataat actattccaa
     6361 gtactttaag tactgacttt actttcacaa aatgcctccg gttcattgag attaataaat
     6421 gttatgccaa tatttcctta aggtcggcta taaacccggt cttggtaacc actataacct
     6481 tgtcgtgctc tttaattaca tcatttccat taggaatgat aattttttta ttacgtaaaa
     6541 ttgcagctat taacacatca tttttcaggt ttaaatcctg caatttaaca tctgttaaat
     6601 cagaagaatt ttttacagta aattcagcga cttcagcctt attatcaagt actctgtaca
     6661 gtttttcaac agctgttccc tttgtattat tgagtgctct tatatatctc aatatattgg
     6721 tggcagtaat aaactttggg cttattatgc tgtcaagtcc tagttttttc actattggaa
     6781 aataattttg tctgtttatt tttaaaatta ccttaggcac accgcattgg tgagcataca
     6841 aggaacttat taaattttct tcgtccctgt cggttaatgc aacaataaca tcagctgctt
     6901 caatattttc attatccaat acttcctggt cagtaccatc cccgtggatt atcaaagcat
     6961 ctgtaagaat gtcatttaat actaagcacc tatccttgtt tagctcaatt atttttacat
     7021 ttaaacccat ttcctccaga atccatgtta agtatatagc tgtcctgctt cctccaataa
     7081 tagttacatt tttagccttg tgtgagcttc tacccaagta cttgaaaaat ctcgagatgc
     7141 ttattctttc gccgattacc tgtattatat catttggtaa gaatgaaaag ctgccgttag
     7201 gtataaaaac ttcttcatct ctcattaccg tacaaaacaa tactcttgaa ggcaaatgcc
     7261 tgttaagatc cataagcttt tgatttatta tgggatctgt tcccaaaact ctgaactcaa
     7321 ccagttcaat ctggttattg gcaaatgttt caacatctgc agcagagggg aactgaacca
     7381 tttgagcaat ttcagctgct gcttccattt ccggattaat aaccatatct aattcaagtt
     7441 cttcccttaa cattttaaat tcatcagaat aatcagtgtt ccttacccgg gcaacagtat
     7501 gctttgctcc taatttcttc ccgataacgc agcaaatcat atttagttca tcgctgtcag
     7561 tcacagctag tataatatca gtttcaccta cacctgcact tcttaatatc tctgcacttg
     7621 caccattgcc tttgatacac ataatatcaa gagtttcatc tgcatgagtc agcacgttgg
     7681 catcattgtc caccaaagtt atatcgtgat cttcagttga taattgctga gccaatgtgt
     7741 agccgacttt tcctccgccc acaattataa ttttcatttt ctcctccaaa atttaataag
     7801 tttatcaata tataaattaa aaaagacaac caaattattg gctatccacc ctgatagttt
     7861 actctcacat aaggacatca tatccaaaag gacttagatg gcttactccg caaataccgt
     7921 tttcactaat atcgctatga ccgcaagaaa aattttattt tctttaaaac attatatggt
     7981 tttatttata ggatgtcaaa taattttgta ttaaattata gtaaaaatat aagagccgtt
     8041 aatattatac caacattcca gagagataca acattatatt tgcaatcaac ataatatcgt
     8101 aaaattgaca cagttaaatt tttattatat aataaaaacg cttaaattta ataattattc
     8161 ggatgcatag tatttcgaat acccaaacat acttgacagg aggcatcatg agaattaagg
     8221 aacacccgat actaaagttc aataaaaaca agaaaataaa gtttgttttt aacggtcagg
     8281 aaatggaagg ctatgaggga gaaacaatcg ctgccgcctt gcatgctgcc ggtgtgaagg
     8341 ttctcgggaa aagtttattt ttgcacagac cgagaggctt ttactgtgca atagggaatt
     8401 gctcttcctg cttaatgaca gtaaacgggg ttgcaaacgt aaaaacctgt gttactgatc
     8461 tggaagaagg aatgatggtt gaaacccagg aaggcaaggg agtgattgta tgaaacatgc
     8521 agaaattgtt attgcaggcg gtggtcccgc aggtctttgt gccggcataa gcgcagccaa
     8581 aagcggcgcc agggttatga ttattgataa aaataaaaat ttaggaggcc agcttgtaaa
     8641 acaaacacat atgttttttg gctcagaaaa acaatatgca tctacaaggg gaattgacat
     8701 ccccgaaata ttgataaagg agcttgacaa atataaagaa aaagtcgaaa taaaaactaa
     8761 ctcaacagta acagggatgt atgaagatgg aatgataaca gttttagaga aggaagttaa
     8821 ttattataaa ataaaaccta aagctgtaat tgttgctaca ggagcctatg aaaaacactt
     8881 aagctttccc aataatgatt tgccgggaat ttacggagcc ggagctgttc agaccctgat
     8941 gaatgtttac ggtgtaaagc ctggagacag ggtattgatg gcaggagccg gcaatatagg
     9001 gctaattgta agctaccagc tgatgcaggc aggagttgaa gtaaaagcaa tacttgatgc
     9061 agccccaaaa ataggaggct acctggtaca tgcctccaaa attagaagaa tgggtgtacc
     9121 aatacttact tctcataccg taaaagaagc aataggaaaa gaatttttgg aaaaagcagt
     9181 ggtgtgcaag gttgatgaaa aattcaaccc tatagaaggt actgaaataa cctttgatgt
     9241 tgatgtaatc tgcatatctg taggtcttgc accgctgaat gagcttcttg caatgagggg
     9301 ctgtgaaatg aagtatatac ctcaattaag cggccttgtt cccgtgagaa atgaaaacta
     9361 tgaaacaaca gttgaaaata tatttactgc aggagatgct tcaggagttg aagaagcaag
     9421 tgctgccatg gttgaagggt ttttggcagg actatgcgct gcagcaaaaa ttggatataa
     9481 acctgatgat tttacagaca gaaaaaatga ttatttgaaa caacttgaaa atttgcgctc
     9541 aggacctgcc gggaagcata ttttagcagg cattaaagag ataggaggca aataatgttt
     9601 gaaaaaaccg gaataccaag catggaaatg ataatggaaa agtttccttc aatagaaaga
     9661 atcaataagg gtgcagttgc agtaattgag tgctacaaag aaatcccctg caatccctgc
     9721 gagaccgcct gcaaatttga cgcaataaaa gtaggtgaag atataaacaa catacccagg
     9781 ctaaactctg gaaactgcac gggctgtgga atttgcctga gcaaatgccc ggggttggca
     9841 atcatgatag tggacggttc taaatcagaa actacagtcg aagttaaaat accatatgag
     9901 ttcttgcctc ttccaaagga aggtcaaata gtaaaaggtc ttgacaggga agggaaatat
     9961 ataacagatg taaaggtatt aaaagtatta aaccccaaat cctttgacag aacaccggtt
    10021 attacgattg aagcggacag gaagtttttg tatgaaatca gaaatatcat gttggaggtc
    10081 taacatggat gaaaatacta taatctgcag atgctcagat gttacattaa aggaaataag
    10141 agagctgata aatgatggct atgttacatt tgatgaaata aagagaataa cccgtgcagg
    10201 catgggacca tgccagggta agacctgcgg ctatctgata atgagagaaa tcgcaaatgc
    10261 tacaggtcaa aatttaaagg atattaaatt tcaaaccaac aggcctcctg tggtcggagt
    10321 aaaactgggt cttatcgcca aggagggaaa aaatgaaaag taaagcggaa gttgtaataa
    10381 tcggaggcgg aatatccggc tgtgccattg catataactt agcccaaaaa ggagttaagg
    10441 atgttgtagt actggaaaag gaatatattt gcagcgggtc cacgggcagg tgcggtgccg
    10501 gtgttagaat gcagtgggga acagaaatga actgcagaat tgccaagaaa agcatcgaat
    10561 tttacgaaca tgcaaatgaa attctggagt atgaaagaga tgtggaattt aaacaaaaag
    10621 gctatctttt agttgcagat acagaccatg aagtggacca gttcaggaag aatattgagg
    10681 ttcagcattc atgctgcatc ccatccacaa tgctttcatt ggaagaagca agggaaatag
    10741 tcccttacct gaatacggat atattgaagg gagcggccta ttgcagcaag gatggctttt
    10801 taaatccatt tcatacgacc gatgcttttt acagggctgc aaaaagactg ggagcagaat
    10861 ttaatacttt tactgaagca ataggaatca aggttgaaaa aggagaggta aaaggtgttc
    10921 aaaccaacaa aggttttatt tcaacaaaca ttgttgtgaa tgccgccaac ggctacggca
    10981 aaggcatctg cgacatggtg ggtcttgata tacccacata ttcagaaagg caccagattc
    11041 ttgtgacaga gcctgttgaa cctatgcagg accccatggt tatgtctttc ggaatgaatt
    11101 tttacatcca gcagtcaccg gaaggcactt ttataatggg caggggcgat gaaaatgagc
    11161 ccagggattt aagagttaca tccagctggc agtttattga ggaaatggcg aagacaatag
    11221 acctggtcct gcccccaata tcaaaattga gggtaatcag acaatgggca ggtctttaca
    11281 atatgacgcc tgacaagcag ccgatttatg atgaagcgga aaatgtaaaa ggattttaca
    11341 ttgctttggg atacagcgga catggtttta tgtttgggcc tattacaggt gttgcaatat
    11401 ctgaaatgat attgaaggaa aaacctacaa tagatgtaag catgcttaat ttaaaccgct
    11461 ttaaaacagg caagcttctt ttggagccat cggtggttta aaggtatggg gacacacctc
    11521 catgcggggt aagcctctgg agtgagacca tgaaggaatg tccccaaatc ggttgctttt
    11581 tgtgttagat agagtataat gcaaatagga atgtcattct gaaggcagtc cttatgcgca
    11641 gatgacaaaa aaacagttaa ttttacggga ggctgaaatg agagaaaaat atttaaacaa
    11701 gctggttgac atattgaaga aggaaaaaat tgatgccata ttaattgctc cctctgaaga
    11761 aatggaattc ataatgggac acaatactca tctttgcgaa aggtttcaag cattgattat
    11821 taaaaatgac ggcagctact tttatatttg caatcttctg acacaggatg aaatccaaag
    11881 tgtgttaggt ccggatatca aggtttacgg ctggtttgac ggagataaat ttactgacac
    11941 ggttaataag gcattccagg agcagggact gataggaaag actgttgccg tgaattctac
    12001 tgaaagagca ttcataattc ttcagataat ggaagcaatg gatgttaaat ttataaacgg
    12061 caagcctttt ttggaagaca tgaggattat aaaggatgaa gaagagcttg aaaacctaag
    12121 aatggctgcc cgtattacgg atgagtccta tgatgagctg cttaagttta taaggccggg
    12181 aattaaggaa gctgatattg cagaaaagat gaaggaaata ttcaaggaaa aaggtgccga
    12241 tgaaggtttt accatggttt gctcaggacc taattcatct tacccccact acaatgatga
    12301 tcaaagggta attcaggaaa aggacatcat aatacttgac tggggatgca aatacaaaaa
    12361 tatgtgcgcc gatatgtcaa gaaccatatt tgtaggcggg ataacagagg aagaaagaaa
    12421 ggtttatgaa atagtgagag ccagccagga agccggagaa aaagcagccg tgaaaggagc
    12481 atacataccg gatgtggata aggcttcaag agatataatc caaaagtcag gatacggcag
    12541 atactttttt acaagactag ggcacggaat aggatattca gtacatgagg caccggacat
    12601 taaggcaggc aatgaaagga acctggaaaa aggcatggtg ttcagcattg aacccggcat
    12661 ttatattgca ggaaaatttg gaatgcgcat cgaaaatatt gttgctgtta ccgaagatgg
    12721 aaatgaagtg ttaaataagg caacaaaaga aataatgata gtttaaatga agggggacac
    12781 gcctccatgg cagggaaact gttggagccg gaccatgaag gaatgtcccc gtacttgtta
    12841 attataaagg agagcaaatg gatttcaaag aacaagcttt agagcttcat aaaaataatg
    12901 ttgtgataga cgctcactta gatctggcag gggaaattta caacagatac ctggcagggg
    12961 aaaaggatgt aataaaaaat cattacctgg acagctttaa aaagggcggg tttaacttaa
    13021 ttgtatcttc tttgtatata gacgaaatat ttctgcctga aatggctctc aggactgctt
    13081 taggacaaat gagggcattg attgaggatg tggaatcttt agatggtgaa gtaatattgg
    13141 taaaaagccg ggacgacttg aataaggcgg taaaagagaa caaaatcgga atagttattt
    13201 ccttcgaagg cttggagcca ataggcaatg acattggtct tttaaaaatt ttttacgaac
    13261 ttggcgtaag ggctgcagga ctggtatgga gcaggagaaa ttatgtgggt gacggatgca
    13321 gctttagccc tgttgaggaa ggtcaaagag ggggccttac aagattcggc gttaatgttg
    13381 taagaaaaat ggaagagatg aatatgctta ttgatgtgag ccatttaaat gatgaaggct
    13441 tttgggacgt ggtaaagttt acaaacaaac cttttattgc ttctcattcc aattccagag
    13501 ctgctcacgg aatgatgaga aatctccagg atgatcagat aaaagctata agggacagaa
    13561 atggcgtcat aggaataaat gcatatacaa atattttagg agtaagagaa ggggagaatt
    13621 ccataagcag gctggcagat catattgaat atatggtaaa tcttgcagga cccaggcatg
    13681 ttggctatgg ctttgaccta tgtaacggct tttacaacag cgcattaaaa tataaatttg
    13741 agccccgtaa cagtgacggt ttaagttctc attcggaggc ggtaaaactg acagaagagc
    13801 ttttaagaag aggaatttca caggaagact gcaagcttat aattggcggt aatttcctaa
    13861 gagtttatag tgatatttta atctaggggg aaaacaatta tgactgattc aaacaggcaa
    13921 agaatttggt attttattca ggaatgggtt tttcctgtta taattgcctt aattattgtt
    13981 ttactgctta ataaatttgt attcatcctg gtaacagtac ctacagattc aatggaagat
    14041 accataatag ccggtgacag gctgtatgta aatgaactgt ttgatataaa taatgcaaaa
    14101 agaggggaca tactggtatt caagtctgat gaactggagg ataaaagact ggtgaagagg
    14161 ctgataggat taccgggaga aactgttgaa ataaaaaggg acggaagtat ttttgtcgac
    14221 ggaaaaaaga ttgaagagcc ctatgcatta gagtcccatg gagaagaaaa agtatttaca
    14281 gtacccttga attcatactt ttttttgggg gacaacaggc ctatatccta tgatgccagg
    14341 tactggaatg aaccttacat aggtgaagat aaagtaatag gcaaggttga attcagattt
    14401 ttccctttta acagaatcgg tgaagttgaa taactgactg caggagggac actactaaag
    14461 ggagaagcca ataatggtat ggggacacac cattatttat cttgatagtc tttgagtgta
    14521 aattgaatgt ccccgattaa gataaaggaa ggtctcacag gaggattata tgaagagagt
    14581 tttagaagga ttaaaaccgg aaaaggtgtt ttattatttt gaggaaatat caaaaatacc
    14641 ccgttgttca ggacatgaaa agcagatttc agattatcta tacaattttg ccaggtcaaa
    14701 aaacctggaa gcaatccagg atgagtattt aaatgtaatt attaaaaagc ccgcttcaag
    14761 gggatatgaa aatgcacctg cagtaatgct tcagggacat atggacatgg tttgtgaaaa
    14821 aaatgagggg gttgaccacg atttttccaa ggaccctata aaattaagaa ttaaggatgg
    14881 ctgcatttat gcatcagaca caaccttagg ggctgacaac ggtattgcag tggcaatggc
    14941 attgtctgtt cttgattcgg atctagaaca tcctgctctt gaggttttaa taacaacaga
    15001 tgaagaaaag ggaatgacag gagcaaataa acttgacggc agcctgttta aaagcaagta
    15061 tctgctaaac atggacagtg aagaggaagg agtgttcact gcaggatgtg caggaggcag
    15121 cgaggttgat tttaaaatac ctgtaaaatt tcaaaatata ggaggcaggg catacaaaat
    15181 aagtgtcaaa gggctgtcag gtggtcactc aggcgtagac atccacaaag aaaaaggaaa
    15241 tgcaaacaaa attctaggca gaattcttta tgatttaatt gatgatacag aactattcag
    15301 tattgaggga ggctcaaaaa caaatgccat accaagagaa gcaaatgcgg ttctaacaat
    15361 taaagattta gataaagtat cggaaaaact cgataaatgg aatgaaatac ttaaaaatga
    15421 atatgcattt acagacccga atgtaaaagt gacattaact gatttaaaag aggaagtatt
    15481 tccgctggat aaggaaatat acaaaaaagc tgctactctg ataaacttga ttcctgttgg
    15541 tgtattatct agaagcactg ctattgattt ggttataagc tccaataact taggggttat
    15601 aactacagat gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgctatt
    15721 gatttggtta taagctccaa taacttaggg gttataacta cagatgaaaa atatataaga
    15781 ctgttcaatc atcccagaag cagtgtggaa acattgctta ccgaaggttt tattcctgca
    15841 atggagcagc tgggggacat gcttggtgta gagtgtgaaa ggggaagtta ttatcccggc
    15901 tgggaatatg caaaagaatc tgaaatcagg gatatttgca ttggagtgta cagggatatg
    15961 tttaaaaagg agcctcttgt aggtgcaatt cacgcgggac tggaatgcgg actcttaatg
    16021 gataaaattc cggggcttga tgcaatatca atgggacctg atgcatatga cgctcattca
    16081 cccaatgaac atgtaagcat aagctcagtg gaaaatatat ataatttatt gtgtgaaata
    16141 ttaaaaagaa taaaatagct taccgtggct ttgcccatat atgttatact tgttttcatt
    16201 ttgttaacat acaaacctga atgaatgcgg attaaagcta acagccaaaa aaagattttt
    16261 cttaaaaact gtatgcatat tcatagcaaa ttaaaaaatt tctgtaaact atctttatat
    16321 attaatcgtt taaatggatt taacaacgct ttcctgctat aattatacag cataggcata
    16381 aaaataaaag tgttgatata ataaaactaa catgttataa taatctttgc ttcaaataca
    16441 ataaggatga ttccaaacat ccttatcata ttcctgaatt taatttattg aaatattgga
    16501 ggtcgtgatg gattctaaat tagcaatata cattgttaag agaatggctt tggcagtagt
    16561 tacgatattt ttgattataa ccataacttt ctttgcaatg cataacatac cgggtggacc
    16621 gtttttaagt gaaaaggcag caacccctgc agtaacgaaa gcattagaag caaaatacgg
    16681 actggacaaa cctttatcag aacagtacac aacatattta aagggagttg caaagtttga
    16741 ctttggtcct tcccttaagc aaagaggaag agaagtaagc accgtaataa tgacaggttt
    16801 taaggtaagt tcgaaactgg gaggaacagc tgctttaatt ggactttctc tgggaatgtt
    16861 ctttggttct ttagcggcgg tttttcacaa taaggctata gataaaataa taatggttat
    16921 atctacagct atggtggcca tgccctcatt tattatagca acattgcttt tgctgatctt
    16981 ttgcgtctgg acggggatat ttcctgccaa cggaaatgag cctggaggac ttatacttcc
    17041 tataatatca ttaagtttat atcctatgtc ttatactgta agactgacaa gatcaagcat
    17101 gcttgatgta ttaagccagg attatataag gactgcgagg gcaaaaggag ttaagccatc
    17161 aaaggtttta ttcaaacatg ctctccgaaa tgcagttaca cctgttataa catatgccgg
    17221 tcctttgatt gcttatatta ttacagggag tttagttgta gaaaaaatat ttgccgtgcc
    17281 gggacttggg aaagaattcg tttcaagcat atttaatcga gattatacat tgattatggg
    17341 aacaactata tttttgggag cgcttataat tgttatgatg cttgttacag atataatgta
    17401 caaggttttt gacccaagaa ttcaattagg atagaaagtg gggataaaga tgaataatat
    17461 aaatgatttg cctaaaaaga atcctcttag ctttcagctt aatttgaagg attttgaaaa
    17521 agcaacagat gaagaaaagg ctcaacaggt aagaatgagc gaaagcgtaa cattctttaa
    17581 agatggtata agacgattaa aaaggaatag aattgcaatg gcttgtttgt ggattatagt
    17641 tgctattgca atattggtaa ctattgtacc tatggtttat ccatatacct atgagcagca
    17701 attaggggtg gctcaaggaa agagaacaga tagttcatat aataatttaa agccctttga
    17761 atatggtgaa acagaattaa aacgtataga gagcggggaa aaaatatttc ctcacatatt
    17821 tgggactgat gcgtcaggga gagattatgc cataagagta atatttggag caagaatttc
    17881 attgcttgta ggattcttcg cagctataat tgtactgatt ataggtgttg tatacggttc
    17941 tatttcagga tattttggcg gaaagacgga cctgatcatg atgaggatcg tggatatcat
    18001 ttattcgctt cctgatatgc ttatgattat tttactttca gtagtattaa aagaagtatt
    18061 aacacctaaa atagaaggaa catcccttgc caaaataggg acagacatgc tgagcatatt
    18121 tattgtattt gcattgttat attgggtgag tatggcaaga cttgtaagag gccaattact
    18181 ttcaataaag cagaacgaat atgttttagc agcaagatcc ataggcgcca gcccctggag
    18241 gataattaag aagcatttgc ttcctaacag catgagtgtt ataataatat ccactgcatt
    18301 acagattccg tcagccatat ttacggagag tttcttaagt tttcttggcc tgggtgtttc
    18361 cataccaatg ccaagtttag gttcattggc atcggatgct ttgtcgggga tttacagcta
    18421 tccctacaga ctaatcatac ccgcactttt tatatggata atagttttat ctttcaacct
    18481 gttaggtgac ggattaaggg atgcatttga ccctaaatta aagaagtagg aggaataaaa
    18541 tgagtttatt atcagtaaaa gatttgcata cttcattttt tacatcttcc ggtgaggtaa
    18601 aggcagttaa tggtgtatcc tttgatttag atgaaggtaa ggttttggga attgttggcg
    18661 aatcaggctc aggaaaaagc gtaacagcat attccatcct gcaaatcctt tcaaatcctg
    18721 gaagaatagt aagcggaagc ataaaattaa aaggtaagga attggttggc tgcagtaagg
    18781 aagaaatgca aaaaatcaga ggaaatcaaa taagcataat tttccaggac ccaatgacaa
    18841 gtttgaatcc tgttttcacc ataggaagtc aattaatgga agcaatttta ttgcatacaa
    18901 aaagaaacag ggaacaagct aaagaaagag ctatagaaat gctaaaactg gttggtgtaa
    18961 atgaaccgga aagaagaata aaccagtacc ctcatgaatt ttcaggcggc atgagacaaa
    19021 gagttatgat tgccatggca cttgcatgtg aaccggatat actaatagcc gatgagccta
    19081 caacagcctt ggatgttaca atacaggcgc aaattcttga tttaatgaaa gacttgcaga
    19141 aacaattagg aatggctata attcttataa cccatgattt aggggtcgtt gcagaaatgt
    19201 gcgatgagat tatcgttatg tatgcaggtg aaatatctga aagaggcaca tccgatgata
    19261 ttttctacaa tccaaaacac gagtatacaa aaggactttt aaaatccatt ccaacttcac
    19321 aaaatgaaaa aatgcgttta aaaccaattg gagggaatcc tgttgattta ttaaatcttc
    19381 ctgacggatg cgccttttca ccgcgatgtg aaaatgctat gaaagtctgc ctttatcata
    19441 atcctaaaga attaaaagtt ggagaaggtc atgcttcaag gtgctggatg aatattaaat
    19501 caggaattga ggaaaacaag cttacaaaag aagaagtaga ggagatgtat aaacatgaac
    19561 agcaacaata acaatcttct tgaagtcaag aatttaaaac aatactttga tatttccaca
    19621 ggcttgttaa ggagtaaacc tttaaaagcc gttgatgata tatctttcag cataaaaaaa
    19681 ggtgaaacat taggcctggt aggagaatca ggctgcggaa aaacaacagt cggaaggtca
    19741 atattacagt tatataaacc taccggcggt gaagtgattt ttaaaggtaa aaaaatagga
    19801 agcaaggagt caataaggaa tttaagacaa aatgcccaga tggtttttca agacccgtat
    19861 tcatctttaa atccaaggat gactgtcgga gatatagttg gagagccctt agacgtattt
    19921 aaactttaca aaaacagcaa ggaaagaaat aataaaataa atgagttaat ggatatcgta
    19981 gggttatcaa gtgagcatgc tacccgttat gcacatgagt tttccggagg ccagcgccaa
    20041 agaataggca ttgcaagagc tcttgcagta caacctgaat ttattgtgtg tgatgagccg
    20101 gtatcagctc ttgacgtatc aattcaagca cagattataa ataagtttga agatttacag
    20161 gatcagctgg gtttgacata tttgtttatt gcccatgatt tattggtagt aaagcatatt
    20221 tcagatagaa ttgctgttat gtatttggga agaatgatgg aacttgcaga ctcagatgaa
    20281 ttatataatc atcctttaca tccttacact atttcattgt tgtcagcggt acctgtacct
    20341 gatcccaatt atgcaagaaa tcacaggagg attttgcttg aaggggatgt tccaagtcca
    20401 ttaaatatgc ccagcggatg tcctttcaga acaagatgcc aaagggcaac aaaggaatgt
    20461 gccgaaacca ggccggaatt aagggaagta tcacccggac atatggttgc ctgtattcat
    20521 gtttaaaggt atggggacac accttcctga ggcaagtcaa agaggcaagt cagaaggaat
    20581 gtccccagat taagagggac acacagatgt cccctgctta aataatttga tattaatttt
    20641 taaattaata tatatatatt tattaggagg aaaatatgaa aaaaatctta tctttgattt
    20701 tagttttggc tatggtgttt tcattagcag catgcggaaa taatcaacca ccggcacagc
    20761 ctgaaacacc agagacaccg gaaacaccgg aaacaccgga aacacctgaa gctcctggaa
    20821 ttgcagaaag cataacagtt agcgtaggtg cttatccgga cactattgac ccggcactaa
    20881 acagtgcagt tgacggggca acttatataa tccatacatt tgcaggatta gttggttaca
    20941 gacaaaaagc tgatggcagt ttggaactgt atgctgattt agctaaagaa ttacctgaag
    21001 ctcaacctac agatgatgga aaagttaagt atgtttatga attgaaagat ggtttgaaat
    21061 ggaatgacgg ttctgattta acagctcaag atttcgttta tgcatggaac agagctgcag
    21121 atcctataac aggagcagac tataactaca tgtttgaagt tgttgacgga tacgcagaag
    21181 tgtctgagtc tgatgatgaa ggcaacagag taaatcctga tgcaaaactt aatgttacag
    21241 cttccgaaga cggaaaaaca cttacagttg ttcttcctat agatgttcct tattttaatg
    21301 aattagcagc attcccaaca tacatgcctg taaaacaaag tgttgttgaa ggtaatgaag
    21361 catgggctac tgcagctgaa acatatatag gaaacggtcc ttacaaagtt gtggaatttt
    21421 cacaaagtga attgataatg aaaaagaatg agtactatca tgataaagat tctataaaaa
    21481 cagaagagct ggtatttgct tttaatgacg atgatacatc tatactggca aacttccaga
    21541 acggttctta catgtttata gactctgttc ccaatgatga aatcaaatca ttgcaagcat
    21601 caaatcctga tgaattcttc attgaaggac aacttggaac atactatatt tcacacaatg
    21661 taaatgacac agcattcgct aagttcggcg aagctgataa agcaaaaata agaaaagctc
    21721 ttggcttaat gatagacaga acatacattg ttgaagaaat aggtcaggca ggtcaggttc
    21781 ctgcagcagg tttcgttgca atgggtcttt tggagccgga cggagcaaca gaatacattg
    21841 cagtaaacgg tcctaacaga gacggaaaag gctactacag tgttgaccct aatgattatg
    21901 aaaataattc aaaagaagca attgcattgc tgaaagatgc tgcagaatca tccaaactgt
    21961 tcacagttga cgatgcaggc aaagtatcga gcgaattccc aacagttgct tatattacaa
    22021 atgttggaac aggtcatgaa agaatagctg aatatcttca agctactttt gcatcatatg
    22081 gaatcaatat gacaattgaa aaacaagagt ggggaacatt ccttcaaact cgtaaacaag
    22141 gaaattattc agttgcaaga aacggctggt taggtgacta caatgatcct atatcattcc
    22201 ttgacatgtg gattactaca agcggtaaca acgattcaca gtttggcaag ggtgaacatg
    22261 ctaattatgc aggatacact tacaaaggtg aaggcggaaa aacatgggca gagtcttatg
    22321 ataagataat ttctgaagta aaagcttcca aggatgcagt agaaagattt aaattaatgc
    22381 atgatgctga agacatattg atgtcaacag gagctataac acctatttat tactacacag
    22441 atattttcat gatgagcaaa gatcttgacg gtgcttttgc aagcccgcta ggatacaaat
    22501 acttcatgta ttcatcagta gctgaataag atcacagaaa aattaaagcc gggagaaatc
    22561 ccggcttttt tacttttgca gagtatattc ttttattttg ttaaaaagct gtcttgtgct
    22621 tatctccagc tgtcttgccg cttctgccac gttgccgccg gtttgttcca ggacccattg
    22681 cagatactcg gcttcagctt cagatttata ctgtttaaaa ggaattatct tgttaaattt
    22741 attttcggtt gaacttcttt tattcgtctc gtatttcttg tgtatgttat acaatatagg
    22801 tattccttcc ttggtaacta gtccatcatt ggataataca accattctgt ctatgatgct
    22861 tttcagttct ctgatatttc cgggatagct gtaacttttt aaaaatttga ctgcttcatc
    22921 atcaatatga tgtatttcta tattgttttc cttttgagaa agattgagga aataggtcac
    22981 cagatcatca agatcttcct ttctttctct tagaggaggc acatttatca ctattgagct
    23041 aattctgtag aaaaaatctt cccttaatgc agatttcata acatcatcaa ccaaatttct
    23101 gtttgttgca gatataagct tgaagtcaat aagcttttcc atattgctgc ctaaacgctc
    23161 tacttttttt gtttccagaa cccttaacag ttttacctga gtggtcatat taatttctcc
    23221 gacttcgtca aggaataagg tgccgttgtt tgcaatttca aatttgcctt gctttgattt
    23281 catagccccg gtgaaagaac cctgttcgta accaaacaac tcagattcca aaagggattc
    23341 tgtgtaggag ctgcagttta ctgccacaaa gttatttgta cacctggaac tgcatatatg
    23401 tatgtacttt gctgctactt ccttgcctgt gccggattcg ccggttaata gaacattagc
    23461 atttgatttt gcaacctttt cacattgtga cagcattttg agatagcctt cattcttgct
    23521 gtgcagcatg aattcgccgg caaatttttc tcttaacttt attttgtcgt ttttatcatt
    23581 gtatagattt ataagtatgc ttgtaaggga ggttatttca ctgttcaaat ctatataaga
    23641 aacagctccc tggcttgcag atttactttc ctgtctatag gaaggagcag aggatgtaat
    23701 gattacatcg gtcaggggaa aagaacttgt gaaacttctg attgaaaatt cattggtaaa
    23761 caattcataa tctgcaataa aaaagctata ctcagcattg tcaagctcct tgtatgcatt
    23821 ttcagccccc aggcaatagc tatagtcaag ggcagctttt tttacaaatg agctgagtga
    23881 ggttatgtaa agcttatcat tacctgcaat taaaattttc atattgttca taaaatcctc
    23941 caatattgta ataattataa tacatgaaaa aatattcacg gtcaagaatt ttatttctct
    24001 atcaacgaat ttttcttatt aattttttac cgactgatgg tttgatagta ttgttttata
    24061 ttgagttatc aatacaaaaa gtattttaaa gcatgtccaa atcttggtat gatttttgct
    24121 agttaaataa caaacaacag aaagggggtt taaatgggca aaactattgt aactaacaat
    24181 tcaaaggttt acaacaagta taaagattta tatgagcttg tatatttgga ggatggtaat
    24241 tatacagatg ttttgtacaa tacaagagac tttatccaca aaggttttaa gcttttaacc
    24301 catcccatgg ccgggagcct taaaccaaat caaacaccat ataaatctgt cattgtagaa
    24361 atgtgcggta aaacagatta tgaatctgta gagcttattg aaaacagcat agacgcagca
    24421 ttaaagtttt taagcatcaa aaaaacacca gattggaatg aaaagatttt aaatgatttc
    24481 aagacagtgg atttatctat tattgaaaat gttgtaaaaa accctatgtt taatatgcac
    24541 aaataaaggt aatactaaaa tgaaaaaaat gaaggaggta caaatttgaa gttagaatta
    24601 ggaaagatta acatcaatga tgttcaattc ggcgacaaaa ctgaagtagt aaaaggcacg
    24661 ctttatgtta ataaggatga aataataaag cttgtcctcc aggacgaaag aataattaag
    24721 gctgatgttg aactggcgag acccggagaa tctgtaagaa tagctccggt caaagacgtt
    24781 attgaaccaa gggtaaaggt ttcagggagc ggagcaatgt ttcccggaac gattaacaag
    24841 gtaaaacagg ttggaagcgg cagaacacat gctttagtcg gtgctaccgt aataacatgc
    24901 ggcagaatag tcggcttcca ggaaggcttg attgacatgt caggtccggc agctaaatac
    24961 tgtccttttt cagaaacaaa taatgtggtt gttgtgatag aaccggctga aggtttggaa
    25021 actcacgact atgaagcagc aggaagaata gcaggactta aggttgcagc ctatgtaggc
    25081 gaagcaggaa gaaatgttga gcctgatgaa gtggttgtat atgaaactaa acctatttta
    25141 gagcaggcta aacaatatcc tgacctgcca aaaataggat atgtccatat gcttcaatca
    25201 caaggacttc tccatgacac atattattac ggtgttgatg caaaggttat ggttcctaca
    25261 tttatgtatc ctacagaaat aatggacggg gctattgtaa gtggcaactg tgttgcacca
    25321 tgtgacaagg taactacatt ccatcattta aacaatcctg taattgaaga cttgtacaag
    25381 cgccatggta aagacttaaa cttcatgggt gttgtcttga ccaacgaaaa tgtatttttg
    25441 gcagacaagg aaagatcatc agacatgact gctaaacttt gtcaatgctt tggatttgac
    25501 ggtgttctta taacagagga aggctatggc aatcctgata ccgaccttat gatgaactgc
    25561 aaaaaagtga ctcaaaatgg tgtaaaggta gtcttgatta cagatgaatt tcccggaaga
    25621 gacggaaaat cccagtcctt ggcggatgct gttgaagaag gcgacgcatt agtttcttgc
    25681 ggaaatggaa atgttatagt ttattttcct ccaatggaaa aggtaatagg cacactggat
    25741 ttcgttgaaa ccatgatagg cggatatgaa ggaagcttaa gggcggatgg aagcatagaa
    25801 gcagagcttc aaatagttat tgcatcaaca attgcaaatg gctacaacaa gctggctgcc
    25861 agaggatatt aaggagggcg tcatgagtaa agtaaaagta atacactata taaatcaatt
    25921 tttcgctcag gtaggcggcg aagaaatggc tgattatgag ccggaaatca gggaaggcat
    25981 agtaggtccc ggacttgcac ttcaacaagg gcttggtgag gactatgaag ttgttgctac
    26041 tgtagtatgt ggagacaact actttggaag tcatcttgaa gaagcgaaac aaagaatttt
    26101 ggatatgatt agaccctaca atccacagct gtttgtggca ggacctgcct ttaatgccgg
    26161 aagatacggc gttgcatgcg gaacaatagc aaaggctgtt gaagatgaat tcaacattcc
    26221 ggtagtaact ggcatgtatg ttgaaaatcc gggcgttgat atgttcagaa aagatctcta
    26281 catcatttca acagcaaatt cagctgcaga tatgagaaaa tcaatgccta aaatggctaa
    26341 gctggctaaa aagcttgtaa acaaagaaga aatattatca cctgccgaag aaggctatgt
    26401 tgaaagagga atccgcgtga actatttcaa tgaaagaaga ggctcagaca gagcagttga
    26461 tatgattctt caaaaaatca aaggccaacc atatgtgaca gaatatccca tgcctgcatt
    26521 tgacagagta gctcctaacc ctgccgttaa ggatatatca aaggtaaagg tagctgttgt
    26581 aacatcagga ggaatcgttc ctcaagggaa tcctgacaga atagaatcat caagtgcaac
    26641 aaaatacggt atttatgata ttaccggcat ggaagaaatg acaaaggaca agtttatgac
    26701 tatccacgga ggatacgaca gagcatatgt taccgaaaat ccaaacctgg ttgttccatt
    26761 agacgttctt cgcgaaatgg aaaaagaagg agtaattgga gaactggcaa attattttgt
    26821 tacaacaacc ggaacaggaa catcagtagg aaatgcaaaa ggatttggag aaaaattcag
    26881 caaaaagctt gttgaggatg gagttggagc tgttatcctc acatccacgt gaggtacctg
    26941 tacacgttgc ggcgcaacga tggtaaaaga aattgaaaga gccggcatac cggtagttca
    27001 tatgtgtaca gttgttccga tatcattaac tattggagca aacaggataa tacctgctgt
    27061 tggaattcct tatcctttag gtgatccaaa tttgggagaa gaaaagagca agaagctgag
    27121 aaggaccctt gttgaaagag ctttaagggc attacagaca gaaatatccg aacaaactgt
    27181 ttttgaagaa taagaaacca aaaatttaac caattaatgt gcttagcaca ttaattggtt
    27241 attacatcat gattttaagg aaggtataat atggataaag gcaaaggatt tgtgagtatt
    27301 aaaagagtta taataatggg cggtgctttc atcggtctct tgataggttc cggctttact
    27361 acaggccagg aaattatgca atactttgtt tcattcggaa acatgggtta tgcagcaata
    27421 attatgatgt ttgtattatt tgtgtatgtg ggaagcagtt ttgtagcagt tggctatgaa
    27481 caaaagtttg atgaacccaa aaatatttac caatattatt gcggtaaata tataggaaca
    27541 ttttttgatt atttttcggt aatattcctt ttcatgtctt tctgggtaat ggttgccggt
    27601 gcaggagctg catttaacca gcagtttgga cttcccaatt gggttggagg agttgtaatg
    27661 ggcacagcaa ccctcattac attatatttt ggatttagcc gcttagttga tattataggc
    27721 tcaattggtc ctgtagtttc ggttttggca atactactgg gtattgcagg aataatgatg
    27781 catcctgaag gtgtatctac cttttcaaca caggtgccgg cattggttga aagcggaact
    27841 gtgctgcagg cagggactaa ctggtttatg gcatgtgctt catatgtggg attctgcatg
    27901 atgtggctgg ctgttttcat gacaaacatg ggctctacgg caaacagcag aagagaaggc
    27961 atttatggag ctatttttgg ttcttcattg tattcagtcg ctgtattgct tctgatgtta
    28021 ggattaggtg caaacttaca agaagttgca ggtgcacaaa ttccggtatt gatacttgcg
    28081 aaaaaaatat cccctacttt agcgattatt tatactgcaa ttgttgttac ggaaatttat
    28141 acaacagcag gtcccctgct gtggacacct gtaaagcgtt ttgctcctga tgaaaaaagc
    28201 agccgctatc gtatgctgct gctggttctt ggagtgatcg gcatttttgt gggccttaga
    28261 gtttcttttg ccaagttaat caatgttatt tatgtaatca acgggtatgt gggattcatg
    28321 atgctctttt tgatgataat tacagacgta agagtgcgca tactcaaaaa ttacacccct
    28381 gccattgtaa ctgaactaaa gacaaagtaa caccaattaa aatatacgtt gaacactcaa
    28441 gaattatagc cgggagaaat cccggctttt ttagttctcc tgcacacgac ctaatgacct
    28501 gcccaatttt ttatatcgcg cttacctgtc tatttatttt ttttgtttta tatagtataa
    28561 tagttgaaaa ccataagtat gggaaatact cactaatatc cgtggatatc ttgtggtaaa
    28621 aagaatcagg agacttttat gaaatacaag atgatagtac ttgatttgga tggaactctt
    28681 ctaaatgacg aaaaaacaat aagtgataaa aatgcttata tacttgagga acttcataaa
    28741 caagggatac atattgttat tgcaaccggt agaaattatt atatggccag aattctcatt
    28801 gataggataa aaacaacaga gcctgtaatt ttagcgaata acggagcggt tgtaaggcgt
    28861 tatataaatg atgagtttat tgaaagcaat tatttggact tttcgatatt tgaaaaaata
    28921 tacgaattag gtataaagca gaatttaaat cccgtaattc atgttgatga ataccatcaa
    28981 ggatatgacc taatctacga atgtgaagac tacgaaaagg cctacctggg ttatataaag
    29041 aaggattacg gcagggcaag aaatacaaag tttcagaaag aagccattag taatatattg
    29101 tcggtctgct acttcaatga atatgaaaaa ctattaaaat ttaaaaaaga aattgaaaaa
    29161 atacccggtg aattcaatgt aatactcaat cagaacctta aaagcctttc tctattagag
    29221 ctactgaata ttaacggatg caaatggtgc gccttaaaaa aatatattga cggccttgga
    29281 atagataaaa gtcaagtggt tgccatagga gatgacaata acgacattga gcttattgca
    29341 aactcaggcc ttggaatagc catgaaaaat gcaaccggag gcttaaagga tgctgcggat
    29401 ttaatctcac gattttcaaa caatgaatcc ggagtatatt atgaattgtc agaaatattc
    29461 gaatttaaat aaaaatataa aagctttgta catattttca taactcctaa aatataatgt
    29521 attaatataa tgcaatattg aggaggaaat atgagaatta ttactgcaat agcttcaatt
    29581 cttataataa taggagcttt aaactggggt ttgtacggaa tatcaaaaat tgacatagtg
    29641 gaaagcttat tcggaggctg gaagaataaa tttggaagga ttgtttatat tttaattggt
    29701 attgccggat tatattcatt gttgtatgta atatttgctt gattttttac agacaaaaaa
    29761 catgccgctt atggcatgtt tttgtatgtc tgcaaaatgg atctacttaa atggtgccat
    29821 ttttgccatt ttgcccatca tgcggctgtg ctgccaattg cttgaaacta gttcagcaga
    29881 gcctaaagca atatttgcca atccaaatcc aataatcccg tttctgtaag attccggcaa
    29941 atttgtgttt ctcaaagcaa ctcctgttgc agtaacaacg gtccctaaaa ctgcgggaac
    30001 aatgccatct tttatttgca tatgaagcct cctttattta aatttgtatt attattattt
    30061 acattaaagt gtaaaataat tttataaatt aatggtaatt ttattgatta attcaatttt
    30121 cttaactata ataaaaaata aaatccgtta taaaaaggta aaatatgaaa ttatataata
    30181 aatattctga ttatttaaaa tcaaaatatg gagaaaaggt ttataagctt cctgtaaatc
    30241 ttccctgcac ctgtccaaac agggacggaa ctttaggtta cggaggctgt acattttgtg
    30301 ctgatgcagg aacgggtttt gaaatgcttg acagctcctt aagcgttaaa gaacagttaa
    30361 tcagaaatat ggcctatatc aaaaaaaaat acaaggctga aaaattcata gcttattttc
    30421 aaaattatac aaatacttat atggaaacgg aaaggttcaa aaattatata aatgatgcat
    30481 taatggatga cattgtggaa atagcagtat ctaccagacc tgactgcata tctgatgaaa
    30541 aacttaaatt tctttatgaa attaaagagt tccaaaatgt caacataaca attgaactgg
    30601 gtcttcagac agtaaactat cacactttgc aggctattaa cagaggccat agcttagcgg
    30661 aatttgttga tgccgtatta aggtgcaagg ggtataatct tgaaatttgt gcacacgtta
    30721 tactaaacct gccgggagat acaattctgg acacagctga gacggcaaaa atactatcgg
    30781 ttttaaaagt tgaccaggtt aaaattcatt ctctatatat aatggaaaat actataatgg
    30841 gagaattata taaaaataat gaaattacaa ttatatccaa ggacgaatat gtagaaagag
    30901 ttgttgtttt tcttgaacat ttacataagg atattgctgt acaaagattg gcgggtaggg
    30961 caccaaagga aaattccctg tttgtaaact ggggtatgag ctggtggaag ataaaggatg
    31021 aaattctgaa taaaatggag tctgaaaaca ggtatcaggg aagtaataat aaatttaaca
    31081 aattgtaaaa tccaaaaata aatagtgtaa acatttacaa catgtggtac aatatagttg
    31141 taatttactt ttataaaaaa gattggagtg tgattattat ggttcaattg gtttgtggtc
    31201 atagcggtac aggcaagaca agaaggatga ttgaaatggc aaacaaggct atagacaaaa
    31261 ttcacggcag catggttttt ttagaagcaa ataacagaca catattcgat ttggattaca
    31321 ggataagata tattaacact aaagagttcg ggctgactaa tgatgtagag ttccagggtt
    31381 tcgtatgcgg ggtaattgct actgattacg acatagaaca ggtatacata gacggtttat
    31441 acaaaatagc taagtcagga atagaaaaat tagaatctgt aatagaaagg ctagattatt
    31501 tatcaaaaac atttgatgtc aattttataa taagtgtaaa tcatgatatt gaagatattc
    31561 cagaaggatt gagagataga gttattgatt aatgcgtgcc gccggttaaa ccggcggctt
    31621 ttatattcct taattctgcg ttccctcgca tacagtatta actcttgcta aaaaagcttt
    31681 tttctgttag aataaatatt gtactaaaat tggaaagagt gatttcatga attattttaa
    31741 agcaaaaaac acaatattcg atgatttttt cgaacacaga gatatgctta taattcagca
    31801 tatgaacggg gacataaata agaaggaata cctggagctt aattataaat atatgcttga
    31861 aaaaaatatt aagccctttc agcgcattga cagctttgaa aaaggaatgt acaactatca
    31921 gtattacaat atgatggcaa aataccaccg catgatagca caggaaatca aggataaggg
    31981 aaagcacata agctattatt ctaaatatgt aaatgaagca gattattatt acaatgaaaa
    32041 ggataaaaca agtttccgcc tcttaaggtt tcttcaattc gaaaatgtgg aagcatattt
    32101 tataaaaatg gaatcatcaa ttctggaagg caagctttac gaaatagtct taaaggacta
    32161 tgaatatgcg gtacttcatt ctaaaagcct gtggcttctg gaagttttaa aaaaagaaaa
    32221 agtatttaca gaaaaaagga agagatcagt tattgattat tatgtaaatc aacggtatta
    32281 aggaagcaga catacattca tggcaaataa gcctgtgcgg taaatcaatt aaggaatgtc
    32341 ccaacagtat aagtatgact tagcattaaa aaacagctcg cgggctgttt ttttaataag
    32401 catttgcaat tatcttaata acatcatcaa gaggtgttcc taattcaata tcaaaagttc
    32461 cgctttgcag tcttgtatca agattgagtt caacgcagcg ggtaaggaat tcttccttgc
    32521 tttctattaa attgttattt agcagaattt ctcctatttt cgaagggtat gaaccttgag
    32581 gtatggtaat tgtagcaata attcctgaag gaatttcttc cgccggctcc tcttctaccg
    32641 gctcaggttc ttcctctgcc ggctcattta cgatgggcag gtccggtatc atttccttat
    32701 ctattcctct gctgaacaat aaatcgaatc tccaggccag tattaccgtc ataaccgcaa
    32761 ctacgacaat gatcaatccg tagtcggtta tgttatatat aaagtctcta attgcattaa
    32821 taatcttttt aagaattttc atttgtcacc tcttacctta ttgctctttt tattttagca
    32881 caaaaatccc gcaggtacaa taaccacatt taataattaa ttgaaaaatt tgcaaattac
    32941 atatacatta ataaaaattt tgggtataat gtatgggg
//
LOCUS       DAOPUT010000012        32619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_1285_length_32619_cov_19.243428, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000012 DAOPUT010000000
VERSION     DAOPUT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 32619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32619
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1285_length_32619_cov_19.243428"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(88..189)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            444..1715
                     /locus_tag="PKK75_02395"
     CDS             444..1715
                     /locus_tag="PKK75_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HNZ82478.1"
                     /translation="MQNLTVTNDGITINRNKHFFIISILPVTFLIMAFVFDKPANIFT
                     GLYEIILAPDILLTDYLAVGGFGAALFNVSSIVLINIFIIYKLNIEINGSIIAAIFTL
                     SGFAFFGKTIFNIWPMYLGGILYAKYKKITFDKIAVVIMFSTCLAPVVSKLAFSSGLP
                     LYRGIMLGVFFGITGGIIITPLSSNMSKLHGGHNLYNVGFTGGLMCTLICSLLKSFGI
                     NIEQQLVLSREYSTFFWNFLLIYFILLIVIGYLLNNKSFKGYVKIFGYTGKLLTDYTQ
                     LIGPGLTLVNMGIMGLICMSFVYINTGVFNGPIIGAVLTVVGFSAFGNHPGNSIPIMA
                     GVYLGAILKVWDIQTTPVIIAGIFGTTLAPIAGRYGVYAGVLAGFLHLSMVMNVGVVH
                     GGANLYNNGFAGGLVASILVPVFECFKREEE"
     gene            complement(1814..2005)
                     /locus_tag="PKK75_02400"
     CDS             complement(1814..2005)
                     /locus_tag="PKK75_02400"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HNZ82479.1"
                     /translation="MPIIQVEMLKGRTIEQKREMVKEITEVVTRTASCPKEAVKIIIR
                     EMEFENFSEGGVLRCDKQK"
     gene            complement(2034..3596)
                     /gene="tadA"
                     /locus_tag="PKK75_02405"
     CDS             complement(2034..3596)
                     /gene="tadA"
                     /locus_tag="PKK75_02405"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNZ82480.1"
                     /translation="MDEIFMKEALNEAYKAYEINEVPIGAVIVKDGEIVGRGFNQKET
                     QKDATLHAEMTTIKNACNNLGGWRLPGCTMYVTLEPCPMCAGAIVNSRIERLVIGARD
                     LKTGACGSVLDITNNKKLNHQPEVKFGVLEEECTEILQDFFSKLRKDKKKNQEDKMER
                     YPVLEFNLKKLYENTKIVTKLCQEYGIKVAGIIKGFGGIPEGAEEMAKGGCYQIGSSR
                     IEQLKALKDRGFEMPLLLVRIPMMCEIEDVVRYSDISLVSERETLNTLNEEAKKQNKT
                     YGVVLMYDLGDLREGVFLKEELIQLAQYVEYEMGNLYLEGIGSNLSCYGSVAPSNENL
                     SQLAEAATEIEEKLGRRLNIISGGGTTTLPLMVSGGVPKKINHLRIGEGINNTQDLPL
                     YWNTNIEGLDPDVFILKAQIVELNEKPTHPIGQLLVNAFGDLPHYDDRGIRKRAIVAL
                     GNQDLGDSSRLVPRDKDMQVMGASSDHTILDIHDCKKQYKLGDIVEFNILYQAMLMTS
                     LSDYVQKKIIRY"
     gene            3971..5044
                     /locus_tag="PKK75_02410"
     CDS             3971..5044
                     /locus_tag="PKK75_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ82481.1"
                     /translation="MKNIIKKTAALGLFALLAIHMNAAAYADVLDDIIGNSPVEDSYD
                     VESNEGENTNPGTLDARENGMAVLDSNTAKPSVSFQDIGSHWAKKWIESAYNLGFVTG
                     YKDGTFKPDNPVTRAEFSTMLNKAMKIEISKEFNFSDVSGSDWFNNEVKKSVAAGFFS
                     GYENNTFRPNNPITRQEAAKVVSGAITTGNIDGEGAALLNDYNTIQEWAKESVNTVYN
                     KGYILGYPDKTYLPARSLTRGEAAKIIFEIVDNENIEYGFNITNYNETYSSALVVGNL
                     NVLDSVENGNVYIKNVVVLGDIVISAKSVSSVVLTDVKARNIIVESENNPVKIVCNDN
                     VIIANTQIPSIATIQRLGLNINI"
     gene            complement(5178..6911)
                     /locus_tag="PKK75_02415"
     CDS             complement(5178..6911)
                     /locus_tag="PKK75_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82482.1"
                     /translation="MEKKNNFMPLLVYSIKEKKNWVFLSTVIIFLTTLLIPYILRADE
                     EFFIMLGIVELFILVFINCLVDNSFLHNESKLTYYRSKPVTLKRLIIINIVTNVVFTA
                     YLLALIVLSVVFQGVDYEILQVFKMLIPWLLAIILLVSLSSILSGNTLMAGAMTIFNF
                     CLPLIILLVTMFIFSILENIVRGFSADVLIDYFINNVYKLDYLYFTRYTDGSIDLAYM
                     LLLPAVLILISWLLNKFIKRRKNENTGFIVFDGFKYFVAVLACLIIPASFSISVGKYT
                     AMTNRIVISALLAVLSYYIIIAFIERSFRISRSSIKVFIAGMVVFTAITGGTVAVAGR
                     YENTVPLPEDVKMAYVGNSVWSANSVTDYLEGRSDETSSFSEWKRNRSIVVYEEIDNI
                     KLITDLHKEVLKDQTYYLDPDYYGMSNFVVAYWMKDGTILIRDYSLTPEQEQRPEHEQ
                     KNEIANKLFNSGDYKKQKFYYIYDEEYYSGRSLYAKLINLDNYSAAVEDIDLDNLREV
                     LKKDLVNLHFEKAAFAELLMNYFMHGKETSVKEQGYALEIYEKISDEDINYLDEIYLN
                     DIFINTLEYLK"
     gene            complement(6895..7785)
                     /locus_tag="PKK75_02420"
     CDS             complement(6895..7785)
                     /locus_tag="PKK75_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82483.1"
                     /translation="MLTIDDLSKKFSDFQVLDNVNINAEKSQIYGLVGSNGCGKTTTL
                     KHIMQIYKQDEGYIAFNGEEVKENSEILKDFYYVQDTLFFPYQYTLNKLFNYEKLMYN
                     MSEEKFEYLLKYFNIDKNKVLNKMSKGQKKQAAFVLSIAAGPKLLLLDEIVDGLDAVI
                     KRKFWDVLIKEVMDNEMTVIISSHDLKELDNICDKVGIMHEGRILKEENLEKMKEDIK
                     RVQFAVEGEFDAEQLKEFGLLQYVKIGSVYIVTMTGSTQDLEKYLKNTHTLLLFDKLS
                     MSLEEIFITELGGVGYGKEK"
     gene            complement(7769..8164)
                     /locus_tag="PKK75_02425"
     CDS             complement(7769..8164)
                     /locus_tag="PKK75_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ82484.1"
                     /translation="MIRIDNDNPKAIYEQIYDEIVRLIFSKSLKPDEKLPSVRELAST
                     IRINPNTIQKAYKYLEEDNYIYSVKGIGNFVKDADELKNLYVNNIKDELYNVIKSLKK
                     TGLKDERIVEMVKEFLNSDKKGGSDVNNR"
     gene            complement(8181..8723)
                     /locus_tag="PKK75_02430"
     CDS             complement(8181..8723)
                     /locus_tag="PKK75_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82485.1"
                     /translation="MKEIKIVLTQTNTVISKTLKFLSKKPYNHISISFEDDCSTMFSF
                     GRKITWFPLAGGFVKENIESGVFKMHPETKCKIYKFEVTDEDYDIISMRLNDFIAKPG
                     KYRYSFLNVFLIRLNIPYQREHYYVCSSFVSYLLKGIIPLQKEISLVIPDDYNNLNLK
                     PVYEGRLFEYVRNAVACMNA"
     gene            complement(8830..8906)
                     /locus_tag="PKK75_02435"
     tRNA            complement(8830..8906)
                     /locus_tag="PKK75_02435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8870..8872),aa:Arg,seq:cct)
     gene            complement(9097..10257)
                     /locus_tag="PKK75_02440"
     CDS             complement(9097..10257)
                     /locus_tag="PKK75_02440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNZ82486.1"
                     /translation="MKNLRRMLSLCLLIIVLTNTLVFAADINVDENIKGALLGDFESG
                     EILYEYNVNQQLAIGSISKLMTYTVMMDKVSQGEISLDDDVLISGHAAATEGSSFGIL
                     AGETIKLRTLVNGMLITSGNDCATAVAEHVGKTQDNFVNMMNAKASELGLLSASFINP
                     HGLPINDEETGQNYMSVSDLYKLVQHVLGTYPEILEITKQPELVVPERNFSKPATNPI
                     LGIVEGADGLKTGYTDKAGLCLVSTMPVKGSGQDFRLIGIILGAQTHDDRLNKTIELL
                     DYGKNNFKKSKLTDVSEVIDKVYISNSKTGKVNIFPASELSRVIKNEDVVTTKITYNE
                     SVKAPLSKGDIIGRISIFVNGEEIGQVEAAVNDNIEKANILVRLGRFFKDLF"
     gene            complement(10347..11270)
                     /locus_tag="PKK75_02445"
     CDS             complement(10347..11270)
                     /locus_tag="PKK75_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ82487.1"
                     /translation="MAIKQDFYYDSADKKTKIHAVKWIPEIETRAILQIAHGMFEYID
                     RYDYFARFMAENGILVAGNDHLGHGASVLKEEDRGFFSEGDGNMAVIKDMRKLQDILK
                     EEYEVPYFILGHSMGSFLVRQYVSIYHDMDGALIMGTGYPPYAKVMYGMIISRLMAVF
                     KGWRYRSRFINKLAVEKNNTYFEPSRTRSDWLTRDEEVVDAYINDKRINFIFTLNAFY
                     YMFKGMLYSYDKGYLDKIPGSLPLILLSGEKDPLGNFGKDVEKLYAVYKTKLTDVTMK
                     LYKDYRHEILNELEKDKVNEDILSWINKKMT"
     gene            complement(11315..12181)
                     /locus_tag="PKK75_02450"
     CDS             complement(11315..12181)
                     /locus_tag="PKK75_02450"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNZ82488.1"
                     /translation="MSIAIIYESEEWSNIYLRDYLMNKGFEVIFINFEEAVLNAAFFD
                     SAGLIVNRIFPSSYFRGHLKTYYKSIDFFRELNERGVPMINSYEALLYDFDKQLVCKT
                     LSENKINTPEIYCTTRDFSPDKIKYPCIVKPNSGGRSKYTIKVENGDELFEFLKTTPP
                     IDFIFQEYIKSVDNFTLRIEVIKGEVFSAVKRSTDESGISSYHRGAIYENIYKLNKHI
                     ENIVINTLKILNIKMGGIDVIVSSNGPYIIDVNATSNFSKTFVDFLKKNPLDKMGDLI
                     IEEYLKINQAVS"
     gene            complement(12222..12650)
                     /locus_tag="PKK75_02455"
     CDS             complement(12222..12650)
                     /locus_tag="PKK75_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HNZ82489.1"
                     /translation="MKDIELAKDLLKAQEKAIVIVKDGKVIFSSEGKGIKPVYTALNE
                     LKDRLQGSSAADRVVGRAAAMIYKHAGIKEISTSLISENALDILKNTSIDYEYDKLVP
                     FIKNRDRSGMCPIEKLSLEACDIDDLLLKISDFLKPLNGN"
     gene            complement(12793..14493)
                     /locus_tag="PKK75_02460"
     CDS             complement(12793..14493)
                     /locus_tag="PKK75_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ82490.1"
                     /translation="MGRKILIVGGVAGGASAAARLRRNSEDDRIIMFEKGPHVSFSNC
                     SLPYHLSGVIEDAEDLILMDPDLFLSRYNIEARINNEVLSIDRINKNVSVRNILTGEV
                     YTESYDKLILSPGASPIIENIKGLEKTNLFTVRNVVDIDNIKKFINKSTAKNIVVIGG
                     GFIGVETAENLRLAGYNVSIIIASRQILNTYDYDMVQIFHKEVFDKGINLLIGEKIEK
                     FENDKAYLKSGKIIDAHAVIMAKGVEPEIGLAREAGLEIGETGAIKVDKNYRTSDKNI
                     YAVGDAIEVYNALTHRPVKLALAGPAQKAARTAADHINNKNTFNRGFIGSSAIKVFDY
                     NGAATGLSESMIMSQKIKINYDIVRIILNDRVSIMPGAEPLHLKLIYEVPTGKILGAQ
                     AIGKGDAVKRVDVIATVIKFGGTLDDLKDLELCYAPPFSTAKDVVNYAGYVGSNLLNG
                     DFKQINVDSVRGLVESGAHIVDVRERYEYDEGHIKGALNIPLSEFRNRISEIPKDRPV
                     YLHCRTGQRSYNAVMALRNLGYENVHNITGSFLALSFYEYFNDITKDREKIVTEYNFL
                     "
     gene            complement(14505..14825)
                     /locus_tag="PKK75_02465"
     CDS             complement(14505..14825)
                     /locus_tag="PKK75_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNZ82491.1"
                     /translation="MFGLLQKTKINEININDVDDLMGKINLVDIREEYEYKSGSIKTA
                     KNIPMNQLLNNHDKYLQMGKKYYMFCLSGARSKRTVSYLAKIGYDVVNLSGGIGSYRG
                     KNRK"
     gene            complement(14925..16199)
                     /gene="serS"
                     /locus_tag="PKK75_02470"
     CDS             complement(14925..16199)
                     /gene="serS"
                     /locus_tag="PKK75_02470"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNZ82492.1"
                     /translation="MLDIKRIRNNPKEVEELLKRRNPELSLDKILELDKARREKLVEV
                     ENMKAEQNKVSKQVPLLKKEGKDVAGILQEMKELAEKVKTLDGQVKEIDEKIEEELLN
                     IPNTPNPDITVGTSDADNKEIRKWGEPRKFDFELKAHWDLGVDLDILDFERATKITGT
                     RFSMFKGAGARLERAITAYMLDKHTLDHGFTEISPPFMVNRDSMIGTGQLPKFEEDMF
                     KLSQRDYYLIPTAEVPVTNIYRNEVLEEKDLPMYLTAYTPCFRAEAGSAGRDTRGLIR
                     NHQFDKVEMVMYAKPEESYAQLEILTNFAEEILKGLGLPYRVVELCTGDIGFSSAKTY
                     DLEVWMPSYNRYVEISSCSNFEDFQARRANIKFRPEEKGRLEYIHTLNGSGLAVGRTF
                     AAILENYQNEDGSITIPEILIPYMRGIEKITL"
     misc_binding    complement(16397..16622)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(16662..17216)
                     /locus_tag="PKK75_02475"
     CDS             complement(16662..17216)
                     /locus_tag="PKK75_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ82493.1"
                     /translation="MVGEKIRNIRKSKNMTIVELSQLIDVTSGYISQIERDLISPSLA
                     VLKRLSNVLEVPLSVLFLEKSSDDVITIPHNERTKVKLNDVNVELEFITPILKSGDKK
                     TGFEIFLFKLNPGTWASDHGITHDFKEYIYVIQGEMECHVGEKIYTLSKGDSIIVPEN
                     NNHMFYNKNDDVTEGLCIISPSIH"
     gene            17482..19725
                     /locus_tag="PKK75_02480"
     CDS             17482..19725
                     /locus_tag="PKK75_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ82494.1"
                     /translation="MSDNSKYSEEIKYLDEIINLLKQKLEYEKSQLENQKSDLIESRR
                     EMWENTTHSSADFDKLTDLNQYLSALQAQTFTYAELAKRISKHEKMIESPYFARIDFT
                     EEGYDDTEKIYIGLFNLMDDKTHEIKVYDWRAPISSIYYRNEVGPVEYKAPAGLIKGN
                     VSLKRQFKISKGKMEYFFDSNINILDEMLMVALSKNMTSKMKTIVETIQKQQDLIIRD
                     LYNDLLVVQGVAGSGKSSVALHRIAYLMYQGLNLKLSANNIIVISPNPLFSKYISNVL
                     PELGEENINEYTFENIFHKLFENKLSMKTKSENFENIITAESEDQRNFLRTCDEFKGS
                     FVFSKIIDRFIDYYEHKLIPFEDVYFNGKIIEKHELSKAFLLSGKLDMPTGKKLKIIE
                     SRILEKVRENRIERRSKIEKAVNKLNNHEFETASFTRLLAARETQALAERVRKFTELD
                     SFEVYKKLFSDINIFKKISKNLDLPESIDEIISFTNKALSDPYNIPYADGTALMYMNI
                     KLEGCDLFSSIKQVVVDEAQDYYPIHYYILKNLYRYARFTIVGDVNQSVEKDSSFSLY
                     DDIVSIFNFEKSNKVFLTKSYRSSYEIARFSAKLLGDENFTEFFKRNEEEPALVKINN
                     SLRMQDEIVKTIEKYKSEGFSSIAMICKNRKEASDLYFKLSSKTKVKLIDYLDYDSVL
                     EGTLILPVYLAKGLEFDAVIVYEANDKQYNTKYDKKLLYIACTRALHRLSVFYTGKMT
                     RFLEDLR"
     gene            complement(19991..20061)
                     /locus_tag="PKK75_02485"
     tRNA            complement(19991..20061)
                     /locus_tag="PKK75_02485"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20027..20029),aa:Gly,seq:ccc)
     gene            complement(20069..20141)
                     /locus_tag="PKK75_02490"
     tRNA            complement(20069..20141)
                     /locus_tag="PKK75_02490"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20106..20108),aa:Ala,seq:ggc)
     gene            complement(20249..20321)
                     /locus_tag="PKK75_02495"
     tRNA            complement(20249..20321)
                     /locus_tag="PKK75_02495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20286..20288),aa:Thr,seq:cgt)
     gene            20481..20747
                     /locus_tag="PKK75_02500"
     CDS             20481..20747
                     /locus_tag="PKK75_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82495.1"
                     /translation="MYNDNGMNNTVYSSNRYDMYDRRFDPEYDVMYDRRFDPEYDMMY
                     DRRYDRRYDMDFYDPIRYYQFPYCDRYGRCENPIWWLFWPLFFL"
     gene            complement(20790..21350)
                     /gene="pyrE"
                     /locus_tag="PKK75_02505"
     CDS             complement(20790..21350)
                     /gene="pyrE"
                     /locus_tag="PKK75_02505"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNZ82496.1"
                     /translation="MIDVVEILKECGALLEGHFLLSSGKHSNRYCQCAKLLQYPDKAE
                     KVISLVRDKVAPLDLNVIVGPAMGGITAAYELGRQLGIKAIFTERENNVMTLRRGFEI
                     KPGDRVLIMEDVVTTGKSSGETAKVLESLGGIVVGIGCIVDRKASEIPLPIYSSVELF
                     FETYDEENCPLCERGSKPVKPGSRKF"
     gene            complement(21464..22366)
                     /locus_tag="PKK75_02510"
     CDS             complement(21464..22366)
                     /locus_tag="PKK75_02510"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNZ82497.1"
                     /translation="MANLETNAMGFKFKNPILAASGTFGFGEEFSQIYDISILGGICS
                     KGLTLTPRSGNEGIRLWETPMGLMNSIGLENPGVDKFISEELPRMKMYDTIIFANLGG
                     HCEQDYFEGIYTLNDTNVDGVELNISCPNVKSGGMAFGVKSSVAYEIVSKVRKICKKP
                     IMVKLSPNAENISDMATACEAAGADAISLINTVQAMAVDIKNRKAVFDNIYAGLSGPC
                     VKPIALRMVHQAAKMVKIPICGLGGIMNAADIVEFIMAGASLVQIGTANFIKPDIAID
                     IINDLEEFLDNENLKSLDEIRGII"
     gene            complement(22376..23104)
                     /locus_tag="PKK75_02515"
     CDS             complement(22376..23104)
                     /locus_tag="PKK75_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNZ82498.1"
                     /translation="MKVLLAPVYENKQISNNIYRLSAEFESEMKPGQFFMLKTTDNSF
                     LLPRPVSINDVNGKLVSFLYRIEGQGTKRISSLRKNDQLQLFGPLGNGFEPDKLKGKT
                     AVIGGGIGIAPLLYLCKTLGKNADVYLGYRDLDNMYITDEFKSFAAACVIVTEDGSFG
                     EKGFVTDYVPFEKYDVVAACGPEIMMKRVAAICRDKNIKCYLSLERRMACGMGACLGC
                     TVKTKTGNKRACREGPVFDSEELL"
     gene            complement(23127..23552)
                     /locus_tag="PKK75_02520"
     CDS             complement(23127..23552)
                     /locus_tag="PKK75_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNZ82499.1"
                     /translation="MLNIDSVEKGLVIDHIKAGKAMEIYHYLNLDKMDCSVAIIKNAK
                     SKKYGKKDIIKVENNIDMDLKVLGFIDPNITINVIDNSVIIEKINLELPQEVVNVVKC
                     KNPRCITSVEQEIVHKFRLNDREKEIYRCVYCDTAYDKK"
     gene            complement(23552..24475)
                     /gene="pyrB"
                     /locus_tag="PKK75_02525"
     CDS             complement(23552..24475)
                     /gene="pyrB"
                     /locus_tag="PKK75_02525"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNZ82500.1"
                     /translation="MLKGRSIIEPMDLSLEELDEILNLANEIIQNPASYADVCKGKVL
                     ATLFYEPSTRTRLSFESAMLRLGGSVTGFSSAESSSAAKGESVADTIRTVACYADIAA
                     MRHPKEGAPMVAAMKANIPVINAGDGGHQHPTQTLTDLLTIKTLKGKLNDFTIGLCGD
                     LKFGRTVHSLIKALSRYENVKFVLISPEELKIPDYIREEILIKNNIEFTEVERLDNVI
                     GQLDVLYMTRVQKERFFNEADYIRLKDSYIVDNKKMANAKKDMILMHPLPRVNEISVE
                     VDDDPRAAYFTQTKCGMYIRMALIMKLLGVE"
     gene            complement(24849..25073)
                     /locus_tag="PKK75_02530"
     CDS             complement(24849..25073)
                     /locus_tag="PKK75_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNZ82501.1"
                     /translation="MPMKLNVGDVVKLKKKHPCGGYDWEILRIGADFRIKCTTCDRQV
                     WLPRSEVERRITKIVYQADKQPDNEPSDLQ"
     gene            complement(25085..25672)
                     /gene="yedF"
                     /locus_tag="PKK75_02535"
     CDS             complement(25085..25672)
                     /gene="yedF"
                     /locus_tag="PKK75_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HNZ82502.1"
                     /translation="MKIVDARKKACPQPVIMTKKEVDGGAEEITVIVDNETAKINVTK
                     FGTQLKYTVTAEDKDDGTYIVLKKSGENKEQTEIISEIKNKNAGYFITSDKLGKGSDD
                     LGRILMKSFLYTLSETKPYPDFLIFLNSGVKLTTEGSESVDDLMKLKEGGVKIVSCGT
                     CLDFFEIKDKLRVGSISNMYDIVDMISSSTNTITI"
     gene            complement(25682..26326)
                     /locus_tag="PKK75_02540"
     CDS             complement(25682..26326)
                     /locus_tag="PKK75_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNZ82503.1"
                     /translation="MIVDVIILVFAGYLCFMGYRKGFILTVFDTLGLVISFFLSKEFY
                     NFAENFLLNNTKLFVKLHDYFETKLSMSAVSNTSKIPVELQKFVNNIMASEASDTFAV
                     FVDNISLLIIRSISFVVTFLAIYVILLILTAFINTVMKLPFLNLTNRLFGAFMGVLKS
                     VILLYLIFALAAPVLSFMQDKPFAQEVLKSESSKIFYDNNIILNYLSYKGFYEN"
     gene            complement(26345..27397)
                     /locus_tag="PKK75_02545"
     CDS             complement(26345..27397)
                     /locus_tag="PKK75_02545"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HNZ82504.1"
                     /translation="MKKIILFIVIILIFIVALLYSPYFKELTKTLDEKKVYKIEGIKT
                     TEISTLEGFEFFNRGIISYNNQKTIYTDFDNKILWENQNTEFSNRVFVTDNYIFRQAN
                     NNIKVLDKNNQEFVIAEIEGNIINVSRENNKTGMIVKGNGQTLYIMDDNNEIVVDNKE
                     FQDTITGISISDKSDAYSVITLVFENGAFVNNIYYFIEDVELWSSIISKEILIKTKVV
                     NNNVIVIGTENIYFYNDNGKLMWKNKIFNKVLDYEISQGMINILFEKDQAAELLSYNF
                     EGKVMEIKTAPAGSKKLKIADNKTLTYNNNTIYLLHGNKTDKLFEDTESFEDFMIEGN
                     NIYILFKNKIIEGQIK"
     gene            complement(27530..28669)
                     /locus_tag="PKK75_02550"
     CDS             complement(27530..28669)
                     /locus_tag="PKK75_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ82505.1"
                     /translation="MIYLDNAATTFPKPSSVYENVMKAMTVYGANPGRGSHKMAIEGA
                     RVIYETRELLAELFNLNDPMKVIFTFNATDSLNTAIKGVLNSGDHVITTEMEHNSVLR
                     PIKELEKSGVENTIVKCSSDGTMDLKDLENAINNNTRLIVTTHVSNLTGTIFPAEEIG
                     KICKKHNIPYLLDASQSAGVLDIDMNKFNISFLAVPGHKGLLGPQGTGALLINSDIEI
                     KHLKEGGTGSQSSSLDHPNFYPDKLEAGTHNLPGIAGLNAGVRYILNRGIKSIYSHEK
                     NLLETFIIELRKNPKIKLYGPESIEHRSGVLPVNIEGMDSSEVANILDSEYNIAVRPG
                     LHCAPLAHKAIGTEKLGAVRFSLGPFNKTADIYAAVKALNEIAAR"
     gene            complement(28733..29986)
                     /locus_tag="PKK75_02555"
     CDS             complement(28733..29986)
                     /locus_tag="PKK75_02555"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNZ82506.1"
                     /translation="MIVKNGRIIDPASKIDEIADILIENDIIKRIDKNIKVDGERVIN
                     AEGRIIAPGLIDIHVHFREPGFEYKEDIISGSKAAARGGFTTVVCMANTAPAVDNIET
                     LKYINNIIKHSSINVLQAAAVTKGLKGTEIVNMEELKNNGAAGFSDDGFPIMDTNILL
                     NAMLEAKRLNLPISLHEEDPRLIDSPGINEGIISKKLNIKGAPQLAEDVMVARDCMIA
                     LKTGAKVNFQHISSGLSVDIIRWAKLMGANITAEATPHHFSMTEDDILEFRTNAKMNP
                     PLRTENDRMKIIAGLKDDTIDIIATDHAPHSKEEKDRELSKAPSGIVGLETALSVGIT
                     YLVKNNHLTISQLLEKMTVNPARLYNLESGIKEGNKCDLVIFDIDESWNVDFISKSRN
                     SPFLGRKLWGKVKYTICKGRIVYKD"
     gene            complement(30292..32103)
                     /locus_tag="PKK75_02560"
     CDS             complement(30292..32103)
                     /locus_tag="PKK75_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNZ82507.1"
                     /translation="MDKVNLTINGINVSVPKDYTVLLAAKEAGIDIPTLCYLKDINEI
                     AACRLCIAEVDIKGVPMRNLPATCVLQVQEGMNVKTNTKRVRNAVRMNLELILANHNR
                     ECLTCLRNQTCELQKLCDEMNISDIKYEGEKREAKIDNLSYSIVRDTSKCILCGRCVS
                     TCKEVQGIGILDFTKRGFVTEVAPAFNYSMKDVPCVYCGQCIQACPVAALRERTYVDD
                     VWDAIDDPEKIVVVQTAPGVRASLGEEFGLPVGTDVTGRMVAALKRIGFDKVFDTNFS
                     ADLTILEEGTELLDRVKNGGKLPMITSCSPGWIRFCELNYPEFLDNLSTCKSPQQMFG
                     AMVKSYYAQKAGIDPKKIFSVSIMPCTSKKTEASRPEMEVDGLRDVDVSLTTRELGDM
                     IKQARINFVDLPDEEADSILGQYTGAAAIFGTTGGVMEAALRTVADILTGKDLEDIEY
                     QGVRGLDGVKEASVVLPIDGKDTEIKIAVAHGTANAAKVLDAVNKGEKEYHFIEVMAC
                     PGGCVHGGGQSHVSAKKRLDVNPKAKRADALYELDKSLPLRKSHKNPEVIKLYEEFLK
                     EPNGHISHKYLHTHYYKRNSYEIAQQEEYDKLMEKLK"
     gene            complement(32117..>32619)
                     /locus_tag="PKK75_02565"
     CDS             complement(32117..>32619)
                     /locus_tag="PKK75_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNZ82508.1"
                     /translation="IVMDEDNCMVDIARFFLDFTVDESCGKCTPCREGTKRMLEMLNK
                     IAEGKGTMEDLDKLEKLAINIKETSLCGLGQTAPNPVLSTLKYFRDEYEAHVKEKRCP
                     AGVCQSLLKYMITMDCKGCTKCARLCPAGAIEGKVKEVHVINQDKCIKCGACMDACTF
                     HAIIRK"
BASE COUNT         9495 a         6585 c         5148 g        11391 t
ORIGIN      
        1 tgcttaataa ttttttcatt aacatcctcc taattttata aactttgcac caagaaagta
       61 aaaatgcgat agtgtaacta atcgcatcaa tagaataata atatcaatag tcaaacaatt
      121 tacggttgtt tggtagaaac tttggaacca tattatccac attatattga tgcagtttat
      181 atttaattaa tataattata acttaagcta tgacaataat caacaaatta tttttgaaat
      241 taatcgtgtc cggcaagtga ccgctgctat gtattccctt atatatggct gtatctgcaa
      301 agtttttcac aggatgatga agtaatgaaa tagcattttc cacaattttc aaaggttaaa
      361 tatttagtgt tgaatattgt ctatttatat gctaaaatat tatcaaagta aagtgctatc
      421 attggcagac aggagaaaat ttcatgcaaa atttaactgt aactaatgac ggaattacta
      481 taaacagaaa taaacatttt tttataatat caatattacc tgttactttt ttaataatgg
      541 cttttgtttt tgacaaacct gcaaatattt ttacaggttt atacgaaata attcttgcac
      601 ctgatatttt actgacagat tacctggcag tggggggttt cggagctgca ttgtttaatg
      661 tgtcttcaat agtgcttata aatattttta tcatatataa attaaatata gaaatcaatg
      721 gttcgattat tgccgccata tttactttat ccggttttgc cttctttggc aaaaccattt
      781 ttaatatatg gccaatgtat ttaggcggga ttttatatgc caaatacaaa aaaattacct
      841 ttgataaaat tgctgtagta ataatgtttt caacatgcct tgcacctgta gtgagcaagc
      901 ttgcattttc ttcaggatta cccttgtatc gcggaattat gcttggtgtg ttttttggaa
      961 taacaggcgg aattataatc acacctctct catctaatat gtccaagctg catggaggac
     1021 acaacttata caacgtaggg tttaccggag gactcatgtg tactttaata tgttcattat
     1081 tgaaaagctt tggaataaat atagagcagc agcttgtcct ttcaagggaa tacagtacct
     1141 ttttttggaa cttcttactg atatatttta ttttgttaat tgtaatagga tatttattaa
     1201 acaataaatc atttaaggga tacgtgaaaa tattcggcta tacggggaaa ttattaacag
     1261 attatactca attgattggt ccgggtctta ctcttgtaaa catgggcatt atgggtttaa
     1321 tatgcatgtc ttttgtatat attaacaccg gtgtatttaa cggtcccata ataggagctg
     1381 tccttactgt tgtaggtttc tcggcttttg gcaaccatcc cggaaactca atccccatta
     1441 tggccggagt ttacctgggt gctatcttaa aagtgtggga tatacagaca acaccggtta
     1501 ttattgcagg aatatttggt actacattag caccgatagc cggcaggtac ggtgtttatg
     1561 caggtgtttt agctggtttt ctgcatcttt caatggtaat gaatgtggga gttgttcatg
     1621 gaggtgcaaa tctttacaac aatggatttg caggaggttt ggtagcttcc atacttgttc
     1681 ctgtgtttga atgctttaaa agagaagagg aataaattta ttccaaaatc ccacctggat
     1741 aaaaaaatga accgttgaaa acatcaacgg ttcatgttaa tttccattat ttcgaagctt
     1801 aaaacagtaa attttatttt tgcttatcgc atctgagaac tccgccttct gagaagtttt
     1861 caaactccat ttctcttatt attattttta cagcttcctt tggacaggaa gcggttcttg
     1921 ttacaacttc tgttatttcc ttaaccattt ctcttttttg ttcaattgtc ctgcccttaa
     1981 gcatttccac ctgaattatt ggcatatttt cacctccttg tcttatgaat gatttaatac
     2041 ctgattattt tcttttgtac ataatctgac aaagatgtca taagcatggc ctggtacaaa
     2101 atattgaatt ccactatatc gcctaattta tactgttttt tgcagtcatg aatatccagt
     2161 atggtatggt cgctgctggc tcccattacc tgcatatcct tgtcccttgg aacaagtctt
     2221 gatgaatccc ccaagtcctg gttccccaag gcgacaattg ctctttttct tattcctctg
     2281 tcatcatagt gaggcaggtc cccgaaagca ttcaccagga gctgtccaat cgggtgggtt
     2341 ggtttttcat tcaactccac aatttgagct ttcaaaataa acacatccgg atctaatcct
     2401 tcaatatttg tgttccagta aaggggtaaa tcctgtgtgt tatttattcc ttccccaata
     2461 cgaaggtgat ttattttctt cggtactcct ccgcttacca tgagaggcag agttgttgtt
     2521 ccccctcctg atatgatgtt taacctgcgt cccagttttt cctcaatttc tgtggcagct
     2581 tccgccaatt gactaaggtt ttcattggaa ggtgcaactg agccatagca gcttaaattt
     2641 gaacctattc cttcaagata cagattaccc atttcatatt ccacatactg tgctaattga
     2701 ataagttctt cttttaagaa tactccttct cttaagtctc ccaaatcata catcagaaca
     2761 acgccgtatg ttttattttg tttcttagct tcctcattta acgtgttcaa ggtttccctt
     2821 tcagaaacca gtgaaatgtc gctgtatcta acaacatctt caatttcaca catcatgggg
     2881 attctgacca ataaaagagg catttcaaat ccccggtcct tcaaagcttt aagctgctca
     2941 attctggagc tgcctatttg ataacagccg ccctttgcca tttcttccgc gccttccgga
     3001 attcctccga atcctttaat tatgcctgct accttaatcc cgtactcctg gcatagtttt
     3061 gtaactatct tggtgttctc atagagtttt ttcagattaa attccagtac agggtatctt
     3121 tccattttat cctcctgatt tttcttttta tctttacgaa gctttgagaa aaaatcttgt
     3181 aatatttcag tgcattcttc ctccagtacg ccgaatttaa cctccggctg atggtttagc
     3241 tttttattgt tagttatatc caaaacagac ccacaggccc cggtttttaa atccctggct
     3301 cctattacca gtctttcaat tcttgaattg actattgcgc cggcacacat ggggcagggc
     3361 tccagggtta catacatggt gcagccggga agccgccatc ctcccaagtt attgcaggca
     3421 ttctttatgg ttgtcatttc agcatggagg gtggcatcct tttgtgtttc tttttgattg
     3481 aaccccctgc ctacaatttc tccatctttt actattacgg caccaatggg aacctcattt
     3541 atttcatatg ccttgtaagc ctcatttaag gcttccttca tgaatatttc atccataaca
     3601 ttttcctgtt tatttttatt cttaattata gtttactttc ttcattcaag tcaatagttt
     3661 atgtagaaag ccttataaaa tcagcggaaa ttatctgcaa tttaaaggcg cctgttcttt
     3721 ccccttatat tacctgttta ctttaggttt aaaaaactat aaaattatta taagctcaag
     3781 tgcccccatc cttggatttt gacttatcaa aaaacctctc cttaaaagaa tgaagcaaag
     3841 ctctttaaag ggattacccc tggcggcatg ggaatgccac agtgcttgga ttacccctgc
     3901 agttaaagaa atatcactgt atttggaaca aatacagttt taagtcagtc taataaaaga
     3961 aggaggtaat atgaagaata ttataaaaaa aacagccgcc cttggtcttt ttgccctgct
     4021 ggcaatacac atgaatgctg cagcttacgc tgatgttctg gatgatatta ttggaaacag
     4081 tccggtcgaa gattcatatg atgttgaaag caatgaaggc gaaaatacaa atcccggtac
     4141 attggacgcc agggaaaacg gcatggcagt attggactca aatacggcaa aaccatctgt
     4201 ttcattccag gacattggca gtcactgggc aaaaaaatgg attgaaagcg catataattt
     4261 aggatttgta accggatata aagatggaac attcaagccg gacaatcctg ttacaagggc
     4321 tgaattttca acaatgctaa ataaagcaat gaaaattgaa ataagcaaag aatttaactt
     4381 ttccgatgtg agcgggagcg actggtttaa caatgaagtg aaaaaatctg tggctgcagg
     4441 gtttttttcc ggttatgaaa ataatacatt caggcccaac aatccaataa cccgccagga
     4501 ggctgcaaag gttgtgtcag gtgctataac aactggaaat atcgacggtg aaggtgctgc
     4561 gttgttaaat gattataata ctattcagga atgggcaaag gaaagtgtta atactgttta
     4621 caacaaagga tacatactag gctatccaga caaaacctac ctgcctgcca ggtcattaac
     4681 caggggagaa gcagcgaaaa ttatttttga gatagttgac aatgaaaata ttgaatacgg
     4741 atttaatatt acaaattaca atgaaactta cagcagcgct cttgtggttg ggaatttaaa
     4801 cgttttggat tccgttgaga acggaaatgt ttatattaaa aatgttgtgg tattaggaga
     4861 cattgttatt tcagccaaaa gtgtcagttc cgtcgtactc accgatgtta aggcaagaaa
     4921 cataatagtt gaaagcgaaa acaatcctgt aaaaatcgtg tgcaatgaca acgtgataat
     4981 tgcaaatact caaataccct caattgcaac cattcaaaga ttaggactaa atatcaacat
     5041 ctagaggatt gggacacacc ttagctatag ggaaattccc tgaatgcaag atgaatgaac
     5101 aagcataaaa aagattcttc attgggaaga atcttttttt ttaattaagg cttcatcaaa
     5161 aaaccggcac taaaaaatta cttcaggtat tccagggtgt ttatgaaaat atcatttaaa
     5221 tatatttcgt ctaaataatt tatatcttca tctgatattt tttcatatat ttctaaagcg
     5281 tatccctgtt cctttaccga agtttccttg ccatgcatga aataattcat aagcaactcc
     5341 gcaaaggccg ccttttcaaa atgcaggttt actaaatcct ttttaagcac ctctcttaaa
     5401 ttatccaagt ctatatcttc aacagcggca gaataattat ctaaattaat taacttggca
     5461 tataagctcc ttccgctgta gtattcctca tcatagatgt aataaaattt ctgtttttta
     5521 tagtcgccgg agttaaacag tttatttgct atctcatttt tttgttcatg ctcaggtctt
     5581 tgctcctgct ccggagttaa actgtaatcc cttataagta ttgttccgtc cttcatccag
     5641 tatgcaacaa caaaattgct catcccgtaa taatcagggt ctagataata tgtttggtcc
     5701 tttaatactt ccttatgaag atccgtaatg agcttaatgt tgtcaatctc ctcataaacc
     5761 actatgctgc gatttctttt ccattctgaa aaggatgaag tctcatcgga cctgccctcc
     5821 aaataatcag ttacagagtt tgcagaccag acactattgc ccacgtatgc cattttaaca
     5881 tcttccggca agggaaccgt attttcatac cggccggcaa cagctactgt accccctgtt
     5941 atggcggtaa atacaaccat gcctgctatg aaaactttta ttgatgagcg tgatattcta
     6001 aatgatcttt caataaatgc aattattata tagtaggata ataccgccaa taaagctgaa
     6061 attactattc tgtttgtcat agcggtgtac tttcccacgc ttattgaaaa tgacgccggt
     6121 ataatcaggc atgcaagcac cgccacaaag tatttaaacc cgtcaaatac tataaatccg
     6181 gtattttcgt tttttcttct ttttataaat ttattaagaa gccaggaaat aagtatcagg
     6241 acggcaggca gcaaaagcat gtatgccaag tctatggagc cgtccgtata tcttgtgaaa
     6301 tacagataat caagcttata tacattgttg ataaagtagt caattaaaac atccgcgctg
     6361 aatcccctta ctatattttc caggattgag aatatgaaca ttgtaaccaa aagtattatc
     6421 aatggaaggc aaaaattaaa tatagtcatt gctcccgcca tcaatgtatt tcctgataat
     6481 atggatgaca gggataccag cagtattatt gccaaaagcc agggaatcag cattttaaac
     6541 acttggagta tttcatagtc aactccctga aatactactg acaggactat gagagcaagc
     6601 agataagccg taaaaaccac atttgtcact atgtttatta tgatcagtct ttttaatgta
     6661 acaggtttcg acctgtagta agtcagcttt gattcattgt ggagaaaact gttgtcaacc
     6721 aggcagttta taaaaaccaa aataaataac tcaacaattc cgagcattat gaaaaattct
     6781 tcatctgctc tcaaaatgta gggtattaaa agtgttgtta agaaaattat aacagttgat
     6841 aaaaaaaccc agtttttctt ttccttaatt gaatatacta ataatggcat aaagttattt
     6901 ttcttttcca taacccacac ctcccaattc cgtgataaat atttcttcaa gactcattga
     6961 cagcttatca aacaagagaa gagtgtgtgt atttttaaga tatttttcaa ggtcttgcgt
     7021 gcttccagtc atcgttacaa tatatacgct gcctattttt acatattgaa gcaagccaaa
     7081 ttctttaagc tgttcagcat caaattcacc ctccacggca aactgaactc ttttaatgtc
     7141 ctctttcatc ttttcaaggt tttcttcttt taaaattctg ccttcatgca ttattccaac
     7201 cttgtcgcaa atattatcca gttctttcag gtcatgggag gaaattatga cagtcatctc
     7261 attatccatt acctctttta taagtacatc ccaaaacttt ctttttatta ctgcatccag
     7321 gccgtctact atttcatcaa gcaaaaggag ttttggcccg gctgctattg acaatacaaa
     7381 tgctgcctgc ttcttctggc cctttgacat tttattcaat actttgtttt tatctatgtt
     7441 aaagtatttc agcaggtatt caaatttttc ttcagacata ttatacataa gtttttcata
     7501 attaaataat ttgtttaatg tatactgata gggaaaaaac aatgtatcct gaacataata
     7561 aaagtccttt aatatttccg agttttcttt aacctcttcc ccgttaaagg ctatgtaacc
     7621 ttcatcttgc ttgtaaattt gcattatatg ctttaatgtt gtggtttttc cgcagccgtt
     7681 ggagcctacc aaaccataaa tctggctttt ttcggcattt atgtttacat tgtccaaaac
     7741 ctgaaagtcg ctgaatttct tgcttaaatc atctattgtt aacatcgctg cctccttttt
     7801 tgtccgaatt taaaaattct tttaccattt ctacaattct ttcatccttc agcccggttt
     7861 tcttcaggga cttaataaca ttatacaatt catcctttat attatttaca tataaattct
     7921 ttaattcatc agcatccttt acaaagtttc caataccctt cacgctgtag atataattgt
     7981 cttcttccag gtacttatat gccttttgga tggtgttggg attgattctt atagttgaag
     8041 caagctctct tacagaaggc aatttttcat cgggtttcaa agacttagaa aatataagcc
     8101 taactatttc atcataaatt tgttcataaa tggcttttgg attatcatta tcaattcgaa
     8161 tcaatctttt tcacccccgg ttatgcattc atgcaggcaa ccgcatttct cacatattca
     8221 aacaatcggc cttcatatac cggctttaga ttcaagttat tgtaatcatc cgggataaca
     8281 agagatattt ccttctgtaa gggtattatt cctttcagaa gataagatac aaagctggag
     8341 cagacataat agtgttctct ttgatagggt atgtttaagc gaattaaaaa aacatttaaa
     8401 aaactatatc tgtattttcc cggtttggct ataaaatcat tcaaccgcat ggaaataata
     8461 tcataatctt catcagttac ttcaaattta tatattttac atttagtttc aggatgcatt
     8521 ttaaaaacgc cgctttctat attctctttt acaaatcccc cagctaaggg aaaccaggtg
     8581 atctttcttc cgaagctgaa cattgtcgag cagtcatcct caaaggatat cgaaatatga
     8641 ttatatggtt ttttggaaag gaattttagt gttttgctta taactgtatt cgtttgggtt
     8701 aaaacaattt ttatctcctt cataactgtc ccctaaaaat ttcgttacaa gtgtattagt
     8761 acacttagta cagtaattgc attatatatc atggcgcacc cgatgtcaag aagaaattcc
     8821 gcaaaaaaat ggtgcacctg agaggaatcg aacccccgac acatgcctta ggagggcact
     8881 gctctatcct tctgagctac agatgcacgt tattttaaaa tgttacacta ttttttttat
     8941 tctgtcaata ttattactca atatctccca tatccctatg tgcaacttta tttaaagcaa
     9001 aattttctat ccataattac tcttttaaac aaaaaagttt agataataaa cttttcagat
     9061 tatttataat tcctattttc atcctgagaa ttatagttaa aataaatctt taaaaaacct
     9121 tcctaacctt accagaatat ttgctttttc tatattatca ttcacagcag cttcaacctg
     9181 tccaatttct tctccattga caaaaatact tattcttcct attatatcac cttttgacaa
     9241 gggggcttta acagattcat tgtaggttat tttcgttgta accacatctt cattttttat
     9301 gaccctgctt aattctgatg caggaaaaat attcaccttt ccggtttttg aattggatat
     9361 ataaaccttg tctatcactt cagatacgtc ggttaacttt gattttttaa agttgttttt
     9421 tccgtaatcc aatagctcaa tagttttatt taacctatca tcatgggttt gggcccccaa
     9481 aattattcct atgagcctga aatcctgacc acttcctttt acaggcatgg tggataccaa
     9541 acagagacct gccttgtccg tatatccggt ttttaaaccg tcagcccctt caacaatacc
     9601 taatatagga tttgttgcag gcttgctgaa atttctttca ggaacaacca gctcaggctg
     9661 ttttgttatt tccaaaattt caggatatgt gcctaaaaca tgctgcacca gcttgtacaa
     9721 atctgaaaca gacatatagt tctgtcctgt ttcctcgtca tttataggca ggccgtgagg
     9781 attaataaag ctagcagaca aaaggccaag ctctgaagcc ttggcattca tcatattgac
     9841 aaaattatcc tgagttttcc caacatgctc tgccactgca gtggcacagt catttcctga
     9901 ggttataagc attccgttta ccaaggttct cagtttaatt gtctcccctg ctaaaattcc
     9961 aaaactgctt ccttccgtag cagcggcatg gccgcttatc aacacatcgt catcaagaga
    10021 tatctcacct tgagaaacct tatccatcat tactgtgtag gtcatcagct ttgaaatgct
    10081 tccgatggca agctgctggt taacattgta ttcatagagt atttcaccgc tttcaaaatc
    10141 ccccagcagg gcgcccttaa tattttcatc tacatttata tctgcagcaa aaacaagagt
    10201 gtttgttagt acaatgatta gtaaacacag ggaaagcatt cttctgagat tcttcataaa
    10261 aacctcctac catctatgtt acaattatac aataatctgc agataattgc attagcaatt
    10321 ttcaaaattt gttgaaaaat ttggtattat gtcatttttt tatttatcca gcttaaaatg
    10381 tcctcattta ccttatcttt ttccagttca ttaagtattt catgcctgta atccttataa
    10441 agcttcattg taacatcggt cagttttgtt ttgtagacag catatagctt ttccacatcc
    10501 ttgccgaaat ttcccaaggg gtccttttct ccggataaga gtatcaaggg taaactcccg
    10561 ggtattttat ccaaatatcc tttatcgtat gaatacagca ttcctttaaa catataataa
    10621 aaagcattaa gtgtaaaaat aaaatttatt cttttgtcat ttatgtaagc atccaccact
    10681 tcttcatccc ttgtaagcca gtcagacctg gttctggaag gttcaaaata tgtattattc
    10741 ttttctaccg ccagcttgtt tataaatctg cttctgtacc tccatccttt gaaaactgcc
    10801 attagccttg aaattatcat tccgtacatt acctttgcat aaggcggata gccggttccc
    10861 ataattaatg ccccgtccat atcatggtat attgaaacat actgtcttac aagaaatgag
    10921 cccatactgt ggcccaatat aaaataaggc acttcatatt cttctttcaa gatatcctgt
    10981 aattttcgca tatctttaat aaccgccata tttccgtcac cttcactaaa aaaaccccga
    11041 tcttcttctt taagcacaga tgctccatgg ccaaggtggt catttcctgc aaccagtatt
    11101 ccattttcag ccataaatcg ggcaaaataa tcatatctgt caatatactc aaacattccg
    11161 tgagcaattt gcaatatagc tcttgtttca atttcaggta tccattttac cgcatggatt
    11221 tttgtttttt tatcagctga atcataataa aaatcttgtt ttattgccat aatcgcctcc
    11281 atttttttct catattttat gcaggctgaa gaattcagct gacagcctga tttattttta
    11341 aatattcttc tattattaag tcgcccatct tatccagagg atttttttta agaaaatcaa
    11401 caaatgtttt tgaaaagtta gatgtagcat tcacatcaat gatataagga ccgttactgc
    11461 ttacaattac gtcaatccca cccatcttaa tatttaatat cttcaatgta tttataacta
    11521 tattttctat atgcttgttt aatttataaa tattctcata aatggcaccc ctatgataag
    11581 aagatattcc agattcgtct gtacttcttt taactgctga aaaaacctct cccttaatta
    11641 cttcaattct aagagtaaaa ttgtccactg atttgatata ttcttggaaa atgaaatcaa
    11701 taggaggagt cgtttttaaa aattcaaata attcatctcc attttcaact tttatggtat
    11761 attttgatct tcctccgctg tttggtttta ctatgcatgg atatttaatc ttatccgggg
    11821 aaaaatctct ggtggtgcaa taaatttcag gggtgtttat tttgttttca ctaagagttt
    11881 tgcataccaa ttgtttgtca aaatcataca gcaaagcttc ataggaattt atcatgggaa
    11941 ctcctctttc atttaattcc ctaaaaaaat caatactttt ataatatgtt ttcaaatgtc
    12001 ccctgaaata tgagctggga aaaattctgt tgacaattaa acctgcagaa tcaaaaaaag
    12061 ccgcattcaa tacagcttcc tcgaaattaa taaatataac ctcaaaaccc ttattcatta
    12121 aatagtctct taaataaata tttgaccatt cctctgattc ataaattatt gcaatagaca
    12181 tatgcgtcct ccttattccg gggtatttct gcctaacaag gttaatttcc atttaagggc
    12241 tttaaaaagt ctgatatttt taaaagcagg tcatcaatat cacaagcctc aagtgacagc
    12301 ttttctatag gacacattcc cgaccggtca cgatttttaa taaatggaac cagtttatcg
    12361 tattcataat caatggaagt gtttttcaaa atatccaaag cattttcaga aataaggctt
    12421 gtacttattt ctttaatgcc tgcatgctta taaatcatcg ccgctgccct gcctacaacc
    12481 ctgtcggctg cacttgagcc ctgaagccga tcttttaact cattaagagc tgtataaaca
    12541 ggttttattc ccttgccctc acttgaaaaa attaccttcc catccttgac aattacaata
    12601 gccttttctt gagccttcaa taaatccttt gccagttcta tatccttcat catatgctcc
    12661 aatctgtatt atttattata aggtattatt ctgttatttg caataaaaat gccggttgat
    12721 gccggcatta tatttcttta cattttttct attttccaca aatttttcca tataatcaat
    12781 cgcccgtcaa cattatagaa agttgtactc agtaactatc ttttctctgt ctttagtaat
    12841 atcattaaaa tattcataaa acgacaacgc taaaaagctt cctgttatgt tatgcacatt
    12901 ttcatagccc agatttcgaa gcgccatgac agcattatag cttctttgac cggttctgca
    12961 atgtaaatat acgggtctat cctttggaat ttccgaaatt ctgtttctaa attcacttaa
    13021 gggaatattt aatgctccct taatatggcc ctcatcatat tcataccttt ctcttacgtc
    13081 tacaatatgt gcgccgcttt ccaccaatcc ccggacactg tcaacattta tctgcttaaa
    13141 gtctccgttc agcaagtttg aacctacata gcctgcataa tttacaacat cctttgcagt
    13201 ggaaaaagga ggtgcataac ataattccaa atcttttaaa tcatccaatg ttccgccgaa
    13261 tttaatcaca gttgctataa catcaaccct ttttacagca tcaccttttc cgattgcctg
    13321 agctccaagt attttaccgg taggaacttc atatattaac tttaaatgaa ggggctcagc
    13381 gccaggcatt atactcaccc tgtcatttaa tattattctt acaatatcat agttaatttt
    13441 aattttttga gacataatca tagattcgct taaaccggtt gctgctccat tgtagtcaaa
    13501 aacctttatt gccgatgaac ctatgaatcc cctgttaaag gtattcttat tgtttatatg
    13561 gtctgcagct gtcctggcag ccttctgggc aggtcctgcc aatgccagtt ttaccggtcg
    13621 atgagtcagt gcattgtata cttcaattgc atcacctaca gcataaatat ttttatcgct
    13681 ggttctgtaa tttttatcaa cctttattgc tcctgtttca cctatttcca gccctgcctc
    13741 ccgggcaaga ccaatttcag gttcaacccc tttcgccatt ataacagcat gagcatcaat
    13801 tattttgccc gacttaagat atgccttgtc attttcaaac ttttcaattt tttctccgat
    13861 taacaaatta atccccttgt caaaaacttc cttatgaaat atttggacca tgtcataatc
    13921 ataagtattt aatatttgcc tggaagcaat tattatagat acattatatc cggctaatcg
    13981 caaattttct gctgtttcta cacctataaa tccgccgccg attacaacaa tgtttttagc
    14041 tgtggattta ttgataaact tttttatgtt atcgatatca actacatttc tcacagtaaa
    14101 caaattggtt ttttcaagtc ccttaatatt ttcaattatt ggcgaagcac cgggagataa
    14161 aattaattta tcatagcttt ctgtgtaaac ttctcctgta agaatatttc ttacagatac
    14221 atttttgttt atcctgtcga ttgataatac ctcgttgttt attctggctt caatgttgta
    14281 ccggctcagg aagaggtccg ggtccattaa aattaaatct tccgcatcct ctataacgcc
    14341 gcttaaatga tagggcagcg aacagtttga aaaagaaaca tgaggtcctt tttcaaacat
    14401 aataatacgg tcatcttcac tgtttcttct gagccttgct gcagcagatg cacctcctgc
    14461 aactcctccc actattaata ttttgcgtcc catagcttct cctcttattt tctgtttttt
    14521 cctctgtatg aaccaattcc tccagatagg tttacaacat catatccgat ttttgccagg
    14581 taggaaacgg ttcttttgct tcttgcaccc gacaaacaaa acatataata ctttttaccc
    14641 atttgtaaat acttgtcatg attgttcaat aattggttca taggaatgtt ttttgcagtt
    14701 tttatgcttc ctgacttata ttcatattct tctcttatat cgaccaaatt tatttttccc
    14761 attaaatcat caacatcatt aatatttatt tcatttattt ttgtcttttg caataatcca
    14821 aacatattaa cctcctttta atgtattaat ggtatcagtt cttcgaaatt aatatgtaat
    14881 taaatcacaa aaccacaggt taccctgtgg tatgtgtttt tgtgctataa tgttattttt
    14941 tcaattcctc tcatataagg tatcaagatt tcaggaattg ttattgatcc gtcttcgttc
    15001 tgataatttt caagaatagc ggcaaatgtt cttcccacag caagtcctga accgttaagc
    15061 gtatggatat actctaatct gcccttttct tcaggcctga atttgatatt ggctcttctt
    15121 gcctgaaaat cttcaaagtt tgagcatgaa gaaatttcaa catatctgtt gtagcttggc
    15181 atccatacct ccaggtcgta ggtcttggca gagctgaaac ctatgtctcc ggtgcagagc
    15241 tctacaaccc tgtaaggaag acctaatccc tttaaaattt cttcagcaaa atttgtaagt
    15301 atctccagtt gagcatatga ctcttccggt tttgcgtaca taaccatttc aactttgtca
    15361 aactgatggt ttctgatcaa gcctcttgta tccctccctg cagaccctgc ttctgccctg
    15421 aagcatggtg tataggcagt caggtacatt ggaaggtcct tttcctccaa tacttcattt
    15481 ctgtaaatat tggtcacggg aacttcagct gtaggaatca aatagtaatc tcgttgactc
    15541 agcttaaaca tatcctcttc gaatttagga agctgtcctg ttcctatcat gctgtccctg
    15601 tttaccataa agggaggaga tatttccgta aagccgtggt ctaaggtgtg cttgtcaagc
    15661 atataggcag ttattgccct ttccagtctt gctccggccc ccttgaacat ggaaaaacgg
    15721 gttccggtaa tttttgttgc cctttcaaaa tcaaggatat ccaggtctac ccctaaatcc
    15781 cagtgggctt tcagttcaaa gtcaaacttt cttggctctc cccattttcg gatttccttg
    15841 ttgtctgcat ctgaagtacc cacagtgatg tcaggatttg gcgtgttagg aatattcaac
    15901 agctcttctt ctatcttttc atcgatttct tttacctgac cgtccaatgt ctttaccttt
    15961 tcggctaatt ccttcatttc ctgaagaatt ccggccacat cttttccttc ttttttaaga
    16021 agtggaacct gtttggatac tttgttctgt tctgccttca tgttctcaac ttcaacaagt
    16081 ttttcccttc ttgccttgtc taattcaaga atcttatcca ggctcagctc gggattcctt
    16141 cttttcaaaa gttcttctac ctcttttggg ttgtttctga ttcttttgat atctagcatg
    16201 tctaacctcc ataacaaact tgttatattt aattttaata ttatttcata ttatatccat
    16261 atacagggag atgcatcaaa tttgtatgct ccttatgtcg aacaaatacg gcatttcttg
    16321 cggttgacct gccaccattt ttcagaaaag tgactctgaa aactgggtta ttggcataca
    16381 aaaactcccg cctctgttta gagacgagag atactcgcgg tgccacccta attattgtat
    16441 ataaggagga catttgtcct ttaatgatat acaatcactt ggagaagtta acgccttcca
    16501 cgtctaagtt ttttaccctt agaaactccg gaacggattc aacaaccaat atgccgcctc
    16561 gcaccaaccg ccggctctct taaataaacc tgttgtttac tgctttccat cattgccata
    16621 tttggataat ataataacat tatatctttg tcaagcttta tttaatgtat tgaaggtgat
    16681 atgatacaca gcccttcagt cacatcatca tttttattat aaaacatatg attgttgttt
    16741 tcaggcacaa ttatactgtc tccctttgac aatgtgtaga ttttttctcc tacatggcat
    16801 tccatttccc cctgtataac atagatatat tccttaaagt catgtgttat accgtggtcg
    16861 cttgcccatg ttccgggatt cagtttaaac aggaaaatct caaatcccgt ttttttatca
    16921 ccacttttca atattggagt aatgaattcc aattcaacat taacatcgtt taatttcact
    16981 ttagttcttt cgttatgcgg tattgttata acatcatcag aggatttctc caaaaataat
    17041 actgatagag gcacctccaa tacatttgac aatcttttca aaaccgccag ggaaggagat
    17101 attaaatctc tctctatttg tgaaatatat cctgatgtta cgtcaataag ctgagacaat
    17161 tcaacaatag tcatattttt gctttttctg atatttctta ttttttcccc taccattata
    17221 acacccccat ctttataata ttattataca ccaaatataa tttttgtcat caaaaaaatt
    17281 gaataaatta atttttatac aacctttttt agtattattt aaaaaacaat aaatgaaata
    17341 tcacaaggtg gtaaaataat ataacagtcc aacttttatt ttataaagtc aaatcttatg
    17401 cttttcatgt aatacaaaat atgatataat aatccattga tactttataa ccatccaatc
    17461 atatggtcaa ggggatatga aatgtctgat aacagcaagt attcagaaga aattaaatat
    17521 ttagatgaaa ttattaatct gttaaaacag aaactggaat atgaaaaaag ccagcttgag
    17581 aatcaaaaat cagatttaat agagtcaaga agagagatgt gggaaaatac aacccattca
    17641 tctgccgact tcgacaagct gacagactta aaccagtatt taagcgctct gcaagctcaa
    17701 acctttactt atgcagagct tgcaaaacgt atttcaaaac atgaaaaaat gattgaaagc
    17761 ccttattttg caagaatcga cttcactgaa gaaggatatg atgatactga aaaaatatac
    17821 atagggcttt ttaatttaat ggatgataaa acccatgaaa taaaagttta tgactggcgt
    17881 gcaccaatat caagcatata ttacagaaat gaagttggac ctgttgagta caaagctcct
    17941 gcaggattaa taaagggtaa tgtgtcatta aaaaggcagt ttaaaatttc aaaaggcaaa
    18001 atggaatact ttttcgacag caatataaat attcttgatg aaatgctgat ggtggcactt
    18061 tcaaagaata tgacatcaaa aatgaaaacc attgttgaaa caatacaaaa acaacaggat
    18121 ttgattatca gagacctgta taatgattta ctggttgttc aaggggttgc aggcagcgga
    18181 aaatcatcgg ttgccctcca cagaattgca tatttgatgt atcaaggttt aaatttaaaa
    18241 ttaagcgcaa acaatattat tgttatatca cctaatccgc tgttttcaaa atacatatcc
    18301 aacgttcttc ccgaattggg agaagaaaat attaatgaat atacctttga aaatattttt
    18361 cataagctgt ttgaaaataa actttcaatg aaaacaaaaa gtgaaaattt tgaaaatatt
    18421 attaccgcag aatctgaaga ccaaagaaat tttttaagaa cctgtgatga atttaaagga
    18481 tcatttgttt tttcgaaaat catcgaccgg tttatagatt actatgaaca taagctcata
    18541 ccatttgaag atgtttattt taacggaaaa attattgaaa aacacgagct gtcaaaagca
    18601 tttttattga gtggcaagct tgatatgcca acaggcaaaa aattaaagat aattgaaagc
    18661 cgcatactgg aaaaggttcg ggaaaataga attgaaagac gaagtaaaat tgaaaaagct
    18721 gtaaacaagt taaataacca tgaatttgaa acagcttctt tcacgcgatt gcttgcagcc
    18781 agggaaaccc aggcgctggc agaaagagtg cgaaaattta cggaactgga cagttttgaa
    18841 gtctataaaa aactgttttc tgatattaat atttttaaaa aaatctccaa gaacctcgat
    18901 cttccggaaa gtattgatga aataataagc tttaccaata aagctttaag cgatccctac
    18961 aacatcccct atgctgatgg tacggcactc atgtacatga atataaagtt ggagggatgc
    19021 gacctgttca gcagtataaa gcaggttgtc gtagatgaag cccaggatta ttatcccatc
    19081 cattattata ttttaaaaaa tctatacaga tatgcaaggt ttacaatagt tggcgatgtc
    19141 aatcaatctg ttgaaaagga cagttctttt tcattatatg atgatattgt ttcaatattt
    19201 aattttgaaa agagcaataa agtgttcctg accaaaagtt acagaagttc ttatgaaata
    19261 gcaagattca gcgcaaaact gctgggagac gaaaacttca cagaattctt taaaagaaat
    19321 gaagaagagc cggcacttgt taaaataaac aacagcttac gcatgcagga tgagatagtt
    19381 aaaacgattg aaaaatataa atctgaaggt ttttcttcaa ttgccatgat atgcaaaaat
    19441 agaaaggaag catccgactt atattttaaa ctgagcagta aaaccaaagt aaagcttata
    19501 gactacctgg attatgacag tgttttggag ggtaccctta tactgcctgt ttatttggca
    19561 aaaggactgg aatttgacgc tgtgatagta tatgaagcaa atgataagca atataataca
    19621 aagtatgaca aaaaactctt atatattgca tgcaccagag cccttcacag gttatcagtt
    19681 ttttatacag gaaaaatgac aaggttttta gaggacctgc ggtgagctta tcttttgaga
    19741 atttccttac aacttctata agtggcggga gtatttcaca actacagaat ttgagaatct
    19801 ccggcattta aaaatccgct taaggtttag cggattttta ttattcaaaa catattgggt
    19861 ctggtcatat cgcaatctcc gcaaacaaat ttatttgttc catttggtca cataaatttg
    19921 gtgcttgttg cgtttgacct accatccata tgttcttcac tttggctcga acatatgggg
    19981 tctggtcata agcgggtgat gggaatcgaa cccacacagc cggcttggga agccggaact
    20041 ctaccactaa gtaacacccg caataaaatg gagataaggg gtctcgaacc cctggcctct
    20101 tgaatgccat tcaagcactc tcccaactga gctatatccc cgtattacat tttatatact
    20161 gtcttcaaat caaagaacat taatatattt aatttaatac atttttgatg atttgtaaag
    20221 aagtaaaata aaaaaagctc ctcattaaag ctactgagga gattcgaact cctgacctac
    20281 tgattacgaa tcagttgcgc tgccagctgc gccacagtag catatatata atttaatact
    20341 tttttctccg tttgtaaaca gtaaatacaa ataaaacagt aaagctttaa cctgattacc
    20401 tgggaaagag gcgtaacaca agttattgca aggcatatat tataatatat aagttatgta
    20461 taaaggagag tatattgacc atgtataatg ataatggaat gaataatact gtgtacagca
    20521 gcaacagata tgatatgtat gacagaagat ttgatccgga atatgatgtg atgtacgaca
    20581 gaagatttga tccggaatat gatatgatgt acgatagaag atacgataga agatatgaca
    20641 tggattttta tgacccgatt aggtattatc aattcccata ctgtgacagg tatggaagat
    20701 gcgaaaatcc tatttggtgg ctgttctggc ctttattttt cctctaaaat tatatttgac
    20761 agtaaacggg accttgaggc cccgtttgtt taaaacttac ggcttcccgg ctttaccggt
    20821 tttgaacccc tttcgcacag gggacagttt tcttcatcat aggtctcaaa gaataattct
    20881 actgaactgt atattggcaa tggaatttcg cttgcctttc tatccacaat acaaccgatt
    20941 cccactacta tgccgcctaa actttcaagt acctttgcag tttctcctga ggatttcccg
    21001 gtagttacaa catcctccat gattagtacc ctgtcgccgg gttttatttc aaaacctctt
    21061 ctaagagtca ttacattgtt ttctctctcg gtgaatattg cttttatacc taattgtctg
    21121 ccaagctcat aagctgctgt aatccctccc attgcaggtc caactataac attcaaatca
    21181 aggggggcaa ctttatccct aacaagagaa ataacctttt ctgctttatc aggatattgc
    21241 aggagctttg cacattgaca atatctgttg ctgtgtttgc cggaagaaag caggaagtgg
    21301 ccttcaagca aggcaccgca ttctttcaaa atttcaacta catcaatcat actatctcta
    21361 aacctcctgg attttcttat aattcattaa ttattgcgac tcaatgtttt attatttata
    21421 ttatatccat tattcatcaa tcaggaagaa aggtctcccg tgtttatata attcctctta
    21481 tttcatccaa ggattttaaa ttttcattgt caagaaattc ttccaggtca ttgatgatat
    21541 ctattgcaat atcaggtttt ataaagtttg ctgtccctat ttgcaccaat gaggcacctg
    21601 ccataatgaa ttcaactatg tcagctgcat tcattatgcc tcccaatcca caaatgggaa
    21661 ttttaaccat ttttgcagct tgatggacca tgcgaagagc aatcggcttt acgcagggtc
    21721 cggaaagtcc ggcataaata ttgtcaaata cagcttttct attttttata tccactgcca
    21781 tggcttgaac cgtgtttatt aaggaaattg catctgctcc ggcagcttca caggctgtgg
    21841 ccatgtccga aatattttct gcatttggcg ataacttgac catgatgggt tttttacata
    21901 tttttcggac ctttgacact atttcatagg cgaccgaaga tttcactcca aaagccatac
    21961 ctccgctctt gacatttggg caggaaatat taagctcaac tccatccacg tttgtatcat
    22021 ttagcgtata tataccttca aaataatcct gttcacagtg tcctcccagg ttggcaaaaa
    22081 taatagtatc gtacattttc attcttggaa gctcttcgct tatgaatttg tcaactcccg
    22141 gattttcaag accaatgctg ttcatcagcc ccatgggggt ttcccatagt cttattccct
    22201 cattgccgct ccttggagtt aatgtaagac ctttgctgca aattccccca agaatagaaa
    22261 tatcataaat ttgagaaaat tcttcaccga aaccaaaggt gccggaagct gccaatatag
    22321 ggtttttaaa cttaaaaccc attgcatttg tttctaaatt tgccataata ctcacctata
    22381 ataattcttc actgtcgaat acaggacctt ccctgcatgc ccttttgttt cctgtctttg
    22441 tttttacagt gcatcccaaa caggccccca taccgcatgc catccgcctt tccagtgaaa
    22501 gatagcattt gatgttttta tccctgcata tagctgcaac cctcttcatc attatttcag
    22561 gtccgcaggc tgcaacaaca tcgtattttt caaagggaac ataatccgtc acgaatcctt
    22621 tttcgccaaa gctgccatcc tctgttacaa taacacaggc tgcggcaaag gatttaaatt
    22681 catcagttat atacatatta tccaaatccc tgtaccctaa atatacatct gcattttttc
    22741 ctaaagtttt acacaaatac aagagtggtg caattccaat ccctccgcct attactgcag
    22801 tttttccttt cagtttatca ggctcaaatc cgttacccag gggaccgaat aactggagct
    22861 ggtcattttt tcgcaggctg cttattcttt tagtcccttg tccttcaatt ctgtatagga
    22921 atgaaacaag ttttccattt acgtcattaa tacttacagg tcttggaaga aggaatgaat
    22981 tgtcggttgt tttaagcata aaaaattgtc ccggcttcat ttcagattca aattctgccg
    23041 aaagtctgta tatattattg gaaatttgtt tgttttcata aacaggggca agtaaaacct
    23101 tcattgtttt ctccgttttt ttgcagttac ttcttgtcat aagccgtatc acagtataca
    23161 cacctgtata tttctttttc tctgtcgtta agcctgaatt tatgaactat ttcctgctca
    23221 actgaagtta tgcatcttgg atttttgcat ttaacgacat ttacgacttc ctgaggcagt
    23281 tccagattta ttttttctat tattactgaa ttatcaataa cattgattgt tatgttcgga
    23341 tcaataaagc caagcacttt aaggtccata tctatgttat tttctacctt tattatatcc
    23401 tttttcccgt attttttgct ttttgcattt ttaattatgg caacactgca gtccatcttg
    23461 tcaagattca aatagtgata tatttccata gccttgccag ccttgatatg gtcaattacc
    23521 aatccctttt ctacactatc aatattaagc attattccac ccccaataat ttcataatta
    23581 aagccatcct tatatacatt ccgcacttag tttgtgtaaa gtatgccgct ctgggatcat
    23641 cgtcaacttc cactgatatt tcattcaccc tgggaagggg atgcataaga atcatatctt
    23701 ttttggcatt tgccattttt ttattgtcca caatataact gtcttttaat cttatatagt
    23761 cagcttcatt gaaaaatctt tctttttgaa ctcttgtcat ataaagaaca tctaactggc
    23821 ctattacatt atcgagtctt tctacttctg taaactcgat attgtttttt attaaaattt
    23881 cttctcttat ataatccggt atttttaatt cttcaggcga tatcaaaaca aatttaacgt
    23941 tttcgtatct tgacaaagct tttatcaagg aatgaaccgt ccgtccgaat tttaaatctc
    24001 cgcagagacc tatagtaaaa tcatttaatt ttccttttaa tgtcttaata gtaagcaggt
    24061 ctgtaagtgt ttgggttgga tgctggtgtc caccgtcacc tgcgtttata acaggtatgt
    24121 ttgctttcat tgcagccacc atgggagcgc cttcttttgg atggcgcatt gctgctatat
    24181 ctgcataaca tgccacggtt cttattgtgt ctgcaacact ttctcccttt gctgcagagc
    24241 ttgattctgc tgacgaaaag cctgtaacac ttccccccag ccttaacatt gctgattcaa
    24301 agcttagcct tgttcttgta ctgggttcat aaaataatgt agcaagtact tttcccttgc
    24361 acacatctgc ataagaagca ggattttgaa ttatttcgtt tgctaaattc agtatttcat
    24421 ccagctcctc aagtgataaa tccatcggtt cgattatact tctgcctttt aacatgtgtt
    24481 tcctcctttt tagcctctcg ggactagctt aaagtgtttt cccttgttag ggcatacgca
    24541 gtctcattca ccaaaatatt tgctccctct ggtcgcatat tttgggaatt cgttgcggtt
    24601 gacctaccgc catttttcgg aaaatcatat gcggggacat tcctcttact ccaaaggcta
    24661 atccgatagt agaggtgtgt ccccattcct ctaataaatg gggttagggc atacaaaagt
    24721 cttccttgca cacagcaaag gaagacctga taaaagctct catatgtttc cttcccagcc
    24781 tcactgtgcc agattaaagg tttaacttca acttatacta tattattttt ctcttcatgt
    24841 caatatattt attgcaggtc agatggttca ttgtcaggtt gtttgtcagc ttgatatact
    24901 atttttgtta tccttctttc aacttcgctc cttggaagcc atacctgcct gtcacaggta
    24961 gtgcatttta ttctaaaatc tgcacctatt ctcagtattt cccaatcgta accgccgcag
    25021 ggatgctttt tctttaattt tacaacatcc cctacattta acttcatagg catggatgct
    25081 ctccttatat tgtaattgta ttggttgaac ttgaaatcat atccacaata tcatacatat
    25141 ttgaaataga gccaaccctt aatttgtcct taatttcaaa gaaatccaga catgttccac
    25201 atgaaacaat ttttacgccg ccctctttca gcttcatcag gtcatcaaca gactctgagc
    25261 cttcagtagt gagcttaact cctgaattca aaaatataag gaaatcggga tatggttttg
    25321 tctccgacaa agtgtacaga aaacttttca tgagtattct gcccagatca tccgacccct
    25381 tgcccaattt atcgcttgta ataaagtagc cggcattctt attttttatt tcggaaatta
    25441 tttctgtctg ctccttgttt tcaccgcttt tctttaaaac tatgtaagta ccgtcgtctt
    25501 tgtcttcggc tgttactgtg tattttaact gagttccgaa ctttgttaca ttaatttttg
    25561 ctgtttcatt gtcaacaatg actgtaattt cttcagctcc cccatccact tctttttttg
    25621 tcataataac cggctgaggg catgcttttt ttcttgcatc aactattttc attttttctc
    25681 cttagttttc ataaaaacct ttatatgata aataattcaa tataatattg ttgtcgtaga
    25741 atattttgct tgattcagat ttcaatactt cttgtgcaaa aggtttatcc tgcataaagc
    25801 ttaagacggg agctgctaat gcaaatatta aataaagaag tataacactt tttaatacac
    25861 ccataaaggc tccgaataat ctgtttgtaa gattcaagaa cggcagtttc attacagtat
    25921 ttataaaggc tgtcaaaata agcaatatta catatattgc aaggaatgta acaacaaagc
    25981 taatggatct tattatcaaa agactaatgt tgtccacaaa tacagcaaaa gtatcagaag
    26041 cttcgcttgc cattatatta tttacaaact tctgtagttc aacaggtatc ttgcttgtgt
    26101 tgctgactgc actcatggat agtttcgttt caaaataatc atgtagtttt acaaacagct
    26161 ttgtattgtt tagtaaaaag ttttctgcaa aattataaaa ttctttgctt aagaaaaacg
    26221 aaattacaag gcctaatgta tcaaagacag tcagtataaa ccctttccgg tagcccataa
    26281 aacacaagta tcctgcaaat actaatataa ttacatcaac tatcatttta tttccccctt
    26341 aatattattt tatttgtcct tcaataatct tgtttttaaa taaaatgtaa atattgtttc
    26401 cttcgatcat aaagtcttca aaactttcag tatcctcaaa taatttatca gtcttatttc
    26461 cgtgaagcag atatatggta ttattattat aagttaatgt tttattatca gctattttta
    26521 actttttgga tcctgcaggg gctgttttga tttccataac cttgccttca aaattatagg
    26581 ataatagttc agctgcctgg tccttttcaa acaatatatt aatcataccc tgagatattt
    26641 cataatcaag caccttgtta aatattttat tcttccacat taatttgccg ttatcattat
    26701 aaaaatatat gttttctgtt ccaataacta tcacattgtt attaacaacc tttgttttaa
    26761 ttaatatttc ctttgatatt attgaactcc ataattcaac atcttctata aaataatata
    26821 tgttgttgac aaaagctccg ttttcaaata caagagtaat aactgaatat gcgtcggatt
    26881 tatcactgat agatattcct gttattgtat cctggaattc cttgttgtct acaacaattt
    26941 cattgttatc atccattata taaagggttt gtccatttcc tttaacaatc attccggtct
    27001 tgttgttttc acgtgaaaca tttatgatat tcccttcaat ttcagctatt acaaactcct
    27061 gattgttttt gtccaaaact tttatattgt tatttgcctg tctgaatata taattgtctg
    27121 taacgaatac cctgttagaa aattccgtat tttgattttc ccataaaatt ttattatcga
    27181 aatctgtata gattgttttt tgattgttgt atgatattat acccctgtta aaaaattcaa
    27241 atccttcaag tgtacttatt tctgttgttt taataccttc aattttgtaa actttttttt
    27301 catccaaggt tttagttaat tccttgaaat aaggagagta aagcagggca actataaaaa
    27361 ttaaaattat tacaataaat aatattattt ttttcatata tcaccttgta aaattttgtt
    27421 aatcaattat accaaacttt atatattatt gcattaaaaa attattaaaa tttcagggac
    27481 attgaaaatg tccccttacc tgatagatat ctttttttgc gctgttttgt caacgggctg
    27541 ctatttcatt caatgctttc accgctgcat aaatatctgc ggttttatta aaaggaccaa
    27601 gactaaatct tacagcaccc agtttctctg ttccgattgc tttgtgggct aagggagcac
    27661 agtggagtcc cggccttacg gcaatattgt attccgaatc aagaatattt gcaacttctg
    27721 aagagtccat cccttctatg ttaacaggaa gaactccgga cctgtgctca atggactcgg
    27781 ggccgtataa tttgatttta gggttttttc tcagttctat aatgaatgtc tctaaaagat
    27841 ttttttcgtg gctgtaaatc gattttatgc ctctgtttaa aatatacctt actcctgcat
    27901 ttaatccggc aatacccggc aggttatgtg ttcctgcttc taatttatcc ggatagaaat
    27961 tcggatggtc aagactgctg gactggctgc cggtaccccc ttcttttaaa tgttttattt
    28021 ctatatcact gtttatcaaa agcgcacctg ttccctgggg tcccaatagt cccttatggc
    28081 cgggcactgc taaaaagctg atattgaatt tgttcatgtc aatatctaaa actcctgcac
    28141 tttgcgaagc atctaacaaa tatggtatat tatgtttctt acatatttta ccaatttctt
    28201 ctgccggaaa tatagtgcct gttaaatttg aaacatgagt tgtcacaatt aatcttgtat
    28261 tgttatttat tgcattttcc aaatccttta aatccatggt tccgtctgat gaacatttta
    28321 caattgtatt ttctacccct gatttttcta attccttaat aggtcttaaa acagagttgt
    28381 gctccatttc tgtggttatt acatggtctc cggaatttaa aacgcctttt attgcagtat
    28441 ttaaactgtc tgttgcatta aatgtgaaaa tcactttcat gggatcattt aaattgaata
    28501 gttcggctaa taactccctg gtttcataaa ttactctggc cccttcaatt gccattttat
    28561 gacttcccct gcccggattt gctccgtaga cagtcattgc tttcatgacg ttttcatata
    28621 ctgatgaagg ttttggaaat gttgtagctg cattatctaa atatatcata atatcacctg
    28681 ctgtttttta tatttcaatt cattatatgt aaattgccgc atttggatat tactaatctt
    28741 tatacacgat tcttcccttg catattgtat attttacctt gccccatagt tttcttccta
    28801 aaaacgggga atttcttgat tttgaaataa aatcaacatt ccagctttcg tcaatatcaa
    28861 atattacaag atcgcattta ttgccttctt tgattcctga ctctaaatta tacaatcttg
    28921 caggatttac ggtcattttt tccaggagct ggctgattgt aaggtgatta ttttttacca
    28981 aataggtaat accaactgac aaggctgttt ccagaccgac aatcccgctt ggagccttgc
    29041 ttaattccct atccttttct tccttgctgt ggggagcatg gtccgtagca attatatcaa
    29101 ttgtgtcgtc cttcaaacct gcgattattt tcattctgtc attttcggtt ctcaagggag
    29161 gattcatttt tgcatttgtt ctaaattcaa gtatatcatc ttcagtcatg gaaaagtgat
    29221 gaggagtggc ttcggcggta atatttgcac ccatcaattt agcccacctt attatatcaa
    29281 cagataagcc tgagcttata tgctgaaagt tgacttttgc gcctgttttt aaagcaatca
    29341 tgcaatctct tgcaaccatt acgtcttcgg caagttgagg tgccccttta atattaagct
    29401 ttttcgaaat tattccttca tttattccgg gactgtctat taaacgtggg tcttcctcat
    29461 gaaggcttat gggcaaattc agcctttttg cttccagcat tgcattcaat aatatatttg
    29521 tgtccattat aggaaatccg tcatcagaaa atcctgctgc accgttattt ttcaattctt
    29581 ccatatttac aatctcagtt cccttcaagc ctttggtcac ggctgctgct tgaagaacat
    29641 ttattgaaga atgttttatt atattattta tatatttgag agtttctata ttatccactg
    29701 caggtgctgt gtttgccatg catacaactg ttgtaaatcc gcctcttgca gctgcttttg
    29761 aaccggaaat aatatcttcc ttatattcaa atcccggctc tctgaaatga acatgtatat
    29821 ctattaaacc gggtgctata atcctgcctt cagcatttat taccctttcg ccatcaactt
    29881 ttatattttt atcaatcctt ttaataatgt cattttctat caatatatct gcaatttcgt
    29941 ctattttgga agccggatct attattcttc cgttttttac tatcatactg tacctgcttt
    30001 cttctttact cagtatccat tttattatag gtaatcgtct tgcttcacac cgatacaatg
    30061 aatacattgg ggcaagggcc caagcctttg cttcattgat tgtattcatt atatcataag
    30121 tgcaagccgt aaaatcggct tgcttcacac cgatacaatg aatacattgg ggcaagagcc
    30181 caggcctttg cttcattgat tgtattcatt ataacacaac acctttgtaa aaagaatctt
    30241 tgcaaaataa aatattctct gctttacaca gagaatattt tgaattttat tttattttaa
    30301 tttttccatt agcttgtcat attcttcctg ctgtgcaatt tcataagaat tacgcttata
    30361 gtaatgagtg tgaagatatt tgtgtgaaat gtggccgttg ggctctttca agaattcttc
    30421 atacagcttt attacctcag gatttttatg tgactttctc aagggcagag atttgtcaag
    30481 ttcgtacaat gcatctgcac gtttagcctt tggattgaca tcaagcctct tctttgcaga
    30541 tacgtgagac tgtccccctc cgtgcacaca tcctcccgga catgccataa cttctatgaa
    30601 atgatactcc ttctcgccct tgttcacagc atctaatact ttcgccgcat tggctgttcc
    30661 gtgggcaact gcaattttta tttctgtatc cttgccgtca attggaagca ctacggatgc
    30721 ctctttaaca ccatcaaggc ctcttacgcc ttgatactcg atatcttcca aatccttccc
    30781 tgtcagtatg tctgcaactg tacgcagtgc tgcttccata actccaccgg tagtaccgaa
    30841 gattgcagcg gctcccgtat attgtcccaa tatgctgtca gcctcttcat caggcaggtc
    30901 tacaaaatta attcttgcct gtttaatcat atctcctaat tctcttgtag tcaatgacac
    30961 atccacatct ctcaatccgt ctacttccat ttcaggtctg cttgcttctg ttttctttga
    31021 tgtacatggc attattgata cagaaaatat ctttttcggg tctattcctg ctttttgagc
    31081 atagtatgat tttaccattg caccaaacat ctgctgtggc gacttgcatg ttgataaatt
    31141 atccaggaat tcaggataat ttaattcaca gaaacgaatc catcccggtg agcaagaggt
    31201 tatcataggc agcttgccgc cgtttttaac tctgtctaaa agctctgtac cttcttccag
    31261 aattgtaagg tctgctgaga aatttgtgtc aaatactttg tcaaaaccga ttcttttcag
    31321 tgcagcaacc attcttccgg tcacatctgt accaaccggc agcccgaatt cttctcccaa
    31381 agaagctctg actccaggtg ctgtctgcac cacaactatt ttttcaggat cgtctatggc
    31441 atcccatacg tcatctacat aggttctttc tctcaaagct gccaccggac aggcctgtat
    31501 gcattgtccg caatatacgc agggtacgtc cttcatgctg taattaaatg cgggagcaac
    31561 ctccgtaaca aatcctcttt ttgtaaaatc caaaattcct attccctgta cttctttgca
    31621 ggtgctgacg catcttccgc aaagaatgca cttgcttgta tctcttacaa tggaataaga
    31681 taaattatca atttttgctt ctctcttttc accttcatat tttatgtcgc tgatattcat
    31741 ttcatcacat agtttttgaa gctcacaggt ttggtttctt agacaagtaa ggcattctct
    31801 gttgtgattt gcaagtatca gctccagatt cattctgacg gcatttctaa ctctttttgt
    31861 attggttttt acattcattc cttcttgaac ttgtagcacg caggtggcag gcaggtttct
    31921 catgggaacg ccttttatat caacttccgc aatgcacagt ctgcaagctg ctatttcatt
    31981 tatatccttt aaatagcaga gtgtaggtat atctatacct gcttccttgg ctgccaggag
    32041 caccgtatag tcttttggca cgctgacatt tattccgttt atagttaaat ttactttatc
    32101 cataatacac ctctttctac ttcctgataa ttgcatggaa cgtacaagca tccatacaag
    32161 caccgcactt aatacacttg tcctgattaa ttacatgaac ttccttcact ttgccttcaa
    32221 ttgctcctgc cgggcataat ctcgcacact tagtgcatcc tttacagtcc atggttatca
    32281 tatatttcaa aagtgactgg cagacgcctg ccgggcagcg tttttcctta acatgggctt
    32341 catattcatc cctgaaatac ttcaatgttg ataaaacagg atttggcgct gtctgtccca
    32401 agccgcatag tgaagtttcc ttgatattta tagccagttt ttccagctta tcaagatcct
    32461 ccatggtgcc ttttccttca gctattttgt ttaacatttc aagcattctt tttgtgcctt
    32521 ccctgcaggg tgtacatttg ccgcaggatt catcaacggt aaagtcaagg aaaaatcttg
    32581 caatatcaac catacagtta tcttcatcca ttacaatca
//
LOCUS       DAOPUT010000013        28623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_1603_length_28623_cov_18.853157, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000013 DAOPUT010000000
VERSION     DAOPUT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 28623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28623
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1603_length_28623_cov_18.853157"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..2428)
                     /locus_tag="PKK75_02570"
     CDS             complement(38..2428)
                     /locus_tag="PKK75_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNZ82509.1"
                     /translation="MKGITMIQKSKSVLEFDKIIERVAGFAETNKGKEEVYKIDTSDS
                     LEGVSFKQKQTAQALSIIIEKGSPPLGGISDIKEYVKRGAVGGIISPRGLINCADTLR
                     AARLLKNYVLLNNDDRTYDLLEALCEGIYTDKSIEERIYSVIISEEEIADDASSELKR
                     IRREIQIKNNSIKNKINSIVSSSSMQKYLQETIVTMRNDRYVIPVRKEYRSMVRGIIH
                     DQSATGSTLFIEPMAVVEMTNDISNLKIEEKKEIDRILLELSLMIGDISSEMLNNQEI
                     LTELDFIFAKGKYAISINGIEPKLNDRGYIRIKKGRHPLIDRETVVPIDAVLGDEYTT
                     LIITGPNTGGKTVSLKTLGLFTLMGMAGLHLPAEYGTETSVFSAVYADIGDEQSIEQS
                     LSTFSSHMTNIVKIMKEVDEKAFVLFDELGAGTDPTEGAALAIAILDTLHERRILTAA
                     TTHYSELKLYGLTTEGVINGSVEFNVETLSPTYRLLIGVPGKSNAFEISRKLGLQQNI
                     IERAKQNIETDKIEFEDALKQIEENRIYIEEKKQEINRLESESKKKHSDFLTKERKSL
                     EKSEKLVDDANYEARKIVENAKREVSEIIKELRKLNLEMDKDKNRRVNELRQTLSDMT
                     REFSGNIFTDQIEEEDTYDKDTPLKTGDAVMVRTINQKGFIISDVDDSETVTVQMGLI
                     KTKVKKADLVKIKSDEEVKQKTGTSGMVKLKTSSISPVIDLRGYNLDEALMELDKYLD
                     DAFMSNLNEVQVIHGKGTGVLREGVSQFLRKHKHIKESRLGTFNEGGDGVTIVKLK"
     gene            complement(2584..4755)
                     /locus_tag="PKK75_02575"
     CDS             complement(2584..4755)
                     /locus_tag="PKK75_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HNZ82510.1"
                     /translation="MKAELLAPAGSIESFYAAVNSGADSVYLGGKFFNARQSSQNFDN
                     EEMKHIVKYAHNKGVKVYVTLNTVLKDNEIIDALNYAVFLYENDVDALIVQDLGLLFL
                     INKYLPDFPVNISTQAAVYDEYGVKFFEQVNVKKVIMARELSIEELKETAKNTNTDLE
                     VFIHGALCTCYSGQCYMSSFLGGRSGNRGKCAQPCRLSYSFYDKEADREIEDYNTVPL
                     LSLKDFIAGESIYELIEAGIKTFKIEGRMKGPAYTSSVVEYYRQIIDNYYSGNNTDTA
                     DLRDKAIQTFSRGYTNGYLKPSKDDEMYAKLSSGIKGENIEEIEEDVKDKAQEFSRFR
                     RNKISFSIELKIGEQAVLKLFDGENTVEVKSEEACESSLRNFVTDEIINDQLSKLGNT
                     IYELDGLEIKKDEGVFIRKSTLNQLRRDAVEILYDKCGRVYDRREIKAFSYNDIFSFN
                     NKKAQPPKLSLKINSIDELKYADNIKVKRLYVPYYLNTEDVKNIKIEEKYLWIPNIVS
                     MADYKFLEKNLRLYEEIFDGVCVNNVGSFYFFRKNSKLKLHCGPFFNIINTFSEELLR
                     EMGVDSFTFSVETNIRDMESMMKHSSLSSEIIAHEYVQLMAMKNCPMSMIKNCKNLQD
                     CEKCSYRNKYMLKDRKGVYFNIERQNKLTYIYNSVPLTIIGKAHDFVGTNIEFFFIDT
                     KNIEDAEEIIDALYCEINGVKIPDVLQENKFTRGHYLKNIL"
     gene            complement(4914..5996)
                     /gene="zapA"
                     /locus_tag="PKK75_02580"
     CDS             complement(4914..5996)
                     /gene="zapA"
                     /locus_tag="PKK75_02580"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNZ82511.1"
                     /translation="MNRVEIVIADKKYNVKTDESPDYVKKIETVLNEQINLIANQNKR
                     FNDMDNLVLSAFVIVDSYLKLNGEFVEYKKEICEEIQTLKEEKESFEREKEESVSKAA
                     DAIIEKEKFKEKLLARDNDREYLNSQISKLQERVSEQDQQLLKSEVLINELKIKNEEL
                     LEDIDELSKERENFTKEISFMNNTKASLNGRISKLQLKLNEKDQELIKLEKDIRELKS
                     SVDDKSQMLYNFSDEQQKMNLLVDSKEKDIESLINKINLLQNKLNEKDETIAAKDKLI
                     NDLKNNEGSVKEIYESINDEKEKYLEELLMLNSDKESLINSVNLLQEKLNRKEAENFQ
                     NQLEINQLKKDNAALMELLDEESSKS"
     gene            complement(6134..6847)
                     /locus_tag="PKK75_02585"
     CDS             complement(6134..6847)
                     /locus_tag="PKK75_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNZ82512.1"
                     /translation="MKPIIGVTVGRSTNDGYSYETINESNLKALILSGAIPLMLPITV
                     EDELLDEYLKIVDGLCFSGGEDICPLIYGEDPIKEVKEIDHIRDEFEVRLFQKAAAKN
                     IPVLGICRGSQIINVSYGGSLYQDINVQKDGTSGHMPNYNGAAYPHHKVQIKKDTLLY
                     DILQTEEIPVNSYHHQAVKNVAEGFNVAAVSKDGTIEAIESVNHSFIIGVQWHPEILF
                     ERYPVFLKIFKALVKASSK"
     gene            complement(6844..8694)
                     /gene="dxs"
                     /locus_tag="PKK75_02590"
     CDS             complement(6844..8694)
                     /gene="dxs"
                     /locus_tag="PKK75_02590"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNZ82513.1"
                     /translation="MKLLENINTPDDIKHLNVDELNRLCSELRQYILEVVSKTGGHLA
                     SNLGVIELTVALHKVFNTPRDKIIWDVGHQTYAHKILTGRKNDMSTLRQHDGISGFPK
                     RKESIYDVFDTGHSSTSVSAAMGMARARDLKKEDYEIIAVIGDGALTGGMAFEALNDA
                     GRSNTKLLIILNDNEMSISQNVGGLSRYLSRIRTTRGYLTTKRDVEKLLNKIPVGRQI
                     KSVLKRAKDGIKQIVVPGMLFEELGLTYMGPVDGHNMHELIEYLETAKNIEGPLLMHV
                     ITKKGKGYRFAEERPNEFHGISAFNLETGQPLNNSALSYSDYFGIKMCEIAEKNEKVA
                     AITAAMTDGTGLQDFALKFPNRIFDAGIAEQHAVTMAAGLAASGIVPVVALYSSFLQR
                     AYDQVIHDVALQKLHVVFAVDRAGIVGNDGETHQGVFDEGFLSSIPNMTVLSPADCRE
                     FGNMLEFAVFFDGPVAIRYPRAAMNENIKGSGSDLVLGKGLVVEEGKDITIAAAGKMV
                     KTAIETREILKGENIDAEVLSLRFIKPIDKELLLRSVNKTKYLVIIDEALTFASYGAN
                     IISFLPKEANVLFKTLPDEFIKQGSIDELLIENKLDADSIAADIINWRKK"
     gene            8947..>9278
                     /locus_tag="PKK75_02595"
     CDS             8947..>9278
                     /locus_tag="PKK75_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNZ82514.1"
                     /translation="MEKKWVYLFKEGSASQKGLLGGKGSNLAEMTNIGMPVPQGFTVT
                     TEACTEFYNQGEKLSDEIISQIKSYLAILEEQTEKVFGDVENPLLVSVRSGAKFSMPG
                     MMDTILNLG"
     assembly_gap    9279..9378
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9379..11343
                     /gene="ppdK"
                     /locus_tag="PKK75_02600"
     CDS             <9379..11343
                     /gene="ppdK"
                     /locus_tag="PKK75_02600"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNZ82515.1"
                     /translation="DIPHDIGTAVNVQSMVYGNMGDTSGTGVAFTRNPSTGENKVFGE
                     FLINAQGEDVVAGIRTPLTIDKLKEIMPEIYDEFISTAEKLEQHYKDMQDIEFTIEEG
                     KLYMLQTRSGKRTADAALRAAVEMVDEGLISEEEAVLRVDPKSLDQLLHPKFDPDELK
                     KTAPIAKGLPASPGAASGKIYFTADDAVEASLKGEDTILVRKETSPEDIEGMSKAKGI
                     LTARGGMTSHAAVVARGMGKCCVAGCESAVVDEKNKTMTVNKVVYNEGDYISLDGSTG
                     SVYVGVIKTVPANISGNFEKLMVWADEFRRLKVRTNADTPHDSETAVKFGAEGIGLCR
                     TEHMFFEESRIFSVRKMIISDTLEQREKALAEIMPMQKNDFKGIFKAMGVRPVTIRLL
                     DPPLHEFLPVEEEDIVELAEAMDVPLKELKAKISSLHELNPMLGHRGCRLAISYPEIA
                     KMQARAIIEAAIEVSREENIEIVPEIMVPLVGKKEELVILRKLIIETAEETKKEFKSD
                     LNYLVGTMIEIPRACLTADEVAEYADFFSFGTNDLTQMTFGYSRDDAGKFLEDYKEAR
                     ILEQDPFETIDQEGVGKLVAMAVTLGRKAKEELHLGVCGEHGGDPASVEFFHRVGLDY
                     VSCSPFRVPVARLAAAQAAIKDKLNVHIDK"
     gene            complement(11453..11989)
                     /locus_tag="PKK75_02605"
     CDS             complement(11453..11989)
                     /locus_tag="PKK75_02605"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNZ82516.1"
                     /translation="MKLIVAVARDWGIGNKGELLFNVPQDMDFFKKTTLNKVVVMGRK
                     TLLSLPGGKPLINRTNIVLTRDVNFEKEGCIVVNSTEALFEELKNYNSDDVFLIGGGK
                     LYNELYPFCSEAYITKFDAVLEADTYLHNFEEDKDWLLTYASELHEHQGLRFIFTTYK
                     NLAYEKGTGGLPINVNSF"
     gene            complement(12000..12830)
                     /gene="thyA"
                     /locus_tag="PKK75_02610"
     CDS             complement(12000..12830)
                     /gene="thyA"
                     /locus_tag="PKK75_02610"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNZ82517.1"
                     /translation="MSTADKIFIQMCKDILTNGVWDTAYDVRPRWEDGTPAHTIKKFG
                     VVNRYDLQESFPILTLRKTNFKAAVDELLWIWQKKSNNVNDLNSRIWDSWADETGSIG
                     KAYGYQLGIKHKYREGDFDQVDRVIYDLKNNPASRRIMTNIYNFQDLHEMHLYPCAYS
                     MTFNVSGNRLNAILNQRSQDVLAANNWNVVQYSILVHMMAQVSGLVAGELVHVIADAH
                     IYDRHVPIIEELIEMESHDAPVLEIDKSINDFYDFSVDSFNLVDYKYNPFKVKVPIAV
                     "
     gene            complement(12942..13136)
                     /locus_tag="PKK75_02615"
     CDS             complement(12942..13136)
                     /locus_tag="PKK75_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82518.1"
                     /translation="MNDFEKIQKMYDNGFRCIRYDDTKDGDMCIYFKNFDNEDSEALR
                     VSDFEQKMQIKNFIRQNTMR"
     gene            complement(13234..13998)
                     /locus_tag="PKK75_02620"
     CDS             complement(13234..13998)
                     /locus_tag="PKK75_02620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ82519.1"
                     /translation="MVKGILFDKDGTLIDFSLWRNAGIKTIQTILNEYDINGEKLNKD
                     LQRAIGIKENGVEPFGALAYSSHENVAYELHSILKKHVNIELDRFQTHVVELLRKEVL
                     KDDADFKEIVSIKKLYEYLNSKNIKMGMATADSSQSVMHMINTLNLSDSFDFIGSYDG
                     TMKRKPHKDMCMRFCSMYGLEPGQVAIVGDSYNDMLFALNSGAIGVGVLSGVSSKINL
                     KDVANVIVASAGTLFDEYVLEALDEKYYGARELRTA"
     assembly_gap    14094..14193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14526..15800)
                     /gene="obgE"
                     /locus_tag="PKK75_02625"
     CDS             complement(14526..15800)
                     /gene="obgE"
                     /locus_tag="PKK75_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNZ82520.1"
                     /translation="MFVDMAKISIKAGKGGNGSVAFRREKYIPMGGPAGGDGGDGGSV
                     IIVADEGLRTLMDFRYKNHYQAENGEDGRGKKQYGADGKDLYLRVPPGTLIKDKETGI
                     VLADLKTGGQEYVAARGGHGGKGNVKFKSSIRRTPRFAEPGGKGDEREIVLELKLIAD
                     VGLIGFPNVGKSTILASVTSAKPKIANYPFTTLKPNLGVVSIGEGHSYVLADIPGLIE
                     GASEGAGLGLDFLRHVERTRLLLHVLDASGQEGRDPVEDFYKINEELSQYSEKLSQKH
                     QVIVLNKMDLPEAANNVEKINKIFSDKYEIIQVSAATGQGLDFLKKRAYERLIQIEEE
                     IEFVDENAVESFFDKKDRDTIVVSKEDDYFSAEGDFLERLVASTNFDDFESFSNFQKV
                     LVDKGVIQKLRDLGAVEGDLISVCGVEFDFLD"
     gene            complement(15947..16240)
                     /gene="rpmA"
                     /locus_tag="PKK75_02630"
     CDS             complement(15947..16240)
                     /gene="rpmA"
                     /locus_tag="PKK75_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNZ82521.1"
                     /translation="MLLKLNLQLFAHKKGVGSSRNGRDSNSKRLGVKRTDGQFVLAGN
                     ILVRQRGTKLHPGINVGIGSDDTLFAMVDGKVKYERKDKTRKQVSVYPVELEA"
     gene            complement(16243..16569)
                     /locus_tag="PKK75_02635"
     CDS             complement(16243..16569)
                     /locus_tag="PKK75_02635"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HNZ82522.1"
                     /translation="MITVTIFKTRDEIKGFMVKGHSDYAEAGFDIICASVSILSYTAL
                     NSLNLVAGIEPQNIEYAVDDEGMMSLKTLKNNDKTDIVYRNLIVGMELLLEDYSDYIT
                     LKFEEV"
     gene            complement(16571..16882)
                     /gene="rplU"
                     /locus_tag="PKK75_02640"
     CDS             complement(16571..16882)
                     /gene="rplU"
                     /locus_tag="PKK75_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNZ82523.1"
                     /translation="MYAIIETGGKQYRVQEGDVVRVEKLSIADGETVKFDKVLLVADE
                     GKLNVGKPYVDGAVVEGVVENQGKEKKILIFKYKSKKDYRKKQGHRQPYTQVKIEKIV
                     G"
     gene            complement(17073..18569)
                     /locus_tag="PKK75_02645"
     CDS             complement(17073..18569)
                     /locus_tag="PKK75_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNZ82524.1"
                     /translation="MKQVLVSSSVFFNHAAIIQDGRLVDYHFELNNKQNVSGNIYKGK
                     VMNILPGMEAAFVDIGLGKNAYLFFDDLLSDKFLQELRIKRKDAKNINKVLKTGDELL
                     VQIVREPIGEKNISVTTDISFTGKYIAFIPNTMGVNLSKKIKDRDERKRLEEIGKSIM
                     ADGNGMIIRTFAKGCPREAIEKEYEMLSSIYMQIEREYKYSYAPKLLYKSNSLNERLF
                     LDYIDSTVEEIYVEDKITKDRFKDMISGLSGNDLKNIKVILEQRVFEKFNIENQVKKL
                     LDRKVELKNGGSIIIDMTEALTVIDVNSGKYTGSRNMEETALAIDLEAVEEIARQVKL
                     RNISGIILIDFIDIKKKESIALLKAKAKSLFKDDKTRTNVIGITKLCIMEITRKKNKE
                     NFYNLMTEECVHCSGSGRSMSKAFIFMEIEEIIKNIKLNTSSEAVVLNVGCLLYNKIV
                     KSCMDIIAEIEKKYDIKIYIEQDEHILSDEIIAGKMGKLSNIDGIYRI"
     gene            complement(18583..19260)
                     /locus_tag="PKK75_02650"
     CDS             complement(18583..19260)
                     /locus_tag="PKK75_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNZ82525.1"
                     /translation="MCKIICKYTKTGYLKYISHLDVLRFIQRSVKRAGINAKYSEGFN
                     PHMKTSFGYPLSLGIESTGEYFELELDESIDPNLFTEKMNMVMPKEMQVIKAGYTQNT
                     DSLMKRCAYAQYSINIESDIDIDKLNKYLDEMIKIGEIYYRDKKNKKNKSVTRELNTK
                     DLLHYLKAENINNKTKIAAVFKTQESGSMKVEELIKLLRKKGFETDYFTVMKIDALDK
                     DMKPILQ"
     gene            complement(19248..21098)
                     /locus_tag="PKK75_02655"
     CDS             complement(19248..21098)
                     /locus_tag="PKK75_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNZ82526.1"
                     /translation="MIDKRKLDRELLSAEKPARYVGSELNSVIKNKDIDIRFAFAFPD
                     IYEVGMSHTGLHILYHLLNSMENVWCERVFAPWTDMEEVLRKNKIPLYGLESKDELTA
                     FDFIGFTLQYEMSYTNVLNMLDLGNIPIRSKDRDSRMPLVIAGGPCAYNPEPLHEIID
                     LFVIGEAEEVLPELINLYSDEKKKGYEKNSFLEKAAALEGVYVPKFYEEIYNDDGTIK
                     ERRLLNNNAKEIIRKRIIRDLDKSYYPEKSILPYIDVVHDRVVLELFRGCTRGCRFCQ
                     AGMVYRPVREKRADTLLEQADSLLRSTGHDEVSLTSLSSGDYSCLPDLTLKLLDTYEN
                     QNVSISLPSLRLDSMTFDIIERLQEVRKSGLTFAPEAGTQRMRDVINKNLTEDQILGP
                     VETAFNLGWSTVKLYFMIGLPGETMEDVLGIKDLSYKVKDKFFHRPIEDIKGNLKINV
                     SAACFVPKPFTPFQWFPQNNMDQFYDKAKALQREIKDKKISFSYHEPRLSYLEAVFAR
                     GDRRLSRALIKAWESGCRFDGWYDMFDFGKWMDAFEETNTDPDFFALRQRELTEVLPW
                     DFIDIGVTKKHLISEYKNALDCATTPDCRIECSSCGVNYNLLGGACPCAK"
     gene            21789..23108
                     /locus_tag="PKK75_02660"
     CDS             21789..23108
                     /locus_tag="PKK75_02660"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNZ82527.1"
                     /translation="MNYEEAIEFIHSTYKFGSKLGLERIKRLMEMLGNPHNSYEIIHV
                     AGTNGKGSTCSLIHEVLIESGYKTGLFISPYLEEFTERIQINKSHIGKNSLARITALV
                     KEKVDVMISEGYDHPTEFEVVTAIGFKYFQEENIDFLVLEVGLGGRFDATNVIEKPLV
                     SIITSISYDHMEQLGDTLEKIAFEKAGIIKEDSSVVIYPQAENIKNVIKEKAEEKHSL
                     VYEPDINNIEKISSDITGQWFKYLKSDVFDLPVIKINLLGSHQLLNALTALSALELIK
                     KQGCSISSESIINGFSNCRFPGRFEIINKDPLIILDGGHNIDGINSFTKTLREYFKDK
                     ATFFFGILKDKKPVEALELLLPYAKEIYTLTPLSPRALKAAELAELIKKHTKIKVIPL
                     DNYEDILPLLGNAGKSDTIAFSGSLYMIGSVRTLLRNNGFANNIKNL"
     gene            complement(23095..23445)
                     /locus_tag="PKK75_02665"
     CDS             complement(23095..23445)
                     /locus_tag="PKK75_02665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNZ82528.1"
                     /translation="MIVKRGDVYYADLSPVIGSEQGGVRPVLIVQNDIGNRYSPTVIV
                     SAITSQINKAKLPTHIEISSEDFSLPKDSVVLLEQIRTIDKKRLKEKIGRFDKNLMGE
                     VDDCLKISLGLIDF"
     gene            complement(23448..23723)
                     /locus_tag="PKK75_02670"
     CDS             complement(23448..23723)
                     /locus_tag="PKK75_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNZ82529.1"
                     /translation="MADNKKVLISIPENLLRELDNAVKEEGTNRSDFIRKSIRFYLIE
                     KRKLEIRNKMKAGYLEMSKINKSITEEYSEGDYEDFLSYETKLLGSE"
     gene            complement(23823..24320)
                     /locus_tag="PKK75_02675"
     CDS             complement(23823..24320)
                     /locus_tag="PKK75_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82530.1"
                     /translation="MRLIALCLVLMIAASGCAAEDNPFILPVNYSAEALMKVYTDKTE
                     NDYKVNIILRDGNYSIRTESEDESWNYAYVSGNRLILNNDKFPESSVTLENYKLNKTL
                     LHDFDFRKFDILENIPEELIYWDGEYKHVLNFSKENLLPKTIYIYNNDKLVKAIQYET
                     IKIEE"
     gene            complement(24482..24847)
                     /gene="acpS"
                     /locus_tag="PKK75_02680"
     CDS             complement(24482..24847)
                     /gene="acpS"
                     /locus_tag="PKK75_02680"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNZ82531.1"
                     /translation="MIIGTGIDITEVERIKKNMENTEFLKKIYSDKELKYLKTRKFNP
                     QTAAGMFAAKEAVSKCLGTGFSNFGPLHIEILKDDKGKPYAVLSGNALKRAEELNIKN
                     IHISISHTSEFALAQCIAE"
     gene            25043..25672
                     /gene="upp"
                     /locus_tag="PKK75_02685"
     CDS             25043..25672
                     /gene="upp"
                     /locus_tag="PKK75_02685"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNZ82532.1"
                     /translation="MNNVTVVYHPLIQHKLTMLRDKNTPPKDFRELVREISMLMAYEV
                     TRSLPLKDIDIETPMAKTTGKTLSGEDIAIVPVLRAGLGMVDGMLDLIPNAKIGHIGL
                     YRNEETLEPVEYYCKLPADIERRIVIITEPMLSTGGSMIGALDMLKKRGAKKIKSIHL
                     VCAPEGLKRVTEAHPDVEIYTAAIDEKYDETGYIVPGLGDAGDRLFGTK"
     assembly_gap    25800..25899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25900..26838
                     /locus_tag="PKK75_02690"
     CDS             <25900..26838
                     /locus_tag="PKK75_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNZ82533.1"
                     /translation="MRHIFIINPIAGNGKLQEKLEESIHLELKDKEIDYEVYTTKSKE
                     DTRNFAENKCRENLPSVLYSCGGDGTLHEIINAAFGYKHVSIGIIPVGSGNDFIKNFT
                     NKANFNDITLQTEGKSLELDLIKANDEYTATVLNIGLDADVAFNMNKFKKIPFIQGPT
                     RYYLSILYSLFSKLGKQIQVKTDNDFINGTFIIGVVANGQYYGGGYKCAPKAQLNDGI
                     LDLCFVENISRLKILSLLGSYKKGEHLENPKISKYVTYDKISRAEINFHGPTNVCMDG
                     DKYIYSEINISTEKNALNFWLPKGVEIISAEYVKSN"
     gene            complement(26977..27486)
                     /locus_tag="PKK75_02695"
     CDS             complement(26977..27486)
                     /locus_tag="PKK75_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HNZ82534.1"
                     /translation="MKKNNQFITRTALIAAAYAALTYAFAGLSYEQIQFRISEILVLF
                     AFIEPGYGLGLVLGCILANLASPLGLIDILAGSFATYLALVFIVKVRRLFGLNKKALI
                     LASLGPVISNALLVGAELTYLFNTPFLLNAFYVGAGEFTVVSLAGTVVVNSIMKNSVL
                     TEKLSFQKF"
     gene            complement(27628..28020)
                     /gene="rpsI"
                     /locus_tag="PKK75_02700"
     CDS             complement(27628..28020)
                     /gene="rpsI"
                     /locus_tag="PKK75_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNZ82535.1"
                     /translation="MMDAQYRGTGRRKKAIARVRLVPGTGKININERDLEDYIKYETL
                     RNVVREPLMITDTMGQYDVLVNVKGGGYTGQAGAIRHGISRALLQVDNELRPLLKKAG
                     FLTRDSRMKERKKYGLKKARRASQFSKR"
     gene            complement(28036..28464)
                     /gene="rplM"
                     /locus_tag="PKK75_02705"
     CDS             complement(28036..28464)
                     /gene="rplM"
                     /locus_tag="PKK75_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNZ82536.1"
                     /translation="MKSFLAKPNEVERAWYVIDAEGKTLGRLATEVAKLLRGKHKAIY
                     TPHVDTGDYVIVVNAEKVVLTGKKLDQKLYRHHSLYPGGLKEVPYRNLFQQKPTQPVY
                     LAVKGMLPKNSLGRKMLKKLKVYAGPEHNNQAQKPQVYEF"
BASE COUNT         8117 a         5919 c         4675 g         9612 t
ORIGIN      
        1 ttcaaaaagt tcattgaagg attttttatt tctcttgtta ttttaatttt acgatggtta
       61 cgccgtcgcc gccctcgttg aaggtcccca gccttgattc tttaatatgc ttgtgttttc
      121 taagaaactg gcttactccc tctcgaagca ctcccgtccc cttgccgtga attacctgaa
      181 cttcgtttaa atttgacata aaggcatcat ccagatattt atcaagctcc atcagtgcct
      241 catcaagatt atatcctctt aagtcaatta caggactaat ggatgaagtt tttagtttaa
      301 ccatcccgga tgtgccggtc ttttgtttaa cttcctcatc agacttaatt ttcaccaagt
      361 ctgctttttt aacctttgtt ttaataagtc ccatttgaac cgtcacagtt tctgaatcat
      421 ccacatcaga aataataaac cccttttgat taatagttct caccatgaca gcatcacctg
      481 tttttaaggg tgtatcctta tcatatgtat cttcttcctc aatttggtca gtgaaaatat
      541 tccccgaaaa ttccctggtc atatcgctta aggtctgcct taattcgttt acacggcggt
      601 ttttatcctt atccatttca aggtttaatt ttcttaattc ctttatgatt tcagaaactt
      661 ctctttttgc attctcgact atttttctgg cttcatagtt ggcatcatcc accagttttt
      721 cgcttttctc aagggacttt ctttcctttg ttaaaaagtc gctgtgtttt ttcttgcttt
      781 ctgattccag gcggttaatt tcctgttttt tttcttcaat ataaattctg ttttcttcaa
      841 tttgctttaa ggcatcttca aattcgattt tgtcagtttc tatgttttgt tttgctcttt
      901 caattatatt ttgttgcagc cccagttttc ttgatatttc aaaggcattt gattttcccg
      961 gcacccctat caaaagcctg taggtggggc ttaatgtttc cacattgaat tcaacacttc
     1021 cgtttattac tccttctgtt gtaaggccat ataatttaag ttctgagtaa tgggttgttg
     1081 cagcagtcag tatccttctt tcatggagag tgtcaagtat tgcaattgca agcgctgccc
     1141 cttccgtagg gtctgtaccc gcacccagct catcaaaaag aacaaatgcc ttttcatcaa
     1201 cctctttcat tatcttcaca atatttgtca tatgagagga aaatgtactc aagctttgct
     1261 caatgctctg ctcatctcct atatccgcat aaacagcgct gaacacggac gtttcagtac
     1321 cgtactcggc aggcagatga agccccgcca ttcccataag tgtaaaaagg ccaagagttt
     1381 ttaatgaaac tgtttttcct cctgtattgg gacctgttat tataagtgtg gtatattcat
     1441 cacccaaaac cgcatcgatg ggtacaactg tttcacggtc aatgagagga tgccttcctt
     1501 ttttaatcct tatataccct ctgtcattta acttgggctc aataccgttt atgcttattg
     1561 catacttacc cttggcaaat ataaaatcaa gctctgttaa tatttcttga ttatttagca
     1621 tttcactgct tatatcgcct atcataaggc tgagttcaag aagtatgcgg tcgatttcct
     1681 tcttttcctc gattttcaaa ttggaaatat cattggtcat ttccacaaca gccataggtt
     1741 caataaataa agtggagcct gttgcagact ggtcgtgaat tatgcctcta accatggacc
     1801 tgtattcttt tctgacagga ataacatacc tgtcgtttct cattgttact attgtttcct
     1861 gcaggtattt ctgcattgat gaagagctta caatggaatt gattttattt tttatggaat
     1921 tattcttaat ttggatttct cttcttattc tcttaagctc agaacttgca tcgtctgcta
     1981 tttcttcctc gcttataata accgaatata ttctttcttc aatacttttg tcagtataaa
     2041 taccttcaca caaagcttcc agcaaatcat aggtcctgtc atcgttgtta agaagaacgt
     2101 agttttttaa aagtctggca gctcttaagg tatcggcgca atttatcaaa cccctgggag
     2161 atataatacc tcccacggct cctcttttca cgtattcctt tatatctgaa attcctccca
     2221 agggaggtga acctttttca atgattattg aaagtgcctg ggctgtctgc ttctgcttaa
     2281 aacttacgcc ttcaaggctg tcagatgtat ctattttgta cacctcttct tttcccttat
     2341 ttgtttcagc aaatccggca actctttcaa taattttatc aaattccaaa acagatttag
     2401 acttttgtat catagtaatt cctttcatgt gtctccatac atttattaaa taaggttcgc
     2461 aggtaaatta actaatttat ttgctaattt atttctactt gctttgcata aattaaaaat
     2521 tttgtgtgtt tgacctaccg ctaattttct ttccctccgg tcagaaaaac agggttaggg
     2581 catctacaat atattcttca ggtagtggcc tcttgtaaat ttgttttcct gtaaaacatc
     2641 aggtatttta accccgttta tttcgcagta gagggcatcg attatttctt ccgcatcctc
     2701 tatattctta gtgtctatga aaaaaaactc aatatttgtt ccaacgaaat catgggcttt
     2761 tccaataatg gtaagaggga cactgttata tatatatgta agtttatttt gtctttcaat
     2821 attgaaatag actccttttc tgtcctttaa catatacttg tttctatagc tgcacttctc
     2881 acaatcctgc aggtttttac agtttttaat catggacata gggcagtttt tcatagccat
     2941 aagctggaca tattcatggg caataatttc cgaactcaag gaagaatgct tcatcatgct
     3001 ttccatgtcc ctgatatttg tttctacaga aaaggtaaag ctgtcaactc ccatttccct
     3061 taacaattct tctgaaaacg tattgattat gttaaaaaat ggtccgcagt gaagcttgag
     3121 ttttgagttt ttcctaaaga aataaaagct tccaacattg ttaacgcata cgccgtcaaa
     3181 tatttcttca tacagcctta ggtttttctc cagaaattta taatctgcca tagatacaat
     3241 gtttggaatc cataaatatt tttcttcgat tttaatattc ttaacatctt ccgtatttaa
     3301 ataataaggc acatacaagc gcttaacctt aatattgtca gcgtatttca attcatcaat
     3361 actgtttatt ttaaggctta acttgggagg ttgggctttt ttattattaa aagaaaatat
     3421 atcattgtag gaaaaagcct taatttctct tctgtcataa accctgccgc atttgtcata
     3481 aaggatttcc acagcatccc gccttaattg attcaaggtg ctttttctaa tgaaaacacc
     3541 ctcatccttt tttatttcca gtccatcaag ttcatatatt gtattaccca gcttagacag
     3601 ctggtcattg attatttcat cagtgacaaa atttctgagg ctgctttcgc aagcctcttc
     3661 actttttacc tcaacagtat tttcaccgtc gaatagcttt agtacagcct gctctccaat
     3721 tttaagttca atggaaaagc ttattttatt tctcctgaac cttgaaaatt cctgtgcttt
     3781 atcctttaca tcctcttcta tttcctcaat attttctccc tttatgccgg agcttaattt
     3841 tgcatacatt tcatcatctt ttgaaggctt taggtatccg tttgtgtaac ccctgctgaa
     3901 tgtctgaatg gctttgtctc tcaaatccgc agtatcagta ttattaccac tgtaataatt
     3961 atcgattatt tgtctgtagt attccaccac tgatgaagtg tatgcaggcc ccttcatccg
     4021 tccttctatt ttaaatgttt taattcctgc ttctataagc tcatatatgc tttcccccgc
     4081 gatgaaatct tttaagctca aaagaggcac tgtattatag tcttcaattt cccggtctgc
     4141 ttccttgtcg taaaagctgt agctcagtct gcagggctgt gcacactttc ccctgtttcc
     4201 gctcctgccg cccaaaaagc ttgacatata gcactgtcct gaatagcagg tacaaagagc
     4261 accgtgtatg aatacttcca aatccgtatt ggtgtttttc gctgtttcct taagctcctc
     4321 tatggacagc tccctagcca taataacctt tttaacattg acttgttcaa aaaacttaac
     4381 accgtattca tcatatacag cggcttgtgt gcttatgttc acgggaaaat caggcaggta
     4441 tttgtttatc aggaaaagta aacccaaatc ctgcactatt aatgcatcaa catcattttc
     4501 atacaaaaat accgcatagt ttaatgcgtc aataatttca ttatccttta atacagtatt
     4561 aagggtaaca tatactttga cacctttgtt gtgtgcatat ttaacaatat gcttcatttc
     4621 ctcgttatca aaattctggg aagactgcct tgcattgaaa aattttcctc ctaaatatac
     4681 tgaatctgcc ccgctgttta ctgcggcata aaatgattct atactgcctg ccggtgctaa
     4741 tagttctgct ttcattttaa ctcctgttta aaaaagaacg ccgacgcgtt cttaatggtt
     4801 tcgcagtcga ccttcacgaa tttatcagct cctttcgctg cataaattcg gaattcgttg
     4861 cgtttgattt accttaattt tccttctccc tttggtcaaa gaaattaagt aaatcatgat
     4921 tttgatgatt cttcgtctaa aagctccatc agagcagcat tatccttttt cagctgattt
     4981 atttccagct ggttttggaa gttttctgct tcctttctgt tcagtttttc ctgcagaagg
     5041 tttacgctgt taattaggct ttccttgtca ctgttcaaca ttaaaagctc ttccaaatat
     5101 ttttcctttt catcattaat tgactcatat atttctttta ctgaaccctc gttgtttttt
     5161 aaatcattta taagtttatc cttggcagct attgtttcat ccttttcatt aagcttattc
     5221 tgcagcaaat tgattttatt tatcaaggat tcaatatcct tttcctttga gtcaaccaat
     5281 aagttcattt tttgctgctc atcgctgaaa ttgtacagca tctgagattt atcatccact
     5341 gagcttttaa gttcccttat atctttttca agcttaatta attcctggtc tttctcgttg
     5401 agtttaagtt gaagctttga aatccgtccg tttaaacttg cctttgtatt gttcataaag
     5461 ctgatttctt ttgtgaagtt ctctctttcc ttggacagtt catcaatatc ttcaagcaat
     5521 tcttcatttt ttatttttaa ttcgtttatt agtacttcac tcttcaaaag ctgctggtcc
     5581 tgctcgctta ctctttcctg gagctttgat atttgtgaat ttaaatattc tctgtcgtta
     5641 tctcttgcca gtaatttttc cttgaatttc tctttttcaa ttattgcgtc tgctgccttg
     5701 cttacagatt cctctttttc cctctcgaat gattctttct cttcctttaa tgtctgaatt
     5761 tcttcgcata tttctttttt gtactcaaca aattcgccat taagctttaa atagctgtcc
     5821 acaataacaa aggccgacaa aaccaaattg tccatgtcat taaaccgctt gttttgatta
     5881 gctattagat ttatctgctc atttaataca gtttcgattt tttttacata gtcgggactc
     5941 tcatcggttt ttacattgta ttttttatct gcaattacaa tttctactct attcatcttc
     6001 aaatcccctt tattctttga ttttgatgaa ataacattca ttatccttct actataatat
     6061 aaatgaaaat ccttaaaatt tcaataacaa ataatacact ttcccactaa tttttcataa
     6121 gagcatagct ttattatttt gatgaagctt ttaccagagc tttaaatatt tttaaaaaca
     6181 ccggatatct ttcgaacaat atttccggat gccactgcac cccaataata aaagaatgat
     6241 tcaccgactc tattgcttca attgtaccgt cctttgaaac agcagctacg ttaaatcctt
     6301 cagctacatt tttcacagcc tgatgatgat atgaatttac aggaatttct tcggtttgta
     6361 aaatatcata taaaagagta tcctttttta tttgaacttt gtgatggggg taggctgcac
     6421 cgttataatt aggcatgtga ccgcttgtcc catccttttg aacattaata tcctggtaca
     6481 aggaaccgcc gtaggatacg tttattatct gggaacccct gcagatacca agcacgggaa
     6541 tattttttgc tgctgctttt tgaaaaagcc tgacttcaaa ctcatctctt atgtggtcta
     6601 tttccttcac ttctttaatg gggtcctcac cgtaaataag cggacaaatg tcctctcctc
     6661 cgctgaagca aaggccatca acaattttca aatattcatc cagcaattca tcttctacag
     6721 taattggaag cattaggggt attgcgcctg acaagatcaa agcctttaaa ttgctttcgt
     6781 tgattgtttc ataactatag ccgtcgtttg tacttctgcc aactgtcact cctattattg
     6841 gtttcatttt ttcctccaat tgattatatc agcagcaatc gagtctgcat ctaatttatt
     6901 ttctattaac agctcatcta ttgagccctg tttaataaat tcatcaggca aagtcttaaa
     6961 cagtacattt gcttccttag ggagaaagct gattatattt gctccgtatg aagcaaaagt
     7021 cagtgcttca tcaattataa ccagatattt tgttttattg acagacctaa gtaaaagttc
     7081 cttgtcaata ggtttaataa atcttaaact aagaacctct gcatcaatat tttccccctt
     7141 taagatttcc ctggtttcaa ttgctgtctt taccattttc ccggctgcag ctattgtaat
     7201 atcttttcct tcttcaacta caagcccttt ccctaaaacc aaatccgagc cgctgccctt
     7261 gatattttca ttcatggctg ctcttggata tcttattgca acaggtccgt caaagaaaac
     7321 agcaaactcc agcatattgc cgaattccct gcaatcagcg ggagataata ccgtcatatt
     7381 tgggatagag cttaaaaaac cttcatcaaa aacaccctga tgagtttccc cgtcatttcc
     7441 tactattcct gctctatcta ctgcaaaaac aacatggagc ttttgcagtg ctacatcatg
     7501 gataacctgg tcgtaagccc tttgaagaaa ggatgaatac agggctacaa caggaacaat
     7561 accgcttgcc gccagccctg cagccattgt aaccgcatgc tgctccgcaa tacctgcatc
     7621 aaaaatcctg tttggaaatt tcagggcaaa gtcctgaagc cctgtaccat cagtcatagc
     7681 agctgttata gcagcaactt tttcgttttt ctctgcaatt tcgcacattt ttatgccaaa
     7741 gtaatcggag tatgacaagg cagaattatt tagaggctga cccgtttcca gattaaaagc
     7801 tgatatgccg tggaattcat tgggtctttc ttcagcaaac ctgtatcctt tgcccttttt
     7861 tgtaattaca tgcattaata aaggaccttc aatatttttt gccgtttcca ggtattcgat
     7921 aagttcatgc atattgtgcc cgtccacagg tcccatgtat gtaagtccca actcttcaaa
     7981 aagcattcct ggcacaacta tttgctttat accgtccttt gctcttttta aaacagattt
     8041 aatctgcctg cccaccggaa ttttatttaa tagtttttcc acatcccttt tagttgtcaa
     8101 atatcccctt gtggttctta ttctgctcaa ataacgggac aggccgccca cattttggga
     8161 tatggacatc tcattgtcgt ttaaaataat caaaagcttt gtattgcttc ttcctgcatc
     8221 attcaatgct tcaaaagcca ttccgcctgt gagagcaccg tctcctatca ctgctataat
     8281 ttcataatct tcttttttta ggtctcttgc tcttgccata cccattgctg cagaaacaga
     8341 tgtgctgctg tggcctgtat caaatacgtc ataaatgctt tcctttcttt tggggaatcc
     8401 gcttatgccg tcatgctgcc tcaatgttga catgtcattt tttcttcccg taagaatttt
     8461 gtgggcataa gtctggtgtc ccacatccca tataatttta tcgcggggag tgttaaaaac
     8521 cttgtgcagt gctactgtca gttctataac acccaggttt gatgcaagat gccctccggt
     8581 ttttgaaacg acttccagaa tatattgtct caattcgctg caaagacggt ttaattcatc
     8641 cacatttaaa tgcttaatgt catccggcgt atttatattt tccaataatt tcattataac
     8701 ctcgctgtgt gcttaaaatg cactataata attatattat gtttgcatag ttatgtcaaa
     8761 gttattataa tatttttgga tttaatgaaa taaatactgt acttttacca aaatttataa
     8821 tattattgac tatataaatt gtttttgaat aaactgttta ttaaacacta aatgttgatt
     8881 tgtcagtatg cgaaaatata attgagtgtg acaatattaa gaaatctaag ataaaaggag
     8941 aacattatgg aaaagaaatg ggtttattta ttcaaagaag gaagcgcttc tcaaaaaggc
     9001 ttattagggg gaaaaggatc aaatttagct gaaatgacta atattggaat gcctgtgcct
     9061 caaggattta cagttactac tgaagcctgc acagaatttt acaatcaagg ggaaaaattg
     9121 tcagatgaaa taatcagcca gattaaatct tatttagcta tacttgaaga acaaacagaa
     9181 aaggtgttcg gggatgttga aaatccgctt ttggtatcgg taaggtccgg tgctaagttt
     9241 tcaatgcccg gtatgatgga tacaatcctg aacctgggnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnga cataccccat gatataggaa cagcagtgaa tgttcagtca
     9421 atggtatacg gaaatatggg agacacatca ggaacaggag ttgcattcac aagaaatccg
     9481 tctacgggag aaaataaggt attcggcgaa ttcttaatca atgcccaagg cgaagatgtt
     9541 gtagcaggaa tccgaactcc tcttacaatc gacaaactga aggaaataat gccggaaatt
     9601 tatgatgaat tcatatctac agctgagaaa ttggaacaac attacaagga tatgcaggat
     9661 atcgagttca caattgaaga aggaaagctt tacatgcttc agacacgttc aggcaaaaga
     9721 actgcagatg cagcattgag agctgcagtt gaaatggttg atgaaggatt gatatctgaa
     9781 gaagaagcag ttttacgagt tgacccaaaa tcattagacc agctgttgca cccaaaattt
     9841 gaccctgatg aacttaaaaa aacggctccc attgcaaaag gacttcctgc ttcacctgga
     9901 gcagcatcgg gtaaaatcta ctttacagct gatgatgcgg tagaagcatc attaaaaggc
     9961 gaagatacta tactggtaag aaaagaaaca tcccccgaag atattgaagg tatgagcaaa
    10021 gcaaagggca tactcactgc aagaggaggg atgacttctc atgctgctgt tgttgcaaga
    10081 ggcatgggca aatgctgtgt tgccggctgt gaatcagcag tagtagatga aaagaacaaa
    10141 accatgacag taaacaaggt tgtttacaat gaaggcgact atatttccct tgacggctca
    10201 acaggaagcg tatatgtagg agtaataaaa acagttcctg ccaatatatc cggaaacttt
    10261 gaaaaattaa tggtttgggc agatgaattc agaagactta aagttcgtac aaatgcagat
    10321 acaccccatg attcagaaac agctgtaaaa ttcggagcag agggcatcgg actttgcaga
    10381 accgagcaca tgttctttga agaaagccgt atattctctg taagaaagat gattatttcc
    10441 gatactttgg aacagagaga aaaagctttg gcagaaatta tgcctatgca gaaaaatgac
    10501 ttcaaaggaa tatttaaagc aatgggcgtt cgtccggtta caataagact gcttgatcct
    10561 ccccttcatg aattcctgcc ggtagaggaa gaagatatag tggaattagc tgaagctatg
    10621 gatgttccgt taaaagaatt aaaagccaaa atcagcagtc tccatgaact taacccgatg
    10681 ctgggccaca ggggctgccg tcttgcaatt tcataccctg aaatagcaaa aatgcaggca
    10741 agagccataa tcgaggctgc cattgaagtt tcaagagaag aaaatattga aattgtgccg
    10801 gaaataatgg ttcccctcgt aggcaagaaa gaagagcttg tgatactgcg aaaactgata
    10861 attgaaacag ctgaggaaac aaagaaagaa ttcaaatctg atctgaacta tcttgtaggt
    10921 actatgattg aaataccaag agcatgcctg acagctgatg aagtggcaga atatgcagat
    10981 ttcttctcct ttggtacaaa cgaccttaca cagatgacat ttggatattc tagagatgat
    11041 gcaggcaaat tccttgaaga ttacaaggaa gcaagaattc ttgaacaaga cccctttgaa
    11101 acaatcgacc aggaaggcgt gggaaaactt gttgccatgg ctgttacttt aggcaggaaa
    11161 gcaaaggaag agctgcacct tggagtttgc ggcgagcacg gcggagaccc cgcatcagtg
    11221 gaattcttcc acagggttgg gcttgactat gtatcatgct caccgttcag agttcctgtt
    11281 gctagactag ctgctgccca ggctgcaatt aaagataaac tgaatgttca tattgacaaa
    11341 tagaaaatag caattaaaag gggggccgct caaaactaga gcagcacccc ttttttctaa
    11401 agtaaaaacc attatatata attaaaaact cctaagaaag aaaaaatctt ctctaaaaac
    11461 tgttgacgtt gattggcaga ccacccgtcc ccttttcata tgccagattt ttgtaggttg
    11521 tgaatatgaa tcttaatccc tggtgttcgt gcagctcaga agcatacgtt agaagccagt
    11581 ccttatcctc ttcaaaatta tgcaaatatg tatctgcttc caatactgcg tcaaatttgg
    11641 taatataggc ttcgctgcaa aaaggataca attcattgta caattttccc ccgcctatta
    11701 aaaacacatc gtcactgtta taatttttca gctcttcaaa caaggcttct gttgaattta
    11761 ctacgataca gccttccttt tcaaaattca catcacgtgt taaaactata tttgtcctgt
    11821 ttatcaaggg ttttcctccc ggaagagaaa gaagagtttt tctccccatg acaacaacct
    11881 tattcaaggt agttttttta aaaaaatcca tatcctgagg cacattgaat aagagctccc
    11941 ccttatttcc tattccccaa tctcttgcta ctgcaactat taatttcatg gtttctccct
    12001 tataccgcaa tgggtacctt taccttaaaa ggattgtatt tataatcaac taaattaaaa
    12061 ctgtccacag aaaaatcata aaaatcattg atgctcttgt ctatttccag aaccggtgca
    12121 tcgtggcttt ccatttcaat tagctcttct attataggaa catgcctgtc gtaaatatga
    12181 gcatccgcaa ttacatgaac cagttctccg gcaaccagtc cgctaacctg agccatcata
    12241 tgcaccaata ttgaatactg taccacgttc cagttgttgg cagccaaaac atcctgagac
    12301 cgctggttta gtattgcatt taacctgttg cctgaaacat tgaaggtcat gctgtatgca
    12361 caagggtata gatgcatttc atgaagatcc tgaaagttat aaatatttgt catgattctt
    12421 ctgcttgcag gattgttttt taaatcatag attactctgt ctacctggtc aaaatcccct
    12481 tccctgtatt tatgcttaat gcctaattga taaccgtaag ccttgcctat ggagcctgtt
    12541 tcatctgccc atgaatccca tatcctgctg ttcaagtcat taacattatt tgacttcttc
    12601 tgccatatcc acaataattc atctacggca gctttgaaat tagtttttct cagtgtgaga
    12661 atcggaaagc tctcctgcaa atcatatctg tttaccactc caaacttctt gatagtgtga
    12721 gcaggcgtac catcctccca ccttggcctt acatcgtaag ctgtgtccca tactccattt
    12781 gtcaatatat ccttgcacat ttgtataaaa attttatctg cagtactcat aatttatccc
    12841 tcactttaaa tattacagat attatatatt agaagagttg ggattacaag aaataattgg
    12901 acataaagaa ccaaaaagga actgtaaaag caagttcctt cctatctcat ggtattttgt
    12961 ctgataaaat tttttatctg cattttttgt tcaaaatctg atactctcaa ggcttcggaa
    13021 tcctcattgt caaaattctt aaaatatatg cacatgtccc cgtctttggt atcatcatat
    13081 cttatgcacc tgaatccatt atcgtacatt ttttggattt tctcaaaatc attcataatt
    13141 ttctcccctg gtgttttgta tatattattg ccactatata gaaaattatg actaagagaa
    13201 aaaaataagc ccgatttatt ttcaatcaca ggcttacgct gtcctaagct ctcttgcacc
    13261 ataatatttc tcatctaatg cttcaagcac atattcatca aacaatgtgc cggcagaagc
    13321 aacaatgaca ttggctacat ccttcaaatt tattttgctg cttacacctg acaatacccc
    13381 tactcctatt gcaccggaat ttaatgcaaa gagcatatcg ttataggaat ctcccactat
    13441 tgccacctgg ccgggttcca agccgtacat tgagcaaaat ctcatacaca tgtccttgtg
    13501 aggctttcgc ttcattgtac cgtcatatga gccgatgaaa tcaaagctgt cgcttaaatt
    13561 cagggtgttt atcatatgca tgacggattg gctggaatct gctgttgcca tacccatttt
    13621 tatgtttttg gaatttagat attcatataa tttttttatg cttacaattt ctttaaaatc
    13681 agcatcatcc tttaatactt cttttcgaag caattcgacc acatgggtct ggaatctgtc
    13741 aagttcgata ttcacatgct tttttagaat ggaatgaagc tcatatgcca cattctcatg
    13801 tgagctgtaa gctaaagctc caaaaggctc cactccattt tcctttatgc cgatagctct
    13861 ttgaaggtct ttgtttagct tttcgccatt tatatcatat tcattcaata tagtttgaat
    13921 cgttttgatt ccggcattcc tccacaatga aaaatcaatc aatgtgccgt ccttgtcaaa
    13981 taatattcct ttaaccattt tcccaccccc ttttttgttt tgtattgata atatattaca
    14041 cctggtttat atgatttctt tgttcatatt ataaaatatg tgtatataaa acannnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncacctgg tttatatgat ttctttgttc
    14221 atattataaa atatgtgtat ataaaacaaa tgcacattta aacattctac gtaatgaatc
    14281 tttgcctatc cttaaaaaga attattgaga ctactattat attttgagga atcaaaagtg
    14341 tgacagtaaa agtataattc catactgtct gccaaaatac cggcaataaa ggaaaggagt
    14401 ttatatgaga gtacagaaac acacagggtt tatataattc caagtcgggg aaaaagaaaa
    14461 agactgcgag gattcctcct gtacagtcta tgatttaata ttataagaaa agttgcgtaa
    14521 tttgtttaat caaggaaatc aaattcaact ccgcagacac ttattaagtc tccctctacg
    14581 gcacccaggt ctcttaattt ctgtattact cccttgtcca caaggacctt ctggaaattt
    14641 gaaaaggatt caaaatcatc aaaattggtt gatgcaacaa gtctttcgag gaagtctccc
    14701 tctgcggaaa aataatcatc ttccttgctt acaacaatcg tatccctatc ctttttgtca
    14761 aagaaacttt ctacagcatt ttcatcaaca aattctatct cttcttcaat ctggatcagt
    14821 ctttcatatg ctcttttctt caaaaagtca agtccctggc cagttgcggc agatacttgg
    14881 attatttcgt atttgtctga aaatattttg tttatctttt caacattatt tgcagcttcc
    14941 ggcaaatcca ttttattcaa gactataacc tgatgttttt gtgataattt ttcgctgtat
    15001 tggcttaatt cttcatttat tttataaaaa tcttcaaccg ggtcccttcc ctcctggcct
    15061 gatgcatcaa gcacatgcag aagcaatctt gttctttcaa catgccttaa aaagtcaagc
    15121 cccaagcctg caccttctga agcaccttct ataagcccgg gaatatcagc aagcacgtag
    15181 ctgtggcctt cgcctattga aacaaccccc aagttcggct ttaaagtcgt aaagggataa
    15241 tttgctattt ttggcttggc gcttgttaca gatgccagta ttgttgattt tcctacattg
    15301 ggaaacccta taagccctac atccgcaatg agcttcagtt caagcacaat ttccctttca
    15361 tctcccttgc ctccaggttc ggcaaatcgg ggagtgcgcc ttatggagct tttgaattta
    15421 acatttccct tgccgccgtg gcctcccctt gcagcgacat attcctgacc gccggttttt
    15481 aaatcagcca gcacaatgcc tgtttctttg tcctttatca aggttcccgg aggaaccctc
    15541 aaataaaggt ccttgccgtc agcaccatat tgttttttcc ctctgccgtc ttctccgttt
    15601 tcagcctgat aatggttttt gtatctgaaa tccatcaaag tcctgagacc ttcatcggca
    15661 actatgatga cacttcctcc gtcgcctccg tcaccgcctg caggcccccc cattggtatg
    15721 tatttttccc gtctgaatgc gacgcttccg tttccgcctt tgccggcctt aatgcttatt
    15781 tttgccatat ccacaaacat atttttattt cacaaccttt tttattagtc ttcccttata
    15841 cctgtaatta aaaacctacc ttacggtagg tcgtttattg cgcaagcaac aaggtttccg
    15901 ggtacccaac agcaatctat gattgcgtcg ttgggttggg ttcttattat gcttctaatt
    15961 caacaggata tacgctgacc tgttttcttg ttttatcttt tctttcgtac ttaacctttc
    16021 cgtcaaccat ggcaaatagt gtatcatcac ttccaatgcc tacgttaatt cccggatgaa
    16081 gcttggtacc tctttgtcta acaagaatat ttccggccaa aacaaactgg ccgtcggttc
    16141 tttttactcc caatctcttg gaattgctgt ccctgccgtt tcttgaactg ccgacaccct
    16201 ttttatgtgc aaataactgt aagtttaatt tcaataacat ttttacacct cctcaaattt
    16261 aagtgtaata taatcgctat aatcttccaa taataattcc attccgacta tgagatttct
    16321 gtatactata tcagttttat cattgttctt aagtgttttc aaactcatca tcccctcatc
    16381 atccactgca tattcaatgt tttgaggttc tatcccggca acaaggttca atgaatttaa
    16441 tgctgtataa gacaaaatcg aaactgacgc acaaattatg tcaaatcctg cttcggcgta
    16501 atcagaatgg cctttaacca taaaaccttt gatttcatcc ctggttttaa atatggtaac
    16561 tgtaatcata ctaacctact attttttcaa ttttaacctg tgtatagggc tgacgatgac
    16621 cttgtttttt tctgtagtcc ttttttgatt tgtatttgaa aattaatatt ttcttttctt
    16681 ttccctggtt ttcaactact ccttcaacta ctgctccgtc aacatatggt ttccctacat
    16741 tcagctttcc ttcatcggca acaagcaaaa ccttatcaaa ttttactgtt tctccgtctg
    16801 cgatgctaag tttttcaact cttaccacgt cgccttcctg aactctatat tgttttccac
    16861 ctgtctcaat aattgcgtac ataagctacc tcctcttccc agtctcgcca ttgcaggtac
    16921 ttcgtgtttt aaacctgttc cgagcggctt ctaacaaata atgattctat caaaatgtaa
    16981 aatgtttgtc aactaatttt tttgtcgcag cacaatccca ttttgcacga ccgcagagag
    17041 tgaactaaat ggtgatggag cctgcgaaac tgctatattc gatatattcc atctatattg
    17101 gacaatttcc ccattttccc tgcaataatt tcatctgata aaatatgctc atcctgttca
    17161 atatatattt taatatcgta ctttttttca atttcagcaa taatgtccat gcagcttttg
    17221 actattttat tgtacaataa gcatcccaca tttaaaacta ctgcttccga tgatgtatta
    17281 agcttaatat tctttattat ttcttctatt tccataaata taaatgcttt tgacatgctt
    17341 ctgccgctgc cgctgcaatg tacacattcc tcggtcataa gattatagaa attttcctta
    17401 ttcttctttc ttgttatttc cataatgcag agctttgtta tccctataac attggttctg
    17461 gttttgtcat ccttaaacag ggactttgct tttgctttta acagggcaat actttctttc
    17521 tttttaatat caatgaaatc aattaaaata atcccgctga tatttctaag ctttacctgc
    17581 cgtgctattt cttcgactgc ttctaaatca attgccaggg ctgtttcctc catattgcgg
    17641 cttcccgtat atttcccgct gtttacgtca atcaccgtca atgcttcagt catatcaatt
    17701 attattgagc ctccattttt tagttcaacc ttcctgtcaa gcagcttttt aacctggttt
    17761 tcgatattaa atttttcaaa taccctctgt tccagtatta ctttgatatt tttaagatca
    17821 ttcccggaaa ggccggaaat catatccttg aatctatcct tagtaatttt gtcttccaca
    17881 taaatctctt ctacggttga atcgatatag tccaaaaaaa gtctttcatt cagagaattg
    17941 cttttatata gaagcttggg ggcataggaa tatttgtatt ccctttcaat ctgcatatat
    18001 atgctggaca gcatctcata ttccttttct attgcttccc gtggacatcc cttggcaaat
    18061 gttcttataa tcatgccatt tccgtcagcc attatgcttt tgcctatttc ttcaagcctt
    18121 ttccgctcat cgcggtcttt gattttctta gaaagattaa ctcccatagt attgggaata
    18181 aatgcaatat attttcccgt aaaggatatg tcagttgtaa ctgagatgtt tttctcacct
    18241 atgggctctc ttacaatctg aaccagcagc tcatccccgg ttttaagcac cttgttaata
    18301 tttttagcat ctttcctctt aatccttaat tcttgcagga acttatccga aagcaggtca
    18361 tcaaaaaaca aatatgcatt cttgccaagg ccgatgtcaa caaaggcagc ttccatgccc
    18421 ggtagtatat tcattacttt tcccttgtag atgttacctg aaacattttg cttgttattt
    18481 aattcaaaat gataatcaac aagcctgcca tcctgtataa ttgccgcatg attaaaaaat
    18541 acagaactac ttactaaaac ctgcttcatt gcactctccc cattactgca atattggctt
    18601 catatcttta tccaatgcat ctattttcat cactgtgaaa taatctgttt caaaaccttt
    18661 tttccttaat aatttaataa gctcttcgac cttcatgctt ccgctttcct gagttttaaa
    18721 aacagcagca attttggttt tattatttat attttcagcc tttaaataat gcagcagatc
    18781 tttggtgttt aattccctgg tcactgattt gtttttttta tttttcttat ccctgtaata
    18841 tatttcccct attttaatca tttcatctaa atatttatta agcttatcta tatcgatatc
    18901 cgactcaata tttatcgaat actgggcata ggcgcatctt ttcatgagag aatctgtatt
    18961 ttgggtataa cctgccttga ttacctgcat ttccttaggc atcaccatgt tcatcttttc
    19021 tgtaaagagg tttgggtcta tactttcatc aagctccagt tcaaaatatt cacctgtgct
    19081 ctctattccc agagacaagg gataaccgaa ggatgttttc atgtgagggt taaagccttc
    19141 tgagtattta gcatttatac cggctctttt aacagacctt tggataaatc ttaaaacatc
    19201 caggtgggaa atatatttta aatatccggt ttttgtgtat ttgcatatta ttttgcacat
    19261 gggcaggcac ctcccagcaa attatagtta accccgcagc ttgagcattc tattctgcag
    19321 tcgggagtgg tggcacaatc aagtgcattt ttatattcgc taattaaatg tttcttggtg
    19381 actcctatat ctatgaagtc ccagggcaaa acttccgtca attctctttg ccgcagggca
    19441 aaaaagtcag ggtctgtatt tgtttcttca aaggcatcca tccattttcc aaagtcaaac
    19501 atatcgtacc atccgtcaaa ccgacaacct gattcccatg cctttatcaa ggctctggaa
    19561 agtcttctgt ccccccttgc aaatacagcc tccaaataac ttaacctggg ttcatgatag
    19621 ctgaatgaaa tcttcttatc cttgatttcc ctttgcaatg cctttgcctt atcatagaac
    19681 tggtccatat tgttttgagg aaaccactga aaaggtgtaa aaggctttgg tacaaagcag
    19741 gctgcgctta catttatttt caaatttccc tttatgtctt ctataggtct gtggaaaaac
    19801 ttatccttta ccttgtagga aaggtctttt atccccagca catcttccat tgtttcgcct
    19861 ggaagtccta tcatgaaata taatttgacc gtagaccagc ccaggttaaa ggctgtttcc
    19921 acaggcccga gaatttggtc ttctgtaagg tttttgttta taacatctct catccgttgg
    19981 gttcctgcct ccggtgcaaa tgtaaggcct gattttcgaa cctcctgaag cctttctata
    20041 atatcaaatg tcattgaatc aagccttaat gaaggcagag aaatgcttac attctggttt
    20101 tcgtaggtat ccaaaagttt caatgtcaaa tcaggaaggc atgaataatc tcccgagctt
    20161 aatgaagtaa gggatacttc atcatgaccg gttgacctta aaagactgtc tgcctgttct
    20221 aatagcgtat ccgccctttt ttccctgaca ggtctgtaaa ccatgcctgc ctggcagaaa
    20281 cggcatcctc ttgtacaccc tctgaataat tccaaaacca cccggtcatg aacaacatca
    20341 atgtagggca ggatgctttt ttcaggataa tagcttttgt ccaggtccct tataatccgc
    20401 ttcctgataa tttctttggc attattgttc aacagtcttc tttcctttat tgtaccgtca
    20461 tcattatata tttcctcata aaacttcgga acatatacac cttccaaggc tgctgctttt
    20521 tccagaaaac tattcttttc atatcccttc tttttttcat cactgtataa atttataagc
    20581 tcgggtaaaa cttcttctgc ttcgccaatt acgaacagat caattatttc atggagaggc
    20641 tcaggattat aagcacaggg acctccggca atcaccaggg gcattctgct gtctctgtcc
    20701 tttgacctta tgggaatgtt tcccaggtct agcatgttca aaacatttgt ataactcatt
    20761 tcatactgga gtgtgaagcc tatgaagtca aaggctgtaa gttcatcctt gctctccagg
    20821 ccgtacaagg gtatcttgtt tttacgaagc acctcttcca tatctgtcca tggcgcaaac
    20881 accctttcgc accatacatt ttccatgctg ttcaataagt ggtacaaaat atgcagtccg
    20941 gtatgagaca tccctacctc ataaatatcc ggaaatgcaa acgcaaatct tatatcaata
    21001 tctttatttt ttataacaga gttcagctcg cttcccacat aacgtgcggg cttttccgca
    21061 cttaaaagct ctctgtcaag ttttctttta tctatcattt tttcctttcc ttttacggca
    21121 gtttaagttg ccatattcta gaatatttta tcatattcaa ttattattga caacttttaa
    21181 ttatttaata gtaatgctcc atcgattatt taagcgcagc gagtttacag gctgtaggat
    21241 ttttagcggt catgagcttt agaattattc gctgccgaaa ccgtgagcgg atatttacat
    21301 ctgcttttta aaataataag aatattaagc gaacatttgc aggacacgac tataattctt
    21361 agcgaatgaa gattaaaaat ccgttaatca gcttgcactg tttgagcgca gcgagtttgc
    21421 aggctgtagg atttttagcg gtcatgagct ttagaattat tcgcggccga aaccgtgagc
    21481 ggatatttac atcagctttt gaaaaataat agaaatatta agcgaacatt tgcaggacac
    21541 gactataatt cttagcgaat gaaggttaaa aatccgttaa acagcttgca ctgtttgagc
    21601 gcagcgagtt tgcaagctgt aggattttta gcggtcatga gctttagaat tattcgctgc
    21661 cgaaaccgtg agcggatatt tttattattt ttttataaaa acaatctgga aaaattgtat
    21721 ttaacaattt tattgaaaaa gaagtaaaag tattatagaa ttaaaacaat aaatttagtg
    21781 aggttaatat gaattacgaa gaagcgattg aatttattca ttccacatat aaattcggct
    21841 caaagcttgg tcttgaaaga ataaaaaggc ttatggagat gcttggcaac ccgcataaca
    21901 gctatgaaat catacatgtg gcaggaacaa acggcaaagg ctcaacctgc agcttaattc
    21961 atgaagttct catagaatca ggctacaaaa caggtttgtt tataagcccc tatttggaag
    22021 agttcactga aagaattcaa ataaataaaa gtcacatagg caaaaattct ctggcaagaa
    22081 ttacagcact ggtaaaagaa aaagtcgatg taatgatttc ggaaggatat gaccacccta
    22141 ctgaatttga ggtagtgaca gccatcggct ttaagtattt tcaggaggaa aatattgatt
    22201 ttttagtcct tgaagtgggc ttgggaggaa gatttgatgc aaccaatgta atagaaaaac
    22261 ctttggtctc tattattact tccataagct atgaccacat ggaacagctt ggagataccc
    22321 tggaaaaaat agcctttgaa aaggctggaa taatcaagga agattcatct gttgtaatct
    22381 atccccaggc agaaaatatc aaaaatgtta tcaaagaaaa agctgaagaa aaacactctt
    22441 tagtttacga accggatatc aataatattg aaaaaatcag ctctgatatt accggccaat
    22501 ggtttaaata cttaaaatca gatgtgtttg atctgccggt aataaaaatt aatctactgg
    22561 gtagccatca gcttttaaac gcacttacag ccctttccgc cttggagcta attaaaaaac
    22621 aaggctgcag cataagttca gaaagtataa tcaatggttt ttcaaactgc aggtttcccg
    22681 gcagatttga aattatcaac aaagatccct taattatcct tgacggcggc cataatatcg
    22741 acggcataaa ctcttttaca aagacattac gggaatattt caaggacaag gcaacatttt
    22801 tctttggtat tttaaaagat aaaaaacctg tggaggcttt ggaattattg ctgccttatg
    22861 caaaggaaat ctacaccttg actcctttga gtccgagagc attgaaagca gcagaattag
    22921 ctgaattaat taaaaaacac acaaaaataa aagttatccc tttggataac tatgaggata
    22981 tactgcctct gcttggaaat gcaggcaaaa gtgacaccat tgccttttca ggctcactat
    23041 atatgattgg cagcgtaagg acactgttga gaaataacgg ttttgcaaat aacatcaaaa
    23101 atctataagt cccagactga tttttaagca gtcatccacc tctcccataa ggttcttgtc
    23161 aaagcgtcca atcttttcct tgagacgctt tttatctatg gtccggattt gttcaagcag
    23221 gacaacagag tcctttggaa ggctgaaatc ctcagaactg atttcaatat gagttggcag
    23281 cttagcctta ttaatctgag acgtgattgc agaaacaatc actgtaggac tgtatctatt
    23341 cccaatatca ttttgtacaa ttaataccgg tctgaccccc ccctgctcgg aaccgattac
    23401 agggctaaga tcggcataat atacatctcc ccttttaaca atcaaaacta ttcacttcct
    23461 aataatttag tttcatagct taaaaagtcc tcatagtccc cttctgagta ttcctcagta
    23521 atactcttgt tgattttact catttccaga tagccggcct tcatcttgtt tctgatttca
    23581 agcttccttt tttcaatcag ataaaacctg atggattttc ttataaagtc gcttctgttt
    23641 gttccttctt cttttacagc attatccaat tccctcaata agttttcagg gatagaaatc
    23701 agtacttttt tattgtccgc caagatacac acctcgcaat tgcacagtta ttttatattg
    23761 taacacaata agcagataag ttgtatggaa acatttgtat tatccgtaaa tatttattag
    23821 tattattcct caatttttat tgtttcatac tgtatagctt ttaccaattt atcattatta
    23881 taaatataaa ttgtctttgg aaggagattt tctttgctga aatttaaaac atgcttatat
    23941 tctccgtccc aataaatcaa ttcttccgga atgttttcca atatatcaaa ttttctgaaa
    24001 tcaaaatcat gaaggagtgt cttatttaac ttgtaatttt caagtgttac actgctttca
    24061 ggaaatttat cgttgtttaa tatcaatctg ttccctgaaa cataggcata attccaggat
    24121 tcatcttccg attccgttct gatgctgtaa tttccgtccc ttaagataat atttacttta
    24181 tagtcatttt ctgttttatc agtgtatacc ttcatcaatg cttcagctga ataattaaca
    24241 ggcaggatga aggggttatc ttcggcggcg caaccgctgg cagcaatcat taaaaccagg
    24301 cacaaggcaa tcaatctcat aataagacct gcttccatca aaattcaggg atataaagtg
    24361 tatcccccaa atacagggac atttcttcat ggacttgctt cgcaggctca cctgctatga
    24421 agatatgtcc ctcttcctac tactacttta tgatggaatt tttaaatata tgccttaaat
    24481 ctcattcggc tatacactga gcaagggcaa attcactggt gtgagatatg gagatatgga
    24541 tatttttaat attcagctct tctgctcttt ttaaagcatt ccctgacaaa acagcatagg
    24601 gtttgccctt atcatccttt aatatttcga tgtgtaaggg accaaaatta gaaaatcccg
    24661 tgcccagaca tttggaaaca gcctcctttg cggcaaacat tcctgcagct gtttggggat
    24721 tgaatttcct cgttttcaaa tatttcaatt ccttgtctga ataaatcttt ttgagaaatt
    24781 ccgtgttttc catattcttt tttattcttt cgacttccgt aatatctatt cctgtaccta
    24841 taatcaaaat tcctcccccc tcctaagtac gtggacattc cttcaaaggc tatccctcat
    24901 agaatctccc aactttaaag gtgtgtcccc cttccttata aaaaatcaat tgacaaagag
    24961 ctttttgtat ataataatta tattacatgt ttacctgcaa tacaattcca attaatttac
    25021 atatacaata aaggagcccg ctatgaacaa tgtaacagta gtatatcatc cattgattca
    25081 gcataaattg acaatgctga gagacaaaaa cacacccccg aaggatttca gggaattagt
    25141 aagagaaata tccatgctca tggcatacga agtaacaaga agccttcctc taaaagatat
    25201 cgatatagaa acacctatgg ctaaaactac aggcaaaaca ctatccggtg aagatatagc
    25261 aattgttcct gttttaagag caggtctcgg aatggtagac ggtatgcttg acttaatccc
    25321 taatgctaaa atcggccaca tcggtttgta cagaaatgaa gaaacccttg agcctgttga
    25381 atactactgc aagctgcctg cagatattga acgcagaata gtgattatca cagagccaat
    25441 gctgtcgacg ggaggctcaa tgataggtgc attggatatg cttaaaaaaa gaggagcaaa
    25501 gaaaataaaa tcaattcatc tggtatgtgc tccggaaggc ttgaaaagag taactgaagc
    25561 tcatcctgac gttgaaatct acacagcagc aatcgatgaa aaatacgatg aaaccggcta
    25621 tatagtaccc ggtttgggag atgctggaga caggctcttc gggaccaaat aaagatgccc
    25681 tttatgtcat aacctcatgc atgatagcaa aaacccgctt acagtataat actcaggagt
    25741 aactatgaga cacatattta taattaatcc cattgcagga aacggcaagc tgcaagaaan
    25801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgagacacat atttataatt
    25921 aatcccattg caggaaacgg caagctgcaa gaaaaattag aagaatccat acaccttgaa
    25981 ttgaaagata aagaaatcga ttatgaagta tatactacca aatcaaaaga agatacaaga
    26041 aactttgcgg aaaataaatg cagggaaaac ctgccgtctg ttttatattc atgcggcggg
    26101 gatggaactc ttcatgagat tataaatgca gcatttggtt ataagcatgt gagtatcggc
    26161 attatacctg taggctccgg caatgatttc attaagaatt ttacaaacaa ggctaacttc
    26221 aatgatatta ccctgcagac agagggtaaa agcctggaat tggacttaat aaaggcaaat
    26281 gacgaatata ctgccacagt attaaatata ggacttgatg ctgatgttgc ctttaacatg
    26341 aataaattca agaagatacc tttcattcaa ggtccgacaa gatactatct ttcaatatta
    26401 tattctttgt tcagtaaatt gggaaaacaa attcaggtta aaacggacaa tgattttata
    26461 aatggcacat ttataatagg tgttgtggca aacgggcaat attacggagg aggatacaag
    26521 tgtgctccaa aggcacaatt aaacgacggt atccttgacc tgtgctttgt tgagaatatt
    26581 tcaaggctta aaatacttag tctgctggga agctacaaaa aaggtgagca tctggaaaat
    26641 cctaaaataa gcaaatatgt tacctatgat aaaatcagcc gggctgaaat aaattttcat
    26701 ggaccaacca atgtatgcat ggatggagat aaatatattt acagtgaaat aaatatatca
    26761 acagaaaaaa atgccctgaa tttctggctt ccaaaagggg tggaaattat ttcagcagaa
    26821 tatgtgaagt ctaactagtt tatttttggt atactatttt ggggacggtt ctatttgatt
    26881 tggggacggt tccttttgtt ttgcgtgttt tggggacgtt tagggtttta attggacggt
    26941 tttatttcaa gaacgaaaag aactgtcact aaaattctaa aatttttgaa atgacagttt
    27001 ttctgttaaa acactatttt tcattattga atttacaaca acagtacctg caagagaaac
    27061 tactgtgaac tcccctgccc ctacgtaaaa tgcattcaat aaaaatggag tgttaaacaa
    27121 ataggttaat tctgctccaa ccaataaggc gtttgaaatt acaggtccca ggcttgctaa
    27181 aatcagcgct tttttattca atccaaaaag ccttctcacc ttcactataa ataccagggc
    27241 taaatatgtt gcaaatgaac ctgcgagaat gtcaatcagc cccagggggc ttgccaggtt
    27301 tgccagtatg catcccagta ccaggccaag accgtatccc ggctctataa atgcaaacag
    27361 caccaatatt tctgatatcc gaaactgtat ctgctcatag ctaagacctg cgaaagcata
    27421 ggtcaaagcg gcatatgcag ctgcaatgag ggcggtacgg gtaataaact gattattctt
    27481 tttcatcaaa aaaactcctt tcaataaatt cccggagtca ctaaaaaacc ttgatttttt
    27541 aagtgggtgc caagaaaaca cttgattaat ttaacggttc tttttgttcc tgagaacaaa
    27601 aagaaccgtc cttgaaattt taatatttta tctcttgctg aactgtgatg ctcttcttgc
    27661 ttttttcaaa ccgtatttct ttctttcctt cattcttgaa tctctagtca ggaatccggc
    27721 ttttttaagc aggggtctta attcattgtc tacctgcaat aaggctcttg atataccatg
    27781 tcttatagct cccgcctgac ctgtatatcc accgcccttt acatttacca atacatcgta
    27841 ctgacccatg gtatctgtaa tcattaaagg ttctctcaca acatttctta aagtctcgta
    27901 tttgatataa tcttccaagt ctctttcatt tatattgatt ttccctgttc cgggaaccaa
    27961 tcttactctt gctattgctt tttttcttct gcctgttcct ctgtactgtg cgtccatcat
    28021 cgtcctcctc ccttattaaa actcgtatac ctgcggtttc tgagcctggt tgttgtgctc
    28081 gggacctgca tatactttta gtttcttcaa catttttctt ccaaggctgt tctttggaag
    28141 cattcccttt actgccagat aaaccggttg tgtgggtttt tgctgaaaca ggtttctgta
    28201 tggaacctcc ttcaaaccgc ccggatatag agagtggtgt ctgtataatt tctgatctaa
    28261 ttttttgcct gtcaatacaa ctttttcagc atttactact attacatagt ctcctgtatc
    28321 aacatggggt gtatatattg ctttatgttt acctcttaaa agtttggcaa cttccgttgc
    28381 aagcctgcct agagtctttc cttctgcatc tatgacatac caagcccttt caacttcatt
    28441 aggtttagcc aagaagcttt tcatttattt tcctcctgta attctgatgt ttattttctt
    28501 atattcaaaa aacctaactc cggggcattg gaacggtttt tctctttcta ataataccta
    28561 tgacattcta tatgaataaa aactaattgt caagctattt tttataattt acatccatta
    28621 aaa
//
LOCUS       DAOPUT010000014        26053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_1866_length_26053_cov_18.916647, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000014 DAOPUT010000000
VERSION     DAOPUT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 26053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26053
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1866_length_26053_cov_18.916647"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..591)
                     /locus_tag="PKK75_02710"
     CDS             complement(46..591)
                     /locus_tag="PKK75_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ82537.1"
                     /translation="MKELSMHILDITMNSIKAEASLVEIKIEDSKKNNNLTIAISDNG
                     NGMSEEVINKVTNPFYTSRTTRKVGLGIPMLKEACERCSGYLKIESKVGLGTTVFCSF
                     ERDNIDRAPLGNMGETIMTIINSADNIELLYNHKTDSGEFTFDTREVKEMLDDIDIND
                     ISILLWIKEYINENVNIVLNR"
     gene            complement(588..1301)
                     /locus_tag="PKK75_02715"
     CDS             complement(588..1301)
                     /locus_tag="PKK75_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ82538.1"
                     /translation="MKYYYDLHIHSDLSPCSSSDMTPNNIINMSLIKGLNIISITDHN
                     SAANLPAAMELSGKAGIKVIPGLEVTSKEEVHVLCYFKELKNATEFGSIIYDSLPLIK
                     NNIEIFGEQNIYNSKDEITGTLEKLLINASKYTIEDIYSIVKEYRGVMIPAHINKMTN
                     GILGVLGFMPFNLKIEHVEVYSKVPISDKTIEKYKVLYNSDAHRLVDISEAINYFDLN
                     NIDDVYEFLGMRTLYMEEL"
     gene            complement(1298..1630)
                     /locus_tag="PKK75_02720"
     CDS             complement(1298..1630)
                     /locus_tag="PKK75_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNZ82539.1"
                     /translation="MNCLEFAEKLQLTPLSEKEGNIEIEGVYIGDLLSIVMSKAKKNY
                     VWITIQTHINIVAVAELIELACIIVVEDMDVDNDTIEKAKELGIPVFKTSESAYQMAC
                     RMSELGIR"
     gene            complement(1645..2886)
                     /locus_tag="PKK75_02725"
     CDS             complement(1645..2886)
                     /locus_tag="PKK75_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNZ82540.1"
                     /translation="MNFSIMFDENKCKGCTNCMKRCPTQAIRLINNKAFINISKCIHC
                     GECIKICPYNAYTPESIEWVDQMHDTPEKYKIAIPTTSLYGQFPRGTDICKVQNAILK
                     LGFDFVYDASWSAELVARAIKLKIEKEKTVRPYISTYCPAAVRLIKYRYPSLMDNLIL
                     LREPMEIAGILSRERARKKFNLKDEDIQVFYISPCPAKLLAVTNPIGDYKPSVDWVIP
                     LNKIYGELYREVIKEDNITCSYPSLSGMKWAVAGGQSENAGLNNYIAVHGMENIIEIM
                     EEIENGKLSDIDFVEASACVNGCVGGTFNVVNPFIATSSINYISNSLGDECINPDIDR
                     FDEMYESGFFNYYLKEEESSKTLNLKEAVEKDERIKEIYDRLPGLDCGSCGAPTCLAH
                     AEDVYNKESEFEDCVVLRVKK"
     gene            complement(2896..3321)
                     /locus_tag="PKK75_02730"
     CDS             complement(2896..3321)
                     /locus_tag="PKK75_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ82541.1"
                     /translation="MTERILKRYSYNVAANDFMVAGKASSAIKGNLKSLGIDANTIRK
                     AAIVSYETEINIVIHSFGGKLHCDIYENKIVIISEDTGPGIKDVDMALTEGFSTATDS
                     VRELGFGAGMGLPNIKKYSDLLEIKTGDKGTTINVTINL"
     gene            complement(3314..3667)
                     /locus_tag="PKK75_02735"
     CDS             complement(3314..3667)
                     /locus_tag="PKK75_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNZ82542.1"
                     /translation="MIIKDIVDAVKADVLTNYQDSSADIKYGFASDLMSDVLAYGCSD
                     ALLITGLNNPQVIRTAEMLDINVVLFVRGKRPGKEIVELAQENSLTILATDYTMFKTC
                     GLLAIKGMEGLEIDD"
     gene            complement(3679..4545)
                     /locus_tag="PKK75_02740"
     CDS             complement(3679..4545)
                     /locus_tag="PKK75_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNZ82543.1"
                     /translation="MEAAIIKAFTYENKGGNGAGVLVLDEQISNDKKQEIAYKVGLSE
                     TAFIFKLDEKDYDLSFFTPLCEVDLCGHATIAAFYYLGKTGHIKGFNETRTVFQNTRA
                     GRLKIEITFKNEDVEYVLMQQSEPHIYGELKDKLQIAKSLGMDESSIGLPDCDINPTI
                     VSTGLKDILIPVVTRDVLNSIKADFKMIEDISNENDVVGYHVFTIEEGTVYTRNFAPR
                     VGINEECATGTSNGALGSLLYSKGFSNQIEVIQGEAMNQKSLIYVKVDNNNGIINVYV
                     GGKAYIEDYIKI"
     gene            4681..4766
                     /locus_tag="PKK75_02745"
     tRNA            4681..4766
                     /locus_tag="PKK75_02745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4714..4716,aa:Leu,seq:gag)
     gene            complement(5195..5716)
                     /locus_tag="PKK75_02750"
     CDS             complement(5195..5716)
                     /locus_tag="PKK75_02750"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNZ82544.1"
                     /translation="MESENILIRKSIFDDCEIFTRWEQQDYVKEFLTINKERTYEEVV
                     REFILREQDPSNEQYTIIHKNKPIGRIYLSKISKEFDSIDITRIYIGEKEYLGKGYGR
                     ECMELLLDYCFNELKMERVSLDHYTGNAAGELYLKLGFKYEGVMRNSAKKDNEYFDLH
                     LMSMLREEYKKTI"
     gene            complement(5761..6279)
                     /locus_tag="PKK75_02755"
     CDS             complement(5761..6279)
                     /locus_tag="PKK75_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNZ82545.1"
                     /translation="MHLIIALSAHEAAHLFTAILLNIRFTKVRITLFGLNINAHLETV
                     SQAKKIILFFAGPVCNLILYFGFRNTEYLRFANMNLFLAYMNLIPLVPLDGGNICKAI
                     LEVFLDVKAVSRYITMTNAFFITFFIFIIHIYKNYLYFLLVVMGLKGIVEENSYLLEK
                     TVIKAYNKYKMQ"
     gene            complement(6292..6915)
                     /locus_tag="PKK75_02760"
     CDS             complement(6292..6915)
                     /locus_tag="PKK75_02760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ82546.1"
                     /translation="MNKITKKRLSIKRKDQVNKKLALQVIFSIVLVAAVIVTKNINSD
                     LSEKFIEVADKKMSASIELLEVRDTISEGFSAAIDKLPLVGKADAKPVMGTLYRKYGL
                     NKTDEVSYYNHGIDIKADTQSVKALTEGRVISLGNNEKLSGYIIIQNEDKTFIYGKIN
                     EAFVKEGDNISKGDVIGALSEEKMILHIEVWEDGASINPSSLFDLTE"
     gene            complement(7040..7723)
                     /locus_tag="PKK75_02765"
     CDS             complement(7040..7723)
                     /locus_tag="PKK75_02765"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ82547.1"
                     /translation="MNVVLTGSQKNNIGKTIIGIKLSLELARSGKKVLMADLSAGKIK
                     MAEYLNVNEDVIYDSVDVLKKTCTLEQGIIEINENLSLLPSPRAAGKINELDRESFKS
                     LLDSIEGYDYVIIDADKLTSSYVDLSKVNNAITINSNDFSCIREINSDKTISSKAVNF
                     VLVINKYNRKSAKKGLMMKAGDIERLTETPASSIIEENPGYLNMKHEMLLDSSFLKTE
                     IGGIIKNLR"
     gene            complement(7842..11183)
                     /locus_tag="PKK75_02770"
     CDS             complement(7842..11183)
                     /locus_tag="PKK75_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ82548.1"
                     /translation="MIKFFKNRFNILYMIIVLLLIILGFRMAVLTIVEGQDYRQIADI
                     KKIKDIPVKAPRGKIFDRNGLLLADNVSSFTVQMYTDEIKAEDFNDISYKLSKIFDEN
                     MERPIDEFPIELDTFDYIDKNVEIIQSDTDQSEEIETAACQTAEEKVIERVKENIYEW
                     LNFSIFMYGENFSPKQRVYAAIIKDEVPIQLKGENFEYNEELLEPIVNGVSIDGNELP
                     EQKTPIQIWLSENNLRENLSAEEAAAQLLINNNKYLSSLFSNSKIRKLSYEFLKQKGL
                     VENIKLNEFSFVQDEEYENIKRNLNASNEEITLNTSAKDDFVILTMKNSIDNLLRTVY
                     HEPENIKPGAILIDRLKDIYPDLPVKFIEEGETGIFEYTDTETRDRYFNQQHMDYSTT
                     AYVFVKELAFKNYDVLFNLVTDDNIKYFSQIELLKYVNPSISIAEWEYTPLRDKNAWI
                     IKNVSTYDDEMDDYSASKVFKMLRESLEIDEEINDYELRNMMVLRERYNRISYLSYHP
                     VDICYGISEKTVAMISERSHELNGINVEIEPLRYYPQYESASHIIGYLGKIAQDYELQ
                     EYVVEKGYSTDDIIGKTGLEEKFEDYLRGEKGRKTVEVNNVGRTIRSVSSEAPVPGDD
                     IFVTVDLQLQKKAEESLKKGLEKIREGGVFESEWGDFHFKDKYENAYSGALVALDVKT
                     NEVLALANYPAYDLNLFATGITMENWNSLLNDSKNPLAPRPLYNISMLTAIQPGSTFK
                     MITSLAALEKGVNPNTTVYCSGTMKVGDRYFSCWIHNMFGGRHGSQTMYQAIMNSCNF
                     YFWTTVLGENLATHQKHTVKVDAEDIIDMAGKFGLDSKTGIEIDIPQEASGGVPSIEG
                     KKSGMRVYLRLFLEANVERYLNDGFVVDSSMKNEIVEEIVSWIDREEPMTRGEVYEGL
                     LSLNLNPDKTNDNYVPLVDIIKYSFINQATWTVGDNLNISIGQGNNAYTTLQMANYVS
                     SLASGYRRNVSVIKEIKTYDGKKTDYVPLREEEKIPLTDDKYLDVVRQGMKLVSYDDS
                     AKPYASFPVEVGSKTGTAQNQGINPETGKTYDDFAWYVAFAPYDDPQIAVACVLFEGG
                     SGRYPIPIVREVIGEYLRINSTKMP"
     gene            complement(11188..11682)
                     /gene="mreD"
                     /locus_tag="PKK75_02775"
     CDS             complement(11188..11682)
                     /gene="mreD"
                     /locus_tag="PKK75_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNZ82549.1"
                     /translation="MKKFIFMASLIITGFVIQTSLMGFFNFFNVIPNISLILLVIFAM
                     LTDGVSGGTLGLLTGILYDAMIYDIFGLYALIYFLTGAVIGTISDEMLRENQLVYSLA
                     AGLSTIVMHLLLFLILFFLRFRVQSAHGLLPSILVETILNAVLVVFVLRFFIYLSDRF
                     NVKA"
     gene            complement(11700..12533)
                     /gene="mreC"
                     /locus_tag="PKK75_02780"
     CDS             complement(11700..12533)
                     /gene="mreC"
                     /locus_tag="PKK75_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNZ82550.1"
                     /translation="MNFLKRHFKKILFFLTIFILIIMIGLSSIGRMEGADLGFFGNFI
                     AGLQKLTYKTGGTFTNSFYSVQEIVKMREENIMLTENVYKLRDQVRMLENIVNKSDAL
                     KTEYEMRKSLSYDYVVGQVIAVDNSNWFSRFTIDRGENDGIRQNDIVIQAVKTDDNIV
                     QIGLLGIVAETSSDWSKIITVTDERCKVSFKNNANDESGIVQGSIDGTITGYFFNSKA
                     GAKAGDSLVTSGIGEVYIPDIYIGDVKDVVKTTDASTLRIIVEPAVDLTKIGRVYILT
                     K"
     gene            complement(12544..13692)
                     /locus_tag="PKK75_02785"
     CDS             complement(12544..13692)
                     /locus_tag="PKK75_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNZ82551.1"
                     /translation="MGLFDIFTQKIGIDLGTSNTLVYVNGRGITLNEPSVVALNTVSY
                     EVCAVGVDAKAMLERTPKTIRAIRPLQNGVIADFEITRAMIKYFITKALNKKRFFKSN
                     IVISVPVGVTSIERRAVEEVAYDAGARKVKLVEEPMAAAIGAGLMVDQPVGNMVVDIG
                     GGTTEIAVISLGGIVESDSLRIAGDDIDLNIKDYVKSKYKMDIGIVTAENIKKVLGNA
                     SRDYYKRIIEDMNVSKAENIEEDKDKYTETIEVNKDAGKKDKDKKVKEEKEDNNKADA
                     AQMMEIRGRDIVSGLPLKITITSDEIREAILETVNSIVNAIKRVLERTPPELSSDIYT
                     NGIYLSGGGALLKGLDIFIERETGIKVFIADNPLQSVALGAGKICESL"
     gene            complement(13711..14400)
                     /gene="radC"
                     /locus_tag="PKK75_02790"
     CDS             complement(13711..14400)
                     /gene="radC"
                     /locus_tag="PKK75_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNZ82552.1"
                     /translation="MDNTNYTIKSIPVDDRPQEKLLKYGANTLSNSELIAVILRTGTK
                     EENVVMLSQRILKEEGRGLRNIAEGTVEKFKSYKGISDVKAAKLMALAEISKRISTLK
                     IEKVKISSPDDAAVIMMEEMRYYRKEYFKIMLLDTKNNVKKVSEISVGSLNSSIVHPR
                     EVFHEAVSNLASSIILVHNHPSGECEPSHEDVVLTNRLDECGKILGIRVLDHIIIGDG
                     VYFSFKEEGLI"
     gene            complement(14428..15003)
                     /locus_tag="PKK75_02795"
     CDS             complement(14428..15003)
                     /locus_tag="PKK75_02795"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNZ82553.1"
                     /translation="MKKIILASSSPRRIEMLKRYADITIMAPGTKEKANSFELPQTTV
                     MKVAFEKALDIYTKCQEKALIIAADTIVYSDRIMGKPKDETEAYEMLSRLSGKTHCVY
                     TGICIIDSENSKKILDYEKTQVVFNRLEDELIRRYLKTNEYQDKAGAYGIQGYGELLV
                     KEIQGCYNNVKGLPVAKLNNLLSEHFSVNLL"
     gene            complement(15012..15254)
                     /locus_tag="PKK75_02800"
     CDS             complement(15012..15254)
                     /locus_tag="PKK75_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HNZ82554.1"
                     /translation="MRKMSSVYFILLILTSVIIGTILGKAFSTALPILNYGEGIGFGP
                     ATLDLNIFTLTFGFSANLTVSGIIGLIAGIIIYRKL"
     gene            complement(15256..15777)
                     /locus_tag="PKK75_02805"
     CDS             complement(15256..15777)
                     /locus_tag="PKK75_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNZ82555.1"
                     /translation="MNKIQRYIFLSLLTAAALILSIIEGMIPLPYIAPGAKLGLSNIV
                     ILSVVVIFGPKDALLVVIIRSILLMLVATNPITFIYSVTSGIVSTLVMSLGCRYLNNY
                     FSLIGISVLGAMAHNTTQVTVAAMLFTTFNLYYYLPILSLVSIFTGCFVGYTAIFVTD
                     NLEKTLLKLGREA"
     gene            complement(15790..16179)
                     /locus_tag="PKK75_02810"
     CDS             complement(15790..16179)
                     /locus_tag="PKK75_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNZ82556.1"
                     /translation="MKKKINKYDIGLIAAIIIINVFLIIYGGKTAVASNSKTAYIYSD
                     NQLAGQYVLTDGIKDEIRIESKEGYNVVHIEDGRIWIHDASCPDKICISQGKISSDGE
                     IIVCLPNKMFIKIIDENDNDEIDFIAD"
     gene            complement(16330..16962)
                     /locus_tag="PKK75_02815"
     CDS             complement(16330..16962)
                     /locus_tag="PKK75_02815"
                     /inference="COORDINATES: protein motif:HMM:NF025391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HNZ82557.1"
                     /translation="MYINLDVLNITKGNTQIADSEFGRMNIERIEAGRLKLLTGRIIA
                     TDPILLYDDECYSEHVKPGTYRVNVYVGKAEKRKKQTVAAELRINENEPVKWEMALFE
                     GESAKAFSRDEFMGYEVENGLGCFMDEKVMEMLDIMSEEELEAYEIKVKDAVRQSGNS
                     CADIIMDEEKGLNIVVFASGWNEGTFPTYCGFDKSGRLSRLVTDFMVIES"
     gene            complement(16982..17443)
                     /gene="nrdR"
                     /locus_tag="PKK75_02820"
     CDS             complement(16982..17443)
                     /gene="nrdR"
                     /locus_tag="PKK75_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNZ82558.1"
                     /translation="MRCPKCNYTESKVIDTRPTDDGFKIRRRRECIECGHRFTTYEKI
                     EETQIVVIKRDGTRQGYNRDKIINGLIRACDKRSVSLEQIEQIADKVEKQLYNTFQNE
                     VSTELIGELVMNELQGTDDVAYVRFASVYRKFKDIDTFMDELTKILNERKK"
     gene            complement(17521..17901)
                     /locus_tag="PKK75_02825"
     CDS             complement(17521..17901)
                     /locus_tag="PKK75_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNZ82559.1"
                     /translation="MELNLSKGLKYKEEKIVKMEDTARVFGSGAAEVFATPMMIGLME
                     AASMNAVKDYLQEGYSTVGTMVNIKHMSATRIGKKVWAEAELIDIDRKRLVFKVTAYD
                     EDKMIGEGTHERFVINDERFMSKL"
     gene            complement(18205..19596)
                     /locus_tag="PKK75_02830"
     CDS             complement(18205..19596)
                     /locus_tag="PKK75_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82560.1"
                     /translation="MIMQDRENFSNLLVRLKFSAMMALDGITSNVLRTFLTVLGISIG
                     VAAVISLTGIGEGARRSIVAQFESLGENVIVIKSNAENVKFEIEEAETLPDRVSGIEY
                     ATPVLYTEDTPIRWRRDESEMNIVGVNENYPQIKDHPVISGNFFTSLHVKQRSPVVVL
                     GYNVANSLLNGRSPVGQTMKINGINYRIVGVLGEKGEGKGDGIDDKIVIPYTSAMKIT
                     NERDILEIWAKSNSAEDAELAMVQLGRIYKRKVGLGSNIKPDNGQGEQPPGDGGDMPG
                     DMPADMPIEEPMPTEPMPEKPMPEEPGIPGQDENVFEKGEEPLTITSLNNLVKEADQA
                     NRIMTVLLGGIAAVSLLVGGIGIMNIMLVAVTERTSEIGVRRALGAKKEDLLIQFLLE
                     AVYVSIIGCFAGVLLGAWAVSIVARYGLSAVVSFESVQIAVFVALVTGLLFGVYPAIN
                     ASNLPPVEALKRQ"
     gene            complement(19611..21329)
                     /locus_tag="PKK75_02835"
     CDS             complement(19611..21329)
                     /locus_tag="PKK75_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ82561.1"
                     /translation="MYKKFISIGIVIALVFLGGYLTAKWMIPDDSEASAGPRYSTKAV
                     ERGDIKQGVSISGQLSGNWGGSITAPKPEGIGEVTYTVEEVFVEPNQMIKKGDNLIRL
                     SASNLGDILTGFTDQIQAKQEDIQGKMSNLEKILNQDISNINQVNAYDGIVFRAPIRG
                     RITELNVEEGEKVDNFNIATIVDDSMVKIPFKVNTYEYGNLKEGQKVLLQYRRTTEHG
                     SQLAFEGFYPGTITKLNKNKVPNGMTYVHNGTIEADNPGLVQPGMTVTIYTENNGNPV
                     TAFSYPGEVESFVNEKKITYSYIPGGDSSNVIASEVFVSTNEFVEEGDLLVRIAGSDV
                     TQIIQQEVDAISNAYDEINSIYKKIDKVSELTGKMMVTAPTDGMVAYVNYRQGDQIMA
                     NENSDQWALMLMDVYNTDEMFIYTTVSDLDVMYVAQDAPVTVTVDALPGETFQGKVMR
                     LDSYTDYGTGKTIYNVQIQVEGREGLRPGMNTNCFVDSGESLDTLLVPIEAVFEEDGK
                     QKVEILTGENQVEIVEIETGLMNDMYVEVLSGLEEGQLVVTGSTKDLMPSQSIPGNDS
                     ILPGTI"
     gene            complement(21322..22020)
                     /locus_tag="PKK75_02840"
     CDS             complement(21322..22020)
                     /locus_tag="PKK75_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82562.1"
                     /translation="MDIIKMENISKLYKSGQIQVEALKNISFNIKEKEFVSIMGPSGS
                     GKSTLLNLIGCLDHPTGGTYELAGTEVNKLSDSKMSETRNLFIGFVFQSFHLLPRMNA
                     LRNVEIPLIYRGMNHKKRKELAENALEQVGLKDRMTHLPGQLSGGQQQRVAIARAIAG
                     NPSIILADEPTGALDSKSGEMVMELFIELNEKSSMTVVQVTHEEFIAEYGSRIIRLVD
                     GQIVSDEVVEGKNV"
     assembly_gap    22257..22356
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22398..23798)
                     /locus_tag="PKK75_02845"
     CDS             complement(22398..23798)
                     /locus_tag="PKK75_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82563.1"
                     /translation="MNNNYTIIRTIYENVFQRILECKDNDTGEVFYSNIITSKKVINL
                     INLQDLKKLNSNIIECYNTEDRIYIFTKSLEADCIKLTDLASTDLSMKQQFNLAEKSI
                     NLASSIYNMTDVVQQKILDLKRLYIDDNNELIVDCNLIFEQEYDIADNETFKRLGSII
                     HMIFSGTEIVDYNISDSIPPDILKIIVRCLTREYVFPNDALIELRRSPIFIMIFGEEI
                     GIEKENEKPENPSIIIHDESSEDILNLYLGDEKPPKKKISLSKEIKRAALSLFIVVMV
                     LLSGNAIINGLNKNKDGSTETINPGKPPAQETPSQPGEEKPPLESTDIHFSEELLDSI
                     GYEGAAAQRDTEIYVEGNSSLLVVNDGQDKVKALFAAVDFTDADVSYMLMKQIGVSGK
                     IKSQYDTQVQVVFEAYKDGVMKSNFHSLVNSYDDIWSQFTVPINVTDADILCVYLEYE
                     GENKVWIDSLLIDVIK"
     gene            complement(23893..24174)
                     /locus_tag="PKK75_02850"
     CDS             complement(23893..24174)
                     /locus_tag="PKK75_02850"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNZ82564.1"
                     /translation="MGRVLFILKFLFDKNVPLREKWWVIIPVIYILSPVDLIPAPVLG
                     LGIVDDLTMLGFLLTIIYEKTKKYYDDNKNEGKDIIEDVEYKIHEDEEK"
     gene            complement(24184..24552)
                     /locus_tag="PKK75_02855"
     CDS             complement(24184..24552)
                     /locus_tag="PKK75_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNZ82565.1"
                     /translation="MPNCVLVCVTRQKTSEKLIKKGVELTKGEADSCLNVIHVVNEND
                     KLLYGLSDGDALEYLFEITKDVGAANLVVKRSKDVIKTIVSYAEEINCTHIVVGHSNN
                     SSNSFISKLQKKLPNVDFII"
     gene            complement(24570..24664)
                     /locus_tag="PKK75_02860"
     tRNA            complement(24570..24664)
                     /locus_tag="PKK75_02860"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24628..24630),aa:Sec,seq:tca)
     gene            complement(24853..25539)
                     /locus_tag="PKK75_02865"
     CDS             complement(24853..25539)
                     /locus_tag="PKK75_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ82566.1"
                     /translation="MRVLIVDDELLTIKNLKYSFEQDNFEVESTDDSNDALKRIITNE
                     YDAIILDLILPETDGLEVCQRIREISNVPILILSANNEDMTKILALEYGADDYISKPF
                     NILELKARIKAILRRTRNSSQKANEQTLVVDDFTINALGRKVSLHNQEINLTAKEFDL
                     LLLLITNPGKVFSREELLEIIWGYEYFGDLRTVDVHVRRLREKIENKNKAYEYILTKW
                     GVGYYFRTKN"
     gene            complement(25626..>26053)
                     /locus_tag="PKK75_02870"
     CDS             complement(25626..>26053)
                     /locus_tag="PKK75_02870"
                     /inference="COORDINATES: protein motif:HMM:NF020672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HNZ82567.1"
                     /translation="IKQTDEFISLPYFTVEYDEDYKRIKEKALNILNDVKFEFLKFEE
                     LDEKIGLPMTNDVLSLMMAEKDLVKINELVTSKILYEEAKNILVEFLKKNKKISAAQY
                     RDLLNTNRKTAITLLEHFDMVKLTKRVENDRILLNFTIE"
BASE COUNT         7235 a         5525 c         3922 g         9271 t
ORIGIN      
        1 ttatcatttt taatcaaata aagcaataat aatgaaaagt aatatttacc tgtttaaaac
       61 aatatttaca ttttcattaa tatattcctt tatccacaat aaaatgctta tgtcattaat
      121 gtcaatgtca tcaagcattt cttttacttc tctcgtatca aaagtaaatt caccggaatc
      181 ggtcttatgg ttataaagca gttctatatt atcagcggag ttaataatag tcattattgt
      241 ttcgcccata ttcccaagag gtgccctgtc tatgttatct ctttcaaaag aacaaaaaac
      301 cgtggttcct aaacctacct ttgattctat tttaagatac ccgctgcatc tttcgcaggc
      361 ttctttcagc attggaatac ccaacccgac cttcctggtt gtcctgcttg tgtaaaaagg
      421 atttgttact ttgtttataa cttcctcgct cattccattt ccgttatctg aaatagctat
      481 tgtaagattg ttgtttttct tgctgtcttc aatttttatt tcaaccaatg aggcttcagc
      541 ctttatgctg ttcattgtaa tatccagtat gtgcattgaa agttctttca tagttcctcc
      601 atgtacaggg ttcgcatacc taaaaattca taaacatcat ctatattatt aagatcaaaa
      661 taattaattg cttcagatat gtcgaccagc cgatgagcgt cagaattata aagaaccttg
      721 tacttttcaa ttgttttatc gctaataggc acttttgaat atacttccac atgctcaatt
      781 ttcagattaa agggcataaa acctaaaact cccagaatgc cgttggtcat tttattaata
      841 tgggcaggaa tcataactcc cctgtattct tttacaatgg agtatatatc ttcaatagta
      901 tatttagagg cattgatcaa cagtttttcc agagttcctg ttatttcatc cttagaatta
      961 taaatgttct gctctccgaa tatttctata ttgtttttta tcaaggggag cgaatcgtag
     1021 ataatagagc caaattccgt tgcatttttt agttccttaa aataacatag aacatggact
     1081 tcttcttttg atgtaacctc aagccccggt atgactttaa tacctgcctt gccgcttaac
     1141 tccattgctg caggcaggtt ggccgctgaa ttatggtctg tgatcgatat gatattcaaa
     1201 ccttttatca aagacatatt tataatatta ttcggggtca tgtcgcttga gctgcacgga
     1261 gataaatccg aatgaatatg caaatcgtag taatatttca tcttattccc agttctgaca
     1321 ttctgcatgc catttggtat gcactttccg aagttttaaa aacgggtata cctaattcct
     1381 ttgccttttc aatggtatca ttgtccacat ccatgtcttc aacaactata atgcaggcaa
     1441 gctctataag ctcagcaaca gccactatgt taatatgggt ttggatggta atccatacat
     1501 agtttttctt tgcctttgac attacaatac ttagcagatc tccgatataa acaccttcaa
     1561 tttcaatgtt tccctctttt tcactcaagg gtgtaagctg tagtttttct gcaaattcta
     1621 agcagttcat tttttccctc ctgtttattt tttaactctt aatacaacgc agtcttcaaa
     1681 ttcagactct ttattatata catcttcagc atgggccaaa catgtgggcg cgccacagga
     1741 accgcagtcc agtccgggaa gcctgtcgta tatttctttt attctttcat ccttttcaac
     1801 agcttccttt aaattcagcg ttttggaact ttcttcttcc ttcagataat aattgaagaa
     1861 acctgattca tacatttcat caaatctgtc aatatcagga tttatgcatt catctcccag
     1921 actgtttgat atataattaa tgctgctggt tgcaataaat ggatttacca cattaaatgt
     1981 ccctcctaca caaccgttga cgcaggctga ggcttcaaca aagtctatgt cgcttaattt
     2041 gccgttttca atttcctcca ttatctcgat gatattttcc atgccgtgaa cagctatata
     2101 attattcaaa cctgcatttt ctgactggcc ccccgccact gcccatttca ttcctgaaag
     2161 agaggggtat gagcatgtta tattatcttc ctttataacc tctctgtaga gctctccgta
     2221 tattttgtta agaggaataa cccaatctac cgaaggcttg taatcaccaa taggatttgt
     2281 aactgcaagc agcttggcag gacagggtga aatataaaat acctgtatat cttcatcttt
     2341 cagattgaat ttttttcttg ctctttctct tgaaagtata cctgcaattt ccataggttc
     2401 tcttaataaa attaaattat ccatcaatga aggatatctg tattttataa gtcttactgc
     2461 cgccggacaa tatgttgata tataaggtct cacagttttt tctttctcta ttttaagctt
     2521 tattgctctt gcaaccaatt ctgcagacca gctggcatca tagacaaagt caaatcctaa
     2581 ttttaaaatt gcattttgta ccttgcatat atctgttcct ctgggaaact gcccgtataa
     2641 tgatgtggta ggaattgcaa ttttgtattt ttccggtgta tcatgcattt gatcaaccca
     2701 ttcaattgat tcaggtgtat atgcattgta agggcagatt tttatacatt cgccgcagtg
     2761 tatacattta gatatattta taaatgcttt gttgttgatt agtctgatag cctgtgtagg
     2821 gcatcttttc atgcagtttg tacatccttt gcatttattc tcgtcaaaca taattgaaaa
     2881 attcatattt tcctcctata aattgattgt cacatttatt gttgttcctt tatcccctgt
     2941 ctttatttcc aataggtcgg aatatttctt aatgttagga agccccatgc ctgcaccaaa
     3001 acccagctct cttacactgt cagttgcagt tgagaatccc tctgtcagtg ccatatctac
     3061 atccttgatg cccgggcctg tatcttccga aattattaca attttatttt catatatatc
     3121 acagtgaagt tttcccccaa aggaatgtat cacaatattt atttcagttt catatgatac
     3181 aattgctgct tttcttatgg tattggcatc tatgcccaat gatttcaaat tgccttttat
     3241 ggcacttgaa gcctttccgg caaccataaa atcatttgca gccacattgt agctatatct
     3301 ctttaaaatt ctttcagtca tctatttcta gtccttccat tcctttaata gccaataaac
     3361 cgcatgtttt aaacatggta tagtcagttg ccagtattgt aagtgaattt tcctgtgcca
     3421 attctacgat ttccttgccg ggtcttttac cccttacaaa caatactaca ttaatatcaa
     3481 gcatttcagc agttcttata acctgaggat tgttcaaccc cgttataagc aatgcatcac
     3541 tgcagccata agcaagcaca tcactcatta aatcactggc aaaaccgtat tttatatctg
     3601 cgctggagtc ttggtagttt gtaagaacat cagcttttac ggcatcaaca atatctttta
     3661 ttatcatact atcccccgct atatttttat ataatcttca atataggcct taccgcctac
     3721 ataaacattt attattccat tattattatc aacttttaca tatatgaggc ttttttggtt
     3781 cattgcttct ccttgaatta cttcaatttg gtttgagaaa cctttactgt agagaagact
     3841 tcccaaggca ccgttagatg tacccgtggc acattcttca tttatgccaa ccctgggagc
     3901 gaagtttctt gtatatactg tcccttcctc aattgtgaaa acatgatagc ccactacatc
     3961 attttcgtta gaaatatcct ctatcatctt aaagtcagct ttaatactat taagcacatc
     4021 ccttgtgaca accggtatta aaatatcctt taggcctgtt gacactattg tggggttaat
     4081 atcacaatcc ggcagtccta ttgagctttc atccatacca agtgattttg ctatttgcag
     4141 cttgtctttt agctcaccgt atatgtgtgg ttcgctttgc tgcataagaa catactccac
     4201 atcttcattc ttaaatgtta tttcaatttt aagcctgcct gccctggtat tttgaaaaac
     4261 cgtccttgtt tcattaaagc cttttatatg accagtttta cccaagtagt aaaaggcagc
     4321 aattgttgca tggccgcaaa ggtcaacctc acaaagggga gtaaagaaac ttaaatcata
     4381 atccttttca tccaatttaa aaataaaggc tgtttctgac aatcccactt tatatgcaat
     4441 ttcctgcttc ttatcatttg atatttgttc atcaagaaca agaactcctg ctccgttgcc
     4501 gcctttgttt tcataggtaa atgccttaat tattgcagct tccatcctac ccctcacctc
     4561 tttgtcctat aatcattata gtttaaaatc agtataatta caagtgaaaa tagattatca
     4621 atctttctaa ataaaaccac tttacaaaaa ttattttatc atttataata agcaggtaat
     4681 gcggttgtgg cggaacggca gacgcactag cttgaggggc tagcgaatgt atattcgtag
     4741 gggttcaaat cccctcaacc gcaccatatt tgtttcgggt attacccgaa actatttttt
     4801 tgcttcccca ttatctgttt tatgtattct atttattagt cttaacatat aatatacggg
     4861 gggattgtat gtttttaaga atttggaggt ttttaaaaag atatatattt gttattattt
     4921 ttatattttg catattgact gttttgtttt ctcttgtctt taggtaatgc acagcagggg
     4981 aatgcagctc tttgtttgcc ccttgtgtcg cactctttgg cattccttgc gtttgacctg
     5041 ctataaattt ttaagaaaaa aactatgaaa aatcggcttg ccccacaaag ggcaagccgt
     5101 gaatatcttc acggcttgag gcaaaaaatt taagaggcat ttataaattt ccatttacaa
     5161 atgtattttc attatactac tattttaaaa aaattcaaat ggttttttta tattcttccc
     5221 ttaacattga cattaggtgc aaatcaaagt attcattatc ttttttagca gagtttctca
     5281 ttaccccttc atatttgaac cctagtttta aatacagctc tccggcggca tttcctgtat
     5341 aatggtccaa tgataccctt tccattttca gctcattgaa acaataatca agcaaaagct
     5401 ccatgcattc tcttccgtag cccttcccca aatactcctt ttcgcctata tatatccggg
     5461 ttatatctat tgaatcaaat tcttttgaaa ttttcgacag ataaattctt cctatgggtt
     5521 tgtttttatg gataattgta tattgctcgt ttgagggatc ctgctctctt aaaataaatt
     5581 ctcttacaac ttcctcgtat gtccgctctt tgtttatggt caaaaattcc tttacataat
     5641 cctgctgctc ccatctagta aatatttcac aatcatcaaa aatacttttt ctgattaaaa
     5701 tattttcaga ttccattttt cctccgtaga tttgaaattt tgcagtaaaa acgataggtt
     5761 ttattgcatc ttgtatttat tatacgcttt tatgacggtt ttttcaagca aatagctgtt
     5821 ttcctccact attcccttga gtcccataac aacgagcaaa aaatacaaat agttcttata
     5881 tatatgtatt ataaatataa agaaagttat aaaaaaggca ttggtcatgg taatgtatct
     5941 cgaaacagcc ttaacatcca aaaaaacctc taaaatagct ttgcaaatat ttcctccgtc
     6001 taaaggcaca agaggaatca aattcatgta tgccaggaac aaattcatat ttgcaaacct
     6061 taaatattcc gtattcctaa acccaaaata cagaattaaa ttgcatactg ggcctgcgaa
     6121 aaacaaaatg atttttttag cttgtgatac agtttccaaa tgtgcattta tatttagccc
     6181 gaacaaagtg attcttacct ttgtaaacct tatattcagt aaaattgcag taaacaaatg
     6241 ggcagcttca tgggctgaca gtgcaatgat taaatgcatt tgccaaaagt tttattctgt
     6301 caagtcaaat agtgatgaag gatttattga agctccatcc tcccaaactt ctatgtgaag
     6361 aatcattttt tcttcgctaa gggctcctat tacatcccct ttagatatat tgtcgccctc
     6421 tttaacaaaa gcctcattta ttttcccgta aataaatgtc ttatcttcat tttgtattat
     6481 tatatagcct gaaagctttt cattattgcc caatgatatt actcttcctt ccgtaagagc
     6541 cttaacagac tgggtatcgg ccttaatgtc tatcccgtga ttgtaatatg atacctcatc
     6601 agtcttattc agtccatatt tcctgtagag ggtccccatg acaggttttg catccgcctt
     6661 gcctacaaga ggcaatttat caattgcagc ggaaaagccc tcactaatgg tatcccttac
     6721 ttccaataat tcaatgcttg cactcatttt tttatctgct acttctatga acttttcaga
     6781 taaatccgag tttatatttt ttgttactat aacggccgca accagtacta tgctgaaaat
     6841 gacctgcaac gccagtttct tgttaacttg atcctttctt ttgatgctga gccttttttt
     6901 tgttatttta ttcatttcac caccccttcc aatacatttt attaatgtgt tacaagcttt
     6961 attacaaaat aaaaaaactt ttgtccatgt ttaatcaact gacaaaagtt tgcttacgca
     7021 agaaaggatt tacttgtttt tatctcaggt tcttaattat gccgcctatt tcggttttca
     7081 gaaaactgct atccaaaagc atttcatgct tcatattcaa atatccgggg ttttcttcta
     7141 ttattgatga agccggtgtt tctgtcagtc tttcaatatc gcctgctttc atcataagac
     7201 cttttttagc gcttttcctg ttatacttat tgattacaag aacaaaatta acggcctttg
     7261 aagaaatggt tttatctgag tttatttctc ttatacagga aaaatcatta ctgtttattg
     7321 ttattgcatt attgaccttt gaaagatcaa cataggatga tgttaattta tctgcatcaa
     7381 ttataacata gtcgtagccc tctatgctgt caagcaggct tttgaatgat tccctgtcaa
     7441 gctcatttat tttgccggct gccctggggc tcggtaaaag tgataaattt tcattaattt
     7501 caataatgcc ttgttccagg gtgcatgttt ttttcaaaac atcaaccgag tcataaataa
     7561 catcctcgtt cacattcaaa tattcagcca tcttgatttt tcctgctgat aaatccgcca
     7621 taagaacctt tttccctgac cttgcaagct caagagacag ttttatgcct attattgttt
     7681 tacctatgtt atttttttgt gaacctgtta atacaacatt catctttgat ctcctctatt
     7741 tccacattgc taactataag aaaaatgata aaaacatgag tgaacatttg aagtacacga
     7801 ctataactct tagcgataaa ggctaaaaat cagttaaaat gttagggcat ctttgtacta
     7861 tttattctca ggtactcgcc tataacctct ctgactatgg gaattggata acgtcccgat
     7921 cctccttcaa aaaggacgca tgccactgct atctgaggat catcgtaagg ggcaaatgca
     7981 acataccagg cgaaatcatc gtaggttttt cctgtttcag gattaatgcc ttgattttgt
     8041 gctgtacccg tcttgctgcc tacttcaacc ggaaaggaag catatggttt tgcactatcg
     8101 tcatatgaaa caagtttcat accttgtctt acaacatcca aatatttgtc atcggtcagg
     8161 ggaattttct cttcttctct caatggaaca tagtctgttt tctttccgtc ataagttttt
     8221 atttccttta tcacgcttac atttctcctg taaccgctgg ctagtgagga tacgtaatta
     8281 gccatctgca aggttgtata ggcattattt ccctggccta tgcttatgtt taaattatca
     8341 cccactgtcc aggttgcctg gtttataaat gaatatttaa ttatatccac caggggtaca
     8401 tagttgtcgt ttgttttgtc agggtttaag tttaaggata aaagcccttc ataaacttca
     8461 ccccttgtca ttggctcttc tctgtctatc cagcttacaa tttcctcaac aatttcgttt
     8521 ttcatcgatg agtcgacaac aaagccgtcg ttaaggtatc tttccacatt tgcctcaaga
     8581 aataaccgta aataaactcg cattccggat tttttccctt caatgcttgg tacacctcct
     8641 gaagcttcct ggggtatgtc tatttcaatt cctgttttgg aatctaatcc aaacttgcct
     8701 gccatatcta ttatatcctc tgcgtctacc ttcactgtat gcttttggtg ggtagccaga
     8761 ttttctccga gcactgttgt ccaaaagtaa aagttacagg aattcattat tgcctgatac
     8821 attgtctgag acccgtgcct tcctccaaac atattgtgaa tccagcagct gaagtatctg
     8881 tcccctacct tcattgttcc tgaacaatac actgtggtat ttggattaac ccccttttcc
     8941 agcgcagcca aagaggtaat cattttaaat gtagaacccg gctgtattgc cgtcaacatt
     9001 gatatgttgt aaagaggcct tggagctaaa ggattcttgg aatcattcaa aaggctgttc
     9061 cagttctcca tggtaatccc cgttgcaaac aaattaaggt cataggcagg ataatttgcc
     9121 agtgctaaaa cttcatttgt ttttacatca agtgcaacca aggctccaga ataggcattt
     9181 tcatatttgt ccttaaaatg aaaatctccc cattcgcttt caaatacacc gccttcccga
     9241 atcttttcaa gccctttctt taaggattct tcagcctttt tttgaagctg aagatccaca
     9301 gttacaaata tgtcatctcc cggaacaggt gcttcgcttg acactgaccg tatagttctt
     9361 cctacattgt ttacttcaac tgtttttctt cccttttctc ctcttaaata atcttcaaat
     9421 ttttcttcaa gcccggtctt gcctattata tcgtcagtag agtaaccctt ttcaacaaca
     9481 tattcctgta attcataatc ctgggcgatt ttacccagat aacctatgat atgtgacgca
     9541 ctttcatact ggggataata cctgagtggt tcaatttcaa catttatccc gtttagttcg
     9601 tgagaccttt cagaaatcat tgcaactgtt ttttccgaaa tcccgtagca aatgtccacc
     9661 ggatggtatg aaaggtagct tatcctgtta tacctttctc ttaataccat catgttccgc
     9721 aattcatagt catttatttc ttcatctatt tccaaagatt ctctaagcat tttaaaaact
     9781 ttcgatgcag agtaatcatc catttcatca tcataagtac ttacattttt tataatccat
     9841 gcatttttat cccgcaaagg tgtgtattcc cattcagcta ttgatattga agggtttaca
     9901 tattttaata gctctatttg tgaaaaatat tttatattgt catctgtaac caaattgaat
     9961 aagacatcat agtttttaaa ggctaattct tttacaaaaa cataggctgt tgtgctgtaa
    10021 tccatatgct gctggttgaa atatctatct ctggtttctg tatccgtata ctcaaatatg
    10081 cccgtttcgc cctcttcaat aaatttaacc ggcaggtcgg gataaatatc tttcagcctg
    10141 tctatgagaa ttgctcccgg ttttatattt tcgggttcat ggtatacggt cctgagtaaa
    10201 ttatcaatgg agtttttcat tgttaatata acaaaatcat cctttgcaga tgtatttaat
    10261 gttatttctt cattggaagc attcagattt ctttttatat tttcatattc ttcatcctgc
    10321 acgaaggaaa attcatttag cttgatattt tctaccaatc ctttttgttt tagaaattca
    10381 taggaaagct ttcttatttt tgaattggaa aacagacttg ataaatattt attattattg
    10441 attaaaagct gggctgctgc ctcttcagca cttaaatttt cccttaaatt gttttcggaa
    10501 agccagattt gtatgggtgt tttttgctcc ggcaattcat ttccatctat agaaacgcca
    10561 ttaacaattg gctcaagcaa ttcttcattg tattcaaaat tttcaccctt taattgtatc
    10621 ggaacttcat cctttatgat tgccgcataa accctttgtt tgggagaaaa attttcacca
    10681 tacataaaaa tactgaaatt cagccactca taaatgtttt ctttcaccct ttcaattact
    10741 ttttcttcag cggtctgaca ggcagctgtt tcaatttctt ccgactggtc agtatcgctt
    10801 tgaataattt ccacattttt atcaatgtaa tcaaaagtat caagttcaat ggggaattca
    10861 tctatgggtc tttccatatt ttcatcaaat attttagaca atttatatga tatatcatta
    10921 aaatcttcag cctttatttc atcagtgtac atctgcacag taaagcttga cacattgtcg
    10981 gcaagaagca agccgtttct gtcaaagatt tttcccctgg gagcttttac aggaatgtcc
    11041 ttaattttct ttatatcggc aatttgtctg tagtcctgtc cttccacaat ggttaaaacc
    11101 gccatcctga aacctaaaat tatcagcagc aggactataa tcatgtataa aatattaaaa
    11161 cgatttttga aaaactttat cattctatca tgcctttaca ttaaatctat cagataaata
    11221 tataaaaaat cttagtacaa ataccacaag cacagcattt aatattgttt caacaagtat
    11281 agaaggcaat aagccatgtg cggactggac cctgaatctt aaaaagaata aaataagaaa
    11341 gagcaataaa tgcattacta tggttgaaag gccggcggca agactgtaaa caagctggtt
    11401 ttccctcagc atttcatcgc ttatggttcc tattactgct cctgtcaaaa aataaattaa
    11461 agcatataaa ccaaagatat cataaatcat tgcatcgtat aaaatcccgg tcaaaagacc
    11521 cagggtccct ccgcttactc catctgtcag cattgcaaaa atcaccagca atatcaggga
    11581 aatatttgga ataacattga aaaaattaaa aaatcccatt aatgatgttt gtataacaaa
    11641 tcctgtaatt atcaaagagg ccataaaaat gaactttttc atatttaaac ccttaatcat
    11701 tatttcgtta gaatgtacac tctgcctatc tttgttaaat caacggcagg ctcaactata
    11761 attcttaagg tggaggcatc ggttgttttg acaacatctt taacatcacc tatataaata
    11821 tcaggaatat agacttcgcc gattcccgat gtaacaagac tgtctcctgc ctttgcacct
    11881 gccttgctgt tgaaaaaata acctgttata gttccgtcaa tactgccctg aacaattccg
    11941 ctttcatcgt ttgcattatt tttgaaactt accttgcatc tttcatctgt gacagtaatg
    12001 atcttggacc agtctgaaga agtttctgca acaattccaa gaagacctat ctgtactatg
    12061 ttatcatctg ttttcacagc ttgtataact atgtcgtttt gccggatacc gtcattttct
    12121 cccctgtcta tggtaaacct gctgaaccag ttggaattat caacagcaat tacttgtcca
    12181 acaacataat cataagaaag gctcttcctc atttcatatt ccgttttcag agcatcggat
    12241 ttattgacga tattttccag catccttacc tggtctctga gtttatagac attctcggta
    12301 agcatgatgt tttcttcacg cattttcaca atttcctgta cggaatagaa tgaatttgta
    12361 aatgtaccac ctgtcttgta ggtaagcttt tgcagacctg caataaagtt tccaaaaaag
    12421 cccaggtcgg ctccttccat ccttccgatt gatgacaatc ctatcattat tatcaatatg
    12481 aaaattgtaa gaaaaaataa tattttctta aaatgccttt ttaaaaaatt catttatcca
    12541 ccgctataaa ctttcacaga ttttgcctgc acccaaagct acgctctgca atggattatc
    12601 ggcaataaac acttttattc ctgtttctct ttcaataaat atatccaaac cttttaaaag
    12661 tgcccctccc ccgcttaagt agattccgtt ggtgtaaata tctgatgata attcgggagg
    12721 tgttctttca agaacccttt ttatggcatt aacaattgaa tttaccgttt cgagaattgc
    12781 ttctcttatt tcatcacttg ttatggttat cttcaaggga agccccgaca caatgtccct
    12841 gccccttatt tccatcatct gagcggcgtc tgctttgtta ttatcttctt tttcttcttt
    12901 tactttttta tctttatctt ttttacccgc atctttatta acttcaattg tttcggtgta
    12961 cttatcttta tcctcttcta tgttttcggc cttacttaca ttcatgtctt caattattct
    13021 tttgtaatag tccctgcttg catttccaag aaccttctta atattctcgg cagtaacaat
    13081 acctatgtcc attttatatt tacttttgac atagtctttt atatttaaat caatatcatc
    13141 tccggcaatc cttaatgagt cgctttcaac aattcctccc agtgatatta ctgcaatttc
    13201 cgtagtccct cctcctatat caacaaccat gttccccaca ggctggtcta ccatgagccc
    13261 tgctcctatt gcagctgcca taggctcttc cacaagctta accttccttg cgcctgcatc
    13321 atatgccact tcttccactg ctcttctttc aattgaagta acgcccactg gaacacttat
    13381 cacgatgttt gatttaaaaa atcttttttt attgagggct tttgttataa aatactttat
    13441 cattgccctg gttatttcaa aatccgcaat aacgccgttt tgtaaaggtc ttatggccct
    13501 tatggtcttg ggagttcttt caagcattgc ttttgcatca acacctacgg cacatacttc
    13561 atatgatact gtatttaagg caaccacgga gggttcattt aaagttatac ccctgccgtt
    13621 tacatatact aatgtgtttg atgttcctaa atcaattccg attttctgag taaaaatatc
    13681 aaatagtccc atttatgtat cctctcattt ctaaattaaa ccttcttcct taaaactgaa
    13741 atatacgccg tcaccgatta ttatgtggtc taaaaccctg atgcctaaaa tcttgccgca
    13801 ttcatccagt ctgtttgtca aaacaacatc ctcatgactg ggctcgcatt ctccgctggg
    13861 atggttgtga acaagtatta ttgatgaagc caggttggac acagcttcat gaaacacttc
    13921 cctggggtgg acaattgaac tgtttaaact ccctacggat atttcagaaa ccttttttac
    13981 attgttttta gtatccaaaa gcattatttt aaaatattct ttccggtaat acctcatttc
    14041 ttccatcatt atcacggctg catcatcggg agaggaaatc tttacctttt cgatttttaa
    14101 tgttgaaatt cttttgctta tttctgcaag agccataagc tttgcagctt ttacatcact
    14161 gattcccttg taggatttaa atttttcaac agttccttct gcaatatttc gaagtcccct
    14221 gccttcttct tttaatatcc tctgggaaag cataacgaca ttttcttcct tggttcctgt
    14281 ccttaaaatc actgcaataa gctctgagtt agacaatgta ttagctccat atttcaaaag
    14341 cttttcctga ggcctgtcgt ccacaggtat tgattttatg gtataattag tattgtccat
    14401 tttatccccc cctatgcaat tatttgttta cagcaggttt acagaaaaat gttcagataa
    14461 taaattgtta agctttgcga ccggcagccc ctttacattg ttatagcagc cttgtatttc
    14521 ttttacaagt aattctccgt atccctgaat gccgtaggct cctgctttgt cttgatactc
    14581 attggtttta agataccttc ttataagctc atcctcaagg cggttaaaca caacctgagt
    14641 tttttcataa tccagaattt tctttgagtt ttcagaatca attatgcaaa tacccgtata
    14701 aacacagtga gttttgccag aaagacggct gagcatttca taagcttcag tttcatcctt
    14761 cggtttaccc ataattctgt ctgaatatac aatagtatct gctgctatta tcaatgcttt
    14821 ttcttggcat tttgtatata tgtccagtgc cttttcaaaa gcaaccttca ttaccgttgt
    14881 ttgaggaagc tcgaatgaat ttgctttttc ctttgtacct ggtgccataa ttgttatgtc
    14941 agcatacctt ttcaacattt caattcttct tggtgagctt gatgccaata taattttctt
    15001 catgttcatc cttaaagttt tctgtaaatt attatccctg caataagtcc gattattccc
    15061 gaaacagtca ggttggcact gaagccgaat gtaagagtaa aaatattcaa atccaaagtt
    15121 gccgggccaa agccgattcc ttccccgtaa ttaagtatag gaagcgcagt tgaaaatgcc
    15181 ttgcctaaaa tagttcctat aatgacagat gtcagtataa gcaaaataaa ataaacagaa
    15241 ctcattttcc tcattttaag cctctcttcc aagttttaac aaggtttttt ccaaattgtc
    15301 agttacaaaa atagcagtgt atcctacaaa gcatcctgta aaaatactta ccaatgacaa
    15361 tatgggcaga tagtagtaca gattaaaagt agtaaacagc attgcagcca ctgttacctg
    15421 ggttgtattg tgggccattg ctcccaaaac gcttatgcca atcaggctga aataattatt
    15481 taaatacctg cagcctaatg acattacaag ggtgcttaca attccgcttg tcacactgta
    15541 aataaaagta atgggatttg tagcaacaag cattaaaaga atacttctta ttatcacaac
    15601 aagcaaagca tccttggggc cgaatataac aactaccgac aatattacaa tatttgacaa
    15661 gcccagtttt gctccgggtg caatgtaggg caatggtatc atgccttcta ttatgcttag
    15721 tatcagcgct gctgcagtca aaagcgacaa aaatatatat ctttgtattt tattcatagt
    15781 attctctctc taatcagcaa tgaaatcaat ttcatcgtta tcattttcgt caataatttt
    15841 tataaacatt ttgtttggaa ggcacacaat tatttcaccg tcactggata ttttgccctg
    15901 ggatatgcag attttatcgg gacagctggc atcgtgtatc cagattcgtc catcttcaat
    15961 atgaactaca ttataacctt ccttagattc aattctaatt tcatccttta tgccgtctgt
    16021 taaaacatat tgtccggcca gctggttgtc agaataaata tatgcggttt tactgttgct
    16081 tgcaacggca gttttaccac cgtatataat taagaaaaca tttattataa ttatggccgc
    16141 aataagtcct atatcatatt tgtttatttt ctttttcatt tgatccctaa tcctgtcgta
    16201 atagatttta ttttatcacc attcaggcaa taatacaatg ttttaagaaa gttttaataa
    16261 ttattattac cgggcattcc aggtacatgg acattcctta tcccgctttt gaaggtatgt
    16321 ccccattcct cagctttcta taaccataaa gtcggttacc agtctcgaca gtctgccgct
    16381 cttgtcgaat ccgcaatagg taggaaatgt tccttcattc cagccgctgg caaagacgac
    16441 tatgttcagt cctttttctt catccataat aatatctgca caggaatttc cgctttgtct
    16501 taccgcatcc tttactttta tttcataggc ttctaattct tcttcgctca ttatatcgag
    16561 catttccatt actttttcat ccatgaagca tcccaagccg ttttcaactt catatcccat
    16621 aaattcatcc cggctgaagg cttttgcgga ttcaccttca aataaagcca tttcccattt
    16681 aactggctca ttttcattaa ttctaagctc tgcagccaca gtttgttttt ttcttttttc
    16741 agctttgcct acatatacat ttactctata tgttcccggt ttgacatgct ctgaataaca
    16801 ttcatcatca tacaatagta tgggatctgt tgcaattatt ctgccggtca gcagctttag
    16861 cctgcccgct tcgattcttt caatattcat cctgccaaat tccgaatcgg ctatttgtgt
    16921 attacccttt gtaatattta gaacatctaa gttaatatac atgccagcac cttcttactt
    16981 cttacttttt tctttcattt agtattttcg tcaattcatc cataaatgtg tcaatatctt
    17041 taaatttcct gtacactgag gcaaatctta catatgccac gtcatcagtg ccttgaagtt
    17101 cgttcatgac caattctccg attaattctg tagaaacttc attttgaaat gtattgtaca
    17161 attgtttttc taccttgtcc gctatttgtt caatttgttc aagacttaca gatcgtttgt
    17221 cgcaggcacg gataagaccg tttattattt tatctctgtt atagccctgt cttgtgccat
    17281 cccgtttaat tacaacaatt tgagtttctt ctattttttc gtatgttgta aaacgatgtc
    17341 cgcattctat gcattccctt cgacgtctga ttttgaatcc gtcatcggta ggccttgtat
    17401 caataacctt gctttccgta taattacact ttggacatct catattttcc tccctcctgg
    17461 gtgacattcc ctgatagtat gactaactat ggttgaccgt caatcaagtg tgatcccata
    17521 ctacagcttg ctcatgaatc tttcgtcatt aataacaaat ctttcgtggg taccttcacc
    17581 tatcattttg tcttcatcat atgctgttac tttaaaaacc agcctctttc tgtctatatc
    17641 aatgagttcc gcctcagccc acactttttt gcctattctc gttgcactca tatgttttat
    17701 attaaccatg gttccaacgg ttgagtatcc ttcctgcaga taatccttca ctgcattcat
    17761 ggaagctgct tccataagcc caatcatcat aggggttgca aacacttcag cagcgccgct
    17821 gccgaaaact ctcgccgtat cttccatttt aacaattttc tcttccttgt attttaaacc
    17881 cttggataag tttaattcca ttacatcctc ccaacatcca tttttcttat tatacaataa
    17941 aattatcaaa aataaaagag gttttgaaat caaaacctca aacacttata aatcgattct
    18001 actgtttatg ctaaccagta aagaattaca aaagtaataa gcgaacattt gcaggcacga
    18061 ttataatact ttgcgaatga aggttaaaaa ctcgttaagc agcttgcatt gtttgagcgg
    18121 agcgagtttg catgctttag gttttcagcc tgagctttag aatttattcg tgtccaaaac
    18181 cgtcagcggt tatttttgtt atttttattg tcttttcaaa gcttcaaccg gaggaagatt
    18241 cgatgcgttt attgccggat aaacaccaaa cagcaatcct gtaaccaaag caacaaatac
    18301 agctatctgt accgactcaa agcttactac tgcagaaagc ccgtatcttg ccacaatact
    18361 taccgcccag gcaccaagta atactcctgc aaaacaaccg attatgctta catagactgc
    18421 ttccagcaaa aactgtatca ataaatcttc cttctttgct cccagtgctc ttcttactcc
    18481 tatttcgctg gttctttcag taacagcaac gagcattata ttcataattc ctataccgcc
    18541 taccaggagc gataccgccg caatgccccc aaggagtaca gtcattattc tgtttgcctg
    18601 gtctgcttcc ttaactaaat tatttaaact tgtaattgta agaggctctt cacctttttc
    18661 aaatacattt tcgtcctggc cgggtatacc gggttcttca ggcatgggtt tttcaggcat
    18721 gggctccgta ggcatcggtt cttctattgg catatctgcc ggcatgtctc cgggcatatc
    18781 tcctccatca cctggcggct gctctccttg tccgttatcc ggtttaatgt tgcttcctaa
    18841 acccaccttc cttttatata ttctccccag ctgaaccatt gccaattcgg catcttctgc
    18901 gctgttggat tttgcccata tttcaagtat atctctttca tttgtaattt tcattgcaga
    18961 agtgtagggt attacaattt tgtcatcaat accatcaccc ttgccttctc ccttttctcc
    19021 caaaacacct acaattctgt agtttatgcc gttaattttc atggtttggc ctacagggct
    19081 tcttccgtta agaagtgaat tggcaacatt gtatcctaat accacaaccg gggatctttg
    19141 ttttacatgc aatgatgtaa aaaagtttcc gcttataaca ggatggtcct ttatctgagg
    19201 atagttttca ttcactccca caatattcat ttcactttca tctctacgcc atcttatggg
    19261 tgtgtcttct gtatataaga ccggagtagc atattcaatg ccggataccc tgtcaggaag
    19321 tgtttctgct tcttctattt caaattttac attttccgca tttgatttta taacaataac
    19381 attttcgcct aagctttcaa attgtgctac tatagatctt cgtgccccct cgcctatgcc
    19441 cgtcaggctg attacagcag ccactcctat ggatatgccc agtactgtca aaaacgtcct
    19501 gagcacattt gatgttatgc cgtctaatgc catcattgca gaaaatttca accttaccaa
    19561 aaggtttgaa aaattctctc tgtcttgcat tatcatcgct tcaccactcc ttaaattgtg
    19621 cccggcagga ttgaatcatt cccgggtata gactgactgg gcatcaaatc tttagtggag
    19681 cctgtaacaa caagctgccc ttcttccagg ccgcttaaaa cttccacata catatcattc
    19741 attaagcctg tttcaatttc aactatttct acctggtttt ctcccgtaag gatttcaacc
    19801 ttttgttttc cgtcttcttc aaatacagct tcaatgggaa ctagcagcgt atccaaggat
    19861 tctcccgagt caacgaaaca attagtgttc atgccgggcc tcaatccttc cctgccttcc
    19921 acttgtatct gcacattgta tatggtcttt ccggtgccgt aatctgtata tgaatccagt
    19981 ctcatgacct tgccctggaa tgtttctccc gggagagcat caacagttac cgtaacaggt
    20041 gcgtcctggg ctacatacat cacatccaaa tctgaaactg tagtgtaaat aaacatttca
    20101 tccgtattgt aaacatccat cagcatcaac gcccattggt cactgttctc gttggccatt
    20161 atttgatctc cctgcctgta attaacataa gcaaccattc cgtctgtagg tgccgtaacc
    20221 atcatcttgc cggtaagctc ggagactttg tcgatttttt tataaatact gttaatttca
    20281 tcgtaagcat ttgatatagc atcaacttcc tgctggatta tttgagttac atcacttcct
    20341 gctattctta caagaaggtc tccttcttca acaaattcat tggtgcttac aaaaacttca
    20401 gatgcaatga cgttagagct gtcgccgccg ggaatataag aatatgttat cttcttttca
    20461 tttacaaaag attctacttc tcctggataa gaaaaggcgg ttacaggatt tccgttattc
    20521 tcggtataga tagtaacagt cattcccggc tgcacaagac ccggattgtc cgcttctata
    20581 gttccattgt ggacatatgt cattccatta ggcactttgt ttttgtttaa tttagttata
    20641 gttcccggat agaagccctc aaaggctaat tgcgacccat gctctgttgt ccttcgatac
    20701 tgcagcaata ccttctgtcc ttccttcaag ttcccatatt catatgtatt taccttgaaa
    20761 ggaattttta ccatgctgtc gtcgacaata gtagcaatat tgaagttatc aaccttttcg
    20821 ccttcttcca catttagctc tgtaatcctg ccccggattg gtgccctgaa aactattccg
    20881 tcataagcat taacctgatt tatatttgaa atatcctgat ttaatatttt ttccaaattg
    20941 gacattttcc cctggatatc ttcctgctta gcttgaatct ggtcggtaaa tccagtcaaa
    21001 atatcaccta gattggatgc agaaaggcgg attaaattat ctcccttttt aatcatttga
    21061 ttcggctcaa caaaaacctc ttcaacagta tatgtaacct cgccaattcc ttcgggctta
    21121 ggcgcagtta tggaccctcc ccagttgccg cttagctgtc cggaaatgct tacaccttgt
    21181 tttatatctc ctctttccac agcctttgta gaatacctgg ggcctgcaga agcttctgag
    21241 tcatcaggaa tcatccactt ggccgttaaa tatccgccta aaaatactaa tgctattact
    21301 attcctatag atataaattt tttatacatt tttaccctcc actacttcat cagatactat
    21361 ttgaccatcc acaagtctta tgattcttga accgtattcg gcaataaatt cttcatgggt
    21421 tacctgaaca acggtcattg agcttttttc gtttaattct atgaataatt ccattaccat
    21481 ttcaccagat tttgaatcta atgctcctgt aggctcgtca gccagtataa tagacggatt
    21541 tcccgcgata gccctggcta tagccaccct ttgctgctgg cctccggaca attgtccggg
    21601 cagatgtgtc atcctgtcct tcaagcctac ctgctccaat gcattttcag caagctcttt
    21661 cctctttttg tggttcattc ccctgtatat taaagggatt tcaacatttc tcaacgcatt
    21721 catcctgggc agcaaatgaa agctttgaaa tacaaatcct atgaatagat ttcttgtttc
    21781 agacatctta gaatcgctta atttattaac ctctgtcccc gctaattcat aggttcctcc
    21841 agtaggatgg tccagacatc ctatcaggtt taaaagtgtt gatttccccg aaccggaggg
    21901 gcccataatg gaaacaaatt ctttttcctt tatattgaag gaaatatttt ttaaagcttc
    21961 aacttgtatt tggcctgatt tgtacagctt ggagatattc tccattttga taatatccat
    22021 cttccacctc tctttgcaca taagtccata tattagacgt attgatttaa gaaaatgttc
    22081 aataaaactt aatatttttg taaatgattc aggggacatt tctcatgagc caaatttggg
    22141 gacattcctt cgaccagcaa agaatatctg cttggagaag gtgtgtcccc atacctctta
    22201 taaaaaggaa cgaccgggtc gttccatata tcgttttatt aattatttaa taacatnnnn
    22261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa aggaacgacc gggtcgttcc
    22381 atatatcgtt ttattaatta tttaataaca tcaattaata aggaatcaat ccacacttta
    22441 ttttcacctt cgtattccaa ataaacacat aaaatgtcgg catcagttac atttataggc
    22501 actgtaaact gtgaccagat atcatcatat gaattaacca gtgaatgaaa gtttgacttc
    22561 atgaccccgt ctttatatgc ctcgaaaaca acttgaacct gagtatcata ttgtgatttg
    22621 atttttcccg aaacaccaat ttgtttcatc agcatatagc ttacatctgc atctgtaaaa
    22681 tcaacagctg caaacagtgc cttgactttg tcctgtccgt cattgacaac taaaagggat
    22741 gagttgcctt caacatatat ttcggtatct ctttgggctg cagcaccttc atatcctata
    22801 ctatccagca gttcttcgct gaaatgaata tctgtggatt ccaatggagg tttttcctcg
    22861 ccgggctgcg aaggtgtttc ctgtgccggc ggttttccgg gatttattgt ttcagttgag
    22921 ccatccttat ttttgtttaa tccgttgata attgcatttc cggaaagcag gaccataact
    22981 actataaaca atgaaagggc tgctcttttt atttctttgc ttaaagatat tttcttttta
    23041 ggaggttttt catcacccaa gtaaaggttc agaatatcct cggagctttc atcatgtata
    23101 ataatggatg gattctcggg tttttcattt tctttttcta tcccaatctc ttctccgaaa
    23161 atcattataa aaataggcga tcttcttaat tcaatcaatg catcgtttgg aaaaacatat
    23221 tccctggtaa ggcatctcac tattattttt aatatgtcag gaggtatgga gtctgatata
    23281 ttatagtcaa caatttctgt gccggaaaaa atcatgtgta taatactgcc taatctttta
    23341 aaggtttcat tgtcggctat atcgtattcc tgttcaaata taaggttgca gtcaacaatc
    23401 agctcgttat tgtcatcaat gtacagccgt tttaaatcca aaattttctg ctgcactaca
    23461 tcagtcatat tgtaaatact agaggccagg tttattgact tttctgccag gttgaactgc
    23521 tgtttcatgg aaaggtcagt gcttgccagg tctgttagtt ttatgcagtc tgcttctaag
    23581 gatttagtga atatatatat tcgatcctcc gtattatagc attctataat attggagttt
    23641 aattttttca aatcctggag atttattaga ttaattacct ttttgcttgt tattatattg
    23701 gagtaaaaaa cttcacctgt atcattgtct ttgcattcca aaattctttg gaatacattt
    23761 tcataaatag tacgaataat cgtgtaattg ttattcataa cagacctcca aatattatta
    23821 actatatatt aacaataatt tcgacaaaaa taaagacatt tttgcaatat ttcacaaata
    23881 aatgaggatg agttattttt cctcatcctc gtgtatttta tattcaacat cttcaataat
    23941 atctttacct tcattcttat tgtcgtcata atatttttta gttttctcat atattattgt
    24001 caataaaaag cctagcatcg tcaagtcatc cactatacca aggcctagta cgggggcagg
    24061 tattaaatcc acaggactca gaatataaat aacaggtata atcacccacc acttttccct
    24121 caggggaaca tttttatcaa ataaaaactt taatataaat aaaactctgc ccataatcgt
    24181 atcctaaatt atgaaatcaa cattgggaag ttttttttgc agcttggaaa taaaactgtt
    24241 tgaagaattg tttgagtgac ctactacaat atgggtacag tttatttctt ctgcgtagct
    24301 gacaattgtt tttataacat cctttgatct tttaacgact aagttggcag cccccacatc
    24361 tttagttatt tcaaacaggt attccaaggc atcaccatcg cttaaaccat ataaaagctt
    24421 gtcgttttca tttactacat gtataacgtt taagcagctg tctgcttctc cttttgtcaa
    24481 ttccacccct ttttttatta atttctcgct tgttttttgt ctagttacac ataccaatac
    24541 gcaattaggc ataatatttc tccttaaaat ggcgggaata tgtgggaatt gaacccaccc
    24601 aggacgactc cgccccgcat ctgattttga agaccaggag gcacaccagc acctatctat
    24661 ccccactaat catactatca taataattta attaattcaa gaactaaaga taattggatt
    24721 aaaaaaaaca gctgcaaaat ttcgcagctt tatatacgta tcaatcttat atatgaagat
    24781 aggattccca gtcttaaaaa tacagggttg gcaggaaaat ttactgataa ttgtgccagg
    24841 tcttaaaggt catcagtttt tagttctgaa ataatatcct acaccccatt ttgtgagtat
    24901 atactcataa gctttgtttt tattttctat tttttctcta agtcttctaa catgaacatc
    24961 aacagttctt aaatcgccaa agtattcata gccccatatt atttccaaaa gctcttccct
    25021 ggaaaatacc tttcccggat ttgttattaa taaaagcagc aaatcaaact cttttgcagt
    25081 gagatttatt tcctgattat gaagacttac tttccttcct aaagcattaa tggtaaagtc
    25141 atccaccact aatgtctgtt catttgcttt ttggcttgaa ttccttgtcc ttcttaaaat
    25201 tgcttttatc cgtgctttta actctaaaat attaaagggt ttggaaatgt aatcatcggc
    25261 accatattcc agtgctaata tttttgtcat gtcttcattg tttgctgaca aaattaaaat
    25321 cggaacattt gaaatttctc taattctttg acaaacttca aggccgtctg tttcaggaag
    25381 tataagatct agtattattg catcatactc attggtaatg atccttttta acgcgtcatt
    25441 gctgtcatct gttgattcaa cctcgaaatt atcctgttca aaactgtact taagattttt
    25501 aatggttaaa agttcatcat caacaatcaa gactctcatt tgttacactc ctcaaaataa
    25561 atgttacaaa aactcaaatt gtttattaat tttacataat aaaacaactt ttgtcaagaa
    25621 atttattatt ctattgtaaa attcaataga attctatcat tttctactct ttttgtaagc
    25681 tttaccatgt cgaaatgctc cagaagcgtt attgccgtct tcctgttggt attaagcaaa
    25741 tctctatatt gagcagcact tatttttttg ttctttttta aaaattcaac cagtatgttt
    25801 tttgcttctt catataagat tttgcttgta accaactcgt ttattttcac caggtctttt
    25861 tctgccatca ttaaagataa tacatcattt gtcataggaa gacctatctt ttcatccaac
    25921 tcttcaaatt ttaaaaattc aaatttaacg tcatttagaa tattcaatgc tttttccttg
    25981 attcttttgt aatcctcatc atattcaacg gtgaaataag gaagcgaaat aaactcatcg
    26041 gtctgtttta tca
//
LOCUS       DAOPUT010000015        21737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_2405_length_21737_cov_21.559589, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000015 DAOPUT010000000
VERSION     DAOPUT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 21737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21737
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_21737_cov_21.559589"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1037
                     /locus_tag="PKK75_02875"
     CDS             75..1037
                     /locus_tag="PKK75_02875"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HNZ82568.1"
                     /translation="MNRNLKTSNITESAMITGILVIIAYLSSFITLLMFFYPAPAIIL
                     GRRKGFKYSILALIASDLIISMLLGIQTGLAFLLLYTPLAIALTYGVCRDEDANKTIL
                     YGSAAYMISFVIYILLLDLIMGINFIERVAEMYDQSFEMSRGLFDNIPDQYRTGQIDR
                     YIKDIEEMAPMMNYIITNIFPAVLIAASVITSYINYVVASKFALRFSINVKQHEGIGY
                     FTFPRNFMIAMAGLLLLSFLLNLLNINVGIIQLNLFIIIFMAMLLQGFAVMKFFIDRS
                     SLGRFARNLIMVVIVFMATSFSVVYSILGLIDLTVNLRKINRAV"
     gene            1052..3055
                     /locus_tag="PKK75_02880"
     CDS             1052..3055
                     /locus_tag="PKK75_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNZ82569.1"
                     /translation="MDKKTPVFLKDDSFIYFIVIVLLIIIFLLEGMFIYAASAGLVLI
                     LITAYTLRKQILRKKELKNYIINYTKNLENLSVNSFYYSPLPICIIGLNGKMFWFNNK
                     FKELLKDSSDAIENIEEFIGGFPLNSMDGNKEGSINSIEVPDTGRSFTVLYYELEEGR
                     FGEGKSYVCYWNENTAFVTLRNKYNDERPITMFIQIDNYDEISEKLELGEKSSMTAQI
                     EKILNKYASEMNGFVLKYDNSKFFMMIENKYLETLEAKKFSMLDDVKSIKGTNDFNFT
                     LSIGTGAMAKNLTQLLDYSKGALDVALGRGGDQAVVKRINSVKFYGGKSKAVEKRTKV
                     RARIISYALRQIIDQSSNVIIMGHRVGDMDSLGASIGVCSIARSRGKKSYIVLNNVNH
                     ALKNMHERLKKEEGHYIESLVTTEKALEVADQNTLIVVLDTHRKSYTEAPELLDQIDK
                     VVLIDHHRRSEEYIENTLLDYIEPYASSTCELVSELIQYMEEHIKLTKFEADALMAGI
                     VVDTNSFTFNTGIRTFEAAAFLKRRGADNVEVKELFNESIEFMKKKTEIIERSEIVFD
                     DVAVSYIDEYTDDSNVAAAQSADELLTIKDVKMSFVLVRNKEYINISGRSVGDENVQV
                     IMEYLGGGGHLNMAGAQIDTGDMNEAKEKLYEAIARYKKESSK"
     gene            3052..3498
                     /gene="rplI"
                     /locus_tag="PKK75_02885"
     CDS             3052..3498
                     /gene="rplI"
                     /locus_tag="PKK75_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNZ82570.1"
                     /translation="MKVILLEDVKNVGKKGQIINAKDGYARNFLFPKNIAIEATEVNL
                     KNLENAKKKKEEKEREAFEEARVLEEELMQKVIVIKTKAGENGKLFGSITTKEISELL
                     EKEHKIIIDKKKFDLDEPIKTLGEYNVKIKLHPMVNAKLKVIVTEK"
     gene            3520..4842
                     /gene="dnaB"
                     /locus_tag="PKK75_02890"
     CDS             3520..4842
                     /gene="dnaB"
                     /locus_tag="PKK75_02890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNZ82571.1"
                     /translation="MNIGKIPPHNAEAEQTVLASCMIDHIAVEKVVNLLGYDDFYYEA
                     NKEIYDSIKQIYMQNIPVDAVTVFEELKKRGKVDYVGGFEYLATLTENIITSKNVEAY
                     CNIIREKSTLRKLINASQEIIEKGYKEDDDAQKIIELAESRIFSISQNRSINTFSDIK
                     EVLMTVFNQLEERAKSGGGITGLATGYTDLDRMTSGLQKSDLILLASRPSMGKTALAL
                     NITMNVVKSGASVALFSLEMSEEQYVQRIISQESMVDSTKLRTGNLDDDDWTRLISTM
                     SLISSYKVYIDDTPSVTLFELMSKCRRLKIEKGLDLIVVDYLQLMSDGTGRTDNRQQE
                     ISNISRGLKALARELDCPVMALSQLSRAPELRGDHRPVMSDLRESGAIEQDADVVMML
                     YRDEYYNKEESEKKGITDVIITKQRNGPVGTVELAWIGQYTKYGNIQK"
     gene            complement(4933..6480)
                     /locus_tag="PKK75_02895"
     CDS             complement(4933..6480)
                     /locus_tag="PKK75_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HNZ82572.1"
                     /translation="MVKLFNDTFNINEVVDSFSTEVLLENEITIADDMPDMEKIVNAE
                     GKAKLDNVRATTDSIIVDGRLKYNIIYRSNNENYTIHSMTGEIPFREEISAEGVTEDM
                     EAFTNVFIDYIDADQLTERSFLVKAVLILDTDAYMKRPVAYVSNLESDGTIQAKSRNI
                     AYTDIAAEISEEININDAVELSKGSGEIAQVIRSEADVYITNIDELNDKMLIEGICKV
                     GFMFIEDDEMHTIGYVSEEFPFTHYLESEFSNENMLKDIGVNVNDMTYSITENFDDEK
                     KLIEFALPFTVNASFYKTMEKNIIMDCYSTKYGLDLQSETVKLSSLKNLTNEVVKYEN
                     NFDLVSGSIKDIYSVDASPKVSEKRIADNKYIIDGFLDVNLLYLNGDINKIDKAFASL
                     PFTAAFPVDEDEALSNIHSKVSVHKCSAYRKGNSSVNFNCDINVGMKFKNDDEVTVIK
                     DIAENNPIDRSKMPSLIFRVVQSEETLWDIAKNYNLSISYLKELNDFPEGSVLEPGSK
                     IIIARSV"
     gene            6870..8471
                     /locus_tag="PKK75_02900"
     CDS             6870..8471
                     /locus_tag="PKK75_02900"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNZ82573.1"
                     /translation="MPKYIFVTGGVVSSLGKGITAASLGRLLKSRGLKVTLQKFDPYL
                     NVDPGMMSPYQHGEVYVTDDGAETDLDLGHYERIVDIDLTQYSSITSGKIYWSVLNKE
                     RRGDYHGGTVQVIPHVTNEIKRILKRIATVEDVDIVITEIGGTVGDIESQPFLEAIRQ
                     IRYEVGRDNVLYIHVTLLPYLSMAGELKTKPTQHSVKELLSIGIQPDILVCRSEKEIS
                     AELKTKIALFCNVEKQNVIQNVDADSIYDIPLMLEKEGLAKLVCDHFNLNCAEPDLAE
                     WTQMVDRSKSPKHSVKIGLVGKYVELKDAYLSVAEALYHAGIDNSCDVHISWIHSEQV
                     NENNYKQILEDCDGILVPGGFGDRGIEGKILAAKYARENKKPYLGIGLGMQMALVEFA
                     RSVLGYSDADSFEFNPESKYLIINKADGHNDIESNGGYMRLGSYPCRLIDGTKVKGAY
                     GGADMINERHRHRYEFNTEFRDVCEKNGLIISGVSPDNKLVEIIELKNHPWFVGAQFH
                     PEFKSRPTRSHPLFKYLIKASIECK"
     gene            complement(8568..9455)
                     /locus_tag="PKK75_02905"
     CDS             complement(8568..9455)
                     /locus_tag="PKK75_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ82574.1"
                     /translation="MKKPSKYWILAGTFFTSVSSIIIRFSESPSLVIAAYRMLFTSIM
                     LFFPVYIKYGSEFKSLNKNDCLLCLISGIFLALHFASWIQSIQMTTIANSTILVSCSP
                     IFVAAINYFLLKERITGKMIVGIGMSLIGTVIIGIGSSQGNGTAMILGNVLAFMGAVF
                     VAGYLVIGGIVRKTVSAGVYVLIVYSVSAIALFFMCFASGTPVYPYPLKEFALFFLLA
                     FFSSILGHTVYNYLMKYYSSTLISITTLMEPVFASLMAMIIFKEIPSAYTVLGGIIII
                     GGIYLGTYFSQEKPAGDGS"
     gene            9598..11040
                     /gene="cls"
                     /locus_tag="PKK75_02910"
     CDS             9598..11040
                     /gene="cls"
                     /locus_tag="PKK75_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNZ82575.1"
                     /translation="MELFNSITLIYAVNICMAIIVIFLERKDPTATLAWVLVLLLFPG
                     FGFLLYLLLAQNFSRKQLFIMKIYAKKSFGDYIRVQRELFATGGLVFNDKNVESYKDL
                     IKMNLFYHGFSYTQNNEVTIYTDGEKKFDELFRLIKEAKSHIHMEYYIMRNDTLGNNL
                     LNLLSKKAQEGVEVRLLYDSVGGRQITGEQIENLRRSGVKVAVFFASTIPFFNFKINY
                     RNHRKIVVIDGITGFVGGFNVGDEYVGLNNKIGYWRDTHLKIRGDAVIDLQTRFFLDW
                     GHASKEEVLFLPKYFPDNRIQGRVGIQIISSGPDSLDEAIKSNYVKMINSAKKSILIQ
                     TPYFVPDASVFEAIKIAAASGVDVKIMIPCKPDHPFVYWATYWYCGGLLKYGVKVYTY
                     EYGFLHSKTLVVDGTVASVGTANFDVRSFKLNFECNGIIYDRDTSQMLYDIFMEDLNY
                     SMELTRELYLERGILIRFKESVSRLLAPLL"
     gene            11124..12014
                     /locus_tag="PKK75_02915"
     CDS             11124..12014
                     /locus_tag="PKK75_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNZ82576.1"
                     /translation="MKRYFVIYNPSSGKELAAYKVFGAAETLMSVEDAEFSFYSTKKK
                     GDGEEAAIRACRLDYEMIITCGGDGTVNEVVNGMMKCSNRPKLAIMPAGTVNDFAEQL
                     NLPKAQENFASLIMQNKYSTIDVGKINEKYFINVVGGGAFTTIPHRVTIDAKTILGKY
                     AYYYQAAVKVPDELEICEEIEYIIDGKKLGYNTLLFLVLNSAGAGGFKYLCPKAKLDD
                     GLLDIVIFEKTNYAEMFQIFTKVFNGHHITHPKVHYFQAENIKIIPEKELLIDADGDP
                     AGYAPAEVSSVHNAIDILVP"
     gene            complement(12032..12721)
                     /locus_tag="PKK75_02920"
     CDS             complement(12032..12721)
                     /locus_tag="PKK75_02920"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNZ82577.1"
                     /translation="MIGIIGAMGIEVEDLINSMDLIKKETISGIDFFEGKLQNKNAVL
                     AICGIGKVHAAVCTQTMILKYAPDAIINIGVAGCLSPELDFADIVISDSVVQHDFDVT
                     PFGVPVGLISGIDLIKIPCSDRLVKKLVKAAEILEDTNIAVGTVASGDQFVGSKEKKD
                     YIVNNFDALCTEMEGAAIGHVCYMNNVDFCIVRAMSDKADSTANMDFDIFVKNAIDKT
                     IKLINRFLKVY"
     assembly_gap    12783..12882
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13113..13349
                     /locus_tag="PKK75_02925"
     CDS             13113..13349
                     /locus_tag="PKK75_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNZ82578.1"
                     /translation="MHYIYILECRDKTLYTGYTNDLDKRIKTHNEKKGAKYTRGRTPV
                     VLKYYEEFDNKIDAMKRESQIKKLRRVKKLMLFK"
     gene            13421..14245
                     /locus_tag="PKK75_02930"
     CDS             13421..14245
                     /locus_tag="PKK75_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ82579.1"
                     /translation="MESNLIVDSCTDFNEETLDIPRVPFRIIIDDEEIVDEDTDIIEL
                     IRKMKSSRNQIKTACAPPEDFINKMKVQANNFIVTISSQLSGSYNSAMVAMETIKEKF
                     PETFVHVFDSKSATAGETLIALKVKQLIEENLNPHEIVEQVEKYIPNMRTLFILDSLD
                     NLAKNGRISNFKALLANIMNIVPIMGEDGEGRIILKEKVRGKKKAFSRLVEILDEYNI
                     DYQNTVLGITHVNCLEKAENLRDAIKAKYNFKDIKIFNPAGLSTVYAYDGGIVIAF"
     gene            14315..15172
                     /locus_tag="PKK75_02935"
     CDS             14315..15172
                     /locus_tag="PKK75_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ82580.1"
                     /translation="MKYKEIISEIKSVRLYKKDQVDTKLFDELKQYYEQGKRLVDDIE
                     MEVLRKNRDEVFEQLKDSAGYNNRMIEAPHYLIVLSEEKDHYIENAGYRVQDIMLKAW
                     SLGLASCWITFEDGEAIKKKLGINTDKKLAALIALGYEDPQNKVLYEKGKYNTSSNVK
                     VIEDNTSERLGIRDLVFTKEWGQKADPDELVNYGLLEGFFYGILAPSYKNRQPWRFIV
                     DNGTVVLALKKDIYVTEYEEKIDTAVIMLYFDAIIESTLHDISWKFGKPEKDYKVPDD
                     YKIAAYCII"
     gene            15255..16163
                     /locus_tag="PKK75_02940"
     CDS             15255..16163
                     /locus_tag="PKK75_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNZ82581.1"
                     /translation="MKIIIHKKYLDLVSEKFPDIEFYTEIDDCPEAEALIGEPMDFVP
                     EKLDKLPGLRWIQSFRAGYDTLDLDYMMKRNITFCNGKDIYSIPIAEDVVCKILMHNT
                     NAMAYLKSQKDHKWDLSQRRTEFQGKTIGIIGTGSIATEIAKRLQGFGVRIIGYKRSP
                     VSSMAYFNEILSGKKGLEYLMSESDYIIVTVDLNKETYHMINKDTLKYMKESAAIINI
                     ARGSIINQKDLTQALRNKSISYAGLDVFEVEPLPEDDELWGLENVYITPHASGIVREN
                     KKRLGDLVVSNIQRFIDGEKLAYAVK"
     gene            16222..18195
                     /locus_tag="PKK75_02945"
     CDS             16222..18195
                     /locus_tag="PKK75_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ82582.1"
                     /translation="MNTNKNILKILFFPLLILYYETILRIFLFDAVFNMGYIYMCLFS
                     FPLGLFFYLLSKAFNEKTNKILFYVIVIFLTLYYCVQIIYYRIFNTFTSWYSILAGTG
                     KVLWFKDIIISVLLDNIFELIIVFLPLVLLLIFHRNLQFNKIIKRYAVNIALSALVMQ
                     TAIVIAVLSSDTGVLSPSYLYSETFLVVESVDKFGLLTTGRLDIQNILKSLVFAIEIK
                     PPEIIDEPSAEPQDEDIPKPPQEDPSAGIAEYNTMDIPFDDLINTEKNQAIRNMHEYF
                     SSVQPTKKNEYTGRYEGKNLILITAEGFSPYAVRKDITPTLYKMQEEGFKFKNFYTPT
                     WGVSTSDGEYVACTGLLPKEGIWSFYKSSANFMPLAMGNQLKKEGYETKAYHNHYFDY
                     YYRNMSHPNLGYIYKGLGNGLDITETWPESDLEMIELTVPEFIENEPFHTYYMTVSGH
                     LQYTFTGNYIAAKNRSLVEALPFSNNVKAYLACNIELDKAMERLIQELEKKGIAEDTL
                     IAISPDHYPYGLTLAEINELADSEIKGDYDLEKGVFLLWSKGMEPVEIDKPCSSLDII
                     PTLSNLMGLDYDSRLLMGRDILSDSEALVMFSDRSFMTDKVFYNARTNEVKALTGEDI
                     EDSYIKERADTVLSKFKYSALILDSDYYRRILD"
     gene            complement(18307..19167)
                     /locus_tag="PKK75_02950"
     CDS             complement(18307..19167)
                     /locus_tag="PKK75_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82583.1"
                     /translation="MKLMKECTIYIDVPEKYLKGAGILNSQFIPSYIYQIKIILKNTA
                     DIEKLCSFSAALANGIRYLDNLNHAGPDTEILDNVSIVEPCKELNNDNVIIFGNNFTL
                     SPNSTNTIVFDTALCDKYTAESIENSGDKIPHRSKISFYANLINGGITDSYSAFSTAM
                     DFDMSVECNDERLKPGDITKYYVNLSTGQYDLARKVYLRSILDGALEYIGDSCNLEPK
                     NVYEFNGKTIIKWDVGSLLPSEIKRIGYKVKVKKDAEGKLTNKLNSNCINNSAYTQCP
                     VSLKHVLDVD"
     gene            19311..19535
                     /locus_tag="PKK75_02955"
     CDS             19311..19535
                     /locus_tag="PKK75_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNZ82584.1"
                     /translation="MKNIKVYSSSTCTNCTAAKEYIKEKGYKYEEKNVSTDMEARKEL
                     IDMGYMGVPVIMIDDEVVVGFNKGKLDEIL"
     gene            complement(19612..19794)
                     /locus_tag="PKK75_02960"
     CDS             complement(19612..19794)
                     /locus_tag="PKK75_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNZ82585.1"
                     /translation="MITKDMTIREVIMAKHEAAEILMAFGMGCVGCPSAQMETLEEAA
                     AVHGMNLNNLLAALNK"
     gene            20116..21006
                     /locus_tag="PKK75_02965"
     CDS             20116..21006
                     /locus_tag="PKK75_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82586.1"
                     /translation="MQKISDMKKCIQKKSKLNMLLKQTEQDIIKEKLLINKLSAELEK
                     ESQDILNLKSNNIISLFQTVLDTKEDKYNKEKRDLLKARLKYDQCKNNVNYLVEETKK
                     IVDYIAELNGCDAEYEELINKKLETINMADDETGLELKRLIKRKENINANMVEIYEAI
                     CYGEKALKAIELAIKELEDAEDLENIDERGNIDNARYHAEQTQRMLSMFKKELSDITM
                     FTGTEIAAGTFENFSDCFFDSLIFDWAVQSGICKSLDTVKNTKNQLDKAISKLYEEKV
                     TEEFMAKQIEEQINQIIDKA"
     gene            21078..21545
                     /locus_tag="PKK75_02970"
     CDS             21078..21545
                     /locus_tag="PKK75_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNZ82587.1"
                     /translation="MSVEKVRDYLKQFNKGNDILEMDESTATVELAALALDTEEARIA
                     KSLSFYDGDGAMVIVTAGDAKIDNRKFKDYFGFKARMLAPEDTEKFTGHAIGGVCPFA
                     LPKNVKVYLDESMKRFETMFPACGTGNSAIELTLEELEQTSQSNTWIDVCKNW"
BASE COUNT         7362 a         3619 c         4354 g         6302 t
ORIGIN      
        1 gccaccttga aataagcttt tgactttaag ctgtttatgg ggtaaaatat tgtaattaga
       61 tatgagaggt aatgatgaac agaaacttaa aaacatcaaa cattacagaa tcggcaatga
      121 taaccggcat attggttata attgcttatt tgtcatcttt tattacttta ttaatgtttt
      181 tttatcctgc tcctgcaatt attctgggca ggcggaaggg ttttaaatat tcaattttag
      241 ctttaattgc ttctgatttg ataatttcca tgcttttggg aatacagacg ggacttgcat
      301 ttttgctttt atatacacct ttggcaattg ctcttactta tggagtatgc agggacgaag
      361 atgcaaacaa aacaattttg tacgggtcgg ctgcttacat gatatcattt gttatctaca
      421 tattactttt agatttaata atgggcataa acttcatcga aagagttgca gagatgtatg
      481 accaaagctt tgaaatgtcc agaggtttat tcgacaatat tcctgaccaa tacagaaccg
      541 gccagataga ccggtatata aaggatattg aagaaatggc acctatgatg aattatatta
      601 taactaatat atttcctgca gtcttgatag ctgcatcggt aatcacatca tatattaatt
      661 atgtggttgc cagcaaattt gcactgaggt tttcaataaa tgttaaacag cacgagggta
      721 tcggttattt tacctttcca agaaatttta tgattgcaat ggctggcttg cttcttttat
      781 catttttgtt gaatttgttg aatattaatg ttggtattat tcagctgaat ctatttatta
      841 taatatttat ggcaatgctt ttacagggct ttgcagtcat gaaatttttt attgacagaa
      901 gcagccttgg caggtttgca agaaacctga ttatggttgt tattgtgttt atggccacga
      961 gcttcagcgt tgtatattca attttaggat tgattgattt aaccgtaaat ttaagaaaaa
     1021 ttaatagagc cgtataaatg aaggaggtat tatggataag aaaactccgg tgttcttaaa
     1081 agatgacagt tttatttact ttatagtcat agtcttatta attattatct ttcttttaga
     1141 aggtatgttt atttatgctg cttctgcagg tctggtgtta attttaataa cggcatacac
     1201 attaagaaag caaattttgc ggaaaaagga actaaaaaac tatattataa attatacaaa
     1261 aaacctggaa aacctttcgg taaattcttt ttattactcg cccctgccca tatgcataat
     1321 cggtctcaac gggaaaatgt tctggtttaa caataagttt aaagaactgc tcaaggattc
     1381 ttcagatgca attgaaaata tcgaagaatt cataggagga tttcccttaa actcaatgga
     1441 cggaaacaag gaaggctcaa taaacagcat agaagtaccc gatacgggca gaagcttcac
     1501 tgtattgtac tatgaacttg aagaaggacg attcggcgaa ggaaaatcct atgtgtgcta
     1561 ttggaatgaa aatactgcat ttgtaacctt aaggaataaa tacaatgatg aacggcctat
     1621 tacaatgttt attcagatag ataactatga cgaaatatct gaaaaactgg aacttggcga
     1681 aaaatcatcc atgaccgccc agatagaaaa aattcttaac aaatatgcta gtgaaatgaa
     1741 tggttttgta cttaaatatg ataattccaa attttttatg atgattgaaa acaaatatct
     1801 tgaaacattg gaagcaaaga aattttccat gcttgatgat gttaaatcaa taaagggtac
     1861 aaatgatttc aattttacct tgagcatcgg tacaggtgca atggctaaga atcttaccca
     1921 gcttttggat tactccaagg gagccctgga cgtggcattg ggaagaggag gagaccaggc
     1981 agtagtcaaa aggattaatt ccgtaaaatt ttacggcggc aaaagcaagg cagttgagaa
     2041 aagaacaaag gtcagagcaa gaatcatatc atatgctctt cgtcagataa tcgaccaaag
     2101 ctcaaatgtg attattatgg gacacagagt aggggacatg gacagcctgg gtgcatctat
     2161 aggtgtatgc agcattgcaa gaagcagagg caagaaatca tacatagtct tgaataatgt
     2221 gaatcatgca ttgaaaaaca tgcatgaaag attgaagaaa gaagaaggcc actatatcga
     2281 atccctggtt acaacggaaa aggcattgga ggtagctgat caaaacaccc tcattgttgt
     2341 tttggataca cacaggaaaa gctatacgga agccccggaa cttcttgatc aaattgacaa
     2401 agtagtattg atagaccatc acaggagaag cgaagagtat attgaaaata ccctgctgga
     2461 ttatattgag ccctatgcat cgtcaacctg cgagcttgtt tcagagctga tacaatacat
     2521 ggaggaacat attaagctta caaagtttga ggcagatgct ctcatggcag gaatcgtggt
     2581 ggatacaaac tcatttacat ttaatacagg cataagaaca tttgaagcag cggctttttt
     2641 gaaaagaaga ggtgccgata atgtggaagt caaggaactg ttcaatgaaa gcattgaatt
     2701 tatgaagaag aaaactgaga ttatagaaag aagcgaaata gtttttgatg atgtggcagt
     2761 atcatatatt gatgaataca cggatgattc gaatgtagca gctgcccaga gtgcagatga
     2821 gcttttgaca atcaaggatg taaaaatgag ttttgtcctt gtaagaaaca aggagtacat
     2881 aaacataagc ggacggtctg taggggatga gaatgtgcag gttattatgg aatatctcgg
     2941 tgggggaggc catttgaata tggccggtgc tcaaattgat accggtgaca tgaatgaagc
     3001 aaaagagaaa ttatatgaag ctatagcaag gtataaaaag gagagttcaa aatgaaagta
     3061 atactgttgg aagatgttaa aaatgtgggg aaaaagggac aaataatcaa tgcaaaggac
     3121 ggctatgccc gcaatttcct ttttcctaaa aatattgcaa tagaagcaac agaagtcaac
     3181 ttaaaaaacc ttgaaaacgc caaaaagaag aaggaggaaa aagaaaggga agcttttgag
     3241 gaggccaggg ttcttgaaga agaactgatg cagaaagtta ttgttataaa aacaaaggct
     3301 ggcgaaaacg gaaagctttt cggctcaatt acaacaaaag aaatatcgga attattagag
     3361 aaagagcata aaattataat agataaaaag aaatttgact tggatgagcc tataaaaacc
     3421 ctgggcgagt acaatgtgaa aataaaactt caccccatgg tcaatgctaa actaaaagta
     3481 atagttacgg aaaaataaga ggtagaaatg tctgaattta tgaatattgg gaaaatccct
     3541 cctcataatg cagaagctga acaaactgtg ctggcttctt gtatgattga tcatattgct
     3601 gtcgaaaaag tagttaattt gttgggctat gacgattttt attatgaagc aaataaagaa
     3661 atatatgaca gcattaaaca aatctacatg cagaatatac ctgttgatgc agtcactgtt
     3721 ttcgaagagc ttaaaaaaag aggcaaggta gactatgtag gcggatttga atatctggcc
     3781 accctgactg aaaatataat aacttcaaaa aacgttgaag catactgcaa tattataagg
     3841 gaaaaatcca ccctgagaaa gctcataaac gcttctcagg aaatcatcga gaagggctac
     3901 aaggaagatg atgatgctca aaagataata gaattggctg aatccaggat tttttccatt
     3961 tcacaaaaca ggagcatcaa tactttttcc gatataaagg aagttcttat gactgtattt
     4021 aatcagcttg aagaaagagc caaaagcggc ggaggcataa ccggtcttgc cacgggctac
     4081 actgatttgg acaggatgac ttcgggactt cagaaatccg acttgatact tcttgcatca
     4141 aggccctcca tgggaaagac agcccttgcc ttgaatatta cgatgaatgt tgtaaagtcc
     4201 ggggcttctg ttgccctctt cagtcttgaa atgtcagagg aacagtatgt tcaaagaatt
     4261 atcagccagg aatccatggt tgacagtaca aaactcagga caggcaattt agacgacgat
     4321 gactggacaa ggctaatcag taccatgagt ttgatttcca gctataaggt atatatagat
     4381 gacactccat cggtgacctt gtttgaattg atgtctaaat gcagaaggct aaaaatcgaa
     4441 aaaggtctgg atttgattgt agtggattat ttgcagctta tgtccgacgg gacaggaagg
     4501 actgacaaca gacagcagga aatttccaat atatcaagag gattaaaggc tcttgccagg
     4561 gagcttgatt gtccggtaat ggccctgtct cagctttccc gtgcacctga actcagaggc
     4621 gaccacagac ctgtcatgtc agacttgagg gaatccggag ccattgagca ggatgctgat
     4681 gtggttatga tgctttacag ggatgagtat tacaacaaag aggaatcgga aaagaagggc
     4741 ataacggatg taatcattac aaaacaaaga aacggccccg taggaaccgt tgaactggca
     4801 tggataggcc agtatacaaa gtatggaaac atacagaaat aatgacggtt aaaattgaac
     4861 ggttaaaata aaaaaattca ggtcagacct gaatttttat tatactttta ttaatatgcc
     4921 gccaatatct atctaaacgc ttcttgctat tattattttt gaccccggtt caagaacgct
     4981 gccttcggga aaatcattaa gttctttcaa gtagcttatg gacagattat aattctttgc
     5041 aatatcccac aaggtttctt ccgactgtac aaccctgaat atcaagctgg gcattttgct
     5101 tctgtctata ggattatttt ctgcaatatc tttaattact gtaacttcat catcattttt
     5161 gaatttcatt cctacattta tgtcgcagtt aaaattcacc gagctgtttc cttttctgta
     5221 tgcactgcat ttatgaactg atactttaga atgtatgttg cttaatgctt catcctcatc
     5281 tacgggaaag gcagcagtga aggggaggga agcaaatgct ttgtctattt tatttatgtc
     5341 tccgtttaaa tacagcaagt ttacatccaa aaatccatct ataatgtatt tgttgtcagc
     5401 aatcctcttt tcggaaacct tgggacttgc atccacagaa taaatatcct ttattgagcc
     5461 tgaaaccaaa tcaaagttat tctcatattt gacaacttca ttggttaaat tcttcaggga
     5521 tgatagtttt actgtttcag actggaggtc cagaccgtat ttggttgaat agcagtccat
     5581 aattatattt ttctccattg ttttataaaa ggatgcattt acagtaaagg gcagggcaaa
     5641 ttctatcagt tttttctcat catcaaagtt ttccgttatt gaatatgtca tgtcatttac
     5701 gtttacacct atgtccttta gcatgttttc attggaaaat tccgattcca ggtagtgggt
     5761 aaagggaaat tcctccgata catatccgat tgtatgcatc tcatcgtctt caatgaacat
     5821 gaatccaacc ttgcatattc cttcaataag catcttatca tttaattcat ctatgtttgt
     5881 tatgtaaaca tcagcctctg acctgataac ctgagcaatt tcaccggaac ccttgctaag
     5941 ctccactgca tcattaatgt ttatttcttc agatatttct gctgctatat ccgtataggc
     6001 tatatttctg cttttggctt gaatggtccc gtcagattcc agattagaaa catatgcaac
     6061 cggtcttttc atataggcat cagtatccag tattaatact gccttaacaa gaaagctcct
     6121 ttctgtcagc tggtctgcat caatataatc aataaagaca tttgtaaaag cttccatgtc
     6181 ttccgtaacg ccttctgcgc tgatttcttc cctgaagggt atttcgcctg tcatggaatg
     6241 aatagtgtaa ttttcgttgt ttgaccggta aataatatta tatttaagcc ttccgtcaac
     6301 tattatacta tctgtggttg ccctaacatt atcgagcttt gcttttccct ccgcatttac
     6361 aattttctcc atatcaggca tgtcatcagc aattgtaatt tcattttcca gcaaaacttc
     6421 agtggaaaac gaatcgacaa cttcattaat attaaatgta tcattaaata atttaaccaa
     6481 aataaatcct cctttaagtt aatacggaat tacgcattat tcttttataa atgtatatgc
     6541 cgtttaaatt tcaatagtac aacttataaa caaaattttt tgaaaaagta ttaaaaggga
     6601 ttgacctttt cattttttta atatagaata attcagtaat gtggcagcgg attttgattg
     6661 aatttgccaa atttattaaa aacccaaaaa gatattgtat tgctggagac taaaaaaaaa
     6721 accctttttg tctccttttt atgttaaggg gtatgggtac acaggaaaca atgtacggag
     6781 acacacctga gacagagggt gagcctttac tggcacgagg aatgcccata attttgggca
     6841 ataacaaata gataaatcag gagggagaaa tgccaaaata tatttttgta accggaggag
     6901 ttgtttcttc attagggaaa ggaattactg cagcatcctt gggaaggctt ttgaaaagca
     6961 gagggcttaa ggttacattg cagaaatttg acccttattt aaatgtggat cctgggatga
     7021 tgagccctta tcagcatgga gaagtgtatg tgaccgacga tggagctgaa acagacttgg
     7081 acttggggca ttatgaaaga atagttgata tagatttaac ccagtacagc agcattacat
     7141 caggtaaaat ttactggtcc gtattgaata aggaaagaag aggagattac catgggggaa
     7201 ctgtccaggt tattcctcat gtaaccaacg aaatcaaaag aatattaaaa agaattgcca
     7261 cagtggaaga cgttgatatt gttataaccg aaatcggggg aacagtaggg gatattgaaa
     7321 gccagccatt tttggaggcc attcgtcaaa tacgctatga ggttggaaga gacaatgtat
     7381 tgtatataca tgttacatta cttccttatc tttcaatggc aggggagctg aagacaaaac
     7441 ccacccagca cagtgtaaag gagcttttaa gcataggaat tcagccggat atactggtat
     7501 gcagaagcga aaaagaaata tccgcagagc tgaaaacaaa aattgccctg ttctgcaatg
     7561 ttgaaaaaca aaatgttatt caaaatgtag acgcagattc catatatgat attccactga
     7621 tgctggaaaa ggaaggcctt gcaaaattag tatgcgacca tttcaactta aattgtgccg
     7681 aacctgattt ggcagaatgg acccaaatgg tagaccgttc aaagagccct aaacacagcg
     7741 ttaaaatagg tcttgtgggc aagtatgtag agcttaagga tgcatatctg tctgttgcag
     7801 aagctcttta tcatgcagga atcgataatt cctgtgatgt tcatatatca tggatacatt
     7861 cagagcaggt caatgaaaat aactataaac aaatacttga ggactgcgac gggattcttg
     7921 ttccgggagg ctttggagac agaggaatag aaggcaaaat cctggcagca aaatatgcaa
     7981 gagaaaataa aaagccatat ttgggaatag gactggggat gcagatggct cttgtggaat
     8041 ttgccaggtc agttttgggc tattctgacg ctgacagctt tgaattcaat cctgaatcca
     8101 aatatcttat catcaacaag gctgacggac ataatgacat agaaagcaac ggcggttaca
     8161 tgaggcttgg aagctatccc tgcaggctta ttgacggcac aaaggtaaag ggagcatatg
     8221 gaggggctga tatgattaat gaaagacacc gccacaggta tgagtttaat actgaattca
     8281 gagatgtatg cgagaagaat ggacttataa tttcaggggt ttcacctgac aacaagctgg
     8341 ttgaaataat agagctgaaa aatcatcctt ggtttgtggg agcccagttc catcctgaat
     8401 tcaaatcaag acctaccaga agtcatcctt tgtttaaata tttgataaaa gcttccatag
     8461 agtgtaaata gtatattgaa acagtgaaac agcggggacg gttctaaacc gtcccgcttt
     8521 tgtatcataa atacaactct cctaatcttt ttgatagatc gggacagtta agaaccgtcc
     8581 ccggccggct tttcctgtga gaaatatgtt cccagataaa tgcctccgat tattattata
     8641 ccgcccagca ctgtatatgc ggatggtatt tctttaaata taatcatagc cataaggctg
     8701 gcaaaaaccg gctccattaa tgttgttatg gaaatcaagg tggatgaata gtatttcatt
     8761 aaataattgt aaactgtatg ccccagtatt gaactgaaaa aggctaggag aaaaaacagg
     8821 gcaaactctt tcaaagggta gggatacaca ggtgttcccg atgcaaagca catgaaaaac
     8881 aatgctatag ccgataccga ataaacaatc aaaacatata ctcccgcact tacagtcttt
     8941 ctgacgatac ctcctattac cagataacct gcaacaaata ccgctcccat aaatgccagt
     9001 acattcccaa gaatcatggc tgtcccgttt ccctgagatg aacctatacc gattattaca
     9061 gtgcctatca atgacattcc aataccgaca atcattttac cggttattct ttccttcaat
     9121 agaaaatagt tgattgctgc aacaaagata gggctgcagg atacaaggat ggttgaattt
     9181 gcaattgtag tcatttgaat tgactgaatc caggaagcaa agtgaagtgc aagaaaaata
     9241 ccgcttatca ggcacagcag gcagtcattt ttatttaagc ttttaaattc gcttccgtat
     9301 tttatgtaaa caggaaagaa gagcatgatg gaagtaaaca gcatcctgta tgctgcaata
     9361 accagtgaag gtgattccga aaaccttatt attatcgaag aaacagaagt aaaaaaagtt
     9421 cctgcaagaa tccagtactt ggatggtttt ttcataattt cttcctatta tgttgataat
     9481 aagtatacta ccacaaattt taaatatttt caatattatg aagatcacaa tatatttata
     9541 cttatcagtc tttgttcata taataataaa aaagggttgt ggttgcggga gggcagagtg
     9601 gaattattta actccattac attgatttat gcagttaata tttgtatggc aataattgtt
     9661 atattcctgg aaagaaaaga ccctacggca acacttgcat gggtattggt cctcttgctg
     9721 tttcctggat tcggattttt gctgtacctg ttattagccc agaatttcag caggaagcag
     9781 ctttttatca tgaaaattta cgccaaaaaa tcatttggcg actatataag ggtgcaaaga
     9841 gaattgttcg ccactggggg gcttgttttt aatgataaaa atgttgaatc ctacaaggat
     9901 ttaattaaga tgaacttatt ttaccacggt ttttcataca cacaaaacaa tgaggtcaca
     9961 atctacacag acggtgaaaa aaagtttgat gagcttttca ggttaataaa agaggcaaag
    10021 agtcacatac atatggagta ttatataatg aggaatgaca ctcttgggaa caatcttttg
    10081 aaccttcttt caaaaaaggc gcaggagggt gtggaggtaa gacttttgta tgattccgtg
    10141 ggcggaaggc agataaccgg ggagcaaatt gaaaatttaa ggaggtccgg agttaaggtt
    10201 gctgtatttt ttgccagcac tattcccttc tttaatttta aaatcaatta cagaaatcac
    10261 aggaagatag tagttattga cgggataaca ggctttgtgg gcggtttcaa cgtgggggat
    10321 gagtatgtgg gcttaaataa taaaattggt tattggagag atactcattt gaaaatcaga
    10381 ggagatgcag taatagacct gcagaccagg tttttcctgg attggggtca tgcttccaag
    10441 gaagaggtct tgtttttgcc taaatatttt cctgacaaca gaatccaggg cagggtggga
    10501 attcaaatta taagcagcgg tcctgacagt ttagatgaag caatcaaaag caattatgta
    10561 aaaatgataa attcagctaa aaaaagcatt ttgattcaaa ccccatactt tgttcctgat
    10621 gcttccgtat ttgaagctat caaaattgct gccgcatccg gtgtggatgt gaaaattatg
    10681 attccctgca agcctgacca tccttttgtt tactgggcaa cctactggta ttgcggaggc
    10741 ctgttaaaat atggagtgaa ggtttatact tatgagtatg gattcttgca ttccaagacc
    10801 ctggtggttg acggcactgt ggcatctgta gggacagcaa acttcgatgt aagaagcttt
    10861 aaattgaatt ttgaatgcaa cggaataatt tatgaccggg acacatccca aatgctttat
    10921 gacatattta tggaagattt gaattattct atggagctga caagggaatt gtatctggaa
    10981 agggggattt taataagatt caaggaatcc gtgtcaaggc ttttagctcc tttgttataa
    11041 ggagcaggat attttcatct tggcagttga aattttttac agcaatgcta taattatcat
    11101 aatgaaaagt gcgggtgata aatatgaaaa gatattttgt tatatacaat ccatcttcgg
    11161 gaaaagagct tgcagcctat aaggtattcg gcgctgctga aacccttatg agtgttgaag
    11221 atgcggagtt ttcattctat tccacaaaga aaaagggaga tggggaagaa gctgcaataa
    11281 gagcatgccg gcttgattat gaaatgatta taacctgtgg cggcgacgga acagtaaatg
    11341 aagttgtcaa cggaatgatg aagtgttcca acaggcctaa attggcaata atgcctgccg
    11401 gaacagtgaa tgactttgcc gaacagctta accttcccaa ggctcaggag aattttgcct
    11461 ctcttatcat gcagaataaa tacagtacaa ttgatgtggg aaaaataaat gaaaaatatt
    11521 ttataaatgt agtgggaggg ggagctttta caaccattcc ccacagggtt acaattgatg
    11581 caaagacaat tctgggaaag tatgcctact actatcaggc agcggttaag gttcccgatg
    11641 agcttgagat ttgcgaagaa attgaatata taattgacgg aaaaaaactc ggttacaata
    11701 ctttgctttt tcttgtactt aactctgccg gcgcaggagg gttcaagtat ttgtgtccaa
    11761 aggcaaaatt ggacgacggc ctgcttgata ttgtaatttt cgaaaaaaca aattatgccg
    11821 agatgtttca gatatttaca aaagtgttta acggacatca cataactcat ccaaaggttc
    11881 attattttca ggcagagaat ataaaaataa ttcctgaaaa ggaattattg attgatgcag
    11941 atggagatcc ggcaggttat gcaccagcag aagtttcctc cgtgcacaat gccatagaca
    12001 tattggtgcc atagaaaatc ggcttatcct gtcagtatac cttcagaaag cggttgatta
    12061 atttaattgt cttatctatt gcatttttaa caaatatatc aaagtccatg tttgcagtgc
    12121 tgtcagcttt gtcggacatg gctctcacta tgcagaaatc cacattattc atatagcata
    12181 catgccctat tgctgcacct tccatttcgg tacaaagagc atcaaagtta tttactatat
    12241 aatccttttt ttctttgctt cctacaaact gatctcctga tgcaacggtt cccacggcta
    12301 tatttgtatc ttccaggatt tcggctgcct tcaccagttt ttttaccagt ctgtcactgc
    12361 agggaatttt aattaaatca attcctgaaa ttaaaccaac cgggactcca aaaggcgtaa
    12421 catcaaaatc atgctgtacc acactgtcgg atatgacaat gtctgcaaag tccaattccg
    12481 ggcttaagca tcctgcaaca ccgatattta ttatggcatc cggagcatat ttcaatatca
    12541 ttgtttgggt gcagacagcc gcatgtacct tgcctatacc gcaaattgca agcactgcat
    12601 ttttattctg cagttttcct tcgaagaaat ctatgccgct tatggtttct ttttttatta
    12661 aatccatgga atttataagg tcttcaacct caatgcccat tgcaccgatt attcctatca
    12721 taatatcgcc tccagagtta attaactaat tttaatatac caaaaaacaa atgtcaagaa
    12781 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcctccag agttaattaa
    12901 ctaattttaa tataccaaaa aacaaatgtc aagaagacgg gcggtcgtcc ccccaatcca
    12961 atttttttct ttctatttca gtataattgt gctaaaatgg actttaggcg gagcaaggat
    13021 atcttccaac ggcatatccc tgctgcccag ttgaaagggt gcttaaactg catgacaatt
    13081 tcttccgatt tttataaata aaaggtgata ggatgcatta tatatacatt ttagagtgca
    13141 gggacaaaac tttgtacact ggatatacta acgatttgga taaaagaatc aaaacacata
    13201 atgagaaaaa gggagcaaag tatacaagag gaagaactcc cgttgtattg aaatactatg
    13261 aggaatttga caataaaatt gatgcaatga aaagagaatc ccagataaaa aaactgagac
    13321 gcgtaaaaaa actgatgttg ttcaaataaa taaaatataa ttgtataatg tatatatttt
    13381 tgttataatt atataaaaca aaagaagagg agagttcgtc atggaatcaa acttaatagt
    13441 agacagctgc acagacttta atgaggaaac attagacata cccagagttc ctttcagaat
    13501 tataattgat gatgaagaaa tcgtagatga agacactgat ataattgagc ttataagaaa
    13561 aatgaaaagc agccgcaatc aaattaaaac tgcttgtgct cctccggaag acttcataaa
    13621 caaaatgaag gttcaagcaa ataattttat tgtaacgata tcaagccagt taagcggttc
    13681 ttacaacagc gccatggttg ccatggaaac cataaaggaa aagtttcctg aaacttttgt
    13741 acatgttttt gattccaagt cagctactgc aggggaaact ttgattgcac taaaagttaa
    13801 acaattgatt gaagaaaact taaatcccca tgaaatagtt gagcaggtag aaaaatacat
    13861 tcctaatatg agaacgctct ttatattgga ctcattggat aaccttgcca agaacggcag
    13921 gataagcaac tttaaggcac tccttgccaa cataatgaac attgttccaa tcatggggga
    13981 agacggggaa ggcagaataa ttttaaaaga aaaagtaaga ggcaaaaaga aagcattctc
    14041 gaggcttgtt gaaatacttg atgaatataa tattgattat caaaatacag ttttaggtat
    14101 aactcatgta aactgccttg aaaaagctga aaatttaaga gatgccataa aggcaaaata
    14161 taattttaag gacataaaaa tatttaaccc tgcggggttg agcactgttt atgcctatga
    14221 cggaggaata gtaatagctt tttaagggca tattttatgt tagttccctg ttaaattggg
    14281 tataattatt ttataccgaa atggagggaa cgaaatgaaa tataaagaaa ttatttctga
    14341 aattaaatcc gtaagacttt acaaaaagga tcaagtggat acaaaattat ttgatgaatt
    14401 aaaacaatat tatgaacagg gcaaaagact ggttgatgat attgaaatgg aagtgcttag
    14461 aaaaaacaga gatgaagttt ttgaacagct taaagactct gcaggctata acaacagaat
    14521 gatcgaagct ccccattatt taattgtgtt gtccgaagaa aaggatcact acatagaaaa
    14581 cgcaggctac agagtacagg atattatgct taaggcctgg agcctgggtc ttgcttcctg
    14641 ctggataacc tttgaagacg gggaagcaat taagaaaaaa ctaggcatta atacagacaa
    14701 aaagcttgct gcccttatag ctctcggtta tgaagaccct cagaacaagg ttttgtatga
    14761 gaaaggcaaa tacaatactt catcaaatgt taaagtaatt gaggataata caagcgaaag
    14821 acttggcata agggacctgg ttttcaccaa ggaatggggc caaaaggcag atcccgatga
    14881 gctggtgaac tacggtctgc tggaagggtt cttttacggg atactggctc cttcatacaa
    14941 aaatcgtcag ccctggagat ttatcgttga caacgggacg gttgttctgg cattgaaaaa
    15001 agatatatat gtaacagaat atgaagaaaa aatcgatacg gcagttataa tgctgtattt
    15061 tgatgcaatt atagaaagta ccttgcatga tatctcatgg aagttcggaa agcctgaaaa
    15121 agactacaag gtgcccgatg actataaaat tgcagcttat tgtatcattt gatgaaaagg
    15181 gctatgccct ttttacaata tagcttatgc tcccctggag acgggctgac tattataaaa
    15241 taaaggagaa ttacatgaaa attattattc acaagaaata cttggacctt gtatctgaaa
    15301 agtttccgga tattgaattt tacactgaaa ttgatgattg tcccgaagca gaagcactta
    15361 taggagagcc tatggatttt gtaccggaaa agctcgataa gctgcctggt ttgagatgga
    15421 tacaatcctt cagggcaggc tacgacacat tggatttaga ctatatgatg aaaagaaata
    15481 ttaccttctg caatggaaag gatatttact caattcccat agcagaggat gttgtttgta
    15541 aaatacttat gcataataca aatgccatgg catacttaaa gagtcaaaaa gaccataagt
    15601 gggacttaag ccaaagaaga acagaatttc agggcaaaac tataggaata attggaacag
    15661 gttccattgc tacagaaatt gccaagagac tccagggttt cggagtcaga ataatcggat
    15721 acaagagaag cccggtatct tcaatggcat attttaacga gattctttca ggcaagaaag
    15781 gattggagta tttaatgtca gaaagtgatt atataatagt aacagttgac ttgaataagg
    15841 aaacctacca tatgattaat aaggatactc ttaaatatat gaaagaaagc gctgcaatta
    15901 tcaatatagc aagaggaagc ataattaatc agaaagactt gacacaagca ttgaggaata
    15961 aatcaatctc ttatgcagga ctggatgttt ttgaagtaga acccctgcct gaagacgatg
    16021 aactgtgggg tttggaaaat gtgtacatca cccctcacgc atcaggcatt gtaagagaaa
    16081 ataaaaagag gcttggagac cttgttgtat ctaatattca aagattcata gacggtgaaa
    16141 agctggcata tgctgtaaaa tagaaattca tgcgggggaa catgatggtt ttcccgcata
    16201 tttaaaaaag aaagaagcat catgaatact aacaaaaata ttttgaaaat tttatttttc
    16261 cccctgttaa tactttatta tgaaacaatc ctcaggattt ttctatttga tgcagtattt
    16321 aatatgggat acatctacat gtgtttgttt tcctttcccc tggggctgtt cttttatttg
    16381 ctttcaaaag cttttaatga gaaaacaaac aaaattcttt tctatgtcat tgtaatcttt
    16441 ttaactctct attactgtgt tcaaataatt tattacagaa tatttaatac ctttacttca
    16501 tggtattcta ttttagctgg aaccggtaag gtattatggt ttaaagatat aataatctcc
    16561 gtacttcttg ataatatttt tgaattaatc atagtatttc ttcctctcgt tctattgttg
    16621 atttttcaca ggaatctcca attcaacaaa attataaaaa ggtatgctgt taatattgcc
    16681 ttatcagctc ttgtaatgca gacagcaatt gtaattgcgg tcctgtcttc tgacacggga
    16741 gtactaagcc cctcttattt atattccgaa acctttttgg tagtggaatc cgtagataag
    16801 tttggtcttt taacaacagg aagattagac atacaaaata ttttaaagag cctagtcttt
    16861 gctatagaaa tcaagcctcc ggaaataatt gacgagccgt ctgctgagcc gcaagatgaa
    16921 gatataccaa agcccccgca ggaagacccg tctgccggga ttgcagaata caatactatg
    16981 gatattcctt ttgacgattt gataaatacg gaaaaaaatc aggcaataag aaatatgcat
    17041 gaatacttca gcagtgttca gcccacaaag aaaaatgagt acacgggcag atacgaaggg
    17101 aaaaatttga ttctaataac tgcggagggt ttttcaccct atgctgtcag gaaagatata
    17161 acccctacct tatacaaaat gcaggaggag ggttttaagt tcaaaaattt ttacactcct
    17221 acctggggag tcagcacctc cgacggagaa tacgttgctt gtacggggct tttgcccaag
    17281 gaaggcatat ggagtttcta caaatcatct gcaaacttta tgccccttgc tatgggaaat
    17341 caactcaaaa aagaaggtta tgaaacaaaa gcctaccata accattactt tgattattat
    17401 tacagaaaca tgtcccatcc aaatctgggc tatatataca agggactggg caacgggctt
    17461 gatataacgg aaacatggcc tgaatccgat ttggaaatga ttgagcttac agtgccggaa
    17521 ttcatagaga atgagccctt ccatacctac tatatgacag tgagcggtca tttgcagtat
    17581 acatttacag gcaactacat agctgcaaaa aatagaagtc ttgtggaagc cctgccattc
    17641 tccaacaatg tcaaggcata tctggcatgc aacattgagc ttgacaaggc aatggagaga
    17701 ttaatacagg agctggagaa aaaagggatt gccgaagaca ctctaatagc cataagtccc
    17761 gaccactatc cctatggctt gaccctggcg gaaatcaatg agcttgcaga cagtgaaatc
    17821 aagggggact atgatttgga aaaaggggtg tttctccttt ggagcaaggg aatggagcct
    17881 gttgaaattg ataaaccatg ttcaagcctg gatataattc ctaccttgtc aaatctcatg
    17941 ggcttggatt atgattcaag gcttctgatg ggcagagata ttctttcaga ctcagaagct
    18001 ttagtgatgt tttcagacag aagctttatg acagacaagg tgttttacaa tgccagaacg
    18061 aatgaggtaa aagcattaac cggtgaagac attgaagatt catacattaa agaaagagca
    18121 gatacagttt tatcaaagtt caaatactca gctctgatac ttgacagtga ctactacaga
    18181 agaattttag attaaataaa atatttaggg acatttctct attgatattc cgaataaagt
    18241 ttctatcaat agggatgtgt ccccatacct ttgacggtgt ataccagtgt tctttaaaca
    18301 agtttatcaa tccacatcaa gaacatgctt cagacttacc gggcattggg tgtaggcaga
    18361 attatttatg cagtttgaat tgagcttgtt tgtcaatttc ccttctgcat cttttttgac
    18421 ttttacctta tagccgattc tttttatctc tgaggggaga aggcttccca catcccactt
    18481 gataatggtt tttccgttga attcataaac gtttttcggt tctaaattgc aagaatcccc
    18541 tatgtactca agtgccccgt caaggatgct tcttaaataa acctttcttg ccagatcata
    18601 ctgaccggtg gacaagttta catagtactt ggttatatcg cccggcttta gcctttcatc
    18661 attacattcc acactcatat caaaatccat tgcagttgag aaagcagaat atgaatcagt
    18721 aataccgcca ttgatcaaat tggcataaaa acttatttta cttctgtggg gtattttatc
    18781 ccctgaattt tcaatgctct cagctgtgta tttatcgcat agcgccgtgt caaatactat
    18841 tgtattagtt gaatttggag ataaagtaaa gttgtttcca aaaataatca cattatcgtt
    18901 attcaattct ttacagggct caacgatact gacattatcc agaatttctg tatccgggcc
    18961 ggcatggttc aggttatcca gatatcttat gccatttgcc aatgcagcac tgaaagaaca
    19021 aagtttttca atgtcagccg tatttttcag aattattttt atttgataaa tatatgatgg
    19081 aataaactga gaatttaaaa tacctgcacc ctttaaatat ttttcgggta catctatgta
    19141 tatggtgcat tctttcatta atttcattcc tttctgatgc aatataattc atattattct
    19201 cagctggggt ttttgtgaaa aatttcagct aaatatttat ggcataaaat aagctaatat
    19261 gataaaatta ttgatatata gaaaatgtaa tatcaataac ggaggataaa atgaaaaata
    19321 ttaaggttta ttccagctca acatgtacga attgtacggc tgcaaaggaa tacataaagg
    19381 aaaaaggtta taaatacgag gaaaagaatg tttcaacaga catggaagca agaaaagagc
    19441 tgatcgacat gggatatatg ggagtccctg taattatgat tgatgacgaa gtagttgtag
    19501 gatttaataa aggcaaactg gacgaaatat tatagtaaaa aaccggcttc gtgccggttt
    19561 ttgtaacatt tggtcaattc agtactatag cagaagacac tttgctaaat cttatttatt
    19621 caacgcagcc aacaaattgt ttaaattcat tccgtgtact gctgcagctt cttccaatgt
    19681 ttccatctga gcactggggc agcccacaca tcccattcca aatgccatta atatttcggc
    19741 tgcttcatgt tttgccataa taacttctcg aattgtcata tcttttgtaa tcataatact
    19801 actccttttg ttatataatt aaatactaaa tattataatt cattatacag caatgaaaat
    19861 aatattcaat atataattaa gtactttata agagaacgca gcggaaaatc ttataagctt
    19921 ttttaggctg tttttattct gcggatggca atctaccgaa agcagacaac atataaatta
    19981 ataacagctg cgtgaaattt aaaaatagaa acgtattccc ccatcatccg gctaaaaact
    20041 gtttgaatca cattataatg tatgatataa tcaaaaaggt taaatcagta ccaaagcaag
    20101 ggcaggagta ataatatgca aaaaataagc gacatgaaaa aatgtatcca aaagaaaagt
    20161 aaattaaata tgctgttaaa gcagacagaa caggatatta taaaagaaaa acttttgatt
    20221 aacaagctct ctgcggaatt ggagaaggaa agtcaggaca ttttaaatct aaaatccaat
    20281 aatattattt cattgtttca gaccgtatta gatacaaagg aagataaata caacaaggaa
    20341 aagcgggatc tgctaaaagc aaggttaaaa tatgaccaat gcaagaataa tgttaattat
    20401 ctggttgaag aaacaaagaa gattgtagat tatatagcag agttaaacgg ctgcgatgct
    20461 gaatatgaag aattaataaa caaaaagttg gaaaccataa atatggctga tgatgaaacc
    20521 ggtctggaat taaagaggct gataaaacgg aaagaaaata taaatgccaa tatggttgaa
    20581 atatatgaag caatatgtta cggggaaaaa gcattaaaag ctatagaatt agccataaaa
    20641 gaattagaag atgcagaaga cctggaaaac atagatgaaa gaggtaatat agataatgcc
    20701 aggtaccatg cggaacaaac tcaaagaatg ctttcaatgt tcaaaaaaga actatctgat
    20761 ataacaatgt ttacaggtac tgaaatagca gccgggacat ttgaaaattt ttcggattgt
    20821 ttctttgaca gcttaatttt tgactgggca gttcaatcag gcatatgcaa atcattagat
    20881 actgtaaaaa acacaaaaaa tcaattggat aaggcaatat caaagctgta tgaagaaaag
    20941 gtaacagaag aatttatggc aaagcagata gaagagcaaa taaaccaaat tatagataaa
    21001 gcataaaatt agacacttga ccttctcctg gagaaggttt ttttacgaac ggataatgca
    21061 attattagga ggaaaaaatg tcagtagaaa aagtaagaga ttatttaaaa caatttaaca
    21121 agggaaatga catactggaa atggacgaat caacagcaac cgtggagctg gctgcacttg
    21181 ctctggatac agaggaggca agaattgcaa aatcattatc attttatgac ggtgacggtg
    21241 caatggttat agtaacagca ggagatgcaa aaatagacaa caggaaattt aaagactact
    21301 ttggttttaa ggcaagaatg cttgcacctg aagatacaga aaaattcacc ggccatgcaa
    21361 tcggaggggt atgtcccttt gcactgccca aaaatgtaaa agtatacttg gatgaatcta
    21421 tgaaaagatt tgagacaatg tttccagcct gcggaacagg aaattctgca atagagctga
    21481 ctcttgagga gctggagcaa acatcacaaa gcaatacctg gatagatgtg tgcaaaaact
    21541 ggtgatgacc taaccccaca tgttcgagcc atagctaaga gcagatgacg ttaggtcata
    21601 gcaacaagtt ccgaatttat gctgcccaag gagcaaatat atgtgaacgg ggctgccgga
    21661 agcaggaaag gatattatgt caaacgaatt ttcaagaaca cagcttttat taggcaaaga
    21721 agcaatggaa aagctta
//
LOCUS       DAOPUT010000016        21250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_2471_length_21250_cov_20.176457, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000016 DAOPUT010000000
VERSION     DAOPUT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2471_length_21250_cov_20.176457"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..3749)
                     /locus_tag="PKK75_02975"
     CDS             complement(300..3749)
                     /locus_tag="PKK75_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ82588.1"
                     /translation="MALVKLNINGKEITAEAGKTVLDAALENGIEIPHLCFDERLEVY
                     AGCGLCIVEIEGQPKIARACATPVSNGLVVRSDTEKVKEARNNVLNLLVSQHIGDCKP
                     PCTLNCPANTDVQGYVGLVANGQYLESLKLIKEKLPLPACIGRVCPHPCEKACRRQHV
                     EDPVSIACIKTFAADYDLNSGSPYIPKLKPATGKKVAVVGAGPAGLSAAYYLLADGHQ
                     VDIFEAMPKPGGMLRYGIPEYRLPKNVVDAEVAIIEEMGANINYGVKVGEDMSLEYLK
                     DKYDAVFLGIGAWKSSPMRCEGEDTPGVLGGIDFLIKVAENKPVKVGRKVIVVGGGNT
                     AMDVARTSIRLGAEEVRVVYRRSEEQMPAEEIEIKEAKEEGIIFSFLSAPSLVLSDGE
                     KVTGLKCEKMVLGEKDASGRQSPVPTGEFVEFEADTIVAAIGQEVDCGKINVGQGRKK
                     NIVINEGTFETNIKGVFAGGDAATGPKIAIDAIAQGGRAAGVISSYLAGEMIPYKNLP
                     LVYTEVTDEDYVDEERLPRVPHRTVEAEVRKHNFQPILPTMTEEEVLREGKRCLECGC
                     KDYFECQLVNYIKKYEVDTEKYHATNEKKFKETDHPFISQNVDKCILCGLCVRTCSEV
                     VGASALGLVERGNETFVAPAFQQELKATSCISCGQCIDVCPTGAWLDRRENMKEIPLN
                     LSQTKSVCSYCSVGCEVVYEHKDNMVYLASSKENEIPACGKGKFGIEHINNKNRLLQP
                     KVKVKGVHEPVALDTAIYETAKRLRNIQNMYGDDQIAFVVSPKLTNEEFKYIKAVGDE
                     LNSKYKGSFTVDSESGIEYVLGRNQSTITAEELDNADLIISAGQLYEHHPAAGMKLRR
                     LSNTKKIISASTIKTKLDIFAEKAGVTEYEVFFTRVLKALVDNGVIKKESINRYENGE
                     ELIKVLDKIEVLPEAAKVAEAFKKARKPIIVADENTVPKAALKVLADITVLVGNDKRP
                     HRGLIVLKAKSNSQGAWNIGFRTPGEEIKKALLNNELKGLVVIGEDVLGNIPELKKAI
                     DNLKAFAVFDIMENETTAKADYLLPLCSIAESNGTIVSADGTVRNVVQAIKPLTGMSN
                     LEMFAKLAGLLNAVYKEDPKDEAKIKLYVPSLNSREKEYQVFDTVEKAYLAKLKENCV
                     KAV"
     gene            complement(3765..5567)
                     /locus_tag="PKK75_02980"
     CDS             complement(3765..5567)
                     /locus_tag="PKK75_02980"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNZ82589.1"
                     /translation="MAIFRSHVLICGGTGCTSNKSQLVRKRFEEKIKEVELDKEVQVV
                     GTGCFGLCEEGPIVIVYPEGSFYSKMTVEKVDEIVDEHLLKGRIVEKYLFKEKAKVEH
                     AEEHKSLMEVQFYSKQKRVALRNCGLINPEDINEYIARDGYTALGKVLTEMTPEDVIQ
                     LMKEANLRGRGGAGFPAGVKWSFAAPNKADQKYIICNADEGDPGAFMDRSVLEGDPHA
                     VLEAMAIAGYAIGATKGFIYVRAEYPIAVRRLHIAIGQARELGLLGKNIFDSGFDFDI
                     ETRLGAGAFVCGEETALMQSIQGNRGMAKIKPPFPANQGLWKKPTVINNVETLANVPQ
                     IILNGPEWFKSFGTAKSPGTKIFALGGKITNTGLVEVPMGITLREVIYDIGGGCPNGK
                     AFKAVQTGGPSGGCLTAGHLDTPVDYENLAAVGSIMGSGGMIVMDEDNCMVDIARFFL
                     DFIVDESCGKCTPCRVGTKRMLTILEKITEGKGTLEDLDELESLANSIKDSSLCALGQ
                     TAPNPVLATLRYFRDEYIAHVVDKKCPAKHCKALLTYEIDKDKCTGCTLCARKCPVSC
                     ISGTVKNPHEINKELCIKCGNCYSVCRFGAVQKN"
     gene            complement(5591..5956)
                     /locus_tag="PKK75_02985"
     CDS             complement(5591..5956)
                     /locus_tag="PKK75_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNZ82590.1"
                     /translation="MKSLEELKRIREEAIKNVEMRNTGKDIRVVVGMATCGISAGARP
                     VLATFVEEVAKRNLNNVQVVQTGCIGMCTYEPIVEIYAHDKKKVTYIHVDSEKAKRIV
                     AEHLVNGSVVTEYTIGSKI"
     gene            complement(5966..6448)
                     /locus_tag="PKK75_02990"
     CDS             complement(5966..6448)
                     /locus_tag="PKK75_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNZ82591.1"
                     /translation="MQNEFVLSKKSEFAELKEYIDSVKNSQGVTMQILQKAQGIFGYL
                     PLEVQQFISDETNIPLADIYGVVTFYTQFSTEEKGKHRIGLCLGTACYVKGSQEILDK
                     LADELDVKVGKTTKDKLFTLEATRCLGCCGLAPVMVIDDDVYGKLETSKISGILKKYQ
                     "
     gene            complement(6638..7096)
                     /locus_tag="PKK75_02995"
     CDS             complement(6638..7096)
                     /locus_tag="PKK75_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82592.1"
                     /translation="MKKRFIFTMAVLLLFSYTSISGYEYHKETDSIEYLLNERINIMN
                     EFLYGYKDMDDLEAKLCKIETGSLLQNDLAILHKVMDSPTDYELAINAKVDKIYEAVK
                     EDEEYSINADLKWLMRGYDGEFNLIRNYSIKCIDMDEEFYLTELTLKDRE"
     gene            complement(7217..8215)
                     /locus_tag="PKK75_03000"
     CDS             complement(7217..8215)
                     /locus_tag="PKK75_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ82593.1"
                     /translation="MITIKDVARLAEVSISTVSRVINDSKPVSPEVRKRVLKVIEETG
                     YKPNDVARSLVTRRSYLIGVIVNNLAYSYVADVVRGIEEIGKMYGYDILLCSSYGSKE
                     TQEKYLQLLDRKQAEGLFLVGNVFDEDIVKSAKSLKKPCVFFTRDIRENINHITVDCY
                     SAIYDVTKYLFEEGHRKIAYFSDFENRTTIENEKVEGYLKAVEDNETDYSKIYVVGGN
                     KHSKAYDFGTSIINEARDFTACVCSSDEIAIGILDSFLDNGINVPGDVSIVGFGNIRE
                     GKFVRPELTTIGEPYYDVGAVGMRVLIKMIKGDKTQKGRMELPFTIEKRKSVKSLN"
     gene            complement(8404..8578)
                     /gene="ssrS"
                     /locus_tag="PKK75_03005"
     ncRNA           complement(8404..8578)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKK75_03005"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(8690..10111)
                     /locus_tag="PKK75_03010"
     CDS             complement(8690..10111)
                     /locus_tag="PKK75_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ82594.1"
                     /translation="METYQIISIIGVLIFIYFIFFKNKRSAQKEINNLNEIEYTGSST
                     MTFDDVAGNSEAKESLLELVDFIQNPGKYSRFNVRMPKGILLYGEPGTGKTLMAKALA
                     GEAGVPFYAVSGSDFVQVYAGLGAGRIRNLFKKAKKSGKSVIFIDEIDAIGKTRAAGG
                     MKGSDEGDRTLNALLTEMSGFADDSGTLVVAATNRIDTLDKALLRPGRFDRQIEILLP
                     DKNARKDIINLYLKKIDAEDVNIDELADLTVYFSGAMIENLINEAALSAIRQGSDKIR
                     EEHIKRAYLEVLAGVEKKDYDNGRQKTVTSYHEAGHAFVSRYLLPENKIIRVSVIPTS
                     KGAGGYTLSSPCREEVTSKKKIINHLSVLMAGRASEEVFFGNENITAGAQNDIEKATS
                     LALDYVTRYGMNDSVFINYQLLSDKLRIPLTTVEESVKKLTSEIYGQVIKIIEDNKEK
                     VGKIARLLIEKEVICQDELDNLL"
     gene            complement(10292..10771)
                     /gene="ispF"
                     /locus_tag="PKK75_03015"
     CDS             complement(10292..10771)
                     /gene="ispF"
                     /locus_tag="PKK75_03015"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNZ82595.1"
                     /translation="MKVGIGYDVHALTENRKLIIGGVHIPYEKGLMGHSDADVLIHAI
                     MDSLLGAMGKGDIGRLFPDTDDRYKDADSRVLLRIVYELMRENKFAAGNIDAVVIAQN
                     PKLSPFIDEMRNNIACDLETDISNINIKATTTEYLGFEGRGEGIAAQCICLLETKEN"
     gene            complement(10782..11474)
                     /gene="ispD"
                     /locus_tag="PKK75_03020"
     CDS             complement(10782..11474)
                     /gene="ispD"
                     /locus_tag="PKK75_03020"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNZ82596.1"
                     /translation="MLNEKYVTAIIVAGGKGKRMGSEIPKQYMDIAGKTILDTTLYKF
                     EKSNEVDTIILVVNKEDMDYVKDEIAPCYHKVAHVVQGGKTRVESVYQGLKKAGESSG
                     IVLIHDGVRPFVSYNLISTCIENAYVHKACVPVIEVLDTIKEVSEDGMVVKTLDRKTL
                     RAVQTPQAFDYEIIRDCYEKAVTEDVQFTDDASIAEYYGYNVKTIEGLYKNIKITTPL
                     DLRLAEIIASIY"
     gene            complement(11518..>12111)
                     /locus_tag="PKK75_03025"
     CDS             complement(11518..>12111)
                     /locus_tag="PKK75_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82597.1"
                     /translation="YHLNLYNYDINDLRLLELLNWTNQNKLSSNFDYINYAANKYDNV
                     SAEDYERYEYDRKRGGWKSANYMIDKGIHKDIPEHLKDVYNSGLKAMLKSPEEVIRTA
                     KKSGGKIFLSHPSYHYRDTAMPPEELEYWLEAGIDGMECFSPYNGNKAQYYVDFCKRN
                     NLMISGGSDCHGDFIPSRLLGVPNLSLDDLNIKKLLD"
     assembly_gap    12112..12211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12212..12521)
                     /locus_tag="PKK75_03030"
     CDS             complement(<12212..12521)
                     /locus_tag="PKK75_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82598.1"
                     /translation="MIDFFLSYKAIMKKVDMHMHTMASDGTWGATELKNELIKNKIEI
                     FSVTDHDSVDNIKNMQDILRNRDNLIFIPGTELSAEYEGREYHLNLYNYDINDLRLLE
                     L"
     gene            complement(12533..13528)
                     /locus_tag="PKK75_03035"
     CDS             complement(12533..13528)
                     /locus_tag="PKK75_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNZ82599.1"
                     /translation="MKKLLMIATGGTIASINTKNGLAPEIGSEELISFVPEIRQYCHV
                     DTIQLINIDSTNIQPEHWVMMTEAIEENYHKYDGFVISHGTDTLAYTSAALSYLIQNP
                     NKPIVVTGAQKPINVEITDAKKNLLDSFRFASEDGVSGVYVVFDGKAIIGTRARKVKS
                     KSYSAFESINFPVAAIIDDRRITKYISNYNLEKEVKFYKDIYPNIFLLKLAPGMEADV
                     LDYIGKKYRGVVIESYGAGGLPFLDKHNFLNKLEGLIEKGKIVVVATQVMFEGSDMGI
                     YEVGVKALQKYNVLQAYDMTIEAVITKLMWIMAQTKNFEEVKAMFYTRINDDSLY"
     gene            complement(13586..14485)
                     /locus_tag="PKK75_03040"
     CDS             complement(13586..14485)
                     /locus_tag="PKK75_03040"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ82600.1"
                     /translation="MFNNILNYRIQENDKTVKTILTEKFKFSGRLSRKLERTGKILLN
                     GKAVKLSKSIFEGDELSIEFDEEEDQYAAVDIPAKIIYEDDDLLAVDKQPYVVVHPAR
                     SHQNNTLANGLAYYFKENNIHRKVRFVNRLDMNTSGIVIIAKNPYAHNQMAVQMKSNQ
                     VEKYYYAIVEGHMKEDRGRIDAPITRLNPEDIIRIVHPSGKECITEFKKEKIFNNMIL
                     VKLKLVTGRTHQIRVHLKYIGHPVIGDTLYGSESSLIGRQALHCYEMRFKHPVTNKEM
                     IITCPLPEDMEKIINGDDEFTNK"
     gene            14757..15560
                     /locus_tag="PKK75_03045"
     CDS             14757..15560
                     /locus_tag="PKK75_03045"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNZ82601.1"
                     /translation="MKEKVINIAANDDSYTSEIKRKLIRVLTENGYNYYEGFNEDAQL
                     NITVGGDGAFLRGVRASNFSQIPFIGVNTGTLGFYPEITPENLENFIINYRAGNYTIN
                     SVNLIECEIVTANLSNKIYAVNDIILKRKDMKTIHLNVFIASHHLEKISGDGLIISSP
                     LGSSAYNKSAGGSLVYPSLKTLQITPLSPILSNAYRCLECSIIVPPEFEIAIYPERRD
                     DYCLAILADGEVFEYDEMEHVLFRTSKRIINRLTTGTFNYWNVIKEKFL"
     gene            complement(15651..16913)
                     /locus_tag="PKK75_03050"
     CDS             complement(15651..16913)
                     /locus_tag="PKK75_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ82602.1"
                     /translation="MFRKLHLKLTAYMGIILILFMFFVSAGIYKFTKIIFEDGNKELM
                     RAEALRIYNYKNTAYADYFFNGGLFRRYINNPSDTKRLDACYIVYNQNLTPVLSDEGD
                     TEIADYIKPLAQEAIEEKEDSYTTKRIGRFNYRIYTRYFNDITGHSIVQVYQNTVNEE
                     ILWTFLRNVLTVIGICGTVVLLALSYILTGKALAPVRETWVRQREFVADASHELRTPL
                     TVIQTNLDAVLSDEDGTVEENEIWLDNAYSETRVMAKLIDQLLILAKADNNEEKLDIS
                     EFSFSEVVENVCQTMEVIASKKNIELITDIEDNIIIKADYDKMRRLAVILIDNAVKYT
                     DKGRVKVSLKTDKNKKFLIVEDTGIGISEKDLDRIFDRFYRADKARHREGGTGLGLSI
                     AKWIADKHKYSLTAESTVNAGSKFTLKI"
     gene            complement(16914..17582)
                     /locus_tag="PKK75_03055"
     CDS             complement(16914..17582)
                     /locus_tag="PKK75_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ82603.1"
                     /translation="MRILIVEDEVKITQALNFLFNKQKIDVDIANDGEEGLILAGKDI
                     YDVIVLDIMLPGIDGIQILKTIRKEGVKTPVIMLTAKDTVDDRVFGLETGADDYLVKP
                     FATKELIARVKALARRKSTEFAGEVFEFEDIKYDVKNYLLYVGDTEYKIPLKEAMLME
                     MFIRKPRQVFTREQIIDRIWGLEADILESNIEIYVHHLRKRLEDTNVKIETVRGVGYM
                     LKEK"
     gene            complement(17877..19502)
                     /gene="groL"
                     /locus_tag="PKK75_03060"
     CDS             complement(17877..19502)
                     /gene="groL"
                     /locus_tag="PKK75_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833193.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNZ82604.1"
                     /translation="MAKIIKFDEDARRSMERGVNALADTVKVTLGPKGRNVVLHKSFG
                     SPQITNDGVTIAREIELSDPYENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIIREG
                     LKNVAAGANPMILKKGIEKAVSKAVDELKSISKKVETSEEIAQIASISAGDEEIGQLI
                     SQAMDDVGKDGVITVEESKTMGTELTVVEGMQFDRGYLSAYMVTNTEKMEAVLDNPYI
                     LITDKKITNIQEVLPILEQIVQTGKSLLIIAEDVEGEALATLVVNKLRGTFNCVAVKA
                     PGFGDRRKEMLRDIAILTGGQVISEELGLDLKETTLEMLGNAGTVKIDKENTVIVNGA
                     GAKEDIDERAKQIRAQHEESTSEFDKEKLMERLAKLTGGVAVINVGAATETEMKEKKL
                     RIEDALNATRAAVEEGLVPGGGVALLNTIGAVAGVAEGLEGDAKTGAKIIEKALEEPL
                     RQIVINAGLEGSVVVEKIKHSNKGVGFDAMTEKYVNMVESGIVDPTKVVRSALQNAAS
                     VAAMLLTTEAAVADEPKEEPAMPPMGGGGMGMM"
     gene            complement(19519..19800)
                     /locus_tag="PKK75_03065"
     CDS             complement(19519..19800)
                     /locus_tag="PKK75_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNZ82605.1"
                     /translation="MTIKPLGERVVIKMTEAEEKTKSGIVLPGSAKEKPQVAEVLAVG
                     AGITNDEKKKDEIKVGDKVIFSKFSGTEVKIDGEEVIIIKLADVLAVIE"
     gene            20166..21095
                     /gene="fba"
                     /locus_tag="PKK75_03070"
     CDS             20166..21095
                     /gene="fba"
                     /locus_tag="PKK75_03070"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNZ82606.1"
                     /translation="MALVTSQEMFKKAYEGNYAVGAFNVNNMEIIQGIVDAAKQENAP
                     LILQVSAGARKYANPIYLRKLVEAAIEDTGLDICLHLDHGEDFEICKQCVDDGFSSVM
                     IDGSKYPLEENIALTKRVVEYAHSKGVVVEGELGKLAGVEDAVKVKTKDATYTDPNEA
                     VEFVERTGVDSLAIAIGTSHGAYKFKGDPSLDFERLEIITNLLPGFPLVLHGSSSVPK
                     DFVDLCNKYGGQIPGAAGVPEEMLRRAAQMGVCKINIDTDLRLALTACIRQYFFEHPS
                     EFDPRKYLGPGRTAIKDMVAHKMRNVLGCSNKR"
BASE COUNT         5599 a         4613 c         3470 g         7468 t
ORIGIN      
        1 gtgtcaggaa tttcgaaaga ttcagaaaca ggatactgca tgtgatgagt tagccggccg
       61 gcacaagctc cgagccatgt caagtacacc cgcatgcggg tgtacttgac atgtttcaaa
      121 ataccattta aaactcaaaa tgggcggtta agctaaaatc cgctcaaacg cccatcttaa
      181 aaatcatttt tttgtgtctt tattcaatgt taggattttc taaccccaac aattgacaaa
      241 gagcctttta gataaacgat ttttaacaac taaatattaa tgttttctta agcattaggt
      301 catacggctt tcacgcagtt ttcttttagt tttgccaggt aagccttttc tactgtatcg
      361 aatacctggt attctttttc tctgctgttt aatgaaggta cataaagctt aatctttgcc
      421 tcatctttag gatcttcttt gtatactgca tttaacagcc cggctaattt agcaaacatt
      481 tcaaggtttg acatgcctgt taatggtttt attgcttgaa ctacatttct tacagtgcca
      541 tctgcgctta ctattgtacc gttgctttca gctatgctgc aaagcggaag cagataatcg
      601 gctttggctg tagtttcatt ttccattatg tcaaacactg caaaggcttt aagattgtct
      661 attgctttct taagctcagg aatatttccc aatacatctt cgcctattac cactaaacct
      721 ttcagctcgt tgtttaacaa agctttcttg atttcttccc cgggagttct gaaaccaatg
      781 ttccaggctc cctggctgtt tgatttagct ttgagaacta tcaaacctct gtgggggcgt
      841 ttgtcgtttc ctactaaaac agttatgtca gccaatacct tcagtgctgc tttcggaact
      901 gtgttttcat ctgcaacaat gattggtttt cttgctttct tgaaggcttc cgcaacttta
      961 gccgcttcag gaagaacttc tattttatcc aataccttga ttaattcttc tccgttttca
     1021 tagcgattga ttgactcttt cttgataacc ccgttatcaa ccaatgcttt caaaacgcgt
     1081 gtaaagaata cttcatattc tgttacgcct gctttttctg caaaaatatc cagctttgtt
     1141 ttaattgttg aagcagaaat tattttcttg gtattggaca atcttctgag tttcattcct
     1201 gcagcaggat gatgttcata caactgtcct gctgaaatta tcaaatcagc attgtccagc
     1261 tcttcagctg ttatagttga ttgattcctg cctaatacat attcaatacc ggattctgaa
     1321 tcaaccgtaa atgaaccttt atatttacta tttaattcat ctcccacagc cttgatatat
     1381 ttgaattctt catttgtcag cttgggtgat actacaaatg ctatctgatc atcaccgtac
     1441 atattttgta tgtttcttaa gcgttttgct gtttcatata ttgctgtgtc aagagcaaca
     1501 ggttcgtgaa cccctttaac cttaaccttt ggctgaagga gtctgttttt attgttaatg
     1561 tgttcaatac cgaatttacc cttgccacag gcaggtattt cattttcttt gctggaagca
     1621 aggtatacca tattatcctt gtgctcatat acaacttcgc atcctaccga gcagtagctg
     1681 caaactgatt tggtttgtga aaggttcaga ggaatttcct tcatattttc ccttctgtca
     1741 agccaagctc ccgtaggaca tacgtcaata cactgaccgc aggaaataca gcttgttgcc
     1801 tttaattcct gctggaatgc aggagctaca aatgtttcat ttcctctttc aaccagtcct
     1861 aatgctgatg caccaaccac ttcgctgcat gtacgtacgc aaagtccgca tagtatgcat
     1921 ttgtcaacat tctgtgaaat aaagggatgg tctgtttctt tgaatttctt ttcattagtt
     1981 gcatgatatt tttcagtatc aacttcatac ttcttaatat aattaacaag ctggcattca
     2041 aagtaatctt tgcagccgca ttcaagacat ctttttcctt ctctcaatac ttcttcctca
     2101 gtcatagtgg gaagtatagg ctggaagtta tgctttctta cttcagcttc aacagtcctg
     2161 tgaggaactc ttggaagtct ttcttcatca acatagtctt catctgttac ttcagtatat
     2221 actaaaggca ggttcttgta aggaatcatt tctcctgcca agtagctgct tataactcct
     2281 gcagcccgtc caccctgtgc aatggcgtct atggcaatct tgggaccggt tgcggcatct
     2341 cctcctgcaa acacgccttt aatatttgtt tcaaaggttc cttcattgat tactatattt
     2401 ttctttcttc cttgtcctac gttaattttt ccgcagtcaa cttcctggcc tattgcagca
     2461 acaattgtat ctgcttcaaa ctctacaaac tcgccggttg gaacagggct ttgcctgcct
     2521 gatgcatcct tttcaccaag aaccatcttt tcgcatttaa gtcccgttac cttttcaccg
     2581 tcgcttagaa ctaatgaagg tgctgacaag aatgagaaaa ttattccttc ttcttttgct
     2641 tctttaattt ctatttcttc ggcaggcatt tgctcttcac ttcttctgta aacaactctt
     2701 acttcctctg ctcctaaacg tatgcttgtt cttgcaacgt ccatggcagt gtttccgccg
     2761 cctactacta ttactttcct gcctactttt acaggcttgt tttctgcaac cttgattaag
     2821 aaatcaattc cgcctaaaac tccgggagtg tcttctcctt cacatctcat aggcgagctt
     2881 ttccatgctc cgattccaag gaacactgca tcatatttgt ccttaaggta ctccagagac
     2941 atgtcttcgc cgactttcac cccgtaattt atgtttgcac ccatttcctc aatgattgca
     3001 acttcagcat caactacatt tttggggagt ctatattcgg gaataccata cctgagcatt
     3061 ccgccgggtt ttggcattgc ttcgaatata tccacctggt gaccgtctgc caagaggtaa
     3121 tacgctgcag acaatcctgc aggacctgct cctacgacag caaccttctt gcctgttgca
     3181 ggctttaatt ttggaatata ggggctgcct gaatttaaat catagtcggc tgcaaatgtc
     3241 ttaatgcatg ctattgaaac ggggtcttcc acatgctgtc tgcggcatgc tttctcgcaa
     3301 ggatgaggac aaactcttcc tatacaagca ggcagaggta atttttcctt tatcagtttt
     3361 aatgattcga gatattgacc attggctact aaaccaacat aaccttgtac atccgtattt
     3421 gccggacaat ttaatgtaca tggaggtttg cagtcgccta tatgctgaga aaccagcaaa
     3481 ttcagtacat tatttcttgc ttctttaact ttttcagtgt ctgatcttac aaccagtccg
     3541 tttgaaacgg gagttgcgca tgctcgtgca attttaggct gtccctcaat ttcaacaata
     3601 catagtccac accctgcata tacttccaat ctttcgtcaa aacacaagtg cggaatttct
     3661 atgccattct ctaaagctgc atcaagtact gtctttccag cttcagctgt gatttctttt
     3721 ccatttatat taagttttac taaggccacg gctaatcctc cttcttaatt tttttgtaca
     3781 gcgccgaatc tgcatacgct gtagcaattt ccgcatttaa tacataattc tttattgatt
     3841 tcatgaggat ttttaacagt tcctgaaata cagcttacag gacactttct tgcacaaaga
     3901 gtacaacctg tgcatttatc cttgtcaatt tcataagtta acaaagcttt acaatgcttt
     3961 gcaggacact ttttatctac aacatgtgca atatattcat ccctgaaata tctgagtgta
     4021 gccagtaccg ggttcggcgc tgtttgtcca agcgcacaca aggatgaatc cttaatgctg
     4081 tttgccaagc tttcaagttc gtccaaatct tctaatgtac cttttccttc agtgattttt
     4141 tcaagtatag tgagcattct ttttgttccc actctgcagg gtgtacattt accgcaggat
     4201 tcatcaacta taaaatctag gaagaacctt gcaatatcaa ccatgcagtt gtcttcgtcc
     4261 ataactatca ttccacctga gcccattatt gagcctacag cggcaagatt ttcataatct
     4321 acaggggtat caagatggcc ggctgtaaga catcctccgg aaggtcctcc tgtttgaact
     4381 gctttgaatg cttttccgtt aggacatccc ccgccaatgt catatattac ttcccttaat
     4441 gtgattccca tgggcacttc aacaagtcca gtgttcgtaa ttttcccgcc caatgcaaat
     4501 atttttgttc ctggtgattt tgcggtaccg aagcttttaa accattccgg cccgttcagt
     4561 ataatctgcg gtacatttgc caaggtctca acattgttta ttactgtcgg ttttttccat
     4621 aatccctggt tagccgggaa cggcggctta attttcgcca ttcctctgtt tccttgaatt
     4681 gactgcatca gtgccgtttc ttctccgcat acgaatgccc ctgcacccag tcttgtttct
     4741 atatcaaaat caaaacctga atcaaatata ttcttgccta aaagtccaag ctctcttgcc
     4801 tggccgatag ctatatgcaa tctgcgcact gctataggat attctgccct gacatatata
     4861 aatccttttg tggcacctat agcatagcct gctattgcca ttgcttccaa tactgcatga
     4921 gggtctcctt caagaacgct tctgtccatg aatgcaccgg ggtctccttc gtcagcgttg
     4981 catattatat acttctggtc tgctttattg ggtgctgcaa aactccactt aactcctgca
     5041 gggaatcctg ctccgcccct gccccttaag ttagcttcct tcattaattg tataacgtct
     5101 tcaggagtca tttcggtcaa tacttttccc aatgcagtat aaccgtctct tgcaatatat
     5161 tcattgatgt cttcaggatt tattaaaccg cagttcctga gggcaaccct tttttgcttg
     5221 ctgtaaaact gaacttccat caatgattta tgttcttccg catgttctac tttggctttt
     5281 tctttgaata aatatttttc tactattctt ccttttaaca agtgctcgtc aacaatttcg
     5341 tcaacctttt ccacagtcat ttttgaataa aatgagcctt caggatagac tattactatg
     5401 gggccttctt cacagagacc gaagcagccc gttcccacaa cctgtacctc tttgtctaat
     5461 tccacttctt ttattttctc ttcaaatctt tttctgacta attgagattt gttagaagta
     5521 catccggttc ccccgcatat caaaacatga gatctgaaaa tcgccatggt tttacctccg
     5581 tttcataatc ttaaattttt gatccaattg tatattctgt tacaacacta ccattaacca
     5641 ggtgttccgc aactattctt tttgcctttt cggagtcaac atgaatatat gttacctttt
     5701 tcttgtcatg ggcatatatt tctacgattg gttcgtatgt gcacatgcct atacagcctg
     5761 tctgaacaac ttgtacatta tttaaatttc tttttgccac ttcttctacg aatgtagcaa
     5821 gaacaggacg ggcgcctgct gatattccgc atgttgccat acctacaaca actcttatat
     5881 ctttgccggt atttcgcatt tcaacatttt ttattgcttc ttcccttatc ctttttaatt
     5941 cttccaatga cttcatggtg acctcctatt ggtatttttt tagaattcct gatatttttg
     6001 atgtttccaa tttgccgtaa acatcatcat caatgaccat aaccggggct aatccgcagc
     6061 agcctaagca tcgggttgct tccagggtaa aaagcttgtc cttggttgtc tttcccacct
     6121 ttacatccag ttcatcggca agtttgtcca aaatttcctg agaccccttt acatagcagg
     6181 ctgtaccaag gcacagtcca attctatgtt tgcctttttc ttctgttgaa aattgagtat
     6241 aaaatgttac taccccataa atatcagcca atggtatatt cgtttcatct gatatgaact
     6301 gttgaacttc aagaggcaaa tacccgaata ttccttgtgc cttctgcagt atctgcatgg
     6361 tcacaccttg tgaattcttt accgaatcga tgtactcttt taattcagca aattcacttt
     6421 tttttgatag tacaaattcg ttctgcaatt ctccaacctc catatttaaa tatataaaaa
     6481 tataaatata attatataac taataatcaa aaaagtcaaa aaaatgtatg tttatttatt
     6541 attctgttaa aattttctca tctcaagcaa aaaatcctct attgtagagg attgattaaa
     6601 atatcttaac atacatagta tatcgattat actaatgtca ttccctgtcc ttcagcgtta
     6661 attctgtcaa ataaaactct tcgtccatat ctatacattt aatgctgtaa tttcttatta
     6721 agttgaattc accgtcatag cccctcataa gccattttaa atcagcgttg atggaatatt
     6781 cttcatcctc ttttacagct tcgtaaattt tatcaacctt cgcatttatt gccagctcat
     6841 agtcagtggg gctgtccata accttgtgaa gaatagctaa atcattttgc agcagactgc
     6901 ctgtttcaat tttgcagagt tttgcttcca aatcgtccat atctttataa ccgtagagga
     6961 attcattcat aatatttatc cgctcattta acaaatattc aatggaatca gtttctttat
     7021 ggtattcata accgctgatt gaagtataac taaagagcaa gagtactgcc atggtgaaaa
     7081 taaacctttt tttcattata aatccccctt aataaagata gtctttttat tataggggga
     7141 ttgccataag aattttatca ataattgtca tgtgttaata caaattcttc tttcaatttc
     7201 aacaaagttt gcaaaatcaa ttcaagcttt ttacgctttt tcttttttct atagtaaatg
     7261 gcagctccat gcgccctttc tgagttttat ctccttttat catttttata agcaccctca
     7321 tgcctactgc tcccacatca taataaggtt cgcctatggt ggtcagctct ggcctaacga
     7381 attttccttc acggatattt ccaaatccca caatagatac atcccctgga acgtttattc
     7441 cattgtctaa aaaactgtca agtataccta ttgctatttc atcagaactg catacgcagg
     7501 cggtgaaatc ccttgcttca tttattatgg aagtcccaaa atcatatgct ttgctgtgct
     7561 tgtttcctcc gacaacataa attttagaat aatctgtttc gttatcctca actgctttta
     7621 aatacccttc aaccttttca ttttctattg tagttctgtt ttcaaagtct gaaaaatatg
     7681 caatttttct atggccttct tcaaataaat actttgtaac atcatatatc gcagaatagc
     7741 agtcaacagt tatatggttt atattttcac gaatatccct ggtaaagaat acacatggtt
     7801 ttttaaggct tttagctgat ttaactatat cttcatcaaa tacattccct acaagaaaca
     7861 aaccttccgc ctgctttctg tccaaaagct gcagatattt ttcctgtgtt tccttacttc
     7921 cgtagctgct gcaaagaaga atatcatatc cgtacatctt cccgatttct tctattcctc
     7981 taacgacatc ggcaacataa gagtaggcca ggttgttaac tataactcct atcaaatagg
     8041 acctccttgt tacaaggctc cttgcaacat catttggttt ataaccggtt tcttcaataa
     8101 cctttaatac tcttttcctg acttccgggc tgacaggttt tgagtcgttt attactcttg
     8161 atacggtcga tatggatact tctgcaagcc tggcaacgtc ttttattgta atcatgtttt
     8221 tcctcctgtt tttaacattg gtaatgtatt cctctttatt tttattatat atgtatttca
     8281 ttgcaaaggc aaatattatt ttcccgtttt cctgaatata tcttcgttaa aggtatgggg
     8341 acacacctcc ctatttaggg aaagtctcta ttggcaggag gaatgtcccc aaatttataa
     8401 aataaaaaac ccgcgtgagc cgcaaaccca taattcacac ccgcaccttg gcaggtggga
     8461 gtcctgttgc ctgattctgg cttcccgtca aagcgggctt gtcataggca ggcaaacttg
     8521 aattcccttt tacattatgt cggttgaatt aaaagatttc cgaacaccgc aggctgttta
     8581 tttaattata ttatatcaaa tttatgcaat atttacaact gatttttaca tgtaatatag
     8641 tatttaatga tattttatcc tgcgatttag ataatattca actatatttt tacagtaaat
     8701 tatccagttc atcctggcag ataacttctt tttcaattaa aagccttgct attttaccga
     8761 ctttttcttt gttgtcttct attattttaa taacctgtcc atatatttcc gaagtcagtt
     8821 tttttactga ctcctcaact gtggttaagg gtattctcag tttatctgac aacaattggt
     8881 aatttatgaa tacagaatca ttcataccgt atcttgtaac ataatcaaga gccagtgagg
     8941 ttgctttttc tatatcgttt tgcgctccgg cagttatatt ctcattaccg aaaaacactt
     9001 cttctgatgc tcttcctgcc atcaatactg aaagatgatt aattattttc tttttgcttg
     9061 taacttcttc ccggcaggga gagctgagtg tatatcctcc tgctcccttg cttgtgggaa
     9121 ttacagaaac acggataatt ttattttcag gaagcaggta tcttgaaaca aaggcatgtc
     9181 ccgcttcatg atatgaagtg actgtttttt gccttccgtt atcataatcc tttttttcaa
     9241 cccctgccaa aacttccaga tatgctcttt ttatatgttc ttcccttatt ttatccgacc
     9301 cttgccttat tgccgacagg gccgcttcat ttattaaatt ttcaatcatt gctccgctga
     9361 aataaacggt caaatcggct aattcatcaa tatttacatc ttctgcatct atttttttca
     9421 agtacaggtt tattatgtcc tttcttgcat ttttgtcagg cagcaatatt tctatctgcc
     9481 tgtcaaacct tcccggtctt aacaaggctt tgtccagagt atctattctg tttgtggctg
     9541 caaccaccag ggtccctgaa tcatctgcaa atccggacat ttcggttaaa agcgcattta
     9601 aggttctgtc tccttcatcg cttcctttca tgccgcctgc agctctggtc tttccaatgg
     9661 cgtctatctc atctatgaat ataacgcttt tacctgattt tttggccttt ttaaaaaggt
     9721 ttcttattct tcctgcacca aggccggcat aaacctgcac aaaatctgag cctgatacgg
     9781 cataaaaggg caccccggct tctcctgcca acgcttttgc catcaacgtc ttccctgttc
     9841 ctggttcgcc gtaaagaagg attccctttg gcatcctcac attaaacctg ctgtactttc
     9901 caggattttg tatgaagtct accagttcaa gaaggctttc tttagcttct gagtttcccg
     9961 ctacatcatc gaaggtcatg gttgatgagc ctgtatattc tatttcattt aaattgttga
    10021 tttctttttg ggctgatctt ttattcttaa aaaatatgaa atatataaaa atcagtacgc
    10081 ctataattga aataatttga taagtttcca tgcaatcccc cggcagcaat gtaagtatgt
    10141 gtacatttca gcacatggac actttcttat ggtctaatca taaggtgtgt cattcttccg
    10201 ctgaagatgt gtcccaaaca tttactttta ttagccaaat tatagcacag ggatatacat
    10261 ttatatatgc tcaatatata ggttgtattc attaattttc ctttgtttcc agcaaacata
    10321 tgcactgggc ggcaatgcct tcgccccttc cttcaaaacc taaatactca gtggtggttg
    10381 ccttgatgtt tatattggaa atatcagttt ccaggtcaca ggcgatatta ttcctcattt
    10441 catcaataaa gggagagagc tttggatttt gagcaattac aacagcatca atattgccgg
    10501 ccgcaaattt attttctctc attaattcat acactattct taataaaaca cggctgtctg
    10561 catccttata cctgtcatcc gtatccggaa acagtcttcc tatgtcgcct tttcccatgg
    10621 ctcccaaaag actgtccatt attgcatgaa ttaaaacatc tgcatctgag tggcccataa
    10681 gacctttttc ataaggaatg tgaactcccc ctatgatcag ctttctgttt tctgttaaag
    10741 catgtacatc gtaacctatt ccaactttca tgccggctcc tttaataaat gctggcaatg
    10801 atttctgcca gtcttaaatc cagtggtgtt gttatcttaa tattcttgta taacccttct
    10861 attgttttta cattgtaacc gtaatattcc gcaatggatg cgtcatcagt aaactgcaca
    10921 tcctcggtaa cggccttttc atagcaatcc cttattattt cataatcaaa agcctggggt
    10981 gtctgaacag cccttaatgt ttttctgtca agcgttttta caaccatgcc gtcttccgaa
    11041 acttccttga tggtgtccaa aacttctatc acaggcacac atgccttgtg aacatatgca
    11101 ttttctatac atgtgcttat cagattatag gagacaaagg gtcttacacc atcgtggatt
    11161 aagactatgc cggagctttc tccggccttt ttcaaacctt gataaaccga ttcaaccctt
    11221 gttttgccgc cctgcaccac atgtgcaacc ttatggtaac agggggcaat ttcatccttt
    11281 acataatcca tgtcctcctt gttgacaaca aggattatag tatctacttc attgcttttt
    11341 tcgaatttgt acaaggtggt atccaaaatt gtttttcctg caatgtccat atactgcttt
    11401 ggaatttcag accccatcct tttgcctttt ccccctgcaa caattattgc agtaacatat
    11461 ttttcattta gcatatcatc ccatccccct tccaattatg cggattaaag tgtgttccta
    11521 atccaacaat ttcttaatat tcaaatcatc cagtgacaaa ttagggactc ccaataacct
    11581 tgagggaata aaatcgccat gacagtccga ccctccggat atcatcaaat tattcctttt
    11641 gcagaaatca acataatatt gcgccttgtt tccgttatag ggggaaaagc actccattcc
    11701 gtcaattccg gcctccagcc agtactctaa ttcttccggg ggcatagctg tatccctgta
    11761 gtgataggat ggatgggaaa gaaatatttt ccctccgctt ttcttagctg tgcggattac
    11821 ttcctccggg cttttaagca ttgctttgag tcctgaattg taaacatcct ttaaatgctc
    11881 aggaatatct ttgtgtattc ctttatcaat catgtagttg gctgatttcc agcctcccct
    11941 tttcctgtca tattcatatc tttcataatc ctcggcactg acattatcgt atttgtttgc
    12001 agcataattg atataatcaa aattcgagct tagtttgttt tgatttgtcc agttcaataa
    12061 ctcaaggagc cttaagtcgt ttatatcgta attatataaa tttaaatgat annnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nataactcaa ggagccttaa gtcgtttata
    12241 tcgtaattat ataaatttaa atgatactct cttccttcat attcagcgga taattcagtt
    12301 cccggaataa aaattaaatt atccctgttc cttaaaatat cctgcatgtt tttaatattg
    12361 tcaacactgt catggtctgt aacagaaaat atttcaattt tatttttaat cagttcattt
    12421 tttaactctg tcgcgcccca tgtgccgtca gaggccatag tatgcatatg catgtcaacc
    12481 ttcttcataa ttgccttgta tgatagaaag aaatctatca taccttcctt tcttagtata
    12541 gtgaatcatc gtttatccgg gtataaaaca tggcttttac ttcttcaaaa tttttggttt
    12601 gagccataat ccacattaat tttgtgatta ccgcctcaat ggtcatgtca taagcctgga
    12661 gaacattata cttttgaagt gcctttactc ctacctcata tatgcccatg tcgcttccct
    12721 caaacattac ctgagttgca acaacaacaa ttttaccttt ttcaataagt ccttcaagct
    12781 tatttaaaaa attgtgtttg tcgagaaaag gaagtccgcc tgcaccgtag ctttcaatta
    12841 ccacgccccg gtattttttc cctatataat caagcacgtc ggcttccatg ccgggagcta
    12901 attttaacaa aaatatattt ggataaatgt ccttgtaaaa ttttacctct ttttcaagat
    12961 tgtaattgct gatatatttt gttatgcgcc tgtcatctat tatagccgcc accggaaaat
    13021 taatactttc aaaagcactg tagctttttg atttcacctt tctggcccgt gttcctatta
    13081 ttgctttacc gtcaaacacc acataaacgc cgcttacacc atcttctgaa gcgaacctga
    13141 agctgtccag aagatttttc tttgcatctg ttatttcaac atttattggt ttctgtgccc
    13201 ctgttacaac tattggtttg tttggatttt ggattaaata agaaagggct gcagatgtgt
    13261 atgccagtgt gtcagttccg tgagatatta caaaaccgtc atatttatga tagttttcct
    13321 ctatagcctc agtcatcatg acccagtgct ccggctgaat attggtgctg tcaatattta
    13381 taagctgaat tgtatctaca tggcaatact gccttatttc aggaacaaaa ctgataagct
    13441 cctctgaacc tatttccggt gccagaccgt tttttgtatt tattgaagca attgttcctc
    13501 ctgttgctat cattaaaagc tttttcatgc cttcctcctc ctttaacgga tattttccac
    13561 ttatcccgca gttccgcata caaatttatt tgttggtaaa ctcatcatcg ccgttgatta
    13621 tcttttccat atcctccggc aaagggcatg ttattatcat ttctttgttt gttaccgggt
    13681 gcttaaacct catttcataa cagtgaagcg cctgcctgcc tataagtgag ctttctgatc
    13741 cgtataaggt gtcgcctata accggatggc ctatatactt taagtgaact cttatttggt
    13801 gtgttctgcc ggttacaagc ttaagcttaa ccaatatcat attgttgaat attttctctt
    13861 ttttgaattc agttatgcat tccttgcctg atggatggac tatccgtatt atatcctcag
    13921 gattgagtct tgttatgggg gcatctattc ttccccggtc ttccttcata tggccttcaa
    13981 ctatggcata gtaatatttt tcaacctggt ttgacttcat ctggactgcc atctgattgt
    14041 gggcgtatgg attcttagcg attataacta tgccggatgt attcatatca agcctgttga
    14101 caaatcttac cttgcggtgg atgttgtttt ctttaaaata ataggcaagg ccgtttgcca
    14161 gtgtattgtt ttgatgggat cttgcaggat gaacaacaac gtagggctgt ttgtcaacag
    14221 caagcaaatc atcatcctca taaataattt ttgcggggat atccactgca gcgtattggt
    14281 cttcttcctc gtcaaattct atggacagtt catcgccttc aaagatgctt ttacttaatt
    14341 ttactgcttt tccatttagt aaaattttcc cggtccgctc taattttctt gagagtcttc
    14401 cggaaaattt aaatttttcg gtcaatatag tctttacagt cttatcattt tcttgtattc
    14461 tataattaag tatgttatta aacatttatt cactcctgtt gggttcaaac aatttctttc
    14521 ttccttgcgg ctgaagaaat tgtgttcaca cgaagaaact tggttggaga aattggaccg
    14581 tgaaattggg ttcgcaggta aattcactaa tttatttgtt ccttgcgtcg cataaattag
    14641 gaattttatg cgtttgacct accagccatt tttcagagaa aacactctgg aaattgggtt
    14701 agggcatctt gtgtacttat aataatataa aagattggta aagtaaaggg gataaaatga
    14761 aggagaaagt aataaatatt gctgctaacg atgactctta tacatctgaa attaaaagaa
    14821 aactcattag agttttaact gagaacggct ataattatta cgaaggtttc aacgaagatg
    14881 cacaattgaa tatcacagtg ggtggggacg gtgctttttt aagaggtgta agggcaagta
    14941 atttttcaca aattcccttc ataggggtaa ataccgggac cttgggcttt tatccggaaa
    15001 taacgcctga aaaccttgaa aactttataa ttaattacag ggcaggaaat tataccataa
    15061 attctgtaaa tttaatcgaa tgtgaaatag tgactgcaaa cttgtcaaac aagatttatg
    15121 ctgtaaatga tatcatatta aagagaaaag atatgaaaac aattcatctc aatgtattta
    15181 tcgcatcgca tcaccttgaa aaaataagcg gtgacggttt gatcatatct tctcccctgg
    15241 gaagctcggc atacaataaa tcagcaggcg gaagtctggt gtatccctca ttaaaaacac
    15301 tgcagataac accattgtcg ccaatattgt caaatgcata cagatgtttg gaatgcagca
    15361 tcatagtacc tcctgaattt gaaattgcaa tttatcctga aagaagagat gattactgcc
    15421 tggccatttt agcagacggg gaagttttcg aatacgatga aatggaacat gtgcttttta
    15481 gaacgtcaaa gaggataata aacagattga caaccggaac ctttaactac tggaacgtaa
    15541 taaaagaaaa gttcctgtag aggtatgggg acacaccgaa ataggtatag ggacacacct
    15601 taatgaaggg atagtctctg gggtaagcat taaggaatgt ccctaaattt ttatatttta
    15661 agagtgaact tactccctgc atttactgtg ctttctgccg ttagtgaata cttgtgcttg
    15721 tcggcaatcc atttggcaat gcttagtcca agtcctgtcc cgccttccct gtgccttgcc
    15781 ttgtccgccc tgtaaaatct gtcaaatatt ctatccagat ctttttcgct gatgcctatt
    15841 cctgtatctt caacaattaa aaacttttta tttttatcag tttttaagct tactttaacc
    15901 ctgcctttat ctgtgtactt aactgcatta tcaattaaaa taacggctaa cctacgcatc
    15961 ttgtcataat ctgccttgat tataatatta tcttcaatat ccgtaataag ttcaatgttc
    16021 ttttttgaag caataacttc cattgtctga cacacatttt ccactacttc agaaaaagaa
    16081 aattcagata tatccagttt ttcttcattg ttatcagcct ttgcaagaat taacagttga
    16141 tcaatcagct tagccattac tcttgtttca gaatatgcat tgtcaagcca gatttcattt
    16201 tcttcgactg ttccatcttc atcgcttaaa accgcatcta aatttgtttg tattactgta
    16261 agaggagttc taagttcatg ggacgcatct gccacaaact ctctctgcct tacccatgtt
    16321 tccctgacag gtgccagtgc cttgcctgtg agaatatagc ttaatgccag caaaacaacg
    16381 gtgccgcaaa ttcctataac agtcagaacg tttcttaaga atgtccacag tatttcttca
    16441 tttactgtat tttgataaac ctgaactatg ctatggccgg taatatcatt aaaatatctg
    16501 gtataaattc tgtagttgaa tctgcctatc ctttttgtcg tataagaatc ttctttttct
    16561 tcaattgcct cctgcgccag gggctttata taatcagcta tttccgtatc accttcatcg
    16621 gataaaacag gcgttaaatt ctggttataa acaatataac aggcatccag ccttttagta
    16681 tctgagggat tgtttatata ccttctgaat aaccctccgt taaagaagta atccgcatag
    16741 gctgtatttt tatagttgta aattctcagt gcttctgccc tcattagctc tttgttgccg
    16801 tcttcaaata taatttttgt aaatttgtat attcccgcag aaacaaaaaa cataaaaaga
    16861 ataagtatga tacccatgta tgcggtcagt ttcaaatgaa gtttcctaaa catttatttc
    16921 tccttaagca tatagcccac gcctcttacg gtttctattt taacatttgt atcctcaagt
    16981 ctttttctta aatggtgaac atatatttca atattgcttt caagtatatc cgcttcaagt
    17041 ccccatatgc ggtcgataat ctgttctctt gtaaaaacct gacggggttt tcttatgaac
    17101 atttccataa gcatggcttc tttaagaggt attttatatt cagtatctcc cacatacaag
    17161 agatagtttt tgacgtcgta tttaatatct tcaaattcaa atacttcacc tgcgaattcc
    17221 gtgctttttc tccgggctaa agctttgacc ctggcaatca attcctttgt ggcaaaaggt
    17281 ttaaccaggt aatcatcagc ccctgtttca aggccgaaaa cccggtcatc caccgtatcc
    17341 tttgcagtaa gcatgattac aggtgttttt acgccttcct tcctgattgt tttcagtatt
    17401 tgaatgccgt caattcccgg cagcataata tctaaaacta tcacgtcata aatatccttg
    17461 ccggcaagaa tcagtccttc ttcaccgtca tttgcaatgt caacgtcaat tttttgctta
    17521 ttaaacagaa aattcagcgc ttgtgtaatt tttacctcat cttcaactat tagtatcctc
    17581 aagtgcaata cccccattat acattgtaat ttactatttc acaggtccct tcattctgaa
    17641 gactgttaat ttttccatgt atgattatac tataccaaaa accttaaaat tatattaata
    17701 ttaagtacat aatataaact gaagtcaggt tttgtcaagc acttacaata tgccggccaa
    17761 taaaaaagaa gcctttcata ttaatttatg aaagacttca tatatcagca atatattagc
    17821 atttagaata tttagcctct aaatctatct aaactgctat agaaagaatt cggggattac
    17881 atcattccca ttccgccgcc gcccattgga ggcattgccg gttcttcctt aggctcatct
    17941 gctacagctg cttccgttgt caatagcatt gcagctacgc ttgctgcgtt ttgaagggct
    18001 gaccttacta cctttgtagg gtctactata cctgattcaa ccatatttac atatttttca
    18061 gtcattgcgt caaaaccgac acccttattg ctgtgtttga ttttttcaac gactactgag
    18121 ccttcaagac ctgcattgat tacaatttgt ctaagtggtt cttccaatgc cttttcaata
    18181 atcttagctc ctgtttttgc gtctccttcc agtccttctg ctactccggc aactgcacct
    18241 attgtattta aaagtgcaac tcctccgcct ggtaccagac cttcctcaac ggctgctctt
    18301 gtagcattta aagcatcttc aattcttaat ttcttttctt tcatttcggt ttctgtagct
    18361 gctccaacat tgataactgc tactccgcct gtcaatttag ccagtctttc catgagcttt
    18421 tctttgtcga attcagatgt tgattcttca tgctgtgctc ttatctgttt agctctttca
    18481 tctatgtctt ctttagcgcc tgcgccgttt actattactg tattttcctt gtctatttta
    18541 actgttcctg cattacccag catttctaag gtagtttctt ttagatcaag tcctaactct
    18601 tctgatatta cctgaccgcc tgtaagaatg gcgatgtctc ttaacatttc ttttcttctg
    18661 tctccgaatc ccggcgcctt tactgcaaca cagttaaatg ttcctctcaa tttattaacc
    18721 accagtgttg ccagtgcttc accttctaca tcttctgcta taatcaggag tgatttgcct
    18781 gtttgaacta tttgctccag aatcggaagt acttcctgaa tattggttat tttcttgtct
    18841 gttattaaaa tatacggatt atctaaaaca gcttccattt tttccgtatt agttaccatg
    18901 tatgctgata agtatcctct gtcaaactgc attccttcaa caacagtcag ctctgttccc
    18961 attgtcttgg attcttcaac agttataaca ccgtctttgc ccacatcatc cattgcctgt
    19021 gatattaatt gtcctatttc ttcatctcct gcggatatgg atgctatttg tgctatttct
    19081 tctgatgttt caactttctt gcttatactt ttaagctcat ccacagcttt tgaaactgct
    19141 ttttcaatac cctttttaag tatcatgggg tttgcgcctg ctgcaacatt tttaagtcct
    19201 tctcttataa ttgcctgagc cagcagagtt gctgtagtag ttccgtcacc ggctacgtca
    19261 tttgtctttg ttgcaacttc ttttaccaat tgtgccccca tgttttcata agggtcggac
    19321 aattcgattt ctcttgctat tgtaactccg tcattggtta tttgcggcga accgaaagat
    19381 ttgtgcaaca ctacattcct tcccttaggt cctaaagtaa cctttacagt atctgccaaa
    19441 gcattaacgc ctctttccat tgagcggcgt gcatcttcat caaatttaat tatttttgcc
    19501 ataatatctc tccttttttt attcaataac tgctaataca tcagctaatt taattattat
    19561 tacttcttca ccgtcaattt taacttcggt tcctgaaaat ttagagaaaa taaccttatc
    19621 tccaaccttg atttcatcct ttttcttttc atcgtttgta atccctgcac caactgctag
    19681 aacttcagca acctggggtt tttcctttgc ggagcccggc agcactatgc cgcttttggt
    19741 tttttcttcg gcttcagtca tttttataac tactctttcg cctaatggtt ttattgtcat
    19801 attaattcct cctattataa atgttattat ctgcgatttt tgttagcact cgtcctcgaa
    19861 gagtgctaac acctagtctt tatattactc atttatctta atgttttcaa cccctgttta
    19921 ttttgatttt tgcctgaata tatatttatt attcactttt tccttcattt cctttatttt
    19981 gctttaagtt gctctgtttt tcatttataa aaaatattag tgctactgtc atttgtgaca
    20041 gcacccctgt ccggtgtcgc gtccctttcc tttgaacctt aattaaatat ttttaaataa
    20101 gagatgtttt ttatattgat atgtagtata attaatgaat agtacaatgc ataggaggaa
    20161 aaagtatggc tcttgtaaca tctcaggaaa tgtttaagaa agcatatgaa ggcaattatg
    20221 ctgtaggcgc cttcaatgta aacaacatgg aaattattca aggaatagtg gatgcagcaa
    20281 aacaggaaaa tgcgccgctt atacttcaag tttcagcggg tgcgagaaaa tatgcaaatc
    20341 ctatttattt aagaaagctt gtggaagcag caattgaaga cacaggacta gatatctgtc
    20401 ttcacctgga ccacggtgaa gattttgaaa tttgcaaaca gtgtgttgat gatggttttt
    20461 catctgttat gattgacggc tctaaatatc ctttagaaga aaatatagcc ctgactaaaa
    20521 gagttgtgga gtatgcccac tcaaagggag ttgttgttga aggggaactt ggcaaactgg
    20581 caggagtaga agatgcagtt aaagtaaaaa ctaaggatgc tacatatacg gacccgaatg
    20641 aagctgtgga attcgtggaa agaacaggag tcgactcgct ggcgattgca atcggaacga
    20701 gccatggcgc ttataaattc aagggagatc caagcctgga ttttgaaaga ttagaaatta
    20761 taacaaacct tctgcctggt tttccgttag tattgcacgg ttcatcctct gtgcccaagg
    20821 attttgtgga tttatgcaac aaatacggag gacaaatccc gggagctgca ggagtgcctg
    20881 aggaaatgct gagacgggct gctcaaatgg gggtatgcaa aataaatata gatacagact
    20941 tgagactggc tcttactgca tgtatcaggc aatacttctt tgaacatcct tctgaattcg
    21001 acccgagaaa atacttagga cccggaagaa cggcaattaa agatatggtt gctcataaaa
    21061 tgagaaatgt tttaggatgc agcaataaaa gataaactaa ggctgagctt ctgctcagcc
    21121 ttacactatt gataaactat atttatgaga ttcttcaggc tgcgccttca gaatgacatc
    21181 ataggaatat gctctattat tttaaatcgt tcatatatgt tgaaacataa tttttgtcaa
    21241 ctgttacaac
//
LOCUS       DAOPUT010000017        20999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_2521_length_20999_cov_18.904746, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000017 DAOPUT010000000
VERSION     DAOPUT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 20999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20999
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2521_length_20999_cov_18.904746"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..443)
                     /locus_tag="PKK75_03075"
     CDS             complement(273..443)
                     /locus_tag="PKK75_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82607.1"
                     /translation="MRKFILMILLILVMLSFDGCDTRKDVSETPNTMSPSIMADGELY
                     STTEGEMPIEPY"
     gene            821..1963
                     /locus_tag="PKK75_03080"
     CDS             821..1963
                     /locus_tag="PKK75_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82608.1"
                     /translation="METLLEKLSQFEFLYSVKTNPYLPIVNFIVSRGFGADAASTEEV
                     MIAHGLGLSHEKILFSSPGKTRKDIEKTLDKSIIVADSYNELALINDVAKQRGLHIKA
                     GLRINPDYSMDFGKGASSKFGVDEETIVCQRDFLNNLTNISIVGIHVHLRSQVLEHSK
                     LYRYYEKIFELALFCKESMGWEMEFINFGGGLGIVYSSLNDIPLNLELLSVEFEKLLL
                     RFKDKISARLIIETGRFVVCEAGKYVTRIVDIKESRGTKYLVVENGLNGFLRPSIAEL
                     LTAYTPQDIKLQGCEPLFTSKDAFEFAILGKEKLPAEKVSIVGNLCTSADIMAKDIIL
                     PKADIGDILVVSKAGSYSYTLTPTLFSSHPLPLQFYLNENCEKCIL"
     gene            2021..2701
                     /gene="hypB"
                     /locus_tag="PKK75_03085"
     CDS             2021..2701
                     /gene="hypB"
                     /locus_tag="PKK75_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNZ82609.1"
                     /translation="MTDIKLIKIKESITADNDRSADLIRNELANNKVFMINIMSSPGA
                     GKTSLIIATMKILQNKYHINVIEGDIDSTVDSEKIIRNGGDAVQIQTGGDCHVDASMI
                     KAAIETIDLKDYDVIFIENVGNLVCPAEFDTGANLKVMILSVPEGDDKILKYPLMFNV
                     CDALIINKIDCIEFFDFDFNVLKNRVSLINPKMKIFQVSAKTGEGIIEWCDWLSKQID
                     CYNKSLNL"
     gene            2705..3064
                     /locus_tag="PKK75_03090"
     CDS             2705..3064
                     /locus_tag="PKK75_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNZ82610.1"
                     /translation="MCPIHELPLTQKILSVVLQYAEFNDAEKVLEVCIEIGELRDFVE
                     SITQKYWDYLSKGTIAEGAKIKFYRVPVSSICHSCSNIFSFDFRKPEKSSCPVCGSFD
                     SELLTGTELEVKEILIL"
     gene            3140..4279
                     /locus_tag="PKK75_03095"
     CDS             3140..4279
                     /locus_tag="PKK75_03095"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ82611.1"
                     /translation="MENDLYKKLAKHLDNLPGGFPETDSGVEIRILKRLFTPEDAELA
                     LTLTLIEEEPHVIAKRAGISAEEATKRLSEMEKKGLIYGTHSKDNIDRYMALHFIIGI
                     WEFQVDKLNPDLVRDVDEYLPYWADVDTWKNVPQIRSIPVGEAIDSQLEITSYENAEE
                     LVDKQDKIAVAPCICRTEQQMIGKGCDKPLETCLSFGSGAEFYVRNGMGRFITKDEAF
                     NILKQANTAGLVLSPGNSQKATFICACCGCCCGVLRTLKTYPKPAEIVSSPFVARHNK
                     DTCIDCSVCVERCQMDALQSNNGKITLDKERCIGCGLCVTTCPTDSLILVRKPEDWQH
                     AVPKNFTLMNIKLGQHRGKLSMGRLMKMLVKSKLDRINSPKKWNL"
     gene            complement(4403..4891)
                     /locus_tag="PKK75_03100"
     CDS             complement(4403..4891)
                     /locus_tag="PKK75_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82612.1"
                     /translation="MNPMISKFVDSKFMTEEQAQYVENAINNNESIVVSGHRSTGTRP
                     FMATLMGVAKKQYSTVQVKGFDDINADTEYLLILALDNLDFEALVGDAIGAKAPFISI
                     KEPEHPVSLMKVMKQQFKLGKGIGKKIHTIEAKKVDGVPYVDKIIEFCMDESGKIVKT
                     DL"
     gene            complement(5173..5448)
                     /gene="spoIIID"
                     /locus_tag="PKK75_03105"
     CDS             complement(5173..5448)
                     /gene="spoIIID"
                     /locus_tag="PKK75_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HNZ82613.1"
                     /translation="MKDYIERRAMEIAEYIISTESTVRQTAKKFGVSKSTVHKDVTER
                     LPKLNPPAASEVKKVLLKNKSERHIRGGKATRLKYKEAHEHEKVSNM"
     gene            complement(5525..6253)
                     /locus_tag="PKK75_03110"
     CDS             complement(5525..6253)
                     /locus_tag="PKK75_03110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ82614.1"
                     /translation="MKNNRLARAKTKMKEFFIKLKHKDTSFLVIALVILLLATAVVWR
                     YTSRPGDGGTDLAEGQTPNGDIEVHEDPYEDYVQDIIDNFQQAENDDDDEDEDDGVKT
                     QQMDLKSMNAPVGGEIIRKFTIDDLVYYEAIGEWRVHKGIDIKPKDTLLIESALAGKV
                     EAVNNSEITATEIIIDHGNNVKTLYSNLVSANVKAGDTVEKGQVIGNLGKTVSIEASD
                     GAHLHFELIVDGKQINPLDYITIE"
     gene            6390..7424
                     /gene="spoIID"
                     /locus_tag="PKK75_03115"
     CDS             6390..7424
                     /gene="spoIID"
                     /locus_tag="PKK75_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HNZ82615.1"
                     /translation="MLNRLIYAFVVFAMLLAIPNLSLNFFEKKMNLTSAAVHEGDSSK
                     YADDKNQEKYTVDVYEPELIKVYNNKTNDVMVIDFEEYLKGVVASEMPAEFNVEALKA
                     QAVSARTYLLYRLKKYPDGQPEHLGAPVCTSIHCQVWSSKDELIQSHYDGWYDEYMDK
                     IEVAVESTRGQILTYEGKIIEPLYHSTSGGRTENSEDVFSAAVPYLRSVESPYEDESP
                     RLNSSVKITTGEFIDKIESAYGDMDITESNLDEKIKLGEVSEGGRIKTLIIDNTEISG
                     RDMRALFNLNSTNFSFIQSGSQIEILTTGYGHGVGMSQWGAEGMANKGYNYKEILKHY
                     YTGVEIVNMR"
     gene            7551..7874
                     /locus_tag="PKK75_03120"
     CDS             7551..7874
                     /locus_tag="PKK75_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB-like protein"
                     /protein_id="HNZ82616.1"
                     /translation="MIFIDDTAYKEFKELLDNANVDSYNVRISLDRVGCSGPIFGVFI
                     DEANEDDEVEVINEITFISEKSIVEEYGGFIIVSSEENMGRGVGMKPVVAPSPSCDVC
                     GGGCH"
     gene            complement(7949..8221)
                     /gene="rpsR"
                     /locus_tag="PKK75_03125"
     CDS             complement(7949..8221)
                     /gene="rpsR"
                     /locus_tag="PKK75_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNZ82617.1"
                     /translation="MSSRQGGKDMGDKNEKYSQRRKPRKKVCQFCQEKATHIDYKDLG
                     RLKKYTTERGKILPRRITGACAKHQRMVTRAVKRARSIALLPYVAE"
     gene            complement(8248..8706)
                     /gene="ssb"
                     /locus_tag="PKK75_03130"
     CDS             complement(8248..8706)
                     /gene="ssb"
                     /locus_tag="PKK75_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ82618.1"
                     /translation="MNNVVLIGRLTKDPDLRFIPSTGMAVAKFTLAVDRGIYGEKKQE
                     AINQGKPTADFINITVFGKSAEVCANYLSKGRQCAIQGRIQTGSYTTQTGEKRYTTDV
                     IADKVEFVGAKDQSSAPQGNAVQPDSSFFNDFPDEDNDIFEPVDDEDIPF"
     gene            complement(8729..9016)
                     /gene="rpsF"
                     /locus_tag="PKK75_03135"
     CDS             complement(8729..9016)
                     /gene="rpsF"
                     /locus_tag="PKK75_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNZ82619.1"
                     /translation="MRKYEGAFILLSNLEEDVRNAEIEKVKSIITERQGTVEKVNEWG
                     HRRLAYEIDKKREGYYVFIEFTAGADAVDEIDRICKISDNYIRHMITVEEE"
     gene            complement(9106..9267)
                     /locus_tag="PKK75_03140"
     CDS             complement(9106..9267)
                     /locus_tag="PKK75_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxH/CxxC protein"
                     /protein_id="HNZ82620.1"
                     /translation="MKEKYEEIFACKDHADQALDDYVNKNEAAPELIKTSEEKKCSYC
                     QKNAEYIIK"
     gene            complement(9275..10063)
                     /locus_tag="PKK75_03145"
     CDS             complement(9275..10063)
                     /locus_tag="PKK75_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ82621.1"
                     /translation="MKLKFCSLYSGSSGNCQYIKTDKATIIIDAGLSGKRIQQEIVNI
                     GEDPKKIDAIFITHEHIDHIQGAGIMSRRLNIPIYANEKTWEAMSPLLGDIEPHNIKI
                     LDDCTQIGDLTIKPFDISHDAAHPVGYNIFYEHKKLSLVTDTGCFNGTITNSIMDSDL
                     LLVESNHDEDLVIIGPYPWPLKRRVLGEFGHMSNETAGRLIANVVKRGTEIVLLGHLS
                     KENNFPELAYKTVESILLEESIEVNPGICLDLTYRDRSSKVYEL"
     assembly_gap    10184..10283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10293..11351
                     /locus_tag="PKK75_03150"
     CDS             10293..11351
                     /locus_tag="PKK75_03150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ82622.1"
                     /translation="MIIKFKWKSLLIFIAIMLIVSAVYLFINRAQSVSADEEKSFIKW
                     VDFRVSYDAMDRALRADINSIDEEVKLDWIEILAYLGTRYGGDFFSRYKAKDMDNLIN
                     RLKSGESIESMTKDLAHYNYYYEAYKAVLDGFVGPYEIQVKDENTGEKVWVKKYGLKA
                     FSPIGGGYHYGHYDDFGNSRNYGFRRVHLGNDLVGSIGTPVIAVESGRIEALGWNQYG
                     GWRMGIRSLDNKRYYYYAHLRKDHPYVKTLKEGDIVLAGDVIGYLGMTGYSRKENVNN
                     IRTPHLHFGMQLVFDESQKEALAEIWIDVYRIVKLLNRNRSPVAYNKETREFDRIYDI
                     RFHNIERGKWKGDTHNEL"
     gene            complement(11513..12931)
                     /locus_tag="PKK75_03155"
     CDS             complement(11513..12931)
                     /locus_tag="PKK75_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ82623.1"
                     /translation="MKTINRRIILIIILLSIFNTTVYAGVNPPRKEIEAMIDEVALKR
                     GVPAILLKGIARVESVYKHFNSDGSPKICGTSIGLMQINNIYGGYDNYRLKYDILYNI
                     EAGADVLLNKWSMSSYKQVSSVGNMDPDVLENWYFALWAYNGWAQSNNPVSPYAKKYT
                     YQQLVYDVIEKEYGKKISNIDFSYLPRDSKPSRSLTVPTPVSCSSGGIILYEKDDYVR
                     TDGIRGAYYLRDAPAGNYVHQLSVNQLGTITEGPVLKNGYYWYKVYIDETKEGWIERN
                     WLVRTGDTQYGRYIYEDISFHWARKIIMDMYGKEIVSKAALYNPDTPVTMEEFCIFLS
                     RTLNYIENANQQSRIRKENNLLFYDYTNVHPWALNYVKDIYERGLLEAVHPLEMLNRK
                     TAVLLIEDILVSSHKFDEIDINLIFSDLKNLTEEEIQAVKTAYANGVVSGKHIKAFCP
                     DECLTRAEAAVMMANIVNIYVR"
     gene            complement(13139..14674)
                     /locus_tag="PKK75_03160"
     CDS             complement(13139..14674)
                     /locus_tag="PKK75_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ82624.1"
                     /translation="MKIFKSTARKVILIIFTFVLALNTFAYAGVNPSREELEFMIERV
                     AEKRAIPSILLKAIARVESCYEHYNQDGSPKITGTSIGLMQINNRNGGYDTDKLKYDI
                     MYNIEAGADVLLSKWSMSSYNIVPSVGNMDPNVLENWYFALWAYNGWASSNNPNTISS
                     YAKKYTYQQLIYDVIEKEYDGKVHNIDFSLLPSTGKPSRSLVVPTPMYTNGGDIIFYD
                     KGDYVRTDGMREKFYLRDGPAGRYIHDISLNQLAVIDEGPVLQNGYYWYKVYIDENTA
                     GWIERNFLLRTGDIEYGRYVFEDISFHWARKIIMELYGKDIVSQAQYFNPDNFISKEE
                     FLILLSKTLDYAQYENAGSEELMSTKDEEPSNEDYKSSMENDKSLESEIVTQGAAPVL
                     TTVVESINPWALEYIADVYNLGLVEEEDLLAPLESLSRKEAALIINCLFEIKEEFSSL
                     DMETIFTDINSLSEEEIAAVKTVYTNSIMTGKTSSGFCPDSYLTRAEAAVIMDKVSEK
                     LMP"
     gene            complement(14705..16417)
                     /locus_tag="PKK75_03165"
     CDS             complement(14705..16417)
                     /locus_tag="PKK75_03165"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNZ82625.1"
                     /translation="MHNEKFKIWLNSPYVDDELKKELKEIEKLDNEIEDRFYRELEFG
                     TGGMRGKIGAGTNRMNLVTVAKATQGIADYLIEKHKNEEISAAIAYDSRNMSREFAEK
                     TACVFAANNIKVYLFDDLRPTPELSYAVRHFNCKAGVVVTASHNPPEYNGYKVYDEFG
                     GQVVQDADTIIEKINKVKLADIKLSVFKGNAMIQMAGNDVDTSYINEIKSLSLRDDID
                     KDIKIIYTPLHGTGNKPVRRVLDEMGYRNVFVVEDQEKPDSQFSTVKSPNPEDISSFE
                     LAIKKAGEIDGHIIIGTDPDCDRVGAVVKHKGEYVPLNGNQTGALLLNYLLESLNEQG
                     RTPESPAIVKTVVTSEVANCIAGKYNVTVFDTLTGFKYIAELIQKFKETKEHNFLFGY
                     EESYGYLAGTFVRDKDAVIASMLICEMAAYYKKLGKTLIDVLDEIYEEHGYYIEETIS
                     LSFSGLAGQSKMKAIMEYFRENKLYSLAGKKIPAINDYKMSISLDLIKGTTVKLNYPK
                     SDVLKFCFEDGSWLVLRPSGTEPKLKVYFSINGKSREKSKEALEKAKSEILNIIDRIQ
                     INIG"
     gene            complement(16429..17295)
                     /gene="galU"
                     /locus_tag="PKK75_03170"
     CDS             complement(16429..17295)
                     /gene="galU"
                     /locus_tag="PKK75_03170"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNZ82626.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKAIPKEMLPIVDKPTIQYIVEEI
                     AASGIEDVLIITARNKSSVEEHFDRAVELEYILKDANKEELLEEIKGISRMINIHTVR
                     QKDPLGLGHAIYCARAFVGDEPFAVLLGDDVVDSEKPCLKQMLEVFEEYKSTILGVQP
                     VEWDQVHKYGIVSGERINDRIYTVNDLVEKPHKESAPTNIAILGRYIITPKIFHILEN
                     TKPGIGGEIQLTDGLRELCTTEEMYAYIFQGRRYDVGSKIGFLEATVDYALKSDELRE
                     DFKKYLKSINLE"
     gene            complement(17373..18404)
                     /gene="mreB"
                     /locus_tag="PKK75_03175"
     CDS             complement(17373..18404)
                     /gene="mreB"
                     /locus_tag="PKK75_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HNZ82627.1"
                     /translation="MSLRMDIGIDLGTASVLVYIKGKGIVINEPSVVAIDINTNKILE
                     VGEEARKMLGRTPGNIVAIRPLRDGVISDFDITEKMLKYFIKKAVGNSLVKPRVIICV
                     PSGVTEVEKRAVLEASNSAGAKKTYLIEEPVAAAIGAGLDITKPSGHMVIDMGGGTTD
                     IAVISLGGIVVSRSIKVAGDKCDDAIVRHVRKKYNVMIGLRSAEELKIKIGTAFPIEE
                     ERLMEVRGRNLVTGLPVNLTISSSDSLEAMEETITTIADAVHSVLEKTPPELAADISE
                     KGIVMTGGGCLIHGMDKLLEKRTGLKVTIAEDAVSCVAKGTGQSLEHMDVLKDALISE
                     HIKTNYNVG"
     gene            complement(18500..19654)
                     /locus_tag="PKK75_03180"
     CDS             complement(18500..19654)
                     /locus_tag="PKK75_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNZ82628.1"
                     /translation="MNINDFTFEEPSPFGRLSIIAMKGCEDIASRIDYYLKDWRQDAN
                     GVIYSNGSGPIGTFLIKAACPRFGTGEAKGVLYETVRGHDIFIISDVFNYGVNFNMYG
                     TEVPMSPDDHYQDLKRIIAALNGKAKRITVIMPMLYEGRQHKRAARESLDCALALQEL
                     VNMGVENIISFDIHDPRVQNAIPLHGFEDFHPYYQMIKAFVRTVPEAIIDRDHMMIIS
                     PDEGAMTRCIYYSSVMELDLGMFYKRRDYTVVVNGKNPVLSHEFLGGNLEGKDVIIVD
                     DMIASGDSLIDVAGKLKERSARNVYAFATFGLFTEGISKFDEAYRDGILSKVFTTNMI
                     YRKPELFDKPWYAEVDMSKYIANIIDTLNYDNSLSELLDPVQKIKKLLKK"
     gene            complement(19820..>20999)
                     /locus_tag="PKK75_03185"
     CDS             complement(19820..>20999)
                     /locus_tag="PKK75_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ82629.1"
                     /translation="DMKYNASNEEVGREELKIIAERQNEKMAALGQLAGGVAHDYNNQ
                     LMSIIGNATMIQKTDDLNRIYDYAERIIHISQSAAELTKKILMFSKKKSSTNKPVNLK
                     NIMDNTYKMMEFILSKEIDLIYSYEANDEVILGDECQLESMIVNMILNSKDAIKDYGM
                     IKIGTTDTVILSEMALSHGEIIQPGQYIKIYIEDNGSGIDEDILLKIFEPYFSTKNKS
                     NGTGLGLSVVFGTVKSHTGYLNVTSSPGHGTRFEIFIPALEKTEAYEKKKIEDKDNNI
                     VMLVDDDINVLDIEAEMLKDLGYDVVKFNSPLESLKYYEAEYKKISFCVLDIMMPELN
                     GRELYEKILLINEEAAAIFITGFVQQAEYEELMRRGLTIIEKPFTYEVLSETIAKMYF
                     "
BASE COUNT         6001 a         4050 c         3689 g         7159 t
ORIGIN      
        1 agaaatagaa aaaatcaaaa tacaaaatat tttttagatt atagagttaa aaaatgatat
       61 ttccataacc ttctacactg gctatgattt gagttttata taactgtact ccaattccgg
      121 tcataaatgt ggtaaacgaa ccggccagct taatattctt ctacgccatt aattttcgca
      181 tactttgcat tcggaactgg aaaattgatt tccccctctt ttgctggtaa ttcagtttcg
      241 ttaattattg aagtaacagt attaattgca ctctaatatg gctctattgg catctctccc
      301 tcggttgtag agtacaactc tccatctgcc attattgatg gagacattgt gtttggtgtt
      361 tcagaaacat cctttcgagt atcacatcca tcaaaggata gcatcactaa aattaacagt
      421 atcattaaaa taaattttct catcttactg ctccttatac tacagtttca tatatacgac
      481 gcatagtaat actttattcg gctcaatata agaaaaatgc gaattaatat aaccatatgc
      541 ccaaatgact tgatttttga tttttgcaaa ggtctgcttt ttcaatgtga tttaccttta
      601 aaatttgcat ttaatatgta aataagttac aattatagca tgtttcaatt atatattatt
      661 actattaaat atgacacact tttatctatg tctcatattt ttttaaaatt tgcaataaag
      721 aggatttatt tatggaaaat aatttagaca ttattaaaaa attgatggaa gaatatgaag
      781 acctttctac atttataatg aaggtgttat ttctaagcaa attgaaacac ttttagaaaa
      841 attatcacag ttcgagtttt tatattcggt aaaaactaat ccctatttac ctattgttaa
      901 ttttatagta tccaggggat ttggagcaga tgcagcttca actgaggaag taatgatagc
      961 acatggatta ggcttatctc atgaaaaaat attattttca tctccgggaa aaacacgtaa
     1021 ggatattgaa aaaactttag ataaatcgat aatagtagca gacagctata atgagttagc
     1081 tttgattaat gatgttgcaa aacagagagg attacatata aaggcaggat taaggataaa
     1141 tcctgattac tctatggatt ttggaaaagg tgcaagcagc aaatttggcg ttgatgagga
     1201 aacaatagtt tgtcagagag actttttaaa taatttgact aatataagta tcgttggtat
     1261 tcatgttcat ctgcgctctc aggttcttga acacagtaag ctttatcgat actatgagaa
     1321 aatctttgaa ttagctttat tttgtaaaga gagcatgggt tgggagatgg aatttattaa
     1381 tttcggagga gggcttggta ttgtatattc atcattaaat gatattccac ttaatcttga
     1441 attgcttagt gttgaatttg aaaaattgct cctaagattt aaggataaga ttagtgcaag
     1501 gctaatcatt gaaacaggca ggtttgtagt ctgtgaagcc ggtaaatatg ttactcgtat
     1561 tgtagatata aaggagtcca gaggaaccaa atacttagta gtagaaaacg gacttaatgg
     1621 ttttctaagg ccttctattg cagagctgtt aacagcatat actcctcaag atataaagtt
     1681 gcagggttgt gaaccattat ttacatctaa agatgccttt gaatttgcta ttcttggtaa
     1741 ggaaaagctc cctgctgaaa aagtaagcat agttggtaac ttatgtacgt cagctgatat
     1801 aatggcgaaa gatataatac ttccaaaggc tgatattgga gatatattag tagtgtcaaa
     1861 ggctggaagt tattcatata cattgacacc aacattattt tcaagtcatc ctcttccatt
     1921 acagttttat ttgaatgaaa actgtgaaaa gtgtatactc taagcagtaa atctattttg
     1981 ggtataaata aataagaaat aaaattattg ggaggcagca atgacagata taaagttgat
     2041 taaaattaaa gaaagcataa ctgcagataa tgaccgttct gcagatctaa tacgcaacga
     2101 actggcaaat aacaaggtat ttatgattaa tattatgtct tcgcccggtg caggtaaaac
     2161 aagtttaatt attgccacta tgaaaatact gcagaacaaa tatcatatca atgtaattga
     2221 aggtgacata gattcaacgg ttgactccga aaaaattatt cgaaatggag gcgatgcagt
     2281 acagatacag acaggtggtg actgtcatgt tgatgcttcg atgataaaag ctgctataga
     2341 aactattgat ttgaaagatt atgacgtaat ttttatcgag aatgtcggaa accttgtgtg
     2401 tccggcagaa tttgacacgg gtgctaacct gaaagtaatg attctcagcg tacctgaagg
     2461 ggatgataag attcttaaat atcctcttat gtttaatgta tgtgatgcat taattatcaa
     2521 taaaattgat tgcatagaat tttttgactt tgactttaat gttttaaaaa accgtgtttc
     2581 tttgattaat cctaaaatga aaatatttca ggtttctgcc aagacagggg aaggaataat
     2641 tgagtggtgt gactggttat caaaacaaat agactgttac aataaaagtc ttaatttata
     2701 ggaggtgtgt cctattcatg aattgccttt gacacaaaag attctatctg tcgtattaca
     2761 atatgcagaa tttaatgatg ctgaaaaagt tttagaggtt tgtattgaaa ttggagaatt
     2821 gagagacttt gttgaatcaa taacacaaaa gtattgggat tatctgagca aaggaacaat
     2881 agccgaagga gcaaaaatta agttttatcg agttccggtt tcatcaattt gtcacagctg
     2941 cagcaatatt tttagttttg attttcgaaa gccggagaaa tcaagttgtc ctgtatgcgg
     3001 aagctttgac tcggaacttt taacaggaac agaacttgaa gtaaaggaaa ttttaatttt
     3061 gtaatgaatc tgcaaataaa taattcggtt gttatgagaa aaatacaatt tagaacaatt
     3121 ttttcagtgg gtgaaaataa tggaaaatga tttatataaa aaattagcta agcatttaga
     3181 caatttaccg ggaggatttc cggaaactga tagcggggta gaaatacgaa tattaaaacg
     3241 cctttttaca cctgaagatg ctgaactagc tttaacactg acattaattg aagaggaacc
     3301 tcacgtgatt gcaaagcgtg ctggaatatc tgctgaggaa gcaactaaac gattatctga
     3361 aatggaaaaa aaggggctca tctacggcac gcattcaaaa gataacatcg acagatatat
     3421 ggctttacat tttattattg gcatatggga atttcaagtg gataaattaa atcctgattt
     3481 ggtacgtgat gttgatgaat acttgcctta ttgggcagat gttgatactt ggaaaaatgt
     3541 gcctcaaatc agatcaatac cggtgggaga agcaatagac tcacagttgg aaatcacatc
     3601 ctacgagaat gcggaagagt tagttgataa acaagataaa attgcagtgg caccgtgtat
     3661 atgcagaact gaacagcaaa tgatcggaaa aggctgtgac aagcctttag aaacatgtct
     3721 gtcattcgga tcaggagctg aattttatgt gcgaaatgga atgggacgtt ttattactaa
     3781 ggatgaagcg tttaatatcc taaagcaggc aaatactgcc ggacttgttt tatccccggg
     3841 aaactcacag aaagctacct ttatttgcgc ctgctgcggt tgttgttgcg gggtgctaag
     3901 aacattaaaa acttatccta aaccagctga aatcgtatct tcaccatttg tagcccgtca
     3961 taataaggat acctgcatag attgtagtgt ttgtgttgaa cgatgtcaaa tggatgcatt
     4021 gcagtcaaat aacggaaaaa taaccctgga taaagagcgc tgcataggat gtggtttatg
     4081 tgttacaacc tgcccaacag attcattaat tcttgttaga aaaccagaag attggcagca
     4141 tgctgttccg aagaatttta ccctgatgaa cattaagctg gggcagcatc gtggaaagct
     4201 ttcaatgggc agattgatga aaatgttggt aaagtctaaa ttagacagaa taaattcacc
     4261 aaagaaatgg aacttataaa actcctttat tcaatgttaa gattttctaa tcccaacaat
     4321 ttataaatgg ccggaataat aaaggggcat agttgtcagt tgacaccatg tccctttgtt
     4381 taatgtaaat attcttttta cattaaaggt ctgtcttaac tatttttccg ctttcatcca
     4441 tgcagaattc tataatttta tcaacgtatg gaacgccatc gactttctta gcttctatag
     4501 tatgaatctt tttgccaatg cctttaccca gcttgaattg ttgtttcata accttcatta
     4561 atgatactgg atgctcaggc tcttttattg agatgaatgg cgctttagca ccaattgcat
     4621 ccccaacaag tgcttcgaag tctaagttat caagtgctag aattaatagg tattctgtat
     4681 ctgcatttat atcatcaaag ccttttacct gaacagttga atattgtttt ttagctactc
     4741 ccattagtgt agccatgaag ggacgagtac ctgttgatct atggcccgat actacgattg
     4801 attcattatt gttgatagca ttttcaacat attgtgcctg ttcttctgtc atgaattttg
     4861 agtctacgaa cttactgatc attggattca tttaaatcac tcctataaaa attatatatg
     4921 tgtattgtaa cacttttcct aatgattata aagtcttttt tcaaaaagac tgtgacatta
     4981 tatagaataa cataatggct aattgatagg atagaattta gatacaaaag taaatctgtc
     5041 ctgaatctgt cctaaggtgc ttttaattta atgctgatta ttattccata tttaaccaga
     5101 tgtgtaagaa atggaatgaa aactaaaaag acaccaaccc gttccggtca gtgtctctcg
     5161 tcaagtggtt aattacatat tgcttacttt ttcatgctca tgtgcttctt tatattttaa
     5221 gcgtgtagct ttacctcccc taatgtgtct ttcagactta tttttaagca aaactttctt
     5281 gacttcgctt gcagctggag ggttcagttt gggaagacgc tctgttacat ccttgtggac
     5341 tgtgcttttg ctgacgccga attttttagc tgtctgtctt acggtagatt cagtacttat
     5401 aatatattcc gcaatctcca tggccctgcg ttctatgtaa tctttcattt ttaaccccct
     5461 ttggcattat accgtcttat ttacattttt atgcgtttat ttgtcctatt agaaccttat
     5521 tttattattc aatggtaata tagtccaaag gatttatttg tttaccgtca acaatcaatt
     5581 caaaatgaag atgcgcgcca tccgatgcct caatgctgac tgttttaccc aaatttccta
     5641 taacctgacc cttttcaacg gtatctccgg ctttgacatt tgccgacact aaattactgt
     5701 acaatgtttt tacattgttc ccatgatcaa taataatttc tgttgctgta atttctgaat
     5761 tgttaacagc ttctactttc cctgctaaag cggattcaat caatagagta tcttttggct
     5821 ttatatcaat tcctttatgt actctccatt caccgatagc ctcatagtaa accaggtcgt
     5881 caattgtaaa ttttctgatt atctctccgc ctaccggtgc attcatggat ttaagatcca
     5941 tttgctgtgt ttttactcca tcatcctcat cttcatcgtc atcatcgttt tctgcttgct
     6001 gaaaattatc aatgatatct tgtacataat cttcataagg atcttcatga acctcgatat
     6061 ctccgtttgg tgtctggcct tctgctaaat cagtccctcc atctccaggt cttgaagtat
     6121 atctccatac tactgctgtt gctaaaagca gtatgaccag tgcaattact aagaagcttg
     6181 tatccttatg cttaagttta ataaagaatt ccttcatttt agtttttgct cttgctaatc
     6241 tgttgttttt catataaacc tccctttttt cagcttcttt attatctgaa aatagtgtgt
     6301 cccatttttt ttatttaata caattttgtt ctaaatattt ctatttttca taaaatatta
     6361 aagaaactta tgataaaggg gttgatttta tgttgaaccg tctgatttat gcttttgttg
     6421 tatttgcaat gcttttggct atcccgaatc tgtctttgaa tttttttgag aaaaaaatga
     6481 atttgaccag tgcagcggta catgagggtg acagttcaaa gtatgcagat gacaaaaacc
     6541 aagaaaaata caccgtggat gtgtatgagc ctgaattaat caaggtgtac aacaacaaaa
     6601 ctaatgatgt gatggttatt gattttgaag agtacttaaa gggagtggtt gcttccgaga
     6661 tgccggcgga atttaatgtt gaagctctta aggcccaggc ggtttccgca agaacatatt
     6721 tattgtatcg cctgaaaaaa tatcctgacg gacagccgga acatttaggg gcacctgttt
     6781 gcaccagcat ccattgccag gtatggagtt caaaggatga attaatccag tcccattacg
     6841 atggatggta tgatgaatac atggataaaa ttgaagtagc ggtagaatcc acaaggggac
     6901 agattttaac ctatgaaggc aaaataattg agcccctgta tcattcaacc agcggcggga
     6961 gaacagaaaa ttcagaggat gtgttttctg cggcagttcc ttatttaaga agcgtggaaa
     7021 gtccatacga ggatgaatcg ccaagattaa acagctctgt taaaattact accggtgaat
     7081 tcattgacaa aattgaatct gcttacggtg atatggacat aacagaaagt aatctggatg
     7141 agaaaattaa actgggtgaa gtcagtgaag gaggcaggat taagacatta ataattgaca
     7201 acactgagat ttcaggacgg gatatgagag cattatttaa cctgaactca actaatttca
     7261 gctttatcca gtccggcagc caaatagaga ttttaactac cggatatggc catggtgtgg
     7321 gaatgagcca gtggggtgct gaaggcatgg ctaacaaggg ctacaattac aaggaaatat
     7381 tgaagcacta ctatacaggt gttgaaattg tgaatatgag gtaaatgaga ttctgcatgt
     7441 gattttgcag tttcggatga ctagactgtg cctatgaatg aaaaatttaa tatacatttg
     7501 atcttttgtg tggtatcatt gtatggaaaa attacagaaa ggaaaataca atgatattta
     7561 tagacgatac agcatataag gaattcaagg agttattgga taatgcaaat gttgactcct
     7621 acaatgtaag aatatctctt gacagggtgg gatgcagcgg accgattttc ggtgtattta
     7681 ttgatgaggc aaatgaagat gatgaggttg aagtaattaa tgagattact ttcatttctg
     7741 aaaagtcaat agttgaagag tatggcggat tcataatagt atccagcgaa gaaaatatgg
     7801 gcagaggtgt tggaatgaag cctgttgtgg ctccgtcccc aagctgtgac gtatgcggcg
     7861 gcggatgcca ttagtattga attgttagaa attgaataca aaaaataatg aatggggaca
     7921 cacaaaatgt ccccatacaa attttttgtt attctgctac atatggcagc aatgcaattg
     7981 atcttgctct tttcactgct cttgttacca ttctttggtg cttggcgcaa gcgcctgtga
     8041 ttcttcttgg taaaattttt cctctttcag ttgtatattt ttttaacctt ccaaggtcct
     8101 tataatctat gtgtgttgcc ttttcttgac agaattggca tacctttttg cggggttttc
     8161 ttctttggct gtatttttca tttttgtcgc ccatgtcttt tccgccctgt ctggaactca
     8221 ttgtactcct ccttccgcta atgtttatta aaatgggata tcttcgtcat ctacaggctc
     8281 aaatatgtca ttgtcctcat cggggaaatc attgaaaaag ctgctatccg gctgtaccgc
     8341 atttccctgt ggtgctgaag actggtcctt tgcaccgaca aattctactt tatctgctat
     8401 gacgtctgta gtgtatcttt tttctcctgt ttgagtagta taacttcctg tctggattct
     8461 gccttgaatt gcgcattgtc ttccttttga aagataatta gcacatacct ctgcactttt
     8521 tccaaatacg gttatgttta tgaaatctgc tgtgggcttt ccctgattta ttgcttcttg
     8581 ttttttttct ccatatatac ccctgtctac tgccaatgta aatttagcaa cagccatacc
     8641 tgttgaaggt atgaatctta ggtccggatc ctttgtcagt cttcctatta atacaacatt
     8701 attcataata ctacctcttc tttatttatt attcttcttc taccgtaatc atatgtctga
     8761 tataattgtc gcttatttta caaattctgt ctatttcatc aacagcatct gctcctgcag
     8821 taaattcaat aaaaacataa taaccttctc tttttttgtc gatttcataa gcaagtcttc
     8881 tatgacccca ctcattaacc ttttcaactg ttccctgtct ttcggttatt atgcttttaa
     8941 ctttttcaat ttctgcattt cttacatctt cctccaagtt agatagtaag ataaaagcac
     9001 cttcatattt tctcatttgg tcacctcctt tggacttttg gcccttgttc gccaagggca
     9061 ggatagctta tacattatag caccagaaaa aatctaattc aagcattatt ttattatata
     9121 ttcagcgttt ttttggcaat agctgcattt tttttcttca ctagtcttaa tcaattcagg
     9181 agcagcctca ttcttattca catagtcatc aagagcctgg tctgcatggt ctttgcatgc
     9241 aaatatttct tcgtactttt ctttcataat tcacctatag ttcatatacc ttgcttgacc
     9301 tgtcacggta ggttaagtca aggcagattc cgggatttac ttcgatagac tcttccaata
     9361 atatggactc tacagtttta taagcaagct cgggaaagtt gttttccttg cttaaatgac
     9421 ccagcaggac aatttctgtt ccccttttta caacgtttgc tattaaccgg cctgcagtct
     9481 cattggacat gtgtccgaat tcacccagaa cccttctttt aagcggccat ggataggggc
     9541 cgatgattac caggtcttca tcatgatttg actctaccaa aagcaaatcc gagtccatta
     9601 tgctgtttgt aattgtcccg ttaaagcagc ctgtatctgt aaccaggctg agctttttat
     9661 gttcgtagaa tatgttgtag cccaccggat gcgcagcgtc gtgggatatg tcaaagggct
     9721 tgattgttaa atctccaatc tgtgtgcagt catccagtat ctttatgtta tggggttcga
     9781 tgtctcccag taaagggctc attgcttccc atgttttttc attggcatat atgggtatgt
     9841 ttaagcgcct tgacattatg cctgctcctt gaatatggtc aatatgttca tgggttatga
     9901 atatagcgtc tatttttttc gggtcctcgc caatatttac aatttcctgc tgaattcttt
     9961 ttccgcttaa tcctgcatcg ataataattg ttgccttgtc tgtcttaata tattggcagt
    10021 ttccgctgct tccgctgtat aatgaacaaa atttaagctt catttgtcct ccccttgcat
    10081 gaacgtccgg ttaatcaatt catattatca taaatgcatt gctttgacaa atactttctc
    10141 atggtgccgg gcatataaca agtaaacaaa ttggtgattg tctnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnggggtat ttatgattat aaagtttaaa tggaaatcat
    10321 tattgatatt tattgctatc atgttaattg tatctgcagt atacttgttt attaatcgag
    10381 ctcagtctgt ttctgcagat gaagaaaaaa gcttcattaa gtgggtggat ttcagagttt
    10441 cttatgacgc catggatagg gctttgaggg ctgatattaa cagcattgac gaagaggtta
    10501 aactggactg gattgaaata ttggcttatc ttggaaccag atacgggggt gatttctttt
    10561 ccaggtataa ggctaaagac atggataatt taataaacag attaaaaagc ggtgaaagca
    10621 tagaaagtat gaccaaggac ctagctcatt ataattacta ctacgaggca tacaaggctg
    10681 tgctggatgg atttgtggga ccctatgaaa tacaggtgaa agatgaaaac accggcgaaa
    10741 aagtctgggt taaaaagtat ggattaaaag cattttctcc cataggtgga ggctaccatt
    10801 acggacatta tgatgacttt ggaaactctc gaaactacgg cttcaggaga gttcatttgg
    10861 gaaatgacct ggtgggttca ataggaaccc cggttattgc tgttgaatca ggacgaatcg
    10921 aagcattagg ctggaatcag tacggcggat ggcggatggg aatacgcagc ttggacaaca
    10981 aaagatatta ttattatgcc cacttgagaa aggatcatcc ttatgtcaaa actctcaagg
    11041 aaggagatat tgtgttagca ggagatgtaa tcggatattt agggatgacc ggatattcac
    11101 gaaaggaaaa tgtcaacaac ataagaactc cccaccttca ttttggaatg cagctggttt
    11161 ttgatgagtc tcaaaaggaa gcactggcag aaatatggat tgatgtatac agaattgtga
    11221 agcttcttaa cagaaacaga tcaccggttg cgtataataa ggaaacaaga gaatttgaca
    11281 ggatttacga cataagattt cacaatatcg aaagaggaaa atggaagggg gacacacaca
    11341 acgaattata gggatacaac tctgttgtca gataccccct gcggggtagt caatggagga
    11401 atgtccataa attaaaccct ttgagtacat tccaaaaaaa acatggggac actcctttaa
    11461 taaaggaatg tccccatgca ttagatttcg cagttttaca agggctgtgc ccttaacgga
    11521 catatatatt tacaatgttg gccatcataa ccgcagcttc tgccctggtt aagcattcat
    11581 caggacaaaa ggcttttatg tgcttgccgg atactactcc gtttgcatat gccgttttaa
    11641 ctgcttgaat ttcttcttct gtaagatttt tcaagtctga aaatatcaaa tttatatcta
    11701 tttcatcaaa tttatggctg ctgactaata tatcctctat caataaaaca gccgtttttc
    11761 gattcaacat ttccagagga tgtacagctt ccagtaatcc cctctcataa atgtctttta
    11821 cataatttag tgcccatgga tgtacattgg tataatcata aaagagaaga ttattttctt
    11881 tgcgtattct gctttgctga ttagcatttt caatataatt tagcgttctg cttaagaaaa
    11941 tacagaattc ttccatagtt acaggtgtat ccggattgta cagggctgcc ttgcttacta
    12001 tttccttgcc gtacatgtcc ataataattt ttctagccca gtgaaatgat atatcctcat
    12061 agatgtatct gccatattgt gtatctcccg tgcgaacaag ccagtttctt tctatccagc
    12121 cttcttttgt ttcatctatg tatactttat accaatagta tccgttcttt aataccggac
    12181 cttcggtaat agttcccagc tggttaacac tgagctggtg gacataattt cccgcaggtg
    12241 catccctcag ataataagcc cccctaatcc cgtctgtcct tacataatca tctttttcat
    12301 aaagtataat gccgccgctg gagcaagata caggcgtagg aaccgtcaga gacctggatg
    12361 gttttgaatc tctgggaaga tatgaaaaat caatattgct tatttttttt ccatattcct
    12421 tttcaattac atcgtaaaca agctgctgat atgtgtattt cttggcatag ggtgaaacag
    12481 gattgttgct ttgagcccag ccgttatatg cccacaaggc aaaataccag ttttccaaaa
    12541 catcgggatc catattgcct acacttgaaa cttgtttgta tgaactcatg ctccatttgt
    12601 taagaagtac atccgcacct gcttcaatat tgtacaatat atcatactta agcctgtagt
    12661 tgtcgtagcc tccgtatata ttgtttatct gcatgagacc gatactggta ccgcatatct
    12721 tgggactgcc gtcactgttg aaatgcttat atacggactc gactcttgct atccccttta
    12781 acagtattgc aggtacacct cttttaaggg caacctcgtc aatcattgcc tcgatttctt
    12841 ttcttggcgg attgacccct gcatacactg ttgtattaaa tatggataat aatattataa
    12901 tcaatataat tctacgattt atggttttca atattaacac ctctctgttg aaattactaa
    12961 taacttcttg tttgataatg taaatattcc cattttattg aaaattatac ctttattttt
    13021 acaatagcat tggtgaatac tcttaaagtc aaaaaacaag aagggacagc actccttttc
    13081 agaagtacca tccccatcag attgcgaagt tatgcgggtt agggaaagaa aaatagggtt
    13141 agggcatcag tttttcagaa actttatcca ttattactgc agcttccgct ctggttaaat
    13201 atgagtcagg gcagaaaccg gatgaggtct tgcctgtcat tatgctgttg gtgtagactg
    13261 ttttaacagc agcaatttcc tcttcactta aactgtttat atcagtaaat atcgtttcca
    13321 tatccaagga agaaaactct tcttttattt caaataaaca attaataatt aaagctgctt
    13381 cttttcggct caggctttcc aagggagcaa gcaaatcttc ttcttcaacc aatcccagat
    13441 tataaacatc cgctatatat tcaagagccc atgggttaat actttctaca actgtcgtca
    13501 aaacaggtgc tgccccttgg gtaactattt cagattccaa tgacttatca ttttccattg
    13561 aggacttgta atcttcattg gagggttctt cgtctttcgt ggacataagt tcttctgatc
    13621 cggcattttc gtattgggca taatctagcg tcttgcttaa tagaattaag aactcttctt
    13681 tggaaataaa attatcggga ttgaagtatt gtgcttggct gacaatatcc ttgccgtaaa
    13741 gctccattat tatctttctt gcccagtgga aggatatatc ctcgaataca tatctcccat
    13801 actcaatatc accggttcga agaaggaagt ttctttctat ccagcctgca gtgttttcat
    13861 ctatatatac tttataccag tagtatccgt tttgcagcac aggtccttca tcaataacag
    13921 ctaattgatt taaggaaata tcatgtatat atcttccggc gggaccgtcc ctcagataaa
    13981 acttttctct cataccgtca gttcttacat aatcaccctt atcataaaaa attatgtcac
    14041 cgccattggt gtacatgggt gtaggcacaa ccagggatct gcttggtttc cccgttgaag
    14101 gaagaagtga aaaatcaata ttgtgtactt tcccgtcata ttccttttct ataacatcat
    14161 aaatcagctg ctgataggta tatttttttg cataggatga aattgtgttg gggttgttgc
    14221 tgctggccca tccattgtaa gcccataggg caaagtacca gttttccaga acgtttgggt
    14281 ccatatttcc aacacttgga acaatattgt atgaactcat gctccattta ctcagaagaa
    14341 catccgcccc tgcttcaatg ttgtacatta tgtcatattt aagcttatct gtgtcataac
    14401 cgccgtttct attgttaatc tgcatgaggc cgatgcttgt acctgttatt ttaggactgc
    14461 cgtcttggtt gtaatgctca taacaagatt caacccttgc tattgctttc aaaagaatgg
    14521 atgggattgc ccttttttcg gcgaccctct caatcataaa ctctaattct tccctggatg
    14581 gattaactcc tgcatatgca aacgtattca atgccaatac aaaagtgaat attattaaaa
    14641 taacttttcg ggcagtgctc ttaaatattt tcatatttgc cttctccctg attagatttt
    14701 aattctagcc gatatttatc tgtatacggt caattatatt taatatctcg ctttttgctt
    14761 tttccaatgc ttcttttgat ttttcgcggc tttttccgtt aattgaaaaa tacaccttca
    14821 gcttgggttc tgttcccgaa ggtctcaata caagccagga cccgtcttca aaacaaaact
    14881 tcaaaacatc cgactttgga taatttaatt ttaccgtagt ccctttaata aggtccagtg
    14941 aaatactcat tttataatca ttaatcgctg gaattttttt gcctgccagt gaatatagtt
    15001 tgttttctct gaaatattcc ataattgctt tcattttgct ttgtcccgca aggcctgaaa
    15061 agcttaatga aatagtttct tctatataat aaccgtgctc ttcatatatt tcatctaaaa
    15121 catcgattaa tgtctttccc agcttcttat aatatgcagc catttcacaa atgagcatgg
    15181 aagcaatcac tgcatcctta tctcttacaa aggttcctgc cagatagccg tagctttcct
    15241 cataaccgaa gaggaaatta tgttcttttg tttctttaaa cttctgtatc aattcagcaa
    15301 tatatttaaa acctgtcaaa gtgtcaaata cagtaacatt atatttacct gcaatgcagt
    15361 ttgcaacttc gctggttaca acagttttaa caattgccgg agattcggga gtccttccct
    15421 gttcattcag agattcaagc aggtaattca aaagcagcgc acctgtttga ttcccgttta
    15481 aaggaacata ttctcctttg tgctttacca ctgctccaac ccggtcgcag tcggggtcgg
    15541 tgcctataat tatatggccg tctatttctc ctgctttctt tattgccagt tcaaaggagc
    15601 ttatatcctc cggatttggt gatttgacag ttgaaaactg agaatccggt ttttcctggt
    15661 cttcaacaac aaatacattt ctgtatccca tttcatccag tactctcctt accggcttgt
    15721 tgcctgtccc atgcagggga gtgtaaataa tttttatatc cttgtcaata tcatctctta
    15781 agcttaaact ctttatttca tttatgtaag atgtatccac atcatttcct gccatttgaa
    15841 tcattgcatt acctttaaaa actgataatt ttatgtctgc cagtttaacc ttgttaattt
    15901 tttcaatgat tgtgtcagca tcctgtacaa cctggcctcc aaattcatcg tataccttgt
    15961 aaccgttgta ttccggagga ttatggcttg cagttaccac gacccccgcc ttgcagttaa
    16021 aatgcctcac tgcataggat aattcgggtg taggccttaa atcatcgaaa agataaacct
    16081 ttatattgtt tgctgcaaac acgcatgctg ttttttccgc aaattccctt gacatatttc
    16141 ttgagtcata tgcaattgct gctgaaattt cttcattttt gtgcttctca attaagtaat
    16201 ccgctattcc ctgggttgct tttgcaacgg taaccagatt cattcgattg gtgcctgcgc
    16261 caattttccc cctcatgcca cctgtaccga actccagttc cctgtagaac ctgtcttcaa
    16321 tttcgttatc aagcttttca atttccttta actctttttt taattcatca tctacgtatg
    16381 gactgtttag ccatatttta aatttttcat tgtgcatgtc cccacccctt attctaaatt
    16441 tattgatttc aaatattttt taaaatcttc tctcagctca tcacttttaa gagcgtagtc
    16501 aacagttgct tcaagaaagc cgatcttgct ccccacgtca tatcttcttc cttgaaatat
    16561 gtaggcatac atttcttcag tagtgcaaag ttccctcaac ccgtccgtaa gctgaatttc
    16621 tcctcctatg cccggctttg tattttcaag tatatgaaat atcttaggag ttattatata
    16681 ccttcccagg attgctatat ttgtaggtgc actttccttg tgaggttttt caaccaaatc
    16741 attcaccgta tatattctgt cattaattct ctcacctgac actatgccgt atttatgcac
    16801 ctggtcccat tctaccggct ggactccaag aattgttgac ttgtattctt caaaaacttc
    16861 cagcatttgt ttaagacagg gtttttctga atccacaaca tcatccccta agaggactgc
    16921 aaaaggctca tctcccacaa aggccctggc gcaatatatt gcatgtccca gtcccaaagg
    16981 gtctttttgt cttacggtgt gaatatttat cattctggaa attcctttga tttcttcaag
    17041 aagctcttcc ttattggcat cctttaagat atattctagc tctactgccc tgtcgaaatg
    17101 ctcttcgact gaacttttgt ttcttgcggt aatgatcaat acatcttcaa ttccggaagc
    17161 agcaatttcc tctactatgt actgtattgt gggtttatcc acaatgggca gcatttcctt
    17221 tggaattgct ttagttgcag gcaagaatct ggtaccaagc cctgctgcag gtataactgc
    17281 ctttctaacc ttcatagcaa acctctattt tcactttcta attaatctga ttaatcgcag
    17341 ccttagtgcc gtccttaaaa ccggcgaaga atctatccta cgttgtaatt tgttttaata
    17401 tgttcagaaa ttaaagcatc ctttagaaca tccatatgct ccagggactg ccctgttccc
    17461 ttggcaacgc aggatacggc gtcttcggct atggttacct tcaagccggt tctcttttcg
    17521 agcagtttat ccattccgtg aatgagacat cctccgcctg tcatgacaat gcctttctcg
    17581 cttatatcgg cagccagctc cggcggggtt ttttcaagga ctgaatggac tgcatcggct
    17641 attgtagtta ttgtttcttc catagcttcc agcgaatctg aagatgaaat agtcagattt
    17701 accggcagac ctgttaccag gtttcttccc cttacttcca ttaatctttc ttcttctatg
    17761 ggaaatgctg ttcctatttt tattttaagc tcttctgcac ttcttaagcc aatcataaca
    17821 ttatatttct ttctgacatg ccttacaatt gcgtcgtcac acttatcccc tgcaaccttg
    17881 atagacctgc ttacaactat tcctcccaat gatataacag caatatccgt tgtacctccg
    17941 cccatgtcaa taaccatatg ccctgaaggt tttgttatat ctagtcctgc ccctatggca
    18001 gcggcaacag gctcctcaat caggtatgtc ttttttgccc ctgcgctgtt gcttgcttca
    18061 agaaccgctc ttttttcaac ctctgtcaca ccgcttggca cacagataat aaccctgggc
    18121 ttaaccagtg aattgccaac cgcttttttt ataaaatatt tcaacatttt ctccgtaatg
    18181 tcaaaatctg atataactcc atctcttaag ggccttatgg ctacgatatt tccgggtgta
    18241 cgtccaagca ttttccttgc ttcttctccc acctcaagaa ttttgttcgt attaatgtca
    18301 attgccacaa cagacggctc attaattact attcccttgc ctttaatata aaccaatacg
    18361 ctggctgttc ccaggtcaat gccgatatcc attctcagag acattttatc ctccatgtta
    18421 ctttcttctt agtatgctta actgtaaaac atttaatgat aaaaattata acatacaacg
    18481 atatttaatg caatttatct tattttttaa gcagcttttt aattttttga accggatcca
    18541 ataattcgct taaagaattg tcataattta atgtatctat aatatttgca atatactttg
    18601 acatatccac ctcagcgtac cagggcttat cgaacagctc cggttttctg taaatcatat
    18661 ttgtggtaaa cacctttgat aatatgccgt cccggtatgc ctcgtcgaat ttggaaattc
    18721 cttcagtaaa caatccgaat gtggcaaatg catatacatt ccttgcgctt ctttctttta
    18781 acttgcctgc aacatcaatc aacgaatctc ctgaagcaat catgtcatct acaataataa
    18841 catccttccc ttccaggttt cctcccagga attcatgact taacacggga ttcttaccat
    18901 taactactac ggtataatct cttctcttat agaacattcc taaatccagc tccataaccg
    18961 atgaataata aatacatctt gtcattgcac cttcatcggg agaaattatc atcatatgat
    19021 ccctgtcaat tatagcttca gggactgttc ttacaaatgc ctttatcatt tgatagtagg
    19081 ggtgaaaatc ctcaaacccg tgaagaggaa ttgcattttg aactctaggg tcgtgtatat
    19141 cgaaggaaat tatattttca acccccatat tgacaagctc ctgtaatgcc agggcgcagt
    19201 ccagggactc ccttgcagct cttttatgct gtctgccttc atatagcatt ggcattataa
    19261 ctgttattcg ctttgccttg ccgttcaatg ccgcaattat tcttttcaga tcctgataat
    19321 ggtcgtcagg gctcatgggt acttctgtgc catacatatt aaaattaact ccgtaattaa
    19381 aaacatctga tattataaat atatcatgtc ctcttacagt ttcgtataaa actcccttcg
    19441 cttcaccggt gccaaatctt ggacaggcag cttttataag aaatgtacct atgggacctg
    19501 aaccatttga atatataaca ccattggcat cctgcctcca gtcctttagg tagtagtcta
    19561 tccttgaggc tatgtcttcg cagcctttca ttgcaataat ggatagtctc ccgaacgggc
    19621 tgggttcttc gaacgtgaaa tcatttatgt tcattttctc ctcctgttaa ccttatcatg
    19681 ttacatttta tcaaaaaaat attaattgtc aaaagattat ggttctcttt ttattaaatt
    19741 atcttaacaa taaaaaattc atagtcagcc tatcctgtgt gttctaggat tctgccgact
    19801 atgaattctt gcaatgcacc taaaaataca ttttagcaat tgtctctgac aagacttcat
    19861 atgtaaatgg tttttcaata atagtaagcc ctcttctcat taattcttcg tattctgcct
    19921 gttggacaaa accggttata aaaatcgctg ctgcctcttc attgataaga agaatttttt
    19981 catatagttc tctgccgttg agttcaggca tcattatatc caatacacaa aaagatattt
    20041 ttttatattc agcttcataa tatttcaatg actcaagagg agaattgaat tttaccacgt
    20101 cataacctaa atccttcagc atttcggctt caatatccaa aacatttata tcatcatcaa
    20161 ccagcattac gatattgtta tctttatctt ctattttctt tttttcatat gcttccgttt
    20221 tttccagtgc cggtataaaa atttcaaacc ttgtcccatg gcccggactg cttgtaacat
    20281 tgagataacc ggtatgggat ttcacagtcc cgaacactac agacaaccct agcccggttc
    20341 cgtttgattt attttttgtg gagaaatatg gttcgaatat tttcagcagt atatcttcgt
    20401 ctatgcctga accgttatcc tctatataaa ttttaatata ttgacccggc tgaattattt
    20461 caccatgact taaagccatt tcagaaagaa ttacagtatc cgttgttcct attttaatca
    20521 tgccgtaatc tttaattgca tcctttgaat ttaatatcat atttactatc atgctttcaa
    20581 gctggcactc atcccctagt ataacttcat catttgcttc atagctgtat atcaaatcga
    20641 tttctttaga tagaataaat tccatcattt tgtaagtgtt atccattatg ttttttaagt
    20701 ttacaggttt atttgtactg ctttttttct tggaaaacat aagaattttt tttgtaagct
    20761 ctgctgcact ttgcgaaata tggattattc tttcggcata atcataaatt ctattcaaat
    20821 catctgtttt ctgtatcatt gtagcattgc cgattatact catcagctga ttgttgtaat
    20881 catgagctac tcctcccgca agctgaccaa gggcagccat cttttcattt tgtctttcag
    20941 ctattatttt caattcctcc cttccaactt cttcgtttga agcattgtat ttcatatct
//
LOCUS       DAOPUT010000018        20587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_2600_length_20587_cov_19.576076, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000018 DAOPUT010000000
VERSION     DAOPUT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 20587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20587
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2600_length_20587_cov_19.576076"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1870)
                     /locus_tag="PKK75_03190"
     CDS             complement(2..1870)
                     /locus_tag="PKK75_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNZ82630.1"
                     /translation="MKGYKDCIINVNLTGGIIKKEKLNRDYIQKYLGGEGYGIALLWD
                     EMPHNAAPLSPDNILSFNTGPMTGTPTPSASRTSVCFMSPLTNTIGASNVGSHFGAEL
                     KFAGYDTVSFKGKSEKPVYLYIDNDKVELRDASGFWGMDTRQTTDAIKEETGKEFKVC
                     CIGQSGEKLSKLACIFSDDCFAGRGGAGAVMGSKNLKAIAVRGTGKVEIADKDAFMAA
                     YKDAYKQLRDEAYTWGPVHGYGTPSWVGGSHDIGILPTRNFLQNQFDKIEGVLPYQLW
                     NNTDKYSVRRRTCYACQLGCHKYVVMGDTEVGEMEYETLAAYGPRCGVDDYESICMAG
                     YYTTIYGFDTISGGATISAIMEWYEKGIITKDQTDGLEMNFGNGPAMAEMTRKMGLRE
                     GCGELYADGSFEAAKRIGGKAMDYVMAVKGMEISATDPRGASSMTIAFGTSERGACHM
                     RPYAASVDAFGYVFPDLDINEPRDPFNDAEDKTWLKAIKEYFVVTNLAGICDFNVINL
                     EMKASTIAELYTAITGIEMDKLQLLKTAERTIALERALNYERGFTRKDDRLPKRFMTE
                     PAVGGPPKGRVADMETALDKFYEACSYDMEKAIPTPEKYEELGMEDVAEKIYSLNY"
     gene            complement(1882..2295)
                     /locus_tag="PKK75_03195"
     CDS             complement(1882..2295)
                     /locus_tag="PKK75_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ82631.1"
                     /translation="MKPGVIKINVNKSVCQGCRTCEAVCSLVHTDAVSPQTRGIQVKE
                     LDELGKFKLTVCQQCSDMQCGQACPVGCINRNSYSGAVEIQEGCIGCGACAEACPIGA
                     IVMVNLDGETKPYKCDLCGGLPQCVPACPRQALSW"
     gene            complement(2315..2545)
                     /locus_tag="PKK75_03200"
     CDS             complement(2315..2545)
                     /locus_tag="PKK75_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNZ82632.1"
                     /translation="MKIRVKLKGILASSAGFREREIEMPEGTTIAEALNIINLPVSGE
                     WTKSSVNGRLRGKSCVLNDGDELLFFPVGGAG"
     gene            complement(2791..3621)
                     /locus_tag="PKK75_03205"
     CDS             complement(2791..3621)
                     /locus_tag="PKK75_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNZ82633.1"
                     /translation="MDRLTIGQMSKINNVSEQTLRYYDRIGILKPIEVDKFNGYRYYS
                     MKQCARLDMIQYLKSLGMRLKDIEVQLISCDTKLIKRFLEEKSRNIDAEIEALKNQKN
                     ALQRAVRNYERYESSPPDGTIALEYIEKRWIYSYNMSTNFYDYGIEIYEEQLRKLKED
                     MERNGLRQTYFCNAGSTVAYEQIINRDFISSGLFVFVDENLPENLIKPIKAGTYLCIY
                     CNRFEQEIPYANRLLDEISKRNYTIDGDYICEVITDLPNLTDAERGMFFKLQIPVKIT
                     "
     gene            complement(3789..4547)
                     /locus_tag="PKK75_03210"
     CDS             complement(3789..4547)
                     /locus_tag="PKK75_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HNZ82634.1"
                     /translation="MESIQKVDLTRVKPYGDTLNDGMVQMSFTLPVPYGDEANEAAKQ
                     LAEKMGFDDPAVVFSKDLGVGYTYFVMYGKCTHTVDYTVIEVPKVDMEFMEREEVEEY
                     IKENIKRDVVIVGACTGTDAHTVGIDAIMNMKGFDGHYGLERYKGIEAINMGSQVLNE
                     ELIAKAVEVKADAILVSQVVTQKDVHIANLTQLVEMMEAEGLRDKMILVCGGPRINHE
                     LAQELGYDAGFGTGSYANHVATFVVHQIVKRNLI"
     gene            complement(4547..6103)
                     /locus_tag="PKK75_03215"
     CDS             complement(4547..6103)
                     /locus_tag="PKK75_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HNZ82635.1"
                     /translation="MESKLNLNPQLIQSARTAAKNIADDVQNFIDKNTTVTVERTVAR
                     LMGVDGVDEIDKPLPNVLIDAIKEGGGLSKGAAFWIGNAMVNTGKTPQEIAEKTSSGE
                     IEITKLPIADENKIMDAVYETAKQTVERIRNNRARRDEYLNTIGEGRKPYLYVIVATG
                     NIYEDVLQAQAAAKQGADIIAVIRTTAQSLLDYVPYGPTTEGFGGTYATQENFRIMRK
                     ALDEVGEEVGRYIRLCNYCSGLCMPEIAAMGAMERLDVMLNDALYGILFRDINMQRTL
                     VDQYFSRIINGFAGIIINTGEDNYLTTADAYEEAHTVLASQLINEQFALKAGIPEEQM
                     GLGHAFEMNPMLENGFLYELAQAQMAREIFPNAPLKYMPPTKYMTGNIFRGHLQDAMF
                     NFISIWTHQGLQLLGMPTEAIHTPHLQDRMLSIENAKYIFNNARDIGNEVEYKKDGII
                     QKRAQEVLAKAEELLRDIQREGIFDTIEKGKFGGVKRSFTGGKGLAGVVEKDEKYFNP
                     FIDLMLGGVK"
     gene            complement(6148..7743)
                     /locus_tag="PKK75_03220"
     CDS             complement(6148..7743)
                     /locus_tag="PKK75_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ82636.1"
                     /translation="MNSFMTENTVRNINFDYIFNEIKPITEYGIKAKLEAKPYIKGQE
                     ADLKTEFNKIRAFIENRQRREIIDILRHIKNILETIQRARSNQVLDEVELFEIKNFLI
                     LVEKLDKVLRGISDNDIELLQLTPLPRLYEKLDPAYEKLSTFYIYDEYSQKLKEIRNK
                     KRQIEKTVSLLKKKIKEEIQEKHSIKLNLKDEITISKSQKERIEELNKEGGLRVSGEN
                     YLNIIYKLKNTDEIDEYEKFYEELTFDEDEEEYRIRQDISGSIKKDSHILQENTKIIG
                     QIDYLIAKANYAQKTRSVEPTITDELEITIKGGRNLKLEKTLKDKHLEYVPIDLMLNK
                     NVVCITGANMGGKTVTLRMTGQIAAMASFGMFVPCDYAKLCLFEHIFISAGDDQSIEK
                     GLSTFGAEIVNLKEAIKNSDRRCLILIDELAGGTNPKEGYAITKAVVNYLKNKDCITL
                     LTTHYDNVANDKDIQNLQVKGLRLPDEADLIKIDNIGQVQKYMDYRLIEVKYSSDIPK
                     DALKIARMAGIDGEIIKDAERFL"
     gene            complement(7740..8768)
                     /locus_tag="PKK75_03225"
     CDS             complement(7740..8768)
                     /locus_tag="PKK75_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82637.1"
                     /translation="MRLIQTIKQNNYETVSIIGLAKNAGKTVTLNYLIEEAENLNIKT
                     GITSTGRDGENTDLVTQTKKPAIMVTEGMYAATAKKTLMLSNARTEILETTGISTAMG
                     EVIIVRVRQKGNIQIAGPVSAKDMKYVAGRLKHYGAEVVFIDGAIDRKAVSSPVITDA
                     CVIATGAVLSRDMKKVLEKTAHAVECYSLPGTDVHVKNIVRKINKTCIISEDGNILVP
                     DIKTSITGGRKISELIDGKTSHIFIKGALTSALLKELWGNIYLRGINLIIEDGTKIFT
                     DINVWNEMRRKGLKVEALDTINVLAVTLNPVSPEGYFFDSEVLKENMKKTLPGINIVD
                     VVSGGDED"
     gene            complement(8826..10076)
                     /gene="ablA"
                     /locus_tag="PKK75_03230"
     CDS             complement(8826..10076)
                     /gene="ablA"
                     /locus_tag="PKK75_03230"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNZ82638.1"
                     /translation="MAYYNEIELWKDVKPEEWNDWRWQVENRIDSVEKLKKIINITKQ
                     EEDDINKVLEKFRMGITPYYAAHMDKDDPRHPIRMQAVPTFAETHVSPADMLDPLHED
                     SDSPAPGLTHRYPDRVLFLITDQCSMYCRHCTRRRFAGQSDDEVGMENIDKCIEYIRK
                     TPQVRDVLLSGGDCLLVSDSKLEYIIRKLREIPHVEIVRLGSRTPVVLPQRITDDLVN
                     MLKKYHPIWLNTHFNHPSEFTPESIEALRKLADAGIPLGNQSVLLRGVNDCPHIMREL
                     VHKLVMNRVRPYYIYQCDLSLGIEHFRTPVAKGIEIIESLRGHTSGFCVPTFVVDAPG
                     GGGKTPVGPDYVLSRSAHKLILRNFEGVITTYTEPDIPELECVCDVCQGRKEDPLTGV
                     AGLLQGNDLALEPTKLARRDRVHH"
     gene            complement(10165..>10602)
                     /locus_tag="PKK75_03235"
     CDS             complement(10165..>10602)
                     /locus_tag="PKK75_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HNZ82639.1"
                     /translation="CLYEAKKKAGPTGKVICWAHRQASLNTVKELGLADVLISGDATN
                     AIEVYEKVMEATDGRLCDLVISCVSRPNCEMSAILAAKDEATVYFFSMATSFTKAALG
                     AEGVGKDINMIVGNGYTKGHSETALQVMRESKGLRELFTKLYA"
     assembly_gap    10603..10702
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10952..11758
                     /gene="murI"
                     /locus_tag="PKK75_03240"
     CDS             10952..11758
                     /gene="murI"
                     /locus_tag="PKK75_03240"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNZ82640.1"
                     /translation="MDNRPIGIFDSGVGGLTVMREVMEQLPYEDIIYLGDTARIPYGS
                     KSEQTIKKFAHQCSEFLKNKDVKTIVVACNTASSVALDQLIRDFDIPVIGVIEPGARS
                     AAEATETGRIGVIGTTATINSYAYQGKIMEYLNNAEVIGIPCPLFVPIVEEGWEYSQV
                     AMLTAEKYLTELIEHDIDALVLGCTHYPILRHIIKKVLGPKVKLVNPAFETAKDLKKL
                     LAEKDLFNENHSKPKYEYYATDAPERLRRIGGNFLKKEIDALYQVAIDNL"
     gene            11773..12192
                     /locus_tag="PKK75_03245"
     CDS             11773..12192
                     /locus_tag="PKK75_03245"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HNZ82641.1"
                     /translation="MEDVKIRINTTQTIDEAGNEDVIELLTEAKLEKLKDCFIINYDE
                     SEITEQEGSRTRLKVYKDKMLMTKVGVYSSKMEFQQGKKYKNLYSTPYGNFDLEYYTV
                     LYDNKLNEKGRGRIYTEYLITFADSDESYNKLNIDIM"
     gene            12259..13656
                     /locus_tag="PKK75_03250"
     CDS             12259..13656
                     /locus_tag="PKK75_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ82642.1"
                     /translation="MEIKYFDALIEAIELINDGIQIIDASGKIVYHNPSSKELDLIDA
                     EKTIGRHILEVYPSLTFETSTIMNVLKNGKPIYNVEQNFVNYKGDKISTLNTTLPILY
                     NNKVIGALEVSRNMTKVRELSERIVNLQRELYEAGQGAEKTSKPLAKFNFLDIIGQSK
                     EILKLKSLGLKAAMTDSPVMVAGSTGTGKELFVQSIHNSSDRKFKPFIAQNCAALPAN
                     LLESILFGSAKGSFTGAENRPGLFELADGGTLFLDEINSMPLDLQTKLLRVIQDGRVR
                     RVGSSRTTDVDVRIISAINTNIGTVVETKQIRQDLFFRLNVITMVIPDLSKRVEDIPL
                     LVDHFIKKYNEKCNKYFNGISRDVLDIFLDYSWPGNVRELEHAIESAISLYDGEIIRE
                     EHLPFQFKNYQLNGSSLDTGTIQHLNTAVEKVEREVILSALEQTDNNITKAAKLINVP
                     RQTLQYKIKKLEILF"
     gene            complement(13724..14824)
                     /locus_tag="PKK75_03255"
     CDS             complement(13724..14824)
                     /locus_tag="PKK75_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HNZ82643.1"
                     /translation="MKNIRIIIITVISSILLTGCWDSIEINKRDYIFAVGIDKNDKNL
                     TFTSEIPKINEGSEEQRLVYTKDSENFADFYATSYLHSEKAISDRLMQVIVLGEDVAR
                     DSETVKRIFDEIQRSPQMNRRVKICIAKGKAQDIINTEIPSNPIVGRFLSEMLIKLKR
                     ENYQDVYTFDEAILNLGKTGNTMIPVVEVNEKSLKIERAGVFKEYELKGFLEPDEVAA
                     VMVVLNPNKAKIRNVNIMVEGTDVSLGVVNIIMTTDMNISGDKLTVDYYATLNSYIDS
                     FIIGSNKLEDQSYLNKIKEEAIKSILTVSESALDKLQNVYRTDLLKIKEGLFKYHKKD
                     YEKISGKYDEVFAAADINIHLDMVIKSTGLVK"
     gene            complement(14977..16065)
                     /locus_tag="PKK75_03260"
     CDS             complement(14977..16065)
                     /locus_tag="PKK75_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HNZ82644.1"
                     /translation="MFEQKITPYQNLSILILVSFAFQSILMPLLLSEIDGPVGWMTII
                     IAAFVLYFALKPINKMMLKYKDDSVLSIINKIFPKFLSRLTGIYYILMFLTANSILMK
                     DFAEQIKLMMLFRTPLGTIIIVILLTASYATKKGIKTIANIASIALLTAFIPYLLIII
                     FSTYYADYTNVFPIYPFDINGVVRSVSGSVLGFFGFSILLFSNSMVTVQENNRFISRK
                     FILISSILYIACYLLIIVKFGIKESVHLVWPFISVMKYVNIPGFFFESTEIVGLCFQI
                     IVTFSCICIIAYFTNMAMQETFKTRENGYFVFIQIPVLYIMAVVLPGMYMMFPYIQWP
                     IVVFSSLNLLIPLTAVLVDVRSKKAKNK"
     gene            complement(16065..17570)
                     /locus_tag="PKK75_03265"
     CDS             complement(16065..17570)
                     /locus_tag="PKK75_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HNZ82645.1"
                     /translation="MSIYQDLANIKVDRDLKKNENVLKETFRDANDIVFRNMKVGQNE
                     KLKILLFYVDGMTTKDSISEFAIQTLMESIDLESLEKSNGIELENKVLQTNIAITELG
                     VLNTLQECVDKALSGETILFINTCSRAIWLSSRGWQLRGIQEPAAETLIRGPRDGFIE
                     TMKINITLIRRRIRDPRLKVKYMQAGTRSKTDLAIMYIEDLVNKTVLEEVEKRITNID
                     IEAVLESSYIEELIEDDVYSPFPQVENTERPDAAASALLEGRVVIAVDNTPTVLMAPA
                     TFVSFMQSSEDYYERWMLACLIRMIRYIALPIVILLPAIYVAVTQFHPNMLPTQLALY
                     VAASRANVPFPPYFEALLMELVMELVREASLRITTPVGSTIGLVSGLVIGSASVEAGL
                     ITPIAVIIVALTALASFAIPSYNFSTSLRMIRFGFIISASFFGLFGISIGLCILIIHL
                     CTLKSFGVPYLTPMTHFVERRSDLKDTIIRPKIKTLVRKPKYLQVEKENGK"
     gene            complement(17579..17779)
                     /locus_tag="PKK75_03270"
     CDS             complement(17579..17779)
                     /locus_tag="PKK75_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82646.1"
                     /translation="MSLINHIKPVINIDVIIVFLIISYILIFKVSKDFKRKNYHRDYK
                     IVRITGIIYGLIAIGAIIAKNI"
     gene            complement(18014..18712)
                     /gene="spoIIR"
                     /locus_tag="PKK75_03275"
     CDS             complement(18014..18712)
                     /gene="spoIIR"
                     /locus_tag="PKK75_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HNZ82647.1"
                     /translation="MSYYKKIFISAIFVILAIGIAYTVEAYEGISRLNGKVIRLHVVA
                     NSDTLEDQQLKYKVRNRIIATFNEEFEKIDSKEESENIVLEKIHQIRCEAEETVKEEG
                     YNYEINVYYGNYKFPRKMYEEIVLPEGYYDAVRIEIGKAEGSNWWCVMFPPLCFVDFG
                     KTRDAAVFDIETEKRLQEVLTLDEIDTIRTKRGIENIKLKSKIFEFIEKGRVEKTGIY
                     TFAGSPDYAVSLSK"
     gene            complement(18887..20557)
                     /locus_tag="PKK75_03280"
     CDS             complement(18887..20557)
                     /locus_tag="PKK75_03280"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ82648.1"
                     /translation="MPLMKNDLNLGFIITDDKFNVLYCNEYFKTNIIKTDILNKKITM
                     FFQNINVDMDKNLYSVRYIDNSEYYVIHNNFKLYNHELYSFFFYDFTIGSELQKQIER
                     LNSQQYLYNEMLNKLKDGIYITDELGTTIYVNDAFLNLSGLTREQIIGNTVYDLRKSD
                     ILPNSCCAKVIETKGPVSTINHYYKGQRCLVSGSPIFDEKGNLSKTIAVIRDVSELDV
                     LMKTITKEKNLFIQSDYNVKTESSHDPEEIINNIKMKNIYEKAKKISKVDSTVLILGE
                     TGVGKDFIATYIHSISSRNKGKFIKINCGAVPEHLLESEFFGYEEGAFTGAVKGGKKG
                     LFEEANGGTLYLDEIGDMPYTLQVKLLSAINDKMFYRIGGTSPIEFNARIIAATNHDL
                     KELVEEKKFRADLYYRLNVVTFVIPPLRSRKEDIIPLSTQFLEFYNNKYGKNCYISSS
                     CLQSFLIYEWPGNIRELKNIIERLVLMADGAEIGTDLFREHLILPGGTDPAELFVTGT
                     KTLKEKTEEYEKEIIESTLAVSKNMKEAAMRLGIDISTLVRKKQKYSL"
BASE COUNT         5684 a         4275 c         3501 g         7027 t
ORIGIN      
        1 tctaataatt caagctgtat attttttctg ctacatcttc cattcccagt tcttcatact
       61 tttcaggtgt cggtattgcc ttttccatat catatgagca ggcttcatag aatttgtcga
      121 gggctgtttc catatccgca accctgcctt ttggaggacc cccaactgca ggctcagtca
      181 taaaccgttt aggcagcctg tcatcttttc ttgtaaaacc tctctcataa ttcaaggctc
      241 gttcaagagc tatggtgcgc tcggcagttt ttaacagctg aagcttatcc atttcaattc
      301 ctgttattgc agtataaagc tcagctatgg ttgatgcttt catttcaagg tttataacgt
      361 tgaaatcgca tattcctgct aagtttgtca caacgaagta ttctttgatt gctttcagcc
      421 atgttttatc ttctgcatca ttgaacggat cccttggctc gttaatatcc aggtcaggga
      481 atacataacc gaaggcatca acagatgctg cataaggacg catgtggcag gcacctcttt
      541 cgcttgttcc aaatgcaatt gtcatggaag aagctcccct ggggtctgta gcagaaattt
      601 ccatgccttt tactgccatt acataatcca ttgccttgcc gccgattcgt tttgctgctt
      661 cgaaggaacc gtctgcgtac aattctccgc agccttccct caatcccatt tttctggtca
      721 tctcagccat tgccggaccg ttaccgaagt tcatttcaag accgtctgtc tggtctttgg
      781 tgattatccc tttttcatac cattccatta tggcactgat tgttgcgccg cctgaaatgg
      841 tgtcaaatcc gtatatagtt gtataataac cggccatgca tatgctttca taatcatcaa
      901 caccgcatcg gggtccgtag gctgccaatg tttcatactc catttctccc acttcggtat
      961 cgcccataac tacatatttg tggcatccaa gctgacaggc atagcaggta cggcgcctta
     1021 cggagtattt gtcagtgtta ttccacaatt gataaggaag gacaccttca attttatcaa
     1081 attggttttg taaaaaattt cgtgtaggca gaattcctat atcatgggag ccgcccaccc
     1141 atgagggtgt tccgtatcca tgaacaggcc cccatgtgta agcttcatcc ctcagctgct
     1201 tataggcatc cttgtatgct gccatgaatg catccttatc ggcaatttca acttttcccg
     1261 ttccccggac agctattgcc tttaaatttt tgcttcccat cactgcaccg gctccgcctc
     1321 ttcctgcaaa acagtcatcg ctgaaaatac atgcaagctt tgaaagtttc tctccggact
     1381 gacctatgca gcatacctta aattcttttc ctgtttcttc tttgatggca tcggtggttt
     1441 ggcgggtatc cataccccag aacccggatg catctcttaa ctccacttta tcattgtcaa
     1501 tatacaagta gacaggtttt tcagacttac ctttaaatga aaccgtatca taacctgcaa
     1561 attttaattc tgctccaaaa tggcttccta cattggatgc gccaatagta tttgtcaggg
     1621 gagacatgaa gcatacgctg gtacgggacg cagaaggggt gggcgtacct gtcataggac
     1681 cggtgttgaa actgagtatg ttgtccggag ataaaggggc tgcattatgg ggcatttcat
     1741 cccacagcaa agctattcca tatccttctc cccccaggta cttttgtata tagtcccggt
     1801 taagcttttc ttttttaatg attcctccgg ttaaattaac attgattata caatctttat
     1861 aacctttcat cttcacacct cctaccatga caatgcctgt ctggggcagg caggtacaca
     1921 ttgcggaagt ccgccgcata aatcgcattt atacggtttt gtttcaccgt ccagattaac
     1981 cattacaata gcgcctattg gacatgcttc agcacaggca ccgcatccaa tgcatccttc
     2041 ctgaatttca acagctcccg aatatgagtt tctgtttatg catcctacag gacaagcttg
     2101 cccgcactgc atgtcagagc attgctgaca tacagtcagt ttaaatttac caagttcatc
     2161 taactctttt acctgtatac ccctggtctg aggagataca gcatctgtat gtacaagaga
     2221 acatacagct tcgcaggttc tgcagccctg acagactgac ttgttgacat taatcttaat
     2281 tacacccggc ttcataaagc acctcctaaa tattttatcc cgctccgcca acagggaaaa
     2341 acaaaagttc atcaccatcg ttcaagacac agcttttgcc gcggagtctg ccgttgacgc
     2401 tgctctttgt ccattcgccg cttacgggaa gatttatgat attcaatgct tcagcaatgg
     2461 ttgttccttc aggcatttca atttcccgtt ctctaaatcc tgctgatgaa gctaaaatgc
     2521 ctttcagttt aactctgatt ttcaccttgg cctccttcgg gttatttaga ataaaaaaac
     2581 cgcataactc atgtaaatgt ttgcataagc tttgcggctc cttttcaaat aggagggcgt
     2641 ttggattgct ctaaacacct ggcgttctca caggcctcat gggtcagatg agaatcggag
     2701 cataattttc ttattctgcc ataattataa aggttatagc aacaataagg tcaagattta
     2761 tttccgattt tttcttatta tttttaatta ttatgtaatt ttaaccggta tctgcaattt
     2821 aaagaacatt cctctctcgg cgtccgtgag attaggcaga tctgttataa cttcgcagat
     2881 ataatcaccg tcaatggtat agtttctttt tgatatttca tccaaaagcc tgtttgcata
     2941 gggtatttcc tgttcaaacc tgttacagta aatgcataga tatgtgccgg ccttgatagg
     3001 cttgataaga ttttcaggta aattttcatc aacaaataca aataagccac tggatatgaa
     3061 atctctgttt attatttgct cataggctac agtgcttcct gcattgcaga aatatgtctg
     3121 cctaagcccg tttctttcca tatcttcctt cagctttctc agctgttcct catatatttc
     3181 tataccgtag tcataaaaat tagtgctcat gttatagctg tatatccagc gcttttcaat
     3241 gtattcaaga gcaattgtac cgtcaggggg agaggattca tatctttcat agttgcggac
     3301 ggctctttga agtgcatttt tttgattttt caatgcttct atttctgcat caatattgcg
     3361 gcttttttct tccaaaaacc tttttataag tttggtgtcg caggatataa gctgtacttc
     3421 tatatccttc agccgcatgc caagggactt caaatactgt atcatgtcaa gccttgcaca
     3481 ttgcttcatg gaatagtatc tgtagccgtt aaatttatca acttcaattg gttttaatat
     3541 tccgattctg tcatagtagc gtagggtttg ctctgaaaca ttatttattt tggacatctg
     3601 tccgatagtc aacctgtcca tgtatgccat gcctcccttc tatttaatta taccaagaat
     3661 tgtgaaaata tcaataaaaa aaacggctct tcctgctgcc aagaacaaaa agaaccgtcc
     3721 ctttatattt ggggacattc ctctaccggc aaagactcgc cctatagcag aggtgtgtcc
     3781 ccataccttt aaattaagtt tctttttacg atttgatgta ctacaaatgt tgccacatgg
     3841 tttgcatagc ttcccgttcc gaatccggca tcgtatccta attcctgggc aagctcgtga
     3901 tttatacggg gacctccgca aacaagtatc attttgtccc tcaatccttc tgcttccatc
     3961 atttcaacaa gttgagtcag gttggctata tggacgtctt tttgagttac cacctgggat
     4021 accagaattg catctgcctt tacttcaaca gcctttgcga taagctcttc atttaacacc
     4081 tggcttccca tgttgatagc ttcaattccc ttgtatcttt caagtccgta atgaccgtca
     4141 aagcccttca tattcatgat ggcatcaatt cctactgtat gggcatcagt tcctgtgcat
     4201 gctcctacaa taactacgtc acgtttgata ttttccttga tatattcttc aacttcttct
     4261 ctttccatga attccatatc taccttgggt acttcaataa cggtatagtc aactgtgtga
     4321 gtgcattttc cgtacattac gaaatatgtg tatccaactc ccaggtcctt ggaaaatacg
     4381 actgcaggat catcaaatcc cattttttct gccagctgct tagctgcttc gtttgcttca
     4441 tcaccgtaag gaacaggcag ggtaaagctc atttgaacca ttccgtcatt taatgtatca
     4501 ccgtatggtt ttactcttgt taagtctacc ttctggatac tttccattat tttacacccc
     4561 ccagcattaa atcaataaac gggttaaagt atttttcatc cttttcaaca acgccggcta
     4621 atcccttgcc gcctgtgaaa cttcttttta ctccgccgaa tttgcctttt tcaatagtgt
     4681 caaatattcc ttctctttga atatctctta aaagctcttc agctttagcc agaacttcct
     4741 gtgccctttt ctggattatt ccgtcttttt tgtattccac ttcattgcct atatcacggg
     4801 cattgttgaa gatatattta gcgttctcaa ttgaaagcat tctgtcctga agatggggtg
     4861 tatgaattgc ttcagtaggc attcccagca gttgaagccc ttgatgggtc catatggata
     4921 taaaattaaa cattgcatct tgcaaatgtc ctctgaatat atttcctgtc atatattttg
     4981 taggaggcat gtatttaagc ggtgcattgg gaaatatttc tcttgccatc tgagcctgcg
     5041 ccagctcgta caagaagccg ttttccagca tcggattcat ttcgaaagcg tggccgagac
     5101 ccatttgctc ctcaggaata cctgccttca atgcaaactg ctcgttaatt aattgtgatg
     5161 ccagtactgt gtgggcttct tcataggcat ctgccgtagt caggtagttg tcttctcctg
     5221 tattgatgat gatgcctgca aagccgttga ttattcttga gaaatattgg tcaaccaagg
     5281 ttctttgcat attgatgtct ctgaaaagaa ttccgtaaag agcatcgtta agcattacat
     5341 caagtctttc cattgcaccc attgcggcta tttcaggcat gcaaagtccc gagcagtagt
     5401 tgcagaggcg gatatatctt cctacttctt ctccgacttc atcaagagct ttcctcataa
     5461 tcctaaagtt ttcctgggtt gcgtatgttc ccccgaagcc ttccgttgta ggtccgtaag
     5521 gaacgtagtc cagcaagctt tgtgccgtgg ttctgataac ggctattata tcagctccct
     5581 gctttgcagc agcctgggcc tgcagaacat cctcgtaaat attgcctgtt gcaacgatta
     5641 catacaaata gggctttctt ccctcaccga ttgtatttaa gtattcatcc cttctggctc
     5701 tgttgtttct aattctctct actgtctgct ttgcagtctc ataaactgca tccataattt
     5761 tgttttcatc agcaattgga agttttgtta tttcaatttc tcccgagctc gttttttctg
     5821 ctatttcctg aggtgttttt cccgtgttaa ccatggcgtt tcctatccag aatgctgctc
     5881 ccttagacag tccgccgcct tctttgatag cgtctatcaa cacattggga aggggtttgt
     5941 ctatttcatc aacgccgtct actcccatga ggcgggcgac agttctttca actgtgactg
     6001 ttgtattttt gtcgatgaaa ttttgtacat cgtctgcaat attttttgct gcagtacggg
     6061 cgctttggat taactgggga ttaagattta atttgctttc catgtttcct ccttctacgg
     6121 ggacgattcc ggagtgtccc cataccttta taagaatctt tctgcatctt taatgatttc
     6181 tccgtctatg cctgccattc ttgcaatttt tagtgcatct ttaggtatat cacttgaata
     6241 tttaacttca ataagcctgt aatccatgta tttttgaacc tgaccaatgt tgtctatttt
     6301 aatcaggtca gcttcatcag gtagcctgag tcccttaacc tgcaggtttt gaatatcctt
     6361 atcatttgca acattgtcat aatgagttgt taatagagtt atgcagtcct tgtttttaag
     6421 ataatttacc actgcctttg ttattgcgta accttccttt ggattagtac ctcccgcaag
     6481 ttcatctatc aatatgagac acctgcggtc ggaatttttt attgcttcct ttaaattgac
     6541 tatttccgcc ccaaaggtag aaagaccctt ttcaattgac tgatcatccc cggcggaaat
     6601 aaatatatgt tcaaaaagac aaagctttgc atagtcgcag ggtacaaaca tgccaaagct
     6661 tgccatagct gcaatttggc ccgtcattct gagggttact gtctttcctc ccatatttgc
     6721 acctgttatg cagacgacat ttttattaag catcaaatcg atgggaacat attcaaggtg
     6781 tttatctttc aatgtttttt caagtttaag atttctgccg cctttaattg taatttccag
     6841 ctcatctgtt attgtcggct ctacggaccg ggttttttgt gcataatttg cttttgcgat
     6901 aagatagtcg atttggccta taatctttgt gttttcctgc aatatatgac tatctttctt
     6961 tattgagcct gatatatctt gccgaatcct atattcttcc tcatcctcat caaaggtcaa
     7021 ttcttcatag aacttctcgt attcgtcaat ttcatcggta ttcttcagct tgtaaataat
     7081 gtttaaatag ttttctccgg atactctcaa gccgccttcc ttgtttagct cttcgattct
     7141 ttccttttgc gacttgctta ttgttatttc atcctttaaa tttaacttta tactgtgttt
     7201 ttcctgtatt tcttctttta tttttttctt taataaactg acagtttttt caatctgtct
     7261 ttttttattt cttatttctt ttaatttttg cgaatattcg tcgtaaatat aaaatgtgct
     7321 taatttttca taagcggggt caagcttttc atataggcgg ggcaggggcg ttaattgtaa
     7381 cagttctata tcattatcag aaatgcctct taatacttta tccaattttt cgactaaaat
     7441 aagaaaattc tttatttcga ataattctac ttcgtcaaga acctggttgc ttcttgccct
     7501 ttggatggtt tcaagtatat ttttaatatg ccttaaaata tcaattattt cccgtctctg
     7561 tctgttttca ataaaagccc ttattttatt gaactcagtc ttaaggtctg cctcctgacc
     7621 ttttatataa ggttttgctt ccagctttgc ctttattccg tattctgtaa tcggtttaat
     7681 ttcattaaaa atataatcga aatttatgtt tctaactgta ttttctgtca taaagctgtt
     7741 caatcttcat caccgcccga caccacatca actatattta ttcccggaag ggtttttttc
     7801 atattttctt ttagaacttc actgtcaaag aaatagcctt ccggggatac gggatttaaa
     7861 gttacggcta aaacatttat tgtgtccaaa gcttcaactt taagtccctt cctcctcatt
     7921 tcattccaaa cgtttatatc cgtaaatatt tttgttccat cttctataat taaattaatg
     7981 cctcttaaat atatatttcc ccataactct ttcaacaagg cagatgtaag ggcaccttta
     8041 ataaatatgt gacttgtttt tccgtcaatc agctcgctga tttttctgcc tccggtaata
     8101 cttgttttaa tatcgggaac aagtatattg ccgtcttctg atatgatgca tgttttattt
     8161 atttttcgaa caatgttttt aacatgcacg tcagttcccg gcagtgagta gcattccaca
     8221 gcatgggcag tcttttccaa aacctttttc atgtctctgc tcagtacagc cccggttgca
     8281 ataacacatg cgtctgtaat aacaggagat gaaacagctt ttctgtctat tgctccatct
     8341 atgaatacca cttctgctcc gtaatgttta aggcgtcccg caacatattt catatccttt
     8401 gcgctgacag gcccggcaat ctgtatattg cctttctgtc tcacccttac tattatcact
     8461 tcgcccatgg cagttgatat tccggtagtc tccaatattt cagtcctggc atttgaaagc
     8521 atcagtgttt ttttggcagt tgctgcatac attccctctg ttaccataat tgcaggtttt
     8581 tttgtttgtg ttaccaggtc tgtattttca ccgtcccgcc ctgttgaagt aatgcctgtt
     8641 ttaatattta aattttctgc ttcttctatg agataattga gtgtcacggt cttccctgca
     8701 ttctttgcaa ggcctattat ggacactgtt tcataattgt tttgttttat ggtttggatt
     8761 aatctcataa aagcccctct taacaaaatt acaatgtaga tgtataaagc gcagtccagc
     8821 agatcttaat gatgaactct gtctcttctt gccagttttg taggttcaag tgccaggtcg
     8881 tttccttgca gcagtcctgc aactcctgtc aaaggatctt ccttcctgcc ctggcataca
     8941 tcgcacacgc attccagttc cggtatatca ggttctgtat atgtggtaat aacaccttcg
     9001 aagtttctca aaatcaattt gtgtgccgac cttgacaata cataatcagg tcctacaggt
     9061 gttttacctc cgccgccggg tgcatcaaca acaaaagttg gtacgcagaa acctgaagta
     9121 tgtcctctca agctttctat tatttcaata ccctttgcca caggtgtcct gaaatgctca
     9181 attccaagtg aaaggtcgca ttgataaata taatatggtc taactctgtt cataacaagt
     9241 ttatgaacca gctctctcat aatatgcgga cagtcattaa ctcctcttaa caatactgac
     9301 tggtttccta aaggaatacc tgcatctgcc agctttctca aagcttcaat tgattccgga
     9361 gtaaattcac tgggatgatt gaagtgagta ttcagccata ttggatggta ttttttaagc
     9421 atgtttacca aatcatctgt aatacgctgt ggtaaaacta caggtgttct ggagcctaaa
     9481 cgtactattt ccacatgggg tatttctctt aattttctga tgatgtattc taatttgctg
     9541 tcagaaacca aaaggcagtc tcctcctgat aacaagacgt ccctgacttg cggagttttt
     9601 cttatatatt cgatacattt gtcgatattt tccattccta cttcatcatc gctttgtcct
     9661 gcaaatcttc ttcttgtgca gtgcctgcaa tacattgagc attggtcggt aattaagaac
     9721 aatactctgt caggatagcg gtgagttaat ccgggagccg gtgaatctga gtcttcgtga
     9781 agggggtcaa gcatgtctgc agggcttacg tgtgtttctg caaatgttgg tacagcctgc
     9841 atccttattg gatgtctcgg gtcgtcttta tccatatggg ctgcatagta tggagtgatg
     9901 cccattctga atttttccaa taccttgttg atatcgtctt cttcctgctt tgtaatgtta
     9961 attattttct ttaatttctc tacagagtct attctgtttt caacctgcca tctccagtca
    10021 ttccactctt ccggcttaac gtctttccat agttctattt cattataata agccattttt
    10081 atacctcctt atagtattat aaacaagcgg catatgtcat tcaccaaggt gaatgacata
    10141 ttgcttattt tgacttattt tctattatgc atataatttt gtgaataatt ctcttaatcc
    10201 tttgctttct ctcattactt gaagtgctgt ttcagaatgt cccttggtat aaccgtttcc
    10261 aactatcatg tttatatcct tgcccacgcc ttcagctccc aatgctgctt ttgtaaagct
    10321 tgtagccata ctgaagaagt aaacagttgc ttcgtctttt gcagcaagta ttgctgacat
    10381 ttcgcagtta ggtcttgata cacagctgat taccaggtca cataatcgtc cgtctgtagc
    10441 ttccataact ttctcgtaca cttcaattgc atttgtagca tcgccggata ttaaaacatc
    10501 tgccagtcca agttctttta ccgtattcag cgatgcctgt ctgtgggccc agcatattac
    10561 ttttcccgta gggcctgctt ttttctttgc ttcatataag cannnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnggccaagt gccgaaaatt cagctttttg tattgatgga
    10741 ttgataaatt tgtgatatac tttctttgtt gtatgatata taatttctct attgaaatta
    10801 ttcatgccat gaatcgtttt aattgtagtt cggagggctt tggcctaaaa tggccacaaa
    10861 agccccgtcc cttatgataa ataaaatatg gcaaaataga attaaacaga agggcggcaa
    10921 atataacgcc attatgtaag cgaggttaga aatggataac agacctattg gaatttttga
    10981 ctccggggtt ggaggattga ctgtaatgag ggaagttatg gagcagcttc cctatgaaga
    11041 cataatttat ttaggggata ctgcaagaat tccctacggc tctaaatcag aacagacaat
    11101 aaaaaaattt gcacatcaat gctctgaatt tttaaaaaac aaggatgtaa aaaccatcgt
    11161 tgtagcttgc aatactgcca gctccgtggc acttgaccaa ttaatcaggg actttgatat
    11221 tcctgtcata ggagttatcg agcccggggc aagaagtgcg gctgaagcta ccgaaaccgg
    11281 cagaatcgga gtaataggca caacagccac cataaactcc tatgcctacc agggaaaaat
    11341 catggaatac ttgaataatg cagaagtaat aggcataccc tgcccccttt ttgtacctat
    11401 tgtggaagaa ggctgggaat actctcaagt tgcaatgctt actgcagaaa aatacttgac
    11461 ggaactaata gagcacgata ttgacgcatt ggtattagga tgtactcatt atcccatact
    11521 caggcatatt attaaaaagg tcctggggcc taaagtaaaa ctcgtaaacc ctgccttcga
    11581 aacagctaag gatttaaaaa aactactggc ggaaaaagac cttttcaatg aaaatcatag
    11641 caaaccaaaa tatgaatatt atgccactga tgcaccggaa cggcttagaa gaattggagg
    11701 caatttttta aaaaaggaaa tagatgctct ttatcaagtt gctatagata acctttaaga
    11761 aagggatgta acatggaaga tgtaaaaatc agaataaaca ccacacagac aattgatgag
    11821 gcaggcaatg aagatgttat tgaactgctt acagaagcaa aacttgagaa gttgaaggat
    11881 tgcttcataa taaactatga tgaaagcgaa ataaccgagc aggagggaag ccgtacaaga
    11941 ttaaaggtat ataaggataa aatgttaatg accaaggtcg gagtttattc ttctaaaatg
    12001 gagtttcagc agggaaaaaa atacaaaaac ttatattcta ctccttacgg taattttgac
    12061 ttggaatact atactgtttt atatgataat aaattaaacg aaaaaggcag aggcagaata
    12121 tatactgaat atttgattac ttttgccgat tcggacgaaa gctacaataa gcttaatatt
    12181 gatataatgt agacaaagac gaacattaca gcctgttttt cggcattctg tgcctaattt
    12241 ccggcactgg aggaaagtat ggaaattaaa tattttgacg ctttaataga agcaatagaa
    12301 ttaattaacg atggtattca aatcattgat gccagcggaa aaattgtata ccacaatcct
    12361 tcatcaaaag aattggattt aattgatgcg gaaaaaacaa tcggcagaca catacttgaa
    12421 gtatatcctt ctcttacttt tgaaacaagc actattatga atgtgcttaa aaacggcaag
    12481 cctatctata atgttgaaca aaattttgtc aactataagg gtgataaaat ttctacgtta
    12541 aataccactc ttccaatctt gtacaataat aaggtcatag gggcattgga agtttcacga
    12601 aatatgacaa aggtaagaga attgtcggaa agaattgtaa acttgcagag ggagctttat
    12661 gaagccggtc aaggtgcaga aaaaacatca aagcctttgg caaaatttaa ttttttggat
    12721 ataatcggtc agagcaaaga aatactcaag cttaaatccc ttggcctcaa agctgcgatg
    12781 actgactctc ccgttatggt tgccggaagc acaggaacgg gcaaggaact gtttgtgcag
    12841 tccattcata attccagcga caggaaattc aaacctttta tagcccaaaa ctgtgccgcc
    12901 ctgcctgcca atcttcttga aagcattttg ttcgggtcgg caaagggaag tttcacagga
    12961 gctgaaaaca ggccgggtct gtttgaatta gcagacggcg gcactttgtt cctggatgaa
    13021 ataaattcca tgcccttaga ccttcagacc aagcttttgc gggttatcca ggatggcagg
    13081 gtcagaaggg taggctcaag caggacaaca gatgttgacg tgagaatcat atccgctatt
    13141 aatacaaata taggaacagt ggtggaaaca aaacaaatac gccaggattt attcttcaga
    13201 cttaatgtaa taaccatggt gatacctgat ttaagtaaaa gggttgaaga tatacctctt
    13261 ttggtagacc attttattaa aaagtacaat gaaaaatgca ataagtactt caatggaatt
    13321 tccagggatg ttttggatat atttttagat tacagctggc ccggcaatgt aagggagctt
    13381 gagcatgcca tagaaagcgc catatccttg tatgacggtg aaattataag ggaggagcac
    13441 cttcccttcc aatttaaaaa ctatcagctt aacggcagct cattggacac cggcacaatc
    13501 caacacttga atactgccgt tgaaaaggtt gaaagggagg ttattttatc cgcattggaa
    13561 caaacagaca ataatattac aaaagcagct aaacttatca atgttccaag acaaaccctg
    13621 caatacaaaa tcaaaaaact ggaaattctc ttctgaattt caacgacggt tctttttgtt
    13681 ccctggagta ttctcagaaa actaaaagaa cagtacccaa aatttatttt actaaaccgg
    13741 tacttttgat gaccatgtcc agatgtatgt ttatatctgc cgcagcgaac acctcatcgt
    13801 atttgccgga gattttttca tagtccttct tgtgatattt aaataaacct tccttaattt
    13861 ttaacaaatc tgttctgtaa acattctgaa gcttatccaa agcagattcg gaaaccgtaa
    13921 gaatgctttt tatggcttct tctttaattt tattaagata actttggtct tcaagcttat
    13981 tgcttcctat gataaatgaa tctatgtagc tgttcagcgt agcatagtaa tcaactgtta
    14041 atttatcgcc gcttatattc atatcagttg tcataataat atttacaact cctaatgaaa
    14101 catccgtgcc ttcaaccata atatttacgt ttctgatttt agctttgttg ggattcaata
    14161 caaccataac tgcagctact tcatccggct ctaaaaatcc ctttaattca tattccttaa
    14221 aaactccggc tctttcaatt tttaaacttt tttcgttaac ttcaacaaca ggtatcattg
    14281 tatttcctgt ttttccaaga tttaaaatcg cctcatcaaa tgtatagaca tcctgataat
    14341 tttccctctt aagcttaatg agcatttcgc ttaagaacct tccaacaata ggattgctgg
    14401 gtatttcagt gttaataata tcctgtgcct tgccttttgc aatgcatatt ttaactcttc
    14461 tgttcatttg cggcgaccgc tggatttcat caaagattct tttgactgtt tcagaatccc
    14521 tggccacatc ctccccgagt acaattacct gcatcagcct gtctgaaata gccttttctg
    14581 aatgcaggta gcttgttgca taaaaatcgg caaaattctc agaatccttt gtatatacca
    14641 atctttgttc ttcacttcct tcatttattt ttggtatctc tgaagtgaag gtaagatttt
    14701 tatcattttt atcaattcca actgcgaata tataatccct tttgtttatt tcaatactgt
    14761 cccagcagcc ggtcaacaag atggatgata ttacagttat aattataatt cttatatttt
    14821 tcatttatac ctctatattt ttttagttct tccagcggtg tttagttgtt atgaattgct
    14881 gtaacgtcag tatcatgctg aatgaggata gggtcaggca tcttccgatg ccgctcaagg
    14941 aacaattcaa tcatttataa ttcaggatca taagcactat ttattttttg cttttttaga
    15001 cctgacgtcc actaaaacag ctgttaaggg tattaataaa ttcaagctgc taaatacgac
    15061 tatgggccat tgaatgtatg gaaacatcat gtacattccg ggtaatacaa ctgccataat
    15121 atataagaca gggatttgaa taaatacaaa gtacccgttt tctcttgtct taaaggtctc
    15181 ctgcatcgcc atatttgtaa aatatgcaat aatgcatata catgaaaatg taactattat
    15241 ctgaaagcac aaaccaacaa tttcagtgct ttcaaaaaaa aatccgggaa tatttacata
    15301 cttcataaca gaaataaagg gccataccaa atgtactgat tccttaatcc caaacttgac
    15361 aattataagc aaatagcaag caatatacaa aattgaactt attaggataa actttctgct
    15421 tataaatctg ttgttttctt gaacagtgac catgctgttt gaaaacaata atattgaaaa
    15481 accgaagaat cccagtaccg acccggatac agaccttaca accccgttaa tgtcaaaggg
    15541 gtatatagga aaaacatttg tataatcggc ataataggta gaaaatatta ttatgagcaa
    15601 atatggtata aaagctgtta aaagggctat ggatgcaata tttgcaattg tttttattcc
    15661 cttctttgtg gcataacttg cggtaagcaa aattacaatt ataattgttc ccaggggagt
    15721 tctgaaaagc atcatcaact ttatttgttc ggcaaaatct ttcattaata tgctgtttgc
    15781 agttaaaaac attaatatat agtatattcc tgtcaatctt gataaaaact tgggaaatat
    15841 tttatttatt atacttaata cggaatcatc cttatatttc agcatcattt tattaattgg
    15901 ctttaaagca aaatataata caaaagctgc aattattata gtcatccagc ccacaggccc
    15961 gtcaatttca gataaaagaa gtggcatcaa aatagattga aaggcaaatg atactaatat
    16021 taaaattgat agattttgat acggcgtaat tttctgttca aacattattt tccattttcc
    16081 ttttctacct gcaaatattt tggttttcgc acaagagttt taatttttgg tcttattata
    16141 gtatctttca ggtcgcttct tctttctaca aaatgagtca taggtgttaa ataaggcacc
    16201 ccaaaacttt tcaatgtaca tagatgtata attaaaatac acagtccaat tgatattcca
    16261 aataatccga aaaaggaagc tgatattata aaaccaaatc ttatcattct tagtgatgtg
    16321 ctgaaattgt aacttggtat tgcaaaagaa gccagggctg tcaatgcaac tattattaca
    16381 gctattggcg ttattaaacc cgcctcaacg gaagcggagc cgataaccaa accgctgact
    16441 aaacctatgg tggaacccac cggagttgta attcttagag aagcctccct cacaagctcc
    16501 attaccaatt ccattaacaa tgcctcaaaa tatggcggaa agggtacgtt ggctcttgaa
    16561 gcagccacat acagcgcaag ctgtgttggc agcatattag gatgaaattg tgttacagct
    16621 acataaatcg caggcaaaag tataactatg ggcagtgcta tataccttat catccttatt
    16681 aaacaagcca gcatccatcg ttcataataa tcctccgatg actgcataaa tgatacgaag
    16741 gttgcaggcg ccatcaatac agtaggtgtg ttgtctactg caattacaac ccttccttcc
    16801 aacaacgccg aagctgccgc atcaggcctt tctgtgtttt caacctgggg aaagggtgaa
    16861 taaacatcat cctcaattaa ttcctcaatg taggagcttt ctaaaactgc ctctatatca
    16921 atgtttgtaa ttctcttttc aacttcctcc aagactgttt tatttaccag atcctctata
    16981 tacattatag caaggtcagt cttggatctt gttcccgcct gcatgtattt tacttttagc
    17041 cttgggtctc ttattcttct tcttataagg gtgatgttga ttttcattgt ttcaataaaa
    17101 ccatcccttg gtccccttat aagagtttct gctgcaggtt cctgaatacc tcttaattgc
    17161 catccccttg atgaaagcca tattgccctg gaacatgtat ttataaacaa aattgtttcc
    17221 ccggacaatg ctttgtcaac acattcctgc aatgtattca aaactccaag ttcggttatg
    17281 gcaatattag tttgcaaaac cttgttttcc agctctattc cgttactttt ttctaaactt
    17341 tccaaatcaa tagattccat taaagtttga atagcaaatt cgcttatgga atcctttgta
    17401 gtcatcccat caacataaaa aaggagtatt tttagctttt cattttgtcc taccttcatg
    17461 ttgcgaaata ctatatcatt ggcatcccta aatgtttctt ttaaaacatt ttcattcttt
    17521 tttaaatccc tatcaacctt tatgttagct aaatcctgat aaatactcat aatatctcct
    17581 aaatattttt tgctataatg gctcctattg caattaatcc gtaaatgatt cctgtaatcc
    17641 tgacaatttt ataatctctg tgatagttct tgcgtttaaa gtccttactg actttgaata
    17701 ttaatatata gcttataatt agaaaaacta taatgacatc gatgtttata accggtttta
    17761 tatgatttat taaagacata cttcctcctt ttcatgaatt atccaggcaa tagtaatatt
    17821 tacataaacc gctttatata taccaataat tttcagaata acaagaaagt ggacacacct
    17881 ataagtaagg agtacgtaca ttgctgccat ttgtttgccc ctatcccttt atgtttgata
    17941 aaattaataa aaaaaaagtt aaggaacttg tgatcttcct taactctatt attttacatc
    18001 tccggttgta ctactattta gaaagagata cggcataatc aggtgagccg gcaaaagtat
    18061 atatgccggt tttttcaact cttccttttt caatgaattc aaatattttt gattttaact
    18121 ttatattctc tattcctctt tttgttctaa tggtatcaat ttcatccaag gttaatactt
    18181 cctgaagcct tttttctgtt tcaatatcaa atactgcagc atctcgggtt tttccgaaat
    18241 ctacgaagca gaggggcgga aacataacgc accaccagtt gctgccttct gcttttccta
    18301 tttcaattct gaccgcatca taatacccct cagggagtac tatttcttca tacatctttc
    18361 gaggaaactt ataatttcca taatatacat taatttcata gttataacct tcctctttta
    18421 ctgtttcttc tgcttcacat cttatttgat gaatcttttc taaaacaata ttttcgcttt
    18481 cttccttgga atcaattttt tcaaattctt cattgaatgt tgcaattatc cggttcctaa
    18541 ccttatattt aagctgctgg tcttcaaggg tgtcagaatt cgcaacaaca tgaagacgta
    18601 ttacctttcc gttcaatctg gatattcctt catatgcttc aactgtatat gcaattccta
    18661 tagctaaaat gacaaatatt gctgaaataa atattttctt gtaataagac attttacctc
    18721 ctgatttatt taatatttta attaatggga taagctgtta gtaatatctg catttttcag
    18781 gtttttatac taaaataatg aaataacctt agaaatacaa gaaaaaaggc tcttttaagt
    18841 atgaaagaac cttcttatgc tgacagcgct cccgtcccct tgtcatctac agactgtatt
    18901 tctgcttttt tcttaccaga gttgatatgt caatgcccag cctcattgct gcttctttca
    18961 tgttttttga aacagccagt gttgattcaa taatttcttt ttcatattct tctgtttttt
    19021 cttttaatgt tttcgtacct gttacaaaaa gctcagcggg gtctgtgccg cccggcagta
    19081 tcaaatgctc cctaaacaaa tctgttccga tttctgctcc gtctgccatt aataccaggc
    19141 gctcgattat atttttcaat tccctgatat ttcccggcca ctcatatatt aaaaaactct
    19201 gaagacagct tgaagaaata tagcagtttt ttccgtactt gttattgtaa aactcaagaa
    19261 actgggtaga caaggggata atgtcttctt tcctgcttct caggggggga attacaaatg
    19321 taactacatt taaacggtag tacaagtctg ccctgaattt cttttcttcc acaagctcct
    19381 tcaaatcatg atttgttgca gctataatcc gggcattaaa ttcaatgggg ctggtgcctc
    19441 caatgcggta gaacatttta tcatttattg cacttaagag tttgacctgg agggtataag
    19501 gcatgtctcc tatttcatcc aggtagagtg ttcctccgtt agcttcttca aaaagacctt
    19561 tttttcctcc ttttacagca ccggtgaaag caccttcttc gtagccgaaa aactctgatt
    19621 ccagcagatg ctccggtact gcaccgcagt ttatttttat gaatttaccc ttgtttctgc
    19681 tgctgattga gtggatatat gttgcaataa aatcctttcc cactcctgtt tcccctaaaa
    19741 ttaaaacagt gctgtcaacc ttggaaattt tttttgcttt ttcgtatata tttttcattt
    19801 taatattatt tataatttct tcagggtcat gggaagactc tgtttttaca ttgtaatcgc
    19861 tttgaatgaa caggtttttt tcctttgtaa tagtcttcat caacacatcc agttcagaca
    19921 catctcttat gactgctatt gtttttgaca ggtttccctt ttcatcaaaa ataggcgagc
    19981 ctgaaaccag acacctctgt cctttgtagt agtggtttat ggttgatacc ggcccctttg
    20041 tttcaataac cttggcacag catgaattag gcagtatatc acttttcctc aaatcataaa
    20101 ccgtatttcc gataatttgt tctcttgtaa gccctgacaa gtttaagaat gcatcattga
    20161 catatatggt tgtaccaagt tcatcggtaa tatatattcc gtcttttaat ttgttcagca
    20221 tttcattata tagatattgc tgggagttga gtctttcgat ttgtttttgc agctcgcttc
    20281 ctattgtaaa atcataaaag aaaaaactgt ataattcatg gttgtatagt ttaaaattat
    20341 tatggataac ataatattcg gaattatcaa tgtacctgac agaataaaga tttttatcca
    20401 tatcaacatt aatattctga aaaaacattg ttattttttt atttagtatg tcagtcttga
    20461 ttatatttgt tttaaaatat tcattgcagt ataaaacatt aaatttatcg tcagttataa
    20521 taaagcccag gtttaaatca tttttcatca atggcatttt tatctccttt ttatatatct
    20581 atataaa
//
LOCUS       DAOPUT010000019        19758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_2775_length_19758_cov_18.821093, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000019 DAOPUT010000000
VERSION     DAOPUT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 19758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19758
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_19758_cov_18.821093"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PKK75_03285"
     CDS             complement(<1..775)
                     /locus_tag="PKK75_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNZ82649.1"
                     /translation="MSQGRVVKISGPLVIADGMKEADMFDVVRVSEQGLIGEIIEMRG
                     EMASIQVYEETSGLAPGAPVVSTGAQLSVELGPGLIETIYDGIQRPLEEIRRVAGPNI
                     TRGISIPALNREKKWDFVPAVKAGDRVEAGDIIGTVQETIVVEHRIMVPFGVSGEVAE
                     IKEGSFTVEETVCVVKKDSGEHVNLTMMQKWPVRKGRPYKEKLVPVSPLLTGQRVIDT
                     LFPISKGGVAAVPGPFGSGKTVVQHQLAKWADAEIVVYIG"
     gene            complement(808..1128)
                     /locus_tag="PKK75_03290"
     CDS             complement(808..1128)
                     /locus_tag="PKK75_03290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNZ82650.1"
                     /translation="MYKIGIIGDKQSVQGFKAVGLDVFDCSRADEARRTLHKIADDNY
                     AIIYVTESLYNEIKESIYEYNQERLPAIIPIPGMQGSLGIGINNIKKAVEKAVGADIL
                     FSND"
     gene            complement(1121..2128)
                     /locus_tag="PKK75_03295"
     CDS             complement(1121..2128)
                     /locus_tag="PKK75_03295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HNZ82651.1"
                     /translation="MAKIKENDYLYSTSRVKSIEKHMLTRERAEKMIDAKTMEDALRV
                     LDDCYYGYSNEVTDFNDYEGLLAEEHKKTYDFIRSIAPEAEHFNLFLYPYDYHNLKVL
                     MKNEYLNKNETDILVDTGTIDLKVLMHALKERSFTDLTENMGKALNEVIEEFPKTKDP
                     QLIDIIFDRYCFDEMLKAAEKTNNEYIIDYVKMQIDSNNIKTFVRLKKMKKSWDFFSR
                     VFLQGGKVHEHIFIRNYDEPFEKFGELLLAYDFKEVFLEGTEALAETGKFTKLEKLLD
                     NKLMEHVKSAKYFPYGIEALAGYLIAKDNEIKTARIILAGKTAGISPELIKERLRETY
                     V"
     gene            complement(2156..2737)
                     /locus_tag="PKK75_03300"
     CDS             complement(2156..2737)
                     /locus_tag="PKK75_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HNZ82652.1"
                     /translation="MSIENITSKILSDAQNNADSSLKSAESTKQELLNKAKAEAEALI
                     KTEAEKAANDAVELKNRRVSAAELQGRKMLLSAKQEAIKKSFGAAIEKLKAMPEDEYL
                     SFLGQEIIKIPNCEGTIVLNAKDRENIGERLVKAVNEKLGAEKVVLSKNTVKASGGFV
                     LKKGSVEINNTFETLLDSMKDELTGSIANALFK"
     gene            complement(2775..3251)
                     /locus_tag="PKK75_03305"
     CDS             complement(2775..3251)
                     /locus_tag="PKK75_03305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNZ82653.1"
                     /translation="MEFTMGGFLAILGAANASMAGIGSAWGVAIAGMAAGGVIAEDPK
                     KFGPTLMLQALPGTQGIYGLLIAFIIMNKIGVLGGSIAPLTTAQGLMFLAASLPAGIT
                     GTISAVWQGKVAATGVQLVGKRPEEIAKAMVYAAMVETYAVLALLASFLMVNGIQV"
     gene            complement(3254..5236)
                     /locus_tag="PKK75_03310"
     CDS             complement(3254..5236)
                     /locus_tag="PKK75_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HNZ82654.1"
                     /translation="MTIVNMNKINIVGLDSIKTELIKRIMDLGVVEISSQDFKLTDPE
                     WNSYVKKDGNENEVFNLDVKISQVSEVLNSLEKYDTSKRPLFNTRKPVSSAEFIKALE
                     KNDSVSENVNRVLGLNKSLSELCSEGNKIEAAILSLKPWIKYPIPLDMTETKYTSVII
                     GAVPSIADINELKSKLFRTTERCYMDVVDSDKDQYYCSFICLTEEKEDILELLKQYGF
                     SPVTFKDLRGTAEENIMLYENSLKEISAKKETVDKDITELVSFKEEIQLYHDHLVIER
                     DKNKVLSSMLKTDTTFYVEGWVPEACKDSVQKVLDQYQCWYDFKKPEEGENFPILLKN
                     NAFAEPFESITELYSLPATSNIDPTAVMAPFYVLFFGMMLGDVGYGVMFALGCYIILK
                     KFDIEGTFGRMLKVFFWGGISTIFWGVMYGSWFGDGLVVGARHLFNINLPITYVWVDP
                     LKEPMVVLIVSFVMGLVHLFVGMGMSAYMSIRDGHLADALMDVGLWYLFILGPIFWLA
                     GDMIIPGSVLPVIGKWVTIASAIGLVLTQGRAKVGIINKLISGVLALYGITGYLGDVL
                     SYSRLLALGLATGVISSVLSLIGSMGSGITGAIMYVLVFVFGHTLNFAINGLGSFVHS
                     ARLQYVEFFGKFYEGGGDAFAPFTKKTKYIKIKEEI"
     gene            complement(5223..5549)
                     /locus_tag="PKK75_03315"
     CDS             complement(5223..5549)
                     /locus_tag="PKK75_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82655.1"
                     /translation="MSIEVIKVIKEAEEKAEAIKKDAAAQARQVITNANAKAQEILDE
                     ARKKAESDRSEVLSKAESEGQLLYDGIINDAARDCDEILTKSGQNMDKAASIILERIV
                     KTSDNS"
     gene            complement(5934..6359)
                     /gene="fabZ"
                     /locus_tag="PKK75_03320"
     CDS             complement(5934..6359)
                     /gene="fabZ"
                     /locus_tag="PKK75_03320"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNZ82656.1"
                     /translation="MNYDINDIQNIIPHRYPMLLIDKIIELEEGKMAVGIKNLTMNEQ
                     FFQGHFPGNPVMPGVLIIEALAQTGAFAILSLEKFKSKTVYFGGIQSAKFKRKVLPGD
                     TLKLVTEITKLKSTFGIGSAIAYVNGEIAAEATLIFAVE"
     gene            complement(6364..7599)
                     /gene="fabF"
                     /locus_tag="PKK75_03325"
     CDS             complement(6364..7599)
                     /gene="fabF"
                     /locus_tag="PKK75_03325"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNZ82657.1"
                     /translation="MKRKVVITGIGVVSPIGTGDIFWENVKKGKCGINFIESFDTSDF
                     SVKLAAEVKDFDPVLYMEKKESKRMDRYSQFALAAADIAVKDSGLDTEGIDSDRFGCI
                     VGSAVGGIHTIEEEHQKLLTKGPGKVSTFFIPMMLSNMASGLIAVKYQAKAVNISTVT
                     ACATGANAIGESYKRIQDGICDVMIAGGAEAAITPCSIAGFTVLTTLSTTTDINRASI
                     PFDKDRHGFVMGEGAGLMVLEDYEHALKRGARIYGEIAGYGATCDAYHMTSPDPSSEG
                     ATKSMINAILDAGIQPEDITYINAHGTSTLYNDKFETQAIKNVFKEDSKNIYVSSTKS
                     MTGHLLGAAGAVEAIICVKAIEDSFIPATINHAETDPELGLNFVPNVGISKEFDYALS
                     NAFGFGGHNATLVLKKYKG"
     gene            complement(7781..8512)
                     /gene="fabG"
                     /locus_tag="PKK75_03330"
     CDS             complement(7781..8512)
                     /gene="fabG"
                     /locus_tag="PKK75_03330"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNZ82658.1"
                     /translation="MKTAIITGASKGIGAVLAKRLNELNYNLVLNYRSSTASMEKLIE
                     NFTNRETKNIIVKCDISVYEDAKILIDEAYNNFCTVDVLINNAGITKDNILPLMTEEE
                     FDQVIDTNLKGTFNCCKHIARRMIKQKQGRIINISSVSGLAGNPGQVNYSASKAGVIG
                     MTKSIARELGKKNILVNAVAPGFIKTEMTDKIPDEIKAEMMKNIPLQRLGQPEDVADC
                     VEFLISDKASYITGQVISVNGGFYM"
     gene            complement(8530..9462)
                     /gene="fabD"
                     /locus_tag="PKK75_03335"
     CDS             complement(8530..9462)
                     /gene="fabD"
                     /locus_tag="PKK75_03335"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNZ82659.1"
                     /translation="MNKTAFVFPGQGAQFAGMGKDFYDNFKESREIFHRANEALGFDI
                     TKLCFEGPDEELSVTKITQPALLTVCLAIYEVLKKNDNSAVMGGLSLGEYSALTAAGA
                     MDFETAVRLVYKRGNYMQNTVPLGEGGMLALLGCTDEDAIAFCNDITGKYGLLEPANF
                     NCPGQIVTGGRSTAIDNAVDKAPGFNIKRAIKLQVSAPFHTSMLKPAGDRLKEDLSKI
                     KFKRPVRDVIANVDTLYYEIGKIAEKLEKQVYNPVRWEACVRKMIGDGVNTFVEIGPG
                     KTLTGFIKKIDKNVKSINVDSVQGLNSYLQFAGA"
     gene            complement(9455..10411)
                     /gene="fabK"
                     /locus_tag="PKK75_03340"
     CDS             complement(9455..10411)
                     /gene="fabK"
                     /locus_tag="PKK75_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HNZ82660.1"
                     /translation="MIYTKVCELLNIKYPIFQGGMAQISDAGLAAAVSEAGGLGIIAS
                     GNNPPESIKAEIHKIREMTDKPFGVNVMLLSPYSQEVSELLIEEKVPVIITGAGNPGK
                     YMKVWKERGIKVIPVVPSVAFARYFEKAGADAVICEGTEAGGHVGEITTMCITPQVVD
                     AVSIPVIAAGGIGDGRGIAAAFCLGAQGVQVGTRFVLAKECRVHQNYKDKIKKASDTD
                     TAVTGRKTGHPVRVLKNRLVTQFKDLEKNNAAVEEMEELGRGKLYKAAVEGDVDYGSV
                     MSGQIAGLVKKEQTCMEIISEMFEEAEKVMKNLIVSQGGRNE"
     gene            complement(10450..10683)
                     /gene="acpP"
                     /locus_tag="PKK75_03345"
     CDS             complement(10450..10683)
                     /gene="acpP"
                     /locus_tag="PKK75_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNZ82661.1"
                     /translation="MILEKVKEILVEELDVEEESITLESKIKDDLGADSLDLFELINK
                     IEDELDVVIEEEDYGKLITVGDIVNYLTERKQI"
     gene            complement(10806..11924)
                     /gene="wecB"
                     /locus_tag="PKK75_03350"
     CDS             complement(10806..11924)
                     /gene="wecB"
                     /locus_tag="PKK75_03350"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNZ82662.1"
                     /translation="MDKIKVLVVFGTRPEAVKMAPVVKALKKDKDTFESKICVTAQHR
                     DMLDQVLRIFDIEPDYDLDIFERGQTLTQITCRALTGLEEVIEDFKPGLILVQGDTTT
                     VFSGALAAFYHQVKIGHVEAGLRSGNLYSPYPEEANRMMTGVLTDFHFAPTETSRDNL
                     LKEGYDENKIFITGNTSIDALSWVIDENYKFEEEIINNIDFKNKKVILLTAHRRENIG
                     KPMENIFSALKDIAEKNKDVEIIYPMHLNPKVREIADKIFKGMESVHLIEPLDYLPFT
                     NLMNKCYLVVTDSGGVQEEAPSLGKPVLVVRRETERPEGIQAGTAKLAGTDRDNIFKE
                     LNILLNNKDEYKKMANAVNPYGDGKAADRIVEAIKTNW"
     gene            complement(12114..13145)
                     /locus_tag="PKK75_03355"
     CDS             complement(12114..13145)
                     /locus_tag="PKK75_03355"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNZ82663.1"
                     /translation="MMTANLIAFISAVIISFIMTPWARRLAIKVGALDFPKEARKIHN
                     KPMPYFGGLAIYVSIMACMFVYMPHNSTNYHIMAGATVIMLTGVVDDMYGISAKVKLL
                     IQIIAALIALGGGVQIHFITNPLSQTGISYLFDWLSFFISLVWIVGITNTINLIDGLD
                     GLASGVASIAATTLLFTAARLGYDFIVMQCAIIAGASLGFLPFNFNPAKIFMGDTGSL
                     LLGYMLAVTSVLGMVKSVAVIALAVPVFALGVPILDTAFAIIRRYINKKPIMEADKDH
                     LHHKLMGVGLNQRQTVLIIYFISMLLGIVSVIIADADPFTGLAVATAVVIIAFLFAKK
                     AGLFVRKEH"
     gene            complement(13142..13597)
                     /gene="rpiB"
                     /locus_tag="PKK75_03360"
     CDS             complement(13142..13597)
                     /gene="rpiB"
                     /locus_tag="PKK75_03360"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNZ82664.1"
                     /translation="MKIVLASDHGGFELKEAIKKHLIKKGYDINDIGVNDTKSVDYPD
                     YGKRAAMMVAGKEADRGIIICGTGIGISIAANKVKGIRCALCTNEYMARMSRMHNNAN
                     MLAIGGRVTGVGLAEDMVDVWLSTEFEGGRHEIRVNKIMDMEISKEEEK"
     gene            complement(13594..14082)
                     /locus_tag="PKK75_03365"
     CDS             complement(13594..14082)
                     /locus_tag="PKK75_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HNZ82665.1"
                     /translation="MNILFVCTGNTCRSPMAEGIFKDMLRKNNIDNIGVSSAGLSVFP
                     GDRANGKAIRALEEKGIDISLHRAKKLSDEINGADLILTMTLSHKPGIEDYFKRRTDE
                     SPPRIFTLKEFAAKLSGEKSTGTDVDDPFGKDYSFYKKSRDEIEKELGKILKNIDKLE
                     EI"
     gene            complement(14084..15142)
                     /locus_tag="PKK75_03370"
     CDS             complement(14084..15142)
                     /locus_tag="PKK75_03370"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNZ82666.1"
                     /translation="MENGIITKIVKVDPENIDYNIIKEAADIINRGGLVVFPTETVYG
                     IGADALNDAAVDKIFKAKGRPQDNPLIVHIAELDELYCLASEVPKKAELLAERFWPGP
                     LTMILYKRKILSDKITAGLNTAAIRLPVNKIALALIRESKKPIAAPSANTSGSPSPTE
                     ASHVIEDLMGKVDMIIDGGSTYIGLESTVVDMTGDIPMILRPGGVTLEDIISVLGECT
                     YDPSIIKSEEAIIPKSPGQKYRHYSPKAKVILYKGHIEKVAEKINEDYDKLTLKGLKA
                     GIMSTVQTRELYKGKVNICIGDRTKLLTISSNLFKGLRDFDHMGIDVILAEAVDEKGL
                     GKAIMNRLGKSAGEIIEV"
     gene            15415..16152
                     /locus_tag="PKK75_03375"
     CDS             15415..16152
                     /locus_tag="PKK75_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNZ82667.1"
                     /translation="MNILDYIIMGFVFGGIGTSIGGLISVLIYKPSDKMVSGILSFAA
                     GIMLAVTSFDLMPEAYEIGGFFIVTLGLFIGLIIVFFTKDMIPADKLKQYKGKKLSYI
                     KMSLIIIISISLHNFPEGVAVGSSYVYSNDLGVKIGILIAAHDIPEGISVGVPLVMAG
                     VAPLMVIIITLLTGLPTALGAVFGALAGGISEYFIAFCLSAAASSMLYVSANELIPEA
                     YLLYNGKSSSIMLLLGFLCGCYISFGI"
     gene            complement(16245..16466)
                     /locus_tag="PKK75_03380"
     CDS             complement(16245..16466)
                     /locus_tag="PKK75_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNZ82668.1"
                     /translation="MKYNYMTFKSVSFAMKAESILKKNNLEYKTIPVPRSISSDCGIS
                     IRFRKEDNEVIDSLLKGVYEKIYLDLEIK"
     gene            complement(16476..17060)
                     /gene="yfbR"
                     /locus_tag="PKK75_03385"
     CDS             complement(16476..17060)
                     /gene="yfbR"
                     /locus_tag="PKK75_03385"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HNZ82669.1"
                     /translation="MKKSNFFAFISRMKYIDRWGLMRNINKENVSEHSLDVAVIAHAL
                     AVIQKKRLNMEVNPERTALYAIYHDVSEIFTGDMPTPVKYYNSVIKSAYKDVELSVNE
                     RLLNMLPGDFKEEFEDVLVPKEEEKEIWKTIKAADKISAYIKCLEEEKSGNKEFLKAK
                     QTLLSAIKSMNMPEVEIFMDEFLEGYSLTLDEME"
     gene            complement(17302..19527)
                     /locus_tag="PKK75_03390"
     CDS             complement(17302..19527)
                     /locus_tag="PKK75_03390"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNZ82670.1"
                     /translation="MKKTYKIILLTLILVLCFTFTAFAQDIKVYVDNEKVDFDVQPFI
                     ENGRTLIPLRGVFEKLGAKVDWNKDLLEVVIKDDNNEIEMILGKDKVMVNGIIKDIDV
                     PTRMINSRTFAPLRFAAENLGHTVRWDGNTSSVYITKNDTFPVDKGISTVGTKENLIA
                     LLEYNSKLHNYIWMDRAWVGGIAEDSAAEAPAVQETQAENSGTSNMDKSDTNVQVEGV
                     QEGDIIKNDGRYIYVITDRGLKIIDSNPKAPKVVSTIFVPENTSISEIYTAGNKLIVI
                     GQNNFYYIMEKPAADSPDLTKPLIMPPRYDENRTDVLIYDIENIEKPVLEREYLFDGN
                     YLSGRVIDDSFYLVNNKYLNLGYRYYDNIDIDIPLPYYTDVLRGEKHEFGYDQIKYFP
                     NYIDTRYMYTIGIDLSDKVSKPDVDVYLGGTDTIYVSKDNLYAAVTDYSYDYEIPQME
                     EFNPEYTTSTVIYKFKLDGGNIGTAVQGQVPGTIINQFSMDEHNNTFRIATTTGEMWM
                     NTSKNNVYILDENLKALGKLEGLAEGERIYSTRFAGDRVYMVTFRQVDPLFVIDTSNP
                     RAPELKGMLKIPGYSTYLHMVDENHILGFGYDTEENQWGGTVNGGLKISMFDVSNVNR
                     PKETFTEVIGKAGTYSELLYNHKALMFSLNKGLMAFPVSRMAENYKSDFNGAYVYNIS
                     NDSIKLKSTISHNESEFASYLDNIKRIIYIGDYLYTFSENEMQVHSIDTNKKVNELLI
                     N"
BASE COUNT         5386 a         4476 c         3122 g         6774 t
ORIGIN      
        1 agcctatata tacaactatt tcagcatccg cccattttgc cagctggtgc tgcacaaccg
       61 tctttccgct tccgaaaggt ccgggaactg ctgcaacgcc gccctttgaa atggggaata
      121 atgtgtctat aactctttga cctgttaaca agggacttac tggaaccagt ttttccttat
      181 atggacggcc ctttcttaca ggccattttt gcatcatggt taaattaaca tgttcaccgc
      241 tgtctttttt aacaacgcat acagtttctt caaccgtgaa gcttccttct tttatttcag
      301 caacttctcc ggaaactccg aaaggaacca tgattctatg ctcaacaact atagtttcct
      361 gaacagtacc tattatatct cctgcttcca ccctgtctcc agcttttacg gcaggtacaa
      421 aatcccattt tttctccctg ttaagagcag gaatgcttat tcctcttgta atattaggtc
      481 ctgctactct tcttatttcc tctaagggac gttgtattcc atcatatatt gtttcaataa
      541 gtccgggtcc cagttcaaca gaaagctgcg ctcccgtgga aacaaccggt gctcccggag
      601 ctaagcctga tgtctcttca taaacctgta tggatgccat ttcaccgcgc atctcgataa
      661 tttctcctat gaggccttgc tcgcttacac ggacaacgtc aaacatatcg gcttctttca
      721 taccatctgc tataaccaac ggaccggata ttttaactac tctaccttgg ctcattaaac
      781 taatcacctg ctttcatttt ttttacgtta atcgttacta aacaatatgt ctgcaccaac
      841 tgctttctca actgccttct taatgttatt aatccctatg cctaaggatc cctgcattcc
      901 cggtatagga ataattgccg gcagacgttc ttgattgtac tcgtatattg attcttttat
      961 ttcattatac aggctttctg tcacataaat aattgcataa ttatcatcag caattttatg
     1021 cagcgtacgc cttgcttcat cagccctgct gcagtcaaat acatccaatc ccacagcttt
     1081 gaatccttga acgctttgct tgtctccaat aatccctatc ttatacataa gtctctctca
     1141 acctctcttt tataagttcc ggcgagatgc ctgctgtttt acctgccaaa attattcgtg
     1201 cagttttaat ttcattatcc ttagctatca gatatcctgc aagagcttct atgccgtaag
     1261 gaaaatattt ggcagacttt acatgctcca tgagcttgtt atccagaagc ttttccagct
     1321 ttgtaaactt tcctgtttca gctaacgctt cagttccttc aagaaatact tccttgaaat
     1381 cataagccag gagaagctct ccgaattttt caaaaggctc atcgtagttc ctgataaata
     1441 tatgctcatg aactttaccg ccctgtaaga ataccctgct gaaaaaatcc caggattttt
     1501 tcattttctt aagtcttaca aatgttttaa tattatttga atctatctgc atttttacat
     1561 aatcaataat atattcatta ttggtttttt ctgcagcctt aagcatttca tcaaaacaat
     1621 accggtcaaa aataatatct atgagctgag gatcctttgt ttttggaaat tcttcaatta
     1681 cttcattcaa tgcttttccc atgttctccg tcaaatcagt aaaacttctc tctttcagag
     1741 catgcattaa aaccttaaga tcaatggttc ccgtatcaac aagaatatct gtttcattct
     1801 tgttcaaata ctcgtttttc atcaatactt tcaagttatg ataatcatat ggatacaaaa
     1861 ataaattgaa gtgttcagct tcaggagcta tagatctaat aaaatcatat gtcttcttat
     1921 gctcttcagc aagaagacct tcgtaatcat taaaatcagt tacttcattt gaataaccgt
     1981 aataacaatc atccagtact cttaaggcat cttccattgt ttttgcatct atcatttttt
     2041 ctgctctttc acgggtaagc atatgttttt ctatgctttt tactcttgaa gtagaatata
     2101 aataatcatt ttccttaatc ttcgccaaag tcattcctcc tttcaggcag gaaaattatt
     2161 taaaaagcgc atttgctata ctgccggtaa gttcgtcctt cattgaatcc aaaagggttt
     2221 caaaagtgtt gtttatttcc acgcttccct ttttaagaac gaatccgccg cttgccttaa
     2281 ctgtattttt gctcaataca actttttctg cccctagttt ttcatttaca gcttttacta
     2341 atctttcacc gatattttcc ctgtctttgg catttaaaac tattgtccct tcacaattgg
     2401 gaatttttat aatctcctgg cctaaaaaac tcaaatattc atcttccggc attgctttaa
     2461 gcttttcgat tgcagcacca aaactctttt taatcgcttc ctgctttgct gacaaaagca
     2521 tctttctgcc ttgaagttcc gcagcagata ctcttctgtt ttttaattca accgcatcat
     2581 ttgctgcttt ttccgcctca gtctttatta atgcttcagc ttcagcttta gctttattta
     2641 ataattcctg ctttgtactt tccgcactct tcaaagagga atcagcatta ttttgtgcat
     2701 cgcttaaaat ctttgaagta atattttcaa tgctcatttg atcacatcct tattataaat
     2761 tttaacacac tcctttaaac ctgaattcca tttaccataa ggaatgatgc aagaagtgcc
     2821 agaactgcat atgtttcaac catagctgcg tataccattg cttttgctat ttcttcaggt
     2881 ctctttccaa ccagctgaac accggtagct gcaaccttgc cctgccaaac agctgatatg
     2941 gttcctgtaa taccggcagg aagtgatgct gccaaaaaca ttaatccctg agcagttgtc
     3001 aaaggagcta tgctgcctcc aagtacacct attttattca ttataataaa tgctataaga
     3061 agaccataaa taccctgtgt tccaggcagt gcctgcagca tcaatgtggg accgaacttc
     3121 tttggatcct ctgcaataac tccgcctgca gccatacctg caatagcaac tccccatgca
     3181 cttccgattc ctgccataga tgcattagca gcacctaaaa tagctaaaaa accacccatt
     3241 gtaaattcca tttttaaatt tcctccttaa ttttaatata tttagttttt ttagtgaaag
     3301 gagcaaatgc atctcctcca ccttcataaa atttaccgaa aaattctaca tattgaagcc
     3361 ttgccgagtg aacgaatgaa ccaagaccat ttatggcaaa atttagtgta tggccgaata
     3421 caaaaaccaa tacatacata attgcacctg ttatgccgct tcccatggag ccaatcaaac
     3481 ttaaaacaga tgaaataact cctgttgcca gtccaagtgc aagaagtctt gaatatgaaa
     3541 gaacatctcc caggtagcct gttattccat acaaggcaag cactccgctt ataagtttgt
     3601 tgattatacc tacctttgcc cttccttggg taaggacgag tcctattgct gaagctattg
     3661 tcacccattt acctataaca ggcaatacag aacccggaat tatcatatct cctgcaagcc
     3721 agaatatagg tcctaagata aacaaatacc aaagacccac atccataagt gcatccgcaa
     3781 ggtggccgtc tcttattgac atataggcgc tcattcccat tcctacaaat aagtggacga
     3841 gacccataac aaaagatact atcagcacaa ccataggttc ttttaggggg tctacccata
     3901 cgtaggttat aggcagatta atattgaaaa gatgtcttgc tcctacaact aaaccatccc
     3961 caaaccagct tccgtacata actccccaaa aaatggtgga tattccgccc cagaaaaaca
     4021 ccttgagcat tctgccaaaa gtgccttcta tatcaaactt tttgagtatg atgtagcatc
     4081 ccagagcaaa cataacacca tatcccacat ctcccaacat cataccgaaa aacaaaacgt
     4141 aaaacggtgc catcactgcc gtcggatcta tgttggatgt tgcaggcagg ctgtagagct
     4201 ctgttattga ttcaaagggc tcagcaaaag cattgttttt caataaaatt ggaaagtttt
     4261 ccccttcctc gggcttttta aaatcatacc agcactgata ttggtctaaa accttttgaa
     4321 cactgtcttt gcatgcttca ggcacccagc cttcaacata aaaggttgta tcagtcttaa
     4381 gcatgctgct cagtacttta tttttatctc tttcaattac cagatggtca tgatacagct
     4441 gaatttcttc tttgaaggat actaattccg ttatatcttt atccacagtc tccttcttgg
     4501 cagatatttc ttttaagctg ttttcataca gcattatatt ttcttctgca gtacccctta
     4561 ggtccttgaa tgtaacagga gaaaaaccat attgtttcag caattccaaa atatcctctt
     4621 tttcttcggt aagacatata aatgagcaat aatactgatc cttgtcactg tccaccacat
     4681 ccatataaca tctttcagtt gttctaaaca gtttggattt cagctcatta atatccgcaa
     4741 tactgggtac ggcaccgatt attacgctgg tgtacttagt ttctgtcatg tcaagaggaa
     4801 tcggatactt aatccatggc tttaagctta aaatagctgc ttcaattttg tttccctcag
     4861 aacaaagctc gcttaatgac ttgtttaatc caagaactct gttcacgttt tcagatacgc
     4921 tgtcattttt ctccaatgcc tttataaatt cggctgaaga gacaggtttt cttgtattaa
     4981 ataatggtct tttgcttgtg tcgtactttt caaggctgtt cagtacttcg gaaacttgtg
     5041 aaattttcac gtccaaattg aaaacttcat tttcattgcc atcctttttt acatagctat
     5101 tccattcagg gtctgtaagt ttaaaatcct gggagcttat ttcaacaact cctaaatcca
     5161 taattctttt aataagctct gtttttatac tgtctaaacc tacgatgttg attttgttca
     5221 tattaactat tgtcactggt cttcacgatc ctttccagta taatcgaagc tgccttgtcc
     5281 atgttttggc ctgattttgt taatatttcg tcacaatccc ttgcagcatc gttaataatg
     5341 ccgtcataaa gaagttgtcc ctcggattct gctttgctta atacttcaga tctgtcagat
     5401 tctgcttttt tccttgcttc atccaagatt tcttgagctt ttgcattagc attggtaata
     5461 acctgtcttg cttgtgctgc ggcatccttt ttaattgctt cagctttttc ctccgcctcc
     5521 tttattacct taattacttc aattgacact ataccaccac ctttatgata atttttaata
     5581 aatctaacac tattccatat tgcttaataa taagataaaa acgatagttt gtcaagtttt
     5641 tatctaaatc ggtaaatact aaaagaagaa catttttgct tttttattac ttaagtaatt
     5701 ttttataaat cattgtttat tattgttttc tccttaacaa ttatctattt acaaaagtaa
     5761 aatttgagag ttttaaactt gacttttata aaaataacct cctattagga ggttaaaaat
     5821 aatgataaaa atatattttc ttgatataag taggggtcat tctacctgct tgtagaaaat
     5881 ttggatagaa catcaaggga atcaaccagt ctctccaagg actgggtttg caactattcc
     5941 actgcaaaaa taagtgttgc ttctgctgca atttccccat taacataagc aattgccgaa
     6001 ccgattccaa aggttgattt cagctttgtt atttctgtaa ccagcttcaa tgtatctccc
     6061 ggaagaacct ttcttttgaa ctttgcactt tgaatcccgc caaaataaac cgtcttgctt
     6121 ttaaattttt ctaaggacaa tatggcaaag gctcctgtct gcgccagtgc ttcaatgatt
     6181 aagacccccg gcattacagg gtttccggga aaatgaccct gaaaaaactg ttcattcatt
     6241 gtcagatttt ttattcctac agccattttc ccttcttcaa gctcaataat cttatcgatt
     6301 aaaagcattg gatacctgtg gggaattata ttttgaatat cgtttatatc atagttcata
     6361 atactatcct ttgtattttt ttaaaaccaa tgttgcatta tgacctccaa atccaaatgc
     6421 atttgacagt gcataatcaa attcttttga aattccaaca ttaggcacaa aattcaaccc
     6481 cagttccggg tcagtctccg catgatttat ggtggcagga ataaacgaat cttctatggc
     6541 tttaacacag attattgctt ctacagctcc tgccgctcca agcaaatgtc ctgtcatgga
     6601 ttttgtggag cttacataga tattctttga atcttccttg aaaacattct taatcgcctg
     6661 ggtttcaaat ttatcattgt acaaggtgga tgttccgtga gcatttatat aggttatatc
     6721 ctctggctgg attcctgcat ccaaaatagc atttatcatt gatttagtgg caccttcaga
     6781 gctggggtcg gggcttgtca tatgataagc atcgcatgtt gctccatatc cggcaatttc
     6841 tccatatatt cttgctcctc ttttaagagc atgctcataa tcttcaagaa ccattaggcc
     6901 ggcaccctct cccattacaa aaccatgtct gtccttgtca aaaggtatgg aagctctgtt
     6961 tatgtctgtt gttgttgata atgtggtaag aacagtaaat cctgctatgg agcagggagt
     7021 tattgcagct tcagcacctc ctgcaatcat aacatcgcag attccatcct gtattctttt
     7081 gtatgattct cctattgcat tggcacctgt tgcacaagca gtcacagtac ttatattcac
     7141 agccttggcc tggtatttta cggcaatcag tccggatgcc atatttgaaa gcatcattgg
     7201 tatgaaaaat gttgaaacct tgccgggccc ctttgtcaaa agcttttgat gctcttcttc
     7261 aattgtatga attccgccta cggcacttcc aacaatacat ccaaatctgt cagaatcaat
     7321 tccctcagta tcaagaccgg aatcctttac tgcaatatct gcagcagcga gggcaaactg
     7381 gctatatctg tccattcttt tggattcctt cttttccatg tataaaacag gatcaaaatc
     7441 cttcacttct gctgctaatt taacgctgaa gtccgacgtg tcaaaggatt caataaaatt
     7501 aattccgcat tttccctttt ttacattctc ccaaaaaata tctcccgtac caatggggct
     7561 tactactccg attcctgtta taacaacttt tcttttcatc taatctcctc tttatctaat
     7621 caaaaattaa ttttttaagc ggtttgcaaa acacctcgca gtctctcagt ctcaatcacc
     7681 aatttatttg ctccctctgg tcgcataaat tgggatttcg ttgcgaatga cctaccgcta
     7741 tttttcagaa aaaatactct gaaaaatagg gttagggcat ctacatataa aaacccccgt
     7801 taacagaaat gacctggcct gttatatagg atgctttgtc cgatattaaa aactcaacac
     7861 aatcagctac atcctccggc tgtccaagtc tttgaagtgg gatgtttttc atcatttcag
     7921 cctttatctc atccggaatc ttatccgtca tttcggtttt aataaatccc ggtgccacgg
     7981 catttaccag aatatttttc ttacctaatt ctcttgctat ggactttgtc atacctatta
     8041 ctcccgcctt tgaagctgaa tagtttacct gtccaggatt tccggcaagg cctgacacag
     8101 acgatatgtt tataattctg ccttgttttt gctttatcat tcttcttgca atatgtttgc
     8161 agcagttaaa ggtacccttt aaatttgtgt caattacctg gtcgaattcc tcctctgtca
     8221 ttaaaggaag tatattatcc tttgtaattc ctgcattatt aataagcaca tccacagtgc
     8281 agaaattatt gtaagcttca tctattagta tttttgcatc ctcataaaca gaaatatcac
     8341 atttaacaat aatatttttg gtttctctat tagtaaaatt ttctatcagc ttttccattg
     8401 aagctgtgct gcttctgtaa ttcaaaacta aattataatt cagctcgttc agtctttttg
     8461 caagaacagc acctatccct ttggaagcac cagttataat tgcagttttc attattgcct
     8521 tccttttttt taagctcctg caaattgcag gtatgaattt aacccttgga cgctatcgac
     8581 atttattgat tttacatttt tatcaatttt cttaataaat cctgtaagtg tttttccggg
     8641 acctatttct acaaatgtgt tcacaccgtc tccaatcatc tttctgacgc aggcttccca
     8701 ccttacagga ttatagacct gcttttccag cttttctgca attttaccta tttcgtaata
     8761 taaagtatcc acatttgcta taacatcacg aacaggcctt ttgaatttaa tttttgataa
     8821 atcttcttta agtctgtctc ctgcaggttt aagcattgat gtgtgaaatg gtgcactaac
     8881 ctgaagcttt atggctcttt tgatattaaa accgggtgct ttatcaacag cattatcaat
     8941 ggcagtgctt ctgccgccgg ttactatttg accggggcaa ttaaagtttg cgggttccag
     9001 caaaccatat tttcctgtta tatcattgca gaaagcaatt gcatcctcat ccgtacaccc
     9061 caaaagagca agcattcctc cttctcctaa aggaactgtg ttttgcatat agtttcctct
     9121 tttataaacc aaccttacag ctgtttcaaa gtccattgca ccggctgcag tcagggcaga
     9181 atattcgccc aggcttaatc cgcccattac ggcagaattg tcattcttct ttaaaacttc
     9241 ataaatagca aggcacactg ttaaaagcgc cggctgggtt atttttgtaa cgctaagttc
     9301 ttcatcaggc ccttcaaaac aaagctttgt tatgtcaaat cccaatgctt cgttcgccct
     9361 gtgaaatatt tccctgcttt ccttaaaatt gtcataaaaa tcttttccca tgcctgcaaa
     9421 ttgagccccc tggccgggaa aaacaaaagc tgttttattc attcctgcct ccttggctga
     9481 ctattaaatt cttcataact ttttctgctt cttcaaacat ttcactgata atttccatac
     9541 aggtctgctc tttttttacc aaccctgcaa tttggcctga cataacagag ccgtaatcca
     9601 catcgccttc cacagctgcc ttgtaaagct tccctctccc caattcctcc atttcctcaa
     9661 cagctgcatt attcttttcc aagtccttga attgagttac aagcctgttt tttaataccc
     9721 tgacaggatg ccctgtcttt ctgcctgtaa cggcagtatc tgtatcgctt gcttttttta
     9781 tcttgtcctt gtaattttga tggaccctgc attcctttgc cagaacgaat cttgttccaa
     9841 cctggactcc ttgtgcacct aggcagaagg ctgccgcaat cccccttccg tcacctattc
     9901 ccccggcagc tattacggga atactgacag catccacaac ctgaggcgtg atgcacatag
     9961 ttgtaatctc gcctacatgg cctcccgctt cagttccttc gcaaattacc gcatcagcac
    10021 ctgccttttc aaaatacctg gcaaaagcta cagagggaac aacaggtatt accttgattc
    10081 ctctttcctt ccatactttc atatacttcc ccggattgcc cgctcccgtt attattactg
    10141 gaaccttttc ttctattaac aattcgctta cttcctgtga ataaggactt aaaagcatta
    10201 cattcacacc aaatggcttg tctgtcattt ctctgatttt atgtatttct gcttttattg
    10261 actcaggagg attgttgccg gatgctatta tacccagccc tcctgcttct gatacggctg
    10321 ctgcaagccc tgcatcggat atttgtgcca tgcctccctg aaaaatagga tattttatat
    10381 ttaatagttc acatactttt gtataaatca taattctcct catagcagaa aacgccttgc
    10441 ggcgtcttat taaatttgtt ttctttctgt aaggtaattg actatatcgc ctactgttat
    10501 tagcttaccg taatcttcct cttcaattac cacatcaagc tcatcttcaa ttttatttat
    10561 cagttcaaat aagtccaatg agtctgctcc cagatcgtcc ttgattttac tttctaaggt
    10621 gatgctttct tcttccacgt ccaattcttc aactaaaatt tccttaacct tttctaaaat
    10681 cataatttcc tccatataaa tttactttga ttgttgtatt tatttatcct caaatatttt
    10741 gacattcaaa gtataataca atataaatcc cttgtcaata tattttttat gacaagggac
    10801 gtagactacc agtttgtttt tatagcttct acaatgcggt ctgcggcctt tccgtctccg
    10861 taggggttta ccgcattggc catttttttg tattcatctt tattattgag cagtatattt
    10921 agctccttga aaatattatc cctgtctgta cctgctaatt tggcggttcc tgcctggatg
    10981 ccctcaggtc tttctgtctc tctcctcaca accaggaccg gcttgcccag tgacggagct
    11041 tcttcctgca cgccgcctga gtctgttaca acaagatagc atttattcat caaatttgta
    11101 aaaggaagat aatccagggg ctcaatcaaa tgaacacttt ccatcccctt gaatatttta
    11161 tcggcaattt ctctaacctt aggatttaaa tgcatgggat atataatttc aacatcctta
    11221 tttttttctg caatatcttt cagggcagaa aatatatttt ccataggttt gcctatattc
    11281 tctcttctgt gggcagttaa aagtattacc tttttatttt taaaatctat attattgata
    11341 atttcttctt caaatttata attttcatca ataacccagc ttaatgcatc aattgaggtg
    11401 tttccggtta taaatatttt attttcgtca tagccttctt ttaaaagatt atccctggat
    11461 gtttctgtag gggcaaaatg aaaatcagtt aaaactcctg tcatcattct gtttgcttct
    11521 tcgggatacg gtgaatacag attgccgctt cttaatcccg cttccacatg acctattttc
    11581 acctgatgat aaaatgcagc aagagcacct gaaaataccg ttgttgtatc tccctgcacc
    11641 agtattaaac cgggtttgaa atcttctatc acttcttcca accctgtcaa tgctctgcag
    11701 gtaatttgtg taagtgtctg acctctttca aaaatatcca aatcatagtc aggttcaata
    11761 tcaaatattc tcagaacctg gtcaagcata tccctgtgct gggcagttac gcagatttta
    11821 gattcgaaag tatccttatc tttttttaaa gctttaacta cgggggccat tttcaccgcc
    11881 tccggacgag tgccgaatac taccaaaacc ttaattttat ccatctgctc ttttcctcct
    11941 atttagcagg ggaatccaca aattcaggtg tgtcccgcac ccctgacctt gcatagttta
    12001 tctttgacct gccgtcaatt tcacattcga agacattcct catgcccaaa tacttactca
    12061 ataatagtgg tgtacccaat acctgcatcc gaagaaattt ggccgggtaa taactaatgt
    12121 tcttttctca caaaaagccc tgctttttta gcaaataaaa atgcaattat tacaactgca
    12181 gttgcaaccg ccaacccggt gaaggggtct gcatctgcta ttattaccga aactattccc
    12241 aaaagcatgc ttataaaata tattattaaa actgtctgcc tctgattgag acccacaccc
    12301 ataagtttat ggtggagatg gtccttgtct gcctccatta taggtttttt attgatatat
    12361 cttcttatta ttgcaaatgc agtatccagt ataggcacac ccagtgcaaa cacgggaacg
    12421 gccaatgcaa tcacggcaac actcttaacc atacctaaaa cagatgtaac cgccagcata
    12481 tatcccagta acaaggagcc cgtatctccc atgaatattt tcgccgggtt aaaattaaaa
    12541 ggcaaaaatc caagagaagc acctgcaata attgcgcatt gcatcacaat gaaatcataa
    12601 cccagccttg ctgcagtaaa taaaagagta gtggctgcaa tgctggcaac ccctgaagca
    12661 agcccgtcca gtccgtcaat taaatttatt gtatttgtaa tacccactat ccaaactaat
    12721 gaaataaaga aggacagcca gtcaaataag tagcttatgc cggtttggga aagagggttt
    12781 gtgataaaat gtatttgaac tcctccccct aaggctatta atgcagcaat aatctgtatt
    12841 agaagtttta cttttgcaga gatgccgtac atatcatcaa caactcctgt cagcataatc
    12901 accgttgcac ctgccattat gtggtagttg gttgaattgt ggggcatgta aacaaacatg
    12961 catgccatta ttgatacata tattgcaagt ccgccaaagt aaggcattgg tttattgtgg
    13021 atttttcttg cttcctttgg aaaatccaat gctcccacct ttattgcaag ccttcttgcc
    13081 catggggtca taataaatga tataataaca gcagatatga aagctattaa attggcagtc
    13141 atcatttttc ctcttctttg gaaatttcca tatccattat tttgttaacc ctgatttcat
    13201 gtcttccacc ttcaaattca gttgaaagcc atacatcaac catatcttct gcaagcccta
    13261 cgccggtaac cctgccgcct atagcaagca tattggcatt gttgtgcatt cttgacattc
    13321 ttgccatata ctcattggta cacaaggcac atcttattcc tttaaccttg tttgctgcaa
    13381 ttgaaattcc tattcctgtt ccgcagataa taatacccct atccgcctct ttgccggcta
    13441 ccatcatggc agctcttttc ccataatcag gataatcaac ggactttgtg tcattgacgc
    13501 ctatatcatt tatatcataa ccctttttta ttaagtgctt tttaatggct tcctttaatt
    13561 caaatccgcc gtgatcgctt gcaagtacta tcttcatatt tcctccagct tatctatgtt
    13621 tttaagaatc tttcctaatt ctttttcaat ttcatctctg ctttttttat aaaaggagta
    13681 atcctttcca aaggggtcat caacgtcggt gcctgttgat ttttccccgc ttaatttggc
    13741 tgcaaattcc ttcaaagtga aaatacgagg tgggctttca tctgtcctgc gcttaaaata
    13801 atcctcaatg cccggtttat ggcttaaagt cattgttagt attaaatccg ccccattaat
    13861 ttcatcagac agcttttttg ccctgtgcag gcttatatca attccctttt cctctaatgc
    13921 ccttattgct tttccattag ccctgtctcc cggaaaaaca ctaaggcctg cagaagaaac
    13981 accaatatta tcaatattgt ttttccttag catatccttg aatattccct ctgccatggg
    14041 actcctgcat gtgtttccag tacaaacaaa taaaatattc atcctatacc tcgattattt
    14101 ctcccgctga cttgcccagc ctgttcatta tagcctttcc cagacccttt tcatcaactg
    14161 cctctgcaag tatcacatca attcccatat ggtcgaaatc tctcaagcct ttaaacaagt
    14221 ttgacgaaat ggtaaggagt tttgttctgt ctcctatgca aatatttact tttcccttat
    14281 aaagttctct cgtttgaact gttgacataa ttcccgcctt aagtccctta agagttagct
    14341 tatcataatc ctcattgatt ttttctgcaa ctttttcaat atgcccttta tacaaaatta
    14401 ccttggcttt tggagaataa tgcctgtact tttgtcccgg tgattttgga ataattgctt
    14461 cttcgctttt tattattgaa gggtcatatg tacattctcc aagtacgctt ataatgtcct
    14521 caagggttac tccgcccggt ctaagtatca tgggaatgtc gcctgtcata tccacgactg
    14581 ttgattcaag cccgatataa gtacttcctc catcaataat catatcaacc tttcccatca
    14641 aatcttctat tacatgggag gcttccgtag ggcttgggct gccggatgta ttggcagaag
    14701 gggctgcaat tggttttttg ctttccctta tcaaggcaag tgcaatttta tttacaggca
    14761 gacgtatggc tgccgtattc agccctgccg taattttgtc agataatatt ttccttttat
    14821 atagaatcat cgtaagaggg ccgggccaga atctttcagc cagcagttca gcttttttgg
    14881 gtacttccga agccaaacag tacaattcat caagctctgc aatatgtact atcaaaggat
    14941 tgtcctgagg acgtcctttt gccttgaaaa ttttatccac cgctgcatca tttaatgcat
    15001 cagcacctat tccgtaaacc gtctctgtag gaaaaactac aagacctccc ctgtttataa
    15061 tatctgcagc ttccttaata atattataat ctatattttc aggatcaacc tttactattt
    15121 tcgtaattat gccattttcc attatatacc ttcttattga ggaatgttcc cgaatagtat
    15181 tatataacac attaacttta tgtacaagaa tttactgctt aataaatttt aatttctgaa
    15241 attgaattgc acgcatatac atataaggaa ggaggacacc cctctaacaa gaggcgggct
    15301 tttgtggcca taagaaagtt cccggtatta aagcgacccg cagcgggcat ggtgaatgtc
    15361 tttttgcatg gaggatttag gaatatcccc gtcctgggaa tacaaggagg tactgtgaat
    15421 atacttgatt atattataat gggatttgtt tttgggggaa tcgggacctc gataggagga
    15481 ttgatttccg tattgatata taagccaagc gataaaatgg tttcaggaat tttaagcttt
    15541 gctgccggaa ttatgctggc ggtaacatcc ttcgacttga tgcctgaagc ctatgaaatc
    15601 gggggattct tcatagttac cctgggtctt tttataggcc ttataattgt attttttaca
    15661 aaggacatga ttcctgcgga taagctgaaa caatataagg gtaaaaagct cagttacata
    15721 aaaatgagct taataataat aatttccata agcctgcaca atttcccgga aggtgtggca
    15781 gtgggctcca gctatgttta ctcaaatgat ttaggtgtaa aaataggcat actgattgca
    15841 gcacatgata ttcctgaagg cataagcgta ggtgttcccc tggttatggc aggggtagcc
    15901 cccttaatgg taattatcat cactcttctg acaggccttc ctactgctct tggagccgtt
    15961 ttcggagcat tggcaggagg aatatcagaa tatttcatag ccttttgcct ttcagctgca
    16021 gccagcagca tgctttatgt gtcggccaac gaattaattc ctgaagcata tctgctttat
    16081 aacggaaaaa gcagctcaat tatgctgctt ttaggttttc tgtgcggatg ctatatatcc
    16141 tttggaattt aaggtatggg gacacatctc tattctgagg taagtctctg cgggcacgag
    16201 gaatgtcccc gtcattatgg ggacacaccc tacaaggtac cattctattt gatttcaaga
    16261 tccagataaa ttttttcata tactcctttc agcagtgagt cgattacttc attgtcctct
    16321 ttcctgaatc ttatgctgat tccacagtca gaacttatgc ttcttggtac aggtatggtt
    16381 ttatattcaa gattgttttt cttaagaatt gattccgctt tcatggcaaa tgaaactgat
    16441 ttaaatgtca tgtaattata tttcatagct ctgccttact ccatttcatc caaggtaagg
    16501 ctgtagcctt ccaggaattc atccataaat atttcaactt caggcatatt catgcttttt
    16561 attgcagaaa gcaaagtctg ctttgctttt aaaaattcct tgttgcccga tttttcttct
    16621 tccaggcact taatatatgc gcttatttta tctgctgcct ttatggtctt ccatatttcc
    16681 ttctcttctt ccttaggaac cagcacatct tcaaactctt ctttaaaatc accgggaagc
    16741 atatttaaca gtctttcatt tactgacagc tccacatctt tataagcgga cttaattaca
    16801 gaattgtaat actttaccgg tgtgggcata tctcccgtaa atatttccga cacatcgtga
    16861 taaattgcat aaagagctgt cctttccgga tttacttcca tattcaaccg ttttttttgt
    16921 attacagcca aagcatgggc aataacagca acatcaaggc tgtgttcaga tacattttcc
    16981 ttattgatgt ttctcatcaa accccagcgg tcaatatatt tcattctgga tatgaatgca
    17041 aaaaaattag actttttcat attatcacct gcatattcgg acatcttaaa tgtaagtacg
    17101 gttcagcacc cacaagcaat gtccttttaa tacagtaatt ttagcagata atgctcatta
    17161 tatcaacata aataaatgag atcccccagt ctctatgaac atgggattac atgttgtcat
    17221 cccatgcaaa actgtgaaat ctcatttttt ctaatgcctc taataaatag gggtcgctcg
    17281 aagaaatcgg gttagagcat cttaatttat cagcagctca ttgacctttt tatttgtatc
    17341 tatactgtga acctgcattt cattctcgga gaatgtgtac aaataatccc caatatagat
    17401 tatccttttg atattatcca aataggaagc aaattccgac tcattgtgac ttattgtact
    17461 ttttaatttt atgctgtcat tgctgatatt atatacatat gccccgttaa agtcgctttt
    17521 ataattttct gccatcctgc ttaccggaaa tgccatcaat cccttattca aggaaaacat
    17581 cagcgccttg tgattgtaca gcagttccga gtacgttccc gccttcccta ttacctccgt
    17641 gaatgtttcc tttggtctat taacattgga aacatcaaac atggaaattt tcaagccgcc
    17701 gttaactgtt cctccccatt ggttttcttc tgtatcataa ccgaatccca ggatgtggtt
    17761 ttcgtcaacc atatgaagat atgtgctgta tccaggaatt ttaagcattc ctttcaattc
    17821 aggagctctt ggatttgatg tgtcaattac aaacaggggg tctacctgcc tgaatgtaac
    17881 catgtaaact ctgtcgcctg caaaccgggt gctgtaaatt ctttcccctt cagccaaacc
    17941 ttccagtttc cccaatgctt ttaagttttc atccaagata tatacattgt ttttggatgt
    18001 gttcatccac atttcacctg tggtagttgc aattctaaag gtgttgttat gctcatccat
    18061 tgagaactgg ttgattatgg ttcccggcac ctggccttgg acagctgtgc cgatatttcc
    18121 cccatccagc ttaaatttat agattaccgt acttgttgta tattcaggat taaactcctc
    18181 catctgaggg atttcgtaat cgtatgaata gtctgttact gcagcgtata aattatcctt
    18241 tgaaacataa attgtatccg tacctcccaa gtacacatcc acgtcaggtt tagacacctt
    18301 gtcggataaa tcaattccta tggtgtacat gtatctggtg tcaatataat ttggaaaata
    18361 ctttatttgg tcatatccga attcatgttt ctcaccccta agcacatcag tataataagg
    18421 aagaggtatg tctatatcaa tattgtcata gtacctgtat cctaagttga gatacttgtt
    18481 gttcaccaaa taaaaactgt catcaataac ccttcctgac aaatagttgc cgtcaaaaag
    18541 atattctctc tcaagcaccg gtttttcaat attttctata tcataaatca atacatctgt
    18601 cctgttctca tcgtatcttg ggggcattat caaaggttta gtcagatccg ggctgtcggc
    18661 agcaggcttt tccataatat agtaaaaatt gttctgacca ataacaatta acttatttcc
    18721 ggcagtatat atttcactta tgcttgtatt ttcgggaaca aaaattgtac ttacaacttt
    18781 gggagctttt ggatttgagt caattatctt taaccccctg tctgtaataa catatatgta
    18841 tctgccgtca ttttttatga tatccccttc ctgaacacct tcaacctgaa catttgtatc
    18901 agacttatcc atgttggaag taccggaatt ttctgcctga gtttcctgga cagcaggggc
    18961 ttcagccgct gagtcttcgg caataccgcc aacccaagct ctgtccatcc aaatataatt
    19021 gtggagtttt gaattatact ccaataaagc aatcaaattt tcctttgttc caacggttga
    19081 aattccttta tcaacaggga aagtatcatt ctttgttata taaaccgagc ttgtgtttcc
    19141 gtcccacctc actgtgtgtc ctaaattttc agctgcaaat ctaaggggag caaatgtcct
    19201 gctgtttatc atcctggtag gcacatcgat atccttgatt atgccgttaa ccattacctt
    19261 gtccttgcct aaaatcattt ctatctcatt attgtcatcc ttaattacca cttccaaaag
    19321 gtctttgttc cagtcaacct ttgcgcccag tttttcaaaa acacccctta agggtatcag
    19381 ggtccttcca ttctcaatga aaggctgtac atcgaaatcc accttttcat tatccacata
    19441 aacctttatg tcctgagcaa aagctgtaaa agtaaaacat aaaactaaaa tcaaagtcaa
    19501 caaaataatt ttataagttt tcttcatata ttctcacctc tcttttctta gacgaattta
    19561 aataacatct tgttcctaat tataccataa tatttttatt ataaacataa taaaaaagat
    19621 ggctggtcac catcctttta tgtgtatgtg cattttttta tcttgtatac tccgcagggg
    19681 aatccatcaa atttctttgc ttcagctttt aaatgtgctg cagcaggttt cctccggaac
    19741 acttgcattc tttccgtt
//
LOCUS       DAOPUT010000020        19184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_2903_length_19184_cov_19.382979, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000020 DAOPUT010000000
VERSION     DAOPUT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 19184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19184
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2903_length_19184_cov_19.382979"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKK75_03395"
     CDS             <1..216
                     /locus_tag="PKK75_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNZ82671.1"
                     /translation="LNYEGKGYAKLKEDVAEVVVEELKPIQERIDYLLKNKDYLEEIY
                     SIGAQKAEVAARKTLRKVQKKVGFLPR"
     gene            239..>1182
                     /locus_tag="PKK75_03400"
     CDS             239..>1182
                     /locus_tag="PKK75_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNZ82672.1"
                     /translation="MLVIKNGYIKTMDGEDIEKGQIIIENGKIKAVGRNLDIPEDAEI
                     FDAEGLLVTPGLVEAHCHLGMFEEGIGFEGDDGNESVEPITPQLRAIDGINPMDQGFE
                     DAREGGVTTAVTGPGSANVIGGTFAAIKTYGKKIDDMVIKEPVAMKVAFGENPKRVYD
                     EQHKSPVTRMAVAALLRETLFEAKKYMEDLEASKEDPDQKPDFDIKLHSMMPVLKKEI
                     PLKAHVHRADDVFTALRIAKEFDLDITLEHCTEGHLIVEELKAAGKPCLIGPTFGPRI
                     KVEMKNKSFETPKMLADAGIKTAIITDANVIPIQHLTM"
     assembly_gap    1183..1282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1414..3228
                     /gene="typA"
                     /locus_tag="PKK75_03405"
     CDS             1414..3228
                     /gene="typA"
                     /locus_tag="PKK75_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNZ82673.1"
                     /translation="MKKKIINIAVIAHVDAGKSTLVDAFLQQSGVFRENQAVIEQVMD
                     SNALERERGITIYSKNCSVMHNGIKINIVDTPGHADFSSEVERIMKTVDTVILLVDSI
                     EGPMPQTRFVLHKSLEIGLRPILLINKIDKKNHRAQEVVDMVFDLFVELNANDEQLDF
                     PILYGIAKQGIVQYELDEESDSIIPLFDTIIKHVQPYPDRDEENLQMQVSTLAYDDYI
                     GRLGIGRITAGTIREGQMVSVAKHDGSVEKQRISNIFIYEGLRRTLVNEAFSGDIVVV
                     SGISNISIGDTICSENNVIPMETIKIEEPTMSMNFLINTSPFAGLSGKYVTTRHLKSR
                     LEKELEVNVGLRVEALEGALDGYKVSGRGELHLSILLENMRREGYEVSVSRPEVILKR
                     IDGLVMEPVERVVVHVPEIYSGSVISKLNLRKGMMVSMMPDGNYVKIEYMVPTRGLLG
                     YRTELINDSRGEGTMVRIFDHYEKFKGEIPQRTNGVLISNDQGEAIAYSLDNLESRGI
                     LFINPGENVYEGMIIGMNSRGEDITVNPCKAKRQSNIRSSASDDAIKLSPPRIMTLEA
                     ALEFINDDELVEITPDAIRLRKRYLTEMERRKQGNKKP"
     assembly_gap    3236..3335
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3395..4789
                     /locus_tag="PKK75_03410"
     CDS             3395..4789
                     /locus_tag="PKK75_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ82674.1"
                     /translation="MKAKECLLLMQKILQLVDEGVHVLDKDGNTIIYNESMSALEKMK
                     SCDVMKKPFTEVFKELNSENSTLLKALHSKIITKNLKQTYLNKDGKEITTINTTIPVI
                     MDDEVIAAVEVAKNITKIEEMSNTILKLHDEIEKPDKIKNKKVKKYNFENIVGQSDNF
                     LDVIDKAKKSSKNTASVFIYGETGTGKELIAQSIHYASSRKDKPFKAQNCAALPESLL
                     EGLLFGTSKGGFTGAVDRAGLFEQANGGTLLLDEINSMPYELQPKLLRVLQENYIRRV
                     GGSNDIPIDVRIITTCNEPPEKILREGKLRKDLFYRLNVIPLNVPPLRERINDIILLA
                     KMFTDKHNIRFNKHVTDFSDEVYDYLLSYDYPGNARELENMIMSAVSMCEDDEHILDI
                     GHFSIVHSKDHKINNYDRVESLGMDKYLSDIEKKIIEKALLDNDNNITRASGALRIKR
                     QTLQHKIKKYKINF"
     gene            5040..>6076
                     /gene="ord"
                     /locus_tag="PKK75_03415"
     CDS             5040..>6076
                     /gene="ord"
                     /locus_tag="PKK75_03415"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HNZ82675.1"
                     /translation="MQNVKVIIWGLGAMGSGMAKMLLSKKGVDIVGAVGRGAKIGKSM
                     YELIGVERGDRPEVLVGAPEDVIKEKAADVVLCCTDSYTKVAFERLKFCLEKKINVVS
                     SAEEMSFPMAQSPEEAKQLDEIAKKNGVSIIGTGINPGLIMDLLVVVMTGCCESVDHI
                     VSRRVNSLSPFGPTVMHEQGIGITVDEFKKGVETGTLAGHVGFEESIGMIASAIGWKV
                     DKVTTSMDPIVTDVDRKSQYGFAKAGNVAGVAMKGFGYIDGELKIEMDHPQQIEPEQV
                     GVQTGDYVIIKGTPNINMVNSPEVPGGIGTIAMCVNTIPHIINAKPGLHTMLTIPVPR
                     AIMGDMRDLIDP"
     assembly_gap    6077..6176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6205..6510
                     /gene="ortA"
                     /locus_tag="PKK75_03420"
     CDS             6205..6510
                     /gene="ortA"
                     /locus_tag="PKK75_03420"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HNZ82676.1"
                     /translation="MVKKGEWVLIHRNVLEPSERAPQVPDDTKKVPLELWTKGFLQED
                     ANIGDEVTVLTRTKRIEKGTLLEANPYYKHDFGKFVPELLTISDQVKEILFGGADNE"
     gene            6503..>7368
                     /locus_tag="PKK75_03425"
     CDS             6503..>7368
                     /locus_tag="PKK75_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HNZ82677.1"
                     /translation="MSKDMTYAGVMARRPEIMKNSAGLDFSKFESGSIAFDYERMMKE
                     AGFTIEEIQKIQSEHGVGNTPIIELKNLTALARKVAPEGKGARIFIKDEASNASGSFK
                     ARRASTAVYQAKKLGYKGVVTATSGNYGAAVASQAAMYGLKCIVVQECYDSRGVGQPE
                     IVEKARKCEALGAEVVQLSVGPELFYIFLSILEETGYFNASLYTPYAIAGVETLGYEI
                     AMQFREKFGKDPDVVIATNAGGGNLTGTARGLRKAGAQSKIVAASVDLSGLHMAADTQ
                     FNKKSFTTGHTG"
     assembly_gap    7369..7468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7702..8052
                     /locus_tag="PKK75_03430"
     CDS             7702..8052
                     /locus_tag="PKK75_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HNZ82678.1"
                     /translation="MKRADDYQQRRAHLEGLSDEELYERFWKLANEIVDPLLELGRKN
                     TTPSVERSILLRMGFSSIEVKPIVEGVMNKGLMGKGAGNVVWRLSKKLGCSVREAGLL
                     LADGKHWDDVDTLF"
     gene            8065..10260
                     /gene="oraE"
                     /locus_tag="PKK75_03435"
     CDS             8065..10260
                     /gene="oraE"
                     /locus_tag="PKK75_03435"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HNZ82679.1"
                     /translation="MKLEPGKKLDVKELLKDLDKYEPRRRGWHWREGRNEKRKIGDFE
                     YHETSESLKNSIPLPGSRGFGYIDPQPDCVITTEIASGRFEDDIRRMRMAAWHGADHL
                     MVIRTAGQSHIDSLLEGTNQGIGGIMVTRKQVRASRKACDLIEDEIGRPINFHSYISG
                     VAGPDIAVMFAEEGVNGAHQDPQYNVLYRNVNMVRSFVDACEAKKVMAWADMLQIDGA
                     HNANATAMKAWKVMPELMVQHAINTAFSVKIGIKPENIALSTVPPTAPPAPCMRLDLP
                     YAVALRDLFKGYKMRAQQNTKYMESDTREAAVTHTLNMVISRLTSADIQSTITPDEGR
                     NVPWHYNNIAGTMTAKQALTGMDGFREMVKLDRDGPLGDKVRELKERAILFMEEMIEV
                     GGYFKAVEEGFFIDSGEYPERNGDGIPRQIEGGIGYGFIYERDEDYFAPVSVHYGYNN
                     IPAEFEKASDAIGGDTFEDRSKIQYIDELDEHDNVNVRLNEVEKYYDTNLVKPEVEWR
                     ADGTVLLTIFLSLDERTAEFAAIKCGEKMGLEDVTVVHKQSIHPSEGTYCEVKGKVNF
                     DIDINELVIPEKPDILSEAEIREDIQNKPMFVVAATVGEDEHSVGLKETLDIKHGGIE
                     GFGIKYEYLGTSCPVEKLVDAAIETNADAILLSTIITHDDVHIKNMKKVHDLAIEKGI
                     RDKVMIICGGTQVTNETAMEAGLDAGFGRGTHGIDVASFLVKRRREKNA"
     gene            10260..11612
                     /locus_tag="PKK75_03440"
     CDS             10260..11612
                     /locus_tag="PKK75_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HNZ82680.1"
                     /translation="MKVNVLVAEIGSTTTVVNAFNGIGTGNPAFAGQGQAPTTVLEGD
                     VTIGLKGAIEDLKKNLQADTLTWDDMLATSSAAGGLKMTVHGLVYDMTVRAAKEAALG
                     AGGIIKMITSGRLRKTDLKKIKEIAPNIILVAGGVDYGERDTALYNMEMILSMGLKIP
                     VIYAGNIENQEEVRLMCEEADNRLYIVENVYPKIDTLVVEPTRKIIQDAFEEHIIHAP
                     GMTKVREWVKGPIIPTPGAVMEAARVLKEEIGDLIVFDVGGATTDLHSVTEGSSEINS
                     ILISPEPEAKRTVEGDLGVYVNVSHIVEKIGMDNLKKEFPDAEELLINYKPIPITHRE
                     KLFVERLTKEAVLTSLERHAGKLRYFYTASGKKTVAEGKDLTAIKYIIGTGGTLTRLS
                     GNRRILETVKTYAKEHELYPAETAKVLIDEDYILSSLGVLSKSYLNDALYLIKKSLRM
                     "
     gene            11621..12688
                     /gene="orr"
                     /locus_tag="PKK75_03445"
     CDS             11621..12688
                     /gene="orr"
                     /locus_tag="PKK75_03445"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HNZ82681.1"
                     /translation="MYPRLAIDLKKVKNNLDKITQMVKGSGSSLMIVTKGYSADMEIY
                     KLLDESNIDYLADSRIQNLKKYEGTKKGRVLLRLPMNSETDEVVKYADVSLNSELKTI
                     KNLNESAKVQDKIHKILLMIDLGDLREGIFFKNEDEINYTVEEILKLENIEIFGLGVN
                     LTCYGAVIPKKDNLSVLVDIARKIEKKYDIKLQMISGGNSSSIYLIGKDELPEGINNL
                     RVGEAFLLGGETAYGQKLEGFYDDAFTLEAEIIELKEKQSVPIGETGVDAFGNKPVYE
                     DRGIIRRAIIAVGRQDVDPDALSPMDEKIDILGASSDHLILDVSKSDNEYHVGGIVNF
                     KLSYSSLLRATTSGYVERVYR"
     gene            12914..15130
                     /locus_tag="PKK75_03450"
     CDS             12914..15130
                     /locus_tag="PKK75_03450"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HNZ82682.1"
                     /translation="MKNIKRMGIFLLLFACTLVLLLKEPFVGIADNSDYYRVIQPLGF
                     QPEISNRYFYAYNFYTVNDMSSEDINGSLSNIISPKVENDNEYFSTQFIFIKISMVIN
                     YLLKIILGKSPEIFNIKILGILYGGIYSYGLYLFLTNLKFKRRSIYILFLIISLVILC
                     DMGYLLYFNSFFGEAVIISSLMMTLGALYAFIRTEESGKSIYYGILFYVFALALTGAK
                     VANTPIGILIGLFSLTLFIIKKDRLNRTLITAGSILIICFSVFYYANAPRWMSQVNNY
                     QSIFYGITKDSKEPEKDLEKLSIPLKYLPLTNTHGFLDHGDFDIYSDEFKEEVYDNAS
                     FVDILKFYLLNPSRAMEKLKLSADSSVIIRPSYLGNYSKEDMPERLEFTQRFSLWSNI
                     RKNTLGYAFNIIAVFSVLFFIINIYEIINSINRRDNEKIVLSFAALLLFLTTISQFVL
                     PVIGNGEADLQKHMLLFNLCFDLMVLAGLNWLINNYSLKMVLKIVLTASVLLTATILI
                     QPANEKVEETGPLRTGQYVYFGTYKNEPLKWVVLNSDENGFLLWCDKPVEYMEFDNRD
                     ETSTENVYGSNDWIESDIRKWLNSEFKNNFKEEDKLFINDVRLKNILSYNNIDQSIGG
                     NKPFYWNSITSYVSQNYNTDAYYNYSAEGVFLLDAYQLEKFVYENNIDIKKDGRYWLR
                     TPYYSSASMVRIVDRDGFVYHKDANVKAGVIPAVYIDDNIRVMQGDGTYSSPFTIE"
     gene            15141..16094
                     /locus_tag="PKK75_03455"
     CDS             15141..16094
                     /locus_tag="PKK75_03455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007473672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ82683.1"
                     /translation="MKKVSLLIPAYNEEETIPLLYDELNKVLETIPGYEFEILFVNDG
                     SSDNTLNILRNMQQRDTRINYISFSRNFGKETAMAAGFDYVTGDAAVIMDADLQDPPE
                     LIGEMIKYWEEGYDDVYAKRRSREGETWLKKFTSAAFYKLLQKMTKIPIQEDTGDFRL
                     LDRRAIESLKKLREKQRYTKGMFSWIGFNKKEILFDRKPRAAGKTKWNYLKLFNLALE
                     GVTSFTTSPLRISTIIGILVSIFSMIYMVIVLIKSLIWKDPVQGYPSMMVTILFLGGV
                     QLVSLGIIGEYVGRIFNETKYRPLYIIDEHNNEKIADGGRL"
     gene            16094..>16297
                     /locus_tag="PKK75_03460"
     CDS             16094..>16297
                     /locus_tag="PKK75_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNZ82684.1"
                     /translation="MKNIVSKELAAYFIAGVLTTLVNFIVYYAMLFIGIEYKISNTAA
                     FIISVIFAFIVNKKYVFLSDKSYI"
     assembly_gap    16298..16397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16398..17656)
                     /locus_tag="PKK75_03465"
     CDS             complement(<16398..17656)
                     /locus_tag="PKK75_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ82685.1"
                     /translation="MLSFISLFNIIILIIFTGFYSYQIFYLFVALFKKGKNLKASKNH
                     RYAVVIAARNESAVIVQLIKSIKRQNYPGELLDIYVAADNCTDNTAEVARSAGAIVFE
                     RFNKQFVGKGYALDYAFKNIIYKKTCYDAYFVFDADNLLDENYISEMNKVFDNGYKIV
                     TSYRNSKNYDTNWLTAGYSLWFLREAKYLNNSRMILNTGCAISGTGFMVSDDIIRKNN
                     GWKHHLLTEDIEFTIDNALQGVKIGYCGKAVLYDEQPYLFKQSWNQRLRWAKGFYQVF
                     AKYGRDLVITIFKKRSFCCYDMFVTIMPALFLTLTSVGVNLTVFFVGLLNIETYPELM
                     GETIGAIFLSMLNSYIVLFLMGFITTVTEWKNINTSFMKKLKYIFTFPVFIITYVPIS
                     IVALFKFKKIEWQPITHSIVKTIEEMQ"
     gene            17915..19174
                     /locus_tag="PKK75_03470"
     CDS             17915..19174
                     /locus_tag="PKK75_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ82686.1"
                     /translation="MDDDGISQVAVLIIVLLTSAYFSAAETAFLNYNKSRMRNMEANN
                     KKAALVLNLEDNYDNLMSTILFFRSVLNVTAASLIIFIFSKLFVNKGILISTVIAIFI
                     IMTAGELIPRGLANMAPDKTAMSCAPSMRLFMRICSPFNFILATLKEMIYRVFKAEEQ
                     PSVYEDDLRTVLDEVENEGVIDKSESDLIRSAIEFNETVAEDIYTPRIDIVGIEENES
                     LESIKEKFILSGYSRLPVFSGGMDNIIGVLHEKDYYQALHRKEKDINKMVSKTIYVTP
                     NKKISELLKELQISKAHMAIVIDEYGGTEGLITMEDIIEELVGEIWDEHDEVIEWFKK
                     IGEDKYLVSCNADIDDMLDLFGIEVDDELDITTVNGFVTMLFEGIPEEGGRIVYKDLD
                     ITVTKAEAKRVIEIQVERIKTGRILTV"
BASE COUNT         6550 a         2924 c         4048 g         5162 t
ORIGIN      
        1 ttgaattatg aaggcaaggg ctatgcaaag ttaaaagaag atgttgcaga ggttgttgtt
       61 gaagaactta aacctataca ggaaagaata gattatttat tgaagaacaa ggattatctt
      121 gaagagatat acagcatcgg tgcgcaaaag gcagaggttg cagcccgcaa gaccttgaga
      181 aaggttcaaa agaaagttgg attcttgcca agatagaata atataatttg gaggaaaaat
      241 gctggttata aagaatggtt atataaaaac catggatgga gaagatattg aaaaaggtca
      301 aattattata gaaaacggaa aaatcaaagc agtaggaaga aatctggata tacctgaaga
      361 tgctgaaata tttgatgccg aagggctgct ggtaacaccg ggattagttg aagcgcactg
      421 ccaccttggt atgtttgaag aaggaatagg ttttgaaggt gatgatggca atgaatcagt
      481 ggaaccaatc acacctcaat taagagctat tgacggaata aatcccatgg accagggatt
      541 cgaggatgca agagagggtg gagtaacaac agctgttacg ggaccgggaa gtgcaaatgt
      601 aatcggcggt acttttgcag caattaaaac ctacggaaag aaaattgatg atatggttat
      661 taaggagccc gttgccatga aggtagcttt tggagaaaat cccaaaagag tatatgatga
      721 gcagcataaa tctcctgtta ccaggatggc tgttgctgca cttttgaggg aaacactttt
      781 tgaagctaaa aaatatatgg aagatttgga agcttccaag gaagatcctg accaaaaacc
      841 ggattttgac attaaacttc attcaatgat gccggtgcta aaaaaggaaa taccattaaa
      901 ggcacatgtt cacagagctg atgatgtttt tacagcgctt aggattgcga aagaatttga
      961 tttggatata accttggaac actgtaccga gggacacctg attgtggaag aattaaaggc
     1021 tgccggcaaa ccttgcttga taggaccaac cttcggacca agaataaagg ttgaaatgaa
     1081 aaataaaagc tttgaaacac ctaaaatgct tgcagatgca ggaatcaaga ctgccataat
     1141 tacagatgca aatgtcatcc ctatacagca cctcacaatg tgnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nntataaaaa aatcttgcat tatgcatgta aaaagaataa
     1321 actaagcgat ggaaatagaa tggagggggg acacatctct aattgagaat aagtctttgg
     1381 agtaagccat aagagaaatg tccccgtaat attatgaaga aaaaaattat aaatatagca
     1441 gtgattgcgc atgttgacgc aggaaagtct acattagtgg atgctttttt gcagcagagc
     1501 ggtgttttca gagaaaatca ggcagtaata gaacaggtaa tggattcaaa tgccctggag
     1561 agagaaagag gaataaccat atattccaaa aactgttcag tgatgcataa tggtataaaa
     1621 ataaatattg tagatacacc gggacatgct gatttttcat ccgaggttga aagaataatg
     1681 aagactgtgg atacggttat tctattggta gattccattg aaggacctat gccccagact
     1741 cgttttgtgc tgcataaatc tcttgaaata ggactgaggc ctattttatt aattaataaa
     1801 atagacaaaa agaaccacag ggcacaagag gttgttgata tggtctttga tttatttgtt
     1861 gaacttaatg caaatgatga gcagcttgat tttcccatcc tatacggcat tgcaaaacaa
     1921 ggaatagtcc aatatgaatt ggatgaggaa agcgacagta taattccctt gtttgataca
     1981 attattaaac atgttcagcc ttatcccgac agggacgaag aaaatttaca gatgcaggta
     2041 tcaactcttg cctatgatga ttatataggg aggctgggca taggaagaat tactgcagga
     2101 acaatcaggg aaggacagat ggtttctgtg gcaaagcatg acggcagtgt tgagaagcaa
     2161 aggatatcca atatttttat atatgaaggt ttaagacgca ccctggtaaa cgaagcattc
     2221 agcggagata tagtagtagt ttccggtatt tccaacatat ccataggaga caccatttgc
     2281 agcgaaaata atgtaattcc gatggaaaca ataaaaatag aagagccgac aatgtccatg
     2341 aattttttaa tcaatacttc gccctttgca ggcttgtcag gaaaatatgt tacaacaaga
     2401 catttaaagt caaggcttga aaaggaactt gaggttaatg taggacttag ggttgaagct
     2461 ttggaaggtg cccttgacgg ctataaggtg tccggaagag gtgaacttca cctgtccatt
     2521 ctcctggaga acatgagaag ggaaggctat gaggtttccg tatcaagacc cgaagtaatt
     2581 cttaagagga tagatggcct tgttatggaa ccggttgaaa gagttgtcgt acatgttccg
     2641 gaaatttatt cagggtctgt aatatcaaag cttaacttac gaaagggcat gatggtttca
     2701 atgatgcctg acggaaatta tgtgaaaatc gagtatatgg ttcctaccag aggactattg
     2761 ggttaccgga cggaattgat aaatgattcc agaggcgagg gaacaatggt aagaatattt
     2821 gaccattatg aaaaatttaa aggtgaaata ccgcaaagaa ccaacggtgt tttaatatcc
     2881 aacgaccagg gggaagcaat tgcatattca ttggataacc tggaaagcag aggaatcctg
     2941 tttattaacc ccggtgaaaa tgtatatgaa gggatgatta taggcatgaa ttcccgtgga
     3001 gaagatatta ccgttaatcc gtgcaaagcg aaaagacaat ccaatatccg ctcatcagca
     3061 tctgatgatg ccataaaatt atcacctccg agaataatga ctttggaagc agcgcttgag
     3121 tttataaatg atgatgagct ggtggaaatt acaccggatg ccataagact tagaaaaagg
     3181 tatctgactg aaatggaacg aagaaaacag ggaaataaga agccctaacc ccattnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatcat tgtaaaaact gaattattat
     3361 gatacaatta aataaataat tggtggaggg tgctatgaag gcaaaggaat gtttgttgtt
     3421 gatgcaaaaa attttgcagc tggtagatga aggcgttcat gtacttgata aggacggcaa
     3481 tacaatcata tataatgaat ccatgtctgc tttggaaaaa atgaaatcct gtgacgttat
     3541 gaagaaaccg tttactgagg tttttaaaga attgaacagc gaaaacagca ctcttctcaa
     3601 agccttgcat tcaaaaataa taacaaagaa tttaaaacaa acatatttaa acaaggacgg
     3661 aaaagaaatt accacaatta atacaactat acctgtcatt atggatgacg aggttatagc
     3721 tgcagtggaa gttgctaaaa acataaccaa aattgaggaa atgtccaata caatattaaa
     3781 gcttcatgat gaaatagaaa aaccggataa gataaaaaat aagaaagtaa aaaaatacaa
     3841 ttttgaaaat atagttggac aaagcgataa tttcttggat gttatagaca aggcaaaaaa
     3901 atcttccaaa aatactgcat cagtctttat ttacggtgag accggaacag gcaaggagct
     3961 gattgctcaa agcatacatt atgcaagctc aagaaaggac aagcctttca aagctcaaaa
     4021 ttgcgccgct cttccggaat cattgctgga aggtctgctg tttggcacat caaagggtgg
     4081 gttcaccgga gcagttgacc gtgccggttt atttgaacag gcaaatggag gaacacttct
     4141 tttggatgaa ataaattcaa tgccatatga actccagccc aagcttttaa gagtgcttca
     4201 ggaaaattat ataagacgcg tgggcgggtc aaatgatatt cccatcgatg taagaattat
     4261 aaccacatgc aatgagcctc ccgaaaaaat attaagggaa ggcaagctga ggaaggactt
     4321 attttacagg ctaaatgtta ttccattaaa tgtaccgcct ttaagggaaa gaatcaatga
     4381 tatcattctg cttgccaaaa tgtttacgga caaacacaat ataaggttta acaaacatgt
     4441 gacggatttt tctgatgaag tgtatgacta cctcttatca tacgattatc cgggtaatgc
     4501 aagagaactt gagaatatga ttatgtcagc tgtaagcatg tgtgaggatg atgaacatat
     4561 tttggacata ggacactttt ccattgtgca ctcaaaggat cataaaatta ataattatga
     4621 cagagtcgaa agtttaggca tggataagta tttaagcgac atagaaaaaa agattatcga
     4681 aaaagcactt ttagacaacg acaataacat tacacgtgca tcaggtgcat taagaataaa
     4741 acgtcaaact ttacagcata aaatcaagaa gtataagata aacttttaaa aaaagcaaaa
     4801 atatttgcag cttaatgcaa aataatttgc tttttttata ttttttgtaa aacataagca
     4861 cgaaaattct aaattcttga tttgaataaa attgcatctt ttaagaaaaa ataactgaaa
     4921 tgttgaaatt taaaggtttt gctatattaa aaacgtgcta tagaaattca tgtgaaatca
     4981 ttggcataat acttgcttag tatataagca agtaaaaaat tattaggagg ttttaacaaa
     5041 tgcaaaatgt aaaagtaatt atttggggac ttggagccat gggaagcggc atggcaaaga
     5101 tgcttttaag caaaaagggt gttgacatag ttggtgcagt tggaagaggc gctaaaatcg
     5161 ggaaaagcat gtatgaatta ataggagttg aaagaggaga caggcctgaa gtacttgttg
     5221 gagcaccgga agatgttata aaagaaaaag cggcagatgt agttttatgc tgtacagact
     5281 catatacaaa agttgcattc gaaagattaa aattctgtct ggagaaaaaa ataaatgtag
     5341 tttccagtgc agaagaaatg tctttcccaa tggctcaatc tcctgaagaa gctaaacaat
     5401 tagacgaaat agctaagaaa aacggtgttt caattattgg gacaggtatt aaccctggtt
     5461 taataatgga cttgttggtt gtagttatga caggatgctg tgaatcggtt gaccatatag
     5521 tttcaagaag agttaacagc ttatctccgt tcggaccaac tgttatgcat gaacaaggta
     5581 tcggtataac tgttgatgaa tttaaaaagg gtgttgaaac aggaacatta gccggtcacg
     5641 tgggatttga agaatcaata ggaatgatag cttcagctat tggctggaag gttgataaag
     5701 taactacatc catggatcct atagtaactg acgttgacag aaaatctcaa tatggatttg
     5761 ctaaagccgg caatgtagca ggtgttgcaa tgaagggttt tggatatatt gacggggaat
     5821 tgaaaattga aatggatcat ccgcaacaaa ttgaacctga gcaagttggt gttcaaacag
     5881 gtgattatgt aataattaaa ggaactccaa acatcaatat ggttaactca ccggaagttc
     5941 ccggaggaat cggcacaata gccatgtgtg ttaacactat tcctcatata atcaatgcta
     6001 agccgggatt acatacaatg cttactattc ctgtgccaag agcaataatg ggagatatga
     6061 gagatcttat agacccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaat
     6181 aaaaatattt taaggagtgt aacaatggtt aaaaaaggtg aatgggtttt aatacacaga
     6241 aatgttttag agccgtcgga aagagctccc caggttcctg atgacacaaa aaaggtaccc
     6301 ttggagttat ggactaaagg atttttgcag gaagatgcaa atattggaga tgaggtaaca
     6361 gttttaacaa gaaccaaaag aattgaaaag ggtacattgt tagaagcaaa tccttattac
     6421 aagcatgatt tcggaaaatt cgttcctgaa ttgctgacaa tcagcgatca agtaaaagaa
     6481 atcttatttg gaggtgctga taatgagtaa agatatgact tatgcagggg taatggcaag
     6541 aagaccggaa ataatgaaaa attcagcagg tcttgatttt tcaaaatttg aaagcggctc
     6601 aattgctttt gattatgaaa gaatgatgaa ggaagcagga tttaccattg aagaaatcca
     6661 aaaaattcag tctgaacacg gagtgggcaa tactcctata attgaactta aaaatttaac
     6721 agctctggca agaaaagttg ctcctgaagg aaaaggagca aggatattta taaaagacga
     6781 agcatcaaat gcatcaggaa gctttaaagc aagaagagca tctacagcag tatatcaggc
     6841 taagaagctt ggctacaaag gtgttgtaac ggcaacaagc ggaaactacg gtgcagcagt
     6901 tgcatcacag gcagcaatgt atggattaaa atgcatagtc gttcaggaat gctatgactc
     6961 aagaggagta ggtcagcctg aaatagttga aaaggccagg aagtgcgaag cattgggagc
     7021 cgaagttgtt caattgtcag taggtcctga actattctac atattccttt cgattctgga
     7081 agagacagga tacttcaatg cctctcttta tactccatat gcaattgccg gcgtagaaac
     7141 actgggttat gagattgcaa tgcagttcag agaaaaattc ggcaaggatc ctgatgttgt
     7201 aatagcaaca aatgcaggag gcggaaatct tacaggaaca gcaagaggac ttagaaaagc
     7261 aggcgctcag tctaaaatag tagcagccag cgttgatttg tccggacttc atatggcagc
     7321 agacacacag tttaacaaaa agtcatttac aacaggacat acgggattnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aaatcattga tcaggaatgc tttgaataat
     7501 tacaaggtaa caccaacaga agctgacata gaattcctgg cggcagaaac taattcggat
     7561 gtggaatttg taaaggaaac aatcgcatca ttgtaaaata tataataaat acaggcgtat
     7621 aggacataac gtcatgacat gaaatttcga atgttagtca aatacgccta tttcaaattt
     7681 aaagataaca ggaggatact tatgaaaaga gctgatgatt accagcaaag aagagctcac
     7741 ttagaaggac tttcagacga agaattatac gaaagatttt ggaaattagc aaatgaaatt
     7801 gttgaccctc ttttggaatt aggaagaaaa aacacaactc catctgttga aagaagcata
     7861 ctgcttcgaa tgggattctc aagcattgaa gttaagccta ttgtagaagg agttatgaac
     7921 aaaggtttga tgggaaaagg tgcaggaaat gttgtgtgga gactttcaaa aaaattaggc
     7981 tgctcagtgc gtgaggcagg attgctgctt gcagacggta aacactggga tgacgtagac
     8041 acattatttt aaggagggat gagaatgaaa cttgaacctg gcaaaaagtt agatgtaaaa
     8101 gaattattga aagacttgga taaatatgaa ccaagacgcc gtggatggca ttggagagaa
     8161 ggaagaaacg aaaagagaaa aataggtgat tttgaatatc acgaaacatc agagagccta
     8221 aaaaattcaa ttcctcttcc cggaagcagg ggctttggat atattgaccc tcagcctgac
     8281 tgtgtaataa ctactgaaat tgcttcagga agatttgaag atgatattag aagaatgcgt
     8341 atggctgcct ggcatggagc tgaccacctg atggttataa gaactgcagg ccagtcacat
     8401 attgactcac tgcttgaagg tacaaaccag ggtatcggag gtatcatggt tacaagaaaa
     8461 caagttagag catcgagaaa agcctgtgat ttaattgaag atgaaatagg ccgtccaatt
     8521 aacttccact catatatttc aggagttgca ggtcccgaca tagcagttat gtttgccgaa
     8581 gagggagtaa acggagctca ccaggaccct cagtacaatg tattgtacag aaacgtaaac
     8641 atggtaagaa gctttgttga tgcctgtgaa gccaagaaag tgatggcttg ggcagatatg
     8701 ctacaaatag acggtgccca caatgcaaat gccacagcaa tgaaagcatg gaaggttatg
     8761 ccggaattaa tggtgcagca tgctatcaat acggcatttt ctgtaaaaat aggtataaaa
     8821 cctgaaaata tagccttgtc aacagtacct ccaacagcac ctcctgcacc ctgcatgaga
     8881 ttagacctgc cctatgcagt agctcttcgt gatttgttca agggttataa gatgagggct
     8941 cagcaaaata caaagtacat ggaatcagac actcgtgaag cagctgtaac tcacacattg
     9001 aacatggtta tatcaagact tacaagcgct gacattcaat ctaccataac tccggacgaa
     9061 ggaagaaatg tgccctggca ctataacaac attgcaggaa caatgactgc aaaacaggcc
     9121 ctgacaggta tggacggctt cagagaaatg gttaagcttg acagggatgg tccattagga
     9181 gacaaagtcc gcgaactaaa ggaaagagca atacttttca tggaagaaat gattgaagta
     9241 ggcggatact tcaaggcagt tgaagaagga ttcttcatcg acagcggtga atatcctgaa
     9301 agaaacggag acggaatacc acgtcaaata gaaggcggaa tcggatacgg cttcatatac
     9361 gaaagagatg aggattattt tgcacccgta tccgtgcatt acggctataa caatattccg
     9421 gctgaatttg aaaaggcaag tgatgccata ggcggagata catttgagga tcggtctaaa
     9481 attcagtata ttgacgagct ggatgagcat gacaatgtaa atgtaagact taatgaagtt
     9541 gaaaaatact atgatacgaa tttagtaaaa ccggaagttg aatggagagc tgacggtacg
     9601 gttctgttga caatattcct gtctttggat gagagaacgg cagaatttgc tgcaattaaa
     9661 tgcggtgaaa agatgggtct ggaagatgtg acggttgttc ataaacaatc catacatcca
     9721 tccgaaggaa cttactgtga ggtaaagggc aaggtaaact ttgacatcga tattaacgaa
     9781 ctggtaattc ctgaaaaacc cgatattttg tccgaggcag aaataaggga agacattcaa
     9841 aataaaccta tgtttgttgt tgctgcaaca gttggagagg atgagcactc agttggatta
     9901 aaagaaacac tggatataaa acacggtgga attgaaggat tcggtataaa atatgaatac
     9961 ctgggaactt catgccctgt tgaaaagctg gtggatgcag caatagaaac taatgctgat
    10021 gccattttat taagcacaat aatcactcat gatgatgttc atattaaaaa catgaaaaaa
    10081 gttcatgatt tagcaataga aaagggtatc agggataaag tgatgattat atgcggaggc
    10141 actcaggtta caaatgaaac agccatggaa gcaggacttg atgcaggttt cggcagagga
    10201 acccacggga tagatgttgc tagcttcctg gtaaagagaa gaagggaaaa gaacgcataa
    10261 tgaaagtgaa tgtattggta gccgaaatcg gtagcaccac tacagtagtt aatgcattca
    10321 atggaatagg taccggtaac cctgcctttg cagggcaggg gcaggcacct acaaccgtat
    10381 tggaaggtga cgtaacaatc gggcttaagg gcgctattga ggacttaaaa aaaaacctgc
    10441 aggcggatac attgacatgg gatgatatgt tagctacaag cagtgctgca ggcggtttga
    10501 aaatgaccgt tcacggactt gtatacgata tgaccgttcg ggcagccaag gaagctgcct
    10561 tgggcgccgg aggaatcatc aagatgatta cttcaggcag gctcagaaaa acagatttga
    10621 aaaagataaa ggaaattgct cctaatatta tccttgttgc aggcggcgtt gattacggag
    10681 aaagagatac ggctctttac aacatggaaa tgattttatc catgggattg aaaataccgg
    10741 taatttatgc agggaatatc gaaaaccagg aagaagtccg cttgatgtgc gaagaagctg
    10801 acaaccggct ttatattgtt gaaaatgtat atccaaaaat tgacaccttg gttgtggagc
    10861 ctacaagaaa aataattcag gacgcctttg aggagcatat tatccatgca cccggtatga
    10921 ccaaggtaag agaatgggta aaaggtccta taattcctac tcccggtgca gtaatggagg
    10981 cagccagggt attaaaggaa gaaataggag acctgatagt atttgatgtg ggcggagcaa
    11041 caacagacct tcattcggta acagaaggtt caagcgaaat caattcaata ttgatcagtc
    11101 ctgaacctga agcaaaacgg actgttgagg gagacctggg tgtgtatgtt aatgtcagcc
    11161 acatagttga aaaaataggc atggataatt tgaaaaagga atttcccgat gcagaggagc
    11221 ttttaataaa ttacaagccc atacctataa ctcacaggga aaaactcttc gtggaaagac
    11281 ttaccaagga ggcggttctc acatcactgg aaagacacgc gggcaaacta aggtattttt
    11341 atactgcatc aggtaaaaaa accgtagctg aaggaaagga tttgaccgca attaagtaca
    11401 tcataggaac cggaggcacg ttgacaagat tatccggcaa caggaggatt ttggaaacag
    11461 ttaaaacata tgccaaggag catgaattgt atcctgcgga aaccgcaaag gttttaattg
    11521 atgaagacta tatattgtca tcgcttggag tattgtcaaa aagttacctg aatgacgctt
    11581 tgtacctcat taaaaaaagt ttaagaatgt aggtgattat atgtatccaa gattagcaat
    11641 tgatctaaaa aaggtaaaaa acaatcttga taagataacc caaatggtga aaggctccgg
    11701 aagctctctg atgattgtta caaagggata ctctgcagat atggaaatat acaaactatt
    11761 agacgagtca aatattgatt atctggcaga ttcacgtatt caaaatctta aaaaatatga
    11821 aggaaccaaa aaaggaagag ttttattgag gctgcccatg aattccgaaa ctgatgaagt
    11881 agtgaaatat gcagatgttt ctcttaattc ggaattaaaa actataaaaa acttaaatga
    11941 atcagcaaaa gttcaggata agattcataa aattctttta atgattgatt tgggagactt
    12001 aagagaagga atattcttca aaaatgaaga tgaaataaac tatactgtgg aagaaatctt
    12061 aaagcttgaa aacattgaaa ttttcggact gggcgtaaac cttacctgtt acggggcagt
    12121 aatacccaaa aaagacaatc tttcggttct tgttgatata gcacggaaaa ttgaaaagaa
    12181 atacgatata aagctgcaaa tgatatctgg aggaaattca agctctattt atttaatagg
    12241 aaaagatgaa ctgccggaag gaatcaataa tttaagagtg ggcgaagcat ttcttttggg
    12301 cggtgaaact gcttacggtc agaagcttga aggtttttac gacgatgctt tcacattgga
    12361 agcagaaata attgaattaa aggaaaaaca atcagtcccc atcggggaaa caggcgtgga
    12421 tgcttttggg aacaaacctg tttacgagga cagaggaata attagaagag ccataatagc
    12481 agtgggaagg caggatgttg atccggatgc ccttagtcct atggatgaaa aaatagatat
    12541 actgggagca agctcagacc atttgattct tgacgtgtca aagtcggaca atgaatacca
    12601 tgtagggggc atagtcaatt tcaaactcag ctactcaagc ctgctcaggg caacgacaag
    12661 cggctacgta gaaagagttt acaggtaaag ggaacaacaa aagagaccga cccggtaagg
    12721 gtcacatagg acaacaaata ttgggggcat acatgctccc tttttgttat tccggttgaa
    12781 ttgaataagg tttaccgcta tatcaaagca gatttctaaa tgttctatac cctgcttttc
    12841 aaatacccaa tttcatattt acagaaacat ttttctgtgc cataatatta atatcattga
    12901 aattggagtt aaaatgaaga atataaaaag gatggggatt tttttactat tatttgcctg
    12961 cactttagtt ttgctgttaa aagagccttt cgtgggaatt gccgacaaca gcgattacta
    13021 cagggtaatt cagcccttgg gatttcagcc tgaaataagc aacagatatt tttatgcata
    13081 taatttttat acagttaatg atatgtcaag tgaggatata aatggctccc tgtcaaatat
    13141 tatcagcccc aaggttgaaa atgacaatga atatttttca acccagttta ttttcattaa
    13201 aatttcaatg gttataaatt acttattaaa aataattctg gggaaatcgc cggaaatatt
    13261 caatataaag attttgggca ttttgtatgg gggaatttat tcttacggac tgtatttatt
    13321 tcttacaaac ctgaaattta aacgcaggtc catatatatc ctgtttttga ttatttccct
    13381 tgtaattctt tgcgatatgg gatatctttt atactttaat tcctttttcg gcgaagctgt
    13441 aataatttct tctttaatga tgacattagg cgctctttat gcgtttataa ggacagaaga
    13501 aagcggtaaa agcatttatt atggtatttt gttttatgtt ttcgccctgg cattaaccgg
    13561 agccaaggtt gcaaatactc caataggaat acttataggt ttattctcac ttacactttt
    13621 tattataaaa aaagaccggc ttaacaggac tttaattaca gcgggttcta tattgattat
    13681 ttgtttttcg gttttttatt atgccaatgc accccggtgg atgagccagg ttaacaatta
    13741 tcaaagtatt ttttacggta ttacaaaaga ttcaaaggaa ccggaaaagg atttggagaa
    13801 gctttcgata cctttaaaat atctgcctct gacaaacacc cacggttttt tggaccatgg
    13861 agactttgat atttacagcg atgagtttaa agaggaagta tatgataatg catcctttgt
    13921 ggacatatta aaattttatc tgttaaaccc atcaagagca atggaaaagc ttaagctgtc
    13981 tgctgacagc tcggtaataa taagaccttc atatttagga aactattcaa aggaggatat
    14041 gccggaaagg cttgaattta cacaaaggtt ttctttgtgg tccaatatca ggaaaaatac
    14101 tctgggctat gcctttaata taatagctgt tttttcagta ttattcttta ttataaatat
    14161 atacgaaatt attaacagca taaaccgccg ggacaatgaa aagattgttt tgtcttttgc
    14221 ggcccttttg ctgtttttaa caacaatcag ccagtttgtc ctgcctgtta taggaaacgg
    14281 tgaagctgat ttgcaaaagc atatgctctt attcaacctt tgctttgact taatggtgct
    14341 tgcagggtta aactggctaa taaacaatta tagcttaaaa atggttttaa agatagtctt
    14401 gacagcttcg gttttgttaa cagcaacaat acttattcag ccggcaaacg aaaaagtgga
    14461 ggaaacaggg ccattaagaa caggacaata tgtttatttt ggaacctata agaatgaacc
    14521 cctcaagtgg gttgtgctta atagcgacga aaacggcttt cttttatggt gtgataagcc
    14581 ggttgaatat atggagtttg ataacaggga tgaaacatct acggaaaatg tttacggctc
    14641 caacgactgg atagagtcgg atataagaaa atggcttaat tccgaattca agaataattt
    14701 taaggaagaa gataaacttt ttataaatga tgtcagactt aaaaatatat tgagctataa
    14761 taatattgac caaagcatag gaggcaataa gcctttttat tggaattcaa ttacatcata
    14821 tgtcagtcag aactataaca ctgatgctta ttacaattat tccgcagaag gagtttttct
    14881 tcttgatgcg tatcagctgg agaagttcgt ttatgaaaat aatattgata taaaaaagga
    14941 cgggagatac tggcttagaa cgccctacta cagctccgca agcatggtaa ggatagttga
    15001 cagggacgga tttgtctatc acaaggatgc aaatgttaag gcaggtgtga ttcctgcagt
    15061 ctacatagat gataatataa gagttatgca gggtgacgga acttattcaa gtccgtttac
    15121 catagaatag ggaggaaact atgaaaaagg taagcttatt gataccggca tacaatgaag
    15181 aggaaactat acccttgtta tacgatgaat taaacaaggt actggaaaca attcccggat
    15241 atgaatttga aatacttttt gtaaatgacg gcagcagcga taacacatta aatattctca
    15301 gaaatatgca gcaaagagac acaaggataa actacataag cttttcaaga aatttcggta
    15361 aagaaactgc aatggcagca ggatttgatt atgtaaccgg tgatgcagcg gttataatgg
    15421 atgctgattt gcaggatccg ccggagctta tcggggaaat gatcaaatat tgggaagaag
    15481 gctatgatga tgtatatgca aaaagaagaa gcagggaagg ggaaacatgg ctaaaaaaat
    15541 ttacttccgc agccttttac aagcttcttc agaaaatgac aaaaatacct attcaagaag
    15601 atacaggtga tttcaggctt ttggacagaa gagcaattga atccttaaaa aaactccggg
    15661 aaaaacaaag atataccaag ggaatgttca gctggatagg ctttaacaaa aaggaaatat
    15721 tatttgacag aaaaccaagg gcagcaggga agacaaagtg gaattacctg aagcttttca
    15781 atctggcatt ggagggagtt acatcattta ccacatctcc tttgagaata tcaaccataa
    15841 taggcattct ggtatcaatt ttcagcatga tatacatggt tattgttctt attaagagct
    15901 taatctggaa agacccggtt caaggatatc cttcaatgat ggttacaatt ttgtttttgg
    15961 ggggcgtaca gctggtgagc ctggggatca taggagaata tgtgggaaga attttcaatg
    16021 aaacaaaata tcggcctctt tatataatag atgaacacaa caacgaaaaa attgcagatg
    16081 gcggaagatt gtaatgaaga atatagtttc aaaggaattg gctgcttatt tcatagcggg
    16141 ggtgcttaca acacttgtaa atttcattgt ttattatgca atgcttttta taggcataga
    16201 gtacaaaata tccaatactg ccgcctttat tatttcggtt atttttgcat tcatagtaaa
    16261 caaaaaatac gtatttttaa gtgacaaatc atatattnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnntgc tgcatttctt cgattgtttt gacaatgctg tgagttatag
    16441 gctgccattc aattttctta aacttaaaga gtgcaactat tgaaataggc acataggtaa
    16501 taataaatac ggggaatgta aatatatatt ttaatttttt cataaatgat gtatttatat
    16561 tcttccattc ggtaacagta gttataaacc ccattaaaaa cagtactatg taggaattta
    16621 acattgacaa aaatattgca cctatcgttt ctcccataag ttcaggataa gtttctatat
    16681 ttaacaaacc tacaaaaaaa acagtcaaat ttacacctac gctagtcaat gtaaggaata
    16741 acgcaggcat tattgtaaca aacatgtcgt agcagcaaaa gcttcttttt ttaaatatgg
    16801 ttattaccag gtccctgcca tatttggcaa atacctgata aaacccctta gcccatctta
    16861 atctttggtt ccatgattgt ttaaagagat aaggctgctc gtcataaaga acagcttttc
    16921 cacaatagcc gatttttacc ccctggaggg cattgtcaat tgtaaattca atatcctctg
    16981 taagtaggtg gtgtttccag ccgttgtttt ttcggattat atcatcgctt accataaaac
    17041 ctgttcctga aattgcacac ccggtattta gaatcattct tgaattgtta agatatttgg
    17101 cttctcttaa aaaccacagg gaatatcctg cagtcagcca gtttgtatca tagttcttgg
    17161 aatttctata actggtgact attttatatc cgttgtcaaa caccttgttc atttcactta
    17221 tatagttttc atccagcaga ttatctgcat cgaaaacaaa gtatgcatca tagcaggttt
    17281 ttttgtatat tatattttta aatgcataat caagagcata tccctttccc acaaactgct
    17341 tgttgaatct ttcaaatact atcgcacctg cactccttgc aacttctgcc gtattgtccg
    17401 tgcagttgtc tgctgcaaca tatatatcca ggagctcacc gggatagttc tgccgtttaa
    17461 tactctttat caactgtaca ataacagcac tttcatttct tgcagcaata acgaccgcat
    17521 atctatgatt ttttgaagct tttaaatttt tacctttttt aaacaatgct acaaacaagt
    17581 agaatatttg ataggaataa aaacctgtaa atattattaa tataattata ttgaataaac
    17641 ttataaacga taacattgat acctccatta gctgtcagtg tgaatataag catttacata
    17701 aaagaaatca attaagaatt tcttaattat attttaaaat ttaatgatat tattattatc
    17761 acatcaagct tttttatgct tatatcgtct gccttaattt tccttaaatt tgagctgtga
    17821 aattttaaag aggcttgaat ttaatgaatt gacacttttg ttttaatcaa ttatactata
    17881 tacaaataaa ttgaagtggg agtgttaaac aaaaatggac gatgacggta taagtcaggt
    17941 cgcagtgtta ataattgttt tattgacatc tgcatatttt tcagcagcag aaactgcctt
    18001 tcttaattac aacaagtcaa gaatgagaaa catggaagca aataataaaa aagcagcact
    18061 ggttttgaat ctggaagata attatgacaa tcttatgtca actatattgt tttttagaag
    18121 tgttttaaat gttacagctg catctttgat tatttttatt ttttccaaat tgtttgttaa
    18181 caagggaatt ctaatttcaa cagttatcgc aatttttatt attatgacag caggggagct
    18241 tattccaaga ggtttggcaa atatggcccc ggataaaaca gcaatgtctt gtgcaccttc
    18301 aatgagatta tttatgagga tatgttcacc ttttaatttt attcttgcca cgctgaagga
    18361 aatgatttac agagttttta aagctgaaga acagccttct gtttatgaag atgatttgag
    18421 aaccgtattg gatgaagtag aaaatgaagg ggtaattgat aaaagcgaaa gtgatttgat
    18481 tcgttctgca attgagttta atgaaacagt agctgaggat atctatactc caagaattga
    18541 cattgtggga atagaagaaa atgaaagttt agagtcaata aaggaaaagt tcatattaag
    18601 cggatattca aggctgccgg ttttttcggg aggcatggac aatataatcg gagtactcca
    18661 tgaaaaggat tattaccagg ctttacacag gaaagaaaaa gatattaata aaatggtttc
    18721 aaaaaccatc tacgttacac caaataaaaa gatatccgag cttttgaaag aactgcagat
    18781 ctccaaagcc catatggcca ttgtcataga tgaatacggc ggaactgaag gactgattac
    18841 aatggaggat attattgaag agctggtggg cgaaatttgg gatgagcatg acgaagtaat
    18901 tgaatggttt aagaaaatcg gagaagacaa atacctggtg tcatgcaatg cagatattga
    18961 tgacatgctt gacctctttg ggattgaggt tgacgatgag cttgatatta ctactgttaa
    19021 cggatttgta acaatgttgt ttgaagggat tcccgaagag ggcggaagga ttgtttacaa
    19081 ggacttggat ataaccgtca caaaggcaga agcaaaaagg gtaatcgaaa tacaggttga
    19141 aaggataaaa acaggaagga tcctcacggt ataaatatat atga
//
LOCUS       DAOPUT010000021        18868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_2965_length_18868_cov_20.325520, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000021 DAOPUT010000000
VERSION     DAOPUT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 18868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18868
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2965_length_18868_cov_20.325520"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..4420
                     /locus_tag="PKK75_03475"
     CDS             398..4420
                     /locus_tag="PKK75_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNZ82687.1"
                     /translation="MNEILLNYIVQAVLGGASGYITNDYAINMLFKEYTPLKLGGVIK
                     KTRVEFIENLSSMVENDIINKEKLHEIFNSRDFRDKFEKLTCDFYENCLQETIGQVKF
                     SDIDGFEPTMKKTDNYVQKIFSENIDGLIELIIDNFDAGCFLTKIQSDKIAESLYGTI
                     KDVIKNTDVLENSLKYLYKNNKELKLSDVLGSNIRTIDVSINKLAEIAANKVPSLESI
                     AGFNSAVNEALDIFYDKHIKDVIKFDKKLKSSFISYITDKDNVHKVCRSLVSYGKTID
                     KSLYSILDPAFESSLKAYIQENLPYVTEQLVSYVQKNSLLIDGIIEDSIDEVIGESQG
                     LRSKLLYSVKNAYFNNLSKKHSIVQRIISFIRQSTEPERLSSHISKRIINRLDTVTIR
                     DIFIGAENSFDLDKAYEIAAEFLSKSAGGILENAGNYVLQLRVKEILPRFDFSAEKLL
                     SSGPVLGFLKSKSQDLVKAILCKELSELASEDKLGHYAVKAAQYIKKEYCNNKNSIAD
                     YISEKIKNFEINKNNLKSKELMDLVHKESYDKYKEEASKLKDVNLSFALDKLNSIENL
                     SKNSSETLRKYIINNTDSILKGSIKSIVTDNLNKLSDDELVNFANDFIGRELKPIMLF
                     GGVLGIAAGLILAAFQNTPLVPSEINILNMAVYAFVGFITNVIAINMIFKPYRKIKIL
                     SKLPFFSNFSLGYIVKNQKNFAESTAHYIDTGLLSKKSINEMFEKHKDSIKQSFVKNI
                     GENDYAVLSSLLVKNKEGIVKGTLSFLKKNILKNLSAFSNYLYGEVSRIKLSKVLTDT
                     NIDSLSSYASENIKTDNIKNRIYSLIKSDKKFNTVFSTEFFIEILCNGFKKLYDKTID
                     FLNPATINNEILKHNNKYNKYSDNIIEEVFTLNDIAGFSQKVNTAVFSENFKFHVSSA
                     IIILFNKLFDRDKSFGELFEGRLKVYIDKNLTQILKTVTQKIKDNLSESKTIVSTSLR
                     AEFKNNLGFIQSGVYALMDGDEIIDSLVNKIMTEKLPLFMDVKKDEINKIMAGLIIDR
                     FYMTPVEALYVNLNKMQINEVVENYLSANKEKLESKIKNLTEELYNKIRYKNVRSILE
                     FFSLGDLEGFIKSYESEINAFAAAIHPYLTDNKNGIDNDISDYLVSSAEEFMDLKVSD
                     IFIYESKDDIDKIIDNITKVIKKNDAVRKTVKSLEEEYIKYFSNISLDYYIDKDEFFK
                     STEMFVQSLLMNDELKTRLKNILFAVFDDASEHNFSFIDSKTKEYAVNAFVDASIESL
                     RRNLDDMLKSVEFEKIAQEEIEKMEPDKIHQMFNSFGKKYFRKLMLYGFGGFVFGINM
                     YVGFALTGLKIFSDTIKKDR"
     assembly_gap    4491..4590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4591..5688)
                     /locus_tag="PKK75_03480"
     CDS             complement(<4591..5688)
                     /locus_tag="PKK75_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ82688.1"
                     /translation="MRRKISLLIVLMLVLSTFTNVSAIEEAVEGEKVLQAAEAAETEE
                     TVEIEPLLLSLEDAVRIARENSREMWKIDDGIAQVKDARKDAKTAKQAAEYIMSLPLS
                     KIEEELGPMFDVTSDQVERIMAKNGYYITFADTQQAQLEKNREMLLAGIEIETTSLYY
                     NVLLAEKSIAINKVNLNKAEEQLRVVNLKFNNGSATKAGVLNAEMAVQKAKTDLDSAE
                     DDYNIAVLNLLNKLELGFDTKPVLTDTEFSYVPSAEINLDDAIEKAKAERPEILTAEN
                     SLELQKIETHAYTAYYTSNLRQHKAAEEKLKDAEINAEQSYKNVELDVRISYLNLIKA
                     ERALANMEKTLELAKEAARINQLLYDNGMAAN"
     gene            complement(5701..6288)
                     /locus_tag="PKK75_03485"
     CDS             complement(5701..6288)
                     /locus_tag="PKK75_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ82689.1"
                     /translation="MEKNQKRQDILQAAMALFMDKGFQNTKMEDIAIDAGVGKGTLYE
                     YFENKQDIFDEACIEYVNVIIDNIRYVSNMDDTFNNKLLILFNGKLKMESDFEDMSID
                     NILSSKNIISEETIKKIILKISEMYGLICDIIDQGKEEGVVKKSIKSEITACMIMGTM
                     GEYLRLISHKKENEIKDDDVIFDLLYNGFAVKKHQ"
     gene            complement(6307..>7932)
                     /locus_tag="PKK75_03490"
     CDS             complement(6307..>7932)
                     /locus_tag="PKK75_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ82690.1"
                     /translation="KLISVKNLESEEEQEKKQGPLVVSYKKVLSLSLRHRFITILSSI
                     ALFIISVMMVISVGAEFFPATDEGMINVSVSMPAGSETEELDALLKEIQDSIKEIPEI
                     EFTFTAAGGGGMYGIGGGSTGSMNVLLKDLSERDKSAKQISDEIRTLTKDIPGAEISV
                     AESTSMMMGTLTGAISISIKGDDMDTLKAIGDDFKNIIEKVEGTREVSTSYEDGIPQV
                     QIIADRGIASQYGLTTAQIGNSVINTLSGKNVTKFKVDGDEIDVVLKGDNVYGESLSY
                     LEMLPVETPAGSSVPLSEVAEIKVEKGPVSIYRENQVRVLTVSGSVVGRDVQSASVEI
                     EDLLKVYEMPYGYSYMYGGEIEQIQETFTDLFLVMLMAIALVYMIIAAQFESLIQPLS
                     IMFSVPLALSGGFIGLFIAGLPLNVIGIIGLIILVGVVVNNAIVLVDYINIRRRRGET
                     RDAAIMKAGPIRIRPIMMTALTTILGLVPMSLGIGEGAELIQSMGVVVIGGLSLSTVL
                     TLIIVPVMYTIFDDISESFKRRIKKDKITEQIQ"
     assembly_gap    7933..8032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8033..8632)
                     /locus_tag="PKK75_03495"
     CDS             complement(<8033..8632)
                     /locus_tag="PKK75_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ82691.1"
                     /translation="MLAKFSVKKPVTITMLILIVIVIGVVSFSKLQIDLLPQMDLPYI
                     MVQTNYQGAGPEEIESIISKPMEQTLSTVENIEAVISISSEGSSLLLMQFAYNTDMDE
                     VMLQIRERVDVIKGFLPEGTSSPLALKLDPNALPIVQLAVSSKGDLHTTQKIAEDVIS
                     PRFERIEGTASASVTGGLDQEVEVMLREEAIKGYNLSSSY"
     gene            complement(8635..9741)
                     /locus_tag="PKK75_03500"
     CDS             complement(8635..9741)
                     /locus_tag="PKK75_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ82692.1"
                     /translation="MKRLLTLLLIIVFVLAGCTAGQETNADKEEAYTPVEVETLKPME
                     LYIENIMTAKVFADKDVFVIPVMAGKVEKINVNVGDRVEKDDVLFVMDKDDIQKQVNQ
                     AYTAYEAAKAGFEVSSAQIQSARDNLERIEKLYKEGAVPETQYEQAKLAASDEALEAA
                     RMGLEQAKVAYSNSASMLKNAEVKAPIPGVVSSVNIVEGEYATSANPPVTIVDSDSVT
                     IEFGIAASLVNKVKTGDGVLVEISSAGYKNEAFINSVSSSADIMTNLYTVSIVLENDG
                     TIKPGMFAKVYLKTDNIENALAVRTEAVKEREGKQFVFVAEGEAAVEKEVTTGLDTGT
                     YIEIKTGISQGDKVIVKGQDYISQGSKIKVVRGE"
     gene            10278..11222
                     /locus_tag="PKK75_03505"
     CDS             10278..11222
                     /locus_tag="PKK75_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNZ82693.1"
                     /translation="MNFLKIEDEMKYFQRIFMLRKEIENFLFKEGYMNIEPSIFEEYD
                     EFTSVNSRIDKKSTVKILESNSDILILSPDITTGIINKFMPRWDYDLMLKIFYYGKTY
                     KHNGNGIKEKREIGIEIIGDNSQNIDQNTLMTAERIISRYSDNYLLEIGNSKFLKGIM
                     LACSFNKEDYDNILDLLYLKNKQELDDYLSKLNYKEPICTLLNILDLEGTYEEITGKL
                     EGLYLNDMMKAGLQELKTVDDYMKNITDKIIYDLSMVSELSYYDGIIFRGYIHGSNTE
                     IIKGGRYDSFTEQFGVKIPAIGFTVELDELLKAIYKEG"
     gene            11225..11860
                     /gene="hisG"
                     /locus_tag="PKK75_03510"
     CDS             11225..11860
                     /gene="hisG"
                     /locus_tag="PKK75_03510"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNZ82694.1"
                     /translation="MSLTIAIPKGRLGNRTIEILKAAGLGSAIDEDSRKLVFNSENRS
                     IKFILLKNSDVITYVENGIADIGISGKDMIMESSGDFYELYKMNVGRCKMSVAGFKDK
                     KVFNDDTILKVATKYPETARKFFNSRGQKIKIIKLNGSIELAPILGLSDVIVDLVETG
                     NTLKANGLEVFEDMYDISAVIISNKISYKFKRKEIKEIIELIRREDNGQVN"
     gene            11844..13139
                     /gene="hisD"
                     /locus_tag="PKK75_03515"
     CDS             11844..13139
                     /gene="hisD"
                     /locus_tag="PKK75_03515"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNZ82695.1"
                     /translation="MVRLIENNIEEYLAEIQNRNQDDYREVYKAVDEILEDVRKNKDE
                     ALKKYTMEFDKVSLNNLEVSEDEIKEAYQTIDKELLETIKKAKENIYDYHKRQVRRSY
                     KYKPEGKDIILGQLINPIEKVGIYVPGGKAAYPSTVLMNAIPAKIAGVNEIIMITPPQ
                     KDGKLKDSILVAADMAGVDRIFKVGGAQGIGALAYGTETIPKVYKITGPGNIYVAAAK
                     RRISGFVGIDMIAGPSEILIIADKTASPAYIAADMISQAEHDEMAACILITNSYLLAD
                     KVKKELKNQSQLLERREIIEKSLNNYGAIIITDSMKHSIDLANEIGPEHLELLTTNPF
                     ENYKKIKNAGAIFLGGNSPEPVGDYFAGPNHTLPTNSTSKFSSGLSVDDFIKKTSLIY
                     YSRQALLENGGRIVRFAENEGLTGHANAIKIRMQGGEND"
     gene            13132..14157
                     /gene="hisC"
                     /locus_tag="PKK75_03520"
     CDS             13132..14157
                     /gene="hisC"
                     /locus_tag="PKK75_03520"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNZ82696.1"
                     /translation="MIKLKQSAERIEAYFVNNVDCRVKLDANEGSNYLLKDGFKIDSF
                     KVNLYPDSDCSSLREKMAFYYGCKAENIMVGNGSSEIINTVINAYCEKADKVMSFVPS
                     FSMYKTYCDLCSAEYVGVDSEDDFSQDINKLIKEAKMLDPKIIIICNPNNPTGYVTPM
                     EDVIKVLENFKNSLVILDEAYADFSGNSALGLINDYENLIVMRTMSKAFGLAGLRVGC
                     LIAAEKTVKYIWKVKLPYNVNILSQYAAEKALSNSGRVVEYIESVKIRREELSSELKK
                     LDFLVYPSGANFLFVKPPIQNLFEKLMERGILIRKFNDTYYRISVGEKEENEILTEEL
                     IKIRREQ"
     gene            14154..14732
                     /gene="hisB"
                     /locus_tag="PKK75_03525"
     CDS             14154..14732
                     /gene="hisB"
                     /locus_tag="PKK75_03525"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNZ82697.1"
                     /translation="MRKAVITRNTSETKIQIELNLDESGAREIDTGVGFLDHMLTLFC
                     FHGKFDLNIKCKGDLNTDTHHTSEDVGIALGQAFTKALGDKRGIERFGAAYVPMDESL
                     ARVVLDLSGRPCLVYNLDFKKAQIGTMATEDFKEFFQGFANNCLCNLHIELLYGENDH
                     HKIEAVFKGFGRAMKKAVEVTSDSLQSTKGAL"
     gene            14732..15439
                     /gene="hisA"
                     /locus_tag="PKK75_03530"
     CDS             14732..15439
                     /gene="hisA"
                     /locus_tag="PKK75_03530"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNZ82698.1"
                     /translation="MILLPAIDIKDNKCVRLTQGKFDKLVVYSDDPGEMARKWESMGA
                     EYLHVVDLDGAKNEGFQNRRSIEKIAKSISIPIQTGGGIRSEERVKDLINIGVDRVIV
                     GTMAIENRELLTKLVKNYKDHIAVSIDALKSKVATRGWQRVENVDSIEMCKALESMGI
                     RTIVYTDISKDGMLSGPNFDIYRELSTKTIINIIASGGVTTPEDIKKLREMNLYGAII
                     GKALYDGRLDFKDALKI"
     gene            15457..16200
                     /gene="hisF"
                     /locus_tag="PKK75_03535"
     CDS             15457..16200
                     /gene="hisF"
                     /locus_tag="PKK75_03535"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNZ82699.1"
                     /translation="MAVRIIPCLDVINGRVVKGTKFKDIKDVDDPVNLGKYYSDSGAD
                     ELVFYDITASLEGRKTSLEFVKKVAAEINIPFCVGGGISSIEDFKHVLESGADKISIN
                     SAAVKNPYLIKEASGIYGSKCLVLALDVKRNEEGSWSVYVKGGKEKTSLDAVEWVKTG
                     VKLGAGEIVVNSIDEDGMKTGYDLELLKRVKAAVNVPVIASGGAGKYEHFLEAVKYAA
                     VDGVLAASVFHFGEIKIPELKKYLRENGL"
     gene            16214..16834
                     /gene="hisIE"
                     /locus_tag="PKK75_03540"
     CDS             16214..16834
                     /gene="hisIE"
                     /locus_tag="PKK75_03540"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNZ82700.1"
                     /translation="METQMLNGIKFDEKGLILAIAQDYITSEVLMAAYMNEKALEITL
                     KEKKVCYFSRSRNCLWRKGETSGHVQHLKGLYYDCDADAILLKVEQVGNACHTGAYSC
                     FFNKVYEEDIADKSIINKVYCQIQNRKDNPKEGSYTNYLFNEGLDKILKKIGEESSEV
                     LIAAKNKNKEELIYEISDLIYHSLVLMVSEGVTIDDLQKELTKRHR"
     gene            complement(17118..18563)
                     /gene="proS"
                     /locus_tag="PKK75_03545"
     CDS             complement(17118..18563)
                     /gene="proS"
                     /locus_tag="PKK75_03545"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNZ82701.1"
                     /translation="MAKKEKEFVKEITPMEEDFSRWYTDVIMKNELVDYSPVKGFMVI
                     RPYGYALWEKIQEYADEKFKATGHKNMYFPLLIPESLLTKEKEHVEGFAPEVAWVTHG
                     GSQELAERLVIRPTSETIICHMYAKWLNSYRDLPYLYNQWCSVVRWEKTTRPFLRTSE
                     FLWQEGHTLHETEEEAQAETLQMLDIYRQVAEDLMAIPVVVGQKSDKERFAGAHATYT
                     MEALMHDGQALQAGTSHNLGQHFTKAFEIKFQGRNGQEQYPYHTSWGISTRLIGGLIM
                     VHSDNRGLVLPPKMAPVQVVIIPIAQKKEGVLEKANELYEKLKAKGIRVELDDSTEYS
                     PGWKFNQYEMKGYPIRIEIGPRDIDNNQAVAVRRDKLEKETLSLDNIEDTIYNMLETM
                     QNDLFEKAKAHREAHTYIVKDYEQYKKDLPNRPGFAKMMWCGERECEDKLKEETGATI
                     RCIPFEQEDLGDKCHICGKPAKHMIYAARAY"
     misc_binding    complement(18599..18801)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         6094 a         3316 c         3530 g         5728 t
ORIGIN      
        1 ataataatca atataatttt acgatacccg gcaaggtatg tatgtagaca tcccggcagg
       61 ggaaaacgga caaactttca aaaaacagag gctctgtggg ggacagattt tgaaaaattg
      121 tccttgtagt agagatagtc actatggatg gaggaattcc tcgaataaaa atgtattttg
      181 cttatttata ttttaagtgc tataatccca taataagtat cacaggtatg tggcacacct
      241 gcaaaggaat agggatacaa attcaaaatc atgcgacttt atgggggcag tttttaagga
      301 atgacccgta cctggaataa tcccaaggtc aagagatgtc ccccgattat taatctatgc
      361 aatataggaa tgttcccaaa aagtaaaagg tgataatatg aatgaaatac tgctaaatta
      421 catagttcag gcagtcttag gcggcgcctc cggttatata acaaatgact atgccataaa
      481 catgcttttt aaggaataca ctcctttaaa attaggcggt gtcattaaga aaacaagggt
      541 agagtttata gaaaacttaa gctccatggt tgaaaatgac ataatcaaca aagaaaaact
      601 tcacgaaata tttaacagcc gggacttccg ggataaattc gaaaaactta cctgtgattt
      661 ctatgaaaac tgtctccaag agactatcgg tcaagtaaaa ttttcagata ttgacggatt
      721 tgaacctaca atgaagaaaa cagacaatta tgttcaaaaa attttcagtg aaaatattga
      781 tggcttaatt gaattaataa tcgataattt tgatgcgggc tgttttctta caaagataca
      841 atcagacaaa attgcagaat cattatacgg taccataaaa gatgttatta aaaatactga
      901 tgtattggaa aattccttaa aatatttata taaaaacaat aaggaattga agctttcaga
      961 tgttcttggc tcaaatattc ggacaattga cgtctcaata aataagcttg cagagattgc
     1021 agcaaataag gtcccctcct tagaatcaat tgcaggattt aatagcgctg taaatgaagc
     1081 tctggatatt ttttacgaca aacatattaa agatgttatt aaattcgata aaaaactaaa
     1141 aagttccttt atttcctaca ttacagacaa agataatgtt cataaggtct gccggtcatt
     1201 ggtttcttac gggaaaacta tagataaaag tttatattca attttagacc ctgcttttga
     1261 aagcagtctt aaggcatata tccaggaaaa tcttccatat gttactgaac aattagtcag
     1321 ctatgttcaa aagaacagct tgcttatcga cggaattatc gaagattcca tagatgaggt
     1381 aataggtgag tctcaaggac taaggtcaaa attgctgtat tcagttaaga atgcctattt
     1441 taacaattta agcaaaaaac acagcatagt tcaaaggatc atttcattta tcaggcagtc
     1501 tacggagccg gaaaggctta gcagccatat cagcaaaaga ataatcaacc gtctggatac
     1561 tgttaccata agggatattt tcataggagc agaaaacagc tttgatcttg ataaagctta
     1621 tgagattgct gcagaatttt taagcaaaag tgccggcggt atcttagaaa atgcagggaa
     1681 ttatgttctt caactgcgtg tcaaggaaat cttgcccagg tttgatttct cggcagaaaa
     1741 attattatca tccggtccgg tgctggggtt tttgaaaagt aaatcacaag accttgttaa
     1801 agccatatta tgcaaggaat taagcgaatt agccagtgaa gacaaacttg gtcattatgc
     1861 tgttaaagca gcgcaatata taaaaaaaga atactgcaac aataaaaact ccattgctga
     1921 ctatatatct gaaaagatta aaaattttga gataaacaag aataatttaa aaagcaagga
     1981 gctcatggac ctggttcaca aggaatccta cgataaatac aaggaggaag catcaaaact
     2041 aaaggatgtt aatttgtcct ttgctcttga taagttgaat tccatagaaa acttatcaaa
     2101 aaacagttca gaaacccttc gcaaatacat tataaacaat accgactcaa ttctaaaagg
     2161 ctccattaag agtattgtca cagataatct gaacaagcta agcgatgatg aacttgttaa
     2221 ttttgccaat gattttatag gcagggaatt aaaacccata atgcttttcg gcggagtatt
     2281 ggggatagcc gcaggcttga ttctggctgc atttcagaat actcccctgg ttccttctga
     2341 aataaatata ctcaatatgg ctgtctatgc atttgtaggt tttattacaa atgtaatagc
     2401 aataaatatg atattcaaac catacagaaa aattaaaatt ttatctaagc tacctttttt
     2461 cagcaatttt tcacttggct acatcgttaa aaaccagaaa aatttcgcag aaagtactgc
     2521 acattatatt gacaccggtc tcttaagcaa aaaaagcata aatgaaatgt ttgaaaagca
     2581 taaagatagt attaagcaaa gctttgtaaa aaacattggc gaaaatgact atgccgtatt
     2641 aagcagcctg cttgttaaaa acaaggaagg aatagtgaag gggaccttaa gttttcttaa
     2701 aaagaatatt ttgaaaaact taagtgcttt cagcaattat ttatatgggg aagtttctcg
     2761 gataaagctt tctaaggttc ttacagacac gaatattgac agtctaagta gctatgcaag
     2821 cgaaaatata aaaactgata atataaaaaa tagaatttat tcgcttataa agtctgataa
     2881 aaaatttaac accgtatttt caactgagtt tttcattgag attttatgta acggttttaa
     2941 gaaattatat gataaaacaa ttgatttttt gaaccctgcc actataaata atgaaattct
     3001 aaagcataat aacaaatata ataagtattc ggacaatata attgaagaag tatttacttt
     3061 aaatgatata gccggttttt cccaaaaggt taacacagct gttttttcgg aaaattttaa
     3121 gtttcatgtt tcttccgcta ttataatttt gtttaacaaa ttgtttgaca gggataaaag
     3181 ctttggagaa ttgttcgaag gcagattaaa agtatatatt gacaagaatc ttactcaaat
     3241 tctaaaaact gtgacacaaa aaatcaaaga taacctttca gaaagcaaaa caatagtatc
     3301 tacaagcctc cgggctgaat ttaagaataa tttaggtttt atccaaagcg gtgtgtacgc
     3361 cctcatggat ggtgatgaaa tcattgacag ccttgtaaat aaaataatga cagaaaagct
     3421 gcccctcttt atggatgtga agaaggatga aataaataaa attatggctg ggctgataat
     3481 tgacaggttt tatatgacac cggttgaagc tttgtatgta aacttaaaca aaatgcaaat
     3541 taatgaagtg gtagaaaatt atttaagtgc aaacaaagaa aagcttgaat caaaaataaa
     3601 aaatttaaca gaagagcttt acaataaaat aagatataaa aatgtcagaa gcattttgga
     3661 attcttcagc ctaggtgacc tggaaggttt tattaaatcc tatgaatctg aaattaatgc
     3721 atttgctgct gcaatccatc catatttaac agataataag aacggaattg ataatgatat
     3781 ttctgattat ttagtttctt ctgcagaaga atttatggac ttgaaggtaa gtgatatttt
     3841 tatttatgaa agcaaggatg atatagacaa aattattgat aacataacaa aggttattaa
     3901 gaaaaatgat gctgtaagga agactgtaaa aagcctggaa gaagaatata taaaatactt
     3961 tagtaatatc agtttggatt attacattga caaggatgaa tttttcaaat caacagaaat
     4021 gtttgtgcaa agcttattga tgaacgatga actaaaaaca aggttaaaaa atatcctttt
     4081 tgcagttttt gatgatgcct cagagcataa tttcagtttt attgattcga aaacaaagga
     4141 atatgctgta aatgcatttg ttgatgcaag tatcgaaagt cttagaagga acctggatga
     4201 tatgcttaag tccgttgaat ttgaaaaaat tgcacaggaa gaaatcgaaa aaatggagcc
     4261 tgataaaatt catcaaatgt ttaattcatt tgggaaaaag tatttcagga agctcatgct
     4321 ctacggtttc ggaggttttg tattcggtat aaacatgtat gtgggatttg cgcttacagg
     4381 attaaaaata ttttccgata caatcaaaaa ggaccgttag gcggtccttt gactgtgttt
     4441 gaagttattg atttacataa atttaaatat gtaaccctag tgataaaaat nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtttgcagcc attccgttat catacagaag
     4621 ctgatttatt cttgctgcct ccttagctaa ttcaagggtt ttttccatat tggcgagagc
     4681 tctttcagcc tttattaaat tcaagtatga tattcttacg tccagctcaa catttttata
     4741 agattgttct gcatttattt ccgcatcctt cagcttttcc tctgctgcct tatgctgtct
     4801 taaatttgat gtatagtatg cggtataggc atgggtttcg attttctgaa gctctaaact
     4861 gttttcagcc gtcagtattt caggtctttc cgcttttgct ttttctattg catcatccag
     4921 gttaatctct gctgaaggga catatgaaaa ctccgtatct gtcagcaccg gttttgtatc
     4981 aaagccaagc tccaatttgt tcaataaatt caaaactgca atattgtaat catcctctgc
     5041 agaatccagg tctgtcttgg ctttttgaac tgccatttct gcatttaaaa ctcctgcctt
     5101 tgtagctgag ccgttattga attttaaatt tacaaccctc aattgctctt ctgccttgtt
     5161 taaattaacc ttgttaattg caattgactt ctctgccaaa agcacattgt aatacagtga
     5221 tgttgtttct atttcaatac ctgccaaaag catttctctg tttttttcaa gctgtgcctg
     5281 ctgagtatcg gcaaaagtta tatagtagcc attttttgcc attattcttt caacctgatc
     5341 acttgttaca tcaaacattg gaccaagctc ttcttcgatt ttagataatg gcaaagacat
     5401 tatgtattct gctgcttgct tagctgtctt tgcatctttt cttgcatcct taacctgagc
     5461 tattccatca tctattttcc acatttctct gctgttttct cttgctattc tcacggcatc
     5521 ctccaatgac agcagaagag gttctatttc tacagtttct tctgtttctg cagcttctgc
     5581 agcttgcaaa actttttcac cttccacagc ttcttcaatt gccgatacat tagtaaatgt
     5641 gcttaatacc agcatcaata caattaataa agatatcttt cttctcataa tttcctcctc
     5701 ttattgatgt ttttttacgg caaatccgtt atacagcaaa tcaaaaatta catcatcgtc
     5761 tttgatttca ttttcctttt tatgtgatat taaccttagg tattcaccca tagtgcccat
     5821 aatcatgcag gctgtaattt ccgattttat actcttttta acaacgccct cctccttgcc
     5881 ctggtctatt atatcacata tcagaccata catttcagaa atttttaata ttattttctt
     5941 gattgtttct tctgatataa tgtttttgct tgatagaata ttgtctatgg acatatcctc
     6001 aaaatctgat tccattttaa gttttccatt gaataaaatc aagagtttgt tattgaatgt
     6061 atcatccata tttgaaacgt atcttatgtt gtcaataatc acattcacat attcaatgca
     6121 tgcttcatca aatatatctt gtttgttttc aaaatactca tacagggttc ccttacccac
     6181 acctgcatct attgcgatgt cttccatttt tgtattttga aaccctttat ccatgaataa
     6241 agccatggct gcttgtaata tatcctggcg tttttgattt ttttccacaa tgcacctcct
     6301 atgctctcac tgtatttgtt cagtaatttt gtctttcttt atccttctct tgaaggattc
     6361 agatatatca tcaaatatag tatacataac aggaactatt attaaagtta aaactgttga
     6421 cagggataat cctccgatta ccacaacacc catagactga ataagttcag ccccttcgcc
     6481 tatgcctaat gacattggca ccagccccaa gatagttgtc aatgcagtca tcatgattgg
     6541 acgtattctt atgggtcctg ctttcatgat tgcagcatct ctcgtttctc cccttcttct
     6601 cctgatgttt atataatcaa ccagcactat tgcattgtta acaacaacac ccaccaaaat
     6661 tatcagtcct attatgccta ttacatttag agggagccct gcaataaaca gtcctataaa
     6721 tccgccggac aaggcaagag gcaccgaaaa cattattgac aaaggctgaa tcagcgattc
     6781 aaactgggct gcaataatca tataaaccag ggcgattgcc atgagcatta ccaggaacaa
     6841 gtctgtaaaa gtttcctgta tctgctcaat ctctcctcca tacatatagg aataaccgta
     6901 gggcatttca tatactttaa ggagatcttc tatttcaacg gaagcagatt gaacatctct
     6961 tcctacaaca cttcccgata ctgttaaaac tcttacctga ttctccctgt atatgcttac
     7021 gggtcctttt tcaactttta tttcagctac ttcagacagg ggcacgctgc ttcccgcagg
     7081 ggtttcaacc ggcagcattt ccaaatatga gaggctttct ccataaacat tgtctccttt
     7141 aagcacaaca tctatttcgt caccgtcaac cttaaatttt gtaacatttt tgcctgagag
     7201 agtatttata accgaatttc ctatttgagc cgttgttaaa ccgtactgag atgcaattcc
     7261 cctgtctgct attatctgta cttgaggaat gccgtcttcg tagcttgttg aaacttctct
     7321 ggttccttca actttttcaa ttatattttt aaagtcatct ccgatggctt ttaaagtatc
     7381 catatcgtcg cccttaatgc tgatacttat ggctccggtc agggtcccca tcatcatgga
     7441 tgtagattcg gcaacactta tttcagctcc cgggatgtcc tttgtcagag ttcttatttc
     7501 atcactgatt tgttttgcgc ttttgtccct ttcactcaag tcctttaata aaacattcat
     7561 cgagccggtg gaaccgcctc ctataccgta cattcccccg cctccggcag cggtaaatgt
     7621 aaactcaatt tccggaattt cttttattga atcttgtatt tccttcaaca aagcatctaa
     7681 ttcctctgtt tcagagccgg caggcatgct tacgctcaca ttaatcatac cttcatcagt
     7741 tgcagggaag aattcagctc ctacagatat aaccatcata acgctgataa taaacaatgc
     7801 tatgctgctc aatatcgtta taaaacggtg ccttaaactt aagctgagga cctttttata
     7861 ggaaacaacc aggggcccct gctttttttc ctgttcctct tccgattcaa ggtttttaac
     7921 agaaataagc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnataagaag
     8041 agcttaaatt gtatccctta atcgcttctt cccttaacat tacttcaact tcctggtcta
     8101 ggcctcccgt tacagaggca gaagcagtgc cctctattcg ttcaaagcgg ggtgatatta
     8161 catcctctgc aatcttttgg gttgtgtgga gatctccttt gcttgaaaca gccaattgta
     8221 ctattggaag agcattgggg tccagcttta acgccagggg agaagatgtg ccctcaggca
     8281 ggaaaccttt tataacatca actctttccc ttatttgaag cattacctca tccatgtcag
     8341 tgttataagc aaactgcatc aataaaagcg agcttccttc gcttgaaatt gaaataactg
     8401 cttcgatatt ttcaaccgtg gacaaagtct gttccatagg ttttgatatt atactctcta
     8461 tttcttcagg acctgctccc tgatagtttg tttgaaccat tatatagggc agatccattt
     8521 gaggaagcaa atctatctga agcttgctga aggataccac tcctatgaca atcactatta
     8581 aaatcagcat ggtgattgta acgggctttt taaccgagaa ttttgccagc attcctattc
     8641 accccttaca acctttattt tgctgccctg gcttatgtaa tcctgaccct tcacaataac
     8701 tttatctccc tgggagatac ctgtttttat ttctatgtaa gtacccgtat caagtcctgt
     8761 tgttacctct ttctcaacag cagcctcacc ttctgccaca aatacaaact gtttgccttc
     8821 acgttcctta acagcttcgg ttcttacggc aagagcattt tcaatattat ctgtcttcaa
     8881 ataaacttta gcaaacatgc ccggtttaat tgttccgtca ttttcaagca ctatacttac
     8941 cgtataaaga ttagtcatga tatctgccga tgagctgaca ctgttaataa aagcttcatt
     9001 tttataaccg gcagaagaga tttcaaccaa tactccatca ccggttttaa ccttgttaac
     9061 aagactggcg gctatgccaa attcaatagt cacactgtct gaatcgacta ttgtaacagg
     9121 cggattcgca gatgttgcat attctccttc aactatgttc acgcttgata ccactcccgg
     9181 tataggggct ttaacttcag cattcttcag cattgatgct gaattggagt aagccacctt
     9241 tgcctgttca aggcccattc ttgctgcctc cagggcttca tccgacgctg ctaatttcgc
     9301 ctgctcatat tgggtttcag gaactgctcc ttctttgtag agcttttcga ttcgttcaag
     9361 attatccctg gcactttgta tttgagcaga acttacttca aagccggctt ttgcagcttc
     9421 ataagctgta tatgcctggt tcacctgctt ttgtatatca tctttgtcca ttacaaacaa
     9481 gacgtcatcc ttttcaaccc tgtcacccac atttacgttt attttttcta cctttcctgc
     9541 catcacaggt attacaaaca catccttgtc tgcaaatacc ttagcagtca tgatgttttc
     9601 tatgtaaagc tccataggct taagtgtttc cacttccaca ggcgtataag cttcttcttt
     9661 gtctgcattt gtttcttggc ccgctgtgca gcctgcaagt acaaatacga taattaataa
     9721 taatgtcaat agtcttttca agttagatag cctccatttt tcgaccgacc ggtcagtttt
     9781 aacaaattat aacacctgta ttttatgctg tcaatcaaga cactataaat aatatatcga
     9841 agggatgggg gtattgtcat atgagtttac atatttaaat ttatgtaaat cgattttgtg
     9901 gtatagttct tttgttttat gttcttgtag tattggttaa catatagaaa tttatataaa
     9961 caagtataat tattgttgac tttgtatttt tatggtagta tactttattt taaataagag
    10021 gtgcgacatg gaaaacaaag ttttgttcgt acaattacat caccataggt gcagagtttg
    10081 taagtgataa tcacattaca agctctattg gtcgttttac aaaagggctt gtatctgtgg
    10141 tgaatggttg ataagtggaa ttttaagcca tccgggtata agccgggtgg ctttttgaat
    10201 gtccccaaat agataaactg attaacgaag accgtgttgc taaatctgcg cgacgcatat
    10261 caaggaggat taagagagtg aattttttaa aaatcgaaga tgaaatgaaa tattttcaaa
    10321 ggatttttat gctaagaaaa gaaatcgaga attttctgtt taaagaaggc tatatgaata
    10381 ttgagccgtc catattcgag gaatatgatg aatttacatc cgttaattca agaatagaca
    10441 agaaatcaac agtgaaaatt ctggaaagca acagtgatat tttaatttta tcaccggata
    10501 ttacaaccgg aatcataaat aagttcatgc caaggtggga ctacgactta atgctgaaaa
    10561 tcttttatta cggcaaaaca tacaagcata acggcaatgg cattaaggaa aaaagagaaa
    10621 tcggaattga aatcataggg gataacagcc agaatattga ccaaaacaca ttaatgactg
    10681 ctgaaagaat aataagcaga tacagcgaca attacttgct tgaaatcgga aacagcaagt
    10741 ttcttaaggg tataatgctg gcctgctcat ttaacaagga ggattatgac aatatactgg
    10801 atttattgta tttgaaaaac aaacaggaat tggatgacta tttgtctaaa ctgaattaca
    10861 aagagccaat atgcacattg cttaacatcc ttgacctgga aggtacatat gaagaaatta
    10921 ccggcaagct agaagggctg tatttgaacg atatgatgaa ggccggtctt caagagctta
    10981 aaactgttga tgattatatg aaaaacataa cagataaaat tatttacgac ctttcaatgg
    11041 tatcagaatt gagctattat gacggaatta tattcagagg atatattcat ggttccaata
    11101 cagaaatcat aaagggcggt agatatgact catttactga gcagttcgga gtaaaaatac
    11161 cggcaatagg attcaccgtg gagctggatg aactgctgaa agccatatac aaggagggat
    11221 gataatgagt ttgacaattg caataccaaa gggaagatta ggcaacagga ctattgaaat
    11281 actaaaagct gcaggcctgg gcagcgcaat cgatgaagat tcgagaaaat tggtttttaa
    11341 cagcgagaat cgaagtatta aatttatact tttaaagaat tccgatgtaa taacatatgt
    11401 ggaaaatggg attgcagata ttgggatatc aggaaaggac atgatcatgg aaagctccgg
    11461 agacttttac gagctttata aaatgaatgt gggcaggtgc aagatgtcgg tggccggatt
    11521 taaggacaaa aaagttttca atgacgacac aatcttaaag gtggctacca agtatccgga
    11581 aacagcaaga aaatttttca acagcagagg gcagaaaatt aaaataataa aattgaacgg
    11641 ctccatagag cttgcgccga ttttaggatt atcggatgta atcgttgatt tagttgagac
    11701 gggcaacaca ttgaaagcca atggccttga ggtttttgaa gatatgtatg atatatccgc
    11761 agttataata tccaataaaa taagttataa attcaaaaga aaagagataa aggaaataat
    11821 tgaactgata aggagagaag ataatggtca ggttaattga aaacaatata gaagagtatt
    11881 tagctgaaat tcaaaatcgg aatcaagatg actacaggga agtttacaaa gcagttgatg
    11941 aaatacttga agatgtaagg aaaaacaagg atgaagcatt gaaaaaatac accatggaat
    12001 ttgataaggt aagcttgaac aatttggaag ttagtgaaga cgaaataaag gaagcatatc
    12061 aaacaattga caaggagctt ttggaaacca tcaagaaagc taaggaaaat atttatgatt
    12121 accacaaaag acaagtaaga aggagctaca aatacaaacc tgagggaaag gacataattt
    12181 taggccagct tataaatcca attgaaaagg ttggaattta tgtgccggga ggtaaagctg
    12241 cctatccatc cacggtattg atgaatgcaa tccctgcaaa aatcgccgga gtgaatgaaa
    12301 taattatgat aacccctccc caaaaagacg gaaagctgaa ggattcaata ttggttgctg
    12361 cagacatggc aggtgttgac agaatattca aagttggagg agcacaaggt ataggggctt
    12421 tggcttatgg aacagaaaca attcctaagg tgtataaaat aaccggtccc ggaaacattt
    12481 atgttgctgc agccaagaga cggatatccg gttttgtggg aatagatatg attgcaggac
    12541 caagcgaaat attaattatt gcagacaaaa ccgcatctcc cgcctacatt gcagcagata
    12601 tgatatccca ggcagagcat gatgaaatgg cagcctgcat tttaataaca aattcctact
    12661 tgcttgctga taaggttaaa aaggaattga aaaaccagtc tcagttattg gaaagaagag
    12721 aaataataga gaagtctttg aacaactacg gtgcaataat aattaccgac agcatgaaac
    12781 acagcattga tttggccaac gaaataggac ccgagcattt ggagcttctt acaacaaacc
    12841 cttttgaaaa ttataaaaaa attaaaaacg caggagccat atttttagga ggaaacagtc
    12901 ctgaacctgt gggagattat tttgcaggtc ccaatcacac cctccctaca aactccacat
    12961 ccaagttttc atcagggctt agtgtagacg actttataaa gaaaacttct ctgatttact
    13021 acagcaggca ggctttgctt gaaaacggag gccgcatagt gaggtttgca gaaaatgaag
    13081 gtctgacggg ccatgcaaat gctataaaaa ttagaatgca ggggggagaa aatgattaaa
    13141 ctgaaacaaa gtgcagaaag aattgaggct tattttgtaa acaatgtaga ttgcagggta
    13201 aagcttgatg ccaatgaagg aagcaactat ttgcttaagg atggatttaa aatagacagt
    13261 ttcaaagtaa acttataccc tgacagcgac tgcagttcat taagagaaaa aatggctttt
    13321 tattatggct gcaaggctga aaatataatg gttggaaacg ggtccagcga aattatcaat
    13381 acggtaataa acgcttactg cgaaaaagct gacaaggtta tgagttttgt cccttccttc
    13441 agtatgtaca aaacctactg cgatttgtgc tctgctgaat atgtgggtgt agactcagaa
    13501 gatgattttt cacaagatat aaataagctg ataaaagaag caaaaatgct cgacccgaaa
    13561 ataatcatca tctgcaatcc caacaaccct acaggttatg taactccaat ggaggatgtt
    13621 attaaggttt tggaaaattt taaaaattcg ctggtcattt tagatgaagc ttatgctgat
    13681 ttttcaggca actcggcact tggattgatt aatgattatg aaaatttaat tgtaatgagg
    13741 acaatgtcca aggccttcgg actggcaggg cttagggtcg ggtgtctgat agcggcagaa
    13801 aaaacagtta aatacatttg gaaggttaag cttccttata atgtaaatat attgtctcaa
    13861 tatgcggcag aaaaggcatt aagtaattcc ggccgagttg tagaatatat agaaagtgtg
    13921 aaaatcagaa gagaagagct tagcagtgaa ttgaaaaagt tagatttcct ggtatatcct
    13981 tctggagcaa actttttgtt tgtaaagccg cccatacaaa atttgtttga aaaacttatg
    14041 gaaagaggaa tattaataag aaaattcaat gatacctatt accgcatatc tgtaggggaa
    14101 aaggaagaaa acgaaatatt aactgaagag ttaataaaaa tcaggaggga gcaatgagga
    14161 aagctgtaat aacacgaaat acatcagaaa caaaaattca aatcgagtta aatttagatg
    14221 aaagcggagc aagagaaatt gatacaggtg tgggattttt ggaccatatg ctgacattgt
    14281 tttgttttca tggcaaattc gacttgaata ttaagtgcaa aggggattta aacacagata
    14341 cccaccacac atcagaagat gtaggaatag cattaggtca agctttcaca aaagctttgg
    14401 gcgataaaag aggcattgaa agattcgggg ctgcctatgt gcccatggat gaatctttgg
    14461 caagagttgt tcttgacttg agcggcagac cttgcctggt ttacaattta gatttcaaga
    14521 aggctcaaat aggaaccatg gcaacagagg atttcaaaga attcttccaa ggatttgcaa
    14581 ataactgctt gtgcaacctg catatagaac tgctgtatgg cgagaatgac caccataaaa
    14641 ttgaagctgt attcaaagga tttggaaggg caatgaaaaa ggcagtggag gtcacatctg
    14701 acagccttca atccacaaag ggggccctgt aatgatactg ttaccggcaa tagatataaa
    14761 agacaacaaa tgcgtgagac ttactcaggg aaagtttgac aagctagtag tgtattcaga
    14821 cgaccctggg gaaatggcaa gaaaatggga gtcgatgggg gctgaatacc tccatgtagt
    14881 tgatttggac ggtgctaaaa atgaaggttt tcaaaacaga aggtcaattg aaaaaatagc
    14941 caaatcaata tcaattccca tacagacggg gggaggaatc cggagtgagg aaagagtaaa
    15001 agatttgatt aatataggtg ttgacagggt tattgtaggc actatggcaa ttgaaaacag
    15061 agagcttctt acaaagcttg tgaaaaatta caaagaccac atagcggttt ccatagatgc
    15121 attaaagagc aaggttgcaa caagaggatg gcagagggtt gagaatgtgg attcaataga
    15181 aatgtgtaaa gcattggaat ccatggggat aagaaccatt gtctacacgg atatttcaaa
    15241 agatggaatg ctttcggggc cgaatttcga catatataga gaattatcaa caaaaacaat
    15301 cataaatata attgcatccg ggggtgtcac tacaccggag gatataaaaa agcttagaga
    15361 aatgaactta tacggggcaa ttatcgggaa agccttatac gatggcagat tagatttcaa
    15421 ggatgcccta aaaatataat tatcaggagg aaaaaaatgg cagtaagaat aataccctgc
    15481 ttggacgtaa taaacggaag agttgttaag gggacaaagt ttaaagatat taaagatgtt
    15541 gatgacccgg ttaatttagg caaatactac agtgattcag gagcagatga gttagtgttt
    15601 tatgatatta ctgcatccct tgaaggcagg aaaacttcgc ttgaatttgt aaaaaaagtt
    15661 gctgctgaaa taaatatccc cttctgtgtg ggaggaggaa tctcatccat agaagatttc
    15721 aagcatgttt tagaaagcgg tgcggataag atatccatta attcagctgc agtaaaaaac
    15781 ccctatttaa taaaagaagc atcaggaatc tatggcagta agtgccttgt gctggctcta
    15841 gacgtaaaaa gaaatgaaga aggctcatgg agtgtctacg tcaagggggg aaaagaaaaa
    15901 acatccctgg atgctgttga atgggttaag acaggcgtga aattgggagc gggagaaatc
    15961 gttgtaaaca gcattgatga ggatggtatg aaaaccggat acgacttgga actcctgaaa
    16021 agagtaaaag ctgctgtaaa tgttccggtt attgcatccg gaggtgcagg aaagtacgaa
    16081 cattttttag aagctgttaa atatgcagct gtagacggcg ttcttgcagc ttcagtattt
    16141 catttcggag aaataaaaat acccgaactc aaaaaatact tgagggaaaa cggattgtag
    16201 gcaggaggat acaatggaaa ctcaaatgtt aaatgggata aaatttgatg aaaaaggttt
    16261 gattcttgca atagcacagg attatataac aagtgaagta cttatggctg catatatgaa
    16321 cgagaaggct ttagaaataa ccttgaagga aaagaaagtt tgttatttct caagaagccg
    16381 taattgttta tggagaaagg gagaaacatc aggacatgtc cagcatttaa agggtctcta
    16441 ctatgactgt gatgccgatg caattttatt gaaggtagag caggttggaa atgcatgtca
    16501 cacaggggca tactcatgtt ttttcaataa ggtttacgaa gaggacatag ctgacaaatc
    16561 aattattaat aaggtgtact gtcaaataca aaaccgaaaa gataatccca aggaaggctc
    16621 atacacaaac tatctgttta atgaaggact tgataaaata ttgaaaaaaa tcggtgagga
    16681 aagcagcgaa gtattaatag cagcaaaaaa taaaaacaaa gaagaactta tttatgaaat
    16741 ttccgattta atttaccact ccttggttct catggtaagt gaaggtgtaa ccattgatga
    16801 tctgcagaaa gaactgacaa aaaggcatag atagctggaa aggcagcttg tatagaactg
    16861 tctgacaaaa caactattta taacaaagtg tgcaaaattt caagtaatgt gtggtttgtg
    16921 ctgtattaat tccggatatt ccacatatta cttgaaattt cgaccatatt ctacttaaaa
    16981 atcatcttcc cctaaatata tttcacacaa taattgaaat tttgagcaag ttaaagcctg
    17041 tttattccaa ttttaaagct gaatggacat gctattttcc ctgcactttg gtttgaaaaa
    17101 atagggttag ggcaccttta atatgctctt gctgcgtaaa tcatgtgttt tgcaggtttc
    17161 ccgcagatat gacacttgtc tcccaagtct tcctgctcaa aaggaataca cctgattgtt
    17221 gcgccggttt cttcttttag tttatcttca cattcccttt caccgcacca catcattttg
    17281 gcaaatccgg gtcggttagg aagatctttt ttgtattgtt catagtcttt tacaatataa
    17341 gtatgggctt ccctgtgagc ttttgctttt tcaaataaat cattttgcat tgtttcaagc
    17401 atgttatata ttgtgtcttc aatattatca agtgaaagag tttctttttc taatttatct
    17461 cttcttacag caacagcctg attattgtca atgtctcttg gcccaatttc aatacgaata
    17521 gggtaaccct tcatttcata ttgattgaac ttccagcccg gtgagtattc tgtgctgtca
    17581 tcaagttcaa ctctgattcc tttagctttt aatttttcat ataattcatt tgctttttcc
    17641 aatactcctt ctttcttttg tgctatcgga attattacaa cttgaacagg tgccatttta
    17701 ggaggcagta ccaatcccct gttgtctgaa tgaaccatta tcagaccgcc gatgagtctg
    17761 gtggaaatac cccaggatgt atgataaggg tattgttcct gtccgtttct gccctggaat
    17821 tttatttcaa atgcttttgt aaaatgctga cctaaattat gtgaagttcc tgcctgtaat
    17881 gcctggccgt catgcatcaa tgcttccatt gtatatgtgg catgggcgcc tgcaaatctt
    17941 tccttgtcgc ttttttgtcc cacaacaaca ggtatggcca ttaagtcttc agctacctgc
    18001 ctgtatatat cgagcatctg caatgtttct gcctgagctt cttcctctgt ttcatgaaga
    18061 gtgtggcctt cctgccataa gaattctgac gttcttaaaa aaggccttgt tgttttttcc
    18121 catcttacaa cgctgcacca ttgattatac aagtatggca ggtctctgta tgagtttagc
    18181 cattttgcat acatatggca aataatagtc tctgacgtag gtcttataac cagcctttct
    18241 gccagttcct ggcttccccc gtgggtaacc caggcaactt caggagcaaa tccttcaaca
    18301 tgctcctttt ctttcgtcaa taagctttcc ggaattaata acggaaaata catgttctta
    18361 tggccggttg ctttgaactt ttcatctgca tattcttgaa ttttctccca caaggcataa
    18421 ccgtaaggtc ttattaccat aaaacctttt acaggtgaat agtcaaccaa ttcatttttc
    18481 attattacat ctgtatacca cctgctgaag tcttcttcca taggtgtaat ttcttttacg
    18541 aactcttttt ctttcttagc cattctttct cctttcaaaa attaaaccgc ctcctgaacc
    18601 aagaggcgga tatccgcggt accactctaa ttgctgctat taacatcaca gcatctcatt
    18661 tatataaggg ttttgccctt tgggttgttg ctcccaaata ctcttgggcg ggttcataat
    18721 acagggggcc gaaggccttc caccaacaac cttctctctg atagaccatg tataattact
    18781 gttccccatc aacgtaatta ttaatttata tacataatat aaaaatttaa tcttattgtc
    18841 aacaattttc aggagttttt ctgcaatt
//
LOCUS       DAOPUT010000022        17921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3197_length_17921_cov_17.685492, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000022 DAOPUT010000000
VERSION     DAOPUT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 17921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17921
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3197_length_17921_cov_17.685492"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..83)
                     /locus_tag="PKK75_03550"
     tRNA            complement(1..83)
                     /locus_tag="PKK75_03550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Leu,seq:tag)
     gene            complement(115..1008)
                     /locus_tag="PKK75_03555"
     CDS             complement(115..1008)
                     /locus_tag="PKK75_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNZ82702.1"
                     /translation="MISNEDLIRLHLINGLGRKTISKILSYIEFKNIRLNNFSEVLKM
                     ISQMNLKRLKIDEEKIEREAAVIFECCKKNKIKIIGVYDKTYPYKFRSIEDKPLILYA
                     DGDLELLNDRNNIAIVGTRTPSQEGYEISSILAERLTEDNCCIVSGFAAGCDEAAHYG
                     CLQAKGRTVAVVPSGHLNVLKQNRSLYNTIIYNGGLIISELPPPYKAEKNSFIDRNRL
                     IAAVSEGVIIVEGGLRGGTSHMVNYAKEYQKPVAYSVNLCKITGQTLTFNDIDIIDSF
                     EELVKFKNKSFKNVLDKAISQ"
     gene            1240..1755
                     /locus_tag="PKK75_03560"
     CDS             1240..1755
                     /locus_tag="PKK75_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82703.1"
                     /translation="MNRRRNMPYIARNKIKRAKKPEILQLLSEAELDGDNSNIQTGFV
                     SVSVNTALGAVPVRDAVVTLYVTDLEGNEEALYHLVTDISGKVPKMALPVVYNPQDPL
                     GSRAFFFSTYNLRIQAIGYYTSNIIDLRVFPDTTTNFTVTLIPVSEGGDEDEGERTIV
                     IPSSPIDESNV"
     gene            1765..2658
                     /locus_tag="PKK75_03565"
     CDS             1765..2658
                     /locus_tag="PKK75_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNZ82704.1"
                     /translation="MSVTTIQQTFPESPLKLGDDSQYVLIAKEALNVISVNYPAIPVL
                     VAVNTVFDENMEAAVKEFQNIFDLPVTGIIDMATWFQIGTVQATVLRLAELAGRGVLV
                     GDTVEDITEVEEGVQVLPRVQMVQFFLNVLSAFYDSINAVDINGILGPETRNGIREFQ
                     KTMGLPTTGIIDEETWIAMYNSILGILRELPPVAVQLPSLLYTGTVYSEGSERPGVLI
                     IQEILAYISTVNSDIPFVQPDGIFGPETRASVSAFQRYYGLEEDGIVDEETWNKLISV
                     YRELRFGDTNLLPDAGESLIQ"
     gene            2677..4092
                     /locus_tag="PKK75_03570"
     CDS             2677..4092
                     /locus_tag="PKK75_03570"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNZ82705.1"
                     /translation="MSIDNVNVFIPEAGLPHVIVPFPLYITVHLGPPDEDALNVTVPY
                     LDYIKNVGSSELYPTWPEEALRANINAISSIAMNRIFTEWYRSQGYEFDITNTTQYDQ
                     AYVHERGIFDSISNIANEEFNRYIVRNGFIEPMFATFCDGRVSVCDGMHQWGSVSLAN
                     QGYTSEDILRYYYGDDITLVDSTTAVVITGTYPGEPLSLGDSGIDVFRMQHSLRNISD
                     SFPLIPRVEITGFFDEETENAVKVFQEVFGLPVTGVVDSQTWYRIRFTYIAVERLAEL
                     VSEGLSYEELRQLYSNIVLEGATLPIVAYIEFFLNVISTKYDTIEPVEIDTFYGPDTA
                     RAVREYQIIMGLNPTGIADQETLNLLYRDAYSVLTSTPVEELTLPFLPYTGVDLTQGM
                     GPEYPRIVLLEIMLNTISLTQPEIRPVEVGGGFDSNTRAAVIAFQSLYGLPPTGVVDE
                     ETWNRINEVYQSVIASNTTGS"
     gene            4108..4626
                     /locus_tag="PKK75_03575"
     CDS             4108..4626
                     /locus_tag="PKK75_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNZ82706.1"
                     /translation="MVNLENNTNFILEIQNYLNNVNHAWHMIELTGVYDQATRNAVLI
                     FQNANGLPPTGYVDVPTWAVLASKNNEYLRKTQMPGKITVSNPEFKDVKTGDQNDLVY
                     GIKVMFNNFSRRYANYPELEVTNLYDEKTEEAVILFQQRSMLPVTGIVDLKTWNSFCK
                     IYEVCRLYEERH"
     gene            4886..6757
                     /gene="htpG"
                     /locus_tag="PKK75_03580"
     CDS             4886..6757
                     /gene="htpG"
                     /locus_tag="PKK75_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNZ82707.1"
                     /translation="MAKKQFKSESKRLLNLMINSIYTHKEIFLRELISNASDAIDKSY
                     FLSLTDKNIPFNKDDFYIRITTNEEERTLTVSDTGIGMSKDELDSNLGIIAKSGSFDF
                     KEDIEIKESVDIIGQFGVGFYSAFMVSDKVTVISKKADSDEAFKWESTGADGYTITPC
                     SKETAGTDIIMKIKPSTEDEDYDEFLDSYNIKSLVKKYSDFIKYPVKMMIKKRKLKDG
                     SKNEYEEYFEDEILNSMVPIWRKNKSELKEEDYENFYMEKHFGYEKPLKHTHVSVEGV
                     ISYNAILYIPARAPFDFYTKEFEKGLELYSNGVMIMKKCGELLPDYFGFVQGLVDSPD
                     FSLNISREVLQHDKQLQFIAKKLKEKIKSELFAMLKDERESYEKFFQSFGRTLKYGLY
                     SEWGSNKETLQDLVMFYSSHEKKLATLDEYVSRMKEDQKYIYYAAGESVDKLARLPQA
                     ELVADKGYEILYFIDEIDEFAIKVLFSYKEKEFKNISSSDLDIKAEDDNTADAEENKE
                     IFAFMKEALGDRVKEVRSSKRLKTHPVCLSAEGDVSIEMEKVLNAMPDNPADIRAEKI
                     LEINTGHKVFEKLKDAYDNDREKLKKLSNVLYNQALLIEGLTIEDPVQYANDVFELM"
     gene            complement(6820..7704)
                     /locus_tag="PKK75_03585"
     CDS             complement(6820..7704)
                     /locus_tag="PKK75_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNZ82708.1"
                     /translation="MRRNKKLRIRRTIIVVTALLLILFGAFLIIEKLGNRGEEPSNLP
                     DNPEEEETSVEPENPQEENPQEENPQEEEPEDSQEPPEPEKPEPKEEDPVQEQPDDEP
                     GITIENPEKLDVLVNRKRNLPENYVPEDLVTLGEVPTVLSNPEVNQLRSAAYEALKEM
                     FTAASETGNELHARSGYRSYYTQSSLYSSYVNSYGKAAADKYSAKPGQSEHQTGLSID
                     ITCEAVNYKLDTAFAETEEGKWVAENAHNYGFIIRYPKGKEKITGYEYEPWHIRYLGE
                     DLAEKLYESGLTMEEYFQ"
     assembly_gap    7729..7828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7829..8266)
                     /gene="rimI"
                     /locus_tag="PKK75_03590"
     CDS             complement(<7829..8266)
                     /gene="rimI"
                     /locus_tag="PKK75_03590"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNZ82709.1"
                     /translation="MGDVIVRGMLQKDIDQVMNIERICFSMPWSKASLENELTNELAY
                     YQVAEVSGEIAAYMGMWKIIDECHITNVAVLPEYRNKGIAGMLINKMIEICKSSEISY
                     MTLEVRISNEPAINLYKKFGFYAVGKRPNYYVKPLEDALIMWME"
     gene            complement(8276..8965)
                     /gene="tsaB"
                     /locus_tag="PKK75_03595"
     CDS             complement(8276..8965)
                     /gene="tsaB"
                     /locus_tag="PKK75_03595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNZ82710.1"
                     /translation="MKILSIESASVAASCAVSEDENLLGEYTLSHKKTHSEKLMPLIE
                     ELMEDLGLKLKDIDFLAVSEGPGSYTGLRIGAALAKSLAFAGNLRIIGVPTMKSLAAN
                     IYDEDKLIVPVMDAKAGRIYTGIYRWDNRELRETEEQFACNIDELIQILNKYEEPVVF
                     NGDGSVNYMERILNNLNRKSYFAPEACNYLKASTLGFIAYKMAVAGDTVTADEFKPRY
                     LRLSQAERNRK"
     gene            complement(8946..9443)
                     /gene="tsaE"
                     /locus_tag="PKK75_03600"
     CDS             complement(8946..9443)
                     /gene="tsaE"
                     /locus_tag="PKK75_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNZ82711.1"
                     /translation="MKEKNEVRNNMKITANNLKDTEKLGKIIAKCLEKGAVICLDGEL
                     GAGKTSLTQFIAKEFDVKEYMVSPTFTIIKEYDGKLPFFHIDAYRMGSEDDMYDLGFD
                     EYIYSEGVTVIEWADLIRGMLPEERVNIKIIRIDDTKREINIEGKGPVYEKLVRELKN
                     ENTFN"
     gene            9771..11117
                     /gene="murC"
                     /locus_tag="PKK75_03605"
     CDS             9771..11117
                     /gene="murC"
                     /locus_tag="PKK75_03605"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNZ82712.1"
                     /translation="MKYNFNHIYFIGIGGISMSALAEIMLEEGIKVSGSDRSDSKIIK
                     NLQKSGADIYIKHDSKNIGDDIDLVVYTSAIAEDNPELLRAKELNIKIMDRAEFLGAV
                     MKNYSHAICVSGTHGKTTTTGMLSSVLIDTDLAPTIFIGGEMDSLGGNLLHGSYDLLL
                     TEACEYKRNFLKFNPTMEVILNVEEDHLDYYKDLKDIESAFVEYAEKLPPDGHLIVNE
                     AYSHLFNNIKCKVVTFGLDKGDYYAGNTRLIPEPSYTLMNGDKEVIEINLKVFGEHNI
                     LNSLAACAACLSLGIEPETIAKGLNEFKGTHRRYEYKGEINGAVIIDDYAHHPSEMKA
                     TLKTARSYAGGRIITVFQPHTYTRTIKLLDGFAEALSMSDEAILVDIYAAREIDKGEI
                     HSMDIINKMKNYSKNGIYAENFLEAADIVRSMAKKGDIIITMGAGNVVDIGEMLLE"
     gene            complement(11224..12108)
                     /locus_tag="PKK75_03610"
     CDS             complement(11224..12108)
                     /locus_tag="PKK75_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNZ82713.1"
                     /translation="MEFITWFKEKVKIVDEYLSRIIEERENPQKIIYEAMNYSLLSEG
                     KKLRPVLLLGAYELFRDDLKEALPFACAMEMIHTYSLIHDDLPAMDNDDYRRGKLSNH
                     KKYGQATAILAGDGLLNKAFETGLEASINSKSMNAVKALKIIADSSGTEGMIGGQVVD
                     MEGDEIINSIEDLKYMYSLKTGAIIKSSVIAGAQLAGADEEEMRQLGSYAEKIGIGFQ
                     IEDDILDVTGTPEKLGKAVGSDAANKKITYLTFKSIEEARQDVEKFTQEAIESLSMFG
                     GRAEYLVELAKFLTNRER"
     assembly_gap    12132..12231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12232..13924
                     /locus_tag="PKK75_03615"
     CDS             <12232..13924
                     /locus_tag="PKK75_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ82714.1"
                     /translation="KILSLLLAVMMIAVVFAGCTQAPPAEEPPAEEPPAEEPPAEEPK
                     GDQILKFGATGFDGVFNPILSDNVYDAYIESIIFEKLITNNIEGEYIPNIAEWVLSED
                     KLTYTFTLKDGIKFSDGSDLTAEDVAFTYMTIAHPDYNGPRAYAVNNFVGYDEYHAGE
                     IDVFEGIKVIDEKTISFTFKEGMAAPANIECFVYGIMPSDYYAFDTWEDFLAFNDKPM
                     GSGIMVFDSWAPNEFILLKNNTNYWDPDNAAKIEGVLVSEVPDESILSALQTDQIDFA
                     QIAATGENLAAAEAMDNIQVANYLANGYTFMCFNTTRPQLSDARVRQALMYALDRESF
                     IDVQYGEGLGSVGMAPISPSSWAFPDPSELNPYAFDMEKAGQLMDEAGWIMGSDGYRY
                     KDGQKFAVNWLVYTDSAWPGTLSGMAADTWKQLGVELTIELMDFNTVAARTMDAEPGE
                     KDFDIYTMGFSLSIDPDPTGALFDDDAYVAGGFNASGYKNPEAMELVRQGKAEFDTAK
                     RAEIYKEWAKIMNAEIPHVIIAYRSEIWGINNRVDGMVIDTYATWDQSLKNVTLK"
     gene            13996..14964
                     /locus_tag="PKK75_03620"
     CDS             13996..14964
                     /locus_tag="PKK75_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82715.1"
                     /translation="MRNYIIRRIIQMIPVFIGILFILFFILEQAPGTPVSNMMHPRMT
                     PEQKAELNEKLGMDIPWYERFVNYVKEALLEGNLGYSNTHKRPVAEVIGDYVGPTLLL
                     AVVSLCISIIIGIPAGILSATKQYTAVDNILTVVSFIGISIPSFFFGLILLKVFAVDN
                     QIFPLFGIRDPLLMSDNIFDNIKDVAWHLVLPSIVLGLSSTASFMRYTRSSMLEVIKQ
                     DYIRTARAKGLKEKTVIYRHAFRNGIIPIITLLGFWIPSLLSGAVVTETIFGLPGLGK
                     ISVEAAMKRNYPLILGINSMLAILTLIGALVADILYAVADPRIRYD"
     gene            14977..>15581
                     /locus_tag="PKK75_03625"
     CDS             14977..>15581
                     /locus_tag="PKK75_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82716.1"
                     /translation="MAKDKTNKNYIRNQSYWGEVFFRLRKNKMAMLSLYILVAIILLC
                     IIIPIISPYEIQTTNLAGKDQSPNSKHLLGTDLIGRDLFTRLFYAGRISLGVALAVVA
                     IECVIGVVLGSLSGFYGGIIDGVIMRIAEIFMSFPFMIITITIVAVFGNSIFTLILVL
                     ALLSWPSIARIVRGQILSLREMEYMEACEALGISDIRRIFR"
     assembly_gap    15582..15681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15686..16672
                     /locus_tag="PKK75_03630"
     CDS             15686..16672
                     /locus_tag="PKK75_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82717.1"
                     /translation="MSKELLRVNNLKTYFYSDRGIVKAVDDVSFYVNEGETLGIVGES
                     GCGKSITCMSLVRLVETPPGKYEGGEIIFNGEDMLKVSDTRIRQIRGNDISFIFQEPM
                     TSLNPVFKIGRQISEALILHRGMSKDEARKESIRMLELVKIPNPERVVDDYPFALSGG
                     MRQRAMIAMALACEPMLLIADEPTTALDVTIQAQVLDLMNELKKKINASIIFITHDLG
                     VIAEMSDRVMVMYAGKIAEMASTVEIFKNPKHPYTMGLIGSKPDMATESARLNVIPGN
                     VPDLSNLPAGCPFHPRCDKAMDICKKDFPAEKTLYDGHKISCWLYTGKEELQ"
     gene            16669..17655
                     /locus_tag="PKK75_03635"
     CDS             16669..17655
                     /locus_tag="PKK75_03635"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNZ82718.1"
                     /translation="MSEYLLTVNNLKKYFPIKTGLVKRTTGNVKAVDGVSFNIKKGKV
                     LGIVGESGCGKSTLGRTILRLLTPTEGEVFYEGKSVFDMDHKEMNKMRTKMQIIFQDP
                     YSSLNPRLPVYDIVGEAMLQHSIVKNKKEMKEKAIEIIGKCGLFPEQADRYPHQFSGG
                     QRQRICIARALAVNPDFVVCDEAVSALDVSIQSQIINLLKDAQEDFGLTYMFISHDLS
                     VVKFISDEIAVMYVGQIVEKGSKRQIFSNPLHPYTEALLSAAPSFDPSYRQNEKRIIL
                     RGDIPSPANPPKGCRFHTRCSYARDICKEEIPEYREINDGHYAMCHKVNGVF"
     gene            17656..17907
                     /locus_tag="PKK75_03640"
     CDS             17656..17907
                     /locus_tag="PKK75_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82719.1"
                     /translation="MFFNKKRVEEDLDRIRRANLPNKNETNNKEETENLEKSQDDIRL
                     EKGDMLAMVLAVISLVLPYILAFLGIMGIVVFLMYLFFK"
BASE COUNT         5440 a         3307 c         3594 g         5280 t
ORIGIN      
        1 tggtgcggat gagaggagtc gaacccccac ggttgcccgc tagatcctaa gtctagtgcg
       61 tctgccaatt ccgccacatc cgcatgatgc tctcagacag caaaattgat tatactactg
      121 ggaaattgct ttgtcaagga catttttgaa tgatttattt ttaaatttta ccagttcttc
      181 gaagctgtct attatgtcta tatcattaaa ggtcagggtt tgtcccgtta ttttacacaa
      241 attaactgaa tatgcaacag gcttttgata ttccttcgca taatttacca tatgcgatgt
      301 gccgcctctc aatccccctt caactattat tactccttct gaaacagctg ctataagtct
      361 gttcctgtct atgaaggaat ttttttcagc cttgtaagga ggaggcagtt cagatatgat
      421 taagcccccg ttataaataa tagtgttgta caaggacctg ttttgtttta aaacattaag
      481 gtggcctgaa ggtactactg caacggttct tccctttgcc tgaaggcatc cgtagtgagc
      541 cgcttcatca cagcctgcgg caaaaccgct tacaatacag cagttatctt cggtcagcct
      601 ctctgccagt attgaactta tttcatatcc ttcctgggac ggagttcttg tgcctactat
      661 tgctatgttg tttctatcat tcaaaagctc caggtcgccg tccgcatata atatcagtgg
      721 cttgtcttct attgatctga atttgtatgg ataggtttta tcatatacac cgataatttt
      781 aattttattt tttttgcagc attcaaaaat tactgctgct tccctttcga ttttctcttc
      841 atcaattttt agccttttga gattcatttg gctaatcatt tttaagactt cagaaaagtt
      901 gttcagcctt atgtttttaa attctatgta agataatatt ttactgatag tttttcttcc
      961 caatccgtta atcaggtgca gtctgattaa gtcctcgttg cttatcatct ttctcccttt
     1021 gaatggtaat gaattaatca taccctgaga ttaatattac ctattataat acatcactac
     1081 gtaatattgt gttgtttttt agaataaacg aaaggtgtgt ttttacattt taccacaaaa
     1141 ttctacaaaa agaactattc ttttattcag aatagttaag aatatttaat atatttatat
     1201 tgtgtacata tatttaatat aaatataagg ggtgggttca tgaatagaag aagaaatatg
     1261 ccgtatatcg cacggaataa aataaaaaga gctaaaaagc ctgaaatact gcaattatta
     1321 agcgaagcag agcttgacgg agacaactcc aatatccaga caggttttgt cagtgtatct
     1381 gtaaacacag ctttgggtgc cgttcctgtc agggatgccg ttgttaccct atatgtgaca
     1441 gacctggaag gtaacgaaga agccttgtac catctggtga cggacataag cggaaaggtt
     1501 cccaagatgg ctcttccggt ggtatataac ccccaggacc cgctgggaag ccgggcgttt
     1561 tttttctcaa catataatct aagaatacag gcaatcggtt attatacttc aaatattata
     1621 gacttaaggg tttttcccga cacgacaact aattttactg tgacgctgat acctgtcagt
     1681 gagggcggag atgaagatga aggagaacgg accatagtaa taccttcaag ccctattgat
     1741 gaatccaatg tgtgaggtgt taaaatgagt gtaacaacaa tccagcagac atttcccgaa
     1801 tcaccgctta agcttggtga tgacagccag tatgtactta ttgcaaaaga ggcattaaat
     1861 gtaatatctg taaactatcc tgccatacca gtgctggtag ccgttaatac agtatttgat
     1921 gaaaatatgg aagctgcagt gaaagaattt caaaatatat ttgatcttcc ggtaacaggt
     1981 ataatcgata tggctacatg gtttcaaata ggaaccgtac aagcaactgt gctaaggctt
     2041 gcagagcttg caggcagagg agttttagtg ggtgatacgg ttgaagatat cacggaagta
     2101 gaagaggggg ttcaggttct tccaagggtt caaatggttc agtttttttt aaacgttttg
     2161 tcggcttttt atgattcaat aaatgcagtt gatatcaacg gcattttggg acctgaaaca
     2221 cgaaacggca taagagaatt tcaaaaaacc atggggcttc caacaacagg aataatcgat
     2281 gaggaaacat ggattgcaat gtataacagc attcttggaa ttttaagaga gttgccgcct
     2341 gtggccgttc agctgccttc cctgctttat acaggcaccg tatacagcga aggctcggaa
     2401 aggccgggag tattaataat acaagagata ttagcatata tttcaactgt aaattccgat
     2461 ataccctttg ttcagccgga tggtatattt ggtcccgaaa caagggcttc cgtatcagcc
     2521 tttcaaagat actacggtct tgaggaagac ggaattgtgg acgaagaaac atggaacaaa
     2581 ttaattagtg tttacaggga attgagattc ggtgatacaa atctacttcc tgatgcaggc
     2641 gaaagcttga tacaataatg aaaacaaggt gataatatga gtattgataa tgtaaatgta
     2701 tttattcccg aggctggcct tccccatgtg attgtgcctt ttccccttta tataaccgtt
     2761 catttgggac ctcccgacga agatgcactg aatgtaactg ttccatattt ggattatatt
     2821 aaaaatgtgg gctcaagtga attgtatccc acctggccgg aagaagcttt aagggcaaat
     2881 attaatgcaa tttcttctat tgcaatgaat agaatcttta cggaatggta caggtcacaa
     2941 ggttatgaat ttgatattac aaataccacc cagtacgacc aggcctatgt tcacgagagg
     3001 ggtattttcg actccatctc caatattgca aatgaggaat tcaataggta tattgtcaga
     3061 aatggcttca ttgagccaat gtttgcaacc ttttgcgacg ggagagtttc tgtgtgcgac
     3121 gggatgcatc aatggggttc tgttagtttg gctaatcaag gttatacatc agaagatatt
     3181 ctcaggtatt actacggcga tgatataact ttagtcgata gtacaactgc agttgtgatt
     3241 acaggaacct atcccggtga gcccctttca ctaggcgatt caggaataga tgtattcaga
     3301 atgcagcatt cactgaggaa tataagcgac agttttccct tgattccccg tgttgaaata
     3361 acaggttttt tcgatgaaga aactgaaaat gctgtaaaag tatttcaaga agtatttggc
     3421 ctacctgtaa caggagtagt agacagtcaa acatggtacc ggatccgctt tacgtatatt
     3481 gcagttgaaa gactagccga gctggtatct gaggggcttt cctatgaaga attaagacag
     3541 ctttattcaa atattgtact ggagggggca accttgccca tagtggctta tattgaattt
     3601 tttctaaatg taatatccac aaaatacgat acgatagagc ctgtggaaat agatactttt
     3661 tatggtcccg atactgcccg ggcggtaaga gaatatcaaa ttataatggg tttaaaccct
     3721 acgggaattg cagatcagga aacattgaat ttattgtaca gggatgcata ttcagttctt
     3781 acatcaacac ctgtagaaga attgacgctg ccctttcttc cttacacggg agtggactta
     3841 actcagggta tgggccccga gtatccaagg attgtgcttc ttgaaataat gctgaatact
     3901 atttctctaa cgcagcccga aataaggcct gttgaagtag gcggcgggtt tgattccaat
     3961 acaagagctg cagtgatagc ttttcaaagt ttgtacggtc tgcctcctac gggtgttgta
     4021 gatgaagaaa catggaatag gataaacgag gtgtaccagt ctgttatcgc aagtaataca
     4081 acaggttcat aacaaaaagg agggattatg gtaaatttag aaaataacac taattttatt
     4141 ctggaaatcc aaaactatct taataatgtg aatcatgcct ggcatatgat agaacttaca
     4201 ggagtatatg accaggcaac ccggaatgct gttcttatat ttcaaaatgc aaacgggctt
     4261 ccccccaccg gttatgttga tgtccccaca tgggctgtgc tggcaagtaa aaataatgaa
     4321 tatcttagaa aaactcaaat gccgggaaaa attaccgtca gtaatcctga atttaaagat
     4381 gtgaaaacag gtgaccaaaa tgatttagtt tatggaatta aggtcatgtt taacaacttt
     4441 tccagaagat atgcaaatta tccggaattg gaggtaacaa acctatatga tgaaaaaacc
     4501 gaagaagcag ttatactttt tcagcaaaga agcatgcttc cagtaacagg aattgttgat
     4561 ttgaaaacat ggaacagctt ttgtaaaata tatgaggtct gcagattgta cgaagaacga
     4621 cattaagagt cacaggcggt accatcagct tgtgatagta ttttatattt cggggagttt
     4681 ctcctttttt tatttaacct attgacaaat actgtggttt tgataaaata tgtttagcac
     4741 tcaacgcttt agagtgctaa caaccctatt gttaataata aataacctga cataatattt
     4801 tagattttgg cttctcccgg atatggctgg agctataaaa gaagaataaa agtcagaaaa
     4861 acataagata tgaaaggaag ataaaatggc taaaaaacaa tttaaatcag agtccaagcg
     4921 gcttttgaac cttatgatca attccattta cacacacaag gaaatatttt tacgggagct
     4981 tatttcaaat gccagtgatg caattgacaa aagctacttt ttgtctttaa cagacaaaaa
     5041 catccccttt aacaaagatg acttctatat aagaattaca acaaatgaag aagaaagaac
     5101 attgaccgta tctgacacag gaattggaat gtcaaaggat gagcttgatt ccaacctggg
     5161 aattatagca aaaagcgggt cctttgattt taaggaagac atcgaaataa aagaaagcgt
     5221 cgatataata gggcagtttg gagtgggatt ttattctgca ttcatggtct cagataaggt
     5281 aaccgtaata agcaaaaaag cagattctga tgaagctttc aaatgggagt caacgggagc
     5341 agacggatat acaatcacac cctgcagtaa agaaacagct ggaacagaca tcatcatgaa
     5401 aatcaagcca agcactgaag acgaagatta cgatgagttc cttgattctt acaatattaa
     5461 atccctggtt aaaaaatatt ccgatttcat caaatatcct gtaaagatga tgataaaaaa
     5521 aagaaaactc aaggatggca gcaaaaatga atacgaggaa tacttcgaag atgaaatact
     5581 aaacagcatg gttcccatat ggagaaaaaa taaaagcgag cttaaagaag aagattatga
     5641 aaatttctac atggaaaaac atttcggcta tgaaaaacct ttgaagcata ctcatgtaag
     5701 cgtagaaggg gtaatcagct acaatgcaat attgtacata cccgcaagag cgccctttga
     5761 tttctataca aaggaatttg aaaagggtct tgagctttat tcaaacgggg taatgattat
     5821 gaaaaaatgc ggtgagctgc tgcctgatta tttcggtttt gtgcagggcc tggttgattc
     5881 tcctgatttt tccctcaata tttcaaggga agtattgcag catgacaagc agcttcaatt
     5941 tatcgccaag aaactgaagg aaaaaatcaa aagcgagctt tttgccatgt taaaggatga
     6001 aagagaaagc tacgaaaaat tcttccaaag cttcggcaga accttgaagt acggcttgta
     6061 cagcgaatgg ggttcaaaca aggaaacact tcaggacctg gtaatgtttt attcatctca
     6121 cgagaaaaaa ttggcaactc tcgatgagta tgtaagccgg atgaaggaag accaaaagta
     6181 tatctattat gcggctggag aaagtgttga taaactggcc aggcttcccc aagctgagct
     6241 tgttgcggac aagggatatg aaattctgta ttttatagat gaaatagatg aatttgccat
     6301 aaaagtactc tttagttaca aggaaaagga atttaagaat atttcaagtt ccgacctgga
     6361 tatcaaggcg gaggatgata atactgctga tgctgaagaa aacaaggaaa tatttgcttt
     6421 catgaaggaa gccttaggtg acagggttaa ggaagtgagg tcttccaaga ggctaaagac
     6481 tcaccccgtg tgcctatcag ccgaaggaga tgtatctatt gaaatggaaa aagtcttaaa
     6541 tgcaatgcct gataatcctg cagacatccg tgcggagaag atacttgaaa ttaacaccgg
     6601 ccacaaggtt tttgaaaaac taaaggatgc ttatgacaat gacagggaaa aacttaaaaa
     6661 actgtcaaat gtattatata atcaagcact gcttattgaa ggactcacca tcgaagaccc
     6721 tgtacagtat gcaaatgatg tgtttgaatt gatgtaagat gggccaacct cattttgaga
     6781 tgtcaaagaa gccgcgggaa tttgctcccg cggcttcttt tattggaagt attcttccat
     6841 agtgagtcct gattcatata acttttctgc aaggtcttca cccaaatatc taatatgcca
     6901 gggctcgtat tcgtagccgg taatcttttc cttgcccttt ggatatctta ttataaatcc
     6961 gtaattgtgg gcattctctg caacccactt tccctcttcc gtttctgcaa atgccgtatc
     7021 caatttataa tttacagcct cgcatgtaat atctattgaa agacctgtct gatgttcgct
     7081 ttgaccgggt ttggcgctgt acttgtctgc agcagccttg ccgtagctgt taacatagga
     7141 tgaatacagt gatgactggg tataatatga cctgtagcct gaccgcgcat ggagttcatt
     7201 gcctgtctct gaagcagcag tgaacatttc cttcaatgct tcataggcgg cgcttctcaa
     7261 ttgatttacc tcagggtttg aaagtactgt aggcacttcg ccgagtgtta ccaaatcctc
     7321 aggtacatag ttttcaggca ggtttctttt tctgttaacc agcacatcca gcttttcagg
     7381 gttctcgatt gtaatgccgg gttcatcatc gggttgctcc tgcaccgggt cttcttcttt
     7441 aggctcaggt ttctcgggtt ccggtggctc ttgggaatct tccggctctt cttcctgagg
     7501 gttttcttcc tgagggtttt cttcctgcgg gttttcgggt tcaacggaag tttcctcttc
     7561 ttccgggttg tcaggcaggt ttgaaggctc ctccccccta tttcctaatt tctctattat
     7621 gagaaaggcc ccgaacaata ttaacaaaag tgcggtaaca acaattatgg tccttcttat
     7681 ccttagtttt ttatttcttc tcattatttt ctcctagctg tcaacctcnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ccatccacat tatcagtgca tcctccaagg
     7861 gcttaacata ataatttggt ctcttcccaa cggcataaaa tccaaacttc ttatataaat
     7921 tgattgccgg ttcatttgat atcctgactt cgagagtcat gtaagaaatc tcagagcttt
     7981 tgcatatttc aatcatctta tttatgagca tgcctgctat tcctttgttt ctgtattcag
     8041 gaagaacggc aacattggtt atgtgacatt catcaataat tttccacatc cccatatatg
     8101 ctgcaatttc ccccgaaact tcagccacct ggtaatatgc caattcattg gttagttcat
     8161 tttcaagaga tgccttagac cagggcattg aaaaacaaat tctttcaata ttcataacct
     8221 ggtcaatatc tttttgaagc attcctcgaa ctattacatc ccccatacac acctcttact
     8281 ttctgttcct ttctgcctgc gacaagcgga gataccgggg cttaaattca tctgccgtga
     8341 cagtatcccc tgcaaccgcc attttataag caataaagcc tagtgttgaa gctttgagat
     8401 aattgcaggc ttcaggtgca aaatatgatt ttctgtttaa attattaagt attctttcca
     8461 tgtaatttac tgatccgtct ccgttaaata caaccggttc ctcatattta tttaatattt
     8521 ggatcaattc gtcaatattg caggcaaact gctcctccgt ttcccttaat tccctgttat
     8581 cccacctgta aatgccggta tatattcttc cggcctttgc atccattaca ggtacgatta
     8641 atttatcctc atcataaata ttggctgcca gtgatttcat tgtaggaaca cctattatcc
     8701 ttaaattccc ggcaaatgcc aggcttttgg caagagcagc ccctattctg agtcccgtgt
     8761 atgagccggg gccttcagaa actgcaagga aatcaatgtc cttaagcttt agtcccagat
     8821 cctccatcaa ttcctctatc aaaggcatga gcttttctga atgagttttc ttatgactta
     8881 aggtatattc acctaaaaga ttttcgtctt cggatactgc acatgaagct gccacggatg
     8941 ctgattcaat tgaaagtatt ttcatttttt aactccctga ccagtttttc gtaaaccggt
     9001 ccttttcctt caatgtttat ttcccttttc gtatcatcaa ttctaataat cttaatatta
     9061 actctttctt ccggaagcat tccccggatt aaatcagccc actcaattac tgtaactccc
     9121 tcagaatata tatattcatc gaatcctaaa tcatacatat catcttccga acccatgcgg
     9181 taggcgtcta tgtgaaaaaa tggcagtttt ccgtcatatt ctttaatgat tgtaaatgta
     9241 ggacttacca tgtattcttt cacatcgaat tcttttgcta taaactgagt aagagaggtt
     9301 ttccctgctc ccagttctcc gtcaagacaa atcacggcac ccttttccag gcattttgct
     9361 attatttttc ctaatttttc ggtgtctttt aaattgtttg ctgtaatttt catattattc
     9421 ctgacttcat ttttttcttt cataacatcg ctattataca tttatgtatt aaaaaaatca
     9481 agaaataatg aaccaaataa accggtgatt gagactgcat aaaatcatcc ttaaaaccca
     9541 agaacctgaa gcttcccctg gatggcaacc cgccgggcat gaacagcaag ctagggacag
     9601 ccgcccagga cagccggtga aaaatttatt gaacaatggg tgtgcacgtg ctataatact
     9661 atgagttatt tatacattta gtaaatataa taaaaaaatt tatatcaaga gtgttaattg
     9721 cctgctaaat gaataatata aaactataat gaatacccgg ggagattaaa atgaaataca
     9781 acttcaatca tatatatttt ataggaatcg gtggaataag catgagtgca ctggctgaaa
     9841 taatgcttga ggaagggatc aaggtttcag gctctgacag aagcgattcc aaaataataa
     9901 aaaaccttca aaaatcaggt gcagatattt acataaagca tgacagcaaa aatataggtg
     9961 atgacatcga cctggttgta tatacatctg caatcgctga ggacaatccc gaacttttaa
    10021 gagctaaaga actgaatatt aagataatgg acagggctga attccttggg gcagtaatga
    10081 aaaattacag ccatgctatt tgtgtttccg gcacccatgg caaaacaacc actaccggaa
    10141 tgctttcatc tgttttaatt gatactgatt tagctcccac tatttttata gggggtgaaa
    10201 tggattcatt gggaggcaac ctgcttcacg gctcatacga tcttcttttg actgaagcct
    10261 gcgaatacaa aagaaatttc ctgaaattta acccgaccat ggaagtgatt cttaatgttg
    10321 aggaagacca tcttgattac tacaaggatt taaaagatat tgaaagtgca tttgttgaat
    10381 atgctgaaaa actgcctcct gacggtcatt tgattgtcaa tgaagcatac agtcatttgt
    10441 tcaataatat aaaatgcaag gttgtgacct tcggccttga taaaggcgat tattatgccg
    10501 ggaatacaag gcttatacct gaaccctcat atacactgat gaacggggat aaggaagtta
    10561 tagaaatcaa tcttaaagta ttcggcgagc ataatatttt aaattcattg gctgcatgtg
    10621 ctgcatgcct gtctttgggg attgagcctg aaactattgc caaaggcttg aatgaattta
    10681 aaggcactca cagaagatat gaatataagg gcgagattaa cggggcagtt attattgacg
    10741 actatgccca tcatccttca gaaatgaaag ccactttaaa aactgccagg tcttatgccg
    10801 gtggcagaat aatcactgtt tttcagcccc atacctacac cagaacaata aagcttctgg
    10861 atgggtttgc tgaagcttta tccatgtctg atgaggcaat tttagtagat atttatgctg
    10921 caagggaaat tgacaagggt gagattcatt caatggatat aattaataaa atgaagaatt
    10981 attccaagaa tggaatctat gcggaaaact tcctggaagc agctgatata gtacggtcaa
    11041 tggcaaaaaa aggtgatatt attatcacca tgggggcagg aaatgtagtt gatatagggg
    11101 agatgctgct tgagtaggtc aaataatcat ctcgcagtcc cgttcaccaa aatataataa
    11161 tgtacatctt ctaacaagca gaagttttct gggaaccgag atgtatgtcc ttttaaattt
    11221 aaatcatctt tccctgtttg taagaaattt tgccagttca acaaggtatt cagctctgcc
    11281 gccgaacatg ctcaagctct caattgcttc ctgagtgaat ttttcaacgt cttgccttgc
    11341 ttcttctatg cttttaaatg taagatatgt aattttctta tttgcagcat cacttcccac
    11401 tgctttgcct aatttttccg gggtgcctgt aacatccaag atatcatctt ctatttgaaa
    11461 tcctatgcct attttttcag cgtaggatcc cagttgtctc atttcttcct catctgcgcc
    11521 tgccaattga gctcctgcaa ttacgctgct tttgatgatg gcccctgttt tcaaagaata
    11581 catatatttc aggtcttcaa tggaattgat tatttcatcc ccttccatat ctacaacctg
    11641 acctccaatc attccttccg tacctgaaga gtcggcaatt attttcaggg cttttacggc
    11701 gttcatgctt ttcgaattta ttgaagcttc caatcctgtt tcaaaggcct tgttaagcag
    11761 accgtcgccg gcaagtattg ctgttgcctg gccgtacttt ttatggtttg acagcttgcc
    11821 tcttctgtaa tcatcattgt ccatagcagg caaatcatca tgaatcaatg agtatgtatg
    11881 aatcatttcc attgcacagg caaatggcag ggcttctttt aagtcatccc tgaacagttc
    11941 ataggctcct aacaaaagca caggcctcaa ttttttccct tctgataaaa ggctgtaatt
    12001 catggcttca tagattattt tttgaggatt ttccctttct tcgataatcc ggcttagata
    12061 ttcgtcaaca atttttactt tttctttaaa ccatgttata aattccatgt taatctttcc
    12121 tttgttctat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaagatatt
    12241 atcactgctt ttagctgtaa tgatgattgc tgtggtattt gcaggctgta cacaagcacc
    12301 gccggctgaa gaaccgccgg cagaagaacc accggctgaa gagccgccgg ctgaagagcc
    12361 aaagggtgac caaatattga agtttggcgc aaccggattt gacggagtat ttaacccgat
    12421 tttgtctgat aatgtgtatg atgcttatat cgaatccatc atatttgaaa agttgataac
    12481 aaataatatt gaaggcgaat atatacctaa tatcgctgaa tgggtgttgt cagaagacaa
    12541 gctgacttac acattcactt taaaagatgg aatcaaattc tccgatggtt ctgatttaac
    12601 tgcagaagac gttgctttta cctacatgac catcgcacat ccggactaca atggacctag
    12661 agcatatgct gtaaacaact ttgtaggcta tgatgaatac cacgcaggag aaattgatgt
    12721 atttgaagga attaaggtta ttgatgaaaa aaccatatcc tttacattta aggaaggtat
    12781 ggcagcacct gcaaatattg aatgctttgt atatggcata atgccttcag actattatgc
    12841 atttgacaca tgggaagatt tcctggcatt taatgacaaa ccaatgggtt caggcataat
    12901 ggtttttgac agctgggcac cgaatgaatt catattgttg aagaataaca caaattattg
    12961 ggatcctgac aatgcagcta agattgaagg agttctggta agtgaagttc ctgatgaaag
    13021 tatactatct gctttgcaga cagatcaaat agatttcgca caaatagctg ccaccggtga
    13081 aaaccttgca gcagcagaag ctatggataa tatccaggtt gcaaattatc ttgcaaacgg
    13141 ctatacattc atgtgcttta acacaacaag accccaatta tcagatgcaa gagtcagaca
    13201 agcgcttatg tatgcccttg acagagagtc atttatagat gttcaatacg gtgaaggctt
    13261 aggttcagta ggtatggctc caatttctcc ttcatcatgg gcattcccgg atccatcaga
    13321 gttaaaccca tatgcatttg atatggaaaa agcaggacag cttatggatg aagcaggatg
    13381 gataatgggc tcagacggat acagatataa agacggtcaa aaatttgctg ttaactggct
    13441 ggtttacaca gattccgcat ggccgggaac actgtccgga atggctgcag atacatggaa
    13501 acagttaggc gttgaactta caatagaatt aatggacttc aatacggttg cagctcgtac
    13561 aatggatgca gagccgggtg aaaaagattt tgatatttac acaatgggct tctccttgag
    13621 tatagatccg gacccaacag gagcattgtt tgatgatgat gcttatgtag caggcgggtt
    13681 taacgcatca ggttacaaga atcctgaagc aatggaactg gtaagacaag gaaaagcaga
    13741 atttgacaca gcaaaacgtg ctgaaatcta caaagaatgg gcaaaaatta tgaatgcaga
    13801 aattcctcat gtaatcatcg cttacagaag cgaaatctgg ggaatcaaca atagagtaga
    13861 tggaatggta attgacacct acgcaacttg ggatcaatcc ttaaagaacg ttactttaaa
    13921 atagcaacag gggcaggcag cgcctgccct tatttaatag acccttaaag ataaacatta
    13981 agaaagaggt gttttatgag gaattatatt ataagacgta taattcagat gattcctgtt
    14041 tttataggaa ttttatttat attgtttttt attctcgagc aggcaccggg aacaccggtt
    14101 tcaaatatga tgcacccaag gatgactcct gaacaaaaag cagaattaaa tgagaagttg
    14161 ggcatggata tcccctggta tgaaagattt gtgaattatg taaaggaggc gctgttagaa
    14221 ggcaacttag gttattccaa cactcacaaa agacccgtag ctgaagttat cggagattat
    14281 gtaggtccta cattgcttct ggctgttgtt tccttatgta tttctattat tatcggcata
    14341 cctgccggca tcttaagcgc aacaaaacag tacaccgctg ttgacaacat attgactgtg
    14401 gtatccttta taggtataag cataccttca tttttcttcg gtctgatact gttgaaggtt
    14461 tttgcggtgg acaaccaaat attccctttg tttggcataa gagacccctt attaatgtca
    14521 gacaatattt ttgataatat taaggatgtg gcatggcacc tggttttgcc cagcattgtc
    14581 ttaggcttaa gctctaccgc aagctttatg cgatacaccc ggtcaagcat gctcgaagtt
    14641 attaagcagg attacatcag gaccgcaagg gctaaagggc ttaaagaaaa aaccgttata
    14701 tacaggcatg cattcagaaa cggcataatc ccaattatta cccttcttgg tttttggata
    14761 ccgtcacttt tatcgggagc ggtagttacg gaaacaattt ttggattgcc ggggcttggt
    14821 aaaatatccg tcgaagctgc aatgaaaaga aactatcccc tcattcttgg aataaattca
    14881 atgcttgcaa ttcttacact gataggcgcc ttagttgctg acatcctgta tgcagtcgca
    14941 gacccgagaa taagatacga ttaaggcagg tgcaatatgg ctaaagataa aactaataaa
    15001 aattatatca gaaatcaatc ctattgggga gaagtatttt tcagactcag aaaaaacaaa
    15061 atggcgatgc tgagtcttta cattcttgtt gcaataattt tactatgtat tattattcca
    15121 atcatatcgc cttatgagat tcagacaacc aatctggcag gcaaagacca aagtccaaat
    15181 tccaagcatc tccttggaac agacttaatc ggaagagact tgtttacaag gcttttttat
    15241 gcaggaagaa tttccttagg ggtagccctt gcagtagtgg ccatcgaatg cgtaataggt
    15301 gttgttttag gaagtttgtc cggtttttac ggaggaatta tagatggggt aattatgcgt
    15361 attgctgaaa tatttatgtc atttcctttc atgattatta ccattacaat agtggctgta
    15421 ttcgggaaca gtatttttac cttgattttg gtactggctt tactaagctg gcccagtatc
    15481 gcccgtattg ttagaggaca aatactatct cttagggaaa tggaatacat ggaagcttgt
    15541 gaagctctgg gaatcagcga cataagaaga atattcagac annnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn ntgctatgag caaagaatta ctaagagtta ataacctgaa
    15721 aacatatttt tattcagaca gaggaattgt aaaggcagtt gatgacgtaa gcttttacgt
    15781 taatgaaggt gaaactttag gtattgtcgg cgaatcaggt tgcggaaaga gcattacctg
    15841 tatgtctctg gtaagattag tggaaacacc tccgggaaaa tatgagggcg gtgaaataat
    15901 ttttaacggc gaggatatgc ttaaggtttc agatacaaga ataagacaaa taagaggaaa
    15961 tgacatatcc tttatttttc aggagcctat gacttcatta aatcccgtgt tcaagatagg
    16021 gagacaaata agcgaggctt tgatattgca tcgtggaatg tcaaaggatg aagctcgcaa
    16081 ggaaagcatc cggatgctgg agcttgtaaa aataccaaat ccagaaaggg tggttgatga
    16141 ctatcccttt gcattatccg gaggtatgag gcagagggca atgattgcca tggctcttgc
    16201 ctgcgaaccc atgctcctga ttgctgatga gcctactaca gccttggatg taacaattca
    16261 ggcccaggtt ctggatttaa tgaatgaact taagaaaaaa attaatgctt caattatttt
    16321 tattacccat gaccttggtg taattgcgga aatgagtgac agggttatgg ttatgtatgc
    16381 aggcaagatt gcggaaatgg cctcaacagt ggaaatattc aaaaacccca agcaccctta
    16441 tacgatgggg cttataggtt caaaacctga tatggcaaca gaaagcgcaa gacttaacgt
    16501 aatccccggc aatgtgcctg atttaagcaa ccttccagca ggatgccctt tccatccaag
    16561 atgtgacaag gcaatggata tatgcaaaaa agattttcct gccgaaaaaa cactttatga
    16621 cggacataaa atttcatgct ggctttacac aggcaaggag gaattacaat gagtgaatat
    16681 ctgttaaccg taaacaattt aaaaaagtat tttcctatta aaacaggtct tgtaaagcgt
    16741 accacaggaa atgtaaaagc agtagacgga gtatctttca atattaaaaa aggcaaggtt
    16801 ctgggaattg taggagaatc aggctgcgga aaatctacct tgggaagaac tatcttaaga
    16861 ttgctgacac ctacagaagg tgaagttttt tatgaaggaa aaagcgtttt tgacatggac
    16921 cacaaagaaa tgaataaaat gcgcaccaaa atgcagataa tatttcagga tccctattct
    16981 tccttgaatc cgaggcttcc tgtttatgat atagtaggcg aagcaatgct tcagcatagt
    17041 attgtaaaga ataaaaagga aatgaaagaa aaagcaattg aaataatcgg caagtgcgga
    17101 ctcttccctg agcaggcaga ccgttaccct catcagtttt ccggaggaca gcgccagcgt
    17161 atctgcattg caagagcttt ggctgttaat cctgattttg tagtatgtga tgaagcagta
    17221 tccgcactgg atgtttccat acaatcccag attataaatc tgttaaaaga cgctcaggag
    17281 gattttggac ttacttatat gtttatatcc cacgacctgt cggtagttaa atttataagt
    17341 gatgagatag cagttatgta cgttgggcag attgttgaaa aaggaagcaa aagacaaata
    17401 ttctcaaatc cccttcatcc gtatacagaa gctctgttat cagctgcccc ttcctttgat
    17461 ccgtcttata ggcaaaatga aaaaaggata atattaaggg gtgatatccc ttcaccggca
    17521 aatcctccaa aaggatgccg tttccataca aggtgctctt atgcaagaga tatatgcaag
    17581 gaagaaatac ctgaatatag agaaataaat gacggacatt atgctatgtg ccacaaggta
    17641 aatggagtat tctaaatgtt ttttaacaaa aaaagggtag aagaagattt ggatagaatt
    17701 cgtcgagcca atctgccaaa taaaaatgaa acaaataata aagaagaaac agaaaattta
    17761 gagaaaagcc aggatgacat ccgtttggaa aaaggcgata tgctggctat ggtgctggcg
    17821 gtcatatccc tggttctgcc ctatatactt gcctttttag gaattatggg tatagttgta
    17881 tttttaatgt atttgttttt taagtaaagt attaatccgc a
//
LOCUS       DAOPUT010000023        17432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3315_length_17432_cov_17.748058, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000023 DAOPUT010000000
VERSION     DAOPUT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 17432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17432
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3315_length_17432_cov_17.748058"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PKK75_03645"
     CDS             <1..257
                     /locus_tag="PKK75_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ82720.1"
                     /translation="GITCNMICPSYVRTPLVDKQIADQAKTHGIPEDKVISDIMLTKA
                     FVKKLLEPKDVGEFAKFLCSPTGDYITGAMLTIDCGWTAS"
     gene            316..1602
                     /locus_tag="PKK75_03650"
     CDS             316..1602
                     /locus_tag="PKK75_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HNZ82721.1"
                     /translation="MFGIILGLVLLMFLAYRGYSILWVAPVCALVVGLTGGLDLLTMY
                     KDTYMTGLSGYVKSWFPVFMLGAIFGHLMDYTGSAKSIARWLTKKLGPKRAILGVVVG
                     CGVLTYGGISLFVVVFAMYPLALELFKEANITRKLIPGAIALGSFTFTMTAIPGTPQI
                     QNLIPMNYYGTTPTAAPIMGIVAAVLMFGLGVFWLQYREKKFTAAGDVYTEPKNREGI
                     EVDYSALPNPFISFLPLITVIVTMNVVDWDIIYALLSGIILSMAINFGKYKGFTKTIS
                     NGALGSMTAILNTAAAVGFGTVVKAVPGFASLTAVLLNVPGTPLISEAIAVNLLAGAT
                     GSASGGMSIALEALGEKYMQIALETGISPEAFHRVASLASGGLDTLPHNGAVLTLLAV
                     TGLTHKDSYIDVMMTSLVLPIIASIPAIILGSLGIY"
     gene            1826..2782
                     /locus_tag="PKK75_03655"
     CDS             1826..2782
                     /locus_tag="PKK75_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNZ82722.1"
                     /translation="MKLQDIKKIGVAGGGTMGSGIAQIFAQNGYEVAVTDIAEKFLEN
                     TKRIILLNQKTLIKEGLLTQEQAEESLKLITYSTDNGVFVDADLIVEAIIEKMDIKQD
                     YWKEVEGIAPNSCIFATNTSGLSINGICSKLNNKKRFIGMHWWNPPHIIPLIELIKND
                     ETSDETVRILKELVDKIGKESVVVLKDVNGFIGNRIQFAVYREVLKIIEDGVATIEDV
                     DKAMKFGPGFRYPVLGPFETSDLGGLDTFYYISSYLFNDLSDVKKPTKLQQEKMDNGH
                     LGVKTGKGWYDYSDGKDVETMTRRDENFFRMYKSYITGKYKK"
     gene            complement(2839..3930)
                     /gene="ychF"
                     /locus_tag="PKK75_03660"
     CDS             complement(2839..3930)
                     /gene="ychF"
                     /locus_tag="PKK75_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNZ82723.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITSAGADSANYPFCTIEPNIGVVEV
                     PDNRLDFLTKMSNSARTVPAVIEFFDIAGLVRGASKGEGLGNKFLSHIREVEAVVHVV
                     RCFEDENVIHVETTVDPERDIEIIELELILADLEVMSKRLDKLSKTVKNDKSLQPEFE
                     LVKKITEGLEKEKQARYLTYTDEELKILKTFNLITSKPVLYVANVDESYLSNPSDNRF
                     VDIVKKYASKDNSEVIVICGKIEEEISVLEDDEKKEFLMEMGLAESGLDQLIRISYRL
                     LGLISFFTTGPMESKAWTIVKGTKAPQAAGKIHSDIERGFIRAEVTKYDDVFNLGTMQ
                     AAKEKGLMRLEGKEYVMEDGDIVLFRFNV"
     gene            4140..4817
                     /locus_tag="PKK75_03665"
     CDS             4140..4817
                     /locus_tag="PKK75_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ82724.1"
                     /translation="MNDKQMQSNYIKIVGIIFSELEFSHEVFGEKFFEFFIEVPRLSE
                     TKDTLPVIISERLINDINMDIGNYIVIEGQFRSYNRYEDAQNKLLLRVFVRDIYVPDY
                     TELEEMKRHPNEVFLNGYLCKETKFRTTPFGREITDMLIAVNRSYNKSDYIPCIAWGR
                     NARYCEKLEVGDHIKIWGRIQSRRYQKKNENESYDTKTAYEVSVTKLEHIKTENNRKK
                     EDEDMEL"
     gene            4923..7100
                     /locus_tag="PKK75_03670"
     CDS             4923..7100
                     /locus_tag="PKK75_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HNZ82725.1"
                     /translation="MIMILVLAEKPSVGKDLGRVLNCSKSGNGFVEGSIYIVTWALGH
                     LVTLADPEKYNPKYERWELEDLPIIPKRMETEVIKQTRKQYNLVKELMHRNDVKEIVI
                     ATDAGREGELVARWIIEKAAVNKPLKRLWISSVTDKAIKEGFNNLKDGKAYETLYASA
                     LARSEADWIVGINATRALTTKYNAQLSCGRVQTPTVAMIAALEDEIRDFRPVSYYGIE
                     AAAGSIKFTWNENNDTKIFDESKCDAIIDSIKGSKLKLTDITRKKALSYSPLLYDLTE
                     LQRDANNIFGFTAKETLSAMQTLYERHKVLTYPRTDSRYLTDDIVETLKDRVKAASVG
                     PYSRIGFQISQNPIKASKNFVNNSKVSDHHAIIPTEQTVILSELTDRERKIYDLVVKR
                     FFAVLSKPFEYEKICVTGIINNEEFISKGRITLSMGWKEVYGNYEDEEILDEYPVEEL
                     NKLKTGSELSVSKVYKTSGETKPPSRFTEAALLSAMENPVKFMKNADKDLKRTIGETG
                     GLGTVATRADIIDKLFNSFVIEKKDKYIYTTSKGRQLLDLAPADLKSPVLTAQWEQKL
                     NEIARGNLDKNKFLNDMINYTKTIIKEIKSSNKSYIHDNETREKCPDCQEYLLEVKGK
                     KGTMLVCKNRECGYRRGISQITNARCPDCHKKLELKGEGEGRLFVCKCGYREKLSTFN
                     KRKANEKGSITNKEASGYMYKQNKKQDESINTALADALKKLNF"
     gene            7115..7756
                     /locus_tag="PKK75_03675"
     CDS             7115..7756
                     /locus_tag="PKK75_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82726.1"
                     /translation="MHYVIIGLFAVYIIYLFIRRNSLKIQVEQFNKLLYADKKPDQYI
                     QEIDNLLAKRQSKTEIDINYIQKSTGLLYAGRFDEAAGILTEKVNKIPNFWQVVYYHN
                     LLLSLYFGGSTEKANGILNEVKDVMDSYYKKDYNKVTIELINAVSDYYNDRISESRDF
                     FNKLPDITSNEYRKAMGYYYTGKILENEGKKEESNELLEKAKTCAQGNFIEYL"
     gene            7766..8629
                     /locus_tag="PKK75_03680"
     CDS             7766..8629
                     /locus_tag="PKK75_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006438970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNZ82727.1"
                     /translation="MDIVQMDNKITIHDVKDFNTRHIFECGQCFRWNKEEDGSYTGVV
                     KNNVINLRQEGSTVEFNNISDYDIIRSYFDFDTDYAVIKKALNTDEIMKNAINYGEGI
                     RILAQDEWETMISFMISANNRIPMIKKVIENLCTCFGEYICNYRGRDYYSFPSAESLS
                     QAPVEEIFQCKAGFRSPRIKEAAARFLLERDQIYNIRNMDYEDGLAYLKTYKGIGDKV
                     ANCILLFSTRHFDTFPVDVWVRRIMQTLYLSKDTKDVDIRKFAEEKFGKYSGFAQQYL
                     FFYARENLIGK"
     gene            8771..10231
                     /locus_tag="PKK75_03685"
     CDS             8771..10231
                     /locus_tag="PKK75_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003337347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNZ82728.1"
                     /translation="MTNTKTKTGWNLENSYAMLPDFFYSKLNLSRVSSPKLVVLNNEL
                     AKYLGFDINELISAEGLSYLSGNDVPEGSIPIAQAYAGHQFGYFTMLGDGRAMLLGEQ
                     KIPEGKKYDIQLKGSGKTPYSRGGDGRAALGPMLREYIVSEAMHALNIPTSRSLAVVT
                     TGKPVIREKLHKGSILTRVAGSHIRVGTFQYAAYFGTDENIRQLADYTIDRHFKEIEK
                     NDTPYYGLLKEAVKRQASLIAKWQLVGFIHGVMNTDNMALSGETIDYGPCAFMDAYNP
                     DTVFSSIDTYGRYAYKNQPQIAVWNLSRFAESILPLIDENMEKSIKLAQDAVSEFFEL
                     YHNYWISGMRSKLGIFNGETEDEALVEDLLDLMHKNKEDYTNTFLKLTFGDTSIQRTQ
                     EFVEWHKRWQARLERQAQTKEESFELMKNSNPAVIPRNHRMEEALRAAEEEGDLSVME
                     RLMSILNKPFVHSADQKEYSDLPPSSFCDYKTFCGT"
     gene            10375..10448
                     /locus_tag="PKK75_03690"
     tRNA            10375..10448
                     /locus_tag="PKK75_03690"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10409..10411,aa:Pro,seq:cgg)
     gene            10516..11595
                     /locus_tag="PKK75_03695"
     CDS             10516..11595
                     /locus_tag="PKK75_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82729.1"
                     /translation="MELITVNKENRKYFLDFYRKQYINNELKRDALSGMVKNILYGKS
                     KLLSSVDIDPLMIVDNNEITMVSLLAYARRMPEYLQICFFESQIYNMDAFKLILNRAQ
                     EMAKEKKASKITGSLNIHVNYGLGFLASDFDKKQSFGAPYNPEFYNDYFKKSGFETIN
                     MVSYKKDMISSPPLISSDAREKLKDMYTIREVSFKNFKKEIEIYTKINNEAFKDHLFY
                     YTRDTEEDYELFKDLKYLMREENLIFAEKQGVPVGFMLWYPDFNEIIGKNEKPGIKTV
                     VKNKLFSKRIKTFKIVEIGVIPEERNKGAILALFDYCYKCTKGRYDTMESSWILSDNF
                     KSKGFGVKWADEEYKHYKAYVKEVI"
     gene            11592..12536
                     /locus_tag="PKK75_03700"
     CDS             11592..12536
                     /locus_tag="PKK75_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82730.1"
                     /translation="MIIYKADELKGMKTFDFNEEYIKLLSLLEKTNPCGQFYEAGEDS
                     FIISYELKLNLFNFKDFPALNTKAQITGFPISLSQRGYYGNLSKALEIIKKRKGLKII
                     LNADEEIIKNSRTLSTFVFDNSYKTFDDYLNALRSSYRRRINKALGYRHKLIINKIKN
                     SDFSGDHYNLYLSIMNRTDNPLETLPIEFFQSYETEIYEFAERETKKIIGFIQLKEVK
                     STLCFLFGGFNREDVKKYDLYYNMLLKIIEAGIEKKVNTIEFGQTAEESKLKIGCREV
                     DKFLYIHHSNYILNIIIQKLLPFVSYRPYKKIHHVFKE"
     gene            12541..13818
                     /locus_tag="PKK75_03705"
     CDS             12541..13818
                     /locus_tag="PKK75_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82731.1"
                     /translation="MIVFLEKVRVKTLFSIIDPVKVEPLELCYLKAVLNEMNIETYIV
                     DRLFAFSPPKDKTPDIIVVTGYNTAENEMIRRSEFYKSIYPNVKIIGGGVHVQENPEA
                     FRVSAIDYVFHSFSLNTFKLLIEKIINNDSIPLNCGLDTNIGGKWHLGEKESIYEIEN
                     IKPDRDFFYKAKSKLKYLDKRNVAIIKGSIGCPYNCSFCYCKLLNNKHFICGDYEKMF
                     EEMESIDADYYWIVDDVLFSTRNDALKFIEAAEKRNKDFSIIGYLRADFILREKDLLK
                     RLGDAGLREVITGFEATVNSELGEYEKTTDAGDYPLAISLLKENNIELTALFMVRPNY
                     GVKDFYNLIKFIKENKIDVYTVSIMTPMKKTKLYEEFEELLIDKRPERYDFLHLLIKP
                     KLPKFIFYTLFYGLHIRLLRSKRVRKFLFSKDR"
     gene            13823..14440
                     /locus_tag="PKK75_03710"
     CDS             13823..14440
                     /locus_tag="PKK75_03710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ82732.1"
                     /translation="MHNIWDYWAGKYDKLWVQKYSLKPTRDYIINALSKYINKDNLKV
                     LDLGCGTGELILELTKRFENIGITGIDFSEKMIEISKKRNPKALHLNMDVTNLSVLDD
                     EYDIIICTHSLPYYKKPKDVIRQLYNLLSDKGELLIGFASGNSTYDKFILSFVKLTTG
                     KAYYPSDNEFRIMTAPFFTVENLNIIKKKFFMPRIAVYTLKKVKS"
     gene            14437..15798
                     /locus_tag="PKK75_03715"
     CDS             14437..15798
                     /locus_tag="PKK75_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82733.1"
                     /translation="MKVLMVRPRPCKETIGLQHVMICEPLELEYLISNIPDDLKRETN
                     AEIVDMILEKTSFEEIVKIKKPDLLVFTGYISHVGTIKQMAKDAKKINPLIKTGVGGV
                     HAEVVPEDYRSEYIDFIYEKNGIDNFNLTLRGLINNKNVQEINEEINRGEKKTTFDYS
                     YPDRNAVKEYRKHYYYMFHNPCALIKTSFGCPYDCSFCFCKEITNGKYFTRQMGDIIE
                     ELASIEEEEIYIVDDDFLYDRNKLESFIEELGANNIRKKFLVYGRADFIARNKDIMIK
                     LKASGLQAVIVGIESIRTGDLEDYNKGTTIEINEKCIQILQELDIELYATLIIPLDFN
                     KEDFRQLREWLRKLNVRFVNLQPLTPLPGTEIFHMYKGTLLAKRHEYEVWDMAHVVLK
                     PKYMSIRDFYKEILKAYYKVIMRPKNFVYLIKKYGFKANLKMLAGSSFVSSQYIKKIL
                     RSH"
     gene            15798..17120
                     /locus_tag="PKK75_03720"
     CDS             15798..17120
                     /locus_tag="PKK75_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ82734.1"
                     /translation="MKKILLIQPSPYDKNHMPIKKYKLYFVGLAMPLLAAMMPDDWET
                     EIILEIIEDIPFDTDADLIGISTMGHGVIRSIDIAQKFRQMGKTVILGGYMASIMAEE
                     ALKHCDSLVVGDAELVWHELLKDYEEGNLKKVYEKNLESGFFSTPLPRFDLIINKRIG
                     NFLPVQAGRGCPNTCSFCSVSCLYKGHYIKKPLEEVIRDIKQVKELGFNRFLLLDDNI
                     FSDREYLDKLLMEIKKLNMHWMSQCDIRIGREEELLKKLADSGCTTLSFGLESISKES
                     LKGMNKAWANPLEYPYLIGNIHSHGIDVSTEMVVGGEGDTLSTIRDTKKFIEDNKITV
                     PRFYILTPIPGTKFFKDMTEAGRIIKDDIYSFDGTEAVHMPKNMTPGELTDAYWELYE
                     SLFTFRSILKRNIIRKEFLKKPDKYLFYAIVNLFYRYQIKQRITPNIF"
BASE COUNT         6212 a         2741 c         3490 g         4989 t
ORIGIN      
        1 atggtattac ttgtaatatg atttgtcctt catatgttag aacaccattg gttgacaaac
       61 aaattgctga ccaagctaaa acacatggaa taccagaaga caaagttata tctgatatta
      121 tgcttacaaa agcatttgtt aagaaattgt tggaacctaa ggatgttggc gagttcgcta
      181 aattcttatg ctcacctaca ggagattata taacaggtgc aatgcttact attgactgcg
      241 ggtggactgc atcttaaagt tattaaggag tgcatttgca ctcctcaaat aaaaaaataa
      301 aatggaggat attttatgtt tggaattatt ttaggtttag tattattaat gtttttagca
      361 tataggggat attcaatcct ttgggttgct ccggtatgtg cattggttgt tggtttaaca
      421 ggtggtcttg atttacttac aatgtataaa gatacttata tgactggtct ttcaggttat
      481 gtaaaatctt ggttcccagt atttatgtta ggtgcaatat tcggtcattt gatggactat
      541 acaggttcag caaaatcaat tgctcgttgg cttactaaaa aattaggacc aaaaagagca
      601 atactgggcg tagttgttgg ttgtggagtg ttgacgtatg gaggaattag tttattcgtt
      661 gttgtatttg ctatgtatcc tctggctttg gagttgttta aggaagctaa tattacaaga
      721 aaacttattc ctggtgcaat tgcacttggt tcatttacat ttactatgac tgctataccg
      781 ggaacaccgc aaattcaaaa tcttattcca atgaattact acgggactac acctactgca
      841 gcacctataa tgggtattgt tgcggctgtt cttatgtttg ggttaggtgt attttggctg
      901 cagtacagag agaagaaatt cacagcggca ggtgatgtgt atactgaacc aaagaataga
      961 gaaggtattg aagtggatta cagcgctctt ccaaacccat tcatatcatt tttaccactg
     1021 attactgtta tagtaacaat gaacgtcgtt gattgggata taatttatgc tttattaagc
     1081 ggcatcattc tttcaatggc aattaacttt ggtaaataca aaggatttac caagacaata
     1141 agcaacggtg cattaggttc tatgacagcc attttgaata cagctgcagc tgttggattt
     1201 ggtacagttg taaaagcagt gcctggattt gcttcattga cagcagtgct gctgaatgta
     1261 cctggaactc cgcttatatc agaagcaatt gcagttaact tacttgcagg agctacaggt
     1321 tctgcttcag gaggtatgag tatagcttta gaagcattag gtgaaaaata tatgcagata
     1381 gccctagaaa caggaatcag cccggaagca ttccacagag ttgcatcttt agcttcaggc
     1441 ggattagaca ctcttcctca caacggagca gttttaacgt tattagcggt aacaggattg
     1501 acacacaagg attcgtatat tgacgttatg atgacttcac ttgttcttcc tattatagca
     1561 tctataccgg ctattatatt aggcagtcta ggtatttact aaaattcttt agagaattat
     1621 catctaataa agatgtagca ctgctacatc tttattactg gtattgggac acacttcttt
     1681 aaagggaagg agacaacctc ttatataggg ataacctatg tggacagagg aatgtacacg
     1741 tacttagtat agtctttgaa gtccaaagaa gtgtacccaa ataaaatatt tttgtgtaag
     1801 aaaagcgtat caaaaggagg tataaatgaa attacaagac attaagaaaa tcggagttgc
     1861 cggcggcggc accatgggtt caggtattgc acaaatattt gctcagaacg gttatgaagt
     1921 tgcagtaact gatattgcag aaaagttcct ggaaaatacc aaaagaatta tcctgctgaa
     1981 tcagaaaact ttaattaaag aggggttatt aactcaggaa caagctgaag aatcattgaa
     2041 attaattaca tattcaacag acaatggtgt ctttgttgat gcggatttaa tagttgaagc
     2101 aataattgaa aaaatggata ttaaacaaga ctactggaaa gaagttgaag ggatagcacc
     2161 taatagttgt atttttgcta ctaacacttc aggattaagt attaatggaa tatgttcaaa
     2221 attgaataat aaaaaaagat ttataggaat gcactggtgg aatcctcccc atattattcc
     2281 tttgattgaa cttattaaaa acgatgaaac atctgatgag accgtcagga ttttaaaaga
     2341 attagttgat aaaataggta aagaatccgt tgtagtatta aaagacgtaa atggattcat
     2401 aggaaacaga attcaattcg ctgtttatag agaagttcta aaaattatcg aagatggggt
     2461 tgcgactatt gaggatgttg acaaagctat gaaattcgga ccgggattta ggtatcctgt
     2521 gctcggacct tttgagacat ctgatttggg tggactggat acattctact atatttcatc
     2581 ttacttattt aatgatttaa gtgatgtgaa aaagccgact aagcttcagc aggaaaaaat
     2641 ggacaatggc catcttggcg tgaaaacagg aaagggctgg tatgattact ctgacggtaa
     2701 ggatgtagaa actatgacaa gaagggatga aaactttttt agaatgtaca aatcatacat
     2761 aaccggcaag tataaaaaat aatcagtaat aaagcatgca taaaaacctg ccaacagata
     2821 aaggcaggtt ttttcctgct acacgttaaa tctgaacaat actatatctc cgtcttccat
     2881 tacatattcc ttgccttcaa ggcgcattaa tcctttttcc tttgcagcct gcattgtgcc
     2941 caggttaaaa acatcatcgt actttgtaac ttctgccctt atgaagcctc tttcaatatc
     3001 cgagtgaatt ttgccggcag cctggggggc ttttgtgcct ttgacgattg tccatgcctt
     3061 tgattccatg gggcctgttg tgaagaaact aatgagtccc aaaagccgat agcttattct
     3121 aataagctgg tcaaggcctg attctgcaag tcccatttcc attagaaatt cctttttttc
     3181 atcatcttcc aacacggata tttcttcttc gattttgccg cagataacga ttacttcaga
     3241 gttgtctttc gaagcgtatt tttttacaat atctacaaat ctattgtccg aggggttgga
     3301 aagatagctt tcatcaacat ttgccacata caaaaccggt tttgaagtta tcaggttaaa
     3361 tgtttttaag attttaagct cttcgtccgt atatgtcaag taacgtgcct gtttttcctt
     3421 ttccagtcct tcagtaatct ttttaaccag ttcaaattca ggctgtaggg atttatcatt
     3481 ttttaccgtc ttggaaagct tgtcaagccg ttttgacata acttccaggt cagctaaaat
     3541 caattccagt tctattattt caatatctct ttcagggtct acagtagttt caacatggat
     3601 tacattttcg tcttcaaaac atctgaccac atggacaaca gcttcaacct ctcttatatg
     3661 tgaaagaaac ttgtttccta atccttctcc cttgcttgca cctctgacaa gacctgcaat
     3721 atcaaaaaat tctataactg ccgggactgt ccttgcagaa ttgctcattt ttgttaagaa
     3781 atccaatcta ttatccggaa cctccacaac ccctatatta ggctctatgg tgcaaaaggg
     3841 gtaattggca gaatccgcac cggctgaagt aattgcattg aataatgtgc tcttgcccac
     3901 gttaggaagt cctactatac ctaatttcat attttaacca tccttatctt ttacttagta
     3961 aagaatcacg cactgacttg ttgaattatg tttattatat aatatatgat tttaaacttc
     4021 aataattata gcaggagaaa actgaaaaaa tacatctttt taggatttga aagacatata
     4081 ttccattgcc ttgaatatta ataaaatata taagacaaat tataggggga agaaaatata
     4141 tgaatgataa acagatgcag tccaattata ttaaaatagt cggaataata ttcagcgaat
     4201 tggaattcag tcacgaggtt tttggagaaa aattttttga attctttata gaagtaccaa
     4261 gattgagcga aaccaaggac acactgcctg ttataatttc cgaaaggttg attaatgata
     4321 taaatatgga tattggaaat tacatagtta ttgaaggaca gttcagatcc tataacagat
     4381 atgaggatgc ccaaaacaag ctcttgttaa gagtatttgt aagagatatt tatgtaccgg
     4441 attataccga acttgaagaa atgaaaagac atccaaatga agttttttta aacggatatc
     4501 tctgcaaaga aacaaaattt cgaacaactc cattcggcag agaaataact gatatgctta
     4561 ttgcagtaaa ccgttcttac aacaagtccg attacatacc ttgcattgca tgggggagga
     4621 atgcaaggta ctgcgaaaaa ctggaggtcg gggaccatat aaaaatttgg ggaagaatac
     4681 aaagcaggag gtatcaaaag aaaaacgaaa acgaatccta tgatacaaag acagcttatg
     4741 aggtgtcagt tacaaagctg gagcatataa agacagaaaa caacaggaaa aaagaagatg
     4801 aggatatgga attataatat ataaacctaa gaaaccgaaa ggtttctttt taaatataaa
     4861 aacaatttat taatttgaca gattatgata caataacaag aaaaaaaaga aaattaaagg
     4921 aaattataat gatattagta ttggcggaaa aaccatccgt gggcaaggat ttgggaaggg
     4981 ttttaaattg cagcaagtca ggaaatggat ttgtggaagg cagcatatat attgtcacct
     5041 gggctttggg acacctggtt acattagccg accccgaaaa atacaacccg aaatatgaac
     5101 gctgggaatt agaggatttg cccattatcc cgaaaaggat ggaaacagag gtaattaaac
     5161 aaacaaggaa gcagtataac ctggttaagg agcttatgca tcgaaatgat gtaaaagaaa
     5221 ttgtaatagc aactgatgca ggaagagaag gggaattggt agcaagatgg attatagaaa
     5281 aagccgccgt taataaacct ttgaaaagac tgtggatttc atccgttaca gacaaagcca
     5341 taaaagaggg atttaacaat cttaaagacg gcaaagccta tgaaactctt tacgcctctg
     5401 cgctggcacg gtctgaagct gactggattg tcggcataaa tgcgacaaga gcattgacaa
     5461 ccaaatacaa tgcccagtta tcctgcggaa gggttcagac tccaacagtg gcaatgattg
     5521 cagccttaga ggatgaaata agggacttca ggccggtatc ctattatgga atagaagctg
     5581 cagccggaag cattaaattc acatggaatg aaaataacga tactaaaata tttgacgaaa
     5641 gtaaatgcga cgctataata gacagcataa agggcagtaa attaaagctc accgacatta
     5701 caaggaagaa agcactttct tattcacctc ttttgtatga cttaactgag cttcaaagag
     5761 atgcaaacaa tattttcgga tttaccgcaa aggagacatt gtccgcaatg cagactctct
     5821 atgagcgcca caaggtattg acctatccca gaacggattc caggtattta actgatgata
     5881 ttgttgaaac attaaaagac agggtaaaag ctgcaagcgt gggaccttac tcaagaatag
     5941 gatttcaaat aagccaaaat ccaattaaag ccagcaaaaa ctttgtaaat aacagcaaag
     6001 tttcggacca tcatgccatt attccaactg agcagactgt aatcttaagc gagctgacag
     6061 acagggaaag gaaaatatat gatttggttg ttaaaagatt tttcgccgtt ttatctaagc
     6121 cttttgaata tgaaaaaata tgtgtaacag gaataattaa taatgaagaa tttatatcca
     6181 aaggcagaat cacactgtcc atgggatgga aggaagtata cggaaactat gaggatgagg
     6241 aaatccttga tgagtatcct gtagaagagc tgaataaatt aaaaacaggc agtgagttat
     6301 ctgtttctaa agtttataaa acaagcgggg aaaccaagcc tccttccaga tttacagaag
     6361 cagccctcct gtctgccatg gaaaatccgg taaaattcat gaaaaacgca gacaaggacc
     6421 ttaaaagaac aatcggagaa accgggggtc ttggaacagt tgcaacaaga gcagatataa
     6481 ttgacaaact ctttaacagc tttgttatag aaaaaaagga taaatacatt tataccactt
     6541 ccaaggggag acagctttta gaccttgctc ccgcagactt gaagtctcct gttttgactg
     6601 cccaatggga gcagaagctg aatgaaattg ccaggggaaa cctggacaag aataaatttt
     6661 taaatgatat gattaattac actaaaacaa tcataaagga aatcaaaagc agcaacaaaa
     6721 gctacattca tgacaacgaa acaagagaaa aatgtcctga ttgccaggaa taccttttgg
     6781 aagtcaaagg caaaaaagga actatgctgg tttgcaaaaa cagagagtgc ggttacagga
     6841 gaggaatttc acaaattacc aatgcaagat gccctgactg ccataaaaag ctggagctta
     6901 aaggagaagg ggagggcaga ctctttgtat gcaaatgcgg ttacagagaa aaactatcga
     6961 catttaacaa aagaaaggct aatgagaagg gatcaattac caacaaggaa gcatccggat
     7021 acatgtacaa acaaaataaa aaacaagatg aaagcattaa tactgctttg gcagatgctt
     7081 tgaaaaaact taatttttaa tatggggaga caaaatgcat tatgtaatta ttggtttatt
     7141 tgcagtttat attatttatt tgtttataag aagaaacagc ttaaaaattc aggttgaaca
     7201 gtttaacaaa ctcttgtatg ctgataaaaa acctgaccaa tatattcagg aaatagataa
     7261 tcttcttgca aaacgccagt ctaaaacaga aattgatata aattatatac aaaagtcaac
     7321 aggtcttttg tatgccggca ggtttgacga ggcagcaggc atattaactg aaaaagtgaa
     7381 taaaatacct aatttctggc aggtagtata ttaccacaac ctgcttttaa gcttatattt
     7441 cggcggcagc acagaaaaag ccaatggtat tttaaacgag gttaaggatg ttatggattc
     7501 atattataaa aaggattaca acaaagttac aattgaactg attaatgccg tttctgacta
     7561 ttacaacgac agaatatcag aatccaggga tttttttaat aagctgcctg atattacatc
     7621 aaatgaatac aggaaagcaa tgggctatta ttataccggc aaaatattgg aaaatgaagg
     7681 gaaaaaggaa gaaagcaatg aattgcttga aaaggcgaag acctgtgcac aagggaattt
     7741 cattgaatat ttataggtgt tgaatatgga tattgtacaa atggacaata agattacaat
     7801 acatgacgtg aaggatttta acacaaggca tatttttgag tgcggccagt gcttcagatg
     7861 gaataaggaa gaggacgggt catatacggg agttgttaaa aacaatgtta taaatctgcg
     7921 gcaggaaggc agcactgttg aatttaacaa tatttccgat tatgatatca taagaagcta
     7981 ctttgacttt gacacggatt acgcagtgat taaaaaagca ttgaacacag atgaaattat
     8041 gaaaaacgcc attaattacg gagagggcat aagaatactg gctcaagatg agtgggaaac
     8101 aatgatttct tttatgattt cagcaaacaa cagaattcct atgattaaaa aggttataga
     8161 aaatttatgt acttgctttg gcgaatacat atgcaactac aggggcaggg attattacag
     8221 tttcccgtcg gcggaaagct tatctcaagc tcctgttgaa gaaatttttc agtgcaaggc
     8281 aggtttcaga tctcccagga taaaggaagc tgcagcaaga ttcttacttg aaagggacca
     8341 aatatacaac atcagaaaca tggactatga agatggactg gcttatttaa aaacttataa
     8401 gggaataggg gacaaggttg ccaactgtat tttattgttt tcaaccaggc attttgatac
     8461 attccccgtt gatgtctggg taagaagaat aatgcagaca ctttatttaa gcaaggatac
     8521 taaggatgta gatataagaa aatttgccga ggaaaaattc gggaagtatt caggctttgc
     8581 ccagcaatat ctttttttct acgcaagaga aaacttaata gggaaataaa gatgccctaa
     8641 ccccattttt tctgagtttt ttttatataa aaataataaa ttctataatt ttctaattct
     8701 tctaataaaa ctacaggtta atcatgtctt tcatgtaaat aatatataaa aacaatataa
     8761 tggagaaata atgacaaata caaaaactaa aacaggctgg aatttagaaa acagctacgc
     8821 aatgctgccg gatttttttt attccaaatt aaatttaagc cgggtaagtt cacctaaatt
     8881 agtggtttta aacaatgaat tagccaaata tttaggcttt gatataaatg aacttatcag
     8941 cgctgaagga ttatcttatc tttcaggaaa tgatgttcct gaagggtcaa taccaatagc
     9001 gcaagcctac gccggtcatc agttcggtta ttttacaatg cttggcgacg gcagggcaat
     9061 gctgttggga gaacaaaaaa tacctgaagg aaaaaaatat gacattcagt taaaaggctc
     9121 gggcaagact ccatattcac ggggaggaga cggaagagca gcacttgggc caatgcttcg
     9181 tgaatacatt gtaagtgaag caatgcacgc actaaacatc cccacaagca gaagcttggc
     9241 ggttgtaact acaggcaagc ctgtaataag agaaaagctc cataaaggct caattttaac
     9301 cagagtggca ggcagtcata ttagggttgg aacatttcaa tacgccgctt atttcggaac
     9361 ggatgaaaat ataaggcaat tagctgatta tacaattgac agacacttca aggaaattga
     9421 aaagaatgac acaccttatt atggactttt gaaagaggcg gttaaaagac aggcatcctt
     9481 gattgcaaaa tggcagcttg ttgggtttat tcacggtgtg atgaacacag acaatatggc
     9541 tttaagcgga gaaacgatag attatggtcc ctgtgcattt atggatgcct ataatcctga
     9601 tactgttttc tcttctatag acacctacgg acgttacgcc tataaaaatc agccgcaaat
     9661 tgctgtatgg aatttatcga gatttgctga aagcatttta cccctgattg acgaaaatat
     9721 ggagaagtca attaagcttg ctcaggatgc agtttcagaa ttttttgaac tataccataa
     9781 ttactggatt tccggtatgc ggtcaaaatt aggaattttc aatggggaaa ctgaagatga
     9841 agctcttgtt gaagacctgc ttgatttaat gcataagaat aaggaggact atacaaatac
     9901 ctttttaaaa ttaaccttcg gcgatacttc catccaaaga actcaagaat ttgtagaatg
     9961 gcacaaaagg tggcaggccc gtcttgagag gcaggctcaa acaaaggagg aatcatttga
    10021 actaatgaaa aattccaacc ccgcagtcat acccagaaac cacaggatgg aagaagcttt
    10081 aagggcagcc gaagaggaag gggacttaag tgttatggag aggttgatga gcatattaaa
    10141 taaacctttt gttcattcag ctgatcaaaa agaatattca gaccttccac cctcatcgtt
    10201 ttgcgattac aaaacctttt gcggaacata agaagaggga cacaagactt tctagcgtga
    10261 tagtctctgt aggtaagtgg aatgtccacc tcagacaaca cccgaagata gtgaacgcaa
    10321 gggggaaaat ttaattgaca aaaatcttca ttgagcttat aatgttaatg ttgtcggggt
    10381 gtagcgaagc ttggtatcgc gcatggttcg ggaccatgag gccgaaggtt caaatccttt
    10441 cactccgatt tttttatttt tttgtaatag tttttagccc gtgctataat gtactataaa
    10501 atttatggag cagccatgga attaataaca gtaaacaagg aaaacagaaa atacttccta
    10561 gacttttaca gaaaacaata tataaataat gaattaaaaa gagatgccct gtcaggcatg
    10621 gtcaaaaata ttttatacgg aaaatctaag cttttaagct cggtagatat tgaccctctt
    10681 atgatagttg ataataatga aataacaatg gtatccttgc ttgcctatgc ccgcaggatg
    10741 cctgaatatc tgcagatatg cttcttcgaa tcacaaatat acaatatgga tgcttttaag
    10801 cttattttaa acagggctca ggaaatggca aaagaaaaga aagcttctaa aataacagga
    10861 tcacttaaca tacatgttaa ttacggtctt ggatttttag caagcgattt tgataaaaag
    10921 cagagtttcg gggcacctta caatcctgaa ttttataatg attattttaa aaaaagcggt
    10981 tttgaaacaa ttaatatggt gagttataaa aaggatatga tctcatcacc tccccttata
    11041 agctctgatg caagagaaaa gcttaaagat atgtacacaa taagagaagt aagtttcaag
    11101 aattttaaga aggaaataga aatatatact aaaataaaca atgaagcatt caaggaccac
    11161 cttttttact acacaagaga tacagaagaa gattacgagc tcttcaaaga cttgaaatat
    11221 ttaatgaggg aagaaaatct tatttttgct gaaaagcagg gagttcctgt aggttttatg
    11281 ctttggtacc ctgattttaa cgaaataata gggaaaaatg aaaaacccgg cattaagaca
    11341 gtggtaaaaa ataaattatt ttccaaaaga ataaaaacat ttaaaatagt tgaaataggt
    11401 gtcatacctg aggaaagaaa caaaggagcc atacttgcat tgtttgatta ctgctataaa
    11461 tgcacaaagg gcaggtatga taccatggag tcaagctgga tactttcaga caattttaag
    11521 tcaaaaggct ttggcgttaa gtgggctgat gaagaatata aacattataa agcatatgtt
    11581 aaggaagtta tatgataata tataaggctg atgaacttaa aggaatgaaa acatttgatt
    11641 ttaatgaaga atatattaaa cttctgtcac tgcttgagaa aactaatccc tgcggccagt
    11701 tttatgaagc gggagaggat agttttataa tttcctacga gcttaagctt aacctgttta
    11761 attttaagga ttttcctgct ttaaatacaa aagctcaaat tactggcttc cccatatcct
    11821 tgtcacaaag gggatactat ggaaatttaa gcaaggctct tgaaatcata aaaaaaagaa
    11881 agggacttaa aataatatta aatgcagatg aagaaattat aaaaaactca aggaccttgt
    11941 ccacctttgt ttttgataac agctataaga cttttgatga ttatttaaat gccctcagaa
    12001 gctcttacag aaggcgtata aataaggctc tgggatacag gcacaagctg ataatcaata
    12061 aaataaaaaa cagtgatttc agtggagacc attacaactt gtatttaagc ataatgaaca
    12121 gaactgacaa tcccctggaa accctgccta ttgagttttt tcaatcatat gaaacggaaa
    12181 tttacgaatt tgcagaaaga gaaacaaaaa aaattatcgg attcatacag ttaaaagaag
    12241 ttaaaagcac actttgcttt ttatttgggg gatttaacag ggaagatgtt aaaaaatatg
    12301 atttatacta caatatgctt ttaaagatta ttgaagcagg tattgagaaa aaggttaata
    12361 ctatagaatt cggccagacg gcagaagaaa gcaaattaaa aatcggctgc agggaagtag
    12421 ataaattcct ttatattcat cacagcaatt atattttaaa tattataata caaaagctgc
    12481 ttccttttgt gtcatacagg ccatacaaga aaatccacca tgtttttaag gaatgataat
    12541 atgatagttt ttttggaaaa ggtaagggtc aaaacattat tttcaattat cgaccctgta
    12601 aaggttgagc ctttggaatt atgctattta aaagctgtat taaacgaaat gaatattgag
    12661 acctatattg tggaccggct ttttgctttt agtcctccaa aagacaaaac acctgatata
    12721 attgtggtca caggctacaa tacggcggaa aatgaaatga taagaaggtc cgaattttat
    12781 aaaagtattt atcccaatgt aaaaataata gggggcggag ttcatgtgca agaaaatccg
    12841 gaagcattca gagtatctgc aatagactat gtttttcact catttagctt aaatacattt
    12901 aaactgctga ttgaaaaaat aataaataat gacagtatcc ctttaaattg cggacttgat
    12961 acaaatattg ggggaaagtg gcatcttgga gaaaaagaga gtatttacga aattgagaat
    13021 attaagcctg acagggattt tttttacaag gcaaaatcaa aacttaaata tcttgataaa
    13081 agaaatgttg caattataaa gggaagcata ggatgcccct acaattgctc attttgctac
    13141 tgcaagcttc ttaacaacaa gcattttata tgcggggact atgagaaaat gtttgaggaa
    13201 atggaaagca tagatgcgga ttattactgg atagttgatg atgtgttgtt ttcaacaaga
    13261 aatgacgcct tgaaattcat tgaagctgca gaaaaaagaa ataaagactt ttccattatt
    13321 ggatatttaa gggcagattt catactaaga gagaaggacc tgttaaaaag gcttggagat
    13381 gcaggcctta gggaggttat cacaggtttt gaggcaactg ttaacagtga gcttggcgag
    13441 tatgaaaaaa ctaccgatgc gggagactat cccttggcaa tatccttatt gaaagaaaac
    13501 aatattgaat taacagccct ctttatggta aggccaaact atggagtcaa ggatttttat
    13561 aatttgataa agtttattaa ggaaaacaag atagatgttt atactgtatc aatcatgact
    13621 cccatgaaga aaacaaagct atatgaagaa tttgaagaac ttttgattga taaaagaccg
    13681 gagaggtacg atttcttgca tcttctgata aagcctaaac tgcccaagtt catattttat
    13741 actttgttct atggacttca cataaggctt ttaagatcga aaagagttag aaaatttcta
    13801 tttagtaaag acaggtgagc atatgcacaa tatatgggat tattgggcag gtaaatatga
    13861 caagctgtgg gtgcaaaagt attccctgaa acccaccaga gattatataa taaacgccct
    13921 ttcaaaatat ataaataaag ataatttaaa ggttctggat ttaggatgcg gtaccggaga
    13981 attaatactt gaattgacaa aaagatttga aaacattgga attaccggga ttgatttttc
    14041 agaaaaaatg attgaaatat caaagaagag aaatccaaaa gctttgcatt taaatatgga
    14101 tgttacaaat cttagtgtcc tggatgatga gtatgacata ataatctgta cccattcact
    14161 gccttattac aagaagccga aggatgttat cagacaattg tataatttat taagtgataa
    14221 gggggaatta ctgatagggt ttgccagcgg taacagcaca tatgacaaat ttatattaag
    14281 cttcgtgaag ttaaccacag gcaaagcata ctatccttcc gataatgaat tcagaattat
    14341 gacagctccc tttttcacag tggaaaattt aaatataatc aaaaaaaagt tttttatgcc
    14401 aagaatagcg gtttacactc tcaagaaggt gaaatcatga aagtattgat ggtaaggcca
    14461 agaccctgca aagaaacaat aggcctgcag catgtaatga tttgtgagcc tttggagctt
    14521 gaatatttga tttctaatat tccggatgat ttaaagaggg aaacaaatgc tgaaattgtg
    14581 gatatgatac ttgaaaaaac aagctttgaa gagattgtaa aaattaaaaa gcctgacctc
    14641 ctagtgttta caggctatat ttcccatgtg gggacaataa aacaaatggc aaaggatgca
    14701 aaaaaaatta atcctttgat taagacagga gtcgggggag ttcatgcaga ggttgtgcct
    14761 gaggattaca ggtctgaata tattgatttt atatacgaga aaaatggaat tgacaatttt
    14821 aatttaacat tgcggggttt aataaataat aaaaatgtgc aggaaataaa tgaagaaata
    14881 aatagaggtg aaaagaaaac caccttcgat tacagctatc ctgacagaaa tgctgtaaaa
    14941 gaatacagga agcattatta ctacatgttt cataaccctt gcgcccttat aaaaacttcc
    15001 tttggatgcc catatgactg ttccttctgc ttttgcaagg aaattacaaa cggcaaatat
    15061 tttacccgcc agatgggtga cattatagaa gaattagctt caattgaaga agaggaaata
    15121 tatattgtag atgacgattt tttatatgat agaaataagc ttgaaagctt cattgaagag
    15181 cttggggcaa ataacataag aaagaaattc cttgtttacg gaagagctga ttttattgca
    15241 cgaaacaaag atataatgat aaaacttaaa gcttcgggtc tgcaggctgt aatagtggga
    15301 atagaatcca taagaacagg tgacttagag gattacaaca aggggacaac catagaaata
    15361 aacgaaaaat gcattcaaat attacaggaa ttagatattg aactgtatgc gacattgatt
    15421 attccacttg attttaataa ggaagatttc aggcagctaa gagaatggct gagaaaatta
    15481 aatgtaagat ttgtcaacct ccagcctctt acaccccttc cgggaactga aatattccac
    15541 atgtacaagg gtacgctttt ggccaaaaga catgaatatg aagtgtggga catggcgcac
    15601 gttgttttaa agccaaagta tatgagcatc agagattttt ataaagaaat attgaaagca
    15661 tattataagg taatcatgag accaaagaat tttgtatacc tgataaagaa atacggtttt
    15721 aaggcaaatt taaaaatgct ggcgggttca agctttgtaa gctcccagta tattaaaaag
    15781 attttaagga gtcattgatg aagaaaatat tattgataca gccaagcccc tatgacaaaa
    15841 atcacatgcc cattaagaag tataaattat attttgtggg acttgccatg cccttactgg
    15901 cagcaatgat gcctgatgac tgggaaacag aaataatcct tgaaataatt gaggatattc
    15961 cttttgatac agatgctgac cttataggaa taagcaccat ggggcacgga gttatccgct
    16021 caattgatat tgcacagaaa tttcgccaaa tgggaaagac tgtaatactg ggaggctaca
    16081 tggcaagcat aatggctgag gaagcattga agcactgtga cagccttgtt gtcggtgatg
    16141 cggagcttgt ttggcatgag cttttaaaag attatgaaga aggaaatcta aaaaaagtat
    16201 atgaaaaaaa tctggagagc ggtttctttt caacaccact tccaaggttc gacctaataa
    16261 taaacaaaag aataggaaat tttcttcccg tccaggcggg aagggggtgt ccaaacacat
    16321 gcagcttttg ttccgtctca tgcttgtaca agggacatta tataaaaaaa cctcttgaag
    16381 aagttataag ggatattaaa caggtaaaag aattaggttt taacagattt cttttattgg
    16441 atgacaatat tttttctgac agagaatatt tggacaaact gttaatggaa attaaaaaat
    16501 taaatatgca ctggatgagc cagtgtgaca ttcgcatagg cagggaagaa gaacttttga
    16561 aaaaactggc ggacagcggc tgtacaacct taagtttcgg ccttgaatcc atttcaaagg
    16621 aaagtttaaa gggaatgaac aaagcctggg caaatccctt ggaatatcct tatcttatag
    16681 gaaacatcca ctcccacggc atagatgtat caactgaaat ggttgttgga ggagaaggtg
    16741 ataccctttc tacaataagg gatacaaaaa aatttattga agataataaa ataacggtac
    16801 cccggtttta tatactcacc cccatacctg gaaccaagtt tttcaaagat atgactgaag
    16861 cgggaagaat tatcaaggat gacatttatt cctttgacgg gacggaagct gtccacatgc
    16921 ctaagaatat gactcccggt gaattaacag atgcatattg ggaattgtat gaatccttgt
    16981 ttaccttcag gtcaatttta aaaaggaata taataagaaa agaattctta aaaaaacctg
    17041 acaagtattt attttatgct atagttaatt tgttttacag atatcagata aaacaaagaa
    17101 ttacaccgaa tatattttaa gaaatgccgg aagaaaaata ataaaaatat acggtcacgg
    17161 tttcggacac gaataattct atagcttatt tcagctataa atcctgcagc ttgcaaactc
    17221 gctttgctca aacagtgcaa gctgcttaac ggatttcttg cctgtatttg ctaagaatta
    17281 ttatcgcgtt atgcaaatgt tcgctaaata tttttattat ttttcttaag tgctgcgaaa
    17341 taacaaggat aaaagttagg agccacaatg aaaatactat tggtaagacc gccgagaatt
    17401 aaacaggcaa taacactgag cgattttatg tt
//
LOCUS       DAOPUT010000024        17138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3390_length_17138_cov_22.268161, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000024 DAOPUT010000000
VERSION     DAOPUT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 17138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17138
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3390_length_17138_cov_22.268161"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKK75_03725"
     CDS             complement(<1..443)
                     /locus_tag="PKK75_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010631757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNZ82735.1"
                     /translation="MNKVDYKKDFKDLYLPKSSPCIIEVPEIRFAIIDGRGDPNGEEF
                     SHATAALYSFSYAVRMSYKSKVIPGGFYDYTVFPLEGVWDLVDKSKSIADKSNFAYSI
                     MIRQPDFLTNELFERFLTETKKKKPNAYLDKMKYDTITEGLCCQML"
     gene            complement(548..1354)
                     /locus_tag="PKK75_03730"
     CDS             complement(548..1354)
                     /locus_tag="PKK75_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ82736.1"
                     /translation="MKLTVLIDNNTYIDKYYFGEPALSFYIEDDESKILFDTGYSGMF
                     IKNAHDLNIDLDSVTAIAISHGHNDHTGGLFYLAAENKIAGKNIIAHPNAFDRKYCDG
                     EEIGSILSLEELKKLCKVTLTKEPVRISPNITFLGEIPPYNDFETRKAIGTVSAEDKE
                     QPDYIIEDTALVYNSGGGLFIITGCSHNGICSIIEHAKKICSEERVIGVIGGFHLFDV
                     SDQLKKTIEYFLRNKISRLYPCHCVSFAAKAEIHKFIPVEEVGVGLTIEI"
     gene            complement(1417..2103)
                     /locus_tag="PKK75_03735"
     CDS             complement(1417..2103)
                     /locus_tag="PKK75_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82737.1"
                     /translation="MEIINNIISKFNKIINKINNPVEVDPRRFNDPVAMITEWSPLPN
                     ARGANFCTGRLVQTDELVIKFRPTPGVIIFFSAFVLVSVAFPLIFMRPPFRGSKFLVI
                     IPILFMAVGLYGLFKFTRPVVFDKRSGLFYKGRRKTDLNDSKKQNKRCILLDDIYAIQ
                     LLSSFVSSQDSDGMDHSYYSYELNLVMNDGDRINVLSHGNLLNIRTDAQTLSRFLNVK
                     LWDTIRDGSY"
     gene            complement(2158..2325)
                     /locus_tag="PKK75_03740"
     CDS             complement(2158..2325)
                     /locus_tag="PKK75_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82738.1"
                     /translation="MKLIQVHKEDYITLKGYLVNVKFPDYDWKSSLARNDTGNGACEI
                     MYVESVTRADT"
     gene            complement(2349..2771)
                     /locus_tag="PKK75_03745"
     CDS             complement(2349..2771)
                     /locus_tag="PKK75_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82739.1"
                     /translation="MKKELVIFLIEILIIAVITFIILPGLNDKEVMMIGEPVQVDIKT
                     GFNNEINHDGNNKTIKMEKLAEYKISAVVKSKRYYFFDSASEVSPIDLLLVWGELDTK
                     EMDDSINYSQSGRCIFTGMAAKVVLTVITYLSIQPMYI"
     gene            complement(2969..3463)
                     /gene="ybaK"
                     /locus_tag="PKK75_03750"
     CDS             complement(2969..3463)
                     /gene="ybaK"
                     /locus_tag="PKK75_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNZ82740.1"
                     /translation="MAKNTEKQGEQKTNVMRLLDKEKIPYRHHCYADTDAVSGLEVAA
                     VLGQDIDRVFKTLVAVGRSGEHYVFVVPVSRELDLRKAAKTVNEKSVEMIKAKELLPL
                     TGYIHGGCSPIGMKKRFTTVVDESASGKDTIFVSAGKIGYQIEIIPDDLRRIIPFLFA
                     DITE"
     gene            3798..4844
                     /locus_tag="PKK75_03755"
     CDS             3798..4844
                     /locus_tag="PKK75_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82741.1"
                     /translation="MKDNKIKIAILGFGNVGKAFAKLLIEKEEEIFDKFSLKVVVSAI
                     STKSKGCLINENGIDLEGALGDLEKFNHFDEKRCDYNEMSSMDVAENADYDILFELTP
                     LKIFSGQPAIDHISSALKRKKHAVSANKGPIAWAYKPLKELSKKNNVLFYYETTVMDG
                     TPVFNLVDETLKFCKILKIEGILNTTTNFVLEEIAKGKSYDEAIAEGKRRGFVEEDPA
                     MDIEGWDAAAKTAALLNVLMNADITPYDVDRKGIEDVTLEQIKEAESRGNVIKLLCYG
                     KIEDGKVIARVQPEEISKNSLLSNINGTTSIVSITTDLMATVSIIEHEPEIEQTAYGV
                     FSDLIRVLSNMKCL"
     gene            4911..7466
                     /locus_tag="PKK75_03760"
     CDS             4911..7466
                     /locus_tag="PKK75_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ82742.1"
                     /translation="MKKFTFFTIIVLLLALIMVSINIEEIINIFIEKQEQHEEIINKD
                     SIKNYFPIRQNIKYVYEASSDDLLYEVTPDYTSEGMIQLRITGNEGVTVSVIKVNDKK
                     ATGRIMMQENLLRENLLNSAKLHNQDDMEDVLLMEPLATGTEWTVSGNHQRKITNTDS
                     IVETDIGTYDAIEVTTESVNYKKVDYYTKGIGLIKSVITSEDKTTTIVITEIIENSPF
                     TENIEFYYPDFNKMKILYKVQNVEFKTNDLTETVIAEAYKKALPKENSVLTKETGINR
                     IFWDAGGTLCIDLNKAFIDNAKKKALHEGMVLQSIANTFGRFFNTEQVYFTVDDENYA
                     SDHIDLSQGTFAVNFIGYPLFYDIVIYGGTPSGITAAVSAAREGMDVALIEQGSHIGG
                     MVTGGLGFTDRGNTKIIGGITREVFEKIGRHYGKDAGWDFEPHVAENVLLEIIEKEEI
                     DLYYNKKIKEDNGIEKDGKKIISIETDDGDLFFAQVFIDSSYEGDLMAMSNVSYTTGR
                     EDSKKYNESFAGLLPPMGTNNFYYNLNAHDEDGSLYSGILNEYPGAINRGDKKIQAYN
                     YRLCVTNQPENRIPFYKPNNYNEENYKLLAAWLNKIKEIENRNLAFSDVVYLGRLPNN
                     KYDVNNYGPFSTDLIGGSWEYPDADYERREEIKNEHKEYIQGLLYFISTDSSVPAELR
                     EDVKQWGYAADEFEDNHYWPYQIYVREGRRMVGDYVLTESDIRKEKTKYDSVGMGSYS
                     IDTHNVQRFLTKDGFVLNEGELQYPVTPYEIPYRVMIPKAAEADNLIVSVCISASHIA
                     YSSVRMEPQYMIMGEAAGLAAKISIDDNTNVQNINYNKLKAKLTENVAVLRFE"
     gene            complement(7633..8508)
                     /locus_tag="PKK75_03765"
     CDS             complement(7633..8508)
                     /locus_tag="PKK75_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNZ82743.1"
                     /translation="MTNSFKNVKRVLLIILIANLGVALLKIVIGSIVKSSSMTADGFH
                     SLSDGSSNIVGLIGIYFASKPVDEDHPYGHGKFEMLAGLFIAGMLFFIGFKVIFNTAE
                     RIMNPIIPKVSIESLIVLVVTLCVNIFISKYEYAKGRKFNSQILISDSMHTRSDIYVS
                     IGVLITLTCIRFGLPAIIDSIASFAVAGFILHAAYEIFRDNSGILLDKAAIDNSKIET
                     IVLSYEQVKNAHNIRSRGYGNALFIDMHIMTEPEMSVEKSHELIHNIEETIRMEINGN
                     VQVTAHLEPYKQLKY"
     regulatory      complement(8563..8646)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
     gene            complement(8820..9320)
                     /locus_tag="PKK75_03770"
     CDS             complement(8820..9320)
                     /locus_tag="PKK75_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82744.1"
                     /translation="MSIRNITQGGLLICLSVLLQLLPAAFGEIFIIATILSAVPIYIL
                     SRSNPKAGFTAYIITGVLIYTFNSHEGMFFLFTNGLVGFSIGAFSYITKSKIIISILS
                     GVILTFSLAIVNFLIGIPVLGVNFPGSILIQIPVLMAFSIVYCFMYSLFADFTYNYLN
                     RRYPFR"
     gene            9431..9739
                     /locus_tag="PKK75_03775"
     CDS             9431..9739
                     /locus_tag="PKK75_03775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ82745.1"
                     /translation="MLTELPFADKSFDVSITSFVAHGLSKNERQVMYDEMIRITKHLV
                     IIYDYNEHRGLITDLAEWLEGGDYFNFIKTIKNELNDKFEDLTIINANIRSALYIAKL
                     "
     gene            complement(9882..10454)
                     /locus_tag="PKK75_03780"
     CDS             complement(9882..10454)
                     /locus_tag="PKK75_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNZ82746.1"
                     /translation="MEKDKNKKDLSSELSEQLLIILKARFEKNMNRHKDIEWSKVQEK
                     LKKNSDKMWALNEMEQTGGEPDLVGYDNKTDEYIFFDCSAESPQGRRNSCYDPEALES
                     RKKDKPESSAVGMAAQMGIEILTEEEYRELQKLGVFDIKTSSWIKTPDKIRKLGGAVF
                     CDRRYDTVFIYHNSAESYYGSRGFRGSLRV"
     gene            10709..11092
                     /locus_tag="PKK75_03785"
     CDS             10709..11092
                     /locus_tag="PKK75_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNZ82747.1"
                     /translation="MKYKAPSRCPVCGNKLSVTKLSCNKCSTTIEGDFHPCEFCSLPE
                     EDLDFVKVFIKCRGSIKDVEKELGISYPTVRSKLDAVIKSLGLEAKEPYIENEKIKAE
                     KSEILNQLSRGELSPKEATERIKNL"
     gene            11107..11550
                     /locus_tag="PKK75_03790"
     CDS             11107..11550
                     /locus_tag="PKK75_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82748.1"
                     /translation="MDDQKKVLEMIEKGQITAAEGMELLNALNKSAEEQNDNSVTAYT
                     SVKKTYKFLKIRVTSDNNSVNVNVNIPIRLLGVFGGLAGKMTKMVPRDAREEMESKGI
                     DITSIDFEKIIEELMNGTLDDPTIVDIEAWDEEHSTTVKVKIYAE"
     gene            complement(11649..12107)
                     /locus_tag="PKK75_03795"
     CDS             complement(11649..12107)
                     /locus_tag="PKK75_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNZ82749.1"
                     /translation="MSYLLQIVETEKQPVLSVKTTTSVSNMPNVVGRAYSSIVNYIME
                     KGEEPVGPAFIAYYNMDMENLNVEIGFPVTKEIEGKDDIVLRYIPAGKKATGFHKGSY
                     GEIGPVYEQLTKFISEKGYKPTGVTYEYYYNAPDEIPESELLTKIEFLLE"
     assembly_gap    12118..12217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12318..14168
                     /locus_tag="PKK75_03800"
     CDS             12318..14168
                     /locus_tag="PKK75_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ82750.1"
                     /translation="MKYDAIIIGGGLSGLTAASLLAKRNLKVAVIEKSYNPGGSCGIF
                     KRGNVTFDQGSAMLYGFGENGFNAHNFVFNCLEEPIDMINHDLLYIVNFNGHRIKFWP
                     DIDKFAEELSKVFPDEKENIKHFYNDMLRIYSHVLVETPTYTSADETDIRSAMKSVSK
                     HPISYIKFLSFLNKSAKNLLEKYFKNPEIFKFFDKLTSTYCYATVEEAPAVLAAVMFV
                     DNHAGGSYYPAGSTIFLPGKLEKVIEENNGDMFLESKAVKILFENDKPVGVLLKDGRN
                     LRADNIIYSGTAWNLYEKLIDSEYTTHEERQWVKNQVPTYPSVVLYSVVDKEVIPPET
                     APIEMLVGRPDKIDESEVTAYIFSIDDRTICPEDVHTVVAIGPSFESWDYDADYKNSQ
                     DYKLKKEKERKRLISVLEKRFPGFEASLRYAEIATPCTIQRYTMKNGGAVAGPKQMLG
                     QHMLKRQHIRTKWDNLFCCGESTVMGTGTPAVTTSGLSAANAVLKKYGIEPFAYREGM
                     KNYVRIVDKPFTNDQLFSNYDEKTRIIMKKAMRCRLCEHPSCSRDLDVRGIMRRTAVG
                     NFSGANKIWLNNKTDAESMEKYENNCICKHEEGNPVEIRDVIKYISGVKA"
     gene            14165..15724
                     /locus_tag="PKK75_03805"
     CDS             14165..15724
                     /locus_tag="PKK75_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ82751.1"
                     /translation="MSNEYDVVVVGGGIAGLTCAAYSCKYGYRTLLLEKSNNTGGLVK
                     TFRKNGYAFDGGIRAFENTGIVLPMLKNLGIDIEIVSNPVSIGIENKWARLESRESLK
                     AYSSMLAVIFPENRKEIDQIEREIKKVMEYMDVIYGIDNPLFRDDFNDKEYLVKTLFP
                     WLLKYQYNIGKAKRLSEPAGAYLKRITINQALIDMVIQHFFAETPTFFALSYFGLYLE
                     YFYPKGGTAVLVDKIADYVTASGGDIITNAGVTKINTEKNQVFVSEKIYSYKKLVWAA
                     NQKSLYEAIEKQESIKINNQRELVSKGITNDSILTVFLGVNKGNEYFMGICGAHAFYT
                     PSYQGLSSIGSWKNEIRSKEHLYHWIEKFLELTTYEISCPALRDASLAPYGKTGVIIS
                     TLMDYNLVKHISDTYDYEDFKDFCTKKIIGVISKTILPEIQNNIEMSMCATPLTIEHL
                     TSNKDGSITGWSFANKYMPSENNFKKITKSIYTPIDNIYQCGQWTFCPSGLPVSVLTG
                     KCAADEIKKAL"
     gene            complement(15770..16024)
                     /locus_tag="PKK75_03810"
     CDS             complement(15770..16024)
                     /locus_tag="PKK75_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82752.1"
                     /translation="MSDKHKKSNDKYNIQWDINSLNASSSTDLTGLIPFGPDSDEELK
                     AYNEILTYRPENTVAEDNEKSMNQTLHARRTTTDFKNKKG"
     gene            complement(16136..16612)
                     /locus_tag="PKK75_03815"
     CDS             complement(16136..16612)
                     /locus_tag="PKK75_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ82753.1"
                     /translation="MKYKLPLIVVDDMAVSRKFYEEVLNQRVIIDYGANITFSGDFAL
                     QTKESWVNFIRKSENDVLFKSNNFELYFEEDQFDEFVEDLKSFKVQYVHNVIEYPWGQ
                     RVIRFYDPDMHIIEVGERMETVVRRFIGQGLSIEETAERTQQPVEFVKACYESSSR"
     gene            complement(16649..>17138)
                     /locus_tag="PKK75_03820"
     CDS             complement(16649..>17138)
                     /locus_tag="PKK75_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82754.1"
                     /translation="KMQTINDYINEYTIQLRKGQIQKAYKGIMTFMSELKNYMAGRHP
                     EYAASALYFGYMDMTYFAFTPVELKKKNLKIAVVYLHEQNRFEVWLGGSNRKVQAEYI
                     ELLRKMDIGCYKLSQTGPGVDSIIEVQIDDRPDFEHAADLMQKIEDKTIELSNNIISI
                     LT"
BASE COUNT         5622 a         3026 c         3015 g         5375 t
ORIGIN      
        1 agcatttggc agcatagtcc ctcagtgata gtatcatatt tcattttatc aagataagca
       61 ttcggttttt tcttttttgt ttctgttaaa aaccgttcaa ataattcatt tgttaaaaaa
      121 tccggctgtc ggatcataat agagtaagca aagttgcttt tatcagctat tgatttgctt
      181 ttatcaacca aatcccacac tccctcaaga ggaaataccg tataatcgta aaatcctcca
      241 ggtatgacct tgcttttata agacatcctt accgcataac taaagctgta aagagcagct
      301 gtcgcatgtg aaaattcttc tccgttggga tctccccggc cgtcaataat tgcaaacctt
      361 atttcgggta cttcaatgat acaaggactg ctctttggca gatataaatc cttaaaatcc
      421 tttttgtaat caaccttatt catttgatac tcctatatgt atgtttttca tatctttaaa
      481 tttaactccc tcattgcttt ccttcaaacg gccgatgact taagatgccg gatacaagct
      541 atcctgttta tatttctata gtcaggccca ctcccacctc ttcaacagga atgaatttat
      601 gtatttcggc tttagcagca aaagaaacac aatggcaggg ataaagcctg gatatcttgt
      661 ttctcaaaaa atactcaata gtctttttca actgatcaga cacatcaaac agatgaaatc
      721 cccctattac tccgattacc cgttcttcac tgcagatttt ttttgcatgt tcaataatgc
      781 tgcatatgcc gttatgggaa cagcctgtaa taatgaacaa tcctccgccg ctgttataaa
      841 caagggctgt atcttctatt atatagtcag gttgttcttt gtcttcagca gatactgtac
      901 ctattgcttt tcttgtttca aaatcattat aaggaggtat ctcacctaaa aatgttatat
      961 tagggctgat cctgaccggt tcctttgtca gtgtcacttt gcacagcttc tttaattcct
     1021 ccaatgaaag tattgagcct atttcttctc catcacagta tttcctgtcg aaagcatttg
     1081 gatgggctat aatatttttg ccggcaattt tattttcagc agccagataa aaaaggcctc
     1141 ccgtatgatc attgtggccg tgagagatag caatagcagt tacagaatct aaatctatat
     1201 taagatcatg ggcatttttt ataaacatgc cggaatatcc tgtatcaaac aatattttgc
     1261 tttcatcgtc ttctatatag aatgatagcg caggttcacc aaaataatat ttatcaatat
     1321 aagtattatt atctattaaa acagtcagtt tcataaatta cctcataaat tattatcttc
     1381 tttatatgat cttacaataa tggaaataga cataggctaa tatgaaccat cccttattgt
     1441 atcccacagt ttgacattta gaaatcgcga cagggtttgt gcatctgttc tgatatttaa
     1501 taggttgccg tgagaaagaa cattaatcct atcaccatca ttcatgacca ggtttaattc
     1561 gtaggaataa taagaatgat ccattccatc cgaatcttgg ctcgaaacaa aacttgacaa
     1621 aagctgtatt gcataaatgt catcaagtaa tatgcatctt ttgttttgtt ttttagagtc
     1681 atttaagtcc gtcttccttc ttcccttgta aaacaagccc gaccttttat caaaaacaac
     1741 aggcctagta aatttaaaca gcccataaag acctaccgcc ataaaaagaa tcggtattat
     1801 tactaaaaat ttacttcctc taaatggagg gcgcatgaaa attaatggaa acgcaacaga
     1861 tactaaaaca aatgctgaga aaaatattat tacgcctgga gttggtcgga atttaataac
     1921 caattcgtcg gtttgaacca atctgccggt gcaaaagttt gctcctcttg catttggcag
     1981 agggctccat tctgttatca tagcaaccgg gtcattaaac cgtcttgggt ccacttcaac
     2041 cggattatta atcttgttaa ttattttatt gaatttacta ataatattat ttataatttc
     2101 catttttatc tctccattca tatactattg acttatcctt attatctatt cttcatccta
     2161 agtatcagcc ctggttacac tttcaacata cattatttca catgcaccat taccggtatc
     2221 atttctggca agactgcttt tccagtcata gtcagggaac ttcacattaa ccaaatatcc
     2281 cttcagtgtt atataatctt ccttatgaac ttgtatcagc ttcaataata ctttattatc
     2341 ctttggaatt atatgtacat tggctgaatg cttaagtatg taatcaccgt caacacaact
     2401 ttggcagcca tacctgtaaa aatacatctt cctgattgac tgtagtttat actgtcatcc
     2461 atttccttag tatcaagctc gccccatact agcagcaagt caatgggtga gacttcagaa
     2521 gcagaatcaa agaaataata cctcttgctt ttgacaactg ctgaaatttt atattcagct
     2581 aatttttcca tttttattgt cttattattc ccatcgtgat ttatttcatt attaaatcct
     2641 gtttttatat caacttgtac tggttcacct atcatcataa cttctttgtc atttaagccg
     2701 ggcaggataa taaatgtaat aactgctata attaaaattt caattaaaaa tattactaat
     2761 tcctttttca ttttaaacct gcctttcaca gacagagcag acagatgaaa ggtccgtccc
     2821 cgtgtcttta ctacagatga aacgtatcca tgtcttattc atatttatat ttaacaacaa
     2881 ataattccaa tggctcatct cccttattgc ctaattcagt aataacgcct ttgggaatat
     2941 tgaccagccg gcctgcacca aaggtttttt attctgttat atcagcaaac agaaacggaa
     3001 taatccttct taggtcatcc ggtattattt caatctgata tccaatcttt ccggcactga
     3061 caaaaattgt atcctttcct gatgcacttt catccacaac ggtagtaaat ctttttttca
     3121 tgccaatcgg tgaacatccg ccgtgtatat aaccggtaag cggcagcagt tcctttgcct
     3181 taatcatctc cacagacttc tcattcaccg tctttgccgc tttgcgcaaa tccagttccc
     3241 tgctgacagg gaccacaaaa acataatgct cgcctgacct tccgacagca acaagtgtct
     3301 tgaatacacg gtctatatct tgacccagca cagccgccac ttcaagaccg ctgaccgcat
     3361 cagtatctgc ataacaatgg tggcggtagg gtatcttttc cttatccagc agcctcatta
     3421 cattggtttt ctgctctcct tgtttttctg tatttttagc cataaatacc acctggtttc
     3481 attctgttgt aagctttttt cttaaatcat tgattaatca attatatact aattttttgc
     3541 aatcctcaac gaattttgta aggctttttt atataatcaa ttttatttat catatatacc
     3601 cttgtgatta tttaataaac aattatattt aactgtaaaa aacttgcact tatgatataa
     3661 tgaattcaaa taataaagca aagacttcag tctttgccca gatgcattca ttgtatcagt
     3721 ttgatgcaag ccgaattggg gcttgcactc atgacgttat aacataaata aatatgatga
     3781 tagaatagga gtcaaatatg aaggataaca aaatcaaaat agctatactg ggatttggta
     3841 atgtaggtaa ggcctttgcc aaacttctga ttgaaaaaga agaggaaatt tttgataaat
     3901 ttagtttaaa ggttgttgta tcggcaattt caaccaagtc aaagggctgt ttaattaatg
     3961 aaaatggtat tgatttggaa ggtgcccttg gggatttgga aaagttcaat catttcgatg
     4021 aaaaaagatg tgactacaat gaaatgagtt caatggatgt agctgaaaat gcagactacg
     4081 atatactttt tgagctgact cccctaaaaa tattttccgg tcagcctgca attgatcaca
     4141 tatcttctgc cctgaagagg aaaaaacatg cagtcagtgc aaacaagggg ccgatagcct
     4201 gggcatataa gcctttaaag gaattatcaa agaagaataa tgttttattt tattatgaaa
     4261 caactgtgat ggatggcacg ccggttttca acctggttga tgaaaccctt aaattctgta
     4321 aaattctaaa gatagaagga atactgaata cgactactaa ctttgtttta gaagaaattg
     4381 caaaaggcaa gtcatatgat gaggctattg cggaaggcaa aagaaggggt tttgttgaag
     4441 aagacccggc aatggatatt gaaggatggg atgcagcggc aaaaactgca gctttattaa
     4501 atgtgctcat gaatgcggat ataactcctt acgatgtgga cagaaaggga atagaggatg
     4561 ttacattgga gcaaataaag gaagcagaat caaggggaaa tgtaattaag ctattgtgct
     4621 acggcaaaat agaagacggt aaagtaattg ccagagttca accagaagaa atttctaaaa
     4681 attctctgct gtcaaacatt aacggaacaa cttcaatagt cagcattact acagatctta
     4741 tggctaccgt atcaattata gaacatgagc cggaaataga acaaacagct tatggagtgt
     4801 tcagcgattt aataagagta ttaagcaata tgaaatgttt gtaatgctgt attatttgct
     4861 gcactgaaac aaatatcatc aaggatatct tagaatatgg agatgtgaat atgaaaaaat
     4921 tcactttttt cactataatt gtattgttat tggctttgat tatggtttcc ataaacattg
     4981 aagaaataat aaatatattt atagaaaagc aggaacagca tgaggaaatt ataaataaag
     5041 attctattaa aaattatttt cccataaggc aaaacataaa atatgtctat gaagcaagca
     5101 gcgatgattt gctttatgaa gtcacgcctg attacacaag cgagggcatg attcagctca
     5161 gaataaccgg aaatgaggga gtgactgttt ctgtaattaa agtgaatgat aaaaaggcaa
     5221 ctggcagaat aatgatgcaa gaaaaccttc ttagagagaa tctcctaaat tcagcaaaat
     5281 tacacaatca agacgacatg gaggatgtcc ttttgatgga acctctggca acgggtactg
     5341 aatggactgt atcaggcaat catcaaagaa aaattacgaa tactgactca atagttgaaa
     5401 ctgatattgg aacatatgat gccatagagg taacaacaga atcagtaaac tataaaaaag
     5461 ttgattatta cactaaaggc ataggtctca taaagtcggt cataacatca gaagacaaaa
     5521 caacaacaat tgtaattaca gaaattattg aaaattctcc ttttacagaa aatattgaat
     5581 tttattatcc ggactttaat aaaatgaaaa tattgtataa ggttcaaaat gtagaattta
     5641 aaacaaatga ccttactgaa acagtaatcg cagaagctta taaaaaagct ttgccgaaag
     5701 aaaacagtgt tttaacaaaa gaaaccggaa taaacaggat tttttgggat gcaggcggaa
     5761 ccctttgcat tgatttgaac aaggcattta ttgacaatgc aaaaaagaaa gccctccatg
     5821 aaggaatggt cctgcagagc attgcaaata catttggcag atttttcaat acggaacagg
     5881 tttattttac tgtagatgac gaaaattatg cgtctgacca tatagattta agccaaggga
     5941 cttttgctgt taattttatc ggttaccctt tattttatga tattgtaata tatggaggca
     6001 ccccgtcagg aataaccgcc gccgtctctg ctgcaagaga gggaatggat gttgcattaa
     6061 ttgaacaagg cagtcatata ggcggtatgg tcacaggagg actgggtttt acagaccggg
     6121 gtaacactaa aattatcgga ggaatcacca gggaggtttt tgaaaaaata ggcagacact
     6181 atggcaagga tgcaggatgg gactttgaac ctcatgtggc agaaaatgtg ctgctggaaa
     6241 tcattgaaaa agaagaaatc gacttatact ataataaaaa aataaaggaa gacaatggaa
     6301 ttgaaaagga tggtaaaaaa attatcagta tagaaacgga tgatggagat ttattttttg
     6361 cacaggtttt tattgacagc tcatatgagg gtgatttgat ggctatgtcg aatgtttcat
     6421 atactaccgg cagagaggac agcaaaaagt acaatgaaag ctttgccgga ctgcttcctc
     6481 ccatgggcac aaacaatttc tattataatc tcaatgcaca tgatgaggac ggcagcctgt
     6541 atagtggcat tttaaatgaa tatcccggtg ctataaacag gggggacaag aagatacaag
     6601 cttataatta cagattgtgc gtaacaaacc aaccggaaaa tcgaataccg ttttataagc
     6661 caaataatta taatgaggaa aactacaagc ttttagctgc ctggctgaat aaaatcaagg
     6721 agatagaaaa ccggaacctg gccttttcag atgtggtata tttgggaagg ctgccaaaca
     6781 ataagtatga cgtgaacaat tacggccctt tttcaacaga tttaatcgga ggaagctggg
     6841 aatatcctga tgctgattat gaaaggcgtg aagaaataaa aaatgaacac aaggagtata
     6901 tacaaggtct cttgtatttc atttccactg attcaagtgt tcctgccgaa cttcgtgaag
     6961 atgtgaaaca atggggttat gctgcagatg aatttgaaga taatcattac tggccttacc
     7021 agatttatgt aagggaaggc agaagaatgg ttggagatta cgttttaacc gaatcggata
     7081 ttcgtaaaga aaaaaccaaa tatgactctg tcggcatggg ttcttacagc atagacactc
     7141 ataatgtaca aagatttttg accaaggacg gatttgtgct taatgaaggc gagcttcaat
     7201 accctgtaac accctatgag attccataca gggtgatgat tccaaaggct gcagaggcag
     7261 ataatttaat cgtatccgtg tgcatttcag ccagccacat agcttattca tccgtgagaa
     7321 tggaacccca atacatgatt atgggtgagg ctgcaggatt agcagccaaa atttcaattg
     7381 atgataatac aaatgtacaa aatattaatt acaataaatt gaaagcgaag ttaaccgaga
     7441 atgtagctgt gctaaggttt gaataaaact tgtcaccata cctgctccaa ttgtaaatgc
     7501 cctccaaaga ctatatcaat ggaaggcctg aatataacaa ttccggcaat tatcagccgg
     7561 atttatctat gcttacaata attgccttga agttgattag ccgaattcat tcaaaaacgc
     7621 tggattattt ggctaatatt tcagctgttt atagggctcc aagtgggctg ttacctgtac
     7681 attaccgtta atttccatcc ttattgtttc ttcgatgttg tgtattagct catgggactt
     7741 ttctacactc atttccggtt ctgtcattat atgcatatct ataaacaaag cgttgccgta
     7801 tcccctgctt ctaatattat gtgcgttttt tacctgctcg tagcttagca caatagtttc
     7861 aattttacta ttgtcaattg ccgccttatc caacaatata ccgctattat ctctaaaaat
     7921 ttcatatgca gcatgcagga taaatcctgc aacagcaaag gatgctatag aatcaataat
     7981 agcaggcaag ccaaacctta tacaagttaa agttataagt actccaatcg atacataaat
     8041 atcacttctt gtgtgcattg aatcagagat tagtatctga ctgttaaatt tccttccttt
     8101 tgcgtattca tatttgctta taaaaatatt tacacatagg gtaactacta aaacgataag
     8161 ggattctatt gatactttag gaataatcgg attcataatt ctttctgctg tattgaaaat
     8221 tactttaaac cctataaaaa acagcattcc tgctataaaa agtccggcga gcatttcaaa
     8281 ctttccatgt ccgtatgggt ggtcttcatc cacaggttta gacgcaaagt aaataccaat
     8341 taatccgact atattcgatg atccgtcaga taaggaatgg aaaccatcag cagtcatact
     8401 actacttttt acaatgctgc caataacaat ctttaataaa gctacaccta agttagcaat
     8461 taatataatc aataaaaccc ttttgacatt cttaaaacta tttgtcaaag ttctacctcc
     8521 taacaatgat taagttataa aaaatctgtg aaactatttt atagtagtcc cacagattca
     8581 atttctgctg ccaatcggcc cggcttatga cattaagaaa tgtcctgctt gttcttatta
     8641 tatacaatta tatatacaat atatgtatct tacctttttg ggttacagtt atttatttta
     8701 ataataaatg aggtcagaca taacatggac accctgtccg gaataatcct tataatgcat
     8761 tccataagat ttttacccat attctttccc ctgtgccgtt cttttaatgt tcttttggtt
     8821 tacctaaatg ggtaacgcct attcagataa ttatatgtaa agtctgcaaa taatgaatac
     8881 ataaaacagt acacgattga aaaggccatt aatactggta tttgaatcag tatgctgccg
     8941 ggaaaattaa cacccaggac aggaattcca attagaaaat ttactatggc aagcgaaaat
     9001 gttagaataa ctccgcttaa tatggatatt ataatttttg attttgtaat ataactaaag
     9061 gctcctattg aaaagcccac taatccatta gtaaataaaa aaaacatacc ttcatgtgaa
     9121 ttaaacgtat aaattaatac accagtaatt atataagctg taaatccagc ctttggattt
     9181 gaccttgaca aaatatatat aggtactgca cttaatattg tggcaataat aaatatttct
     9241 ccaaaggctg caggcagcaa ttgaagcaag acagataggc agatcagcaa tccgccttga
     9301 gttatatttc taatgctcaa acacaacacc gccaattata ttattagtta atatatatga
     9361 cggctatatt tgttatgttc cacgtaatct cctaaattat caatttctta ggtctaacct
     9421 tgaagcaaat gtgctgacgg aactgccctt tgccgataaa agctttgatg tttcaattac
     9481 ttcttttgtt gcacacggtc ttagtaaaaa tgaacggcaa gtaatgtatg atgaaatgat
     9541 aagaattaca aaacatctgg ttataattta tgattacaat gaacacaggg ggttaattac
     9601 cgatttagca gaatggcttg aaggcggcga ttattttaac ttcattaaaa caataaaaaa
     9661 cgaattaaat gataaatttg aagatttgac aataattaat gccaatatac gttcagcttt
     9721 gtatatagca aaattgtagc ttattttaca taattaatat tatgtaaaca aaaagagatg
     9781 tttaaataat gttatataag ccggaagtga cacatgatgt gtgtccctta actatactta
     9841 atataacata ataaaaatta tgtaaagtaa gactaatttt attatactct taatgaacca
     9901 cggaatcccc ttgagccata gtatgattca gctgagttat ggtatataaa gacagtatca
     9961 tagcggcggt cgcaaaaaac tgctcctcca agctttctga ttttatcagg cgtttttatc
    10021 cagcttgatg tttttatatc aaaaactccc agcttctgca gctccctata ttcttcttct
    10081 gttaatattt ctattcccat ctgggcagcc atgccgactg cactactttc cggtttatcc
    10141 ttcttccttg attccaaagc ctccgggtca taacaagaat ttcttctgcc ttgtgggctt
    10201 tcagcagagc agtcaaagaa aatatactca tctgttttat tgtcatatcc cacaagatca
    10261 ggttctccgc ctgtttgctc catttcatta agcgcccaca ttttatctga atttttcttt
    10321 agtttctctt gtactttcga ccattctata tctttatgac ggttcatatt tttttcaaaa
    10381 cgggctttta aaattataag cagttgttcg ctaagctcag atgacaaatc tttcttgttc
    10441 ttatctttct ccatatggat atttcctcct aaaaaataca gtatataatc ttttatagaa
    10501 atacccactt ttctaaatat taaacaatat tcttatgaag ccatccttgc tgcaaaacta
    10561 tcctccctca tctccccctt caggtcccat ttattaatat tattaatttt ataattaatt
    10621 tttttaattt tgatattaat ttttttaatt ttcatattga catttaatga aaaatggtat
    10681 atgttgttta taaaaggaag gtgagttaat gaaatataaa gcaccaagca gatgccccgt
    10741 atgcggtaat aaactttcag ttacaaaact tagctgtaat aaatgttcta caacaatcga
    10801 aggagatttt catccttgcg aattttgcag ccttccggag gaagaccttg attttgtaaa
    10861 ggtatttata aaatgccgtg gaagtattaa ggatgtggaa aaagagttag gaatttcata
    10921 tcctacggtt cgaagcaagt tggatgcagt tataaagagc ttagggcttg aagccaaaga
    10981 accatatata gaaaatgaaa aaataaaagc agaaaaaagc gaaatactta atcagctgtc
    11041 cagaggagaa ttatcgccta aagaggcaac agaaagaata aagaatttat aaaaggagaa
    11101 caggaaatgg atgatcaaaa aaaggttttg gaaatgattg aaaagggtca gattactgca
    11161 gcggaaggca tggagttatt aaatgcgctg aacaaatctg cagaagagca gaatgataat
    11221 tctgtaacag catatacgtc tgtaaaaaaa acttataaat tcctcaaaat aagagttaca
    11281 tcagataata attcggtaaa tgtaaatgtt aatattccaa tacgtttatt aggcgttttt
    11341 ggcggattag caggcaaaat gactaaaatg gtgcctaggg atgcccggga agaaatggaa
    11401 agcaagggta tagacataac aagcattgac ttcgaaaaaa ttattgaaga attaatgaac
    11461 ggaacgttag atgatcccac catagtagat atagaggcat gggatgaaga acacagcaca
    11521 actgtaaaag taaaaatata tgctgaataa acctatacac tttatattat tccatcaatt
    11581 aaaaggatag ctgtgacagc tatcctaatt ttttgaatca gtatgaccct gtttcttttt
    11641 ttaattcatt attcaagcaa aaattcaatt tttgttaaca gttcgctttc aggaatttcg
    11701 tccggtgcat tgtaatagta ttcataggtt acacctgttg gcttatatcc cttttcgctt
    11761 ataaattttg ttaactgctc ataaacagga ccgatttcac cataagaacc cttgtggaat
    11821 cctgtagcct tcttgcccgc aggaatgtat cttagaacga tatcatcttt gccttcaatt
    11881 tcttttgtaa caggaaaacc tatttcaaca ttaagatttt ccatatccat attgtaatag
    11941 gctataaatg caggcccaac cggttcctcc cccttctcca taatataatt tacaattgag
    12001 ctgtaggctc tgcctactac attaggcata ttgctgacgg atgtggtagt ttttactgat
    12061 aatacaggct gcttctcggt ttcaacaatt tgtaacaagt aactcataaa agcctccnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg tacaataatt tcagcagata
    12241 ttgcaaagtt aataaaaatg aaaaattctt ctaagcgggg atctgtattc tgtaattaga
    12301 aataaggagg agtgtttttg aaatacgatg caataattat tggaggaggt ctctcaggtc
    12361 ttacagctgc aagtcttctt gcaaaaagaa atctaaaggt tgctgtaatt gaaaaaagct
    12421 ataatccggg aggttcctgc ggtatcttca aacgcggcaa tgtaaccttt gatcaggggt
    12481 cggctatgtt gtacggcttt ggcgaaaacg gatttaatgc acataatttt gtgtttaact
    12541 gccttgaaga gccaattgat atgattaatc acgacctttt atatatcgtc aattttaacg
    12601 gccatagaat aaaattttgg ccggatattg acaagtttgc agaggaattg tccaaggttt
    12661 ttcctgatga aaaggaaaat atcaagcatt tctataatga tatgctaagg atttatagtc
    12721 atgttttagt tgaaactccg acttacacat cagcagatga gacagatata agatctgcca
    12781 tgaaaagtgt ttccaagcat ccaatatctt atattaaatt tttaagtttt ttaaataaaa
    12841 gtgcaaaaaa cttattagag aaatatttta aaaatcctga aatatttaaa ttttttgata
    12901 agcttacatc gacatattgt tatgctactg tagaagaagc tcctgctgtt cttgcagctg
    12961 ttatgtttgt ggacaaccat gcaggaggaa gctattatcc ggccggctca acaatttttt
    13021 tgcctggaaa gctggaaaag gtaattgaag aaaacaatgg tgatatgttt ttggaaagta
    13081 aagctgtaaa aatattgttt gaaaatgata aacctgttgg agttttgctg aaggatggaa
    13141 gaaacttaag ggcagacaat attatttatt caggtactgc ttggaatttg tatgaaaaat
    13201 taatagattc tgagtataca acccatgaag aaagacaatg ggtgaaaaat caggttccta
    13261 catacccaag tgttgttttg tattctgtag ttgataaaga ggttatacct cctgaaactg
    13321 cccctattga aatgctggtt ggaaggccgg ataaaattga tgaaagcgaa gttacagcat
    13381 atatattcag tattgacgac agaactattt gcccggaaga tgtgcataca gtagtagcaa
    13441 taggtccatc ctttgaaagc tgggattatg atgcagatta taaaaactca caagactata
    13501 agctgaaaaa agaaaaggaa aggaaaagat taataagcgt tctggaaaaa aggttcccgg
    13561 gttttgaagc atcgctcagg tatgctgaaa tagcaacacc ttgcactata cagcgttata
    13621 caatgaaaaa cggcggtgca gtagcaggac caaagcagat gctaggacag catatgctga
    13681 agagacagca tatacgcact aaatgggata atctcttttg ctgcggcgag tctactgtca
    13741 tgggaacagg aacgcctgca gttactacat caggactatc tgcagctaat gctgtgctta
    13801 aaaaatatgg catagagccg tttgcatacc gtgaaggaat gaaaaactat gtaagaatag
    13861 ttgacaagcc atttacaaat gatcaactgt tttcgaatta tgatgaaaag actcgcatta
    13921 taatgaaaaa agccatgaga tgcagacttt gtgaacatcc tagctgttca agagatctcg
    13981 atgtacgagg catcatgcgc agaactgctg tagggaattt ttcaggtgca aataaaatat
    14041 ggcttaataa caagactgat gcagaaagca tggaaaagta tgaaaataac tgcatctgca
    14101 agcatgaaga aggtaatccg gttgaaatca gagatgttat caagtatata agcggggtga
    14161 aagcgtgagt aatgaatatg atgtagtagt tgttggagga ggcattgcag gacttacctg
    14221 tgctgcttat tcatgcaaat acggatatcg tacacttctc ttagaaaaat caaacaacac
    14281 gggaggcctg gttaaaacat tccggaaaaa tggctatgct tttgatgggg gtatccgggc
    14341 ctttgaaaat acaggaattg tcctgccaat gctgaagaat cttggtatag acattgaaat
    14401 agtaagcaat ccggtatcta tcggcataga aaacaaatgg gcaaggcttg aatcaagaga
    14461 aagcttaaaa gcttatagct caatgctggc agtcatattt ccggaaaaca ggaaggaaat
    14521 cgatcagata gaaagagaga ttaagaaagt catggaatat atggatgtaa tctatggaat
    14581 tgacaatcca ctatttcgtg atgattttaa tgataaagaa tatcttgtca aaacactttt
    14641 tccctggctg ctaaaatatc agtataatat tggaaaggca aagcgtttaa gcgaaccggc
    14701 cggcgcttat ctaaagcgca ttaccatcaa tcaagctctt attgatatgg ttattcagca
    14761 tttttttgct gaaactccaa cattttttgc tctaagctac ttcggcctct atctggaata
    14821 cttctatcct aaaggcggta ctgctgttct tgttgataaa atcgcagact atgtaaccgc
    14881 atcaggagga gatattataa caaatgcagg ggttacaaag ataaataccg agaaaaatca
    14941 ggtttttgtt tctgaaaaaa tttattctta caagaagtta gtttgggctg caaatcaaaa
    15001 gtccttatat gaagctatag aaaaacagga atccattaag attaataatc aaagagagct
    15061 tgtttcaaaa ggaatcacta atgattcaat acttacagtt tttttagggg taaacaaggg
    15121 caatgaatac tttatgggta tatgcggtgc ccatgcattt tatactcctt cataccaagg
    15181 cctttcttca atcgggtcat ggaaaaatga aataaggtca aaggagcatt tgtaccattg
    15241 gatagaaaaa ttcctggagc tgaccacata tgaaatatcc tgtccggccc taagagatgc
    15301 aagccttgct ccttatggaa agaccggagt gattatcagt accttgatgg attacaacct
    15361 ggttaagcat atctctgata catatgacta cgaggatttc aaagatttct gcaccaaaaa
    15421 aataatagga gtaatcagca agacaatact tcctgaaata cagaataata ttgaaatgag
    15481 catgtgtgcg actccgctga caattgaaca tttgacatca aataaagatg gttcgataac
    15541 aggatggtca tttgcaaaca aatatatgcc ttctgaaaat aatttcaaaa aaattacaaa
    15601 atcaatctat actcctatag ataatatata tcaatgcgga cagtggacat tctgtccttc
    15661 cggtttgccg gtatctgttt taacgggaaa gtgtgctgca gatgaaataa agaaggcatt
    15721 atgaaaaaat cccttgaatt ttttcattca agggaaacag cgctttgaat taaccttttt
    15781 tgtttttaaa atctgttgtt gttctccttg catgcaaggt ctggttcata cttttttcat
    15841 tatcctctgc aactgtgttt tcaggtctgt aggtcagaat ttcattatat gcttttaatt
    15901 cttcatccga atcaggtcca aatggaatta atcccgttaa atctgttgat gaacttgcat
    15961 ttagagaatt tatatcccac tgaatattgt atttatcatt tgatttttta tgtttgtcac
    16021 tcatgctgaa tctccttact atgttttatt tatagtatgt ataaaaagag aaattttaaa
    16081 caaaatttcc ccctgcattt tttcaagggg aatttatttt cgaaattttc atttttcatc
    16141 tgcttgaaga ttcataacat gcctttacaa attcaacagg ctgctgtgta cgctctgctg
    16201 tttcctcgat tgacaatccc tggccgataa accgcctgac tactgtttcc attctttcac
    16261 caacttctat tatatgcatg tccgggtcat aaaaacgaat tactctttgt ccccatggat
    16321 attctatcac attatgaaca tattgaacct tgaaggattt taggtcttct acaaattcat
    16381 cgaactggtc ctcctcaaaa taaagctcaa agttattgga tttaaacaaa acatcatttt
    16441 ccgattttct aataaaatta acccatgact ccttggtttg cagtgcgaag tcaccagaaa
    16501 aagtaatatt tgctccataa tctattatta ccctttggtt taaaacttct tcataaaact
    16561 ttcttgatac tgccatatca tctacaacta tcagcggcag cttatacttc atataaatta
    16621 tcctcctttt ctcagctatt ccctcttact aagttaagat tgaaatgata ttatttgaaa
    16681 gctcaattgt tttatcttct attttctgca tcaaatctgc agcatgttca aaatccggcc
    16741 ggtcgtcaat ctgtacctct attatagaat caacccccgg gcctgtctgg gaaagtttgt
    16801 aacaacctat gtccattttt cttagcagct ctatatactc agcttgcact tttctgttgc
    16861 tgccgcctaa ccatacttca aatctatttt gttcatggag atatactacc gcaattttta
    16921 agtttttctt ttttaattca acgggtgtga aggcaaaata tgtcatgtcc atgtagccaa
    16981 aatacagggc acttgctgca tattcaggat gcctgcccgc catataattt ttaagttctg
    17041 acatgaatgt catgatgccc ttatatgctt tttgaatttg accttttctt aactgtatcg
    17101 tgtattcgtt tatataatca tttatcgttt gcattttc
//
LOCUS       DAOPUT010000025        17128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3392_length_17128_cov_19.372167, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000025 DAOPUT010000000
VERSION     DAOPUT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17128
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3392_length_17128_cov_19.372167"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1908
                     /locus_tag="PKK75_03825"
     CDS             61..1908
                     /locus_tag="PKK75_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82755.1"
                     /translation="MNYYEEESYSSFNIKPWLKLSRFFKPYRRKIIGLVLFMFLTASM
                     DLSIPLFQKYAVDNFIVRDTLEGINGFIALYIGALVTMALSVTIFARLAIEVEMHIGK
                     DLRKEIFEHLQNLSLSYYNTTPVGYILARTLSDTNRIGAMIAWGLVDVFWSLAYVIGA
                     FIAMFMLNSRLALVVGMVIPIMALITVYFQNKILSASRKLRKVNSKMTGSFNEGITGA
                     KTSKTLVIEDKNLFEFKDLSSEMKFTAVRLARLNALYIPIIIFFGSVLTALVLARGGR
                     LAMEQIIQIGTLSAFLSYAVNIFEPIQQLARTLAEVISLQANIERVTDLLDQEPLIKD
                     SEDVVKIYGDNINPKKENWEEIKGEIEFKNVTFKYPDGDEEVLKNFNLHIPAGTNIAI
                     VGETGAGKSTLVNLACRFFEPTGGQILIDGKDYRERSQLWLHSNIGYVLQSPHLFSGT
                     IKENIRYGKLDATDEDIIKAAKIVSVDIIINKLENGYNSNVGEGGDRLSTGEKQLISI
                     ARAILADPKIFVLDEATSSVDTNTERLIQKGIEYLLKGRTSFIIAHRLSTVKKADIIL
                     VVNDGKIIERGTHKGLIAKRGHYYNLYTKQFEEEKTLELFGEIPSANKD"
     gene            2066..2329
                     /gene="ffs"
                     /locus_tag="PKK75_03830"
     ncRNA           2066..2329
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKK75_03830"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            2450..2974
                     /locus_tag="PKK75_03835"
     CDS             2450..2974
                     /locus_tag="PKK75_03835"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNZ82756.1"
                     /translation="MDDRKVKEMVYTALFAALVCVATFIIKIPLPVSGGYVHLGDGFI
                     FIAVILIGKKNGAWAGAIGASLADIIGGYSIYALPTFIIKGIMALIMGLMTEKLPFSV
                     KRKWIAGAITGSVWQVISYYLVGSLMVGNFLTTISEIPGNVAQSAAGIVVAAAFLAVF
                     KHTTIKGTGTHLLM"
     gene            complement(3062..3448)
                     /locus_tag="PKK75_03840"
     CDS             complement(3062..3448)
                     /locus_tag="PKK75_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82757.1"
                     /translation="MFRNFLAGRYGVDHLTRAMLIAGIVLTFLGNAFDLYLFTLLSYL
                     MFFGSVYRTTSRKFDVRRNENNKFLQYYKPAKLWVINKYKIASSSRYYKYFNCPSCNS
                     QLRVPRGKGKISVTCGKCNHKFLQKS"
     gene            complement(3462..4832)
                     /locus_tag="PKK75_03845"
     CDS             complement(3462..4832)
                     /locus_tag="PKK75_03845"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNZ82758.1"
                     /translation="MRGFVNLLIVGLIGLLSSTYLFNLSPVDYRGTIEIEAPVEESTL
                     SLKVEEDKILNPESIAISHTASKEEIVRIVADSIFTVDIYKDNKLIKSSIDNLEPVSP
                     SIDATISVNEDNQMITALNISQKNLGLEDGIYEFIFNSNLISDEDRSFVSVTVTYDTP
                     GSYYPAVNKAPAGTKGLTLYFPTVDAETVIPVTRFVVEDKSITRMAVEQLQNGPLNKG
                     MTTVIKDVTNTTYNNGNVVIDIPSSYDGYNSGSTGSMMAYNSFVKTIFAVNRYWPIHN
                     LTFTVDRRNVDTYFHGMSSEFINRLPNTEKKYILYLAHKTEDDRYYLFEYQLYPDQLG
                     IFENDTIEIKAEKMFGAYQNTDISYGSSPVPKTVALKTVSLQGRTIVLDFNKELLSVY
                     KDKNDLRHMMVESLMYSFTTIPGVDSIRITAEGEEISDFIEGLDTKKVLYPPEFINPE
                     TVETQQ"
     gene            5139..5426
                     /locus_tag="PKK75_03850"
     CDS             5139..5426
                     /locus_tag="PKK75_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82759.1"
                     /translation="MNKRNYDRDMMEKMYDIIELIYILKRLTDDFYEDDRSKQYLLEM
                     MNDMQNVKYSIDKYKGTGIKRNASKFDYRHYGTGDTFIDKIIFYLKSLNEG"
     gene            5489..5698
                     /locus_tag="PKK75_03855"
     CDS             5489..5698
                     /locus_tag="PKK75_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82760.1"
                     /translation="MFGNILGDSGDSLLFFFLLLVVLACFGGDDGLIGGIGGFGDNDS
                     LLFFFLLLVIIFCSCDKVGGIGDRC"
     gene            complement(5775..7754)
                     /locus_tag="PKK75_03860"
     CDS             complement(5775..7754)
                     /locus_tag="PKK75_03860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNZ82761.1"
                     /translation="MENLKLKDFLDYTFLSGLELSPDKNQAAFVVHTSDFEDNKYLSN
                     IWLFNCTTLEYKKLTSLNEEKSFIWLDNNTILFPSLRDEKLEKRIKEGEYWTVFYAID
                     IRGGEAYEYMRIPMKVKSIKKIADEKFLLTAAYDHYDINLHSHKGEEKTKAVDLIKEN
                     KDYEILDEIPFWANGEGFTNKKRNRLYLFDKGTKDILPISNQFENVSVTSIKSGKALY
                     VSSSYTNKMELTTGLFMYDLYNSQALCLVEDLEYYIEFAEFVGDDIVVAATAMDKYGI
                     NQNFNFYKLQNGELEFLCRHDYGLYNTTGSDCRYGAGELKRSAHNCLYFISTENKSSF
                     VKKLSLDGNIEKLTADIGSVDCFDICSEGMIFIGLRGGSLQEIYTLQNNVETQITKFN
                     EKIKETKTIVKPETLNFDNYGVNIEGFVLKPVNFDENQSYPGILDIHGGPKTVYGEIF
                     YHEMQYWANEGYFVFFCNPRGSDGRGDEFADIRGKYGTIDYDDLMKFTEKVLEKYPQI
                     DRSRIGVTGGSYGGFMTNWIIGHTDRFKCAVSQRSISNWISKFGTTDIGYYFNADQNL
                     STPWDNVEKMWFHSPLKYADLAVTPTLFIHSEEDYRCWLAEGLQMFTALKYHGVDARL
                     CMFRGENHELSRSGKPRHRVRRLEEITNWFEKYLK"
     gene            8012..8950
                     /locus_tag="PKK75_03865"
     CDS             8012..8950
                     /locus_tag="PKK75_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNZ82762.1"
                     /translation="MDNFIFSLNVVLPLVLLMLIGVFLRKIKIFDEFFLNKLSIFIFK
                     VSMPFLLFNNIYTGKISDTVDPYFVILALILVLGTIGILIMVIPKYVKENRNRGVLIQ
                     GLYRSNFILFGIPLSVNIFGDEGLAAVTTLVAIIIPVYNFAAVIVLDLFTDHRQNYKE
                     TAVSILKNPLIIGTVLGILTSVFQIKLPAVLDKTIADIAKTATPMALMALGGEIEINN
                     IWKNIKYLILVSAGKLFIIPAVTILIAFFLGYRGAELCAFLCMMAPSTAVSSYTMAQQ
                     YDCNHELAGQIVFFTTIISPFSIFLFVFMLKTTGLF"
     assembly_gap    9293..9392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9527..9901
                     /gene="gcvH"
                     /locus_tag="PKK75_03870"
     CDS             9527..9901
                     /gene="gcvH"
                     /locus_tag="PKK75_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNZ82763.1"
                     /translation="MKLLQELKYVESHEWVRLEENTAYVGITDYAQDKLGDIIYVEVP
                     QAGTVVEAGDQFIVIESVKAATDVYAPVSGTVVEVNEELEDNPALINESAYDAWIVAI
                     DMSMPVEADSLLSAEEYEELCE"
     gene            9980..10806
                     /gene="guaA"
                     /locus_tag="PKK75_03875"
                     /pseudo
     CDS             9980..10806
                     /gene="guaA"
                     /locus_tag="PKK75_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
     assembly_gap    10807..10906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10920..11876
                     /locus_tag="PKK75_03880"
     CDS             10920..11876
                     /locus_tag="PKK75_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNZ82764.1"
                     /translation="MKIVILDGYTENPGDLSWEGFERLGELTVYDRTPMEKTIERIGD
                     ADIVLVNKAPVTRETLEACKNIKYIGVLATGYNIVDIEAAKEKGIPVSNIPTYGTDAV
                     GQFAIALLLEICHHIGHHAQVVKEGRWENNDDWCFWDYPLIELSGKTMGIIGLGRIGR
                     TTGKIAKALGMKVIAYDDYKNDEGREIAEYVGLEQLFKYSDVICLHCPLFPSTQGIIN
                     KDTIAKMKDGVIIINNSRGPLIVEQDLADALNSGKVYAAGVDVVSSEPIKPDNPLLSA
                     KNCIITPHISWAPKESRQRLMDIAVDNLKAFINGAPINIVNK"
     gene            12072..13214
                     /locus_tag="PKK75_03885"
     CDS             12072..13214
                     /locus_tag="PKK75_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNZ82765.1"
                     /translation="MMKNKKLVMIPGPTPTVRSINDQMGRETVAFGDPAFVKDFSELI
                     VDLKQMWRVDGECFVVAGSGTMAMEMAVANVTKRDDNILIVSNGFFGDRFIDICTRKG
                     LNVDVISAEWGDVVSPEAVEEKLKEKNYAAITVTHVDTSTGARAPIEKIGEVIKKYPN
                     TVYIVDGVAASAGEREYVDDMNIDILFTGSQKAFGVAPGLAIVWAGKKALARRKSLGT
                     IPEYFIDFEKWIPIMNDPSKYYATPAVNMVWALKESVRLIKEEGLEERYERHTRVSNA
                     FNDSLEAMGFKVLAKKEHRCSTLSTVLYPEGINDAEFRKVLAEEGAMVAGGLAAYAGK
                     MFRIGHMGNIDDHTVYSAIAAIERTLIRLGYKYEKGIGLKTLLEAM"
     gene            13446..14762
                     /locus_tag="PKK75_03890"
     CDS             13446..14762
                     /locus_tag="PKK75_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ82766.1"
                     /translation="MSKPVLSAHFESRVPSDVRLSQMKYAEREVKPEAVINVAIGNVS
                     LPTNPAMQKRLFNLDAPESPFNKGVIRYTATGGFDETQAAFKNILKCEGFDTSKLSVI
                     VTDGGSTGMELLLIGVCGPAGTGEKPLMMIDPAYTNYISFAERIGRKTVTVKRYLNED
                     GKFSLPEIDKIEETIKANNPGALLVIPYDNPTGQLYDKETLVQLAKLCVKYNMWMVSD
                     EAYRELFYVEGSELVSIWGLTDKDVPGIEGRRISIETASKVWNACGLRIGAVITDSPE
                     FANRCAAEYTANLCANAIGQYIFGALAHESQVEIAQWCKSLRDYYGGLIKKVTAELKV
                     LEPSLVVSSPDASIYTVLDVRNIVKPGFDAIEFCLFSAGKGAVDIDGVQTTLLIAPMK
                     GFYDIAKGDANPGSTQFRIAYVETPENMMKVPKLFVELIRQYEASR"
     gene            15132..16118
                     /locus_tag="PKK75_03895"
     CDS             15132..16118
                     /locus_tag="PKK75_03895"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007058915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNZ82767.1"
                     /translation="MKYIENNSTDPHYNLAFEEYIFKNLDKEDFVLLWRNGPSIIVGK
                     NQNTIEEINMDYVKDNNINVVRRVTGGGAVYHDLGNLNFSFITKSQSNLIDFKTYNIP
                     IVRALEKLGVDCELSGRNDIVIDGKKFSGIAQSIIKERVLNHGTLLFDSKLDILSKAL
                     NVKRDKIESKGVKSVSSRVTNIKPYVKDEIDVLKFKELLLKNIFEYFNQPVEIYELSH
                     EDQENIQRMVDEKYGTWEWNFGYSPEFNFKGYKRFEGGGVEVRLHVVKGRIENCKIYG
                     DFFGKGDIKDLEDKLKGVKYDINQISEELEDVSIDQYLGRITKEEFMKCLFN"
     gene            complement(16182..16634)
                     /locus_tag="PKK75_03900"
     CDS             complement(16182..16634)
                     /locus_tag="PKK75_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNZ82768.1"
                     /translation="MKKILIPIDGSPLSVKVGESAVKLGKLINAELIFITVINMPSEE
                     KYAHFGMTVENQFSANRKEMLNKLMQQESRMLNIIVRNLDTVDLKVTQKLLVGRSAAE
                     ILKFAEEEYVDYIFMGRRGFSPVQRFFVGSVTQKVISAAPCPVIVING"
     gene            complement(16659..17099)
                     /locus_tag="PKK75_03905"
     CDS             complement(16659..17099)
                     /locus_tag="PKK75_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNZ82769.1"
                     /translation="MKKILVPVDGSAASQKAAAQAFSLARKYGSEVVVFHASIEHDID
                     MYNKFGVVIDPDMSKLKNKLKESESKMLDSVIKNFDIAGIDLKKKIVSGVPYEEILKE
                     AEAGKYDLIVIGQRGYSKIKRFFVGSQTVRVISDAPCPVLVVHE"
BASE COUNT         5569 a         2887 c         3336 g         5136 t
ORIGIN      
        1 cagggatggc atttataagg acatattcaa tgtccagatg aacctgtcgg aggacttata
       61 atgaattatt acgaggaaga aagctacagc tcatttaata taaagccctg gttaaaatta
      121 agcagatttt ttaaacctta caggagaaaa ataataggtc ttgttttgtt tatgtttctc
      181 actgcgtcaa tggatttatc cattcccttg tttcagaaat atgcagtgga taattttatt
      241 gttagagaca ctctggaagg gataaatggt ttcattgctc tatacattgg ggctttagta
      301 actatggccc tatccgtgac tatatttgcc cgtttggcta tagaagttga aatgcatatt
      361 ggcaaggatt tgagaaagga aatctttgaa cacctccaaa acctttcatt gtcatattac
      421 aacacaactc ccgtgggcta catactggca agaaccttaa gcgataccaa tcgaatagga
      481 gctatgattg catggggatt ggtggatgta ttctggtccc ttgcttatgt aataggtgca
      541 tttatcgcta tgtttatgct taactccagg ctagcacttg ttgtaggaat ggtaatacca
      601 ataatggctc ttataactgt gtattttcaa aataagattt tatctgccag cagaaaatta
      661 agaaaagtga attcaaaaat gacagggagt ttcaatgaag gaataacagg cgcaaaaacc
      721 tccaagacat tggtaataga ggacaaaaac ctttttgaat tcaaggatct aagcagtgag
      781 atgaagttta cggctgtaag attagccaga ttaaatgctc tttacattcc cattataata
      841 ttcttcggtt cggttttaac agctttggtc ctggcaagag gtggcaggct tgcaatggag
      901 cagattattc aaattggaac cctttctgct tttctttctt atgctgtaaa catatttgaa
      961 cctattcagc agctggcgag aacacttgcc gaagtcatat ctctccaggc aaatattgaa
     1021 agagttaccg acctgttgga ccaggaaccc ttaataaaag acagtgaaga tgtagtgaaa
     1081 atttacggag ataatattaa tcccaaaaag gaaaactggg aagaaattaa gggagaaatt
     1141 gaatttaaaa atgttacctt taaatatcct gacggagatg aggaggtttt aaaaaatttc
     1201 aatcttcata ttcctgcagg caccaatatt gccattgtag gtgagacagg tgccggaaaa
     1261 agcaccctgg taaatcttgc atgtagattc ttcgagccaa cgggagggca aatacttatt
     1321 gacgggaagg attacaggga aagatcccag ctttggcttc actccaatat tggttatgtg
     1381 cttcaatctc ctcacttgtt ttcaggaacc ataaaggaaa atataagata tggcaaactg
     1441 gacgcaacag atgaggatat tataaaagca gcaaaaattg tttccgttga tattataatc
     1501 aacaaacttg aaaatggtta caacagcaat gtgggtgaag gaggggacag gctttctaca
     1561 ggagagaaac agcttatttc aatagccaga gcaatattag cagatcctaa gatatttgtt
     1621 ttggatgaag ccacatcttc agttgataca aatacggaaa gacttattca aaaagggatt
     1681 gaatatttgt tgaaaggcag aacttcattc ataattgccc acagactatc aactgttaaa
     1741 aaggctgata ttattttggt tgtcaatgat gggaaaatta tcgaaagggg aacccacaaa
     1801 ggccttatag ctaaaagagg tcattattac aatttataca ccaagcagtt tgaagaggaa
     1861 aagactttgg aactatttgg ggaaattcct tctgccaaca aagactaagt acggggacat
     1921 tccttcatgg atttacttca caggcttact taccatggag gtgtgtcccc cttccatttg
     1981 atagaagaag gagtatcccc atacttgctt ttgtcaccaa attattacat tgacacaaat
     2041 tttttattat gttattattt ttatgctgtg ctacacgggg aggcagcggt gccctgaact
     2101 tgcaatccgc tacagcaagg tggaattccc tctccgaggc ttgcattgtg aggcctgcct
     2161 ttagtaagtg gcgttgataa cttggtcttg cgcaacaaaa acctatgaac cctgccaggt
     2221 ccggaaggaa gcagcagtaa gtagacaatt ttgtgtgacg cgagggtgcc gggtttgagt
     2281 taactgctga agtaacgctt gttatgcatt gtcgaaggag ggtgcacagc atttttaatg
     2341 cttttttatc tgtcatctaa acaaattgcc aatctgtatc ttttaaagag tacagaatcg
     2401 ggtataattt aatcagctta ggatatagat tttttggatg gtgataataa tggatgacag
     2461 aaaggttaaa gaaatggtgt acacagcttt gtttgctgca ttagtctgtg ttgcaacatt
     2521 tattataaaa attcccttgc ctgtttcagg agggtatgtt cacctaggag acggatttat
     2581 atttattgct gtaattttaa tcggtaagaa aaacggcgca tgggctggtg caataggggc
     2641 atctttagcg gacataatcg gtggatacag tatatatgca ctgcccacat ttataataaa
     2701 gggtataatg gctttgatta tgggactgat gacagaaaag cttccttttt ccgtaaaacg
     2761 taagtggatt gcgggtgcaa taacgggaag tgtgtggcag gtaatttcat attatcttgt
     2821 gggttctcta atggtaggaa acttcctgac aactatcagt gaaattcccg gaaacgttgc
     2881 tcaatcagct gcaggcatag tagttgcagc agcattttta gcagtgttca agcatactac
     2941 cattaaaggt accgggacac acctccttat gtaaaggaca tgaggcaagt cagaatgaat
     3001 gcccctgaat ttctatgttt cattgttaat aaggaacggt cattttatct aaaatgaccg
     3061 tttatgattt ttgtaaaaat ttatggttgc atttaccgca ggttactgat attttacctt
     3121 ttcctcttgg taccctcagc tgactgttgc agctcgggca gttgaagtat ttataatatc
     3181 tgcttgagct tgcaatttta tacttgttta taacccataa ttttgcaggc ttgtagtatt
     3241 ggagaaactt attattttca tttcgtctca catcaaattt tcttgatgtt gtcctgtata
     3301 cacttccaaa aaacataaga tatgacaata aagtaaataa atataaatca aatgcattgc
     3361 caaggaatgt taatactatg cctgctatga gcatggctct tgtaaggtgg tcaactccat
     3421 atcttcctgc caaaaagttt ctaaacatat cattctcctc tttactgttg tgtttctacc
     3481 gtctcaggat ttatgaattc aggcggatat aaaacttttt tggtatccag gccttcaatg
     3541 aaatcagata tttcttcgcc ctctgcagtt attcttatgc tgtcaactcc cggtatggtg
     3601 gtgaaagaat acatcaagga ttcaaccatc atatgcctta agtcgttttt atccttgtaa
     3661 acacttaaaa gttctttatt aaagtcgagt acaatcgtcc ttccctgaag ggatacagtt
     3721 ttaagcgcca cagttttagg tacagggctg gagccgtatg atatatctgt attttgatat
     3781 gcaccaaaca ttttttctgc ctttatttct attgtatcat tttcaaaaat accaagctgg
     3841 tccgggtaaa gctgatactc aaacaggtag tatctgtcat cctctgtctt atgagccaaa
     3901 taaaggatgt attttttctc tgtattaggc aggcggttta tgaactcgct ggacatacca
     3961 tgaaagtatg tatccacatt tcttctgtca acagtgaatg taagattgtg aataggccag
     4021 tatctgttaa ctgcaaatat tgtttttaca aaagaattat aagccatcat actgccagta
     4081 gagccgctgt tatagccgtc gtaggaactt ggaatatcaa ttaccacatt tccgttgttg
     4141 taagtagtat ttgtaacatc ctttatcact gttgtcatac ccttgttcag agggccgttt
     4201 tgcagctgct caactgccat tcttgtaatc gatttatcct caactacaaa cctggtaacc
     4261 gggataacag tttcagcatc aactgtaggg aagtagaggg taagtccctt ggtgcctgca
     4321 ggggctttat taaccgccgg ataatatgag cctggagtat cataggtaac tgtaacagat
     4381 acaaaagacc tgtcttcatc tgatattaaa tttgaattaa atataaattc atatatacca
     4441 tcttcaagcc ctaagttctt ctgacttata tttaaagctg taatcatctg attgtcttcg
     4501 tttactgaaa tagttgcatc tattgaaggt gaaacaggtt ctaaattatc aatgctgctt
     4561 ttaatcagtt tattatcctt gtatatgtca acagtaaaga tgctgtcggc aacaattctt
     4621 acaatttctt ccttagaagc tgtatgactg attgctattg attcaggatt cagtatttta
     4681 tcctcttcaa ctttcaaact taaagtactt tcttcaacgg gagcttcaat ttcaattgtt
     4741 cctctatagt caacaggtga aagattgaac aaataagttg atgataacaa gcctataagt
     4801 cctactatca ataaattaac aaatcctctc ataaaagcac ctcttatata tataatccca
     4861 aaagccatct tttgagccag tgattcttca agccgggcct tcaaatgaca acaccaaaac
     4921 atacctgcag cagttcggta tgaatatcgg tttaaatcat tatacaataa aaaatgataa
     4981 aatcaaagaa atatttatgg ccgtcccata ctgccgatga aagattgatt gagaacgtca
     5041 ggtaaccgac accaaataat ctccatataa tataatgagg caaatattcc tcctcttaag
     5101 aaatttcagc tgatgtttta atgaatggag tggtgagtat gaataaaaga aactacgaca
     5161 gagatatgat ggaaaaaatg tatgacataa ttgagttaat atatatttta aaaaggctta
     5221 ccgacgattt ttacgaagat gatcgctcga aacaatacct gcttgaaatg atgaacgata
     5281 tgcagaatgt caaatacagc attgataaat acaaagggac gggcattaaa agaaatgcct
     5341 caaaatttga ctaccgacat tacggtaccg gagatacatt catcgataaa ataatttttt
     5401 acctaaaatc attaaatgaa gggtaataaa gtaacaatta agagtctaag gaataatata
     5461 tactaaaatt attattagga ggttctatat gtttggtaat atattaggag acagcggaga
     5521 cagcttatta ttcttcttct tgcttttagt agttcttgct tgcttcggcg gagatgatgg
     5581 attgatagga ggaataggcg gctttggaga taatgacagc ttattattct tcttcttatt
     5641 gctggtaata atattttgca gctgtgacaa ggttggcgga atcggcgacc gctgctgata
     5701 aggaagggac tccatgtccc ccttaatgat aatacattca aaaagcttcc ctcgggaagc
     5761 ttttaaattt gcatctactt taaatatttt tcaaaccagt ttgttatttc ttccaatctc
     5821 ctgaccctgt gacggggttt gccgcttctt gacagctcat ggttttctcc cctgaacatg
     5881 cagagccttg catcaacacc gtgatatttc aatgcagtaa acatctgtaa tccctctgcc
     5941 aaccagcatc tgtaatcttc ctccgaatgt atgaacaaag ttggagtaac agcgagatcg
     6001 gcatatttaa ggggtgaatg aaaccacatt ttttcaacat tgtcccaggg ggttgacaag
     6061 ttttggtctg cattgaaata ataacctatg tctgttgttc cgaatttaga aatccagttt
     6121 gaaatactcc tttgggaaac agcgcattta aatctgtcag tgtgacctat aatccagtta
     6181 gtcataaaac ctccgtagga acctccggtt acaccgattc ttgacctgtc tatctgagga
     6241 tatttttcaa ggaccttttc tgtaaatttc atcaggtcat cataatctat ggtgccgtac
     6301 ttacctctga tatctgcaaa ttcatctcct ctgccgtcac tgccccttgg attgcagaaa
     6361 aatacaaagt atccttcatt tgcccaatac tgcatttcat gatagaaaat ttctccgtaa
     6421 acagtcttag gtcctccatg tatatctaaa attcccgggt atgactgatt ctcatcaaaa
     6481 ttaacaggct ttagaacaaa tccttcaata ttaaccccat aattatcaaa attaagagtt
     6541 tcaggtttta ctatggtctt agtttccttt atcttttcgt taaacttagt tatttgagtt
     6601 tcaacattat tttgaagagt atatatttcc tgcagactgc cgcccctcag tccaatgaat
     6661 atcatacctt cgctgcatat gtcaaaacag tccactgagc ctatgtcggc tgttaatttc
     6721 tcaatattcc catccagtga tagcttcttg acaaatgaac ttttgttttc agttgatata
     6781 aaatacaggc agttatgggc ggatcttttt aattcccccg ctccgtacct gcagtctgac
     6841 ccggttgtat tgtacaaacc gtaatcatgc ctgcaaagaa attctaactc tccattctgg
     6901 agtttataga aattaaagtt ttggttgatt ccgtatttat ccatggcagt agcagccaca
     6961 acaatatcat cacccacaaa ttcggcaaat tcaatatagt attccaaatc ttcaacaagg
     7021 caaagagcct gactgttata caaatcatac atgaaaagcc ctgttgtcaa ttccatttta
     7081 tttgtataac ttgaagaaac gtataaggct tttccgctct ttatgcttgt cacacttaca
     7141 ttttcaaatt gatttgatat tggcaaaata tcctttgttc ccttgtcaaa caaataaagc
     7201 cggtttcttt tcttgtttgt gaatccctct ccgttcgccc aaaaagggat ttcatccaat
     7261 atctcgtagt ccttgttttc ttttattaaa tcaactgcct tagttttctc ctctcctttg
     7321 tgagaatgaa gattaatatc gtaatggtca taggcagctg ttaagagaaa cttctcatcg
     7381 gctattttct taatgctttt tactttcatg ggaatacgca tatattcata tgcttcccct
     7441 ccccttatat caattgcata gaaaacagtc cagtactcgc cctccttgat tcttttttcc
     7501 agtttttcat ctctcaagga aggaaacaaa attgtattat tgtcaagcca aataaaactc
     7561 ttttcctcat ttaaggaagt aagtttttta tattccaagg tggtgcagtt aaacagccat
     7621 atgttggaca aatatttatt atcctcaaaa tcagaggtat gtaccacaaa agcagcctgg
     7681 tttttatccg gtgacagttc aagcccggat aaaaaagtgt agtctaaaaa atccttaagt
     7741 tttaaatttt ccatttaatc tttattacct cctttttagt gtctcccgta taattcaggg
     7801 aaattacttt aatccggaat tcattgggtc accgcattat taacaccatt atatatttaa
     7861 aagaacagtt gcgcaattaa aactattgtt taagaatagt tttgagagta taattgaaaa
     7921 ggaatcaaac aggaagattt gtcgtcctgt ggtacctgag cggatgaaat attttcttta
     7981 gccataatcg cataaaagga aggaatgaaa agtggataat tttatatttt ctttgaatgt
     8041 tgttttgcca ttggtattat taatgttaat aggagtattc cttagaaaaa taaaaatatt
     8101 cgatgaattt tttttgaata aattaagtat atttattttt aaagtatcca tgccattcct
     8161 tcttttcaat aatatttata caggtaaaat atcagatact gtagacccct actttgtcat
     8221 acttgctctt atactggttt taggaactat cggcatactc attatggtta ttcctaaata
     8281 cgtgaaagaa aacaggaaca ggggagtttt aattcaaggt ttgtatcgaa gtaatttcat
     8341 tctttttgga atccctctct ctgtaaatat tttcggagat gaagggctgg cggcagtaac
     8401 aactcttgta gccataataa tcccggttta taactttgca gcagttatag ttttggactt
     8461 atttactgat cacaggcaaa actacaagga aactgctgta agcatattga aaaatccttt
     8521 aataataggg acagtgcttg gcattttaac atctgttttt caaatcaaac ttccggctgt
     8581 acttgataaa actattgcag atattgcaaa aactgcaact cccatggcct tgatggctct
     8641 aggaggagaa atagaaataa acaatatatg gaagaatatt aaatacctga tccttgtttc
     8701 ggcaggaaaa ttatttatca ttcccgcagt tactatactg attgcatttt ttcttggata
     8761 caggggcgcg gaactatgtg cattcttatg catgatggca ccttctacgg ctgtatccag
     8821 ctacaccatg gcacagcagt atgactgcaa tcatgaactg gcgggtcaaa tagttttttt
     8881 tacaactata atcagtccct tcagcatttt tttgtttgta tttatgttaa aaacaaccgg
     8941 tttattttaa aatgtaccag tcattatact aaactgaaca ggatcttggt atcatctctt
     9001 gctgaaaaaa atggaatata tggatattat ttttacctat tgatattatt ggaataataa
     9061 taaaagtaga atagtcaata atattcatgt agatgcaaaa catcttcaaa tacatgtata
     9121 tgaagggatg atgggaataa aaaggttcag tttcagcaat aaaaacaatg atgataagct
     9181 aaagaattgg gaaaacaaag caaaattaga cagccaagaa aacttaaagg agcaaatgag
     9241 ccgaatgaat aacgaaaaca aaaataggaa cgaaagcaaa tttgaaaacc gtnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccgccgg ttttataata tatgcaaatt
     9421 aagcaataaa gaataatata gaacttgaga cagatatttc tgtccttaat aataagggga
     9481 cattccatac ttaaaagtcc aagaattttc acggagggaa ctaactatga aattactgca
     9541 ggaattaaaa tatgttgaat ctcatgaatg ggtgaggttg gaagagaaca cggcatatgt
     9601 aggaattacg gactatgcac aggataagct gggagatatt atttatgtgg aagtgcccca
     9661 ggcgggaact gttgttgaag caggagacca gtttattgtg attgaatcag ttaaggcagc
     9721 aacagatgtt tatgcacctg tctcaggaac agttgtagaa gtaaatgaag aactagaaga
     9781 caatcctgct ttaattaatg aatccgcata tgatgcttgg attgtcgcaa tagatatgag
     9841 tatgcctgtt gaagcagaca gcttgttatc agcggaagaa tatgaagaat tatgtgaata
     9901 aaaaaattta gaagaaatag gagtatgaaa gatgagaaaa gatttgaatc ccgagaagtt
     9961 tgttagcgat tcaattttac agataaaaaa tcaaattgga gataaaagag ccctttgtgc
    10021 cttgtcggga ggcgttgatt catctgtatc ggcagctatg gttcacaagg caataggcaa
    10081 gaatttaatc tgtgtatttg ttgaccatgg tcttatgaga aaagatgaag gggaccaggt
    10141 agaaaaagta tttaaagaca agatgaatat gaatttaatc agggtcaatg cccaacaaag
    10201 atttttaagt aaattagccg gtgtgactga acccgaaaga aagagaaaaa tcataggcga
    10261 agaatttatc agggtgttcg aagaagaaaa acagaagctt aaggatgaat tcgggcatat
    10321 tgactacctt gtgcaaggca caatctatcc ggatgttgta gaaagcggaa cctcttcttc
    10381 atcgctggta aagagccacc acaatgtggg agggctcccc gatgaagttg attttgaact
    10441 tgtagagcct ctcagactct tgtacaagga tgaagtaaga caagtcggaa gagaattggg
    10501 gattcctgaa gaaatagtgg gaaggcagcc attcccggga cccggccttg caataagggt
    10561 actgggagaa gtaactgagg aaaaattaag aatagtaaga gaagcagatt acatatttag
    10621 agatgaaata aaaaatgcag gtctgcaaaa caaaatatgg caatattttg cagcacttcc
    10681 aaacatcaga agcgtaggtg ttaaagaaga tgaaagaaca tataaacaca caataatttt
    10741 aagggcggtc cattcaacag acggtatgac ttcagactgg gcccgtatac cttatgaggt
    10801 gctggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagg gagaatatca
    10921 tgaaaattgt tattttagac ggttatactg aaaaccccgg agatttaagc tgggaaggct
    10981 ttgaaagatt aggcgaactg acagtatatg acaggacacc catggaaaaa actattgaac
    11041 gcattgggga tgcagatata gtcttagtca ataaggctcc tgtaacaagg gaaacactgg
    11101 aagcatgcaa aaatattaaa tacatagggg ttcttgcaac cgggtataac attgttgata
    11161 tagaagctgc caaggaaaaa ggaattcctg tttcaaatat tcctacatat ggcactgacg
    11221 cagtaggcca gtttgcaata gcactgttat tggaaatctg tcaccacata ggacaccacg
    11281 ctcaagttgt aaaagaaggc cgatgggaga ataatgatga ctggtgtttc tgggattatc
    11341 ccttaattga gctttccgga aaaacaatgg gaataatcgg acttggccgc atcggaagaa
    11401 caaccggaaa aatagccaag gcactaggta tgaaggtaat tgcatatgat gattataaaa
    11461 atgatgaagg cagggaaatt gcagaatatg tggggctaga acagcttttc aaatactcgg
    11521 atgttatatg tctccattgc cccttatttc cgtccaccca aggaatcatc aacaaggata
    11581 caattgcaaa gatgaaggat ggggttatta taataaataa ttccaggggt ccgttgattg
    11641 tagaacagga tttggcagat gcattgaatt caggaaaagt ttacgcagca ggagtagacg
    11701 tggtatcatc agagcctata aaacctgaca atcctcttct aagtgcaaaa aactgtatta
    11761 ttactcccca tatttcatgg gctcctaagg aaagccgcca gcgtctgatg gatattgcag
    11821 tggataactt aaaagctttt ataaacggtg ctccgataaa tattgtaaac aaataaatga
    11881 aaccttaatc agcaactttt gttgattaag gtttttttat gttatacttg ttcaattcgg
    11941 gatttaaata ataaaaattt tcagttgata aaaatcggct aaagagatat tatattagtt
    12001 ggaaataaat tttttagcaa agccaggtta tatttataaa atactaagta ctaaactaat
    12061 aaataggagg tattatgaaa aacaagaaat tagtaatgat tccgggacca acacctacag
    12121 taaggtcaat caacgaccag atgggaagag agacagttgc atttggagat ccggcatttg
    12181 tcaaggattt tagcgaattg attgttgatt tgaagcaaat gtggagggtt gacggtgaat
    12241 gctttgttgt agcaggcagc ggcacaatgg ctatggaaat ggcggtggca aatgttacaa
    12301 aaagagacga taacatattg atagtatcca acggattttt tggcgacaga tttattgaca
    12361 tttgtaccag aaaaggtctt aacgtagatg taatatccgc tgaatgggga gatgttgttt
    12421 ctcctgaagc tgttgaagaa aaattaaaag aaaaaaatta tgcggcaatt accgtaaccc
    12481 atgttgatac ttcaacagga gcacgtgcgc caattgaaaa aatcggagaa gtaataaaga
    12541 aatatcctaa caccgtatac atagttgacg gagttgcagc ttcagcaggg gaaagagaat
    12601 atgttgatga tatgaatatc gacatattgt tcacaggttc tcaaaaggca tttggagtcg
    12661 caccgggact ggcaattgta tgggcaggaa agaaagcact ggcaagaaga aagtctctgg
    12721 gcactattcc tgaatatttt attgactttg aaaaatggat tccaataatg aatgaccctt
    12781 caaaatatta cgcaactccg gcggtcaata tggtctgggc acttaaggaa tcagtgagac
    12841 taataaaaga agaaggcctt gaagaaagat acgaaagaca tacaagggta tcaaatgctt
    12901 tcaacgattc tttggaagca atgggcttta aggttttagc taaaaaagaa cacagatgct
    12961 cgacgctttc aacagtttta tatcctgaag ggataaatga cgccgaattc agaaaagtat
    13021 tggctgagga aggtgctatg gtagcaggag ggcttgcagc atatgcaggt aaaatgttca
    13081 ggataggcca tatgggtaat attgacgacc atacagttta ttcagcaatt gcagccatcg
    13141 aaagaacatt gatcagactg ggatataaat atgaaaaggg aataggcctt aaaacattgc
    13201 ttgaagcaat gtaatacaaa agttataaag cagcagaata caaagctgct ttaactttat
    13261 atcgggacga ttaaatattg aaattgatgc ataatacata cttgaaataa agtaaataaa
    13321 caaatggagc ctacatataa atatgtaaca aattatgtgg acaaaatctc agaagttggc
    13381 tataattata tgagatagag atttaattaa catacataac aatatattta ttaggaggat
    13441 tataaatgag taaaccagta ttgtctgcac actttgagtc aagagtgcct tcagacgtaa
    13501 gactttcaca aatgaagtat gcggaacgtg aagttaaacc tgaagctgtt attaatgtag
    13561 caataggtaa tgtatcatta cctacaaatc cggctatgca aaaaagactg tttaatttag
    13621 atgcacctga gagcccgttt aataaagggg taatcagata taccgctaca ggcgggtttg
    13681 acgaaacaca ggcagcattt aaaaacatct taaaatgtga agggtttgat acaagcaaat
    13741 taagtgttat agttacagac ggcggctcaa ccggtatgga gctgttatta atcggcgttt
    13801 gcggacctgc aggaaccggt gaaaaacctc tgatgatgat tgaccctgca tatacaaact
    13861 atatttcttt tgctgaaaga ataggacgta agactgttac agttaaacgt tatttaaatg
    13921 aagatggaaa gttcagcctt ccggaaatcg acaaaatcga agaaacaata aaagccaaca
    13981 accctggagc attgcttgtt ataccttatg acaaccctac aggccagctt tatgacaaag
    14041 aaactcttgt tcagcttgca aagctctgcg ttaagtacaa tatgtggatg gtaagcgacg
    14101 aagcttatcg cgaactcttc tatgttgaag gaagcgaact ggtaagcatc tggggactta
    14161 cagataagga tgtgccgggt attgaaggaa gaagaataag cattgaaact gcatcaaagg
    14221 tatggaatgc atgcggattg agaataggtg ccgttattac cgacagtcct gaattcgcta
    14281 atcgctgtgc tgctgaatat acggcaaatc tttgtgcaaa tgctattgga cagtatatat
    14341 tcggagcatt ggctcatgaa tcacaggtag agatagccca gtggtgcaaa agtctccgtg
    14401 attactacgg cggacttatt aagaaggtaa cagcagagtt aaaagttcta gagccctcct
    14461 tggttgtttc aagccctgat gcatccattt atacagtatt ggatgtgaga aatattgtaa
    14521 aacctggatt tgatgcaata gaattctgtt tgttctccgc tggaaaaggt gctgtagata
    14581 tagatggtgt tcaaacaact ttattgattg cacctatgaa gggattctac gatatagcca
    14641 agggtgatgc taaccccgga agcacacaat tccgtattgc atatgttgaa actccggaaa
    14701 acatgatgaa ggttccaaaa ctgtttgtag agctaattag acagtacgaa gcaagcagat
    14761 aataatttag aaattttagg gtggtttctt cttttgggag aaccacccct tatttttaca
    14821 cctattaatt ttaattctat tctatttaat catatttttt tcatacctct atatattatc
    14881 cgctcattag tttcggggta tttaacatta ggaactgcca tcaacactac agctaatatt
    14941 aaggatatag tcttattaat cattatgtca ccaccttctt tagtatatta tacatttagc
    15001 caaaatcgaa ttcaaacttt tttcagaaca gaaagaaccg tccccaaaaa ttaattgaac
    15061 tggataaata tctattatat aataggtaaa atatatttac ataattttaa ttatgtatac
    15121 tggaggatag aatgaaatat attgaaaaca attcaacaga ccctcattat aatttagcat
    15181 ttgaagagta tatttttaaa aacctggata aggaagattt cgttttattg tggagaaatg
    15241 gaccctcaat aattgtaggc aaaaatcaaa ataccataga agaaataaac atggattatg
    15301 ttaaagataa taatattaat gttgtaagaa gagtaaccgg cggcggagca gtttaccacg
    15361 atttggggaa cttaaatttc tcctttataa ccaagtcgca aagcaattta atagacttta
    15421 aaacatacaa tattcctata gtaagagcct tggaaaagct gggagtagac tgtgaattgt
    15481 ccggcagaaa cgatattgta atcgacggaa agaaattttc aggcatagct caaagtatta
    15541 taaaggaaag agttttaaat catggtacct tgctttttga ttctaaatta gacatattgt
    15601 ctaaagcatt aaatgtaaaa cgggataaaa tagaatcaaa gggagtaaag tccgtgtcaa
    15661 gcagggtaac gaacattaag ccctatgtta aagatgaaat tgatgtattg aaattcaaag
    15721 agctattatt aaaaaatatt tttgaatatt tcaatcaacc ggttgaaata tatgaattat
    15781 cccatgagga ccaggagaac atacaaagaa tggttgatga aaaatacggg acatgggaat
    15841 ggaattttgg atattctcct gaattcaact ttaaaggata caaaagattt gaaggcgggg
    15901 gagttgaagt gagactccat gtggtaaaag gaagaattga aaactgtaaa atatacggtg
    15961 acttcttcgg caagggggac attaaggatt tagaagacaa attaaagggt gtaaaatatg
    16021 acataaatca aataagtgaa gaattggaag atgtatcaat agaccaatac ctgggcagga
    16081 ttacaaagga agaattcatg aaatgcttat ttaattaatt tatgatgaaa agcaggggac
    16141 actgatagtg tccccttacc atgcattctt cttttaaaaa cttatccgtt aattacaatt
    16201 acagggcatg gcgctgcaga aataaccttt tgggttaccg agcctacaaa gaatcgttgg
    16261 acaggcgaaa atcctcttct acccataaag atataatcaa catattcttc ttctgcaaac
    16321 ttaagaattt ctgctgcaga cctgccaacc aataatttct gagttacctt taaatcaacc
    16381 gtatctaagt ttcttacaat aatgttaagc attctagatt cctgctgcat taacttgtta
    16441 agcatttcct ttcggtttgc tgaaaattga ttctcaacag tcataccgaa atgtgcatat
    16501 ttttcttctg aaggcatgtt gataacagtt atgaaaatta attcagcatt gattaacttg
    16561 cctaatttta cggcactttc acctactttg actgataagg gcgaaccatc aataggtatt
    16621 aaaatttttt tcataataac ctccatatat ttatatattt attcatgtac aaccaagacc
    16681 ggacacggtg catcagatat gacgcgtact gtttgagagc ccacaaagaa acgtttaatc
    16741 ttcgaatatc ctctttgccc gataactatc aggtcatatt ttccggcttc tgcttccttt
    16801 aggatctcct catagggcac acctgaaaca atttttttct ttagatcgat acctgcaatg
    16861 tcaaagtttt taataacaga atccaacatt ttgctttcac tttcttttag tttgtttttt
    16921 agtttactca tgtcgggatc aattacaact ccgaacttat tgtacatgtc aatatcatgc
    16981 tctatggaag catgaaaaac tactacctcg ctgccatatt tccttgcaag gctaaaggcc
    17041 tgggctgcag ccttctgaga agcagctgag ccatccaccg gtactaatat ttttttcata
    17101 agcttcctcc tcattaataa aatataat
//
LOCUS       DAOPUT010000026        16820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3479_length_16820_cov_19.178586, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000026 DAOPUT010000000
VERSION     DAOPUT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3479_length_16820_cov_19.178586"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..966)
                     /locus_tag="PKK75_03910"
     CDS             complement(250..966)
                     /locus_tag="PKK75_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HNZ82770.1"
                     /translation="MEYLIIIVIITFILSLPSVKGFFGEIFIRFLLKLLDKEKYIVVN
                     DVLIPTSDGRTSQIDHVIISIYGIFVIETKNYNGWIFGSEHSRNWIQVIYKTKNQFYN
                     PIIQNNGHVKAIQDILSDYSSIRYIPIVVFTLKANLKKLDVSSHVVYSLGLLNTIRLY
                     NDENISQVDLERIHQIIINANNEDAVARMEHVERINNFKYKVEAGICPRCGGRLVERN
                     GKYGEFVGCSNYPKCRYVFK"
     gene            complement(976..4125)
                     /locus_tag="PKK75_03915"
     CDS             complement(976..4125)
                     /locus_tag="PKK75_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HNZ82771.1"
                     /translation="MSLNNGFYESVINNLIDEEIHKLRHQKEYYIDKKQIDEEESSSI
                     LSKYMSIVINKSLNRISGKEKLSKQIDICNKIINLLIDELELHDFNEFIISENAELLL
                     AVLNSINKSPLEKNHKIRPVTSIAMNALFTGAKNEPTLGSELVKEINTAERIDMLVSF
                     IKWSGLVQIYEALKDFTKEHELRIITTSYMGATDYKAILELSKLPNTEIKISYDTKRT
                     RLHSKSYMFYRNTGFSTAFIGSSNLSNAALTSGLEWNLKISEYTSKEMLNKFNATFES
                     YWHDNEFYSFNKDNENDTKTLINALKVESNTDSNGSQFYFDITPYSYQKEILDKLQAE
                     REVHNRYKNLLVAATGTGKTIISGFDYKRFVEDNPGSKNKLLFVAHREEILKQSLDAF
                     RMILRDRNFGDLWVGRYEPSQIEHLFISIQTFNSKEFTENIKEDYYEYVIIDEFHHSA
                     AKSYRKLLEYVKPKILLGMTATPERMDGKDVTKYFDNKIAAEIRLGEAINRKILSPFN
                     YFAVTDEVSLKDIEWKSGGYIVSELEKVYTKNNQRANLIIDSIKKYTKSIKDVRALGF
                     CVSKLHAEFMCKYFNKVGIPSIYLTSDSSNEERDSAKSKLNKKEVNFIFVVDLYNEGV
                     DIPAIDTVLFLRPTESITVFLQQLGRGLRLYDGKDCLTVLDYVGQANKSYNIYEQKFR
                     ALIGNTNFTVEKEIGNEFPNLPKGCFIQLERIAKDYILESIKNVINNKTNIKRKLIEY
                     ASNKSTYDVVEFCKSSNVELTDIYKSYSFTQLLAEAGVIKDFDYNQYKKESDGISRLL
                     HINSKEYIEFILKILMTNKFDYKSLNDYEELVLNMFYYELWQDTLEKSGFDKIKDCID
                     KLKGCPHLLNEIISILEYNLENIDFKEKDINLGYPCPLKLHSRYSMAEVLAALEEYKL
                     GYKAPFWQGVKYLDEKKTDIFFVTLNKSEKDYSETTMYDDYAIDDECFHWQSQSVTSD
                     ISPTGQRYINHEKLGSKVVLFLRERKSVNGITEPYYCLGTAKFVSYEGSKPMSIVWKL
                     DNKMPEFIKKKTSGNVMVG"
     gene            complement(4324..5697)
                     /locus_tag="PKK75_03920"
     CDS             complement(4324..5697)
                     /locus_tag="PKK75_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HNZ82772.1"
                     /translation="MMEQVVGSQMIFGLVVGIALLIVLILKTKIHAFLALIIAAAVTG
                     LVGGMPSNDVLSSISKGFGNTLGSIGIIIGFGVMMGEIFEASGAAERMAQTFIKKLGK
                     GKEEIALAITGFLVSIPIFCDSGFVILSPLAKAISRKTKKSVITLGVSLAAGLVITHS
                     LVPPTPGPVGVAGLFNVNVGSLILWGIVLAIPMTLAAVLYAKYLGKKIYQLPTEDGED
                     WIRPAYQEPDYSSILNEENENLPSTFMSFAPIVIPIILILINTVLTAMKASGTVVNAL
                     VFLGTPVIAVGIGLIVAIYGLSGKYTRNETIDLMERGIKSAGIIILVTGGGGSLGMVL
                     RDSGAGDYIANAISQTALPPILLPFVIATLIRLVQGSGTVAMITSASITAPIIATLNV
                     NPVFAALAACTGSLCYSYFNDSYYWVVNRMLGIKDAKEQMRVWSFTTTIIWAVGFVEL
                     LILNAIF"
     gene            complement(5721..6719)
                     /gene="pdxA"
                     /locus_tag="PKK75_03925"
     CDS             complement(5721..6719)
                     /gene="pdxA"
                     /locus_tag="PKK75_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNZ82773.1"
                     /translation="MSKPIIGIPLGDPAGIGPEIVVKALNEKEIYEVCSPLVVGDKSI
                     LEQAMKFCDISLNINSVKEPANGKYEYGTIDLIDLNNVDVNRLKIGKVQAMGGKAAFE
                     YIKTVANLALEKKVDAIATTPINKESLKAAEVPYIGHTEILEDLTNSYNPLTMFQVHN
                     LRVFFLTRHVSLRKACDLVTKDSVYEFITRCIEALRVLGIKNPKIAVAGLNPHSGEHG
                     LFGYEEVDEIEPAVKKAKEEGIDVVGPVPADSVFYFGLKGRYDAVLSLYHDQGHIATK
                     MVDFDRTISITNNMPFLRTSVDHGTAFDIAGTGKAGEVSMVEAIKLAALYAPNFNK"
     gene            complement(6722..8059)
                     /locus_tag="PKK75_03930"
     CDS             complement(6722..8059)
                     /locus_tag="PKK75_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HNZ82774.1"
                     /translation="MSKVIIIADDLTGANATGVLLARQGFKSATFLNLDKYDKEESKY
                     LDVISISTDSRAIHSDEAYERVKTIAEFFKDDDVALFSKRIDSTLRGNIGSEISGLLD
                     NLQEDTYALVVPAFPSSGRVCIGGFLMVNQVPLEKTDVAKDPKTPVYTSRVVSLLEEQ
                     TDKNVGFVELDKVLKGEFAIEQSLLSEIKRGCRIIVVDATTDEDIDRIAKAVKNSKLN
                     IISVDPGPFTAAVAREYLEVPAISPGKKVMLTIGSVSNLTRKQIDELRLEFTPYLVEA
                     DALKLIYEDTKEHEMERIINKLMDNMEDFEVIGVITTRNENQVLNLTEIANDLNIHEE
                     EVSLKITNGLAEITTNLLKKAEDQIGGLYTSGGDVTVAVCKALECAGIEVKDEVLPLA
                     VYGRLIKGKYHNTPIITKGGLVGDEKALIKCVNYLLTKLSNEYGAKNSKKEGE"
     gene            complement(8069..8830)
                     /locus_tag="PKK75_03935"
     CDS             complement(8069..8830)
                     /locus_tag="PKK75_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNZ82775.1"
                     /translation="MLQIERRQMIIQYLEEHEAVTVEELAKLFNVAPMTIRRDLQFLE
                     DKNIAARTFGGAVLKSRLIDEVPYENKSLANIEKKKKIADYAASLIRNGDTVILDAGT
                     TNMEIARLLVNYKNLKIITTDLNIATFVSASTDFEILFPGGKVQNSTGSCYGSSTVDF
                     LKNINADISFIGSSSVDIQHGITTPSMEKADVKRQMIKCSDKSILVADSSKFGKKSLI
                     KVCNLNDFHGIITDSNLDELTADKIKSKKIKIKLV"
     gene            complement(9097..9366)
                     /locus_tag="PKK75_03940"
     CDS             complement(9097..9366)
                     /locus_tag="PKK75_03940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ82776.1"
                     /translation="MEVKKGKNKFYIGIEEAPLAEIIFSINEEGIIVAESTKVSEELG
                     GKGAGKLILKELVDWARKENRKIKPLCPFIIAQMEKNSEYHDMLA"
     gene            9700..11034
                     /gene="mgtE"
                     /locus_tag="PKK75_03945"
     CDS             9700..11034
                     /gene="mgtE"
                     /locus_tag="PKK75_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNZ82777.1"
                     /translation="MKDERILELIEQKNYIELKKELSDMNEVDISEILEELEQNTSLL
                     VFRILPKDVAVEVFAHFSAEKQEEIISAVSDKELKFILGELFFDDMIDLIEEMPANIV
                     NKILTNSTHEERVLINQFLKYPSHSAGSLMTIEYVELKKNMTIQEALAHIKETGLSKE
                     TIYTCYVTDNKKKLEGIVSLRNLVISDSDMKVGDLLHEEIIYVETHDDQGTVANLFTK
                     YGFMAMPVVDKEHRLTGIITFDDIMEVIEDENTEDMQRMAAMTPLETTYLDSGVLELS
                     KQRIPWLLVLMLGASVTGGIITKFENLLSTYAILMAFIPMIMNTGGSSGNQSSTLIIR
                     GLATEDVTLKDAGKVLWKELRISLLVSVILGLVIYGKNVLTGKAGFMVSLTISLTMGV
                     IVMLATIVGGMLPLAAKKLKLDPAIMAGPLITTIVDALALVVYFGIASTLLF"
     gene            11279..11659
                     /locus_tag="PKK75_03950"
     CDS             11279..11659
                     /locus_tag="PKK75_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82778.1"
                     /translation="MRIHVIDQVTGMDVDNATITIYVTDGARRDIPVMHLITILNPIR
                     IELPMANVLGTQIVGPEYDFSTYNLRVDAFGYFANILYNIRLFPDITTDFSIELVPVT
                     QPQVYPAIEERIDIPPHPRDIIIE"
     gene            complement(11685..12509)
                     /locus_tag="PKK75_03955"
     CDS             complement(11685..12509)
                     /locus_tag="PKK75_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82779.1"
                     /translation="MPKTNFTPSKHGFHFRNDFINKIISTSFGKMETRGRCGGMAFAS
                     LDYYFSGIPIPAHESKDFPNGKTPPDGNLLAEYIYERTINSLFTMHSFKFFDWSLQKD
                     NPKGIRKSVSYKSKMEEFPKLMKSIDKGEPAVIGVISARKASEITHNHQVVVYGYEFD
                     AEKNHMALYTYDSNYPGKEIVIESCCSDDNFRSSAGQEWRGFFLQDYHPKKPKYIDID
                     TKITTEESVNVYSIKNIGQFPSNTKYLDIPKEIKENTGIILPGEEIKFSYIENQNT"
     gene            12858..13295
                     /locus_tag="PKK75_03960"
     CDS             12858..13295
                     /locus_tag="PKK75_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ82780.1"
                     /translation="MSGTLFALLAGLLMSVQGVFNTRVMESSNMWAATSFVHISAFAA
                     SMTLWFFSGREALFSVFEVSEKFYLLGGVIGAFITYTVIKGISDLGPANATMLILLAQ
                     LAVSYLIEVLGLFGTESTSFEWMKLIGVALMVSGIMVFCRSGA"
     gene            complement(13303..15093)
                     /gene="glmS"
                     /locus_tag="PKK75_03965"
     CDS             complement(13303..15093)
                     /gene="glmS"
                     /locus_tag="PKK75_03965"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNZ82781.1"
                     /translation="MCGIVGYIGFEDVKDIILGGLEKLEYRGYDSAGIAVLHNNEVYV
                     CKEKGRISDLRKNIDKEITGTVGIGHTRWATHGIPNKINSHPHQSSSKRFTLVHNGII
                     ENEEELKEKYLKGIELQSDTDTEVIVQLLEKFVNDDEETELAIRHTMSELKGSYALAI
                     IDKKDMNTLYAAKNKTPILAGKGIGFNMVGSDAMAMINHTNLFYEIEDKEFLKITKDN
                     IEIYTIYGNKVNRQPYKADIDASDTEKGTYPHYMMKEIEEQPFVIRKIMSEYSDKCGN
                     MSISPEILQQIRYCDKINIIACGTSYHAGLLGKQFFEKISHKPAEVILASEFNYNMPL
                     LTEKPLFMFVSQSGETADCRAVLVKVKEMGYKTLTLTNVKGSTLSREADQTLLLHAGP
                     EIAVASTKAYTAQITVLAILAALVENKIGMDVFKELSKIAYIMETLCNDRDKYKILAK
                     EFLLNSRNCFYIGRSMDYFLCLEAALKLKEISYIQTEGFPAGELKHGTIALIEKDTPV
                     IAVITQENINLNTRSNIKEVKARGAKTMVISMRKISQEKDHIVIDDANELLSPLAAII
                     PVQYLAYYAALLKGCDIDKPRNLAKSVTVE"
     gene            15718..15951
                     /locus_tag="PKK75_03970"
     CDS             15718..15951
                     /locus_tag="PKK75_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HNZ82782.1"
                     /translation="MASSNSGSGSNNIVVPEAKQALNQMKTEIANELGLTNYEQTDKG
                     NLTARQNGYVGGYMTKRLVEMAQRQMSGGGQFK"
     gene            complement(16043..16816)
                     /locus_tag="PKK75_03975"
     CDS             complement(16043..16816)
                     /locus_tag="PKK75_03975"
                     /EC_number="4.2.1.150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain-enoyl-CoA hydratase"
                     /protein_id="HNZ82783.1"
                     /translation="MEFKNLIVEKNERICTIKINNPQTLNALNGEVLNELDLAFDLVS
                     KDEEVAAVILTGEGKAFVAGADIAYMRGLNVEEGKKFAENGSRIFRKIEMLDKVVIAA
                     VNGYALGGGCELAMACDIRIASEKAKFGQPEVGLGICPGFSGTQRLPRLVGLGRAKEL
                     ILTGSHIDAQEAYRIGLANKVVNKDSLMEETYALAKKIVSNSRTAVKYSKEAIHRGNE
                     ADIETGIAIEANLFGLCFAHEDQKEGMTAFIEKRLANFK"
BASE COUNT         4897 a         3142 c         2736 g         6045 t
ORIGIN      
        1 aatatacctt gatattgttt cattttgtac agaaaccatg cggacaaatt cattttgaga
       61 aatcatattt ttaacttgta ttgggtattt tctataccaa atacatctgt gaatagttct
      121 atgaacttgt cttctgcacc gctggcaagg tgaaaggtat ttattgacat gactgttcct
      181 ctttaattat atacttttat atatataaat ctaaattgaa taataatata atttataaat
      241 tacttaccct tacttaaaaa catacctaca tttaggataa ttgctacatc caacaaactc
      301 tccatatttc ccatttcttt ctacaagcct tcctccacat cgaggacata tcccagcttc
      361 aaccttatat ttaaaattat ttattctttc aacgtgttcc attcttgcta cggcatcttc
      421 attatttgca ttaataataa tttgatgaat tctttctaaa tctacttgcg atatattttc
      481 gtcattatat aatcttattg tattaagcaa acctaaactg taaacaacat gagatgaaac
      541 atcaagcttt tttaaattag cttttaatgt aaatactaca atgggaatat atctaatcga
      601 agaataatct gataatatat cttgaatcgc tttaacatgt ccattatttt gaataattgg
      661 attgtaaaat tgattttttg ttttataaat aacctgtatc cagttcctgc tatgttcact
      721 accaaaaatc caaccattat agtttttagt ttcaatgaca aaaataccat atattgatat
      781 aataacatga tcaatttgag atgttcttcc atctgatgtt ggtattaata catcattaac
      841 aactatgtac ttttctttat ctaatagttt taataaaaat ctgatgaaaa tttcaccaaa
      901 gaaccctttt actgaaggta aacttagaat aaaagtaatt attacaatta taattaaata
      961 ttccataaaa cctccttacc ccaccatcac attcccactt gtcttcttct taataaactc
     1021 aggcatctta ttatccaact tccacactat actcattggt ttgctgcctt cataacttac
     1081 aaacttagct gtccctagac aataatacgg ttctgttata ccatttacac tttttctttc
     1141 ccttaagaat aatacaacct tactgcccaa tttctcatga ttgatatacc tttgtccagt
     1201 tggtgatatg tctgaagtta cgctttggga ctgccagtgg aagcattcat catctatggc
     1261 ataatcatca tacattgtgg tttcagagta atctttttct gatttattta atgttacaaa
     1321 gaatatatct gttttctttt catctaaata cttaactcct tgccaaaatg gtgccttata
     1381 accaagttta tattcttcta acgcagccaa tacttctgcc atggaatatc tactatgaag
     1441 ctttaatgga catggataac ctaaatttat atctttttct ttaaagtcga tgttttctaa
     1501 attgtattcc aagatagaaa taatttcatt taataagtga gggcatccct ttaatttatc
     1561 tatgcaatcc tttattttat caaatccaga tttttctaat gtatcttgcc ataattcata
     1621 gtagaacata tttagcacta attcctcata atcatttaag gatttataat caaatttgtt
     1681 agtcattaaa atctttaata taaactcaat atattctttt gaattaatat gtaagagtct
     1741 tgagatccca tctgattctt ttttatattg attataatca aagtctttaa ttactcctgc
     1801 ttctgcaaga agttgagtaa aactatagct tttataaata tcagttagct ctacattact
     1861 agacttgcaa aattcaacta catcatatgt gcttttgtta cttgcatatt ctatcagctt
     1921 tctttttata ttggtcttgt tatttataac attctttata ctttctaata tgtaatcctt
     1981 cgcaattctt tctaactgaa taaagcaacc ctttggtaaa tttggaaact catttcctat
     2041 ttccttttct accgtaaagt ttgtgttacc gattagtgcc ctaaactttt gctcataaat
     2101 gttatagctt ttatttgctt gtcctacata atctaatacc gtcaggcaat ccttaccatc
     2161 ataaagtctt aatccccttc ctagctgctg taaaaataca gtaatacttt cggtaggccg
     2221 tagaaataat accgtatcaa ttgctggtat atcaacgcct tcattatata aatctacaac
     2281 aaatataaaa ttaacttcct ttttatttaa cttacttttt gcactatctc tttcttcatt
     2341 gcttgaatct gaagtcaaat aaattgaagg aatacctacc ttgttgaaat acttgcacat
     2401 aaattcagca tgtagtttac ttacacaaaa gcctaatgct cttacatctt taatactctt
     2461 agtgtatttt tttatactat caataattaa attagctctt tgattgtttt tcgtataaac
     2521 cttttcaagc tcacttacaa tatatccgcc acttttccat tcaatatctt ttaatgatac
     2581 ttcatcggta acggcaaaat aattaaatgg acttagtatt tttctattta tagcttcacc
     2641 caatctaatt tcagctgcta ttttattatc gaaatattta gtaacgtcct tgccatccat
     2701 acgttcaggt gtggctgtca ttcctaataa tattttaggt ttaacatatt ctaataactt
     2761 tctatagctt ttagctgcgg agtggtgaaa ttcatctatt atgacatatt cgtaataatc
     2821 ttcttttata ttttcagtga attctttgga attaaatgtt tgaattgaaa tgaaaagatg
     2881 ttctatttga gaaggttcat atcttccgac ccataggtct ccaaagttcc tgtctcttaa
     2941 tatcatccta aatgcatcta aactttgttt taaaatttct tctctatgag caacaaaaag
     3001 aagcttgttt tttgagccag gattatcttc tacgaacctt ttgtaatcaa aacctgaaat
     3061 gattgtcttc ccagttcctg ttgctgccac taacaaattt ttatatctat tatgtacttc
     3121 acgttctgcc tggagtttat ctaagatttc tttttgataa gaatatggtg tgatgtcgaa
     3181 ataaaattgt gagccattac tgtctgtgtt actttctacc tttaaagcgt ttattaaagt
     3241 ttttgtatca ttttcgttat ctttattaaa tgaataaaac tcattatcat gccaataaga
     3301 ttcaaatgtt gcattaaatt tattaagcat ttctttggaa gtatattctg aaatttttaa
     3361 attccattcc aaaccagatg ttaatgctgc attcgaaagg tttgatgaac ctataaaggc
     3421 agtagaaaaa ccggtattcc tataaaacat ataagacttg gaatgaagtc tggttctttt
     3481 agtatcataa gatattttaa tttcagtgtt tggtagtttg gacaattcta atattgcctt
     3541 ataatctgtg gcacccatgt aggatgtagt tataatgcgc aattcatgtt cctttgtaaa
     3601 atcttttaat gcttcatata tttgaactaa cccgctccat ttaatgaaag aaaccagcat
     3661 atctattctt tcagctgtat ttatttcttt aactaattca cttcctaatg ttggttcgtt
     3721 tttagcacct gtaaaaagtg cattcatagc aatagaggta acaggtctta ttttatgatt
     3781 tttttctaat ggtgatttat taatgctgtt taaaacagca agcaataatt ctgcattctc
     3841 actaataata aactcattaa aatcatgcaa ttctaattca tctattaaaa gatttattat
     3901 tttattgcat atatcaattt gtttgcttaa tttttcttta ccggaaattc tgtttagaga
     3961 tttgttaatt actatagaca tgtattttga taagattgaa ctgctttctt cttcgtctat
     4021 ttgtttttta tcgatataat attctttttg atgcctaagt ttatgtattt cttcatctat
     4081 aaggttgttg ataacgctct catagaaacc attattaagt gacattgctc cccctctgcc
     4141 tataatatgt ttctcattat tcaaactttt ataaatttaa tacattgaat taattatagc
     4201 ataatgtgac aattaaatcc atacgaagaa ttaatttatt taataaaaat gatataaaaa
     4261 gtataaaaaa tcccgccata ataaagctat tatggtcggg attttatctt ttataaattt
     4321 atattagaat atggcattta aaattaacag ttcaacaaat ccaacagccc aaattattgt
     4381 tgttgtaaat gaccagactc tcatttgttc ttttgcatct tttatgccca gcatacggtt
     4441 aacaacccag tagtagctgt cgttaaagta tgaataacac aatgaacctg tacaggctgc
     4501 caatgccgca aacaccggat taacattcaa tgttgctata attggtgctg ttatggatgc
     4561 agatgtaatc atggcaaccg ttccgcttcc ctgaacaagt ctgattaaag tagctattac
     4621 aaacggcagt aatatgggag gaagtgctgt ttgggaaatg gcgtttgcta tatagtctcc
     4681 cgctcctgaa tcgcgtagaa ccataccgag tgacccgcca ccgccggtaa caagtatgat
     4741 gattcctgca gatttaattc ctctttccat taaatcaata gtttcatttc tggtatattt
     4801 tccggataag ccatatattg caactattaa gccaattccg acagcaataa ccggtgttcc
     4861 caaaaatact aatgcgttaa caacagtgcc tgaagctttc atagctgtta aaactgtgtt
     4921 tattaatatt aatattatag gaattacaat cggtgcaaat gacataaatg ttgaaggaag
     4981 attttcgttt tcttcattta atattgaaga ataatccggc tcctggtatg caggtcttat
     5041 ccagtcctca ccatcttctg taggaagctg gtagattttt ttgcctaaat attttgcata
     5101 taaaaccgct gctaaagtca taggtattgc caatactata ccccataaaa tcaatgaacc
     5161 tacgtttacg ttaaataatc ctgcaacacc aaccggtccc ggagtaggag gaaccaggga
     5221 gtgagtgata acaagtcctg ccgccaatga aactccaagg gttataactg attttttcgt
     5281 ttttcttgaa atagcctttg ccaaaggtga aaggataaca aatccggaat cacaaaatat
     5341 tggaatagat acaaggaaac ctgttattgc aagagctatt tcttctttgc ctttaccaag
     5401 cttcttaata aatgtttgtg ccattctttc tgctgcacct gatgcttcga atatttctcc
     5461 catcataaca ccaaagccaa ttatgatacc tatgcttccc agagtgtttc caaatccctt
     5521 ggaaattgat gataatacat catttgaagg cattccgcca actaagcctg ttacagctgc
     5581 tgcaataatc aaagccaaaa atgcatgaat ttttgttttc aaaattaaaa caattaaaag
     5641 ggcgatacca actaccaagc caaaaatcat ttggcttcca actacttgtt ccataatttc
     5701 ctccttgtta atttataatt ttatttattg aaattaggag catacaatgc cgctaatttt
     5761 attgcttcca ccatgcttac ttctcctgct ttgcctgtgc cggctatatc aaaggctgtt
     5821 ccatggtcta cagatgttct taagaaaggc atgttgttag ttatagaaat tgttctgtca
     5881 aagtcaacca ttttagttgc aatgtgtcct tgatcatgat aaagactcaa tactgcatca
     5941 tatctgccct tcaatccaaa ataaaagact gagtcggcag gaaccggtcc aacaacatct
     6001 ataccttctt cttttgcctt ttttacagca ggttcgattt catcaacctc ttcatatccg
     6061 aacaacccgt gctcgccgct gtgaggattt aagcctgcca ccgcaatttt aggatttttt
     6121 attcccaaaa ctctcaaagc ttcaatgcat cttgttataa actcataaac actgtccttg
     6181 gtcacaaggt cgcacgcttt tcttaaggat acgtgacgtg tgaggaaaaa tacccttagg
     6241 ttatggacct gaaacatagt caaaggattg tatgaatttg ttaaatcttc aagtatttca
     6301 gtatgtccaa tataaggaac ctccgcagct tttaaggatt ccttgtttat gggggttgta
     6361 gctatagcgt ccactttttt ttcaagagct aagttagcaa ctgttttaat atattcaaaa
     6421 gcagcttttc cacccatagc ttgcaccttg ccgattttca gccggtttac atccacattg
     6481 tttaaatcaa ttaaatcaat tgttccgtat tcatattttc cgtttgcggg ctctttaaca
     6541 gaattaatat ttaatgaaat atcacaaaac ttcattgctt gttccaatat gcttttgtca
     6601 ccgacaacca agggactgca aacttcataa atttcttttt cgtttaaagc ttttaccact
     6661 atttcaggcc ctatgcctgc agggtctcct aatgggatgc cgattattgg tttactcatt
     6721 attattctcc ttcttttttt gaattttttg caccatattc atttgataat ttagtcaata
     6781 aataattgac gcatttaatt aatgcttttt catctcccac aaggccgccc tttgtaataa
     6841 taggggtgtt gtggtatttt ccttttataa gtcttccgta aactgcaagt ggcagaacct
     6901 catccttaac ttcaatgcct gcacattcga gtgccttaca tacggcaacc gtaacgtctc
     6961 cgccggaggt ataaaggcct cctatttgat cttcggcttt tttaagcaga tttgttgtga
     7021 tttctgcaag cccgtttgtg atttttagtg aaacttcttc ttcatgaata ttaaggtcat
     7081 tggcaatttc agtaagattc aacacctggt tttcattcct ggtggtaata actcctataa
     7141 cctcgaaatc ctccatatta tccatgagtt tattaataat tctctccatt tcatgctctt
     7201 ttgtgtcttc ataaataagc ttcaatgcat ctgcttctac caaataagga gtgaattcca
     7261 atcttaactc atctatttgt tttcttgtaa ggttagatac gcttccaatt gtcagcatca
     7321 ccttttttcc gggactaatg gctggaactt cgagatattc cctggctaca gcagcggtaa
     7381 acggaccggg gtcaactgag atgatattaa gctttgaatt tttaactgcc ttggctattc
     7441 tgtcaatatc ttcatcagtt gttgcatcaa caacaattat tctgcatcct ctttttattt
     7501 ctgacagcag ggactgctct atggcaaatt caccctttaa aactttatcc agctctacaa
     7561 aacccacatt tttatcggtt tgttcttcca aaagggatac tacccgggag gtgtaaaccg
     7621 gtgtcttagg atctttggca acatctgttt tctccagggg gacctgatta accatcaaaa
     7681 atcctccgat gcaaaccctg cctgagctgg gaaatgcagg aacgactaag gcatatgtat
     7741 cctcttgcag gttgtctaat agtccggaaa tttcagagcc gatattacct cttaaagtac
     7801 tgtctattct tttggaaaat aatgcaacat catcatcctt aaagaattct gcaattgttt
     7861 taacccgttc ataagcttca tcactgtgta tcgctcttga atcagtgctt atggaaataa
     7921 cgtccaaata tttgctttct tccttgtcat acttgtctaa atttaaaaat gtagcagatt
     7981 taaaaccttg tcttgcaaga agtacgcctg tcgcattagc acctgtcaaa tcatcagcaa
     8041 taataattac ttttgacatt aggacctcct aaactaattt tattttaatt tttttacttt
     8101 taattttatc ggcagttagt tcatccaaat tgctgtcagt tattattcca tggaagtcgt
     8161 ttaaattaca aacctttatt aagctttttt tcccgaactt gctgctgtcg gcaaccagga
     8221 tacttttatc cgagcatttg atcatttgcc tttttacatc agctttttcc atggaaggag
     8281 ttgtaatgcc atgttgtatg tcgacggaac tgcttcctat aaatgaaata tcagcattga
     8341 tattttttaa aaagtccact gtgcttgaac cgtagcagga tccggttgag ttttgaactt
     8401 tgcctccagg aaataaaatt tcaaaatccg ttgatgcact tacaaaggtt gcaatgttta
     8461 aatcagttgt tataatttta agatttttat aatttaccag caatcttgca atttccatat
     8521 tggttgttcc tgcatccagt ataacagtat caccgttgcg tattaaagat gcagcataat
     8581 cagctatttt tttctttttt tcaatatttg cgagactctt gttttcatat ggaacttcat
     8641 caattaatct ggatttcaat acagcgccgc caaatgtgcg ggcagcaata tttttatcct
     8701 ctaaaaactg taaatccctt cgaattgtca ttggggcaac attaaataat tttgccagtt
     8761 cttcaactgt tactgcctca tgttcttcaa gatattgaat aatcatttgc cttctttcaa
     8821 tttgcagcat gttacctccc aaatgaatgt ttgactttgt ttgtttgtgt tattttatgt
     8881 tcattatagc attgatatta ttattgtcaa gagaaatttt taaaaatttt ttaatttcgc
     8941 acgctttggc tatgggcaac ttaaacataa atttattatc agattattgt attttgctgc
     9001 aaaataataa acacagatga actatctgtg tttattaaaa cttaattata ttctttcatt
     9061 gacatttgaa ccattccatt ttcatattaa cctcccttaa gctaacatat catggtattc
     9121 agaatttttc tccatttgag ctataataaa ggggcagagg ggttttatct ttctgttttc
     9181 ttttcttgcc caatccacta attccttcaa tatgagtttt cctgctcctt ttccgcctaa
     9241 ttcctcagat acttttgtac tttcggcaac gataattcct tcttcattga tagaaaatat
     9301 tatttctgcc aggggagctt cctctattcc tatgtaaaat ttattctttc cttttttaac
     9361 ttccattatt tttcctccaa atatttgttt aaaacattat aacataatca tcaccaaagg
     9421 gcaaaaaaga taacagcacc cctgtcccct tgtcatataa aataaattct tttttcaaat
     9481 tttctttaag atataatttt agtgtgatat aatcatatac aggaatattg aatataatat
     9541 tctaaatact ttttaaggag tggtgagtat ggactctgac cctttaagta catgcaaagc
     9601 ggtcaaatat aattttacat tttcagggaa gacgtattcg ccgctgcggt ttctctgatt
     9661 gattaatgga catttgtact ataatttaag gagggaccaa tgaaagatga aagaatactg
     9721 gagcttattg aacaaaagaa ttatattgaa ttgaaaaaag aactttcaga tatgaatgaa
     9781 gtagatattt cagaaatatt agaagagctg gaacaaaata catctttgct ggttttcaga
     9841 atacttccaa aggatgttgc cgttgaggtt tttgctcatt tttcggcaga aaagcaggaa
     9901 gaaataatct ctgcggtgtc ggacaaggaa ctgaagttca ttttgggcga gttgtttttc
     9961 gatgacatga ttgacttgat tgaagaaatg cctgctaata ttgttaataa aattttaacc
    10021 aattcaaccc acgaagagag ggtattaata aaccaattct tgaaataccc ttctcattcg
    10081 gccggaagcc tgatgactat cgaatatgtg gagcttaaaa aaaacatgac gatacaggaa
    10141 gcattagctc acatcaagga gaccggatta agcaaggaaa caatttatac atgttatgtc
    10201 acagataata aaaagaagct ggaaggaatt gtatcgctta gaaacctggt gatttcagat
    10261 tctgatatga aagtagggga cctgctgcac gaagaaataa tctatgtgga aacccatgat
    10321 gatcagggaa cagttgcaaa tctttttacg aaatacggct ttatggcaat gcctgttgtt
    10381 gacaaggaac accggctcac cggaattatt acatttgatg acatcatgga agtaatagag
    10441 gatgaaaaca ccgaggacat gcagagaatg gctgccatga cgcctttgga aactacctat
    10501 ttggattcag gggtactgga attatccaag caaaggattc cctggctttt ggtattgatg
    10561 cttggggcgt ccgtaaccgg cggcattatt accaaatttg agaacctcct gagcacctat
    10621 gctattctta tggcgtttat tccaatgatt atgaatacgg ggggaagttc cggaaaccag
    10681 tcttcaacct tgataatccg cggattggct acagaggatg taactttaaa ggacgcaggg
    10741 aaggttttgt ggaaggaact gagaataagc ttattggtat cagttatttt gggtttggtg
    10801 atttatggga aaaatgtgct taccggcaag gcaggattta tggtttcttt aacaatttcc
    10861 cttactatgg gagttattgt tatgttggca acaatagtcg gagggatgct tcccctggca
    10921 gctaaaaagc taaagctgga ccctgcaata atggctggcc cgctgattac cacaattgtg
    10981 gatgcattgg cactggtagt ttatttcgga attgcttcaa cactgctgtt ttgagcaatg
    11041 tgacggcacc cccgtcccca tgacattagt cacaccattg catataatga tcatataata
    11101 ttatctttat taattaggga aaggaaggaa atatattatg ataaatatgc ctggaggccg
    11161 atttactgat tattatccac ggtcttttaa tgcaggccgt tacaattata ggttataccc
    11221 tggtcaaaac ttatatcaaa gccaaaataa tatatcacaa aataatgccg gcggtttcat
    11281 gagaatacat gtaatagacc aggtaaccgg aatggatgta gataatgcaa ccataacaat
    11341 ctatgtaact gacggggcga gaagagatat cccggttatg catttaataa caatattgaa
    11401 tcccataagg atagaactgc caatggcaaa tgttctggga actcaaatcg ttggacctga
    11461 atatgatttt tcaacttata atttaagggt ggatgctttc ggatattttg caaatatatt
    11521 gtataacatt cgtttgttcc cggatataac aactgatttt tcaatagaat tggttcctgt
    11581 aacacagcct caggtatatc ctgccataga ggaaaggata gacatcccgc cccatcctag
    11641 agatataata atagagtaaa taaatttaaa cacttaagga taacttaagt gttttggttt
    11701 tctatatatg aaaatttaat ttcctcaccg ggaagaatta tgccggtgtt ttcctttatc
    11761 tcctttggaa tgtcaagata ttttgtattt gatggaaatt gtcctatatt ttttatggaa
    11821 taaacattaa ctgattcctc tgttgttatt ttagtatcta tatcaatata tttgggtttt
    11881 ttcggatggt agtcctgcag gaagaatcct ctccattcct ggcctgcaga tgaccggaaa
    11941 ttatcgtctg agcagcagga ttcaatgaca atctccttgc cgggatagtt gctgtcatag
    12001 gtgtaaagtg ccatatggtt tttttcggca tcaaattcgt agccgtatac aactacctgg
    12061 tggttatggg ttatttcgga tgccttcctt gcactgatca cccctattac tgccggttct
    12121 cccttgtcga ttgatttcat aagcttgggg aactcttcca ttttgctttt ataggaaaca
    12181 ctttttctta tgcctttcgg attgtccttt tgcagggacc agtcgaagaa cttaaatgag
    12241 tgcatggtaa ataaagagtt gattgttctt tcatatatat attctgccag cagattgccg
    12301 tccggcgggg tcttgccgtt tggaaagtcc ttcgattcat gtgccggtat tggaatgccc
    12361 gaaaaatagt aatccaatga agcaaatgcc attcctccgc atctgcctct ggtttccatt
    12421 ttgccgaagc ttgtgctgat tattttattg atgaaatcat ttctaaagtg aaatccgtgt
    12481 tttgaaggtg taaagtttgt ttttggcata aactcctcct aaaacgctgg tatataattc
    12541 tcgtttctta aaaatggatg taagaaatcc ctgcatctat gcagtatgtg ttgcctaaaa
    12601 gatgttttgc cttcatttct gtctctaggt cttctctagt aagaataata ttataatctg
    12661 tttaaattgt caattattat taggttctga tactttaccc ctgataattt tttattggta
    12721 attttacaaa cacttttatc ggcgacttaa attttttatt catattgttt ttaattaaac
    12781 cgggtatttt tgggatacta taaaaaaagc aaaaaaacag gataacaaat acagatcatt
    12841 attaggagag gattaatata tcaggaactt tatttgcgct gttggcagga ctgttaatga
    12901 gcgtgcaggg agtttttaat acccgcgtaa tggaatcatc aaatatgtgg gctgcaacaa
    12961 gctttgttca tatatcagct tttgcggcaa gcatgacttt atggtttttt agcggacggg
    13021 aagctttgtt ttcggtgttt gaagttagtg agaaatttta tttgcttggc ggtgtcatcg
    13081 gtgctttcat tacatatacc gtcattaaag gaatatctga tttagggcct gccaatgcta
    13141 caatgctgat tctgctggct cagcttgctg tttcatattt aattgaagtg ttgggcttgt
    13201 tcggcactga aagtacaagc tttgaatgga tgaaactcat cggagttgcc ctgatggttt
    13261 ccggcataat ggtattttgc aggagcggtg cttgaccgcc acctattcca cagtaaccga
    13321 ttttgccaaa tttcttggct tgtcaatatc acatcctttt aacaatgcgg cataataggc
    13381 caagtattga acagggatta tagctgctaa aggcgatagc agttcatttg cgtcatctat
    13441 tactatgtgg tctttttcct ggctgatttt acgcatggat ataaccattg ttttagctcc
    13501 tcttgctttg acttccttaa tgttgcttct tgtgttgaga ttgatatttt cctgcgtaat
    13561 tactgcaatt acaggagtgt ccttttcaat taatgcaatt gtcccgtgct ttaattcacc
    13621 tgccggaaat ccttctgttt gaatatatga aatctctttc agcttaagag ctgcttcaag
    13681 gcataaaaag tagtccatgc ttcttcctat atagaaacaa ttcctgctgt ttaatagaaa
    13741 ttcttttgct aatattttgt atttgtctct gtcattgcaa agagtctcca tgatgtatgc
    13801 gattttggat aattccttaa aaacatccat gcctattttg ttttcaacca gggctgcaag
    13861 tatagccaat actgtaattt gagctgtgta cgccttggtg gatgcaacag ctatttcagg
    13921 tcctgcatgt aacaaaagcg tttggtctgc ttcccttgac agcgtagagc ctttcacatt
    13981 ggtcaatgta agagtcttat agcccatttc ctttactttg accaatacag ctctgcagtc
    14041 tgccgtttcc ccgctttgcg aaacaaacat gaacaagggt ttttcagtta acagcggcat
    14101 gttgtagttg aattcactgg caagaattac ttctgccggt ttatgggata ttttttcaaa
    14161 gaattgcttg ccaagaagtc ctgcatgata gctggtgccg catgcaataa tatttatttt
    14221 gtcgcaatat cttatttgtt gtaaaatttc cggggaaatg ctcatattac cgcatttatc
    14281 ggaatattct gacatgattt ttcttattac aaagggctgt tcctcgattt ccttcatcat
    14341 ataatgagga tatgttccct tttccgtgtc tgaagcgtca atatccgctt tgtacggctg
    14401 cctgtttact ttgttgccgt agatagtata tatttctata ttgtcctttg ttatttttaa
    14461 aaattctttg tcctcaattt cataaaatag gtttgtgtgg tttatcattg ccatggcatc
    14521 gcttccaacc atgttgaagc cgatgccttt tccggctaaa atgggtgttt tgttttttgc
    14581 agcatagagt gtattcatat cttttttatc aattatggca agagcgtagg accccttcaa
    14641 ttcactcatg gtatgcctta tggcaagttc agtttcttca tcgtcattaa caaatttctc
    14701 caatagctgc acaattacct ctgtatcggt gtcgctttga agctcaatcc cttttaagta
    14761 tttttctttt aattcttctt cattttcaat aattccgtta tgtaccaggg taaaacgctt
    14821 tgagctgctt tgatggggat gggagtttat tttattggga attccatggg ttgcccacct
    14881 tgtatggccg attccgacag tgccggttat ttctttatct atgttttttc ttaagtcaga
    14941 aatccttcct ttttctttgc atacatatac ttcattgttg tgtaaaactg caatgcctgc
    15001 cgagtcatat cctctgtatt caagcttttc aaggcctccc aaaattatgt ctttaacatc
    15061 ttcaaatcct atatatccta ctattccgca catttatttt ctccattctt ttaatttttt
    15121 tgaattacaa aaacatgtct gtaattctgt aattttatta tgggatttgg gaaacctaga
    15181 ttattatttc aataagattt gaagctgtcg attttatttt gtcattacag tcgcctgtaa
    15241 tatttgtaaa aaccgtatcg gtgcagctgc accgcagagc atccgccgaa ttttcgataa
    15301 ctctgtcctc gtcaactttt aataaagttc cggcgctata atattccttt tgtttccccg
    15361 cttaaacata ttgtttattt aaacaatatg ttgcattctc ttttatataa tagcataaaa
    15421 tcatttttaa tgtatatatt tttttccaaa tattttttgg tttccaattc tccaaatgaa
    15481 ttacttttat gcattttatt gccatgtatt ataatttgtt tgcttgtcaa taataagttt
    15541 agaaagtcat aaaaatgatt ttcaaattaa aatttaatca atgtaaaaat ttaacctgtt
    15601 acatagaaca ggttcaaagg gcccaaagtg atttaccaat ttagcataat attacatgat
    15661 taaattaaag ctttttgaca ataataagaa tacatcttat gagaaaggag aataagaatg
    15721 gcatcaagca acagcggaag cggaagcaac aacatagtag ttcctgaagc aaaacaagct
    15781 cttaaccaga tgaaaactga gatagctaat gaattaggtt taacaaatta tgagcagaca
    15841 gacaagggta accttactgc aagacaaaat ggttatgtag gcggatacat gactaagaga
    15901 ttggttgaaa tggctcagag acaaatgagc ggcggcggac agtttaaata aaaatgaagt
    15961 atagtaagtt tggtggatag aaactatcca ccaaattttt aattttttat gaccttttga
    16021 taagtctttt tctcttttga ttttatttga aatttgccag ccgtttttct ataaaagcgg
    16081 tcattccttc tttttggtct tcatgggcaa agcataaacc gaataaattt gcctctattg
    16141 caatgcctgt ctcaatatct gcctcatttc ctctgtgaat tgcttccttt gaatatttta
    16201 ctgcagtcct ggagtttgaa acgatttttt tagccagggc atatgtttct tccatcagac
    16261 tgtctttgtt tactacttta tttgcaagtc ctatccggta agcctcctga gcatctatat
    16321 gggagcccgt caatattaat tcctttgccc tgccaagccc caccagtctc gggagtcttt
    16381 gggttcctga aaatcccggg caaattccaa gacctacttc cggctggccg aattttgctt
    16441 tttcagatgc aattctgata tcacaggcca tggcaagctc gcaccctcct ccgagagcat
    16501 agccgtttac agcagcaatt acaaccttat caagcatttc aatctttctg aaaattcttg
    16561 acccgttctc tgcaaatttc tttccttctt ctacgttgag tcccctcatg tatgcaatgt
    16621 ccgcaccggc tacgaaagcc tttccttccc ctgttagtat cactgctgcc acttcttcat
    16681 ccttggaaac aaggtcgaaa gccaggtcaa gctcgtttaa aacttcaccg ttcaaagcgt
    16741 tcaatgtctg aggattattg atttttatag tgcatattct ttcatttttt tcgacaatca
    16801 aatttttaaa ttccataact
//
LOCUS       DAOPUT010000027        16622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3527_length_16622_cov_20.063439, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000027 DAOPUT010000000
VERSION     DAOPUT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16622
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3527_length_16622_cov_20.063439"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKK75_03980"
     CDS             complement(<1..458)
                     /locus_tag="PKK75_03980"
                     /inference="COORDINATES: protein motif:HMM:NF022378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2680 domain-containing protein"
                     /protein_id="HNZ82784.1"
                     /translation="MKKFNKTLIVSLVLLIVLAASITVYAFTFKTPAEIVGGLTGKST
                     EEVAELRSEGKTYGQIAYDDSEETWKQFRDEMLENKKAILEERVEEGILTQEEADKIL
                     ENIEARQEACLEAGGGYGMGNGFGMRNKLGNGDGFGMGNGFGNGNRGMGFG"
     gene            622..1311
                     /locus_tag="PKK75_03985"
     CDS             622..1311
                     /locus_tag="PKK75_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ82785.1"
                     /translation="MATMLIADDNRQITSILEEYAKKEGYAVKIAYNGRQALETFNQY
                     KPDIVLLDVMMPIMDGFEVCREIRKVSNVPVIMITARGEDFERIMGLDIGADDYIVKP
                     FSPAEVMARIRAVMRRIGADNVSSQLFTYDNLRINMEDYMVTIDDNNVSLTKKEIEIL
                     WTLAVNKNKVFTRDNLLNSLWGYDYYGDSRTVDSHIKRLRSKIDEFDHKNWEVRTIWG
                     VGYKFEVYDEK"
     gene            1301..2773
                     /locus_tag="PKK75_03990"
     CDS             1301..2773
                     /locus_tag="PKK75_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ82786.1"
                     /translation="MKNKISIKLIVYFSAALIVFSIIIGGIFMTLFKNNTLEIHKKDL
                     ERRALTIADTISQLMDGTSLDFSPGMGRHGGMMGMQGSLGLYIRSLDDIAMADAWIVD
                     EEMNLLITGNMARMQYSYADLPQDADIVVKDVFKGKTSFSEGFSRLLESPALTVGTPI
                     TSDGEIIGALLIHSPIEGMDDAISEGFRILLFSIVTALILSVFLSVALALTFTRPINR
                     MKTTAAFLAEGNYSRKTGIRQNDEIGDLAATMDILSQRLSDADEERNRVQKQRQDFIT
                     NISHELRTPVTVIRGSLEALSDGVVSEPDQVKEYHKQMLNESKSLERLVNDLLELSRL
                     QNTDFKIEKQDLNICDVLKDSARSASNLARQKNIKIEYSLDRDVFLINGDYGRLRQMF
                     LTVIDNAIKFSYEGTSIYITLKNNRITIKDEGTGISEGDLPYIFDRYYSANSGENKTG
                     TGLGLTIAKQIALRHNIEILAESRPGEGTSFVFVIPGDLE"
     gene            2977..3795
                     /locus_tag="PKK75_03995"
     CDS             2977..3795
                     /locus_tag="PKK75_03995"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNZ82787.1"
                     /translation="MYMEIIKAIILGIVEGITEWLPISSTGHMILVEEFIKFDASQEF
                     MEMFRVVIQLGAIMAVVLLYFHKLNPFAPSKTQKQKRDTVDIWFKVIIGVLPAAVLGF
                     LFDDWFDENFYNYQTVAVTLILYGILFIIIENKNKGKQAEIKSFKDLSYKTALIIGFF
                     QVLSLVPGTSRSGATILGAIILGTSRNIAAEYSFFLSIPVMFGASALKLVKFGFEFTG
                     IELAVLVTGMVVAFIVSVIAIRFLIRYIKNNDFKVFGWYRIILGILVLAYFMFT"
     gene            4001..4306
                     /locus_tag="PKK75_04000"
     CDS             4001..4306
                     /locus_tag="PKK75_04000"
                     /inference="COORDINATES: protein motif:HMM:NF041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CC/Se motif family (seleno)protein"
                     /protein_id="HNZ82788.1"
                     /translation="MNDENIIRITEKAKKHLNKKGKLQVSIEIPENRLSSESVLVPIP
                     EIIARKPKVPENYRLFTVDGIDVYVSNSVAMPKNEVVIDLDIFLGIKLLNLSGFKSS"
     gene            4316..5500
                     /locus_tag="PKK75_04005"
     CDS             4316..5500
                     /locus_tag="PKK75_04005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNZ82789.1"
                     /translation="MKRVVFLVDMNAFYISCEKTRRPDLEGKPAAVAGDPVNRTGIVL
                     TANYEARKYGVRTAMVLREAFRLCPDLITIPPDHSFYEMKSKEVMKILSDYTPLVQQN
                     SIDEAWLDMTGCEILFGKPLESAKSIMERIRTELDLWCSIGISENKFLAKMASEMKKP
                     LGITELWVKDIQEKMWPLSVSYMYGVGRQTAAKLNEMGIFTIKDLALANKDNVYKRLG
                     KTGAELVLLANGIDTSPVEQNKEDMKSIGRSTTLPCDIVDIEYAKSILMELSDDVGMT
                     ARKHGKKGHTVQITIKYSNFNTITRQTTINPTCNVKDIYAAGETLLENNWNKEPVRLL
                     GISLSGFDKNTQQISLFQVMDKSEEKQDDKIDNLEETIHKLRQKYGTEIIKPGIHVKK
                     EE"
     gene            5590..7272
                     /locus_tag="PKK75_04010"
     CDS             5590..7272
                     /locus_tag="PKK75_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82790.1"
                     /translation="MGKSNEKQRAWNIIWSVSKDYSLTPDITAYDERGKADLYWNYII
                     GAVYKYFDYRLLSEFFDTLRYDRDYIFYQKIMMLGLEGCIFEKDSKNRPVLTGLRSEY
                     GKKAYENRDEAELFDEIQAAYFSRVNGYTLKIRDNINSLLDDLNFDISLGTGQLVFKM
                     EQIIKKYFEPEYNDEFYIKKIKRNRLLHFFGRKHPSIRRSIKGGTLTLIGNDEGKGAK
                     GGKIFSGLLKLKEKSDNKEREFIKDYFGISTIGETNTKALEKVLCDEAHESCHLHFTR
                     GQFDDTDDAIFRQKQTSVQRDKNKKHYIKNYIRNTNNIAKLTQRIKNAIIVNYTYSMK
                     SESGKLATEKTWRTILNDSKIFIKIIKDEMPDISVDIMLDASASQLNRQETIAEEGYI
                     IAESLTRCNIPVRIYSFCNMRDYTVMNLFRDYNEINNNDRIFSYCAAGFNRDGMALRT
                     ALHMMGDSKYNHKILIVLSDVKPNDMLGVASEGFLPLQKEYSAETGVNDTANEVRKGW
                     KQGVSILCVFTGRDEDIPSVKKIYGQKFTRILTLDKFAEMVGLLIEKELINR"
     gene            7284..9005
                     /locus_tag="PKK75_04015"
     CDS             7284..9005
                     /locus_tag="PKK75_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82791.1"
                     /translation="MKKLLPLMKKYMKYAVLCPILMVLEVAADIAIPYLMSRIVDVGI
                     ANQDIKYVIQIGLIMIIAAFLGMTFGVMSSHYGAKAGFGFASEVRMKAYKKIQGFSFA
                     NLDKFTVASLITRLTNDCNTLGQVTMMSLRMAVRAPFMMIFALLMAMNINASLASVFM
                     VSIPVLVLAIIIILTKARPLFLKMQTRVDRVNAIIRENLTAIRVVKSFNRQSHEEKRF
                     KERNDSLMDTALKAITLIIFLMPVLNIIIYSTIIAVLWFGGRQVVAGSMGSGELISFI
                     TYITQILMSFMMMSMFFMQLLRASASVTRIVEILDTESEIKEVENPVKELTDGSVSFK
                     DVCFCYPGSTEKTLKNINFHINSGEVLGIIGSTGSSKSTLVQLIPRLYDTMEGSVVVG
                     GRDVKDYDIKSLRDHVAFVLQNNTLFSGSIRENMKWGNENAEDEEIIKALKLAQAWEF
                     VSAYKDGLDHRIEQGGDNFSGGQKQRLTIARALLKSPRIVILDDSTSAVDMATDAKIQ
                     RVFKNELSDVTTIIIAQRVSSIQHADRVLVMNEGEIESLGRHEDLLVSSPVYREIYES
                     QQKGVRP"
     gene            9002..10822
                     /locus_tag="PKK75_04020"
     CDS             9002..10822
                     /locus_tag="PKK75_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82792.1"
                     /translation="MKQKQLRPRDPKKTLLRLFSYFKYNLALFFGGIFFIIIGSLAEI
                     GINGMLSPVIDTLVGDHDRGLFIKYLLIMAAMAAVVSSAQYISNLSMAKLAQKTVHKI
                     REDMFSHMEKLPVSYFDKHSHGELMSTFTNDVDMLNQSLEQSVSQILITSVTVVGTFI
                     MMLVLSPVLTAVVAVMLAVMLYSVRFVGKRSAENFRMQQAALADMNGYIEEMMSGQKV
                     VKVFNYEDRAIDTFSKKNEELRNAGTLASTYGVMMMPLMGNLSFVMYALISMAGAYLV
                     MIDLLSIGNIASFLQYTRSISRPITMVSNQLNTLFAALAGAERIFDVLDEKFETDGGD
                     VTLLKDCEGRKNPCWMVPKTDGGNDEVPLKGFIAFKDVDFGYTTEKKVLNKINLYAKP
                     GQKIAFVGSTGAGKTTVTNLINRFYEINEGVILYDGIDIKRINKFDLRSTMSMVLQDV
                     HLFEGTVKENIRYGRLDATDDEVVEAARLANADYFIQHLPEDYDTVLTSDGQNLSQGE
                     RQLLSIARAAIADPIILILDEATSSVDTRTEKLIAEGMDKLMAGRTTFVIAHRLSTVR
                     DANAIMVLENGEIIERGDHDDLMAQKGRYYELNAGTIELT"
     gene            10911..11771
                     /locus_tag="PKK75_04025"
     CDS             10911..11771
                     /locus_tag="PKK75_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HNZ82793.1"
                     /translation="MNYGWIIYPKDTINNKFGNNAFEWMKESALRNDIRIDIIFSDDL
                     IILNNNKKTEFMYLGKTLSLPDFVIMRDYNYAVSMQLENLGIKVVNSTGSMFNSRNKA
                     VTAQLLVKNNVSTPDFLFTKEKNYELIHNYFNGRKFVMKTLEGSQGKGVYLIENEEDY
                     NSTYLEINGEYFCQEFIDFSCGMDLRVYVLGNKVLGCVKRISDSSFKSNYSLGGRVEK
                     FELTDSIKEISLSAAKAVGLDFCGIDLLFTKDSFTVCEVNGNAGFRTITKVSDIDIPM
                     ELFKWIKRSL"
     gene            11949..13139
                     /locus_tag="PKK75_04030"
     CDS             11949..13139
                     /locus_tag="PKK75_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ82794.1"
                     /translation="MIDIKDTLFNITEKHKEAAELLISLGFDNLKDDNLRKIFGSSVS
                     LENALKYKKINVDEFVNQLMERIKDNDTAKNKYKDTIKISGVLPCPVKVPLMETFEKW
                     LGEQDFDFNLNYDLKAASMGLGWIKDELKNSDKVPDLLISAGFDLFFDKNLLGKYKSE
                     NLFEDITGLQKYNQDFEEYNLEDPSGQYSMLGIVPAVFLVNKDELKGRKAPQSWEELM
                     SGEYENKVSLPISDFDLFNALLLNICKLYGEEGIRRLAGIFQKNMHPSEMVKSHMKRE
                     RPAITIMPYFFTWMVREGSPMELVWPKDGAIISPIFMLSKRETKDKTKVIADFFASRE
                     AGEILSHKGKMPSVNPQVDNQIPKENKYIWLGWDFIKENDISDLIKKCENIFNETLKG
                     DDGI"
     gene            13136..13843
                     /locus_tag="PKK75_04035"
     CDS             13136..13843
                     /locus_tag="PKK75_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ82795.1"
                     /translation="MNLVIISGPPSSGKTSVILKTIEAIKKRGINAGVVKFDCLYTDD
                     DILYERAGIQVKKGLSGSLCPDHFFASNIEEVVRWGKKEGLELLITESAGLCNRCSPY
                     IKNIKAVCVIDNLSGINTPRKIGPMLKTADIVVITKGDIVSQAEREVFASRVNSVNPQ
                     AVIMSVNGLTGQGAFELSTLIYDKNANIETVKGSELKFPMPSALCSYCLGETRIGEEY
                     QMGNVRKMNIGEENDKH"
     gene            13830..14834
                     /locus_tag="PKK75_04040"
     CDS             13830..14834
                     /locus_tag="PKK75_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82796.1"
                     /translation="MTSIRELEMKKIKDLEAQYPFVLSFFENNKLSVEGFKDSTFIEY
                     LNHFTQEEIEEWAINIPKIKSDLEAYVNQMLQFLGLEKETVDSITILAGRDKYGNREN
                     FNELTINKSEIVSIVGPTGSGKSRLLADIEWAANGDTPTERVVLINGEKPDYSMRFST
                     SNKLVAQLSQNMNFIMDLTVREFLELHAKSRMVENQEEVVLKIVEAANELAGEKFDPD
                     TPVTGLSGGQSRALMIADTAILSTSPIVLIDEIENAGIDRKRALNVLVNEEKIVLMAT
                     HDPSLALMADKRIVIKNGGIHKVIETGTKEKEVLKELEKMDKIINSMRTSLRNGDSIA
                     "
     gene            14885..15790
                     /locus_tag="PKK75_04045"
     CDS             14885..15790
                     /locus_tag="PKK75_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003198234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ82797.1"
                     /translation="MTKLKADLSLLMVTLGWGISYYLTDLSLEDMGPFTLNSNRFLIA
                     FAVASILAFPKLKNISKHTFKFSLILGVALVFVYLGATFGTKYTSLSNVGFLCGLTVI
                     FTPILSAVIYKKLPGKKQTLVILLSFVGIALLTLKDDFSINYDNLLGDLLCIMCAFAY
                     AVHLLITEKAVTFEDVNAFHLGVLQLGTTGVFTLILALIFETPHLPTEPRYWGPVLFL
                     SIFCTGMAFIVQCVAQQYTEASHVGIIFSLETVFAGIVAFVLAHEVLSFQSYVGAVLM
                     VASIFIMEIDFSKFRLCRKNQNEPE"
     gene            15935..16207
                     /locus_tag="PKK75_04050"
     CDS             15935..16207
                     /locus_tag="PKK75_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82798.1"
                     /translation="MPINDNVHEQMQNWELQQKQESQKELRDKYEYMNDKYQKMKNDY
                     TSFKSARRDKNEEIYNNSSDQTGKNPYMRYELNNTFKNKDDKKESK"
     gene            16340..>16622
                     /locus_tag="PKK75_04055"
     CDS             16340..>16622
                     /locus_tag="PKK75_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNZ82799.1"
                     /translation="MKTLEELSDELKKNNIRLTHQRLKVLEYLSNSKDHPTVEQIYNA
                     LKREVPSLSKTTIYNTLNYLSELNLIKVLAIEENEAHFDAVKESHGHFKC"
BASE COUNT         5742 a         2737 c         3518 g         4625 t
ORIGIN      
        1 ccgaatccca tgcctctgtt gccgtttccg aaaccgttgc ccattccaaa gccgtcgccg
       61 ttgcctaatt tatttctcat tccgaagcca ttgcccattc cgtaaccgcc gcctgcctca
      121 aggcatgctt cctgtcttgc ttcaatgttt tcaagtattt tgtctgcttc ttcctgagta
      181 agaattcctt cttcaactct ttcttccaaa atggcctttt tgttttcaag catttcatct
      241 ctgaattgtt tccaggtttc ctcgctgtca tcataggcaa tctgcccgta tgtttttcct
      301 tcagatctta actcagctac ttcttcagtg cttttgccgg ttaatcctcc aactatttct
      361 gcaggtgttt taaatgtaaa tgcataaacc gtgattgatg ccgccaatac tattaacagt
      421 accaagctga ctattaaagt cttattaaat tttttcattt ttgacctcct attttttatc
      481 tatgtttaat ttaaccaata tttgtgtcaa atttatgtct ttattataaa aaaaatatga
      541 aattattctt ggtaaaaaca caatcttgcc acaatttaac tttagtatga tataattaca
      601 tagagaatgt gaggtggaca tatggcaaca atgttaattg cagacgacaa caggcagata
      661 acatccatac tggaagaata tgcaaaaaaa gagggctatg cagtgaaaat tgcatataac
      721 ggcagacagg ctctagaaac atttaatcaa tacaagccgg atattgtcct gcttgatgtt
      781 atgatgccca taatggacgg atttgaagta tgccgtgaaa taagaaaggt ttctaatgtt
      841 cccgttatta tgattacagc caggggagag gattttgaga gaattatggg tcttgatatt
      901 ggtgctgatg attatattgt taaaccattt tctccagccg aagtaatggc aagaattagg
      961 gctgtcatga gaaggatagg ggctgataat gtaagcagcc aattattcac atatgataat
     1021 ttgcgaataa atatggaaga ttacatggtt acaattgatg ataacaatgt ttccttaaca
     1081 aaaaaggaaa tagagatcct gtggacactg gctgtgaata agaataaagt ttttaccaga
     1141 gacaatcttt taaacagttt gtggggatat gattattacg gggacagcag gacggtggat
     1201 tctcatataa aaagactcag gtcgaaaatc gatgaattcg accataaaaa ttgggaagtt
     1261 agaacaatat ggggagtagg ctacaaattt gaggtatacg atgaaaaata aaatttccat
     1321 aaagctgata gtatattttt ctgctgcatt aattgttttt tcaataataa taggcggaat
     1381 tttcatgacc ttgtttaaaa acaatactct tgaaattcat aaaaaggacc ttgaaaggcg
     1441 tgccttgaca attgcggata caatttctca gttaatggat ggtaccagtt tggactttag
     1501 tcccggtatg ggcagacacg gcggcatgat gggcatgcag gggagtctgg gtttatatat
     1561 acgcagttta gatgatattg caatggcaga tgcatggatt gttgatgagg agatgaatct
     1621 tctaattacc ggaaacatgg cccggatgca gtactcttat gccgacctgc cccaggatgc
     1681 ggatattgtt gttaaagatg tgtttaaagg caaaacaagt ttcagcgaag gcttcagcag
     1741 gcttttggaa tcacctgcct tgactgttgg cactccaatt acctcagacg gcgaaattat
     1801 aggagcgctt ttgattcatt caccgattga aggtatggat gatgcaatta gtgaaggctt
     1861 caggatttta ttgttcagca ttgttaccgc actcatttta tccgtatttt tatccgttgc
     1921 attggctcta acatttacaa gaccgattaa taggatgaaa accactgcgg cttttctcgc
     1981 cgagggaaat tacagcagga aaaccggtat cagacaaaat gacgaaatag gcgacctggc
     2041 agcaacaatg gacatattga gccaaagact aagtgatgca gacgaagaga gaaacagagt
     2101 ccaaaaacag cgccaggatt ttataacaaa tatatctcat gagttaagaa ctcccgtaac
     2161 tgttatcagg ggctctttag aagctctctc agacggagtt gtaagtgagc cggaccaggt
     2221 caaggaatac cataaacaga tgctgaatga aagcaagagc ctggaaaggc tggtaaatga
     2281 tcttttagag ctgtcaagac tgcaaaatac cgactttaaa attgaaaagc aagatcttaa
     2341 tatttgtgat gtcttgaagg actctgcaag gagtgcttca aacctggcaa ggcagaagaa
     2401 cataaaaatt gaatacagtc tggacaggga tgtgttttta ataaacgggg attatggccg
     2461 tcttcgtcaa atgtttttga ctgttataga taatgcaatt aaattttcct atgagggcac
     2521 aagcatttat attacattaa aaaacaatcg aataactata aaagatgaag gaacaggcat
     2581 atcagaagga gaccttccat atatatttga caggtactac agtgcaaatt ccggtgaaaa
     2641 caaaaccggg accggactgg gacttaccat agcaaaacaa atagccttaa ggcataatat
     2701 tgagatactt gcagaaagcc ggcccggtga gggaacaagc tttgtgtttg ttatccccgg
     2761 tgatttagaa taatatataa ttgaaaatct atacaacaaa atatataatg aaatagcatc
     2821 atatcattgc catgagctca gaatttattc gagaatacag gtatgggcac taccgggaga
     2881 gtctcagttg caggaggtat gtccactaat agaaaatctg tatgcgaaac tgcataatct
     2941 catatgttat aaaaactaaa aagtcttgga ggtaaaatgt atatggaaat aattaaagca
     3001 ataatactgg gaattgtgga gggcataaca gaatggctgc ctataagcag tactggacac
     3061 atgattttag ttgaggagtt tataaaattt gatgcttccc aggaatttat ggaaatgttc
     3121 agggttgtca tacaattagg tgccattatg gctgttgtac tcctatattt ccacaaactg
     3181 aatccctttg ctccaagcaa gacacagaaa caaaaaagag atactgtgga catatggttt
     3241 aaggtaataa ttggtgttct gcctgcagct gttctgggat tcctgtttga tgactggttt
     3301 gatgaaaatt tctataacta tcaaacagta gcagtaacat tgattttata tggtattttg
     3361 tttataatca ttgaaaataa aaataaggga aagcaggcag aaataaagag ttttaaggac
     3421 ctatcctata aaacggcatt gatcataggt ttttttcagg tattgtcact ggtaccggga
     3481 acttctcggt ccggggccac catattaggt gcaattatac ttggcacatc ccgtaatata
     3541 gcagctgaat attcattttt cctatcaatc cccgtgatgt tcggtgcaag tgctttgaag
     3601 ctggtaaagt tcggttttga attcacaggt atagaactgg cagtattggt aacgggaatg
     3661 gtggttgcct ttattgtttc tgtaatagcc ataagattct tgataagata tattaaaaat
     3721 aatgatttca aagtgttcgg ctggtataga attattctgg gcatattggt attggcgtat
     3781 tttatgttta catagcatac agacgaaggg gacacacctt cattaaaaag aaagagggat
     3841 gccctttgtc ccaagggaga gtttttgttt tcatgaggaa tgtcccggct atatgttata
     3901 aataagagca gcccctgtca ttttggacct gttgggaatc atactctgga agaggggaac
     3961 ttttaattcg cacgggatgg caatttaagg agtggtttga ttgaatgatg aaaatataat
     4021 aaggataact gaaaaagcta agaagcatct taataaaaaa ggaaagctcc aggtttcaat
     4081 tgaaattccc gagaaccgcc taagcagcga aagtgtcctt gtaccaatac cggaaataat
     4141 tgcaagaaaa ccgaaagttc cggaaaacta cagattattc accgttgacg gaatagacgt
     4201 atatgtatcc aattcagtag caatgcctaa aaacgaagtt gttatcgacc tggacatatt
     4261 tttgggcata aaacttctca atttatcggg atttaaatca agctagggtg ataatatgaa
     4321 gagggtagtt ttcttggttg acatgaatgc cttttatata agctgcgaaa aaacacgccg
     4381 ccctgatctt gaaggcaagc cggcagcagt ggcgggcgac cctgtaaacc gcacgggaat
     4441 agtgcttact gccaattacg aagccagaaa atatggggta aggactgcca tggttttaag
     4501 ggaagcgttc cggctctgtc cggacttaat tacaattcct cccgaccata gtttctacga
     4561 aatgaaatcc aaagaagtga tgaagatatt gtcagactat actccccttg tgcagcagaa
     4621 cagcattgat gaagcctggc ttgatatgac aggctgcgaa attctttttg gtaagccttt
     4681 ggaatcagca aaaagtataa tggagagaat aaggacggag cttgatttat ggtgctccat
     4741 aggaatatca gagaataaat ttttagctaa aatggcttct gaaatgaaaa agcccttagg
     4801 cataacagag ctttgggtaa aagatattca ggaaaagatg tggcctctgt ctgttagcta
     4861 tatgtacgga gtaggcaggc agacagctgc caagcttaat gaaatgggta tttttacaat
     4921 taaagactta gctctggcaa acaaggataa tgtatacaaa agactgggta aaaccggtgc
     4981 cgaactggtc cttttagcaa atggcataga tacaagccct gtggagcaaa acaaggaaga
     5041 tatgaaatcc atcggcaggt caaccaccct gccatgtgac atagttgaca ttgaatatgc
     5101 aaaatcaata ttgatggagc tttcagatga tgtgggcatg actgcaagaa aacacgggaa
     5161 aaaaggacat actgttcaaa ttactatcaa gtattcgaat tttaatacaa taaccagaca
     5221 gacaaccata aatccaacct gcaatgtgaa ggacatatat gctgcaggag agacacttct
     5281 tgagaacaac tggaacaaag agcccgtccg gcttttggga ataagcctca gcggctttga
     5341 taaaaatact cagcagatat cactttttca ggtaatggat aaaagcgaag aaaaacagga
     5401 tgacaaaatt gacaacctgg aagaaactat tcataagctg cgtcaaaagt atggaacaga
     5461 gataattaag ccgggcatac atgttaagaa agaagaatag gcaatagggg tttccaaatt
     5521 atgaggtcag gttcaaataa accgttaatg aaacaacgga cacaaggatt aaaaggaagt
     5581 aatggttaaa tgggaaaatc aaacgagaaa caaagagcat ggaatataat atggagtgtt
     5641 tcaaaagact actctcttac accggatatt acggcatacg atgaaagagg caaggcagac
     5701 ttatattgga attatataat cggtgccgtt tataaatatt ttgactacag gcttttaagc
     5761 gaattcttcg atactctcag gtatgacagg gactatatct tttatcagaa aataatgatg
     5821 cttggactgg aaggctgcat ctttgaaaaa gacagtaaaa acagaccggt attaaccggt
     5881 cttcgttctg agtacggtaa aaaggcatat gaaaacaggg acgaagctga attattcgat
     5941 gaaatccaag ctgcatactt cagcagggta aatggctata ccttaaaaat aagggataat
     6001 ataaacagcc tgctggatga cctgaatttt gacatcagtc ttggtaccgg acaattagtt
     6061 tttaaaatgg aacaaataat aaagaaatat ttcgaacctg aatataatga tgaattttat
     6121 attaaaaaga taaaaagaaa cagactatta cattttttcg gaagaaaaca cccttcaata
     6181 agaaggtcaa tcaagggagg caccttaacc cttataggta acgatgaagg taaaggcgca
     6241 aagggcggta aaatattctc aggcctgctt aagctaaaag aaaaatctga caataaagag
     6301 agagaattca ttaaagatta tttcggaatt tccaccatag gagaaacaaa tacgaaggca
     6361 ttggaaaagg tgctctgtga tgaagctcat gaaagctgcc accttcattt tacaagggga
     6421 caatttgatg acaccgacga tgctattttc aggcagaaac aaacctctgt acaaagagat
     6481 aaaaataaaa aacattatat caaaaattat atacggaaca caaacaacat tgctaaactg
     6541 acacaaagaa taaaaaacgc aataattgtc aattacacct attcaatgaa atctgaatcc
     6601 ggaaagctgg caacagaaaa aacatggaga accatactga atgacagcaa gattttcatt
     6661 aaaatcatca aagatgaaat gccggatatc tctgttgata ttatgcttga tgcttcagct
     6721 tcccagctta accggcagga aaccatagcg gaagaaggtt atataattgc cgaaagcctt
     6781 acgcgctgca atattcccgt aaggatttat tctttctgca atatgagaga ctacacagtc
     6841 atgaatcttt tccgtgatta caatgaaata aacaacaacg acagaatatt cagttactgt
     6901 gcagcaggtt ttaacaggga tggaatggca ctaaggacag ctcttcatat gatgggggat
     6961 tcaaaataca accacaagat tttaattgtt ctgtcggatg tgaaaccaaa tgatatgctg
     7021 ggcgtggctt ctgaaggatt tttaccgctt cagaaagaat attcggctga aaccggtgtg
     7081 aacgatactg caaatgaggt taggaagggc tggaaacaag gagtttccat tctgtgcgtg
     7141 tttactggaa gagatgagga tattccttcg gttaaaaaaa tatacggaca aaagttcacc
     7201 cgtatactga ccctggataa gtttgctgaa atggtaggcc tgctcataga aaaagaatta
     7261 ataaacagat aaaggagaac atattgaaaa aattactgcc tttaatgaag aaatatatga
     7321 aatatgcagt tttatgtcca atactaatgg tattggaagt tgcagcagat atagcaatac
     7381 catatctcat gtcaagaata gtggacgtgg gcattgcaaa tcaagatatt aaatatgtca
     7441 tacaaatcgg gcttatcatg attattgcag cctttttagg catgaccttc ggagtaatga
     7501 gttcacatta cggagcaaag gccggttttg gctttgcctc agaagtaaga atgaaagcat
     7561 ataaaaagat acagggcttt tcttttgcaa atttggacaa atttacagtt gcatccctga
     7621 ttaccaggct tacaaatgac tgcaacacat tggggcaggt aaccatgatg agcttaagaa
     7681 tggctgtaag ggcgcctttt atgatgatat ttgcactctt gatggcaatg aacataaatg
     7741 cttccctggc gagtgtattt atggtttcca tacctgtcct ggtccttgca ataattataa
     7801 tactaacaaa agcaaggcca ctttttttga agatgcagac aagggttgac agagtaaatg
     7861 caatcatacg tgaaaattta actgcaataa gagttgtaaa atcatttaac agacaaagtc
     7921 atgaagaaaa aagattcaag gagagaaatg attcattaat ggacactgcc ttaaaggcaa
     7981 ttactcttat tattttttta atgcctgtat taaatataat catttattct acaataatcg
     8041 ctgtactctg gtttggaggc agacaggttg ttgcaggttc aatgggaagc ggagaattaa
     8101 tatccttcat tacctatata actcaaattt taatgtcatt tatgatgatg tccatgtttt
     8161 tcatgcagct tcttagggct tctgcttctg tcacgagaat tgtggagata ctggatactg
     8221 aatcagaaat taaggaagtt gaaaatcccg taaaagaatt gacagacggg tctgtatcct
     8281 ttaaagacgt atgcttttgt tatcctggaa gcaccgaaaa aacattaaaa aatattaatt
     8341 tccatataaa ttcaggcgag gttttgggaa ttatagggtc caccggctca tcaaaatcaa
     8401 ccttggtaca gctcatacca agactttatg atacaatgga aggcagtgtg gttgtaggag
     8461 gccgggatgt gaaggattat gatatcaaat cgctccgcga ccatgttgct tttgtgctgc
     8521 aaaacaatac cttgttttca ggcagcatac gtgaaaatat gaaatgggga aatgaaaatg
     8581 cagaagatga agagattata aaggcgctta agttagccca ggcttgggaa tttgtatcag
     8641 cttataagga cgggcttgac cacaggattg agcagggggg ggataacttc tccggagggc
     8701 aaaaacaaag gctgacaatt gcccgcgccc ttttaaaatc tcctagaatc gtaattttag
     8761 acgactcgac cagtgcggta gatatggcaa cagatgcaaa aattcagcga gtttttaaga
     8821 atgagctgag tgatgtgacc acaataataa ttgcccaaag agtatcctcc atccagcatg
     8881 cagacagggt gcttgttatg aatgagggag aaatagagtc tttaggcaga catgaagacc
     8941 tgcttgtcag ttcgcccgta tacagagaaa tatacgaatc tcagcagaaa ggggtgcgac
     9001 catgaaacaa aagcagttaa gacctagaga ccctaaaaaa acacttttaa gattattctc
     9061 ctattttaaa tataatttag cattattttt tggaggcatt ttctttataa taatcggctc
     9121 cctggctgaa atcggaataa acggaatgct gagcccagtt attgacacat tagttggaga
     9181 ccatgaccgg ggacttttca taaaatatct tcttattatg gcggcaatgg cagcagtcgt
     9241 ttcttctgct caatatataa gcaacctgtc catggcgaaa ttagcacaaa agacagttca
     9301 taaaatacgt gaggacatgt tttcacatat ggaaaaactt cctgtttcat attttgacaa
     9361 acactctcac ggggaattaa tgtccacctt taccaatgat gtggatatgc tcaatcaatc
     9421 tcttgagcag agtgtttcac aaattcttat aacatctgta acagttgtgg gtacatttat
     9481 aatgatgctt gttttgagcc cggttttaac ggctgtggtg gcggtaatgc ttgcggttat
     9541 gctctattca gtaagatttg tagggaaaag atctgcagaa aactttcgta tgcagcaggc
     9601 tgcactggca gatatgaacg gttatatcga agaaatgatg tcaggccaaa aggttgtgaa
     9661 agtatttaac tatgaggata gagccataga cacattcagc aagaaaaatg aggaattaag
     9721 gaatgcaggc accttagctt caacctacgg agtaatgatg atgcctttaa tgggcaacct
     9781 gtcctttgtc atgtatgcct tgatatcaat ggcaggagct tatctggtta tgattgacct
     9841 ccttagcata ggtaatatag catcattttt gcagtataca aggtctatat caaggccgat
     9901 aacaatggtt tcaaatcagc ttaacacatt atttgctgca ttggcaggag cagaaagaat
     9961 atttgacgta ttggatgaaa aatttgaaac agacggggga gatgtcacct tattaaaaga
    10021 ctgcgaagga agaaaaaatc cttgctggat ggttccaaag actgacggag gcaatgatga
    10081 agtaccactg aagggattta ttgcatttaa agatgtggac tttggctata caactgaaaa
    10141 gaaggttcta aacaaaataa acctctatgc aaaaccagga caaaagatag cttttgtagg
    10201 ctcaaccgga gccggaaaaa caactgttac aaacctgata aacaggtttt atgaaataaa
    10261 tgagggtgtt atactttatg atggaataga tataaaaaga attaacaaat ttgacctgcg
    10321 aagcaccatg agcatggttt tgcaggatgt tcatttgttt gagggaacag tgaaggaaaa
    10381 tataaggtat gggagactgg atgccacaga tgatgaggta gtggaagcag caaggcttgc
    10441 aaatgcagat tactttatac agcatctccc tgaagattat gatacagtgc ttacttctga
    10501 cgggcaaaat ttatcccagg gggaaagaca actgttatca attgcaagag ctgcaatagc
    10561 cgaccccata attcttatac ttgatgaggc tacgtcttcg gtggatacaa gaacagaaaa
    10621 gctgatagct gaaggcatgg ataaattaat ggcaggaaga actactttcg tcatagccca
    10681 caggttatct accgttcgtg atgcaaatgc cataatggtg cttgaaaacg gagaaataat
    10741 tgagcggggc gaccatgatg accttatggc tcaaaaggga cggtattatg aattaaatgc
    10801 aggaaccata gaattgactt aaaaagcaaa caaaattgtg cagcgagact gtgagatggc
    10861 tgcgaatcct aaaagaaata caaaacgaaa aaacactaac ggagatattt atgaattacg
    10921 gttggataat ttaccctaaa gatactataa ataataaatt cgggaataat gcatttgaat
    10981 ggatgaaaga aagcgcattg agaaatgaca tcagaattga tatcattttt agcgatgact
    11041 tgataattct aaacaacaat aagaaaacag aattcatgta tttgggaaag accttaagcc
    11101 ttccggattt tgtcattatg cgagactata attatgctgt aagcatgcag cttgaaaatc
    11161 ttggcataaa ggttgttaat tccaccgggt ccatgtttaa ttccagaaac aaggcagtaa
    11221 ctgcccagct tttggttaaa aacaatgtaa gtacaccgga ctttttattt acaaaagaaa
    11281 agaactacga attaattcat aattacttta acggaagaaa atttgtaatg aaaactctag
    11341 aaggatccca gggcaaggga gtttatctga tagaaaacga agaagattac aatagtacat
    11401 acctggaaat aaatggtgaa tacttttgcc aggagtttat tgactttagc tgcggaatgg
    11461 accttagagt atatgtgctt gggaacaagg tcctgggatg tgtaaaaagg atttctgaca
    11521 gcagctttaa atccaactat tccttgggcg ggcgggttga aaaatttgaa ttgacagatt
    11581 caatcaagga gatctctcta agtgccgcca aagctgtagg acttgacttt tgcggaatag
    11641 acttgctttt taccaaggat tcattcacag tatgcgaggt aaacggcaat gcaggattta
    11701 gaactatcac taaggtatct gatatagaca taccaatgga gcttttcaag tggataaaga
    11761 gaagtttgta acaaatgtta caataatttt gtggatactg tgttaaaatt tattatgact
    11821 atcctaaact acaaatcaaa tatccatttg ttaacataat gtaaattatg ttacctgaaa
    11881 cgattatatt tattaacata atgtaattta tgtcaactga aacaagagat gaatataaag
    11941 gagaaaccat gatagatata aaagatacat tgttcaacat taccgaaaaa cacaaggaag
    12001 cagcagaact tttaatatct ttgggatttg acaatttaaa ggatgacaat cttagaaaga
    12061 tttttggaag ttctgtttca ttggaaaatg cattgaaata taagaagata aatgtggatg
    12121 agttcgtaaa tcagttaatg gaaagaatta aagataatga tacagctaag aacaaatata
    12181 aggacacaat aaaaatatcc ggagtgcttc cttgccctgt taaagttcct ctgatggaaa
    12241 cttttgaaaa gtggctgggt gagcaagact ttgatttcaa tttaaactat gatttaaaag
    12301 ctgcatccat gggtcttggc tggataaagg atgagcttaa aaattctgac aaggttccgg
    12361 atttgctcat atccgcaggc tttgacttgt tttttgacaa aaacctttta ggaaaataca
    12421 aatcagaaaa cctctttgaa gatattacgg gattacaaaa atataaccag gatttcgaag
    12481 aatataatct tgaagaccca agcggccaat attcaatgct aggcattgtt cctgccgtat
    12541 ttcttgtaaa caaggatgag cttaaaggca gaaaagcccc ccaaagctgg gaagagttaa
    12601 tgtcaggtga atatgaaaac aaggtgagcc ttcccattag cgattttgat ttattcaatg
    12661 ccttattgct taatatttgc aaactgtacg gagaggaagg cataagaagg ctggcaggaa
    12721 tattccaaaa gaatatgcat ccttctgaaa tggttaagtc tcatatgaaa agagaaagac
    12781 ctgctattac cataatgccg tattttttca cctggatggt tagggaagga agccctatgg
    12841 aattggtatg gccaaaggac ggagccataa taagcccaat tttcatgctc agcaaaaggg
    12901 aaacaaagga taaaacaaaa gtaatagcgg acttttttgc ttccagagaa gcaggagaaa
    12961 tcttatccca caagggcaag atgcctagcg taaatccaca ggtagacaat cagattccaa
    13021 aggaaaacaa atatatctgg cttggctggg atttcataaa ggaaaatgac atttcagact
    13081 taataaagaa atgcgagaat atattcaatg aaacacttaa aggagatgac ggaatatgaa
    13141 tttagtaata atatcaggac ctccttcctc aggtaaaaca tcagtaattt taaaaacaat
    13201 agaagcaatt aagaaaagag gtataaatgc aggggtagta aaatttgact gcctttatac
    13261 agacgatgat atattgtatg aaagggcggg aatccaagtt aagaagggac tttcagggtc
    13321 tttgtgccct gaccacttct ttgccagcaa tattgaagag gttgtaagat gggggaagaa
    13381 ggaaggcctg gaattattga taactgaaag tgcaggactg tgcaacaggt gttcacctta
    13441 tattaagaat atcaaggctg tctgtgtaat tgataattta agcggtataa acacacccag
    13501 aaaaatcggc cccatgctaa aaacggcaga catcgtagta ataacaaaag gagatattgt
    13561 ttctcaggca gaaagagaag tatttgcatc aagagtcaat tctgtaaatc ctcaggcagt
    13621 aattatgtct gttaacggac ttacaggaca gggggcattt gaactgagca cattgattta
    13681 tgacaaaaat gcaaatatag aaacagtaaa gggcagtgag cttaagtttc ccatgccttc
    13741 agcgctttgc tcatactgcc tgggagaaac aaggataggc gaagaatacc agatggggaa
    13801 tgtgagaaag atgaatatag gagaagaaaa tgacaagcat tagagaattg gaaatgaaaa
    13861 aaatcaaaga cctggaagct caatatcctt ttgtattatc tttctttgaa aacaataaat
    13921 taagcgttga aggctttaag gattcaacat ttattgaata tttaaaccat tttacccagg
    13981 aagaaatcga agaatgggca ataaacatcc ctaagataaa atccgacctg gaggcttatg
    14041 taaaccaaat gcttcagttt ttaggcctgg aaaaagaaac tgtagattca attactattc
    14101 tggcaggcag ggataaatac ggcaacagag aaaactttaa tgaattgaca attaataaaa
    14161 gtgaaattgt ttcaatagtt ggaccaacag gttccggaaa aagcaggctg ctggcggata
    14221 ttgaatgggc agcgaacgga gatacaccta cagaaagggt ggtcttgata aacggggaaa
    14281 aacctgatta cagtatgaga ttttcaacca gcaacaagct tgttgcccag ctgtcccaaa
    14341 atatgaactt cataatggat ttaaccgtca gagaattttt ggagcttcat gctaaaagca
    14401 gaatggttga aaaccaggaa gaagtagtct taaaaattgt ggaagcagcc aatgaattag
    14461 ccggtgagaa atttgatccg gatacacctg taacaggcct cagcggaggg cagtcaaggg
    14521 ccttaatgat tgctgatacg gcaatattaa gcacctcacc catagtattg attgatgaaa
    14581 tagaaaatgc gggtattgac agaaaaagag cattaaatgt gctggtcaat gaagaaaaga
    14641 tagttcttat ggcaacccac gaccccagtc tcgctttaat ggcagataaa agaattgtta
    14701 taaaaaacgg aggaatccat aaggttatag aaaccggcac caaggaaaaa gaagttttaa
    14761 aggaactgga aaaaatggac aaaataataa attccatgag aaccagttta agaaacggcg
    14821 atagtattgc ttaatttgaa aatttgtatt agaatatagt atgaaataaa agggggcact
    14881 tgttgtgaca aaattaaagg ctgacttatc actcttgatg gttacgcttg gatggggcat
    14941 atcatactac ttaactgatt tgagcctgga agacatgggt ccattcactt taaattcaaa
    15001 cagattttta atagcatttg ctgttgcttc tatacttgct tttcctaaac taaaaaatat
    15061 ttcaaaacat acatttaaat tcagccttat tttgggtgtg gcactggtat ttgtttacct
    15121 gggggcaacc tttggcacta aatacacttc attgtcaaat gtaggttttt tatgcggact
    15181 caccgtaatt ttcaccccta tattatcggc tgtaatttat aagaaactgc caggcaaaaa
    15241 acaaacatta gtaattctat tgagctttgt gggtatagcg ctgctgacat taaaagatga
    15301 tttctccata aactatgaca atttattggg agacctttta tgtattatgt gtgcttttgc
    15361 ctatgccgtc catcttttaa tcaccgaaaa ggcagtgaca tttgaagatg taaatgcctt
    15421 tcatctggga gttttacagc ttggcaccac aggcgtattt actttaatac tggcccttat
    15481 atttgaaacc cctcatttgc ctacagaacc tagatactgg ggacctgtat tgtttctatc
    15541 gattttctgc acaggcatgg catttattgt tcagtgtgta gcccagcaat atactgaagc
    15601 atcccatgta ggtataatat tctccttgga aactgttttt gccgggattg ttgcatttgt
    15661 gcttgcccat gaagttcttt cattccagtc ctatgtaggg gctgtactta tggttgccag
    15721 tatatttatc atggaaattg atttttcaaa attcagatta tgcaggaaaa atcaaaacga
    15781 accggaataa tcttccggat tggatttata acactgaggg tttgcaggca gctctctttt
    15841 ttttgtaaat ttttatagat tttacattta tactcttaat aaatatctaa aacatacaaa
    15901 taatataaat aatatttgat gaaaggatga tattatgccc attaatgata atgttcatga
    15961 gcagatgcaa aactgggagc ttcagcaaaa gcaggaaagc caaaaggaat taagagataa
    16021 atatgaatat atgaatgata aatatcaaaa gatgaaaaat gattatacca gtttcaaaag
    16081 tgcccgaagg gacaaaaatg aggaaatata caacaacagc agcgaccaaa ccggcaaaaa
    16141 cccatatatg cggtatgaat taaacaatac cttcaaaaac aaggacgata aaaaagaaag
    16201 caaataaaga agagaaaaaa ggcaaatatt cagttttgag tatttgcttt ttaaaatttc
    16261 cccttgacaa attacaaatc atgttatagg attatattgt aatcattaca attaaaaaat
    16321 gattataaag agagctaata tgaaaacact ggaagaatta tcagatgaat taaaaaagaa
    16381 taatataagg cttacacatc aaaggctgaa ggtattggaa tacttgtcaa acagcaagga
    16441 ccatcctacc gttgagcaga tttataatgc tctcaaaagg gaagttccgt ccttgtctaa
    16501 aactacaatc tacaatacat taaattattt gtcagagctg aatcttataa aggttctggc
    16561 aatagaagaa aatgaggctc attttgatgc tgtcaaggaa agccatggtc attttaagtg
    16621 ca
//
LOCUS       DAOPUT010000028        16532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3552_length_16532_cov_17.367239, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000028 DAOPUT010000000
VERSION     DAOPUT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16532
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3552_length_16532_cov_17.367239"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..579
                     /gene="ndk"
                     /locus_tag="PKK75_04060"
     CDS             163..579
                     /gene="ndk"
                     /locus_tag="PKK75_04060"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNZ82800.1"
                     /translation="MERTLVLIKPDAMQRKLMGEIISVYEKSNLHIAAIKIVKATIDV
                     AEVHYEEHKEKPFYNELIDYITSSEVCALIIEGDNAVDDVRRLNGATDPKDADPSSIR
                     GRYALSKSENSVHASDSITSGEREIKIWFPEVYAGN"
     gene            582..1226
                     /locus_tag="PKK75_04065"
     CDS             582..1226
                     /locus_tag="PKK75_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNZ82801.1"
                     /translation="MSIIDVFYQNRNEELAVPMAKYMKNKFTFLGIKTPERRELARDF
                     ISESKKAGEIDWDFIYKCFELPEREFQYLALYYMDTVKKLFTVEDMDRIEGLITNKSW
                     WDSVDSISPLVGYICLKYPNIKEEVIDSWIFSDNIWLKRVSILFQLKYKEKTDREFLA
                     KAIVNNSNTSEFFVNKAIGWALREYSKTDKNWVRDFIKNNELSQLSIKEGSKYI"
     gene            1335..1988
                     /locus_tag="PKK75_04070"
     CDS             1335..1988
                     /locus_tag="PKK75_04070"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HNZ82802.1"
                     /translation="MTIQILFVIALASGLLILYPRKAYAHCDTMDGPAVKDGEKALET
                     GNINYAYKWIFEEYEEELKEIFELALKARKLGQYAKEVSDRWFLENFVRIHRAGEGAS
                     YEGLKPSGFELEQVVVAADKAIEIGNMSPLKGLVTDEEYHALEEKFDKAMSLKDFDIN
                     DVKASRAYVNAYVTFFKMAEGEEHDHDHGHAHGHDQSHEPEHVHELVHGHDTEHHSH"
     gene            complement(2158..2508)
                     /locus_tag="PKK75_04075"
     CDS             complement(2158..2508)
                     /locus_tag="PKK75_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82803.1"
                     /translation="MKKIIALVLALTYTLVLVSCTSIDNTQTDHQTLTIEKVKKLAEK
                     GEDLTWSDFERYPSEDIGSGLCIYRYEIDRDYYLLIGGGSTELPPMYIRLVSAKDRNS
                     YIDIRTENIDDFIK"
     gene            3053..3529
                     /locus_tag="PKK75_04080"
     CDS             3053..3529
                     /locus_tag="PKK75_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNZ82804.1"
                     /translation="MKTAALIITIIMFIIGIAGTILPALPGVVLIFAGMVIYGFMTDF
                     ASLGLWFFVLQLLAMTVIFVVDFIASAVSTSKYGGSKQAAFGAAAGTILGIIFFGPLG
                     IVLGPFAGSVAAELLLGKEIKQAVRVGFGSLVGLLGGTIFKLAAEIVMIIYFFMSI"
     gene            3773..4333
                     /locus_tag="PKK75_04085"
     CDS             3773..4333
                     /locus_tag="PKK75_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003329273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HNZ82805.1"
                     /translation="MINGKITKKRFFEIMECLDKKLAENNIELSINIYGGTAMMVCFD
                     IRSATKDIDALFDTSPQLNNILSDIAEMYDLDSEWINQAIKEPLKHLKTEDLKVLYKF
                     NNLKIFAPSAEQMLAMKILSSRPEPYKDFEDAEFLIQYLKIDDLDNVIKIFDKYAGRK
                     YLGDRQKIFLNYIGKDLNKRWEEFKI"
     gene            4312..4689
                     /locus_tag="PKK75_04090"
     CDS             4312..4689
                     /locus_tag="PKK75_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82806.1"
                     /translation="MGRIQNIIDAEDFNYSLINLLIDLYSSNYENHFNGLYFDEKKII
                     ELNETEKDKLTKLAAIIEYVGNRRENAKLYDWIYSSELKLENPYTPGVNEESFARLKR
                     IITAPREFSSRNVFYDYTTIKPV"
     gene            4843..4998
                     /locus_tag="PKK75_04095"
     CDS             4843..4998
                     /locus_tag="PKK75_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82807.1"
                     /translation="MTKMDNNKLGCLIIHGFGGGIFEIESLADYLTERGYIAACPELK
                     GHTGLDG"
     gene            5150..5869
                     /locus_tag="PKK75_04100"
     CDS             5150..5869
                     /locus_tag="PKK75_04100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNZ82808.1"
                     /translation="MKLNLFITAITPGIAIALVFYWVDRHDREPLSMLLKVFIFGALY
                     VFPTILIENFLLMFNIFGGVPGAAYTAFIVAGLTEEYMKRKVVLKYVYFNPVFNEKLD
                     GIIYCVMAALGFATIENIMYVVFAFSDVESVGYYRAIISVPAHMLFAVTMGYYLSLAK
                     FSSTPEEHLHYFKKSLSVPALMHGIFDFILMANIPMLMVFFMPFVIYLWVGNLKKLNV
                     YYNDSKYYANKENDFDDFDEI"
     gene            5890..6795
                     /locus_tag="PKK75_04105"
     CDS             5890..6795
                     /locus_tag="PKK75_04105"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNZ82809.1"
                     /translation="MAFFKIKGVIPPMITPFDEHDMVDYDKFTENILKWNEYDLAGYL
                     VLGSNSETVYLTEEEKLKLVQITVKHRKKGNIVIAGTGMESTKATIDLTNKAAELGAD
                     CALIITPYYYGDKMNDEALINFYTEVADGVKIPIMIYNVTKYTHVNISPYAVGALSKH
                     PNIIGMKDSSGNVSQLIDFKRAVGDEDFNLMVGTASIWYPALTLGVEGCVLALANCCP
                     GECIEIQKEFVDGNLEKARELYERVFPVNTAVTAKYGIAGLKYACDLLGFQGGYVRKP
                     LIQLKEKEKAELREILKKAIPSLRV"
     gene            6920..7339
                     /locus_tag="PKK75_04110"
     CDS             6920..7339
                     /locus_tag="PKK75_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNZ82810.1"
                     /translation="MSKKIKVKPSKGQSTAGFFVGIVFCLIGLFVAIPIFGPFGIFWT
                     MIAVIITVMNGKNAFTEEGVSTHEIIIEDNDTIKDSNLMKGINIVKDNAESLKDEDYV
                     NISDSKKRLEQIKVLYENGFMTREEYEEKRKSIIADI"
     gene            7645..8409
                     /gene="pstB"
                     /locus_tag="PKK75_04115"
     CDS             7645..8409
                     /gene="pstB"
                     /locus_tag="PKK75_04115"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNZ82811.1"
                     /translation="MKIKTESLNLFYGSFQALRNINMEIKDRSITAIIGPSGCGKSSL
                     LRLFNRMNDLIPGVRVEGKINLDGVSIYDKNMDVVELRQKVGMVFQKPNTFPMSIYDN
                     IIFGPKHLGQKNKKELDQMVEHSLREVALWDEVKDILNKSAFELGAGEQQRLCIARAL
                     AVKPDVILMDESTSALDPESTLKIEDLMQELSKNYTIIIVTHSMEQAARASDMTAFMM
                     MADDRAGTLVEYAETEQLFSNPKDKRTEDYITGRFG"
     gene            8429..9253
                     /locus_tag="PKK75_04120"
     CDS             8429..9253
                     /locus_tag="PKK75_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ82812.1"
                     /translation="MNCKIRKQMKATVMAAQLLVFMILISACSRNNEVGVIVAGSTSV
                     QPYAEVLAEEYMILNPGTEIDIQGGGSSAGITAATNGTSDIGMSSRELKDKEKSLNSI
                     EIAKDGLVVIVNPKNPIQDLTLDQVRDIYSASITEWNKLGGTDSKIHVITREEGSGTR
                     SAFEELLMGESEITPKAIVQDSNGSVRLLVADDPNSIGFISMGIVDETVKALSLEGVT
                     ATRESILDGSYTLSRPFLFVTLNEPTGQTKHFIDFVLSDEGQKLLSHEGLVPSMNK"
     gene            9267..10112
                     /gene="pstC"
                     /locus_tag="PKK75_04125"
     CDS             9267..10112
                     /gene="pstC"
                     /locus_tag="PKK75_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNZ82813.1"
                     /translation="MKNIDEKKYEILFLIIALSSLTALALITGFIFYKGLPIIAKVGL
                     FDFIFGMKWAPGKGEFGIFPMIVGSVSVTLGAAIIGVPIAICCSIFLAEFAPVTMRNI
                     FRPAIQLLAGIPSVVYGFWGVLFIVPFIRNYLGGPGLSILAGSIILAIMILPTIISIT
                     EVSLLALPRHYKEGALALGLNQWQTIYALLLPAAKSGIVSGIILGIGRAIGETMAVIM
                     VLGNSVAIPESILDPVRTLTTNIGIEMGYASGEHQQALFATGIVLFVIIMILNASAQY
                     ITRKR"
     gene            10120..10956
                     /gene="pstA"
                     /locus_tag="PKK75_04130"
     CDS             10120..10956
                     /gene="pstA"
                     /locus_tag="PKK75_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNZ82814.1"
                     /translation="MKINPRTSQKIAKFIIWLAAILVLVVLFAIITYILAKGIPHLSF
                     GFLTEIPRNMGREGGISSTIVGTLLVTALAVIIATPFGIGTAVYLTEYTRESRVTRII
                     RFSAESLAGIPSIVYGLFGFIFFVTYLKLGWSILSGGLTMAIMILPTIIRTSEEAIRT
                     VPNLNREVGYSLGATKWQTITSTVFPSALPGIANGIILSIGRCVAETAAVIMTAGSAL
                     RMPTSMFSPTRTMAVHFYILAREGISMENAYSTAAILIILIFLINVVFNMLVNRFVAK
                     GR"
     gene            10976..11740
                     /locus_tag="PKK75_04135"
     CDS             10976..11740
                     /locus_tag="PKK75_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNZ82815.1"
                     /translation="MDQQPKIIIKDLNFYYNKKQAIKNLSLDISKNEILAIFGPANSG
                     ITTLLRTLNRLSDLTYGARHEGDVLLDGENIYAPDINVTELRRRVGMVFDVPAPLPMS
                     IFDNVAFGPRMSSLINKKSDLSDKVEQSLRMAALWDDVKDRLDTPAMRLSGGQQQRLC
                     IARVLALEPEVILLDRPCSALDPVSTAKIEDSLKRLSEQFTIVIAPHNVQQATRIADN
                     VAFMLMGDLIEHGDAFEVLSNPKDKRTSDYITGRFG"
     gene            11801..12466
                     /gene="phoU"
                     /locus_tag="PKK75_04140"
     CDS             11801..12466
                     /gene="phoU"
                     /locus_tag="PKK75_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNZ82816.1"
                     /translation="MRNRFDKELENLNNEIIEMGTMIISAIENSVCALINQDMSTVKV
                     VNELENEIDRMEKVIESHCMRLLIQQQPVASDLRLIETALKMITDMERIGDNAEDIAE
                     IVKYFENKRFIKDLIHIPKMAEVTISMVKRSVEAFVNKDKDLALQVCYDDDIVDGLFI
                     TIKTELIEMIGKDSKNGEQAIDFLMIAKYLERIGDHAQNIAEWVIFAITGEHKNTDDI
                     GKQ"
     gene            complement(13107..13985)
                     /locus_tag="PKK75_04145"
     CDS             complement(13107..13985)
                     /locus_tag="PKK75_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNZ82817.1"
                     /translation="MSNFRPIEDKYSIEKEHWVGTAFRVRNYFPESKNLLDRFSPFIL
                     MDYNAPRIFPPSKSPKGVGPHPHRGIETVTLVFQGAVEHHDNAGNHGIIYPGDVQWMT
                     AGAGIMHKEYHEEEFRQRGGMFHAIQLWINLPKKDKYTAPRYQELSNESMGKVKLPND
                     FGEVTVIAGDFKNVKGPALTFSPVNIYIVDLIAEGSVTLNEPAGYNTGLFVIKGDPHI
                     NNTPCSENDFVLFENKEGEIEIKSMSKDSKIILLSGQPLNEPAVLVGPFVMNTKEELD
                     QALKDYREGAFGTEKF"
     gene            14159..14920
                     /locus_tag="PKK75_04150"
     CDS             14159..14920
                     /locus_tag="PKK75_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNZ82818.1"
                     /translation="MDDYIKSNYIIEEKIMIQEIPAIVFKPKDAGEKIPTAVFYHGWS
                     SNKETQRMRGFILASAGYQVVIPDAVYHGERNPLNDYGPEAAIEYFWYVVFKNLEEYS
                     VIEGDLVTKYGADPNRIAVMGNSMGGFTSGGIFTHYKKVKALIVFNGCCWWEDFNKKA
                     DVAITEKLEKVIKKVEELDPMNNLNLLTDRPILLLHGDSDTSVPVESQRVFYDRLSPM
                     YINKEKIKLIEYPKLNHVITTNMMEESINWLGRYM"
     gene            complement(15087..16472)
                     /locus_tag="PKK75_04155"
     CDS             complement(15087..16472)
                     /locus_tag="PKK75_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNZ82819.1"
                     /translation="MDFKYVAEPFRIKMVEPLKMLTREEREQKIKEANYNMFNLKAED
                     VYIDLLTDSGTNAMSSDQWAGVMVGDESYAGGKSYFNVIKAGQDIFGYKIIQPVHQGR
                     AAEKVFFPILVSKGKYAIANMFFDTTRAHVEIAGARAIDCVVEEAKDPKVRAPFKGNM
                     DVEKLEKLINELGSENVGVVVMTITNNSAGGQPVSVQNMRDVSAVCKKYGIPLDIDAA
                     RFSENAYFVKQREEEFKNWSIKDITREMFSYADFFTMSAKKDAIINMGGLIGIKEDNQ
                     ALYEKVKANCISFEGFTTYGGLSGRDLEAMAIGLYEGQDEDFLRYRIGQLEYLAAKLD
                     NAGIAYQAPVGGHGLFIDAKDMFPHIPYYEFPAQALAIELYKEAGIRTCDIGSYMLGN
                     NPDTGEQLEADFEFTRLAVPRRVYTQAHFDIMAEALIAIKERAASVKGYRITWEPKIL
                     RHFQASLEPLE"
BASE COUNT         5422 a         2626 c         3381 g         5103 t
ORIGIN      
        1 gtgggcggct aaaattttag ccgcctaatt attataaatg tgtggggaca ttcctcctac
       61 aaaaatcgac ttttggatga gtaataatgg aataatcaaa ggctgataaa atttggtgag
      121 gaattttata catataaaat gattggccta aaaggagtgt atatggaaag aacattagtt
      181 ttgataaaac cggatgctat gcaaagaaaa ttgatgggag aaattatttc tgtttatgaa
      241 aaatcaaact tacacatagc agccataaaa atagtaaaag ctacaattga tgttgcagaa
      301 gtacattatg aagaacacaa ggaaaaacca ttttacaatg agcttattga ttatatcacc
      361 agctcagagg tgtgtgcatt gataattgag ggggataatg ccgtggatga cgtgcggagg
      421 ttaaacgggg caacagatcc taaggatgcc gacccttctt caatcagggg caggtatgca
      481 ttgtcaaaat ctgaaaacag tgttcatgct tcagattcca taacaagtgg agaacgggaa
      541 ataaaaatct ggtttccgga agtttatgcg gggaattaat tatgagtatc atagatgtgt
      601 tttaccagaa tagaaatgaa gaattggctg tgcccatggc taaatatatg aaaaacaaat
      661 ttacattttt gggaattaaa actcctgaaa ggagggaatt ggcaagggat tttatcagtg
      721 aaagcaaaaa agccggggaa attgactggg atttcatata taaatgtttt gagctgccgg
      781 aaagggagtt tcagtatctg gccctttatt acatggatac agtaaaaaag ctgtttactg
      841 ttgaagatat ggataggatt gaaggattaa ttaccaataa atcctggtgg gacagcgtgg
      901 attccatcag cccccttgta ggctatattt gcttaaaata tccgaatatc aaggaagagg
      961 taattgatag ttggattttt tctgataata tctggcttaa aagagtatcc atattatttc
     1021 aattaaagta taaagaaaag acggataggg aatttttggc taaagctata gtcaacaatt
     1081 caaatacttc tgagtttttt gtaaacaagg ctattggctg ggcattgaga gaatattcaa
     1141 agacagataa gaactgggtg agagatttta ttaaaaataa tgaattgtca cagctcagca
     1201 ttaaagaagg tagcaaatac atataagccg acgacggcta tttattattt ctaaaattta
     1261 atataaatca aagattagga acctgaaatt gggtataatt ataataaaat aataaataaa
     1321 aaggagaagg taaaatgacg atacaaattt tatttgtaat agccttagct tcaggtttat
     1381 taatattata cccaagaaaa gcatatgcac attgtgatac gatggatggt ccggcagtaa
     1441 aggacggaga aaaggcgctt gaaaccggaa atattaacta tgcgtataaa tggatttttg
     1501 aagaatatga agaagaacta aaggaaattt ttgagttagc tctaaaagcc cgtaaacttg
     1561 gacaatatgc caaagaagtt tcagaccgtt ggtttttaga aaactttgtt agaattcacc
     1621 gtgcgggtga aggtgcatca tatgaaggtt taaaaccttc aggatttgaa ttggaacagg
     1681 tagttgtagc agctgataag gctatcgaaa taggaaacat gtcacccctt aaaggtttgg
     1741 taacagatga agaataccat gctttagaag aaaaatttga caaagcaatg tcccttaaag
     1801 attttgatat aaatgatgta aaagcttcaa gggcatatgt taatgcatat gttacattct
     1861 ttaaaatggc agaaggcgaa gaacacgacc acgaccatgg tcatgcgcac ggacatgatc
     1921 agtcgcacga accagaacac gtacacgaac tggtacatgg gcatgatacc gaacaccaca
     1981 gtcattaatt gaaattttga gcatcttcca cgtgtataaa cccgataagg ctccctacgg
     2041 cttgatgttg gttggtaatt ttttcttcgt catgggtata tctcctttga atttttgata
     2101 gaaaaagagc agccggcttc gactgctctt tatccgttgt gacattctac aaatttacta
     2161 tttgataaaa tcgtctatat tttcggttcg tatatctata tagctgtttc tgtccttcgc
     2221 ggataccaac cttatataca taggagggag ttcggtgctt cccccgccaa tcagcaggta
     2281 atagtcccgg tcaatctcat aacgataaat acataaacct gagccaatat cttcactggg
     2341 gtatctttca aagtcactcc aagtcaaatc ttctcccttt tcagcaagtt tctttacctt
     2401 ttctattgtc aatgtttggt gatctgtttg cgtattatca atcgatgtgc aactaaccaa
     2461 taccagcgta taggtcaagg ctaaaactaa tgcaattatc tttttcatat ataatacctc
     2521 caatcacatt tatctgccgt ttgtttttac gaacaatctc ctgaaatgtc cctgatatat
     2581 aagaattata ccatagcaat caagaatttt gtataacatc ttacttactt ataccatagt
     2641 aatcaagaat tttgtataac atcttactta ctggcaccaa gctgattagg ggattttttt
     2701 gtttttcaaa acaactgtac aagattatgt acagctgctg aataattgat atacatttta
     2761 gcaattaatg ggtataataa aaatgaagcc ttgttctata gactatgatt atgatgattt
     2821 catcactatt aatttatggt ataaataaag atgttgaaat agattaagat atgaaagaat
     2881 tcacgagaca ttggaaagag aaatagcaga aatgcgaaaa aatgctccag tgagtaaaga
     2941 aaagaaaaag gataccagat tataaatcat agtgattgat taggagattg ccataaaaca
     3001 agaaaatcct aaaacccttt aagactaaaa aataattggg aggagcaata acttgaaaac
     3061 agcagctttg ataataacaa taataatgtt tataattgga atagcaggga ccatacttcc
     3121 ggcactgccg ggggtggtgc ttatttttgc cggcatggtt atatatggtt tcatgactga
     3181 ttttgcaagc ctgggtcttt ggttttttgt tctgcagcta ctggctatga cagtaatttt
     3241 tgttgtggac tttatagcat cagcagtaag taccagcaag tacggcggga gcaagcaggc
     3301 ggcctttgga gcagctgcag gtacaatatt aggaataata tttttcggtc ctttaggaat
     3361 tgtactgggt ccctttgcag gctcggttgc agcagaactt ctccttggaa aagaaataaa
     3421 acaagcagta cgtgttggat tcgggtccct cgttggatta ctgggtggaa caattttcaa
     3481 actggcagct gagatagtaa tgataatata cttctttatg agcatataaa aaataatgga
     3541 atagctgttc tctatacaca ctttatatac tcttagattg ataaataatc ttgtaaaaat
     3601 aagaggatgt cctgcaaagg ttggaattat agcatattcg actatgaaag cgaaaatgac
     3661 aaaacagatg cggcaggagg atataattat atattaagag gattgttagt aaatattgta
     3721 aaatatcacg gaaagtgcta taatattaat ataaaagcaa gggtggtgtc taatgattaa
     3781 cggaaagatt acaaagaaaa gatttttcga aataatggaa tgtttagata aaaagcttgc
     3841 tgaaaacaat atagagctta gcataaatat ttatggtggt acagcaatga tggtatgttt
     3901 tgatattaga tctgcaacca aagatattga tgctctgttt gatacttctc ctcaattgaa
     3961 taatattcta tcagatattg cagaaatgta tgacttagat agtgaatgga taaatcaggc
     4021 cataaaagaa cctttgaaac atttaaaaac tgaagactta aaggtactat ataaatttaa
     4081 taatcttaaa atatttgctc caagtgcaga acagatgctt gcaatgaaaa tattatcatc
     4141 cagacctgaa ccatacaaag attttgagga tgcagaattt ttaattcagt atttaaaaat
     4201 agatgattta gataatgtaa taaaaatctt tgataaatat gcgggcagaa agtatttagg
     4261 ggatagacaa aaaatatttt tgaattatat aggaaaggac ttgaataaaa ggtgggaaga
     4321 attcaaaata taatagatgc agaggacttt aattattctc ttataaattt gcttatagat
     4381 ttatactcaa gcaattatga aaatcacttt aatgggctgt attttgatga aaaaaagata
     4441 atagaattaa acgaaacaga aaaagataaa ctaactaaat tagctgctat catagaatat
     4501 gtgggaaaca gaagagaaaa tgcgaagctc tatgattgga tatattcaag tgaattaaaa
     4561 cttgaaaatc cttatacacc cggcgtaaat gaggaaagtt ttgcaagatt aaaaagaatt
     4621 attactgctc caagagaatt ttcctccagg aatgtatttt atgattatac aaccataaaa
     4681 ccggtataat ctaaggacta tacaaaataa tgagaaagtt aaatggtaat taatattcaa
     4741 acgaaatccc ttaaagtttt ttaagggatt tttattgcgg ggatgcaggt ttgtgttata
     4801 ctgtaggaga agtgttccta aataaaggtg actgatgata tcatgacaaa gatggataat
     4861 aataaattag gatgtctcat aattcacggc tttggcgggg gtatatttga aattgaatct
     4921 ttggctgatt atttgactga aagaggttat attgcagcat gccctgaatt aaagggtcat
     4981 acaggtttag acggataatt ctgtgtaaat atggatattg aaagttttta gatctataca
     5041 atgaaaagga aatatttagc cggataattc taatagtaat tatggatatt gaaagttttt
     5101 agcccgataa tctttatgat aattaagata taatggaggc attttaacaa tgaaattgaa
     5161 tttgtttatt acagcaataa ctccaggaat agccatagca ttggtatttt actgggttga
     5221 cagacatgac agggagccct taagcatgtt gctgaaggtg tttattttcg gcgccctcta
     5281 tgtttttccc acaatactta tcgaaaattt cttgttaatg ttcaatatat tcggaggggt
     5341 gcccggagct gcctacactg cttttattgt agccggattg acggaagagt acatgaaaag
     5401 gaaggttgtg cttaagtatg tgtatttcaa tccggtattc aacgagaaac tggatggcat
     5461 aatatactgc gtaatggctg cactgggttt tgcaaccatt gaaaatataa tgtacgttgt
     5521 atttgcgttt tcggatgttg aatcagtggg ctactacagg gcaattattt cggttccggc
     5581 tcacatgctg tttgcagtga ctatgggata ttatctttca ttggcaaaat tttcttcaac
     5641 ccctgaagag caccttcact actttaagaa atcattgtcg gtgccggctt tgatgcacgg
     5701 aattttcgat tttatattaa tggcaaatat tccaatgctt atggttttct ttatgccctt
     5761 tgtaatttat ttgtgggttg gaaatctaaa aaagctgaac gtttattaca atgattccaa
     5821 gtactatgca aacaaagaaa atgattttga tgattttgat gaaatataag tgttggaaag
     5881 gagacaaaaa tggcattttt taagattaag ggagtaatac cacccatgat tacacccttt
     5941 gatgagcatg atatggtgga ttacgacaaa tttacagaaa atatattaaa atggaatgag
     6001 tatgatttag ccggctacct ggtgctgggc tcaaacagtg agacggtata tttgacagag
     6061 gaggaaaaac tgaagttagt ccagatcact gtaaaacaca ggaaaaaggg gaatattgtc
     6121 attgcaggaa ccggtatgga gtcaacaaag gctacaattg acttgacaaa caaggcagct
     6181 gaacttggag ctgactgtgc tctcataata acaccatatt attatgggga taaaatgaat
     6241 gatgaagcat tgattaattt ttatactgaa gttgctgacg gtgttaaaat tcccattatg
     6301 atttataatg ttactaagta cacccatgtt aatatttctc cttatgctgt gggtgccttg
     6361 tccaagcatc ctaatattat cgggatgaag gacagctcag gcaatgtttc acagcttatt
     6421 gactttaaga gggctgttgg agatgaagac tttaatttaa tggtgggaac agcatccata
     6481 tggtatcctg ccttgacact gggagttgaa ggatgtgtct tggccttagc aaactgttgt
     6541 cccggggaat gcattgaaat tcaaaaggaa tttgtagatg gcaatttgga aaaggcaaga
     6601 gaattatatg aaagggtgtt tcctgtaaac actgctgtaa ccgccaaata cggcatagct
     6661 ggtttaaaat atgcttgtga tttattggga tttcagggcg gttatgtaag aaagcctttg
     6721 attcagctga aggaaaagga aaaagcggaa ttaagggaaa ttcttaaaaa agcaattcct
     6781 tcgcttcgcg tatgacaacc cgctaggtgt ggacagtagc tgcaatggta tccggcagac
     6841 aaaatgctgc aattattaat tgtatatgga gacagttttt gatataatta attaacttat
     6901 caattgttaa ggagaggtta tgagcaaaaa aattaaagtt aaaccaagca aggggcagtc
     6961 aacagcagga ttttttgttg gaattgtatt ttgtttgata ggtttatttg ttgctattcc
     7021 aatctttggc ccattcggaa tcttttggac catgattgca gtcatcatca ctgttatgaa
     7081 tggcaaaaat gcttttacag aagagggtgt ttcaactcat gaaattatta tagaagataa
     7141 tgatacgatt aaagactcta atttgatgaa aggtattaat attgtgaaag ataatgctga
     7201 atcattaaaa gatgaagact atgtaaatat ttcagattct aaaaagaggc tggaacagat
     7261 aaaggttttg tatgaaaatg gttttatgac cagggaagaa tatgaggaaa aaagaaaaag
     7321 cataattgca gatatttaac atattaaaaa cgtcgttgca ggcgttttta taatgctata
     7381 ttttagaaac ccgggagcgt cgcctgttct tgctgccagg gcagttcaat ggacagccta
     7441 atgtaaatga cactttagaa tataatatcc ggcattaaat gtatcgggaa tccggccaaa
     7501 acagaatatc caggatactg gttgaagttg tctattaacc tcaaaaaatg atatacattg
     7561 tttttacatt ttgttataat gttgttgtgt taaagatata cttattttca tgacatataa
     7621 gaatgcaata agcgagggta atgaatgaag ataaaaactg agtcgttaaa tcttttttat
     7681 gggtccttcc aggctttaag gaatattaat atggaaataa aggatcggtc tattacagca
     7741 attatcggtc catcaggctg tggcaaatct tctcttctta gactgtttaa tcgtatgaat
     7801 gacttaatac cgggagtcag ggttgagggt aaaataaatc ttgacggtgt ctccatatat
     7861 gataaaaaca tggatgttgt ggagcttaga caaaaagtgg gaatggtttt tcaaaagcct
     7921 aatacttttc ccatgtctat ttatgataat attatttttg gtccaaaaca ccttggacaa
     7981 aaaaacaaga aggaattaga tcaaatggtg gagcatagtt tacgcgaggt agctttatgg
     8041 gatgaagtaa aagacattct taataaatct gcttttgaac taggtgcagg tgagcaacag
     8101 cgcctgtgta ttgcccgtgc cctggctgtg aaacctgatg ttatattgat ggatgagtcc
     8161 accagcgctc ttgatcccga atctacactg aaaattgaag atttaatgca ggaactttct
     8221 aaaaattata ctattattat tgttactcac agtatggagc aagccgccag ggcatccgat
     8281 atgacggcat ttatgatgat ggcagatgac agggcaggaa cattggttga atatgccgaa
     8341 actgagcaac ttttttccaa tcctaaggat aaacggactg aggattatat aacaggtaga
     8401 ttcggataaa gaattatgga gactaattat gaattgtaaa ataagaaaac aaatgaaagc
     8461 aactgtaatg gcagcacaat tactcgtttt tatgatttta atatctgcct gcagtcgaaa
     8521 caacgaggta ggcgttattg ttgccggatc aacttccgta cagccttatg cggaggtttt
     8581 agctgaagaa tatatgatac ttaatccagg cactgaaatt gatattcaag gcggtggatc
     8641 ttctgccggc attactgctg caacaaacgg aacttcggat atagggatgt cctccagaga
     8701 attaaaggat aaagaaaaaa gcttgaattc tatagaaata gccaaggacg ggttagtagt
     8761 aatcgtaaat cctaaaaatc caatacaaga tttaacatta gatcaagttc gtgacatata
     8821 ttcagcctct atcactgaat ggaataagct tggaggaaca gactctaaga ttcatgtaat
     8881 aacccgcgag gaaggatcag ggacaagaag cgcttttgag gagctgctga tgggtgaatc
     8941 tgagataacc ccaaaagcca tagttcaaga ctccaatgga tcagtacggc tattagtagc
     9001 agatgaccca aattcaatag gttttatttc tatgggaata gttgatgaaa ctgtaaaagc
     9061 attaagcctt gaaggtgtta ctgcgaccag ggaaagtata ttagacggca gttacacttt
     9121 gtccagacca tttttgtttg taactttaaa cgaacccaca ggacaaacaa agcattttat
     9181 agattttgta ctttcggatg agggacaaaa attacttagt catgagggac ttgttccttc
     9241 aatgaataaa tgaaaggtac agtaagatga agaatattga tgaaaagaaa tatgagattt
     9301 tatttttaat tattgcgctt tcgtcactga ctgcattagc acttattacc ggatttatat
     9361 tttataaagg actgccaatt atagcaaagg taggtttatt tgattttatt tttggcatga
     9421 aatgggctcc cggaaagggt gaatttggta tttttcctat gattgtcggt tcagtatcag
     9481 taactttggg agcagcaatc atcggtgtgc caattgcaat ttgctgcagc atatttttgg
     9541 ctgagtttgc tcctgttacc atgagaaaca tatttagacc tgccatacag cttttagccg
     9601 gtataccttc agtagtatac ggattttggg gagttttgtt tattgttcct tttataagaa
     9661 actacctggg aggacctgga ctaagtatat tggccggttc cattattctt gcaataatga
     9721 ttttacccac tataataagc atcaccgagg tttctcttct tgccttgccc cgtcattata
     9781 aggagggagc acttgccctt ggacttaatc aatggcaaac aatctatgca ttactgctgc
     9841 ccgcagctaa gtccggtatc gtgtcgggaa taattcttgg tataggcaga gccattgggg
     9901 agacaatggc ggttattatg gttctgggta attctgtagc aattccggag tcaatccttg
     9961 accctgtaag aacattaact actaatatcg gtattgaaat gggatacgca tcaggcgaac
    10021 accagcaggc actttttgca acaggtattg tgctgtttgt aattataatg atattaaatg
    10081 cttctgccca atatatcact agaaagagat gaggttatta tgaaaattaa tccgcgaact
    10141 tcacaaaaaa tagcaaaatt tattatttgg ttggcagcaa ttcttgtttt agtagtactt
    10201 tttgcaataa ttacgtatat cttagcaaag ggcatacctc atttaagctt tggatttctg
    10261 actgaaattc cgcgaaatat ggggcgtgaa ggtggaattt cctctactat cgttggaaca
    10321 ctgctggtaa cagcattagc agtcattatt gctacaccat ttggaatcgg gacggcagta
    10381 tatctaaccg agtacacacg tgaaagcagg gtaacccgta taataaggtt cagcgcagag
    10441 tcactggcag gaataccatc catagtatac ggactttttg gatttatatt ttttgtaaca
    10501 tatctaaagt taggatggtc aattttatcc ggagggctga ctatggctat aatgattctt
    10561 cctaccataa tacgtacttc ggaggaagca attcgtacag tgccaaattt aaatcgtgag
    10621 gtgggatatt ctttgggagc cactaaatgg cagactatta ccagtaccgt gtttccatcg
    10681 gcactgcccg gcatagcaaa cggaataata ttaagtattg gccgctgtgt tgctgagact
    10741 gccgctgtta ttatgactgc cggttctgct ttgcgcatgc ccacatccat gttttcacca
    10801 acccgtacaa tggcagtgca tttttatata ttggccagag agggaatatc catggaaaat
    10861 gcctatagta cggcagccat tttaataatt ttgatatttt taattaatgt tgttttcaat
    10921 atgctggtca atagattcgt agctaagggc cgttaagagg tgaaaaataa aaattatgga
    10981 tcaacaacct aagataatta taaaagactt aaatttctac tataataaaa aacaagccat
    11041 taaaaactta agtctagata tatctaaaaa tgagatactg gcgatttttg gacctgcaaa
    11101 cagcggtata acaaccctgc ttcgcacatt aaaccgctta agcgacctga cttatggagc
    11161 gcggcatgag ggtgatgtac ttcttgacgg ggaaaatatt tatgctccgg atattaatgt
    11221 gactgagctt cgtcgcaggg tagggatggt atttgatgta ccggcgccac ttcccatgtc
    11281 catttttgat aatgtggcct ttgggcctcg catgagcagt ttaataaaca aaaaatctga
    11341 tttgtcagat aaggtggaac agtcattgcg catggctgca ctatgggatg atgtaaaaga
    11401 cagattggac actccagcaa tgagattgtc cgggggacag cagcaacggt tatgtattgc
    11461 aagagtttta gccctggaac cggaggttat tttactggac agaccatgtt cagcccttga
    11521 tcctgtatca acagcaaaaa tagaggattc tttaaaacgg cttagcgagc agtttacaat
    11581 agtaattgcc cctcataacg ttcaacaggc tactcgtatt gctgataatg ttgcgtttat
    11641 gttaatgggt gatttaatag aacatggcga tgcttttgag gttttatcaa atcctaaaga
    11701 caaacgtacc agtgattata ttaccggtag gtttggataa ggttattgaa tttttttaaa
    11761 tacttagcat atataactat ataaaatatg gaggtaggtt atgagaaata gatttgacaa
    11821 agagcttgag aatttaaaca atgaaattat tgaaatgggc actatgataa taagtgcaat
    11881 agaaaatagc gtctgtgcct taataaacca ggatatgtcc actgttaaag tagtaaatga
    11941 attagaaaat gaaatagaca gaatggaaaa ggttatagag tcacattgca tgagactgct
    12001 gatacaacaa cagcctgtgg catcagattt aagactgatt gaaactgcat tgaaaatgat
    12061 cacagacatg gagcgaattg gtgacaatgc agaagatata gcagagattg taaaatactt
    12121 cgaaaacaag aggtttatta aggatttaat ccatattccc aaaatggctg aagtgactat
    12181 aagcatggtt aaaagaagtg ttgaagcatt cgttaataag gacaaggatt tagctttaca
    12241 ggtttgttat gatgatgata tagttgatgg tttatttatt actataaaaa ccgaacttat
    12301 tgaaatgatt gggaaagatt ctaagaatgg ggaacaggct atagatttct taatgattgc
    12361 aaaatattta gaaagaattg gtgatcatgc ccaaaacatt gccgaatggg taatatttgc
    12421 aataaccgga gagcacaaaa acacagacga tatcggaaaa caataaaacg ataagggatt
    12481 ccgtggacag aactttaaaa aaaatttaaa aaatttttta aaataaatgt ttaaagtttt
    12541 ttttcagggg tatatatcta agaccacata aattttgtac cttgtcaact caatatggat
    12601 tactattaaa taatttgagg tttattgctt aagagataaa ccggctgcca tggtttttaa
    12661 gtgttgactg tgtaatatgc ttaagagaaa agtagttgat aattattttt aggaggatat
    12721 ttctaagaag tttcgaaaaa tagtttatgg tttaaaataa agtaacgttt atatatagaa
    12781 tcaaaaaatt atgtagagtt tttagtaaat aacaaacaaa aaataatatt agttatagat
    12841 ataccggtag tatgaaaaga aaaacatgga tagatcaggg gcagtaaggt tgcaaaagcg
    12901 atctttcaaa atcaggttat ttaaaaggaa atatattggg gaaaaagctt tgcagggaat
    12961 aaaattatgc tggtaaatca agaagaggag ccaataatgg gatttctcat cgaaaacagg
    13021 tagcacccga ttttcattga tcgcgaaaat tgggtgctat ctgatttttt gttttattca
    13081 ctttttcaaa ataaaatagc tccaaactaa aatttctctg tgccgaaggc gccttcccta
    13141 taatccttaa gtgcctggtc taattcttcc tttgtattca taacaaaagg tcccacaaga
    13201 acggcaggct cattaagggg ctggccgcta agcagtatta ttttagaatc ttttgacatt
    13261 gattttattt ctatttcgcc ttctttgttt tcaaagagaa caaagtcatt ttcactgcat
    13321 ggggtattgt taatatgtgg atctccttta ataacaaaaa gccccgtatt atagccggcg
    13381 ggttcattta aagtgacgct gccttcggct attaaatcaa ctatataaat attgacaggc
    13441 gaaaatgtaa gggcagggcc tttgacattt ttaaaatccc cggcaattac ggtaacttct
    13501 ccgaaatcat tgggaagttt tacttttccc atgctttcat ttgaaagctc ctgatatctt
    13561 ggagctgtat acttatcttt cttgggcaga ttaatccaaa gctgaatggc gtgaaacatt
    13621 ccgcctcttt gacgaaattc ttcctcatgg tattctttgt gcattattcc tgcgcctgcg
    13681 gtcatccact gaacatctcc cggatatata attccatgat tgcctgcatt atcgtgatgc
    13741 tcaacagcac cttgaaaaac aagggtaact gtttcaattc ccctgtgggg gtgaggacct
    13801 actcctttag gagattttga cgggggaaat attctgggag cattgtagtc cataagaata
    13861 aagggggaaa acctgtcaag gaggtttttg ctttcaggga agtaatttcg caccctgaag
    13921 gcggtgccaa cccaatgttc tttttctatt gaatatttgt cttcaattgg tctaaaattt
    13981 gacatattcc acctcaatct ttccgattta gttaagtgcc ttgtatttgt attactatta
    14041 tataaactgt tgaattatta tgcaagctct tgtttgacat ggatagggaa aaataatgtt
    14101 aaaatatcac ataatgtggt ataataaggt aaatattaat ttaaaaaagg agcattttat
    14161 ggatgattat ataaagtcaa actacataat agaagaaaaa ataatgatac aggaaattcc
    14221 ggcaattgtt tttaaaccta aagatgcggg ggagaaaatt cctacggcgg ttttttacca
    14281 tggctggagt tcaaataaag aaactcagcg catgagggga tttattttgg cctctgcggg
    14341 gtatcaggtg gttattcctg atgctgtata tcacggtgaa agaaacccct taaatgatta
    14401 tggccctgaa gcagccatag aatatttctg gtatgtggta tttaaaaatt tagaagaata
    14461 cagtgtcata gaaggagatc tggtaacaaa gtatggggct gaccccaaca ggattgcagt
    14521 aatgggtaat tccatgggcg gatttacttc cggtggtatt tttacacact acaagaaggt
    14581 aaaagctcta atagtgttta acggctgctg ctggtgggag gatttcaata agaaagcaga
    14641 tgttgcaatt accgagaaac tggaaaaggt tataaaaaag gttgaagaat tggacccaat
    14701 gaataattta aaccttctta ctgacagacc catactgctg ctccatggag acagcgacac
    14761 ttcggtgcct gttgaaagcc aaagagtttt ttatgataga ttaagtccca tgtacattaa
    14821 caaagaaaaa attaaactca tcgaatatcc caagttaaat catgttatta ccacaaacat
    14881 gatggaagaa agcataaatt ggctaggcag gtatatgtag aaatggatta attatagcgg
    14941 gatattaata atcgggggca gtatgtccaa acattcaaat cggatagtaa ttggatagct
    15001 aaaccgttac aatgagttac gggcgggctt tgataataaa agcccgcccg caaccttata
    15061 tattagaata aacaggaata attatcttat tccagtggtt ccaagcttgc ctggaaatga
    15121 cgaagtatct taggttccca ggtaatcctg tatcccttaa cgcttgctgc tctttcctta
    15181 attgctatga gagcttctgc cattatatca aaatgagctt gagtgtatac tcttcttgga
    15241 acggcaagtc ttgtaaattc aaaatctgct tccagctgct ctccggtatc aggattgttt
    15301 cctaacatat atgaaccgat gtcacaggtc cttattccgg cttctttgta taactcaatt
    15361 gccaaagcct gtgcagggaa ttcataataa ggaatatggg ggaacatatc ttttgcatct
    15421 atgaataatc cgtgtccgcc aaccggtgcc tggtatgcta tgcctgcatt atccaatttc
    15481 gcagctaaat attccaactg acctattctg tatcttaaaa agtcttcatc ctggccttca
    15541 taaaggccga ttgccattgc ctccaaatcc cttcctgata atccgccgta tgtagtaaag
    15601 ccttcaaatg aaatacagtt agcttttacc ttttcgtata gtgcctgatt atcttccttt
    15661 attcctatca agccgcccat gtttataatg gcatcttttt ttgcactcat tgtaaagaaa
    15721 tctgcatagc tgaacatttc tcttgttatg tcttttatag accagttttt gaattcttct
    15781 tctctctgct taacaaaata tgcattttca gagaatctgg cagcatcaat gtctaatgga
    15841 ataccgtatt ttttgcagac tgccgataca tctctcatgt tttgaactga tacaggctgt
    15901 cctcctgctg agttattggt tatagtcatt acaactactc ccacattctc actgcccagt
    15961 tcattaataa gcttttccag cttttcaacg tccatgttgc ctttaaatgg agctcttact
    16021 ttaggatcct tagcttcttc cacaacacag tcaattgctc ttgctccggc tatttcaaca
    16081 tgggctctgg ttgtgtcaaa aaacatattt gcaatagcat atttgccttt ggatactaaa
    16141 atcgggaaaa acaccttttc ggctgctctg ccttgatgta ccggctgaat gattttgtat
    16201 ccgaatatgt cctgcccagc ttttataaca ttgaaatagc tctttcctcc ggcatatgat
    16261 tcatcaccca ccattactcc tgcccactgg tcggaactca tagcatttgt tccgctgtct
    16321 gtcaataagt cgatgtaaac gtcttccgct ttgagattga acatattata atttgcctct
    16381 ttaatctttt gctccctctc ttcacgagtt aacatcttca gcggctctac cattttaatt
    16441 ctaaacggtt ctgcaacata cttaaaatcc atattatttc tcctcgtata atatgtttag
    16501 aactactttt taagtgttct aaacattaat tt
//
LOCUS       DAOPUT010000029        16518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3561_length_16518_cov_20.979165, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000029 DAOPUT010000000
VERSION     DAOPUT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16518
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3561_length_16518_cov_20.979165"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKK75_04160"
     CDS             <1..252
                     /locus_tag="PKK75_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82820.1"
                     /translation="ESFYISRLSSNRVKGALEALDKNPPGGEELKIFNDFLLKLISSG
                     IPYDEGKNVYNETVKQISNSNIKSILDEIFLERHWDTEY"
     gene            425..1132
                     /locus_tag="PKK75_04165"
     CDS             425..1132
                     /locus_tag="PKK75_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HNZ82821.1"
                     /translation="MKKFIIENNFWELFPDAKFGVVVCHSIDNSIIEEDKYLNMLRES
                     EKEALKYLDNPEFIQNDVVKVWREAFQKFKTKKGARCSIEALLKRVKNENQIGSINPL
                     VDIYNSISLKYALPCGGEDIDSFAGDNRLTKAAGNESFVTYGSDKSEPPYEGEIIYKD
                     DEGAICRCWNWRESVRTMLTENTKNAFLIIESVDEKRTEDLKKAINDLAKLVQENLGG
                     TCKTAILDINNKSVDLE"
     gene            1197..1877
                     /gene="sfsA"
                     /locus_tag="PKK75_04170"
     CDS             1197..1877
                     /gene="sfsA"
                     /locus_tag="PKK75_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HNZ82822.1"
                     /translation="MIYDNIKTAKFINRPNRFIANIYIDGKIEACHVKNTGRCKELLT
                     EGASIYVQELNNPNRKTKYDLISVYKGERLVNIDSQAPNRVFYEWLLEGKLFEKIKLI
                     KPECKYNNSRFDFYIEADNKKIFVEVKGVTLEEKGLVMFPDAPTERGLKHLNELSDSN
                     DEGYEAYVCFVIQMKDVLYFTPNYKTHEEFGETLKKISKKGVNLIAFDSQVTKNSITA
                     GDLVGIIL"
     gene            1977..3140
                     /locus_tag="PKK75_04175"
     CDS             1977..3140
                     /locus_tag="PKK75_04175"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNZ82823.1"
                     /translation="MKNKFIAKRYWKDQSTAMGQSDIIAKSYDDVINLSLGDPDLITH
                     DIIIDNSYADAKAGHTKYTDFRGDPELRQEIVNYYKEEYAMDVKDEEVFVCASACLGM
                     YLALEAIIDDGDEVILQAPYFTPYPQQVKLARGVPVELPTYEEEDYQINIKRLESFIT
                     ERTKALVINSPSNPTGSCLTVEVMKEIADIVLKYDLIVISDDIYTSFSYQNPFVPIAS
                     LPGMKERTIIINSFSKNFTMTGWRVGNIIAPDYIIKVIQQINENVVFTAPSISQRAAI
                     YALRNRREIQPPMVDEYRKRMFYAAERINRIPKLSVIYPPKGSFYLFINIKKTGLSSV
                     EAAELILKEAHVLVLPGDAFGNCGEGYVRIACTVNVEKLKEAFDRIEKITIFK"
     gene            3274..>3935
                     /locus_tag="PKK75_04180"
     CDS             3274..>3935
                     /locus_tag="PKK75_04180"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HNZ82824.1"
                     /translation="MESINEAYGINAVINNNIKGELPGFLSEEQVKGVRKFHSTIEGY
                     EPTPLVSLECLAEKLGISRIYVKDESKRFGLNAFKGLGATYAIARLMCEKLDVDIETI
                     DYNYFKLREVKEKIKDMVFVTATDGNHGRAVAWAAAQLGCKSIVYMPKGSSLKRLQHI
                     KDTGAEAFITDVNYDDAVRLARRKAEETGGYLVQDTAWEGYEKVPNWITQGYTTMAAE
                     AI"
     assembly_gap    3936..4035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4036..4335
                     /locus_tag="PKK75_04185"
     CDS             <4036..4335
                     /locus_tag="PKK75_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HNZ82825.1"
                     /translation="TVTWPILRDFAYAYASCPDYAAAKGMRILAAPMGSDKKVISGES
                     GAVGLGLLALILEKGELKNLKNKLKLNEKSVIICFSTEGDTDPEQYQAIISQPEN"
     gene            4602..5987
                     /gene="argH"
                     /locus_tag="PKK75_04190"
     CDS             4602..5987
                     /gene="argH"
                     /locus_tag="PKK75_04190"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNZ82826.1"
                     /translation="MKNLWGGRFSKTMEDYTADYNESISFDNILYKHSIKGSKAHVKM
                     LAEQQIISQKICEEILEGLSKVEKKIENNEVELNILDEDIMMIIEKELTKEIGESGKY
                     LHTARSRNDQNALDETLFLRDAVRNSINHLLDLLNVLVIKAEEESHKIMPGFTHLQHA
                     QPITAGFYYMAHFQGLRRNVDRFKELLNRIDKNPLGACAMAGTTLPIDRFITSKLLDF
                     SEPTENALDTVGTRDNIAEYLFNAALCMTHLSGFAEEIIVFNSQEFNFITIDDSFCTG
                     SSIMPQKKNPDIAELVRGKSGRAIGNLMTLLTVLKGTFLTLNKDYQEDKEALFDTIHT
                     LDRSLYIFARMLENITFNEGVLREQLKKGFIEATDVAEFLVKHDIPFREAHEIVGKLV
                     KYCELNKKTFPDINKEDLKEIGFPVELEDLSQFSIENCIKNRNSYGGTSYGEVNRQIK
                     NAKKYITDLKG"
     gene            5991..6365
                     /locus_tag="PKK75_04195"
     CDS             5991..6365
                     /locus_tag="PKK75_04195"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNZ82827.1"
                     /translation="MVKIEPRYNSENKGHYTPGIISNGILYISGQLSIDPDTRQVPDG
                     GIEAHAKLALKNLERVVIEAGLSKNNIIQCRVYVSDIDNWDAVNRVYAEFFGEHKPAR
                     IVVPVGKLHFGCLVEVEAIAEI"
     gene            6428..7186
                     /locus_tag="PKK75_04200"
     CDS             6428..7186
                     /locus_tag="PKK75_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNZ82828.1"
                     /translation="MEAIFRKKYTVHVSDIDFKRNLKLSSLFIYLQDMATEHAYNIGV
                     GRDALQAKYGIIWVLNRAAVEIIRYPVFKEEIAIETWPEQPDKVQFNRNFLIYDKDEN
                     IIAKAFSQWVVIDYNTRKLRRSSIIEEEFPKVDRERPLTLSDDKIKPIGNLDLIYEKT
                     VGYSDIDINEHLNNAKYIDYIMDCFSINLHKKYNISSIQIDYLHEALPGETIKLYTDT
                     AGVKDNYAYIEGINENTSSTIFKARITFLQEGNR"
     gene            7340..8113
                     /locus_tag="PKK75_04205"
     CDS             7340..8113
                     /locus_tag="PKK75_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNZ82829.1"
                     /translation="MISRKSVITFGMVAIPIAMYTATQDNDIHFNQLHADDSSRIRYK
                     KTCAHCGKEITSNEIVKGYEYDKDKYVVITDEEIEKIKTEKEKSIQILHFAQLNQISP
                     VYYEKTYQAAPEAGGEKAFELLRSALMAEQKVAIGKTVMGTKDTLMAIIPREEGILIS
                     TMFYADEVKEIQKQYTKPQVAAQELDMAKLLINSMDTPFDPARYKDEYQARLRELIEA
                     KISGKEVVAAEPESAGKVIDLMEALKASVEKAKKNKETA"
     gene            8115..10574
                     /gene="ligD"
                     /locus_tag="PKK75_04210"
     CDS             8115..10574
                     /gene="ligD"
                     /locus_tag="PKK75_04210"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HNZ82830.1"
                     /translation="MTAGLNEYNLKRNFEKTIEPEGKQKYSDESLRFVVQHHLAGRDH
                     YDLRLEWKGVLLSWAVPKGPSFNSKDKRLAVKVEDHPVEYRNFEGTIPKGEYGGGVVM
                     LWDEGYWEPYVNVEEGLSEGMLKFALKGSRLRGKWALVRIKGKGGETSDNWLWLKEKD
                     EYVRDDEVISEFTTSIRTGRTMDEIEKGQGEKLIKNPFQKADVQLAKLVRTVPEDNEL
                     LYEMKYDGYRVLAFVEGNSVRLITRNGNDITNRFYSIASSLIDLAQGRPMVLDGETVV
                     TDESGKTDFQALQNYLKNQGTQTLTYIVFDLLALDGQDMRGQTLVERKKKLENLLKDA
                     PINLYYSRHIKGQGKESFAAACEAGMEGIVGKKPDSVYSGTRNEDWIKLKCDKRQEFV
                     VGGYSLSDKRTRGISSLLLGLYAGDELIYAGRAGTGISENDMKVLESKFEGLKRKDPP
                     FKQVPKIKSNERIIWLEPELAAEIKFAEWTKDNLLRQASYKGLREDKNTKEIKRENQE
                     DDKQPEMNADNYLVRENHKQAKAITEKGLIIEGVKISSPDKIIYDELGITKEEVVRYY
                     SNAADRMLPYVRHKLLSIVRCPKGVSKACFYKKHPGPGTKGVITIPVITGNNETEEYF
                     YIENKTGLISEAQMGTLEFHTWAASIENLEQPDLLVFDLDPDEGMDLGRVRQGVEEVK
                     SILDELQLKSYLKTSGGKGYHVLVPLKPSADWEKFYDFAKQVAVVMENKWPERYTSNV
                     RKASRKGRIFIDWIRNARGATSIAPYSLRARKGAKVSMPISWDELYTVAPDSINMEEA
                     ILRIEGKDPWEDFFKNSQAIS"
     gene            complement(10614..11537)
                     /locus_tag="PKK75_04215"
     CDS             complement(10614..11537)
                     /locus_tag="PKK75_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNZ82831.1"
                     /translation="MAIRPPILRAGDTVGIVTLGSPPDAGTIDVGIQTLQSMGFNVLV
                     GDHAYSNAGIVAASPIDRASDLMNMFEAPDVRAIIPTRGGTGVSDIIPYLDYDVIRSN
                     PKIITGYSDITILLNVLYQLADLITFHSLLLLNFRPGTPAYNFNQFFTATSTITAPRP
                     LENPPEMPLISRVQGNVTGTIVGGNLASFVDSLGTPFEVDTTGRILFIEETHEAASRV
                     YRMIAHLIHAGKFRDCAGIIMGECSGCYANYGKSYEDIINEVIVPLGKPLMTNLASGH
                     GVYKMAVPIGAVTNLNTYSNTLTVLEPTVSL"
     gene            complement(11804..13174)
                     /locus_tag="PKK75_04220"
     CDS             complement(11804..13174)
                     /locus_tag="PKK75_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ82832.1"
                     /translation="MINNVNLTEGKIRSALVRLALPIMGTSFIQMAYNLTDIMWLGRL
                     STNAVAAAGAAGFFLWVGSALVMIAQVGVGVKVAHSYGKGDLEAAKTYINNGLQLNIS
                     IALLYSLFLYLFRHEIIGFFNIEDSSVVKMALEYLTIISIGMTFHFLNPVFSAILNST
                     GNSITPFKTNTIGLIANIIIDPLLIFGFGPFPALGIKGAALATIMSQFFVTIIFLIIG
                     KKYDTIYSHVKLFNQPNLQIIKDIVKIGFPPFVQVGIHAGISIVLTRILAVFGPVAIA
                     VQSIGSQIESITWMTTEGFSQAISAFTGQNYGAGKIKRIKEGYVKGIQIIGGIGVFAT
                     LLLIFGAELLFEIFTPNDPTAIKEGIIYLRILGLSQFLMAIEIGTTGAFNGLGKTIYP
                     TVNGIILNVSRIPAALILSKTALGLSGVWWAITISSNLKGLVLFVLFYFYWKHFEKNH
                     SKNNPL"
     gene            complement(13289..16450)
                     /locus_tag="PKK75_04225"
     CDS             complement(13289..16450)
                     /locus_tag="PKK75_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ82833.1"
                     /translation="MKTKVRALSFCLAVLTVLTMIPNLAFSASTISGQLIYTSADEWA
                     KPEIEKAFSAGIVPSRLFAADAKNPATREELCEIAVLLYEKVTKSEIEAANPNPFTDT
                     KNVEILKAYNLGITSGTSAANFSPSDITNREQVATMFGRTIRLMFPYDDYSTEGAPVF
                     LDQGYISSWALDHVKFMSKVGIIQGSNNYFMPKAITNDQIVSGYGTTKREEALAIAVR
                     IYNKYINHTASDSADITSKENFAKSLIRTNIPKLSESEKFSQIDFDNRMFRPIYKPKL
                     TLTAQDGYASDGTPNYSPSPYAPLVSSRGDKIESFKISLPSDVLSKTSSIIWQVSLVP
                     FDGRPVDFGSPAPGGLLMNGKLSSSATSFSIDFGKVEEADNALRSPKTNRIIFKPRLS
                     SKTIEMAGLTLPIAPINPNLISPINPNIIDPIIIDPDIINLPDIIVPKVLDASKLIPA
                     SPSEQKPVLQRTYYVRAYPADSFGNSIGDTGAGLPVIYGDPLPAKSDGTKKVYLPIYL
                     YMLQHSGPRRIERGEFPDNFLDINDGIMLNNSSIKTYSVLPLGYPSNTQELKVQVSLI
                     KYTGSADSNWNNTPGLVYERSIFPDDPEYLRLTDSDTQGIVIDFSKFIPPDDQLPEDK
                     NIPYYVRVVSFIKGTQPGSVKALSSETVAISYGKHNPQSDVVFLEQIKIEPPIPKITK
                     FSYTPIQWETTNWKYHYVVTKQPTYKEVFGSFMGSVMPDDGEALYPPYTVGTKLDFTP
                     PPPQVKSWWEEALDAITGFFQDLASFVSKVVNWVSKAYADLKSGLIKFVASAFPAGWR
                     GFIETALTAMVDYGLASIGIPPTLPNFDELSEMGIDYLATIAVEATGIPANELTEYGK
                     EKLKDELAGGIKENVASSAKTASPNPMNWNFVKLDPEELYKPAYMTVELYNPHNIATP
                     KGMLYCTVDTLLDLSLVETDQNVKTLYTFYGSPLIYLYKPVMGMKIPSIQPGEKLTVP
                     IILEEMVGNSFYSGGPPVKKNDFSTMYALGEYNFSITISYELPPLSEEIARQKYTQNA
                     IYSYSTLGNAISFTQMPNKSYNK"
BASE COUNT         5502 a         2757 c         3480 g         4679 t
ORIGIN      
        1 gaaagcttct atatatcaag gctgtcatca aatagagtta aaggggcact tgaggcactg
       61 gataaaaatc ctccaggggg tgaggaatta aagatattta atgacttttt gctaaaatta
      121 atcagcagtg gtattccata tgatgaagga aagaatgttt ataatgaaac agtaaagcaa
      181 atttcaaaca gcaatataaa gtcaattcta gatgaaatat ttcttgaaag gcactgggac
      241 acagagtatt aatcattcct tactttacat aattaatatt atgtaaagca aggaattttt
      301 ttagtttatt taaaatgata tacataatta atattatgta aaacagggaa tttctttgcc
      361 tattaataaa atacatgtgg tataataggt attaaagtaa gaaaaaatgt acaggagatg
      421 tcaaatgaaa aagtttatta ttgaaaataa tttttgggag ttgtttcctg atgctaaatt
      481 tggagttgtt gtctgccaca gcatagacaa ctctataata gaagaggata aatacttgaa
      541 tatgctgcgg gagtcggaaa aggaagcctt gaaatatttg gataatccgg aatttataca
      601 aaacgatgtt gtaaaggtat ggagagaagc ctttcaaaaa ttcaaaacaa agaaaggagc
      661 cagatgctcc attgaagcat tgttaaagag agttaaaaat gaaaatcaaa taggttctat
      721 aaatcctttg gttgatattt ataattccat ttcattaaaa tatgcattgc cctgcggagg
      781 tgaagatatt gattcatttg caggcgataa ccggctgaca aaggcagctg ggaatgagag
      841 ctttgttaca tacggctcag ataagagcga gccgccttac gaaggggaaa taatatataa
      901 agacgatgag ggggcaatct gcagatgctg gaactggcgg gaatcagtaa gaaccatgct
      961 tacagaaaac acaaaaaatg cttttttaat cattgaatcg gttgatgaaa aaagaacaga
     1021 agatttaaaa aaggctataa atgatttagc aaaattagtt caggaaaatt taggcgggac
     1081 atgcaaaaca gcaatcctcg atatcaacaa taaatcggtt gatttagaat aacttttgta
     1141 acgtgcaatc ctgagtaaaa cgaaggatat cacgtctatt ttgataagga gtaattttga
     1201 tttacgataa tataaaaaca gctaagttta ttaacagacc aaacagattt attgcaaata
     1261 tttatataga tgggaaaatt gaagcatgcc atgtaaaaaa tacaggacgc tgcaaggaat
     1321 tattaactga aggggcttca atatatgtac aagaattaaa taatccaaac cggaaaacaa
     1381 agtacgattt aatatccgtt tataagggtg aaaggttggt caatattgat agtcaggcac
     1441 ctaacagggt tttttatgaa tggttattag agggaaaatt gtttgaaaaa attaaattaa
     1501 taaaaccgga atgcaaatat aataattcac gttttgattt ttatattgaa gcagataaca
     1561 aaaaaatatt tgttgaggta aaaggggtta cattggagga aaagggcctt gttatgtttc
     1621 ccgacgctcc gacagaaaga ggactaaagc atttaaacga actttcagac agtaatgatg
     1681 agggctatga ggcttatgta tgttttgtta ttcagatgaa ggatgtattg tattttacac
     1741 ctaattacaa aacccatgag gaatttggcg aaacattgaa aaaaataagt aaaaagggtg
     1801 tcaatttaat tgcctttgac agccaagtaa caaaaaacag cattacagcc ggtgatcttg
     1861 taggtattat tctataagcc tattttacta ttaaatttag aataatttat atactataaa
     1921 cccgttactt tacataattt tatttatgta aatcagagac ttttggaggg aagaaagtga
     1981 aaaataagtt tattgcaaaa aggtactgga aagaccaaag cacagccatg ggtcaatcgg
     2041 atataattgc gaaatcatat gatgatgtaa taaatttgag tttgggcgac ccggacttaa
     2101 taacacacga tattattata gataattctt atgctgacgc taaggcaggt catacaaaat
     2161 acacagactt tcgtggagac cctgaattac gtcaggaaat tgttaattat tacaaagaag
     2221 aatacgccat ggatgtaaag gatgaggagg tctttgtttg tgcatctgca tgccttggaa
     2281 tgtacctggc cttagaagca ataatcgatg atggggatga agtaatattg caggcaccgt
     2341 actttacccc atatccccag caggtaaaat tagctagggg ggttcccgtt gaactgccta
     2401 cttacgagga agaagattat caaataaata taaaaagatt ggaaagtttt attacagaaa
     2461 gaacaaaggc attggttatt aactcaccaa gcaatcccac aggttcttgt cttactgttg
     2521 aggttatgaa ggagattgca gatattgtcc ttaaatacga tttgattgta atttcagatg
     2581 atatttatac atctttcagc tatcaaaacc cgtttgtacc cattgcaagt cttcccggta
     2641 tgaaggaaag aacaattatt attaattctt tctcgaaaaa ttttaccatg accggatgga
     2701 gagtaggaaa tattattgca ccggactaca taatcaaagt tattcagcaa attaacgaaa
     2761 atgtagtttt cacggcacca tcgatttctc aaagggctgc aatttatgct ttaagaaacc
     2821 gccgcgaaat tcagcctccc atggttgatg agtacaggaa gagaatgttt tacgcagctg
     2881 aaagaattaa tcggattcca aagctttctg ttatctatcc gccaaaggga agcttctatc
     2941 tgtttataaa cattaaaaaa accggtctgt caagtgttga ggcggctgaa ctaatattga
     3001 aggaagctca tgtattggtg cttcccggag atgctttcgg gaactgtgga gaaggctatg
     3061 ttcgtattgc atgtactgtc aatgtggaaa agctgaaaga ggcatttgac agaattgaaa
     3121 agattacgat atttaaataa ttaaaggagt ttatagatgg gtagcaaaat ggcttcccat
     3181 ttatgttgtt tttggcagga agtattgacc ttaataccct aattattata tagttatata
     3241 aaagttgtca caattaatga tgggggtatg taaatggaaa gtataaatga agcatatgga
     3301 atcaatgcag tgataaacaa taatataaaa ggtgagttac caggcttttt aagtgaggaa
     3361 caagtaaaag gcgtaaggaa attccattcc actattgaag gctacgagcc cacgccgctg
     3421 gtaagcttgg aatgcctggc tgaaaaattg ggcatatcca gaatatatgt aaaggatgaa
     3481 tcaaaaaggt ttggactcaa tgccttcaag ggcttggggg caacttatgc catagcaagg
     3541 ctcatgtgtg agaaattaga tgtggatata gaaacaatag attataacta ctttaagctt
     3601 cgggaagtta aagaaaaaat taaagacatg gtgtttgtca cagcaaccga cggcaatcat
     3661 ggaagagccg tggcttgggc tgcagcacaa ttaggctgca aatccattgt atatatgccg
     3721 aaaggttctt ctttgaaaag attacagcat attaaagata ccggtgcaga agcttttatt
     3781 acagatgtaa actatgatga tgctgtaaga ctggcaagaa ggaaggctga agaaacagga
     3841 ggatatttgg ttcaggatac agcctgggaa ggatacgaaa aagttccaaa ctggattaca
     3901 caaggttata ccaccatggc agccgaagcc atagannnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnacggt tacctggcct attttaaggg attttgcata tgcatatgca
     4081 agctgtcctg attatgcggc ggctaaaggg atgaggatac tggcagctcc catgggcagt
     4141 gacaagaaag tcatatctgg tgaatcgggt gcagtgggat taggattgct ggctttgatt
     4201 cttgaaaaag gtgaattgaa aaatctaaag aacaagctga aattaaatga aaaatcagta
     4261 ataatatgtt tcagtacaga aggagacact gaccctgaac agtaccaggc aattatttca
     4321 cagcctgaaa attaacggct tcaccatagc taatcaccac tgataaagga gaaggtgtcc
     4381 atgaccaaga tacagctcat gaaaatccag acagtgcgga gaataaataa agaataaaaa
     4441 tcctgtaaga gtataataaa gaatttttta aatgttttaa ataaaagggc agaaagccct
     4501 ttttcttatg gattctttga ataaattctg ctataatatg ccgtaagaaa tctttaaata
     4561 aaaaaattta tgattatata tgagtgaaag gatgaagtta tatgaagaac ttatggggcg
     4621 ggcgtttttc taagaccatg gaagattata ctgccgacta caatgaatca ataagctttg
     4681 ataatattct ctacaaacac agtataaagg gcagcaaggc ccatgtaaaa atgcttgctg
     4741 aacaacaaat tataagtcaa aagatatgcg aagaaatact tgagggctta tcgaaagttg
     4801 aaaagaaaat agaaaacaat gaggttgaat taaatattct tgatgaagat ataatgatga
     4861 ttattgaaaa agagctgact aaggaaatcg gagaatcagg aaaatacctg cacaccgcaa
     4921 ggagcagaaa cgaccagaat gcattggatg aaacattgtt tttaagggat gcagttagaa
     4981 attcaatcaa tcacctatta gatctgctta atgtcctggt aataaaggca gaggaagaat
     5041 cccataaaat aatgccggga tttacacatt tgcagcatgc tcagcctatt actgccggat
     5101 tctactatat ggctcatttt caaggactca gaaggaatgt tgacaggttt aaggaactgt
     5161 tgaatcgaat agacaaaaac cccttgggcg cttgtgcgat ggcaggaacc acactgccca
     5221 tagaccggtt tataacatca aaattgctgg atttttccga gcctacggag aatgcactgg
     5281 acacggtggg cacaagggac aacattgcgg aatacttgtt taatgctgcc ttgtgcatga
     5341 ctcatttaag cggttttgca gaagaaatta tagtttttaa ttctcaagag tttaatttca
     5401 tcactattga tgacagcttt tgtacaggaa gcagtattat gccgcagaag aaaaatccgg
     5461 acattgcaga attggtaaga ggcaaatcag ggagggccat aggcaatctc atgacacttt
     5521 tgactgtttt aaagggtaca tttttaacat taaacaagga ttatcaggaa gacaaggaag
     5581 cactttttga cactatacat accttggaca gaagcttata tatatttgca aggatgcttg
     5641 aaaacataac cttcaatgag ggtgttttaa gagaacagct gaaaaaaggc tttatagaag
     5701 caactgatgt tgcagaattc ctggttaagc atgatatacc attccgggag gctcatgaaa
     5761 ttgtagggaa attagttaag tattgcgagc taaataaaaa gacatttccg gacattaaca
     5821 aagaagattt aaaagaaata ggcttccctg ttgaacttga agacttaagt caattttcta
     5881 tagaaaactg cattaagaac agaaacagct atgggggcac atcatacggt gaggttaaca
     5941 ggcaaattaa gaatgccaaa aaatatataa ctgatttgaa aggataagat atggttaaaa
     6001 ttgagccaag atataattca gaaaataaag gtcattatac gccaggtata atttcaaatg
     6061 gtatcctgta tatatcaggt cagttgtcaa tagaccctga tacccggcag gtgcctgatg
     6121 gaggaattga agcacatgca aagctagctt tgaaaaattt ggagagagtt gtaatagaag
     6181 ccggcttatc caagaataat attattcagt gtagggtata tgtatccgat attgataatt
     6241 gggatgcggt aaatcgtgtt tatgccgagt tttttggtga gcataaacct gcccgtattg
     6301 ttgtgccggt gggaaaactg cactttggat gtcttgttga agttgaggct attgcagaga
     6361 tataagacag taaggaatta ccccttttat ccgatgcgga tgagagggtt gtaaatggag
     6421 gtgcttaatg gaagcaattt ttagaaagaa atatacagtt catgtaagcg atattgactt
     6481 taagcggaat cttaaactta gctccttgtt tatttactta caggatatgg cgacggaaca
     6541 tgcctataat atcggagtgg gaagggatgc attgcaggca aaatacggaa taatatgggt
     6601 tttaaaccgg gcagcagttg aaataataag atatcccgtt ttcaaggaag aaatagcaat
     6661 tgaaacttgg ccggaacaac ctgacaaggt gcagttcaac aggaattttt taatctatga
     6721 caaggatgaa aatataatag caaaggcatt cagccaatgg gtggtaatag attacaacac
     6781 taggaaactt agaagaagca gcataataga agaagaattt ccaaaggttg acagagaaag
     6841 acctcttacc ctttcagatg ataaaatcaa gccgataggc aatcttgatt taatctatga
     6901 aaaaaccgtt ggctacagtg atatagatat taacgaacat ttgaacaatg ccaaatacat
     6961 tgattatatt atggactgtt tcagcataaa ccttcacaaa aaatataata tcagctcaat
     7021 tcaaattgat tatctgcatg aagccctgcc cggtgaaacc attaagcttt atacggatac
     7081 tgccggtgtg aaagataatt atgcctacat agaaggaata aacgaaaata caagttcaac
     7141 aattttcaag gcgcgaatca ctttccttca agaaggaaat aggtaaaaac cctgcctgca
     7201 gattttaaag agcagttgac aagagaaaga catggtgact tttcacctgt ttttttttgt
     7261 gaattcattc ttgctttatt ggcaatataa atcgaataca tagaccataa ttggcaacaa
     7321 taaccttgga ggtgattatg tgatatcacg taaatcagtt ataacttttg gcatggttgc
     7381 cattccgatt gccatgtaca cggcaaccca ggacaatgac attcacttta accagcttca
     7441 tgcagatgac agcagccgaa tcagatacaa gaaaacatgt gctcactgcg gcaaagaaat
     7501 tacttctaat gaaattgtaa aaggatatga atatgacaag gacaagtatg ttgtaattac
     7561 agatgaagaa attgaaaaaa taaagactga gaaagaaaaa tccattcaaa ttcttcactt
     7621 tgcccaactt aatcaaattt cccccgtcta ttacgaaaaa acctatcagg cggcccctga
     7681 ggcaggaggg gagaaagcct ttgaactgct gcggtctgcg ctgatggctg agcaaaaagt
     7741 agccattgga aagacggtaa tgggtaccaa ggatacattg atggctataa ttccccgtga
     7801 ggagggcatt cttatttcca ccatgtttta tgctgatgaa gtaaaggaaa ttcaaaagca
     7861 gtatacaaag ccccaagtag cggctcagga gcttgatatg gcaaagcttt tgattaattc
     7921 catggataca ccctttgacc cggccagata caaggatgaa taccaggcaa gactgcgtga
     7981 gctcattgaa gcaaaaatat cagggaagga agttgttgca gcggagcctg aaagcgccgg
     8041 caaggtaatc gaccttatgg aagctttgaa ggcaagtgtg gaaaaagcta aaaagaataa
     8101 ggaaacagca taatatgact gcaggattaa atgagtacaa tctaaaacga aatttcgaaa
     8161 aaacaataga gcctgaagga aaacagaaat attcagatga aagcttaaga tttgtagtac
     8221 agcaccactt agccggaaga gaccactacg atttacgtct ggaatggaaa ggagttctgt
     8281 taagctgggc ggttccaaaa ggtccttctt tcaatagcaa ggacaaaaga cttgcagtaa
     8341 aggttgagga ccaccctgtg gaatatagaa attttgaagg cacaattcca aagggtgaat
     8401 acggcggagg cgtggtaatg ctttgggatg aaggttactg ggagccctat gtgaatgtgg
     8461 aagagggatt aagtgaaggt atgcttaaat ttgctttgaa gggaagccgt ttaaggggaa
     8521 agtgggcttt ggtacgaatt aaaggcaagg ggggagaaac ttcggataat tggctgtggc
     8581 ttaaggaaaa ggatgagtat gtcagggatg atgaagtaat ttctgaattc acaaccagta
     8641 tcagaacagg acggacaatg gatgaaattg aaaaaggtca aggcgaaaaa ctcataaaaa
     8701 atcctttcca aaaggcagat gtgcagcttg ccaaattagt caggactgtt cccgaagata
     8761 acgagctgct ttatgaaatg aaatatgacg gctacagagt tttggcattt gtagaaggca
     8821 acagtgtccg gcttattaca agaaacggca atgatataac caatagattt tattcaatag
     8881 cttcttccct tatagacttg gctcaaggaa ggccaatggt tttagacggt gaaacagttg
     8941 ttaccgatga atcaggcaag actgattttc aggctctgca gaattattta aaaaaccagg
     9001 gcactcaaac tctgacttat attgtctttg atctgcttgc tcttgatgga caggatatga
     9061 gaggacaaac attggttgaa aggaaaaaaa agcttgagaa tttattgaag gatgcaccaa
     9121 taaatcttta ttacagccgt catataaagg gacaggggaa ggaaagcttt gctgcagcct
     9181 gtgaagcagg catggaaggc atagtgggga aaaagcctga ttctgtttat agcggaacaa
     9241 ggaatgaaga ctggataaag cttaaatgcg acaaaagaca ggaatttgtt gtcggagggt
     9301 attcactatc agacaaaaga acaaggggga taagctctct tcttcttggt ctgtatgcgg
     9361 gtgatgaatt aatatatgca ggacgtgcag gaactggaat aagcgaaaat gacatgaagg
     9421 ttcttgaaag caaatttgag ggtttaaagc gcaaagatcc tcccttcaag caggtgccga
     9481 agataaaatc aaatgaaagg ataatatggc tcgagccgga acttgcagca gaaattaaat
     9541 ttgccgaatg gacaaaggat aatctgttaa ggcaggcgag ctacaaagga ttgagagaag
     9601 ataagaacac taaagaaata aaaagagaaa atcaggagga tgataaacag ccggaaatga
     9661 atgcagataa ttatttagta agggaaaatc ataaacaagc aaaggcaata acggaaaaag
     9721 gtcttattat tgaaggagtt aagatttcaa gcccggataa aataatatac gatgaactgg
     9781 gtattacaaa ggaagaagtg gtcagatact attcaaatgc tgctgaccgt atgctgccct
     9841 atgtgagaca taagcttcta agtattgtcc gttgtcccaa aggagtgtca aaggcttgct
     9901 tttataaaaa acatccggga cccggcacca agggggttat aactatacct gtcattacag
     9961 gcaacaatga aacagaggaa tacttttata ttgaaaacaa aaccggtctt atatccgaag
    10021 cacagatggg cacattggaa tttcatacct gggcagcctc tattgaaaat cttgagcagc
    10081 cggacctcct ggtatttgat ttagaccctg atgaaggaat ggacttggga agggttcgcc
    10141 aaggtgtgga ggaagtgaaa agcattcttg atgaattgca gctcaaatca tatctaaaaa
    10201 ccagcggcgg caagggatac catgttcttg ttcctttaaa gccgtctgca gactgggaaa
    10261 aattttacga ttttgcaaag caggttgcgg ttgtaatgga aaataagtgg cctgaaagat
    10321 acacgagcaa tgtaagaaaa gccagtcgaa aaggcagaat atttattgac tggattcgaa
    10381 acgccagagg agccacaagc attgcaccct attcattaag agcaagaaaa ggagctaagg
    10441 tttcaatgcc gatttcatgg gatgagctgt atactgttgc tcctgacagc atcaatatgg
    10501 aagaagctat tttaaggatt gagggaaaag atccatggga ggattttttt aagaatagcc
    10561 aggcgatttc ctgatattca ttaatatggg taaccgtatc atcctgatgg aacctacagg
    10621 ctcactgtag gttccagtac tgttaatgta ttgctgtaag tattgagatt tgtaactgca
    10681 cctatgggaa cagccatttt gtatactcca tgaccggaag ccagattagt cataagtggc
    10741 ttgcctaaag gaacaatcac ttcatttatt atgtcctcat atgattttcc gtaatttgcg
    10801 tagcagcctg agcattctcc cataataatc cctgcacagt ccctgaattt tcctgcatga
    10861 atcaagtggg caatcattct gtagacacgg cttgctgctt catgagtttc ttcaataaat
    10921 aaaattctgc ctgttgtatc cacttcgaag ggagttccca agctgtcaac aaaggatgca
    10981 agatttcctc ccacaatggt tcccgttaca ttgccctgga ctctgcttat cagcggcatc
    11041 tcaggagggt tttccaaagg tcttggggca gttatggttg atgtggcggt aaaaaactga
    11101 ttaaagttat atgcaggagt tccaggtctg aaattcaata aaagcaggct gtgaaaggtg
    11161 attaaatctg ctaattgata aagaacattc aataaaattg ttatatcgct gtatcctgtg
    11221 atgatttttg ggttgcttct tattacatca tagtcaagat agggaattat atccgaaacc
    11281 cccgtgcctc ctcttgtagg tatgatagct ctgacatcgg gagcttcaaa catattcatc
    11341 aaatccgaag cacggtcaat gggggatgct gcaacaatac ctgcattgga ataggcatgg
    11401 tcgcctacaa gaacattaaa tcccatagac tggagggttt gtattcctac atcgattgtt
    11461 ccggcatccg gagggctgcc cagggttact atgccgactg tgtcgcctgc ccgcaaaata
    11521 ggcggtctga tagccatttt ttcctcctga taatatccat tatttaatta ttatatgaag
    11581 aaatatttta ttggttaata aattaggaaa ttctgatata tagtgaaatg tgcaaaattt
    11641 caagcagtac gttattattt cataaaatag tcttattttc atgtagaatt tgataaaaaa
    11701 cacaaaaaga atgtcaatgt ttgacttata ttacattatt gttaatgcac taatcaaaat
    11761 tttacacacg tgattctaaa atatttgtgg ggagattttg ttattataaa ggattattct
    11821 tggaatgatt tttttcgaaa tgcttccagt agaaataaaa taaaacaaac aacaccagtc
    11881 ctttcaaatt ggaggatatg gtaattgccc accaaacacc cgacaaacct aaggctgttt
    11941 tgcttaaaat caaggcagca ggaattcttg aaacattcaa tataattccg tttacagttg
    12001 ggtagatagt ctttcccaat ccgttaaatg ctccggtagt cccaatttca attgccataa
    12061 gaaattgaga taaacccaga atcctcagat agattattcc ctcttttatc gcagtaggat
    12121 cattgggagt gaaaatttca aataggagtt cagcaccgaa tattaacaaa agggttgcaa
    12181 atacacctat acccccgata atttgaatcc cttttacata tccttcctta attcttttta
    12241 tctttcctgc accgtaattt tgtcccgtaa atgcagaaat tgcttgagag aaaccctcag
    12301 tcgtcatcca tgtgatagat tcaatttgag agccgatact ttgaacggca attgccaccg
    12361 gcccgaagac tgccagtatc cttgtaagta ctatagaaat gcctgcatgg atgcctactt
    12421 gaacaaaggg aggaaaacct attttgacta tatctttaat aatttgaagg tttggttgat
    12481 taaataattt aacatgcgaa taaattgtgt catatttctt tcctataatc aagaagatta
    12541 tggttacgaa gaactgagac attattgttg ccaatgcagc acctttgatt cctaaggcag
    12601 ggaagggacc gaaaccgaat atcaaaaggg ggtcaatgat aatattggca attaatccga
    12661 tggtatttgt tttaaaagga gtaatgctgt ttcctgtgct gtttaaaatt gcagaaaaaa
    12721 caggatttaa aaagtgaaat gtcatgccta ttgatataat tgtcaaatat tcaagagcca
    12781 ttttaacaac acttgaatct tcaatattaa aaaatccgat tatttcatgt ctgaataaat
    12841 ataaaaataa agagtatagt aaagcgatgg atatattaag ctgaagtcca ttgttaatat
    12901 aggtttttgc tgcttccaaa tccccttttc cataagaatg ggcgacctta acacctactc
    12961 ctacctgagc aatcatgacc agggctgaac caacccacaa aaaaaaacct gccgcccctg
    13021 cagctgctac ggcattggta cttagcctgc caagccacat aatatctgtt aggttgtaag
    13081 ccatttgtat gaatgaagtt cccattatgg gaagtgccaa tcttaccaac gcgctgcgta
    13141 tttttccttc tgtcaaattt acattattta tcattcttat atccctaatt aagtctccca
    13201 attattttaa cccatccatt tgtattgtac aagcaaaata taataccccc attttttaga
    13261 tggaggtatt tattgcaaaa caaattatct atttattgta actcttatta ggcatttgag
    13321 tgaaacttat tgcattgccc aaagtgctgt aagagtagat ggcattttgc gtgtattttt
    13381 gccttgcaat ttcttcagat aaaggaggca gttcataact aatggttata ctgaagttat
    13441 attctcccaa ggcatacata gtgctgaaat cattcttttt cacaggcggt ccgccgctgt
    13501 agaatgagtt tccaaccatc tcctctaaaa ttataggaac agtaagtttt tcaccgggct
    13561 gaattgatgg aattttcata cccattaccg gcttataaag atagataagc ggtgagccat
    13621 aaaacgtata cagtgttttt acattttggt cggtttcaac aagggataaa tccagcaatg
    13681 tatcaacagt acaatacagc atacctttag gtgttgctat gttatgagga ttgtaaagct
    13741 ctacagtcat ataggctggt ttatataatt cttcaggatc aagtttaaca aaattccagt
    13801 tcattggatt aggcgaagca gtcttagctg agcttgcgac gttctccttt atccccccgg
    13861 ctaattcatc tttcagtttt tctttgccat attccgtcaa ttcattagca ggaattcctg
    13921 ttgcttccac agctatggtt gctaagtaat ctattcccat ttcactgagt tcgtcaaaat
    13981 taggcagagt tggcggaatt ccaattgatg caagaccata gtcaaccata gcagtaagtg
    14041 cagtttcaat aaatcctcgc cagcctgcag gaaatgcaga cgctacaaat ttaatcaagc
    14101 ctgatttcag gtctgcatag gctttgctta cccagtttac aaccttcgaa acaaatgaag
    14161 ctaagtcctg gaagaatcca gttatagcat ctaatgcttc ctcccaccag gattttacct
    14221 ggggaggtgg tggagtaaaa tcgagctttg tacccacagt atatggcgga taaagtgcct
    14281 caccatcatc gggcataact gatcccatga atgaaccaaa tacttccttg taagtaggct
    14341 gtttggttac cacatagtga tacttccagt ttgtagtttc ccattgtata ggagtataag
    14401 agaattttgt aattttgggt atcggcggct ctattttaat ctgctctaaa aatacaacat
    14461 ctgattgagg attgtgcttt ccataactta tggccacagt ttcagaagat aatgctttta
    14521 cggaaccggg ctgggtacct ttgataaaag aaacaacacg tacataataa ggtatatttt
    14581 tatcttccgg cagctggtca tcagggggaa taaattttga gaagtcaata actatccctt
    14641 gtgtatcgct gtctgttaac cttaagtatt cagggtcatc aggaaaaata ctcctttcat
    14701 aaacaagacc tggagtgttg ttccaattac tatctgctga acctgtgtat tttatcaaac
    14761 ttacctgaac cttgagttcc tgggtatttg aaggatatcc caaaggcagt acggaatagg
    14821 ttttgatact gctattgtta agcattatac catcattaat atctaagaaa ttatcaggga
    14881 actcacctcg ctcaattctt cgaggtcccg agtgctggag catataaaga taaatgggga
    14941 gatatacttt tttggtgccg tcactctttg caggaagggg gtcaccatat attacaggca
    15001 ggcctgcccc tgtatcccca atgctattac caaaactgtc agccggatat gcccttacat
    15061 aatatgtcct ctgcaaaaca ggcttttgtt ctgaaggaga ggcaggtatg agtttacttg
    15121 catccaatac ctttggcaca ataatatcag gcaggttaat aatatcaggg tcaataataa
    15181 tagggtcaat aatattgggg ttaatcgggc tgatgagatt tggattaatt ggtgcaatgg
    15241 gcagggtaag accagccatc tcaattgttt tggaactcaa tctcggctta aatataattc
    15301 tatttgtttt agggctgcgc agagcattgt ctgcttcttc aacctttcca aaatctattg
    15361 aaaaagaagt tgccgagctt gacagcttgc cgttcatcaa gaggccgccc ggagcaggcg
    15421 acccaaaatc aacaggtctg ccgtcgaaag gcactaagga aacctgccat attatggagc
    15481 ttgtttttga aagaacatcg cttggcagtg aaattttaaa tgattcaatt ttatcccccc
    15541 tgctggatac caatggtgca tagggtgacg gtgaataatt cggagtgccg tcggatgcat
    15601 aaccgtcttg ggctgtcagt gtaagttttg gcttataaat aggtctgaac atacgattat
    15661 caaaatcaat ttgtgaaaat ttttcactct ctgataattt tggaatattg gttctgatca
    15721 aggacttggc aaaattttct ttacttgtta tatcggcaga atcagatgct gtatgattta
    15781 tgtacttgtt gtaaatcctt actgctattg ctaaagcttc ttctctcttt gtggtaccgt
    15841 agcctgaaac tatctggtca tttgttatgg ctttgggcat gaaataatta tttgagcctt
    15901 gtattattcc gactttgctc atgaatttca catgatctaa agcccaggat gaaatatacc
    15961 cctggtctaa aaaaacaggt gccccttctg ttgaataatc atcataaggg aacattaaac
    16021 ggatggtacg gccaaacata gtagctacct gctccctgtt tgtaatgtca ctaggtgaaa
    16081 agtttgcggc agaagtacct gatgtaatgc ccagattgta tgctttaagt atttccacat
    16141 ttttcgtatc ggtaaaaggg tttggatttg cagcttcaat ttcacttttt gttacctttt
    16201 cataaagaag cacggcgatt tcacatagtt cctccctggt agcaggattt tttgcatcag
    16261 ctgcaaataa tcttgaaggg actattcctg cactaaaagc tttttcgatt tcaggctttg
    16321 cccattcatc agcagaagtg taaattaact gacctgatat agttgatgcc gagaaggcta
    16381 aattcggaat catggtaagc acagttaaaa ccgctaagca aaatgataag gctctgactt
    16441 ttgttttcat aaagcacctc ctttttttac tcaatagaat acatatatat tcctagttcc
    16501 atcttaatcc ttttaatt
//
LOCUS       DAOPUT010000030        16456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3581_length_16456_cov_16.759222, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000030 DAOPUT010000000
VERSION     DAOPUT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16456
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3581_length_16456_cov_16.759222"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1708
                     /locus_tag="PKK75_04230"
     CDS             326..1708
                     /locus_tag="PKK75_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNZ82834.1"
                     /translation="MDVFLYFGMLLALTLIIAAGIYSGKKVKVSSDFSVGSRKAGYMI
                     VAGTITGTLVGGASTVGTSELAFTYGFSAWWFTLGAGIGCLLLGLVFVESIYRNGNET
                     VSQILADEYGNKVGLVSSVFVSIGMFINIVAQILSATAIIVSVFNITSFTASCIAALL
                     MIIYVVFGGVWSTGIVGIVKLLLLYISVIIGGSMALKLGGGISHFIEVLPSEQYFNLF
                     ARGKSIDLGAGFSLIVGVLSTQTYIQAVLSGKDKSASQKGALLSAVLIPPIGLAGIFI
                     GMYMKLNYPDIAPSSTLPHFVLNYMPPFVSGIIMAALLVTVVGTGAGLALGISTVITK
                     DVYKNYIKRNSDELTLLNISRLLIVVVLAAAVIFTAGNMKSYILEWSFMSMGLRGAAV
                     FVPLCSAFFLKGRITSRFALAGVLLSPVSVLIGKTVMHTEIDSLFIGIAVNVIIFTTG
                     ILSNKKADIN"
     gene            1805..3712
                     /locus_tag="PKK75_04235"
     CDS             1805..3712
                     /locus_tag="PKK75_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ82835.1"
                     /translation="MQNRILLVSSNDELTKLAEKVSKNLNINYDLYEGGITKDGHIHA
                     KNNSHKYDVIISSGGTAEAIRKIVSKPVVSVEISAADILYAIYKAKKFKNKIGIVVYK
                     NDELAQFYKLKNFLNLDCEIFEYETKSEIINKMADATSLGRLTLVGIGGCIKETAIDH
                     NLDYVIIESSEKSIRDALINAANICELTKNDKERAKRYQAILDYSGEGIISYDKNNKV
                     TSINAAAEKILDVKYNDVFNKSIEAINDNFKVLLGDGNKLDNKLTKINGKQVLVNRIP
                     IIIDNDTLSVVVNIQETSNIQKIERNLRAELYRKGLVAKHTFSDIIGNSEIMKDTINK
                     AKRIGKTESTILITSETGCGKELFAHSMHNISSRNGGPFVAVNCAALPENLLESELFG
                     YEEGAFTGAKKGGKIGMFELAHKGTIFLDEISEIPLDLQGRLLRVLQEREIQRVGGDS
                     VVNVDIRIIVASNKNLFEKVINGSFREDLFFRINVLDLYIPPIRERKQDIPLLLDNCF
                     KTIGSRIRVNNFSREALNWIMKYDWKGNVREIENFAQRMFVLLEDCADINENSLENII
                     NYKLISSLSTKYSLENKDNIIQIEIGNIKEMESKIILEAAKKYSGDKTMLADKLGISR
                     TTLWKKLKEAD"
     gene            3986..5332
                     /locus_tag="PKK75_04240"
     CDS             3986..5332
                     /locus_tag="PKK75_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HNZ82836.1"
                     /translation="MVLVMAVLTYIIPAGEFVRVEDAATGRTVIDPTSYHAVDQNPTT
                     FFELWKAVPRGMVDAASIIFFMFIISGSFGVISSTGAIEAFISRVALMLKGKDKIMVP
                     MIVFIFSIFGGTIGMAEETIIFIPLGIVLARALGYDAIVGTSMILLGCACGFTSGFMN
                     SFTVGVAQGLAQLPLFSGIGLRIVIWFVFVIFTSVFILRYGAKIKADPSKSMVYELEV
                     AEKDTIVDLSNLTEMKREHYFVMITMIIGFIAIIFGVFKEGWYITEISAAFLAMGVIS
                     GLLGKMGPSKIAQQFIIGAKDIVFGALVVGIARGILMVMKDGVIIDTIVNTLAMIIQT
                     LPRSISVLGMYIVQVILSIIIPSGSGLAATTMPIMVPLSDVLEINRQVAVLTYQFGDG
                     ISNSFIPTAASCMGALSVAKISYEKWLKWSVPLIIGWLILGGIFCMIGMAINYGPF"
     gene            5389..6576
                     /locus_tag="PKK75_04245"
     CDS             5389..6576
                     /locus_tag="PKK75_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNZ82837.1"
                     /translation="MMEYTESMNLTEAVKLTQELVRIPSHKDVEDRESAVAEFIYNYC
                     KENGLEVEYQVVEGKRRNVIARLRGNGTGKNLMFSGHIDTVPPYDMEYDDPYCADIVD
                     GYLLGRGCNDMKGAVACMITAMVNIKNKGKVLNGDIILAAVVGEEEKSDGTAFVVKSG
                     ITADGAIEGEPSNYGYSLGHRGLEWIEIKFIGKRAHGCTPELGINAISKASKFIQKIE
                     TELMPKLKERKNEWMGPDIMNFGYIHGGAAPSFVADNCVLQFDRRYTPGETVDDVIKD
                     YQDIIDELKAEDSQFNAEIIRMEENLMDDFDHAPLIAEPDSQIAKTVYKAIKEFIGKE
                     PEIVQRGWTDAGLLSTYGKIPTVVTGPGNVKWSHAKDEKIPVADLVNYVEIYTNIAEQ
                     FCI"
     gene            6771..6959
                     /locus_tag="PKK75_04250"
     CDS             6771..6959
                     /locus_tag="PKK75_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HNZ82838.1"
                     /translation="MQGKVQKMDHHNPGIKCVVNTCYYYSDGDHCNAQKIEVQKRDAS
                     TSKETDCATFTNNHQSMS"
     gene            7253..7897
                     /locus_tag="PKK75_04255"
     CDS             7253..7897
                     /locus_tag="PKK75_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNZ82839.1"
                     /translation="MKKITKIEYQKKNKERLNIYIDDEYGFSVDISILIEYSLKKDMV
                     LDDSLIDAILRSEERTSVYNYGISVLSRYARSEYELRLKMKNKGFEPELIDKAVGTLK
                     EQKYLDDDRYCEMFINDKINISKDGVRKIKEALYYKGIDKKIIEEKIKNISQDSEEER
                     ALILGSKKLPNIKETDTRKKIAKLSNYLIGKGFEYETVNKTVRKLLSIEADDFY"
     gene            7910..8797
                     /locus_tag="PKK75_04260"
     CDS             7910..8797
                     /locus_tag="PKK75_04260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNZ82840.1"
                     /translation="MKEFAEYISDAFVHCRMICYEGKFDIVAIYANKQLEQIASRTLD
                     QLIGRKMTDVFPNLSDSIFDWPKILCEAAMTSEHTVIEQYVNAFEKFVRFNVFGFKDD
                     TFYVTIQDLTNKREIRRIILEKDRQIEHLENEIKSRASIEPLTNLYNFQFITDCIKQS
                     IQCYKEEGTVFCIFLIDIDNFKAFNLKYGMETGDMVLQDIAHILSLSARKIDVVGRYG
                     NDKFIMILNNVELDIAKIIIEKFKMEIGRYDLNINKSNIYVSGAVVEYRGESLEEFIE
                     KAEKLLSKAQSMGKGKILS"
     gene            complement(8823..9992)
                     /locus_tag="PKK75_04265"
     CDS             complement(8823..9992)
                     /locus_tag="PKK75_04265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNZ82841.1"
                     /translation="MRNSADINKVCPVIKGQLSSSSNISIPVIISYKSSKKIKEGEIS
                     SLSGKLKHSLPIINGCACEMNIDSILRLTSDPDVEFISYDSKVFVVMEEARKTIGADY
                     SLNTKYTGKGVTVAIIDTGIAPHADLIYPAKRIVGFKDFVNNRSNYYDDNGHGTHCAG
                     ILAGSGYSSKGRYKGIAPEANILSIKVLDENGNGNTSDILSTVQWIIESKEVYKTKII
                     NFSLGAIAQYKERRDPLVKAANRAIEEDLIVVAAVGNSGPMHNTILSPATSRYVISVG
                     SVNDRGTSYPKNETIAEFSSRGPTLDRVRKPDLIAPGVDIISLSSKNLNGYTTLSGTS
                     MSAPMISGVCALLLNENPDYSHFDIKRILLNSCSKIKASSYEQGAGVLDMRRIFS"
     gene            10209..10892
                     /locus_tag="PKK75_04270"
     CDS             10209..10892
                     /locus_tag="PKK75_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ82842.1"
                     /translation="MPKILVVDDERPIAEIIKYNLQKEGYEVQTAYDGDEAIKAVHQM
                     EPELIILDIMLPKKNGFDVLKEIRMEYVMPVIMLTAKEQEDDKVTGLELGADDYITKP
                     FSNKELIARVKANLRRVKLSKVEEEMKAKIVSVGNLTIDMNTYEVMKDGDIIDLTNRE
                     FDLLKYLFQNSDRVFNREHLLKEVWGYEFGDLRTVDVTVRRLREKIESDEDKYIITKR
                     GTGYYFKSK"
     gene            11048..12829
                     /locus_tag="PKK75_04275"
     CDS             11048..12829
                     /locus_tag="PKK75_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ82843.1"
                     /translation="MFKSIRWRFILIYFLLVFLAMSIVGIYIVNQLEEIQLDMNIKNM
                     EARIRSIIESYSSLNSGVWDENVEEIKKSISTVQVGYNENIYVILNDNNRTIIAGSVD
                     ESIGISAFNYNIIDNYILTKSMDGSSHHIAPAEQFEDTQNKRFYYHMSFPVQTDGEIR
                     GYIYLINNIDYIYDTVDESRMIMTQATIIALTLTIFLGLVLSTSITGPIKELTVKARQ
                     MSLGDFDQKVNVKSDDEIGQLGNMFNFLIDQLKRSMSSLQQEKSKMETTFTYMADGVL
                     TVDLSGNIIHANPVAKNILSLESTDQKFDDIIVNLKDNISMDHIKSRAYRGTDLAEKK
                     GETYNIDYAPFMNNKNEIGGVILVLKNITEQHRIDKLQKEFVANVSHELKTPITTIKS
                     YSETLLDGALEEKNTAVEFINVINSESDRMSRLVNDLLRLSKMDYEQTKWKKDSINLS
                     DIVNIIVKKLQIQIKDKNISMHLNIPEDINILFDRDGLEQVLLNITGNAVNYTPEGGN
                     VWINAKKDSNNTLVSIKDDGPGIPKEDQDRIFERFYRVEKARARGLGGTGLGLSIAKE
                     IAEANGCAIAVKSSLNKGTEMIITIPE"
     gene            12838..14259
                     /locus_tag="PKK75_04280"
     CDS             12838..14259
                     /locus_tag="PKK75_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82844.1"
                     /translation="MLKIRIKNTILLFMVVICIYLSSNVWLKLPEFFSPNAYEDESME
                     ETEADIWKVLRPVKNIIKYDDNYTVLYSDEIGLWQEALNVINDAFNDSNGTVTETEAF
                     PAEYLKFDFNTNIPVEIFTTHMKIENNDVDEIVKSIKNIIIDLKDNNSIYIYNGENNI
                     KVTSDKSSSKKLTDMVRSIDFERRTKYSMQEKAGDETIQVPVPLEQTALNPVFVQSEL
                     DVFDIDTINEIARDYFRNDYDYVRKSVEVSGNLVYVYRTEKILKINEDGLLDFYDASI
                     EPDNELDVYKSFAAAVNFIEEFLGFPENGYLSNVENILLEGNDGYRFTFSYNILERPI
                     LFSRVRENAALQIDVAGNNVISYKRFIRNIDDAQGYKMAQIQVLPAVEVIERNIDRST
                     AGEDNPANETSEQATEETAASIRPLKQEMIKEINNIYLGYFDLSRISKEQVLRVVWVI
                     ETNEKTFIFNAISGALIEEWQGE"
     gene            14261..15091
                     /locus_tag="PKK75_04285"
     CDS             14261..15091
                     /locus_tag="PKK75_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82845.1"
                     /translation="MDWNKTNTILITAFLILNIFLFVSSGNMIRADYNPNKDKEFAQE
                     VSELLDEKNIKINAVIPAETYTLPVLDTEYEIIHIDSQFLENFLHEKIEPLEDVYKYS
                     NKKGEILEVIDGKKLRLELRSGVPGKIDGDLNIKEKIENFIKDYNIDAGGYAENLKHI
                     SENRCNYIYTKRYNDYSVENSYMKFFIDKEGIYKFEMQKIISISEITEKIRTIHAIEA
                     LPRILTYPEIKNKEIAKIEMTYYSVEDENWQNIERINSDPTWKVIFSDGTQIHLPGIE
                     "
     gene            15115..16374
                     /locus_tag="PKK75_04290"
     CDS             15115..16374
                     /locus_tag="PKK75_04290"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNZ82846.1"
                     /translation="MSKFVIEGGTKLKGKVNIGGFKNAAVAIIPATILCSGKCIIENV
                     PKIKDVNVFLQILRELGAQADYIDDTSLYVDAANIKNCYLENGLSKKMRASYYLLGAG
                     LGRFKGASSLMPGGCDIGERPIDQHIKGFEALGAQVDIAPDGRITVQADKLTGAKIYL
                     DVVSVGATINIMLAAVYAEGKTIIENAAKEPHIVDTANFLNSMGADIKGAGTDVIRIN
                     GVRELTGCTYKVIPDQIEAGTYMVAAAATKGDIIIDDVIPKHLEPITAKLKEMGMGIE
                     EYGDSIHVYYDKELVPVNIQTLPYPGFPTDLQQPMTVLLSSLSGESTVVEGVSEDRFK
                     YVDELIKMGADIEFTKKQAKITGNRSLKGTVVQATDLRAGAALIIAGLIAEGLTEVRN
                     IEHIDRGYEHIENKFMNLGAKIKRVED"
BASE COUNT         5872 a         2418 c         3399 g         4767 t
ORIGIN      
        1 gaaaatatgt ttttctttca tttcttgcct cctcaattat attttcatta tatgaggggt
       61 ttttaaactt gttaaattaa attctatatt tacaaattta atggcacgct aattgcaaat
      121 ataattttaa aataaataat tttatatttt tgtctttaat gtctgttata tggattatta
      181 ttgttaatcg aataaagtgt tttttaatta ttttatattt gcagtcgtat taagcatttt
      241 atgtagtaaa agttaacata atcagttaac ataatgaaca aaacagtata atatctgaaa
      301 atcaattatc ttatggagac aaattatgga tgtgttttta tattttggga tgttattagc
      361 tttaacactg attatagctg cagggatata ttcagggaaa aaagtaaaag tttcgtctga
      421 tttttctgtt ggcagccgaa aagcaggata tatgattgtt gcaggaacta ttaccgggac
      481 tcttgtcgga ggtgcttcaa cagttggaac gtctgaatta gcatttacct acggattctc
      541 tgcatggtgg tttaccttgg gagcaggaat aggatgtttg ttgttaggtc ttgtatttgt
      601 tgaatctata tatcgaaacg gtaatgaaac agtttctcaa atattagctg atgaatacgg
      661 aaataaggta ggtctggttt catcagtctt tgtatcgatt ggtatgttta ttaatattgt
      721 agctcaaata ctttcagcaa cagcaattat cgtctctgtg tttaatatta caagctttac
      781 agcttcatgt attgccgcct tattgatgat tatttatgtt gtgtttggcg gtgtatggag
      841 tacaggcatt gttggaattg tgaaactgct gctattgtac atatctgtaa taataggagg
      901 cagcatggct ttaaaattag gtggtggaat ttcgcatttt attgaagtgc ttccatcaga
      961 gcaatatttt aacctgtttg ccagaggtaa gagcattgat ttgggggcag gattttcatt
     1021 gatagtgggt gtattgtcaa ctcaaacata catacaggct gttctaagcg gaaaagataa
     1081 atcagcttca caaaaaggag cattgctaag tgctgtgctt atacctccca taggcttggc
     1141 aggaatattt ataggaatgt atatgaagtt gaattaccct gatatagcac catcatccac
     1201 attacctcat tttgtcctga attatatgcc gccatttgtt tcaggaatta taatggctgc
     1261 tttgctggta acggttgtag gtaccggagc aggattggcg ttaggaataa gtactgttat
     1321 tactaaggat gtttataaaa actatataaa aagaaattca gatgaattaa cgcttttaaa
     1381 tatttcaaga ttattgattg ttgttgttct tgcagcagct gtgatattta ctgcaggaaa
     1441 tatgaaatcc tacatattgg aatggagttt tatgtcaatg ggattaagag gtgcagctgt
     1501 ttttgtaccc ttgtgcagcg ccttcttcct gaagggccgg ataaccagtc gatttgcttt
     1561 ggctggcgtc ttattaagtc ctgtttcggt tttaatcgga aaaactgtca tgcatacaga
     1621 aatagactca ttatttattg gtattgcagt taatgtcata atttttacga caggaattct
     1681 gtcgaataaa aaagcagaca taaactgaat ttttggttat actttgtaaa gtttatcaat
     1741 aaatatttgc attttattat atttcaccat ataattacat aacaatatat aagaggatag
     1801 aattatgcag aacagaatat tattggtttc atcaaatgat gagcttacaa aattagctga
     1861 gaaagtatcc aaaaatttaa atataaatta tgacttgtat gaaggcggaa ttacaaagga
     1921 tgggcatatt catgccaaga ataactccca caaatatgat gtaattatca gcagcggagg
     1981 cacagctgaa gctatccgaa aaattgtaag caagcctgtt gtttcggtgg aaataagtgc
     2041 tgcagatatt ctatatgcta tttataaagc gaaaaaattc aaaaataaaa ttggtatcgt
     2101 agtatataaa aatgatgaac ttgctcagtt ttacaagttg aaaaattttt taaatttaga
     2161 ctgtgaaata tttgaatatg aaactaaaag cgaaataata aataagatgg ctgatgccac
     2221 atcattagga aggcttacat tggtaggaat aggaggatgt attaaggaaa cagccatcga
     2281 tcacaatttg gattatgtca tcattgaaag cagtgaaaaa agcataaggg acgctcttat
     2341 aaatgctgca aatatatgcg aactgacaaa aaacgataag gaaagagcaa aaagatatca
     2401 agcaattctt gattattcag gagaaggaat aatttcttac gataaaaata acaaagtaac
     2461 atccataaat gcagctgcgg aaaagatatt ggatgtaaaa tataatgatg tattcaataa
     2521 gagcattgag gcaataaacg ataatttcaa agtgcttctt ggagatggaa acaaattaga
     2581 taataagctt acaaaaataa acggcaaaca ggttcttgtc aacagaatac ccataattat
     2641 tgacaatgac actcttagtg ttgtagtaaa tatccaggaa acctccaata ttcagaaaat
     2701 cgagagaaat ttgagagcag agctttaccg caagggactt gtggctaagc acacattttc
     2761 tgatataatc ggcaatagtg aaattatgaa agacactatt aataaagcta agagaattgg
     2821 gaaaacagaa agtactattt taattacttc tgaaacaggc tgtggcaaag agctgtttgc
     2881 acatagtatg cataatataa gcagcagaaa cggaggtcct tttgtagcag taaattgtgc
     2941 tgcacttcct gaaaatcttt tggaaagtga acttttcggc tatgaagaag gggcttttac
     3001 aggtgcaaaa aaaggcggta agatcggaat gttcgaactt gcccacaagg gaactatttt
     3061 tcttgatgag ataagtgaaa ttcctttgga tcttcaaggg agattattaa gagttttgca
     3121 ggaaagagaa atacaaagag tcggcggtga ttctgtagtt aatgtggata taagaataat
     3181 agttgcttcg aataaaaatt tatttgaaaa ggttattaat ggtagcttta gggaggactt
     3241 gttttttaga atcaatgtcc ttgatttgta tatacctcct ataagagaaa gaaagcaaga
     3301 catacctcta cttttggata attgttttaa aacaatagga agcagaataa gagttaataa
     3361 ttttagcagg gaagcattaa attggattat gaaatatgac tggaagggaa atgttaggga
     3421 aatagagaac tttgctcaaa gaatgtttgt attgcttgaa gactgcgcag atataaatga
     3481 aaatagctta gaaaatatta taaattataa attaataagt agtttaagca cgaaatactc
     3541 tcttgaaaac aaggataata tcatacaaat agaaataggt aatattaaag aaatggagag
     3601 taagataatt ttggaagcag ccaaaaagta tagcggtgac aaaactatgt tagctgataa
     3661 acttggcata agtagaacaa ccttgtggaa aaaactaaaa gaggcggatt gacttgttaa
     3721 aaaaataaac aaatataaaa ataattaaca agaataaata acacgaaagt ttttgactag
     3781 agaaatcaag taattgtaac aaaaattgct tgattttttt ttatgcagtt ggcacgtcaa
     3841 ttgcaataac ctttatagat aaatcttaat agataaatcg aaataaatat taatattaaa
     3901 aggaggcaat atgtcagagc aaggtgcaaa aattaaagaa aaaaagaaat tcaaggtacc
     3961 gcatacttag gtaattatat tttccattgt tttagtcatg gctgtgctga cctatataat
     4021 tcccgcaggc gaatttgtca gagttgaaga cgcagcaaca ggaaggactg taattgaccc
     4081 tacttcttat catgctgttg accagaatcc cacaactttc ttcgaactat ggaaggcagt
     4141 accaagaggt atggtggatg cggcgtcaat aatatttttc atgtttatta tttctggttc
     4201 attcggcgtt atttcatcaa caggagcaat tgaagccttc attagcaggg tggcattgat
     4261 gcttaaaggc aaagacaaaa tcatggtacc gatgattgtc ttcattttct caatattcgg
     4321 cggtacgata ggtatggctg aagaaacaat aatatttatt ccgttaggaa ttgttttagc
     4381 gagagcctta ggctatgatg caatagtagg aacatccatg attcttttag gctgtgcatg
     4441 cggttttaca tccggattca tgaattcatt tacagtaggt gttgcacaag gactggcaca
     4501 actgcctttg ttttcgggaa ttggattaag aattgttatt tggtttgtat ttgttatatt
     4561 cacaagtgta ttcattttaa gatatggagc caaaataaag gcagatccaa gtaagagcat
     4621 ggtatatgag ttagaggtag ctgagaagga tactatagtt gacttaagta atttgacaga
     4681 aatgaaaaga gagcattatt tcgtaatgat aacaatgata attgggttca ttgcaataat
     4741 attcggtgta tttaaagaag gatggtacat aactgaaatt tcagctgcat ttttggcaat
     4801 gggagttatc agtgggcttt tggggaaaat gggtcctagt aaaatagcac aacaatttat
     4861 tattggcgct aaagatattg tgtttggagc attagtagta ggtatagcaa gaggcatcct
     4921 gatggttatg aaggatggtg taataattga tacaatagtt aacacacttg caatgataat
     4981 acagacactt ccaagatcaa tatctgtact aggaatgtac atagtgcagg taattttaag
     5041 tataatcata ccgtccggaa gcggtcttgc tgcaacaact atgccaataa tggtaccatt
     5101 gtcagatgtt ttggaaataa acagacaagt tgccgtatta acttatcagt ttggtgatgg
     5161 tatatcaaat tctttcatac ctactgccgc atcatgtatg ggtgcattgt cagtagctaa
     5221 gatttcttat gaaaaatggt taaaatggtc agtgccatta ataataggct ggttgatttt
     5281 aggaggtata ttctgtatga taggaatggc aataaactac gggccattct aagaaaaaaa
     5341 tcacattatt aaactatttg attatcatat ttttattaag gaggaattat gatggaatac
     5401 actgagagta tgaatttaac tgaagctgtg aaactaactc aggaactggt aagaatacca
     5461 agccataaag acgtagaaga tagggaatca gcagtggcag aattcattta taattattgc
     5521 aaagaaaatg gacttgaagt tgagtatcag gtagttgaag gtaaacgtag aaatgtgatt
     5581 gcaaggctta gaggaaatgg aacaggcaaa aacctaatgt ttagcgggca tattgacaca
     5641 gtaccgccat atgatatgga atatgatgat ccttattgtg ctgatattgt tgacggatat
     5701 ttgcttggaa gaggctgcaa tgacatgaaa ggtgctgttg catgcatgat tacagcaatg
     5761 gttaatatta agaataaagg gaaagtttta aacggtgaca ttattttagc agcagttgta
     5821 ggcgaagaag aaaaaagtga cggaacagca tttgttgtaa aaagcggaat tactgctgac
     5881 ggagctatcg agggagaacc atcaaactac gggtattctt taggacatag aggacttgaa
     5941 tggattgaaa taaagttcat aggaaaaaga gctcacggat gcactccgga gttaggcata
     6001 aatgccatat ctaaggcatc aaaattcatt caaaagatag aaacagagct tatgccaaag
     6061 ttaaaagaaa gaaaaaacga atggatggga ccggatataa tgaattttgg atacattcat
     6121 gggggcgcag cacctagctt tgttgcagat aattgtgttc ttcaatttga cagaagatat
     6181 actcccggag agacagttga tgatgttatt aaggattatc aagatataat cgatgaatta
     6241 aaggctgaag attcacaatt taatgcagaa ataatcagaa tggaagaaaa cctgatggat
     6301 gattttgatc atgcaccatt aatagcagag cctgattcac aaattgcaaa aacagtttac
     6361 aaagctataa aagaatttat aggcaaagag cctgagatag tacaaagagg atggacagat
     6421 gccggacttc tttcaacata tggaaagatt cccactgttg ttacaggtcc gggtaatgtt
     6481 aaatggtctc atgcaaagga tgaaaaaatt cctgtcgctg atttagtaaa ctatgtagaa
     6541 atatatacaa atatagccga gcaattttgt atttaatgta aatgatgatt tattaataaa
     6601 aatgagattc ttaaaactat gcatatatgt ttatgtatag caaatagcta tatttggttt
     6661 tataagaatc tcttttatat tatttagtaa taaatttatt ataaaaagaa ctttcaaggg
     6721 cataatattt ttgaaagtat atctttcaat aatgaataag gagacataat atgcaaggca
     6781 aagtacaaaa aatggaccat cataatcccg ggattaaatg tgttgtgaac acatgttact
     6841 attattccga cggagatcac tgcaacgctc agaaaattga agtgcaaaag cgcgatgctt
     6901 ctacatccaa ggaaacagat tgtgcaacat ttacaaacaa ccatcaaagc atgtcataga
     6961 tgacgatgat ctaacccaag acttcgaccg atagggagaa agaaattgat ggtatgtcaa
     7021 atgcacaaaa ttcctaattt atgcgatgca ggagcaaata gaccagtgaa tttacctgca
     7081 agccctaatc gagactgcaa ccgtaaaatt gccctatatt tatagggcaa tttttctaat
     7141 catggacatt cctattgatc tcataggcta tcctttaact aaaggtgcgt ccccatacct
     7201 tattattgat attaatacga atatgtgtta atatgaatca aatgggggaa gtatgaaaaa
     7261 aattacgaaa atagaatacc agaagaagaa taaagaaaga ttaaatattt acatagatga
     7321 tgaatacgga ttttctgttg acataagcat attgattgaa tattcactta aaaaagacat
     7381 ggtcttggat gattccctca ttgatgccat attgaggtcc gaagagagaa caagtgttta
     7441 caattacggg atttccgtcc tctcaagata tgccagaagc gaatatgaat taaggcttaa
     7501 gatgaaaaac aagggatttg agcctgaact aatagacaag gctgtgggta cccttaaaga
     7561 acaaaagtac ctggacgatg atagatattg tgagatgttt ataaatgata aaatcaacat
     7621 ttcaaaagac ggtgtgcgta aaataaagga agctctatat tacaagggaa ttgacaaaaa
     7681 aataatagag gaaaaaatta agaatatttc tcaagacagc gaagaagaaa gagctttaat
     7741 actggggagc aaaaaacttc caaacataaa ggaaaccgat acccgtaaaa aaatagcaaa
     7801 gttgtcgaac tatttaatag gaaaaggctt tgaatatgaa acggtaaaca agacagtaag
     7861 aaaactttta agtatagagg ctgatgattt ctattgaggg gggatatata tgaaagagtt
     7921 tgcagaatac atatcagatg cattcgttca ttgcaggatg atctgctatg aggggaaatt
     7981 tgatattgta gcaatttatg caaataagca gctggagcaa attgccagca gaacgctgga
     8041 ccagttaatc ggcagaaaaa tgacagatgt gtttcccaat ctttcagatt caatttttga
     8101 ctggcctaaa atattgtgtg aagctgcaat gaccagtgaa cacacagtca ttgaacaata
     8161 tgtaaatgct tttgaaaagt ttgtcaggtt taatgtattt ggatttaaag atgatacttt
     8221 ttatgtgaca attcaagatt taactaataa aagagaaatc aggcggataa ttcttgaaaa
     8281 ggaccgacaa attgaacatt tggaaaatga aataaaatcg agagcaagca ttgagcctct
     8341 tacaaacctg tataactttc agtttattac cgactgcata aaacaaagca tacaatgtta
     8401 caaggaagaa ggaactgtat tttgcatatt tttaatagat attgacaatt ttaaagcctt
     8461 caacctgaaa tacggcatgg aaaccggaga catggttttg caggatatag cacatatact
     8521 cagtttgtcc gccagaaaaa tagatgtggt tggcaggtat ggaaatgata aatttataat
     8581 gatattaaat aatgtagagc tggatattgc aaagataatt atcgaaaagt tcaaaatgga
     8641 aatcggcaga tatgacttga atataaacaa aagcaatatt tatgtaagcg gagctgttgt
     8701 tgaatacagg ggggaaagcc tggaagaatt cattgaaaaa gctgagaaac tgctgtcaaa
     8761 ggctcaatcc atgggaaagg gaaaaatatt gtcatagcat aagggagcgc aagctccctt
     8821 tgttatgaaa aaatacgcct catatctaga acacctgcac cttgctcata ggaggatgct
     8881 tttattttag agcatgaatt aaggagtatt ctctttatgt cgaagtggct gtaatcaggg
     8941 ttttcattca acagcagagc acaaacccct gatatcatgg gtgctgacat cgatgttccg
     9001 ctcaatgtgg tgtagccgtt taagttttta ctggataaag aaataatatc aactcccgga
     9061 gctattaaat ccggtttgcg tactctgtca agtgtgggtc ctctgcttga aaactcggca
     9121 attgtctcat ttttaggata agacgtccct ctgtcgttta cagatcctac ggaaataaca
     9181 tacctgcttg ttgcaggaga taatattgta ttgtgcatag gcccgctgtt gcctacagcg
     9241 gcaactacta tgaggtcttc ttcgattgct ctgtttgctg cctttaccaa cgggtccctc
     9301 ctttccttat actgggcgat tgcccccagg gaaaagttta ttattttagt tttataaacc
     9361 tccttgcttt caataatcca ttggactgtt gacagaatat ccgatgtatt tccatttccg
     9421 ttttcgtcaa gtaccttgat tgacagtata tttgcttccg gcgctattcc tttgtatctg
     9481 cccttggatg aatacccgct gcctgcaagt atccctgcgc agtgggtgcc gtggccgttg
     9541 tcatcgtaat aattagacct gttgttgaca aaatccttaa agcctactat tctttttgca
     9601 ggataaatca aatctgcatg tggtgctata cctgtatcaa ttattgcaac agttacacct
     9661 tttcccgtgt acttggtatt cagagaataa tcggctccga ttgtttttct tgcctcttcc
     9721 attacaacaa atacttttga atcataggat atgaattcga catccgggtc ggatgttaat
     9781 cgaaggattg aatcaatatt catttcacat gcacagccgt tgattatggg aagactgtgt
     9841 ttcaatttgc ctgataagga actgatctct ccctccttta tcttcttgct ggatttataa
     9901 cttataataa cgggtatact aatattgctg cttgatgaaa gctgtccctt tatgacggga
     9961 catactttat ttatatctgc actgtttctc attaaattca cctacctaat ttaatttatg
    10021 cttttaaata aagatggtgt tcatttttga aaaacatgtt ataataaata cgataaatga
    10081 agcgaagact taaggtcttt gcccctgtgc atttattgta tcggtatgga gcaagccgat
    10141 tttacagctt gaattgataa tataatattc atgataatga gacagaaatt aaagcgagag
    10201 gtgaaataat gcctaagata ctggtagtag atgacgaaag acctatagct gaaataatta
    10261 aatataatct tcaaaaggaa ggctatgaag tgcagacagc ctatgacggt gatgaggcta
    10321 tcaaggcagt ccaccaaatg gaaccggaat taataatact tgacataatg cttcctaaga
    10381 aaaatggatt tgatgtttta aaggaaatta ggatggaata cgtaatgcct gtaattatgc
    10441 ttactgcaaa agaacaggaa gatgacaagg tcacaggact tgaactggga gctgacgact
    10501 atattacaaa gcccttcagc aacaaggaat taattgccag agtaaaggcc aacttaagaa
    10561 gagtgaagct ttcaaaggtt gaagaggaaa tgaaagcaaa aattgtctct gtagggaatc
    10621 ttaccattga catgaacaca tatgaagtta tgaaggatgg agatataata gatcttacaa
    10681 acagggaatt cgatctttta aagtatttgt ttcaaaattc ggacagggta tttaacagag
    10741 aacaccttct caaggaagtc tggggatatg aattcggcga tttaagaaca gttgatgtga
    10801 cggtaagaag acttagagaa aaaattgaat cggatgagga taaatatata attacaaaac
    10861 ggggaacagg atactatttt aagagtaaat aggagatgcc ctaaccctat tttttagagt
    10921 gttttttcta aaaaatagcg gtaggtcatt cgcaatgaat tccaaaaata tacgaccaaa
    10981 gggagtagat atttttgtga atgagactgc gtgctttgaa atattggcat tttcaccagg
    11041 agacattatg tttaaaagta taagatggag atttatttta atttattttt tactggtttt
    11101 ccttgccatg tccatagtgg gcatttatat agtaaatcag cttgaagaaa ttcagcttga
    11161 catgaatata aaaaacatgg aagcccgaat tcgttcaatt attgaatcct acagctcatt
    11221 aaattcagga gtatgggacg aaaacgttga agaaatcaaa aaaagcatca gcaccgtgca
    11281 ggtaggctac aatgagaata tctatgtaat cttaaatgat aacaacagga caataattgc
    11341 cggcagcgtg gatgaatcaa tcggaataag cgccttcaac tacaatataa tagacaatta
    11401 catactgaca aaatcaatgg acgggtcttc tcaccacatt gcaccggcag aacaatttga
    11461 agatactcag aataaaagat tctattatca catgtctttc cctgtgcaga cagacggtga
    11521 aattcgaggc tacatttatc taatcaataa tattgattat atttatgata cagtggatga
    11581 atcaaggatg ataatgactc aggcaacaat aatagccctg accttaacta tatttttagg
    11641 attggtactg tcaacaagca taacgggacc cataaaggaa cttactgtaa aagccaggca
    11701 gatgtcactg ggtgattttg atcagaaggt aaacgtaaaa tcagacgatg aaataggtca
    11761 actgggcaac atgttcaatt tccttatcga ccagctaaaa aggtcaatgt caagtcttca
    11821 gcaggaaaag agcaagatgg aaaccacctt cacctacatg gccgacggag ttctaaccgt
    11881 tgatttatca ggcaatatta tacatgccaa cccggtggca aaaaatattt tatcccttga
    11941 aagtaccgac cagaaattcg atgatataat agtaaacctc aaagacaata tatccatgga
    12001 ccatataaaa tccagggctt atcgcggcac ggacctggca gaaaagaaag gggaaaccta
    12061 caatatagat tacgctcctt tcatgaataa caaaaatgaa atagggggcg tgattcttgt
    12121 acttaaaaat attaccgagc agcatagaat tgataagctt caaaaagaat ttgtcgcaaa
    12181 tgtatcccat gaattaaaaa cacccataac aacaatcaaa agctattcag aaacactttt
    12241 ggacggggca ctggaagaaa aaaacacagc ggtagaattt ataaatgtta taaattcgga
    12301 gagcgacaga atgtcaaggc ttgtaaatga tttgctccgc ctttcaaaaa tggattatga
    12361 gcagactaaa tggaaaaagg acagtattaa tttatcggat atagttaata ttatcgtaaa
    12421 aaaactccaa attcaaataa aagacaaaaa tataagcatg catttaaata ttcctgagga
    12481 tataaatatt ttgtttgaca gagacggatt agagcaagtt ttattaaata ttacaggaaa
    12541 tgctgttaat tatacacctg aaggtggaaa tgtctggatt aatgcaaaaa aagacagcaa
    12601 taatactctt gtaagcatta aagatgacgg gccgggcata ccaaaagaag accaggacag
    12661 gatttttgaa agattctatc gtgttgaaaa agctagagcc aggggcttgg gaggaacagg
    12721 tctgggactg tcaattgcca aagagattgc agaagccaac ggatgtgcaa tagcagtcaa
    12781 aagcagttta aacaaaggga ctgaaatgat tattacaatt cctgagtaga ggatattatg
    12841 ttaaaaatta gaataaaaaa tacaatattg ttgttcatgg tggtcatatg tatatattta
    12901 agcagcaatg tatggcttaa gcttcctgaa ttcttcagcc ccaatgctta tgaagatgag
    12961 agcatggaag aaactgaagc ggacatatgg aaggtactca gaccggttaa aaatataatc
    13021 aaatatgatg ataactacac tgttttgtat tcagatgaaa taggattgtg gcaggaagca
    13081 ttaaatgtaa taaatgatgc ctttaatgat tcgaatggca ctgtaaccga aacagaggca
    13141 tttcctgcag aatatctgaa atttgacttc aacaccaata ttcctgttga aatatttacc
    13201 acccacatga aaattgaaaa taatgatgtt gatgaaatcg taaaaagtat aaaaaatatt
    13261 ataattgact taaaagacaa taattcaata tatatttaca acggtgaaaa caacataaaa
    13321 gttacaagcg ataaaagctc ttcgaaaaaa ttgacagata tggtgaggtc aattgatttt
    13381 gaaagaagga caaagtattc catgcaggaa aaagcagggg atgaaacaat acaggtacct
    13441 gttcctttgg agcagactgc actcaacccg gtttttgtcc agtcggagct tgatgtattc
    13501 gatattgata caattaatga aatcgccagg gattatttca gaaatgatta tgactatgta
    13561 agaaagtctg ttgaagttag cggaaacctg gtttatgttt acagaacgga aaagatactg
    13621 aaaatcaacg aggatggtct cctggatttt tacgatgcat caattgagcc ggataatgag
    13681 ctggatgttt ataaaagctt tgctgcggct gtgaatttta ttgaagaatt ccttggcttt
    13741 ccagaaaacg gatatttgag caatgtagaa aatattcttt tggaaggtaa tgatggttac
    13801 aggtttacat tttcctacaa tatattggaa agacctatat tattcagcag agtaagggag
    13861 aatgcagctc ttcaaatcga tgtagcagga aacaatgtta tatcatacaa aagatttatc
    13921 agaaacattg atgatgcaca agggtataaa atggcccaaa tacaggttct tcccgctgtt
    13981 gaagtaatag aaagaaatat tgacaggtca acagccggtg aagataatcc tgcaaatgaa
    14041 acatctgagc aggccactga ggaaacagct gctagtatca gaccgttaaa gcaggaaatg
    14101 ataaaagaaa tcaacaatat ctatcttggg tattttgatt tatcgcgaat ttcaaaagaa
    14161 caggttttaa gggtggtatg ggttatagaa accaacgaaa agacatttat attcaatgcc
    14221 atatcaggtg ctttgattga ggagtggcag ggagaataac atggattgga ataagacaaa
    14281 tacaatttta ataacagctt tcttaatctt aaatattttt ctttttgtat cttcaggcaa
    14341 tatgataagg gcagactata atcctaataa ggacaaggaa tttgcccaag aggtgtcaga
    14401 acttttagat gagaaaaaca taaaaataaa tgctgtaata cctgcggaaa cttatacact
    14461 gcctgttttg gatacagagt atgaaataat tcacatagat tcacagtttt tggaaaattt
    14521 tttacatgaa aaaatcgagc ccctggagga tgtgtacaaa tacagcaata aaaagggaga
    14581 aatcctggaa gttattgacg gcaagaaact gagactggaa ctgcggagcg gggttcccgg
    14641 caaaatagac ggagatttga atatcaagga aaaaattgaa aactttataa aagactataa
    14701 tattgatgca gggggatatg ctgaaaattt aaagcatatt tcagaaaaca gatgcaatta
    14761 tatatataca aaaagataca atgattattc tgtagaaaat tcctatatga agttttttat
    14821 tgataaggaa ggaatttata aatttgaaat gcaaaaaata atctcgattt ccgaaataac
    14881 cgagaaaatc cgcacaatac atgcaataga agcattaccg agaattttaa cctatccgga
    14941 aataaaaaat aaagaaattg caaaaataga aatgacatat tatagtgtag aagatgaaaa
    15001 ttggcaaaat attgaaagaa taaattccga ccccacgtgg aaagttattt ttagcgatgg
    15061 aacacaaata catctgcccg gaattgaata aaaaataaac tgtgaggaag tactatgtca
    15121 aaatttgtta tagaaggcgg cacaaagctt aaagggaaag taaatatcgg aggattcaag
    15181 aatgcagctg ttgcaataat accggctaca atactctgct ccggaaagtg catcatagaa
    15241 aatgtaccaa aaataaagga cgtcaatgtt tttctgcaaa tattgcggga attaggtgcc
    15301 caggcagact atatagatga tacatcattg tatgtagatg cagccaatat aaagaattgc
    15361 tatttggaaa acggattatc caaaaaaatg agagcatcat attatctgct tggtgcagga
    15421 ttgggaagat ttaagggagc ttcctcactg atgcccgggg gctgcgacat aggggagagg
    15481 cccattgacc agcatatcaa ggggtttgag gcattaggag cacaggttga tattgctccc
    15541 gatgggagga taaccgtaca agctgataaa ctaacaggag caaaaattta ccttgatgtg
    15601 gtaagtgtgg gagctaccat aaatattatg ctggcagcag tttatgcaga gggtaaaaca
    15661 ataatcgaaa atgcagccaa ggagcctcac attgttgata cggcaaactt cttaaactca
    15721 atgggagcag atatcaaggg agcgggaaca gatgttatac gtataaacgg cgtgagggaa
    15781 ttgacaggct gtacatacaa ggtaataccg gaccagattg aagcaggtac atacatggtg
    15841 gctgccgctg ccacaaaagg agatataata atagatgatg taatacccaa gcacctggag
    15901 cctataactg caaagcttaa agaaatggga atgggcatag aagaatacgg tgattcaata
    15961 catgtttatt acgataagga attagttccc gtaaatattc aaactcttcc ctatcccggg
    16021 tttcctacgg acctgcagca gcccatgaca gtgctcttat cctcattatc cggcgaaagc
    16081 actgtagttg aaggggtatc cgaggacagg tttaaatatg tggatgagct gattaaaatg
    16141 ggagcagaca tagaatttac taagaagcag gctaaaataa cagggaacag aagcttaaag
    16201 ggaacagtgg tacaagctac agacttgaga gcaggagccg ccttgattat tgcaggcctc
    16261 atagccgaag gattgacgga agtaagaaat attgagcata tagacagagg ctatgaacat
    16321 attgaaaaca aattcatgaa cttaggggca aaaattaaaa gagtcgaaga ttaatcacag
    16381 ggggtttact tttgaataag gttgtattga ttcctgattc ctttaaggga acattaacat
    16441 ctattgaaat ctgcac
//
LOCUS       DAOPUT010000031        16028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3718_length_16028_cov_18.513366, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000031 DAOPUT010000000
VERSION     DAOPUT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16028
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3718_length_16028_cov_18.513366"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKK75_04295"
     CDS             complement(<1..678)
                     /locus_tag="PKK75_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ82847.1"
                     /translation="MAKAKFERNKPHVNIGTIGHVDHGKTTLTAAITLILNKRFGTGE
                     FISYDNIDKAPEERERGITISTSHVEYETQKRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVSAADGPMPQTREHILLSRQVGVPKLVVFLNKEDQVDDPELIELVEMEVRELLS
                     EYEFDGDNTPIIVGSALKALEEPDGPWADKVIELMDAVDEWIPTPTRDIDKPFLMPVE
                     DVFSITGR"
     gene            complement(714..2774)
                     /gene="fusA"
                     /locus_tag="PKK75_04300"
     CDS             complement(714..2774)
                     /gene="fusA"
                     /locus_tag="PKK75_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ82848.1"
                     /translation="MREYSLENTRNIGIMAHIDAGKTTCTERILFYTGKIHKVGETHE
                     GAAQMDWMEQEQERGITITSAATTCIWKDERVNIIDTPGHVDFTVEVERSLRVLDGAV
                     AIFDAKMGVEPQSETVWRQADKYHVPRICFVNKMDKMGADFFYSVGTMLERLKANAVP
                     IQLPIGAEDKFNTIIDLVSMNATRYNNDEGTDLELIPIPGEYLDKAREYRDKLIEALS
                     DFNEEIMEKYLNGEEITVAELKKAIRTSTINNQIVPVLCGTAYKHKGVRLMLDAIVDY
                     LPNPLEVPQLKGFDPDTEEIIVRDASDDAPLSALAFKIATDPFVGKLTFFRVYSGVLE
                     SGSYVLNSTKNKKERIGRILQMHANKREEIKNSYTGDIDAAVGLKITTTGDTLCDPDH
                     PIVLESMVFPEPVIEVAIEPKTKAGQEKMGLGLQKLAEEDPTFKTYTNPETGQTIIAG
                     MGELHLEIIVDRLLREFKVEANVGKPQVSYKETVSGNADVDVKYARQSGGKGQYGHVK
                     IILEPQEMGQGITFVNAITGGVIPREYIPAVEAGVRGAAENGILAGYPVIDFKVTLYD
                     GTYHEVDSSEMAFKIAGSMAFKQAMEKASPVLLEPYMKVEITTPEEYLGDVMGDINSR
                     RGRIEGFSDRSGIKVVDAYAPLSEMFGYATTLRSLSQGRAAYSMEFHHYEKVPANVAE
                     KIIK"
     gene            complement(2812..3282)
                     /gene="rpsG"
                     /locus_tag="PKK75_04305"
     CDS             complement(2812..3282)
                     /gene="rpsG"
                     /locus_tag="PKK75_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNZ82849.1"
                     /translation="MPRKGHIPKREVMEDPIYNDKTVTKLINQLMYDGKKGVAQSIVY
                     GAFDIIKEKTGEEPLEIFNKAMNNIMPVLEVKARRVGGATYQVPIEVRPERRQTLGLR
                     WLVTYSRKRGEKTMRERLAKELMDAANNVGASVKKREDTHKMADANKAFAHFRW"
     gene            complement(3391..3807)
                     /gene="rpsL"
                     /locus_tag="PKK75_04310"
     CDS             complement(3391..3807)
                     /gene="rpsL"
                     /locus_tag="PKK75_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNZ82850.1"
                     /translation="MPSINQLVRKGREQVTYKSDSPALNVSYNTLNKRMNESNSPQKR
                     GVCTSVKTVTPKKPNSALRKIARVKLTTGMEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIIRGTLDTAGVAKRKQARSKYGVKREKKK"
     gene            complement(3835..4068)
                     /locus_tag="PKK75_04315"
     CDS             complement(3835..4068)
                     /locus_tag="PKK75_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNZ82851.1"
                     /translation="MVTDKKTVVGRKQTLRALSRDEAKIVYLSKDADLHVTKTITDVC
                     REKNIEIIYYDSMKELGKSVGIGVNAAAAAVLK"
     gene            complement(4202..7708)
                     /gene="rpoC"
                     /locus_tag="PKK75_04320"
     CDS             complement(4202..7708)
                     /gene="rpoC"
                     /locus_tag="PKK75_04320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNZ82852.1"
                     /translation="MVDYNNFESIRIGLASPDKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTKDWECHCGKYKRVRYKGVVCDRCGVEVTKAKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLILDMSPRALEKVLYFAQYVVTEAGDTSLSEKQLLTEIEYREYKE
                     HYKNAFKAEMGAEAIKKLLEKIDLESEAAELKSQLRDSKGQKKVRIIRRLEVIEAFRV
                     SGNRPEWMVLDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFNQCGLPKKMALELFKPFVMKKLVETGAAHNIKSAKKKVEK
                     VDTAVWDVLDEIIKDHTVMLNRAPTLHRLGIQSFEPVLVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQSESKFLMMSVNNILAPKDGRPITTPTQDMVLGSYFMTLEIED
                     SKGQGMIFTDYDELKMAYFNRNVDLHARVKVRRTVGDETRLVDSTVGRFIFNEDIPQD
                     LGLVDREKDKFSLEIDKTVTKKYLEEIIYRCFRKHGNSRTAEVLDGIKQKGFRYSTVG
                     AITISVSDITVPEEKKTLIEQAEKQVIEYEKKYRRGLMTDDERYDNVIKVWNQTTEDV
                     TNALMNNLSPLNPIYIMSVSGARGGRNQIKQLAGMRGLMASAIGKTVEVPVKSNFREG
                     LSVLEFFLSATGARKGLTDTALRTADSGYLTRRLVDVSQDVIVREADCGTTDGIEVED
                     FIDGKEEIERLSERCADRFAADDVVDPETGEIYVRAGDFISEDIALKIQNADIKKVKV
                     RSVLTCKTVHGVCAKCYGTNLATGKPVNVGAAVGIIAAQSIGEPGTQLTMRTFHTGGI
                     AAAADITQGLPRIEELFEARKPKGQAIISEIPGKITLTEKAHKREVTVTNYELMESKT
                     YLIPYGSKILVVEGDYIEAGDKITDGSINPHDILNIKGMKALESYILMEVQRVYRMQG
                     VDISDKHVEVIIRQMVNKVRIEDSGDTSLLPGSLVSNYEFNQQNETAVNEGKEPATGE
                     VALLGITKSSLATDSFLASASFQETTRVLTDAATKGKVDSLIGLKENVIIGKLIPAGT
                     GMKKYRNIGITTGDVEKAADVG"
     gene            complement(7748..11521)
                     /gene="rpoB"
                     /locus_tag="PKK75_04325"
     CDS             complement(7748..11521)
                     /gene="rpoB"
                     /locus_tag="PKK75_04325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNZ82853.1"
                     /translation="MPHPVKIGNRVRMSYSKINEVLELPDLIEVQKKSYNWFLNEGLK
                     EVFDDISPIEDYTGNLILEFVDHSLLNEPKFNQVECKERDATYSVPLKLKVRLINKET
                     GEIKEQEAFMGEFPLMTEKGTFIINGAERVVVSQLVRSPGVYYNMTRDKVGKELYDAT
                     VIPNRGAWLEFETDSNDIVYVRVDRTRKLPATVLVRAMDVSSNSEIINLLGETEQLLK
                     TLEKDNTTNSEEALIEIYKRLRPGEPPTYDSAKSLIKSLFFDFKRYDLAKVGRYKLNK
                     KLSLENRIIGKRAADDIIDKETGEVLVAKETTFDSDSIFKLEAAGIFSVNIVNTDDKV
                     TKILSNKFVDPKVFKLDELNIDLEKLGVKEKVYYPVMKKILEVNKTKEEIEKAIIESL
                     KQITAADNNIFEKDGEEVYSLSPKHIVKDDMYATVNYHFGLFKGIGKTDDIDHLGNRR
                     LRSVGELLQNQVRVGLSRMERVVRERMTIQDVDVVTPQVLINIRPVSAAIKEFFGSSQ
                     LSQFMDQTNPLAELTHKRRMSALGPGGLSRDRASFEVRDVHHSHYGRMCPIETPEGPN
                     IGLITSLATYARINDYGFLESPYRRVDKKTGKVTDIIDYLTADVEDTNIIAQSNEPLN
                     PDGTFEHSRVVVRGIDGINEIASKDMVDYMEVSPKQVISIGTGMIPFLENDDANRALM
                     GANMQRQAVPLLITESPIIGTGLEHKAAMDSGAVIIAEHDGTAVSVTASQILIKRSDN
                     GMIDKYTLLKFKRSNSGTCVNQRPIISKGDEIKRGQIIADGPSTHDGDMAIGKNLLIG
                     FMTWEGYNYEDAMLLNEKLVMEDILTSVHIEEYESEARDTKLGPEEITRDIPNVGDDS
                     LKDLDERGIIRIGAEVKSGDILVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLK
                     VPHGETGIIVDVKVFTRENGDELSPGVSKLVRVYIATKRKINVGDKMCGRHGNKGVIS
                     MIMPEEDMPYLPDGTPLQVVLNPLGVPSRMNIGQVLEVHLGLAAKKLGWEVATPVFDG
                     AVEDDIWDALELAGYPRSGKIDLRDGRSGEYFEGPVTVGYMYMLKLHHLVDDKIHARS
                     TGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQEILTVKSDDVNGRVKTY
                     EAIVKGENIPEPGIPESFKVLVKELQSLALDIKIINSDDEVEIIETMDNDDDLPVDTE
                     ALNDMTFVEDDFEGMQVEVEDIEEDIDDIEDIEIDSEELDDLDLEEDYDDEDF"
     gene            complement(11957..12337)
                     /gene="rplL"
                     /locus_tag="PKK75_04330"
     CDS             complement(11957..12337)
                     /gene="rplL"
                     /locus_tag="PKK75_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNZ82854.1"
                     /translation="MASEKITNFVEEIKKLSVLELNELVKAIEEEFGVSAAAPVAVAG
                     AAAGAAAPVEEEKTEFDVVLTAAGAGKIKVIKVVRELTGLGLKEAKDAVDGAPTTIKT
                     AVPKEEAEKAKAALEAEGASVEIK"
     gene            complement(12528..13031)
                     /gene="rplJ"
                     /locus_tag="PKK75_04335"
     CDS             complement(12528..13031)
                     /gene="rplJ"
                     /locus_tag="PKK75_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNZ82855.1"
                     /translation="MNQSVLEQKKQVVSEITEKIRNAKSVVLVEYKGLTVEKATLLRN
                     KCREAGVEYKVYKNTLMRFAFEELGYNGMSVNLEGPNAIALSYGDEVSAAKVTNDFAK
                     DSNDTLILKAGIAEGKILNADEVKELASVPSRDILLGQLAGVLQGNIRNLAYMLDQIS
                     KKGEEAA"
     misc_feature    complement(13213..13328)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(13337..14062)
                     /gene="rplA"
                     /locus_tag="PKK75_04340"
     CDS             complement(13337..14062)
                     /gene="rplA"
                     /locus_tag="PKK75_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNZ82856.1"
                     /translation="MPKRGKKYQDSIKSIEKLKLYDMDEAVEAMLSTAKANFDETVEV
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTVRVLVFAKGDKAKEAQQAGADFVGEGELAE
                     KIQKENWFDFDVVIATPDMMGVVGKLGRVLGPKGLMPNPKSGTVTFDVEKALQEIKAG
                     KVEYRLDKTNIIHVPAGKKSFGTEKIVDNLKVIIDAVLKAKPAAAKGKYLKSVTIAST
                     MGPGIKLNTTKLTDSFVSKTKAE"
     gene            complement(14119..14544)
                     /gene="rplK"
                     /locus_tag="PKK75_04345"
     CDS             complement(14119..14544)
                     /gene="rplK"
                     /locus_tag="PKK75_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNZ82857.1"
                     /translation="MAKKVTAVVKLQIPAGKATPAPPVGTALGPHGVNIMGFCKEFNA
                     KTADQAGMIIPVVLTVYQDRSFTFITKTPPVAVLIKKAVGIESGSGEPNKKKVATLSK
                     AKCEEIAKLKMPDLNANTVEAAMSLVAGTARSMGVKVEE"
     gene            complement(14590..15117)
                     /gene="nusG"
                     /locus_tag="PKK75_04350"
     CDS             complement(14590..15117)
                     /gene="nusG"
                     /locus_tag="PKK75_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNZ82858.1"
                     /translation="MKQESQGSRWYVVHTYSGHENKVKANIEKLVENREMQDTIFEVR
                     VPVEEVAEVKNGKKKIKERKMFPSYVIIKMIMTDESWYLVRNTRGVTGFVGPGSKPVP
                     LTDEEVKALGVVEKLPAIELEAGDTIKVKEGPFEDFIGTVDSISQEKRKIKAFVSMFG
                     RETLIELEYDQIEKV"
     gene            complement(15130..15342)
                     /gene="secE"
                     /locus_tag="PKK75_04355"
     CDS             complement(15130..15342)
                     /gene="secE"
                     /locus_tag="PKK75_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNZ82859.1"
                     /translation="MSNRDNEKPKFLTRVKNYFKGVRSEMKKVNWPTRKELQNYTIVV
                     LATTFALTVVIWGLDLIFRGLLTVIV"
     gene            complement(15355..15504)
                     /gene="rpmG"
                     /locus_tag="PKK75_04360"
     CDS             complement(15355..15504)
                     /gene="rpmG"
                     /locus_tag="PKK75_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNZ82860.1"
                     /translation="MRVAVKLACTECKQRNYDTEKNKKENPERIEMNKYCKFCKKHTL
                     HRETK"
BASE COUNT         3976 a         3784 c         2643 g         5625 t
ORIGIN      
        1 tcttcctgtg attgagaaca cgtcttctac aggcatcagg aaaggtttgt caatatctct
       61 tgtaggtgtg ggtatccact catctactgc atccatcagt tcaataacct tgtcagccca
      121 tgggccgtcc ggctcttcga gggcctttaa tgctgaaccg acaattatcg gtgtattgtc
      181 gccgtcaaat tcatattctg ataaaagttc tctgacttcc atctcaacca attcaattaa
      241 ttcagggtca tctacctggt cttctttatt caggaaaaca accagctttg gaacaccaac
      301 ctgccttgaa agaaggatgt gctctcttgt ctgaggcata gggccgtcag cagctgatac
      361 aaccaggatt gctccgtcca tctgggcagc tcccgtaatc atgttcttaa cgtagtcggc
      421 gtgtcccggg cagtcaacgt gtgcatagtg tcgtttttga gtttcatatt caacgtgaga
      481 agtagaaata gttattcctc tttctctttc ttcaggagcc ttatctatat tgtcatagct
      541 tataaattct cctgtaccaa atcttttgtt caaaataagt gtgattgcag ctgttaatgt
      601 agttttgccg tggtcaacgt gaccaattgt tcctatatta acatggggtt tatttctttc
      661 aaattttgct tttgccattt tttcctccct taggtgtttt tatatatgtt tttttatttt
      721 ataatttttt cagctacgtt ggcaggtacc ttttcgtagt gatggaattc cattgaataa
      781 gcagctcttc cctgggacaa tgaacggagt gtagtagcat atccgaacat ttccgataag
      841 ggtgcatatg catccacaac ctttatcccg ctcctgtcag agaaaccctc tatgcgccct
      901 cttcttgagt taatgtctcc cataacatct cccaggtatt cttcaggagt tgttatctcg
      961 accttcatat agggctctaa aagaaccggt gatgcctttt ccattgcctg cttaaaggcc
     1021 attgaaccgg caatcttgaa tgccatttcg cttgagtcca cctcatggta tgttccatca
     1081 tacagggtaa ccttaaaatc tattacaggg tagccggcaa gaatgccatt ttctgctgcg
     1141 ccccttactc cggcttcaac agcaggtata tattctctcg gaatgactcc gcctgtgata
     1201 gcattgacga aagtgatgcc ctgacccatc tcttgaggtt ccaggataat tttgacatga
     1261 ccgtattgtc ccttgcctcc tgattgtctt gcatacttaa cgtcaacatc tgcattgcct
     1321 gaaactgttt ctttgtaaga aacctgcggc ttgcctacat ttgcttccac cttgaattct
     1381 ctcaagagtc tgtcaactat tatttccaaa tgaagctcgc ccattcctgc aattatagtc
     1441 tgccctgttt ccggatttgt atatgttttg aatgtggggt cttcttcagc cagcttttgg
     1501 agtcccaatc ccatcttttc ctgccctgcc ttggtcttcg gctcaattgc cacctctata
     1561 acaggttcag ggaaaaccat ggattccaaa acaatgggat ggtcaggatc acacaacgtg
     1621 tctcctgtgg ttgtaatctt taagcccaca gcagcatcaa tgtctcccgt ataagagttt
     1681 ttaatctctt ccctcttgtt tgcatgcatc tgcaggattc tgcctattct ttccttttta
     1741 ttcttggttg aattcaagac ataagaacct gattccaaca ctcccgaata aactctgaaa
     1801 aatgtaagct tgcctacaaa ggggtcagtg gcaattttaa atgccaaggc tgaaagggga
     1861 gcatcatctg acgcatctct tactattatt tcctcagtat ccgggtcaaa tcctttcaac
     1921 tggggaactt ccaacggatt tggcagataa tcaacaattg catccagcat tagtcttacg
     1981 cctttgtgtt tgtaagctgt accgcataaa accggcacaa tttgattgtt tattgtggat
     2041 gttcttatag cttttttaag ctctgcaact gtaatttctt ctccgtttaa atatttctcc
     2101 attatttctt cattgaaatc cgataaagct tctataagtt tatctctgta ctcccgggct
     2161 ttatccaaat actccccggg tatagggata agttctaaat ctgttccttc atcattatta
     2221 tatcttgttg cattcataga aaccaaatca atgatagtgt taaacttatc ctcagctcct
     2281 atgggcagct ggataggaac agcatttgct tttaatcttt ccagcatggt gcctactgaa
     2341 taaaagaaat cagctcccat tttatccatt ttgttaacaa agcatattct cggcacatgg
     2401 tatttatccg cctgtctcca gacagtttcc gactggggct caacccccat ttttgcgtca
     2461 aatattgcta cagcaccatc caacactcta agtgaccttt cgacttcaac cgtgaagtct
     2521 acgtgccctg gtgtatcgat gatattgact ctttcatctt tccatataca agtagtagca
     2581 gctgaagtta ttgttattcc tctttcctgc tcttgctcca tccagtccat ctgtgctgca
     2641 ccttcatggg tttctcctac cttatggatt ttaccggtat agaacaggat tctttcggtg
     2701 catgtagttt ttcctgcatc aatatgcgcc attattccaa tattcctggt attctctaaa
     2761 gaatattctc tcattttttc cctccttcac gctgcttttg ttttctgtct attaccatct
     2821 gaaatgggca aatgctttgt tagcgtctgc cattttatgt gtatcttctc ttttcttaac
     2881 actggctcca acgttgttgg ctgcatccat taattctttt gcaagcctct cacgcatagt
     2941 cttttctcct cttttgcgag aataagttac aagccatctc aatcctaaag tctgccttct
     3001 ttcaggtcta acttctatag gaacctgata agtggcaccg ccgactcttc ttgctttaac
     3061 ttccaacaca ggcatgatgt tgttcattgc tttattaaat atttccagtg gttcttcacc
     3121 ggtcttttct tttatgatat caaatgcacc ataaacgata ctttgagcaa ctcccttttt
     3181 accatcatac ataagctggt tgattaactt ggtaacagtc ttatcattat agataggatc
     3241 ttccattact tctctcttag gtatgtgtcc ttttcttggc actatgcttc cctccttaat
     3301 attaatttaa ttcacaggta ctcgacaatt aaatgccgtc gtgcacaatt acataatata
     3361 tattatggct tgtgccgaat atcatctaat ttattttttc ttttcccttt ttactccata
     3421 cttggatctt gcttgttttc tttttgctac accagctgta tcaagggttc ccctaataat
     3481 atggtatcta acccccggta agtctttaac tcttcctcct cttataagaa caacactatg
     3541 ctcctgcagg ttgtgtcctt ctccggggat atatgctgaa acctccatgc ctgttgttaa
     3601 ttttaccctg gcaatttttc ttaaagctga gttcggcttc tttggagtta cagtttttac
     3661 tgatgtgcat actcctcttt tttggggaga atttgattca ttcattctct tgttaagagt
     3721 gttatagctt acatttaatg ctggagaatc ggatttgtaa gtaacctgtt ctcttccttt
     3781 tctcactaac tggttaattg atggcattcg tacacctcct tttcttaaaa agttttattt
     3841 taaaacggca gcagccgctg cgtttacgcc gatacccaca gattttccaa gctctttcat
     3901 actatcgtaa tagattattt caatattctt ttccctgcaa acatctgtga ttgtcttagt
     3961 aacatgcaaa tcagcatcct ttgataaata aacaattttc gcttcgtctc tcgacaaggc
     4021 cctcaaagtc tgctttcttc caacaactgt ttttttgtca gtaaccattt caatcctcct
     4081 ttttctatga ctaatgtcat tctgaaggat atcacccttc agaatgacac actgcacaaa
     4141 actattggat taaaacactc aagtattcta ccatcatatt ttttagatgt caaataatat
     4201 tttatcctac atcagctgct ttttcaacat ctccggttgt tattcctata tttctgtatt
     4261 ttttcattcc tgtgcctgca ggtatcagct tgcctatgat gacattttct ttaagaccga
     4321 taagactgtc aacctttcct ttagtagctg catccgtaag aacccttgtg gtttcctgga
     4381 acgatgctga agccaggaat gaatctgttg caagagatga ctttgttata cccagaagtg
     4441 caacctcgcc tgttgcaggt tccttccctt catttacggc tgtttcattt tgctggttga
     4501 attcatagtt tgaaaccagg cttcccggaa gaagtgatgt atctccggaa tcctctattc
     4561 ttaccttatt caccatctgc cttatgataa cttcaacgtg tttgtcgctg atatcaacac
     4621 cctgcattct gtaaaccctt tggacttcca ttaagatata tgattccaaa gctttcatac
     4681 ccttaatgtt caaaatgtca tgaggattta ttgaaccgtc ggtaatcttg tctcctgctt
     4741 ctatataatc accttcgact accaaaattt ttgacccgta tggaatcaag taggtcttgc
     4801 tttccatcag ttcataatta gtaacggtaa cttctctttt gtgtgctttt tccgtcaagg
     4861 ttatctttcc ggggatttcc gaaattatag cctggccttt aggttttctt gcttcaaaca
     4921 gctcttcgat acggggcaga ccttgggtaa tatctgctgc cgccgcaata cctcccgtat
     4981 ggaatgtacg catagtaagc tgtgtgcccg gctcacctat tgactgtgca gcaattattc
     5041 caacagcggc tcccacgtta acaggctttc cggtagcaag atttgttccg tagcactttg
     5101 cacatacgcc atgaaccgtt ttacaagtaa gaacgcttct tacctttacc tttttaatat
     5161 cggcattttg aattttcaaa gctatatctt cagaaataaa atcgcctgct cttacatata
     5221 tttcaccggt ttcagggtct accacatcgt ctgcggcaaa tctgtcggcg catctttcgc
     5281 tcaatctttc aatttcttcc ttgccgtcaa taaaatcttc aacctctatg ccgtctgtgg
     5341 tgccgcagtc tgcttctctc actatgacat cctggcttac gtcaacaagt cttcttgtca
     5401 aatatcctga gtcagctgtt cttaatgcag tatccgttaa acccttacgg gcacctgtag
     5461 cagaaaggaa gaattccagc actgaaagcc cttcacggaa gtttgactta acaggaacct
     5521 caacagtctt gcctatggca cttgccataa gaccacgcat gcccgcaagc tgctttatct
     5581 ggttacgtcc tccacgggca cctgacacag acatgatgta gattggattc aagggactaa
     5641 ggttattcat aagagcattt gtaacatctt ccgtagtctg attccatact tttataacat
     5701 tgtcatatct ttcatcatca gtcatcaagc ctcttctgta cttcttttcg tattcgataa
     5761 cctgcttttc cgcctgctcg ataagagttt tcttttcttc gggaaccgta atatcgctta
     5821 ctgatatggt tattgcacct acagtagaat acctgaatcc cttttgcttg attccgtcta
     5881 aaacttcagc tgttctggaa ttcccgtgtt ttctgaagca gcggtaaatt atttcttcaa
     5941 gatatttctt ggtaacagtt ttatctattt caagtgagaa tttatccttt tccctgtcta
     6001 ctaatcccag gtcctgaggt atatcttcat taaatataaa tcttcccact gtggaatcaa
     6061 caagtcttgt ttcatcgccc acggttcttc tgaccttaac tcttgcgtgc aaatcaacgt
     6121 ttctgttgaa atatgccatc tttaactcgt cataatccgt gaaaatcatc ccctggcctt
     6181 tggaatcttc tatttccagc gtcatgaaat aggatccgag aaccatgtcc tgtgtaggag
     6241 tggttatagg tcttccatcc tttggtgcaa gaatattgtt cacggacatc atcagaaact
     6301 ttgattctga ctgtgcctca actgacaggg gcacgtgaac cgccatctgg tccccgtcaa
     6361 agtcagcatt gtaagccgta cataccagcg gatgaagttt aatagccttt ccttcgacaa
     6421 gcacaggttc aaaggattgt attcccagtc tgtgcagggt tggagcacgg ttaagcataa
     6481 cagtgtggtc cttgattatt tcatccagca catcccatac agccgtatca accttttcaa
     6541 cctttttctt tgcactcttg atgttgtggg cagctccggt ttcaaccagt ttcttcataa
     6601 caaagggctt gaacaattca agtgccattt tctttggaag gccacactgg ttaaacttaa
     6661 gctcaggccc tacaactata actgaacggc ctgaatagtc aacacgcttg cccagaaggt
     6721 tttgcctgaa acgtccctgc tttcctttaa gcatatcgga aagtgatttt aaaggtctgt
     6781 ttccgggacc tgttacaggt cttcctcgtc ttccgttatc tattaaagca tccactgctt
     6841 cctgcagcat tcttttttca tttcttacaa ttatatcagg agctcccaaa tcaaggagtc
     6901 tttttaaccg gttatttctg tttattaccc ttctgtacag atcgtttaaa tctgatgttg
     6961 caaaacggcc cccgtccagt tgaaccattg gtctcagttc cggcggaatg accggtatca
     7021 catccagtac catccattcg ggacggttgc cggaaaccct gaaagcttca attacttcaa
     7081 gccttcttat gattctaacc tttttttgtc ccttgctgtc cctcagctgg ctttttaatt
     7141 ctgctgcttc gctttcaagg tctatttttt caagaagctt tttaattgct tcagcaccca
     7201 tttcagcttt gaatgcattc ttatagtgtt ccttatactc tctgtattca atttctgtca
     7261 ataactgttt ttctgataaa gatgtatctc ctgcttcagt aacaacgtac tgggcaaaat
     7321 aaagtacctt ttccagtgct ctcggagaca tatcaagaat aaggcccatt cttgaaggaa
     7381 tacccttgaa ataccatatg tgggaaacgg gggcagcaag ctcaatgtgc cccattcttt
     7441 ctcttcttac ttttgccttt gtaacctcaa ctccgcagcg gtcacaaact acgcccttgt
     7501 atcttactct tttgtatttt ccgcaatgac attcccagtc ctttgtaggt ccgaatattt
     7561 tttcgcagaa aagcccgtcc ttttcaggtt ttagtgtcct gtaatttatt gtttcaggtt
     7621 ttttcacttc tcctcttgac cactgtctta ttttatcagg agaagccaaa ccaattctta
     7681 ttgattcaaa attattgtaa tctaccaagg agttcgctcc cttcttttat ttagtttttg
     7741 gtattcctta aaaatcttcg tcgtcataat cctcttcaag gtccaggtca tcaagttctt
     7801 cggaatcaat ttcaatatcc tcaatatcgt cgatatcttc ttcaatatcc tctacctcaa
     7861 cctgcatgcc ctcaaaatca tcttcaacaa aagtcatatc atttaaagct tctgtatcta
     7921 caggcaaatc atcatcattg tccatagttt caattatttc aacttcatca tcgctgttaa
     7981 taattttgat atccagggca agactctgca attcctttac aagcaccttg aaagattcag
     8041 ggattccggg ttcaggaata ttctcgcctt ttacaattgc ctcatatgtt tttaccctgc
     8101 cgttaacatc gtcggatttt accgttaata tttcctggag tgtgtgggct gctccatatg
     8161 cttccaacgc ccatacttcc atttccccaa acctttgacc gccgaactgg gctttacctc
     8221 ccaaaggctg ctgggtaacc aatgaatagg ggcctgttga acgggcatgt attttatcgt
     8281 cgacaaggtg gtgaagttta agcatataca tatatccaac agttacggga ccttcgaagt
     8341 attcccctga ccgcccgtct ctcaggtcaa ttttgccgct tcttgggtat cctgccaatt
     8401 cgagggcatc ccatatgtca tcttcaacag caccgtcaaa taccggcgtt gccacttccc
     8461 agcccaattt tttggcagct aatccaaggt ggacttccaa aacctgaccg atattcatac
     8521 gggaaggaac tccaagagga ttcagcacta cctgcagagg tgttccatcc ggcaggtaag
     8581 gcatatcctc ctcaggcatt atcatagaaa taacaccctt gtttccatgc cgtccgcaca
     8641 tcttatcgcc tacgttaatt ttacgttttg ttgctatata tactctgaca agcttgctta
     8701 caccggggct cagctcatcg ccattttccc ttgtaaacac ttttacatca acgattatac
     8761 cggtttcacc gtggggaact ttaagtgaag tgtctcttac ttctcttgcc ttctcgccga
     8821 atatggctct aagcagcctt tcttcagccg taagctccgt ttctcccttg ggtgtaacct
     8881 tgcccacaag aatatcaccg gatttaacct cggcgcctat tctaataatt cccctctcgt
     8941 ccaaatcttt gagagaatca tctcccacat tgggaatatc ccttgttatt tcttcaggac
     9001 caagcttggt atcccttgct tctgattcat attcctcaat atgaacagat gttaaaatat
     9061 cttccataac cagcttttca tttaagagca tggcatcttc gtagttataa ccctcccagg
     9121 tcataaatcc tattagaaga ttctttccta tagccatatc gccgtcatgg gtggaaggtc
     9181 cgtcagcaat aatctgacct ctttttattt catcgccttt ggatattata ggtctttgat
     9241 ttacacaggt accgctgttt gaccgcttga acttaagcag ggtatacttg tcaatcatgc
     9301 cgttatcgct tcttttgatt aaaatctgcg aggcagtgac agaaactgct gtgccgtcat
     9361 gttctgcaat aatcacagct cccgaatcca tggctgcctt gtgttccaaa cctgttccta
     9421 ttatgggaga ttcagtaata agcaggggca ctgcctgtct ctgcatgtta gcccccatca
     9481 gggcacggtt cgcgtcatca ttttccagga agggtatcat gcctgttcct atggaaataa
     9541 cctgttttgg agaaacctcc atataatcaa ccatgtcttt tgaagcaatt tcatttatac
     9601 cgtcaattcc cctgacaaca acacggctgt gttcgaaggt accgtccggg ttcaagggtt
     9661 cattggactg tgcgattata tttgtatctt ccacgtctgc agtaagataa tctataatat
     9721 ccgttacttt tcctgttttc ttatcaactc tcctgtatgg agattccaaa aatccataat
     9781 cattaattct tgcatatgtt gcaagagatg ttataaggcc tatattagga ccttcgggcg
     9841 tttcaattgg gcacatcctg ccgtaatgag aatggtgaac gtctcttact tcaaagcttg
     9901 ctctgtccct ggacaatccc cccggaccaa gagctgacat tcttctttta tgggttagct
     9961 cagccaatgg atttgtctgg tccataaact gggacagctg gcttgagccg aaaaactctt
    10021 tgatggctgc agacacaggc ctgatattta tcaaaacctg aggagttacc acatccacat
    10081 cctgaatagt cattctttcc cttactactc tctccatcct ggataatcca accctgacct
    10141 ggttttgcag aagctctccc actgacctaa gccttctatt tcccagatgg tcaatgtcat
    10201 cagtcttgcc tatgccttta aataatccaa aatgataatt taccgttgca tacatatcat
    10261 ccttgacaat atgttttggg gacaaggagt atacttcttc cccgtctttt tcaaaaatat
    10321 tgttatcagc agcagttatc tgttttaaag attcaattat tgctttttca atttcttctt
    10381 tagtcttgtt tacctccagt atttttttca tcaccggata ataaaccttc tcttttaccc
    10441 ctaatttttc taaatctata tttaattcat ctaacttaaa taccttcgga tcaacaaatt
    10501 tgttgcttaa tatcttagta accttatcat ctgtattaac aatattaact gaaaaaatgc
    10561 ctgctgcttc caatttaaaa attgaatcgg agtcgaatgt agtttctttg gcaaccaata
    10621 cttcacctgt ttccttatca attatatcat ctgcagcacg cttgccgata attctgtttt
    10681 ccagactaag cttcttgttt aatttgtatc tgcccacctt tgcaagatcg tacctcttaa
    10741 aatcaaaaaa caaggattta ataagtgact ttgcactgtc atatgtaggc ggctctccag
    10801 gcctcaatct tttataaatt tctatcagtg cttcttctga gttggtagtg ttgtcctttt
    10861 ccaaggtttt taaaagctgt tcggtttcgc ccaataaatt tattatctct gaattggagg
    10921 aaacatccat agcccttaca aggacagttg caggcagttt cctggttctg tcaactctta
    10981 catagacaat atcattggag tctgtttcaa attccagcca tgctcccctg ttaggaataa
    11041 ctgttgcatc atatagttcc ttgcctacct tatccctggt catgttgtaa taaacgcccg
    11101 gggaccgtac caactggctg actacaacac gctcagcccc gtttataatg aatgttccct
    11161 tttctgtcat cagtgggaat tcacccataa aggcttcttg ttccttaatt tctcccgtct
    11221 ccttgtttat cagcctgacc ttgagtttta gaggcaccga gtatgtagca tctctttcct
    11281 tgcactccac ttgattaaac ttgggttcgt ttaataaaga gtggtcaaca aactcaagaa
    11341 ttaaattgcc ggtataatcc tcaatcggag aaatatcatc aaaaacctct ttcaatcctt
    11401 catttaggaa ccaattgtat gattttttct gaacttcgat taagtccggc aattctaaaa
    11461 cttcattaat tttcgagtaa gacattctta ccctattacc tattttcaca ggatgcggca
    11521 ttttttcacc ccttagttaa taattttgac ttacaaaatc tgtgccaaca gaacacacta
    11581 atggcaatat acaataatat cactattaaa attttatgtc aacataaatt tttacgattt
    11641 tattgttatt ttttgccatt atcctacatt tatcgagttg ttttttttgc cagtcaatta
    11701 aaaaatcaga aaacgccttc attattattt gcattatttt ggaaaatata catatataat
    11761 tttggtcata caataaaaac ataaatccca tcggcagcgg gtccccagtc tcaatcacca
    11821 aaacatctgc ttcctttggt tagggcatga aaaaagactt tacaaaagtg taaagtctcc
    11881 tggaaagtag aagggggaca cacctctatc attaagaaag tcttcgctgg catgaggaat
    11941 gtccccgttc ttgttattat ttaatttcta ctgatgcgcc ttcagcttcc aatgctgctt
    12001 ttgctttttc ggcttcttct tttggaacgg ctgtctttat tgttgtagga gctccatcaa
    12061 ctgcatcttt agcttccttc aagccaagtc ctgttaattc tcttactact ttaattacct
    12121 tgatttttcc tgcgccagct gctgtaagaa caacatcaaa ttctgtcttt tcttcttcaa
    12181 caggagctgc tgcacctgct gctgcacctg caactgctac aggagctgct gctgatacgc
    12241 cgaattcttc ttctattgct tttaccaatt cgtttaattc taatactgat aacttcttga
    12301 tttcttcaac gaagtttgtg attttttcac ttgccattat attttcctcc tattaatttg
    12361 tggattaatt ttttaattta attattattt ttgactcatt ttgtagtctc gcagtctcat
    12421 tcaccaaaat atttgctcct tttagtcaca tattttggga attcgttgcg aatgacctac
    12481 catcaatttt tctgaaaaac cactctgaaa aattgggtta gggcatctta tgctgcttcc
    12541 tcgccttttt tgcttatctg gtcaagcata taagcaagat ttctgatatt tccctgcaga
    12601 acgcccgcca gctgtccaag aagtatgtct ctggacggta cgcttgccaa ttctttaact
    12661 tcatcggcat ttaaaatttt gccttcggct attccggctt ttaatattaa tgtatcattg
    12721 ctgtctttag caaaatcatt agttaccttt gcggctgaaa cttcatcacc ataagacaag
    12781 gcaatggcat tggggccttc caggttaacc gacataccat tatatccaag ctcttcaaat
    12841 gcaaaacgca ttaaagtatt cttatagact ttatattcaa ctcctgcttc tctgcacttg
    12901 tttcttaata aagtagcctt ttcaactgtt aatcccttat actcaactaa cacaactgat
    12961 ttggcatttc ttattttttc agtaatctca cttactacct gttttttctg ttctaatact
    13021 gattgattca ctttgttgca cctccttttg tagaaagtct tttcttttta ctcgcatcac
    13081 gaaacctatt gtgagactgg taacgcaggg gaattcacca aattttttgt tcgctttggc
    13141 tcacaaaatt ttggaattct ttgcgtttga attacctacc attttttctt cgcttctctc
    13201 gaaaaaatgg ggtaaatcat aaaaaattct ctccgcagac gaagagaaag aatctattat
    13261 ctcacctcgg ccgggtattt aaactcatca ggagtaccgg ctgtctgcgg tattatattc
    13321 aattttggtt gttgcactat tccgctttcg ttttcgatac gaacgaatct gttaacttag
    13381 ttgtattaag cttgattcca ggccccattg tgcttgcaat agtaacactt tttaagtatt
    13441 ttcccttggc ggctgccggt ttggctttta aaaccgcatc aattataacc tttaagttgt
    13501 caacaatttt ttctgtaccg aatgatttct ttcctgcagg aacgtggata atgtttgttt
    13561 tgtcaagtct gtattcaacc ttacctgctt taatttcttg aagagccttt tctacatcaa
    13621 atgtaactgt acctgattta gggttgggca tcaatccttt aggtcctaaa acccttccaa
    13681 gctttccaac tacacccatc atatccggtg ttgcaataac aacatcgaaa tcaaaccagt
    13741 tttccttttg aattttttct gccagttcac cttcacccac aaagtctgct cctgcttgct
    13801 gagcttcttt agccttgtcg cctttggcaa aaaccaatac ccttacagtt tttcctgttc
    13861 cgtggggaag aactacggca cctcttacct gttgatctgc atgtcttgaa tccacgccca
    13921 attttatatg aacttccact gtttcatcaa aattagcctt tgctgttgat agcatagctt
    13981 ccacagcttc atccatatcg tataatttca gtttttcaat tgactttata ctgtcttgat
    14041 atttctttcc tctttttggc atattgtatt cctccctgtg gtcaagcagc cttatatcgg
    14101 cctcccactg ccatcttgtt attcttccac ttttactccc atgcttcttg cagtacctgc
    14161 aaccagactc atagctgctt ctacagtgtt agcatttaaa tcaggcattt ttaactttgc
    14221 tatttcttcg cattttgcct ttgaaagtgt tgcaaccttc tttttgtttg gttctcctga
    14281 accgctttca attccaactg cctttttgat tagaactgca accggcggag tcttcgtgat
    14341 aaatgtaaat gatctatcct ggtatacagt caacacaacc ggtataatca tacctgcctg
    14401 atcggctgtc tttgcattaa actctttaca aaatcccata atattcacgc catgaggtcc
    14461 taatgccgta cctaccggtg gagccggagt agctttacct gcgggtatct gcagttttac
    14521 aacagctgtt actttttttg ccatatttcc acctccttca tcgcataacg atgttatatt
    14581 gaaaagtaat tatactttct caatctgatc atattcaagc tcgataagag tttctctacc
    14641 gaacattgat acaaaagctt taatttttct tttttcctga ctgatactat ccacagtacc
    14701 gatgaaatct tcaaaaggtc cttcctttac tttgattgta tctcctgctt caagttcaat
    14761 tgccggaagt ttttcaacta cgcccagcgc ctttacttct tcatcagtca gcggaacagg
    14821 ttttgatccg ggacctacga agcctgttac tccccttgtg tttctgacaa gataccaaga
    14881 ttcatcggtc attatcattt taataattac ataactgggg aacatttttc tttctttgat
    14941 ttttttcttg ccgtttttta cttccgctac ttcctctacc ggcactctca cttcaaatat
    15001 cgtatcctgc atctctctgt tttcgacaag cttttcgata tttgccttta ctttattttc
    15061 atgcccagaa tatgtatgaa ccacatacca tcttgatcct tgcgactctt gcttcatacc
    15121 ttactccttt taaacaatca cagtaagcaa acccctgaat atcaaatcca gtccccagat
    15181 tacaaccgtt aatgcaaatg tagtagctaa aacaacaatt gtataatttt gtaattcttt
    15241 tctggtaggc cagtttactt ttttcatctc tgaccttacg cctttaaaat aatttttaac
    15301 ccttgttaga aatttcggtt tttcattatc cctgttagac attcccacac ctcgttattt
    15361 tgtttccctg tgaagagtat gttttttgca gaacttgcag tacttgttca tttcaattct
    15421 ctcaggattt tctttcttgt ttttttctgt atcataattt ctttgtttgc actcagtgca
    15481 tgccaactta actgccactc tcatttttta cacctccaag aacttattcg cataagaacc
    15541 cattatctta ctcaagatat cacaaatcaa attaactgtc aatattttat atattttttt
    15601 tattcaaata attcattgaa attatcatga atttcaacag ttatttgctt gtttcactaa
    15661 aaaaagctcc tgcccaagca aaagcttttc ttattcagca attaatttta caacaacaat
    15721 tcttacttgt caacaatatt ttttggggga tggttctttt tgttccaaga acatgatgtc
    15781 acccctgtcc ccttgccatt ctgttgtaaa aataataaaa ataatgagcg aacatttgca
    15841 gggcacgatt gtaattctta gcgaaggtcg gttaaaaatc cgttaagcag attgcactgt
    15901 ttgagcgaag cgagtttgca agctgcagga tttttagcca taatgaactt tagaattacc
    15961 cgtgtccgaa actgtgagcg gttattttta ttattttttt gtaaccggga gcagttattg
    16021 ttattatt
//
LOCUS       DAOPUT010000032        15621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3840_length_15621_cov_19.549981, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000032 DAOPUT010000000
VERSION     DAOPUT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15621
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3840_length_15621_cov_19.549981"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKK75_04365"
     CDS             complement(<1..362)
                     /locus_tag="PKK75_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNZ82861.1"
                     /translation="MKGFLEKIFGSYSEKEIKRIQPTVDKILSLEKTVEALTDSQLKA
                     KTQEFRQRLAEGESKDDILPEAFAVVREAAWRVLGQKHYPVQILGGIVLHQGRIAEMK
                     TGEGKTLVSTLPVYLNAL"
     gene            complement(380..1600)
                     /locus_tag="PKK75_04370"
     CDS             complement(380..1600)
                     /locus_tag="PKK75_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ82862.1"
                     /translation="MKIAVIGGGASGLTAAIAAAKNGAEVTIYEKLNRVGKKILATGN
                     GRCNYTNMNLSKECYHSKDLKLTEDVMNFFDLKKTLDFFENLGIFPYVDDSGKVFPNS
                     LQASSVLDVLRYELQRLKVKELTDFNVTDLRKTKDKFSVIGNDTHAADRIILSTGGKA
                     SPQLGSDGQGYEIARSFSHEIIEPFPALVQLRLSGKYFKRMAGIKFDGIVKAYAGERL
                     IREEEGEILFTDYGISGPPILQVSRRVIEELNKKNKPFLTVDMLPLYSKLKLYEILEE
                     RFRKIDYKTIEEGMVGFINKKLIPVVLYEAGFDDINKLCGRLNKKEIYKIIDILKEWK
                     FEVTGHNSWQQAQSTAGGIKLSEVNPKTLESLKVKGLYFAGEILDVDGDCGGFNLQWA
                     WSSGYTAGYFSSLK"
     gene            complement(1604..3205)
                     /locus_tag="PKK75_04375"
     CDS             complement(1604..3205)
                     /locus_tag="PKK75_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82863.1"
                     /translation="MYRIQQIKLPIDHKEEELLIKAAEALRIKKEDIKEVTIFRKSID
                     ARKKDEILFTYSLDVDTYKEVKISKRNGNISIAEPFVYDVQATGEKPLNNRPVVVGSG
                     PAGLFCALLLAEKGYRPIILERGKTVKERVKDIEKFWKEGILNTGSNVQFGEGGAGTF
                     SDGKLNTLIKDRSKRGKKVLEEFVAAGAPAEITFINKPHIGTDLLRNVLVNIRKKIIS
                     LGGTFRFEECLTDIIIRDNAVCRIVTESDIIETDVLVLAIGHSARDTFEMLSNKIHLT
                     SKPFSIGVRVEHPQKMISEAQYGDMAGNPLLGPADYKFVHKCKNGRSVYTFCMCPGGL
                     VTASASEAEGVVTNGMSFHERDLENANSAVVVQINPEDFGGDENPLAGIEFQRYWERK
                     AYQAGGGNYHAPVQLFKDFKEKTVSSSFGEIYPTYRPGCTFANLWDCLPDYVCESLAE
                     GITAFSKLLANYDRPDALLTGVETRTSSPLRITRDDEMQANIRGIYPCGEGSGYAGGI
                     MSASIDGLKAAEQIIKTYKSIKEQE"
     gene            complement(3289..3714)
                     /locus_tag="PKK75_04380"
     CDS             complement(3289..3714)
                     /locus_tag="PKK75_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNZ82864.1"
                     /translation="MDQTDLRIISILQHDGRISMKELGKKVSLSPPAVAERVKKLEEK
                     GVIERYKAVINSTKIGKPISVLINASIKPENQEEFLKFARSSDEIVECHHVTGPHSMI
                     MRAYLREMTHLEELVRKIQSFGNTETYVIMSSPIDDLTI"
     gene            4046..5416
                     /locus_tag="PKK75_04385"
     CDS             4046..5416
                     /locus_tag="PKK75_04385"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HNZ82865.1"
                     /translation="MTKLKITETSLRDGHQSLIATRLTTDEILPVLSEMDNAGYHSLE
                     VWGGATFDTCLRYLNEDPWERLRKIRANVKNTKLQMLSRGQNILGYKNYPDDIVERFL
                     KNAIYEGINIVRVFDALNDIRNLRKSIEVVKSEGAHCQCAISYTTSPVHTKDYYLKLI
                     KDFEGAGADSICIKDMSGILLPIDAYHLVKAIKEVTNLPLELHNHCTGGIAEMTVLTA
                     VEAGIDIVDTAISPLSSGTSQPATESLCLTLKDTNRDPKLDMQSLNEIADYFKYVMKK
                     YEEEGTLNVKVLMTEPKALIYQIPGGMLSNLLIQMKSLNAGDKFQEVLDEVPKVRKDL
                     GYPPLVTPLSQMVGAQAVFNVISGERYRTIPTEIRNYVRGLYGMPAASIPDEIKKLII
                     GDEEVITVRPADLLEDSYERLKQEIGYLAQSEQDVLSYALFPQVAKCFFETRNQKVLV
                     GHWGRS"
     gene            complement(5441..6190)
                     /locus_tag="PKK75_04390"
     CDS             complement(5441..6190)
                     /locus_tag="PKK75_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ82866.1"
                     /translation="MPRFARRLSETRIYHVIFRGVSRYNIFEEEKDYQKILEIITDLK
                     EEMDFDILAYCLMSNHAHLIIRELELGEISLIMKRMLVRYAGWFNKKYDRSGILTSNR
                     FSSEPVETDDYLITLLRYIHQNPVKGGLASTIESYEWSSFKEYCGKGQLCNTDIVLSI
                     IDRDSFINFNYEIEEDEYEISMKAIKIDDEFVRLRIKEILGGKDPFELGEMPINERNK
                     IIKQLKNVERFSIRQIERVTGISRGVISRCK"
     gene            complement(6548..8275)
                     /locus_tag="PKK75_04395"
     CDS             complement(6548..8275)
                     /locus_tag="PKK75_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82867.1"
                     /translation="MNNTQDNKRALNIIWNASGDYSIDPQFKAYDENGEADIYWNYII
                     GAVHKYYDYPLLRGFFNYLKTDSEHEFYEKLFWLGLEKSVFDKGKYHRPVLNSLRKSH
                     ARKVLAGEAETFDDNLLNVIRNAHFRKALGEPPGITGNLLNILNDLEFGDAETEEIIS
                     KMNWIIKTYFRFNYGRYEVNTETNKKKVQKQVQQSDEEKLSDSDMEAMKDLYLDSAET
                     SREVYYEQEKEKSKKPVRKVRLENKDDSERQFIRKYFGASIISQQKTYLIERGLCVGN
                     HKNTHLHFTRGEFDDEFGDDTNALYYNKILKEQRQINLDNFNANYIKYKNSILKLTNK
                     IRNTMLAYFESYTGKAEAGRLNPPVIWRNIYLNDNRVFTKVYNNDMANLSVDILLDAS
                     ASQHERQTTISAEAYIIAESFTQCQIPVRVYSFSSLRNFTVINLYRDYDESDNMQIFN
                     YKTTGCNRDGLAIRTALYMMESSNYFNKLLIVLSDCKPNDTQCIPDTGIKQEQTKYAG
                     ATGIIDTAFEVKKGINNGNSILCVFTGEDEDIPTAKKIYGHNFARIYYPERFAEIVGV
                     LMQNQLKNL"
     gene            complement(8278..>9172)
                     /locus_tag="PKK75_04400"
     CDS             complement(8278..>9172)
                     /locus_tag="PKK75_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNZ82868.1"
                     /translation="KEGIKERLINDIEKFRTFYKVDPKLEYRIPRPKYNYYGSDVWEE
                     AITALLCGENLLLVGSKATGKNVLAENLSAVFGRPKWDISFHVNTDSTSLIGTDTFKN
                     GQVVFRNGPIYECAKWGGFGVLDEINMAKNDSVAVLHATLDYRRIIDVPGYDKITIED
                     ETRFIATMNYGYAGTRELNEALTSRFMVINMPNISKENLKKLLQREYPGLRDEYIEQF
                     AGLFQDLQLKSENSEISTKSVDLRGLLASINLMDKGLSPAKALQMGIANKSFDDFERT
                     LVEDVIRLRIADNLSRNEIFR"
     assembly_gap    9173..9272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9489..10847)
                     /gene="radA"
                     /locus_tag="PKK75_04405"
     CDS             complement(9489..10847)
                     /gene="radA"
                     /locus_tag="PKK75_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNZ82869.1"
                     /translation="MAKLKSKFVCQECGYETAKWLGKCPSCEEWNTFAEEFESKKTDA
                     KSQRGISKGMVEKLNNITPAKKERISTGSMEMDRVLGGGIIKSSLILVGGDPGIGKST
                     LLLQVADHAAKQNLKVLYVSGEESGEQIRIRADRLGIGDGDLYVLAETNIDIIIEYVE
                     KEEPDLLVLDSIQTIYSPDVASAPGSVTQVREVTSMVMRMTKTRNMTSFIVGHVTKSG
                     AIAGPRVLEHMVDTVLYFEGERHFSYRILRAVKNRFGSTNEIGIFEMRDRGLVEVENP
                     SEVFLKGRPLDAYGTVVSAAMEGTRPVLIEIQALVTYSPVGFASRVTTGIDKNRVSML
                     IAVLEKKAGLSIQSSDTFINVTGGLQIKEPACDLAVLCALASSFKEIPVDFKTVLIGE
                     VGLTGEIRGVNSIEKRLLEANKMGFERAVIAEANAEISKEIKNMEIIAVNNIRQVMDI
                     LF"
     assembly_gap    11093..11192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11315..11851
                     /locus_tag="PKK75_04410"
     CDS             11315..11851
                     /locus_tag="PKK75_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ82870.1"
                     /translation="MNIGENLKRIRVANSLTQQEVADRCELTKGYISQLERDLTSPSI
                     ATLTDILECLGTDLAGFFNEAVDNKIVFKHDDVFVKETEDNYVINWIVPNAQKYKMEP
                     ILVELGEDGRTKADEPHEGEEFGYVLNGSILLNYGSQVYKVKKGESFYIKTNKTHYIE
                     NAGKSTARVLWISTPPYF"
     gene            11863..12903
                     /locus_tag="PKK75_04415"
     CDS             11863..12903
                     /locus_tag="PKK75_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82871.1"
                     /translation="MDNAIIKLNNITKKFDGETVLDDITLSVKENEFVTLLGPSGCGK
                     TTTLRIIGGFETPDEGEVYFDGQLINDIPPFKRQLNTVFQKYALFPHLDVYENIAFGL
                     RIKKMDEAAITAKVEKMLELFNLKNYGKRRIDQLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRKEMQHELKAMQKQLGITFIYVTHDQEEALTMSDTIVVMNEGIIQQMG
                     TPIDIYNEPENAFVADFIGESNILDGVMIDDYLVEFLGKRFECLDAGFGRGEEVDVVI
                     RPEDLDLTPVGQGMLEGIVRSITFKGVHYEMDIAANGFTFLVHSTDMAPVGATVGLTL
                     TPDDIHIMEKGE"
     gene            12942..13748
                     /locus_tag="PKK75_04420"
     CDS             12942..13748
                     /locus_tag="PKK75_04420"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82872.1"
                     /translation="MVWIIIFIALPTFLVLLYSITTKESNGLTTIQFTSENFRKFFQP
                     IYLDILWDSIYLAAVSTAICLLLGYPAAYIISNTHISRRNTLLFLCILPMWMNMLLRT
                     YAWMTILGNNGILNNFFAFLGLPRFNMLYTRGATVMGMVYNFIPFMILPIYTALSKMD
                     KGLLEAADDLGADKKTVFRKVILPLSMPGVISGVIMVFMPAVSTFIIPQLLGGNKSMM
                     IGNLVEKLFILNGDWNFGSAISIVMMLIILLSMSIMNKFDVDKEGGARLW"
     gene            13742..14548
                     /locus_tag="PKK75_04425"
     CDS             13742..14548
                     /locus_tag="PKK75_04425"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ82873.1"
                     /translation="MVKKYLSKIYMGIIFIFLYAPIIVLAVFSFNESKSRGNWTGFSL
                     KWYIELFHDHDIRLAFYYTISIAIITAIVSTIMGTIAAIGINAMKGRQKALILNVNYL
                     PVVNPDIVTGISLMILYISFNMDFGFNTMLISHIVFSIPYVILSILPKLKQLDVHMTE
                     AAMDLGATPMYALRKVILPEIKPGIITGFLMAFTLSIDDFIISYFTKGEGVTNLSIVI
                     YSMARRGVRPTINALSTIMLTVVLLLMILINKRNNGQDKSKKINQSEVYK"
     gene            14545..15585
                     /locus_tag="PKK75_04430"
     CDS             14545..15585
                     /locus_tag="PKK75_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ82874.1"
                     /translation="MKSKLILFVSILCLLLIAATGCSTEEKQTLKVLNYDIYIDKSLL
                     SEFEKANNVTIKYDTYSTPEDMYIKVKSGASKYDLIISTEYMIERMISENLVNKLNFD
                     NIPNYKYISEDFKNHVYDPNNEYSVPYFWGTLGILYNKTTVDASSDSWAMLWDKAHAQ
                     RIIMMDSQRDSFAAALKLLGYSMNTVDERELDEAKALLIEQKPLVMAYITDGSPAIMV
                     NEEADMALVWSGEAVAAMSENENLDFVIPKEGSNIWIDAMVIPSTTENQELAEKFINF
                     LCTTEATLRNIDEVWYSTVHTEALEQVDEELLNNPAFNIPREDIVNMEMLRDPKEFIN
                     LYSERWTELMAE"
BASE COUNT         4392 a         3082 c         2851 g         5096 t
ORIGIN      
        1 tcaagggcat tcaaataaac aggcagggtt gaaaccagtg tttttccttc tcctgttttc
       61 atttcggcaa ttctaccctg gtgaagaact attccgccta atatctgtac agggtaatgc
      121 ttctgcccca gtacccgcca ggcggcttct ctgacaacag caaatgcttc aggcagaata
      181 tcatcctttg actcgccctc tgcaagtctt tgtctgaatt cctgagtttt agccttaagc
      241 tggctgtctg tcagggcttc aaccgtcttt tccaatgaca atattttgtc aactgttggc
      301 tgaattcttt ttatttcttt ttcactgtat gaaccaaaaa ttttttccaa aaaacccttc
      361 atttttttat ccttccactt tattttagag agctgaaata tccggctgta tagcctgagc
      421 tccatgccca ctgcagatta aatcccccgc agtctccgtc cacgtcaagg atttctcctg
      481 caaaatacaa gccctttacc ttcaaagact ctaaggtttt tggattaacc tccgaaagtt
      541 taatccctcc tgccgttgac tgggcttgct gccatgaatt atgtcctgta acctcaaatt
      601 tccactcttt taaaatatct attattttat atatttcttt cttgtttaat ctgccgcata
      661 atttatttat atcatcaaaa cctgcttcat ataatacaac aggaattaat tttttattga
      721 tgaagccaac catgccctct tctattgtct tatagtcaat ttttctgaat ctttcttcca
      781 gtatctcata gagcttaagc tttgaataaa gaggaagcat atctacagtt aaaaagggct
      841 tgtttttctt gtttaattct tcaattaccc ttcggcttac ctggagaatt ggaggtcctg
      901 aaatgccgta gtcggtaaaa agtatttccc cctcttcttc ccttatgagc ctttccccag
      961 cataggcttt tacgatgccg tcaaatttta ttcctgccat ccgtttaaaa tattttccgc
     1021 ttagccttaa ctgaacaagg gcaggaaaag gctcaattat ttcatggctg aaggacctgg
     1081 ctatttcata tccttgtccg tcagagccga gctggggact tgcttttcct cctgttgaaa
     1141 ggataatcct gtctgcagca tgagtatcat ttccaataac agaaaattta tctttggttt
     1201 ttcttaaatc tgttacgtta aaatcagtaa gttccttaac cttaagcctt tgtaattcgt
     1261 atcttaaaac atctaaaaca cttgaagcct gaagagaatt cggaaaaacc ttcccgctgt
     1321 catccacata aggaaaaatt cccaggtttt caaaaaagtc aagggttttt ttaaggtcaa
     1381 aaaaattcat tacatcctct gtaagtttta aatcctttga atggtagcat tcctttgaca
     1441 ggttcatgtt tgtataattg cacctgccgt tgccggttgc aagaattttt ttgcctactc
     1501 tgtttaactt ctcgtaaatg gttacttctg caccgttttt tgctgcagct attgctgctg
     1561 tcaagcctga tgctcctcct ccgattacag ctattttcat aatttattcc tgttccttga
     1621 tagacttgta tgttttaata atttgctctg ctgccttcag gccgtcaatt gatgctgaca
     1681 taattcctcc ggcatagccg gacccttcgc cgcaggggta aatacccctg atatttgcct
     1741 gcatttcatc atctctggtt attcttaacg gggatgatgt tctcgtttca actccggtaa
     1801 gaagtgcatc cggcctgtcg taatttgcta aaagcttgct gaaggctgtt atcccctcag
     1861 ccaggctttc gcatacatag tcaggcaggc agtcccacag gtttgcaaag gtgcagcccg
     1921 gtctgtatgt tggataaatt tctccaaagg atgaagaaac ggtcttttcc ttaaaatcct
     1981 taaaaagctg tacaggtgcg tggtaatttc ctccccctgc ctgataggct tttctttccc
     2041 agtatctctg aaattcaatt cctgccaggg ggttttcatc tcctccgaaa tcttccggat
     2101 taatctggac aactacggca gaatttgcgt tttccaaatc tctttcgtga aaagacatgc
     2161 cgtttgtcac tactccctca gcttcggaag cggatgcggt aacaagacct ccggggcaca
     2221 tgcagaatgt atacacgctt cttccatttt tgcatttatg aacaaattta tagtcagcag
     2281 gtcctaaaag cggattgcct gccatatctc cgtattgagc ttcacttatc attttctggg
     2341 ggtgttcaac cctgacacct atggaaaatg gttttgatgt aagatggatt ttattgctga
     2401 gcatttcaaa agtgtctctt gcactgtgac ctattgcaag aaccaataca tctgtttcaa
     2461 tgatgtcgct ttcggttaca attctgcaga ctgcattatc tctaattatt atgtctgtca
     2521 aacactcttc aaatctgaat gttcccccca gacttattat tttttttctt atattcacaa
     2581 gaacatttct taaaaggtct gttcctatgt ggggcttgtt aataaaggtg atttccgccg
     2641 gggcacctgc tgcgacaaat tcttccaaca cttttttgcc tctttttgac ctgtccttta
     2701 tcaatgtgtt aagtttcccg tcagaaaatg ttcctgcccc gccttccccg aactgtacgt
     2761 ttgagccggt atttaatatg ccttccttcc agaacttttc aatatccttc actctttcct
     2821 taacagtctt tcctctttca agaattatgg gtctgtagcc tttttctgca agaagcaagg
     2881 cacaaaaaag ccctgcggga ccgcttccta ccactacagg cctgttgttt aaaggctttt
     2941 ctcctgttgc ctgcacatcg tatacaaagg gctctgcaat agatatattg ccgtttcttt
     3001 tggatatttt cacttccttg tatgtatcaa catctagtga ataagtaaac agtatttcgt
     3061 ctttttttct ggcatctatt gacttcctga aaatggtgac ttcttttata tcttctttct
     3121 taattcttaa ggcttcagca gctttaatta aaagctcttc ctccttatga tctataggca
     3181 gctttatttg ctgaattcta tacattgtta tacctcttaa tttctgtcat tgaatataat
     3241 gtcccttaac taaaatattc atcagcccgg tccgaaggtt tggtgtgttt aaatggtcag
     3301 gtcatctatg ggactggaca ttattacata agtttccgtg tttccaaatg attggatttt
     3361 tcttaccaat tcctccaaat gggtcatttc ccttaaatat gctctcatta tcatgctatg
     3421 gggacctgta acatgatgac attcaactat ttcatctgag ctgcgggcaa atttcaaaaa
     3481 ttcctcctga ttctcaggtt ttattgatgc atttatcaat acgctgattg gctttcctat
     3541 ttttgtgctg tttattacag ccttgtagcg ttcaattact cctttttcct ccagcttttt
     3601 tactctctct gccactgcag gagggcttaa ggatactttc tttcctagtt ctttcatgga
     3661 aattcttccg tcatgctgca atatgctgat aattctcaaa tccgtctggt ccatcctaca
     3721 ccgccttata aaattaagta tatttacctt tttttattaa tttacaattg cgataatgtc
     3781 tttttatatt ataaacaaat caattataac atattattgt atgcattaat atttaaaaca
     3841 ggatatttta aaaaataata tatacaaaca ttattatttg tggtaatata ctttcatagt
     3901 caaactattt taatatcaaa ttaatattca atgttgatta ttaataaatt atatcatagt
     3961 tgctctaatc atatcaagaa aaaaaacatt cctgttctta tgaggataaa taaaataaga
     4021 cattattaag taaaaaggag aaaatatgac gaagttaaaa attacggaaa cctctctaag
     4081 agatggtcat caatcattga tagcaacaag acttacaact gacgaaatac ttcccgtatt
     4141 gtcggaaatg gataatgcag ggtatcattc cctggaggtg tggggagggg caacatttga
     4201 cacctgcctc agatacttaa atgaagaccc ttgggaaaga ctaaggaaaa taagggcgaa
     4261 tgttaaaaac accaagctac aaatgctatc acggggccag aatatattgg gatataaaaa
     4321 ttatcccgat gacatagttg aaagatttct aaaaaatgca atatatgaag gaattaacat
     4381 agtcagagta tttgatgcct taaatgatat aagaaatttg agaaagtcca tagaggttgt
     4441 aaaatcggaa ggagcccact gccagtgtgc aatatcctac accacaagcc ctgtacatac
     4501 aaaggattat tatcttaaat taataaagga ttttgaaggt gcaggagccg actcaatctg
     4561 catcaaggat atgtcaggaa tactgcttcc tatcgatgct tatcatttgg ttaaagctat
     4621 taaagaagtt acaaatctac ccttggagct tcataaccac tgcacgggag gaatcgctga
     4681 aatgactgta ttgacagcag tggaagcagg gattgatatt gttgacactg ccatatcacc
     4741 cttgtcttcg ggaacctcgc agcctgcaac tgaatctcta tgtttaaccc tcaaagatac
     4801 taacagggat cctaagcttg atatgcagtc tttaaatgaa attgcagatt attttaaata
     4861 tgttatgaaa aagtatgaag aggaaggaac tttaaatgtc aaggtgctta tgacggagcc
     4921 gaaagctttg atatatcaaa taccgggggg catgctttca aaccttctta tacaaatgaa
     4981 gtctctaaat gcaggagata aattccagga agtactggat gaagttccaa aggtaagaaa
     5041 agacctgggc tatcctccgc tggttactcc attaagccag atggtaggag cgcaggcagt
     5101 ctttaatgta atttcgggag aaaggtacag gacaataccc acagaaataa gaaactatgt
     5161 aagaggattg tacgggatgc cggcggcctc cataccggat gaaattaaaa agcttattat
     5221 cggagatgaa gaggtgataa ctgtgcggcc tgcagatctt ttggaggaca gctatgagcg
     5281 tcttaagcaa gaaataggat atcttgccca gtcagagcag gatgttttat cctatgcgct
     5341 tttcccccag gttgcaaagt gcttcttcga aacaagaaac caaaaggtct tagtgggaca
     5401 ctggggacgg tcctaaactg tcccactttc tttcattccc tcatttacat ctcgatatta
     5461 caccgcggga gattccggtg acccgctcta tttgtcttat agaaaatctt tcgacatttt
     5521 tcagctgctt gattatttta tttctttcat taatcggcat ttcacccaat tcaaaagggt
     5581 ctttaccacc caatatttct tttattcgca atcttacaaa ttcatcatct atttttattg
     5641 ctttcatcga tatttcatat tcgtcttctt ctatttcata gttgaaattt ataaagctat
     5701 ctctatctat aattgacaat actatatcgg tattgcacaa ctgacctttt ccacaatatt
     5761 ctttaaaact actccattca taactttcta ttgttgatgc cagaccgcct ttgactggat
     5821 tttgatgtat gtatctcaat aatgtaatta aataatcatc cgtctcaact ggctcgctac
     5881 taaatctatt agaagttaat atgccgctcc gatcatactt tttattaaac catcccgcat
     5941 aacgtacaag catccttttc ataattagtg atatctctcc taattctaat tctcttatta
     6001 ttaggtgagc gtgattgctc attaagcaat atgcaagaat gtcaaagtcc atttcttcct
     6061 ttaaatcagt aatgatttct aagatcttct gataatcttt ctcttcctca aaaatattat
     6121 atctgcttac tcctctgaaa attacatggt atattctggt ctcacttaac cttctagcaa
     6181 atcttggcat aagatcaccc gctttttgtt tttattatac attgccttcc atttaatgtc
     6241 aaaacattca agaaataaca tacgcatatt ttaataataa ttagtgaaaa ttattccaga
     6301 ataagacaat aaaatagtac ttattggtct gccctcattg cggagaaaag cgtaaaaaat
     6361 tattctaaat gtgaacttcc aaaaaaatag tacttaattg aaaatcatat tcaaaaacaa
     6421 agaaagatcc acaagataga caatttccac ttgattgtaa gctattcttg ttatacttat
     6481 tgagtaatta caatactcaa caatgagtcg gaaagtgaga cagtttagga ccgtccccag
     6541 tgtcccgcta taggtttttg agctggtttt gcataaggac ccctacaatt tctgcaaaac
     6601 gttccggata atatatacgg gcaaaattgt gtccgtatat tttttttgct gtaggtatat
     6661 cttcatcttc tcctgtgaaa acgcaaagaa ttgaattgcc gttatttatg ccttttttaa
     6721 cctcaaaggc agtgtctatt atgccggtgg ctcctgcata cttggtctgt tcctgcttta
     6781 tccccgtgtc gggaatgcac tgggtgtcat tgggcttgca gtcggaaaga actatcagga
     6841 gcttgttgaa ataattgctg ctttccatca tgtacagggc tgtgcggatg gcaagtccgt
     6901 cccggttgca gcctgttgtt ttataattga aaatttgcat gttgtcgctt tcatcgtaat
     6961 cgcggtacaa atttataact gtgaagtttc tcaggcttga aaatgaatag actcttaccg
     7021 gaatttggca ttgggtaaag ctttcagcaa taatgtaagc ttcagccgaa attgttgtct
     7081 gtctttcatg ctgggatgcg gatgcatcaa gcaaaatatc cacagacagg tttgccatat
     7141 cattgttgta cactttcgtg aaaactctat tgtcgtttaa atatatgttt ctccatatta
     7201 ccggcggatt aagccttcct gcttcagctt tgccggtata ggattcaaaa tatgcaagca
     7261 ttgtatttct gattttattt gtaagcttta aaatgctgtt tttgtatttt atataatttg
     7321 cattgaaatt gtctaaattg atttgccttt gttctttcaa tatcttgttg taatacagtg
     7381 cattggtgtc atcaccgaat tcatcatcga attctcctct ggtgaaatga aggtgtgtat
     7441 ttttgtggtt tccaacacaa aggccccgct ctattaaata ggttttctgc tgtgatataa
     7501 ttgaagcacc aaaatatttt cggataaact gtctttccga gtcatcctta ttttccagtc
     7561 ttactttgcg gacaggcttt ttggattttt ctttttcctg ttcatagtat acttctcttg
     7621 atgtttcagc ggaatccaaa tacaagtctt tcatggcttc catatctgaa tcgctgagct
     7681 tttcctcatc tgactgctgg acttgttttt gaactttttt cttgtttgtt tcggtattta
     7741 cctcataccg gccgtaattg aacctgaaat atgtttttat aatccagttc atttttgaaa
     7801 ttatttcttc tgtttctgca tcgccgaatt ctaggtcgtt taaaatatta agcaggttgc
     7861 ctgttatccc cgggggctct cccaatgctt tcctgaaatg ggcatttctt attacattca
     7921 ataaattatc atcaaaggtt tcagcttccc ctgccagtac tttccttgca tgactttttc
     7981 taaggctgtt aagtacaggt ctgtggtatt ttcctttatc aaatacggat ttttccaatc
     8041 ccagccagaa gagtttttca taaaactcgt gctcactgtc tgttttcagg taattaaaga
     8101 aacctctcag cagagggtag tcataatatt tatgaacagc tcctattata tagttccaat
     8161 agatatctgc ttccccgttt tcatcataag ccttgaactg agggtcgatg gaataatcgc
     8221 ctgacgcatt ccaaataata ttgagggctc ttttgttgtc ttgtgtattg ttcatggtca
     8281 cctgaaaatt tcattcctgc ttaaattatc ggctattctt aggcgaataa catcctctac
     8341 cagagtccgc tcaaagtcgt caaatgattt atttgcaatt cccatctgaa gtgctttagc
     8401 aggtgacaaa cccttgtcca tcagattaat tgaagcaaga agtcctctta agtcaactga
     8461 cttggttgaa atttcagaat tttcgctttt cagctgcaaa tcctggaaaa gtccggcaaa
     8521 ttgttcaata tattcatctc ttaaaccggg atattctctt tgcaataatt tctttaaatt
     8581 ctcttttgaa atgttaggca tgtttattac catgaagcgg gaggtcaagg cttcattcag
     8641 ctctcttgtg cctgcgtaac cgtaattcat ggttgctata aagcgggttt catcttctat
     8701 tgtaattttg tcataaccgg gtacatcgat tattcttctg tagtccaatg ttgcatgcag
     8761 gactgccacc gaatcatttt ttgccatgtt aatttcatcc aggaccccaa aaccgcccca
     8821 ttttgcacat tcatagatgg gaccgtttct gaatacaacc tggccgttct tgaatgtatc
     8881 tgttccaatc aatgatgtgg aatcagtgtt tacatgaaat gaaatatccc attttggtct
     8941 gccgaataca gctgacaagt tttctgccag aacatttttc cctgtagcct ttgagcccac
     9001 caaaagaagg ttctctccgc aaagaagagc tgtaattgcc tcttcccaga catcagaccc
     9061 gtaataattg tactttggcc tgggaatcct gtattccagc ttcgggtcaa ccttgtaaaa
     9121 ggttctgaac ttctcaatgt catttataag tctttctttt attccttctt ttnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaggttc tgaacttctc aatgtcattt
     9301 ataagtcttt cttttattcc ttcttttttc aagaaattta gcattgttaa cctccatgag
     9361 tgttttaccc gcagtctcat tcaccaatgt atttactccc tctggtcgta tacattggga
     9421 attcgttgcg aatgacctac ctgccatttt tctgaaaaaa gactcagaaa aatggggtta
     9481 gggcatcttt agaacagtat gtccattact tgtcttatat tatttactgc tattatttcc
     9541 atatttttta tttcttttga aatttcggca tttgcctcgg ctataaccgc tctttcaaat
     9601 cccattttgt tagcttccaa aagtcttttt tcgatagagt tgactcctct tatttctcca
     9661 gtgagcccta cctcgcctat caaaacggtt ttaaaatcaa cgggaatctc tttgaagctt
     9721 gatgccaaag cgcagagtac agccaaatcg caggcaggct cctttatctg cagtcctccc
     9781 gtaacattta taaatgtatc ggagctttgt atcgacaggc ctgctttctt ttctaatact
     9841 gctattagca tggataccct gtttttgtcg atgcctgttg ttaccctgct ggcaaatccc
     9901 accggtgaat atgtgactaa tgcctgtatt tctataagga cgggccttgt tccttccatg
     9961 gctgccgata ctacggtgcc gtaagcatca agaggcctgc cctttaagaa tacttccgaa
    10021 ggattttcaa cctctacaag ccccctgtcc ctcatttcaa aaattcctat ttcattggta
    10081 gagccgaagc ggtttttaac tgcccttaat attctgtagg aaaaatgtct ttccccctca
    10141 aagtataaaa cagtatcaac catgtgctcc aatactcttg gtcctgcaat tgctcctgac
    10201 tttgtaacat ggcctactat aaaggaggtc atgtttcttg tttttgtcat ccgcattacc
    10261 atggaagtta cttcccggac ctgggtgaca ctgcccggag cgcttgccac atccggagag
    10321 tatatagtct ggatggaatc aagcaccagc agatcaggct cttccttttc cacgtattca
    10381 attattatat caatattggt ttcagcaaga acataaaggt ccccgtctcc gatacccagc
    10441 ctgtcggccc ttattcttat ctgctcgccc gattcttccc cggatacata taaaaccttt
    10501 aaattttgtt ttgccgcatg gtctgcaacc tgcaaaagaa gggtggactt gcctatgccc
    10561 gggtctccgc ctacaagtat tagggagctt tttatgattc ctccccccag tactctgtcc
    10621 atttccatgc tgccggttga aattctttct tttttggcag gtgtaatatt gtttaatttt
    10681 tcaaccatac ccttgctgat gcctctctgg cttttagcat ctgttttttt gctttcaaat
    10741 tcctccgcaa aggtattcca ttcttcacag gatggacatt tgcccagcca ttttgccgtt
    10801 tcataaccgc attcctggca gacaaatttt gattttagct tagccatgat tcacctgctt
    10861 tcatgttttt attttaccta ctaactataa cgcaaaatgg cactttgtgt caatggaatt
    10921 atggcattac ccctgtcccc ttgtcatatg gacattgctc ccgtcaactt ttcatgtaac
    10981 gcatactaaa aatgctgcaa aattgtgaga caatttagga ccgtccccac tgtcccagtc
    11041 cccactgtcc caaaatatat ttttattgtt gacaagaggg ttgtcgaaat gtnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtccccac tgtcccaaaa tatattttta
    11221 ttgttgacaa gagggttgtc gaaatgtata ataaaaactg tttgaaagtt tagtaatttt
    11281 aaacttagag tttaataaaa ataggcggga agaaatgaat ataggagaaa atttaaagag
    11341 gataagagtt gccaattcac tcacccagca ggaagtagct gacaggtgcg aattgacaaa
    11401 gggttatatt tcacagcttg aaagagattt gacttctccg tccatagcaa ccttaacgga
    11461 tatactggaa tgtctgggta cggatttagc cggcttcttc aatgaagcag tggacaataa
    11521 aatagtattt aagcatgatg acgtgtttgt gaaagaaact gaagacaatt atgttataaa
    11581 ctggattgtt ccaaatgctc agaagtataa aatggagcct atactggtgg agctgggaga
    11641 agacggcaga acaaaagctg acgagcctca tgaaggtgaa gagttcggtt atgtgttaaa
    11701 cgggtctata ttgttaaatt acggcagtca ggtttataag gttaaaaagg gcgagtcctt
    11761 ttacattaag acaaataaaa ctcattatat agaaaatgca ggcaaaagca cggcgcgagt
    11821 actatggata agtactcccc catactttta aaggaggcag aagtggataa tgcaattata
    11881 aaattaaata atataactaa aaaatttgac ggagagactg ttcttgatga tattactttg
    11941 tctgttaaag aaaatgaatt cgtgacttta ttgggtccca gcggctgcgg aaaaactacc
    12001 acacttagaa taatcggtgg atttgaaact ccggatgagg gtgaagttta ttttgacggc
    12061 caactgataa atgatatccc tccctttaaa agacagctta acactgtttt tcaaaagtat
    12121 gctttgtttc ctcatttaga tgtttatgaa aatatagcat ttggccttag aataaaaaaa
    12181 atggatgaag ccgccataac cgccaaggtt gaaaaaatgc ttgagctttt caatcttaag
    12241 aattacggca agagaagaat tgaccagtta agcggtggcc agcagcagcg tattgccata
    12301 gcaagggctt tggttaatga gcccaaggta ctattgcttg atgagccatt aggggctttg
    12361 gatttaaagc tgagaaaaga aatgcagcat gagcttaaag ctatgcagaa gcagcttggc
    12421 attacattta tatatgtaac tcatgaccag gaggaagccc ttactatgtc cgatacaatt
    12481 gtagttatga acgagggtat tattcagcag atgggaaccc ccatagatat ttacaacgaa
    12541 ccggaaaatg ccttcgttgc ggactttata ggagagagca atatcctgga cggagttatg
    12601 attgatgatt acctggttga atttttagga aagagatttg aatgccttga tgccggtttt
    12661 ggaagaggcg aagaggttga tgtggttatc cgtcctgaag accttgattt aactcctgtg
    12721 ggacagggga tgctggaagg gattgtaagg tcaattacgt tcaagggtgt ccattatgaa
    12781 atggatattg cagccaatgg ttttacattt ttagttcatt caactgacat ggctcctgta
    12841 ggtgctacag tggggctgac cttgactcct gacgacattc atattatgga aaagggggaa
    12901 taggagtgaa agacagaaca aaatattttg catatcctta tatggtatgg ataattatat
    12961 ttattgctct gcccacgttt ttggtgcttt tatacagcat taccacaaag gaatcaaacg
    13021 ggcttacaac gatccagttt acatctgaaa atttcaggaa gttttttcag cccatttatt
    13081 tggacatttt gtgggattcc atttatttag ctgcagtatc aaccgcaata tgtttattgc
    13141 tgggatatcc tgctgcatat attatttcga atacccatat atcaaggaga aatacacttt
    13201 tgtttctttg tattctgccc atgtggatga atatgctcct tcgtacctat gcctggatga
    13261 ccatacttgg aaacaatgga atattaaata actttttcgc atttttaggt cttcccaggt
    13321 ttaatatgct gtatacaaga ggagctaccg ttatgggtat ggtatataat ttcattccct
    13381 ttatgatact tccaatctat actgcactga gcaaaatgga taagggtctt ttggaagcag
    13441 ctgatgatct gggagcagat aaaaagactg tttttaggaa ggttattctg cccttgagca
    13501 tgcccggtgt aatttcaggc gtcataatgg ttttcatgcc tgctgtcagc acatttataa
    13561 ttcctcagct tctgggagga aataagagca tgatgattgg aaacctggta gagaagctct
    13621 ttattttaaa cggagactgg aatttcgggt cagctatatc aattgtaatg atgctgataa
    13681 tacttttatc catgtcaatt atgaataaat ttgatgttga caaggaaggg ggagcaagac
    13741 tatggtaaaa aaatatttgt ctaaaatcta tatgggtata atatttatat tcctctatgc
    13801 tccaattata gttcttgccg tattttcctt caatgaatca aaatcaaggg gaaactggac
    13861 aggattttcg ttaaagtggt acattgagct tttccatgac catgatatac gcctggcctt
    13921 ttactacact atatcaatag caattattac ggcaatagta tcaactatta tgggtacaat
    13981 agcagcaata ggcataaatg ccatgaaggg cagacaaaag gccttaatac ttaatgttaa
    14041 ttaccttccg gttgttaatc ctgatattgt tacaggtata tccctgatga tcttatatat
    14101 ttcattcaat atggattttg gatttaatac aatgctgatt tctcatatag tattcagcat
    14161 cccctatgta atattgtcca tacttccaaa gctcaagcag cttgatgtac acatgaccga
    14221 agctgccatg gatttagggg caaccccaat gtacgctctc aggaaagtaa tacttcctga
    14281 aatcaaaccg ggaattataa cgggatttct gatggctttt acattatcca ttgatgattt
    14341 cattataagc tactttacaa agggcgaagg cgtaacaaat ctttcaattg ttatttattc
    14401 aatggcaaga agaggtgtcc gccccacaat caacgcgctg tcaacaataa tgctgactgt
    14461 agtactcttg ctgatgatat tgataaacaa aagaaacaac ggccaggata agtcgaaaaa
    14521 aataaaccaa agtgaggtat ataaatgaaa agtaaactga ttttatttgt ttcaatttta
    14581 tgcttgctat taattgctgc aaccggatgc agcactgaag aaaaacaaac attgaaagtt
    14641 ttaaactatg acatttacat tgataaaagc ttattatctg aattcgaaaa ggccaacaat
    14701 gtaacaatca aatatgacac atattcaact cctgaagata tgtatatcaa ggtgaaatcc
    14761 ggtgcctcaa aatatgattt gattatttca actgaatata tgattgaacg catgattagt
    14821 gaaaacctgg ttaataaatt gaattttgat aatatcccca attataaata tatcagcgaa
    14881 gattttaaaa accatgtgta tgacccgaat aacgaatact cggttcccta tttctggggg
    14941 actttaggaa tattgtacaa caaaaccact gttgatgcat catcagacag ctgggctatg
    15001 ctctgggata aagctcatgc tcaaagaata ataatgatgg attcgcaaag ggattcattt
    15061 gcagccgcac ttaaattatt gggatattca atgaacacag ttgatgaaag agagcttgat
    15121 gaagcgaaag ccctgttgat tgagcaaaaa ccccttgtaa tggcttatat tacagacggg
    15181 tcgcctgcta taatggtaaa tgaagaagct gatatggccc ttgtctggtc gggggaagct
    15241 gttgcagcga tgtctgaaaa tgaaaatttg gattttgtaa ttccaaagga aggcagcaat
    15301 atatggattg atgcaatggt tattccatca actacggaaa accaggaatt agctgaaaaa
    15361 ttcataaact tcctgtgcac tacagaagca acattgagaa atatagatga agtatggtac
    15421 tcgacagttc atacggaggc tttggagcag gtagatgaag agctgttaaa caacccggct
    15481 ttcaacattc ctcgtgagga tatagtaaat atggaaatgc tgagggaccc gaaagaattt
    15541 attaatctct actctgaaag atggactgaa ttgatggccg aataaacagc atagaacaaa
    15601 agcggggcaa tcttcaagtg a
//
LOCUS       DAOPUT010000033        15596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3845_length_15596_cov_20.165112, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000033 DAOPUT010000000
VERSION     DAOPUT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15596
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3845_length_15596_cov_20.165112"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..2231
                     /locus_tag="PKK75_04435"
     CDS             273..2231
                     /locus_tag="PKK75_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNZ82875.1"
                     /translation="MAIEKMASLKVIGKLEEIEPIIKELILYENVSINAGSDHYGNSY
                     LIHEYEAEIEESSEYNIQDYFGIESECKDAILLVEDISKELGIKLNLEKDLIEKNDIS
                     YEQAIEMLNNYKSTNKKITDEIKNKKEEKEKLLYYKKVMDSIFDNSIELKKIADLNFF
                     DYEIGILSNESRVKLKNNYESFIGIINRIGVVKPTAENLYLIIYLSQFKEQTTNFLKS
                     LNWRRFNLPEKYTGTPAQISEQITEEIDVLSKGIKELSYLSETKNKNVNSSMNRIYNA
                     LKLEERTIEIAKEADFGDNIFLLNVWIRNSDINNFRESITKAYDKVKIIEAEKNGSSP
                     PTVLKNNWFFRPFELIVKLYGLPSYRELDPTPFMALTYCLAFGIMFGDIGQGFIYFMA
                     GYLLSKKMDLAGQLLMRLGATSMVFGLVYGSLFGLEKHQLPWLPSLFEGGPLSAENIP
                     KILAVGIAYGIIALSGSFIYNIINSLKIGDKEEGLFGKNGVFGYLFFLSFIFTTLSLV
                     GLVKIPLYVPLLLLVAFLAVIVLKEPLANALDKKELFPHGAGAYFTENIFESIETMLS
                     TLSNTVSFVRVGAFALNHAGLFLAFMIISELVDNIVLKILILALGNILILVLEGLIVF
                     IQGLRLQFYEMFNKYFVGEGIEFKSVKL"
     gene            2265..2708
                     /locus_tag="PKK75_04440"
     CDS             2265..2708
                     /locus_tag="PKK75_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HNZ82876.1"
                     /translation="MQILLIILAAAVSAGTIIYGVKAMKNGKKGNVRKAVITTISAFG
                     LIMLGAVISTIFGGNAFAETAAQTAETAKASMGLGEGLKYLSAALCTAIATLATAKAV
                     SSVGSSAVGAVSEDPSLLGKTLIFVGMAEGIAIYGMIISILILFA"
     gene            2736..3044
                     /locus_tag="PKK75_04445"
     CDS             2736..3044
                     /locus_tag="PKK75_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNZ82877.1"
                     /translation="MKSFLISDNRDTYVGLQLAGINGKIVHNREDALKEMRKVIQSDD
                     IGILILTEKIVDMISKEVMEYKVKSKTPLIIEIPDRLGSIRKSNLVEEYIRNSVGIRV
                     "
     gene            3053..3679
                     /locus_tag="PKK75_04450"
     CDS             3053..3679
                     /locus_tag="PKK75_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HNZ82878.1"
                     /translation="MVTIEQKLELFRKLLNQSMYLKVEEELSKLEKEFQMKVQQTEET
                     IDIAAQEIVDRAKKKAELRHAENTGKSQITMKKEIMSIKEKYLEVFMNNFKGRLEEFI
                     ESKEYKTFLYKNILKLNNELNDCDKLDLNIYLSTKDFNEYRDFIKTEIAKNHGVSFIT
                     FDIMGGFIAEVPSKGIKYDLSIDTILEDNKAYIMQTLFEALKAGERND"
     gene            3672..5441
                     /locus_tag="PKK75_04455"
     CDS             3672..5441
                     /locus_tag="PKK75_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNZ82879.1"
                     /translation="MIDEGIITGINGPVVKGLNMRSFKVNEMVTVGNKKLIGEVVSVE
                     GDVATVQVYEETEGLKLSEKIYSTGSPLSVTLGPGIIGNIFDGIQRPLNIIREISRDE
                     FIPEGIGLLSIDDKKLWDVNIIVKKNDILKAGDVYATVQETSNILHKLLVPFNIKGTV
                     KEVKNNGKYSINDTIAVLEKENKETVNLTLCSKWPIRKPRPVTKRLPLYKPLITGQRV
                     IDSFNPIAKGGTVGLPGGFGTGKTVTQHQIAKWCDADIIVYIGCGERGNEMTNVLEEF
                     PALIDPRTGRPLMERTILIANTSNMPVAAREASIYTGITLAEYYRDMGYDVAIMADST
                     SRWAEALREISGRLEEMPAEEGYPAYLASRLAQFYERAGCVRTLGGDEATVTIIGAVS
                     PPGGDFSEPVTQNTKRFVSTFLALDKKFANARHFPAINYLTSYSGYIDILSDWFEENV
                     AIDMLDLRHKMVVLLQEEEKLNEIVQLVGEDVLPNDQALVLETARLIKKSYLQQNALH
                     PEDTFVTLEKQYKMLKVIDAFHESAMKCVKMGIPTSEIRKEQVLQEVYKMKFDVPNNN
                     IELLDDLKDRVEVSYSILRQKYE"
     gene            5451..6830
                     /locus_tag="PKK75_04460"
     CDS             5451..6830
                     /locus_tag="PKK75_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNZ82880.1"
                     /translation="MRKEYLKLHEINGPLIVLTGIDDAAYGETVSIKVDNKEKRLGKV
                     IKLEGDKVIVQVYEGTSGISTDNASVKFLGEPLAIPLSKEILGRTFNGVGNPIDGGLQ
                     IVSSNKININGRPINPVSRKYPKNFIQTGISAIDALMTIIRGQKLPIFSGNGLDHNQL
                     AAQIVKQAKIKGATKDNFAVIFGAMGVRHDDADYFRKSFESAGVLDHVVMYINKADDP
                     IIERLSTPRCALTAAEYLAFEQNMHVLVVLTDMTSYAEALREVSSAKEEVPSRKGYPG
                     YLYSDLSTLYERAGMLEGKEGTITLMPILTMPNDDITHPVPDLTGYITEGQIVLSRDL
                     NQKGLYPPIDILPSLSRLMKDGIGKEYTREDHADLQSQIFSSYAKVQDIRGLAQIIGE
                     DDLSDTDKLFLKFGHELENKFIAQGNENRNIIETLDLGWNILSILPVSELDRLKAETI
                     EKYYIYDRK"
     gene            6836..7444
                     /locus_tag="PKK75_04465"
     CDS             6836..7444
                     /locus_tag="PKK75_04465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNZ82881.1"
                     /translation="MAVQIAPTKTNLIKAKSSLDLSKRGYELLNKKRTVLIKEMFAYV
                     EKARTMNKEIYEVIAEGYAALQKANITLGVKNVEDMSLGVPNDEDFDILLRSIMGVDI
                     PTVKYKEIDVQPTTNFYNTNAAFDEARKKFKEVKFKIYDLTGLENSIFKLAKEIEKTQ
                     KRTNALEFIIIPRLIEEVKFIQDVLEEKEREDFFRLKRLKGK"
     gene            complement(7482..7742)
                     /locus_tag="PKK75_04470"
     CDS             complement(7482..7742)
                     /locus_tag="PKK75_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNZ82882.1"
                     /translation="MKKVKVFYMEGCPHCRRAFKILDELKANNPEYAKIHMEYIDENK
                     DVKAANAHDYYYVPTFYMDEVKLHEGVPTVEKIERVLKEAIK"
     gene            complement(7744..9213)
                     /gene="hemZ"
                     /locus_tag="PKK75_04475"
     CDS             complement(7744..9213)
                     /gene="hemZ"
                     /locus_tag="PKK75_04475"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HNZ82883.1"
                     /translation="MFKFYFSGHDYEYEAGNALRVFDLNTDYEIMYSSFEGRGLSLVS
                     KLDEGEIISAKAQLYDDNELLYQSYFKADDIILEKYGLKKLKKTLVIKTIHNVLKSYY
                     KVEPDYGILTGVRVVKILLTAKRQNKSDEEINDILRNTYEIKEEKIKLLWNILKIEEK
                     YIDENINNKNYNLYIGIPFCPSKCSYCSFTSFVNCPDKKINSYLDTLIYEIEKTIEHA
                     KKNELSLNTIYIGGGTPSILNENQIDKIFDVIKNYYDLSKIKEITFEAGRPDTINKEK
                     LICLKSNYVNRISINPQTMNDNTLLSVGRKHLSCDIVEKYLLAREIGFQTINMDVILG
                     LPGEDEADVKNTIEILAGLNPENITVHSLAYKKHSELTRESVKLQKDYDLIKKMHDII
                     QEVCAKKRYKPYYMYRQKNIKGNSENIGYTRDGRECIYNMVMVEELETILGCGLGASS
                     KIITGENKHETIRNFKSLEEYSNRIDEIINKKKSIRRNI"
     gene            complement(9288..9914)
                     /locus_tag="PKK75_04480"
     CDS             complement(9288..9914)
                     /locus_tag="PKK75_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ82884.1"
                     /translation="MLFEALEVGIYGANCYIAGSEETMEAAIIDPGADFNKIDSKLKE
                     LGLTPKIIILTHYHGDHIGAVKELTDKYGLKVYIHKDDAEALGDGSMNLTKSMLGKGI
                     SIKADVELSDGDEISLGELNFKIIHTPGHTRGGISIKVGNIMMTGDTLFRSSIGRTDF
                     PGGSFEEIINSIKDKIFTYDDDTLIYPGHMSPTSIKAEKKFNPFVKIS"
     gene            complement(9924..10373)
                     /gene="dtd"
                     /locus_tag="PKK75_04485"
     CDS             complement(9924..10373)
                     /gene="dtd"
                     /locus_tag="PKK75_04485"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNZ82885.1"
                     /translation="MRAVVQRVESANVKVEGKITGEIDHGILLLLGVEERDEAADLEY
                     MCEKIPNLRIFEDENGKMNKSLLDTGGSILVISQFTLLGDARKGRRPSFTDAARPEKA
                     IPMYEEFIARMKEKNITTESGVFGAEMKVQLINDGPVTILLDSKKVF"
     gene            complement(10418..>12036)
                     /locus_tag="PKK75_04490"
     CDS             complement(10418..>12036)
                     /locus_tag="PKK75_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNZ82886.1"
                     /translation="YIDPEGYEDLLKKVQEKSEKRKGYISHIIEVIHDKLKEQGMEAD
                     IKGRPKSLYSIYKKMYFKNRSFEEIYDMIAIRIIVDNIKDCYGALGTVHTLWKPVPGR
                     FKDYVAMPKPNMYQSLHTTVIGPEGEPFEIQIRTLEMHQTAEYGIAAHWKYKEGIDET
                     TKYEEKLNWLRQMLEWQQETNDPQEFMEALKIDLYTDEVFVFTPKGEVVNLPTGSTPI
                     DFAYRVHSAVGNKCVGAKVNGRMVPIDTKLKTGDQVEVLTSQASAGPSRDWLKIVASS
                     QAKSKIRKFFKEKDKDFNEDKGKELVEREIKKQGFQVGDLLKDDWIKTVADKYNMHTA
                     SNLYAAVGNGSITENQIFNRLKQIYLEKNKDKLEIEKIENLEKTAEKTPQRVEKHASG
                     VIIKGIDNIKIRFSKCCNPVPGDAIVGYITRGRGVSVHRADCTNIHDLSDSEDQRFIE
                     VQWDYKEKATFISELQIKALDRPRLLQDITSMYVDAKLNAISLNLRINKEKIAIADIG
                     FEINETKQIEDLIKKLKRLDGVLEVYRTKK"
     assembly_gap    12037..12136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12137..12423)
                     /locus_tag="PKK75_04495"
     CDS             complement(<12137..12423)
                     /locus_tag="PKK75_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ82887.1"
                     /translation="MLGNIINQIESYNPNADLEIVVKAYNYAEMAHSNAQTRFSGERY
                     FVHPYNVALILADLHMDVQTICAGLLHDVVEDTGISFDEIKKEFGEEIANMV"
     gene            complement(12537..13055)
                     /locus_tag="PKK75_04500"
     CDS             complement(12537..13055)
                     /locus_tag="PKK75_04500"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNZ82888.1"
                     /translation="MDLKEKIRVIEGFPAEGISFKDITTLLKDSEALYECINEMAERF
                     KDVQVDIVAGPESRGFIFATPLAYLLRTGFVPVRKPGKLPAETIKHEYDLEYGTDSLE
                     IHKDAIKKGQKVLIIDDLLATGGTMFATAKLVEKLGGEVVGLGFLIELKDLKGREKLK
                     EYKVESLVTYDK"
     gene            complement(13072..14985)
                     /gene="recJ"
                     /locus_tag="PKK75_04505"
     CDS             complement(13072..14985)
                     /gene="recJ"
                     /locus_tag="PKK75_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNZ82889.1"
                     /translation="MKTVKLKVNEYSNDEIERISRRFNLSKTSAKILLNRNISSFDDI
                     EQFLDPDFKYFESAKNYKDLDKGCKRVIEAINNNERILIYGDYDVDGVTSISQFVVLL
                     KKTGAQVDYYVPERESEGYGISTDFVDRLKSGEISIDLLITVDCGIAENEKIDEITKL
                     DTDVIIIDHHQCREELPHAYAIINPKQKDCPSENKHLCAAGLSFKFLTHLNEYLKIND
                     IEDVLLEYACLGTIADIVDLVKDNRIIASRGLSRINNTRIAGIKKLIQVSGIKDKEIK
                     SYHIGFMLAPRINASGRMDTAKKAIRLMLTEDEEEAQILALELESLNNARKEAESEIF
                     NEAVKKIESDFLYKNNVIVIHGYDWHEGVLGIVASKLTEKYYKPCVVISVKDGTGKGS
                     ARSLEHVDIFESLNACDSYLEKYGGHKLAAGLTVLEENINSFTNELNNYIGSNDDEEY
                     YLRADAILGINDMNLRLFDEINNFEPFGSGNQKPILALRDVSLKNIRKVGPEGKHISF
                     MLSNEGQSIPVIGFGKITLLGKVLSMPKSFIVSLSENLYNGSRSIQLILQDVEDREEL
                     DYKIDAGKLKTLEFLINKSKSKIIRTDIFMLVEKLNKRYNTKITAEEIICMLKKAENI
                     QYALKNEILYIKK"
     gene            complement(14957..15496)
                     /locus_tag="PKK75_04510"
     CDS             complement(14957..15496)
                     /locus_tag="PKK75_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HNZ82890.1"
                     /translation="MQIGFIVILIIAIFVAIFAIQNGTPVPMDLFFARYEMPLAVIMM
                     ICLILGAVMVLILGTTRQFKKRSEYKELKNRIKTLEGEKAQIETNIKTLEKEKADFTA
                     KVDALEKERQALQDENTGFIEKVTKLEDKIKFQEEELTGKNQEIEMLKIQNIDKFRVE
                     IADEGTIQIDNENSETESQ"
BASE COUNT         4816 a         2776 c         2671 g         5233 t
ORIGIN      
        1 tggcattttc caataaggaa agaaattgag gtttgtcatt ttcaaatttt tcttgacaat
       61 cggaaaaaat ttctgccaaa gaaagctgtt tatgtggtat catatttacg taactccttt
      121 cgaggttttg gtggatttgt ttattgacaa ttcaatttta ccataaatca gtgaggagtt
      181 acagcttttt tatacaagaa aaccctgtaa tttcaggggt tccggcgttt cacaaacgcc
      241 tattggtaaa tataatatga aaggagatat gtatggctat agaaaaaatg gcttcgttaa
      301 aggttattgg taaattggaa gaaattgaac caataatcaa ggaactgata ctgtatgaaa
      361 atgtaagtat aaatgcaggg tcagaccatt acggcaatag ttatttaata catgaatatg
      421 aagctgaaat agaggaatct tctgaatata acatacaaga ttattttgga atagaatcag
      481 aatgcaaaga tgcgattttg ttggtagaag acatatctaa agaattaggt atcaaattaa
      541 atttagaaaa agatttaata gagaaaaatg atatcagcta tgaacaagct attgaaatgt
      601 taaataatta taagtccacg aataaaaaaa taacggatga aattaaaaat aaaaaagaag
      661 aaaaagagaa attgctatat tacaaaaaag taatggacag tatttttgat aattccatag
      721 aacttaaaaa aatagcagat ttgaatttct ttgattatga aataggtata ttatcaaatg
      781 aaagcagagt aaagttaaag aacaactacg aaagctttat tggtattata aataggatag
      841 gtgtagtaaa gccaacagct gaaaacttgt atttaattat atatctcagt cagtttaaag
      901 aacaaacaac caacttttta aagtccttaa attggagaag gtttaatttg ccggaaaaat
      961 atacaggaac accggctcaa atatcagaac aaatcactga agagatagac gtattaagca
     1021 agggaatcaa agagttatct tatttatctg agacaaaaaa taaaaatgtt aattcttcga
     1081 tgaatagaat ttacaatgca ttaaaattgg aagagagaac aattgaaatt gcaaaggaag
     1141 cagatttcgg cgataatatt ttcctattga atgtttggat aagaaattct gatataaata
     1201 attttaggga aagtatcact aaggcatatg ataaagtaaa aattattgaa gcagaaaaaa
     1261 atggttcctc cccccctaca gtattaaaaa acaattggtt cttcaggcct tttgaattaa
     1321 ttgtaaaatt gtatggactg ccttcataca gagagcttga ccctacaccc tttatggcct
     1381 tgacatactg tttggccttt ggaataatgt tcggtgatat cggtcagggg tttatatact
     1441 ttatggcagg atatctttta agcaaaaaaa tggacttggc aggacaactg ttaatgcgcc
     1501 ttggagcaac atctatggta ttcggtttag tctacggcag tttgttcgga cttgaaaaac
     1561 atcagttgcc atggctccca agcttatttg aaggcggacc cttaagcgcc gaaaatatac
     1621 ccaagatatt agctgtaggt attgcatacg gaattatagc cttatccggt tcctttattt
     1681 acaacattat aaattcgctg aaaatcggcg ataaggaaga aggtttgttt ggtaagaatg
     1741 gtgtatttgg atacttattt ttcttaagtt ttatattcac tactctttcc ttagtcggtc
     1801 tggtaaagat acccctatat gttccgctgt tgttattagt tgcatttctg gcggtcattg
     1861 tattaaagga accattggcc aatgccctgg ataaaaaaga attgtttccc catggtgcag
     1921 gagcctattt tacggaaaat atttttgaat caattgaaac tatgttaagc accttaagca
     1981 atacagtatc ttttgtgcgt gtaggtgcgt ttgcattaaa ccatgccggc ttgtttttag
     2041 cctttatgat tatatccgaa ttagtggata atatagtctt gaaaatttta atattggcat
     2101 taggcaatat attgatattg gtattagaag gtttaatagt atttattcaa ggtcttcgtc
     2161 tgcaatttta tgaaatgttc aataaatatt ttgtaggtga aggtatagaa tttaaatcag
     2221 taaaattata ataaatcaaa aacatttaga agaagggaaa aactatgcaa atcttattaa
     2281 taattttagc agctgcagta tcagctggaa caatcattta tggagtaaaa gcaatgaaaa
     2341 acgggaaaaa aggcaatgta agaaaggcag taataactac tatatcagct tttggcttaa
     2401 ttatgcttgg agctgtaata tctacaatat tcggtggaaa tgcatttgct gaaacagcag
     2461 cgcaaacagc tgaaacagca aaagcttcta tgggattagg agaaggcttg aaatatttat
     2521 ctgctgcatt atgtacagca attgcaacat tagcaacagc caaggcagtt tcttcagttg
     2581 gttcatcagc tgtaggagct gtttctgaag atccttcatt acttggtaaa acattaatat
     2641 ttgtaggtat ggctgaaggt atagctattt atggcatgat tatttctatc ttgattttat
     2701 ttgcttaaca aaaatttaat gaaaagagta aaaatatgaa gtcattttta ataagtgata
     2761 atagagacac atatgtgggt ctgcagttag cgggtattaa cggtaaaata gtgcacaata
     2821 gagaagatgc ccttaaagaa atgagaaaag tcattcaaag tgatgatata ggtatcttaa
     2881 tattgacaga aaaaatagtt gatatgataa gtaaggaagt tatggaatat aaggttaaat
     2941 ctaaaactcc tcttatcatt gaaattcccg atagacttgg ctctataaga aaatctaatc
     3001 ttgtcgaaga atatatcaga aattcagtag gcattagagt ttgaggtgat ttatggttac
     3061 aatagaacaa aagttggaac tctttagaaa gctgttaaat caatcaatgt atttaaaagt
     3121 tgaagaagaa ctgagtaagc tggagaagga atttcagatg aaagtccaac aaactgaaga
     3181 aacaatagat atagcagccc aggaaattgt agacagagcg aagaaaaagg ctgaattgag
     3241 gcatgcagaa aatacaggca aatcccaaat aacaatgaaa aaagaaatca tgtccattaa
     3301 agaaaaatat cttgaagtat ttatgaataa ctttaaaggc agattagaag agtttattga
     3361 gtcaaaagaa tataaaacct ttttatacaa aaatatatta aaattaaata atgaacttaa
     3421 tgactgtgat aaattggatt taaacattta tttgtcaaca aaagatttca atgaataccg
     3481 tgactttata aaaactgaaa tagcaaaaaa tcatggggtc tcttttataa cctttgacat
     3541 aatgggtgga ttcatagcag aagtaccatc taagggtatt aaatatgatt tatcaattga
     3601 tacaatattg gaagataata aagcttatat aatgcagact ttgtttgaag ccctaaaggc
     3661 aggtgagcgc aatgattgat gaaggtatta ttacgggaat aaacggtcct gttgtaaaag
     3721 gcttaaatat gagatccttt aaagtaaatg aaatggttac tgtgggaaat aaaaaactta
     3781 taggtgaagt tgtatccgta gagggcgacg ttgctacagt acaggtatat gaagaaacag
     3841 aaggattaaa attaagcgaa aaaatatatt ctaccggctc ccctttatca gtaaccttag
     3901 gacccggtat tataggcaat atttttgacg ggatacaaag accactaaat ataattcgag
     3961 agatttccag ggatgaattt atcccggaag gaataggact tttgtcaata gatgacaaaa
     4021 aattatggga tgttaatata atagttaaaa aaaatgatat attaaaagcc ggtgatgttt
     4081 acgctactgt acaagaaaca tcaaacatcc ttcataaact gctggttccc ttcaatatta
     4141 aaggaacggt taaggaagta aaaaacaacg gcaaatacag cattaatgat acaatagcgg
     4201 tgcttgaaaa agagaataaa gaaactgtta acttaacatt atgcagcaaa tggcctatta
     4261 gaaaaccaag gcctgttaca aaaagacttc ctttatataa acccttaatt acaggtcaaa
     4321 gagttattga cagttttaat cctattgcca aaggcggtac cgtaggtctt ccaggtggat
     4381 ttggaaccgg caaaactgtt acccagcacc agattgcaaa atggtgcgat gcagatatta
     4441 ttgtttacat cggctgcggc gaacggggta atgagatgac aaatgttttg gaagagttcc
     4501 ctgcactaat tgaccctcgc accggaaggc ctttgatgga gagaacaata ctaattgcaa
     4561 atacatcaaa tatgcctgtt gcagcccgtg aagcaagcat ttacaccgga attacattag
     4621 ctgaatatta cagagacatg ggttatgatg ttgccattat ggcagactcc acatcccgat
     4681 gggcagaagc actacgtgaa atttcaggac gtttggaaga aatgcctgct gaggaaggtt
     4741 atcctgctta tttagcatca agattggcac aattttacga aagagcagga tgcgtaagaa
     4801 ctttaggcgg agatgaagcc acagttacaa taataggagc agtatcacct ccgggcggag
     4861 atttttcaga acctgtaact cagaacacta aaagatttgt ttcaaccttt ttagctttag
     4921 ataaaaaatt tgcaaatgca agacattttc cggcaatcaa ctatttaaca agctattcag
     4981 gctatataga tattttgagt gattggtttg aagaaaatgt ggcaatagac atgctggatc
     5041 taagacacaa gatggtggtt ttattgcagg aagaagaaaa actaaatgaa attgttcagc
     5101 ttgtaggtga agatgtattg ccaaatgatc aagcattagt tctcgaaact gcaaggttaa
     5161 tcaaaaaatc ctacctccag caaaatgcac tccaccctga agataccttt gttactttgg
     5221 aaaaacaata taaaatgtta aaagtaatag atgccttcca tgaaagtgca atgaaatgcg
     5281 tgaaaatggg aattcctaca tcagagataa gaaaggaaca agttttgcag gaagtataca
     5341 aaatgaagtt cgatgttcca aataataata ttgaactttt agatgattta aaagataggg
     5401 ttgaggtatc atacagcatc ttaagacaaa aatatgaata gggagaaaaa atgagaaaag
     5461 aatatttaaa attacatgaa attaacggac ccctaatagt tcttaccgga attgatgatg
     5521 ctgcctatgg tgaaacagtt tcaataaagg tagataataa ggaaaaaaga cttggcaaag
     5581 taattaaact tgaaggagat aaggttatag tacaagtata tgaaggtaca tcaggaattt
     5641 ctactgataa tgcatccgta aaatttctgg gtgaacctct tgctattcct ttatcaaaag
     5701 aaattttggg aagaactttc aacggagtag gtaatcctat tgatggtggt cttcaaattg
     5761 tttcctctaa caaaattaac ataaacggaa gaccgattaa tcccgtatca cgtaaatatc
     5821 cgaaaaattt tatacaaaca ggaatttctg caatagatgc attaatgacc ataataaggg
     5881 gacaaaagct gcctattttc tcaggcaatg gtttagacca caatcaactt gcggcacaaa
     5941 tagtaaaaca ggctaaaatt aaaggagcaa caaaggataa ctttgctgtt atatttggtg
     6001 caatgggtgt aagacatgat gatgccgact acttcagaaa aagttttgaa tcagcaggag
     6061 ttttagacca tgtggtaatg tatatcaaca aagccgatga tcctattatt gagagattat
     6121 ctactcccag atgtgctctt actgcagcag aatatttagc atttgagcaa aacatgcatg
     6181 ttcttgtggt tctaacggat atgaccagct atgcggaagc cttaagagaa gtttcttccg
     6241 ccaaggaaga agttccgtcc cgtaagggtt acccgggata tttgtacagc gacttgtcta
     6301 cattatatga aagggcggga atgcttgagg gaaaagaagg aactataacc cttatgccta
     6361 ttttaacaat gccaaacgat gatattactc atcctgttcc tgatttaacc ggatatataa
     6421 ctgaagggca aatagttcta agcagagatt taaatcaaaa aggattatat ccgccaattg
     6481 atattcttcc atccttatct cgtttaatga aggacggtat aggtaaagaa tacaccagag
     6541 aagatcatgc agatttacaa agccagatat tttcatcata tgccaaggta caggatatta
     6601 gaggattagc tcaaattatt ggtgaagatg atttaagcga tacagataaa ttatttttaa
     6661 aattcggaca cgagttggaa aataagttca tagctcaggg taatgaaaac agaaatatta
     6721 ttgagacctt ggatttagga tggaacattt tgtccatact tccggtatcc gagttagaca
     6781 ggctaaaggc tgaaacgatt gaaaaatatt atatttatga caggaagtga tggttatggc
     6841 tgtacagatt gcccctacta agacaaacct tattaaagct aaatcatcat tagacctgtc
     6901 aaagagaggt tatgagctct taaataaaaa aagaacggtt ttaattaaag aaatgtttgc
     6961 ttatgtggaa aaggcacgaa ctatgaacaa ggaaatctat gaagttattg ccgaaggata
     7021 tgcagccctg caaaaagcaa atataacatt aggtgttaaa aatgtggaag atatgtcttt
     7081 aggcgtacca aatgatgaag attttgacat tttgttaaga agtattatgg gagtagacat
     7141 acccaccgta aaatacaaag aaatcgatgt tcagccaaca accaactttt ataacactaa
     7201 tgccgccttt gatgaagcaa gaaaaaaatt caaagaagtt aaatttaaaa tctatgatct
     7261 tacaggattg gaaaactcaa tctttaagtt agcaaaagaa atcgagaaaa ctcaaaaaag
     7321 aacaaatgca ttagaattta taataatacc ccgattaatt gaagaagtca aattcattca
     7381 agatgtattg gaagaaaaag aaagagaaga tttcttcaga ttaaaaagat taaaaggtaa
     7441 ataagaaacg agctgaacca gtgttagctc gtttttttta tttattttat cgcttctttc
     7501 aatactcttt caattttctc caccgtagga actccttcat ggagcttcac ctcatccatg
     7561 taaaatgtgg ggacatagta gtaatcatga gcatttgcag ctttaacgtc cttgttttca
     7621 tctatgtatt ccatatgtat ctttgcatat tcagggttgt tagcttttaa ctcgtcaagt
     7681 atcttaaatg ctctcctgca atggggacaa ccctccatat aaaaaacttt tacttttttc
     7741 attttatatg tttctcctga tactcttctt tttgtttatt atttcatcaa ttctatttga
     7801 atattcttct aagcttttaa aatttcgtat cgtctcatgc ttattctctc ccgtgattat
     7861 tttgcttgaa gcaccgagac cgcaccctaa gatggtttcc agttcttcta ccataaccat
     7921 gttgtagatg cattccctgc catccctggt atagccgata ttttctgagt tccccttaat
     7981 attcttctgt ctatacatgt aatagggttt atatcttttt tttgcacaaa cctcctgtat
     8041 tatatcatgc atctttttaa taagatcata atctttttgt aatttgacac tttccctggt
     8101 aagctcagag tgttttttat aagccaatga atggactgta atattttcag gatttaaccc
     8161 tgcaagaatt tcaattgtat tttttacatc tgcttcatct tcccccggca atcccaaaat
     8221 aacatccatg tttatagttt gaaatccgat ttctcttgcc agtaaatatt tttcaacaat
     8281 gtcacaggat aagtgctttc tccctaccga tagtaaagta ttatcattca tggtctgggg
     8341 atttatggat attctgttta cataattact ttttagacag ataagttttt ctttgtttat
     8401 ggtgtcaggt cgtccggctt caaatgtaat ctctttaatt tttgacaggt cgtaataatt
     8461 ttttatgaca tcaaaaattt tatctatctg gttctcattt aatatggagg gtgtaccacc
     8521 ccctatgtag attgtattca gggacagttc gttctttttt gcatgttcaa ttgttttttc
     8581 aatttcatat attaaagtat ccaggtagga atttattttt ttgtcaggac aatttacaaa
     8641 tgaggtaaag gagcaatagc tgcacttgga agggcagaaa ggaattccta tgtataggtt
     8701 gtagtttttg ttgtttatat tttcgtcaat atacttttcc tctattttca aaatattcca
     8761 tagaagcttt attttttctt cttttatttc ataggtattt ctcagtatat catttatttc
     8821 ttcgtcgctt ttattttgcc gttttgcagt gagtaaaatt ttaactactc ttactcccgt
     8881 taaaattcca tagtccggtt ccaccttgta atatgatttc aaaacattgt gtattgtttt
     8941 tatcactaat gtttttttca gctttttaag accgtatttc tctaaaatga tatcatctgc
     9001 cttgaaatat gattggtata atagttcatt atcatcgtaa agctgcgctt ttgcactgat
     9061 aatttccccc tcatcaagtt ttgacacaag agataagccc cttccttcaa aggagctgta
     9121 cattatttca taatccgtat ttaaatcgaa taccctcagt gcatttcctg cctcgtattc
     9181 atagtcgtga cctgaaaaat aaaatttgaa cattatttat acctattaaa attttttgtc
     9241 atcatgtgca atagcgaagg gcgtttccca taccggtaaa aaagtggtca ggatatcttc
     9301 acaaatggat taaatttttt ctccgccttg attgatgtgg gtgacatatg acctggatag
     9361 attaaagtat cgtcatcata tgtaaaaatc ttatccttta tagaatttat tatttcctcg
     9421 aaggaccctc ccggaaaatc agttctgcct atggaagatc taaacaaagt atctccggtc
     9481 atcataatat ttccgacctt tatacttatg cctccccttg tgtgtccagg tgtatggatt
     9541 attttaaaat ttaattcacc taagcttatt tcatctccat cgctgagctc tacatccgcc
     9601 tttattgaaa tacctttccc tagcattgat tttgttagat tcatgcttcc gtcgccgagt
     9661 gcttctgcat catccttgtg aatataaact ttcagcccgt atttatccgt taattctttt
     9721 accgcgccta tatggtcccc atgatagtgg gtaagtataa ttattttcgg ggttagcccc
     9781 agttctttta atttgctgtc aattttatta aagtcagctc cagggtctat aattgcagcc
     9841 tccatggttt cttcacttcc tgcaatataa cagttggcac cgtaaatgcc aacttccaat
     9901 gcctcaaata acataatttc ctcctaaaaa acctttttgc tgtcaagtaa tatggttaca
     9961 gggccgtcat ttattaactg aaccttcatt tctgcaccaa atacccctga ctctgttgta
    10021 atattttttt ctttcatcct ggctataaat tcttcataca tgggtattgc cttttccggc
    10081 cgggctgcat cagtaaagct tggccttctt ccttttcttg catctccaag cagggtaaac
    10141 tgtgaaataa ccaaaatgct tcctcctgta tcaagaaggc ttttgttcat tttaccgttt
    10201 tcatcctcaa atattctaag atttggaatt ttctcacaca tatattccaa atctgctgct
    10261 tcgtctcttt cttcaacacc cagcaaaagc aaaattccat ggtctatttc tcctgttatt
    10321 ttaccttcaa cctttacatt tgcactttct actcgctgta caactgctct catttataaa
    10381 gtacggggac attcctttaa ttgttccccc ctccttttta ctttttggtt ctgtaaacct
    10441 ccagtacacc gtctaatctt ttaagcttct taatcagatc ttcaatttgt tttgtttcat
    10501 ttatttcaaa tcctatatcc gcaattgcaa tcttttcctt atttattctc aaattaaggg
    10561 atatggcatt aagctttgca tctacataca tggatgttat atcctgcaat aatcttggtc
    10621 tgtccagtgc cttgatctgc agctcgctta taaatgtggc tttttcttta taatcccact
    10681 gaacttcaat aaatctctgg tcttcagaat cgcttaggtc atgaatattg gtgcagtcag
    10741 ccctgtggac agagaccccc ctgccccttg taatataccc aactatggcg tcaccgggca
    10801 cagggttgca gcatttggag aatcgtattt ttatattgtc aatacctttt attataacgc
    10861 ctgatgcgtg cttctcaact ctctgaggag ttttttcagc cgttttttca agattttcga
    10921 ttttttcaat ttcaagctta tctttgtttt tctcaagata tatctgttta agcctgttaa
    10981 atatctgatt ttcggttatg cttccgtttc caacggcagc ataaaggttg ctggctgtat
    11041 gcatattgta tttatctgca actgttttaa tccagtcatc cttcagaagg tctcctactt
    11101 gaaagccctg ctttttaatt tctctttcta ctaattcctt gcctttatct tcattgaagt
    11161 ccttatcttt ttctttgaaa aattttctga ttttggattt tgcctggctg gatgctacaa
    11221 tttttagcca atcacgactt ggccccgcac tcgcctgaga ggttagtact tcaacctgat
    11281 cgcctgtttt cagctttgta tcaataggaa ccattctccc gttaaccttt gctcccacgc
    11341 acttgttccc cactgcagag tggactctgt aagcaaagtc tattggtgtg gaccctgtag
    11401 gcagatttac aacttcccct tttggagtaa atacaaaaac ctcatctgta taaaggtcaa
    11461 tttttaaagc ctccataaat tcctgagggt catttgtttc ctgctgccat tcaagcattt
    11521 gcctcagcca gtttaatttt tcctcatact ttgtggtttc atctattcct tctttgtatt
    11581 tccagtgggc agcaatacca tattcagcag tttggtgcat ttccaaggtt cttatttgga
    11641 tttcaaaggg ctccccttca ggtccgataa ctgtggtatg gagtgactgg tacatatttg
    11701 gtttgggcat ggcaacataa tccttgaatc ttccaggtac cggtttccaa agtgtatgaa
    11761 cagtccccaa tgctccatag caatccttta tattgtccac aataattcta atggcaatca
    11821 tatcgtagat ttcctcaaag gaccgatttt tgaagtacat ttttttgtaa atgctgtata
    11881 agctcttggg tcttcccttt atatctgctt ccattccctg ttctttaagc ttatcgtgaa
    11941 taacttcaat aatgtgggaa atatagcctt tccttttttc acttttttcc tggacctttt
    12001 taagtaaatc ttcatagccc tcagggtcga tgtattnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnacca tatttgcaat ttcttcgccg aattcctttt taatttcatc
    12181 aaaggatata ccggtatcct caacaacatc atggagaagt cctgcgcata tggtttgtac
    12241 atccatgtgc aagtcagcca atataagcgc aacattataa gggtgaacaa aatatctttc
    12301 accggaaaat cttgtttgtg catttgaatg tgccatctca gcatagttgt atgctttgac
    12361 cacaatttcc aaatcagcat tcggattgta tgattcgatt tgattaataa tattgcctaa
    12421 catttctcca cctgtgcctt acttttggca tctctggttt atttcacttt atttatttgc
    12481 cggcttaagc cggcataaat tgtgtcccca taccgttgca aatagggtag ggcatcttat
    12541 ttgtcgtatg ttaccagcga ttccaccttg tattctttca atttctctct tccctttaaa
    12601 tccttcagct ctatgaggaa accaagcccc acaacttctc ccccaagctt ttcaaccagc
    12661 tttgcagtag caaacatggt tcctccggta gccagaagat cgtcaataat caataccttc
    12721 tgtccttttt taatcgcatc cttatgtatt tccaaagagt ctgtgccgta ttccagatca
    12781 tattcatgtt taattgtttc tgcaggaagc ttccctggtt ttcttacagg tacgaatcct
    12841 gtccttaaca aatatgcaag aggtgttgca aatataaatc ctcttgattc aggtcctgca
    12901 acaatatcaa cttgaacatc cttaaacctc tctgccattt cgttgatgca ctcataaagc
    12961 gcctccgaat ccttcagcag ggttgtaatg tccttgaaac tgattccctc agccggaaag
    13021 ccctctataa ctcgaatctt ttcttttaaa tccatatttt ccctccatgc attatttttt
    13081 tatatacaat atttcatttt ttaaagcata ttgaatgttt tctgcctttt tcaacatgca
    13141 tattatttct tccgctgtta tttttgtatt atatctttta ttaagctttt ctacaagcat
    13201 aaatatatcc gtcctgatta ttttggattt tgatttatta atcaaaaact ccaatgtttt
    13261 cagttttccc gcatcaattt tgtaatccaa ttcttccctg tcttcgacat cttgcaaaat
    13321 aagctgtatg cttctgctgc cgttatataa attctcgctt aaggatacaa tgaaggattt
    13381 aggcatggaa agaacctttc caagcagtgt aattttaccg aatccgatta ccggaatgct
    13441 ttgcccttca ttagacagca taaagcttat atgttttccc tccggaccta ccttccggat
    13501 atttttcaat gacacgtctc ttaatgccaa aataggtttt tgattgcctg atccaaaagg
    13561 ctcaaagtta tttatctcat caaaaagcct taaattcata tcatttatgc caagtatggc
    13621 atctgctctc aggtagtatt cttcatcatc attggaacct atataattgt tgagttcatt
    13681 tgtgaaggag tttatatttt cttccaaaac agtaagacct gcagctaatt tatggcctcc
    13741 gtacttttcc agataagagt cacaagcatt gagtgattcg aatatgtcta catgttccaa
    13801 gcttcttgct gagcctttac ctgttccatc cttcaccgaa ataaccacac atggcttata
    13861 atatttttcg gtcagttttg aggcaacaat tccaaggaca ccttcatgcc agtcatagcc
    13921 gtgtatcact attacattgt ttttatatag aaagtccgat tcaatttttt tgactgcttc
    13981 gttgaatatt tcactttctg cttcttttct tgcattgttt aggctttcca gttccaaagc
    14041 aaggatttgg gcttcttcct catcctctgt aagcatgagt cttattgcct tttttgccgt
    14101 atccattctt cccgaggcat taatcctggg cgccagcata aaacctatat gataagattt
    14161 aatctccttg tcctttatac ctgaaacctg gattaatttt ttgattcctg caatccttgt
    14221 attatttatc ctgcttaatc cccggctagc aataatcctg ttgtccttca ccaggtccac
    14281 tatatctgca atggtgccga ggcaggcata ttccaggagc acatcttcga tatcatttat
    14341 tttcaagtat tcattcaagt gcgtcaaaaa tttaaaagat agtcctgctg cacataaatg
    14401 cttgttttcc gacggacaat ctttttgttt tggattaata atggcatatg catggggaag
    14461 ttcttccctg cactgatggt ggtcaattat tattacatct gtatcaagct ttgttatttc
    14521 atcaatcttt tcattttcag ctatgccgca gtccacggtt atcaacaggt caatagatat
    14581 ttcaccgctt tttagtctgt cgacaaagtc ggtgcttatg ccgtatccct cgctttccct
    14641 ttccggaaca taataatcaa cctgggcgcc tgtttttttt aataatacaa caaactggct
    14701 gatggatgtt actccatcaa catcgtagtc gccgtaaatt aaaatccgct cattgttatt
    14761 aattgcttcg ataaccctct tgcagccttt atctaaatct ttgtaatttt ttgcactttc
    14821 aaaatatttg aaatctgggt caagaaactg ttctatatcg tcaaatgaac tgatattcct
    14881 gtttaaaagt atttttgctg atgtttttga aagattaaat cttctgctga ttctttcaat
    14941 ttcatcatta ctatattcat tgactttcag tttcactgtt ttcattgtct atctgtatcg
    15001 tgccttcatc tgcaatttct acacggaatt tatcaatatt ttgaattttc agcatttcta
    15061 tttcctggtt ctttccggtc aattcttctt cctgaaattt gattttgtct tcaagtttag
    15121 ttactttttc gataaaaccg gtattttcat cctgcaatgc ttgtctttcc ttttcaagtg
    15181 catcaacttt tgcagtaaaa tcagcttttt ctttttccag tgtctttatg tttgtttcaa
    15241 tttgagcttt ttcaccttca agagttttga ttctgttttt taattcctta tactcggatc
    15301 ttttcttgaa ttgccttgtg gtacccagta ttaataccat aacagctcca aggatgagac
    15361 atatcatcat tattactgcc aggggcattt catatctggc gaaaaacaaa tccataggaa
    15421 caggcgtccc gttttgtatt gcaaaaattg caacaaaaat cgctattata agaattacta
    15481 taaaaccaat ctgcataaat aacactcctc tcctgttaaa ctaatttttt tatctacttg
    15541 tattatacat attcccgtgc ataattgcca ctctttttta gttatttaca caaaat
//
LOCUS       DAOPUT010000034        15586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3850_length_15586_cov_19.662546, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000034 DAOPUT010000000
VERSION     DAOPUT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15586
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3850_length_15586_cov_19.662546"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1560)
                     /gene="scfB"
                     /locus_tag="PKK75_04515"
     CDS             complement(223..1560)
                     /gene="scfB"
                     /locus_tag="PKK75_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HNZ82891.1"
                     /translation="MIHIFNIKELYFAVDTNSGLVHAVDKIIYDLLMNEKFKDENKYR
                     GLSGEYGEDSVKEALSEIKYLIDNKMLYTEEINYINKIKPVIKAMCLNMTHDCNLSCE
                     YCFASQGTYNGEKAYLSFETGKKAFDYLVKNSGNRRNLEVDFFGGEPLMNFNTIKKLV
                     DYGRSLEKEYNKHFRFTVTTNGVLLDEEKIDYINENMDNVVLSIDGRKETNDRMRKTI
                     NGKGSYDLIIDNFKKFINKRGSKDYFIRGTYTSYNLDFSEDVKHMRESGFDKISVEPV
                     TAKPEEKYALKDEHVDILKKEYEKLTEYFIKSNKTKDKKFQFFHFNIDLESGPCIYKR
                     SMGCGAGTEYVAVTPKGELYPCHQFVGQEQFIIGSVDEGITNHAVTDKFKGLSVNDKP
                     ACRDCWAKYFCSGGCHANAYNFNNNLGIPYKTGCELEKKRIECSIYIKAKHIQ"
     gene            complement(1703..1873)
                     /gene="scfA"
                     /locus_tag="PKK75_04520"
     CDS             complement(1703..1873)
                     /gene="scfA"
                     /locus_tag="PKK75_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HNZ82892.1"
                     /translation="MKYIKTLNKAKLTDNISVKGCGECQTSCQSACKTSCTVGNQKCE
                     YDLTRFLRPQGR"
     gene            complement(1942..2313)
                     /locus_tag="PKK75_04525"
     CDS             complement(1942..2313)
                     /locus_tag="PKK75_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HNZ82893.1"
                     /translation="MNLGKLLKYSLFLFILSLIIFFILTIYIYYFSNNPGIEIIYSFI
                     VPICIFAVSMLYSRSVHEKGLVRGIEIWIVYFIIVLLIKVLFRYPSEIRILYNGIILL
                     MSVLGGITGVNIKNKSFVKRN"
     gene            2590..5274
                     /locus_tag="PKK75_04530"
     CDS             2590..5274
                     /locus_tag="PKK75_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNZ82894.1"
                     /translation="MYYQKSIKQTCEELNTIQSTGLSQEEAKNRLLSDGPNTLVEKKG
                     KTKLQMFLAQLKDTLIYILFAAAAISLALNEITDAIIILLVVLLNAVIGMVQESKAEA
                     ALEALKNLSSPYAMVRRDGKVMEIPAGDLVKGDVVILEAGRIIPADLRLIQSINLKIE
                     ESALTGESVPVDKNADLVTGGEVGIGDRLNMAFSSTSVAYGRGEGVVVYTGMDTEIGK
                     IAAMINESTEELTPLQKRLNDLGKVLGIVAVVIVVAMFGIAVIQGRDIIEMFITAIAL
                     AVAAVPEGLTAVVTIVLALGVTRMVKVNTIVRKLPAVETLGAVSIVCSDKTGTLTQNK
                     MTVTKVYFNGEVKNTDDLSYENNKLFLDGFVLCNDASTANNARIGDPTELALLDMGDK
                     INITREGLEETNPRINEQSFDSARKLMTTVHKDQTGKVMSYTKGAMDILLKRCSKIYL
                     NGQTTDITEKHIENINKAATEMAKGALRVLALGIREDDDSAREEDLTFVGLVGMIDPP
                     RPEARDSVKTLKKAGIRTIMITGDHKDTALAIAKELGIADNEAQCIPGSELNELTQEQ
                     LNEKVCDLRVFARVSPEHKVMIVKAFKSNGSIVSMTGDGVNDAPSLKAADIGVAMGIT
                     GTDVAKGASDMVLTDDNFATIEKAVAEGRGIYANIKKTVLFLLSSNFGEVISMFTAIA
                     AGLAAPLQSIHILWINLITDSLPALALGVDPKDKDIMSDKPRDPQESLFAHGALPFTV
                     YNGFMIGALTLGAFLWPPVIHLQEAGMAVTLENIRFMLGQVLIINGAEWSVIEHAQTY
                     AFTTLGISQLFHAIGMRNYNKSLFKMNHTENLAMIGAFSAGLLLQVLVTEVPFLTEVF
                     ETSRLSLGEWVNLILLSMVPLLSHEIIVMGKKLLKKDA"
     gene            complement(5284..5613)
                     /gene="yajC"
                     /locus_tag="PKK75_04535"
     CDS             complement(5284..5613)
                     /gene="yajC"
                     /locus_tag="PKK75_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNZ82895.1"
                     /translation="MPQEYSGILMLVVFFAIMYFTMIRPQKKKEKETKAMRDSLSTGD
                     EVITIGGIHGRVVKVNDELVTLEMPFAKQRIEFSKWAIGSVVKKGKEKAVKEEIEETP
                     DDNEENQ"
     gene            complement(5635..6744)
                     /gene="tgt"
                     /locus_tag="PKK75_04540"
     CDS             complement(5635..6744)
                     /gene="tgt"
                     /locus_tag="PKK75_04540"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNZ82896.1"
                     /translation="MFKFELLKESGECRARLGKIYTDHGIIETPVFMPVGTKGTVKAM
                     TPEEMKDLEAQIILGNTYHLYLRPGHELIREAGGLHKFMNWEGPILTDSGGFQVFSLG
                     DLRKIKEEGVEFRSHIDGSKHFISPEKSMEIQNYLGSDIMMAFDECAPYPADYDYVKR
                     SMERTTRWAKRCKDYHKNTKKQALFGIVQGGVFKDLRIESAGQITDIDFPGYAVGGLS
                     VGEPRDLMCQVMDYTVELLPKDKPRYLMGVGSPDYLFEAVERGIDMADCVLPTRMARH
                     GAFLTSRGQLTIKNAKYRNDFSPIDENCSCYTCRNYSRAYIRHLFGEKEILGARLASI
                     HNLFFLINLMKGIRISIREDNFLKFKREFYENYGY"
     gene            complement(6752..7783)
                     /gene="queA"
                     /locus_tag="PKK75_04545"
     CDS             complement(6752..7783)
                     /gene="queA"
                     /locus_tag="PKK75_04545"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNZ82897.1"
                     /translation="MTNYKTSDFYYDLPERLIAQTPIKERDHSRLLVLDKNTGEIEHR
                     HFYDIIEYLNPGDCLVINDTKVMPARLFGNKKDTKSIIEFLLLKRIDDKRWETLVKPG
                     KKAKIGAEIVFEENLLTGKIVDILEEGNRIVEFEYKGIFEEILDKLGEMPLPPYIHEK
                     LQDKDRYQTIYARHLGSAAAPTAGLHFNTELLEEIEKKTDVARLTLHVGLGTFRPVKA
                     EYINDHTMHSEYYELNERDADKINTARDTGHKVICVGTTSVRTLETLADEKGRVKAQR
                     GWTNIFIYPGYKYRATDSLITNFHLPESTLIMLVSALAGKENIMKAYHEAVEKEYRFF
                     SFGDAMYIK"
     gene            complement(7780..9459)
                     /locus_tag="PKK75_04550"
     CDS             complement(7780..9459)
                     /locus_tag="PKK75_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNZ82898.1"
                     /translation="MKKILIKILTMAILLTLLFSEAAFGENDDIYVRVRIRYPRLFNE
                     QVSFEGYDEIVLYDENDELLSLNNNLILRIDTYYAGNYYLSDISQGLYGPCHALLRDE
                     IYSSYDEAYIKAEELEEEFNSAFYPYLIEDGYIIAGGNYADKGSAEDLADELNKEGYN
                     AESYNGNLENIITYNDKNQPVFMYDKDMTLYFSSYNDDEEYEMIKVDNRAYRGRIKLN
                     IIDDFKLLTINLVELENYLYGVVPNEMSSSWGMESLKAQALASRTYAVYSAKPIQIYD
                     MEDNQNSQVYMGYDCEKPSTSRAVDETRGEMIYYDGKLIQAFYHSTSGGMTESSENVW
                     YEDLPYLRGVNDEFSDNSGSPHNEWQTTYYTEDIIRLLNLNENKVDEVYGVQINKVSE
                     NNRVLECIFLTDAGEISYKKENARLLLGLKSSWFSIVNGNAFYYISRYFSGIENQEDD
                     GATSRGGILDIIIDNENDETDEMKYETGSIFGKYAISSDGEKKINREKLSFISSRGVS
                     VVDTNSDQYNFEGRGWGHGVGMSQYGAKQMAEEGYTYKEILKHYYTGVTIK"
     gene            complement(9440..10495)
                     /gene="ruvB"
                     /locus_tag="PKK75_04555"
     CDS             complement(9440..10495)
                     /gene="ruvB"
                     /locus_tag="PKK75_04555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNZ82899.1"
                     /translation="MFERENEIVAGTFNDHDEELELSLRPQKLPQFIGQNKVKEVLNI
                     FIESAKMRNQPLDHVLLSGPPGLGKTTLANIISNELGVNIRVTSGPAIERAGDLAAIL
                     TNLNENDVLFIDEIHRLNKVVEEIMYPALEDFALDIIIGKGPSARSIRLDLPKFTLIG
                     ATTRSGMLASPLRDRFGVMCNLDFYEPADLKEIVLRSAGIINIEIGEKGAEEIAKRSR
                     GTPRIANRLLKRVRDYAIVKSKGIIDLETADRALKMLEIDEIGLDRLDRRILNTIIDF
                     YKGGPVGLDTLAASIGEEKVTIEDVYEPYLLQIGYLNRTPRGRVVSELGYRHVKKTRK
                     KEDQINVLEDDYEKDFD"
     gene            complement(10497..11081)
                     /gene="ruvA"
                     /locus_tag="PKK75_04560"
     CDS             complement(10497..11081)
                     /gene="ruvA"
                     /locus_tag="PKK75_04560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNZ82900.1"
                     /translation="MISYIKGKIVKKGIDYLIIDNNNIGYYINTSFSTLEKVSEKEEA
                     TILTYMHIREDILALYGFLTSDEIDLFKKLITVNGIGPRAGLSVLSTYNANTVKEIII
                     MEDTGRLSKVPGVGKKTASKIILELKDKVGTVEELQGTDIVISSEMSDVAEALTALGF
                     GYAEVKKTLGNMNLSGKTENEIIKEALKNMNRQG"
     gene            complement(11090..11590)
                     /gene="ruvC"
                     /locus_tag="PKK75_04565"
     CDS             complement(11090..11590)
                     /gene="ruvC"
                     /locus_tag="PKK75_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNZ82901.1"
                     /translation="MLILGIDPGLAISGYGILNYQGNKFEVVDYGCILTESSEEFPKR
                     LKHLYDSYTSLIDMYMPEAVAIEELFYNKNAKTAMAIAQARGVHLLAAENKSVPLYEY
                     TPLQIKQGIVGYGRAEKIQVQEMVRVILKLKEIPKPDDAADGLAAAICHAHSLKFADK
                     FKITKY"
     gene            11796..12947
                     /gene="rodA"
                     /locus_tag="PKK75_04570"
     CDS             11796..12947
                     /gene="rodA"
                     /locus_tag="PKK75_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNZ82902.1"
                     /translation="MFKKESKLKRFDFILLITTILLCVYGLIIINSATMSKAAGSEPY
                     LKTQIIAFALGLAVLFVLVMIDYDIYGSFYVPIYVLTNLLLLYTLINPVEASEWGDVR
                     SWIKIGPVVFQPSEIAKFGVIISLSKFIDINRENISSPLILLKILLFASLPVALIVMQ
                     PDLGTALVFVFFISIMIYIAGIDRKYVLAVLIIGLILIIAGVIIFFQTMEDYTLGDDY
                     RFDRIVTFFYPELDPDDTGYQVIQSKTAIGSGMIYGRGLYQGVQNQLGYLPTKETDFI
                     FAVIGEELGLTGGLVLLCLYAILFYRLTKVAKNAANMFGSLIVTGITAMLLFHIFENI
                     GMTMGLMPVTGIPLPFISYGGTFMLVNMVSIGLCLAVGIKRGKIDLNDL"
     gene            complement(13012..14664)
                     /locus_tag="PKK75_04575"
     CDS             complement(13012..14664)
                     /locus_tag="PKK75_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNZ82903.1"
                     /translation="MKVVLAGTNEIIEAYENETLYQLLMRVHEDDFFKYLGAKVNNKL
                     KGLCSRDYKENDKIEFIDITHPDGHRIYVRTLSLIYIKACKEVYNNIDVSINHSLNKG
                     LYTELQYKQPVTKKHLNAIKHKMQEIIDAKKIITTNFLSIDKAREIFSAQDMMDKVEM
                     LKYWDKDSIRVYELDGYYDTFYGYLAPNTGFVNKFDLKLFYPGIILNYPTRESNFELP
                     EYIEQKKISRVFKEAEDWGEIMDVAYVGALNNKIAGNSIGDMIRINEALHEKKTAYIA
                     DEITGDKNIKIVLIAGPSSSGKTTFAQRLSIQLRVNGKKTYAISMDDYFIDRHLTPRD
                     ENGDLDFETINALDLDLFNEQLIDLMEGKEVSIPVYNFKEGKREYTREPVKLTDDHII
                     IIEGIHGLNDELTENIPQANKFKIYISALTQLNIDRHNRIATSDLRLLRRIVRDYTHR
                     GNNALKTIELWDSVVRGAEKYIFPYQENADAIFNSALVYELCVLRKYAEPIIMEIGED
                     SSFYSERQRLLKFLSYFLPVEDDSAIPNNSILREFIGGSCFR"
     gene            complement(14636..>15586)
                     /locus_tag="PKK75_04580"
     CDS             complement(14636..>15586)
                     /locus_tag="PKK75_04580"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ82904.1"
                     /translation="HMKEIRITNNEEGQRLDRFLKKYLNRANQSFIHKMIRKKNIKVN
                     GSKAEPDDVLKTDDTVQLYLADDTIDKFREKKTLKQTNIHFDTIYEDNNILVVNKPIG
                     LSTQPDMTGMKNLVDEIKAYLDAKEENISFTFKPAVCNRLDKNTSGLVVAAKNYDALK
                     QTNKAIRERKIKRFYMAKVHGIIKDSLELRDYLIKNDNKNMVKIINEQREDSKPVVTY
                     AHPLKTEGRYTWLEVEIETGRAHQIRAHLSSIGHPVAGDKKYGKKDNEKYQILHAYKI
                     VLDGFEGNLSYLNGKEIISDISDRKILEEGKNESSIGRNK"
BASE COUNT         4343 a         3201 c         2702 g         5340 t
ORIGIN      
        1 attatacata ttcccgtgca taattgccac tcttttttag ttatttacac aaaattttca
       61 tgacctgccc cattttcccg ggtcgttttc cggaaaaaca tatggcaggc cgggcacggt
      121 gcaatccctg ttttcacggt caaaggaagt gcacaaattg gttatgcaaa atgagaatgt
      181 ttattgagga gaggctccgc tgtctttcag tacagcaaca acttattgaa tgtgtttagc
      241 ttttatatat attgaacatt cgatacgttt tttctcaagc tcgcatcctg ttttgtaagg
      301 aatgcccagg ttgttattaa aattatatgc gttggcatgg catccaccgc tgcaaaaata
      361 ttttgcccag caatctctgc acgcaggttt atcatttacg gaaaggcctt taaatttatc
      421 tgtaacagca tggtttgtaa taccttcatc aacgctgcct ataataaatt gttcctgtcc
      481 cacaaactga tgacaaggat acaattcacc tttaggtgtt acggcaacat attccgttcc
      541 ggctccgcag cccatagacc ttttgtaaat gcagggaccg gactccaagt caatattaaa
      601 atgaaaaaac tgaaactttt tatccttggt tttatttgat ttaataaaat attcagttaa
      661 tttttcgtat tcttttttta atatatctac atgctcatcc ttaagagcat atttttcttc
      721 aggttttgct gtaacaggtt caactgaaat tttatcgaat ccggattctc tcatatgctt
      781 aacatcttcc gagaaatcca aattatagga agtgtatgtg cctctgataa aataatcctt
      841 gcttcctctt ttgtttatga attttttaaa attatcaatt attaaatcat agcttccttt
      901 gccgtttatg gttttacgca ttctgtcatt tgtttctttt ctaccgtcaa tgcttaaaac
      961 cacattgtcc atattttcat taatataatc tattttttct tcatccaaaa gaacaccgtt
     1021 ggttgtaaca gtgaatctga aatgtttgtt atattccttt tccaaacttc ttccgtaatc
     1081 aaccagtttt ttgattgtgt taaaattcat aaggggctct ccaccgaaga aatctacttc
     1141 taagtttcgt cggtttcccg agttcttcac caggtaatca aaagcctttt tcccggtctc
     1201 gaagctgaga tatgcctttt ctccattata tgtgccttgt gaggcaaagc agtattcaca
     1261 acttaaattg cagtcatgag tcatgttcag gcacattgct ttaattaccg gttttatttt
     1321 gttgatataa ttaatttctt cagtataaag cattttatta tcaattaaat actttatttc
     1381 tgaaagagct tctttaactg aatcttctcc atattcacca gataaccccc tgtatttatt
     1441 ttcatccttg aatttctcat tcatcaacaa atcatatatg attttgtcaa cagcatgtac
     1501 cagtccgctg tttgtatcaa ctgcaaaata cagctccttt atattaaata tatgtatcat
     1561 tttccctcca tcttatcatt taaagtaaaa tggggacaac attgtgtccc caagccttat
     1621 tacccagtct cattcaccaa aatatttgct ccctttggtc gcatattttg ggaattcgtt
     1681 gcggttgacc taccatagat ttttatctcc cttgcggtcg taaaaatcgg gttaggtcat
     1741 actcacactt ttgattcccg acagtacaag atgtcttgca tgctgactga catgatgtct
     1801 gacattcacc gcatcccttt acgcttatat tatctgttag cttagcttta ttaagtgttt
     1861 ttatgtattt cattacaatg acaacctcct tataagtaaa taaattatag cactgattga
     1921 ggttatttaa aagtgtttta tttaatttct tttaacaaaa ctcttgtttt ttatgtttac
     1981 tccggttatt ccccccagta ctgacataag aagaataata ccgttgtaaa gtattctgat
     2041 ttcagaagga tatctgaaca aaacctttat caataaaact attatgaaat atactatcca
     2101 aatttcaatt cctcttacca aacccttttc atgaacgctt cttgaataga gcattgaaac
     2161 tgcaaatatg catattggaa caatgaagct gtagattatt tcgataccgg ggttatttga
     2221 aaaataatat atgtatatag tcagtataaa aaagattatt aaagatagaa taaatagaaa
     2281 cagagaatac ttaagtaatt tacctaaatt catattacct cctccagcat gacttattaa
     2341 ataatattca tacaaattta aaaaagaaca taaaataatg cttgacaaaa atttctacat
     2401 ttgataatgt ttattttgct gaaagggagt agttaaaaaa tttccagtca acatcacgga
     2461 atatgtattc tggctggaga tcctcaagta actcaggtaa cgagactttt agtatatctg
     2521 gaatgttttt ggatatactg aaagtctttt ttattaaaca gccagcaaat aattctaaag
     2581 gagtaaataa tgtattacca aaaaagtata aaacaaacat gcgaagaatt aaacacgatt
     2641 caaagcaccg gtttaagcca ggaagaagcc aaaaataggc tgctgtcaga cggacccaat
     2701 acattggtag agaaaaaggg gaaaacaaag cttcagatgt tcttggccca gctgaaggat
     2761 acactgattt acatattgtt tgcagctgca gcaatatctt tggctctgaa tgaaataact
     2821 gacgcaataa ttattttatt ggtagttttg ttaaatgcag taataggaat ggttcaagaa
     2881 tcaaaagcag aagctgcact tgaagccttg aaaaatcttt caagccccta tgcaatggtg
     2941 aggcgcgacg gaaaagtaat ggaaatacct gcaggagacc tggttaaagg agatgtagta
     3001 atacttgaag caggacgaat catacctgcc gacttaaggc ttatccagtc tattaattta
     3061 aaaatagaag aatctgcctt aaccggagag tctgtgcctg tagataaaaa tgccgacctt
     3121 gtaaccggcg gggaagttgg aatcggagac cggctgaata tggccttttc ttccacaagc
     3181 gttgcatacg gcaggggtga aggagttgta gtatatacgg gaatggatac tgaaataggc
     3241 aaaatcgctg caatgataaa tgaaagcacc gaagaactga ctcctctgca aaaaagactc
     3301 aatgacttgg gaaaagttct gggaatagtc gcagtggtta tagttgtagc tatgtttgga
     3361 atagctgtta tccagggaag agacataatc gaaatgttta ttacagcaat tgcattagcg
     3421 gtagctgctg tgcctgaagg tcttacggca gttgttacaa tagtgctggc acttggtgtt
     3481 accagaatgg tcaaggtgaa taccattgtc cgcaagcttc ctgctgttga aacactggga
     3541 gcggtaagta ttgtatgttc agataaaacc ggtactctca ctcaaaataa aatgactgta
     3601 acaaaggttt attttaacgg tgaagttaaa aatacagatg acttgtctta tgaaaacaac
     3661 aaattatttt tagacggctt tgttctctgc aatgatgctt ccacagccaa caacgcaaga
     3721 atcggcgacc ctactgagtt agctctcctg gatatgggag ataaaataaa tattacaagg
     3781 gaaggtctgg aagaaacaaa cccgagaatc aatgagcagt cctttgattc tgcaagaaaa
     3841 ttgatgacta cagtccataa ggaccagaca ggcaaggtta tgagctacac caagggtgct
     3901 atggacatac ttttgaaaag gtgcagtaaa atatacttaa acggccaaac aacagatata
     3961 acagaaaaac atattgaaaa tataaacaag gctgccacag aaatggctaa aggagccctc
     4021 agggtattgg ctcttggtat cagagaagat gatgattcag ccagggaaga agatcttaca
     4081 tttgtaggct tggttggaat gatagacccg ccccgccctg aagccaggga ttcggtaaaa
     4141 acattaaaaa aggccggaat cagaacaata atgatcaccg gcgaccacaa ggatactgct
     4201 ttagcgattg caaaagaact tggtattgca gataatgaag ctcagtgcat cccaggcagt
     4261 gaattaaacg agctgactca ggagcaatta aatgaaaaag tatgtgactt aagagttttt
     4321 gcaagagttt cgccggaaca caaggtaatg atagtaaaag cttttaaatc caacggaagt
     4381 atagtctcaa tgaccggtga cggtgttaac gacgctccgt cattaaaggc agctgatata
     4441 ggagttgcca tgggtatcac cggtactgac gttgcaaagg gcgcatcaga tatggttctt
     4501 actgatgata attttgcaac tattgaaaag gcagttgcag aaggaagagg tatatacgcc
     4561 aacattaaaa agacagtttt attcttgctg tcctccaact ttggtgaagt tatatcaatg
     4621 tttacggcta ttgctgcagg cctggcagca cccttgcagt ccatacacat cctatggatt
     4681 aaccttataa cagactcgct tcctgcctta gctttggggg ttgacccgaa ggacaaagac
     4741 ataatgagtg acaaaccaag agaccctcag gaaagcttat ttgctcacgg agctttaccc
     4801 tttacggttt acaatggttt tatgattggt gccttaaccc tgggagcatt tttatggccg
     4861 cctgttatac atctccaaga agcaggtatg gcagtaacac ttgaaaatat aagatttatg
     4921 cttgggcagg tacttattat aaacggtgct gaatggtctg ttattgagca tgctcaaacc
     4981 tatgcattta caacattagg tatatcacag ctcttccatg caatcggtat gagaaattat
     5041 aacaaatccc tgtttaaaat gaaccatact gaaaatcttg caatgattgg tgccttctca
     5101 gcaggtttat tgctgcaggt acttgtaaca gaggttcctt tcctcactga agtgtttgaa
     5161 acaagccggc tttcactggg agaatgggtg aatttaatac tcttatcgat ggtaccgctc
     5221 ttatctcatg aaataatagt tatgggcaag aagctgttaa aaaaagacgc ttaggcgtct
     5281 tttttactgg ttttcctcat tatcatctgg agtttcttca atttcttcct taacagcttt
     5341 ttctttgccc ttcttaacta cgcttcctat tgcccatttg gaaaactcaa ttctttgttt
     5401 ggcaaaaggc atttccaaag tgacaagttc atcgttcact ttaacaaccc ttccgtgaat
     5461 tccgcctatg gttattactt catcacctgt tgacaagctg tctctcattg ctttggtttc
     5521 tttttctttc tttttctgtg gtcttatcat tgtgaaatac attatcgcaa agaaaacgac
     5581 caacattaat ataccgctat attcctgtgg catgactcct cctgtgcatt atatttaata
     5641 tccataattt tcgtaaaatt ctcttttgaa tttcaggaaa ttatcttctc ttatacttat
     5701 tctaatacct ttcattaaat taatcaagaa aaataaatta tgtattgatg caagccttgc
     5761 acccagtatt tctttttcac cgaataaatg ccttatatag gctctcgaat aatttctgca
     5821 ggtgtagcag ctgcagtttt catcgatggg gctgaaatca tttctgtatt ttgcattttt
     5881 aattgtaagc tgtcccctgc ttgttaaaaa tgcaccgtgt cttgccatcc tcgtaggcag
     5941 tacgcagtca gccatatcta ttcctctctc aacagcctca aataaataat cagggcttcc
     6001 cacccccatc aaatatcttg gcttatcctt aggcaaaagt tcaactgtgt agtccattac
     6061 ttggcacatg aggtctctgg gctcacctac gctcaaaccc cctaccgcat acccgggaaa
     6121 atcaatgtcc gtaatctgcc cggcactttc aatcctcagg tccttgaaca cgcctccctg
     6181 aacaattcca aacaaggcct gtttttttgt atttttgtga taatccttgc acctttttgc
     6241 ccacctggtg gttctttcca tggacctttt aacataatca tagtctgcgg gataaggtgc
     6301 gcactcatca aaagccatca ttatgtcaga acccagataa ttttgtatct ccatggattt
     6361 ttcagggctt ataaaatgct ttgagccgtc gatgtgagac cggaactcaa ctccttcttc
     6421 ttttattttt cttaagtcac caagtgagaa aacctggaat cctccgctgt cggttaaaat
     6481 aggtccttcc cagttcataa acttgtggag ccctccggct tctcttatca attcatgacc
     6541 cggtcttaaa taaaggtgat aggtgttccc cagtataatc tgagcctcga ggtccttcat
     6601 ttcttcaggt gtcattgcct tgactgttcc ctttgttccc acaggcatga aaacaggggt
     6661 ttctataata ccgtggtctg tataaatttt accaagtctt gccctgcatt ctccggattc
     6721 ttttaataat tcaaacttaa acataattca cctattttat atacattgcg tccccgaagc
     6781 tgaaaaatct gtattctttc tctactgctt catggtacgc cttcataata ttttcctttc
     6841 ctgccaatgc gcttaccagc ataattaatg ttgattccgg aagatgaaag ttagtgatta
     6901 aagagtctgt cgctctatat ttataaccag gatatatgaa aatatttgtc catccccttt
     6961 gagccttcac tcttcctttt tcatctgcaa gagtttccag tgtcctgaca ctggtagtgc
     7021 ctacacatat cactttgtgc cctgtatccc tggcagtgtt tattttatcg gcatcccttt
     7081 catttagctc atagtattcg gaatgcattg tatgatcgtt gatatattca gcctttacag
     7141 gtctgaatgt tccaagtccc acatggaggg taagccttgc cacatctgtt tttttctcta
     7201 tttcctccag gagctctgtg ttaaaatgaa gtcctgctgt aggagcagcc gcagagccta
     7261 aatgccttgc ataaatagtc tgatatctgt ccttatcctg aagtttttcg tgaatatagg
     7321 gaggaagggg catttctcct aatttatcca atatttcttc aaatatgccc ttgtactcaa
     7381 actctacaat tctgttgcct tcttcaagaa tatcaacaat ttttcctgta agcaaatttt
     7441 cttcaaaaac aatttcagct cctatttttg cctttttacc aggtttaacc aatgtttccc
     7501 atcgtttatc atcaattctt ttaagtagta gaaattctat tattgatttt gtgtcttttt
     7561 tatttccaaa taatcttgca ggcataacct ttgtgtcgtt aattaccaaa cagtcacctg
     7621 gatttaagta ttcaataata tcataaaaat gcctatgctc tatttctcct gtatttttgt
     7681 caaggaccag aagccttgaa tggtctcttt ccttaatggg cgtctgagct atgagccttt
     7741 caggcaggtc gtaataaaag tcgctggttt tataatttgt catttaatag taactccggt
     7801 ataataatgt tttaatattt ccttgtaggt atatccttct tccgccattt gcttagctcc
     7861 gtactggctc attcctactc catgtcccca gcctcttcct tcaaaattat attggtcgga
     7921 attggtatct actacggata ctcctctgga acttatgaag gataatttct ccctgtttat
     7981 tttcttttct ccatctgagc ttattgcata cttcccaaaa atactccctg tttcatattt
     8041 catttcatcg gtttcatcat tttcattatc tattataatg tcaagaattc cccctcttga
     8101 tgtggcccca tcgtcttcct gattctcaat tccggaaaag taccggctta tataataaaa
     8161 tgcatttccg tttactatag agaaccagga gcttttcaat cccaaaagaa gcctcgcatt
     8221 ttctttctta tatgagattt cccctgcatc ggttaaaaat atacattcaa gaaccctgtt
     8281 attctcagag actttattta tctgaacccc gtacacttca tccaccttgt tttcgttcag
     8341 atttaaaagt cttattatgt cttctgtgta atatgtagtc tgccattcat tgtggggtga
     8401 gcctgaatta tccgaaaatt catcatttac acctcttaaa tagggcaggt cttcatacca
     8461 gacattttca gagctttctg tcatcccgcc gctggttgaa tgataaaagg cctgaatcag
     8521 cttgccgtca tagtatatca tttctcctct tgtttcatcc acagctctgc tggtagaagg
     8581 cttttcacaa tcatagccca tatagacctg gctgttttga ttgtcttcca tatcataaat
     8641 ctgtattggt ttagcgctgt aaaccgcata ggtccttgaa gccaaggcct gggcctttaa
     8701 agactccatc ccccaggatg aagacatttc gtttggaaca acaccataca ggtagttttc
     8761 aagctcaact agatttattg ttaaaagctt gaaatcatca attatattga gcttgattct
     8821 tcccctgtag gctctgttgt caacctttat catttcatat tcttcatcat cattgtatga
     8881 tgaaaaataa agagtcatgt ccttgtcata cataaataca ggctggtttt tgtcgttgta
     8941 tgtaattatg ttttccagat tgccgttata tgattctgca ttgtaacctt ctttgtttaa
     9001 ttcatctgct aaatcttccg cagatccttt atccgcataa tttcccccgg ctataatata
     9061 cccgtcttca attaaataag gataaaatgc agagttaaat tcttcttcta attcttctgc
     9121 ttttatataa gcctcatcat aggaagaata gatttcatcc cgcaggagtg catgacaggg
     9181 accatataaa ccttggctta tgtccgataa ataataattt cctgcataat atgtatcaat
     9241 tcttaaaata aggttgttat tcaggcttaa cagctcatcg ttttcatcat ataaaacaat
     9301 ttcatcatat ccttcaaaag atacctgttc attgaaaagt ctcggatatc ttattcttac
     9361 cctgacataa atgtcatcat tttcgccgaa ggctgcctca gaaaacaaaa gagtcagcag
     9421 tattgccatg gttagtattt taatcaaaat ctttttcata atcatcctct aacacattaa
     9481 tttggtcttc tttttttctg gtttttttaa cgtgtctgta acccagctcc gaaacaaccc
     9541 ttcctctggg tgtccggttt aaatacccta tctgcagaag atatggctca tatacatcct
     9601 ctattgtcac cttttcctcc ccaatggagg ctgccagggt gtcaagacct acaggtcccc
     9661 ccttgtaaaa gtcaatgatg gtgtttaaaa tcctcctgtc cagtctatcc agtcctattt
     9721 catcaatttc aagcatctta agtgccctgt ccgctgtttc taagtctatt atccccttgc
     9781 tttttacgat tgcataatct ctgacccttt ttaaaagtct gtttgcaatt ctcggggttc
     9841 ctcttgaacg ttttgcaatt tcctctgccc ccttttctcc aatttcaata tttataatac
     9901 ctgcagacct gagcacaatt tcttttaagt ctgccggctc atagaaatcc aaattgcaca
     9961 taaccccgaa cctgtccctt aatggggaag ccagcatacc cgaccgggtt gtcgccccga
    10021 tgagggtgaa ttttggcagg tcaagcctta tggacctagc tgaaggaccc ttccctatga
    10081 taatgtcaag agcgaagtct tcaagtgcag gatacatgat ttcttccacg accttattaa
    10141 gtctgtgaat ttcatcgatg aacaaaacat cattttcatt taaattggtc agtatagccg
    10201 ccaggtctcc tgccctttcg attgcaggtc ctgaagtcac ccttatattg actcccagct
    10261 cattggaaat tatattggct aaagtagttt tcccaagtcc cggaggtccc gaaaggagca
    10321 catggtcaag aggctggttt ctcattttgg cagattcaat aaatatattt aaaacttcct
    10381 ttaccttgtt ctgccctata aactgaggca gcttctgagg tctcaagctc agctccaatt
    10441 cttcatcatg gtcgttaaat gttcctgcaa ctatttcatt ttccctttca aacatattat
    10501 ccctgcctgt tcatattttt tagcgcttct ttaattattt cattttcagt cttgcctgac
    10561 agattcatat tccccagtgt ctttttaacc tcggcatagc caaatcccaa agctgttaaa
    10621 gcttcagcca catcagacat ttcactgctt atgacaatat cggttccttg tagttcttct
    10681 actgttccaa ccttgtcttt cagttccaag attattttac ttgctgtctt tttgcctaca
    10741 ccgggaacct ttgacaatct gcctgtatct tccattatga taatttcttt tactgtattt
    10801 gcattatagg ttgataatac tgacagtccc gcccttgggc ctattccgtt aacagttatt
    10861 aatttcttaa agagatctat ttcatcggaa gttaaaaaac cgtacaaggc aagaatatct
    10921 tcccttatat gcatatatgt aagtatagta gcctcttctt tttcactcac cttttccaat
    10981 gtggaaaaag aagtatttat gtagtaacct atgttgttgt tgtctattat taagtaatcg
    11041 attccttttt tgacaatctt gccttttatg tagcttatca ttttatccct caatatttag
    11101 taattttaaa tttatctgcg aatttcagtg aatgggcatg gcagattgct gccgccagac
    11161 cgtcggctgc atcatcgggc tttggaattt cttttaactt aagtattacc cttaccattt
    11221 cctgaacctg tattttttca gctcttccgt atcccactat tccctgtttt atttggaggg
    11281 gtgtatactc ataaagggga acacttttat tttcagccgc taaaaggtgg actccccttg
    11341 cctgggcgat tgccattgcc gtctttgcat ttttattata gaacagttct tcgattgcaa
    11401 cagcctcagg catgtacata tctataagag atgtgtagct gtcgtacaaa tgcttaagcc
    11461 ttttaggaaa ttcttcgctg ctttcagtca gtatgcagcc ataatctacc acttcaaatt
    11521 tgttgccctg atagtttaat attccgtagc cggatattgc cagtcccgga tctatcccaa
    11581 gtataagcat cctcttccct ttccaaatct cttgatgttt aaaattatag catacatttg
    11641 ttctaatata aagaacataa ttcatgaaat atttgttttc cattatttta gtatgtggta
    11701 taatagaaac gaactaaaga tgaaatccgg cccgataccg gtgatggcag ttcttcgatt
    11761 catgttaacc gggttttact taaacggagt ttaatatgtt taaaaaggaa agcaaactta
    11821 aaaggtttga ttttatattg ttaattacaa cgattttatt gtgtgtttat ggattaataa
    11881 taataaacag cgctacaatg agcaaagcag ccgggagcga accctatttg aaaacacaga
    11941 taatagcttt cgcccttggc ctggctgtct tattcgtcct ggttatgatt gattatgata
    12001 tatacggaag tttttacgtc cctatttatg tactgaccaa tctgctactt ctgtatacct
    12061 tgattaaccc cgtggaagct tccgaatggg gtgatgtaag gtcatggatt aaaatagggc
    12121 ctgtagtatt tcagccttct gaaattgcaa agttcggggt aataatttct ctgtcaaaat
    12181 tcattgacat taacagggaa aatatcagca gccctttaat tttattgaaa atattattat
    12241 ttgcatcttt acctgtggca ttgattgtaa tgcagccgga cttgggtacg gctcttgtat
    12301 ttgtgttttt tatctcaata atgatctata tagccggcat agacagaaaa tatgtgcttg
    12361 ctgttttgat tatcggatta atattgataa ttgctggagt tattatattc tttcaaacaa
    12421 tggaggatta cacattaggc gacgattatc gttttgacag gattgtaacc tttttctatc
    12481 ctgaattaga ccctgatgac actggctacc aggtaataca gtcgaaaaca gccataggct
    12541 caggaatgat atacggaaga ggcttgtacc agggtgtgca gaatcaatta ggatatcttc
    12601 ccacaaagga aacggatttt atttttgctg tgataggcga agaattagga ctcaccggag
    12661 gtttggttct tttgtgcctc tacgcaatct tgttttacag acttacaaaa gtagccaaaa
    12721 atgccgccaa tatgtttggc tcactaatag tgacaggcat caccgccatg cttttattcc
    12781 atatttttga aaatatcgga atgacaatgg ggttaatgcc tgtaacagga attcctcttc
    12841 cctttataag ctatggaggc acgtttatgc ttgtaaacat ggtatccata ggactgtgtc
    12901 tggcggtagg aataaaaaga ggaaaaattg atttaaatga tctgtaagag gtgtggggac
    12961 acacctcctc ttttgggaga gtctctgcgg gcaggaagaa tgtccccaat tttatctaaa
    13021 gcagcttccc ccgataaatt cccttaatat tgaattgtta ggtattgcac tatcatcttc
    13081 aacgggaaga aaatagctta aaaacttaag aagcctttgt ctttcggaat agaaactgct
    13141 gtcttcacca atttccatga taatgggctc cgcatatttt cttagaacac atagctcata
    13201 aaccagggcg gaattaaaaa ttgcatcagc attttcctgg taagggaaaa tatacttttc
    13261 agcacctctt actacactat cccacaactc tatggttttt aaggcattgt tcccccggtg
    13321 ggtgtaatct ctcactatcc gtctcaaaag cctcaaatca gaggttgcaa ttctgttatg
    13381 acggtcaatg ttaagctgtg tcaatgcgct gatataaatt ttaaacttgt tggcttgagg
    13441 aatattctct gtaagttcat cattgagccc gtgaattcct tcaattatta taatatgatc
    13501 atctgttaat tttacaggtt ccctggtata ttccctttta ccttccttga aattatatac
    13561 cggtattgaa acttcctttc cttccatcaa atcaataagc tgctcattaa aaaggtccaa
    13621 atccaaggca ttgatagttt caaaatccaa gtctccattt tcgtcccggg gagttaagtg
    13681 cctgtcaata aaataatcat ccatggaaat ggcataggtt tttttgccgt ttaccctcag
    13741 ctgaatcgat aatctttgtg caaaggttgt tttccccgag gaggagggtc ctgcaatgag
    13801 aactattttt atatttttgt ctccggtaat ttcatcagca atatatgctg tctttttttc
    13861 gtgaagggct tcgttgattc ttatcatgtc tccgattgaa ttgccggcga ttttgttgtt
    13921 caaagcacca acatacgcca cgtccattat ctctccccaa tcctcggctt ccttgaatac
    13981 tctggagatc ttcttttgct caatatattc gggaagctca aaattgcttt cacgggtagg
    14041 gtaattcaat atgattccgg gataaaacaa ttttaagtca aacttattga caaatcctgt
    14101 atttggagca aggtatccgt aaaaggtatc ataatagccg tccagttcat aaacccgtat
    14161 ggaatccttg tcccaatact tgagcatttc taccttatcc atcatatcct gagccgagaa
    14221 tatttctctt gccttgtcaa tacttaaaaa attagtggta attatttttt tcgcgtcaat
    14281 aatttcctgc attttgtgct ttatggcatt aaggtgtttc ttagttaccg gttgcttata
    14341 ttgaagctcc gtataaagcc ccttgttaag tgaatggttt atggaaacgt ctatattatt
    14401 gtatacttct ttgcatgctt taatgtatat tagtgacaat gtccttacat aaatcctatg
    14461 tccgtccggg tgggttatat ctataaattc aattttgtca ttttctttgt aatccctgct
    14521 gcagagtccc ttcagtttat tgtttacctt agcacccaaa tacttgaaaa agtcatcttc
    14581 gtgaaccctc atcagcaatt ggtacaatgt ttcattttca tatgcttcaa ttatttcatt
    14641 tgttcctgcc aatactactt tcattctttc cctcctctaa aatttttcta tcacttatat
    14701 ctgatataat ttcctttccg tttaaataag ataaattccc ttcaaatcca tcaagcacaa
    14761 ttttatatgc atggaggatc tgatatttct cattatcctt tttaccgtat tttttatccc
    14821 ctgcaaccgg atgtcctatt gaagagaggt gggcgcggat ttgatgagct ctgcctgttt
    14881 caatttccac ttcaagccat gtgtaccttc cttctgtttt cagaggatgt gcataggtta
    14941 cgacaggttt tgaatcttcc ctttgttcgt ttataatttt taccatgttt ttattgtcgt
    15001 ttttaataag gtaatcccta agctccagac tatcctttat gatgccatga acctttgcca
    15061 tatagaatct tttgattttt ctttccctta ttgccttatt tgtctgtttg agggcatcat
    15121 agttttttgc agctactaca agccctgatg tatttttgtc caagcggttg cacacggcag
    15181 gcttaaatgt aaagcttata ttttcttctt tagcatccaa atatgccttt atctcgtcaa
    15241 ccagattttt cattcctgtc atatccggct gagtgctcag gcctatgggt ttattcacca
    15301 ctaaaatatt attatcctca tatattgtgt caaaatgaat gttggtttgt ttcaatgttt
    15361 ttttctctct gaatttatct attgtgtcat cagctaaata aagctgaact gtatcatctg
    15421 tttttaaaac atcatccggc tctgcttttg agccgttaac ttttatattc tttttcctga
    15481 tcattttatg tatgaatgac tgatttgccc tattcagata tttttttaag aaccggtcca
    15541 atctttgacc ttcttcatta ttagtaattc ttatttcttt catgtg
//
LOCUS       DAOPUT010000035        15412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3903_length_15412_cov_19.311128, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000035 DAOPUT010000000
VERSION     DAOPUT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_15412_cov_19.311128"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1505
                     /gene="rsxC"
                     /locus_tag="PKK75_04585"
     CDS             174..1505
                     /gene="rsxC"
                     /locus_tag="PKK75_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNZ82905.1"
                     /translation="MSTKLFTFRGGVHPPQGKSFTQNVPIEEAPAPDIVVIPLSQHIG
                     APCEPLVAVGDKVKVGQKIGESKAFVSVPVHASVSGTVKKIESHQVPGGAKVKSIIIE
                     SDGKYEVHESVKPKGELEDLSREDILSIIKEAGMAGMGGATFPTHVKLSPPADKPIDT
                     LLVNGSECEPYLTADHRIMLEKPELVLLGTKAILKALGADKAYICIEDNKPDAIEILK
                     EIVGDEEKIEVVSMKTKYPQGDEKRLINAITGRIVPSGGLPMEVACVVENIGTVATIG
                     NVIKTGMPCIQRVVTVTGSAVENPKNLYVRLGTLFKDCIEACGGYKGEPGKIINGGPM
                     MGIAQYTDEVPVIKGTSGILVLNKEEANIPEPTNCILCGKCVDTCPVNLMPYKISRFS
                     LLKNFDGADEYHAADCIECGACSYICPAKRPLKESISVAKKEILTRRKKAK"
     gene            1518..2468
                     /locus_tag="PKK75_04590"
     CDS             1518..2468
                     /locus_tag="PKK75_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNZ82906.1"
                     /translation="MEIKLIGSSSPHIRSDETTQKLMLDVIIALVPALAASIFYFGIK
                     AAVLVLASVITAVATEYLCQRFMNRPITVMDLSAVVTGMLLAFNLPAEAPWWIAVVGS
                     AFAIGIVKQVFGGLGFNFLNPALGARAFLMASWPHHLSGGFIDPVTDAVSSATPLQLL
                     KGTTSGQLPSLWDMFIGNIPGVIGETSTILLLAGGIYLLYRGTIKWIIPVFYIGTVAA
                     IALVIDAGNLPYHILGGGLILGAFFMATDYATSPVTDKGKIIYAVGAGILTMFIRKLG
                     GYPEGVSYSILLMNILTPMIDKYVRPHVFGVATGGKKNEK"
     gene            2458..3030
                     /locus_tag="PKK75_04595"
     CDS             2458..3030
                     /locus_tag="PKK75_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNZ82907.1"
                     /translation="MKNNFIRLAGILFLIGAISAGVLAWLNDTTKDIIAQNEAMASMD
                     PAILEAVMPGSVMFYDYEDTALVETVKSENQKVINIMTAVDASGNELGKVVRTLSTVK
                     GFNGDMELYVGFTPDGQISGVSAISHQETEGLGSKTTLPAFTDQFKGIDGSSEIGEND
                     YDAVTGATYSSKSFLSAVNNAIRVYTDYLK"
     gene            3040..3636
                     /locus_tag="PKK75_04600"
     CDS             3040..3636
                     /locus_tag="PKK75_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNZ82908.1"
                     /translation="MNKLEIFKNGITTENPILVQQVGMCATLAITTSLINGIGMGVAV
                     IAVLTGSNIVISALRKYIPDEVRIPAFVIIIAAFTTIIDLLMHAYTFALYQALGVFIP
                     LIVVNCIILARAEAFAFKNGVVDSTIDGLGMGIGYLVAMILLSSIREILGNGTILGLR
                     IFPEAYEPMMMAIQPPGAFILLGLLIGLVNYLSIRKKA"
     gene            3647..4222
                     /locus_tag="PKK75_04605"
     CDS             3647..4222
                     /locus_tag="PKK75_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HNZ82909.1"
                     /translation="MELVSLFITSFIVNNIIFMQFLGTCPFLGVSKKIDTAAGMGIAV
                     IFVITLASIITYIIHYGILVTLGLEYLQTIVFILIIAALVQFVEMFLKKSAPGLYQAL
                     GIFLPLITTNCAVLGVAINNIIYEYTFVESLVYSVGISSGFLLAIVLFAGVRDKVDYA
                     DLPESLKGSAISLITAGLMSVAFLGFSGLQL"
     gene            4233..5042
                     /locus_tag="PKK75_04610"
     CDS             4233..5042
                     /locus_tag="PKK75_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNZ82910.1"
                     /translation="MVIVKAVAVTGGIGIVCGILLAFAAKLFHIDVDQRITDVRNALP
                     GANCGACGYAGCDAMAEAIASGKAPVNGCPVANAQAHAKIADIMGTTSEEGEKKAAHV
                     LCQGCDSLASKKYEYHGVKDCKAAAAVQGGDKSCDRGCLGYGNCVEVCDFDAIGIVDG
                     IAVIDKEKCTSCGKCIEECPKAVIEFVPYKNSVIVGCNNKDFGKAVKDVCKIGCIGCK
                     ICEKNCPFDAIHVTNNLARVDYDKCTHCMICVEKCPTKSIQGDLTIKDQAV"
     gene            5227..5865
                     /locus_tag="PKK75_04615"
     CDS             5227..5865
                     /locus_tag="PKK75_04615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNZ82911.1"
                     /translation="MEALKSIEEELKGREIRLSSLDKDRTALIVVDMVNGFVYSGPLA
                     SARVAAKVENIVELNRKMKGYKKMFFLDTHSDDSKEFCSFPVHCIKGDKEAELIPELM
                     TQDSQGQETYYIEKNSTNGFHAEGFKEWINKYEDEVDNFIITGCVTDICVLQFSLTLK
                     SYFNEKNKEKRIIVPANMVETYDGGSHDGNLINLISLYNMHTSGIEVVDGVI"
     gene            5878..7335
                     /locus_tag="PKK75_04620"
     CDS             5878..7335
                     /locus_tag="PKK75_04620"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNZ82912.1"
                     /translation="MHYNNFDQRRLSMLMDFYELTMANGYFSNGMKDEIVYFDLFFRK
                     NPDGAGFSIVAGLEQVIQYIKQLRFCQEDIEYLRSKKLFTEEFLQYLKDFKFSGDIYA
                     IPEGTPVFPNEPLITVRAKTIDAQIIETMMLLTINHQSLIATKASRVVRAAKGKPIME
                     FGARRSQGYDAAIYGARAAYIGGVVGTATTLADEAFGVPALGTMAHSWVQMFENEYQA
                     FKVYAENYPDNCNLLIDTYNTVKSGIPNAIKISKEVLEPMGKRLKGVRIDSGDIAYLS
                     KKCRKMLNDAGLHDCAIVASNSLDEFIISDLLDQGAKIDSFGVGERLITAKSEPVFGC
                     VYKLVAIEKEGKIIPKIKISDNEEKITNPGYKKVWRLHDKKTHSPIADVITLADEIID
                     FSRPYTIFDEIHTWKKKRVTRFYAKNLQVPIFINGECVYESPSLQDIRTYCKKKVDDL
                     WGEVKRFTNPHFYYVDLSKDLWDCKQNLISQYSFK"
     gene            complement(7362..8552)
                     /locus_tag="PKK75_04625"
     CDS             complement(7362..8552)
                     /locus_tag="PKK75_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNZ82913.1"
                     /translation="MERMVGTVVRGLRAPIITKGDDIVKIAVDSVLKASKSEGFSLRN
                     KDVVGITEAVVARAQGNYANIDQIAYDVKRKFNYDTAGLIFPILSRNRFSICLKGIAR
                     SFKKIYLMFSYPSDEVGNPLVDYDLLDREEINPWTDILSEYEFRSHFKNTRHVFTGVD
                     YIQYYSDIIRESGCQVEVIFANNPLSILNYTRNVLTCDIHTRERSKRLLKSNGAEIVY
                     GLDDILNHSVEGSGFNEKFGLLGTNKATEDTVKLFPRDCQALVEKIQEKLFEETGKKI
                     EVMIYGDGAFKDPVAKIWELADPVVSPGFTEGLKGTPNELKLKYLADNQFSHLKGEEL
                     KKAISLLIMNKDRNLKDSMESQGTTPRRLTDLLGSLCDLTSGSGDKGTPIVYIQGYFD
                     NYSA"
     gene            8697..9242
                     /locus_tag="PKK75_04630"
     CDS             8697..9242
                     /locus_tag="PKK75_04630"
                     /inference="COORDINATES: protein motif:HMM:NF023172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HNZ82914.1"
                     /translation="MIKIGMRNIKTAISVFLCLLFFEAVNRGNALFACIAAVICVQNT
                     IVDSVKIGMSRIIGTMLGGLSAALVLYIINSFFDDRILIFIIPLGIIFLIQICVSINM
                     KQAVSICCVVYLVVVISKNHEEGYMLYTFNRVLDTSIGIIIALLVNKYVEVPKKLKTN
                     FKDVPAEQEEIKKETEKEEEI"
     gene            9384..9653
                     /locus_tag="PKK75_04635"
     CDS             9384..9653
                     /locus_tag="PKK75_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HNZ82915.1"
                     /translation="MTGADLGIILAYTAGIMLVFMTSWIFFKPLKFLGRILLNSLIGA
                     VILIIFNYFGQYTGVYIGVNEITALIIGILGVPGFIALLLVKLLI"
     gene            9722..9847
                     /locus_tag="PKK75_04640"
     CDS             9722..9847
                     /locus_tag="PKK75_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82916.1"
                     /translation="MWLEYILLFSIAMMFFSISVVLTVETVHTTNSISKKRNKKS"
     gene            9927..11372
                     /locus_tag="PKK75_04645"
     CDS             9927..11372
                     /locus_tag="PKK75_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ82917.1"
                     /translation="MEKLNIISGIKQYRSEVDTNFHMPGHKGKPEILGELGKNLNFFD
                     ITETIGTDNLHYPTGFLKDAMKYISGVYGSKKSYMVVNGTSGGIISAIMACTNPGDKI
                     LVQRDCHKSVYNACILGDLKLFYLYPEFNKKYGLNFSISLDKLDQMLTDNPDIKMVVL
                     TYPTFYGICFDVKKAAEIVHKHDKILMIDEAHGSHLHFCRDLLPPSAESSGADIVTQS
                     THKTMPCLTQGSILHICSDRVNVNNIETMLRMLQTTSPSYILMASIENAVHWMEEHGE
                     ERLKRNIEVFKRRTLELRNMGINVLEDDFLIGEGLYDFDATRAVISMSELGITGTELQ
                     DILRYKYKIQMEFADLQYTVGYVTATDEPEDINRLFDAVKEIYIEESKSKEKREVVQI
                     EAFPQLQHERKMRKAFYAENILLDMYDAIGKTAAEFIIPYPPGIPLVCPGEIIVQETI
                     DYVKIMLENGINVNGVNKNNQVRVVKWKEDL"
     gene            11354..11962
                     /gene="tmk"
                     /locus_tag="PKK75_04650"
     CDS             11354..11962
                     /gene="tmk"
                     /locus_tag="PKK75_04650"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNZ82918.1"
                     /translation="MEGRFIVLEGSDGSGKSTMAERIGKYFSEKGCSIEFTREPGGTK
                     ISEKIRDLILDRDNIEMDYRTEALLYAASRAQLVSEKIIPWLKAGKVVISERYVYSSL
                     VYQGLGRGLGIDEVKRINDFAIAGVKPDLVILLDVEPEKALKRKLSIDGGDRLENEDI
                     SFHKSVYEGYKELSSHFPEIIVIDGERTIDEIFKDIKALLTI"
     gene            11974..12306
                     /locus_tag="PKK75_04655"
     CDS             11974..12306
                     /locus_tag="PKK75_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNZ82919.1"
                     /translation="MKLITIIVQNDDANKLISALRENGFSSTKMSSTGSFLSSGNTTV
                     ISGIEDDKVDDAIEIIRKICRTKKKVTAAIPPSSFSTVGGYLPQTIEVTMGGAVVFVT
                     PVDRFEKI"
     gene            12316..13287
                     /gene="holB"
                     /locus_tag="PKK75_04660"
     CDS             12316..13287
                     /gene="holB"
                     /locus_tag="PKK75_04660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNZ82920.1"
                     /translation="MLYSQTAGQEKIKTSLIKSINNKQVSHCYLFEGPEGMGKYKLAL
                     VFAQSLLCLNFSMEPCNECKSCIKVNSYNHPDLHIINEDGKSIKREDVDELVNSIYKR
                     PYEAKRKVYIIKDAHLMTPQAANTFLKTLEEPPGDTVMILLTVNSNLLLPTLVSRCQE
                     VKFRNVSKKTIKSYLKGYDTDSVDLAASYSKGILNKAVDIIEGKDDILKKRQEIIKVF
                     DRIINSDSEIIYELENYFEVEKDNIDLIIEIMIMWIRDVIFVNGNMEDLVINKDYMPL
                     LRNHGKNMKRDSDIVEFLQNTSDNIKSNVNYKLAVDNMLLKIQEVFK"
     gene            13284..14174
                     /locus_tag="PKK75_04665"
     CDS             13284..14174
                     /locus_tag="PKK75_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HNZ82921.1"
                     /translation="MIKVVGVRFKKAGKIYYFDPEKKWINNGDFVIVETIRGIEYGQV
                     VVGPKMVREENIVQPLKKVLRLATEEDIRINSENKEKEKEAFDICSKKIEEHGLEMKL
                     IDIEYTFDNNKVIFYFTADGRIDFRELVKDLAAIFRTRIELRQIGVRDEAKMIGGIGP
                     CGRHMCCSSYLGEFYPVSIKMAKEQKLSLNPAKISGICSRLMCCLNYEHLVYEENIKK
                     LPDIGDKVQVAGTNKTGIVVDINPLFVTAKVNVEKSDGTIEAEEIEGDRLKVLEWGAS
                     KQHKVEDIDIDLDVLKELED"
     gene            14256..14426
                     /locus_tag="PKK75_04670"
     CDS             14256..14426
                     /locus_tag="PKK75_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ82922.1"
                     /translation="MAHKITDACISCGACEPECPVGAISAGDDKYVIDAETCIDCGAC
                     ASVCPVEAPQQD"
     gene            complement(14617..>15412)
                     /locus_tag="PKK75_04675"
     CDS             complement(14617..>15412)
                     /locus_tag="PKK75_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019688650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNZ82923.1"
                     /translation="ETHEFINIMIDRDLFDLETKTNKQAGGYCTFIYDYNSPFIFANF
                     NGTQDDVTVVTHEAGHAFQSYQSRHFDAPEFNFPTPEAAEIHSMSMEFITWPWMESFF
                     EEDIDKFKFFHLADSLCFIPYGVTVDEFQHIVYENPDMTPDERKKAWRDIEKKYTPYK
                     DYDDNEFLNKGTYWFKQGHIFALPFYYIDYTLAQICSYQYWLRFMADRETAWKDYLKI
                     CNIGGSQSFLDILKTGDLSSPFEEVTISQVASKIKEYLNNIDDMIM"
BASE COUNT         5191 a         2441 c         3233 g         4547 t
ORIGIN      
        1 aaacaggtaa atattacttt tcattattat tgctttattt gattaaaaat gataaaattt
       61 cacaaataaa taaaaaaagt gtttaatagt cgaaaacaat ctgttataat ctttatgttt
      121 gagattatgg cattttttat aatttattat tattaaattt acgaggtggt ataatgagca
      181 caaaattgtt tacattcagg ggtggagtcc atccccctca aggtaagtct tttacgcaga
      241 atgtacctat cgaagaagca ccggctcctg atattgttgt aattcctttg tcgcaacata
      301 taggggcacc ctgcgaacct ttggtagctg taggagataa agtaaaagtc ggacaaaaaa
      361 tcggtgaatc aaaagccttt gtttctgtac ctgttcacgc cagtgtttca ggaacagtaa
      421 aaaagattga gagtcaccaa gtccccggcg gagcaaaagt aaaaagcatt attatagaat
      481 ccgacggaaa atacgaagta catgaatcag ttaagccaaa aggagaatta gaggatctct
      541 ccagggaaga tatactttcc ataataaaag aagcaggcat ggcaggaatg ggaggagcaa
      601 ccttcccaac acatgttaag ctgagccctc cagcggacaa acctattgac acattgctgg
      661 ttaacggatc ggaatgcgaa ccttatttga ctgctgacca caggattatg ctggaaaaac
      721 ctgaacttgt actgcttggt acaaaagcta ttttgaaagc attaggtgcg gacaaggcat
      781 atatctgtat cgaagataat aagcctgatg caattgaaat actaaaggaa attgtcggag
      841 acgaggaaaa aattgaagta gtgtcaatga aaaccaaata tccgcaaggt gatgaaaaaa
      901 gattaataaa tgctattacc ggaagaatag taccttcagg cggactaccc atggaagtag
      961 cctgtgtagt ggaaaatatc ggtacagttg ctaccatagg caatgttata aagacaggca
     1021 tgccttgtat ccaaagagtt gtaaccgtta caggtagtgc cgttgaaaac ccaaagaacc
     1081 tttatgtaag attagggact ttatttaaag attgcattga agcgtgcggc ggctacaaag
     1141 gggagcccgg aaagataatt aacggcgggc cgatgatggg aattgcccaa tataccgacg
     1201 aagttcctgt aataaaagga acgtctggaa tattggtttt aaataaagaa gaagcgaata
     1261 ttccggaacc tacaaactgt attttatgcg gcaaatgtgt tgatacatgt cctgttaatc
     1321 taatgcctta taaaataagc aggttttctt tactgaaaaa cttcgacggg gctgatgaat
     1381 atcatgcagc tgattgtatc gagtgcggtg catgctcata tatatgccct gcaaagagac
     1441 cgcttaaaga atcgataagt gttgctaaaa aagaaatatt aacaagacgc aaaaaggcta
     1501 aataggagga aatatagatg gaaattaaat tgattggttc atcgtcgcct catattagat
     1561 ccgatgaaac aacacaaaag ctgatgcttg atgtaataat tgcattagtg cctgcattgg
     1621 cagcaagtat attttatttt ggaataaaag cagcagtatt agtattggcc tctgtaataa
     1681 cagcagttgc aacagaatat ctttgccaaa gatttatgaa cagacctata accgtaatgg
     1741 atttaagtgc ggttgtaaca ggaatgctgt tagcatttaa tttacctgca gaagctccct
     1801 ggtggattgc cgttgtgggt tctgcatttg caattggaat agtaaaacag gtatttggag
     1861 gattgggatt taacttttta aatccggcat tgggggcaag agcattctta atggcatcat
     1921 ggcctcatca tttatccgga gggtttattg acccggtgac ggatgcagta tcttctgcaa
     1981 cacctcttca gctgctgaag ggtacaactt caggtcaact cccctcatta tgggatatgt
     2041 tcattggaaa tattccgggg gttatcggag aaacttctac tatactatta ctggcaggcg
     2101 gaatttatct gctttacaga ggaacaatca aatggataat acctgtattc tatattggaa
     2161 cagttgcagc aatagcactg gtcatagatg caggcaatct gccttatcac atactgggcg
     2221 gagggttgat cctgggagca ttttttatgg caaccgatta tgcgacaagc cctgttaccg
     2281 ataaggggaa gattatttat gctgttggag cagggattct gacaatgttt ataagaaaat
     2341 tgggcggtta ccctgaagga gtatcttatt caatattatt gatgaatatt ttaacaccta
     2401 tgattgataa atatgttaga cctcacgtat ttggagtagc aacaggaggt aagaaaaatg
     2461 aaaaataatt ttataagatt agcaggtata ttatttttaa taggtgctat atcagcgggt
     2521 gttttagctt ggctaaatga tacaacaaaa gatataatag cccaaaatga ggcaatggca
     2581 agcatggatc ctgctatatt ggaagctgtt atgcccggct cagtaatgtt ttatgattat
     2641 gaagatactg ctttagttga aacagtaaaa agcgaaaatc aaaaggttat aaatataatg
     2701 actgcagttg atgcttccgg gaatgaactt ggcaaagttg tgagaacatt gagcactgtt
     2761 aaaggcttca atggggacat ggaattatat gttggtttta caccagatgg acaaatatca
     2821 ggagttagtg caatttctca tcaggaaact gaaggtttag gttcaaaaac tactcttcct
     2881 gcttttacag accagtttaa aggtatagat ggaagcagcg agattggtga aaatgattac
     2941 gatgccgtta ctggtgcaac atattcttct aaatcatttt taagtgccgt aaataatgcc
     3001 ataagagttt atacggatta tttgaaatag gaggatgaaa tgaataaatt agaaatattt
     3061 aaaaatggta taacaacaga aaatcctatt ttagttcaac aggttggtat gtgtgctacc
     3121 ttggcaataa ctacatcact tataaacgga ataggtatgg gagtggctgt aatagctgtt
     3181 ttgaccggtt caaacatagt tatatctgcg cttcgtaaat atataccgga tgaggttcgt
     3241 atacctgcat tcgtaataat tattgccgca tttactacaa ttattgattt attgatgcat
     3301 gcatacacat ttgcattgta tcaggcatta ggtgtattta ttcctttaat agttgttaac
     3361 tgtataatac ttgcccgtgc tgaagctttt gcttttaaaa acggagtagt ggattctacc
     3421 attgacggtt tgggaatggg tattgggtac ttagtagcta tgattttact cagctctatt
     3481 agagaaattt taggtaatgg tactatttta ggattaagaa tttttcctga agcatatgag
     3541 cccatgatga tggcaataca accgccggga gcttttattc tccttgggtt attgatagga
     3601 cttgtaaatt atctttcaat aagaaagaaa gcttaggagg caggaaatgg aattagttag
     3661 tttatttatt acatcgttta tagttaataa tataatattc atgcaattcc ttggaacttg
     3721 ccctttcctt ggggtttcca agaaaattga tactgcagca ggcatgggta ttgccgttat
     3781 attcgttata actttggcat ccattataac atatattatt cattatggta ttttggtgac
     3841 tttaggattg gagtacttgc agacaatagt attcatactg ataattgcag cattggtaca
     3901 atttgttgaa atgtttctca agaaatcagc accgggtttg tatcaggcat tgggaatttt
     3961 cctgcccctt attactacaa actgtgcagt attgggtgtt gcaattaata atattattta
     4021 tgaatacaca tttgttgaat cattggttta ttcagtaggt atatcctcag gcttcctgtt
     4081 ggcaatagta ttatttgcag gagtaagaga taaggttgat tatgctgatt tgcctgaatc
     4141 attgaaaggt tctgctattt cattgattac agcaggtctt atgtcagttg cattccttgg
     4201 tttttcagga ttgcaattat aggaggtaga aaatggttat agttaaagca gtggcagtta
     4261 ctggaggtat tggtatcgta tgtggtattt tattggcatt tgcagctaaa ctgtttcata
     4321 tagatgtaga tcagagaatt accgatgtaa gaaatgcttt gccgggagca aactgcggtg
     4381 cctgcggata tgcaggctgt gatgccatgg cagaagcaat agcatcaggc aaggctcctg
     4441 taaacggatg tcctgtggca aatgcccagg ctcatgcaaa aatagctgat attatgggta
     4501 caacatctga agaaggagaa aagaaggcag ctcatgtttt atgtcaggga tgtgacagcc
     4561 tggcatctaa aaaatatgaa taccatggag taaaagactg caaggcggcg gcagcagtcc
     4621 agggtggaga taagtcctgt gacaggggat gcctgggtta cggaaactgt gttgaagtat
     4681 gtgattttga tgctattggt atagttgacg gtatagcagt aatagacaag gaaaaatgca
     4741 cttcctgcgg taaatgcata gaagaatgtc caaaagcagt tatagagttt gttccttaca
     4801 aaaatagcgt aattgtagga tgcaacaaca aagacttcgg taaagctgtc aaggatgttt
     4861 gtaaaatcgg ctgcatagga tgcaaaattt gcgaaaagaa ctgtcctttt gatgcaatac
     4921 atgttacaaa taatttagcc agggtagact atgataagtg cacacattgc atgatttgtg
     4981 tggaaaaatg tcctacaaag tctatacaag gagacttaac aataaaagat caagcagtgt
     5041 agtgcaaatt tgatgcattt acctgcttat ggcctaactc atatgaataa agatctatcc
     5101 tgacaggata gatttttttc tatgacttaa tacctcaaga ttccctctgc attgacaaat
     5161 gttgttttaa gcactataat atataaatat tagatcttat aaaaaaatag aataaaggaa
     5221 gatgttatgg aagccctaaa aagtatagag gaagaattga aaggcaggga aatccggttg
     5281 tcatccctgg acaaagacag gacagccctt attgtagtgg atatggttaa cggttttgtt
     5341 tattccggac cgctggcatc agcaagagtt gcagcaaagg ttgaaaatat tgtggaatta
     5401 aacaggaaaa tgaagggtta taagaagatg tttttcctgg atacccatag tgatgattcc
     5461 aaggagtttt gcagctttcc ggtccattgc ataaaaggag ataaggaggc agaattaatt
     5521 ccggagctta tgactcaaga ttctcaaggc caagaaacat actacataga aaaaaacagc
     5581 accaacggtt ttcatgcaga aggattcaag gaatggataa acaaatacga ggacgaagtc
     5641 gataatttta ttattacagg ctgtgtaaca gatatttgcg tattacagtt ttcattaaca
     5701 ttaaaatcat actttaatga aaaaaataaa gaaaaaagaa ttatagtgcc tgccaacatg
     5761 gtagaaacct atgatggggg aagtcatgac gggaatctta ttaatttaat ctccctatac
     5821 aacatgcata ccagcggtat agaagtagtg gacggagtaa tatgagagga gtttattatg
     5881 cattataata attttgatca aagaaggtta tctatgctca tggacttcta tgagcttaca
     5941 atggcaaatg gatatttttc aaacggaatg aaggacgaaa tagtatattt tgatttgttt
     6001 ttcagaaaaa atccggatgg ggccgggttt tccatagtag caggattaga gcaggtaata
     6061 caatatataa agcagttaag attttgccag gaggatattg aatacctaag aagcaaaaag
     6121 ttatttacag aagaattttt acagtatttg aaggatttca agttttccgg agatatatac
     6181 gcaattcctg aaggaactcc tgtttttccc aatgaaccat tgattacagt cagggctaaa
     6241 acgattgatg ctcaaataat cgaaacaatg atgctgttga caataaacca tcaaagcttg
     6301 attgcaacta aagcaagccg tgtagtaagg gctgcaaagg ggaaacccat aatggaattt
     6361 ggcgccagaa gaagccaggg ctatgacgcc gctatatacg gtgccagagc tgcttatata
     6421 ggcggtgtgg taggaacagc cacaacattg gcagatgaag cctttggggt gccggccctt
     6481 ggaactatgg cacattcgtg ggttcaaatg tttgaaaacg aataccaggc atttaaagtt
     6541 tatgcagaaa attatcctga taattgcaat ttactgattg atacctacaa tacggtaaaa
     6601 agcggtatac caaatgcaat caaaatatct aaagaagtgt tggaacctat gggcaaaaga
     6661 cttaaaggag tcaggataga cagcggagac attgcatact taagcaagaa atgcagaaag
     6721 atgctgaatg atgcaggact tcatgattgt gcgatagttg catcaaacag tctggatgaa
     6781 tttattattt cagatttatt ggaccaaggt gcaaaaattg attccttcgg tgttggagaa
     6841 agactgataa ctgctaagtc agagccggta ttcggatgtg tttacaagct tgtggcaatt
     6901 gaaaaagaag gaaagataat tccaaaaatt aaaatatccg ataatgaaga aaaaatcacc
     6961 aaccctggtt acaagaaggt atggagactc catgacaaaa aaactcacag cccaattgca
     7021 gatgtaataa ccctggctga tgaaattatc gacttctcaa gaccctatac tatatttgat
     7081 gaaattcata cctggaagaa gaaaagagta acaagattct atgcgaaaaa tctccaggta
     7141 cctattttca taaacggaga atgtgtttat gaaagtccaa gcctgcagga tataagaaca
     7201 tactgcaaaa aaaaggtgga tgacctgtgg ggagaagtaa aaagattcac taaccctcac
     7261 ttttactatg ttgacttgtc taaagacctg tgggactgta aacagaattt aatatctcaa
     7321 tacagcttta aatagcataa aaaaagggcc aagccctttt tttatgctga ataattatca
     7381 aagtaacctt gaatatagac tatgggagtt cccttatctc cgcttcctga tgtcaagtcg
     7441 caaagggagc ctaagaggtc tgtaagtctt cttggggtag ttccctgtga ttccatgcta
     7501 tcttttaaat tgcggtcctt gttcataatt agtaaagata ttgctttttt aagctcttct
     7561 cccttaaggt gagaaaattg attgtctgcc aaatatttaa gctttaattc atttggagta
     7621 cccttgagtc cttccgtaaa tcccggagaa acaaccggat cagccaattc ccaaatcttt
     7681 gccacagggt ctttaaaggc tccgtctccg tatatcatga cttcaatttt tttgcctgtt
     7741 tcctcgaata atttttcctg aattttttca accaaggcct ggcagtctct agggaaaagt
     7801 tttactgtat cttcagtggc tttatttgtg cctaaaagtc cgaatttttc attgaacccg
     7861 ctgccttcta ctgaatgatt caaaatatca tccagaccgt atactatttc tgcaccgttt
     7921 gatttcaata atcttttgct tctttcccgg gtatgaatgt cgcatgttag aacattcctg
     7981 gtataattta aaatgcttag cggattatta gcaaatataa cttccacttg gcagccgctt
     8041 tctcttataa tatcagaata atattgtatg taatccactc cggtaaaaac atgtctggta
     8101 tttttaaagt ggctcctgaa ttcatattca gaaagtatat ctgtccaagg gttgatttct
     8161 tccctgtcca gaaggtcata atcaaccaat ggatttccaa cttcatcgga agggtagctg
     8221 aacatcagat agattttttt aaatgacctg gcaattccct taagacatat tgaaaatctg
     8281 tttctgctta aaatcgggaa tattaaacct gcagtgtcat aattaaactt tcgttttaca
     8341 tcatatgcta tttgatctat atttgcataa ttgccctgag ctcttgcgac tacagcctcg
     8401 gtgataccta ctacatcctt atttcttaat gaaaaacctt cactttttga agctttgagc
     8461 acactgtcaa cagcaatttt tactatgtca tcaccttttg taattatggg agccctcaat
     8521 cctctgacta ctgttcccac cattctttcc ataacagcct ccttgaagtt tgtatactta
     8581 tattataata cattttctta cttgtgtaaa atattattta ttactggaca aattcataaa
     8641 tattatttac aatatgtata attccaatta attcattaaa tatccggagg ataaacgtga
     8701 taaaaatagg aatgcgcaat attaaaacag caatttcagt ttttttatgt ctcttgtttt
     8761 ttgaagctgt taacaggggc aatgccttat ttgcctgcat agctgccgta atttgcgtac
     8821 agaatacgat agtggattct gtaaaaatag ggatgtcaag aattatagga acaatgctgg
     8881 gaggtttgtc ggcagccctg gtactgtata taatcaattc cttttttgat gacaggattt
     8941 taatttttat tattccctta ggaatcatat ttttgattca aatatgcgta tcaataaata
     9001 tgaaacaggc agtttctatt tgctgcgttg tatacctggt tgtagtgatt tccaagaatc
     9061 atgaagaggg gtacatgcta tataccttca acagggtgct ggatacttca atcggtataa
     9121 taattgcgct tttagtcaat aaatatgtag aagtacccaa aaaattaaaa acaaatttta
     9181 aagatgtgcc ggctgaacag gaagaaataa aaaaggaaac agaaaaggaa gaagaaattt
     9241 agcttaataa cctggttgac atacaggcta aaattgaaat tttattgata ttatacttaa
     9301 tgtatcgata tgacataaat acagggacaa cttcataaaa ttaataaacc gggttttccg
     9361 gattattaaa tatggaggtt gacgtgactg gagcagattt aggtataata ttagcttata
     9421 cagcgggaat aatgcttgtt ttcatgacat cctggatatt tttcaaaccg ctaaaatttt
     9481 tgggaagaat acttttaaac tccctcatag gagctgtaat actgattata ttcaattatt
     9541 tcgggcagta taccggtgtt tatataggag taaacgaaat taccgctctt attataggaa
     9601 ttttgggcgt accgggattt attgctttat tgctggttaa actattaata taaataagca
     9661 cttaagtttt attggacaat ggtattatgc tgttatataa tgtgttcagg aaggtgattg
     9721 tatgtggctt gaatatattt tactattttc aattgcaatg atgttctttt ccatatcggt
     9781 ggtacttacg gttgaaactg ttcataccac caacagtatt tcaaaaaaaa gaaataaaaa
     9841 atcataaatc aaaaggagct gaagcactaa tcccgaaaat ataaacagat gagaaattta
     9901 tacatcaata aacgggagat aaaaagatgg aaaaacttaa tattatttct ggtattaaac
     9961 agtatcgaag cgaagtagat acaaatttcc acatgccggg ccataagggc aaaccggaaa
    10021 ttttaggtga actgggaaaa aacctaaact tttttgacat tactgaaaca attggaacgg
    10081 ataacttaca ttatccaacc ggttttttaa aggatgcaat gaaatatatt tcaggagttt
    10141 acggcagtaa aaaatcatac atggttgtaa acggaacatc cggcggaatt atttcggcaa
    10201 ttatggcttg tacaaacccg ggagataaaa tactcgttca gagagattgt cacaagtcgg
    10261 tttacaatgc ctgcatattg ggagatttga aactgtttta cctatatccg gaattcaata
    10321 agaaatatgg tttgaatttc agcataagct tggataaact tgatcaaatg cttaccgata
    10381 atccggatat aaaaatggtt gtactgacat accctacatt ttacggtatt tgttttgatg
    10441 taaaaaaagc agctgaaata gtacataaac atgataagat tttgatgata gacgaagcac
    10501 acggttcaca tctgcatttt tgcagggatc tactgccgcc ttcagctgaa agctcaggag
    10561 cggacatagt tacccaaagc acccataaaa ccatgccctg ccttactcag ggatcaattt
    10621 tacacatatg ctctgacagg gtaaatgtca ataatattga aacaatgctg agaatgcttc
    10681 aaacaacttc cccatcctat attttgatgg catcaataga aaatgcagtt cattggatgg
    10741 aggagcacgg agaagagaga ttaaagagaa atatcgaggt atttaaaaga aggacattgg
    10801 aactgagaaa tatgggaatc aatgtgcttg aagatgactt tttaatcggc gaaggtttgt
    10861 atgattttga tgcaacacgt gctgttatca gcatgagtga gcttggaata acaggtactg
    10921 agcttcagga tatattaaga tataaatata aaattcaaat ggaatttgct gacttgcagt
    10981 ataccgtcgg ctatgttacg gcaactgatg aacctgaaga tataaacaga ctttttgatg
    11041 cagtaaaaga aatatatatt gaggaatcca aaagcaagga aaagagagaa gttgttcaaa
    11101 ttgaagcatt tcctcagctt cagcatgaaa ggaaaatgag gaaggccttt tatgcagaaa
    11161 atatacttct tgatatgtat gatgccattg gcaagacagc agcagaattt atcataccat
    11221 atcctccggg aatacccctt gtatgcccgg gagaaataat tgttcaagaa acaattgact
    11281 atgtaaaaat catgcttgaa aacggcatta acgtaaatgg cgtaaacaaa aataatcaag
    11341 tgagagttgt taaatggaag gaagatttat agtactggaa ggctctgacg gttcaggaaa
    11401 atctacaatg gccgagagaa tagggaaata cttcagtgaa aagggctgtt cgattgaatt
    11461 taccagggag ccgggaggta ccaaaatcag cgagaaaata agagacctga ttttggacag
    11521 agacaacata gaaatggact acagaactga agctttgctt tatgccgcgt cgagagcaca
    11581 gctggtatct gaaaaaataa ttccctggct taaagcaggg aaagttgtaa taagcgaaag
    11641 atatgtttat tcaagtttag tataccaagg ccttggaaga ggccttggta ttgatgaggt
    11701 aaagagaata aatgattttg ccatagcagg ggttaagccg gatctggtga ttcttttgga
    11761 tgtggaacct gaaaaagcat tgaaaagaaa gctttctatc gacgggggag acaggcttga
    11821 aaacgaggac atttcttttc acaaaagcgt atacgaagga tataaagagc tatcatcgca
    11881 ttttcctgaa attattgtaa tcgacggcga aaggacaatc gatgaaatat ttaaagatat
    11941 aaaagctcta ttaacaatat aaggagggtt cttatgaagc tgataactat aatagtgcaa
    12001 aacgatgatg ccaacaaact gatatccgca cttagagaaa atggtttttc ttccaccaag
    12061 atgtcatcaa ccggaagctt tttaagctca ggaaacacaa ctgtgatttc agggattgaa
    12121 gatgataagg ttgatgatgc tattgaaata ataagaaaaa tttgcaggac aaagaagaaa
    12181 gtcacagctg ccataccacc cagttcattc agcaccgtag gaggttattt accacagact
    12241 attgaggtta caatgggtgg agctgttgtg tttgtaactc ccgtagacag gtttgaaaag
    12301 atttaggggg aagctatgct ctacagccag acggcaggtc aggaaaaaat aaaaacatcc
    12361 ttgatcaagt caattaacaa taaacaggta tcccactgct atttatttga aggacctgaa
    12421 gggatgggta agtataagct tgcattagta tttgcccagt cactcttgtg cttaaatttt
    12481 agcatggagc cctgcaatga atgcaagagt tgcataaagg taaattctta caatcacccg
    12541 gatctgcata ttataaatga agatgggaaa agcattaaaa gggaggatgt tgacgaactg
    12601 gtaaactcaa tatacaaaag accctatgaa gcaaaaagaa aggtctatat aattaaagac
    12661 gctcatttga tgactcccca ggcagccaac acattcttaa aaaccttgga agagccgccg
    12721 ggggatacgg ttatgatact tttgacagta aattcaaatc tgcttcttcc aactcttgta
    12781 tccaggtgtc aggaggtcaa attcagaaat gtcagcaaga aaacgataaa gtcatattta
    12841 aagggctatg acaccgacag tgttgacttg gcggcaagct attcaaaagg aatactaaat
    12901 aaggcagttg acataattga aggcaaggat gatattttaa agaaacgcca ggaaattatt
    12961 aaagtttttg acagaattat aaattcggat tcggaaataa tttatgagtt agaaaattac
    13021 tttgaagttg aaaaagataa tatagattta ataatagaaa taatgataat gtggataaga
    13081 gatgtgattt ttgttaatgg caatatggag gaccttgtta taaacaaaga ttatatgccc
    13141 ctcttaagaa atcacggaaa aaatatgaaa agagattcag atattgttga atttctgcaa
    13201 aatacaagcg ataatataaa gagcaatgtt aactataaat tggcagtaga taatatgctt
    13261 ttaaagattc aggaggtctt taaatgataa aggtagttgg cgtaagattc aaaaaagccg
    13321 gcaagatata ctattttgat cctgagaaaa aatggattaa caatggagat tttgtaatag
    13381 tagaaacaat aagaggaatt gaatacggac aggttgttgt gggtccaaag atggtcaggg
    13441 aagaaaacat agtccagcca ttgaaaaagg tattgaggct tgcaacagaa gaagatataa
    13501 gaataaacag tgaaaacaaa gagaaagaaa aggaagcttt tgacatatgc tctaagaaaa
    13561 tagaagagca tggattggaa atgaagctta ttgatattga atacaccttt gacaacaata
    13621 aagtaatttt ttattttacc gcagacggcc gaatagattt cagggaattg gtaaaggatt
    13681 tggcggcaat attccgaaca agaatagaac ttaggcagat tggggttaga gatgaggcta
    13741 aaatgatcgg aggcatcgga ccttgcggaa ggcatatgtg ctgctcatca tatttaggag
    13801 aattttatcc cgtatccata aaaatggcaa aggaacagaa attgtccttg aatcctgcta
    13861 aaatatctgg aatatgctca cgcctcatgt gctgtctgaa ttatgaacac ctggtatacg
    13921 aagaaaatat aaaaaagctt cccgatatag gtgataaagt gcaggtagcg ggtacaaaca
    13981 aaacaggaat agttgttgat atcaatccct tatttgtaac tgcaaaagta aatgttgaaa
    14041 aaagcgatgg tactattgaa gcagaagaaa ttgaaggaga caggctgaaa gttttggaat
    14101 ggggtgcctc taaacagcat aaagttgaag atatagacat agatttggat gtattaaagg
    14161 aattggaaga ttaaataatt tattaaaaaa cactttgaaa tatttgcaaa tagtgctaca
    14221 ataaagatga ccatattggg ggaggtgaaa ttttaatggc tcataaaata acagatgcat
    14281 gcataagctg tggagcatgc gaacctgaat gcccagtagg cgcaatttca gccggggatg
    14341 acaaatatgt aatagacgct gaaacttgta ttgactgtgg agcatgtgcc agcgtatgcc
    14401 ctgtggaggc tcctcaacag gactgataaa aattaataaa agggacaaat tcttttattg
    14461 actggtcaat aaaagaatct gtccctttta taagttgacc tgatatggtt tttcaggttt
    14521 ttttctgaaa aatagcggca ggtcattcgc aacggacaac aaatttatgc gattaaacga
    14581 gcaaataaac ttgtgtccga aactgcgctg ccttccttac attatcatat catcaatatt
    14641 attcaggtac tctttaattt ttgatgcaac ctgacttata gttacttctt caaagggact
    14701 tgataaatct cccgtcttta atatatcaag gaaagattgg ctcccgccta tattgcatat
    14761 tttcaaataa tccttccatg ctgtttctct gtctgccata aaccttaacc aatattgata
    14821 tgaacaaatt tgggccaggg tgtagtcgat gtaataaaag ggcaatgcaa aaatatgtcc
    14881 ctgcttaaac cagtaggtgc ctttatttag aaattcattg tcatcatagt ccttataggg
    14941 agtatatttc ttttcaatat ccctccatgc tttttttctt tcatcgggag tcatatccgg
    15001 gttttcataa acaatatgct gaaattcgtc aactgtcact ccgtagggta taaagcacaa
    15061 ggaatccgcc aagtggaaga atttaaattt atcaatatct tcttcaaaga atgattccat
    15121 ccagggccat gttatgaatt ccatgctcat tgaatgtatt tctgctgctt caggtgttgg
    15181 aaaattaaat tcaggggcat caaaatgcct gctttggtaa gattgaaaag catggcctgc
    15241 ctcgtgggta acaactgtta catcgtcctg ggtgccgtta aaattggcaa atataaatgg
    15301 gctgttataa tcgtaaatga atgtgcagta tcctcctgcc tgtttattgg tctttgtttc
    15361 cagatcaaac aagtctctgt caatcattat gttaataaat tcatgagttt ca
//
LOCUS       DAOPUT010000036        15346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3923_length_15346_cov_17.377542, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000036 DAOPUT010000000
VERSION     DAOPUT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15346
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3923_length_15346_cov_17.377542"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKK75_04680"
     CDS             <1..365
                     /locus_tag="PKK75_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNZ82924.1"
                     /translation="ILASALIETGSRMDDLIFEEFKGTGNMEIHLDRKLSEKRVFPAI
                     DINKSGTRREELLLNNEEMQTIWQLRKAMSNFSTADVTERIIDGLIRTKTNDEFIKQA
                     AQILSIEKMKSEINDRYY"
     gene            502..702
                     /gene="rpmE"
                     /locus_tag="PKK75_04685"
     CDS             502..702
                     /gene="rpmE"
                     /locus_tag="PKK75_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNZ82925.1"
                     /translation="MKKDIHPNYKTVTVKCACGNTFETGSVLDEIRVEICSECHPFFT
                     GKQKFVDKGGRVDRFKKKYNME"
     gene            complement(827..1564)
                     /locus_tag="PKK75_04690"
     CDS             complement(827..1564)
                     /locus_tag="PKK75_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="selenophosphate synthase"
                     /protein_id="HNZ82926.1"
                     /translation="MEIRQIRDITIIDYDHKSYLGIACDSCGGIGYKEHDLVKASPQL
                     TAYHTGKVVLAELMSMGFTPFILADGLAVEMNNTGRQLIEGFNEVLSKLSFNVHLTGS
                     TEENIKTVQTSMGVTCIGFCSKDKLKFKKTKTGDICLLVGLPMVGNEVLQNPDKILDI
                     DDFEKLYLCDYINEMLPIGSRGIECELNDLLKDNKLKFEYESSISIDLKKSGGPSTSC
                     LVTLRMENLGIIKGIIKKPINVIGSFL"
     gene            1715..2656
                     /locus_tag="PKK75_04695"
     CDS             1715..2656
                     /locus_tag="PKK75_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ82927.1"
                     /translation="MKKELTPLRADLMLVVVAFLWGSSYVVTKGSIDIVGPVQIIFFR
                     FSIASALSLVFFGRYLKTSSKKEIKAGFMLGAMLCFGALFAFYGIRYTTVSKNSFIIS
                     TNVVVVPFLHWAAYKRKPTGVSLAAVFLMAVGLGFLTLDLSGSFKINIGDVISFGSVF
                     FYAAHVVLSDNYSKRYNPVSVNTYAMISVSFLSFAVLLLTNNLRINIPGKIMGDMLYL
                     SVFPTFLCYTMQLNAQKHTNATHAAIIISLESVFATVLAVLILEEKITLQMAAGFVFI
                     FISVLTSELGETILVRFKERQAGLLIEIPGNQTENKE"
     gene            2723..4402
                     /gene="dnaX"
                     /locus_tag="PKK75_04700"
     CDS             2723..4402
                     /gene="dnaX"
                     /locus_tag="PKK75_04700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNZ82928.1"
                     /translation="MSHQAIYRKFRPKIFDDVLGQEHVTRTLKNQIISGNTAHAYLFS
                     GIRGTGKTSTAKIFARAVNCLNNHEGNPCNECEICKSILDETNMDVIEMDAASNNGVD
                     DIRELRDKVKFLPVKSRYKVYIIDEVHMLSKGAFNALLKTLEEPPDHLLFILATTEPH
                     KIPATILSRCQRFDLKRIGSDIIVENMKKICSDLDIQYEEKALKLIAANSEGAMRDAQ
                     SILDRCISFNTRVDYETVINLLGTVNYQAVLKAADAIVDKNIEKTMILVDDILSSGKE
                     LTFFLDELIICFRNMLIIKTTNSSDNLMRISEDEANEIVELNKKITVDETVKIIEDLS
                     TAQAECKRAINPRILLETKLIKMLHNVNYVSKDDLVKRIESIENSLHEGFKTKDAERI
                     RENRTARINKEPDIKENIGGTKKTAGKEKPEIPPKENEEALDSKEDKSDEAVFNTILS
                     QWQAILKEIRSENAGLHAIVRESRLIEVKNKKAVFEFDPKFTFHISAANQPKNKDQFK
                     AALRRFLNEDCEVEFITKKEEKHIKQKTTIKDVYEDLKNEFGEIVEYKQED"
     gene            4405..4755
                     /locus_tag="PKK75_04705"
     CDS             4405..4755
                     /locus_tag="PKK75_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNZ82929.1"
                     /translation="MAKGRKMPMGMPGGGNMNNMMKQVQKMQKQMEDMQKELQQREVE
                     ATSGGGAVTVKVDGKKTLLELKIDPEVVDKDDVEMLEDMIVAAINEAFRKADEMTDSE
                     MKKITGGVNIPGLM"
     gene            4755..5351
                     /gene="recR"
                     /locus_tag="PKK75_04710"
     CDS             4755..5351
                     /gene="recR"
                     /locus_tag="PKK75_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNZ82930.1"
                     /translation="MEHYTAPVTKLIEEMAKLPGIGRKSAQRLAFHILDMKEADVVEL
                     AKAIVNAKKYTKHCQRCGNMTEGELCSICSSFKRDMSTICVVEDVRDIAAMERTKEYK
                     GVYHVLHGTISPLDGIGPDDINIKSLITRLNADVKEVILATNPTIEGEATAVYISRLI
                     KPMGIKVTRIAHGIPVGGDIEYADEVTLMRAMEGRREM"
     gene            5518..6567
                     /locus_tag="PKK75_04715"
     CDS             5518..6567
                     /locus_tag="PKK75_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006770869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HNZ82931.1"
                     /translation="MSKKNENEKESKFKSFLRKKNIKISVQRYLIEALGLMAYGLFAS
                     LLIGTILNTIGKKAGILFLTETIYPICLQMTGAAIGVAVAYGLKAPPLVLFASTITGT
                     AGNVLGGPAGAFCAALVGAEFGKLVSKETKIDIILTPAVTIIAGTLIASAVGPAIGAF
                     MTATGEFIMYATTLRPLLMGMLVSVVVGIVLTLPISSAALCMMLGLSGIAAGAATAGC
                     CAHMVGFAFLSFRDNGWGGVAAQGLGTSMLQVPNLVRNPKCWIPAVIVSAITGPMATV
                     LFRMENSPIGAGMGTSGLVGIIETLNAMGGGMKVWLGILILYIGIPVLLTPLIGNILR
                     KIGWIKDGDLKLDLV"
     gene            6700..7338
                     /locus_tag="PKK75_04720"
     CDS             6700..7338
                     /locus_tag="PKK75_04720"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNZ82932.1"
                     /translation="MAQLYFRYSTMGAGKSIEVQKVAFNYEERGQNPLVFTSSLDNRF
                     AESGVVASRMGLSRRAVAVNEDMDIFEVVREYNDLFGVDAVIIDECQFLKKHHIEELT
                     DVVDELNIPVMCYGLRADFKNELFEGSYWLLALADKIEEIKTICWCGKKALTNARILD
                     GKVVYTGEQIFIGGNESYIPLCRKHYKEGKVMPYKEKGEDLIIRYEKLKKDK"
     gene            7356..7820
                     /locus_tag="PKK75_04725"
     CDS             7356..7820
                     /locus_tag="PKK75_04725"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNZ82933.1"
                     /translation="MKSIFYYDTDIGKISIAENDGFITHVLFGNEKLNDIPVEETPLI
                     KEAHKQIEEYLKGQRSLFDLPLAPSGTEFQKRVWNALKNIPYGKTVSYKDIAIEAGNE
                     KASRAVGMANNKNPISIIIPCHRVIGQNGKLVGYGGGIEIKEYLLRLEKENS"
     gene            7901..8143
                     /locus_tag="PKK75_04730"
     CDS             7901..8143
                     /locus_tag="PKK75_04730"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HNZ82934.1"
                     /translation="MNDIQRKISIRSIIENFISKKHSEKDEMFLNIENAKKEWEDAKN
                     IFENVSQPDLVDYAIYKVEAAEQKYIYLLKQYKSSI"
     gene            8278..9327
                     /locus_tag="PKK75_04735"
     CDS             8278..9327
                     /locus_tag="PKK75_04735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNZ82935.1"
                     /translation="MSTIAAEQIIKKRDKLYELDYMRFIACFAVMIVHITATGIELYI
                     HGSFPYVLVLILNRSIKFTTPVFILLSGATSFYSYCYKKSEFKYMEFLKKRLSKVIVA
                     YFAWCIVYYAAYIYLGYYSLDIKFFIKSLLQGTMSYHLYFVIIIIQMYIAGPIFYFIL
                     KNSERKVTILIIAGIITYLCAEFIRFNLSDRLFLKYMLFYMLGIYITLEYDNYFTWIK
                     KNKVFIVLSYIVMSIIYTLVSYYDSAATTLVWFLISTISLPFVYLTGLWMKDKLKNIY
                     PFIRVFGQSSYYIYLMHPLVLTLMIRYAENNFLSVTDKIILYTFTVIPITVISCLAFT
                     LIRNKFKKRKSAATN"
     gene            complement(9358..9927)
                     /locus_tag="PKK75_04740"
     CDS             complement(9358..9927)
                     /locus_tag="PKK75_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNZ82936.1"
                     /translation="MNMNMTKEKAWELLKEYNKDEFHLQHADTVGFVMEYFAEKLGFE
                     DEKDFWGIVGILHDIDFEMYPEEHCVKMQEILSQNEVDEKIINSAASHGYGLTDVRVK
                     PEHTMEKILFATDELTGLIGAAALMRPSKSTLDMELKSLKKKFKTASFAAGCSREAIK
                     TGAEMLGWDLDYLLDETLKAMQEYEKARQ"
     gene            10215..10979
                     /locus_tag="PKK75_04745"
     CDS             10215..10979
                     /locus_tag="PKK75_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNZ82937.1"
                     /translation="MKDITRVQSIDRAVKILECFSEENKELKLTEISDRLGLNKSTVH
                     GIISTLKYHGFISQDEETQKYKLGIRFVGFGDLVINSMNIRNAAVPVIDKVCEKIEET
                     VHLAMLDGTDVVWIEKRECNKSIKTSTTIGARLPAYTTADGKIIICYQNKDKIKNYLP
                     KRIPQHTKNTITNKVEFISRLFEVKNKGYTIDNEEYVEGLKCVAAPIFDHEGKVRFSL
                     STTGPAFRMNDERIKEIVIIIKEAADEISRRLGYKE"
     gene            11184..12287
                     /locus_tag="PKK75_04750"
     CDS             11184..12287
                     /locus_tag="PKK75_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82938.1"
                     /translation="MKKILAIVLILSISIFAFTACTQDQPKVETPVEEPKEEEPKQEE
                     PKQEEPKTEEPLAEGAVKTGLAVVSSVEKSKDAGEKDGAAQTDSVIVAVTVDKEGKIV
                     QTVIDTAQTKIAFSNEGKVLTDLQTVFKSKQELKEEYGLGKASSIDKEWYEQANALAQ
                     YVAGKTVEEVKAIAVDESGVPTEVDLVSSVTIKINPYKEAIEKAVANAENLGADASDK
                     LGLGVVTTIAKSKDATADKEGLAQAYSTYAVATFDADGKVTSSIIDASQANVNFDASG
                     KITTDLSVAPITKVELGEDYGMKKASAIGKEWYEQADAFAAYVVGKTVEEIKGIAVDE
                     SGHATDEDLVSSVTVGIEDFQSIFEKAYNNSNM"
     gene            12350..13396
                     /locus_tag="PKK75_04755"
     CDS             12350..13396
                     /locus_tag="PKK75_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNZ82939.1"
                     /translation="MIKKAISIILLILIMISLTSCGEKELTKYQSEFILLFDTITRIV
                     GYAETEEEFTGYSQIIHDNLKVYHQLYDIYNDYEGINNIKTINDNAGVKPVKADVKII
                     DLIKFSKELYVKTNGKTNIAYGAVLKIWHEYRTAGIEDPENAVLPSEEELRNASLHTN
                     IEDIIIDEENSTVFLKDPLMSLDVGAVAKGYATEQVSKIVRDSGMYSGLLSVGGNVRA
                     INNNITTNEPWNVGIQNPDNQAEKPVIEVVRLDDRSLVTSGDYERYYIVDGEKYNHII
                     DPETMYPAKYFTSVSIICKDSGVADALSTAVFCIPFEEGIELINLLPETEAMWIFSDK
                     TVKYSDNFESFIKR"
     gene            13553..>15346
                     /locus_tag="PKK75_04760"
     CDS             13553..>15346
                     /locus_tag="PKK75_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82940.1"
                     /translation="MKDSDFIIISKLSKGFIKFFYISAAATIVKVAASFAVPQVIRFT
                     VDHVIDDVPLNYAKPVMNIIDAVGGVEFLKENLWLLAFLVLVLALITGVSDYIGRITV
                     AKGTEGIIKSIRDKLFEHIQKLPYSWHIKNQTGDIIQRSTSDVEVVRNFLSTQLVEMF
                     RTIMLVVISLVLMFSMNFKLTLAVLIFIPIVVLYSTIFYGILAKKFLAADEAEGQLFT
                     TIQENLTGVRVVRAFGREKYEIEKFSQRNITFSDLWVKLGYQLGYYWGLGDLVTGLLL
                     MAIVVYGSYFTVNGVITLGEFLAFASYTGMIAWPLRSFGRILSEFSKTKVSLKRICEI
                     IREPEEFESPDACTPDIYKDIEFKNVTFKYEGDIPVLNNVSFKIKAGTTFAILGGTGS
                     GKTTLMHLLNRLYDLPGDTGSITIGGVDIKNIKREHLRKNIGMVLQEPFLFSRTIKDN
                     IKIAWPEASIDEIKGAASIARVDESIEEFSKGYDTIVGERGVTLSGGQKQRVAIARML
                     LQNTPVMVFDDSLSAVDADTDAKIRSSLKRRKGYSTTILISHRITTLMESDTILVLDK
                     GKVSDIGTHEELINRDGIYKDIFNVQMNLSED"
BASE COUNT         5402 a         2447 c         3095 g         4402 t
ORIGIN      
        1 ccatacttgc cagcgcactt attgaaacag gaagcaggat ggatgattta atattcgaag
       61 aattcaaagg cacaggcaat atggaaattc atttagacag gaagctttca gaaaaaaggg
      121 tatttcctgc cattgatata aataaatccg gcacaagaag agaagagctg ctgcttaata
      181 atgaagaaat gcagacaata tggcaattga gaaaggcaat gagcaacttt tcaactgcag
      241 atgtgactga aagaataatt gacggcttga ttcgtacaaa gacaaatgat gaattcatca
      301 agcaagctgc acaaatttta agcattgaaa aaatgaagtc ggagattaac gacagatact
      361 attaaatgac tgacaaatcg tgataaattc ggcagataaa ttaaaaaaat aaaatattgt
      421 attctataaa agcttatgtt ataatactca aggtaaattg aagataatat atttatatag
      481 aggtagaaag aggtgaacgg tatgaaaaaa gatatacatc ctaattataa aactgttact
      541 gtgaaatgtg catgcggaaa tacattcgaa actgggtctg tattggacga aattagagtt
      601 gaaatatgtt cagaatgcca tccattcttt accggcaaac agaaatttgt tgataagggc
      661 ggaagagttg acagatttaa gaaaaaatac aacatggaat agtatgacct aaccccatat
      721 gttcgagcca tagcgaagaa cagatggctg gcaggtcaac cgcacagaat ccaaatttgt
      781 gcgacgcagg gagcaaacaa atttgatgca atcccctgcg catgacctac aaaaatgagc
      841 ctataacatt tatgggcttt tttattatgc cttttattat ccccaggttc tccatcctta
      901 aggttacaag gcagcttgta gaaggacctc ctgatttttt caaatctatt gaaatgctgc
      961 tttcatattc aaatttaagc ttattgtctt tcaataaatc attaagctca cattcgatac
     1021 ctcttgagcc tatgggaagc atttcattta tatagtcgca aagatacaat ttttcaaagt
     1081 catctatgtc cagtatttta tcagggtttt gcaaaacttc atttccaacc ataggtaggc
     1141 cgactaggag gcagatatcg ccggtttttg tttttttgaa ttttaattta tccttgctgc
     1201 aaaaacctat gcaggtaact cccatggaag tctgcacggt cttgatgttt tcttccgtgc
     1261 tgcctgttaa atgtacgttg aaactcagct ttgacaatac ttcattaaaa ccctcaataa
     1321 gctgcctgcc ggtattgttc atttcaacag ccagtccgtc tgccagaata aagggtgtaa
     1381 atcccatgct cataagctct gcaagaacta cctttcccgt atggtatgca gttaattgag
     1441 gagaagcttt aacaaggtcg tgctccttgt atccaatgcc tccgcaggaa tcgcaggcaa
     1501 tgcccaaata actcttgtgg tcataatcaa ttattgtaat atcccttatc tgccttattt
     1561 ccatttttat ctcctgtcag ggggacattc tttccccctc tttcttattt tatcacatta
     1621 atccgtaaat tccaaattgt taaaattatt taacaatata tatctaaata agttatataa
     1681 ttgaaatgat actattctat tcgttgaggt aaaaatgaaa aaggaactga ctccgctaag
     1741 ggcggatttg atgctggtgg ttgttgcttt tttgtgggga agcagctatg tggtaaccaa
     1801 aggctcaata gacattgtcg gccctgtgca gataatattt ttcaggtttt caatagcgtc
     1861 tgccttaagc ttggtatttt tcggcagata tttaaaaaca tcttcaaaga aagaaataaa
     1921 ggcaggattc atgcttggtg caatgctctg ttttggagca ttatttgcct tttacggcat
     1981 aaggtataca acagtcagca aaaactcatt tataataagc acaaatgtgg tagtggtacc
     2041 atttcttcac tgggcggcct acaagagaaa accaacagga gttagcttgg ctgcagtatt
     2101 tcttatggcc gttggacttg gatttttaac cttggattta agcgggagtt ttaaaataaa
     2161 cataggagat gtaataagct tcggcagtgt tttcttttat gctgcccatg tggtgttatc
     2221 cgataattat tccaaaaggt ataatcctgt ttccgtaaat acatatgcaa tgatatcagt
     2281 atctttttta agctttgcag ttttattgct gacgaataat ttaagaatca acatacccgg
     2341 aaaaattatg ggcgatatgc tgtatctgtc ggttttccct accttcctat gctacactat
     2401 gcagttaaac gcacaaaagc atacaaatgc cacacatgcg gcaataataa tatcattgga
     2461 atcagttttt gcaacagtat tggcagtatt gattctagaa gaaaaaatta ctttacagat
     2521 ggcggctgga tttgtattta tttttatatc ggtgcttact tccgaacttg gagaaacaat
     2581 tttagtaaga tttaaggaaa gacaagcagg cctcttaatc gagataccgg ggaatcaaac
     2641 agaaaacaag gaataatcta gagttgttaa ataaaatatt ctatgctata attaaaaaaa
     2701 tcattaaacc aaggtggtgt atatgtcaca tcaagcaatt tatagaaaat tccgccctaa
     2761 aatatttgat gatgtcctgg gccaggagca tgtaacaagg accttgaaaa atcaaataat
     2821 atctggtaat acagcccatg cttatctttt cagcggaata aggggcaccg gaaaaacatc
     2881 caccgccaaa atatttgcaa gagctgttaa ttgtttgaac aaccatgaag ggaatccatg
     2941 caatgaatgt gaaatatgca aaagcatttt ggatgaaaca aatatggacg ttattgaaat
     3001 ggatgcggca tcaaataacg gtgtagatga cataagggag cttagagaca aggtaaaatt
     3061 tctgcctgtt aaaagcaggt ataaggttta cataatagat gaagtacata tgctgtcaaa
     3121 gggtgcattt aatgctcttt taaaaacctt ggaggaaccc cctgaccacc ttttattcat
     3181 ccttgctact acagagcctc ataaaatacc ggctacgata ttatccaggt gccagagatt
     3241 tgatttaaaa agaataggtt ctgatattat tgttgaaaat atgaaaaaaa tctgcagtga
     3301 cctggatata caatatgaag aaaaagccct gaaattaatt gcagcaaatt cagaaggtgc
     3361 gatgagagat gctcaaagca ttttagacag atgcatatcc ttcaatacga gggttgatta
     3421 tgaaacagtg ataaatttat tgggaacagt aaattatcag gctgtcttga aagcagcaga
     3481 tgcaattgtt gataagaaca tagaaaaaac catgattctg gtggacgata ttttaagcag
     3541 cggtaaagag cttacatttt ttcttgatga attgattatt tgcttcagaa atatgcttat
     3601 aataaaaaca acaaattctt ctgacaatct aatgaggata tctgaggatg aagcaaatga
     3661 aattgtagaa ttaaacaaaa aaatcactgt tgatgaaaca gttaaaataa tagaggacct
     3721 gtcaacagca caagcagaat gcaaaagggc gataaatcca agaattcttt tagaaacaaa
     3781 gcttattaaa atgctgcata acgtaaatta tgtaagtaaa gatgacttag tcaaaagaat
     3841 tgagagcata gagaacagcc tgcatgaagg atttaagaca aaagacgcag aaaggatacg
     3901 ggaaaaccgg actgcaagga taaataaaga gccggatatc aaagaaaaca tcggaggaac
     3961 aaaaaagaca gcaggcaaag aaaagcctga aataccgcca aaagaaaatg aagaagccct
     4021 tgacagcaaa gaggacaaaa gcgacgaagc agtttttaat accatcttaa gccagtggca
     4081 ggctatactt aaagaaatca gaagcgaaaa tgcaggcctg catgctattg tcagagaatc
     4141 caggctgatc gaggtaaaaa ataaaaaagc agtatttgaa tttgacccta agtttacatt
     4201 ccacatcagt gctgcaaatc agcctaaaaa caaggatcag tttaaagctg ccttgagacg
     4261 atttttgaat gaagattgtg aagtcgagtt tatcactaaa aaggaagaaa aacatataaa
     4321 gcaaaaaaca actatcaaag atgtatatga agatttgaaa aatgagtttg gagaaatagt
     4381 agaatacaaa caggaggatt aaaaatggcg aaaggcagaa aaatgcccat gggtatgccc
     4441 ggcggcggaa atatgaacaa tatgatgaag caggttcaaa aaatgcagaa acaaatggag
     4501 gatatgcaaa aagaacttca acagagagaa gtggaagcaa catcaggcgg aggagcagtt
     4561 acagtcaagg ttgacggtaa aaaaaccttg ctggagctta aaatagatcc tgaagtagta
     4621 gacaaagacg atgttgaaat gcttgaagac atgattgttg ccgccattaa cgaagctttc
     4681 agaaaagctg atgaaatgac ggacagtgaa atgaaaaaaa taacaggcgg agtaaacata
     4741 ccggggttaa tgtgatggag cattacaccg cacctgttac gaaactaata gaagaaatgg
     4801 caaagcttcc gggaatagga agaaaatcag ctcagcggct tgcttttcat attcttgata
     4861 tgaaagaggc tgatgttgtt gagcttgcaa aagcaatagt taatgcaaaa aaatatacaa
     4921 aacactgtca aagatgcggg aacatgacag aaggcgagct atgcagcatt tgcagctctt
     4981 ttaagagaga tatgtcaacc atatgtgttg ttgaagatgt aagagatatt gcagccatgg
     5041 agcggaccaa ggagtacaaa ggtgtctacc acgtccttca tggaacaatt tctcctctcg
     5101 atggaattgg tcctgatgat ataaatatca aaagtttaat aacaaggctt aatgcagatg
     5161 taaaagaagt aattttagcg acaaatccaa ctattgaagg agaggcaacg gcagtttata
     5221 tatcccgtct cataaagcca atggggatta aagtaacccg catagcccat ggaattccag
     5281 taggcggaga catagaatac gcagacgaag ttactctcat gagagccatg gaaggcagaa
     5341 gagaaatgta gaaagcatga atttcatgct ttttcaactt ttaacaaagg atcagaccca
     5401 caaacttttc taactgcagg ttagccagca aaataaagtt cagggaaaaa aattgaattt
     5461 atggagaaag cggcttagaa tataagttat aagaatacaa gttatagagg gataaaaatg
     5521 agtaaaaaaa atgagaatga aaaggaatca aaatttaaaa gctttttaag aaaaaagaat
     5581 attaagattt cagttcaaag atacctgatt gaagccttag gactcatggc ttacggctta
     5641 ttcgcgtcct tgctgatagg gactatcctc aataccatcg gcaaaaaggc cggcatttta
     5701 tttttaactg aaacaatata tccaatttgc ctacagatga caggggcagc aatcggcgtt
     5761 gctgttgcct atgggctgaa agctcccccc ttagtcttgt ttgcttctac aattacggga
     5821 accgcaggca atgtcctggg aggaccggca ggagcctttt gcgcagccct ggtaggggct
     5881 gaatttggca aactggtttc aaaggaaaca aaaattgata ttattcttac acccgctgtt
     5941 acaattattg caggtacttt gattgcttct gctgtaggac ctgcaatagg agcttttatg
     6001 actgctacgg gtgaatttat tatgtatgct acaaccttga ggcctctttt aatgggcatg
     6061 ctggtttcag tggtggtagg catagtattg acactgccca taagcagtgc agccctatgc
     6121 atgatgcttg gtctttcagg cattgctgca ggtgcagcaa ccgccggatg ctgcgcccat
     6181 atggttggat ttgcgttttt aagcttcagg gataacggtt ggggaggagt tgctgcccaa
     6241 ggactgggaa catcaatgct gcaggtgcca aatctggtga gaaacccgaa gtgctggata
     6301 cctgctgtaa tagtatctgc aattacgggt ccgatggcaa cagttttatt ccggatggaa
     6361 aacagcccca taggggcagg catgggaacc agcgggctgg taggaatcat tgaaacatta
     6421 aatgccatgg gcggcggaat gaaagtgtgg ctcggaatat taatattata cataggaatt
     6481 cctgtgctgc ttacccctct tataggtaat attttaagaa aaataggctg gattaaagac
     6541 ggagacttga aactggattt agtataacag gaatatgcgg tcggtattta cataattaca
     6601 tttatgtaaa ccaagggaaa atttaaagaa tacataattt cttttatgta aacaattgaa
     6661 aataattaaa atttataaca cattatgaag gagacaatta tggcgcagct ttattttaga
     6721 tacagcacca tgggtgccgg aaagagcata gaagttcaaa aggttgcatt taattatgaa
     6781 gaaagaggac aaaaccccct ggtatttaca tcttcactgg ataacaggtt tgcagaatcg
     6841 ggagttgttg cctccaggat gggacttagc aggagagcag tagctgttaa tgaggatatg
     6901 gatattttcg aagttgtaag ggaatataat gatttattcg gtgttgatgc agttataatt
     6961 gatgaatgcc agtttttaaa aaaacaccat atagaagagc ttacggacgt agttgatgag
     7021 ctgaatattc cggttatgtg ctacggactt agagccgatt tcaaaaatga attattcgaa
     7081 ggttcatact ggcttttggc attggcagac aaaatagagg aaattaaaac catttgctgg
     7141 tgcggcaaaa aagctttaac caatgcccgt attttagacg gcaaagttgt atatacaggc
     7201 gaacaaattt tcataggtgg aaacgaaagt tacattcctc tctgcaggaa gcactataag
     7261 gaagggaagg tcatgcctta caaggaaaaa ggcgaagacc tgataataag atatgaaaag
     7321 ttaaaaaagg ataagtaata attggcggag gaagaatgaa aagtatattt tattatgata
     7381 cagatatagg taaaataagc attgcagaga atgacggttt cattacacat gtcttgtttg
     7441 gaaatgaaaa attaaatgat attcctgtag aagagactcc acttatcaag gaagcacata
     7501 aacagataga ggaatatcta aagggtcaaa gaagcttatt tgatttgccc ctggctccat
     7561 ccggtacaga atttcagaaa agagtatgga atgctctaaa gaatattcct tacggcaaga
     7621 ctgtaagcta taaggatatt gctatagaag ccgggaatga aaaagcatca agggctgtag
     7681 gtatggctaa caacaaaaat ccaatttcaa tcatcatccc ctgccacagg gtaattggcc
     7741 aaaacggcaa gcttgtagga tacgggggag gcatagaaat aaaagaatat cttctaaggc
     7801 tggaaaaaga aaactcatag aacgacatat attaattttg gaatattagg aataaaaagg
     7861 aaccttctgt ctataataat aaagatagga ggttttttcc atgaacgata ttcaaagaaa
     7921 gattagtata agaagcataa tagagaattt tatatcaaag aagcattcgg aaaaggacga
     7981 aatgttttta aatattgaaa atgctaaaaa agaatgggaa gatgcaaaaa atatttttga
     8041 aaatgtgtca cagcctgact tggttgacta tgcaatttat aaggtggaag ctgcggaaca
     8101 aaagtatata tatcttttga agcaatataa gagcagtata taaattgaaa taataatata
     8161 aatctaatgt aagacggcgg attatcccaa catttttaat ttatggattt tcaaaaggtt
     8221 tgcatttgtc cctcataaat gataaaataa ccaaaatatt cttaaaagag tgatgtaatg
     8281 tcaacaattg cagcagaaca aattatcaaa aaaagagaca agctttatga actggactat
     8341 atgagattca tagcatgttt tgctgttatg atagttcata taacagcaac aggaattgaa
     8401 ctctatattc acggtagctt tccatatgtt ctggtgctta ttttgaacag aagcattaaa
     8461 tttactactc cggtttttat tcttttaagc ggggcaacaa gcttttacag ctactgttac
     8521 aaaaaaagtg aattcaagta tatggaattt ctcaaaaaaa gactttctaa ggttatcgtt
     8581 gcttattttg cctggtgtat tgtttattat gccgcttata tatatttggg atattacagt
     8641 cttgatatta agttttttat aaaaagcctt ttacagggaa caatgtcata tcatctttac
     8701 tttgtgataa ttattattca gatgtacatt gcaggcccca tattctattt tattttgaaa
     8761 aattccgaaa ggaaagtgac aatactgata attgcaggaa taataactta tttgtgtgct
     8821 gaattcataa ggtttaattt atcagacaga ttattcttga aatacatgtt attttatatg
     8881 ctgggcattt atattacttt ggaatacgat aattatttta catggatcaa gaaaaataaa
     8941 gtgtttatag ttttgtctta tatagttatg agtataattt acactcttgt ttcatattat
     9001 gattctgctg caactactct tgtatggttt ctgatttcaa ccatatcatt gccttttgta
     9061 tatttaacag gcttatggat gaaggataaa ttaaaaaata tttacccctt tataagagtt
     9121 ttcgggcaaa gctcctacta tatttatttg atgcatccgc tggttttgac tcttatgatt
     9181 agatatgcag aaaacaactt cttatcggtt accgataaaa taattcttta cacatttaca
     9241 gtaataccga ttacggtaat atcctgcttg gcattcactc ttataagaaa taaatttaag
     9301 aaaagaaaat cagcagcaac aaattaaaaa ggacatgcgt ggcatatcct ttatttttca
     9361 ttgtcttgct ttttcgtatt cctgcattgc ttttaatgtt tcatccagca aatagtccaa
     9421 atcccatccc agcatttctg ctccggtttt tatggcttct cttgagcagc ctgcagcaaa
     9481 acttgctgtc ttgaattttt tctttaaaga cttaagctcc atatccaagg tactctttga
     9541 aggacgcatc aatgctgcag cacctatcaa acctgtcagt tcatcagttg caaaaagtat
     9601 tttttccata gtatgctcag gttttactct tacgtctgta agcccgtatc cgtggcttgc
     9661 agccgagttg ataatttttt cgtctacttc gttttgggac agtatttcct gcatttttac
     9721 gcaatgttct tcaggataca tttcaaagtc aatatcatgc aaaattccta caatccccca
     9781 aaaatccttc tcatcttcaa agcctaattt ttctgcaaag tattccatca caaagcccac
     9841 cgtatctgca tgctgcagat gaaactcatc tttattgtac tccttcagca attcccatgc
     9901 cttttcttta gtcatattca tattcatatt catatatccc ttccttattg taataacata
     9961 attttaactt atttgggttg acatgtctat aataaaattt ctaaaacatt ttcaacttta
    10021 catatagatt gacaagcaat ttacacagtg ataatattat cataatatag aattcgacat
    10081 gataaaactt aattctaata gcaaataatt ggaaaatgta atatgtttgg aatcacatac
    10141 gcaatcgatt gttaataaat tatttatatt gatttttagc agtgctgtat gttataattt
    10201 tggagaattt aattatgaaa gatataacca gagttcaatc aattgacagg gcagtaaaaa
    10261 tactggaatg tttcagtgaa gaaaacaaag agcttaagct gacggaaatt tctgatagat
    10321 taggcttaaa caagtctact gttcatggta ttatatctac cctcaagtat cacggtttca
    10381 tctcccagga tgaagaaact caaaaataca aactgggcat acgatttgtc ggattcggtg
    10441 acttggtaat taattcaatg aatattcgaa acgcagcggt gcctgttata gataaggtct
    10501 gtgaaaaaat tgaagaaact gttcatttag caatgcttga cggaacagat gtagtatgga
    10561 tagaaaaaag agaatgcaac aagtcaataa aaacttccac tacaatagga gccaggctac
    10621 cggcatatac aactgcagac ggtaaaataa tcatatgtta tcagaataag gataagatta
    10681 aaaattattt accgaaaaga attccacagc atacaaaaaa tacaataaca aataaagttg
    10741 agttcatcag tagattgttt gaagttaaaa ataaggggta cactatagac aatgaagaat
    10801 atgttgaagg attaaaatgt gtagctgcac caatatttga tcatgagggg aaagtaaggt
    10861 tcagtttgag cacaacagga cctgccttca ggatgaatga cgaaagaata aaagaaattg
    10921 tcattataat taaagaagct gcagatgaaa tatcgagaag attaggatat aaagaataat
    10981 tttataggat ttcgggggac caaaatccta ttaaaaatag tataatccag aacatagttc
    11041 agcatagctg aacaaaagct ataaaatttt caaaaaatgt gttgactaat acgaaacaat
    11101 ttgttaaaat cgtaccgaat tgcacaacaa agttataaac agctttaagc tgattatata
    11161 tatttaaaat atggaggaaa gtaatgaaaa aaatattagc aatagtgttg atcttaagca
    11221 tatctatatt tgctttcaca gcatgcacac aggaccaacc aaaagttgaa acaccggtgg
    11281 aggaacccaa ggaagaagaa ccaaaacaag aagaaccaaa acaagaagaa cctaaaactg
    11341 aagaaccatt agctgaaggt gctgttaaaa caggattggc agttgtatca tctgtagaaa
    11401 aatcaaagga tgcaggcgaa aaagacggag cagcacaaac agattcagtt atagtagcag
    11461 taactgttga caaagaaggg aaaatagtgc aaactgtaat agatacagct caaactaaaa
    11521 tcgcttttag caatgaaggt aaagtattaa ccgatttaca aactgtattt aagtctaaac
    11581 aagagctaaa agaagaatat ggactgggta aagcttcaag tatcgataaa gaatggtatg
    11641 aacaagctaa tgctttagcc cagtatgtag caggcaaaac agttgaagaa gttaaagcta
    11701 ttgctgtgga cgaatcagga gttccaactg aagttgattt agtttcatca gtaactatca
    11761 aaattaaccc atataaggaa gctattgaaa aagctgtagc aaatgcagaa aatttaggag
    11821 ctgatgcttc cgataaatta ggattgggag ttgtaactac aattgccaaa tcaaaagacg
    11881 caacagccga caaagaagga ttggctcagg cttattcaac atatgcagta gcaacatttg
    11941 atgcagacgg aaaggtaact agttcaataa tagatgcttc tcaagctaac gtgaattttg
    12001 atgcatcagg aaaaatcacg actgatttat ctgttgcgcc tataactaaa gttgaattag
    12061 gcgaagatta cggaatgaaa aaagcttcag ccataggcaa agaatggtat gaacaggcgg
    12121 atgcatttgc agcatatgtt gttggcaaga ctgttgaaga aataaaaggt atagcagttg
    12181 atgaaagcgg tcatgctact gatgaagact tggtatcatc tgttacggtt ggaattgaag
    12241 acttccaaag cattttcgaa aaagcatata acaattctaa tatgtaacaa ttctaacatt
    12301 taatcatcac ggggcagatt ctgccccgtg tttttttaag aggtgcaatt tgataaagaa
    12361 agcaatttca ataatattgt taatattaat tatgataagt ttaacctcat gcggcgaaaa
    12421 agaacttacc aagtatcaat ctgaatttat tttacttttt gatactatta cacgaattgt
    12481 gggatatgca gagacagaag aagagttcac cgggtattct cagattattc atgacaattt
    12541 gaaggtatat catcagcttt atgatatcta taatgattat gagggtatta acaatattaa
    12601 aactatcaat gataatgcag gagtaaagcc tgtcaaggca gatgtaaaaa taattgactt
    12661 gattaagttt tcaaaagagc tttatgtgaa aacaaacggc aaaacaaata ttgcctacgg
    12721 ggcagttttg aaaatctggc atgaatacag gacagcagga atagaggacc ctgaaaatgc
    12781 agttcttcct tcagaagaag agttaagaaa tgcctctttg catacaaata ttgaagatat
    12841 aatcattgat gaggagaatt caacagtatt tttaaaagat cctcttatga gtcttgatgt
    12901 tggagccgta gcaaagggat atgcaaccga gcaggttagc aaaatagtaa gggacagcgg
    12961 aatgtattca ggattattgt ccgtaggagg caatgtacgt gctataaaca ataacatcac
    13021 cacaaatgaa ccatggaatg taggaattca aaatccggat aaccaggctg aaaagccggt
    13081 aatcgaagta gtcaggctgg atgacaggtc tttggtaaca agcggtgatt atgaaagata
    13141 ctacattgtt gacggagaga aatataatca tataatagat cctgaaacga tgtatcctgc
    13201 aaaatatttc acttcagtat ccataatctg caaggattca ggtgttgccg atgccttatc
    13261 aacagctgtc ttctgcatac cttttgagga gggtattgaa ttaattaatt tattgcctga
    13321 aacagaagca atgtggattt tcagtgataa aacagttaaa tacagcgata atttcgaaag
    13381 ctttattaaa agatagcacc tggtttttat tgacactaat tgaatattaa tatagaatta
    13441 ataagcaatc ctatagggat tgcttatttg gcatgtcata accccatttt tcagaatatt
    13501 ttttccctgc atgcctaagc ccatatagta agcacccatt aagaggtttt gaatgaaaga
    13561 ttcggatttt ataattatca gtaaattgtc aaaaggattt ataaagtttt tctatatatc
    13621 tgctgcagca accatagtaa aggttgctgc aagttttgct gtgcctcaag ttatcaggtt
    13681 caccgtagac catgtaattg atgatgttcc cctgaattat gcaaaacctg ttatgaatat
    13741 aattgatgct gtaggagggg tagaattttt aaaagaaaac ttatggcttc ttgccttctt
    13801 agtattagtt ttggctctta tcacaggagt gagcgattat ataggcagaa ttaccgttgc
    13861 taagggaaca gaagggatta taaagagtat tagggataaa ctgtttgagc acatacaaaa
    13921 gttaccctac agctggcata taaaaaatca aaccggagat ataatacaaa ggtcgacatc
    13981 cgatgtggaa gtggtccgta attttttaag tacgcaattg gttgaaatgt tcagaacaat
    14041 catgctggtt gtaatatcct tggtattaat gttttcaatg aactttaagc ttactcttgc
    14101 agtattgata tttatcccta tagtggtttt atattcaaca attttctatg gaatactggc
    14161 aaaaaaattt ctggcagccg atgaagcgga aggacagctt tttactacaa ttcaggaaaa
    14221 cctgacaggg gtccgtgtgg taagggcctt tggccgggaa aaatacgaaa tagaaaagtt
    14281 ttctcaaagg aatattacct tttctgacct ttgggttaaa cttggatatc agctgggcta
    14341 ttattggggg ctgggtgatt tggtaacagg gttactcctt atggccattg ttgtctatgg
    14401 ctcttatttt accgtaaatg gtgtaataac attgggcgaa ttcttagcat ttgcttctta
    14461 taccggaatg attgcatggc cccttcgaag ctttggaaga attttatctg agtttagcaa
    14521 aacaaaggtt tctctgaaaa gaatctgtga aataataaga gaacctgagg aatttgaaag
    14581 ccctgacgca tgtactccgg atatttataa ggatattgaa tttaaaaatg ttacatttaa
    14641 atatgaaggg gatattcctg ttttaaacaa tgtaagcttt aaaataaagg cagggaccac
    14701 atttgccata ttaggcggta ccggttcggg aaaaactact cttatgcatc tcctaaaccg
    14761 cttgtacgac ctgcccggcg atacgggcag cattaccata ggcggagtgg acattaaaaa
    14821 tattaaaaga gaacatttaa gaaaaaacat tggaatggta cttcaagaac ccttcttatt
    14881 ttcacgtaca attaaggata acataaaaat agcatggcct gaagcttcta ttgatgaaat
    14941 caagggagct gcctcaatag cacgggttga cgaatcaata gaagaatttt caaagggcta
    15001 tgatacaatt gtgggggaaa gaggagtaac attgtcggga gggcaaaagc aaagagttgc
    15061 aatagcaaga atgctgcttc aaaacacacc ggttatggta tttgatgatt cactgtctgc
    15121 tgttgatgca gatacagatg caaaaattcg ctcctccctc aaaagaagaa agggctattc
    15181 aacaactata ttaatatcac accgcataac aactttaatg gaatctgata ctattttagt
    15241 attggacaag gggaaagtat cagatattgg aacccacgaa gaactgataa acagggatgg
    15301 catttataag gacatattca atgtccagat gaacctgtcg gaggac
//
LOCUS       DAOPUT010000037        15304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_3937_length_15304_cov_19.263624, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000037 DAOPUT010000000
VERSION     DAOPUT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15304
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_15304_cov_19.263624"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..646)
                     /locus_tag="PKK75_04765"
     CDS             complement(167..646)
                     /locus_tag="PKK75_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82941.1"
                     /translation="MKVTIISESGDWKEMLYTKDIVSYDVKNQEILDFFIQLGDAYSN
                     NDTLDDMFECLYNLINYGYLYTDDNIDDNYYDNFDYDDDYFDFDDEDQDFISQDGFKL
                     IKAEVEYYVDDELVSKDEYDDAWDDRIGYLGSSNWEEKGLTIINSETDEVIFLGKMS"
     gene            complement(875..1600)
                     /locus_tag="PKK75_04770"
     CDS             complement(875..1600)
                     /locus_tag="PKK75_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ82942.1"
                     /translation="MKIRYIYHSCFLIETTNAFLLFDFFKHKSDKGGFNFEKLLSDVF
                     ASPKALYIFSSHSHQDHFTYDVFSWINKKPNTYYILSSDIKIYNRVENSYVVRPGDQL
                     SVNEIKLDIFGSTDEGVSFLVNIEGLNLFHAGDLNWWKWYDDTPEEEKAMEDSFKSIV
                     ADISKFGVKIDVAFFPVDSRLELNYLCGGQYFIEQLKPKLFFPMHMWDNYETAQDFKK
                     AVAGYSTNIIEIKHPNEILYANI"
     gene            1852..3504
                     /locus_tag="PKK75_04775"
     CDS             1852..3504
                     /locus_tag="PKK75_04775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ82943.1"
                     /translation="MKIENVVLNDSIQRALDDMGFEESTQIQQETIPLIMEGKDIVGQ
                     SNTGTGKTAAYAIPILEKIDKELKMPQALVLLPTRELAVQVANEVRKIGKYMDGIKTV
                     TVYGGADIKDQINKLKAGAQIIAGTPGRIIDLIERHVIKLGEIKITVLDEADEMLKMG
                     FREDIELILGKIDHPVQSLLFSATIPDDIKKIINKFLHSPVSVKVLREGITAKEVKQS
                     YFLVKHSDKVEALARLIDTYTPKLTLVFCNTKRGVDELYDQLVDKGYNCDKIHGDIKQ
                     SQRLDTLNKFNNGLIEILIATDVAARGLDIKEVELVINYEVPSKEDYYVHRIGRTGRA
                     GKEGASFTLASAKELRKIEDIEKYTKKSIRKRTIPTVDKVNEVKQDKFIRGIVSVIES
                     GDLGENRELAGKLMDKGYDAETVIAAMIKKLVKFDLSEERDLNDVLPERRKRGEVKLE
                     NTERFHVNLGKKQGIRPGDILGAVAGECDIPGYDIGEIEVLDNFSFFTASKDHKHRIL
                     DRMNRSQIKGMDVAVELSKEKKSRFAREKSSDDKSFWKRKKR"
     gene            3872..5797
                     /gene="thrS"
                     /locus_tag="PKK75_04780"
     CDS             3872..5797
                     /gene="thrS"
                     /locus_tag="PKK75_04780"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNZ82944.1"
                     /translation="MIKVTFPDKSVREYEKGIKAVDVAKSISDGLARNVVGAVYNRKS
                     IGLQNTLNEDGSIKFLKFEDKEGKDIFWHTSSHIMAHAIKRLWPDAKLAIGPAIENGF
                     YYDIDLEHRFIQEDFEKIEAEMKNIVSAGYELERFELPREEAINFMKEAKEDYKVELI
                     QDLPADAVISFYKHGDFIDLCAGPHLESTKKPKAFKLLSIAGAYWRGNENNKMLQRIY
                     GISFEKAKDLEDYLKMMEEAKRRDHRKLGKELDLFMMSDEGPGFPFFLPKGVVVKNEL
                     MKYWREMHKKAGYVEIETPLILNRHLWEVSGHWFTYRENMYTTVIDEQDFAIKPMNCP
                     GGILVYKNAMHSYKDFPMRVAEAGRVHRHELSGALHGLMRVRAFTQDDAHLFILPEQI
                     RDEIKGVIKLIDEIYKQFGFSYKLELSTRPEKFLGEIKDWDLAEASLKAALDELNLAY
                     VINEGDGAFYGPKIDFHLTDCIGRTWQCGTIQLDYQLPQRFEMEYVGADGQKHRPIMI
                     HRTAFGSIERFFGILIEQYAGAFPTWLAPVQVKILPISDKFSDYAKEVENKLNALNIR
                     VETDSRAEKIGYKIREAQVERIPYMFVVGEKEAESNTVSVRERHQGDIGAMSIDEISS
                     IILDKIKNRENDFPQLL"
     gene            6035..6805
                     /gene="udp"
                     /locus_tag="PKK75_04785"
     CDS             6035..6805
                     /gene="udp"
                     /locus_tag="PKK75_04785"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HNZ82945.1"
                     /translation="MGTVYSDAQYHIDMKEGNVGRYVILPGDPKRCSKIAKYFDNPVL
                     VADNREYVTYTGYLDGVKVSVTSTGIGGPSAAIAMEELANIGADTFIRVGTCGGMSVD
                     VMGGDLVIATGAIRAEGTSREYAPVEFPALADFKIANALVKAADNLHKKHHVGVVHCK
                     DSFYGQHEPESKPSSYELMNKWEAWLRMGTLASEMESAALFTVAAYRKVRAGSVFLVV
                     ANQERRRLGLEDPQEHDTEAAIATAVEALRILIREDNK"
     gene            complement(6850..8295)
                     /locus_tag="PKK75_04790"
     CDS             complement(6850..8295)
                     /locus_tag="PKK75_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ82946.1"
                     /translation="METVKKFKSYSLALDLVEDGRDIQIEDIKKHQLPPKVTSKSLYS
                     DIVKIAWPSVVELTLTQLASMVDLMMVGGLGAWALTSVGLTVQPKFLLMTFFMAMNVG
                     ATAMAARYKGAGEQEKANLILRQALVLTLIFSAVSSVIGYVYAEKFILFMGAAEEKTL
                     QGGTQYFKIQMIGFPFMALTSTITATLRGVGNSRTAMIYNLASNVVNVILNYLLIEGN
                     LGFPRLEVAGASLATIIGQFLAFAFALIYIMKGDQYLHLRFNDGFKPDPPTLKQIVAI
                     GAPAMLEQLLMRAGVIIYVRTIAGLGTVAYAVHQVCMNIQAMSFMNGQGFAVSATSLT
                     GQSLGKNRPDMAHAYVHRTRRLGMSVSILLGIIFIFWGGKIVSLYNDDPAIVSQGARI
                     MMFVGFTQPLQSSQFILAGSLRGAGDTRATAIITFITVLLIRPFLAMYLINAWNMGLD
                     GAWFAFIVDQTIRSALVLIRYNSGKWKTINV"
     gene            8669..9796
                     /locus_tag="PKK75_04795"
     CDS             8669..9796
                     /locus_tag="PKK75_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNZ82947.1"
                     /translation="MSMLLSIFLIGFFGYMLGGIKIYSIELGTAGVLLVALVFGHYGI
                     VTPDIVREMGLVCFVTSVGFVAGPNFFRNFKQNAASYVLIGVLMIISSALSCVLIVKV
                     SGISPALAVGLLTGALTSTPGLAAAIEAAGEYGNLASIGYGIAYPFGVISVVLFVQLI
                     PKILKADMAKEREVLEAVTDITKSKSIFQNLYSFDNAGFFSVCMAVVLGIFLGRASIP
                     LPGGAKFSLGNSGGPLIVGLIFGHFGHIGQFNISVKKPVLETLRELGLMLFLIGAGTN
                     AGQGFVEILKQYGLMLFYYGAIMAIIPMIIGFYLSGKILQVGLLNTLGAITGGMTSTP
                     ALGALIEAAKTDDVASSYAATYPVALVSVVLASQFIIILFG"
     gene            9922..10449
                     /locus_tag="PKK75_04800"
     CDS             9922..10449
                     /locus_tag="PKK75_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82948.1"
                     /translation="MNKTKIKIKYIGGNMKVTLEQIDELRNRVNVSYEEAKRTLEKND
                     GDLIKSIIELENKKGRKPEQRGSFTSFADRLLALKMSVKNKEGNTVLNVPVVVVLITF
                     LMSFWTVIFLFVLAVLTSCKIDVYRDKSTNVNSIKKNMQDTVDKIKVKSDEIFKDEKK
                     PEDTKDDSENEIIIE"
     gene            10576..11256
                     /locus_tag="PKK75_04805"
     CDS             10576..11256
                     /locus_tag="PKK75_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ82949.1"
                     /translation="MAKKKILIVEDEQKIARFLELELKYEGYDVTIANNGREGFEQGK
                     SPDIDLIILDLMLPGLSGIEVCRRLRQTSDVPIIMLTAKDDISDKVTGLDIGADDYMT
                     KPFAVEELLARIRVLLKRKYHKNDDEDLLIVGKLKLSKNNYKVEYDNDNIELTKKEFE
                     LLEYLMRNKNIVLSREKILDNVWGYDYYGDTNIIDVYIRYIRSKIDQKYDLNLIETVR
                     GVGYIIRD"
     gene            11267..12679
                     /locus_tag="PKK75_04810"
     CDS             11267..12679
                     /locus_tag="PKK75_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ82950.1"
                     /translation="MVKTIIGKLLNIILYPFRKIVEALVSLIKRLRFSIAFKISTTYL
                     ILYIIIIMTVALTTSVGYLIFELQKFEESTAAEDSAIIKSSFPDFNNIDVSGKTYNSF
                     EIFDSSLNTYYSSGKPGRYADSLIVIAEKIIIDNEYVHSESFMKNGSMYYMNVNYPME
                     DMLRDAWKISFMVLISGAIGLMFFAPIVSSTSYKLIGPIKNMTEITRTITVNNINTRL
                     DVKGTEDELKELSETFNEMMDRIERGYKTQQQFVSDASHELRTPIAVIKGYVNMLDRW
                     GKNDKAVLDESIEAIKNETDSMQNLIEKLLFIARSDRSTLNFTKEDFRISTVLNEIEK
                     ETRMIDTTHKLYFKFYDDATIYGDKMRIKQAVRILLDNAMKYTPPGKYIMVSGYIEDE
                     YYVIKVEDTGEGIQKNDLDKIFDRLYRAEQSRSKKISGHGLGLSIAKIIVLGHKGKIK
                     VKSTPGKGSEFSLLFPHLDN"
     gene            12784..13089
                     /locus_tag="PKK75_04815"
     CDS             12784..13089
                     /locus_tag="PKK75_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HNZ82951.1"
                     /translation="MNIQFIETTNVLTIKLKGELDHHSADEARVLIDEKIKNENFNKL
                     VIDLKGLDFIDSSGIGFVIGRYKVIRKRNGVIEIVNAGKKIRKILDMSGIGKIIKIK"
     gene            13099..13578
                     /gene="spoIIAB"
                     /locus_tag="PKK75_04820"
     CDS             13099..13578
                     /gene="spoIIAB"
                     /locus_tag="PKK75_04820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HNZ82952.1"
                     /translation="MHNNYVYIKIPSLSVNESFARAAVAAFCSSLNPTMNEISDIKTA
                     VSEAVTNAVVHAYEDEIGEIEIQCRIKGQVVEIIVEDFGCGIADVEKAREPLFTTRPE
                     LERSGMGFSVMESFMDELVVLSEKDVGTKVILRKKISEKKLKTLNEESDDESSEDEE"
     gene            13873..14313
                     /gene="spoVAC"
                     /locus_tag="PKK75_04825"
     CDS             13873..14313
                     /gene="spoVAC"
                     /locus_tag="PKK75_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HNZ82953.1"
                     /translation="MKYNKNNYKKVVDEFTPKNKILKNCLMAFIFGGTICLIGEIIND
                     SFVGWLGISDDEASPMTSVVLVGLSAVLTGLGWYDKLGKIGGAGMVVPITGFANSVVS
                     PALEYKREGFILGTAANIFKLAGPVLLFGYFSSFIVGLLSMLFK"
     gene            14323..>15304
                     /gene="spoVAD"
                     /locus_tag="PKK75_04830"
     CDS             14323..>15304
                     /gene="spoVAD"
                     /locus_tag="PKK75_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HNZ82954.1"
                     /translation="MKKTGKTFIIKDDVIIRDTYTIAGSKEGQGPLKGRFDMVLDDDL
                     WEEDSWEKCELKMQKTAVENLKNKNSLINENIDLLISGDLLNQITSSSFAARELELPY
                     IGVYGACSTMTLTMGLGALIIDGGFAENVICVTSSHFCSAERQYRLPLEMGGQRHMSA
                     QWTVTGSAAVWLSNSGKGPKIKHVTFGKIVDLGVKDANNMGAAMAPAAYDSLSRHLQD
                     TGIDYDLIVTGDLGKVGKSIVNKMFDEDNKSIESKYDDCGTIIFDVNKQDVHAGGSGC
                     GCSATVFGSFLYKKLLNNEIKKLLFTSTGALHSPTATLQGESIPGIAHCLG"
BASE COUNT         5278 a         2477 c         3208 g         4341 t
ORIGIN      
        1 atggtggaag tgccccagga agcattcctg gcggtgctta aatttgatga agattgagga
       61 aataggtatg gggacacacc ataagaggta tggggacaca ccttcaaaat taagtaactc
      121 tctgcgtgat agcttttgaa ggaatgtccc cgattttacc cctgttttat gacatcttac
      181 ctaaaaaaat cacttcatcg gtttcggaat taataatggt taaacccttt tcttcccagt
      241 ttgatgagcc cagatacccg attctgtcat cccatgcatc gtcgtattca tcctttgaca
      301 ccaattcatc atcaacatag tactcaactt ctgctttaat taatttgaag ccgtcttgag
      361 aaataaaatc ctggtcttca tcatcaaaat caaaatagtc atcatcataa tcaaaattat
      421 cgtaataatt atcatctata ttatcatcag tataaagata gccgtaattg attaaattgt
      481 acaaacattc aaacatatcg tcaagcgtat cattatttga gtaggcatcg cctaattgta
      541 tgaaaaagtc caatatttcc tgatttttaa catcgtatga aactatgtcc ttggtataga
      601 gcatttcttt ccaatctccg ctttcactga ttatagttac cttcactaaa actcccccct
      661 tgatacaaaa actgttagta aaatattatg cattgaaaaa catattatgt attatgatta
      721 caaaaatggg agaaaccaaa atctcataaa aaaagtgacc atattaaaca ggtcatcttt
      781 tttattaatt aattcattag cccatcccag gttgttaatt ccttatctga tgtaattata
      841 actaaacagc gaatttatca gtaaaattat cttactatat atttgcatac aatatttcat
      901 tagggtgttt gatttcaatt atattcgtgc tgtatccagc cactgccttc ttaaaatcct
      961 gagcagtttc ataattatcc cacatatgca tggggaaaaa aagctttggc tttaactgct
     1021 caataaaata ctgacccccg cataaataat ttaattccag cctgctgtcc acagggaaga
     1081 aagcaacatc aattttcaca ccaaactttg aaatgtccgc tacaatactt ttaaaagaat
     1141 cttccatggc tttttcctct tcaggtgtat catcatacca tttccaccag tttaaatcgc
     1201 cggcatgaaa taaattaaga ccctctatat tgaccaaaaa gcttacccct tcatcggtag
     1261 acccgaaaat atcaagcttt atttcgttga ctgaaagctg gtccccgggt cttacaacat
     1321 atgaattttc aactctgtta tatattttaa tatcagatga caatatgtaa taggtgtttg
     1381 gctttttatt tatccaggaa aaaacatcat aagtgaaatg gtcctggtgt gagtgagagc
     1441 taaatatata gagagcttta gggcttgcaa atacatcact aagcaatttt tcgaaattaa
     1501 aaccgccttt atcgcttttg tgcttgaaaa agtcaaaaag caaaaatgca ttcgtggttt
     1561 ctataagaaa acatgagtgg taaatatatc gaattttcat agcgcctcca tttataaata
     1621 tttgagtttc ttaattataa tataatcttg aactatgagc aatactataa ttaaattact
     1681 ggtcatttga ggactatcct tcaaaagatt tctctcaaag agccatcctg aatttaaaga
     1741 tgtttccaca tacctttaat aaaaagtgtt cgcaggaaaa ataatgtttg taattacaat
     1801 atttacatag tataatcaga agttgataaa aatagaagag gaaaaaggtt aatgaaaatt
     1861 gaaaatgtag tattgaatga cagcattcaa agagctttag acgatatggg ctttgaagaa
     1921 agcacacaaa tccagcagga gacgattcct ttaataatgg aaggaaagga tatagtagga
     1981 caatccaaca ccggaacagg aaaaaccgca gcttatgcaa ttcccattct ggagaagata
     2041 gataaagaat taaaaatgcc acaggcactg gtacttcttc ctacaaggga gctggccgtc
     2101 caggttgcca atgaagtaag gaaaatcggc aaatatatgg atgggatcaa gacagtaact
     2161 gtttacgggg gagcagacat aaaggaccag ataaacaagc tgaaggcagg agcacagatt
     2221 attgcaggaa ctccaggcag gattattgat ttaatcgaaa ggcatgtaat aaagctcggg
     2281 gaaataaaaa tcactgtact ggacgaagcc gatgaaatgc ttaagatggg cttcagagaa
     2341 gatattgaac tcatactggg taaaattgac catccggtgc aatctctgtt gttttccgca
     2401 acgattcccg atgatataaa aaagattatt aataaatttc tgcattctcc cgtatcggta
     2461 aaggttttaa gagaaggaat aaccgcaaag gaagtaaagc aaagctattt tttagttaag
     2521 cattcagata aggttgaagc attggcaaga cttatcgata catacactcc aaagctcact
     2581 ttggtatttt gcaatactaa aaggggagtt gatgaattgt atgatcagct ggtcgacaag
     2641 ggttataact gcgataaaat tcacggtgat ataaaacagt cacaaagatt agatacttta
     2701 aataagttta ataatggttt gattgaaata ctaattgcta ccgacgtggc tgcaagagga
     2761 cttgatatca aagaggtgga actggtaata aactatgaag ttccttcaaa ggaagactat
     2821 tatgtccacc ggataggcag gacagggaga gcaggaaaag aaggagcatc ctttaccctg
     2881 gcaagtgcaa aagaattaag aaaaatagag gatatcgaaa aatataccaa aaaatcaatc
     2941 cgtaaaagaa ccatacctac tgtagataaa gttaatgaag ttaaacagga taaatttatc
     3001 aggggtattg taagtgtaat tgaaagcggc gacctgggag aaaacagaga actggcagga
     3061 aaacttatgg ataagggtta tgatgcagag actgtaatag ctgccatgat taaaaagctt
     3121 gttaaatttg acttgtcgga ggaaagagat ttaaatgatg ttcttccgga aagaagaaaa
     3181 agaggagaag tcaagcttga aaatacggaa aggtttcatg ttaatctggg gaaaaagcag
     3241 ggtataaggc cgggtgatat tttaggagct gttgcgggag aatgcgatat tccgggatat
     3301 gatataggag agatagaagt attggacaat ttttcattct tcaccgcttc taaagaccat
     3361 aaacacagga ttcttgacag aatgaaccgg tcacagataa agggaatgga tgttgccgta
     3421 gagttatcaa aggaaaagaa atcgagattt gcaagagaga agtcatcaga cgataaatca
     3481 ttctggaaaa gaaagaaaag ataactatat gcgcctgcat aaaacataag actgttacga
     3541 acaattgtgt caatttaaaa ttattgttga caaaaaaaat taccggtaat ataatggtca
     3601 aaatgaactg tgatggatcg agtaaaacag acacagctta acagagaggg aagaagggtg
     3661 gaaattccca tgcatgactg ttcgaaaact aatccggagt taagacagaa agcgaaagca
     3721 gcctaatgtc ttggtatgga tgccttaaat cctttgagaa gcaactaagg tggaaccacg
     3781 atatctcgtc cttatacatg aggacgggat ttttatttta ttcattcacc agggtgatgc
     3841 ataaagaaat aaatgataat aggaggatat tatgattaaa gtaacatttc ctgataaaag
     3901 cgtcagagaa tatgaaaaag gaatcaaagc agttgatgtt gcaaaaagta taagcgacgg
     3961 actggctaga aatgtggttg gagctgttta taatcgtaaa tcaatagggc ttcaaaatac
     4021 attgaatgaa gacggaagta ttaaatttct aaaatttgaa gacaaggaag gcaaggatat
     4081 attctggcat acttcatcac atattatggc acatgccatt aaaagactgt ggccggatgc
     4141 gaagcttgca ataggacctg ctattgaaaa cgggttctat tatgatattg atttagaaca
     4201 caggtttatc caggaagact ttgaaaaaat cgaagccgag atgaagaata ttgtaagtgc
     4261 tggttatgaa ctggaaagat ttgaacttcc aagagaagaa gcaatcaatt tcatgaagga
     4321 agcgaaggaa gactacaagg ttgagttaat tcaagacctg cctgcagatg ctgtaatttc
     4381 cttttacaag catggtgatt ttattgacct ttgtgccggt ccccaccttg aaagcacaaa
     4441 aaaaccaaag gcttttaaac tgctgagcat agccggagct tactggaggg gaaatgaaaa
     4501 caataagatg cttcagcgta tatacggaat ttcatttgaa aaagctaagg atctggaaga
     4561 ttatctgaaa atgatggaag aggcaaaaag gagagaccat agaaagctcg gaaaagaatt
     4621 agacctgttt atgatgtctg atgaaggtcc cggttttcct ttcttcctgc caaagggtgt
     4681 tgtagtaaaa aatgaactta tgaagtattg gagagaaatg cataaaaagg caggttatgt
     4741 ggaaatagaa accccattga ttttaaacag acacttatgg gaggtttccg gtcactggtt
     4801 cacttacaga gaaaatatgt atactacggt aattgatgaa caagattttg caataaagcc
     4861 aatgaattgc ccagggggga ttttagttta caaaaatgca atgcattcct acaaggattt
     4921 ccctatgaga gtggctgaag caggtagggt tcacaggcat gaattatcag gtgctttgca
     4981 cggattaatg cgtgtaagag cctttactca agatgatgct catttattca ttttgcctga
     5041 acaaataagg gatgaaataa aaggtgttat taaattaata gatgaaattt ataaacaatt
     5101 cggattttcc tacaaattgg agctttcaac aagacctgaa aagtttttag gtgaaataaa
     5161 agactgggat ttggcggaag catcacttaa agcagccttg gatgaactga atctggctta
     5221 tgtaataaat gaaggggacg gggctttcta cggaccaaag attgacttcc acctgacaga
     5281 ctgcatagga agaacctggc agtgcggcac cattcagctt gattatcagc tgccacagcg
     5341 ctttgaaatg gaatatgtag gagcagacgg acaaaaacac agacctataa tgattcacag
     5401 aactgccttc ggaagcattg aaagattctt cggaatactt attgagcagt atgcaggggc
     5461 attcccaaca tggcttgctc cggtacaggt aaaaatactg cctatttccg ataagttttc
     5521 agactatgca aaagaggttg agaacaaact gaatgcactt aatataagag ttgaaacgga
     5581 ttcaagagca gaaaaaatag gctataaaat aagagaagcc caggtggaaa gaatccctta
     5641 catgtttgtg gtgggagaaa aggaagccga aagcaatact gtttctgtaa gagaaaggca
     5701 tcagggcgat attggggcaa tgagcataga cgaaatcagc agcattattt tagacaaaat
     5761 taaaaacagg gaaaatgact ttccccaatt gctttaacaa tatatgatat gggaatacac
     5821 ccgatttaag agtggttctc ctgagaacag ttaaaaacgg ggcttgaaat attcagcctc
     5881 gttttaattt acagccggcc cctttatttc tttcataaga tttttcaaga aactacatgc
     5941 ttgtgattta gcttttctcc gacaaaatat atattgaact ttgccggcac tttatatatt
     6001 atgtagatat aataatttat ggaggtataa gcaaatggga acagtttact cggatgctca
     6061 atatcatata gatatgaagg aagggaatgt gggcagatat gtaatattgc ccggagatcc
     6121 taaaagatgc agtaaaattg ccaagtattt cgataatccc gtattggttg ctgataatag
     6181 agaatatgta acatataccg gatatcttga cggagtgaag gtaagcgtaa catcaacggg
     6241 aatcggcggc ccttccgctg cgatagcaat ggaagaactc gcaaacatag gtgcagatac
     6301 atttataaga gtaggtacct gcggaggcat gagcgttgac gttatgggag gcgacctggt
     6361 tatagccaca ggagcaattc gtgctgaagg cacaagcaga gaatatgcac ccgtagaatt
     6421 tcctgctctt gcagacttta aaattgccaa tgccctggta aaagccgcag acaatttaca
     6481 taaaaaacac catgttggtg ttgttcactg caaggactct ttttacggac agcacgaacc
     6541 ggaatcaaag ccttcaagct atgaacttat gaataaatgg gaagcatggc tgagaatggg
     6601 aactttggca tcggaaatgg agtcagctgc attatttaca gttgcagcat acagaaaggt
     6661 gagagcaggg tctgtatttc ttgtagttgc aaatcaagag agaagacggc tgggtctcga
     6721 ggatccccag gagcacgata cggaagctgc aattgcgact gcagttgaag cgctgcgaat
     6781 actaatcagg gaagacaata aataaagaag ttataaagta aagaggtatt tcctcatacc
     6841 tctttatttt tatacattta tagttttcca ttttcctgaa ttatacctga tgagaacaag
     6901 tgccgaccgt atggtttgat cgactataaa tgcaaaccag gctccatcca gccccatatt
     6961 ccacgcatta atcaagtaca tagccaaaaa cggtcttatc agaagcactg ttatgaaagt
     7021 aataatagca gtagctcttg tatctccggc acctcttaag gagcctgcta aaataaactg
     7081 tgaggactgc aggggctggg tgaatcctac aaacatcatt attcgagccc cctgtgaaac
     7141 tatggccggg tcatcattgt agagagaaac tatctttccg ccccagaata taaaaattat
     7201 tcccagtagg attgaaacag acattccaag tcttcttgtt ctgtgaacat aggcatgagc
     7261 catgtcaggc cggtttttac ccagactctg acctgtaagc gaagtagccg atacggcaaa
     7321 accctggccg ttcataaagg acatggcctg gatgttcatg catacctgat ggaccgcata
     7381 tgcaacagtt ccaaggcctg caatagttct tacatatatt atgactcccg cacgcatcaa
     7441 aagctgttcc agcatggcag gagcacctat ggcaactatc tgcttaaggg tgggaggatc
     7501 aggtttaaaa ccgtcattaa aacgaagatg cagatattgg tctcctttca tgatatatat
     7561 aagggcgaaa gcaaatgcaa gaaattggcc gattatggtt gccagcgaag cacctgccac
     7621 ttccagcctt gggaatccga gattcccttc tatcaataaa taatttaaaa tgacattcac
     7681 cacatttgat gctaaattat atatcatggc tgttcttgag tttccaacac ctcttaatgt
     7741 tgctgtgatt gtggatgtaa gagccatgaa tggaaagccg atcatttgaa ttttaaaata
     7801 ctgggtaccg ccctgcagcg ttttttcttc ggcagcaccc ataaaaagta taaatttttc
     7861 cgcatataca taaccgataa ctgaagaaac cgctgaaaat atcaaagtaa gaacaagtgc
     7921 ctgcctgagg attaaatttg ctttttcttg ttctccggct cccttgtacc ttgctgccat
     7981 tgcagtagca ccaacattca tagccatgaa aaatgtcatt aaaagaaatt tcggctggac
     8041 ggtaagacca acagaagtca gagcccaggc tcccaaccct ccaaccatca tcaggtccac
     8101 catggatgca agctgagtta aagtaagttc cacaacagat ggccacgcta tttttacaat
     8161 atcggagtaa agcgattttg aagttacctt gggtggaagc tgatgtttct ttatatcttc
     8221 tatttgaata tccctgccgt cttctaccag atccagggca agactataag atttgaattt
     8281 ttttactgtt tccattattg ctccttttat caaccgtttt tcttaaaatg gactcggaaa
     8341 aacgggtttt gtccatacaa aaggccgcag agctgcgacc tttatttggt ttatcccgct
     8401 aatattattg tcaattaata atatcacgaa aaaatttcaa tgtcaaataa tacgaattca
     8461 aaaaaatgtg aaaacccttg aagaaagatt taaaaataaa aaaattttat aaatcatctg
     8521 aaattaagaa gtttactcaa agaatttttc atgttaatat tgaattactg taattattag
     8581 gttaatattt atttttacat atgagaactc caagacatgg tattagcagg ttgatttaca
     8641 gttttataaa actagtggag gtattgttgt gagcatgtta ttatcaattt ttcttattgg
     8701 attttttggt tatatgttag gcggcataaa gatttattcc atagagctgg gcacagctgg
     8761 tgtcttgttg gttgcattgg tttttggtca ttatggtatc gttactcctg atattgtccg
     8821 cgaaatgggg cttgtatgct ttgtgacttc ggttggtttt gttgcaggtc cgaatttctt
     8881 cagaaacttt aaacaaaacg ctgcatccta cgtgctgatt ggtgtattaa tgattatctc
     8941 aagtgcttta agttgtgtat tgattgtaaa ggtaagcggt ataagtcctg ctcttgctgt
     9001 tggattattg acaggtgcat tgacaagcac accgggattg gcagcagcaa ttgaagcagc
     9061 tggggagtat ggtaacctgg cctcaatcgg ttacggcatt gcttatcctt ttggcgttat
     9121 aagcgttgtt ctttttgtcc agttaattcc aaagattttg aaagctgata tggctaaaga
     9181 gcgtgaggtt cttgaagcag ttactgacat tactaaaagc aagtcaatat ttcagaactt
     9241 atattcattt gacaatgcgg ggttcttctc tgtctgcatg gctgttgttt tgggtatttt
     9301 tcttggcagg gcaagtattc ccctgccggg cggtgctaaa ttttcactcg gaaattcagg
     9361 agggccgcta attgtgggct tgatttttgg acatttcggt catataggtc agttcaatat
     9421 ttcagttaag aaacctgttt tggaaacctt gcgtgaactt gggcttatgt tgtttttaat
     9481 tggtgcgggg acaaatgcag gacaagggtt tgttgaaatc cttaaacagt atggtttgat
     9541 gttattttat tacggagcca taatggctat aatccccatg ataataggat tttacttatc
     9601 aggcaaaatt ctgcaagtag gtcttcttaa tactctaggt gcgattaccg gcggtatgac
     9661 cagcactccg gcactgggtg cacttattga agcagccaaa actgatgatg ttgcatcttc
     9721 ctatgccgca acttatcctg ttgcattagt gtcggttgta ttggcatcac agtttataat
     9781 catattattt ggttaattca taaattcttt agagagcaat aatttaagaa aaaattattt
     9841 ttaattatta aaataccttt ttatatttta atgcaatttt aatatatccc taaaacataa
     9901 ttaattatca ggtggtatct tatgaataag acaaaaataa aaattaaata tataggaggt
     9961 aacatgaaag ttacattgga acaaatcgat gaacttagga acagagtaaa tgtaagctac
    10021 gaggaagcta aaagaaccct tgaaaaaaat gacggagact taataaaatc tataatagag
    10081 ctggaaaaca aaaaaggaag gaaaccggaa cagagaggga gctttacaag ttttgcagac
    10141 agacttttgg ctttgaaaat gtctgtaaaa aacaaagagg gaaatacagt tttaaatgta
    10201 cctgtggtag tagttttaat cacctttttg atgtcatttt ggacggtaat cttcttattc
    10261 gtactggcag tgctcacatc ctgcaaaata gatgtctaca gggataaatc aacaaatgta
    10321 aacagcataa agaaaaacat gcaggacacg gtagacaaga taaaggttaa gtcagatgaa
    10381 atctttaagg acgagaaaaa accggaagac acaaaagatg actcggaaaa tgaaataatc
    10441 atcgaataaa gaataaatat caggtctttg acctgatatt tattttaaca ctgaaaacaa
    10501 gcttaggggt aatttgaatt aactgattga taaacaagaa aaaaaatagt acaataaaac
    10561 aaaaacgagg tttttatggc taaaaaaaag attttgattg ttgaggacga gcaaaagata
    10621 gcacggtttt tagagcttga attaaaatat gagggctatg atgtaacaat agccaataac
    10681 ggcagagagg gctttgaaca aggtaaatcc ccggacattg atctgataat acttgatttg
    10741 atgctgcccg gattaagcgg tattgaagta tgcagacgct taagacagac ctccgatgtt
    10801 ccaataataa tgcttactgc aaaggatgat atatcagaca aggtaacagg tcttgacata
    10861 ggagccgatg attatatgac aaaacccttt gccgtggaag aattgctggc acgaataaga
    10921 gtattgttaa aaagaaagta ccataaaaac gatgatgagg accttctcat agtcggcaag
    10981 cttaagctgt ccaaaaacaa ttacaaggtt gaatacgaca atgataatat cgagcttacg
    11041 aaaaaagaat ttgagttatt ggaatacctt atgcggaaca aaaacatagt tctgtccagg
    11101 gaaaaaattc tggacaatgt ttggggatat gactattatg gagacactaa cataattgat
    11161 gtatacataa gatacataag aagcaagata gaccagaaat atgatttaaa tcttattgaa
    11221 acagtgagag gagtaggcta catcataaga gattgaagga atacaaatgg ttaagacaat
    11281 aatcggaaaa cttttaaaca taattctcta tccctttaga aagattgtag aagctttggt
    11341 aagccttata aaaaggctta gattttctat tgccttcaaa atctcaacca catatttaat
    11401 actctacatt ataataatta tgacggttgc tcttacaacc tctgtcggtt atttaatatt
    11461 tgagctgcag aaatttgaag agtcaactgc agcagaggat tctgcaataa taaaaagcag
    11521 ctttcctgat tttaataata tagatgtaag cggaaagaca tataattcct ttgaaatatt
    11581 tgacagcagt ctgaatacat actattcatc gggaaagccc ggcaggtatg cagacagcct
    11641 tatagtaatt gcggaaaaaa taataattga caatgaatat gttcattcgg aaagcttcat
    11701 gaaaaacggt tctatgtatt atatgaatgt aaactaccct atggaagaca tgctcagaga
    11761 tgcctggaag ataagcttta tggttttaat atcaggggcc ataggtctta tgttttttgc
    11821 ccctattgtt tcaagcacca gctacaagct tatcggtccc attaaaaaca tgactgagat
    11881 aaccaggact ataaccgtga ataacattaa caccaggctg gatgtgaagg gaacagagga
    11941 tgaattaaag gaattatccg aaaccttcaa tgaaatgatg gacaggatag aaaggggtta
    12001 caaaacacag cagcagtttg tttcagatgc ttctcatgag cttagaacac caatagcggt
    12061 tataaagggt tacgtaaata tgcttgacag gtgggggaaa aatgacaagg cagtccttga
    12121 tgagtccatt gaagcaatta aaaatgaaac cgacagtatg cagaatttaa ttgaaaagct
    12181 attgtttata gcaagaagcg acagaagcac actcaatttt actaaggaag atttcagaat
    12241 atctacagtt ctgaatgaaa ttgaaaaaga aacaagaatg attgacacaa cacacaaact
    12301 atattttaaa ttttatgatg acgcaactat ttacggagat aaaatgcgta ttaaacaagc
    12361 tgtgagaata cttcttgaca atgcgatgaa atatactcct ccgggaaaat atattatggt
    12421 ttcaggttac attgaggatg aatattatgt gataaaggtt gaggatacag gtgaaggaat
    12481 tcaaaagaat gatttggata aaatatttga cagactttac agggcagaac agtcaagaag
    12541 caagaagata agcggtcatg gactggggct gtccattgca aaaataattg tattaggtca
    12601 taaggggaaa ataaaggtta agagcacacc gggaaagggt tcggaatttt ccctgctctt
    12661 tcctcatctt gacaactgat tcaaattcac ttaaattttg acataaaaca acaaaataag
    12721 ctagagatat atcctttcct tagtagtata atagcttgta cattatgcag aaaggatgat
    12781 tttgtgaata ttcagtttat agaaacaaca aatgtgctga ctatcaagct caagggagaa
    12841 ttagaccacc acagtgcaga tgaagcaagg gttttaatcg atgaaaaaat aaaaaatgaa
    12901 aattttaata aactggtaat agatttaaag ggacttgatt ttattgacag ttcaggcatc
    12961 ggttttgtca tcggccgata caaggtcata agaaagcgca atggtgtgat cgaaatagta
    13021 aatgccggca aaaagataag aaagattttg gacatgagcg gcattggaaa aataataaaa
    13081 ataaaatagg gggacaaaat gcataataac tacgtatata ttaaaattcc aagcttgtca
    13141 gttaatgaat cctttgcaag ggctgctgtt gcagcatttt gctcaagctt gaatccaacc
    13201 atgaatgaaa tatctgatat taagacagca gtatcagaag cagttacaaa tgcagtagta
    13261 catgcctatg aggacgaaat aggagaaatt gaaattcaat gcagaatcaa ggggcaggta
    13321 gttgaaataa tagttgagga tttcggctgc ggaatcgcag atgtggaaaa ggcaagagag
    13381 ccgttattca ctacacgtcc ggaactggaa cgttcgggaa tgggcttttc ggttatggaa
    13441 tctttcatgg atgaactggt tgtattgtcg gaaaaggatg ttggaacaaa ggtaatatta
    13501 agaaaaaaaa tatccgagaa aaaacttaag acattaaatg aagaaagcga tgacgaaagt
    13561 tcagaagatg aagaataaat aatttttacc gcacagcagg ttttagaatc aatataagga
    13621 agtaaaattt aacaaatttt gataaagaag gcactcttag tgcttttttt tagtattcaa
    13681 attttttttt tgtaaataat actcttgagg tgatttatat gaaaaaaaag ttattttttt
    13741 tagttataat tctaattgtc ggtttgactg ccttatattt aggcaacaat attgacaggt
    13801 ctctgaaaag aataccggaa agcgcagttt tattatagta aagttattat attatactct
    13861 gcaggaggaa gtatgaaata caataaaaac aactataaaa aggtagttga tgaatttacg
    13921 ccaaaaaata aaatacttaa aaattgcctt atggccttta ttttcggagg aactatatgc
    13981 ttgataggag aaattatcaa tgattccttt gtaggctggc ttggaatatc agatgatgaa
    14041 gcatcaccca tgacatccgt ggtgttagtc ggcttgagtg cagtgttaac aggtttagga
    14101 tggtatgaca aactgggtaa aatcggagga gcaggaatgg tagtacctat aaccggcttt
    14161 gcaaactcgg tagtttctcc tgctttagaa tataaaaggg aaggatttat ccttgggaca
    14221 gctgccaata ttttcaaact ggcaggacct gttttgttat ttggatattt ttcttctttc
    14281 attgttggtt tattgagcat gttgtttaag taaggagaaa atatgaaaaa gacgggaaaa
    14341 acttttataa tcaaagatga tgtaataata agagatactt acaccatagc aggcagcaag
    14401 gaagggcagg gtcctttaaa gggcagattt gacatggtac tggatgatga tttatgggaa
    14461 gaagattcat gggaaaaatg tgagctgaaa atgcaaaaga ccgcagtaga aaatttaaaa
    14521 aataagaatt cattaataaa tgaaaatatt gatcttttga tttcaggaga cttgcttaat
    14581 caaatcacat cctcctcctt tgctgcaaga gaattggaac ttccatacat aggagtttac
    14641 ggcgcctgtt ccaccatgac gttgacaatg ggattaggag ctttgattat tgacggggga
    14701 tttgctgaaa atgtaatctg cgttacctca agccatttct gcagtgccga aagacaatac
    14761 agactgccgc ttgaaatggg aggacagcgt catatgtcag cacagtggac agtaaccggt
    14821 tcagctgcag tatggctttc aaacagtggg aaaggaccta aaataaaaca tgttaccttt
    14881 ggaaaaattg ttgatttagg tgtaaaggat gcaaacaaca tgggtgctgc catggcaccg
    14941 gctgcctatg attcattgag caggcacctt caggatacag gtattgatta tgatttaatt
    15001 gttaccgggg acttgggaaa agtggggaaa agcatagtta ataaaatgtt tgatgaggat
    15061 aataagagta ttgaatcaaa atacgatgac tgcggtacta ttatttttga tgtaaataaa
    15121 caggatgtac atgccggggg aagtggctgc ggctgttcag caacagtatt cggctctttt
    15181 ttatataaga aattattgaa taatgaaata aaaaaacttc tgtttacatc aaccggagca
    15241 cttcattcgc ctactgcgac tctccagggt gaaagcatac cgggaattgc ccattgtctg
    15301 ggca
//
LOCUS       DAOPUT010000038        15109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_4008_length_15109_cov_19.692773, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000038 DAOPUT010000000
VERSION     DAOPUT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15109
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4008_length_15109_cov_19.692773"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PKK75_04835"
     CDS             complement(<1..1145)
                     /locus_tag="PKK75_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNZ82955.1"
                     /translation="MILTEEQKNHYKRHIIVPEIGEEGQKKLLDSTVFVYCENTDSLR
                     PLAYYLAALGTGRIFCCLKDKGDGDSLFAEVIDLNSDTKIDYLNNETRKSHPQNRQNR
                     NSENEKNRPQNYRIVLGSLSFIKSLAAELLSEDFIPTIVSVNGEWRGTLQSFQNEDLF
                     KNFMDAVKTENAEYKTSTNNNTKIKSPVLLANALSAVLCTIEGVKLCLGIGSIKGDML
                     CYDLLSMEFSQTKSTVNLFEFLNEAYTFDLKKLSNARVLIAGVGGLGCPAAYGVAISG
                     VNTLGLLDFDEVEMSNLNRQILHSFSRIGMPKADSAKFILKKLNPDINIKTYKTELTN
                     DNAEEIFSQYDLVIAAVDNIPARYLINDTCLSLNKPFSEAGVLRLDGT"
     gene            complement(1240..1977)
                     /locus_tag="PKK75_04840"
     CDS             complement(1240..1977)
                     /locus_tag="PKK75_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82956.1"
                     /translation="MKKILIVLLILIGAVGIFYRQPEYYQKGHIRIEQNIYDLYEKDN
                     YLYIKTDKVFKKLIYEELIDHRIYNIDSDENDEILVINRHATEEQGSDFIIYDIEEGN
                     GSMKAKEIYRQDFSDIKPWKVDACNLDNDGETDIFIGVHKDTVFYKDVRNRPFFYSWD
                     GEGLNKKWLGSFFTDWDLVDIAFGDYFNLGYDLAAVLEKNNDSYRIGYYKFVGFGFIN
                     MDTEEIYTNVKSIETVRDGGIDTLVLN"
     gene            complement(1979..4321)
                     /locus_tag="PKK75_04845"
     CDS             complement(1979..4321)
                     /locus_tag="PKK75_04845"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNZ82957.1"
                     /translation="MNIKKFLVLLLCIFLLASCSYNNVPEEPEPIEEEPGIIEVNIPP
                     AWNMNMEEINVDVPFSVPDVTPLVEKYYVDENLSNLVNSGQYTGFTKNQIKSIYEDGF
                     VVLKPSCDYLKMHHIYEYPMYKESPVFITVDSALHIYHIYYSNSLKLLEVSSLYDKLE
                     SLTKNMLDESLKAYNNPEYADLKAELKFVSAYFLTGAKLIGAGVEGLEIPYEVAALAG
                     DEIKLIEEAFDFANSPIFGKDLDYSQFTVRGHYTGNEKLEKYFKTMMWYGISGFPVFD
                     ETKEDPVLDMDSLTKSMIITCLALENEKNFTDFENIYTTTALYTGISDDLGIFEMRDL
                     ITKVYGQAPDLNVFKDDSYQDKLLEEALLLPEPKIQPKYTLVSTPAGRQFRFMGQRYS
                     FDAEVMQNLIEPILRPVPSGLDVAASFGSTRAEEILDTYYKPKEEWDKYEENLNEMRE
                     KQTEITEDEWKSDLYKGWLWSIKSSAVSYEDREGMPFFMRNEKWTDKNIHTALGSYAE
                     LKHDSILYMKQSGAEMGGGVEATIPYNYVEPNVEVYAKLKWLAENTKEQLAARNMLKD
                     DIGRTLDQIIEIQDTLLNVSIKELTNQSITEEENLALYRYGGIIDNLVQILQMNLRRN
                     EVDTSNDYTTALIADVSTIAPNNMFPKGSYLELGNGLPCEIYVVCQTNGNTYLAKGAL
                     FNYYEFISDKRLTDQEWQTMVGVKKVAMVEDKESNKYVPMDIYDEAGNKLIGEDTFYF
                     EDIIITGPSENMVDKPAWTESFISPEENNITINDMEIYWE"
     gene            complement(4437..4703)
                     /locus_tag="PKK75_04850"
     CDS             complement(4437..4703)
                     /locus_tag="PKK75_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNZ82958.1"
                     /translation="MNDKIYEFDAVILKVPDIDGAYIEFPYDVRKEFGKGRVKVLAAF
                     DGEPYEGSLVRMGTPGHIIGIRKDIRAKINKQPGDTIHVTIRER"
     gene            complement(4830..5507)
                     /locus_tag="PKK75_04855"
     CDS             complement(4830..5507)
                     /locus_tag="PKK75_04855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ82959.1"
                     /translation="MKKIDTIIFDLDGTLLNTLEDIADGVNYILKKYGYPKRTCKEIK
                     CFVGNGAARLLEQALPDGLSTRNFGSILEEYKKYYLDHNNIKTSPYEGIMDLLEELSV
                     RKYKLAIVSNKNDKNVKSLNKIYFEDYIKTAIGQSDNINRKPAPDMVYKAIDELSADK
                     ERTVYIGDSEVDILTAKNANMHCVCVTWGFRDKEFLKDQGAEYLIDEPHELLEFLGGF
                     SVPEEAK"
     gene            complement(5553..5891)
                     /locus_tag="PKK75_04860"
     CDS             complement(5553..5891)
                     /locus_tag="PKK75_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ82960.1"
                     /translation="MSRYFIEEIERLPLADIPIRGVSAYLSQGVNHQIIFMEFDEDAD
                     LPEHSHESQYGIVLEGKIELVINGVKNEYVKGDRYYIPAGVSHYGKIYAGYADITFFN
                     EKNRYRELTP"
     gene            complement(5915..6526)
                     /locus_tag="PKK75_04865"
     CDS             complement(5915..6526)
                     /locus_tag="PKK75_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HNZ82961.1"
                     /translation="MTGLILKDLLNLKKQAKVYLILVIFYFVYGAVFENSSMFSSMMT
                     MVAVMIPITAMSYDERSKWDRYALTLPISRVGIVVSRYLLGVIFIVIAFVLSMLVNLV
                     FSKMYSTESILINLAALSAGITVMSVIFPLLFKYGVEKGRILMMLVFFAPIAILMIVS
                     KLGVPMPDEETIKSFIYLSPFIAAVIFLISFYISLSIYKKKEF"
     gene            complement(6526..7371)
                     /locus_tag="PKK75_04870"
     CDS             complement(6526..7371)
                     /locus_tag="PKK75_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82962.1"
                     /translation="MEYALRLENVTKEYKNFKLDHINIALPKGCIMGFIGENGAGKTT
                     AIKLILDLINRDDGKITILGKDNRTEMNAIKENIGVVLDESSFPENLNAAEINHILKK
                     IYKTWDENKFFSFLDKFSISHNKSVKDYSRGMKMKLSIAVSLSHDSKILILDEATSGL
                     DPIIREEILDTFLEFIQDESNSIFVSSHIISDLEKICDYITFIHKGKIIFSEAKDDLL
                     YDYGILKCSVEEFENIDKSIVKGMRKNKFGIEALILKNEISGNYIVDKASIEDIMIFI
                     IKEQV"
     gene            complement(7373..7747)
                     /locus_tag="PKK75_04875"
     CDS             complement(7373..7747)
                     /locus_tag="PKK75_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ82963.1"
                     /translation="MNIIISNSSSQPIYEQIVSQIKSLIITGELNEGEALPSMRLLAR
                     ELRISVITTKRAYDELEREGFIVSFTGKGSFVAGKNIGLIKEQLLKSIENHMQEIVKL
                     SGNCGIELNELIEMMKLIYEGE"
     gene            7945..>8609
                     /locus_tag="PKK75_04880"
     CDS             7945..>8609
                     /locus_tag="PKK75_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ82964.1"
                     /translation="MKISKQEFEEEKESSPKFKYTDVNSKFFDKWIDEGWEWGKPVSH
                     EIYEKAKNNDWFVLLTPTKPVPKEWFCEMKDSKILGLASGGGQQMPIFTALGAKCTVL
                     DYSEKQIASEIEVAERENYEIKTVRADMTKPLPFEDETFDLIFHPVSNCYIEDVLPVW
                     KECYRVLKKGGILLAGLDNGINFIFDDDETKPVCKLPFNPLKDERLYQFSIENNWGIQ
                     FSHS"
     assembly_gap    8610..8709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8710..9093)
                     /locus_tag="PKK75_04885"
     CDS             complement(<8710..9093)
                     /locus_tag="PKK75_04885"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNZ82965.1"
                     /translation="MDKGYVQIYTGNGKGKTTAAFGLALRAAMSGKKVFIGQFVKNMK
                     YNETRIENYLDNIQIKQFGRGCFIYEKPEDADKEAAKKGLDECGEILAGGEYDLVILD
                     EINIALYYKLFEVSDVINVLYNKAEK"
     gene            complement(9110..9994)
                     /locus_tag="PKK75_04890"
     CDS             complement(9110..9994)
                     /locus_tag="PKK75_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ82966.1"
                     /translation="MKKYKVWGLLVLCVLFWAGNYIFGSLVVTELTPVGLTFIRWLFA
                     SVILIAVGHKIEKPSWKEVYKQWPKMVGLSLFGIIGYNVLLYYAFYYTSPLNAALVNS
                     FNPGFIAVVSALYLKEKITKHHIVGLVISFLGVLIVLTKGNLMQAFTMKYNTGDFLII
                     VAVISWTVYSLIGKKVTTVPPITSTGVSGLIAAIILLPFAMNNGVDYLNISPLAFTAV
                     IYIILFPSMLSFVFWNIGVREIGAVKAGIFMNLNPVFTAFISFAMGQEITWVQITGGL
                     LVFAGVYITTAFKGNTIR"
     gene            complement(10649..10876)
                     /locus_tag="PKK75_04895"
     CDS             complement(10649..10876)
                     /locus_tag="PKK75_04895"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNZ82967.1"
                     /translation="MSDEKLLLSNLNTMEAEIIISKLKSYGIPALKKTKGTGELMEIY
                     TGVNMYGIDIYVPEEMAELAEELLKPENEQE"
     gene            complement(10973..11866)
                     /locus_tag="PKK75_04900"
     CDS             complement(10973..11866)
                     /locus_tag="PKK75_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82968.1"
                     /translation="MKKKFLIVLLCIAAMFAFIPPLAYGDVGPKPSVIVNFEGLQGKT
                     YYTTLLSETSSTGPYSAVGRFEGDRRYSEDEEDYEIWNKFVDYQDEDGYYFLQYFSDC
                     TETQEFKWTYYPPQKFKILLYFPELDSFAVSDVYERYAFASYYTVDAENMELTPLKVH
                     EGITAEKSYNFTREIISLFLRIIATIAIEILVALLFGYRAKKQLRIILITNIATQTIL
                     NIMLNTVNYYYGGLAFLLSYILAEIVVFLIEAGVYASFLTKDGTGRLTRKSYSVLYAF
                     VANVVSFYLGFQIARLIPGIF"
     gene            complement(11927..12583)
                     /locus_tag="PKK75_04905"
     CDS             complement(11927..12583)
                     /locus_tag="PKK75_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017151674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ82969.1"
                     /translation="MKYKCLILDHDDTVVKSTPAIHYPSFVEALKSLRPKVELSLNDF
                     VNYCFAPGFVELCTDIMKFNETEQEYQYKIWKDYVNENSPDFYPGFPELIKDYKSSGG
                     IICVATHSESRHIIRDYTLHCDGIIPDLIFGWDMEESKRKPNPYPIVETMKSFNLKNT
                     EMLVLDDLKPGLTMARSCSVEFAAAGWSHGVPEIEDYMRNNSDYYFSTVDEFREFILG
                     "
     assembly_gap    12596..12695
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12696..12992)
                     /locus_tag="PKK75_04910"
     CDS             complement(<12696..12992)
                     /locus_tag="PKK75_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ82970.1"
                     /translation="MKFLWTTLLVDNMDESLKFYEDIVGLKLADRFQAGPGMEISFLG
                     EGETKVELICSEKLKKLDAGNRVTLGFKVESLDEKINFIKEKGIDIITGPVQPNP"
     gene            13196..13816
                     /locus_tag="PKK75_04915"
     CDS             13196..13816
                     /locus_tag="PKK75_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNZ82971.1"
                     /translation="MTRYSAESNLHNKTMTLTGNGEMDIAPDTVLIRLGVQTTGENLS
                     QIQAENARISQSIIQTLQRMGVTDIKTAQYSIDKVYDYVEGRQIDRGFSVRNILEIRT
                     NNLEAAGSIIDASVNAGSNVVDLITFDVSNREYYYRQALNMAIGNAIQKAKSIAVNLG
                     ISTTPVPVNIVENTAMPFPVQREFAATPIMPGTIKVEANVTVDFVY"
     gene            complement(13813..14388)
                     /locus_tag="PKK75_04920"
     CDS             complement(13813..14388)
                     /locus_tag="PKK75_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HNZ82972.1"
                     /translation="MDKVTLWTRQDKRSYDNLEKDGVIRITRGHLEEKFDLISDHIIY
                     LYKWFVDEAEKRVPKPEGVEFPIWCSISEEYMLRPTLYEIVYVLEVDKSDIVYFDGMK
                     WDYVLNHHYVPKDEKDAEEYLKDIESKGYKNSYSFIDEKTAHFYPHERKRVMDSWARV
                     FEIDKWDIFRIQANIWEIRPEMIKDILRVDS"
     gene            complement(14503..14991)
                     /locus_tag="PKK75_04925"
     CDS             complement(14503..14991)
                     /locus_tag="PKK75_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82973.1"
                     /translation="MFIRRNQLYIIIAVIVFLMAAFIIYSIYNNDNSKDESKDVIVLV
                     EDFGSTLKNVSLLAPRDLVIEAIEANYSPYVTDELMQSWLAHPRRAPGRTTSSPWPER
                     IEITEVVRLSEGEFSVKGRIVEVTSSEAGGGAAVYRPVELIVRKVGNKWLIDDIRVGN
                     YE"
BASE COUNT         4562 a         3026 c         2224 g         5097 t
ORIGIN      
        1 gtgccgtcca gtcttaaaac tcctgcttcc gaaaaaggtt tatttaatga aagacaagtg
       61 tcatttatca gatatcttgc gggtatgttg tcaactgctg caataaccag atcgtattga
      121 gaaaatatct cttctgcatt atcatttgtc aattccgttt tataagtttt aatattaata
      181 tccggattta gcttttttaa aataaatttt gcggagtctg ccttgggcat gcctatacgt
      241 gaaaaggaat ggagtatctg cctgtttaaa ttgctcattt ccacttcgtc aaaatcaagc
      301 agccctaagg tattaacacc ggaaattgcc acgccgtaag ctgccgggca tcctaatcca
      361 cccacacctg cgatcaggac tcttgcattg gataattttt ttaagtcaaa agtgtatgct
      421 tcatttagaa attcaaaaag gttaacagta ctttttgttt gagagaattc catgctcaat
      481 aaatcatagc agagcatatc cccttttatg ctgcctatac caagacaaag cttaacaccc
      541 tctatggtac aaagaactgc agataatgca ttggctaaaa gaaccgggct tttgattttt
      601 gtattattat ttgtgcttgt tttgtactca gcattttctg tttttaccgc gtccataaaa
      661 ttcttaaaaa ggtcttcgtt ttgaaaagat tgaagtgttc ccctccattc accgttaacc
      721 gaaacaatgg ttggtataaa atcttcactt aataattcag ccgccaggct ttttataaaa
      781 ctcaagctgc ccagcactat cctgtaattt tggggacggt tcttttcgtt ctcagaattt
      841 cgattttggc gattttgggg atggctcttt ctggtctcgt tattgagata atcaattttt
      901 gtatcactgt ttaaatcaat tacctcagca aataaggaat ctccatctcc tttgtcttta
      961 aggcaacaaa atattcttcc cgttcccagt gccgcaagat aatatgccag gggtcttaaa
     1021 ctgtctgtat tttcgcaata tacaaagaca gttgaatcaa gaagcttttt ctggccttct
     1081 tcccctattt caggtactat tatgtgtctt ttataatgat ttttctgttc ttccgtcaat
     1141 atcataatat cactcctcgt gtaaatttat agtgacagtt cttttcattt tggtgaggtt
     1201 cttatccttc ttaagaacag aaagaaccat ccctaaaaac taattcagta ccaaagtatc
     1261 tattcccccg tctcttacgg tttcaatact ctttacattt gtataaattt cttcggtatc
     1321 catattaata aagccaaatc caacaaattt ataatacccg attctgtaag aatcattatt
     1381 tttttctaaa accgcagcca ggtcatagcc taaattaaaa taatctccaa acgcaatatc
     1441 aaccaggtcc cagtcagtga aaaatgaacc cagccacttc ttgttcagcc cctctccatc
     1501 ccatgaataa aagaagggtc tgtttcttac atccttataa aatacggtat ccttatgtac
     1561 accgataaat atatccgttt ctccgtcatt atccaaatta caggcatcga ctttccaggg
     1621 ttttatatca gaaaaatcct gtctgtaaat ttctttggct ttcattgagc cattcccttc
     1681 ttcaatgtca tatattatga aatcactgcc ctgttcttca gttgcatgcc tgttgattac
     1741 cagaatttca tcattctcat ctgaatctat attgtatatt ctatggtcaa tcagctcttc
     1801 ataaatgagc ttcttgaata ccttatctgt tttaatatat aaataattgt ctttttcata
     1861 taagtcatat atattttgtt ctattcttat atgacctttt tggtagtatt cgggctgtct
     1921 gtagaatata ccgacagccc ctattaatat aagcaatact attaatattt ttttcatttt
     1981 actcccaata aatctccatg tcatttatgg ttatattgtt ttcttccggc gatatgaagg
     2041 attctgtcca ggcaggttta tcaaccatat tttctgaggg acctgtaatt atgatatcct
     2101 caaaataaaa tgtgtcttca cctataagct tattgcctgc ttcgtcatat atatccattg
     2161 gtacatattt attgctttcc ttgtcttcaa ccatggctac ttttttaact cctaccattg
     2221 tctgccattc ctggtcagta agtcttttgt ccgaaataaa ttcgtaatag ttgaacaagg
     2281 ctccctttgc aagatatgta ttgccgttag tctggcatac cacataaatt tcacagggaa
     2341 gaccgtttcc taattccaaa taagatccct ttggaaacat gttgttaggt gcaattgtgg
     2401 aaacatcagc tattagagct gtagtgtagt cattgcttgt atcaacttcg ttcctcctaa
     2461 ggttcatctg taaaatctga acaaggttgt caattattcc tccgtacctg tagagtgcta
     2521 aattttcttc ctcagtaatg ctttgattcg taagctcttt aattgataca tttaatagtg
     2581 tatcctggat ttcgattatt tgatctaatg tccggccgat gtcgtcctta agcatatttc
     2641 ttgctgccag ctgttctttc gtgttttctg caagccactt gagctttgcg tatacttcca
     2701 catttggctc cacgtaatta tatggtattg tagcttctac tcctcccccc atttcggcgc
     2761 cggactgctt catgtacaat atgctgtcgt gttttaattc ggcatagctg ccaagggctg
     2821 tatgaatatt tttatcggtc catttttcat tcctcatgaa aaagggcatg ccttctctat
     2881 cctcataaga tacagctgaa gatttaatag accacagcca tcccttgtat aagtctgact
     2941 tccattcatc ctctgttatt tctgtctgct tttcccgcat ctcgttcaaa ttttcttcgt
     3001 acttatccca ttcttccttt ggcttgtaat atgtatccaa aatctcttcc gctcttgtac
     3061 tgccaaagga tgccgccaca tcaagccctg agggtaccgg tcttaatatg ggttctataa
     3121 ggttctgcat tacttctgca tcaaaggagt atctttgccc catgaacctg aactgcctgc
     3181 ctgcaggtgt gctgactaaa gtatatttag gttgtatttt tggctcaggc aaaagaagtg
     3241 cttcttccaa aagcttatcc tgataggaat catccttaaa tacattcaaa tccggcgcct
     3301 ggccgtatac ctttgttatt aaatctctca tctcaaaaat tcccaaatca tccgaaatgc
     3361 ctgtataaag tgctgtggtt gtataaatgt tttcgaaatc agtaaaattt ttctcatttt
     3421 ctaaagccag acaagtaatg atcattgatt ttgtcaggct gtccatatcc agcactggat
     3481 cttcttttgt ttcatcaaaa accgggaatc cgcttatgcc gtaccacatc attgttttaa
     3541 aatatttttc gagcttttca tttccggtat aatggcctct cacagtaaac tgtgaataat
     3601 ccaagtcttt tccgaatatg ggcgaattgg caaaatcaaa ggcctcctca ataagcttta
     3661 tctcatcacc agccaaggct gctacttcat aaggaatttc aagaccttca acgcctgcgc
     3721 ctatcaattt tgcaccggtt aaaaaatacg ctgaaacaaa tttcaattcg gcctttaaat
     3781 ctgcatattc aggattattg tatgctttta agctttcgtc aagcatgttt tttgtgagag
     3841 attccagttt gtcatagagg gaagatactt caaggagctt caaggaattg ctgtaataaa
     3901 tatggtatat atgaagggcg gagtccactg ttataaatac aggagattcc ttgtacatgg
     3961 gatattcata tatatggtgc attttcaaat aatcacagct tggtttaagt actacaaaac
     4021 catcttcata aattgactta atctggtttt ttgtaaatcc tgtatattgt cctgaattca
     4081 ccaggttaga caaattttca tctacgtaat atttctcaac aagaggggta acatcaggaa
     4141 cgctgaaagg tacatccaca ttgatttctt ccatattcat gttccatgcc gggggtatat
     4201 tgacttcaat aattccgggt tcttcttcta tcggttccgg ctcttccggt acattattgt
     4261 aagagcagga agcaagtaaa aaaatacaca gcaacaaaac caaaaacttt ttaatattca
     4321 aaataaacct cctgacagcg ggacagcgtg gacgtatctt aactgtctca cttttactaa
     4381 ttttatattt tctatctatg caagtagacg ttatttatta acaaatagtt ccaatactat
     4441 ctttccctga ttgtcacatg aattgtatcc cctggctgtt tattgatttt tgcacgtata
     4501 tcttttctaa tgcctattat gtgacctgga gttcccatcc taactaagct tccttcatac
     4561 ggctcgccat caaaggctgc cagcaccttt actcttcctt ttccgaattc ctttctcaca
     4621 tcatagggga attctatata agcaccgtca atatccggaa ccttcaaaat aaccgcatca
     4681 aattcatata ttttatcatt catattgggg ctccttccag cataatttcc gggatatttc
     4741 tttttgtttt caaatgaaga caataaaccc atcctcttgt cattaacgtt ttaaagatta
     4801 caataccccc gtcccctgtt atccccctgt catttagctt cttcaggcac agagaatccg
     4861 cctaaaaatt cgagcagctc atggggttca tcaattaaat actcagctcc ctgatcttta
     4921 agaaactctt tgtccctgaa tccccaagta acacaaacac agtgcatgtt tgcatttttt
     4981 gcagtcagga tatctacctc agagtctcca atgtaaacag ttctttcttt atccgcactt
     5041 aattcatcaa tggctttata gaccatatca ggtgcaggtt tcctgttaat gttgtctgat
     5101 tgcccaattg ccgttttaat ataatcctca aaatatattt tattcaggct tttaacattc
     5161 ttatcatttt tgttggatac tattgccagc ttgtactttc ttactgacaa ttcctccagc
     5221 aaatccataa taccttcata gggtgatgtt tttatattat tatgatctaa ataatacttt
     5281 ttatattctt cgagtatgct tccaaagttc cgggtactta gtccatccgg caaagcctgc
     5341 tctaaaagcc ttgcagcccc gttacctaca aaacatttta tctctttaca ggttcttttt
     5401 ggatagccgt atttcttcaa aatataatta acaccatctg ctatgtcttc aagtgtgtta
     5461 agcaatgtac cgtccaaatc aaatattatt gtatcaattt ttttcatcct atttcctttc
     5521 tcatcatgaa atattaatta atgattataa aactatggtg ttagttctct atatcggttt
     5581 ttttcattga aaaaagtgat atctgcgtac cctgcatata ttttgccgta atgacttact
     5641 cctgcaggaa tatagtacct gtctccttta acatattcat tcttaactcc gttaataacc
     5701 agttctatct ttccttccag tacaatccca tactggcttt catgactatg ttccggcagg
     5761 tctgcgtctt catcaaattc catgaaaatt atctgatgat ttactccttg ggaaagatat
     5821 gcagacaccc ctcttattgg tatatctgcc agaggcagcc tctctatctc ttctatgaaa
     5881 taccttgaca taaatttcct ccttttataa catactaaaa ttcttttttc ttatatatcg
     5941 acaatgaaat ataaaatgat attaaaaata ttactgctgc aataaagggt gataaatata
     6001 taaatgattt aattgtttcc tcatcaggca ttggaacccc aagtttagat acaatcatca
     6061 atattgcaat tggtgcgaaa aacactaaca tcataagaat tcgccctttt tccaccccgt
     6121 atttaaacaa taatggaaat atgactgaca taactgttat tccggcagat aatgccgcta
     6181 aattaatcaa tatactttca gtagaataca tcttcgaaaa aaccaaatta actaacatag
     6241 ataaaacaaa agctattact ataaaaatca cgcctagcaa atatctgctt actacaatac
     6301 ctactcttga tataggcaag gtaagtgcat atctgtccca tttgcttctt tcatcataag
     6361 acattgctgt tattggtatc attacggcta ccatcgtcat catagagctg aacatggaag
     6421 aattctcaaa gacagcacca taaacaaaat aaaatattac cagtattaag tatacctttg
     6481 cttgtttctt caagtttaat aaatccttta aaataagtcc agtcattata cttgctcctt
     6541 tataataaat atcattatat cttctataga agctttatca actatataat ttccgctaat
     6601 ttcattctta agtattaaag cttcaattcc gaatttattt tttctcattc ctttaacaat
     6661 gcttttatct atgttttcga actcttctac cgagcatttt aaaataccat aatcgtataa
     6721 taaatcatcc ttcgcttctg aaaatattat tttccctttg tgaataaatg taatataatc
     6781 gcagattttt tccaaatcac ttataatgtg agaggaaaca aatatggaat tggattcatc
     6841 ctgtataaac tctaaaaacg tatcgagaat ttcttccctt ataataggat ccaaaccgct
     6901 tgttgcttca tccaaaatca gaatttttga atcatgggac aatgaaacgg ctattgaaag
     6961 tttcattttc attccccttg aataatcctt aacactttta ttatgtgata ttgaaaattt
     7021 atcaagaaag ctaaaaaact tattctcatc ccaggtttta tatatttttt ttaatatatg
     7081 attaatttca gcagcattta aattctcagg aaaacttgat tcatccagta caactccaat
     7141 attctcctta atggcattca tttctgtcct gttgtctttg cctaatattg taattttccc
     7201 atcatcacgg ttgattaaat ctaatataag ttttatagcg gtagtttttc cggcaccgtt
     7261 ttctccaata aaacccatta tacatccctt gggtagagca atattaatat ggtctaattt
     7321 aaaattctta tattcctttg ttacattttc caaccttaat gcatattcca ttttattctc
     7381 cttcataaat caatttcatc atttcaatca attcatttaa ctctataccg caattcccgg
     7441 acagcttgac tatttcctgc atatggtttt ctattgactt cagcagctgc tcttttatca
     7501 aacctatatt cttgcctgca acaaagctcc ctttgccggt aaatgaaaca ataaaccctt
     7561 ctctttcgag ttcatcatat gcccgctttg ttgtaatcac actgattctc agctcccttg
     7621 ccagaagcct cattgaaggc agtgcttctc cttcattcaa ttcacctgta ataatgagag
     7681 attttatctg agaaacaatc tgttcataaa taggttggct gcttgaattg cttattatta
     7741 tattcatttt caactccatc aacattgtat atatccatta tatacattat atcaactttg
     7801 tcaatatttt tttggttaaa tgaatgttga tttctataag acgacttggt acaatatgaa
     7861 cacaatgtac cgggaggcgg tattatgtca agatagccca tggtatagaa tcaacaaaga
     7921 aatataaagc aaagggataa tattatgaag atatcaaaac aagaatttga agaagaaaag
     7981 gaatcatccc caaaatttaa atatacggat gtaaactcaa aattttttga caagtggatt
     8041 gatgaaggat gggaatgggg taagcctgtc agccatgaaa tatatgaaaa ggcaaaaaat
     8101 aatgactggt ttgtcttgct aacaccaact aagccggtac cgaaggaatg gttttgtgaa
     8161 atgaaagatt cgaagattct aggacttgct tccggaggcg gccagcagat gccgattttc
     8221 actgcactgg gtgcaaaatg tactgtactg gattactctg agaaacaaat tgcaagtgaa
     8281 atagaggttg ctgaaagaga aaattatgaa ataaaaactg ttagggctga tatgacaaaa
     8341 cccctgccat ttgaggatga aacttttgat ttgatatttc atcctgtatc taattgctat
     8401 atcgaagatg ttcttcctgt ttggaaagaa tgctacagag tattgaaaaa aggcggaata
     8461 ttattggctg gattagataa cgggattaat tttattttcg atgatgatga aacaaaaccg
     8521 gtatgcaaac tgccctttaa tccactgaag gatgaacggc tttatcagtt ttcaatagaa
     8581 aataactggg gaattcaatt ttctcattcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnt ttttcagcct tattgtataa aacatttatt acatccgata cttcaaacag
     8761 cttgtaataa agtgctatat ttatttcatc aagaattacc aggtcatatt ctccgcctgc
     8821 taaaatctct ccgcattcat ctaatccctt ctttgctgct tctttgtcag catcttcagg
     8881 tttttcatat atgaagcagc ctctgccaaa ttgttttatt tggatattat ccaagtaatt
     8941 ttcaatcctt gtttcattat atttcatgtt cttaacaaat tgaccgatga aaactttttt
     9001 gcctgacatg gctgctctta aggcaagccc gaaggcggca gttgttttcc ctttcccgtt
     9061 gccagtatat atttgaacat atcccttgtc catatttgtc tcctcatttt tacctaatag
     9121 tattgccttt aaaagcagta gtaatatata ctcccgcaaa caccaggagt ccccctgtta
     9181 tttgtaccca tgttatttcc tgccccattg caaagcttat aaatgcagta aagacagggt
     9241 ttaaattcat gaatatacct gctttaacag caccgatttc tctgactcct atattccaga
     9301 aaacaaagga taacattgac ggaaacagaa taatatatat gacggcagta aatgccaggg
     9361 ggctgatatt taagtaatca actccattgt tcattgcaaa tggaagaagt attattgccg
     9421 caataagacc ggaaacacct gtagatgtaa tgggaggaac agttgtgact tttttcccta
     9481 ttaacgagta tactgtccac gaaattacag caactattat aagaaaatct ccagtattat
     9541 atttcattgt aaatgcctgc atcaaatttc ccttggttaa aacaatcaat acacccagga
     9601 atgaaataac aagcccgaca atgtgatgtt ttgtgatttt ttcttttaaa tataaggctg
     9661 aaacaacggc aataaaaccg ggattgaagg aattgaccag agcggcatta aggggcgatg
     9721 tataataaaa cgcatagtac aagagcacat tatagcctat tatgccaaac aatgatagcc
     9781 ccaccatttt aggccactgt ttgtaaacct ctttccaact gggtttttcg attttgtgcc
     9841 caaccgctat cagtataaca gatgcaaaca accatcttat gaatgttaac cccaccggtg
     9901 tcaattctgt aacaaccagg gagccgaaaa tataattacc tgcccaaaat aaaacacata
     9961 acaccaaaag tccccatact ttatattttt tcaacactaa ctcctctaaa ataaattctg
    10021 attttgttta ttctatatta acttcatttc attcatatca ttaagttaat ttcaacatat
    10081 taataagtct tgcataattt taccatatgt tacaatatat ttcaattgtt tacatttttc
    10141 ttgtaataat ctgcttctat tacacaaacg gataacaaac accaaaaaaa ttatttattg
    10201 ttgagaataa aaagaaccgt ccccagaatg aaaagaaccg tccccgaaaa aaaattattt
    10261 ttcacgatta tatttaaaat atatcactaa gtgcatggct gtaggaatgt ttgctattat
    10321 gaaaataatg aatagtatta gctttgatga ggtcatattc atagagacta tgactataaa
    10381 aattgaaaca acaaaaaata atatagcaag catgggacca aaatatttgg ttttcttttt
    10441 tgctacatat attaacacta cacataaaac agctattaaa atcaacaaaa aaacttcttt
    10501 gctcattatt ttttccgccc cttatttatt tattatttga tatgcttttt ttaaatcaat
    10561 caacagtccg ataaaaaata caatgcacaa gaattaagct aacgacaaaa aagcgatttg
    10621 cagccagatt ttgaatttta ttataaggct attcctgttc attttccggt tttaaaagtt
    10681 cttctgccaa ttcagccatt tcttcaggaa cataaatatc tattccatac atgttcacac
    10741 ctgtatatat ttccatcagt tcgccggtgc ccttagtctt ttttaaagca ggtattccgt
    10801 atgattttaa ttttgatata attatttctg cttccatggt attaaggttt gaaagcaata
    10861 atttttcatc ggacataatt cttccgcctt cctaatagcc aaatattcta tcatcaacaa
    10921 gtatatcaaa taatattttt tctatcaata acaaaaaacc ctatacaatt tactaaaata
    10981 ttcccggtat aagcctggct atttgaaatc ccaggtaaaa ggatactaca ttagctacaa
    11041 atgcatacaa tactgaatag cttttcctgg tcagcctgcc ggtgccgtcc ttggtgagaa
    11101 agctggcata cacgccggct tcaattaaaa atacaacaat ttcagctaaa atatagctta
    11161 gtaaaaatgc cagccctcca taatagtaat tgaccgtatt aagcataata ttaagaattg
    11221 tctgggttgc aatattggtt attaaaataa tacggagctg tttctttgcc ctatagccaa
    11281 acagaagggc aacaagaatt tcaattgcta tggttgcaat aattctaaga aaaagggaaa
    11341 taatctccct ggtgaaatta taactttttt ctgctgttat tccctcatgc actttcaatg
    11401 gagtaagttc catattttct gcatcaactg tataataaga agcaaaggca taccgctcat
    11461 agacatcact tactgcaaag ctgtccaatt ccggaaaata cagtaagatt ttaaactttt
    11521 ggggagggta ataagtccat ttgaattctt gagtttctgt acagtcgcta aagtactgta
    11581 aaaaataata accgtcttca tcctggtaat caacgaattt attccagatt tcataatctt
    11641 cttcatcctc tgaatatctg cggtctcctt cgaatctccc taccgcactg tagggacctg
    11701 ttgatgatgt ttcagacaac aaggttgtat aatatgtttt tccttgaaga ccttcaaaat
    11761 ttactatgac agaaggttta ggcccgacat ctccatatgc caaaggcgga ataaaagcaa
    11821 acattgccgc aatacacaat aaaacaataa gaaatttctt tttcatatta attccttcct
    11881 cgacggtaac aattcccact aaagcttagt tttacgtcat agcaatttaa ccaaggatga
    11941 attctctaaa ttcatccacc gttgaaaagt aataatctga attatttctc atgtaatctt
    12001 ctatttcagg aactccatga gaccatcctg ccgctgcaaa ttcaacactg cagcttcttg
    12061 ccatggtaag tcccggcttt aaatcatcca aaacaagcat ttctgtattt ttcaaattaa
    12121 agcttttcat agtttcaaca ataggataag ggtttggttt tctcttgctc tcctccatat
    12181 cccacccaaa tatcaaatcc ggaattatgc cgtcgcaatg caaggtataa tctctgatga
    12241 tatggcggct ttccgaatga gtcgcaacac atattatccc ccctgaactt ttatagtctt
    12301 tgattaattc agggaaaccg ggataaaagt ccggtgaatt ttcattaacg taatccttcc
    12361 atattttata ctggtattcc tgctctgttt cattaaattt cattatatct gtgcaaagct
    12421 ctacaaatcc cggagcaaaa caatagttaa caaaatcatt aaggcttagc tcaactttag
    12481 gtctaaggga ctttaaagct tctacaaaag aaggatagtg tattgcaggc gtacttttaa
    12541 ccactgtatc atcatggtct aatattaaac atttgtattt cattaactgc tccttnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaggat taggctgaac aggacctgtt
    12721 attatatcta tgcccttttc cttaataaag ttaatctttt catccagtga ttcaacttta
    12781 aaacccaatg taacccggtt tcctgcatcc agttttttta acttctcact gcatatgagt
    12841 tcaacctttg tttccccctc acctaaaaag gatatttcca tccccggtcc tgcctggaat
    12901 ctgtcagcta atttaagacc cactatatct tcataaaact tcaaggattc atccatatta
    12961 tctaccagca gcgtagtcca taaaaatttc ataattcccc tccaaatcat atattttata
    13021 atattttaac ataattttaa tagtatttca ttaaaaatag attataatta gttaatatat
    13081 gaaagatgcc ctaaccctgt ttgctgatct aataatttaa ataacatatt ttttaataat
    13141 tcatatatat atattgatat caaatcaaaa aataataata aaggggaaat tatttatgac
    13201 aaggtattca gcggaaagca acctacataa taaaactatg acactgacag gcaatggaga
    13261 gatggatata gctcctgaca ctgtattaat tcgtttaggt gttcagacta caggagaaaa
    13321 tctttctcaa atacaggcag agaacgcaag aataagccag tctattattc agacacttca
    13381 aaggatggga gttacagaca taaagacagc tcagtattct atcgacaagg tttatgatta
    13441 tgttgaagga aggcagattg acagaggctt ttctgtcagg aatattttag aaataagaac
    13501 gaataatctg gaagctgcag gctctattat cgatgcttca gtgaatgcgg gctcgaatgt
    13561 tgtggatttg ataacctttg atgtatcaaa cagagaatac tattaccggc aggctctgaa
    13621 tatggcaatc ggtaatgcaa tacaaaaagc taagtcaata gccgtgaatt taggaatatc
    13681 tacaactcct gttcctgtta atattgtgga aaacacggca atgccttttc ctgttcagcg
    13741 ggaatttgcc gctactccca taatgccggg aaccataaag gtggaagcca atgtaactgt
    13801 ggattttgtg tattaagaat ccacccttaa tatgtctttt atcatttcag gacgaatttc
    13861 ccagatattt gcctgaattc tgaatatatc ccatttatca atttcaaaaa ctctagccca
    13921 gctgtccatc accctttttc tttcatgagg ataaaaatgg gctgtttttt cgtcaataaa
    13981 tgaataagaa tttttatagc ctttgctttc tatatccttc aaatattctt cagcatcctt
    14041 ttcatccttg ggaacatagt gatggtttaa tacataatcc catttcatac cgtcaaaata
    14101 cactatgtcg gatttatcaa cttccaaaac atacacaatt tcatataagg taggtctcag
    14161 catatattcc tcactgatgg agcaccatat gggaaattcc acgccctccg gttttggaac
    14221 tcttttttct gcctcatcca caaaccattt atacaaataa ataatatggt cggatataag
    14281 gtcgaatttt tcctccaaat gccccctggt tattctgatt acgccgtctt tttccaaatt
    14341 atcatagctt cttttatcct gcctggtcca taaagttacc ttatccataa atgctcctta
    14401 ctttattgat tttattcatt atatcaaata tcactatttt gacaagaaaa aaagcatgtt
    14461 gttttttcca gcatgccctt agcttatatt caattaaaat aatcattcat aattaccgac
    14521 tcttatatca tctatcagcc acttgtttcc aacttttctt acaatcagtt caacaggtct
    14581 gtacacggca gccccacctc cggcttctga gcttgtaacc tcaacaattc tgccctttac
    14641 agagaattca ccctcagaca gccttacaac ttctgtgatt tcaatacgtt caggccaggg
    14701 gcttgatgta gttctcccgg gagccctcct tggatgagca agccagcttt gcataagctc
    14761 atcagttaca taaggactgt aattggcttc tattgcttct ataaccaagt ctctgggagc
    14821 caaaagcgac acatttttaa gagtgcttcc aaaatcctca accaatacta ttacatcttt
    14881 actttcatct tttgaattat cgttattgta aatggaataa ataataaatg cagccatcaa
    14941 aaaaactata actgcaatga taatataaag ctgatttctt cttataaaca taaatatccc
    15001 ccctttttat gttatacata aagggatgga aaaattacta ttattttaag acaagatttt
    15061 cggaaggggc ttgttcttta gcagttatta tattttctgc aattataca
//
LOCUS       DAOPUT010000039        15047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_4035_length_15047_cov_20.494064, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000039 DAOPUT010000000
VERSION     DAOPUT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4035_length_15047_cov_20.494064"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKK75_04930"
     CDS             <1..303
                     /locus_tag="PKK75_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ82974.1"
                     /translation="LLMATMNKPELLLLDEHTAALDPKTSRLVMEKTQELIKTQNITT
                     MMISHNVRDAVKYSDRIIMLDKGEIILDVRNGLITEKELLNIYNSRNYGSPLTEAV"
     gene            471..1160
                     /locus_tag="PKK75_04935"
     CDS             471..1160
                     /locus_tag="PKK75_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ82975.1"
                     /translation="MEDIIKQIKNNPLFANMREDEIQHVLRCGDAAVANYTDNQILFE
                     KDHRVEKLGIIIEGVLNLVSWKYNGSRAIVTTLEKNDLFGEALIYASAENSPYDLISS
                     GTSKVLLIPYRIFSNMCSKSCSYHNQLINNMLTILSNKIVMLNNKMRILNAETLKGRI
                     AAYLLSLQERANTMIFDMPMKRQELADFLNVKRPSLSRELSNMQKDKIIEVYRSSVKI
                     LDMDKLQELAD"
     gene            1239..2324
                     /locus_tag="PKK75_04940"
     CDS             1239..2324
                     /locus_tag="PKK75_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ82976.1"
                     /translation="MRIKFIHTSDLHLGKKFDIKNFSLKERKKRRQELMDSFDEIIWA
                     AKINEVKYLFIAGDLTEGEYISYKVLNEISSKLRSIPGTRAIISCGQSDPCNISNMYE
                     YIQWPHNVHLIKNTETVEKLNFPDDNLCIYSISWDNHEENRKTQSIYDISVDDSKINI
                     LMLHCHIDSTDRLPVNIDVIRNKFDYCPLGGRHNFEQITDNAVYCGAPEPLSFEEEGD
                     HGIITGTLEKKNVQYTLLPIAKRKFVNRRIELNYSHSYNKILDLIKFSGDTFSNIKDY
                     VHIELTGGVNTDISMDEIENEAKQFFYYIEFEDNYIYETENRNYEGNEYNIVESYKLQ
                     FDNNDDKLQQQAFKLGLEVLRKEKVVK"
     gene            2326..4998
                     /locus_tag="PKK75_04945"
     CDS             2326..4998
                     /locus_tag="PKK75_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ82977.1"
                     /translation="MYISRLHLRAFGKFISRKIYFGSKLNIVFGENESGKSTIHNFIE
                     YLMYGFDNISGYNKYKPWNSPLFKGSAEIGDEEKKYLVSRDFLLDTVQVFNKTNEINQ
                     NEYPMEIIEVPGEYFFNINRGAFNNTVSIKQLGNKTDEELAEELKNKITNLSRTRDED
                     ISIDRIMQTLNRIKEEAGSENNEKTLLGQYSLRLNELANARENSLNSGRQVMFLAMEK
                     KKLQSKIHELNAKIEEKNKEISDYELSLEKQKFLRALPVKKEIDNIDNELKSFEKDVT
                     NYSKEDYKEAAETEVSLASMKNDRQRMYEEKEECIAELEENEFDLANSIPDNFDIDKI
                     NMDYGIYKANKEKLDTLKAKTESGRETIDTINIDEINKFIDDYREAEELTRKIEINNM
                     LGDSKNYDLMRNFARKQSLLSLLFGLLGTAFTGAAAASLYAAYYYNNISFYYGGTGFL
                     IGIIFYVFSWRKRNLALGCKKEIESMECEFADYALSNSQMNEQKNEIIKRNNCEDFYN
                     MADVFQKKNTEKSLYDEKAKLLNYDDNKYSEISEANRKLEINLNNILSSLNLPLSQDN
                     ILEVNEAYKRKDSIKLQVLNLKNKIEQINLDMAKLDKEINFEERRLEMILRANGVEDV
                     ESFKNAVSINEKHKVLLNRREYSQNVLENIIGGSDYFELKQRTLNVPDEVKEINRQDI
                     QLDIFRLNEEKTNLLKTIEDIHREIEDIEDSTRSLAEIEEEVSFYEEKKAFFKNKVKI
                     AEIAAEKIAGISDSIKGDFMPLLRSSISENFSYLTGDRYKKVDIDENMNITVMSEEEK
                     DRKIALENLSGGTLDQLYLSLRIALSNILSGNQNIPLILDDSFIQYDSKRLRKSLEML
                     SRESERRQVILFTCQEREEDLAKAMNIKFNYFRL"
     gene            5007..5696
                     /locus_tag="PKK75_04950"
     CDS             5007..5696
                     /locus_tag="PKK75_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ82978.1"
                     /translation="MKIFAIADLHLDNKNEKPMDVFGDNWENHEEKIFENWEETVGKD
                     DLVLIPGDISWSLKLDGALEDLLKIDKLPGTKIIGKGNHDYWWSTASKLEGLGLKTIK
                     FLKNNFFKFNDVLVCGTRGWDTMEDHDSEVDNEKIFLRELNRFKISLQSGRKEKGKKI
                     IMLHYPPFSRDGAANEFFSLMKDYDANILIYGHLHGEEGHKNVKEGLIEGIMVHCVSS
                     DYLDFKLKRIV"
     gene            5865..7052
                     /locus_tag="PKK75_04955"
     CDS             5865..7052
                     /locus_tag="PKK75_04955"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNZ82979.1"
                     /translation="MFKAKKYYSDILDEIKASGLWKNERIITTPQGSIISTTKADGVL
                     NMCANNYLGLADNKEIIQAAKDAYDRWGFGLSSVRFICGTQEIHKELEKKLSKFLKTE
                     DTILYSSCFDANGGLFETITTENDAIISDELNHASIIDGVRLSKAKRYRYNNNDMSSL
                     EEQLIQADKDGVNIKLIATDGVFSMDGIIANLEGICDLADKYNALVMVDDSHAVGFVG
                     KTGRGTHEYRNVMGRVDIITGTLGKAMGGASGGYTSGRKEIIDLLRQRSRPYLFSNTL
                     APAIAGASLKVLEMLTESTKYRDKLEENTKYFRQEIKKIGLNIIDSEHPIVPIMLGDA
                     VLSQKMAEKLLEKGVYVVGFYFPVVPKGKARIRTQISAAHSKEDLDFALKAFKEVKEE
                     LGI"
     gene            7289..8233
                     /locus_tag="PKK75_04960"
     CDS             7289..8233
                     /locus_tag="PKK75_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNZ82980.1"
                     /translation="MSDIVLKEVKKTSIGGQALIEGIMMKGPSKTATAVRKPDGEIAV
                     KESSIAPIFKNKFFKLPIIRGSFVLIEALVGGVKELMYSAEFFGEDYEEDALDKFLKK
                     VFKEKADTAIIYASVVIALVFSVVIFILGPTFLTNLLKAIIRNSLLLNLVEGIVRVLL
                     FVGYVYMVSKLEEIKRVFMYHGAEHKTIYCYEHGEELTVDNVRKYSTLHPRCGTSFMI
                     NVMLISIIVFSFFGWPNPFLRLLIRLAMLPVIAGLSYELNRYVGRCSSDNIFTKIITY
                     PGFLIQKITTKEPDDLMIEVAITAMNKVIPREGEDDRW"
     gene            8227..9078
                     /gene="prmC"
                     /locus_tag="PKK75_04965"
     CDS             8227..9078
                     /gene="prmC"
                     /locus_tag="PKK75_04965"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNZ82981.1"
                     /translation="MVTVEELLKAGTDIIKPREFNSPGLDSELILCYLLQKDRIYLHL
                     NRKTEISEYIKDKFYELAKKRNEGYPLQYITNSQEFMGLDFFVQEGVLIPRPDTEILV
                     EAVIETAKARYNDRIKILDLGTGSGAIAVSLAYYLENSFVTAIDISKKAVETTNINIK
                     KHKLNNIKVIEGDIFDDIADEKFHIVVSNPPYIERDAISSLQTEVSVYEPKIALDGGT
                     DGLDYYRRIVELFKNIHEKNGVLAVEIGWSQRISVEEIFMNAKLFKEIKSCKDLSGRD
                     RVVCGFL"
     gene            9158..10228
                     /gene="prfA"
                     /locus_tag="PKK75_04970"
     CDS             9158..10228
                     /gene="prfA"
                     /locus_tag="PKK75_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNZ82982.1"
                     /translation="MLEKLEALKDKYIELGEKLLDPDTLSDSQKLQKLMKEQAQLEPV
                     VLKYNEYTEVANQLEESKEMLREKLDDDFKEMVKEEIKELTAREEELENDIKILLLPT
                     DPNDDKDVIVEIRAGVGGDEAGLFAGDLLRMYLRYAERKGWKTEFITVNDQGIGGYKE
                     VIFSIKGKGAYSKLKYESGVHRVQRVPDTESSGRIHTSSATVAVLPEVDDIDIEINPN
                     DVRVDVFRSSGNGGQCVNTTDSAVRLTHIPTGIVISCQDEKSQLKNKDKAFKVLKARL
                     YDKYLQEQNDEMAQARKSQVGSGDRSERIRTYNFPQGRVSDHRIGLTLYKLDAFLDGD
                     ITEMLEALITSDQAEKLKNISE"
     gene            10275..10520
                     /locus_tag="PKK75_04975"
     CDS             10275..10520
                     /locus_tag="PKK75_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNZ82983.1"
                     /translation="MRIDKFLKNARIIKRRTIAKEACEQGRVLINGKTAKPGTEVETG
                     DEIEIIFGIKPMKIRAEKLLEHTAKDEYKEMYSVINV"
     gene            10619..11155
                     /locus_tag="PKK75_04980"
     CDS             10619..11155
                     /locus_tag="PKK75_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNZ82984.1"
                     /translation="MELKGSKTEQNLMTAFAGEAQAHVKYTYYQSQANKDGYVQISKY
                     FEETARNEKEHAKLWFKLLHDGIAHTTVNLKDAADGENYEWTQMYADFAKVAKEEGFN
                     KIAYLFEGVGAIEKNHEERYLALLNNIETGKVFKREGEIVWQCNNCGHIHVGAEAPKV
                     CPVCDHPQAHFQIYTKPY"
     gene            11429..12601
                     /locus_tag="PKK75_04985"
     CDS             11429..12601
                     /locus_tag="PKK75_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNZ82985.1"
                     /translation="MDSNTHEAVKLTQELIRIPSHKYVENRESNIAKFIYDYCSKKGL
                     EVEFQEVEGLRRNVIARLKGNGTGKNLIFNGHTDTVPPYEMTIEPFGGEIKDGYVLGR
                     GANDMKGAVACMITAMVNIKNKGIVLGGDIILTAAVGEEEKSDGTEYVVKSGITADGA
                     LVGEPANYGYALGHRGLEWLEIRIEGRTAHGGIPELGINAISKAARLIRKIEEDLMPK
                     LKERQNEWMGPSVMNFGLIKGGTQPSSVADSCIIQIDRRYLPEENVESVIKEYQDIID
                     ELKQEDPDFKAEIIRMDSNLMEEFDHAPLIAQPDSDIARTVYNVLKDFNKKEPNIEKR
                     RGWTDAGVLSTYGKIPTVVTGPGDLKYSHARDEKIPVQDLVNYVEIYTRIAEKFCK"
     gene            12615..13982
                     /locus_tag="PKK75_04990"
     CDS             12615..13982
                     /locus_tag="PKK75_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ82986.1"
                     /translation="MLDIKIINGKIIDVENKRIVEGDIGIKDGKITDIGSVPDEGKVE
                     IDADEKYVSPGFIDIHMHEEDFTLTKKEEYDISDTMLNMGVTTCAVGNCGNNRQSIKE
                     LYEFINEKGNPVNYLTYIGHNFLRVEAGNTDIYKRSSREQIEKMQKWVADAVDFGAIG
                     VSYGLEYCPGIDTEEAIAITKEIQGRDDLLLAAHYRKDAIHALDSINEMAYIGKETKI
                     PFQISHLSSCSAFGNMTEALNLIQEIHDSGTNIMVDSYPYDAFSTYIGSAVFDEGCFE
                     TWGKSYEAIMLTEEPYKGIYCDRELFEKVRKEYPKMLVVANVMNEEEIIEALNHHLVM
                     AASDGIYRNHSGHPRGAGTFPRIIGRFVRDMKVMEFFDAVYKMTYMPAKRLKLNKKGI
                     LKAGYDADITIFNYDTIIDKATFQEPQLRPEGIDYVIVDGKVAIEKGITVNNTLGKFY
                     KRGGA"
     gene            14064..14255
                     /locus_tag="PKK75_04995"
     CDS             14064..14255
                     /locus_tag="PKK75_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82987.1"
                     /translation="MEAKKKKFKVPHTYVILFAMIIIMALLTYVIPAGQYQKVEGPGG
                     RMIVDPSSYASVEASPAMP"
     gene            14206..>15047
                     /locus_tag="PKK75_05000"
     CDS             14206..>15047
                     /locus_tag="PKK75_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HNZ82988.1"
                     /translation="MTQALMQVWKQARQCLDILKAFPKGLAAAQSIVFFIFIVGGSFN
                     IVNQTGAIEAGISKVALSLKGKEKLMIPIIVFVFSLGGSTIGMAEEAIVFVPIGVALA
                     RALGYDAVTGMAMVSLGAAAGFTSGFMNPFTVGVAQGIAELPLFSGIGLRIIIWICTL
                     ILVIGYIYRYANKVKADVKNSYVYELEMQEKDMAIDLSNVRKMTKRDTAVLLIFVVGM
                     AILIFGVFKYGWYITEIAAIFLAIGIFSGIAAAMPIDDIAKHFILGAKDMTMGALVVG
                     LARG"
BASE COUNT         5515 a         2317 c         3239 g         3976 t
ORIGIN      
        1 ttgttaatgg caacaatgaa caaacctgag cttcttttgc tggatgagca cacagcagcc
       61 ttagatccga aaacatccag gcttgtgatg gaaaaaactc aggaactgat caaaactcaa
      121 aatattacta ctatgatgat atcccataat gtaagggatg cagtcaaata ttcagaccgc
      181 ataatcatgc ttgacaaggg agaaatcata cttgatgtaa gaaacgggct tataacagaa
      241 aaggaactgt taaatattta caattcaaga aactatggca gcccgctgac agaagcagta
      301 tagcaataaa taaaataatt ttcttattcg gtattttatt ttatacggac taacagtata
      361 taaacacctg aaggaatgga gaatttaaag attttccatc cttttttatt tttttgacac
      421 ctacataacg gtattgtata attaatatgc cgcttgtagg aggtgtttgt atggaggata
      481 ttataaagca aattaaaaac aatccgttgt ttgccaatat gagagaagat gaaatacaac
      541 atgttctcag gtgcggcgat gcagctgttg caaattacac tgacaatcaa atcttgtttg
      601 aaaaggatca cagggttgag aagcttggaa ttataattga aggtgtttta aatctagtat
      661 cctggaagta caacggcagc agagcaattg taacgacact ggagaaaaac gatttatttg
      721 gagaagcctt gatttatgct tcagcagaaa attcccccta cgacctgatc agctccggca
      781 ccagcaaggt actgctgatt ccatacagaa ttttttccaa catgtgttca aaaagctgca
      841 gctatcataa tcagcttatc aacaatatgc ttactatcct gtcaaataaa attgttatgc
      901 tcaataataa gatgaggatt ctaaatgctg aaacattgaa gggcagaatt gcggcatacc
      961 ttttatctct gcaagaaaga gcaaatacca tgattttcga catgcccatg aagaggcagg
     1021 agcttgcaga ctttttaaat gttaagagac catccttgtc aagagagctt tcaaacatgc
     1081 aaaaggacaa aattattgaa gtctaccggt catccgttaa aattctggac atggataaat
     1141 tgcaggaatt agcagactaa aattttatct attaatattg aaggcatgca ctttctgtga
     1201 tacaatatta tatacttaca caaatcgagg gagcggatat gagaattaaa tttatacata
     1261 catctgacct gcatttagga aaaaaattcg atataaaaaa cttctcttta aaagaaagaa
     1321 aaaaacgaag acaggaattg atggattcct ttgatgaaat aatctgggca gctaagataa
     1381 atgaggtaaa atatttgttt attgcagggg accttacaga gggagaatac ataagctata
     1441 aagtattaaa tgaaatttcc tcaaaattaa ggtctattcc gggaaccaga gcaatcatct
     1501 cctgcggtca atcagatccc tgcaatatca gcaatatgta tgaatacatt caatggcctc
     1561 ataatgtaca tttgataaag aataccgaaa ctgtcgagaa actgaatttc cccgatgata
     1621 atctgtgtat ctattcaata agctgggaca accatgagga aaatagaaaa acccagtcta
     1681 tttatgacat ttccgttgac gattcaaaaa ttaatattct tatgctgcac tgccatattg
     1741 actcaactga cagactgcct gttaatattg atgtaataag aaacaaattt gactactgcc
     1801 ccttgggagg caggcataat tttgagcaga taactgataa tgcagtatat tgcggcgctc
     1861 ctgagcctct atcatttgaa gaagaaggag accatggaat cataacgggg accctggaga
     1921 aaaaaaatgt acaatacacg ctcttgccca ttgccaaaag gaaatttgta aacaggcgga
     1981 ttgagctcaa ttattcacac agctataata aaatacttga tttgattaaa ttttccggag
     2041 atactttttc caatatcaag gattatgtcc acatagagct tacaggtgga gtaaatacgg
     2101 atatttcaat ggatgaaatt gaaaatgaag caaaacagtt tttttattac attgaatttg
     2161 aagacaacta tatttacgaa actgaaaaca gaaattatga aggcaatgaa tacaatatag
     2221 ttgaaagcta taaacttcaa tttgacaata atgacgataa attgcagcag caggcattta
     2281 agctgggact tgaagttctt agaaaagaaa aggtggtaaa ataaaatgta cataagcaga
     2341 ctgcatttga gagcctttgg gaaatttatc agcaggaaaa tctattttgg cagcaagtta
     2401 aatattgttt ttggggaaaa tgaatcggga aaatccacca tacataattt tattgagtac
     2461 cttatgtatg gatttgataa tatatccgga tataacaagt ataaaccatg gaacagccct
     2521 ttgttcaaag gaagcgctga aataggggat gaagagaaaa aatatcttgt ttcaagagat
     2581 tttcttttgg atactgttca ggttttcaat aaaacaaatg aaataaatca aaacgaatat
     2641 cccatggaga ttattgaagt accgggagag tattttttca atatcaaccg tggagccttc
     2701 aacaatacgg taagcataaa acagcttgga aacaaaactg atgaggaatt ggctgaagag
     2761 cttaaaaata agataacaaa tttgagccga acaagagacg aagatatttc tattgataga
     2821 atcatgcaga ccctaaatag aataaaggaa gaggcaggaa gcgaaaataa tgagaaaacg
     2881 cttttagggc agtattcact gagactgaat gaacttgcaa acgccaggga gaactccttg
     2941 aattccggaa gacaggttat gtttttggcg atggaaaaga aaaagcttca aagtaaaatt
     3001 catgagctca atgctaaaat cgaagaaaaa aacaaggaaa tttctgatta tgagctttca
     3061 ttggaaaaac agaaatttct tagagccctg cctgtaaaaa aggaaattga taatattgac
     3121 aatgaattga aatcctttga aaaggatgtt acaaattatt caaaagagga ttataaggaa
     3181 gcagcagaaa cagaagtaag cctggcttca atgaaaaatg acaggcagag aatgtatgaa
     3241 gaaaaagaag aatgcattgc ggaactggaa gaaaatgaat ttgatttagc caactctatt
     3301 cccgataatt ttgatattga caagataaat atggattatg gcatatataa ggcaaataag
     3361 gaaaagcttg atacccttaa agcaaagacg gaatcaggca gggaaacaat agatacgata
     3421 aatatagatg aaattaacaa gtttatagac gactacagag aagctgaaga gctgacccgt
     3481 aaaattgaaa taaacaatat gttaggcgac agcaaaaatt atgaccttat gaggaatttt
     3541 gccagaaaac agagcctttt gagcctgctt tttggacttt tgggaacagc cttcacaggg
     3601 gctgcggcag cctcactata cgcagcttat tattataaca acataagctt ttattacgga
     3661 ggaacaggtt ttttaatcgg tataatattt tatgtttttt catggaggaa aagaaacctt
     3721 gcattgggct gcaaaaagga aattgaaagc atggaatgcg aatttgccga ctatgcattg
     3781 tcaaacagcc agatgaatga acagaaaaat gaaatcataa aaagaaataa ttgtgaagat
     3841 ttttataata tggctgatgt ttttcaaaag aaaaacacgg agaaaagcct ttatgatgaa
     3901 aaagcaaagc ttcttaatta tgatgacaac aaatatagtg aaatttcaga agcgaacagg
     3961 aaattagaaa taaacctgaa taatatttta agcagtttaa acctgccctt gtcccaagat
     4021 aatatccttg aagttaatga agcctataaa aggaaagaca gcataaaact tcaagtattg
     4081 aatctgaaaa ataaaataga gcaaataaac ctggatatgg ccaagcttga caaggaaatc
     4141 aattttgagg aaagacggct ggaaatgata ctcagggcaa acggtgttga ggatgtggag
     4201 tcatttaaga atgcggtaag tataaacgag aagcacaagg tgctgttaaa ccgcagagaa
     4261 tattcccaaa acgttttgga aaatataatc gggggctctg actattttga acttaaacaa
     4321 agaaccttaa atgttcctga tgaagttaaa gaaatcaaca ggcaggatat tcagcttgat
     4381 attttcaggt taaatgaaga aaaaacgaac ctgcttaaaa ccattgagga tattcatagg
     4441 gaaatcgaag atattgaaga cagtaccaga agcctggcgg aaattgaaga agaagtcagc
     4501 ttctatgaag aaaaaaaggc cttctttaaa aacaaggtaa agattgccga aattgcagca
     4561 gaaaaaatag ccgggatctc agattcaata aagggtgatt tcatgcccct tcttcgaagt
     4621 tccataagcg aaaatttttc atatttaacg ggagacaggt acaagaaagt tgatattgat
     4681 gaaaatatga atattacagt tatgtctgag gaagaaaagg acagaaaaat cgctcttgag
     4741 aatttaagcg gaggcacgct ggaccagctt tacctttccc tccgtatagc tttaagcaat
     4801 attttaagcg gcaatcagaa cataccgctg atattggatg acagctttat tcaatatgat
     4861 tcgaagagac tgagaaagtc tttggaaatg ctgtcaaggg aaagcgagag gcgtcaggtt
     4921 attcttttca cctgtcagga aagggaagaa gaccttgcaa aagccatgaa catcaagttc
     4981 aattatttta gattgtaagg aagattatga aaatatttgc tatagccgat ttgcatctgg
     5041 acaataagaa tgaaaagcca atggatgttt ttggcgacaa ctgggaaaac cacgaggaaa
     5101 aaatttttga aaactgggaa gaaaccgtag gcaaggatga tttggtgctg attcccggag
     5161 acatatcatg gtctttaaag cttgacggtg ctttagaaga tctcctgaag attgataaac
     5221 tgccaggcac gaaaattata ggcaagggaa accatgacta ttggtggtcc actgcctcaa
     5281 agcttgaagg actggggctt aaaaccataa agtttttgaa aaacaacttc tttaaattta
     5341 acgatgttct tgtctgcggt accaggggat gggatacaat ggaggaccat gactctgagg
     5401 ttgacaatga aaaaatattt ttaagggaac ttaacagatt taaaatttcc cttcaatcag
     5461 gaaggaagga aaagggcaaa aaaataatca tgcttcatta tccacccttc agcagagatg
     5521 gggctgccaa tgaatttttt tcgcttatga aagactatga cgcaaacatc ttaatttatg
     5581 ggcaccttca cggggaagaa ggacataaaa atgttaaaga aggtctcata gaaggtatta
     5641 tggtacattg cgtgtcaagc gattacctgg actttaaact aaaaaggata gtatgactta
     5701 agaatttccc tgccaatgac ttaacacctg ccatgttcca ggtgttttta ttatgtggac
     5761 aaaccatcta atcaccactt gacatgaaaa cataatcaga gtattatcta gttatgaaaa
     5821 tgtaccggca taagatgtat aagaataaaa aaaaggagga aacaatgttt aaagcaaaga
     5881 aatactattc tgacatattg gatgaaatca aagcaagcgg tctttggaaa aatgaaagaa
     5941 ttataacaac accccaggga agcattataa gcacaacaaa agcagacgga gtattgaaca
     6001 tgtgtgccaa caattattta gggctggctg acaataagga aataatccag gctgccaagg
     6061 atgcatacga cagatggggc ttcggcctgt cttcagtaag attcatatgc ggcacacagg
     6121 aaatccataa ggaacttgaa aaaaaactaa gcaaatttct taaaacagaa gatactattt
     6181 tgtattcatc atgctttgat gcaaacggcg gattgtttga aacaatcacc acagaaaatg
     6241 atgccatcat cagcgacgag cttaaccatg ccagcattat agacggagta aggttatcaa
     6301 aggccaaaag atacagatat aataacaatg atatgtcctc cttggaagaa cagttgattc
     6361 aggctgacaa ggatggagta aatattaagc ttatagctac agacggcgta ttctcaatgg
     6421 acgggattat tgcaaattta gaaggaatat gcgacctggc cgataaatat aatgctcttg
     6481 ttatggttga tgacagccat gcagtgggat ttgtagggaa gaccggaaga ggaacccatg
     6541 aatacagaaa tgtaatgggc agggtggaca ttattacggg aaccctggga aaagcaatgg
     6601 gtggtgccag cggcggatac accagcggca gaaaggaaat aatagactta ttgagacaac
     6661 gttcaagacc ctatttgttt tcaaatacac tggcaccggc aatagcggga gcatcactga
     6721 aggttcttga aatgctcacc gaaagcacca agtacaggga taagctggaa gaaaacacaa
     6781 aatacttcag acaggaaatt aaaaaaatcg gattgaatat tatagacagc gagcacccca
     6841 tagttccaat tatgcttgga gatgcggtcc tgtcccagaa aatggctgaa aaacttttag
     6901 aaaaaggtgt ttacgtggta ggattctact tcccggttgt accaaaggga aaagcaagaa
     6961 tcagaacaca aatttctgca gcccactcaa aagaagattt ggactttgca ctgaaagctt
     7021 tcaaagaggt taaagaagag ctgggtatat aaattaaaga aagattaagc tttgtaagat
     7081 ataattacaa agcttttatt ttaagaggga ataaataact tgaagaaata aaattttaat
     7141 tgtataataa gatttgtgta tgaaaattcg gaatttcata tatttggata aaatttttct
     7201 tgtaaaaaaa ttacagtata tgacaatctt ctacatttct cgggaaataa cccggaaatg
     7261 tgagttgttt ctataaaggg gacgcaaaat gagtgatatt gtgttgaagg aagtaaaaaa
     7321 gacaagtata ggcggtcagg cgctgattga aggtattatg atgaaggggc cttccaaaac
     7381 agctacggct gtaagaaaac ctgacggaga aatagcagta aaagaaagca gcatagcacc
     7441 aatatttaaa aataaatttt ttaaactgcc cataataaga ggaagctttg tgcttataga
     7501 agctcttgtg ggcggcgtaa aggaattaat gtattctgct gaattttttg gtgaagatta
     7561 tgaggaagat gcccttgaca aatttttgaa gaaggttttt aaggaaaagg cagacacggc
     7621 tattatttat gcttcagtag ttatagcttt ggttttttcg gttgtaatct ttatactggg
     7681 accaacattt ttgaccaacc tgttaaaagc catcatacgc aattcacttc ttctcaacct
     7741 ggtggaaggt atagtaaggg tactgctgtt tgtaggttat gtgtatatgg tttccaagct
     7801 tgaagaaata aaaagggtat ttatgtatca cggtgctgag cataaaacaa tatactgcta
     7861 tgaacacgga gaagagctta cggttgacaa tgttcgcaaa tattcaaccc tgcatccccg
     7921 atgcggaaca agttttatga ttaatgtaat gctcataagc attatagtat tttcattttt
     7981 cggctggccc aacccttttc tcaggctgct tataagactt gcaatgcttc cggttattgc
     8041 aggactctcc tatgaattga acaggtatgt gggaagatgt tcaagtgata atatttttac
     8101 taaaataatc acttatccgg gatttttgat tcaaaaaatc actacaaagg aacctgatga
     8161 tttaatgata gaggttgcta taacagctat gaataaagtt atcccccggg agggagaaga
     8221 tgacagatgg taacagtcga agaattgctg aaagccggca ctgatataat aaaaccacgg
     8281 gaattcaact cacccgggct tgattcagag ctaattttgt gttatctttt acaaaaggac
     8341 agaatctatc tgcatcttaa cagaaaaaca gaaataagcg aatatataaa agacaagttt
     8401 tatgaattgg caaaaaaacg caatgaagga tatcctcttc agtatataac caattcccag
     8461 gagtttatgg gccttgactt ttttgttcag gaaggcgtcc tgatacccag gcctgacact
     8521 gaaatattgg ttgaagcagt tatagaaaca gcaaaagcaa gatacaatga caggattaag
     8581 atacttgatt tgggaacagg cagcggagcc attgcagtaa gtcttgcata ctatttggaa
     8641 aactcatttg ttactgcaat agacataagc aaaaaggcag ttgaaacaac aaatatcaat
     8701 ataaaaaagc acaagctcaa caatataaag gttatagagg gagatatttt cgacgatata
     8761 gctgatgaaa aatttcacat tgttgtatca aatcctccct atattgaaag agatgcaatt
     8821 tcttccctcc agacagaagt ttctgtttac gagccgaaaa ttgcccttga cggaggaaca
     8881 gacggccttg actattacag aagaattgtg gaactattta agaatatcca tgaaaagaat
     8941 ggagtattag ctgtggaaat tgggtggtcg caaagaattt ccgttgaaga aatttttatg
     9001 aatgcaaaat tatttaaaga aatcaaaagc tgcaaagacc tgtccggaag agacagagtt
     9061 gtatgcggat tcttgtagct ggatccggct atattggcta tattaaaggt ttggtaataa
     9121 atcatattcg gattcagaga gaggaatgag aaataaaatg ttagaaaaat tagaagcatt
     9181 aaaagacaaa tatatagaat taggcgaaaa acttttagat cctgatacct taagtgattc
     9241 acagaagctg caaaagctta tgaaggagca ggcgcagctt gaaccggtcg tacttaaata
     9301 caatgaatat actgaagtag caaaccagct tgaagaatca aaggaaatgc ttagggaaaa
     9361 attagatgat gattttaagg aaatggtaaa ggaagaaatc aaggaattaa ctgccaggga
     9421 agaagagctg gaaaatgaca taaaaattct tcttctgcct acagatccaa atgatgacaa
     9481 ggatgtaata gttgaaataa gagcaggagt aggcggggac gaagccggat tgtttgccgg
     9541 tgatttgctc aggatgtatc tcaggtacgc agaaagaaaa ggatggaaaa cagaattcat
     9601 cacagttaac gaccagggta taggtgggta caaggaagta attttttcta ttaaaggcaa
     9661 gggagcatac tcaaagctta aatatgaaag cggtgttcac agggtacaaa gagttccgga
     9721 tacagaatcc agcggcagaa ttcacacatc ttccgcaact gtagcagtgc tgccggaagt
     9781 agatgatatt gatattgaaa tcaacccgaa tgatgtaaga gtagatgttt tccgttcttc
     9841 aggaaacgga ggacagtgtg taaatacaac cgactctgca gtcagactta ctcacatacc
     9901 aacaggtatt gtaatttcat gtcaagacga aaaatctcag ttaaaaaata aagataaagc
     9961 atttaaggtt ttaaaggcaa gactttacga caagtatctt caggagcaaa atgatgaaat
    10021 ggctcaggca cgaaaaagtc aagtggggtc gggagacaga agtgaaagaa taagaactta
    10081 caatttcccg caaggaaggg taagtgatca taggatagga cttaccttat ataagcttga
    10141 tgcatttctt gacggcgata taacagagat gcttgaagcg cttataacat ccgaccaagc
    10201 tgaaaaattg aagaacatat ctgaatgaat tttaatgtta cgtttttatg atggcaaaaa
    10261 cctggagaag tattatgaga atagacaaat ttctaaaaaa tgcacgaata attaaaagaa
    10321 gaaccattgc caaagaggca tgcgagcaag ggagagttct gattaacggc aagactgcaa
    10381 agcccggcac ggaagtggaa accggtgatg aaatagaaat aatatttggt ataaagccta
    10441 tgaaaataag agcagaaaaa ctgctagaac acacagcaaa ggatgaatac aaggaaatgt
    10501 acagcgtaat aaatgtttaa aactttagta tgcagggtat taattaatag caggatatca
    10561 gtatcttaag cagctgaagc tgctgtcata tgataaattt atatttagag gtggttaaat
    10621 ggaattaaaa ggaagcaaaa cagaacaaaa tttaatgact gcatttgcag gcgaagctca
    10681 ggctcatgtg aagtatacat actatcaaag ccaggctaat aaagatggtt atgtacaaat
    10741 cagcaagtat tttgaagaaa cagcaagaaa tgaaaaagag catgccaagc tttggtttaa
    10801 gctgctgcat gacggaatag cccacacaac agtaaactta aaggatgcag ccgacggtga
    10861 aaactacgaa tggacacaaa tgtatgcaga ttttgcaaaa gtggcaaaag aagaaggatt
    10921 caacaaaata gcttacttat ttgaaggtgt tggtgcaata gaaaagaatc acgaagaaag
    10981 atacctggca cttttgaata atattgaaac aggaaaagta ttcaaaagag aaggggaaat
    11041 tgtgtggcag tgtaacaact gcggccatat ccacgttggg gcagaggctc cgaaggtatg
    11101 tcccgtatgc gaccacccac aggcacattt ccaaatttac acaaaaccat attaatattc
    11161 ataatacaag agaaaatgct ccccaatccg gggggcattt gtttttatga ttataaatta
    11221 aaaaattgtc taaagaaacg acaaattttt acgatttttt aatattaaat atgtttaaat
    11281 attagtcgca atgtggtatt gtaattttaa attttaatcg cggggaatca acataaaatt
    11341 tagaacataa gcataaataa taatttatat acttatgcat aaatattaaa taatgaaatt
    11401 tagttttaaa taagtaaaag gagtttttat ggatagcaat acacatgaag cagttaaact
    11461 tacacaggaa ctgatacgga ttcccagtca taaatatgtt gaaaacaggg aatcaaatat
    11521 agctaaattc atttatgact attgcagcaa aaagggattg gaagttgaat ttcaagaagt
    11581 ggaagggctt aggagaaatg taattgcaag gctaaaggga aacggaaccg gcaagaatct
    11641 aattttcaac ggacatacag atactgtacc cccatacgag atgacaattg aacctttcgg
    11701 cggtgagata aaagatggtt atgtattggg aagaggtgcc aatgatatga aaggagccgt
    11761 ggcttgcatg attacagcca tggtaaatat taaaaacaaa ggcatagtat taggcggaga
    11821 tattatttta actgctgcag tgggggaaga agaaaaaagc gacggtacag aatatgtagt
    11881 aaaaagcggg atcacggcag atggggctct tgtgggagaa cctgcaaact atggatatgc
    11941 tcttgggcac aggggcttgg agtggcttga aataaggatt gaaggcagga cagcccacgg
    12001 aggaatacct gaattgggga ttaatgctat ttcaaaggct gccaggctta tcaggaaaat
    12061 tgaagaagat ctgatgccta agcttaaaga aaggcagaat gaatggatgg gtccatcggt
    12121 aatgaacttc ggcctcataa agggaggcac tcagccaagc tctgttgcgg acagctgtat
    12181 tattcaaata gacaggcgat atttacctga ggaaaacgtt gaaagtgtaa taaaagaata
    12241 tcaggatatc attgatgaat taaaacagga ggaccctgac ttcaaagctg aaataataag
    12301 gatggattca aatttgatgg aggaatttga ccatgctcct ttaattgcac agccggattc
    12361 tgatatagca aggacagttt acaatgtatt gaaggatttt aataaaaagg agcctaatat
    12421 tgaaaaaaga agaggatgga cagatgccgg agttttatca acctatggca aaatacctac
    12481 tgttgtaaca ggaccgggag acttaaagta ttcacatgcc agggatgaga aaattcctgt
    12541 gcaggacctg gtaaattatg ttgaaattta cacaaggatt gctgagaaat tttgcaagta
    12601 agaaggagga ttagatgctt gatattaaaa tcattaacgg aaaaataatc gatgtagaga
    12661 ataaaaggat agttgaagga gatataggca tcaaggacgg aaaaattaca gatataggct
    12721 cagttcctga tgagggaaag gttgaaatag acgcagacga aaaatatgta tctcctggat
    12781 ttattgatat tcatatgcat gaggaggatt ttaccctcac taaaaaggaa gaatatgata
    12841 tatccgatac catgctcaat atgggagtaa ctacctgtgc agtaggtaat tgcggcaaca
    12901 acaggcagag cattaaagag ctttatgaat ttataaatga aaaaggcaat cctgtcaatt
    12961 atcttaccta tatcggtcat aattttttga gagttgaagc aggcaacacg gacatataca
    13021 aaagatcctc aagggaacaa attgaaaaaa tgcagaaatg ggttgcagat gcagttgatt
    13081 ttggcgccat aggagtatct tacggcttgg aatactgccc gggaatcgac actgaggaag
    13141 caattgcaat aacaaaggaa attcaaggaa gagatgattt gctacttgct gcccactaca
    13201 gaaaggatgc aatccatgcc cttgattcca taaacgaaat ggcttatata ggaaaggaaa
    13261 ctaaaattcc gttccagatt tcacatctgt caagctgcag cgcctttgga aatatgactg
    13321 aagctttgaa tcttattcag gaaattcacg acagtggaac aaatattatg gttgattcct
    13381 atccatatga tgcattcagc acctacatcg gctctgccgt atttgacgaa ggatgctttg
    13441 aaacatgggg caaaagctat gaagctataa tgctgaccga agaaccttac aagggtattt
    13501 attgtgacag ggaattattt gaaaaagtac gtaaggaata tcctaaaatg ctggttgttg
    13561 ccaatgttat gaatgaggaa gaaataattg aagcattgaa ccatcacctg gttatggcag
    13621 ccagtgacgg catttacaga aaccattcag gccatccaag gggggcagga acattcccaa
    13681 ggattatagg gcgctttgta agagatatga aggtgatgga attctttgat gctgtttaca
    13741 aaatgaccta catgccggca aaaagattaa aacttaataa aaaaggtatc ttaaaagcag
    13801 gctatgatgc tgatataact atatttaact atgatacaat aatagacaaa gcaacatttc
    13861 aggagcctca gctcaggcct gaaggtatag actatgtaat tgtggacggt aaggttgcta
    13921 ttgaaaaggg aataaccgtc aacaatacac tgggtaaatt ctataagagg ggaggagctt
    13981 aataaagaaa ccgctcgcac tgagcatata gaaatgttac tataacaaag tactaattaa
    14041 tttttaaaaa aacaggagga tttatggaag caaaaaagaa aaaattcaaa gtaccgcata
    14101 cctatgtaat tttgttcgca atgattatta ttatggcact attaacttat gtaattccag
    14161 caggccaata tcaaaaagtt gaaggccccg gcggaagaat gatcgttgac ccaagctctt
    14221 atgcaagtgt ggaagcaagc ccggcaatgc cttgatattt taaaagcatt tcctaaagga
    14281 ctcgccgcag cacaaagtat cgtatttttc atattcatag ttggaggctc ctttaacata
    14341 gttaaccaaa caggagctat tgaggcaggt ataagcaaag ttgctttaag cctcaaaggt
    14401 aaagaaaaac tgatgattcc tataatagta tttgtattct ctctaggagg ctctactata
    14461 ggaatggcag aggaggcaat tgtattcgtt ccaattggtg ttgctttggc aagagctctt
    14521 ggttacgacg ctgttacggg tatggcaatg gtttcgcttg gagcagcggc aggatttact
    14581 tcaggtttca tgaatccttt tacagtagga gttgctcagg gaattgctga attaccttta
    14641 ttctcaggta tcggtttaag aattattatt tggatttgta cattaatttt ggtaatcggt
    14701 tatatctaca gatatgctaa caaggttaaa gcagatgtta aaaacagtta cgtttatgaa
    14761 cttgaaatgc aggaaaaaga tatggctata gacttaagca acgtaagaaa aatgactaaa
    14821 agagatactg cggttctttt aatattcgtt gttggtatgg ctatactaat attcggagtg
    14881 tttaaatacg gatggtatat tacagaaata gctgcaatat tcctggcaat aggtatattc
    14941 tcaggtattg cagcagctat gcctatagat gatattgcaa aacatttcat tcttggtgcc
    15001 aaagatatga caatgggagc attggttgtt ggtttggcaa gaggaat
//
LOCUS       DAOPUT010000040        14739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_4156_length_14739_cov_18.809861, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000040 DAOPUT010000000
VERSION     DAOPUT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14739
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4156_length_14739_cov_18.809861"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..335)
                     /gene="rpoZ"
                     /locus_tag="PKK75_05005"
     CDS             complement(12..335)
                     /gene="rpoZ"
                     /locus_tag="PKK75_05005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNZ82989.1"
                     /translation="MKKISIDELNEMVDSQYSLVTIISKRARQIIDGSEPLVKTSTKK
                     PMAIAIEEFYNEKFEPIYDYDKFIKSPGENKETQDETVETAVKETAVMEETTAQEKSD
                     NQSND"
     gene            complement(338..943)
                     /gene="gmk"
                     /locus_tag="PKK75_05010"
     CDS             complement(338..943)
                     /gene="gmk"
                     /locus_tag="PKK75_05010"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNZ82990.1"
                     /translation="MDEGLLVVISGPSGAGKGTICKRLLMEMSYLKVSVSATTRKPRE
                     GEAEGISYYFIDEEEFIKRINNDEFLEYAKVYGNYYGTPKEEVFKQLKAGNDIILEID
                     IQGALQVKKNYPMGVFIFILPPSLAELKTRIEGRGTDSKEVILRRMESAYDELNYAFQ
                     YDYAVLNDQVEVAAEKIKHIIEAEKNRVIRKKEIISKIREI"
     gene            complement(953..1201)
                     /locus_tag="PKK75_05015"
     CDS             complement(953..1201)
                     /locus_tag="PKK75_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNZ82991.1"
                     /translation="MSNTINIGFGNIALVNRILAVVSPESAPIKRFIQESREKGKLID
                     ATYGRKTRAVIITDCDYIILSAIQPDTMAQRFEDHNTK"
     gene            complement(1211..2095)
                     /locus_tag="PKK75_05020"
     CDS             complement(1211..2095)
                     /locus_tag="PKK75_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNZ82992.1"
                     /translation="MANIFSMTGYGKGESNDGKRSITAEIKTINNRYCDINIKTPRHL
                     RFFEDNIRKILKNSLQRGRIDVYINIDYITESDTVILPNLALARQYKDAIDKIKQELG
                     MNSSPSLDTIIKFQDVLIAKEDDPDDDELRVCLEGAMNEAVKNLLAMRAKEGMELKSD
                     ISLSAGRILELMEVISENSKTIVEEYKEKFENRIKELLGSHEFDENRLYNEIVIYADK
                     SDINEEIVRFNSHMVQLSDALDLGGTIGRKLDFIIQEANREINTIGSKVGNLDIIQVV
                     IEIKNLLEKIREQIQNIE"
     gene            2369..>3555
                     /locus_tag="PKK75_05025"
     CDS             2369..>3555
                     /locus_tag="PKK75_05025"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNZ82993.1"
                     /translation="MSLDGIFLNSIINELNNKLSGGRVDKIHQPDKNEIVLSIRSKGE
                     NYKLLITAIGSSPRLHLTNITRNNPAEPPMFCMLLRKHLTGAHIRGVRQINFDRIVEI
                     SFECRDELGTVVHKSLITEIMGKHSNIIFINESNNTIIDSLKRIAENVSSVRQVYPGL
                     KYAAPPGSDKLNPMTVTGDLFMKEIQSVNSGIYIFDFLYKNFTGFSPFISREICYLAN
                     LNESTCLGELNGRNKEEIWTAFCKIIDKINQGDFSYNIYYDENKPCGFYCMEIEHLKK
                     YDKKSFSSPGELLDEYYHDLDNKNKINQRVSNFIKSINTKISRNKKKAEKQRNELLNA
                     EDRDKYMVYGQLILSNLHKTPENNKLAVVNYFDPEEKEILIPLDPKFSLSQNSQKFFK
                     KYN"
     assembly_gap    3556..3655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3656..3850
                     /locus_tag="PKK75_05030"
     CDS             <3656..3850
                     /locus_tag="PKK75_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ82994.1"
                     /translation="EAARIAAFYSKGKTSDTVEVDYTKKSNVKKPPNAKPGFVIYDTN
                     YSMLAEPNIEGIKRGSKDMT"
     gene            complement(3895..4425)
                     /gene="pyrR"
                     /locus_tag="PKK75_05035"
     CDS             complement(3895..4425)
                     /gene="pyrR"
                     /locus_tag="PKK75_05035"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNZ82995.1"
                     /translation="MNIKALIMDEKAIERAVFRIAHEIIERNKGIENIVLIGIKTRGW
                     PLSLRLANKIEEIEGSKVPVFPLDITYYRDDLEEDDRAPLAVDSFDFNIKNKTVVLVD
                     DVLYTGRTVRAALDAIVDRGRPQNVELAVLIDRGHRELPIRADFIGKNVPTSRSERIS
                     VQLEETDKINQVVITE"
     gene            complement(4551..5609)
                     /locus_tag="PKK75_05040"
     CDS             complement(4551..5609)
                     /locus_tag="PKK75_05040"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ82996.1"
                     /translation="MKEYKSKDLIHDEADKNTENSGHIENTENFDDSDDFDDSDDFED
                     SYCYTDDFKKEIILVSERENQRIDLFVSEKLGSSRNSVQKLISDGNVTVNENAVKANY
                     KIKTKDTIKITIPPPAVLDVQAENIALDIIYEDRDLAVINKAQGMVVHPAPGHYSGTM
                     VNGLMYHLKDLSTINGVLRPGIVHRLDKNTSGLMLVAKNDKAHCFLAQCLKEHSINRI
                     YYALVDGNVKEDQGEINAPLGRSDKDRKKYVVTNKNSKEAITDYWVVERYEKYTLLKL
                     KLKTGRTHQIRVHMKHIGHPVVGDDVYGKTDKFGLEGQLLHSKILGFVHPSTNKYMEF
                     ESELPDNFLRVLNKIKVL"
     gene            complement(5596..6078)
                     /gene="lspA"
                     /locus_tag="PKK75_05045"
     CDS             complement(5596..6078)
                     /gene="lspA"
                     /locus_tag="PKK75_05045"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNZ82997.1"
                     /translation="MNIIAAIIIVFSVVSDRLTKFWAADVLKNGGSIKIIGNFLRFTY
                     AENRGAAFSILQDQRMFFLITTVIMLIVLWVIYFRTKNLSKLSKLSIAMITGGAIGNF
                     IDRYRFGFVIDFIDVRFGNIYNFPVFNIADSFVVCGTLLMVILILFNRFETSGSDERI
                     "
     gene            complement(6089..6871)
                     /locus_tag="PKK75_05050"
     CDS             complement(6089..6871)
                     /locus_tag="PKK75_05050"
                     /inference="COORDINATES: protein motif:HMM:NF037631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HNZ82998.1"
                     /translation="MKDLEKYFDYIKDDETRNTVKKIQDKASYVNKNHITAITEFINP
                     YVEKLSIPVIKSYNIEFEIFPSYENCERKVFILYPQYSEIDASEYVSGIHIVNKSKFK
                     KLNHKDYLGALMSLGIERSRIGDIFVYDEYGDVIVQSDISDYIIYNLDKIGHNRIEAE
                     RINIKQVGFKEQEHEIINIISSSMRVDNIVKHLTNSSREKASITIKSGSVKINHLPED
                     KTSTELKEGDLLSIKRAGRFKIYKVYGTTKSGKQKIQIKHYV"
     gene            complement(6900..7163)
                     /locus_tag="PKK75_05055"
     CDS             complement(6900..7163)
                     /locus_tag="PKK75_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNZ82999.1"
                     /translation="MFYTIKIALGLLFRIIDSLILIRVILSFFPTLQSSRISYFIYQM
                     TEPVLAPCRAILDRLGLGMGMIDFSPLVALILLNILQRLIYTL"
     gene            complement(7173..7610)
                     /locus_tag="PKK75_05060"
     CDS             complement(7173..7610)
                     /locus_tag="PKK75_05060"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNZ83000.1"
                     /translation="MSMMEKVKDFIGITDLEEDYEEEEVVSEGTERNKNERMETYTKR
                     NNVIKVHSNTDMKVFICEPEKYEDCTKAVDEIKNRKVVVLNIEGMELENQKQVFEFIK
                     GAVYALEASIQKVSNGIFVLAPNNVQIDGRLSDRYERNFYYGK"
     gene            complement(7611..8315)
                     /locus_tag="PKK75_05065"
     CDS             complement(7611..8315)
                     /locus_tag="PKK75_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNZ83001.1"
                     /translation="MSIKENIDSIMKKIDTACKKTGRNPKDITLLAVSKTVESDRAKE
                     AVEAGIKNLGENKVQELVKKYEELKNINVKWHMIGHLQKNKVKYIIDKNILIHSVESL
                     SLAEEINKRAKNSNLTADVLIELNIGEEESKFGINEENVYDFIRSMEQFENIRVLGLM
                     TVAPFCENPEDVRWVFKTMKNIYSKISIMNLRNTEMKYLSMGMTNDFEIAIEEGSNII
                     RIGTAIFGARKYKEDE"
     gene            complement(8330..9556)
                     /locus_tag="PKK75_05070"
     CDS             complement(8330..9556)
                     /locus_tag="PKK75_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNZ83002.1"
                     /translation="MARKNFGLKNFFLSFVIVIIAIYIFQNFKSPADFMVAENGILED
                     VIKAEGIVVKDEKVYNAAIDGSVIYYHEEGSKIKEGQLLLDINTDADSAVVNRQIAEI
                     QSTIDKLENSSQVLSQENFTAYQNDLQISILNNELQDVYNIAVQMTSNEFYNSKYEGY
                     TVSQLKEMKNSLTNSVSTHKVPYYSDRAGIISYKTDGLEDFYIFETVMDMTPVTTIRH
                     EYTISDKSDTVKKDESFYKIIGSFEYYIVVTVNNEKAKLFEENKYIKVRILSEGGQHI
                     VWGYIKKINYGSEESVLMLYFDDYFHKVYDKRYVNIELITATYEGLKIKNRALIEKDG
                     MKGVFVQDASNIIKFFPVVLLGSDEEYSVVYPGEYIGVNERNIITIREQNYPTIKIFD
                     KVILQPDKVYEGQIAD"
     assembly_gap    9851..9950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9951..11371)
                     /gene="spoIVA"
                     /locus_tag="PKK75_05075"
     CDS             complement(<9951..11371)
                     /gene="spoIVA"
                     /locus_tag="PKK75_05075"
                     /inference="COORDINATES: protein motif:HMM:NF021089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HNZ83003.1"
                     /translation="MGVVGPVRTGKSTFIRKFSESVMLPNIADEYTKERAKDEMPQSG
                     TGKTVMTTEPKFVPGEAVAVTFNNNIKAKLRLIDCVGYMIPGAIGNMEGEIPRLVSTP
                     WNEEPIPFAEAAEIGTKKVINDHSTIGVLVTTDGSITDINRDNYIMAEERVVRELKEI
                     NKPFVIILNTTHPNNENTVALSAQLKEKYDAPVLIVDCMNLKTDDIEDVLENVLYEFP
                     IKEINVDLPKWFDGLPYDYSMKKDLINILRDDFQNYYKLSDFNAEDIFSEENDYVDDY
                     YVKDIELGTGKINLAVDLDNSYYYKIISELSGEEITGEHQILKLIGDLSKTRQSYSRV
                     EAAINEAKASGYGYVTPTVADMIIESPEVFKEGGRYGIKIKAQAPSLHIFSAGVNAEV
                     SPVIGSKAQSEDLISYLIGQMKENPQSIWQAEMFGKPLSSLVEEQLDSKLTAMPDNAR
                     KKLKKTIEKIVNDGQGSIIFLIV"
     gene            complement(11593..>12096)
                     /locus_tag="PKK75_05080"
     CDS             complement(11593..>12096)
                     /locus_tag="PKK75_05080"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNZ83004.1"
                     /translation="IFMSEYFRVYTNEDVKGAELGGALKNIIALGAGISDGVGYGDNA
                     KAALMNRGIVEIARLGIAMGADIHTFYGLTGIGDLIVTCTSRHSRNWNAGYLIGQGLS
                     KDEAIKKVGMVVEGIPTTYAAYWLSKKLNVDMPIVDAMYDILENKADVKETVNKLMLR
                     DKKEEKL"
     assembly_gap    12097..12196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12427..13752)
                     /gene="der"
                     /locus_tag="PKK75_05085"
     CDS             complement(12427..13752)
                     /gene="der"
                     /locus_tag="PKK75_05085"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNZ83005.1"
                     /translation="MGRPVVTIVGRPNVGKSTLFNRIAGRRISITEDTPGVTRDRIYA
                     ECEWLDNHFTLIDTGGIEPNTTDTIFEQMRIQAEIAIDMADVVLFMVDGKEGLTADDT
                     EVAQMLRKADKEIILVVNKMDRFVNEDNIYEFYNLGLGDPVSISSAEALGIGDLLDEI
                     ISKFDERMNVEEGDDSVKIAVIGKPNAGKSSIINKIIGEDRVIVSDIPGTTRDAIDTK
                     FERNSKKYTLIDTAGIRKKNKILESVEKYSVLRAFTAVERADICILVIDADKGVTEQD
                     IRIAGYSHDNSKGMIIAVNKWDLIEKDNKTFKEFTDDIRQKLAYASYAPIITVSALTG
                     QRMNKILDAVDEVYSFRNLRISTGVLNDILTEAVLMNQPATVKGKRLKIFYGTQVAVN
                     PPKFLIFVNDKEIVHFSYERYIENKIREAFEYKGTPIIIEFRNRSEKNL"
     gene            complement(13765..>14739)
                     /locus_tag="PKK75_05090"
     CDS             complement(13765..>14739)
                     /locus_tag="PKK75_05090"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HNZ83006.1"
                     /translation="YLKDDDTRLSFLYGNFITLTNLSDDEIDRIIKYRISPIKVSVHT
                     TDLELRKYMMGNDKDMDILKYLKRLVDAHITVDCQIVLVRDVNDKDRLTKTITDLSSL
                     HPGLRSVAVVPVGLTRHREKLADLKPFDKESAQEVVRQVENIQERLLRKLGTRFVFIA
                     DEFYILSGNGFPSYEEYEDFDQLENGIGMCRLFISSIDEDIKNLCYEKVLKEEATFVT
                     GAAAYDLMVDTANKIMSRINVKINVVKIINNYFGDKITVSGLITGKDIIDQLKGKNYK
                     NLILPGNILNDNNVMLDDVAIEDLERELNARIEICDVETDSLLGLIVK"
BASE COUNT         4024 a         3006 c         2173 g         5236 t
ORIGIN      
        1 aaattaaata atcagtcatt tgactgatta tctgactttt cttgtgccgt tgtttcttcc
       61 ataactgctg tttctttaac tgctgtctca accgtctcat cctgtgtttc tttattttcc
      121 cctggtgatt ttatgaattt atcataatca taaattggct caaatttttc attatagaat
      181 tcttcaattg caattgccat tggtttcttt gtgcttgttt tgactaaagg ttcagaacca
      241 tcaattattt gcctggctct tttagatatt attgtcacta aagaatattg gctgtcaacc
      301 atttcattta attcgtctat tgatattttt ttcattctca tatctccctg attttactga
      361 ttatttcttt tttccttatt actctgtttt tttcagcctc tattatatgt ttaatttttt
      421 cagctgcaac ttctacctga tcattaagga ctgcatagtc atattggaaa gcgtagttta
      481 gttcatcata ggctgattcc attctacgta atataacttc cttcgaatca gtgcctcttc
      541 cttcaatcct tgtttttaac tcagccagcg atggaggaag aataaatatg aacactccca
      601 ttggatagtt cttttttacc tgcagagctc cctggatatc aatttccaga attatatcat
      661 tccctgcttt aagctgttta aacacttctt cttttggtgt tccgtaataa ttgccgtata
      721 cttttgcata ttcaagaaat tcatcattgt ttattctttt gatgaattcc tcttcatcaa
      781 taaaataata gcttatgcct tctgcttcac cttctctggg cttcctggtt gttgcagata
      841 cggaaacttt caggtaactc atttccatca gaagtctttt acatatagtt cccttgcctg
      901 ctcctgaagg gcctgatatt accactaata atccttcgtc cattgtgcac ctctacttag
      961 tattgtggtc ctcgaacctc tgtgccattg tatccggctg aattgccgat agaattatat
     1021 aatcacagtc agttataatc acagctctgg tttttctgcc gtaagttgcg tctataagct
     1081 tccccttttc ccggctttcc tgaatgaagc gttttatggg tgcagattca ggacttacaa
     1141 ccgccaatat tctgttaact agtgctatat taccaaaccc tatgttaata gtatttgaca
     1201 tatttgactc ctattcgata ttttgtattt gttcccttat tttttccaaa agattcttaa
     1261 tctcgattac gacctgtata atatccagat tgcctacctt tgagcctatg gtatttattt
     1321 ccctgtttgc ttcctgtatg ataaagtcaa gcttcctgcc tattgtgccc cccaggtcca
     1381 aggcatctga caattgtacc atatgagagt taaatcttac gatttcttcg ttaatatcac
     1441 ttttatcagc ataaattaca atttcattgt aaagcctgtt ttcatcaaac tcgtggcttc
     1501 ctaagagctc cttgattcta ttctcaaact tttccttgta ttcctctaca atagttttgg
     1561 agttttcaga tattacttcc attaattcaa gaattctacc tgcgcttaag ctgatgtcag
     1621 acttaagttc cataccttcc ttagcccgca ttgccagaag attttttaca gcttcattca
     1681 ttgcaccttc cagacacact cgaagttcat catcatcagg atcatcttcc tttgcaatga
     1741 gaacatcctg aaattttatt attgtatcaa gagaaggact ggagttcatt cctaattcct
     1801 gtttaatttt atcaattgcg tccttgtact gccttgctaa agccaaattc ggaaggatta
     1861 ccgtatctga ttctgttatg tagtctatgt tgatgtatac atctattcta cccctttgca
     1921 ggctgttttt caagattttt ctaatgttat cctcaaaaaa ccgcaaatgt ctgggagttt
     1981 ttatgtttat atcgcaatat ctgttgttga tagtttttat ttctgcagta atgcttcttt
     2041 ttccatcatt gctttcacct ttgccgtaac cggtcatact gaatatattt gccatttttc
     2101 tctccgatat tataattgtt atctttttac atatgctgac attttactac gggacatatt
     2161 cccgcatctt attacaccat tataacatca aaagcttaat agtaaacaaa aaaatggcag
     2221 tttatgtccc ttgcattcaa atattttaaa aaaatcatga cattgcagca tttataatat
     2281 acaatatggt tattactaat ataattcagt attaaaaacg attaggaaaa tctttatagt
     2341 ttaaattttt ggaggtaaca aggataaaat gtcactggac ggtatttttc taaacagtat
     2401 aataaatgag ctcaataaca aattatcagg cggccgggtg gataaaattc atcagccgga
     2461 taaaaatgaa atagtattat ctataagaag caagggtgaa aactacaagc tgttaattac
     2521 tgccataggc tcaagcccca ggctccatct tacaaatata acaagaaata accctgcaga
     2581 acctcctatg ttttgcatgc tcttaagaaa gcacctgaca ggtgcccata taagaggtgt
     2641 aaggcagata aattttgaca gaattgtaga aatatcattt gaatgcaggg atgaattagg
     2701 aaccgtggta cataaatccc tcattacgga aataatgggt aaacacagca acattatctt
     2761 tataaacgaa agcaataata cgattattga ttctttaaaa aggattgccg aaaatgtaag
     2821 ctctgtaaga caagtttacc cggggcttaa atatgcagct ccccccggct ctgataaatt
     2881 aaaccccatg actgtaaccg gtgatttgtt tatgaaagaa attcagtcgg taaacagcgg
     2941 aatatatatt tttgattttt tatataagaa ttttaccggc ttcagccctt ttatatcaag
     3001 agaaatatgt tatctggcaa acttaaatga aagtacctgt ttaggtgaac taaacggtag
     3061 aaataaagaa gaaatatgga ctgcattttg taaaatcata gacaaaataa atcaaggaga
     3121 cttttcctat aatatttatt acgatgaaaa caagccatgc ggtttctatt gtatggaaat
     3181 tgaacattta aagaaatatg acaaaaaaag cttctcttca cccggtgaac ttcttgatga
     3241 atattatcat gatttagaca ataagaacaa gataaaccag agggtatcaa attttattaa
     3301 aagtataaat acaaaaattt caaggaacaa aaaaaaggct gaaaagcaga gaaatgaact
     3361 gctaaatgcc gaagacaggg ataaatacat ggtttacggc cagcttattc tgtcaaacct
     3421 ccataaaact cctgaaaaca acaagctcgc ggttgttaat tactttgacc ctgaggaaaa
     3481 ggaaattttg attcccctgg accctaaatt cagtttgtca caaaacagcc aaaagttttt
     3541 caaaaaatac aacaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaagc
     3661 agcacgtatt gctgcatttt acagcaaagg gaaaacttca gacactgttg aagttgacta
     3721 tacaaaaaaa tcaaatgtta aaaagccccc aaacgcaaaa ccgggttttg taatatacga
     3781 cacaaattat tcaatgcttg cggagccgaa cattgaaggt ataaaaaggg gcagcaaaga
     3841 catgacttga ttgttctgat gagagatagc agggataatt cccctgtctt cagcttattc
     3901 tgttataacc acctgattta ttttatctgt ttcttcaagc tgtacgctta ttctttcact
     3961 tcttgatgtg ggcacgtttt taccaatgaa atctgcccgt atgggaagtt cccggtgtcc
     4021 tctgtcaatt aatactgcca gttctacatt ttgaggcctc ccccggtcca cgatggcatc
     4081 aagtgctgcc cttaccgtcc tgcctgtata taatacatca tccaccaata ctacagtttt
     4141 atttttaatg ttaaaatcaa agctatcaac cgccagtgga gctctatcat cctcttccag
     4201 atcatctctg taataggtaa tgtccaaagg aaaaacaggt acttttgaac cttcgatttc
     4261 ctcgatttta tttgccagtc tgagagacaa gggccatccc cttgttttta taccaatgag
     4321 cactatattt tcaataccct tatttctttc tataatttca tgtgcaatcc tgaatactgc
     4381 tctttcaatt gctttttcat ccattattaa tgctttgata ttcatagtta cctcccgttt
     4441 tatttcctta tatcatactg ggtgttaaaa agtatttctt cagaagcagg gggatgcacc
     4501 ttcaaagtca ggtgactctt tgtaagatgt tttgaaggaa tgccccaagt ctataagact
     4561 tttattttat tcaatactct taaaaaatta tccggtaatt cactttcaaa ttccatatat
     4621 ttgttggttg aagggtgaac aaatcccaaa atcttcgaat gcagaagctg cccttccaaa
     4681 ccaaatttat ctgttttccc gtaaacatca tcccccacca caggatgacc gatatgtttc
     4741 atgtgcaccc ttatttgatg ggttctgccg gttttcagct ttagtttaag caaggtgtat
     4801 ttctcatacc tttcaaccac ccaataatct gtgattgctt ccttgctgtt cttatttgtt
     4861 acaacgtatt tttttctgtc tttgtcgctt ctccccaaag gtgcatttat ctcaccttga
     4921 tcttctttta catttccatc aaccaatgcg taataaattc tgttaattga atgctccttt
     4981 aaacactgag ccaggaaaca gtgagctttg tcattttttg caacaagcat caggcctgaa
     5041 gtatttttgt ccagtctgtg gacaatcccg ggccgtaaaa ctccgttaat ggttgataaa
     5101 tctttaagat ggtacataag tccgtttacc atagtgccgg aataatgtcc gggggcagga
     5161 tgcaccacca ttccctgagc tttgtttata accgccaaat ccctgtcctc ataaataata
     5221 tccaaggcta tgttttctgc ctgcacatcc aaaactgccg gaggcgggat tgtaatttta
     5281 attgtatcct tagtttttat cttgtaattt gcctttacag cattttcatt aactgtcaca
     5341 ttcccgtctg aaataagctt ctgtactgaa tttctagaac tccccagttt ttccgaaaca
     5401 aataaatcaa ttctttggtt ttctctttct gaaactagga tgatttcctt tttaaaatcg
     5461 tcagtataac aataagagtc ttcaaaatca tcagaatcat cgaagtcatc tgaatcgtca
     5521 aagttttctg tattttctat atggccggaa ttttcagtgt ttttatccgc ttcgtcatga
     5581 attaaatcct ttgacttata ttctttcatc gcttccactc gtttcaaacc tgttgaataa
     5641 aatcaaaata accataagca gggttccgca taccacaaaa ctgtctgcta tgttgaaaac
     5701 aggaaaatta taaatgtttc caaatcttac atcgataaaa tctataacaa atccaaaccg
     5761 gtacctgtca ataaagttgc ctattgctcc gccggtaatc attgcaatgg acaacttgga
     5821 caatttggaa agattctttg ttctaaagta tataacccat aaaacaataa gcattattac
     5881 agtagtaatc aaaaaaaaca ttctttggtc ctgaagtatg ctgaatgccg cacctctgtt
     5941 ctctgcatag gtgaaacgca agaaatttcc tataattttt attgagccgc cgttttttaa
     6001 aacatctgct gcccaaaact ttgttaaccg gtctgaaaca accgaaaata caataataat
     6061 cgccgctata atattcataa atctcctgtt aaacatagtg cttgatttgg attttttgtt
     6121 tgccgctttt tgtggtaccg tatactttat atattttgaa ccgtcccgct cttttgattg
     6181 acaataagtc cccttctttt aattccgtgc ttgttttgtc ttcaggaagg tggttgattt
     6241 taacactgcc tgattttatt gttattgatg ctttttctct tgatgaattg gtaaggtgct
     6301 ttacaatatt gtcaaccctc attgaggaag aaataatatt gattatttca tgctcctgtt
     6361 ctttaaaacc cacttgtttt atgtttattc tttctgcttc tattctatta tgacctattt
     6421 tgtccaaatt gtaaattata taatcggaaa tgtcagattg tacaattaca tctccatact
     6481 catcatatac aaaaatatca ccaatcctgc ttctttctat acctaaggac atgagggccc
     6541 ccaggtaatc cttatggttt aacttcttga atttagactt atttacaatg tggattcctg
     6601 aaacatattc acttgcatct atttcagaat attgcgggta taaaatgaaa actttcctct
     6661 cacaattttc atatgagggg aaaatttcaa attctatatt ataacttttt attaccggaa
     6721 tacttaactt ttctacatag gggtttatga attctgttat tgcggtaata tggtttttat
     6781 ttacatatga tgccttgtct tgaatctttt taactgtgtt tcttgtttca tcatccttga
     6841 tgtaatcaaa atatttttct aaatctttca tttttcacct tctagcagct tcacgcatct
     6901 catagtgtat atatcaatct ctgcaatata ttcaataata taagagccac caggggagaa
     6961 aagtcaatca ttcccatgcc taatcccagt ctatctaaaa ttgccctgca aggagccagt
     7021 acaggctcgg tcatttgata gataaagtag gaaattcttg aagattgtag ggttgggaaa
     7081 aaggataata tcactctgat cagtattaaa gaatcaatga ttctaaatag taatccaaga
     7141 gcaattttta ttgtataaaa cataaacgct ccttatttgc cgtagtagaa atttctttca
     7201 tatctgtcgc ttaatcttcc gtcgatttgg acattgttgg gagctaatac aaatattccg
     7261 tttgacactt tttgtatact tgcttccaaa gcataaacag cgcctttgat gaactcaaac
     7321 acttgcttct ggttttcaag ttccatccct tcaatattaa gaactacaac ttttctgttt
     7381 ttaatttcgt caacagcttt tgtgcaatcc tcatattttt ccggctcaca tataaagact
     7441 ttcatgtcgg tgttggaatg aaccttgatc acattgtttc tttttgtgta ggtttccatt
     7501 ctttcatttt tgtttctttc tgttccttca gaaacaactt cctcctcctc atagtcttct
     7561 tccaggtctg tgatgcctat gaagtctttt accttttcca tcatgctcat ttactcatcc
     7621 tccttatatt ttctggctcc aaatatggct gttccgatac gaataatatt tgagccttcc
     7681 tcgatagcaa tttcaaagtc atttgtcatg cccatggata aatatttcat ttctgtgttt
     7741 cttaaattca tgattgaaat ttttgaatat atattcttca tcgttttgaa aacccacctt
     7801 acatcctcag ggttctcgca aaacggggca accgtcatca atccaagtac tcttatattt
     7861 tcaaactgct ccatgctcct tataaaatca taaacatttt cttcatttat gccaaatttg
     7921 ctttcttctt ctccaatgtt aagctctatc agtacatctg cagtcaaatt actattcttg
     7981 gctcttttgt ttatttcctc tgccaggctt aaactttcca cagaatgaat caggatattt
     8041 ttatctatta tgtattttac cttatttttc tgtaaatgac ctatcatgtg ccatttaaca
     8101 tttatgttct taagttcttc gtacttttta acaagctcct gtactttgtt ttctccaaga
     8161 ttcttaattc ctgcttcaac agcttccttt gccctgtcag actctactgt ttttgaaaca
     8221 gcaagcaatg taatatcttt cggattcctt ccggtttttt tgcaggcagt gtctattttt
     8281 ttcattatac tgtcaatgtt ttctttgatg ctcaactttc atttgcctcc taatccgcta
     8341 tttgaccttc atacaccttg tcaggctgga gtattacttt atcaaatatt ttaattgtag
     8401 gataattttg ttctctgatt gttattatgt ttctttcatt gacaccaata tattcaccgg
     8461 gatataccac ggagtattct tcatcgcttc ctaataatac tacaggaaag aattttatta
     8521 tattgcttgc atcctgaacg aaaacacctt tcatcccgtc tttttcaata agtgcccggt
     8581 tcttaatttt aagcccttca tatgttgcgg tgataagctc tatgtttacg tatcttttgt
     8641 catatacctt gtggaaatag tcgtcaaaat aaagcattaa tactgattct tcgctgccat
     8701 aattaatttt tttaatataa ccccagacta tgtgctgtcc gccttccgag agaattctga
     8761 cctttatgta tttattctct tcaaacaatt ttgctttttc gttatttacc gtaacaacaa
     8821 tataatactc aaaactgcct attatcttgt aaaagctttc atctttttta acagtatcgc
     8881 tcttatcgct tattgtatac tcatgtctta tagtggtgac tggtgtcatg tccatgacag
     8941 tttcaaatat ataaaaatct tccagcccgt cggttttata tgatataatt ccagctctgt
     9001 cagaataata tggaacctta tgagtagaaa cactgtttgt cagactgttt ttcatttctt
     9061 taagctggct tacagtatac ccttcgtatt tactgttgta aaactcattg ctggtcattt
     9121 gtaccgctat attgtagaca tcctgtagtt cattattcaa tatgctgatt tgcaaatcat
     9181 tttgataagc agtgaaattt tcctgggata aaacctgact gctgttttcc agcttgtcta
     9241 ttgtagattg tatttcggca atttgtctgt ttactacagc cgaatcagca tccgtattta
     9301 tatctaagag aagctgtcct tcctttattt tacttccttc ctcatgataa tatattacac
     9361 tgccatcaat ggctgcattg tatacttttt catctttgac aacaatcccc tcagccttaa
     9421 tgacatcctc caaaattccg ttttccgcta ccatgaagtc tgcaggagat ttgaaatttt
     9481 gaaatatata aatggctata atgactatta caaaagagag aaagaaattt tttaacccaa
     9541 aattttttct tgccactatt aatctccaaa tttctttata ttaaattttc cttatgaata
     9601 gtaaagaccg aggcctttgc ttcgattttt gattcatata ataattttaa gcctaaaggg
     9661 cttgttcgca ttgatagggt cattataaca taatcttcat attttttgca agtaatatta
     9721 ggtgattttc aataaaattt gcattaattt tgaaataatt attaaaatta ttcaatatat
     9781 atgacacatc taatattatt aggcaaaaaa atagattttc atatttcgtt tacatcgaaa
     9841 tagaaaatct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tatactatca
     9961 ggaatattat gcttccctgg ccgtcgttaa ctattttttc aatagtcttt ttaagctttt
    10021 tccgtgcatt gtcgggcatt gcagtaagct tggaatccag ctgttcctct accaggcttg
    10081 aaagtggttt gccgaacatc tcagcctgcc atatgctttg aggattttcc ttcatttggc
    10141 cgatgagata gcttattagg tcttcacttt gagccttgct tcctattacc ggtgatacct
    10201 ccgcattcac acctgcactg aatatatgca atgagggtgc ctgtgccttt atttttattc
    10261 catacctgcc gccttccttg aaaacttcag gagattcgat aatcatatct gcaacggtag
    10321 gggttacata tccgtagccg ctggctttag cttcatttat ggctgcttca accctgctgt
    10381 agctctgtct tgtttttgac aaatctccga taagtttaag tatctgatgc tcacctgtaa
    10441 tttcttcccc tgaaagctcg ctaattatct tgtaatagta ggaattatcc aggtccacag
    10501 ctaaatttat ttttcctgtt cccagttcaa tatctttaac atagtagtca tcaacataat
    10561 cattttcttc cgagaatatg tcttcagcat taaaatcgct taatttgtaa tagttttgga
    10621 aatcatccct aagtatgttg attaaatctt ttttcatgga ataatcatat ggcaggccgt
    10681 caaaccattt tggaaggtcc acattgattt ccttgatagg aaattcataa aggacatttt
    10741 ccagcacatc ctcaatatca tcggttttta aattcataca gtcaacaata agaacaggag
    10801 catcatattt ttccttcaat tgagcagata gagcaactgt attttcattg tttggatgag
    10861 tagtatttaa aataataaca aaaggcttgt taatttcttt cagctccctt acaactcttt
    10921 cttctgccat tatataattg tccctgttta tatcagttat gcttccgtct gttgttacca
    10981 gtactccaat tgtggaatgg tcattaatta ctttttttgt cccaatttca gctgcttctg
    11041 caaagggtat gggctcttcg ttccagggag tacttacaag tctcggtatt tcaccttcca
    11101 tatttccaat agctccggga atcatatacc ctacacaatc aataagtctt aattttgctt
    11161 taatgttatt attgaatgta acagcaaccg cttctccggg cacaaatttc ggttctgtag
    11221 tcattacagt cttgcctgta ccgctttgag gcatctcatc cttagccctt tccttagtat
    11281 attcgtctgc aatattgggc agcatcacgc tttctgaaaa ctttcttatg aatgttgatt
    11341 ttccggttct caccggtcct acaaccccca ggtatatttc acccttggtt cttttggcta
    11401 tatcctgata tatatctata ttagtgataa taatccctcc ccctgcaaat atgcactatc
    11461 gtatacaatt aatttatatg ccttgttcaa gactttattc caaaaaaaca aaaaaagaat
    11521 atattctaat agtatatatt cttttgcggg acagtttagg accgtccctg ctgcctcatc
    11581 cccgctgtcc cgttagagct tttcttcttt tttatccctg agcattaatt tatttactgt
    11641 ttcttttaca tcagccttgt tttccaagat atcatacatg gcatccacta ttggcatatc
    11701 cacgtttaat ttttttgata accaataggc tgcatatgta gtaggaattc cttcaacaac
    11761 cattcctact ttttttatcg cttcatcctt gcttaagcct tgaccgataa ggtagcctgc
    11821 attccagttt cttgaatgcc tgcttgtaca tgtgactatc aggtcaccta tgcccgtaag
    11881 tccgtaaaag gtatgaatat cagcccccat ggctattcca agccttgcta tttcaacaat
    11941 tcccctgttc ataagagctg cttttgcatt gtctccgtag cctactccgt cagatattcc
    12001 cgcgcccaga gctataatat ttttaagtgc tccgccaagc tccgctcctt taacatcttc
    12061 atttgtgtac accctgaaat attcagacat gaatatnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnaaaa ccatgacacc tatttttttc ccgaatactc ttatggcatt
    12241 tgttgtgcct gcattgccgc tgccataatc cctgacatcc ttttttacaa atatttttcc
    12301 taatatgtag gcaaatgaaa tgtttccaat aaaataagca attaccgcta ataaaaagta
    12361 tttcatgatt cctccgctac tttttcattc cgtattttca ctttggataa ggatgacaga
    12421 aagtaattat aaattctttt cgcttctgtt cctgaattca ataataatcg gcgtaccctt
    12481 gtattcaaat gcttcccgga ttttattctc aatatatctt tcatatgaaa agtgaactat
    12541 ttccttatca tttacaaata ttaaaaactt gggaggattt actgcaacct gggttccgta
    12601 aaatatttta agtcttttcc cctttactgt cgcgggctga ttcataagaa ctgcttctgt
    12661 caggatgtca tttaatactc ccgtggaaat tcttaaattt ctaaagctgt aaacctcatc
    12721 cacagcatca agtattttgt tcattctctg gcctgtaagg gctgacacgg ttattatggg
    12781 tgcatatgag gcgtatgcca gcttttgtct gatatcatct gtgaattcct tgaaggtttt
    12841 attgtccttt tcaataagat cccacttatt gactgctata atcatgcctt tactgttgtc
    12901 atgggaatat cctgcaatcc ttatgtcctg ttcagtcacc cctttatcgg catcaattac
    12961 caaaatgcat atgtcagctc tttcaaccgc tgtaaaagct ctcagcacac tgtatttttc
    13021 aacggattct aaaatcttgt tcttttttct tatgcctgca gtatcaataa gggtgtattt
    13081 tttggagttt ctttcaaact tcgtatctat ggcatccctg gttgttcccg gaatatcgct
    13141 tacaataacc ctgtcttccc ctattatttt atttattatt gaagattttc ctgcattggg
    13201 ctttcctatg acggcaattt ttacactgtc atccccctct tcgacattca tcctttcatc
    13261 aaattttgaa attatttcat ccagcaggtc acctattccc aaagcctcgg cagacgatat
    13321 ggaaacggga tctccaagac ccaggttgta aaattcatat atattatctt catttacaaa
    13381 tctgtccatt ttatttacaa caagaattat ttccttgtct gccttcctga gcatctgagc
    13441 cacttctgtg tcgtcagcag taaggccttc cttaccgtca accataaaca gaaccacatc
    13501 tgccatatct atggctattt cagcctggat acgcatttgt tcaaatattg tatccgtggt
    13561 attaggttca attcctcctg tatctatcaa tgtaaagtga ttatcaagcc actcacattc
    13621 agcataaatt ctgtctcttg taacaccggg agtatcttcg gtaatagata ttctcctgcc
    13681 tgcaattctg ttaaaaagtg ttgatttgcc cacattgggc ctgccaacta tggttactac
    13741 tggtcttccc atttttccct ccgtttattt aacaattaaa ccaagcaaag aatctgtctc
    13801 tacatcgcaa atttctatcc tggcgtttaa ttccctttcc aaatcctcaa ttgctacatc
    13861 atccagcatt acattattgt cattaagtat attccccggc agaataagat ttttataatt
    13921 ctttccctta agctggtcta ttatgtcctt tccggtaata agacctgata cggttatttt
    13981 gtctccgaaa taattattta taatttttac aacattaatc ttcacattta tcctgctcat
    14041 aattttattg gctgtatcaa ccattaaatc ataggcagca gctcctgtta caaaagtagc
    14101 ctcttccttc agcacttttt catagcataa atttttaata tcttcatcaa tactgctaat
    14161 aaaaagcctg cacatgccta ttccgttttc cagctgatca aaatcttcat attcttcata
    14221 tgaaggaaat ccattcccgc ttagaatata aaattcatct gcaatgaaaa caaatcttgt
    14281 acccagcttt ctaagcagcc tttcctgtat attttcaacc tgtcttacaa cctcttgtgc
    14341 actttctttg tcgaaaggct taaggtctgc aagcttctcc cggtgtctgg taaggcccac
    14401 aggaacaacc gcaacactcc taaggcccgg atgcagtgaa gataaatccg taatggtttt
    14461 agtgagccgg tccttatcgt ttacatccct gaccagtaca atctggcaat caaccgttat
    14521 atgggcatca acaagtcttt tcaagtattt aagtatatcc atgtccttgt cattgcccat
    14581 catatatttt ctaagctcaa ggtcggtagt gtggactgat acctttatgg gactgatcct
    14641 atacttgata atcctgtcta tttcatcatc ggataaattt gtgagagtta tgaaattgcc
    14701 gtagagaaaa gacagcctgg tgtcatcgtc cttcaaata
//
LOCUS       DAOPUT010000041        14108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_4413_length_14108_cov_19.784103, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000041 DAOPUT010000000
VERSION     DAOPUT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14108
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4413_length_14108_cov_19.784103"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKK75_05095"
     CDS             <1..528
                     /locus_tag="PKK75_05095"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNZ83007.1"
                     /translation="YLSEIKKEDMEHIYSWFSDTEFLKYYDYYPPLPLPKAEVDKMFK
                     YYEDSGKSKVFAVKKDRLTIGLAGFDDIIKENQAATLFIGLGSKNERGRGYGRKSMDL
                     LLEYGFNNLNFHRIQLNVLEFNYGAIALYEKCGFKKEGIFREFVLRDGKRYNLLLYGL
                     LKYEWEMNKEKIFRA"
     gene            complement(575..904)
                     /gene="trxA"
                     /locus_tag="PKK75_05100"
     CDS             complement(575..904)
                     /gene="trxA"
                     /locus_tag="PKK75_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNZ83008.1"
                     /translation="MAEKIITINSTEEFDSTIMKTRYVLAEFYADWCAPCRMVAPVIE
                     RIAEQYSSRITTVKVNIDEQQELAIRYGIQTIPTIIFFKEGRFASKEIGVKPLSSFVR
                     MIDSNIA"
     gene            complement(906..1763)
                     /locus_tag="PKK75_05105"
     CDS             complement(906..1763)
                     /locus_tag="PKK75_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ83009.1"
                     /translation="MGEIVDIIIIGAGPAGLSAAVNAVTRGKSVRIFSNRENYLSKAE
                     RVDNHLGFYHASGKELMDKFKEHAEAMDIKIEEGKAVNILPLGESFMVNINGEILESK
                     KVILAMGISKIKELPGEGRLLGSGVSYCATCDGMLYRGKNALVWDQSNDAWEEANFLQ
                     SIGVNVSFISQKPKNENLSNDIRFIKGTITEIIGENTVEAVNTGSETIKTDAVFMLRD
                     AVAPAALIDGLELEGSFINTSKYMETNIPGLYAAGDITGKPLQLSKAISEGLIAAQHA
                     AMQIDKKEN"
     gene            complement(1814..2545)
                     /locus_tag="PKK75_05110"
     CDS             complement(1814..2545)
                     /locus_tag="PKK75_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNZ83010.1"
                     /translation="MSSAELTANVSTMLVFLEGFISFFSPCVLPLIPVYMSYLAGNGK
                     RVNSDGTITYERKKVLFNTLFFVLGISSVFFILGLSFSALGIFFNKNRDIFSKIGGAI
                     IVILGLVQLDIIKLNFLKMEKRINMDIGGKMNPILAYIMGFTFSFAWTPCVGPALASV
                     LILASNAENAFAGNMLVFVYALGFIIPFIILGIFTTETLNFFKEKRNLVKYTIKLGGF
                     ILVIIGIMTFTGTFTAISRRLSFFK"
     assembly_gap    2653..2752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2753..5574)
                     /locus_tag="PKK75_05115"
     CDS             complement(<2753..5574)
                     /locus_tag="PKK75_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNZ83011.1"
                     /translation="MKRDNMNKGRLFVIGVIILVFLVINSFTGIVEFVTNYLWFKEMG
                     YTETFFTKIKAQISIGVPIFLILTVFLYIFIKRLKKKYDEESEIVSVNKKAGLIIKLI
                     SAGVSLFISLNITSTMWFQILQYFNSMDFGEADPVFNNDVSFYAFKLPLINTIVGSII
                     SILFLLTIVIVLFNGYLAMKDSLKKLNEEFQDIQQFPRQHLNLNNILNKKFAERIINQ
                     LSIIGVFLFLLLGARYVLRSYDLLYSRLGRVFGAGYTDINITLNLYRVLAVGCVVAAV
                     TFFTGARKRNLKTALAVPAALIIISIIGTGAAGGVEKFVVEPDQLSKETLYMQYSIKS
                     TQNAYALNDVKTIQFPANNNLTIEDLENNPEVMDNIRINDQEPLIQVYNQLQGIRPYY
                     VFNDVDVDRYVIDGVYKQVFLSARELDQGRLNEQARTWVNQYFKYTHGYGITVSTVNN
                     VTPQGQPEMLVKNIPPITETDFNIQRPEIYFGEKTNNYIIVNTDEMEFDYPSGADNVE
                     TFYEGEAGINLSFFNKLLFSIREGSYRMLISNNIDKDSRILINRNIIDRVRKLAPYLY
                     FDPNSYIVVNQDDGKLYWIIEGFTVSDKYPYSQPTGIFIEGWEVNYIRNSVKVVIDAY
                     NGTTDFYIADESDPIINVYDKIFTDLLKPISEMPEGLRKHVRYSQGFFDVQSDMYRLY
                     HIENPTVFFGREDYWDLANEKYMDGGEGPAGSSYLMFKLPGEDGVEFLLTNQYTPQNK
                     DNMIALLAARNDGENYGQLVLYEFPKTKVISGPNMIETKIDQDTNISSQLTLWSQMGS
                     DVLRGNTVVIPIEESLLYVEPIYLRSDTDSNFPEMKMVVVSYGDKILMEPTLDTAIER
                     LFGISQQKPGIPQEPETEYDDTNINDLINKANQVFTDANKASKEGNWAEYGRKIDELE
                     RILNQLNALTNNGQEESTDGGQQQEAA"
     gene            complement(5587..6348)
                     /locus_tag="PKK75_05120"
     CDS             complement(5587..6348)
                     /locus_tag="PKK75_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HNZ83012.1"
                     /translation="MNEFGKNSKSIKNHDLFLIYHETKDIEIRNQIFEKYKYMAEIIS
                     RKYSNRGIEHDDIYQIACMGLIFAIERFDITKGFEFTSFATPTILGEIKKYFRDKGWA
                     VKVPRKIQEISRKVNEYNNILASSLHRTPTIREIAEYANSTEEEVLEAFEAGKMFNSQ
                     SLDEKFDLSSDENDISLLDVMGAEDKHFSRIENKDFINKSMGKLNGLEKDIITKRFFE
                     NKTQSDIAKELNISQMTVSRIEKKSLEKLRIEYYK"
     gene            complement(6350..6724)
                     /locus_tag="PKK75_05125"
     CDS             complement(6350..6724)
                     /locus_tag="PKK75_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83013.1"
                     /translation="MDKVLLRIPKKSEYMSTIRLTSSALSNLEGFNIDEIEDIKVVIS
                     EICTFFINNLKNNSDDFQVNYEIFPDKLVVTVKDLNEGRLSGDCLKDNMSIMIIESLS
                     DTYNYDLNNKAITFEKSKAGID"
     gene            complement(6729..7040)
                     /locus_tag="PKK75_05130"
     CDS             complement(6729..7040)
                     /locus_tag="PKK75_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNZ83014.1"
                     /translation="MSLEIENKIQDNIIEIKPIGEVDIYTSPKLKNKIFALIDERNTN
                     ILIDGEGLEYIDSTGLGVLMSIYKKLQEKGLNFTIINLKPNIYKLFDITGLNKVFNIR
                     E"
     gene            complement(7055..9508)
                     /gene="gyrA"
                     /locus_tag="PKK75_05135"
     CDS             complement(7055..9508)
                     /gene="gyrA"
                     /locus_tag="PKK75_05135"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNZ83015.1"
                     /translation="MSENENKSKIIDVKITEEMQKSYLDYAMTVIVSRALPDVRDGLK
                     PVHRRILYSASELGLSPDKPHKKSARIVGDVLGKYHPHGDTAVYDAMVRLAQSFSMRY
                     PLIDGHGNFGSVDGDQAAAMRYTEARMSKMAMEMIRDFNKDTVDFRANFDETLKEPVV
                     LPSRFPNLLVNGSSGIAVGMASSIPPHNLGEVINGTIAYIENPDIDIPGLMRYIKGPD
                     FPTGAIIEGKDNIKNAYMTGRGLVKVKAKVEIEEMKNNKSRILVSELPYQVNKANLIE
                     KIAELVKDKRIEGISDLRDESDRTGMRIVIEIKRDFNPNIVLNHLYKHTQMQDSFSVI
                     MIALVNNEPKVLNLKEMIYHYVDHQRDVIVRRTKYDLNKAEARAHIVEGLLIAIDNID
                     EVIKIIRASYDDAELKLMEAFQLSEIQARAIVDMRLRRLQGLEREKLKDEYDELIKMI
                     NRFKEILASQHLIDQIIKDELKDIKNTFYDERRTEIVAEEGEINVEDLIEEENVAITL
                     TNFGYIKRLPEDTYKVQNRGGKGITALTTRDEDFVKDLFITSTHDYLMFVTNKGKIYK
                     IKAYEVPEARRQAKGTAAINLIGIESGEKIKSLIPIREFNDDEFLIFVTKKGIIKKTK
                     LSEFESNRKTGLVAIKVADDDELISVNKTDGSKDIFIVTKQGKGIRFHENDVRDMGRN
                     TMGVKAITLVDDDELVAMSIIRKSDENKHILTVTENGYAKITLSQEYRPQTRGGKGMK
                     AHNLNKKTGLIVNSLIVDKEDDIMILSTNGTIIRMHATDISIMGRDTQGVIAMRLKDD
                     DKVASIAKIFTEEDIETNE"
     gene            complement(9521..11434)
                     /gene="gyrB"
                     /locus_tag="PKK75_05140"
     CDS             complement(9521..11434)
                     /gene="gyrB"
                     /locus_tag="PKK75_05140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNZ83016.1"
                     /translation="MAADKNNNHYGADQIQVLEGLEPVRKRPGMYIGSTGERGLHQLV
                     YEVVDNSIDEALAGYCDTIMLTINEDNSIEVVDNGRGIPIETNKKTGKSTLETVLTVL
                     HAGGKFDNDAYKVSGGLHGVGVSCVNALSDYMVAEVNWNGKKYRQRYERGIPKTEVEL
                     IGKTKQTGTSITFIPDPTIFDTLEFNYTTIKHRMRELAFLNRGIKIILEDKRSKIKNE
                     FHYEGGIKEFVRYLNSKKEPLHNDVIFTEGDKDKVIVEIAIQYTDSYNENIFSFVNNI
                     NTIEGGTHLVGFKTALTRVINDYAKKAGLIKDNDVPITGEDIREGITAILSIKVTNPQ
                     FEGQTKTKLGNSEIRGIVDSITGEALNIYCEENPKTAKAIVEKSLRSARAREAARKAR
                     DLVRRKGALESMALPGKLADCSEKDPALSEICIVEGNSAGGSAKEGRDRRTQAILPLR
                     GKILNVEKSRIDRILNSEEIKNMITAFGCGIGEDFDISKLRYHKIIIMTDADVDGAHI
                     RTLLLTFFYRYMKELITGGYVYAAQPPLYRVKKGKFEKYVYSDGELNEVLDEIGRDNK
                     NEIQRYKGLGEMDPEQLWETTMNPQTRILLKINEDDAMEADEIFNVLMGDKVGPRREF
                     IEENARYVQNLDI"
     gene            complement(11446..12600)
                     /gene="recF"
                     /locus_tag="PKK75_05145"
     CDS             complement(11446..12600)
                     /gene="recF"
                     /locus_tag="PKK75_05145"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNZ83017.1"
                     /translation="MNVPKSCFMIPKHNMMVNKLIIKNYRNFKEAEAELNNSLNIFMG
                     DNGQGKTNLMEAIYIASIGRTFRLNSENELIYFCENKSSLEIHITKNNYNIKIEILLE
                     KNKKKQVFINGVKLDKTSEMIGILNNVIFTPDDLKIIKGSPVERRKFINIDISQIKPK
                     YKYLLNKYKKITNERNMILKNYHANYKNKDIICIWNDYLVNTGTEISIYRNEYINKLK
                     LYAADIYASISGNKEKLDIFYYSNIGFITEREDIKKAFYEKINKNSNNEILKGTSLYG
                     PHKDDLIIKINGKELKYFGSQGQQRSAILALKLAEIEIIKEETGEYPILLLDDVLSEL
                     DNKRKSCLINHIEGIQTIITCTDDHEISKLTKDFNKKIFYINQGRIDTLN"
     gene            complement(12645..12878)
                     /locus_tag="PKK75_05150"
     CDS             complement(12645..12878)
                     /locus_tag="PKK75_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNZ83018.1"
                     /translation="MERISINTEYIKLDQLLKFANAVEGGGMAKNVILDGLVKVNEEV
                     VLQRGKKLREGDIVEFNNKKYLIQRYGDSPSEK"
     gene            complement(12960..14078)
                     /gene="dnaN"
                     /locus_tag="PKK75_05155"
     CDS             complement(12960..14078)
                     /gene="dnaN"
                     /locus_tag="PKK75_05155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNZ83019.1"
                     /translation="MKVKINQNELNKSINIVQKAVSSRTPLPILSGILIEAKNNMLIL
                     TATDLDLGIKTYSPCEIEEEGSIVVQSRLIGDFVRKLPSNTYVFIETTENNNMEIKCL
                     NSEINILGNSASEYPDNTFDNEGESFRIKSEVLKNLIKHTYFAAALENIKPIFTGCLI
                     EIKNNLCTVVALDGYRMAVKKEEIDYTGEISVVIPSKALFEILRIIEENKEETEIVVS
                     ESHISFKVENTIIISNLLDGKFIDYEGIIKDNYVSVVKVNTGDIRDSIERASLLARDD
                     KNNLIILDIKDFNMQIFSASEYGNVEENISIDKEGEDIKIGFNSKYILDYLKVIDSEM
                     ISLNLIGKNNPCFIKEESEEKEVNKDNYIYMVLPVRIS"
BASE COUNT         3823 a         2884 c         2106 g         5195 t
ORIGIN      
        1 tatttatcag aaatcaaaaa agaagatatg gaacacattt acagttggtt ttccgacact
       61 gagtttctaa aatattatga ttattatccg cctcttcctt tacccaaggc tgaagtggat
      121 aaaatgttca aatattatga ggacagcggc aaatccaagg tctttgccgt aaaaaaagac
      181 aggttgacaa taggccttgc cggctttgat gatataataa aagaaaatca ggctgcaacc
      241 ttgtttatag gtttgggcag caaaaatgaa agaggcagag gatacggcag aaaatccatg
      301 gatttattgt tggaatatgg ttttaacaac cttaatttcc atagaattca gctgaatgtt
      361 ctggaattta attacggagc aattgcccta tatgaaaaat gcggttttaa aaaggaaggc
      421 attttcaggg aatttgtatt aagggacggc aaaagataca acctgctttt atatggcttg
      481 ttaaaatacg agtgggaaat gaataaagaa aaaatattcc gagcttaaag gcatattgag
      541 ggcacacctc tagaggtgtg ccccatacct tttgttaagc aatattgctg tcgatcattc
      601 tcacaaagga agacagtggt tttactccta tttcctttga tgcgaacctt ccttccttaa
      661 agaatattat agtaggtatt gtttgtatac catagcgaat agccaattcc tgctgctcat
      721 ctatatttac ttttacagtt gtaattctac tgctgtattg ttcagcaatc ctttctatta
      781 ccggtgcaac cattctgcag ggagcacacc aatctgcata gaattccgcc aaaacatatc
      841 tggttttcat tattgtgctg tcaaattcct cagtactgtt aattgtaata attttttctg
      901 ccatttcaat tctccttttt atctatttgc attgctgcat gctgggcagc aatcaaacct
      961 tcgcttattg cttttgaaag ctgaaggggt tttcctgtaa tatcccctgc cgcatataga
     1021 cccggaatgt ttgtttccat gtatttgctg gtatttatga agctgccttc caattccagt
     1081 ccgtcgatca atgcagcagg tgcaacagca tccctgagca taaacacagc gtctgttttt
     1141 atggtttcac tgcccgtatt aacagcttca actgtgtttt cgcctattat ttcagtaata
     1201 gtacctttaa taaatcttat atcattgctt aagttctcgt tttttggttt ttgagatata
     1261 aagctgacat tcaccccaat gctctgcagg aagtttgctt cttcccaagc gtcattggac
     1321 tggtcccata caagagcatt cttgcctctg taaagcattc catcgcatgt ggcgcagtag
     1381 cttactccgc ttcctaaaag ccttccttct cccggaagtt ctttaatttt tgatattccc
     1441 attgcaagta ttactttttt tgattctaaa atttcaccgt ttatgtttac cataaaactt
     1501 tcgcctaagg gaagtatgtt cactgctttg ccttcttcga tttttatatc catagcttct
     1561 gcatgttctt taaacttatc catcaattct tttccgcttg catgataaaa acccaaatga
     1621 ttatcaaccc gctcagcttt tgacagataa ttttccctgt ttgaaaaaat tcgtacactc
     1681 tttccacggg taacagcgtt taccgctgct gacaagcctg caggacctgc tcctataatt
     1741 attatatcta ctatttctcc catattaacc tcacattctt cattgattca cccggcggct
     1801 tactccggaa taattactta aaaaaactca atcttctgct tatggcagta aaggttcccg
     1861 taaaagtcat aatcccgatt attactaaaa taaaaccgcc cagttttatt gtatatttga
     1921 ctaaattcct tttttcctta aagaaattca atgtttctgt ggtaaatatc cccagaatta
     1981 taaagggtat tataaatccc aaggcataaa caaatacaag catatttccc gcaaatgcat
     2041 tttcagcatt tgatgccagt attaatactg atgccaatgc aggtcccaca cagggagtcc
     2101 atgcaaagct gaatgtaaat cccataatgt atgcaagtat gggattcatt tttccgccta
     2161 tatccatatt tatccttttt tccattttta aaaaattcag ttttatgata tcaagctgta
     2221 caagacccag gatcactatt attgcgccgc ctattttgct gaatatatct ctgttcttat
     2281 taaagaatat acccaaagct gaaaatgata aacctaaaat aaaaaataca ctggaaatac
     2341 ccagcacaaa aaataaggtg ttaaaaagaa ccttttttct ttcatatgta atagtgccgt
     2401 cactgtttac tctttttccg tttcctgcca aatagctcat atacacaggt attaacggaa
     2461 ggacacatgg tgaaaaaaat gatatgaacc cttccaaaaa aaccagcata gtgcttacat
     2521 tggctgttaa ttccgcactg ctcattttag ccccccggga atattttatt aaaatatagc
     2581 agtgtaattt tactttgtca agtaacattg tcacaataat atttataagc cgcaggctta
     2641 cctgcggctt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcagcttc
     2761 ctgctgctgc ccgccgtcgg ttgattcttc ctggccgtta tttgtcagag cattaagctg
     2821 atttaaaatt ctttccaatt catcaatttt tctgccgtat tctgcccagt taccttcttt
     2881 tgaagcttta tttgcatcgg tgaagacctg attggcctta ttgattaagt catttatatt
     2941 tgtatcgtca tactctgttt caggttcttg aggtattccg ggtttttgct gtgatatgcc
     3001 gaaaagtctt tctatagctg tgtctaatgt tggctccatc aatattttat caccatagga
     3061 tacaacaacc attttcattt cgggaaagtt gctgtcggta tccgatctta aatatatggg
     3121 ctctacatac agcaatgatt cctctatggg gattaccaca gtgtttcccc ttaaaacatc
     3181 cgaacccatc tggctccaca gtgtaagctg tgaagatata ttagtatcct ggtcaatttt
     3241 tgtttctatc atattaggtc cggaaattac ttttgtttta gggaattcat acagcacaag
     3301 ctggccgtaa ttttctccgt cattccgggc tgcaagaagt gctatcatat tgtccttgtt
     3361 ctggggagta tactgattgg tcaataaaaa ttccaccccg tcctcgcctg gaagtttaaa
     3421 catcagatag cttgagcctg caggaccttc tccaccgtcc atgtattttt catttgcaag
     3481 gtcccagtaa tcctccctgc cgaagaaaac tgttggattt tcaatatggt acaatctgta
     3541 catatctgac tgtacatcga aaaatccctg ggagtatctc acatgttttc tcaaaccttc
     3601 aggcatttca cttattggtt ttaacaaatc agtaaaaatc ttatcatata cattaataat
     3661 aggatcgctt tcatctgcta tataaaaatc tgttgtgcca ttatatgcat ctataacaac
     3721 ctttactgaa tttctgatat agttaacttc ccatccctct ataaatatac ctgtaggctg
     3781 ggaataagga tatttgtcgc ttacggtaaa tccttcaata atccaataca atttgccgtc
     3841 atcctggttt actacaatat acgaattggg gtcaaaataa agatatgggg ctaattttct
     3901 taccctgtca attatattcc tgttgataag tattcttgag tccttgtcaa tattgtttga
     3961 aatcagcatt ctatagctgc cttcccttat gctgaacaaa agtttgttga aaaaagaaag
     4021 attgattcct gcttctccct cataaaatgt ttcaacattg tctgcacctg aagggtagtc
     4081 aaattccatt tcatctgtat ttacaattat ataattattt gttttttcac caaagtaaat
     4141 ttcaggtctt tggatgttaa agtctgtttc agttatggga ggaatatttt taacaagcat
     4201 ttccggctga ccctgaggtg tcacattatt tacggtagat actgttattc catagccgtg
     4261 ggtgtattta aaatattgat tgacccaggt tcttgcctgt tcgtttaatc tgccctggtc
     4321 aagttccctg gctgaaagga aaacttgctt gtaaacaccg tcaattacat atctgtccac
     4381 atctacatca ttaaaaacat aatagggtct tatgccctga agctgattat aaacctggat
     4441 taaaggctcc tggtcgttta ttcttatatt gtccattact tcaggattgt tctccaaatc
     4501 ctcaattgtc aaattattat tggctggaaa ttgtattgtt ttcacatcat ttaacgcata
     4561 tgcattttgg gtgcttttta tactgtattg catatataat gtttcttttg acagctggtc
     4621 aggctcaact acgaattttt ctaccccgcc tgccgcacct gttccgataa ttgatattat
     4681 gataagggct gcaggaactg ccaatgctgt tttcagattt ctttttcttg cacctgtaaa
     4741 aaatgtcact gctgcaacaa cacatccaac agccaggacc ctgtataaat tcaaggtaat
     4801 attaatatcc gtatatcctg ccccaaatac cctgcctaac cgggaataaa gcaaatcata
     4861 gcttcttaat acatatctgg cacccagaag caggaacagg aaaacgccaa tgattgaaag
     4921 ctggtttatt attctttctg caaacttttt atttaaaata ttatttaaat tcagatgctg
     4981 acggggaaat tgctgtatgt cctgaaattc ctcattaagc ttttttaaac tgtccttcat
     5041 tgcaagataa ccgttaaaca aaactataac tattgtcagc aaaaatagta tggaaataat
     5101 agaccctaca attgtattta tcaggggaag tttgaaagca taaaaactca catcgttatt
     5161 aaatacaggg tcagcttctc caaagtccat actgttgaaa tattggagta tttgaaacca
     5221 cattgtggat gttatattca agcttataaa aagactgact cctgcagaaa ttagtttaat
     5281 aatcaaaccc gcttttttgt ttacactgac aatttctgat tcttcatcgt attttttctt
     5341 caatctcttg ataaatatat acaagaaaac agtcagtata agaaaaattg gaactccaat
     5401 tgatatttgt gctttgattt ttgtaaagaa agtttctgta tagcccatct ctttgaacca
     5461 caaataattt gtaacaaatt caacaatgcc ggtaaaggaa ttaatcacaa gaaatacaag
     5521 gataataacc ccaattacaa acagtcttcc cttattcata ttatctcttt tcacatatta
     5581 cctcccttat ttgtaatatt cgattcttaa tttttccaaa gacttctttt ctattctgga
     5641 tactgtcatt tgggatatat ttaattcttt ggcgatatca ctttgggttt tgttttcaaa
     5701 aaaccttttt gtaattatat ctttttccag accgttcaat ttacccatgg atttatttat
     5761 gaaatctttg ttctcaatcc ggctgaaatg cttatcttca gctcccatca catccaggag
     5821 ggatatgtca ttttcatcac tgctcaggtc gaatttttca tccaatgatt gagaattaaa
     5881 catttttcct gcctcaaagg cctctaaaac ttcttcctct gtagagtttg cgtattcggc
     5941 aatttccctt atggtgggtg tcctgtgcag ggagcttgct aaaatattgt tgtattcatt
     6001 gacttttctt gatatttcct gaatttttct gggcacctta acagcccagc ccttatccct
     6061 gaaatatttt tttatttctc ccaatattgt tggggtagca aagctggtaa attcaaatcc
     6121 ttttgtaata tcaaatcttt caatggcaaa aataagtccc atacatgcaa tttgatatat
     6181 atcatcatgt tcaatgcctc tgttgctgta ttttcgggat atgatttcag ccatatattt
     6241 atacttttca aagatttgat ttcttatttc aatatctttg gtttcatgat aaattaaaaa
     6301 caaatcatgg ttttttatac tcttggagtt tttaccaaac tcattcattt caatcaattc
     6361 ctgctttaga cttttcaaaa gtaatagctt tattatttaa atcataatta taggtatcag
     6421 acaagctttc tataatcatt atgctcatat tatcctttaa acaatcaccg gataatcggc
     6481 cttcatttaa gtctttaact gtcacaacaa gtttatcggg gaaaatttcg taatttacct
     6541 ggaaatcatc cgagttattt ttgagattat taatgaaaaa ggtgcagatt tcagaaatta
     6601 ccacctttat gtcctcaatc tcgtcaatat tgaatccttc caaattggaa agagcggatg
     6661 atgttaatct tattgtactc atatattcgg atttttttgg aattcttaat aatactttat
     6721 ccatttcgtc actcccggat attgaatact ttatttaaac ctgtaatgtc gaaaagtttg
     6781 taaatattag gtttcaagtt tattatcgta aaattcagcc ctttttcctg cagcttttta
     6841 taaatgctca tcaagacgcc aagccccgta ctgtcaatat actccagacc ttcaccatca
     6901 ataaggatat ttgtatttct ttcatcaatc aacgcgaata ttttattttt cagtttggga
     6961 gaagtataaa tatctacttc tcctattggt tttatttcta tgatgttatc ctgtattttg
     7021 ttttcaattt ccaatgacat agtttttacc tccactattc atttgtttca atatcttcct
     7081 ctgtaaaaat ctttgctatt gacgcaacct tatcgtcatc tttaagtctc atggctataa
     7141 caccctgagt atctcttccc attatggaaa tatccgttgc atgcattctg attatagttc
     7201 cgttagtgct taatatcata atgtcatctt ctttatcgac tatcaatgaa tttacaatta
     7261 gaccagtctt tttgttcagg ttgtgggcct tcattccttt tccacccctg gtctgaggtc
     7321 tgtattcctg ggacagggtt atttttgcat agccgttttc tgtaactgtt aatatatgct
     7381 tgttttcatc gcttttcctg atgatgctca ttgcaacaag ttcatcgtca tcaacaaggg
     7441 ttattgcctt aactcccatt gtgtttctgc ccatatcccg tacgtcgttt tcatggaatc
     7501 ttataccttt gccttgtttt gtaacaatga atatatcctt gctgccgtct gtcttgttca
     7561 cactgataag ttcatcatcg tctgctactt ttatagccac cagaccggtt tttctgtttg
     7621 attcaaattc gctgagcttg gtttttttga taattccttt cttggttaca aatatcaaaa
     7681 attcatcgtc attaaattcc cttatgggaa tcaatgattt tattttttca ccggactcaa
     7741 tgcctattaa gtttattgca gctgttcctt tagcctgacg ccttgcttca ggtacttcat
     7801 acgctttgat tttataaatt ttacccttgt ttgtaacaaa catcagataa tcatgagttg
     7861 atgttataaa caaatccttt acaaaatcct catctctggt tgtaagtgcg gttattccct
     7921 ttccgcccct gttttgaacc ttgtaggtat cctccggaag cctttttata tagccgaaat
     7981 tcgtcaatgt aatcgcaaca ttttcttcct ctattaaatc ctctacattg atttctcctt
     8041 cttcggctac aatttctgtt cttctttcat catagaaagt gttttttata tcctttaatt
     8101 catccttgat tatctggtca atcaagtgct gacttgctaa aatttcctta aacctgttta
     8161 tcattttaat aagctcgtca tattcatcct ttaatttttc tctttcaagc ccctgaagac
     8221 gtctaagtct catatcaact atggctcttg cctgaatttc tgacagctgg aatgcttcca
     8281 tcaattttag ctcggcatca tcataggatg ctcttataat cttaataacc tcgtcaatat
     8341 tatcaattgc aatcaaaagc ccctcaacaa tatgagccct tgcctcagct ttgtttaaat
     8401 catattttgt ccttctgact ataacatccc tctgatggtc tacatagtga taaatcattt
     8461 ctttcaggtt taaaaccttg ggctcattat ttaccaaggc aatcattatt acgctgaaac
     8521 tgtcctgcat ctgagtatgc ttataaagat ggttcagcac aatgttggga ttgaaatctc
     8581 ttttaatttc aataacaatc ctcatgcctg tcctgtcgct ttcatctctt aagtctgaaa
     8641 ttccttcaat tcttttatcc tttacaagct cggcaatttt ttcaattaga tttgccttat
     8701 taacctgata gggcagttct gatactaata tccggctttt gttatttttc atttcttcta
     8761 tttcaacttt agccttaacc tttacgagtc cccgacctgt catataagca ttcttaatat
     8821 tgtccttacc ttcaattatg gctcctgtgg gaaaatctgg accttttatg tatctcatga
     8881 gaccgggaat atcaatatca ggattttcaa tatatgcaat agttccgttt attacctccc
     8941 ccagattgtg aggaggtatg gagcttgcca ttcctacagc tatgcctgaa gacccgttga
     9001 caagcagatt ggggaaacgg cttggaagca caaccggctc ttttagggtt tcatcaaaat
     9061 tcgccctgaa atcaacagta tctttattga aatcacgaat catctccatt gccatctttg
     9121 acatgcgtgc ttctgtgtaa cgcattgccg ctgcctggtc gccgtctact gaaccgaagt
     9181 tgccgtgccc gtctatcaaa ggataacgca ttgagaaact ctgggctaac cttaccatgg
     9241 catcatatac ggctgtgtct ccgtggggat gatacttacc taaaacgtct cccactatac
     9301 gggcgctttt tttatgtggt ttatcagggg aaagacccag ttcacttgct gaatacaaga
     9361 ttcttctatg aacaggcttt aagccgtctc ttacatcagg cagtgccctt gacacgatta
     9421 cggtcatggc atagtccaaa taagactttt gcatctcttc agtgattttt acgtctataa
     9481 ttttgctttt gttctcattt tcgctcattt gcaacctctt ttatatgtct aaattctgta
     9541 catacctggc attttcttca ataaattctc ttctgggacc aaccttgtct cccattaaaa
     9601 cgttgaatat ttcatctgct tccatagcat catcctcatt gatttttaaa aggattctgg
     9661 tttggggatt catggttgtt tcccaaagct gttccggatc catttctccg agacctttgt
     9721 acctttgtat ttcgttcttg ttatctcttc cgatttcatc caaaacctca ttaagctccc
     9781 cgtcgctgta cacatatttt tcaaattttc ctttttttac tctgtataaa ggaggctggg
     9841 cagcataaac atatcctccc gttattagct ccttcatata cctgtagaaa aatgtgagaa
     9901 gcaaagtcct tatatgggct ccgtcaacat cggcatctgt cataattata attttatgat
     9961 atcttaattt agaaatatca aaatcctctc caattccgca gccaaaggca gtaatcatat
    10021 ttttaatttc ttcagagtta agtattctgt ctatccttga cttttcgaca ttaagaattt
    10081 tacccctcaa tggaagtatt gcctgtgttc tcctgtctct tccttccttg gcagaacctc
    10141 ctgcagaatt accctcaaca atacagattt cactgagtgc cgggtccttt tccgaacagt
    10201 ctgccaattt gccgggcagg gccatacttt caagagcacc ctttcttctt accaggtctc
    10261 ttgcttttct ggctgcttct cttgctcttg cagatcttaa tgatttttca actattgctt
    10321 ttgctgtttt aggattttcc tcacagtata tgtttaaagc ctcaccggtt atgctgtcaa
    10381 ctattcccct tatttcgctg tttccaagct tagtcttggt ctgaccttcg aactgcggat
    10441 ttgtaacttt tatcgacagt attgcagtaa ttccttccct tatatcttca cctgtgattg
    10501 gcacatcgtt atcctttatt aaacctgcct tttttgcata atcatttata acacgtgtaa
    10561 gggcagtttt aaatccgact aaatgagttc ctccctcaat tgtattaata ttgtttacaa
    10621 atgaaaaaat attttcattg taggaatccg tatactggat tgcaatttct acaattacct
    10681 tatccttatc tccttcagta aatataacat cgttatgaag aggctctttc ttgctgttta
    10741 aatatcttac aaactccttt attccccctt cataatgaaa ctcattttta attttgcttc
    10801 ttttatcttc aaggattatt tttattcccc tatttagaaa tgccagctct ctcattctgt
    10861 gctttatagt agtgtaatta aactccaaag tatcaaaaat tgttggatct ggaataaatg
    10921 taatgcttgt tcctgtttgt tttgtttttc ctattaattc aacctctgtc tttggaatgc
    10981 ctctttcgta tctttgcctg tactttttac cgttccagtt tacttccgcc accatatagt
    11041 ctgatagggc atttacgcat gaaacaccga caccgtgaag gcctcctgat actttatatg
    11101 catcgttgtc aaatttaccc cctgcatgaa gaacagtcaa aacagtttcc aatgttgact
    11161 tacctgtttt tttatttgtt tcaataggaa tccctcttcc attgtctaca acctcaattg
    11221 agttgtcttc atttatggtt aacataatgg tatcgcagta tcctgccaat gcttcatcta
    11281 tgctgttgtc tactacttca taaaccagct ggtggagccc cctttcccct gtagagccaa
    11341 tatacattcc cggtcttttc ctgacaggct ccagaccttc aagaacttga atctgatctg
    11401 cgccgtaatg attattattt ttatctgccg ccatttattt ctcctctaat ttaatgtatc
    11461 tattctgcct tggtttatat aaaaaatctt tttattgaaa tccttcgtta atttactaat
    11521 ttcatggtca tcagtacatg ttattattgt ctgtattcct tcaatgtgat taatgagaca
    11581 gctttttctc ttattgtcaa gctctgacaa aacatcgtct aacaataaaa taggatattc
    11641 tcctgtttct tccttgatta tttctatttc agcaagcttc aatgctaaaa ttgcagaccg
    11701 ctgctggcct tgagacccga aatactttaa ttcctttccg ttgattttta tgattaaatc
    11761 atccttgtgg ggcccataaa gagaagtgcc ttttaaaatt tcattgttgg aattcttgtt
    11821 tattttttca taaaatgctt tcttgatatc ttccctttct gtaatgaatc caatgttgct
    11881 gtagtaaaat atatccagtt tttccttgtt tcccgatatg cttgcataaa tatctgctgc
    11941 ataaagtttt aatttattta tgtattcatt tctgtaaatg gatatttccg ttcctgtatt
    12001 taccaggtag tcattccaaa tacagatgat atccttattc ttataatttg catggtagtt
    12061 ttttaaaatc atgtttcttt cattggtaat cttcttgtac ttgttgagca aatacttgta
    12121 tttgggcttt atctgcgaaa tatctatatt aatgaattta cgtctttcaa cagggcttcc
    12181 ttttataatt ttcaaatcat cgggagtaaa aataacatta tttaaaatac caatcatttc
    12241 cgatgtttta tcaagcttaa ccccattgat aaaaacttgt ttctttttat ttttttccaa
    12301 taatatttct atttttatat tgtaattgtt ttttgtaata tgaatttcaa gggagctttt
    12361 gttttcgcaa aagtatatca attcgttttc actgtttaat ctgaaagttc ttccaatgga
    12421 tgcaatataa attgcttcca taagattagt ctttccctgt ccgttgtctc ccataaaaat
    12481 attaagagaa ttattcaatt ctgcttcagc ttctttaaaa ttcctatagt tcttaattat
    12541 aagcttgttt accatcatat tatgtttggg gatcataaag catgattttg gtacattcat
    12601 ccattgacta tcccgcagag actatctgac aactttatgt gtccttattt ctctgaaggt
    12661 gagtccccat acctctgaat taaatatttt ttattattaa attcaactat atcgccttct
    12721 cttagctttt ttcccctttg cagcacaact tcttcgttaa cctttacaag tccgtcaagt
    12781 atgacatttt tagccatccc cccaccttca acagcatttg caaactttaa cagctggtca
    12841 agtttaatat attctgtatt aatgcttatt ctttccatac aatacccttt cctattcggg
    12901 gacattcctt ctactatcag agactctccc attacaggaa ggtgtgtccc catacctatt
    12961 tagctgattc tcaccggcag aaccatataa atgtagttat ccttgtttac ttctttttct
    13021 tcactttctt ccttaataaa acatggattg ttttttccaa ttaaattcaa tgaaatcatt
    13081 tcactgtcta ttacctttaa ataatcaagt atgtattttg aattaaaacc tatttttatg
    13141 tcttcccctt ctttatctat ggatatattt tcctcaacat tgccatattc agaagcggag
    13201 aaaatctgca tattgaaatc ctttatatcc aaaataatca aattgttctt gtcatctctt
    13261 gcaagaagcg atgctctttc aatgctgtct ctaatatccc cggtattgac cttcacgaca
    13321 gatacataat tatctttgat tattccttca taatctatga attttccatc cagcagattt
    13381 gaaattatta tagtgttttc aaccttaaat gagatatggc tttcagatac aacaatttct
    13441 gtctcttctt tgttttcttc aataatcctg agtatttcaa acaatgcttt tgaaggaata
    13501 actactgata tttctcctgt ataatcaatt tcttcctttt taaccgccat tctgtacccg
    13561 tcaagggcta caaccgtgca caggttattt tttatttcta taagacatcc tgtaaatatg
    13621 ggttttatgt tctctaatgc tgcagcaaaa tatgtatgtt taattaaatt cttaagcact
    13681 tctgatttaa ttctaaaaga ttccccttca ttgtcaaatg tgttgtcagg atattcagaa
    13741 gctgagttgc ccagtatatt tatttcagaa ttaaggcact ttatttccat gttgttattt
    13801 tccgtagttt caataaacac atatgtattt gatggaagct ttctgacaaa atcccctatc
    13861 agtctggact gaactacaat tgatccttct tcttcgattt cacaggggga atatgttttt
    13921 attcctaaat ccaaatccgt tgctgttaaa atcagcatat tattttttgc ttcgattagt
    13981 atccccgata atataggaag tggtgttctt gaagaaacag ccttctgtac aatattaata
    14041 gacttattaa gttcattttg gttaattttt actttcataa cagactcctt tcagttctta
    14101 attatatt
//
LOCUS       DAOPUT010000042        13855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_4510_length_13855_cov_19.534348, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000042 DAOPUT010000000
VERSION     DAOPUT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4510_length_13855_cov_19.534348"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(590..2008)
                     /gene="gatB"
                     /locus_tag="PKK75_05160"
     CDS             complement(590..2008)
                     /gene="gatB"
                     /locus_tag="PKK75_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNZ83020.1"
                     /translation="MSYELVAGLETHIELSTNTKIFCKCTTEFKGEPNTHCCPICMGM
                     PGTLPKLNKKVVEYAVKAGLATNCKIRNVSKMDRKNYVYPDLPKAYQISQFDKPICYE
                     GYIKLSSGKNININRIHIEEDAGKLIHERGDTYIDNNRGGVPLIEIVTEPDFRSIDEV
                     LEYMEKLRLIMKYIGVSDVKMQEGSLRCDVNISVREKGQEKYGTRTEIKNMNSLNFIA
                     KAMEYEFNRHVDILESGGVIEQETRRYDSDLNITESMRGKEDADDYRYFRDPDLVNIK
                     VTEEEINRLKGQLPQLPDEKMDIFMNEYGLPKDDADLLIKYKKIAEYFEEAGKDIKNK
                     KLVSNYILGQVFRRIPTDSDKEECRISIPPAYINELIYLLENKKINSSMASNALEKML
                     DTGKPVNEFISKKDMEGIDDDALKEICMRAVSGNKEAVESYLGGKEKAFGSIIGYIMK
                     ETKGKADPQKAAEIVKEIINKG"
     gene            complement(2005..3462)
                     /gene="gatA"
                     /locus_tag="PKK75_05165"
     CDS             complement(2005..3462)
                     /gene="gatA"
                     /locus_tag="PKK75_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNZ83021.1"
                     /translation="MSKIKHIQKLLENKDISCVELTKKYLTAIEKQNKIINAYVNVTA
                     DEALKTAEKVDERIRAGEKLMPLEGIPMSLKDNISTKDIETTCCSRMLKGYYPIYDAT
                     VWEILKNQNAVLLGKTNMDEFAMGSSCETSIFGGTLNPNNTDYVAGGSSGGAAASVKG
                     NMAAFGIGSDTGGSIRQPASFCGLVGLKPTYGTVSRYGVVAYASSLDQVGPITESAED
                     AAIVYDTISIYDPKDSTSTGSKTTASDKLDKSVKGLKIGIAKEYFEGINGEVKSSLEE
                     AIKAYQDAGAEIINFEMPRLHAVLPAYFIISCAEASSNLGRYDGIRYGYKTDSYKDIN
                     EMICKNRSEGFGREVQRRILLGTYALSSGRYDAYYRKAQNLRSSVIQDFKNIFKSCDV
                     ILTPSVPVTAYKKGEALADPVEIYMMDICTVPVNIAGLPAVSVPCGFDENKMPIGMQL
                     IGNSFEDYKVLNAAYQFEKLTKKTGGRSPYPEVEI"
     gene            complement(3467..3754)
                     /locus_tag="PKK75_05170"
     CDS             complement(3467..3754)
                     /locus_tag="PKK75_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HNZ83022.1"
                     /translation="MITKEEVLRIAKIAKLSVKDDEIDLLIKDMSTVIEYADLINSVV
                     EDQDNEFGDINNIYNAFNEDIVVDSYDRDEILKNREGGENGYFVVRKSLRG"
     gene            complement(3763..5511)
                     /gene="aspS"
                     /locus_tag="PKK75_05175"
     CDS             complement(3763..5511)
                     /gene="aspS"
                     /locus_tag="PKK75_05175"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNZ83023.1"
                     /translation="MLRANEYRTHTCGQLRESDIGKQVRLSGWLENVRDHGGVKFVDL
                     RDQYGIVQVVVEDEEMLEGIPRESAIRVTGLVAERDEDTINKKLDTGYIEIEADSIDV
                     LGKVREFLPFDVTTSKETKEDLRLKYRFLDLRNVEVQKNIILRSQVISFIRSKMTDMG
                     FLEIQTPILTSSSPEGARDYIIPSRKHPGKFYALPQAPQVFKQLLMVSGFDKYFQIAP
                     CFRDEDARADRSPGEFYQLDFEMSFATQEDVFSAAEEVLYQTFTKFSNREVSKPPFKR
                     IPYEEAMLVYGSDKPDLRNPLKIIDISPMFEKTDFAPFRKKTVRAINVPDCSSQPKRF
                     FEDMLGFATKIGMKGLGYIKVDDEMKYQGPIDKFLSQEDRKELAHKANLNPGDVLFFI
                     ADTKDHAPKLAGQIRTELAKRLDLIDESRYEMCFIVDFPMYEVDEETGATAFTHNPFS
                     MPQGGLKSLIEKDPLDIIAFQYDIVCNGIELSSGAVRNHDLDIMIKAFEIAGYTKDEL
                     QTKFASLFNAFKYGAPPHAGMAPGIDRIIMLLTGEENIREVIAFPMNNNAQDLMTNAP
                     CTVTEQQLREVHIKLR"
     misc_binding    complement(5829..6079)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6135..8576)
                     /locus_tag="PKK75_05180"
     CDS             complement(6135..8576)
                     /locus_tag="PKK75_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNZ83024.1"
                     /translation="MMGGLNNSAKNALALAQEEVKRFRQNVLGTEHILLGLMIEEEGI
                     AGKLLRDKLDVEKVREIIKNSTGMGDSIPEYITISPRSKYIIELARQFAAQLKTPYVG
                     SEHILLGLIEEGEGIGAQIIKSAVNLKDLKNEVLMSMHGGGGQEPVNPNMGGGMAQEN
                     SDTRTIDKYGTDLNQRAKEGRLDPVIGRENVIERVIQILSRRTKNNPCLIGEPGVGKT
                     AIAEGLAQEIIKGSVPETLKDKRVITLDMAGMVAGAKYRGEFEERLKNAIDEIRAAGN
                     IILFIDEMHTIIGAGAAEGAIDASNILKPVMARGELQVVGATTIDEYKKHIEKDAALE
                     RRFQPIIVEEPTVEDTIRILKGLRDKYEAHHKVAITDEAIDAAANLSHRYISDRFLPD
                     KAIDLIDEAASRVRLKSSTAPKELKEMEDKIKEVDTEKRTAINNQDFEKAASLRDEEK
                     KLSDELDKQKKLWAAKNIKDIKIAYEDIADVVSQWTGIPVKKLQGDESERLLNMEEIL
                     HKRVIGQEPAVEALSKAIRRSRVGLKDPKKPIGSFIFLGPTGVGKTELSKALAEAMFG
                     DEDAMIRVDMSEYMEKHSVSKMIGSPPGYVGFDDGGQLTERIRRKPYSVILFDEIEKA
                     HPDVFNILLQILDDGRLTDAKGRTVNFKNTVVIMTSNVGASTIKKQKTMGFAASSTNA
                     EKDEYEKMKENVLDELKRTFRPEFLNRIDEIIVFHSLNKDHIKEIVSLMINNLSKRLD
                     EMGIHIEVDDKAKDLLADAGFDQVFGARPLQREIRRQIEDKLSEELLKGAIQKSDTIK
                     ITADGKDLIFTNDKK"
     gene            complement(8602..9660)
                     /locus_tag="PKK75_05185"
     CDS             complement(8602..9660)
                     /locus_tag="PKK75_05185"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HNZ83025.1"
                     /translation="MNWRQNLETKFTRWKIKRGMNDLENNDIVITSRIRLARNLKNYK
                     FPIIMSEAEANCVIGEISNALSKHDKNYKLTYIKDLSDIKKNSLIENHIISPALANKK
                     DAGAFLLSPDQKISVMINEEDHIRIQTIEDGLSLRECWEISNNVDDIIEENVDYAFDN
                     ELGYITACPTNIGTGMRASVMLHLPALSITNQIDKLLYGVSQLGVAVRGVYGEGTKSI
                     GHLYQISNQGTLGVSEETLIEKINQIVMQIIEKENKTRDHLLKNNYDDLEDDFYRAYG
                     LLTNARKISSDEAMKLLSLIKLGSEMGITPMVKGKNIYRLMVWSQPNNISTIEEKELS
                     PKERDKKRAEIIRSELLS"
     gene            complement(9596..10093)
                     /locus_tag="PKK75_05190"
     CDS             complement(9596..10093)
                     /locus_tag="PKK75_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNZ83026.1"
                     /translation="MLCNECGKNEANVHLTHIINGKKTESHLCEECAKKNQAILNSNI
                     SMENLFSAMLNNTFNNKTYLPAKSCPNCGTTYENFRNTGKFGCSRCIDSFKGRLKPVI
                     KSMQGFDRHTGKIPKRAGGDYKVQMDIERLKNDLKAAVDREEYELAAELRDKIHEMEN
                     KKGDE"
     gene            complement(10112..10585)
                     /locus_tag="PKK75_05195"
     CDS             complement(10112..10585)
                     /locus_tag="PKK75_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HNZ83027.1"
                     /translation="MSNLSDIIEGFIKELMDANNNDAVEIQRNILAQQFDCSPSQINY
                     VLTTRFTNDRGYVVESRRGGGGYIRILKVRSSMDEELKRILNETIGDSITLNKALDLI
                     NALLEREVITEDEKRVMQSVLSDRVLNIVAYEDRNKLRADLLKKMILVSVENKDY"
     gene            10786..11070
                     /locus_tag="PKK75_05200"
     CDS             10786..11070
                     /locus_tag="PKK75_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HNZ83028.1"
                     /translation="MLGIHTHSFEGPTTVNDKHRHFYSGISSASPDTPGHDHNISAVT
                     TLNNGHTHSFSVTTAPAIPVKQGHFHPYTGTTTFNSGHVHYFRGNTTISG"
     assembly_gap    11093..11192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11265..>13855
                     /gene="clpB"
                     /locus_tag="PKK75_05205"
     CDS             11265..>13855
                     /gene="clpB"
                     /locus_tag="PKK75_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNZ83029.1"
                     /translation="MNAQKFTQKSLEAIQDAQNIAIQNQNMQIEQEHLLYALVDQEGG
                     LIGQLLFKMNIDTEQFKNKLKSIIDKMPRVSGPGREPGKVYVSQDVDRTLADAENQAE
                     RMKDEYVSVEHIMLSLLNNPKMSLKSLFNQFGFDKDKFLNSLSVVRGNTRVTSDTPES
                     TYDVLAKYGSDLVELAKNNKLDPVIGRDSEIRNVVRILSRKTKNNPVLIGEPGVGKTA
                     IAEGLALRIVKGDVPVNLQDRKIFSLDMSSLVAGAKFRGEFEERLKAVLEEVKKSEGR
                     IILFIDELHTIVGAGRAEGSMDAGNMLKPMLARGELHCIGATTLNEYRQYIEKDAALE
                     RRFQPVMVDEPTVEDSISILRGLKERYEVFHGVKIQDQALIAAAVLSNRYISDRFLPD
                     KAIDLIDEACAMIRTEMDSMPTELDEISRKIMQHEIEEAALKKEHDKLSEEHLADIQK
                     ELADMRAQFKEMKAKWENEKGAISKVQKLREEIEKVNADIELAERNYDLNKAAELKYG
                     KLPELQKSLQKEEESADNAKGSNNLLRDKVTDEEIARIVAKWTGIPVSKLMEGEREKL
                     LRLDEILHERVIGQNEAVDKVTEAILRSRAGIQNPDRPIGSFLFLGPTGVGKTELAKS
                     LAQALFDDEKNIVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVL
                     FDEIEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTIIILTSNIGSNYILEGINSGG
                     EITEEAKATVNSLLKRQFRPEFLNRLDEIVFYKPLKKSEISSILGLLFKDLNKRLGDK
                     QLSVSVSERAKDYIIDTGYDPVYGARPLKRFVQSSVETLIAKLIISEDLDPNTILQVD
                     YDGRQLI"
BASE COUNT         3716 a         2932 c         2451 g         4656 t
ORIGIN      
        1 gcaagtttgt ttttcgtttt ttcattaatg aaaaatagtt tcgcaattac ctactatcca
       61 ttaatatatg cattaaaatt tcaagtgtta taaaaaagga atgcaatata agattaggta
      121 tcgcggagaa tacaacatgg tttagtgcat cgcaggggaa tgcagcaaat ttgtttgctc
      181 cttgtgtcac acaaatttgg cattctgtgc ggttgaccta ccagccatat gttcttcgct
      241 atggctcgaa catatggggt taggtcatac aaaaaaacac accataaaat tatgatgtgc
      301 tataaaaaat attgttttgt aaacaagaaa aaataataaa aatattaagc gaacatttgc
      361 aggacacgac tataattctt agcgaatgaa ggtttaaaaa tccgttaggc agcttgcact
      421 gtttgagcgt agcgagtttg caagctgtag gatttttaac ggtcatgagc tattagaatt
      481 attcgtgtac gaaaccgtga gcgcatattt ttgttttttt tgcctgtagt aattatcaag
      541 ctaatgctta tggtaaatgt tataaactat tcattgcact agggagacat tagcctttgt
      601 taattatctc ttttacaatt tctgcggctt tttgcgggtc tgcctttccc tttgtttcct
      661 tcattatata tcctatgata gacccgaatg ccttctcctt gcctcccagg tatgattcaa
      721 cggcctcctt gtttccggaa acggctctca tgcaaatttc tttaagggca tcatcatcta
      781 tgccttccat atcttttttg gatatgaatt cattcacagg ctttcctgta tcaagcattt
      841 tttcaagagc attgcttgcc atgctggaat tgattttttt attttcaagc aagtaaatca
      901 attcatttat ataggcgggc ggtatggaaa tcctgcattc ttctttatct gagtctgtgg
      961 gaattcttct gaatacctgc cctaatatat aattggaaac caatttctta ttctttatat
     1021 ctttgcctgc ctcttcaaaa tattcggcaa tttttttgta ttttatcaga aggtcggcat
     1081 catccttggg gagaccatac tcattcataa atatatccat tttttcgtcc ggaagctgtg
     1141 gaagctggcc ctttaatctg tttatttctt cttctgtaac tttgatatta actaaatcag
     1201 ggtctctgaa atatctgtag tcatcagcat cttccttccc tctcatactt tcagtaatat
     1261 tcaggtcaga atcatacctt cttgtttcct gttcaattac acccccgctt tccaatatgt
     1321 ccacatgcct gttaaattca tattccattg cttttgctat gaaattcagt gagttcatgt
     1381 tctttatttc cgttctggtg ccatactttt cttgaccctt ttcccttacg gatatattaa
     1441 catcacacct cagtgaacct tcctgcatct taacgtcaga aacaccgata tatttcatta
     1501 tcagtcttaa cttttccata tactctaaaa cttcatcaat acttctgaaa tccggctctg
     1561 ttacaatctc aataagagga actcctcctc tgttgttatc tatgtaagtg tctcctcttt
     1621 catgaataag cttgcctgcg tcttcctcta tatgtattct gtttatatta atatttttcc
     1681 ccgatgaaag cttgatatat ccctcatagc agataggctt gtcaaattgt gatatttgat
     1741 atgctttagg caggtcagga tatacataat ttttacggtc catttttgaa acatttctta
     1801 ttttgcaatt agtggcaagg ccggccttaa ctgcgtattc cacaaccttt ttattaagct
     1861 ttggtaaagt cccgggcatt cccatacata tgggacaaca gtgagtgttt ggctctcctt
     1921 tgaattctgt cgtacactta caaaatattt ttgtattcgt agataattcg atatgtgttt
     1981 caagtcctgc aactaattca taactcatat ttccacctcc ggataaggag accttccgcc
     2041 tgtctttttt gtcagttttt caaattgata ggctgcattt agaaccttat aatcctcaaa
     2101 actgtttcct attaactgca tgcctatagg catcttattt tcgtcaaaac cgcacggaac
     2161 cgagactgca ggcagaccgg caatatttac cggaacggtg cagatatcca tcatatatat
     2221 ttctaccggg tcagctaatg cttctccctt tttataagca gtaaccggta cagaaggagt
     2281 taggatgaca tcacagcttt taaatatatt tttaaagtct tgaattactg aagatcttaa
     2341 gttttgtgcc ttcctgtaat atgcatcata acggcccgag cttagcgcat aggtccctaa
     2401 aagtattctt ctttgaactt ccctgccgaa gccttcgctg cgattcttgc atatcatttc
     2461 atttatatct ttgtagctgt ctgttttata tccgtatctt ataccgtcat acctgcctaa
     2521 attagatgat gcctctgcac atgaaataat aaaataagca gggagaacag catggagcct
     2581 gggcatttcg aagtttatta tttcagcccc tgcatcttgg tatgccttta ttgcttcttc
     2641 taatgagctt tttacttcac catttattcc ttcaaaatat tcctttgcta taccgatttt
     2701 aagcccctta acacttttgt ccagtttatc acttgcagtg gttttgcttc ctgttgatgt
     2761 ggaatccttg gggtcataaa ttgatatagt atcgtagact attgcagcgt cctctgcaga
     2821 ttcagttata ggtccaacct ggtctaggct tgaagcatat gcaacaactc catatcgtga
     2881 tacagtaccg taagtaggct ttaatccaac caggccgcaa aagcttgcag gctgcctaat
     2941 ggagcctccg gtgtccgaac ctatgccaaa tgccgccata ttccctttaa ctgaagcagc
     3001 tgcccctcct gagcttcctc ctgcaacata atccgtattg ttaggattca aggtgcctcc
     3061 aaaaattgaa gtttcacagg atgagcccat ggcaaactca tccatgttgg tctttcctaa
     3121 aagcacagca ttttggtttt ttaaaatttc ccaaaccgtt gcatcatata ttggataata
     3181 acctttaagc atccttgagc agcatgttgt ttcaatatcc ttagttgata tattgtcctt
     3241 tagagacatg ggtatccctt ccaggggcat cagcttttcc ccagctctta ttctttcgtc
     3301 aaccttttct gcagttttca gtgcctcatc agccgttaca tttacatatg cattaataat
     3361 tttattttgc ttttcgattg ctgttaaata cttctttgtc agttctacac atgaaatatc
     3421 tttgttttcc aataactttt gtatatgttt tattttgctc aaattttcac cccctgaggc
     3481 tcttcctgac tacaaaatat ccgttttctc cgccttctct gtttttcaaa atttcatccc
     3541 tgtcataaga atccacaaca atgtcttcgt taaatgcatt ataaatattg ttgatatctc
     3601 cgaattcgtt atcctggtct tcaacaacac tgtttattaa gtctgcgtac tctatcactg
     3661 ttgacatatc ttttataagc aggtcgattt catcatcctt cactgacaat ttggcaattt
     3721 tagctatacg caaaacttct tccttcgtta tcattttctc acctacctca attttatatg
     3781 aacttctctc aattgctgtt cagtaactgt gcatggtgca tttgtcatta aatcctgggc
     3841 attgttattc attgggaagg ctataacctc tcttatgttt tcttctcccg taagaagcat
     3901 tataattctg tcaatccccg gagccatgcc tgcgtggggt ggtgctccgt atttaaacgc
     3961 attgaataag gaggcgaatt ttgtctgcag ctcatctttg gtgtatcctg ctatttcaaa
     4021 tgctttaatc attatatcca aatcatggtt tcttacagct cccgatgaaa gctctattcc
     4081 gttacataca atatcatact ggaaagctat aatgtcaaga gggtcttttt caatcaatga
     4141 ttttagtcct ccctggggca ttgaaaatgg gttgtgggta aatgccgtgg cccctgtttc
     4201 ttcgtctact tcatacattg gaaaatcaac gataaaacac atttcatacc tgctttcatc
     4261 tattaaatca agcctctttg ccagctcggt tctaatctgc cctgctagct tgggtgcatg
     4321 gtcctttgta tctgctatga aaaacaatac gtctcccgga tttaagtttg ctttatgagc
     4381 cagttctttt ctgtcttcct gagaaagaaa cttatctata ggaccctggt atttcatttc
     4441 atcgtcaacc ttgatgtagc caagcccctt catgccgatt tttgtagcaa atcccagcat
     4501 atcttcgaaa aaacgctttg gctgtgatga acagtcaggc acattgattg ctctcaccgt
     4561 tttcttcctg aatggtgcaa aatctgtttt ctcaaacata gggcttatat ctataatttt
     4621 cagcgggttt ctcaaatccg gtttgtcaga cccgtaaaca agcattgctt cttcataagg
     4681 aattctttta aaaggaggtt tggatacttc cctgttagaa aactttgtaa atgtttggta
     4741 caatacttct tccgcagccg aaaatacatc ctcctgagtt gcaaaggaca tttcaaaatc
     4801 aagctgataa aattctcccg gtgaccggtc agctcttgca tcttcgtctc tgaagcaggg
     4861 agctatctga aaatacttgt caaatcccga aaccatcaag agctgcttaa atacctgagg
     4921 ggcttgaggc agagcgtaga actttcccgg atgcttcctg cttggtataa tataatctct
     4981 tgcaccttca ggagaagatg atgttagaat cggtgtttgg atttccaaaa aacccatatc
     5041 tgtcatcttg ctccttataa aggatataac ctgggaccgg agaataatat ttttttggac
     5101 ctctacgttt cttaagtcca aaaacctgta tttcagccta agatcctctt ttgtttcttt
     5161 cgatgttgta acatcaaaag gaaggaattc tctgaccttg ccaagcacat ctatgctgtc
     5221 tgcttcgatc tcaatgtatc cagtatcaag tttcttgttt attgtgtctt catctctttc
     5281 agcaacaaga ccggttaccc ggattgcact ttctcttggt attccctcaa gcatttcttc
     5341 atcttccaca actacctgta caatgccgta ctggtctctt aagtccacaa acttaacgcc
     5401 tccgtggtct cttacatttt caagccatcc tgataatctg acctgtttac ctatatcact
     5461 ttcccttaac tgcccgcagg tgtgagtgcg gtactcattt gctctcagca taataatttc
     5521 ctctttctac ataataatat tttttaattt acaaatttta tcgccttggg taatacgcaa
     5581 tctactttca ccattttatc tatttgggga cattcctctg actgcagaga ctatccctcc
     5641 ggtagaggtg tgtccccata cctattttgt cgaagaaatt gggttagggc atacgcagtc
     5701 tcaatcacca aattatttgc tccttccgtc gcataatttg ggatttcgtt gcggttgacc
     5761 taccatcaag actttctccc tccggtcgaa gtcttgggtt agggcataca aaaactcctc
     5821 catccccgga aagggacgaa ggagttgaat ttcgcggtac caccctaatt agcatatact
     5881 tacatatagg ctcactcaaa agacactgac atgtctttgc cgctttatcg ctcggatgcg
     5941 tttgagccta ctaatgcatg tgcagttttg gtcaaaagct cagggatgtt cttcctttat
     6001 aatcattgta atagactttc accggcgcta tctctctgtg actttaatta taaagtactc
     6061 ttcccatcat cgctaaatat ttccttgttt ttataaatct aatatagttt ttataatttg
     6121 tcaagaggat ttttttattt tttatcgttt gtaaaaatta aatccttgcc atcggctgta
     6181 atctttattg tgtcagattt ctgtattgct ccttttaaaa gctcttctga tagtttgtct
     6241 tcgatttgtc ttcttatttc tctttggagg ggcctcgctc cgaatacctg gtcaaatcct
     6301 gcatctgcca gcaggtcctt tgccttgtca tcgacctcta tatggatgcc catctcatca
     6361 aggcgctttg acagattgtt tatcatcagc gacacaattt cttttatatg atccttattc
     6421 aatgaatgga agacgattat ttcatcgata cggtttaaaa actcaggtct gaatgttctt
     6481 tttaattcat ccagcacatt ttccttcatt ttctcgtatt cgtctttttc tgcatttgtg
     6541 gaacttgctg caaaacccat agttttttgc ttcttaatgg tagatgcacc cacattggat
     6601 gtcataataa ctactgtgtt cttaaagttc actgttcttc cctttgcatc tgtcagccgc
     6661 ccgtcatcca gtatttgcag caggatgttg aatacatccg ggtgggcctt ctcgatttca
     6721 tcaaacaata ttacagaata tggctttctt cttattcttt ccgttaactg acctccgtca
     6781 tcgaatccta catatcccgg aggcgatcct atcatttttg acacggaatg cttttccata
     6841 tattcagaca tgtcaaccct tatcattgca tcctcgtcac cgaacatggc ttcggcaagc
     6901 gcctttgaca gttcggtttt tcccacgcct gtaggaccta aaaatataaa tgagcctatt
     6961 ggttttttcg ggtccttcag acctaccctc gaccgtctga tagcctttga caaggcttca
     7021 acagccggct cctggcctat tacccttttg tgaagaattt cctccatatt aagaagtctt
     7081 tccgattcat ctccctggag ctttttaacc ggaattcctg tccattggga tactacgtct
     7141 gctatatctt cgtaggcgat ttttatatcc tttatgtttt ttgcagccca taatttcttt
     7201 tgtttatcaa gttcatcgga tagtttcttt tcttcatccc ttaatgaggc tgccttttca
     7261 aaatcctggt tgtttatagc agttcttttt tcagtgtcaa cttcttttat tttatcttcc
     7321 atttccttta gttctttggg ggcagtggat gatttcaatc ttaccctgga agccgcctca
     7381 tctattagat ctatagcctt gtcaggcaga aatctgtctg aaatatatct gtgggaaaga
     7441 tttgctgcag catctattgc ttcgtctgtg attgccacct tgtgatgagc ttcatattta
     7501 tctcttaatc ccttaagaat cctgatagtg tcttcaactg ttggctcctc aactattatc
     7561 ggctggaatc ttctttcaag agccgcatcc ttttcaatgt gttttttata ttcgtctatg
     7621 gtagttgcac ctacaacctg aagctcccct cttgccatca cgggttttaa aatgtttgat
     7681 gcatctattg ctccttctgc agctcccgca ccgattattg tatgcatctc atcgatgaac
     7741 aaaataatat tgcctgctgc ccttatttcg tcaatggcat ttttaagtct ttcttcaaat
     7801 tctcccctgt attttgctcc tgctaccatt ccggccatat ccaaagtaat tacccgctta
     7861 tccttcaaag tttcaggaac gcttccttta ataatctcct gggccaaacc ttccgcaatg
     7921 gctgtcttgc ccacgccggg ctcacctatg agacaagggt tgttttttgt tcttcttgaa
     7981 agtatttgaa ttactctttc aattacattt tcccgcccga taaccgggtc taatcttcct
     8041 tccttggcac gctgatttaa atcagtgccg tatttatcta tggtcctggt atctgagttt
     8101 tcctgtgcca taccgccgcc catattggga ttaaccggct cctggccccc tcccccatgc
     8161 atggacataa gaacctcatt ttttaaatct tttaaattca ctgctgattt aataatctga
     8221 gctcctatac cttcaccctc ctctataagc cccagtaaaa tatgctctga gcctacataa
     8281 ggcgttttaa gctgtgctgc aaattggcgg gcaagctcta ttatatattt gcttcttggg
     8341 cttatggtaa tgtattcagg tatagaatct cccatgcctg ttgaattttt tattatttct
     8401 ctgacttttt caacatctaa tttatctctg agtaattttc ctgcaatacc ttcctcttca
     8461 atcatcagac caagtaaaat atgttcagta cctaaaacat tttgtctgaa tcttttcact
     8521 tcttcctgag caagggctaa tgcatttttt gcagaattat ttaatcctcc catcatatct
     8581 ttcatctcct ttttgtatat attaactcaa taattcactt ctaattattt ccgctctttt
     8641 tttatctctt tccttggggg aaagttcttt ttcttcaata gttgaaatat tatttggctg
     8701 gctccaaacc atgagccggt atatattctt gccctttacc atgggtgtta ttcccatttc
     8761 gcttcccagt ttaatcagag acaagagttt cattgcttca tccgaagata tttttcttgc
     8821 atttgtcaat aaaccgtaag ctctgtaaaa atcatcctcc aaatcatcat aattattctt
     8881 caaaaggtgg tctcttgttt tattttcctt ttcaataatc tgcattacaa tttgatttat
     8941 cttttcaatc aatgtttctt cagaaacacc cagtgttccc tggtttgaaa tctgatacag
     9001 atgtcctatg gactttgttc cctcaccata tactcccctg actgcaaccc ccagttgtga
     9061 gaccccgtag agaagcttgt cgatttggtt tgttatggac agtgcaggca ggtggagcat
     9121 taccgatgcc ctcatgcctg ttcctatatt agttgggcag gctgtaatat atcccaattc
     9181 attgtcaaaa gcataatcaa cattttcttc gattatatca tccacattat tgctgatttc
     9241 ccagcattcc ctcaaggata gaccatcttc tatggtttga attctaatgt ggtcttcctc
     9301 gtttatcatt actgaaattt tttggtcggg acttagaaga aatgcgcccg catccttttt
     9361 atttgccaat gcaggagata taatgtggtt ttctatcagg gaatttttct tgatatcaga
     9421 aagatcctta atgtaagtca gcttgtaatt tttatcatgc ttgcttaagg cattgcttat
     9481 ctctccaatt acgcagttag cttctgcttc actcatgatt atggggaatt tgtagttttt
     9541 taaattccgt gcaagtctga ttctgctggt tattacaata tcattgtttt ccaaatcatt
     9601 catccccctt tttattttcc atctcgtgaa ttttgtctct aagttctgcc gccagttcat
     9661 attcctccct atcaacagca gccttcaaat catttttcag cctttcaata tccatttgca
     9721 ccttgtagtc tccccctgct cttttgggga ttttccctgt atgtctgtca aatccctgca
     9781 tactttttat aacaggcttc aatcttccct tgaatgagtc aatgcaccgg ctgcatccga
     9841 atttgcctgt atttctgaag ttttcgtatg tggtgccgca atttgggcag gactttgccg
     9901 gcaggtatgt tttattgtta aaggtattgt tcagcatagc agagaacaag ttttccattg
     9961 aaatatttga gtttaaaatt gcctggttct ttttcgcaca ttcctcgcac agatggcttt
    10021 ctgtcttttt tccgttaatt atgtgagtta aatgcacatt ggcttcattt ttgccgcatt
    10081 cgttacataa catagtaaac cctcctgtca tttaataatc tttgttttct acggatacca
    10141 atatcatttt tttcaataaa tcagcccgca gcttgtttct gtcctcataa gcaacaatat
    10201 ttaaaaccct gtcgcttaaa acactttgca taactctttt ttcgtcttct gttattactt
    10261 ctctttccaa aagtgcattt attaaatcaa gagctttatt tagtgtaatg ctgtccccta
    10321 ttgtttcatt caatatacgc ttcaattctt catccattga ggacctgacc tttaaaattc
    10381 ttatatagcc gcccccgcct ctcctgcttt ccacaacata tcccctgtca ttggtaaacc
    10441 gggttgtgag aacgtagtta atttgagatg gagaacagtc aaactgctgt gccaggatat
    10501 ttctttgaat ttcaactgca tcattattgt ttgcatccat taattctttt atgaaacctt
    10561 caattatatc gcttaaattt gacacgccgc acctccttac tcaagtcgat atgacttttg
    10621 ttctgctgaa tttgactttg actatctttg accttaagca tattctaaca cctttacaaa
    10681 ataaatgcaa gggttttttt gaaaaacact tcttacacca gtggcaaaga ataatacagg
    10741 acataaaata ataaaacaag aaattataaa agaggtgatt ataaaatgtt aggtattcat
    10801 acccacagct ttgaaggccc taccactgtg aatgacaaac acaggcattt ttacagcgga
    10861 atatcttctg catcccctga tactccggga catgatcata atatatctgc cgtaacaaca
    10921 ttaaataacg ggcacaccca cagcttcagc gtaaccacag ctccggcaat tcccgtaaaa
    10981 caagggcatt tccatccgta taccggaaca acaactttta acagtggtca tgttcattac
    11041 ttcagaggca atactacaat ttccggataa aaaaataata atcggcagca ttnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtttaaat taataacttg atggtatcat
    11221 aatattatca gataaaatta acatttaaag tatggaggat tataatgaat gcacaaaaat
    11281 ttacccaaaa atcattggaa gcaattcagg atgcacaaaa tatagcaata caaaaccaaa
    11341 atatgcaaat tgagcaggag catttactgt atgcattggt tgaccaggag ggcggtttaa
    11401 taggacaact tctttttaaa atgaatattg acacggagca gtttaaaaac aaactgaaaa
    11461 gtataataga caaaatgccc cgtgtatcag gacccggcag agaacctgga aaggtctatg
    11521 tatcacagga tgtggacagg acattggcag atgctgaaaa tcaagcagag agaatgaagg
    11581 atgaatatgt ttctgttgag catattatgc tttccttgtt aaacaatcca aaaatgagtt
    11641 tgaaaagttt atttaatcaa ttcggatttg acaaggataa attcttaaac tcactaagtg
    11701 ttgtaagggg caatacaaga gttaccagtg atactccgga atcaacttat gatgtattgg
    11761 ccaaatacgg gtcagatctg gttgaattag ccaaaaataa taaattggat ccggtaatcg
    11821 gccgtgactc ggaaattaga aatgtggttc gtattttatc gcgaaaaacc aagaacaatc
    11881 cggtgttgat aggcgagccc ggtgtcggca aaacagctat agccgaaggt ctggctctcc
    11941 gtatagtcaa aggtgatgtg ccggttaatt tgcaggatag aaaaattttc tcgctggata
    12001 tgagttccct ggtagcagga gcaaaattca ggggggaatt tgaagaaagg cttaaggcag
    12061 ttttagaaga agtgaaaaag agtgaaggca gaataattct ttttattgat gagcttcata
    12121 caattgtggg agcaggcagg gcagaaggct ccatggatgc aggcaatatg ctcaagccta
    12181 tgcttgcaag aggagagctt cactgtatcg gtgcaacaac attaaatgaa tacaggcaat
    12241 acatagaaaa ggatgctgct cttgaaagga gatttcagcc ggttatggtg gatgagccca
    12301 cggttgagga ttcaatatca atactcaggg gcctcaaaga aagatatgaa gttttccacg
    12361 gcgtgaaaat tcaggaccag gcattaatag ctgcggctgt attgtccaac aggtatatat
    12421 cagaccgttt tctcccggat aaggccatag atttaattga tgaagcatgt gccatgatac
    12481 gtacagaaat ggattcaatg cctacggagc ttgatgaaat atcaagaaaa ataatgcagc
    12541 atgaaattga agaagcagcc ctgaaaaaag agcatgataa actttcagaa gaacacctgg
    12601 ctgacattca aaaggaattg gctgacatga gagctcaatt taaggaaatg aaggcgaaat
    12661 gggaaaatga aaagggagca atttcaaagg ttcaaaagct tcgtgaagaa atagaaaaag
    12721 taaatgctga tatagaactg gcggaaagaa attatgattt gaacaaggca gccgaattaa
    12781 aatacggaaa acttcctgag ctgcaaaaat cactgcagaa ggaggaagaa tcagcagaca
    12841 atgcaaaggg ctcgaataat cttttgagag acaaggtgac tgatgaggaa attgcacgca
    12901 ttgtggcaaa gtggacaggt attcctgttt ctaaattaat ggagggagaa cgggaaaaac
    12961 ttctgcgcct tgacgaaatc cttcacgaaa gagtaatcgg gcagaatgaa gctgtcgaca
    13021 aggtcactga agcaatacta agatcaagag caggtattca gaaccctgac agacctatag
    13081 gttcattctt gttcctgggt cctacagggg taggaaaaac agagcttgcc aaatcattgg
    13141 cccaggcctt gtttgatgat gaaaaaaata ttgtgagaat cgatatgagt gaatatatgg
    13201 agaagttttc tgtttcaaga cttatcggag cgcctcccgg atatgtgggt tatgaggaag
    13261 gggggcagct tactgaagct gtaagaagaa agccctattc agtcgttttg tttgatgaaa
    13321 ttgaaaaagc ccatccggat gtattcaata ttttgcttca ggttcttgat gacggcagaa
    13381 tcaccgattc ccagggcagg acagtggatt ttaaaaatac gatcattata cttacatcaa
    13441 atatcggttc aaattatata cttgaaggta taaacagcgg gggtgaaatt acggaggaag
    13501 caaaggcaac tgtaaacagt ctgctgaagc gtcagttcag gcctgaattc ttaaaccgcc
    13561 ttgatgagat tgtattttac aagcctttga aaaaatcaga aatttcatca atacttggtt
    13621 tgttgttcaa ggaccttaac aagaggctcg gcgacaaaca actgtctgtt tccgtatccg
    13681 aaagggctaa agactatatc attgatacag gatatgaccc tgtttacggt gcaagaccat
    13741 tgaaacgttt tgttcaaagc agcgtggaaa cactgattgc aaaattgata atatcagagg
    13801 atttagatcc aaacacaatt ctccaagtgg attacgacgg caggcagcta attat
//
LOCUS       DAOPUT010000043        13600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_4615_length_13600_cov_18.668586, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000043 DAOPUT010000000
VERSION     DAOPUT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13600
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4615_length_13600_cov_18.668586"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..521
                     /locus_tag="PKK75_05210"
     CDS             90..521
                     /locus_tag="PKK75_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNZ83030.1"
                     /translation="MILSTKGRYGLKAAFELSKNYGLGPVSLKKISDKYGISESYLEQ
                     LFAKLKKSGYIDTVRGPQGGYLLAKEPGEITVGMILRTLEGEITTSECVSKEVCSRES
                     ICATRVIFEKIEKSINDVIDNITLADMFEEQKNIITEGKNE"
     gene            514..1701
                     /gene="nifS"
                     /locus_tag="PKK75_05215"
     CDS             514..1701
                     /gene="nifS"
                     /locus_tag="PKK75_05215"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNZ83031.1"
                     /translation="MSKKLYLDNAATTRVRKEVIEEMSRYFDELYGNPSSQLYELGRK
                     SKEAIEKARKTLADFLNADEKEIYFTAGGTESDNWALKGVAFANLNKGKNHIITTKIE
                     HHAILHTCEYLEKFGIETTYLDVDRYGLIDIEHLKESIRPETMLISVMFANNEIGTIQ
                     PIEEIGEIAKEHGILFHVDAVQALGSVKIDVNKQHIDLLSMSAHKIRGPKGIGGMFIR
                     KGTRIDNFIHGGGQERGRRAGTEGVQNIVGFGKAVEIAARDFDHHVERLTVLRNRLIE
                     GIKNNIPDVVLNGHPTRRLPNNVNFSYKYVEGESILLLLDMEGISASSGSACTSGSLD
                     PSHVLLATGLDHGTAHGSIRFTLSEEITEEDIDFTVETMKKIIIKLRAMSPIKNDEDL
                     HKN"
     gene            1720..>2142
                     /gene="nifU"
                     /locus_tag="PKK75_05220"
     CDS             1720..>2142
                     /gene="nifU"
                     /locus_tag="PKK75_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HNZ83032.1"
                     /translation="MYSEIVMDHFRNPRNAGTMENPDGVGQVGNPKCGDIMKIYLKIN
                     KNEIIEDVKFETFGCGSAIASSSIATEMIKGKSVHEAVELSNKAVIEALGGLPPVKIH
                     CSVLAEQAVKSALYNYAQKNNKTIKGLENYKPEDDEIHC"
     assembly_gap    2143..2242
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2323..4962
                     /gene="alaS"
                     /locus_tag="PKK75_05225"
     CDS             2323..4962
                     /gene="alaS"
                     /locus_tag="PKK75_05225"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNZ83033.1"
                     /translation="MDKLDRFSINNIRKEYLNFFKSKGHLTEQSFSLIPKNDKSLLLI
                     GAGMAPLKKYFTGAEVPPSKRMATCQKCVRTGDVDNVGLTARHLTFFEMLGNFSFGDY
                     FKEEAIAWAWELLTDVLKIEKDRLWVTVYLEDDEAENIWHTKVGIPMERIVRLGKDDN
                     FWELEVGPSGPCSEIYYDTGDDRGCGSPDCKPGCDCNRYIEIWNLVFTQFDKDEKGVY
                     HPLPNPNIDTGMGLERVAAVLQDKPTVFDVEPLNLIVNKASEICRIPYGQNKKSDIAM
                     KIICDHSRAATFMIGDGVIPSNEGRGYVLRRLIRRAIRRGKSLGINGEFLTGTAKVVI
                     DNWKEAYPVLSEKESYILKVLSLEEERFKKTIDQGLELLMKEIQILKETDKKELEGKV
                     AFKLYDTYGFPSELTEEILKENGFSLNKEQFIKVMEDHRQMARDARKSTGASGWKEND
                     FTIDTDKTVFTGYNNLEDSSEVVAIVVGSEQVNTINEGQSGIIVVDKTPFYAESGGQT
                     GDRGEFIADDMLAKVTDTHKLNNDIFLHETNVVKGSISVGDSITAKVDADRRKNTAKN
                     HTCTHLLHRVLRQMLGDHVNQAGSYVSEDRLRFDYTHFEAVDHKTLKEIEKRVNEAIL
                     ADYMVRTDILNIEEAKKSGATALFDEKYQEKVRVVSVGDFSKELCGGTHIDETAKIGL
                     FKIVSEGSIASGIRRIEAITGRAAINYLRELDNLTDELSISMKTTKDELLNKVNMLKK
                     EAKDKDKEIQKLNNELLRGNISEILDKYEEINGIKLFALYLKDKDANTLREIADRIKD
                     KNESCAVILASDLGDKVLFVSAVTKDLVEKGVHAGNMVKEAAVIAGGGGGGRPDFAQA
                     GGKNPGKIDEAIEAVKKQLSMNK"
     gene            5243..5506
                     /locus_tag="PKK75_05230"
     CDS             5243..5506
                     /locus_tag="PKK75_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HNZ83034.1"
                     /translation="MESNTNNTAKFDYSMGSLNEAREILSQVYKSLKEKGYNPNNQLI
                     GYILSGDPTYITNHNNARSLIRKIERDELLEEILGVYLDAIDL"
     assembly_gap    5579..5678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5776..6723
                     /locus_tag="PKK75_05235"
     CDS             5776..6723
                     /locus_tag="PKK75_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ83035.1"
                     /translation="MKYINLFEKRNVSKLCYGTLSLSNLQNSDTLENRVRLLNYSLEK
                     GINFFDTAELYNNYDILKEFIKGKDRNKLVIASKSYAYDKNTAEYSINKALKEMDTDY
                     LDVFMLHEQDNGNNFIGHYKAVDRFMKYKEDGIIKHFGISTHRVEAVRDSIKFKEIEF
                     IFPLFNYKGIGIQDGNIEDMSEALKKAKEYNKFIMAMKIYGGGHLINEAEKAFKYVDN
                     LNYIDSIAAGMGTVEEIDANISYMDNNELNENIKNKIRNQKRTLVVEEWCAGCGKCAK
                     KCRQNALEIVDGKCRVDMEKCVLCSYCAGECKDFYIKIV"
     gene            6731..7144
                     /gene="ruvX"
                     /locus_tag="PKK75_05240"
     CDS             6731..7144
                     /gene="ruvX"
                     /locus_tag="PKK75_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNZ83036.1"
                     /translation="MERLMGLDVGDKTIGVAVSDLLMITAQSVTTIKRTNIKNDINEL
                     KKLINEYQVTKIVLGIPKMLDGTIGIQGEKVLNFLEKMKRYIDLPVELEDERFTTTIS
                     ERMLIEADVRRKKRKEVIDKLAAAQILSSYMQRTK"
     gene            7160..7402
                     /locus_tag="PKK75_05245"
     CDS             7160..7402
                     /locus_tag="PKK75_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HNZ83037.1"
                     /translation="MDNIIELINDEGNLEKLVVEVTFHIDDILYAVLHKINSDEGMIY
                     YVEEMEDGSIVLIKVEDEDEIKEVQEIYEGIADDLI"
     gene            7523..9199
                     /locus_tag="PKK75_05250"
     CDS             7523..9199
                     /locus_tag="PKK75_05250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNZ83038.1"
                     /translation="MAEKKQKLKIIPLGGLGEIGKNSTVIEYNNNIIVIDCGMAFPGD
                     EMLGIDIVIPDITYLIKNKEKIKGIILTHGHEDHIGSIPYTLKKLNVPIYGTKLTLGL
                     VENKLLEHKIEDAFLNPIKAGDTFDLGCFKITAVRVNHSIPDAVAYFIDTPIGTVVHT
                     GDFKIDYTPINEEVIDLGKFADIGRKGVLLAMSDSTNVERLGFTESEKTIGQKFMEIF
                     KHCDSRIIVASFASNIHRLQQVIDAAVFNKRKVAISGRSMVNAIEVSLDLGYLHAPEG
                     TIINVNEVKSLKDNEIVILTTGSQGEPMSALTRMANNDHKKIQIKPGDLVVISANPIP
                     GNEITVSRVINMLYEKGARVLYDALTQVHVSGHARRDELKLMLTLLKPKFFIPVHGEY
                     RHLVQHANLANELGMPIENTIIGNNGDVIELTSKSIVKNNTVTSGNILVDGLGVGDVG
                     NIVLRDRRLLSENGLIVVVLSTERGTGRILAGPEIVSRGFVYVRENIDLIEESKTVIN
                     KALEKCQDTNVKEWNNIKMVIKDSLSSFIYDKIKRSPMILPIIVEVNPDE"
     gene            complement(9327..10025)
                     /locus_tag="PKK75_05255"
     CDS             complement(9327..10025)
                     /locus_tag="PKK75_05255"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNZ83039.1"
                     /translation="MISNDNIETERTRICLTIGRIAPDFTAMTTEGPITLSQYRGKWV
                     LFFSHPGDFTPVCTSEFIAFAQLQPEFEKRNVQLLGISIDSNPAHIGWLYDIFQMNGI
                     VMPFPLVADRNAQISALYGMVNPDRIYEQSVRDVFIIDPNQRIRAILTYPGTNGRNMY
                     ELLRLIDALQVSATYGVSTPANWMPGDPVIVPVANTLDEAIARGTDSESLDLNCLAWW
                     YCFTDASELTPVKS"
     gene            complement(10181..10423)
                     /locus_tag="PKK75_05260"
     CDS             complement(10181..10423)
                     /locus_tag="PKK75_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83040.1"
                     /translation="MDRFNKSSTPDLTRVVNSDVIKLRRDGQYKLTSDINGKLTPNLN
                     VISWKSNQRMEDKSIVNNKPSQFGPILGVNFHNIKR"
     gene            10570..11586
                     /gene="mltG"
                     /locus_tag="PKK75_05265"
     CDS             10570..11586
                     /gene="mltG"
                     /locus_tag="PKK75_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNZ83041.1"
                     /translation="MKKIIALLCVLVIIAAAYLYLNNYIVESLTAVDINDKTAITVEI
                     PSGSTTNDIAEILFENNLISNIRTFKYYAERTGSDSKLKAGSYVLSRNMNADELLDSL
                     IKGGSSGNTYNITIIEGLTNEDMAQVLSEQTGLSYDKFIELMEAPETFREDYEFLNDT
                     SVSNLQGYLMPDTYNIYIDSSEEDIIRVLLKQFDEFYLDEIKPRMGNTGLSFEEVINL
                     ASIVEKEALLDEERDEVAKVFLNRLDINMKLQSCATVNYANGEWKERLTYEDIEIDSP
                     YNTYIVEGLPPAPINSPGRVSIISVLEPADVDYLFFVAKGDGSHYFSNTYDEHIDAAE
                     EYLN"
     gene            11589..12236
                     /locus_tag="PKK75_05270"
     CDS             11589..12236
                     /locus_tag="PKK75_05270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNZ83042.1"
                     /translation="MDNINREYIDKYINSLIPVDNEKLNEFREYCEERNLPIIHKEVG
                     QYIKLVINQLNAKSIVEIGTNVGYSAIFMSKVMKGEGRVVTFERSEKFYKEAIKNIAD
                     FGLENNIDVHLGDAVDILNETEGTFDMAFIDAAKSYYRVFFDKCLKMMKPGGIILSDN
                     VLYQGMIASDELVVRRKKTLVRNLRNYLEYISHDNRFVTSILPLGDGLAVTYIKD"
     gene            12246..13463
                     /locus_tag="PKK75_05275"
     CDS             12246..13463
                     /locus_tag="PKK75_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNZ83043.1"
                     /translation="MLPKLLAPAGNLDKLIMAVQYGADEAYFAGKSMGMRAGGKNFTK
                     KDLELGVEYCHKYGRKANITVNIVPHNDDLKGIEGYLKYLHKIGTDALIVADPGVVEI
                     VKETIPDMRIHLSTQANTTNYHTANFWYKQGVNRIVLARELTFDEIETIINNTPDDLE
                     FETFVHGAMCISYSGRCLLSSFMTGRFSNKGDCAQSCRWKYNLVEEKRPGEYYPIEET
                     DEGTFIMNSKDMCMIEHLQKFKDLGIKALKIEGRNKSEYYTAVTVRSYRNALDELEYP
                     ESERNTSYWKEEVEKTSHRDFSYGFFFGNPYKDGQSYTNSSYIRTYDVVGIIRSYDQT
                     SKTATVEQRNKFSEGDLLECLGPKGKHFELTARNLTDEDNNKIDSAPHPQQTVKLYME
                     ESVEPYYILRKKV"
BASE COUNT         4798 a         2092 c         2905 g         3605 t
ORIGIN      
        1 tttttttaat gttgacaaaa ttactggggt atcttataat caattgtacc ctaccgaaac
       61 agtagggatt gactgaaagg agaagagaaa tgattttatc cacaaaggga agatatgggt
      121 tgaaagcagc atttgaactt tcaaaaaatt atggattagg accggtttct cttaaaaaaa
      181 tatcagataa gtatggtata tcagaaagct atttggagca gctgtttgca aaactaaaaa
      241 aaagcggcta tattgacacg gtcagaggac ctcagggcgg atatctcctg gcaaaagaac
      301 ccggggaaat aacagtgggc atgatactta gaacacttga gggagaaata acaacttctg
      361 aatgtgtaag caaagaggtt tgttccaggg aatcaatttg tgctacaagg gttatatttg
      421 aaaaaataga aaaaagtata aatgatgtca ttgataacat aactttagct gatatgtttg
      481 aggagcaaaa aaatattata acggagggaa aaaatgagta agaagcttta tcttgataat
      541 gctgcaacaa caagggtaag aaaagaagta attgaagaaa tgagcaggta ttttgatgaa
      601 ttgtacggca atccatcaag tcagttatat gaacttggca gaaaatcaaa agaagcaatc
      661 gaaaaggcaa ggaaaacttt agcagacttt ttaaatgcag atgaaaaaga gatatatttt
      721 accgcaggag gtaccgagtc tgacaactgg gctttaaagg gagttgcctt tgcaaacttg
      781 aacaaaggca agaaccatat aattacaact aaaattgagc atcatgccat acttcatacc
      841 tgtgagtacc tggaaaagtt cggaatagaa acaacttatc ttgatgttga cagatacggg
      901 cttatagaca tagaacatct gaaagaatcc ataagacctg aaacaatgct tatttctgta
      961 atgtttgcaa acaatgaaat agggacaata caaccaattg aagaaatagg ggaaattgct
     1021 aaagaacatg ggatactgtt tcatgtagac gctgttcagg ctttgggaag tgtcaaaata
     1081 gacgttaata agcagcatat agacttattg tccatgtcag cacataaaat tcggggccca
     1141 aagggaatag gcggaatgtt tattagaaag ggaaccagga ttgataattt tattcacgga
     1201 ggcggtcagg aaagaggcag aagagcaggt acagaaggcg tacaaaatat cgtgggcttc
     1261 ggcaaggctg ttgaaatagc tgccagggat tttgaccatc atgtggaaag actcacggtt
     1321 ttaagaaaca gactcataga aggaattaaa aacaacattc ccgatgtggt tcttaacggc
     1381 catccaacca ggaggcttcc aaataacgtg aatttctcat ataaatacgt ggaaggagaa
     1441 tccatattat tgcttcttga tatggaagga atatcggctt ctagcggctc agcctgcact
     1501 tcagggtctt tggacccgtc acatgtgctg ctggcaacgg gtcttgacca cggaacagcc
     1561 cacggctcaa ttcgttttac actaagcgag gaaataacag aggaagacat agattttacg
     1621 gttgaaacaa tgaaaaaaat aattattaaa ctaagggcaa tgtcaccgat taaaaacgat
     1681 gaagacttgc acaaaaacta aggtagcgga ggataaaaaa tgtattcaga aatagtaatg
     1741 gaccacttca gaaacccgag aaacgcaggc actatggaaa atccagatgg cgtaggccag
     1801 gtgggaaacc cgaaatgcgg ggatataatg aaaatatatt taaaaatcaa taaaaatgaa
     1861 ataattgagg atgtaaagtt tgaaacattc ggctgcggct cggcaattgc ttcatcaagc
     1921 atcgccactg agatgataaa ggggaagagc gtccatgaag cggttgaatt atcaaacaag
     1981 gcagttatag aagctttggg agggctgccg ccggttaaga ttcactgttc cgttttggct
     2041 gagcaggctg tcaaatcagc actttataat tacgcacaga aaaacaataa aacaattaaa
     2101 ggtttggaaa attacaagcc ggaagatgac gaaatccact gtnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnttgacaat atgggaaata atacattata atttcataat
     2281 aaggtacata ttgcttatac tatgaaacgg agggaattta tagtggacaa attagataga
     2341 ttttcaataa ataatattcg aaaagagtac ttaaattttt ttaagagtaa aggccattta
     2401 acagagcaaa gcttttctct aattcctaaa aatgacaaaa gtttgttgtt aataggtgca
     2461 ggcatggctc cgctgaagaa atactttacg ggagctgaag taccgccttc aaagagaatg
     2521 gcaacctgcc agaagtgcgt gagaaccggt gatgttgata acgtaggcct gacggctaga
     2581 cacctgacat tttttgaaat gctgggcaac ttttcctttg gtgattattt caaagaagaa
     2641 gctattgcct gggcatggga gctgttaaca gacgttttaa aaattgaaaa ggacagactt
     2701 tgggttacgg tatatttgga agatgatgag gctgagaata tttggcatac aaaggttgga
     2761 atacctatgg aaagaatagt aaggctggga aaggacgaca acttctggga actggaggtc
     2821 ggtccaagcg gaccctgctc ggaaatttac tatgacaccg gagatgacag aggctgcggg
     2881 tcacccgact gcaaaccggg atgtgactgc aacagatata ttgaaatttg gaatcttgta
     2941 tttacgcagt ttgacaagga cgaaaaaggt gtttatcatc cccttccgaa tccaaatatt
     3001 gatacaggca tgggacttga aagagtggct gctgtacttc aggataaacc cactgtgttt
     3061 gatgtggaac ctttgaattt aatagtaaac aaggcttcgg aaatttgtcg gattccctat
     3121 ggccaaaaca aaaaaagtga cattgcaatg aaaataatct gcgaccactc tagagcagcg
     3181 accttcatga taggcgacgg agtaattcct tcaaatgaag gaagagggta tgttttaaga
     3241 cgtttaataa gaagagcaat ccgcaggggc aagtcattag gaataaacgg tgaatttttg
     3301 accgggactg ccaaagtggt tatagataac tggaaggagg cttatcctgt attatcggaa
     3361 aaagaatcct acattttaaa ggttttaagc cttgaggaag aaagatttaa aaaaaccatt
     3421 gaccaaggtc tggaactttt aatgaaagaa atacaaatat taaaagaaac tgataaaaaa
     3481 gagctggagg gcaaggttgc tttcaagctt tatgacacct atggctttcc gtcagaactt
     3541 acggaggaaa tacttaagga aaacggattt agcctaaata aagaacagtt tatcaaagtg
     3601 atggaagacc acagacagat ggcgagagat gccagaaagt caaccggtgc ttcgggctgg
     3661 aaggaaaatg attttactat agatacggat aaaaccgtat ttacaggtta caataattta
     3721 gaagattctt ctgaagttgt tgctatagta gtcggctcag aacaggtaaa tacaatcaat
     3781 gaaggtcagt caggaataat tgtggttgac aaaaccccat tttatgctga gagcggcgga
     3841 cagaccggcg ataggggaga atttattgct gatgacatgc tggcaaaagt aacagatacc
     3901 cataaactca ataatgatat ttttctgcat gaaacaaatg tggtaaaagg aagtatttca
     3961 gtgggagatt ccataaccgc caaggtagat gcagacagaa gaaaaaatac cgcaaagaat
     4021 cacacctgca cgcatttgct gcacagggtt ttaaggcaaa tgctgggaga tcatgtaaat
     4081 caggcaggct cgtatgtttc agaagacagg cttagatttg actatacaca ttttgaagcc
     4141 gttgaccata aaacattgaa ggaaatcgaa aaaagagtca atgaagctat attggcagac
     4201 tacatggtca gaactgatat actaaatatt gaagaagcta aaaagtcagg agctacagct
     4261 ttgttcgatg aaaaatacca ggaaaaggta agggttgtca gtgttggaga tttcagcaag
     4321 gaattgtgcg gcggaactca tatagatgaa acagccaaaa taggattgtt taaaattgtt
     4381 tctgaaggaa gcatagcttc aggaataaga agaatagaag caataacagg aagagcagca
     4441 ataaattact tacgggaact ggataattta acggatgaat tatccatatc catgaaaacc
     4501 accaaagatg aactgttaaa caaagttaat atgcttaaaa aagaagccaa ggataaagac
     4561 aaggaaatac aaaagctgaa caatgaactg ttaaggggaa atatcagtga aattcttgac
     4621 aaatatgaag aaattaacgg tatcaaactg tttgcgcttt accttaaaga caaggatgcc
     4681 aacacattaa gggaaattgc cgatagaatc aaggacaaaa atgaaagctg tgctgtaatt
     4741 ttagcttctg acctgggaga caaagtatta tttgtatctg ccgtaaccaa ggatttggtt
     4801 gaaaagggag tacatgcggg aaatatggtg aaagaagctg ccgtgattgc aggaggcggc
     4861 ggaggcggaa gacctgactt tgcacaggca ggagggaaaa accccgggaa aatagacgaa
     4921 gcaatagagg cagtgaaaaa acaattatcc atgaacaaat agttacaaag tgacattctg
     4981 agctttcgtt cactccgggt gtgaattgta atgatacggc tttgtaatta ggcgagtgtg
     5041 caaaattcga ttagtgcacg gcaaaactgc tcatatttat atagaataat gaattaaacg
     5101 aaaatttgca cacttcaatt agattatggg tgatgggtga gaattaatat tattaaaagt
     5161 tcaattctaa gaatttcagg ttttaattga ttttattaat gtattatagt acaatgataa
     5221 gagatgttaa ggggtggttt ttatggaaag taatacaaat aacactgcaa aatttgacta
     5281 cagtatgggt tcgcttaatg aagccaggga aatactatcc caggtttaca agtcacttaa
     5341 agaaaaggga tacaatccta acaaccagct cattgggtat atattgtccg gagatcccac
     5401 ctatattaca aatcataaca atgcaagaag cttaatacga aaaatagaaa gagacgaact
     5461 tttggaagaa attttaggcg tttacctgga tgcaatcgac ttatagactg gggatacagg
     5521 aatgtccccc tacttggggc acacaacttt gagcagtata taaatatact gcttttgtnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt ccccctactt ggggcacaca
     5701 actttgagca gtatataaat atactgcttt tgtgtgtctt gggtatagcc ctcagaaaca
     5761 gacggtagta ataatatgaa atacataaat ttatttgaaa aaagaaatgt ttcaaagctg
     5821 tgttacggaa ccctttcttt aagcaatctt caaaattcag ataccctgga aaacagggtg
     5881 agacttctta attattcatt agaaaaagga ataaactttt ttgacacagc tgagctttac
     5941 aataactatg atatactaaa agaattcata aaaggcaaag acaggaataa acttgtcata
     6001 gcatcaaaaa gttacgcata tgacaaaaat accgcagaat acagcataaa taaggcattg
     6061 aaagaaatgg atacggatta tctggatgtt ttcatgctgc atgagcagga caacggtaac
     6121 aattttattg gtcactataa agctgttgac cgtttcatga aatataagga agatggaata
     6181 ataaagcact ttggaatttc cacccacagg gtggaggcgg taagagattc aattaagttt
     6241 aaagagatag aatttatttt tcctttgttt aattacaaag gtatcggaat tcaggacggt
     6301 aatatcgaag atatgtctga agccttgaag aaggcaaaag aatacaataa atttattatg
     6361 gccatgaaaa tctatggcgg aggtcattta ataaatgaag cagaaaaagc ttttaaatat
     6421 gttgacaatt taaattatat agattccata gcagcaggta tgggcactgt tgaagaaatt
     6481 gatgcaaata tctcttatat ggacaacaat gaattaaatg aaaacattaa aaataaaatt
     6541 aggaatcaaa agcgcactct cgtagtagaa gagtggtgtg ccggatgcgg aaaatgcgca
     6601 aaaaaatgca ggcagaatgc acttgaaata gttgacggca aatgcagggt tgatatggaa
     6661 aagtgtgttt tatgcagtta ttgcgccggt gaatgcaaag atttttatat caaaattgtg
     6721 tgaggtttct atggaaagac tgatgggatt ggatgtgggc gataaaacaa taggggtagc
     6781 tgtaagtgat ttattgatga ttactgcaca gagtgttacg acaataaaaa gaacaaacat
     6841 taaaaatgac ataaatgaac tgaaaaagct catcaatgaa tatcaagtta ctaaaatagt
     6901 gttaggcata ccaaaaatgc ttgatggaac tattggaata cagggtgaaa aggtccttaa
     6961 ctttttggaa aagatgaaaa ggtatattga cctgcccgta gaacttgaag atgaaagatt
     7021 taccacaacc atatcagaaa gaatgctgat agaagctgat gtaagaagaa aaaaaagaaa
     7081 agaagttatc gacaaattag ctgcagcaca aattttgtca tcttatatgc agcgtacaaa
     7141 ataatcaaaa ggagagccaa tggataatat tattgaattg ataaatgacg aaggaaattt
     7201 agaaaaactg gtcgtagaag tcacctttca tattgatgat attttgtatg cagtacttca
     7261 caaaatcaac tcggatgaag gtatgattta ttatgtggaa gaaatggaag acggaagtat
     7321 agtgctgatt aaggttgagg acgaggatga aatcaaagaa gtgcaggaaa tttacgaagg
     7381 aatagctgat gatttgattt agagcttaca ggggaattta tgtcagaaga aatcattgaa
     7441 aaacaataca attttgaaat attaaatcat gcggtcattt tcaccggaat ctgtgcacaa
     7501 tgcagggata aggagaatta agatggcaga aaagaaacaa aagctcaaaa taataccctt
     7561 gggtggattg ggtgaaatag gtaaaaactc cacagtcata gaatataaca acaatataat
     7621 tgtcatagac tgcggaatgg ctttcccagg cgatgaaatg cttggtattg acatagttat
     7681 accagatata acatatttaa taaaaaacaa agaaaaaata aaaggaataa ttttaactca
     7741 cggccacgaa gaccatatag gttcaatacc atatactcta aaaaaactga atgtacctat
     7801 ttacggaact aagcttacat taggtcttgt ggaaaacaaa ctgttagagc ataaaataga
     7861 agatgcattc ctaaatccga tcaaagcagg agacaccttt gatttaggat gctttaaaat
     7921 tacagctgta agagttaacc acagcatacc tgatgcagtg gcttacttta ttgatactcc
     7981 aatcgggacg gtagtgcaca ccggtgactt taaaattgat tacactccaa tcaatgaaga
     8041 ggttattgat ttaggaaagt ttgcggatat cggacgaaaa ggcgttcttc ttgccatgtc
     8101 tgacagcact aatgttgaaa gactgggatt tacagaatcg gaaaaaacaa tcggccaaaa
     8161 atttatggaa atatttaaac actgtgattc ccgaataatt gttgcctcct ttgcttcgaa
     8221 tatccacagg ctgcaacagg ttatcgatgc tgcggtattt aacaaaagaa aggttgcaat
     8281 atcgggaaga agcatggtaa atgcaatcga ggtttcactg gatttaggat atttgcatgc
     8341 tcccgaggga acaatcataa atgttaacga ggtgaaaagc cttaaggaca atgaaattgt
     8401 tattcttacc acagggagtc agggagagcc tatgtctgcc ttgacaagaa tggctaacaa
     8461 tgatcataaa aaaatccaga taaaacccgg agatctggtg gtaatttcag ctaatcccat
     8521 accgggaaat gaaattacag tttcaagagt aatcaatatg ctgtacgaaa agggcgcaag
     8581 ggtactttat gatgcattga ctcaggtaca cgtatcaggc cacgccagaa gagatgaact
     8641 gaagcttatg cttacacttt taaaaccgaa gtttttcata ccggttcacg gggaatacag
     8701 acatttggta cagcatgcta atctggctaa tgaattggga atgcctattg aaaataccat
     8761 aataggaaat aacggtgatg ttattgaact tacatccaag tcaatagtaa aaaacaatac
     8821 tgttacatcc ggaaacatac ttgtagatgg attaggcgtg ggagatgtgg gcaatattgt
     8881 tcttcgggac agaagacttc tttccgaaaa cgggttgata gtagttgttt tatctacaga
     8941 aagaggcaca ggaagaatac tggcaggtcc tgaaatagta tccagaggat ttgtatatgt
     9001 gcgtgagaat atagacctga tagaggaatc taagacagtg attaataagg ccttggaaaa
     9061 atgtcaggat acaaatgtta aagaatggaa caatataaaa atggtaatta aggattcatt
     9121 gagcagcttt atttatgaca aaataaaaag aagccctatg atactgccga taatagttga
     9181 agtaaatcca gatgaataat gagttagatg agggacacat tcgtgtccct tggttttatg
     9241 tcattgctat gaataagcga tgtgtcactc tgatagcttt agctcgaaag atgccctaac
     9301 cccatttttc agagtattgc gaaatctcag ctttttacgg gagtcagctc tgatgcatcc
     9361 gtaaaacaat accaccacgc aagacagtta agatctaagc tttcagagtc cgtgcctctt
     9421 gcaattgcct cgtcaagtgt atttgcaacc ggaacaataa ccggatcacc cggcatccag
     9481 tttgcaggag tactcacacc atatgtagct gatacctgta gtgcatcaat caatcttaat
     9541 aattcataca tgtttctgcc gtttgtaccg ggataagtta aaattgctct tattctctga
     9601 ttagggtcta ttatgaaaac atctctgaca ctttgctcat aaattctgtc aggattaacc
     9661 atgccgtaaa gcgctgatat ttgtgcgttt ctgtcagcaa caagaggaaa gggcattact
     9721 atgccgttca tttgaaagat atcataaagc cagccgatgt gtgcaggatt tgagtcaatg
     9781 ctaatgccta aaagctgaac atttcttttt tcaaattcag gttgaagctg tgcaaatgcg
     9841 ataaattcag atgtgcaaac aggggtgaaa tctcccggat gactaaaaaa caaaacccat
     9901 tttcctctat actgggacaa agttattggt ccctctgtag tcatagctgt aaagtcagga
     9961 gcaattcttc cgattgtcaa gcatatcctt gtacgttcgg tttctatatt atcattactt
    10021 atcataatta attgaggtta cctcccattt gtatttagtt tattatatga gagggagtgg
    10081 agaatggtga aaaacgcagc ctaattcgct ataatatttg tttcctttgg tcgcgtaaaa
    10141 acaaccggtg agcttttagg tcaccggtta ttaacgctta ttatcttttg atgttgtgaa
    10201 agttaacgcc tagaataggc ccgaactgag atggtttgtt attaacaata cttttatctt
    10261 ccattctttg atttgatttc catgaaatca catttagatt tggagttagt ttaccgttta
    10321 tatcactggt caacttgtat tgcccatccc ttcttagttt aatcacgtct gaattaacaa
    10381 ctctggttaa atcgggagtg ctacttttgt tgaatctgtc caatacaatc aaccctctct
    10441 tatccaaaag taataagcaa tattatatta accagccaaa caattattat actattaaat
    10501 tttaaattat tgtaattaat gatcaaaggt tgtataattt aatggataaa attaatcagt
    10561 ggaggtatta tgaaaaagat aatagcacta ctatgtgttt tggttataat agcagcagca
    10621 tatctttatt taaataatta tattgtagaa agtttgacag ctgttgatat aaacgataag
    10681 acagcaataa ccgttgaaat tccatcaggc agcacaacca atgacattgc tgaaattttg
    10741 tttgaaaaca atttaatctc aaatattcgc acatttaagt attatgctga aagaacaggt
    10801 tcagattcaa aattaaaagc aggaagctac gtactttcaa gaaatatgaa tgctgatgaa
    10861 cttttggatt cccttattaa gggaggatct tcaggaaata cttataatat tacaataatc
    10921 gaaggactga ctaacgaaga catggctcag gtcttgtccg agcagactgg gctaagttat
    10981 gacaaattca ttgaacttat ggaagctcct gaaactttca gggaagatta tgaattttta
    11041 aatgatacat ctgtttcgaa tctgcaggga tatttaatgc ctgataccta caatatttat
    11101 attgattcat cggaagagga tataataaga gttcttttaa aacaatttga tgaattctat
    11161 ttggatgaaa tcaagccaag gatgggaaat actggtcttt cctttgaaga ggttataaat
    11221 ttggcaagca tagttgaaaa ggaagccctt ttggacgagg aaagagatga agttgcaaaa
    11281 gtatttctaa accgccttga cataaatatg aagcttcaat cctgtgctac cgtaaactat
    11341 gcaaacgggg agtggaagga aagacttaca tatgaggata ttgaaattga ttccccttac
    11401 aacacttata tagtggaagg actgcctcct gcacccataa attcgccggg cagggtatca
    11461 ataatatctg tacttgagcc tgcagatgtg gattatttgt tctttgtggc gaagggagac
    11521 gggtcccact atttctcaaa cacctatgat gagcacatag acgcagctga ggagtatctt
    11581 aactaactat ggacaatata aaccgtgaat atatagataa gtatattaac agtctgatac
    11641 ctgtagataa tgaaaaatta aatgaattca gagaatactg cgaagaaaga aatttgccca
    11701 ttatccataa agaggtagga caatatatta aacttgttat aaatcagctg aatgcaaaaa
    11761 gtatagttga aataggcaca aacgttggtt attctgcaat attcatgtct aaggtaatga
    11821 agggcgaagg cagggttgta acctttgaaa gaagcgaaaa attttataag gaagcaataa
    11881 aaaacattgc tgatttcgga ctggaaaaca atattgatgt tcatttgggc gatgcagtgg
    11941 atattttgaa tgaaacagaa ggaacttttg atatggcctt tatcgacgcg gctaaaagct
    12001 attacagggt attctttgat aaatgcttaa aaatgatgaa gcctggagga attattcttt
    12061 cggacaatgt attgtaccag ggaatgattg catcggatga attagttgta agaagaaaga
    12121 aaacactggt aagaaatttg agaaactatt tagaatatat atctcatgat aatagatttg
    12181 taacttcaat tcttcctctt ggggacggat tggcggttac atatataaag gattaaggag
    12241 tgaatatgtt accaaaatta ttggcacccg caggaaatct ggacaaattg ataatggctg
    12301 tgcaatatgg agctgatgaa gcttattttg cgggaaaatc aatgggaatg agggcaggag
    12361 gcaaaaactt cactaaaaag gacttggagc tgggagtgga gtattgccac aaatatggaa
    12421 gaaaagcaaa tataacagtt aatatagtgc cccacaacga tgacttgaag ggtattgaag
    12481 gatatttgaa atatttgcac aaaatcggca cggatgcatt gattgtagca gaccccgggg
    12541 ttgttgaaat tgtaaaagaa acaattccgg atatgaggat tcacttaagt acccaggcta
    12601 ataccacaaa ttaccacaca gcaaatttct ggtacaaaca gggagtcaac agaattgtgc
    12661 ttgcaagaga gcttaccttt gatgaaatag aaacaataat aaataacaca cccgatgacc
    12721 tggaatttga aacatttgtc catggagcca tgtgcatatc atattcaggc aggtgtctgc
    12781 tgtcaagctt tatgacagga agattttcaa acaagggaga ctgtgcccag tcctgcaggt
    12841 ggaaatacaa cctggtcgaa gaaaaaaggc ccggtgagta ttaccccata gaagaaactg
    12901 atgaaggaac atttataatg aattcaaagg atatgtgcat gatagagcat ttgcagaagt
    12961 tcaaggactt aggcatcaaa gctttaaaaa ttgaaggaag aaataaaagt gaatactata
    13021 cggctgtaac agtccgctcc tatagaaatg cactggatga gcttgaatat cctgaaagtg
    13081 aaaggaatac ctcctactgg aaggaagagg ttgagaaaac atcacacagg gatttctcct
    13141 atggattttt cttcggcaat ccatacaagg atggtcaatc atatacaaac tcaagctata
    13201 tcagaaccta tgatgtggta ggaataataa gatcctatga ccagacatca aagactgcta
    13261 ctgttgagca gagaaataaa ttttcagagg gcgacctttt ggaatgctta ggtcccaaag
    13321 gaaaacattt tgaactgaca gcaagaaatt taacagatga agacaataac aaaatagatt
    13381 ctgcacccca tcctcagcag acagtaaaac tttatatgga agaaagtgtg gaaccatact
    13441 atattttaag aaaaaaagtt taattactag ggaacggtac tactgtcatt tgacagcatt
    13501 accgttccct tgacaacctt gccaggctag tttcctaagt tcgagaccaa ggcgtcatgg
    13561 agttttttaa gggctttttt ttcaatcctt gacacgtaag
//
LOCUS       DAOPUT010000044        12205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_5306_length_12205_cov_20.841399, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000044 DAOPUT010000000
VERSION     DAOPUT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5306_length_12205_cov_20.841399"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..2916)
                     /gene="uvrA"
                     /locus_tag="PKK75_05280"
     CDS             complement(106..2916)
                     /gene="uvrA"
                     /locus_tag="PKK75_05280"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNZ83044.1"
                     /translation="MDKIIIRGAKENNLKNISLELPRDKFIVFTGVSGSGKTSLAFDT
                     IYAEGQRRYVESLSSYARMFLGQMDKPDVESIEGLSPAISIDQKSTNRNPRSTVGTIT
                     EIYDYYRLLFARIGVPHCPQCGKPITTQTIDQITDKVLEYEERTKIQVLAPMVRGAKG
                     THKKLLDDIKKEGFVRVRVDGELYELEDDIVLEKNKKHTIEVVIDRLVIKDGIQKRLV
                     DSVETALSLSGGMVLIDVMGQEELLMSTKFACTDCGIGFGEISPRMFSFNNPIGACET
                     CNGLGVKREIDVDLVIPDYSKTIEERAIAPFGTEEEGYYYQVFAGLAKHHKFDMSKPL
                     SQAPQEFIDELLYGTKGREVKVNFVSKFQGPREYFREFEGVVNNLQRRYNETRSPEMR
                     QWIEEYMYESPCPACNGARLNPISLAVKIDNKNIYEVTQMSVVKSLQFFDQLNLSPMN
                     KIIGEQIIKEIRDRLQFLSDVGLNYLTLARNASTLSGGESQRIRLATQIGSSLVGVLY
                     VLDEPSIGLHQRDNAMLIKTLRSLTDLGNTLIVVEHDEETMQSADYILDMGPGAGIHG
                     GEVVEFGTIDTIMNNPNSITGQYLSGKKKIQVPQVRRRAEKFIEIKGARENNLKNLDV
                     KIPLGIMCCVTGVSGSGKSSLINEILSKGLHQKLFKNTNRPGKFDEMQGIENVDKIIT
                     IDQSPIGRTPRSNPATYTGVFDYIRDVYSMTLEAKERGYKKGRFSFNIKGGRCEACHG
                     DGILKIEMHFLSDVYVPCDVCKGKRYNRETLEVKYKGKNIADVLDMTIDEALDFFENI
                     PKIQNKIKTLQDVGLGYVKLGQPSPELSGGEAQRVKLAYELSKRGTGKTVYVLDEPTT
                     GLHTADIHMLTEVLDRLVEAGNSVIVIEHNLDVIKTADYIIDLGPEGGDGGGLIVAEG
                     TPEEVAKVDGSFTGQYLKKVLQ"
     gene            complement(3068..5041)
                     /gene="uvrB"
                     /locus_tag="PKK75_05285"
     CDS             complement(3068..5041)
                     /gene="uvrB"
                     /locus_tag="PKK75_05285"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNZ83045.1"
                     /translation="MGEFKIKSEYVPTGDQPEAIEKIARGLKKNYKYQTLLGVTGSGK
                     TFTMANIIEKVQKPTLVLAHNKTLAAQLCSEFKEFFPDNAVEYFVSYYDYYQPEAYVP
                     SSDTYIEKDSSINDEIDKLRHSATAALFERKDVVIVASVSCIYGLGSPVDYENLVISL
                     RPGMIKDRDEIIRKLVEIQYKRNDIAFTRGTFRVRGDTVEIFPASSSENAIRIEFFGD
                     EVDRITEINTVTGEMLGRRNHISIFPATHYATTEENVNRAVESIKVELQERLKKLESE
                     NKLLEMQRLEQRTNYDIEMLQEMGYCNGIENYSRHINNLQPGARPYTLLDYFPKDYLM
                     IIDESHMSIPQVRGMYNGDRSRKTTLVEYGFRLPSALDNRPLKFDEFENIINQAVFLS
                     ATPGPYEIEHSQNIAEQIIRPTGLIDPPIYVRPVENQIDDLISEIRKSTDKNQRVLVT
                     TLTKKMAEDLTDYFKSTGIKVRYLHSDVETLERMEIIRDLRIGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLIQTVGRAARNIDGRVIMYADRMTGSMERAIRET
                     DRRREIQDRFNKEHNITPKSVIKGVRDVIEATRAAEEEGKYKTKGKDISLFEYIIELE
                     KEMRKAAENLEFEKAAHYRDEIRELKKYGDIPE"
     gene            complement(5149..5640)
                     /locus_tag="PKK75_05290"
     CDS             complement(5149..5640)
                     /locus_tag="PKK75_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ83046.1"
                     /translation="MKILVISDSHNVILDSQINDIKREGQFDLLIHCGDKYNDAEKFA
                     EKLNIEGVLNVPGNCDYNYNITGTKFNIVQDIEGKKFLITHGHTHNVKMELGRLKDYA
                     KKYKVDIVVYGHTHKSQNEIIDNILFFNPGSTIFPKDGRASFGVIEITNDHFNSKIVV
                     LEN"
     gene            complement(5615..6235)
                     /gene="rdgB"
                     /locus_tag="PKK75_05295"
     CDS             complement(5615..6235)
                     /gene="rdgB"
                     /locus_tag="PKK75_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNZ83047.1"
                     /translation="MNRRIILSTGNAHKIDEIKGILKNMPFELISKNDLGYEDFDVVE
                     DGSTLEENALKKAEELHKLTKGIVIADDTGLYVDALNGEPGVYSARYAGEHAAYADNN
                     KLLLKKLKDVSLEKRTAYFKTVIAVILEDGSRLTAEGICRGHIALQERGKRGFGYDPL
                     FVPDGKDKTFSEMSEEEKNKISHRANALINLKDKLEALFEDIGDKR"
     gene            complement(6228..6974)
                     /gene="rph"
                     /locus_tag="PKK75_05300"
     CDS             complement(6228..6974)
                     /gene="rph"
                     /locus_tag="PKK75_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNZ83048.1"
                     /translation="MNRTDNRLSNELRKVEIHRNYLMHPQGSVLIETGNTKVICTAMI
                     EDKVPPFLKGTGKGWISAEYSMIPGSTAIRKARDINKGKIDGRSQEIQRLIGRSLRSV
                     VDLEGFGERTMWIDCDVIQADGGTRTASITGAFVAMTEAFHKLKHEGLIEGFPVKAFV
                     SAVSVGILNDKPILDLCYEEDSGAEVDMNIVMTDEGKFIEVQGTGEETTFSHDQLMEL
                     ITLAAKGCHDLYQIQKNTLGEEISREISNE"
     gene            complement(7212..9314)
                     /locus_tag="PKK75_05305"
     CDS             complement(7212..9314)
                     /locus_tag="PKK75_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HNZ83049.1"
                     /translation="MMRIKYFKWPIFISLLLIITLIQYSAPDAYAEDDSIRIVIDGKR
                     IKSDVEPYIKNDRALVPVRVIAEELDSVVEWDNDNRAVHISKEDTHILLRIDSYLVEY
                     TNDNETTYAVIDVAPEITGDRTFVPIRLISNALGVGIEWDNERRAVLIDSSESSKVEK
                     FFDVEISSVSSGQTITGITDLYTDTVDEQPKGAVEIKYLLLDRDTAKGFVVASGDIDK
                     THKWVPAMEDNGKKILVAAFYDAEGNFLAGDSIPVTVKIDPRIELKGIREGQLITAHS
                     VPLSTSLNFSAAYVKYEMINPDNGAVYLSPGVDPEQSFTMIPVIEDNGHMSVRVIAYD
                     TKGNAYPGQYVNIGIDVKRYLNLGGVKNGQTIDGSVTLLAQRNFNVTDTEYYIIDNAT
                     GRESLLFKAAYGSYSWFPGPEDAGSKDLYVKVKDTAGNTHLSSRVGVNVTGSPKFLLQ
                     GVGPGQVLTEAAKFNVKTNVVELDSIKYILTNSVTGQERVISEERNPTFTPDAGDSGS
                     WSLRAEGTYGGKTFKTEEVKFTIYTGELYPAKPVIEKDLYLDLVSGLAVNTRKTYGMS
                     AALQTAQAILETGWGQKVPVDKYNGNFSYNLFGIKGSGTKGSVISSTWEEYNGVAFTI
                     DAEFRAYNNEKESWQDHKDLLLLRERYAPFREVMHDSTKGAWALKRCGYATDSQYAIK
                     LIDIINRYGLRELDEVTI"
     assembly_gap    9526..9625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9626..9909)
                     /locus_tag="PKK75_05310"
     CDS             complement(<9626..9909)
                     /locus_tag="PKK75_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtrH family sporulation protein"
                     /protein_id="HNZ83050.1"
                     /translation="MSSFYSSIVQIFLIAFGVVTGASLFSGLGAVLTNTPPLKAMTDV
                     AGSIKIWAIAASLGGTFSSFAMIEKGLFEGEIRTLIKQVFYILISLTGANT"
     gene            complement(9987..11984)
                     /locus_tag="PKK75_05315"
     CDS             complement(9987..11984)
                     /locus_tag="PKK75_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HNZ83051.1"
                     /translation="MMTDSNDEALRMLDEEIRYLKLLSKQYPTVASACTEIINLQAIL
                     NLPKGTEHFLSDIHGEYESFNHVLRNGSGNIKRKINEVYGDIMSEKEIKSLATLIYYP
                     EQKLEIIRKGEEDLEDWYRITIYRLVVISRKAAFKYTRMKVRKALPKEFAYILEELMY
                     KGPEEDKEEYYSEIINTIIKIGRADEFIIAMAKLIQRLVIDRLHIVGDIFDRGPGPDK
                     VMDTLMNYHTVDIQWGNHDVLWMAAAAGSEASIATVLRICARYANLDIIEDGYGINVL
                     PLATFAMEYYGDDECLQFKPRVESDIVYSDKDLKLIAKMHKAIAMIQFKLEGQIIKRR
                     PHFNMEDRLLLDKINHKEGTIEVYGKTYPLNDTNFPTVDPDNPYTLTPEEEELVERLK
                     SSFLNSEKLQKHIRFMLTNGSMYLKFNSNLLYHGCIPVNEDGSFKKVQIGQSGNSYSG
                     KAYFDRLEILVREGYFHKTNPEAKQYGMDITWYLWTGADSPLFGKDRMTTFERYFIDD
                     KETHKETKSYYFKLEEREEMCRRIFKEFGLDPECSHIINGHMPVKSKSGESPIKANGK
                     LIVIDGGFSKAYQGTTGIAGYTLIYNSYGLLLVSHNPFESTQKAIEKEKDIHSTSMVL
                     EKELERKRVKDTDAGKVMKSQIKDLEMLLDAYRLGFVKEQE"
BASE COUNT         3029 a         2751 c         2062 g         4263 t
ORIGIN      
        1 ttaagatgtt gtttttcttt tcacccagcc tcattttctt gttaaagtca atgaaaggtc
       61 caaatttttt tcgctatcgc tgaatgaaga atattgagct ttcaattact gcagcacttt
      121 tttcaagtat tgtcctgtaa aggacccgtc tactttggca acttcctcgg gagtaccctc
      181 tgctacaatt aagccgcccc cgtcccctcc ttctggaccc aaatcaataa tgtaatctgc
      241 agttttaata acatccagat tatgttcgat tacaatgact gaatttcctg cttccacaag
      301 tctgtccaat acttctgtaa gcatgtgaat atcagctgtg tgaaggcctg tagtaggttc
      361 atccaggaca tagactgttt tgcctgttcc tcttttgctc agctcatatg ccagcttcac
      421 acgctgtgcc tcgcctcctg ataattcagg tgaaggctgg cccagcttca catatcccag
      481 tcccacatcc tgcagtgttt ttattttatt ctgaattttc ggaatgtttt caaagaagtc
      541 caaggcttca tctattgtca tgtctaaaac gtctgcaata tttttgccct tgtatttaac
      601 ttcaagtgtt tctctgttgt acctctttcc cttgcaaaca tcacagggca catacacatc
      661 agaaagaaaa tgcatttcaa tttttaaaat tccgtcgcca tggcatgctt cacatcttcc
      721 gccctttata ttgaaactga atctaccctt tttatagcct ctctccttag cttcaagggt
      781 cattgaataa acatccctta tatagtcaaa tactcctgtg taagtggcag gattggacct
      841 tggagtcctg ccgatgggag actggtcaat ggtaataatc ttatctacgt tttcaattcc
      901 ctgcatttca tcaaatttcc cgggacggtt tgtatttttg aataatttct ggtgaagacc
      961 ctttgacaat atttcattaa taagggagct tttgcctgaa cctgaaactc cagtaacgca
     1021 gcacattatc cccaatggaa ttttcacatc cagattcttc aggttgtttt cccttgcacc
     1081 cttaatttca ataaacttct ctgcccttct tcttacttga ggaacctgga tttttttctt
     1141 cccgctcaaa tattgtcccg ttattgaatt tgggttgttc atgattgtat caatcgttcc
     1201 gaactcaaca acttccccgc cgtgaatccc ggcaccgggc cccatgtcca ggatgtaatc
     1261 tgcgctttgc attgtttctt cgtcgtgctc aaccacaata agcgtgtttc ccaaatccgt
     1321 taaagaacgg agggttttta taagcatggc attgtctctt tggtggaggc ctatgctggg
     1381 ctcatccaat acatataaaa cacccacaag gcttgaacct atttgggttg ccagtcttat
     1441 tcgctgagat tcgcctccgg acagtgttga tgcatttcgt gccagtgtga gataattaag
     1501 tcccacatcc gataaaaatt gcagtctgtc ccgaatttct ttgattatct gttctccgat
     1561 aattttattc atagggctca agttgagctg gtcaaaaaac tgaaggcttt ttacaactga
     1621 catctgtgtc acttcatata tgtttttatt atctattttt actgcaagac ttatagggtt
     1681 cagcctggct ccgttacatg caggacaggg actttcatac atatattctt caatccattg
     1741 tctcatttca ggggaccttg tttcgttgta tcttctttga agattgttta caactccttc
     1801 aaattccctg aaatactccc tcggtccttg aaatttgctg acaaaattaa cctttacctc
     1861 tctgcccttt gttccgtaca gcagttcatc gataaactct tgtggtgctt gcgaaagggg
     1921 tttggacatg tcaaacttat gatgttttgc caggcctgca aatacttgat aataataccc
     1981 ctcttcttct gtcccgaagg gtgcaattgc tctttcttca attgtctttg aataatcggg
     2041 aattaccagg tcaacatcga tttctctttt aactccaaga ccattgcatg tttcacaggc
     2101 tcctatgggg ttattgaatg aaaacatcct gggagaaatt tctccaaagc ctatgccgca
     2161 gtctgtgcat gcaaactttg tgctcatcaa aagttcttcc tgacccataa cgtcaataag
     2221 aaccatcccc ccggacaatg acagggcagt ttccacggaa tccacaagtc ttttttgtat
     2281 tccgtctttt attacaagcc tgtcaatgac aacctctatt gtatgttttt tgtttttttc
     2341 cagaactata tcgtcttcta attcataaag ctcaccgtca actctcactc ttacgaagcc
     2401 ttcttttttt atgtcatcca gtaatttttt gtgagtccct tttgcacctc ttaccattgg
     2461 ggctaaaacc tgaatctttg ttctttcctc atattccagt accttgtctg taatctggtc
     2521 tatagtctgt gttgtaattg gtttcccgca ctggggacag tggggcactc ctattcttgc
     2581 aaacaaaagt ctgtaataat cataaatctc tgtaattgta cccacagttg aacgagggtt
     2641 cctgtttgtg gacttttgat caatggaaat ggcaggagac aatccttcaa ttgattccac
     2701 atcgggctta tccatctgtc ccaaaaacat tcttgcatag gaggacaaac tttcaacata
     2761 acgtctctgt ccttctgcat atatggtatc aaaggcaagg gaagttttcc ctgaaccgct
     2821 gactcccgta aaaactataa atttatctct tggaagttca aggctgatat ttttcaggtt
     2881 gttttccttt gctcccctaa taattatttt gtccattgtt tatcctttcg ctgattctca
     2941 aattggggac attcctcgtg cccgcaaaga cttatccctg aactaagagg tgtgtcccca
     3001 tacctattgt ggtgtgtccc catacctgtt ttggtgtgct cccgctcctg ttcagttgtg
     3061 tttccatcta ttccggtatg tctccatact tctttagttc tcgaatttca tctctgtaat
     3121 gggctgcttt ttcaaactcc aggttttctg cagccttccg catttctttt tccaattcta
     3181 taatatattc aaacagactg atatccttgc cctttgtttt gtatttgccc tcttcctctg
     3241 ctgccctggt agcttcaata acgtcgcgga ctcccttgat tacagactta ggagtaatgt
     3301 tgtgttcttt attgaacctg tcctgtattt cccttctcct gtctgtttcc cttattgccc
     3361 tttccatgga accggtcatt ctgtcagcat acattattac ccttccgtca atatttctgg
     3421 ctgctcttcc cactgtctgt ataagggatg tttcagaccg caggaatcct tccttgtctg
     3481 catcaagtat tgccaccagg gatacctcag gcagatccag cccttccctc aaaaggttta
     3541 ttcctacaag cacatcaaac tcgcctatgc ggaggtccct tattatctcc attctttcca
     3601 gggtttccac gtcggaatgg agatatctta cctttattcc tgtgctctta aagtagtctg
     3661 tcaaatcttc agccatcttc ttggtaagag ttgtaaccag caccctttga ttcttatcgg
     3721 ttgatttcct tatttcactt ataaggtcat ctatttgatt ttctacgggt cttacatata
     3781 tgggcggatc aataagtccc gtaggacgaa taatctgctc cgcaatattc tgagaatgtt
     3841 caatctcata gggacccggt gtagcactta aaaatactgc ctgattgata atattttcaa
     3901 attcatcaaa cttcagcggc ctgttgtcaa gagccgaagg aagtctgaag ccgtattcaa
     3961 ccagtgttgt ttttctggac ctgtccccgt tgtacatacc cctgacctga ggaattgaca
     4021 tatgagactc gtctattatc atcaaataat cctttggaaa ataatccagc agggtataag
     4081 gtcttgcacc cggctgcagg ttgtttatat gtcttgaata gttttcaatt ccgttgcaat
     4141 agcccatctc ctgaagcatt tcaatatcat agttggttct ttgctccagc cgttgcattt
     4201 caagcagctt attctcgctt tcaagttttt tcaatctttc ctggagttca acctttatgc
     4261 tctcaactgc cctgttcaca ttttcctcag tagtggcata gtgggtagca ggaaatattg
     4321 aaatatggtt tctcctgccc agcatctctc ctgtcactgt gttgatttcc gtaatcctgt
     4381 cgacttcatc tccaaagaac tcaattctta tggcattttc agaagatgaa gcggggaata
     4441 tttcaactgt gtcccctctt actctgaacg ttcccctggt aaatgctata tcatttcttt
     4501 tatattgaat ttccacaagc tttctgatta tttcatctct gtccttaatc attcccggtc
     4561 tgagggaaat aacaagattt tcataatcca cagggcttcc aagtccgtaa atgcaagaaa
     4621 cgctggcaac aatcaccaca tcctttcttt caaaaagtgc cgctgtggca gagtgtctca
     4681 atttatctat ttcatcattt attgatgaat ccttctctat atatgtatca ctgctcggta
     4741 cgtatgcctc cggctgataa taatcatagt agcttacaaa gtattcaact gcattatcag
     4801 ggaaaaattc tttgaattca gagcatagct gggctgccaa tgttttattg tgagctaaaa
     4861 caagggttgg tttttgtacc ttttcaatta tgtttgccat agtgaaggtt tttcccgagc
     4921 cggtaactcc aagaagtgtc tggtatttgt agtttttttt cagaccccgg gcgattttct
     4981 ctatcgcttc aggctgatcg ccggtaggaa catattcgga tttaatttta aattcaccca
     5041 ttataaggtc cttctttcaa aacgtatgtt cgttttatta tataactttt gatattccaa
     5101 gtcaacaaaa aagactatgg aggcaactct tgaagtaatg tcccgggatt aattttccaa
     5161 gacaactatt ttactgttga aatggtcatt tgtaatttct ataaccccaa agcttgcccg
     5221 tccgtccttg ggaaagattg tgcttcccgg attaaaaaat aaaatattgt cgataatttc
     5281 attttgtgac ttgtgggtat gaccgtatac gacaatatct accttgtatt tttttgcata
     5341 atccttcagc ctgcctagtt ccattttcac attatgtgta tgaccgtggg taattaagaa
     5401 tttcttccct tcaatatctt gtactatatt aaatttcgtg cctgtaatgt tgtaattgta
     5461 atcgcagttt ccgggaacat ttaacacacc ttcaatattt aacttttcag caaacttttc
     5521 tgcatcattg tatttatctc cgcagtgaat taataaatca aattgtcctt ccctttttat
     5581 atcattaatt tgtgaatcta aaataacatt gtgactatcg cttatcacca atatcttcaa
     5641 agagtgcctc caatttgtcc tttaagttta ttaatgcatt tgccctgtga cttattttgt
     5701 tcttttcttc ctcagacatt tccgaaaagg ttttatcttt tccgtcaggt acaaacaaag
     5761 gatcgtatcc aaaacccctt ttgcctcttt cctgaagtgc tatatgacct ctacagatgc
     5821 cttctgctgt cagcctgctc ccatcctcta aaatgacagc aataacggtt ttaaaataag
     5881 ctgttctttt ttcgagagaa acgtccttta gcttttttaa gagaagttta ttgttgtcgg
     5941 cataggctgc atgttctccg gcatatcttg cagagtacac tcccggctcc ccattaagtg
     6001 catccacgta taagcctgta tcatcggcga tgactattcc cttcgtgagt ttgtgaagtt
     6061 cttctgcctt tttaagtgca ttttcctcta atgtgctgcc gtcctcaact acatcaaagt
     6121 cttcatatcc caaatcattt tttgatatca attcaaaggg catattcttt aatatgcctt
     6181 ttatttcatc aatcttatgt gcatttcctg tggataaaat tattcttcta ttcatttgat
     6241 atttccctgc ttatttcttc tcccaatgta tttttttgta tttggtacaa gtcgtgacaa
     6301 cctttcgctg ccagggttat gagctccatc agctggtcat gtgaaaatgt agtttcttct
     6361 cctgtgccct gaacctctat aaactttccc tcatctgtca ttacaatgtt catatcgact
     6421 tcagccccgg aatcttcttc atagcataaa tcaagtattg gtttgtcatt tagaattcct
     6481 acgctgacag ctgatacaaa agcctttacg ggaaatccct ctattaatcc ttcatgtttt
     6541 aatttgtgaa acgcttctgt cattgcaaca aatgctcccg taatggatgc tgtccttgtg
     6601 cctccgtctg cttgtataac atcacagtct atccacatgg tcctttcgcc gaagccttcc
     6661 aaatcaacca cagacctcag gcttcttcct ataagtcttt gaatttcctg cgacctgccg
     6721 tcaatttttc ccttgtttat gtcacgggct tttcttatgg ctgttgaacc gggaatcatt
     6781 gaatattcgg ctgatatcca tccttttccc gttcctttca aaaatggggg caccttgtcc
     6841 tctatcattg ccgtgcatat tacttttgta ttgcctgttt ctattaagac ggagccttgc
     6901 ggatgcatca aatagtttcg atgtatttca accttcctta attcattgct taatctgttg
     6961 tctgttctgt tcatttaatt ctcctgtgta tttaaggggc acaccttctt aggattacac
     7021 tgccttattt taggtagttt aagaaaaaat caatagtgga atgtcccaat ttcaaacata
     7081 ttatatccca attggattta attaacaata agaattgtca taggaaaggg gacactcctt
     7141 gatacaaggt aaccctttgc gggtcatgct atcaaggaat atccccgaac tttttccctt
     7201 cactgcttta tttatattgt cacttcatcc agttccctca aaccatatct gttaataatg
     7261 tctatcagtt ttattgcgta ctgggaatct gttgcatatc cgcatctttt cagtgcccag
     7321 gcaccttttg tgctgtcatg catcacttct ctgaagggcg cgtatctttc ccttaagagc
     7381 aataggtcct tatggtcctg ccagctttcc ttttcattgt tgtatgccct aaactccgca
     7441 tcgattgtga aagccacccc gttgtattcc tcccaggtgc ttgagattac agaccccttg
     7501 gtgcctgaac ccttaattcc aaaaagattg taggaaaaat ttccgttata tttatcgaca
     7561 ggtacctttt gtccccagcc ggtttccagt attgcctgtg ccgtctgtaa tgctgcggac
     7621 atcccatagg ttttcctcgt attcacggca agtccagaaa ccaggtccaa gtaaaggtct
     7681 ttttcaatta caggtttcgc cgggtataac tcacctgtat aaatcgtaaa cttaacctct
     7741 tcggttttaa atgtcttgcc accgtaagtt ccttcagctc ttaaagacca tgagccggaa
     7801 tcacctgcgt ctggagtaaa ggttggattt ctttcttcag aaattactct ttcctgtcct
     7861 gttacagaat ttgtaagaat atatttaatg ctgtccagtt ctactacatt ggttttgaca
     7921 ttaaactttg cagcttcagt caaaacttgt cccggtccca ctccctgcaa cagaaacttg
     7981 gggctgcctg ttacattaac ccctaccctg ctgcttaaat gagtgttgcc tgccgtatcc
     8041 tttaccttta catacaggtc tttgcttcct gcatcttcag gtcccggaaa ccaggagtaa
     8101 gagccgtatg ccgctttaaa caacaagctt tcccggccgg ttgcattatc tattatatag
     8161 tattcagtat cagtaacatt aaagtttcgt tgtgccagca aggttacaga accgtcaatt
     8221 gtttgtccgt tttttacacc gcctaaatta agatatctct taacgtcaat tccaatgttt
     8281 acatattgtc ccgggtatgc atttccctta gtgtcataag caataaccct tactgacatg
     8341 tggccgttgt cttctattac gggtatcatt gtaaatgatt gttccgggtc aacccccgga
     8401 gacagataga cagctccgtt atcagggttt atcatttcat attttacata agccgctgaa
     8461 aaattcaggc ttgtacttaa gggcaccgaa tgggctgtta taagctggcc ttctcttata
     8521 cccttcagct ctatcctggg atctattttt acggtaaccg gtattgaatc tccggctaag
     8581 aaattcccct ctgcatcata aaatgctgcc acaaggattt tcttcccatt gtcttccatt
     8641 gcaggaaccc acttatgggt tttatctata tctcccgatg caacaacaaa gccctttgca
     8701 gtatctctgt ccaaaagaag atattttatt tctactgctc cctttggttg ttcatctaca
     8761 gtatcagtgt ataagtcggt aattccggta atagtttgtc ccgaacttac cgatgaaatt
     8821 tccacatcaa aaaacttctc aacctttgag ctttctgatg aatctatcag cactgccctt
     8881 ctctcattgt cccattctat tcctacgccc agggcattgc ttattaaacg tatgggcaca
     8941 aaggtgcggt ctccggttat ttcaggagcc acgtcaataa ccgcataggt ggtttcattg
     9001 tcgtttgtat attcaaccag gtagctgtca attctaagaa gtatgtgtgt atcctccttg
     9061 gatatgtgga ctgccctgtt gtcattatcc cactcaacta cagaatccag ttcttcggct
     9121 attaccctta cgggcaccaa ggctctgtca ttttttatat aaggctcaac atcggatttt
     9181 atccttttgc cgtcaattac tatccttatg gaatcatctt ctgcataagc atcaggtgca
     9241 gaatactgaa ttaatgtaat tatcaataac aaagaaataa aaatgggcca tttaaaatat
     9301 ttgattctca tcatccacct ctttcttttt ttacattata ccatgaaaat cgacataata
     9361 aacattaaca ttttattaca atataaccgg tcttccttta tgatgtaatc tcaaggggag
     9421 atacccttac ccacagcttt aaaatatccg acaggaccag tttatttact gtaagttgat
     9481 aatctgcgtt tttaaatctc agtatccttt tatctatgac ctgcannnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnngtatt ggcaccggtt aatgatatca atatataaaa
     9661 tacctgtttt atcagtgttc ttatctcgcc ttcaaataat cccttttcaa tcattgcaaa
     9721 ggatgaaaag gtgcccccca gggatgctgc tattgcccat atttttattg agcctgcaac
     9781 atcggtcatg gccttcaggg gaggagtatt tgttaaaaca gcacccagtc cggaaaacaa
     9841 gcttgcccct gtaaccactc caaatgcaat taaaaagatt tgaacaatgc ttgaataaaa
     9901 agaagacatt ttatcaccat actttcttta taaagtatga aatgtaaaat gtctttatga
     9961 ttaaccgccg ggatttatat cctgcattat tcctgttctt taacaaagcc taatctgtat
    10021 gcatccaaaa gcatttccaa atcctttatc tgactcttca taacctttcc tgcatctgtg
    10081 tccttaaccc ttttcctttc cagctctttt tccaaaacca ttgaagtgga gtgaatgtcc
    10141 ttttcttttt ctatggcttt ttgtgttgat tcaaaaggat tgtgagatac cagaagcagt
    10201 ccgtaggaat tatatatgag agtgtaacct gcaattccgg tagtcccctg ataagctttt
    10261 gaaaaccctc cgtctataac tatcagtttc ccgtttgcct tgatagggct ttctccgctt
    10321 ttgcttttaa caggcatatg gccgtttata atatgggagc actcgggatc cagtccaaat
    10381 tccttaaaaa tccttctgca catttcttct ctttcttcca gtttaaaata atagcttttg
    10441 gtttccttgt gggtttcctt gtcatctatg aaatatcttt caaaggtagt cattctgtcc
    10501 ttgccgaaca agggggaatc ggctcctgtc caaagatacc aggtgatatc catgccgtac
    10561 tgtttggctt ctggattggt tttatgaaaa tacccttccc ttactaggat ttcaagcctg
    10621 tcaaaatagg cttttccgct gtatgaattg cctgattggc ctatttgtac tttcttgaat
    10681 gaaccatctt cattaaccgg aatgcatccg tgatacaata gatttgagtt gaattttaaa
    10741 tacatgcttc cgtttgtcag cataaatcta atatgctttt gaagtttttc gctgtttaag
    10801 aaggatgatt ttagtctttc taccaattct tcttcttccg gagtaagggt ataggggtta
    10861 tcagggtcta ccgtcgggaa attagtatca ttcaagggat atgtcttccc atatacttca
    10921 attgttcctt ctttatgatt tatcttatcc aggagcagcc tatcctccat attaaaatgg
    10981 gggcgtcttt taattatttg accttccagc ttaaactgaa tcatggcaat ggctttatgc
    11041 attttagcaa taagctttaa atccttatct gaataaacaa tgtcgctttc gacccttggc
    11101 ttgaactgaa ggcattcatc atcaccgtag tattccattg caaatgtggc taaggggaga
    11161 acatttatcc cgtatccgtc ttctattatg tcaaggtttg catatcttgc acatattctc
    11221 aatacagttg ctatgcttgc ttcgctccct gcagcagctg ccatccacag cacatcatga
    11281 ttgccccatt gtatatccac agtatgataa ttcattagag tatccattac cttatccgga
    11341 ccggggcctc tgtcaaatat atcgcctaca atatgaagcc tgtctataac aagtctttga
    11401 ataagctttg ccatcgcaat tataaattca tcggctcttc ctatttttat tattgtgttt
    11461 attatctcgc tgtagtattc ttccttgtct tcctcaggtc ctttatacat aagctcttcg
    11521 agtatgtatg caaattcctt gggaagtgcc tttcttacct tcatgcgggt atatttgaaa
    11581 gctgccttcc tgctgattac caccagcctg taaatagtaa tcctgtacca atcctccagg
    11641 tcttcttctc ccttgcggat tatttccagt ttctgctcag gataataaat cagagtggct
    11701 agacttttta tttccttttc actcatgata tccccataca cttcattaat tttacgtttt
    11761 atattaccgg agccgtttct cagcacatgg ttgaaggatt catactcccc gtgtatatct
    11821 gaaaggaaat gttccgtgcc tttgggtagg tttaaaatag cttgaaggtt tataatttct
    11881 gtacatgcag aagcaactgt agggtattgc tttgataaaa gctttaaata tctgatttct
    11941 tcgtctaaca ttcgcagagc ttcatcatta gaatcagtca tgatatttct ccttttattt
    12001 atatttccac aacggatatt tacctatata aatattatat cataaagctt ataaatgtca
    12061 ttgatttttg aatttattat ataattatta aattttttat ttttttggta taaattaggt
    12121 aattttacag ataatatgaa aaacatatta aggaggttta taatgaaagg aataatgata
    12181 gttatagttg tagttttagt agtgg
//
LOCUS       DAOPUT010000045        11974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_5446_length_11974_cov_20.822301, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000045 DAOPUT010000000
VERSION     DAOPUT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11974
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5446_length_11974_cov_20.822301"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..571
                     /locus_tag="PKK75_05320"
     CDS             191..571
                     /locus_tag="PKK75_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83052.1"
                     /translation="MYEIRQQQRKQMREHRFFYHFILVIGIFVFAQGCQLMTKNPGYS
                     ASAAILGIMLQRHSVDKVFIRIFKFDPHKNAKVAMILLLCVIAVFSYFKRLRFIFFIL
                     LDLSSIILFAATAFIYQKLKTRQE"
     gene            575..2299
                     /locus_tag="PKK75_05325"
     CDS             575..2299
                     /locus_tag="PKK75_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004442306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ83053.1"
                     /translation="MLKRFISYYKPVKKIFIADMICAFAVAVCDLFYPMITRNIINIY
                     VPNQQLQLMITWLVVLGLIYILKFGLNYYITYYGHIMGIKMQADMRKDIFEHLQDLPF
                     VFFDENKTGNLSSRIINDLMDISELAHHGPEDFFISLVMLIGTFIMLSTINLPLALIV
                     FSIVPLLMFVTMKLRIKMEDAFMETRVTIGEVNATIENSISGIRVSKAFSNKENEINK
                     FNKNNSRFQEARRKAYKVMTEFYVGSSFIIDILNIVVLSAGAVFFFKGSINFGDFAAF
                     LLYIGNFLNPIRKLVNFVEQYQSGKSGFKRFAEIMNKEIEKDDPGAVDIYDVKGDISF
                     NYVTFAYDDNKEVLKDITFDIEAGKTVAFVGPSGGGKTTLCHLIPRFYEIEDGSISID
                     NMDIRRFTRRSLRKNIGIVQQDVFLFTGTILENIQYGRFDASREEVYEAAKNANIHDF
                     IMSLPEGYDTYVGERGVKLSGGQKQRISIARVFLKNPPILVLDEATSALDNATELLIQ
                     KALDKLSKGRTTVVVAHRLSTIKNADEIIILTDQGIQEKGKHQELLNKNGIYAGLYNS
                     QFIEEGEL"
     gene            2299..3027
                     /locus_tag="PKK75_05330"
     CDS             2299..3027
                     /locus_tag="PKK75_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ83054.1"
                     /translation="MKVTIVGHWGGFPKVKEASSGYLIEHDDYKILLECGSGVISLLQ
                     EKTDIKDLDAVLITHYHYDHCCDIGPLQYAVQIKTQLKEISKPLPIHAPDGEFFKLLK
                     WEDYTYGVSFDENSSLTLGPFEIRFIKNIHPVEAYSVRIKCRGKIFSFTSDTSYFEEL
                     AEFFSGSDLLICECSFYSDMDGTKAGHLNSSQAGLLAEKANVKKLILTHLPHFGELNN
                     LLTETMEYYKGDVVLASCLMEINL"
     gene            3112..3333
                     /locus_tag="PKK75_05335"
     CDS             3112..3333
                     /locus_tag="PKK75_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83055.1"
                     /translation="MIDVKGRRKCENCGKRYNYTYTKGEEASKKVKFKGKDENLSTVT
                     NVKVLSIHTYEVTARCPECGFEEIFIYND"
     gene            complement(3543..5357)
                     /locus_tag="PKK75_05340"
     CDS             complement(3543..5357)
                     /locus_tag="PKK75_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ83056.1"
                     /translation="MTKDYGKPIIAFIIALAIAFLVFITINNSFSLYESGQVFVLSDE
                     NEIHSLIGYREDLNTGKYYFDIVVEDTNVQLALIYGFITGDIKVNGDILVDSSNYNST
                     RFHSAAIDSRLFDVQTNSMTLEFNSKSNPYNQNVYLTTVNGAQSGISAYYMIFAFNIG
                     MTLLMSLYGASLYYHKPSEKYLIWFSIYTGALTLWSIGSLHMDTNSVIIKELVSHAYG
                     WCALLDIIICFKIFKTKLPGPFDKLLSGGGVAGMLIAWAFFETLLPNILKDYYYLFFF
                     SIGALIYACANKRKGAWLLLLGHTISLGMRLVVAYSSLNTMNVSFWLRVMRYSKSFNI
                     PFAICCLLLINRLFAEKFSESEALSAQLEQSNQVLEVKVSERTKELKEQQKQRNTFMM
                     NIFHDLRTPLFVLNGCMKRVSSDSGKIIQELPVIMERLDFTQHLVEDLFLMAKFEDNQ
                     VILETERVPLSDLLDKVIKACLIVSEKKEVLLEYKKYNECITWGDEYRLEQAFQNLIV
                     NAIYYTKPKSNVYIDLKSIDNMAYVSITDTGAGISEGDLDKIFDRYYRVSGTEKHQST
                     GLGLSIAHEIIKQHQGSISVKSEIGKGTIFTVQLPVVL"
     gene            5506..6195
                     /locus_tag="PKK75_05345"
     CDS             5506..6195
                     /locus_tag="PKK75_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ83057.1"
                     /translation="MTRVLLIEDDPLVAKTILYYLDNSETYEVVWAKTGGEAFANARD
                     KYDVILLDILLPDVDGIELCERLRQWHDCPIIFISCLDRSDIIVRALELGGDDFLVKP
                     FDNKVLEARIQANLRRYRKIIPSTQNELKCDGFTLDVNKHIIIKDGEEIKLSNTEFRI
                     LSFLMQNQGQYFTPKELYLKIWGEKSYGDTRTVIVHIHNIRNKIEPDSENPIFLKMEW
                     GKGYYFCKKSN"
     gene            6428..8542
                     /locus_tag="PKK75_05350"
     CDS             6428..8542
                     /locus_tag="PKK75_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ83058.1"
                     /translation="MKTKKLIAVILCCCMILSIVGCAQQTQTPPPVSEEKPEPAEEEK
                     PLINGSFEGTSTGMQGPITAKITVKDSKITAIEFTENAETANVTVVAFERIPAQIIEN
                     QSLSVDAVTGATLSSFGIIGAVTNAAKSAGLDVDSLKANKVAATPKDPQTWDTDVLVM
                     GGGGAGLTAAISAAEGGAKVILIEKGSVIGGNTMMAGAAYNAVDAEAQGAMILTKSQK
                     TTLDEYLALDPADDSLKFDVYPEWEEVLTELKADINAFYSANKGKEAGVDMPGFDSVA
                     LHMWHIYTGGLRQLNNGSWVAPNLELARTLASSSLNTFKWMGEIGLNASYGAEAKYGG
                     RPGIGTVLGAMWPRTHSFMSGAERIPHLAKVAQDKGVTIYTETRGTEIIMDGTGKVVG
                     AKAEQTDGTQITINTSKGVVLATGGYCANPAMVKQYDQYWGNDLSDRTLSTNMGTNEG
                     DGIIMAQAVGADVTGMEVAQMMPSSSPVKGTMTDGIWADAAEQIWIDGNGNRFVNEYA
                     ERDVLAKASLALDNGIFYIIYAGRGDVGNPSQLIQGTDYNERVAAMVEGGHIWYGETL
                     AELAEATKTKAAGVAPAYTEEQLRATIEKYNSYVANQKDDEFGKEVLAGAIDLDYIDA
                     TEGFGICISPRKASLHHTMGGIVIDTETHVIDTNGNIIPGLWAAGEVTGGIHAGNRLG
                     GNAIADIFTFGRIAGTNSALGK"
     gene            complement(8643..9848)
                     /locus_tag="PKK75_05355"
     CDS             complement(8643..9848)
                     /locus_tag="PKK75_05355"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNZ83059.1"
                     /translation="MNIKIPKEVENVLNILHENGFEAYVVGGCVRDSIMGKIPSDWDV
                     ATSAKPDQILLCFNNRKTIETGLKHGTVTVVINKMQIEITTYRIDGEYSDNRRPDEVL
                     FTDKIKLDLRRRDFTINALAYNKSGIIDLFGGLDDINNKFIKCVGAAGERFNEDGLRI
                     LRALRFASVLNFDIEENTSSSIFRNKALLNNISMERINIEFSKLIMGSNYRKILIEYR
                     EVIEVFLPEIQVLTEDEFKYRLNAMKVLNSLTLRLAVFLYNTDATDKILMNLKYDNKT
                     IKTVKILVENINGRINHDPVNVKRWLNKINYVNLNYLIKIKKSLFDSEFYELEETEKI
                     MNEIIRTKQCYSLETLAINGQDLIDAGVPKGKNIGIILDILLNEVIEGKLVNEKDSLL
                     NFIYVNKMI"
     gene            10124..11125
                     /locus_tag="PKK75_05360"
     CDS             10124..11125
                     /locus_tag="PKK75_05360"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNZ83060.1"
                     /translation="MDKFEKISAMINNTPMMEISLKYRGIERKVYAKAEYYNLTGSIK
                     DRVAYNILKTAYESGLIKIGDIISEASSGNTGIAFSAMGAYLRNPVHIFMPDWMSGER
                     KKLLESYGATIHLISKEEGGFKGSVESADEFGAENKAFLPHQFSNPLNAETHYKTTGL
                     EIIRQIAAFGKKPNGFAAGVGTGGTLMGVKYALQGIHPDCMAFPIEPSESPAMSSGGL
                     ASGFHRIDGIGDGMIPDIVKLKELNDIICVDDGDAINMARRLALELGIGVGISSGANL
                     IGSIKAQNILNNKDAVIVTVFPDDNKKYLSTALMKEQEEKPDYISRDVELLGFTAIR"
     gene            complement(11126..>11974)
                     /locus_tag="PKK75_05365"
     CDS             complement(11126..>11974)
                     /locus_tag="PKK75_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ83061.1"
                     /translation="GGGTVSYVSPYAYDYIDNVQLREMAGTPGILQIFKAALVMELKD
                     LAGLDKIKGKEKYYTEKVFKRLSSIENVEVLGPADVNIRLPIFSIKIKHEDKYLHPKF
                     AAKLINDLFGIQTRAGCACAAPYGHRLLDISEETSHLFRYFVKEKITAVKPGWIRFNI
                     HYIMSEKEVDFICNAVEFIAEYGYLFMNEYILDIKTGNWSHKYHNKPFSIVENFGIEE
                     SLKYIDTADTKAEGILSPNSRNQEYNKYLDEAKNHAEQLKEKVINFKSFDEKECPSWF
                     YFVNSK"
BASE COUNT         3974 a         2092 c         2220 g         3688 t
ORIGIN      
        1 aaatattgac tgcttaagtg cgttctctcc tctgactgca tagactatct atcccttagg
       61 taaagtatct gctttacaag ggatgtcccc gatttggaat ctctgacggc aggaggatta
      121 tccgaaaaaa atcctcgttg aaaatatctg gaagtaatga tatattaata agagaaaacg
      181 aggtgatttt atgtacgaaa ttcgtcaaca acaaagaaaa caaatgagag aacacaggtt
      241 tttttaccac tttatactgg taataggtat ttttgtattt gcacagggct gccagctaat
      301 gacaaaaaat ccggggtatt cggcatctgc agctatcctg ggaatcatgc tgcaaagaca
      361 cagcgtggac aaagtattta ttagaatttt caaatttgat ccccacaaaa acgcaaaagt
      421 tgcaatgata cttcttttat gtgtaattgc cgtcttcagt tattttaaaa gactaagatt
      481 catattcttc attcttctgg atttatcatc cattatctta tttgcagcaa cagcatttat
      541 atatcaaaaa ttaaaaaccc gacaggagta aaaaatgcta aaaagattta tatcgtatta
      601 caagccggta aaaaaaatat ttattgccga tatgatttgt gcctttgcag tggctgtatg
      661 cgatttgttt taccctatga ttacaagaaa catcataaat atttatgttc ccaatcagca
      721 gcttcagctt atgattacat ggcttgttgt tttaggtctc atatacattc ttaaattcgg
      781 acttaattat tacattacat attacggtca tattatgggt ataaagatgc aggctgatat
      841 gaggaaagac atatttgagc atcttcagga tctgcccttt gtatttttcg acgaaaataa
      901 aacaggaaat ctttcatcca gaattatcaa tgatttaatg gacatttcag agcttgccca
      961 tcacggaccg gaagactttt tcatatcgct ggttatgctc ataggcacat ttataatgct
     1021 gagcacaatc aatctgccgc ttgcactcat tgtattttcg attgttcccc ttttgatgtt
     1081 tgttacaatg aagcttagaa tcaagatgga agatgccttt atggaaacaa gggttacaat
     1141 aggtgaagta aatgcaacaa ttgaaaacag catatccggt ataagagtgt ccaaggcatt
     1201 cagcaataaa gaaaatgaaa tcaataagtt caacaaaaat aacagcagat ttcaggaagc
     1261 aagaagaaaa gcctacaagg taatgacaga attttatgtg ggctcttcat ttataattga
     1321 cattttaaat attgtagtcc taagtgcagg agccgtattc tttttcaagg ggagtattaa
     1381 ctttggagat tttgcagcat ttttactgta tataggtaat tttttaaatc ccataagaaa
     1441 gctggtcaac tttgttgagc agtatcaatc aggaaaatca ggatttaaaa ggtttgctga
     1501 aataatgaat aaagaaatag aaaaagatga tcccggtgca gtggatatat atgatgtaaa
     1561 gggtgacata agtttcaatt atgtaacctt tgcttatgat gataataagg aagtattgaa
     1621 ggacataaca tttgatatag aagccggaaa gacggttgcc tttgtaggcc cctccggtgg
     1681 aggcaaaaca accctttgcc atctgatacc ccgtttttac gagatagaag atggcagcat
     1741 atctattgac aacatggata tcagaaggtt tacccgcagg tcgctgcgaa aaaacatcgg
     1801 aattgttcag caggatgtat tcctgtttac aggaactatc ctcgaaaata ttcaatacgg
     1861 tagatttgat gcatccaggg aagaagtata tgaagcagct aaaaatgcca acattcatga
     1921 ttttataatg tctctgcctg aaggatatga cacctatgta ggtgaaagag gagtgaaatt
     1981 atcgggagga caaaagcaaa gaatttcaat tgccagagta ttccttaaaa atccgcccat
     2041 tttggttttg gatgaggcta cctccgctct tgacaatgct accgagcttc tgatacaaaa
     2101 agccttggat aaattgtcaa aaggcagaac tacagtggtt gtagcccaca ggttgtctac
     2161 aattaaaaat gctgatgaaa ttataatatt gactgaccag ggcatacagg aaaagggaaa
     2221 acaccaggaa cttttgaata aaaacggcat ctatgcggga ttatataatt cacagttcat
     2281 tgaagaagga gaattataat gaaagtaaca atagtcggtc attggggagg attcccgaag
     2341 gtaaaagaag caagctcggg atatttaata gagcatgatg attataagat tcttttggaa
     2401 tgcggcagcg gagttataag cctgctgcag gaaaaaacgg atataaagga ccttgatgca
     2461 gtattgataa cccattatca ctatgatcat tgctgtgaca taggaccctt gcagtatgcg
     2521 gtccagatta aaacacagct taaggaaatc agtaagcctc tccctattca tgcgccggat
     2581 ggagagtttt tcaagcttct taagtgggaa gattacacat atggagtaag ctttgatgag
     2641 aacagcagtc taactctggg accttttgaa ataagattta taaaaaatat acatcctgta
     2701 gaagcataca gtgtaagaat aaaatgcagg ggtaaaatat tttcatttac ttcggacacc
     2761 tcatattttg aagagctggc agagtttttt tccggttctg atttattgat ttgcgaatgc
     2821 agtttttatt ctgatatgga cggcacaaag gccggacacc ttaacagctc ccaggcaggg
     2881 ctgcttgcag aaaaggcaaa tgtaaagaaa ctgatactaa cccaccttcc tcatttcggc
     2941 gaattgaata atctgctaac cgaaacaatg gaatattaca agggagatgt ggtattagcg
     3001 tcatgcttga tggaaataaa tttataaaaa tcgtgaaccc ccgttttatt agaattgaac
     3061 ccaattcatg attaataacg ataaatataa tatgattgag gaagtgaagc tatgatagat
     3121 gtgaaaggca gacgaaaatg tgagaactgc ggcaaaagat ataattatac atacacaaaa
     3181 ggtgaagaag cttcaaaaaa agtaaaattt aagggcaaag atgaaaatct aagcactgta
     3241 acaaatgtta aggtgctttc aatacatact tatgaagtaa ccgccagatg cccggaatgc
     3301 gggttcgaag aaatatttat ttacaacgat taagatgcag gaattttttc ccggctctga
     3361 cttgtttata tataaaaagc tgatacttac ccgctttcct catttcagcg aattgggtaa
     3421 tctgcttact aagcaaagaa cattacaagg aagagatggc aatcagcatc atatttgatg
     3481 gaaattactc tataaaaatc tatggaggta agaccaggat attgatttat gataatgtta
     3541 ttttataata ctacaggaag ctgtaccgta aaaattgttc ctttcccgat ttcgcttttt
     3601 acagagatgc ttccctgatg ttgtttgata atttcatgcg ctattgatag tcccaatcct
     3661 gttgattgat gcttttcagt cccgctcacc cgataatacc tgtcaaatat tttatctaaa
     3721 tccccttcag atattcctgc acctgtatca gttatcgaca cataagccat attatcaata
     3781 gactttaaat ctatatagac attactctta ggttttgtat aataaatggc attcacaatc
     3841 aggttttgaa atgcctgctc caggcgatat tcatcacccc atgttataca ttcattatac
     3901 tttttatatt ccaataaaac ttcctttttt tcacttacta ttaaacatgc ttttattacc
     3961 ttgtctaaaa gatcgctcag aggtacacgt tctgtttcaa gaattacttg attatcctca
     4021 aacttagcca tcaagaataa atcttcaact aaatgctgag taaaatccaa cctttccatt
     4081 attacaggca gctcctgtat aattttaccg gaatctgagc tgactctctt catgcacccg
     4141 tttaaaacaa agagcggtgt acgcaaatcg tgaaatatat tcatcataaa agtatttctt
     4201 tgtttctgct gttcttttaa ctcttttgtt ctctctgata ctttgacttc caatacttga
     4261 tttgattgct ccagctgtgc agacaaagcc tctgattctg aaaacttttc tgcaaaaaga
     4321 cgattaatca ataacaagca gcatattgca aatggaatat taaatgattt tgaatacctc
     4381 ataactctca gccaaaagct cacattcata gtattaagag aagaatatgc cacaaccaat
     4441 ctcattccaa ggctaatagt atgtcctaaa agaagcagcc atgcaccctt ccttttgttt
     4501 gcacacgcat atataagagc tccgatagaa aaaaagaata aataataata atcctttaag
     4561 atgtttggca gcagtgtttc aaagaatgcc catgcaatca gcatccccgc cactccaccg
     4621 ccgcttaaca acttgtcaaa aggtccggga agcttagtct taaatatttt aaagcaaata
     4681 ataatatcaa gaagcgcaca ccatccatat gcatgtgata ccaactcttt tataatgacc
     4741 gaatttgtat ccatatgaag tgagccgata ctccatagtg taagagctcc tgtatatatt
     4801 gaaaaccata ttaaatattt ttcactgggt ttatgataat aaagagatgc tccgtataat
     4861 gacattaata aagtcatacc gatattaaat gcaaatatca tataataagc ggaaataccg
     4921 ctttgggctc catttaccgt tgttaaataa acattttgat tatatggatt acttttgctg
     4981 ttaaattcta atgtcataga atttgtctgc acatcaaaca accttgaatc aatggctgcg
     5041 ctgtgaaatc ttgttgaatt gtaatttgat gaatccacaa ggatatctcc gttaactttt
     5101 atatcacctg taataaaacc atatataagt gctaattgga catttgtatc ttctaccaca
     5161 atatcaaaat aatattttcc tgtatttaaa tcttccctgt atccaataag tgaatgaatt
     5221 tcattttcat ctgataaaac aaaaacttgt ccgctttcat acaagctaaa actattattg
     5281 attgtgataa aaactaaaaa agcaattgct aaagcaatta taaaagctat tattggtttc
     5341 ccgtaatcct ttgtcatttt gcacctgttt ttttattaaa tgttatacta ttattataat
     5401 ccatgtcaag cgatatttaa atggatgttg caattatgag ctttttaaga tatgataatt
     5461 tataatcaat ataaaaagca tcctgttcaa aatgaggtga actagttgac acgagtatta
     5521 ttaatagaag acgatccatt agtggcaaag acaattctat attatttgga taattctgaa
     5581 acatatgaag ttgtttgggc gaaaacagga ggagaggctt ttgcaaatgc aagagataaa
     5641 tatgatgtta tactgcttga tatactattg ccggatgtag atggaattga gctttgcgaa
     5701 agactaaggc aatggcacga ttgtccaata atatttatat catgccttga cagaagcgat
     5761 attattgttc gtgcattaga actcggagga gatgattttc ttgtcaagcc ttttgataat
     5821 aaagttcttg aggcacgaat acaagctaat ttaagacgtt accgcaaaat aattccctca
     5881 acacaaaatg agctaaagtg tgatggtttt actttagatg ttaataagca tattattatt
     5941 aaagatgggg aagagataaa gctatccaat acagaattta gaattctttc ttttcttatg
     6001 caaaaccagg ggcagtattt tacgccaaag gaactgtatt taaaaatctg gggtgaaaag
     6061 agttacggag atacccgtac tgtaattgta catatacata acatacgtaa caaaattgag
     6121 cctgattctg aaaacccgat ttttctaaag atggaatggg gtaaaggata ttatttttgc
     6181 aaaaagtcaa attaagatac aattaatatt tttatataaa caattaagat ttcttaattt
     6241 cccttgatac ttatttaact aatttcaagg gattttttga tttttgaata caaataaaga
     6301 atacttttag ctgtatttcg acataaattg ccattattat aattaaatta ttaataactc
     6361 cttaactgct ttatcaaaaa aatcatgtta taatttaggc acatttatta gagaagggga
     6421 gaaagttatg aaaacgaaaa aattaattgc tgtgatttta tgttgttgta tgattctttc
     6481 aatagttgga tgtgcacagc aaactcaaac accaccaccg gtaagcgaag aaaaacctga
     6541 gccggcagaa gaagaaaaac cacttattaa tggcagcttt gaaggaacct ctacaggtat
     6601 gcaaggacca ataactgcta aaatcactgt aaaagacagt aaaataactg ctatagaatt
     6661 tacagaaaat gcagaaactg ctaatgttac agttgtagca tttgaacgca ttcctgcaca
     6721 aataattgaa aatcaaagtt tatctgtaga tgcagttacc ggtgctacct tatcaagctt
     6781 cggtataatc ggtgcagtta ctaatgcagc aaaatccgcc ggcttggatg ttgattcgtt
     6841 aaaggcaaat aaagtagctg ctacaccaaa ggatcctcag acatgggata cagatgtttt
     6901 ggttatgggc ggcggaggag ctggactaac tgcagcaata agtgctgctg aaggaggggc
     6961 caaagttatt ttaatagaaa aaggctctgt aataggcggt aatacaatga tggcaggagc
     7021 agcttacaat gcagttgatg cagaagcaca aggtgctatg attcttacta aatctcaaaa
     7081 aacaacatta gacgaatatt tagcattgga ccctgctgat gatagtctaa aatttgatgt
     7141 ttatcctgag tgggaagaag tattaacaga attaaaagca gatattaatg ctttctattc
     7201 cgcaaacaaa ggaaaagaag ccggagttga tatgccgggc tttgactctg tggcattgca
     7261 tatgtggcat atttacacag ggggattgcg tcaattaaat aacggatcat gggttgcacc
     7321 taatttagag ctggcaagaa cacttgcatc aagttcactt aacactttta aatggatggg
     7381 cgaaattgga cttaacgctt catacggtgc tgaggctaaa tacggcggac gacccggaat
     7441 aggaactgta cttggtgcta tgtggccgag aacacacagc tttatgtcag gagcagaacg
     7501 tatacctcac ttagcaaaag ttgcacagga taaaggtgta actatttata cagaaacaag
     7561 gggtacagaa ataataatgg atggtacagg taaagttgta ggtgcaaaag ctgagcagac
     7621 agatggaacg caaatcacta ttaacacatc aaaaggcgtt gttttagcta ccggaggata
     7681 ctgtgcaaat ccagcaatgg tgaagcaata tgatcaatat tggggtaatg acttatcaga
     7741 cagaacatta tcaactaata tgggaaccaa cgaaggtgat ggaattatta tggctcaagc
     7801 agtcggtgct gatgtaaccg gaatggaagt tgctcaaatg atgccttcat catctcctgt
     7861 taaaggaact atgacagacg gtatttgggc agatgctgca gaacaaattt ggattgacgg
     7921 aaacggcaac cgctttgtta atgaatatgc agagcgtgac gttttagcca aagcatcatt
     7981 agcacttgat aatggtattt tctacattat ctacgcaggc agaggagatg ttggtaatcc
     8041 ttcacagctt atacagggta ctgactataa tgaacgagtt gctgctatgg ttgaaggggg
     8101 acacatatgg tatggagaaa ctcttgcaga attagctgaa gctactaaaa caaaagctgc
     8161 aggtgttgca ccggcatata ctgaagagca gttacgtgca actatagaaa aatacaactc
     8221 ttatgtagct aaccaaaaag acgatgaatt tggaaaagaa gtattggcag gagctataga
     8281 cttggattat atagacgcta ccgaaggctt cggaatatgc ataagcccga gaaaagcttc
     8341 gctgcatcat acgatgggtg gtattgttat agatactgaa actcatgtta tcgatacaaa
     8401 cggaaatata atcccgggtt tatgggcagc cggagaagtt acaggaggta ttcatgccgg
     8461 taacagatta ggtggaaatg caatagctga catattcacc tttggacgta ttgcaggtac
     8521 taattctgca cttggcaaat aatagttaag tttagtcaaa gatagattta aaataaaaaa
     8581 gagagctttt agaaaactac ttctttaaaa gctcttttta tttcctttaa gtcccatccc
     8641 gcctatatca tcttgtttac ataaataaaa ttcagtaaac tatccttttc attaaccagc
     8701 ttgccttcaa taacctcatt caaaagtata tctaaaatta tgcctatatt cttacccttg
     8761 ggtacgcctg catctataag gtcctgcccg ttgattgcca gtgtttccaa gctgtagcac
     8821 tgtttggtcc gaataatttc gttcatgatt ttttcggttt cttccagctc gtagaattct
     8881 gaatcaaata atgacttctt gattttaatc aaataattca gatttacata atttatttta
     8941 tttaaccacc ttttaacatt cacggggtca tggtttattc tcccgtttat attttcaaca
     9001 agtatcttaa ctgtttttat agtcttattg tcatacttta aattcatcaa aatcttatca
     9061 gttgcatcag tattataaag aaatacagcc agccttaaag ttaaagaatt taataccttc
     9121 attgcattta accgatactt aaactcatct tcagttaaaa cctggatttc aggcaggaag
     9181 acctcaataa cttctctgta ttcaatcaga atttttctgt aattgcttcc cataatcagc
     9241 ttgctaaatt caatgttaat ccgctccata gaaatattat taaggagagc tttgtttctg
     9301 aaaatgctgc ttgaagtatt ttcttctata tcaaaattta aaacagaagc aaaacgcagt
     9361 gcccttaaaa ttcttaaacc gtcctcgttg aacctttcac cggcagctcc aacgcatttt
     9421 ataaatttgt tatttatatc atcaaggccg ccgaacaaat caattattcc acttttgtta
     9481 taagcaaggg cattgattgt aaaatccctt cgtctcaaat ccaatttaat tttatccgta
     9541 aataaaacct cgtcgggacg cctgttatcg ctgtactccc catcaattct ataggttgta
     9601 atctcaatct gcattttatt tatgacaaca gtaacagttc cgtgttttaa acctgtttct
     9661 atggttttgc ggttattaaa gcacaataat atttgatccg gctttgcaga tgtggcaaca
     9721 tcccagtcag aaggaatttt tcccataatg ctgtctctta cacagccccc tactacatat
     9781 gcttcaaatc cgttttcatg caaaatattt aatacatttt caacttcctt tggaatctta
     9841 atattcattg gaatccttct ttttgccttt ttatataatt ataaccaatt tttcagatat
     9901 aacaaggaga aactggcaaa gcagcagaag aagtaataaa gaaatgatag aataacatcc
     9961 ggcattctta ggagttcttg cattttacag aggtacttaa cagaaacacg agtttgttga
    10021 caaatgtagc aacaatccct ttcaccggtc cacatgagtc actttttgat tttaatcata
    10081 taataagcta aaatgtccat aataatagaa aggatatgat ttaatggata agtttgaaaa
    10141 aatatctgca atgataaata atacccccat gatggaaatt tcattaaaat atcgcggaat
    10201 tgagcgtaaa gtgtacgcga aggcagaata ctacaattta acaggcagca tcaaagacag
    10261 ggttgcttat aatatattga aaactgctta tgaatcagga ttaatcaaaa taggggatat
    10321 aatttcagag gcatccagcg gcaacacagg cattgcattt tctgcaatgg gggcatattt
    10381 aagaaacccc gttcatattt tcatgccgga ttggatgagc ggtgaaagaa aaaaactgct
    10441 ggaaagctat ggcgcaacaa tccacttaat ttcaaaggaa gaaggtggat tcaaagggtc
    10501 ggtggaatca gccgatgaat tcggagctga aaacaaggcc tttttacctc atcaattttc
    10561 aaatccttta aatgcagaaa cccattacaa gacaaccggc ttggaaatta tacggcaaat
    10621 cgctgcattt ggcaagaaac cgaatggttt tgcagcagga gtaggcacag ggggaactct
    10681 tatgggggtg aaatatgctc ttcaaggaat tcatcctgat tgtatggcat ttcctattga
    10741 accctcagag tctcctgcta tgtcttcagg tggtcttgct tcaggttttc acagaattga
    10801 tggaattggg gacgggatga ttcccgatat tgtaaaatta aaggagttaa atgatataat
    10861 atgcgtggat gacggtgatg ctatcaatat ggcgagaagg ctggctcttg agctgggaat
    10921 cggtgttggt atttcatcgg gtgctaattt aataggttcc ataaaggcac agaatattct
    10981 caacaacaaa gacgcagtta ttgtaacggt tttccctgat gacaataaaa agtatctgtc
    11041 tacagccctt atgaaagaac aagaagaaaa gcctgattat atatccaggg atgtggagct
    11101 gcttggattt acagcaataa ggtaatcatt tagagtttac gaaataaaac catgatgggc
    11161 attccttttc atcaaaggat ttgaaattta taaccttttc tttcaattgt tcagcatggt
    11221 tttttgcttc atccaaatat ttattatact cttgatttcg gctgtttggg gataatatcc
    11281 cctcagcctt tgtatcggcg gtgtcaatgt attttaaact ttcctctatg ccgaaatttt
    11341 ccacaatgct aaatggctta ttatggtact tatgagacca attgccggtt tttatatcta
    11401 aaatatattc attcataaac aaatacccat attcggcaat aaactccacg gcattgcata
    11461 tgaaatcaac ttccttttca ctcattatgt aatggatgtt aaaccttatc cagcctggtt
    11521 ttacagctgt gattttttct tttacaaaat accgaaacag atgagaagtt tcttcactta
    11581 tatccagtaa cctgtgaccg tagggagccg cacaggcgca gcccgctctt gtttgtatgc
    11641 caaagaggtc atttatcagt ttggcagcaa atttaggatg aagatattta tcctcatgtt
    11701 ttatttttat tgaaaatata ggaagcctaa tatttacatc agcaggacct aaaacctcga
    11761 cattttcaat tgagcttagt cttttaaaaa ctttttctgt ataatatttt tcttttccct
    11821 ttattttgtc taaccctgcc aaatccttta attccattac caatgctgct ttaaatattt
    11881 gaaggatccc gggagtaccc gccatttctc tcaactgtac attatctata taatcatagg
    11941 catagggact cacatagctt actgtcccgc cgcc
//
LOCUS       DAOPUT010000046        11939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_5467_length_11939_cov_17.911225, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000046 DAOPUT010000000
VERSION     DAOPUT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11939
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5467_length_11939_cov_17.911225"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1684
                     /locus_tag="PKK75_05370"
     CDS             206..1684
                     /locus_tag="PKK75_05370"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNZ83062.1"
                     /translation="MNIKSLLKEMDYLSFTGSDDTEITGITNDSRKVGKNDIFVAIKG
                     FTNDGHKFIAKALENGAAAVICENIPENVRGNGPFILVKSPRESMAIAADIIYGRPSK
                     KMNIIGVTGTNGKTSTTYLLNGIYDYLGEKSGIIGTMGVLIDGKKIKVDNTTPESFDI
                     QHYLAMMLEKGITYCSMEVSSHALELNRIDEVHIDVGIFTNVTRDHLDFHETMENYYQ
                     AKKKLFHLTKINNIINVDDPYGHRLYNELIAEGVKAISIGIENDADIRASNLKTTMKG
                     TSFDLNVQGIKKKAHVNTPGTFSVLNSLGALGAAYALGVNIDDIIEALEEIRGVTGRF
                     EILPNKLDCTIILDFAHTPDGLQKVMDTINEFAQGRKIVMFGAGGERDLSRRGPMGEI
                     AGKYCDLSILTSDNPRFEDPYEICLEIAEGVKKHNGQYKIIIERDQAIYYAIDNSQKN
                     DVILLAGKSTEPYQDMGIEKVPYDETMLAKRAIKDVEKKRGV"
     gene            1877..2851
                     /gene="mraY"
                     /locus_tag="PKK75_05375"
     CDS             1877..2851
                     /gene="mraY"
                     /locus_tag="PKK75_05375"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNZ83063.1"
                     /translation="MQGNEQIIRVIIVSFLIALFLGPVVIPVLKRLKVGQSIRKDGPK
                     SHLVKSGTPTMGGIIIILAILISILTSGFFKAEIWAVIFFILGFGLIGFIDDYIKVVM
                     RQNEGLKPMQKIIGQLIFSVILAWYGSKYSAMGTTLMIPFVNIHIDLGVLYIPFITFF
                     AVGMVNAVNLTDGLDGLNTGVTMIVMAAFSLIANSLKDANQIYEGISLLSAAVSGACL
                     GFLRHNANPAKVFMGDTGSMALGGAVAAVAVISNTVLFIPIIGGIYFAEALSVMIQVL
                     HYKRTKRRVFKMAPLHHHYEMSGWKETKVVAVFWIASLILAFIGIYSI"
     gene            2894..4258
                     /gene="murD"
                     /locus_tag="PKK75_05380"
     CDS             2894..4258
                     /gene="murD"
                     /locus_tag="PKK75_05380"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNZ83064.1"
                     /translation="MNVLVVGLGVSGIACIKGLSRTGEKIYAFDESPKENLKDRLKEV
                     EGIEAEYYFGNEELKKINMKNLDYAIKSPGIKYEVPVIEMLLKNNVEVISDIEAAYRA
                     TDATIVSITGTNGKTTTTTLTGEMASESGMLFKVTGNIGFGMYNDALNAKKGDVLVAE
                     VSSFQLAGTSRYKPHISLITNITPDHLDYHHTVENYIEAKFKNVINQDENDYAILNYE
                     DATIRNFSDKIKAKKIFFSSGRILEDGIFAENDRLYYKNKNNKQFIISTKEIFIPGKH
                     NLENAMAAAGAAIALGIDIEIIAGVLKEFKGVEHRLEFTGEYNKVKFYNDSKGTNPDA
                     SIKAIQGIEKPIVLIAGGYDKKSSYDEFIKAFDDKVKALILLGQTAKDIERCALKYGF
                     ENIYLVETMDEAVKKSFELAEEGDNVVLSPACASWGMYPNYEVRGRDFKERVSYYGGN
                     SKKS"
     gene            4233..5354
                     /gene="ftsW"
                     /locus_tag="PKK75_05385"
     CDS             4233..5354
                     /gene="ftsW"
                     /locus_tag="PKK75_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNZ83065.1"
                     /translation="MEETVKKAKTRSIDWVLVFIIVLLVLFGFLMVYSSSYPDGYYKY
                     GGDPFYFLRKQVIAAGAGLAGMIFFANLPYVIYKKFSKLILFACIGLAMLTIVIGIAS
                     NGAQRWITIGGISLQPSEIIKIGAVLYMADYFSRRKNELTSFTKGFLPSLFYIGLFCG
                     LIAVQDDLSTAVILAGTLMVMFFCAGAKISHLLLLFILAVGGVIGLIATQTYRISRII
                     VFFDPFKYPLDEGYQIINSLYALGTGGLFGMGIGQSRQKFFHLPEAYNDFIFSIIGEE
                     LGFAGCLLLMLGFVVFAWRGLRISMNTEDFFGSQTALGLTVIVLAQFLIHVAVATSSM
                     PPTGRALPFISAGGSSIMFLLCSMGILLNISKYSNTYRN"
     assembly_gap    5364..5463
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5505..6620
                     /gene="murG"
                     /locus_tag="PKK75_05390"
     CDS             5505..6620
                     /gene="murG"
                     /locus_tag="PKK75_05390"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNZ83066.1"
                     /translation="MKVLITGGGTGGHINPALAIAQKVKSENPSDAVLYVGSRNSLES
                     ELIPKEGFEFKAVTAKYLKRKITFDNIKTLFASAKGLMESFKIINEFKPDIVVGTGGY
                     VCGPVVLAAYLKKIPTMILEQNVYPGITNKMLSRISDRIGISFEEARQYFPQSAREKL
                     IMTGNPVRKDIITTDRRKSRTRYNLKPEDVFIYSFGGSGGQISLNEAIIGVIKKYNGS
                     SNVKILHVTGRKHYDSFMEELNKSIKPEKNIEIKDYMYEAPLALSASDIVIGSAGAIT
                     IAEITAIGVPSILIPKTYTAENHQEYNARALEKEGAALVILEKDLSDKTLLKSIEEII
                     ENKGRLKTMSLNSKRLGSSDVENKIYTIMTDLINSRK"
     gene            6680..7939
                     /gene="murA"
                     /locus_tag="PKK75_05395"
     CDS             6680..7939
                     /gene="murA"
                     /locus_tag="PKK75_05395"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNZ83067.1"
                     /translation="MGSYIVEGRNSLEGIVDIGGSKNAALPIIASTVITGREYILENI
                     PDIEDVKDMLEILNIMGCSVQLENGIALIDTRGLNTHVVPEKLVRKIRSSIILMGAVL
                     QRTGKVEVAYPGGCEIGLRPIDLHLKGLRKLGVEISEKNGLLSGIVKEPATCDIHLDY
                     PSVGATENIMLFAVGNPGKTTIYNPAREPEIVDLQNFLNTAGFCIDGAGTGLITIEGK
                     KIENTDTVEYKIISDRIEAGTYLSAAASMKSKIYIRNIEKSHFKSITSIFEDAGCEIR
                     ERHGTIIIANNKRLKAIEKVTTQPYPGFPTDMQAQVMASMTVADGISIIEETVFDSRF
                     KHVPELQKMGACITTIGRVAVVDGIEKLSSAEVEAKDLRGGAALVIAAMGAEGTSKIN
                     NIHHIKRGYEKFLYKLQNLGVIITETY"
     gene            8035..8832
                     /locus_tag="PKK75_05400"
     CDS             8035..8832
                     /locus_tag="PKK75_05400"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNZ83068.1"
                     /translation="MKECPRFMENNNKKQRKKKKKRNKRLLISTAAFITAAVVVVFCI
                     YYVYYETPYFDLIGVDMEGNLTYSDEYIMEKSGIETGKKIFDIDRRQVKENLENEVYV
                     ENIRVVYTLPNRIFLDIVERVDKYQVFINNEYIIIDKNGIVLRTSTDKSDLLTIESYA
                     EVVYNIGDSIQFAGIENIEDVFDAIEYLNNQYGTDAIKSCSVDQDNSFLFKTRYGTGV
                     RLDLSEDINYQIVFAMKIINERLNKNLTVSSGLIDFTKGDSPVYIED"
     gene            8845..9558
                     /locus_tag="PKK75_05405"
     CDS             8845..9558
                     /locus_tag="PKK75_05405"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HNZ83069.1"
                     /translation="MNKIWQKTMMFAISLVLGIVLITQMRTKTGILGMVNLEEKSLEL
                     NEKLAQLNLEKTRLRDELEALKEKNREYEEIYADAEEELIGLSEELEDQKIISGYYDV
                     KGPGTIITIDADPNSYMSLASSHQYILALISYLNNAGVEAISINGQRYTNYTEIVPVL
                     EHINVNGVAMVLPLEIKAIGNSATINSSLNFVGGIVTQFNGIGYIIETEDSDEIFIQR
                     YEGEKQFKYAVPIAREEEI"
     gene            9562..10293
                     /locus_tag="PKK75_05410"
     CDS             9562..10293
                     /locus_tag="PKK75_05410"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HNZ83070.1"
                     /translation="MVIIKKSFNIIIFILVFLTAAALVNGADAGINEDSIYDLKKLKE
                     YERSKKEMELLVENMRKAVNSEKDKIAYYEKREYFDDIIEINSQIEEIKLYNGFTDVR
                     GPGIMLRISDNINEDENLDIMERIVHDVDIVVLLNDLKAAGAEAIEINGKRITNMSEV
                     ICAGPLIRVNNEVIPAPFVIIAIGDMDELYDAVTMEDTYAYELMDVYGMEVVAIKGYN
                     LTVRKFRGINYEPQFAEIIKEEVEE"
     gene            10290..10634
                     /locus_tag="PKK75_05415"
     CDS             10290..10634
                     /locus_tag="PKK75_05415"
                     /inference="COORDINATES: protein motif:HMM:NF018632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HNZ83071.1"
                     /translation="MIYVAAGLILGIVAGLNLNLVYNPNYIVYISLAILAVLNTIINM
                     LHDSKTTELSMGKSIAYLLTDLLFALFLGFVGEQSGLPIYLAAVFAFGNNIYKKLRLL
                     VDIYLEKFSKNK"
     gene            10720..11772
                     /gene="ftsZ"
                     /locus_tag="PKK75_05420"
     CDS             10720..11772
                     /gene="ftsZ"
                     /locus_tag="PKK75_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNZ83072.1"
                     /translation="MFQFEEPSEKLANIKVIGVGGGGNNAVNRMIDAGVRGVNFISVN
                     TDKQHLSTSKAETKFQIGQKLTNGLGAGSKPEIGEKAAEENKQDIAELVEGADMVFIT
                     AGMGGGTGTGAAPIIADIAKSMGILTVAVVTKPFMFEGRCRMKNAEMGLKRLKENIDT
                     LVVIPNDKLLEITDKKTTMLEAFAMADDVLRQGIQGISDLIAIPAIMNLDFADVETIM
                     AGQGLAHMGIGQATGENRALNAVKQAIQSPLLETSINGARGVLVNITGGISMGIHEVN
                     EAAKLIEEAADPEANLIYGANIDEALGDTVKITVIATGFGESKAKKPEGEAAESNEKK
                     PESPFDFTIPSFLNKK"
BASE COUNT         4180 a         1910 c         2599 g         3150 t
ORIGIN      
        1 cgctcaaaca gtgcaagctg cttaacggat ttttaacctt cattcgctaa gaattatagt
       61 cgtgtcctgc aaatgttcgc ttaatatttt tcttattttt cgagatgctt tgaaagctta
      121 tgattagaaa gacatattat tgtaaaaggt cattatttat gataaaattg cacatactat
      181 catacattta aatagaggta tacttatgaa tataaaaagc ttacttaaag agatggatta
      241 tttaagcttc acaggcagtg acgatactga aataacaggc attacaaacg attcacgaaa
      301 agttggcaaa aatgatattt ttgtggcaat taagggtttt acaaacgacg gtcataaatt
      361 tatagcaaag gctttggaaa atggagcagc tgccgttatc tgcgaaaata ttccagagaa
      421 tgtcagaggc aatggccctt ttatccttgt taaatcaccc agagaatcaa tggctatagc
      481 agctgacatc atttacggac gaccttcaaa aaaaatgaat attatcggag tcacgggaac
      541 taacggcaaa acctccacta catatttatt aaatggaatc tatgattatt taggcgaaaa
      601 atcaggaata ataggaacga tgggtgttct tattgacggc aaaaaaatca aggttgacaa
      661 taccacgcct gaatcctttg acatacagca ctatttggcc atgatgcttg aaaaaggaat
      721 cacctactgc agtatggaag tttcatccca tgcacttgaa cttaacagaa ttgacgaggt
      781 tcacatagat gtgggaattt ttacaaatgt tacaagagat catctggatt ttcatgaaac
      841 aatggaaaac tattatcagg caaagaaaaa actgtttcat ttgacaaaaa taaacaacat
      901 tataaatgtg gatgaccctt atggacacag gctatacaat gaactaattg ctgaaggagt
      961 caaggcaata tccataggca ttgaaaacga tgcggatatc agagcttcaa acctgaaaac
     1021 cactatgaag ggaacctcct ttgacctcaa tgtacagggg ataaagaaaa aagcccacgt
     1081 aaatactcct ggaacattca gtgttctcaa ctcacttggt gcactgggtg cagcatatgc
     1141 cctgggggta aatattgatg acattatcga agcactggaa gaaataaggg gtgttacagg
     1201 aagatttgaa atacttccca ataaattgga ttgcaccata attcttgatt ttgcccatac
     1261 gcctgacggc cttcagaagg tgatggatac cataaacgag tttgcacagg ggagaaaaat
     1321 agttatgttt ggtgcaggtg gagaaagaga tttgtcaaga cgtggtccca tgggcgaaat
     1381 tgcaggcaaa tactgtgatt taagcatact tacatctgat aacccaagat ttgaggaccc
     1441 ctacgaaata tgtttggaaa tagccgaggg tgttaaaaaa cacaatggcc aatataaaat
     1501 aattattgag cgggatcaag ccatatatta tgctattgac aacagccaaa agaacgatgt
     1561 aatattgttg gcgggaaaat ccacagaacc ttatcaggat atgggaattg aaaaagtacc
     1621 ttatgatgaa acaatgctcg caaaaagagc aataaaagat gtggagaaaa aaagaggggt
     1681 ctaaggtata gggacacacc ttcaaaatag ggtgactctc tgcaagatag cttttaaagg
     1741 aatgtccctg aatttattgg gacacacctc tagtacaggg agagtcttgc gggcacaggc
     1801 ggatgaatca cccttcattg atagcaataa agaaaatgtt caggatgaca agataaccac
     1861 ttaattagga gaaattatgc agggcaacga acaaattata agagtaataa ttgtatcctt
     1921 tttaatagca ctgtttttag gaccagttgt aatcccggtc ttaaaacgcc ttaaagtagg
     1981 acaaagtatc cggaaagacg gacctaaaag tcacctggtc aaatcgggaa cccctacaat
     2041 gggtggcatt ataatcatac tggcaatttt aatttcgatt ctgacaagcg gattttttaa
     2101 agcggagata tgggcggtaa tattcttcat tttgggattt ggattaattg gatttataga
     2161 cgactatata aaggtggtaa tgaggcaaaa tgaaggatta aagcccatgc aaaaaattat
     2221 aggccagctt atcttttcag tcatccttgc ctggtacggc tcaaaataca gcgccatggg
     2281 aactacctta atgataccct ttgtaaatat acatattgat ttaggagtat tgtatatacc
     2341 ttttataacc ttttttgcag taggaatggt aaatgccgtg aaccttactg acggcctgga
     2401 cggtcttaac acaggggtta caatgatagt aatggcagct ttcagtttaa ttgcaaacag
     2461 cttaaaggat gcaaaccaga tatatgaagg aatatcattg ttaagtgctg cagtttccgg
     2521 tgcctgcctg ggtttcttaa ggcataatgc aaatccggca aaagtcttta tgggggatac
     2581 agggtccatg gctttgggag gtgctgtcgc agctgttgct gttatatcaa acacagtatt
     2641 attcatacct ataatcggcg gcatatactt tgccgaagca ctttcagtaa tgattcaggt
     2701 tcttcattat aagaggacaa aaagaagagt atttaaaatg gcgcctctcc accatcatta
     2761 tgaaatgagc ggctggaaag aaacaaaggt tgtggctgtg ttttggattg cctctttgat
     2821 attggcattt ataggtattt attcaatata aacaacataa tataataatg gacattttat
     2881 gaggacggta aaaatgaatg tactcgttgt aggattgggt gtttcaggaa ttgcatgcat
     2941 aaagggatta agcagaacag gtgaaaagat ttatgcattt gatgaatcac caaaagaaaa
     3001 tttgaaagac agattaaaag aggtagaagg cattgaagct gaatactact tcggaaatga
     3061 agaactcaag aaaatcaata tgaaaaattt ggattatgcc attaaaagtc caggcattaa
     3121 atatgaagtc ccggtaattg aaatgctttt aaaaaacaac gtcgaagtaa tcagcgacat
     3181 agaagcggct tacagggcaa cagatgcaac tattgtttcc ataaccggaa caaacggcaa
     3241 aacaacaacc acaaccctga caggagaaat ggcaagcgaa agcgggatgc tcttcaaggt
     3301 caccggtaac attggttttg ggatgtataa cgatgcattg aatgcaaaga agggagatgt
     3361 acttgtagcg gaagtaagca gttttcagct ggcaggcaca tcaaggtaca aacctcatat
     3421 cagccttata acaaacatca ctcccgacca tctggattat catcacacag tagaaaatta
     3481 tatagaagcg aagtttaaaa atgtaataaa ccaggatgaa aatgattatg caatactaaa
     3541 ttatgaagac gcaacaataa gaaatttttc agataaaatt aaggcaaaaa aaatattctt
     3601 cagctccggg agaattttgg aagatggaat atttgcagaa aacgaccggt tatattataa
     3661 aaacaaaaat aataaacaat ttataatcag tacaaaggaa attttcatac cggggaagca
     3721 caatcttgaa aatgcaatgg ctgcagcagg tgccgccata gctttgggaa tagatattga
     3781 aataatagct ggggttttaa aagaatttaa aggtgtcgag catcgtctgg aatttaccgg
     3841 cgaatacaat aaagtcaaat tttacaatga ttcaaaagga acaaatcccg atgcctccat
     3901 taaagcaata caaggaatcg aaaagccgat tgtattgata gcaggcggct atgacaagaa
     3961 gtcatcctac gatgagttta tcaaagcatt tgatgataag gtaaaggcac ttattcttct
     4021 ggggcagact gccaaagata tagaaaggtg cgctttgaaa tatggttttg aaaatattta
     4081 cctggtggaa acaatggatg aagctgtaaa aaagagcttt gagctggctg aagaagggga
     4141 taatgttgtt ttatctcccg catgtgccag ctggggaatg taccctaatt atgaagtcag
     4201 aggaagagat tttaaagaaa gggtaagtta ttatggagga aacagtaaaa aaagctaaaa
     4261 ccaggtcaat tgactgggtc ttggtcttta taatagtgct attggtgtta tttggcttct
     4321 taatggttta cagctcctct tatcccgacg gatattacaa atacggggga gatccctttt
     4381 attttttaag aaagcaggtt attgcagcag gggcaggtct tgcaggaatg attttttttg
     4441 caaaccttcc atacgtgatc tacaaaaaat tcagcaagct catactgttt gcatgcatag
     4501 gcctggcgat gcttaccata gtaataggga ttgcatcaaa cggcgcccag aggtggataa
     4561 ctattggggg catatcgctg cagccttcgg aaataatcaa aatcggcgct gttctttaca
     4621 tggctgatta tttcagcaga aggaaaaacg aactgacatc ctttaccaaa ggatttttgc
     4681 cttcattatt ctacattggg cttttctgcg gtttaatagc tgtacaggat gatttaagca
     4741 ctgcagtaat tcttgcggga actcttatgg tcatgttctt ttgtgcaggt gcaaagataa
     4801 gccatctact gttgctgttt atactggcag ttggcggggt aatcggattg attgcaactc
     4861 agacctatcg aatatcaaga ataattgttt ttttcgaccc atttaaatat cccctggatg
     4921 aaggttatca aataataaat tcactgtatg cattgggcac aggaggcctt ttcggcatgg
     4981 gaatagggca gagcagacaa aagtttttcc atttgcctga agcctacaat gactttatat
     5041 tttcaataat aggagaagaa ctgggctttg cgggctgttt gttattaatg ctcgggtttg
     5101 tggtatttgc atggagagga ctgagaattt ccatgaacac ggaggacttt ttcggaagcc
     5161 agactgctct tggcttgact gtaatagtac tggcacagtt tttaatacac gtagcggttg
     5221 caacatcatc aatgcctcca acaggaaggg ctctgccctt tatcagcgcg ggaggttcat
     5281 cgataatgtt tctattgtgc tccatgggga tactgctaaa tatatcgaag tattcgaata
     5341 cttacaggaa ttaaagatgc cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnatgccct gaccattatt atttattaaa aggaatggta ttatatgaag gtactaatca
     5521 ccggcggagg aaccggaggc cacataaatc cggcgttggc aatagcacag aaggttaaaa
     5581 gtgaaaaccc ttctgatgct gttttgtatg tgggaagcag aaacagcctg gaaagcgagc
     5641 ttattccaaa ggaaggcttt gaatttaaag ctgttacagc caaatatcta aaaaggaaaa
     5701 ttacctttga caacattaag acattgtttg catcagcaaa gggtctgatg gagtctttta
     5761 aaataataaa tgagtttaaa ccggatatag tggtaggcac gggaggatat gtatgcggtc
     5821 ccgttgtttt ggctgcatac ttaaagaaaa ttcccaccat gatattggaa caaaatgttt
     5881 atcccggaat aaccaacaag atgctgagca gaatttccga caggatagga ataagctttg
     5941 aagaagcaag gcagtatttc ccccaaagtg cccgggagaa gcttattatg acggggaatc
     6001 ccgtaagaaa ggacataata acaacagaca gacgtaaatc aaggaccaga tacaacttaa
     6061 agccggagga tgtatttata tattcatttg gcggcagcgg aggccaaata agtttaaacg
     6121 aagcaataat aggtgttata aaaaaataca atggaagcag caatgtgaaa atcctccatg
     6181 tgacaggcag gaaacattat gacagcttca tggaagagct gaataagagt attaaaccgg
     6241 aaaaaaatat agaaattaaa gattatatgt atgaagcacc ccttgctctt tctgcttccg
     6301 atatagtaat aggaagtgcc ggggctataa caattgctga aataactgca ataggagtgc
     6361 cttcaatttt gattccaaaa acatatactg ctgaaaatca tcaggaatac aatgcccgtg
     6421 ctttggaaaa ggaaggtgcg gcattagtca tactggagaa agacttaagc gacaaaactc
     6481 ttcttaaatc aatcgaggaa atcattgaaa acaaaggcag attaaaaacc atgtccttga
     6541 attccaaaag gctgggaagt tctgacgttg aaaacaaaat ttatacaata atgacagatc
     6601 tcatcaattc acgaaagtaa caagtgaaaa aaatatgcat aacatataac atgaatcaac
     6661 ttgttgcgga gggataaaaa tgggaagcta tattgttgaa ggaagaaaca gtcttgaagg
     6721 aattgtggat atcggcggct caaaaaatgc cgcacttcct attattgcaa gcactgtaat
     6781 taccggaaga gaatacatcc ttgaaaatat accggatatt gaagacgtaa aagacatgct
     6841 tgaaatacta aacattatgg gatgcagcgt ccaactggaa aatggcattg cattaattga
     6901 taccaggggt ttaaatactc atgtagttcc cgaaaaatta gtaaggaaga taaggtcatc
     6961 aataatttta atgggagctg ttctgcagcg aacgggcaag gttgaagttg catatcccgg
     7021 aggctgcgaa ataggtctta ggcctattga tctgcattta aaaggtttaa gaaagcttgg
     7081 agttgaaata agtgaaaaaa atggtttgct gtcaggtata gtaaaagaac cggcaacctg
     7141 tgatattcat cttgactatc caagtgttgg ggcaaccgaa aatattatgc tgtttgcagt
     7201 aggaaacccc ggcaaaacaa ctatatataa ccccgcaagg gaacctgaaa ttgtagattt
     7261 gcagaatttt ttgaatactg ccggtttttg tatagatggt gcaggaacag gtcttattac
     7321 catagaaggc aaaaaaattg aaaatacaga tactgtagaa tacaaaatca tttccgaccg
     7381 tatagaggca ggaacttatt tgagtgcagc agcatcgatg aagagcaaaa tctacataag
     7441 aaatatagaa aaatctcatt ttaagtctat aacttctata tttgaagatg caggttgcga
     7501 aataagagaa aggcacggga ccataataat tgccaataac aaaaggttaa aagcgattga
     7561 aaaagtaaca actcagccct accccggctt tcccacggat atgcaggcac aggttatggc
     7621 atccatgacg gtagcagacg ggatttccat tattgaagaa acagtatttg acagcaggtt
     7681 taaacatgtt cccgagctcc agaaaatggg ggcatgcata acgaccatcg gcagggtggc
     7741 agtagttgat ggtattgaaa agctttcaag cgctgaggtt gaggcaaagg atttaagggg
     7801 cggggcagct cttgtaattg cagccatggg agctgaaggc acgtcaaaaa taaacaatat
     7861 tcatcatata aaaagaggat atgaaaaatt cttatataaa ctgcaaaact taggggttat
     7921 aataacagaa acttattaag aggagatggg gcacactgaa agagcaaggc agaatgtccc
     7981 cagcagagga ggggggacac acctccatgg agggtgagtc gctggagtaa taccatgaag
     8041 gaatgtcccc gttttatgga aaacaacaac aaaaaacaga ggaaaaagaa aaaaaagaga
     8101 aataaaaggc tattaataag tacagcagct tttattacgg ctgctgtagt tgttgttttt
     8161 tgtatttact atgtatatta tgaaacccct tatttcgacc ttatcggcgt tgacatggag
     8221 ggcaatctaa catatagtga tgaatatata atggagaaat caggtattga aacagggaaa
     8281 aaaatattcg atattgacag gcggcaggta aaagagaatc tagaaaatga agtatatgtt
     8341 gaaaatataa gggtagttta tacgctcccg aatagaatat ttcttgatat tgttgaaaga
     8401 gtggacaaat accaggtttt cataaacaat gaatacataa taattgacaa aaatggaata
     8461 gtattgagaa caagtactga taaatcggac ctattgacta ttgaaagtta tgcggaagta
     8521 gtatacaata taggagatag tattcaattt gcaggcattg aaaacattga agatgtattt
     8581 gatgcaatag aatatttaaa taatcaatat gggaccgacg caataaaaag ctgcagtgtc
     8641 gatcaggata acagtttttt atttaaaacc cggtatggaa ccggtgtaag gctggactta
     8701 agcgaagata ttaattatca gattgtattt gcgatgaaaa ttataaatga aagattaaat
     8761 aaaaatttaa ccgtatcatc aggattaatt gattttacta aaggggacag tcctgtatat
     8821 attgaggact aagggaggac gctaatgaac aaaatatggc agaaaacgat gatgtttgct
     8881 ataagcctgg tattaggaat agtcttgatt actcaaatga gaaccaagac cggcatactg
     8941 ggtatggtta atcttgaaga aaaatccctt gaacttaatg agaagcttgc acagttgaac
     9001 cttgaaaaaa cccggcttag agatgaattg gaagcactta aggaaaaaaa cagggaatat
     9061 gaagaaatat atgcagatgc tgaagaggag ctcataggac ttagtgaaga acttgaagat
     9121 caaaaaataa tatccggtta ttatgatgta aaaggtccgg gcaccataat taccatagac
     9181 gcagacccca attcatacat gagtctcgcg tccagccatc agtatatact ggcattaatc
     9241 agctatttaa ataatgcggg cgttgaggcc atatcaataa acgggcaaag atatacaaac
     9301 tatacggaaa tcgtaccggt tcttgagcac attaacgtga acggcgtggc tatggtactg
     9361 cctcttgaaa taaaggcaat aggaaactca gcgactataa attcttctct taactttgta
     9421 ggaggcatag taacccagtt caatgggatt ggatatataa ttgaaaccga agattcagat
     9481 gaaatattta ttcagcgtta tgaaggagaa aaacagttta agtatgctgt cccaatagcc
     9541 cgggaagaag aaatttaagg ggtggttatt ataaaaaaat cattcaatat aataattttt
     9601 atcttagttt ttttaacagc cgcagctctt gtaaacggtg cagatgcagg aatcaatgaa
     9661 gatagcattt acgaccttaa aaagctgaaa gaatatgaac gaagcaaaaa ggaaatggag
     9721 cttttagttg aaaacatgcg gaaagcggta aacagcgaaa aagataaaat tgcttactat
     9781 gaaaaaagag aatactttga tgatataatt gaaattaatt ctcaaattga ggagattaaa
     9841 ctatataatg gttttactga tgtgaggggt cccggtataa tgctcaggat ttcagacaac
     9901 atcaatgagg acgaaaacct tgatataatg gaaagaatag tacacgacgt ggatatagtg
     9961 gtcttgttaa acgatttgaa ggctgccggt gcagaagcca ttgaaattaa cggcaaaaga
    10021 ataaccaaca tgtcggaagt aatttgtgcg ggaccattga taagggtgaa taatgaggta
    10081 attcctgctc cttttgtaat aatagcaata ggagacatgg atgaactgta tgacgctgtt
    10141 actatggaag atacctatgc ctatgagcta atggatgtat acggaatgga agtagttgca
    10201 ataaaaggct ataatttaac tgtaagaaaa tttcgcggta ttaactatga accccaattt
    10261 gctgaaataa taaaagaaga ggtagaagaa tgatatatgt tgcagccggt ctgatcctgg
    10321 gtatagttgc aggactaaac ttaaatcttg tctacaatcc aaattacata gtttatatat
    10381 cactggcaat actggcggta ttaaatacta taatcaacat gctgcatgac agcaaaacta
    10441 cagaattgtc aatgggaaaa agcattgcat atttattgac tgacttactg tttgctttgt
    10501 tcctgggatt tgtaggcgag caatcaggcc tccccattta tttagcggct gtttttgcat
    10561 tcgggaataa tatttataaa aaattaagac tcctggttga tatttatctt gaaaagttta
    10621 gcaaaaacaa gtaaactaaa aatatatata tttattatta aatttatgat ataatcaaat
    10681 aaataatata aaaattctct ctcataggag gtcgctataa tgtttcaatt tgaagaacca
    10741 tcagaaaaac ttgcaaatat caaggttata ggtgtcggcg gaggcggtaa caatgcagtt
    10801 aacagaatga tagacgccgg agtcaggggt gttaatttta taagtgtaaa tacggacaag
    10861 cagcatctgt ccacatcaaa ggctgaaact aagtttcaaa tagggcaaaa gcttacaaat
    10921 ggtttaggtg caggctcaaa acccgaaata ggagaaaaag cagctgaaga aaacaaacag
    10981 gatattgcag aactggtaga aggcgcagac atggttttta taactgcagg catgggaggg
    11041 ggaaccggaa caggtgcagc tcctataatt gccgatattg caaaatccat gggcatactt
    11101 acggttgcag ttgtaacaaa gccctttatg tttgaaggaa gatgcaggat gaaaaatgcc
    11161 gagatgggtc ttaaacggct aaaggaaaat attgatactt tagttgttat tccaaacgac
    11221 aaactccttg agataacaga taaaaaaaca accatgcttg aagcctttgc catggcagat
    11281 gatgtattga gacaaggtat acagggcata tccgacttga tagcaatccc tgctataatg
    11341 aatcttgact tcgctgacgt tgaaacaatt atggccggtc agggtcttgc ccacatgggc
    11401 attggccagg caacaggaga aaacagagca ttaaatgctg ttaagcaggc aatacaaagc
    11461 ccgcttcttg aaacttccat caatggagca agaggagtcc tggtaaacat taccggcgga
    11521 ataagcatgg ggatccatga ggttaacgag gcagccaaac ttatcgaaga agctgctgac
    11581 cctgaagcaa acctgatata cggagcaaat attgacgagg ccctgggaga tacagtaaag
    11641 attacagtta ttgcaacggg ctttggagaa agcaaagcca aaaaaccaga gggtgaagcc
    11701 gctgaaagca atgaaaagaa accggaaagc cccttcgact ttacaatacc cagcttctta
    11761 aacaaaaaat agtaattatg gcaagaggac ggttcttcgg gaacaaaaag aaccgtccct
    11821 aaatatgtaa tataattttc caaaaaaaaa tagttgacat ttgaattctg atgtggtagt
    11881 ctttaaatgt attacagatg tatttatgga tttcacatgt ttttagatat tatatgtgg
//
LOCUS       DAOPUT010000047        11733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_5595_length_11733_cov_19.086487, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000047 DAOPUT010000000
VERSION     DAOPUT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5595_length_11733_cov_19.086487"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            810..1784
                     /locus_tag="PKK75_05425"
     CDS             810..1784
                     /locus_tag="PKK75_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNZ83073.1"
                     /translation="MYINYNDLCPYPLSDFLEYSIAIKGRADKTVNQYFLDLRTFYRF
                     LAVKFKLSPDIENFEEIDISPVSLDVIKKLKIMDLHSFIAYTDRERKNSNRTKARKMS
                     ALRSYFKYLHSVVKLIPENPALELETPKNKSRLPVALSLDESKSLLSSISGNNEKRDY
                     AIIMLFLNCGLRLSELISINTDKIKGDTLTVIGKGNKERTIYLNDVCVKAIEEYLEVR
                     QDPKKGHEKALFISNRRTRFSQKGVQHMLDKYLTEAGLSGKGYSPHKLRHTAATLMYQ
                     YGHVDIRALQEILGHESISTTQIYTHINKDLLRDAVKQNPLNDSAEDE"
     gene            complement(1866..2114)
                     /locus_tag="PKK75_05430"
     CDS             complement(1866..2114)
                     /locus_tag="PKK75_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ83074.1"
                     /translation="MFLAVLLISIITFTSIASINAYSKDIPRFDYVNVQQGDTLWSIA
                     SDYAASRDIRQVIYEISRLNNIHNSPIHPGDIIKIPLN"
     gene            2311..2934
                     /gene="lexA"
                     /locus_tag="PKK75_05435"
     CDS             2311..2934
                     /gene="lexA"
                     /locus_tag="PKK75_05435"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNZ83075.1"
                     /translation="MSYEGLSDKQIKIIEYIKDELTLRGYPPSIREICKAVGLSSTSS
                     VHAHLNTLEDKGYLKKGTNKRRALELIDVDDICSDMPKKEIINLPIIGTVTAGSPILA
                     VENIDDTLPISIDFVGNKESYVLKVKGDSMIEAGILHGDFVIVNSQHNAKNGDIVVAL
                     IGDEATVKTFYKEKDHIRLQPQNSSMDPILIKEPYILGVVKAVVRKY"
     gene            complement(3050..4327)
                     /locus_tag="PKK75_05440"
     CDS             complement(3050..4327)
                     /locus_tag="PKK75_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HNZ83076.1"
                     /translation="MFTENKLCKPFGISDKVYAYVIEVEKEIKKHFEKIEEIREYNQY
                     KVIHAMKEHRLDYTHFYWNTGYGYDDAGREKVEKIYASVFNTEDALVRPSIASGTHAL
                     YLTLSGILQHGDEVISISGRPYDTLLTVLGEEGNEPNNLREAGVIYKEIPLYNNDIDW
                     ESAVKAVSMKTKLLIIQRSTGYSFRPALTIEKIRAAIEKIRHLYPNIYIMVDNCYGEF
                     VDVIEPSDAGADVVVGSLIKNPGGGIALSGGYIAGKKILIDRIANRLTAPGVGKEIGL
                     TFGTTRNTLQGLFFAPHIVSEALKGALLFGAVYKSLGFEITPELDDERSDIIQAIKFN
                     NKDMVIKICEAVQAASPVDGHVSPIPWDMPGYTNQVIMASGAFVSGSSIELSADAPMR
                     PPYIAYIQGGLFYDHVKLGVMLSLEKMKELIDI"
     gene            complement(4478..4717)
                     /gene="hfq"
                     /locus_tag="PKK75_05445"
     CDS             complement(4478..4717)
                     /gene="hfq"
                     /locus_tag="PKK75_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNZ83077.1"
                     /translation="MASIINLQDTFLNSVRKEKITITLYLNNGFKLTGTVTGFDNYVI
                     VIETDKGQQLVYKHAVTSILPFKQIKLFTGDTEDQ"
     gene            complement(4731..5666)
                     /gene="miaA"
                     /locus_tag="PKK75_05450"
     CDS             complement(4731..5666)
                     /gene="miaA"
                     /locus_tag="PKK75_05450"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNZ83078.1"
                     /translation="MNKIIVIVGPTAVGKTHISVELAKKLGTEIISADSMQIYKGMDI
                     GTAKVTEDEKQGIIHHMIDIVSPDEDYSVSEFKYTAEKIIDRLLEEKKVPVIVGGSGL
                     YVNSLIYDLDFGNARSDEKLREYYTYYYKKHGEDALYDKLLKIDPEAAEKIHKKNVKR
                     VIRALEVYDITGSKFSEINTDIRKESNKYDCILAGLSMERKVLYERINQRVDEMLSKG
                     LLEEVRSLMEKGYCKNLVSMRGIGYKEIIDHLEGITDYDEAADILKRNTRRFAKRQYT
                     WFLKDRNVKWFSMDNPADVDNTINNILNYVRIRLN"
     gene            complement(5675..>6255)
                     /locus_tag="PKK75_05455"
     CDS             complement(5675..>6255)
                     /locus_tag="PKK75_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNZ83079.1"
                     /translation="PELSVIGILMDTYIICEDIEKNRMFMIDQHAAHERINYERFLNQ
                     YSAREILIQEIVPEVINLSFDDYETAVSNVEIFENLGISIENFGNNAILINNIPTIFN
                     NFNLRDVFYTILDSLKEAEKSNLDFIMDKIIKDACVMSVKSGDKLHMSEARALLNDLG
                     MTENPYTCPHGRPVIIKMTRYEIEKMFERIQS"
     assembly_gap    6256..6355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6356..7357)
                     /gene="mutL"
                     /locus_tag="PKK75_05460"
     CDS             complement(<6356..7357)
                     /gene="mutL"
                     /locus_tag="PKK75_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNZ83080.1"
                     /translation="MAAIHLLDNETINKIAAGEVIENPRSIVKELIENSIDAGADEIT
                     VEIKNGGKSLIRITDNGKGIERDQVENAFKRHCTSKISSSDDLNSLLTLGFRGEALAS
                     IAAVSRVELITRTNDDIYGLKMKIDGGEIILKEDVGCPVGTTVAVTDLFYNVPARKKF
                     LRSDTSESSNINEIVVSLALSKEYISFKYINNNYVAFRTPKTDNFINTISSLYEKDLY
                     NSLLKVDYKGDVVSITGYTTNLNYYRGNRKQQVVFVNGRFIKHRRMSFFVEAAYNTLL
                     PKNKYPACFLKIEIPPSLVDVNVHPAKTEIRFQNEEMVLNEVKRAVYSALHSINIMKE
                     "
     gene            complement(7517..10132)
                     /gene="mutS"
                     /locus_tag="PKK75_05465"
     CDS             complement(7517..10132)
                     /gene="mutS"
                     /locus_tag="PKK75_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ83081.1"
                     /translation="MANYTPMMEQYFEIKKQYQDCILLFRLGDFYEMFFDDAITASKV
                     LEIALTGRDCGQEERAPMCGVPYHAVDGYISKLIENGYKAAICEQLEDPALAKGIVKR
                     DVIRVITPGTIIDQAMLDEKTNNYLCCIYLDKGFGMSYVDISTGELHVTENISLNDFL
                     KNSTNDLLIEEINKIAPSEIISNKLTGNEPVDSMINLSEGLSLDECKEIVLKHFKLVS
                     LDSLGLKDNFHAVTSLGMLLNYLNETQKTSLDHINKLHFYKVGEYLQLDGSTRKNLEL
                     IETIRGKKGPGTLYHVIDNTMTAMGGRLLKKWIEEPLKNIKHINLRLDAVEELYKNVM
                     ASNNIKECLRSVYDIERLISRIVYGSCNGRDMNSLKQSLSNLPDLKAELSGLSSELFA
                     EIYSKFDELKDIFDIIDKAIVEEPPVSIKEGGILKTGYDDELDELKEITVNGKNWISG
                     LQTKERDATGIKNLKIGFNKVFGYYLEVTKSYLKDVPEYYIRKQTLANCERYVTPELK
                     EMEAKILNADEQIMKLEYELFLEIRQFISSQVKRIQETAYNIAVVDTINSLAIAAVKN
                     NYVRPEMNSKGYMSITDGRHPVIEKIMKNEMFVPNDTYIDNREYRMSIITGPNMAGKS
                     TYMRQVALIALLAHLGSFVPAKRAEICVVDKIFTRVGASDDLSQGQSTFMVEMSEVSN
                     ILQNATSDSLLILDEIGRGTSTYDGLSIAWSVVEYITKKIKAKTLFATHYHELSELES
                     KLKSVKNYKILIKEENDKITFLRKITEGSIDRSYGIQVANLAGLPEEVVGRAKEILRQ
                     LDESDINKPFARKKNRVNDNFQVSMFQPDTGEKGQNKEYRELTENIKNIDIENLTPMQ
                     SFKLLNDIIEKAKQI"
     gene            complement(10226..11647)
                     /gene="miaB"
                     /locus_tag="PKK75_05470"
     CDS             complement(10226..11647)
                     /gene="miaB"
                     /locus_tag="PKK75_05470"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNZ83082.1"
                     /translation="MKGRNTDIITEDVKLDDDKIIELKNNNEEFFIMKGRRKTYHILT
                     YGCQMNEHDSEKISGMLSSIGYEQTDDDKSADLIIFNTCLIRENAELRVFGKLGEVKG
                     LKRNNPDMLVAVCGCMMQKQEIREKVLKKFSFVDIIFGTNTIHELPMLIYNVEVNKRK
                     SVGIVDNTDSIYENMPKQRKFKHTALVNITYGCNNFCTYCVVPYVRGREKSREPNEII
                     DEIKALADDGCKEVTLLGQNVNSYGSNLDKKVTFADLLYEVNKIEGIELIRFMTSHPK
                     DLTDDLIRAIKECNKVCKHVHLPVQSGSSDVLFRMNRKYTKDHYLNLVEKLKAAVPDI
                     AITTDIIVGFPGETEEDFMETIDIVKKVQFDSAFTFLYSVREGTRAAIMDNQVPDDVK
                     HERFDRLLKVLYPIVLDKNEKFIGKVFPVLVESAENDKLTGRTEHFRLVHFKGSSDLV
                     GKIVNVKITNVKTFHTEGEIVEQ"
BASE COUNT         3370 a         2448 c         1855 g         3960 t
ORIGIN      
        1 actttttcga gagacctctt ctttttggaa tttcaacgtt attcttagtt ccttagacca
       61 attgataaaa tttctttgat aatttcaatt cacaaattta tatttcctgt aataattatt
      121 taataatttc ccgaccagaa aaactttttt tattatactt ctttttaaat tgtttcagct
      181 tccactgata tttttcaaag tatattattc cggactccaa tatatgatta taattggaac
      241 cataatagcc cctatgatta ggacatttta aattttttat acttaactaa aacgaatatt
      301 cacaaatata ttcaaagaac cctcaagtca agtcacaggc tcaaatttca aaaaaacacc
      361 ctgaaaacag ggcatttaaa tgcgttttaa cgaaagatat ttatcttctt gaccatttat
      421 accttctaaa tatatctcct tttgattaaa cccgcaaaaa agttagtttt tgaaatttct
      481 gtctgacaaa taataaaatt tgatcaattg tgctccctta ttaaataata aaaatttttt
      541 tgtccttccg gggcttatta tttgtaataa tggaaattat tatggattat attttgccgt
      601 ttatacgttt agctaaagat tttcaaaatc ttgcacgctt agcagtgcat agagtttata
      661 ttaatatctc gcaaatcgca aaacaccatt taaaagctaa tattaagaag aacaatgaaa
      721 ataagcttgt cttttttctt attcttctat ataattaatt atgataattt tgcgacattt
      781 ttgtatagac ttttataaag gagaccacca tgtacataaa ttataatgac ctctgccctt
      841 accccttgag cgattttttg gaatactcaa tagctatcaa aggcagagcg gataaaacag
      901 taaatcaata ttttcttgat cttcgaacct tctacaggtt tcttgcagtt aaatttaaat
      961 taagtcctga tattgaaaac tttgaagaaa ttgatatctc ccctgtttca ttggatgtta
     1021 taaaaaaact gaaaataatg gaccttcatt catttattgc ctatacagac agggaaagaa
     1081 aaaattcaaa cagaaccaaa gccagaaaaa tgtctgcgct taggtcatat tttaaatatc
     1141 tgcattccgt agtaaagctc attccggaga atccggcact ggaactggaa acaccaaaga
     1201 ataagagccg cctgccggta gcactatcat tggacgaatc gaaaagttta ttgtcatcaa
     1261 tatccggcaa caatgaaaaa cgcgactatg ccataattat gctgtttttg aactgcggcc
     1321 tcagattatc agagcttata agcatcaata ccgataaaat caagggagac accttaactg
     1381 tgatcggaaa aggcaacaag gaaagaacca tttatttgaa tgacgtatgt gtaaaagcta
     1441 tagaagaata ccttgaagtg agacaagatc ctaagaaagg ccatgaaaaa gcccttttta
     1501 taagcaaccg cagaaccagg ttctcacaaa aaggagttca gcacatgctt gacaagtacc
     1561 tgaccgaggc cggcttatcc gggaaaggat actcccccca caagctccgc cacacggcag
     1621 ccactctaat gtatcaatac ggccatgtgg atatcagggc tctgcaggaa attttaggcc
     1681 atgaaagcat ttcaacaaca caaatatata ctcatatcaa caaggacctg ctccgggacg
     1741 cagtaaaaca aaatccgctt aatgattcag cggaagatga ataattcaat aatttcagcg
     1801 atactaacat acatttttca aaaataaaat cctatatgta ataatatagg atttcagctt
     1861 agtatttagt ttaagggaat ttttattatg tctccggggt gaatcggact gttatgtatg
     1921 ttgttaagcc gggatatttc atatatgacc tgccgaatat ccctggaggc ggcataatca
     1981 gatgctatgg accacaaggt gtcgccctgc tggacattta cataatcgaa ccgtggtata
     2041 tcctttgaat atgcatttat tgaagctatt gaggtaaaag taataatact tatcagcaag
     2101 acggccagga acataaaacg tttaaatttt ttaacattac tcactttata tttatttaat
     2161 attaccataa cccacctcga acatattttc tattttcatt ctaacagaac attagttcga
     2221 tgtcaacata ttttttgcat tttttcaaac aaatgtttgc tttacctgtc aaactatgtt
     2281 aaaatataaa aaaacaagcg aggtggaatt atgagttatg aaggcttaag cgacaagcaa
     2341 attaaaataa tagaatatat aaaagatgaa ttaactttaa gaggttatcc cccttctatc
     2401 cgtgaaatct gcaaagctgt aggtttaagc tctacttctt ccgtccatgc ccacttaaat
     2461 acattagaag ataaaggata cttaaaaaaa ggaaccaaca aaagaagagc tctggaattg
     2521 attgatgttg atgatatatg cagcgacatg ccaaaaaaag aaatcataaa tttaccgatt
     2581 ataggaaccg ttactgccgg ctcccctatt ttagccgttg aaaacatcga tgacaccctg
     2641 cctatatcga ttgattttgt aggtaataaa gaatcgtatg tcctgaaggt aaagggagac
     2701 agcatgattg aggcaggaat attgcatggg gattttgtaa tagtcaattc ccaacacaat
     2761 gccaaaaacg gcgatattgt ggttgcctta atcggtgatg aagctacagt caagaccttt
     2821 tataaagaaa aggaccacat ccgcctgcag cctcaaaact cttccatgga ccccatatta
     2881 ataaaagagc cctacatctt gggagttgta aaggcagttg taagaaaata ttagcagata
     2941 aatttcaaat aaataaataa taaagcagtc ctcctgtttt aaattcagga gactgctttt
     3001 ttgataattc catattaaat gtttctatga aaagacttcc atccccttgt catatatcta
     3061 tgagttcttt catcttttcg agtgaaagca taacccccag ttttacatgg tcgtagaaca
     3121 agcctccctg gatataggcg atataaggcg gtctcatggg tgcatctgca cttagctcaa
     3181 ttgatgagcc ggaaacaaaa gctcccgaag ccattataac ctggttagtg taaccgggca
     3241 tatcccatgg tatgggtgaa acatgaccgt caacgggaga tgccgcctgg acagcttcac
     3301 atattttaat taccatatcc ttgttgttaa attttattgc ctggataatg tcgcttcttt
     3361 catcatctaa ttccggagta atttcaaatc caagagactt ataaactgca ccgaacaaga
     3421 gtgcaccctt taacgcttca cttacaatat gaggtgcaaa aaacaaaccc tgcagcgtat
     3481 ttctcgtagt tccgaaggta aggcctattt cctttcccac tccgggagct gtgagacggt
     3541 ttgcaattct gtcaattaag atctttttgc cggcgatata gccacctgac agcgcaattc
     3601 cgccaccggg gtttttaatc agggacccca caaccacatc agcacctgca tctgaaggct
     3661 ctataacatc cacaaattct ccgtagcagt tatcaaccat tatatatata ttcgggtata
     3721 aatgacgtat tttttctatt gcagctctta ttttttctat tgtaagtgca ggtctgaagc
     3781 tgtagcctgt tgagcgctga attattagca gctttgtttt catggaaact gcttttactg
     3841 cgctttccca gtctatgtca ttgttgtaaa ggggtatttc cttgtaaatt acacctgctt
     3901 cacgcagatt attgggctca tttccttctt ctcccaaaac tgtaagtaaa gtatcatagg
     3961 gacgtccgga aattgatata acttcatccc cgtgctgcaa tattcccgac agtgtcaaat
     4021 aaagtgcatg ggtacctgaa gcaatggaag gccttacaag ggcatcctcg gtattgaata
     4081 cggaagcata tattttttca accttttccc ttcccgcatc atcgtagccg tagccggtat
     4141 tccaataaaa gtgagtataa tcaagtctgt gttccttcat tgcatgaata accttatact
     4201 gattatactc tcttatttct tcaattttct caaaatgctt cttaatttct ttctctacct
     4261 ctattacata ggcgtaaact ttgtcactga tgccaaatgg tttacataat ttattttctg
     4321 taaacataat tctccttcgt aaaatattaa aatatgcagg ttttctactg ctagatatac
     4381 ataaataaaa ttatgtatac ctttctctca attaataata aatcttggtt tacataattt
     4441 aatttctgta aaccttatta ttccttttct attaaactta ttggtcctct gtatctcctg
     4501 taaacagttt tatctgttta aagggcagaa ttgatgtgac tgcatgctta taaaccaatt
     4561 gctggccctt gtctgtttcg ataacaatta cataattgtc aaagcctgtc accgttcccg
     4621 tcagtttaaa tccattgttt aaatatagcg ttatagtgat cttttctttt cttacgctgt
     4681 ttaaaaacgt atcctgtaaa ttaataatac tcgccatttc ccttctcctt ttaattaagc
     4741 cttattctta cataatttaa aatattattt atagtattgt caacgtctgc cgggttatcc
     4801 atgctgaacc atttaacatt cctgtcctta agaaaccatg tatactgcct tttggcaaat
     4861 ctcctggtat ttcgttttaa aatgtccgct gcttcatcgt aatccgtaat gccttcaaga
     4921 tggtcgataa tctccttata gcctattcct ctcatggata caagattttt acaataacct
     4981 ttttccataa gcgaccgtac ctcttccaag aggcccttag aaagcatttc atccactctt
     5041 tggtttattc tttcatagag gacttttctt tccattgaaa gccctgccaa tatgcagtca
     5101 tacttattgc tttcttttct gatatcagtg tttatctcgg aaaatttcga ccctgtgatg
     5161 tcatatactt ccagtgccct gataaccctc tttacgtttt tcttatgtat tttttccgct
     5221 gcctccgggt caattttcaa tagcttgtca taaagtgcat cttcaccgtg cttcttgtaa
     5281 taataagtgt aatattccct taatttttca tcagacctgg cattgccaaa gtccaggtca
     5341 tatatcagag aatttacata caacccgctt ccccctacaa ttaccggaac cttcttttct
     5401 tccagaagcc tgtcgattat tttttcagcc gtatatttaa attctgatac cgaataatct
     5461 tcatcaggac ttacaatgtc aatcatatga tgaattatgc cctgtttttc atcctcggtt
     5521 acttttgcag ttcctatatc cattcccttg tatatctgca ttgaatccgc tgatattatc
     5581 tcagttccaa gcttttttgc aagttctacc gatatatggg ttttccccac agctgtaggc
     5641 cctacaatta caattatctt attcatatct tttcctaact ttgaattcgc tcaaacatct
     5701 tttcaatttc gtacctggtc atcttaatga ttacaggtct cccgtgagga caggtatacg
     5761 ggttttctgt cataccaagg tcatttaaca aggctcttgc ctcagacata tgaagcttgt
     5821 cgccggattt cacagacatg acgcatgcgt ccttgataat cttatccata ataaaatcca
     5881 gatttgactt ttctgcttct tttaaggaat ccagtatggt gtaaaacaca tcccttaaat
     5941 taaaattatt gaaaattgtc ggtatgttat taataagtat agcattgttt ccaaagtttt
     6001 caatggaaat tcccaaattt tcaaatattt caacgttgga aacagccgtt tcgtaatcat
     6061 caaacgacaa attaattact tcaggaacta tttcctgtat taaaatttct cttgcagaat
     6121 actggtttaa aaatctttca tagtttattc tttcatgtgc cgcatgctgg tcaatcataa
     6181 acatccggtt tttttcaata tcttcgcaaa ttatgtatgt gtccattaaa attccgataa
     6241 cagaaagctc cggcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcct
     6361 tcatgatatt aatggaatga agagccgaat aaaccgccct tttaacttca tttagaacca
     6421 tttcttcatt ttgaaacctt atttccgtct ttgcaggatg aacatttaca tccaccaggg
     6481 atggaggaat ttctattttt aaaaaacatg caggatattt gtttttagga agtaatgtat
     6541 tgtatgccgc ttcaacaaaa aaactcatac gcctgtgttt tataaacctg ccgtttacaa
     6601 aaacaacctg ctgcttcctg tttcccctat aatagtttaa atttgttgta tagcccgtta
     6661 tactcacaac atcgccctta taatcaacct ttaaaagtga attatatagg tccttttcat
     6721 acaagctgga aatggtattg ataaaattat ctgtttttgg agttctaaag gcaacatagt
     6781 tattatttat gtatttaaat gaaatatact ccttgcttaa agcaagggac acaacaattt
     6841 cattgatgtt tgagctttcg gacgtatcgc tcctaaggaa cttcttcctg gcaggcacat
     6901 tgtaaaatag gtcagtaact gcaactgtgg tccctacagg gcatcccaca tcctccttta
     6961 aaatgatttc tcccccgtca attttcattt taagaccgta aatatcatca ttagtcctgg
     7021 ttataagctc aacccttgaa acggcagcta tgcttgccag ggcttccccc cgaaacccca
     7081 atgtcaaaag agaatttaag tcatcagagg aagaaatttt gcttgtacag tgtcttttaa
     7141 aagcattttc cacctggtcc ctttcaattc ccttcccgtt atcggttata cggatcaagc
     7201 ttttgcctcc gtttttaatt tcaacggtta tttcatcagc tcccgcatca atagagttct
     7261 cgattaattc ctttacaatt gatctggggt tttcgataac ctctccggcg gctattttat
     7321 taatggtttc attatcaagc agatgtattg cagccatatg cttacattcc tttccttttg
     7381 ctataaatat gccttttata agtgtcattc aatgggtact aaacttatac gaattaagca
     7441 gctgataaat tttaatgtaa agctgcggat ctcaaaaggt gctattatta taattgcttc
     7501 attcaatgac atacagctat atctgttttg ctttttctat aatatcattc agcagtttaa
     7561 atgactgcat gggtgtcagg ttttctatat caatattttt aatattctcg gttaattccc
     7621 tgtattcctt gttttgcccc ttttctccgg tatcaggctg aaacattgaa acctggaaat
     7681 tgtcgttaac cctgtttttc tttctggcaa agggcttgtt tatgtcgctt tcatcaagct
     7741 gccttaaaat ttccttggcc ctccccacaa cctcttccgg aaggccggcc aggtttgcta
     7801 cctgaatgcc gtagctcctg tctatgcttc cctctgtaat tttcctcagg aatgttattt
     7861 tgtcattttc ttcttttatt aaaatcttgt aatttttaac acttttcagc ttgctctcaa
     7921 gctcggacaa ttcatgatag tgagttgcga aaagtgtttt tgcttttatt ttcttcgtaa
     7981 tatattccac tacgctccat gctatgctca agccgtcata ggtgcttgtg cctcgtccga
     8041 tttcatccaa tatcaacaaa ctgtcagatg tggcattctg aagtatattt gaaacctcgc
     8101 tcatttctac catgaaggtg ctctgcccct gtgacagatc atccgaagct ccaacccggg
     8161 taaatatttt gtcaacaaca caaatttcag cccttttagc aggaacaaaa cttcctaagt
     8221 gagccaaaag cgctatcagg gcaacctgcc tcatataggt tgacttgcct gccatatttg
     8281 gacctgtgat tattgacata cggtattccc tgttatctat atatgtgtca tttggaacga
     8341 acatctcatt tttcattatc ttttcaatta caggatgcct gccgtcagtt atgctcatat
     8401 aacccttgct attcatttct ggccgcacat aattattctt tactgcggca attgcaagtg
     8461 aatttatcgt gtcaaccacg gcaatattat aggctgtttc ttggatcctt ttaacctggc
     8521 tgcttataaa ctgtcttatt tccaaaaaca gctcatattc aagcttcata atctgttcat
     8581 cggcattgag tatcttggct tccatttcct tgagctcagg agttacatac ctttcacagt
     8641 ttgccagagt ttgttttcgt atgtaatatt cgggaacatc cttcaaatat gattttgtca
     8701 cctccagata gtagccaaaa accttgttga aaccgatttt taagttcttt atgcctgtag
     8761 catccctttc cttggtttga aggcctgaaa tccaattctt gccgtttacg gtaatttcct
     8821 taagctcatc cagctcgtca tcataaccgg tttttagaat tccgccctct ttgattgaaa
     8881 caggaggctc ttctacaatg gctttgtcaa ttatatcaaa aatatccttc agctcgtcaa
     8941 atttcgaata aatctcggcg aacaattcag acgaaagccc tgacaattca gcttttaaat
     9001 ccggcagatt tgacagggac tgcttcaggg aattcatgtc cctaccgttg caagagccgt
     9061 atacaatccg gctgatgagt ctttcaatat catagacgct tcttaagcat tccttgatat
     9121 tgttggatgc cattacattt ttatataatt cttcaactgc atcaagccgc agattaatat
     9181 gcttaatatt tttaaggggt tcttcaatcc atttctttaa aagcctgcct cccatggcag
     9241 tcatggtgtt gtcaataacg tgatacaggg tgcccgggcc ctttttcccc cgtattgttt
     9301 caataagctc taaatttttc ctggtgctgc cgtcaagctg caaatattca cccaccttgt
     9361 aaaaatgaag cttattaata tggtcaaggg aggttttctg tgtttcgttt aaataattta
     9421 agagcatgcc aagagatgta acagcatgga aattatcctt aagtcccaat gagtccaggg
     9481 aaacaagctt aaaatgcttc aatactattt ccttgcattc atcaaggctt aaaccttcac
     9541 ttaaatttat catcgaatca acaggctcat ttcctgtcag cttattggat ataatttctg
     9601 agggagctat cttgttgatt tcttcaatta acagatcatt ggtgctgttt tttaaaaaat
     9661 cattcagtga aatattttcc gttacatgga gctctcctgt ggaaatgtcc acataggaca
     9721 tgccaaagcc cttgtccaaa taaatacagc acaaatagtt gttggttttt tcatccagca
     9781 tagcctggtc gataatggtt ccgggagtaa ttacccttat aacatcacgc tttacaatac
     9841 cttttgcaag agcggggtct tcaagctgtt cgcagatagc agccttgtag ccgttttcta
     9901 taagctttga tatatacccg tccactgcat gataaggaac tccgcacatg ggggctcttt
     9961 cttcctggcc gcagtctctt cccgtaaggg ctatttccaa aacctttgac gccgtaattg
    10021 catcatcgaa aaacatttca tagaaatccc caagcctgaa caaaagaata cagtcctgat
    10081 actgtttttt aatttcaaaa tactgctcca tcataggtgt ataatttgcc attattctct
    10141 ccctgattca aaagatgtta tcctttggag acatttttaa aagcagctgc ttattgaacc
    10201 gaccaataaa gtttagtcct gatgcttatt gctcaacaat ctccccttcc gtatggaagg
    10261 tcttaacatt agttattttt acatttacta ttttacctac caggtcactg cttcccttaa
    10321 aatgaaccag tctgaaatgc tccgtcctac ctgtcagttt atcattctca gccgattcaa
    10381 ccaatacggg gaatactttt cctataaact tttcattttt atcaagcact atggggtaca
    10441 aaactttcaa cagcctgtca aagcgctcat gctttacatc atcaggcacc tggttatcca
    10501 ttatggctgc ccttgttccc tctcttacag aataaagaaa agtaaaagca gaatcgaact
    10561 gaaccttttt aacaatatct atggtttcca taaaatcttc ctcggtttca ccggggaagc
    10621 cgacaattat atctgttgtt atagcaatat caggtactgc cgcctttaat ttttcaacca
    10681 gattcaaata atggtctttg gtgtatttac ggttcatgcg aaaaagaaca tcactgctgc
    10741 ctgactgaac aggcagatgc acgtgcttgc ataccttgtt gcattccttt attgccctta
    10801 tcaaatcatc agttaaatcc ttgggatggg atgtcataaa gcgaatcagc tcaataccct
    10861 cgattttgtt tacttcataa agaaggtctg caaatgtaac cttcttatcc aaattgcttc
    10921 cgtaggaatt gacattttgt ccaagcaggg ttacttcctt gcagccatca tctgccaatg
    10981 ctttaatttc atcaattatt tcatttggtt cccttgactt ctctctgccc ctgacatagg
    11041 gaactacaca gtaggtacag aaattattac agccgtatgt tatgtttacc agtgccgtgt
    11101 gtttaaactt cctttgtttg ggcatatttt catatataga gtcagtattg tccacaatgc
    11161 ccacactctt tcttttattg acttcaacat tataaattaa cattggcaat tcatggattg
    11221 tgtttgtacc aaaaattata tctacaaaag aaaatttctt tagtaccttt tctcttattt
    11281 cctgtttttg catcatgcag ccgcatactg ccaccagcat atccggatta tttcgtttta
    11341 acccttttac ttcccccagc ttcccgaaca ctctaagctc agcattttct cttatcaagc
    11401 atgtgttaaa tattataaga tcggcacttt tatcatcgtc agtttgttca tagccgattg
    11461 acgaaagcat tcctgatatt ttttcggagt cgtgctcatt catctggcag ccgtaggtca
    11521 agatgtgata tgtttttctt ctgcccttca taataaaaaa ttcttcgtta ttgttcttta
    11581 actctattat tttatcatcg tctaatttaa catcttccgt tattatgtct gtatttcttc
    11641 ccttcattgt cttccaactt tctaatttaa cattttagca tcttgagtat tataacattt
    11701 tacccgcttt tgttcaataa aaaaattccc ctg
//
LOCUS       DAOPUT010000048        11667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_5628_length_11667_cov_21.298226, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000048 DAOPUT010000000
VERSION     DAOPUT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5628_length_11667_cov_21.298226"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1586
                     /locus_tag="PKK75_05475"
     CDS             81..1586
                     /locus_tag="PKK75_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ83083.1"
                     /translation="MKTTKRIFAVLMIALLITSCSAQENTPISKDVKTDLTMGYTTEP
                     EGLDPHRTAAASTFTVTNNIYDTLVGVTPEWTTEPRLAKSWVISDDGMEITFTLRDDV
                     YFHNGRQMKARDVEYSFNRLKDAESPKARDYANITAIEVLGDYEIKFTTGTLDVELLK
                     SFVYPWAAVVPEEEVENLKTKPVGTGAFTLGEWVPQQYLTLKRNDNYYGDDVKLETVK
                     MVLIPDATGMMAGFQVGDLDIIPLTGDQVTMVEGNENYKIISEPMNAVQIMSLNTNNE
                     YLSSEKVRQAMAMAINKDEVIEASMFGYGAKIGSHLPPTSPDYYDTNNIIEYNPERAK
                     ELLKEAGYENGFEIDMSLPKNYQLHVDAGQVIADQLGRIGIDVNIQLIEWGTWLSEVY
                     GAKNFDSTVVGHTGRLDSYAFLSRYKSTSNDYISLTTGEVDELLEDALHELDEDKRKE
                     IYKEIQVILANKLPAIYLQTPHTMLALQKNVEGMEIFPIDFYDFKEVYFTK"
     gene            1612..2568
                     /locus_tag="PKK75_05480"
     CDS             1612..2568
                     /locus_tag="PKK75_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83084.1"
                     /translation="MLSYVLDRFKSSIVVLLVVSIITFFVLMIIPGNPAQLILGTDAT
                     PEAIAELHAAMGLDRPMYQQYLSWLLNLFKLDMGTSYVYGESVTALIARSIPVTFSIS
                     TFAMAIAVFIAFLFGMTSAIKKNSVIDYFSRSIMQLGAAIPSFWIGMVFIVYFGVKLK
                     VLPVSGFVPIERNLAGFIRSIFLPSLVLAIGEVGTLLRIIRSSMLDSLKQDYMDMARV
                     KGLSAGKTYIKYALRGALTAPLTIMGMQFAKLAGGTVVVETIFALPGIGRLVLTAVEH
                     RDIVLLQGLVMFITSTVVFITLIVDILVMFLNPRIKSFQEGE"
     gene            2568..3389
                     /locus_tag="PKK75_05485"
     CDS             2568..3389
                     /locus_tag="PKK75_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83085.1"
                     /translation="MRINLSLIIGIILVLIIIAVWVLSFVYMPYPPNEMNISNRFLKP
                     GIDSEHILGTDNFGRDILSRIMYGSRNVLLVGIVSVTLGTMIGILLGALAAMLKHYSD
                     KIIMRIMDGLMAFPGILLAMMLVTVLEKGIQGSIAAISIFMVPVFSRLVYSMILDTEN
                     LLYIKAAKSYGSSKGQIFFRHYIPAMLPRLITQFSSSIGSAVMIETSLSFLGLGIQPP
                     FASWGLMLSESRQFFMGYPYLAVAPGIAIIITVLGFNLIGDGLNDILIDREGSDE"
     gene            3382..4332
                     /locus_tag="PKK75_05490"
     CDS             3382..4332
                     /locus_tag="PKK75_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ83086.1"
                     /translation="MNKTVELKNLHIKAGDNTLVNKTSLTIEEGEIFGLVGESGSGKT
                     LTAKAIIDLLSEGLSYRADEIITAGKNMLTASREEKREHIGKNIGYIPQNTVFYLHPM
                     IKIKNQIGDGYIHHMNKTRKEALEKAETLLSKVGFKSPRTVLNYYPWQLSGGMRQRCN
                     IAMALMNDPKLIIADEPTTALDTTVQRQVMELFRTINEEMKLSILLISHDLGLVKNYS
                     HSLGVMYAGQIVESGYTNTVFSNPRHPYTRLLIKIIPSLKIKKSQPLTEIPGFVKDKG
                     RDGDGCLFYNRCPQAMDICKKYAAEHIDDKMDHYYRCNLE"
     gene            4343..5140
                     /locus_tag="PKK75_05495"
     CDS             4343..5140
                     /locus_tag="PKK75_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ83087.1"
                     /translation="MANKVVISAVNIRKEYVTKKHFLTGKTKEKYVAVKGAAFNIEEG
                     KTLGIVGESGSGKSTIGEIVGGLQKPTYGNVLYFGKNINELSKEEYLDYRKSVQFIFQ
                     DPKGSMDHNYKIFDILSMPLLTAKIARDKNEAEKLIDEMLMRAGLDLSIKKKYPTEIS
                     GGQCQRAAIARALLVNPKLIICDEPVSALDVSVQAQILNLLRELQRQLNISYIFISHD
                     IGVVNYMSDTILVMQNGTIVERGEAKDVLLRPKEAYTKKLIESAFMN"
     gene            5376..6122
                     /locus_tag="PKK75_05500"
     CDS             5376..6122
                     /locus_tag="PKK75_05500"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HNZ83088.1"
                     /translation="MEVLLKENEKIEDLQCKGLKIIQNKKWFCFGMDAVLLTNYCDIR
                     KNSKIVDLGTGTGIIPILLSGKRNYSKAYGLEIQAEIAEMAQRSVKLNSLQEKIEILN
                     IDLKDALEYLDADSFDAVISNPPYKLYNSGIINPDDKKAISRHEIKCSLEDVISTASK
                     LLKQYGRFYMVHRPDRLADIMCLLRKHRLEPKQIRFVHPKVKAKPNMILIRASKNGNP
                     ELKFDPPLYIYDEEGNYTDDVYQIYGMVNS"
     gene            complement(6362..7897)
                     /locus_tag="PKK75_05505"
     CDS             complement(6362..7897)
                     /locus_tag="PKK75_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ83089.1"
                     /translation="MEYPRHSYWHDTAENIKNPQVKNASLNVDLNNKEFDILIIGAGI
                     TGLTTAYLLKDLGLNIGIIEATSAGYGVTGFTTAKITIQHDLFYDNLINNFSFEEAKQ
                     YLKANEEGVKLIKKIIVENNIQCDYKDQDAYVYAYSIDEVDDLKKELKAYEKLGINGF
                     YTDTVPLPNKAYGAIGVKNQGQFNPLKYLYSLYNILSTANIAIYENVRALDINPHDDH
                     VDVKTEKGTIHAGKVIAASHYPFDNSFGLYFLRLYQEKSYVIAARTKEQPFGGMYINI
                     KDPIYSMRYHFSDNENLLILAGGNHRSGEKDDESECYNELERFLNSNFKNPEIVSKWS
                     TQDCMTYDKIPYIGRYSDSIDNLYVATGFKKWGMSHSAASALILRNKILCIEDDYSGI
                     FNPSRFTPVQSAKEFVSSVKSIALGFLNRLAGDLEEFSDVKPGEGKIVDYDGKKVGVY
                     KNEAGEYYCINPVCSHLKCALSFNEAEKTWDCPCHGSRYDINGHILEGPAVLPIDKIR
                     IKK"
     misc_binding    8074..8285
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8494..11616
                     /gene="ileS"
                     /locus_tag="PKK75_05510"
     CDS             8494..11616
                     /gene="ileS"
                     /locus_tag="PKK75_05510"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNZ83090.1"
                     /translation="MKLFKDISKADYRNNEHEISKYWEEIDLLEQSIETRKNKPSFVF
                     YEGPPTANGKPGIHHVTARTLKDSVCRYKTMKGYQVKRKAGWDTHGLPVEIEVEKQLG
                     LSTKQEIEEYGIAEFNKKCRESVFTYEAHWREMTERMAFLIDMDDPYITLENDYIESE
                     WWILDKVFKDGLIYEGYKILPYCPRCGTGLASHEVAQGYEEIKTMSAYVAFKRKDADE
                     YFLAWTTTPWTLLSNVSLTVNANMDYVKIRTKDKEDVKGKVYILAKDSAPRVINEEYE
                     VLEVKTGKELEFMEYEQLMPFVEVPEGKKSFYVTLADYVTIEDGTGIVHTAPAFGEDD
                     YNTGMLYGLPVLNPVDDTGRFRGTPWSDMFVIDADQPILKWLNENGVLYKKEIFAHNY
                     PHCWRCHTPLLYYARPSWYIQMTKLKDLLVSNNNTVNWFPDYVGEKRFGNWLENVNDW
                     AISRSRYWGTPLPIWRCECGHLESIGSRKELVEKAVEEIDESIELHRPYVDEVHIVCP
                     KCGKHMTRVKDVIDCWFDSGAMPFAQWHYPFENKERFGELFPAEFINEGIDQTRGWFY
                     SLIAVSTLVTGKTPYRNVLVNDLVLDKFGKKMSKSRGNTVEPMDLFDRYSVDALRWYL
                     LYVSPVWTPTRFDEDGLKEILSKFIGTIKNVYTFFALYANTDSIKPQDYFVEYRDRPE
                     LDRWIVSKYNNLVKYVTESMDVYDHNKSVRAIQNFVNEDLSNWYIRRSRRRFWETELT
                     EDKKAVYVTTYEILVGISQLIAPFAPYLSEEIYRNLTGQPSVHLSYFPEAKEELIDEN
                     IEKRMDLVKDIVSLGRASRENAKIKVRQPIQKIHVDGKYKQLIDDLVPLIKEELNVKE
                     VVFEHDLNEFMDFSLKPNFKIAGPMLGNNVKAFAGILAKADALNIITKVQSGEKCELD
                     VNGQIVELDNQLLDIRISAKEGYDVATGNNLFTIIDTNLTEELLNEGYAREFISKVQQ
                     MRKNNGYEMMDRIEIYYTSSPEIDKAVNDYEDFIKEETLADLISVSEEDMEVQNLNGV
                     DTGMKLERIR"
BASE COUNT         3815 a         2002 c         2425 g         3425 t
ORIGIN      
        1 gattataaaa caggtatatt attattggtt ttcagtaaaa gatattctaa ggaaatttta
       61 ttagctgaaa ggaagaagaa atgaaaacaa ccaagagaat ttttgccgtg cttatgattg
      121 cactgctgat tacatcatgc tcagctcaag aaaacacgcc tatttcaaaa gatgtcaaga
      181 ctgatttaac aatgggctac actactgagc cggaaggtct tgatcctcac agaactgccg
      241 cagcctccac atttactgta actaacaata tatatgacac tttggtgggc gtaacgccgg
      301 agtggacaac tgagcccaga cttgcaaagt catgggttat ttctgatgac gggatggaaa
      361 ttacttttac attgagagat gacgtttatt ttcataacgg aagacagatg aaagccagag
      421 atgtggagta ttctttcaat agacttaagg atgccgaaag ccccaaggca agagattatg
      481 caaatattac agcaattgaa gttttgggcg attatgaaat aaagtttaca acagggacat
      541 tggatgttga gcttttaaag agctttgttt atccatgggc ggctgttgtt cctgaagagg
      601 aagtcgaaaa cttaaaaaca aagcctgtgg gaacaggagc ctttacactg ggggaatggg
      661 ttccccagca gtatttgacc ttaaaaagaa atgataatta ttacggggat gatgttaaac
      721 tggaaacagt taagatggtt ttaatacctg atgctacagg catgatggca ggcttccagg
      781 tgggagacct tgatattata cctctgacag gagaccaggt tacgatggtg gagggaaatg
      841 aaaattacaa aataatttcc gagcctatga atgctgttca aataatgtct cttaacacaa
      901 ataacgaata tctttccagt gaaaaagtaa gacaggcaat ggcaatggcc atcaataaag
      961 atgaggttat cgaagcatcc atgtttggtt atggcgctaa aataggctct cacctgccgc
     1021 ctacttcacc ggattactat gatacaaata atataattga atataatcct gaaagggcta
     1081 aggagctttt gaaggaagca ggatatgaaa acggatttga aatcgatatg tccctgccaa
     1141 agaattatca gctccatgtg gatgcagggc aggtaattgc agaccaattg ggaagaatag
     1201 gcatagatgt gaacatccag cttattgagt ggggaacatg gcttagtgag gtatatgggg
     1261 cgaaaaattt tgactccacg gttgtaggcc acacgggaag acttgattca tatgcatttc
     1321 tttccaggta caagtctacc agcaatgatt atataagcct gacaacaggt gaagttgatg
     1381 agctgttaga agatgccctt catgaattgg atgaagataa aagaaaagaa atttacaaag
     1441 agatacaggt aattttagca aataagcttc ctgcaattta tctgcagact ccccacacaa
     1501 tgctggcgct gcagaagaat gttgaaggaa tggagatatt ccccatagac ttttatgatt
     1561 ttaaggaagt gtattttaca aaatagtgta aatcttcctt aagaggtgca tatgttaagt
     1621 tatgttttag acagatttaa aagttccata gttgttttgc ttgtagtgtc gattattacg
     1681 ttttttgtat tgatgataat acccggaaac ccggcgcagc tgatattggg tactgacgcc
     1741 acccctgaag ccatagccga gcttcatgcc gctatgggac ttgacagacc catgtatcag
     1801 caatacttaa gctggctttt gaatcttttt aaacttgaca tgggcacatc ttacgtatac
     1861 ggcgaatcgg ttactgcctt aatagcaaga agtattcccg taaccttttc aatatcaact
     1921 tttgcaatgg caattgcagt ctttatcgcc tttctttttg gtatgacttc tgcaattaaa
     1981 aaaaacagcg taattgatta tttttcaaga agcataatgc agcttggagc agccatccct
     2041 tctttctgga tagggatggt ttttattgtt tatttcggcg ttaagcttaa agtactccct
     2101 gtttcagggt ttgttcccat tgaacgaaac ctggcaggat ttataaggag tatttttctg
     2161 ccctcccttg ttcttgcaat cggcgaagtt ggaacgcttt tgagaattat cagaagctcc
     2221 atgctggatt cattaaaaca ggattatatg gacatggcaa gggtaaaagg tctcagtgcc
     2281 ggaaagactt atataaaata tgccttaaga ggcgcattga ctgctccctt gacaataatg
     2341 ggaatgcagt ttgccaagct tgccggaggg acagtagtgg tagaaaccat atttgctctt
     2401 cccggaatcg gaagactggt tcttaccgct gttgagcaca gggatattgt gcttttgcag
     2461 gggcttgtta tgtttattac atcaacggta gtctttatca cattgattgt tgatattttg
     2521 gttatgtttt tgaatccaag gattaagtcc ttccaagagg gggaataatg agaataaatt
     2581 taagcttgat tataggaatt atattggtct tgattataat agctgtttgg gtcctttcgt
     2641 ttgtatatat gccgtatcct ccaaatgaaa tgaatatttc aaacaggttt ttgaaacccg
     2701 gtattgactc agagcacatc ctggggacag ataatttcgg cagagatatc ttaagccgca
     2761 taatgtacgg ttcaagaaat gtacttttgg tggggatagt ttctgttacc ctgggaacca
     2821 tgatcggaat acttttgggt gctcttgccg ccatgcttaa acactattcg gacaaaatta
     2881 ttatgcgtat tatggacggg cttatggcat ttccgggcat acttcttgca atgatgctgg
     2941 ttactgtttt ggaaaaagga attcaaggct caattgcagc tatcagtatt tttatggtgc
     3001 ctgtattttc aaggcttgtg tattcaatga tattagacac tgaaaacctc ctgtatataa
     3061 aagcagcaaa aagctatgga agctcaaaag gccaaatatt tttcagacat tacatccctg
     3121 caatgcttcc aagactgatc acccaattca gctcatccat aggctcagcg gttatgattg
     3181 aaacgtccct gtcttttttg ggacttggaa ttcagccgcc ctttgccagc tggggattga
     3241 tgctcagtga atcaagacag tttttcatgg gttaccccta tttggcggtt gcaccgggga
     3301 ttgcaataat aataaccgtc ctgggattta accttattgg agacgggttg aacgatattc
     3361 tgattgacag ggagggcagc gatgaataag actgttgagc tgaagaacct ccatataaaa
     3421 gccggtgata atacattggt aaataaaacc tctcttacta tagaggaagg agaaattttc
     3481 gggctggtgg gcgaatcagg ctcaggaaaa actcttacgg caaaagcaat tatagattta
     3541 ctttctgaag gattaagtta tagagccgat gaaataataa ctgccgggaa gaatatgctt
     3601 actgcctcaa gggaagaaaa aagagagcac ataggcaaaa atatcggata cattccacaa
     3661 aatactgtgt tttatctgca tcctatgatt aagataaaaa atcaaatcgg agacggatat
     3721 attcatcaca tgaataaaac caggaaggaa gcattggaaa aagcagaaac cttgttaagc
     3781 aaggttggct ttaaatctcc caggaccgtg ctgaattact atccatggca gctgagcgga
     3841 gggatgcgtc aaagatgcaa tattgccatg gccttgatga atgaccctaa gcttattatt
     3901 gctgatgagc ctacgactgc tttggacacc actgttcaaa gacaggttat ggagctcttc
     3961 agaacaataa atgaagaaat gaagctgtca atacttttga tttctcacga tttgggactg
     4021 gtaaagaatt acagccacag cctgggagta atgtatgcag gtcagattgt tgaatcagga
     4081 tatacaaata cagtattcag caatccaaga catccatata cccggctgtt gataaaaatt
     4141 atcccttccc tgaaaatcaa aaagagccag cccttgactg aaatcccggg atttgtaaag
     4201 gataagggaa gagatggcga tggatgtttg ttttacaaca ggtgtcccca ggctatggat
     4261 atatgtaaaa agtatgcggc ggagcatata gatgataaaa tggaccacta ctacaggtgt
     4321 aatttggaat agaggctgtg ttatggcaaa taaagttgta atttctgcag tgaatataag
     4381 aaaagaatat gtgacaaaaa agcattttct tacaggcaaa accaaagaaa aatatgttgc
     4441 cgtaaaaggt gctgccttta atattgaaga aggaaaaaca cttggaattg taggggagtc
     4501 gggaagcggg aaatccacaa taggcgaaat agtgggaggg cttcaaaaac ctacatacgg
     4561 caatgttctt tatttcggca aaaatattaa tgaactgtca aaggaagaat acctggatta
     4621 caggaaaagt gtacagttta tttttcaaga ccccaagggt tctatggacc acaactataa
     4681 aattttcgat attttatcca tgcctttatt gactgcgaaa attgcaagag ataaaaatga
     4741 agctgaaaaa ttaattgatg aaatgcttat gagagcaggc cttgatttaa gcataaagaa
     4801 aaaatatccg actgaaataa gcggaggcca gtgccagagg gcagcaatag caagagccct
     4861 gcttgttaat cctaaactta taatctgtga tgagcctgtt tctgcccttg atgtatctgt
     4921 tcaggcccag atacttaact tgctcagaga gcttcaaaga cagctgaata tttcatatat
     4981 ttttatatct cacgatatcg gagtagtaaa ctatatgtcg gatacaattt tagttatgca
     5041 gaacgggact attgttgaaa gaggggaagc aaaggatgta ctcctgcgcc cgaaagaagc
     5101 ctacactaaa aaactcatag aaagcgcatt tatgaactag agatgtccta accccatttc
     5161 tcagaacctg atttggggac attcctctta ccaacagaga ctatccctaa atagggaggt
     5221 gtgtccccat acctctaata gaaatgggtg gcaggtcatt cgcaacgagt tcagaattta
     5281 tgcgaccgca gggagcaaat aaattagtga atttccctgc attatgtgac tagggaagac
     5341 tgtaaatata aaaagttaat atgaaaggtt atataatgga agttttactt aaagaaaatg
     5401 aaaaaataga agacttgcaa tgtaaaggat taaaaatcat tcaaaataaa aaatggtttt
     5461 gttttggcat ggacgctgtg cttctgacaa attactgcga tatacgaaag aattcaaaaa
     5521 ttgtcgattt aggcacaggc accggaataa taccgatact cttaagcggg aaaaggaact
     5581 attccaaggc ttacgggctt gaaatccagg ctgaaattgc agaaatggct caaagaagcg
     5641 ttaaattgaa ttctcttcag gaaaaaatag aaattttaaa catcgacttg aaagatgcat
     5701 tggaatatct tgacgctgat tcatttgacg ctgttatttc caatccgccc tacaagcttt
     5761 ataacagcgg tataattaat cccgatgaca aaaaagcaat atcaagacat gaaataaagt
     5821 gcagcttgga ggatgttata tcaacagcat caaaattgtt aaagcagtac ggcaggtttt
     5881 acatggttca tcgtcctgac aggctggctg atataatgtg tcttcttaga aagcacaggc
     5941 tggagcccaa gcaaatccgc tttgttcatc caaaggttaa agcaaaacca aatatgattt
     6001 taatccgtgc atctaaaaac ggcaatccgg aattaaaatt cgaccctcca ctttatattt
     6061 atgatgaaga gggaaattat acagatgatg tttaccagat ttacgggatg gtgaacagct
     6121 gacattattt gtcaagatta ttattaattt ctgctttgag gatacagtat tcaacacact
     6181 tttaaatatc cctctcagat tttgcgatgc ttcctttctg ccaatctata tgaataaaat
     6241 gctcaaagtt tcaattaatg tgttatttgt ctgtatttat atacacatta cttgaaattt
     6301 cgagcatcca aagactgttt atctcatttt acgactaaat ggaactaaaa ggtatttcct
     6361 tttatttttt tattctgatt ttatctatcg gaagcactgc aggaccttcc agaatatgtc
     6421 cattaatatc atatcttgag ccgtggcagg gacaatccca tgttttctcg gcttcattga
     6481 atgaaagcgc acatttcagg tgggagcata ccggattgat gcaatagtat tctcctgctt
     6541 catttttgta cactcctact tttttaccgt catagtcgac tattttccct tctcccggct
     6601 tgacatccga aaattcttcc aagtcacctg ctaatctgtt caaaaaccct aaagcaatac
     6661 ttttaaccga agatacgaat tcttttgctg attggacagg agtgaatctt gaaggattga
     6721 aaatacctga ataatcatct tcaatgcaaa gaattttgtt tcttaaaatt aatgcagatg
     6781 ctgcagaatg cgacattccc cattttttaa atccggtagc cacatataga ttgtctatgc
     6841 tgtccgaata ccttccaatg tatggaattt tatcataggt catgcagtcc tgggttgacc
     6901 atttcgaaac tatttcagga tttttaaaat tgctgttaag aaacctttcc aattcattat
     6961 agcattcact ttcatcatcc ttttcaccgg acctgtgatt gcctcctgca agaattaaaa
     7021 gattttcatt atcagaaaag tggtacctca tggaataaat agggtcttta atgtttatgt
     7081 acattcctcc gaatggctgc tcctttgttc ttgcagcaat aacatatgat ttttcctgat
     7141 atagtcttaa aaaataaaga ccgaaactgt tgtcaaaggg ataatgagac gcagctatta
     7201 ccttgcctgc atgaatggtt cctttttcag ttttaacatc tacgtgatca tcgtgaggat
     7261 taatatccaa agctcttaca ttctcatata tagcaatatt ggcggtactt aaaatattat
     7321 aaaggctgta taaatatttt aatgggttga actggccctg gttttttacc cctattgcac
     7381 cgtaagcctt gttcggcaga ggcaccgtat ccgtatagaa cccgtttatg cctaattttt
     7441 cataagcttt taactctttt ttcaaatcat caacttcatc aatggagtat gcgtagacat
     7501 atgcatcctg gtctttgtaa tcacattgaa tattgttttc gactattatt tttttaatta
     7561 attttactcc ttcttcatta gctttcaaat attgctttgc ttcttcaaaa ctaaagttgt
     7621 ttattaaatt gtcataaaac aaatcgtgct gaattgttat ttttgccgta gtaaaacctg
     7681 ttacaccgta accggcactt gttgcttcga taattcctat gttcaaacct aaatccttta
     7741 ataaataagc agttgtaagt cctgtaatcc ctgctccaat tattaaaatg tcaaattcct
     7801 tgttgtttaa atccacattt aaactggcat ttttcacctg gggatttttt atgttttcag
     7861 ctgtatcgtg ccagtatgaa tgtctcgggt attccatttt tctacctctt ttcttattat
     7921 tttctttaat ttgcatatat tgttcacagc aattaagaag aataataaaa gtttattatt
     7981 tttccatgat gtaatgttta ttgtctcata aatatttcaa tttattcttg actttatcga
     8041 aaaattcata tatcataaaa cagaattaaa acaataaaga cagtgattgg aacagtaatt
     8101 gcacgcagga ttcaaagaga tataattgtg tggtgaaaga ttataagtcc gaatgcaacg
     8161 aagatcatcc aggagtttaa tttctgaaag aaccagtaag aaattacgta caaatgcgtt
     8221 aaatttttga gtgacatata tataatatat gtaatttggg tggtaccgcg gaattcgtcc
     8281 catgtcctta gacatgggac tttttgatta cctatacctg ttttttcaag ctatagtgca
     8341 gaataagcag cggaaggtca gatgcagcac aatcccaata tgcacgaccg atggaagtga
     8401 ataaattggt gatggagcct gccataaggg atgcattaat aaaaatataa gaatttataa
     8461 tttcaacaat taacataggt ataggaggaa aaaatgaaat tattcaaaga tatatcaaaa
     8521 gctgattata gaaataatga gcatgaaata tcaaaatatt gggaagaaat tgacttattg
     8581 gagcaaagca ttgaaacaag gaaaaacaaa ccatcctttg ttttttatga aggtcccccc
     8641 actgcaaatg gaaaaccggg aatacatcac gttactgcaa gaacattgaa agactctgta
     8701 tgcagataca aaaccatgaa aggttaccag gtaaaaagaa aagcagggtg ggatactcac
     8761 ggacttcctg ttgaaattga agttgaaaaa caattaggct tatcaacaaa gcaggaaata
     8821 gaagagtacg gtattgccga attcaataaa aaatgccgcg aatcggtgtt cacctatgaa
     8881 gcacactggc gtgaaatgac cgaacgtatg gcctttttaa ttgacatgga tgacccctat
     8941 attacacttg aaaatgacta tattgaatct gaatggtgga tacttgataa agttttcaag
     9001 gacggcctga tatatgaagg ttataaaata cttccatact gccccagatg cggaacaggt
     9061 cttgcttctc atgaggttgc acaaggttat gaagaaataa agaccatgag tgcttatgtg
     9121 gcatttaaga ggaaggacgc tgatgagtat tttcttgcat ggaccacaac accatggaca
     9181 ttattgtcaa atgtatcatt gacagtaaat gcaaatatgg attatgtaaa aatcagaaca
     9241 aaggataaag aagatgtcaa gggcaaggtt tatattcttg ccaaagattc ggcaccaagg
     9301 gttatcaatg aagaatatga agtgcttgag gttaagaccg gcaaggaatt ggaattcatg
     9361 gaatatgaac agctcatgcc ctttgttgag gtgccggagg gcaaaaaatc attctatgtg
     9421 acacttgcag actatgtgac tattgaagat ggaacaggta ttgtccacac cgctcctgct
     9481 tttggcgagg atgattacaa cacggggatg ctatacggcc tgccggtttt aaatcctgtt
     9541 gatgatacag gaagattcag gggtacacct tggtccgaca tgtttgtaat tgatgcagac
     9601 cagcccatat taaaatggct taatgaaaat ggagtattat acaaaaagga aatttttgct
     9661 cataattacc cccactgctg gagatgccat acacctttat tgtattatgc aagaccaagc
     9721 tggtacattc aaatgactaa gcttaaggat ctgcttgttt ccaacaacaa tactgttaat
     9781 tggtttccgg attatgttgg agaaaagcgt tttggaaact ggcttgaaaa tgttaatgac
     9841 tgggctattt caagaagccg ttactggggg acgcctcttc ctatctggag atgtgaatgc
     9901 ggtcatttag aaagtatagg ttcaagaaaa gaattagttg aaaaagcagt tgaagaaata
     9961 gatgaatcaa ttgaacttca cagaccatat gtggatgaag tgcatattgt ttgcccaaaa
    10021 tgcggcaagc acatgaccag agtaaaggat gttattgact gctggtttga ttcaggtgct
    10081 atgccttttg cacagtggca ctatccattt gagaataaag aaagatttgg cgaattgttc
    10141 cctgctgaat ttattaatga aggcattgac cagacaagag gatggttcta ttcattaata
    10201 gccgtatcca ccctggtaac gggtaagacg ccatatagaa atgtattggt taacgacctg
    10261 gttcttgata aatttggtaa gaaaatgtca aaatcaagag gaaatactgt agagcccatg
    10321 gacctgttcg atagatacag cgttgacgca ttgaggtggt atcttctgta tgtgtcccct
    10381 gtatggacac ccacccgttt tgacgaagac ggtttaaagg aaatattgag caaatttata
    10441 gggacgatta aaaacgtata taccttcttt gccttatatg caaacaccga ttccatcaaa
    10501 ccccaggact actttgttga atacagggat aggcctgagc ttgacagatg gattgtatca
    10561 aaatataaca acctggtgaa atatgttact gaaagcatgg atgtttatga ccataacaaa
    10621 tcagtaagag caattcaaaa ttttgtcaat gaagatttgt caaactggta tatcaggcgt
    10681 tcgagaagaa gattctggga gacagagctt actgaagaca aaaaggcggt ttatgtgacc
    10741 acatatgaaa tattggttgg aatttctcaa ttaatagctc catttgcacc ttatttgtca
    10801 gaagaaattt acagaaatct aaccggccag ccatcagtcc acttaagcta ttttcctgaa
    10861 gcaaaggagg agctgataga tgaaaatatt gaaaagagaa tggatttggt aaaagacata
    10921 gtaagcttgg gaagagcatc aagagagaat gctaaaatca aggttagaca gcccattcaa
    10981 aaaattcatg tagacggcaa atacaagcaa ttaattgatg atttggtccc cctgataaag
    11041 gaagaattaa atgttaaaga agttgtgttt gagcatgact taaatgaatt catggatttc
    11101 tcgctaaaac ccaacttcaa gattgcagga cctatgctag ggaataatgt taaggcattc
    11161 gcaggaatcc tggcaaaagc tgacgcattg aacataataa caaaggttca atcaggtgaa
    11221 aaatgtgaat tggatgttaa cggacaaatc gttgagcttg acaatcaatt gctggatata
    11281 aggatttcag caaaggaagg ttatgatgtt gcaacaggaa acaatttatt tacgataata
    11341 gacaccaacc ttactgaaga gcttctgaat gaaggctatg cccgtgaatt catatccaag
    11401 gtacagcaga tgagaaagaa caacggatat gagatgatgg acagaataga aatatactac
    11461 acaagctcac ctgaaatcga taaggctgta aatgattatg aagattttat caaagaagaa
    11521 actttagcag acttaatttc tgtatcagaa gaagatatgg aagttcaaaa cctgaacggt
    11581 gttgatacag gaatgaagct tgaaagaata agataatgcc ttcacatatg tgaaaatgca
    11641 agctttataa tataactaac ggtatag
//
LOCUS       DAOPUT010000049        11248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_5866_length_11248_cov_17.698830, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000049 DAOPUT010000000
VERSION     DAOPUT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5866_length_11248_cov_17.698830"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..657
                     /locus_tag="PKK75_05515"
     CDS             235..657
                     /locus_tag="PKK75_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNZ83091.1"
                     /translation="MKKFNVTVNGKTYAVEVEEVGAGQGGFAYQPVKQAPVQQPLQQV
                     VQSAPAAAPVQPAPAAVPKAAPVDGDGELMSAPMPGTILDILVTEGQAIKKGDIVLIL
                     EAMKMENEIASPADGTVKRIHTSKSETVSAGDPLLTIA"
     gene            745..2424
                     /locus_tag="PKK75_05520"
     CDS             745..2424
                     /locus_tag="PKK75_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNZ83092.1"
                     /translation="MFDKEKLSHLKEEREKWEKGVLGKTLSKAPESKDEFKTVSGSVV
                     NRLYTPEDVANIDYESEIGYPGQYPFTRGYQPTMYRGRPWTMRMYSGFATAEESNERY
                     KYLVSQGSTGLSVAFDLPTQIGYDSDHTLAQGEVGKVGVAIDSLKDMEILFDGIPLDK
                     VSTSMTINAPAAVLLAMYIKVAEKQGVSPNKLRGTIQNDILKEYIARGTYIFPTEPSM
                     RLITNIFEYCSKEVPNWNTISISGYHIREAGSTAAQEVGFTLADGIAYVDAAIKAGLD
                     VDTFAPRLSFFFNSHNDLFEEVAKFRAARRLWAKIMKERFKAEDEKSMMLKFHTQTAG
                     CTLTAQQPNNNIIRVAMQALAAVLGGTQSLHTNSRDEALALPTTESVTIALRTQQIIA
                     SETGVTNTVDPLAGSYFIEAKTKELEEKAMEYIAKIDELGGAARAIDLGYIQKEISDA
                     AYKYQMEIESKETIVVGVNKYKVEEEAPKGLLRVDPIVAEMQKKKIDDVKAQRNNAAV
                     EEKLQLLKKACEGTENVMPLILDAVGEYATLGEICGVMREVFGEYQQAVLI"
     gene            2435..2830
                     /locus_tag="PKK75_05525"
     CDS             2435..2830
                     /locus_tag="PKK75_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNZ83093.1"
                     /translation="MNRPIRVLIAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTP
                     EQIVAAAVQEDVDVVGMSLLSGAHNYLFPRVAQLLKEEGVDDVLVIGGGIIPDEDIQG
                     LKEAGIAEIFTSGTPTSRMIEYIKNNLKR"
     gene            2999..3940
                     /gene="meaB"
                     /locus_tag="PKK75_05530"
     CDS             2999..3940
                     /gene="meaB"
                     /locus_tag="PKK75_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNZ83094.1"
                     /translation="MNYSQLIEKMLAGDKRACAKLITAVENDPDKAEKIIREIHQYTG
                     KAHIIGITGPPGAGKSTLTDKIVKALVSEGNKVGVIAIDPTSPYSGGSILGDRIRMQE
                     LALNPDVFIRSMGTRGYLGGLSKQTKNALKILDAAGYNYIIIETVGVGQSEVDIVKVA
                     DTIVMVLVPGLGDDIQSIKAGIMEIGDIFAVNKSDLFGADRTAVEVNMMLDLTMDMDR
                     RPPVVKVTAVKNEGIDDLVAEIKAHMNYLKESGKFEERRKNNLKLEVVELIEEELRKI
                     VISLTDSENRLDGQVIEILNKNKNIYQVRDEFLKALN"
     gene            3977..4378
                     /gene="mce"
                     /locus_tag="PKK75_05535"
     CDS             3977..4378
                     /gene="mce"
                     /locus_tag="PKK75_05535"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNZ83095.1"
                     /translation="MVKKIDHIGIAVKNLNETLNFYENILGLKSAGTETVEEQKVKVA
                     FLPIGDTEVELLESTEEDGPIARYIAKNGEGVQHIAYKVDDIEKAIDEMKNKGIRMID
                     EKPRYGAGGAKIAFCHPKSTYGVLIELCQRD"
     gene            complement(4510..5250)
                     /locus_tag="PKK75_05540"
     CDS             complement(4510..5250)
                     /locus_tag="PKK75_05540"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ83096.1"
                     /translation="MEKLTTENITVSYEGSKTIEDISVKLNKGEIVSILGVSGVGKTT
                     LFNVVSGLIKPDSGAVLLDGKDITNTTGNTSYMLQKDLLLPYKTIIDNVSLPLVIRGE
                     KKAKAREYASKYFDEFGLMGTENKYPHQLSGGMRQRAALLRTYLFSDQVALLDEPFSA
                     LDSITKSSMHRWFLKVMEQIKLSSFLITHDIDEAILLSDRIYIMSGKPGRITDEIIID
                     EPKPRDKDFIVSENFMKYKKRILDELDS"
     gene            complement(5269..>6107)
                     /locus_tag="PKK75_05545"
     CDS             complement(5269..>6107)
                     /locus_tag="PKK75_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ83097.1"
                     /translation="QAKGFFEEEGIEAVIMQPPEGGAEAIVGGGGAEFGIGFQDYLAP
                     NYASKTPMPITAVAAIIQHNTSGIISLKEDGIDTPAKLAGHNYATWNLPIELAIIEKI
                     VGDDGGSFKDIDLVPSTVTDVITALQTDIDSVWIYYAWDGIATKVKGLETNFLNFADY
                     GRELDYYSPVLFVNDNFAEANPEVVKKVLSAVKRGYEYSIEAPEAAAQILLGAAPELD
                     PEIVVESQKWLADQYIADAPKWGYIDQERWDGFYGWLFENNLIEMEIPKGRGFTNEYL
                     PD"
     assembly_gap    6108..6207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6208..6428)
                     /locus_tag="PKK75_05550"
     CDS             complement(<6208..6428)
                     /locus_tag="PKK75_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018580042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ83098.1"
                     /translation="MKNKVMFLTSVLIISLFLMTSCGSETPGPQPGEDTVKVDIVLDW
                     TPNTNHTGLYVAQAKGFFEEEGIEAVIMQP"
     gene            complement(6442..7209)
                     /locus_tag="PKK75_05555"
     CDS             complement(6442..7209)
                     /locus_tag="PKK75_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83099.1"
                     /translation="MKKKFRNIIDNASPVLAIVLILAAWQFLSSTGLVPKFMLPSPYD
                     VVTAFINDFFLLMEHAKTTLAEALLGLTVGTALGFAIAVLMDRFSLFEKSAYPLIILT
                     QTVPTVAIAPLLVLWLGYGLLPKVTLIVLTTFFPITVSLLEGFHSADPDALNLLKAMG
                     GNRFQTFMHIKLPSALNQFFSGLKISVSYSVVGAVIAEWLGGLSGLGVYMTRVRKSYS
                     FDKMFAVIFFISIISLLLMFAVSYLKKAVMPWENKKN"
     gene            complement(7181..7474)
                     /locus_tag="PKK75_05560"
     CDS             complement(7181..7474)
                     /locus_tag="PKK75_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HNZ83100.1"
                     /translation="MEANIAIQVLPHVSDDEEVLRIVDEVIDYIKGTGLNYYVGPSET
                     SVEGDYDQLMEIVKNCQLVAIRAGAPKVSAYVKISLAPEGGVFTIEEKVSKYH"
     regulatory      complement(7552..7683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(7810..8292)
                     /locus_tag="PKK75_05565"
     CDS             complement(7810..8292)
                     /locus_tag="PKK75_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83101.1"
                     /translation="MKKIIIFTLILALAFTAVIYAAPGDSNDPIVVLSYLNDRIKALV
                     SDYKLDELENLKKKVDDLTVGGTGGSSAALQIVEIRAGERLVAGAGTELILRGGKAFV
                     IGSELGGISDVTVGKDFVSGMEFVSNHLMIVPRGDGRGAYTNDYAIFMVRGTYEIIKQ
                     "
     gene            8520..9362
                     /locus_tag="PKK75_05570"
     CDS             8520..9362
                     /locus_tag="PKK75_05570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ83102.1"
                     /translation="MYKLCMTVNKLFKLPVHPFNLHNEGKKTYAQWQYEKGMDTIKFY
                     LKHSTVDEMFKDKIVLDIGCGAGGKTVFYASKGVKKITGIEILGKYKEEAENLAKEFN
                     LQDKFEFVQGDAANMPFENEAFDTIIMNDAMEHVDKPEMVLGECYRILKKDGKLYLNF
                     PPYNHPYGAHLSDAIGIPWVHVFFSEKTLIKTYKDLVKDLPDGKERIDFRISKKEDGT
                     EYFSYINKMSIKRANTILHNSSFELEYYSEEPLRSFFKYTARIPFLKEFLVKMVVCVL
                     RKMP"
     gene            9624..>11248
                     /locus_tag="PKK75_05575"
     CDS             9624..>11248
                     /locus_tag="PKK75_05575"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HNZ83103.1"
                     /translation="MKLKVDKIVLILICLSLIVGLYTLYQRVEIEKQYKTTEIVLDYN
                     EMKKFADSSNNDLSYWMRKFREFGAKSVAIQEETIKLLIEAGYSLRAQMVSELIKEYK
                     WQEDYNREIVRAISENEISPFDVIVTTEDEEVYSYILSGLEERYADDFYQTHIYDNVY
                     YIVLKGIVDDVYYTELERTVNLAGKGVYEVQKAVDSRLMNIGIGYDSEKITMAKDAGL
                     DVILRPINFPASNEKLADAYKAANEKYGLEPRIYLVHGKEVLGYPNNEDKLLNYIKEE
                     NIAVALIESGTQRQHLEQEGLDELVEDSGYQALRAFTMWDFIRERNRYYNYEGAEEIE
                     NTMFRAVTERNIRVIYFKPFFEEKDSTKFLTDVDEYERTFKSLENRLSAHNISFGKAK
                     AMEYFTIGSKRMAVLAFGITLAAVMLFIKMFNIKHKFADYLYLFALPAALIPLVMRKL
                     AEKGFAFGSAVVFSGLAIYFFMIQIKRIYESEEKYSNLQIIVYSALILAGSVAIALAG
                     AVFVVSMLADVKYMLEMDIFRGVKFAQILPFGI"
BASE COUNT         3641 a         2119 c         2365 g         3023 t
ORIGIN      
        1 cccctgaaat ggactcaata tgggcacatg cagggagaat gaaattgatg agataaagat
       61 gccctaaccc tatttttcag agtctttttt ctgaaaaata gcggtaggtc attcgcaacg
      121 agcgccagtt ttgtgcgacc aacgggagca tacaaaaatg tgcagcgaga ctgcgcatat
      181 ctattatata gagaaaatct aatttaaata ataatatacg gaggttaatc taaaatgaaa
      241 aaattcaacg taacagtaaa cggcaagaca tatgcagtag aagtagaaga agtaggagca
      301 ggccagggag gatttgcata ccagcctgta aaacaggcac cggtacagca gccgttacag
      361 caggtggttc agtcggcacc tgcagcagct ccggtacagc cggcaccggc agcagtaccg
      421 aaagcagcac ctgtggacgg tgatggagaa ttaatgtcag ctcctatgcc gggtacaatt
      481 ttggatattc tggtaacaga gggacaggca attaaaaaag gtgatatagt attgatactg
      541 gaagctatga aaatggaaaa cgaaatagca tcaccggcag acggtactgt taaaaggatc
      601 catacttcca agtctgaaac cgtaagtgca ggagaccctc tgctgactat tgcataaaca
      661 attacttaat aaaaagtgca gcatttcatt catagtgtat ttcactaagg atgagaaaac
      721 ctgcgaataa tattaggagg aaaaatgttt gataaagaaa aactttctca tttaaaagaa
      781 gaaagagaaa aatgggaaaa aggcgtttta ggaaaaaccc taagcaaggc gcctgaaagc
      841 aaggatgagt ttaaaaccgt ttccgggtct gtagtcaaca gattatacac tcctgaagat
      901 gttgccaata tcgattatga gagtgaaata ggatatccgg gacagtatcc attcacaaga
      961 ggctaccagc ctaccatgta caggggaaga ccatggacta tgcgtatgta ctcaggattt
     1021 gccaccgcag aggaatcaaa tgaaagatac aaatacctgg tcagccaggg gtctacggga
     1081 ctatcagtag catttgacct cccaacccag attggctatg attcagacca tacactggca
     1141 cagggggagg tcggcaaggt aggtgttgcc attgactcgc ttaaagatat ggaaatattg
     1201 tttgacggaa tacctcttga caaggtaagt acatcaatga ccataaatgc cccggcagca
     1261 gtacttttgg caatgtatat caaagtcgcg gaaaaacaag gtgtatctcc gaataaatta
     1321 agaggcacaa ttcaaaatga catattaaag gaatacatag caagggggac atacatcttc
     1381 cccacagagc cgtctatgag gcttattact aatatattcg aatattgctc aaaggaagtt
     1441 ccaaattgga atacaataag catttcagga taccacataa gggaggcagg ctcgactgca
     1501 gcccaggagg taggatttac actggcggac ggaattgcat atgttgatgc cgcaattaag
     1561 gcaggtcttg atgttgatac atttgctccc aggctttctt tcttctttaa ttcacataat
     1621 gacctgtttg aggaggtagc caaattcaga gctgccagaa gactctgggc aaaaataatg
     1681 aaggaaagat tcaaagctga ggatgaaaaa tccatgatgc tgaaattcca cactcaaact
     1741 gccggctgca cacttactgc ccagcagcca aacaacaata ttataagggt tgcgatgcag
     1801 gctttggcgg cagtgcttgg aggaactcag tccctgcata ccaattcaag ggatgaagca
     1861 cttgcccttc ccacaactga atcggtaaca atagctctta gaacccagca gattattgca
     1921 agcgaaacag gagttacaaa tactgtagac ccgctggcag gctcatattt cattgaagca
     1981 aagactaagg agctggaaga aaaggcaatg gaatacatag ctaagattga tgaactgggg
     2041 ggagctgcaa gagccataga tttaggatac attcaaaagg aaatttcaga cgctgcatat
     2101 aaatatcaga tggaaattga atccaaggag acaatagttg ttggagtcaa caaatataag
     2161 gtggaagaag aagcaccaaa aggacttttg cgggttgacc ccattgttgc ggaaatgcag
     2221 aaaaagaaaa ttgatgatgt taaagcacag agaaataatg ctgctgttga agaaaaactg
     2281 cagctgctta aaaaggcctg tgaaggaact gaaaatgtaa tgccccttat acttgatgca
     2341 gtcggagaat atgctacatt aggagaaata tgcggcgtca tgagagaagt tttcggtgaa
     2401 taccagcagg cagtgttgat ttaggaggga tatgatgaac agaccaatta gagttttaat
     2461 tgcaaagccc ggtcttgacg gccatgacag aggcgccaag gttatagcga gagcccttag
     2521 agatgcaggc atggaagtaa tttatacagg gcttagacag actcctgaac aaatagttgc
     2581 agcggcagtg caggaagatg ttgatgttgt ggggatgagc ttattgtcgg gagcacacaa
     2641 ttatttattc cccagggttg cccagctatt aaaggaagaa ggcgtcgatg acgtgctggt
     2701 aataggcggc ggaataatac ccgacgagga tatccaaggc cttaaggaag cagggatagc
     2761 agaaatattc acatcaggca ctccgacaag caggatgatt gagtacataa aaaacaattt
     2821 gaaaagatga tgccctttat gacctaaccc aatttctacg actgaaaaga agaaagaaat
     2881 tgatggtagg tcaaacgcat aaaattccta atttaagcga cgattaggag caaataaatt
     2941 agtgaattta cctgcgaacc atatttttca aaggataatg gagtttagga aggtcggaat
     3001 gaattattcg caattgatag aaaaaatgct tgcgggcgac aaaagagctt gtgccaagct
     3061 tataactgca gtggaaaatg atcctgacaa ggctgaaaaa atcataagag aaatacatca
     3121 atatacagga aaggcccata taatcggaat aacaggtcct cccggtgccg gaaaaagcac
     3181 ccttactgat aaaatagtca aggcactggt gagcgaagga aataaggtgg gagtaattgc
     3241 aatagaccct accagtcctt attcaggtgg ctcaatcctg ggagacagga tccgcatgca
     3301 ggaattggca ctgaatcctg atgttttcat acgcagcatg gggacaaggg gttacctggg
     3361 aggtttgtca aagcaaacaa agaatgcctt aaagattctt gatgcagccg gctataacta
     3421 tataatcatt gaaaccgtag gggtaggcca atccgaagtt gatattgtaa aggttgccga
     3481 caccattgta atggttttgg tgcccggact tggagatgac atacagtcaa tcaaggcagg
     3541 cataatggaa ataggcgata tttttgctgt caataaaagt gatttgtttg gagctgaccg
     3601 aacagctgtt gaggtaaaca tgatgcttga tttgaccatg gatatggaca gaagacctcc
     3661 cgttgtaaag gtcacagctg ttaaaaacga aggtatcgat gatcttgtgg ctgaaatcaa
     3721 agctcatatg aattacctca aagaaagcgg taaatttgaa gaaagacgaa aaaacaacct
     3781 taagttagag gttgtagagc tcatagagga agagcttaga aaaattgtca tctccctcac
     3841 agacagcgaa aacagattag atggccaggt aattgaaata ctaaataaga ataaaaacat
     3901 ttaccaggta agagatgaat ttttaaaagc tttgaattaa atatttaaaa atgtccggat
     3961 gaattggagt gatattatgg ttaaaaaaat agaccacata ggtattgcag tgaaaaattt
     4021 gaatgaaacg ttaaattttt atgaaaatat tcttggctta aaatcagcag gaactgaaac
     4081 tgtagaggaa caaaaagtta aggttgcttt tcttcccatt ggagacacag aggtagagct
     4141 tttggaatca acagaagaag acggacccat tgcaagatat attgccaaaa atggcgaagg
     4201 ggtccagcat atagcataca aggttgatga catagaaaaa gccatagatg aaatgaaaaa
     4261 caaaggtata agaatgatag atgagaaacc ccgctacgga gccggaggtg ctaagatagc
     4321 attttgtcat cctaaatcca cttatggagt tcttatagaa ctgtgtcaga gggattaaag
     4381 aggaaaaaat agaagaatca ataaactaaa gctgcggatt atccgcagct tcgattttac
     4441 aatgataaaa actatctttt ttgccggctt aattattaag gttactttta cctgccgtct
     4501 tttaatattt taactatcca attcatccaa tatgcgtttc ttatatttca taaagttttc
     4561 gcttacaata aaatccttgt ctcttggttt tggctcatca ataattattt catcagttat
     4621 tctgccgggc ttgcctgaca ttatataaat tctgtcagac aatagaatag cttcatcgat
     4681 atcatgggtt atcaaaaagc tcgaaagctt aatctgttcc atcaccttaa gaaaccacct
     4741 gtgcatgctg cttttggtta tactgtccaa ggcgctgaag ggctcatcca aaagtgccac
     4801 ctggtcggaa aacaaatatg tgcgaagcaa ggcagccctc tgcctcatgc ctcctgacag
     4861 ctggtggggg tacttgtttt cagttcccat aaggccgaat tcatcaaaat attttgaagc
     4921 gtactctctt gcttttgcct ttttctctcc ccttatgacc agaggcaaag aaacattgtc
     4981 aattattgtc ttgtaaggaa gaagcaggtc tttttggagc atgtagcttg tatttcctgt
     5041 agtgttggta atatctttgc catccaataa aacggctccc gaatcaggtt ttatcaaacc
     5101 tgatactacg ttgaacaagg tggttttgcc cactccgctt acgcctaata tgctcacaat
     5161 ctcaccttta tttaatttta cagaaatatc ttcaattgtt ttgctgcctt cataagagac
     5221 agttatgttt tcagttgtaa gtttttccat acttccacct aaactgtcct aatcaggaag
     5281 gtattcattg gtaaagcctc ttccctttgg aatttccatt tcaataagat tgttttcaaa
     5341 taaccagcca tagaagccgt cccatctctc ctggtctata tatccccatt ttggagcatc
     5401 tgcaatatat tggtccgcca gccacttttg actttccaca actatttcag ggtcaagctc
     5461 gggggctgct cccaagagta tttgagctgc cgcttccggt gcttcaattg aatactcata
     5521 gcctcttttg acagctgaca gtaccttttt tacaacttcc ggattagcct ctgcaaaatt
     5581 atcattaaca aacaaaacag gactgtaata atccaattcc ctgccgtagt ctgcaaaatt
     5641 caaaaaattc gtttccagac cttttacttt tgttgcaatc ccgtcccagg cgtaataaat
     5701 ccacacagaa tcaatatctg tctgcagggc tgtaatgacg tctgttacag tgcttggaac
     5761 caggtctata tctttaaagc tgccgccgtc atctccaact atcttttcaa ttattgctaa
     5821 ttctatgggc aggttccatg tggcatagtt atgtcctgcc aattttgccg gagtgtcgat
     5881 gccgtcctcc tttaaggata ttatgcctga agtattatgc tgaattattg cagcaaccgc
     5941 agttataggc attggtgttt tagaagcata atttggagcc agatagtcct ggaagccgat
     6001 tccaaattca gcgcctccgc ctccaactat agcttcggct cccccttcag gcggctgcat
     6061 gattacagct tcaatccctt cttcttcaaa gaagccctta gcctgggnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnggc tgcatgatta cagcttcaat cccttcttct
     6241 tcaaagaagc ccttagcctg ggcaacgtac aagccggtat gatttgtatt cggagtccaa
     6301 tccagtacta tgtccacttt tacggtatct tctcccggct gtggacccgg tgtttcgctc
     6361 ccgcatgatg tcatcaaaaa aagcgatatt attaatactg atgttaaaaa cattacttta
     6421 ttcttcatga aatgcgtcct ttcaattttt cttgttttcc caaggcatta cagccttttt
     6481 taagtatgat actgcaaaca taagcagcag gctaatgatt gatatgaaaa atataacagc
     6541 aaacatttta tcaaaggagt aagattttct tacccttgtc atatatactc caagtcctga
     6601 caatcctccc agccattcgg caataacagc acctaccacc gagtaggaca cagagatttt
     6661 aagtcccgaa aaaaactggt ttaaagcact tggcagcttg atatgcataa aagtctgaaa
     6721 tctgtttccc cccattgcct ttaacaaatt taaggcatca gggtctgccg aatgaaatcc
     6781 ttcaagcagg cttacggtta tggggaaaaa agttgtaagt acaatcaatg ttaccttggg
     6841 cagcaggccg tatcccagcc acaaaaccag aagaggggct atggccactg tcggtaccgt
     6901 ctgtgtaagt atgatcaggg ggtatgctga tttttcaaac aaggaaaatc tgtccatcag
     6961 tacagctatt gcaaatccaa gagccgtacc tacagtcagt cccagtaatg cttctgcaag
     7021 tgtggttttt gcatgctcca tcaataagaa gaagtcgttt ataaaagccg taactacatc
     7081 atatggagag ggcagcataa atttcgggac aagacccgta gacgataaaa actgccaggc
     7141 ggccagtatt aaaactatag ctaatacagg ggacgcatta tcaatgatat ttcgaaactt
     7201 tttcttcaat ggtaaacacc ccgccctcgg gagccaaact gatttttaca taggcagaca
     7261 ccttgggggc ccctgctctt atggcaacta actggcagtt ttttacgatt tccatcaatt
     7321 ggtcgtaatc accttcaacg gatgtttcgc tgggtcccac ataataattc aatcctgttc
     7381 ctttaatata atcaattact tcgtcaacta tccttaaaac ctcttcatca tctgatacat
     7441 ggggtaaaac ctgtatcgca atatttgctt ccatttaaac ctcctgttag tattatttaa
     7501 taacaaaaac ctcccctgat aacggggagg tactcataat cgactgttta cctgcaagtc
     7561 atgtacaatc cctacgccgg cattatccgg atcaggttcc aaacagattt actgttttag
     7621 ggttttgaac atttgagtag ttcgaatctc agcctaattc attaagctcc ccataaaatg
     7681 atagtagcac aggcattaaa aattgtcaaa ggatttttgc cgaatcccgg ctcagactat
     7741 ccaaatccgg gacaaaaatt taaaaagaat tccggggacg gactaaaact ccccggaatt
     7801 ttatgatttt tactgcttaa ttatttcgta tgtacctctt accatgaaaa tggcgtagtc
     7861 atttgtatag gctccacgac cgtcacccct gggtacaatc atcagatgat tggaaacaaa
     7921 ctccattccc gaaacgaaat ccttgccgac cgtaacatct gaaattcccc ctaattcgct
     7981 tcctattaca aaagctttgc cgcctcttaa tataagctct gtgccggctc ctgccaccag
     8041 cctttcacct gcacgtattt caactatctg caaggcagct gaagagccgc ctgtaccgcc
     8101 gactgtcaaa tcatcaactt ttttcttcaa attttccagt tcgtccaatt tgtaatcgct
     8161 taccagtgcc ttgattcggt cattcaaata gcttaaaaca acgatggggt cattggaatc
     8221 tcccggagca gcgtaaatta ccgcagtaaa tgccaatgct aaaattaatg taaatatgat
     8281 tatttttttc atacagcaac tccttaaaag tatattgata ttttacatta aataagtata
     8341 aatttcaaca taaaacgaca gatatctttt gaaaaaaatt cctctaaaaa attttctcca
     8401 aagctatccg gcaaagttga caaagcacct aactttgcat tgaattaaac atagcgaaat
     8461 gatataattg cagggaagac ggcaaatgta ccaggagaca ataccaggga gaagtaaaca
     8521 tgtataaatt atgtatgaca gtaaacaaat tatttaagct gccggtgcac ccctttaatt
     8581 tacataatga aggcaaaaaa acatacgctc agtggcagta tgaaaaggga atggatacca
     8641 ttaagtttta ccttaagcac agcactgttg atgaaatgtt taaggataag attgttttgg
     8701 atattggctg cggtgcagga ggaaaaactg ttttttatgc ctcaaaagga gttaaaaaga
     8761 taaccggtat agaaattttg ggaaaataca aagaagaagc agaaaatctg gcaaaagagt
     8821 ttaatctcca ggataaattt gaattcgtcc aaggtgatgc agcaaacatg ccctttgaaa
     8881 atgaagcatt cgacacaatt ataatgaatg atgcaatgga gcatgttgac aagccggaaa
     8941 tggtgctggg agaatgctat cgtattttaa aaaaggacgg gaaactatat ttgaattttc
     9001 ctccctataa tcacccctac ggtgcacact tgagcgatgc cattggaata ccatgggtcc
     9061 atgtgttttt cagcgagaaa actctaataa aaacctataa ggacctggtt aaagacctgc
     9121 ctgacggaaa agagagaata gattttcgta taagcaaaaa agaagacggc acagagtatt
     9181 tttcatatat aaataaaatg tcaattaaaa gagcaaacac cattttacat aacagcagtt
     9241 ttgaattaga atattattcg gaggagcccc tgagaagctt ttttaaatat acggcccgca
     9301 tcccgttttt aaaagaattt ctggttaaaa tggtcgtttg tgttttgagg aagatgccct
     9361 aaccctgttt ttcctgtcaa atgggaagaa aactagcagc agtaattccc aacgattatg
     9421 atttacccaa gttcatcgac cggagggaga aagaaatagt tggtatatta agcgcaacga
     9481 attctgaatt tatgcggcgg taggagctga taaattcatg aaggtcgact gcgattccct
     9541 gtttatgcaa tgtaaggaac aaataaatta gtgaaggaga ctgcggtatc ccgtgaaatt
     9601 ttactataca tataggagaa aagatgaaac tgaaagttga taaaatagtt ttgattttga
     9661 tttgcctttc actgatagta ggtctgtata cactgtatca gagagtggaa attgaaaagc
     9721 agtataaaac tacggaaata gttcttgatt ataatgaaat gaagaaattt gccgacagtt
     9781 caaataatga cttaagctac tggatgagaa agtttaggga atttggagca aaatctgttg
     9841 caatacagga agaaaccatt aaattattga ttgaagccgg ttacagcctg agagctcaaa
     9901 tggtttcaga attaattaaa gaatacaaat ggcaggagga ttataaccgg gaaattgtaa
     9961 gagccatttc agaaaatgaa ataagtccct ttgatgttat tgttacaacg gaagatgaag
    10021 aagtttacag ctatatccta tcaggcctgg aagaaagata tgctgatgac ttttaccaaa
    10081 cacatatata cgacaatgtt tattacatag tattaaaggg tatagttgat gatgtatact
    10141 acaccgaatt ggaaaggact gtaaatttag caggcaaagg agtctatgag gttcaaaagg
    10201 cagttgattc aagactgatg aacataggta tcggatatga ttctgaaaaa ataaccatgg
    10261 caaaggatgc aggcctggat gtaatactaa gaccaattaa cttccctgca tcaaatgaaa
    10321 agctggctga tgcttataag gcagccaacg aaaaatatgg cctggagccg agaatttacc
    10381 tggttcacgg caaggaagtc ctgggttacc cgaataatga agacaaactg ttaaattaca
    10441 taaaagaaga gaatattgca gttgcattga ttgaaagcgg cacacaaagg cagcacctgg
    10501 agcaggaagg tctggatgag ctggttgaag attccggcta tcaagctctc cgtgcattta
    10561 ccatgtggga ttttataaga gaaagaaata gatactacaa ttatgaaggt gcagaagaaa
    10621 ttgaaaatac aatgtttagg gctgtgaccg aaaggaatat aagagttatt tatttcaagc
    10681 ccttctttga ggaaaaggac agcacgaaat ttcttacaga tgttgacgaa tatgaaagaa
    10741 catttaaaag tttggaaaac agactctctg cccacaatat aagttttgga aaagccaagg
    10801 ctatggaata cttcaccatc ggctccaaga gaatggctgt gcttgccttt ggaattaccc
    10861 tggctgcagt gatgctgttt ataaaaatgt ttaatattaa gcataaattt gctgattact
    10921 tatacttatt tgctctgcct gcagcactga tacctttggt tatgcggaag cttgcagaga
    10981 aaggatttgc ctttggctct gctgtagtat tttcaggact tgcaatttac ttttttatga
    11041 ttcagataaa aaggatttac gaatctgaag aaaagtattc aaatttgcag attattgttt
    11101 attcagccct gatacttgca ggcagcgttg caattgcgct ggcaggtgcg gtatttgttg
    11161 tttcaatgct tgcagatgta aaatatatgc ttgaaatgga tattttcagg ggagtaaaat
    11221 ttgcccagat actgcccttc ggtatttt
//
LOCUS       DAOPUT010000050        10988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_6024_length_10988_cov_18.702095, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000050 DAOPUT010000000
VERSION     DAOPUT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10988
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6024_length_10988_cov_18.702095"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKK75_05580"
     CDS             <1..242
                     /locus_tag="PKK75_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83104.1"
                     /translation="TFKPEQDITRAEAMTLINNVLERGVPEENIHPDAIFWPDNPNAE
                     WYYEAVMEATNSHDYVYEDDGDELWTGMKPNKVWP"
     gene            449..1915
                     /locus_tag="PKK75_05585"
     CDS             449..1915
                     /locus_tag="PKK75_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ83105.1"
                     /translation="MESTKKWKCLVCGEVVEGETPPEQCPVCGVGPEMFEELKEEKKE
                     AGEQKVYKWKCTVCGEIVEGLEPPEICPVCGVGPEYFVKMEEAEEEKVSDEKENIVII
                     GASGAGMGAAVEIRKRNKASDITILSKEGVKGYYRPQLTKMLSRNDVTIEQLAIKTDD
                     WFKENKVKLLLNKVVERIDTVNSKVILKDNEEIPYTKLIIASGAEVFVPPLEGRDKKG
                     VFTLRYVKDGDEIKDYAKGKNTAAIIGGGILGLEAASELNKMGLEVTVLERGDRILPR
                     QLDHDASKVLEQIVDNVGVNFLKFVNTKEIIGDEAVKGILLDNGETVQADVVIISTGV
                     KANTEIAKDTGIEIKRAIVVNERMETAVPGIYACGDCAEYEGINYALWSEAIEQGKTA
                     GINAAKGDYTYSTIIPSTTLNAFGTSVFSIGDIGSDPEAEYKTYEGNDSKSYIKLYFK
                     NNILAGGILIGDTSKTVVLINGFEKSKTMDEMIEKFKA"
     gene            2133..3014
                     /locus_tag="PKK75_05590"
     CDS             2133..3014
                     /locus_tag="PKK75_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HNZ83106.1"
                     /translation="MVYYVEYVFVENFIIDFILLFITGNLIKRAIIFKRLVIASLVGS
                     LYVIITYSTGREFMTYFIVKFSISVLMIMIAFDSKGILTNIRVIICFYITSLIMVGII
                     SALYLLTYDKLTVNAIIMSIFTGYAALHFFFREIKDRILRHNYKRTVKLRIGDKTKTF
                     RGWIDTGNELTDPVTGKPVMVVNMDCIRDIIGDGICTKIKDFYRDKSYENLIEENNCI
                     NLRIIKYNTISKKGENMVCIVPDEIEIINNSKSIFIDALIGIHPERISKDDYDALLFK
                     KLLDWEMETENGYVDVR"
     gene            2992..3714
                     /gene="sigE"
                     /locus_tag="PKK75_05595"
     CDS             2992..3714
                     /gene="sigE"
                     /locus_tag="PKK75_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HNZ83107.1"
                     /translation="MDMLMYVKAFFKRVFNFIIDKLDAVKDIFYIGGSDTLPPPLTQE
                     EEREIINRLKDSNEAKTILIEHNLRLVVYLAKKFESTGINVEDLISIGTIGLIKAVNT
                     FKSEKNIKLATYASKCIENEILMHLRRVGKTKTEISFDEPLNIDWDGNELLLSDILGT
                     EEDVVFRDMENEVDLCLLNDSIQKLNKREYMIMKLRFGLNNTKSYTQKEVADMLGISQ
                     SYISRLEKKILNRLKKDINKAV"
     gene            3778..4548
                     /gene="sigG"
                     /locus_tag="PKK75_05600"
     CDS             3778..4548
                     /gene="sigG"
                     /locus_tag="PKK75_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HNZ83108.1"
                     /translation="MNKVVICGVNTSQLPTIKTSEMRGMIKKIQEGDTELREKFIKGN
                     LRLVLSVIQRFNSRNEPVDDLFQIGCVGLIKAIDNFDLSLDVKFSTYAVPMIIGEIRR
                     YLRDNNSIRVSRSMKDTAYKALQVRERLTAENSQEPTIAEIAKEVGVDKEEIIFALES
                     IQEPVSLFEPIYNDGGDALYVVDQVKDEKNIDEKWIEKITLEDSINNLSEREKQIVDM
                     RFYKGKTQMEVADEIGISQAQVSRLEKSALNQLKKGFK"
     gene            4720..5004
                     /locus_tag="PKK75_05605"
     CDS             4720..5004
                     /locus_tag="PKK75_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HNZ83109.1"
                     /translation="MTNYWDLAEKDVINIKNGEVMGRFDDVEIDTRKGRITAFYIEEA
                     SKFIGMLGKTKPKRIRWEEILKIGMDVIIVNVDDDKNNKQIKDMLEYREA"
     gene            complement(5071..6231)
                     /locus_tag="PKK75_05610"
     CDS             complement(5071..6231)
                     /locus_tag="PKK75_05610"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNZ83110.1"
                     /translation="MKYISILGSTGSIGTQTLDVVREHNDKLKVSAISGNNNIELLKE
                     QILKFAPDLCCVMDDDNALKLKKILPSHIKTEILSGMEGLISIAEYEKSQIIVTAVSG
                     MIGLRPTIAAIKKGKTIALANKETLVTGGNYIMDLSKQFKAPILPVDSEHSAIFQCLM
                     ANENKAVNKILLTASGGPFRGKNDEFLKTVTVEDALNHPSWSMGKKITVDSATLMNKG
                     LEVIEAKFLFGIEPEQIEVIVHPQSIMHSGVEFIDHSTIAQLGLPDMRIPIQFALFYP
                     NRMKNGYKSLSLSDIAALTFEKTDLKTFKCLSLAYEALEAGGTMPAVLNASNEACVKL
                     FLDKKISFLDIGNINEKVMLAHSPEKIDSLETILQAEEFAKQMVIKLISSGR"
     gene            6559..8076
                     /gene="thrC"
                     /locus_tag="PKK75_05615"
     CDS             6559..8076
                     /gene="thrC"
                     /locus_tag="PKK75_05615"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003147260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNZ83111.1"
                     /translation="MGRSYESTRNPELKAAASEAVLKGIADDGGLFVMRDLGEKKVVI
                     KDLIGKNYMEIAEKVLGILLDDYPEHIIKECISNAYRDKFSTEEITPLVKAGENYVLE
                     LYHGLTSAFKDVALSILPHLMTTSYEMNRMEGEVIILTATSGDTGKAALEGFKDVKGT
                     KIIVFYPEGGVSQVQEMQMVTQEGNNTFVCAVEGNFDDAQTGVKNIFANEKFNRELAE
                     KNKFLSSANSINIGRLAPQVVYYFVSYVRLVEKGLLEIGDKVNFSVPTGNFGNILAGY
                     YAKMLGLPVNKLICASNENNVLYDFIKTGVYNKNRKFFKTISPSMDILVSSNLERLLY
                     YLSGKNNKLVKDYMEKLNTSGIYEITAEMAEKLGDEFYGGCVYKEEVKGTINHTYRKF
                     GYLLDTHTAVAYKALEDYKTETGDDTASIVLSTASPFKFSKSVYEALFGKADDEYEEF
                     ILMEELSERTKVSIPENLKHLEYKKIRHFEVCSVDEMEGYVADASYESDNEVENG"
     gene            8069..8950
                     /gene="thrB"
                     /locus_tag="PKK75_05620"
     CDS             8069..8950
                     /gene="thrB"
                     /locus_tag="PKK75_05620"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNZ83112.1"
                     /translation="MVKVTAPATTANIGPGFDVFGLAFKLYNEYTFEKADEELIIEGC
                     PEAYNNKENLIYKSFAVTAEKLGQKAEGLKISVKTNIPVSRGLGSSSACITGGVYGAN
                     AFYGGGLTREEMYKIAVMIEGHPDNISPCVYGGLTASMIDEEIPYCVEYDISSELMFC
                     ALIPDFEVSTSEARKILPKMVDHKDAVFNVSRAVVLLKALETGNLEMINISLNDRLHQ
                     KYRGGLIHEYDELKAICAQAGSPALFISGSGPTLMNISKDEMFPEKIKDRVAGLKHKW
                     EIKVLGIDKKGAFTEVF"
     gene            9041..9475
                     /locus_tag="PKK75_05625"
     CDS             9041..9475
                     /locus_tag="PKK75_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNZ83113.1"
                     /translation="MKKYLIVNKKILPDVYDRVIEARNLINGGSVKGISEAVKVVGIS
                     RSTYYKYKDYVFSPDENSIGRKAVFSMMLKHEKGMLSNVLNYLSLQNANILTINQSIP
                     INGKASVTVSLDISDLSKSIDEVMAEMKKIKGATQVRLLSVE"
     gene            9488..10696
                     /locus_tag="PKK75_05630"
     CDS             9488..10696
                     /locus_tag="PKK75_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ83114.1"
                     /translation="MYGLVLEGGGAKGAYHVGAYKAIRELNIEIGGIAGTSIGAINGA
                     MMVQGDYELLEKVWCSVNSYELFDIDEKAIEDLRRFNLHDVNFSYLLHQSKEILGNRG
                     LDTSRIRELFDSYIDEDKIRSSNIDFGIVTVNLTDRKPMELMKEDIPEGKLVDFLIAS
                     ANLPAFKIEEMDGKKYLDGGFYNNLPIDILANRGYKDIIAVRTLAIGVVRKARVKGLN
                     VTFIQPFESLGTMFGALDFNRERAKEFINLGYYDAMKVFKKLRGYKYYIIPFEGSFIN
                     ILVDFFNEYKEKLCFVGHFLGYGEVSEDRMFFEKILPRMEQILDMKGKYDYQDICIRF
                     FERIAEKYQVERFKIYKAEEFIELTIEKFKEAPTAFIKNVPNFIKQNRILSLAVKDDL
                     IVEIFAELFL"
BASE COUNT         3600 a         1689 c         2548 g         3151 t
ORIGIN      
        1 tgacctttaa accggagcag gatatcacaa gggctgaagc aatgacattg attaataatg
       61 ttcttgagag aggcgtacct gaagaaaata ttcatcctga tgctatattc tggcctgaca
      121 atccgaatgc tgaatggtac tacgaggctg taatggaagc taccaacagt catgattatg
      181 tatatgagga tgacggagat gaactctgga ctggaatgaa accgaacaaa gtatggccgt
      241 aacaaggtat ggggatatac ctctaagtat aggataagtt cctgtggaca tgaggaatgt
      301 cccccataat tgataagtat ggcggagcga aagctccgca tatttttttc tgccatttac
      361 aaaattggga atataggtta aaataatgac attaagttta taattttaaa gaccggataa
      421 tataacagta ctgtgatgag gagggtttat ggaatcaacg aagaaatgga aatgtttagt
      481 ctgcggggag gttgtggaag gtgaaactcc gccggaacaa tgccctgtat gcggtgtggg
      541 tcctgaaatg ttcgaagaat taaaggaaga aaaaaaagaa gccggtgagc agaaagtata
      601 taaatggaaa tgcacggttt gcggagaaat cgtggaagga ttagaacctc ccgagatatg
      661 tcctgtatgc ggagtaggtc ccgagtattt tgtcaaaatg gaagaagcag aagaagaaaa
      721 agtttccgat gaaaaggaaa atatagtgat aatcggcgca agcggtgcag gtatgggagc
      781 tgccgttgaa ataagaaaga gaaacaaggc gtctgatatc accatattgt caaaggaagg
      841 cgtgaagggt tattatagac ctcagctgac gaagatgctg agccgcaatg atgttactat
      901 tgaacagctt gccatcaaga cagatgactg gtttaaggaa aacaaggtaa agctgcttct
      961 caacaaggtt gttgagagaa tcgataccgt aaattcgaag gttattctca aggataatga
     1021 agaaattcct tacaccaagc tgattattgc ttcgggagcc gaagtttttg ttcctccgct
     1081 tgagggaagg gataagaaag gtgtatttac attaagatat gtgaaggacg gcgatgaaat
     1141 aaaggactat gccaaaggaa aaaatacagc tgccataatc ggcggcggga ttcttggttt
     1201 ggaggctgcc agtgagctta ataaaatggg ccttgaagta actgtccttg aaagaggcga
     1261 caggatactt ccgagacagc ttgaccatga tgcatccaag gtactggagc aaattgttga
     1321 caatgtcggc gttaattttc ttaagtttgt gaatacgaag gaaattatcg gtgacgaagc
     1381 cgtaaagggc attttgcttg acaacgggga aactgtccag gctgatgtgg taataatttc
     1441 aacaggagtt aaagccaata ctgagatagc taaggatacg ggaattgaaa taaaaagagc
     1501 aattgtggtt aatgaaagga tggaaactgc tgtgccgggt atttatgcct gcggtgactg
     1561 tgcagaatac gaaggaatta attatgcgct gtggagcgaa gctattgagc agggcaagac
     1621 tgcagggata aacgctgcta agggggatta tacatattct acgattattc cttcaacaac
     1681 tttaaatgct tttggtacca gtgtgttttc aatcggtgat atcggttctg accctgaggc
     1741 ggaatataag acttatgaag gaaatgacag taaaagctac ataaagctgt attttaagaa
     1801 taatattttg gcagggggaa ttttgatagg agacacatca aagactgtag tcttaattaa
     1861 cggttttgaa aaaagtaaaa caatggatga aatgatagaa aaattcaaag cataatttaa
     1921 gtagtttact atacaaaata cgcggtgata ttcataagcc gcgtattttt tttatatcag
     1981 gctacatgtt atgacaaaat atttacaaag agtcatttat aatggggaat aggtttaaaa
     2041 tcagaatggg gacataaaaa aaggtatgga aacacacaca gggcagcctt tgtatgcagt
     2101 ggaatgtttc catattacgg gagttgtaag tattggttta ttatgttgaa tatgtctttg
     2161 ttgaaaattt catcatagat tttattttgc tgtttattac aggcaatctc ataaaaaggg
     2221 caattatatt taaaaggctt gtaattgcat cacttgtcgg ctccctgtac gtaattataa
     2281 cctatagcac cggaagggaa ttcatgacct atttcattgt gaaattcagc atttcagtgt
     2341 tgatgataat gatagcattt gattccaagg ggatattgac aaatatcagg gtaataatct
     2401 gcttttatat tacttccctg ataatggtgg gaattatttc tgccttatat ttattaactt
     2461 atgataaatt gaccgttaat gcaattatca tgtccatatt tacagggtat gcagcattgc
     2521 atttcttttt tagggaaatt aaagacagga ttttaaggca taattacaaa agaacggtaa
     2581 aattaaggat cggagataaa acaaagacat tcaggggctg gattgatacg ggcaatgaac
     2641 ttacagaccc ggttaccggg aagcctgtaa tggttgtgaa catggattgt ataagggata
     2701 ttatcgggga tgggatttgc acgaaaatca aggattttta ccgggataaa agctatgaaa
     2761 atttaattga agagaataac tgcataaatt taagaattat taaatacaac actatcagca
     2821 aaaaaggcga aaacatggtc tgcatagtgc cggatgaaat tgaaataata aataacagca
     2881 aaagtatttt tatagatgca cttattggta tacatcctga aagaatcagc aaggatgatt
     2941 acgatgcact tttatttaag aaattacttg attgggagat ggagacggag aatggatatg
     3001 ttgatgtacg ttaaagcatt ctttaaaaga gtatttaact ttataataga taagctggat
     3061 gcagtaaagg atattttcta tataggagga agcgacacct tgcctccacc cttgacccag
     3121 gaggaagaaa gggaaataat taacaggctt aaggattcca atgaggcaaa aacaatttta
     3181 atcgagcata atttaaggct tgttgtgtac cttgcaaaga agtttgagtc tacgggaata
     3241 aatgtggagg atttgatttc catcggaacc atcggattaa taaaagcagt taataccttt
     3301 aagtcggaga aaaatatcaa gcttgcaacc tatgcttcca agtgcattga aaatgaaata
     3361 ttgatgcatt taaggcgggt agggaaaacc aagacggaaa tttcatttga tgaacctttg
     3421 aatattgact gggacggaaa tgagcttttg ctgtctgata ttttaggcac tgaagaggat
     3481 gttgtattcc gtgacatgga aaatgaagtg gatttatgcc tgctgaatga ttcaattcaa
     3541 aaattaaata aaagagaata tatgattatg aaattaaggt tcggccttaa caacaccaag
     3601 agctacacgc agaaggaagt ggctgatatg cttggaatat cacagtctta catttcacgg
     3661 ctggaaaaaa agatactaaa cagactaaaa aaggatatta acaaagctgt ataaaaagat
     3721 acagccgagg aaataatttt aacgggggat aatttcttta aaggggaatc gacgattatg
     3781 aacaaagtag ttatatgcgg cgtcaatact tctcagctgc ccaccataaa gacatcggaa
     3841 atgagaggta tgattaaaaa aatccaagaa ggagacactg agcttagaga gaagttcatc
     3901 aaagggaatt taagacttgt tctaagcgta attcaaagat ttaattcaag aaatgagcct
     3961 gttgacgatt tgtttcaaat tggatgcgtc ggattgataa aggctattga caattttgat
     4021 ttgtccctgg atgtgaagtt ttccacttat gctgtcccca tgataatcgg agaaataaga
     4081 agatatttaa gagacaataa ctccataagg gtgtcaagat cgatgaagga taccgcctat
     4141 aaggcattgc aggtgaggga aaggctgacg gctgaaaact cacaggagcc cacaattgct
     4201 gaaattgcaa aagaagtggg tgttgacaag gaagaaatca tatttgccct ggaatcgatt
     4261 caggagcccg tatccttgtt tgaacctatt tataatgacg ggggagatgc tctttacgtg
     4321 gtggaccagg taaaggatga aaagaatata gatgagaagt ggattgaaaa aataaccctg
     4381 gaggacagca taaacaacct gtctgaaaga gaaaagcaaa ttgtggatat gcgcttttac
     4441 aaggggaaaa ctcagatgga ggtggcggat gaaataggga tttctcaagc ccaggtatcc
     4501 cgcctggaaa aatccgcact gaaccagctt aaaaaaggct ttaaatgaca agagaacggg
     4561 gggacaggga gcgcacagcg ttcctttttt atttaaaaat ttaccatatt tatatatagg
     4621 gatccaagtt tcactattat ttattgtaca atgtttgtca tatttgataa ttcttgtaca
     4681 tattttataa tatacaagaa ggattaaggt ggtgattttg tgactaatta ctgggattta
     4741 gctgaaaagg atgttattaa tataaaaaac ggcgaagtta tgggcaggtt cgacgatgtg
     4801 gagatagaca caagaaaggg aagaattact gccttttata tagaggaagc aagcaagttt
     4861 attggtatgc ttgggaaaac aaaaccaaag aggatcagat gggaagaaat attaaaaata
     4921 ggcatggatg taataattgt caatgtggat gatgacaaga ataacaaaca aattaaggat
     4981 atgttagaat atcgagaagc ttaagtattt tagggatgtg tcttggggtg atttttttaa
     5041 ctctcaaagg tccgttttca gaatcactta tcaccgccca ctgcttatca attttatcac
     5101 catttgtttt gcaaactctt ctgcctggag gattgtttcc aatgaatcaa ttttttcagg
     5161 ggagtgggca agcattactt tttcgtttat gtttcctatg tcgaggaaac ttattttttt
     5221 gtccaaaaac agctttacgc aggcttcgtt tgaagcgttt aatactgcag gcatggtgcc
     5281 gccggcctct aatgcttcat atgccagcga taggcatttg aatgttttta aatcggtttt
     5341 ttcgaatgtt aaggcggcta tatctgataa agacaggctt ttatagccgt ttttcatgcg
     5401 atttggataa aagagcgcaa attgtatggg gatgcgcata tcaggcaggc caagctgtgc
     5461 aatggttgaa tggtcaataa attcaactcc cgagtgcatg atgctttgag ggtggacaat
     5521 aacttcaatc tgctccggct ctatgccgaa taaaaactta gcttcaatta cctccaaacc
     5581 tttattcatt aatgtggcag aatcaaccgt aatttttttg cccatggacc agctgggatg
     5641 gttgagggca tcttccacag taacggtttt taaaaattca tcatttttgc ctctgaaggg
     5701 tcctcctgaa gctgtcagca atattttatt gaccgctttg ttttcatttg ccatgaggca
     5761 ttggaaaata gcgctgtgct cgctgtcaac gggaagtatg ggggctttga attgttttga
     5821 taaatccatt atgtaatttc cgcctgttac cagagtttcc ttattggcaa gagcaattgt
     5881 ttttcctttt tttattgcag cgatagttgg tcttagaccg atcatgccgg atactgcagt
     5941 aacgattatc tggctttttt catattctgc aatggaaatc aaaccttcca tgcctgaaag
     6001 tatttctgtt tttatatgag aaggcagaat tttttttaat ttcagtgcat tatcatcatc
     6061 catcacacag cacaggtcag gggcaaattt taatatttgc tcttttaaaa gttcaatatt
     6121 gttgtttcct gaaattgccg ataccttcaa tttatcatta tgttctcgta caacatccag
     6181 tgtctgtgtt cctattgaac ctgtggaacc tagaatactt atatatttca tggctgctcc
     6241 ttgttattaa tagtagagag gtcttttctg atacgttcat tttatcatat gtatttatta
     6301 tttgaaagaa attcttgcaa tacccccgcc ggaaaaatag aacgcccgtg gtaaacacct
     6361 ctgtcaacat cctcgtcatc accaacggcg cacaccccac gtcatgtaac tgtacccccg
     6421 tcccccagtc atatgtggga cagtttagga ccgtccccag tgtcccactc acgcccctgt
     6481 gtcatcttaa aaaaacactt gtaattatat cgagaacagt atatcatatg tttattatat
     6541 atgaagacgg aggaactaat ggggaggagt tatgaaagca ccagaaatcc tgaattaaag
     6601 gcggctgctt cggaggctgt attgaaaggt attgcagatg acggcggtct gtttgtaatg
     6661 agggatttag gcgaaaaaaa agtagtcata aaggatttaa tcggtaaaaa ttacatggaa
     6721 attgctgaaa aagtgctggg tattttgctt gatgactatc cggagcatat aattaaagaa
     6781 tgtatcagta atgcgtacag ggataaattc agcactgaag aaataactcc tttggtaaag
     6841 gcaggagaaa attatgtatt ggagctttac cacgggctta cttcggcatt taaggatgtg
     6901 gctctttcaa tattgcctca tctgatgaca acttcttatg aaatgaaccg catggagggc
     6961 gaagtgataa tcctgactgc aacctcaggg gacacaggca aggctgcact ggaaggattt
     7021 aaggatgtta aaggaacgaa aattattgtg ttttatcccg agggcggtgt aagccaggtc
     7081 caggaaatgc aaatggttac acaggaaggg aataatacat ttgtctgtgc tgttgaaggg
     7141 aactttgacg atgctcagac cggtgtcaaa aatatttttg caaacgagaa attcaacagg
     7201 gaattggctg aaaaaaacaa gtttttgtct tctgcaaatt ccataaatat agggagactg
     7261 gctccccagg tggtttatta tttcgtttca tatgtcaggt tagtggaaaa aggccttttg
     7321 gaaattgggg acaaggttaa tttttcggtg cctaccggga attttggaaa tattttggcg
     7381 ggatattatg caaagatgct ggggcttcct gttaataagc ttatatgtgc ctcaaatgag
     7441 aacaatgttc tctatgattt cataaagaca ggagtttaca ataaaaacag aaaatttttc
     7501 aagactattt ctccatccat ggatattttg gtatccagca atctggagag gctcctgtat
     7561 tatttaagcg gcaaaaacaa caaacttgta aaggattata tggaaaaact aaatacttcc
     7621 ggcatatatg aaataactgc agagatggct gaaaagctgg gggatgagtt ttacggagga
     7681 tgtgtttata aggaagaagt taaaggaacc attaatcata catatagaaa attcggatat
     7741 ctccttgata ctcatacagc ggttgcatat aaggcactcg aggattacaa gacagaaaca
     7801 ggggatgata cagctagtat cgttctttca actgcaagtc cctttaagtt ttccaaaagc
     7861 gtttacgaag ccctgttcgg gaaagcagat gacgagtatg aggaatttat attgatggag
     7921 gaattgtccg agagaacaaa ggtaagcata cctgaaaact taaagcatct tgagtataaa
     7981 aaaataaggc attttgaagt atgcagcgtt gatgaaatgg aaggctatgt ggctgatgct
     8041 tcttatgaat cagataatga ggtggaaaat ggttaaggtt actgctccgg cgacaactgc
     8101 caatataggg cccggttttg acgtgtttgg actggccttt aaattatata atgaatatac
     8161 ctttgagaaa gctgacgagg agctgataat tgaaggatgc ccggaggcat ataataataa
     8221 ggaaaatttg atttacaaat cctttgctgt tacagcagaa aaattagggc aaaaagctga
     8281 agggctgaag atttcggtga aaaccaacat acccgtttca aggggactgg gcagcagctc
     8341 tgcatgcatt acgggaggcg tctatggtgc aaatgctttt tacggcggag gacttacaag
     8401 ggaagaaatg tataaaatag ccgtaatgat tgaaggacat cctgataata tttctccctg
     8461 cgtctatggg gggctgacag cgtcaatgat tgatgaggaa ataccttatt gtgtggaata
     8521 tgatatcagc agcgagctta tgttttgcgc cctgattcct gattttgaag tttcaaccag
     8581 tgaagcaaga aagattcttc ccaaaatggt agaccacaag gatgccgtat ttaatgtatc
     8641 aagggctgtt gttttattaa aggcattgga gacaggcaat ttggagatga ttaatatttc
     8701 acttaatgat agactgcacc agaaatacag gggcggccta attcatgaat atgacgagct
     8761 aaaagcaatc tgtgcccagg caggttcgcc ggcactgttt atcagcggtt cagggcctac
     8821 attgatgaac atatcaaagg atgaaatgtt tcctgaaaag attaaagaca gggtagccgg
     8881 tttaaaacac aaatgggaaa taaaagtgct tggaattgat aaaaagggag cttttacgga
     8941 ggttttttag aattcaggca tctatgatgt gtttttaatt acagattcag gagttaaata
     9001 ataaaataca ataattaata taataatcga ggtggataca atgaaaaagt atttgattgt
     9061 gaacaagaaa attcttccgg atgtatatga cagggttatt gaggcaagaa atttaatcaa
     9121 cggcggaagc gtgaagggca taagtgaagc agtcaaggtt gtaggtataa gcaggagcac
     9181 atattacaag tacaaggatt atgttttttc tccagacgaa aactcaatag gaaggaaagc
     9241 cgtgttttca atgatgctca agcatgagaa ggggatgctc tcaaatgttt tgaattattt
     9301 gtcattgcag aatgcaaata ttcttaccat aaatcaaagc attccaatta acggaaaggc
     9361 atcagtaact gtatcccttg atatttccga ccttagcaag tccattgatg aggtgatggc
     9421 ggaaatgaaa aaaataaagg gtgcaaccca ggtgagatta ttatcagttg aataaatggg
     9481 tgatattatg tatggattgg ttttagaagg cggcggtgcc aagggtgcat accatgtggg
     9541 agcatacaag gccataaggg aattaaatat agaaatcggg ggaattgccg gtacatcaat
     9601 aggagcgata aacggggcaa tgatggttca aggggactat gagcttttgg aaaaggtttg
     9661 gtgctctgtt aattcatatg aattatttga tattgacgag aaggcaatag aagatttaag
     9721 gcgattcaat ctccatgatg ttaatttttc gtatcttttg catcagtcta aagaaatcct
     9781 gggcaacagg ggtttggata cttcaaggat tagagaattg tttgactcat atattgatga
     9841 ggataaaatc cgaagttcta acattgattt tggaatagta acggttaacc ttaccgacag
     9901 gaagcccatg gaactgatga aagaggatat accggaggga aaactggttg attttttaat
     9961 tgccagcgca aatcttcccg cctttaaaat tgaagaaatg gacggcaaga aatatttgga
    10021 cggtggattt tataataatc tccccataga tattttagca aacagaggtt ataaggatat
    10081 tatcgccgta aggaccttgg ccattggtgt tgtgaggaag gccagggtga aaggtttaaa
    10141 tgtcaccttc atccagccgt ttgaaagtct ggggacaatg ttcggtgctt tggatttcaa
    10201 cagggaaaga gcaaaggaat tcataaatct gggttattat gacgccatga aggttttcaa
    10261 aaagctcagg ggctacaagt attatataat tccttttgaa ggaagtttta taaacatact
    10321 ggtggatttc ttcaatgaat acaaagaaaa actgtgtttt gtaggacatt tccttggcta
    10381 tggcgaagtg agcgaagaca gaatgttttt tgaaaagatt ctgcccagaa tggaacaaat
    10441 actggatatg aagggcaaat atgactatca ggatatttgc ataaggtttt ttgaaagaat
    10501 tgcagaaaaa taccaggttg aaaggtttaa aatatataaa gctgaggaat ttattgaatt
    10561 gacaattgaa aaattcaaag aagctccgac ggctttcatt aaaaacgtac cgaatttcat
    10621 taaacaaaac agaattttat cacttgcggt taaagatgat ttgattgtgg agatttttgc
    10681 agagctattc ctttagatca catggggacg cacctttaac tcaagataag tcttcaaggg
    10741 caggcattaa aggaatgtcc ccggtttgga gcggagacgt gggtgctttc atatgacagc
    10801 agccaggctt tctaaataca aaacgggaca gggtgtcccg tttctgcttc ttatttagtt
    10861 ttcaattcat aaaataatca attatcccgg cacctatttt ttccaccata acattttgga
    10921 agctcgtgtt gtaaatattc atttcttctg tggggtttga cataaaccct atttcaatca
    10981 gtgctgca
//
LOCUS       DAOPUT010000051        10947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_6055_length_10947_cov_19.278828, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000051 DAOPUT010000000
VERSION     DAOPUT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10947
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6055_length_10947_cov_19.278828"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1293
                     /locus_tag="PKK75_05635"
     CDS             67..1293
                     /locus_tag="PKK75_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNZ83115.1"
                     /translation="MKIIQVAKDIHMLTMNVDDILFEGIWELANGVTLNSYIVKGEKT
                     AIIDGVIGWDGVPETLYDNLKEIGIAPDDIDYLIVSHMEPDHSGWIESFKKLNDSLTI
                     ICTDKAAKLVSSFYGNNVKISVVKEGDTLDLGKGKILSFHPVPNVHWPDTMYTYELST
                     KTLFSCDMYGAFGKMGEHHFDDEMTNDEIDFFEMEGIRYFSNVMATFTTAVKKAIQKT
                     EEMDINLIAPGHGPLYRKAPEKIIDDYKRFCQYAAGSGKNEISILWGSMYGMTEKAVN
                     FAESVLKREGIKVNKLKMPLESESEMVATVFKSAGIIIAAPTYEYKMFPPVAAAIDEL
                     GRKKISGKAAFRLGSYGWSGGAEKELKEIIERNNMKWDFVETVEFEGSPKAEDLNKIE
                     SRVLELIAKMKDKIVE"
     gene            1592..2908
                     /locus_tag="PKK75_05640"
     CDS             1592..2908
                     /locus_tag="PKK75_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ83116.1"
                     /translation="MYTKYFKRIIAFVIVLSMVFTSVAMAMPYGQAKKFIRNGYSWEK
                     VQKYVQGKGIMKGFPDGKFYENLYVKRADAIVMIDRAFKLNALIDSLNIDYEELFDDV
                     EVGEYYFHSIYIARALGITKGIGNNKFNPKHSVTIEEVILLIERAMGKNKYFEFDEDI
                     DLREDYKELVGKGPNGKTKDLKDFATRGEIAAMLYYVLTGSKYDGEPVDEEENEDKIT
                     RTMKENEVLKFDVKYDNKDSLIENIEDLIDDDLDYVKFTKPLNKDNSFLYYDYDEDDS
                     SHSFVIKGKEYYVKTIGKQMDLSEVTIVPKTTGVLKIEFTAYDKDDKPYDGIIEITVE
                     PMLGELLIDENDIDDFAEEVKGYLEEFDEYDFEEIIFEKLDEDDDYDLKIEGRIRSLF
                     GRNISFERLGKVKFIDGDDFDGTTSLKYTVTTDDGLKFTGLIEIDD"
     gene            3136..3429
                     /locus_tag="PKK75_05645"
     CDS             3136..3429
                     /locus_tag="PKK75_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HNZ83117.1"
                     /translation="MAIFNETVKRLIPTLEKFVPIVDRVHGEHHPEFHDVRKIFNTIN
                     EKAKEAGTEKPDLSREFAKLREITDNYTVPGDVCESYEAAYKMLSELDESYQA"
     gene            3564..4667
                     /locus_tag="PKK75_05650"
     CDS             3564..4667
                     /locus_tag="PKK75_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HNZ83118.1"
                     /translation="MNYLQLDTPALLINKGIMLDNIKHMQDYADEKNVALRPHTKTHK
                     MSKIALLQEEAGAKGITVAKVGEAEVMAANGLKDIFIANEIVGDIKLSRIKKLAETID
                     ISFGLDSIAQAAMVEKAFEGSKKANVLIEIEVGENRSGVIEEADYIELLNYLKKCSNI
                     NLKGVFSHDGHSYSAENIEACKKIHLEAQRRTLRFADIANEMGFKLETVSIGSTPSLL
                     HNFPILDGVTEIRPGTYIFMDASQAAAYGSFKRNAATILTTVISLPTPERVITDVGAK
                     GITMQARNKGFTATKGLGLIKGWPQVNIYAVYDEHAIIYNKELHDSIRVGDKLEIIPN
                     HICPVVNLYETAYIIENDRVVDEIPVDCRGKLK"
     gene            complement(4742..5662)
                     /locus_tag="PKK75_05655"
     CDS             complement(4742..5662)
                     /locus_tag="PKK75_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016510891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ83119.1"
                     /translation="MKTKLIADSTCDLSQEILNRYNICLAPLVVTIEGKEYKDRIDIS
                     NDEFYKNLMTFKTYPTTSMPSPESYIECIKGAIDQGYEKVLCICMSSGTSGSYQSALL
                     AKELFFEQNPDSNIEFQVIDSVSMSHGSGWLIMKCAMLIEEGYTFEELVEYCETIKYR
                     IKHFLSVDDLDSLIRSGRLSNASGVIGKLLNVKPIMSMKNTKGAVVAKKRGSKQVYNY
                     YVQEFINRVDYEQTNFVIVGYTSDINKAENLKALLIEAGFKGQVYIMQMGVAVGTHVG
                     LGGLSMFFVEKPHEDFINHMKEKIISHIHK"
     gene            5937..6293
                     /locus_tag="PKK75_05660"
     CDS             5937..6293
                     /locus_tag="PKK75_05660"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNZ83120.1"
                     /translation="MNNYIKGIKNKYVINKVQNTRIPDFKPSKISRSLIIFSGRVQKV
                     GFRLEIYELAKRLNLTGSVSNMKNGDVRLEIQGEKEKILFLTGHMRSLKRAKVRNVRF
                     QDEVLVNGESSFIYEP"
     gene            complement(6492..7889)
                     /locus_tag="PKK75_05665"
     CDS             complement(6492..7889)
                     /locus_tag="PKK75_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016114456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNZ83121.1"
                     /translation="MKKISVSLRPLVSNINLPTVIKTAVLPGDSYESIFIAGQVGEIY
                     NIRNGQIRLFLDIRQRIIRLGQNGGYDERGLLGLAFHPNFYLNGLFYLHYSAAGTQGP
                     GALSGSFQPNPCDPETLNLRWTNRDTQYDHIDTVEEWSLQSNGQAQKRRTLLNLRRPF
                     MNHNGVNSLNFSPETGRLVLTTGDGGSGYDPFNSAQNIMEVAGKIIEIDVNTDTAINN
                     LPVVTRFNELPEAVQRTLTVMAKGVRNIPGIAFQRYYNQYIKYAGNVGQDLIESIFTF
                     TSYKPIPVTEITQAANQSSDQEGFVNLGWRGWEGDLPTPIINSCLSNSSLVEKTTAYY
                     NEAISTAAKRLLPLTCYYHMDSRPDKFRGTALTGVQPYMGYDIPDLTGCIVFIDFARK
                     GESPVRGALAYTKVRTECRQYDYSLIDTNYNFGSQSAYYVSLGTNLTQSKLYLGVYGS
                     SNVTDLNQGTVFEIV"
     gene            8126..8656
                     /locus_tag="PKK75_05670"
     CDS             8126..8656
                     /locus_tag="PKK75_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNZ83122.1"
                     /translation="MTAEKLLIKNLKRGREEAYRQLIEEYGNRLLRTCCLILKDREEA
                     EDVVQETFIKVFNKIDTFKEKSGLYTWIYSIALNLSRDRMRLKQDMLELKDEWIGNDD
                     VESQVEINIDRELLRKEIFEMNSLYKEVLVLYYYEGLSIKEISYLLNEKEGTIKSKLF
                     RGRNILKEGLLKGGQK"
     gene            8653..8973
                     /locus_tag="PKK75_05675"
     CDS             8653..8973
                     /locus_tag="PKK75_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83123.1"
                     /translation="MNKKDKQDKLGMEIKDIMEEETFDLTLSQKAFDNIIKHRKKSRN
                     EKIREFLNRDIEIPLAPAIVGLAALFAITLIPGNVFKSQNEEIINMGNSQVIIREDFE
                     VSQK"
     gene            8970..>10947
                     /locus_tag="PKK75_05680"
     CDS             8970..>10947
                     /locus_tag="PKK75_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ83124.1"
                     /translation="MKIKIKIKTLILTVFVLALTFILAIPTATLMIAGFLDDRGSEKA
                     SLFYERYASYATTPNIKSNYLYADSLVKSFTKYTIFFTGWGGGANTSPEDMDKAKQIL
                     IETLAETPVKNSEKDYYIKSYKMLLDIAIATGDARMLREWIDFGQEVEDEKLIYTADI
                     YEGFLHHVNGESEAAKRVLEKYEDTESKDISYDVLKAEIALFDGKYEEVETIYENLYK
                     NNWRELGRSFGSGGYDRGHWFEERFDDLKGDSILRGTVTYEGKPMPFVEIYVQEATGG
                     LSLLSRGESYAGITDENGQFRTLGLKEGLYNVGIGVDGSILTDKELQSSTNKYVELDE
                     KGGEIHFIFRDTLKINSPKLGEKLSGDEFTVSWEEVENAAYYTVGAVVFYEPFGDKGI
                     STQTYLKDLNGEYKFTETNAVFNINLFRNNPNMNVSDSEKEYLGAQAVLGIFLPGVEY
                     PIVVNAFDENNNLITRSLPKISYYDQIPGITVEGSLTEGENLIYHKNYIEALEYYENI
                     LTENPDDTAALRYLTRIYGIGWKEGEQNLKRAFELGQRYSEISGDWNLLINTVAIMDM
                     SEIKENSNRVFEVLTEAMKDPNFDNYSFLSRYYIAVEDYEKARETLREWETVTDTLIF
                     LNIYFGDYMEASKNIKSESFYISRLSSNRVKGALE"
BASE COUNT         3862 a         1712 c         2212 g         3161 t
ORIGIN      
        1 attgaaaaca catgaaaaat atggtataat agaagaaaac caggttatta aaaaggagga
       61 atacaaatga aaataataca ggttgccaaa gacatccata tgcttaccat gaatgtcgat
      121 gatatacttt ttgaagggat atgggagctt gcaaatggcg ttacactaaa ttcctatata
      181 gtaaaaggag aaaaaactgc aataatcgat ggtgttatag gctgggacgg agtgcctgaa
      241 acactgtatg ataatctgaa agaaataggc atagcacctg atgatattga ctacttaata
      301 gtgagtcata tggaacccga ccattccgga tggattgaaa gctttaaaaa attaaatgat
      361 agcttaacaa ttatatgcac tgataaagca gctaaattag tatcatcttt ttacggaaac
      421 aacgtaaaaa tcagtgtggt aaaagaggga gacacacttg atttaggaaa gggcaaaatt
      481 ttaagctttc atcctgtacc caatgttcat tggcctgata ccatgtatac ttatgaatta
      541 agtacaaaaa cattattttc atgcgatatg tacggggctt tcggcaaaat gggagaacat
      601 cattttgatg atgagatgac taatgatgaa attgactttt tcgaaatgga aggcataaga
      661 tacttttcaa atgtaatggc tacatttacc actgctgtaa aaaaggcgat acaaaagaca
      721 gaagaaatgg atataaatct tatagctccg ggacacggac ccttatacag gaaagctccc
      781 gaaaaaataa ttgatgatta taagcgcttt tgtcaatatg cggcagggtc agggaaaaat
      841 gaaatcagca tattgtgggg ttcaatgtac ggcatgactg aaaaagcagt aaactttgca
      901 gaaagtgtgc tgaaaagaga gggcattaaa gttaacaagc tgaaaatgcc tttggagagt
      961 gaaagcgaaa tggttgcaac tgtatttaaa tccgcaggca taataattgc tgcaccaacc
     1021 tatgaataca agatgtttcc gccggttgca gctgcaatag acgagctggg aaggaagaaa
     1081 atatcaggta aagcagcctt taggctcggc tcctatggat ggtccggagg ggctgaaaaa
     1141 gagctgaagg aaataatcga gagaaacaat atgaaatggg atttcgtaga gacagttgaa
     1201 tttgaggggt cgcccaaagc agaagattta aataaaatag aatccagagt tttggaactg
     1261 attgcaaaaa tgaaggataa gatagttgaa tagtagtgag ctaatagctc cggataattg
     1321 ctccttatcc taatcaaaca ggtcaaaata taatgaggga tattcatatc tctgcattta
     1381 aaacttttta acagtaaatg aatgaacaaa caaagccctc cataatgata ctggggggct
     1441 tattgcaata attattcaaa agtctttata taaggtaata tagagatatt tgtcgaagat
     1501 atattttcgc aataccccca aatatttaac gggggtaacg aaaactataa tatcaaagat
     1561 tttgaaacaa aatttgaatg gaggaaatag aatgtacaca aaatatttta aaagaattat
     1621 tgcttttgta atcgtattgt ccatggtatt tacatcagtt gctatggcaa tgccttacgg
     1681 gcaggcaaaa aagtttatca gaaacggata cagctgggag aaagttcaaa agtatgtaca
     1741 gggaaagggc ataatgaagg gatttcccga cggcaagttc tatgagaatc tttatgtgaa
     1801 acgtgccgat gcaattgtta tgattgacag agcatttaaa ttaaatgctt tgatagattc
     1861 actaaatatt gattatgaag aattatttga tgatgttgaa gttggcgaat attattttca
     1921 ttcaatatac atagctagag ctttaggtat aaccaaaggc ataggaaaca ataagttcaa
     1981 cccgaaacat tccgtcacta ttgaagaagt tatacttttg atagagaggg caatgggaaa
     2041 aaacaaatac ttcgaatttg atgaggatat tgatttaagg gaggattata aagagctggt
     2101 tggtaaaggt ccgaatggaa agactaaaga tttgaaagat tttgccacca ggggcgaaat
     2161 agctgccatg ctttactatg ttctgacagg aagtaaatat gatggcgaac ctgtagatga
     2221 agaagaaaat gaagataaaa taacacgtac aatgaaagaa aatgaagtgt taaaatttga
     2281 tgttaaatat gataataaag acagcttgat agagaatatt gaagacctta ttgatgatga
     2341 tttagactat gttaagttca caaagccttt aaataaagac aacagctttt tatattatga
     2401 ttatgatgag gatgacagtt cacactcatt tgtaatcaaa ggtaaagagt attatgttaa
     2461 aacaatcgga aaacagatgg acctgtcaga agtaaccata gtacctaaaa caacaggtgt
     2521 gttaaaaata gaattcacag catatgataa agacgataaa ccatatgatg gtataatcga
     2581 aatcactgtt gaacctatgc ttggagaatt gctaattgat gaaaatgata ttgatgattt
     2641 tgccgaagaa gttaaaggct atttagaaga atttgatgaa tacgattttg aggagattat
     2701 tttcgagaaa ctcgacgaag atgatgatta tgatttaaaa attgaaggta ggataaggtc
     2761 tcttttcggg agaaatatat cttttgagag gctgggaaaa gtaaagttta ttgacggtga
     2821 tgattttgac ggtacaacat cattaaaata tacggttaca acagatgatg gattgaaatt
     2881 taccggatta attgaaattg acgattaaat aataagaaaa tacagacaaa ggactgagct
     2941 tgacaatcaa actcagtcct tttttcaagt ttacatttga agaataaatt agattaatat
     3001 atagtaattg gaaaaactat ttcttttggg ataattaaaa tatttttatt gttgatttaa
     3061 atcaatttat tattttaaaa tatttggtag aatataaaca ttaagaataa gaaattaatg
     3121 aaagaaggag acaaaatggc aatatttaat gaaacagtta aaaggctgat accgacattg
     3181 gaaaaattcg tacctattgt tgacagggtt catggtgaac accacccgga attccatgat
     3241 gtccgtaaaa tatttaatac aataaatgaa aaggcaaaag aagcaggaac agaaaaaccg
     3301 gatttaagcc gggaatttgc caagctgcgt gaaataacag ataattacac cgttcccggt
     3361 gatgtatgcg aaagctatga agcagcctac aaaatgctgt ctgaattgga tgaatcctat
     3421 caggcctaaa acctaatctc tcaaataact ttgagggatt tttttaaatt gacaaaggta
     3481 ttttcaataa agattttcat tgattgttat aaacataaat tgtattatta aaatataaaa
     3541 tgctacattt caaggaggat ggaatgaact atttacagct ggatacgccg gctttattga
     3601 ttaataaggg tataatgctg gataatataa aacatatgca ggattacgca gatgaaaaaa
     3661 atgttgcact gagacctcac accaagaccc acaaaatgtc taaaattgct ctattgcagg
     3721 aagaagcagg ggcaaaagga atcacggtgg caaaggtcgg tgaggcggaa gtaatggctg
     3781 caaacggatt aaaggatatt tttatcgcca atgagattgt tggcgatata aaactttccc
     3841 gcattaaaaa gctggctgaa acaatagata tatcctttgg acttgacagc atagctcagg
     3901 cagctatggt tgagaaagca tttgaaggaa gcaaaaaagc aaatgtatta attgaaattg
     3961 aagtgggtga aaaccggtca ggcgtcattg aagaggctga ttatatagag cttttgaatt
     4021 atttgaaaaa atgcagcaat attaacctga agggagtttt ttctcatgac ggtcattctt
     4081 attcagctga aaacattgaa gcttgtaaaa aaattcactt ggaagctcaa agaagaaccc
     4141 tgcgttttgc agatattgca aacgagatgg gatttaaatt agaaacagtg agcatcggct
     4201 caactccatc cttgttgcat aatttcccta tattggacgg tgttacggaa atcagaccgg
     4261 gcacatatat tttcatggat gcttcccagg ccgctgcata cggaagtttt aaaagaaatg
     4321 cagctacaat cctgactact gttataagcc ttcctacacc tgaaagggta ataactgatg
     4381 tgggtgccaa aggaattaca atgcaggcta ggaacaaggg gttcactgca acaaaaggtc
     4441 tgggtcttat aaaaggatgg cctcaagtta atatttatgc tgtatatgat gagcatgcaa
     4501 taatatataa caaggaactt catgattcca taagagtggg agataagctg gagataatcc
     4561 ccaaccacat ttgtcctgtg gtaaatcttt atgaaaccgc ctatattatt gaaaatgacc
     4621 gggtggtaga tgagataccg gttgattgca gaggaaagct taaataaaga aaaaacctct
     4681 gcgtcgttct taaggtgcag cttgaataag gggatgaact aaatgttcat cccttgatat
     4741 attatttatg aatatggctt atgatttttt ctttcatgtg attaatgaaa tcttcatggg
     4801 gtttttccac aaagaacatg gaaagacctc ctaagcccac gtgggtacca acagccaccc
     4861 ccatctgcat aatataaacc tgacccttaa atccggcttc aataagcaga gcctttaaat
     4921 tctccgcttt attaatgtcg gatgtatatc cgacgataac aaaattagtc tgctcgtaat
     4981 ctacccgatt aataaactct tgaacgtaat aattataaac ctgtttactg cctctctttt
     5041 tggcaacaac agcgcctttt gtattcttca ttgacataat cggctttaca ttcagcaatt
     5101 tgcctattac accgcttgca ttcgacagcc ttccgctgcg tatcagacta tccaaatcat
     5161 ctaccgataa aaaatgcttt attctgtatt ttattgtttc acagtattca acaagctctt
     5221 caaatgtata tccttcttca ataagcatgg cacatttcat aataagccat ccgcttccat
     5281 ggctcatgga aaccgaatct attacttgaa attcgatatt ggaatcagga ttttgttcaa
     5341 aaaataattc cttggccagc aaggcacttt ggtaagaccc gcttgttccg cttgacatgc
     5401 atatgcaaag aaccttttca tacccctggt ctattgctcc cttaatacat tcaatatatg
     5461 attcggggct tggcatcgaa gtggttggat atgttttaaa ggtcatcagg ttcttataaa
     5521 attcatcgtt tgaaatatct atgcggtcct tgtattcctt tccttcaatt gtaacaacca
     5581 atggagctaa gcaaatattg taacgattta atatttcttg tgataaatca catgttgagt
     5641 cagcaattaa ttttgttttc atacaagctc ctattcttat acagtattta gctacgctag
     5701 tataagattt taatataata tcatcctatt cgacaaaatt caacaatttt ctacaaccct
     5761 gcatgaacaa gcaggcctaa aaattattaa gcaatataat ttcaaaaaca taaccttgat
     5821 ttaactcagt aatactttta atatagacgt aaacaaggat tagcagcctt gttttatatt
     5881 actttacgta atattaataa tataagaaca ggcaactgaa aagggggtta tggggattga
     5941 ataattatat taaaggaatt aaaaataagt atgtgataaa caaggttcaa aacacaagga
     6001 ttcctgattt caaaccatcc aaaatatcaa gaagtctgat tattttttcc ggcagggtcc
     6061 aaaaagtcgg ctttagacta gaaatatatg aattggcaaa acggcttaat ttaaccggaa
     6121 gtgtaagcaa tatgaaaaat ggggatgtca ggcttgaaat tcagggtgaa aaggaaaaaa
     6181 ttcttttttt gactggacat atgaggtcat tgaaaagagc aaaggtaaga aatgttagat
     6241 ttcaagatga ggttttggta aacggtgaat caagttttat ttatgaacct taatgtgcca
     6301 ggatgttcac acttatatgg attaagtcac cgattaaata gagccagtca gattgtttat
     6361 tatctacact ggcttttatt aatcctgtaa cttatctgcc aatcagaaaa agagcaaaat
     6421 atcaatatta gtcttttctt taaagtctca ccaaacattt ttaagtaagt tataaatcac
     6481 actttaaagc attatacaat ttcaaaaaca gtgccttgat ttagatcagt aacattggaa
     6541 gagccgtaga cgcctaaata cagttttgat tgagtcagat ttgttcccaa gcttacatag
     6601 taagccgatt gggacccgaa attataatta gtatcaataa ggctgtaatc atactgcctg
     6661 cattccgttc ttacctttgt ataggctaat gcccctctta caggggattc tcctttcctg
     6721 gcaaaatcga taaatacaat acagcctgtc agatcgggaa tatcatagcc catatagggc
     6781 tgaacacctg tgagtgcagt acctctgaat ttatcgggac gtgaatccat gtgatagtag
     6841 caagtcagcg gcagcaggcg cttagctgca gtacttattg cttcattata ataagctgtt
     6901 gttttctcaa ccagcgatga atttgacagg cagctgttta taattggcgt aggcaaatca
     6961 ccttcccatc ctctccagcc aaggttgaca aaaccttcct gatcagagct ttggtttgct
     7021 gcttgagtaa tttctgtcac cggtatgggc ttataggaag taaaagtaaa aatcgactca
     7081 atcaaatcct ggcctacatt ccccgcatac tttatatatt gattataata cctttgaaat
     7141 gctatgcctg gaatattgcg gacgcctttt gccattacgg taagtgttct ttgaacagct
     7201 tcgggaagct cgttaaatcg tgtaacaacc ggcagattgt ttatggctgt atcagtattt
     7261 acatctattt caattatttt gccggcaacc tccataatat tctgcgctga attaaaggga
     7321 tcatatcctg aacccccgtc tccggtagta agtaccagcc ttcctgtttc aggtgaaaag
     7381 tttaagctgt tgacaccgtt atggttcatg aatggccttc ttaagttcag caaggtacgc
     7441 cttttttgag cttgaccgtt ggactgcagg gaccattctt ccacagtatc tatatggtca
     7501 tattgggtat ctctatttgt ccatctcaaa ttcaatgttt cagggtcgca gggatttggt
     7561 tgaaaagacc ctgaaagagc acctgggcct tgtgttcctg ctgctgaata atgaaggtaa
     7621 aaaagaccgt taagataaaa attaggatga aaggccagac ccagcagacc acgttcatcg
     7681 tagccgccgt tttggcccag acggataatc ctctggcgga tatccaaaaa aagccttatc
     7741 tgtccatttc tgatattata gatttctcca acctggccgg caataaatat actctcatat
     7801 gaatctcccg gaagtactgc tgtttttata acggtgggca aatttatatt gctgacaagg
     7861 ggacgcagac taacactaat ctttttcaat tttattcact ccttttattg ttttgagtgc
     7921 atatctcttc ctagtttaaa tatatgtttg aagatatttt attagtacag gaggattagg
     7981 ctattacgtc ctttcattat ttttcaatct ttagtacaat atattaagta agaaatttta
     8041 gaacacagga atatatattt gtttatacgc aaatttgttt gcaacctttt acttatttta
     8101 tcgtcaaaag ggcaaggaga gtgttatgac agcggaaaaa ttattgatta aaaacttaaa
     8161 aagaggccgt gaagaggcat acagacaatt aatcgaagaa tacggcaata gactgctgag
     8221 aacctgctgt ctgatactga aagacaggga ggaagcagag gatgttgtcc aagaaacatt
     8281 tataaaggta tttaataaaa tagacacatt taaagaaaaa tcaggactat acacatggat
     8341 ttattcaatt gctctcaatc tgtcaagaga cagaatgaga ttgaagcagg atatgcttga
     8401 gttgaaagat gagtggatag gaaatgatga tgtggaatct caggtagaaa taaatattga
     8461 cagagagctt ttaagaaaag agatatttga aatgaattca ctctacaaag aggtgcttgt
     8521 gctttactac tatgaaggcc tctccataaa agaaatatca tatctgctta atgaaaagga
     8581 aggaacaatt aaaagcaaac ttttcagggg gagaaacata ttgaaggaag gccttttgaa
     8641 aggaggtcag aaatgaataa gaaagataag caagataagc ttggaatgga aataaaagat
     8701 attatggaag aagaaacctt tgacctgacg ctttctcaaa aagcctttga taatattatt
     8761 aaacatagga agaaatccag gaatgaaaaa attcgggagt ttttgaacag agatattgaa
     8821 atacccctgg caccagcaat tgtcggttta gctgccctgt ttgccatcac attgattccg
     8881 ggcaatgtat ttaagtctca aaatgaagaa attatcaata tgggaaactc tcaagtaata
     8941 atcagggaag attttgaggt gagccaaaaa tgaaaataaa gataaaaata aagacactga
     9001 tactaactgt atttgtttta gcattaacat ttatattggc catacctaca gcgaccctca
     9061 tgattgcagg ttttcttgat gatagaggat cagaaaaggc gtctcttttc tacgaaaggt
     9121 atgcttcata cgccaccaca ccaaatatta agagtaatta tctttatgca gacagcctag
     9181 taaagagttt taccaagtat acaatattct ttaccggctg gggcggaggt gcaaatacaa
     9241 gccctgaaga tatggataag gcaaagcaga ttcttataga aacattagcc gaaacacccg
     9301 ttaaaaatag tgaaaaagac tactatataa aatcatacaa aatgcttctt gatatagcta
     9361 tagcaaccgg ggatgcaaga atgctccgtg aatggattga tttcggccag gaggtcgaag
     9421 atgaaaaatt aatatatact gctgacatat atgaagggtt tctccaccat gtaaatggag
     9481 aaagtgaagc agcaaaaaga gtacttgaaa aatatgaaga tacggagtca aaagacataa
     9541 gttatgatgt attaaaagca gagattgcct tgtttgacgg taaatatgaa gaagttgaaa
     9601 ccatatatga aaatttatat aaaaataatt ggagagaatt aggaagaagc ttcggctcag
     9661 gaggctacga cagaggtcat tggtttgaag aaagattcga tgaccttaag ggtgatagta
     9721 ttcttagagg aacagtaacc tatgaaggaa agcccatgcc ttttgtagaa atatatgtcc
     9781 aggaagcaac cggtggttta agcttattaa gccgcggaga aagctatgca ggcataactg
     9841 atgaaaacgg tcaattccgg acattaggtc tcaaggaagg attgtacaat gtgggaattg
     9901 gggtagatgg ttccatcttg acggacaagg agcttcaaag ctcaacaaat aaatatgttg
     9961 agctggacga aaagggggga gaaattcact ttatattcag agacacccta aaaataaact
    10021 cgcctaagct cggagaaaaa ctatcagggg atgaattcac tgtatcatgg gaggaagtag
    10081 aaaatgcagc ctattatacg gttggagctg tggtttttta tgagcccttt ggcgataagg
    10141 gtatcagcac gcaaacatat ttaaaagatt tgaatggaga atataaattt actgaaacta
    10201 atgctgtttt taatataaat ttgtttagaa ataaccctaa tatgaatgtt tctgattcag
    10261 aaaaagagta tcttggtgct caagcagttc ttggaatatt ccttccaggt gttgagtatc
    10321 caattgttgt aaatgcattt gatgaaaaca ataatctcat aacaagaagt cttcctaaaa
    10381 tatcatatta tgaccagata ccaggcatta cagttgaagg aagtttaact gaaggagaga
    10441 acttgattta tcataaaaat tatattgaag ctcttgaata ctacgaaaat attctcacag
    10501 agaatcccga tgacacagct gccctaagat accttacaag aatatacggc ataggctgga
    10561 aagaagggga gcaaaatctc aaaagggcat ttgaattagg gcagagatac tcagaaattt
    10621 caggagattg gaatttgtta atcaatacgg ttgctattat ggatatgagt gaaattaaag
    10681 aaaacagtaa ccgtgttttc gaagttctta cagaagctat gaaggatcca aatttcgata
    10741 actattcttt tctaagcaga tattatattg ccgttgaaga ctatgaaaag gcaagagaaa
    10801 cactgaggga atgggaaact gtaacagata cactaatttt cctgaacata tattttggag
    10861 attatatgga agcttcaaaa aatattaaat cggaaagctt ctatatatca aggctgtcat
    10921 caaatagagt taaaggggca cttgagg
//
LOCUS       DAOPUT010000052        10133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_6685_length_10133_cov_19.410002, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000052 DAOPUT010000000
VERSION     DAOPUT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6685_length_10133_cov_19.410002"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1201)
                     /locus_tag="PKK75_05685"
     CDS             complement(53..1201)
                     /locus_tag="PKK75_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNZ83125.1"
                     /translation="MQRDCEKERQQIERYRSILKTSFPVLITALIILLIVFSPGTFSW
                     IFGSFKPVIYGLVIAYLLDSEVVFFMKKLKVRRKQGIFLACIILIGILSAAVYKVLPQ
                     IIDNVNNIMSFVMGGEVDIAQIVSDIRSRVDNKYVDYVTGYILQAGESVRNIINNLLR
                     YLYNTLMSLITNIGSGTFTAVTSFIIGIYMMSEKDDLLARGRRFIHAYFKENKANDIL
                     RVFGRANKIFKSFLIGKLLDSTIVGIICIIAFAIARVPYATLMGTLIGFFNIIPYFGP
                     IIGSVPVVVVSFFVNPAKALTAMIIIIIIQQIDGNFLDPKIVGENVGVSPFWIITSVT
                     VGGNLFGIPGMILGVPVVVLLKEVLEGSIEMRLIEKGKENIEKSNLRK"
     gene            complement(1300..2736)
                     /gene="pyk"
                     /locus_tag="PKK75_05690"
                     /pseudo
     CDS             complement(1300..2736)
                     /gene="pyk"
                     /locus_tag="PKK75_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
     gene            complement(2795..>3361)
                     /locus_tag="PKK75_05695"
     CDS             complement(2795..>3361)
                     /locus_tag="PKK75_05695"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ83126.1"
                     /translation="YTKYTLGFFTALTTVLESVEKIRDTSSSHNRSNVIEVMGRNCGD
                     IALYAGLAGGAELVIIPEIEYNIDEISDRVMQGRTRGKKHSIIILSEGAGDAREISEQ
                     IEKKTGITTKYSILGYTQRGGAPSAYDRLVGSQMGAHAVDLLCSGKRNRAVGINGSGI
                     FDMDLDEALAVEKKFDETIYELTKILAI"
     assembly_gap    3362..3461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3462..3631)
                     /locus_tag="PKK75_05700"
     CDS             complement(<3462..3631)
                     /locus_tag="PKK75_05700"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ83127.1"
                     /translation="MRKIGVLTSGGDAPGMNAAIRAVVRYGIYNNIKVTGIYNGYQGL
                     VEGDHMEMNLSSV"
     gene            complement(3649..7167)
                     /locus_tag="PKK75_05705"
     CDS             complement(3649..7167)
                     /locus_tag="PKK75_05705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNZ83128.1"
                     /translation="MSDNKFTHLHLHTEYSLLDGACRIKELIKKAKELNMDALAITDH
                     GSMYGAVEFYKQAKKEGIKPILGFEAYISPRRMTDRDPQVDKNLYHLVLLAENQQGWQ
                     NLMTLCSLGFVDGYYYKPRIDHETLRVYKDGIIALSACLAGEVQAYLLEDNYNEALKA
                     ALLYKDIFGEENFFLEMQDHGINEQKAVNDYLQKISRETGIGLVATNDVHYINKEDAY
                     FHDVLLCIQMQKTIMDEDRMKFPSDEFYLKSFEEMAGLFPAAAIGNTARIAERCNVDL
                     DFNTVHLPEFKVPEGYEKSEYFRKICQEGLRERYKNITVEIRQRLEYEISVIEQMGYV
                     DYFLIVWDFIKYARDNKIMVGPGRGSAAGSLVAYSMKITNIDPLKYNLIFERFLNPDR
                     ISMPDIDVDFCYERREEVIDYVKEKYGHERVAQIITFGTMAARGSIRDVGRALNMPYG
                     EVDFIAKKIPMAIGMTINSALEVNKELKDLYEGETKVKKLIDLAMQVEGLPRHSSTHA
                     AGVLISKEEVTKYVPLSRNKDVITTQFNMIELEELGLLKMDFLGLRTLTVIRDAIDLI
                     EQNHHAKIDFQNCNYDDKKVYKLFANSETLGIFQFESSGMRAFLSELKPSEFENLSAA
                     NALYRPGPMGQIPVYIKNKLNPDKVTYIHPKLEPILNVTYSCMVYQEQVMQIVRDIGG
                     FTMGRSDLLRRAMGKKKMDVMLKERQNFIYGKKAENGEPEIRGAVNNGVDEKTANEIY
                     DLMAEFAKYAFPKAHSVAYAALAYETAWLKTYYPVEFMAALISSIMGSAGTVSLYVRE
                     CKRLGIEILPPDINESMGKFTVENRSIRFGLAAVKNVGTNVIQEIVRAREENGRFTSF
                     ADFCTKVDPVVLNKRQIESLIKCGAFDSLNVRRSQLMAIYERTIDSVTAQKKRNIEGQ
                     FSLFDSFKTEVEICDELPLLPEYNEKTLLSMEKEMVGLYLSGHPLSEYEGVLEKYATT
                     NSSEISEISEQQETVLYDGSRVVLGGIIIKKQNKITKNNNMMAFITLEDLYGTVEGII
                     FPKIYDNCKDILYEDNIILVEGTIDASEEETPKLIINKVTELKKEHEELTGQNNQNNQ
                     NTQNNQNKQNKLYIKVKDTENYKNIKKDLFHCICSHKGGDCVIIYNEKDKANMVLPNK
                     YSVNAEDEVLITGLKELLGEKNIAII"
     gene            complement(7251..7823)
                     /locus_tag="PKK75_05710"
     CDS             complement(7251..7823)
                     /locus_tag="PKK75_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ83129.1"
                     /translation="MIYLEIFVSFFYIGLFSLGGGYAALPLIEGQVLDVKNWLTVAEF
                     ADLLTISQMTPGPIAINAATFVGTKVAGLPGAVIATIGCVTPSFIIVLTLSYFYFKYK
                     RLSSIQGILKGLRPAVVSLIASAGLSILLLAIFKTEGTSMLNMKLSDINFIGIIMVAI
                     SAFILRRFKPDPIKVMLGTGIAGVIIYGFL"
     gene            complement(7820..8389)
                     /locus_tag="PKK75_05715"
     CDS             complement(7820..8389)
                     /locus_tag="PKK75_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ83130.1"
                     /translation="MGRNINFYRQLFLSTFTISAFTIGGGYVIVPLMRKKFVEELKWI
                     DEEEMLNIVAISQSAPGPIAVNTSIMVGYKLAGIPGALITTFGTILPPLIIITIIAKF
                     YEAFKENSIVSALLLGMRAGVAAVIIDVIIKMTKDIIKSKNNVSIAIMVIALIATVIF
                     DINAALIIIISGAFGGFYYNFVRKAGESQ"
     gene            complement(8448..9980)
                     /gene="cls"
                     /locus_tag="PKK75_05720"
     CDS             complement(8448..9980)
                     /gene="cls"
                     /locus_tag="PKK75_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNZ83131.1"
                     /translation="MEKFISFLLSRFTLVSLALLAQIITLALMVYRFSSYFLLFDIVF
                     TVISVLAVLYILNRKSDPAYKIAWIIPIMLFPVFGGLFYLMFGGTGLSNRMENNMHTI
                     VDKMKECLHQHPVTLYNLNKENKIAFNQAIYIERYSSCPVYDNSATEFLPSGEEFFEH
                     LTRELKKAEKYIFMEFFIIEEGLMWNTILGILKEKAEQGLDVRIVYDDFGCVRRLPHN
                     YYKILEGYGIKCSVFNPYIPVLSFRMNNRNHRKIVVIDGKVAYTGGVNLADEYINVVK
                     KFGHWRDSCIEIKGDAVWSFTVMFLSMWGYLRKNNEEYDKFRPDDYDQSQECHGYVQP
                     FHDSPLDSEHVSETVYLNLINQAEKYIYIETPYLIIDNKMIVALTMAAKRGVDVRILT
                     PFIPDKWYAFAVTQSNYGELMEAGVSIYQYTPGFNHGKTFVVDDEYAVVGTINMDFRS
                     LYLHFECGVWLYNSASVWQVKESTLEALRVSSQVEMEDITNIPKYKQIKRLLLRIFAP
                     LM"
BASE COUNT         2688 a         2219 c         1663 g         3463 t
ORIGIN      
        1 attatattac acttgattat atttgtcaat aaaaacttta taaaaaaacc tcctatttgc
       61 ggaggtttga tttttctata ttttctttgc ctttttcaat aaggcgcatt tctatggagc
      121 cttctaaaac ttccttcagg agaaccacaa ccggaacacc taaaatcatt cccggaatac
      181 caaaaaggtt tcctcccacg gttacgctgg taataatcca aaaggggctt accccaacat
      241 tttcacctac tattttaggg tccaggaaat ttccgtctat ctgctgtatg ataattatta
      301 ttatcatagc agtcaaagcc ttggcagggt ttacaaaaaa tgacaccacg actaccggaa
      361 ccgaaccgat tatgggaccg aaataaggta ttatgttgaa aaatcctatc aatgttccca
      421 taagagtagc gtagggcacc cttgcaatgg caaatgctat tatgcatatt attcctacta
      481 tggttgaatc caagagcttg ccgattaaaa aacttttaaa tatcttattt gctctgccga
      541 atacccgaag tatgtcattt gccttatttt ctttgaagta agcgtgtatg aatcttcttc
      601 ctcttgccag caagtcatct ttttccgaca tcatgtatat gcctattata aaactggtaa
      661 cagcggtaaa tgtgcctgag ccgatatttg tgatcaatga catcaaagtg ttgtacaggt
      721 atcttaaaag gttgtttata atatttctga ctgactcgcc tgcctgtaaa atgtaaccgg
      781 ttacataatc cacatacttg ttgtctaccc tgcttctaat gtcactcact atctgggcta
      841 tgtcaacttc accacccatt acaaaggaca ttatattatt cacattatct ataatctggg
      901 gcaggacttt gtatactgca gcgcttaaaa ttccaatcaa aattatgcat gcaagaaaaa
      961 taccctgttt tcttctgacc ttgagttttt tcataaaaaa cacgacctca gaatccagca
     1021 ggtatgcaat tacaagaccg tatattaccg gtttaaagga accaaaaatc cagctgaatg
     1081 tgccggggct gaatactatc aataaaataa tcagcgctgt tatcaatacc ggaaatgaag
     1141 tttttagaat acttctatac ctttctattt gctgcctctc tttctcgcag tctcgttgca
     1201 aagtcttatt tgctccttac tccgcataaa attgacactc actgcgaatg acctgccagc
     1261 catttctaag aaaaatgact ctgagaaatg gggttagggc atctcctcgc ccacaatgtg
     1321 aacctttaat aagtttgtta tgccggaaat tcccgccgga actcctgcag ttattattgt
     1381 taagtcgcct tttttcaaat atcctgattt tagtgctgtc tcgacagact tttcaattat
     1441 caggtctgta gactgcagtt tttcaatttt tatgggatat accccccaga aaatggctaa
     1501 ttttctcata acctgctcga atggagtggc agcaattatg ggggctgcag gcctgtatct
     1561 cgagacactt gctgctgtat agcctgatgt tgttgcggat attatagcag atgcacgtaa
     1621 atccaaggat gatttgcagg ttgcatagct tatggaatca gttacactgc agttggaagc
     1681 ggggcttttg cttttcaaaa gttcgttgta gtttaatgag ccttcaatca taagagctat
     1741 tcctgccatt gtttttaccg cttcaaccgg atagcttcct gaagctgttt ctcccgacag
     1801 cattattgca tcggtacctt caaatattgc agtagccaca tctgaaacct cggctctggt
     1861 aggccgcgga tttcttatca ttgaatcaag catttgagtt gctgttataa caggttttcc
     1921 tgcatcattg cattttctaa ttatcatctt ttgcactaaa ggaacttctt ctgccggtat
     1981 ttcaactccc aagtctcccc gggctaccat tattccgtca gaaacttcga ttatttcatc
     2041 aatattttca accccctgcc tgttttctat ttttgaaata atcattatgt gccctgcatt
     2101 ttcttcttct aaaactcttc ttatttctat tacatcattt gccttcctga tgaatgatgc
     2161 tgcaatatag tcgattccca gctcaatacc gaattttata tcgctaatat cgccgtctgt
     2221 cagtgcaggc agattaattt gaacatttgg aacatttatg ccttttttgt tttttacagt
     2281 cccgccgttt ttcaccaggc atttaacatc ggtattattt ataatttcaa ctgcttccat
     2341 gctgataagt ccgtcatcta taagaattgt atcacctttt tttatgtcat ttgcaaattg
     2401 actgtaggtc acgctgacaa ttttatcatt ccccgttatt tctctggttg tcagaataaa
     2461 atcctgccct tttaccagtt cagcctgacc ggtttcaaaa tctcttaccc gtatttcagg
     2521 tccttttgta tcaagcatta ttgctgtttg agtattcagt tcttctctta cttttttgat
     2581 tgtaataatt ttttctttgt gttctgcatg tgtaccgtgg gaaaagttca atcttgccac
     2641 atttaagccg ttaagaatca gctccttgaa tatttcttca tcctgggatg aaggtccgat
     2701 ggtgcacact atttttgttt tcctcatatt taacatatta gctcctctcg ctgaaggtgt
     2761 gtccccacac ccgcagtctc gctgcacaat tttattatat cgccaatatc tttgtcagct
     2821 catatattgt ttcatcaaat ttcttttcaa cggcaagagc ttcatccagg tccatgtcaa
     2881 atattccgct tccatttatt ccaacagccc tgtttctttt gccgctgcat aataaatcta
     2941 ctgcatgagc tcccatctgg cttcccacca gcctgtcata ggctgagggt gctccgcccc
     3001 tctgggtgta tccaaggatg gaatactttg tggttattcc tgtttttttc tctatctgct
     3061 cggatatttc ccttgcatct ccggcacctt cggataaaat aataatgctg tgttttttgc
     3121 ctcttgttct tccctgcatt actctatcgc ttatttcatc tatattatat tcaatttcag
     3181 gtataataac caattcagcc cctcctgcaa ggcctgcata aagtgcaata tctccgcagt
     3241 ttcttcccat aacctctata acatttgacc tgttgtggga tgatgatgta tctcttattt
     3301 tttcaactga ttccagcaca gtagtaagag cagtgaaaaa gcccagagta tattttgtgt
     3361 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacagaagaa agattcattt
     3481 ccatatggtc tccttctacc aagccctggt agccgttgta aattcctgtt acttttatat
     3541 tattataaat cccgtatctt acaaccgctc ttatggctgc attcatgccc ggcgcatcgc
     3601 cgccgcttgt taaaactcct atttttctca ttttaccctc cgatccaact atataattgc
     3661 tatatttttt tctccaagca attctttcag cccagtaatt aagacttcat cttctgcatt
     3721 cacactgtat ttattgggca gaaccatatt agccttatct ttttcgttgt aaattataac
     3781 gcagtctccg cccttgtggc tgcatatgca atgaaacaaa tcctttttga tatttttata
     3841 gttctctgta tcctttactt ttatataaag tttattctgc ttattctgat tgttttgggt
     3901 gttttgatta ttttgattat tttgtcccgt taattcttca tgctcttttt tcagttcagt
     3961 cactttattt ataattagtt ttggtgtttc ttcctctgaa gcatctattg ttccttcaac
     4021 caggattata ttgtcttcat ataaaatgtc cttacagtta tcgtaaattt taggaaaaat
     4081 tattccttcc acggttccgt acaagtcttc cagggttatg aatgccatca tattgttatt
     4141 cttcgttatt ttattttgtt ttttaataat tatcccgcct aaaacaaccc tgcttccgtc
     4201 atacagaact gtttcctgtt gttcacttat ttcggatatt tcactggaat ttgttgttgc
     4261 atacttttcc agcactccct catattcgga aagaggatgt ccgctcaagt ataggcccac
     4321 catttccttt tccattgata atagtgtctt ttcattgtat tcgggcagca gcggaagttc
     4381 gtcacaaatc tcaacttctg ttttaaagga atcaaagaga gaaaactgtc cttcaatatt
     4441 tcttttcttt tgagctgtta cagaatcaat agttctttcg taaatcgcca taagctgcga
     4501 acgtcttaca tttaaagagt caaaggcacc gcattttatc aatgattcaa tctgcctttt
     4561 gtttaataca accgggtcaa cctttgtaca gaaatctgca aagcttgtaa acctgccatt
     4621 ttcttctctt gcccttacta tttcctgaat tacatttgtt cctacatttt ttacagcagc
     4681 aaggccaaag cgtatgctcc tattttctac cgtaaatttt cccatacttt catttatatc
     4741 aggaggcagt atttcaattc caagccgttt gcactccctg acatagagtg acacggtgcc
     4801 tgcgcttccc ataatgcttg atattaaagc tgccataaat tcaacaggat aataggtttt
     4861 aagccatgca gtttcatatg caagagcggc gtaagctacg gaatgggcct ttgggaatgc
     4921 atattttgca aactcagcca tcaggtcgta tatttcattt gctgtttttt catcaacacc
     4981 gttattaaca gcacctctaa tttccggctc accattttcc gctttcttgc cgtaaatgaa
     5041 gttctgcctt tccttgagca taacatccat ttttttcttg cccatggcac gtctcaacaa
     5101 gtcggaccga cccattgtaa atccgccaat gtctcttact atctgcataa cctgttcctg
     5161 atacaccata cagctgtagg ttacgttaag tattggctca agctttgggt gaatataagt
     5221 aactttatca ggatttaact tgtttttaat ataaacgggt atctgtccca ttggcccggg
     5281 tctgtatagc gcattggcag ctgaaaggtt ttcaaattca gagggcttaa gctcgcttaa
     5341 aaatgctctc atacctgagc tttcaaactg gaatataccc agtgtttctg aatttgcaaa
     5401 aagtttgtaa acctttttgt catcataatt gcagttttgg aaatctatct ttgcatggtg
     5461 gttttgttca attaaatcta tagcatcacg aataaccgtg agagttctta atcctaaaaa
     5521 gtccattttc aacagtccca gttcttcaag ctctatcatg ttgaactggg ttgttataac
     5581 atccttgttt cttgaaagag gaacatactt tgttacctct tcctttgata tcaggacacc
     5641 tgcggcatga gttgaagaat gccttggaag tccctccacc tgcatagcca aatcaatcag
     5701 ttttttaacc tttgtttcgc cctcatacag gtccttaagc tccttattca cctccagagc
     5761 agaatttatt gtcattccta tggccatagg gattttttta gcaatgaaat ccacttcgcc
     5821 gtagggcatg tttagtgccc ttcccacatc tcttatggac cctcttgcag ccattgtacc
     5881 gaatgttatt atctgggcta ctctttcatg gccatacttt tctttaacat agtctataac
     5941 ctcttcacgg cgctcgtagc aaaaatccac gtcaatatcc ggcatgctta tacggtccgg
     6001 atttaaaaat ctttcaaaga tcaaattgta tttcaagggg tcaatatttg taattttcat
     6061 ggaatatgca accagacttc ctgcagcaga ccctcttccc ggtcccacca taattttgtt
     6121 gtcccttgca tatttgataa aatcccacac tatgagaaaa taatccacat aacccatctg
     6181 ctctattacg ctgatttcat attccagtct ttgccggatt tctaccgtaa tatttttata
     6241 cctttccctt aatccctctt gacatatttt cctgaaatat tctgattttt catagccttc
     6301 aggcactttg aactctggca ggtggacagt gttaaaatca aggtctacat tgcatctttc
     6361 tgcaattcga gctgtatttc ctatggctgc tgcaggaaaa agccctgcca tttcctcaaa
     6421 ggatttgaga taaaactcat cagacggaaa cttcatccgg tcttcatcca ttatagtttt
     6481 ttgcatttga atgcagagaa gaacatcatg aaaataagcg tcttccttgt ttatatagtg
     6541 aacatcattt gtcgcaacca gccctattcc tgtttccctg cttatcttct gcaagtaatc
     6601 attcacagct ttttgctcat ttattccatg gtcctgcatt tcaagaaaaa agttctcttc
     6661 tccgaatata tccttataca ataaagcagc cttaagagct tcattataat tatcttccaa
     6721 cagatatgcc tgcacctctc cagccaagca ggcgctcagt gcaattattc cgtccttgta
     6781 gaccctcaat gtttcatggt caattcttgg tttataatag taaccatcta caaatcccag
     6841 ggagcaaagg gtcattaaat tttgccagcc ctgctgattc tctgccagga gaactaaatg
     6901 gtagagattt ttgtccactt ggggatccct gtcagtcatt ctgcgggggg atatatatgc
     6961 ctcaaaacca agtataggct taattccttc cttttttgcc tgtttataga actcaactgc
     7021 tccgtacatg ctgccatggt cggttattgc cagggcatcc atgttcagct cttttgcctt
     7081 tttaatcagt tctttaattc tgcaggctcc gtcaagaaga ctgtattccg tatgcaaatg
     7141 caggtgtgta aatttgttgt cagacatttt ttctccttaa ttttgagacg gttattttct
     7201 ggggacggtt atttttgttc tcattagcat ccccgtcccc ttggcatgtc ttatagaaat
     7261 ccataaataa taaccccggc tattcctgtt ccaagcatta cctttatggg gtcaggttta
     7321 aaccttctaa gtataaaagc gcttattgcc accattatta ttccaataaa attaatatca
     7381 ctcaatttca tatttaacat ggatgttcct tctgttttaa atattgccag cagcagaatg
     7441 gacaggcctg ctgatgctat cagtgatacc actgccggtc ttagaccttt caatattccc
     7501 tgaattgatg acagtctttt atatttgaag taaaagtaag acaaggtcaa tactattatg
     7561 aaggaaggtg tgacgcagcc tattgttgca ataacagctc caggcagccc ggcaaccttt
     7621 gttcccacaa atgtagctgc attaattgct atgggtcccg gtgtcatttg tgatatggtc
     7681 agcaaatcag caaactccgc tacagtaagc cagtttttca catccaaaac ctgaccttct
     7741 attaagggaa gagcggcata accaccgccc aggctgaata atccaatata aaagaaactt
     7801 acgaatattt ctaaatatat cattgacttt cacctgcctt tctaacgaag ttatagtaga
     7861 atcctccgaa agccccgctg ataattatta tcaaggcagc atttatgtca aaaatcacgg
     7921 ttgcaattaa agcaataacc ataattgcta tagaaacatt gtttttgctt tttataatat
     7981 cctttgtcat ttttattatt acatctatta ttacggcagc cacaccggca cgcattccca
     8041 aaagaagtgc gcttacaata gagttttctt taaaagcttc atagaatttt gctattattg
     8101 ttataattat caggggtggc aatatcgttc caaatgttgt tattaaagct cctggaattc
     8161 cagccagctt gtaccctacc ataatggatg tattcactgc aataggcccg ggcgctgact
     8221 gggatatggc tactatgttc agcatttctt cttcatctat ccatttaagc tcttccacaa
     8281 atttctttct catgagaggt acaattacat agccccctcc gattgtaaaa gcgctgattg
     8341 taaatgttga taaaaataat tgcctgtaaa aattaatgtt tcttcccata tatgtcctca
     8401 ctttcataca agtaaattta ctgattgaaa aaatagggtt agggcatcta catcaatggg
     8461 gcgaatattc tgagcaagag cctttttatc tgtttatact tagggatgtt tgttatatct
     8521 tccatctcaa cctggctgct tacccttaat gcctccaatg tgctttcttt tacctgccaa
     8581 acgctagccg agttatacaa ccagactccg cattcaaaat gcaggtaaag gcttctgaaa
     8641 tccatattta ttgttcctac tacggcatat tcatcatcca caacaaatgt cttgccgtgg
     8701 ttaaagccgg gtgtatactg gtaaatggat acccctgctt ccataagttc cccgtaatta
     8761 gactgggtca cagcaaatgc ataccatttg tccggtatga agggagtaag tatccttaca
     8821 tcgactcctc ttttagctgc cattgtcaat gccactatca ttttattgtc tataattaga
     8881 tatggtgttt ctatgtaaat atatttttct gcctggttga ttaagttcaa atacacggtt
     8941 tcgctgacat gttcactgtc taaggggctg tcatgaaagg gctgcacata cccgtggcat
     9001 tcctggcttt ggtcgtaatc atccggtctg aatttatcat attcttcatt gttttttctc
     9061 agataacccc acatggacaa aaacattaca gtaaaagacc atacggcatc accctttatt
     9121 tcaatgcagg agtctctcca gtgtccgaat tttttgacaa cattaatata ttcatctgcc
     9181 aggttcacac ctcccgtata agccaccttc ccgtctatta cgactatttt cctgtggttt
     9241 ctgttgttca tgcgaaatga caaaaccggt atatatggat taaaaacact gcatttaatt
     9301 ccatagcctt caagtatttt ataataattg tgaggaagtc ttctgacaca gccgaagtca
     9361 tcataaacta ttctcacatc caagccttgt tctgcttttt ctttcagaat acctaaaatt
     9421 gtattccaca ttaatccttc ttcaattata aaaaattcca taaatatata cttttctgcc
     9481 ttttttaatt ctctggtgag atgctcaaaa aactcctccc cggaaggcag aaattctgtt
     9541 gctgaattat cgtaaacagg acaggatgaa tatcgctcta tgtatatagc ctgattaaat
     9601 gcgattttat tttccttgtt tagattataa agggtaacag gatgttgatg aaggcattcc
     9661 ttcattttat ctacaatcgt atgcatattg ttttccattc gatttgataa ccctgttccg
     9721 ccgaacatca aataaaacaa tccgccaaac acaggaaaaa gcataattgg aataatccat
     9781 gcaattttat atgcggggtc gcttttgcgg tttaatatgt acaataccgc cagcacgctg
     9841 ataaccgtaa aaactatgtc aaatagtaaa aaataactgc tgaatctata taccatcaat
     9901 gccagggtta ttatttgagc taaaagtgcc agtgatacca aggtgaatct gcttaacaaa
     9961 aagctgatga atttttccat taaaccacct tttctcttta taatacgtaa attgtatcaa
    10021 atattgaagt gtttgtaaat gaaattgtta tttaccggca ggttcgacag gattcgtgga
    10081 ttaaaaaaag gctgccgaag cagcctgatt tgttagggca tcttgcagtc tcg
//
LOCUS       DAOPUT010000053         9812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_6966_length_9812_cov_18.014964, whole genome shotgun sequence.
ACCESSION   DAOPUT010000053 DAOPUT010000000
VERSION     DAOPUT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6966_length_9812_cov_18.014964"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1298)
                     /locus_tag="PKK75_05725"
     CDS             complement(174..1298)
                     /locus_tag="PKK75_05725"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ83132.1"
                     /translation="MKRDKYFEEYFLKKRNDISFISLKKGTAVSFKGKTYITKIELPV
                     PVRIEKLLEDINKQDEIDGITLNNIIDGIIYILGTDNNFKYKQDYKEMFEALNFEFLP
                     YIIYIINNGIKTEDSVIYGKALINNEENEKTCFIYASCLEKMGMELHEKGNDQSRYFL
                     EEANLYFEKCLDYNDKFSLAYYKLGYYYKRKQQYVKAQLIWQRHQELDDDDLRIEEIR
                     NELLQLKPYVDYENGYNLVLKERPEEGLDLLLPLVKDFSGWWNLLFFIGLAYRALGEY
                     AIAEAYFENVLKIDNLQKDALNELGLCKICRGKYVEAAELFSKLLSIDPGKCEVFCNR
                     AAAYLYNNQLDRAKEDIQTALKISPNDPVALSIKEELDRI"
     gene            complement(1285..2997)
                     /locus_tag="PKK75_05730"
     CDS             complement(1285..2997)
                     /locus_tag="PKK75_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HNZ83133.1"
                     /translation="MKILLAALDSKFIHANLALRYLKKYCSGFDIEIKEFTINQKQEY
                     ILGEILDADADLVCFSCYIWNIDYINDISYVLKEAKNKAGILYGGPEVSFEVKKMMED
                     KPFIDYIIFGEGEETFKEFLEEIQNPDPKLHKIKGLAFKENNEIIINQGRELIENLDI
                     ISYPYEEDDDFENKIIYYESSRGCPFCCSFCMSSIDKKMRFFSMERVKKDLKMLLNTK
                     AKQIKFVDRTFNADYRRSMEIIQYIVENNKSNMTIHFEITADIINDEFVNFIGKMPVN
                     MFQFEMGVQSLNEDTLFEINRHMDKGRFTRVVSEIGKNRNVHMHLDLIAGLPYEDFES
                     FKKSFDGIHSLNAEKIQLGFLKVLKGTKIYKDIEKHRIKYTKKPPYEVISTKYMSLED
                     LLMLKNIEELVDKYYNEKYFSNSLKYIINNLFPESAFEFYSSFSVYWKGNDLYNKAHS
                     RKDLYKILYDYIKYSNRMKDEFIQALKFDYIFNNQYEELPDYLNRESEDKFKNIKRLL
                     ANDREFKNNYFSSMEGKIINDFRIAEIGENNILFVYRDKENIFNRCETYVVNNIGEIG
                     NEKR"
     gene            complement(2994..3848)
                     /locus_tag="PKK75_05735"
     CDS             complement(2994..3848)
                     /locus_tag="PKK75_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83134.1"
                     /translation="MQDRINNLLSFCYNDTNSIEFQLFLITVKCLIPYEESIGCRFDK
                     DRYKKEIELFEHYMNGSDKTIDLLLKNKNPSDIEDELLEYKIIPIVLSNTIWENVIHE
                     VLSAVLFYTINKRSILNALLISSAVHEFMKNNDVESIETLSRERIIDFSVKEILAKNG
                     IRVEKEYLIEFEKERIKMIARPMLFGGNGINFYKVLNYIFDKETERPGGNKAGITNSF
                     SMYLDKLRKGLISPEKLMIPDKIPDIKECLKYPAFNHPLLGRCKVVKRTENEAIVRNK
                     SGLMRIRI"
     assembly_gap    3878..3977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4257..4955)
                     /locus_tag="PKK75_05740"
     CDS             complement(4257..4955)
                     /locus_tag="PKK75_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNZ83135.1"
                     /translation="MKREYYLTILIILCIFSYFMMLKTSITAVAEDKQNYYNLPQEPI
                     IKLFIYDYRDSFIREKQLTSFVQNLTGLEYNCCRFLIEESKGKYIDPFIVLGVIKRES
                     DFNPNAMGSGGELGLGQLMENTAEIIAGNLGYSYRHEMLLDPEFNLKLTITQLSYLKR
                     VYGKDMHKALTAYNRGQQGLENYMKAEDERGVSAYSLKVLQFASDFKEEFEKSSKLYE
                     ELMIVKEEKVIILY"
     gene            complement(5154..6860)
                     /gene="argS"
                     /locus_tag="PKK75_05745"
     CDS             complement(5154..6860)
                     /gene="argS"
                     /locus_tag="PKK75_05745"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNZ83136.1"
                     /translation="MDFKEKVAEIFTSIETEMSIDDALQLIEIPPSADMGDYAVPCFK
                     FAKKYRKSPAAIANEMVEKIGTAEEFEKIESVGPYVNFFVNKSQFAQKVLKRAFEEKE
                     NFGSTNVGEGKTVIVEFSSPNIAKPFHIGHIRSTLIGYALYNIYTYMGYNTVAINHLG
                     DYGTQFGMLISAYKKWGTPEVIEAIEKDPINELMKLYVRYNQEIETHPEYQEEARYWF
                     KQLETGNEEAHKLWSWFREVSLKEFNRVYDMFGIKYDSFAGESFYSDKMPAIIDELED
                     KCLLKDSEGARIVELSQYGLTDALIQKSDGSTLYVTRDVAAAKYRKDTYDFYKNIYVV
                     GAQQKLHFDQWRKIIDLMGYDWAYDCVHVMFGTVSLEDGSLSTRKGRVVYLEDVLNKA
                     IEKTTEIINERNPNLEDREKVAKQVGIGAVIFQELFNNRIKDYVFSWDKTLSFEGETG
                     PYVQYTYARTCSLLRKADVEIDDDVDFSVFTDSDSFNAIKKLEGFKEAVLNAHDKYEP
                     FMVTRYIVGLAQEFNRFYHECPIITDDERVKKARLLLTLTVNRVLKKGMELLGMEAPE
                     KM"
     gene            complement(7074..8153)
                     /locus_tag="PKK75_05750"
     CDS             complement(7074..8153)
                     /locus_tag="PKK75_05750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ83137.1"
                     /translation="MEREIVRLDMARDFAAFEELSVISFGQATNSKKEMYEWLFDKNP
                     YNKSGNMMYLLKEGDKVIGCDGLLPNELYVNGKTLLTAHSVKSMTHPDYKRQGIFRTM
                     TENSCERGKKDGVDVVIGLANDQSYPAYQRFGWPTLFEKEVYVKPILINNILKRRIKI
                     GFLAELGNSIYASFMKNKLKGQMDKEIEFEILDRVPEKIQKCWDKYKSKYNVLLVRDF
                     KYLNYRYNERPDIDYTTLLAKLDNEIIGFAILHNAVANGSKMTSAVEFFTDPFNKRLI
                     RALADGIASYCYDKGIEYAVIGTGLFGQYRDVLLSNGFMVTRKPPKNNMMIAHILSDK
                     VSLDEITGHEKWHITQGDGETELDL"
     gene            complement(8299..8517)
                     /locus_tag="PKK75_05755"
     CDS             complement(8299..8517)
                     /locus_tag="PKK75_05755"
                     /inference="COORDINATES: protein motif:HMM:NF040441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HNZ83138.1"
                     /translation="MERKISSFHCPKCGKVMEEGFIHSPKQIMWSVDGVPRLFDIYDE
                     YLVPMSFRRSIKVPALRCKECKITLFEY"
     gene            complement(8533..9642)
                     /locus_tag="PKK75_05760"
     CDS             complement(8533..9642)
                     /locus_tag="PKK75_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83139.1"
                     /translation="MNEENKCSKKAVIKYAGAFIAWVVGSGFATGQEALQFFSSYGYM
                     SYGVLLINFIGFLFLGQVLLITGYEHKNVVSFNHFKYFCGEKIGAAYSGLIPFTLILI
                     MPVLLSGAGSTLNEYYGVNRYLGSAVMAVLVLATYLIGFERLVKIISAIGPAIIIFSL
                     LVGTISIANDYGSFAEVPKYRYQLSKLQTAPHWTISAVLYLSLNFLSGSVYFTELGKS
                     ADKRNDAKYGALFGALAIILKIAIMNTAILLNAENISTLAIPVLYLARKISYILGAVF
                     SLVLILGMFSSCSAMLWSICSRFTRGGKRGNRIFAVSITIIAFVLSLFSFGELIGVFY
                     PIVGYVGLIFIGSVIYKGIKSNVINKLEAADKQVL"
BASE COUNT         2807 a         2005 c         1402 g         3498 t
ORIGIN      
        1 ctgtaaggct tttacgccct tgtcttttgt gacagctttg ttagtttatc ataactaaag
       61 attaaagtca ataattttta cgtatttttt ccccattttt tttcctggtt ttttcgctga
      121 tttatatttg ttttttggtt taagcaaaag ggaatcaata tcaaaacttc atatcatatc
      181 ctatctaatt cttccttgat gctcagcgcc acaggatcat tgggagatat tttcaatgct
      241 gtttgaatat cttcttttgc tctgtcaagc tggttgttat ataaataagc tgctgccctg
      301 ttgcagaaaa cctcacattt gcccgggtca atacttaaaa gctttgagaa cagttctgct
      361 gcttcaacat atttacccct gcatatttta cacaatccca attcatttaa tgcatccttt
      421 tgcagattgt caatttttaa cacattttcg aaatatgctt ctgcaatagc atactcgccc
      481 agagctctgt atgcaaggcc tatgaaaaac aacaggttcc accagccgct aaaatccttg
      541 accaggggca gcaataaatc cagaccttct tccggtcttt cctttaatac taaattatag
      601 ccgttttcat aatcaacata gggttttagt tggagcagtt catttcttat ttcttctatt
      661 cttaaatcat catcatccaa ctcctggtgt ctctgccaaa tgagctgagc ttttacatat
      721 tgctgcttcc ttttataata ataacctaac ttataataag caagcgaaaa cttatcatta
      781 taatcaaggc atttctcaaa atataaattg gcttcttcca ggaaataccg gctttgatca
      841 tttccttttt cgtgcagctc catccccatc ttttccagac aagaggcgta aataaagcag
      901 gttttttcat tttcctcatt attaattaag gcttttccgt aaataacact atcttctgtc
      961 ttaatgccat tattaattat ataaataata taaggaagaa actcgaaatt taacgcttca
     1021 aacatttcct tataatcctg tttatatttg aaattgttgt cggttcctaa aatatatatt
     1081 atcccgtcaa taatattgtt cagtgttatg ccgtctattt catcctgttt atttatgtct
     1141 tccaaaagtt tttcgattct aacaggtaca ggcagctcta ttttcgtaat atatgtttta
     1201 cccttgaagc ttactgccgt gccttttttt aagctgataa acgaaatatc atttcttttt
     1261 ttcaagaaat actcttcaaa atatttatct cttttcattt cctatctctc ctatgttatt
     1321 aacaacatac gtttcgcatc tgttaaaaat attttccttg tccctatata caaataatat
     1381 attattttcc ccgatttcag caattctgaa atcatttata attttccctt ccattgaaga
     1441 gaagtaatta tttttaaatt ctctgtcatt tgccaaaaga cgctttatat ttttaaactt
     1501 atcttcagac tccctgttta aataatcagg cagttcttca tattggttat tgaatatata
     1561 atcaaatttt aaagcttgaa tgaattcgtc tttcatcctg tttgaatatt taatataatc
     1621 atacaatatt ttataaagat ctttcctgct gtgagctttg ttgtataaat catttccctt
     1681 ccagtatacg ctgaatgaac tgtaaaactc aaaagcagat tcaggaaata agttattaat
     1741 tatatatttc aaggaattgc tgaaatattt ttcattataa tatttatcaa ccagttcttc
     1801 aatgtttttt agcatgagca agtcttccaa actcatatat ttcgttgata ttacttcata
     1861 tggtggtttt tttgtatatt tgatccggtg tttttctata tctttatata ttttcgtccc
     1921 cttcagaact tttaaaaatc ccagctgaat tttctcagcg ttaagactat gtattccgtc
     1981 aaatgatttt ttgaagcttt caaaatcttc atagggcagc cctgcaatca aatccagatg
     2041 catatgtaca ttcctattct ttcctatttc actaaccacg cgggtaaatc tgcctttatc
     2101 catatgtctg tttatttcaa aaagagtatc ttcatttaat gattgaaccc ccatttcaaa
     2161 ctgaaacata ttgacaggca ttttaccgat aaaattcacg aactcatcat taataatatc
     2221 agcagtaatt tcgaagtgaa ttgtcatgtt acttttgttg ttttccacaa tatactgaat
     2281 tatctccatt gaccgcctgt aatctgcatt gaatgttctg tccacaaact taatctgttt
     2341 ggcctttgta tttaaaagca tttttaagtc cttttttact ctttccattg aaaaaaatct
     2401 cattttctta tcaatggatg acatgcagaa gctgcagcaa aaagggcatc cccttgagga
     2461 ttcatagtat attattttat tttcaaagtc atcatcctct tcataggggt aactgattat
     2521 gtcaagattt tctataagct ctcttccttg gtttataatt atttcattgt tttctttgaa
     2581 agccagaccc ttaattttat gaagtttcgg gtccgggttt tgaatttctt caagaaattc
     2641 tttaaatgtt tcctcccctt ctccaaaaat tatataatca atgaatggct tgtcttccat
     2701 catctttttt acttcaaacg atacctcagg accaccgtat aatatcccgg ctttattttt
     2761 tgcttccttt aatacatatg aaatatcatt tatgtaatca atattccata tatagcatga
     2821 aaaacaaaca aggtccgcat ctgcatccaa aatttcccca agtatgtatt cctgcttttg
     2881 gttaatggta aattctttta tttcaatatc aaaaccactg cagtattttt tcaaataccg
     2941 cagggctaaa tttgcatgaa taaatttcga atccagtgct gccaacaata ttttcatatt
     3001 ctaatcctca taagcccgga tttattcctg actattgctt cgttttcagt tctctttaca
     3061 accttgcacc tgcctaaaag cggatggtta aatgcaggat attttaagca ttccttaata
     3121 tcgggaattt tatcaggtat catcaatttt tcaggactta ttaaaccttt tcttagttta
     3181 tccaaataca tggaaaagct gtttgttatg ccggccttat ttcctccggg acgttcagtt
     3241 tccttatcaa atatgtaatt taacacctta tagaagttaa tgccattgcc tccaaacagc
     3301 ataggtctgg caatcatttt tattctttct ttttcgaatt caattaaata ttctttttct
     3361 acccttattc cattttttgc aagaatctcc ttaactgaga aatcaataat cctttccctg
     3421 gacaaagttt caatgctttc aacatcattg tttttcataa attcatgaac cgcagatgaa
     3481 ataagcaagg catttaagat agatctttta ttaatggtgt aaaataatac tgcacttaaa
     3541 acctcatgga tgacattttc ccatatagta tttgacagca caatgggaat aattttatac
     3601 tctaaaagtt catcctctat atcagacgga tttttattct taagcagcag gtcaatcgtt
     3661 ttgtcgctgc cgttcatata gtgctcaaat agctcaattt cctttttata cctatcttta
     3721 tcaaacctgc atcctatact ttcctcatat gggataaggc atttgacagt aattaaaaac
     3781 agctgaaatt caatgctgtt tgtgtcatta tagcaaaaac tcagcaggtt atttattcta
     3841 tcttgcatat aatctcctca ttgcaggaaa atgcaacnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnagc aggttattta ttctatcttg catataatct cctcattgca
     4021 ggaaaatgca acaaatttat ataattgggg acattcctcc gtcccgcaaa gaccttctcc
     4081 atagtagagg tgtgtcccca taccttggat tcgcacaaat ttggcattct ttgcatgtga
     4141 cctgccattc attcctgctt cgctatcgct tgaagcaatt gtgttagttc acaaaaagaa
     4201 ttcgcagtct ggctgcgatt ccattataca ttcaattata taaattatca aatgctttaa
     4261 taaagaataa taactttctc ctctttaaca atcataagct cttcatacag cttagacgat
     4321 ttttcgaact cttccttaaa atcagatgca aattgtaaaa ccttcaatga ataggcgctt
     4381 acccctcttt catcttcagc tttcatatag ttttcaagcc cttgctgacc tctgttgtat
     4441 gcagttaatg ccttatgcat gtccttgccg taaaccctct taagatatga aagttgtgtt
     4501 atggtaagtt ttaaattaaa ttccgggtct aaaagcattt catgtctata tgaatatccc
     4561 aagtttcctg caataatttc tgctgtgttc tccataagct ggccaagccc cagctctcct
     4621 ccggaaccca tggcatttgg attaaaatca ctttctcttt ttattacacc cagtacaata
     4681 aacgggtcta tgtattttcc cttgctttct tcaataagaa accggcagca gttgtattcg
     4741 agaccagtca gattttgcac gaacgaggtc aattgttttt cccggataaa tgaatctctg
     4801 tagtcatata taaataattt tattattggt tcctggggta aattgtaata attttgttta
     4861 tcctctgcaa ctgctgtaat gctggttttt aacatcataa agtaagaaaa tatgcatagt
     4921 attattaaaa tagtcagata atattcccgt ttcatttcac acctccttgt cctataatat
     4981 attatacggc aagttaaatt tgtactcaat gtagaagctt gaaatgattt gttggaaatt
     5041 gatagaattt gacacttatg taatattttt ttatgaatcg cccccagctg cccggttata
     5101 aagacatgta aaaaaggaac gctcagcgtt ccttatatta atagttgttg ttgctacatt
     5161 ttttcaggcg cttccattcc cagaagttcc atgccttttt taagcaccct gttaactgtg
     5221 agggtaagaa gcagtcttgc ttttttaact ctttcatcat ctgtaataat tgggcattca
     5281 tgatagaacc tgttaaattc ctgggcaaga ccgacgatat atctggttac cataaatggc
     5341 tcgtatttgt catgagcatt taaaacagct tccttaaaac cttcaagttt tttaattgca
     5401 ttaaatgaat cgctgtctgt gaaaactgaa aaatcaacgt cgtcatctat ttccacatct
     5461 gcctttcgaa gaagactgca ggttcttgca tatgtgtatt gcacataagg tcctgtttca
     5521 ccttcaaagc ttaatgtttt atcccaggaa aatacatagt cttttatcct gttgttgaaa
     5581 agttcctgga atattaccgc acctattcca acctgctttg caaccttctc tctgtcttcc
     5641 aggtttggat ttctttcgtt tataatttca gtagtttttt caattgcttt gtttaaaaca
     5701 tcctctaaat atacaactct tccttttctt gtcgataaag aaccatcttc cagactgacg
     5761 gttccaaaca ttacatgcac gcagtcatat gcccagtcat atcccattaa gtcaattatt
     5821 tttctccact ggtcgaaatg caatttttgc tgtgcaccga ccacatatat attcttgtaa
     5881 aaatcatatg tatcttttct atattttgca gccgccacat cccttgttac atataaagtg
     5941 gacccgtcgc tcttttgaat aagagcatca gtaagaccgt actgggaaag ttctacaatt
     6001 cttgctccct cagaatcctt aagcaggcat ttgtcttcca attcatcaat tattgccggc
     6061 attttgtctg aataaaagct ttctcccgca aaggaatcat atttaattcc gaacatgtca
     6121 tatacccggt tgaattcctt taaacttact tccctgaacc atgaccataa tttgtgtgct
     6181 tcttcattgc ctgtttccaa ttgcttgaac cagtatctgg cttcttcctg gtattccgga
     6241 tgagtttcaa tttcctggtt gtatcgtaca tacagcttca tcaactcatt tattggatct
     6301 ttttcgatgg cttctataac ttcaggtgtt ccccattttt tataagcaga aataagcatt
     6361 ccgaactgtg ttccgtaatc gcccaaatgg tttattgcta cagtattgta tcccatatat
     6421 gtataaatgt tgtataaggc ataacctatc aatgttgaac gtatgtgacc gatatggaat
     6481 ggttttgcaa tatttggaga tgaaaactca actattacgg tctttccttc tcccacattt
     6541 gtgcttccga agttctcttt ttcttcaaat gctcttttca aaaccttctg ggcaaattga
     6601 gatttattaa caaagaaatt tacatagggt cccacactct caattttttc aaactcctct
     6661 gctgtaccaa ttttttcaac catttcattt gctattgctg caggagactt cctgtatttc
     6721 tttgcaaatt taaaacaagg taccgcataa tctcccatat cagcactggg tggtatctct
     6781 attaattgaa gagcatcatc aatcgacatc tctgtttcta ttgatgtgaa aatctcagct
     6841 actttctctt taaaatccat ttctttctcc tccacgattc taatcgcata acatcttaat
     6901 acgcccatat tatcaacgga ttggcgcatt tacaatcatt ttgtagtcac aataataaca
     6961 cagcaatttt aaagtgtcaa cttaaaacag atgcatctgt tgggctgtct taataagtgt
     7021 tacttttcag aatgacaccg gctttggttt atattatttc atgagacggc agcttacagg
     7081 tcaagctctg tttcaccgtc cccctgagtt atatgccatt tttcatgacc tgtaatctca
     7141 tcaaggctca ctttatcgga tagaatatgg gcaatcatca tattgttctt tggaggcttt
     7201 cttgttacca tgaatccgtt gctcaaaaga acatccctgt attggccgaa tagtcctgtt
     7261 cctataacag catattcaat acccttgtca tagcaatagc tcgcaattcc gtctgctaat
     7321 gcccttatga gtcttttatt aaaggggtct gtgaaaaact caacagcaga ggtcattttg
     7381 gagccgtttg caactgcatt atgaagtatt gcaaagccga ttatttcatt gtcaagtttt
     7441 gcaagaagag ttgtataatc aatatccggc ctttcattgt atctgtagtt taagtattta
     7501 aaatctctta ccaaaagaac attatacttg gatttgtatt tatcccagca tttttgaatt
     7561 ttttcaggta ccctgtcaag tatttcaaat tcaatctctt tgtccatctg ccccttaagt
     7621 ttgtttttca taaatgaagc ataaatagaa tttccaagct cggctaaaaa gcctattttt
     7681 attcttcttt taagaatatt gttaataagt atgggcttta cataaacctc tttttcaaaa
     7741 agtgtaggcc agccgaatct ttgataggca ggataggatt ggtcgtttgc caggcctatt
     7801 accacatcaa ccccgtcctt ttttcctctc tcacaggaat tttccgtcat agttctgaaa
     7861 ataccctgcc ttttatagtc cggatgagtc attgatttta ccgaatgggc agttaaaaga
     7921 gtttttccat taacgtaaag ttcgttaggc agcagcccgt cacagcctat taccttatcc
     7981 ccctccttta ataaatacat catattccct gatttattgt aagggttttt atcaaacagc
     8041 cattcataca tttccttctt agagttggtt gcctggccga aagaaattac cgacaattcc
     8101 tcaaatgccg caaaatctct tgccatatcc aaccttacta tttctctttc cataaaccct
     8161 ccaaatatgt taccggtttt ctattttatg taatttccat tttattataa tatttaatgc
     8221 atgtaaatga aaatttatat atcatatgca ctatataaag gcaaagaaat ccccctttat
     8281 tctcattaag aaaagaaatt aatactcgaa gagtgtaatt ttgcattcct tgcatctcag
     8341 ggcaggtact ttgattgatc ttctaaagga cattggaact aaatattcat catatatgtc
     8401 aaataatctg ggtacgccgt ctacggacca cattatctgc ttgggagaat gaataaatcc
     8461 ctcttccatc acttttccgc actttggaca atgaaaactg cttatttttc tttccataat
     8521 attctcctca ctttataaga cttgtttatc agctgcttct aatttattaa tcacattgct
     8581 ttttattccc ttatatatta cagagccaat aaatatcaaa cccacatatc ctacaatggg
     8641 gtaaaaaaca cctataagct cgccgaatga gaataaactc aacacaaatg caataattgt
     8701 gatagaaact gcaaatatcc tgtttcctct tttccctcct cgtgtaaatc ggctgcaaat
     8761 agaccagagc atagctgagc aggatgaaaa catgcccaga atcagcacaa gagagaaaac
     8821 agctcctaat atataagaaa tctttcttgc aagatataat accggtattg ccagtgtgga
     8881 gatgttttct gcattcaaca aaattgcagt attcatgatt gcaattttca aaattattgc
     8941 aagagctcca aacagtgcac cgtattttgc atcattcctc ttatctgccg attttcccag
     9001 ttccgtaaaa tacacacttc cactgagaaa attaagcgat aaatataaaa ctgcgcttat
     9061 ggtccaatgg ggagctgtct gcagcttgct gagctggtac cggtattttg gtacttctgc
     9121 aaaactgccg taatcatttg ctatgcttat ggttcccaca agcaatgaaa aaatgatgat
     9181 agccggccct atggcagaga taatcttaac taagcgttca aatcctatca aataggtggc
     9241 tagaaccaat acagccatta ctgcagagcc caaataccgg ttaacaccat agtactcatt
     9301 tagagtagat cctgcgccgg acaataaaac gggcataatc aaaatcagtg tgaagggaat
     9361 cagccctgaa taggctgcac caatcttttc accgcaaaag tatttgaaat gattgaatga
     9421 tactacattt ttatgttcat agcctgtaat caataaaacc tggcccagga acaaaaatcc
     9481 tataaaatta atcaaaagaa ctccatagct catgtatccg tagcttgaaa aaaactgcaa
     9541 tgcttcctgg cctgttgcaa atccggagcc gacaacccag gctatgaatg ctcccgcata
     9601 tttaattact gcctttttgc tgcatttgtt ttcttcattc ataacttgat ccccttacgt
     9661 gattttcccc tgcttcttcc ctgtttgcca taaatttttc acaaataatt tctgtaacgc
     9721 tttcgaccct tttatcttat tgcaaatcct tcagtctttc aaaaagttca ttgaaggatt
     9781 ttttatttct cttgttattt taattttacg at
//
LOCUS       DAOPUT010000054         9812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_6967_length_9812_cov_16.288716, whole genome shotgun sequence.
ACCESSION   DAOPUT010000054 DAOPUT010000000
VERSION     DAOPUT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6967_length_9812_cov_16.288716"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..1257
                     /locus_tag="PKK75_05765"
     CDS             400..1257
                     /locus_tag="PKK75_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNZ83140.1"
                     /translation="MDKKIVNIDLTVPYKKIVETIGNKPIAYETTGIPFWDDEYISKS
                     MLGLHLNPDVESASRAHEFMDRSVEWIVSLLGKLDGKILDLGCGPGLYAKRFADKGFS
                     ITGIDFSQRSIAYARENYGGEKNEFIYQDYLTIDYDKEYDLAILIYCDFGVLSPENRM
                     ALLKKIYRGLKQGGLFVVDVFTSNQYKEFTDSLTVEYENGGFWRADSYINIKRNASYP
                     NKNYLEQYTIITADDCVTYNIWNHGFEPQEFKKALLNAGFASVILYGDVTGANLTDES
                     PIVCAVCRK"
     gene            1582..1920
                     /locus_tag="PKK75_05770"
     CDS             1582..1920
                     /locus_tag="PKK75_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNZ83141.1"
                     /translation="MGNKKKQFIYVLKLIPALLNEKNWTVKEEAIVKRHFAKLQGLLE
                     DGKLVLAGKTDGLDEKTFGIVIFEADSEEEAKLIMENDPAIAEGIMKAELFPYRVALI
                     QDSVSKYLKY"
     gene            2022..2402
                     /locus_tag="PKK75_05775"
                     /pseudo
     CDS             2022..2402
                     /locus_tag="PKK75_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377724.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
     gene            2853..3578
                     /locus_tag="PKK75_05780"
     CDS             2853..3578
                     /locus_tag="PKK75_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003204770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ83142.1"
                     /translation="MLTINNFSKTYKGGKKAVDNISLQIESGDIFGFIGPNGAGKSTT
                     IKSIVGVLDYEEGEILISGHSIKEEPLICKKIIAYIPDNPDLYEHLTGIQHLNFIADI
                     FGIPAAIREEKIKEYADAFEITGNLGDLVSSYSHGMKQKLAIISALIHEPKLLVLDEP
                     FVGLDPKASVILKKIMHELCYSGCAIFFSTHVLDVAEKLCNKVAMINHGKLAFAGTME
                     DMVREKEGGSLEEVYMDVVNHEE"
     gene            3605..5260
                     /locus_tag="PKK75_05785"
     CDS             3605..5260
                     /locus_tag="PKK75_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83143.1"
                     /translation="MNNIDIKAEQKNLCWLLVKNRLIAKLGINVFRYEKDTHKRNIKI
                     VLTAVIIICLIIFAGYCGAMAYGYAYLGLTELIPGIAMVISSIVTLFFTMFKANGELF
                     GFKDYDMVMSLPIPVRTIINSRFLNMYLWNTFATLLVMLPMGIVYASFTKPPLGFYIM
                     WIIGMFLTCLIPTTVAAIFGAVITAISSKFRYAGTVSTILSIAFVIVIMVFPMIATST
                     DTGLGKMFDSETGNIDATAFSAMAPVISDSLNRIYPPVKLFTKAVVNGNIGSFVTFAG
                     ISIGWYAAFVFLLSVKYRQINTAITSHISRADYKLVTLHQGSMLSALYRKTIMRILKS
                     TICVTNLLIGCVLAVLLSAAIVVAGPEKVMQGLEMSDYMQIVKGAACYVVAAMVCMTN
                     TAAVSLALEGKNIWLIKSLPISPKILYDSYLLTNLTFTVPTSIVCSILFSISLKMGFI
                     GTALMILTPLTFSLFTAAAGIVIGNRMAYYDWQEETQLIKQSMMSLIGILGGMVLITI
                     CGVIANTGIFPIQTNMITFILDILFLALTAVIYINESKRPIKE"
     gene            complement(5567..6919)
                     /locus_tag="PKK75_05790"
     CDS             complement(5567..6919)
                     /locus_tag="PKK75_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ83144.1"
                     /translation="MEHTNPLGEEKVSKLLYKFSIPAIVGMMVNALYNIVDRIYIGNS
                     PDLGANGIGGITISFPLTIILLSIGILFGQGGATLFSMNLGAKKHEGAQEALGNAFIM
                     LILVGFSYMLLSQLFLVRLLKLFGASKTILPYAVEYMRYILFGSVFQVVSMGLNNFMR
                     ADGNPKLAMYTMFLGAGTNIVLDPVFIYGFKMGMAGAAIATVISQLFSFIWVVSYFTG
                     KKSKVKLILKYIKPKFNVVFKILSLGLPGFSLQIANSLLNLILNKNLQLYGGDIAVSG
                     MGVINSVQTMLIMPIIGLNQGVQPIISFNFGAKNYDRVKNAAKLAVKTATIVTTIGFV
                     ITRLFPLQIISLFNREPDMLEFGSHAIITWFLCLPVVGFQIIGANFFQALGRYKTSMF
                     LTLTRQIIILIPSIIIFSKIWGIKGLVYAAPFADSLSFLLTASFFYYGIKHLENFKLI
                     "
     gene            7278..7514
                     /locus_tag="PKK75_05795"
     CDS             7278..7514
                     /locus_tag="PKK75_05795"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNZ83145.1"
                     /translation="MDNMEIIKHFYEYVTSNNLIDELYEYIEENCIVRIGERIIPVGI
                     EGMKQHMIEVRKTYPDLKMKIIRQYTDGDTVISH"
     gene            complement(7748..8947)
                     /locus_tag="PKK75_05800"
     CDS             complement(7748..8947)
                     /locus_tag="PKK75_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNZ83146.1"
                     /translation="MVQDIIKNILIERTVGSENNSTVLLYLEKQLEAMGYDIQKLPFL
                     CTTWETKNSYLKLNERIIGINASPFSEPCDVCGNLAFAKSLEELKGMECQNNILILGG
                     DLVSSPLQPKNYPFYYPDEHRNLIELLEKKQPAAIIAATGKHPLCGLEPFPLFEDGNF
                     LIPSAYISENMLTEIQNSNTQSLIHISIQSKKIKQNSYQLVAKKKAKKTIGKIVICAH
                     MDSKYNTLGALDNAVGIAVLLEAARKLNSSIFDIDIVPFNGEEYYEASGEVEYLKFID
                     DNQDKIFFVVNIDSPCHVGSEIAVSLYNLKADTKKLINCLMGERQNVVYGPEWYAGDH
                     AAFAFRGIPCIVLSSSDLFDGALNATHTMKDNIQTVDLSLIEYAADYINDFISVISQK
                     DNPIRCI"
     gene            9414..9674
                     /locus_tag="PKK75_05805"
     CDS             9414..9674
                     /locus_tag="PKK75_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNZ83147.1"
                     /translation="MGELLKLTNIGKEIEGQLNKAGIFTYNYLKDIGAKQAWLKILEI
                     DDSACINKLLALEGAIRGIKKSELPPKCKADLKDFYNQHKAK"
BASE COUNT         3243 a         1587 c         1903 g         3079 t
ORIGIN      
        1 ttgaaaaata tttccgttga tatgtttccg agaacgtcgg ttttgagaaa aacagtaaaa
       61 aaaccattga taagttcccg cgaacataag gtgcaaatga catcaatgct gtcctctcga
      121 gccatgtaga gacagtggta ttatgtcacg tgaaaagcct tgatattatt gggttttaag
      181 actacatcta aattgacttc tcgacctaaa gtgtgttttt gcgtttgagg gaacatatcg
      241 acgacatcta aatcggttac tccagcaggt tgagtggctg aaattgatgc gtggtaggtt
      301 gaattgtcgg aatagattgc ggtacaatat atatactcaa aaggggcaaa tagcaagtaa
      361 tactcttata ttattctgca gggaaggtgg ttggaatatt tggataaaaa aatagtgaac
      421 attgacctca ctgtaccgta caaaaaaata gttgaaacaa tcggaaataa accaattgca
      481 tatgagacaa ccggtattcc attctgggat gatgaatata tttcaaaatc aatgttgggg
      541 ctacatttaa accctgatgt agaaagtgcc tccagggcgc atgaattcat ggatcgctca
      601 gtagaatgga ttgtttcttt acttgggaaa ctagacggaa aaattctaga tttaggctgt
      661 ggtccgggac tgtatgccaa gcgttttgct gataaggggt ttagtatcac aggaattgat
      721 ttctcgcaac gctcgattgc ttatgcaaga gagaattatg gaggcgaaaa gaatgaattc
      781 atctatcaag actatctgac gattgactat gacaaggaat acgatttagc tatccttatc
      841 tactgtgatt ttggtgtact ttcacctgaa aatcggatgg ccttactcaa aaaaatatac
      901 cgtggcttaa aacagggcgg attatttgtt gtggatgttt tcacatcaaa tcaatataaa
      961 gaattcactg attcattgac ggttgaatat gaaaatggtg gattctggcg ggcagattct
     1021 tatataaaca ttaagcgtaa cgcatcctac ccaaataaga actacttgga gcaatataca
     1081 attattacag ctgatgattg tgtaacatat aacatttgga atcacggatt tgaaccacaa
     1141 gaatttaaaa aggctctgct gaatgcgggt ttcgcttcag ttattctata tggcgacgtt
     1201 actggggcaa atctcacgga tgaaagtcct atcgtttgcg ccgtatgcag aaaataacat
     1261 ataccgctaa agagaagcca gattgataca gaatttttat tgtacactga acggcatcct
     1321 acattgtatt ctcttagagc actttgcttc ttatcaagaa catgtaaaag cagaatattt
     1381 ttgtggacat gtttccgcga acggataatt ccataataac cactgccgac atattccctt
     1441 gtacgggaaa tctggaaata catatccgac atcaatatga tgctatcgcg ccatgtggag
     1501 acggtggtat tatgtcacgc gatgataata ggctctaaag agcttgcaaa aacagataaa
     1561 aatatgaaag gggtttcata agtgggaaat aagaaaaaac agtttattta cgtcttaaag
     1621 ctaataccag ctttattgaa tgagaaaaat tggactgtaa aggaagaagc gattgtaaaa
     1681 aggcattttg caaagctgca ggggttactt gaagatggaa aacttgtttt agcaggtaaa
     1741 acagatggat tggatgaaaa aacctttggc attgttattt tcgaggcaga ttcagaagag
     1801 gaagcgaaat taattatgga gaatgaccca gcaattgcag aaggaataat gaaggcagaa
     1861 ttatttcctt acagagttgc attaattcaa gattctgttt caaaatattt aaagtattga
     1921 atattagtgg tcaatataga tgttaggtgt ttgcgagtgg tcgatctaga tgttttatgg
     1981 acttttcgag gtcgtgcggt caggttggat gttgaacccc tgaggctata aaaaatttaa
     2041 aagtactttc aaatgtagaa ccaacgggca ttattagtgc atctactaac ttctcggaag
     2101 ggaggatgga agaaaagggt gagctggatc attacatcgg tgtggcaact tcaagtgatg
     2161 aaacagccga atttgatgta ttgaaaatag aaaccagtaa ctgggcggtt ttcgaatcca
     2221 ttgggccatt cccagaaaca cttcaaaatg tgtggggtaa aatatactcg gagtggtttc
     2281 tttcttcagg atatgaggta gttgaaggcc cccctgaaat tttgtggaac gagagtccag
     2341 acactggaaa tccacagtat cgaagcgaaa tctggatatc aataaagaaa aaagactatt
     2401 aattacatca atgtttgtga ttgagggtgc tcctaagcgc aggcgagacg ctacaaattc
     2461 caatttgtca ataaacctga aattagggag gttatgtcag gaaaagatat ataatgtact
     2521 tgaaaatatt tgaggttttc agattttcgg caacaaaatg ctgattgctg gaaaccttat
     2581 tttttatttt ccaaaagcag ccacaccaaa aggtgttgta gggttgagct tagagaactg
     2641 ttttttgcct tacgggttaa ctgcttaaga tgtgttttat actctctcgg atgataagtt
     2701 ttaagatttt ttcataaggg ataaacaatt tggcaaaagt ttaggtattt cgcttaattg
     2761 tattgaaatt tttccttgac gttatcaaaa acaaatttat aatcaggtta agggctttgg
     2821 tataagaaaa atgtaagata aggatgtgtg aaatgttaac aatcaataat tttagtaaga
     2881 cctataaagg aggcaaaaaa gccgtagata atataagcct tcagatagaa tcaggagaca
     2941 tatttggctt tattggccct aatggtgcag gaaagagcac aacaatcaag tcaattgttg
     3001 gtgttcttga ttatgaagaa ggagaaattt tgataagtgg acattcaatt aaagaagagc
     3061 ctcttatatg caaaaaaatt atcgcgtaca ttccggataa tccggattta tatgagcacc
     3121 taacaggcat ccagcatctg aattttattg cagatatttt tggaattccg gcagctatac
     3181 gggaagagaa aataaaagaa tatgcagatg cctttgaaat caccggaaat cttggggact
     3241 tggtttcatc atattctcat ggaatgaagc agaaacttgc aattatttct gcattaatcc
     3301 acgagcccaa gctgcttgta ctggatgagc cttttgtcgg tcttgatccc aaagcatctg
     3361 ttatattaaa aaaaatcatg catgaacttt gttattcagg atgtgccatt ttcttttcaa
     3421 ctcatgtgtt ggatgttgca gaaaaacttt gcaacaaagt agctatgatt aaccatggga
     3481 aacttgcttt tgcaggaacc atggaggata tggtcaggga gaaagagggc ggatctctgg
     3541 aggaagttta tatggatgtt gttaaccatg aagaataata aatcaatgag ggatgagtca
     3601 aaaaatgaac aatatagata taaaagcaga acagaagaat ctgtgctggc ttcttgtgaa
     3661 aaaccgcctt atagctaagc ttggcattaa tgtttttcgc tatgaaaagg atacacataa
     3721 aagaaatatt aagattgtat taacggcagt aatcataatc tgtttaatca tatttgccgg
     3781 atactgcggc gcaatggcct atggttacgc atatcttgga ctgacagagc tgattcccgg
     3841 tatcgccatg gtcataagca gcattgttac tttgtttttt actatgttca aggcaaatgg
     3901 cgaacttttt ggttttaagg attatgatat ggttatgtcc ctgcctatac cggtaaggac
     3961 aattattaac agccgttttt taaatatgta tttatggaat acattcgcca cgcttcttgt
     4021 tatgctcccg atggggattg tctatgccag tttcacaaaa cctcctttag gtttttatat
     4081 aatgtggatt ataggtatgt ttttgacctg cctgattcct accactgtgg cagctatatt
     4141 tggagcagtt attacagcaa tttcatccaa attcagatat gcaggaacag tgtcaacaat
     4201 tcttagcatt gcatttgtta ttgtgattat ggtgtttccc atgatagcaa catcaacgga
     4261 taccggtttg ggaaaaatgt ttgattctga aacaggcaat atagatgcga cggctttttc
     4321 agccatggcg cctgttattt ctgacagttt gaatcggatt tatccgccgg taaaactttt
     4381 tacaaaagca gtggtgaatg gaaacattgg ctcatttgtg acttttgcag gaatttccat
     4441 cggatggtat gcggcttttg tctttctgtt atccgtaaaa tacaggcaga tcaacacagc
     4501 tataacatcc catataagca gggctgatta taaactggta acattgcatc aaggcagtat
     4561 gctttccgca ctttatagga agacgattat gcgtatctta aagtccacga tatgtgtcac
     4621 aaatctgctg attggctgtg tccttgcagt attgctgtct gctgcaatag tggttgccgg
     4681 accggagaaa gtgatgcaag gtcttgaaat gtctgattat atgcaaattg ttaaaggtgc
     4741 agcttgctat gtcgttgcag cgatggtatg catgactaat actgcagctg tttcacttgc
     4801 gttagaagga aagaatatct ggctgatcaa atctttgcct atttcaccta aaatattata
     4861 tgacagctat ttgctgacca atctgacttt caccgtaccc acatcgatag tatgctctat
     4921 attgtttagc atttccttaa aaatgggctt tattggtacg gcactgatga tactcacgcc
     4981 attaaccttt tcgctattta ctgcagcagc cggcattgtc attggaaacc ggatggcata
     5041 ttatgattgg caggaggaga cacagctcat aaaacagagt atgatgagtt taatcggaat
     5101 ccttggtgga atggttctta tcactatatg cggggtaata gccaacaccg gcatctttcc
     5161 catacaaacg aatatgatta cattcatttt agatatattg tttttggcac ttacagcagt
     5221 aatttatata aatgaaagta aacggcctat aaaagaataa aaaatgaaag gattacaaaa
     5281 aaattaataa catccttttt taaggggtgt tatcataagg attttatcta aagaaaaaat
     5341 ttaattgtta aggagaagaa cataaatgat ttttaaagat tttctcggcg tgtttaatga
     5401 gcggattatc agtgaaagag atgaataata acaagtaaga gtattctggg gacggacctt
     5461 ttaattcaaa attctaaata aggaagaaaa ataaaaaagc gagaattata aaacagtcat
     5521 ctcgctgatt tttttattat gaaaccatat ttaaaaaata caattattat atcaatttaa
     5581 agttctccag gtgtttaatt ccataataaa agaaagatgc tgttaacaga aaagacaagg
     5641 aatctgcaaa aggtgccgca taaaccaaac ctttaattcc ccatatctta gaaaaaatta
     5701 ttattgatgg aataagaatt ataatttgtc tcgtaagtgt taaaaacatt gaagtcttgt
     5761 atcttcccaa agcttgaaaa aaattggctc caataatttg aaatcccacg acaggcaaac
     5821 ataaaaacca ggtaattatt gcatggctgc caaattcaag catgtccggt tctctgttga
     5881 acaaggaaat gatttgcagc gggaataacc tggtaattac aaacccaatt gttgtaacta
     5941 tagttgcagt tttaactgct aatttagctg cattttttac cctgtcataa tttttggctc
     6001 cgaagttaaa gcttatgatt ggctgaacgc cttgatttaa acctattata ggcattatta
     6061 acatggtttg gacgctgttt atgacgccca taccggatac ggcgatatca cctccataga
     6121 gctgcaagtt tttattcaat attaaattta acaagctgtt tgctatttgt aaagaaaacc
     6181 cgggtaatcc caaggataga attttaaata ctacattaaa cttcggtttt atatatttta
     6241 agattaattt aactttggat ttttttcctg taaaatatga aactacccat ataaaagaga
     6301 atagttgtga tataactgtc gcaattgctg cacctgccat tcccatttta aaaccgtaaa
     6361 taaatacggg atctaatact atgttagttc cggctcctaa aaacatggtg tacatagcaa
     6421 gtttaggatt accgtcagca cgcataaaat tatttaatcc catgcttaca acctgaaaaa
     6481 cagaaccaaa caatatatac ctcatatatt ctacagcata tggcaatata gttttgcttg
     6541 caccgaacag ttttaataaa cgcactaaaa ataactggct tagaagcata tatgaaaaac
     6601 caactaaaat aagcataata aatgcattgc ccaaagcttc ctgtgcacct tcatgttttt
     6661 ttgcgcctaa attcatagaa aacaatgtgg ctccgccttg cccaaataat atgcctattg
     6721 ataataaaat gattgttaaa gggaaactga ttgttattcc gcctattccg tttgccccta
     6781 aatccggact gttgcctatg taaatcctgt ctacaatgtt gtataatgca ttaaccatca
     6841 ttccaacaat agccggaatg gagaatttat ataataattt agaaaccttt tcttcgccaa
     6901 gtggattagt gtgctccata tgtttactcc gtcatataaa attgatattt aattttacta
     6961 caaggttctt tatataacaa tagaaataat aaaatgttgt gtatttaaaa aagaactatt
     7021 ttaatctttt tccgtcaaat cagggaaagt ccatctaaaa accttgaaat ataagtttat
     7081 atgtcaaagc ttatatgcta caatgataat aactaaaaaa taatgaagac catagctgca
     7141 aattaaccat aaataaacaa taatctcaat aaataacgga ggccccggcg caattggaat
     7201 tgcagggatt gagtttaaca ggctgcatta atgggaaatt attctgatgt ggaaaaggtg
     7261 aaaggagtag tataaatatg gacaatatgg aaataattaa acacttctat gaatatgtca
     7321 cttcaaacaa tttgattgat gaactttatg agtacattga agaaaactgt attgtaagaa
     7381 taggcgaaag aattattccg gtaggtattg agggtatgaa acaacatatg attgaagttc
     7441 gtaaaactta tccggattta aaaatgaaaa ttattcgcca atacactgat ggtgatacag
     7501 taatctcgca ttgatcacca catcagtcct gcttgaggga gagtttggtg acaatccgtt
     7561 gtattccgtt ttcggattcg tagtggcaat tgtgttgtta ctaataggat tattaagaat
     7621 acgaatattt cacaagtcaa aaccataggg aaatttagat gggagagatt gttcatgtgc
     7681 gcaaagcaat cgcagtaatt agcgggttga ctatttctgt atgggtatat ttttataaca
     7741 acccatacta aatgcagcgg attggattat ctttttgtga gattaccgaa atgaagtcat
     7801 tgatatagtc agctgcatat tcaattaggc ttaggtcaac agtttgaatg ttatctttca
     7861 ttgtatgagt agcatttaat gctccatcaa acaaatcaga tgagcttaaa acaatacagg
     7921 ggattcctct gaatgcaaat gcggcatggt cgcctgcata ccattctgga ccatagacaa
     7981 cattttgcct ttctcccatc aagcagttaa taagcttttt tgtatcagct tttaaattat
     8041 aaagagatac agctatttca gaaccaacat gacaaggaga gtcaatattt acaacaaaga
     8101 aaattttgtc ttgattgtca tcaataaact tcagatattc aacttctccg ctggcttcat
     8161 agtattcctc accattaaac ggtacaatat ctatatcaaa aatggaacta ttcaattttc
     8221 ttgctgcttc caataaaact gctataccta ctgcattgtc aagtgctcca agtgtgttat
     8281 acttagaatc catatgcgca catatcacga tttttccaat ggtttttttt gctttcttct
     8341 ttgcgacaag ctgataacta ttttgcttta tcttttttga ttgtatcgat atatgtatta
     8401 aggattgggt attactattt tggatttcgg ttaacatatt ttcacttatg tatgcagatg
     8461 gaatcaaaaa attaccatct tcaaaaagtg gaaatggttc caagccacat agcgggtgtt
     8521 ttcctgttgc tgcaataatt gctgcgggtt gttttttctc aagcaattca ataagatttc
     8581 tatgttcatc cggatagtaa aaaggatagt tcttaggctg tagaggactg gataccaggt
     8641 caccgcctaa aatcaaaata ttgttttgac attccatgcc ttttaattct tctaaagatt
     8701 ttgcgaatgc taaatttcca catacgtcac aaggctctga aaaagggctt gcatttattc
     8761 cgataatgcg ttcatttagt ttcagatagg aatttttcgt ttcccaagta gtgcataaaa
     8821 aaggcagctt ttgtatgtcg tatcccatag cttccagttg tttttccaaa tataaaagaa
     8881 ctgtgctgtt gttttccgaa cctacagtgc gttctattaa tatgtttttt attatatctt
     8941 gtaccattaa aattcctcct ctctgtaatt tctcctcttt gggtatcatc caagcccggc
     9001 caaatttcat ggtgccatca atgcgatttg ctgcacacaa tttttgtatt tttcgttctg
     9061 aaatgcccgt tttctgaagc cgctttcaca gaaatgcaat ctatacaatc agcttcctat
     9121 cattaaaaca atatacactc attatatacg atattgcgga aaacagcaaa aataaatatt
     9181 tcatgcatgt tttcaccttc atttttatgg gtaaaagtat tatatagaaa tgaaggaagc
     9241 aaacaattga aaacttaaag aatattgtat tggacaacaa gattattgcc gattttattc
     9301 ctttgcttat tgtataataa caaaacaata tttatctatg ccacaataat tcattgaggg
     9361 gattcatttg tcaaagacga aaatttggat tatactaaac aggagttttt attatggggg
     9421 aattattaaa attaacgaat atcggaaaag aaattgaagg gcagttaaat aaagcaggca
     9481 tatttaccta taattattta aaagatattg gagcaaaaca agcatggcta aaaatactgg
     9541 aaattgatga ttctgcgtgt attaataaat tgttagcact ggaaggtgca attcgcggga
     9601 tcaagaaatc agagctacca ccgaagtgta aagctgacct taaagatttt tataatcagc
     9661 ataaagctaa atagaaaatc tttctgtgca aggaatgtgg aaaacctgaa agataagaat
     9721 aaactattat aaaattcgat atggaggggg attattgttt aatgaaagac ttatacgaga
     9781 aatttgctta tgactacgac gaatttggcc aa
//
LOCUS       DAOPUT010000055         9351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_7399_length_9351_cov_20.615856, whole genome shotgun sequence.
ACCESSION   DAOPUT010000055 DAOPUT010000000
VERSION     DAOPUT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7399_length_9351_cov_20.615856"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /gene="deoD"
                     /locus_tag="PKK75_05810"
     CDS             complement(<1..680)
                     /gene="deoD"
                     /locus_tag="PKK75_05810"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNZ83148.1"
                     /translation="MSTPHNNAVSGQIAETVLLPGDPLRAKFIAENFLDDVEQFNAVR
                     NMFGFTGTYKGKKVSVMGSGMGCPSIGIYSYELIHMYGVKNLIRIGSCGAYDPGLELY
                     DIILAMGASTDSNYAYQYDLPGTYSATASWELLSKAKKAADENNIETFVGNIVSSDIF
                     YHDNPENWKKWTKMGCLAVEMETYALYCNANRAGVNALTILTVSDSLVHQTETTPEQR
                     EKGFKDMM"
     gene            complement(726..1622)
                     /locus_tag="PKK75_05815"
     CDS             complement(726..1622)
                     /locus_tag="PKK75_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNZ83149.1"
                     /translation="MIKLGEMQELEVAKKVDIGVYLKSEDNEKPEDRVLLPAKQVPAD
                     AKIGDRIKVFIYKDSNDRVIATVKRPRIVMGEIAALKVVEMSRIGAFLNWGLEKDLFL
                     PFKEQIGDIKLNGEYMVGLYIDKSNRLCATMNLFKVLRTDSPYKVNDLVKGTVFSLKR
                     GLGAMVAVDGKYLGLIHEGELIKPLHSGQTVEVRVSNIKEDGKLDLSLKDAPKLQIDK
                     DGEKIIKTLIRNKGSLSLNDDSSPEEINRVLGMSKSSFKKAAGHLMKKKVIIMTKSGI
                     KIPEDDAKQQPVRRADIRNRKK"
     gene            complement(1676..2974)
                     /locus_tag="PKK75_05820"
     CDS             complement(1676..2974)
                     /locus_tag="PKK75_05820"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNZ83150.1"
                     /translation="MDNIKLDLHGSFIEENELKTIKPEIMAAHEMLLDRKGVSSEMTG
                     WLDYMYRMDKNEYREIKEAANYIKKNSQAFILLGIGGSYLGAKAAIEAVRGDFHNELL
                     SPQVYYAGQNLSGQYLKKLIQVIKEKEVCIIVVSKSGTTLETALAFRIFRDLMEEKYG
                     EKSNDRIFVVTDRDKGALKSMSELKGYKTFVVPDDIGGRYSVITPVGLLPMAAAGLDI
                     DKFIEGLKSGAKEYSEKNFESNISLLYAAARNILNRRNKEIEIFVNYEPSCISIAEWW
                     KQLYGESEGKEGKGIFPASLSFTTDLHSMGQFVQEGREILFETTLSIENSEDDMEVPF
                     DSNDFDKLNYISGKTLNYINSKAFEGTFMAHNEGNVPGIIIRIPKMDEFYLAKVFYFF
                     MMSCGISGYTIKVDPFTQPGVEAYKKNMFRLLRKGDTPSI"
     gene            complement(3094..4179)
                     /locus_tag="PKK75_05825"
     CDS             complement(3094..4179)
                     /locus_tag="PKK75_05825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNZ83151.1"
                     /translation="MEEYNNLDTDQISIVDGMIDWVRVVDKDNIVLYANKKMQDDLGE
                     KMVGKKCYDVLCRDKKCDNCISSTTLENGSIMRKQHSFDNKTYTIISSPLYGKDGEIV
                     GSVEVFRDITKEVLLSEKISEKNKKMSDDIKFAKNMQKRMLPPKGTYNGISIDYLYES
                     SESLSGDYFDVFEIDENNTGIYICDVVGHGVTASLLTIFVRQSLRTIAKGNMSLNRIM
                     KELHKTFLSLNLDHDKYFSLFFGIYNKDTHEFKYVNAGHNSVPILISDKTIKKIRITG
                     YPIANIFDSVDYNTGKVALNVGSKILLYTDGITEATNSRGEQFGIDNLMNIIKSGGNI
                     LEDIESAINNFSTIHKDDYAVLLAEII"
     gene            complement(4163..5158)
                     /gene="yvcK"
                     /locus_tag="PKK75_05830"
     CDS             complement(4163..5158)
                     /gene="yvcK"
                     /locus_tag="PKK75_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HNZ83152.1"
                     /translation="MKIVAFGGGTGLSILLRGLKNYTKDISAVVTVADDGGGSGVLRE
                     DLGMLPPGDMRNCIIALSDIESTMIKLMQHRFSDGYLKGQSFGNLLIAAMYEIFGSYE
                     HALKEIGSIFRLSGKVLPMTLQDAQLKAYLENGDCILGEKYIPEYVSKSNTKIDRISL
                     VPDLCRPLEETIEDIKEADVIVIGPGSLYTSIMPNILVEGIPSLIAESKADVFYVCNL
                     MTQPGETDNYNVLDHVNAIIKHSGKHMIDYIIANDEKISEELIVNYKYKGAKQVFIDR
                     DQIKQLEGMNIKVICSNFVDIRKNYIRHNSDKIGQLLFNFVGEKNKECDTYGRIQ"
     gene            complement(5158..6015)
                     /gene="rapZ"
                     /locus_tag="PKK75_05835"
     CDS             complement(5158..6015)
                     /gene="rapZ"
                     /locus_tag="PKK75_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNZ83153.1"
                     /translation="MELVIITGMSGAGKSQAIKVLEDISYYCMDNLPPALLPNFAELC
                     KSSSMDVNKVAVVADIRGGIFFKDLFNSLEELKNKGIGYRILFLDASDDDLIKRFKEQ
                     RRPHPLSAEGTIIEGILEERSRLEEVKKKSDYIIDTTNMTLGRLKEELLNIFVKGTIS
                     YNLNITIMSFGYKYGLPQESDLVFDVRFLPNPFYIEELKDNTGNDKNVQDYVMSFKTT
                     SVFLKKLMDMLKFLLPLYIKEGKSNLVISIGCTGGRHRSVAISNYLASMLTAENYRVL
                     LNHRDAGKQ"
     gene            complement(6034..6774)
                     /locus_tag="PKK75_05840"
     CDS             complement(6034..6774)
                     /locus_tag="PKK75_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ83154.1"
                     /translation="MKLKADYHIHSTYSKNKHGKSSIEEIAQRAHELGLEEIAITDHG
                     PAHYLFGIKRNLISEAKNIVVETRKKYPNLKILYGVEANILDYGGTTDINSEIIEHCD
                     IILCGFHLAALFSSAGDFWNFIVKSQFAKNNKKLYDEMVEKNTQALVNAMNKYKIDIL
                     THPGDKIPVNIDKIARAAQETNTMLEINNSHGHLNRDELIKASKYKVQFVINSDSHIK
                     DNVGRCDNAMKAVIDSGIDLSRIINLNK"
     gene            complement(6849..7775)
                     /gene="murB"
                     /locus_tag="PKK75_05845"
     CDS             complement(6849..7775)
                     /gene="murB"
                     /locus_tag="PKK75_05845"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNZ83155.1"
                     /translation="MEYQLKINCMLDELTADFKGEVLKDEPLKAHTYFKIGGPASLFA
                     VPEDTEDLKTVLKLIKKYEIEHFVMGNGTNLLVSDDGFKGIIVKIGERFNKLQRDGNK
                     VTTGAGILLSSLAKYFANEGLAGFEFASGIPGCLGGAVPINAGAYGGEMKDVVTRVKC
                     MDMDGNIHEFSNEEMEFAYRHTKLSDSEYIVLEAEFMLNPANTEDIMEKIRDLNEKRT
                     SKQPLNLPSAGSTFKRPINGYASKLIEDAGLKGLRYRGAMVSDKHSGFIVNYDNACCE
                     DVLSLMRIVISTVYDKFGIKLEPEIKIVGTKL"
     gene            7987..9153
                     /locus_tag="PKK75_05850"
     CDS             7987..9153
                     /locus_tag="PKK75_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNZ83156.1"
                     /translation="MKNKVVELINKITPELNDLSLEIYNNPELGYEEFKACKLHTDIL
                     RKYGFDVEENFSGVKTGFKAVYKGKKDGLTIAYMSEYDALPGIGHGCGHNLLGTVSTG
                     SGIILKQIIDEIGGTVIVFGTPAEETSGAKVTYVENHEFDDVDIALMAHPASEYAKSG
                     TALALEPVQFEFFGKASHAAAAPEKGINALDAAIQTFNGVNALREHIRSDSRVHGVII
                     DGGKAANIVPEYSKVQYYVRSTTKTYNTELLEKVKNCARGAALATGTKLEITKFEFNY
                     DNMVTNQALSKVFNDTIYEVAGIVMKEPLKSTGSIDAGQVSQVCPTIHPYFDVTNDPS
                     IAGHTRDLGESTIKDYAKEQMKNTIAALVLTAAKVIQDPKLYEEIKYEFDNTEK"
BASE COUNT         2536 a         2043 c         1567 g         3205 t
ORIGIN      
        1 ttcatcatgt ctttaaagcc tttttccctt tgctccggtg tagtttcagt ctggtgcaca
       61 agagaatctg atacggtcaa tatagtaagt gcatttactc ctgctctgtt tgcattgcag
      121 tataatgcgt aggtttccat ttcaacagcc aggcagccca tctttgtcca ctttttccaa
      181 ttctccggat tgtcatgata gaaaatatcc gagcttacaa tgtttcccac aaatgtttct
      241 atattgtttt catctgcagc tttcttagcc tttgataaaa gctcccaaga ggcagttgcc
      301 gaataagttc ccggcaagtc gtactgataa gcgtaatttg agtctgtgct tgcacccatg
      361 gcaagtataa tgtcataaag ctccaaacca gggtcataag ctccgcaaga gcctatccgg
      421 atcaaatttt taactccata catatgtatc aattcgtaag agtaaattcc tattgatgga
      481 catcccatac cgcttcccat tacactaacc tttttccctt tgtaggttcc tgtaaacccg
      541 aacatatttc ttacagcgtt aaattgttca acatcatcaa gaaaattttc tgcaataaat
      601 tttgccctta aggggtctcc cggcagtagg actgtttcgg caatctgccc tgaaacagcg
      661 ttattgtgtg gtgtagacat ataaaatctc ctttttaagt attttttatt gttaattctt
      721 acttgctact ttttcctgtt tcgaatatcg gctcttctga caggctgctg ttttgcgtca
      781 tcttccggaa tcttaatgcc gctcttagtc atgataatta cctttttttt catcaaatgc
      841 cctgcagcct ttttaaaaga gcttttgctc atgcctaaaa ctctgtttat ttcttcggga
      901 ctgctgtcat cgtttaagga taagctgccc ttgtttctaa tgagcgtttt tataattttt
      961 tctccgtctt tgtctatttg cagtttcgga gcatccttga ggcttaaatc cagcttcccg
     1021 tcttccttaa tattgcttac tctgacttca accgtctgtc cggaatgaag aggctttatt
     1081 aattctccct catgtatcag gcccaaatac ttcccgtcaa cagccaccat ggctccaagg
     1141 cctctcttta agctgaatac cgttcccttt acaagatcat taaccttgta aggagaatct
     1201 gttctcaata ctttaaacaa attcattgtg gcacaaagcc tgtttgactt gtcaatataa
     1261 agaccaacca tgtattctcc gttcaatttg atatcgccta tctgttcctt aaagggcaga
     1321 aataagtctt tttcaagacc ccagttaaga aatgctccaa tcctcgacat ttcaacaacc
     1381 ttcaaggcag caatttcacc cattactatt cttggtcttt ttacagttgc tataactctg
     1441 tcattagagt ccttgtaaat aaatactttt attctatcac caatttttgc atctgcaggt
     1501 acctgctttg cgggcagcaa caccctgtcc tccggttttt cattgtcttc gctttttaaa
     1561 tagacaccta tatctacctt tttagcaact tccagttcct gcatttctcc taatttaatc
     1621 atattttctc ctcttcgggg acattccttc aaaagctatc ccgcatagta tcatcttata
     1681 ttgaaggtgt gtcccccttc cttaatagtc tgaacatatt ctttttgtaa gcttcaactc
     1741 ccggctgagt aaaggggtct accttgatag tatacccgct tatgccgcag ctcatcatga
     1801 aaaagtaaaa aaccttggca agataaaatt catccatctt tgggattcta ataattatcc
     1861 ccggtacatt tccttcattg tgagccatga atgttccttc aaatgccttt gaatttatgt
     1921 agttaagtgt ttttccggat atgtagttta atttatcaaa gtcatttgaa tcaaagggaa
     1981 cttccatatc atcttcgctg ttttcaattg acagggttgt ttcaaagagt atttctcttc
     2041 cctcttgaac aaattggccc atggagtgaa ggtcagtagt gaagctaagt gatgccggga
     2101 agataccctt tccttctttc ccttcgcttt ccccgtaaag ctgtttccac cattcggcaa
     2161 ttgatatgca ggatggctca tagttcacaa atatttcaat ttccttgttt cgcctgttta
     2221 aaatatttct tgctgctgca taaagcaggc tgatattgct ttcaaagttt ttttctgaat
     2281 attcctttgc tcctgacttt aagccttcaa tgaatttgtc gatatcaaga ccggctgcag
     2341 ccatcgggag gaggcctaca ggggttataa cggaatatct tcctcctatg tcgtcaggaa
     2401 ctacaaatgt cttgtagccc ttaagctcag acatgctctt taaagcgccc ttatccctgt
     2461 ctgttacaac gaaaatgcgg tcatttgatt tctccccgta cttttcctcc atcagatctc
     2521 tgaaaattct gaatgccagg gctgtttcca aagtagtacc cgattttgac acaacaataa
     2581 tgcaaacttc cttttccttt atcacctgaa taagtttttt caggtattgt ccgcttaaat
     2641 tttgccctgc ataatatacc tgaggactta agagctcatt gtgaaaatct ccccttaccg
     2701 cttcaattgc agcttttgcc cccagataag agcctccgat tcctaaaagt atgaaggctt
     2761 gagaattttt cttaatataa tttgcagctt ctttaatttc ccggtattcg tttttatcca
     2821 tcctgtacat gtagtcaagc cagcctgtca tttcactgct gactcctttt ctgtccaaaa
     2881 gcatctcatg agctgccatg atttccggtt tgattgtttt tagttcattt tcttcaataa
     2941 agcttccatg taaatccagt ttaatgttat ccatttttcc ctcctgactt ttatatatta
     3001 cactaccccc gtccccttgt cagatcccca cgaccagggg gacattccgt caacccgcaa
     3061 aaacttccct aaaggagcag gttttcaccc ctcctatatt atttcagcta acaggacagc
     3121 atagtcatct ttgtggattg tactgaagtt attgattgct gattctatat cttccagtat
     3181 atttccgcct gactttatga tattcataag attgtcaata ccaaactgct caccccggct
     3241 atttgttgcc tctgttatgc cgtcagtata aagtagtatt ttgctgccca catttaaagc
     3301 tactttccct gtattgtaat ccacgctgtc aaatatgttc gcaatgggat atcctgttat
     3361 tcttatcttt ttaatagtct tatcgctaat cagtatcggc actgaattat gccctgcatt
     3421 gacatacttg aactcatggg tgtctttatt atatattcca aaaaacaagg agaaatattt
     3481 atcatggtcc aaattcaaag acaaaaatgt tttatgcagt tctttcataa ttctattcag
     3541 gctcatattg cccttggcaa ttgttcttaa tgactgcctt acgaaaattg ttaaaagaga
     3601 tgccgtaaca ccatgtccca caacatcaca aatataaata cccgtattat tctcatctat
     3661 ttcaaataca tcaaaataat ccccgcttaa tgattctgat gattcataca aataatcgat
     3721 gctgatgccg ttataggttc cctttggagg aagcatcctt ttttgcatgt tttttgcaaa
     3781 ctttatatca tcactcattt ttttattttt ttcacttatc ttttctgaaa gcaaaacctc
     3841 cttggtaatg tccctgaata cttccacaga ccctacaatt tcaccatcct tgccgtatag
     3901 gggagatgaa ataatagtat atgttttatt gtcaaaacta tgctgtttgc gcattataga
     3961 cccgttctcc agggttgtgc ttgaaataca gttatcacac tttttatccc tgcataatac
     4021 atcatagcat tttttcccta ccattttttc tcccaaatca tcctgcattt ttttgtttgc
     4081 atacaaaact atattgtcct tgtcaactac cctgacccag tcaatcattc catccacaat
     4141 gcttatttgg tctgtgtcca gattattgta ttcttccata ggtgtcacac tccttattct
     4201 tttcaccaac aaaattaaac agcaattgcc cgattttatc cgaattatgc cttatatagt
     4261 tttttcttat atcaacaaaa ttactgcaga taacctttat attcattccc tcaagctgtt
     4321 ttatctggtc ccggtcgatg aaaacctgct ttgcaccctt atatttgtaa tttacaataa
     4381 gctcttccga tatcttttca tcattggcaa taatataatc tatcatatgt ttgccgctat
     4441 gctttataat tgcattaaca tggtctaaaa cattgtagtt atcagtttct ccgggttgag
     4501 tcataaggtt gcagacataa aacacatctg ccttgctttc agctataaga gaaggtatgc
     4561 cttctactaa aatattaggc attatgctgg tatacaagct tcccggcccg ataacaataa
     4621 catccgcttc ctttatatct tcaattgttt cctcaagtgg cctgcacaaa tcaggcacca
     4681 gtgatatcct gtctatcttg gtatttgact tgctaacata ttcgggaatg tatttttccc
     4741 ccagtatgca gtccccgttt tcgaggtatg cttttaattg agcatcctga agtgtcatgg
     4801 gtagaacctt ccccgaaagc ctgaatatgc ttcctatctc cttgagtgca tgctcgtagc
     4861 tgccgaatat ttcatacatt gctgcaatga gcagatttcc gaagctttgt cccttcaaat
     4921 aaccatcgct gaaacggtgc tgcataagtt ttatcattgt agactcaata tcggaaagtg
     4981 caataatgca gtttctcata tcccccgggg gcagcattcc caagtcttct cttaaaactc
     5041 cggagcctcc gccgtcatct gcaactgtta caactgcaga tatatctttg gtataatttt
     5101 taagtcctct cagcaatatg gaaaggcctg tccctcctcc aaaggcaact attttcatta
     5161 ttgtttcccc gcatctctgt gatttaataa gactctataa ttttcagctg taagcatgga
     5221 tgccaaataa tttgatattg caacagaccg gtgcctgcct ccagtacagc cgattgatat
     5281 tacaaggttt gattttcctt cctttatata caagggaagg agaaatttaa gcatatccat
     5341 aagtttcttc aaaaatacgg atgtggtttt aaaactcatt acataatcct ggacattttt
     5401 atcgttgcct gtattgtctt ttaattcctc tatataaaaa ggattgggca ggaatcttac
     5461 gtcaaacaca aggtctgatt cctgaggaag accatattta tagccgaatg acattatggt
     5521 tatgtttaaa ttataagata tggtgccctt cacaaaaata tttaaaagct cttccttaag
     5581 ccttccaagt gtcatatttg tagtgtctat tatataatcg gatttctttt ttacttcttc
     5641 caatctgctt ctttcttcca gtattccttc tatgatggtc ccttcagcag acaggggatg
     5701 aggtcttctt tgttccttga acctttttat caaatcatca tcagaggcat caagaaacag
     5761 tattcgatat ccgattcctt tatttttcaa ttcctccaaa ctgttgaaca aatccttgaa
     5821 aaaaatcccc cctcttatat ccgctaccac tgccaccttg tttacatcca ttgacgaact
     5881 tttgcatagc tctgcgaaat taggaaggag ggcaggaggc agattgtcca tgcaataata
     5941 gcttatatct tcaagaacct tgattgcctg gcttttaccg gcccctgaca tgcctgtaat
     6001 tattactaac tccattttcc ctgctccttc tctttattta ttaagattaa ttatcctgct
     6061 taaatcaatg ccggagtcga ttacagcttt cattgcattg tcacaccgtc ccacattgtc
     6121 cttaatgtgg gagtcactgt ttattacaaa ttgtaccttg tattttgatg cttttataag
     6181 ctcatccctg tttaagtggc cgtggctgtt gtttatttca agcatggtat tcgtctcttg
     6241 tgctgcccgg gctatcttgt caatattaac cggtattttg tcccccggat gagtcaatat
     6301 atcaatttta tatttattca tggcatttac aagagcctga gtgttttttt caaccatttc
     6361 atcgtaaagc ttcttattgt ttttggcaaa ttggcttttt actatgaaat tccagaagtc
     6421 acctgccgat gaaaataaag ctgccaggtg aaagccgcat agaattatat cgcaatgctc
     6481 aataatttca ctgttgatat cagtagttcc gccataatcc agaatattgg cttctacacc
     6541 gtacaaaatt ttcagattgg ggtatttttt tcttgtttcc accacaatat tcttggcttc
     6601 acttatcaag tttcttttaa tcccgaacaa atagtgggca ggcccgtggt cagtgattgc
     6661 tatttcctcc agtcccaatt catgggccct ttgagctatt tcttcaattg atgacttccc
     6721 gtgtttattt tttgaatatg tcgaatgtat gtgataatca gcttttaatt tcatctgggt
     6781 ttccttgttt tattttcata acattttata gtgtctgaac gtcccgaaat ctcagggtta
     6841 gggcatcttt aaagcttagt tcctactatt tttatctccg gctctaattt aattccaaat
     6901 ttatcataaa ccgtgctgat tacaatccgc atgagggaca aaacatcttc acagcaggca
     6961 ttgtcataat taactatgaa accgctgtgt ttgtctgaaa ccatggctcc cctgtatcta
     7021 agccccttca gtcctgcatc ttctattaat ttggaggcat agccgtttat aggcctcttg
     7081 aatgtgcttc cggcgctggg taaatttaat ggttgcttgg aggttctttt ttcattaagg
     7141 tcccttattt tttccataat atcttcagtg ttagcgggat ttagcataaa ctctgcttca
     7201 agaacaatgt attcagaatc cgacagcttg gtgtgtctgt aggcaaattc catttcttca
     7261 ttggaaaatt catgaatgtt tccatccata tccatacact tgaccctggt taccacgtcc
     7321 ttcatttcac cgccgtaggc tcctgcattt atgggaactg ctcctccaag gcagcccggt
     7381 attccgcttg caaattcaaa gcctgccagg ccttcattcg caaaatattt tgccaaagag
     7441 gataaaagta ttcctgctcc tgtcgtcact ttgtttccgt cacgctgaag cttattgaac
     7501 ctttccccta ttttcactat tattccttta aatccatcat ctgaaaccag gagatttgtt
     7561 ccgtttccca taacaaaatg ttctatttca tatttcttta tcagctttaa aactgttttt
     7621 aagtcttctg tatcttcagg cactgcaaac agacttgcag gacctccgat tttaaaatat
     7681 gtgtgagctt tcaaaggctc atctttaagt acttccccct tgaagtccgc tgtcagttca
     7741 tcaagcatac agttgatttt taattgatat tccatgattc catcctttcc tgtcaactaa
     7801 ctacatttta acattgaatt gatatttttt aaagatatat ttttagtata tgctgtttaa
     7861 aacttgtttt acaaattagt ttcaaaaaat tagattggtc cttgaattgg tttgacatag
     7921 attattaaca atgctagtat atttatgtac ataacaacaa tatttattaa tattttggga
     7981 gggaatatga aaaacaaagt agtggaatta atcaataaaa ttacacctga gcttaacgac
     8041 ttaagccttg aaatctataa taatccggag cttggatatg aggaattcaa agcatgcaag
     8101 cttcatacag atattttaag aaagtacggc tttgatgtgg aggagaactt ctcaggtgta
     8161 aaaacaggtt ttaaagctgt ttataagggc aaaaaggatg gtcttacaat agcatatatg
     8221 tccgaatatg atgctttgcc gggaatagga cacggctgcg ggcataattt attgggaaca
     8281 gtcagtacag gttcaggtat tatattaaaa caaataattg atgaaattgg aggaactgta
     8341 atagtattcg gcactcctgc cgaagaaacc agcggtgcca aggttacata tgttgaaaat
     8401 catgaatttg atgatgttga tatcgcttta atggcacacc cggcaagcga atacgcaaaa
     8461 agcggaacag cccttgcact ggagcctgtt caatttgaat ttttcggcaa ggcatcccat
     8521 gccgctgctg ctccggaaaa gggtattaat gctttggatg ctgcaattca aacatttaac
     8581 ggtgtaaacg ccttgagaga gcatatcaga agcgattcca gggtacacgg cgttattatt
     8641 gacgggggta aagctgcaaa tattgttcct gaatattcaa aggttcaata ctatgtccgc
     8701 tcaacaacca aaacatacaa cacagagctt ttggaaaagg tcaaaaactg tgcccgtgga
     8761 gctgctttgg caacaggaac aaaattggag ataacaaaat ttgagtttaa ctatgataac
     8821 atggtaacaa accaagcctt gtcaaaagtg tttaacgata cgatttatga ggttgccggg
     8881 atagtgatga aggaaccttt aaagagcacc ggttccatcg atgcaggcca ggtaagccag
     8941 gtatgtccta caattcatcc ctatttcgat gttacaaatg acccttcaat agccggacac
     9001 acaagagatc tgggagaaag caccataaag gattatgcta aggaacagat gaaaaataca
     9061 atagcagccc tggtattgac agctgcaaaa gtaattcaag acccaaaatt gtatgaagaa
     9121 ataaaatatg aatttgataa cacagagaag taaaaaaggg actgacgtcc cttttttgtt
     9181 tcaatcgaat tcttatttgc gcatggtccc tgtttttatg tatctttcat gccaggacag
     9241 ggcttcttaa aacgggccgt agttgatcat tactgctatt atcatgaata tgcagcccat
     9301 tactgtccat aagatcatta aaggagctac aaatttaacc cattcttcat a
//
LOCUS       DAOPUT010000056         9301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_7451_length_9301_cov_15.726693, whole genome shotgun sequence.
ACCESSION   DAOPUT010000056 DAOPUT010000000
VERSION     DAOPUT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7451_length_9301_cov_15.726693"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1158
                     /locus_tag="PKK75_05855"
     CDS             247..1158
                     /locus_tag="PKK75_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNZ83157.1"
                     /translation="MIFLLLAILCSASINVCFKISENRPDGNSKAVSTLNQATAAIVS
                     FIFMGGGKLFSLKWLTDMTYEWTNVFSSSGNRFSDSGSLGYALLVAVLGGLFSYFGLI
                     ILQNCTRKNGTVMTIAFNKLGLLVPIIFSMIFFHEKATGLQIVGMLLAVFAIMLIYFK
                     KEAADVITSPLLLIGTLFCGGFYDFVSKIYEARGSEYGQTVFVFWIFLISTAIALIAF
                     LKSGNRNINKGDIFFGCLTGVIIQLISKFLLKAISVLPSVIVFTIFSVGVIVTVNIVN
                     LLVFKEKLSKRQYIATSLMLAACLLLS"
     gene            1349..2371
                     /gene="ltaE"
                     /locus_tag="PKK75_05860"
     CDS             1349..2371
                     /gene="ltaE"
                     /locus_tag="PKK75_05860"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNZ83158.1"
                     /translation="MKFIDLRSDTVTMPTDEMRQAIANAEVGDDVYRDDPTVNKLEKL
                     AAEKVGKEASLFVPSGTFGNQLAILTHTRRGNEVIVGKNNHIVLHEVGASAVIAGVQL
                     RTLETVNGVMSPKDVLKAIRVDDIHEPETGLICVENAHGCGAVVPLSVLKEIKEIAGS
                     HSIPVHMDGARIFNAALSLGVDAKEIAACCDSVMFCLSKGLAAPAGSMLAGSKEFIDR
                     ARKYRKLMGGGMRQIGILAAAGIIALEKMTGRLAEDHENAKYLAEELSKLPGVYVKRD
                     RLDIDMVFFEMGEDVIKESLLIEKLYEKNIKINGVEGGEYRFVTHIGVSREDLDYVIN
                     CIKELM"
     gene            complement(2562..3167)
                     /locus_tag="PKK75_05865"
     CDS             complement(2562..3167)
                     /locus_tag="PKK75_05865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNZ83159.1"
                     /translation="MKKILIIILSIALITACTNNSVEVPENEIQEAIDETEGTDSQAE
                     ENNNETEVSGDKPDNNGENNDKKPVTAPDFELSSMDGTVIKLSETRDKNVILNFWYTE
                     CVFCVTEMPDLQKLQDAYPDNLQVLAVNVGESKEVIQTFMDENNLSLTVLLDEDMQVA
                     YDYGIRSFPTTIALNKKGEVVGGYIGMLTYEQMEQLYGFFE"
     gene            complement(3210..4076)
                     /gene="ispE"
                     /locus_tag="PKK75_05870"
     CDS             complement(3210..4076)
                     /gene="ispE"
                     /locus_tag="PKK75_05870"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNZ83160.1"
                     /translation="MKLKSFGKINLFLDVTGRLQNGYHIIKTVMQSVDIYDEISLVPI
                     DTGEIAIKCSDSSIPENEKNTCFKAASILKKAFGINTGIHISINKNIPQGAGMAGGSS
                     NAAAVLKGLNAIWKLDLSRGELCRFGAQVGADVPFCLVGGTCLAEGIGDKITELNDFQ
                     WNNILTVKPEFSISTAFVYENLDSRHYNSYVSEDILKHISSYSYEKAAGCVANTLEKA
                     VAEFYPEINNIKKLMIDNGAISSLMTGSGSAVIGFFKDGDSLNKALKITAEIYPYVRK
                     VKTVKDGVKFID"
     gene            complement(4348..5283)
                     /locus_tag="PKK75_05875"
     CDS             complement(4348..5283)
                     /locus_tag="PKK75_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNZ83161.1"
                     /translation="MLDIFKSIEDTMFKLEKIEDGAWINLVNPTNEELDFIENQFAVD
                     KDMLRAALDEEESARLEIEDEQVLILVDTPYVEKQDEHIIYETLPLGVIITDKNIITV
                     CLKNSVVLEEFKKNSIRTFCTYKRYRFLYQILYKNAQRYLLYLRQIDKMSNHVEEQLH
                     KSMRNKELIQLLDLSKSLVYINTSLKGNQIVLEKILRMNIIKKYSEDEDLLEDVIIEN
                     KQAIEMAAIYSGILSGTMDAFASIISNNLNIVMKLLTSITILMAIPTMFASFFGMNVN
                     NIPFANQPYGFWLIIGIALLTSTFAGIMLLKKNLL"
     gene            5563..5820
                     /locus_tag="PKK75_05880"
     CDS             5563..5820
                     /locus_tag="PKK75_05880"
                     /inference="COORDINATES: protein motif:HMM:NF018016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HNZ83162.1"
                     /translation="MPTSSVTRVKGAVEDCVGKKVKFKLKKGKSRSHFSEGVILDTYP
                     SIFTVFVEKNDYKRVVSFNYIDILTNNVEFYICDDKETRIG"
     gene            6018..6848
                     /locus_tag="PKK75_05885"
     CDS             6018..6848
                     /locus_tag="PKK75_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ83163.1"
                     /translation="MKLLKFTSFLILASIILYSYARFIEPVLLTVKYENIYSDLLNEK
                     NDNIKIIQFSDSHISRYFNVTNLKKAVHKINAQNPQIVVFTGDLINKFNHYDNKENIH
                     EIWEVLSEINAPLGKYAVYGNHDYGGGAERAYRQIMTNSGFKILVNENIKIKENNINI
                     IGLDDSIFGTFDKETIVSLLDENYYNIIISHEPDVAEYFLQYSTDLFLAGHSHGGQVN
                     LPFIRYTPLLAQKYIRGLYGIDNYRQTKIYVNIGLGTSTIPMRLMAAPELTVITLKNK
                     "
     gene            complement(6978..7178)
                     /locus_tag="PKK75_05890"
     CDS             complement(6978..7178)
                     /locus_tag="PKK75_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HNZ83164.1"
                     /translation="MDKFALTLIIVGALNWGLIGLFQLDLISVIFGGRIGLFTRMINI
                     LIGLAGLYSITFYFGYKFVDKN"
     assembly_gap    7327..7426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7427..9090)
                     /locus_tag="PKK75_05895"
     CDS             complement(<7427..9090)
                     /locus_tag="PKK75_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ83165.1"
                     /translation="MKKVLSLVLAALLILSSFSFAFAQDAETVKITVIHTNDTHSRLA
                     KTSSEIGFAKIAALVKEAKAANPNTLVLDAGDTLHGMPIVNISKGENAIKVLDAVGYD
                     YMTLGNHDFNHGLERLLELKDMAAIGMLSANIEDKNGEYVFTPYVIEEIGGVKVGIFG
                     LSTPDTSYLANPKYVENLTFKDPIEVSREMVAELEGKADVVIALAHLGLDESSSVTSK
                     ALAEAVEGIDLIVDGHSHTKLEAGQLVKDTLIAQTEYYGKNLGYVDIEVQNGEVVNMA
                     AKLLSVADTGETVPDPDLSAFIDSIDPKKDPAFNEVIAKTDVYLDGVRENVRTKETNF
                     GNLSADAARAATGADIAFVNGGGIREDVPAGEVTKGKIAAVFPFGNTIEVKKITGTDL
                     KAMLEWSVSDYPAAKGAFLQVSGLEFTFDPAKPVNSKVVEIKVGGEVFDEAKEYTVAI
                     NDFMSDGGDGYSMLANYDVLAISGTYEEIIQNYLATNGTAGSDVSGRIKVKETVAEEV
                     TYIVVPGDVLWKIAAKYGYTYQELAEYNNIANPHLIFPDQVIKVPQ"
BASE COUNT         2904 a         1672 c         1617 g         3008 t
ORIGIN      
        1 atatattcgg agtacgagga aacaccttct tctattggat aatccttgag gggtgaagta
       61 attcccgaaa agaacatttt ggtgcgaaaa acgtcccaaa tggtgggtag aggcaggtaa
      121 gaaaagcatc gagatgtgta aaactacagg agatgtgatt tatcaaaaaa tcttccggca
      181 aaccggaatt aagagtcaaa ttcccggatg acaactgaga cttcaaaata aagataggtg
      241 ttaataatga tttttctgtt acttgctatt ctttgctctg caagtataaa cgtatgcttc
      301 aaaatatctg aaaaccgtcc ggacggcaac agcaaagcag tttccactct taaccaggca
      361 actgccgcaa tcgtatcatt catcttcatg ggtggcggaa agcttttctc tctaaaatgg
      421 cttactgata tgacctacga atggacaaat gttttcagtt cttccggaaa ccgcttcagc
      481 gatagcggtt cccttggcta tgcccttctt gtcgcggtac ttggaggctt gttcagttat
      541 ttcggcttaa taatactcca aaactgcaca cgcaaaaacg gtactgttat gacaatagcc
      601 ttcaacaagc taggccttct ggttccaata atcttttcca tgatattctt tcatgaaaaa
      661 gccaccggcc tccagatagt cggcatgctt cttgctgttt ttgcaatcat gctcatatac
      721 ttcaaaaaag aagctgcaga tgtcataaca tctccgcttc tgttgatagg gactcttttc
      781 tgcggcggtt tttatgattt tgtctccaaa atctatgagg cacgcggcag tgaatacggc
      841 cagactgtat ttgtcttttg gattttcctt atttcaactg ctatcgcatt aattgcattt
      901 cttaaaagcg ggaacagaaa cattaacaaa ggagatatct tcttcggctg cctgacaggc
      961 gtaataattc agctgatttc caaatttctt ctgaaagcta ttagtgttct tccatctgtc
     1021 attgtcttca caattttttc cgttggtgtc attgtaacgg tcaatatagt taatcttctt
     1081 gttttcaaag aaaagctttc aaagcgccag tatattgcca ccagtctcat gttggcagca
     1141 tgtctgcttc tttcatagta ttctgaggat tgtattaata cttgcgttat tatatgaagg
     1201 agactggtag atgccaaaat taatcgttga aagaaacatc taattggtat aatataaaaa
     1261 gagaatatat tgtatcttat aagaattaca attcaaatga gtaaagcata tgtcgtatta
     1321 taaaaaataa aaatagaagg tggtaaatat gaaatttata gatttaagaa gcgacactgt
     1381 tacaatgcct accgatgaaa tgagacaggc tattgcaaat gcagaagttg gagacgatgt
     1441 ttaccgggat gaccctacgg ttaacaaatt ggagaaactt gctgccgaaa aggttggtaa
     1501 ggaagcttcc ctgttcgtac ccagcggtac ctttggaaat caacttgcaa tacttacaca
     1561 tacacgacga ggcaatgaag taattgtagg aaaaaataat catattgtac ttcatgaagt
     1621 aggagcctcg gcagttatag ccggagttca gcttagaacc ctggaaactg taaacggcgt
     1681 catgtctcca aaggatgttt taaaagccat aagggttgat gatattcatg aacctgaaac
     1741 aggcctcata tgtgtagaga atgcccatgg atgcggagca gtggttcccc tttcagtatt
     1801 aaaagaaata aaagaaatcg caggaagcca ttcaattccg gtccacatgg acggagcaag
     1861 aattttcaat gcagcattgt cattgggcgt ggatgcaaaa gaaattgccg catgctgtga
     1921 ttctgtaatg ttctgcttgt caaagggatt ggcagctcct gcaggttcaa tgcttgcagg
     1981 aagcaaggaa tttattgaca gggctagaaa atacagaaag ctaatgggtg gaggaatgag
     2041 gcagatagga atactggctg ctgcaggaat catagcactg gagaaaatga ccggcaggct
     2101 tgctgaagac catgaaaatg ctaaatattt agcagaagag ctctctaagc tgcccggtgt
     2161 ctatgtaaaa agagacaggc ttgacatcga tatggtattt tttgaaatgg gtgaggatgt
     2221 aataaaagaa agtctgctga tagaaaaact ttatgaaaaa aatattaaaa taaacggggt
     2281 tgaaggcggc gagtaccgct ttgtaacaca tataggagta tcaagggaag atttagatta
     2341 tgtgataaac tgcattaaag aacttatgta acaaatattt tgatgaagtg accgagtggg
     2401 caaaaatccc atattaaaag agggtattgc cctctttttt tgtattgatt aatataggga
     2461 tactccctct tccctggaat agccgttagg aacaaagaaa tatatccaaa tagaatattt
     2521 ttatgaaggt gacttccagg ggcactgttt ctaaagccgg actactcaaa aaacccgtaa
     2581 agctgttcca tttgctcata ggtaagcatt cctatatagc cgccgactac ttcgcctttt
     2641 ttgttcaaag caatagtagt ggggaaggaa cgaatgccgt aatcataagc cacttgcata
     2701 tcctcatcca ataaaactgt taggctaagg ttattttcat ccataaaagt ttggattact
     2761 tccttgcttt cccctacgtt gactgccaat acctgaaggt tatcaggata tgcatcctgg
     2821 agcttttgca aatcaggcat ttctgttacg cagaatacgc attccgtata ccagaaattt
     2881 aaaattacat tcttatccct ggtttcacta agctttatta cagtcccatc cattgaactt
     2941 agttcaaaat ccggtgccgt taccggcttt ttatcattgt tttcaccatt gttatcaggt
     3001 ttatcaccgg aaacctctgt ttcattgttg ttttcttcag cctggctatc agtaccttca
     3061 gtttcatcta tagcttcttg aatttcattc tcagggactt caactgagtt atttgtgcat
     3121 gcagttatca aggctataga taaaattata atcagtattt ttttcataaa accctcctat
     3181 aaattgattg atatccttta taagtttatt taatcgataa atttaacccc atctttaacc
     3241 gtcttaacct tccgtacata aggataaatt tctgctgtaa tttttaatgc tttgttaagg
     3301 gagtcaccgt ctttaaaaaa accaataacg gcagaacctg agccggtcat tagggatgat
     3361 attgcaccat tgtcaatcat tagtttctta atattgttga tttccggata gaattctgcc
     3421 acagcctttt ccagtgtatt tgcaacacat ccggcagctt tttcataact atatgatgat
     3481 atgtgcttaa gtatgtcctc cgacacatag gaattatagt gtctggaatc aaggttttca
     3541 tacacgaatg cagtagaaat tgagaattcc ggctttactg ttaatatatt attccattgg
     3601 aagtcattta gttcagtaat cttgtcacct attccttcag ccaggcaggt gcctccaacc
     3661 aggcaaaatg gaacgtcagc accaacctgt gctccaaaac ggcacaattc tccccttgac
     3721 aaatcaagct tccagattgc attcaaaccc tttagcacag cagctgcatt ggaactgcct
     3781 cctgccatac cggctccttg agggatattt ttatttatgc ttatgtggat gcctgtattt
     3841 atgccgaaag ctttcttcag tatggacgca gctttaaagc aggtgttttt ttcattttcg
     3901 ggtatggatg aatcagaaca cttaatggca atttcgcctg tatctattgg aacaagagat
     3961 atttcatcat aaatatcaac agattgcata acagtcttga ttatgtgata accattttgg
     4021 agtcttcctg taacatccaa aaataaattg atttttccaa atgattttaa tttcatagtt
     4081 caactcatct aaaagttttt catcattata taacataaac ttttttttaa caatgaaaaa
     4141 gataattaat tatgaatttt atgattaaaa tttaaaataa tgggtataaa tttaatgaat
     4201 acaagttacc aaggaagagg tgattccccc aaaagcagaa attggaacac taaacgaata
     4261 acacccggtt tctggtgtga aaatgacgaa aacgggtgtt attcattttt tgtataacat
     4321 ggacattacc ggtgtgttcc cataccccta taacaaattc ttttttaaaa gcataatccc
     4381 tgcaaatgtt gatgttagca atgcaatacc gattatcagc cagaaaccat aaggttgatt
     4441 tgcaaaggga atattgttta cattcatacc gaaaaagctt gcaaacattg tcggtattgc
     4501 cattagtata gtaattgacg tcaacagctt catgacgata tttaaattat tggatataat
     4561 agacgcaaat gcatccattg ttccgcttaa aattccgctg taaattgcag ccatctcaat
     4621 ggcctgctta ttttctataa taacatcctc caacaaatcc tcatcctcgg aatatttttt
     4681 tattatattc attctcagaa ttttttctaa aactatttgg ttgcctttaa gggaagtatt
     4741 aatatatacc agtgacttgg ataaatccaa aagctgtata agctccttgt ttctcattga
     4801 tttatgaagc tgctcctcta catgattgct cattttatct atttgcctca agtaaagcag
     4861 gtatctttgc gcatttttat atagtatctg atataaaaaa cgatatcttt tatatgtgca
     4921 gaaagtacga atactgtttt tcttaaactc ttccagtaca accgaatttt ttaaacatac
     4981 agttattatg tttttatcgg taattataac cccaagagga agtgtttcat aaataatatg
     5041 ttcatcttgt ttttcaacat atggtgtatc taccaatatt aaaacttgtt catcttctat
     5101 ttctaaacgt gcactttcct cttcgtccag ggctgctctt aacatgtctt tgtcaacagc
     5161 aaactgattt tcaataaagt ccaattcttc atttgtggga ttaaccaggt taatccatgc
     5221 accatcttca atcttttcca atttgaacat ggtgtcttca attgatttaa aaatatcaag
     5281 catttcttca cctcaattta ataatagatt ttctctatta ccaaattgag ttatatccag
     5341 taatagagaa gttataatag atttttgacc ttagggatct cgtccattag ttcacttcct
     5401 tttaattttt atgcatatca aaacaacggc atagtatgcc atacattaca attataccgc
     5461 aatttgaaga taatgtacat attttttatt tatatgggaa agatattaaa aataaatagt
     5521 cttttcccgg ttttaataaa aaaaggcata gaaggtgatt ggttgcccac aagttctgtt
     5581 acaagagtta aaggagcagt tgaggactgc gttgggaaaa aggtaaaatt caaattgaaa
     5641 aaaggaaaga gccggtccca ttttagtgaa ggagtaatac tggatacata cccttcaata
     5701 tttacagtct ttgtagaaaa aaatgattat aaaagggttg tgtcttttaa ttatatagat
     5761 attctaacaa ataatgttga attttatata tgcgatgaca aggaaacaag aataggatga
     5821 tgtaagttaa tagtgatctt ttttattaaa aagaatttaa taaataaatt tcgctatttt
     5881 tttttgcgga tatttgatat acttaagtat ctaagcccat ttctaagggt tttggggaca
     5941 taccttccac cggtgacagt tttctagggt gaaggaattt tccataaatt attactatac
     6001 tattcaggag aagattagtg aaattattaa aatttacaag ttttttaatt ttagcttcaa
     6061 ttattcttta ttcatacgca agatttattg agcctgtttt attgacggtg aaatatgaaa
     6121 atatatacag tgatttatta aatgaaaaaa atgataatat aaaaataata cagttttcag
     6181 attctcatat atcccggtat tttaatgtga caaatttgaa aaaggcagta cataaaataa
     6241 atgctcaaaa ccctcaaata gttgttttta caggtgattt gattaataaa ttcaatcatt
     6301 acgataataa agaaaatata catgaaattt gggaggtttt aagcgaaata aatgctccct
     6361 tggggaagta tgctgtttac ggcaaccatg attatggcgg cggtgcagaa agagcatata
     6421 ggcagataat gacaaacagc ggattcaaga tactagtaaa tgaaaacatt aaaatcaaag
     6481 aaaacaatat taacataata ggattggatg attcgatttt cggtaccttt gacaaagaaa
     6541 caatagtcag cttattggat gaaaactatt ataatataat tataagccat gaaccggacg
     6601 tagcagaata ttttctgcaa tacagcacag atttattttt ggccggccac agtcatggag
     6661 ggcaggtaaa tctccctttt atcaggtaca cccctcttct ggcgcaaaaa tatatcagag
     6721 gattgtacgg gattgacaat tacaggcaga ctaagatata tgtcaatatt ggcctgggaa
     6781 catcaaccat acctatgcgg ttaatggctg cccctgaatt aacagtgatt accctaaaaa
     6841 ataaataatc agagtgtaaa aaaagtcata agattaagca tgaaatcttt tgacttttat
     6901 cgtttcgaag tagaaaaaac atttctctgg taacgtattt tgggaattcg ttgcgaacga
     6961 tatatttatt ctactggtta gtttttgtca acaaatttat aaccaaaata gaatgtaatg
     7021 ctgtataagc ctgccaaacc tatcagtata tttatcattc gagtaaataa tcctattctg
     7081 ccaccaaaga taacagagat caagtctaac tgaaataatc caattagtcc ccagttcaat
     7141 gccccaacta ttattaatgt caatgcaaat ttatccaatt atcattgtcc tcccttgaat
     7201 atgtatgaaa tataaaattt ttacgattaa aggctttgct gtatcagcaa agccttttgg
     7261 agaattatta tttttgagga acttttatta cctgatcagg gaaaattaaa tgtggatttg
     7321 caatatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttt gaggaacttt
     7441 tattacctga tcagggaaaa ttaaatgtgg atttgcaata ttgttgtatt cagccaattc
     7501 ctgataagta taaccgtatt ttgctgctat tttccacaaa acgtctcccg gtacaactat
     7561 gtaggttact tcttcagcta cagtttcttt tactttgatt ctgccggaaa catcgcttcc
     7621 tgcagttcca ttggttgcaa gatagttttg aattatttct tcatatgtac ctgatattgc
     7681 aagtacatcg tagtttgcca acatagaata accgtcaccg ccatctgaca taaagtcatt
     7741 tattgcaact gtgtattctt tagcttcatc aaacacctcg ccgcctactt ttatttccac
     7801 aactttggaa tttacaggct tagcaggatc aaaagtaaat tccagtccgc ttacctgcag
     7861 gaaagcaccc ttagcagccg gataatcgct tactgaccat tcaagcatag ctttcagatc
     7921 tgtgcctgta atttttttaa cctcaattgt attaccaaag gggaatacag ctgctatctt
     7981 tccttttgtt acctcacctg caggaacatc ttctcttata ccgccaccgt taacaaatgc
     8041 aatatcagca cctgtagcag ctcttgctgc atcagcggaa aggttgccaa aatttgtttc
     8101 cttggttctc acattttctc taacaccgtc caagtaaaca tcggttttag ctattacttc
     8161 attaaaagca ggatcttttt ttggatctat tgaatcaata aatgctgata aatccggatc
     8221 cggaacagtt tctccagtat ctgcaactga aagcagcttt gctgccatgt ttacaacttc
     8281 accattttga acttctatgt caacataacc taagttttta ccataatatt ctgtttgagc
     8341 tatcaaggta tcttttacca attgaccggc ttctaatttt gtatggctgt gtccgtcaac
     8401 tattaagtcg attccttcta cagcttctgc cagagcctta gaggttacgg aggagctttc
     8461 atcaagtccc aaatgtgcca gtgcaataac aacatcagct ttgccttcaa gttcagctac
     8521 catttctctt gatacttcta taggatcttt aaatgttaag ttttcaacat atttaggatt
     8581 tgcaagatat gatgtatcag gagtagaaag gccgaaaata ccaaccttca ctccacctat
     8641 ttcttcaata acataaggag taaaaacata ttctccattt ttatcttcaa tatttgcact
     8701 cagcattcca attgcagcca tgtccttaag ttccaacaat ctttctaaac catggttaaa
     8761 atcatggttt cccagtgtca tatagtcata tcctacagca tccaatacct tgattgcatt
     8821 ttctcccttg gagatgttta caataggcat accgtggagt gtatctcctg catcaagaac
     8881 caatgtgttt ggatttgctg ccttggcttc cttaacaaga gctgctattt tagcaaagcc
     8941 aatttcagaa ctagttttag ctagtcttga atgggtgtca ttagtgtgaa taacagtaat
     9001 ttttacagtt tctgcatctt gtgcaaatgc aaagctgaaa ctgcttagaa ttagcaatgc
     9061 agctaatact aaacttaata cttttttcat taatttatcc tcctattttt tttatgtatg
     9121 acatacttat attatatagg cagagggatg gattttcaag acaacttttg atgattttat
     9181 atatttgggg atattcctct accatcagag aatatcacat gagtagaggt gcatcctcat
     9241 gctgataatt gcagaaaaac tcctgaaaat tgttgacaat aagattaaat ttttatatta
     9301 t
//
LOCUS       DAOPUT010000057         9188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_7562_length_9188_cov_17.663199, whole genome shotgun sequence.
ACCESSION   DAOPUT010000057 DAOPUT010000000
VERSION     DAOPUT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7562_length_9188_cov_17.663199"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1088)
                     /locus_tag="PKK75_05900"
     CDS             complement(96..1088)
                     /locus_tag="PKK75_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNZ83166.1"
                     /translation="MFIDEYELDSITDNPYLNEIYKEAVFIDVETTGLSRTYSDIISI
                     TFLLYEDDKYKIYQIFCQYKIDQPDALKHLKEMIKSKKYIVTYNGNSFDLPMLEEKAK
                     KYSIALSFDLFIKIDLYNYVQKLRYKINTHDLKLKTLEEYFCIERNESLGGKDIITLY
                     EAYKLEPRKEFSYLILKHNYEDVYNLPFVMNNIFNLYDDVIYLKNIIVPINNDDISIK
                     KNSLQCNFNVLTIIDKNFINHSMNYDMKLYVETNVLEIIIPLNTYKDESIGDFYYLDN
                     NEYEIDSYTAIKGIIRNLIPIKINDKMYYNNINSIVRKIIEEGFGHSKMQMQAY"
     gene            1156..3465
                     /locus_tag="PKK75_05905"
     CDS             1156..3465
                     /locus_tag="PKK75_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNZ83167.1"
                     /translation="MYIIISIFAAYALGVISYELNMFSYYFTAVFAILIYSSIINKKF
                     VYNAVIIAFVILSYLNCNYNSSSVLSQYINENIVIEARIKDKRNTGSMSCNASVISIN
                     KTPLLNEENIILYTGEYKNFKENSIIKVSGNVAGNQISKNRMLFNYENYLRSKKIKAS
                     VFAEGDALIMEENYSFLNNTSQKFKYYTERTFYNNLNKNNADIILSIILGDVSYLEEE
                     LYDNIKVMGLAHIFAVSGTHIVFMYVFLLNVFKFFFSRRVSWILSWFFIWFYGFLIGF
                     PLSVMRALVMFTLLFGSEVIYRKYNSLNSIGLAALILTVYNPFWIFDAGFLLSFSASL
                     SFIIYSNYIKTKSTVFDTIYMYLFLQIFTLPVIVYYFNFIPVMGVLYNLLLLPVFTVI
                     MIYGFILLILNSFLHFIFIVPFKIFDYILHSLRLIIDISNEFAFNGIIFPSMSICHMI
                     FFYIALFFMIYLHNNELSCKKYGIAAIIYFYSITYIAFPMMDDSFYLNIADAGQGLFT
                     SIKYKGADIIYDCGSNSTKQMGEYTALPYLTKRGISEVDAIFISHWDDDHYSGLDDIL
                     NSHIKVKNIFSSKDNENYKVQELNAGYKMKFDDNLSMEILWPYKDLAFNNINNSSLVI
                     SLNYKDIRILLTGDIEEEAEYAIYNELNSCDIVLVPHHGSNTSSTVQFVDAVRPKIAL
                     LSYGRNLYGIPHQEVIFRYENAGSKIFSTFEQGEINIVLKDNNLYYNTYINEKSDNYY
                     ELYLYGIGYNLSIFCFLLLWMLKGDSYEL"
     gene            3455..4483
                     /gene="holA"
                     /locus_tag="PKK75_05910"
     CDS             3455..4483
                     /gene="holA"
                     /locus_tag="PKK75_05910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNZ83168.1"
                     /translation="MSFDLIKSMGDKNKLPNCLLLYGDEEYFMDYSVKYIKNKYIDKS
                     YENMNYAEFEKLDSLKDFFEFADTFPFMSEKKLCLLKEAGFFTSTGSLDKKEEEKLLK
                     YIDGNEDCITIFVIKDGKPDSRKKIVKKLKDNKAVFEFTRLNEGELTKYITDEFKKNS
                     FKISMSDANYMANNTGYLDYESSVSLYHVNNEISKIMSHNNEKKNITTSDLELLMVKS
                     VESSIFRLVDCICENNKKKSFEILDEMLLNNTPEQFIIHMITRQYRMLYQYVILQKKG
                     YTFNEIINKMKIKNFVASKLEKQSKNLKMETIEYYMRRFLEIDRKIKAGEIDSRIGLE
                     IITNGIIS"
     gene            complement(4511..4774)
                     /gene="rpsT"
                     /locus_tag="PKK75_05915"
     CDS             complement(4511..4774)
                     /gene="rpsT"
                     /locus_tag="PKK75_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNZ83169.1"
                     /translation="MANIKSAKKRILIINKKTEVNKSRKSEIKTYIKKFESALSEGNY
                     EKAQEYLKICEKKLYQAAAKNAVHRNAAARKVSRMTRKLNKAA"
     gene            5130..6065
                     /gene="gpr"
                     /locus_tag="PKK75_05920"
     CDS             5130..6065
                     /gene="gpr"
                     /locus_tag="PKK75_05920"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR01441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HNZ83170.1"
                     /translation="MMYRTDLAYEANESLAKKFKGITLKTHDYSHGEVVELEIKDEEA
                     EKAVGKKMGKYITYETKSLKLLSEKSRNEVIDILSKAIRDISGLDRGKVLVVGLGNRN
                     ITADALGPRTLDKIKVTGQYFKTYKKEYDKDFNDVSILEPGVLGTTGIETINTIAGVV
                     GIIKPTLIIIIDALASRKMRRLCAVVQVTDAGIEPGSGIGNMQGSLNKESIGTKVIAL
                     GIPTVVDTATIVNDTIEMMEEALKDKTDDVGQIMGILSDLNYNEKHAFIKEILSPMYG
                     ESIVTPSYVDEIIDSLSGLLAESINRAVHPGYVNS"
     gene            6186..7304
                     /locus_tag="PKK75_05925"
     CDS             6186..7304
                     /locus_tag="PKK75_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HNZ83171.1"
                     /translation="MKRRRKNKSGRSFYMVMSILCLTALFLGYSFVYNLSEERKTGKD
                     ITTISIKYNASGKEDDLSTFDRVMSYFNIFLEKTFKEKKSDDVDIIENIEPQSHKNEF
                     FKTIASQEPERDLEIFKEDSYEPEADGEIYLADDKKESFFKVIQTTSRSSSSRRKLFL
                     KTAPNVDGLNVVLFHTHGTEAFQSAYDSKYRSLDESLNILGIGTKISNSLLNQGINNI
                     HLKGYNDYPDYNKSYANSNYAVRQVLSDSKKNVLIDLHRDGAEENSNYEAILSEVKTI
                     EINGKNAATCTLVTGNKNKNAGALKENAGRLYDIAYEMYPGLFRKIIIREGAYFNQYL
                     SDYSLLIEVGCTLNTYEEIEYTAELLTEVLYEYINEIQ"
     gene            7379..>9188
                     /gene="lepA"
                     /locus_tag="PKK75_05930"
     CDS             7379..>9188
                     /gene="lepA"
                     /locus_tag="PKK75_05930"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNZ83172.1"
                     /translation="MFLDRQKYIRNFSIIAHIDHGKSTLADRLIENTGLLTHREMQDQ
                     VLDNMDLERERGITIKLQTMSLLYKAQDGHSYTLNLIDTPGHVDFSYEVSRSLAACEG
                     AVLVVDAAQGIEAQTLANVYLALDQDLEIVPVINKIDLISARPDEIKQEIEDVIGLDC
                     SDAPLISAKENINIDQVLEAIVKKIPPPEGDEDAPLKALVFDSYYDSYRGVVAYIRIK
                     EGQVKKGDKIKMMSTGKTFDVTETGIISPFQKPVSCLYSGDVGYLCASMKEVKSCQVG
                     DTITNSANPAKDPLPGYKKVTSMVFCGIYPAEGEEYNSVRDALEKLQVNDASLTYEPE
                     TSVALGFGFRCGFLGLLHMEIIQERLEREFDLNIIATTPSVIYKVYKTDGQMMILQNP
                     TNMPPVQEIEYMEEPIVAASIMSPTEYVGAIMELCQKRRGTFVNMEYMEATRVILHYK
                     LPLNEVIYDFFDALKSKTRGYASLDYELDSYVQSELVKMDILINSELVDAFSIIVHEE
                     KAYERGRAIVERLREVIPRHLFAVPLQAAIGNKIIARETVKALRKDVLAKCYGGDITR
                     KKKLLEKQKEGKKRMRQIGMVEVPQEAFLAVLKFDED"
BASE COUNT         3188 a         1374 c         1694 g         2932 t
ORIGIN      
        1 gagcaggaga ctgaaaatct ccgtgtccgt ggttcgattc cgcgtttggg caccaaaaaa
       61 gacttataca atctgtatat gtctttattt tttattcaat atgcctgcat ctgcatttta
      121 gaatgaccaa atccttcttc tattattttt cttacgatac tgtttatatt gttataatac
      181 atcttatcat ttatttttat cggtatcagg tttcttatta ttccttttat agcagtatag
      241 ctgtcaattt cgtattcatt gttatctaaa taatagaagt ctcctatact ttcatcctta
      301 tatgtattta aaggaataat aatttccaaa acattagttt cgacgtatag tttcatatca
      361 taattcatgc tgtgatttat aaaattcttg tctataattg tcaaaacatt gaaattacat
      421 tgaagggaat tcttttttat tgaaatatca tcattattaa ttggaacaat aatatttttt
      481 aaatatatga catcgtcata caaattaaaa atattgttca taacaaaggg taaattataa
      541 acatcctcat aattgtgctt aagtatcaaa tatgaaaatt cctttcgagg ttctaatttg
      601 tatgcttcat acaatgtgat aatatccttg ccccccaggc tttcatttct ttcaatgcag
      661 aaatattcct caagagtttt tagttttaaa tcatgggtat taattttata tcttaatttc
      721 tgtacataat tatataaatc tattttaatg aataaatcga aactaagagc tatactgtat
      781 ttttttgctt tttcctccag cataggaagg tcaaagctgt tgccgttgta agttacaata
      841 tatttctttg atttaatcat ttcctttaaa tgtttaagag catccggctg atctatttta
      901 tattgacaaa atatttgata tattttatat ttgtcatctt catagagcaa aaaagtaatg
      961 gaaataatat ctgaataagt ccttgacaag cctgtagttt caacatctat aaatactgct
     1021 tccttataaa tttcatttaa gtaaggattg tctgtaatac tatctagttc atactcatcg
     1081 ataaacattg ttccccctga ataattgctt tgaatttcca cccaatattt ggtaaaatat
     1141 taatgggtgt tgtgaatgta tataataatt tcaatatttg cagcttatgc actgggcgta
     1201 atttcatatg agttaaatat gttcagctat tatttcacag ctgtttttgc catactgatt
     1261 tacagttcaa taataaacaa aaaatttgtt tacaatgctg taattattgc atttgtaatt
     1321 ttatcttatc ttaattgtaa ttataattca agctcggtcc tttctcaata cataaatgaa
     1381 aatattgtga ttgaagcaag gataaaagac aagagaaata ctggcagtat gagctgcaat
     1441 gcctcagtta tcagcataaa caaaactccc cttttaaatg aagaaaatat aatcctctat
     1501 accggtgaat ataaaaattt taaggaaaat agcataataa aagtaagtgg taatgttgcc
     1561 ggcaatcaaa taagcaaaaa taggatgctt tttaattacg aaaactattt aagaagcaag
     1621 aaaatcaaag catctgtctt tgctgagggc gatgctttaa ttatggaaga aaactattcg
     1681 tttttaaaca atacttcaca gaaattcaaa tattatactg aaagaacatt ttacaataat
     1741 ttaaataaaa ataatgcgga tataatattg tcaataattt taggcgatgt aagttatctg
     1801 gaggaagagc tttatgataa tataaaggta atggggcttg cccatatttt cgctgtttcc
     1861 ggcacacaca tcgtttttat gtatgtcttt cttttaaatg tgtttaaatt ctttttcagc
     1921 agaagagtca gctggatatt atcctggttc tttatatggt tttacgggtt tttaatcggt
     1981 tttcccttgt cggttatgag agctctggtt atgtttactc ttcttttcgg gtctgaggtt
     2041 atatacagaa agtacaattc actgaattct atcggtcttg cagcacttat tttgactgtc
     2101 tacaatccct tttggatttt tgatgcagga tttctcttat ccttttcagc atcactgagc
     2161 tttataattt acagcaatta tataaaaaca aaatccactg tttttgatac aatatatatg
     2221 tatttgtttc tgcaaatatt tactctgcct gtaatagtat attacttcaa ttttattccg
     2281 gttatgggcg ttctttataa tttactgctc ttgcccgtat tcactgttat tatgatttac
     2341 ggattcattc ttttgatatt aaattcattt ctacatttta tttttattgt gccttttaaa
     2401 atatttgatt atattttgca cagccttagg cttatcattg atatttcaaa tgaatttgct
     2461 tttaacggaa ttatttttcc atccatgtcc atctgccata tgatattttt ctacatagct
     2521 ttgtttttca tgatatactt gcataacaat gaattaagtt gcaaaaaata cggcattgct
     2581 gcaataattt acttttattc cattacctat attgcatttc caatgatgga tgacagtttt
     2641 tatttaaaca tagcagatgc gggacaaggt ttgtttacct caatcaagta taaaggagca
     2701 gatataattt acgactgcgg cagcaattca acaaaacaaa tgggggagta tacagccctt
     2761 ccttatttga caaagagggg gatttccgaa gtagacgcaa tatttataag ccattgggac
     2821 gatgaccatt attccggttt agacgatatt ttaaacagcc atataaaggt taaaaatata
     2881 ttttcatcaa aggacaatga aaactataag gtacaagaat taaatgcagg ttacaaaatg
     2941 aagtttgatg ataatttaag tatggaaata ttgtggcctt ataaagattt agcatttaac
     3001 aatattaata attcatccct ggtaatttct ttgaattata aagatataag aattttgctg
     3061 accggagaca ttgaggagga agcagaatat gcaatttata atgagctgaa ttcctgtgac
     3121 atagtattgg ttcctcatca cggcagcaac acatcctcaa ctgtccagtt tgttgatgct
     3181 gtaaggccaa aaattgccct tttaagctat ggaagaaacc tttacggcat tcctcaccaa
     3241 gaagttattt ttaggtatga aaatgcaggc agcaagattt tttccacatt tgagcaggga
     3301 gaaataaata ttgttttaaa agataacaat ttgtattata atacttatat aaatgaaaaa
     3361 tccgataatt attatgagct ctatttatat ggaatagggt acaatcttag catattttgc
     3421 tttttattat tatggatgtt gaagggtgat agttatgagc tttgatttaa ttaaaagcat
     3481 gggggataaa aacaagcttc ctaattgcct gctgctatac ggcgatgaag agtatttcat
     3541 ggattattcc gtaaaataca taaaaaataa atatatagat aaaagctatg agaacatgaa
     3601 ttatgccgaa ttcgaaaagc ttgacagcct aaaggatttt tttgaatttg cagacacatt
     3661 tccttttatg tccgaaaaaa aactatgcct attaaaggaa gccgggtttt ttacatcaac
     3721 aggaagcctg gataaaaaag aagaagaaaa gcttttgaaa tatatagatg ggaatgaaga
     3781 ctgcatcaca atttttgtaa ttaaagacgg aaagccggat tcaaggaaga aaattgtcaa
     3841 gaaacttaag gataataagg cagtttttga atttaccagg ctgaatgaag gtgagcttac
     3901 aaagtatata acagatgaat tcaagaaaaa ctcctttaaa atatccatgt ccgatgcaaa
     3961 ttatatggca aacaacacag gttatctgga ttatgaaagc agcgtaagtt tgtaccatgt
     4021 aaataatgaa atcagtaaga taatgtccca caataatgag aaaaaaaata taaccacatc
     4081 cgatttagaa cttttgatgg ttaaatctgt tgaaagcagc atattcagat tagttgactg
     4141 catatgtgaa aataataaga agaaatcatt tgaaatattg gatgaaatgc ttttaaacaa
     4201 tacacctgag caatttataa ttcatatgat taccaggcag tacagaatgc tttatcagta
     4261 tgtaatttta caaaaaaaag ggtatacctt caatgaaata atcaacaaaa tgaaaattaa
     4321 gaattttgta gcttcaaagc ttgaaaaaca gtccaaaaat cttaagatgg aaaccattga
     4381 atattatatg aggaggtttt tggagattga cagaaaaata aaggctggag aaatagattc
     4441 aaggattggg cttgaaatca taacaaacgg aataatctca taaaaaaaac cggtaatgcc
     4501 ggttattttt ttatgctgct ttattaagtt ttcttgtcat tctgcttact tttcttgctg
     4561 cagcgttcct atgaacagca ttttttgcag cagcctggta taatttcttt tcgcatattt
     4621 ttaagtattc ttgagctttt tcgtaattgc cttcacttaa tgctgattca aatttcttaa
     4681 tatatgtttt aatttcagat tttctgcttt tattcacttc agttttttta tttataatta
     4741 agattctttt ctttgcagat tttatgtttg ccaaaatatc cacctccttt atttgtatct
     4801 atattagaat ttcgtgtctt attcaccgat attatatttt gcagtctcat taaccaatat
     4861 atcatatctc gcagtctcat tcaccaatgt atttgctccc tttggccgca tacattggga
     4921 attcgttgcg aatgacctgc catcaatttt ttagaaaaaa ctctgaaaaa ttgggttagg
     4981 gcatcttaga caacaaatca attatacaga ctctcaatac aaaaatcaag taaaaagaat
     5041 aaaaaaatca aaaaaaacta taataactta aacactttga attattaaca atcaggtgcc
     5101 ggatgatgaa ttatattacg ggaggaatta tgatgtatag aacagacttg gcctatgaag
     5161 caaatgaatc tcttgcgaag aagtttaaag gcataacttt aaaaacccat gattacagcc
     5221 acggtgaagt tgtggaacta gaaataaaag atgaagaagc tgaaaaagca gtaggcaaga
     5281 aaatggggaa atatattacc tatgaaacaa aaagcctaaa gcttctttcg gagaaatcaa
     5341 gaaatgaagt tattgatatc ctgtcaaagg ccataagaga tatatcagga cttgacaggg
     5401 gtaaggtact ggtggtgggc ttgggaaaca gaaatattac agctgatgcc ctgggaccca
     5461 ggacccttga taaaattaaa gtaacaggac agtatttcaa gacttataaa aaagaatacg
     5521 acaaggactt caatgatgta tcaatattgg agccgggtgt attaggtaca acaggaattg
     5581 aaaccattaa tacaattgcg ggggttgttg gaataatcaa gcctacatta ataataataa
     5641 tagatgccct ggcttcccgc aaaatgagaa ggctttgcgc ggttgtccag gtaactgacg
     5701 caggtataga gcctggttca ggcattggaa atatgcaggg atcccttaat aaggaatcca
     5761 ttggaacaaa ggttattgca ctgggtatac caacggtggt tgatactgcc actattgtca
     5821 atgatacaat tgaaatgatg gaagaggcct taaaagataa aactgacgac gtaggtcaaa
     5881 ttatgggtat attaagcgat ttgaattaca atgaaaagca tgcgttcata aaggaaattt
     5941 taagccccat gtatggagaa tctattgtaa caccaagcta tgtggatgaa ataattgatt
     6001 ccttgtcggg cttattggca gagtcaataa acagggcagt acatcccgga tatgtaaatt
     6061 cgtaatataa ttgtaaaaat taacagtgtt attcaggaaa caatacacat atacattaat
     6121 atattacttt ttagatattt tatggagtag tatattacaa cgagaaaata tgtggagttg
     6181 agatcatgaa gagacgaaga aaaaacaaga gcggcaggtc attttacatg gtgatgtcta
     6241 ttttgtgttt gacagcactt tttttggggt acagttttgt ttataattta tctgaggaaa
     6301 gaaagacagg taaggatatt acaacaattt caattaaata taatgcaagc ggcaaagaag
     6361 atgatttaag cacatttgac agggttatgt cttacttcaa catctttctg gaaaagacct
     6421 ttaaggaaaa aaaaagtgat gatgtggata taatcgagaa cattgaacct caatctcata
     6481 aaaatgagtt ttttaaaaca atagcaagcc aggagcccga gagggatttg gaaattttca
     6541 aggaagattc ctatgaacct gaagctgatg gtgaaatcta cctggcagat gataaaaaag
     6601 agagtttttt taaagttatt cagacaacat caagaagctc ttcaagcaga agaaaattat
     6661 ttctaaaaac tgctcctaat gttgacggct tgaatgtagt attgtttcat actcatggaa
     6721 ctgaggcctt tcaatcagct tatgactcaa agtacaggtc attggacgaa agtttaaata
     6781 ttttggggat agggacgaaa atttcaaaca gtctgcttaa ccaaggtatt aataatattc
     6841 acttaaaagg ctacaatgac tatccggatt acaataaatc ctatgcaaat tcaaactatg
     6901 cagtcaggca ggtattatcc gacagcaaaa aaaatgtatt aatcgatctg catcgtgacg
     6961 gtgctgaaga aaattccaat tatgaagcaa ttctgtcgga agttaaaact atcgagatta
     7021 acggcaaaaa tgcagcaacc tgcaccttgg tgacagggaa taaaaataaa aatgccggag
     7081 cgctgaagga aaatgcggga aggctgtatg atatagcata tgaaatgtat ccggggcttt
     7141 tcaggaaaat aattataagg gaaggtgcat attttaatca atatttatct gattattctt
     7201 tgcttatcga agtaggatgc accttgaata catatgaaga aattgaatat acggctgagc
     7261 tgcttacaga agtattatat gaatatatta atgaaattca ataattgcag ataaataata
     7321 attttacatt tacataaaca tttgatataa tacccttagt ttaaatttag gagggttcat
     7381 ttttttggac agacaaaaat acataagaaa cttttcgatt atcgctcata tagatcatgg
     7441 gaaatcaaca ctggcagaca ggttaataga aaacaccggg cttcttacac acagagaaat
     7501 gcaggaccag gtactggaca atatggacct ggaaagggaa agagggatta caataaagct
     7561 tcaaaccatg agcttattgt acaaggcaca ggacggacac agctataccc tgaacttaat
     7621 tgatactccg ggacatgtgg acttcagcta tgaggtttca aggagtctgg ctgcatgcga
     7681 aggagctgta ctggttgtgg atgctgctca gggtattgaa gcacaaacat tggctaatgt
     7741 gtacctggca ttggatcaag accttgaaat agttcccgtt ataaataaaa ttgatttgat
     7801 aagcgcaaga ccagatgaaa ttaaacaaga aattgaggat gtaataggtc ttgactgctc
     7861 tgatgccccg ctgatttcag ctaaggaaaa tataaatatc gaccaggttt tagaagcaat
     7921 agtaaaaaag attccgccgc ctgaaggcga cgaagatgct cctttaaaag ctttggtatt
     7981 tgattcttat tatgacagct acagaggtgt ggtggcatat ataagaatca aagaaggcca
     8041 ggttaaaaag ggagataaaa taaaaatgat gtcaacaggc aaaacctttg atgtcacaga
     8101 aacaggcata atttcaccct tccagaaacc cgtatcctgt ctttattcgg gagatgtggg
     8161 ttatttgtgt gccagcatga aggaggtaaa aagctgtcag gtgggagata caataactaa
     8221 cagtgcaaat cctgcaaaag accctctccc tggttataaa aaggttacat ccatggtttt
     8281 ttgcggtatt tatcctgccg agggcgagga atacaacagt gtcagggatg ccctggaaaa
     8341 acttcaggta aatgatgctt ccttaacata tgagccggaa acatcggttg ctttgggatt
     8401 tgggtttaga tgcggattcc ttggacttct tcatatggaa ataattcagg aaagactgga
     8461 gagggagttt gatttaaata ttatagcaac aactccaagt gtaatataca aagtatataa
     8521 aaccgacggg caaatgatga tattgcaaaa ccctacaaat atgcctcctg tccaggaaat
     8581 cgaatatatg gaagaaccta tagttgccgc atcaataatg agtcctaccg aatatgtggg
     8641 agcaattatg gagctttgtc aaaagcgcag aggcacattt gtaaatatgg aatacatgga
     8701 ggcaacaaga gttatactcc attataaatt acctttgaat gaagtaattt atgatttctt
     8761 cgatgcatta aaatctaaaa caagaggata tgcttctttg gattatgagc ttgacagcta
     8821 tgtacagtct gaattggtga aaatggatat tttgataaac tctgagctgg tagatgcatt
     8881 ttcaataatt gttcatgaag aaaaggctta tgagagagga agagcaatag ttgaaaggct
     8941 gagggaagta attccgagac atttgtttgc tgtacctctt caggctgcaa taggaaataa
     9001 gattattgcc agagagaccg taaaagccct gagaaaagac gttcttgcaa aatgctacgg
     9061 cggagacatc acaagaaaga aaaagctatt ggagaagcaa aaagagggca agaagcgtat
     9121 gagacaaatt ggcatggtgg aagtgcccca ggaagcattc ctggcggtgc ttaaatttga
     9181 tgaagatt
//
LOCUS       DAOPUT010000058         9154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_7593_length_9154_cov_19.764809, whole genome shotgun sequence.
ACCESSION   DAOPUT010000058 DAOPUT010000000
VERSION     DAOPUT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7593_length_9154_cov_19.764809"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKK75_05935"
     CDS             complement(<1..693)
                     /locus_tag="PKK75_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNZ83173.1"
                     /translation="MKQFIKVFLFSLIIFSIIITTGIFTYSKFTSPEGIVQELPEGDD
                     LISDDIEEGDDLTPLERAMRDSNRVNALLLGFEGARSDTIMLASFDRKTKEANIISIP
                     RDTYYQRDGYAKYSEMQKMNAVYGSEDEGYKAVMEAVEEITGISVDRYVAVNYNGVRA
                     AVNAIGGVEVNVPIHMKYTDIYDDPPLYIDIGPGPQIIYGDKAMELLRFRSGDPGYPS
                     FPNGDLGRVEMQQ"
     gene            complement(716..1669)
                     /locus_tag="PKK75_05940"
     CDS             complement(716..1669)
                     /locus_tag="PKK75_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNZ83174.1"
                     /translation="MIKHFIKIFFASLLLISVVLSFTALGYIFLVDKDIAIGKIEFGN
                     DEKNGFAFYESSTFDYGTSLGKAASNMKRFNILLAGLENKRTDTIMLVSYDTENKTAD
                     IISLPRDTYLPRNADDEAELKKINAVYGQEGIEGLTLRVQGLLGIPVEKYAVIDYEAV
                     VACVDMIGGVQVDVPFHMEYTDIYDDPPLYIDIPEGVQVLDGEQSLKFLRFRKGYDNQ
                     DLGRIEAQQQFIKSFVKRSLGLQLPEVIKEAYSHIETNISVSDLLDLAGDLVGFSTDN
                     INTCILPGMETPLEGLSFYVPDENGIKNLIYSLYGLEISNN"
     gene            1879..2079
                     /locus_tag="PKK75_05945"
     CDS             1879..2079
                     /locus_tag="PKK75_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNZ83175.1"
                     /translation="MKTGTVKWFDSKKGFGFISCSDGKDVFVHFSAIISDGFKTLEEG
                     QKVNFDIVEGQKGPQASNVTVA"
     gene            2227..2427
                     /locus_tag="PKK75_05950"
     CDS             2227..2427
                     /locus_tag="PKK75_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009791059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNZ83176.1"
                     /translation="MKTGTVKWFDSAKGFGFITSDEGNDVFVHFSAILSNGFKTLEEG
                     QKVKFEVVEGARGPQAANVEVI"
     gene            complement(2473..3339)
                     /gene="rbsK"
                     /locus_tag="PKK75_05955"
     CDS             complement(2473..3339)
                     /gene="rbsK"
                     /locus_tag="PKK75_05955"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016113116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNZ83177.1"
                     /translation="MGKVVVVGSINVDMVFTSDIRPKAGETVLGRTFSIIPGGKGANQ
                     AVAAAKLGADTYMVGCVGNDSNGDFSLKNLQNMNVNTEAVKVVEYAPTGVANIVVAEN
                     DNSIIVISGANFKVNKELIDNNKNLILSADIVLLQLEIPMEAVEHVVKICAENKVKVL
                     LNPAPAARLRKSIIEKASYLTPNEHELKLIFDSRASDEVLKNYPNKLIVTMGSKGVKY
                     FDGNEIKEIPSNKVDVVDTTGAGDTFCGGLAAALVRGYNMEDAIRFANKAASYAVTKL
                     GAQSGMPSLGDL"
     gene            complement(3387..4337)
                     /locus_tag="PKK75_05960"
     CDS             complement(3387..4337)
                     /locus_tag="PKK75_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNZ83178.1"
                     /translation="MKKRNIIIDCDPGIDDIIAISLAIANEDKFNILGITTVAGNQTI
                     EKVTLNALKVLSYYNRNIKTVMGQKRPLIREKSAVSNVHGENGMGDCHLPEPSLKLYS
                     DNAVTFLRDTILSQKEKVTLVPVGPLTNIALLIKVFPEVRENIELISIMGGSTAGGNR
                     TPCAEFNVWADPEAARIVFDSRLPIVMSGLNVTHSTGLYKDDVEKLAESTGKATGLIG
                     KMLQFYFKGDHVKGGTFSPIHDACALMYLLYPELYKSRHMQVLVDCSEELNRGNTVAD
                     IRDWIKYDSTYPRVLTNVDTEKFREILLNDLYKLDGMIQG"
     gene            complement(4606..5526)
                     /locus_tag="PKK75_05965"
     CDS             complement(4606..5526)
                     /locus_tag="PKK75_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83179.1"
                     /translation="MIIEAVFSPEFLNATLRATTPILFAALASCIASKSGVINMALEG
                     IMLFTALFGVIFSAAFQSAWLGLLATMAVGGFIGFFLAFFVLRLKTDEILAAIAINLI
                     ANGATILILLYVSGDRGISSSIPSLIIPRVTLPIIKDIPFLGQVLSNQNILTYVSLIM
                     VAVVHIFLYRTPLGLKIRAVGENKDAAESVGISSDKIRYISLTLSGVLASMGGFFLSG
                     GYMTKFTIGMSAGRGFIALAAGAMGGNTPIGSFLVSLLFGAAQALSNILQLGAWPHEL
                     VQMLPYVTTLIGLGIYSYSRMKKRQRLTGR"
     gene            complement(5528..6595)
                     /locus_tag="PKK75_05970"
     CDS             complement(5528..6595)
                     /locus_tag="PKK75_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83180.1"
                     /translation="MNSSTKLNILRTLLAVFIALLISFGIIFLTSDEPVKAIVQLLTG
                     PLQSQRRFGNVIEAMIPLIFTGVGISIMFAANEINLAGEGAFHLGGLAATYFALNTAL
                     PPVISPIATILIAGLCGMIVTGIPALMKIKTESDVLVSSLMMNYLVLYFSTYVLSNIL
                     RDPAAGAVVSFAVPSVTQLGTIFNGTRIHVGLFIALAAAAIGYIFLYKTKLGYEIRVT
                     GENREFARYSGINVVKITLVSQLFGGAIFGIGGGVELLGMYNRFTWTSLLGYGWDAII
                     ITTLAKKNPIYVPLAAFFLAYLRTGASIMSRSTDVVTEIVTITQGIIILLVVAEQFLS
                     KYKHKIISKEAKAALVEKEGE"
     gene            complement(6585..>7535)
                     /locus_tag="PKK75_05975"
     CDS             complement(6585..>7535)
                     /locus_tag="PKK75_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ83181.1"
                     /translation="KLDEIKEICDRATIMRNGRSKGTYLVKDITTAEMSRLMVGRDVV
                     TTFDKKPAELKDTVLKVRNLSVTGTTGIKRVDDLSFDVKKGEIFGIAGVEGNGQGQLV
                     EVLTGLSKKYKGQIKVFDQDAKKYSIRELRDLGMAHIPEDRMTYGCARNMNIMDNIFT
                     NQYHKTEYSGKVLLKNRKIENRVNELIKEYRVKTKSYRQSVGMLSGGNIQKVVVAREF
                     STSPAIIIANQPTRGIDVGAASFIRQRILDFRDSGCAIILISADLNEVLELSDRLAVI
                     YKGQFSGVFNNVKNVTEVELGKYMLGIKKDDRLEGIIYEQ"
     assembly_gap    7536..7635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7636..7982)
                     /locus_tag="PKK75_05980"
     CDS             complement(<7636..7982)
                     /locus_tag="PKK75_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ83182.1"
                     /translation="MGDKVLQMNNIMKIYPNGVVAVEDVTFELEKGEIHALLGENGAG
                     KSTLMKVLFGIESPEQGEIILNGQKENIKSPLDAIKKGIGMVHQHFMLVPSLTVAENI
                     VLGMEPKKNKLFI"
     gene            complement(8054..9136)
                     /locus_tag="PKK75_05985"
     CDS             complement(8054..9136)
                     /locus_tag="PKK75_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ83183.1"
                     /translation="MAAMVFSLAACAQQPGTKEGEVYKAALLLNGTLGDKSFFDSAND
                     GLVRLKEDLGADVFDFEVEQMGATAADEPKWAPTLLDFCETGEYDVIIIGTWQMIEGL
                     EDAAKQFPDQKFIFFDETYAFANGEKNVYNVLYKQNEVSFLVGAAAAMMTTDERLPMV
                     DPKTKSIGFLGGMENPIINDFLLGYIQGAKHVEPDIKVAIGYVGDFWDSAKGKDIALV
                     QYQSGGVDVGYNVAGAAGLGQIEAAEVAKRYAFGVDSDQAALLPDKAEFIPSSALKNV
                     GNSLMRAIKLDLEGKLAYGSAESLGFKEGGVNLVKDAHYEEMLPEDIRTRIDELEKDI
                     IDGKIVVDTALGKTTEEVEAIKESVQ"
BASE COUNT         2485 a         2047 c         1579 g         2943 t
ORIGIN      
        1 ctgctgcatt tccacccttc ctaaatctcc gttaggaaat gaaggatagc ccgggtctcc
       61 cgatctgaat ctgagaagct ccattgcctt gtcgccgtat ataatctggg gtcccggtcc
      121 tatatctata taaagaggag ggtcatcata tatatccgta tatttcatat gtatgggaac
      181 atttacttcc acaccgccga tagcattaac ggctgccctt acaccgttgt aattaacagc
      241 aacatacctg tccacagaaa tgcctgttat ttcttccacc gcttccatta cagccttata
      301 gccttcatct tcgctgccgt agacagcatt cattttttgc atttctgaat atttggcata
      361 tccgtctctt tggtaatagg tatctcttgg aattgatata atgtttgctt ccttggtttt
      421 tctgtcaaag ctggcaagca ttattgtgtc tgatcttgca ccttcaaacc ctaaaagcag
      481 ggcattcacc ctattgctgt cccgcattgc tctttccagg ggtgtcaaat catcaccttc
      541 ttcaatatca tctgagatga ggtcatctcc ctccggcagt tcctgtacga ttccttcagg
      601 ggatgtaaat tttgaatatg taaatatacc tgtagttatt ataatagaaa atattattaa
      661 agaaaagaga aataccttaa taaactgttt catataattc cgcctttcag aaaaattaat
      721 tattgcttat ttctaaacca tataatgaat aaatcaaatt ttttatgcca ttttcgtccg
      781 gtacgtaaaa ggataaacct tccagaggcg tttccatgcc gggaagtatg cacgtgttga
      841 tattatctgt tgaaaatcca accaagtccc ctgccaggtc aagcaggtcg gaaacactaa
      901 tgtttgtttc aatgtgcgaa taagcttctt ttataacttc gggcagctgt aatcccaaag
      961 agcgttttac aaaagatttt ataaattgct gctgtgcttc tattctcccc aaatcctggt
     1021 tgtcatatcc ctttctgaac cttaaaaatt tcagtgactg ctctccgtca agaacctgaa
     1081 caccttcagg tatatctatg tacaatggag ggtcatcgta aatgtccgtg tattccatgt
     1141 gaaaaggaac atccacctga actccgccta tcatgtcaac gcatgcaact accgcttcat
     1201 aatcaataac cgcatatttt tccacaggaa tccccaaaag cccctgaact ctcaatgtta
     1261 gaccttcaat tccttcctgg ccgtatactg cattgatttt cttaagctct gcttcatcat
     1321 ctgcatttct tggaagatat gtatctctgg gcagtgaaat gatatctgct gttttgtttt
     1381 ctgtatcata gcttactaac attatggtat cggttctttt attttccagt cctgccaaaa
     1441 gaatgttaaa ccttttcata ttagacgcag ccttgcccag ggaagttcca taatcaaagg
     1501 tggaggattc ataaaatgca aatccgttct tttcatcatt cccaaattca atttttccta
     1561 tagcaatatc cttgtcgacc aaaaaaatat aacctaaggc tgtaaagctt aatacaactg
     1621 atataagcag taaggatgca aaaaatatct ttataaaatg tttaatcatg ctacaccttt
     1681 ttaataatta tcctagacaa ttataacaaa gtgcttattg tttttcaatt ggaataaaaa
     1741 attattttcc gcaaattttt aataagactt ccattttttt aatttgattt tattaattag
     1801 aaaaataata tgttgcaata tacaaagaaa actgttacaa tacaagtatc ttttttaata
     1861 tcggaggatt tttttaaaat gaaaacaggc acagtaaaat ggttcgattc caaaaagggt
     1921 tttggattta tatcctgcag tgacggaaag gatgtatttg tacatttttc cgcaataata
     1981 tcagatggat tcaaaacatt ggaagaaggg caaaaagtaa actttgacat agttgaaggt
     2041 caaaaaggac ctcaggctag caatgttact gtagcatagt agtaaattgt ttttcgaaat
     2101 aatacgttaa tatagatatg gtttggcatt acttatatta atattagtat ttgaaggaaa
     2161 cgtgataaga actacatgtg tagttctttt tcatatatta tttttataaa aacatggagg
     2221 agtttaatga aaacaggtac agtaaaatgg tttgattctg cgaaaggttt tggatttatt
     2281 acaagcgacg aaggaaatga tgtgtttgtg catttttccg caatcttatc caatggtttt
     2341 aaaactttgg aagaaggaca aaaagttaaa tttgaagtag ttgaaggagc aagaggtcct
     2401 caggctgcta atgttgaagt tatctaactt atgcgcagaa gcacctattc actgaatagg
     2461 tgcttttttt tattacagat cacctaatga aggcatcccg ctttgggctc ctagttttgt
     2521 tactgcatat gaagctgcct tgtttgcaaa cctgattgca tcttccatgt tgtaacctct
     2581 aaccaatgca gcagccaaac ctccgcagaa tgtatctcct gctccggtag tatccacaac
     2641 atccaccttg tttgaaggaa tttcttttat ttcattcccg tcaaagtatt ttactccctt
     2701 tgaacccata gtaacaatta atttattggg ataattcttc agaacttcat cagaagcccg
     2761 actgtcgaat attaatttca attcgtgttc attcggtgtc aggtatgatg ctttttcaat
     2821 tatagacttt cttaacctgg ctgcaggagc agggtttaac aggaccttca ccttgttttc
     2881 agcacatatt ttcaccacat gttcaacagc ttccatgggt atttccagct ggaggagcac
     2941 aatgtccgct gacaatatca ggtttttatt gttgtcaatc aattctttgt tgactttaaa
     3001 attagcacct gaaattacta taatgctgtt atcattttct gccacaacaa tatttgctac
     3061 tcccgtggga gcatattcaa caacttttac tgcctccgtg ttaacattca tgttttgaag
     3121 gttcttaaga gaaaaatcac cgtttgaatc attccccaca cagccaacca tgtacgtatc
     3181 cgctcccaat ttagcagcag ccacagcttg atttgcaccc ttgcctcccg ggataatgga
     3241 aaatgtccgg cctagaacag tttctcctgc cttaggtctt atatcggatg taaaaaccat
     3301 atctacatta atgcttccaa ccacaactac ttttcccata atacctcctt atcgctgcga
     3361 cggggaaatt ccttttctat aaagatttaa ccctgtatca taccatccag cttatataaa
     3421 tcattaagca gtatctctct gaatttttcc gtatccacat ttgtcagaac tcttggatat
     3481 gtgctgtcat atttaatcca atctcttata tctgcaaccg tattgcccct gttcaactct
     3541 tcggagcaat ccacaagaac ctgcatatgc ctggacttat acaattcagg atataatagg
     3601 tacataagag cacaagcatc atgtatgggt gaaaaggtcc cgccttttac atggtcccct
     3661 ttaaaataaa attgaagcat ctttccaatt agtcctgttg ccttgccggt actttcagcc
     3721 agcttttcaa catcatcctt gtacaatcct gttgaatgtg taacattaag gccgctcatt
     3781 acaataggaa gccttgaatc aaaaacaatt cttgctgctt ccgggtcagc ccaaacattg
     3841 aattccgcac agggtgtccg gtttccccct gctgtggacc ctcccattat tgatatgagc
     3901 tcaatatttt cccttacctc aggaaaaacc tttataagca aagcaatatt ggtaagaggt
     3961 cctacaggca ccagggtgac tttttctttt tgagacagta ttgtgtctct taaaaatgtt
     4021 actgcattat cagagtaaag ctttaagctt ggctcaggca ggtggcagtc acccatgccg
     4081 ttttccccgt ggacattgct tactgcgctt ttttctctta ttaaaggtct tttttgtccc
     4141 atgactgttt taatgttcct gttgtaatat gacagaacct tcaatgcatt cagtgttact
     4201 ttttctattg tttgattacc tgcgacggtg gtgattccaa gaatattaaa tttgtcctcg
     4261 ttggcaatcg ccaatgatat tgcaattatg tcatcaatac ccggatcaca gtctatgatt
     4321 atatttcgtt ttttcatatt ttcccctttc ccgtagtcag ttaggtcatc ttacagtctc
     4381 gttgcacaat tttgtttgct ccttgtgtcg cacaaaattg gcactcgttg cgaatgacct
     4441 accatccatc tgttcttcgc tttagctcga acatatgggg ttaggtcatc ttacagtctc
     4501 gttgcacaat tttgtttgct ccttgtgtcg cacaaaattg gcactcgttg cgaatgacct
     4561 accatccatc tgttcttcgc tttagctcga acatatgggg ttaggtcatc ttcccgtaag
     4621 acgctgtctt tttttcattc ttgagtagct gtatattcca agacctatga gtgttgttac
     4681 gtatggcagc atttgtacca attcatgagg ccaggctcca agctgcaaaa tgtttgaaag
     4741 cgcctgtgct gcaccgaaga gcaaggatac cagaaatgag cctatgggtg tatttcctcc
     4801 catggcaccg gcagccagtg caataaaacc tcttcctgcg ctcatcccta tggtaaattt
     4861 ggtcatgtat cctccggaaa ggaaaaaacc tcccattgaa gcaagaacac cgcttaaggt
     4921 taatgatatg tagcggattt tgtcagaact gatacccaca gattcagcag cgtctttgtt
     4981 ttctccgact gctcttattt taagccccaa aggagtccgg tagaggaaaa tatgaacgac
     5041 agccaccatt atcagtgaaa cataagtcag aatattctgg ttggataaaa cctgacccaa
     5101 aaatggaata tcttttataa tcggcagggt tacccttggt attattaagc ttggaattga
     5161 agaagaaata cccctgtctc ccgatacgta cagaagtatg agtatggttg ccccgttggc
     5221 aataaggttt attgcaattg ctgcaagtat ttcatctgtt ttcagtctga gcacaaaaaa
     5281 tgctaggaag aatcctatga aacctcccac tgccatagta gccaaaagtc ccagccatgc
     5341 actttggaaa gccgcactga atattactcc aaaaagtgcc gtgaaaagca taatgccttc
     5401 caatgccata ttgataactc cggatttgga tgctatacaa gaagcaagtg ctgcaaataa
     5461 tataggcgtt gttgccctta aggttgcatt taaaaattcc ggtgaaaaaa ccgcttcaat
     5521 tatcatatta ttcaccttcc ttttcaacta aggctgcctt tgcctcttta gaaataattt
     5581 tgtgtttata cttgcttaag aattgctctg ccacaaccag gaggattatt atgccttgtg
     5641 ttattgtaac tatttctgta accacatccg tggaccggga cataattgac gcccccgttc
     5701 gcagatatgc aaggaaaaat gcagcaaggg gaacatagat ggggtttttc ttagctaatg
     5761 ttgtaataat tatagcatcc catccataac ctaaaagtga tgtccatgtg aaacggttat
     5821 acattccaag aagttccact cctccgccga tgccaaaaat agctcctccg aacaactgag
     5881 ataccaatgt tatttttacc acatttattc cggaatacct ggcaaactct ctgttttctc
     5941 ctgttacacg tatttcatag cctaattttg tcttatacaa aaaaatatat ccaatcgctg
     6001 ccgccgctaa tgctataaac aagcctacgt gaattcttgt tccgttgaaa attgtcccga
     6061 gctgagtaac tgaaggcacg gcaaatgaaa ctacggcacc tgctgcgggg tctcttaaaa
     6121 tattcgataa aacataagtt gaaaagtata gaaccaaata attcatcatc agggatgaaa
     6181 ccaacacatc tgattctgtt tttattttca tcaatgcagg aattcccgtt actatcatgc
     6241 cgcataatcc tgcaataaga attgttgcta tggggcttat taccggcggc aaggcagtgt
     6301 ttaaagcaaa gtatgttgcc gccaggcctc ccagatgaaa tgctccttcg ccagccaggt
     6361 ttatttcatt ggcggcaaac atgatgctta ttcccacacc tgtaaagatt aagggtatca
     6421 tcgcctctat gacattgccg aaacgcctct ggctctgcag gggacctgtg agaagctgaa
     6481 caatcgcttt gacaggctca tcgcttgtaa gaaagattat tccgaatgat attaataaag
     6541 caatgaatac agccagcagg gttctaagta tgtttaattt tgtactactg ttcatatatt
     6601 ataccctcca gtctgtcatc cttttttata cccagcatgt atttgccaag ttcaacttca
     6661 gttacgtttt taacattgtt aaatacacct gaaaactgtc ctttgtaaat aacagcaagt
     6721 ctgtcgctta attccagcac ctcgttcaaa tcggcagata ttaaaattat ggcacagcct
     6781 gaatctctga aatcaaggat tctttgcctt ataaaggagg ctgctcccac atcgatgcct
     6841 cttgtaggct gattagcaat tattatggca gggctagtgg aaaactccct ggcaactaca
     6901 accttctgaa tatttccccc tgaaagcatg ccaacgcttt gcctgtagga cttggtcttt
     6961 accctgtatt ctttaatcag ctcattgact ctgttttcaa tttttctgtt cttaagaagc
     7021 acctttccgg aatactcggt cttgtgatac tggtttgtaa atatgttatc cattatattc
     7081 atgttccttg cacagccgta ggtcattctg tcttccggta tgtgagccat tcccaagtct
     7141 ctcagctccc tgattgaata tttctttgca tcctggtcaa atacttttat ttgacccttg
     7201 tacttttttg aaaggcctgt aagaacttcc accaattgtc cctggccgtt tccttccacc
     7261 cctgctatgc cgaaaatttc tcctttcttt acatcgaagg acaaatcatc aactcttttt
     7321 attcctgtag tacctgtaac actcaggttc ctgaccttta acacagtgtc ctttaattct
     7381 gccggttttt tatcaaatgt agtgacaaca tctcttccta ccatcagcct tgacatttca
     7441 gcagttgtaa tatcctttac caggtatgta cccttgcttc ttccgtttcg cattatggtt
     7501 gccctgtcgc atatttcttt gatttcatcc agcttnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnntctat gaaaagtttg ttcttttttg gctccattcc taatacaatg
     7681 ttctctgcaa cagtcaatga aggtacaagc ataaaatgct ggtgcaccat tcctatgcct
     7741 ttttttatgg cgtccagggg cgactttata ttttcttttt gcccgtttag aattatttct
     7801 ccctgctccg gcgattcaat accgaacaat accttcatca aggtggattt tcctgctccg
     7861 ttttccccta acagggcatg aatttcaccc ttttccaatt caaatgtaac atcttcaaca
     7921 gcaactaccc catttgggta aatcttcata atgttattca tctgaagtac tttgtcaccc
     7981 attttatact gctacctccg ctaagtaaag taggaggacg gcgttgattc caacaacccc
     8041 gtcccccttc cgcttattgt actgattctt ttattgcttc aacttcttcg gtggttttgc
     8101 ccaaggcagt atcaacaact atcttcccgt ctatgatatc tttttccaat tcgtcgattc
     8161 tcgtcctgat atcttccggc agcatttctt catagtgagc atcctttacc agattaactc
     8221 ccccttcttt aaagccaagg gattctgcac tgccgtatgc caatttgcct tccaggtcaa
     8281 gtttaattgc tctcataagc gagttgccta catttttcag tgctgatgaa ggaataaatt
     8341 ctgccttgtc aggcagtagt gccgcctggt cagagtcaac tccaaatgca tatctctttg
     8401 caacttcagc tgcctcaatc tgtcctaagc ctgcggcccc tgccacattg tacccaacat
     8461 caacgccgcc gctttggtac tgaaccagag ctatatcctt gccttttgct gaatcccaga
     8521 aatctccaac ataacctatt gcaaccttga tatccggctc aacatgtttg gctccctgta
     8581 tataacccag caagaaatca ttgatgatag gattttccat tccccccagg aaacctatag
     8641 atttagtttt aggatcaacc ataggcagtc tttcgtcggt tgtcatcatt gctgctgctg
     8701 cacctaccag gaaggaaact tcgttttgtt tatacaatac attgtaaaca tttttctctc
     8761 catttgcaaa ggcataggtt tcatcaaaga atataaattt ttgatccggg aattgttttg
     8821 ctgcatcttc aagtccttct atcatctgcc atgtaccgat aattattaca tcgtattccc
     8881 ctgtttcaca gaaatcaagc aaagtcggcg cccatttagg ctcatctgca gctgttgctc
     8941 ccatttgttc aacctcaaaa tcaaatacat ccgcacccag gtcttccttc aatctcacaa
     9001 gtccgtcgtt ggcagagtca aagaacgact tgtctccaag tgttccgttt aaaagaaggg
     9061 ctgccttata aacttcgcct tctttcgttc ccggctgctg tgcacatgct gccagggaaa
     9121 aaaccatagc agcaattaat aatagtgata gtgc
//
LOCUS       DAOPUT010000059         9021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_7725_length_9021_cov_18.927392, whole genome shotgun sequence.
ACCESSION   DAOPUT010000059 DAOPUT010000000
VERSION     DAOPUT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7725_length_9021_cov_18.927392"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKK75_05990"
     CDS             <1..252
                     /locus_tag="PKK75_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83184.1"
                     /translation="GRFSTWPVPVAMMNTVASTEYIIEWINGNVGDELDVDVLEEKMA
                     EYATLPVATSPYTEEGLEIPHFRLIMMDFLTYGEEHIIN"
     gene            368..1963
                     /locus_tag="PKK75_05995"
     CDS             368..1963
                     /locus_tag="PKK75_05995"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNZ83185.1"
                     /translation="MSDNLLSLKHVSKEYSGNKVLKDINIELKKGEIHALIGENGAGK
                     STLMNILFGMSVIHTTGGFEGTIEIEGKEADIKSPHDAMNYGIGMVHQEFMLVPGFTI
                     MENIKLNREITKPNLVSPVFGKRLETLDIKKMNMDTRQALDTLDMSIEEYVQVAGLPV
                     GHMQFIEIAREIDKTGLKILVFDEPTAVLAESEAANLLNAMKKLAQKGIGILFISHRL
                     DEIIDVADVVTVLRDGEHVVTKNVEDTNKYEIAQLMVGRSITIDREEGTNKNIVDETI
                     LDIKDLHVAMPGERVKGVNLNVKKGEIVGIGGLAGQGKLGIANGIMGIFPAKGEVKFK
                     GKKSALNDPKSAIKSGLAFVSEDRKGVGLLLDESIESNIAFSAMQINNEFLRNSGMIK
                     LKDSKKIRDHANKMIKDLDIRCTSSMQRVGSLSGGNQQKVCVARALTLSPELLLVSEP
                     TRGIDIGAKKLVLDLLKQLNNEGMTIIMTSSELAELRSICDRILIICEGKVEGVLPPD
                     APDAEFGLMMSGSRHIEGREVNG"
     gene            1956..3029
                     /locus_tag="PKK75_06000"
     CDS             1956..3029
                     /locus_tag="PKK75_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83186.1"
                     /translation="MVNVSKKTTGFNDIVKKIGFPTIFIGGFWILLLIAGGALGISIN
                     TLLSDTIKRAGMNGILVLAMVPSIQSGTGPNFALPVGIVCGLFATVWAIEFGFTGWAW
                     LFVSIAFSIPLSVVVGYLYGRLLNAVKGSEMTIATYTGFSIVAAMCLAWLMIPFKSRN
                     MGWFIGKGLRETIQLDVIGGDKILSEFLSFTIGGEKGIIIPTGMLLIFFLCCFIVWMY
                     FRSKSGIAISAGGINPKFAKSSGIDIDKGRIKANILSTIMGAIGIIVYSQGYGYTQLY
                     TAPLMMAFPAVAAVLIGGASAQRAKVSHVIIGVIIYQGLFTTALPVANQLFEGTDLSE
                     ILRMVIQNGIILYALTQVKGGDN"
     gene            3029..4330
                     /locus_tag="PKK75_06005"
     CDS             3029..4330
                     /locus_tag="PKK75_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83187.1"
                     /translation="MNNENISERLNSSKVTTWLADNIVSLIFVAFTLFGFLVSDGVTM
                     NFFLTELSSRFFRNAFLVLSLIIPVVAGLGLNFGIVVGAMAGQVAIAIVLYFGWGGLN
                     GLLLTMLIALPIATLFGYLTGILYNKTRGQEMIASLMVGYFANGIYQFLFLFVVGVII
                     KVPKTHPMIKPDGVGVRMTVDMGAYDKGGLKYALDKIWEIPFMWAILAIFAAILLLQL
                     WKLIRNKKNPVLYRYNKTMTVINIILSLVMIAVSIQAITTESSLMMVRKVPVFTGLLI
                     VALCIFNELILKTKLGQDFRSVGQSQHIAEVSGINVDKTRIIATIMSTVFAAWGQIMY
                     LQNMGTLNTYGAHTQIGMFSVASLLVGGASTQKAGVKHAILGTLLFNSMFIMSPEIGL
                     SLFGNALLGEYFRTFMVYGVIGLALGLHVWRTNKKGKATLN"
     gene            4453..5484
                     /gene="tsaD"
                     /locus_tag="PKK75_06010"
     CDS             4453..5484
                     /gene="tsaD"
                     /locus_tag="PKK75_06010"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNZ83188.1"
                     /translation="MKENTKDKTLILAIETSCDETAASVVENGRNVLSNIISSQIEIH
                     KEFGGVVPEVASRKHIESINEVVSKAMNEANAEPGDIDGIAVTYGPGLVGALLVGINF
                     AKGLSYAWEKPLIGVNHIEGHINANYIEYKELKPPFLCLVVSGGHTHLVHVKDYGMYK
                     ILGMTRDDAAGEAFDKVARAIGLGYPGGPLIDQAARRGNKDKIKFPRAIIDDETLDFS
                     FSGLKSAVINYLHNSKQKDETVNVEDVAASFQEAVVDVLVMKTIKAAKQEGLKTIALA
                     GGVASNSRLRERFQEECLGHNYIFMKPSPILCTDNAAMIGCAGYYKYLGEKFADSSLN
                     AMPNLELAN"
     gene            complement(5593..7509)
                     /locus_tag="PKK75_06015"
     CDS             complement(5593..7509)
                     /locus_tag="PKK75_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNZ83189.1"
                     /translation="MIVLSCNNVSKAYVVENIIEDISFTINDNEKVGLIGLNGAGKTT
                     LFNILTGNLEPDSGEIFLAKGKKLGYLRQNTIIESPMSIMDEMLLIFEEVIKIEEKLH
                     DLSHQIGSFAEDENPAELDTLMNTYSKLSELFVEADGYSFRSIIKGVLKGLGFSEDEF
                     NKPINVLSGGEKSRVMLAKLLLQKADILLLDEPTNHLDIEAISWLEKYIKNFKGEVII
                     ISHDRYFLDNTVNRIFLMENKSLKTYRGNYSEYMKRRKIEIEQTMKEFDEYEREIERQ
                     EEMIRRLHSYGSKRNIRQAFSRQKALDKIKKLDRPAVENRKVKLRFTPKITSGRDVLS
                     VADAAVSFGDRQILKNVNITVYKGEKVGIIGPNGIGKTTLLKIIAGVMNEYEGDITFG
                     HNVNIGYFDQEQTGLNNESTVIDEIWDENPDLNYYQIRRYLAQFLFYGDDMYKLVDDL
                     SGGEKGRLSLLKLMTSNANFLLLDEPTNHLDIDSKEVLEDSLIDYDGTILVVSHDRYF
                     LNKVVNKIYDLSEEGAFEYLGDYDYYVEKKAELDETEDDETLNKTKTQINAEKKKERE
                     LIRDRQQREKKLKEIEEQIEMHENEIHELETKLCQPSTYSDNFLIINLNEKLNIYKKE
                     LKDLYLLWEKTLEN"
     gene            7679..>9021
                     /gene="glmM"
                     /locus_tag="PKK75_06020"
     CDS             7679..>9021
                     /gene="glmM"
                     /locus_tag="PKK75_06020"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNZ83190.1"
                     /translation="MGRLFGTDGVRGIANKDLNPELAFKLGRTGGYFLTKGIKRPKMV
                     VGIDTRISGDMLEGALSSGLNSAGIDVLYLGIISTPAVACMIKILDADGGVMITASHN
                     PVEYNGIKFFNKDGLKLTDEMEDSIEEYILNNIDIDYYPVSGDLGRKIRVENPIRKYM
                     DFLKGTIDIDLEGLKIAVDCGNGAAYKSAPQLLHELGAQVYVIHNDPNGININVNCGS
                     TKPEEIQRLVLETGADAGLSFDGDADRLIASDENGNIVDGDHVLAICGTCMKEKGKLK
                     NNTIVGTVMTNLGLDICLKKNNIDIVKTNVGDRYVLEEMLKNGYSLGGEQSGHIIFLD
                     YNTTGDGLLTAIQLLSIMKQKNSKLSKLSSIMKNMPQVLVNARVSDEKKNNYLEDETI
                     KNKIAEIEKHFDKNGRVLIRSSGTEPLVRVMIEGEDQEEINFHAAQLAKLIEERL"
BASE COUNT         2904 a         1594 c         1860 g         2663 t
ORIGIN      
        1 ggaagattct ctacatggcc ggtacctgtt gctatgatga atacagtagc ttccacagaa
       61 tatataattg aatggattaa cggtaacgtt ggcgatgagc ttgacgtaga tgtacttgaa
      121 gaaaaaatgg ctgaatatgc tacgcttcct gttgctacaa gtccatacac agaagaagga
      181 ttggaaattc ctcatttcag attgattatg atggacttct taacttacgg agaagaacac
      241 attattaatt aatccatata acataattaa ataaactaag caattaagga atgtttcaga
      301 atttcttatt aggaacttct gattcattcc tttgctaatt agtaaggtca tacaagagga
      361 ggtagtaatg agtgataacc ttctaagctt aaagcatgtt tcaaaagaat attccggaaa
      421 caaagtactg aaggatataa atattgagct taaaaaaggt gagatccacg cactgatagg
      481 tgaaaacggg gcaggtaaat ccacgttgat gaatattctc tttggaatgt cggtgattca
      541 cacaaccggc ggatttgaag gaaccataga aattgaaggc aaagaggcgg atatcaaaag
      601 tccccatgat gccatgaact atgggatagg catggtgcac caggaattta tgctagttcc
      661 cgggtttacc ataatggaaa acattaagct caacagagaa atcacaaaac ccaacctggt
      721 gagccctgtg tttggaaaac ggcttgaaac attagatata aagaaaatga acatggatac
      781 aagacaagcc ctggacacat tggacatgag tatagaagaa tatgtacagg tggcaggact
      841 tccggtaggt catatgcaat ttattgaaat tgcaagggaa attgataaaa caggattaaa
      901 aatcctggtt tttgatgagc ctacagcggt tttagctgaa tcagaagcgg cgaatcttct
      961 aaatgcaatg aaaaagctcg cccagaaggg aatcggtatt ttatttataa gtcacagact
     1021 tgatgaaatt atagatgtgg ctgatgtggt taccgtttta cgggatggag agcatgtagt
     1081 tacaaaaaat gtagaagaca ccaataaata tgaaatagca caattgatgg tgggacgaag
     1141 catcaccata gacagagaag aaggcacgaa caaaaatatt gttgatgaaa caatattgga
     1201 tataaaagat ttacatgtag caatgcccgg tgaaagagta aagggtgtta acttaaatgt
     1261 taaaaaaggt gaaatagtag gcataggcgg acttgcaggc caggggaaat taggaattgc
     1321 caacggcata atgggaattt tccctgcaaa aggagaggtt aaattcaagg gcaaaaaatc
     1381 agctttgaat gacccgaaat ctgcaattaa atcaggcctt gcctttgtca gcgaagacag
     1441 aaaaggcgtc ggtctcttat tggatgaatc aatagaatca aatattgcat tttcagccat
     1501 gcagattaac aatgaatttt taagaaattc cggtatgatt aaactgaagg attcaaaaaa
     1561 aatcagagac catgctaata aaatgataaa agaccttgat ataagatgca cctcctccat
     1621 gcagagagta ggaagcctaa gcggcggaaa ccagcaaaaa gtttgcgttg caagggcgtt
     1681 gacattgtct cccgagcttc ttctggtttc cgagcctaca agaggaattg atataggagc
     1741 taaaaaatta gttcttgact tattgaagca gttaaataat gaaggcatga ccataataat
     1801 gacttcatcg gaattggctg agcttagaag tatatgtgac agaattctta taatttgtga
     1861 aggcaaggtt gaaggggtgc ttcctcccga tgcaccggac gctgaatttg gtttgatgat
     1921 gtcaggcagc agacacattg aagggaggga agttaatggt taatgtaagc aaaaaaacaa
     1981 caggttttaa tgatattgtt aaaaaaatag gatttccaac aatcttcata ggaggattct
     2041 ggatactcct gcttattgcc ggaggagctt tgggaatatc catcaatact ttattaagcg
     2101 atacaattaa acgtgccggc atgaacggta tactggtttt agccatggtt ccttcaatac
     2161 aatccggaac cggtcccaac tttgcattgc cggtaggtat tgtatgcggt ttatttgcca
     2221 cagtatgggc tatagaattc gggtttaccg gatgggcatg gttgtttgtg tcaattgctt
     2281 tttccattcc attgtcagtt gtcgtaggat acctgtacgg caggctttta aatgccgtta
     2341 agggctctga aatgacaatt gcaacttata cgggtttttc aattgttgct gcaatgtgtc
     2401 ttgcatggct tatgataccc tttaaaagca gaaatatggg atggtttatt ggaaagggat
     2461 taagagaaac aattcagctt gatgtaatcg gcggagacaa aatattaagc gaatttctga
     2521 gtttcaccat aggcggtgaa aagggaataa taatcccgac aggaatgctt ttaatatttt
     2581 tcctgtgctg ttttatagta tggatgtatt tcaggtctaa atcaggtatt gccatatctg
     2641 caggaggtat aaatcctaaa tttgccaagt cttcaggaat tgatattgat aaaggcagga
     2701 taaaagcaaa tattttatca acaattatgg gagcaatagg aattatagta tattcacagg
     2761 gttacggcta tacacagctt tatactgcac ctcttatgat ggcatttcct gctgttgccg
     2821 ctgtgttaat cggcggagct tctgctcaaa gggcaaaggt atctcacgta ataataggag
     2881 ttataatata ccagggtctt tttaccacgg cgctgcccgt ggccaaccaa ttgtttgaag
     2941 gcacggactt atccgaaata ctgcgtatgg taatccaaaa cggtataatc ctttatgcgc
     3001 tgacacaggt taaaggaggc gataattaat gaataatgaa aatatatcag aaagattaaa
     3061 ttcatctaaa gttacaacat ggcttgcaga taatatagtg tcattaatat ttgttgcctt
     3121 tactctattt ggatttttag tgtcagacgg tgtaacaatg aacttttttc tgacagaatt
     3181 atcaagccgt ttcttcagga atgcatttct tgttttatct cttataatac ctgttgtggc
     3241 aggtctcggg cttaacttcg gtatagttgt aggtgccatg gcaggacagg ttgccattgc
     3301 cattgtatta tattttggat ggggaggtct taacggactt ctgctgacaa tgcttatagc
     3361 attgccgata gcaacattat ttggctacct tacaggaata ctttacaaca agacaagagg
     3421 ccaggagatg attgccagct taatggtggg ttattttgcc aacggtatat atcaattctt
     3481 attcttgttt gtagtaggtg taataataaa ggttccgaaa acccatccca tgattaagcc
     3541 tgatggcgta ggtgtaagaa tgacagttga catgggagca tatgacaagg gcggacttaa
     3601 atacgcactt gataaaattt gggaaatacc tttcatgtgg gctatattgg caatatttgc
     3661 agccattttg ctcttgcagt tatggaaatt aataagaaat aagaaaaatc ctgttttata
     3721 cagatacaac aaaacaatga ctgttataaa tattatctta agcctggtta tgattgcagt
     3781 aagcattcaa gcaattacaa cagaatcaag tctgatgatg gtcagaaagg ttccggtttt
     3841 tacgggatta ttgatagtag cattatgtat attcaatgaa ttgattttaa aaacaaagct
     3901 gggtcaggat ttcagaagtg taggtcagag ccagcacatt gctgaagttt caggcatcaa
     3961 tgttgataag acaagaatta tagcaacaat aatgtcaact gtgtttgctg catgggggca
     4021 aattatgtac ctgcagaata tgggtaccct taacacatac ggtgcacata cgcagatagg
     4081 aatgttttcg gttgcatccc ttttggttgg aggggcttcg actcaaaagg caggagtaaa
     4141 acatgcaatt ttgggaacac tcttgttcaa ctcaatgttt ataatgtctc ctgaaattgg
     4201 attgtccctg tttggcaatg cccttctggg agaatacttc agaactttca tggtatacgg
     4261 agttataggc cttgctttgg gactccacgt ctggagaaca aacaaaaaag gcaaagccac
     4321 tttgaactaa agatgataaa gggacggggg tgcttcatat tcgatgtcgc tcccgttctt
     4381 tttttcccct tgaatattaa aactcacagt agtataattt tattcagaaa atacggagga
     4441 aaattttttc aaatgaagga aaacacaaag gataaaactt taatacttgc catagaaaca
     4501 tcctgtgatg aaactgcagc ttcggtcgtt gagaacggaa gaaatgtttt atccaatatt
     4561 atatcatccc aaatagaaat acataaggaa ttcggaggtg tggtacctga ggttgcttca
     4621 agaaagcata ttgaaagtat caatgaagtg gtatcaaagg ctatgaatga agccaatgca
     4681 gagcccgggg acatagatgg aattgccgtt acctacggtc ccggtctggt gggggcactt
     4741 ttggttggta ttaattttgc aaaaggactt tcctatgcat gggaaaaacc cctgataggg
     4801 gttaatcata ttgaaggaca tataaatgct aattacattg aatataaaga gcttaaaccg
     4861 ccatttctct gcctggtggt ttcgggaggc catacacacc tggttcatgt aaaagattac
     4921 ggcatgtata aaatcctggg aatgaccagg gacgatgctg ccggagaagc atttgacaag
     4981 gttgcaagag caataggact gggatatccg ggaggacctt tgattgacca ggcggccagg
     5041 agagggaaca aggataaaat aaagtttcca agagcaataa tcgacgatga aacattggac
     5101 ttcagcttca gcggcttaaa atcagcagtt ataaattatc ttcataattc aaagcagaag
     5161 gatgaaaccg tcaatgtgga agatgtggca gcaagttttc aggaggctgt ggttgatgtg
     5221 cttgtgatga aaacaataaa agcggcaaaa caagaaggac tgaagaccat agcactggcg
     5281 ggaggggtcg cttccaacag ccgtctgaga gagagatttc aggaggaatg tttaggccat
     5341 aactatatat ttatgaagcc ctctccaatt ttatgcacag ataatgcagc catgatcgga
     5401 tgtgcaggat attataaata tttaggcgaa aaatttgccg actcatcctt aaatgcaatg
     5461 ccaaatttag aacttgcaaa ttgattaaca ccctattata aaaaagtatc aacagctgtt
     5521 gataaagttg ttgatactag taaaatagca aaaagttatt cacaacttat aaacaaagag
     5581 tggataattt ttttaatttt ctaaagtctt ctcccataac agatataaat cttttaattc
     5641 ctttttataa atgtttaatt tttcattcag attaataatt agaaaattgt cactatatgt
     5701 cgaaggttga cataatttgg tttctaattc atgaatttca ttttcatgca tctcaatctg
     5761 ctcttctatc tctttaagct ttttttccct ctgctgtctg tctcttatta gctctctttc
     5821 cttctttttt tccgcattta tctgtgtctt ggtcttattt aatgtttcat catcttcagt
     5881 ttcatcaagc tctgcttttt tttcaacata gtagtcataa tcacccagat attcaaatgc
     5941 tccctcttca gacaaatcat atattttatt tacaaccttg tttaaaaagt acctgtcatg
     6001 agaaacaaca agtattgttc catcatagtc aataagggaa tcctccagca cttccttgga
     6061 atcaatgtcc aaatgatttg taggctcatc cagcaataaa aaattagcgt ttgaagtcat
     6121 gagtttcaaa agcgaaagcc tccccttttc accgccgctt aaatcatcta ccagcttata
     6181 catatcatcc ccgtaaaaca aaaattgtgc aagatacctt cgaatctgat aataattcaa
     6241 atcagggttt tcatcccata tttcatcaat tacagtgctt tcattgttta agcctgtctg
     6301 ctcctggtcg aaatagccga tattgacatt atggccaaat gttatatccc cttcatactc
     6361 attcatcacg ccggcgataa ttttcagcag agttgtttta cctatgccgt taggtcctat
     6421 aatccctact ttttccccct tatacacggt tatgtttaca ttttttagaa tctgcctgtc
     6481 accaaaggac acagctgcat cagcaacact taatacatct ctgccgctgg tgattttcgg
     6541 cgtaaacctt aacttgacct ttctattctc aacagcaggc ctatcaagtt tttttatctt
     6601 atccaaagcc ttttgcctgc tgaaggcctg cctgatattt cgcttgctgc cgtatgaatg
     6661 gaggcggcgg atcatttctt cctgtctttc aatttctctt tcatattcgt caaattcctt
     6721 catcgtctgt tcaatttcaa ttttccttct tttcatatat tccgaataat tgcccctata
     6781 tgtttttaag gacttgtttt ccataagaaa aattctgtta actgtgttat caaggaaata
     6841 tctgtcgtga gaaataatta ttacttcacc cttaaaattc ttaatatatt tttcaagcca
     6901 ggaaatagct tcaatatcca agtgattggt aggctcatcc aataaaagaa tatctgcctt
     6961 ctgcaaaaga agttttgcca gcataaccct gcttttttcc ccgccgctta aaacatttat
     7021 cggtttgtta aattcatctt cagaaaaacc aagacctttt aaaacaccct taataatgct
     7081 tctgaaactg taaccgtcag cttcaacaaa gagctcgctg agctttgaat atgtgttcat
     7141 gagggtgtca agctctgcag gattttcatc ttctgcaaaa ctgccgattt gatgggacag
     7201 gtcatggagt ttttcctcta ttttgataac ctcttcaaaa ataaggagca tttcatccat
     7261 aatgctcata gggctttcaa taattgtatt ttgcctcaaa tagcctaatt ttttgccttt
     7321 tgccaggaat atttcacctg aatcaggctc cagattccct gtgagtatat taaacaaggt
     7381 tgttttgcct gctccgttaa ggcctattaa cccaaccttt tcattatcgt ttatagtaaa
     7441 tgaaatatct tcaattatat tttccaccac ataggccttt gaaacattgt tgcatgatag
     7501 aacaatcata atcgtatcct ttcaactgct cgagtatatg tttgattata ccacaactta
     7561 caatttaata aaacagaatt caaaattaat aaatttggta tcttttttta gaacgtaaaa
     7621 tatgatacaa tgtatcaatg taaggatacc cccttaaatt ttttaatttg gaggaaaaat
     7681 gggaagatta tttggcacag atggagtgcg gggaattgca aataaggatt taaaccctga
     7741 attagcattt aaattaggca ggacaggcgg atacttttta actaaaggaa taaaaagacc
     7801 aaagatggtt gtgggcatag atacgaggat ttccggagat atgcttgaag gggcactttc
     7861 ttcaggatta aattcagcag gaatagatgt tttatacttg ggaataattt caacgccagc
     7921 agtagcctgc atgattaaaa tacttgatgc cgatggggga gtgatgataa ccgcatccca
     7981 caatcccgta gaatacaacg gtataaagtt tttcaacaag gacggactaa aactgacaga
     8041 tgaaatggaa gactcaatcg aagaatacat actgaacaac atagatatag attattaccc
     8101 tgtttcaggc gacctgggaa gaaaaataag ggtagaaaac cctataagaa aatacatgga
     8161 tttcctaaaa ggaaccattg atatagatct ggaaggtctt aagattgctg ttgactgcgg
     8221 taacggggct gcttacaagt ccgcccccca gcttcttcat gagcttggag cacaagtata
     8281 cgtaatccat aatgacccga acggaattaa cattaatgta aactgcggct caacaaaacc
     8341 ggaggaaatc caaagacttg ttttggaaac aggagcagac gcaggccttt cttttgacgg
     8401 tgatgccgac agactgattg cttcggatga aaacggtaat atagtcgacg gcgaccatgt
     8461 tcttgccata tgcggcacat gcatgaagga aaaggggaaa ctgaaaaaca ataccatagt
     8521 gggaaccgtc atgaccaatt tggggcttga catatgcctt aagaaaaata atattgatat
     8581 tgtaaaaacc aatgtaggcg acagatacgt tttggaggaa atgctgaaaa acggttactc
     8641 cctaggagga gaacagtcag ggcacattat ttttcttgat tacaacacca ccggggatgg
     8701 acttttaaca gccattcagc ttttaagcat tatgaaacaa aaaaattcaa aactttcaaa
     8761 gctttcctca attatgaaga atatgcccca agtactggta aatgcccgtg tttcagatga
     8821 gaaaaagaat aattacttag aggatgaaac cataaaaaat aaaatcgcag aaatagaaaa
     8881 gcattttgat aaaaatggca gggttctgat caggtcctca ggcacagagc ctcttgtgag
     8941 agttatgatt gaaggggaag accaggaaga aataaacttc catgccgccc agctggcaaa
     9001 actgatagaa gaacgcctaa a
//
LOCUS       DAOPUT010000060         8958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_7795_length_8958_cov_20.193867, whole genome shotgun sequence.
ACCESSION   DAOPUT010000060 DAOPUT010000000
VERSION     DAOPUT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7795_length_8958_cov_20.193867"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..998
                     /locus_tag="PKK75_06025"
     CDS             150..998
                     /locus_tag="PKK75_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ83191.1"
                     /translation="MGKGKVILLPCLRNTGSENIFCTSSVAYKNEQENYYDANIKVYS
                     ELNNIISNIHGESQLIELLSKDGNISNRKYLNDGVNLIIKNCLRLTKTINNIIDVTKF
                     EKKQTQLYANNVNIVEIIDSIVINTSKQIKGKIIFDTDIEEKYISCDIRKLQKAVLIL
                     LSIAVKYSHEKEVSVYLSTVEDQININISFNNINKILFNDYLNKMDNLSPEDLDELSL
                     NFYLVKSLIYLHEGRISVGGTGDETIFKIELPCNNTDTVYYFCNNKIIDESLNQQIQI
                     EFSDIC"
     gene            1187..1762
                     /locus_tag="PKK75_06030"
     CDS             1187..1762
                     /locus_tag="PKK75_06030"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009214572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNZ83192.1"
                     /translation="MDSLKQMIIEQGEVREGNILKVDSFLNHQINPEFLYRMGEKFYE
                     LFKDKEITKILTIEVSGIAVALMAGLIFKVPVVFAKKTESLNLDKDIYSGKVFSYTKN
                     KEYNIMISKRYLDGGDKILIIDDFLAEGNAIRGLIDVAQQSGAEIKGIGIAIEKGFQQ
                     GGKLLRDEGYNLKSLVIVDELKPGSIKFRDQ"
     gene            1784..2584
                     /locus_tag="PKK75_06035"
     CDS             1784..2584
                     /locus_tag="PKK75_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNZ83193.1"
                     /translation="MPAAYAHYTFGRRVLEELNIELRENINNNIDLFHIGLHGPDILF
                     YYKPLKSNEINKLGHEIHRISADSFFERARAIISVSGDERASAYVAGFICHYMLDSQC
                     HPYIRTREDKISHNEIETEFDRAVMLKDSLNPITFKPTGHIVPSIENARCISRFFPEA
                     APEEILKSLGSMKFYLNLLVAPGKVQRALLTTALKITGSNDKIDLIKKYSPNEECLEI
                     NDELMRLYNEGIKPAARLIEEYFNSLPPGGKEIFPGAKLNERFNRNFG"
     gene            2721..3992
                     /locus_tag="PKK75_06040"
     CDS             2721..3992
                     /locus_tag="PKK75_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ83194.1"
                     /translation="MNKSEGKLTRLEKSWILYDVGNSAFIMLISTIIPIYFKNVASAD
                     GVTSANSTAYWGYAMSISTLIVAAIGPVLGALADTRGYKKPMFTLFLMLGALGCASLS
                     LPVPWIIFLAVFVFARVGISGSFIFYDAMLPDVTSDERMDLLSSHGFAWGYIGSCIPF
                     TISLLMILSAEKIGITTAVATGAAFILNALWWVLFTLPLLKNYKQLHYVEVDENPVAE
                     AFKRLGRTFKDIKKHKEIFVFLLAFFFYIDGVDTIIGMATSYGKDVGISDNNLLLALL
                     LTQVVAFPCAIAFGKLSKIYKTTDLIGVCIIGYFLITMFALQLDKAWEFWFLAVCVAV
                     FQGAIQALSRSYFAKIIPKESSSEYFGFYDIFSKGAAFTGTTLMGITTQLTGNSKFGV
                     LMISFMFIIGYVIFKKATRMNISMGTHEQTK"
     gene            4036..5421
                     /gene="amrA"
                     /locus_tag="PKK75_06045"
     CDS             4036..5421
                     /gene="amrA"
                     /locus_tag="PKK75_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNZ83195.1"
                     /translation="MAIVNSFIVPHPPLIIPEIGRGEERKIQKTIDAYHNIGKQISEI
                     KPDTIIITTPHSIMYSDYIHISPGDEARGSFREFGHSNVSMEAEYDLELVKEISIEAE
                     KEGISAGTLGEKSSSLDHGVMVPLYFVNKYYSDYKLVRISISGLPYLDHYKFGKCIKK
                     AAEKSNKKIVFIGSGDLSHMLKDAGPYGYREEGPVFDREVTRAMKTGNFLEFLKFDGD
                     FCESAAECGLRSFIIMAGATDGKKVSPELLSYEGPFGVGYAVAAFKVLGDDEGRRFEE
                     IYLNSEMERIESLKREEDEYVRLARQTLESYVINRKKIKKPDNLSSELLKTKAGVFVS
                     LKLDGSLRGCIGTTSSTTPSIADEIIQNAVSAATEDPRFPPVREDELKRLVYSVDVLG
                     EAEKIKSIDELNPEIYGVIVSKGHRRGLLLPNLEGIDTAEKQVSIALRKAGIYPEEKY
                     QLERFQVVRHK"
     gene            5418..6248
                     /gene="amrS"
                     /locus_tag="PKK75_06050"
     CDS             5418..6248
                     /gene="amrS"
                     /locus_tag="PKK75_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNZ83196.1"
                     /translation="MSSVICNICPHHCNLKEGQTGLCRARKNINGQITAINYGKLTAL
                     ALDPIEKKPLNRFYPGSSILSVGSFGCNLKCSFCQNHHISMASENEIKVQSVKPEELI
                     NIGLDLKNRGNIGIAYTYNEPLVGYEFVRDCAKLARDNNLKNVVVTNGCFCREPMKEL
                     LPFIDALNIDLKGFTNGFYKKLGGDLDTVKNFIKLAAKHCHVEVTTLIIPGENDSEDE
                     MREISEFILSVSSDIPLHISKFFPCYKMKDKGPTEVKKVYKLADIAREKLKYVYEGNV
                     "
     gene            6248..7063
                     /locus_tag="PKK75_06055"
     CDS             6248..7063
                     /locus_tag="PKK75_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNZ83197.1"
                     /translation="MNFFSGFLKGLALGAAAIAPGVSGGALALIFGLYDRITYFIAHF
                     TRNFKENILYFMPVGIGGAAGVLAFSRIIEYLFHYHEIEVKYAFIGLMLGTMPLVVSQ
                     ANKKGYKQTYLIPFIITLTITLLGAYLESNAVNVIMGAGTSPFHLIIYGIIIGFGTII
                     PGVSSSIILMYTGAYEIVIGAVSNIQIPLLFYMGIGFVLSVLLFARIISMLFEKAYGI
                     TYYAILGLVAGSVISIFPGFHMNLDYLLNLIILAASCWLSYSLSKFGRRNSLS"
     gene            7336..8793
                     /locus_tag="PKK75_06060"
     CDS             7336..8793
                     /locus_tag="PKK75_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNZ83198.1"
                     /translation="MVTFLIGMLILLGGGFLYGSYCEKVFGPDDRETPAVTKADGVDF
                     VVMKKWKNSLINLLNIAGTGPVLGPIQGILFGPVAFITIPLGCVLAGSMHDYFSGMIS
                     MRNGGAQMPKLIKKFLGGKVFGVYNVVIWILMLLVGVVFVYTPGDLIVSELLGQKAVA
                     DNPTTWIVYAGIFLYYIVATIFPIDAIIGRVYPIFGAFLIISAVGIFFGIMFGGGAYL
                     QNLSFTGMFSQHPGKLPFIPVFFITVACGILSGFHATQNTLIARTVSSEKEGKMTFYN
                     MMLVEGFIAMCWAGGAMVLFGKGTDMATGATLMVGLISRDFLGNFGALFAIIGVIVLP
                     ITSGDTAFRGLRLMVSEQFNIDQTSTQKRVSLSIAFFIPAAVILYIAKTNPAGFSILW
                     RYFAFTNQFVATFALATISIYLYINGKNYLVSLIPGTFYFFVTLSFIFNAKIGFNLPW
                     SVAYILAAVCCAAYIWFVRQRATTQKDKIIQNVLS"
BASE COUNT         2913 a         1511 c         1877 g         2657 t
ORIGIN      
        1 ggtgtcaaca aataattttc acattttgca aatttcgaca taatcttttt ataaaagctt
       61 tataataact tttgtgagga ttttttctgt tttatctatt tttctgacaa aatccaacac
      121 actacatata ctctaaaggg gtggttaaat tgggaaaagg caaggttatt ttgctgcctt
      181 gtttaagaaa cacaggcagt gaaaatattt tttgcacatc aagtgtggca tacaagaatg
      241 aacaggaaaa ctattacgat gcaaatatta aagtatacag tgaattaaac aatattatca
      301 gtaatattca cggagaaagc cagcttattg agcttctttc aaaagacgga aatatctcaa
      361 acaggaaata tttgaatgat ggagtcaatt tgataattaa gaattgtttg agactgacaa
      421 agacaataaa caatataatt gatgtgacta aatttgaaaa gaaacaaact cagctttatg
      481 caaacaatgt aaatattgtg gaaattatcg acagcattgt aattaataca tctaagcaaa
      541 tcaaaggtaa aattattttt gacacggata tagaagagaa gtatatatca tgtgatatca
      601 gaaagcttca aaaagccgtt ctgattctct tatctattgc agtcaaatat tcccatgaaa
      661 aggaagtatc agtatattta agcaccgttg aagatcagat caatattaat atttcattta
      721 ataatataaa caagatatta ttcaatgact atttgaataa aatggacaac ctttcgcctg
      781 aagacttgga tgaattatct ttaaattttt acttggtaaa atctttaatt tacctgcatg
      841 aaggcaggat ttcggttgga ggcaccgggg atgaaacaat ttttaaaata gagcttccat
      901 gcaataatac agatacagtt tattatttct gcaataataa aattattgat gaatcactaa
      961 accagcaaat acagatagaa ttttcagata tatgttaaac ggtgggacag cgcagcttaa
     1021 taatacaaat attttatggc gtctcgcttg cttttgcttt cattggcggt gtcagacttc
     1081 taaacagagt caggtcatct tttattgtca ctcaggacta tagaatgaga ttgatttttt
     1141 tcggattatc tgatatatta ttaaaaaaaa caaagaggca ggggtcatgg acagtttaaa
     1201 gcaaatgatt atcgagcagg gcgaagtaag ggaagggaac attcttaagg ttgacagctt
     1261 tttaaatcat cagattaatc ctgaattttt ataccggatg ggggagaaat tctatgagct
     1321 tttcaaggat aaggaaataa caaaaatttt aaccattgaa gtaagcggta tagcagttgc
     1381 attaatggca ggcttaatat ttaaagtgcc tgttgttttt gccaaaaaaa cagaaagcct
     1441 gaatttggat aaagacattt attccggcaa ggttttttca tataccaaaa acaaagaata
     1501 caatattatg ataagcaaaa gatatcttga cgggggtgac aagattctca taattgatga
     1561 tttcctggct gaaggtaatg caatcagggg cttaattgac gtggcgcagc aaagcggcgc
     1621 agaaataaag ggtataggca ttgcaattga aaagggattt cagcagggag ggaagctcct
     1681 aagagacgaa ggatacaacc ttaagagtct ggtaatagta gatgaattaa aaccaggcag
     1741 tataaaattc agagaccaat aaactcagca aggagggtga aatatgcctg cagcttatgc
     1801 tcattacaca ttcggcagga gagtcctgga agagttaaat atagaactga gggaaaatat
     1861 caataataat attgatttat tccacatagg cttgcacgga ccggatatac ttttttatta
     1921 taagccctta aaatcaaatg aaattaataa attaggacat gaaatccaca ggataagtgc
     1981 cgatagtttt tttgaaagag ccagggcaat aatatcagta tcaggtgatg aaagagcttc
     2041 agcatatgtt gcaggattca tatgccatta tatgctggac agccagtgcc atccatacat
     2101 aaggacaagg gaagacaaga tttcccacaa tgaaattgaa accgaatttg acagggcagt
     2161 tatgcttaag gatagtctaa atccaattac gttcaaacca acggggcaca ttgtaccaag
     2221 cattgaaaat gcaaggtgta tttccaggtt tttcccggag gctgcacctg aagaaatact
     2281 caagtccctg ggatcaatga agttttattt aaacctgctg gttgcaccgg gcaaggtcca
     2341 aagagccttg ttaacaacag cgctgaaaat tacaggaagt aatgataaga tagacctcat
     2401 aaaaaagtat tctcccaatg aagaatgcct tgaaataaat gatgagctta tgagattata
     2461 caatgaagga ataaaacctg cagcaaggtt gattgaagag tatttcaaca gtttgccccc
     2521 gggcggaaaa gaaatttttc cgggtgccaa actcaatgaa agatttaata gaaatttcgg
     2581 ataaactgct agaaagcagt ttttcttttg cttgtaaaat cataaattat ataataatat
     2641 atagggggat accggttgtt tcgtgtttag ccgctgtgaa atgaggatac cctaaaattt
     2701 gctattataa ggtggagatt atgaataaat ctgaaggaaa acttaccagg ctggagaaat
     2761 cctggatttt gtatgatgtt ggaaactcgg catttataat gcttatatca acaatcatac
     2821 ctatttattt taaaaatgtt gcttcagcgg acggtgttac atctgccaat tcaactgcct
     2881 actggggata tgccatgtcg atttcaacac ttatagtggc cgcaataggc cctgtattag
     2941 gagccttggc agacacaagg ggatataaga aacccatgtt tacacttttt ttaatgcttg
     3001 gagcccttgg gtgcgcatca ctttcactgc ctgtgccctg gataattttc cttgctgtgt
     3061 ttgtatttgc tagggttggt atttccggca gctttatatt ttacgatgca atgcttccag
     3121 acgtaacatc agatgaaaga atggatttat tatcttccca cggctttgcg tggggttata
     3181 taggaagctg cattcctttt acaatcagtc tcttaatgat attgtctgca gaaaaaatag
     3241 ggattaccac agccgtggca acaggagctg cttttatact aaatgcctta tggtgggttt
     3301 tattcaccct tccgcttctt aaaaactaca aacagcttca ttatgtggaa gtggatgaaa
     3361 atccggtggc tgaagcgttt aagcgtcttg gaagaacatt taaggatatt aaaaaacaca
     3421 aggaaatatt tgtttttttg ctggcatttt tcttttatat agatggtgta gatacaatta
     3481 taggtatggc tacatcatat ggcaaggatg tggggataag cgacaataat cttcttttag
     3541 ccctcctctt gactcaggtg gttgcttttc cttgtgcaat agcctttggt aagttgtcta
     3601 agatatataa aaccacagat cttataggtg tttgtataat tggttatttc ttaataacca
     3661 tgtttgcgct ccagcttgac aaagcctggg agttctggtt tttggcagtc tgtgtagctg
     3721 ttttccaggg agccatacaa gctctttccc gctcctactt tgcaaagata attccgaagg
     3781 aaagctccag cgaatatttt gggttctatg acattttcag caaaggtgca gcctttacgg
     3841 gaacaacact tatgggaatt accacccagc taaccgggaa ttcaaaattc ggcgtattga
     3901 tgattagttt tatgtttata ataggatatg taatatttaa aaaagcaaca aggatgaata
     3961 taagtatggg gacacacgaa cagacgaagt aaggctcacc tttataatgt ccccgcattt
     4021 agaaaggaga aaaccatggc cattgtgaac agttttatag tacctcatcc tcccttgata
     4081 attccggaaa ttggtagagg agaggaaagg aaaattcaaa agaccataga tgcatatcac
     4141 aatattggaa agcaaatttc tgaaattaaa cctgacacaa taattataac aaccccccat
     4201 tcaataatgt attcagacta catacatatt tcaccggggg acgaagcaag gggcagcttc
     4261 agggagttcg gccacagcaa tgtttccatg gaagcagaat atgaccttga gctggttaaa
     4321 gaaatttcca tagaagcgga aaaagaaggg atttccgccg gaaccttagg cgagaaaagc
     4381 agctcattgg accacggggt tatggtgcct ctttactttg tgaacaaata ctacagtgat
     4441 tataagcttg tgcgcatatc catatcaggg ctgccttatt tggaccatta taaattcggt
     4501 aaatgcatta agaaggcagc agaaaaaagc aataaaaaaa ttgtatttat tggaagcggg
     4561 gacttatccc acatgctgaa ggatgcggga ccctacggtt acagagagga agggcctgta
     4621 tttgacagag aagtaaccag agcaatgaag acagggaatt ttttagagtt tttgaaattt
     4681 gacggggact tctgtgaatc tgctgctgaa tgcggtcttc ggtcttttat aataatggct
     4741 ggggcaacgg acgggaaaaa agtaagcccg gaactcttgt cctatgaagg accctttgga
     4801 gtaggctatg cagttgcagc atttaaagtt ttaggagatg atgaaggacg ccggtttgaa
     4861 gaaatatatt taaacagcga aatggaaaga attgaatcat taaagaggga agaggacgaa
     4921 tatgtaaggc ttgcccgcca aacgctggaa agctatgtga ttaacagaaa aaagataaag
     4981 aagcctgata atctgagctc tgaactattg aaaaccaagg ccggagtgtt tgtatccctg
     5041 aaactggacg gcagtctcag gggctgcata ggaactactt catccacaac tccaagcatt
     5101 gccgatgaaa ttattcaaaa tgcagttagt gccgcaaccg aggaccccag gtttcctcct
     5161 gtacgagaag atgagctgaa aagactggtg tacagtgttg atgtgcttgg tgaggcagaa
     5221 aaaataaagt ccatagatga acttaatcct gaaatctacg gcgtcatagt ttcaaagggg
     5281 cacaggagag gactgcttct gcctaatctg gagggaattg acacagcaga aaaacaagta
     5341 tctatagctt taagaaaggc tggaatttat ccggaagaga aataccagct ggaaagattc
     5401 caggtggtaa ggcataaatg agcagtgtaa tttgcaatat atgcccccat cattgcaatt
     5461 taaaagaagg tcagacagga ttatgcagag cgagaaaaaa cataaatggc caaatcactg
     5521 ctattaatta cggtaaactg acggctcttg ctcttgaccc tatagaaaaa aaacccttga
     5581 acaggtttta tcccggaagc agcatattgt cggttggaag tttcggctgc aacttaaaat
     5641 gttccttttg ccagaatcat catatatcca tggcatcaga aaatgaaatc aaagttcagt
     5701 ctgtaaagcc ggaagaattg ataaatatag gattggactt gaaaaatagg gggaatatag
     5761 gtatagcata cacctacaac gagcccctgg taggatatga atttgtaaga gactgtgcca
     5821 aactggcaag ggataataat ttgaaaaatg tggttgtaac aaacggatgt ttttgccggg
     5881 aacccatgaa ggaattactg ccctttattg atgccctgaa cattgactta aaagggttca
     5941 ccaatggatt ctacaagaag ctgggtggtg atttggatac tgttaaaaac tttattaaac
     6001 ttgctgccaa gcactgccat gtggaagtga caacactcat aatccccgga gagaatgact
     6061 cggaagatga aatgagggag atttctgaat tcatattatc tgtaagctct gatatacccc
     6121 tgcatatatc aaaatttttc ccctgctaca aaatgaagga taaaggtcct acagaagtta
     6181 aaaaggttta caagcttgca gatatagcaa gagaaaaact taaatacgtc tatgaaggaa
     6241 atgtgtaatg aatttttttt caggattttt aaaaggtttg gcactgggtg cggcagctat
     6301 agcaccggga gtaagcggag gagcattggc tctaatattt ggcttatatg accggattac
     6361 atattttatt gctcacttca ccagaaattt taaggagaat atactttatt tcatgcctgt
     6421 gggtatcgga ggggctgcag gagttttggc cttcagcaga attatcgaat acctctttca
     6481 ttatcatgag attgaagtaa aatacgcctt cataggactg atgctgggca ccatgcccct
     6541 ggtagtcagt caggctaata agaaaggtta taaacaaaca tatttaatac cttttatcat
     6601 aaccctgacc ataactttac tcggtgcata tttagaaagc aatgcagtta atgtaataat
     6661 gggtgcaggt acttctcctt ttcatttgat tatatatggc ataataatcg gattcggaac
     6721 aattattcca ggtgtcagct catctataat acttatgtat acgggtgctt atgaaatagt
     6781 cataggtgct gtatcaaaca ttcaaattcc tttacttttt tatatgggca taggctttgt
     6841 gctgagtgtt ctcctgtttg caagaataat aagtatgctt tttgaaaagg catacggaat
     6901 tacatactat gcaatcttag gtctggttgc agggtccgtt atttccatat tccccggctt
     6961 tcatatgaac ttggattatt tattaaacct gataatttta gctgcttcct gctggttatc
     7021 gtattcattg tctaaatttg gaagaaggaa ttccttatcc tgatatttta aatatgcttt
     7081 gctgtcccga gggatatttt ttttgtctga gaaaagtgtc attataaata ttattacatt
     7141 aaattattat aaaaaatttt catcttattg aaaaataaaa caatattcgg aatactactt
     7201 gacagttttc tgtgcaaatg ttaaaatcta atagcattaa aaggaggcga aaattaagtt
     7261 tcaaaaggtt tcaaagaagt acaagtcttc attcatttta attttttaat tttattattt
     7321 ttataggagg tttttatggt tactttttta attggtatgc tgattctttt aggcggcgga
     7381 tttctttacg gctcttactg cgaaaaagtt ttcgggcctg atgacaggga aacaccggct
     7441 gttactaaag ccgacggtgt agactttgtt gtaatgaaaa aatggaagaa ctcattaatt
     7501 aacttactaa acattgcagg tacaggacct gtactagggc ctatacaagg tatcttgttc
     7561 ggacccgtgg catttataac cataccattg ggatgcgtac ttgcaggctc aatgcatgat
     7621 tatttttcag gcatgatttc catgcgtaac ggcggagctc aaatgcctaa attgattaag
     7681 aagtttttag gcggtaaagt attcggcgta tacaatgttg ttatctggat actgatgctt
     7741 ttggttggtg ttgttttcgt ttatacacct ggagacttga ttgtatctga attattagga
     7801 cagaaggcgg ttgctgataa cccgacaaca tggattgttt atgcaggaat tttcttatac
     7861 tacattgttg caacaatatt ccctatcgat gctataatag gaagagttta tcctatattc
     7921 ggcgccttcc tgattatatc agctgttggt attttcttcg gaataatgtt cggaggagga
     7981 gcatatttac agaacttatc attcacaggc atgttcagcc agcatcctgg aaaactgcca
     8041 tttattcctg tattcttcat aacagttgca tgcggtatcc tgtcaggctt ccatgcaact
     8101 cagaacacct tgattgccag aaccgtttca agcgaaaaag aagggaaaat gacattctac
     8161 aacatgatgc ttgttgaagg cttcatagca atgtgctggg caggcggtgc aatggtattg
     8221 ttcggtaaag gaacagacat ggctaccggt gctacattga tggtaggact tatatcaaga
     8281 gacttcttag gaaactttgg agcactcttt gcaataatcg gtgttatagt tcttcctata
     8341 acatcaggag acacggcatt cagaggactg agactgatgg tatcagagca gtttaacata
     8401 gaccagacct ctactcagaa gagagtaagc ttaagtattg cattcttcat ccctgcagca
     8461 gtaattctat atattgccaa aacaaatcct gcaggattct caatcctttg gagatacttt
     8521 gcatttacaa accagtttgt tgcaacattt gcactggcaa caatatcgat ttatctctat
     8581 ataaacggca agaattacct tgtatcattg atacccggaa cattctactt cttcgttaca
     8641 ttaagcttca tatttaacgc aaaaattggt ttcaacctgc cctggtctgt ggcatatata
     8701 ctggcagcag tatgctgtgc agcttatata tggtttgtaa gacaaagagc aacaactcaa
     8761 aaagacaaaa ttattcaaaa tgtactatca taaatatgcc ctaaccctat tttttcaagc
     8821 caaagcgcag aaaaaatagc ggtaggtcat tcgcaacgag cgccaatttt atgcgaccag
     8881 agggagcaaa taaaattgtg cagcgagact gcaagatgcc ctaacaaatc aggctgcttc
     8941 ggcagccttt ttttaatc
//
LOCUS       DAOPUT010000061         8859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_7918_length_8859_cov_18.206383, whole genome shotgun sequence.
ACCESSION   DAOPUT010000061 DAOPUT010000000
VERSION     DAOPUT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7918_length_8859_cov_18.206383"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKK75_06065"
     CDS             complement(<1..443)
                     /locus_tag="PKK75_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNZ83199.1"
                     /translation="MFLLTYSYNNQVSAGFLNEEKTGIIPASKVLQGAENLNMIGIIE
                     MFESINLEELKNSIDTYKEAVKLSEVKVLPPIPYPRRNVICLGKNYLDHINEVKSLKN
                     VNSDIPQKPIYFSKIAYPATGHGDVVDSHSHVVGQLDYEAELAVI"
     gene            619..>1590
                     /locus_tag="PKK75_06070"
     CDS             619..>1590
                     /locus_tag="PKK75_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HNZ83200.1"
                     /translation="MNRSVLNELHKEIIFDLTKKEFLKSIDISEEKMQSYLINAKFTG
                     DLSTFIDRKTVTCKNVLDLSKNIIFYGHPAPPEDWLAYIFQYVLNKSFPEAVTIDLNP
                     EYEVSAVIYLQMLRTVIKYAQKNNWGRIPKFEFLTEKEVNDLQNSQEYLSFLDAFDKN
                     FIYELMMLDGEVNGYNTLNHVSSVHHVAMHVARQIKKTGIQVNLGLVSGSAAGHDIGK
                     YGCKGLEKRRVAYLHYFYTDQWFNKYNMPGIALIAANHSTWDLELENLSLESLLLIYA
                     DFRVKNKKIETGEEMHIFSLTESFGIILNKLDNVDEAKEKRYIRVYSK"
     assembly_gap    1591..1690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1691..>3897
                     /locus_tag="PKK75_06075"
     CDS             <1691..>3897
                     /locus_tag="PKK75_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HNZ83201.1"
                     /translation="EENAGLSLWDKYLGLFLDPGYKVTDKQKEWIGYSLRIFVDSVIN
                     SKRNKSQKDYVEIYLKHLHGDINDDTAKFNGLNSLLSIPMDLYNKDQLAFVLELSLKL
                     VKESSYSIRLMAAQFIYKAVNQVKITGHSLSDVLAILEEFSPDEGLCMNYLKYKTARS
                     LNAPASLLKKYSNLLSGNWYKTSDIFLNNLKAATPWNVKTISIDYIMENLSQRNEVAL
                     LQAATHLANLVKVSAMESVRNKAGNSLVQLGPMLTIDQRNEIAFELIKGLEIDEMQYA
                     KYIPEYLGRFVMLLSPKELDEFIIDLKNIYNNSSERSSALVIHTFGIIVQHYPEYKER
                     FGEDNSVLDKRLIKILGIILGGLANFNSQVKQETFLVIGQYIFGSQILTLSQKNKIFS
                     LIYKKLLTLISEKELSELFFFNNSASFNHIYRFISDYEFFNGKFNIKENKNIAFFPGT
                     FDPFSLSHKGIVKEIRNLGYDVYLAVDEFSWSKKVQPRMIRRQIINMSIADELGVFLF
                     SDDIPVNLSNNDDLDVLRSLFPKKDVYMVVGSDVIINATAYKNKTEENSIHSFNHIVF
                     KRAKDDLTKDAAKKAEEAKERIKGTLVELQLPLYLEDISSTQIRENIDNNRDISNLID
                     TMAQNFIYDRNLYIREPLNKAVLKTKPFDIEIVEEFSRKTLDEIGFCIFNNGDFYENM
                     GERLASKNVKLLIIRDSKSNKMLGFSAFHKISTSDVYSEFKSSYIANYVREKTS"
     assembly_gap    3898..3997
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3998..4974
                     /locus_tag="PKK75_06080"
     CDS             <3998..4974
                     /locus_tag="PKK75_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HNZ83202.1"
                     /translation="NLESFIKDPLNDNKKVHEAISFTRKKLQKSMTDLYPGSLVLSID
                     NDMINQILIKKICSINNVPNEPQVPRVLGENMVVPFGNVLKGMVVPNTVTKSLHTEKV
                     YSSDTKKFKIVEYPFYSPIENQVRTIESFQKPVILVDDLLHKGYRIKEIDPILKRHNI
                     NVKKIIVGIMSGRGKDLMDIQGREADYAYYIPNLRLWFNENLMYPFLGGDGIWIENEN
                     ITNLIPSINLVLPFYSPMFIRGASKESIYNLSMVCLENAKQVLLALEKEFKEIFERNL
                     TVKRLGEVLLSPRLPYIGDNIYYDLNKEASDFMDVSIESLSKLERIIK"
     gene            4974..6167
                     /locus_tag="PKK75_06085"
     CDS             4974..6167
                     /locus_tag="PKK75_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HNZ83203.1"
                     /translation="MFYYKYKDKYLFSVKDEYNYEKTNIIPDDCRELYFLNSLINDAK
                     KSFAINHPSDLFIEEESLDMLRADDKSYDLPEIIINLIDKRKIKAVNTAYNDYENCLQ
                     MDEPGKRKVNILALGDVGSTLLIGLRLLGKDAVSSIGIYDRSRDKIKRWEYEMNQTSY
                     PFEDSLPEVGGIEKEELFDCHMFLFCASKGVPPVGSEAADVRMVQYEENAKLIKEYAS
                     RAADCGFKGIFAVVSDPVDLLCQTVLKESRGVLAPEQIKGYGLGVMNARALYYSKKFK
                     RFSMYLKEGRAFGPHGKDLVIANSINDYDDELSRELTKFAVEANLEVRKTGFKPYIAP
                     ALSSGAISIVYTLEGKYHYSSNYIAGVYMGAKNRNLPSGLDIEKIKMPDKLFERIKET
                     YGRLR"
     assembly_gap    6181..6280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6281..6755
                     /locus_tag="PKK75_06090"
     CDS             <6281..6755
                     /locus_tag="PKK75_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNZ83204.1"
                     /translation="INEIHIENGSVVDCKGCSYKACKHYSQSTNCFYGGIMVEEIYPA
                     ILDCDVLIMLCPNYNDSINANMSAVINRLTALYRKTKFYDKYFYSIIVSGFSGSDIVA
                     RQLISAMNINKTFMLPQNFALMETANNPGDIEKIENIREKAKEFAENINSFVEII"
     gene            complement(6801..6874)
                     /locus_tag="PKK75_06095"
     tRNA            complement(6801..6874)
                     /locus_tag="PKK75_06095"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6838..6840),aa:Pro,seq:ggg)
     gene            7017..7439
                     /locus_tag="PKK75_06100"
     CDS             7017..7439
                     /locus_tag="PKK75_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HNZ83205.1"
                     /translation="MNDKELFGCGCKDEDCGCNHDEFDDNCECGCGCGDHEEEVMTIT
                     LEDGTEVDCAIIAIFPVEEKDYIALLPLDGNEEEGEVFLYEFKEQGDGIELLSIESDK
                     EYELVTNAFDEILEEADLEELFDEDEEEDEYEDDDDED"
     gene            7511..>8859
                     /locus_tag="PKK75_06105"
     CDS             7511..>8859
                     /locus_tag="PKK75_06105"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNZ83206.1"
                     /translation="MAFIINEPSRTFGEYLLLPNLTTKDCIVDNVDLSTPITRFKKGG
                     TPEIKLNIPFSSAIMQSVSDNNMAIALAKNGGISFIYCSQPIAVEAEMVKKVKKYKAG
                     FVISDSNLTPEHTLADVLDLKAETGHSTMAITEDGSATGRLLGIVTGRDYRTSRDSLD
                     KKVKDFMTQGENLITGKEGISLSEANDIIWEHKLNSLPIVDKNFNLVYMVFRKDYSED
                     KDNPNSLLDEHKRYVVGAGINSRDYKERVPALVEAGADILCIDSSEGFSQWQHDTITW
                     IKKTYDGRIKVGAGNVVEKEGFKYLADAGADFVKIGIGGGSICITREQKGIGRGQASA
                     VMDVAKARDEYFEETGDYVPICSDGGIVLDYHITLALAMGADFVMLGRYFAGFDESPT
                     NKIKYGSGYVKEYWGEGSNRARNWARYDLGGKGGFQFEEGVDSYVPYAGKLDDGLGST
                     "
BASE COUNT         2928 a         1458 c         1790 g         2383 t
ORIGIN      
        1 attattactg ctaattctgc ttcatagtcc aattgtccga caacatgtga gtgtgaatca
       61 acaacatctc catgccctgt ggcaggatat gcaattttac tgaagtaaat aggtttttgc
      121 ggtatatctg aattcacgtt tttcaaactc ttaacctcat taatgtggtc aagatagttt
      181 ttccccagac aaattacatt tcttctggga tatgggatag gcggcaaaac tttgacttcg
      241 ctgagcttga ctgcttcttt gtatgtatca attgaatttt ttaattcttc taaattaata
      301 ctttcaaaca tttcaataat gccgatcata tttaaatttt ctgcaccttg caaaactttc
      361 gacgcaggaa ttattccggt tttttcttcg tttagaaagc cggcggacac ctggttatta
      421 taggaataag tcaataaaaa cataaaaacc tccaaagctt tgatatatag actatattat
      481 attcaaaaac ttttttcaag acaaagttaa aggcattaca gtaccttcat ccctctgcca
      541 tcccattatc attaaaatat tgcggtatgg aacttgatat ggtataattt tgaaaatgaa
      601 atatgcaggg aggctgccat gaacaggtct gtattaaatg aattgcacaa ggaaatcata
      661 tttgacctta ctaaaaaaga gtttttaaaa tccatagata tttctgagga gaaaatgcag
      721 tcttacctga ttaatgcaaa attcacggga gacttatcaa cttttattga tagaaaaaca
      781 gtaacatgca aaaatgtact tgacctgtct aaaaatatta tcttttacgg gcatccggcc
      841 ccccctgaag actggcttgc ctatatattt caatatgttt tgaacaaatc ctttccagaa
      901 gcagttacaa tagaccttaa ccccgaatat gaagtttcag ctgtcatata tcttcagatg
      961 ctgagaactg ttattaaata tgcacagaaa aataactggg gcaggattcc aaaattcgaa
     1021 tttctaactg aaaaagaggt aaatgacctt cagaacagtc aggaatactt aagctttctg
     1081 gatgcatttg acaagaattt tatttatgag cttatgatgc tcgacgggga agttaacggc
     1141 tacaacacat taaatcatgt ttcctcggta catcatgttg ctatgcatgt tgcaaggcag
     1201 ataaaaaaga caggaataca ggtgaacctg gggcttgttt ccggttccgc agcaggtcat
     1261 gatattggaa agtacggatg caagggcctg gaaaaaagaa gggttgcata tcttcattat
     1321 ttttatacag accagtggtt taacaagtat aatatgcctg gaattgccct tattgcagca
     1381 aatcattcaa cctgggacct ggaacttgaa aacttatccc tggaatcctt gcttttgata
     1441 tatgccgatt tcagggtaaa aaataaaaag atagagaccg gggaagaaat gcacattttt
     1501 tctttgacgg agtctttcgg tattattctt aacaagcttg ataacgtaga tgaagcaaag
     1561 gaaaaaagat atataagggt atattcaaag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn gaagaaaatg ccggtttatc cctgtgggat aagtacctgg gacttttcct
     1741 cgacccggga tataaggtta cggacaaaca aaaagaatgg ataggatatt ccttaagaat
     1801 atttgttgac tcggtaataa attccaaaag aaacaagtct caaaaggatt atgttgaaat
     1861 atacctgaaa catctccatg gggacataaa tgacgataca gctaaattca acggtttaaa
     1921 ctccctatta agcatcccca tggacttgta caacaaggac cagcttgcat ttgtgcttga
     1981 gctttcattg aaacttgtca aagagtcttc atacagcata agactgatgg cagcccaatt
     2041 tatatacaag gctgtgaacc aggtaaaaat cacagggcac tccctcagtg atgtgcttgc
     2101 aatattagag gagttttcac ccgatgaggg tctgtgcatg aattatttga aatataagac
     2161 agcccggtct ttgaatgctc ctgcttctct tcttaaaaaa tactcaaatc ttttatcagg
     2221 gaactggtat aaaacatcgg atatattctt aaataattta aaggctgcaa ccccctggaa
     2281 tgtaaaaacc ataagcatag actacataat ggaaaatctt tctcaaagaa atgaggttgc
     2341 cctcctacag gcggcaaccc atcttgccaa tcttgtaaag gtaagcgcca tggaatccgt
     2401 aagaaacaag gcaggtaact ccctagtaca gctgggtccc atgcttacca tagatcagag
     2461 aaatgaaata gcatttgagc ttattaaagg tctcgaaata gatgaaatgc agtatgcaaa
     2521 gtatattccc gagtatttgg gacgctttgt tatgctctta tctccaaaag agctggatga
     2581 gtttattatt gacttgaaaa atatttacaa taactccagc gagcggtcca gtgccttggt
     2641 tatacacaca ttcggaataa tagtccagca ctacccggaa tacaaggaaa gattcgggga
     2701 agacaacagt gttttagata aaagacttat aaaaattctg ggaataattt taggaggtct
     2761 agccaatttt aattcccagg tgaagcagga aacatttttg gttataggcc agtatatttt
     2821 cggttcacaa atactgactc taagccagaa gaacaagata ttttctctta tttacaaaaa
     2881 gcttttaaca ctaataagcg agaaggaact aagcgagctg ttctttttca acaactctgc
     2941 ctcctttaac cacatataca gatttataag cgactatgag tttttcaacg gtaaatttaa
     3001 tataaaggaa aataaaaaca tagcattttt ccccgggacc ttcgatccat tttccttaag
     3061 ccacaaggga atagttaagg aaataagaaa cctgggctat gacgtatacc tggctgttga
     3121 tgaattctca tggtccaaga aggtccagcc gagaatgata agaagacaga ttatcaatat
     3181 gtccattgct gatgaattgg gtgtgttttt gttttccgat gatataccgg taaacttatc
     3241 caataatgac gatttggatg tattgaggtc tttgtttccc aaaaaggatg tttatatggt
     3301 tgtggggagc gatgtaatta taaatgcaac ggcatataaa aacaagactg aggaaaattc
     3361 aatacactcc tttaaccaca ttgtatttaa aagagcaaag gatgatttaa caaaggatgc
     3421 tgccaaaaag gcagaagaag caaaggaaag aataaaggga acacttgtgg agcttcagct
     3481 tcctctttac ctggaggata taagctcaac tcaaataagg gaaaacatag acaataacag
     3541 agacatatca aaccttatag acactatggc ccaaaatttc atatatgaca ggaatttgta
     3601 cataagagag cccttaaaca aggcagtatt gaaaacaaaa ccttttgata ttgaaatagt
     3661 tgaagaattc agcaggaaaa ctcttgatga aataggcttc tgtattttca ataatggtga
     3721 tttctatgag aacatgggag aaaggcttgc ttctaaaaac gtcaagctgc tgataataag
     3781 ggattccaag agtaataaaa tgcttggttt ttcggcattt cataaaatca gcacttccga
     3841 tgtatattcg gagtttaaaa gttcctacat agcaaattat gtgcgggaaa aaacctcnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta atctggaaag ctttatcaag
     4021 gaccccttaa atgataacaa aaaagttcat gaagccattt cctttacaag gaagaaactt
     4081 caaaagtcaa tgaccgacct gtatccggga agccttgttt tatcaattga caatgatatg
     4141 ataaatcaaa tccttatcaa aaaaatatgc agcatcaaca atgtacccaa tgaaccccag
     4201 gttccgagag tacttggaga aaatatggtt gtgccttttg gaaatgtatt gaagggtatg
     4261 gtagttccaa atacagtaac aaaatctctc catactgaaa aggtttattc ttcggataca
     4321 aaaaaattta aaatcgtaga gtatcctttc tactcgccta tcgagaacca ggtaaggacc
     4381 atagaatcct tccaaaaacc tgtgattctt gttgatgact tacttcataa aggatacaga
     4441 atcaaagaaa ttgaccccat acttaaaagg cacaatataa atgttaaaaa aattattgtg
     4501 ggtataatgt caggcagggg caaggatttg atggacatac agggaagaga ggcagactat
     4561 gcatattata ttccaaactt aagactgtgg tttaatgaaa acctcatgta tccatttttg
     4621 ggaggagacg gcatatggat tgaaaatgaa aatattacaa atttgattcc ttcaatcaat
     4681 ctggtgcttc ccttttattc acctatgttc ataaggggag cttccaagga gtccatatac
     4741 aatctttcaa tggtatgcct tgaaaatgca aagcaggttc ttcttgcact tgaaaaggaa
     4801 tttaaagaaa ttttcgaaag aaatctcact gtaaaaaggc tgggtgaagt tcttttatca
     4861 ccgagactgc cctacattgg agacaatatt tattatgatt taaacaagga agcttcagac
     4921 ttcatggatg tgagcataga gtcgctttcc aagcttgaaa ggattataaa ataatgtttt
     4981 attataaata taaggataag tatttatttt ccgtaaaaga tgaatataat tatgaaaaaa
     5041 caaatattat tccggacgac tgccgggaat tgtactttct taacagttta atcaacgatg
     5101 caaaaaaatc ctttgctata aaccatcctt ctgatttgtt tattgaagag gaaagcctgg
     5161 atatgctcag agcagatgat aaaagctatg accttccgga aattatcatt aacctgatag
     5221 acaaaagaaa aattaaagca gtgaacacag catataatga ttatgaaaac tgtttacaaa
     5281 tggatgagcc cggtaaaaga aaagtaaata ttttagccct gggcgatgtg ggaagcacct
     5341 tattgattgg acttagactc cttggtaaag atgcagtatc atccataggc atatatgaca
     5401 gaagcaggga taaaataaag agatgggaat atgaaatgaa tcaaacatca tatccctttg
     5461 aagattccct gccggaggtt gggggcatag aaaaggaaga attgtttgac tgccatatgt
     5521 ttttattttg tgcttctaag ggagttcccc cggtaggaag cgaagcagct gatgtgagaa
     5581 tggtgcagta tgaggaaaat gcaaagctta taaaagaata tgcctccaga gcggcagact
     5641 gcgggttcaa gggaatattt gccgtggttt cagacccggt tgacctcttg tgccaaacag
     5701 ttcttaaaga aagcaggggt gttctggcac cggagcaaat aaagggatac ggccttgggg
     5761 taatgaatgc aagagctttg tattactcaa aaaaatttaa gagattttca atgtacctaa
     5821 aggaaggaag ggcattcgga cctcacggga aggatttggt aattgccaac agcataaatg
     5881 attatgatga tgagctttcc agagaactga ccaagtttgc tgtggaagca aacctggaag
     5941 taagaaagac aggattcaag ccctatattg cacctgccct gtcctcaggc gccatttcca
     6001 tagtttatac tctagagggg aaatatcact acagttcaaa ttatatagcc ggcgtgtaca
     6061 tgggggctaa aaacagaaac ctgccctcag ggcttgatat tgaaaaaata aaaatgcctg
     6121 ataaactttt tgaaagaata aaagagactt atggcagatt aagatgataa cgggaattag
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gatcaatgaa attcatatag
     6301 aaaacgggtc tgttgtagac tgcaagggat gttcctacaa ggcttgcaag cactattccc
     6361 aaagcaccaa ctgcttttac ggaggaataa tggttgagga aatttatcct gccatacttg
     6421 attgtgatgt attgattatg ctttgcccaa actacaacga ttcaataaat gcaaacatgt
     6481 cggcggtcat taaccgcttg acggcactct accgtaaaac caagttttat gataaatatt
     6541 tttattccat aattgtatcc ggattcagcg gcagcgacat tgtagcccgg cagcttataa
     6601 gcgcaatgaa tattaacaaa accttcatgc ttcctcaaaa ttttgcttta atggaaaccg
     6661 ccaacaatcc cggtgatatt gaaaaaatag aaaacattag agaaaaagct aaggaatttg
     6721 ccgaaaatat caatagtttc gttgaaataa tctgaaatat accttaaaat aaaaacaggc
     6781 ggtcaaagac cgccttgcta tcgggatgac aagattcgaa cttgcggccc ctaacacccc
     6841 atgctagtgc gctaccaaac tgcgctacat cccggcaaca tttaaattat aacggaaaaa
     6901 acagcttctg taaagaggaa atttttattt aaattaatta ttgcatttta tattccattc
     6961 tgttagagtt tatgtatcca tgtctatgga taaataaatt ataaggatgt aaaaatatga
     7021 atgataagga attattcggc tgtggctgta aagatgaaga ttgcggctgc aatcatgatg
     7081 aatttgatga taattgtgaa tgcggatgcg gatgcgggga tcatgaagag gaagtaatga
     7141 caattacact ggaagacggg acagaggttg attgtgctat tattgcaatt ttccctgttg
     7201 aggaaaagga ctatattgcc ttgcttccat tggatggtaa tgaggaagaa ggagaagtat
     7261 ttctttatga atttaaggaa cagggcgacg gaattgaact gctgagtata gaatccgaca
     7321 aggaatatga gttggtaaca aatgcatttg atgagattct cgaagaagca gatctggaag
     7381 agctgtttga tgaggatgaa gaagaagacg aatacgagga tgacgacgac gaagattaat
     7441 gtacatataa aaggaactgt tacatcaaca gttcctttgc aataagcaca ccataagaaa
     7501 aggagacaat atggcattta taataaacga gccttccaga acattcggcg aatatttgct
     7561 gttgccgaat ttaacaacta aagattgtat tgttgataat gttgatttaa gcacacctat
     7621 aacccggttt aaaaaaggcg gaacacccga aataaagctt aacattcctt tttcttcggc
     7681 cattatgcag tctgtttcgg ataataatat ggcaatagca ttggcaaaaa acggcggcat
     7741 ttcatttata tattgctcac agccaattgc agtcgaagca gaaatggtaa aaaaggtgaa
     7801 gaaatataaa gccggatttg ttataagcga ttctaatctg acacctgagc atacactggc
     7861 ggatgttttg gatttaaaag cagaaaccgg ccattcgacc atggctataa ccgaggacgg
     7921 ctcggctaca ggaaggcttc ttggaattgt aacaggcaga gattacagaa caagccggga
     7981 ttccctggat aaaaaggtta aggattttat gactcaaggc gaaaacctaa taacagggaa
     8041 agaggggata tcactttctg aagcaaatga tataatttgg gagcataagc tgaattctct
     8101 tcccatagta gataaaaatt tcaatttggt atacatggta ttcagaaaag attattcgga
     8161 ggataaggat aatcctaatt ccctcctgga tgaacataaa agatatgttg tgggagcagg
     8221 tattaattcc agagattata aggaaagagt tcccgccctt gttgaagcgg gagcggatat
     8281 tctatgtatt gactcatcag aaggcttcag ccaatggcag catgatacaa tcacctggat
     8341 aaagaaaacc tatgacggca ggattaaggt gggagccggc aatgtggtag agaaggaagg
     8401 cttcaaatac ctggcggacg caggtgccga ttttgtcaag ataggcatag gcggaggctc
     8461 aatctgcatt accagggagc agaagggaat aggaagaggc caggcatcag ctgtaatgga
     8521 tgttgctaaa gcccgtgatg aatattttga agaaaccgga gattacgtac ccatatgttc
     8581 agatggagga attgttctgg attaccacat aactcttgca ttggctatgg gagcagattt
     8641 tgtaatgctt ggaaggtatt ttgcaggttt tgatgagagc cccaccaata aaataaaata
     8701 cggaagcgga tacgttaagg aatattgggg agaaggctcc aacagggcga gaaactgggc
     8761 aaggtatgat cttggaggaa aaggcggatt tcagttcgag gaaggcgttg attcatatgt
     8821 gccttatgca ggaaaacttg atgacggact tggaagcac
//
LOCUS       DAOPUT010000062         8433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_8418_length_8433_cov_19.101456, whole genome shotgun sequence.
ACCESSION   DAOPUT010000062 DAOPUT010000000
VERSION     DAOPUT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8418_length_8433_cov_19.101456"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PKK75_06110"
     CDS             <1..1218
                     /locus_tag="PKK75_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNZ83207.1"
                     /translation="MSKYARRMKTMEEPANIIKGLFGSMNDPDVISFGGGAIAKECLP
                     VDKVREITSDIMKVEGRGYEILSYGPVLGAKDLRDVVVNDLLAPKGVKANADNIIITT
                     GGLEAMSLMCQLFIEPGDVILVESPTFVHSVETFSMFEAKCVAVEMDDDGMVTEDLEE
                     KIVKYNPKIVYVIPTFQNPTGRTLSLERRQKIAELGSRYDVIILEDDPYRDIRYSGQE
                     LPPIKAFDKTGHTVLANSFSKIFSPGSRIGYVYANDDIISKMTDAKSASNSHTSLLPQ
                     IICAEFFKRGYFPDHHKQLCDIHRERRDAMIESIDKYFPQGCKRTFPDGGLFTWVELP
                     AGINTTDLLKESAANPDIKVAFVAGEGFFTEGGGKGSNCMRLSFGANPPDKIRIGIEK
                     LGNLIKSKLVEEK"
     gene            1215..1979
                     /locus_tag="PKK75_06115"
     CDS             1215..1979
                     /locus_tag="PKK75_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNZ83208.1"
                     /translation="MIKNKLKEKLRAGETALGTFIVCNAPDLVEIAGLTGFDFVIIDN
                     EHGPMRAESSQHLIRAAEARGITPIVRIPDRLESTILHTLDIGAHGIQVPQVNDAKAA
                     MEIVEKSKYHPTGKRGLAFPRSADYGLCDISAYTGNANEETLIITHCENKTCLENLEE
                     ICKIPEIDVIFLGPYDMSQSMGVTGQVTHELVENAARKVLEITKRYNKAAGIFAGNGE
                     VARKRAEQGFKYIAIGMDTVFYSAKCAEEIRKFQIQ"
     gene            2126..3346
                     /locus_tag="PKK75_06120"
     CDS             2126..3346
                     /locus_tag="PKK75_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ83209.1"
                     /translation="MVSKRKYILTILVVVIVTVFLTLSFGQVFLAEIGQKVYLSKEEY
                     GQMREMYEKYAKQEGLMQIAKKEFLYEADEEVMLEGALQGTLKALDDPYTQFMTKEEF
                     ESLMQDTEGSYSGIGVYITASDDNKIIIVSPIEDTPAEKAGLKTGDKIIRINGVEYTA
                     DKINAAVKVMKGEPGTSVTLTIQRESADGTQNVSDVVVNREKIKMKTVKPAMLGEDIG
                     YIRITTFDMQTANDFKTAYETLKSKGIKGLVIDLRFNGGGMLNATVDITDIFTGEGIV
                     TYTKTKSGEIEYFKSDEEKEDIPIVLLINEGSASASEIMAGAMKDTKRAVLVGKKTFG
                     KGIVQKVQEFGNDGEGIKMTVSEYFTPSGINIHGIGIEPDIEVELPEDAEGYGYEFFE
                     TDTQLKKAVEVLNR"
     gene            complement(3517..5229)
                     /locus_tag="PKK75_06125"
     CDS             complement(3517..5229)
                     /locus_tag="PKK75_06125"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HNZ83210.1"
                     /translation="MKSVKTVLAVIILVLIFTLNAYGLPNEASNHYELENIMLKQMRE
                     YNTNFDIKYTGSLDNIDEVLKNMVDKDVYVKSNISRVDYNYSIPRGTAVTYINVKVSY
                     IISRDERIEADKIIDGILADIIKPNMNDHEKVKAVHDYIVLNSKYDKSEIYYSDYDLL
                     TKGTSVCNGYALLTYNMLSRLNIPVSLVYGTAGGEAHIWNMVKLGDYWFHLDVTWDDP
                     VSDRDALFYTYYMLAEKEISKDHTLDEGLKVPRSAKNYYDYLKELSYDVLLVETGLDM
                     YDEENSVKDETELNNLLTSKIKHQPLRISLRFDKSISQDSIMNAISQLANYDFISAFQ
                     YNANEIDITGKWKVLNLFIKYKETPDEILLDFARNVYNTATEVAYNVYARYGSKKVNI
                     TKDVYIYPYDRDKIDISNGTLKFKEPGNYNLLFEFQGLRETISITGLNAGAFNYITDK
                     KPDNYVNVKVYDQYIDFSSIDQWPFIENGRTMVPLRAVFEVLNCDVKWEESSKSAVVE
                     YGTTKVIIPADSTTAYINGEANSLDVPAKIVNDRIMIPLRFVSEAIKKTVIWDDPNKT
                     VLIY"
     gene            5501..5980
                     /locus_tag="PKK75_06130"
     CDS             5501..5980
                     /locus_tag="PKK75_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ83211.1"
                     /translation="MNNIIPELLKLKGFDQHTPYHDRDVLEHTMAVVDGIKPRLSLRM
                     AALLHDISKPDCFSLDEKGIGHFYGHHISSALTSEKILQRLGYEEEFIQDVTILIKHH
                     YIKYIANVITEKGIKKFVEAVGAERLDDLFELIRADMEGKASANHEVIERLRAMCNL"
     gene            complement(5957..7081)
                     /locus_tag="PKK75_06135"
     CDS             complement(5957..7081)
                     /locus_tag="PKK75_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ83212.1"
                     /translation="MKILTLCLLLLLSGCLEGPRTPDNKIPNEVPKEDKGLFYEFEEE
                     ALLLLDNMTIEEKIGQMFLARCPEEEHLKFYLSMKPGGFIMFGRDFYSKMREEVINNI
                     EYYQNNSPIPMIIGVDEEGGTVVRVSSNPNLAAKPFRSPQELFNLGGFEEIKKETEEK
                     SKLLKSLGINLNLSPVADVSTHEGDFIFDRAFGKSAEETAEYVTAVVEEAKKNKISST
                     LKHFPGYGNNADTHTGSAYDKRPYEEFLRSDFLPFKAGIDAGAESVLVSHNIVESIDS
                     ELPASLSKKVIDILRNDMDFTGIIMTDDLSMGALSEIPSPEAMAVKAGCDMLIVSDLE
                     KSYKALLTSIKNQDISEDRINESVLRIIKWKLASEIAHGS"
     gene            complement(7153..>8433)
                     /locus_tag="PKK75_06140"
     CDS             complement(7153..>8433)
                     /locus_tag="PKK75_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ83213.1"
                     /translation="LALSSVTAFADEVVIINIDSDKVEFTEVLGFPFIDENNRTQVPF
                     RAVLQKFGAEVDWDNETRTAIATKDGIVVKVPIGESYILKNDEKIDVDTASRIVNDRT
                     YLPIRAVIEAFGSDVEWDGALKTVVVTTEPVDAKALYFAANEKSYDWKNYDINAKIHM
                     SMDVPDDAGSVQPFSMDMNMYMSMFMEPFKAKITASMLVPSMEEEVLVPMVKMYMTID
                     EEGIKQYIGMADETGELEWIKQTTAMGMLSDLMKYDEETIAKNKEMIEEHTEDVKYFG
                     KYVEDGKTLLRLEYTLSGEIYKDIFAETSELMPEPATEEEKLAVEMLEGFADIELGDL
                     SYIVYIDEATGEMVKMEMDLGGMVVSMLQGMTEALGVPAEELEALKSLKALMTMEILN
                     INSAEKFEIPEEALNAPDMSQLLEQLETEPEEEL"
BASE COUNT         2681 a         1570 c         1636 g         2546 t
ORIGIN      
        1 atgtcaaaat atgcaagacg tatgaaaaca atggaggagc cggctaatat tataaaaggt
       61 ttattcggct cgatgaatga tccggatgta atttcatttg ggggtggggc aatagcaaaa
      121 gaatgcctgc cggttgacaa ggtaagggaa atcacctccg atattatgaa ggttgaggga
      181 agaggctatg aaatattgtc ctatgggccg gtgcttggcg cgaaggattt aagagatgta
      241 gtggtaaatg acctgctggc tcccaaaggg gttaaggcaa atgcagataa tattataata
      301 accaccggag gattggaagc aatgtcttta atgtgccagc ttttcattga gcccggtgat
      361 gtaattttgg ttgaatctcc gacttttgtt cattcagttg aaacattcag catgtttgaa
      421 gcaaaatgcg ttgctgttga aatggatgat gacggaatgg taacagagga cctggaggag
      481 aagattgtaa aatataatcc caaaattgtt tatgtaatcc ctacattcca aaatcccaca
      541 ggcaggacct tatccctgga gagaagacaa aaaattgctg agctcgggag cagatatgac
      601 gtaattattc tggaagatga cccctacagg gatataagat acagcggcca ggaactcccg
      661 ccaattaagg cctttgacaa aactggccac accgtactgg ccaacagttt ttcaaaaata
      721 ttttcaccgg gaagcagaat aggatatgtt tatgccaatg atgatataat ttctaaaatg
      781 acagatgcaa agtctgcttc aaattcacat acatctcttc tgcctcagat aatatgtgct
      841 gagtttttca agagagggta ttttccggac catcacaaac aactgtgtga tatccacaga
      901 gaaagaagag atgcaatgat agaaagcata gataaatact ttcctcaggg gtgcaagaga
      961 acatttcccg acggaggatt attcacatgg gtggaattgc cggcaggaat aaatacaaca
     1021 gatttattga aggagtcagc agcaaatcct gatattaagg tagcctttgt tgcaggagaa
     1081 ggatttttta ctgaaggcgg cggcaagggg agcaactgca tgcggctcag ctttggtgcc
     1141 aatcccccgg ataaaataag aattggaatt gaaaaattgg gtaatttaat caaatcaaag
     1201 ttggtggagg aaaaatgata aaaaataagc ttaaagaaaa gttacgcgca ggagagactg
     1261 cgctgggaac attcattgtg tgcaatgctc cggatttagt tgaaattgca ggtttgacag
     1321 ggttcgattt tgtaataata gataatgaac acggaccaat gagagcggaa agctcacaac
     1381 acttaataag agcagcagaa gcaaggggca taactcccat agtgaggatt cctgacaggc
     1441 tggaaagtac tatacttcat actcttgata taggagctca cggaattcag gttccccagg
     1501 tcaatgatgc aaaagcggct atggaaattg tagaaaaaag caagtaccat ccgacaggca
     1561 agagggggct ggctttccca aggtcggcag attacggctt gtgcgatata tcggcctata
     1621 ccggtaatgc aaatgaggaa accctgataa tcacccattg cgaaaataaa acatgtctgg
     1681 aaaaccttga agaaatatgt aaaatacctg aaatagatgt aatattttta ggaccatatg
     1741 acatgtccca atccatgggg gtaacggggc aggtaactca tgaattagtt gaaaatgcag
     1801 caaggaaggt ccttgaaata actaaacgat acaataaggc agcaggaatt tttgcgggaa
     1861 acggagaagt tgccaggaaa cgtgcagaac aaggttttaa atacatagcc ataggcatgg
     1921 atacagtatt ctacagtgct aaatgtgctg aagaaatcag aaaatttcag atacagtaaa
     1981 gcatattaat tttgctgcat atatgatata atttcaaata ttggttaagg ctcaattaat
     2041 tattatagga taaaataata tagctggcat ataaataaat ataagcgttt aatatcagct
     2101 taattttaat agcgggagtg agtatatggt atcgaagagg aaatacattc ttacgattct
     2161 tgtggttgtt attgttacgg tttttcttac cttgtcattt gggcaggtgt tcttggctga
     2221 aataggacaa aaggtatatt tatcaaaaga agaatatgga caaatgagag aaatgtatga
     2281 aaagtacgct aaacaagaag gcttaatgca gatagcaaaa aaagagttcc tgtatgaagc
     2341 ggatgaagaa gttatgcttg aaggagcgct gcagggaaca ctaaaggcct tggatgatcc
     2401 atatacacag tttatgacca aggaagaatt tgagtcttta atgcaggata cggaaggttc
     2461 atatagtggc atcggggttt atataactgc cagtgatgat aataaaataa taatagtatc
     2521 acccatagag gatacacctg cagaaaaggc aggattgaag acaggagata aaattatccg
     2581 aataaacggt gttgaatata ccgcagataa aattaatgcg gctgtaaaag ttatgaaagg
     2641 cgaaccgggc acaagtgtca cattgactat tcaaagggaa tcagctgacg gaactcaaaa
     2701 tgtgtcagat gtggtagtaa acagggaaaa aataaaaatg aagactgtta agcctgcaat
     2761 gcttggagag gacataggat atatcaggat tacaacattt gatatgcaga cggcaaatga
     2821 ttttaaaact gcatatgaga cactaaaaag caagggcatc aaaggactgg tcatagattt
     2881 aagatttaat gggggcggca tgcttaacgc cacagttgat ataacagata tttttacagg
     2941 ggagggcata gtgacctaca ccaagacaaa atcaggagaa atcgaatact ttaaatcaga
     3001 tgaagaaaag gaagatatcc caattgtttt actgattaac gaaggaagtg ccagtgcctc
     3061 agaaatcatg gcaggagcca tgaaggatac caaaagagca gtcttggtag gaaagaagac
     3121 atttggcaaa ggaatagtac agaaggtcca agaattcggg aacgatggtg aaggaatcaa
     3181 aatgactgtt tcagaatatt tcacaccctc aggaataaac attcacggca taggaattga
     3241 gccggatatt gaagtagaac tgcccgagga tgcagaaggt tatggttatg aatttttcga
     3301 aaccgacacc cagcttaaaa aagccgttga agtattgaac agataaataa atgcaatcct
     3361 gaacaatctt ggatatgctg taaataaaga agagacagaa attacctgtc tcttttgttt
     3421 ttaataaata aaaatatgaa gtatcctatt atcataataa acaaaatcgg gtaaatcatg
     3481 attatatatc caaaatcaac aggcatagcc cacctcctaa taaatcagta cagtcttgtt
     3541 gggatcatcc caaataacgg tttttttaat agcttcgctg acaaatctga ggggtatcat
     3601 tatgcggtca ttcactattt ttgcaggaac atccagggaa ttagcttccc cgttaatata
     3661 ggccgtggtt gagtctgccg gtattattac ttttgttgtg ccgtattcca caactgcaga
     3721 cttggaagat tcctcccatt ttacatcaca gtttaaaact tcaaacacag ctcttaaagg
     3781 gaccatggtc cttccgtttt caataaaagg ccactgatct attgaactga aatctatata
     3841 ttggtcataa accttcacat ttacataatt atccggcttt ttatccgtaa tgtaattaaa
     3901 tgcacctgca ttcagcccgg taatggatat tgtttcgcga agaccttgaa attcaaacaa
     3961 taaattatag tttcccggct ccttgaattt caatgttccg ttggaaatat caattttatc
     4021 cctgtcataa ggataaatat acacatcctt tgtaatgttc acttttttac ttccgtacct
     4081 tgcataaacg ttataggcta cttccgtagc agtattgtaa acatttcttg caaagtccaa
     4141 aagtatttca tcaggtgttt ccttgtattt aataaatagg ttcagaactt tccatttgcc
     4201 tgtaatatca atttcatttg cattatattg aaatgccgat atgaaatcat aattagctaa
     4261 ttgcgaaatt gcattcatta tgctgtcctg agaaattgac ttgtcaaatc tcaatgaaat
     4321 ccttaaaggc tgatgtttta ttttgctggt taacaaatta tttaattcag tttcgtcttt
     4381 taccgaattt tcctcatcgt acatatcaag ccctgtttca accaacaata cgtcatatga
     4441 caattcttta aggtaatcat aatagttctt ggctgatctt ggaaccttca gtccctcatc
     4501 aagagtatgg tctttggata tttctttttc tgcaagcatg taatatgtat aaaacagcgc
     4561 atccctgtca gaaaccgggt catcccatgt tacatcaagg tgaaaccaat aatcgccaag
     4621 cttaaccata ttccatatat gtgcttctcc tccggctgta ccgtaaacca aactgaccgg
     4681 aatgttcagc ctgctcagca tattataggt taaaagggca tatccgttgc agacagaagt
     4741 tcctttcgta agcaagtcat aatctgaata atatatttca cttttatcat atttgctgtt
     4801 taatacaata taatcatgta cagcctttac tttttcgtgg tcattcatgt taggttttat
     4861 aatatcagcc aaaatgccgt caataatttt atctgcttca attctttcat ccctgcttat
     4921 aatatagctt acctttacat ttatgtaagt tacagccgtg ccacggggta tactgtaatt
     4981 gtaatctaca cgactgatgt tggatttcac atagacatcc ttatctacca tgttttttaa
     5041 aacttcatca atgttgtcca gtgagccggt atactttata tcaaaattcg tgttatattc
     5101 tctcatttgc ttaagcatga tattttcaag ctcataatgg ttgcttgctt cattaggcaa
     5161 gccgtaagca ttaagggtga aaatcaatac caggataatt acagcgagaa ccgttttaac
     5221 acttttcatt acatcacctt cctgaccttg tgttatatta tataataaac ttgatgaaaa
     5281 atacagttta attttacaat tacgtgcgca tgtaatataa ttgtcactgt acatccgcag
     5341 tacaaaggat gacagccatg aaccatgcat tataaattag acgcaatcaa agaataaaaa
     5401 gtttcataaa aggagaaata tgtataaaaa ggttttaaac cgaatattgt taagcgaaaa
     5461 gccctctgag gagatatatg aattaattga gaagggcgat atgaataata taatccccga
     5521 acttttaaag cttaaaggtt tcgaccagca taccccttat catgacaggg atgtcctgga
     5581 acacaccatg gctgttgtag acgggattaa gcccaggtta agcttaagaa tggcagcttt
     5641 gctgcatgat ataagcaagc ccgattgctt ttccttggat gaaaagggga taggccactt
     5701 ttacggccac catataagct ctgctcttac aagtgaaaaa attcttcaaa ggcttggata
     5761 tgaagaggaa tttattcagg atgtaacaat tttaataaaa caccactata taaagtatat
     5821 agccaatgtt ataacggaaa agggaataaa aaaatttgtg gaagctgtgg gggcagaaag
     5881 gcttgatgat ttgtttgagc ttatcagggc tgatatggaa ggcaaagctt ccgcaaatca
     5941 tgaagttatc gaacgattaa gagccatgtg caatctctga tgccagtttc catttgatta
     6001 ttcttaaaac agattcattt attctgtctt ctgatatgtc ttggttttta attgaagtca
     6061 aaagtgcctt gtatgatttt tcaaggtctg aaactataag catgtcgcat cctgccttaa
     6121 cggccattgc ctccggtgaa gggatttctg acagggcacc cattgataag tcatctgtca
     6181 ttattattcc cgtaaagtcc atatcatttc gtaaaatatc gattactttt tttgaaagtg
     6241 aagcaggcag ctctgaatca attgattcca caatattgtg tgaaactaaa acactttcag
     6301 ctcctgcatc aatacctgct ttaaaaggca gaaagtcgct tcttaaaaat tcctcataag
     6361 gtcttttatc ataggcagaa ccggtatgag tatctgcatt gtttccatag ccggggaagt
     6421 gttttaaggt tgaggaaatc ttatttttct ttgcttcctc tacaactgct gtaacatatt
     6481 ctgctgtttc ttcggctgat ttaccaaagg ccctgtcaaa aataaaatct ccttcatgag
     6541 ttgacacatc agctacggga cttaagttta aatttatgcc caaggatttt aaaagctttg
     6601 atttttcctc agtttctttt tttatttcct caaaaccgcc cagattgaat aattcttgtg
     6661 gtgatctaaa gggttttgct gcaagattgg ggtttgaact tactcttaca actgttccgc
     6721 cttcctcgtc aacgccgata atcatgggta tgggactgtt attttgataa tattcaatat
     6781 tatttattac ttcttccctc atttttgagt aaaagtcccg gccaaacatt atgaatccgc
     6841 cgggcttcat ggacagataa aattttaaat gttcttcttc agggcacctt gcaagaaaca
     6901 tctgacctat tttttcttct attgtcatat tgtccaataa taataaggct tcttcttcaa
     6961 attcataaaa caagccttta tcttccttgg gaacctcatt cggaatttta ttatcaggag
     7021 tccggggacc ttccaggcat cctgataata acagcaataa gcataaagtt aagattttca
     7081 tattctgctc ctttttatca tcaccttagt aaaaaaagtg caccggagtg cacttttcat
     7141 catcattgct aattaaagct cttcttcagg ttcagtttcc aattgttcaa gcagctggga
     7201 catgtcaggt gcattcaggg cttcttcagg aatttcaaat ttctctgcac tgttgatatt
     7261 taatatctcc attgtcatta aagccttcaa gctctttaaa gcttcaagct cctcagcagg
     7321 tacacctaat gcttccgtca tgccttgcag catagataca accatgccgc ctaaatccat
     7381 ttccatttta accatttcac ctgtagcttc atcaatatat actatgtatg acaaatctcc
     7441 caactcaata tctgcaaatc cttccagcat ttcaaccgcc aatttttcct cttcggttgc
     7501 aggctcaggc attaattctg aagtttcagc aaaaatatcc ttgtatattt ctcctgacag
     7561 tgtatactca agtcttaata aggtttttcc atcttcaaca tatttgccga agtatttaac
     7621 atcttctgta tgctcttcaa tcatttcttt attcttggca attgtttctt cgtcatattt
     7681 cataaggtca gacagcattc ccattgccgt tgtctgctta atccactcaa gctctccggt
     7741 ttcgtcagcc atgcctatat actgcttaat accttcttca tcaattgtca tgtacatttt
     7801 caccatcggg acaaggactt cctcttccat gctcggtaca agcattgaag cagtaatttt
     7861 tgctttgaaa ggctccatga acattgacat atacatattc atatccatgc tgaagggctg
     7921 aacgcttccc gcatcatcgg gaacatccat ggacatatgt atttttgcat taatatcata
     7981 gtttttccaa tcataggatt tttcgtttgc tgcaaaatac aaggctttag catcaacagg
     8041 ctctgtggtc actacaacag tcttcaatgc tccgtcccat tcaacatctg agccgaaagc
     8101 ttcaatgacc gctcttatgg gcagataggt cctgtcattt actattcttg aagcagtatc
     8161 aacatcaatt ttttcgtcat ttttcagtat gtaactttca ccgatcggaa cctttacaac
     8221 tattccatcc tttgttgcaa tcgcggttct tgtttcattg tcccaatcaa cttcagcacc
     8281 gaacttttga aggacagctc tgaatgggac ctgagttctg ttgttttcat cgataaaggg
     8341 gaaacctaac acttcagtaa attcaacctt gtcagaatcg atatttatta tgacaacttc
     8401 atctgcaaaa gctgttacag atgataatgc caa
//
LOCUS       DAOPUT010000063         8295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_8612_length_8295_cov_21.355218, whole genome shotgun sequence.
ACCESSION   DAOPUT010000063 DAOPUT010000000
VERSION     DAOPUT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8612_length_8295_cov_21.355218"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PKK75_06145"
     CDS             <1..236
                     /locus_tag="PKK75_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNZ83214.1"
                     /translation="LDKMKYDTITEGLCCQMLHLGSYDDEPASFEIMGKFCSDRGYER
                     SSLTHREIYISDPRKTQADKLKAVLRFTVKKIK"
     gene            283..1011
                     /locus_tag="PKK75_06150"
     CDS             283..1011
                     /locus_tag="PKK75_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNZ83215.1"
                     /translation="MKSQIWAHRGASFYAPENTLEAFDIAVKQKADGVELDVQLSRDG
                     ELVVIHDETIDRVSDGTGFVKDYNLRELKKFNAGRLFPQYGTCVIPSLEEVYRLLKPT
                     DLIINVELKTGVIFYEGIEEKLILLAKKLDMEDRIIYSSFNHYSLLNLRNINKDVKIG
                     LLVSDIYLGVLDYAVNFGADALHPICHMLKLPGFIEESKSKNMKLHVWTIDNEEEIKL
                     LNEQGIDAIITNKPVTARKAIGRL"
     gene            complement(1038..2249)
                     /locus_tag="PKK75_06155"
     CDS             complement(1038..2249)
                     /locus_tag="PKK75_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNZ83216.1"
                     /translation="MNRNNVLHLLSSVISEKEIIDCIGELVKIKSYQGVENQETEAAE
                     YICRLFESEGIESGLVPVAEGRCNVIGKIKGNGKGKTLLLTGHMDTVPPYDMPNPFEM
                     ENDGNVLTGRGAVDMKGQLACMIFSMIAIKRSNIKLCGDVIFAGVIDEEEKSLGTIDL
                     IEKGLKADAAIVGEPTNLNICTAHRGLEWFEFYFQGKAVHGGCQHEGINAILNASNFI
                     QRIEEKLMPKIIKENHDIIGTSSMNYGTIHGGTQPSTVAGECVLKVDRRWVPGIKYND
                     ILKQYQDVIDELSIEDEKFKCTFKVMETSAMKEGYIHDPMEIDHNHPIIEIAKACTKD
                     VINKEPEITYFPAWSDGGLLSSYAKIPTIIFAPGDLKTAHSVSENLRIDQILPATLIY
                     SLITTEFCNMK"
     gene            2467..3702
                     /locus_tag="PKK75_06160"
     CDS             2467..3702
                     /locus_tag="PKK75_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ83217.1"
                     /translation="MELTGKVLIAQGGGPTAVINQSLVGAVIETRKFPQIKKVYGALY
                     GLSGIVNEDFVDLTRETTNNLEKVADTPSSALFASRDKPDDKYCDDIIKVLMAHDIRY
                     FFYIGGNDSADTVRIVNQKSDEYGYQLRSIHIPKTIDNDLMVNDHCPGFGSAAKFVAQ
                     SFKGINLDVGSLPGVYIGVVMGRNAGFLTASSSIAQKYPDDGPHLIYMPERVFSIDKF
                     ISDVKGVYDKYGKCIVAVSEGIVDEEGKPLVAKLSDNIEIDSHGNIQLSGTGVLGDML
                     AKIIKEKLHIKRVRSDTFGYLQRSFIGCVSDVDQAEAREVGEKAAHYAIWDNVDGSVT
                     IHRTGMYSVDYKLSKLEEVAKYTKRMPDNFINESGNHVTDAYKHYLMPLLGTGMIEVH
                     KLRAPKVDKILNLKKDTSH"
     gene            3837..4433
                     /locus_tag="PKK75_06165"
     CDS             3837..4433
                     /locus_tag="PKK75_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNZ83218.1"
                     /translation="MLLFSFVMGDEPQKVNTKENYIDEKLFRRIGADDMKALDELYHL
                     TERTLYAYILSMVKNHDETLDLMQETYLKIMSSAHLYKPMGKPLAWMFTIARNLYLSR
                     LRKDKRGFNVDSDEMANDLRFSYVTDHDDRIVLESVMKVLEEDERKIVMLYAVSGLKH
                     KEIAEGIGLKLNTTLSKYHRALAKLRKYLTEGSEEHEG"
     gene            4423..5526
                     /locus_tag="PKK75_06170"
     CDS             4423..5526
                     /locus_tag="PKK75_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNZ83219.1"
                     /translation="MKDREILEHIKHGIDKAPIDILDGIKSQKAVKMMKHDEITRQET
                     RKPFKPLMFFASAAAAFLLIFFNIQQFVMPDSRIYIDINPGIEITTNKKDNVIKLEPI
                     NEDAAQIIEDIQYKNNSLKKVTEDIMDSLVVKGYIRGEDEIMLVSVYNGDVNKSKKLT
                     AEMNDTIHNKLGDINKAPILLTQSLEKNNTIEEFANRLGISTGKMTFVRNMIILNPDL
                     KTEDLVDLSLAELIEISRSSGIDIEKIINTGNDERIRMPKTQDQRPADDDYDDYYDDD
                     DFDDHLDDDDYDDDDDDFNDDDVKVKGQRIDEAKAKETALGLVYGNITDFVSDDDEYG
                     IEILAGGYEYNIKIDAYTGEVLEFEKDDIDDDD"
     gene            5648..7279
                     /locus_tag="PKK75_06175"
     CDS             5648..7279
                     /locus_tag="PKK75_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNZ83220.1"
                     /translation="MKKNKKIRISIAAALIAVAAIVGIWFSQPAYTLTMDVNPSIEIV
                     SNRLDKVVEVNPINSDAREMLRDFKIKNRNLKGTIEDLADLMVLKGYISGGKDNLVMI
                     TVSDNAISKESVEKLNNAIVAYLENKQIEATVFNQAVDKKYKDSKTGKDLIAEKISGL
                     DDDADYSRLLNMTLKELIEYAEDRNISSEKLFSRIVGTKPSKETVKEAVKDYKEFIGI
                     EKAKEIALKLVDGNGEIVKFKLDDVYDDDDDNPEYEIEIAADGYEYEIEIDAYTGEVL
                     EFEKDNDDDDKDDLDDDRYDDEDDDYRYDEDNEDDLDDDRYDDKDDRYDEEEDDDLDD
                     DRYDYEDDEDMDDKKVRGERIGEAKAKEIALSWVNGNIVEFESDDEEYKIEILADGYE
                     YEIEIDAYTGEVLEFEKDNDDDDKDDLDDDRYDDEDDDYRYDEDNEDDLDDDRYDDKD
                     DRYDEEEDDDLDDDRYDYEDDEDMDDKKVRGERIGEAKAKEIALSWVNGNIVEFESDD
                     EEYKIEILADGYEYEIEIDAYTGEVLGVDKDEIDE"
     gene            complement(7559..8131)
                     /locus_tag="PKK75_06180"
     CDS             complement(7559..8131)
                     /locus_tag="PKK75_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83221.1"
                     /translation="MKKSNTYETLFRKNNDTDRIIIDVALNDYIEFFHEWDNAVFKKR
                     DIHYELVEFFDTCSEYIPLRKKLEIAISLSTAAASEEKEDQIRVSYHNYYKSLKRLEN
                     RKIKRFLRMSMVLLLISLILLTTYGLYMDVELTGIASRVLMESLLIGGWVFTWEAVHT
                     LFIDIFEPFQRSREIQRFLEADISFIYLSK"
BASE COUNT         2977 a         1256 c         1640 g         2422 t
ORIGIN      
        1 atcttgataa aatgaaatat gatactatca ctgagggact atgctgccaa atgcttcatt
       61 taggaagcta tgatgatgag ccagcaagtt ttgaaattat gggaaaattc tgtagtgaca
      121 ggggatatga gagaagttcc ctcacacacc gcgaaatata tatttcagat ccacgtaaaa
      181 ctcaagctga taagcttaaa gctgtactga gatttactgt gaaaaaaatc aaataaagct
      241 aagaaagtta ttgaatattt aaatattatt ttgggggata ttatgaagtc gcaaatttgg
      301 gcacacaggg gagcaagttt ttatgcacct gaaaatacgc ttgaagcatt tgatatagcg
      361 gttaaacaaa aggccgacgg agtggaattg gatgttcagc tttcaaggga tggagaactg
      421 gttgtaattc atgatgaaac aatagacaga gtaagcgatg gtacaggctt tgtaaaggat
      481 tacaatttaa gagaattaaa gaaattcaat gcaggcaggc tctttcccca atacggtact
      541 tgtgtcatcc cttccctgga agaagtatac aggctattga agcccactga tttaattatc
      601 aatgttgaac tgaagacagg ggttatattt tatgaaggca ttgaagaaaa gctaatcctt
      661 ttggcaaaga aattagacat ggaagacagg attatttatt cttctttcaa ccattatagt
      721 ctgttaaatt taagaaatat taataaggat gtgaaaattg gacttttggt ttctgacata
      781 tatttagggg ttttagatta tgcagtaaat ttcggtgctg atgccttgca tccaatatgc
      841 cacatgctta agctgccggg atttatcgaa gaaagcaagt caaaaaacat gaaacttcac
      901 gtttggacta ttgataatga agaagaaatc aaattgctca atgaacaagg cattgatgcc
      961 attatcacaa ataaaccggt tacggcaaga aaggccatag gcagactata agtataacat
     1021 tccttattta tatttcatta tttcatattg caaaattcag ttgtaatcaa tgaatatatt
     1081 aaagttgcag gcaagatttg atctattctt aaattctcac taacagaatg agctgttttc
     1141 aaatcaccgg gtgcaaatat aattgtagga attttagcat aactgctaag cagtcctcca
     1201 tccgaccaag ccgggaaata tgtaatttca ggttctttat taatgacatc tttagtacaa
     1261 gctttagcaa tttctattat cggatggtta tgatcaattt ccattgggtc atgaatatag
     1321 ccttctttca tcgcacttgt ttccataact ttaaacgtgc atttaaactt ttcatcctct
     1381 attgacagct catctataac atcttgatat tgctttaata tatcattata tttgataccg
     1441 ggaacccatc ttctgtcaac ttttaagaca cattcaccgg caacagtgct gggctgagtc
     1501 cccccatgta ttgtgccgta attcatagaa gaagttccaa taatatcatg attttctttt
     1561 attatcttgg gcatcagctt ttcttcaatt ctttgaatga agtttgaagc atttaatata
     1621 gcgtttattc cctcatgctg gcagcctcca tgtactgcct tgccttgaaa ataaaattca
     1681 aaccattcca aacctctatg ggctgtgcag atattaagat ttgtcggttc tcctacaatt
     1741 gccgcatcag ctttaagccc cttttctata aggtctatag ttcccaaact cttttcttct
     1801 tcatcaataa cccccgcaaa aatcacatca ccgcataatt ttatatttga ccttttgatt
     1861 gcaatcatac tgaaaatcat acaagctaac tgccccttca tgtcaacagc tccacggcct
     1921 gtaagcacat tcccgtcatt ttccatttca aacggattag gcatatcgta tggaggcaca
     1981 gtgtccatat gaccggtcag caataaggtc tttcctttac cattcccctt tatttttcct
     2041 atgacattgc atctgccctc tgccacagga actagtcctg attcgattcc ttcactttca
     2101 aacagcctac atatgtattc agctgcttca gtctcctggt tttctacacc ttgatagctt
     2161 tttattttaa ctagttctcc aatacaatct ataatttctt tttcagaaat tacagaactt
     2221 aacaaatgca atacattatt tctattcata ttttttattc ccttcattct tacttcagta
     2281 atgatttaac tatatgttga ttattgtata aacgttttct cttgtcaata attttttgtg
     2341 cctgaggttt tgcattcttt gtttttttaa tgaaaattat attgaagttt actattatct
     2401 ttgataaaat tgaaatgtat acaatttata aagtttgaat aaaaatcata tatagggggt
     2461 gacaatatgg aattaacagg taaggtgtta atagctcaag gaggaggtcc tacagctgtt
     2521 atcaatcaat ccctggtagg ggcagttata gaaacaagaa aatttccaca aataaagaag
     2581 gtatatggag cattatacgg actttcaggt atagtaaacg aagactttgt agatttgact
     2641 cgagaaacta caaataattt ggaaaaggtt gcagatacac cgtcttcagc tctatttgcg
     2701 tcaagagaca aacctgatga caagtattgt gatgatatta ttaaagtgtt gatggctcat
     2761 gatatcagat attttttcta tatagggggg aatgattcag cagatacagt aagaattgta
     2821 aatcagaaat cagatgaata cggctatcag ctgagatcta tacatattcc aaagacaatt
     2881 gataatgatt taatggtgaa tgaccattgt ccgggattcg ggtctgcagc aaaatttgtt
     2941 gctcaatctt ttaaaggaat aaacttggat gtgggttctc ttcccggtgt ttatatcgga
     3001 gtagtcatgg gaagaaatgc agggttttta acagcttcat cttcaattgc gcaaaagtac
     3061 cctgatgatg gtccccattt gatttatatg cctgaaaggg tattcagcat cgataagttc
     3121 atatccgacg taaagggagt ttatgataaa tacggtaaat gtattgttgc agtgtctgaa
     3181 ggtattgttg atgaagaggg gaaacccttg gttgcaaaat tatctgataa tattgaaatt
     3241 gatagtcatg gcaacatcca gctgtccgga acaggtgttt tgggtgatat gctggctaag
     3301 attataaaag aaaaactaca cataaagaga gtcagaagcg atactttcgg ttatttacaa
     3361 aggtccttca taggatgcgt atccgatgta gaccaggctg aggcaaggga ggtaggcgaa
     3421 aaggctgctc attatgcaat atgggacaac gtggatggtt ctgttaccat ccatagaaca
     3481 ggaatgtatt ctgttgacta taaattatcc aaacttgagg aagttgcaaa atacacaaag
     3541 cgaatgcccg ataactttat aaatgaatcc ggaaatcatg ttaccgatgc atataagcat
     3601 tatttaatgc ctctgctggg gacaggaatg atagaagttc ataaattgag ggctccaaaa
     3661 gttgataaaa tattaaacct caaaaaagat acttcacatt aaaaattgag ctatgtggta
     3721 atatcaattg ataaataaac ggcaggcaat acctgccttt taaatttatg aaaaatatat
     3781 ttttaaatgc aataaaacaa tctttattgg cgttatctaa acagaaggag attggcatgc
     3841 tattattcag ctttgtgatg ggtgatgaac cacaaaaggt aaatactaaa gaaaattata
     3901 tagatgaaaa gctttttagg cgtatcggtg ctgatgacat gaaagccctg gatgaattgt
     3961 accatctgac agagcgaaca ctatacgcct atattttgtc aatggttaag aaccatgatg
     4021 aaaccctgga cttaatgcag gaaacttatc ttaagatcat gtcatcagcc cacctttaca
     4081 aacccatggg caagcccttg gcctggatgt ttaccattgc caggaacttg tatctttcta
     4141 gattaagaaa ggataagagg gggtttaatg tggattcaga cgaaatggca aatgatttaa
     4201 gattttctta tgtaaccgac catgatgaca gaattgtttt agagtctgtt atgaaggtat
     4261 tagaagaaga tgaaagaaaa atagttatgc tgtatgctgt atcagggctt aagcataagg
     4321 aaattgctga aggcataggg cttaagctca acacaactct ttcaaagtac catagagcat
     4381 tggcaaaatt aagaaaatat cttacagagg ggagtgaaga acatgaagga tagggaaatt
     4441 cttgaacata ttaaacacgg aatagacaaa gcacccattg atattttaga cggcattaag
     4501 agtcaaaagg cagtaaagat gatgaagcat gatgaaatta caaggcagga gacaagaaag
     4561 ccctttaaac ccctaatgtt ctttgcctcg gctgcggcgg catttttatt gatatttttc
     4621 aacattcagc aatttgtaat gccggacagc agaatttata tagacattaa tcccggtatt
     4681 gaaattacca ccaataaaaa agacaatgta ataaaactag agcccataaa tgaagatgca
     4741 gcacaaatta tcgaggatat ccaatataaa aataattccc ttaagaaagt aactgaagac
     4801 attatggatt ctttagtcgt taagggctac atccgtgggg aagacgaaat tatgcttgta
     4861 tcagtctata acggagatgt taataaaagc aaaaaactga cagctgaaat gaatgatact
     4921 attcataata aacttggtga tataaacaag gcccccatat tattgactca gtctctggaa
     4981 aagaataata caattgaaga atttgcaaac agattaggta tatccacagg caaaatgact
     5041 tttgtcagaa atatgataat acttaatccg gatcttaaga cagaagatct tgtagattta
     5101 tctcttgcag aactgattga aatctcacgc agttccggaa tagatataga aaaaatcata
     5161 aataccggaa atgatgaaag aatcagaatg ccgaaaactc aagaccaaag accggctgac
     5221 gatgattacg atgattatta tgatgacgat gattttgatg atcaccttga cgatgatgat
     5281 tatgatgatg acgacgatga cttcaatgat gatgatgtaa aggtaaaagg tcaacgcata
     5341 gatgaagcaa aagcaaaaga aacagcttta ggcttggttt atggaaacat aacggatttt
     5401 gtttcggatg atgatgaata tggaattgaa attctagcag gtggttacga atataatatc
     5461 aaaatagatg cctataccgg cgaagtttta gaatttgaaa aagacgatat agacgatgat
     5521 gattaagatg atgattatta ggatgctaaa aaaatataaa aaataaaaaa atttaaaaat
     5581 tgatgcaata aaattaagct cccctgcgtt aactgaatat aagagagatt aattaggagg
     5641 taaaagaatg aaaaaaaata agaaaataag aatatcgata gcagcagctt tgatagcagt
     5701 agcggcaatt gtggggattt ggtttagtca accggcttat acactgacta tggatgtaaa
     5761 tccaagtatt gaaatagtat caaacagatt agacaaagta gtagaagtaa atccgataaa
     5821 cagtgatgca agagaaatgt tgagggattt caagattaag aacaggaatc taaaaggaac
     5881 aattgaagat ttagcagact taatggtact gaagggatat atttcaggcg gaaaagataa
     5941 tttggtaatg ataaccgtaa gcgataatgc tatcagtaaa gagtccgtgg aaaagctcaa
     6001 taatgcaata gttgcatatt tggaaaataa gcagattgaa gcaacagtat ttaaccaagc
     6061 tgtggataaa aagtataaag acagtaagac tggcaaggat ctaatagcag aaaagataag
     6121 cggattggac gatgacgcag attacagcag gctcttaaat atgaccttga aggaattaat
     6181 agaatatgca gaagatagaa acatttcatc agaaaagctg ttttcaagga tagtgggaac
     6241 aaaaccaagc aaagagactg tcaaagaggc ggtaaaagac tacaaagaat ttattggaat
     6301 tgaaaaagca aaagaaatag ctttaaaatt agtagatggg aatggagaaa ttgttaagtt
     6361 taaacttgat gacgtatatg atgacgatga cgataatccc gagtatgaaa ttgaaatagc
     6421 tgctgatggt tacgaatatg aaatcgaaat agatgcatat acaggtgaag tattagagtt
     6481 tgaaaaagat aatgatgatg acgataaaga cgacctagat gatgacagat atgacgatga
     6541 agacgatgac tacagatacg atgaagacaa tgaagacgac ctagatgatg acagatatga
     6601 cgataaagac gacagatatg atgaagaaga agatgatgac ctagatgatg acagatatga
     6661 ctatgaagac gatgaagaca tggacgataa gaaagtcaga ggagaacgta taggagaagc
     6721 aaaagcaaaa gaaatagctc tgagctgggt taatggaaat attgtggagt ttgaatcaga
     6781 cgatgaagaa tataaaattg aaattcttgc ggatggttac gaatatgaaa tcgaaataga
     6841 tgcatataca ggtgaagtat tagagtttga aaaagataat gatgatgacg ataaagacga
     6901 cctagatgat gacagatatg acgatgaaga cgatgactac agatacgatg aagacaatga
     6961 agacgaccta gatgatgaca gatatgacga taaagacgac agatatgatg aagaagaaga
     7021 tgatgaccta gatgatgaca gatatgacta tgaagacgat gaagacatgg acgataagaa
     7081 agtcagagga gaacgtatag gagaagcaaa agcaaaagaa atagctctga gctgggttaa
     7141 tggaaatatt gtggagtttg aatcagacga tgaagaatat aaaattgaaa ttcttgcgga
     7201 tggttacgaa tatgaaatcg aaatagatgc atatacaggt gaagtattag gggtcgataa
     7261 ggacgaaata gatgaatgat gacgacgaca gatagataat gatgacaata ataacaatga
     7321 taataattta aatacagaga aatcgtgaga ctaatacagt ctcacagttt tcttgcagat
     7381 ttttatgctg tttattaatt gcttgtataa atttggcaaa atttgtaata ttatgaatat
     7441 aatcaatgta gcaaagaacc ggtctttgtt attgaatagt ctaaattaac taaagcgccc
     7501 tgtaataata gcagggcgct ttaattttca atattttatt ttaatattat atgctgtgtt
     7561 atttggacag gtagataaag cttatatctg cttccaaaaa acgctgtatt tcacgacttc
     7621 tttgaaaagg ttcaaagata tcaatgaaga gagtgtgcac agcttcccat gtaaaaaccc
     7681 agccgcctat aagaaggctt tccatcagaa ctcttgaagc aataccggtt aattcaacat
     7741 ccatatataa accataagta gttaacagta taagagatat aagcagtaaa accattgaca
     7801 ttctcaaaaa tctttttatt ttacggttct ccaagcgttt taaagacttg taatagttat
     7861 gatagcttac gcgtatctgg tcttcttttt cttcactggc agcagctgta ctcaaggaaa
     7921 tggcaatctc aagctttttt cttaaaggga tatattctga gcatgtatca aaaaactcta
     7981 ccagctcata atgaatgtcc cgctttttaa aaacagcatt atcccattcg tgaaagaatt
     8041 caatataatc attaagggca acatcgataa tgatacggtc cgtatcattg ttttttctaa
     8101 ataaagtttc ataagtattt gactttttca taatttgcct ctaaaaatat ttgcttccct
     8161 atttgtcatt attatatcac aacaaaatat ccatggatac ataatttttg ccggcagtta
     8221 caaataaata gtattcctga attgaaaaca catgaaaaat atggtataat agaagaaaac
     8281 caggttatta aaaag
//
LOCUS       DAOPUT010000064         8226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_8704_length_8226_cov_21.284543, whole genome shotgun sequence.
ACCESSION   DAOPUT010000064 DAOPUT010000000
VERSION     DAOPUT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8704_length_8226_cov_21.284543"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PKK75_06185"
     CDS             complement(<1..1196)
                     /locus_tag="PKK75_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNZ83222.1"
                     /translation="MDYIIISMLIILIAVNLVLLIRQLKNNSKDQIISELKINIRENN
                     LDLIDSVNGKIAESEHKQLRELLTNKLETFERIDKNTNRQTESFLNFSADTSKTLNDN
                     FTSLNERVGQNLDRINQRVEERLNEGFEKTNKTFINILERLSRIDEAQKKIDSLSTNI
                     VSLQDVLTDKKSRGTFGEVQLNHILSVVFGEKNDKIYELQKTLDNSMIADAVLHIPEP
                     VGMLCIDSKFPLENYQRMIDLNNSESERRKYARDFKINVKMHIKDIASKYIIQGQTSD
                     QAIMFIPAEAIFAEINAYHQDLLDYAGENRVWITSPTTLMSVLSTVQVVLRNIEREKY
                     AGIIHEELNKLGKEFKLYKDRWESLSKNIKRVSDDVDKINITSNKIERKFDRISKVEM
                     NCENEI"
     gene            complement(1463..2476)
                     /locus_tag="PKK75_06190"
     CDS             complement(1463..2476)
                     /locus_tag="PKK75_06190"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005977447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNZ83223.1"
                     /translation="MKKYNVAILGATGAVGQEMLKVLGERNFPINNLKLLASKRSEGA
                     LVEFKGKEYAVEEAKESSFENIDVVLCAAENDISEKLSPAAVKAGAVVVDNSSAFRLF
                     DSVPLVVPEVNGEDVKNHKGIISNPNCSTTIALTAINELNKYSKIKRMIVTTFQAVSG
                     AGVNGIKELDQQIKDISEGKEVEINTFQYQIAYNVIPQIGSFDEAGYSQEEMKLQKEG
                     RKILHNPDLKVNCTCVRVPVYRSHSESITIETEKEITPEKARELLSKAAGVKLIDDVK
                     NKKYPMPLDTSDQDLIYVGRIRKDISAENSLVLWCSGDQIRKGAATNAVQIAEVLAKE
                     NLI"
     gene            complement(2732..4510)
                     /locus_tag="PKK75_06195"
     CDS             complement(2732..4510)
                     /locus_tag="PKK75_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNZ83224.1"
                     /translation="MFKKAKYAILLLLLTVLLTSCTESADADVDTTTPGASNGDVEIV
                     PSEPEISEEEKAKLELEKRKAEREELEKQRKEQLGEFYVPLIPVGEERDKKTVEVKAL
                     YVTAHTAENELNKENIDAYGAYVKALEEKDSQKARELFDSAEKANKFERIVGIAAATE
                     INGLVINVKDDNGFITYESNVEIVQDMNKDTPVPIKDIHKMLKVLKEYDIYTIGRIVA
                     FKDKNFAVKNPDHSIQLKSGGTWLDPNSGGIPWINPFDQYVWDYNVAIAKEASLVGFD
                     EIQFDYVRFPDGAKTYNPITEFPGRNGRDKDEAIADFLEYSRKELEPYNVNLGADVFG
                     LITRTWDDYPEDIGQTWILIGEHVDNICPMVYPSHYSTGWYNLENPNAYPYLVVKGAM
                     EEAIEKNSSMEKPPVVRPWIQDFDWAGIVYGPDKVRDQIIAAKELGIFEYMIWNPSNV
                     YDPYAFMLTEKEKAVVYPLDKGELDYKEKSPVDGAEKFLTGMLQGKYSYTYLMTPLAY
                     RAKDFDEFSKLQEESYIKLLNFKVYSYTMDAYDNNKANVSLYYKIEVKKGDTTEIIEK
                     DNVVWEAVRELNIWKIKADWDTGDVS"
     gene            complement(4700..5200)
                     /locus_tag="PKK75_06200"
     CDS             complement(4700..5200)
                     /locus_tag="PKK75_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83225.1"
                     /translation="MLLKSKDTAYLGVLLGLNQLFIILSSVIETSTIILMAAGALVVG
                     VVVVEFGGKTGILFYIASCILGFILTFNKVEIITYICFFGIYSIIKHYIETKVLNRYI
                     SYAVKIASFNILILLTYLTARLFINFPLQWLMILSAQVLFLIYDYAFTTFINVYINSI
                     KPKLRR"
     gene            complement(5191..5793)
                     /locus_tag="PKK75_06205"
     CDS             complement(5191..5793)
                     /locus_tag="PKK75_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNZ83226.1"
                     /translation="MKDPYEILGLNRGATKEEIKSAYRKLAKMYHPDMNENNPPHLQE
                     LAEEKFKEIQWAYDEIMNGNADSAGYGSTYTGNNSRSTGDLYSVRQYVNSGRFQEALS
                     ILRRTRVRNAEWNFLTGVCYVGLGSINQGMQHIQTAVNMEPANMEYQNYLNQVYNMQR
                     SYQQRAYNYGGGSNSVDCCTQLICADCLCECLGGDLISCC"
     gene            complement(5777..6604)
                     /locus_tag="PKK75_06210"
     CDS             complement(5777..6604)
                     /locus_tag="PKK75_06210"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HNZ83227.1"
                     /translation="MFGYVNINKMELKFKEYYSYKGYYCGLCKSLKRKYGNVSRLTLN
                     YDMTFLILLLSSLYEPDNKISNERCIVHPGRKQFVIQNDITDYAASLNVMLSYYNMLD
                     NWEDDRDYKSLAAARLLESKFIKSSSVLCDLTKTVKLRLNNISMLEKQNIDDIDAVSN
                     EFGRLMEEMFLYKKDHWEPSLRKIGFYLGKYIYFIDAFDDMKKDEVKSSYNPFNKLNI
                     ENKEEYARSLLMLNLSLLSDEIEKLPLAQDKGIIDNIIYSGMLNKFNNKENTNERPV"
     gene            6926..7573
                     /locus_tag="PKK75_06215"
     CDS             6926..7573
                     /locus_tag="PKK75_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNZ83228.1"
                     /translation="MNTNKKEITSALTHLGGAIFGVIALCLLLNMSFRMKSLTAAVSF
                     LIFGLSMILLYSTSCVYHFIDSSKKKAKVIMRKLDHIMIFVLIAGTYTPVCLLVLDKN
                     IGLKLLALVWSITIIGAFIKIFWINAPRWVSAGLYLGMGWISVFVFMPLVRSMSASGL
                     FWLISGGLLYTIGGVIYGLKKPNPDKTWFGFHELFHVFVLAGTFCHFMMMYFYVA"
     gene            complement(7586..7795)
                     /locus_tag="PKK75_06220"
     CDS             complement(7586..7795)
                     /locus_tag="PKK75_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83229.1"
                     /translation="MISIKLSGDNGVKSIELEYKDVTIDEIITELNIDKHHIGAVLVN
                     CIPKKFSDKVEDNAEIYFLPILSGG"
     gene            complement(7792..>8226)
                     /locus_tag="PKK75_06225"
     CDS             complement(7792..>8226)
                     /locus_tag="PKK75_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ83230.1"
                     /translation="MPAYDPRAIKAMGVTFATSPMGADHTYGPMIKTNDYIGASKDAQ
                     IMMAKIDCLGVCMFARQAIAKHMDLLVELVNSVHGWELDMDWLDDTALEAIKDEHKFN
                     ELAGFREEDYRMPKAFTERKIESTEQVFDINQDELDKLRSWL"
BASE COUNT         2248 a         1668 c         1334 g         2976 t
ORIGIN      
        1 tcaatttcat tctcacagtt catttcgacc ttggatattc tatcgaattt tctttcaatt
       61 ttattggaag ttatgtttat tttgtccaca tcatctgaaa cccgctttat atttttagac
      121 agggactccc acctgtcctt gtagagctta aactctttcc ctaatttatt aagctcctca
      181 tgaattattc ctgcgtattt ttccctttct atatttctta gaacaacctg tacggttgac
      241 aaaactgaca tcaatgttgt tggagaagtt atccaaaccc tattctcacc tgcataatcc
      301 aaaaggtcct gatggtatgc atttatttct gcaaatattg cttctgcagg aataaacatt
      361 attgcctggt ctgatgtttg tccctgaatt atgtacttgg aagctatatc tttaatatgc
      421 atcttaacat ttattttaaa atctcttgca tattttcttc tttcactttc ggaattgttc
      481 aaatcaatca tgcgctgata attttcaaga ggaaattttg aatctatgca aagcattcca
      541 accggttcgg gtatatggag cacagcatct gcaatcattg agttatccaa tgttttttga
      601 agttcatata tcttatcgtt cttctctccg aaaaccaccg acaaaatgtg attgagctga
      661 acttctccaa aggttcctct gcttttttta tctgttaaaa catcctgcaa tgaaacaata
      721 tttgtagaaa ggctgtctat tttcttttgt gcctcatcta ttctcgataa tctttcaaga
      781 atgttgataa atgttttgtt tgttttctca aaaccttcat tcagtctttc ttcaaccctt
      841 tggtttattc tgtcaagatt ctgccctacc ctttcattca aagaagtaaa gttatcattc
      901 agagtctttg aggtgtcagc actgaagttt agaaaggatt cggtctgtct attggtgttt
      961 ttgtcaattc tttcgaatgt ttccaacttg tttgtcaaca gttccctcag ctgcttatgc
     1021 tcagattcgg caattttccc gttcacagaa tcaatcaaat ctaaattgtt ttctcttata
     1081 ttaattttaa gctcagaaat tatttggtcc tttgaattgt tttttaactg ccttataagt
     1141 aaaacaagat ttacagcaat cagaattata agcattgata taataatata gtccataaaa
     1201 cctccttcgt aatgggtacg cttaaaattg cgctcatcgg cgagctgtac gattttgatg
     1261 gtgtgttccc tttactgata tattttataa cataatcgaa catttgtcca attaaatata
     1321 tgaaactcaa aatcctttga tggcagcacc gcgggttgag gtctctttta cggcataata
     1381 cttcataaaa aagagacttt tacctgtctc cttttttgtt tgaatatttg tgtaagtttc
     1441 tctcaactta tcaatgataa aatcagatta gattttcttt tgccaatact tccgctattt
     1501 gtacggcatt tgtagccgcg ccttttctta tttggtcccc gctgcaccat aataccagtg
     1561 aattttctgc actaatatcc tttcttatcc tgcctacata tattaaatcc tggtctgatg
     1621 tatcaagagg catgggatat tttttatttt tcacatcatc aatgagcttc actccggctg
     1681 cctttgataa aagctctctt gccttttcag gagttatttc cttttcagtt tctatggtga
     1741 ttgattcgga atggcttctg taaacaggaa ccctaacgca tgtacagttt acctttaagt
     1801 caggattgtg aaggattttt cttccttctt tctgcagttt catttcctcc tgtgaatatc
     1861 ccgcttcatc aaagcttcct atctgaggta ttacattgta tgcaatctga tactggaatg
     1921 tatttatttc aacttcttta ccttcgctga tatccttaat ctgctggtca agctccttta
     1981 tcccgtttac tccggcgcct gatactgcct ggaaggttgt aactatcatc cttttaattt
     2041 tagaatattt atttagttca tttattgcag tcaatgcaat ggttgttgag cagttggggt
     2101 ttgatattat tcccttatgg tttttaacat cctctccgtt aacttcaggc acaacaagag
     2161 gaacactgtc aaataatctg aatgcacttg aattatcaac tacaaccgca cctgccttta
     2221 cagcagcagg agacaatttt tcgcttatat cgttttcagc tgcacaaagg accacatcta
     2281 tattttcaaa agatgattct tttgcttctt ccactgcata ttcttttcct ttgaattcaa
     2341 ctaaagctcc ttcacttctt ttgcttgcca gcagctttag attgtttatg ggaaaattcc
     2401 tctcccccag gaccttcagc atttcttgcc ctactgcacc tgttgcccct aatattgcca
     2461 cattgtattt tttcataccc gtcctcgcct tctgcaggtt aacctgctta aatatataaa
     2521 aataattata ccaataaatc taagcttgtc aagctttttg gttttatttt gtgcacatat
     2581 gttttaatct aacgtacacc attattgttg tatagcaaaa aatagtaacc cttttaaatg
     2641 aatatgctca aggtttcaag taatgccaaa aatatttgtt taaaagcaaa ctttaagaca
     2701 ttatattcct ctcgtttcag gtatgaccgg tttaagatac atccccggtg tcccagtctg
     2761 cttttatttt ccagatgtta agttccctta cagcctccca gacaacattg tccttttcaa
     2821 ttatttctgt agtatcacct tttttaactt caattttata gtataggctt acatttgctt
     2881 tattgttgtc ataagcatcc attgtataag aatatacttt gaagtttaac agcttaatat
     2941 aactttcttc ctgaagtttt gaaaattcat caaaatcctt agccctgtat gcaaggggag
     3001 tcattaaata tgtgtagctg tactttcctt gaagcatgcc ggtcaaaaat ttctctgctc
     3061 cgtctacagg tgatttttct ttataatcaa gctcgccctt atccagagga taaacaactg
     3121 ccttttcttt ttcagtaagc ataaatgcat aggggtcata aacattgctt ggattccaaa
     3181 tcatgtattc aaatatgcca agctccttgg ctgctataat ttggtctctc accttatcgg
     3241 gaccgtatac aattcctgcc cagtcaaagt cttgaatcca aggtcttaca accggcggct
     3301 tttccatgga tgaatttttt tcaattgctt cctccatggc cccttttaca actaagtaag
     3361 gataggcatt tgggttctcc aaattgtacc agcctgtgga ataatggctg gggtaaacca
     3421 tggggcagat attatcaaca tgctctccta tcagtatcca ggtctggcct atatcctcag
     3481 gataatcatc ccaggtcctg gttattaagc caaatacatc ggcacctaaa tttacattgt
     3541 agggctctaa ttcctttctt gaatattcca aaaagtcagc aatcgcttca tccttatcac
     3601 gtccgtttct tccggggaat tccgtaatgg gattatatgt tttagccccg tccgggaaac
     3661 gcacatagtc aaactgtatt tcgtcaaagc cgacaagcga tgcttccttg gctattgcca
     3721 cattgtaatc ccaaacatac tggtcgaagg gatttatcca gggaatacct ccgctgttag
     3781 ggtcaagcca tgttcctccg gacttgagct gaatggagtg gtcaggattt ttgacagcaa
     3841 aattcttgtc cttgaaggca acaattctac ctatggtata gatatcatac tccttcaaca
     3901 ccttaagcat tttatggata tcctttatcg gaacaggtgt gtctttattc atgtcctgaa
     3961 ctatttccac attgctttca tatgttataa agccgttatc atccttgaca tttataacca
     4021 ggccgtttat ttcagtagca gcagctattc ctacaattct ttcaaattta tttgcttttt
     4081 cagcgctgtc aaacaattct cttgcctttt gggaatcttt ttcttcaaga gctttcacat
     4141 atgcaccgta tgcatcgatg ttctccttgt ttaattcatt ttctgccgtg tgggctgtta
     4201 cgtacaatgc tttaacttca acggtctttt tatccctttc ttcgcctacc ggaataaggg
     4261 gtacataaaa ttctcccaat tgctcttttc tttgtttttc cagctcttct ctttcagctt
     4321 ttcttttttc cagttctaat tttgcttttt cttcttctga tatctcaggt tctgaaggta
     4381 caatttcaac gtctccgtta cttgctccgg gggttgttgt gtcaacatct gcatcagcgg
     4441 attcagtaca tgatgtcaat aacactgtga gtaataataa taaaatcgca tattttgctt
     4501 ttttaaacat aaatcctcct ataaattaat aggtgcagat gacaccttta atatatggta
     4561 tcaattctca attcaaatac cacttttaat tttagcataa tctattttta taagcaatgg
     4621 ataatacagg ctttttcatt tttcgacaaa gactggagcc aatacccgca ggcagtacag
     4681 aagtgaaatt ttacacattt catcttctta attttggttt tatagaattg atatatacat
     4741 tgataaatgt tgtgaaggcg tagtcatata ttaggaacaa tacctgggct gataaaatca
     4801 tcagccattg gaggggaaaa ttaataaaaa gtcttgctgt caaatatgtt aacaatatta
     4861 aaatattaaa agatgcaatt tttactgcat aggatatgta tctatttagt acttttgttt
     4921 caatatagtg cttaataatg ctgtaaatac cgaaaaaaca tatgtatgta ataatttcaa
     4981 ccttgttgaa ggttaaaata aaacctaata tgcatgatgc aatgtaaaac aatattcctg
     5041 tttttcctcc gaattcaacc actacaactc ccacaaccaa tgctcctgcg gccatcagaa
     5101 ttatggtgct tgtttcgatt actgatgaaa gtattataaa cagttgattt agtcctaaaa
     5161 gcacacccag gtatgccgtg tccttgcttt ttagcagcat gaaataaggt ctccccccaa
     5221 acattcacaa aggcagtctg cacagataag ctgagtacag caatcaacag agtttgagcc
     5281 ccctccgtaa ttgtatgctc tttgctgata tgagcgctgc atgttgtata cttgatttaa
     5341 atagttctgg tattccatat ttgcaggctc catattgaca gcagtttgaa tatgctgcat
     5401 gccttgattg atagacccta agcctacgta gcaaacaccc gtcagaaaat tccattctgc
     5461 atttctaacc cttgttcttc taagaatgct cagagcttcc tgaaatcttc ctgaattgac
     5521 atattgcctt actgaatata aatcaccggt gcttcttgaa ttgttgcctg tatatgtgga
     5581 gccgtagcct gcgctgtcgg cgtttccgtt cattatttca tcataagccc attgtatttc
     5641 cttgaatttt tcctctgcca attcctgcaa atgaggagga ttgttctcgt tcatgtcagg
     5701 atgatacatc tttgctaatt ttctgtaggc gcttttaatc tcttccttgg ttgctcctct
     5761 atttaagcct agtatttcat acgggtcttt catttgtatt ctccttatta ttaaatttat
     5821 tcaacatccc tgaatatata atattatcaa ttattccttt gtcctgagcc aagggcagtt
     5881 tctctatctc atcgcttaaa agcgacaaat taagcattaa gagagacctg gcgtattctt
     5941 ccttgttttc tatatttaat ttattaaagg gattatatga gctcttcact tcatccttct
     6001 tcatatcgtc gaaggcatca ataaaatata tatatttacc cagataaaag ccaattttcc
     6061 ttaagcttgg ctcccaatgg tcctttttgt ataaaaacat ttcttccatc aagcggccaa
     6121 actcattgga tacagcatca atatcatcta tattttgttt ttcaagcatg gaaatattat
     6181 tgagcctaag ctttaccgtt tttgttaaat cacaaagaac agagcttgat ttaataaatt
     6241 tgctttccaa cagcctggct gctgccaatg atttgtagtc cctgtcatct tcccagttat
     6301 caagcatatt gtaatatgaa agcattacgt ttaggctagc agcataatct gttatgtcgt
     6361 tttgtataac aaactgtttc ctgcctggat gaacaatgca tctttcatta gaaattttgt
     6421 tgtccggttc ataaagggag cttaataaaa gtattaaaaa tgtcatatca taattcaatg
     6481 taagccttga aacattcccg tactttcttt ttaagctttt gcacaggccg cagtaataac
     6541 ccttgtagct gtaatattcc ttgaatttca attccatttt attaatgtta acgtatccga
     6601 acattgactc actcatttct tatgttgtta ttaattgtat caagactgtc ttgttttatc
     6661 aataaaaatt ttggggataa actccaggct aaaaactccc tgagcaaaag aaattacacc
     6721 tttatatact acatccgaat aattaaaata gtattaataa acaatgaatt gccgatttaa
     6781 tatagccctt cctgttcagg aaaaatcttt gtttgtagga agaatgtctc cagatgatgt
     6841 ctcttaaaaa tgctgttatg gcatcttata attgacattg ttgccattaa agaatattat
     6901 agtgtaaagt atcaagggag gaaatatgaa tacaaataaa aaagaaatca ccagcgcttt
     6961 aactcacctt gggggagcaa tttttggagt aattgctttg tgtctgttgt tgaatatgtc
     7021 ctttaggatg aagagcctga ctgctgcagt ttcgttcttg atcttcggtt taagcatgat
     7081 acttttgtat tccacttctt gtgtttacca ttttattgac agttcaaaga aaaaagcaaa
     7141 agtcataatg aggaaactgg accacataat gatttttgtt ttaattgcag gtacctatac
     7201 accggtatgc cttttagtat tggataaaaa cattggactt aaattactcg ctcttgtatg
     7261 gtcaattaca ataataggag catttataaa aatattttgg attaatgccc ccagatgggt
     7321 gagtgcagga ttatatctgg gaatgggatg gatttcagtt tttgttttca tgcccttggt
     7381 aagaagcatg tccgcttcag gattattctg gcttatatca ggtggattat tatacactat
     7441 cggaggagtg atttacggac tgaaaaaacc aaacccggat aaaacatggt ttggttttca
     7501 cgaattgttt catgtatttg ttttggcagg aaccttctgt cactttatga tgatgtactt
     7561 ttatgttgcc tgagaatacg gctttttagc ccccgcttaa aataggcagg aaatatattt
     7621 ctgcattatc ttccacctta tctgaaaatt tctttggaat acagttgaca agcacggcac
     7681 cgatgtgatg cttgtcaata tttaattcag tgattatttc atcaattgta acatccttat
     7741 attcaagctc aatactcttt acaccgttat ccccggacag ttttatgctt atcatagcca
     7801 tgacctcagt ttatccagct catcctggtt aatatcaaaa acctgttctg tgctttctat
     7861 ttttctctcc gtaaatgcct ttggcatgcg gtaatcttct tcgcgaaatc cggcaagctc
     7921 attaaatttg tgctcgtctt ttatggcttc caatgccgta tcgtcaagcc aatccatgtc
     7981 cagctcccag ccatgaactg aatttaccaa ttcaacaagt agatccatat gttttgctat
     8041 tgcttgtctt gcaaacatgc atactcccaa gcagtcgatt tttgccatca taatttgtgc
     8101 atcctttgaa gcaccgatgt agtcattggt ctttatcatg ggaccatagg tatggtctgc
     8161 acccatgggt gaggttgcaa aggtaactcc cattgctttt attgctctgg gatcatatgc
     8221 aggcat
//
LOCUS       DAOPUT010000065         8162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_8791_length_8162_cov_17.760947, whole genome shotgun sequence.
ACCESSION   DAOPUT010000065 DAOPUT010000000
VERSION     DAOPUT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8791_length_8162_cov_17.760947"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..314)
                     /locus_tag="PKK75_06230"
     CDS             complement(54..314)
                     /locus_tag="PKK75_06230"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNZ83231.1"
                     /translation="MEVYIITFLHGNYAVAAFNRLQGYGVKNIQLVQTPFSIKNECDI
                     CIKTNNQKTLDIVLNECRGRFPVSNVYSKINKNGSFVYKLLP"
     gene            complement(383..676)
                     /locus_tag="PKK75_06235"
     CDS             complement(383..676)
                     /locus_tag="PKK75_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83232.1"
                     /translation="MKAYDLINKVEMDVTTQDLINLMTEANRQIDLILYDKKTDDDGY
                     LTWDAEHWTIVTAKKFMRTYSLGDRQLRDYTSHNIYDLKNDFKPEEAKEIQIN"
     gene            complement(707..1204)
                     /locus_tag="PKK75_06240"
     CDS             complement(707..1204)
                     /locus_tag="PKK75_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83233.1"
                     /translation="MYKLFGKITDPNIENIINDKIDFQNLNKDLAYDVHVDFYNPDLE
                     EDMLNSDVSFEIKKDHYRFIKKIRTLFESNQIKINEFYMMGTISDLKEDEINITILKS
                     KADNKKNTVWPCKEIFLFEDQKNRLDLLLFSNQISEEDYESNLEFLRDELNIYENDEE
                     HKYIN"
     gene            complement(1216..2520)
                     /locus_tag="PKK75_06245"
     CDS             complement(1216..2520)
                     /locus_tag="PKK75_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNZ83234.1"
                     /translation="MNIFEMQYQNSIKKEGPLAERMRPLNLNEFVGQEHIVGQGKILN
                     RAIEADKLTSAIFYGPPGTGKTTLAKIIANTTNKNFTTLSAVTSGIKDIKEKISYAID
                     ELSIYNKRTILFIDEIHRFNKAQQDALLPYVENGTIILIGATTENPYFEVNPALISRS
                     LIFEFKSIDIEDIKAVVLKSLADKRGYGNRKVEIDEDALTHIAEYANGDIRSALNALE
                     LGVETTCETDGMIKINLDIASECIQRKKIDYDKNGDNHYDTISAFIKSMRGSDPDAAV
                     YWLAKMIKAGEDPKFIARRIIICASEDVGNADPRALEVAVNAFRAVEIIGFPEGRITL
                     AHAAIYTACAPKSNSVVIAIDKAMEAVEKESHEVPAHLKDSHYKGAEKLNRGIEYKYP
                     HDYEKFYIKQQYMPETIKTIFYEPKESGYESKIKKFLEYLRQ"
     gene            complement(2649..3071)
                     /locus_tag="PKK75_06250"
     CDS             complement(2649..3071)
                     /locus_tag="PKK75_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNZ83235.1"
                     /translation="MDNVGRIEKIDGNMAVISVKRVSACGDSCKSCSSACKVNSVILE
                     TDISDVEDQINVGDFVEINTENEVMLKHIAVLYGLPLVLMLATVFIFQMIISGPNKDI
                     ISAIASLFSLIISFYLLKAYDKREMKKNVLKFTIGKKL"
     gene            complement(3091..4863)
                     /gene="aspS"
                     /locus_tag="PKK75_06255"
     CDS             complement(3091..4863)
                     /gene="aspS"
                     /locus_tag="PKK75_06255"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNZ83236.1"
                     /translation="MDTMGTLRRTNMCGELNKDHIGSEVVLMGWVQKRRSLGGLIFAD
                     LRDVTGLIQIVFDTEVNKEAFEKAENLRSEYVIAVKGKVFERQSKNLEMATGEIEVYA
                     EELRILDTAETPPIYIKDNDDVSEQMRLKYRYLDLRKPSMQKNLLMRSKTSKVIRDFL
                     FENGFNEIETPFLAKPTPEGARDYLVPSRISTGNFYALPQSPQIYKQLLMVSGMNRYF
                     QIVRCFRDEDLRANRQPEFTQVDIEMSFVDAEDVMAVNERLIQRIFKEVKNIDIKLPL
                     RRMPYNEAMDKYGCDKPDLRFGYELKNITGVFKNSEFNAFKSVYESGGLIKCIKIDGA
                     SEDYSKKDISKYEKFVKSHGAKGLAWFKYENGGFDSPLAKFLSQGEINEMISLLEIKE
                     KDIVFIVADNESAVNTALSALRIEIARERNLIDNDVYELVWITEFPLFEYSEEEKRYV
                     AKHHPFTSPVDEDMDKIESAPETVRAKAYDIVINGDEIGGGSIRITNRNLQSRMFKAL
                     GFTEEEAQRKFGYLLEAFKYGVPPHGGIAFGLDRFMMLLTGATSIKDVIAFPKTQSAS
                     CLMTESPSQVSEKQLDELKITLNE"
     assembly_gap    5139..5238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5239..6034)
                     /gene="pdaA"
                     /locus_tag="PKK75_06260"
     CDS             complement(<5239..6034)
                     /gene="pdaA"
                     /locus_tag="PKK75_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HNZ83237.1"
                     /translation="MVLRMNKMLFIIILTGLILLAVIFCLNIIKNLSVTVSTNISNWG
                     LKFNADGTVPMGNASKDFLKKYNAYYVGGPDEKYIYLTFDAGFENGNTANILDVLKKH
                     DVKAAFFLVGHYLEKEPELVKRMVEEGHIVGNHTYHHPDMSLISDKESFARELTSLEE
                     LYKEITGQEMPKYYRPPQGKFNEENLRMADELGYKTIFWSLAYVDWYKDKQPTKEHAF
                     SKLIPRIHPGAVVLLHSTSETNEQILDELLTRWKDLGYTFKTLDDLT"
     gene            6178..6537
                     /locus_tag="PKK75_06265"
     CDS             6178..6537
                     /locus_tag="PKK75_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HNZ83238.1"
                     /translation="MLEYLINLIVPNIAHLLELIGVFIILYSAIKAFIKFGLRHFNFG
                     DENIKIELSQALAMALEFKLGAEILKTLVIRTIDELIILASIVILRAALTFIIHWEIE
                     TSKRGTNHTESNKSNNM"
     gene            6763..7083
                     /locus_tag="PKK75_06270"
     CDS             6763..7083
                     /locus_tag="PKK75_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83239.1"
                     /translation="MNDNIIDQIIQIDEVSYENQEKKKSELLKIKQTYEEAINIYKKE
                     KLNDAKHSAKSITEQMDLILKNEEEKQRIEVGKISEEIDTTYKNSEKMLIEKLLNELF
                     ALEG"
     gene            7085..8113
                     /locus_tag="PKK75_06275"
     CDS             7085..8113
                     /locus_tag="PKK75_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNZ83240.1"
                     /translation="MRSPYDTHYAVKTKITAKRGRIFKKLEWKKILDFTTVDQLTDYL
                     KKSEAFQDVLKDVKNNINRDNLEIILGRFKTLEIEKLLHYYSGAYKEFIESFLAESDL
                     TDISLILRRIARNEDLNDISSRFIHSERFTNIPYDKLLSSNNVDQFVEGLKGTPYYND
                     LRGLTDADAIKRDFHIEMKLYVSYYKSQLEKADKLFMDDKEAVKGIIGTKIDFLNVLW
                     IYRAKNYYHITPAEMLNYSLEGGNELKFHKLKKLCFAENKDDFNEMIGETFGNKFKND
                     LNNIDTSLAMNYFMYDYLSKNVFENLGLVLSYIYMLDIVINNLTAITEGIKYRLPKDN
                     LKNYLVNI"
BASE COUNT         2471 a         1512 c         1260 g         2819 t
ORIGIN      
        1 aaaaaaataa taaaaatatt ttgtgaaata tttttattat tttctgcctt atactatggc
       61 agcagtttat atacaaatga accatttttg ttgatcttgc tgtaaacatt gcttacggga
      121 aatctgcccc tgcattcatt taaaactata tcaagtgtct tttggttatt agtctttata
      181 catatatcac actcattctt tatgctgaaa ggtgtctgca ccagttgaat atttttaacc
      241 ccgtatcctt gcagcctgtt gaatgctgca acagcataat tcccgtgcaa aaaggtaata
      301 atataaacct ccataggata ccccctcacc tatattctat taacatgggc gaagaaggtt
      361 ccatggaggc atcttaagtc ttttaattta tttgtatttc ttttgcttct tcaggtttga
      421 agtcattttt caaatcgtaa atattatgtg atgtgtagtc ccttaattgt ctgtcaccca
      481 atgaataggt cctcatgaac tttttggctg ttactatagt ccaatgctct gcatcccatg
      541 tcaaatatcc atcgtcatcg gtttttttat catatagtat taaatcaatc tgtctgtttg
      601 cttctgtcat taaattaatc aaatcctgtg ttgtaacatc catttctacc ttgtttatta
      661 agtcgtaggc tttcattatt ttctcctcct tttaacagga gcaccgttaa tttatatatt
      721 tatgctcctc gtcattttca taaatattta attcatccct taagaattcc aggtttgact
      781 cataatcctc ttcagaaatt tgattgctga aaagcagcag atccagcctg ttcttttggt
      841 cttcaaacaa gaaaatttcc ttgcatggcc atactgtgtt tttcttattg tcagcctttg
      901 attttaaaat tgttatattg atttcatctt ctttaaggtc tgatatggtc cccatcatat
      961 agaattcatt tattttaatt tgattgcttt caaacaaagt tctgattttt ttgataaacc
     1021 tgtaatggtc ttttttgatt tcaaaagaaa catcagaatt taacatatct tcttccaagt
     1081 ccggattgta gaaatccaca tggacatcgt aagctaaatc cttatttaaa ttttgaaaat
     1141 cgattttatc atttattata ttttcaatat ttgggtcggt aattttacca aaaagtttgt
     1201 acatatcctg cccccttact gtctcaaata ttccaggaat tttttaattt tgctttcata
     1261 gcctgattcc ttaggctcat aaaatatggt ttttatggtt tcaggcatat attgctgttt
     1321 tatataaaac ttttcataat catggggata tttatattct attcccctgt tcagcttttc
     1381 agcacctttg tagtgactat cttttaaatg tgcaggtact tcatgagact ctttttcaac
     1441 tgcctccatt gctttatcga ttgcaattac aacagaattg ctcttgggcg cgcatgcggt
     1501 ataaattgca gcatgggcca gtgttatcct gccttcggga aaacctatta tttctactgc
     1561 cctgaatgca ttgacagcga cctccaaagc cctggggtct gcatttccca catcctctga
     1621 agcgcagatt ataattcttc ttgcaataaa cttaggatcc tcgcctgctt ttatcatctt
     1681 ggcaagccaa taaaccgccg catccgggtc tgaacccctc atgcttttta taaatgcaga
     1741 tattgtatca taatggttgt ctccattttt atcatagtca attttctttc tttgaatgca
     1801 ttcagatgca atatccagat ttatttttat catcccgtca gtttcacagg ttgtttccac
     1861 gccaagctcc aatgcattaa gggcgcttct tatgtctccg tttgcatatt ctgcaatatg
     1921 agttaaagca tcctcgtcaa tttccacttt tctgtttcca tatcctcttt tgtccgccaa
     1981 agattttagt acaacagcct tgatatcctc tatgtcgata cttttgaatt caaatatcaa
     2041 ggaccgtgaa atcaaagcag ggttaacttc aaagtaagga ttttcagtgg ttgctccaat
     2101 caatattatt gtaccatttt ccacgtaagg aagcaaggca tcttgctgag ctttattgaa
     2161 acgatgaatt tcgtctataa aaagaattgt tctcttatta taaatcgaaa gctcgtcaat
     2221 tgcatagctt attttttcct ttatatcctt tataccgctg gtaacagcgc ttaaggttgt
     2281 aaaattttta tttgtggtat ttgcaataat tttagccagg gttgtcttgc ctgttccggg
     2341 aggtccgtaa aaaattgcag aagtaagctt atcagcttct attgccctgt ttaaaatttt
     2401 cccctggcct acaatatgct cttgtcccac aaattcgttt aagtttaaag gtctcatccg
     2461 ctctgcaaga ggaccttctt tttttatgga attttggtat tgcatttcaa aaatattcat
     2521 tttaccacca tttggagaca ttccccgata ggatgaccgg aaaagaatgg tcctcaatca
     2581 aggtgtgtcc tctattcggg catacctaga ctttaaagac tattcctaaa gtcaaggtgc
     2641 gtcccctgtt atagtttctt tccgattgta aatttcaata cattcttttt catttctctc
     2701 ttatcataag ccttcaagag gtaaaatgaa attatcaatg aaaacaatga ggctatagca
     2761 gaaattatat ccttattggg acctgaaata atcatttgga aaataaaaac cgttgccagc
     2821 atcaaaacca aaggaagtcc gtacaaaact gcaatatgct tgagcatcac ttcattttca
     2881 gtattaattt ccacaaaatc gcccacatta atctggtctt ccacatcaga aatatcagtt
     2941 tctaaaatta cactgttaac cttgcaggcg gagctgcagc ttttgcagct gtccccgcag
     3001 gctgataccc ttttaacgga aataacagcc atgtttccat ctattttttc tatccttcct
     3061 acattatcca ttatatttcc ttcctgtaat ttactcattt aacgtaattt tcaattcgtc
     3121 aagctgcttt tcagatacct ggctgggaga ttcagtcatc aggcaggaag cactctgtgt
     3181 ttttggaaat gcaattacat cctttatact ggttgcaccc gttaaaagca tcataaaacg
     3241 gtctaatcca aatgcaattc ctccgtgggg aggcacgccg tatttaaatg cttccaaaag
     3301 ataaccaaat ttcctttgtg cttcttcctc tgtaaatccc agggctttga acattctgct
     3361 ctgtaaattt ctgtttgtta tacgaatgct gccgccgcct atttcatcgc cgtttattac
     3421 tatatcgtat gcttttgctc tcactgtttc cggagccgat tctattttat ccatatcctc
     3481 atctacaggt gatgtaaagg ggtggtgctt agccacatat cttttttctt cttcgctgta
     3541 ttcaaacaag gggaattcag taatccatac caattcataa acatcattgt caattaaatt
     3601 tctttccctg gctatttcga ttcttaaagc gctaagtgca gtattaactg cggattcgtt
     3661 gtcggcaaca ataaatacaa tatctttttc cttgatttcc agcaaggaaa tcatctcatt
     3721 tatttcacct tggcttaaaa atttagcaag aggtgaatca aatccgccgt tttcatattt
     3781 aaaccaggct aaaccttttg caccgtgaga tttaacaaat ttctcatact ttgaaatgtc
     3841 cttcttggaa tagtcctccg atgccccgtc tatctttatg catttaatca aaccgccgct
     3901 ttcatatact gatttaaagg cattaaattc agaattttta aatacacctg taatattttt
     3961 aagctcatat ccaaaacgca aatcaggttt gtcacagcca tatttgtcca tggcttcatt
     4021 ataaggcatc cgtcttaatg gaagcttaat atctatattt tttacttcct tgaatattct
     4081 ttgaataagt ctttcgttta cagccattac atcttctgca tcaacaaaag acatttcaat
     4141 atcaacctga gtgaattcag gctgtctgtt tgcccttaag tcttcgtctc tgaagcatct
     4201 tactatctgg aaatacctgt tcattcccga aaccatcaat agctgcttgt aaatctgagg
     4261 cgactggggc agtgcataaa aattgccggt tgaaattctg ctgggtacca aatagtctct
     4321 tgccccttca ggtgtaggct tcgctaaaaa tggtgtttcg atttcgttaa agccgttttc
     4381 aaataaaaag tctctaataa cctttgatgt tttgcttctc atcaagagat tcttttgcat
     4441 tgaaggcttt ctcaagtcaa gatatctgta ttttaacctc atttgttctg aaacatcatc
     4501 attgtccttt atatagattg gaggagtttc tgcggtatct aaaattctta gctcctctgc
     4561 ataaacttct atttctcccg tagccatttc aaggttttta gactgtcttt caaatacctt
     4621 cccttttaca gcaataacat attcacttct taagttttct gctttttcaa aggcttcctt
     4681 gtttacctcc gtatcaaaaa caatttgaat caaacctgta acatccctta agtcggcaaa
     4741 aataagcccg cccaaacttc ttcttttttg tacccacccc ataagcacaa cttcactgcc
     4801 aatgtggtcc ttgttcagct ctccgcacat atttgttctt ctaagtgttc ccattgtatc
     4861 cataatcata tcctccatat tatactatat tcttgctttc aggctgcacc attattccct
     4921 ttggaacaaa aaactcgtcc cctgtcttag ggacgagtat aacccgcgtt gccaccctat
     4981 ttggacttaa agtccctctc attaaaatct gctttccctg aggtgtcttc atcacaagtt
     5041 gataacaggc ttccatcatc cctgtcttgc tataatcatt gctttatgac tactcttccc
     5101 ctgcaattta tttacactta aattaccaca ccatttttnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnntt gtaaggtcgt ccagggtttt gaatgtgtag cctaagtctt
     5281 tccaccttgt taaaagctca tctaaaattt gctcattggt ttcagaagtg gagtgcagta
     5341 aaaccaccgc ccccggatgt atacggggaa tgagtttgct gaatgcatgt tcttttgtag
     5401 gttgtttgtc tttgtaccag tctacataag ccaggctcca gaatattgtt ttatatccta
     5461 attcatcagc catccttaaa ttttcttcgt tgaatttgcc ttggggaggt ctgtaatatt
     5521 tgggcatttc ctgacctgtt atttctttat atagttcttc cagggaagtc agctccctgg
     5581 caaatgattc cttgtcggaa atcagcgaca tatcgggatg gtggtaggta tggttgccca
     5641 caatatgacc ttcctcaacc atccttttaa caagttcagg ctccttttcc agatagtggc
     5701 ctaccaggaa aaatgcagcc ttcacatcat gtttttttaa tacatcaagt atgttggcag
     5761 tatttccgtt ttcaaatcct gcatcaaatg ttagataaat atatttttca tccggccctc
     5821 ccacatagta agcattgtat ttcttcaaaa aatccttgct tgcattcccc atgggcacag
     5881 ttccatcggc attgaactta agtccccaat ttgatatatt tgttgaaact gtaacagaaa
     5941 gattcttaat aatattaaga caaaaaataa ctgcaagcaa tataagaccc gttaatataa
     6001 ttataaacag catcttgttc atccttaaaa ccatatatct caccccttta tttatttata
     6061 tatataaaca ggcttattat gtcttaaaat aataaatatt tatttggaaa aacattcttt
     6121 gacaaagtat tataaatgca atataatgtg atggacttaa atgaaggagg agaagatgtg
     6181 ttggaatatc ttataaacct tatagttcct aacattgctc atttgcttga acttataggg
     6241 gtatttatta ttttatactc ggcaataaaa gcatttatta aatttggatt gcgtcacttt
     6301 aattttgggg atgaaaatat aaaaattgaa ttgtcccaag cattggcaat ggctttggaa
     6361 tttaaattag gcgccgaaat attaaaaaca ttggttatca gaaccataga tgaactaata
     6421 attcttgcat ccatcgtaat attaagagca gccttgacat ttataataca ttgggaaata
     6481 gaaacatcaa agaggggaac taaccataca gaatcaaata agtctaataa catgtaacat
     6541 taaagtagtt ataaaattct aagattaatg acaagcctgc cattttggcg ggtttccgtt
     6601 tttattatat ttataaactg atataatgtt tatacttgtt atatttgaac ataataaact
     6661 ataataaaac tttttataac ataaattttt atgcttgaat ttaataaatc gcaagcgtat
     6721 aatcatatag ttattttatt accttggata aggagtgata cattgaatga caacattata
     6781 gatcaaataa ttcaaattga tgaagtttcc tatgaaaatc aggaaaagaa aaaatcagag
     6841 ctgttaaaaa taaagcagac ctatgaagaa gcaataaata tttataagaa agaaaaacta
     6901 aatgatgcta aacatagtgc caaatccata actgaacaaa tggatctgat attaaaaaac
     6961 gaagaggaaa aacaaaggat agaagttggc aaaatttctg aagaaataga cacaacatat
     7021 aaaaattcag aaaagatgct tattgaaaaa ttgttaaacg agctttttgc attggagggg
     7081 tagtatgaga agcccttacg atactcatta tgcagtaaaa acaaaaatta ccgctaaaag
     7141 ggggcgtatc tttaaaaaac ttgaatggaa aaaaattttg gattttacta cggttgatca
     7201 attaactgat tatctgaaga aaagtgaagc ttttcaagat gttttaaagg atgtaaaaaa
     7261 taatatcaat agggacaatt tagaaattat attaggcaga tttaaaacct tggaaattga
     7321 aaaattgctt cattattatt caggagccta taaggagttt attgaatcat ttttagcaga
     7381 atcagacttg acagatataa gtttaatact tagaagaatt gcaagaaatg aggatttaaa
     7441 tgatataagc agcagattta tacattccga aagatttaca aacattcctt atgataaatt
     7501 gttatcatca aataatgtgg atcaatttgt agaaggttta aaagggactc cttattataa
     7561 cgacttaagg ggtttgacag atgcagatgc aataaaacgc gatttccata ttgaaatgaa
     7621 attatatgta tcttactata agtctcaatt ggagaaagca gataaactat ttatggatga
     7681 taaagaggct gttaagggta ttattggcac taaaattgat tttttaaatg ttctgtggat
     7741 atatcgcgca aaaaattatt accatattac acctgcagaa atgttgaatt acagcttgga
     7801 aggaggaaat gaactgaaat ttcataagtt aaaaaagctt tgttttgctg aaaataaaga
     7861 tgattttaat gaaatgatag gtgaaacatt tggtaataag tttaaaaacg atttgaacaa
     7921 tatagataca agtttagcca tgaactattt catgtatgac tatctaagta aaaatgtatt
     7981 tgaaaattta ggattagtat tatcatatat ttacatgcta gatatagtaa taaacaacct
     8041 aactgcaatt acagagggaa taaaataccg cctccctaag gacaatttaa agaactattt
     8101 ggtaaatata taggtaattg cgaaactatt tttcattaat gaaaaaacga aaaacaaact
     8161 tg
//
LOCUS       DAOPUT010000066         8089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_8890_length_8089_cov_18.638785, whole genome shotgun sequence.
ACCESSION   DAOPUT010000066 DAOPUT010000000
VERSION     DAOPUT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8890_length_8089_cov_18.638785"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="dprA"
                     /locus_tag="PKK75_06280"
     CDS             complement(<1..1018)
                     /gene="dprA"
                     /locus_tag="PKK75_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNZ83241.1"
                     /translation="MNNLNINQKTLLWLSSGGISNGRITKVLDCFGSPKDLWDNFEIE
                     KHNLSMLKTETINELSESKNFFEEKLLEKLNVEKARIVTIFDDEYPSKLQQIEGAPNL
                     LYYKGSLNDINNISIAVIGSRKATTYGKWAAEKLVKELSEIGVNIISGLAVGIDTIAH
                     KTALKYKASTYGIIGCGINVVYPKKNLELFHQISESNGAVITEYPFDIQPVAFNFHER
                     NRIISGLAEGVLVIEAQEKSGTLITAGHAANQGREIFAVPGNIDSLYSRGTNALIRDG
                     AKITMSIDDIVEEIMELKEKVRLKNTSLDLSTFTDDEVSIIGCLETGENTIDEISRAT
                     KMETG"
     gene            complement(1020..2546)
                     /locus_tag="PKK75_06285"
     CDS             complement(1020..2546)
                     /locus_tag="PKK75_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNZ83242.1"
                     /translation="MLSKIKTCVLYGLEGYEVDVETDLSGGLPNFNIVGLPDISIKES
                     KERVRSAIKNSRFDFPVSRITVNLAPANLKKEGSQIDLPIAAGILAASKAIYKPVDEK
                     TCIIGELSLDGRITAIDGALPMVISMRNKTFKKVIVPEDNKEECGAIEGIDIIPVGSI
                     SQLVSYLNEELAIDPFIYKYDFSLKQDYYSEDFSEIKGQPAMKRAVEIAAAGMHNILL
                     LGSPGSGKTMIAKRIPTILPDLTFEESLEVTKIYSICGLLDKKGLMAKRPFRSPHHTS
                     SRTSMAGGGSKPRPGEVSLSHYGVLFLDEIPEFPKNVIEVLRQPMEDGKISISRASGS
                     FTFPAKFMMIASMNPCPCGYLGDPNHECSCSQGQIDKYLGKISGPLLNRIDIQIEVLP
                     VKYNELKDERVEETSEEIKKRIVKARNIQRERYKDMGILTNSELSTKAVSKFCKVNKE
                     SEVLLKNAFELLGLSVRAYNKILKVARTIADLENYENIETKHIAEAIQYRNLDRKYWR
                     "
     gene            complement(2688..3329)
                     /locus_tag="PKK75_06290"
     CDS             complement(2688..3329)
                     /locus_tag="PKK75_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNZ83243.1"
                     /translation="MLSKIKNNCLGKFPGGIIVLYNKGFVIMNKEEYSEYRDSSKLSN
                     QEEDMYKEELAIISQAVLNEMEGYEFYKMAGEQAKTQGNKEAFAHLAKEEIKHAEYLK
                     KLWTILSDGGEIKIKEIMSSGIDIPSPEIYRWDKVDKAYATLGMSIFGIGMQMEKSSI
                     DFYEDAKQKSGSQPVKDLFDLLIKWENVHLDQFTNQYNMLKEEWWAEQQFAPF"
     gene            complement(3538..4284)
                     /locus_tag="PKK75_06295"
     CDS             complement(3538..4284)
                     /locus_tag="PKK75_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HNZ83244.1"
                     /translation="MKVFSVIGVTKSGKTTTIENIISELRRRGYTVGSVKEIHFKKFK
                     MDVEGTNTHRHKIAGSQLVTARGAYETDIMFQEKLDINDLLNFYNHDYVVMEGVRDTC
                     APKIVTAYDVEGILDRMDETTFLISGQISNGMREFEGIPVINSITEIERLVDLIEEKV
                     FDRLPDMKDECCRKCGYTCSQLSSKILRGQKEMGDCALSKQDVILKINGKEIIMVPFV
                     QNILKNAVEAVSKELSGYTESGDIEVCIKR"
     gene            complement(4471..5178)
                     /locus_tag="PKK75_06300"
     CDS             complement(4471..5178)
                     /locus_tag="PKK75_06300"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HNZ83245.1"
                     /translation="MNSELKEIISKSSNIVFFGGAGVSTESGIPDFRSSDGLYSAKYK
                     HSPEVMVSHSFFKTNTEEFFDFYKNKMIFPDAMPNAAHKKLAELEKEGKVKAVITQNI
                     DGLHQKAGSRNVLELHGSVHRNYCMKCGKFHDVNFIIKSKGIPKCLCGGIVKPDVVLY
                     EESLDSEIIEKSVHFIEHADVLIVGGTSLVVYPAAGLINYFKGSKLVLINKSSTSMDR
                     RADLVINDSIGKVFSSV"
     gene            complement(5335..6429)
                     /locus_tag="PKK75_06305"
     CDS             complement(5335..6429)
                     /locus_tag="PKK75_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ83246.1"
                     /translation="MKKGIILIALLLMTSCAAPAADTSFDTPPEADEPVLEEPSDEEH
                     DKVTENEDSKELGEEDIIASIDMSHKPNELGEIMILMYHGIGPEESLWQRTPDNFRKD
                     LEYMYQNGYRMISLNDYARGEIKIEAGFTPVILTFDDGRRNNFNLIEEKGEMVLDPDC
                     AIGILEEFKNKYPDYNVTASFFLGTNTFGQKEYTEQKLNWLINNGYDLGNHTYSHKDL
                     GKLNSLEIQIEIGFINNILKQYLPDYDVETLALPQGSNPRDEFMESVRQGEYQGNKYR
                     TIAVLDVGWRPAYSPFDTLTDFNSLYRVTASEINVDDCGLYDYLKQYEDNKRERFISD
                     GNPDVVTIPKRHIDYLNMDIVGDRIVNIYE"
     gene            6627..7148
                     /locus_tag="PKK75_06310"
     CDS             6627..7148
                     /locus_tag="PKK75_06310"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HNZ83247.1"
                     /translation="MKSDNRNAQDKLKLLYILDFIDLPLTNVEITNYILEYGMMDYFS
                     LQLLLSDLCDAKFTTIKPYNGNEYYSVSKEGRDALEMFKNKLPEYFINEVKTNFPQIK
                     KQIEKQREIFGHYFKRKDDEYIVSLQVTENSATIFNLSINVTDEITARNIIKKWKNEP
                     EEIFGKIINALTN"
     gene            complement(7145..8035)
                     /locus_tag="PKK75_06315"
     CDS             complement(7145..8035)
                     /locus_tag="PKK75_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83248.1"
                     /translation="MYDEKIVTTGISQGKLFMFIIDLSGEDYSFKKAFYLGAGDKFNL
                     HSIEYFHNAVYMADSYNNKIYKFDNNNFFETNVGRDPRHMCFDRENLYVANFESDNIS
                     IIDLSSFTLTGSIPAGIKAHDVIYSEKNKCLYTSCYEENEVVEYSIDKGIRRRFITDG
                     KPMHLYVLKDTIIVMTYFVNGEIQTKINFINLINKNVEDTININGLASDFLLDYENSI
                     LYILNIDDKNLYIADAMLRNIINKIYLGGYPESISSGSKNIYVTNSKKQQIDIIEKKR
                     HFLSSSLKLSFTPNLIKAIL"
BASE COUNT         2254 a         1669 c         1317 g         2849 t
ORIGIN      
        1 gaccggtttc catttttgtt gcccggctta tttcatcaat tgtgttttcc ccggtttcaa
       61 ggcagcctat tattgatact tcgtcgtcag taaatgtgct taaatcaaga gatgtatttt
      121 taagtctgac tttttctttt aattccatga tttcttccac gatatcatca atagacatgg
      181 ttattttagc tccgtctctt atgagggcat ttgttcccct gctgtagagg ctgtcaatgt
      241 ttccgggaac cgcaaaaatt tctcttcctt gattggctgc gtgtcctgct gttatcaatg
      301 tgccgctttt ttcctgggct tcaataacca ggactccctc tgcaagaccg ctaataattc
      361 tatttctttc atgaaaatta aatgcaacag gctgtatgtc aaaaggatac tctgtaatta
      421 cagctccatt gctttctgat atctgatgaa aaagctccag gtttttcttt ggatatacaa
      481 catttatgcc gcagcctatt ataccgtagg ttgacgcctt gtattttaaa gctgttttat
      541 gagcaattgt atcgattccg actgccagac cgctgattat atttacaccg atttccgata
      601 gttcttttac cagcttttct gcagcccatt taccgtaggt cgtagccttt cttgacccta
      661 tgactgcaat tgatatgttg tttatgtcat ttaaagagcc cttgtagtag agcaaatttg
      721 gagcaccttc aatctgctgc agcttggatg gatactcatc atcaaaaata gtaactatcc
      781 ttgccttttc tacatttaat ttctccagca atttctcttc aaaaaaattt ttgctttcag
      841 aaagctcgtt tattgtttct gtcttaagca tggataaatt atgtttttct atttcaaagt
      901 tatcccatag gtccttcggt gatccgaaac aatcaaggac ttttgttatc cttccgtttg
      961 aaattccccc cgatgacagc cacagtaaag tcttttgatt gatatttaaa ttgttcatgc
     1021 tacctccagt atttccggtc taaatttctg tactgaattg cttcagctat atgctttgtt
     1081 tcaatatttt cgtaattttc caaatccgct attgttcttg caacctttag tattttattg
     1141 tatgccctga cacttaagcc taaaagttca aatgcattct taagcaatac ttcagattcc
     1201 ttattaacct tacagaattt tgatacagcc tttgttgaaa gctctgaatt agtcaggata
     1261 cccatatcct tgtatctttc cctctggata tttcttgctt ttacaatccg ttttttaatt
     1321 tcctctgaag tttcttccac tctttcatcc ttcagctcat tatatttaac cggcagtact
     1381 tctatttgaa tatcaatccg gttaagcaga ggacctgaga ttttccccag gtatttgtca
     1441 atctgccctt ggctgcagga gcactcatgg tttgggtccc ccaggtatcc gcatgggcag
     1501 gggttcattg aagcaatcat cataaacttt gcaggaaatg taaaggagcc gctggctctt
     1561 gatatggaaa ttttaccgtc ttccataggc tgacggagaa cttcgataac atttttagga
     1621 aattcgggaa tttcatctaa aaataaaact ccgtagtgag aaagggatac ttctccggga
     1681 cggggttttg accctccccc tgccatggag gtgcgggagg aagtatggtg gggtgaacgg
     1741 aaaggtcttt ttgccatcaa acccttcttg tccaaaagac cgcatatact gtagattttt
     1801 gtaacttcca gggattcttc gaatgtcagg tccggcagga tagtcggtat tctttttgca
     1861 atcattgttt tgccggaacc gggacttcct aaaaggagaa tgttgtgcat ccccgctgct
     1921 gctatttcaa ctgccctttt catggcaggc tgtcccttta tttctgaaaa atcttcactg
     1981 taataatctt gtttgagaga aaaatcatac ttgtatataa aggggtctat ggccaattct
     2041 tcgtttaaat aacttactaa ctggcttatg cttccgacag gaattatatc tatgccttca
     2101 atggcgccgc attcttcctt attgtcttcc ggcacaatta ctttcttaaa agtcttgttc
     2161 ctcatggaaa taaccatggg gagggccccg tcaattgcgg ttatccttcc gtccagagat
     2221 aattcaccga taatgcatgt tttttcatcc acaggcttat atattgcctt ggaagcagca
     2281 agtataccgg ctgcaatagg aaggtctatc tggcttcctt cctttttcaa atttgccggt
     2341 gcaagattta cggtaatgcg gctgacagga aaatcaaatc tgctgttttt aatagcagac
     2401 ctaactcttt cctttgactc tttaattgaa atatcaggca ggcctacaat attaaaatta
     2461 gggagacctc ctgataaatc tgtttccaca tcaacttcat aaccttcaag tccgtaaagg
     2521 acacatgttt taattttgga aagcatatag cccctccaat aattcaggtt ataaaattat
     2581 actataattt tccattttat acaatcgata ataaggcttt aaggcctttg aatttacaac
     2641 aaaaatctcc ccttttcgca aaacgcaaag gggagatttt tcatcattta aaatggtgcg
     2701 aattgttgtt ctgcccacca ttcttccttc aacatattgt attgattggt gaattggtct
     2761 aagtgaacat tttcccattt aatcaacaag tcgaataagt cttttactgg ctgggagcct
     2821 gatttttgtt tagcatcctc gtaaaaatct atggagctct tctccatttg cattcctatg
     2881 ccgaatatgg acattcccag tgttgcatat gctttatcga ccttgtccca tcggtaaatt
     2941 tccggcgaag gtatatcaat tcccgaagac attatttctt taatttttat ttctcctccg
     3001 tcactgagta tggtccataa tttctttaag tattctgcat gctttatttc ttcctttgcc
     3061 agatgtgcga atgcttcctt gtttccctgt gtttttgcct gttcacctgc catcttatag
     3121 aactcgtagc cttccatttc atttaaaaca gcctgggaaa taatcgccag ctcttcttta
     3181 tacatatctt cctcctggtt tgataatttt gaagaatccc tgtactcaga gtactcttct
     3241 ttgttcatga taacaaatcc tttattgtat aatactatta taccacccgg aaactttcct
     3301 aaacaattat tctttatttt cgagagcatt ctttactgca gcacttcttc ttttttcaat
     3361 ggctttcaac tctttttttt cttctgcaat tattttttct ctttcaatac ctaactcttc
     3421 agacaatatt tcaatgaatt tcccttatat aatcctcacg attttcaaat ttttttaaaa
     3481 tatatgtgcc ttcatggtca acaattcgaa acacatggtt tctcttgctt gttagtttta
     3541 tctttttatg catacttcaa tatctccgct ttcagtgtag ccggataatt cctttgaaac
     3601 tgcttccacg gcatttttta aaatattctg aacaaaggga accatgataa tttccttgcc
     3661 gtttattttt agaattacat cttgctttga caaagcgcag tcacccattt ctttttggcc
     3721 tcttaaaatt ttagagctta actggctgca tgtgtaaccg cacttcctgc agcattcatc
     3781 cttcatatcg ggaagtctgt caaacacttt ttcttctatc agatcaacca gtctttctat
     3841 ttcagttatt gaattgataa cgggaattcc ttcaaattct ctcatgccat tggatatttg
     3901 tccggatatg agaaaggttg tttcatccat cctgtctaaa attccttcca catcataagc
     3961 tgttactatt tttggagcac atgtatccct gacgccttcc attacaacat aatcgtgatt
     4021 gtaaaaattc aataaatcat ttatgtctaa tttttcttga aacattatat ccgtttcata
     4081 agcacctctt gcggttacca gctgtgagcc tgctattttg tgcctgtggg tgttggttcc
     4141 ttccacatcc attttaaatt tcttaaaatg tatttctttt acgcttccaa cagtgtaacc
     4201 acgtcttcga agctcactta ttatgttttc tatggtggtg gttttgccgg actttgtaac
     4261 ccctattact gaaaaaacct tcatcttaag aatccttcct catctttatt gtaaaatatc
     4321 atatccttcg acgcggctcg tttactttgc tttcatcagc tggtcacttc cactgatccg
     4381 tgttgccgtt accatatccg tcattttatt ctcgaatatg ggtaaaaagc agattgcaga
     4441 gaaaccatga aagctattaa ataggaacat ttatactgaa ctaaaaacct tgccaatgct
     4501 gtcattaata actaaatcag cccgtctgtc catagatgtg gatgacttgt taatcagcac
     4561 aagtttgctg cccttaaaat aattaataag acctgccgca gggtagacaa caagagatgt
     4621 cccccctaca attaaaacat ctgcgtgttc gataaaatga acagactttt caataatttc
     4681 gctgtcaaga ctttcctcgt acaacacaac atccggtttt acaattccac cgcacaggca
     4741 ttttggaatg ccctttgatt ttataataaa attcacgtca tggaatttcc cgcatttcat
     4801 acagtaattt ctgtgtactg acccatgaag ctccaataca tttctgcttc cggctttctg
     4861 gtgaagaccg tcaatattct gggttataac agcttttacc tttccttcct tttcaagttc
     4921 tgcaagtttc ttatgggctg cattgggcat ggcatcggga aaaatcattt tatttttgta
     4981 gaagtcaaag aactcttcag tgttagtctt gaaaaaactg tgagaaacca tcacttcagg
     5041 cgaatgctta tattttgcgc tatataatcc gtctgagctt ctaaaatcag gtatgccgct
     5101 ttctgttgaa acacctgctc ctccaaaaaa tacaatatta ctgcttttcg aaattatttc
     5161 ttttagttca gagttcacaa caacctcccg tttattatat tcttaaatca ataataccac
     5221 attcccctgg ttttctcaaa ctaatcgcat aaaaagggag cagttcatgg ctgccccgct
     5281 gatttcatcc gttgtaagca aaacatctta tattacaagc agatttcttt ttgtttattc
     5341 gtaaatattc actatcctgt cccccacaat atccatgtta agataatcga tatgcctttt
     5401 aggaatggtt accacgtcag gattaccgtc gctaatgaat ctttcccttt tattatcctc
     5461 atactgtttt aaataatcat ataaaccgca atcatccaca ttaatttcgc ttgcggtcac
     5521 ccggtaaagg ctgttgaaat ctgtcaaggt gtcaaagggt gaataggcag gcctccagcc
     5581 tacgtccaaa acagcaatgg tccggtattt attgccttga tattctcctt gacgcacgga
     5641 ttccataaac tcatccctcg ggttgctgcc ttggggaagc gccaaggttt caacatcata
     5701 atcaggcaga tattgcttta gtatattgtt tataaatcct atttcaattt gaatttccag
     5761 tgagtttaat tttcccaaat ccttgtggct gtatgtgtgg tttcccaaat catagccatt
     5821 gtttataagc caattaagct tttgttctgt atattctttt tggccgaaag tgttagtgcc
     5881 taaaaaaaag ctcgctgtca cgttataatc aggatattta tttttaaact cttctaaaat
     5941 tccgatggcg caatcaggat ccagcaccat ttcccctttt tcttcaatta aattaaaatt
     6001 attccgcctc ccatcatcaa aggtcagaat tacgggagta aatcctgctt ctatcttaat
     6061 ttctcccctg gcataatcat ttaagctgat catgcggtaa ccgttttgat acatgtattc
     6121 caggtctttg cgaaaattat ccggagttct ttgccataat gattcttccg gccctattcc
     6181 atgatacatc aatatcataa tttctcccag ttcattaggc ttatgagaca tatcgatgga
     6241 agcaataata tcttcttctc caagctcttt tgaatcttca ttttctgtga ccttatcatg
     6301 ttcttcatct gaaggctctt ccaaaaccgg ttcatctgct tcaggggggg tatcaaaaga
     6361 tgtatcagct gcaggggctg cacagcttgt cattagtaac aatgcaatca atataattcc
     6421 ttttttcatt tatacactcc tatctgctct tatattctat attattcaat agtaaatgtc
     6481 tatatgtagt attttcaaat ttttgtttat tatcagcaca aactttgata taatgaataa
     6541 atgaacttag gctgccccct aaaagatgaa caaaactaaa atggaaggtg aagacgggaa
     6601 aatcttaaca atataggagg aaaaaattga aaagcgacaa tagaaatgca caggataaac
     6661 tgaagctttt atacatattg gactttatag acctgccttt gacaaatgtt gaaattacca
     6721 attacatact tgaatacggc atgatggact atttctcgct gcagcttttg ttaagtgacc
     6781 tgtgcgatgc taaatttaca accatcaagc cttataacgg aaatgaatac tattcggtct
     6841 caaaagaagg cagggatgcg ctggaaatgt tcaagaataa actgcctgaa tactttatta
     6901 atgaagtaaa gacgaatttt ccccaaataa aaaaacaaat agaaaaacaa agagaaatat
     6961 tcggtcatta tttcaagaga aaagatgatg aatacattgt atcccttcaa gttacggaaa
     7021 attccgcaac catttttaac ctgagcataa atgttactga tgaaattacg gctagaaaca
     7081 taattaaaaa atggaaaaat gaaccggagg aaatattcgg aaaaatcata aatgcactga
     7141 caaattagag aattgctttt attaaattcg gagtaaagct tagttttaat gaagagctta
     7201 aaaagtgcct ctttttttct attatatcaa tttgctgctt tttggaattt gtaacataaa
     7261 tatttttact gcctgaagaa atggattcag gatatcctcc caggtatatt ttgttaatga
     7321 tatttcttaa catggcatct gctatataaa ggtttttatc atcaatattc aatatgtaga
     7381 ggatgctgtt ttcataatca agcaggaaat cagaagcaag cccgtttatg tttattgtat
     7441 cttcgacatt tttattaatt aaatttataa aatttatttt tgtttgaatt tcaccgttca
     7501 caaaataagt cataacaata attgtgtcct ttaacacata taggtgcatg ggtttgccgt
     7561 ctgttataaa tcttcttcta atccctttgt caatactgta ttctaccact tcattctctt
     7621 cgtagcagga tgtgtaaaga cacttgtttt tttctgaata aatgacgtca tgagctttta
     7681 tgccggcagg tatggagcct gtcaatgtga aagaggacag gtcgataatg gaaatattgt
     7741 ccgattcaaa atttgccaca tacagatttt ctctgtcaaa gcacatatgc cgggggtctc
     7801 ttccaacatt tgtttcaaag aaattattgt tgtcgaattt gtatattttg ttattgtagg
     7861 aatcagccat atagactgca ttatgaaaat attctatgct gtgaaggtta aatttgtccc
     7921 ctgcccctaa ataaaaagct tttttaaaag aataatcttc ccctgacagg tctatgataa
     7981 acataaataa ttttccctga cttatacctg ttgtgacaat tttttcatca tacactccaa
     8041 tcacctccat atagtttatg aaatttatca taataagtga ttaaatcac
//
LOCUS       DAOPUT010000067         8014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_9007_length_8014_cov_19.301922, whole genome shotgun sequence.
ACCESSION   DAOPUT010000067 DAOPUT010000000
VERSION     DAOPUT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9007_length_8014_cov_19.301922"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2472
                     /locus_tag="PKK75_06320"
     CDS             <1..2472
                     /locus_tag="PKK75_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNZ83249.1"
                     /translation="RRWGMAVKSDNSRLFKKIARIIVDKRNLLFVLYAFAFIFCIFSI
                     GWAEVENDIVNYLPEDGKTRQGILIMDEEFTTLGRAYIMISNISYSHAKKLADSISEI
                     DGVSTLMFENTEDYYKNSSAKFFLLFDGEEDSEDTQNTLDEIKDMIEPYDTSISTTIG
                     FKYADMLSNDMIIISILALIIISGVLLFTTGAYAGIIVFYINFIAAAALNMGTNFLYG
                     KISFVSNSVAAILQLALAIDYSIIMSHRFSEEREYLPPVEAAIGALSKAIPEIASSSL
                     TTVGGLAAMAFINFGIGFDLSIVMIKGILFSMLTVFTLMPGLLVVFSRLIDKTKHRNF
                     VPSVKKLGRFAIRTRYIMPPVFAVLVVVSFIFSSKCPYLFSMEDVKSYMVSEEQKEKD
                     RINETFGKINQMALVVPSGDYEAERQLLSRLERFDEVDSVVGLSNIKAMGGYTLTDEI
                     TPRQFAELVDLDYEIAQLLYSAYSINDEDYGKVISGIGNYGVPFIDMFEFLYNRVLQG
                     YVSIDDDMMNELDSYYHQLSNTRIQMEGDNYSRFEINSRLPVESRETFDFIRKVYDEA
                     GKFYDNEDVYVMGESSNALELSSTFEKDNIIISVLSVAFVIIVLIFTFQSVGLSILLI
                     AVIQTSIWTNFSFPYLRGHGLYFLGFLLVSAIQMGANIDYAIVITSRYLELKKYMERE
                     NAVVEALNSGFTTVVTSGSILAIAGFLIGFLSTDGTIAILGRYIGQGTIISTILVIFV
                     LPQLLYLGDTIIERTTFILNLPVSVDRPKKKLHIEGRLHGYVKGEIDAFVEGTISGEI
                     QPVRDDSEEPAECAPDRKDVDYEES"
     gene            2459..5797
                     /locus_tag="PKK75_06325"
     CDS             2459..5797
                     /locus_tag="PKK75_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HNZ83250.1"
                     /translation="MKKAESFLILILVFIMILSSAVPASADNLISQTIRIYSADDLKE
                     LSRNCSFDRWSQGKTVILEADIDLRNEAFTPIPTFGGNFDGKGHTISGLYIETEGSNQ
                     GLFRYLQEVGTIKNLKIKGTLAPKGDKSNIGAIVGHNSGVVENCSADAYIKGRDIVGG
                     LAGWNGTSGRIINSSFSGTVYGESKAGGITGYNAGTVLRCTNESSVNTTLEESELDFQ
                     NMTIDDINLERLISDAMDTGGIAGINTGIVMNSVNRGTIGYPNAGYNVGGIAGRQSGY
                     IDDCVNYGTVYGRKEVGGIAGQMDPYVDTIVSPSKLGLLQREMNVLQYSITKMINNTR
                     SASNLMTQNFSHIQGNLDDSMIHIQSLADQTENLINKDIEGINAISVTALESLGRLIP
                     LIESLENTMDIMKDGIDPMERSIRYLFLAMNEMTDIIDELNELSDIPEDIRTEMKKAA
                     ESIESAIGVLQDLLVKIIKVISQDEVLKETFAGLKEELQKDMPDMDKVLEYLNIIHER
                     LQEISVELKEGEISEKLASAHAYMIEAQKNIKKVRDEIKSIGGHIQEIFSSIDDALSY
                     MESALDYMLDAMNIMERAQDDIDDIFDGIRELLEYLKAQPEFEFQTTDELYQKTKDEL
                     FTSIGSMSDSLSSFMDDLSLQSNTMTDDMQSVSDQLFKVMNLVISIFTDLSFNEEDLE
                     DIVQDVSGEDIDKTTEGKVSGSRNLGNVEGDINVGGIAGAMSIEWMLDPEDDLDFKEN
                     LTANTVFQTRVIVDGCTNEGSIVSKKNNAGGIVGSMDLGYIRNCLGAGSIESTAGNYV
                     GGIAGISKGPVVSSYAKCSLRGGNYIGGISGFGKEIIDSYTLVKVNESRACVGAIAGD
                     IDKGSNIKGNYFVSDSIHGIDGISYAEKAEPIDYDILISKENIPSSFKEFKLTFRAED
                     KLIKTIDFNYGESITINDLPEVPLKEGYYGKWEDFDMSNLTFDDEIQAMYIHHVTVLE
                     SNEKRDEGLPVILVEGKFTEEDFITLTKNEVSPDDEGILEQWRIAIPDDGENTHIIRY
                     YPMEETNKPLVYIFSSGSWNMADARQDGKYLVFAGEGNDITFRVIGGNFYYEWIWAGI
                     AAMVLIVTTAWVKRRLKKKASAEG"
     gene            complement(5794..6693)
                     /locus_tag="PKK75_06330"
     CDS             complement(5794..6693)
                     /locus_tag="PKK75_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ83251.1"
                     /translation="MMTKEIKSVFILLLTAAIWGLAFVAQRMGMEYVGAFTFNGIRFA
                     LGSVSLLPVIYFFDKSSKAENSSAEKVDADLKTTIKSGAIAGCILFIAASLQQVGLIY
                     TTAAKAGFITSLYIVLVPILGIFLKQKTYPTTWIGAVTAAVGLYFISINEDFTIQFGD
                     LLQLIGAFFWAAHIQVIDKFVKNVNAIKLSSVQFAVCSVLSIVTAIIFEDISISGIYD
                     ALVPILYGGIMSVGVAYTLQAVGQRHAKPSHAAIALSMEAVFAAIGGILILNETMPMR
                     GYLGSALMLAGMLITQTENIQKS"
     gene            complement(6742..7308)
                     /locus_tag="PKK75_06335"
     CDS             complement(6742..7308)
                     /locus_tag="PKK75_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ83252.1"
                     /translation="MYSDINYIIQESLKGDKIYIEKLLKRLNPLIYKNIYKYNAPSDP
                     IVEDLLQEGYIVILKSLKNYDIDRNVHFLQYVKTNLYYYYINYYKKDIKQRTLSLEYL
                     NETGKTLKSTLVDPAALIIREEEKNELHKCIKGLTPGEKKIINLFYFQGFSIQDISEE
                     LDLKYRSVINMKCNAIKKLRKMIKLMFK"
     gene            7454..7921
                     /locus_tag="PKK75_06340"
     CDS             7454..7921
                     /locus_tag="PKK75_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83253.1"
                     /translation="MKNDIKFNEIEAMLPVYVKDKGNCTEIITKAASFIKTATIETCT
                     KKMADYYNLSMYHNRINYGQELGITNKVPLVINENLIYIYINVREPMFAHDAAFGLFD
                     ANSIDSVYEEDGKAVIELKSGKMIKTRQSVKSVKKGMLDARIAKDIYKEKHKI"
BASE COUNT         2596 a         1352 c         1733 g         2333 t
ORIGIN      
        1 cgaagatggg ggatggctgt gaaaagcgac aactcgagat tgtttaaaaa aattgcaaga
       61 ataatagtgg acaagcgtaa tttattattt gtactttatg cttttgcttt tattttttgt
      121 attttttcca taggatgggc tgaagtagaa aacgatatag taaattattt gcctgaggat
      181 ggcaaaacca gacagggtat tcttataatg gatgaagaat tcacaacctt gggcagagct
      241 tatataatga tttcaaacat ttcatattcc catgcaaaaa agctggcgga ttccatcagt
      301 gagattgacg gtgtatctac tttaatgttt gagaatacag aggattatta taagaactca
      361 tctgctaaat ttttcctttt atttgacggg gaggaggatt ccgaggatac ccaaaatacg
      421 cttgatgaaa taaaagacat gattgaaccc tatgacactt ccatttcaac cacaatagga
      481 tttaaatatg ccgatatgct tagcaatgat atgattataa tcagtattct ggctctaatt
      541 atcataagcg gggtgctttt gtttacaacc ggggcttatg cagggataat agtgttttat
      601 ataaatttta tagcggcagc tgcattaaat atggggacca actttttgta cggaaaaatt
      661 tcctttgtgt ctaattcggt ggctgcaatt ttgcagcttg cccttgccat tgattattcc
      721 ataattatga gccacagatt tagtgaagaa cgcgaatatc ttcctcccgt ggaagccgca
      781 ataggcgctc tttccaaggc catacccgaa attgcttcaa gtagtcttac taccgtgggc
      841 gggttggcgg ctatggcatt catcaatttc ggaataggat ttgatttatc gatagttatg
      901 ataaagggaa tcctattcag catgctgacg gtatttactt taatgccagg attactggtg
      961 gtgttcagca ggttaataga taagactaaa catagaaact ttgttccttc agttaaaaag
     1021 ctgggacggt ttgctatcag aaccagatat attatgccgc ctgtattcgc agtattggtg
     1081 gtggtatcct ttatcttttc cagcaaatgt ccttatctat tcagcatgga ggatgtaaag
     1141 tcctatatgg tcagcgaaga acagaaggaa aaagacagga tcaatgagac tttcggtaaa
     1201 atcaaccaga tggcgcttgt tgtaccgtca ggggattatg aggctgaaag gcagctcctg
     1261 tccagacttg aaagatttga tgaagtggac tccgttgttg ggctttccaa tattaaagca
     1321 atgggcggct atacccttac cgatgagata acacccagac agtttgcaga gcttgtggat
     1381 ttggattatg aaattgcaca gctgctttat tctgcctatt caataaatga tgaagactat
     1441 ggcaaggtta taagcggcat tggaaactat ggcgtgcctt ttatagatat gtttgaattt
     1501 ttatataatc gagtgctaca gggttatgtc tcaatcgatg atgacatgat gaatgaattg
     1561 gattcttatt atcatcaatt atccaatacc cgaatacaga tggaaggaga taattattcc
     1621 aggtttgaga taaattccag actgcctgtt gaaagccggg agacatttga ttttatccgc
     1681 aaggtctatg atgaagccgg aaaattctat gataatgaag atgtttacgt tatgggcgaa
     1741 tcttcaaacg ccctggagct gtcatcaacc tttgaaaagg acaatattat tatcagtgtt
     1801 ctgtcggttg ctttcgtaat aattgtttta atatttacct tccagtcggt aggtttatcc
     1861 attttactta tagctgttat tcaaacctct atatggacta atttttcatt cccgtatctg
     1921 aggggacacg gactgtattt tctaggtttt ttattggttt ccgccataca gatgggagcc
     1981 aacatagatt atgcaatagt aattacaagc cggtatctgg aactgaaaaa atacatggaa
     2041 cgtgagaatg ctgttgttga agccttgaat tcaggattta ccacagtagt aacctcgggc
     2101 agcatacttg ccatagccgg ttttcttata ggatttctct ccacagacgg aaccatagcc
     2161 atattgggaa gatatatagg acagggcacc attatttcta caattcttgt aatatttgtt
     2221 cttccccagc ttctttacct gggtgatacc atcatagagc gcacaacttt catattgaat
     2281 ttacctgtca gtgtggacag accaaagaaa aagctccata ttgagggcag gctccatgga
     2341 tatgtaaaag gagagattga tgcttttgtt gaagggacta tcagcggtga aatccagccg
     2401 gtcagggatg attcggaaga gcctgctgaa tgcgctccgg atagaaagga tgttgattat
     2461 gaagaaagct gaatcttttc taatattaat actggtcttt ataatgatat tatcttccgc
     2521 agtaccggcg tcagccgaca atctgatttc acagacaatc cgtatataca gcgctgatga
     2581 tttaaaggaa ttatcaagga actgttcttt cgacagatgg tctcaaggga agactgtaat
     2641 tttggaagca gatattgacc tgaggaatga agcatttacg ccaataccta cattcggcgg
     2701 taattttgac gggaagggac ataccataag cggcttatat atagaaacgg aaggttcaaa
     2761 ccaagggctt ttcagatatc tgcaagaggt cgggacaatt aaaaatttaa aaataaaagg
     2821 aaccttggct cctaagggtg acaaaagcaa tattggtgca attgtgggtc ataatagcgg
     2881 agtggtggaa aactgcagtg ccgatgctta tattaagggc agggatattg taggaggact
     2941 tgcaggatgg aacggaactt caggcaggat aataaattca tccttcagcg gaacagtata
     3001 cggcgagagc aaggcaggcg gcataacggg gtataatgcc ggcacggttc tcaggtgtac
     3061 taacgagtcc agtgtcaata ccacactgga agaaagcgag cttgattttc agaatatgac
     3121 catagatgat ataaatcttg agagactcat atccgacgct atggatacag gaggaatagc
     3181 aggaatcaat acgggcattg tgatgaattc cgtaaacaga ggtactatag gttatcccaa
     3241 cgcaggatac aacgtgggag ggattgcagg gaggcaatcg ggatatattg atgactgtgt
     3301 gaattacggc actgtatacg ggcgaaaaga agtgggcggc atagcgggcc agatggaccc
     3361 ctatgtggat acaattgttt caccgtcaaa attgggactc ttgcagaggg aaatgaatgt
     3421 tttgcagtat tcaattacaa agatgatcaa caataccagg tcagcttcca atttgatgac
     3481 acagaatttt tctcatattc aaggaaacct tgatgacagc atgattcaca tccagtcact
     3541 tgccgaccag actgaaaatt tgattaataa agatattgaa gggattaatg caataagcgt
     3601 aacagctctt gaatctttag gccggctgat tcctttaata gaatcacttg aaaacactat
     3661 ggacattatg aaagacggca tagacccgat ggaaaggtcc ataagatacc ttttcttggc
     3721 aatgaatgaa atgacggata ttatagatga gcttaatgaa ctttcggata taccggaaga
     3781 tatcagaact gaaatgaaaa aagcagcaga aagtattgaa tcagctatag gtgtgttaca
     3841 ggatttattg gtaaaaatta ttaaggtaat aagccaggat gaagtactga aagaaacttt
     3901 tgcgggtcta aaagaagagc tgcaaaagga tatgccggat atggataagg tattggaata
     3961 tttaaacata atacatgaac ggcttcagga gatatctgtg gagcttaaag agggtgaaat
     4021 atctgaaaag ctggcttctg cccatgcata tatgattgaa gcccaaaaaa atattaaaaa
     4081 agtcagggat gaaattaaaa gtatcggcgg tcatatacaa gagatctttt cctcaataga
     4141 cgatgctctt tcatacatgg agagcgcttt ggattatatg cttgacgcaa tgaatatcat
     4201 ggaaagggca caggatgata ttgatgatat ttttgacgga ataagagaac ttcttgaata
     4261 tctaaaagct caacccgagt ttgaattcca gactactgat gaattatatc aaaagacaaa
     4321 ggatgagctt tttacatcaa taggcagcat gtcagattcc ctttcctcat ttatggacga
     4381 tttgagcctg cagagcaata ctatgacgga tgatatgcaa tcagtaagcg accagctttt
     4441 taaggtgatg aatctggtga tcagcatttt tactgacttg agctttaatg aggaggacct
     4501 ggaagatata gttcaggatg tatccggtga ggatatagac aaaactacag aaggcaaggt
     4561 gtccggcagc agaaacctgg gaaatgttga aggagatata aatgtgggcg gaattgcagg
     4621 agccatgtct attgaatgga tgcttgaccc ggaggatgac ttggatttca aggaaaacct
     4681 gactgcaaac actgtatttc aaacccgcgt aattgttgac ggctgtacaa acgaaggcag
     4741 cattgtttcc aagaaaaaca atgcaggcgg aatagtcggc agcatggacc ttggatatat
     4801 caggaattgt cttggagccg ggtccattga aagcacagcg ggtaattatg tgggaggaat
     4861 agcaggaata tcaaaaggac cggttgtttc cagctatgca aagtgcagtc tccggggcgg
     4921 aaattatata ggaggcattt caggttttgg caaagagatt atcgacagct atacactggt
     4981 aaaggttaat gaatcaaggg catgtgtcgg tgccatagca ggagatatcg acaaaggcag
     5041 caacataaag ggaaattatt ttgtcagcga ttcaattcat ggcattgacg gcataagcta
     5101 tgctgaaaaa gctgaaccta ttgattatga tatactcata tcaaaagaaa atattccatc
     5161 ttcctttaaa gaattcaaac tgactttccg ggcagaagac aaactgataa aaaccataga
     5221 tttcaattat ggagaaagta ttaccattaa tgatttgccg gaggttccat taaaggaagg
     5281 ctattatgga aaatgggaag actttgacat gtcaaatctt acatttgatg atgagataca
     5341 ggctatgtac atacaccatg tgactgttct tgaaagcaat gagaagagag atgaaggttt
     5401 accggtaatt ctggttgagg gaaaattcac tgaagaagat tttattactc tcacaaagaa
     5461 tgaagtatca cccgacgacg aagggatact ggaacagtgg agaattgcaa tacctgatga
     5521 tggtgaaaat actcacatta taagatatta tccaatggaa gaaacaaata aaccgttagt
     5581 atatattttc agcagtggca gctggaatat ggcagatgcc cgacaggacg gaaaatatct
     5641 ggtatttgcc ggcgaaggaa acgatattac gttcagagta atcggaggga acttttacta
     5701 tgaatggatt tgggctggaa ttgctgctat ggtgcttatt gttacaacag cctgggtaaa
     5761 aagaagactt aagaaaaaag cctcagcgga aggctaagac ttttgtatat tttctgtctg
     5821 ggtaatgagc attccggcaa gcatgagggc agaacctaag tatcctctca taggcattgt
     5881 ttcgttcaaa atcagtattc cgcctatagc agcaaacact gcctccatgc ttaaggcaat
     5941 ggctgcgtgg gagggctttg catgtctttg tcccacagcc tgcagtgtgt atgcaactcc
     6001 tacagacatt atacctccgt acaatatagg aaccaaggca tcataaattc cgcttatact
     6061 gatatcttca aaaataatgg cagtaactat gcttaaaacc gagcaaacag caaattgaac
     6121 actggatagt tttatagcat tgacattctt gacaaattta tctattacct gaatgtgagc
     6181 cgcccagaaa aatgctccta ttaattgaag aaggtctcca aattgaattg tgaaatcttc
     6241 atttatactt atgaaatata gtcctacagc tgcagttact gcaccaatcc aggttgtagg
     6301 atatgttttt tgctttaaaa atattccaag tatgggtact aatactatgt ataaacttgt
     6361 gatgaatcct gcttttgctg cagtggtgta aataaggcca acctgctgga gggatgctgc
     6421 aataaacaat atgcatcctg caattgcccc tgattttatt gttgttttta agtctgcatc
     6481 aactttttca gcagaagaat tttctgcctt tgaacttttg tcaaaaaaat aaattacagg
     6541 caggagtgat acacttccca gggcaaatcg aatgccgtta aaagtaaaag cacctacata
     6601 ttccataccc atgcgctgtg ctacaaatgc cagaccccag atagctgctg ttaaaagcag
     6661 aatgaatact gattttattt cttttgtcat catgaaatcc ccttttatta ttaataacat
     6721 catcttaaca taaaaaaaag attatttaaa catcaatttt atcattttcc tcagtttttt
     6781 aatagcattg catttcatat ttattactga cctgtatttt aaatctaact cttctgaaat
     6841 gtcttggatt gaaaaccctt gaaaatagaa gaggtttatg attttcttct ctccaggggt
     6901 cagccccttt atgcacttgt gaagttcatt tttttcttct tctcttatta ttaaagctgc
     6961 cgggtctacc agggtggatt ttaacgtttt tcctgtttcg ttcaaatatt caaggctcaa
     7021 tgttctctgt tttatgtctt tcttgtagta gtttatatag taataataga gatttgtttt
     7081 aacatattgg agaaagtgaa catttctgtc aatgtcataa ttcttcaatg acttaagaat
     7141 cactatataa ccttcctgca gcaaatcctc aacaataggg tccgaaggtg cattatattt
     7201 ataaatgttt ttataaatga gcgggtttag tcttttgagc aatttttcaa tataaatctt
     7261 gtcccctttt aaagattcct gaataatata gttgatgtca gagtacattt ttatcacctt
     7321 gctttattat ataaatttat ataagtcagg taaaaatgtc agtaatcaaa taaagcaacc
     7381 tgaattgaac aaattcttaa attataaaat gttaaaaatt caataaattt attatctgaa
     7441 ggaaatgaag tatatgaaaa atgatataaa attcaacgaa attgaagcaa tgctgccggt
     7501 ctacgtgaaa gacaagggaa actgcacgga gataataaca aaagcagcat cttttataaa
     7561 aactgcaaca attgaaacat gcacaaagaa gatggcagac tattataatc ttagcatgta
     7621 tcataatcgg ataaactacg gacaagaact gggtattacc aataaagtgc ccctggttat
     7681 aaatgaaaac cttatttata tttacatcaa tgtaagagag cctatgtttg cccatgacgc
     7741 ggcttttgga ttatttgacg caaattcaat agacagtgtt tatgaagagg acggaaaggc
     7801 agtaattgaa ttgaaatccg gcaaaatgat caaaacaagg caaagtgtca aatccgtgaa
     7861 aaaaggcatg ctggatgcta gaattgcaaa agacatatac aaggaaaaac acaagatatg
     7921 agatgcaata agctctttag ctgaattatc ggggaaaaaa taataaaata cccgctcacg
     7981 gtttcggaca cgagtaattc taaagctcat gacc
//
LOCUS       DAOPUT010000068         7719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_9464_length_7719_cov_19.898747, whole genome shotgun sequence.
ACCESSION   DAOPUT010000068 DAOPUT010000000
VERSION     DAOPUT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9464_length_7719_cov_19.898747"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKK75_06345"
     CDS             complement(<1..254)
                     /locus_tag="PKK75_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNZ83254.1"
                     /translation="MKSLEELRKLRDEAVKNVEMRNTGKDVRVVVGMATCGISAGARP
                     VLSTFVEEVAKRNLNNVSVVQTGCIGMCTYEPIVEVYTPD"
     gene            complement(265..750)
                     /gene="nuoE"
                     /locus_tag="PKK75_06350"
     CDS             complement(265..750)
                     /gene="nuoE"
                     /locus_tag="PKK75_06350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNZ83255.1"
                     /translation="MTKALDVKANEKNFIELKEYIETVKGANGNLMPVLHEAQEIFGY
                     LPIEVQKFISSELDVPLAEVYGVATFYTRFAIEPKGKHKIGVCLGTACYVKGSQLIMD
                     KLSKELNIKVGQTTPDNLFTLEATRCLGCCGLAPVMMIDQDVYGRLEPKKIPEILGKY
                     K"
     gene            complement(1046..1888)
                     /locus_tag="PKK75_06355"
     CDS             complement(1046..1888)
                     /locus_tag="PKK75_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNZ83256.1"
                     /translation="MAAKRKALGKGLSALIPEDLEKEDYERIHEIDIDLISPNPNQPR
                     RVFEQGKLDELTESIKKYGVIQPIILKKEGDLYTLIAGERRWRACKNANIKTIPSIVR
                     DIENRNASEIALIENIQREDLNPIDEANAYEFVMDRYGITQEQVSNIVGKSRVYVTNI
                     MRLNNLDEYVKDKIVKNLITAGHGRAILSFDKEKQKSITDIIIRDNLSVRDVEKLVRT
                     SKKPKRKKAEKDKYIVYLEEMLTEKFSTRVNIKNKKNKGKIEIEYTGNDDLNRILDLL
                     KIED"
     gene            complement(1891..2664)
                     /locus_tag="PKK75_06360"
     CDS             complement(1891..2664)
                     /locus_tag="PKK75_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ83257.1"
                     /translation="MAKVISIFNQKGGVGKTTTNVNLCAALALKGKKILSIDIDPQGN
                     TTSGFGFSKNQLEYTIYNVLIEDYDINKVIFKTEIENLDLIPANIQLAGAEIELASAR
                     FREKTLKEKMNLIENKYDFIILDCPPSLGLLSLNALTASNTVLIPIQCEYYSLEGVGQ
                     LIDTVKLVKKNLNPRLEIEGVILNMFDGRTNLSIQVVEEVKKYFKDKVYRTVIPRSIR
                     LAEAPSFGQPIMLYDDKSKGSIAYTKLSEELIKNNRRTV"
     gene            complement(2676..3464)
                     /gene="noc"
                     /locus_tag="PKK75_06365"
     CDS             complement(2676..3464)
                     /gene="noc"
                     /locus_tag="PKK75_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HNZ83258.1"
                     /translation="MLNNIVHINVNKIIPNKNQPRKNFDEKALEELSQSIKSYGIIQP
                     ITVRKIYDDIYEIVAGERRYKAVKMLNLDTIPAVVIEVKEEESAAMSLIENLQREDLH
                     FIEEAAAYERLTREFGLSQSQLAEKLGKSQSAIANKVRILKHPEQVKEKLREGNLTER
                     HARAMLKITDEEILLNLIEKVVSKDLNVSETEKLVDSIANELNDRKKKDKRYVRNFIN
                     YKVYINTIKNAYNEIVKTGIDAKFEQNESEDYIEIKVKIPKKSV"
     gene            complement(3475..4188)
                     /gene="rsmG"
                     /locus_tag="PKK75_06370"
     CDS             complement(3475..4188)
                     /gene="rsmG"
                     /locus_tag="PKK75_06370"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNZ83259.1"
                     /translation="MIKTLEEGFSLLKLPYNKDIDSKFALYRDLLKEWNKKINITSIE
                     DDEEIYIKHFIDSVLLLNENNINEKKTVIDVGTGGGFPGLPLKIVNDNYKITLLDSLK
                     KRIDFLSQVTKALDLSDVNLIHERAEDYGQNKEIRECYDICVSRAVAPLNVLSEYCIP
                     FVKVGGYFAAYKSDNISQEISNSDNAIKKLGGKIKEIKEISIPATDIIRKIVIIEKIE
                     PTNTRYPRKAGKPNKDPLK"
     gene            complement(4200..6089)
                     /gene="mnmG"
                     /locus_tag="PKK75_06375"
     CDS             complement(4200..6089)
                     /gene="mnmG"
                     /locus_tag="PKK75_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNZ83260.1"
                     /translation="MEEKVREFLEESIDVVVIGAGHAGCEASLACARLGLKTIMLTLS
                     LDAIAMMPCNPNIGGTAKGHLVREIDALGGEMGLNIDKTMIQCKMLNRSKGPAVHSLR
                     AQADKKKYHTEMKKTIENQKNLRVIQTEATKISLNEDKSFNVYTKLGAYYRAGAVIVT
                     TGTYLKGRIYMGELNYSSGPDGLAPANELSGSLMDLGIELVKFKTGTPARIHKDSIDY
                     RKMALQTGDDEIIPFSFMNDEINIDQVPCYVTYTTKETHELIRRNLDRSPLYGGVIKS
                     VGPRYCPSLEDKVVKFSDKEKHQLFIEPEGLDTKEMYIQGMSTSLPYEIQIQMYRSMI
                     GLENAEIMRPAYAIEYDAIDPTQLKLNLELINIKNLFFAGQVNGSSGYEEAAAQGLMA
                     GINAYLSLKGRDPLILDRSEAYIGVLIDDLVTKGTNEPYRMMTSRAEYRLILRQDNAD
                     ERLTQKGYDVGLVTEERYKRYKDKKQRIENEIERLKNIKITPKAEINEALENMGSSAL
                     KFPTDLFDLLRRPELDYDNTLFLDRERPVLRKETKDYVETVIKYEGYIQKQVAQVDQF
                     KKLENKKIPNDIDYDNVGSLRIEARQKLNKIKPDSIGQASRISGVSPADINVLLIYLM
                     THNQK"
     gene            complement(6167..7540)
                     /gene="mnmE"
                     /locus_tag="PKK75_06380"
     CDS             complement(6167..7540)
                     /gene="mnmE"
                     /locus_tag="PKK75_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNZ83261.1"
                     /translation="MFSDTIAAIATFPGNAGINIIRLSGTDALNIAYKIFVDKNRQKP
                     ENLKARYLHYGYIEDEKGKLIDEVLISYMKAPNTYTREDIVEINCHGGIISAKKILDT
                     VLHYGCRPAERGEFTKRAFLNGRIDLTQAEAVIDIINSKTDSSHEISVKHLEGRLSKQ
                     INAVIDEIMDLLANIEVNIDFPEYDEDEITIERVKALCERLGPELDKLIKTADTGKIF
                     KEGIKTVILGKPNVGKSSLMNFLLNENRAIVTEVPGTTRDTIEEYVNIKGVPLRIIDT
                     AGIRETEDRVERIGVEKALEKVSEADLVIMIFDSSKELEKEDESILDYIKDKKVIYIK
                     NKTDLENKLDLSSYEKIEDEIISISVLQNQGLDDIIDKINSLFFEGHLNVTDELIINN
                     VRHKNLLIKAKKSIYEVLISIENSMPIDFIEIDLKQAMESLGFIVGKSVSDDLVDKIF
                     SEFCIGK"
BASE COUNT         2033 a         1609 c         1136 g         2941 t
ORIGIN      
        1 ttgtcgggcg tataaacttc tactattggc tcataggtgc acattcctat gcagcctgtc
       61 tgtacaaccg aaacattgtt tagatttctt tttgcaactt cttcaacaaa tgttgaaaga
      121 acaggtcttg ctcctgcaga tatcccgcag gttgccatgc ccaccacaac acgaacgtct
      181 ttacctgtat ttctcatttc aacattctta actgcttcat ctcttaactt tcttaattct
      241 tccaatgact tcatgatatc cctcctactt atattttccc agtatttcag gtattttttt
      301 aggctctaat cttccgtata catcctggtc tatcatcata acaggagcaa gaccgcagca
      361 gcctaagcat cttgtagctt cgagagtaaa taaattatcc ggcgtagttt gtcctacttt
      421 gatattcagc tcttttgaca gcttatccat tatcagttga gagcctttta cataacatgc
      481 tgtgccgaga catactccga ttttgtgctt ccccttaggt tcaatggcaa atcgtgtata
      541 aaatgtagcc acaccataca cttctgctaa cggaacatct aattcggaag aaataaactt
      601 ttgaacttca ataggcaagt atccgaatat ttcctgagcc tcatgcaaaa ccggcattaa
      661 gttgccgttt gcacccttaa ctgtttcaat gtactcctta agctcaataa agttcttttc
      721 atttgccttt acatcaagtg cttttgtcaa aataaactac ctcctcatgg cgttttctta
      781 tatgagaata ttatatattt tcgttaataa aatgtcaatt catttaggaa acattttttt
      841 aaagtatgca aatttgataa tatagttact tttgttaata caatatagtt ttacttatgc
      901 ctttattatt atgtttaatt aatatgtttg atgaagtaat aaaatatata ttcggcaaaa
      961 aataaaaaag aatcattagt taaataaaaa acggaaaaaa gactctgaat ttaggagcag
     1021 attataagta ttataagggc tgcacctagt cttcaatttt taataggtcg agtattctgt
     1081 taagatcgtc gtttccagtg tattcaattt ctattttccc tttgtttttt ttgtttttaa
     1141 tattaaccct tgtggaaaac ttttcagtca gcatttcctc caaatatact atgtatttat
     1201 ctttttcagc ctttttgcgt tttggctttt tagaagtacg caccagtttt tctacgtctc
     1261 tcacgcttaa attatctctt attattatat cggttattga cttttgcttt tccttgtcaa
     1321 agcttaaaat agcccgtccg tgaccggcag ttattaaatt ttttacaatt ttatccttaa
     1381 catactcgtc taaattatta agcctcatta tgtttgtaac gtatactctt gattttccga
     1441 cgatgtttga tacctgttcc tgggtgattc cgtatctatc catgacaaat tcataagcat
     1501 ttgcttcatc tatgggattt aaatcttctc tttgaatatt ttcaatcaat gctatttcag
     1561 aggcatttct gttttctata tctcttacta tggaaggaat tgtttttata ttagcatttt
     1621 tgcaggctct ccatcttctt tcccctgcaa ttaatgtgta gaggtctcct tcctttttca
     1681 aaattatagg ctgtatcaca ccgtatttct taatggattc agtaagctca tcaagcttgc
     1741 cctgctcaaa aaccctgcgg ggctgatttg gattagggga aatcaaatca atatctattt
     1801 catggattct ttcataatct tccttttcca aatcttcggg tattaatgcc gacaagccct
     1861 ttcccaatgc ttttctctta gcagccatat ttacacagtc ctcctattat ttttaatcaa
     1921 ttcttcagac aattttgtat atgcaatgct tccttttgat ttatcatcat agagcattat
     1981 aggctgacca aagcttggcg cctctgcaag ccttatgctt ctgggtataa ctgtcctgta
     2041 taccttatcc ttaaaatatt ttttgacttc ttcaacaact tgtattgaaa gattggttct
     2101 gccgtcaaac atatttaaaa taactccttc aatttcaagc ctggggttta aatttttctt
     2161 cacaagctta actgtatcaa taagctgtcc tactccttcc aatgaataat actcgcattg
     2221 tatgggtata agtaccgtat tggatgcagt cagggcattt aatgacaaga gccccaggga
     2281 cggggggcag tccagaatta taaaatcata tttattctca attaaattca ttttttcctt
     2341 cagtgttttt tccctgaacc tggcactggc cagctctatt tccgcccctg ccagctgtat
     2401 atttgccgga ataaggtcca ggttttctat ttctgtcttg aaaatgacct tgttgatgtc
     2461 gtaatcttca atcaatacat tgtaaattgt atactccagc tgattcttgc tgaatccgaa
     2521 tccgcttgtt gtattgcctt ggggatcaat atcaatgctg agtatcttct ttccttttaa
     2581 agccaatgcc gcacaaagat taacattggt tgttgtcttc cccacaccgc ctttttgatt
     2641 gaaaatactt attaccttgg ccatattatt atcctctaca cactcttttt aggaattttc
     2701 acctttattt caatgtaatc ctctgattcg ttctgttcaa acttagcgtc aataccggtt
     2761 ttaactattt cattgtaagc attttttata gtatttatat ataccttata attaataaag
     2821 ttcctgacat aacgcttgtc cttttttttc ctgtcattca actcattggc aattgaatcc
     2881 acaagcttct cggtttcact tacatttaaa tcctttgaaa ctactttttc gataaggttt
     2941 agaagaattt cctcatcggt tattttcaac atggctcttg catgcctctc ggttaaattt
     3001 ccttccctta atttttcttt aacctgctca gggtgtttaa gaattcttac tttattagca
     3061 attgctgatt gtgacttgcc gagtttttca gccagctggg actggcttag tccgaattcc
     3121 ctggttagtc tttcgtaggc tgctgcttcc tctataaaat gcaggtcctc tcgctgcaaa
     3181 ttttcaatca gggacatagc tgcggattct tcttccttaa cttcgataac cactgcggga
     3241 attgtatcca gattaagcat ttttacagcc ttataccgcc tttctcccgc tacgatttca
     3301 taaatatcat catagatttt tcttaccgta ataggctgta taattccata actctttata
     3361 gactgactta attcctccaa tgccttttca tcaaaatttt ttctcggctg attcttgtta
     3421 ggtattattt tgttaacatt aatatggaca atattattga gcattttctc ccccttattt
     3481 taacggatcc ttgtttggtt tcccggcttt tctcggatac cttgtatttg ttggttctat
     3541 cttctctatt ataacaattt ttctgataat atcagtagcc ggaatagaaa tttctttaat
     3601 ttctttaatt tttccgccaa gtttcttaat tgcgttgtcg gaatttgaaa tttcctggga
     3661 aatattgtcg cttttatagg ctgcaaaata tcctccaacc tttacaaatg gaatgcaata
     3721 ttcgcttaaa acattaaggg gggccaccgc tcttgaaaca catatatcgt agcattccct
     3781 gatttcttta ttttgaccgt aatcttcggc tctttcgtga ataaggttta catcgcttaa
     3841 atccaaagct tttgtcactt gggacaaaaa gtctattctt ttttttaaac tgtccagcag
     3901 cgtaatttta taattgtcat tgacaatttt aaggggcagt cccggaaaac ctcctccggt
     3961 gcctacatca atcacggttt tcttttcgtt aatattattt tcattcaata gaagaacgct
     4021 gtcgataaaa tgctttatat atatttcctc atcatcctct atggatgtaa tatttatttt
     4081 tttgttccat tcctttaaaa ggtccctgta taaagcaaat ttactgtcaa tatctttatt
     4141 atagggtaat ttaagcaggc tgaaaccctc ttccaatgtt tttatcaaag tgaactcctt
     4201 tatttttgat tatgggtcat cagataaatt aaaagaacat ttatatctgc aggtgaaact
     4261 cctgaaattc tggaagcctg gcctatggag tccggcttta ttttgttaag tttctgcctt
     4321 gcttcaattc ttagacttcc tacattatcg tagtctatgt cattgggaat ttttttgttc
     4381 tcaagctttt taaactggtc tacctgggct acttgttttt gtatatagcc ttcatatttt
     4441 attactgttt ccacataatc ctttgtttct ttcctgagta caggtctttc cctatccaaa
     4501 aataatgtgt tgtcataatc cagttccggc cttctcaata aatcaaaaag gtctgtagga
     4561 aattttaaag cagaacttcc catattttcc aaagcttcgt ttatttcagc cttgggtgtt
     4621 attttaatat tttttaatct ttctatctca ttttcaattc tttgtttttt atccttgtat
     4681 cttttatatc tttcttctgt gaccaatcct acatcatagc ctttttgcgt aagtctttca
     4741 tcagcattgt cctgtcttaa aataagcctg tactctgccc ttgatgtcat cattctgtag
     4801 ggctcatttg ttcccttggt aaccaggtca tcgatcagca ctcctatata ggcttctgaa
     4861 cggtctaaaa tcaaaggatc tcttcccttt aggcttaaat atgcatttat tccagccatc
     4921 aagccttgtg cagcggcttc ttcatagcca gagcttccat ttacctgtcc tgcaaaaaat
     4981 aaatttttaa tgttaattaa ttcaaggttc agcttaagct gggtcgggtc gatggcatca
     5041 tattcaatag cataggcagg cctcataatt tctgcatttt ccaggccaat catggacctg
     5101 tacatttgaa tctgtatttc gtaaggaaga gaggtagaca taccctgtat atacatttcc
     5161 tttgtatcga gcccttcagg ctcaataaaa agttgatgtt tttccttgtc tgaaaatttt
     5221 accaccttat cttccaatga ggggcagtac cttggtccaa cgctttttat aacacccccg
     5281 taaagaggag atctatctaa attcctcctg attaattcat gagtttcttt tgtagtgtag
     5341 gtaacatagc agggcacctg gtcaatattt atttcatcat tcatgaatga aaaaggtatt
     5401 atttcatcat cgcctgtttg caatgccatc ttccggtaat caatcgaatc cttgtgtatt
     5461 cttgcaggtg ttcccgtctt gaattttaca agctcaatcc ccagatccat taatgaaccg
     5521 gataattcat ttgcaggagc cagaccgtca ggtcccgatg aatagttcag ctcacccata
     5581 taaattcttc cctttaaata tgtgcctgta gtaactatga cagctccagc cctgtagtat
     5641 gctccaagtt tggtatatac attgaagctt ttatcctcgt ttaaacttat ttttgttgct
     5701 tcggtctgaa taactcttaa attcttctgg ttctcaatcg tttttttcat ttcagtatga
     5761 tacttttttt tgtctgcctg tgctcttaag gaatgtaccg caggtccttt tgaccggttc
     5821 agcatcttac attgtatcat agtcttgtct atatttaaac ccatttcccc gccgagagca
     5881 tcaatttccc tcaccaggtg gccctttgcc gttcctccta tattggggtt gcagggcatc
     5941 atggctattg cgtcaaggct taaagtaagc attattgttt ttaaaccaag ccttgcacag
     6001 gccagggatg cttcacatcc tgcatgacct gcgccaatta ccaccacatc tatgctctct
     6061 tcaagaaatt cccttacttt ttcttccata cttgttcctc tcttaattga ggacattcct
     6121 tttagtattt ctaagtagat tttcttctaa aaggtgtatc cctttcctat ttacctatgc
     6181 aaaactcact gaagattttg tcaaccaaat catcgctgac tgattttccc acaataaatc
     6241 ccaagctttc cattgcctgt ttcaaatcaa tctcaataaa atcaattggc atactattct
     6301 ctattgaaat taaaacttca taaatagatt tttttgcttt tatcaataag ttcttatgtc
     6361 ttacattgtt aataataagc tcatctgtta catttaaatg tccttcgaaa aacaaagagt
     6421 ttattttatc tataatatca tccaagccct gattttgtaa aacggatatg ctgataatct
     6481 catcttctat tttttcgtaa gatgacagat caagcttatt ttccaaatca gttttatttt
     6541 ttatataaat taccttttta tctttaatat agtctaatat gctttcatcc tctttttcaa
     6601 gctcctttga tgaatcaaaa atcattatta caaggtcagc ttcacttacc ttttccaaag
     6661 ccttttcaac acctattctt tctaccctgt cctcggtttc ccttattccc gcagtatcta
     6721 ttattcttaa gggaactccc ttgatattta cgtattcttc tatggtatcc ctggttgttc
     6781 cgggaacctc cgttacaatt gcacgatttt cattcaaaag aaaattcatg agagatgatt
     6841 ttcccacatt gggcttcccg agtattactg tttttatacc ttccttgaat attttcccgg
     6901 tgtctgctgt ttttatgagt ttatcaagtt caggccccag tctctcgcat aaagccttaa
     6961 ctctttcaat agtaatttca tcctcgtcgt actcgggaaa atcaatatta acttcaatat
     7021 ttgccaataa gtccattatt tcatcaataa ctgcgtttat ttgttttgac agcctgccct
     7081 ccaaatgttt tacggaaatc tcatgactgg agtcggtctt tgaatttatt atatctatta
     7141 ctgcctctgc ctgagttaag tcaattcttc cgtttaaaaa ggccctcttt gtaaattctc
     7201 ctctttcagc cggcctgcag ccgtaatgaa gtactgtatc cagtatcttt ttagcggaaa
     7261 taattcctcc gtggcagtta atttcaacaa tatcttccct tgtatatgtg tttggagcct
     7321 tcatgtaaga tatgagaact tcatctatta actttccttt ttcatcctca atatatccgt
     7381 aatgaagata tcttgctttt aagttctcag gtttctgtct gtttttgtca acaaaaattt
     7441 tatatgcaat atttaatgca tccgttccgc ttaatcttat tatattaatg cctgcattgc
     7501 caggaaatgt tgcaattgca gcaattgtat cggaaaacat atatccacag cttctttcta
     7561 tcaatataga tacctgtatt ttatcttaat tatatataaa agtacatata tttaaattat
     7621 ttatataaaa aatgttgcta ttcatgatta ttaagaaaac atctataaat attaagcgaa
     7681 catttgcaga acacgattat aattcttagc gaatgcaag
//
LOCUS       DAOPUT010000069         7239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_10289_length_7239_cov_18.051782, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000069 DAOPUT010000000
VERSION     DAOPUT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10289_length_7239_cov_18.051782"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKK75_06385"
     CDS             complement(<1..429)
                     /locus_tag="PKK75_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83262.1"
                     /translation="MYRLFPKIDYLNIEEVKLLNTYRYLSWILTSIVYYMNYPSDKLI
                     KLGIIAVLFVTSRIVVNVYKRYDINSKVDSAFVFIETIGITLILLPTGGLDSPFIWYA
                     LNPVLISAYFFTNLFCWFNLFCYLSCSTAVSYLFFNTSSLS"
     gene            760..1035
                     /locus_tag="PKK75_06390"
     CDS             760..1035
                     /locus_tag="PKK75_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HNZ83263.1"
                     /translation="MGFAETFKALSDPVRREILLMLREGKMSAGEIAEKFDMSPATIS
                     YHLSQLKKAGLLFETKYKNYIYYEINVTVFEELMLWFTQFGGIENEN"
     gene            1025..1663
                     /locus_tag="PKK75_06395"
     CDS             1025..1663
                     /locus_tag="PKK75_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007127514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HNZ83264.1"
                     /translation="MKIKVDKLLIISTLICLLPIILSLIVYDKLPDTMPIHWDIEGNP
                     DGYGSKAFAAIWLPLMMAGLNLITHFGLNTDPKKANSSKILKLIGKLTIPFLSLTLLP
                     ITIFAGLGYEIAIERIVPAFVGLLFIIVGNYLPKSKQNYTVGIKLPWTLDNETNWNKT
                     HRLAGYLWIIGGVVMFVNSFLQIYWTPVFFIIIGSMVFIPALYSYILYRNGN"
     gene            complement(1954..2556)
                     /locus_tag="PKK75_06400"
     CDS             complement(1954..2556)
                     /locus_tag="PKK75_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HNZ83265.1"
                     /translation="MNFTTKERFFLEDQKSQEEICVQKYTNYAGMASCQELKSIFQQN
                     GQKEQEHYNTIDQFLKGQMPSVSQSGSKQSGSQQSGGQQSWQMKSGGQSSSQPGGQSS
                     SKQSVSQQSGGQQSWQMKSGGQSFQPQQGSSGYNNSDKDLCTDMLMTEKYVSGAYDTA
                     IFEFRDPQVRSILNHIQKEEQQHGEAIFKYMESKGMYQAK"
     gene            complement(2725..4086)
                     /locus_tag="PKK75_06405"
     CDS             complement(2725..4086)
                     /locus_tag="PKK75_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNZ83266.1"
                     /translation="METLMKINSTVNGIVWGPLIIVLIIGTGTYLSAMTGFFSITKLG
                     YVLKNTLFKMFTKEHKGEGEVTAFQAVATALAATVGTGNIAGVATAIALGGPGAVFWM
                     WLAAVMGMTTKFAEVVLAVNYREKTPDGRFVGGPMYSITNGLGKNWKWLGVVFAIFGA
                     LASFGIGCMVQSNSVAFAMEGTFGVSPIITGIILAVITAVVIIGGIKRIGAFTEKLVP
                     FMAAVYIVGGLWVIIANAGKLPNAFSMIFGQAFTGTAAVGGFAGSTMMQAVRYGVARG
                     IFSNEAGLGSAPIAHAAATTDHPVRQGLWGVFEVFMDTIVICSITALVILVSGVWDNG
                     QSGAVLSATAFDVGLPGIGKYIVSVGLILFAYSTIIGWEYYGERCVEYLLGTKPIMAY
                     RILWVVAVVVGSIGGLTFVWDLADTLNALMAFPNLIGVLMLSPVVFRLTKEFWAMEKD
                     KAK"
     gene            4398..4982
                     /locus_tag="PKK75_06410"
     CDS             4398..4982
                     /locus_tag="PKK75_06410"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNZ83267.1"
                     /translation="MKIYDFTVKDMDNKDVSLSNYMGKVLLVINGATGCGFTPQYDGL
                     QKLYDKYAPEGFEILDFPSNQFLEQAPGTNDEIANFCRLNFGTEFKQFSKIEVNGPNE
                     DPLYKYLKTTAEEFRNQGSVDFYSRVKKYTPDIKENDIRWNFTKFLIDREGNVIARFA
                     PNITAEEIDGHIASLLRNEEIEIVCHPDFSQGCI"
     gene            complement(5063..5752)
                     /locus_tag="PKK75_06415"
     CDS             complement(5063..5752)
                     /locus_tag="PKK75_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ83268.1"
                     /translation="MVLTVENLSKSFGKSMVFHDISFSVNKGEIVCIRGKSGEGKTTL
                     LRCLTSLETPDKGSIKINNMYLCREENSQIKYAQKGELQAIRQEIGLVFQDFNLFPHM
                     SVMENLMEAPVFLKNDSPDNIRKRALELLKSLELEGKEDSYPFQLSGGQQQRVAIARA
                     CMLNPSVLCFDEPTSALDEQTRGQISKIIRYLSSEGISIIIVTHDNAFADEIAQRIIT
                     MKDGKITIQEQ"
     gene            complement(5763..6419)
                     /locus_tag="PKK75_06420"
     CDS             complement(5763..6419)
                     /locus_tag="PKK75_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNZ83269.1"
                     /translation="MESKVLTMLVPMATEGLKVTLGVFALTLIMSLPLAIVVAKLRMS
                     KNKIISKITGIYIYIMRGTPLLLQLMFIFFGYYRVPLIGYPFSRGQAIVIAFVLNYTA
                     YFAEIFRGGINSIDIGQHEASLVLGLSKTHTFMKIILPQVIKNVMPSIGNEVITLVKD
                     TSLVYILGSSDILKAARSVSNVYAAFSPYIYIGLVYLVIIAILTKVLDKIENRYDYYR
                     "
     gene            complement(6429..7208)
                     /locus_tag="PKK75_06425"
     CDS             complement(6429..7208)
                     /locus_tag="PKK75_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005216079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ83270.1"
                     /translation="MKKSVLKSILAAVLLVLIAVMAAGCAQEAQKETEELVMGLDDTF
                     APMGFRDEKGNLVGFDVDLANEVAERIGVTMKFQPIDWSMKETELNGGNIDLIWNGYT
                     ITAERQEKVAFTKPYLENSQIIVTMADSDIVTKADLAGKNVTVQAESSALDAINAEPD
                     VAASFGELVEFATNNEAFSDLESGRSDALVVDEVNARYYMKQAGEEKYKVLEEDFGDE
                     EYGIGLRKDDTELLKKINDAMDEMKSDGTYDEIYEKWFAEN"
BASE COUNT         2227 a         1458 c         1270 g         2284 t
ORIGIN      
        1 gctaaggctt gaagtattga aaaacaaata agatactgcc gtagagcagg aaagataaca
       61 aaataaattg aaccagcaaa ataagttagt aaaaaagtag gctgaaataa gcacaggatt
      121 taaagcgtac catataaaag ggctgtccaa accccctgta ggtagaagaa ttaaagtaat
      181 tcctatggtt tcgataaaaa caaaagctga gtccaccttt gaattaatgt catatctttt
      241 gtatacattc acaacaattc ttgaagttac aaataagaca gctattatcc caagctttat
      301 cagcttgtcc gaaggataat tcatatagta tacgattgaa gtaagaatcc atgataaata
      361 cctgtaagta ttcaataatt taacttcttc aatattcaaa tagtctattt tgggaaacaa
      421 tctatacatg ataacctgcc tgatattgtt tgattttatt gtaagacatt agctaagaat
      481 aatcaattaa aaattgttgt atgatatcat attatgtcga aaccgcatat tctcttcatc
      541 taaaggtaag ggaacacctc ttgtaggacg gggcatacct tcctggcaag taatcaataa
      601 gaccggtcat gcaggaatgt tcctgctggg atagtcttcc cgcatgggtg catgttacaa
      661 aaatattatt tcgaaaattt tcgaaatagg tattgacagt tattttctta tttagtatat
      721 tctatttaga aataattcga aatagaaaga ggtgatactg tgggatttgc agaaacattt
      781 aaagcgcttt ctgacccggt aagaagagaa atattattaa tgctgagaga agggaaaatg
      841 tccgcaggag aaatagcaga aaagtttgat atgtcccctg ctacaatttc atatcacttg
      901 tcgcagctta aaaaagcagg actgttgttt gaaactaagt ataaaaatta catttattac
      961 gaaatcaatg ttacagtatt tgaagaatta atgttgtggt tcacacagtt tggaggtatt
     1021 gaaaatgaaa attaaggtag acaaattatt gataataagt actcttattt gtttgcttcc
     1081 tataatattg tcattgattg tgtatgataa actgcctgac acaatgccca tccactggga
     1141 catagaaggc aaccctgacg gctacggttc taaagccttt gctgccatat ggcttccctt
     1201 aatgatggca ggcctgaatt taattaccca ttttggattg aatactgatc ccaaaaaagc
     1261 aaattcctca aaaattttaa agcttatcgg aaaactgacc atacctttcc tttctcttac
     1321 tttgctgcca ataaccatat ttgccggttt gggatacgaa atagccattg aaagaattgt
     1381 accggctttt gtcgggttgt tatttataat agttgggaat tacttgccta agagcaaaca
     1441 aaactatact gttgggataa aactgccatg gactttggat aatgaaacaa attggaacaa
     1501 aacacaccgt ctggcaggat atttgtggat tatagggggt gtagtgatgt ttgttaacag
     1561 ttttctgcag atatactgga cacctgtatt ctttataata atagggagca tggtttttat
     1621 accggctctt tactcctata ttctgtacag aaatggaaat taaaggtgtg gggacacacc
     1681 ttaatggcgg atagtctatg aggtatgcat tgaggaatgt ccccaatagg agaatacagc
     1741 ttaaatacta ctaaagtagt ataaaaaaat atcattttga gtgatgttaa gttttaaaaa
     1801 ttaaactata attaaattat cattagataa ctctaagggg gagttgttta aaaataatta
     1861 tttgacaatc ttatcattca acagagctgc aaatgtctat ctcttctaag cagctctgtt
     1921 ttattggtta aaaaaagtgg ctgagccact tttttatttc gcttgataca ttcccttgct
     1981 ttccatatac ttgaatatag cttcaccatg ctgctgttct tccttttgaa tatggtttaa
     2041 gatacttctt acctggggat ctctaaattc aaatattgcc gtatcatatg cacctgatac
     2101 atatttttca gtcatgagca tatctgtgca aaggtccttg tcggaattat tgtagcctga
     2161 acttccctgc tggggctgga atgactgccc gcccgacttc atctgccagc tctgctgacc
     2221 acctgactgc tggctgaccg actgcttgct gcttgattga ccaccgggct ggctgcttga
     2281 ctggccgccg gacttcatct gccaactctg ctgaccccct gactgctggc tcccggactg
     2341 cttgctgccg gattgactca ctgacggcat ctgacccttt aaaaactggt caattgtgtt
     2401 gtaatgctcc tgctccttct gcccgttttg ctggaaaatg cttttcagtt cctgacatga
     2461 tgccattccg gcatagtttg tgtacttttg aacacaaatt tcttcctggg atttttgatc
     2521 ttctaaaaaa aatcgttctt tagttgtaaa attcattcga acacctccga ttatattatt
     2581 ttcaaaatac caaaatatat ccctaaataa ttaagttaat tcagttatat aattaaaaaa
     2641 attgcagaat aaactgcaac ttaccgcaaa taaaaaaagc gctgattttt attttaatca
     2701 gcgccttctg gattataatc cttattattt tgctttgtct ttttccattg cccagaattc
     2761 ttttgtcagc ctgaatacta ccgggctcag catcaggaca cctatcaagt tcgggaatgc
     2821 catcagagca tttaatgtgt ctgccaaatc ccatacaaat gtaagtccgc ctatagatcc
     2881 tactacaact gctacaaccc ataatattct gtatgccatt atcggttttg ttccaagcaa
     2941 gtattcaaca catctttcac cgtagtattc ccagcctata attgttgaat atgcaaacaa
     3001 tataagaccg acacttacta tgtacttgcc tattcccgga agacctacat caaatgcagt
     3061 tgcagaaaga accgctccgc tttgtccatt atcccaaaca ccggaaacca aaataacaag
     3121 agcagtaata gagcagatta caattgtatc cataaatact tcgaatactc cccacaatcc
     3181 ctgacgaacc gggtggtctg tagttgcagc ggcgtgtgct ataggagcgc ttcctaagcc
     3241 tgcttcgttt gagaaaatac cacgtgcaac accgtaacgg actgcctgca tcatagttga
     3301 gcctgcaaaa cctcctactg cagctgttcc tgtgaatgcc tgaccgaaaa tcattgaaaa
     3361 agcattaggc agtttgccgg cattagctat aatcacccaa agaccgccga ctatataaac
     3421 tgctgccata aagggaacca atttttcggt gaaggcaccg attctcttaa taccgcctat
     3481 tataacaact gctgttatta cggctaatat tatacctgta atgataggac ttacaccaaa
     3541 tgtaccttcc attgcaaagg caactgagtt tgattgaacc atacagccta taccaaagga
     3601 tgctaaggca ccgaatattg caaacacaac gcctaaccat ttccagttct tacccagacc
     3661 gtttgtaatg gaatacatgg gaccgcctac aaaacggccg tctggagttt tttccctgta
     3721 gtttacagcc agaacaactt ctgcaaattt tgtagtcata cccataactg cagcaagcca
     3781 catccaaaat accgctccag ggcctccaag agctattgct gtagcaactc cggcgatgtt
     3841 acctgttccg actgttgctg caagagctgt ggcaactgcc tggaatgccg taacttcacc
     3901 ctctccttta tgttcttttg tgaacatttt aaataaggtg ttttttagaa cgtagcctaa
     3961 tttagtaatt gagaaaaatc cagtcatagc tgataaataa gttccggttc caataattaa
     4021 aactataatt agtggccccc atacaatacc gttaaccgtg ctgttgattt tcatcagtgt
     4081 ttccaaacaa atccctccat ttcggataat atgataaacg tttacaactt ttagcatttg
     4141 agtatataaa agatttctga atagaagtta aatataaaaa gttgaaacgt tttcaataaa
     4201 tattatatag gatttaacaa aataattcaa caaaaattat tacatttctt tgttatattt
     4261 ttttcataat tgcttaacta ttttgagata gactatatct gaaacttata agcaggtcta
     4321 taatcaaaaa ttctgcttaa agacaataaa agggggtatt aatatattat agatcaaaca
     4381 ggaggaaaaa ggataatatg aaaatatatg attttacagt taaagatatg gacaataagg
     4441 atgtgtccct gtcaaattac atgggaaagg tattgcttgt aataaacggt gcaacaggct
     4501 gtggctttac acctcaatat gacggacttc aaaagctata tgacaagtat gcccccgaag
     4561 ggtttgaaat tttggacttc ccaagcaacc agttccttga gcaggctccc ggtacaaatg
     4621 atgaaatagc aaatttttgc agattaaatt tcggaacgga atttaaacaa ttttcaaaaa
     4681 tagaagttaa cggtcccaat gaagacccct tgtacaaata tttaaaaaca actgctgaag
     4741 aattcagaaa ccagggaagt gttgattttt acagcagggt taaaaaatac actcccgata
     4801 taaaagaaaa tgatatcaga tggaatttta ctaaattcct catagacaga gaaggaaatg
     4861 tcattgcaag atttgcaccg aacatcactg ctgaagaaat agacgggcat atagcaagct
     4921 tgcttcgaaa tgaagaaatt gaaattgtat gccacccgga cttttcacag ggctgcatat
     4981 agagactaca gcattgacta aaaaagcgct gagctaatca gcgctttttt gaaagttcgg
     5041 gaagaccatc aaaaaaaaag agctattgct cttgtattgt aatttttcca tccttcattg
     5101 taattattct ttgtgctatt tcatctgcaa atgcattgtc atgggtgaca atgattattg
     5161 agataccttc cgaagacaaa tacctgatta tttttgatat ctgtcctctt gtttgttcat
     5221 ctaaggcaga tgtgggctca tcaaagcaca atactgaagg attaagcatg catgccctgg
     5281 ctattgccac acgctgctgc tggcctcccg atagctgaaa gggatagctg tcttcttttc
     5341 cttcaagttc aagacttttt aaaagctcca gagccctttt tctaatattg tcaggactgt
     5401 catttttaag aaagaccggt gcttccatca agttttccat tacactcata tgaggaaaca
     5461 aattgaaatc ctggaatacc agtccgattt cctgccgtat tgcctgcagc tcgccctttt
     5521 gagcatactt tatctggctg ttttcttccc tgcacaaata catattattt attttaattg
     5581 accccttgtc tggagtttcc agcgatgtga ggcatcttaa gagggttgtt ttgccttcgc
     5641 cggatttgcc cctgatacat acaatttcac ctttatttac agaaaaggag atatcatgga
     5701 agaccatact tttgccgaag cttttgctta aattttctac tgttaaaacc ataaatattc
     5761 tcctatctgt agtaatcgta cctgttttcg attttatcaa gcacttttgt cagaattgca
     5821 attataacca ggtatacaag cccaatatag atgtaggggc tgaaagctgc ataaacattt
     5881 gatactgacc ttgcagcctt taaaatgtca ctggagccta aaatatatac cagggatgta
     5941 tcttttacca atgtaattac ttcatttccg attgagggca taacattttt tatgacctga
     6001 ggaagaataa ttttcataaa ggtatgggtt ttgcttaagc ccagaaccaa ggatgcttca
     6061 tgctggccaa tatctatgga attaattcct cctctgaaaa tctctgcaaa ataagcagta
     6121 taatttaaaa caaaagcaat gactatagcc tgtcctctgc tgaagggata accaatcaag
     6181 ggcacccggt agtatccgaa gaatatgaac ataagctgca ataatagggg agtacccctc
     6241 attatataaa tgtaaattcc ggtaattttt gaaattattt tattttttga catcctaagc
     6301 tttgcaacca ctatggctaa aggaagggac ataatcaggg taagagcaaa cactcccaag
     6361 gttaccttca gcccttccgt agccataggt acaagcatgg tcaatacttt tgattccatg
     6421 gtatcccttt aattttcagc aaaccatttt tcataaatct catcataggt tccgtcactt
     6481 ttcatttcgt ccattgcatc atttattttt ttcaataatt ccgtatcgtc ttttcttagg
     6541 cctataccgt attcttcatc accaaagtct tcttccagca ctttgtattt ttcctcacct
     6601 gcctgcttca tgtaatatct tgcgttaacc tcatctacca ctagcgcatc tgaacgtcct
     6661 gattccaagt cgctgaacgc ttcgttgttt gtggcaaact caaccagctc tccaaatgag
     6721 gcggctacat caggttcagc gtttattgca tccagggcgc ttgattcggc ctgcacggta
     6781 acatttttac ctgccaggtc agcctttgta acaatatccg agtcagccat tgttactatt
     6841 atttggctgt tttccagata aggtttggta aatgctactt tttcctgtct ttcggcagtt
     6901 attgtgtagc cgttccagat taaatcaata tttcctccgt ttaattcagt ttccttcata
     6961 gaccagtcaa taggctggaa cttcattgtt acaccgattc tttcagcaac ttcatttgcc
     7021 aggtccacat caaatcctac caaatttcct ttttcatctc tgaaacccat aggtgcaaat
     7081 gtgtcatcaa gtcccatgac taattcttca gtttcctttt gtgcttcctg agcacatccg
     7141 gcagccatta ctgctattaa aactaataat actgctgcta aaattgattt caatacactt
     7201 tttttcattt ttcttctcct tatttaatta atattatta
//
LOCUS       DAOPUT010000070         7205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_10364_length_7205_cov_17.397483, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000070 DAOPUT010000000
VERSION     DAOPUT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10364_length_7205_cov_17.397483"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3418)
                     /locus_tag="PKK75_06430"
     CDS             complement(<1..3418)
                     /locus_tag="PKK75_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83271.1"
                     /translation="MTAAIKKKVNIPDPATGPVGFTVSSFKAAIKGISTVLKKPKILF
                     TTLFISALQAALSYLKILMPSSFFVTIASFFTFAQGGMYAGLIGAAGGIVGKGIYAWF
                     INTLFFSGSKVKSKNEQNEKNEKKAKGKIGLFLAGTGIALIAYNFLTGNATLENSIIG
                     ITALAACVRAIKKKNGFLIGLICSFTKGRMSRNTAACVIKGMALGFLAGVLSSLKFTG
                     IICYIAGAIIFVIALILMMGRKRTVAAAAALVLIFSSALPAYSSLLPDSEEWMTPWEA
                     YGINKDVQSGYLELIQAMDASPPVSMELTLIAVGGGYIDESGKIENQQVLAIDGKSKT
                     FSIDSPDNPSYSQYKFSSVIDLILEDGGWYADTITSYSVNNSWTNFETEEGRYGDYKK
                     CYFPTIPLEPGKIYIAIPISFEYGDASHGKAYSGMELYALVDRVKREKSTESTSQVSG
                     VWELKKIDTYLIKWVNKSISETEKFTGQWTGIVAGGDEVSIGVSGNTMEFIRTRQFHD
                     GYDQINWKAVFGEPPSSIKAFETISISAEEEGTYYNSSDNSTIEYEASLACASFLKGL
                     RPEKFSSKEYDTDTMAYAFGAGMGQTWFERSEGFLQSSAPQGQIDGEILTLELALGNN
                     QPYDTGAGRPWEIPIISYQYEWKAGTIIPAGSQGEEDEDTESDGGWWSGTDDTDEEWS
                     EHASEEETMAIGAVSALAAIAGAGAAAAGSLGIGPGGGYGSFIKRNKDGDLVVRDPVT
                     GEENLYVGNKKTGLYTNPLTGAEYTEDELTSHVASRAENAGVLGQDYETAQDAIKQQR
                     KDNQELSQAESEVNLEKSMKKLQKDLETKGAIGDDYADELAKKLKILQSQKKIDGEFN
                     KDDLKKFQKTFNRWTRGEIAGSRGLPKAENDWEIYKQGLALSGEEIARGDSWKAIGLR
                     VAVSLLTGGASTGTAFVVEAGLELAQAGYTIKDYVDKGGDDWTEGAQLAITKTLVGEG
                     IGRSIGLGLKGLGLAASKTGQALSKTKAGKYIVDGISDAVEKTSRVLNANVGDVPKVI
                     KQISKEASESAAAAKKAASMAGKLKTSAANKAAQTADDAAKKAAQTADDAAKKTAQST
                     EDAAKKTAQSTEDAAKKAAPPKTVKDTDAYKQASRQVDDSLNKSKAAAAEK"
     gene            complement(3420..5288)
                     /locus_tag="PKK75_06435"
     CDS             complement(3420..5288)
                     /locus_tag="PKK75_06435"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNZ83272.1"
                     /translation="MKKTIMLCTALIISILTFVAVPAFGAEAFNITSYNIDMTVYEDN
                     SYAVIETIDVAFTEQRHGIIRSLPLKTNRGKAALISDIDVFDHKFSTEKISNNIEIKI
                     GDPDRHASFSEKYTIGYVYTIGDDYFDDRDELYWNLIGNDWDCTIENVTFKINMPKPF
                     TPDRLYFTHGKKGSTKHDGVDWSIEGNTIKGSLTVTLMPNDSLTIAMPLPEGYYSEAE
                     RISTDVPYGWLVPIASLAAGTGLWVLMGRKKQIFPTVEFYPPQGVTSADAGFLIDGRV
                     DSFDITSLIIYWADKGYLSITEETVKKPFGTKKSFTLTKLRDIPDDSQDYEKEMFEGL
                     FFMGDGENVRTDQLENRFYIVGERVKKQIKSSFEDNEETRIFLRTNILCRILLLLTGV
                     ISTLPFTYTAMKESGNVGAAGLLLKTIIMSTAIMAAFYFLMHILKNWKNIKKGEKFIQ
                     LFFAFVFCGAVCGTFIYSLSLSGNVLTGLLSLAAAALLGIMSNLCSRRTKAGMWYQEK
                     LIGFREFIKAAELDRLKLLVDENPNYFYNVLPFAMVLGVTDKWGKNFDSIMKEPPDWY
                     HSSSMQGRFSAMSFATGLDNDLKIFSSGIASRPSSSSSVSGGSSGGGSGGGGGRSW"
     gene            complement(5303..5854)
                     /locus_tag="PKK75_06440"
     CDS             complement(5303..5854)
                     /locus_tag="PKK75_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNZ83273.1"
                     /translation="MEVFIGLLIILVLLAVLTVMSYNGFVKSRNQIEEAFSTMDVYLK
                     KRYEMIPRLVEIVKGYSIHEKDTLEKIVQARSRAINSGSVEERGQNENMLSGAIKSLF
                     AISEGYPELKANENYLELQNQLSDLEDEISQSRKYYNGVVKIMNNKVEIFPSNLFAKV
                     FGFNRYPYFVADEYERQNVEIRF"
     gene            complement(5881..6420)
                     /locus_tag="PKK75_06445"
     CDS             complement(5881..6420)
                     /locus_tag="PKK75_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNZ83274.1"
                     /translation="MFCENCGTQVADDSAFCQECGSKVTFTKNETQINEESINISKKP
                     GSQGQRGPVPPVMRQTALRQPAPKQPSEPYGREPSSGQAPKQPSEPYRKEASPRQPSE
                     SYMGKAVDIIGIGQYMLMIFLACIPIINIILLVKWGFFIKQKPNKSNFAKALLIYNII
                     SVIIYVLYIRLWLSVTSGI"
     gene            complement(6501..>7205)
                     /locus_tag="PKK75_06450"
     CDS             complement(6501..>7205)
                     /locus_tag="PKK75_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83275.1"
                     /translation="GYGSFFCWNCGKGIERGEKNCPHCGSIYSGDNRYGNVSALGAGG
                     VGWSNNTTHPCFKQYFKNNRNYGLIWLIGISILVPAVLVLTDEIEFNTEGIMVIGGVL
                     AVFWITGLIFLFKNRGDKTAWDGIVEDKKIFQKNRRKKSPEGKKYEESYTEYIVYIRR
                     QNKEIIELKEEDRAKYDYFNIGDYVHYHGDKWLHYIEKYDKSLDTTIFCACCGNISDI
                     RDNYCERCGCILLKAD"
BASE COUNT         1858 a         1671 c         1221 g         2455 t
ORIGIN      
        1 ttttctcagc tgcagcagct ttggatttat ttaaactatc gtcaacctgc ctggacgcct
       61 gcttgtatgc atctgtatcc ttgacagtct ttggtggtgc agcttttttg gctgcgtcct
      121 cggtgctttg agcggttttt ttggctgcgt cctcagtgct ttgagcggtt tttttagctg
      181 cgtcatcagc tgtttgggct gcttttttgg ctgcgtcatc agctgtttgg gcagctttgt
      241 tggcagctga tgtttttagt tttcctgcca ttgaggctgc ttttttcgct gcagcagcag
      301 attcgcttgc ttccttgctg atttgcttta ttacctttgg cacatctcct acattggcat
      361 ttagtactct tgaagttttt tctactgcat ctgaaattcc atcaacaata tactttcctg
      421 cttttgtttt agataatgct tgccctgtct ttgacgctgc aagaccaagg ccttttaatc
      481 ccaaacctat agaccttcct ataccttcgc ccaccaaagt ctttgtgatt gcaagctgtg
      541 ctccttctgt ccagtcatct ccacccttgt caacatagtc ctttattgta taacctgctt
      601 gagcaagctc caaacccgct tctacaacaa aagcagttcc ggttgaagca ccgcctgtca
      661 aaaggcttac agctacgcga agtcctatgg ctttccagct gtctcccctt gctatctctt
      721 ctcctgataa tgccaatcct tgcttgtaaa tttcccaatc gttttctgct tttggcagtc
      781 ctctgcttcc tgcaatttct cctcttgtcc atctgttaaa tgttttctgg aattttttca
      841 aatcatcctt attaaactca ccgtcgattt ttttctgact ttgaagtatt ttaagcttct
      901 tcgctaactc atctgcataa tcgtcaccga tggctccttt ggtttccaaa tctttttgca
      961 gttttttcat gcttttttca agatttactt cactttctgc ctgacttaat tcttgattat
     1021 ccttacgctg ctgttttatt gcatcttgtg cagtttcata atcctgacct aatacacctg
     1081 cattttctgc ccgtgaagct acatgacttg ttagctcatc ttctgtgtat tcagctcctg
     1141 ttaatggatt tgtatagagg cctgtttttt tgttgcctac atagaggttt tcttcaccgg
     1201 tgacggggtc tcttacaaca aggtccccat ccttgttcct ttttataaat gaaccataac
     1261 ctccccccgg acctattcct aaacttcctg ctgctgccgc ccctgcacct gcaatagcag
     1321 caagagcaga cacagcccct atagccattg tctcttcttc gcttgcatgt tcgctccact
     1381 cttcatctgt atcatccgtt ccgctccacc agccgccatc actttctgta tcttcatctt
     1441 cttcaccttg gcttccggca ggaattatgg tccctgcctt ccactcatat tggtatgaaa
     1501 taattggtat ttcccatggt cttcctgcac ctgtatcgta tggctgatta ttccctaatg
     1561 caagttcaag tgtcaaaatt tctccatcta tctgcccctg aggtgctgat gactgaagga
     1621 atccctctga cctttcaaac caagtctgtc ccatgcctgc accgaatgca tatgccatag
     1681 tatcagtatc atactctttg ctactaaatt tttcaggcct taatcctttt aaaaatgaag
     1741 cacatgcaag acttgcttca tattctattg ttgaattgtc agaactatta taataagtgc
     1801 cttcttcctc tgctgatata ctaatagttt caaatgcttt tatggaagaa ggtggctcac
     1861 caaatacagc tttccagtta atttggtcat atccatcatg gaactgtcta gtacggataa
     1921 attccatggt atttcctgat accccaatac ttacttcatc tccacctgct acaattcctg
     1981 tccactgacc ggtaaacttc tctgtttcac ttatgctttt gttaacccat tttataaggt
     2041 aagtatcaat tttcttcagc tcccaaactc ccgatacttg gctggtgctt tccgtactct
     2101 tttctctttt tacacggtca acaagcgcat ataattccat tccgctgtaa gccttaccat
     2161 gacttgcatc tccgtattcg aatgaaatgg gaattgcaat atatatcttt cctggctcta
     2221 aaggtatagt tggaaaatag cattttttat agtcaccata ccttccctct tctgtttcga
     2281 aatttgtcca agagttattg actgaataag atgttattgt atcggcatac catcccccat
     2341 cttctaagat caaatctatg acacttgaga atttatattg agaatatgaa ggattgtcgg
     2401 ggctgtctat gctaaaagtt ttgcttttac catcaatggc taaaacctgt tgattttcta
     2461 tttttcctga ttcatcaata taacctcctc caacagcaat aagagtaagc tccattgaaa
     2521 caggcggcga agcatccatt gcctgaataa gctctaaata acccgactgt acatctttat
     2581 tgatgccata agcttcccat ggagtcatcc actcctcaga atcagggagc aaggaagagt
     2641 aagcaggaag cgcagagcta aaaatcaaaa ctaatgccgc agctgctgca acagttcttt
     2701 tcctgcccat catgagaatt aatgctataa caaatattat tgctcctgct atgtaacaaa
     2761 ttatcccagt aaattttagt gatgacaaaa cacccgccaa gaatcccaag gccattccct
     2821 taataacaca ggctgctgta tttcttgaca ttcgtccctt tgtaaatgag catattaaac
     2881 cgattaaaaa tccgtttttt ttcttaatcg ctcttacaca ggctgcaaga gctgttattc
     2941 cgataatgct gttttccaag gtcgcattgc cggtcaaaaa attatatgca ataagggcta
     3001 tgcctgttcc tgccaagaaa agaccaatct ttccctttgc ctttttctca tttttttcat
     3061 tttgctcatt tttgctttta accttggagc ctgaaaaaaa cagtgtattt ataaaccatg
     3121 cgtatattcc ttttcctaca attcctcctg cagctccaat aagacctgca tacattcccc
     3181 cttgagcaaa tgtaaagaag cttgctattg taacaaaaaa agatgagggc ataagaattt
     3241 tcaggtagga taatgctgcc tgaagtgcag atataaataa agttgtaaat aaaatttttg
     3301 gctttttaag taccgtgctt atgcctttaa ttgctgcttt aaagcttgac actgtaaacc
     3361 cgacgggtcc cgtggccggg tcgggaatat taactttctt ttttattgct gcagtcattt
     3421 taccagctcc ttccgcctcc gcctccagac cctcctcccg agcttcctcc gctgacgctt
     3481 gaactgctcg atggtcttga ggctatgccc gatgaaaata tttttaaatc attatctaaa
     3541 cctgttgcaa agctcattgc gctgaacctg ccctgcatgg atgatgaatg ataccagtcc
     3601 ggcggttctt tcattatgct gtcaaagttt ttcccccatt tatctgtaac tccaaggacc
     3661 attgcaaagg gtaatacatt ataaaagtag ttgggatttt catcaactaa aagctttaat
     3721 ctgtctaatt ctgcagcctt gatgaattcc ctgaatccta taagtttttc ctggtaccac
     3781 atgcctgctt tggtccgccg gctgcatagg tttgacataa taccaagcaa cgctgctgct
     3841 gctaaagaaa gtaaacccgt taacacattt ccggataagc ttaatgaata tataaaagtg
     3901 ccgcaaactg ctccgcaaaa tacgaaagca aaaaacaatt gtataaactt ttcccctttt
     3961 tttatattct tccaattctt cagaatatgc attaagaaat aaaaggctgc cattatagct
     4021 gtagacatta tgatggtctt taacagcagt cctgctgcac caacattgcc tgattctttc
     4081 attgctgtat aggtaaacgg cagtgtactg attactcctg tcagcagaag caaaatgcgg
     4141 cacaatatgt ttgttcttaa aaagatacgg gtttcttcat tatcttcaaa gcttgattta
     4201 atctgctttt ttactctttc accaacaatg taaaaacggt tttccaattg gtcagtcctt
     4261 acattttctc cgtcccccat gaagaacagg ccttcgaaca tttccttttc atagtcctgg
     4321 ctgtcatcag gaatatctct aagttttgta agtgtaaaag actttttagt gccaaagggc
     4381 ttttttacgg tctcttcagt gattgaaaga tatcccttgt ccgcccagta aataattagt
     4441 gatgttatat cgaaggaatc taccctgccg tcgattaaaa atcctgcatc tgcactggtc
     4501 actccttgag gcggataaaa ctcaacagtt ggaaatatct gctttttccg tcccatgagt
     4561 acccacagtc ctgtgcctgc agccaatgag gcaattggga ccagccatcc gtaaggcaca
     4621 tcagtgctta ttctttctgc ctctgaataa tatccttccg gaagtggcat agcgattgta
     4681 agggaatcat taggcataag agtgaccgtc aatgacccct ttatggtatt tccctcaata
     4741 ctccagtcca caccgtcatg cttagtgctt cccttttttc catgtgtgaa atacagcctg
     4801 tcaggtgtaa aaggctttgg catattgatt ttgaaggtaa cgttttctat tgtacagtcc
     4861 cagtcattcc cgattaaatt ccagtagagt tcatctctgt catcaaaata gtcgtctcct
     4921 atggtataga catagccgat tgtatatttt tcagaaaaag atgcatgtct gtcagggtct
     4981 cctattttta tttcaatatt atttgatatt ttctcagtag aaaatttatg gtcaaaaaca
     5041 tcaatatctg aaatcaacgc ggcctttccc ctgtttgttt tcaatggcag acttctgatg
     5101 atgccgtgcc tttgctcagt aaaggccaca tcgatggttt caattacggc ataggagtta
     5161 tcctcataaa ccgtcatatc aatattgtaa gaagtaatat tgaatgcttc agctccgaag
     5221 gcaggtaccg ccacaaatgt taaaatggag atgattaatg cagtacaaag cattatagtt
     5281 tttttcattg ctcttcccct gattaaaatc taatttctac attttggcgc tcatattcat
     5341 cagccacaaa atatggatat ctattgaatc caaaaacttt tgcaaataaa tttgaaggaa
     5401 atatttcaac cttgttgttc ataattttaa caacaccatt atagtatttg cgggactggg
     5461 atatttcatc ttccaggtca gaaagctggt tttgaagctc taaataattt tcgtttgctt
     5521 tcagttcagg atagccttcc gaaattgcaa aaagggattt aattgcaccg gacagcatat
     5581 tttcattttg ccctcgctct tccacagacc ctgagttaat tgccctgctc cttgcctgga
     5641 caattttctc aagagtatct ttttcatgta ttgaataccc ttttactatt tctaccagtc
     5701 ttggtatcat ttcataccgt ttttttaaat acacatccat ggtggaaaaa gcttcttcaa
     5761 tttgattcct cgactttaca aaaccgttgt atgacatcac cgtcaacact gccaataaga
     5821 ctaagatgat tagtaaacct ataaatactt ccatttttct tcccctttca aataatagtt
     5881 ttaaattcct gaagtgacac taagccataa ccgtatatat aaaacataaa ttattactga
     5941 aattatgtta taaataagca aggctttggc gaaattgctt ttgttgggtt tttgcttgat
     6001 aaaaaatccc cattttacca agagtattat attgataatc ggaatgcatg ccaagaaaat
     6061 cataagcata tactgcccga tacctataat atccacagct tttcccatgt aggattcaga
     6121 tggctgccgg ggtgatgctt ctttcctgta tggctctgat ggctgtttgg gtgcctgtcc
     6181 ggacgatggt tctctcccat agggttcaga cggttgtttg ggtgctggtt gccggagtgc
     6241 tgtttgtctc atgacgggag ggacaggtcc ccgctgcccc tgggagccgg gctttttact
     6301 tatgttgata ctttcttcat ttatttgcgt ttcatttttc gtaaatgtta ctttgctgcc
     6361 gcattcttga caaaatgcag aatcatcggc aacctgtgta ccgcaatttt cacaaaacat
     6421 aattttcctc cttattttgt catcatgatt ttatctttaa gacatgagat catccggcaa
     6481 gccacagaat ggcaagatgg tcaatctgct tttaataata tacaaccgca cctttcacaa
     6541 taattatccc gtatatcact aatgttgccg cagcatgcgc aaaaaattgt tgtatccaaa
     6601 gatttatcgt acttttcaat ataatgaagc catttgtctc cgtgatagtg cacataatcc
     6661 cctatattga aataatcata tttagccctg tcttcttcct ttaattcaat aatctccttg
     6721 ttctgtctgc gtatatagac aatatattct gtatatgatt cctcgtattt tttcccttca
     6781 ggactttttt tgcgtctgtt cttttgaaat atctttttat cttcaacgat gccgtcccag
     6841 gcagttttat ctccccgatt cttaaataaa aatataagac ctgtaatcca aaatacagct
     6901 aagacaccgc ctattaccat aatgccttcc gtattaaatt caatctcatc tgtgagaacc
     6961 aagactgcag gaactaatat gctgattcct atgagccaga tcagcccgta atttcgatta
     7021 tttttaaaat attgtttgaa acagggatga gttgtattat ttgaccagcc tacacctcct
     7081 gctcccaagg cagaaacatt tccatacctg ttgtctccgc tgtatatgga gccgcagtgc
     7141 gggcagtttt tttcccctct ttcaattcct ttaccgcaat tccagcaaaa aaagcttcca
     7201 taacc
//
LOCUS       DAOPUT010000071         7148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_10474_length_7148_cov_16.801776, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000071 DAOPUT010000000
VERSION     DAOPUT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10474_length_7148_cov_16.801776"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKK75_06455"
     CDS             complement(<1..560)
                     /locus_tag="PKK75_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ83276.1"
                     /translation="MLKDRVCIVTGAASGIGLAIAETFANEGAKVVMADVNEAKLQEE
                     SARIGAVYFKGDLSKRDDCKGLVEFAVEKYSKVDILANVAGIQTVSPIEDFPEDKWYF
                     MMDLMLTAPFLLTKYCWPYMKEQGWGRIINMGSIHGLIASEYKSAYISAKHGLGGLTK
                     TAAVEGGKYGITCNMICPSYVRTPLV"
     gene            complement(581..1234)
                     /locus_tag="PKK75_06460"
     CDS             complement(581..1234)
                     /locus_tag="PKK75_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HNZ83277.1"
                     /translation="MDDKCYIAKRVAKELKDGDVVNLGIGLPTLVANYIPEGIEITLQ
                     SENGFLGMGPAAEKGKEDPYLVNAGGIHVTINPGGAFFDSATSFLIIRGGHVDATVLG
                     ALEVDEKGNLANWMVPGKMVPGMGGAMDLVTGAKKVIVAMNHTAKGNHKILKECTLPL
                     TAVNAVDLIITEMGVMEVTNKGLLLTEINPAYTVEDVQNATGATLIISDKLKQMEPQ"
     gene            complement(1237..1890)
                     /locus_tag="PKK75_06465"
     CDS             complement(1237..1890)
                     /locus_tag="PKK75_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HNZ83278.1"
                     /translation="MNKVISLNQAVDMINDGMTIMVGGFLGCKNPFKIVDAIVEKGVK
                     DITLIANDSSFPEVGIGKLIVNKQVKKLIASHVGTNKETGNQMNSGEMQVELVPQGTL
                     AERIRAAGAGLGGILTPTGLGTIVAEGKTVVEVDGKEYLLEKPLKADIALIVGAKVDK
                     KGNVVYSKATRNFNPIMATAADIVIVEADEIVEVGEIDPDNVMTPGIFVDYIVGGNE"
     gene            complement(1905..3083)
                     /locus_tag="PKK75_06470"
     CDS             complement(1905..3083)
                     /locus_tag="PKK75_06470"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNZ83279.1"
                     /translation="MREVVIVSAVRTPIGSFGGSLKDVSAVKLGAVAAKEALNRANVK
                     PEMVDEVIFGNVLQAGIGQNLARQVSIAIGVPIEVPSMTINKVCGSGLKTVQLAAQAI
                     MCGEADVVLAGGAENMSQAPYIVPKARWGLRMGDGAIVDTMVADGLTDIFNNYHMGIT
                     AENIAEQWNITRQEQDEFAVRSQNRAEAAIKSGRFKDEIVPVLIPQRKGDPIVVDTDE
                     FPKFGATIEGMAKLKPAFKKDGTVTAGTSSGINDGAAAVLVMSKEKADELGIKPLATI
                     VSFASAGVDPTIMGYGPVPSSKKALAKANMTIEDIDLVEANEAFAAQSLSVVKDLGLD
                     PEKTNVNGGAIALGHPIGCSGTRILVTLIHELVKRDAHTGLATLCIGGGQGTTLIIKR
                     "
     gene            complement(3385..5088)
                     /locus_tag="PKK75_06475"
     CDS             complement(3385..5088)
                     /locus_tag="PKK75_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ83280.1"
                     /translation="MIKYEDFQNLIDSMYEGVLFIDNKNNRIVNRQFKRIFDIVGEDN
                     KSLAKVLTKYKLLNFTNHDSYAESEIMIGNKSITVESFCYRNLNIFQFDIILFKEATE
                     IQEARNEIEELKLALNSLKDVLDNAYQGFVLVNEEGIIIKWNYEKLFGIKEEDALGKH
                     VEDVIENTRLHVVVKTGKKELYDVQRIQGHDMIASRTPIFKDDKVIGAVGTVLFKDIK
                     EVTDLAKRLKILESTVDKYKSEISKMYHANYCFDDIITQNVHMLELIDIAKKAANSGS
                     TILIEGESGTGKEYFAHAIHEESNRRLAPFIRINCAAIPHELLESELFGYEAGAFTGA
                     KKEGKIGKLELANGGTVLLDEISSMPMSMQAKLLRVLEEREFERIGGNTSIKIDIRLI
                     ACTNEDIEHIVSKNKFRQDLYYRLNVVKIKIPPLRERLDDIPCLCDNILNKQIQSVGL
                     TSKTVTDRAIFAMKLYNWPGNVRELRNILERAANVSIGNYIGIQHLPDFINEKLNDDV
                     KKHDVQTLKYKLAEAEINAIVEALKYSNGNRTVAANLLGIHRTALYKKLEGYGIDIKT
                     I"
     gene            complement(5231..5908)
                     /locus_tag="PKK75_06480"
     CDS             complement(5231..5908)
                     /locus_tag="PKK75_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNZ83281.1"
                     /translation="MAILERFGDIIKANINVLLEKMEDPEKMIDQYLEDMMEDLAEVK
                     QSTAEVIAEETRTKRLVDQNEAEVAKYNEFAKKALVAGNEGDARTFINKKQELENVGA
                     GLMTAYAAAHENAIKMRQMHDKLTADIEALKARRQVIKSKVKVAKTQETLNEAAQATK
                     KISGAKSAFERMEDKASKMLDEANAMADLNMEPIDEAKALEEKYAAMGNASVDEELNK
                     LKEELGL"
     gene            complement(5943..7037)
                     /locus_tag="PKK75_06485"
     CDS             complement(5943..7037)
                     /locus_tag="PKK75_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNZ83282.1"
                     /translation="MARGGGSGGRSGGGSFGGSRGGGGRSGGFGGGFSIGGRGGSSGR
                     SGGSFGRSGGSFGSSGGSSGTGGGIFGGSRSTGPHIGPIFAPRVPMGGGFGGGYSPRR
                     SSGGGGCGCALLIIVVIVLAIILIIGFSMLNSPNTKSSDSIITPSSVERIPLPSGSVN
                     ETGYYTDELGWIINETKLIDGMKHFYKETGVQPYLYLTDNVNGSYYPTQFEIESFAES
                     LYDELFTDEAHLLFVFFEYEGMYRDWYIAGTQAKSVIDDEAGNILLDYIDRYYYDDNL
                     SDDEFFSKSFKDAADRIMKVTRSPWITVFIVIGLVVLAFVLFLWWKKHMEQKNLEAKR
                     REEILNTPLDRFGDAETEELTKKYQDDNEQ"
BASE COUNT         2022 a         1566 c          961 g         2599 t
ORIGIN      
        1 tcaaccaatg gtgttctaac atatgaagga caaatcatat tacaagtaat accatacttt
       61 ccgccttcaa cagctgcagt tttagttaat ccgccaagac catgtttagc tgatatataa
      121 gcagatttat attctgaagc aataagtcca tgaattgaac ccatattgat aattcttccc
      181 cagccttgtt ctttcatata aggccagcaa tattttgtta acaagaacgg agcagttaac
      241 attaaatcca tcataaagta ccatttgtct tcagggaaat cttcaattgg actaacagtt
      301 tgaataccgg caacattagc caatatgtca acctttgaat atttctctac ggcaaattca
      361 actaaacctt tgcaatcatc acgtttgctt aaatctcctt taaaatatac agcaccgatt
      421 cttgcagatt cttcttgcaa ttttgcttcg ttaacatccg ccataacaac ttttgcacct
      481 tcatttgcaa aagtttcagc tattgccaag cctataccgc ttgctgctcc tgttacaata
      541 catactctgt cttttaacat tttaaatcct ccattttttt ttactgtggt tccatttgtt
      601 ttaatttatc agaaattatt aaagtagctc ctgtagcatt ttgaacatct tcaactgtat
      661 aagccggatt tatttcagtt aataataaac ctttgtttgt tacttccata actcccattt
      721 cagtaattat taagtcaaca gcatttacag ctgttaaagg aagtgtacat tcctttaata
      781 ttttatgatt tcctttagct gtatggttca tggctacaat aacctttttt gcacctgtta
      841 caaggtccat tgctccaccc attccgggaa ccattttgcc cggtaccatc cagtttgcaa
      901 gatttccttt ttcatcaact tccaaggcac ctaaaactgt agcatctacg tgccctcctc
      961 ttatgattaa gaaggatgta gcgctatcaa agaaagcacc tccaggatta attgttacat
     1021 gaattcctcc tgcatttact aaataaggat cttcttttcc tttttctgca gcaggtccca
     1081 ttcccaaaaa tccattttca gactgcaatg taatttcgat accttcaggt atgtagttag
     1141 caaccaatgt aggtaatcct attcctaaat ttacaacatc gccgtctttt aattctttgg
     1201 caactctttt agctatataa catttatcat ccatcattat tcattacctc ctactatata
     1261 atctacaaat atacccggag tcatgacatt atctggatca atttcaccca cttcaacaat
     1321 ttcatctgcc tcaacaataa ctatatcagc agcggttgcc attatgggat taaaatttct
     1381 tgtagcctta gaatatacta cattgccttt tttatcaacc ttagctccta ctataagagc
     1441 gatgtcagct tttaaaggtt tttccaacag atattctttt ccgtctactt ctacaactgt
     1501 ttttccttcg gcaactattg tacctaaacc tgtaggagtt aaaattccgc caagtcctgc
     1561 acctgctgct cttattcttt cagccaaagt accttgtgga actaattcaa cttgcatttc
     1621 accggaattc atttgatttc ctgtttcttt gtttgttcca acatgagaag caattaattt
     1681 ttttacttgt ttgttaacaa taagctttcc aataccaact tccgggaaag aactatcatt
     1741 agcaattaaa gtaatatcct taactccctt ttcaacgata gcatcaacta ttttaaaagg
     1801 gtttttgcat cctaaaaatc cgccaaccat gatagtcata ccatcgttaa tcatatcaac
     1861 agcttgattc agagaaatta ccttattcaa tttgtatcct ccttttatct tttgatgatc
     1921 agtgttgtac cttgtcctcc gcctatacat aacgttgcca agcctgtatg ggcatctctt
     1981 tttaccaact catggattaa tgtaacaagt atcctcgttc cagaacatcc aattgggtgt
     2041 cctaaagcta tagctcctcc gttaacatta gttttttccg ggtctaatcc caaatcttta
     2101 actacagata aggactgagc tgcaaaagct tcattagctt ctactaagtc aatgtcttct
     2161 atagtcatgt tagctttagc taatgctttc ttagatgaag gaacaggtcc gtatcccatt
     2221 attgttggat caactcctgc agatgcaaat gatacaattg tagccaaagg tttaattcca
     2281 agctcatctg ccttttcctt tgacataaca agtactgctg ctgcgccgtc atttatacct
     2341 gatgaagtgc cagctgttac agttccatcc tttttaaaag caggttttaa ttttgccata
     2401 ccttctatgg tagcgccaaa tttaggaaat tcatcagtat ctactactat tgggtcgcct
     2461 tttctttgag gtattaaaac aggaacaatt tcatccttaa atcttccgct cttaattgct
     2521 gcttctgctc tgttttggct tcttactgca aattcatctt gttcttgtct ggttatgttc
     2581 cactgctcgg caatgttttc tgctgtaatt cccatatgat aattattgaa aatatcagtc
     2641 aatccgtctg caaccatagt atcaactata gcgccatcac ccattcttaa tccccatctt
     2701 gctttaggaa caatatatgg cgcctggctc atattttctg caccacccgc aagaacaacg
     2761 tctgcctcac cgcacattat tgcctgagca gcaagttgaa cggtttttaa tcctgaaccg
     2821 caaactttat tgattgtcat agaaggaact tcgatcggta cgcctatagc tatagacact
     2881 tgtcttgcta aattttgtcc aattcctgct tgaagaacat tcccgaaaat aacttcgtca
     2941 accatttcag gcttaacatt tgctctatta agtgcttctt tagctgcaac agcaccaagc
     3001 ttaacagctg atacatcttt gagacttcct ccaaagcttc ctataggcgt tcttacagca
     3061 ctaacaataa caacttctct catcactgac ctcctttatg tattatggaa taatttacca
     3121 taattattca tatatttata agcaataagc atgccaactg cttgtcttcg tttaaattat
     3181 tttatttgtt gaaatatcaa tgttttttca atataaaatc tttcttttta aaaatttttt
     3241 tagtaaaaaa tataatatgt agaaaaattt ctacatattt tgaattgaac aaaatattaa
     3301 tctaaaatta tgcttaattt ccataaaaat tatacttgta gattattttc tacattattt
     3361 tacaatgtag aaaataatct acatttaaat agtttttata tcaatgccat atccttcaag
     3421 tttcttatat aatgctgttc tatgaatacc taacagattt gctgccacgg ttctgtttcc
     3481 attactatat tttaatgctt caacaatagc atttatttct gcctcagcca atttgtattt
     3541 taatgtttgc acatcgtgtt ttttaacatc atcatttaat ttttcattta taaaatccgg
     3601 taaatgctga atacctatat aattgccaat tgaaacatta gcagcccttt caagaatatt
     3661 tctaagttct cttacgttgc caggccaatt atataatttc attgcaaaaa ttgctctgtc
     3721 agttacggtt ttagaagtta agccaacgct ttgaatttgt ttatttaaaa tattatcaca
     3781 taagcaagga atgtcatcta acctttccct caatggaggt atctttattt taacaacgtt
     3841 aagtctataa tataagtcct gtctaaattt atttttactt acaatgtgtt caatatcctc
     3901 attagtacaa gcaataagcc taatgtcaat ttttattgaa gtattgccgc ctattctttc
     3961 aaattctctt tcttccaata cccttaacaa ttttgcctgc atgctcatag gcatagagct
     4021 tatttcatcc aaaagcaccg ttccgccgtt tgcaagttct aactttccta ttttaccttc
     4081 ttttttagct cctgtaaagg ctcctgcttc atatccgaac agttctgact ccaataactc
     4141 atgaggtatt gcagcacaat ttattctaat gaagggggct aaccttctat tgctttcttc
     4201 atgaattgca tgggcaaaat attccttgcc tgtcccgctt tcaccttcaa ttaatatagt
     4261 tgagcctgaa ttggctgctt ttttcgcaat atcaataagt tcaagcatat ggacattttg
     4321 tgtaattata tcatcaaaac aataattggc atgatacatt ttgctgattt cacttttata
     4381 cttatcaacg gtgctttcaa gaattttaag tctttttgcc aagtcagtaa cttctttaat
     4441 atctttaaac aaaaccgttc caacagcacc aataacttta tcatctttaa aaattggagt
     4501 cctgctggca atcatatcat gcccttgaat tctttgaaca tcatacaatt cctttttacc
     4561 tgttttaaca acaacatgaa ggcgtgtatt ttctattaca tcttccacat gctttcctaa
     4621 tgcatcttct tctttaatac caaaaagctt ttcataattc catttaatta ttataccttc
     4681 ttcattaact aaaacaaacc cttgataagc attgtccaac acgtctttta atgaatttaa
     4741 cgctaatttc aattcttcta tttcatttct tgcttcttga atttcagttg cttccttaaa
     4801 caaaataatg tcaaattgaa aaatattcag attacggtaa caaaatgatt ctactgtaat
     4861 acttttatta ccaatcataa tttcactttc agcataacta tcatgatttg taaaatttaa
     4921 caatttgtat tttgtaagaa cttttgctaa tgacttattg tcctcgccca ctatatcaaa
     4981 aattctttta aactgtctgt taactattct attgttttta ttatcaatga ataatactcc
     5041 ttcatacatt gaatcaatta aattttgaaa atcttcatat tttatcataa atatatcctt
     5101 agtttaatta taaaagtaaa ataatatact aatatattaa taaatttaaa tgtttatgtc
     5161 aactgtttgt gtcgatttga gttaaagacc gacttaaaaa actgccgttt ggcagttttt
     5221 ttggaagtct ttataaacct aattcttctt ttaatttatt caattcttca tctactgatg
     5281 cattgcccat tgctgcatat ttttcttcta aagcttttgc ttcatctata ggctccatat
     5341 ttaggtcagc catagcattt gcttcatcca gcatcttgct tgccttatct tccattcttt
     5401 caaaagcgct ttttgcaccg gatattttct ttgttgcctg agctgcttca tttaatgttt
     5461 cctgggtttt agcaacttta acctttgatt taataacctg gcgccttgct tttaatgctt
     5521 ctatgtcagc ggttaatttg tcatgcatct ggcgcatttt aattgcattt tcatgagcag
     5581 cggcataagc tgtcataagt cctgcaccta cattttccaa ctcttgtttt ttgttgataa
     5641 atgttcttgc gtctccctca tttcctgcta ctaaggcttt ctttgcaaat tcattgtatt
     5701 ttgcaacttc tgcttcattt tgatcgacta atctctttgt gcgcgtttct tctgcaataa
     5761 cttctgctgt gctttgtttg acttcggcaa gatcctccat catgtcttcc aaatattggt
     5821 caatcatttt ttccggatct tccatttttt caagcaatac gtttatgttt gccttgataa
     5881 tatcaccaaa tctttctaat atagccatct tttcctcctt attattatct gccggtggca
     5941 gattattgct cattatcatc ctgatacttt ttagtaagtt cttcagtctc tgcatctcca
     6001 aacctatcaa ggggagtatt taatatttct tctcttcttt tagcttccag atttttttgt
     6061 tccatatgtt ttttccacca taggaagagt acaaaagcta aaacaacaag ccctattaca
     6121 ataaacactg taatccatgg agacctggta accttcataa tcctgtcagc tgcatctttg
     6181 aaggacttgc tgaaaaattc atcatcgctt aaattgtcat cataatagta tctgtctatg
     6241 taatcaagca ggatattgcc ggcctcatca tcaattacac ttttcgcctg ggttccggca
     6301 atataccaat ccctgtacat gccttcatat tcgaagaata caaataataa atgtgcttca
     6361 tccgtaaaca gctcatcata taagctttct gcaaaggatt ctatttcaaa ttgggtcgga
     6421 taataggaac cgttaacatt atctgtcaaa tataagtatg gctggactcc tgtttcctta
     6481 taaaaatgtt tcatcccgtc aatcagcttt gtttcattta ttatccagcc caactcatct
     6541 gtgtagtagc ctgtttcatt aacagatccg gacggaagcg gaattctttc aacagatgag
     6601 ggggtaatta tactgtcgct gctttttgta ttaggagaat taagcattga aaaaccaata
     6661 attaaaataa tagcaagcac tattacaaca ataattaata gagcgcatcc acagccaccg
     6721 cctccggaag acctccttgg gctgtatccg cctccgaatc ctcctcccat gggcaccctg
     6781 ggagcaaata tgggtccaat atgggggcct gtgcttctgc tgcctccaaa aatcccgccg
     6841 cctgtgccgc ttgaacctcc gctgcttcca aatgagccgc cgctgcgtcc gaatgaaccg
     6901 ccgcttctgc cgcttgaacc tccgcggcct ccaattgaaa atccgcctcc aaaaccaccg
     6961 cttcgtccgc cgcctcctct gcttccgcca aaggaaccgc cgccgcttct gcctcctgaa
     7021 cctccgcctc ttgccaatta aatccctcca attaaatgta tggaaattta tattttgtca
     7081 attatatttt atacccttat ttaaatattt caaacaataa ttatcctttc acttaattat
     7141 atcataat
//
LOCUS       DAOPUT010000072         7138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_10491_length_7138_cov_19.196245, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000072 DAOPUT010000000
VERSION     DAOPUT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10491_length_7138_cov_19.196245"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1420
                     /locus_tag="PKK75_06490"
     CDS             230..1420
                     /locus_tag="PKK75_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNZ83283.1"
                     /translation="MNKRIKRWLPVILVLIFAISSVTPALAAPRIPDFVRLPDQAPAV
                     INYVALGDSNAAGVRAMSGELPGWEEGSDCGYTDLIAKWLFDEGFIDSFNEDYSISGN
                     TAAKLARATSSKAYKNLVHADIVTLTIGANDFLEPFYAYFDSCTENRVPIDPVEALLA
                     LKAGVEYFDAGGGKEELQANVETILRKTLKASKDVKVYVMGYYNPLPILIELAEQGIV
                     FDLGGVSADISLDDLAFGFSNMGVLGMDYEVFVSIPPEKKLFHLNSILDQLLPTLLPS
                     LSPELQELLLAASGVLSIIDLAPQVMKLNDVLEAAIDAVEAKYPDSIEFIETLPIVNG
                     YSEGLYKRPGGHMFAYGFGYLYADISNPLVPMADIHLTEDGYAAVAALFEAEIDSDFG
                     LSIP"
     gene            1664..>2043
                     /locus_tag="PKK75_06495"
     CDS             1664..>2043
                     /locus_tag="PKK75_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNZ83284.1"
                     /translation="MQLKQKKLNLNKIDDDIKILLLNEINNHILLDEQPSIFLNSIVN
                     TKIFKEHPFYMLHKLKETEQSPVHHPEGNAWNHTMLVVDQAAGLRHISRDEKAFMWAA
                     LLHDIGKAETTKNKRGKITAYNHES"
     assembly_gap    2044..2143
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2241..2846
                     /locus_tag="PKK75_06500"
     CDS             2241..2846
                     /locus_tag="PKK75_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNZ83285.1"
                     /translation="MSNVTAFLSYVIITTFTPGPNNIMAMSNATRYGFKKSIRFNAGV
                     FFGFLILMVLSSFLSVTLYSLIPSIKPVMTFIGASYILWLAWKIYKSKPHAEDESHAT
                     NTFTAGILLQFVNVKAIIYCITIVSTFIVPYYDSVFVLSIFSLFLASVSFLSTCSWAV
                     FGFLFQKFLFKNERIVNIIMALLLVYTALSFYLWGGVGMML"
     gene            2936..3679
                     /locus_tag="PKK75_06505"
     CDS             2936..3679
                     /locus_tag="PKK75_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HNZ83286.1"
                     /translation="MWVRAELKFKAKEVLKKNYWKSFLISLVLLLSGGDSSSSGGSAG
                     SNRERISEWIDFSEYTIIIVAVIILVIAFRILIGYALETGSRRFFVKLAQFKDDTGCY
                     NFAFDTLNFKGIISTMLLRDIYTFLWTLLLIIPGIIKSYAYRMTPYILADNPNLGADN
                     AITLSRKMMDGNKFELFILQLSFLGWYLLGLLAFGIGILFVNPYYNATEAQLYLVLRD
                     KAIDLGYCTGEDLNLNNDFMRHEGYYWEE"
     gene            3731..4282
                     /gene="yyaC"
                     /locus_tag="PKK75_06510"
     CDS             3731..4282
                     /gene="yyaC"
                     /locus_tag="PKK75_06510"
                     /inference="COORDINATES: protein motif:HMM:NF018564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HNZ83287.1"
                     /translation="MDPIEVHYKNSFAAIKIGDFLAEHMNNAAIVCIGTDKCIIDSLG
                     PLTGTLLMKENIKTDIYGTLEKPVHAMNINEYIKNLKKKKYDCIIAVDACLSNKKNQG
                     IIEVREGSITPGKGIGKFLPEIGDISIIGIVDSSDKEFHDLVQDTRLSLIYEMAEIIC
                     GGVLNACSMNISESYNQASISSF"
     gene            complement(4322..5689)
                     /locus_tag="PKK75_06515"
     CDS             complement(4322..5689)
                     /locus_tag="PKK75_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ83288.1"
                     /translation="MKKRISLFLAVLMVILSFTPAWAVEIDEEKFSQDVEFMKKVIYF
                     VLDKYQYQVTEEDIMNGLYDGFFSVLDDYSTYYTPKEYQALLEDTAGEFSGIGVQIMD
                     INGQVVVVTPLPGSPAIEAGIKAGDIIKTVDGYEITGLTSSQASLLIRGEEGTPVKIG
                     ILRGRESLAFDLIRSTIVTNNIEVKILDDNIGYMKVTGFSENTAELVEKELKLFDANN
                     VEKIVIDMRNNGGGTLQSAVDLLNFFVTEGPVVYVDYAAGREDVYESTLKEQKYEIAV
                     LINEGSASATEIFAGAVKYKNEGIIVGTKSYGKGVVQSLYPLIDGSGVKFTTAEYFSI
                     DKTPVHKIGITPDIIVENGKIDISKYPRFSKEQKPQLGNVSLDVLAAEMILAELGYEI
                     NKPDGVYDQVSFEKLKKFQGDNLLYPYGTIDFTTQDALYNALIKHSESTLRDLQLEAA
                     MEALK"
     gene            complement(5727..7004)
                     /locus_tag="PKK75_06520"
     CDS             complement(5727..7004)
                     /locus_tag="PKK75_06520"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNZ83289.1"
                     /translation="MKTNLLTDILKNQVTPALGCTEPGAVAYGVAIAKEILGSEVKKL
                     NIEVDKNILKNGFSVGIPGTNEHGIIFAAALSLVIGKSEYLLEVLKDVDDNSIEKAIE
                     IVDSNIIDLKLKPNTHGLYIKAEAVGDKDTAEVIIRDSHTNIIFERKNEQVLFEKADS
                     SDTLEKKPKNEIKFEIKKFAIDELIDYADRVPLDEISFIQKGIDMNLKIADAGLNEGQ
                     GVGLGNYMYKKAGDVFTMAKAYTAAASEARMSGYLLPVMSSAGSGNHGLVAVIPISYI
                     GREQNVPNEKILRSVALSHLITIYVKVYLGALSPVCGCGVAAGVGCAAGLTYMLDGDK
                     EQIKGSINNMIAGVSGMLCDGAKLGCAYKLSISVDAAVDAAKMALENIYIPEDNGILG
                     SSAESSIQNLARVSNLGMDNTDEVILDVIINKC"
BASE COUNT         2072 a         1342 c         1350 g         2274 t
ORIGIN      
        1 cctttattat tacaataata taaacattat tgccctataa ttatatcata aaagccgtac
       61 tataaaaaga aatataaatt tttgtttgtt acatagtgtt attatgtgtt attaagtgct
      121 gataccctca agtcctaatt ggcttttgac atcctcaagc attttgttta aaaagtggtt
      181 taatattcgg aaatatgggt aaatattata tacgatagga ggaattaaaa tgaacaaacg
      241 aattaaaaga tggttgcctg ttattctggt gcttattttt gccatttcgt ctgtaactcc
      301 ggcgttagcg gcaccaagaa tacctgattt tgtacgtttg cccgatcagg cccctgctgt
      361 cattaattat gtcgccttag gagattccaa tgcggctggt gtgagagcga tgtcaggaga
      421 actccccggt tgggaagagg gctcggattg cggttatact gatctgatag caaaatggct
      481 ttttgatgaa ggttttattg attcatttaa tgaagactac agcatttccg gcaatacagc
      541 agcaaagctg gctagagcta cctcatcaaa agcttataaa aatttagtcc atgctgatat
      601 tgttacacta acaatcggag cgaatgattt ccttgaacct ttttatgcat attttgatag
      661 ttgtacagag aatcgtgttc ccatagatcc tgtggaggct ctgctcgcat tgaaagcggg
      721 tgtcgaatat ttcgatgccg gtggaggcaa agaggaactg caggcaaatg tggaaactat
      781 attaaggaaa acacttaagg caagtaaaga tgtaaaggtc tatgtaatgg gctattataa
      841 tcccttgccg atcttgattg aactcgctga gcaggggatt gtatttgatc taggcggtgt
      901 gtccgctgat atttcgttgg acgatcttgc atttggtttt agcaatatgg gtgtactcgg
      961 aatggattat gaggtttttg tttctatacc gccagaaaaa aagctattcc atctgaatag
     1021 tattcttgat caattgctcc cgacgcttct tccgtcactt tcgcctgaat tacaggaatt
     1081 attattagct gcatctggtg ttttaagcat catcgatctg gctccgcaag tgatgaagtt
     1141 aaatgatgtt ctagaggctg ccattgatgc ggtggaagca aaataccctg attcaattga
     1201 atttattgaa acgctcccga tagtcaatgg atattctgaa ggtctgtata aaagaccagg
     1261 cggacacatg tttgcctatg gttttggata tctatatgcc gatataagca atcctttggt
     1321 acctatggca gatattcatt tgacggaaga tggttatgca gcggtagcgg ctttatttga
     1381 agcagaaata gactctgatt ttgggttgtc cattccctaa aaaaacaatc ctaggttata
     1441 tttaaagtat attaaaagtg ctgtatcata gttgattcag cacttttttc tttatcttgt
     1501 ccacttttcc taccggaaat ttgaactttt gttacggaaa aaaatttcac agatatatgg
     1561 ccgctaacaa aacttcagaa attaagcaaa tattaacaga atatttctat gtttaagatg
     1621 gaatgttaac tatgtgctaa cattatttta gacagaggta tttatgcagc ttaaacaaaa
     1681 gaaattgaat ttaaacaaaa tagatgatga catcaagata ttgttgttaa atgaaattaa
     1741 caatcatatt ttactggatg aacagccgtc aatatttctt aactccattg taaacactaa
     1801 aatattcaag gaacatcctt tttatatgct gcacaaacta aaggaaacag aacagtcgcc
     1861 agttcatcat ccggagggaa atgcctggaa tcatacaatg ctggtggttg accaggcagc
     1921 cgggctaaga catattagca gggacgaaaa agcatttatg tgggcggcgc tgctccacga
     1981 tataggaaag gctgaaacaa ccaaaaataa aaggggcaaa ataacagctt ataatcatga
     2041 atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtgaca acaccctacc
     2161 gtccccttgt aaggaaattt gtttgactaa atcatgtaca agctgtatta ttattctata
     2221 gtgtaaaaac gaggtacgtc atgtccaatg tcacagcttt tttgtcttat gttatcataa
     2281 caacatttac accgggacct aataatatta tggcaatgtc caatgcaacg aggtacggtt
     2341 ttaaaaaaag catcaggttt aatgccggag tatttttcgg atttttaatt cttatggtat
     2401 taagcagttt tttgagtgta accttataca gtttaattcc atcaataaag ccggttatga
     2461 cttttatagg ggcttcctac atattatggc ttgcctggaa aatatataaa agcaagcccc
     2521 acgctgaaga cgaaagccat gccaccaata cctttacagc cggaattctt ctgcagtttg
     2581 tcaacgtaaa ggctattatt tattgtatta ccatagtatc aacatttatt gtcccatatt
     2641 atgattctgt ttttgtgctg agtatctttt cgttgtttct ggcttctgtg agttttttat
     2701 ccacatgcag ctgggcggtt ttcgggtttt tatttcagaa attcttattt aaaaatgaaa
     2761 gaattgtgaa tattataatg gcacttttgc tggtttatac tgccctgtcc ttttatctat
     2821 ggggaggggt cgggatgatg ttataatatt atgaatacag cagtaatact tttaataagt
     2881 atgtgtcaag ggatgtgaca acatcacggt acccttgtcg taaaaggagg ttaatatgtg
     2941 ggtaagagca gaacttaaat tcaaagccaa ggaagttttg aagaaaaatt attggaagtc
     3001 gtttttaata agccttgtgc tgttattatc cggaggagac agcagcagca gcggcggttc
     3061 agccggcagc aatagagaga gaatcagtga gtggattgat ttttcggaat atacgataat
     3121 tattgttgca gtaattattc ttgtaattgc tttcagaata ctaataggct atgcattgga
     3181 aacaggctca agaaggtttt ttgtgaaatt agctcagttt aaggatgaca ccggctgtta
     3241 caattttgca tttgataccc tgaattttaa aggtataatc tcaacaatgc ttttgcggga
     3301 tatatatacc tttctttgga ccttgttatt gataattcct ggaattataa aatcttatgc
     3361 atacaggatg actccatata tcctggctga taatcctaac ttgggtgcag acaatgccat
     3421 aactttaagc agaaaaatga tggatggaaa taaatttgaa ttgtttatac tgcaattgtc
     3481 atttttagga tggtacctcc tgggactttt ggcctttgga ataggaatac tgtttgttaa
     3541 tccttattat aatgcaactg aagcacagct ttatctagtg ctcagggaca aggccataga
     3601 tcttggatac tgcaccggtg aggacttaaa tcttaataat gattttatga ggcatgaggg
     3661 ttattattgg gaagaataaa gcataaaagg ccccctttgt gtatatacta acacataggg
     3721 ggtgtttcct ttggatccaa ttgaagtaca ttacaagaac agctttgcgg caattaaaat
     3781 aggagatttt ttggcagaac acatgaataa tgcagcaata gtctgtatcg ggactgataa
     3841 atgcatcatt gacagcttgg ggcccttaac aggtacttta ctgatgaagg aaaatatcaa
     3901 gactgatatc tacggtacct tggaaaaacc tgttcatgca atgaatatca atgaatacat
     3961 aaagaacctt aaaaagaaaa aatatgactg tataatagca gtggatgcct gcttaagcaa
     4021 taagaagaat caaggaatca tagaggttag ggaaggatcc attacaccgg gtaagggcat
     4081 aggaaaattt cttccggaaa tcggggatat atcaataata ggaattgtag attccagtga
     4141 caaggaattt catgatctgg ttcaggacac aaggctttct ctcatttatg aaatggcaga
     4201 aataatctgc ggcggagtat tgaatgcttg cagcatgaat atatctgaaa gctataatca
     4261 ggcttcaatc agcagttttt aaatgtatgg ggacaccagg tgtccccaat tatattgcca
     4321 tctattttaa ggcttccatt gccgcctcaa gctgcaagtc tcttaatgtg ctttcactat
     4381 gttttatcaa tgcgttgtaa agtgcatctt gtgttgtaaa atcaatggtt ccgtatgggt
     4441 acagcagatt atccccctga aacttcttaa gtttctcaaa tgatacctgg tcataaacac
     4501 cgtcaggctt gtttatttca taacctaatt cggcaagtat catttcagcc gctaatacat
     4561 caaggctcac atttcccagc tgaggtttct gctccttgct gaaccttgga tatttggaaa
     4621 tatcaatttt tccgttttcc acaataatat ccggtgtgat tccaatttta tgtacagggg
     4681 ttttgtcaat ggaaaagtat tctgcagttg taaatttcac tccgctgccg tcaatcaagg
     4741 gatacaagga ctgaacaaca cctttgccat aagattttgt gcctacgata attccttcat
     4801 ttttgtactt gactgcacct gcaaatattt ctgttgcact ggcacttccc tcgttaatca
     4861 agacagctat ctcatatttt tgttctttca aggtgctttc ataaacatct tccctgccgg
     4921 ctgcataatc aacatatact actggtccct ctgtaacaaa aaaattcaag agatcaacgg
     4981 cagactgcaa tgttccaccc ccgttgttac gcatgtctat tacaattttt tcaacattat
     5041 tcgcatcgaa cagcttgagt tccttttcta caagctcagc tgtgttctcg gaaaaaccgg
     5101 taaccttcat atatccaata ttatcatcca atattttcac ttcaatattg tttgttacta
     5161 ttgtacttct tatcaaatca aaggcaaggc tttcccttcc gcgaagaatt ccgattttca
     5221 caggtgttcc ttcctctccc cttataagca gggatgcctg gctcgaggtg agtcctgtaa
     5281 tttcataacc gtcaacggtt tttattatgt cgccggcttt gattcctgct tctattgccg
     5341 gcgaaccggg cagaggagta actaccacaa cttgtccgtt tatatccata atttgaacgc
     5401 ctataccgga aaattccccg gcagtatctt caagcaaggc ctggtattcc ttgggagtgt
     5461 agtaagtgct gtagtcgtcc aaaacactga agaatccgtc ataaagacca ttcatgatat
     5521 cctcttcggt cacctggtac tgatacttgt caagtacaaa atatattacc ttcttcataa
     5581 attcaacatc ctggctgaat ttttcttcgt cgatttcaac agcccaagcg ggagtaaatg
     5641 aaagtattac cattaaaact gcaaggaaaa gggagattct tttcttcatg atgcacctct
     5701 aatatttaag attatgtatt tactgtctag catttattga taatgacatc aaggataact
     5761 tcatccgtat tgtccatccc aagatttgaa accctggcta aattttgtat tgagctttcg
     5821 gcactggagc ctaaaatacc gttatcttca ggtatatata tattttccag agccattttt
     5881 gcagcatcga ctgcagcatc gactgatatg gacagtttat aggcacatcc cagctttgcc
     5941 ccgtcacaga gcatacctga aacccctgct atcatattgt ttatggagcc tttaatctgc
     6001 tccttgtcac catccagcat gtatgtcagg cctgctgcac atcccactcc tgctgccact
     6061 ccgcagccgc atacaggtga aagtgctcct agatatactt ttacataaat tgttatcaaa
     6121 tgacttaagg ctacagaccg gaggattttt tcatttggaa cgttttgttc ccgtcctatg
     6181 taggatattg gaattactgc caccagacca tgattgcctg agcctgcact gctcattaca
     6241 ggcagaaggt atcccgacat cctggcctct gaagcagcag cggtatatgc cttagccatg
     6301 gtgaaaacat ctcccgcctt tttatacata taattgccca aaccaacgcc ttgaccttca
     6361 tttaaacctg catccgcaat ttttaaattc atgtcaattc ccttttgaat gaaggaaatc
     6421 tcatctaatg gcaccctatc tgcataatca atcagctcgt cgatagcgaa tttcttaatc
     6481 tcaaatttta tttcattttt tggtttcttt tccaaggtat cagaactatc agctttttca
     6541 aacaagacct gttcattttt tctttcaaaa ataatattgg tatgggaatc ccggataata
     6601 acttctgccg tatccttgtc gccgactgcc tctgctttta tatacagtcc atgagtatta
     6661 ggcttcagct tcaggtcgat aatgtttgaa tccacaattt caattgcctt ctctatactg
     6721 ttatcatcaa catctttaag tacttcaagt aaatattccg atttgcctat aaccagggac
     6781 agggcagccg caaatatgat accgtgctcg tttgtacccg gtatgcctac gctgaatccg
     6841 tttttcagta tgttcttatc aacttcgata ttaagctttt ttacttcaga cccaagtatt
     6901 tctttggcta tggctacccc ataggcaaca gccccgggct cggtgcagcc taatgcagga
     6961 gtaacctgat tttttaatat atctgtaagc agattggttt tcataataca tcccctgtta
     7021 atatatattt attaaattct acactattat agcaaaaata atatatattt acaaataaat
     7081 tatgccacct tgaaataagc ttttgacttt aagctgttta tggggtaaaa tattgtaa
//
LOCUS       DAOPUT010000073         7118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_10532_length_7118_cov_21.200906, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000073 DAOPUT010000000
VERSION     DAOPUT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10532_length_7118_cov_21.200906"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKK75_06525"
     CDS             complement(<1..674)
                     /locus_tag="PKK75_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HNZ83290.1"
                     /translation="MKRFICLLLALMMVLSLAACAQPAQTPPAEEPPAEEQPAEEPPA
                     EEPPAEEEPFEGIIAIVTNTLSQNEEEYRSAELMVKTYGEDKIMHVLWPDNFMTEQEQ
                     MISTIAKIGSNPDVKGLIINQAVPGSNAAVDKLLETRDDIFISYCTPQENPPDVAERA
                     DLILNPDELKMGDAIPVQAQKLGAKTLVHYSFPRHMSIVMLAARRQLMIDKCAEIGLE
                     FYDATAP"
     gene            complement(985..1293)
                     /locus_tag="PKK75_06530"
     CDS             complement(985..1293)
                     /locus_tag="PKK75_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83291.1"
                     /translation="MANIFRSDSEINKIKESKEFKNAASKADDLVNNKQFQDFKNKYQ
                     GKSEEEIMKDAKDMGKKLKQQYGEQEYNKKIEDLKNFEKFLTPEQKRKMRKFLENLDE
                     "
     gene            1507..2217
                     /locus_tag="PKK75_06535"
     CDS             1507..2217
                     /locus_tag="PKK75_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83292.1"
                     /translation="MSKLSGTTRTMAQGIIKDPARMENIIGMVRTFAPLTSTQTVTKL
                     NTYLPAVEKASTLLGMYSFISRAQNFSPIHTVDAKTPADKVAALLKNGNIPVAKLLAK
                     PLISGNMDKIIGAMAMNMAKNGGLNEMLSTLKSGNNNNLNEMLSSLTGGNKDNTDLSS
                     LIETFMPMINNVMSSANKSEESFESSEDDVFKSAQFEHTTTPLSPEKTPRKDIKETKE
                     IKEPYVQKPIRIRQRRRK"
     gene            complement(2251..3204)
                     /locus_tag="PKK75_06540"
     CDS             complement(2251..3204)
                     /locus_tag="PKK75_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNZ83293.1"
                     /translation="MIKTNKTIAELRPGEKADGFYLIKNFEVKKTTSGKQYIDIDLVD
                     KTGEINAKVWDYTKENEKTIAENTIVKVRGEVLEWNGARQLKINKIREKRAEDNVYIQ
                     ELIPSAPIGLDEMLEAVSSYIKKIKDIEIRTLTDNILKKYENKLYYYPAAKRNHHSYR
                     GGLLYHNLRMLQTGEKLCQVYDFLNPDYVYAGVILHDMCKILEMESDEYGIVSDYSME
                     GKLLGHIIQGIKEIEVEGERIGIDREKSVILQHMVLSHHYEPEFGSPKKPMTPEAELL
                     HFLDIMDARMFDFEHSLETIEPGEFTDKIWLLDNRNLYKQK"
     gene            complement(3399..4655)
                     /locus_tag="PKK75_06545"
     CDS             complement(3399..4655)
                     /locus_tag="PKK75_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNZ83294.1"
                     /translation="MKCDEINDRLSLYIDNELSPEEMRQVEEHLQSCEICQKEYEDYK
                     NLISVLNGLPEEEPPEGYCKRLHKKLLENRPQGKAKKQTSKIMSLNRSKWLKYGSIAA
                     ALVLVIAVAGINNFGGMGMKAKNESSYDMSTTEAPAAPQAMPMEPQNGAVAEESMGGY
                     EKDYDYTRDEASHKADDSIMTTSVIDQKEMKIIKTGTISAETEDYDTFLNDLSIKISS
                     VGGFLESNNTEVYQVFNDEKLMYGSIRIRVPQENFYELVDYLENAIEVKRKNINETDV
                     TKDYYEKDNRVKNLEVQEQHLRDLFEKATTVEEMLLIENELRRVRTEIDALNISLSDI
                     DDRAAMSTINLEIQEVMTASLTLKTKDSVWERSRDGFINSVNGIIRSLENLLVGIVSS
                     LPILIPAIIICIVGFLKLRKHWKREI"
     gene            complement(4675..5253)
                     /locus_tag="PKK75_06550"
     CDS             complement(4675..5253)
                     /locus_tag="PKK75_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ83295.1"
                     /translation="MNNHERRLIEESRKGNIDAFEELIKDYKKSAYNIALRVMRNVED
                     AEDASQEALIKIFKNISSFNMDSTFKVWMYRIVVNTCLDFKRKKSLNAISLDETLDLG
                     SGREVQREISDETSNPDALVDRNYSTGLVDDAINMLEDDFKTIIILRDIKGFTYDEIS
                     EILNCNLGTVKSRLSRARKRLKVLLEKEMKAG"
     gene            complement(5424..6929)
                     /gene="hutH"
                     /locus_tag="PKK75_06555"
     CDS             complement(5424..6929)
                     /gene="hutH"
                     /locus_tag="PKK75_06555"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNZ83296.1"
                     /translation="MDKVIITGNSLTLEEVNAVCRNYEKVELSNLAVEKILESRKVVD
                     DFVDNEDVVYGITTGFGKFSDVTISKEESKTLQKNLIITHAVGAGKPFDTEIVRGIML
                     LRINNLAKGYSGARLETIQTMIVMLNKKVHPVVPEKGSLGASGDLAPLSHMVLPMIGL
                     GQAEYKGEILTGQEAMEKSGIPVIQLTAKEGLALINGTQVMSAVGALTVYDSLNILKV
                     ADIAAALSFEAQNGIIDALDKRLHDVRPHKGQIDTARIMKELLTGSKMTSRQGQIRVQ
                     DAYSLRCVPQVHGASKDAINYVKEKVEIEINSVTDNPIIFPETKEGISGGNFHGQPMA
                     LCFDFLGIALSELADISERRIERLVNPTLSGLPAFLVEHGGLNSGFMIIQYSAAALVS
                     ENKILAHPASVDSIPSSANQEDHVSMGTIAARKAREIMENVRRVLAMEIMCACQGIDL
                     RGNKGLGAGTKPVYEKVRKAVPMLKEDRPLYEDINKCEELIIKGTITEIGE"
BASE COUNT         1862 a         1560 c         1149 g         2547 t
ORIGIN      
        1 ggtgctgtag catcatagaa ttcaagacct atttctgcac atttgtcaat cattaactga
       61 cgtcttgcag caagcattac tattgacata tgtcttggga atgagtaatg taccagtgtt
      121 tttgctccca atttctgtgc ttgtacagga atagcatctc ccatcttcaa ttcatcggga
      181 ttaaggatca ggtctgctct ttctgcaacg tcaggaggat tttcctgtgg tgtacaataa
      241 gaaataaata tgtcatcacg agtttccaaa agtttatcta cagctgcatt tgatcccgga
      301 acagcctgat tgataatcag tccctttaca tcaggatttg atcctatctt cgcaattgta
      361 gaaatcattt gctcctgttc agtcatgaag ttatcaggcc ataatacgtg catgatttta
      421 tcttcgccat atgtttttac cataagctca gctgaacggt attcttcttc attctgagaa
      481 agagtgtttg ttacaatggc tattatgcct tcaaacggtt cttcttctgc cggcggttcc
      541 tctgccggtg gttcttctgc cggctgctcc tctgcaggcg gttcttctgc cggtggtgtc
      601 tgtgccggct gtgcacatgc tgctaacgat aaaaccatca ttagagccaa caataaacat
      661 atgaatcttt tcattacttg tccctcctaa tttacacaaa ttaaaaatat gtatccctat
      721 tataaatgaa tataattctt atgtcaaact atttttgaaa acgccatcca cgtttgctaa
      781 acaatttctt aattttgcac ctgtaaagta tgcgttttga tttgttgttt taatctttaa
      841 actgttggaa aatagcaatt gttcgcagat cttatacttt gtatatgttt aatttttcta
      901 taataaaaga ggcttacgct ctctttggcc atgtatgaaa tgccatcttt gtcactagac
      961 caagaagaag ggagcttttg ctccctattc atcaagattt tccaagaact ttctcatttt
     1021 tcttttctgt tccggtgtca ggaatttctc aaaatttttt aaatcttcta tttttttatt
     1081 gtattcctgt tccccgtact gctgttttag ttttttgccc atatcttttg catccttcat
     1141 aatctcttct tctgattttc cctgatactt atttttaaag tcttgaaatt gcttattatt
     1201 tacaagatca tctgccttgg atgcagcatt cttaaactct ttgctttcct tgattttgtt
     1261 tatttcgctg tcgcttctaa atatatttgc catattctac ctcctatatg ttatatgata
     1321 tgccgctgtc accgtttaat tacaattttc tgcttaagct cttgctgcat actatgtaac
     1381 ataattgaat catcggaata tgatatacgg agatatttat aagaaggtga taatattcca
     1441 gttcaaatat tattatatgt tttagcagca aattatattt ttggaaacaa tcagtttaac
     1501 ggtgttgtgt ctaaactttc aggaacaacc aggaccatgg ctcaaggaat aatcaaggac
     1561 ccggccagaa tggaaaatat tataggcatg gtccgcacct ttgcccctct tacatcaacg
     1621 caaacagtaa caaagttaaa tacatatctg ccggcagtgg aaaaagcaag cacattgctc
     1681 ggcatgtatt catttatcag cagggcacag aatttcagtc ctattcatac tgttgatgcc
     1741 aagacaccgg cagataaagt agcagcgctg ttaaaaaacg gcaatatccc tgttgcaaag
     1801 ctattggcaa agccgctcat atccggaaat atggataaga taattggtgc catggcaatg
     1861 aatatggcta aaaacggcgg tctaaacgag atgctttcca cattaaaaag cggcaataac
     1921 aacaacttaa atgaaatgct ttcttcattg acaggcggaa ataaggataa tacggattta
     1981 agcagtttaa tagaaacatt tatgcctatg ataaacaatg tcatgtcctc tgcaaataaa
     2041 tctgaggagt cgtttgaatc ttccgaagac gacgtattta agagtgctca gtttgagcac
     2101 accaccacgc ccctttcacc tgaaaaaacc ccgagaaaag atataaaaga aacaaaagaa
     2161 ataaaggaac cctatgtaca aaagcctatt cgcatcagac aaaggagaag aaagtaagga
     2221 atgtccccgt aagttcgggg acattcttaa ttatttttgt ttatacaggt ttctgttatc
     2281 caaaagccat attttatcag tgaattctcc cggctcgata gtttcaagag aatgttcaaa
     2341 atcaaacatc cttgcatcca ttatatccag gaagtgcagg agctcagctt ccggtgtcat
     2401 cggcttcttg ggactgccaa actccggctc atagtgatgg gataagacca tgtgctgcaa
     2461 aattacgctt ttttccctgt ctatgcctat cctttcacct tcaacttcaa tttcttttat
     2521 gccttgaatt atatgcccga gtaatttgcc ttccattgaa taatcagaaa ctattccgta
     2581 ttcatcagat tccatttcca atattttgca catgtcatgg agaattactc ctgcatatac
     2641 gtagtccgga ttaagaaaat cataaacctg gcatagcttc tcaccggtct gaagcatcct
     2701 caggttatgg tacaaaagac ctcccctgta tgaatggtgg tttcttttag cagcagggta
     2761 ataatacaat ttattttcat attttttaag tatgttatct gttaatgttc tgatttctat
     2821 atcctttatt ttttttatat aggagcttac cgcctcaagc atttcatcaa gaccgattgg
     2881 cgcagaaggt attaattcct gaatatacac attgtcttca gccctttttt cccgtatttt
     2941 attgatctta agctgtctgg ctccgttcca ttccagaact tctcctctta cctttacaat
     3001 tgtattttct gcgattgttt tctcgttttc ctttgtgtaa tcccatacct ttgcatttat
     3061 ttcgccggtt ttgtcaacca ggtcaatatc aatatactgt tttccgctgg tagttttctt
     3121 tacttcaaaa tttttaatca gataaaatcc gtctgctttt tctcccggcc ttaactcggc
     3181 gattgtttta tttgttttaa tcatgctgtt catctgttct cttgctttat catccagcat
     3241 atctgaaata ctctgaccca tagtctgccc ctctcataat ttgttgcttt attgattgta
     3301 ttcattatac catacttatc cgaatcaata aacagtattt tacaaaagaa aaacacaccc
     3361 gtgggatagg tgtgttcaat tcggcttaaa aagcggggtt aaatttcccg tttccaatgc
     3421 tttcttaatt taaggaaacc aactatacat atgattattg caggtatcaa tattggcaaa
     3481 cttgatacta ttccaacaag cagattttcc aggcttctta ttattccgtt aacggaattt
     3541 ataaaaccat cccttgatct ttcccatacg ctgtccttag ttttcaaggt aaggcttgca
     3601 gtcatcactt cctggatttc caggttaatt gttgacattg ctgctctgtc gtctatatcg
     3661 ctcaagctga tattcaaggc atcaatctcg gttcttactc ttcttagttc attttctata
     3721 aggagcattt cttcaactgt tgttgctttt tcaaaaaggt ctcttaagtg ctgttcctga
     3781 acctccaggt tttttaccct gttgtccttc tcataataat ccttggttac atctgtttca
     3841 ttgatattct ttctttttac ttcaatggca ttttccagat aatcaaccag ttcataaaag
     3901 ttttcctgag gaactctgat tcttatgctt ccatacatta atttttcatc attaaagacc
     3961 tggtaaactt cagtgttgtt gctttccagg aatccgccca cagagcttat ttttattgac
     4021 aagtcattta aaaatgtatc ataatcctct gtttcagcag aaatcgttcc ggtcttgata
     4081 attttcattt ctttttggtc tattacgctt gtcgtcatta tagaatcgtc tgccttatga
     4141 gaagcttcgt ctcttgtata gtcatagtct ttttcataac ctcccataga ttcttcagct
     4201 actgctccat tttgtggttc catgggcatt gcttgagggg ctgcaggtgc ttccgtcgta
     4261 gacatatcat atgaagattc gtttttagcc ttcatcccca tgccgccaaa attgtttatg
     4321 ccggccactg caattaccag caccaatgca gcggcaatgc ttccgtattt cagccattta
     4381 ctcctgttga ggctcattat ttttgaagtt tgctttttag cttttccttg aggcctgttc
     4441 tccaataatt ttttatgaag tcttttgcag taaccttcag gcggctcttc ctccggcaaa
     4501 ccattcaata ctgatattaa atttttataa tcttcatact ctttttgaca aatttcgcaa
     4561 gactgcaggt gttcttccac ttgccgcatt tcttcggggc ttaattcatt atcaatatat
     4621 aatgacagtc tgtcatttat ttcatcacat ttcatattat caccacctta gctgttagcc
     4681 agccttcatt tccttttcca atagtacttt taatcttttc cttgctcttg acaatcttga
     4741 tttcacagtc cctaaattgc agtttagtat ttctgatatt tcatcatagg tgaatccttt
     4801 tatatctctt agtattataa ttgttttaaa atcatcctcc agcatattga ttgcatcatc
     4861 tacaagccca gtgctgtaat tcctgtcgac caaggcatcc ggattgctgg tttcgtcaga
     4921 aatttctctt tgaacttccc tgcctgaacc tagatctaac gtctcatcta aagaaattgc
     4981 atttaaactt tttttacgtt taaaatccag gcaagtgttt actactatcc tgtacatcca
     5041 tactttaaat gtggagtcca tattaaaact gcttatattt ttaaatattt ttattagtgc
     5101 ttcctgtgaa gcatcttctg cgtcttcaac atttctcatt acacgaagag ctatgttgta
     5161 ggcacttttt ttgtagtcct ttataagctc ttcaaaagca tcaatattgc ctttccgact
     5221 ttcctctata agccgccttt catggttgtt catctttgtt ccaccccgtt cggttatata
     5281 ttagacgtta cattttataa attgttccac aaatttttta ttttatttaa ttataacact
     5341 ccggcttgta ttattcaata aaaaatagca gggctgcccc tgccataaat tcgctgcgta
     5401 aataaccatg gaatcctgcg gtctcattcg ccgatttctg taattgtccc ctttataatt
     5461 aattcttcac atttattgat atcctcataa agaggtctgt cttctttcag catggggaca
     5521 gcttttctta ctttttcata aaccggcttt gttcctgccc ccagcccctt atttcctcta
     5581 aggtctattc cctggcatgc gcacattatt tccatagcca gaacccttcg tacattttcc
     5641 ataatttctc ttgcctttct tgcagcgata gtccccattg aaacatggtc ttcctgattt
     5701 gcagaggatg gtatgctgtc aacagaggca ggatgggcta atattttatt ttctgaaacc
     5761 aatgctgctg cagaatactg aataatcata aatcctgagt tcaatccccc atgttccacc
     5821 aaaaatgcag gcagtccgct gagcgttgga tttacaagcc tttcaattct tctttcggaa
     5881 atatcggcaa gttctgaaag ggcaatcccc aaaaagtcaa aacaaagtgc cataggctgg
     5941 ccgtggaagt ttcctcctga aataccttcc tttgtttccg gaaatattat gggattgtct
     6001 gtaaccgaat ttatttctat ttccactttt tcctttacat aatttattgc atccttgctt
     6061 gctccgtgga cctggggaac acatctcaga gaataggcat cctggactct gatttgtccc
     6121 tgtctgcttg tcattttgct gccggtcaaa agctccttca ttattctcgc agtatcaatc
     6181 tgccccttgt gaggacgaac atcatggagt cttttatcca gtgcatcaat tattccgttt
     6241 tgagcttcaa agcttagggc tgctgcaata tccgccactt ttaaaatatt aagggaatca
     6301 tagacagtca gagccccaac ggcagacata acctgtgtcc cgttaataag ggcaagccct
     6361 tccttggctg taagctgtat tacaggtatt cccgactttt ccatagcttc ttggcctgtt
     6421 agtatttctc ccttgtattc tgcctgtcca agtccaatca taggcagtac catgtgggat
     6481 aagggtgcta aatctccgct tgcacccagt gagccttttt ccggtacgac aggatgaact
     6541 tttttgttaa gcatcactat cattgtttgt attgtttcaa gcctggcgcc cgaataacct
     6601 tttgccagat tatttattct aagcagcatt attcctctta caatttctgt atcaaatggt
     6661 ttccccgcac ctaccgcatg agttattatc aagtttttct ggagcgtttt tgattcttcc
     6721 tttgatattg ttacatcgct gaattttccg aagcctgttg taatgccgta taccacatct
     6781 tcattgtcca caaagtcatc aacaaccttc cttgactcca aaatcttttc aactgcaaga
     6841 tttgacaatt ccaccttttc ataatttctg caaacagcat ttacttcttc taatgtcaaa
     6901 gaatttcccg ttattataac cttatccatt gctgcctccc attaaaaata aaggtaaatt
     6961 ccagcttcta aggtgccaaa acttacctct taatatcaaa ttttctttag gctgatttcg
     7021 tctatattgt aaactatcat atttattttg tcaatcagcg ttctttaaaa tagcctgcgt
     7081 ttcattcttt aaaacagcct gcgcctcaac aactgcag
//
LOCUS       DAOPUT010000074         7075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_10606_length_7075_cov_16.820085, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000074 DAOPUT010000000
VERSION     DAOPUT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10606_length_7075_cov_16.820085"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1149
                     /locus_tag="PKK75_06560"
     CDS             19..1149
                     /locus_tag="PKK75_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ83297.1"
                     /translation="MDRKFIEKFGLSLPRLGYGCMRFPVKDDKIDFEYARDLIHHAYE
                     KGINYFDTAYNYHGGESETFLGKVLTEYKRDSYFLTSKLPVWKVKEEGDAEKLFNEQL
                     EKCNTEYFDFYLLHSLHKESIETVEKFNLYDFILKKKKEGKIQNIGFSFHDSNEVLQK
                     FASAHQWDFIQLQINYWDWEENDAKILYETLENLNIPCFVMEPVRGGFLASFAPHAMK
                     RFKDFHPDKSIASWALRWVASLPNVALILSGMSTMDQLEDNIETFTGIKPITDGEQKV
                     IDQVVEDLRKINPIPCTGCRYCMDCQYGVDIPGVFGIYNDYKKSENKDIAKRNYFTFM
                     AEKEQGGNCQKCLNCIQNCPQHIDIPVELEKIHKEMLSIKSE"
     gene            1417..>2720
                     /locus_tag="PKK75_06565"
     CDS             1417..>2720
                     /locus_tag="PKK75_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNZ83298.1"
                     /translation="MKKEKIIIGTLMLTVATTSVRFVGMFFRIYLANTIGSEGIGLYQ
                     LILSFFFLMVTLATSGIRVAISKLVSEEVALGNYTNAKNVLRQSIGISIITGFAAGAV
                     LYFFADYIGSHILNDDRTVLALLYLAPSLPVMAVSNCYKGYFYALGKVTQPSIIMMTE
                     QFVRIFLIIYLMGTFLPKGLGYACAAIAIGMTAEEIFALFFVWFFYIFDKKPGLNKPR
                     KKSDNMIFKILRISVPLSGTSYINSILRTVENTLIPARLLLYGLSSETAISLYGMIKG
                     MVLPLLFFPSSILTSLSSLLIPAVAGDNALANEKSVSRTLSQVIHFTAISGIWVVAVF
                     VSFPDEIAMAVYNDHQVGIMIKLISYVCPFMYLNMVISSMLNALGEQVSSFVVNIVES
                     VLKISIIYLFVPVYGFNAYLFALFITTILNTILYLFRLLQVS"
     assembly_gap    2721..2820
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2907..3458
                     /locus_tag="PKK75_06570"
     CDS             2907..3458
                     /locus_tag="PKK75_06570"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HNZ83299.1"
                     /translation="MQLDIMNFLSFFLVYGALGFLLETVFRSLATGKVYISRGFITNG
                     FCPLYGISGIIIIEIFTVCELSIVNPLHALIGATAGSMAVVTLFEYITGRMLDRAFHF
                     KLWDYSGNTFNLHSYICLEFTLLWGILALLLVYFIHPVIEVLVYAIPYNIRTLAVVMF
                     LSMIYVNASYNVRKMHKLNVRKL"
     gene            complement(3461..5080)
                     /locus_tag="PKK75_06575"
     CDS             complement(3461..5080)
                     /locus_tag="PKK75_06575"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HNZ83300.1"
                     /translation="MKYNLIFGHKTPDTDSVCSAITLTNLKNKLNEPSKAYILGNINK
                     EAAFVLKYFNIDVPEILGNVKIQIKDLDFERVKPFSKHKSVHFAYFHMNENKLRTLPI
                     VDEENNLCGLITMKDIAMSLINTDQHYLCTSYDNIIETLLGNSILKFDEIIKGNVIVA
                     AFEEKTLRKSDIVNENSIVITGDRYDVIEYAIESKARLIIVTGDLKVTDELIEMAAEN
                     KVNIIGTGYQTYYTAKNISLAKYVDDIMKKNDIMLFSEDDYLNDCKEDIEQSKHSKFP
                     IVSKDKKYLGLLSRSHLINPKRKNVILVDHNEMNQSADGIEEAEILEVIDHHKIGDIK
                     TSIPISFRNTPVGSTSTIIFQMYRENNVEITKDMAGLMLSGIISDTLLFKSPTTTERD
                     KYAVNELLRIVDIDLYQYAMDMFRAGTSLEGKSLEEIIYQDFKKFNLVYKNVGISQVF
                     TLDIKEIMNRKQEFVKLIDDITHENYYLIIMAVTDIVNEGSYIFYTSSREKLVRMIFQ
                     EDVHQGIYIDKCVSRKKQIVPRVINALNQLK"
     gene            complement(5077..6066)
                     /locus_tag="PKK75_06580"
     CDS             complement(5077..6066)
                     /locus_tag="PKK75_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ83301.1"
                     /translation="MRTINKQIIENIMTGYNKKRLRASHEADLKREKLYKEIPRLAEI
                     DKEMRLFSIKLSKSFLLQPEDLDRQVVKLRQEIEVLKKERQLIYENYNISRNYLDIEY
                     ECQKCGDTGYTPDGKRCSCFNRQIIDSLFQASNMINMLGKENFNTFDINIFSNDSYRN
                     EKLTPRQNMYYILEISEDFCSNFHDAGVNLLFYGGTGLGKTFMCNCIANELLNKEISV
                     LYQTSFSLFETVENHKFNKLNESEENLMNYNMIFDCDLLIIDDLGTEVSNSFTNAELF
                     NIINERMITEKRTIISTNLSLEQLRDTYSDRITSRIFNNFVPLKFYGKDLRFK"
     gene            complement(6038..>7075)
                     /locus_tag="PKK75_06585"
     CDS             complement(6038..>7075)
                     /locus_tag="PKK75_06585"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HNZ83302.1"
                     /translation="DTPIDNIFINDYMPQAEGTYVKVYLMGYKCARDRQDFNNETLAK
                     NLKLPLADVLNAWNYWESEGIVIKHETDDEYNYAVEFVNLKQYYMDKVYKHIRNVDDK
                     NKYVNNEQLISTAKNPEIKTMMEEIEDMLGRPLKISEKQKIITWLNKYKTKPDLMTQA
                     FSYCVNNKKVKRFSYIESVVSAWYDAGVVDLDTMVDYLEKRNDRFSDYSRISKALGFN
                     RTLTEAEMRTIDRWIDEWDFSMEMVLRCLDNSTKINNPNLNYFDKILSEWHKNGFKTI
                     EDLKSDIKPEKTKKEGSKAKNKFHNFIQSEDISGDDLEKIARERFKKKLNKLGLEIPE
                     EGDEDNKQTNN"
BASE COUNT         1999 a         1338 c         1252 g         2386 t
ORIGIN      
        1 acttaaagga aggttgttat ggatagaaaa tttattgaaa aattcggttt aagcttacca
       61 agattaggct acggctgtat gcggtttccg gtaaaggatg ataaaattga ttttgaatat
      121 gccagagacc tgattcatca cgcttacgaa aagggtataa attattttga cactgcttac
      181 aactatcacg gtggagaatc agaaacattt ttaggaaaag tattgactga atataagaga
      241 gacagttatt ttttaacaag caagcttccg gtatggaagg taaaagaaga gggagatgca
      301 gaaaaactct tcaatgaaca gcttgaaaaa tgcaacacag aatattttga cttttatctt
      361 cttcattcct tgcataaaga atccattgaa acagttgaaa agtttaatct ctatgatttt
      421 atacttaaaa agaaaaagga aggaaaaatt caaaatatag gtttttcatt tcatgacagt
      481 aatgaagtgc tgcaaaaatt tgcttctgcc catcaatggg atttcattca gctgcagata
      541 aattattggg actgggaaga aaatgatgca aaaatcttat acgaaaccct ggaaaattta
      601 aacatcccct gctttgtcat ggaaccggtg agaggaggtt tcctggcttc ctttgctccc
      661 catgcaatga agcgcttcaa ggacttccat ccggacaaaa gcattgcatc ctgggccctt
      721 agatgggttg catcccttcc caatgttgct ttgattttaa gcggcatgtc aaccatggac
      781 cagcttgagg ataatataga aacatttacc ggtataaaac caataaccga tggagagcag
      841 aaggttattg accaggtggt tgaagacttg agaaaaatca accccattcc ctgcacaggc
      901 tgccgctatt gcatggactg tcagtatgga gtggatattc caggtgtttt tggcatatac
      961 aatgactaca agaaatcaga aaacaaagat attgcaaaac gcaattattt taccttcatg
     1021 gctgaaaaag agcagggagg aaattgtcaa aaatgcttga attgtattca aaactgtccc
     1081 cagcatattg atatcccggt tgaactggag aaaattcaca aggaaatgct gtcgataaaa
     1141 tccgaataag atgaatagcg gcagatcatt cgcaaggagc tcaaattttt agagacaatt
     1201 ataggtactg gaacgcattc cgctataggg attgtctttg aggttagagg aacgtccccg
     1261 aattgaaaaa ttgtgcagcg ggactgcgag ctcttatctt tttaaatatg tcagatataa
     1321 atttaatgca atggttctcc attgcatttt tttatgtcta aaataaattc aaatattttg
     1381 catatagtta tattagtaaa atttacgagg tggggcatga aaaaggaaaa aattattatc
     1441 ggtacattga tgttaaccgt ggctactaca tctgtccgct ttgtgggcat gtttttcaga
     1501 atttacttgg caaataccat aggatcggaa ggaataggcc tgtatcaatt gattctttcc
     1561 tttttctttc tcatggtaac ccttgcaacc tcaggtataa gggttgctat ttcaaaactg
     1621 gtttcagaag aagttgcctt gggcaattat acgaatgcta aaaatgtcct tcgccaatcc
     1681 attggtatat ccataatcac aggttttgct gcaggagctg tgctttattt tttcgctgat
     1741 tatataggct ctcatatatt aaacgatgac agaacagtat tggcattgct gtacctggct
     1801 ccaagccttc ctgtaatggc agtttcaaac tgttacaaag gttattttta tgccctgggc
     1861 aaggtcactc agccctcgat tataatgatg actgagcaat ttgtaagaat atttctgata
     1921 atttatctca tggggacatt tcttccaaaa ggactggggt acgcttgtgc tgcaattgcc
     1981 ataggaatga ctgcagagga aattttcgct ttgttttttg tatggttttt ctatatattc
     2041 gataaaaagc ccggtttaaa taagccccgg aaaaaatcag ataacatgat ttttaaaatt
     2101 ctccggattt cagtacccct gtcagggaca tcatatataa actcaatact gagaaccgtt
     2161 gaaaatacat tgattcctgc aagacttcta ttgtacggcc tatcctccga aacagccata
     2221 agcctctatg gaatgattaa aggtatggtg cttcccttat tgttctttcc ttcttctata
     2281 ctcacatccc tttcatccct cttgattcct gccgtggcag gtgataatgc gcttgccaat
     2341 gaaaagagcg tttcaagaac cctgtcccaa gtaatacatt ttactgccat ttcaggaata
     2401 tgggtggtgg ctgtatttgt atcattcccg gatgaaatag ccatggctgt ctacaatgac
     2461 caccaggtag gcataatgat aaaattaata tcatatgtgt gccccttcat gtatttgaac
     2521 atggttatat cgtccatgct caatgctctg ggggagcagg taagctcttt tgtagtcaat
     2581 atagttgaat cagtgttaaa aatctctatc atatacttat ttgttcctgt ttacggattc
     2641 aatgcatatt tgtttgcact gtttataact acaatactca atacaatctt atacctgttc
     2701 agactgctgc aggtatcaag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 aaaaaacaaa aaaagcataa attgccatat atatcaaagc atatttctta aattactcca
     2881 aagaatacaa tttgaggagt gagaatatgc aactggatat tatgaatttt ttgtcgtttt
     2941 ttttagtata cggagccctg ggtttcttat tggaaacagt ttttagaagc cttgcaaccg
     3001 gtaaggttta tataagcagg ggattcatca caaatggatt ctgtccttta tacggaatca
     3061 gcgggattat aataatcgaa atttttacag tgtgtgaact ttcaatagtc aatccattac
     3121 atgctcttat tggcgcaaca gccggcagca tggctgtagt aaccctgttt gaatacatta
     3181 cgggaagaat gcttgacagg gcatttcatt tcaaattatg ggattacagt ggaaatacct
     3241 ttaatctgca ttcctatata tgccttgaat ttacattgct ttggggaatt ttagctctgc
     3301 tgctggtata ctttattcat cctgtaattg aggtactggt ctatgccata ccatataata
     3361 tcagaaccct tgccgtagta atgtttttat caatgattta tgtaaatgca tcctacaatg
     3421 ttcgaaaaat gcataagtta aatgttagga aattataaaa ttatttcagc tggttcaggg
     3481 cgttaatcac ccttggaact atttgttttt ttcttgaaac gcatttatcg atgtagatac
     3541 cttgatgaac atcttcctgg aaaatcatcc tcaccagctt ttcccttgat gaggtgtaga
     3601 aaatataaga accctcgtta acaatgtcgg ttacagccat aattatcaaa taatagttct
     3661 catgggttat atcatcaata agctttacaa actcctgttt tctgttcatt atttccttaa
     3721 tgtcaagggt gaatacctgt gatataccca cattcttata aaccaggttg aattttttga
     3781 aatcctgata gatgatttct tccagggact tgccctccag ggatgttcct gccctgaaca
     3841 tatccattgc atattggtat aaatcaatgt caacaatcct taaaagctca ttaactgcat
     3901 atttgtccct ttcagttgtg gtaggcgact taaaaagcag ggtgtctgaa attatgcccg
     3961 acagcatgag tccagccata tcttttgtta tttccacatt attttccctg tacatttgaa
     4021 aaataatggt gcttgtgctt cccacaggtg tgtttctgaa gctgattgga attgaagtct
     4081 tgatatcccc gattttgtgg tggtctataa cctccagtat ctccgcctcc tcaatgccgt
     4141 cggcactctg attcatttca ttgtggtcca ccagtatcac attttttctc ttgggattga
     4201 ttaaatggct tctgctaagt agtcccagat attttttatc ttttgatact atgggaaatt
     4261 tagaatgctt tgactgctct atatcctcct tgcagtcatt taaataatcg tcctcggaaa
     4321 atagcatgat atcatttttc ttcatgatat catccacata tttggcaaga gagatattct
     4381 ttgccgtata atatgtttga taaccggtgc ctataatatt aaccttgttt tcagcagcca
     4441 tttcaatcag ctcatcagta accttcaaat cccccgttac aattatgagc cttgcctttg
     4501 actcaatggc atattctatg acgtcatatc tgtctcctgt gattacaatt gaattttcat
     4561 ttacaatatc agattttcgc agggtttttt cctcaaaggc ggcaactatt acattcccct
     4621 tgattatttc atcaaacttc aaaatagaat ttccaagcag cgtttcaata atattgtcat
     4681 aggatgtgca caaataatgc tggtctgtat tgataagact catggcaata tccttcattg
     4741 taatcaagcc gcataaatta ttctcttcat caactatggg aagggttcta agcttgtttt
     4801 cattcatgtg aaaataagca aagtgcaccg acttgtgctt gctgaagggc tttacccttt
     4861 caaaatccag gtcttttatc tgtattttta cattgcccag tatttcaggc acatcaatat
     4921 tgaaatattt taatacaaaa gcagcttctt tgttgatatt tcctaaaatg tatgcctttg
     4981 atggctcatt aagcttgttt tttaaattgg tcagagtgat tgccgagcag acgctgtcag
     5041 tatccggagt tttatgacca aaaattaaat tgtatttcat ttgaatcgga ggtcctttcc
     5101 gtaaaattta agggggacaa aattattgaa tatccgggat gtaatcctgt ccgaataagt
     5161 gtccctgagc tgctccaagg acaagttggt tgaaattatg gttcttttct cagtaatcat
     5221 tctttcgttg ataatattga aaagctcagc attggtaaat gaattgctta cctctgttcc
     5281 taggtcatct ataatcagca aatcgcagtc aaaaatcata ttgtaattca tcaggttttc
     5341 ttcactttca ttaagcttgt tgaacttgtg gttttccact gtttcaaaca aggaaaatga
     5401 agtctggtac aaaacagaaa tttctttgtt caaaagttca ttggcaatgc agttgcacat
     5461 aaatgtctta cccaggcccg ttcctccgta aaacaagagg ttcacaccgg catcatgaaa
     5521 gttgctgcag aaatcctcgc ttatttcaag gatatagtac atattctgcc tcggtgtaag
     5581 cttttcattc ctatagctgt cattgctgaa aatatttata tcaaatgtat taaaattttc
     5641 tttccccagc atattaatca tattggaggc ttggaaaagg ctgtctatta tctgcctgtt
     5701 aaaacagctg caccttttgc cgtcaggtgt ataaccggta tcgccgcact tttggcattc
     5761 atattcaata tcaagataat tccgggatat attgtaattt tcgtatatta actgcctttc
     5821 cttttttagg acttcaattt cctgccttaa ttttacaacc tgcctgtcca aatcttcagg
     5881 ctgtaataaa aagctctttg acagcttgat gctgaataat ctcatctcct tgtcaatttc
     5941 agccaggcgg ggaatttcct tataaagctt ttcccttttc aggtctgcct catgtgaagc
     6001 cctcaatctt tttttattgt aaccggtcat tatattttca attatttgtt tgtttattgt
     6061 cctcatctcc ttcctccgga atttccagac ccagcttgtt cagcttcttt ttaaaccttt
     6121 cccttgcgat tttctctaaa tcatcccctg aaatatcctc gctttgaata aaattatgaa
     6181 atttattttt tgctttggag ccttctttct ttgttttttc aggttttatg tcgcttttta
     6241 agtcctcaat agttttaaag ccgtttttgt gccattcgct gaggattttg tcgaagtaat
     6301 ttaagttggg gttgttgatt tttgtagaat tatcaagaca ccttaaaacc atctccatgg
     6361 aaaaatccca ttcatcaatc catctgtcga ttgttctcat ttcggcttca gtgagggttc
     6421 tgttgaatcc cagagccttg cttattcttg aataatcaga aaacctgtca tttcgttttt
     6481 ccagatagtc taccattgtg tccaaatcca ccacccctgc atcataccaa gcggaaacaa
     6541 cagattcaat gtaagaaaac ctctttacct ttttgttgtt gacgcagtag ctgaaagcct
     6601 gagtcatcaa atcgggtttc gttttatatt tgttgagcca ggttataatt ttttgctttt
     6661 cacttatttt taaaggcctc cccagcatgt cctcgatttc ttccatcatt gtttttattt
     6721 cagggttttt agcagttgat atcagctgtt cattattcac atacttgttt ttatcatcaa
     6781 cattccgaat atgtttgtac accttatcca tataatattg ctttagattt acaaattcaa
     6841 ctgcataatt gtactcgtca tccgtttcat gcttgattac tataccttcg ctttcccaat
     6901 aattccaggc attcaataca tctgccaggg gaagcttcaa attcttggca agggtttcat
     6961 tgttgaagtc ctgcctgtcc ctggcacact tgtatcccat caggtatact tttacatagg
     7021 ttccctcagc ctgaggcata tagtcattta taaaaatatt gtctatagga gtatc
//
LOCUS       DAOPUT010000075         7013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_10717_length_7013_cov_19.882006, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000075 DAOPUT010000000
VERSION     DAOPUT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10717_length_7013_cov_19.882006"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="PKK75_06590"
     CDS             complement(<1..1598)
                     /locus_tag="PKK75_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ83303.1"
                     /translation="MRKFKKLFLLIVTFVLLSTRPVLARTVFYDTFNHWAESDINFAS
                     NNMKVFVGYGDFTFRPENNITRAEFIAILSRTAYRQNQMKEIYTGDLSYSDMSDKHWS
                     YTFIISMNEYLKTNKFTLNDIFPGSNFYPDRAITREESAALIAAFSKGALFDNPLTFT
                     DLPADNKYYSEIQRLVNAGIIVGYENNTFRGSTNITRAESASLIRRVYTDIKTSTGGN
                     YLTKLEFMPIKGEEMYSYFGNYDLNTTNTNDRKFIKAKDTLEYLAFGGYIFPEDSHLY
                     DMDAVKTMESLRTSGYFNIVGTNYYMIMFGSYPNAEKSKLANEILTNVIARNDLKDAE
                     MMQIFTLISKYDVSETLYMGALEKWNKLTGSDNSKANIIFFRYAYYIKNDNKDMLRVL
                     IYEDLKKDNKIPSILNVDFRPNPDTSGVDFRDYSFGSYSFSLYKDTTLLRYLRNLTMP
                     LSQNSKVVELPNLLMIEKNPKPAVTMGGYENMFTKYSINKLYVLYFLGEKERTFVEAM
                     NDYDIIKTFEMYKKSPTGFDDTYTG"
     gene            complement(1617..3425)
                     /locus_tag="PKK75_06595"
     CDS             complement(1617..3425)
                     /locus_tag="PKK75_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNZ83304.1"
                     /translation="MDKSLIREIKCIINEIEEHDYISKVKYVIFLICKELYNSKNEYP
                     VKNPSIFKEYSNFDLKDKKAEKRIEEFTGINEGNIKVSSFYPASLYESLLTGKEKKSL
                     GQVYTPFEIIDKMLCEVFAIKKIDGSVRILDPSCGGGYFLIELYKYISSTHPELDKRY
                     IAENMLFGIDIDDFSIFLSKIGLIFHTGLDNIKFNIYKGDFLIDNINMDKFDIIIGNP
                     PYVGHKNSSASYKRLLYEKYSGVFYDKADVFYCFFNKGKEELKTDGIIAFITSRYFME
                     ALYAEGLRNFIKENFNIVKIIDYKGEPAFKRAMVSPAIIILSNFWNKSIFSYVKKDKD
                     VFESYEYAQDKLKDSGWIILKDEDEKLFEKIDSISNTYIKDVCTIKQGIITGCDKAFI
                     LTEEVIEKYGIERFLLRKWIKNSNISKKNIKYNNLYLLYSDMIENENDCSNAINYLSG
                     YKDKLMNRRECKSGLRKWYELQWGRSKSDYDNPKIIFPYKSKQNNFYYDDEAYYCSAD
                     IYLMNNFSKDVPVDYLISYLNSEIFEFYLKCQVKRVGKNVYEYYPNKINNLKIYLPQK
                     NTAQNFSDLENFSIEIFMKKMFNISEMEVNIIKNHL"
     gene            3539..3766
                     /locus_tag="PKK75_06600"
     CDS             3539..3766
                     /locus_tag="PKK75_06600"
                     /inference="COORDINATES: protein motif:HMM:NF019487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HNZ83305.1"
                     /translation="MAVISNQADSKLKLVLNAGVDINNKTVLKNKTLANVKAAVLNED
                     LYNLGVDISGLQDYTLANVVRYDEYELMEEI"
     gene            3811..4023
                     /locus_tag="PKK75_06605"
     CDS             3811..4023
                     /locus_tag="PKK75_06605"
                     /inference="COORDINATES: protein motif:HMM:NF022597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HNZ83306.1"
                     /translation="MAKKLVMSFLTALGGTSSMTIDAPRDDLTAAEVSTVMESIITKN
                     IFNTTKGDLAEIKSAEIITTTEEVLI"
     gene            4161..5495
                     /gene="dsdA"
                     /locus_tag="PKK75_06610"
     CDS             4161..5495
                     /gene="dsdA"
                     /locus_tag="PKK75_06610"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HNZ83307.1"
                     /translation="MEVKMKEELLIQYPELKNVSELNEVFWLNPRMVPYDKAASSINM
                     DIAAIDDAEKRLEKFAPLIEKVFPETIPSHGIIESPLQEISSMKSKLEELYKLKFSGK
                     LLLKMDSHLAIAGSVKARGGIYEVLMYAEKLAMKNGLLKEDDDYSILADQKFRDFFGR
                     YKIQVGSTGNLGLSIGITSAALGFNVTVHMSADAKQWKKDLLRSKGAKVVEYDSDFSK
                     AVEEGRKLSDQDPMSYFVDDENSMNLFLGYSVAARRLEEQLKNMNIVIDEAHPLFVYL
                     PCGVGGAPGGITYGLKSVYKDNVHCFFVEPTHAPSMLIGMATGLHNDISVQDLGIDGK
                     THADGLAVGRPSKLVGKMMDYVLGGVFTVDDYKLYNYMRYLNETENINIEPSACAAFE
                     GFIKFESSEEGKQYIKQHKLENIMQNAVHTAWATGGSLVPEEINRQFLATRL"
     gene            5699..6949
                     /gene="hisS"
                     /locus_tag="PKK75_06615"
     CDS             5699..6949
                     /gene="hisS"
                     /locus_tag="PKK75_06615"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNZ83308.1"
                     /translation="MKANLNPAKGMRDITPYEKEIRDYVEGVIVNTYKQSGFELFETP
                     VVENIENLTGSQGGENLMLIFKILKRGDKLNLEKEGLTEDDLSDLGLRYDLTVPLSRF
                     YCNNRTELLPVFKALQVGNVFRAERAQKGRYRSFKQCDIDIIGDGTQAAELELIATTA
                     KALTALNVKDFVVRFNDRRVLKAVILNCGFTEEEFDGVCIIVDKLDKIGIDGIKSEME
                     DKGYNKANAENLLEALNEINKKGTDCLNDYGVPKDVVSDVKKVLDESNAYATGKYDVV
                     FDFTLIRGMGYYTGSIFEITYKDLGYSIAGGGRYDEMVGNFIGEKIPAIGFSIGFERL
                     VNQLMEENFKVPDLDKAVLLYESGDRYIDVLAKAESIREEGYTVSIFEKAKKLGRQLN
                     QFQEYGYKKFAVYSGDETELKDLN"
BASE COUNT         2265 a         1268 c         1367 g         2113 t
ORIGIN      
        1 cccgtatagg tatcgtcaaa tcccgtcgga cttttcttat acatttcaaa ggtttttatt
       61 atatcataat cattcattgc ttcaacaaat gttctttcct tttcgcccag gaagtataga
      121 acatacaatt tgtttattga atacttggta aacatatttt cataaccgcc catggttaca
      181 gcaggtttag gattcttttc aatcataagc agatttggca gctcaacaac ctttgagttt
      241 tgacttaaag gcatagttag atttcttaaa taccttagca aggttgtatc cttatacaat
      301 gaaaagctgt aacttccaaa actgtaatct ctgaagtcaa ccccggatgt atccggattc
      361 ggcctaaagt ctacgtttaa aatggaggga atcttgttgt ctttttttaa atcctcataa
      421 atcaagaccc tgagcatatc cttgttatcg tttttaatat aataggcata tctgaaaaat
      481 ataatattag ctttggaatt atcactgccg gttaacttgt tccatttttc cagtgccccc
      541 atatacaggg tttcacttac gtcatatttg cttatcaatg taaaaatctg catcatttcc
      601 gcatccttca aatcatttct tgctatgaca ttagtcagaa tttcattggc aagttttgat
      661 ttttctgcat tgggatagct gccgaacata atcatgtaat aattggtgcc cacgatatta
      721 aaatatccgc ttgtgcgaag agactccatt gttttaacag catccatatc ataaaggtga
      781 ctatcctccg ggaaaatata acctccgaat gccaaatatt ccaaagtatc ctttgcctta
      841 ataaattttc tgtcattcgt atttgtagta tttaagtcat aattgccgaa ataggaatac
      901 atctcttcgc ccttgatggg cataaattca agcttagtga gataattgcc gccggtgctt
      961 gttttgatat ccgtataaac tcttcttatt agggaagcag attcagctct ggtaatgttt
     1021 gtgctgcctc tgaacgtatt attttcatag ccgacgatta ttcctgcatt cacaagcctt
     1081 tggatttcag aataatactt gttgtcagca ggcaggtctg taaatgtgag tggattgtca
     1141 aaaagtgctc ccttgctgaa ggcagcaatc aacgcagctg attcctctct ggtaatagcc
     1201 ctatcggggt aaaaattact tccggggaag atgtcattta aagtaaattt atttgttttt
     1261 agatattcat tcatagaaat aataaaagta taagaccaat gcttgtcgga catatctgaa
     1321 tatgacagat cgcccgtgta gatttccttc atttgatttt gcctgtaggc agtcctggat
     1381 aatatggcaa taaattctgc tcttgttata ttattttcag gtctgaaagt gaaatcacca
     1441 tatcccacaa aaaccttcat attattggaa gcaaaattaa tgtcgctttc agcccagtga
     1501 ttgaaagtat cataaaatac cgttcttgcc aaaacaggac gtgttgataa caaaacaaaa
     1561 gtaacaatta ataaaaatag ctttttaaat ttcctcaaat cgtgccatca cctcctttat
     1621 agatggttct taattatatt tacctccatt tcactaatat taaacatctt tttcataaat
     1681 atttcaatgc taaagttttc taaatcagaa aaattctgag cagtgttctt ctgaggcaga
     1741 taaattttta aattatttat tttatttgga tagtattcat aaacattttt tcctaccctt
     1801 ttaacctgac atttcaagta aaactcgaat atttccgaat tcaggtaaga aataaggtaa
     1861 tctacgggaa cgtcttttga aaaattattc atcaaataaa tatcagcact gcaataatat
     1921 gcttcatcat cataataaaa attattttgt tttgatttat aagggaatat tattttagga
     1981 ttatcatagt cagatttgct tctgccccat tgaagctcat accatttgcg caatccgctt
     2041 ttacactctc ttctgttcat cagcttatcc ttatatccgg acagatagtt tattgcattg
     2101 gaacaatcat tctcattttc aatcatatcg gaatataaga gatataaatt attatatttt
     2161 atatttttct ttgatatgtt gctgttttta atccattttc tgagcaggaa cctttctatt
     2221 ccatattttt cgataacttc ctctgttaaa atgaaagctt tgtcgcatcc tgtaataatc
     2281 ccctgcttaa tggtgcagac atccttgata taagtgttag agatgctgtc aattttttca
     2341 aaaagcttct catcctcatc cttaagaatt atccacccgg aatccttcaa cttgtcctgc
     2401 gcatattcat aggattcgaa aacatccttg tcttttttga cgtaggaaaa gatgcttttg
     2461 ttccaaaaat tagaaagtat tattattgcc ggacttacca tagccctttt aaaggcaggt
     2521 tcacccttat agtctattat tttgactata ttgaaatttt cctttatgaa gtttctcaat
     2581 ccttcagcat ataaggcttc cataaaatag cgtgatgtaa taaaggcaat gataccgtct
     2641 gtcttaagct cttccttgcc cttgttaaaa aagcagtaga aaacatcggc cttgtcgtaa
     2701 aatacaccgg aatacttttc atacaaaagt cttttatagg aggcgcttga atttttatgg
     2761 cccacataag ggggattgcc tattattatg tcaaacttat ccatattgat attatcaatg
     2821 agaaaatctc ccttgtagat attgaatttt atattatcaa gcccggtgtg aaaaatcagt
     2881 cctattttgc ttaaaaatat agaaaaatca tcaatgtcaa ttccaaagag catgttttcc
     2941 gctatatatc tcttatcaag ctcagggtgg gtactgctta tatatttgta taattcaatg
     3001 agaaaataac cgcccccgca ggaagggtcc aaaattctga cggagccgtc aattttttta
     3061 atagcgaata cttcgcaaag cattttgtct attatttcaa aaggagtata aacctgaccc
     3121 agggattttt tttccttgcc ggtaagcagg ctttcgtaga gtgaagcagg ataaaagctt
     3181 gaaactttaa tattgccttc atttatacct gtaaactctt caattctttt ctcagccttc
     3241 ttgtctttca ggtcaaaatt gctgtattcc ttgaagatcg atggattctt cacagggtat
     3301 tcatttttcg aattatataa ttctttgcat atcaaaaaaa ttacatactt aaccttgctt
     3361 atatagtcat gctcttcaat ttcattgatt atgcacttga tttcccttat cagcgacttg
     3421 tccataactg ccccttgaat tgcttataca tgtatacatt atcatgtttt gcgaatttaa
     3481 gcaatacatc cggcatctaa gggctatata gctttagaac aaaaggggag gtgatataat
     3541 ggcagtaatt tcaaatcaag ctgactctaa actaaagctg gttctcaatg caggagttga
     3601 catcaacaac aagaccgtct taaaaaacaa aacgcttgcc aatgtgaaag cagctgttct
     3661 aaatgaagat ttgtacaatc ttggagttga catttccggt cttcaggatt acacccttgc
     3721 aaatgttgta aggtatgatg aatacgaatt gatggaagaa atttaatgag ggattaagga
     3781 tggaattctt aaacagaaag gggtgataca atggctaaaa aattagtgat gtcattcttg
     3841 acagccctgg gaggcacttc ttccatgacg attgatgctc ccagggacga tttgacagca
     3901 gctgaagtaa gcacggttat ggagtcaatc ataacaaaga atatattcaa cactaccaaa
     3961 ggcgatttgg ctgaaataaa atctgcggaa ataattacta ctacagaaga agttttaata
     4021 taattagtgg ttaaactatg atataataca atcaataaaa ctaaggtccg ggggcacttt
     4081 agcgtgcccc cataaattcg gggacattcc tctgacctca gaggttcact tttaagaaga
     4141 ggtgtgtcgc cacacctata atggaggtaa aaatgaaaga agaactttta atccaatacc
     4201 cggaacttaa aaatgtatca gagttaaatg aagtattctg gctgaatccc aggatggttc
     4261 cctatgataa agctgcatct tcaataaaca tggatattgc agctatcgac gatgctgaaa
     4321 agcggcttga aaaattcgct ccccttattg aaaaggtatt tccagaaacc atcccgtccc
     4381 atggcataat cgaatccccc ctacaggaaa tcagcagcat gaaatccaag cttgaggaat
     4441 tatacaaact taaattttcc ggaaaactgc ttcttaaaat ggacagccat ctggcaattg
     4501 cggggagtgt taaagcaaga ggcggaatat atgaagtgtt aatgtatgct gaaaaactgg
     4561 ctatgaagaa cggattatta aaggaagacg atgattattc tattcttgcg gaccaaaagt
     4621 tcagggactt tttcggcaga tataaaatac aggtgggctc cacggggaac ttaggcttaa
     4681 gcataggcat aacatctgct gccttgggat tcaatgtgac agttcacatg tcagccgatg
     4741 caaaacaatg gaaaaaggac ctcttgaggt caaagggagc aaaggttgtg gaatatgaca
     4801 gcgacttttc aaaggcagtg gaagaaggcc gcaagttatc agaccaggac cccatgagct
     4861 actttgtaga cgatgaaaac tccatgaatt tattcttagg ctacagtgtt gcagccagaa
     4921 gactggaaga acaattaaaa aacatgaaca tcgttattga tgaagcccat cccttgtttg
     4981 tctacctgcc ctgcggtgtg ggaggagcgc ccggcggaat tacctacggg cttaaatcag
     5041 tatacaagga caatgtccac tgcttttttg tggaacccac ccatgcgcca agcatgctca
     5101 taggcatggc aacaggactt cacaatgata tttcggtcca ggatttgggg atagacggca
     5161 aaacccatgc agacggtctg gctgtgggaa gaccttccaa gctggtaggg aaaatgatgg
     5221 actatgtttt gggaggagta tttacagtag atgactataa gctgtacaat tatatgcggt
     5281 accttaatga aactgaaaat ataaatattg aaccctcagc atgcgctgcc tttgaaggct
     5341 ttataaaatt tgaaagctca gaggaaggga aacaatacat aaagcagcat aaactggaaa
     5401 acataatgca aaatgcagtc catacagcct gggcaaccgg cggaagcctg gtgccggaag
     5461 aaataaacag acaatttctg gcaacccggt tgtaagaaag aggggatagc ctaaaactgt
     5521 cccacatatc aatggaactg gggcactgtc acacaacaag tgaacgcatg ccgagagggc
     5581 agagtataaa actattgaaa gtaaatggtt ttatggtata atcaagagaa aaacaaacaa
     5641 atcaagggat agagagacag taaaagaccg tccccggtgt cccaagatag gaggcagaat
     5701 gaaagctaat ctaaaccctg caaagggaat gcgggatata accccttatg aaaaggaaat
     5761 ccgggactat gtggaggggg taatagtaaa tacatataaa caaagcggtt ttgaactttt
     5821 tgaaacacct gttgtggaaa atatagaaaa cttgacaggc agccagggcg gagaaaattt
     5881 aatgcttatt tttaaaattt taaaaagagg agataagctt aatttagaaa aagagggttt
     5941 aacagaagat gatttgtcag atttaggact caggtacgat ttgacagttc ctttaagccg
     6001 cttttactgc aacaaccgta ctgaactact ccctgtattc aaagcacttc aggtgggcaa
     6061 tgttttcagg gccgaaagag cccagaaagg aagataccga agcttcaagc aatgcgatat
     6121 agatataatt ggagacggaa cacaggccgc ggaattggag ctgattgcaa caacagccaa
     6181 agcccttacc gcccttaatg taaaggactt tgtggtgcgt ttcaacgaca ggagggtatt
     6241 gaaggcggtt atattaaact gtggtttcac ggaagaagaa ttcgacgggg tttgcataat
     6301 agttgacaaa ttggataaga ttggcataga cgggattaag agtgaaatgg aagacaaagg
     6361 ctacaacaaa gctaatgccg aaaatcttct ggaagcactt aatgaaatca ataaaaaagg
     6421 aactgactgc ttaaatgact acggcgttcc aaaggacgtg gtttccgatg taaaaaaggt
     6481 tcttgatgag tccaatgcct atgcaacagg taaatacgac gtggtatttg actttacatt
     6541 aataaggggc atgggatatt acacaggttc aatttttgaa ataacatata aagatttagg
     6601 ttattcaatt gcaggcgggg gaagatacga tgaaatggta gggaatttca taggtgaaaa
     6661 aatacctgcc ataggatttt caatcggttt tgaaagactg gttaaccagc ttatggagga
     6721 aaacttcaag gttccggatt tggataaggc agtgctttta tatgagtccg gggacagata
     6781 catagatgtt cttgcaaaag ctgaaagtat aagagaggaa ggctataccg tttcaatatt
     6841 tgaaaaggcc aagaaattag gcaggcagtt aaatcaattc caggaatatg gctacaagaa
     6901 atttgccgtt tacagcggag atgaaaccga acttaaagat ttgaattagt atataataaa
     6961 tgacgggtgt tttgtcaatt taatgaaggc aacaccccgt caggttgtgt gaa
//
LOCUS       DAOPUT010000076         6834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_11097_length_6834_cov_18.156365, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000076 DAOPUT010000000
VERSION     DAOPUT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11097_length_6834_cov_18.156365"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="PKK75_06620"
     CDS             complement(<1..1808)
                     /locus_tag="PKK75_06620"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNZ83309.1"
                     /translation="MEYFEYFKNNNGMELNSRQMEAASFERGNALVLSTAGSGKTTVI
                     IARAGRLLYENKCSRKILTITFSKMAANDMKERFACIGQDYEAKTEFSTIHAFSYKIA
                     RDFYRRKGINFGLLTNNYQVLSEILKNYYSKSYFSYVSEEEIENLSSAIGYANNMMIA
                     PEEYRDHGIEIKGFDKLYNDYKMYKSQNRLIDFDDMLVLSYKILSDSENYREQVLNAF
                     EYVQIDEMQDTSKIQHEIIKLITRDNLFMVGDDDQAIYSFRGSYPDFMLKFKDTYNEG
                     KIFYLDNNYRSDKYIVEGARNFIGINKNRYKKAIKTENKRENEINIIKVKTRADQAKL
                     ITDVVKEAGKGSENKTVGILFRYNMSAMIPANSFYENNIDFYIKEDKTKFFSNAVLYD
                     ILAFLNLAINPKDRDAFARIYYKSYTYFTKGMCKMVQDSKGDKSVFDILSGYMHFDYN
                     VYEKIKQFKTDINYINMMKPNNAVRFIKLDLEYINYLERMQEEGRYSLSNSLHILEIL
                     EEIGSYCSDINEFLKKLSNLRDVIKRASENRKANITLTSIHSAKGLEYDYVYMIDNME
                     DMNIDKNISADEYEKKLEEERRVFYVGMTRAKNKLN"
     gene            2144..3088
                     /locus_tag="PKK75_06625"
     CDS             2144..3088
                     /locus_tag="PKK75_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83310.1"
                     /translation="MKNKRSGLKRNKLITVFIAIIVIIAAITIAEKIVEPMGYTPPDY
                     KKTDITGILEKEELSDDDYKELFYQTGLGKTAVDEILKKDDEYKEQLRQTGLNEEAIE
                     EMLEKHVSGKEKILKFQENFFTRYNISRTKSAVIVNHEYLANEEGKLIYGFDLAPYEN
                     GYVLVTKATHSLGWRHGHAGIVTDAENEETLEAVLLGTDSMLQDIDKWRCFPSFMMIK
                     LKDVSQETLDEIAEFAKNNMLDVPYGLTVGVLSSKNPLPENIATTQCAHIVWYPFMQF
                     GYDIDSDGTWLVTPKDIANSGLFEIVQIYGVNPEEIWP"
     gene            complement(3245..3589)
                     /locus_tag="PKK75_06630"
     CDS             complement(3245..3589)
                     /locus_tag="PKK75_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83311.1"
                     /translation="MDFLYEKIAYLRGLADGLDVKEDTREGKLFNALMEVIEEIADSM
                     SDIVEDQDEVNEYLDLLDEDLSIVEEEVFGDLELYEDDDDDDYDFDYDDDDDEYDDDL
                     EYEEHSGLAEED"
     gene            complement(3600..4157)
                     /gene="efp"
                     /locus_tag="PKK75_06635"
     CDS             complement(3600..4157)
                     /gene="efp"
                     /locus_tag="PKK75_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNZ83312.1"
                     /translation="MITAGDFKKGITISWNNGLWQVIDFQHVKPGKGAAFVRSKLKNL
                     VTGAIREETFNPTEKFALARIETKDMQYLYNDGELYYFMDPETYEQIPLNYEQVEEAI
                     VYLKENDFAQMRFYEGSPFEINAPNFVELEIVETEPGFKGDTATGANKPATTETGAKI
                     SVPLFVEIGDRVKIDTRTGEYLSRV"
     gene            complement(4198..4626)
                     /locus_tag="PKK75_06640"
     CDS             complement(4198..4626)
                     /locus_tag="PKK75_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ83313.1"
                     /translation="MQNKGFTLIELVVTISIIFIVLASAALVIDMDMFYLEKMADEFA
                     MDVRYVQIQSVKDENASYRISIDADKGYYKILNFSVVEKTVKFKDRYQIGYSNHESSV
                     GFTHNGTPINSGTFTILDTRTNKTKQISIVPTTGRTLIKE"
     gene            complement(4815..5342)
                     /locus_tag="PKK75_06645"
     CDS             complement(4815..5342)
                     /locus_tag="PKK75_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ83314.1"
                     /translation="MKNKGFTMIELLVSLGISSIVIGLIMSFFVSTYKGYRTVRNDSE
                     MDFQAQYILNFMTGRIVDSDSMSFARLNTQNYSMIAVRSSGAEYPVNTVSFKYGEDNN
                     ENYVFAIRNNIISYGKGEKDMSPTVELGNYVDGIYISLLRDESFQDARAVKIKIVMKK
                     DGQTYEAFQTAYMRN"
     gene            complement(5498..6046)
                     /locus_tag="PKK75_06650"
     CDS             complement(5498..6046)
                     /locus_tag="PKK75_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83315.1"
                     /translation="MNNKGSTMVLLVIVIALVIVLGTSVLSVAAKQYEIKRVNMDSKQ
                     CFYMSETGLNEAYVKSCVLIDESIAKALQMAEDYLLINPLDLIQTEIIFINNYIIHIK
                     AKIKDRIEAAENPSVEVLNANALIFNDGLLTVILQSKYVNENNVDRITGVDLVISVPE
                     FDDVKAGLYDVRSYIEFQNWNN"
     gene            complement(6039..6425)
                     /locus_tag="PKK75_06655"
     CDS             complement(6039..6425)
                     /locus_tag="PKK75_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83316.1"
                     /translation="MKRLFNNKGLTLVEVIMTLGLLGAVICPLMNLLVLAQKINSEGQ
                     KDYRVIQTAQYYMEETRAMGGIDTEIFDYNSEGMCYERTVLDVLDDYSAEIRVIPASY
                     GLHYIEVDILSDGEVVESLGGSIVYE"
     gene            complement(6409..>6834)
                     /locus_tag="PKK75_06660"
     CDS             complement(6409..>6834)
                     /locus_tag="PKK75_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83317.1"
                     /translation="AEYENMNQDINLKSLYEENQDYLIKKVNELNIDREILQEEIISE
                     LIGISRKNNIELSSIKFSEAMPILSDDTGISMKVTVDFDSEFNKVLKFVDDVKNSCML
                     VSVEDISVLTIESGVHAAVNILFYALPMNILVQGHETVV"
BASE COUNT         2082 a         1501 c          999 g         2252 t
ORIGIN      
        1 atattcagtt tattctttgc gcgggtcata cccacataaa aaactctccg ctcttcctcc
       61 agcttttttt catactcgtc tgcagaaata ttcttatcaa tattcatatc ttccatatta
      121 tcaatcatat aaacgtaatc gtattccaag cccttggcgc tgtgaattga tgtaagcgtg
      181 atatttgcct tcctgttttc tgaagccctc tttattacat ccctcaaatt actcagcttt
      241 tttaaaaact cattaatatc gctgcagtat gaacctattt cctccagaat ctccaatatg
      301 tgaagggaat ttgacaggct gtaccgccct tcctcctgca ttctctccaa ataattaata
      361 tattccaggt ctaattttat aaaccttact gcattattgg gcttcatcat atttatataa
      421 ttaatatcag ttttaaactg cttgattttc tcatagacat tatagtcaaa gtgcatatat
      481 ccggacaaaa tatcaaaaac actcttatcc cccttgctgt cttgaaccat cttgcacatg
      541 cccttggtaa aataagtgta gcttttgtaa taaatccttg caaatgcatc cctgtccttg
      601 ggatttatcg ccaaattcaa aaatgcaaga atgtcgtaaa gaactgcatt gctgaaaaac
      661 tttgttttat cctctttaat ataaaaatca atattatttt cataaaaact atttgccggt
      721 atcatggcag acatattgta cctgaataat atgcctacag tcttgttctc tgaccctttg
      781 cccgcctctt taaccacgtc ggtaatcagc tttgcctggt ctgccctggt tttaaccttt
      841 ataatattga tttcattttc tcttttattc tctgttttta tagctttttt atatctgttt
      901 ttatttatcc cgataaaatt tcttgcacct tccacaatat acttgtcaga acggtaattg
      961 ttgtcaaggt aaaaaatttt tccctcattg taagtatcct taaacttaag catgaaatcc
     1021 ggataacttc ccctgaatga ataaatcgcc tggtcgtcat ctccaaccat aaacaaatta
     1081 tcccgggtta tcagtttgat tatttcatgc tgaattttgg atgtatcctg catttcatca
     1141 atctgcacat attcaaaggc atttaaaacc tgctccctgt aattttcaga gtcggacaat
     1201 attttatagg acaaaaccag catgtcgtca aaatcaatca gcctgttttg agacttgtac
     1261 attttataat cattgtaaag cttatcaaaa cctttgattt caattccgtg gtccctgtat
     1321 tcctccggag caatcatcat attattggca tatccgattg cagatgatag attctcgatt
     1381 tcctcctcac tcacatagga gaaatatgac ttggaataat aattttttag aatttccgag
     1441 agcacttgat aattatttgt aagcagtcca aaattaatgc cctttcttct gtaaaaatcc
     1501 cttgcaattt tataagaaaa ggcatgaata gtcgaaaatt cagtttttgc ttcataatcc
     1561 tgcccgatgc atgcgaatct ttccttcatg tcattggctg ccatttttga aaatgtaatg
     1621 gtaagaattt tcctgctgca tttgttttca tataaaagcc ttccggctct tgcaatgata
     1681 acagttgtct tgcctgagcc tgcagtggaa agaaccaggg catttcccct ttcaaaggaa
     1741 gccgcctcca tctgccggct gtttaattcc ataccattat tattcttgaa atattcaaaa
     1801 tactccatat accaccattt atatgttact ttgaatccgt tttaagatgc agtagcccga
     1861 ttttattcca ttgtgaactt cattccaccc agataagacc cttctactta actttactat
     1921 ttataacaag tatcccaata aactcatgaa ggcagtcaaa accattccga cagctaaaat
     1981 caaacaaatg gctcttgtaa ttttacttct tttatttatg ttcatttgaa tcctccaact
     2041 cattaatata ccatattata acacaaaatt ttcccctttt ccattgaaat ttttgcttaa
     2101 tatttatata ataataaaag tgaaattttt aaaggaatag attatgaaga ataaaaggtc
     2161 cggattaaaa cgaaataaac taataactgt atttatagca ataattgtaa taatcgctgc
     2221 aattacaatt gcagaaaaaa tagtcgagcc catgggctat actcctcctg actacaaaaa
     2281 aactgacatt acaggaatat tggaaaaaga ggagctgagc gacgatgact acaaggaact
     2341 tttttatcag acaggcctag gtaaaacagc cgtcgatgaa attttaaaaa aagacgatga
     2401 atacaaggaa cagctcagac agacaggtct caacgaagag gccattgaag aaatgctcga
     2461 aaaacatgta tcaggcaagg aaaaaatact caaatttcag gaaaattttt tcacccgata
     2521 caatatatca cggacaaaat ctgcggtaat cgtaaaccat gaataccttg cgaatgagga
     2581 aggcaaactg atatacggtt ttgacctggc tccctatgaa aacggatatg tattggttac
     2641 aaaagccacc cattcattgg gctggagaca tggacacgcg ggaatagtaa ccgatgcgga
     2701 aaacgaggaa accctggaag cagttttgtt gggaacggat tccatgctgc aggatattga
     2761 caagtggaga tgtttccctt catttatgat gattaaattg aaggatgtat cccaggaaac
     2821 cctggatgaa atcgctgaat ttgcaaaaaa taatatgctt gatgtcccct atgggctaac
     2881 tgtgggtgta ttaagcagca aaaatcccct cccggaaaac attgcgacca ctcagtgtgc
     2941 ccatattgtc tggtatccct ttatgcaatt cggatacgat attgacagtg acggcacctg
     3001 gcttgtgaca cccaaggata ttgcaaacag cggcctgttt gaaatagttc aaatatacgg
     3061 tgtgaatccg gaagaaatct ggccgtagca gctagaacgt ataatttggg aacattccat
     3121 caaagccatc tttcaaagag ttatattcct atgaaggtta gtccccatac ttgtataaat
     3181 tggattaagt cgcacaaaaa agaacattcc cttaaagaaa tgtcctcttc ttatgtaccg
     3241 gttgttagtc ttcttcagcc agaccgctgt gttcctcata ttctaaatca tcgtcatatt
     3301 cgtcatcgtc gtcgtcataa tcgaagtcat aatcatcgtc atcatcatct tcatataatt
     3361 ccaaatctcc gaatacttct tcttcaacaa ttgataaatc ttcatccagt aaatctaaat
     3421 attcatttac ctcatcctgg tcttcgacaa tgtcactcat tgaatcagca atttcttcaa
     3481 taacttccat cagtgcattg aacagtttgc cttctcttgt gtcttccttc acgtccagcc
     3541 cgtcagccaa acctctcaaa tatgcaatct tttcatataa aaaatccatg tcttacctcc
     3601 tatactcttg ataaatattc acctgttctg gtgtcaatct taactctatc ccctatttca
     3661 acaaaaagcg gtacagatat ttttgcaccg gtttcggttg ttgcaggctt gttagctcct
     3721 gttgcagtat cgcctttaaa acccggttct gtttcaacaa tttccagctc aacaaaatta
     3781 ggtgcattta tttcaaatgg actgccctca tagaacctca tctgggcaaa atcattttcc
     3841 ttcaaataaa caatcgcttc ctcaacctgt tcgtaattta gcggaatttg ttcatatgtt
     3901 tcaggatcca taaaataata taattctccg tcattgtaca aatactgcat atccttggtt
     3961 tcaattcgag ccagagcaaa tttctcggta ggattgaagg tttcttccct gatagcccct
     4021 gtcactaaat tttttaattt agacctgaca aaagcagctc cctttcccgg cttcacatgc
     4081 tggaaatcaa taacctgcca caatccatta ttccaagaaa ttgttatacc ctttttaaaa
     4141 tcacccgctg taatcatagc ttcctcctta tgtatattat aaccagcagt tacttcctta
     4201 ttccttaatt aacgtcctgc ctgtcgtggg aacaatggat atttgcttag ttttatttgt
     4261 ccttgtatcc aatattgtaa aagttcccga attaattggt gtaccattat gggtaaatcc
     4321 cacactgctt tcatggtttg aataccctat ttgatacctg tccttaaact ttactgtttt
     4381 ctcaacaaca gaaaaattta gtattttgta gtatccttta tcagcatcaa tgcttattct
     4441 atagctggca ttttcatcct tcacagactg tatctgcaca tatcttacat ccatggcaaa
     4501 ttcatcagcc atcttctcca gataaaacat atccatgtca ataaccagtg cggctgatgc
     4561 cagtacaatg aatataattg aaattgtaac aactaattca attaaggtaa atcctttgtt
     4621 ttgcatatcg cctaaaatct accataggtt tattttataa actatttggg tgtttgtcaa
     4681 tgaaattatt taatattggt cgtgacaagg gggtaaaatt ggacagggta ctgtcatgtg
     4741 acaagggacg ggggtcagcc tgaacacctg tttgaaacct catccgtgtc acactgaggt
     4801 cttgctctaa gaattcaatt tctcatatat gctgtctgaa aagcctcata tgtctgtccg
     4861 tcctttttca tgacaatttt tatttttacc gccctggcat cttgaaaact ttcatccctt
     4921 aataaagaaa tataaatgcc atccacataa tttcccagct ccacggtggg actcatgtcc
     4981 ttttcccctt taccgtagct gataatgtta tttctgatag caaaaacgta attttcatta
     5041 ttatcttctc catatttaaa ggaaaccgta tttaccgggt attcagcccc tgaacttctt
     5101 actgctatca tggaataatt ttgagtattt agtctagcaa aactcatgga atcggaatca
     5161 acgattctcc cagtcatgaa attcaatata tactgtgcct gaaagtccat ttccgaatca
     5221 ttcctcacag tcctgtaacc cttataagtg cttacgaaaa aagacatgat caacccgatg
     5281 acaattgagg aaatccccaa cgacaccaaa agttcaatca ttgtaaaacc tttgtttttc
     5341 atgactgccc ccctaacagc ctgagtgaaa cgaaagatcc cacaactaca tttttcagta
     5401 tgtttaataa ctgtaggtca ttcgtaatca gcaccaaaat ttttttgaat aataaacata
     5461 taattttgtg gttgaaactg cagaataaac tcccagatca attattccaa ttctgaaact
     5521 caatatagct tctcacatca tacaaacctg ctttcacatc atcaaattca ggcacactta
     5581 taaccaggtc tacccctgta atcctgtcaa cattattttc attgacatac ttagactgca
     5641 gtattacggt aagcagccca tcattaaata tcagagcatt ggcattcagc acttctaccg
     5701 aaggattttc tgcagcttca attctatcct taatttttgc ttttatatga attatataat
     5761 tgttaataaa aataatttct gtctgaatta agtccaaagg gttgattaat aaataatctt
     5821 cagccatctg caatgctttc gcaattgatt catcaattaa aacacaagac ttaacatagg
     5881 cttcgttaag ccctgtttca gacatataaa agcactgttt ggaatccata ttaaccctct
     5941 taatttcata ttgctttgct gccacactta aaacagacgt tcccaaaaca attaccagtg
     6001 caattacaat taccagaaga accattgtgg aacctttatt attcataaac tatactgccc
     6061 cccaaacttt caaccacttc cccgtcactt aaaatatcaa cctctatata gtgcagcccg
     6121 tagcttgccg gaatgactct tatctcagcg ctgtaatcat ccaatacatc caaaacagtc
     6181 ctttcataac acattccttc actgttgtaa tcaaaaattt ctgtgtcaat accacccata
     6241 gccctagttt cttccatgta atattgagca gtttgaataa ccctgtaatc cttttggcct
     6301 tcgctgttta ttttctgtgc caataccaat aaattcatca aagggcagat tacagcaccc
     6361 agtaatccca aagtcataat cacttccacc aatgtcagtc ccttgttatt aaacaaccgt
     6421 ttcatgacct tgcaccagga tattcatagg aagagcataa aacaatatat tcaccgctgc
     6481 atgaacaccg ctttcaatag tcaaaacgct gatatcctca acagaaacca gcatgcagct
     6541 gtttttaaca tcatccacaa atttaagcac cttgttaaat tcactgtcaa aatcaaccgt
     6601 taccttcatg ctaatccctg tgtcgtcaga cagaatcggc atggcttcgc tgaacttgat
     6661 actgctcagc tctatattat tcttcctcga aataccgatt aattcactta ttatttcttc
     6721 ctgtagaatt tctctatcta tatttaattc attaactttc ttaattaaat agtcttgatt
     6781 ttcttcatac aagcttttta gatttatgtc ctggttcata ttttcatatt cagc
//
LOCUS       DAOPUT010000077         6753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_11258_length_6753_cov_28.117796, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000077 DAOPUT010000000
VERSION     DAOPUT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11258_length_6753_cov_28.117796"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..269
                     /gene="rpsQ"
                     /locus_tag="PKK75_06665"
     CDS             15..269
                     /gene="rpsQ"
                     /locus_tag="PKK75_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNZ83318.1"
                     /translation="MERGNRKVRIGKVVSDKMDKTIVVATEKLVAHPLYKKRIKMTKK
                     YKAHDEENLCKIGDTVKIMETRPLSKEKCWRLVEILERAK"
     gene            300..668
                     /gene="rplN"
                     /locus_tag="PKK75_06670"
     CDS             300..668
                     /gene="rplN"
                     /locus_tag="PKK75_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNZ83319.1"
                     /translation="MIQTESRLRIADNSGAKEALVIRIMGGSVVKYGNIGDIVVCTVK
                     SATPGGVVKKGDVVKAVIVRTKSGVRRKDGTYIKFDENAAVIIKDDRTPVGTRIFGPI
                     TRELRDGKFMKIISLAPEVL"
     gene            681..986
                     /gene="rplX"
                     /locus_tag="PKK75_06675"
     CDS             681..986
                     /gene="rplX"
                     /locus_tag="PKK75_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNZ83320.1"
                     /translation="MHVKKGDKVVVIAGSDKGKIGKILENMPKKNRVVVENVNMQTKH
                     QKQTREVQQAGIITKEGAIDSSNVMLYCDKCKSGVRTEIKIQNDKKVRACKKCGTVL"
     gene            1011..1550
                     /gene="rplE"
                     /locus_tag="PKK75_06680"
     CDS             1011..1550
                     /gene="rplE"
                     /locus_tag="PKK75_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNZ83321.1"
                     /translation="MARLKATYKEQVVPALMEKFQYDSVMQAPKIEKIVLNMGVGEAK
                     ENQKFLDSAVEEMTLIAGQKPVVTKAKKSISNFKVREGMSVGCKVTLRGDHMYEFFDK
                     LVNIALPRVRDFRGVSKTAFDGRGNYALGIKEQLIFPEINYDKIDKIRGMDIIITTTA
                     NTDEEARELLSLMGMPFRK"
     gene            1564..1749
                     /locus_tag="PKK75_06685"
     CDS             1564..1749
                     /locus_tag="PKK75_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNZ83322.1"
                     /translation="MAKTSLKIKQQRKQKFSTREYTRCKICGRPHAYLRKYGICRICF
                     RELAYKGQIPGVKKASW"
     gene            1776..2174
                     /gene="rpsH"
                     /locus_tag="PKK75_06690"
     CDS             1776..2174
                     /gene="rpsH"
                     /locus_tag="PKK75_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNZ83323.1"
                     /translation="MVMTDPIADMLTRIRNANHAKHEFVDIPASKIKKEIANILLEEG
                     YIKGFDVIDDGKQGIIRVELRYQQNKERVITGIKRISKPGLRVYVGKEDTPRVLGGLG
                     IAILSTSKGIVTDKNARTQGVGGEVICYVW"
     gene            2198..2737
                     /gene="rplF"
                     /locus_tag="PKK75_06695"
     CDS             2198..2737
                     /gene="rplF"
                     /locus_tag="PKK75_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNZ83324.1"
                     /translation="MSRIGLKPVNIPANVEINIDGNNYAVVKGPKGKLEQQLSKSMKI
                     EVEGKKIKVSRPDDQKENRSLHGLTRTLINNMVTGVTEGYQKTLEIVGVGYRAQKQGN
                     KLVLNLGLSHPVEMEDPQGIETLVDGTNKIIVKGIDKQQVGNYAAIIRDWRRPEPYKG
                     KGIKYSDEVVRRKAGKTGK"
     gene            2749..3126
                     /gene="rplR"
                     /locus_tag="PKK75_06700"
     CDS             2749..3126
                     /gene="rplR"
                     /locus_tag="PKK75_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNZ83325.1"
                     /translation="MSVLLKKVDKNQKRKERHYTIRNKITGTPERPRLNIFKSTKHIY
                     AQVIDDAAGVTLASASTQDKELRDQVAGLTKKEAAKLVGKTVGERAKAKDINAVVFDR
                     GGYLYHGRVKLLAEGARESGLEF"
     gene            3141..3650
                     /gene="rpsE"
                     /locus_tag="PKK75_06705"
     CDS             3141..3650
                     /gene="rpsE"
                     /locus_tag="PKK75_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNZ83326.1"
                     /translation="MEQRGRIDASQLDVKEKVISINRVTKVVKGGRNFRFSVLVVVGD
                     ENGHVGIGMAKAVEIPDAIRKAIQDAKKNMIKVPLRETTVPHEMIGNYGAGRVLIKPA
                     KEGTGIIAGGPVRAVMELAGVKDIRTKSLGSSNPRNVVNATMEALKSLKSPDEVAKVR
                     GKAVEEILG"
     gene            3663..3839
                     /gene="rpmD"
                     /locus_tag="PKK75_06710"
     CDS             3663..3839
                     /gene="rpmD"
                     /locus_tag="PKK75_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNZ83327.1"
                     /translation="MATIKIKQTKSLIGRTPNQIKNIKALGLTKIGHVVEKEDTPQIR
                     GMIKKVDFMVEVIE"
     gene            3859..4299
                     /gene="rplO"
                     /locus_tag="PKK75_06715"
     CDS             3859..4299
                     /gene="rplO"
                     /locus_tag="PKK75_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNZ83328.1"
                     /translation="MKLHELKPNPGSTKNRKRLGRGTATGQGKTAGRGMNGQGSRSGG
                     VKGPAFEGGNMPLYRRLPKRGFTNIFGTQYAELNVEVLNQFEDGAEITPELLKSEGIL
                     KKQLDGVKILGNGVLEKKLTVKAHKFSKSAVEKIEAAGGKAEVI"
     gene            4300..>5519
                     /gene="secY"
                     /locus_tag="PKK75_06720"
     CDS             4300..>5519
                     /gene="secY"
                     /locus_tag="PKK75_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNZ83329.1"
                     /translation="MFETLKNAWKIPDLRKRMIFTLLMMVVYRLGAVIPVPGIDRAVV
                     ENMFQGTAGGLLDFFNMMSGGAFRDFTIFALNIYPYITSSIILQLLTIAIPKLEQFFK
                     AEDGKKKMAQWTRYITVILALIQATAYSIGFFNSAIIEKSFLSIATVIIVLTAGTAFL
                     MWLGELITEKGIGNGISVLIMIGIISQYPSDFGTVFYQFQTGAVNLLEILVFLVFAFF
                     IIVGVIAIQQGERKVPVQYAKRVVGRKMYGGQSTHIPMKVLMAGVMPVIFASTLLAIP
                     QTLAFFFPSMAAGVQKYFSVSASPGIYFYTLLNIVLIIFFTYFYTAVQFNPIEYSNTL
                     KENGGFIPGIRPGRPTADYLNKVLNRITIAGAIALAIIATIPTLVFSFTDLKINFGGT
                     SLLIVTGVALETMK"
     assembly_gap    5520..5619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5620..6189
                     /gene="map"
                     /locus_tag="PKK75_06725"
     CDS             <5620..6189
                     /gene="map"
                     /locus_tag="PKK75_06725"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNZ83330.1"
                     /translation="YNGFPFSICASVNEEVVHGFPSERQLREGDIISVDIGATFEGYV
                     GDSAKTFLVGQVDEEKRHLVETTRQCFYEGIKYARTSYRLSDISHAIQVYAESHGLSV
                     VRDYVGHGVGKKMHESPQVPNFGKPNKGPRLQEGMVLAIEPMINAGTYNVRVLENNWT
                     VVTVDGKPSAHYEHTVAITDGEPELLTIV"
     gene            6200..6493
                     /locus_tag="PKK75_06730"
     CDS             6200..6493
                     /locus_tag="PKK75_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HNZ83331.1"
                     /translation="MEGTTDLKIGQVVKSKAGRDKGRIFLICRVVDRQFVLVCDGDLR
                     KLNNPKRKKVKHLMIYNTVLTEFAEKLQENKNLNDAYVRKLLEPYALKEMEVE"
     gene            6497..6715
                     /gene="infA"
                     /locus_tag="PKK75_06735"
     CDS             6497..6715
                     /gene="infA"
                     /locus_tag="PKK75_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNZ83332.1"
                     /translation="MSKKDVIEVEGKVLEALPNAMFIVELQNGHQILAHISGKLRMNY
                     IKILPGDTVACELSPYDLTRGRITWRKK"
BASE COUNT         2446 a         1009 c         1581 g         1617 t
ORIGIN      
        1 aaggaggatt tactttggaa agaggcaaca gaaaagtaag aatcggtaaa gtagtcagtg
       61 ataaaatgga taaaacaata gtagttgcta ctgaaaaact tgtggcacat cctctttata
      121 aaaaaagaat taagatgacc aaaaagtata aagcacatga tgaagagaat ctgtgcaaaa
      181 taggtgatac agttaaaata atggaaacca gacctttgtc aaaagaaaaa tgctggagat
      241 tggttgaaat attagagaga gctaaataga ccctaagaca attgaaggga ggtaaaaaca
      301 tgatacaaac agagtcaagg ctgagaatcg cagacaattc aggagcaaag gaagcacttg
      361 taattcgtat aatgggcggc tcggttgtta aatacgggaa tatcggcgat atagttgtat
      421 gtacggttaa atccgcaaca cccggcggag ttgttaagaa gggtgacgtg gtaaaagcag
      481 ttattgtaag aactaaaagc ggtgtgagaa gaaaagacgg aacatatata aaatttgatg
      541 aaaatgccgc tgttattata aaagatgaca gaacacctgt ggggacacgt atatttggac
      601 ctataacaag agagttaaga gacgggaaat ttatgaaaat tatctcactg gcaccggaag
      661 tactttaagg aggtgtaaag atgcacgtaa aaaagggcga caaagtagta gttatagcag
      721 gcagcgacaa aggtaaaatt gggaaaattc ttgaaaatat gcctaagaag aacagagttg
      781 tggttgaaaa tgtcaatatg cagacaaaac accagaaaca aacaagggaa gttcagcagg
      841 caggaattat taccaaggaa ggagcaatag actcctcaaa tgttatgctg tattgcgata
      901 aatgcaaatc aggcgtaaga actgagataa aaatacaaaa tgacaaaaaa gtaagagcat
      961 gtaaaaagtg cggtacagtt ttataataat gccggaaggg aggtacgaat atggccagat
     1021 taaaagccac atataaggaa caggtagtgc cggcattgat ggaaaagttt cagtatgaca
     1081 gtgttatgca ggcaccaaaa atagaaaaaa tagttttaaa tatgggcgtt ggcgaagcta
     1141 aggaaaatca aaaattctta gacagcgccg tggaagaaat gacattgatt gcaggtcaaa
     1201 aacctgttgt aactaaagca aaaaaatcca tttccaactt taaggtaagg gaaggaatgt
     1261 cggtaggatg caaggtcacc ttgagaggag atcatatgta cgaattcttc gacaagcttg
     1321 taaatattgc cctgcctagg gttagagact tcagaggcgt atcgaagaca gcttttgacg
     1381 gcagaggaaa ctatgctttg gggataaagg aacagttgat ttttcctgaa ataaactacg
     1441 ataaaataga taaaataaga ggtatggata taatcataac aactacggct aatactgatg
     1501 aggaagcccg agagctttta agcttgatgg gaatgccttt tagaaagtaa gggagggagc
     1561 gaaatggcaa aaacatctct aaaaattaaa caacagagaa aacagaaatt ctccacaaga
     1621 gaatatacaa gatgcaaaat ttgcggaaga cctcatgctt atttaagaaa atatggtata
     1681 tgccgtatat gctttagaga attagcttac aaaggtcaga tacctggcgt taaaaaagcc
     1741 agctggtaaa attttagaaa aaggaggtta cttacatggt aatgacagat cctatcgcag
     1801 atatgcttac aagaataaga aatgctaatc atgcaaaaca tgaatttgtt gatattccgg
     1861 cttcaaagat taagaaggaa atagctaata tcttactgga agaaggatat attaagggct
     1921 ttgatgttat agatgacggg aaacaaggta ttataagggt tgaactcaga taccagcaaa
     1981 acaaggaaag agtgataacc ggtatcaaga ggatttctaa accgggactt agagtttatg
     2041 tgggcaagga agatacaccg agggttttag gcggcctggg catagctata ctttctacat
     2101 caaagggaat cgtaactgat aaaaatgcaa gaacacaggg agttggcgga gaagtaatct
     2161 gctacgtatg gtaaaataag gaataggagg tgccgatatg tcaagaatag ggttaaaacc
     2221 tgtaaatata cctgcaaatg ttgaaattaa tatagatgga aataattatg cagttgtaaa
     2281 aggaccaaaa ggtaaattgg aacaacagct gtcaaagtca atgaagattg aagtggaagg
     2341 taaaaaaatt aaagtttcaa gacctgacga tcaaaaagaa aacagatcac ttcacggact
     2401 tacaaggaca cttattaaca acatggtaac aggggttaca gaaggatacc aaaaaactct
     2461 tgaaattgtt ggtgttggat acagagcaca aaaacaagga aacaaactgg ttttaaacct
     2521 cgggctgtcc catcccgttg agatggaaga ccctcaaggt atagaaacat tagttgatgg
     2581 tacaaataaa attatcgtta agggtataga taagcagcag gtgggaaact atgcggctat
     2641 aatcagagac tggagaagac ctgaacctta caagggcaaa ggtatcaaat actctgatga
     2701 agttgtaagg cgtaaagccg gcaagaccgg taaatagcaa agaaaggagt gagtgtattg
     2761 cttaaaaagg ttgataaaaa tcaaaagaga aaagaaagac actatactat aagaaataaa
     2821 atcacaggta ctcctgaaag accaagattg aatatattta aaagcactaa gcacatatac
     2881 gctcaagtaa ttgatgatgc cgccggagtt acactggcca gtgcttcaac tcaggataaa
     2941 gagttaagag accaggtagc cggattgact aaaaaggaag cagcaaaatt agtcggcaaa
     3001 acagtaggag aaagagcgaa agcaaaggat ataaatgcag tagtttttga cagaggcgga
     3061 tatttatatc atggaagagt taagcttctt gctgaaggag caagagaaag cggactggaa
     3121 ttttaagaag gaggttaatt atggagcaac gtggacgtat agatgcaagc cagttggatg
     3181 tgaaagaaaa agttatcagc atcaaccgtg ttaccaaagt tgttaagggc ggcaggaact
     3241 tccgtttcag cgtgctggtt gtggttggtg atgaaaatgg tcatgttgga atcggaatgg
     3301 caaaagctgt tgaaattccc gacgctataa gaaaagcaat ccaggatgcc aagaagaata
     3361 tgattaaagt gccgctgaga gaaactacag tgccgcatga gatgattggc aactacggag
     3421 ccggcagagt tcttattaaa cccgccaaag aaggtacggg tataatcgca ggcggacctg
     3481 tccgtgcagt tatggaactt gcaggcgtta aagatatcag aacaaaatcc ttgggttcaa
     3541 gcaatccgag aaatgtcgta aatgcaacga tggaagcctt aaaatccctt aaaagccctg
     3601 atgaagtagc taaagtcaga ggtaaagcag tggaagaaat tttaggttaa gaggtgaaaa
     3661 taatggcaac gattaaaatt aagcagacca aaagcttaat cggcagaaca ccgaatcaga
     3721 taaaaaacat caaggctcta ggtttgacaa agataggcca tgtagttgaa aaagaagata
     3781 ctcctcaaat aagaggaatg atcaagaagg ttgactttat ggtagaggtt attgaataac
     3841 tacaaggagg tgttaaatat gaaacttcac gagttaaaac ctaatccggg cagtaccaaa
     3901 aatagaaaga gattaggaag aggtacagct acaggacaag ggaagacagc gggcagagga
     3961 atgaatggtc aaggctctcg ttcaggcgga gttaagggac cggcatttga aggcggaaac
     4021 atgcctcttt acaggagact tccaaagaga ggattcacaa atattttcgg aactcagtat
     4081 gctgagttga atgtagaagt tttaaatcaa tttgaagatg gagcggaaat tactcctgag
     4141 cttcttaaat cagaggggat attaaaaaag cagctggacg gagttaaaat tcttggaaac
     4201 ggcgtgctcg aaaagaagct tacggtaaag gcacataaat tcagcaagtc agccgtagaa
     4261 aagattgaag ctgccggcgg aaaagctgag gtgatttagt tgtttgaaac tttaaaaaac
     4321 gcttggaaaa tacctgattt aagaaaaaga atgatattta ctctgttaat gatggtagtt
     4381 tatagattag gagcagtaat acctgttcca ggtattgaca gagcagttgt agaaaacatg
     4441 ttccagggta ccgcaggtgg tcttttggac ttcttcaaca tgatgagcgg cggtgctttt
     4501 agagatttta caatatttgc cttaaatata tatccataca ttacgtcttc aattattctt
     4561 cagctgctga caattgctat tccaaaactg gagcaattct tcaaggcgga agacggaaag
     4621 aaaaagatgg ctcaatggac cagatatatt acagttatat tggcattgat tcaagctacc
     4681 gcatacagta tagggttctt taattcagct ataattgaaa agagcttctt gtcaatagca
     4741 actgtaataa tagtcttaac ggcaggaaca gctttcctta tgtggctggg cgagctgata
     4801 acggaaaaag gtatcggaaa cggtatatca gtactcataa tgatcggtat tatatcccag
     4861 tatccatctg attttggaac ggtattttac cagttccaga caggagccgt aaatttattg
     4921 gaaatattag tattcctggt atttgctttt ttcataatag ttggcgtaat agccattcag
     4981 cagggagaaa gaaaagtacc tgttcagtat gctaaaagag ttgttgggag aaaaatgtac
     5041 ggcggacaaa gcacccacat tcccatgaaa gtgttaatgg caggagttat gcccgtaata
     5101 ttcgcttcaa cacttttggc aataccacag acactggcat tcttcttccc gtcgatggca
     5161 gcaggagttc agaaatattt ctcggtatca gccagtcccg gtatatattt ttacacattg
     5221 ctgaatatag tgctgataat attcttcaca tatttttata cagcagtaca attcaatcca
     5281 attgaatact caaatactct taaggaaaat ggaggattca tacccggaat aagaccggga
     5341 agacctactg cagattattt gaacaaagta ctgaacagaa ttacaatagc aggagcaatt
     5401 gcacttgcaa taatcgcaac aatacctaca ttggtattct catttacgga tttgaaaatc
     5461 aatttcggag gaacatcact tttgatagta acaggtgttg ctcttgaaac tatgaagcan
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ataacggatt tcccttttcc
     5641 atatgcgctt ctgttaatga agaagtagtg catggatttc cttcagaaag gcagttaagg
     5701 gaaggcgata taataagtgt ggatattggc gcaacatttg aaggatatgt gggtgacagc
     5761 gctaagactt tcttagtggg tcaagtggat gaagaaaaaa ggcatttggt tgaaacaacc
     5821 agacaatgct tttacgaagg cataaagtat gccaggactt cataccgcct gtctgatata
     5881 tcacacgcaa tccaagttta tgcagagtct catggtttat cggtagtaag agattatgtt
     5941 gggcacggag tagggaaaaa aatgcatgaa tcaccgcagg tacctaactt cggaaaacca
     6001 aacaagggtc cgcggctgca agaaggaatg gtccttgcta tcgagcctat gataaatgca
     6061 ggaacgtata atgtaagggt tttggaaaac aactggactg tggtcactgt tgacggcaaa
     6121 ccatctgctc attatgagca tacagttgca ataacagacg gggaaccgga gctgttaact
     6181 attgtttagg ggtgggctga tggaagggac aacagatttg aaaatcggcc aggtagttaa
     6241 gtcaaaagca ggcagggata agggaagaat atttcttata tgccgggttg ttgacaggca
     6301 atttgtgctg gtatgtgacg gggatttaag aaaattaaat aaccccaaaa gaaagaaagt
     6361 gaagcattta atgatttata atacagtatt gacagaattt gcagaaaaat tacaagaaaa
     6421 taaaaatcta aatgatgcat atgttaggaa attattggag ccgtatgcat taaaagaaat
     6481 ggaggttgag tgatcaatgt ccaagaaaga tgtaatagaa gtcgaaggca aggtcctgga
     6541 agcacttcca aatgcaatgt tcatagtcga gcttcagaac ggacaccaga ttttagcgca
     6601 tatttctgga aaactgagga tgaactacat taaaattctt cccggagata cagtagcttg
     6661 tgaattatca ccatatgatt tgactagagg aagaataaca tggaggaaaa agtaaggagg
     6721 tattacaatg aaagtaagac catcagtaaa acc
//
LOCUS       DAOPUT010000078         6393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_12086_length_6393_cov_19.279268, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000078 DAOPUT010000000
VERSION     DAOPUT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12086_length_6393_cov_19.279268"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="PKK75_06740"
     CDS             <1..114
                     /locus_tag="PKK75_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HNZ83333.1"
                     /translation="CLSPGEKPAAVFALVEGDEVVAAYEYCNLHGLWKKEI"
     gene            complement(381..2333)
                     /gene="metG"
                     /locus_tag="PKK75_06745"
     CDS             complement(381..2333)
                     /gene="metG"
                     /locus_tag="PKK75_06745"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNZ83334.1"
                     /translation="MNKGKYYLTTAIAYTSAKPHIGNTYEIVLADSIVRFKKLNGYDV
                     YFQTGTDEHGQKIQLKAEEAGKSPQAFVDEVAADIKRIWDLMNTTYDKFVRTTDPEHV
                     KTVQKIFKKLYDQGDIYKGYYEGWYCTPDEAFFTDAQLVDGKCPDCGRDVYMAREEAY
                     FFKMSSYADRLMKHIEENPKFIQPESRKNEMVNNFIKPGLQDLCVSRTSFDWGIPVTF
                     DEGHVVYVWIDALSNYITFLGYDTDGNHGDLYKKYWPADVHLIGKDILRFHTIYWPIL
                     LMALDVELPKQVFGHPWLLVGEGKMSKSKGNVIYADDLVDLFGVDAIRYFVLHEMPFA
                     SDGTLTYELIIERINSDLANILGNLVNRTIAMTNKYFEGVISAPTAPEPIDDELIKLA
                     LETALKVEKKMDELKVSDSIDEIFTLLRRSNKYIDETQPWILGRDESKKERLGTVLYN
                     LLESIRIAAALLEPYLPETSGKILKQLNTGKKDWDSLLQFNGMVPGQKVGTPEILFAR
                     IDTAKKIEEINNINEAKHSKEPAKTKETPEKPKDVNYATMEDFKKLDLRVAEIIKVER
                     HPNADKLLVFQLKIGEETRQVVSGISQWYKPEDLVGKKVVMVYNLEPVTLRGVESNGM
                     ILSAEKGKKLSVVSTLEDMPGGSTIS"
     assembly_gap    2350..2449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     misc_binding    complement(2469..2702)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2755..2976)
                     /locus_tag="PKK75_06750"
     CDS             complement(2755..2976)
                     /locus_tag="PKK75_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83335.1"
                     /translation="MDILIGLFLFIVLIGAALKVGVVLLNVIFTFFGAIIGFIVILAL
                     IPLGIGLLLIPAIIIGIIVAIVKCIKLIF"
     gene            complement(3188..4432)
                     /locus_tag="PKK75_06755"
     CDS             complement(3188..4432)
                     /locus_tag="PKK75_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNZ83336.1"
                     /translation="MKTEFFINNRRKFAEKMQDYSAAVFFSGKAPRESADQKYSFSVD
                     RNFFYLTGIDREDMVLVISKTGGRTEETLYIPPVDEHFEKWYGILMRKEEALEASGIK
                     TLENRNDFLSKFSGRLSSVDRPDNVYIFSYITDADEPYDCYRSLAHWMRAQYPAVNIK
                     NSLDIMIELRSSKSEEEVEEIKTAIGHTREALEFVMKSLKPGMFEYQARADFEYQLML
                     RNSRPSFATIAASSGNAVILHYVDLQRKMQEGDMILLDLGALSNNYASDITRTYPVKG
                     KFTKRQKDIYKVVLEAQNVAVEAMHPGASETDVNNKVKEYFAKELKVLNLIKDDSEVD
                     KYYYHSIGHPLGLDVHDLRRRDKTIQENNVYTVEPGLYIKEEGTGIRIEDNVWVTKKG
                     IVNLSNDIIKSVDDIENFMNRR"
     gene            complement(4505..5980)
                     /gene="glpK"
                     /locus_tag="PKK75_06760"
     CDS             complement(4505..5980)
                     /gene="glpK"
                     /locus_tag="PKK75_06760"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNZ83337.1"
                     /translation="MEKKYVLALDQGTTSSRAILFDRNGRIVSMSQKEFTQIYPAPGW
                     VEHDPLEIWETQINAAKEAIKDINVSEIACIGITNQRETTILWDKNTGEPVYNAIVWQ
                     SRQTSDICDELKKSGLEPYIKENTGLVIDAYFSGTKIKWILDNVQGVREKAEKGHILF
                     GTVDTWLMWNLTGGKVHITDYSNASRTMIYNIKTLKWDEKILDILNIPHEILPEVRQS
                     SETYGNTKMEIFGTEILLSGIAGDQQAALFGQLCFQEGMVKNTYGTGCFMLMNTGEKP
                     VMSRKGLITTIAWGLNGRVEYALEGSIFVAGAAIQWLRDELKLIHDAADSEYFANKVG
                     DTKGVYMVPAFTGLGAPYWDMYARGAIVGLTRGAGRNHIIRAALESIAYQTKDVIEAM
                     IDDSGINLTALKVDGGASANNFLMQFQSDILNVNIERPEITETTALGAAYLAGLAAGF
                     WKSKGEVIQNWNMDKRFKPAINDDERDKLYSGWKRAVERVR"
BASE COUNT         1677 a         1404 c         1024 g         2188 t
ORIGIN      
        1 tgcttgtctc cgggagaaaa acctgcagct gtatttgccc tggttgaagg cgatgaagtt
       61 gttgcagctt atgaatactg caatcttcac ggactctgga agaaagaaat ataattgtta
      121 caaggtttat aagtctctcc ttaatgattt ttttataaaa aaggatttga atcccgattc
      181 aaatcctttt taaaatattt attgaggggt catcttttat ttatatgggg ttcgcctttt
      241 attcatacag gcttcgtcgg gcatcacgca gtctcattca ccaaaatatt tgctccttcc
      301 gtcgcatatt ttgggaattc gttgcgaatg acctaccgct atttttctct cccttcggtc
      361 agaaaaatag ggttagggca tcatgatatt gttgaaccgc cgggcatgtc ttccaaggtg
      421 gataccactg aaagcttctt ccctttttct gccgataaaa tcatgccgtt ggattcaacc
      481 cctctcaggg ttaccggctc taaattataa accattacca cttttttacc caccaggtcc
      541 tcaggtttat accattgtga aatgcctgaa accacctgtc ttgtttcttc gcctattttc
      601 aattgaaaca caaggagctt atctgcgttt ggatgtcttt caaccttgat tatttctgca
      661 actctcaggt caagcttctt aaaatcctcc atggttgcat agttgacatc ttttggcttt
      721 tccggtgttt ctttcgtctt tgcaggttct tttgaatgtt tagcctcatt tatgttattg
      781 atttcttcaa ttttctttgc agtatctatt cgtgcaaata aaatttcagg tgttcctacc
      841 ttttgtcccg gtaccatgcc gttaaactgc aggagggagt cccagtcttt tttgcctgta
      901 ttaagctgct ttagtatttt cccggatgtt tccggcagat agggttccag aagcgctgct
      961 gcaattctta tggattccaa aagattgtat aaaacagtcc ccaatctttc ttttttgctt
     1021 tcatctctgc caagaatcca gggctgtgtt tcatcgatgt acttgttgct tcttcgaagc
     1081 agggtgaata tttcatcgat ggaatcggag actttaagtt catccatttt cttttcaacc
     1141 ttcagggctg tttcaagagc aagcttaatt aattcatcat ctataggctc cggcgctgta
     1201 ggagctgaaa tcactccttc gaaatattta tttgtcattg ctattgttct gttaaccaaa
     1261 ttgcctaaaa tattagccaa atctgaattg attctttcta ttatcaattc ataagtcagt
     1321 gttccatcag aagcaaaggg catttcatga aggacgaagt acctgattgc atccactccg
     1381 aacaagtcta ccaaatcatc agcataaata acatttccct ttgatttgct catcttgcct
     1441 tcgcccacca acagccaggg atgaccgaaa acctgcttgg gaagctctac atccaatgcc
     1501 attaaaagta taggccaata aatagtgtgg aatcttaaaa tatccttgcc gattaagtgt
     1561 acatctgccg gccagtattt cttgtaaaga tcaccgtggt ttccatcagt atcatatccc
     1621 aggaatgtga tatagttgct caatgcatct atccaaacat atacaacgtg gccttcatca
     1681 aaggtaacgg gaatacccca gtcaaaggat gttcttgata cgcacaaatc ttggaggccg
     1741 ggcttgataa aattgttaac catttcattt tttcttgatt ccggctggat aaacttagga
     1801 ttttcttcaa tatgcttcat caatctatcc gcatagctgc tcattttaaa gaaatatgcc
     1861 tcttctcttg ccatataaac atctcttccg cagtcagggc acttgccgtc tactaactgg
     1921 gcatccgtaa aaaacgcttc atcaggagtg cagtaccatc cttcgtagta gcctttgtaa
     1981 atgtctccct ggtcgtagag cttcttgaat attttttgca cagtcttaac atgttcgggg
     2041 tctgttgttc ttacgaattt atcataagta gtattcatta aatcccaaat ccttttaata
     2101 tcagcagcta cctcatcaac aaatgcctgt ggtgacttgc ctgcttcctc agccttaagc
     2161 tgtattttct gtccgtgctc atctgttcct gtttgaaaat atacatcata tccgttcaat
     2221 ttcttgaatc ttacaatact gtcggcaaga actatttcat atgtatttcc tatatgaggt
     2281 ttggctgatg tatatgcaat tgcggttgtt aaataatatt tgcctttatt catgtatttt
     2341 cctccttgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aacgcctctt
     2461 agcataagct aagaggcgaa tagcgcgtta ccactctttt tttgcacata tttcacaaca
     2521 tgtgcctcaa caggttcaat taaaccttgt attataacgt atactaacgt cgtaccctaa
     2581 ctcctgtgct caggtacaaa gctccaaggc catgttcgtt ttaaaaccaa tccccggctt
     2641 ccaccatgcg ccggctctct atatcatgat tttaaaacta ctcttctttt catcgcttaa
     2701 gttattatgt attttcaata gataaactat acataaataa cttatttttg tcaactaaaa
     2761 aattaattta atgcatttta ctatagcgac aattatcccg attattattg ccggaatcag
     2821 caaaagtcct attccaagag ggattaatgc aagaatcaca ataaaaccga ttattgcccc
     2881 aaagaatgtg aagataacgt taagcagtac aacaccgact ttcaacgctg ctccaattaa
     2941 tactataaat aaaaacaagc caataagtat atccatttct acctccatat tttaacttgt
     3001 ataagatttc aaatcatttg tcatgcagga atgtcttatt aaagaatatc cctatattta
     3061 catagtacac ataaataatt caaaatcgat taatgctaaa ttaaaaaatg attagaaatg
     3121 aaggttctgc agttttacac acaattttat ttgtacgcaa actcaatata agtttgccgc
     3181 cggtcaacta tctcctgttc ataaaatttt caatgtcatc gactgacttg attatatcat
     3241 ttgataagtt aactattcct ttttttgtaa cccacacatt atcttcgatt cttatgcctg
     3301 ttccttcttc ttttatatat agtcccggct ccacggtata aacattgttt tcctggattg
     3361 ttttgtctct tcttcttaaa tcatgaacat caagccccag aggatgacca atgctgtgat
     3421 aataatattt gtcaacctcc gaatcatcct tgattaaatt aagcactttt aattccttgg
     3481 cgaaatactc cttaacctta ttgtttacat ctgtttcgga tgctcccggg tgcatggctt
     3541 caactgcaac attttgtgct tccaaaacca ccttgtaaat atccttctgc ctctttgtaa
     3601 atttaccttt tacaggatat gtccttgtaa tgtcggaggc ataattattt gacagtgcac
     3661 caaggtcaag aagaatcata tcgccttcct gcattttacg ctgcaaatca acataatgca
     3721 atattactgc attacctgat gaagcagcaa ttgtggcaaa gcttggtctt gaatttctca
     3781 gcatcagctg gtactcaaaa tctgcccttg cctggtattc aaacatgccc ggctttaaac
     3841 ttttcattac aaattccaaa gcttccctgg tatgtcctat ggcagtttta atttcttcaa
     3901 cttcttcctc tgacttgctg cttctcagct caatcataat atccaatgaa tttttaatat
     3961 ttactgcagg gtattgagct ctcatccaat gagctaaact tctgtaacaa tcataaggct
     4021 catctgcatc tgttatgtat gagaaaatat agacattgtc aggtctgtca acagacgaca
     4081 gcctgccgga aaactttgaa agaaaatcat ttctgttttc aagagtttta atgcctgatg
     4141 cctcaagggc ttcttccttt ctcataagga tgccgtacca tttttcaaaa tgctcatcaa
     4201 cggggggtat gtacagtgtt tcttctgtcc tgcctcctgt tttgctgatt accaaaacca
     4261 tatcctctct gtctattccc gtaaggtaaa agaaatttct gtcaacggaa aatgaatact
     4321 tctggtcagc actttctctg ggtgcctttc ctgaaaagaa tacagctgca gaataatcct
     4381 gcattttttc tgcaaatttt cttctgttgt ttatgaaaaa ttctgttttc atttattcct
     4441 ccatatgttt tgggacattt ctccgaaaga ctttccctga agaagaggtc tggtttgtcc
     4501 ccattcatct gactcgttca accgcccttt tccagccact gtataattta tctctttcat
     4561 cgtcatttat tgccggttta aaccttttat ccatattcca attttggatc acttcaccct
     4621 ttgacttcca aaatcccgca gcaagacctg caagatatgc tgcgccaagg gcagttgttt
     4681 ctgttatctc aggtctttca atattaacat taagtatgtc tgactgaaac tgcatcagaa
     4741 aattatttgc agatgctcct ccgtcaacct ttaatgctgt taaattaatt cctgaatcat
     4801 caatcattgc ttcaattaca tcctttgtct gatatgcaat ggattcaaga gctgccctta
     4861 taatatgatt cctgccagca ccccttgtga gtcctacaat cgctcccctt gcgtacatat
     4921 cccagtatgg agccccaagc cccgtaaatg caggaaccat atagactcct tttgtgtctc
     4981 caaccttatt tgcaaaatat tctgagtcag ctgcatcatg tatgagctta agctcgtccc
     5041 tgagccactg tattgctgct cctgccacaa atatggaccc ctccagggca tactcaaccc
     5101 tgccgtttaa accccatgca attgtggtaa taaggccctt gcgggacata accggctttt
     5161 ctcccgtatt cataagcatg aagcatcctg tgccgtaagt attttttacc attccctcct
     5221 gaaaacaaag ctggccgaaa agagcagcct gctggtctcc tgcaattccc gacaggagta
     5281 tttcagtgcc gaaaatctcc atctttgtat tgccgtatgt ttctgatgac tgcctgactt
     5341 ccggcaatat ttcatgaggt atgttcaata tgtcgagtat cttttcatcc cacttcagag
     5401 ttttaatatt gtaaatcata gttcttgaag cattggaata atcggttatg tggaccttcc
     5461 cgcctgtgag attccacatg agccaggtat ccacggtccc gaataaaata tgaccctttt
     5521 cggctttctc ccttactcct tgtacattat ccaaaatcca ctttattttt gtccctgaaa
     5581 aataagcatc gataaccagt cccgtatttt ctttgatgta gggctctaag ccggacttct
     5641 tcaactcatc gcagatatct gaagtctgcc tgctttgcca gactattgcg ttgtaaacag
     5701 gttctcctgt atttttatcc cagagaattg tggtttctct ttgatttgta attcctatgc
     5761 atgcaatttc tgaaacattt atatccttta tggcttcctt tgcagcattt atctgggttt
     5821 cccatatttc caaagggtca tgctcaaccc atccgggagc aggataaatt tgagtgaatt
     5881 ccttttgaga catgcttaca attcttccat ttctgtcaaa aagtattgct cttgaacttg
     5941 ttgtgccttg atctaaggca agaacatatt tcttttccat aactctcccc tttccaaccg
     6001 ggacactgtc cccctgatat atgagattac gcagctccca taatctttcc tttttctttt
     6061 ttattgtttt tgtccttaag tgacttgttt ccttataatc tcagtctgaa ttgttatgta
     6121 aaccctttct tcttactatt gtacaccctt tttattcaat tttaaaccct tattttctcc
     6181 catatattgt tattaatttg gcaaagtaaa aattatttgt cactatctac ataattcgtg
     6241 ttgaatattt aacaataata ctatatcatt aaggtaagta taaaaattag gaggatttta
     6301 tgaaaagatt tgtatttgtg ttaagcttgg tattgctctt ggcattatca tctgtaacag
     6361 cttttgcaga tgaagttgtc ataataaata tcg
//
LOCUS       DAOPUT010000079         6288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_12359_length_6288_cov_16.601316, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000079 DAOPUT010000000
VERSION     DAOPUT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12359_length_6288_cov_16.601316"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..431
                     /locus_tag="PKK75_06765"
     CDS             75..431
                     /locus_tag="PKK75_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HNZ83338.1"
                     /translation="MNYVMSFVIGGLICTAGQIIIDTFKKNNAYLLVLLVVTGCILGF
                     FGVYDKLVEIGHSGATVPLLGFGNSLAKGAMEEAAGKGFMGALSGGVIRTAPGVAAAL
                     IFGYTVAVIFNPHGDK"
     gene            complement(582..>3407)
                     /locus_tag="PKK75_06770"
     CDS             complement(582..>3407)
                     /locus_tag="PKK75_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNZ83339.1"
                     /translation="VREGDPTAQGASTITQQLVKNLYLTNEKSYERKIKEIYLAIKVE
                     RVLSKEQILENYLNTIPLGQSAYGVQTAAYTYFSKDVSDLTLAESCLLTGAAKGPSFA
                     PYNRYNLDSLNEIPEEDIVGYVFIGSVQYACVYNQNAIDRQHAILNRMLELEYINQEE
                     YDAAMAEDMRAALNPGQTKIEGISSTPMDYVKEKVVEDLMAELNMTYEEAESYLYKGG
                     LTITSTIDVNIQKSLEQSYNNFSVLYLGAEPSGDKPIAQDWRYFRWSGGEGDGMLDSA
                     LNILNENGQLIFFARENIMDDENSIYLNPDEFSTDENGNLVINSKKFDIYSSTIDIVD
                     AYTIDDGMNFVSHTIGALNVGNNYEVIEKKGSKGSFKIPKSYLDKNPGMFNIGSDGIL
                     RIPEGYFFFQEKGIVQPQSATVIMDYRTGKIKGMIGGRQIEGSKTFNRATDAARQPGS
                     TIKPLSVYLAALDLGYSAAYPIDDLPRYNQSGDRWPKNWYEHRSIKYWGIQTLRKSIE
                     QSINTNAVTMLDTIGFDAAINSLTKLGLIDQNNPENDTFVSPQEGPYYNDVNLASLAL
                     GGLTKGFSPLGMTAAYGAIANDGIYVEPIVYTHVVNSRDETILENIPETHIVVSPEVA
                     SLMKDILRTTVNPGLSYRARLPQELGIEVAGKTGTTQANGDFWFVGFSQYYVGGIWVG
                     NDNVQMKLSGDSGNNARLWSGIMTPIHQGLPPASFTRNPNLVPMSVCSQSGKLPGDLC
                     AQDQRGSRVITEYFIPGTQPTETCDVHVKAEICTSSNMLKSIYCPGNLVEERVFVVRD
                     PLYDPEAKETNYEAKKLYQQILEDRIIFTLEELQQIYGTQMSVDENGQITHVNEIEVE
                     KLGFSGLLTEDFQYQIPTRTCYYHTKWHYDQWLNEENNGGVNGENGNNGNGDTLEDII
                     DDIIDEFNDSEEDSEKPIDEILESVEN"
     assembly_gap    3408..3507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3879..5141
                     /gene="hflX"
                     /locus_tag="PKK75_06775"
     CDS             3879..5141
                     /gene="hflX"
                     /locus_tag="PKK75_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNZ83340.1"
                     /translation="MEKERCLLVAVQVAGEEENDMEELAQLVETAGGETVSSIVQNRQ
                     SIDNRFYAGQGKIEEIANYVKELDIDTIVFNDELSGSQIRNIEEIVDAKVIDRTNLIL
                     DIFAKRALTKEGQLQVELAQLKYSLPRLIGVNKNLSRLGGGIGTRGPGEQKLEIDRRR
                     IKEKITDIQNQLEVLEKVRETKRKKRMKDQIPIVSIVGYTNAGKSTLLNALMDNEYRE
                     EGENKKVFAHDMLFATLDTELRRVRLPGGRSVVFSDTVGFIKKLPTQIVEAFKGTLEE
                     IKYADLIIHLIDINDENLSAHKETTIELIDKMTGMEIPVLTVYNKVDKILDEKISVLN
                     NGIIYISAKYNYNINMLLEAVDNIINGKKSFYDLKIPNSLLKTYYWLYENRTTENIEF
                     EGDGVSLRALLYENEKDEYKNYMGVIDE"
     gene            5134..5778
                     /locus_tag="PKK75_06780"
     CDS             5134..5778
                     /locus_tag="PKK75_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNZ83341.1"
                     /translation="MSNYKSIHKEGRDELIINKSRFIGTACPVETEDEALEFIDKVKR
                     EFKDATHNVYAYVIGENSNIQRYSDAGEPSGTAGMPVLNVINQENIKNAAVVVTRYFG
                     GVLLGAGGLVRAYTKGSKIALESGIIVDKKLFYDVSFKIDYTLLGKMDNELVKNNFIL
                     VDRIYEDRVLIKLIAENENLDNIGALVGEITSGKAEISVGQAAYYSVKNGKIVE"
     gene            5789..>6288
                     /locus_tag="PKK75_06785"
     CDS             5789..>6288
                     /locus_tag="PKK75_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ83342.1"
                     /translation="MSGHSKWSNIKNRKGKQDALKGKIFTKLARAITVAVREGGAEPG
                     YNSTLATAIEKAKAQNMPNDNIDRAIKAGLSAASGENFETITYEGYGPSGTAFMVQCL
                     TDNKNRTAADVRHYFSKYNGNLGTTGCVGYLFDKKGVISIESEGVSEDELMMAALETG
                     AEDFEA"
BASE COUNT         1928 a         1114 c         1298 g         1848 t
ORIGIN      
        1 cctactgcga ctctccaggg tgaaagcata ccgggaattg cccattgtct gggcattgaa
       61 gtttaggagg aaatatgaat tatgtgatga gttttgttat aggaggtctg atttgtacag
      121 ccggtcagat tataatcgat acattcaaga aaaacaatgc atatcttttg gtgctgctgg
      181 ttgtgacagg gtgtatactt ggatttttcg gagtttatga taagcttgtg gaaatagggc
      241 actcaggtgc aactgtcccg cttctcggat tcggtaattc attagccaag ggtgcaatgg
      301 aagaggctgc cggaaaagga ttcatgggag cactcagcgg gggagttata agaacagccc
      361 cgggagttgc agcagcactc atattcggtt acacagttgc agttatattc aatccccatg
      421 gagataaata gtttgtctta accccaattt atgccgccta gggagcaaat aaggtgattg
      481 aagaataaaa aagagttcag actaatttct gaactctttt acttgtcttt tcttatagcc
      541 atgaaccgca ggtcctattt ttaaaagatg agatcatttg cttaattttc tacggattca
      601 aggatttcat ctattggttt ttcggagtct tcttcagaat cattaaactc gtcaattata
      661 tcatcaataa tgtcttccag ggtatctccg tttccgttat ttccgttttc accgttcacg
      721 ccgccgttgt tttcttcatt cagccactgg tcatagtgcc acttggtgtg ataatagcag
      781 gttcttgtag ggatctgata ctgaaagtct tctgttaaga gtccggagaa ccctaatttt
      841 tcaacttcaa tctcgtttac gtgagtgatt tgaccatttt catcaactga catctgggtt
      901 ccatatattt gctgcagctc ttccaaggta aaaataattc tgtcctccaa aatttgctgg
      961 tataattttt tagcttcata atttgtttcc tttgcttccg ggtcataaag aggatctctt
     1021 accacaaaca ctctttcttc aaccaggttc ccggggcagt atatggattt gagcatatta
     1081 gagctggtgc atatttccgc cttaacatgc acgtcgcagg tttccgtagg ctgagttccc
     1141 ggtatgaagt attcggtaat aacccggctg cctctttggt cctgggcaca caggtctccg
     1201 ggaagcttac ctgattgtga acaaacactc ataggtacta aattcggatt cctggtaaat
     1261 gatgcaggag gcaggccctg atgtatcggg gtcataattc cgctccatag ccttgcattg
     1321 tttccgctgt caccggacag tttcatctgg acattatcgt ttccaaccca gattccaccc
     1381 acatagtatt gtgaaaagcc cacaaaccag aagtctccgt ttgcctgtgt agttccggtt
     1441 ttacctgcta cttctatacc taattcctga ggaagccttg ccctgtatga aagtcccgga
     1501 tttacagttg ttcttaagat atccttcatt aatgaggcta cttcagggct gactactata
     1561 tgtgtttcag gaatgttttc cagtattgtt tcgtccctgg agtttactac atgggtatac
     1621 acaataggct caacataaat gccatcgttg gcaattgcac cgtatgcggc agtcattccc
     1681 aggggtgaga atccttttgt aagtccacca agtgcaaggg atgccaaatt cacatcattg
     1741 tagtagggtc cttcctgagg agatacaaag gtatcatttt cagggttgtt ctggtcaatc
     1801 aggcccaatt ttgtcaatga gttaatagca gcatcaaatc ctatagtatc aagcatggtt
     1861 actgcatttg tgtttattga ctgctcgatt gattttctta gagtctgtat accccagtat
     1921 ttaatacttc tgtgttcata ccagttttta ggccatctgt cgccactttg gttgtatctt
     1981 gggaggtcat ctattgggta tgccgctgaa tatcccaaat ccaaagcagc caggtatacc
     2041 gacagcggtt ttattgttga gcccggctgt cttgcagcat ctgttgcacg attgaatgtt
     2101 ttgcttcctt caatctgcct tccgccgatc atacctttga ttttacctgt tctgtaatcc
     2161 attattacag ttgctgactg aggctgtaca atgccttttt cctggaagaa gaagtatcct
     2221 tccggtattc ttaatattcc gtcgcttcct atattaaaca ttcccggatt tttatccaaa
     2281 taagatttag gtattttaaa agatcctttt gaaccttttt tttcaatgac ttcataatta
     2341 tttcccacgt tgagagctcc tatggtgtga gaaacaaagt tcatcccgtc atcaatagta
     2401 taagcatcaa caatatcaat cgttgaggaa tatatgtcga attttttgga gtttattacc
     2461 agatttccgt tttcatcagt gctgaattca tcaggattca aataaatgct gttttcgtca
     2521 tccattatgt tttctctggc aaagaatata agctggccgt tttcattcaa aatattcaat
     2581 gctgaatcaa gcatcccatc tccttctcct ccggaccatc tgaaatatct ccagtcctgg
     2641 gcgatgggtt tatctcctga aggttctgca cccaaatata atacagaaaa attattatag
     2701 gactgctcaa gtgacttttg aatatttacg tcaatggttg aagtaattgt taaaccacct
     2761 ttgtacagat agctttcagc ttcctcatag gtcatgttta attcagccat taagtcttcc
     2821 acaacttttt cttttacata gtccatgggt gttgatgaaa taccttctat ttttgtctgc
     2881 cccggattta aagcggcacg catatcctct gccattgctg catcatattc ttcctgattg
     2941 atgtattcca gttcaagcat tctgtttaaa attgcatgct gcctgtcgat ggcattttga
     3001 ttgtagacgc atgcatattg aacggatcct ataaatacat aaccgacaat atcctcttca
     3061 ggaatttcat tgagactatc cagattgtat ctgttatagg gagcaaatga cggtcctttt
     3121 gcagctcctg ttaaaaggca gctttctgcc agtgtaaggt cgcttacatc ctttgaaaaa
     3181 tatgtgtatg ctgctgtctg tactccatag gcactttgac ctagggggat ggtgtttaaa
     3241 taattttcaa ggatttgttc tttggagaga actctttcta ccttgatggc taagtaaatt
     3301 tccttgatct ttctctcata ggatttttca tttgtgaggt acagattttt aaccagctgc
     3361 tgtgttatgg tgctggcacc ctgtgcagta gggtctcctt ctctcacnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnctt ttgacataaa ctacctccgc gtctgcatta
     3541 gttagtatac cacaatttaa gcaaatcttg ccaaagattt tatcacctca ctaattatat
     3601 aatagaaagg gcatgaaatc aatagtttat aaattgacga aaaaaatata taactccaaa
     3661 tggtttaagc ggaaggttct taaagaataa ttgcaaaaat acacctataa tgatatattg
     3721 tatatagacg ggcaataaat acaataatat tataattgta tgaacaaggc attgttagtg
     3781 tcatatacaa tgagcactaa attaaatttg cgggaatgaa taaagaattt gaaactgtgg
     3841 gaatctcata tattaaaggt atgtcaaacg gaggatttat ggaaaaggaa agatgtttac
     3901 tggttgcagt tcaggttgcc ggcgaagaag aaaatgatat ggaagaactg gcgcaattgg
     3961 ttgaaactgc agggggcgaa acggtgtcaa gcattgttca gaacaggcag agcattgaca
     4021 acagatttta tgcaggacag ggtaaaatag aagaaatagc aaattatgta aaagaacttg
     4081 atatagatac aatagttttc aatgatgaat taagcgggtc ccaaataagg aatattgaag
     4141 aaattgttga tgcaaaggtg atagacagga caaaccttat acttgacata tttgcaaaaa
     4201 gagccctaac caaggaaggg cagcttcaag tggaactggc acagttaaaa tacagccttc
     4261 caaggcttat aggtgttaat aaaaacctgt cccggttagg cggagggata ggcacaaggg
     4321 gaccggggga gcaaaagctg gaaatcgaca gaagaagaat aaaagaaaaa atcactgata
     4381 ttcaaaatca attggaagta ttggaaaagg taagagaaac caaaagaaag aaaagaatga
     4441 aggaccaaat ccccatagta tctattgtag gttataccaa cgcaggaaaa tctacattgc
     4501 tgaatgcatt gatggataat gaatacaggg aagaagggga aaacaaaaag gttttcgccc
     4561 atgatatgct ttttgcaacc cttgatactg agttgagaag agtgagactg cccggaggaa
     4621 gaagtgttgt tttttcagat actgtaggtt tcattaaaaa gcttcccaca caaattgtgg
     4681 aagctttcaa aggaacactg gaagaaatca aatatgccga ccttataatt caccttattg
     4741 acataaatga cgagaattta tcagcccaca aggaaacaac aattgagctt attgataaaa
     4801 tgacaggaat ggaaatacct gtactcactg tttataacaa ggtggataaa atcctggatg
     4861 aaaaaatatc tgttttaaac aacggcatta tttatatttc ggcaaagtat aattataata
     4921 taaatatgct tctggaagct gttgacaata taatcaacgg taaaaaaagc ttttatgatt
     4981 taaaaatacc aaacagtctc ttgaaaacct attattggct ttatgaaaac agaacaaccg
     5041 aaaatattga atttgaaggt gacggcgttt cacttagagc cttattgtac gaaaatgaaa
     5101 aggatgagta taaaaattat atgggagtaa ttgatgagta attataaatc catccacaaa
     5161 gaaggaagag atgaattaat tattaataaa tcaaggttca taggcactgc atgccctgtt
     5221 gaaacggaag atgaagcact ggagtttatt gacaaggtaa agagagaatt caaggatgca
     5281 acacataatg tttatgctta tgtaattgga gaaaattcaa acatacaaag atattccgat
     5341 gcaggagagc cctcagggac tgccggcatg cccgtattaa atgttataaa tcaggaaaat
     5401 attaaaaatg ctgcagttgt tgtaacaaga tatttcggcg gtgtgctgct gggagcggga
     5461 ggacttgtca gagcttatac aaaaggttca aaaattgctt tggaaagtgg aataatagta
     5521 gacaaaaaac tattttatga tgtatcattt aaaatagatt atacactatt aggaaaaatg
     5581 gataacgaac ttgttaagaa taattttatc cttgttgata gaatatatga ggaccgggtg
     5641 cttattaaac taatagcaga gaatgaaaat ttggataata tcggggcctt ggtcggtgaa
     5701 attacaagcg gcaaagctga aatatccgtg ggacaggcag catactattc cgtaaaaaac
     5761 gggaagatag tggaataagg aggagaaaat gtcaggacat tcaaaatgga gcaatataaa
     5821 aaatcgtaaa ggaaaacaag atgcattaaa aggtaaaata tttacaaagc ttgcacgggc
     5881 aataaccgtg gcggtgagag aagggggagc tgagccgggc tataattcaa cccttgcaac
     5941 agcaattgaa aaagcaaagg cacaaaatat gccaaatgac aatatagaca gagcaataaa
     6001 agcaggactt tcagcagctt ccggagaaaa cttcgaaaca atcacctatg aaggatatgg
     6061 tcccagcgga acagcgttta tggttcagtg cttaacagat aataaaaaca gaactgctgc
     6121 ggatgtaaga cattattttt ccaaatacaa cggaaatttg ggaacaacag gatgcgtagg
     6181 gtatctgttc gataaaaagg gagtaatttc tatcgaatca gaaggcgtta gcgaagatga
     6241 gctgatgatg gctgcccttg aaaccggtgc agaagacttt gaagctca
//
LOCUS       DAOPUT010000080         6244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_12461_length_6244_cov_21.458232, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000080 DAOPUT010000000
VERSION     DAOPUT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12461_length_6244_cov_21.458232"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..386)
                     /locus_tag="PKK75_06790"
     tRNA            complement(297..386)
                     /locus_tag="PKK75_06790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(350..352),aa:Ser,seq:gga)
     gene            complement(584..1024)
                     /locus_tag="PKK75_06795"
     CDS             complement(584..1024)
                     /locus_tag="PKK75_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HNZ83343.1"
                     /translation="MREFKGRVVVPGRAKAPALVSRQGFNTLASFQKSLMSKEKEAIC
                     SDQNNSDVYKKKMTSKALCMPQTIGSTTGGLVLYCASTFNVAPSCLLFSKTIDSLAAA
                     GAIMSDIWTDNNLPTIDNLGDEFLEYVKDDMTIEILDGGVVRVS"
     gene            complement(1026..2300)
                     /locus_tag="PKK75_06800"
     CDS             complement(1026..2300)
                     /locus_tag="PKK75_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HNZ83344.1"
                     /translation="MHLTEDEKDILNGSQGETMAKIMKTLVMYGDTFGADSLVDVTSD
                     AGHLVTSFGISLMSPVYDLMDELLNAGIVSKQPFTVDPRPVDLENVPSNIIQKLVFRF
                     MYGKQDYYEDQLKKMGLKSKDGFSCTAYMEQVGNIPNKGDILSWAESSAVVYANSVLG
                     ARCNRNSGIIEMFGSILGKVPNFGLLTDKGRKASWIIEVKTGKLPEAQVLGSAIGMKV
                     MEDVPYIKGLDKFLGNELDGWVKDYLKDMGAATASNGAVGLYHVEGLTPEAAESGEDL
                     IAQNPSVYVIDDAELQRVKDSYPVIWKDPDARPKLCFIGCPHLSLRQLKDWTNRIEEG
                     LKKAGKTKVLIPTVLTAASPVLKEFNELPEAKVLKRLGVSTSYICPLMFMNNPTCGKM
                     PVITNSNKLRTYTTSRYYTDDEIVSVITEGRQ"
     gene            complement(2330..4591)
                     /locus_tag="PKK75_06805"
     CDS             complement(2330..4591)
                     /locus_tag="PKK75_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ83345.1"
                     /translation="MKTDKIINELVGNYGKWFYDDEVLKSGKMTQNLYPYKSIFSPVE
                     INKITIKNRLVMAPMGNISMCDETGRPNDRMLQYFFERAKGGVGLITTGLVPICHGLD
                     PSVTEPGQLSYFPRIDRSRTVYAGWRDLSAGVHSYGAKIFIQLTPGLGRVGNPQCLLN
                     FKKFPKSASMNPNFYMPQIPSTRLSDRKAKKIIKNAGQASADAKAFNIDGVYLHGHEG
                     YLLDQMTNTAFNRRKMGRYADWQAFGLDMIREIRKRVGPNYPIMYRIDLSLALNETYG
                     EKMDNKSLRKFKNGRTVEMTLKYMANLVKEGVDIFDVDLGCYDNWWLPHPPASMPPGC
                     FLDISKIAKDYFKENNILSNAGKEVPVVAVGKLGYPDLAEQALRDEKCDMVMLGRPLL
                     ADPDWPNKVYSGRVNEIIPCIGCHEGCINEFVEGGHPQCAVNPRTSFEYIMPHNLIPA
                     KEKKKIGVIGAGPAGVNMAITAAKRGHDVTLVEESGRVGGKTIPGSVPKIKYEIANYR
                     RYLEDQVIKAQEAENFTYLPNTTATIDWLKDQKFDSIVFAMGTKDSNLPFKGLDKVNS
                     IEATDLLLHPEKIGEAKKAVVVGGGVVGCETAYWLSYEHNLDVKVVEMEKYFMNHTCT
                     ANRGHLLHYMEKAGVELLNCTRVTGFEENAVKVMQNISESVPDPYITWHPVLPENVVN
                     PLEPKFEVKEEEKILNADLVVIATGGKPKDHLFYEVQKENIAPELYNIGDSFSGGKVL
                     EATRAANALGRRI"
     gene            complement(4831..5691)
                     /locus_tag="PKK75_06810"
     CDS             complement(4831..5691)
                     /locus_tag="PKK75_06810"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNZ83346.1"
                     /translation="MEREIGIIGIGHVGSMLVQKFTEFNLFAEENIYAANRTVEKLQS
                     LKEKYPSINICTSVGELAKKCSNIILCVKPLDLPYVLLEIQPYIDEKTYLMVSTSMIK
                     HEDIRKFHTGSITIFMPTLISMVNGGVSLVYHNEHVDEKKKEYFHKILNPFSEIKVLG
                     EEDINLSQNMTATFPGLYAEIMLEFVKAASKRSKNVSDEDLEHMLLVSLLGASRLLLE
                     KNMSFEETIKRVSTKGGTTNAGVTVLKKMLPEVFDATLDASITRHDEMSNAVSNNIEE
                     LVSFKNREAI"
     misc_binding    complement(5736..5932)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1706 a         1396 c         1035 g         2107 t
ORIGIN      
        1 tttatcaatt tatttttgga caaaataaaa gatattatat atcagaatct ctgttaaatt
       61 tgtcataatt tgtcaaaaca tatctttatt tgttgtcttt caaaactgct ataataaact
      121 gtagaaattt ttaataaaaa tcccgatcat attattaagc tgatagattt gtttatacct
      181 taataaaata accctatcca taaaaagata gggtattatt tataatatgg cgaacttttg
      241 gcgaacttca taaaatatcg actaatccat aagctataca tatttgattt caattttggc
      301 ggagagcaag ggattcgaac cctcgataca gtttcccgta cacacgcttt ccaggcgtgc
      361 tccttcaacc actcggacaa ctctccgtaa tgctttacag caaagtaaat tataactgaa
      421 aacagcggta gttgtcaatg gtattttcag ttatcgcaaa attatggtgg aattcacata
      481 ttatttcttt aatacaatag tccaacgcta agttttatta ccttaattgt gtaatatcac
      541 ttagcgttgt attccacccg aatatttaat tttctgctaa taatcagctg actctcacta
      601 ccccgccgtc cagtatttca atagtcatat catcttttac atattccagg aattcatccc
      661 ccagattatc tatggttggc aggttattat ccgtccagat atctgacata attgcacctg
      721 cagctgccaa ggagtcaatt gtttttgaga aaagcaagca ggacggagcc acattaaagg
      781 tacttgcgca gtataatacc agaccgcctg ttgtggaacc tattgtttgt ggcatacaaa
      841 gtgccttact tgtcatcttc tttttatata catcactgtt attctggtct gaacatatgg
      901 cttctttttc cttggacata agactttttt ggaaacttgc caatgtatta aacccttgac
      961 gggaaacaag agccggcgcc ttagcccttc ccggaacaac tactcttcct ttaaactctc
     1021 tcattttatt gcctcccttc ggtaataaca ctcacaatct catcatctgt ataatagcga
     1081 gaggtagtat aggttctcag tttatttgaa tttgtaataa cgggcatctt gccgcaagtt
     1141 ggattgttca taaacattag aggacaaata tatgaagtgc taacccctaa tctttttaaa
     1201 acctttgcct caggtaattc attaaactcc ttcagcacag gagatgcggc tgttaaaact
     1261 gtcggtataa gcactttggt tttacctgct ttttttaacc cctcttcgat gcgatttgtc
     1321 caatccttca gctgtcttaa tgacagatga ggacacccga taaagcacag tttaggtctg
     1381 gcatcaggat ccttccatat aacaggataa gaatctttaa ccctttgtaa ttctgcatca
     1441 tcaataacat atacactggg attttgtgca atcaaatctt ctcctgactc ggctgcttca
     1501 ggagtcaggc cttcaacatg gtaaagtccc acagcaccat tacttgctgt agctgcgccc
     1561 atatccttta aatagtcttt cacccaacca tccaactcat ttcccaaaaa tttatccaac
     1621 ccttttatat agggcacatc ttccataact ttcatcccta tggcactgcc taaaacttga
     1681 gcttcaggca attttccggt cttaacttct ataatccagg aagcctttct gcccttatca
     1741 gtcaatagac caaaattagg aaccttgcct aaaatactgc caaacatttc gatgatcccg
     1801 gagtttctgt tacagcgggc tcctaaaaca gaattagcat aaactactgc tgaactctct
     1861 gcccagctga gtatatcgcc cttatttggt atattcccca cttgttccat ataggctgta
     1921 caggaaaatc catcctttga ttttaatccc atctttttta attggtcctc ataataatcc
     1981 tgtttgccgt acataaatct aaaaacaagc ttttgaataa tattagatgg tacattttct
     2041 aagtcaacag gtcttggatc aactgtaaaa ggctgcttgg aaacaattcc agcattgagt
     2101 agttcatcca tcaggtcata gactggactc ataagagata tgccaaaact ggtaactaaa
     2161 tggcctgcat cactagtcac atcaactaag gaatctgctc caaaggtatc accatacata
     2221 accagggttt tcatgatttt agccatggtc tctccttgac tgccatttaa gatatctttt
     2281 tcatcttcgg taagatgcat cttatagtta tacattttat tccctccttt tatatacggc
     2341 gtcccagtgc attagctgcc cgggtagcct ccaatacctt gccgccgcta aagctgtctc
     2401 cgatattata aagctcggga gcaatatttt ccttctgtac ctcgtagaat aaatgatcct
     2461 ttggttttcc tccggtggca ataactacta agtctgcatt taaaatcttt tcctcttcct
     2521 tcacctcaaa cttcggctcc agcgggttta ctacattctc cggcagtacc ggatgccatg
     2581 ttatataagg atcaggaacg ctttcagata tgttttgcat aacttttact gcgttttctt
     2641 caaatccggt tacccgagtg caatttaata attctacacc tgctttttcc atatagtgga
     2701 gtaaatgtcc ccggttggca gtacaggtat ggttcataaa atatttctcc atttcaacta
     2761 cctttacatc cagattgtgc tcataactta accaataggc tgtttcacat cccacaacac
     2821 cgccgccgac aacaacagcc ttttttgcct ctccgatttt ttcaggatgt aacagcaagt
     2881 ctgttgcttc aatagaattc accttatcca aacccttaaa ggggagattg gagtctttcg
     2941 ttcccattgc aaatactatg gaatcaaact tttgatcttt tagccaatca attgttgctg
     3001 ttgtgtttgg aagataggta aagttctctg cctcttgggc ctttataacc tggtcttcca
     3061 aatatcttct gtaattagca atctcatact tgatctttgg aacacttccc ggtattgttt
     3121 tcccgccaac cctgccgctc tcttctacca aggttacgtc atggcctctt tttgcagcag
     3181 taattgccat attgacaccg gccggacctg ctcctataac ccctattttc tttttttctt
     3241 tagctgggat caggttatgg ggcataatat attcaaaact tgtccttgga ttaacggcac
     3301 attgtgggtg tcccccctcg acaaactcat taatacaccc ctcatgacat ccaatacatg
     3361 gaattatttc attcactctt ccagaataga ctttattggg ccaatccggg tctgccagta
     3421 aaggacgtcc taacattacc atgtcacatt tctcatcccg aagtgcttgt tcggctaaat
     3481 caggatagcc tagctttcct acagctacta caggaacttc ttttcccgca ttggacaaaa
     3541 tattgttttc tttaaagtag tcttttgcaa ttttggaaat atccaggaag catccagggg
     3601 gcatgctggc cggcgggtgg ggcagccacc aattgtcata acagccaagg tctacatcaa
     3661 agatgtcaac accttccttt acaaggtttg ccatatattt tagggtcatt tcaactgttc
     3721 tcccattctt aaacttcctc agggatttat tgtccatctt ttctccatag gtctcattga
     3781 gagctaagga tagatctatt cgatacatta tcgggtaatt ggggcccaca cgttttctta
     3841 tttctcttat catatccaac ccgaaggcct gccaatcggc atatcttccc atctttctcc
     3901 ggttaaaggc agtgttggtc atctggtcca ataagtagcc ctcatggcca tgcaagtaaa
     3961 ctccgtcgat attaaaagct ttggcatccg ctgaagcttg tccggcattt tttataattt
     4021 ttttggcttt tctatcactt aagcgggtac ttgggatttg aggcatataa aaattaggat
     4081 tcatagatgc tgactttgga aactttttga agttaagaag gcattgtgga tttcctacac
     4141 ggccaagacc cggtgtcaat tgaataaaaa tttttgctcc atagctatga actcctgctg
     4201 acaaatcacg ccagccggca tatactgtcc gggaccggtc tatcctgggg aaatagctaa
     4261 gttgacccgg ctctgttact gatggatcca gcccatggca tataggcact aatccggtgg
     4321 taatcagccc aactccgcct ttagctctct cgaaaaagta ttgcagcatt cggtcatttg
     4381 ggcggcctgt ttcgtcacac atagagatat tacccatagg tgccataaca agtcgattct
     4441 taatagtaat cttattaatt tcaacgggtg aaaatattga cttgtaagga tataaatttt
     4501 gtgtcatttt acctgatttt aatacttcat cgtcataaaa ccattttcca taattaccta
     4561 ctaattcatt aataattttg tcggtcttca ttctttcctc cttttgtttt tcagatcaat
     4621 gtcttttacc aaacaatgct ctgttactta ttgtaattat aacatacaaa tttttcctgt
     4681 tcaatccatt tcaatatgaa tactgaaagg acttcttata aaagtgttta ttacaaggca
     4741 ggcaagttgc aggaaaagct ttggggacat tcctcctgct aacagcgctt tccctaaaca
     4801 tggtggtgtg tccccataat cttgtgattt ttatatagct tctctatttt taaagcttac
     4861 taattcttca atgttgtttg atacagcgtt actcatttca tcatgtcttg ttatggatgc
     4921 atcaagtgtt gcatcaaaaa cctccgggag catttttttc agtacagtta ctcccgcatt
     4981 agttgtgcct ccttttgtcg acacccgttt tattgtttct tcaaagctca tgtttttttc
     5041 caataaaagt cttgaagcgc caagtaaaga aacaagcagc atgtgttcca gatcttcatc
     5101 tgatacattt ttgctgcgtt ttgatgcagc tttcacgaat tcaagcatta tttcagcata
     5161 gaggccggga aaggtagcag tcatgttctg gcttagattg atgtcttctt ctcctaaaac
     5221 ctttatttca ctgaaaggat tcaatatttt atggaaatat tcttttttct tctcatctac
     5281 atgctcgtta tgatatacaa gtgaaacgcc tccatttacc atggatatta atgtgggcat
     5341 aaatatggta atgctccctg tgtgaaattt tctgatatct tcatgtttaa tcatggatgt
     5401 tgataccatt aaatatgttt tttcatcaat gtagggctga atctccaaca agacatatgg
     5461 cagatccaaa ggcttaacac acaaaattat attgctgcat ttttttgcca gctcccctac
     5521 gcttgtgcag atgtttattg agggatattt ttctttcaag ctttgaagtt tttcaactgt
     5581 gcggttggct gcatatatgt tttcttcagc aaacagatta aattctgtaa atttttgcac
     5641 aagcattgac cctacgtgtc caattcctat tattcctatt tccctttcca ttttgacctc
     5701 caaatatttt ttatataaaa ataaatccac cccaatatag aggcggatat ccacggtacc
     5761 actctagtta ctgcatatgc agcatctcat ttcaaataac ggctttcccg tttgggttgt
     5821 tactcccaaa tgctccaaga cgggttcaat aacaagaggg acgaaaactt cacaccaact
     5881 gttttctctc tttagtcctt tattatttac tgcttctttt ccctgcaata tatttaatta
     5941 ttatacaatc aacaataata aaacccgcct caaaacagag gcgggatatc cgcggtacca
     6001 ctctaattgc tgtttccagc atctcgtttc atataacggt gtcatccgtt tgggttgtta
     6061 ctcccaaaag ctccgaggcg ggttcaacat gcagcgggac aaaaaccttt caccctcggt
     6121 tccctctctt ttgaccatgc atatttactg gttctcttct aagcaagatt aatttaaatg
     6181 caatgatata ttatatgttg atttttgtca agagtttcta aaatatttat tcaaagaatt
     6241 ttaa
//
LOCUS       DAOPUT010000081         6221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_12519_length_6221_cov_17.699319, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000081 DAOPUT010000000
VERSION     DAOPUT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12519_length_6221_cov_17.699319"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKK75_06815"
     CDS             <1..204
                     /locus_tag="PKK75_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HNZ83347.1"
                     /translation="KLVVPEAQQAINELKMEISHELGMPYIQGTSSNMYPAIVNGLSV
                     REMVKRGEQMLVDKDAPTDKDVY"
     gene            complement(260..1156)
                     /locus_tag="PKK75_06820"
     CDS             complement(260..1156)
                     /locus_tag="PKK75_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNZ83348.1"
                     /translation="MKHFFKVFFISLFCFSLIIIGGLFCLKGGLTDRNADASDIFKIL
                     SKDKDKRINVLVLGLEDTRSDTIMIASYDTKNKTGDVVSIPRDTYVDRDGFVNSGNNK
                     INSVYGVKGIEGLSEIIYNITGIKIDRYVTIDYDGVRDAVDAVGGVEVDVPFHMRYTD
                     PYADPPLDIDIPQGKQLIKGDRAMEFLRFRKTNYAGYSGYSDGDLGRIQAQQEFVKAV
                     IKKALSIKLPLVIKEVYPYVNTDFTLAEATSLAVGSMGLSAENINFHTLPGYTQTRNG
                     LSFYILDYLAAKDLVYSILNTN"
     gene            complement(1258..1806)
                     /locus_tag="PKK75_06825"
     CDS             complement(1258..1806)
                     /locus_tag="PKK75_06825"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNZ83349.1"
                     /translation="MKIYTKTGDKGQTSLYDGTRIDKDSLRVESYGTVDELNSYIGFA
                     MKFVDDDRIKDSLKRIQMRLFFVAGELATLEEGRYIYRVREEDIAALEKIIDDYLPKI
                     SGVDKFIVPGSSEASAALHVARTICRRAERRIISLKNKEEVSDTLLKFVNRLSDVLYT
                     YARLLESDLAIMDFEKAYNEKL"
     gene            complement(1803..2681)
                     /gene="rsmA"
                     /locus_tag="PKK75_06830"
     CDS             complement(1803..2681)
                     /gene="rsmA"
                     /locus_tag="PKK75_06830"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNZ83350.1"
                     /translation="MDEIKLYSPKVIMDILDRYGFKFSKSLGQNFLIDGNIIKRIAEI
                     ADLDENSGIIEIGPGFGTLTQVLCRKAKKVLAIEVDKSLKEVHRGTLNYPNLKIIYED
                     FLKVDVNKLIEEEFQGLDVKIAANLPYYITTPIIMKILEEKYNISKIVVMVQKEVAQR
                     LSAKAGTGDYGAITLAVRYRADTDIKMIVPNTVFMPKPKVDSAVVEFKILSEPRIKVM
                     DEEMLFAVIKASFGQRRKTILNGLSNNLNMSKEIIKDSLVCAGIDPGIRGEKLALEEF
                     GRISDEISKRKSGGFQ"
     gene            complement(2674..3222)
                     /gene="rnmV"
                     /locus_tag="PKK75_06835"
     CDS             complement(2674..3222)
                     /gene="rnmV"
                     /locus_tag="PKK75_06835"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HNZ83351.1"
                     /translation="MIKEIIVVEGKADISAVKQAVDAQVISTNGLGINEEIINIIKRA
                     SKNRGIIILTDPDYPGKKIRNILASQIENCKHAFIPRDKADKAGDIGVENASKEAIIE
                     ALKNARAEVAENRQEFSKSDIVCYGLGGNDYSAKRRSAIGDELGIGYCSAKQFLKRLN
                     SFGITREELEDAINKTIEGFNG"
     gene            complement(3215..3874)
                     /gene="udk"
                     /locus_tag="PKK75_06840"
     CDS             complement(3215..3874)
                     /gene="udk"
                     /locus_tag="PKK75_06840"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNZ83352.1"
                     /translation="MKKPIFIGVSGGTGSGKTTIVNRICSEVPEKSVAIMEHDSYYKD
                     QSPMTYEQRCKTNYDHPFAFDTDLFVNHIKELKKGNSIEKPIYDYGIHNRKKETIIVE
                     PKEIIIVEGLLVFYEERIRELLDIKIYVDTDADIRILRRIIRDINERKRSLDSVINQY
                     MNTVRPAHEQFIEPSKKYADIIVTEGGNNLVAIDLMVTKIKSLLNMSRSETIKQGADI
                     D"
     gene            complement(4072..>4449)
                     /locus_tag="PKK75_06845"
     CDS             complement(4072..>4449)
                     /locus_tag="PKK75_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNZ83353.1"
                     /translation="EEHEIIIEQAEISRGGFRFKSEMDMAATAYDLSYESCGKYPDHP
                     EYGMTASGTKAQPGTVAVDPDVIPLGTKLYIASTDGSPDYGFATALDTGSAIKGYRID
                     LFMEDNKTAMNYGVRQVKVYILE"
     assembly_gap    4450..4549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4675..5544)
                     /locus_tag="PKK75_06850"
     CDS             complement(4675..5544)
                     /locus_tag="PKK75_06850"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF005491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HNZ83354.1"
                     /translation="MKEIPKIMAVQDMSGVGRCSLTVIIPVMSALGCQVCPLPTALLS
                     NHSEYKDFYFFDFTAHMEEYYSFWEKNNFWFDCIYSGFIGSEDQIDIILDIIGKVKKK
                     NNKALIVVDPVMGDHGFTYATYNGEMITKMSHLVEKADIITPNLTETSILLGKKYSSD
                     KVTLPQVKEYLKELCSLGPEISLITGIMTEEGRHVNVCYNSNDDKFWLAPFNYVNKRY
                     PGTGDLFTSLFLGYYLKGKSLPEAIEEAARFVSLSVLTSYEAGVPSSEGVIFEKIMKE
                     LYNEIEEYKYTRI"
     gene            complement(6025..>6221)
                     /locus_tag="PKK75_06855"
     CDS             complement(6025..>6221)
                     /locus_tag="PKK75_06855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNZ83355.1"
                     /translation="VDSYVPYAGKLDDGLGSTLHKIKSTMCNCGTRTIKELQKRGKLT
                     VVSATSLTEGGAHDVILKNQ"
BASE COUNT         1676 a         1310 c          976 g         2159 t
ORIGIN      
        1 aaattagtag ttcccgaagc acagcaggct ataaatgaat taaagatgga aatatcccat
       61 gaattaggga tgccatatat acaaggcacg tcatccaata tgtatcctgc tattgttaac
      121 ggcctcagcg tcagggagat ggttaaaaga ggcgagcaaa tgttagtaga taaagatgct
      181 cccactgaca aggatgttta ttgacaggtc atacaaaaac gcctcaaatg aggcgttttt
      241 caattttcat aaaatagggt cagtttgtgt ttaatattga ataaaccagg tcttttgctg
      301 ccaaataatc taatatataa aatgacagtc cgtttctggt ctgggtgtat ccggggagag
      361 tatgaaaatt aatgttttca gctgacagcc ccatgcttcc cacggccaga gatgttgctt
      421 cagccaaggt aaagtcagta ttgacatatg ggtatacttc ttttatcacc aaaggcagtt
      481 taatagacaa tgccttttta ataaccgctt tcacaaattc ctgctgggct tgtatgcggc
      541 ctaaatcacc atctgaatat ccggaataac ctgcataatt ggtctttctg aaccttagaa
      601 attccatggc cctgtcccct tttatcagtt gttttccctg gggtatgtct atatcaagcg
      661 gggggtcagc atatgggtct gtatatctca tgtgaaatgg cacatccacc tctactccgc
      721 ccacggcatc cacagcatct cttacaccgt cgtaatcaat ggtaacatac ctgtcaattt
      781 taatgcccgt aatattataa ataatttcag ataatccttc aatgcccttg actccataca
      841 ccgagtttat tttattgttg ccgctgttta caaatccgtc cctgtccaca tatgtatctc
      901 tgggtatgga aacaacatcc cctgttttat tcttagtatc atagctggct atcataatag
      961 tatcggacct ggtatcttcc agtcccagca caagcacatt tattctcttg tccttatcct
     1021 tgcttaatat tttaaatata tcagaagcat cggcatttct gtctgttaag ccgcctttaa
     1081 ggcaaaacaa tcctcctata attatcaaag aaaaacaaaa aagcgaaata aaaaaaacct
     1141 tgaaaaagtg tttcattaaa attcctccaa acttatgtgt caaagcttat attatcacaa
     1201 aatattcatt tgtaaagaat tttaatctta atttttgttt tagaattcca actggcatca
     1261 tagtttttca ttgtaagctt tctcaaaatc cattattgcc aggtcgcttt ccaaaagtct
     1321 tgcgtatgtg taaagtacat ccgacagcct gtttacaaac ttcaaaagcg tatcgcttac
     1381 ttcttccttg ttttttaagc ttataattct tctttcagct ctgcggcata ttgttcttgc
     1441 gacatgaagg gcggcagatg cctctgaaga gcccggaaca ataaatttat cgacaccgct
     1501 tatttttggc agataatcgt caattatttt ttccaatgca gcaatatctt cttctctgac
     1561 cctgtagata taccttccct cttcaagagt tgccagctct cctgctacaa aaaataaacg
     1621 catttggatt cttttaaggc tgtcctttat cctgtcatca tcaacaaatt tcatagcaaa
     1681 acctatgtaa gaatttaact catcaacagt tccataggat tccactctta atgaatcctt
     1741 gtcaatcctg gttccgtcat ataaagacgt ctgtcctttg tcacctgtct tggtataaat
     1801 tttcattgaa atcctcctga ttttcttttg ctgatttcat cagaaattct tccgaattcc
     1861 tccaaagcca gcttctctcc tcttataccc ggatctatgc ctgcgcaaac caatgaatct
     1921 tttattattt cctttgacat attcaaatta ttgcttaatc cgtttaaaat tgtttttcgt
     1981 ctctgcccga aggatgcttt gattactgca aaaagcattt cttcatccat tactttaatt
     2041 ctcggttcgg aaagaatttt aaactccaca actgcagaat caacttttgg tttgggcata
     2101 aatacggtat ttggaactat catttttatg tctgtgtccg ctctgtaccg gacggcaagg
     2161 gttatcgcac cgtaatcccc ggtgccggcc tttgcactaa gtctttgagc cacttccttt
     2221 tgtaccatta caacgatctt ggaaatatta tatttttcct ccaatatttt cattatgatt
     2281 ggagttgtta tgtagtaggg aagatttgca gctattttca catcaaggcc ttgaaactct
     2341 tcctctatga gcttatttac atccaccttc aaaaaatcct cataaatgat tttaaggttg
     2401 ggataattaa gcgtccccct gtgaacctcc ttcaaactct tatcaacttc tatggcaaga
     2461 accttctttg ccttcctgca caagacctgt gtcagggtgc cgaagcccgg tcctatttct
     2521 atgatacctg aattttcatc caaatcagca atttccgcaa ttctttttat tatattcccg
     2581 tcaatgagaa aattttgtcc caggcttttg ctgaatttaa agccatacct gtcgagtata
     2641 tccataatta cctttggtga atacagtttt atttcatcca ttaaaaccct ctattgtttt
     2701 attaattgca tcttccaatt cttctcttgt tatgccgaag gagttaagcc tctttaaaaa
     2761 ttgctttgcg ctgcagtatc ctatacctaa ttcgtcgcct attgcacttc ttcttttggc
     2821 tgaataatca tttcccccta agccgtagca aacaatgtcg ctcttggaaa actcctgtct
     2881 gttctctgca acctctgccc tggcattttt tagtgcttca attattgctt cctttgatgc
     2941 attttcaact cctatgtccc cggctttatc cgccttgtcc cttggtatga atgcatgctt
     3001 gcagttctct atttgagaag caagaatatt tctaattttc ttccccgggt agtccgggtc
     3061 ggtcaatatt attattcccc tgttttttga tgctctttta ataatattaa ttatttcttc
     3121 atttataccc aatccgttag ttgatatgac ctgggcgtca acagcctgtt tcaccgctga
     3181 aatatccgcc ttcccttcaa ccacaataat ttccttaatc aatatctgct ccttgtttga
     3241 ttgtttccga tctgctcata ttaagcaagg atttgatttt agtaaccata aggtcaattg
     3301 ccaccaaatt gttgccccct tctgtaacaa taatatccgc atattttttg cttggttcta
     3361 taaactgttc atgggcaggt cttaccgtat tcatatattg gttaattaca gaatcaagac
     3421 ttctttttct ctcattgatg tcccttatta ttcttcttaa gattcttatg tctgcatccg
     3481 tatcaacata aatcttaata tccaaaagtt cccttattct ttcttcataa aataccagaa
     3541 gtccttccac aataataatt tccttgggct caacaataat tgtctccttt ttcctgttat
     3601 gtattccata gtcataaatt ggcttttcga ttgaatttcc ttttttcagt tccttgatat
     3661 ggttaacgaa caagtctgta tcaaatgcaa agggatggtc gtaatttgtt ttgcatcttt
     3721 gttcataggt cattggactt tggtccttat aataggaatc gtgttccata attgctacgc
     3781 ttttttcagg aacctcggag caaatcctgt ttactattgt tgttttgcct gaacctgttc
     3841 ctcctgatac tcctataaaa atcggttttt tcataatatc ctctccaaaa gtattattaa
     3901 taacaatata caacatttta ctcgatttta ccatacaaaa atgctaaggg aaaaatgaaa
     3961 aattgctgtt gccagcaatt ctcctgtctt cggggacatt cctctattgg ctatcctgca
     4021 aagaatatct tactatagag gtgtgtcccc ataccttaaa acttattgaa gctactccaa
     4081 aatataaact tttacctgtc ttacgccata attcattgcc gtcttgttgt cttccataaa
     4141 caagtcaatt ctgtaccctt taattgccga gccggtgtca agggctgttg cgaagccata
     4201 atcaggactg ccgtcggtag aagctatata tagcttcgtt ccaagaggta ttacatctgg
     4261 gtctaccgca actgttcccg gctgagcttt tgtgccggat gccgtcatac cgtactcagg
     4321 atggtcagga tattttccgc agctttcata agataaatca taagctgtgg ctgccatatc
     4381 catttcactt ttgaatctga aacctcctct tgagatttcg gcttgttcaa taattatttc
     4441 atgttcttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gcaaagagtt
     4561 tatatacttc acattttaga aggtttgtaa taataaggca atttttacta aatgttttcc
     4621 aaaattgaca tataaaaaac agcctgaaaa agactgtttt tatcgcattt atatttatat
     4681 gcgcgtatat ttatattctt ctatttcgtt gtacaattct ttcataatct tttcgaaaat
     4741 tacaccttcc gagctgggca cccctgcttc atagcttgta agaaccgata gcgatacaaa
     4801 tcttgctgct tcctctattg cttcaggaag gctcttccct ttcaggtagt aaccaaggaa
     4861 caaagaagta aataagtccc ctgtacccgg atatcttttg tttacatagt taaagggtgc
     4921 cagccaaaac ttgtcatcat tgctgttata gcatacattc acatgtctgc cttcttctgt
     4981 cataattccg gttatcagag aaatttcagg ccccaggctg caaagctctt ttaaatactc
     5041 ttttacctgg ggaagagtaa ctttatcact ggaatatttt ttcccaagca ggatgctggt
     5101 ttcagttaaa ttcggtgtaa taatatctgc tttttctact aaatgtgaca tttttgtaat
     5161 catttcgcca ttataagtgg cataggtaaa accgtggtcc cccattacag gatctaccac
     5221 aatcaacgct ttattgtttt tcttcttaac cttgccgatt atgtcaagaa taatgtctat
     5281 ttggtcttcg gagcctataa atccgctgta tatacaatca aaccagaaat tatttttttc
     5341 ccagaaagaa tagtattcct ccatgtgagc agtaaaatca aaaaagtaaa agtccttgta
     5401 ttcagaatga ttgcttaata atgccgtagg caatgggcat acctggcatc ccaaggctga
     5461 cataacagga ataataactg tcagtgagca tctccccact cctgacatat cttgaactgc
     5521 cataattttt ggaatttctt tcatattgta tcctttcgat aaaagtatgt tgtttcgctt
     5581 atcttacagt attcggggac attcctcctg cagccggata ctgtctatgc cggtgtgtac
     5641 accatgttct gcggttttgt tcccatacct attttaaatg tattctatca ctttctaaag
     5701 gtacagtttt gctaaatttg aagggtacag caatacgatt aaaaaaatga tttcttttat
     5761 agaaatcatt tttttagggt aaagatttat taaataactc tttacaaggg gggctagtta
     5821 attacatcat atgtattcga gcctgtaaaa ttatttttgg ggttgctatg aataatttct
     5881 attgttaact tttagtgccc ttacaaaatt tttacatctc ttacatctaa tcacagtata
     5941 acaaatctcc tatatttatg caataaaaat atttcaattt taatttgtga atttattaaa
     6001 tttcattaat acttacaatt ggggctattg attcttcaaa ataacatcat gggctccgcc
     6061 ttcagttaaa gatgtagctg aaaccaccgt gagctttcct cttttctgga gctctttgat
     6121 tgtccttgtg ccgcagttgc acatggttga ttttatttta tgaagggtgc ttccaagtcc
     6181 gtcatcaagt tttcctgcat aaggcacata tgaatcaacg c
//
LOCUS       DAOPUT010000082         5894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_13461_length_5894_cov_19.372324, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000082 DAOPUT010000000
VERSION     DAOPUT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13461_length_5894_cov_19.372324"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..529
                     /locus_tag="PKK75_06860"
     CDS             113..529
                     /locus_tag="PKK75_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83356.1"
                     /translation="MEHKITAGLSAIHKREGTVRRTYTYSGSNDSLDYLLTTPEVLDN
                     IIEAAAKMLDPLLPNGYITVGKYIELSHEQPTLTLTGHAISTILTVTEITGNKVFLDF
                     SCHDEIGLICKGKYERAIVNKEKLMESTYRRAQSKM"
     gene            671..799
                     /locus_tag="PKK75_06865"
     CDS             671..799
                     /locus_tag="PKK75_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83357.1"
                     /translation="MKDVSFAFSVYLLGFAVSVSIAGLIKLILFIIRKLSRKEANI"
     gene            796..2004
                     /locus_tag="PKK75_06870"
     CDS             796..2004
                     /locus_tag="PKK75_06870"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ83358.1"
                     /translation="MNFISLFQGVGTLFVQEPKIAAARIVLILLGILLVYLGKKEILE
                     PLIMIPMGFGMAAVNSGVLFLSAAQIGTIFMDPMVTNTEELMTILQIDWLQPIYTFMF
                     SNGLIACLVFMGIGVISDVGSVLKYPFTGMLIAMFAELGTVITFPIAHSMGFTYGEAA
                     AISLIGGADGPMVLFASLALAQDLFVPITVVAYLYLSLTYGGYPFLVRLLIPKDIRAI
                     VIKTSKTKRNNITSNEKLAFDIFACIVLCLLFPSASPLFLSFFLGNAIRESGITRYAK
                     LLEDVFLYSATFLLGLTLGVLCEASTILNPKILPLLVLGIIALLISGIGGIIGGYLVY
                     FISGKNFNPVVGIAGVSCVPTTAKVAQKEVNRVTKYSFILEYAMSANICGVITTAILT
                     GIYVTLVPLL"
     gene            2045..2596
                     /locus_tag="PKK75_06875"
     CDS             2045..2596
                     /locus_tag="PKK75_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNZ83359.1"
                     /translation="MIVEFEGIKPTIDKKTYIADGSHIIGRATIKEYSSIWFNTVVRA
                     DINCIEIGRYVNIQDNSVIHVSNDYPAIIGDYVTIGHNATVHACKIEDHCLIGMGSII
                     MDGAVIGRGSIVAAGTVVKKGQHIPPFSVAAGVPAKIIRKTAEEDISKIHSQALKYKD
                     VWSIRYGIMPDAGGESYDYTDII"
     gene            2715..3851
                     /locus_tag="PKK75_06880"
     CDS             2715..3851
                     /locus_tag="PKK75_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ83360.1"
                     /translation="MDFLIEGIMAVTWKQVVMWIIGGLLIYLAISKELEPSLLLPMGF
                     GAILVNIPMSGVLNQTLDGIGHVQGIIDWLFEVGINASEAMPLLLFIGIGAMIDFGPL
                     LSNPRLLLFGAAAQFGIFATITAATLMGYGLADAASFGIIGAADGPTSILVSQVLESN
                     YIGPIAVAAYSYMALVPIIQPFAIKLVTTKNERKIRMSYNPKSVSRMTRLLFPITVTI
                     IAGLVAPASVSLVGFLMFGNLIRECGVLRSLSETAQNSLVNLVTLLLGITISASMKAD
                     LFVSMDTIKIMVLGLLAFVFDSMAGVLFAKFLNLFLKEKINPMVGGAGISAFPMSARV
                     IQKMGLEADPQNHLLMHAVGANVAGQIASVIAGGVIIGLVPALW"
     gene            3863..4003
                     /locus_tag="PKK75_06885"
     CDS             3863..4003
                     /locus_tag="PKK75_06885"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HNZ83361.1"
                     /translation="MSENVTASLKLMGMGMTAIFLVIIVIYITVLILHRLTNKKPGKK
                     TT"
     gene            4057..5598
                     /locus_tag="PKK75_06890"
     CDS             4057..5598
                     /locus_tag="PKK75_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNZ83362.1"
                     /translation="MDKLAELKEMQDRIKSGGGQKAIDKQHESGKLTARERILKLLDQ
                     GSFVEMDAYVEHRCTNFGMDKKKMPGDGVVSGYGTIDGRLVYIFAQDFTVIGGSLGEM
                     HAAKIVKTQQMALKVGAPLIGINDSGGARIQEGVDALSGYGKIFYNNTIASGVIPQIS
                     VILGPCAGGAVYSPAITDFVFMVKNTSKMFITGPNVIETVTGEKVSAEDLGGAMTHNT
                     ISGVAHFVDETEEDCIEHIKKLLSFLPQNNLETTPVQKVTDNINRIDESLNEIIPENP
                     NKAYDMKSIIFTLADNGEFLEYQPYYAQNIITGFMRLNGRSIGVVASQPKVLAGCLDI
                     NASDKAARFIRTCDAFNIPLLSLMDVPGFLPGTNQEYGGIIRHGAKMLYSFSEATVPK
                     VTVITRKAYGGSYIAMCNRELGADMVMAWPTAEIAVMGPDGAANIIFKKEIDEAEDKN
                     AKRKEKIEEYRDTVANPYTAAARGYVDAVIEPSETRKRIISSFDMLTGKRELRPEKKH
                     GNIPL"
     gene            5608..>5894
                     /locus_tag="PKK75_06895"
     CDS             5608..>5894
                     /locus_tag="PKK75_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83363.1"
                     /translation="MGLFDNLFKKKTVEHEELKIKSTTKEKTTDKDEIAAVIAAAVAA
                     MDEEEEIVAAITAAISIILGKGTDEFIVRNIKLTPEMDSIWAHAGRMKLMR"
BASE COUNT         1962 a          984 c         1307 g         1641 t
ORIGIN      
        1 aatcttgcaa gattgaatta ataggaatca tttaacccca aagtattgca aaaataataa
       61 attcgtatta agatatagca gaaagattaa tgttgaattt aggaggatat tgatggaaca
      121 taagataaca gcagggcttt ccgcaattca taaaagggaa gggactgtac ggcgtacata
      181 tacttattca ggctcaaatg attcattgga ctatctgctg acaacacctg aagtattgga
      241 taacattatc gaggcagcgg caaaaatgct ggatccttta cttcccaacg gatatataac
      301 cgtgggcaaa tatatagaat tatctcatga gcagcctact ttaacattga cagggcatgc
      361 catatcaaca attctaacgg taacggaaat taccggcaat aaagtctttc ttgatttttc
      421 atgtcatgat gaaattggtt tgatatgtaa aggaaaatat gaaagagcta tcgtaaacaa
      481 agaaaaatta atggaatcca catacaggag agcacagagc aaaatgtaat taaattaaca
      541 ggggtgtatt tatgtttatt attgatccat tggctgcaat cattataatg gttgtcattg
      601 ctttcgcatt tacatatttc atcaaaaggg aattaaatta aaaaaagaaa tttggttaaa
      661 aggagttgtc atgaaggatg tttcctttgc gttttcggta tatttattgg gatttgcagt
      721 atcggtatcc attgccggtt taataaaact tattttattc ataataagaa aactttcaag
      781 aaaagaggca aatatttgaa tttcatcagt ctttttcaag gagttggaac actatttgtt
      841 caagagccta aaattgcagc agcaagaatt gtacttattt tacttggtat tttattggtt
      901 tatttaggga aaaaagaaat tttagaacct cttattatga ttcccatggg atttggaatg
      961 gcagcggtta actccggcgt cctatttctc tctgcagcac aaataggaac aatatttatg
     1021 gatcctatgg taacaaatac cgaggagctt atgaccatat tgcaaattga ctggctgcag
     1081 ccgatttata cttttatgtt cagcaacggc ttgattgcct gtttagtttt tatgggcata
     1141 ggggtgattt ctgatgtagg aagtgtttta aaataccctt ttacaggcat gctgatcgcc
     1201 atgtttgccg agcttggaac ggttattact tttccaattg cccactccat gggttttacc
     1261 tacggtgagg ctgcggcaat atctcttata ggcggcgctg acggccccat ggtactgttt
     1321 gcatcccttg cccttgccca ggacttgttc gtaccaatta cagttgttgc ctacctgtat
     1381 ctaagcttaa catatggagg atatccattt ttggtaaggc tgttgatacc aaaagatata
     1441 agagccatag taattaaaac ttcaaaaaca aaacgaaata atattacatc aaatgaaaaa
     1501 ctggcttttg atatatttgc atgcatagtc ctctgcctct tattcccatc ggcgtccccg
     1561 ttgtttttaa gcttcttcct tggcaatgcc ataagagaat caggcataac aaggtatgca
     1621 aagctcctgg aggatgtttt tctttattcg gcaacttttc ttctgggttt gaccttgggt
     1681 gttctatgcg aagcaagcac tattttaaat ccaaaaatac tgcccttatt ggttttgggc
     1741 ataatagcac ttcttatttc tggaataggc ggaattatag gaggttacct tgtttatttc
     1801 ataagcggca aaaacttcaa tcccgtggtg ggaatagcag gtgtttcatg tgtgcccact
     1861 actgccaagg tggcacaaaa agaagtcaac agagttacca agtatagttt tatattggaa
     1921 tatgcaatga gtgcaaatat atgcggtgta ataacaactg caattttaac cggtatttat
     1981 gtaaccttgg tgcctttgct gtgattaaaa aaaggttctt aaaaaataat tgggagataa
     2041 ggaaatgata gtggaatttg aaggtataaa accaactatt gataaaaaaa catacattgc
     2101 cgacggcagc catatcatag gcagggcaac cattaaagaa tattcaagca tatggtttaa
     2161 cactgttgta agagccgata taaactgtat agaaataggc aggtatgtaa atattcagga
     2221 taacagcgtt attcatgttt ccaatgatta ccctgcaatt ataggagatt atgtaacaat
     2281 cggccataat gccacagttc atgcctgcaa aattgaagat cactgcttaa taggcatggg
     2341 ctcaattatt atggatggag ccgttatagg aagagggagc attgttgctg caggtaccgt
     2401 agttaagaaa ggccagcata tacctccatt ttctgttgca gcaggagttc ctgcaaagat
     2461 aataagaaag acagctgaag aagatatatc taaaattcat tctcaagctt taaaatacaa
     2521 agatgtctgg tcaataagat atgggattat gccagatgcc ggcggagaat catatgatta
     2581 cacagatata atatagccaa taaatcaaaa tacatagtta aattagaata tagtcagtat
     2641 ataatacata ataggaaaat ggaattagtt aaaactaaat gcaattataa agatattaca
     2701 caaggagtag aaacatggat tttttaattg aaggaataat ggcagtaaca tggaaacaag
     2761 tagttatgtg gataatcggc ggacttctta tctatctggc aatttcaaag gaactggagc
     2821 cttccttgct gcttcccatg ggttttggag caatacttgt aaacatacct atgtccggcg
     2881 ttttgaacca aacattggat ggaataggac atgtacaggg aattatagac tggctctttg
     2941 aagtgggaat taatgcttcc gaagcaatgc ctcttctttt gttcataggc ataggagcaa
     3001 tgattgattt cggtccctta ttatccaatc ccaggctttt gttgtttggg gcagcagcac
     3061 aatttggaat atttgctaca attacagcag caacacttat gggatatggg ttggctgatg
     3121 cagcatcctt tggaataata ggagcagccg acgggcctac ttcaatactt gtatctcagg
     3181 tgcttgaaag caactacata ggtcccatag cagtggcggc atattcatat atggcattgg
     3241 tacctataat tcagcccttt gccataaaac tggttaccac aaaaaatgaa agaaagatta
     3301 gaatgtctta taatcccaaa agtgtttcca ggatgacaag actcctgttc cctataacag
     3361 taacaataat agcaggactt gtagcgccgg cgtctgtatc attggtagga tttttaatgt
     3421 tcggcaacct gataagagaa tgcggagtgt taaggtcatt gtcggagaca gcacaaaact
     3481 cactggtaaa tttagtaacc ttgcttttgg ggattacaat atcagcgagc atgaaggcag
     3541 atttatttgt atccatggat acgattaaaa taatggtgct gggacttttg gcattcgtat
     3601 ttgattcaat ggcaggagtt ttgtttgcaa agtttttaaa ccttttcctt aaggaaaaaa
     3661 taaatcccat ggtggggggg gcaggaattt cagcatttcc tatgtcagca agggttattc
     3721 agaaaatggg tcttgaagca gaccctcaga accacctgct tatgcatgcg gtgggcgcaa
     3781 atgtagcggg acagatagca tctgttatag caggcggagt aataatcggt ctggtaccgg
     3841 cattatggta ggaggatgag taatgagcga aaatgtaaca gcatctctta aactgatggg
     3901 aatgggaatg acagcaatat ttttggtaat tatagtaata tacattactg tgttaatatt
     3961 gcacaggctt acaaataaaa aacccggcaa aaagacgacc taattctaat tagactggag
     4021 aatatcagat tttaaataca cattacagga ggaactatgg ataaattagc cgagcttaag
     4081 gaaatgcaag acagaataaa gtcaggcggc ggacagaaag ctatcgacaa gcagcacgaa
     4141 agcggaaagc ttacggcgcg tgaaagaatt ttgaagcttc tggaccaggg aagctttgta
     4201 gaaatggatg cttatgttga acacagatgt actaactttg gaatggataa gaagaaaatg
     4261 cccggtgacg gagtggtatc cggatacggt acaatcgacg gcagactggt atacatattt
     4321 gcacaggatt tcaccgtaat cggaggaagc ttgggtgaaa tgcacgctgc aaaaattgtt
     4381 aaaacccagc agatggcgct gaaggtggga gccccgctga ttggaataaa cgattcaggc
     4441 ggtgcaagaa tacaggaagg cgttgatgca ctttcagggt acggtaaaat attttacaac
     4501 aataccattg cttcaggagt aataccgcag atttcagtaa ttctggggcc atgtgcaggt
     4561 ggtgccgtat attctcctgc aataactgat tttgttttca tggttaaaaa tacgagcaaa
     4621 atgtttatta ctggtccaaa tgttattgaa accgttacag gagaaaaggt ttcagcggaa
     4681 gacctgggag gggcaatgac tcacaacaca atcagcggtg ttgctcattt tgttgatgaa
     4741 actgaagaag attgcataga gcatattaaa aagcttctta gctttttgcc ccaaaacaat
     4801 ctggaaacaa caccggtaca gaaagtaacg gataatatta acaggataga tgaaagctta
     4861 aatgaaatta ttccggaaaa tccaaataaa gcatatgata tgaaaagcat aatattcaca
     4921 cttgccgaca acggtgagtt tttggaatat cagccatatt acgctcaaaa tataattacg
     4981 ggatttatga gattaaacgg aaggagcata ggcgtggttg caagccagcc caaggttctt
     5041 gccggctgcc tggatataaa tgcttctgac aaggcggccc ggtttataag aacatgtgac
     5101 gcctttaata taccccttct ttcattgatg gatgtaccgg gattcctgcc cggaacaaat
     5161 caggaatacg gcggaattat tagacatggt gcaaaaatgc tgtattcatt ttccgaagcc
     5221 acagtgccca aggtaaccgt aattacaagg aaggcttacg ggggatcata tattgcaatg
     5281 tgcaacaggg aattgggagc agacatggta atggcgtggc ctacagcaga aatcgcagta
     5341 atgggacctg acggcgcagc taatataata ttcaagaaag aaatcgacga agcagaagac
     5401 aaaaatgcaa agcgaaaaga aaagattgag gaatacagag atacagtggc aaatccatat
     5461 acagcggcag caagaggata tgtggatgca gtgatagagc cctcggaaac acgaaaaaga
     5521 ataataagtt catttgacat gctgacagga aaaagagaac tgagacctga gaaaaaacat
     5581 ggcaatattc ctttgtagga ggggcatatg ggcttatttg ataatttatt caagaaaaaa
     5641 acagttgaac atgaagaatt aaagattaaa agcacaacta aagaaaagac aaccgataaa
     5701 gatgaaattg ctgctgttat agccgcagca gttgcggcaa tggatgagga ggaagaaatt
     5761 gtagctgcaa ttacggctgc aatatccata atactcggaa aaggaacaga tgaatttata
     5821 gtaagaaata taaaactaac ccctgaaatg gactcaatat gggcacatgc agggagaatg
     5881 aaattgatga gata
//
LOCUS       DAOPUT010000083         5869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_13540_length_5869_cov_17.414689, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000083 DAOPUT010000000
VERSION     DAOPUT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13540_length_5869_cov_17.414689"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1196)
                     /gene="dusB"
                     /locus_tag="PKK75_06900"
     CDS             complement(213..1196)
                     /gene="dusB"
                     /locus_tag="PKK75_06900"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNZ83364.1"
                     /translation="MKKLKTGSLELDTNVVLAPMAGVTDITYRTICKEMGAGLVYTEM
                     ISAKGLYYQDRKTEELMKINEKNRPVAMQIFGSDPQIMSYVVEKYIDNRNDVDIIDVN
                     MGCPAPKIVKNKDGCFLMKEPELAGKIISAIKRATKKPVSAKIRSGWSKDSINAAEFA
                     KVLEYNGLDMVTVHPRTRQQYYSGRADYRIINEVKKSVNIPVVGNGDIYSPYDALKMT
                     EETNCDAVMIGRGILGNPWLIMNTVKILNNDYEFYLPSPLDKINMIKRHAAMLVYELN
                     EKIAILEMRKFAAWYMKGMKNSSEIRNSINKITRAEELNKILDEYIEINLK"
     gene            complement(1168..1962)
                     /locus_tag="PKK75_06905"
     CDS             complement(1168..1962)
                     /locus_tag="PKK75_06905"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNZ83365.1"
                     /translation="MYDLNSISSYLSTNIIGQSIIQYDNLDSTYAKAKNIFASCPDGT
                     VVLSENQSECILRFGNEWLCMPDKNIYLSIILKSVNNNYLLPLTDMVGCSSILGSIED
                     LYDLNCTIRWPNDILINDNKISSVKSDITGKGSGIILSLNINVNMEEKLENLKTTSIR
                     LEKGEDVERELLIGTILNKIDYHYDEILSTGKASSAVDIYNKHLLYYNKEVGVIKKGR
                     KTVRKVFVKNIDSEGWLLATNEKGEEEILSPGEIIIQYEETKNRQP"
     gene            complement(1975..3057)
                     /locus_tag="PKK75_06910"
     CDS             complement(1975..3057)
                     /locus_tag="PKK75_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNZ83366.1"
                     /translation="MHKKLFIPGPIEVREDVLNKMATPMIGHRSKDASNLQKNISNNL
                     KKLFFTENEILLSTSSGTGLMEAAVRCCTKERAAIFSIGSFGDRWHEMAVSNGINAEL
                     FKSEPGTITRPEMVDEVLSTGKYDLITITHNETSTGVMNPINEISKVIKKYPEVIFCV
                     DAVSSAGGVKIETDKLGIDICITSSQKALGLPPGLSVCTISERAAEKARYVKNRGYYL
                     DLLQLYDTVKKKDYQYPSTPNLSLMFAMDYQLEKIIKEGLENRFSRHLEMAVHTRSWA
                     KKNFALFADEKYASVTLTAVCNTRNISVKGLNEELGKRGYAISNGYGSLKEKTFRIAH
                     MADLTLEDIKDLLFNIDDILSISQPQ"
     gene            3260..3631
                     /locus_tag="PKK75_06915"
     CDS             3260..3631
                     /locus_tag="PKK75_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ83367.1"
                     /translation="MEFNNNVPIYLQVIERIKQDIVSGKLKPGEKMPSSREYSNELGI
                     NFNTVARVYKELEMEEIVFTKRGLGTFITESDMQIENLRYTMAKERITAFIDGMKQLG
                     YTIKDMVEFIETENNHTEGNE"
     gene            3624..4331
                     /locus_tag="PKK75_06920"
     CDS             3624..4331
                     /locus_tag="PKK75_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ83368.1"
                     /translation="MNKLSILKINNLTKKYYNKTALENVTLDFEPGRIYGLLGPNGSG
                     KTTFMKVVAGLHKQTSGSVLINNEDLSYKTKAYVSFMPTENYLYDSFKVVDILKFYND
                     MYDDFRYDYAVNLINAVNVSTSYKITNLSSGQTGKLKTVLALSRDADIYMLDEPLNGV
                     DVLARDTILDIITKSHNENKIMIISTHLVSEIEKILDTVIFLSEGVVELFGDAEELRQ
                     ERQLSVEGLYKEVFKNA"
     gene            4324..5118
                     /locus_tag="PKK75_06925"
     CDS             4324..5118
                     /locus_tag="PKK75_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83369.1"
                     /translation="MLNLIKYEFIRKYRLISIVLITALALNLLIMTRGPEGSVLFLTL
                     SPFVLYILYFVDIIKMYSDDLNKKTGYMLFMTPSSGYRIISSKLIAAVIEGFGLLLIY
                     FLFFALNGVYILVSLDETVDFSSIISNINKIFSGSLGYGFNIFHLFMLLLTILILIIA
                     FITTIYTAITIRKSLFSEAKFGGLFSFIIFLLINWIVSTLSNGFYNFLSKVSPYYNSL
                     DNIAGAGRFTPETLMMIFIPIIGFSIIQATALSWISGYLLEKKINL"
     gene            5134..>5869
                     /locus_tag="PKK75_06930"
     CDS             5134..>5869
                     /locus_tag="PKK75_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83370.1"
                     /translation="MKRLICILLIIMFLSAGCTTLFEVDEEKVKDVSDKVTEAIINTV
                     GQEKAEKKESRTVDSAGKSTLIVKNSVGDIDIKSHESDDTIINISITAKSGSKQKSEE
                     FIENYNYTLKSEGGAIVVDTSFEEPLSGINLITDLTVYVPSSINNTEVSTNVGTIRLD
                     GLSGNMKIKSNVGDIIIDNSEGSYNLHVDVGDIVIRGSKALENSEFKTNTGGMEMSLD
                     ITKSRRITAETGVGDIKMSINDDSGYH"
BASE COUNT         1792 a         1053 c          998 g         2026 t
ORIGIN      
        1 cttccaaacc ttcagctgta attaaagtct cattattttt catattattg ctccttttaa
       61 taatgtagct tttatacttc catgccatca aatatatatt tcattgtatt tatcatttta
      121 atatattatg ataatctgat gacaaatctt ttaattaatt agaacattat aaacaagtaa
      181 ctgattattg tcaagatttt tgtaactttt gtttatttta ggttgatttc aatatattca
      241 tccaaaattt tattcaattc ttctgctctt gtaattttgt ttatgctgtt tctgatttct
      301 gaagaatttt tcattccctt catataccat gcggcaaatt tgcgcatttc taaaattgca
      361 atcttttcgt tcaactcata aaccagcatg gctgcatgcc tcttaatcat attaatttta
      421 tccaatggtg aaggcaggta aaactcgtaa tcattgttta aaattttaac cgtattcatt
      481 atcagccatg gatttcccaa aatacctctg cctatcataa ctgcatcaca atttgtctct
      541 tctgtcatct taagggcatc ataggggctg tatatatcgc cgttgccaac aacaggtata
      601 ttaacgcttt tttttacctc gtttattatt ctatagtcag cccttcctga ataatactgt
      661 tgtctggttc tcgggtgaac agtcaccata tccaacccgt tatactctaa aaccttcgca
      721 aattcagctg cattgataga atccttactc cagcctgacc ttattttggc tgaaacaggt
      781 ttctttgttg cacgttttat tgctgagata attttacctg ccaattcagg ttctttcata
      841 agaaaacaac cgtccttgtt tttaacaatc tttggtgccg gacagcccat attaacatct
      901 ataatatcaa catcgtttct gttatcgata tatttttcaa ctacgtatga cattatttgc
      961 gggtcgcttc caaatatctg catggctaca ggtctgtttt tttcatttat tttcatcagc
     1021 tcttctgttt ttctgtcctg atagtacaat ccttttgcac ttatcatctc ggtatagaca
     1081 agacctgctc ccatttcttt gcagatagtt ctgtatgtta tgtctgtcac ccctgccata
     1141 ggggcaagta caacgttagt atcaagttca aggctgcctg tttttagttt cttcatattg
     1201 tataattatt tctcccgggc ttaatatttc ttcttcccct ttttcattgg ttgccaaaag
     1261 ccatccttca ctgtcaatat ttttaacaaa aacttttctg actgtctttc tgcccttttt
     1321 gattactccg acttccttat tgtaatacaa caagtgttta ttgtatatgt caactgcact
     1381 ggaagccttt ccggtgctta aaatttcatc ataatgataa tcgattttgt ttaatattgt
     1441 ccctataaga agttctcttt ctacatcttc tcccttttcc agccttatgg atgtagtttt
     1501 taagttttca agtttttctt ccatatttac atttatattt aatgacaaga ttatgccgga
     1561 accttttcct gtaatatcac ttttcaccga tgatattttg ttgtcattta tcagtatatc
     1621 atttggccat ctaattgtgc aattcaaatc atagaggtct tcaattgaac ccaatatgga
     1681 tgagcacccc accatatctg ttaaggggag gagataatta ttattgacag attttaaaat
     1741 aatacttaag taaatatttt tatcaggcat acaaagccat tcattaccga atcttagtat
     1801 gcattccgat tggttctcgg ataatacaac agtaccgtcc ggacaagatg caaatatgtt
     1861 tttagctttc gcatatgttg aatctaaatt gtcgtattga ataattgact ggccgattat
     1921 atttgtactt aaataactgc tgatactgtt taaatcgtac atttggctcc cccattactg
     1981 cggctgagaa attgatagaa tatcatctat attaaataga aggtctttta tatcttctaa
     2041 tgttaaatca gccatgtgtg caattctaaa tgtcttttct tttaaagagc cataaccgtt
     2101 tgaaattgca tagcctcttt ttcccaattc ttcattaagc cctttgacgc ttatgtttct
     2161 ggtattgcat actgcagtaa gcgtaaccga agcatatttt tcatcggcaa ataatgcaaa
     2221 attctttttt gcccagcttc ttgtatgtac agccatttcc aaatgtcttg aaaatctgtt
     2281 ttcaagccct tctttgatta ttttctccaa ttgataatcc atagcaaaca tcaatgataa
     2341 attcggagtt gaaggatact ggtagtcctt ctttttgact gtatcatata attgaagcaa
     2401 gtctaagtaa tatcccctat ttttaacata tcttgctttt tcagcagctc tttccgatat
     2461 ggtgcacacg gatagtcccg gaggcagccc taatgccttt tgcgaggatg ttatgcagat
     2521 atcaatacca agtttatctg tctctatttt tacccctcca gcagagctga ctgcatcaac
     2581 gcagaaaatt acttccggat atttctttat aaccttgctt atttcgttaa tgggattcat
     2641 tactccagtt gacgtttcat tatgggttat tgtgattaaa tcatacttac ctgttgacaa
     2701 tacttcatct accatttctg gccttgtaat ggttcccggt tcagatttga acaattctgc
     2761 gttgatgcca tttgaaacag ccatttcgtg ccatctgtcg ccaaatgatc ctattgaaaa
     2821 tatggctgct ctctccttag tgcagcatcg taccgctgct tccatcagcc ctgtgcctga
     2881 agacgttgac aataatattt cattttcagt aaaaaacagt tttttaagat tattggagat
     2941 atttttttga aggtttgaag catccttgct tctgtggcca atcataggtg ttgccatttt
     3001 atttaaaaca tcttctctta cttctatggg tccaggaata aacagttttt tgtgcatttc
     3061 cccctccgcc tttgtaataa ttgaatttct ttctccattt atataacttt atcaatttta
     3121 tgtcaagcag taattttttt aaaatgttgc gaaaacgtaa taaattattg tgttattttt
     3181 taatattttc ctgttgacaa tgcctatgta ttagtatact attctactat gacactagta
     3241 cacgagagaa ggtgataggt tggaatttaa caataatgta cccatatatt tgcaggtaat
     3301 agaaagaatc aagcaggaca ttgtttcggg aaagcttaag cccggtgaaa aaatgccctc
     3361 atcaagggag tattcaaatg aattaggcat aaatttcaat acggttgcaa gagtatacaa
     3421 ggagctggaa atggaggaaa ttgtatttac aaaaagagga ttgggaacat tcataacaga
     3481 gtctgacatg caaatcgaga atttaagata tacaatggca aaggagcgga ttactgcgtt
     3541 tattgatgga atgaagcaat taggctacac cataaaagat atggtcgaat ttatagaaac
     3601 agaaaataac catacagaag ggaatgaata agttgagtat attaaaaata aataatttaa
     3661 ccaaaaaata ttacaataaa acagcacttg aaaatgtcac tcttgacttt gaaccgggca
     3721 gaatctacgg tttattggga cctaacggca gcggcaaaac cacttttatg aaagttgttg
     3781 caggacttca caagcaaaca agcggctctg tattgataaa caatgaagac ttgtcctaca
     3841 aaaccaaggc ctatgtttca ttcatgccta cagaaaatta tttatatgac tcttttaagg
     3901 tagttgacat attgaaattc tataatgaca tgtatgatga cttcaggtat gattacgctg
     3961 taaatcttat taatgctgtt aatgtcagca caagctataa gataaccaac ctgtcctcgg
     4021 ggcagacagg gaaactgaaa acagttcttg ccttatcaag ggatgcggac atatatatgc
     4081 ttgatgaacc cttaaacggg gttgatgtcc tggcccggga cacaattctg gatattatca
     4141 caaagtccca caatgaaaac aaaataatga ttatatccac acacctggta agtgagatag
     4201 aaaaaatcct tgacaccgtt atttttctta gtgaaggagt ggtagagctt ttcggagatg
     4261 ctgaagagct cagacaggaa agacaattgt ctgtggaggg actgtacaag gaggtattta
     4321 aaaatgctta atttgataaa gtatgaattc atcagaaagt atagactgat ttctatagta
     4381 cttataacgg ctcttgcttt gaatttactc ataatgacaa gaggccctga aggaagtgta
     4441 ttattcctta ccttatcgcc ttttgtgtta tacattctct attttgtaga catcataaaa
     4501 atgtacagcg atgacctaaa taagaagacc ggttacatgc ttttcatgac tcccagcagc
     4561 ggatatagaa taatttcgtc gaaacttata gctgctgtca ttgagggttt tggactgctg
     4621 cttatttatt tcctgttttt cgcattaaac ggtgtctaca ttcttgtatc ccttgatgaa
     4681 actgtagatt tctccagcat tatcagtaat atcaacaaaa tattttcagg cagtttgggt
     4741 tacggcttca acatattcca tttattcatg ctgctgctga ccattcttat attgataatt
     4801 gcttttataa caaccattta cactgcaata acaataagaa aaagcttgtt ttctgaagca
     4861 aaatttggag gactgttcag ctttataata tttcttttaa ttaactggat agtatcaaca
     4921 ctgtcaaatg gattctataa tttcttaagc aaggtatcac cctattacaa ttccttggac
     4981 aatatagccg gagcaggaag atttacacct gaaacactaa tgatgatttt tataccgatt
     5041 atcgggtttt caataattca ggcaacagcc ctgtcctgga tttcaggata tcttttagaa
     5101 aagaaaataa atttataaag gcaggagagg aaaatgaaaa gattaatttg catcctatta
     5161 ataattatgt ttttgtctgc aggctgtact actttgttcg aagttgatga agaaaaggtt
     5221 aaagatgttt cagataaggt gactgaagct attatcaata cagtgggaca ggaaaaggct
     5281 gaaaaaaaag agtctcgcac agtggactcc gcaggtaaaa gtactttgat cgtaaaaaat
     5341 tctgtaggag atatagatat taaatcccat gaatctgatg atacaataat aaatataagc
     5401 attacagcta aatccggctc aaaacaaaaa tcagaggagt tcattgaaaa ttataattac
     5461 actttaaaat cggaaggcgg agccatagtg gtagacacat catttgaaga gcctttaagc
     5521 ggtataaacc ttataacgga tttgactgta tatgttcctt cgtctattaa taatacggaa
     5581 gtttcaacaa atgtaggcac tatccggtta gatggcttat caggaaacat gaagattaaa
     5641 agcaatgtgg gggatattat tatagataac tcagaagggt catataattt gcatgttgat
     5701 gtgggagaca ttgttataag ggggtcgaag gccttggaga attcagaatt taaaacaaat
     5761 accggaggca tggaaatgtc ccttgacata acaaaatcac gcaggattac agcggaaaca
     5821 ggagtaggag atattaaaat gtctataaat gatgattcag ggtatcatg
//
LOCUS       DAOPUT010000084         5860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_13556_length_5860_cov_19.482171, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000084 DAOPUT010000000
VERSION     DAOPUT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13556_length_5860_cov_19.482171"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /gene="sigK"
                     /locus_tag="PKK75_06935"
     CDS             complement(<1..683)
                     /gene="sigK"
                     /locus_tag="PKK75_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HNZ83371.1"
                     /translation="MIEELLQILLPLSIFIFGYITNGSSFPQPLNKDEEAILIEKSNK
                     GDIEARNILIERNLRLVAHVIKKYKTVGIDQEDLISIGTIGLIKAVSTFDDKKGIRLA
                     TYAAKCIDNEVLMTIRSEKKLKKEVSLQGPLGTDKEGNNIYLIDIIKYEGEEIVGRLD
                     KEEKIKALDVILDRTLQKRERFIIEMRYGIRNKEHKTQREIAKLLGISRSYVSRIEKK
                     ALKKLHDALV"
     gene            complement(808..1506)
                     /locus_tag="PKK75_06940"
     CDS             complement(808..1506)
                     /locus_tag="PKK75_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83372.1"
                     /translation="MNNYRNNMPHYDSLPHHYNPMGIDEHKNFNNNRMLLASAMPGMP
                     GMPEDMLRIPFIPDTEIPLEIDGDMPALPGMPQQGIPGLIPPWMPQMPRMPGMPQMPV
                     MQQPQMPQMPGMPGMPQMPGMPGMPQMPGMQQPQMPMMPGMPGIPRMPQAPGMPQAPG
                     MQQPRMPMMPQMPQTPQMPQMPGMPPEDGNMYPMMPGMPPITCEQLREMMTRMNCPMN
                     GEEGGATPPADEDM"
     gene            complement(1868..5242)
                     /locus_tag="PKK75_06945"
     CDS             complement(1868..5242)
                     /locus_tag="PKK75_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ83373.1"
                     /translation="MKQAKHFTAVILLISMFLTMYTPLQAFGENVEPLQFDGSQSTWA
                     EPEIKEAYDMSLTYPAVTNNFNKYITREEFCTLVIKLYEKLTGQIPKYTDNPFKDTTN
                     TEILKAHSLGIVKGISADTFAPLNNITRQEMCVMILRALNVSVQGLNTSPGEFPFKDA
                     DKIAPWAIDAMKFAYKNGIMKGLSAETIDPLSNTPREQAIILLLRTYKKFSITTVQSI
                     EEAGAVRFNKPPSVSKNIREKFEFTMNSNNIFFPKFDKKLELFVSNKAGKPSTLPIIS
                     SLDNSPVLFASLNNHVITYSSLINPIRPINPIDPIETISPIIKDPIIIKPNLPDLVIM
                     HPAVKERTNTPMYINSDFGAFIDKTENSVRWFTYKLNNIRGFNTVSKIVWQVSETQFV
                     GTKDVFRTEKGIVLSGDIMPSSKEFSIDFSKVKSLAQIKKPIPQEQKTYYVRAVPVDA
                     LGNPIGDPGQGIAVLYGIPVMSTRESTDASFEIWSTKSHMGNNTTGEFKDVPVHDPFS
                     NGIIEFNPNEANPRIFHFHGLDENTVKIYVQVYSDKFDKNQSYLDYDNLVYEREYYID
                     DMVPVYPHLNFNPSATVDFKEFGKTDMKADEYIRYYLRAVAITEDIEPGKLKAVYSDI
                     ITVDYGFGSPVKFISLPVPQSKYDKVQMINYSLPSVEIKEYTAIDWADPDYLSRYYVF
                     REPKPTEIIYTWMNADGKILKPYKYYVITKELGNFSSSGGFDFDLNTSYEMMPQEFKT
                     KYEKLIKEVLPIGSKIYIPVPKEVDKPWYQEIYEGVVNFFKDLVSTIIKIYAKITETY
                     QALKTKLIMAVANLCPIESLRDEFAKALTALVDYGLSSIGIPPKFPNFEQLAEGNIAY
                     LAQVALTEAGVPASDITEEMTKEAAKGVVGYFKEADKESYDENPVKSPFLKLDPQSLY
                     QPACLEVELENNTDYPTVPGTLDLNVQFKLKEQGLFATSYDPVGLNLVADNNQYGYGS
                     SMAVLNATDYWNHFVYGLNGYTVNYKYGEEAVYEVFEPKVDVKIPVIPAHNSVTLKIF
                     LDPAGVAINNRYPDGENYRFEDFYNMYQNNGGADYTWFEVTTCFPEARKYYSDEYAKK
                     GTIMVLDPKTDYRYHMDTSYSANLGRKIQMPVNIDW"
     gene            5491..>5860
                     /locus_tag="PKK75_06950"
     CDS             5491..>5860
                     /locus_tag="PKK75_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ83374.1"
                     /translation="MHLTNLHTHSLYCDGSCSPEEMVLSAINNNFDSLGISSHGPVNK
                     ETDWNIKQDKVEEYIEEVNRLKEKYKDKIEIFLGMELDYIPGAGFTDLCLSLMKRLDY
                     YIGSVHYLGTLKNGYMWTVDY"
BASE COUNT         1652 a         1093 c         1076 g         2039 t
ORIGIN      
        1 accaaggcgt catggagttt tttaagggct tttttttcaa tccttgacac gtaagatctt
       61 gatataccga gaagtttggc gatttctctc tgagttttat gctctttgtt tcttattccg
      121 tagcgcattt caataatgaa tctttctctt ttttgaagtg tcctatctaa tattacatct
      181 aaagctttta ttttttcttc tttatccaat ctccctacta tttcttcacc ttcgtatttg
      241 ataatatcaa tgagataaat attattgcct tccttgtctg ttcccaaagg cccttgaaga
      301 gacacttcct tcttcagttt tttttcgctt cttatggtca taagcacctc gttgtcaatg
      361 catttggcag cataggtggc aagtcttatt ccttttttat catcaaaagt tgaaacagct
      421 ttaatcagac cgattgttcc tatggatatt aagtcttcct ggtcaattcc tacagtctta
      481 tactttttta taacatgtgc cacaaggcga agatttcttt ctataagaat atttcttgct
      541 tctatatcac ctttatttga tttctctatt agtattgctt cttcgtcctt gtttaaaggc
      601 tgcgggaaag agcttccgtt tgttatgtaa ccaaaaataa atattgatag cggtaacaga
      661 atttgcaata gttcttcaat caaaaaaaca cccccaaagt tatacattat atgcaagata
      721 tttaaaaaac tgtatgtaca ctttaaaaac tttttcaatg taatatttaa cagcatttct
      781 gctattaaat attacattgg ctatttgtta catatcctca tccgcaggcg gcgtcgcacc
      841 gccttcttcg ccgttcatag ggcagttcat gcgtgtcatc atttctctta attgctcaca
      901 ggtaataggg ggcattccgg gcatcatggg gtacatattg ccatcctccg gcggcatacc
      961 cggcatttgc ggcatctgag gtgtctgcgg catttgcggc atcatgggca ttcggggttg
     1021 ctgcatcccc ggcgcttggg gcatccccgg agcttgaggc atccttggaa ttcctggcat
     1081 ccccggcatc atgggcattt gaggttgctg cattccgggc atctgaggca tcccgggcat
     1141 tccgggcatc tgtggcattc cgggcatccc cggcatctgt ggcatttggg gttgctgcat
     1201 tacgggcatc tgaggcatcc ccggcattct gggcatctgt ggcatccacg gtggtattaa
     1261 acccggaatc ccctgttgcg gcatcccagg cagtgctggc atgtctccgt caatttccaa
     1321 gggtatttcc gtatccggaa taaatggtat tcgtaacata tcttcaggca ttccgggcat
     1381 tccgggcatt gcgcttgcta gcaacattct gttattattg aaatttttgt gctcgtcaat
     1441 gcccattgga ttataatgat gcggcaatga atcgtagtgc ggcatattat ttctataatt
     1501 attcatacta acctccattt aatagatttt ccttatatta atatacgttt gaaaaaaata
     1561 ttagtgaatt aaaaataaag aatcttttaa ttattttatg atgataggat tgatatattt
     1621 tgcttctgtt tcttatactt tatttcttag cttgagataa tttcacatta gtgaatatat
     1681 catataattt ggaaactgct cttgacttca aatcagcaga tatatgggaa taaatgtcaa
     1741 ggattgtttg tatagaattg tgctcaagaa aactccccac cgctgatatt attcattggt
     1801 gaggagtttg ttgttcactt taattattat gctcacaatg attccctatc cggtaatgat
     1861 taattcttta ccagtcaata tttacaggca tctgaatttt tcttccaagg tttgcagaat
     1921 aggaagtatc catatggtat ctgtaatcag tcttagggtc aagaaccata attgttcctt
     1981 tttttgcata ttcatcactg taatattttc ttgcttcagg gaagcatgtt gttacttcga
     2041 accatgtgta atctgctccc ccgttatttt gatacatgtt gtaaaaatct tcgaaacggt
     2101 aattttcacc atcaggatac ctgttgttta ttgcaactcc agcagggtct aagaaaattt
     2161 ttaatgtaac tgaattatgg gcaggaatta ccggaatctt cacatctact ttcggttcaa
     2221 aaacttcata tactgcttct tcaccatact tgtaattaac tgtataaccg tttaatccat
     2281 atacaaagtg attccaataa tctgttgcat tcaaaactgc catggaggaa ccatagccat
     2341 attgattatt atctgcaaca agattcagcc ctacaggatc atatgatgtt gcaaacaatc
     2401 cttgttcttt taacttgaat tgaacattca aatccaaagt tccgggtaca gttgggtagt
     2461 cagtattatt ttcaagctct acctccaggc atgccggctg gtacaaagat tgtggatcaa
     2521 gttttaaaaa tggagacttt acagggtttt catcatatga ttctttatct gcttccttga
     2581 aataacctac tactccttta gctgcttctt tggtcatttc ctcagttata tcgcttgcag
     2641 gaacacctgc ctcagtaagt gcaacttgag ctaaatatgc tatatttcct tctgccagct
     2701 gttcaaaatt cgggaattta ggagggattc caatagatga aagtccataa tcaaccaggg
     2761 ctgtcagcgc ttttgcaaat tcatccctta aactctcaat tgggcataga tttgcaactg
     2821 ccataatcag ctttgtttta agtgcttgat acgtttcagt aatcttggca taaatcttaa
     2881 taatagtaga taccaaatct ttaaagaaat taacaactcc ttcgtatatt tcctgatacc
     2941 atggcttatc tacttctttc ggaacaggta tatatatttt acttcctata ggcagcactt
     3001 ccttgataag tttttcgtat ttggttttga attcttgagg catcatttca taagaagtat
     3061 ttaaatcaaa gtcaaagccg ccggaactgc tgaagtttcc taattcttta gttatgacat
     3121 agtatttata aggttttaaa attttgccgt cagcattcat ccaggtatat ataatctcgg
     3181 ttggttttgg ttctctgaat acatagtatc ttgataaata atcagggtca gcccagtcga
     3241 tagcggtata ttctttaatt tcaacgcttg gtaatgaata atttatcatc tgtaccttgt
     3301 catacttaga ctgaggtact ggaagagata tgaattttac agggcttcca aaaccataat
     3361 ccactgtaat tatatctgaa tagacagctt tcagcttgcc cggctctata tcttcagtaa
     3421 tagccacagc tcttaaataa tatctgatat actcatcagc cttcatatcg gtcttcccaa
     3481 attctttaaa gtcgacagta gctgatggat taaaatttaa atgaggataa acaggaacca
     3541 tatcatctat gtaatattct ctttcatata caagattatc ataatctaag taactttggt
     3601 tcttgtcaaa tttatcgctg taaacctgga cataaatttt cacggtattt tcatccaaac
     3661 catgaaaatg gaaaattctc ggattggctt cattgggatt gaattctatt atgccgttgc
     3721 taaaggggtc atgtaccggc acatctttaa attctcctgt tgtgttgttc cccatatgtg
     3781 atttagtaga ccatatttcg aacgaagcat ctgtagattc ccttgtggac ataaccggaa
     3841 taccatacaa tactgcaatc ccctgcccag gatctccgat gggatttcca agtgcatcaa
     3901 cgggaacagc cctcacatag taggtttttt gttcctgagg aataggtttt ttgatttgcg
     3961 ccaaggattt aactttgctg aagtctatgg agaattcctt ggatgaaggc attatatctc
     4021 cggacaagac tattcctttt tcagttctga acacatcctt tgttccaaca aactgtgttt
     4081 cagatacctg ccatacaatc tttgaaacag tattgaatcc tcttatattg tttagtttat
     4141 aggtgaacca tctgactgag ttttcggttt tatcgataaa agccccaaag tcagaattta
     4201 tatacattgg agtatttgtt ctttctttaa cagcaggatg cattattact aagtcaggta
     4261 aatttggttt tataataatc gggtccttta taatcggaga aatcgtctca atagggtcaa
     4321 ttgggttaat tggtctaatg ggattaatta aagatgaata agtaataaca tggttgttaa
     4381 gtgaagcaaa taaaacagga ctgttgtcca atgaagaaat aataggtagc gttgaaggct
     4441 tacctgcctt attggaaaca aaaagctcta atttcttgtc aaacttagga aagaatatat
     4501 tgtttgaatt cattgtgaat tcaaattttt ccctgatgtt tttagagact gacggcggct
     4561 tgttgaatct taccgctcct gcttcttcaa ttgactgaac agttgttatg ctgaatttct
     4621 tataggttct gagaagcagt attattgcct gctctctggg tgtgttggaa agaggatcta
     4681 ttgtttcagc ggatagtccc ttcattattc cgtttttata agcaaatttc atggcatcaa
     4741 tagcccaagg cgcaatttta tctgcatcct tgaaaggaaa ttctcctggt gaagtgttaa
     4801 gtccttgcac cgaaacattc aatgctctta aaatcattac acacatttct tgtcgtgtaa
     4861 tattatttaa aggtgcaaaa gtatcggcag atataccctt aacaatacct aaagaatgag
     4921 ccttaaggat ttctgtgtta gttgtatcct taaaaggatt atcggtatat ttaggaatct
     4981 gtccggtgag tttttcataa agtttgatta ccagcgtaca gaattcttca cgagttatgt
     5041 acttgttaaa attgtttgtc actgcaggat atgtgagaga catatcatag gcttctttga
     5101 tttcaggttc agcccaggtg ctctggctgc cgtcaaattg gagcggctca acgttttctc
     5161 caaaagcttg gagaggcgtg tacattgtaa gaaacattga aattaatagt attacagcgg
     5221 taaagtgttt agcttgtttc atgatctcac acccttcata tttattagta ttattggtta
     5281 ttattacctt gatttcatta tattcttact tgttatttag tcaagaaaat tataaacatt
     5341 ttatactttg caaagtggtg gagttgttac cgtcgatgga gacgcaataa aaaataaaga
     5401 attctttact attttaataa attcatataa aatgtaacga gatagatata ctttactgct
     5461 atagatggcg ggtacaacgg gaggaaacaa atgcatttaa ctaatttaca tactcacagt
     5521 ctttattgtg acggcagctg cagccccgaa gagatggttc tatcggcaat taacaataac
     5581 tttgattcgt tgggcatttc atcacacggg cctgttaata aggaaactga ctggaatatt
     5641 aaacaagaca aagttgaaga atatatagaa gaagtcaata ggctgaaaga aaaatacaag
     5701 gataaaattg aaattttcct tggtatggag ttagattaca tacctggtgc agggtttaca
     5761 gacctgtgtt tgtctcttat gaaaagactt gattactata tcggctcagt gcattatttg
     5821 ggaactttaa aaaacggcta tatgtggact gttgattata
//
LOCUS       DAOPUT010000085         5820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_13686_length_5820_cov_19.353339, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000085 DAOPUT010000000
VERSION     DAOPUT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13686_length_5820_cov_19.353339"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /gene="whiA"
                     /locus_tag="PKK75_06955"
     CDS             complement(<1..930)
                     /gene="whiA"
                     /locus_tag="PKK75_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HNZ83375.1"
                     /translation="MTFSSRIKDELTRTEIIDAFSAKAEVAAFVRTLGYITIKGLNKV
                     EFEFLTENAAVARRIFKVLKTAYDINAEVSVTKSNRLKKQNNYIIRIDEKLTRQFLKD
                     TGMVRNNDFNIMNFNYDVPEGFIGSDLSKRAYIKGSFMGSGSISNPEKSYHAEFVSSR
                     EAQSKTLQMLLKKYGINSKNIYRKNNYVTYIKESEQISDLLALMGANNAVLDFENIRA
                     VKETRNQINRVINCETANLDKIVDTSMRQINNIKILKKHKVIDQLPDNLRELAYLRLK
                     HSNASLKELGQMLDPPLGKSGVNHRLRKIEEIAE"
     gene            complement(939..1364)
                     /locus_tag="PKK75_06960"
     CDS             complement(939..1364)
                     /locus_tag="PKK75_06960"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ83376.1"
                     /translation="MIEEISAGGIVFFKNSILMLRKFNGDWVLPKGRVEDNESLKETA
                     MREVYEETKAKVIPIKYLGKISYEFSRTCYVDKIHIKKEVHWYLMIARNMNCTAQKNE
                     GFVEAKFLHFEKSLEIARYDDERKIIKKAIDEIKFRSYE"
     assembly_gap    1490..1589
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1590..2715)
                     /locus_tag="PKK75_06965"
     CDS             complement(<1590..2715)
                     /locus_tag="PKK75_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNZ83377.1"
                     /translation="MDKIKLAAISAFGLEAVVKRELADLGYENIVTDNGWMYFDAEIQ
                     DICRTNINLRCADRVMLIMGQFEALSFEELFDRTYELPWENWITKDGRFTVKGKSVKS
                     KLYSTPDCQRMVKKAVSKKLGEYYDTDWMPESGAEFTILVSISKDMATISIDTTGARE
                     GLFKRGYREKSMTAPIKETMAAALVKLSYWNKDRVLYDPMCGSGTIAIEAAMIGRNIA
                     PGLNRSFASMEWPLVKEEYWKNEKIEARKKIDFDSEIKIYASDVSEKAIRIARENAVE
                     AGVDDCIEFFVKDVTHLEKPMCSYGVLITNPPYAERIGDEEHLAGIHKKLGQVFGRDK
                     TWSVYVITSSVNFEKEFGRKADRKRKLFNGDMRVDYFQYYG"
     gene            complement(2771..3919)
                     /locus_tag="PKK75_06970"
     CDS             complement(2771..3919)
                     /locus_tag="PKK75_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNZ83378.1"
                     /translation="MNTDEIIKNSLRENKFIYGIYTTPRIKSQYKKITVRVIAIKDKP
                     YIQLEKFTDTQVFHENLAFEDSFDVLLHLVNEYRNINLFTVDADYQILSSKKGSMKIT
                     RKDPSKNQRAEDHNREKKYIINENQPCDFLILLGVMSPDGRVYTKKYDKFKQINKFLE
                     IVDDSLAGKDIRDDFTIIDFGCGKAYLTFALYYYFYHVRKIKVKITGLDLKQEVIEFC
                     NETAKTLNYENLQFVYGDIRDFEYKNKVNMTVTLHACDTATDAAIVKAIFWDCDIILS
                     VPCCQHEFYDKIENESLNSMLKHGLIKERISSLVTDSLRGLFLETKGYKVQLMEFISM
                     DHTPKNILIRALKANKNIQSAEGEYEDFKKFWNLKDLFIVNYYNKINN"
     gene            complement(3980..5656)
                     /locus_tag="PKK75_06975"
     CDS             complement(3980..5656)
                     /locus_tag="PKK75_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNZ83379.1"
                     /translation="MSEYNNENESRESVNFIQKIINEDLEKGTYGNRVHTRFPPEPNG
                     YLHVGHAKSICLNYGLAVKNRGIFNLRFDDTNPAKEDETFVESIIADVKWLCPDWEER
                     IFYASDYFGKMYDYAVELIKKGLAFVDDLSADEIREYRGTLTEPGKESPYRNRSIEEN
                     LDLFARMKKGEFEDGARVLRAKIDMASPNINMRDPVIFRILHETHHNTGDEWCIYPMY
                     DYAHPLEDAIEGITHSICTLEFEDHRPFYDWLLTNIDDFKDAPTKQIEFAKLFLSKTM
                     MGKRYLKQMVEEGYVDGWDDPRMPTIAGLRRRGYTPESIQNFCEAIGVSKAQSVVDIA
                     MLEFAVRDDLGPKAPRTMAVLDPIKLIITNYPEDKVEYVETENNPDNPDMGTRTIPFS
                     REVYIEREDFMEYPPKKYFRLYPGNEVRLRNAYFVKCVDFVKDEEGNITEVHCTYDPE
                     TKSGSGFTGRKVKGTLHWVSAKHSIDAEVRLYDYMMEDEEYDKSNFLEKLNPESKIVL
                     KNCKLEPSMKEFKAGDRFQFLRHGYFMLDKDTTQDHLVFNRIVPLKSSYK"
BASE COUNT         1481 a         1229 c          921 g         2089 t
ORIGIN      
        1 ttcagcaatt tcttctattt ttcttagtct gtgatttact cctgatttgc caaggggagg
       61 atccagcatt tgtcccagtt cctttaaact tgcgttgctg tgtttcagcc tcaaatacgc
      121 cagttccctt aaattatccg gaagttggtc tataacctta tgttttttta atatcttgat
      181 attgtttatc tgcctcattg acgtgtccac gattttgtca agatttgcag tttcgcaatt
      241 tatcaccctg tttatctggt ttcttgtttc cttaacagct cttatgtttt caaaatccaa
      301 aacagcgtta ttggctccca ttagagccag caaatcggaa atttgctcgc tttccttaat
      361 ataggttaca tagttgttct ttctgtaaat attcttgctg tttattccat attttttcaa
      421 aagcatttga agagtcttgc tttgtgcttc cctgctgctc acaaattcag catgataaga
      481 cttttccggg ttgcttattg aaccacttcc cataaatgaa ccttttatat aggctctttt
      541 gcttaagtcg cttcctatga atccctcagg tacgtcatag ttgaagttca ttatgttgaa
      601 atcattgttc ctgaccatcc cggtgtcttt aagaaattgt ctggttaatt tttcatcgat
      661 tctaattata taattattct gtttcttaag tctgttgctt ttagtaacac ttacctccgc
      721 atttatatca taggctgttt ttaaaacctt gaatatccgt cttgcaacag ctgcattctc
      781 agtcaaaaat tcgaattcca ccttattgag gcctttaatt gtgatatacc ccaaggttct
      841 tacaaatgct gcaacctcag ctttagcact aaaggcatcg attatttctg ttcttgtgag
      901 ttcatcttta attcttgaag aaaatgtcat tatatcacct attcatatga gcggaactta
      961 atttcatcta tagctttctt aatgattttt ctttcatcgt catatcgggc aatttccagt
     1021 gacttttcaa agtgcagaaa ttttgcttca acaaatcctt cgtttttttg ggcagtgcag
     1081 ttcatattcc ttgcaatcat caaataccaa tgaacttctt tttttatatg tattttatca
     1141 acataacagg tgcggctgaa ttcatagcta attttgccca ggtatttgat aggaatcact
     1201 tttgctttag tttcctcata tacttcacgc atggcggtct ctttcagcga ttcgttatct
     1261 tcaacccttc ctttcggaag aacccaatct ccgttaaatt tccttaacat caatatgctg
     1321 tttttaaaaa aaacaattcc tcctgcgctt atttcttcta tcatatttgc ccgccttcta
     1381 atattgtagt ctttataata tcacaatatt aaaaaaaatc aaacacatat ttacatattt
     1441 taagtttaca ataaatgtac atatatttca atgttttttg taataattan nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cccgtaatac tgaaagtaat caactctcat
     1621 gtcaccattg aagagcttcc ttttcctgtc tgctttcctt ccgaattcct tttcaaagtt
     1681 aacagaggaa gtgattacat atactgacca ggttttatcc ctgccgaata cctggccaag
     1741 ctttttatga attcccgcaa gatgttcttc atcaccgatt ctttctgcat aaggggggtt
     1801 tgtgattaga actccgtagg agcacatggg tttttccagg tgggttacat cctttacaaa
     1861 aaattctata cagtcgtcaa ctcctgcttc aactgcattt tcccgtgcga ttcttattgc
     1921 tttttcactt acatcagaag catatatctt gatttccgaa tcaaaatcta tcttttttcg
     1981 tgcttcaatc ttctcgtttt tccaatactc ttctttaacc aaaggccatt ccatggaagc
     2041 aaagctgcga ttcaagcccg gcgcaatatt tcttcctatc attgctgcct caatggctat
     2101 tgtcccggaa ccgcacatgg ggtcgtaaag cactctgtcc ttattccaat agctcagctt
     2161 taccagggct gcagccatag tttcctttat aggggcagtc attgattttt ctctgtatcc
     2221 tcttttaaaa agaccttccc tggctccggt ggtatcaatt gaaattgttg ccatatcctt
     2281 gctgattgaa accaggattg taaattcagc tcctgattcc ggcatccagt ctgtgtcata
     2341 atattcgccc agttttttcg aaacagcctt tttaaccatc ctctgacagt ctggagtgct
     2401 gtatagcttt gatttgacgg attttccctt cacagtgaac ctgccgtctt ttgtaatcca
     2461 gttttcccaa gggagttcat aggttctgtc aaatagttct tcaaagctca atgcttcaaa
     2521 ttgccccatt ataagcatta ctctgtcggc gcacctcaaa ttgatatttg ttctgcatat
     2581 atcctgtatt tctgcatcaa aatacatcca gccgttatca gttactatat tttcataacc
     2641 caagtctgcc aattccctct ttaccactgc ttcaagacca aaagccgata ttgcagctaa
     2701 ttttatttta tccatatact tcctccataa gggaacattc cttcattcat ccctgttgtt
     2761 ccgggttgtt tcaattatta atcttattat aatagtttac tataaacaaa tctttgagat
     2821 tccagaactt cttgaaatct tcatattccc cttctgccga ttgaatattc ttatttgcct
     2881 taagggctct tatgagaata tttttggggg tatggtccat tgaaataaat tccataagct
     2941 gaactttata tcccttggtt tccaaaaaca agcctctcaa tgaatcggtg accaaagagg
     3001 atattctctc ctttattagt ccgtgcttaa gcatggagtt taaactttca ttttcaattt
     3061 tatcgtaaaa ttcatgctgg cagcaaggca cagacaaaat aatatcgcag tcccagaaaa
     3121 ttgcctttac aatggctgcg tctgttgccg tatcgcaggc atgaagagtt actgtcatat
     3181 ttaccttgtt tttatattca aaatcgcgta tgtcgccata cacgaattga agattttcat
     3241 aattcagggt cttggctgtt tcattgcaaa attcaatgac ttcttgtttt aagtcaagtc
     3301 ctgtgatttt cacctttatt tttcttacat gataaaaata ataataaagg gcaaatgtaa
     3361 gatatgcttt tccgcagcca aaatcgataa tggtaaagtc atcccgaata tcttttccag
     3421 caagggaatc atcgacaatt tcaagaaatt tatttatttg tttgaatttg tcgtatttct
     3481 ttgtataaac cctgccgtcc gggctcatga ctccaagcag tatcagaaag tcgcagggct
     3541 ggttttcatt tataatatat tttttctccc tgttgtgatc ttctgctctt tgattctttg
     3601 aagggtcctt ccgtgtgatt ttcatactcc cttttttgct tgacagtatc tggtaatctg
     3661 catcaacagt aaataagttt atattgcggt attcgtttac caggtgaagc aaaacatcaa
     3721 aagaatcctc aaaggctaag ttttcgtgaa atacctgggt atcagtaaac ttctcaagct
     3781 gaatataagg cttgtctttt attgcaatta ccctgacagt tattttcttg tattgagact
     3841 taatccttgg ggtagtataa ataccgtata taaacttatt ttctcttaaa ctgtttttaa
     3901 ttatttcgtc agtattcaat atatctcctg tggtgttgtc atccgccgaa agcgaaggaa
     3961 aaatagggtt agggcatctt tatttgtagc tgctcttcaa cgggactatt ctgttaaaca
     4021 caagatggtc ctgggttgta tccttgtcaa gcataaaata tccgtgcctt aaaaactgga
     4081 atctgtcgcc tgctttgaat tccttcatag aaggttcaag tttgcagttt tttaacacga
     4141 tcttggattc gggatttaat ttctccagga aatttgattt atcgtactcc tcatcctcca
     4201 tcatgtagtc ataaagtctt acttctgcat caatggagtg ttttgcagaa acccagtgga
     4261 gagtcccctt tacttttctg cctgtaaagc ctgagccgct ctttgtttca ggatcgtatg
     4321 tacagtgaac ttcagttata ttgccttctt catcctttac aaaatcaaca cactttacaa
     4381 agtatgcatt tctaagcctc acttcattac cgggatagag cctgaaatac ttttttggag
     4441 gatattccat gaaatcttcc ctttcaatat atacttccct tgaaaaagga atcgtgcggg
     4501 tacccatgtc cggattatcc gggttgtttt ccgtttccac atattcaacc ttatcttcgg
     4561 gatagttggt tattataagc tttatggggt ctaatacagc cattgtcctg ggagctttag
     4621 gtcctaaatc atctcttacg gcaaattcaa gcatggcaat atcaaccacg ctttgagcct
     4681 ttgatactcc tattgcttca cagaaattct gtattgattc aggagtatag cctctccttc
     4741 tgagtcctgc tatggtgggc atacggggat catcccagcc gtctacgtat ccttcttcaa
     4801 ccatttgctt taaatatctt ttgcccatca tagtttttga taaaaacagc ttggcaaatt
     4861 caatttgttt tgtaggtgca tctttaaaat catcaatatt tgtcaatagc cagtcgtaaa
     4921 atggtctgtg gtcttcaaat tccaaggtgc aaatcgaatg tgtaatacct tctatggcat
     4981 cctccagggg atgggcatag tcatacatgg gataaatgca ccattcatca ccagtattgt
     5041 gatgggtttc atgaagtatc cgaaaaatta ccgggtctct catgttgata ttcgggcttg
     5101 ccatgtcgat tttagccctt aatacccttg ccccgtcttc aaattcacct ttcttcattc
     5161 ttgcaaacaa atccaggttt tcttcaatgc ttctgtttct gtaggggctt tccttgcctg
     5221 gctcagtcaa tgtgcctctg tattcacgaa tttcatctgc agacaagtcg tccacaaagg
     5281 ccagtccttt ctttatcagc tcaaccgcat aatcgtacat ctttccaaaa taatctgacg
     5341 catagaaaat tctttcttcc caatcggggc aaagccactt gacatccgca ataatagatt
     5401 ctacaaatgt ttcgtcttct tttgccggat ttgtatcatc aaatcttaaa ttgaatattc
     5461 ccctgttttt tacggctaat ccgtaattca ggcagatgga cttggcatgg cctacatgca
     5521 gatagccgtt aggctccgga ggaaatcttg tatgtaccct gttcccgtag gtgccttttt
     5581 ccaggtcctc gttaattatc ttttgaatga aattaacgga ttctctgctt tcattttcgt
     5641 tgttatattc gctcattttt tccctcctgc tcttctttcc aaagtatact acattttata
     5701 gtaaaataat aactttattg atatttcttt acattaaatc cctaactctg caagcccggg
     5761 attatttaac taaactttat tataattaat gaagctttat aattcaagtt aattattcca
//
LOCUS       DAOPUT010000086         5779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_13828_length_5779_cov_17.798218, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000086 DAOPUT010000000
VERSION     DAOPUT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13828_length_5779_cov_17.798218"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..423)
                     /locus_tag="PKK75_06980"
     CDS             complement(82..423)
                     /locus_tag="PKK75_06980"
                     /inference="COORDINATES: protein motif:HMM:NF022953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HNZ83380.1"
                     /translation="MGKKYIFLIVLLVLALIVISVVNKLITGSDEEKVSFNARVLENN
                     GNFILVEPEEGSSELNSSDKIVVRVRGENNVLKDLSEFTVGSKVKITYDGMIMESYPA
                     QINAYSVELIK"
     gene            complement(518..>1214)
                     /locus_tag="PKK75_06985"
     CDS             complement(518..>1214)
                     /locus_tag="PKK75_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNZ83381.1"
                     /translation="NPEDYIKGFPWHPHRGIETITYLIKGNMEHSDSMGNKGMILDGS
                     CQWMTAGSGVMHQEMPKATERMLGAQLWLNMPKENKMAPPNYCDIKAEMVPVIEEDGT
                     RVRVVAGDYKGKTSSVYGDYVKMLYLDVDMKAGAEWTLKTKEDETLFVYIIKGEGYFG
                     ENDSITESRRAVLFDSGDEFKIKAGDNGIRFFLYSAKPLKEPVAWGGPIVMNTREELR
                     QAFEELEKGTFIK"
     assembly_gap    1215..1314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1350..2378)
                     /locus_tag="PKK75_06990"
     CDS             complement(1350..2378)
                     /locus_tag="PKK75_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83382.1"
                     /translation="MLLFRIGPRHLFLRTKNINEVTNFLVTSLKGEIIDFWQGFEKAS
                     ENSTLCYITDIDHEKTYVEDAKKIVLINDASSPILCSIISSHISKLLQKVDMGPATIV
                     MRTAGDEQKLADRLKEIFGGREVDWIEGIGIGEKDDTMIAFTRKVLNEPVSDFLDTKL
                     LIAKPIREVQNKLRLEGLRLITQNLDDSQWYELRINIYDSYGNYYEHYERLMFVLSKL
                     EMGMVLGESWTKDFGVILYSVMTYQVRLFTFHTPIEIKKIMMALEFTVEGSRLVDLDL
                     YYKNKKVYWYEVNKNRGKGKTKADEAKVYREDLYKKLKQEDIKTLEAMEKSIENKHKS
                     QGDGGTVM"
     gene            complement(2395..3018)
                     /locus_tag="PKK75_06995"
     CDS             complement(2395..3018)
                     /locus_tag="PKK75_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ83383.1"
                     /translation="MKNIKDFYKNLAGRFNYPHIKKDMLNEIKGPVLIHISDTPSEIY
                     NAVFKIIDILKPQYIIHTGDLADNIKLEINRDRIKGYYFLVKQLVAGLERSDTKVYYL
                     LGNHDDYETVSRLTNKGAILEEGLLTIDNLSFAVGHYYKEYSLKTDFNLYGHSFEPGH
                     HVKDGTIGLNGVLNINVIDLSNKRVFHLNYPYSTNRLRGMENKRIGL"
     gene            3309..4271
                     /locus_tag="PKK75_07000"
     CDS             3309..4271
                     /locus_tag="PKK75_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNZ83384.1"
                     /translation="MRKILITGALGQIGSELTEEMRRIYGPENVVASNRRIKEGFDEL
                     IESGPFEVININDPGTLASAVDKHKINTIINLAAVLSAVGEKDPHQCWNINMNGLYNV
                     LEVAKEKNCMVYTPSSIAAFGPGTPKDKTPQDTIQRPTTMYGVTKVAGEILCDYYFKK
                     FGVDTRGVRFPGLISYKTLPGGGTTDYAVHIYYEALKNKKYSSFIQKGTYMDMMYMPD
                     ALNAIIDLMEANPDKLKHRNAFNVSAMSFEPEMLAASIRKYIPEFELDYDVDPVRQGI
                     ADSWPNSLDDTAAREEWGWSPKYDLDAMTKDMLENIRIKLNIPE"
     gene            complement(4427..5548)
                     /locus_tag="PKK75_07005"
     CDS             complement(4427..5548)
                     /locus_tag="PKK75_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNZ83385.1"
                     /translation="MNRGNYELAVRLRHELHEHPEISNNEVWTKKHLIDFLKDHTNLE
                     IVDRGQWFYAVYKGAEGRKNIAFRADIDALPIDEYLDIPHGSKIKGVSHKCGHDGHAA
                     ALAGFALEAERVGGDNNIYFLFQHAEETGDGAKEAAVFIRENNIDEIYAYHNMSGFPY
                     KAVVVKNGVNYCASKGMIIHMEGSPAHASMPEKGRNPSFALASIINAIPGLISPENYK
                     GMVLCTVIQVAAGERAFGTSASKGELLLTIRALYEEELGKLQKNIEDLAKEEAEKYGL
                     KITFNYVDSFPETYNHTVSIDKVRKAAVNKGYKLVEMKEAIRSSEDFGYYTKLTKGAI
                     FMIGNGEDYPSLHTNEYDFRDEIIETASDIFMGIIEINE"
BASE COUNT         1639 a         1245 c          898 g         1897 t
ORIGIN      
        1 cttaataaac atctttcacc attttttaat gtaatagttt tctcattgaa taccatatca
       61 atcacctgca atatattagt attattttat aagctccaca ctgtaggcat ttatttgggc
      121 aggatagctt tccataatca ttccatcata ggtaatttta actttactgc ctacagtaaa
      181 ttcagacaag tcttttaaaa cattgttttc tccgcggaca cgtacgacaa ttttatcgct
      241 tgaattaagc tcagatgagc cttcttccgg ttctaccaat ataaaattcc cattgttttc
      301 aagtactctt gcattgaagg atactttttc ttcatcgctg cctgtaatta atttatttac
      361 aacactaatt actattaatg ctaaaacaag caatactatc aaaaatatat actttttgcc
      421 catttatatc acctcaatta tatagacgca ctaagtaaag aaatgttccc taaaaaatga
      481 gactgaattt atcagcccca tgatttacta aaataattta ctttataaat gttccctttt
      541 caagttcttc aaatgcctgc ctaagctctt ccctggtgtt cattacaata ggacctcccc
      601 atgcaacagg ttctttcaga ggttttgccg aatacagaaa gaatcttatg ccgttatcgc
      661 cggctttaat tttaaattca tcaccggagt caaataaaac tgcccttctg ctttctgtaa
      721 tgctgtcatt ttctccaaaa taaccttcac ctttaattat gtaaacaaac aatgtttcat
      781 cttcttttgt ttttaatgtc cactcagcac cggctttcat atctacgtcg agataaagca
      841 tctttacata atcaccgtaa acagagcttg ttttaccctt gtagtcaccg gcgaccactc
      901 ttaccctggt cccgtcctct tctattaccg gcaccatttc tgcctttata tcacaatagt
      961 ttggcggtgc cattttgttt tctttcggca tattaagcca caactgggca cctagcattc
     1021 tctctgttgc cttgggcatt tcctgatgca tcacaccgct tccggcagtc atccactgac
     1081 agcttccatc caatatcatt cctttatttc ccatgctgtc gctatgttcc atattgccct
     1141 tgattaaata tgttattgtt tcaatccctc tatgggggtg ccaggggaat cctttgatat
     1201 aatcttccgg attgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntatcag
     1321 ccgtcatttt gccaatcttc gttccatttt cacatgacag tacccccgtc cccttgtgat
     1381 ttgtgtttgt tttctatgga cttttccatt gcctccaagg ttttaatatc ctcttgttta
     1441 agctttttat acaggtcctc cctgtagact ttagcttcat cagccttggt ttttcctttg
     1501 cctctattct tattgacttc gtaccaatac acctttttat tcttatagta gaggtccaag
     1561 tcaaccagcc tgctgccctc aaccgtaaat tccagtgcca tcattatttt ctttatttca
     1621 ataggtgtat gaaaagtaaa taatctaacc tggtaggtca tgactgaata aagaatcact
     1681 ccgaagtcct tagtccagct ttctcccagg accattccca tttccagctt tgacaataca
     1741 aacatcagcc tttcatagtg ttcgtaataa tttccatagg aatcatagat attgattcta
     1801 agctcatacc actggctgtc gtccaaattt tgtgtgatca gcctgaggcc ctccaatcgc
     1861 agcttgtttt gaacttccct tattggcttt gcaattaaaa gcttagtatc cagaaaatca
     1921 gaaaccggtt cgttaagtac ctttctggta aaggcaatca tggtgtcatc cttctcgccg
     1981 attccgatgc cctctatcca atcaacttcc ctgcctccaa aaatctcctt tagcctgtct
     2041 gccagctttt gttcatcacc ggctgtacgc attacaattg ttgccggacc catgtcaacc
     2101 ttttgcagca gtttagatat gtgactgctg atgattgaac acagtatagg cgaagaagca
     2161 tcattgataa gaacaatttt ctttgcatcc tctacatagg tcttttcatg gtcaatgtca
     2221 gtaatatagc aaagggtgct gttttccgat gccttttcga acccctgcca aaaatcaata
     2281 atttccccct taagactcgt caccaaaaag tttgtaacct cattgatgtt ttttgttctt
     2341 aaaaacaaat gccttggtcc aatcctgaat aaaagcattt ccttccccct ttctttataa
     2401 acctattctt ttattttcca tacctcttaa acgatttgtt gaataaggat aatttaaatg
     2461 aaataccctt ttatttgaca aatcgattac gtttatattt aatacaccat ttaaaccgat
     2521 tgtaccatct ttcacgtgat gaccgggttc aaagctgtga ccataaagat taaaatccgt
     2581 cttaagcgaa tattccttgt agtaatgccc cactgcaaag cttaaattat ctattgtcaa
     2641 aagtccttct tctaaaattg cacctttatt tgtcaaccgg cttacagttt cataatcatc
     2701 atggttgccc agcaaatagt aaacctttgt gtcacttctc tccagacctg ccacaagctg
     2761 ttttacaagg aagtaatagc cctttattct gtccctgtta atctccagct tgatattatc
     2821 tgccaaatca cctgtatgta taatatattg aggtttcaga atatcaatta ttttaaaaac
     2881 agcattgtaa atttccgaag gggtatcgct tatatgaatc aagacaggcc cttttatttc
     2941 attgagcata tcctttttaa tatggggata attaaatctg cctgccaggt tcttataaaa
     3001 atccttaatg tttttcatat atcctgaatc ctccttcctt ttaataaaac caacagtttt
     3061 gcaattaaat ttttcaatat ataattgtct tccatttatg tactatatta tatataattc
     3121 ctaatactaa agcaataatt tgaaattttt atttatattt tataaataat atggtattat
     3181 atatttaaac attatatgac cataaccctg acaataaagg gcgggttata catagaaaag
     3241 acaaatccat ggttactatg tattgtgtat aagtcatgga aacacatagc aaaatacggg
     3301 aggtaaaatt gagaaaaata ctaattaccg gagcattggg acaaataggc tccgaattaa
     3361 cagaagaaat gcgaagaatc tacggtccgg aaaatgtagt agcaagcaac agaagaatta
     3421 aggaaggatt tgatgaactt attgaatcag gtccgtttga agtaataaat atcaatgatc
     3481 caggaacatt ggcttcagca gtggataaac ataaaataaa caccattata aacctcgctg
     3541 cggttttgtc tgccgtcgga gaaaaagatc cccaccagtg ctggaatatt aatatgaacg
     3601 gtttgtacaa cgtcctggaa gttgccaagg aaaaaaactg catggtgtac acaccatctt
     3661 ccattgcagc cttcggcccc ggaactccaa aggacaaaac tccccaggat acaattcaaa
     3721 gacctacaac catgtacgga gtaacaaagg ttgccggaga aatattatgt gactattact
     3781 ttaagaagtt cggtgttgac accaggggag tccgtttccc gggacttatt tcatacaaga
     3841 cgctgccggg aggaggaaca accgactatg cagtccatat ctactatgag gctctgaaga
     3901 ataaaaaata ctcaagtttc atccaaaaag gaacctatat ggatatgatg tatatgccgg
     3961 atgcattaaa tgcaatcatt gacttaatgg aagcaaatcc ggacaaacta aaacaccgca
     4021 atgcctttaa cgtttcagcc atgagctttg aacctgaaat gctggctgca tccataagaa
     4081 aatacatacc tgaatttgaa cttgactatg atgttgaccc cgtaagacag ggaatagctg
     4141 attcatggcc taactcgctg gatgatacag ctgccagaga agaatggggc tggagtccaa
     4201 aatatgacct ggatgccatg acaaaagata tgcttgagaa tataagaatc aagttaaata
     4261 taccggaata acaaataaat taattgggga cattccttct attcatttct cagagaaggt
     4321 ttgtccccta actttgttca gtgtgtcgtc ttagttcatt atgtctgtct tcgttcattg
     4381 tgtccaatga tatttatatg agtccagtgt tttctgttac atatcttcat tcatttattt
     4441 caattattcc cataaaaatg tctgaagcgg tttctataat ttcatccctg aaatcatatt
     4501 cgttagtgtg cagagaaggg taatcctctc catttccaat cataaagatg gcgcccttgg
     4561 ttaatttggt gtaatatcca aaatcctctg aacttcttat tgcttccttc atttctacaa
     4621 gtttgtatcc cttgtttact gcagctttcc ttactttgtc aatacttact gtgtgattgt
     4681 atgtttcagg gaaagaatca acataattaa aggttatctt cagcccatat ttttctgctt
     4741 cctcttttgc caggtcttca atattcttct gcagcttacc cagctcttcc tcatacaagg
     4801 cccgaattgt cagcagcagt tcccccttgc ttgcactggt gccaaaggct ctctcccctg
     4861 cagccacttg aattacagtg caaagaacca ttcccttgta gttttcaggc gaaattagtc
     4921 cgggtatggc atttattata cttgcaagag caaaggatgg attccttccc ttctccggca
     4981 tgctggcatg ggcaggactg ccttccatat gtattatcat accctttgaa gcacagtagt
     5041 taactccgtt tttcaccaca acagctttat aggggaaacc gctcatattg tgataagcat
     5101 aaatttcatc aatattgttt tctcttataa atacagctgc ttcttttgct ccgtctccgg
     5161 tttcttcagc atgctgaaat aaaaagtata tattgttgtc ccctccaacc ctttctgctt
     5221 caagggcaaa tcctgctaat gccgcagcgt gcccgtcatg tccgcattta tgggaaacgc
     5281 ctttaatttt agacccgtgt ggaatatcca gatattcatc gattgggagt gcatcaatat
     5341 ccgcacgaaa agctatgttt ttccttccct cagccccttt gtatacagca taaaaccact
     5401 gtcctctgtc aacaatttct aagtttgtgt gatcctttaa aaaatctatc aaatgttttt
     5461 ttgtccatac ttcattgttt gatatttcag gatgctcatg cagttcatgg cgcagtctta
     5521 ctgccaactc ataatttccc ctgttcataa tccctcctgt gaaatttcgt caaattctgt
     5581 tatatcgggg gcagaccctg aaattcagga gaattctcaa ggacccttaa taaaacttct
     5641 caaatcaata atatcacttt ttataactgt tatcaattat gaacatttat ttataaccct
     5701 ttatcttcaa ctttgtattg caaaaatgcc attaaaatga taaattatta ttgggaaaat
     5761 attttcaaac ataagaaag
//
LOCUS       DAOPUT010000087         5683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_14160_length_5683_cov_19.639837, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000087 DAOPUT010000000
VERSION     DAOPUT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14160_length_5683_cov_19.639837"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..877
                     /gene="panB"
                     /locus_tag="PKK75_07010"
     CDS             11..877
                     /gene="panB"
                     /locus_tag="PKK75_07010"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNZ83386.1"
                     /translation="MAKKKTILDLYKMKEKGEKAVWVTAYDSCFASYAEKAGMDMILV
                     GDSMGMIVYGYQGTTPVTMDMCINHCQAVRRGAPNTYVIGDMPFGSYHESVEDSVHNA
                     VRFIKEADVDAIKLEGGIAVEDKIKAIAQAGIVVFGHVGLTPQSSAAMGGFKAQGRTT
                     ESARAVINDALAVYKAGAKFVLLEGVPEEVCAFVHKILPIPVYGIGAGADTDGQVLVI
                     GDALGMYEEFIPKFAKKYADIAGAATQGFAQYVKEVKEGTFPAPEHKYKISGDVSEFD
                     RLFKEMEEEIRL"
     gene            939..2183
                     /locus_tag="PKK75_07015"
     CDS             939..2183
                     /locus_tag="PKK75_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNZ83387.1"
                     /translation="MKNKITLLLCILLVAAMVLGGCASPSGDLMKGILSQNKNRVPES
                     MNTELNQAVLNFAWNLFKESSENQGNIMISAPSAYFALAMTLNGANNETRTGMLKALS
                     ADNIDLEELNTGLNDWMTLMAENRTAKFSITNSIWLREGYNVNKDFLQTNADYYSAYI
                     KSLDFGEASAPDEINSWVKEATNGKIDKIVDKISDDLIMYLINAIYFKGDWKEQFSAN
                     KTFEMEFNAPTGKVKADFMNRRGDIDYLKLNDITGVVLPYEDEQFAFVGLLPAQGQTP
                     GDLINELSALDLTRLLAGKEVKNIELSLPKFETSYEDSLKNELSKLGMEIAFQPYNAD
                     FSLMRQENDLYIGDVKQKTYIKVDEKGTEAAAVTSVEMELTSMPVELPKVVFDRPFVY
                     GIIDVETGIPLFMGIMENPAIK"
     gene            complement(2236..3162)
                     /locus_tag="PKK75_07020"
     CDS             complement(2236..3162)
                     /locus_tag="PKK75_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNZ83388.1"
                     /translation="MKKKDILLAALIVLIWGNNFTVIKLGLDGVPSMLLVSLRYILTS
                     FPAVFFIKKPQTELKYIVLYGLTVGVGQFSCLFYAMEIGMPAGIASIVLQIQAFISPV
                     LAMIFLKEKIKRKQIIGFVISAAGLVLIAMASTGDSINKVPALAFILMLAAPTFWAVS
                     NMVARIASDKAAAKGEKLNMVSMVVWSGLVPPIPLMAMALMLDTPETILNILTNLNGT
                     SIFAVLYIAFAATLMGYGAWSVLIAKYPMSIVAPITLMVPIVALLSARIVLSEQLTQL
                     QWVGLIVILAGLFTANINPGIIKNIFKSKNAI"
     gene            3352..3573
                     /locus_tag="PKK75_07025"
     CDS             3352..3573
                     /locus_tag="PKK75_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HNZ83389.1"
                     /translation="MAEIKFEITEELAVLSESAKGWTKELNMVSWNDREPKFDIREWS
                     PDHERMGKGVTLNREEMKIIKYILNEINL"
     gene            complement(3687..4577)
                     /locus_tag="PKK75_07030"
     CDS             complement(3687..4577)
                     /locus_tag="PKK75_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNZ83390.1"
                     /translation="MQETKKKSKLGFLIKLIIFIVLVVSITNTAYIVRENEYAYVTRF
                     SKFVKIQDTAGLHFKLPLLDKIEKIPQYRMMYDIPPSEVLTGDKKTLVVDNFTVWQID
                     DPYAFMRTVSRISEMENRIDAVVYNAVKNTLGTMNQTAIINSDNSSIDDVNDKITELV
                     NAQLKNYGVSTIAVEIKRLDLPKDNESAVYNRMISERTQMAESFRAEGNLEASKVVNE
                     TDKEVGILLSKASAAAEELKGQGEGEYMKIIAEAYSTEDRMNYYEFIRSLEALKVTMK
                     GEKTLILPADSFIVRVLNGY"
     gene            complement(4577..5620)
                     /gene="hflK"
                     /locus_tag="PKK75_07035"
     CDS             complement(4577..5620)
                     /gene="hflK"
                     /locus_tag="PKK75_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNZ83391.1"
                     /translation="MNLKNIIINLLSSIKKPRDFQDQDLQFENKPPVRRGKLKFLLIL
                     PVLLIAVAVIISCQYTVEETEQAVVTTLGKVTSVESAGLHFKLPYPIQGVTKVAVNKT
                     QKLQIGYSSESESDSYLYTSVADEAKMITGDFNIVNIDFFIEWKISDPVKFLYNSDEP
                     AQILKMVSQSSARSIIGSKDVDGVLTTEKSIIQAEIKEKIVNKLENYDLGIQLLDVKI
                     QDSQPPTQEVIQAFKEVETAKQEKETRINEALAYRNKTLPAAESSADKLIREAESYRE
                     SKINEAKGDVARFNAMYTEYAKNKDITRTRMYLETIEEILPGVKVYIDSSEGGVQKLL
                     PLESFNTENGGEN"
BASE COUNT         1751 a         1046 c         1135 g         1751 t
ORIGIN      
        1 ggaggttatt atggcaaaga aaaaaacaat tttagactta tacaagatga aggaaaaggg
       61 tgaaaaggca gtatgggtta cggcttatga cagctgtttc gcatcatatg ccgaaaaagc
      121 aggaatggat atgatattgg taggagattc catggggatg atagtctacg gctaccaggg
      181 aactacaccc gtaaccatgg atatgtgcat aaatcactgc caggcagtga gaagaggcgc
      241 cccaaataca tatgtaatcg gtgatatgcc ctttggctct tatcatgaaa gtgttgaaga
      301 ttcagtgcat aatgctgttc gcttcattaa ggaagcagat gtggatgcaa taaaattaga
      361 gggcgggatt gctgtagagg ataaaatcaa agcaatcgct caagcaggca tagttgtatt
      421 cggacacgta gggctgacac ctcaaagctc agctgccatg ggcggcttta aagcccaggg
      481 tcgtacaaca gaaagcgccc gcgctgtaat aaatgatgcc cttgctgtat ataaggcagg
      541 agcaaaattc gttttgctgg aaggagtgcc ggaagaagta tgcgcctttg ttcataaaat
      601 tctgcccatt ccggtatacg gcataggagc aggagctgat actgacggcc aagtattggt
      661 tatcggagac gctttaggaa tgtatgaaga gttcataccg aaatttgcaa agaagtatgc
      721 tgacatagca ggtgctgcaa cacaagggtt tgcacaatat gtgaaggaag taaaagaagg
      781 tacattccct gctcctgagc ataagtataa aatttcaggt gatgtttcag agtttgacag
      841 attgtttaag gaaatggaag aagaaatcag gctgtaatgg attcaaagga acattattca
      901 ggtaaaatcc gtctaagctt tacaaaggag gtttttacat gaaaaataaa attactttac
      961 tattatgcat actgcttgtt gcagctatgg ttttaggagg ctgtgcatct ccatcgggag
     1021 atttgatgaa ggggatatta tcacaaaata agaacagggt gcctgagtca atgaatactg
     1081 aattaaatca agctgtattg aattttgcct ggaacctgtt taaagaaagc agcgaaaacc
     1141 aggggaatat tatgatatct gcaccctctg catactttgc actggcgatg acattaaacg
     1201 gagcaaataa tgaaaccagg acagggatgc tgaaggcact gagtgctgat aatatagact
     1261 tggaagagct gaatacaggc cttaatgatt ggatgacact gatggcagag aacaggactg
     1321 caaaattttc aataaccaat tcaatttggc tgagagaagg ttacaatgta aataaggatt
     1381 ttcttcaaac aaatgcagat tattattcag cttacataaa gagcctggat tttggcgagg
     1441 cttcagcgcc ggatgagata aacagctggg tgaaggaagc caccaacggg aaaatagata
     1501 aaatagttga taaaataagc gatgatttga tcatgtattt aatcaacgca atttatttca
     1561 agggagactg gaaggaacaa ttttcagcca ataaaacctt tgagatggaa tttaatgcac
     1621 caacgggcaa ggtaaaagcg gacttcatga accgaagagg cgatattgac tatctgaagt
     1681 taaatgacat aacaggagta gttcttcctt acgaagatga acaatttgct tttgtgggct
     1741 tgctgcctgc tcagggtcag acaccaggag accttataaa tgagctgtca gccttggatt
     1801 taaccaggtt attggcaggt aaggaagtta aaaacatcga gctttcactt cctaagtttg
     1861 aaacctccta tgaagacagc ttgaaaaacg agctgtcaaa attgggaatg gaaatagcat
     1921 tccaacccta caatgctgat ttttcactta tgagacaaga aaatgatttg tacatagggg
     1981 atgtaaaaca aaaaacatat ataaaggtcg atgaaaaggg aacggaagct gcagcggtaa
     2041 catcagttga aatggaattg acctcaatgc ctgtagagct gccgaaagtc gtattcgaca
     2101 gaccctttgt ctacggcatt atcgatgttg aaacaggaat ccccctattt atgggaataa
     2161 tggaaaatcc cgcaataaaa taagtgtaat cacaattaaa ggctgttgag atataggttg
     2221 tctcagcagc cttttttaga ttgcattttt cgacttaaaa atattcttaa taataccggg
     2281 attaatattt gcagtaaaaa gtcctgctaa aatgacgatt aagcccaccc actgtaattg
     2341 tgtcagctgt tctgaaagaa ctattcttgc tgacaagagt gctactatgg gaaccataag
     2401 ggtaattgga gcaactatgc tcataggata ttttgctatt aatacactcc aagcaccata
     2461 tcccattaat gtagctgcaa atgctatata taacactgcg aatattgaag tgccgttcaa
     2521 gtttgtcagt atattaagaa tggtttcagg tgtgtccagc ataagtgcca tagccatcaa
     2581 tggaatcggg ggaacaaggc ctgaccatac aaccatgctt accatattca gtttttcacc
     2641 ctttgcagca gctttgtcgg aagcgattct cgcaaccata tttgatacag cccagaatgt
     2701 aggtgcagct aacataagta taaaggctaa agcaggaacc ttatttatac tgtctccggt
     2761 ggaggccata gctataagta ccaaacctgc agctgatatt acgaatccaa ttatttgctt
     2821 cctttttatc ttttccttta aaaatatcat tgccagaacc ggtgatataa aagcctggat
     2881 ttgaaggaca atcgaagcta tgcctgcagg cattccaatt tccattgcat aaaacaagca
     2941 ggaaaattgt ccaacgccta cagtaagtcc atataataca atatatttta attccgtttg
     3001 gggctttttt ataaaaaata ctgcaggaaa tgatgtaaga atgtatcgta atgaaaccag
     3061 cagcatagat ggaactccgt ctaatcccag ttttataact gtaaaattat taccccatat
     3121 taatactatc aaggctgcca gcaatatgtc ttttttcttc attttcttgt ttctcctaaa
     3181 gtgatatttt aattatagca cagtttaaaa cactttgata tattatttaa tctgcggtat
     3241 aatataataa atcaaattgt gacaacttct tgggagtgaa aggaaacatc aaatattgtt
     3301 gaaatattaa cggatgtaaa attagaataa aaaaactatg gaggataaaa gatggctgaa
     3361 ataaaatttg aaataaccga agaattagcc gtgctttctg aatcggcaaa gggctggaca
     3421 aaggaactga acatggtaag ctggaatgac agagaaccta aatttgacat aagggaatgg
     3481 tccccggacc atgaaagaat gggaaaaggg gtaaccctga acagagaaga aatgaaaata
     3541 atcaagtata tactaaatga aataaacctg taatcaatat agaaacagtt aatctgccag
     3601 tttataacat gcatatgata aaaagctcat tcgaaataga atgagctttt aagtttctag
     3661 tcctactccg gagtgcctgt tttgcattaa tagccgttca gtactctgac aataaagctg
     3721 tcagcaggca ggatcagtgt tttttcaccc ttcatcgtga ccttgagggc ttccagactt
     3781 ctgatgaatt cgtaataatt cattctgtct tccgtagaat aagcttcggc aataattttc
     3841 atatattcgc cttcaccctg cccctttaat tcttctgcag cggcagatgc tttagataaa
     3901 agtattccaa cttccttatc tgtttcatta accaccttgc ttgcttccaa attaccttct
     3961 gccctgaagc tttccgccat ttgtgttctt tctgaaatca ttctgttgta tacggccgat
     4021 tcattgtctt ttggcaggtc taatctttta atttcaactg caatagtgga tacaccgtag
     4081 tttttaagct gggcattgac cagttcggtt attttatcat taacgtcgtc aatggagctg
     4141 ttgtctgaat tgataatggc agtttggttc attgttccca gtgtgttttt aactgcattg
     4201 tatacaaccg catcaatacg gttctccatt tcagatattc ttgatactgt cctcatgaat
     4261 gcataggggt catctatctg ccatacagta aaattgtcga ctaccaatgt ttttttgtcc
     4321 cctgtcaata cttctgacgg aggtatatca tacatcatcc tgtactgagg tattttttct
     4381 attttatcca atagggggag cttgaaatgc agtcctgcag tatcctggat ttttacaaat
     4441 tttgaaaatc tggttacata ggcatattca ttttctctta ctatataggc tgtgttggta
     4501 attgaaacaa ccaatacaat gaatataatt aattttataa gaaaccctaa tttgcttttc
     4561 tttttagttt cctgcattag ttttcacccc cattttctgt attaaaactt tccaaaggca
     4621 gtaatttttg cactccgcct tctgatgaat caatatacac ctttacgccg ggcagaattt
     4681 cttctatggt ttccaaatac attctggttc tggttatatc cttattcttg gcatattctg
     4741 tatacattgc attgaatctt gccacatcgc cttttgcttc attgatttta gattccctgt
     4801 aggattcggc ttccctgatt aatttatctg cgctgctttc agctgcaggc agggttttat
     4861 tcctgtaagc caatgcttca tttatccttg tttccttttc ctgctttgct gtttcaactt
     4921 ctttaaaggc ctggattacc tcttgagttg gaggctgact gtcttgaatt ttgacatcta
     4981 aaagctgaat tcccaaatca tagttttcca gtttgttgac tattttttcc ttaatctccg
     5041 cttggattat tgatttttca gtagttaaaa caccatctac atcttttgaa ccgattatgc
     5101 ttcttgctga tgactgtgac accatcttta aaatctgtgc cggttcatct gaattataca
     5161 aaaactttac ggggtctgaa attttccatt cgataaagaa gtctatattt acaatgttga
     5221 agtctccggt tatcattttc gcttcatctg ctactgatgt gtataaataa ctgtcagact
     5281 cactttcgct tgagtagcct atttgtaatt tttgtgtttt atttacagct acctttgtta
     5341 caccctgtat ggggtagggc agtttaaaat gcaatcctgc cgattcaacg cttgttacct
     5401 tcccaagggt tgtcaccact gcctgttctg tttcctccac tgtgtactgg catgagatta
     5461 ttacagcaac tgcaataagc agtacaggca ggattaacaa aaacttcaat tttccccgcc
     5521 ttaccggagg tttattttca aactgcaggt cctggtcttg aaaatcacgg ggtttcttaa
     5581 tcgaacttaa caaattaatg attatatttt taagattcat ggctgtctcc tttattatta
     5641 caataatata aacattattg ccctataatt atatcataaa agc
//
LOCUS       DAOPUT010000088         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_14191_length_5673_cov_19.785867, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000088 DAOPUT010000000
VERSION     DAOPUT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14191_length_5673_cov_19.785867"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2598)
                     /locus_tag="PKK75_07040"
     CDS             complement(<1..2598)
                     /locus_tag="PKK75_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNZ83392.1"
                     /translation="MLIQQQIIIRRKNNLEWYNKGKNEVLKELNTNLSAGLSEKEAEK
                     RIEVYGRNELEEQAKKSFFRKFIAQFADFLIIILLLAAGVSAFVGEKEDAIIILAIVV
                     INAALGIYQEGKAEKSVEALQKLSAPNAKVVRDSNLIVIPAAEIVPGDVAVLEAGDIV
                     PADLRLFESSNLKIEEASLTGESVPVEKDAKAKIVGRVGIGDRHNMGFSSTIVTYGRG
                     RGVVTATGHNTEIGNIAVKIQTYGEEDTPLQIKLNQLGKVLGTLTIAICVIVFIVGML
                     QGRQPLNMLLTSISLAVAAIPEGLPAIVTIVLAIGMNRMAGKNAIVKKLLAVETLGAT
                     SVICSDKTGTLTQNEMTVVKAYVDDKILDVEGIGYEPKGDIKLNGRNVSISSLPDLRN
                     LVSIGTLSNDAQLDNSTGIYKIIGDPTEGAIVAFAGKLGQTSDQLNMIYPRIAELPFD
                     SGRKMMTTFHSNYIEGKVVSFTKGAPDIVINKCSKIALNGRIVDFNKELKDKVLSVNT
                     KFARSALRVLSAAYKVWDRLPDHPTFEKVEDDMIFVGLVGMIDPPRPEVKESIAYCRQ
                     AGIETVMITGDYKETAYAIARELGMVEHEGQAIMGEDLDEYSDEKLREVVKKTKVYAR
                     VSPDHKVRIVTALKENGHITSMTGDGVNDALALKKADIGVAMGITGTDVAKNTAEVIL
                     TDDNFATIVNAVEEGRIIFSNIKKFVFFLLSCNIGEILLVFISILLGWEVPLLPIQLL
                     WLNLVTDSFPAMALGVENAEPGIMKQPPRSTKEAILDKEMLGGIAFQAVAISFASLLS
                     YYWAVKMYGTATGLIHARSVVFTTLILSELLRAFSSRSQNYTLFKIGFFTNIRMVQAV
                     FLSFILTFAV"
     gene            complement(2817..>3452)
                     /locus_tag="PKK75_07045"
     CDS             complement(2817..>3452)
                     /locus_tag="PKK75_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ83393.1"
                     /translation="YPRPYRDKVRTFCQNPGGYVSQENYDNNLAVVNGHSYKDRKTSN
                     TNLAILSSHNFSDPFNEPIKYGAKVAELLNMLGDGKILVQRYGDILDGKRTWQHELSR
                     ANVKPTLPDAVAGDITSAMPYRTMTNILNFIEALNIVVPGFAGTETLLYGPEVKFYSN
                     KVELSAEFETNIKKLYCLGDASGWTRGLMMSSLMGVRMGQILGKEDTQNKV"
     assembly_gap    3453..3552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3553..3968)
                     /locus_tag="PKK75_07050"
     CDS             complement(<3553..3968)
                     /locus_tag="PKK75_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ83394.1"
                     /translation="MKYDLIIVGAGPSGIFTALELTRENPNKKILIIEQGKPIEKRNC
                     PKDKTKKCMNCKPHCNITTGFAGAGAFSDGKLSLSPEVGGDLPELIGYDFTQEMIEYT
                     DAIYLEFGADSRVEGVEAKEEIKEIRRKAIEAGLKLV"
     gene            complement(3998..5173)
                     /locus_tag="PKK75_07055"
     CDS             complement(3998..5173)
                     /locus_tag="PKK75_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNZ83395.1"
                     /translation="MKKKKYLIAAGVIIIVIIASLLLGRQTDELETSKVKIGSIKNTI
                     EETGIVFSKRVNTFYSDMSQKVDTLIVSAGDKVKKGDVILVYENNYDLEIERANKQIE
                     VITASYNESVKGADFQEISNVKLNISTIEANLGFARSNLEKIEALYENNAVSRVEYDE
                     AVNNVAVLENQLQEANNNYSLMIQGVSSNVKKQYEAQIDEVVILKQILEKNKEQSSIK
                     AEFDGVITELNVYQGSMTQAGVPVVEIQDDLSLGIYVEVLAEDAMEVIKGMDFNIMDD
                     DEVIKGLLISRIYPKAQSKISDLGVEQKRVRIEADLDDTSYKLGSEVDVEIVLMEKND
                     ALLVDKDSVYEKDKKKYVTVIKGNNETETEITTGLEDDVNVEVLSGLNENDVVLLGF"
     gene            complement(5187..>5673)
                     /locus_tag="PKK75_07060"
     CDS             complement(5187..>5673)
                     /locus_tag="PKK75_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83396.1"
                     /translation="KLDDIINIMSIQSQEEMQGVFEEFTGLIMVFMGFMVIFSGMLGF
                     VIVYSMTLMSINERTLEFSSLRVMGFTKIEIFRMLVKENMVMSVIGIIAGIPLGNWLV
                     DYIGIMFNTDIYTLIGIVTTKDIITAIILTVIFIVSAQLMTYVKIKKLDFMQALKSRI
                     T"
BASE COUNT         1516 a         1314 c          837 g         1906 t
ORIGIN      
        1 aaccgcaaag gttaaaataa aggataaaaa cactgcctga accattctta tattggtgaa
       61 aaatcctatt ttgaacaagg tataattttg agaccttgaa gaaaatgccc ttaaaagctc
      121 tgacaatatc aatgttgtaa atactacaga ccgggcatga atcaaaccgg ttgccgtgcc
      181 gtacattttc accgcccaat aatatgacaa cagggatgca aatgatatgg caacagcctg
      241 aaaggcaatt ccgccaagca tttccttatc aagtattgct tcctttgtgc ttctgggagg
      301 ctgtttcata atgcccggct ctgcattttc aactcccaat gccattgccg gaaaactgtc
      361 cgtaacaaga ttcagccaca aaagctgtat tggaagaaga ggaacctccc agcccagcaa
      421 aatacttatg aataccagta aaatttcacc gatattgcag cttaataaaa agaatacaaa
      481 tttctttata tttgaaaaaa ttattcttcc ttcttctacg gcatttacta tagttgcaaa
      541 attatcgtcc gtaagtataa cttcagctgt gtttttagcc acatccgttc ctgtaattcc
      601 cattgcaacc ccaatatctg cctttttaag ggcaagagca tcattgactc catcccccgt
      661 cattgaagta atatgcccgt tttcctttaa tgcagttact atcctgactt tatggtcggg
      721 tgataccctg gcataaacct tggttttctt tacaacttcc cttagctttt catcagagta
      781 ttcatccagg tcctctccca ttattgcttg accttcatgt tcaaccattc ccagttccct
      841 tgcaattgca taggctgttt ccttgtaatc tcctgtaatc attacagttt caatacctgc
      901 ttgcctgcaa taagcaatgg actcttttac ttcaggtctg ggtggatcaa tcattcctac
      961 taatcctaca aatatcatgt catcttcaac cttctcaaat gttggatggt ccggcagtct
     1021 gtcccatacc ttgtaggcag ccgataaaac tcttaatgct gatcgtgcga acttggtatt
     1081 tacagacagt actttgtcct ttaactcttt gttaaaatca acaattcttc cgttaagtgc
     1141 aattttactg catttattta taacaatatc tggagcaccc tttgtaaatg aaacaacttt
     1201 tccttcaata taattggagt ggaaggttgt catcatcttt cttcctgaat caaaggggag
     1261 ttctgctatt ctcggatata tcatgttgag ctggtctgat gtttgtccta atttgccggc
     1321 aaaggcaact attgctcctt ccgtaggatc gccgataatt ttatatatgc ctgtagaatt
     1381 atccaattgt gcatcatttg ataatgtgcc aatagaaact agatttctta aatcaggaag
     1441 agaacttatg cttacattcc ttccgttaag ctttatatct ccttttggct cgtatcctat
     1501 accctcaaca tccaaaattt tgtcatcaac atatgctttt acaacggtca tttcattttg
     1561 tgtcaaggtc cctgttttat ccgaacaaat aaccgacgtg gcacccaggg tttctactgc
     1621 caaaagtttt tttacaatgg catttttccc tgccattctg ttcattccaa tagccagaac
     1681 aattgtaaca attgccggca aaccctccgg tattgctgcc actgccagac ttatggaagt
     1741 caaaagcata tttaagggtt gtcttccttg aagcatgcct actataaaaa ctattacaca
     1801 aatggctatt gtaagtgttc caagaacctt ccccagctgg tttaatttaa tctgaagagg
     1861 tgtgtcttct tccccgtagg tttgtatttt aacagcaata ttaccgattt ccgtattgtg
     1921 tcccgttgct gtaaccactc ccctgcctct gccgtaggtt acaattgtac ttgagaaccc
     1981 catattatgc ctgtccccta ttccaactct gccgacaatt tttgcttttg catccttctc
     2041 aacgggaact gattctcctg taagagatgc ttcttcaatc tttaaattag aactttcgaa
     2101 aagccttaaa tcagcaggaa ctatatctcc tgcctccaag acagccacat ctccgggaac
     2161 aatctctgca gcaggaatta caattaaatt gctgtccctt acaaccttgg cattgggggc
     2221 actcagtttt tgcaaggctt ctaccgattt ttccgccttt ccttcctgat aaattccaag
     2281 ggcggcattt attactacta ttgcaagaat tattatcgca tcttcctttt cgcctacaaa
     2341 tgcagatacc cctgctgcta aaagcagaat aattatcagg aaatctgcaa attgagcgat
     2401 aaactttcta aagaagctct tttttgcctg ctcttccaat tcatttctgc cgtagacctc
     2461 aattcttttt tcggcttcct tttcacttaa tcccgcactt aaatttgtgt ttagttcctt
     2521 taaaacttca tttttgcctt tgttatacca ttccaaatta tttttcctcc tgataataat
     2581 ttgttgttga atcaacatta gtataatata ttggcaattt ctttatttat tacaaaaatt
     2641 tgtacaaccc ttaacttatc aaaataagaa aaaataccct ccaaacttaa aagagagtat
     2701 tactaatttg gggacattcc tcgtactgca aagactccta aattggggac attcctccga
     2761 ccttaaagac ttaccctata gtagaggtgt gtccccatac ctatttaggt gtatcctcat
     2821 accttgtttt gtgtgtcctc tttccctaat atctgtccca tccggacacc catcaaggaa
     2881 gacatcatca atcctcttgt ccagccgctt gcatcaccca aacagtacaa ttttttgata
     2941 tttgtttcaa attcagcact aagttcaacc ttgttgctgt aaaatttcac ttcaggtccg
     3001 taaagtagtg tttccgttcc ggcaaacccc ggcaccacaa tatttaatgc ctcaataaaa
     3061 tttaaaatat tagtcatagt cctgtagggc atggctgagg ttatatcccc tgcaactgca
     3121 tccggcagtg tgggtttgac atttgccctg gacaattcat gctgccatgt tcttttgccg
     3181 tccaagatat caccatatct ttgaaccaag attttcccgt ctccaagcat gtttaaaagc
     3241 tctgcaacct tggctccata ctttatgggc tcattaaaag gatcactgaa attatgggag
     3301 cttaaaattg ctaaattagt attgctggtt ttcctgtcct tgtaggagtg gccgttaaca
     3361 actgcaagat tgttgtcgta attttcctgg cttacataac ctcccggatt ctgacagaag
     3421 gttcttacct tgtccctata gggccttggg tannnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnacaagctt tagaccggct tcaatggcct ttcttcttat ttccttgatt
     3601 tcttccttgg cttcaacgcc ttcaaccctg ctgtcagcac caaattcaag ataaatagca
     3661 tctgtatatt ctatcatctc ttgtgtaaag tcatatccta tcaattcagg caaatctccc
     3721 cccacctccg ggcttaagga cagcttgccg tcagaaaatg ctcccgcccc tgcgaatccg
     3781 gttgtaatat tgcagtgagg cttgcagttc atgcattttt tggttttatc cttagggcaa
     3841 tttctctttt caataggctt cccctgttct attatcaata tttttttgtt agggttctct
     3901 ctggtcagct ccaaggctgt aaatattcct gaaggacctg cccccactat aatcaaatcg
     3961 tatttcataa ttttctcctt tttctttggg acatttccta gaaccctaat aaaactacat
     4021 cattctcatt aagtcccgac aaaacttcca catttacgtc atcctcaagg ccggttgtta
     4081 tttcggtttc tgtttcatta tttcccttta ttacagtcac atattttttc ttgtcctttt
     4141 cataaacaga atccttgtca accaataagg catcattctt ttccataaga acaatttcca
     4201 catcaacctc cgagcccagc ttgtaagatg tatcatctaa atccgcctca attcttacgc
     4261 gtttttgttc aacgcccagg tcggaaattt ttgattgagc ctttggataa attctgctta
     4321 tcagcaatcc ctttataact tcatcatcat ccataatatt aaaatccatt cccttaatta
     4381 cttccatagc atcctcagcc agtacttcca catagatgcc aaggctaagg tcatcctgaa
     4441 tttcaacaac aggaacccct gcctgtgtca tgctcccctg ataaacattc agctctgtaa
     4501 ttactccgtc aaattcagct tttattgaag actgctcctt gttcttttcc aatatttgtt
     4561 ttagaatcac cacttcatca atttgagctt cgtactgctt ttttacattg gatgaaacac
     4621 cctgtatcat caagctgtag ttgttgtttg cttcctgaag ctggttttca aggactgcaa
     4681 cattattcac tgcttcatca tattccaccc ggctgacggc attattttca tacaaagctt
     4741 caattttttc aagattgctt cttgcaaaac caagattagc ctctatagtg cttatattta
     4801 gctttacatt ggaaatctcc tgaaaatccg ccccttttac cgattcatta taagatgctg
     4861 ttattacttc aatttgtttg tttgctcttt caatctccaa atcatagtta ttttcataaa
     4921 ctaaaattac gtcacccttt tttaccttgt ctcctgcaga cacaataaga gtatcgactt
     4981 tttggctcat atcagaataa aaggtattaa ctctttttga gaacactatc cctgtttcct
     5041 caatcgtatt cttgatgctc ccgattttaa ccttcgatgt ctcaagctca tcagtttgcc
     5101 ttcctaaaag taatgaagct ataatgacta ttattattac tcctgctgct ataagatatt
     5161 ttttcttctt catttttacc tccgctctaa gtaatcctgc tctttaatgc ctgcataaaa
     5221 tctaactttt taatcttcac gtaggtcatc agctgggctg aaactataaa tattacagtt
     5281 aaaataattg cggtaattat atcctttgtt gtgacaattc cgattagagt atagatatca
     5341 gtattaaaca taatacctat ataatccacc agccaatttc ctaatggtat acctgcaatt
     5401 atgcctataa ctgacattac catgttttcc ttaacaagca tcctgaatat ttcaattttt
     5461 gtaaagccca ttactctcaa ggatgaaaat tccaaggttc tttcgtttat actcatgaga
     5521 gtcatgctat atactattac aaagcccagc atacctgaaa aaataaccat aaaccccata
     5581 aacaccatta tcagtcctgt gaattcctca aagaccccct gcatttcctc ttgtgattga
     5641 attgacataa tattaattat gtcatccaat tta
//
LOCUS       DAOPUT010000089         5668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_14210_length_5668_cov_19.166756, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000089 DAOPUT010000000
VERSION     DAOPUT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14210_length_5668_cov_19.166756"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="PKK75_07065"
     CDS             complement(<1..1338)
                     /locus_tag="PKK75_07065"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNZ83397.1"
                     /translation="MKKTLLLILVMVILLNVYAYGSTGYTNISNIALYNNSSLDSGIK
                     TTLNISSRVEVLEEKDQWYRVKTLEGISGWIEKFFITLPSERYIVNNTVYNINIRTSP
                     STGSKQIGQLKPGEKAEYIDTYHSWHIIKYNGSEYYIASWLTDIEYKKSEKIYFLFDS
                     INIRSKASLSSKVISQGNSGDSFEVLGEENGWYKIALDDKSVGYVAAWLTTHDINSPS
                     SDNISYKKTTSDLNLRTGPSAAHNRILTIKKGETVKTVAGEKGWDRVITQSGNTGWCN
                     SYYLKETLPLAGKVILLDPGHGGYDPGATSYSGKFEKHLNLDVAWNLKSILNKAGAAV
                     YMTRTGDTYISNSKRGRSADKLSADILLSIHHNSLNNSDYYGMSTYYNTINYTDPSYG
                     YNLAEAIYLNAITINGVYRDGILDRNFEVLRETNTPAALIEIGFMSNPTEEMNI"
     gene            1566..2981
                     /gene="cysS"
                     /locus_tag="PKK75_07070"
     CDS             1566..2981
                     /gene="cysS"
                     /locus_tag="PKK75_07070"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNZ83398.1"
                     /translation="MKIYNTLSRTIEDFKPIDESLVKMYTCGPTVYNFAHLGNLRMYV
                     HEDVFEKTLRYLGYNVKRVMNITDVGHLESDADEGEDKMLKGAKRENKTVWEIAQYYT
                     DAFFEDINKLNIKMPDVVAKATDYIDEYIEFIKVLEEKGYTYAANGNVYFDITKVKNY
                     TKLSGMDLDSLRIATREGVELDVNKKNPQDFVLWFTKSKFENQAMKWDSPWGVGYPGW
                     HIECSVISLTNLGEEMDIHCGGVDHIPVHHTNEIAQTESYTGKDWVKYWWHGEFLIDN
                     EGKMSKSSGEFLTLSLLIKKGFNPLTYRYFVLNSHYRKQLAFSFDSIATAETAYNKLK
                     SRVKFIKDNQEESSGGNAEAFNKYKDDFENCLRDDLNTANAITVLYETLKDEELNNRE
                     KIRLVEDFEKVLSLDLLKDEEKEEVHDELASYIEEMIQKRQEAKKNKDYDLADKIRAE
                     LLEKGIMLEDTRQGVNWKKIN"
     gene            2963..3490
                     /locus_tag="PKK75_07075"
     CDS             2963..3490
                     /locus_tag="PKK75_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HNZ83399.1"
                     /translation="MEENKLNKENKSTEENKGKENNSTKENGITKENELFDKISERIN
                     STYMYSPAQLAYAGDAVYELLVRSYVLKTHDISVNKMHRLTVRFVKAKAQAYILSKLN
                     DELTDEEKKLVKRGRNAKVTSSPKNVEFMDYRYATGFETLLGYLYLNKDIDRLLYIFD
                     RIIKIILEKDDDNCE"
     gene            3481..4212
                     /gene="rlmB"
                     /locus_tag="PKK75_07080"
     CDS             3481..4212
                     /gene="rlmB"
                     /locus_tag="PKK75_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNZ83400.1"
                     /translation="MRIIEGRNPVIEALRSDAQIDTILINKEAAKGSLNKIFELAKAK
                     NILIKNVDKAVLDRLSESKNHQGVIAEAMEYEYKDIEDILAYAGEKGEMPLIVILDEI
                     TDVHNLGSIIRSAECLGAHGVIIPKRRAAQINAVAAKSSAGAVEYLPVCRVTNIADTL
                     DTLKEKGFWIYGADMEGGKYIYEEKFDVPVGLVIGSEGSGIGRLVKEKCDILVKIPMK
                     GNVSSLNASNAASIIIYEIMKQRSM"
     gene            4212..4730
                     /locus_tag="PKK75_07085"
     CDS             4212..4730
                     /locus_tag="PKK75_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNZ83401.1"
                     /translation="MARTKKEYLFIDGYNVINQWQYYRDVSGNIENSRNKLIELLVEY
                     QAYKGLKVVLVFDAHLVKGSLEKKEFISGVEVVYTKENETADSYIEKQLDRIGRYERV
                     RVATSDGAIQQIVLSRGGTRLTARELILELEGTKNDIRTKTEKPRQRGSNINQILDTE
                     TLNKLDKFRKGM"
     gene            4881..4956
                     /locus_tag="PKK75_07090"
     tRNA            4881..4956
                     /locus_tag="PKK75_07090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4914..4916,aa:Thr,seq:ggt)
     gene            4991..>5668
                     /locus_tag="PKK75_07095"
     CDS             4991..>5668
                     /locus_tag="PKK75_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ83402.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITLILNKKYGTGE
                     FIAFDHIDKAPEERERGITISTSHVEYETQKRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVSAADGPMPQTREHILLARQVGVPQLVVFLNKADMVDDPELIELVEMEVRELLS
                     EYEFDGDNIPIIVGSALGALEDPDSPWADKVLELMDAVDETIPTPARAIDKPFLMPVE
                     DVFSITGR"
BASE COUNT         1839 a          958 c         1269 g         1602 t
ORIGIN      
        1 aatattcatt tcttctgtgg ggtttgacat aaaccctatt tcaatcagtg ctgcaggagt
       61 atttgtttct ctcaaaactt caaaattgcg gtcgagaatt ccgtctctgt acactccgtt
      121 aatcgtaatt gcattcaagt aaatggcttc cgctaaatta tatccatagg atggatccgt
      181 ataatttatt gtgttgtaat aagttgacat accgtaataa tcactgttat tcaatgaatt
      241 gtgatgtatt gaaagcagga tgtcggctga caacttatct gctgacctgc ctcttttgct
      301 gtttgaaata tatgtatcgc cggtccttgt catataaacg gcagcccctg ccttattgag
      361 gatgcttttc aaattccagg caacatcaag gtttaaatgc ttttcgaatt ttccggagta
      421 gctggttgct ccagggtcat atcctccgtg cccagggtca agcagtatta cttttcctgc
      481 caggggaagg gtttctttca aataatagct gttgcaccag cctgtatttc cgctttgtgt
      541 aattaccctg tcccatcctt tttcaccggc aactgttttt acggtttcac ctttttttat
      601 ggttagaatt ctattgtgtg cagccgaagg gcccgtcctt agattcaaat cgctggtggt
      661 ctttttatag cttatattgt ccgatgaagg gctgtttatg tcatgggttg taagccaggc
      721 tgccacatat ccgacgctct tgtcatcaag agctatttta taccatccgt tttcttcccc
      781 caagacttca aaggagtcac cgctgttgcc ctgactgatt actttgctgc ttaatgatgc
      841 cttgctcctt atattgatgc tgtcaaacag aaaatatatt ttttccgatt ttttgtactc
      901 gatatctgta agccagcttg ctatgtagta ttcgcttccg ttgtatttta ttatatgcca
      961 gctgtggtat gtgtctatgt attctgcctt ttctccgggc tttagctgtc ctatctgttt
     1021 tgagcccgtt gagggggatg ttcttatatt aatgttataa actgtgttgt ttacaatata
     1081 tctttctgaa ggcagggtga taaagaattt ctcaatccat ccgcttatgc cttccagggt
     1141 tttcaccctg taccactggt ccttctcttc taagacttca actctggaac ttatattcag
     1201 ggtagttttt attcccgagt caagggatga attattatat agggcaatat ttgatatatt
     1261 tgtatatccg gtggacccat aggcatatac atttagcaat atcaccataa caagtattaa
     1321 taacagagtt ttcttcatga aattctcctt tcatccggat tttaaacatc agccgttttg
     1381 actttattac ctgactttat tagcccttaa caaaattgta attcaactga gtacatatgt
     1441 attgtaccat ttttcgacaa attttccatt acagttatat tacacgggtt gccaggatgg
     1501 aaaaattaag cttgatagaa aaagaaaagt tatatataat gagtaaagtg aaaggagtgc
     1561 tatgaatgaa aatatataat acattatcaa gaacaattga agattttaag ccgatagatg
     1621 aaagtttggt aaagatgtac acttgcggac ctacggtata caactttgcc catttgggaa
     1681 acctaagaat gtacgttcat gaggatgttt ttgaaaaaac cttaaggtac ctgggctaca
     1741 atgttaaaag ggttatgaat attacggatg taggccacct tgaatctgat gccgacgagg
     1801 gcgaagacaa aatgttaaag ggagccaaaa gggaaaacaa aaccgtatgg gaaattgcac
     1861 agtattatac tgatgccttt tttgaagata taaataaact caatatcaag atgcctgatg
     1921 tggtggcaaa ggcaactgat tacatagatg aatacataga gtttattaag gttttggaag
     1981 aaaaagggta tacatatgcg gcaaacggca atgtatattt tgacattaca aaggttaaaa
     2041 actatacaaa gctttcgggc atggatttgg atagcctgcg aattgccaca agagaaggcg
     2101 ttgaactgga tgttaacaag aaaaaccccc aggattttgt tttgtggttt accaagtcca
     2161 agtttgaaaa ccaggcaatg aaatgggatt ctccgtgggg tgtgggttat ccgggatggc
     2221 atattgagtg ctctgttata agtctcacta atttgggaga agaaatggat atacactgcg
     2281 gaggagtaga ccatattccg gtccaccata caaatgaaat agcccagaca gagagctaca
     2341 caggcaagga ttgggttaaa tactggtggc acggtgaatt cttaattgac aatgaaggta
     2401 agatgagcaa gtcaagcgga gagtttctca cgctgtcgct gctaattaaa aaaggcttca
     2461 atcccttgac ctacaggtat tttgttttaa attcacatta cagaaaacaa cttgcttttt
     2521 cctttgattc aattgccact gctgaaaccg catataacaa gctaaaaagc agggtcaagt
     2581 ttattaaaga taaccaggag gaaagttccg gcggaaatgc tgaagctttc aacaaataca
     2641 aagatgactt tgaaaattgc ctaagagatg acttgaatac tgcaaatgca ataaccgtac
     2701 tgtatgaaac gctcaaggat gaagagttga acaacaggga aaaaataagg cttgtggaag
     2761 attttgaaaa ggtcctttct cttgatttat tgaaggatga agaaaaggaa gaagttcatg
     2821 atgagctggc ttcatatatt gaggaaatga ttcaaaaaag gcaggaagca aagaaaaaca
     2881 aagattacga cttggctgac aaaataaggg ctgagcttct ggaaaaggga attatgctgg
     2941 aagatacaag acagggtgta aattggaaga aaataaatta aacaaagaaa ataaatcaac
     3001 tgaagaaaac aaaggaaaag agaataattc aaccaaagaa aatggaataa caaaagaaaa
     3061 tgaattattt gataaaatca gtgaaagaat aaacagcaca tacatgtatt cacctgcaca
     3121 gctggcttat gcaggtgatg ctgtatatga gcttctggta agaagctatg tcttaaaaac
     3181 tcacgatata agcgtgaaca aaatgcacag gctgactgtc aggtttgtaa aggcaaaggc
     3241 tcaggcttat attttgagca aattaaacga tgagcttacc gacgaagaga agaagcttgt
     3301 gaagaggggg agaaatgcca aggttacttc ttcacccaaa aatgtggagt ttatggatta
     3361 cagatatgca acgggatttg aaaccctgct gggatatttg tatttaaaca aggatattga
     3421 caggctcttg tatatttttg atagaatcat aaagataata ctggaaaagg atgatgacaa
     3481 ttgcgaataa tcgaaggaag aaatccggtg attgaagcat tgagaagcga tgctcaaatt
     3541 gacaccatat tgattaacaa ggaagcggca aaggggtctt taaataaaat attcgagctt
     3601 gccaaggcaa agaatattct gattaaaaat gtggataagg ctgttttgga caggttgagc
     3661 gaaagcaaaa atcaccaggg agttattgcg gaagctatgg aatatgaata taaggatatt
     3721 gaggacatat tggcttatgc aggggaaaag ggcgaaatgc ctttaattgt aattctcgat
     3781 gaaataaccg atgtccacaa tttggggtca attatccgtt ccgcagaatg ccttggtgca
     3841 cacggtgtaa taattccaaa gagaagagct gctcagataa atgctgttgc tgcaaaatct
     3901 tccgccggtg cagttgagta tcttcctgta tgcagagtta ctaatatagc agacacactt
     3961 gatacattaa aggaaaaagg attttggata tacggagctg acatggaagg cggcaaatat
     4021 atatatgaag agaaatttga tgttcctgtg gggcttgtta ttggaagtga aggctctggc
     4081 atagggaggc tggttaagga aaagtgcgat attctggtta aaatacccat gaagggaaat
     4141 gttagttctt taaatgcttc taatgcggct tcaattatta tatatgaaat aatgaaacaa
     4201 aggtcaatgt gatggcaaga acaaagaagg agtatctttt tatagacgga tacaatgtaa
     4261 taaatcaatg gcagtattac agggatgttt cgggtaatat cgaaaactca aggaacaagc
     4321 ttattgagct gctggttgaa taccaggcat acaaggggct taaggttgtt cttgtgtttg
     4381 atgcccacct ggtaaaagga agcctggaaa agaaggagtt catttccggt gtagaggttg
     4441 tgtacacaaa ggagaatgaa acggcagaca gctatattga aaagcagttg gatagaatag
     4501 gaaggtatga aagggtccgg gttgccacat ctgacggtgc gattcagcag attgtcctgt
     4561 caaggggagg gacaaggctt actgcccggg aactcatatt ggaactggaa gggaccaaaa
     4621 atgatattcg tacgaaaacc gaaaagcccc gtcaaagagg aagcaacatc aaccagatac
     4681 tggatacgga aacattgaac aagcttgata aattcagaaa agggatgtaa gcttttaaaa
     4741 agcattttaa ctttgttata ctgagtgaaa ggcagtatct cgtccggaaa ttcattgcac
     4801 tgacatagaa attttactat tgatttattt tctcattatt gatataataa agcttgttaa
     4861 caacaaggat tagacagcat gcttatgtag ctcaggcggc agagcacttc cttggtaagg
     4921 aagaggtcag cagttcgaat ctgctcataa gctccaaaat tataaaattt aaatccggga
     4981 gggattgaaa atggcaaaag caaaatttga aagaacaaaa ccgcatgtta atatcggtac
     5041 aatcggccac gttgaccacg gcaaaacaac gttgacagcg gcaatcacgc tgatattgaa
     5101 caaaaaatac ggaaccggag aattcatagc atttgatcat atagacaaag ctccggaaga
     5161 aagagaaaga ggaataacta tttctacttc tcacgttgaa tatgaaactc aaaaacgaca
     5221 ctatgcacac gttgactgcc cgggacacgc cgactacgtt aagaacatga ttacgggagc
     5281 cgcccagatg gacggagcaa tcctggttgt atcagctgct gacggcccta tgccccagac
     5341 aagagagcat attctgcttg caagacaggt tggagttcct cagctggttg tatttttaaa
     5401 caaagctgat atggttgatg accctgaatt gattgaattg gttgagatgg aagttagaga
     5461 gcttttatct gaatatgaat ttgacggcga caatattcca ataattgtag gctcagcatt
     5521 aggagcattg gaagatccgg acagcccatg ggctgacaag gttcttgaac tgatggatgc
     5581 tgttgatgag acaataccca cacctgcaag agctattgac aaacctttcc tgatgcctgt
     5641 agaagacgtg ttctcaatca caggaaga
//
LOCUS       DAOPUT010000090         5477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_14881_length_5477_cov_19.204906, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000090 DAOPUT010000000
VERSION     DAOPUT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14881_length_5477_cov_19.204906"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1139
                     /locus_tag="PKK75_07100"
     CDS             84..1139
                     /locus_tag="PKK75_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ83403.1"
                     /translation="MEKPANKKLSSSELSFFCMQIAMVLKSGMLIKDGIEWMHDDIEE
                     GTIKNALYSIKNRMSEKVPLHDAMKDSGYFPSYIVNMSQIGSVTGKLENVMESLSLYY
                     DRENFLKGKIKNSIFYPAILFVMMSFVIILLVTKIFPVFEGMLEELGASPDDTSFLVS
                     FSKGITAGRIAMVLVIVIMAVVALIFILNKTENGKKTLNNFLNNFSLTKNIMKKITAY
                     RFSSAMSLLLNSGMNVDSSIDMLLDIVEEPVLKEKIQSCRDSMKSGDGFLESVSKLSL
                     FSGMHMQMLNMGQKIGEMDLVMNKLTDIYENEAEQAINNTVSLIEPVLVGILSVLIGV
                     ILISVMLPLMNIMSSIG"
     gene            1242..1544
                     /locus_tag="PKK75_07105"
     CDS             1242..1544
                     /locus_tag="PKK75_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83404.1"
                     /translation="MKNYFKFFSVIIIIMLVAFMYTAVDNAQRKTGEESHKILSDAII
                     RSAVQCYAIEGFYPPDIKYLEDNYGLLVDHDRYFVSYSVFASNIIPQVQVFLKYAQ"
     gene            1561..2049
                     /locus_tag="PKK75_07110"
     CDS             1561..2049
                     /locus_tag="PKK75_07110"
                     /inference="COORDINATES: protein motif:HMM:NF027478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HNZ83405.1"
                     /translation="MDTKNQFSFQFIFVMLLYLIIVILSVMIISLGKNIYDRINKDRN
                     TNYELRVSLSYIANKIRQSDIDKAVEITKLNGVDAVLINELFDDEKYQTWIYFYDGAI
                     YEMFTDADTAFELSDGMKVVDADDFNIEKVRDDMYKFTAVNNEESSDLYLSLYSKAAG
                     HE"
     gene            2042..2515
                     /locus_tag="PKK75_07115"
     CDS             2042..2515
                     /locus_tag="PKK75_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83406.1"
                     /translation="MNKVALNSKVSLVEIILSILIFAVAGIIMLSCFGIARFTQIRAN
                     DKTEAGMIIQSDFEVIKSLKSTVEMHEFLDESYEEKEPGNIYTKYYDENWKQGSDRDY
                     SVTIVIDQENTRSGDLVSINISAEREKPYPFINKGIREIYSIESRKFFPYGGSHE"
     gene            2508..2975
                     /locus_tag="PKK75_07120"
     CDS             2508..2975
                     /locus_tag="PKK75_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83407.1"
                     /translation="MNRRTNVSVGMGGTLIITVFVVLCLMIFAVLSFTTAYSDLKLSN
                     KAQEITADYYSIHGRAEEKLAEISDVLYDTDVNESKESILRKLSDIDGIDIDEENLNI
                     QYEVSGEKNQKITVALNLFFTDRANYEIRAWNLSNIELPVYEDEIIDIWEGID"
     gene            2977..4035
                     /locus_tag="PKK75_07125"
     CDS             2977..4035
                     /locus_tag="PKK75_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNZ83408.1"
                     /translation="MDIMNMFTTAIEKKASDIFIIAGRPLSYKILGDIVTFNDEPLSS
                     DDTYGIISDIFSIANEKDLCFLEDEGDVDFSFSLPKLGRFRVSAYKQRNSYAAVIRVV
                     FFELPDPKKLGIPDVVMNLYKKTKGLVLITGPAGSGKSTTLACLIDKINKERNCHIMT
                     FEDPIEFIHKHKKSIVSQREIYTDTKSYISSLRSALRQAPDVLLIGEMRDLETIEIAL
                     TAAETGHLVLSTLHTTGAANTIDRIIDVFPSNQQQQVRVQLSMQLQAVISQHLIPSAV
                     AGRAAAFEVMLTNNAISNLIRDSKVHQLNSIIYSSEYMGMKAMDVSILELYQKGLITK
                     ENALVYATEPDSLIKHLK"
     gene            complement(4164..5033)
                     /locus_tag="PKK75_07130"
     CDS             complement(4164..5033)
                     /locus_tag="PKK75_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNZ83409.1"
                     /translation="MKRITAIIMVLLFIASFTLCYGADDIYKGTKSEIDASKTEDGYV
                     KIKYLNETTKKLKVIIEKGTGKYTYDLNNKGEYDIYPLQMGDGKYKIRVFENLSGIKY
                     ATKQTVTIDVKLKNENAPFLAPNQLVNFEETCEAIKKAEELTEGLTEDFEKIDVIYDY
                     IISNIKYDTEKARTVKSGYLPSVDDILKSSKGICFDYASLMAAMLRSRDIPAKLVTGY
                     SSNLNVFHAWNEVYTDETGWIKLNEMYFDGYQWKLMDSTIASTGKQSNSPKVMEYTNK
                     LIDSKFYTKQFEY"
     regulatory      complement(5105..5191)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1742 a          923 c         1074 g         1738 t
ORIGIN      
        1 aacaagaatt cgtgtagaat tttgttgaat tttgaataca cctataatat aattattaaa
       61 aagccaacaa ttgtgaggtt gttatggaaa aaccagcaaa taagaaattg tcttcatctg
      121 aattgtcatt tttctgcatg cagattgcta tggtattgaa atcgggaatg cttattaaag
      181 acggcattga atggatgcat gatgatatag aggaaggaac cataaaaaat gctctttatt
      241 ctataaaaaa ccggatgtct gaaaaggtcc ctctccacga tgcaatgaaa gactccggat
      301 atttcccttc atatatagta aatatgagtc agataggaag tgtaaccggc aagcttgaaa
      361 atgtaatgga gtctttatct ttgtattacg acagagaaaa tttcctgaaa ggaaaaatta
      421 aaaattccat tttttatccg gcgatacttt ttgtgatgat gtcttttgta attatattat
      481 tggttactaa aattttccca gtatttgaag gaatgcttga agagcttggt gcttccccgg
      541 atgatacgtc atttttggtg tccttctcaa agggcataac agccggaaga attgccatgg
      601 tgctggtaat cgtaataatg gcggttgttg ctttaatttt tattcttaat aaaactgaaa
      661 acggcaaaaa aaccctcaat aattttttaa acaatttttc actgaccaaa aacattatga
      721 aaaaaattac cgcataccgt ttctcatcag cgatgtccct tttgttaaac agcggcatga
      781 atgtggattc ttcgatagat atgcttttgg atattgttga agagccggtt cttaaggaaa
      841 aaattcaatc ctgcagggat tcaatgaaat caggagacgg tttccttgaa tcggtaagta
      901 agttatccct gttttcaggc atgcatatgc agatgctgaa catggggcag aaaatcggtg
      961 aaatggacct tgtaatgaat aagcttaccg atatatatga aaacgaagca gagcaggcaa
     1021 tcaacaatac tgtttcattg attgaaccgg tgctggtagg tatcttgtct gtattaatag
     1081 gagtgatact gatttccgtg atgcttcctt taatgaacat aatgtcgtca atcgggtaat
     1141 tttaagaatc acctgatggg aaaaaatccg ctgatgaaca taatgtcgtc tatggggtag
     1201 attaagcagt catctatcag ataattggaa ggaatgattt tgtgaaaaat tattttaaat
     1261 ttttttctgt tattataata ataatgctgg ttgcttttat gtacacggcg gttgataatg
     1321 cccagagaaa aaccggcgaa gaaagccata aaattttatc tgatgcaata ataaggagtg
     1381 ctgttcagtg ctatgccatt gaagggttct accctcccga catcaaatat cttgaggaca
     1441 actacggttt gctggtagac catgacaggt attttgtaag ctacagtgtt tttgcctcca
     1501 atataatacc ccaggtgcag gtatttctga aatatgcaca gtaaagagga aggaagaaat
     1561 atggatacaa aaaaccagtt ttcatttcaa tttatatttg taatgcttct ctaccttatt
     1621 attgtaatat tgtcagtaat gattatttcc ctggggaaaa acatatacga caggataaat
     1681 aaagatagaa acacaaatta tgaactcagg gtttctcttt cttacattgc caataagata
     1741 aggcagtctg acattgataa agctgttgaa atcaccaagc tgaacggcgt agatgcagta
     1801 ttgataaatg aattattcga tgatgaaaaa tatcaaacat ggatatattt ttatgatgga
     1861 gcaatttatg aaatgtttac agatgcggat accgcctttg aattgtcgga cggtatgaag
     1921 gtagttgatg cagatgattt taatattgaa aaggtcaggg atgatatgta caagtttact
     1981 gcagtgaaca atgaagaaag ttcagatctg tatttatccc tgtattcaaa ggcggcgggc
     2041 catgaataaa gtagcactta attcaaaggt ttcactggtt gaaataattt tatccattct
     2101 gatatttgcc gtggcaggaa taataatgct aagttgcttc ggtattgcaa ggtttaccca
     2161 aataagggca aacgacaaga cagaagcagg catgatcatt cagtcggatt ttgaagttat
     2221 caagtcatta aaaagcactg ttgaaatgca tgaattcctg gatgaatctt atgaagaaaa
     2281 agagccaggt aacatataca ccaagtatta cgatgagaac tggaaacagg gaagcgacag
     2341 ggattattcc gtaacaatag taatagacca agaaaataca aggtccggag acctggtaag
     2401 tataaatatt tcagctgaaa gggaaaaacc ctatcctttt ataaataagg gtatccggga
     2461 aatctattct attgaaagca ggaagttttt cccttatgga ggcagccatg aatagaagga
     2521 ccaatgtttc ggtgggcatg ggaggaaccc ttataatcac tgtgtttgta gttttgtgcc
     2581 tgatgatttt tgccgtattg tcatttacaa ctgcctattc ggatttgaag ctgtctaaca
     2641 aagctcagga aatcacagcg gattactata gtatacacgg cagggctgaa gaaaagctgg
     2701 cagaaatcag cgatgtgctg tatgatactg atgtaaatga aagcaaggaa agtattctca
     2761 ggaagctgtc agatattgac ggaattgaca ttgatgaaga aaatttaaat attcaatatg
     2821 aagtatcggg agagaaaaat caaaaaatca ccgttgcttt aaacttattt tttacagaca
     2881 gagctaatta tgaaattagg gcgtggaatt tgtcaaatat cgagctgcct gtttatgaag
     2941 acgaaataat tgatatatgg gaagggattg attagtatgg atataatgaa tatgtttaca
     3001 actgccatag agaaaaaagc ttcggatatt tttataatcg ctggcagacc cttaagctat
     3061 aaaatcttgg gagatattgt gacctttaat gacgaaccgc tttcttccga tgatacatac
     3121 gggataataa gtgatatttt cagcattgcc aatgaaaaag acctttgttt tttagaagat
     3181 gaaggagatg tggatttttc attttcactg cctaagcttg gaaggttcag ggtaagcgcc
     3241 tacaagcagc ggaattccta cgctgcggta atcagggttg tgttttttga actgcctgac
     3301 cctaagaagt taggcatacc tgatgttgta atgaatctat acaaaaaaac caaggggctg
     3361 gttcttataa caggtccggc aggcagcgga aagtcaacaa ccctggcatg tttgatcgac
     3421 aaaatcaaca aggagagaaa ctgccacata atgacctttg aagaccccat agaatttatt
     3481 cacaaacaca aaaagtcgat tgtaagccaa agagaaattt acacagatac caaaagctat
     3541 atttcttccc tccggtctgc attgagacag gctcctgatg tattgctcat aggtgaaatg
     3601 agggatttgg aaactattga aatcgctctt actgctgcag aaacagggca tttggtttta
     3661 tccacactgc ataccaccgg tgccgctaat accattgaca gaattattga tgttttcccg
     3721 tccaatcagc agcagcaggt gagggttcag ctttcaatgc agctgcaggc ggtgatttca
     3781 cagcacctca ttccttcagc tgttgcagga agggctgcag cctttgaggt tatgctcaca
     3841 aacaacgcca tatccaattt aataagagat tccaaggtcc accagctaaa cagcataatt
     3901 tattcttcgg aatatatggg tatgaaggcc atggatgtaa gtattttgga attatatcaa
     3961 aagggtttga taacaaagga aaatgcattg gtatacgcca ctgaaccaga ttctctcata
     4021 aaacatctga aatagtatat actaaaaaat gacctattgg gggaaggtca ttttttagtg
     4081 tggtgaggag actaattaat atctttttaa tattaggaag ggcatatata acccgcctac
     4141 ccccttgaaa tgcgggatta tagttaatat tcaaactgtt tcgtataaaa tttgctgtca
     4201 attaatttat ttgtatattc cataaccttg gggctgtttg actgtttgcc tgttgatgcg
     4261 atggtagagt ccataagctt ccactgatat ccgtcaaaat acatttcatt cagctttatc
     4321 cagcctgttt catcggtata cacttcattc catgcgtgaa atacatttaa atttgaagaa
     4381 tagccggtta ccagtttagc cggaatatcc cgggaccgaa gcattgcagc cattaatgaa
     4441 gcataatcaa agcaaatacc cttgctgctt ttcagtatat catctactga aggcagatat
     4501 ccgcttttca ctgttcttgc tttttcggtg tcatatttaa tattgctgat tatataatca
     4561 taaataacat ctattttttc gaagtcctct gtcaagcctt ctgttaattc ttcagctttt
     4621 ttaattgctt cacatgtctc ttcaaaattc accagttgat tcggcgctaa aaatggcgca
     4681 ttttcattct tcagcttaac gtctattgta actgtctgct ttgttgcgta cttaattccg
     4741 cttaaatttt cgaatactct tattttgtac tttccgtccc ccatttgcag aggatatatg
     4801 tcatattcgc ctttgttgtt taaatcgtat gtatatttac cggtgccctt ttcaattata
     4861 accttcagct tttttgttgt ttcatttaaa tattttattt ttacgtatcc gtcttccgtt
     4921 tttgaagcat caatttccga ttttgtaccc ttgtatatgt catctgcccc atagcaaagt
     4981 gtaaatgaag ctataaataa caataccatt attattgctg taattctttt cataattttc
     5041 ctccccaata agaaaagctt tgccggtcag caaagctata tatcaattta atgtacccaa
     5101 taagtacagc aactggctgt cataccattt tcatggctta gaccctatag ctttgcgcca
     5161 ttttctttag aaaattttgc cctttattta attatggtaa tcctaacaca attcttttga
     5221 attgtcaaca tattttgaca atgttttccg aatattttcc aaaaactttt ttcagattcc
     5281 tctgaaatat ttgcaaaaat ccggagtaaa tgttactatt cacacccaat ggcttgtcat
     5341 tatagatggg actttctgaa aagaaagaaa tatagtagtt atgttcactg aaacttgtct
     5401 ttgctgtgat aataatcacg ggaggagttt tgaaatttaa aaaaaacacg gaaacttaat
     5461 tccgtgtttt ttatgtg
//
LOCUS       DAOPUT010000091         5352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_15359_length_5352_cov_18.513687, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000091 DAOPUT010000000
VERSION     DAOPUT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15359_length_5352_cov_18.513687"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..684
                     /gene="rpsJ"
                     /locus_tag="PKK75_07135"
     CDS             373..684
                     /gene="rpsJ"
                     /locus_tag="PKK75_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNZ83410.1"
                     /translation="MANTQKIRIRLKAYDHQLIDQSAQKIVETAKSTGATVAGPIPLP
                     TEKQIITILRAVHKYKDSREQFEQRTHKRLIDITNPTPKTVDSLMKLNLPAGVDIEIK
                     L"
     gene            768..1400
                     /gene="rplC"
                     /locus_tag="PKK75_07140"
     CDS             768..1400
                     /gene="rplC"
                     /locus_tag="PKK75_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNZ83411.1"
                     /translation="MKAILGKKIGMTQVFTEQGDVVPVTVLESGNMVVVQKKTEEKDG
                     YNAIQVGFGDTKIKNVIKPKKGHFDRSKVEPKRFLREIKVDNIDDYEVGQKIGVDVFK
                     AGEKVDVSGISKGKGFQGPVKRHGHARGGMSHGSKFHRLRGSLGASAGMGKVVKGMKA
                     HGHMGNEKVTVLNLEVVRVDADKNVILLKGAVPGPKKGLVKIREAVRKNK"
     gene            1427..2050
                     /gene="rplD"
                     /locus_tag="PKK75_07145"
     CDS             1427..2050
                     /gene="rplD"
                     /locus_tag="PKK75_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNZ83412.1"
                     /translation="MPKVALYDITGSQIGDVELSDDIFGVEVNTHVMYEAVKNYLANQ
                     RQGTQSAKTRGEVRGGGRKPWRQKGTGRARQGSTRSPQWVGGGVVFAPKPRDYSYKLP
                     KRIKRLALKSALSSKVQDNEIIVVDSLVLDKPKTKDMIKVLLNLKAGKKTLVVIPERD
                     ENVILASRNIPGVKTAYVNTINVYDILNCDSFLITRDAVNKVEEVYA"
     gene            2050..2340
                     /gene="rplW"
                     /locus_tag="PKK75_07150"
     CDS             2050..2340
                     /gene="rplW"
                     /locus_tag="PKK75_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNZ83413.1"
                     /translation="MRDPHDIILKPIVTEASMNAMADKKYTFVVDKKSNKTEIKNAVE
                     SIFGVTVASVNTMNMLGKNKRMGIHVGKKADWKKAIVTLTEDSKTIEFFEGI"
     gene            2365..3198
                     /gene="rplB"
                     /locus_tag="PKK75_07155"
     CDS             2365..3198
                     /gene="rplB"
                     /locus_tag="PKK75_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNZ83414.1"
                     /translation="MGIRKYRPTSPALRQMTVSTFEEITTVEPEKSLLAPLKSNAGRN
                     AHGRITVRHKGGGEKRKYRIIDFKRNKDGVPGIVATIEYDPNRSSYIALINYVDGEKR
                     YILAPNKLQVGDTIESGPEADIKIGNTLPVGSIPVGTMIHNIELKPGKGGQMVRSAGN
                     AAQLMAKEGKYAQVRLPSGEVRMILMDCRATIGQVGNIDHENVSIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPVTPWGKPTIGYKTRKKNKKSDSMIVSRRNK
                     K"
     gene            3225..3506
                     /gene="rpsS"
                     /locus_tag="PKK75_07160"
     CDS             3225..3506
                     /gene="rpsS"
                     /locus_tag="PKK75_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNZ83415.1"
                     /translation="MGRSLKKGPYCEPSLMKKIVEMNDANNKKVLKTWSRRSTIFPEM
                     IGHTLAIHDGRKHVPVYITEDMVGHKLGEFAPTRTYRGHDKSDKTSKVK"
     gene            3523..3858
                     /gene="rplV"
                     /locus_tag="PKK75_07165"
     CDS             3523..3858
                     /gene="rplV"
                     /locus_tag="PKK75_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNZ83416.1"
                     /translation="MEAKAVAKYMRISPRKMKFVCDMVRGKQVDEALAILKFHPGKGA
                     KILEKVVKSAAANAENNFDMDRDKLYVSNAYANQGPTLKRWRPRAKGSAYKILKRSSH
                     VGAVLKERE"
     gene            3879..4631
                     /gene="rpsC"
                     /locus_tag="PKK75_07170"
     CDS             3879..4631
                     /gene="rpsC"
                     /locus_tag="PKK75_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNZ83417.1"
                     /translation="MGQKVNPHGLRVGIINDWDSKWYADKKNFGDNLTEDYKIREYIK
                     KENFQSGIAKIEIERFASRVKVSVFTAKPGMIIGKGGTGVDSLKSSIERITGKTVIIN
                     VEEIKVPELNGQLVAENIASQLEKRISFRRAMKQAMQRSMKVGAKGIKTSVSGRLNGA
                     DMARTEGYSEGKIPLQTLRSDISYGFAEADTTFGKVGVKVWICRGEILRKPGSLLSDY
                     QEPARALNDNRDAKKKRRPGGKPGNFKRDKKQ"
     gene            4657..5100
                     /gene="rplP"
                     /locus_tag="PKK75_07175"
     CDS             4657..5100
                     /gene="rplP"
                     /locus_tag="PKK75_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNZ83418.1"
                     /translation="MLMPKRVKRRKQQRGRMTGIATRGNTITYGEYGLQAIEPAWITS
                     NQIEAARRAMSRAVKRGGQIWIKIFPDKPVTKKPAETRMGKGKGSPEYWVAVVKPGRV
                     LFEMSGVTEDVAREAMRLAMHKLPIKCKFVAKDQAAEKDGEANES"
     gene            5090..5293
                     /gene="rpmC"
                     /locus_tag="PKK75_07180"
     CDS             5090..5293
                     /gene="rpmC"
                     /locus_tag="PKK75_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNZ83419.1"
                     /translation="MKAKELRNKSVDELNKSVVDLKTELFNLRFQLATGELDNPMRIN
                     KVKKDIARVKTVIRERELNINME"
BASE COUNT         2068 a          804 c         1280 g         1200 t
ORIGIN      
        1 ttatttttat tatttttaca atagaatggc aagggtacaa gggtgacatc atgttttgga
       61 acggaaagaa tcgtccccaa aattgatatt tgtgaaggaa atttaataaa tattaagtta
      121 tagaaataaa ttaaaaaaac cagaaaaaat gcttgattta tagtatttga tagtatatta
      181 ttaaaaagct tgccacatgc gatgacgtaa aaggttgctg aaattttaac gtttcaggga
      241 atttttacag agtatgtcct gctaaaagca gggcgaccgg taccgctttt ataaaaaaaa
      301 atatggaaca cccggatggg tttatcgagc ggttccgagt tgtatggaaa catacgaaaa
      361 ggaggtattt aaatggcaaa cacacagaaa ataagaatca ggttaaaggc ttatgatcat
      421 caattaattg atcaatctgc gcaaaaaatc gtggaaacgg ctaaaagtac gggtgcaacc
      481 gttgcaggtc caatcccgct gcctacagaa aaacaaataa ttactatact gagggcagtt
      541 cacaagtaca aggattccag agagcagttt gaacaaagaa cacataaaag attgattgac
      601 ataacaaatc ctactccaaa aactgtggat tcgcttatga agctgaatct gccggcaggg
      661 gtagacattg aaatcaagtt ataaaaacaa gtagcaagta gagaaataac acactctgct
      721 tagaatgatt gcaaagcaat ccgctgtaag aaaccaggag gtgtagaatg aaagcaattc
      781 taggaaaaaa gattggaatg actcaagtgt tcactgaaca aggagatgtt gttcctgtaa
      841 ctgttttgga atccggcaac atggttgttg ttcagaagaa aacagaagaa aaggacggat
      901 acaatgcaat acaggttgga ttcggagaca cgaaaattaa aaatgttatt aaaccaaaga
      961 aaggtcattt tgatagatca aaggttgagc ctaaaagatt tttgagagaa atcaaagtag
     1021 ataacattga cgattatgaa gttggtcaga agataggcgt tgatgttttc aaggcaggag
     1081 aaaaagttga cgtttcaggg atttcaaagg gcaagggatt tcagggaccg gtaaaaagac
     1141 acggacatgc aagaggcggc atgagccacg gttcaaaatt ccacaggctg agaggctcat
     1201 tgggtgcttc ggcaggcatg ggaaaagtcg ttaaaggaat gaaagctcac ggccacatgg
     1261 gaaatgaaaa agttactgtg ttaaaccttg aagttgtaag agtagatgca gataaaaatg
     1321 taatactttt aaaaggtgca gtaccgggac ctaaaaaagg tttggtaaaa atcagagaag
     1381 cagttagaaa aaataagtaa taattgcatg gaaaggagga ttaaagatgc caaaagtagc
     1441 tctttatgat ataacaggca gccaaatagg cgacgtggag ttaagtgatg atatattcgg
     1501 cgtagaggtt aacactcacg taatgtatga agccgtgaaa aattatttgg ctaatcaaag
     1561 acaaggaact cagtctgcca agactagggg agaagtaaga ggcggcggaa gaaaaccctg
     1621 gagacaaaaa ggaacaggcc gtgcaagaca aggaagcacg agatcgcctc aatgggtagg
     1681 cggaggagtt gtatttgctc caaaaccaag agattacagc tacaaactac caaagagaat
     1741 taaaagactg gcgctgaaat cagcattaag ctcaaaagta caggataatg aaattatagt
     1801 agttgacagc ttggttcttg ataaaccgaa aacaaaggat atgataaagg tgttattaaa
     1861 tcttaaagca ggaaagaaaa cattggtggt tattcctgaa agagatgaaa atgtaatact
     1921 agcatcaaga aatatacccg gcgttaaaac agcatatgta aatacaatta atgtatacga
     1981 tatattaaac tgcgattcat tccttataac cagggacgca gttaataaag tggaggaggt
     2041 gtacgcataa tgcgcgatcc acacgatata atcttaaaac cgatagtgac agaagcaagt
     2101 atgaatgcta tggcagataa aaaatacact tttgtcgttg ataagaagtc taacaagact
     2161 gaaatcaaaa atgcagtgga atcaattttc ggagttacag tagccagtgt gaacacaatg
     2221 aacatgctcg gcaagaataa gagaatgggt attcatgtgg gcaagaaagc cgactggaaa
     2281 aaggccattg taactttgac agaagacagt aaaacaatag aattctttga agggatttga
     2341 aattagtaga aggaggaaaa tctcatgggt atcagaaagt acagaccaac ttctccggcg
     2401 ttgagacaaa tgaccgtttc aacatttgaa gaaataacaa ctgtagaacc tgagaaatct
     2461 cttttagctc cgttaaaaag caatgctgga agaaacgcac acggaagaat tacggttcgt
     2521 cacaaaggcg gcggagaaaa gagaaaatat agaattatcg attttaaaag aaacaaagac
     2581 ggagttccgg gaatagttgc aacaatagag tatgatccaa acagaagctc atacattgca
     2641 cttataaatt atgttgacgg agaaaaaaga tatattcttg ctccaaacaa actgcaggtt
     2701 ggagatacaa ttgaatcagg tccggaggca gacattaaaa tcggaaatac acttcctgta
     2761 ggaagtatcc ccgttggtac aatgattcac aatattgaac taaaacctgg aaaaggcggg
     2821 caaatggttc gttctgcagg aaacgccgct cagcttatgg ccaaggaagg caaatatgca
     2881 caggtaagac ttccaagcgg tgaagttcga atgatattaa tggactgcag agctacgatc
     2941 ggtcaggtag gaaacataga ccacgagaat gtatccatag gcaaggcagg aagaaagaga
     3001 catatgggaa tcaggcctac ggtaagaggg tcggtaatga atcctaatga tcacccccac
     3061 ggcggcggtg aaggaagagc accaatagga agaccatcac cggttacacc ttggggaaaa
     3121 ccgacaattg gatataaaac tcgtaagaag aacaaaaaat cagatagtat gatagtaagc
     3181 agaagaaata agaaataagc aggattgaaa ggaggaaata gtgaatgggt agatcgttaa
     3241 agaaaggacc ttattgtgaa ccaagcctta tgaagaaaat tgttgaaatg aatgatgcaa
     3301 ataataagaa agtattgaag acatggtcaa gaagatcaac aattttccct gaaatgatag
     3361 gccatacatt agctatacat gatggcagaa agcacgttcc tgtatatata actgaagata
     3421 tggtcggaca taagctgggt gaatttgctc cgacaagaac atacagagga catgataagt
     3481 ccgataaaac ctcaaaagtt aaataagaag ggaggcagct aaatggaagc taaggcagtt
     3541 gctaaatata tgagaatatc accgagaaag atgaaattcg tgtgtgatat ggtaagaggc
     3601 aagcaagttg acgaagcatt ggcaatttta aaatttcatc ccggcaaggg ggcaaaaatt
     3661 ttagaaaagg tggttaagtc tgctgcggct aatgcagaaa acaactttga catggataga
     3721 gataaattat atgtttcaaa tgcatatgca aatcaagggc cgaccctcaa aagatggaga
     3781 cctagagcaa agggttcggc atataaaata ctaaaaagat caagccacgt aggcgcagtc
     3841 ttaaaagagc gagaatagac gagtaaagga ggtaaaaaat gggtcaaaag gtaaaccctc
     3901 atggactaag agtcggtata atcaatgatt gggattcaaa atggtatgct gataaaaaga
     3961 atttcggaga taatttaact gaagactata aaattcgtga atatataaag aaggaaaact
     4021 ttcaatccgg tatagcaaaa atagaaatcg aaagatttgc cagccgtgtt aaagtaagtg
     4081 tattcactgc aaaacccggc atgattatcg gaaaaggcgg tacaggagtc gacagtttaa
     4141 aatccagtat agaaagaatt acaggaaaga ctgtaataat taatgtagaa gaaataaagg
     4201 ttcctgaatt aaacggccag ctggtagcag aaaatatagc atctcagctt gaaaagcgta
     4261 tttcattcag aagagcaatg aagcaggcaa tgcaaagatc aatgaaggtg ggcgcaaagg
     4321 gaattaaaac aagtgtatcc ggaaggttaa acggagctga tatggcaaga accgaaggat
     4381 attcggaagg caaaattcct cttcaaactt taagatctga cataagctat ggttttgcgg
     4441 aagcagatac aacattcggc aaggtcggtg tcaaggtttg gatatgcaga ggtgaaattt
     4501 taaggaaacc gggttcattg ttgtctgatt accaagaacc tgcaagagca ttaaatgata
     4561 acagagatgc caagaagaaa agaagaccgg gtggcaaacc aggtaatttt aagagagata
     4621 aaaagcagta attcctgaag gaaggaggaa ataattatgt taatgcctaa aagagtaaaa
     4681 cgccgtaaac agcaaagagg cagaatgacc ggtattgcaa caagaggcaa tactatcaca
     4741 tacggcgaat atggtctgca ggcaattgag ccagcttgga taacatcaaa ccaaatcgaa
     4801 gcagccagaa gggctatgtc aagagcagta aagaggggcg gccagatttg gattaaaata
     4861 tttccggata aaccggtaac aaaaaaacca gccgaaacac gtatgggtaa aggcaagggg
     4921 tcacctgaat actgggtagc agtagtaaaa ccgggcagag tattatttga aatgtcaggg
     4981 gttactgaag atgttgcaag agaagcaatg agacttgcca tgcataaatt gcctatcaaa
     5041 tgcaagtttg ttgccaagga tcaggcagca gaaaaggatg gtgaagcaaa tgaaagctaa
     5101 agaattaaga aataaatcag ttgatgagtt gaacaagtct gtagtggatt taaagacaga
     5161 gctgttcaat ctgagatttc agcttgcaac cggggagctt gataacccta tgagaataaa
     5221 taaagtcaag aaagatattg ctcgggttaa aacagtgatt cgcgaacgcg agttaaacat
     5281 taacatggag taaaagaaag gaggatttac tttggaaaga ggcaacagaa aagtaagaat
     5341 cggtaaagta gt
//
LOCUS       DAOPUT010000092         5268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_15685_length_5268_cov_20.252446, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000092 DAOPUT010000000
VERSION     DAOPUT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15685_length_5268_cov_20.252446"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKK75_07185"
     CDS             <1..186
                     /locus_tag="PKK75_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SanA protein"
                     /protein_id="HNZ83420.1"
                     /translation="GVDAYGVSADPRLYAGQKFREAREIAARAKDFVKCIFKPPAAIL
                     GETIPVSGDGNVTNDKY"
     gene            292..1281
                     /locus_tag="PKK75_07190"
     CDS             292..1281
                     /locus_tag="PKK75_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNZ83421.1"
                     /translation="MYHARFKGRHYEAGLKWGKLLLKNGIKLDFSPTFELTEERFRFA
                     EKCLDEYKRYFPEILDEIRGIADGNEVPENVLQTILFSMYCFELDNRCTVFAISTEDE
                     IIFGRNSDFLKSLEKLYMNCIYRLTGSNSFNANTTAYVQMEDGINEHGLATGLTFVYP
                     RIRKPGLNAGMLVRYLLEKCSTTKESVEELHRLPIASAQTITLADKKGDIAVVECNPE
                     NIVVIEPEPGEQFVATANNFYSVQMRGWRNGDIDDWRSDERYQTAFSALKNNKGNYSV
                     DLAKSILSGKYGFMCQYDRKGIADTVWSVVYDIKHDRIWRVEGNPSRKKFREI"
     gene            1389..2510
                     /locus_tag="PKK75_07195"
     CDS             1389..2510
                     /locus_tag="PKK75_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ83422.1"
                     /translation="MKEQLLSIRKINKYFGRSHILKDVTLEIKDGEFITLLGPSGCGK
                     TTILRCIAGLESIDSGDILLYGESIKDLAPNKRNVNTVFQNYALFPHLNVYDNIAYGP
                     RIKKSIGEGEIKKSVAEMLELVQMQGYEKRMTHQLSGGQRQRIAIARALINKPDILLL
                     DEPLGALDLKLRKYMQTELAHLQKQAETTFIYVTHDQEEALNMSDRIVVINEGVVQQV
                     GTPSDIYNNSKNLFVAGFIGDRNIKEVKVLQKKGDFCSVKLGNSVIYIKPDRSLVDHD
                     KCSEMEAAVLAVHMDKMKISYEEVPNSLYGQITFVHYAGSQIKTEVDVDGELLTVIEY
                     QNDNDNYSIGDKVYVSWEETGVLLLPKEDTTGRREDEKD"
     gene            2497..3345
                     /locus_tag="PKK75_07200"
     CDS             2497..3345
                     /locus_tag="PKK75_07200"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83423.1"
                     /translation="MKRIKQNLPALPILGYLLLLVGIPLLLVVILSFLSRDSLGNIIF
                     EFTPDNYRKMFDPVYLRVFLNSFSLALFTGLITLLVGYPVAYFTAGLEQKRRSFVILL
                     IMLPFWISSLLRTYGWTILLGKEGLINNVLMFFNIIDQPLNMMYKFGTVLTGTAYMLV
                     PFMIISIYNSVDKLDRSLLEASYDLGGGKWKTFFNITLPLTTPGIARGFTLVFIPALG
                     LYYISDLLGGGNTMFLGNLINNMATRGRNRPLASAFAVGMILLVLLVLFIYGRVTKSG
                     KESLYQ"
     gene            3342..4145
                     /locus_tag="PKK75_07205"
     CDS             3342..4145
                     /locus_tag="PKK75_07205"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83424.1"
                     /translation="MNIKQKKFRTFYLSLFFIILYVPVLSVVLYSFNDSSSTAYWAGF
                     TLDWYRQLFNDRVIFESFKVSIAVALLTSVLSALIGTASAIISVYVTKKTEKAMNGLM
                     FLPLIVPEVALGVSLLIFFSELKLPFGIFTLVISHSLFCVPYVHIMVLLRLKEINISI
                     IEAAKDLGATRWQLIKTILLPLVMPSTLTASLLALAMSLDDVIISTYMAGPTTTTLPV
                     HIFSMMRVGVTPKVNALCTLFLAGTFLLIGLNQIVGTKGGNVVEQKQIR"
     gene            4123..5193
                     /locus_tag="PKK75_07210"
     CDS             4123..5193
                     /locus_tag="PKK75_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ83425.1"
                     /translation="MSKSKSGKLSVLLIIALLTAVFTGCGKETGTVEQINLMSWGGDF
                     IPRQVITEFEEETGIKVNYKEVTSNEDMQSLLEANPDQYDLAVVTDYMVDILRLNGNI
                     KVLDKGKLPNFSNINPVYQGNYYDENNEYSMPYAISTSLLLVNPEAVEELGADPITGY
                     KDLWQEELKDKVVIVDWSVEVIGVVLKALGYEYNETDPDIVAEAREKLFELAPNIVRF
                     ETNTPEDSLINGEAVAGFMYSNQAVKGQAADSSLEPVFPVEGMPIFIDSFVMSKEAPN
                     EDNALKFLDFLLRPEIAARIADITNFTTPNKAAEEFVSEDYKNNPMLNIPDEVAANTS
                     FYIDVATVLEEYDHIYSEFKMQ"
BASE COUNT         1645 a          872 c         1203 g         1548 t
ORIGIN      
        1 ggggttgatg catatggagt ttcagcagat ccgagactat atgcaggaca gaagttcagg
       61 gaagcaaggg agattgctgc cagagcaaaa gattttgtta aatgtatttt caaaccgcct
      121 gcggctattt taggagaaac gatacccgta agcggcgacg gaaatgttac caatgataaa
      181 tattgatact ttatttatgc tgtattaaat ctcttgacaa taaatttata ttggattagc
      241 ctttatgtag ggaaagaata attttccaaa ttaagctggg gggaaaagtt tatgtaccat
      301 gcaagattta aaggaaggca ttatgaagca ggcttaaaat ggggaaagct gttattaaaa
      361 aacggaataa agcttgattt cagccccaca tttgaactta ctgaagaaag gtttagattt
      421 gcagaaaagt gtttggatga atacaagaga tattttccgg agatacttga cgaaataagg
      481 ggtattgcag atggcaacga ggttcccgaa aatgtactcc agactatttt gtttagtatg
      541 tattgcttcg aacttgataa cagatgcaca gtctttgcaa tcagcacgga agatgaaatt
      601 attttcgggc ggaacagtga ttttttaaaa agtcttgaga agctgtatat gaattgtatt
      661 tacaggctga cgggaagcaa ttctttcaat gccaacacca ctgcctatgt gcagatggag
      721 gatggaataa acgaacacgg cctggctacc ggacttacat ttgtatatcc tagaattagg
      781 aaacctggac tgaatgcagg gatgcttgtc agatatctgc tggaaaaatg cagtacaaca
      841 aaagagtctg ttgaggaact gcaccgcctt cccatagcat cagcccagac aataacatta
      901 gcagacaaaa aaggtgatat tgcagtggtt gaatgcaatc ctgaaaatat tgttgtcata
      961 gaacccgagc ccggcgaaca atttgttgca acagcaaata atttctattc tgtgcagatg
     1021 agaggctggc gcaacgggga tattgatgac tggaggtcgg atgaacggta tcaaacagca
     1081 tttagtgcat tgaaaaataa taaaggcaat tattcggttg accttgccaa atccatcttg
     1141 tcggggaaat atggatttat gtgtcaatat gacaggaagg gaattgcaga taccgtgtgg
     1201 tctgtagtat atgatattaa gcacgataga atatggaggg ttgagggaaa tccttcaagg
     1261 aaaaagttca gggagatatg ataaagttca gcccataaaa ggtttgtaaa tttttagaaa
     1321 atgttataat atagtagtcg cctgtaaagt tcataggctc aattatataa aatggacaag
     1381 gatagtatat gaaggaacaa ttactcagca taagaaaaat taataaatat tttggaaggt
     1441 cacatatatt aaaggatgtg acattagaaa taaaagatgg agaatttata actcttttag
     1501 ggccgtcagg ctgcggaaaa accacaatac tcaggtgcat tgcagggctt gaatctattg
     1561 attcgggaga tattttgcta tacggtgaat caatcaagga tttagcacca aataagcgaa
     1621 atgttaatac tgtatttcaa aactacgccc tgtttcctca tctgaatgta tatgacaata
     1681 ttgcatacgg gcccagaata aaaaagtcca taggggaagg cgagatcaaa aaaagcgttg
     1741 ctgaaatgct ggaattagtt caaatgcagg gctatgaaaa gagaatgacc caccagctgt
     1801 ccggcggtca aaggcagaga attgccattg ccagagcctt gattaacaag ccggatattt
     1861 tgcttttgga tgaacccctg ggagccctgg atttaaagct caggaaatat atgcagacag
     1921 agcttgccca ccttcaaaaa caagctgaaa caacttttat atatgtaaca catgatcagg
     1981 aggaagcatt aaatatgtct gatcgtatag tggttataaa tgaaggtgtg gttcagcagg
     2041 taggcacccc aagcgatata tacaataatt ccaaaaacct ttttgttgca ggttttatag
     2101 gggacaggaa tattaaagag gttaaggtcc ttcaaaagaa gggggacttt tgcagtgtga
     2161 aattaggcaa tagcgttatt tacataaaac ctgacagaag cctggtggac cacgataaat
     2221 gcagtgaaat ggaagcagct gtactggctg tacacatgga taaaatgaaa atatcctatg
     2281 aggaggtccc aaatagtctc tacggacaga taacatttgt ccattatgca ggctctcaaa
     2341 ttaaaactga ggtggatgtg gacggggaac ttttgacagt tattgaatac caaaatgata
     2401 atgacaatta tagtataggg gataaggtat atgtttcctg ggaagaaacc ggagtcttgc
     2461 ttcttcctaa agaagataca acagggagga gagaagatga aaaggattaa acaaaatttg
     2521 cctgcactcc ctatactggg gtatttatta ttgctggttg gcattcctct tttgctggtt
     2581 gttattttaa gttttttatc aagggacagc ctgggcaata ttatttttga atttacaccg
     2641 gataactaca ggaagatgtt tgatcctgtt tacttgaggg tttttctcaa ttcattttca
     2701 ctggcattat ttaccggatt aattacactt ctggttgggt acccggttgc atattttacg
     2761 gcaggtcttg aacaaaaaag gcggtctttt gttattttgc tgattatgct gcccttttgg
     2821 atttcaagct tattgaggac ttacgggtgg accatacttc tgggaaaaga agggcttatt
     2881 aataatgttc ttatgttttt taacattatt gaccagccct tgaacatgat gtataaattc
     2941 ggaacggtat tgactggtac ggcatatatg ctggtgccct ttatgataat ttccatatat
     3001 aattctgttg ataagcttga caggtctttg ctggaggctt cttatgacct tgggggcggc
     3061 aagtggaaga cattttttaa tataaccctt cctcttacaa cgccgggaat agccaggggc
     3121 tttacacttg tttttattcc tgctctggga ctttattata tatcagacct cctgggtgga
     3181 gggaacacca tgtttttggg aaacttgata aataatatgg ctacaagggg tagaaacagg
     3241 cctttggcat ctgcatttgc cgtggggatg attctcctgg ttttattggt cctgtttata
     3301 tacggcagag tgacaaagag cggtaaggag agtctatatc aatgaatata aagcaaaaaa
     3361 aattcagaac attttatttg tcattatttt tcataatatt gtatgttccg gtgctgagtg
     3421 ttgtattgta ttcatttaat gatagcagct ccactgcata ctgggcgggc tttacccttg
     3481 actggtacag gcagcttttc aatgacaggg ttatattcga atccttcaaa gtctctattg
     3541 ctgttgcctt gttgacttcc gtcttatctg ccctaatagg gacggcttct gcaatcatat
     3601 ccgtatatgt aacaaaaaag acggaaaaag ccatgaacgg attgatgttt ttacccttaa
     3661 tagttcctga ggtagccctg ggggtatcac ttttaatatt tttcagtgaa ctgaagctgc
     3721 ccttcgggat ttttacgctg gttatctccc actcgttatt ttgtgtgccc tatgtgcata
     3781 ttatggttct gttgaggctg aaggagatta atatatcaat tattgaagct gccaaggatt
     3841 taggcgctac cagatggcag cttattaaaa ccattctgct gcccctggtt atgccttcta
     3901 ctctgacagc atctctttta gcacttgcca tgtccctgga tgatgtgata atatcgactt
     3961 acatggcagg acctactact acaacattgc cggtgcatat attttccatg atgcgggtag
     4021 gagttacccc aaaggtaaat gccctgtgta cattgttttt agcaggaaca tttttattga
     4081 taggtttaaa tcaaatagtc ggaacaaaag gaggaaacgt agttgagcaa aagcaaatca
     4141 ggtaaattat cagttttatt aataatagct ttattgacgg ctgtgtttac agggtgcggc
     4201 aaagaaacag gcacagttga gcaaatcaat cttatgtcat gggggggtga ttttataccc
     4261 cgccaagtga ttacagaatt cgaagaggaa accggtatca aggtcaatta caaggaagtt
     4321 acgtctaatg aagatatgca gtctttattg gaggcaaatc ctgaccagta tgatttggct
     4381 gtagtgacag attatatggt ggacatctta aggctgaatg gaaacattaa agttttggat
     4441 aagggaaaac tgccgaattt ttctaacata aaccctgttt atcaaggcaa ctattatgat
     4501 gagaacaatg agtattcaat gccctatgcc atttccacat ctcttttgct tgtgaatccg
     4561 gaagcagttg aagaattagg cgcagatcct attactggct ataaggattt atggcaggaa
     4621 gaattaaagg ataaggtagt tatagtagac tggtctgttg aagtaatcgg agttgttttg
     4681 aaagcattgg gatacgaata taatgaaaca gacccagata tagttgctga agctagggaa
     4741 aaattatttg agctggcacc aaatattgtg cggtttgaaa caaatacgcc tgaagactcg
     4801 ctgataaacg gcgaagcagt tgcaggattc atgtattcaa atcaggcagt aaaaggccag
     4861 gcagctgatt ccagccttga gcccgtgttc cctgtggaag gaatgcctat atttattgac
     4921 tcctttgtaa tgtcaaagga agctcccaat gaagataatg cattaaaatt cctggacttc
     4981 ctgcttagac ctgagattgc tgccaggatt gctgacataa ccaatttcac aacaccgaat
     5041 aaagcagccg aagagtttgt atcggaagat tacaaaaaca atcctatgtt gaatattccc
     5101 gatgaagtgg ctgcaaacac aagtttctac atagatgtgg ctactgtttt ggaagaatat
     5161 gaccatattt attctgaatt taaaatgcaa taggtaatac agaatggaga aaagtgggcg
     5221 gctaaaattt tagccgccta attattataa atgtgtgggg acattcct
//
LOCUS       DAOPUT010000093         5226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_15869_length_5226_cov_19.219107, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000093 DAOPUT010000000
VERSION     DAOPUT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15869_length_5226_cov_19.219107"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PKK75_07215"
     CDS             complement(<1..928)
                     /locus_tag="PKK75_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ83426.1"
                     /translation="MVSFETLRNELIGKDFKFETPYGERLLTYADFTASARGLNFIEK
                     YMLEIQKSYANTHTEDDMTGEVMTKILHKSEEIIKAHVNGGDNCYVIPSGTGATGAIE
                     CLAKILGIYIPPAAKRTVDNITKEYYESNKGIDEIIDLKSYQKYQPLVFIGPYEHHSN
                     ILMWEEGIAEVVEIGLKSDGTIDLDDLKRTLSNQKHKDRLKIGSFSAASNVTGIITPV
                     YEIAEILHENNAYACFDFAACAPYMEINMNKSKTEYFDAVYLAPHKFVGGPGSSGILV
                     INKRVYDNSLPPTVSGGGTVSYVSPYAYDYIDN"
     gene            complement(1197..2498)
                     /gene="lysA"
                     /locus_tag="PKK75_07220"
     CDS             complement(1197..2498)
                     /gene="lysA"
                     /locus_tag="PKK75_07220"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNZ83427.1"
                     /translation="MVVLEIRGNILYFDGCNTVELAKKYGTPLYVMSETKIADKCKEI
                     RDTFLNRYERTRAAYASKALLTLAMCKLIEREGLCLDVVSGGELYTAIKADFPPERIE
                     FNGNNKSIEELELAVDYDIGRIIVDGFDELEIIEDICRKKGKKTNILYRITPGVKSDS
                     HDYIVTGKKDSKFGFPLDYDVIFPAIEKAIKSQLVKFLGFHFHVGSQLHDNESHLKAL
                     DVALKLIKDTIDEYDYVISELNVGGGFGIRYTDADDKKPYAYYLDPMMEKIEEFSKRM
                     NIRRPEIVIEPGRSIVGEAGITLYTVGTIKDIKGIRKYVSVDGGMTDNIRPALYQAKY
                     KGLIANKADQPPDDLVTISGKCCESGDILIKDAYLPLAERGDILAIFSTGAYGYAMAS
                     NYNKNPFPATVLVRDGKSEIIVKRQTYEHMIANEVIPESLL"
     gene            complement(2675..3043)
                     /locus_tag="PKK75_07225"
     CDS             complement(2675..3043)
                     /locus_tag="PKK75_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNZ83428.1"
                     /translation="MASIEEVQLILDEIAEELPPEIYKDLNGGIILLPQIKLHKKSVD
                     DDLYVMGEYRHEMISGRYIVVYYGSVEKLFGNLSRNRLKNKLRSVIKHEFIHHLESMA
                     GERHLEIEDERKLAEYLKKK"
     gene            complement(3710..>3952)
                     /locus_tag="PKK75_07230"
     CDS             complement(3710..>3952)
                     /locus_tag="PKK75_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ83429.1"
                     /translation="RPDIIISDIGLPDMSGHEVAAEIRKLTEFKDTIMIAMSGYAQTE
                     DIMRSKEAGFDRHLCKPVSIDTLTRVLDELSNRINR"
     assembly_gap    3953..4052
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4053..>5226)
                     /locus_tag="PKK75_07235"
     CDS             complement(<4053..>5226)
                     /locus_tag="PKK75_07235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNZ83430.1"
                     /translation="ARYDEAIKTGKSISFEMYGRAFDQYFRMLVIPLGEQNRFGIMYQ
                     DITELHNKNLDLKESEAKYQRLFETSNDGYSLTDKDGYIIDANNSSSNMLGYYKEEVI
                     GRLWTEFVDDEYLDKTYNEWEFRRSGKSGRYQVKLKRKDKSSIWALISASPIMDEKGD
                     YNGTLLAYKDINEQKTAEEKLIKSEQNAFALVARLEEANKNMTHFISVLSHELRNPMA
                     VIMLNLELQKMASNKEERAIKTFENIERQTEYLNHMINDLLEVTRITNKRIVLKKEQV
                     DLNLLAADVLKDYQEPYDEKEIGLELINSPNPIIIEADSVRMNQIISNLLNNAAKFTP
                     KGGKVTVNINIDEINKQAIICVQDDGEGISPEFLPDLFEPFAQADRTIDRKHGGLGL"
BASE COUNT         1455 a         1092 c          759 g         1820 t
ORIGIN      
        1 cattatctat ataatcatag gcatagggac tcacatagct tactgtcccg ccgcctgata
       61 ctgtcggggg aagagaattg tcatataccc ttttattgat aactagtata cctgaagatc
      121 ccgggcctcc cacgaattta tgtggagcca aatatacagc gtcaaaatac tccgtcttac
      181 ttttattcat atttatttcc atataggggg cacatgcagc aaagtcaaaa caagcatagg
      241 cattgttctc gtgaaggatt tctgctatct catacaccgg tgtaattatt cccgttacat
      301 ttgaagctgc cgaaaaggat cctatcttta atctgtcttt gtgcttttga ttagataatg
      361 ttcttttcag gtcatccaaa tcaatagttc catctgattt aaggccaatt tccactacct
      421 ccgctatacc ttcctcccac ataagtatgt ttgaatggtg ctcgtagggc cctataaata
      481 caagaggctg gtatttttga taacttttta aatctattat ttcatcaata cctttattgc
      541 tttcataata ttccttagta atattatcta cagttctttt ggcggcggga ggaatatata
      601 tgcccaaaat ctttgcaagg cattctatcg ctcctgtagc acctgtgcct gaaggaatta
      661 cataacagtt atctccccca tttacatgag ctttgattat ttcttccgac ttgtgcagaa
      721 ttttagtcat gacctctccg gtcatgtcat cttctgtatg tgtatttgca taggactttt
      781 gtatttcaag catgtatttt tcaataaaat tcaagcccct tgctgaagct gtaaaatcag
      841 cataggtaag cagtctttcc ccgtatggag tttcaaattt aaaatctttt ccgattaatt
      901 catttctcaa tgtttcaaaa gaaaccataa attacatcct tttcttgaat taattttatt
      961 ataaccaaaa aacaaaaaaa caacggaaaa aaccgtctca agaatcttgt acctgaaatt
     1021 attgtttaca taaattaaca tatgtaatcc aaattgcgat aacccagtct ctttgtcaca
     1081 gtgatgatta aaattctagg ctacataaat tcatatatgt aaactaaata aaaatatccc
     1141 catcccgatg aaactgcaag cttaaatcat gggaacaaaa agaccgttcc caaaacttac
     1201 agcaatgatt ccggtataac ttcatttgca atcatatgct catatgtctg cctttttaca
     1261 ataatttctg atttgccatc ccttaccagg actgttgctg gaaatggatt tttattgtag
     1321 ttgcttgcca tggcgtaacc gtatgctcct gttgaaaata tggcaagtat atctcccctt
     1381 tcagctaaag gcagataagc atcctttatt aatatatccc ccgattcaca gcatttcccg
     1441 ctaatggtta ctaaatcatc gggcggctgg tctgctttat ttgcaataag ccccttatac
     1501 ttggcctggt ataatgcagg ccttatattg tcagtcatac cgccgtcaac agacacatac
     1561 tttcttattc ccttgatatc cttaatggta ccgactgtat aaagtgttat cccggcttct
     1621 ccaactatgc ttcttcccgg ttcaattaca atttcaggcc tcctgatatt catcctcttt
     1681 gagaattctt ctattttctc catcattgga tctaaataat acgcgtaggg ttttttatca
     1741 tcagcatctg tatatctgat tccaaacccg ccgcctacat ttaattcaga tattacataa
     1801 tcatattcat ctattgtgtc ctttatcagc tttaatgcaa catccaatgc ttttaaatgt
     1861 gattcgttgt cgtgaagctg ggaacccacg tggaaatgaa aacccaggaa ttttacaagc
     1921 tgtgacttta ttgctttttc aattgccgga aatataacat cataatccaa ggggaatcca
     1981 aactttgaat ccttttttcc tgtaacaata taatcatgag aatcactttt aacacccggt
     2041 gttattctgt aaaggatgtt tgttttcttg cccttttttc tgcagatatc ttcgattatt
     2101 tccaattcat caaagccgtc cactattatt cttccgatat cgtaatctac agcaagctct
     2161 aattcttcga ttgatttatt attaccgttg aattcaatcc tttccggagg aaaatcggct
     2221 tttatggctg tatacagctc gcctcccgaa accacatcca aacaaagacc ttctctttca
     2281 attaacttgc acatggcaag tgttaacaat gcttttgaag cataagcggc ccttgttctt
     2341 tcatacctgt ttaaaaatgt atctcttatt tctttgcact tgtctgcaat ttttgtttca
     2401 ctcatcacat acagcggtgt tccgtacttc tttgccaatt caactgtatt gcatccatca
     2461 aagtacaata tgttacccct aatctcaagc acaactattt ctcctttata agctaatatt
     2521 atcaattaat aaattataac gcattatata aaaaattatg caaacaatct caaacaaaca
     2581 cctagcgaaa ttatagtgtt attatttaaa tagtacaatt atccaagtcc aatagaattc
     2641 ttataataac ttgtttttca tgctgcatgt cccgttattt ctttttcagg tattctgcta
     2701 atttgcgctc atcttctatt tccagatgtc tttctcctgc cattgattca aggtgatgga
     2761 taaattcatg ttttataacc gacctgagtt tatttttaag cctgtttctt gaaagattgc
     2821 caaacagctt ttcaactgat ccatagtata caacaatata ccttcctgaa atcatttcat
     2881 gtcggtattc acccattaca taaaggtcat catccacact ttttttatgc agcttgattt
     2941 gaggcagcaa aataatgccc ccgttaaggt ccttgtaaat ttcaggaggc agctcctctg
     3001 ctatttcatc caatataagt tgtacttctt ctattgatgc cataattaat cctttctaag
     3061 caatacctaa gagcagcatt ttccattaaa acaataccgg gaataccgat tttgttaatg
     3121 gcatggctat ccatttcctt catttcctgg cagcttattg attccaaatt aaattcctcc
     3181 atttttatta tataatagca tcggtgagca gctttgtcaa aagatgtaaa ttcatattat
     3241 ataggttaaa attaattgac taaagaggaa gttgaaatat agtaaaaatc aagatataaa
     3301 aagactaagc cggaaaatca tacttccggc ttattagaat tatctgtttt tgaagtgaat
     3361 acccgtttaa acaggttata tatgcccgct cctgccaaca gcagtatgta agttaaaact
     3421 cagggaggac aggcatagag cttgactttt tttgaattat tcatcattgc ttcctttctt
     3481 tataggcatc gatacaaatt gaaacaaacc ttagtatgcc gcagtttgcc ttacacaccc
     3541 cttccatgcg tgctccgggg tgacacctgc atcatgcatt gatatcccaa catttatagc
     3601 ttttatattg gtgttagttt ataagctcct gatattgtat ttttagtaaa ttttattata
     3661 ttttacaaac attagtttaa ccttaaattt tcttaaatat ttctcaacac tacctattaa
     3721 tcctgttact caattcatcc aatacccttg ttaatgtatc tatacttaca ggtttacaaa
     3781 gatgccggtc aaatccggct tcttttgaac gcatgatatc ttcggtctga gcataacctg
     3841 acatggcaat catgatagtg tctttaaact cagtcaattt acggatttcc gcagccacct
     3901 catgcccgct catatcaggc agtcctatgt ctgatatgat tatatccggt ctnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagtccgag acctccatgc ttcctgtcaa
     4081 tggttctatc tgcctgagca aatggttcga ataaatctgg caggaactca ggcgaaattc
     4141 cttcaccgtc atcttgaacg catataattg cttgtttatt tatttcatct atattaatgt
     4201 ttactgtcac tttcccacct ttaggtgtga acttagcagc attattcagc aagttactga
     4261 taatctggtt catacgaacc gagtctgctt ctattattat agggttggga ctgtttatga
     4321 gttccagccc tatctccttt tcatcatacg gttcctgata atccttcaat acatctgctg
     4381 ccaataaatt caaatctact tgctcttttt ttaacacaat ccttttattt gtaattcggg
     4441 taacttccag cagatcatta atcatatgat tgagatattc cgtctgcctc tctatgtttt
     4501 caaatgtttt tatagcacgc tcttccttat ttgatgccat cttttgcagt tctaaattca
     4561 acataataac agccatcgga tttctcagct catgggataa aacactaata aaatgagtca
     4621 tgtttttatt ggcttcttca agccgggcaa caagagcaaa tgcattttgc tcgcttttga
     4681 ttaatttttc ttctgccgtc ttttgttcat ttatatcttt gtatgccaat aaagtgccgt
     4741 tatagtcacc tttttcatcc attattggcg atgcgcttat aagagcccaa attgagcttt
     4801 tatctttcct tttaagttta acttgataac ggcctgattt tcctgacctt cgaaattccc
     4861 attcattgta tgttttatct aaatattcat catcaacgaa ctctgtccaa agcctgccga
     4921 ttacctcttc tttatagtat ccaagcatat tagatgacga attgttggca tcaattatat
     4981 atccgtcttt atcagtcaat gaataaccgt cattagatgt ttcaaaaagt ctttggtact
     5041 tagcttcact ctctttcaag tcaaggttct tattgtgtaa ttctgttata tcctgataca
     5101 taattccaaa cctgttttgc tcccccagtg gaattacaag cattctaaaa tattggtcaa
     5161 atgccctgcc gtacatttca aaagagattg acttgccggt tttaatggct tcatcatatc
     5221 gtgcaa
//
LOCUS       DAOPUT010000094         5180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_16072_length_5180_cov_19.743415, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000094 DAOPUT010000000
VERSION     DAOPUT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16072_length_5180_cov_19.743415"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PKK75_07240"
     CDS             <1..161
                     /locus_tag="PKK75_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ83431.1"
                     /translation="WCFEKCRNLKIDTHRDNIPMQNLLNKNGFIRCGIIYLENGDERI
                     AYQKADMV"
     regulatory      323..427
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            562..1833
                     /locus_tag="PKK75_07245"
     CDS             562..1833
                     /locus_tag="PKK75_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNZ83432.1"
                     /translation="MKNDFKLETALVQSLEDFQEGEPRVYPLVQSTTYNYKDPDFVAG
                     LFDLTETGHMYSRISNPTVQAFENKMAQLEGGVGAVAVSSGMAATTMAITNICNLGDH
                     VVALSTIYGGTFTLLSSSLKKLGIETSFFSPDSTEEEILSLVQDNTKLIFGETIGNPG
                     MNVLDFEKISNAAKKAGLPFIVDNTIATPYLCRPIEHGADIVVHSTTKYIDGQATCMG
                     GVVIDGGNFKWDNGRFPGLVEPDPSYHGLSYYETFKEKAYITKARTNILRDYGAVLSP
                     FNAFINLRGLETLHVRMDRHCENALKVAEFLEGHENVLWVNYPKLKSGKYYENAEKYL
                     PRGGSGMILFGIKGGAKAGREFTKKLKMINNVVHLGDVRTSVLHPGSTTHRQLTDEEQ
                     IAAGVMPEAIRLNVGIENIEDIINDIKSALE"
     gene            1856..2791
                     /gene="metA"
                     /locus_tag="PKK75_07250"
     CDS             1856..2791
                     /gene="metA"
                     /locus_tag="PKK75_07250"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNZ83433.1"
                     /translation="MPVIIPIDLPAYKTMEDENIFVMSRERAETQDIRPLEIALVNLM
                     PTKIDTETQFVRLLSNSPIQINVEFIHTESYKAKNVVQSHMKSFYKCIDEVKDKKYDG
                     MIITGAPVEHLAFEEVDYWEELKQILEFSHRNVTSAMYICWGAQAALNYFYGLNKREL
                     PAKVFGVFEHEKKHATCMLLNGFDDVFYVPHSRHTEVNKEDIVSVEELIILAESEEAG
                     VHIAVTDDYSRIFIMGHSEYDPLTLKREYDRDISQNKPINIPKNYYPGDDPSKPPFVK
                     WRSHANLLFKNWINIIYQETPYDISKVGTKNRVFF"
     gene            2893..3840
                     /locus_tag="PKK75_07255"
     CDS             2893..3840
                     /locus_tag="PKK75_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNZ83434.1"
                     /translation="MKTKLTDLLHIKYPILQGAMAWISEANLAAAVSNAGGAGIISTG
                     GRDLSWVKEQIELARTLTDKPFGLNVVLMDEDKDEKIDLICSEKVSFVTLGAGNPVPY
                     IEKLKNAGIKVIPVVPNTKLAKRVEENGADALIIEGMEAGGHIGKITTLALMTQVIPE
                     IKIPVIAAGGFADGRGLAAAIVMGAAGIQAGTRFYASKECRAHINAKNAIVAAGDMGT
                     EITGYLANHSIRSLKNKATDKYIELEKANAPSEELSKLRAGKAKSAPFEGDVEWGLVQ
                     AGQSLSVITSIKSCEEIINDFIREADEALRRVQGIEFAR"
     gene            3890..4732
                     /locus_tag="PKK75_07260"
     CDS             3890..4732
                     /locus_tag="PKK75_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase"
                     /protein_id="HNZ83435.1"
                     /translation="MDLINKQVTHKSFGKGSIVDCNDAYVVVKFKSGNKEFVYPDAFK
                     KYLTLIDKRAADSVDKILEKLEMEREIEEQRIEEKKAIEHEEQQRLLRIEKLKKHKIH
                     SSSQVAFNCREEELDAVFSDWKIFTGSVKSGDNEGRTNKLIRVLQNSACLVTSKDKGA
                     PEKERYIKGLFMVDEGFAGRLCEDGYIPAHPYYRIRLTEKESKRMPFWKYYTNDRYPD
                     NITWSGRSRYFSNAVMARILKDILSFRSEQEDYTQEIFDYFCLLNQIDEKEIPLPEGP
                     LTDK"
     gene            4755..>5180
                     /locus_tag="PKK75_07265"
     CDS             4755..>5180
                     /locus_tag="PKK75_07265"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNZ83436.1"
                     /translation="MKYPKPTIFVSHIHENKEMALVIKQLIERIFENALNIFVSSDSE
                     SIDLGDDWLMNINEGLKECSIMLIICSPESINRPWVAFEAGVGWSKGIKVVPLCCFGL
                     TISSLPMPYKRWQAANANREDSLNRLMKIIAREFDFKYNE"
BASE COUNT         1820 a          829 c         1198 g         1333 t
ORIGIN      
        1 attggtgctt tgagaagtgc aggaatttga agatcgatac ccaccgtgac aatattccca
       61 tgcaaaattt attaaacaaa aacggtttta tacgatgcgg aataatttac ctggaaaacg
      121 gagatgaaag aatagcatat cagaaagctg acatggtgta atatttacaa aacaaaattt
      181 ataaaggaca taaaaaactt gtaaaaaatc atggcgaacc tgtaaagaaa atacagaact
      241 aacaaaaaga cataggaatc atgtaaaaaa accacttgac aaaaaaatat atatggttta
      301 aattaaataa caattaaata aattctcatc aagagagacg gagggaacgg cccgatgaag
      361 tctcggcaac ctacattcat taatgtaagg tgctaattcc ggcaaagatg gctttgaaag
      421 atgaggagac ggcaaacaat taattttcct ctcttcatta gaggaatttt tatttgcggc
      481 agattccgag tttatggagt ggaataagca aataaaatcg gtggataaga ctaggactta
      541 tataatatta aggaggaaaa catgaagaat gattttaaat tagaaacagc attggtacaa
      601 tcattggaag attttcagga aggtgagcca agagtctacc ccttggtgca aagtactaca
      661 tacaattaca aggatcctga ttttgttgca ggtttgtttg atttaactga gacaggacac
      721 atgtattcaa gaataagcaa ccctacagta caggccttcg aaaacaaaat ggcccagctt
      781 gaaggaggag taggagctgt tgcagtttct tcaggcatgg cagccacaac aatggcaata
      841 acaaacattt gcaatttagg agatcatgta gttgccttat caactattta cgggggaaca
      901 tttactctgt tgtcatcaag cttgaaaaag ttgggtattg aaacatcgtt ttttagccct
      961 gacagcactg aagaagaaat attatccctg gttcaagata ataccaaatt aatattcggt
     1021 gaaacaattg gcaatccggg gatgaatgta ttggactttg aaaagatttc caatgcagcc
     1081 aaaaaagcag ggcttccctt tatagttgac aatacaattg ctactccgta tctttgcagg
     1141 cctatagagc atggtgcgga catagtcgta cattcaacca ccaaatatat tgacggccag
     1201 gccacatgca tgggtggtgt tgtaatagac ggagggaatt tcaaatggga taacggaagg
     1261 ttccccggac ttgtagagcc tgaccccagc tatcatggac tgagctacta tgaaacattt
     1321 aaggagaagg catatattac aaaggcaaga accaacattt tgagggatta cggggcagtt
     1381 ttaagtccct ttaatgcatt tattaattta agaggccttg agacactcca tgtgagaatg
     1441 gacagacact gtgaaaatgc tctgaaggtg gcggagttcc ttgaggggca tgaaaatgta
     1501 ttgtgggtta attatcctaa gctaaaaagc ggcaagtact atgaaaatgc cgaaaaatat
     1561 ctgccaaggg gtggcagcgg aatgatttta ttcggtataa aaggcggagc aaaagcagga
     1621 agggaattca ccaagaaatt aaagatgatt aacaatgttg tccatctggg ggatgtcagg
     1681 accagcgtgc ttcatcccgg cagcaccact cacaggcagc tgactgatga agaacagatt
     1741 gcagcaggtg taatgccgga ggcaataaga cttaatgtgg gaatagaaaa tattgaagat
     1801 ataataaatg atattaaaag tgcattggaa taaaacctgg gaggatgaca aaaaaatgcc
     1861 ggttataata cctatagatt tacctgctta caaaacaatg gaagatgaaa atatttttgt
     1921 aatgagcagg gaaagagctg aaacacagga catcaggcca ttggagattg cactggtcaa
     1981 tttgatgccc acaaaaatag ataccgaaac acagtttgta agactgcttt caaacagccc
     2041 gattcaaata aatgtggagt ttattcatac tgaatcctat aaggctaaaa acgttgtaca
     2101 aagccatatg aaaagctttt ataaatgcat tgatgaagtt aaggataaaa agtatgacgg
     2161 aatgattatt acaggggccc cggtggagca tcttgcattt gaggaagtgg attattggga
     2221 ggagttgaaa caaatcttag aattttccca cagaaatgta acatctgcaa tgtatatatg
     2281 ctggggagcc caggcagcgc ttaattactt ttacggacta aataaaagag agctgccggc
     2341 aaaggtattc ggcgtgtttg aacatgaaaa aaaacatgca acatgcatgc tgctgaatgg
     2401 ttttgacgat gtgttctacg ttcctcattc aagacatact gaggttaata aagaagatat
     2461 tgtttcagtg gaagagctga ttatactggc ggaatctgaa gaagcgggag ttcacattgc
     2521 agttacggat gattacagcc gcatattcat catggggcat tcggaatatg atcccttaac
     2581 cttaaaaagg gaatatgaca gggatataag ccagaacaag ccaattaata tccccaagaa
     2641 ctactatccg ggggatgatc cttcgaaacc gccctttgta aagtggagaa gccacgcgaa
     2701 tctgctcttt aaaaactgga taaatataat ttaccaggaa acaccttatg atataagtaa
     2761 agtgggaaca aaaaacagag ttttcttcta gaatttaaga aagccgcctc atattttcca
     2821 ggcggctttt ttatgtggta atttacgtaa taaactggta taatatagtt agaatgaaca
     2881 aggaggaata ccgtgaaaac taaattaaca gacttattac atataaaata tccaatactg
     2941 caaggcgcaa tggcatggat atctgaagcc aatcttgctg cagccgtttc gaatgcagga
     3001 ggtgcaggaa tcatttctac agggggaaga gatttatcct gggttaagga gcagattgag
     3061 cttgcaagaa cattgacaga caagcctttt gggcttaatg tggtattgat ggacgaggac
     3121 aaggatgaaa aaatcgattt aatctgcagt gaaaaagtgt cctttgtaac cttgggggca
     3181 gggaatccag ttccctatat tgaaaaacta aaaaatgccg gtataaaggt aattcctgtt
     3241 gtgcctaata cgaaattggc caagcgggtt gaagaaaacg gagcggatgc tttgataata
     3301 gaaggcatgg aagcgggagg ccatataggg aaaataacaa ccttagcctt aatgactcag
     3361 gttattccgg aaattaaaat tcctgttatt gcggcaggag gttttgcaga tggcagaggc
     3421 ttagctgcag caatagtgat gggagctgca ggaattcaag caggaacaag gttttatgct
     3481 tcaaaggaat gccgtgctca tataaatgcc aaaaatgcca ttgtcgctgc aggagacatg
     3541 ggaaccgaaa taacaggcta tcttgccaat cattcaataa gaagtcttaa gaacaaggct
     3601 acggacaagt atatcgagtt ggaaaaggca aatgcaccct ctgaagaact gagtaaatta
     3661 agggcaggaa aagcaaaaag cgcacctttc gaaggagatg ttgaatgggg actggttcag
     3721 gcagggcaaa gcctgtcggt gataacttca ataaaaagct gtgaagaaat tataaatgat
     3781 tttatcagag aagctgatga agcattaaga agggtgcaag gaatagaatt tgcaagatag
     3841 tttaaattgt gatactatgt atatagaatt catataaggt ggatgtagaa tggatttaat
     3901 taataagcag gtaacgcata aaagctttgg caagggaagc attgttgatt gcaatgatgc
     3961 ttatgtggtc gttaagttta aatctggaaa caaggagttt gtttatccgg atgcgtttaa
     4021 gaaatatctg acattaattg ataaaagagc agctgattca gttgataaaa ttcttgaaaa
     4081 actagaaatg gaacgggaaa ttgaagaaca gagaattgaa gaaaagaaag caattgagca
     4141 tgaagagcag cagcgtttat taagaataga aaaactaaaa aagcacaaaa ttcacagctc
     4201 ctctcaggtt gctttcaact gcagggaaga ggaacttgat gctgtatttt ccgattggaa
     4261 gatatttacc ggaagtgtaa aaagcggtga caatgaaggc aggacaaaca aattgatccg
     4321 ggttcttcaa aacagtgcat gccttgtaac gtcaaaggat aaaggtgcac ctgaaaagga
     4381 aagatatata aaaggactgt ttatggtgga tgaaggtttt gcaggaaggc tgtgcgaaga
     4441 cggctatata ccggctcatc cctattacag aatcaggctt actgaaaagg aatcaaagag
     4501 gatgcccttt tggaagtact atacaaatga cagatatccg gacaatatta catggtcagg
     4561 cagatcacgc tacttttcca atgcagtgat ggcccggatt ctcaaggata ttttatcatt
     4621 tagaagtgag caggaagact atacccagga aatttttgac tatttctgtc ttttaaatca
     4681 gatagatgaa aaggaaatac cattgccgga gggcccgttg acggataaat aattaattgt
     4741 atcaggggga acctatgaaa tatcctaaac ctacaatctt tgtatcccat attcatgaga
     4801 ataaggaaat ggctttggtt atcaaacagt taattgaaag gattttcgaa aatgcattaa
     4861 atatatttgt ttcatcagat tcagaaagca tagacctggg cgatgactgg ctgatgaata
     4921 tcaatgaagg attgaaggaa tgctctatta tgcttatcat atgcagtccg gaatcaatca
     4981 atcgtccctg ggttgcattt gaagcaggcg taggatggtc gaagggaata aaagttgttc
     5041 ccttgtgctg ttttgggtta actataagca gccttcccat gccctataaa cgatggcagg
     5101 cagctaatgc aaacagggaa gactctttaa accggcttat gaaaatcata gccagggagt
     5161 ttgatttcaa atacaatgaa
//
LOCUS       DAOPUT010000095         5084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_16486_length_5084_cov_19.550010, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000095 DAOPUT010000000
VERSION     DAOPUT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16486_length_5084_cov_19.550010"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1899
                     /locus_tag="PKK75_07270"
     CDS             97..1899
                     /locus_tag="PKK75_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ83437.1"
                     /translation="MSYLRLIEDKIQSFAESMSSTLNVEITVVDNNLMRIAGTGDFYY
                     RINMTSPSNSWFARVLKTGIEVMNLNKGSNSVCAKCVDLNICKERITFVSPIKDGEDI
                     IGVVCFASFNDVQDQIIISKKDEYMTMLRHFAKNIETEISNIKMVNALNIEKAEINAV
                     IDSINKGIIILNSEKKITHINTKAIKTLGINISSIKIINSKISDIIGNIKLEETNEKE
                     LSGRWTVGKRILNVLYKISYILLENNDISTLISFESINEIFNIALKFQSDNIITFENI
                     IRHSYVMEDIISKAKIAARSDSTVFLKGESGTGKELFARSIHNASMRKNNPFIVINCA
                     TLPENLIESELFGYEKGSFTGASPKGKIGKFEQANNGTLFLDEIADLPLHLQSKLLRV
                     LQEKSIEKIGSTDQIDVNVRIISATHRNLEDMVKRNEFREDLYYRLNVIPLHIPALRE
                     RENDVILITEYIINKLCKKFNKTEKHISKDVEEFFLTCPWHGNVRELENVLEYAINFS
                     TEDEIRIEDLPDYFLNSDRDKKFPREDVEFEIQDLMNIKSLEKATREYEKRILKNLLE
                     MYSGNKDTKKIIAKKLNISVTTLYRKLNDYYLND"
     assembly_gap    2059..2158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2159..2818)
                     /locus_tag="PKK75_07275"
     CDS             complement(<2159..2818)
                     /locus_tag="PKK75_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNZ83438.1"
                     /translation="METKKETAIKALKAAFPQTIPILAGYVFLSIAYGVYMNAFGFNF
                     LYPMFMSMLIYAGTVQFVAVGFLLGTFDPVNALALTLMINARYLFYGISMLEKYRFPG
                     IKGFFLIFGMSDETFSINLGANIPEDVDKGWFMLFVTLLNYSYWVSGSILGGLFGELI
                     EFNTKGLEFAMTSMFIVIFLEQWLKEENHKSSLLGLGVSLLCLIIFGSGNFIIPSMLI
                     II"
     gene            3003..3146
                     /locus_tag="PKK75_07280"
     CDS             3003..3146
                     /locus_tag="PKK75_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83439.1"
                     /translation="MLGLDLDFDLIAVVMFFIILISIQFTLNKIYMLLKEIKAILLTR
                     RDK"
     gene            3206..3403
                     /locus_tag="PKK75_07285"
     CDS             3206..3403
                     /locus_tag="PKK75_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HNZ83440.1"
                     /translation="MAEKKNKEKKTRKKEPTPNDIMKLEIAEELGLTDKVQKLGWGGL
                     TAKETGRIGGLMTAKKKKRKE"
     gene            3403..4122
                     /locus_tag="PKK75_07290"
     CDS             3403..4122
                     /locus_tag="PKK75_07290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ83441.1"
                     /translation="MQNSYKEFSQIYDLLMDMDYEKWTSFIMKKLEGKRKVLEAACGT
                     GGVTTLLAENNIKVTAFDLSGDMLMRAYEKLNHSPMVRLLNQNMVDFKIDEKFEAAIC
                     CCDGINYLNREEVSSFFDRIYEHLEEGSKFIFDMSTKYKYDTMFCDTYVYDDGDIFYV
                     WENEAVDEGIINIEINFFIKDHDKYTRIREEQTQYVHEKEEIVRLLEDSGFKNIEVFD
                     DYNEKEYTDSSLRAVFSAVKL"
     gene            4141..5019
                     /gene="hslO"
                     /locus_tag="PKK75_07295"
     CDS             4141..5019
                     /gene="hslO"
                     /locus_tag="PKK75_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNZ83442.1"
                     /translation="MDYMIRAMDKKQTFRLFMVRSTNTVEEARKHHNTTPTASAALGR
                     TLTAGLMMGYMMKNEKDRLTININGGGPIGTILVVSDNSGHVKGYVDNPNIDLDLKEN
                     GKLNVGGAVGTEGKLTVIMDIGMKEPYVGSTRLATGEIGEDIALYYYLSEQQNTAAAL
                     GVLVDIDYSIKAAGGFIVQTLPFIEEEDLVKLENTLNSLKSVSDYFDSQKDVEEIAKV
                     IFSDFEIEIMDQIPVSFSCDCSEERMEQALISIGKEDLKQIIREDEKIETLCHFCSKK
                     YLFEGDKLKNILKYIE"
BASE COUNT         1881 a          739 c          979 g         1385 t
ORIGIN      
        1 gtatcggttt ttttaatgaa aatttagtgt taatttgttt ataatatgtt attatatatt
       61 tattaatttt tattttagct taaatcggag gatcttatgt cgtacttaag gctaatagaa
      121 gataaaattc aaagctttgc agaatcaatg tcatctacgc tgaatgttga gataacggtt
      181 gttgataaca atcttatgag aatagcggga acaggcgatt tttactatcg cataaatatg
      241 accagtccca gtaattcctg gtttgccaga gtgcttaaaa ccggtattga agttatgaac
      301 ttaaacaagg gcagcaattc tgtctgtgcc aagtgtgtcg atttaaacat ttgcaaggag
      361 cgtattacct ttgtatcccc cataaaagac ggggaggaca tcattggagt cgtttgcttt
      421 gcatctttta atgatgttca agatcaaata ataatcagca aaaaagatga gtatatgacc
      481 atgctgaggc actttgcaaa aaacatagaa acagaaatct caaacattaa aatggtgaat
      541 gcacttaaca tagagaaagc ggaaataaat gcagtaatcg attctataaa caagggcata
      601 ataattttaa acagtgaaaa aaagattaca catataaata caaaggcgat aaagacttta
      661 ggtataaata tttcaagcat taagattata aacagtaaaa tttcagatat aattggcaat
      721 ataaagttgg aagaaacaaa tgagaaggaa ttaagcggca gatggacagt ggggaagaga
      781 atactgaatg tattgtacaa aataagctat atattgcttg aaaacaatga catttcaaca
      841 ctcataagct ttgaatcaat aaatgaaata tttaatatag ctttgaaatt tcaaagtgat
      901 aatataataa cctttgaaaa tataatcaga cacagttatg ttatggaaga tattatttca
      961 aaagcaaaga ttgctgccag atctgactca acagtatttc ttaaaggtga aagcggtaca
     1021 ggaaaagaac tttttgcacg gtcaatacac aatgcaagca tgagaaaaaa caatccgttt
     1081 attgtgatta attgtgcaac attaccggaa aacctcatag aatctgaatt attcggttat
     1141 gaaaaaggat ctttcaccgg cgccagccca aagggcaaaa taggaaaatt tgagcaggct
     1201 aacaatggaa ctttgtttct tgacgaaata gctgatctgc ctttgcacct gcagtcaaaa
     1261 cttctaagag tgcttcagga aaaaagcata gaaaagatcg gcagcactga ccagatagat
     1321 gtaaatgtga ggataatttc cgctacccac agaaatcttg aagatatggt aaaaagaaat
     1381 gaattcagag aagatttgta ttacaggctt aatgttattc cattgcatat acctgcattg
     1441 agagaaaggg agaacgatgt aattttaatt acagagtata tcattaataa actgtgcaaa
     1501 aaatttaata aaactgaaaa acatatttcc aaagatgtag aggagttttt tttgacttgc
     1561 ccctggcacg gtaatgtaag agagctggaa aatgtactgg aatatgcaat taattttagt
     1621 actgaagatg aaataagaat tgaagatctg cctgattact tcttaaacag tgacagggat
     1681 aaaaaattcc ctcgagaaga tgtagaattt gagattcagg atttgatgaa tataaaaagc
     1741 ctggagaagg ccaccaggga atatgaaaaa aggatactta agaatctttt ggaaatgtat
     1801 agcggaaaca aagacactaa aaaaataata gcgaaaaagt taaatataag cgttacaacc
     1861 ctttacagaa aactaaatga ttattatcta aatgattaga atctgaaaag cagctgattg
     1921 catacctaag ggagattata aaataatact taaatactaa aagacaatct tacagcgtga
     1981 ggttgttttt tattaaaaaa ccatttgcac caaaaccata taaattacag ttcctgctgc
     2041 aatggaaaga agcatctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
     2161 taattataag catggaaggt attataaagt tgccgcttcc aaagataata aggcataata
     2221 gggatacccc taatcctaaa agtgagctct tatggttttc ttctttgagc cactgttcaa
     2281 ggaaaatcac aatgaacatg gatgtcattg caaattcaag gcctttggta ttaaattcaa
     2341 tcaattcacc gaacaatcct ccaaggattg agccggaaac ccagtagctg taattcaaca
     2401 gggttacaaa gagcatgaac cagcccttat caacatcttc cggaatgttt gcacctaaat
     2461 tgattgagaa agtttcatca ctcataccga aaataagaaa aaagcctttt atgccgggaa
     2521 atctgtattt ttcaagcatg gaaatcccat aaaacaaata gcgggcatta atcataagtg
     2581 ttaaagccaa ggcatttacc gggtcaaagg ttcccaaaag gaaccctact gcaacaaact
     2641 gaacggtgcc agcatagatt aacatgctca taaacatggg atataaaaaa ttgaatccga
     2701 aggcattcat gtataccccg taggcaatgc tcaagaatac atagcctgct aatatcggaa
     2761 tagtctgtgg gaaagctgcc ttaagagcct tgattgcagt ttcttttttt gtttccatca
     2821 tttgaatgtc ccctgacttt aatatttgat atattctaac acttatattt tttattatca
     2881 attagaaatg caaaaaaatg cgaataggat ttctcccctg ctcaagggtg ttttttcttg
     2941 atatgacagt tttcttatga tattattatg aaaaaggcac ggtctcattt caaggagaaa
     3001 atatgttagg tttagattta gattttgatt taatagccgt tgtaatgttc tttataattt
     3061 taataagcat acaatttaca ttaaataaaa tatatatgct tttaaaagaa ataaaggcga
     3121 ttttacttac tagaagggat aaataatccg gtatatagaa taattttctt tttttaaacc
     3181 ataatatttt aaacaggagg gtattatggc cgaaaagaag aataaagaaa agaaaaccag
     3241 gaaaaaagaa ccgacaccaa atgatataat gaaattggaa atagcagaag aacttggact
     3301 tacggataaa gtacaaaaac ttggctgggg aggtcttact gccaaggaaa caggcagaat
     3361 cggagggctt atgactgcca agaaaaagaa aaggaaagaa taatgcagaa ctcatataaa
     3421 gaattttctc aaatctatga tttacttatg gacatggact acgaaaagtg gacatccttt
     3481 atcatgaaaa aattagaggg aaaaagaaaa gttctggaag ctgcctgcgg tacaggtggc
     3541 gtaacaacgc ttctggcaga gaacaacata aaggttactg cctttgacct gtcaggggac
     3601 atgcttatga gagcttatga aaagcttaat cacagcccaa tggtccgctt attgaatcaa
     3661 aatatggttg attttaaaat agatgaaaaa ttcgaagcag caatatgctg ctgtgacgga
     3721 ataaattatc ttaacaggga agaagtcagt tcattcttcg ataggattta tgaacattta
     3781 gaagaaggct caaaattcat atttgacatg agcacaaaat acaaatatga tacgatgttt
     3841 tgtgatacct atgtttatga tgacggagat attttttatg tttgggaaaa tgaagctgtt
     3901 gatgaaggaa taataaatat tgaaataaac ttttttatta aagaccatga caaatacacc
     3961 aggattagag aagaacaaac tcaatatgtg catgaaaaag aagaaatagt acgccttctt
     4021 gaggactctg gatttaagaa tatagaggta tttgacgatt acaatgaaaa agagtataca
     4081 gacagctctt taagagcggt tttttctgca gtgaaacttt aaagatgaaa ggataaaata
     4141 atggattaca tgattagagc aatggacaaa aaacaaacct tcagactttt tatggtaagg
     4201 tcaacaaata cagtggaaga agcaaggaag caccacaaca caacaccaac agcatcggct
     4261 gcactgggga gaaccctcac agcaggactc atgatgggat acatgatgaa aaatgaaaag
     4321 gacagattaa ccataaatat aaacggcggg ggaccaatag gcacaatttt ggttgtttcc
     4381 gataattctg gccatgtaaa ggggtatgtt gacaatccaa atattgattt ggatctgaaa
     4441 gaaaacggca agttaaatgt gggaggagct gtaggaactg aaggcaagct tactgtcatt
     4501 atggatatag gaatgaagga gccctatgtg ggaagcaccc ggctggcaac aggagaaata
     4561 ggggaggaca tagcactcta ttactatttg tcggagcagc aaaatactgc agcagccctg
     4621 ggagttttag ttgacattga ttacagcatt aaagctgcag gaggctttat agttcagaca
     4681 ctgcccttta tcgaggaaga ggacctcgtt aaattggaaa ataccctcaa cagcctaaaa
     4741 tcagtatcag attattttga cagccagaag gatgttgaag aaatagcaaa agtaatattc
     4801 tctgattttg aaattgagat aatggatcaa attcctgtat ccttttcttg tgactgctca
     4861 gaggaaagaa tggagcaggc attgatttca attggcaagg aagatttaaa gcaaataatc
     4921 cgggaagatg aaaaaatcga aactctatgc cacttctgca gcaaaaaata tttgtttgaa
     4981 ggagacaaac tcaaaaatat tctgaagtat atagaatagg aacgtatgca agaacttaag
     5041 cttaaagata gatagcttaa gttctttttt aatttactaa tgga
//
LOCUS       DAOPUT010000096         5062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_16597_length_5062_cov_22.030158, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000096 DAOPUT010000000
VERSION     DAOPUT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16597_length_5062_cov_22.030158"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1075
                     /locus_tag="PKK75_07300"
     CDS             113..1075
                     /locus_tag="PKK75_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNZ83443.1"
                     /translation="MIKAKPLKAGNKVAIIAPSSATDLKAVKQGEMKIKALGLDPIMF
                     PTCYTNYGHLSASDEERAKDVNDAFKDETIKGIICLRGGYGASRILNLLDYEMIAENP
                     KVFLGFSDITALHTAFNQICSMVTFHGPMASTNFARVKNDKVEFDSYSTESLVKNIFT
                     NDPAGLYSNPNNEELKSFCTGNARGQLTGGNLTLLTATLGSKYEIDTKGKILFIEEIG
                     EPVYKLDRMLTTLALAGKFDDCAGIILGTFVKCEREKKAYEGGLDLSLEEVVENTLVK
                     YNKPIIYNFKAGHSFPQPTMALGTKVFIDADVKQVVFIESGTSA"
     gene            1124..2305
                     /locus_tag="PKK75_07305"
     CDS             1124..2305
                     /locus_tag="PKK75_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNZ83444.1"
                     /translation="MDIDRRIDEIYAELVEIRRELHENPELSQNEYRTQGKIRDYLDE
                     HGIENFPCAGTGVVGIIRGKNKGKTVGIRADIDALPIHEKNNVSYSSMNPGVMHACGH
                     DAHTAILLGTGKIIKELADSKDSINGNVKLFFQPAEETIGGAERMVNEGCMEEPKVDY
                     VLGLHVQPYLDAGKVELKYGKLNASTDSISISLKGKAAHGAYPDNGIDAIVMAGSVIT
                     ALQSIVSRNTSPLDSVVISLGKISGGIKDNIIADEVIITGTLRTLDDKTRKYTKERIS
                     SIVDNVAAAFGGEGLTTFHEGYQALINDNEIVDVIRESSEKLFGKENVQFKEFPSLGA
                     EDFSCFLDIAKGAFFHLGCGNSLRGITYPLHTEHFDIDEECLKVGVRLQVENILSLLK
                     K"
     gene            2324..3118
                     /locus_tag="PKK75_07310"
     CDS             2324..3118
                     /locus_tag="PKK75_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNZ83445.1"
                     /translation="MKIFISVDIEGITGVTSWSETEIENQEHKQCAEQMTRETIAACE
                     GAIAMGAKEILIKDAHDSARNINFAQIPKCAKISRGWTNTPDSMVAGLDETFDAAVFI
                     GYHSGSGYDGNPLSHTMSLNNNYIKVNGEIGSEFIINSYLAANYGVPVVFLSGDKMLC
                     ETAKKFNKGIETVAVKEGIGGASISINPELSCELIKEGVKNALKHTTACKIDVPEKFE
                     VEISYKEHTRAKRASYFPGVTQTGPYTVKYTANNINEFSATMMFIL"
     gene            3199..3690
                     /locus_tag="PKK75_07315"
     CDS             3199..3690
                     /locus_tag="PKK75_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNZ83446.1"
                     /translation="MFREMRRIKQLLSMEDTKAVMDRCTNGVLACLGDEDYPYAVPLS
                     YVYFNDKIYFHSGKAGHKIDAVINHPKVSFAVIDKDTIVSEEYTSYFRSVIAFGKARI
                     TEGDEWQEAFKALVEKYSGELPEDDKHKKITECTRSYIIAIDIDHITGKEAIEIVNAK
                     LVK"
     gene            3744..>5062
                     /gene="rlmD"
                     /locus_tag="PKK75_07320"
     CDS             3744..>5062
                     /gene="rlmD"
                     /locus_tag="PKK75_07320"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNZ83447.1"
                     /translation="MLEKGLVKEVEIIDMNHIGQGVARIDNFVVFVSDAITFDMVKIK
                     ITDTKKNYATGEIIEIIKPSRYRIKPDCAYSKQCGGCQLTHVDYKEQLKYKRNRVVSE
                     FRRAGVSFDEETIYDTVGMDEPYRYRNKTAFSVTEKNNEIQIGPYEQGTYNTVDINSC
                     LLQSKAADDLVKAFKNLMEKYNIKAYDKKTKKGIVRNIVIRNNRKNEIMLIIVTAAEN
                     FQNKALLIDELSQIQNIKTIVQNINNKNTNLIMGSKSIILYGEGVIIDTIGDLSFTIS
                     SETFFQINPLQTEKLYQTAIEYADIHKNDICFDIYCGIGTISLMAAKKAKKVYGVEIV
                     DQSIINARENAEINNISNVEFYTGKAEEIIPELYKQNIKADIAILDPPRKGCEKEVID
                     TIINMAPKKVVYVSCNPSTLARDIKLLEQGGYKLIKARPVDQFPWSV"
BASE COUNT         1871 a          816 c         1105 g         1270 t
ORIGIN      
        1 gggcaggaaa taacaaaaat agtatacgaa ttatatgacg tgcctttgtc tgcaagttca
       61 aatggagaaa atattgccca tgcttcggag taacgaaaaa gggggaaaaa caatgattaa
      121 agcaaaacct ttgaaagcag ggaacaaggt tgcaataata gccccgtcat cagcaactga
      181 tttgaaagct gtgaaacagg gtgaaatgaa aataaaagca ttagggttag accccataat
      241 gtttccaacc tgttatacaa attacggaca cctttctgca tccgatgaag aaagggcaaa
      301 agatgtaaac gatgccttta aggatgaaac cattaaaggc ataatttgtt tgcggggagg
      361 gtatggagct agcaggatac taaatctctt ggattatgaa atgatagctg aaaatcccaa
      421 ggtattttta ggattcagcg atattacagc acttcatacc gcttttaatc aaatatgcag
      481 catggtgacc ttccacggtc ctatggcatc aacaaacttt gcaagggtaa aaaatgacaa
      541 ggttgaattt gattcttatt ccactgaaag tctggttaaa aatattttta caaatgaccc
      601 ggcaggttta tacagcaatc caaacaatga agagctaaaa agtttctgca ccggtaatgc
      661 ccggggacaa ttgacaggag gaaaccttac ccttctgact gcaaccctgg gttcaaaata
      721 tgaaatagac actaagggca aaatcctttt cattgaagaa ataggagagc ctgtctacaa
      781 actggacagg atgcttacaa cattggcact ggcaggaaaa tttgatgatt gtgcaggaat
      841 aatactggga accttcgtta aatgcgaaag ggaaaagaaa gcctacgaag gcgggctgga
      901 cctttctttg gaagaagtag tggaaaatac attagtcaaa tacaataaac cgataattta
      961 taatttcaag gcaggacaca gttttccaca gcctacgatg gccttgggta cgaaggtctt
     1021 catagatgca gatgtgaaac aggtagtatt tattgagagc ggaacatcag cttagctatt
     1081 aataaaaaga gatgcataag aggagtttgt cagagaggct catatggata tagatagaag
     1141 aatagatgaa atatatgctg aattggtgga aataagaaga gaacttcacg aaaatccgga
     1201 actcagccaa aatgagtaca gaacacaggg aaaaatccgt gattatttgg atgagcatgg
     1261 aatagaaaac ttcccttgtg caggtacagg cgttgttgga ataataaggg gaaaaaacaa
     1321 aggaaaaacc gtgggtatca gagcggatat agatgctctg ccaattcatg aaaagaacaa
     1381 tgtttcgtac agttcaatga atcccggggt tatgcatgca tgcggccacg atgcccatac
     1441 agcaatactg ctgggtacag gcaaaataat aaaggaattg gccgattcca aagactccat
     1501 aaacggaaat gtcaagctgt tttttcaacc ggcagaagaa acaataggcg gtgcagaaag
     1561 aatggttaat gaaggatgca tggaagagcc aaaggttgat tatgttttgg gacttcacgt
     1621 tcagccttat ttagacgccg gaaaggttga gcttaaatac ggcaagctca atgcgtccac
     1681 agattccatc agtataagtc tgaaagggaa agctgcacac ggtgcatatc ctgataacgg
     1741 aattgatgcc attgttatgg caggcagtgt tataactgca ctgcagtcaa tagtaagcag
     1801 aaacacatct ccattggaca gcgtggtaat atcgttaggc aagatttccg gcggcatcaa
     1861 agataacatt attgccgatg aagtaataat tacaggcact ttgagaacct tggacgataa
     1921 gacaagaaag tatacaaagg aaagaatttc gagcattgtt gataatgttg ccgcagcttt
     1981 cggaggagaa ggacttacga catttcacga aggctaccag gctttgatta atgataatga
     2041 gattgttgat gttataagag agagttctga aaagcttttt ggaaaggaaa atgttcaatt
     2101 caaagagttt cccagtcttg gagcagagga tttttcatgc tttttagata ttgcaaaagg
     2161 tgcatttttt cacttgggat gcggtaattc attaaggggc ataacttatc cccttcatac
     2221 agagcacttt gatatcgatg aggaatgcct taaggtagga gtgaggcttc aggtggaaaa
     2281 tatattatct ttactaaaaa aataaaatat caggaggata agaatgaaaa tatttataag
     2341 cgtggatata gaaggaatca ccggagttac gtcatggagt gaaacggaga ttgaaaatca
     2401 ggaacacaaa caatgcgcag agcagatgac cagggagaca atagctgctt gcgaaggtgc
     2461 aatagccatg ggagcaaaag aaatattaat aaaagatgct catgattctg caaggaacat
     2521 aaattttgca caaattccca aatgcgcaaa aataagcagg ggctggacaa atacaccgga
     2581 ctccatggtt gccggactgg atgaaacatt cgatgcagct gtttttattg gatatcattc
     2641 aggatcaggt tatgacggaa atccgctgtc tcatacaatg agcttaaata acaattatat
     2701 aaaggtaaat ggtgaaatag gatcagaatt tataataaat tcttatttgg cagcaaatta
     2761 cggtgtgcct gtggtatttt taagcggaga taaaatgctt tgcgaaactg ccaagaaatt
     2821 caacaaaggc atagaaaccg ttgctgttaa ggaaggaatt ggaggggcat caatcagcat
     2881 caatcctgaa ttgtcctgcg aattgattaa agaaggagtt aaaaatgccc ttaagcatac
     2941 tacagcatgc aaaatagatg tccctgaaaa atttgaagtt gaaatcagct acaaagaaca
     3001 caccagggca aaaagagcat catattttcc tggagttact caaacagggc cttatacagt
     3061 taaatataca gcgaataata ttaatgaatt ttctgcaaca atgatgttca ttttataaaa
     3121 tcaaaggaga caacgagtgg ggcactgtgg gtgtaagcta actgtcccgc atcattttat
     3181 tgaaagagag gatatattat gtttagagaa atgagaagaa taaaacaatt attatcaatg
     3241 gaagatacta aagccgtgat ggacaggtgc acaaacggtg tactggcatg cctgggggat
     3301 gaagactatc cttatgcagt gcccttaagc tatgtttatt ttaatgataa aatatatttc
     3361 cattcaggaa aggcaggcca taaaatagat gctgtcataa accatcctaa ggtgtctttc
     3421 gcagtaatag ataaggatac tatagtaagc gaagaataca cctcatattt tagaagtgta
     3481 atagcttttg gcaaggcgag aattacggaa ggcgatgaat ggcaggaagc atttaaggca
     3541 ctggttgaaa agtattccgg agaattgcct gaagatgata agcataagaa aattactgaa
     3601 tgcacaaggt catatattat cgcaatagat atcgaccaca taacaggcaa ggaagctatt
     3661 gaaatagtca atgcaaagct ggttaagtaa gtatttcatg ttttgtctta atagcttaat
     3721 aatgtaaaac accgggagaa gtcatgcttg aaaaaggact ggtaaaagaa gtagaaatta
     3781 tcgatatgaa tcatataggg cagggagttg ccagaattga taattttgtt gtttttgtaa
     3841 gtgatgcaat aactttcgac atggttaaaa taaaaataac agatacaaag aaaaattatg
     3901 caacaggtga aataatagaa attattaagc cctctcgata tagaatcaag ccagactgtg
     3961 cttattcgaa gcagtgcgga ggctgccagc tcacgcatgt ggattacaag gagcagttaa
     4021 aatacaaaag aaacagagta gtcagtgaat tcaggagagc cggggtaagt ttcgatgaag
     4081 agactattta tgataccgta ggcatggatg agccatacag atacagaaat aaaacagcat
     4141 tttccgtaac agaaaaaaac aatgaaatcc aaatcggacc atatgaacaa ggcacataca
     4201 atacagttga cataaacagc tgcctgctcc aaagcaaagc cgctgatgat cttgttaaag
     4261 cctttaaaaa tttgatggag aaatacaata ttaaagccta tgataaaaaa acaaaaaaag
     4321 gcattgtaag aaatattgtc atcagaaata atagaaaaaa tgaaattatg cttatcatag
     4381 tcactgctgc tgagaatttt caaaacaaag ccctattgat agatgaatta tctcaaatcc
     4441 agaatattaa aacaatagta caaaacataa acaataagaa cactaattta ataatgggca
     4501 gcaaaagtat catcttgtat ggggaaggtg tcataattga tacaatcggc gacttaagct
     4561 tcaccatatc ttcggaaaca ttctttcaaa taaatccgct tcaaacagaa aagctctacc
     4621 aaactgcaat tgaatatgca gatattcata aaaacgacat ttgttttgat atttactgcg
     4681 gcatagggac catatcctta atggcagcaa agaaggcaaa aaaagtttat ggcgttgaaa
     4741 ttgttgacca atcaattatt aatgcaaggg aaaatgccga aataaacaac atatcaaatg
     4801 ttgaatttta taccggtaaa gcagaagaaa tcattcctga actctataag caaaacatca
     4861 aggcagatat agcaatccta gaccctccaa gaaaaggctg tgaaaaagag gtaatagata
     4921 ccataataaa tatggcacca aagaaagttg tttatgtatc atgcaatccg tcaaccttgg
     4981 caagagatat caaattattg gagcaaggag gctataaatt aataaaagcc cggcccgtag
     5041 accaattccc atggagcgtg ca
//
LOCUS       DAOPUT010000097         5050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_16646_length_5050_cov_19.556156, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000097 DAOPUT010000000
VERSION     DAOPUT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16646_length_5050_cov_19.556156"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1060
                     /locus_tag="PKK75_07325"
     CDS             143..1060
                     /locus_tag="PKK75_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ83448.1"
                     /translation="MAINKVVRAALKALSYNELDIKKNLQMHRNFVNMTRRQILKPLF
                     KTRDYKIGSKNYNIPVRIFSNHVNSDFPVLLFFHGGGWVTGNIDSYNKVCINIARLTD
                     CKVVSVDYRLAPENPFPAGLEDCYNAAKAFITNDVINDKEVILIGDSAGGNLAAALSL
                     LAKERKEFAVNKQILIYPSTYNDHTDKSPFKSVMENGTDYLLTSKRIRDYMDLYKSRE
                     EDVYSPYFAPLLSNDLSMQPRTLIITAEYDPLRDEGEEYGRKLREAGNYVEIYRIKDA
                     LHGFFALPPRFPQVKLCYDIINRFIYEVD"
     gene            1061..2344
                     /locus_tag="PKK75_07330"
     CDS             1061..2344
                     /locus_tag="PKK75_07330"
                     /inference="COORDINATES: protein motif:HMM:NF018902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83449.1"
                     /translation="MKNKKISEWSKLDNAAKIFPANSKKSDTKVFRFACELFDTVDPQ
                     VLQEALDITLEAFPLYQSVIKSGLFWYYFEKSHFKALVTEENIPPCSTLYDKNNKTLL
                     FRVMYYRKRISFEVYHALTDGAGALQFLRTLVFHYITIKYEHALKDKSPVLDIEASKT
                     QKLDDSFQKHYKDEKGSSKKKKIKAFKIKGSKIPEYRIRVIEGIIPVDAILPKVKENK
                     ASLTIFLAAVLMCAINEEIPARLKKRPVVLSIPVNLRNYYSSQTARNFFGVINVDYDF
                     SKGNDDLTEVIEALKKKFKENLTPEVLARRINKLASLEHNYVLRLIPLKIKDFALKIA
                     YALADRSYTSTLSNVGIVSMPDDIKPFIHSFDVFVSTDKIQACLCSFENQLRISFTSA
                     FTSTEIQRRFFKTLTDMGIPLVIDSNIIDEEQEAL"
     gene            2341..3006
                     /locus_tag="PKK75_07335"
     CDS             2341..3006
                     /locus_tag="PKK75_07335"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HNZ83450.1"
                     /translation="MMKFCKSCNVSVTGKRKICPLCQGRLTGDKAQEEIFPKISFVYT
                     EHSLFFKVMLLTSIIAATVSVAVNILMPGGGAWSLFILGGLASVWASLITIINQRKNI
                     PKNIVYQVMTISVTALIWDTLTGWRGWSINYIIPLVCVFAMISMAVISKIRKLHIEDY
                     ILYIIIDSLFGIVPVIFIILGLLDVLYPSIICISTSIISLSTIIIFEDKSLKAEIKRR
                     LHV"
     gene            complement(3114..4265)
                     /locus_tag="PKK75_07340"
     CDS             complement(3114..4265)
                     /locus_tag="PKK75_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ83451.1"
                     /translation="MKEKKNLFLILTLFYIYFIIALFESSRGNFVPFFLEEFRINNTQ
                     MSLVLTFNSIGSIIGSYLGGHLCERYGHKYVFFIGALVSTTAVLIAPFASSLFLLGLF
                     NFLFGIGRQLWAVSVDSAIPVLSIGFESILMNLTHFMYGLGSLTGQTMYGRLLEYGLH
                     WRSIYLYLGVFFLLSAVLTLLVRVPRIKVSAGREVKRRELYKNPVIYLFVAAVTFEFL
                     SESVIYTWFVSYTRSSYGFSPAAAAKYASVYYLLFASGRLAGGFILNKTGNLRGLQIY
                     LLTGALLIFSGLLLRENGLLIIAASGFFISVVFPTLMVIAYKVFKESTSYAIGYITTL
                     ANIFFMILFNVTGALNDFAGTYGAFFIAPASMIACFAIISIINKRAVNE"
     gene            4462..5037
                     /locus_tag="PKK75_07345"
     CDS             4462..5037
                     /locus_tag="PKK75_07345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ83452.1"
                     /translation="MFRQSIYQTDRLYLKILKAGYYRQVLEYYRKNHNFLKEWEPKRP
                     KDFYTPAYQKSQLSYEYNMFKSRKLIRFWIFKKEDNKLIGNICFSNIIMGNFKSCFMG
                     YKLDNDEINKGYMTEAIREAVRIMFDDYGLHRIEVNILPRNIRSLTVMKKLNFEQEGY
                     SKRYLEINGKWEDHIHFAIYSEYEETPSSIG"
BASE COUNT         1741 a          907 c          982 g         1420 t
ORIGIN      
        1 aaaataatac atgctaattc tgattatttg atcgaataat aaagctgtta aacaagaaaa
       61 gggcaccctt ccataattat tttgatagac acatataata tttatatata taatagtgta
      121 aacaaacaaa taagagggaa atatggcaat aaacaaggtt gtaagggctg ccttaaaagc
      181 tttgtcctac aatgaacttg atattaaaaa aaacttgcag atgcacagaa attttgtaaa
      241 catgaccaga aggcaaattt taaaaccctt gttcaaaaca cgggactata aaatcggttc
      301 aaaaaactac aacattcctg tcaggatatt ttcgaatcat gtaaattcgg actttcctgt
      361 cctcttgttt ttccatgggg gcggatgggt aacaggcaat attgactcct ataacaaggt
      421 ttgcataaat atagcaagac ttacggactg caaggttgtt tccgtggact acaggctggc
      481 accggaaaat ccctttcccg ccggtttgga ggattgttat aatgcagcca aggcatttat
      541 tacaaatgat gtaatcaatg ataaggaagt aatactgata ggggacagtg caggaggaaa
      601 cctggcggca gccttatcct tgctggcaaa ggaaagaaaa gaatttgccg ttaacaaaca
      661 aatattgatt tatccttcaa cctacaatga ccacacggac aagtcccctt ttaagtctgt
      721 tatggaaaac gggacggatt atctgcttac ttcaaagaga ataagagact acatggactt
      781 gtacaagtcc agggaagaag atgtatacag cccttatttt gcacctttgc tttcaaatga
      841 cctgtccatg cagccaagga ccttgattat aactgcagaa tatgacccct tgcgcgacga
      901 aggtgaagaa tacggcagaa aactgaggga ggcaggtaat tatgttgaaa tatacaggat
      961 taaagacgcc cttcacggtt tttttgcact tcctccaagg tttcctcagg ttaaattatg
     1021 ttatgatata attaatcggt ttatttatga ggtggattaa ttgaaaaaca aaaagatatc
     1081 tgaatggagc aaattagata atgcggctaa aatatttcct gccaacagca aaaaaagcga
     1141 taccaaagta ttcagattcg catgtgaact gtttgatact gtagaccccc aggtgcttca
     1201 ggaggcactt gatattactt tggaagcatt tcccctgtat cagtcagtga taaagagcgg
     1261 tcttttctgg tattattttg aaaaaagcca ctttaaggct cttgtcacag aagaaaacat
     1321 tcctccatgc tccaccttgt acgataaaaa caataaaact ctgcttttca gggttatgta
     1381 ttacaggaag agaataagct ttgaagtata tcatgcattg actgacgggg caggagccct
     1441 gcagtttttg agaacattag ttttccacta catcaccata aaatatgaac acgctttaaa
     1501 agacaaatct cctgtcttgg acatagaggc ttcaaagacg caaaagttag atgacagttt
     1561 ccaaaaacac tataaagacg agaaaggctc ttcaaagaaa aagaagataa aggcatttaa
     1621 aataaaaggc tcaaagatac ccgaataccg catcagagta atcgaaggta ttataccggt
     1681 tgatgcaata ctgcctaagg tcaaggaaaa caaagcatca ttgacaatat tcttagcagc
     1741 ggttttaatg tgtgcaataa atgaggaaat ccctgcaaga cttaaaaaaa ggccggttgt
     1801 actcagcata cccgtgaatt taaggaacta ttattcctcc cagactgccc gcaatttttt
     1861 tggagttata aatgtggatt atgatttttc aaagggaaat gatgatttaa cagaagttat
     1921 agaagctcta aagaaaaagt tcaaggaaaa tttaacaccg gaagttttgg caagaagaat
     1981 aaacaagctg gcatccctgg agcacaacta tgttttgagg ctcattccac ttaaaattaa
     2041 ggattttgct ctgaaaattg catatgctct tgcagacaga agctatactt caacattgtc
     2101 caatgtgggt attgtaagca tgcctgatga tatcaagcct tttatacact cattcgatgt
     2161 atttgtgagt accgacaaaa ttcaggcttg tctttgctca tttgaaaatc agctgagaat
     2221 aagctttaca tctgcattta cgagtacaga aatacaaaga aggtttttca aaaccctcac
     2281 tgatatgggg atacccctag taatcgattc aaatattatt gatgaagaac aggaggcctt
     2341 atgatgaaat tctgtaaaag ctgcaatgtc agcgtaaccg gaaaaagaaa gatttgtccc
     2401 ctttgtcagg gcaggctgac gggagataag gcccaggagg aaatttttcc taaaatctca
     2461 tttgtttata cagaacactc cttgtttttc aaggttatgc ttttgacatc aataatagct
     2521 gccactgttt ctgttgctgt taatatactg atgcctggag gcggtgcatg gtcattgttt
     2581 atactgggag ggctggcttc agtttgggca agtctcataa caattataaa tcagcgaaaa
     2641 aacataccca aaaacatagt ataccaggtt atgacaatat ccgtaacagc tttaatctgg
     2701 gatactttaa caggctggag gggctggtcc attaattaca taatacccct ggtgtgtgtg
     2761 tttgccatga tatccatggc ggttatttct aaaatcagaa agcttcacat tgaagattat
     2821 atcttatata taataattga ttctttgttt ggaatagttc ctgtgatatt cataatactg
     2881 ggccttttgg atgttttgta cccgtcaatt atctgtatat caacaagcat tatttcgctt
     2941 tcaacaatta taatttttga agataaaagc ttgaaggcag aaataaaaag aaggctgcat
     3001 gtgtaaagac gcccttaacc ctaatatagc ctttaagagt cgaaagaatg tcccaaacag
     3061 ggtattttag tgatggagac tgcgggacgg tctaaaattt atctacgttg cagttattca
     3121 ttaaccgctc ttttattaat tattgaaatt attgcaaagc atgcaatcat tgaggcagga
     3181 gcaataaaaa acgctccata ggtccctgca aaatcattta aggcacctgt aacattaaag
     3241 agtatcataa agaaaatatt agccaatgtt gttatatagc cgattgcata tgaagtgctt
     3301 tctttaaaaa ctttatatgc aattaccata agtgtgggga atactacgga tataaaaaat
     3361 cctgaagctg ctataatcaa aagtccattt tccctcagaa gcaagccgga aaaaatcaaa
     3421 agagccccgg tcagcaagta gatttgcaga ccccttagat tgcctgtttt gttcaggatg
     3481 aatcctcccg ccagtcttcc ggaggcaaac aacaggtaat aaactgaagc gtattttgct
     3541 gctgcagcgg gactaaatcc gtaggagctt ctcgtgtagc ttacaaacca tgtatatatg
     3601 acggattcgc ttaaaaattc aaatgtaaca gctgccacga acagataaat tacaggattc
     3661 ttatataatt cccttctttt tacctctctg ccggcgctga cctttatccg gggcaccctt
     3721 accagcaagg tgaggactgc ggataataga aagaaaacac ccagataaag ataaatactt
     3781 ctccaatgga gaccatattc aagcagcctg ccgtacatgg tctgacctgt caagcttccc
     3841 aagccataca tgaaatgggt tagattcatt aaaattgact caaaacctat ggacaaaaca
     3901 ggtatggccg agtcaacgga aactgcccat aactgcctcc ctattccaaa taaaaaatta
     3961 aaaagtccca gcaaaaaaag actggatgca aaaggtgcaa tcagtacagc agtggttgaa
     4021 acaagggctc ctatgaaaaa aacatatttg tgtccgtacc tttcacaaag atgtcctccc
     4081 aaatagcttc ctattatgct gcctattgaa ttaaaagtca gcaccagact catctgtgtg
     4141 ttgttaatcc ggaattcttc cagaaagaaa ggaacaaaat ttcctcttga actttcaaaa
     4201 agagctatta taaaatatat gtaaaataag gtgagtatta aaaataaatt ctttttctct
     4261 ttcaattgct ctcctgaatc ttgataataa tatgcgggga caatcttcaa aaggtcttgc
     4321 cgaataaaca tctaatggaa agtgtccccc tttcactaat gataaccata ttatcataaa
     4381 aataatttgt ggtaaagcga attcagttga ctaaatacat gaaggattat aaaattaaat
     4441 gatacttgcg ggagggctgc tatgtttaga caatcaatat accaaacaga cagattatat
     4501 ttaaaaatat taaaggccgg ttattacagg caggttttag aatactatcg taagaaccat
     4561 aattttttaa aggaatggga accaaaaaga cccaaggact tctatacacc ggcatatcaa
     4621 aaaagtcagc tcagttatga atataatatg tttaaaagca gaaagctcat aaggttttgg
     4681 atttttaaaa aggaagacaa caaattaata ggcaatatct gctttagcaa tataataatg
     4741 ggaaacttta agtcctgctt catgggatac aagctggaca atgatgaaat aaacaagggg
     4801 tacatgacag aagcaataag agaagcggtt cggattatgt ttgatgacta cggtcttcac
     4861 agaattgaag taaatatact gccgcggaac attagatcct tgacggtgat gaaaaaactt
     4921 aattttgagc aggaaggcta ttcaaagagg tatttggaga taaacggcaa atgggaagac
     4981 catattcact ttgcaatata ttcggagtac gaggaaacac cttcttctat tggataatcc
     5041 ttgaggggtg
//
LOCUS       DAOPUT010000098         5044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_16677_length_5044_cov_17.562437, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000098 DAOPUT010000000
VERSION     DAOPUT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16677_length_5044_cov_17.562437"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PKK75_07350"
     CDS             <1..1055
                     /locus_tag="PKK75_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ83453.1"
                     /translation="KTLGGNMKKISFLLAVVLVFGLLAGCSNEPEEKTPEPGEPIETP
                     VEENPGVEAVDPVNVTIAGLKGPTSIGMIKMIDEKALQSDKYNVEYIQEAAPDSLTGK
                     IINGDIQISSVPINLASVLYNKTQGKVQLAAVNTIGNLFIVGTDNISSISQLEGKILG
                     MSAKGSTPDFAMNYILKQAGLEGKTELNYALDHASLAQSVIAEDTAIALLPQPFVTQT
                     RLKNPNVKMLIDLNEAWNDATGGKSELYTGCIIINKEFAENNPEFVSEFLEQYENSVN
                     WVLENQKDASVLVAKHEIMPDAVLVEKALPYCGITFKKAMDAKEGLNDFYQILFDSNP
                     ASVGGSMPDDEFYFTE"
     gene            1030..1779
                     /locus_tag="PKK75_07355"
     CDS             1030..1779
                     /locus_tag="PKK75_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ83454.1"
                     /translation="MMNSILRNKNKLLIILFWILVWEILSLIIAQEIYLPSPHAAFSA
                     LLNLLTSKDTYITIFFSTYRTLGGFFLSCVFGIFLGIMSGIYKPMYDLLNPLIIIMRT
                     IPVMSIIIIAIMWFKDTNVPIFVAFLMCFPIIWTNTVSGIRSTDVKLLQMCEVYKIKR
                     IRVIKSVYFNSSLPYIQAGMVSALGIGWKVTSAAEVLSLPKYSIGRFLYDSKVYLEIP
                     GLFAWTIIIVSLSFLFEALLKIFFKFGDKYD"
     gene            1772..>2050
                     /locus_tag="PKK75_07360"
     CDS             1772..>2050
                     /locus_tag="PKK75_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ83455.1"
                     /translation="MIKLIDVSKKFDDLTLLKDFNISFEKNKITCLFGPSGAGKTTIA
                     NIVAGIVPVDKGCVEGVDSSLFSYVFQEPRLLKWYSAYENIDFVLKDVY"
     assembly_gap    2051..2150
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2624..2773
                     /locus_tag="PKK75_07365"
     CDS             2624..2773
                     /locus_tag="PKK75_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83456.1"
                     /translation="MKQSENDKLYKVDGFDIIVTGDSQFELKGAKIDYAGLLFKDFIV
                     SPKFS"
     gene            2786..3064
                     /locus_tag="PKK75_07370"
     CDS             2786..3064
                     /locus_tag="PKK75_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83457.1"
                     /translation="MYKLWAKKIKNNRIINSVTVSNKDNISNEEKRKKCLDEICKRLD
                     VSIPVWLKKHDMEFTQFKYVTLYAEDFIDEIDFDKLEIELLDNEKNKK"
     gene            complement(3103..3381)
                     /locus_tag="PKK75_07375"
     CDS             complement(3103..3381)
                     /locus_tag="PKK75_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83458.1"
                     /translation="MECNKPANIEEVKDEVSAEANAQCDCVRGDRGRRGDSVRRDRVL
                     DTGGADSSLLFFFLLLVVIVCQSDCDIELETLLWFFLLLVALFNFCDC"
     gene            3549..>3742
                     /locus_tag="PKK75_07380"
     CDS             3549..>3742
                     /locus_tag="PKK75_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HNZ83459.1"
                     /translation="MDKFALILVIIGAINWGLIALFQFDLVAAIFGGQTVLFSRLVYG
                     LVGLAGLYCITLLFTERERV"
     assembly_gap    3743..3842
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3881..>5044)
                     /locus_tag="PKK75_07385"
     CDS             complement(3881..>5044)
                     /locus_tag="PKK75_07385"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNZ83460.1"
                     /translation="YSYTEKVDETSIDQLVKDAAENALIIDSEDEEYIFEGSKEYKKV
                     DSFNQALSNVGEEQKINFVKSLEEEAFRLDKRIASVEVCIYGDGYGETIMSNTKGLFL
                     RDKSNIAYTYIAVVAKDGEDIKTGMAYRTGNDFNAFNAQDIAREAVDEALALLGARSV
                     KSGDYNVIIRNSAAADLMEAFTGIFSAEAVQKNLSLLKGKLNEKIASDKFTLIDDPYM
                     EGGLASKSFDGEGVACKYKKVVDKGVLKTYLYNLKTAKKDGVDTTGNASKGSYKSSIG
                     ISPSNFFVEKGERTLDEMIADIDKGILITELQGLHSGLNSVSGDFSLAALGFLIQDGK
                     ISRPVEQITMAGNYFDMLKNIEETGSDLKFGMPGEAYIGSPSLRIKKLSIAGE"
BASE COUNT         1523 a          850 c          896 g         1575 t
ORIGIN      
        1 aaaaaacatt aggaggcaat atgaaaaaaa tcagtttttt attggcagtt gttttagtat
       61 ttggattgct ggcaggctgc agcaatgagc ctgaagaaaa gacacctgag cctggtgaac
      121 ccattgaaac accggttgaa gaaaatcccg gagtggaagc tgttgaccct gtaaatgtta
      181 caattgcagg acttaaagga ccaacatcca taggaatgat taagatgatt gatgaaaagg
      241 cacttcaaag cgataaatac aatgttgaat atattcagga agcagcacct gattcattga
      301 caggaaaaat tataaacggg gatatacaga tatcgtctgt tcctataaat ttagcctctg
      361 tattatacaa taaaactcaa ggtaaggttc aattggcagc tgttaacaca atcggaaact
      421 tatttatagt cggaactgat aatataagct caatttccca gctggagggt aaaatccttg
      481 gaatgagtgc aaagggttca actcctgatt ttgctatgaa ttatatatta aaacaagcag
      541 gtcttgaagg caaaaccgaa ctaaactatg ctcttgacca tgcaagtctg gcccagtctg
      601 taatagcaga agatacggca attgccctgt tgccccagcc ctttgtaact caaacaaggc
      661 tgaaaaatcc caatgttaag atgcttatcg atttaaatga agcatggaat gatgcaacgg
      721 gcggaaaaag cgaattgtat accggatgta ttatcataaa caaagagttt gcagaaaaca
      781 atccggaatt tgtcagtgaa tttttagagc aatatgaaaa ctcagttaac tgggtattgg
      841 agaatcaaaa ggatgcctct gtattggttg caaagcatga aattatgcct gatgcagtct
      901 tggttgaaaa agctctgcct tactgcggaa taacttttaa aaaagcaatg gatgcaaaag
      961 agggcttgaa tgatttttac caaatattat ttgattcaaa ccctgcttca gtaggaggaa
     1021 gcatgccgga tgatgaattc tattttacgg aataaaaata aattattgat tatacttttt
     1081 tggattcttg tttgggagat actgtcgctg ataatcgctc aggagattta tctcccttct
     1141 cctcatgctg cattttcagc tcttttgaat ctgctgactt caaaggatac ctatatcact
     1201 atttttttca gcacttacag aacattgggg ggattttttt tatcctgtgt ctttggtata
     1261 tttttaggaa ttatgagcgg catttacaag cccatgtacg atttgcttaa cccattgata
     1321 attattatga gaacaattcc cgtaatgagt ataataatta ttgcaattat gtggttcaag
     1381 gataccaatg taccgatttt tgttgcattt ttaatgtgtt ttcccataat ctggaccaat
     1441 acggtttcag gaatccggtc aactgatgtt aagcttcttc aaatgtgcga agtttataaa
     1501 ataaaaagga taagagtaat aaaatctgta tattttaatt cctctcttcc atatatacag
     1561 gcaggcatgg tttctgctct tggtataggg tggaaggtta cctctgcagc ggaagttctc
     1621 agtctgccca agtactccat cggaagattc ttgtacgatt caaaggtata tcttgaaatt
     1681 cccggcttgt ttgcatggac aatcataatt gtttcactga gctttctgtt tgaagctctg
     1741 cttaaaattt ttttcaaatt tggagataag tatgattaaa ttgattgatg tttcaaaaaa
     1801 atttgatgac cttacactat tgaaggattt taacatcagt tttgaaaaaa ataaaattac
     1861 atgtttgttc ggtccttcag gtgcggggaa aacgaccatt gcaaatattg tggcaggtat
     1921 tgttcctgtt gacaaaggct gtgtggaggg tgtggactct tcattgtttt cctatgtttt
     1981 tcaggagccc cgccttctta aatggtacag tgcatacgaa aatattgatt ttgtgctcaa
     2041 ggatgtttat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn actaaatcac
     2161 tgattatatt tctttgtggt atatagttta ataaaaacgg aggaatatat atggtcttta
     2221 cattaagtga aaaagcaaaa acaaaattaa atgaaatgaa aaatgaaagt aaacctatta
     2281 aattaaagat aaccggctat tcctgatgcg gtgccaaact cggcattgtt tcggagaaac
     2341 aaaaagatat tgaaaaagta tataatactg aaggtattga tataattgtt tcagaagatt
     2401 tggaaggtgc cataatgggt gctgaaattg attatttaac ttcatttttt atgaaaggat
     2461 ttaatgttac tccaactttt tcaagaggat aggagaattc atgaatattg aaataaccgc
     2521 catggcaaaa gaaaagctgc ttttgatgaa ggaaaatgat atgcccatag ctataatgcg
     2581 atatccggta ggttgagcag gctttaaata taaaattgtt tcggtgaaac aatctgaaaa
     2641 tgataaattg tataaggttg acgggtttga tataatagtt accggagaca gccagtttga
     2701 acttaaaggt gcaaaaattg attatgccgg cctgttattc aaggacttta tagtgtctcc
     2761 aaagttctca tgagcggaga gctgaatgta taagctttgg gcaaaaaaaa ttaaaaacaa
     2821 cagaattatt aactctgtta ccgtaagtaa caaggataat atttcgaatg aagagaaaag
     2881 aaagaaatgc cttgatgaaa tatgcaaaag acttgatgta agtatccctg tatggttgaa
     2941 aaaacacgac atggaattta ctcaatttaa atatgtcacc ttatatgctg aagattttat
     3001 tgatgaaatt gattttgaca aacttgaaat cgagttatta gataatgaaa aaaataaaaa
     3061 atgaaacctg cttaaactat caagcaggtt tttttgtatg tttcaacaat cgcaaaagtt
     3121 aaataatgcc accagtaaaa ggaagaacca caataaagtt tccaattcaa tgtcacaatc
     3181 gctttgacac actataacaa ccaataacaa aaagaaaaac aacaatgaag aatctgctcc
     3241 tcctgtatct aaaaccctgt ctcttctgac tgaatcccct cgtcttcccc tgtcaccgcg
     3301 tacacagtca cactgtgcgt ttgcttcggc gcttacttcg tcttttactt cttcaatatt
     3361 tgcaggttta ttgcactcca tatataacct cctaattaca atataacgat ttaatttatt
     3421 atattcattt aaaaaatatt tgttaatatt tatcggattt ctttcctttt tagaaattgt
     3481 gtataatctt gaaaaaattt gaaataataa ctctatattc tatttaatat gaggaggata
     3541 gtaagtcatt ggataaattt gcattgatat tagtaattat cggggctata aactgggggc
     3601 tgattgcatt atttcagttt gacttggtag cagctatttt tggcgggcag acagtcttat
     3661 tcagcaggct ggtatacggg ctggttggac tggcaggttt gtactgcatc acactattat
     3721 ttactgaacg cgaaagagta gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnaaaagaca atccatggcg gattgtcttt tttattatcc ttattcccct gcaattgaaa
     3901 gtttcttgat tcttaaagat ggtgagccta tataagcttc tcccggcatg ccgaatttta
     3961 aatcagagcc tgtttcttca atatttttaa gcatgtcaaa atagttacct gccattgtaa
     4021 tttgttccac aggtcttgag attttcccgt cttggattaa aaatcccaag gctgccaatg
     4081 aaaaatcccc tgatactgaa ttcaatcctg aatgaagccc ttgaagctca gtaatgagta
     4141 tccccttgtc tatgtccgca atcatttcat ccaatgttct ctctcctttt tccacaaaga
     4201 aatttgaagg agatatgcct attgatgatt tgtaagaccc tttgctggca tttccggtag
     4261 tatcaacccc gtcctttttg gcagttttca aattatataa ataggttttc agaacaccct
     4321 tgtcaacaac ctttttatat ttgcatgcaa ccccttcccc gtcaaaggat ttggaagcaa
     4381 gacccccttc catatatggg tcgtctatta atgtgaattt atcgcttgct attttttcat
     4441 taagttttcc ttttaataat gaaagattct tttggactgc ctcagctgaa aaaattcctg
     4501 taaatgcttc catcaagtct gcagcagctg aatttctgat aatcacattg taatccccgg
     4561 attttacaga ccttgctcca agcagtgcca atgcttcatc tacggcctcc ctggcaatat
     4621 cctgcgcatt gaatgcatta aagtcattcc ctgtacggta tgccatgcct gtttttatat
     4681 cttccccgtc ttttgcaacc acagctatat aggtgtatgc aatgttgctt ttatcccgga
     4741 ggaaaagtcc cttggtattg gacatgattg tttccccgta accgtcgccg tatatgcaca
     4801 cctcaacaga tgcaattctt ttatccagcc tgaatgcttc ttcctcaaga cttttaacaa
     4861 aattaatttt ttgttcctca cctacatttg acagggcctg attaaaggaa tcaacctttt
     4921 tatattcctt tgagccttca aatatatact cctcatcctc tgaatctatt atgagggcat
     4981 tttccgctgc atcttttacc agctggtcaa tggatgtttc atctaccttt tcagtatatg
     5041 aata
//
LOCUS       DAOPUT010000099         4985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_16936_length_4985_cov_19.113793, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000099 DAOPUT010000000
VERSION     DAOPUT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16936_length_4985_cov_19.113793"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKK75_07390"
     CDS             complement(<1..209)
                     /locus_tag="PKK75_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNZ83461.1"
                     /translation="MNKDIKKILIEEKELQSRVTELAAKITEDYKGKDLLIVCVLKGA
                     VVFASDLIRKIDLPLDIDFMAISSYG"
     gene            complement(202..1593)
                     /gene="tilS"
                     /locus_tag="PKK75_07395"
     CDS             complement(202..1593)
                     /gene="tilS"
                     /locus_tag="PKK75_07395"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNZ83462.1"
                     /translation="MYNLVKNNIINMKLINSGDNILLAFSGGPDSVFLFHNLRKLRET
                     LSFNLYASHINHMYRGKDALHDEEFVRKLCDEYGIRLFVKRKNATEYAKELKVTEEEA
                     GRILRYTFFEDNLKETGGGKVALAHNLNDQAETVLQRIIRGTGIDGLSAMTFQKDNLI
                     RPMLNVSRDEIMNYLHENNYEYCIDITNTQDIYGRNKIRLNLIPYLEKNFNPNIQASL
                     YRMSETMGRDKKIIEKHVEAKYREVFKDKSSSRLVMDLNLLKTLDVGETGRIIRRGIE
                     ELKGNTVNIEMKHIDCAINLINSGKTGKKINLTEGFTIEISYDNFIINKTVEIVSEFE
                     YNIVLNRLNCIKEINKTIYAETADIKEIPEINNKDKDIIIIDCDMVKGSLTARNRRPG
                     DSMIPCGMKGSKKIKDIFIDLKVPKEERESRLIIADDENVIWLEGYRINDKYKVTDKT
                     EKILKISMGWQNE"
     gene            complement(2034..3575)
                     /locus_tag="PKK75_07400"
     CDS             complement(2034..3575)
                     /locus_tag="PKK75_07400"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNZ83463.1"
                     /translation="MIGKAKDKKYASIIFESLKKEFEHIKENWLLLLLCFFISRQNVI
                     HEIFPFAIVVLSAYCHIKGPWISIFALCIAGVLSMAFDFVYIVMLTAIFGYFFSFRNE
                     ERKSIFLAAGYSSAVLFFSKTAILTVDGFNANEFMLIVFETLFIFSAIVLIVEGHKTV
                     TNIKKGRIKQMQGLKIRKKEDTQVIPVTYENAEIEAAPTVSYTADKDKRKNRYLNIFS
                     DEAKKKIKEQLLWQNINVKFFEVISSSKSSILLSMTVKTEKTPEEAEDTVALIVRNIT
                     GARMRCVDKVVMSSNYYVLKFKNTKKIKIHTYSAAAIKDGSLMSGDNFAYAGRQDKYY
                     TVLCDGIGSGEEASSESNGTVDLMSRLLYSDFTEEQILKTLNSVLMIKLGDERYVTFD
                     FIIIDNRTKEIRLYKAGAAPSYILSGKNVDKISGRSLPLGILDNFEYSSFKKEFSIGD
                     MVVMVTDGIIDSINLDPKKSLDKYLERIGEKDPQTIANLILSYALRGQKKIMDDMTVL
                     VTKIG"
     gene            complement(3653..4111)
                     /locus_tag="PKK75_07405"
     CDS             complement(3653..4111)
                     /locus_tag="PKK75_07405"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNZ83464.1"
                     /translation="MNEEQRIIELKKKINHYDFREKEREIKEQKRIQKMTAPIKKKRK
                     FNVINFLFLVFVMYFAYTAFNQYEMLADLNGQIEEKEMIKAETEREAFELKSDVEKLS
                     QEEALMEIVEKIARDQYKMVKPNETIYIDKNKNDNKLIQGIGSEKDLINE"
     gene            complement(4118..4672)
                     /locus_tag="PKK75_07410"
     CDS             complement(4118..4672)
                     /locus_tag="PKK75_07410"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HNZ83465.1"
                     /translation="MDYLPYSQEYMLAVSILAGVFLGFLWDIYRLIRHYGKFKTAGTV
                     IGDILYWLISINLCTRLILDISYGHVRFFILMGFTAGALLYFYGVSRYVLKLFIFIID
                     AVIKVIKTIIHLLIAPVKFIIGKIKIILYPVKLKYIKTKGKLNKRYKFLKFKLKKVSK
                     NRKMIYNRKKRLRRNRRKKGRRKE"
     gene            complement(4674..4946)
                     /gene="yabP"
                     /locus_tag="PKK75_07415"
     CDS             complement(4674..4946)
                     /gene="yabP"
                     /locus_tag="PKK75_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HNZ83466.1"
                     /translation="MDEKIMQSLVLENKEVLNVTGVEGVDNFNDETVVLITSKGRLTI
                     KGENLSISKLNVDEGKLMVKGIINSMVFSEYEGQREKVSLVKKLFK"
BASE COUNT         1417 a         1020 c          698 g         1850 t
ORIGIN      
        1 ccatagcttg aaattgccat aaaatctata tccaaaggca ggtcgatttt tcttattaaa
       61 tcacttgcaa atactaccgc accctttaac acgcaaacaa taagtaaatc cttgcccttg
      121 taatcctcgg ttatttttgc agcaagttca gtcacccttg actgcagctc tttttcttca
      181 atcagtattt ttttaatgtc cttattcatt ttgccacccc attgaaattt ttagtatttt
      241 ttcagtttta tccgtcacct tgtatttatc atttattcta tagccttcaa gccatattac
      301 attttcatca tctgcaatta tcagccttga ttctctttcc tcttttggaa ccttcaagtc
      361 tataaaaata tcctttattt ttttgcttcc cttcatgccg cagggtatca tcgaatcccc
      421 cggtcttctg tttcttgcag tcagactgcc ttttaccata tcacaatcaa tgattattat
      481 atccttgtcc ttattgttta tttccggtat ttcttttata tctgcagttt ctgcatatat
      541 ggttttattt atttctttta tgcagtttaa cctgttcaaa acaatattat attcaaattc
      601 agagactatt tcaactgttt tatttataat aaaattatca tagctgattt caattgtaaa
      661 gccttcagtt aagttgattt ttttaccggt ttttccagag ttaattaaat tgatggcaca
      721 gtcaatatgc ttcatttcta tattaacggt attccccttc aattcctcaa tgcctcgcct
      781 gataattctt cctgtttctc ccacatccaa agtcttcagc aaatttaagt ccataacaag
      841 ccggctgctg gatttatcct taaagacctc tctatatttt gcctccacat gcttctcgat
      901 aatctttttg tctcttccca ttgtttcaga cattctgtaa agtgaagctt gaatattggg
      961 gttaaagttt ttttccaggt agggtattag attaagcctt attttgtttc tgccgtaaat
     1021 atcctgggta tttgtaatgt caatacaata ttcataatta ttttcatgaa ggtaattcat
     1081 tatttcatcc cttgaaacat taagcatggg cctgattaaa ttgtcctttt gaaaggtcat
     1141 ggcgctaaga ccgtctattc ccgtacctct tataatcctc tggagaaccg tttctgcctg
     1201 gtcgtttaaa ttatgggcaa gggcaacctt gcctcccccg gtctctttta aattatcctc
     1261 aaaaaatgta tatcttaaaa ttctgcctgc ttcttcttct gtaaccttta attccttcgc
     1321 atattcagtt gcattttttc tcttcacaaa gagcctaatg ccgtattcat cacagagttt
     1381 tcttacgaac tcctcatcat ggagggcatc tttcccccgg tacatatggt ttatatgaga
     1441 agcataaagg ttaaagctta aagtttccct taactttcta agattatgga acaaaaacac
     1501 cgaatcaggc ccgccggaaa aagccagcag tatattatct ccggaattaa tcaatttcat
     1561 attaataata ttatttttaa ctaaattata catattcttc ctctttgcat atatagcata
     1621 aattatacca caaaaatggt atttgcaata tattttttga aaattaaaaa acgggaagat
     1681 tcccgtcata agcttttcta ttttatgtac atacacatgc ccgttgtttc tttcatatat
     1741 tatttataaa caaataattg gagcaattta agtaaaaaat atatagtata ggggatgtgc
     1801 aaaaatgcaa tcttgtgaac taataactta tatatctgct atagcatgca taatttcaaa
     1861 gtctttttcc aaggaagaac ttcctgtaat cgcctcatta tttactcagt taggggacac
     1921 attaagtaca atcattgcaa atgaagagtt ttgttccaat gacacggaag aataattcca
     1981 tgtccattta ccggtaagct cttatgcttg attgaaacat ttttttaaga atttcatcct
     2041 atttttgtaa ctaaaacagt catatcgtcc attattttct tctgacctct caatgcatag
     2101 cttaatatta agtttgcaat ggtttgaggg tctttctccc caatgcgctc caaatatttg
     2161 tcaagggact ttttaggatc aaggtttatg gagtcaatta ttccgtccgt gaccataacc
     2221 accatatcac ctatgctgaa ctctttttta aatgagctgt attcaaaatt atcaaggatt
     2281 ccaaggggca ggctcctgcc gcttatctta tcgacatttt tgccgcttaa aatatatgaa
     2341 ggcgcagcgc ctgccttgta aagtcttatt tcctttgtcc tgttgtctat tattatgaaa
     2401 tcaaaggtta catatctttc atcgcctaat ttaatcatca gcactgaatt tagcgttttt
     2461 aaaatttgtt cctcggtaaa atcagaatac aagagccttg acatcaagtc aaccgtgccg
     2521 ttgctttcgc tgctggcttc ttcccctgac ccgatgccgt cgcaaaggac agtataatac
     2581 ttgtcttgtc tgcctgcata tgcaaaatta tcaccggaca tcagggatcc gtccttaatg
     2641 gcagctgcac tgtacgtgtg tatctttatt ttcttcgtat ttttaaattt cagcacataa
     2701 taattagacg acattacaac cttatctaca catctcatcc ttgcaccggt aatatttctt
     2761 acaatcaatg caactgtatc ttctgcctcc tcaggtgttt tttcggtttt aaccgtcatg
     2821 cttagcagga ttgacgattt gctgcttgat atgacttcaa aaaattttac attgatattc
     2881 tgccataaaa gctgttcttt aattttcttt ttagcctcat ctgaaaatat attcaaatac
     2941 ctgttcttcc ttttatcttt gtctgctgta tatgaaacag tcggggcagc ttcaatttca
     3001 gcgttttcat atgtgaccgg aataacctga gtatcttctt tcttcctgat ttttaacccc
     3061 tgcatttgtt ttatccggcc cttctttata ttggtgacgg tcttatgccc ctcaacaatt
     3121 aatactatgg cgctgaaaat aaacagtgtt tcaaatacaa tgagcataaa ttcatttgca
     3181 ttaaacccgt caacagttaa aattgcagtc tttgaaaaaa acaatacagc tgaagaatat
     3241 ccggctgcca aaaaaattga ttttctctct tcattccgaa aactgaaaaa gtatccgaaa
     3301 atggctgtaa gcattactat atagacaaaa tcaaaagcca tcgacaatac tccggcaatg
     3361 catagggcaa atattgaaat ccagggacct tttatgtggc agtatgcgct taaaacaaca
     3421 attgcaaatg gaaatatttc gtggattaca ttctgccttg aaataaaaaa acacagcaaa
     3481 agcaaaagcc agttttcttt gatgtgttca aattctttct ttaatgattc aaaaataatt
     3541 gaagcatatt ttttatcctt agcttttcca atcatatttt cacccctcat taattaatgg
     3601 tagcaaatat gattgtaaaa tactgtcaaa atatgcttaa aatcataaaa atttattcat
     3661 ttatcaagtc tttttcagaa cctatccctt gtataagctt attgtcattt ttgtttttat
     3721 ctatgtaaat agtttcattc ggtttaacca tcttgtattg atctcttgct attttttcga
     3781 ctatttccat tagagcttct tcctggctca acttttccac gtcgctttta agctcaaatg
     3841 cctccctttc agtctcggct tttatcattt ccttttcttc tatctggccg ttgagatctg
     3901 caagcatttc atactgattg aatgctgtat aggcaaaata catgacaaat actaaaaaca
     3961 gaaaattaat gacattgaat tttctctttt tctttattgg agcagtcatt ttttgaattc
     4021 ttttttgttc ttttatttct ctctcttttt cacgaaaatc ataatgatta atcttttttt
     4081 tcagctctat tatcctttgt tcttcgttca atcaagacta ctcctttctt ctgccttttt
     4141 tccgtctatt tcgtctcagc cgttttttcc tattatatat catttttcta tttttggaaa
     4201 ccttttttaa tttaaatttt aaaaatttat accttttatt taattttccc ttggttttaa
     4261 tatatttcag cttgacagga tacagtatta tcttgatttt tcctattata aacttaaccg
     4321 gggcaattaa aagatgaatt attgttttta tcacctttat gactgcatca ataataaata
     4381 tgaacagctt taaaacatat ctgctgacac cgtagaaata caaaagagca cctgctgtaa
     4441 atcccatcaa aataaagaac ctgacatggc cgtagctgat gtccaggatc aggcgggtac
     4501 ataaattgat gctgattagc caatatagaa tatcaccgat aacagttccg gctgtcttaa
     4561 acttgccgta atgcctgata agcctgtaaa tgtcccataa aaatccaaga aacacacctg
     4621 ccagtatgga gacagcaagc atgtattcct gggaataagg taaatagtcc atattatttg
     4681 aacaatttct ttactaggct gactttttct ctctgtcctt catattctga gaaaaccata
     4741 gaatttatga tgccctttac cataagcttg ccttcatcca catttaactt gcttatggac
     4801 aggttttccc cctttatggt aagcctgcct tttgacgtta tgagcacaac tgtttcgtcg
     4861 ttaaagttgt caaccccttc aacaccggtg acatttaaaa cttctttatt ttcaagcaca
     4921 agactctgca ttattttttc atccatgaac attcccccct cgaaatatta tatgtccagg
     4981 agatt
//
LOCUS       DAOPUT010000100         4930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_17177_length_4930_cov_17.349538, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000100 DAOPUT010000000
VERSION     DAOPUT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17177_length_4930_cov_17.349538"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..827)
                     /gene="lgt"
                     /locus_tag="PKK75_07420"
     CDS             complement(93..827)
                     /gene="lgt"
                     /locus_tag="PKK75_07420"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNZ83467.1"
                     /translation="MNPVAFTIFGIDVMWYGVLISAGVMLGVLIALKEAKRTGFKEDD
                     LIDFLLYAIPAGIVGARAYYVIFSWDYYSQNLSQIINIRNGGLAIHGAIIAGTITGIL
                     FCRKRRINVLEMLDLVMPSAALGQSIGRWGNFINQEAHGGPTDLPWGIIVNGQKVHPT
                     FLYESIMNFLIFLFLVWFRKNKKAGHGQILGLYLILYSTVRFFIEGLRTDSLMFAGMR
                     VAQLISIASIIIGAALLMYVKKKNTE"
     gene            complement(873..3281)
                     /gene="pheT"
                     /locus_tag="PKK75_07425"
     CDS             complement(873..3281)
                     /gene="pheT"
                     /locus_tag="PKK75_07425"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNZ83468.1"
                     /translation="MLVPIKWMKKYVDIDVDDKTLADKLTLSGSHVDAVIDYKKEVKC
                     VVTGKILEIKQHPNADKLVVAPIDINQGDPLQIITGAKNVKKGDVVPVALEGAELPNG
                     LKIGKTNFRGLASYGMMVSYEELLFEDKVIPKESRDGIIILPPDTPIGKDINEILELD
                     GSALEVEVTFNRPDCLNIVGMARETAATLGTKFKFPKIEIKNEEGNISDYLNGVEIED
                     EDLCTRFYARVVKDIKIGPSPLWMQRTLMDAGMRPINNIVDVTNFVMLELGQPIHAYD
                     LEKLEGRKLVARRAKENEKIITIDQTERALDNSMLVIADGEKPACIAGVMGGYYTEIS
                     DNTKIMALETANFNPKSVRDTSKKLALRSEASARNEKPMGYVMCEIASNRVCQLIEMI
                     GAGTVVGGFLQAGKSEYEPPVVTLRPHRSNSLLGVEVPVADMLNFLNKLEIQSVYDGE
                     KITSMIPYFRTDIEQEADLIEEVGRLYGFEDIESKPLRGSTRVGRKSSLRMVEDEIKS
                     ILNGMGLYEITTYSFISPKAYDSICLEEDSPLRNYVRILNPLGEDFSSMRTTLLPNML
                     EVIQRNSNRGVEAAKLYEAGSIFIPKGQPVTEEPWEKSRLCIGMYGSCDFFDLKGIIF
                     AMLKRFGIKPELKYVTGNPSYHPGRTAAIYVDDDVIGTFGEASPKVTDNYGLDNRVYL
                     AEIDIEKILQHKNTDWKYEPMPKYPSMVRDIAVIVKDEVLAGDMEEEIKKVNPYLIES
                     VELFDVYKGEHITEGYKSTAFSVTYRNKDRTMKDKEVEKIHERILQVLSKKFNAVLRG
                     QGDD"
     gene            complement(3292..4326)
                     /gene="pheS"
                     /locus_tag="PKK75_07430"
     CDS             complement(3292..4326)
                     /gene="pheS"
                     /locus_tag="PKK75_07430"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNZ83469.1"
                     /translation="MKEQLLKLKTEALNRLNECNDNADIEKVRIQYLGKKGELTAILR
                     SMGSLSEEERPEMGKLANEVRAIIEEAILSAKETVHERESEKRLQRETLDITMPGRYP
                     EIGRRHPLMKVKEELESVFMRMGYDVIGGPEIETVANNFDDLNSPYDHPSRDWSDTFY
                     ISDELVLRTHTSPVEIRVMKDAVREGKLPIRMVSTGRTFRFDDVDDTHSPMFHQMECL
                     VVDKGVTMANLKDSIDQFVRELFGSSVKTRFRPHNFPFTEPSAEVDVSCHLCMGKGCP
                     ACNGTGWSMELLGCGMTHPNVLRNCGIDPEIYSGYAFGMGIDRVAMVKYGIPNIRLLF
                     ENDVRFLKQF"
     misc_binding    complement(4597..4823)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1264 a         1154 c          837 g         1675 t
ORIGIN      
        1 gctgggatga caagggcatc ccaaccatag atagaatgaa taaacttaac atacaatgac
       61 tgtcggggac gaatgattcc cgacaggtca ttttactctg tatttttctt ttttacatac
      121 ataaggagag ctgctccgat tattattgat gcaatactta taagctgggc aaccctcatg
      181 cctgcaaaca tcaggctgtc tgtccttaag ccttcaataa aaaacctcac tgttgaatac
      241 aaaatcaggt ataagcctaa tatttgacca tggcctgcct ttttattttt tctaaaccat
      301 accaggaata gaaatatcaa aaaattcatg atggactcat agaggaacgt aggatgaact
      361 ttttgaccgt tcacaattat tccccagggc aggtcagtgg ggcctccatg ggcttcctga
      421 ttaataaaat tcccccatct tcctattgac tggcctaaag cagcgctggg cataaccaga
      481 tcaagcatct ccaatacatt gattcttcgt ttcctgcaaa acagtattcc cgttattgtg
      541 cctgcaatga ttgcaccgtg aattgcaagc ccgccgtttc taatatttat aatctgagat
      601 aagttttggc tgtaataatc ccatgaaaat ataacatagt atgctctggc tccgactatg
      661 cctgcaggta ttgcatataa caggaaatct atcaggtcat cttctttgaa gcctgttctt
      721 ttagcttcct ttaatgctat aaggactcct aacataacac ctgcggatat caatacgcca
      781 taccacatga catcaattcc aaatatagta aaagcaaccg gattcatatt tgttctcctc
      841 ttatgttttt tccgttatat atgtcctgaa tattaatcat ccccttgtcc tctcaggacg
      901 gcgttgaatt tctttgaaag cacttgaagg attctttcgt gaattttttc cacttccttg
      961 tctttcattg tacggtcttt gtttctgtag gtaacagaga aagcagtgct cttatatcct
     1021 tccgtaatat gctcgccttt atacacatca aacagctcaa cactttcaat caaatatgga
     1081 tttacctttt taatttcctc ttccatatcg ccggcaagga cttcgtcctt gactataact
     1141 gctatatccc ttaccataga aggatatttc ggcattggtt catatttcca atctgtattt
     1201 ttatgctgaa gaattttttc tatgtcaatt tcagctaaat aaaccctgtt gtcaagaccg
     1261 taattgtctg taaccttagg ggatgcttct ccgaaagttc ctataacgtc atcgtccacg
     1321 tatattgcag cagttctgcc tggatggtat gaagggttgc cggttacata tttcagttca
     1381 ggcttaatcc cgaatctttt aagcattgca aatataattc cttttaaatc aaagaaatca
     1441 cagctgccgt acattcctat gcacagcctt gatttttccc agggctcctc agtaacaggc
     1501 tgccccttcg gaatgaaaat gcttcctgct tcatataatt ttgcagcttc aactccccgg
     1561 ttggaatttc tttgtattac ctccaacatg tttggcaata gcgtagttct catggatgaa
     1621 aaatcctccc cgagcgggtt taaaatcctc acataatttc tcaaagggct gtcttcttcc
     1681 aggcatatgc tgtcatatgc cttggggctt atgaatgaat aggtggttat ctcatacaat
     1741 cccatgccgt ttaatatgga tttgatttca tcttccacca tgcggaggct ggattttctt
     1801 cccacccttg tgcttccccg caatggtttt gattcaatgt cttcaaatcc atagagtctt
     1861 cctacttctt ctatcaaatc agcttcctgc tcaatatctg ttctgaaata tggaatcatg
     1921 gatgttatct tttctccgtc gtatactgac tgaatctcca gtttgttaag aaaattcagc
     1981 atatcggcta caggtacctc aacacctaag agtgaatttg accggtgggg tctcaaggtt
     2041 accacaggag gctcatattc gcttttccct gcctggagga aaccgcctac aaccgttccg
     2101 gcacctatca tttcaatcag ctggcatact ctgttgctgg caatttcgca cattacatat
     2161 cccataggct tttcgtttcg tgctgaagct tccgaacgca gtgccaattt tttggatgtg
     2221 tctcttacgg atttaggatt aaagtttgct gtttccagtg ccataatttt tgtattgtcg
     2281 ctgatttcag tataatatcc acccataact ccggctatgc aggcaggttt ttctccgtct
     2341 gcaattacaa gcatactgtt gtccaaagct ctttcagttt ggtcgatagt aataattttt
     2401 tcattctctt ttgctcttct tgcaacaagc tttctgcctt ccaatttttc cagatcataa
     2461 gcatgaatag gctggcccag ctcaagcatt acgaagttgg taacatccac tatgttgttt
     2521 attggtctca tccctgcatc catgagagtt ctctgcatcc acaagggtga gggtcctatt
     2581 ttaatatctt tgactaccct tgcataaaac cttgtgcaca ggtcttcatc ttcaatttca
     2641 actccgttta aataatccga aatatttcct tcttcatttt taatctcgat ttttgggaat
     2701 ttaaattttg ttcccaatgt ggctgcagtt tcccttgcca ttcccacaat gttaagacag
     2761 tcaggcctgt tgaatgtaac ttcaacctcc agggcgcttc cgtcaagctc aagaatttca
     2821 tttatatctt tacctatggg agtgtcagga ggcagtataa ttattccgtc tcttgactcc
     2881 ttgggtataa ccttgtcttc aaataacaat tcctcatatg aaaccatcat cccgtaagat
     2941 gccaatcccc taaaattagt tttaccgatt tttaacccgt taggcagttc agcaccttcc
     3001 agggctacag gcaccacgtc gcccttcttc acattcttgg ctccggtgat aatctgcaaa
     3061 gggtctcctt gatttatatc tatgggtgca acaaccaatt tgtcggcatt tggatgctgt
     3121 tttatttcaa ggatttttcc tgtaacgaca cactttactt ccttcttata atcaattaca
     3181 gcatccacat gtgaccctga tagagtcaat ttgtctgcca gtgttttgtc atcaacatct
     3241 atgtccacat atttcttcat ccatttaata ggtactaaca tagaaaacct cctaaaattg
     3301 ttttaagaat ctaacatcat tttcaaataa gagcctgatg ttggggattc catacttaac
     3361 cattgcaacc ctgtcgattc ccataccgaa tgcatatccg gagtatattt cagggtctat
     3421 gccgcagttt ctgagaacat tcgggtgagt cattccgcag cccaaaagct ccatgctcca
     3481 tcctgttccg ttgcatgcgg gacatccctt acccatgcac aagtgacagg atacgtcgac
     3541 ttctgcactt ggctccgtga atgggaagtt gtgaggtcta aaacgggtct tcacgctgct
     3601 tccgaacaat tccctgacga actggtcaat tgaatcctta aggtttgcca ttgtaactcc
     3661 tttatctaca accaggcact ccatttgatg gaacattggt gaatgtgtat cgtccacatc
     3721 gtcaaagcgg aatgttcttc ctgtggaaac catacgaatt ggaagtttcc cttccctcac
     3781 agcatccttc ataaccctta tttccacagg tgacgtatgt gtcctgagta ccagctcatc
     3841 tgaaatataa aaagtgtctg accagtctct tgaaggatgg tcataaggtg agtttaaatc
     3901 atcaaagttg tttgcaactg tttctatttc cgggccgccg attacatcat atcccattcg
     3961 cataaaaaca ctttccagtt cctctttaac cttcatcagt ggatgtcttc ttcctatttc
     4021 aggatatctc ccaggcattg ttatgtccag ggtttctctt tgaagtcttt tttctgattc
     4081 cctttcatgt actgtctctt tggctgatag gattgcttct tctataattg ctcttacttc
     4141 attggccagc ttccccattt ccggcctttc ttcctctgac agagagccca ttgaccttaa
     4201 aattgcagtc aattcacctt ttttgcctaa atactgtata cggactttct caatatcagc
     4261 attgtcattg cattcattca accggttcaa tgcttcggtt ttcaatttta ataattgctc
     4321 tttcaaagtt tcccttcttt cttggatatt ttctgaataa tattcatata tttagtaaag
     4381 ttactttttc gtaattttac atacgcaggt tccatcacca atttgttcat tccctctggt
     4441 catgcaaatt gggattgcgc tgcataagtc ctaccgctat ttttcagaat gtcatattcg
     4501 gggacattcc tctaactcca aagactatcc tgataataga ggtgtgtccc catactttta
     4561 aaaaataggg ttagggctat aaaaaaactc cccctcataa gaggcgaagt ccgcggtacc
     4621 actctttttc acttttgaat atcaatcttg actttcaaaa gtcttgtttg tataacggtt
     4681 ttcccgtgca aaactacttg ttttcatctt gcaggctcca aagcgaaaat tcggtatagg
     4741 tgcctaaaac cacttacagc ccatgatggt ttttctctaa aaggcatttg tttatactta
     4801 ctactcttta tcattgccaa tatttaatta gtgttaagcc atcccgcagc ctagctgcgc
     4861 atcttttaca gattatacta tataggaaat tttatttcaa caactttttt ctaatttcgt
     4921 atatacttat
//
LOCUS       DAOPUT010000101         4906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_17297_length_4906_cov_21.276644, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000101 DAOPUT010000000
VERSION     DAOPUT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17297_length_4906_cov_21.276644"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1021)
                     /gene="ytxC"
                     /locus_tag="PKK75_07435"
     CDS             complement(185..1021)
                     /gene="ytxC"
                     /locus_tag="PKK75_07435"
                     /inference="COORDINATES: protein motif:HMM:NF020391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YtxC"
                     /protein_id="HNZ83470.1"
                     /translation="MELLSLELNESENHEIYNNIKIITNLDESEINIELSSTDEGNIK
                     IRYFTDNKIKKREYIEKIIGKITKLLVEYTKLMGINALKENYFYFDEDEVGSIISDIE
                     EEIDNDIKIHIIIKNKFKEVLENSSLINLNGFVNFRLKFLKLYATQVVERCIDSYLMK
                     KEYMDFISIIKLISDSGENQYDVVNVMFNNNKFQVYDKNMQKLNYFSNVEFSAELNTE
                     STYDETVINILMSVSPKKIILHDLKVDKKNKEAYNTADIVKRIFEGKVEICPGCKYCE
                     FL"
     gene            complement(1049..1783)
                     /locus_tag="PKK75_07440"
     CDS             complement(1049..1783)
                     /locus_tag="PKK75_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83471.1"
                     /translation="MNPYIPFKNPRCVIIDYRASDEILNYIKKLNIEPIKSVKCPELH
                     EPVDGHPDMVIHPIDFETFVIAPNVYDYYRNVLKSLGIKVIKGGKTLNRNHPEDIAYN
                     VARIGRYAVHNTKHTDQKLKYYLEEAGIEFIHVNQGYTKCSIAPVGENKALTSDFLIH
                     EKLISYNIDCMYIDPKVVYLQGYDNGFIGGSLGLINDKIFLSTGKIFDEDISLSLRNF
                     IKAAGYIYDEASNQQIIDLGTLIPVI"
     gene            complement(1863..2051)
                     /gene="thiS"
                     /locus_tag="PKK75_07445"
     CDS             complement(1863..2051)
                     /gene="thiS"
                     /locus_tag="PKK75_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNZ83472.1"
                     /translation="MNNRDFPWEEGLTIEKIMEIKKYTFSKIIVKVNGNHIEKEEYAS
                     TIVNDGDVVEMIHLLAGG"
     gene            complement(2146..4287)
                     /locus_tag="PKK75_07450"
     CDS             complement(2146..4287)
                     /locus_tag="PKK75_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ83473.1"
                     /translation="MKENNKVLAQYEYKPGEIDKGYTNRTLYVNISDNTISPKPVTQM
                     MKEKFIGGKGFGLWYLWNATTPNTKWDDPENEIIIAGGPACGITQYAGTGKSLVCSIS
                     PLTDIPIDSNVGGYFGPLLKFSGWDAIEIQGKSDKEIIVYIDGNKSLVTIEEAPAGAF
                     DAHILGEQLTEMYADNEEDKRNVAVVTAGTGAEHSRFGILNFTFYDPRRQTARLKQAG
                     RGGIGTVFRNKKIKAVIVKFSSVKGNLNNAFDQSIINKTGIKYHKEIHDLDHMQNNMR
                     KIGTANIIEVMDAYDLLPVMNFQYGSHPDTQKIKSTVFIEKYLTQGVQDGCWYGCSMA
                     CAKAADNFLIKTGPYKGHRVTVDGPEYENAAGLGANCAIFDPEGILELNFYCDTYGID
                     TISFGTATAFAMECYERGIINKDITGGLELNFGNFYSSVELLHQMSKGEGFGTVVGQG
                     VHIMKRLFAEKYGADKDFLFDIGMEQKGLEYSEYGSKESLAQQGGYGLTNKGPQHDEA
                     WLIFMDMVNNQIPTFEDKAEALHYFPMFRTWFGLYGFCKLPWNDIVPADNKYTDEPAK
                     VPEHVQNYLDVVYGVTGKRLTSDEMVKESERVYNFQKIFNIRMGKGLRNDDKVPYRSM
                     GPVTREEYESRQERYDKQLKEKIGYNIEGKTTEEKMAVLREYREEQYEKLIDAVYKRR
                     GWNSNGVPTLDHLKYLDMELPELIEVVKDLQ"
     gene            complement(4297..4725)
                     /locus_tag="PKK75_07455"
     CDS             complement(4297..4725)
                     /locus_tag="PKK75_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ83474.1"
                     /translation="MKYLAKDESKCIQCGTCESICSKTYFKEDNPDKSCIQINKDGDP
                     EINVCNQCGECIDICPVMAIKRDKQGVVRINKKICVGCFMCVGFCPQLSMRQHDDYIE
                     PFKCIACGLCAEECPTGAIYIAERSDDEAREMPLDKVLNR"
BASE COUNT         1323 a         1003 c          737 g         1843 t
ORIGIN      
        1 gttattgtta ttaatgctgt taaaatcaaa aatggcatat gtatcctcct tttctaatac
       61 tataatcaat aaaccttgaa gtttccttaa acttttctta ttaagctatg taaccgggga
      121 catctctcga cctcagaaac taaccttaaa gaagaggtgt gtccccatat ccgttagggc
      181 atctctataa aaactcgcag tacttgcagc cgggacagat ttcgaccttt ccctcaaata
      241 tcctctttac tatatctgca gtattatatg cttctttatt tttcttatca acctttaaat
      301 catgaagtat tatttttttc ggcgacacgc tcatcaaaat atttattaca gtttcgtcat
      361 acgttgattc agtgttcaat tcagccgaaa attccacatt tgaaaaatag ttaagtttct
      421 gcatgttttt atcgtaaacc tggaacttat tgttgttaaa cattacattt acaacatcat
      481 attgattttc tccggaatcc gatatcagtt taataatact gatgaaatcc atgtattcct
      541 tcttcattaa atatgagtca atacaccttt ccaccacctg tgtcgcgtaa agcttcaaaa
      601 acttaagcct gaagtttaca aatccgttta aatttattaa gctcgaattt tccaatactt
      661 ccttgaactt atttttaata ataatgtgta ttttaatatc attgtctatt tcttcctcaa
      721 tatcggatat tattgaaccg acttcatcct cgtcaaaata aaaataattt tccttcaggg
      781 catttatacc cattagttta gtgtattcaa ccagaagctt tgttattttc cctattattt
      841 tttcaatgta ctctcttttt tttattttat tgtccgtaaa atatcttatt tttatattac
      901 cttcgtctgt agatgataat tctatattta tttcgctttc atccagattt gttattatct
      961 ttatattatt atatatttca tgattttcac tttcatttaa ctctaatgat aatagctcca
     1021 cttagtcact cctctcggtc aagggaggtc atattaccgg tatcagcgtt cctaagtcta
     1081 taatctgctg atttgaagct tcatcataaa tatatcctgc tgccttgata aagtttctta
     1141 atgaaagaga aatatcttca tcaaatattt tacctgttga caaaaatatc ttatcattaa
     1201 ttaaacctaa agagcctcct ataaatccgt tatcgtatcc ttgcaaatat acaacctttg
     1261 gatctatata catgcagtct atattgtatg atataagttt ttcatgtatc aagaaatctg
     1321 atgtcaaagc cttattttca cctacagggg caattgagca ctttgtatag ccttgattta
     1381 catgaatgaa ttcgatgccc gcttcttcca aataatattt taatttctgg tctgtatgct
     1441 ttgtgttatg cacagcatat ctccctattc ttgctacatt atatgctata tcttcaggat
     1501 ggtttctgtt tagagttttt ccacctttaa taacttttat tcctaagctt tttagaacat
     1561 ttctgtagta atcgtaaaca ttgggagcaa ttacaaaagt ttcaaaatct atgggatgaa
     1621 ttaccatgtc cggatggccg tccacaggtt catgcagttc agggcatttg acggatttta
     1681 tgggctcaat atttaatttc ttaatataat ttaatatttc atcgcttgct ctgtagtcaa
     1741 taattacaca gcggggattt ttaaaaggta tgtacggatt catattttct cctgtaaaat
     1801 aaataaaatg atataatctt ttaaaattat atcattttgg tatgtgatct acaaatatct
     1861 ttttaacccc ctgccaaaag gtgtatcatt tccacaacat caccatcatt aacaatggtt
     1921 gatgcgtatt cttccttttc aatatggttt ccgtttactt ttactatgat ttttgaaaag
     1981 gtgtattttt ttatttccat aattttttct atggttaaac cttcctccca ggggaaatct
     2041 ctgttgttca gtttaataat ttataccacc tcatttgtta cagggggacg ggggaactgt
     2101 cataatttca cgggatgtca atacccccgt ccccttgtca tattcctatt gcaagtcttt
     2161 tactacttct attaattcag gcagttccat atccaaatat tttaaatgat ccaatgtggg
     2221 gacaccgttc gaattccagc ctcttctttt ataaactgca tctataagct tttcatattg
     2281 ctcttccctg tattctctca atacagccat tttttcttcg gtagtcttgc cttcaatgtt
     2341 gtaaccgatt ttttctttaa gctgtttatc gtatctttcc tgtcttgatt catattcttc
     2401 cctggttacg ggacccatgg aacggtatgg aaccttatca tcgtttctta aacccttccc
     2461 cattcgtata ttaaagattt tctggaagtt atatactctc tcagattctt ttaccatttc
     2521 atcagaggtt aatctctttc ctgtaacgcc atataccaca tcaagataat tttgaacatg
     2581 ctccggcacc tttgcgggct catcggtgta tttattgtct gcaggaacta tgtcgttcca
     2641 gggaagcttg caaaaaccat acagtccgaa ccaggtacga aacataggga agtaatgaag
     2701 agcttctgct ttatcttcaa atgttggtat ctggttgttt accatgtcca taaaaatcag
     2761 ccatgcctca tcatgctgag gccccttgtt tgtcaggccg tatcctcctt gctgggcaag
     2821 ggattctttg gagccgtatt ccgaatattc cagacctttc tgctccattc ctatatcaaa
     2881 caagaagtct ttatcggctc cgtatttttc tgcgaaaagt cttttcatta tgtgaactcc
     2941 ctggcctact actgttccaa agccttctcc tttggacatt tggtggagta gttctactga
     3001 tgagtagaag tttccaaaat tcagttcaag accgcctgta atgtccttgt tgattatgcc
     3061 tctttcatag cattccattg caaaagctgt tgcagttcca aaggaaattg tatctatgcc
     3121 ataggtatcg cagtaaaagt ttaattccaa aatcccttct gggtcaaaaa ttgcacagtt
     3181 tgctcctaat cctgcagcat tttcatattc gggaccatct acggtgactc tatgtccctt
     3241 gtagggacct gtttttataa ggaagttatc tgcagccttg gcacaggcca tggagcatcc
     3301 gtaccagcat ccatcctgaa ctccctgggt gagatatttt tctataaata cagtggactt
     3361 tattttttgc gtatcaggat ggcttccata ttgaaaattc ataacaggaa gcaggtcata
     3421 agcatccatt acttcaataa tatttgctgt tccgattttc cgcatattgt tttgcatatg
     3481 gtcaaggtca tggatttcct tatgatattt tattccggtt ttatttatta tggactgatc
     3541 aaatgcattg tttaaattac cttttacact gctgaatttt acaataactg ccttgatttt
     3601 tttatttctg aatactgttc ctattccgcc cctgcctgct tgtttaagcc ttgcggtttg
     3661 tcttctaggg tcatagaagg tgaaatttaa aattccaaat ctggaatgct cagcacctgt
     3721 tcctgcagtt accacagcaa catttctttt gtcctcttca ttatccgcat acatttctgt
     3781 aagctgctct cccaggatgt gagcatcaaa agctcctgcc ggtgcttctt caattgttac
     3841 aagactcttg ttcccgtcta tatatacaat tatttccttg tctgatttgc cctgtatttc
     3901 aatggcatcc catcctgaaa atttaaggag ggggccgaaa tatcctccaa catttgaatc
     3961 tatgggaata tccgtcaagg gtgaaatgct gcataccaat gattttccag tgcctgcata
     4021 ttgagttatc ccgcaggcag gacctcctgc aataattatt tcattttccg ggtcgtccca
     4081 ctttgtattt ggggttgttg cattccaaag ataccacaaa ccaaaacctt ttccgcctat
     4141 gaatttttct ttcatcattt gagtcacagg cttggggctt atagtattat ctgaaatatt
     4201 tacatataat gttctatttg tatatccttt atctatttct ccaggtttat attcatattg
     4261 agctaacact ttattatttt ctttcatatc ctactcctat ctgtttagaa ctttgtctaa
     4321 aggcatctct cttgcttcat catcgcttct ttctgcaata tatatagcac ctgtagggca
     4381 ttcttccgca cataagccgc aggcaatgca tttaaacggc tcaatataat catcatgctg
     4441 tctcatggat aattgagggc agaagcccac gcacataaag cagcccacac aaattttttt
     4501 gttgattctg actactccct gcttgtctct ttttattgcc ataacagggc aaatgtctat
     4561 gcattcaccg cattgattgc agacattaat ttccgggtca ccgtctttgt ttatttgaat
     4621 gcaggattta tccggattat cttctttaaa atatgtttta gagcatatac tttcacatgt
     4681 accgcattga atgcatttgc tttcatcttt agccaaatac ttcataaaaa cctcctgttt
     4741 tttagtcatc ctaagcagaa tatcagaatg ataggggctt aaccctcaac tattattgat
     4801 tatattatat aaataatttt aattaatttc aacttcttat agtaatatta atatgtttat
     4861 ttttttctat tagctattag ttatttgtaa ataaaaataa cttaag
//
LOCUS       DAOPUT010000102         4776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_17932_length_4776_cov_18.580809, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000102 DAOPUT010000000
VERSION     DAOPUT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17932_length_4776_cov_18.580809"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1830)
                     /locus_tag="PKK75_07460"
     CDS             complement(52..1830)
                     /locus_tag="PKK75_07460"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNZ83475.1"
                     /translation="MKKLTSNSKVLNWVDEIISMTNPANVVWIDGSEELYEELRKEGC
                     STGELIKLNEEIYPGCYYHRSAKDDVARMEDKTFICTRKREDAGNTNNWKSPEEAYNL
                     GREIFTDSMKGRTMYVIPYSMGPVGSPFSKIGFEITDSIYVVLSMNIMTRVGQDVLDA
                     LGDSNDFVRGVHGKCTLDINKRYIFHFPEDNTIWSCNSGYGGNVLLGKKCFALRIASY
                     QGRMEGWMAEHMLIVGIEDPQGQVKYIAGAFPSACGKTNLAMLIPPEIYLNKGYKVWT
                     VGDDIAWLRVKEDGRLWAVNPEAGFFGVAPGTNAKSNYNALMSTKKNSIFTNVVYNLD
                     DDTIWWEKLDKNPPENALDWMGNPWNGKTSKENGAHPNSRFTAPAKNCPCISPEWESP
                     EGVPLSAILFGGRRAKTAPLVYQSRDWQHGTFVGSTLASETTAAAAGKVGVVRRDPMA
                     MIPFCGYDMGNYFKHWLDMGRKMENPPKIFHVNWFRTNEKGEFIWPGFGDNFRVIEWM
                     LKRCENKVDANEAPIGYMPKADDINIEGLDISKDTLESLLTIDNESWLEDVKSIEEFY
                     KKIGDTIPDELYEELKTLTGNLNKNK"
     gene            complement(2003..3361)
                     /locus_tag="PKK75_07465"
     CDS             complement(2003..3361)
                     /locus_tag="PKK75_07465"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNZ83476.1"
                     /translation="MDRELVLKPIAVMIICLIFIVGPIVLSIPLYIGFLAGIIFTSLV
                     LYKSGYPISDLWETSLSALKDVKRLLIIILFIGATTSIWLASGVVPTIMYYGFTYMEG
                     VNFLLAAFVIMCIVSYFMGTAVGALGTVGISLSGIGLSIGIPHSLMVGALVSGAFIAD
                     KISPLSGLLNLAMTTFSRNYKEVFKSMIITLIPTIVITSIIYFFIGSNYIARTENLAV
                     YKQAIYEGFNISPVLFILPAIVLILSISGTDSLKTFSVGIISGGALAVIFQKAALSSI
                     IRALIFGYRGNTPSKQLNSLLVSGGMISMIETIFIVAGAFLLIKLFEKGNVLVPLTDK
                     LIHGINSRISLIKRTGFVSILMTVITCDQTSGIIMPGTLLLEKYKEMKLDNAVLARTI
                     FDTGVIVAPLMPWNINSFLIKPIMGITAAQYAPYAVLCYICPLVMMIVSFMQYKDGHK
                     NN"
     assembly_gap    3421..3520
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3521..4363)
                     /gene="rsmI"
                     /locus_tag="PKK75_07470"
     CDS             complement(<3521..4363)
                     /gene="rsmI"
                     /locus_tag="PKK75_07470"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNZ83477.1"
                     /translation="MSKLYVVPTPIGNLGDMTQRAVETLNDCDLILAEDTRHTQKLLN
                     HFNIKTKMISYHKFNEKQRSEEIIQKLLNENINAALVTDAGTPCISDPGYELVREVRE
                     KGIEVIGLTGASALTTALSISGIETYEFAFYGFLPRKKNELDNALKRVVNNPVRTFVF
                     YESPRRILNLIENIKVHMPNSTLCVCKELTKIHEKTFYGNIDEVLDQLKNNDTLEKGE
                     YVVIGYNNDYQEQKTLDKGISIHGILFDKMINNEYSLKEAVKEAAEEGLASKNEAYKA
                     SIEIK"
     gene            complement(4548..4630)
                     /locus_tag="PKK75_07475"
     tRNA            complement(4548..4630)
                     /locus_tag="PKK75_07475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4595..4597),aa:Leu,seq:caa)
BASE COUNT         1297 a          969 c          812 g         1598 t
ORIGIN      
        1 aatgtcccct ttttagattg cttcagctat cttggcaatc agctgtaatg cttacttatt
       61 cttatttaaa ttgccggtta atgtttttaa ctcttcatac agttcatcgg gtattgtatc
      121 cccgattttt ttgtagaatt cctctatgct ctttacatct tcaagccatg attcattgtc
      181 tatggtcaaa agggattcca aggtgtcttt tgatatatcc aggccttcga tatttatatc
      241 gtcagctttc ggcatatagc ctataggagc ttcatttgca tccactttat tttcacatct
      301 tttgagcatc cattcaatta ctctgaaatt atcaccaaag cccggccaga tgaattctcc
      361 cttttcattt gttctaaacc agtttacatg gaagattttt ggaggatttt ccattttcct
      421 gcccatatca agccaatgct tgaagtaatt tcccatatcg tatccgcaga agggaatcat
      481 agccattggg tctcttctta caacccctac ctttcccgct gctgccgcgg tagtttccga
      541 tgcaagtgtg gagcctacaa aagtaccatg ctgccagtct cttgactgat aaacaagggg
      601 agctgtcttt gcacgtcttc cgccgaacag tattgccgat aagggaactc cttccgggga
      661 ttcccattcg ggcgatatgc atgggcaatt cttagcaggt gccgtaaatc ttgagttggg
      721 gtgagctccg ttttcctttg atgttttccc gttccagggg ttccccatcc agtccagggc
      781 attttcggga ggatttttat caagcttttc ccaccatatc gtgtcatcat ccagattgta
      841 tacaacattt gtaaatattg aatttttctt tgtggacata agagcattgt aatttgattt
      901 tgcatttgtc cccggtgcaa ctccgaaaaa gcctgcttcc ggattaacag cccatagtct
      961 tccgtcttcc ttaacgcgta gccatgctat atcatcacct acagtccaaa ccttatatcc
     1021 tttgttcaga taaatttcag ggggtataag cattgccaga ttagttttgc cgcaggctga
     1081 cgggaatgcc ccggctatgt atttcacctg accctgagga tcttcaattc ctactatgag
     1141 catgtgttca gccatccagc cttccatcct gccttgatat gatgcaatgc ggagggcgaa
     1201 gcattttttg ccaaggagaa cattgcctcc gtaaccggag ttgcatgacc atatagtatt
     1261 gtcttcggga aaatggaaaa tgtacctttt gttgatatcc aaagtgcact ttccatggac
     1321 gcctcttaca aaatcattgg aatctcctag cgcatccagt acatcttgtc caactctggt
     1381 cattatgttc atgcttaata caacataaat cgaatctgtg atttcaaaac ctatctttga
     1441 aaagggtgag cccacaggtc ccatagaata tggaataaca tacattgttc tacccttcat
     1501 ggaatcagtg aaaatttccc tccccaaatt gtatgcttct tcagggctct tccaattgtt
     1561 tgtattgcct gcatcttctc tttttcttgt acaaatgaat gttttgtctt ccattcttgc
     1621 tacgtcgtct tttgcagacc tgtgataata gcatccggga taaatttcct cgttcaattt
     1681 tataagttcg cctgttgagc atccttcttt gcgaagctct tcgtacagct cttcactgcc
     1741 gtcaatccaa actacatttg ctggattagt catagaaata atttcgtcta cccaatttaa
     1801 aacttttgag tttgatgtta attttttcat acgccctcct aataatattt atcttatttt
     1861 tattatatta tggctttaaa aaacattcaa taataaatat aagttctttt ttccctaacc
     1921 tttgcagcat tatgccttat ctaattaggt gattgtttgg tttgacttat tttactattg
     1981 aagtaaatac cattgtaaaa tgttaattat ttttatggcc gtccttatat tgcataaatg
     2041 aaactatcat cataaccagt ggacaaatat agcacaaaac tgcataggga gcgtattggg
     2101 ctgctgttat acccataatg ggttttatta aaaatgagtt tatattccat ggcataagag
     2161 gtgctacaat cactccggta tcaaatatgg ttcttgccaa cacagcatta tccagcttca
     2221 tctccttgta tttttccagc aggagggttc ccggcataat aattcccgat gtttggtcac
     2281 atgttataac tgtcatcaat atgcttacga aaccagtcct ttttattaaa gagattctac
     2341 tgttaattcc atgtataagc ttgtctgtta aaggcacaag aacattgcct ttttcaaata
     2401 gtttaatcag caaaaatgct ccagccacta tgaatatagt ttcaatcatg gatatcatgc
     2461 ccccgctgac caaaaggctg tttaactgtt tggagggagt attgcctctg tagccgaata
     2521 tcagagccct tattattgat gaaagcgcgg ctttttgaaa tattacggca agtgctccgc
     2581 ctgatataat tccaacagag aaagttttta aggagtcagt ccctgatatg gataaaatta
     2641 aaacaatggc aggcagtatg aataatacgg gggatatatt aaatccttca tatattgcct
     2701 gtttgtacac tgccaggttc tctgtccggg caatataatt tgaccctatg aaaaaatata
     2761 ttattgatgt aattactatg gttggtatta aagttataat catgcttttg aatacttctt
     2821 tatagtttct gctgaaggtt gtcatggcaa gatttaaaag tcccgacaag ggcgatatct
     2881 tatctgctat gaatgctccg gaaaccagtg ctccaaccat caaactatga ggtataccta
     2941 tgcttaagcc gattccgctt aaagatatgc cgactgtacc taaagctccc acggcagtac
     3001 ccataaaata cgaaacaatg cacattatta caaatgctgc cagaagaaag tttactccct
     3061 ccatataggt gaagccgtag tacataattg tgggtacaac accggaagca agccatatgg
     3121 aagtagttgc gcctataaac aatattatga taagcaatcg ctttacatcc tttagtgcgg
     3181 ataaggatgt ttcccaaagg tcgcttatgg ggtatccgct tttataaagt accagggagg
     3241 taaaaatgat tcctgccaaa aaaccaatgt ataagggaat agataaaact attggtccaa
     3301 cgataaaaat cagacatata atcattactg ctattggttt tagcactaat tctctatcca
     3361 aattttcacc tcatcagaaa aataaatagt agattatggg tataaatatt gagggcagca
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttttatttca atagaagcct
     3541 tatatgcttc atttttggaa gcaagcccct cttctgccgc ctcttttaca gcttccttta
     3601 aagaatattc attgttaatc attttgtcaa acaaaattcc gtggatgctt attcccttat
     3661 ctagtgtttt ttgttcctga taatcattgt tgtaccctat tacaacatat tctccctttt
     3721 ccagtgtatc gttatttttc aactggtcca aaacttcatc tatattgccg taaaaggttt
     3781 tttcatggat ttttgtcagt tccttgcaga cacatagggt tgaatttggc atatgaacct
     3841 ttatgttttc aattaaattc aagattctcc tgggagattc ataaaaaaca aaagtccgga
     3901 caggattgtt aaccactctt tttaaagcat tgtccaattc atttttctta cggggcaaaa
     3961 atccgtaaaa tgcaaattcg taggtttcaa taccagagat ggaaagggct gtggtaagtg
     4021 ctgatgcacc ggtcaagcct attacctcaa tacccttttc ccttacttcc ctgactaatt
     4081 catagcccgg gtccgaaata cagggtgtgc cggcatcagt aaccaaggct gcattaatat
     4141 tttcatttaa gagcttttgt attatttcct cgcttctttg tttttcattg aacttatggt
     4201 atgaaatcat ttttgttttg atattgaagt gatttaaaag cttttgagta tgcctggtat
     4261 cttcggccaa tattaaatca caatcattca gtgtttctac ggctctctgt gtcatgtcgc
     4321 ccagattccc tataggtgta ggcactacat ataatttgct catttttctt atacccttta
     4381 tccatattta gaaaattata acacacattt ttataaatat atagttaaaa gtagaatata
     4441 ggtattatat ttgtcagctt ttaaattctt atgtaattat tcataagccc gactgatatt
     4501 gagtagaata aaaaaacgac tcaataaggt cgtttttttc ggtattatgc cgcagaaggg
     4561 acttgaaccc ccacggtgtt gccaccaacg gattttgagt ccgtcacgtc tgccattcca
     4621 tcactgcggc aaattgctac tttaagatag tagcataaac aaaagtaaaa tgcaaggatt
     4681 tttttattat ttatacttcg ctgaattcta cctctatttc ctcaatttca ttctcacagt
     4741 tcatttcgac cttggatatt ctatcgaatt ttcttt
//
LOCUS       DAOPUT010000103         4716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_18249_length_4716_cov_20.177001, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000103 DAOPUT010000000
VERSION     DAOPUT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18249_length_4716_cov_20.177001"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..628
                     /locus_tag="PKK75_07480"
     CDS             92..628
                     /locus_tag="PKK75_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83478.1"
                     /translation="MRYYPFDDRTYKQRNKNKMFTLNQAPPAETPQPPVPPQPPAVTQ
                     PKLDTGNLSVSVFTASGALPVPDALVTVYTHDESGVENEIERHVTDANGQIPLITLPV
                     IYDPFNPLESSEYFFTTYNLRVQAINYYTVNIIDLRIFPNTTTSYQIDMIPAAIGPVK
                     GPDTTIVIPPSPVDISND"
     gene            641..2035
                     /locus_tag="PKK75_07485"
     CDS             641..2035
                     /locus_tag="PKK75_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNZ83479.1"
                     /translation="MSIGIPGLTEVVIPDSITVHLGAPDQPAENITVPFLDYIKNVAS
                     SEIYPTWPESALRANIYAITSIALNRVFTEWYRSQGYNFDITSDTRFDQAFVNNRGIF
                     DNVSALVDEIFDSYIVRQGQIEPLYAQFCDGRVSQCPGLLQWGTVELAKEGLTPYEML
                     QYYYGANINIQTDTPVAAAFETYPGINLKPGDNNIYILLMQIYLNTIANNYPAITRIP
                     NPTGTFDETTQAAVSQFQRIFNLPVTGIIDKATWYRIRRIYVAVTSLAGLTSQGVVLS
                     EIPQYTPTPGPEEVVPRVQAVQYFLNVLSAYYNGIPSVDINGVLDTRTRTAIMEFQRI
                     FNLPITGLVDEQTWYTMYDAVSGILRTLPPSAIALPAILWPGITYQLGSEGPGVYFIQ
                     QYLSYIASVLEGIPPAAPDGIYGPETERAVRTFQEYFGIDVTGVVDRYTWGRIVLIYR
                     NLRFGNTGNIGGLQ"
     gene            complement(2139..2714)
                     /locus_tag="PKK75_07490"
     CDS             complement(2139..2714)
                     /locus_tag="PKK75_07490"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNZ83480.1"
                     /translation="MAIKIFIDQGHNPTGFHNAGAVGNGLNESEINYRAGILLANLLA
                     NDYRFEYRLSRPIATTVLGTNTASSLAERVRMANEWPANYFISLHSNANVNPAINGSE
                     MYIYQLNTQANWLAQHIMNGIVRNTRLRDNGIRENRSLYVLRNTNMPAVLVEMGYLTN
                     YNDAMILRDNLWNVAYGIYLGLLSYFGLMPL"
     gene            complement(2858..3949)
                     /locus_tag="PKK75_07495"
     CDS             complement(2858..3949)
                     /locus_tag="PKK75_07495"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNZ83481.1"
                     /translation="MKYICSKCGLTMDAATGKSRCSCGGLWKLDYKSPGFDLSLVDRD
                     NWSIFRYRAFMPLPGNEWENISLGEGLTPVIQLNEDVLLKMDYFMPTLSFKDRGAAVL
                     IAHCKAIGVDSVVQDSSGNAGNSVAAYCAKAGIDCEIFVPEGTSPKKIDMILSHKAKV
                     NVVPGSRDHCADVCRAKADKEGKYYANHVFNPFFYEGTKTYIYEVYEQLKRIPQNIFI
                     PLGNGTLFIGIIKALEEFIESGIIDKMPHIIAVQSECCDPFVRAAALKEKRPVKVTPV
                     PTMAEGIAIGEPMRGEEILGYIYKYNIEVITAPENRILEARAMLAAKGIYIEHTTAAT
                     YAAYMKYCELNGRTPALIPMCGAGLKSDH"
     gene            complement(4009..4590)
                     /locus_tag="PKK75_07500"
     CDS             complement(4009..4590)
                     /locus_tag="PKK75_07500"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNZ83482.1"
                     /translation="MDLMEMLSSQLGNENVLKQLSKSTGADSSQIQQAMKLGLPALMQ
                     AMGKNASTAEGASSLAKALDFHKDDETDNIEDFLKNVNKEDGSKILNHILGSKNKSIQ
                     RNLAKQTGMKADQTTDLMAQLAPLLLGTLGKQKASQKVDSSGLARMLSGALGQGKDNN
                     LMDSVSKLLDADDDGSIVDDITGMLGGFLKKKK"
BASE COUNT         1328 a         1053 c          868 g         1467 t
ORIGIN      
        1 tgtatcactt tattaaccgt ttgcaaatat aacaaattat ttttacatgc atatactgat
       61 attattcgat gtaaagaaag gtgtgaatat tttgagatat tatccattcg atgatagaac
      121 ttacaagcaa cgaaataaaa ataaaatgtt tactctgaac caggcacctc cggcagaaac
      181 cccccaacca cctgtacccc ctcaaccacc agcagttact caacccaagc tggatacagg
      241 caacctgtcg gtcagtgttt ttacagcgtc tggggcactg ccggtacctg atgcattagt
      301 tacagtctat actcatgatg aaagcggtgt agaaaatgaa atagaacgtc atgtaacaga
      361 tgccaatggt caaattcctc tgattacgct gcccgtaatt tatgacccct ttaatcctct
      421 tgaaagcagc gaatattttt tcactaccta taatttaaga gtccaggcaa ttaattacta
      481 tacggtaaat ataattgatc taagaatttt ccctaacact acaacatcct atcagattga
      541 tatgattcct gctgcaatag gccccgtaaa aggtcctgat acaacaattg ttatcccgcc
      601 aagtccggtt gatatatcaa atgattaagg aggtattatt atgagtatag gaattccagg
      661 actaacagag gtggttattc ccgattcaat aactgtccac ttaggcgcgc cggatcagcc
      721 tgctgaaaat attacggttc catttcttga ttacatcaaa aatgtcgcat caagcgaaat
      781 ttatcctacc tggcctgaaa gtgcacttag agctaatatc tatgcgatta cttccatagc
      841 attaaaccgt gtatttacag aatggtacag gtctcaaggt tataattttg acataactag
      901 cgatacaaga tttgaccagg catttgtcaa taacagaggt atttttgaca acgtatcggc
      961 gctggtagat gagatttttg acagctacat agtaaggcag gggcaaatag agccccttta
     1021 tgcccagttt tgcgacggcc gtgtttccca gtgtccggga ctccttcagt ggggtactgt
     1081 tgaattggca aaggaaggtt tgactccata tgaaatgctt cagtattatt atggtgcaaa
     1141 catcaatata caaactgata ctcctgtagc tgcagctttt gaaacatacc ccgggattaa
     1201 ccttaagccg ggggataata atatttatat cttgctgatg cagatttatt taaacaccat
     1261 agctaataac tatcctgcca ttacaagaat accaaatcct acgggtacct tcgatgaaac
     1321 tacacaagca gccgtaagcc agttccaaag aatttttaat ctaccggtaa cgggaattat
     1381 tgataaagca acctggtaca gaataagaag aatttatgta gcagttacaa gtttagcggg
     1441 tttaacttca caaggggtcg tattgagcga aattccccaa tacacgccaa ctcccggccc
     1501 tgaagaagtg gtgcccaggg tccaggctgt ccagtatttt ctcaatgttc tttcagcata
     1561 ttacaatggt attccttccg ttgatattaa cggagtattg gatacccgca ccagaactgc
     1621 cataatggaa tttcagagaa tatttaattt gcccatcaca ggccttgtgg atgaacaaac
     1681 ctggtataca atgtacgatg cagtatcagg tattttaagg acacttcccc cttctgccat
     1741 tgccctgcct gcaattttat ggcccggcat aacatatcaa cttggctcgg aaggacccgg
     1801 cgtttacttt attcagcaat atctgtctta tattgcttct gtactggaag gtatcccacc
     1861 tgctgctcct gatggaatct acggacctga gacggaacgt gcagtcagga cattccaaga
     1921 gtatttcgga attgatgtta caggtgtggt tgacagatac acctggggca gaatagttct
     1981 tatttacaga aatttaaggt ttggaaatac aggcaacata ggtggacttc agtgaaggta
     2041 aatgtcaaaa ccaccggcaa tgccggtggt tagtaatact gtaaaatctc cttttctttt
     2101 tcactatgct gctatacagc caaaattttt caaaaaaatt acagaggcat aagcccaaag
     2161 tagcttaata accccaaata tattccgtaa gcaacgttcc ataggttatc ccttaaaatc
     2221 attgcatcat tataattggt caaatatccc atttcaacca ggacagccgg catgtttgta
     2281 tttctcaata catacaaaga ccggttctcc cttatcccgt tgtctctgag ccttgtattc
     2341 cgcacaattc cattcataat atgctgggcc agccagttag cctgggtatt caactgatat
     2401 atatacattt cagatccgtt aatagcagga ttaacattgg catttgaatg aaggctgata
     2461 aaataattag ccggccactc atttgccatc cggacccttt ccgccaggct ggaggctgta
     2521 tttgttccca atactgtggt ggctataggt cttgataatc tgtattcaaa tctataatca
     2581 ttagccagca ggtttgcaag aagaatacct gcccggtaat ttatttcact ttcatttaac
     2641 ccgttcccta cagctcctgc attgtgaaag cccgtaggat tgtgtccctg atctataaat
     2701 attttaattg ccaaaaaaaa tcacttcctt cattatatac tatcttattt tattcctata
     2761 gtaatgaagt gtgatttttt atttgtattt ccggatatta accttttttt caaatactgt
     2821 tcctgtaaca aaagtgtgtt cccgcacttg acagccatca atggtcagac ttgagtccgg
     2881 ctccgcacat gggaatcaag gcgggagtcc ttccatttaa ttcacagtat ttcatatatg
     2941 ctgcataggt ggccgcagta gtatgctcaa tgtaaatacc ctttgcagca agcattgccc
     3001 tggcttccaa gattctgttt tcaggagcag ttataacttc aatattgtat ttgtaaatgt
     3061 atcctagtat ttcttcgcct ctcattggct ctccaatggc aattccctcc gccatagtgg
     3121 gcacgggtgt taccttgaca ggtctttttt ctttcagtgc cgccgccctt acaaaaggat
     3181 cgcagcattc gctctgcact gcaattatgt gaggcatctt atctattatg ccgctttcaa
     3241 taaactcttc caaggccttt attataccga taaagagagt gccgtttccc aggggtataa
     3301 aaatattttg tggaattctt ttaagctgct catatacttc ataaatatat gttttggtgc
     3361 cttcataaaa gaagggattg aatacatggt tggcataata cttcccttct ttatccgcct
     3421 tggctctgca aacgtcggca cagtggtctc ttgagcccgg aactacatta acctttgcct
     3481 tgtgtgaaag aatcatatcg attttcttgg gtgatgttcc ctcaggtaca aaaatctcac
     3541 agtcaatgcc tgcctttgca cagtatgcag ccacactgtt tcctgcattg ccgctgctgt
     3601 cctggactac tgaatcaaca cctattgcct tgcaatgggc tatcaggact gcagcccctc
     3661 tgtccttaaa agacagtgta ggcatgaaat aatccatctt cagcaaaaca tcttcattta
     3721 actgtatgac aggcgtcaag ccttccccca ggcttatatt ttcccactca ttgccgggaa
     3781 ggggcatgaa tgcccggtaa cgaaaaatac tccagttatc tctgtccaca agtgaaaggt
     3841 caaaacctgg agatttgtaa tccaatttcc atagcccgcc gcagctgcac cttgactttc
     3901 cggttgcagc atccattgtt aagccgcatt tagaacaaat atacttcatc tattttacct
     3961 caaatgatat ataaagtggt aaaactacat ttagaataaa tactttgttt attttttctt
     4021 ctttaaaaac ccgcccagca taccggttat atcatcaact atgctgccgt catcatctgc
     4081 atcaagaagt ttagaaacag aatccataag attattgtcc ttgccttgtc ccaatgcacc
     4141 tgaaagcatt ctggccaatc ctgaagaatc tactttttgt gatgcctttt gctttcctaa
     4201 tgttcccagt aaaagcggag ccagctgggc catcaagtct gtggtttgat ctgctttcat
     4261 tcctgtttgt ttggcaagat ttctctgaat tgatttattc ttgcttccga gtatatggtt
     4321 aagtatcttt gagccgtcct ctttgttcac attcttcaag aaatcttcta tattgtctgt
     4381 ttcatcgtct ttgtggaaat ccaaagcctt cgccagtgat gaagccccct cggctgttga
     4441 ggcgtttttt cccattgcct gcatcaatgc cggcagtccc aatttcatag cctgctgaat
     4501 ctgtgatgaa tcagcccctg ttgacttgct taattgtttc agtacatttt catttccaag
     4561 ctgtgatgac agcatttcca ttaaatccat atcttcctcc ttgcgtgata tagcagataa
     4621 ctaaatcata cgctcctttt ttaaatttat ttttctattt tagtatatac ccataatcac
     4681 attattacaa acaaataatg acaaattaca aaaggc
//
LOCUS       DAOPUT010000104         4688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_18383_length_4688_cov_18.848478, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000104 DAOPUT010000000
VERSION     DAOPUT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18383_length_4688_cov_18.848478"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PKK75_07505"
     CDS             <1..902
                     /locus_tag="PKK75_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNZ83483.1"
                     /translation="FSAPLPAEIQGTTSISIIWKLRLPRSLLAFLVGGAVAVSGSVMQ
                     SVLKNPLASSYTLGVSSGASLGAAVVIVTGFNIPFLGFLTLPATGLIFGMVTVFSAVK
                     FASMMDKNLETSTIILVGMVFSLFVNAILTLIMSIARDHLAQLTFWQMGSFSLKDWSS
                     VIILFPIVLISVIILQYFSKELDIMTFGEDQAFTMGVDVHKIKWVLLGLSAALTGSAI
                     AFVGVIGFIDLIAPHVVRKVFGSSHRIVIPMSMFFGGSFMVICDLIARTIISPSELPV
                     GAITAIIGAPFFAYVYFSGRKGL"
     gene            903..1706
                     /locus_tag="PKK75_07510"
     CDS             903..1706
                     /locus_tag="PKK75_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ83484.1"
                     /translation="MLTLQNVNAGYNGIDVIYNISLSVKEGENLCIIGPNGCGKTTLI
                     KAISGIIPHEGIIKIDGIDINNMKREQVAEKIAVMAQISAVYFSYSIYETVLLGRYLH
                     MKGRTFKEPSLKDKEYTDKCLKAVGLYNIKHKQINTLSGGQLQRVYLARTLVQEPSII
                     LLDEPTNHLDLKHQAELIDFLRDWSKCKGHSVIGVMHDLNLAIKLSDNMLVLDNGNMA
                     AYGNTNKIISSNLLNDVYDMNVTEYMIDSLRQWEAYDSYYNKSKLRKVN"
     gene            1846..2871
                     /locus_tag="PKK75_07515"
     CDS             1846..2871
                     /locus_tag="PKK75_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNZ83485.1"
                     /translation="MKKLLLILITLAAILTACDNINPKPEVPGNDEEVSIEEYYPIKE
                     NTKYSYEGEGNEYASYTVFVDYSSDDRIQTRTNNGGTEVVNVLEVKDDQLTLLYSRGE
                     TYFRQNFLNDEYEGGKILLKGPLKEGSSWDYDENTIASVTDLSKEVVTSLGNYQALEV
                     TLEGEQGKTVNYYAKDVGLVKTISTGEGYEVSSTLSSIENNIPLIQTITLFYPNIDGS
                     LDKIDVQIQFKTNQEPIDVIEEVVKDLSVYEIFSPNTKINELRFDKEENSVHIDLSKD
                     FITEMNAGSSFEALILQSLTNTLGNYYGVQEVYITIDGGPYESGHIIIEEGEPSTVNY
                     DNVNPGE"
     gene            complement(2897..4288)
                     /locus_tag="PKK75_07520"
     CDS             complement(2897..4288)
                     /locus_tag="PKK75_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNZ83486.1"
                     /translation="MKRIFMKNKRLSGLVLGIFLAIIIYFLPLNGLSSQGKICLALTL
                     MTVVFWAFQIVQTGYSSGLYLMLLIIFKAAETEVVLSPWLGSTMYLVIGAYLIASAVK
                     SSGLGERIAYNFILRFVNDYKSVIISIFFLTFILSIIIPHPWPRAFIIMSVISVVIKS
                     AKMPKEDSIKVGFTVFASAVPVSMIFLTGDSLINSLAVQSSKTSVSWLQWLIYMGPPM
                     LIASVITCILILILFKPSKEIKINKEEIKEELDSLGALTNIEKRTAAWLMIAVVLWLT
                     DSVHGIDIGWITLLIAMLMSFPGIGDILGRKQWADVPIHVLIFLNAAMAIGKVGGATG
                     MNSWIAQTVLPSSVPSNLYLLAIVTALISMGIHMLIGSVVAVISIAIPALVIYTESLN
                     ISPLAVTLLVYSSVGIHYILPFHHLDVLVGQGEENGMYGQKEVMRLGIPLTIVVLVVV
                     LFEISYWKIIGLL"
     gene            complement(4530..>4688)
                     /locus_tag="PKK75_07525"
     CDS             complement(4530..>4688)
                     /locus_tag="PKK75_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HNZ83487.1"
                     /translation="FNVIDKKVLLDAQKNPEEYKDLIVRVAGYSDHFNNLEKALQDEI
                     IGRTEQTF"
BASE COUNT         1544 a          841 c          874 g         1429 t
ORIGIN      
        1 tattttctgc accccttcct gctgagatac aaggaaccac ttccatatca ataatatgga
       61 aattaagact tcccaggtct ctcttggcat ttttagtagg cggggctgta gctgtaagcg
      121 gttcagtaat gcagtcggtt ttaaaaaatc cacttgcatc atcctatacc ttgggagtgt
      181 cttcaggtgc ttcattggga gcagcagtag ttatagttac agggttcaat ataccttttt
      241 tgggattttt gactctgcct gccaccggct tgatatttgg catggttaca gtcttttctg
      301 ctgtaaagtt tgcatccatg atggataaaa atttagaaac cagcacaatt attttggtag
      361 gcatggtttt ttcattgttt gtcaatgcca tattgactct cataatgtca attgcaagag
      421 accaccttgc ccagcttact ttttggcaga tgggaagttt ttcattgaag gactggtcaa
      481 gtgtaataat actgtttcct atagtactaa taagtgtaat aatattacag tatttttcaa
      541 aagaattgga tattatgacc tttggagaag accaggcatt caccatggga gtggatgttc
      601 acaaaatcaa gtgggtgctc cttggtcttt cagcggcact tacaggcagc gctattgcat
      661 ttgtaggagt tatagggttc atagacttaa ttgctcctca tgtggttaga aaagtattcg
      721 ggtccagcca cagaatagta attcctatgt caatgttttt cggcggctcg ttcatggtta
      781 tctgcgacct aatagccaga acaatcatat caccttccga attgccggta ggagcaatta
      841 ccgccataat aggcgcgcca ttttttgcct atgtatactt ttcaggaaga aaggggttat
      901 aaatgttaac tttacaaaat gttaatgcag gttataacgg aatagatgta atatataata
      961 tttccttaag tgtaaaggag ggtgaaaatc tttgcattat aggtcccaac ggctgtggaa
     1021 agacgaccct tattaaagca atttcaggaa ttatccctca tgaaggtata attaagatag
     1081 acggaattga tataaataat atgaagagag agcaggttgc agaaaaaatt gcagttatgg
     1141 ctcaaatttc agctgtttat ttttcataca gcatttacga aacagttctt ctgggaagat
     1201 atcttcatat gaagggcagg acatttaagg aaccgtccct taaggacaaa gaatatacgg
     1261 ataagtgttt aaaagctgta ggcctttata atataaagca caagcagata aacaccttgt
     1321 cgggaggcca gcttcaaagg gtttatcttg ccagaacctt ggttcaggag ccaagcataa
     1381 tacttcttga tgaacctaca aaccatttgg atttaaaaca tcaggcagaa ttaatcgact
     1441 ttttgaggga ttggtcgaag tgtaaaggcc attcagtaat aggtgtaatg catgatttga
     1501 atttagcaat taaattgtct gacaatatgc ttgtactaga caacggaaat atggctgctt
     1561 acggcaacac aaataaaata atttcatcca atcttctaaa tgatgtatat gatatgaatg
     1621 ttacagagta catgattgat tccttaaggc agtgggaggc atatgattcc tattataata
     1681 aaagcaaatt acggaaggta aattaatggg agctttcaga ataaaacgca tatacttagc
     1741 cggcattctg tgataacgaa acagccggct atttttttgt ataagttatt aaaagcattt
     1801 gaattgggta taataaacta accatacttt tgaaaaggag aaattatgaa aaaattatta
     1861 ttgattttga taacgctggc tgcaatactt acagcatgtg ataatataaa tcctaagcct
     1921 gaagtaccgg gtaacgatga ggaagtttca atagaagaat attatcctat taaggaaaat
     1981 acgaaatatt catatgaagg tgaaggaaat gaatatgctt cttatacagt attcgtggat
     2041 tattcttcag atgacaggat tcagacaaga actaacaacg gggggacaga agtagtaaat
     2101 gttctcgaag ttaaggatga tcagcttacc ttgttatact caagaggtga aacatatttt
     2161 agacaaaact ttttaaatga tgaatatgaa ggcggaaaaa tactacttaa aggcccctta
     2221 aaggaaggaa gttcatggga ctacgatgaa aacacaatag catcagttac cgacttgtcc
     2281 aaagaagtgg tcaccagcct tggcaactat caggctcttg aagttacact ggaaggagaa
     2341 cagggaaaaa ccgtaaatta ttatgcaaag gatgtgggat tggttaaaac aataagcacc
     2401 ggtgagggtt atgaagtatc atcaaccttg tcgtcaatag aaaacaatat acccctgata
     2461 caaacaataa ctttatttta tccgaatatt gacggttccc ttgataaaat agatgttcaa
     2521 attcagttca aaaccaacca ggaacctatt gatgttattg aagaagtagt caaagacctg
     2581 tcagtttatg agattttcag ccccaatacc aaaataaatg aactccgctt tgataaagaa
     2641 gaaaacagcg ttcatattga tttatcgaaa gattttataa cagaaatgaa tgcgggatca
     2701 agctttgaag cacttatact tcaaagtctg acaaacacat taggcaacta ctacggtgta
     2761 caggaagttt acataacaat tgacggaggc ccttatgaat ccggacacat aataattgaa
     2821 gaaggagagc cctcaacagt aaactatgac aatgtaaatc cgggagaata gtttgactgt
     2881 cattgaatta ttactgctat aaaagtccga ttatcttcca gtatgaaatt tcaaataaaa
     2941 caacaaccag gacaactata gttaatggaa tacccagacg cattacttct ttttgtccat
     3001 acatgccgtt ttcctcacct tgacctacca aaacatctaa atgatgaaat ggtaaaatat
     3061 agtgtattcc aactgatgaa tatactaata atgttacagc caacggactt atatttaaag
     3121 attccgtata tattaccagt gcaggtattg ctatactgat aactgctaca acactgccta
     3181 taagcatatg aatacccatg gatattaatg ctgttactat tgccaaaaga taaagatttg
     3241 aaggaactga tgaaggaagg acagtctgag caatccatga attcataccg gtggcaccgc
     3301 ccaccttgcc tattgccatg gctgcattca agaatattaa aacatgtatt ggaacatctg
     3361 cccattgctt tcgtcccagt atatctccta ttcccggaaa actcattaac attgcaataa
     3421 gcagtgttat ccagcctata tcaattccat gtactgaatc tgtcagccat agcactactg
     3481 caatcataag ccatgctgct gttctttttt ctatatttgt aagagcacct aaagaatcaa
     3541 gttcttcttt tatttcttct ttatttattt tgatttcctt tgatggttta aataagataa
     3601 gtattaatat gcatgttatt acactggcaa tcagcatcgg aggtcccatg taaataagcc
     3661 attgcagcca gcttactgag gtttttgaag attgtaccgc taaagaattt atcaaactgt
     3721 cgcctgttaa aaatatcatg gaaacaggaa cagcagaagc aaaaacagtg aatccaacct
     3781 ttattgaatc ttcctttggc attttggcgc ttttaatcac aacacttatt accgacatta
     3841 ttataaaagc ccgcggccag gggtgaggta ttataatact taaaataaat gtcaagaaga
     3901 atattgatat aattacgctt ttataatcat taacgaatct caatataaaa ttatatgcta
     3961 ttctttcccc caatcctgaa ctttttactg ctgaagcaat caaatatgct ccaataacca
     4021 ggtacatggt actgccaagc catggagaga gtacaacttc agtttccgct gctttgaaga
     4081 ttatcagcag cattagataa agccctgatg aatacccggt ttgtacaatt tgaaatgccc
     4141 aaaaaactac agtcatgagg gttaaagcta aacatatttt cccttgtgat gacaagccgt
     4201 ttagtggaag aaagtaaatt attattgcta aaaaaatacc tagaactaaa ccggacagcc
     4261 ttttattttt cataaaaatc ctcttcataa tataataata tacccctttg tttataccga
     4321 taatgggctc gtttcattct ctttataatt ttttttaaaa tgaccaaagg ctaagcctat
     4381 ggtcgttaaa ctgctgtgct aagataacac cctgcagtct cattcaccaa tgtatttgct
     4441 ccctttggtc gcatacattg ggaattcgtt gcgaatgacc taccgctatt ttccagaaaa
     4501 aaactctgaa aaatagggtt agggcatctt taaaaggtct gctctgttct tccgattatt
     4561 tcatcctgca gtgctttctc caggttgttg aaatggtcgg aataacctgc caccctgact
     4621 attaaatcct tgtattcctc cggatttttt tgtgcgtcca aaagaacctt tttatcaatt
     4681 acattaaa
//
LOCUS       DAOPUT010000105         4666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_18494_length_4666_cov_17.736066, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000105 DAOPUT010000000
VERSION     DAOPUT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18494_length_4666_cov_17.736066"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..964)
                     /gene="ftcD"
                     /locus_tag="PKK75_07530"
     CDS             complement(74..964)
                     /gene="ftcD"
                     /locus_tag="PKK75_07530"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNZ83488.1"
                     /translation="MDKLIECVPNFSEGRNLEKIDKLLDVFRGRKGVKLLDYSSDEDH
                     NRTVVTIVGEPEEVKKAMVEAIGRAVDLIDLNFHEGQHPRMGAVDVVPFIPIRNVTVE
                     EADKLAKDVAKEASEKYGLPFFLYEKSASAPHRENLANVRKGQFEGMKEKMQDELWKP
                     DFGPDTIHPTAGVTAIGARMPLVAFNVNLSTSDIEIANRIAKQARYISGGFRYVKAIG
                     VELHDRGIVQVSMNLTDYTKTSLYRVFETIKMEAARYGVNVVGSEIIGLIPMQALVDT
                     ADYYLRLENFKFDQVLETRL"
     gene            complement(957..>2045)
                     /locus_tag="PKK75_07535"
     CDS             complement(957..>2045)
                     /locus_tag="PKK75_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNZ83489.1"
                     /translation="AYHGNIVDLLEYAVDNNIHVDLLSDQTSCHVPYDGGYCPKGLTF
                     EERTRLLATDREEFARMVDKSLADHFKAVKTLVDRGTYFFDYGNAFMKAVFDSGVKEI
                     SKNGVDTSEGFIFPSYVEDIMGPMLFDYGYGPFRWVCLSGKPEDLHKTDQAAMSVIDP
                     YRRGQDRDNYIWIRDAEKNKLVVGTQARILYQDALGRRDIALKFNDMVRKGEIGPVML
                     GRDHHDTGGTDSPFRETSNIYDGSNVTADMAVHCYAGNAARGMSLVALHNGGGVGISK
                     AINGGFGLVLDGSERVDKIINASIPWDTMVGVSRRSWARNEHSIETTIEYNKKFRGQD
                     HITIPFLADDELIEKTLKNYTVTGEQNG"
     assembly_gap    2046..2145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2146..2855)
                     /locus_tag="PKK75_07540"
     CDS             complement(<2146..2855)
                     /locus_tag="PKK75_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNZ83490.1"
                     /translation="MTDNISISNAMKIKLSDELPEYPVLKENVRRAPKREVTLNEREI
                     KLALKNALRYIPESLHEKIAPEFLDELMIHGRIYGYRFIPKERIYGRPIHEYKGKCIE
                     GKAFQVMIDNNLNHEVALYPYELVTYGETGQVCQNWMQYQLIMKYLEELTDEQTLAVM
                     SGHPLGLFKSHKSSPRVIISNGLMIGMFDNPQDWTKATAMGCSSYGQMTAGGWMYIGP
                     QGIVHGTFNTLINAGRKEL"
     gene            complement(3029..>3423)
                     /locus_tag="PKK75_07545"
     CDS             complement(3029..>3423)
                     /locus_tag="PKK75_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNZ83491.1"
                     /translation="EMNKKWCLESGCKDDEGLSDENCATKYFFKYFRELELEGGLIRV
                     NNEVVAYTIGEVLNSDTYVIHIEKAFKDVQGAYTMINQQYAAWVKEKYPHLIYMNREE
                     DMGLEGLRKAKLSYYPDKMADKYRAVLR"
     assembly_gap    3424..3523
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3524..3710)
                     /locus_tag="PKK75_07550"
     CDS             complement(<3524..3710)
                     /locus_tag="PKK75_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83492.1"
                     /translation="MINFKKIELEDKKWIIPILKGSVISGCHQNFGNLFAWRDINDTK
                     VAKVNDFLVATQTIGNIK"
     gene            complement(3951..4529)
                     /locus_tag="PKK75_07555"
     CDS             complement(3951..4529)
                     /locus_tag="PKK75_07555"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNZ83493.1"
                     /translation="MDFAEYSDIFLNYVETWGPVAGILLPVVEAFIPILPLVGFVIIN
                     VAAFGFFFGYLYSWIGNCLGSFLLFLLLRKAGARRINEKISRSKYRHTLEKVKKKQLN
                     VLFILYCFPFAPSFLLSGAAALTNMSTEMFLVILLPSKFVMLLSLAFIGQNVSSFFEN
                     PAKSIFFIIFIVLLNLLVKYGFEKYEHYHRKK"
BASE COUNT         1184 a         1001 c          781 g         1500 t
ORIGIN      
        1 tcccccttca agtacgggga cattccttct accctcaaag actatcccga aagagaaggt
       61 gtgtccccct tccttatagc cgggtttcca acacttggtc gaatttaaag ttttccaggc
      121 ggagatagta gtctgctgta tccaccaatg cctgcattgg aatcaaacca ataatttcgc
      181 ttccaacaac attaactcca taccttgccg cttccatttt tatagtttca aatacacggt
      241 atagggatgt ctttgtgtag tctgttaaat tcattgacac ctgcacaatt cctctgtcat
      301 gcaattcaac tcctatagct tttacatacc taaatcctcc gcttatatat cttgcctgct
      361 ttgcaattct atttgcaatt tcaatatcag aggttgataa gttcacattg aaggcaacca
      421 agggcatcct tgcgcctatt gcagtaaccc ctgctgttgg atggattgtg tcaggaccga
      481 agtccggctt ccataattca tcctgcattt tttccttcat tccttcaaac tgtccttttc
      541 tcacattggc aagattttct ctatgagggg cggatgcaga cttttcataa aggaaaaaag
      601 ggaggccata tttttctgaa gcttcctttg ccacatcctt ggcaagcttg tcagcttctt
      661 ccacagtaac atttcttata ggtatgaaag gaaccacatc cacggctccc atgcggggat
      721 gctgtccttc atggaaattc aggtcaataa ggtccactgc ccttccgata gcttcgacca
      781 tagctttttt aacctcttca ggctcgccca caattgttac aacagtgcgg ttgtggtcct
      841 catcgcttga atagtccaaa agcttgaccc cttttcttcc cctgaaaaca tccaggagct
      901 tgtctatttt ttccaggttc ctgccttcac tgaagttagg aacgcattcg attagtttat
      961 ccattttgtt ctccggtaac agtgtaattt tttaaggttt tttcaattaa ttcatcatct
     1021 gctaaaaatg gtatggtaat gtggtcttgg cctcggaatt ttttgttgta ttcaattgtt
     1081 gtttcaattg agtgttcatt tcttgcccag cttcttcttg acactccaac catggtatcc
     1141 cagggaatgg aagcatttat tattttgtcg actctttcgc ttccatccag aacaagcccg
     1201 aagcctccgt tgattgcctt gctgatgcct actcctcccc cgttgtgaag ggcaaccagg
     1261 gacattcctc ttgccgcatt ccctgcatag caatggacag ccatatcagc ggtaacattg
     1321 ctgccgtcgt aaatatttga tgtttccctg aatggagaat ccgtgccgcc tgtgtcatga
     1381 tggtcccttc caagcattac aggaccgatt tctccttttc ttaccatgtc gttgaattta
     1441 agggctatat cccgtctgcc aagcgcatcc tggtataaaa ttctcgcctg ggttccaaca
     1501 acaagtttat ttttttccgc atcccttatc catatataat tgtccctgtc ctgacctctc
     1561 ctgtacggat ctattactga cattgccgcc tggtctgtct tatgcagatc ttcaggcttt
     1621 ccgcttaggc aaacccatct gaatggtccg tatccgtaat caaaaagcat tggtcccata
     1681 atatcctcca catatgaggg gaatatgaag ccttcgctgg tgtctactcc atttttggaa
     1741 atttccttta ctcccgaatc aaaaacagcc ttcatgaatg catttccgta gtcaaagaaa
     1801 tatgtgcctc tgtcaaccag tgtttttacg gccttgaagt ggtcagccaa ggacttatcc
     1861 accatccggg caaattcttc cctgtcagta gccagcaatc tggttctttc ctcaaaggtc
     1921 agtcctttgg gacaataacc cccgtcatag ggcacatggc aggaagtctg gtcagacaac
     1981 aaatccacat gtatgttgtt atctactgca tattccagca ggtctacaat attgccgtgg
     2041 tatgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagctc ttttctgcct
     2161 gcattgataa gggtgttgaa tgttccgtgg acaattccct gaggtcctat gtacatccag
     2221 ccgcctgcag tcatctgccc gtagcttgaa catcccatag ccgtggcttt ggtccagtcc
     2281 tgagggttgt caaacatacc tatcattaaa ccgtttgata ttataactct ggggctgctt
     2341 ttatgagatt taaaaagtcc cagggggtgg cctgacataa cagccagtgt ctgttcatct
     2401 gtcagctctt caagatattt cataattaac tgatactgca tccagttctg gcaaacctgg
     2461 cctgtttccc cgtaggtaac aagctcatag gggtaaaggg caacctcgtg gtttaagttg
     2521 ttgtctatca ttacctggaa tgcctttcct tcaatgcatt ttcccttgta ctcatgaatg
     2581 ggtctgccgt atattctttc tttgggtatg aaacggtagc cgtaaattct gccatgaatc
     2641 ataagctcgt ccaggaattc gggggctatt ttttcatgga gactttccgg aatatacctt
     2701 agagcatttt tcagtgccag cttgatttcc ctttcattta aggttacttc tcttttggga
     2761 gcacgtctta cattttcctt taagacagga tactcaggca actcatcact cagcttgatt
     2821 ttcattgcat ttgatatgct tatattatcg gtcatctttt acacctccaa aattatttta
     2881 aagattatat ataacttaag attacttaaa gctttttcgg gtaaactatg aatgtaagtt
     2941 aatagtgaat actaattaac ttccctctcc cccttgtttt tttataaata ttgtgttatg
     3001 ccttacatgt gtgaggcata attctttttt atctgaggac tgcacggtat ttatccgcca
     3061 tcttgtccgg ataatatgaa agctttgcct ttcttaagcc ttctaggccc atgtcttctt
     3121 ctctgttcat ataaatcaaa tgaggatact tttccttgac ccatgctgca tactgttgat
     3181 ttatcatggt atatgcccct tgtacatcct taaatgcctt ttcgatatgt attacatatg
     3241 tatctgaatt tagaacttca cctatggtgt aagccacaac ttcgttgtta actcttatta
     3301 aaccgccttc aagttcaagc tccctgaaat atttaaagaa atactttgtt gcacagttct
     3361 catccgacag gccctcgtcg tccttgcacc ctgattcaag gcaccatttt ttgttcattt
     3421 caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttttat attgccgatt
     3541 gtttgtgtag caaccaaaaa atcattgact tttgcaacct ttgtatcgtt aatatccctc
     3601 caggcgaata aatttccgaa attctgatga catcctgaaa tcaccgagcc tttcaatatt
     3661 ggaattatcc atttcttgtc ttccaattca atttttttaa agtttatcat taatttgatt
     3721 ttctccttgc tgtcgtccat tttattatta tgttttatac agtagtttta ttatacaata
     3781 tgttttagaa aaaaacactg aaatatatta aatcaagaac atgattatta atatttttta
     3841 tttgagtttt caatgacagg gtgaccgggc tgttctgtac atcataaaca taggaacaga
     3901 agtatctgtt ttctgtaaca atactctgat aaggaaacag ggtacagtgc ttactttttt
     3961 ctgtgataat gctcgtattt ttcaaagccg tacttgacta aaagattcaa aagaacaata
     4021 aatattatga agaatatgga ctttgcagga ttttcaaaga atgagctcac attttgacct
     4081 ataaaagcca gggataaaag cattacaaat tttgatggca ggaggattac aagaaacatt
     4141 tcagtactca tatttgtcaa ggctgcagca ccgcttaata aaaatgaggg tgcgaaggga
     4201 aagcagtaca atataaatag aacgtttaat tgtttcttct ttaccttttc aagtgtgtgg
     4261 cgatacttgc tcctgcttat cttttcattg attcttcttg cccccgcctt tctcagaagc
     4321 aagaacaaaa gaaatgaccc taagcagttt ccaatccagg aatacaggta gccaaagaaa
     4381 aatccgaatg cggctacatt tattattaca aaacctacca atggaagaat gggaataaat
     4441 gcttctacca caggaagaag tattcctgcg acaggtcccc atgtttcaac ataatttaaa
     4501 aaaatatcag aatattctgc aaaatccatt atcaatttcc tttatttaaa gtgtacttat
     4561 tatacccagc cttatattaa catacaaatc caaacaaaat attaattgtg taaaatatag
     4621 gaatctctgt taatgttatc attatgcttg attattttca ataaaa
//
LOCUS       DAOPUT010000106         4550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_19117_length_4550_cov_19.077197, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000106 DAOPUT010000000
VERSION     DAOPUT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19117_length_4550_cov_19.077197"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKK75_07560"
     CDS             <1..281
                     /locus_tag="PKK75_07560"
                     /inference="COORDINATES: protein motif:HMM:NF021125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HNZ83494.1"
                     /translation="AISKSCLIGLDEVCGFTQDNIDDNVIIDVTIGVTRPDEVNMDEV
                     IKCFPVGKVNVRAVFGGLKTEGLYFTKFGDKDDSIEIAVASVSVKIKK"
     gene            281..1282
                     /locus_tag="PKK75_07565"
     CDS             281..1282
                     /locus_tag="PKK75_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNZ83495.1"
                     /translation="MGGSILKITNEQLKEMYFTMLKIRKFELSASDLFAQGKIPGFVH
                     LYIGEEAVATGACANLREDDYITSTHRGHGHIIAKGGELKYMMAELFGKEDGYCKGKG
                     GSMHIADATRGILGANGIVGAGHNIAVGSGLAIQHKKTDQVCVCFFGDASTNQSTFHE
                     ALNLASVWKLPVIFICENNGYGISVSQARHQAITDVSVRASAYNMPGITIDGNDLFAV
                     YEAVGEAAARARKGQGPTLIECKTYRWRGHFEGDPVVYRPEGELEAWLKKDPIPRVEK
                     YFINNGVMTEEEIKKLNDDVEKMVEEAIVFAEKSPVPSLESALKDVYSDIVEEGRVR"
     gene            1279..2253
                     /locus_tag="PKK75_07570"
     CDS             1279..2253
                     /locus_tag="PKK75_07570"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNZ83496.1"
                     /translation="MKKMTYAEGIREAMRLKMQEDPDVILFGEDVGKFGGCFGVSGDL
                     WHEFGDERVRDTPISEGVIVGAAVGSAACGLKPIAELMFIDFMTVGMDQLVNQAAKMR
                     YMFGGEINLPMVLRVPAGAGIGAAAQHSQSLEAWATHVPGLKVVYPSNAQDAYGLLLT
                     SIDDQNPVVFVENKVLYGMKNDVDENELKPIPLGKARIAREGTDVSIITYGRQVYDAL
                     QAAEELAKDGINAEVIDLRTLVPIDKETIFNSVEKTHKVVVVSEECKRGAYTADIAAM
                     IAEERFYECDAPIMRIGALNTPIPFATGLEKYVVPNSSDIVTAVKSLF"
     gene            2318..3316
                     /locus_tag="PKK75_07575"
     CDS             2318..3316
                     /locus_tag="PKK75_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNZ83497.1"
                     /translation="MASIGIIANPASGKDIRRLVSHATVIDNNEKINIVERIVLGAQA
                     LGVEKVFVMPDSYNMGYRVEDKLNSCNELKCQINVINMMRFDGMEDTVKAADYMEKNN
                     EIKCIVTLGGDGTNRAAAKKIINTPIITISTGTNNVYPTMLEGTIAGMAAAVVASGSF
                     DETGYISRDKRIEIYRGGDLIDIALIDAVISKDVFIGSKAIWNMDSIEKIVVTRAHPA
                     SIGFSSLVGCKKIIYPEDDFGALVDINSGSKMIKAPIAAGVVESIHVSEPVILGLDDE
                     YEFTAEDRGIIALDGEREIEFSKDQKFIFKVRRRGPFHADVVKALETAQLNGFFTL"
     gene            3337..>4550
                     /locus_tag="PKK75_07580"
     CDS             3337..>4550
                     /locus_tag="PKK75_07580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNZ83498.1"
                     /translation="MAEFIVMPKMGLTMTKGLLTNWRKKEGDTVNKGDILFDVETDKL
                     TNKIEAKTSGVLRKILVDKETVNILVPVAIIGTADEDISELLKEAKAEGSEKEEAQKK
                     EAPQQEAPPAPVRKPGERVKISPRAKKTAEDLNVDYNLVPGTGPGGAITEDDIRKFAE
                     RPKAKVSPTAAVLAEQLGVDTAVIEKDTRIMKADVVKYKLDENLAKYASPQEYRQPMT
                     NMRKIIAERMLVSVQTSPSVSFNIKVDTTGMKQLRDDLKDTFKVSYTDILVKIVSQVL
                     LQYPLLNSSLDSEEILTRNYVNMGVAVALPEGLLVPVVRYANAKGLKDISGEIKDLGE
                     RAKTNNLLPDELSGGTFTISNLGMYGIESFTPIINQPEVAILGVNAINKEPVVVDDQV
                     LVKPMMNLSLTA"
BASE COUNT         1537 a          754 c         1121 g         1138 t
ORIGIN      
        1 atgccatatc aaaaagctgc cttataggtc ttgatgaagt ttgtggattt acccaggata
       61 acatagatga taacgttatc atagatgtta caataggggt aactcgtcct gatgaagtaa
      121 atatggatga ggttattaag tgctttcccg tagggaaggt aaatgtgcga gcagtttttg
      181 gaggtttaaa aactgaaggt ctttatttta caaagtttgg tgataaggat gactcaatcg
      241 aaattgctgt tgccagtgta tctgtaaaaa ttaaaaaata atgggaggta gtattttgaa
      301 gatcactaac gaacaattga aggaaatgta ttttacaatg ctgaaaattc gaaaatttga
      361 attaagtgct tcggatttat ttgctcaagg aaaaattccg ggctttgtac acctgtatat
      421 tggtgaagaa gctgttgcca caggggcttg cgcaaacttg agggaagatg actacataac
      481 ctccacccat aggggtcacg gccacataat agccaagggc ggagaattaa agtacatgat
      541 ggctgaactt ttcgggaaag aagacgggta ttgcaaagga aaaggcggct caatgcatat
      601 tgcagacgcc accagaggaa ttttgggagc aaacggcata gttggcgccg gtcataacat
      661 tgcagtgggt tctggcctgg caattcaaca caagaaaaca gaccaggtat gcgtatgctt
      721 cttcggagat gcatcgacta accagtccac atttcatgaa gctttgaact tggccagcgt
      781 ttggaaactg ccggtaattt ttatatgtga aaacaacggt tacggaattt cagtcagtca
      841 ggcaagacat caggctataa cggatgtttc cgtcagagca tccgcatata atatgcccgg
      901 cattacaata gatggaaatg atttgtttgc agtttatgag gctgttggtg aagcagctgc
      961 aagagcaaga aaggggcagg ggccgacact tattgagtgt aaaacataca gatggagagg
     1021 acattttgaa ggagacccgg ttgtatacag acctgaggga gaattggaag catggcttaa
     1081 gaaagatcct attccaagag tagaaaaata ctttataaat aacggtgtga tgacagaaga
     1141 ggaaataaag aaattgaatg atgatgttga aaagatggtt gaagaagcaa tagtttttgc
     1201 tgaaaagagc cctgttccat cattagaatc agctttaaaa gatgtgtatt ctgatatagt
     1261 tgaggaggga agagtaagat gaaaaagatg acatatgcag aaggaatcag ggaagcaatg
     1321 cggttaaaaa tgcaggaaga tcccgatgtt atactgtttg gtgaagatgt tggaaaattc
     1381 ggtggatgct ttggcgtcag cggagattta tggcatgaat tcggtgacga aagagtaaga
     1441 gacactccta tatcagaagg tgtaatagtg ggagctgctg tagggtctgc agcatgcggc
     1501 ttaaaaccca tagctgaact catgtttatt gacttcatga ctgtcggcat ggaccaattg
     1561 gttaaccagg ccgctaaaat gagatacatg ttcggcggtg aaataaatct tccgatggtt
     1621 ttaagagttc ctgcaggcgc aggcattggt gccgctgcac agcattcaca gtctttggaa
     1681 gcatgggcaa cacatgttcc gggacttaag gttgtgtatc cctcaaatgc ccaggatgca
     1741 tacggcctgt tattaacatc aatcgatgac caaaatcctg tagtatttgt tgaaaacaaa
     1801 gtattgtatg gaatgaaaaa cgatgttgac gaaaatgaat taaaaccaat tcctttgggt
     1861 aaagcaagaa ttgccaggga aggtaccgat gtttccataa tcacatacgg acgccaggtt
     1921 tacgatgccc ttcaggcagc tgaagaactt gcaaaggatg gaataaatgc agaggttatc
     1981 gatttaagga cacttgttcc tatcgacaag gaaaccatat ttaattcagt ggagaaaacc
     2041 cataaggttg ttgtggtaag cgaagagtgc aagagaggag catatacggc agatattgca
     2101 gcaatgatag cagaagaacg cttctacgaa tgcgatgctc ctataatgag aataggtgcc
     2161 cttaatactc caataccatt tgccaccggc cttgaaaaat atgtggtgcc aaacagcagc
     2221 gacatagtaa ctgctgtaaa atcattattc tagtaactaa ggatacggat ggggctgctg
     2281 catcgggcgg cagccccggt tttgaggagg gcgtaaaatg gcttcaattg gaattattgc
     2341 aaatcccgct tccggaaagg acatcagaag actggtatca catgcaactg taatcgacaa
     2401 caacgaaaaa attaatatag tggagagaat tgtgcttgga gcacaggctt taggggttga
     2461 aaaagttttt gtaatgcctg actcatacaa tatgggatac agggttgaag acaagctgaa
     2521 cagctgcaat gagcttaaat gtcaaataaa tgtaatcaat atgatgcggt ttgacggtat
     2581 ggaggataca gtaaaggctg ctgattacat ggaaaaaaac aatgagataa aatgcatagt
     2641 tacacttgga ggagacggta caaacagagc tgctgcaaag aaaattataa acactccgat
     2701 aattaccatt tctacaggaa ccaacaatgt ttatcctaca atgcttgaag gaaccatcgc
     2761 cggtatggct gcggcggtag ttgcttcagg cagttttgat gagaccgggt atataagcag
     2821 ggacaagagg atagaaatat acagaggcgg agaccttata gatattgcac ttatagatgc
     2881 tgtaatatca aaggatgtat ttatcggctc aaaggcaata tggaatatgg attcaataga
     2941 aaaaatagtt gtgaccagag ctcaccctgc atcaattggt ttttcatcat tagtcgggtg
     3001 taaaaaaata atttatcctg aagatgattt tggtgccttg gttgatatta actcaggcag
     3061 caaaatgatt aaagcaccta ttgctgcagg ggtggtggaa tccatacatg taagcgaacc
     3121 cgtcatttta ggattagatg atgagtatga atttaccgct gaagacagag gcatcatagc
     3181 tttggacggt gaacgggaaa ttgaattttc aaaggaccag aaatttattt ttaaagtcag
     3241 aagaaggggt ccttttcacg cagatgttgt aaaagcattg gaaaccgctc aattaaacgg
     3301 attttttaca ttataggtta aaattaagga ggtagtatgg ctgaatttat agtaatgccg
     3361 aaaatgggac tcaccatgac aaaaggactg ttgacaaatt ggagaaaaaa agaaggggat
     3421 acagtaaata aaggtgatat cctgtttgat gtggaaacag ataaattaac taataaaatt
     3481 gaagcaaaga catccggggt tttaagaaaa atactggttg acaaggaaac cgtaaatatt
     3541 cttgtgcctg ttgcaattat aggcactgct gatgaagata tatcagaatt gttgaaagag
     3601 gcaaaagcag aggggtcaga aaaagaagaa gctcaaaaga aagaagctcc tcaacaggaa
     3661 gcaccgcctg caccggtgag aaagccagga gaaagggtaa aaatttcccc gagggcaaag
     3721 aaaacagctg aggacctgaa tgtggactat aatctggtac cgggaacagg accgggggga
     3781 gcaataacag aggatgatat aagaaaattt gccgagagac caaaagccaa agtatctcct
     3841 acagcggctg ttcttgctga acagttggga gtggacacag ccgttataga aaaagacaca
     3901 aggataatga aggcagatgt ggttaaatac aaactggatg aaaacctggc aaaatatgca
     3961 agcccccagg aataccgcca gcctatgacc aacatgagaa agattatcgc ggaaagaatg
     4021 cttgtaagtg ttcaaacatc cccctctgta tcatttaaca taaaggtgga caccaccggc
     4081 atgaagcagc ttagagatga tttgaaggat acattcaagg tatcctatac agatattctt
     4141 gtaaaaattg tatcccaggt actgcttcaa tatcccctcc tgaactctag tcttgacagc
     4201 gaagaaatat taaccagaaa ttatgttaac atgggagttg ccgtggcact tcctgaaggg
     4261 cttctggttc ctgtagtaag gtatgccaat gcaaaaggct tgaaggatat atccggagaa
     4321 atcaaggacc ttggcgaaag agcaaaaacc aataatttac tgccggatga actaagcgga
     4381 ggaacattta ccataagcaa tttaggaatg tatggaatcg aatcattcac ccccatcatt
     4441 aatcagccgg aagttgcaat tttaggagta aatgcaataa ataaagagcc tgtggttgtt
     4501 gatgatcaag ttcttgtaaa gccaatgatg aatttaagcc ttacggcaga
//
LOCUS       DAOPUT010000107         4535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_19198_length_4535_cov_19.154464, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000107 DAOPUT010000000
VERSION     DAOPUT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19198_length_4535_cov_19.154464"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1112)
                     /locus_tag="PKK75_07585"
     CDS             complement(81..1112)
                     /locus_tag="PKK75_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNZ83499.1"
                     /translation="MNYNELLDRAREKMAPNCRVCKECNGLACKGEIPGTGGKGNGNA
                     FKICLDFLTSVKINMDTIYKNEGQDTSVSLFGRNFKYPFFAAPIGGMSLNYNGSITEA
                     EYIEAIVKGTLKAGNAGFTGDGPADNLFLDSLPIIKEADGRAVPTLKPWKNDKVLEKI
                     KMIEEVNAMAFAMDIDSAGLINLALAGKPVSAKTVEELKELTESTKIPFIVKGVMTAK
                     GAVKAYEAGAYGIVVSSHGGRVLADAPATCAMLPEIKEAVGDKIKIFVDGGIRSGADV
                     FKALALGADAVLIGRPYAIAAFGGGAEGVELYTEKIGAELRETMIMTGCKNISDITRD
                     KIKMPCETP"
     gene            complement(1109..3766)
                     /locus_tag="PKK75_07590"
     CDS             complement(1109..3766)
                     /locus_tag="PKK75_07590"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNZ83500.1"
                     /translation="MSTNIPSKYNPKEFEDKIYKIWMDENCFRAEINKDKKPYTIVMP
                     PPNITGQLHMGHALDQTLQDVLIRWKRMMGYEALWLPGSDHASIATEVKVVERLRKEE
                     GKTKEDIGREEFLNRAWKWKEEFGGMIVDQVKKLGNSCDWSRERFTMDEGCNEAVTEF
                     FIRLYERGLIYKGNRIINWCPDCHTTLSDAEVEHEDTAGKYYYVKYPFADNKDEFFII
                     ATTRPETIFGDVAIAAHPDDERYKHLIGRKVIVPLVGREIPIIADEYPDRSKGTGALK
                     ITPAHDPNDFEIGLRHNLDQLSCMNEDGTMNEIAGKYEGMDRYDCREAFVRDLDDSGF
                     ILKIENTEHNVGTCYRCHNDIEPRISDQWFVKMKPLAEPALKVVEDKEMQLVPERFTK
                     IYNHWLENIRDWCISRQLWWGHRIPAYYCQDCNEIMVQKYSPEKCSKCGSTNITQDED
                     VLDTWFSSGLWPFSTLGWPHETDDLKYFYPTDVLVTGYDIIFFWVIRMVFSALEVTGK
                     SPFKYTFIHGLVRDAEGRKMSKSLGNGIDPLDVIDNYGADALRFMLMTGISPGNDTRF
                     NYDRLESSRNFANKLWNASRFVLMNLDGALTDKETAKKNYKTEDKWIISRANKAAKEA
                     TDYLEKFELGMAAQRVYDFIWNEYCDWYIEIVKPRLYGADGDDKDAARYVLIKVLKDM
                     LKLLHPFMPFITEEIWSYLPDTSSRLVRADWPEYSKEEIFEEAEEALKFIMEAVRSIR
                     NIRAEMNVLPSRKARAIFIPSNEEAKQFIASGAKYFSTLANITEIKLVNNKKDIGEDT
                     ASSVIDGTEIYLPLADLIDYEKEIERLEKEKARLEDELKRVKGKLNNEKFISKAPESV
                     VNEEKEKLQKYELMMEKVLERLEQMGRRI"
     misc_binding    complement(3998..4203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     regulatory      4367..>4535
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1175 a         1039 c          729 g         1592 t
ORIGIN      
        1 cgaaagaaac tgataagttg tctttcgctt tttagttact gcagtcccca aatcatgtct
       61 ctagactctg aaaaataggg ttagggcgtc tcgcagggca tcttaatttt atctcttgtt
      121 atgtcactaa tatttttgca gcctgtcatt atcattgttt ctcttaattc agctccgatt
      181 ttttcggtat acaattccac accttccgca ccgccgccaa aagcagcaat ggcgtagggt
      241 ctgcctatca acactgcatc tgctcccaat gctaaagcct tgaacacatc agctcctgaa
      301 cggattcctc cgtcaacgaa aatcttaatt ttatctccca ctgcttcttt tatttcaggg
      361 agcattgcac atgtagccgg cgcatcagcc aaaacccttc ctccgtgaga ggataccaca
      421 attccgtagg cacctgcctc ataggccttt actgcacctt ttgcagtcat tactccttta
      481 acgatgaaag gaatttttgt tgattcagtt aattctttaa gctcttcaac ggtttttgca
      541 gaaacaggct tgcctgccaa tgccaaatta ataagtcctg cagaatcaat gtccattgca
      601 aatgccatgg cattaacttc ttcaatcatt ttgatttttt ccaatacctt gtcgtttttc
      661 caaggtttaa gtgtaggcac tgctcttcca tcagcttcct ttataatagg caggctgtcc
      721 aggaataagt tgtctgcagg accatctcct gtaaatcctg cattccctgc ctttaatgtg
      781 cccttaacta ttgcctctat atattctgct tccgttatgg aaccgttgta atttaaactc
      841 ataccaccaa ttggagctgc aaagaaagga tatttgaaat tccttccaaa caaggaaaca
      901 gaagtatcct gtccttcatt tttatagatt gtatccatat tgattttaac tgatgttaaa
      961 aagtccaggc atattttaaa cgcattgccg tttcccttgc cgccggtacc cggaatttcc
     1021 cccttgcatg ccagaccgtt gcattccttg caaaccctgc agtttggcgc cattttttct
     1081 cttgccctgt ctaataattc gttataattc atattcgcct ccccatttgt tccaatcttt
     1141 caagcacctt ttccatcatc agctcatatt tttgaagctt ttctttttct tcatttacaa
     1201 cactttcagg tgccttgcta ataaattttt cattgtttaa tttacccttg actcttttta
     1261 gttcatcttc aagtcttgcc ttttcttttt caagtctttc gatttctttt tcataatcta
     1321 ttaaatcagc taaaggcagg taaatttctg ttccgtcaat aactgaagaa gctgtatctt
     1381 cgcctatgtc tttcttgtta tttacaagtt taatttcagt aatatttgcc aatgttgaaa
     1441 aatattttgc tcctgatgct ataaattgtt ttgcttcttc gtttgaaggt ataaatattg
     1501 cccttgcttt tcttgaaggc agaacattca tttctgctct tatattacga atgctcctaa
     1561 ctgcttccat tataaactta agtgcctctt ctgcttcttc aaagatttct tctttcgaat
     1621 attcaggcca atctgccctt accagccttg aagaagtatc cggcaggtag gaccatattt
     1681 cttctgttat aaatggcata aatgggtgca aaagcttaag catatccttt aaaaccttaa
     1741 tcagcacata tctggctgca tccttgtcat caccgtctgc accgtaaagt ctcggcttaa
     1801 caatttcaat ataccagtcg cagtattcat tccatataaa gtcataaacc ctttgtgcag
     1861 ccattccaag ttcaaatttt tcaagataat ctgtagcttc ctttgctgcc ttatttgctc
     1921 ttgaaattat ccatttatct tctgtcttat agttcttctt tgctgtttct ttatctgtta
     1981 atgcaccatc taaattcatt aatacaaatc ttgaagcatt ccacagttta tttgcaaagt
     2041 ttcggcttga ttccaatctg tcataattaa aacgggtgtc gtttccggga gatattcctg
     2101 tcataagcat gaaacggaga gcatctgcac cgtaattatc gataacatca agaggatcta
     2161 taccgtttcc caaggatttg ctcatttttc tgccttctgc atctctcacc aggccatgta
     2221 taaatgtata tttaaaaggt gacttaccgg taacttccaa agcagaaaat accatcctga
     2281 taacccagaa gaatataata tcataccctg taactaagac atcggtagga taaaaatatt
     2341 ttaagtcatc ggtttcgtga ggccagccca aggttgaaaa tggccatagt cctgacgaaa
     2401 accatgtgtc aagaacatcc tcgtcctgtg tgatgttggt gcttccgcac ttactgcatt
     2461 tttcgggaga atatttttga accattattt cattgcagtc ctgacagtag tatgcaggta
     2521 tcctgtgtcc ccaccacaat tgtcttgaaa tacaccagtc tcttatattt tcaagccagt
     2581 gattgtatat ctttgtaaaa cgctcaggaa ccagctgcat ttccttgtct tcaactacct
     2641 ttaatgcagg ttctgccagt ggtttcattt ttacaaacca ctggtcggaa attctgggtt
     2701 caatatcatt gtggcatctg taacaggttc ccacattgtg ttcggtattt tctattttaa
     2761 gtatgaagcc gctatcatca aggtccctta caaatgcctc cctgcagtca taacggtcca
     2821 ttccttcata tttgcccgca atttcattca tagtcccgtc ttcattcatg cagcttaatt
     2881 ggtcaaggtt gtggcgaagt ccaatctcaa agtcgttagg gtcatgagca ggagttattt
     2941 tcaatgcacc tgttcccttt gacctatcag ggtattcatc ggcaataatc ggaatttctc
     3001 ttccaaccaa aggaacaatt actttccttc cgattaaatg tttgtatctt tcatcatcag
     3061 gatgtgcagc tatggcaacg tctccaaaaa ttgtttccgg tctggtggtt gcaattataa
     3121 aaaattcatc cttattatca gcaaagggat attttacata ataatatttg ccggcagtgt
     3181 cctcatgttc aacttctgca tcagaaagtg ttgtatggca gtcaggacac cagtttataa
     3241 ttctgtttcc cttataaata agtccccttt cgtacagcct tatgaaaaac tcagtaacag
     3301 cttcattgca gccttcatcc atggtgaatc tttctcttga ccagtcgcag gaattaccaa
     3361 gctttttaac ctggtcaact atcatgccgc caaattcttc cttccacttc caggctctgt
     3421 taagaaattc ctctcttcct atatcttcct tggtttttcc ttcttccttc ctaagtcttt
     3481 caactacttt tacttcagtt gctatgctgg catggtctga acctggaagc cacaaggctt
     3541 catagcccat cattcttttc catctgatga gaacatcctg caatgtttgg tccaaggcat
     3601 gccccatatg aagctggcct gtaatatttg gaggcggcat cacaatggta tatggtttct
     3661 tgtctttgtt aatttctgct ctgaagcagt tttcatccat ccagatttta taaattttat
     3721 cttcaaattc cttcggatta tacttacttg gtatgtttgt tgacataata tccttccttt
     3781 ctaaaataat cgaaatatgc tcatgcttca tcgccaattt gtacgctcct ttcgtcgcgc
     3841 aaattgagat ttcgctgcgt ctgacctacc atccatttca tatatttggg gacattcctc
     3901 atgacctcaa agacttgcta catagtagag gtgtgtcccc atacctattt tgtcgaagaa
     3961 attgggttag gtcataaaaa aagcgggttt tctccatcaa aaggacgaga aaacccgcgg
     4021 taccacctaa tttccattac ggctcttaaa ctatataacg gtaatccgtt taatcttact
     4081 gttatttcag attaaatgct caaaagctac cttcagtaca tattgaccta tgaagacttt
     4141 catccggtaa tcttcactct ctggcaagtt tatgcactta ctcctctttt tcatcgcagc
     4201 tattcaatta catatcataa tattaaaaat atatgttatt gtcaacattt tttttatatt
     4261 tacccctatt ttgcttattc tttgcctatt gcttatttgt acttattgct tatttgctat
     4321 tttcctattt gacaatacca gtttaatgta atatacttta atcaattaaa catctttcga
     4381 tattggtgcg cttaaatgcg ataataggga attgggttag aatcccaaac agtcctgctg
     4441 ccgtaatgag agagcggttt ttatgctgat gcaaccactg gatatatccg ggaaggttaa
     4501 aaacctgcta tgacctctga gtcggaaaac ctgcc
//
LOCUS       DAOPUT010000108         4512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_19321_length_4512_cov_19.133274, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000108 DAOPUT010000000
VERSION     DAOPUT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19321_length_4512_cov_19.133274"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PKK75_07595"
     CDS             complement(<1..584)
                     /locus_tag="PKK75_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HNZ83501.1"
                     /translation="MKAIIVEIKGNHAAILSDDGVVSKIRNKDYYIGQEIIINNNSGR
                     LIRMTAAAAAAIMIFATPAWAYLTPYSYVSVDVDPSFEFFINRFDRVLTVRAYNDDGK
                     ELSKDINIDGLKNKEIQSAVRSVLIELKNKGYIIEGEEDGVIVAALSKSEKKTNMLSE
                     KLRTAFYEEVTLKQTDTEIQANQNPGLEKPEKPE"
     gene            complement(565..1296)
                     /gene="sigI"
                     /locus_tag="PKK75_07600"
     CDS             complement(565..1296)
                     /gene="sigI"
                     /locus_tag="PKK75_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HNZ83502.1"
                     /translation="MSDLDQIAIEAKNDADSMNKLIAQYENFMLKCASSAARRYISKS
                     DDEWSIALSAFTEAIENYSAGKGSFLNFAELVIRRRMIDFMRSKSRYAHEISVNPSLF
                     DTDSQDEEEYSMQSEIVKKVSIAEDNSTKMEIALINEIFSDYGFSFMDLVQCSPKSQK
                     TKRSCAKAVAYLIKNPIIINEIKIRKQLPLNIIEKSTKVPRKMLERHRKYIIAAVEIM
                     SGDFPNLAGYLEYIRRELEDESNNS"
     gene            complement(1390..1629)
                     /locus_tag="PKK75_07605"
     CDS             complement(1390..1629)
                     /locus_tag="PKK75_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83503.1"
                     /translation="MKIINEPIKVMAVFNTDGKIEPVKFRIDDKVVRIEKIMKTYEEN
                     IVGNKRIVFVCLHKGKDIFELKYELDTKTWYLFKK"
     gene            complement(1639..2877)
                     /locus_tag="PKK75_07610"
     CDS             complement(1639..2877)
                     /locus_tag="PKK75_07610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNZ83504.1"
                     /translation="MGHIIMHIDVNSAFLSWTAAYEKQMGIERDIRTVPAVIGGNEKS
                     RHGIVLAKSTPAKKFKIQTGMSLMEARQMCPDLLVISPDYNIYVRASKALREYLKKYT
                     PDVEVFSIDECFLRFTDINRDQAVKLAYKIKDGVRDKFGYTVNIGISENKLLAKQAGD
                     LEKPDKCHTMFIEEIKEKLWPLPVEELFMVGRRTRPKLNRWGIYTIGELANADYKLIA
                     AMLKSHGRLIYNYAWGRDISIFKEKDPIKSVGNSSTLKFDITDRETAHVVLLSLTEMT
                     AWRLREAGMYCRVVSISIKDKDFGFKIKQRKIMYFTDCTRDIYMNACSLFDELWDNKP
                     VRALGVHVSDLEFSSFKQINFFQDELSLKNEILDKTVDEVRAKYGDNSIIRGVFANTD
                     LKPVLGGYPDDEYPGMTSIL"
     assembly_gap    3065..3164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3165..3889)
                     /locus_tag="PKK75_07615"
     CDS             complement(<3165..3889)
                     /locus_tag="PKK75_07615"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNZ83505.1"
                     /translation="MIELTVRSESMVLVVDIGNTNIVIGVYKGNELVGNWKMVTKSEK
                     TSDEYGISIVNLLSYNGIDHKNISSAIVSSVVPNVMHSFENTLKKFFNIEPIVVGPGV
                     KTGITIMIENPREAGADRIVGLVAAHELYSKGDTTIVIGFGTATTYDVINEKGEYKYG
                     ITSPGIQISADAMWQRTAQLPKIEIKKPDTILAKNTVASMQAGLVYGYIGQVEYIIKK
                     IKEELHNDNIKVVATGGFGRLIS"
     gene            complement(3892..4422)
                     /locus_tag="PKK75_07620"
     CDS             complement(3892..4422)
                     /locus_tag="PKK75_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNZ83506.1"
                     /translation="MREREVKVLNIDKEEIEKKLIDIGAVLVKDEDQINYRFDTDDYY
                     LKKTYDAYLRIRITKNNLSGETINTMTFKRNLKRDSLRINEEVNTDLSNWESAAKIIE
                     ILGYKQKRPGYKHRRSYMYDNILFEIDTWDEETYAKPYLEIEVSSDEELEKAIMILNI
                     DRSQITTKPIDEMRKD"
BASE COUNT         1168 a          969 c          639 g         1636 t
ORIGIN      
        1 ttctccggtt tttcaggttt ctcaagccca gggttttggt ttgcttgaat ttcagtgtct
       61 gtttgtttca gtgttacttc ttcatagaag gcagtccgca gtttttcaga aagcatgttt
      121 gtttttttct ctgatttgct caatgctgca actattaccc cgtcttcctc gccttcaata
      181 atataaccct tattttttag ttcaattaac acacttctta ctgcactttg aatttcttta
      241 tttttcaaac cgtcaatatt tatatctttt gacaattcct ttccgtcatc attataggcc
      301 ctcacagtaa gcaccctgtc aaatctgttg ataaagaact caaaggatgg atccacatct
      361 acgctgacat atgaataagg ggtcaggtat gcccaggccg gtgtggcaaa gatcatgatt
      421 gcagcagctg cggcagctgt catcctgatt aaccttccgc tgttattatt tattattatt
      481 tcttgaccta tgtaatagtc cttgtttctt attttagaaa caaccccgtc atctgacaat
      541 attgctgcat gatttccttt aatttcaact attattgctt tcatcttcca actcccggcg
      601 aatatattcc aaataacctg ctaaattcgg aaaatctccc gacattattt ccactgctgc
      661 tattatatat tttcgatggc gttccaacat tttacggggt acctttgtgc ttttttcaat
      721 tatatttaag ggcagctgct tcctgatttt tatttcattt attattatgg gattttttat
      781 taaataagca acggcctttg cgcaggacct ttttgttttc tgagatttag gcgagcattg
      841 gaccagatcc ataaatgaaa aaccataatc ggagaatatt tcattgataa gggcaatttc
      901 catcttagtt gaattatcct cagcaatgga gacctttttt acaatttcag actgcatgga
      961 gtattcttct tcatcctgtg aatcagtatc aaacagcgag ggatttactg aaatttcatg
     1021 tgcatacctt gacttgctgc gcataaaatc aatcatccgc cttcttatta ctaattctgc
     1081 aaagttcaaa aaactgcctt taccggcaga ataattttca attgcttcag taaaagcaga
     1141 caaggctatt gaccactcat catcactttt tgaaatatat cttctggcag ccgatgacgc
     1201 acatttcagc ataaaatttt catattgagc tatcagctta ttcattgaat ctgcatcatt
     1261 tttggcttca attgcaattt ggtccaaatc actcatatac ttaccctcta ttagctgcaa
     1321 ttacagatta ttataacatt cagaccgtat tattacaata tttcaaatct tacaaatttc
     1381 ttaatccccc tacttcttaa ataaatacca ggttttcgta tccagctcat atttaagttc
     1441 aaaaatatcc ttacccttat ggaggcatac aaatacaatc cttttattgc caacgatatt
     1501 ctcttcatat gtcttcatta tcttttcaat tctcacaact ttatcatcaa tcctgaattt
     1561 tacaggctct attttcccgt ctgtattaaa tacagccatt acttttatag gctcatttat
     1621 tattttcata gtccacctct ataaaatact ggtcatgccc ggatattcgt catcgggata
     1681 tcctcccagg acaggtttta aatcagtgtt tgcaaaaacc cctcttataa ttgaattatc
     1741 accgtactta gcccttacct catctacagt cttatctaat atttcattct ttaagcttaa
     1801 ttcatcctga aaaaaattaa tctgcttaaa acttgaaaat tccaagtctg atacatggac
     1861 tcccaatgcc cttacaggct tgttatccca caattcatca aataagctgc atgcattcat
     1921 ataaatgtct cttgtacagt ccgtaaaata cataattttt ctctgcttga ttttaaaacc
     1981 gaaatccttg tccttgatac ttatgcttac aaccctgcag tacatacctg cttctctcaa
     2041 tctccaggca gtcatttcag tcaggctcag cagtacaaca tgggctgttt ccctgtctgt
     2101 aatatcaaat ttcaaggtgc tgctgtttcc tacgctcttg atcgggtcct tttctttaaa
     2161 gattgaaata tctctccccc aggcataatt gtaaattaat ctgccgtggg atttaagcat
     2221 ggctgcaatt aatttataat ctgcatttgc aagctctccg attgtataaa ttccccatct
     2281 gtttaattta ggtctggttc ttcttcccac cataaataat tcctcaaccg gcaggggcca
     2341 caatttctct tttatctcct caatgaacat ggtatggcac ttatcaggct tttccaagtc
     2401 tcctgcctgt tttgcaagaa gcttgttttc agaaattcct atatttactg tatagccgaa
     2461 tttatcccgg acaccgtcct ttattttata ggcaagctta acagcctggt ctctgtttat
     2521 gtccgtaaac ctcaggaagc attcatcaat gctgaaaact tccacatcgg gagtatattt
     2581 tttcaaatat tctctcaaag ccttgcttgc tcttacgtaa atattgtaat ccggagatat
     2641 tacaagcaaa tcaggacaca tctgccttgc ttccataaga gacattcctg tctggatttt
     2701 gaatttcttg gcaggagttg attttgcaag aacaattcca tggcggcttt tttcatttcc
     2761 gccgataaca gccggcactg tccgtatatc tctttctatt cccatttgtt tttcatatgc
     2821 agccgtccag cttaaaaaag ctgaattcac atcaatatgc attattatat gccccatcaa
     2881 ctccgcctcc ttgctgaaac aggtaccttc caggtgtctt ttttgctgcg gcacgtgaat
     2941 atcccagctt gtttttttgt ttttcgaaca tttgttctat tattatacca caatttttaa
     3001 attttaaaag ccttttttga aatttttctt taaatttggg ttacataaaa aaagttctgt
     3061 aaacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcggat ataagccttc
     3181 cgaacccccc ggttgccaca acctttatat tatcattatg caattcttct tttatttttt
     3241 taataatata ttctacctgt cctatgtatc cgtaaacaag ccccgcctgc atgcttgcta
     3301 cagtgttttt tgcaagaatt gtatccggct ttttaatttc aattttcgga agctgtgcag
     3361 ttctttgcca cattgcatcg gcagaaatct gaatccccgg agatgtaatg ccgtatttgt
     3421 attctccttt ttcatttata acatcatagg ttgttgccgt tccaaagcct atgactatcg
     3481 tggtatcgcc ctttgagtat aattcatggg ctgccacaag ccccacaatc ctgtcggctc
     3541 ctgcttctct tgggttttct atcattattg taatccctgt tttcactccg ggtcctacga
     3601 caatgggctc tatgttaaaa aactttttga gggtattttc aaatgaatgc attacattgg
     3661 gcacaacaga agacactatt gcactgctga tgtttttatg gtcaatgcca ttgtaactta
     3721 ataaatttac tattgaaatt ccatattcat cggatgtttt ttcactcttg gtgaccattt
     3781 tccaattccc gacaagctca ttgcccttgt aaacaccgat tactatattt gtattgccta
     3841 tatcgacaac taaaaccatg ctttcactcc taaccgttaa ttctatcatt cctaatcttt
     3901 cctcatctcg tcgatgggct tggttgtaat ctggcttctg tcaatattca atatcattat
     3961 tgctttttcc aattcttcat cagaggaaac ttcaatttca agataaggtt ttgcgtatgt
     4021 ttcttcatcc caggtatcaa tttcaaacaa tatattatca tacatatagg acctgcgatg
     4081 tttgtagccg ggcctttttt gtttataacc aagtatctcg attattttag cggcactttc
     4141 ccaattggaa aggtctgtat taacttcttc attaatcctt aaggaatccc tttttaagtt
     4201 tcttttaaaa gtcattgtat taattgtttc acctgacaaa ttatttttag taatccttat
     4261 tctcaaataa gcgtcgtagg tttttttcaa ataataatcg tctgtatcga atctgtagtt
     4321 tatttggtct tcatccttta caagaacagc tccaatatcg ataagctttt tttctatttc
     4381 ttccttgtct atatttaaga ccttaacttc ccgctctctc atttcgccct ccgcctaaat
     4441 ttatgtaatt ataacatata tatcgaaagt tttaaacatt tattattgga tgtgcaaact
     4501 atttgaatat tg
//
LOCUS       DAOPUT010000109         4447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_19713_length_4447_cov_18.247040, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000109 DAOPUT010000000
VERSION     DAOPUT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19713_length_4447_cov_18.247040"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKK75_07625"
     CDS             complement(<1..557)
                     /locus_tag="PKK75_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ83507.1"
                     /translation="MLSQEMIKLGTQRSVIRELFAYGLQRAELVGPENVLDFSLGNPS
                     VPAPKEVQEAIIDIVKNNDPMVYHGYSTSQGHASCRKAIASNLNKRFDRAYSPNDLFI
                     TCGAAASLCITFKALAADPSDEIIVLAPYFPEYKVFIEGQGARMNLVPNRNDMKLNLE
                     GIKEAVNKNTKALLINTPNNPSGVV"
     gene            complement(639..941)
                     /locus_tag="PKK75_07630"
     CDS             complement(639..941)
                     /locus_tag="PKK75_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83508.1"
                     /translation="MIIFLNLGSLIFGLIAWVLPVINILKYKKCDKWYVLSISSISFC
                     AVSIFFQLYYQNYLVKIDDLPALMDTINTSSFLSAVLLLVTLILNAINLTIFLLKD"
     gene            complement(953..1198)
                     /locus_tag="PKK75_07635"
     CDS             complement(953..1198)
                     /locus_tag="PKK75_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83509.1"
                     /translation="MKRILIILSNFIMSIGVIAVISPMILYWFIHGDYERYIWIINGP
                     FPFSSFGGGPFQMLMYISLFITGAVLIMVSLIIKRKY"
     gene            complement(1293..2789)
                     /locus_tag="PKK75_07640"
     CDS             complement(1293..2789)
                     /locus_tag="PKK75_07640"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNZ83510.1"
                     /translation="MDSKIKTKLSLYGIISALTFIYLILTDNPRISVPIFFVIQGVVL
                     FYILKNMEETTNIKGLLILIPIFIISLNNFISANYMFMPVNFLVIVFLYSLMFLLLGN
                     RLNMLKLSIYSILKTIVNLFVPIINFIIPFKWIGERSKNKDKNILTVRIALGIAISLP
                     SVIFLVMMLSNADMVFNNSFIIFNNWLKKLFEFISIYKIVIGAFAGLYLFGHLYSVLI
                     KDEKIFNIINEKTYRANIKGDVIVFNILLVSILIVYTIFIIIQFKYLFSGGALPNGLN
                     YSEYARRGFFELVFLSVLNIALILLTTYLLKDKIYEDKNKWALFTKLMLVYLCVITGI
                     LLISSYYRMSLYDSAYGFTRLRVMVYIFLVFEMIGLLATLVYIARHNFNILTVYAAVF
                     LAFYLTLNLVKIDELIAKRNVDMYLSGQAEDIDIDYLMTLSPDALPQIMRLTDSNVQY
                     VTRIKTINHLKEMSVYYAQMGNNWQSYNLSIERNKSLLEENMSRWEYK"
     gene            complement(2779..2997)
                     /locus_tag="PKK75_07645"
     CDS             complement(2779..2997)
                     /locus_tag="PKK75_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ83511.1"
                     /translation="MAIIIQLDVMMAKRKIGLMELAEKINITPANLSILKNNKAKAIR
                     LSTLDEICRALDCQPGDIIEYVEEENGQ"
     gene            complement(3008..3460)
                     /locus_tag="PKK75_07650"
     CDS             complement(3008..3460)
                     /locus_tag="PKK75_07650"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HNZ83512.1"
                     /translation="MYQKSIVHYMTKISIDILFIVAIICCILVPFSSSWLFRYFGIVE
                     EGALFVKAILMASGISCVYILWQLKVIFKTLLQGNPFIHANVSSLRKIAVACLVIAFI
                     YFVKMIVMPTISTIIIIIIFVVACLLCLTLKDLFKQSIYYKDENDLTV"
     gene            complement(3723..4190)
                     /locus_tag="PKK75_07655"
     CDS             complement(3723..4190)
                     /locus_tag="PKK75_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNZ83513.1"
                     /translation="MTNSFSGIEMLKIAILMEDEGYMFYKNGADNTTGQLKEFLLTCA
                     DQELSHKEKFAKMYRELEAKDEDSAEYLYGDEVSGYLRSLIENQVFKKDEDFDDAFLD
                     IKTAAKNAYDTEIRTIDVYTVMYEGLTDEEVKNTMSLIIDEEKAHAASFEKLM"
BASE COUNT         1507 a          773 c          634 g         1533 t
ORIGIN      
        1 tatactactc ctgaaggatt gttgggtgta ttaataagca gtgctttggt atttttgttt
       61 acagcttctt tgattccttc cagattaagt ttcatgtcat tccggtttgg aactaaattc
      121 atccttgcgc cttggccttc tataaagact ttgtattcag gaaagtaagg agccaatact
      181 ataatttcat ccgaagggtc ggctgccagt gctttaaaag taatgcataa tgatgcggca
      241 gctccgcaag tgataaacaa atcattcggg gaataggctc tgtcaaacct cttgtttaaa
      301 tttgaagcaa ttgcctttct gcagcttgca tggccctgtg atgtgctgta tccatgatat
      361 accatggggt cattattctt cacaatgtca atgatggctt cttgtacttc cttgggagcc
      421 ggtacacttg gatttcctag actgaagtcc aggacgtttt ccggaccgac aagctctgct
      481 ctctgcaagc cataagcaaa taattctctt atgacagacc tttgtgtacc taatttaatc
      541 atttcttgtg ataacatttc ttcctccaat aatctatagt ttttcgctac tgtcattata
      601 ttaacatact ttataaaaaa agtaaacgtt attaaatttc agtctttcag taaaaatatg
      661 gttaaattaa ttgcatttaa tataagagtt actaaaagaa gaactgctga caaaaaagaa
      721 gaagtgttta ttgtatccat taaggcaggc aaatcatcaa tttttactaa ataattttga
      781 tagtaaagct gaaagaatat agatacagca caaaaactaa tgctggaaat cgataggaca
      841 taccacttat cacatttttt atacttaaga atattaataa caggtaatac ccaagcaatt
      901 aagccaaata ttaaactgcc taaattaaga aaaataatca ttgctctcct ctctaatact
      961 tccttttaat aatcaatgaa accattatca atactgctcc ggttatgaat aatgaaatat
     1021 acattaacat ctgaaaggga cctcccccaa agctgctaaa cgggaaaggt ccgttgatta
     1081 tccatatgta tctttcatag tctccatgta tgaaccaata cagaatcatc gggctgataa
     1141 cagcaataac accaatgctc ataataaaat ttgataaaat aattaaaatt cttttcatga
     1201 aaaccgcctg ctaagactaa ctgtggatta aaacatcaaa tctatcaatt ctatactcaa
     1261 aggtgaaaac atgagtagag aaaatactgt ttttatttat attcccatct actcatattt
     1321 tcttccaata aactcttgtt tctttcaatt gataggttgt agctttgcca gttatttccc
     1381 atctgagcat aatacacgct catttctttc aggtgattaa ttgttttaat tcttgtcaca
     1441 tactgaacat tgctgtcagt aagacgcatt atctgaggca gggcgtccgg tgacaaagtc
     1501 attaaataat caatatcaat atcttctgcc tggccgctta aatacatgtc aacatttctt
     1561 tttgcaatca gctcatcaat tttcacaagg ttcagtgtca gataaaatgc aagaaaaaca
     1621 gcagcgtata cagttaatat attaaagttg tgccttgcaa tatatactaa tgtcgccagt
     1681 aaacctatca tttcaaatac taagaatata taaaccataa ctcttaatct tgtaaaaccg
     1741 taagcactgt cgtaaaggga cattctgtag taagaagata tgagtaatat ccctgttatc
     1801 acacaaagat aaacgagcat aagcttagta aataaagccc acttattttt gtcttcgtat
     1861 attttgtcct ttaacaggta tgtagttaat aaaattaaag caatatttaa aacgcttaag
     1921 aacactaatt caaaaaaacc tcgtcttgca tattcagaat aattaaggcc attaggaagt
     1981 gctcctccgg aaaataaata cttaaactgt attatgatga aaattgtata tacaattagg
     2041 attgagacta acaatatatt aaaaacaatt acatcgcctt ttatattagc cctgtatgtt
     2101 ttttcattaa ttatattaaa aatcttttca tcctttataa gaacggaata tagatgacca
     2161 aataaataca atcctgcaaa ggctccaatt actattttat atatactaat gaattcaaaa
     2221 agctttttga gccaattatt aaatatgata aaactgttgt taaaaaccat gtctgcattg
     2281 gacaacatca ttacaagaaa tattaccgaa ggaagggata tggcaattcc taaagctatt
     2341 cttaccgtaa gaatgttctt atctttattt tttgaccttt cacctatcca cttgaaggga
     2401 attataaaat taataatagg tacgaaaaga ttcactatag tttttaaaat actgtatatg
     2461 cttaatttca gcatattcag cctattgcct aaaagcagaa acatcagact gtacagaaac
     2521 actataacca ggaaatttac aggcataaac atataatttg cacttatgaa attatttaag
     2581 gatattatga aaataggaat cagtattaat aatcctttga tgttggttgt ttcctccata
     2641 ttttttaaaa tataaaataa aacaacccct tgtattacaa agaatatggg aacacttatt
     2701 ctcggattat ctgtgagtat caagtatata aaagttaaag cagaaataat tccgtaaagt
     2761 gataattttg ttttaatttt actgtccatt ctcttcctcc acatattcta taatatcccc
     2821 gggctgacaa tcaagggctc tgcaaatttc atccagggtt gaaaggcgga ttgcttttgc
     2881 cttgttgttt ttaagtattg aaagattggc aggagttata tttatttttt cagccagttc
     2941 cataagaccg atttttcttt tagccatcat aacatcaagc tgtataatta tagccatcta
     3001 atccctccta aaccgttaaa tcattctcgt ctttataata aattgactgc ttgaacaaat
     3061 ccttcagagt taagcataac aaacatgcaa caacaaatat gattattatg ataattgttg
     3121 aaattgtagg cataactatc attttaacaa aatatataaa tgcaatcacc aaacatgcaa
     3181 cagcaatttt tcttaaagat gacacatttg cgtgtataaa tggattgcct tgcagcaagg
     3241 tcttaaaaat aaccttaagc tgccataaaa tataaacgca tgatatgccg gaagccatta
     3301 gaattgcttt tacaaaaagt gcaccttctt caactatccc aaaatagcga aacagccagg
     3361 atgatgaaaa aggcaccaag atacagcaaa taattgccac tataaacaaa atatcgatgg
     3421 aaattttagt catataatgt acaatgctct tctgatacat aaaatcctcc tgaatgtaat
     3481 tgtaacttaa ttatagcaga tatttttcgc atgtcaatat atttttattg tttctcgata
     3541 atttattatt gtattttgtt aatcctgttc ttattttatg tgtttgctaa ttcgtctttg
     3601 atagatgttt aaaaagattt gcagtaaata aaagtttaaa aatcggtata taaaaatatc
     3661 agcgccgata ctgtacatgg cgctgaattt aaatagcttt atatatattt tgattatatg
     3721 tgttacataa gtttttcaaa agatgccgcg tgagcttttt cttcatcgat tataagagac
     3781 atggtatttt ttacttcttc gtcggttaat ccttcataca taaccgtata aacatcaatt
     3841 gttcgaattt ctgtatcata agcattttta gccgctgtct ttatgtcaag gaatgcatca
     3901 tcaaaatcct catctttttt gaaaacctga ttttcaatca gtgatcttaa gtagcctgaa
     3961 acctcatcgc cgtagaggta ttctgctgaa tcttcatctt tagcttccaa ttccctatac
     4021 atctttgcaa atttctcttt gtgtgataat tcctgatcag cacatgttaa taaaaattcc
     4081 tttaattgac ctgtcgtatt atccgctcca ttcttgtaga acatatatcc ttcatcttcc
     4141 atcaatattg cgatttttaa catttcaata ccgctaaaac tatttgtcat aaatttatcc
     4201 tcctattatt tatgtccgtt aatgaaatta tactagcaac ttattcattt ttcaaccaat
     4261 attaaaacaa taattttttt attttacaaa agaaatgtat tcggatgttg attaagtctt
     4321 gcttctttta atgtaccggt ttgaataaaa tcagctttta caagaaactc agctgctggt
     4381 ttttatcaag aattttttta atgaatgttt ttctgtgtat ttcagaaggt ccgtattcaa
     4441 gtaaggc
//
LOCUS       DAOPUT010000110         4437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_19779_length_4437_cov_14.629621, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000110 DAOPUT010000000
VERSION     DAOPUT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19779_length_4437_cov_14.629621"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1480
                     /locus_tag="PKK75_07660"
     CDS             332..1480
                     /locus_tag="PKK75_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNZ83514.1"
                     /translation="MIKNKNLLSGLKILTLIVLFLSALYFENAQQQRLLVLILIFTLF
                     LANNAAKFFVKTQNKLFILFLADIALIYILESNSRLLINYFFHSFYIIIFLEASLLLP
                     LKKGITIGIITVVISMIKYAYLIYYKFNLSNVSQMVFFLMVNILILVIATFAQHTKEE
                     KEKKDILYRELLDTHKQLKEYTDELNRLSVIEERNRIARDIHDTLGHNMTALIMQLQM
                     AGHYLKSDEKKSTEMLSSSLNTARDSLSKIREVVETLRGKETSPARTIKILAEEFSEK
                     TGAEIELNIEGEISNNNSANSAIYHILQEGMTNAVRHGNASKIRVNLIYSNENIKFSI
                     KDNGQGALVLIEGFGLKGIRERVEAFGGKVEFKSEDGFSIAGEIFTGK"
     gene            1621..2268
                     /locus_tag="PKK75_07665"
     CDS             1621..2268
                     /locus_tag="PKK75_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ83515.1"
                     /translation="MIKLLLADDQDILTEGLKLILGAEEDIEIVGTANNGNKAYDLCR
                     VRKPDVVLMDIQMPEVNGVGATAMIKKEFPHIKIIVLTTFNDDEYIYDALKNGASGYL
                     LKDTSPMEILKAVRTVYKGGALIQSEVAVKVIDRFSQLAKDNADKHTDPKVQLLTDRE
                     IEICRLIAEGKNNQEISEKLFLSQGTVKNHITRVLLKLDLRDRTQLAIFTINNDL"
     gene            2709..3746
                     /locus_tag="PKK75_07670"
     CDS             2709..3746
                     /locus_tag="PKK75_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83516.1"
                     /translation="MKRKIVFIAAFLLILMMFITACNTNNLVEYKKASEKTDQIVKGQ
                     TAGEFITTTEINQDGLTAEEIKELNYIKDMNGSFSVVFDDEAEKSIIRSYMNFGGLGY
                     DFEVYINKEEVSIKLPVIGKYLRIDEDMVSEGEEYYDSENQIISEETGKEFAKRWLSL
                     MNDEDVFKGKNIVLTTPDGEVKTTEYTIKLSDDQIKTLLRDSAEILSNDESLKSFYEK
                     NISSNIKKIENDLEQKTNGSEQKTFKEFLNDIVNSAEKFKVENFSYTALVDIDGYIVN
                     EIIDISIKAIDAEKEGIISANYRLDIKTWDINKDQKFEFPVLTDENTIKPDEMNENMP
                     SVMEDYFSIEI"
     gene            3756..>4437
                     /locus_tag="PKK75_07675"
     CDS             3756..>4437
                     /locus_tag="PKK75_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012958969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ83517.1"
                     /translation="MLKVENLYKNFGNIRAVDGISFEVERGEVFGLLGPNGAGKSTTI
                     SIISTLLPPTKGEILFEGESIFKNSKNLRQKLGIVPQDVALYPTLTGYENLRFWGNLY
                     GLKGADLKKRINEVAEIIGLNGRLKDKVDKYSGGMKRRLNIGAALLHEPELLIMDEPT
                     VGIDPQSRSHILDTVLKLNAQGMTIIYTTHYMEEAEELCSRICIMDEGKIIASGTKQE
                     LVELVKEKT"
BASE COUNT         1642 a          662 c          930 g         1203 t
ORIGIN      
        1 gaagaactgg gaatggaaga tgtagcagaa aaaatataca gcttgaatta ttagacagaa
       61 ttttcaacct agcgcagcaa agagtctcat cttaatttga gacatttgtc atggtggaga
      121 cggttctttt tgttcttggc aacaagaaga gccgtttttt attttaattt tagggggatt
      181 ctttaaaatt ttgccaagga gaactgccat ctccggtccg caagaacgaa agaaccgtcc
      241 taaaatatac atatgcaaaa aattaattat atggtaatat atgattgcaa ttaatgctga
      301 atgtcaatca tccacaactt gcaggtgtta tatgataaag aataagaatc ttctatccgg
      361 attaaaaatt ctaactttga tagttttatt tttatcagca ctgtattttg aaaatgcaca
      421 gcagcaaaga ctgttagtgc tcattcttat ctttacgctt tttttagcca ataatgcagc
      481 taaattcttt gttaaaaccc aaaataaact tttcatatta tttcttgccg atatcgcact
      541 tatttatata ttggaatcca attcaaggct tttaattaac tacttcttcc actcttttta
      601 cataattatt tttcttgaag catctctttt gttgcctcta aaaaaaggaa ttacaatagg
      661 aattattacg gttgtcatat ccatgataaa atacgcatat ttaatttatt ataaattcaa
      721 tttgtccaat gtatctcaga tggtattttt tttgatggta aatatattga ttctggtaat
      781 tgctacattt gcacagcata ccaaggaaga gaaagaaaaa aaggatatcc tgtacaggga
      841 acttttggac acacacaagc agcttaagga atataccgat gaattaaaca ggctgtctgt
      901 aattgaagaa agaaacagga tagcaagaga catacatgat acattggggc acaatatgac
      961 agccttgata atgcagctgc agatggcagg acattattta aagtccgatg agaaaaaatc
     1021 aacagagatg cttagcagct ctctaaatac tgcaagagac agcttgtcta aaataagaga
     1081 agttgtggaa acattaagag gtaaagaaac atcaccggca aggacaatca aaatcttagc
     1141 cgaagaattc tccgaaaaaa ccggtgcaga aatagagctt aatatagagg gagaaatttc
     1201 aaataacaac agtgccaact cggcaatata tcacatattg caggaaggta tgacaaatgc
     1261 agtaaggcac ggcaatgcat ctaaaattcg ggttaattta atatattcca atgaaaatat
     1321 aaaattcagc ataaaagata acggacaagg agcattggtt ctaattgaag gatttggtct
     1381 gaaaggaata agagaaagag ttgaagcatt tggaggcaag gttgaattta aatcagaaga
     1441 cggattttct atagctggag aaatcttcac cggcaaatag aggggaatct tataaatatt
     1501 taaaatatga gatcttaaac tgcaattaag atatatggaa tgacaaggac tgcgttccaa
     1561 taatgatatt attgataata ataaaggtta attaatgata tgtgaggagg aggaagttaa
     1621 atgattaaat tactgctggc agacgaccag gatatattga cggaagggct taagcttata
     1681 cttggcgccg aagaagatat tgaaattgtc ggtaccgcta acaacggcaa taaagcatac
     1741 gacctatgca gggtaagaaa gcccgatgtt gtgctaatgg atatacaaat gccggaagta
     1801 aacggtgttg gggcaactgc aatgattaag aaggaatttc ctcatattaa aataatagta
     1861 ctaacaactt ttaatgatga tgaatacatt tacgatgcat taaaaaacgg tgcatcgggt
     1921 tatttgctga aagacacttc tccaatggaa attttaaaag ccgtccgaac tgtctataag
     1981 gggggagcct tgattcaatc agaggttgca gtaaaagtaa tcgacaggtt ttcacagctg
     2041 gcgaaggata atgcggacaa acatacagac ccgaaagttc aattgctgac agacagggaa
     2101 attgaaatat gcaggttaat tgcagaaggc aaaaacaatc aggaaatatc agagaaattg
     2161 ttcttaagcc agggaaccgt caaaaaccac attacaaggg ttttgttaaa gctggacttg
     2221 agagaccgca cccaattggc catttttacc ataaataatg atttataaat atggggacac
     2281 gcctttaatg tgaaaagggg acaaaccttc aatttttaaa ggaatgtccc catactatta
     2341 atgcccataa ttaaagtttt tctctttgct tgaatgacac actctgactt atatgataat
     2401 taccataaaa tgtgccaaaa gtcatatgat aattcataaa tttagtgatt taagatactg
     2461 tttatttgct ttagaatttt atattatgat accagtggga gaagcagtgt aaattgaagg
     2521 tttgtcactc tgaggtcttt agcccgaagg aatcccataa tttataagac gggttcagga
     2581 tggggaacgg atttaggatg acaaagggat taaggaagcg acagcatcag aataacaagc
     2641 gggttcaggt gcaacgcgtt caggatgccg cactgctctc aggatacata ataattggag
     2701 gaatagaaat gaaaagaaaa atagttttta tagctgcgtt tttactaata ttgatgatgt
     2761 ttatcacagc atgcaatacg aataacctag ttgaatataa aaaggcatcg gaaaaaacag
     2821 atcaaattgt caagggccag acagcaggag aatttatcac cacaacggaa ataaaccagg
     2881 atgggttgac agcggaggaa ataaaggaac ttaattatat taaggacatg aacggcagct
     2941 tcagcgtagt atttgatgat gaagcagaaa agtcaattat cagaagctat atgaactttg
     3001 gagggctggg atatgatttc gaagtttata tcaacaaaga agaagtgtcc ataaagcttc
     3061 cggttattgg aaagtattta aggatagacg aagatatggt gtcagaaggt gaagaatatt
     3121 atgattcaga aaatcaaata atttctgaag aaaccgggaa ggagttcgct aaaaggtggc
     3181 tcagcctgat gaatgatgag gatgtattca aaggaaaaaa tatagttctt actactcccg
     3241 acggagaagt taaaactacc gagtatacta ttaaactgag tgacgaccag ataaaaacac
     3301 tgcttaggga cagtgcggaa atcttgtcta atgatgaaag cctaaaatca ttttatgaaa
     3361 aaaatataag ctcaaatata aaaaaaatcg agaatgactt agagcaaaaa acaaacgggt
     3421 cagagcaaaa aacctttaaa gaattcctga atgatatagt aaacagtgca gaaaaattca
     3481 aggttgaaaa tttcagctat acagctcttg ttgatattga cgggtatatt gtaaatgaaa
     3541 ttatcgatat atcaataaaa gcaatcgatg ctgaaaaaga aggaataatc agtgcgaatt
     3601 ataggcttga tataaaaacc tgggatataa acaaggacca gaaatttgaa tttcccgtgc
     3661 ttacagacga aaatacaatt aagcctgatg aaatgaatga aaatatgcct tcagttatgg
     3721 aagactattt cagtatagaa atttaggggt gacatatgtt aaaggttgaa aatttatata
     3781 aaaattttgg aaatataaga gcggtagacg gtatcagttt tgaagtggaa agaggcgagg
     3841 tgttcggact tttgggacca aacggtgcgg ggaaatcaac aacaatttcc attatatcaa
     3901 cactcttgcc tcctacaaag ggcgaaatac tgtttgaagg agaaagtatt ttcaagaact
     3961 caaaaaattt aaggcagaaa ttaggaatag ttccccagga tgttgccctt tatcccaccc
     4021 ttactggata cgaaaattta aggttctggg ggaatttata cgggcttaag ggtgctgacc
     4081 ttaaaaagag aataaatgaa gtggcagaaa ttataggatt aaacggcaga ctaaaggata
     4141 aggtcgataa gtattccgga ggaatgaaaa gaaggcttaa cataggggcg gcacttcttc
     4201 atgaacctga actccttata atggatgagc ccactgtagg aattgacccc cagtccaggt
     4261 cccacatcct ggatacggtg ctgaaattaa atgctcaagg aatgacaata atatatacca
     4321 ctcactacat ggaggaggct gaagaactgt gcagccgaat ctgtataatg gatgaaggaa
     4381 agattattgc atcgggaaca aagcaggaat tggtggaatt ggtaaaggaa aaaactc
//
LOCUS       DAOPUT010000111         4415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_19924_length_4415_cov_19.943349, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000111 DAOPUT010000000
VERSION     DAOPUT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19924_length_4415_cov_19.943349"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..614
                     /gene="mraZ"
                     /locus_tag="PKK75_07680"
     CDS             177..614
                     /gene="mraZ"
                     /locus_tag="PKK75_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNZ83518.1"
                     /translation="MFTGEYIHSFDEKGRVIIPSKFRNELGESFYIGKGLDKCLFVYP
                     IETWTEFVNKLKNLSTFNKEERFFLRRFVSGFSECSFDKQGRILIPPQLRSFSELNTD
                     AAIIGVIDRIEIWNKNSWDDYSNNDQFDFDSIAEKMSELGIGL"
     gene            625..1554
                     /gene="rsmH"
                     /locus_tag="PKK75_07685"
     CDS             625..1554
                     /gene="rsmH"
                     /locus_tag="PKK75_07685"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNZ83519.1"
                     /translation="MGYVHKPVLLDESIDGLNIKEDGIYVDCTLGGGGHTQEILKRAA
                     KGKVIGIDQDDYAIEKAAEKLSAYKNFIPVRNNFSNIDEILNELDIQKIDGIIFDLGV
                     SSFQLDIPERGFSYNYDMPLDMRMDKSQTTTARHIVNGYEENELSRILWDYGEEKWAS
                     RIAKFIVQEREQEFIETTGQLVSIIKKAIPKQVRQEGSHPAKKTFQAIRIEVNRELEI
                     LETTIKSAVERLNVKGRICVITFHSLEDRIIKNTYADLNKDCICPPEFPKCMCNHRRM
                     LKIITKKPITPSEEELSENRRSHSAKLRIGEKV"
     gene            1558..2022
                     /locus_tag="PKK75_07690"
     CDS             1558..2022
                     /locus_tag="PKK75_07690"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNZ83520.1"
                     /translation="MSALRKEAVELQEHGREKEYSQKKRIKDKKRNNTFIESLKNSAM
                     IAITFILGILIVYNYALITDKQMELNNLGIEITNLNNEIDQYNIALESIKNTNSIEEM
                     AKVYLGMNYPTRKQTVFVDFTYGSDTTEEDNIMARDENLLVGLIDKVRSFIR"
     gene            2036..4153
                     /locus_tag="PKK75_07695"
     CDS             2036..4153
                     /locus_tag="PKK75_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ83521.1"
                     /translation="MKKRAKSTTNLQNKRRMLFFLVCFFLVTMALVVRLGYIQLIKGE
                     EYRKQAMENWSRDITINAKRGTIYDAKGKKLAVSINSNTVTCFPADVKKSVKPAVDNT
                     ESEDDSLLTAILKKLNKTMFTEETQADQIPVNTKTPEDIAEILSGILEMDYEEVFNKI
                     TSNYNYVVLKKWITDEQAQKIREQELSGISIIEDNKRVYPYGNFAPYVLGFTNIDQDG
                     LYGVEATYNDYLTGIPGRTVVNTDAYGRELPFGYNEYYESQDGSGVVLTIDEVIQHYA
                     ESAAEKVMADYNAIRATIIVMDPNTGDVLAMTSKPDYDPNDPRIPVDANVQAQWEALS
                     NEELQNAWFDMWRNPAVNDVYEPGSTFKLITTATALEENAATLNSTYFCDGYVRQITS
                     HNIKCWRYYRPHGQQTLSEAVQNSCNDALAQIGLDIGKEPFYKYLRSFGFEEQSGIML
                     NGEALGIVKKPEFMKDVDVVTQAFGHGVAITPIQLVNAVSAISNGGNLMVPRIVKQLI
                     DEDGNIIKNFDTEVRRKVISEETSKIMLRIMKDSAEAGTKQAYRPGYRIGGKSGTAQK
                     VIGGKYVEGKFISSFIGVAPVDKPRAVALVMVDEPDRSIGYYGSIVAGPVAADLLENT
                     LKYYDVEPVYTEEELGHVEKQMVTVPNLLGLTIADATDLLIKSGLESNITIEVEAERT
                     VKSQFPKAGEQVVKSSMITLVLN"
BASE COUNT         1592 a          728 c          962 g         1133 t
ORIGIN      
        1 gctccttgcg tcgcataaat tgggaattcg ttgcggttga cctaccaacc atttgtcaga
       61 aaaaacactc tgataaatgg ggttaggtca tacaaaagat tttttgcatt tttttaaaat
      121 aattgtggca ttttataccc actatgtggt ataatgcacc cagtaggtgg tagaaaatgt
      181 ttactggtga gtacatacat tcatttgacg aaaaaggaag ggtaattata ccttcaaaat
      241 ttagaaatga attgggtgaa agtttttata tcggtaaagg actggataaa tgcttgtttg
      301 tttatcctat tgaaacatgg acggaattcg ttaataagct taaaaattta tcaaccttta
      361 acaaggagga aagatttttc ttaagacggt ttgtttcagg tttttctgaa tgttctttcg
      421 ataaacaggg aagaatattg ataccgcctc agctgagaag cttcagcgaa ttaaatactg
      481 atgctgcaat tataggggtt atagacagaa tcgaaatttg gaataagaac tcttgggatg
      541 actactccaa caatgaccag ttcgattttg acagcatagc agaaaaaatg tcggaattgg
      601 ggataggctt ataaggagga aatcatggga tatgtgcata aaccggtttt gcttgacgaa
      661 tctatagatg gtctgaatat aaaggaagac ggtatttatg ttgattgtac tcttggcggg
      721 ggaggccata cacaggaaat attaaaaaga gcggctaagg gcaaagtcat aggaatcgac
      781 caggatgact atgcgataga aaaggcggca gagaaattat cagcttataa aaattttata
      841 ccggtaagaa ataatttcag caatatagac gaaattttaa acgaacttga tattcaaaaa
      901 atcgacggta taatatttga ccttggtgtt tcatcatttc agcttgacat accggagagg
      961 ggattttctt acaattacga tatgcccctt gacatgagga tggataaaag tcagacaacc
     1021 actgcaagac acatcgtaaa cggatatgag gaaaatgaat tgtccagaat tctttgggat
     1081 tacggcgaag aaaaatgggc gtcaagaatt gctaaattta ttgtacagga aagagagcag
     1141 gaatttatag aaacaacggg ccagcttgtt tctattatta aaaaggctat accgaagcag
     1201 gtcagacagg aaggttccca tcctgcaaaa aaaacatttc aggcaataag aattgaagtg
     1261 aacagagagc tggaaatact tgagaccaca attaagtccg cagttgaaag gctgaacgtg
     1321 aaagggagaa tctgcgtcat aacatttcat tcccttgagg acagaattat taaaaataca
     1381 tatgcagatt taaacaagga ttgcatatgt ccgccggagt ttcccaaatg catgtgcaac
     1441 cacagaagaa tgcttaaaat aataacaaaa aaaccaataa ctccatcaga agaagaattg
     1501 tcagaaaaca gaagatcaca cagcgcgaaa ttaagaatag gcgagaaggt gtaaaaaatg
     1561 tcagcgttaa gaaaggaagc tgttgagtta caagagcatg gaagagaaaa agaatattct
     1621 caaaaaaagc gaataaaaga caaaaaaaga aataatactt ttatagagag tttgaaaaat
     1681 tcagccatga ttgcaattac ttttatatta ggaatactaa tagtttataa ttatgccctg
     1741 atcactgaca agcaaatgga gctgaataat cttggtatcg aaataacaaa tctcaacaat
     1801 gaaattgacc agtacaacat agctttggag agcattaaaa acactaacag cattgaggaa
     1861 atggctaaag tatatttagg aatgaattat cctacaagga agcagacggt atttgtagac
     1921 tttacatatg gttctgatac aaccgaagaa gataatataa tggcaaggga tgaaaatctg
     1981 ttagtaggat tgattgacaa ggtaagaagt ttcattcgat aagggggatt gccatatgaa
     2041 aaagcgtgca aaaagcacta ctaaccttca gaataaaaga aggatgttgt tttttttagt
     2101 atgctttttt ttagttacca tggctttggt tgtgaggctt ggttacatac agctgataaa
     2161 aggtgaagaa tacagaaaac aggcaatgga aaactggtca agggatatta ccataaatgc
     2221 caaaagagga acaatttatg acgccaaagg gaaaaaactg gcagtaagca taaattcaaa
     2281 tactgtcacg tgtttcccgg cagatgtgaa aaaaagcgta aaacctgctg ttgacaatac
     2341 agaaagtgaa gatgacagcc ttttgacagc aattttaaaa aagcttaaca aaacaatgtt
     2401 tacagaagaa acccaggctg accaaatacc tgtaaatacc aagacaccgg aagatattgc
     2461 agagatttta tcgggaattt tggaaatgga ttacgaagag gtttttaata aaataacttc
     2521 caactataat tatgtagtcc taaaaaaatg gattaccgat gaacaggcac aaaaaataag
     2581 agagcaggaa ttaagcggca taagtatcat cgaagacaat aaaagagtat atccttacgg
     2641 gaatttcgct ccttatgtat tggggttcac aaatattgac caggacggtt tatacggagt
     2701 ggaagcaacc tataatgatt atctcaccgg aattcccggg agaactgttg ttaatacgga
     2761 tgcttacgga agggagctgc catttggata caatgaatat tatgaatctc aggatggctc
     2821 gggtgttgta ttaaccattg atgaggtaat acagcattat gcagaatcgg cagctgagaa
     2881 ggttatggcg gattacaatg caatacgggc gacaatcata gtgatggacc caaatacagg
     2941 cgatgtttta gcaatgacat caaagcctga ttatgatccc aatgacccga gaattcccgt
     3001 agatgcaaat gttcaagccc agtgggaagc cttgtccaat gaagagcttc agaatgcatg
     3061 gtttgacatg tggcggaatc ctgcagttaa tgatgtatat gagcccggct ccacatttaa
     3121 gcttataacc acggcaactg ccctggaaga aaatgctgca acacttaact ccacatactt
     3181 ttgcgacggt tatgtaagac aaattacaag tcacaacatt aaatgctgga gatactacag
     3241 acctcacgga cagcagacat tatcagaggc agtacagaac tcatgcaacg atgctcttgc
     3301 ccagataggt cttgatatag gaaaagaacc attttataaa tatttacgat ccttcggctt
     3361 tgaagaacaa tcaggaatca tgctcaatgg agaggcattg ggcattgtaa aaaaacctga
     3421 atttatgaag gacgtggatg ttgtaaccca ggctttcggc catggtgttg caataactcc
     3481 catacaactg gtaaatgctg tttccgcaat ttcaaacggc gggaatttaa tggtgccgag
     3541 aattgtgaaa cagctcattg acgaagatgg aaatataatt aaaaattttg atacagaagt
     3601 aagaagaaaa gtaatttctg aagaaacatc aaaaattatg ctccggatta tgaaggattc
     3661 cgcagaagca ggaacaaagc aggcttacag gccgggttac agaataggcg gaaaatcagg
     3721 aacagcacaa aaggttatcg gcggaaaata tgttgaaggc aaatttatat cttcatttat
     3781 aggagtagct ccggtagaca aaccaagagc agtggcactt gtcatggttg atgaacctga
     3841 cagaagcatc ggctattacg gctctatagt tgcagggccc gttgctgctg atttactgga
     3901 aaacacactt aagtattacg atgtagagcc ggtttatacg gaagaagaac taggtcatgt
     3961 ggaaaaacaa atggtgactg ttccaaatct attagggctt acaattgcag atgcaaccga
     4021 ccttttgatt aaatcaggtt tggaaagcaa cataacaatt gaagtggaag ctgaaagaac
     4081 agttaaatcc cagttcccca aagcaggaga gcaggttgtc aaatcctcaa tgattaccct
     4141 ggtactaaac taagtatgac ctaacccaat ttcttcgact gcaaggagaa agtcttgatg
     4201 gcaggtcaac agcacagaat gccaaattta tacgaccaat ggagtaaata aagagatgca
     4261 ttccactggg aaaataagaa aaatatccgc tctcggtttc ggccacgaat aattctaaag
     4321 ctcatgaccg ctaaaaatcc tacagcttgc aaactcgctg cgctcaaaca gtgcaagctg
     4381 cttaacggat ttttaacctt cattcgctaa gaatt
//
LOCUS       DAOPUT010000112         4355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_20294_length_4355_cov_20.067442, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000112 DAOPUT010000000
VERSION     DAOPUT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20294_length_4355_cov_20.067442"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..3063)
                     /locus_tag="PKK75_07700"
     CDS             complement(358..3063)
                     /locus_tag="PKK75_07700"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HNZ83522.1"
                     /translation="MEWYNKSKEEAISELNTDESSGLSEQEAALRLDKYGKNELAAQP
                     KKSLAAKLLAQFADFLIIILIVAAIVSAMVGEVSDSIVILAIVVINALLGIYQEGKAE
                     KSVEALQKMSAPNAKVLREGRQMEVPAANVVPGDIVILETGDIIPADVRLIESSNLKI
                     EEAALTGESVPVEKDAKEIIEGSAGIGDRHNMGFSSTIISYGRGKGLVFGTGHDTEIG
                     KIATTIQSYDEEQTPLQIKLNQLGKVLGTACIVICIIVFAVGLFQGRGVLEMLLTSIS
                     LAVAAIPEGLPAIVTIVLAIGMNRMAARNAIVKKLLAVETLGATTVICSDKTGTLTQN
                     EMTVVKAYVDDKILDVEGGGYEPHGAVKLDGEIINIDSLPDLRIFTSAGALANDALLD
                     NSSGLWRIAGDPTEGAIVTFAGKLGRKKDDLNKEFPRIEELPFDSARKMMSTFHDNYI
                     PGKIVSFTKGAPDIVIKRCTKIALNGDTVDFTEELKNRVLSVNTRFAKSALRVLSAAY
                     KVWDGLPEKNISEAVESDMIFLGLVGMIDPARPEVKDAIKECKDAGIEAVMITGDYKE
                     TAYAIAEQLGMADSEDQAMMGAELDNLSEDEIREVVKKTKVYARVSPQHKVAIVTALK
                     ENGNITAMTGDGVNDAMALKKADIGVAMGITGTDVAKNTAEVILTDDNFATIVNAVEE
                     GRIIFSNIKKFVFFLLSCNIGEILIVFTSILLGWEVPLLPIQLLWLNLVTDSFPALAL
                     GVEPAEPNIMNQKPRNTKEGILDKAMYGGIVFQAVAISGSCLLAYYLGAQKYGITDGL
                     PRVRTIVFTTLITAELLRAFSSRSQDYTLFKIGFFSNKAMNKAVFVSFALTVAVLYIP
                     IFIGIFRTVPLSTADWIYVLSLAFIPLITGEIYKKIFKKSISTVS"
     gene            complement(3330..>4355)
                     /locus_tag="PKK75_07705"
     CDS             complement(3330..>4355)
                     /locus_tag="PKK75_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ83523.1"
                     /translation="GRRRGAGLGGGNDEREQTLNQLLVEMDGFSGNEGIIIIAATNRP
                     DILDPALLRPGRFDRTVHVGLPDVKAREEILKIHVKKKPLAENIDLEIVAKRTPGFTP
                     ADLENAMNEAALLAARHNQTVITMDTIEEAITKVIAGPEKRSKVINENEKKLTAYHEA
                     GHAVVARLSSTKDPVHMITIIPRGGAGGFTMYLPEEDKSYRSKSEMEEYIVRLLGGRV
                     AEKLALDDISTGASNDIERATKIARAMVTTYGMSDNLGPMSYGTSDDEVFLGRDFNKI
                     RNYSEDIAAKIDNEMRRIIDNAYNKTEKLLSENIEKLHNVAQALLEKETITGKEFEIL
                     FDGGSIS"
BASE COUNT         1099 a          999 c          719 g         1538 t
ORIGIN      
        1 atttttgcaa tactttgggg ttaaatgatt cctattaatt caatcttgca agattttaaa
       61 aatgacatat tcccaataat aaatcaggga agctattttc atagttttcc ccttatcaaa
      121 ccttacatgc cctaataagg aatatggctt aatcaatatt ctcaaccctt cccatgattg
      181 gagcaaaggt accaaggtta cgtttatacc ggttaaaatt gcaattagtt attacaccgc
      241 atatatttgg acttattgta tattccaata taaaactatg cctagtacct ttgttggatt
      301 cattttgtgt cctgtaattc cgcctattgc cgaaagaagc agcatggagg cgaatactta
      361 cgatacagta cttatacttt tcttaaatat ctttttatat atttctcccg taatcaaagg
      421 aataaatgct aaagataaca catatatcca gtctgccgtt gataggggta ctgtacgaaa
      481 tatgcctata aatattggaa tgtacaaaac tgcaaccgtt aaagcaaatg atacaaagac
      541 tgccttattc attgctttat tcgagaaaaa cccaatctta aacaaagtgt agtcctgtga
      601 ccttgatgag aacgctctta aaagttctgc tgtaatcaag gttgtgaata caattgttct
      661 tactcttggc aaaccgtccg taattccata cttctgagca ccaaggtaat atgccagtaa
      721 acatgatcct gatattgcaa ctgcctggaa gacgattccc ccatacattg ctttatcaag
      781 gatgccttcc tttgtattcc ttggtttttg attcatgata ttgggctctg caggttcaac
      841 tccaagcgca agtgccggga agctgtccgt aacaaggttc agccataaga gttgaatcgg
      901 aagcaagggt acttcccacc ccaataatat gcttgtgaat actatgagga tttcacctat
      961 attacagctt aagaggaaga agacgaactt tttaatgtta gaaaatataa ttcttccttc
     1021 ttctacagca tttactattg ttgcaaagtt atcatccgtc aatattacct ctgcagtgtt
     1081 tttagcaacg tctgttccgg ttatgcccat tgctactccg atatcagcct tcttaagtgc
     1141 cattgcatca tttacgccgt ctcccgtcat agccgtgatg tttccgtttt ctttcaatgc
     1201 tgtcactatt gctaccttat gctgaggtga tacacgggca tatacttttg ttttctttac
     1261 aacttctctg atttcatctt cggaaagatt gtccagctct gctcccatca ttgcctggtc
     1321 ttcagaatct gccatcccta attgctcggc aattgcatat gctgtttcct tgtagtcccc
     1381 cgtaatcatt actgcttcaa ttccggcatc cttgcactct ttgattgcgt ccttgacttc
     1441 cggtcttgcc ggatctatca tgcccacaag tcccagaaat atcatgtcgc tttcaactgc
     1501 ttctgatatg ttcttctccg gtagtccatc ccataccttg tatgctgccg acaagactct
     1561 taaagctgat tttgcgaacc gggtgtttac cgataatact ctgtttttca gctcttctgt
     1621 aaaatcaact gtatctccgt ttaatgcaat ttttgtacat ctttttatta ctatatccgg
     1681 ggcgcctttt gtaaatgata caattttacc gggtatatag ttatcgtgga atgtgctcat
     1741 catttttcta gcagagtcaa agggcagttc ttcaattcgc gggaattctt tgtttaaatc
     1801 atctttcttc ctgcccagct tgcctgcaaa tgtaacaata gctccttccg tagggtcccc
     1861 ggctattctc cataatccgg atgaattgtc caggagagca tcatttgcca gagctcctgc
     1921 tgatgtaaat atccttaaat ccggaagtga gtctatgttt attatttctc catctaattt
     1981 aacggcacca tgaggttcat atccccctcc ttcaacatcc agtattttat cgtcaacata
     2041 tgcttttaca acagtcattt cattttgagt caatgtacct gttttatctg agcatattac
     2101 tgtggttgct cccaatgttt caactgccag gagttttttt actatggcat ttcttgcagc
     2161 catcctgttc atacctattg ccaaaactat tgtaacaatt gccggcagac cttctggtat
     2221 agcggcaact gccaaactga tggaagtaag gagcatttcc aaaactcctc ttccctggaa
     2281 aagacccact gcaaaaacaa ttatacaaat tacaatgcat gccgttccaa gcacttttcc
     2341 caattgattc aattttatct gcagcggtgt ctgctcttca tcatatgact ggatagtagt
     2401 tgctatcttt cctatttccg tatcgtgtcc tgtcccgaaa actaatccct ttcctcttcc
     2461 gtatgaaatt atagtgcttg aaaatcccat attatgcctg tcgcctatac ctgctgagcc
     2521 ttcaatgatt tcttttgcat ccttctcaac aggtacggat tctccggtta atgctgcttc
     2581 ttcaatctta agattcgagc tttctataag tcttacatct gctggtataa tatcccccgt
     2641 ctctaaaata actatatcgc cgggcaccac attggctgcc ggcacttcca tctgtctgcc
     2701 ttctcttaaa accttggcat tgggtgcgct cattttttgc agtgcctcta ctgatttttc
     2761 cgccttacct tcctgataaa tacccagtaa tgcgttaatt acaacaattg ctaaaattac
     2821 aattgaatct gatacttctc ctaccattgc tgaaactatt gcagctacaa tcagaataat
     2881 aattaaaaag tcagcaaact gggctaacag ttttgcagcg agactttttt tcggttgggc
     2941 tgccagttcg tttttaccat acttatcaag tcttagggct gcttcctgtt cactcagtcc
     3001 agaactctca tcggtattaa gctcagaaat tgcttcttct ttacttttat tgtaccattc
     3061 catattcgta ccttctcttt tgtatttcat ttatttgttt tcaacttgtc agattgaggg
     3121 atgcaaatta ccatggccat cttaaccgcc tgcaagatat tactgcagct taatcttttt
     3181 gctatatatt aatacgaatt taatattttt gcaatacttt gggaataaat gattctgatt
     3241 aatttaatct tgcaaaaatg gtgtaaggcc atgtttatca ataaaacaag gaacggctgt
     3301 gccgttccat atttgtcctt tgaaaatctt tagcttatac ttcctccgtc gaagagtatt
     3361 tcaaactctt tgccggttat tgtttccttt tcaagaagag cctgagccac attgtggagt
     3421 ttttcaatat tttcgctcaa gagcttttca gttttgttat aagcattatc tatgattctt
     3481 cgcatttcat tgtcaatttt tgctgctata tcttcactgt aatttctgat tttattgaag
     3541 tctcttccca ggaatacttc gtcatcgctt gttccataag acatgggtcc taagttatcg
     3601 ctcatgccgt atgttgtaac cattgctctg gcaatttttg ttgctctttc aatatcgttt
     3661 gaagcacctg ttgatatatc gtcaagggct aatttctcag caaccctgcc ccccaataat
     3721 cttacaatat actcttccat ttcagatttt gacctgtatg atttgtcttc ttcaggaaga
     3781 tacatggtaa atcctcccgc tccgcctctg gggatgattg taatcatgtg aaccgggtcc
     3841 ttggttgatg atagtcttgc tacaaccgca tgacctgctt catgataagc agtgagtttc
     3901 ttttcgtttt cgttaataac cttgcttctc ttttctggtc ctgcaataac ctttgttatt
     3961 gcttcttcaa tagtatccat tgtaataacc gtctggttat gtcttgcagc taaaagtgcc
     4021 gcttcattca ttgcattttc caaatcagcc ggcgtaaacc cgggagttct ttttgcgact
     4081 atttccaaat ctatattctc tgccaaaggt tttttcttta catggatttt caatatttct
     4141 tctcttgcct ttacatcagg aagtcctaca tgaaccgttc tgtcaaatct tcccggtctt
     4201 aacaatgcag ggtccaaaat atcaggtctg tttgttgctg caatgataat tataccttca
     4261 ttgcctgaga atccgtccat ttcaaccagc agctggttca atgtctgctc tctttcatca
     4321 ttgcctcccc cgagacctgc acctcttctt cttcc
//
LOCUS       DAOPUT010000113         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_20353_length_4344_cov_22.440196, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000113 DAOPUT010000000
VERSION     DAOPUT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20353_length_4344_cov_22.440196"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="rlmD"
                     /locus_tag="PKK75_07710"
     CDS             complement(<1..1136)
                     /gene="rlmD"
                     /locus_tag="PKK75_07710"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNZ83524.1"
                     /translation="METKNNYINDISDCPVMDKCGGCQLQNYSYEKQLQEKQKQVNML
                     LDKYCRVETIIGMKNPYYYRNKVHAVFDTDKKGNIISGVYEAGSHRVVKVDSCLIEDQ
                     RADKIIVTIRGMLKSFKIRTYNEDAREGLLRHVLIRTGHKSGEVMVVLVLASHIFPSK
                     NNFIKVLLKAHPEITTIVMNVNNKKTSMVIGEREQVLYGKGYIEDTLCGKVFRISPKS
                     FYQINSTQTEILYGKAIELASLKGNESIIDAYCGIGTIGIIASDQVRKVTGVELNQDA
                     IKNAITNAKRNKVKNIYFFNQDASEFMSQMAHEKQTADVVFLDPPRTGSTEQFLGSMV
                     SMAPKKVIYISCNPVTLERDISYMVKRGYKAETAIPVDMFPGTE"
     gene            complement(1175..>2378)
                     /gene="rlmD"
                     /locus_tag="PKK75_07715"
     CDS             complement(1175..>2378)
                     /gene="rlmD"
                     /locus_tag="PKK75_07715"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNZ83525.1"
                     /translation="STEAKLLEVLKNASYEQEPSCEHFNLCGGCTWQTVPYEKQLEIK
                     ATQVKTILNEVLTENYEFLPIVPSPKKSEYRNKMEFSFGDETKGGPLTLGMHKRNSYH
                     SIVDTQGCQIVDRDFRQILCRTIDYFRGNSQTFYQKTTNIGFLRYLLVRKAEKTGEIL
                     INLITTSQGTLNEKEFVELILNTNLQGTVKSISHSIFDGVADAAKADTMKILYGEDKI
                     TEELLGLKFNISSFSFFQTNSLAAEKLYSIVRDFIGDTKDKVIFDLYSGTGTIGQILA
                     PAAKKVTGIEIVEEAVKKANENAKLNNINNCTFIAGDVLKKIDELNEKPDIIVLDPPR
                     AGIHPKAIQKIIDYKPETFIYISCKPTSLKNDLPGFLENGYRITKVQCIDMFPQTPNV
                     ETVLLIEK"
     assembly_gap    2379..2478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2479..4221)
                     /gene="ptsP"
                     /locus_tag="PKK75_07720"
     CDS             complement(<2479..4221)
                     /gene="ptsP"
                     /locus_tag="PKK75_07720"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNZ83526.1"
                     /translation="MKGLGVSPGIGIGKAFIIDKESVNITRHYVNDVENELDRLKAAI
                     ETAKEQLNELYISAIDELGEKEAQIFKSHEMMLEDTTFMSDVENRIIDEGINAEFALY
                     ETSNVYIKMFQNIENEYLRERAEDIKDVMGRVIRLLMGITSFDFSNLEENSIIVAKDL
                     TPSDTAQIDRTKVAAMITEMGGKTSHAAIIARILGIPTVVGLDNITDKIKSGDTVICE
                     GKTGKVIVNPNEKQLFHYNRKKIYEDEIKHELRNQIGLPSVTKDGFGVSLSANIGTPN
                     DVDMVLENDAEGIGLFRSEFIFMNRDHQPTEDEQFEQYKEVLVKMGDKPVIIRTLDIG
                     GDKNVPYIDIPKEMNPFLGYRAIRLCLGNVEVFRTQLRAILRASVYGNVKIMVPMIST
                     MKELKDSKKILQQAKDELTREGIKFKDDIEIGIMIEIPSAAIISDLLAREVDFFSIGT
                     NDLIQYTMAVDRMNSKLSYLYSQYHPALLRLIKGIIDNAHNAGIQVGMCGEAAGDPKL
                     IPVFIGMGLDEFSMNAPSILRSRYIVRNANKKDMEIIARNTLDMENALEVEEYLSCLF
                     DENENCLKSCKNRRS"
BASE COUNT         1057 a          870 c          699 g         1618 t
ORIGIN      
        1 tgttctgttc ccggaaacat atcaacaggt atggctgttt cagctttgta gccccttttc
       61 accatgtatg atatgtctct ctcaagagta accgggttgc atgaaatgta aatcaccttt
      121 tttggagcca ttgataccat ggatcccaag aattgttcgg tgcttccggt ccttgggggg
      181 tctaaaaaga ccacatctgc ggtttgtttt tcatgtgcca tctgagacat aaactcactt
      241 gcatcttgat taaagaaata tatatttttc actttgttcc tttttgcgtt ggttattgca
      301 ttttttattg catcctggtt tagctctacc cctgtaacct ttcttacctg gtcgcttgca
      361 attattccta tggttcctat gccgcagtat gcgtcaatga tgctttcgtt accttttaat
      421 gatgccaatt ctatagcttt tccgtagagg atttctgttt gtgtggagtt tatctgataa
      481 aacgatttcg gagaaattct aaaaacttta ccgcacaaag tatcttctat atatcctttc
      541 ccatacagca cctgttctct ttctccaata accatactgg tttttttatt attcacattc
      601 attactatgg ttgttatttc aggatgagcc ttcagtaata ccttaatgaa attgttttta
      661 gatggaaata tatgtgatgc caaaaccagg acaaccataa cttccccgct tttgtgccct
      721 gttcttatta atacatgccg taaaagacct tctcttgcat cttcattgta ggtccttatt
      781 ttaaatgatt ttagcattcc cctgattgta actattattt tatcggcccg ctggtcttct
      841 attaaacagc tgtcaacttt gaccactctg tgggaccctg cttcatacac tcctgaaata
      901 atgtttcctt ttttatccgt gtcaaatact gcatgaactt tgttgcgata ataatacggg
      961 tttttcattc ctataattgt ttctaccctg caatatttat ccaaaagcat attaacctgc
     1021 ttttgctttt cctgtagttg tttttcatag ctgtaattct gcaactggca gcccccgcat
     1081 ttatccatga ctggacaatc actaatatcg ttaatatagt tattttttgt ttccattaga
     1141 actcctgtat ataagaatac ttttaatcaa cctattactt ctctatcaac aaaactgtct
     1201 caacgttggg tgtttggggg aacatgtcta tgcattgaac ctttgttatc ctgtatccat
     1261 tttctaaaaa acccggcagg tcatttttta gggatgttgg tttgcatgaa atgtaaatga
     1321 aggtttcggg tttataatcg attatttttt gaattgcttt tggatgaatg cctgcgcggg
     1381 gcgggtccag gactatgatg tcaggttttt cgtttaattc gtctattttt ttgagaacat
     1441 ctcccgctat gaaggtacag ttgtttatat tgttaagttt tgcattttca ttggcttttt
     1501 ttactgcttc ttcaactatt tctatgcctg ttactttttt agctgcagga gctagtattt
     1561 gtcctatggt tcctgttccg gaatacaggt caaatattac tttgtctttt gtgtctccaa
     1621 taaaatctct tacgattgag tatagttttt ctgctgccaa ggaatttgtc tggaaaaagg
     1681 aaaaagaaga aatgttgaat ttgagaccta acagctcttc agttatctta tcttccccgt
     1741 agagaatttt catagtatca gcctttgctg catctgcaac tccgtcaaat attgagtgag
     1801 agatggattt taccgttcct tgcaggtttg tgtttagaat aagctcgaca aattcctttt
     1861 catttaatgt gccttggctt gttgtaatta aattaattag aatttctcct gttttttctg
     1921 cctttctcac taataagtat cttaaaaaac caatatttgt ggttttctga tagaaggttt
     1981 gactatttcc gcggaaataa tctattgttc gacataatat ttgtcgaaaa tctctatcga
     2041 caatttgaca gccctgtgtg tcaactatgc tgtgatagct gtttctttta tgcattccca
     2101 gtgtcagggg accgcccttt gtttcgtcgc cgaatgaaaa ttccattttg tttctgtatt
     2161 cacttttctt agggcttggc actatgggca ggaattcata attttctgtc aatacttcat
     2221 ttagaattgt tttgacttgt gtggctttta tttcaagctg cttttcatag ggaaccgttt
     2281 gccaggtaca acctccgcat aggttgaaat gctcgcagga tggttcttgt tcgtaagatg
     2341 cattttttag cacttcgagg agctttgctt ctgtgctgnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnac ttctcctgtt tttgcatgat tttaggcaat tttcattttc
     2521 atcaaacaaa cagcttaaat actcctctac ttcaagtgcg ttttccatat caagtgtatt
     2581 tcttgctatt atctccatat cttttttatt tgcattcctg actatatatc ttgacctgag
     2641 gattgaaggg gcattcatgc tgaattcatc taatcccatt ccaatgaata ccggtataag
     2701 ctttggatcg ccggctgctt cgccgcacat gcccacctgg atgccggcat tgtgtgcatt
     2761 atcaattata ccctttatca gccttaaaag tgcaggatgg tattggctgt ataaataaga
     2821 tagtttggaa ttcattctgt caacagccat ggtatattga atcaaatcat ttgtacctat
     2881 gctgaaaaag tcgacctccc tggctaaaag gtccgatatt atagcagcag aaggaatttc
     2941 aatcattatg ccgatttcta tatcatcctt gaattttatt ccttcccttg ttaattcatc
     3001 ctttgcctgc tgcaggattt tctttgaatc ctttaattcc ttcatagtgg aaatcattgg
     3061 aaccattatt ttaacatttc cataaacgct ggctctcagg attgccctca gctgtgttct
     3121 aaaaacctct acattgccaa gacacagcct tattgccctg tatcccaaaa aaggattcat
     3181 ttcttttggt atgtcaatgt aaggaacgtt cttgtcccct cctatatcca gtgttcttat
     3241 tattacaggc ttatcaccca ttttaacaag aacttcctta tattgctcaa attgttcgtc
     3301 ttcagtgggc tgatggtctc tgttcataaa tatgaattcg cttctgaaaa gacctattcc
     3361 ttctgcatca ttttccaata ccatgtctac atcattgggg gttcctatat ttgctgacag
     3421 cgagactcca aatccatcct ttgttactga aggcagtccg atttgatttc tcaattcatg
     3481 tttaatttca tcttcataaa tctttttcct gttataatga aacagttgtt tttcattggg
     3541 attaacaata acctttccag tctttccttc gcagattaca gtatcgccgc tctttatttt
     3601 atcggttata ttatctaacc ccacaacagt ggggatgcct aatattctgg caattattgc
     3661 tgcgtgggaa gttttgcctc ccatctcggt aatcatggcc gctacctttg tcctgtctat
     3721 ttgtgcagtg tcagacggtg tcaaatcttt tgcaacgatt atagaatttt cttctaaatt
     3781 tgaaaaatca aaactcgtaa tacccatcag cagtcttatt actctgccca ttacgtcctt
     3841 gatgtcttcg gctctttccc ttaagtattc attttctata ttttgaaaca ttttaatata
     3901 gacattggag gtttcatata atgcaaactc tgcgtttatg ccttcgtcaa ttattctgtt
     3961 ttctacatct gacatgaatg tcgtatcttc cagcatcatt tcatgagatt taaaaatttg
     4021 agcttctttt tcacctaatt catcaattgc agaaatgtat aactcgttaa gctgctcctt
     4081 tgccgtttct atagcagcct taagcctgtc taattcattt tcaacgtcgt ttacataatg
     4141 tctagttatg ttaactgatt ccttgtcaat tataaaagct ttacctatac caatacccgg
     4201 tgacactccc aaacctttca taacttcact acctttcagc tatttaagtt gttattatat
     4261 atgattaatc ctcttaaaga cattagatgc gcattccatg taaatttaaa atcttccaga
     4321 ttcattaatt tatagttata tagt
//
LOCUS       DAOPUT010000114         4343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_20358_length_4343_cov_19.848181, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000114 DAOPUT010000000
VERSION     DAOPUT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20358_length_4343_cov_19.848181"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1196)
                     /locus_tag="PKK75_07725"
     CDS             complement(6..1196)
                     /locus_tag="PKK75_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ83527.1"
                     /translation="MNYRTNQKNGDELSILGFGCMRLPVKAGIIDEARAIKMIRDSIE
                     KGVNYFDTAYIYHGGQSESLLGKALQDGYRPRVKIATKLPPYMVKKLNNAKKIMLTQL
                     EKLHTDYIDYYLLHMLTDKAMYDKMASLGVMEWLEELKAKGTIRNIGFSFHGSRRDFE
                     MIVKAYPWDFCQIQYNYLDENNQATVSGLKLAHSLGIPVIVMEPLRGGKLVNNLPKEV
                     LKTFKSFHPEWTPAEWGLRWIWNHKEVNVVLSGMSIEEQVNENIRIASDAASDSLSED
                     ELKVFGEVKSIMNEKIKVPCTGCGYCMPCPCGVDIPGCFSNYNDKFLLGEKLNRWKYA
                     QTLGVLSEKPAFASKCIECGKCEPHCPQNIEIRKELKSVARVMEGPLFRPIVGTARKI
                     LKIK"
     gene            complement(1193..1867)
                     /locus_tag="PKK75_07730"
     CDS             complement(1193..1867)
                     /locus_tag="PKK75_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ83528.1"
                     /translation="MLKIEDSNYIKENLDFWDKLSNSEKQLIENSFTPIKYSKGENLH
                     GAGRECLGLILVKSGELRAYILSEEGRDVTLYRLHEGESCILSASCILSNITFDVLID
                     AEQDTEVLLLNVGTFSKLSKENIYVENFMYKKSADRFSDVMWAMEQILFMSFDSRLAG
                     FLHDEIIKTKSNTIKLTHEQIAKYVGSAREVVSRMLKSFESQGILKLSRGTIEIIDKD
                     KLRKLI"
     gene            complement(1959..2696)
                     /locus_tag="PKK75_07735"
     CDS             complement(1959..2696)
                     /locus_tag="PKK75_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83529.1"
                     /translation="MLKKYVLKLLFRISLLVFVVYLYIFKRDLLSITGSYHIGGIRPL
                     HVIWIILMLEMIIQIRPTSKITMGCLKQFGDKYSEPSEPYNRLKLYENVQKMNISALK
                     VLLSWLLFNALIGIVYLSGMIGETELILIMMFYYVSDLICVIIWCPFQSFIMKNRCCV
                     NCRIFNWGHFMMYTPMLFVRSFFSWSLFFMSILVLIRWEITYLKYPERFWDGSNTAIR
                     CESCSDNLCYIKNGLKSRWKKEFKGSR"
     gene            2906..3388
                     /locus_tag="PKK75_07740"
     CDS             2906..3388
                     /locus_tag="PKK75_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNZ83530.1"
                     /translation="MKTNKLTALILILLFAVSPVAFAFGYFPSSGQGYEERVVQLVNE
                     ERAKAGLDALYIDSKISDVARAKSKDMADLNYFAHYSPTYGMANDMLLRFGITYSFWG
                     ENIAMGQDNPEIVVSEWMNSPGHRENILSPNFTFIGVGYYVDTDGTPYWTQIFTSDDK
                     "
     gene            complement(3459..>4343)
                     /locus_tag="PKK75_07745"
     CDS             complement(3459..>4343)
                     /locus_tag="PKK75_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83531.1"
                     /translation="KIELSERQFLTDFENKLQEYPKRKNMNFDNVMFITEARIEELNK
                     IIRLSNTKEEIIKAMNDRIEIDAKKHNDDIAALKSEMSERDGKIERLKEDILELRDEL
                     NKIHLQMMIKDSGHEDEINDLKSIIEWWKAKKLNHPCKSEDIPQWVEKEFAGKLIFHS
                     RAAGEIKKKPSFELDMNLLCDAIEYLANEYRDERLGLINKEKSNSICSEKYGRNFEVT
                     PAGDSSIEMYPKEYKVKYGKGPTGKSREVPLNHHLKVGIDNRNIIRIYFFYDKEEDLV
                     VVGSLPEHLKSVNKSKKR"
BASE COUNT         1253 a          926 c          692 g         1472 t
ORIGIN      
        1 acttatcatt taattttaag tattttcctt gcggttccca ctattggtct gaacaagggc
       61 ccttccatca cccttgcaac agactttagt tcttttctga tttcaatatt ctggggacag
      121 tgaggctcgc acttgccgca ttctatacac tttgacgcaa acgccggttt ttcagataat
      181 actcccagtg tctgggcata tttccaccta ttcagctttt cacccagtaa aaacttgtca
      241 ttatagtttg aaaagcagcc cggtatatca accccgcagg gacagggcat gcaatatccg
      301 catccggtgc agggcacctt gattttttcg ttcataatgg atttaacttc accaaaaacc
      361 ttcagctcat cttcagacaa ggagtccgaa gctgcatctg aagcaattct aatattttca
      421 tttacctgct cttcaatgct cattcccgac aaaaccacat ttacttcctt gtggttccaa
      481 atccatctga gcccccattc agcaggcgtc cattcaggat ggaagctttt aaaagttttc
      541 aaaacctctt taggcagatt attgactaat ttgcctcctc tcaggggctc cataacaatc
      601 accggaatcc ccagagaatg tgcaagcttg agccctgaaa cagttgcctg attattttca
      661 tccaaataat tatattgtat ctggcagaaa tcccatggat atgcttttac aatcatttca
      721 aaatcacgtc tgctcccatg aaaggaaaag ccgatgtttc ttatggttcc cttagctttc
      781 agttcttcca gccactccat aacgccaagg ctggccattt tatcatacat agccttatcc
      841 gtcagcatat gcagcaaata atagtcaata tagtctgtat gaagtttttc cagctgggta
      901 agcattattt tctttgcatt attcagtttc tttaccatgt atggcggaag cttcgtagca
      961 atttttacac gcgggcggta accgtcttga agtgcctttc ccagcaatga ttcgctttgg
     1021 cctccatgat aaatataggc ggtatcaaaa taattgactc ccttttcaat tgaatcgcgt
     1081 atcattttaa ttgctcttgc ttcatcgata attcctgcct taacaggaag cctcatgcag
     1141 ccaaaaccaa gaatggaaag ttcatccccg tttttttgat ttgttctgta gttcatatta
     1201 attttctcaa tttatccttg tcaattattt ctatcgttcc tcttgaaagt ttaagtatac
     1261 cctgggattc aaagctttta agcatccttg acacaacttc ccttgcgctg cccacgtatt
     1321 tggcaatctg ctcatgggtg agtttaatag tattagactt ggtcttaata atttcatcat
     1381 ggagaaatcc tgcaagcctg ctgtcaaaac tcataaataa gatttgctcc attgcccaca
     1441 ttacgtctga gaacctgtca gcagactttt tatacataaa gttttccaca tagatgtttt
     1501 ctttagacag cttgctgaag gttcccacat taagcaggag cacttccgta tcctgctcag
     1561 catctattaa aacatcaaat gttatattgc ttaaaatgca ggaagcagaa agaatacatg
     1621 attctccctc gtgtaagcgg taaagagtaa catctcttcc ttcctccgat aaaatatatg
     1681 ccctgagctc tccgctttta accagtataa gccctaagca ttccctgccc gctccgtgga
     1741 gattttctcc cttactgtac tttattggtg taaaactatt ttcaatcagc tgtttttcag
     1801 aattgcttaa cttatcccaa aaatccagat tttcctttat gtaatttgaa tcctctattt
     1861 tcagcattga gtccctcctc tgattttcta tattataaca tatttagctt aacataccca
     1921 ataaaaaaac gcatttaaac tttaaaggac cccctcattc accgggatcc cttgaattct
     1981 tttttccacc ttgatttcaa tccattttta atatagcata aattgtcact gcatgattcg
     2041 catctaatgg ctgtatttga tccatcccag aatctttcgg gatacttgag gtatgttatt
     2101 tcccaccgta tgagcactaa tatggacatg aaaaacaaag accatgagaa aaaacttctt
     2161 acaaataaca tgggtgtgta catcatgaaa tgcccccagt taaaaattct gcagtttaca
     2221 cagcacctgt ttttcattat aaaactttga aatgggcacc atataataac gcatataagg
     2281 tcactcacat aataaaacat cataatcaat ataagttcag tttcacctat cattccggac
     2341 aaatatacaa taccgatcag agcattgaac aagagccagg ataacaggac ttttaatgca
     2401 gaaatattca tcttttgaac attttcatat agtttaagcc tgttgtacgg ctctgaaggc
     2461 tcagaatatt tatccccgaa ctgcttcaaa caacccatgg ttattttgct tgtaggcctt
     2521 atctgtatta tcatttcaag catcagaatt atccatatga catggagggg tcttatgcct
     2581 cctatatgat atgaaccggt aatactaagg aggtctcttt taaaaatata caaataaact
     2641 acaaacacta acagtgaaat tcggaataac agctttaata catacttttt tagcatgttc
     2701 ccctcctctt gatgctaatt attataacat aaaattaaga aaatttcatc aaataattta
     2761 tcaaaagtcg cacggctgga taattggaaa acatatttaa tttttcaaaa aatatgctgc
     2821 caagcaaata tatgaaatta tatattagta tttaaatgaa ataatataat tgaaacaaat
     2881 ttttgtttca gaataaggag gttgtatgaa gacaaataag ttaacagctt tgattttgat
     2941 actgcttttt gcagtgtcgc ctgttgcatt tgctttcggc tattttcctt catcaggcca
     3001 gggttatgaa gaaagggttg tccagctggt gaatgaagaa agagcaaaag caggactgga
     3061 tgcactgtat atagacagta aaatttcaga tgttgccagg gcaaaatcaa aagatatggc
     3121 tgatttaaat tatttcgccc attattcacc aacttatggc atggctaatg acatgctgct
     3181 tagatttgga atcacttatt ctttctgggg tgaaaatata gccatgggac aggataaccc
     3241 tgagatagta gtttcagaat ggatgaattc tccaggtcac agagaaaata ttctttcacc
     3301 taattttaca tttataggtg taggatatta tgttgataca gatggaactc catattggac
     3361 acaaatattt accagtgatg acaaataagc aggctttcta aggtcaaaaa atacgttctt
     3421 ttctaaaaga acgtattttt ttatattcac cctaggggtt atcgcttttt gctcttattt
     3481 acagacttca aatgctccgg cagagagcct acaacaacca aatcctcttc tttgtcataa
     3541 aagaaatata ttctgattat attcctgttg tctataccta ccttcagatg atggttaagg
     3601 ggaacttccc ttgacttgcc tgttggacct ttgccgtatt taaccttgta ttcctttgga
     3661 tacatttcaa tactgctgtc accggcaggt gttacttcaa aattcctgcc atacttttct
     3721 gagcagatac tgttgctttt ctccttattg attaagccca acctttcatc ccggtattca
     3781 tttgcaagat attcaatagc atcacaaagc aggttcatgt ccagttcaaa ggaaggtttc
     3841 tttttaattt cgccggctgc ccggctgtga aaaataagtt ttcctgcaaa ctctttctcc
     3901 acccattggg gtatgtcctc agacttgcag ggatggttaa gctttttggc cttccaccat
     3961 tcaataattg acttcagatc attaatttca tcttcatggc cgctgtcttt tatcatcatc
     4021 tgcaaatgaa ttttattaag ctcatctctc agttctaaaa tatcttcttt taatctttcg
     4081 attttaccgt ctctttcact catctcgctc tttaaagcag caatatcatc attatgtttc
     4141 tttgcatcga tttctatcct gtcattcatt gctttaataa tttcttcttt tgtattgctt
     4201 aatctgataa ttttgttaag ctcttcaatc cgggcttcgg taataaacat tacattgtcg
     4261 aaattcatat ttttgcgttt cggatattcc tgcagcttat tctcaaaatc cgttaaaaat
     4321 tgtctttcac tcagttcaat ttt
//
LOCUS       DAOPUT010000115         4329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_20437_length_4329_cov_17.802995, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000115 DAOPUT010000000
VERSION     DAOPUT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20437_length_4329_cov_17.802995"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKK75_07750"
     CDS             complement(<1..290)
                     /locus_tag="PKK75_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HNZ83532.1"
                     /translation="MAKATREAYGDALKKLAANPDIVVLDADLSGSTKTSEFKKISPE
                     RFFNVGIAEQNLIGTAAGLSLAGKIPFASSFAMFAAGRAFEIVRNTVAYPAL"
     gene            complement(283..1131)
                     /locus_tag="PKK75_07755"
     CDS             complement(283..1131)
                     /locus_tag="PKK75_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNZ83533.1"
                     /translation="MKYDELKVIAKSMRKNILTMIYKAQSGHPGGSLSAVEIMTYLYY
                     REMNIDSPTDENRDRFVLSKGHGAPVLYAVLMEKGFIDKELLPTLRQVDSKLQGHPDM
                     KKLPEAGVEASTGSLGQGLSIANGMALAFKLDKKTNRVFVLLGDGEINEGMIWEAAML
                     ASHYKLDNLTAVLDHNGLQIDGRNEDVMTITPIDKKFEAFGWHVIKADGHDFNSLEKA
                     FEERKTIKGKPAIIIAETTKGKGCSFMENEAGWHGKAPNDQQFCKAMEEIGDIGDKRE
                     GGGLNG"
     gene            complement(1208..1852)
                     /gene="fsa"
                     /locus_tag="PKK75_07760"
     CDS             complement(1208..1852)
                     /gene="fsa"
                     /locus_tag="PKK75_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNZ83534.1"
                     /translation="MKLFIDTANVEEIRKANDMGVICGVTTNPSLIAKEGRIFKDVVR
                     EITSIVDGPISAEVISLDSEGMIREAVELAKIHKNIVIKIPMTVEGLKAVKVLSKENI
                     KTNVTLIFSAGQALLAAKAGASYVSPFVGRLDDIGNDGMTIIKEIVDIFWNYEIDTEI
                     IAASIRHPMHVIEAAKSGCHIATIPYKVIEQLAKHPLTDKGIDQFLKDWDTVPK"
     gene            complement(1937..2704)
                     /locus_tag="PKK75_07765"
     CDS             complement(1937..2704)
                     /locus_tag="PKK75_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNZ83535.1"
                     /translation="MDKLSIMGVRINNISMNEVLKAAEEKIENDEKYVIYTPNTEIIM
                     MCQKDEEFLDIMNRSDVNVPDGVGLIYAGKIKKLPLKEKVAGYDLSINLLKMADEKGL
                     KLYAVGGKPGIAEAAMKNVQKRYPGIKIVGAQHGYFKGTHLGNHGHQEERAVIEDINK
                     HKPHILFVGFGAKKQEQWIEYNKDLLNANVIIGNGGTLDGLAGIVKRAPDIFINLGLE
                     WFYRLIKEPKRITRQIVLPEFLLKVIFGNKDIVREIE"
     gene            complement(2697..3749)
                     /gene="csaB"
                     /locus_tag="PKK75_07770"
     CDS             complement(2697..3749)
                     /gene="csaB"
                     /locus_tag="PKK75_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HNZ83536.1"
                     /translation="MLLAGYYGFGNLGDEAILEMTLKQIFEITDRENITVLSGNKAIT
                     SKRYNVNTIDRYNVLSILNALKSADVLIFGGGSLLQDVTSKRSIYYYLFLIRLAKLMK
                     NKVVMLSQGIGPITCENSIKAVRSTLSLVDYITVRDKHSKEFLESIGVDEKKIFLSTD
                     PVINLRKDNSIEYEKQSTRKVCFSLRNWKGSDVSTKICKVAEKLIDDGIECHFIPFYH
                     KEDLQLIDQVEEILGSKAFYYRDRLSTGDAFDIIKTMDVMVGVRLHSLIFAAAANVPI
                     VAVSYDHKVDHFVNSVNMKIACRIDDIDENTIYDEIIYKINNEQEEKAMLSKSVDKIR
                     ELTNINYKILKEISNG"
     gene            complement(3755..>4329)
                     /locus_tag="PKK75_07775"
     CDS             complement(3755..>4329)
                     /locus_tag="PKK75_07775"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HNZ83537.1"
                     /translation="MDIFRGVKFAQILPFGIFIIMFIIYFMNKDEDSVKGVVNTTVRF
                     LNKGIKIYYGIIAGILALAGYIYISRTGHETEIQPSNIEMITRNFMEYMLLARPRTKE
                     FLIAFPAIFGAVFAAGKKSEFYTGIFMLAGAIGTSSVINTFSHIRTPIYLSFARTVIA
                     LVFGIVIGCVLVLVCSICNRAFVKIQERLK"
BASE COUNT         1126 a         1018 c          672 g         1513 t
ORIGIN      
        1 tttaaagccg gatatgccac tgtatttcta actatttcaa aggctcttcc tgcagcaaac
       61 attgcaaagg agcttgcaaa cggaattttc cctgccagag ataatcctgc agcagtgccg
      121 attaaattct gctcggcaat tcccacattg aaaaatctct cgggacttat ttttttaaat
      181 tctgaagttt ttgtagaccc ggacaggtcc gcatccaata caacaatatc aggatttgct
      241 gccagctttt ttaacgcatc gccgtatgct tctcttgttg ccttagccat tcaatccgcc
      301 cccttccctt ttgtctccta tgtctcctat ttcctccatt gccttgcaga attgctggtc
      361 atttggtgcc ttcccgtgcc agcctgcttc attctccata aacgaacagc ccttcccctt
      421 ggttgtttct gcaataatta ttgcgggctt tcctttgatt gtttttcttt cttcaaaggc
      481 tttttcaagg gaattaaaat catgaccgtc tgcttttatc acatgccatc cgaatgcttc
      541 aaatttcttg tcaatgggag taattgtcat aacatcttca tttctgccgt ctatctgcaa
      601 accgttatgg tcaagtacgg cagttagatt atcaagcttg tagtggcttg caagcatagc
      661 tgcttcccat atcattcctt cgtttatctc cccgtctccc aatagtacaa ataccctgtt
      721 tgtttttttg tcaagcttaa atgccagtgc catgccgttt gcaatggata gtccctgccc
      781 cagagaccct gttgatgctt caactccggc ttcaggaagt tttttcatgt ccggatggcc
      841 ttgaagcttg gaatcaacct gccttaatgt aggaagaagc tctttgtcta taaagccctt
      901 ttccatcaag acagcataaa gtaccggtgc cccgtgtcct ttggataata caaatctgtc
      961 cctgttttca tctgtgggag aatctatgtt catttccctg tagtataaat atgtcattat
     1021 ctccaccgca gaaagggagc ctcccggatg gcctgactgt gccttataaa tcatagtcag
     1081 tatgtttttt ctcatacttt ttgctatgac cttcaactca tcgtatttca tatttcctcc
     1141 tcttttacgg ggacattcct cttactccaa agacttcccc tatagaagag gtgtgtcccc
     1201 atacctttta cttaggaact gtatcccagt cctttaaaaa ttggtctatc cctttatcag
     1261 tcaaaggatg ctttgcaagc tgctctatta ccttgtatgg tatggtggca atatggcacc
     1321 ctgattttgc agcctctatt acatgcatgg ggtgtcttat gcttgcagct atgatttcag
     1381 tatcaatttc atagttccag aaaatatcaa caatttcctt tataatagtc attccgtcat
     1441 taccgatatc atcaagcctt cccacaaaag gactcacata ggaagcacct gcttttgccg
     1501 caagaagagc ctggcctgcg gaaaatatca atgttacatt tgttttgata ttttccttgc
     1561 ttaaaacctt tactgccttt aacccttcaa ctgtcatggg aattttaata actatatttt
     1621 tgtgtatttt agccagctca acagcttccc taatcatccc ttctgagtcc aggcttataa
     1681 cctccgcact tatgggtccg tcaactatag aagtaatctc ccttaccaca tccttaaata
     1741 ttcttccttc ctttgctatc aatgacgggt ttgtggttac tccgcagata acccccatgt
     1801 catttgcctt tcttatttct tcaacattag ccgtatcaat aaataatttc atttttccct
     1861 cctcttgcgg ggacactggt gtcccttcta ttttattata atttgtttta ttctaaacct
     1921 gatgatgtgc attactttac tcgatttctc ttacaatgtc tttgtttccg aaaatcactt
     1981 taagcaagaa ttcaggcagc actatttgcc ttgttattct ctttggctcc ttaataagcc
     2041 tgtagaacca ttccagccct aaatttataa atatgtccgg tgcccttttc acaatgcctg
     2101 ccaggccgtc cagtgttccg ccgtttccaa taattacatt ggcatttaat aagtccttgt
     2161 tatattctat ccactgctcc tgttttttag caccaaatcc aacaaagaga atgtgaggtt
     2221 tatgtttgtt aatgtcttca attactgctc tttcttcctg gtggccatga tttccaagat
     2281 gagtgccctt gaaatatcca tgttgggctc caacaatttt aattccggga tacctttttt
     2341 gaacattttt catggcagct tcggctattc cgggttttcc ccccacggca taaagcttga
     2401 ggcctttttc atcggccatt ttcagcagat ttattgacaa gtcatagcct gccacctttt
     2461 cctttaaggg aagtttttta attttccccg cataaatcaa acccactccg tcaggaacat
     2521 tcacatccga cctgttcatt atatccagaa attcttcatc cttttgacac atcatgatta
     2581 tttccgtatt gggagtataa attacatact tttcatcatt ttcaatcttt tcttccgcag
     2641 cttttaacac ttcattcatt gatatattgt taattctaac acccattatg cttaatttat
     2701 ccattgctta tttcctttag tatcttatag ttaatattgg ttaattccct tattttatca
     2761 acactctttg aaagcattgc cttttcttcc tgctcattgt ttattttata tataatttcg
     2821 tcataaatag tattttcgtc aatatcgtca atcctgcagg ctattttcat attgacactg
     2881 tttacaaagt ggtccacctt gtggtcatat gaaacagcaa caataggaac gttggcggcc
     2941 gcagcaaaaa tcagcgaatg cagtctcact cccaccataa catccatggt tttaattatg
     3001 tcaaaagcat cccccgttga cagtctatcc ctgtaataaa aagccttgct gcctaatatt
     3061 tcttcgacct ggtcaatcag ctgcaaatcc tccttatgat aaaaaggtat gaagtgacat
     3121 tcaattccgt catcaataag cttttctgca accttgcata ttttagtgct gacatccgaa
     3181 cccttccaat tcctcagaga aaagcaaacc ttccttgtac tttgtttttc atattcaatg
     3241 gaattgtctt ttcttaaatt aattacagga tctgtagaaa gaaaaatctt cttctcatcc
     3301 actcctatgc tttccaaaaa ttcttttgaa tgcttatctc ttacagttat gtaatcaacc
     3361 agcgacaatg tgctcctgac tgcttttatg ctattttcgc atgtaatggg gccaatcccc
     3421 tggctcagca taacgacctt gtttttcata agctttgcta gccttatcag gaataaataa
     3481 taataaatgc ttcttttgct ggttacatcc tgtaaaaggc tccctccgcc aaaaataaga
     3541 acatctgcag attttagagc atttaatata gacagcacat tgtacctgtc aattgtgttt
     3601 acattgtatc ttttacttgt tattgccttg tttccggaaa gaactgttat attttctctg
     3661 tcggttattt caaatatctg tttcagtgtc atttccaata ttgcttcatc acctaaattg
     3721 ccaaagccgt aatagcctgc caacaacatt tttttcactt cagcctctcc tgtattttca
     3781 caaatgctct attacatata ctgcatacaa gaaccagtac acagcctata acaattccaa
     3841 agacaagggc gattaccgtt cttgcaaaag aaaggtaaat gggcgtcctt atatgagaaa
     3901 aagtatttat tacagaggat gtgccgattg cccccgccag cataaaaatt cccgtataaa
     3961 attcggactt tttgccagca gcaaaaacag ccccgaatat tgcaggaaat gctattaaaa
     4021 actccttggt tctaggtctt gccaaaagca tatattccat aaaatttctt gttatcattt
     4081 caatgtttga aggctgaatt tctgtttcat ggccggttct tgaaatataa atatatccgg
     4141 caagagcaag tatacctgca attatgccgt aataaatttt aattcccttg tttaaaaacc
     4201 ttacagtggt atttactacc cccttaaccg aatcctcgtc cttgttcata aagtagatta
     4261 taaacattat tatgaaaata ccgaagggca gtatctgggc aaattttact cccctgaaaa
     4321 tatccattt
//
LOCUS       DAOPUT010000116         4070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_22206_length_4070_cov_20.260523, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000116 DAOPUT010000000
VERSION     DAOPUT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22206_length_4070_cov_20.260523"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1316
                     /locus_tag="PKK75_07780"
     CDS             15..1316
                     /locus_tag="PKK75_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ83538.1"
                     /translation="MDYNQNLNENPENENQQNNILNDNSENENQQENQQDKTNHNMSN
                     EYGDVNFITGETYKKPGRKKSFMGYVALTLAAAIIGGLSGSYVTAVKLNEAAKEKENE
                     STISAQEVNINLNDDLYFAAAVAEKSQKTVVGITTEVEEQVNTFWGPQTRNAQSMGSG
                     FIVDSKGYIVTNAHVIGDGNYKNITVSLIDGSLETGQVMWYDTTLDLAIVKINKTGLP
                     SAELGNSDELLVGELAVAIGNPMTLDLERTVTQGIISGLDRSIQVENGTIIEPLIQTD
                     ASINSGNSGGPLFNAKGQVIGINAAKMMTAEGLGFAIPINIIKPVLDQIITDGTFEPV
                     YVGISGMDVETYEMLYGVDLTADYGVVIIETMKNSPAKEAGLQPNDVIIQIDGKKTEN
                     MTDIQRKLYDYQEGDKAEFKILRNGEEETITVTLKNRPEGV"
     gene            complement(1431..2096)
                     /locus_tag="PKK75_07785"
     CDS             complement(1431..2096)
                     /locus_tag="PKK75_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HNZ83539.1"
                     /translation="MDPKKVIAKRVAELLHDGDVVNLGIGLPTMVANFIPEGVDVTFQ
                     SENGFLGLGPAPKEGEEDWELVNAGAMPSTIVPGGMFFDSATSFSIIRGGHVDATVLG
                     AMEVDEKGNLANYKIPGKMVPGMGGAMDLVVGARTVIIAMVHTQKGKPKILKDCTLPL
                     TAKGQVDIIVTEMALMRVTKDGLVLEELGPEATVEDVVAATEANLIVSPDLKRFGTEF
                     LDQ"
     gene            complement(2096..2749)
                     /locus_tag="PKK75_07790"
     CDS             complement(2096..2749)
                     /locus_tag="PKK75_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNZ83540.1"
                     /translation="MNKVISVKEAADMVKEGMTVMVGGFLANGTPENIIDALVEKNVK
                     NLTLICNDTGFPDKGVGKMVVNKQFKKMIASHVGTNPETANQMNSGETEVVLTPQGTL
                     AEKIRCGGNGLGGFYTPTGIGTEVAEGKEIKVINGKEYLFETPLRADVALLRASVADK
                     KGNMIFNKTMKNFNPIMATAADLVIVEADKIVEVGEINPEHVMCSGIFVDYIVGGGK"
     gene            complement(2805..3983)
                     /locus_tag="PKK75_07795"
     CDS             complement(2805..3983)
                     /locus_tag="PKK75_07795"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNZ83541.1"
                     /translation="MKKAVIVSAARTPIGSFGGAFASLTAVDLGVIAAKEAIKRAGIE
                     PNMVEEVYLGNVLGAGLGQNVARQVCLGAGIPVETPALTINMVCGSGLRAVSMAAQFV
                     ESGQCDIILCGGTESMTDAPYLLPKARWGHRMGDGKVVDYMVHDGLWDIYNDYHMGIT
                     AENVAEQYGLTREEQDSFAAQSQIRAEEAQKAGRFADEIVPVIIPQKKGDPIVIDTDE
                     YPRHGSTVEKMAKLKPAFKKDGTVTAGNASGINDGAAALIIMSKEKADELGLKYLCEI
                     AGYGSAGVDPKVMGLGPIPATKKALANAGWTIDDIELAEANEAFAAQSLAVVRDLGLK
                     PEITNVNGGAIALGHPIGASGARILVTLIYEMQKRDVKKGLATLCIGGGMGTSLLVER
                     "
BASE COUNT         1195 a          906 c          719 g         1250 t
ORIGIN      
        1 aggagtgatg aacaatggat tacaatcaaa atttaaatga aaatccggaa aatgaaaatc
       61 agcaaaataa tatattgaat gataattcag aaaatgaaaa ccaacaagaa aaccaacaag
      121 acaaaacaaa tcataatatg tcaaatgaat acggcgatgt taatttcatt acgggagaga
      181 cttataaaaa gcccggacga aaaaaatcat ttatgggtta tgtggccctc acattggcgg
      241 ctgcaataat cggaggtcta tcaggttctt atgtgactgc tgtcaagctg aatgaagcag
      301 caaaagaaaa agaaaatgag tcaacaatca gcgcccaaga ggttaatata aatttgaatg
      361 atgatttata ttttgcagca gcagtagccg aaaaatcgca aaaaaccgtt gttggaataa
      421 caactgaagt tgaagagcag gttaatacat tctggggacc ccaaaccaga aatgcacaaa
      481 gcatgggctc cggctttatt gtagattcaa aaggatacat tgttaccaac gcacatgtaa
      541 tcggtgacgg aaattataaa aatataacag tttcattaat tgacggttca ttagaaacag
      601 gtcaggttat gtggtatgat acaacattgg acctggcaat agttaaaata aacaagacag
      661 ggcttccgtc tgcagaatta ggaaattctg atgaattgct ggttggagag ctggcggttg
      721 ccataggcaa tccaatgacc ctggacctgg aaagaacagt tacacaaggc ataataagcg
      781 gtctcgacag atccatccag gttgaaaacg gaacaataat tgaacccctt atccagactg
      841 atgcatccat aaattcaggc aacagcggcg gtcccctgtt taacgcaaag ggacaggtaa
      901 tagggataaa tgcggctaaa atgatgacag ctgaaggatt gggttttgca ataccaatca
      961 atatcataaa acctgtttta gatcaaataa taactgacgg tacctttgaa ccggtatatg
     1021 tgggcatctc aggcatggat gttgaaacct atgaaatgct ttacggagtt gacctcacag
     1081 cagactatgg agtagttata atagaaacta tgaagaattc ccctgcaaag gaagccggtc
     1141 ttcagccaaa tgatgttatt attcaaattg acgggaaaaa gacagaaaat atgacagata
     1201 tacaaagaaa actatatgat tatcaagaag gcgacaaggc ggaatttaaa attttaagaa
     1261 acggtgaaga ggagaccatc acagtaactt taaaaaacag accggaaggg gtataatata
     1321 aagtgtctta agaattcatg ctgattacac tttattatgg tcagatggta ttaacaggat
     1381 aataaaaaca ggagatttct cctgtttttt tatcctcata tcgaaaagat ttattgatct
     1441 aaaaattcag ttccaaagcg tttcaagtca ggtgaaacga ttaaatttgc ttctgttgca
     1501 gcaacaacat cctcaacagt tgcctcaggg cctaattctt caagaaccaa tccatccttt
     1561 gtcactctca taagggccat ttcagttact attatatcaa cctgtccctt agctgttaaa
     1621 ggaagagtgc aatctttcaa tattttcggc tttccctttt gtgtatgaac cattgcaatt
     1681 ataactgtcc tggcacctac aactaggtcc attgcgcctc ccatgccggg aaccatcttg
     1741 ccgggtattt tatagtttgc cagatttcct ttttcatcaa cttccatggc acccaatact
     1801 gttgcatcca catgtccgcc tctgataata ctgaaggagg tggcgctatc aaagaacatt
     1861 ccaccgggca ctattgtaga aggcatggct cctgcattta caagttccca atcttcctcg
     1921 ccttccttag gagcaggacc taatcccaaa aatccgtttt ctgactggaa agtgacatcc
     1981 acaccttcgg gtatgaaatt tgcaaccatt gtaggaagcc ctatgcctaa gttgacaacg
     2041 tctccgtcat gcaagagctc agcaactctt tttgctataa ctttctttgg atccattatt
     2101 tgccacctcc aactatataa tcaacaaata tacctgaaca catgacatgt tccggattaa
     2161 tttcccctac ttcaacaatt ttatcagctt caacaatcac cagatctgct gctgttgcca
     2221 taatcggatt gaagttcttc atggttttgt tgaatatcat attgcctttt ttgtcagcta
     2281 cagaagctct caataatgcc acgtctgccc tcaagggagt ttcaaacaag tactctttac
     2341 cgtttattac tttgatttcc tttccttctg caacttcagt tccgattcca gttggcgtgt
     2401 aaaagcctcc aaggccgttt cctccgcatc tgattttttc cgccagggtt ccctgaggtg
     2461 tgagcactac ctctgtttcc cctgaattca tttgatttgc agtttcagga ttagttccta
     2521 catgtgatgc aatcattttt ttaaactgtt tattaacaac catttttccc acgcctttgt
     2581 cgggaaaacc ggtatcattg caaataagag taaggttttt aacattcttt tctaccaatg
     2641 catctattat gttttccggc gttccatttg ccaaaaaccc gcctaccatt acagtcatgc
     2701 cttccttaac catatctgcc gcttctttga cagaaattac tttgttaatc atactttcat
     2761 cctttcttta taaattatat atccgtagaa atacctttat atttttatct ttcaaccaat
     2821 aatgaagttc ccatgccgcc gcctatgcac aaggttgcaa ggcctttttt aacatctctt
     2881 ttttgcatct catatataag agtaacaagt attcttgccc cggatgctcc tatggggtgt
     2941 cccaatgcta ttgctccgcc gttaacattt gtaatttcag gttttaaccc taagtcgcga
     3001 acaactgcca gtgattgtgc agcaaaagct tcattagctt cagcaagctc tatgtcatca
     3061 attgtccagc ctgcatttgc caatgctttt ttagttgcgg gaatcggtcc taatcccata
     3121 accttcggat caactcctgc agagccatag cctgcaattt cgcacaaata tttcaatcca
     3181 agctcgtctg ccttttcttt gctcataata atcaaggctg ctgcaccgtc atttataccg
     3241 gatgcatttc ctgctgtaac agttccgtcc tttttgaatg caggttttaa ttttgccatt
     3301 ttctcaactg ttgagccgtg tctcggatat tcatcagtat caattactat agggtctccc
     3361 ttcttttgag gtattataac aggcactatt tcatctgcaa atcttcctgc tttttgagcc
     3421 tcttctgctc taatctggct ttgtgctgca aagctgtcct gctcctctct agtaagtcca
     3481 tactgctcag caacattttc agctgttatt cccatgtggt aatcattgta aatatcccac
     3541 aatccgtcat gtaccatgta atctactacc ttgccgtctc ccatcctatg tccccatctg
     3601 gcctttggca atagatacgg agcatcagtc atgctttctg taccgccgca taaaattata
     3661 tcacattgac cgctttcaac aaactgtgct gccatgctta cagctcttaa gccggagccg
     3721 cataccatat taatcgttaa tgctggagtt tcaacaggaa tacctgctcc taagcaaact
     3781 tgtcttgcaa cgttttgtcc caagcctgcc cctaacacat tacctaaata aacttcttcc
     3841 accatgtttg gctcaattcc tgcccttttg atggcttcct ttgctgctat aactcccaaa
     3901 tctactgctg tcaatgaagc aaaagcacct ccgaaggatc ctataggtgt tcttgctgct
     3961 gaaacaatta ctgctttttt cattttttcc tcctaaattt ttatttctat tgttattact
     4021 tgatattttg tatttcatct atgtatataa tatagcattt ttcatgccaa
//
LOCUS       DAOPUT010000117         4043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_22393_length_4043_cov_19.892678, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000117 DAOPUT010000000
VERSION     DAOPUT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22393_length_4043_cov_19.892678"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKK75_07800"
     CDS             <1..240
                     /locus_tag="PKK75_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore gernimation protein"
                     /protein_id="HNZ83542.1"
                     /translation="AEVGAYIQYDEVSQAPYFIYYDQQGRQHRVWFEDARSIMAKLNL
                     FSEYGFEGVGYWNIMRYFPQNWLLVSNLYDIARLI"
     gene            318..1508
                     /locus_tag="PKK75_07805"
     CDS             318..1508
                     /locus_tag="PKK75_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNZ83543.1"
                     /translation="MNNKNYRVLKFFEPVNKKDNSKGYVRLDMRGNKGNMIVAAENLG
                     DPKSTSEVYLYKDKHNKIKLGDINNKKGIIKKILTFGNSNAIEDYNICAVVKDGKIVL
                     YSNLFSTSSPDMVRKLEEDADEPEKNIFADEKQFRESKEPKPKADEQEYMKAAKEVLE
                     PKEIIEAKGLREIRAIKEKDIMRAAEKESAGQIKEDIKKVVEEPVKTIKIPVKKEKEE
                     VKQAGKEETGEETKEEAKEAEELAVQQRYKNRFDEKLYKTLNAYKEVEPLSVRLKDFK
                     WWYIPYDETGMKTGILPYYNQIISSYYPYPMSARVTTCASLMKKYGHYIFGICKDKDQ
                     ITRFIYGVPGGFTKEEQPYKGVTGFKNWSYAREDNKEGYGYWLAFVNPKTGETTDPPQ
                     IQLI"
     gene            1613..2260
                     /locus_tag="PKK75_07810"
     CDS             1613..2260
                     /locus_tag="PKK75_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNZ83544.1"
                     /translation="MKYKYVIFDFDGTLADTEEANFIIYQKLAEKYNLRNITMDELGH
                     LKKMNARDVMAYVELKKRYLPFILKKGKKLLTNDIQNISLCKPDIFQIIGRLRDMGVK
                     TAIITTNSKANVKLFLEAHNVDAFDLILSAAMFGKESKMKKIVKKEKIKLHEFLYVGD
                     EIRDITAARHAGMDIASVGWGYNTVESLKKNEPDYLIFEPSELIEICQGTYVKGI"
     gene            2267..>2508
                     /locus_tag="PKK75_07815"
     CDS             2267..>2508
                     /locus_tag="PKK75_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNZ83545.1"
                     /translation="MNKMIYTIKPEYHDIENLNKILGDHQEIQFVSFMGVDIGGNGTD
                     EKIPVKLFIKDMEEMLRYGIQTDGSSVELQGIADLS"
     assembly_gap    2509..2608
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2609..3911
                     /locus_tag="PKK75_07820"
     CDS             <2609..3911
                     /locus_tag="PKK75_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNZ83546.1"
                     /translation="EQYWKRTTGTVRTVLEKIMGLMECYDLKPEMAHKEVGGITNRID
                     AKGRITHVMEQLEIDWRFSNPLQAADNEIFIKELIEDQFRYNGLEVLFKAKPIEGVTG
                     NGEHTHLGAAVRLKDNRKINLFSPENMKKDYLSEIGYGALMGILKNYEVINPIVSAAN
                     DAFNRLKPGFEAPVCTVTSLGGTKDAPSRNRSVLIGLIRNIDNKSVTRFELRSPNPYS
                     NTYLVIAACIQAMLDGIVSVIDAGMDCRMLEKEISKKYGEDGIYLEKFREYRSEFDVF
                     VYYSQEERDKLYGKAPTTLWENINAFSSNQIKQQVLKKGDVFTEEIINSFRISTAEKW
                     KNELAGRIIHDNIVLLKTFVKLHSESDHATDLDVVNWEKIVYLKTKLMKDSMTKKCIF
                     TKIKTAISQGDYDTASDLQIQMNEKMSEIRTLYIGYKNNIF"
BASE COUNT         1511 a          563 c          841 g         1028 t
ORIGIN      
        1 gctgaggttg gggcatatat tcaatatgat gaggtatccc aagctcccta cttcatatat
       61 tatgaccagc agggaaggca gcacagggtt tggtttgaag atgcaagaag catcatggca
      121 aaattgaatt tattcagtga atacggcttc gaaggtgtcg gctactggaa cattatgaga
      181 tattttcccc agaactggct tttggtaagc aacttatacg atattgcaag gcttatataa
      241 tcaaagcttg ccataaaaat gtaattatac gaaaaaatca gcatataaat aaataacagg
      301 tatagtagga ggtttttatg aacaataaaa attatagggt gctgaaattt tttgaaccgg
      361 ttaataaaaa agataattct aaaggatatg tacgtctgga catgagagga aacaaaggaa
      421 acatgattgt agcagctgag aatttaggtg acccgaagtc aacaagcgaa gtttatttat
      481 ataaggataa acacaataaa atcaagctgg gagatataaa caataaaaaa ggaataataa
      541 agaaaatact tacctttgga aacagcaatg ctattgagga ttacaacata tgtgcagtgg
      601 tgaaggatgg aaagattgtt ttgtattcaa acctattcag tacatcaagc ccggacatgg
      661 taagaaagct ggaagaagat gctgatgagc ccgaaaaaaa tatttttgca gatgaaaaac
      721 aattccggga gtcaaaagag ccaaaaccta aggcagatga gcaggaatat atgaaggccg
      781 caaaagaagt actagagccc aaagaaataa ttgaagccaa agggttacgg gagataaggg
      841 caataaaaga aaaagacata atgagggcag cagaaaagga atcagccggc caaataaaag
      901 aagatattaa aaaagtggtg gaagagccgg tgaaaacgat aaaaatacct gtaaaaaagg
      961 aaaaagaaga agttaaacaa gcaggaaaag aggaaacagg agaagagaca aaagaagaag
     1021 caaaggaagc cgaggagctc gctgttcagc aaaggtacaa aaatagattc gatgaaaaat
     1081 tatataaaac attaaatgct tataaagaag ttgaaccttt atcagtcaga ttaaaggatt
     1141 ttaaatggtg gtatataccc tatgatgaaa ccggcatgaa aacagggata ctgccttatt
     1201 acaatcagat aataagctca tattatccct atcccatgtc tgcccgtgtg accacctgtg
     1261 caagtctcat gaaaaaatac ggccactata tttttggtat ctgtaaggat aaagatcaaa
     1321 ttacaaggtt tatttatggt gtgccaggcg gatttaccaa ggaagaacag ccatataagg
     1381 gtgtgaccgg ttttaaaaat tggtcatacg ccagagaaga caataaagaa ggctacggat
     1441 actggcttgc ctttgtgaat cctaaaaccg gtgaaaccac agacccaccc cagattcaac
     1501 tgatataatt cttaattctg taaaaaaact cctttaagga gtttttttaa attgactgat
     1561 taaaaaaaat ataatatagt taataaaaaa attaatatta aggatgattt gggtgaaata
     1621 taaatatgta atttttgatt ttgatggaac tttggctgat actgaagaag caaatttcat
     1681 tatttatcaa aagttagctg aaaaatacaa cttaagaaat attacaatgg atgagcttgg
     1741 tcatctaaag aagatgaatg ccagggatgt tatggcatat gttgaactta aaaaaagata
     1801 ccttccgttc attttaaaga aagggaaaaa acttttaact aatgacatac aaaatataag
     1861 tctctgtaaa cccgatattt tccaaataat aggcaggctt cgggatatgg gtgtaaaaac
     1921 agcaataata actactaatt ccaaagctaa tgtgaaactt ttcctggaag cacataatgt
     1981 tgatgcattt gatttgattt taagtgccgc aatgtttggc aaggaatcaa agatgaagaa
     2041 aattgtaaaa aaggaaaaga taaaactaca tgaatttctt tatgtgggag atgaaataag
     2101 ggacataaca gctgcaaggc atgcaggcat ggatatagca tcagtaggct ggggttataa
     2161 tacagttgaa agtcttaaaa aaaatgaacc ggattattta atattcgaac cctctgagct
     2221 tatagagatt tgtcagggga catatgtaaa ggggatataa aagtttatga acaaaatgat
     2281 ttatacaata aagcctgaat atcacgatat tgaaaacttg aataaaattt taggagacca
     2341 tcaggaaata caatttgtat cctttatggg agtagatata ggcggaaacg gtacagacga
     2401 aaaaataccc gttaaattgt ttataaagga tatggaggaa atgcttagat acggcataca
     2461 gacagacgga tccagcgttg aacttcaggg tatagctgat ttaagcgann nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg agcagtactg gaaaagaaca acgggaactg
     2641 taaggacagt tttggaaaaa ataatgggat taatggaatg ctatgattta aaaccggaaa
     2701 tggcacataa ggaagttggt ggcattacca atagaataga tgcaaaggga agaataaccc
     2761 acgtaatgga gcagctagaa attgactgga gattttcaaa tcctctccag gcagctgaca
     2821 atgaaatttt cataaaggag ctaattgaag accagttcag atataacggt ttggaagtat
     2881 tattcaaagc caagcccata gagggtgtta cgggaaacgg agagcatact cacttggggg
     2941 cggcagtaag gcttaaagat aatagaaaaa taaatttatt ttctcctgaa aacatgaaaa
     3001 aggattattt aagcgaaata ggctatggtg cccttatggg tatactgaaa aattatgaag
     3061 taataaatcc catagtgtct gctgcaaatg atgcctttaa caggcttaaa cccggatttg
     3121 aagcccctgt gtgcactgta acctccttgg gaggcaccaa ggatgcacct tcgagaaacc
     3181 ggtctgtttt aattggccta atcagaaata ttgacaataa aagtgtaacc aggtttgaat
     3241 taaggtctcc taatccttat tccaacactt accttgttat agctgcatgt atccaggcaa
     3301 tgttagacgg aatagtgtca gtaatcgatg caggaatgga ttgccggatg ctggagaaag
     3361 aaatatcaaa aaaatacggc gaggatggga tctacctgga aaagttccgg gaatacagaa
     3421 gcgaatttga tgtatttgtg tattattcac aggaagaaag agataaactt tacggaaagg
     3481 cacccacaac cctttgggaa aatataaatg cattttcctc taaccagatt aaacagcagg
     3541 ttttgaaaaa gggcgatgtt tttacggaag aaatcattaa ttccttcaga atatcaacag
     3601 cggaaaagtg gaaaaatgag cttgcaggaa ggataataca tgataatata gtactgctaa
     3661 aaacatttgt gaaactccat agcgagtcag accacgctac agatttggat gtcgtaaatt
     3721 gggagaagat tgtttattta aaaacaaagc ttatgaagga cagcatgact aaaaagtgta
     3781 tatttaccaa gattaaaaca gcaatatccc agggagatta tgatacagca tctgatttgc
     3841 aaatacagat gaatgaaaaa atgtctgaaa taagaacact ttacattggg tataaaaaca
     3901 acatatttta aaggttgggg gaaatgtctt cttctaaagg ggcagttttt gcagatcgaa
     3961 ggatgtccct ttttttgttt ttttataatc tatttgttta tttattaatt tccacaaaat
     4021 gttgtttaca aaactgttaa cat
//
LOCUS       DAOPUT010000118         3977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_22907_length_3977_cov_22.449261, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000118 DAOPUT010000000
VERSION     DAOPUT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22907_length_3977_cov_22.449261"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKK75_07825"
     CDS             complement(<1..365)
                     /locus_tag="PKK75_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ83547.1"
                     /translation="MRNIIIGTAGHIDHGKTTLIKALTGRETDRLEEEKRRGITIDLG
                     FTYFDLPDGSKAGIIDVPGHEKFIKNMLAGVVGMDMVLLVIAADEGIMPQTTEHLNIL
                     NILGLENGIVVLTKCDLAD"
     gene            complement(381..1781)
                     /gene="selA"
                     /locus_tag="PKK75_07830"
     CDS             complement(381..1781)
                     /gene="selA"
                     /locus_tag="PKK75_07830"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNZ83548.1"
                     /translation="MDLNKYYRKIPKVDSILENEKIKKLLEKTSRELVLDCVREATED
                     LRKLINDKPDNEDEINHEINNLIGKIALMLDRLTNYNMKKVINGTGTILHTNLGRAPI
                     SKEAAERARNIVTGYSNLEFDLEQGKRGSRYSHFEKIIAKITGAEAALAVNNNAAAVL
                     LILSALAKDKEVIVSRGELVEIGGSFRVPDVMKQSGGILVEVGTTNKTHLHDYEERIT
                     PDTAALLKVHTSNYKIVGFTESVSIEELSALGKKFNIPVIEDIGSGVLTDLSSYGLTY
                     EPTVQNSIKAGIDIICFSGDKLLGGPQAGIIAGKKHYIDKIKKHPLNRALRIDKFTAA
                     VLESVFHEYLEEEKAMENIPVLKMINMNIEDIKQSAEKLSKKLKDFSEFLDIGIDECL
                     SQMGGGALPLERMKSYAVSLKPKTMTCTSFERELRNIDVPVIGRVLNDTFYIDLRTVL
                     EGEDQVIYDGIKNLFK"
     gene            complement(1860..2444)
                     /locus_tag="PKK75_07835"
     CDS             complement(1860..2444)
                     /locus_tag="PKK75_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HNZ83549.1"
                     /translation="MNNSVYKKGLAVVLIIAIIIMAALGIKLSGRDEEIIFTNYSSID
                     ANESDEINEGIKEEAKVYIYVDMDGAVVNPGVYGLIEGSRVIDAINMAGGLTEKAFTQ
                     NLNKARKLVDGEKIFINEEGEAADVFIDDKLININTASEDSLMSLPGIGEVYAKRIIE
                     YRNAKRFNSIEEIKNIQGIGDKTFDKLKDLITIN"
     gene            2672..3958
                     /locus_tag="PKK75_07840"
     CDS             2672..3958
                     /locus_tag="PKK75_07840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNZ83550.1"
                     /translation="MIKRILFYILIFSCFTVNFNTVYASPVILSESAVLIDGVTGTVL
                     AHKNADKKMYPASLTKIMTAILALEKGKLTDVITVDDDTPFEIDGSHIALEPGEILTL
                     KDLLYALMLPSANDAASVIAKHYGGSLENFVKLMNEKAKEIGAYNTNFTNPHGLHDTN
                     HYSTAADLALITKYAMGNETFRKIVSTTKYEIQTTNKKNEPRYFTNLNKLIYNTSYNQ
                     IVVDGVYVSPYYEYATGAKTGYTPQAGNCLVATAKKDGTELIAVTMKGISLEMYQDAH
                     NLFNLGFEEYVNAKLAGKNTFIKNIKISNGDSKEISLITESDLTALVEKSSLDNIKSN
                     IYIDEISLPIEKNQIVGKIEYTLDSEVIGTVNLISPISVKSTSKEGQGNIFLSIVKFI
                     GYLLLFAAVSILLLKVYNDIRIRVNRKKRRSRYNNQ"
BASE COUNT         1219 a          821 c          596 g         1341 t
ORIGIN      
        1 tggtcagcca aatcgcattt ggttagaaca acaattccgt tttcaagacc cagaatattt
       61 aatatattta aatgctcggt agtctggggc ataataccct catcggctgc aatgaccaaa
      121 agaaccatat ccattcccac tacacctgca agcatgtttt taataaattt ctcatgaccg
      181 ggcacatcta ttatacctgc cttgctgcca tcaggaagat caaaataagt gaatcccaaa
      241 tcaattgtta ttcctctcct tttctcctct tccagcctgt ctgtttctct tcctgtaaga
      301 gcttttatca atgtggtttt cccatggtct atatgtcctg ctgttccgat aataatattt
      361 ctcatacagc ctccaaaata ttatttgaat aaatttttta tcccgtcata aattacctgg
      421 tcttcacctt ctaaaacagt tctcaaatct atgtaaaaag tatcatttaa aactcttcct
      481 ataacaggaa cgtcaatatt tctcaattcc ctttcaaagc ttgtgcaggt catcgtctta
      541 ggttttaagg aaactgcata gcttttcatc ctttccaggg gaagggctcc tccacccatt
      601 tgagacaggc attcatcaat gccgatatct aaaaattcag aaaaatcctt gagctttttg
      661 gaaagttttt ctgcactttg ttttatatcc tctatattca tgtttatcat tttaagcaca
      721 ggaatgtttt ccattgcctt ttcttcctca agatactcat gaaatacact ttccaatact
      781 gctgctgtaa acttgtcgat tcttaatgcc ctgtttaagg gatgtttttt tattttatct
      841 atataatgtt ttttcccggc tattattccc gcctgtggtc ctcccaaaag tttgtctccg
      901 ctgaagcaaa tgatgtctat tcctgctttt atggaatttt gaactgtagg ctcatatgtt
      961 aaaccgtaag agcttaaatc tgtcaatacc ccgcttccta tatcttcaat aaccggaata
     1021 ttgaattttt tgcctaaagc ggataattcc tcaattgaaa cactttccgt gaaaccaaca
     1081 attttataat tgctggtatg aacctttaat aaagctgctg tatcaggagt tattctttct
     1141 tcataatcat gaaggtgagt tttatttgtg gtacccactt caactaaaat tccgccgctt
     1201 tgcttcataa cgtcaggaac tctgaaggac ccccctattt ctaccagctc acctcttgaa
     1261 actatcactt ccttgtcctt ggcaagagct gacaaaatca aaagcactgc agctgcattg
     1321 ttatttactg ccaatgctgc ctctgcccct gtaattttag cgattatctt ttcaaaatgt
     1381 gaataccggg accctctttt gccttgttcc aaatcaaact ccaggtttga atatcctgtt
     1441 acaatattcc tggctctctc ggctgcttcc tttgatatgg gagccctccc cagattagta
     1501 tgtagaattg ttcccgtgcc gtttataacc tttttcatat tataatttgt cagtctgtct
     1561 aacattaatg caattttccc tattaggtta tttatttcat gatttatttc gtcctcgttg
     1621 tcaggcttgt cattgataag tttccttaag tcttctgttg cttccctcac acagtccagc
     1681 accaattctc tgcttgtttt ctccaatagt ttttttattt tttcattttc cagaattgaa
     1741 tcaaccttgg gaattttcct gtaatactta tttaaatcca tttcataccc tcccgcaggg
     1801 acatattcct attgaccacc atcaaaaaat gcttgtcaaa taaaggtgcc tgcccttact
     1861 tagtttattg ttattaaatc tttaagctta tcaaaagttt tatcccctat accttgtatg
     1921 ttttttatct cttctatgga attaaacctt ttggcatttc tgtattcaat aattcttttt
     1981 gcatatactt caccgattcc gggcaaagac ataagagaat cctcagatgc agtatttatg
     2041 ttaattaatt tatcatcaat gaaaacatct gctgcttccc cttcctcatt aataaaaatt
     2101 ttttctccgt caaccaattt cctggctttg tttaaatttt gagtaaatgc tttctctgtg
     2161 aggcctcctg ccatatttat tgcgtcaata accctgcttc cttcaatcag cccgtaaacc
     2221 ccgggattaa caaccgctcc gtccatgtca acataaatat aaacttttgc ttcttcctta
     2281 atcccctcat taatttcatc actttcattt gcatcaatac ttgaataatt ggtaaaaata
     2341 atttcttcat ccctgccgga caatttaatc cccaaggcag ccataataat tattgcgatg
     2401 attaaaacaa cggcaagtcc cttcttatag acgctgttgt tcatatttcc ccctatatgc
     2461 ttttcaggaa tattcctctg acttaaaagt tttacccttt agaaaagagg tttgtcccca
     2521 tacccctatt ggtatgttcc ttcgccttct attatagtac acacattata cattatttgt
     2581 ctaaaaaaaa caaaatattt gattacaaat atatattttt ctgctatact attatttgac
     2641 tatttacatg gaacgaattt aggagttacg catgataaaa cgaatactgt tctacatatt
     2701 aatcttctct tgttttacag ttaatttcaa cacagtatac gcatcccctg ttattttaag
     2761 tgaatctgct gttttaattg atggcgtcac aggtaccgta ttggcacata aaaatgctga
     2821 taaaaaaatg tatcctgcca gcttaacaaa aataatgact gctattcttg ccttggaaaa
     2881 gggaaaatta actgatgtaa ttactgtgga tgatgatact ccctttgaga ttgatggaag
     2941 ccacatagca ttagaacccg gagaaatact gactttaaaa gatttactct atgcattgat
     3001 gctgccttca gcaaatgatg ctgcctctgt aattgccaag cactacggcg ggagtttgga
     3061 gaatttcgta aaattgatga atgaaaaggc aaaggaaata ggagcataca acactaattt
     3121 tacaaatccc cacggactgc atgatacaaa tcattactca actgcagccg acttggcact
     3181 aattacaaaa tatgcaatgg gaaatgaaac attcagaaaa attgtgtcaa ccacaaagta
     3241 tgaaatacaa acgaccaaca aaaagaatga accaagatat ttcacaaatt taaataaatt
     3301 aatatataac acaagctaca atcaaattgt tgttgacgga gtatatgtaa gcccatatta
     3361 tgagtatgca acaggagcaa agacaggata tactccacag gcaggaaact gtctggttgc
     3421 aactgccaaa aaagacggaa ctgaacttat agccgttacc atgaagggaa tttcccttga
     3481 aatgtatcag gatgctcata atcttttcaa tcttggtttt gaagaatatg taaatgcaaa
     3541 attggcgggt aaaaacacct ttataaaaaa tattaaaata agcaacggag attcaaagga
     3601 aatatctctt attacagaaa gcgatttaac cgctcttgtt gaaaaaagct cgttggataa
     3661 cattaaatca aatatatata ttgatgaaat aagccttccc attgaaaaaa atcaaattgt
     3721 agggaagatt gaatatacac ttgacagcga agtaatagga acagtaaatt taatatcacc
     3781 catatcagta aaaagcacgt caaaagaagg acaaggaaac atttttcttt caattgtaaa
     3841 atttataggt tacctccttt tatttgctgc agtttcgata ctgctgctaa aagtttacaa
     3901 tgatattaga attcgggtaa acaggaaaaa acggcgaagc aggtacaata atcagtaata
     3961 aaaaagttgc aggattt
//
LOCUS       DAOPUT010000119         3935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_23270_length_3935_cov_15.050515, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000119 DAOPUT010000000
VERSION     DAOPUT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23270_length_3935_cov_15.050515"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..329
                     /locus_tag="PKK75_07845"
                     /pseudo
     CDS             54..329
                     /locus_tag="PKK75_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
     gene            343..1410
                     /locus_tag="PKK75_07850"
     CDS             343..1410
                     /locus_tag="PKK75_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNZ83551.1"
                     /translation="MIVFNDLLEEFEEARRNAYISAITLKGQGKIVAGIFGKNIPREI
                     LWALEITPVNVYSIDGSNICESEGFIDSGRCSLVKASYGYTVTERCPLIYSSDIIISN
                     NACPHKTSMISKLSGFKESYIIEDYKEVEKQAAEYKKFAEYLENKFNKRLNKQALHSV
                     IQKTNKINTMTKKIMEMYMSGEYPISVYDLHSIIYGSQFFLDLDERYAKLSQMSDALE
                     NLRIEKTPMEKNNIKKVFITGAPLAGLTQEILKPLSIMKNIFVVLSQSLCEGENYQPI
                     NEGENPYLNLAEKYIRDDDEMNEFLRGNDTGAIIDVKLSGCDVLKITEMNTSLPSLSL
                     TVDYDYDIEKINKALHFFIHN"
     gene            1533..2810
                     /locus_tag="PKK75_07855"
     CDS             1533..2810
                     /locus_tag="PKK75_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HNZ83552.1"
                     /translation="MADYRKMWEDLGMDLELHDLLCDALPVAFGDVYMSMENRPESMD
                     FYDFVVSEIHGVRPAELVEFQKKGGKVFGTFCVYVPDEIVFAANGIATGLCGGSQFWV
                     PGGEKVLPTNTCALIKASVGARLERTCPFFRIADIYVGETTCDGKKKAWEILAEDVPV
                     HIMDMPQMKRPQDTAKWIEEIKIFKDKVEEVTGNEVTAEKLSEAIKLINNKRKALARL
                     YELRKEKNIPISGKDVLLISQIAFYDDPTRFTQMTNKLCDELDERVKNNISVFPEGTK
                     RIMITGTPMAIPNWKMHHIVETSGGAVVVEEACTGTRYFENLVDESKDTMDEQIAALA
                     DRYLKNNCACFTPNTGRIDDILRLAKEYKVDGIIDVNLKFCNLYDTEGYFVEKALKEA
                     GIPVLGIETDYTDQDAEQLRTRVGAFIEMLSNK"
     gene            2812..3066
                     /locus_tag="PKK75_07860"
     CDS             2812..3066
                     /locus_tag="PKK75_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNZ83553.1"
                     /translation="MEYYILFNDVDSGLKLYNLLKSAKIKVTISPTPREASKCCGISL
                     LIKNKDDLPSIRECIENNNIEIKDIFQMESKRDPKRDKFC"
     gene            3343..3843
                     /locus_tag="PKK75_07865"
     CDS             3343..3843
                     /locus_tag="PKK75_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNZ83554.1"
                     /translation="MKYFIGVPIPKSYKNKIEMLRAEFRFLTTEPHITLVPPPALPDE
                     DDFVEKVVNVCKTIEPFDIRLEGLDQFATRVLYVSVDSPGLIDLYKKIYENLGLQQER
                     RGFTPHLTVVKQRQGRPVDIDSIRKRAEIKLIPYPEYTLNSITVYHQPKEHSIYVPYM
                     EIPLGK"
BASE COUNT         1399 a          599 c          803 g         1134 t
ORIGIN      
        1 tattaaaatt aaatcaaaat taattttagg ggtgacatat gtattatgtt ggaatctgta
       61 ccgtatttgc agagtcagag gtaatcagtc taatcggcag cggctgctct aaaaatgata
      121 ttgccagcgg tgtggttaat tccattgcaa acaaagtcta tacactttgt gaaaaacata
      181 atgccaaaac caaagagtat tttctcacag gaggcttttg cgacagcagg tacatgtgtg
      241 acaagctcag ccagaaattg aaaaggcctg tgacaaccac tgaattggcc cggtatgcag
      301 gtgcaatagg ggctgcactt ttagcctgag aagggatttg taatgattgt ttttaatgat
      361 ttgctggaag aatttgaaga agctaggaga aatgcctata tatctgccat aaccttaaaa
      421 ggtcagggca aaattgttgc ggggatattc gggaaaaaca tacctagaga aattttatgg
      481 gcattggaaa taacacctgt caatgtatac agcattgacg ggtctaatat ttgtgagtcc
      541 gagggattta tagattcagg caggtgttct cttgtcaagg caagctatgg atatacagtc
      601 acagagaggt gccccctcat atattcgtcg gacataataa taagcaataa tgcatgtcct
      661 cataaaactt caatgatttc caaattaagc ggatttaaag aaagctatat cattgaagac
      721 tataaggaag ttgagaaaca agcagcagaa tataaaaaat ttgctgagta tttagaaaac
      781 aagtttaaca agaggttaaa caagcaagcc cttcattctg ttatacagaa aacaaacaaa
      841 ataaatacaa tgacaaaaaa gattatggag atgtatatgt caggagaata tcctataagt
      901 gtctatgatt tacattcaat aatctatgga agccaatttt ttttagattt ggatgaaagg
      961 tatgctaagc tgtcacaaat gtctgacgca ttggaaaact taagaattga aaaaacgccc
     1021 atggaaaaaa acaatatcaa gaaagtcttt ataacaggag ctccgttggc aggattgaca
     1081 caagaaatac taaagccctt atccataatg aagaacatat ttgttgtttt atctcagtca
     1141 ttgtgcgaag gagaaaacta tcagcctata aatgaaggtg aaaaccctta tttgaattta
     1201 gcggaaaaat atatccggga tgatgatgaa atgaatgaat ttctaagggg caacgataca
     1261 ggtgccataa tagatgttaa actgtcagga tgtgatgtgc tgaaaattac tgaaatgaat
     1321 acttcattgc catctttatc gctgactgtt gattatgatt atgatataga aaaaattaat
     1381 aaagcattgc atttttttat ccataattag tgaagtatat gtttttttta taatatgccc
     1441 cttaaatata aaatatagta atttgatatc ctttacattt ttatttataa ttattatatg
     1501 ttacaaaata atacaaaatt tggaggatta aaatggcaga ttaccgtaaa atgtgggaag
     1561 acttaggaat ggatttagag ctgcatgatc tcttgtgtga tgcattaccc gtagcctttg
     1621 gggatgttta tatgtctatg gaaaaccgtc ctgaatcaat ggacttttat gattttgtcg
     1681 tatcggaaat tcatggtgtc agacctgcag aattagtaga attccagaaa aagggcggaa
     1741 aagtatttgg aacattctgt gtttatgtac cggatgaaat tgtatttgca gctaatggaa
     1801 ttgcaacggg tttatgcggc ggttcacaat tctgggtgcc aggcggagaa aaagttttac
     1861 ctacaaatac atgcgcctta ataaaggcat ctgttggtgc acgtttggaa agaacatgcc
     1921 cgtttttcag aattgcagat atatatgttg gtgaaactac atgtgacggt aagaaaaagg
     1981 cctgggagat cctggcggag gatgttcctg tacatattat ggacatgcct caaatgaaaa
     2041 gacctcagga tacagctaaa tggatagaag aaataaagat atttaaggat aaggttgaag
     2101 aagtaacagg aaatgaagta acagcagaaa aattatccga agcaattaaa ttgattaata
     2161 ataagagaaa agcattggct cgtctttatg aactgagaaa ggaaaaaaat attcctataa
     2221 gcggcaagga tgtgctgctt atatcacaaa tagctttcta tgacgacccc actcgtttta
     2281 ctcaaatgac aaataagctt tgtgatgaat tggatgaaag agtaaaaaat aacataagtg
     2341 tattccctga aggaacaaaa cgtattatga taacaggaac acctatggca atacctaact
     2401 ggaagatgca tcatatagtt gagacaagcg gcggtgctgt agtagttgaa gaagcatgca
     2461 ccggaacaag atattttgaa aacttggttg atgaaagcaa ggatacaatg gatgaacaaa
     2521 tagcagcttt ggcagacaga tacctgaaaa ataactgtgc ctgctttaca ccgaataccg
     2581 gaagaatcga tgatatttta agactggcta aggaatacaa agtagacggc ataattgacg
     2641 ttaatcttaa gttctgtaac ttgtatgaca ctgaaggtta ttttgttgaa aaggctttga
     2701 aagaagcagg tattccggta ttgggaattg aaaccgacta tactgaccag gatgctgagc
     2761 agctcagaac aagagttggt gcatttatag aaatgttgtc aaataaataa aatggagtac
     2821 tatatccttt ttaatgacgt tgacagtggt ttaaagcttt acaaccttct taaatcagct
     2881 aaaataaagg taactatttc acccactcca agagaggcaa gcaaatgctg cgggatttcc
     2941 ctgctcataa aaaacaagga cgacctgcct tcaatcaggg agtgcattga aaacaacaat
     3001 attgaaatta aagatatttt tcaaatggag tctaaaagag acccgaaaag agataaattt
     3061 tgttaaacaa taaagtgaca gagtgctcta tgcttatctg tcacttattt aatgcataaa
     3121 tcaaattcaa tctatatatt tattgtactt gattattttc taatataaat ataatataaa
     3181 tctatatatt gtaatggtgt catatagatt ataggcggag gaattcatct tttaagaatt
     3241 tctcttgagg ttgttgttat gttattcaca tccagaacac ggcaatgaca cagcaaaaca
     3301 tggttacata aaaatctaaa aaataatgaa aggatagatt aaatgaaata ttttataggg
     3361 gttccaatac ccaaaagcta taaaaataaa atagaaatgc tgagggctga gttcaggttc
     3421 ttaacaacgg agccccatat tactcttgtt cctcctccgg cacttcccga tgaggatgat
     3481 tttgttgaaa aggtagtgaa tgtgtgcaaa acaattgagc cttttgacat aagactggaa
     3541 gggcttgatc agttcgccac cagagtttta tatgtaagcg tcgattctcc gggtttaatt
     3601 gatttatata aaaaaatata tgaaaatctg ggcttgcagc aggaaagaag gggctttact
     3661 ccccacttaa cagttgtaaa acaaagacag gggcggcctg tggatataga ttcaataaga
     3721 aaaagggcag aaataaaact tattccttac cctgaatata ctctaaactc aattacagtc
     3781 tatcatcagc ccaaggaaca ttccatctat gttccctaca tggaaatacc ccttggaaaa
     3841 taaattttga ggacgaaaag aaccgtcccc aaaaaattat tttaggcgtt cttctatcag
     3901 ttttgccagc tgggcggcat ggaagtttat ttctt
//
LOCUS       DAOPUT010000120         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_25319_length_3700_cov_19.503155, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000120 DAOPUT010000000
VERSION     DAOPUT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25319_length_3700_cov_19.503155"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..457)
                     /locus_tag="PKK75_07870"
     CDS             complement(185..457)
                     /locus_tag="PKK75_07870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ83555.1"
                     /translation="MDFKYEKDKIYLENEKGECVAEVTFPLISKDKVNINHTYVNRIL
                     RGQGIADKLVRELAVYLRENNMKAVSTCSYAKDWFENHPEFHDVCK"
     gene            793..2460
                     /gene="hcp"
                     /locus_tag="PKK75_07875"
                     /gene_synonym="priS"
     CDS             793..2460
                     /gene="hcp"
                     /locus_tag="PKK75_07875"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNZ83556.1"
                     /translation="MSTQENLMFCWQCQETAGNKGCTKAGVCGKKPDTAGLQDLLIYT
                     SKGLSEVTTKLRNEGKEVSADVDHLITLNLFTTITNANFDNDVFKDRIKMTLKVKADL
                     TEKLSNKEGLSEAALWTGDADEFEVKASSTEVGVLSTANEDVRSLRELITYGLKGMSA
                     YMKHANVLGYDDKDVNIFMQATLAKLLDDSLTVDELVALTLETGKFGVSGMALLDRAN
                     TSTYGNPEITKVNIGVGKNPGILISGHDLGDLEQLLKQTEGTGVDVYTHSEMLPAHYY
                     PHLKKYKHLAGNYGNAWWKQKEEFESFNGPILMTTNCIVPPKDSYKDRLFTTGSAGYP
                     GCRHIEGTFGSEKDFSEIIELAKKCQAPTEIETGEIVGGFAHEQVFAVADKVVEAVKS
                     GAIKKFFVMAGCDGRAKSRNYYTDFAAALPKDTVILTAGCAKYKYNKLNLGDIGGIPR
                     VLDAGQCNDSYSLALIALKLKEVFELEDINDLPLAFNIAWYEQKAVIVLLALLYLGVK
                     NIHLGPTLPAFLSPNVANVLVENFGIAGIGTVEDDIKLFLRDSTVTA"
     gene            2510..2719
                     /locus_tag="PKK75_07880"
     CDS             2510..2719
                     /locus_tag="PKK75_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNZ83557.1"
                     /translation="MAKKVTKDMYIGEVLQIDPGLAGLLMEAGMHCLGCPSSQMESLE
                     DAAMVHGFNADELVNKLNAYLSSAK"
     gene            3012..>3700
                     /locus_tag="PKK75_07885"
     CDS             3012..>3700
                     /locus_tag="PKK75_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ83558.1"
                     /translation="MEKCNCNGHSCSHGYCIDNIPIFVDLPFEVKQSIMESSDHKVYK
                     KGEMIFSPGDCFDYLFVVNKGRVKLSKISVMGKEQILKILEVGDFAGELALFKDTVMT
                     NFAEAMEKTEICIIKNEKIHEIIVQRPEIALKFLEKYTERIQQSEEIIEQIGLREVEQ
                     RIANYLVSEVEKNNIKGKNKEYVINLPVTKSVLSSMLGTTKETLSRKLSLLQDGGLIR
                     LEGQRKIIITD"
BASE COUNT         1303 a          601 c          759 g         1037 t
ORIGIN      
        1 tttcatccat ttgctcaaag gtgtgttcaa tataggtttt tatgcatttc cacctgccat
       61 gcattctcta ttaaaaactt gtatatctac tattccttgt cacagcattt atcatttaaa
      121 atacattaac ataaattttt ataattgtaa agagctattt tcaaattttt ttcagctaaa
      181 attcttattt acatacatcg tggaattccg ggtggttttc aaaccaatct ttggcatagg
      241 agcatgttga cactgctttc atattatttt ctcttaaata tacagccagt tccctgacta
      301 atttatctgc gatgccttgc cctctcagga ttcggtttac ataagtatgg tttatgttaa
      361 ccttatcctt tgatataagt ggaaaagtta cttcagcaac acattctccc ttttcatttt
      421 ctaaatatat tttgtctttt tcatatttaa agtccatagt acctccggat taaatctaat
      481 cctaattttt cagcattctt atagaacaca ccttctgctt tgggatagtc tttaatggtc
      541 gaaggaatgt ccccaataga atattttagc atgtaagatg cttgaatctt attaaatcaa
      601 acaatatatt aattaatcca tcttgtcaac gcaaatatta atttgtttac agcctttata
      661 agagggtata agaataaagg atgataattt atactgcatt cgaatgtttg taacaaatgt
      721 tacggaaatg atttttgaaa tgcattaata ttaagtcaat aaataaatta aaaaagtcga
      781 aaggggaaca atatgagtac acaggaaaac ttgatgtttt gctggcagtg tcaggagact
      841 gcaggcaata aaggctgcac aaaggcgggg gtatgcggga aaaaacctga tacggcagga
      901 ttacaggact tgttaatcta tacatcaaag ggactgtctg aagtaaccac taaattaaga
      961 aatgaaggaa aagaagtatc agcagatgtt gatcacttaa taactttaaa tctatttaca
     1021 actattacaa atgcaaactt tgacaatgat gtttttaaag acagaattaa aatgacttta
     1081 aaagttaaag ccgatttgac agaaaaattg tcaaataaag aaggattgtc ggaagcagca
     1141 ctatggacgg gtgatgcaga tgagtttgaa gtaaaagcaa gttcaaccga agtgggcgtt
     1201 ttatccacag ccaatgaaga tgtaagaagc ttaagagaac ttataaccta cgggttaaag
     1261 ggtatgtcgg cgtatatgaa gcatgcaaat gtacttggct atgatgacaa agatgtaaat
     1321 atatttatgc aagccacact ggcaaaactg cttgatgatt cattgacagt tgatgaactg
     1381 gttgccttga cactggaaac aggaaaattc ggcgtaagcg gaatggcact gttagatagg
     1441 gcaaatacaa gcacttacgg aaacccggaa atcacaaagg taaatatcgg agtaggaaaa
     1501 aacccgggca tcttaatttc aggccatgac ttgggagacc tggagcaatt gttgaaacaa
     1561 acagaaggta caggagtgga tgtttacact cactcagaga tgctgccggc tcactactat
     1621 ccgcacttga agaaatataa acatttggca ggcaactacg gaaatgcctg gtggaaacaa
     1681 aaagaagaat ttgaatcatt caacggacct attctcatga ctacaaactg tattgtgcct
     1741 ccaaaggaca gctataagga cagattgttt acaaccggct cagcaggata tccgggatgc
     1801 aggcatattg aaggaacttt cggttcagaa aaagatttct ctgaaattat agaattagct
     1861 aaaaaatgtc aggctccaac ggaaatcgaa acaggcgaaa tagtgggtgg atttgcgcac
     1921 gagcaggtat ttgcagtagc tgacaaggtt gtcgaagcag ttaaatcagg agcaatcaag
     1981 aagttcttcg taatggcagg ctgcgatgga agagccaaat caaggaatta ctatacagac
     2041 tttgcagccg ccttgccaaa agacacggtt attttaacag cgggctgtgc aaaatataaa
     2101 tacaacaagc taaatttagg agatatagga ggaataccga gagttcttga tgcaggtcag
     2161 tgcaatgact catattccct cgccttaatt gctttaaaat taaaagaggt atttgagctt
     2221 gaagatataa atgatcttcc attagcattc aatattgcat ggtatgagca aaaagcagtt
     2281 atagtattgc tggctctcct atatttagga gtaaaaaata ttcatttggg accaacattg
     2341 cctgcattcc tatcaccgaa tgttgctaat gtgctggttg aaaactttgg catagcagga
     2401 atcggtactg tagaagacga cataaagctc tttttgaggg actcaacagt aactgcttaa
     2461 atgaatttaa attaatagat tggaaatgat aggagtatag gaggttaata tggcaaaaaa
     2521 agtaacaaag gacatgtata ttggagaagt attacagata gacccaggtt tggcaggact
     2581 attgatggaa gcaggaatgc actgtctcgg atgtccgtca tcacaaatgg aatcgcttga
     2641 agatgcagca atggtgcatg gtttcaatgc agatgagctt gttaacaagt taaatgctta
     2701 tttaagctca gcaaaataaa tttaacataa gagagcatgc cggcatgctc tcttattaag
     2761 aaagcagttc ggggatagtt accttaaagg tttgtcccca taccgttaaa accccttgat
     2821 gaagcaatag aagttacaaa tattcacggg tggattcagg aaagagtttc acaagaacta
     2881 ttacagctta agagatgagt ttataaaagg aagaaaaatt tctttttaag gcggctgaag
     2941 aaaaaggaac tcaaaaatac tatctggacc tcctaagaaa attagaagaa aaatatcaat
     3001 aggaggagca catggaaaaa tgcaattgca acggccacag ctgcagccat ggatattgta
     3061 tagataatat tcccattttt gtggacttgc cctttgaagt aaaacaaagc attatggaat
     3121 catccgatca taaggtttat aaaaaaggcg agatgatatt cagccctgga gattgctttg
     3181 attacttatt cgtagttaat aaaggaagag ttaagttatc gaaaatctct gtcatgggca
     3241 aggaacaaat actaaaaatt cttgaagtcg gagatttcgc cggtgaacta gccctcttta
     3301 aagatactgt gatgactaat tttgctgaag ctatggaaaa aactgaaatc tgcataataa
     3361 aaaatgaaaa aattcatgaa attatagtgc aaaggcctga aatcgccctt aagtttctgg
     3421 aaaaatacac tgaaagaatt caacagtcgg aggagattat agagcagata ggccttaggg
     3481 aagtggaaca aaggattgcc aattacctgg tttcagaagt agaaaaaaac aatattaaag
     3541 gcaagaataa ggaatatgta ataaacctgc cggtaaccaa aagcgtcttg tcttccatgc
     3601 tgggaacaac aaaagaaacc ctcagccgga aactatctct cctgcaggat ggaggtctta
     3661 taaggcttga aggtcaaaga aaaattatta ttacagatat
//
LOCUS       DAOPUT010000121         3553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_26771_length_3553_cov_20.020011, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000121 DAOPUT010000000
VERSION     DAOPUT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26771_length_3553_cov_20.020011"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PKK75_07890"
     CDS             complement(<1..854)
                     /locus_tag="PKK75_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNZ83559.1"
                     /translation="MELKNFRDLIEKVQKCESRKRVAVAAAQDEHTLEAVFRAAKDKL
                     VEPLLVGNKEKIEEILKELDVDYDISSIISTESDKESAEKTVELINEGKADFIMKGKL
                     QTADLLRAVVDKEKGLRTGRVMSHVAILEVPAYHKLIAVTDGGMMMYPNADEKKQILE
                     SAVDVFLAMGYECPKVAVLAAVETVNPKMPEAVDADILKKMNQSEEIKDCIVEGPISF
                     DLTFSKESAEIKGYSSPVTGEADILLAPNITTGNIMSKALIEMAGATMAGMIVGAKVP
                     IVLTSRGAS"
     gene            complement(1041..2876)
                     /locus_tag="PKK75_07895"
     CDS             complement(1041..2876)
                     /locus_tag="PKK75_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNZ83560.1"
                     /translation="MKKLAGRLIVSAIFFVAAIIINDSQLKLIMYAISYISAGGDVVK
                     RAVYNIKDGEVFDENFLMSLASIGAFLIGEAPEGVAVMLFYQVGELFQNYAVGQSRRS
                     IAALMDIRPDYANVFRNGGVVTVSPEEVRIGEIIQVKAGERVPLDGIVTEGSSILDTS
                     ALTGESLPREVIAGSELLSGCININGLIKAKVTKLYSESTVSKILELVENAGSRKSKS
                     EKFITKFARYYTPVVVVIAAILAIIPPLVIKEASFSEWIYRSLAFLVVSCPCALVISV
                     PLSFFGGIGGASKNGILVKGSNYLEAMAKTEVVVLDKTGTLTKGVFKVQKIHSVRDKP
                     EDLLKIAAYAENYSNHPISLSLKDAYKKEIETGKISHVEEISGQGVSAVVENIKVLAG
                     NDKLMEKFNIPYEKEASEGTVVHVAQDGEYAGYIVIADQIKDDAVEAMKALKQQNIKD
                     IVMLTGDSESAGLNTARELGIDKVHARLLPADKVQKVEELISHKSGRGTLMFAGDGIN
                     DAPVLARADVGVAMGALGSDAAIEAADVVIMTDEPSKIAAAIKISKNTLKIAYQNIIF
                     AISVKVLILILSTFGLTSLWAAVFADVGVAFIAVLNAMRALSFKN"
     gene            complement(2889..3260)
                     /locus_tag="PKK75_07900"
     CDS             complement(2889..3260)
                     /locus_tag="PKK75_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNZ83561.1"
                     /translation="MSVNDELICDCEVIHGDIVNEVKKRMPEDEDIYDLSDFFKVLGD
                     STRMRIIWALDESEMCVCDIAVLLNMTKSAISHQLRALRNANLVKFRREGKTVFYALK
                     DKHVRDIYEIGMEHIKEKYNA"
BASE COUNT          961 a          860 c          573 g         1159 t
ORIGIN      
        1 gatgctcctc ttgatgtcag gactatgggc acctttgctc caactatcat ccctgccata
       61 gttgcgcctg ccatttcaat caatgccttg ctcataatat ttccagttgt tatgtttggt
      121 gccagcaaaa tgtcagcttc tcccgttacc gggctggaat agcctttaat ctctgctgat
      181 tctttgctga atgtcaaatc aaatgaaata ggtccttcca ctatgcaatc cttgatttct
      241 tctgactgat tcattttctt aagaatatcc gcatcaacgg cttccggcat tttgggattt
      301 acggtttcta ccgctgcaag cacagcaacc ttagggcatt cataccccat tgccagaaaa
      361 acatcaactg cactttcaag tatctgtttc ttttcatcag cattaggata catcatcata
      421 ccgccgtcag ttacagctat aagcttgtgg taagccggaa cttccaaaat tgcaacatgg
      481 gacatgaccc tgccggttct taggcccttc tccttgtcta caaccgccct cagcaaatct
      541 gccgtctgca gctttccctt cattatgaag tctgccttac cttcatttat taattcaacg
      601 gttttttctg cagactcctt gtcgctttcc gtgctgataa tactgcttat gtcataatca
      661 acatcaagtt cttttaatat ttcttcaatt ttttctttat ttcctactag taaaggttca
      721 accaacttgt cctttgctgc tctgaagact gcctccaatg tatgttcatc ctgggctgcg
      781 gcaacagcaa cccgttttct tgattcgcat ttttgaacct tttcaatcaa atcccggaaa
      841 tttttaagct ccatgtatta cctccttata taagtacatt tgaaaattta ggggtattcc
      901 cttcatggta accctcatag ggtgacttcg caagatgcgt tccccttcct tgataacaat
      961 tataccatta ttcccaaaga cttcaataga atttcacaag gggacgtata tgacaaggtg
     1021 acaggggttc tgtcatataa ttaattttta aaactcagag ccctcatggc attgagcaca
     1081 gcaataaatg ctactcccac atctgcaaat acagctgccc ataggctggt cagcccgaag
     1141 gtgcttagaa tcaaaattaa tacttttaca gatatggcaa aaataatatt ttgatatgct
     1201 atctttaatg tatttttaga aattttaatg gctgctgcta tttttgacgg ttcgtctgtc
     1261 attattacaa catctgcagc ttcaattgcg gcatccgatc caagcgcccc cattgcaaca
     1321 cccacatcag ctcttgccaa aacaggagca tcattaatgc cgtctccggc aaacatcaaa
     1381 gttcccctcc cggatttatg agaaattaat tcctccacct tttgaacctt gtctgcaggc
     1441 aggaggcggg catgaacctt atctattccc aattcccttg cagtatttaa acctgctgac
     1501 tcactgtcgc ccgtaagcat tacaatatcc ttaatatttt gttgcttcaa tgccttcatt
     1561 gcctccacag catcatcctt aatctgatcg gcaattacaa tatacccagc atattctccg
     1621 tcttgagcaa catggacgac agttccttct gatgcttctt tctcatatgg aatattgaac
     1681 ttttccataa gcttgtcatt gccggctaaa acttttatat tttccactac tgcagatacg
     1741 ccctgtcccg aaatctcttc tacatgggaa atcttgccgg tttcaatctc ctttttatat
     1801 gcatccttta aagaaagtga aataggatga tttgaataat tttctgcata agctgcaatt
     1861 tttagcaggt cttcaggctt atccctgacg gaatgtattt tttgtacttt gaatacacct
     1921 tttgtcaagg tccctgtttt gtcaagcacc actacttctg ttttagccat ggcttccagg
     1981 taattgcttc cttttacaag aattccgttt ttggaagcac ctcctatccc cccgaaaaaa
     2041 ctcaggggaa ctgaaatcac cagtgcacaa ggacaggaca caaccaaaaa tgccaatgat
     2101 ctgtaaatcc attcactaaa tgacgcctcc tttataacaa gaggtggtat tattgcaaga
     2161 atggctgcaa ttacaacaac tacgggagtg taatacctgg cgaatttagt tataaatttc
     2221 tctgatttgg atttcctgct gccggcattt tccaccaatt caagtatctt gctgacggtg
     2281 gattcgctgt agagctttgt aaccttcgcc tttatcaatc cgttaatgtt gatgcagccg
     2341 ctcaaaagct cacttccggc tataacttcc cgaggcaggg attctcctgt taatgctgat
     2401 gtgtcaagta ttgagcttcc ttctgtgaca atgccgtcaa gaggaaccct ttctcctgct
     2461 tttacctgaa taatttcacc tatccggacc tcttcgggag atactgtaac taccccgccg
     2521 tttctaaata catttgcata atcagggcgg atatccatca aagctgcaat cgaccttcgg
     2581 gattgcccca cggcatagtt ttgaaacaat tctccaacct ggtaaaacag cattacggca
     2641 acgccttcgg gagcttctcc aatcaagaat gctccgattg aagcaagaga catcaaaaag
     2701 ttttcatcaa aaacttctcc gtctttaatg ttgtaaacag ccctttttac tacatcgccc
     2761 ccggctgata tatagcttat tgcatacatt attaatttta attgactgtc atttattatt
     2821 attgcagcaa caaaaaatat tgcactgact attaagcgcc ctgctaactt tttcatttga
     2881 ttctcctatt atgcgttata tttttctttg atatgctcca tgccgatttc gtaaatatct
     2941 ctaacatgct tatccttcag cgcgtaaaaa accgtcttgc cttctcttct gaattttacc
     3001 aaatttgcat ttcttaatgc cctaagctgg tgcgaaatgg ctgatttggt catattcaac
     3061 aggacagcta tgtcacacac gcacatttca ctttcatcca gagcccatat tattctcatt
     3121 cttgtactgt ctcccaatac tttaaagaag tctgacaaat cataaatatc ttcgtcctca
     3181 ggcattcttt tttttacttc attaacaata tctccatgaa taacttcaca atcacaaatc
     3241 agttcatcat ttacagacat tttaacctcc caatcagttg agcagttgtt caaccgtttg
     3301 attatattat agttgaacaa ctgctcaatt gtcaatagca ttattaaaaa acgacaacaa
     3361 attatttcaa tatcgtatat atagatgtct gtaaaatatt ttgaatataa gagcacaaaa
     3421 aagtcattcc aagcacattt caagtagctc cctatgctcg gatgactata ttgctgcctg
     3481 gtataactat ggtccctctg aatccgttcc atcagaaaaa tccaggattt cagactggga
     3541 gagaattgct ccc
//
LOCUS       DAOPUT010000122         3536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_26954_length_3536_cov_17.775639, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000122 DAOPUT010000000
VERSION     DAOPUT010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26954_length_3536_cov_17.775639"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..481)
                     /locus_tag="PKK75_07905"
     CDS             complement(101..481)
                     /locus_tag="PKK75_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNZ83562.1"
                     /translation="MNNVIQTSNAPAAVGPYSQGIKAGNTIYVSGQLAFNPATGELYK
                     GGDIKEETRMCLMNVKAILEAGGASLKDVVKCTVYIKDMNQFGLINEAYAQVFQDTKP
                     ARACIEVARLPRDGQVEIEAIAVV"
     gene            complement(491..859)
                     /gene="rsfS"
                     /locus_tag="PKK75_07910"
     CDS             complement(491..859)
                     /gene="rsfS"
                     /locus_tag="PKK75_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNZ83563.1"
                     /translation="MSSVQKSIETILKACDNKKAYNFIVLNVSKSSSITDYFIICSGN
                     NEKQTVAIAEEVLEKGALAGLDFFYKEGFETGRWILLETDDIIVHIFHKDERAIYDLE
                     SLWYDNNSLDVEQYGIKNWK"
     gene            complement(856..1356)
                     /gene="yqeK"
                     /locus_tag="PKK75_07915"
     CDS             complement(856..1356)
                     /gene="yqeK"
                     /locus_tag="PKK75_07915"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HNZ83564.1"
                     /translation="MQIEEIKKELEIELGKKLYLHSIGTMEEAEKLASVHGCDKSKAK
                     IAGLLHDCWKGRCKDNLKHSKESAELAKTKFDVKDDDILNAIMYHTTGRVNMTLLEKI
                     IFIADKIEPNRHYPGVEEIRREAYINLDEAIIKSLESTIEYVKNRNLVLDMESVNTLN
                     YLKEGK"
     gene            complement(1358..1966)
                     /gene="nadD"
                     /locus_tag="PKK75_07920"
     CDS             complement(1358..1966)
                     /gene="nadD"
                     /locus_tag="PKK75_07920"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNZ83565.1"
                     /translation="MKRKVGIMGGSFDPIHIGHLVVANEALNIYELDEIIFVPAGDPP
                     HKSSLKADPFHRLFMVNMAVLSNDKFSVSDLEIKCRGKSYTLNTLREFHKMYEDTEFY
                     FITGTDAVIDLPNWYKPEEVLKLCRFIAASRPGISIKEVNEKIDEIKKTFGGNIELLK
                     IPMLQISSTEIRKRVSMGISVKYLLPESVEQYIIKNKLYKEK"
     gene            complement(1963..3396)
                     /locus_tag="PKK75_07925"
     CDS             complement(1963..3396)
                     /locus_tag="PKK75_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HNZ83566.1"
                     /translation="MAFSDYVLFSVFYFWASSTALSIGIGYYTIYRPILAGLAAGLIL
                     NDVQTGMMAGAVVNIIYIDFVSTGGSFKGDQCLTALMAAVASIAFGLHPLEAAALAFP
                     FGFLGILIWKYRLRINNTFVHMYEKKYKENKAPDISLYDGLFPQLLLLFMSSAVITAG
                     LLLMFSFNRLMNFKSISALYYIGLILIIFSSFNILYKIKNKYNFIIFAVVFLAAAITE
                     IRAYIMLILIALILLFTSNKKINIVRNKLKGSIKKGDLVYSWFIWMNYSHSCYSYERL
                     MGLAFAHSMKNIIKKLYKDKHEIAEAVHNHTEFFNTEPNMGTPILGYIISLEEERKIS
                     RESFENISYIKKGMMGISAGLGDSFTQVVLTPLFVSMAVMLCLDKSYNLAFIPVIYLA
                     AYILFISYNGFMNGYFQGKDSMLKRVRNVKESRLKKYFPCMFSGILGLSMSSLLFNNI
                     RPLENLFTLVIILLAAGLTFLRRRREQ"
BASE COUNT         1063 a          727 c          530 g         1216 t
ORIGIN      
        1 aggaaaaaac ggcgaagcag gtacaataat cagtaataaa aaagttgcag gatttatgca
       61 actttttatt atcacgttta cattccagtt acggtagaca ttacacaact gctattgctt
      121 caatttccac ttgtccgtcc ctggggagtc ttgctacttc aatacatgcc cttgcaggtt
      181 tagtatcctg gaacacttga gcatatgctt cgtttatcag cccgaattgg ttcatatcct
      241 tgatatatac ggtacattta acaacatcct taagagatgc tcctcctgcc tccaaaatag
      301 ctttaacatt cattaagcac atcctggttt cttccttaat gtcaccgcct ttgtaaagtt
      361 cccctgttgc cggattaaat gcaagctgtc cggaaacata tattgtattt cctgctttaa
      421 ttccttgaga ataaggccct actgcagcag gtgcattgct tgtttgaata acattattca
      481 taataccctc ctatttccaa ttttttattc catattgttc aacatccaat gaattgttat
      541 cataccacaa tgattccagg tcataaattg ccctttcatc cttatgaaag atatgaacaa
      601 taatgtcatc agtttctaat aaaatccatc ttccggtttc aaaaccttcc ttgtagaaaa
      661 aatctaaccc tgccaaggca cccttttcaa gtacctcttc agcaattgca actgtctgtt
      721 tttcgttatt accgctgcat atgatgaaat aatcagtaat tgagctggat ttggacacat
      781 ttaacacaat gaaattataa gcctttttat tgtcgcaggc ttttaaaatt gtttcgatac
      841 ttttttggac tgaactcatt taccctcctt taaatagttt agtgtattta ctgattccat
      901 atcaagaacc aaatttctgt tttttacata ttcaatagta ctttccaacg acttaattat
      961 tgcttcatca agatttatat atgcttctct tctgatttcc tcaaccccgg gataatgcct
     1021 gttgggctca attttatctg ctataaatat tattttttcc aaaagagtca tatttactct
     1081 tccggttgtg tgatacatta ttgcattcaa tatatcatca tctttaacat caaactttgt
     1141 ctttgccagc tctgcgcttt cttttgaatg ctttaaattg tctttgcatc ttcccttcca
     1201 gcagtcatgc aataagcctg caatttttgc cttacttttg tcacagccat ggactgatgc
     1261 aagtttttcg gcttcctcca ttgttccgat gctgtggagg tacaattttt tacctaattc
     1321 aatttccaat tcctttttta tttcttcaat ttgcatttca tttttcctta tacaacttat
     1381 tctttattat atattgttcg acactttcag gcaataagta ttttacagat attcccatgg
     1441 agactctttt cctgatttcc gttgaagata tttgaagcat gggaatcttt aataattcta
     1501 tattcccgcc gaaggttttt tttatttcat caatcttttc attaacttcc ttaatactta
     1561 ttcccggtct tgaagctgca ataaatctgc aaagcttcag tacctcttca ggtttatacc
     1621 agtttggaag gtctatcacc gcatcagttc ccgttatgaa ataaaactcc gtatcttcat
     1681 acatcttgtg aaattccctt aaagtattca aggtatagct tttgcccctg cattttattt
     1741 caaggtctga gacactgaat ttgtcattgg acaatactgc catattaacc ataaacaatc
     1801 tgtgaaatgg atcagcctta aggcttgatt tatgtggcgg gtcaccggca gggacaaaaa
     1861 ttatttcatc cagttcatat atatttaatg cttcatttgc tacaaccaag tggcctatgt
     1921 gaatagggtc aaaagagccg cccattattc ccacttttct tttcattgtt ctctccttct
     1981 tcttaaaaaa gttaaaccgg ctgcaagtaa aattatgact aatgtaaata aattttccag
     2041 aggtcttatg ttgttaaaaa gcaggctgct cattgataag cctaaaattc ctgaaaacat
     2101 acagggaaaa tattttttga gcctgctctc ttttacattc cttactctct taagcatact
     2161 gtctttaccc tgaaaataac cattcataaa accgttgtag gatatgaaaa gtatgtaggc
     2221 tgcaagataa attaccggta taaatgccaa attatagctc ttgtccaggc aaagcatcac
     2281 agccatagac acaaacaggg gggttaaaac cacctgggtg aaggaatcac ccaatcccgc
     2341 cgatataccc atcattccct tttttatgta ggaaatattc tcaaagcttt ccctgcttat
     2401 ttttctttct tcttccaggg atattatata tcccagtatc ggagtgccca tattaggctc
     2461 agtattgaaa aattctgtat gattgtggac agcttctgct atctcatgtt tatctttata
     2521 caatttttta ataatatttt tcatgctgtg ggcaaaagcc aaacccataa gtctttcata
     2581 gctgtagcat gaatgggaat aattcatcca tataaaccat gaataaacca agtctccctt
     2641 tttaatactg cccttaagtt tatttcttac aatatttatt ttcttatttg acgtaaacaa
     2701 caaaataagt gctatgagaa taagcattat atatgctctg atttcagtaa ttgcagctgc
     2761 caaaaaaaca acagcgaaaa taataaaatt gtatttattc tttattttgt ataaaatatt
     2821 aaaagaagaa aatatgatca gtatgagtcc tatataataa agtgcactta tacttttaaa
     2881 attcattaat ctgttgaatg aaaacatcag caacaagccg gctgttataa ctgcagaact
     2941 cataaacaaa agtagaagct gaggaaaaag accgtcataa agacttatat cgggagcttt
     3001 gttctcttta tacttctttt catacatatg tacaaaagta ttgtttattc tcaatctgta
     3061 tttccatatc aatatcccta aaaatccaaa gggaaatgca agagctgcag cctcaagagg
     3121 atgaaggcca aatgctatgg aggcaacagc cgccataagg gcagtcaggc attggtcccc
     3181 tttaaatgag cccccggtgg aaacaaagtc aatataaata atattgacaa ccgcacctgc
     3241 catcatgcca gtttgtacat cattaagaat aaggcccgca gccagcccgg ccaatatggg
     3301 tctgtaaatc gtgtaataac cgatgcctat ggaaagtgcg gtactgcttg cccaaaaata
     3361 gaaaacgcta aataatacat aatcagaaaa agccatatat ctttacctta taacaagagc
     3421 cttatccctt ggaaggattc ttacctcgac attaacattt tcctttgcca tatcctttaa
     3481 aatatttaac tcatggtcgc ttaaatgaat ggagttgtag tatctttttc ttcctt
//
LOCUS       DAOPUT010000123         3526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_27060_length_3526_cov_17.206569, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000123 DAOPUT010000000
VERSION     DAOPUT010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27060_length_3526_cov_17.206569"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1607
                     /locus_tag="PKK75_07930"
     CDS             21..1607
                     /locus_tag="PKK75_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ83567.1"
                     /translation="MVTSNYQKEFDAVVIGAGNGGLTAAATLAGKGMKTLLLEQHNLP
                     GGFATSFVRGRFEFEPSLHEISDFGPPEDKGNVRQLLEDKIGLDLQWIRVPEAYRLII
                     PNEKGGRLDVVMPFGKEEYLSKMEEYVPGSRHSVTEFIRLCEEVVNAFSYLAESKGNA
                     DKNVLTDKYSNFLKTAPYSLQEVLDALKIPYLAQKILTAYWCYIGLGTNRMNFTIFAA
                     MFYRYITRAAYIPKYRSHGYTVALDKKIRELGGEIQYNTKVEKILVDDGKVVGIETTR
                     GDRIKTNYIVSNASPHLVYNQLIYPKNEVPEIALKTCNARKVGFSAFVVYLGLNKSPE
                     ELGINEYSYFVYSTPDTNDIYKQFNKLERPTGQATVCLNNSIPDCSPEGTSIVYLTTL
                     YSGDSWSNVESKDYFKLKNKIAEEMIDEFEKAVGINIRDSIEEIEVATPATFANYTGA
                     FNGSVYGYEPVAWDSVLPRMMCMNDEKYIKGLEFAGGFAFRCHGYSSSLQSGETAALL
                     TYRDYMEVKNNEQRKIEHQS"
     assembly_gap    1645..1744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1745..2650
                     /locus_tag="PKK75_07935"
     CDS             <1745..2650
                     /locus_tag="PKK75_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HNZ83568.1"
                     /translation="TVKRPYSISSTPQEALEGYYDITVKKGEGGFVTSYIWDNWHTGT
                     KIVSGGPEGHFYYNNLRDSKKLIGIAGGCGITPFRSIAKSIVEGSIDAELTLFYGCNK
                     KDDLIFYNEFNELEKNSKGRFKVVYVLAEEDIEGFEQGFITSDLISRHTDIENSSFFI
                     CGPQGLYNFISKELSNLRIVRKLIRREVFGEIKDIGKYAGFPDEVSEKTFNVKVHMGD
                     SFTTIPAMPKESLLVAMERAGLCPPASCRSGSCGVCRSLLIDGNVFIPEDVDGRRHAD
                     IQFGYIHPCSTFPTGDIEMIVPRES"
     gene            2754..3470
                     /locus_tag="PKK75_07940"
     CDS             2754..3470
                     /locus_tag="PKK75_07940"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNZ83569.1"
                     /translation="MNSDYNTLKAESLKELFVKEIEAKIISGQLKPGDRLPPEREMAS
                     LMGISRSVVNSGILDLASKGFVEIIPRIGTFIVDYKKEGTPLILSSIMNYNEGKISLK
                     LFHSLIDTRLLIEVESAGLAAVNRTDDDLKSLEKLIIDVKENLDDVESFVEFNYKFHH
                     QVTIASGNIVYGMIFKSFEPVCKNLIRMYFKEGNYSVHSLASHEKLYQAIKDRNKDMA
                     EKIMKEILLEGSEELTRLLS"
BASE COUNT         1114 a          600 c          751 g          961 t
ORIGIN      
        1 ataaggagag gagtgattat atggttacga gtaactacca gaaggaattt gatgctgtag
       61 taataggagc cggcaacggc ggacttactg ctgcagctac cctggcaggc aaagggatga
      121 agactttact tttagaacag cataatctgc ctggtggttt tgcaacaagc tttgtaaggg
      181 ggaggtttga atttgagcct tcgcttcatg aaatctctga tttcggcccc ccggaagaca
      241 aaggcaacgt aagacagctc ttggaagaca aaataggtct ggaccttcag tggataaggg
      301 ttccggaggc ctacagactt attatcccaa atgaaaaggg tggcagactg gatgtggtta
      361 tgccctttgg caaagaagag tacctttcaa aaatggagga atatgttccc ggttcaagac
      421 actcagtaac tgaatttatt agattatgcg aggaagtggt taatgctttc tcctaccttg
      481 ctgaatcaaa aggaaatgcc gataaaaatg ttttgacaga taagtattct aatttcctta
      541 agactgcccc atattcactt caagaagttt tagacgcatt gaaaattccc tacctggcac
      601 aaaaaattct tacagcttac tggtgttata tcggtttagg gactaatcga atgaatttta
      661 ctatttttgc agctatgttt tacagatata ttacccgtgc cgcttacata ccaaaatatc
      721 gttctcatgg atatacagtt gctcttgata aaaaaatcag ggaattaggc ggagaaatcc
      781 agtataatac caaggtggaa aaaatcctgg ttgatgatgg taaagttgta ggtattgaaa
      841 ccacaagagg tgacagaata aaaacaaatt atatagtttc taatgcttca ccccatttag
      901 tatacaacca gcttatctac cccaaaaatg aggtgcctga aattgcacta aagacatgca
      961 atgccagaaa ggtaggtttt tctgcatttg tagtatattt aggtctcaat aagagtcctg
     1021 aagaactggg tatcaatgaa tacagctatt ttgtttacag cacacctgat acaaatgata
     1081 tctataaaca gtttaataaa ctggaacgac caacgggaca agctacagtc tgcttaaata
     1141 attcaattcc cgactgctcc cctgaaggaa caagcattgt gtatctgaca actctctatt
     1201 ccggtgacag ctggagtaat gttgagtcta aggattattt caaacttaag aataaaatag
     1261 cggaagaaat gattgatgag tttgaaaaag cagtgggcat caatataaga gacagcatag
     1321 aagaaataga agtagcaact cctgcaacct ttgccaatta tacaggcgct ttcaacggga
     1381 gtgtctacgg ttacgagcct gtggcctggg actctgtact gccaagaatg atgtgcatga
     1441 atgatgaaaa atatataaaa ggccttgaat ttgccggagg ctttgctttc agatgccatg
     1501 gctactcaag ctcgcttcag tccggtgaaa cagccgcttt acttacttac agggattata
     1561 tggaggtgaa aaataatgaa caaagaaaaa ttgaacatca aagttaagcc cttgggtatt
     1621 ttggatttgc ttgcatttaa aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnactgtt aaaaggcctt actctatatc ctctactcct caggaagctt tagaagggta
     1801 ttatgatatt acagttaaaa aaggcgaggg cggatttgtt acctcttata tctgggataa
     1861 ctggcacaca ggtacaaaaa tagtatccgg cggtcctgaa ggacatttct attataataa
     1921 tttaagagat tcaaaaaaat taataggaat agccggaggc tgcggcataa cacccttcag
     1981 gtccatcgcc aaaagtattg ttgaaggttc tattgatgca gagctcacac ttttttacgg
     2041 gtgcaacaaa aaagacgatt taattttcta caatgaattc aatgagcttg agaaaaactc
     2101 caagggcaga tttaaggtcg tgtatgttct tgccgaggag gatatcgaag gatttgagca
     2161 gggatttata acatcagacc ttataagccg ccatacggat atagaaaaca gctcgttttt
     2221 catatgcggt ccccagggtt tgtacaactt catcagtaaa gagctttcaa atctgagaat
     2281 tgtaagaaag ctcatccgca gggaggtctt tggagagatt aaggatattg gaaaatatgc
     2341 cggttttcct gatgaagtat ccgaaaagac ctttaatgtc aaagttcata tgggagacag
     2401 tttcacaaca atacctgcca tgccaaaaga atcccttttg gttgccatgg aaagagccgg
     2461 gctttgcccc cctgccagct gcaggagcgg gtcctgcggt gtctgcagat cgcttttaat
     2521 cgacggtaat gtatttatac cggaagatgt tgacggcaga agacatgctg acattcaatt
     2581 tggttatata cacccttgct caaccttccc gacaggtgat attgaaatga ttgtgcccag
     2641 ggaaagttaa ggtatgccga gagaaaatta aggtgtgcct tcaacgtctt gacattggca
     2701 gtctgaaatt ataatattat atgtagaaat ttactcgaag ttggaggctg aaattgaact
     2761 cagactataa tactctcaaa gcagaaagct taaaggaatt gtttgtaaaa gaaattgaag
     2821 caaagattat ttcaggacaa ctcaagccgg gagacaggct tccccctgaa cgggaaatgg
     2881 cttcacttat ggggataagc cgaagtgttg taaattccgg tatccttgat ctggcatcaa
     2941 agggctttgt agagattata cccaggatag gcacatttat agtagattat aaaaaggaag
     3001 gaactcctct tatcctttct tccataatga attacaacga aggcaaaatc agtctgaaat
     3061 tatttcatag cttgattgat actaggcttt tgattgaagt ggagagtgcc ggacttgcag
     3121 cagtgaatag aacagatgat gatttgaaaa gtcttgaaaa gttaataatt gatgtaaaag
     3181 aaaaccttga tgatgtggaa agttttgttg aattcaatta caaattccat caccaggtta
     3241 ccatagcttc cggtaatatt gtctatggga tgatatttaa atcctttgag ccggtatgta
     3301 aaaacctgat caggatgtat tttaaagaag gaaactattc cgtccattcc ttggcctccc
     3361 atgaaaagct ttatcaagca ataaaggata gaaacaagga tatggcagaa aaaatcatga
     3421 aggagattct tctggaaggc agcgaggaat taacaagatt gctgagctga atttttaccc
     3481 gcctgatttt gtttttatat gatatattaa tcagatatga tataat
//
LOCUS       DAOPUT010000124         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_28533_length_3388_cov_14.777978, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000124 DAOPUT010000000
VERSION     DAOPUT010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28533_length_3388_cov_14.777978"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1921)
                     /locus_tag="PKK75_07945"
     CDS             complement(35..1921)
                     /locus_tag="PKK75_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNZ83570.1"
                     /translation="MYGYAGTIAKINLTTGEISDYPLKEEDRILYLGGKSLAAKIIYD
                     SLKEKTEAFSEENLIVITTSPLTGTGSPSSSRFNVSAISPLTELLASSNCGGTFGLKL
                     KSAGYDGLVISGKSKEKVYIEISENRIEIKDASHLWGKTTGQTQDMLGNRSVGKFVIG
                     PAGENMVRYAAVISDERAAGRCGVGAVFGYKNLKAITAAGTKRTEIHDDKKFKAHNQR
                     WIGSLKKHIMTGRQIPELGTASFVNPMNKHNLLATKNYKSGSYDNHEKICGETLRNDY
                     LVKNKGCVTCPIQCGRVVKVSGKEVKGPELETITLLGSNLLNGSLENIIELNYLCDEY
                     GIDTMSFGSSIGLAMELNEKGMWNNGINFGDSTKLVQLVKMTAYREGIGNELAEGTRR
                     MSEKFGGRDFAINVKGLELAAYEPRGAQGMGLGYATSNRGGCHLNGGYMVVLEGLVLN
                     TNSKTTKGKAAFTIFFQDLMEAISAGGSCLFTSISVVPDFLIKNPENLIVKLINKMLP
                     LFGGIVGFMHNHSRLLGFNLKNIVAYPYSIKLVTGFDMNLGKFIQAGERSYNLERIIN
                     IRQGLTGRDDTLPKRLTEEFQLSYDPNSKVKLNEMLKKYYKIRGWDDKGIPTIDRMNK
                     LNIH"
     gene            complement(1924..3126)
                     /locus_tag="PKK75_07950"
     CDS             complement(1924..3126)
                     /locus_tag="PKK75_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNZ83571.1"
                     /translation="MFEVFCRIYQFIMKISANFMPFRVPELLEGENSLKKLPGTIKEK
                     GISNVLIVTGSHISSMGLINNLLEGLNEKNIKYAIYNKTTPNPTIDNIEEALLVYKQN
                     KCEGIVAFGGGSPMDCAKGIAARVARPNKTIPQMKGLLKVHKRIPFLFAVPTTAGTGS
                     ETTVAAVVRDSTTNEKYSITDMSLIPHVAVLDPALTLNLPPHITASTGMDALCHAVEA
                     YIGRSNTKDTYAYARKAIKLIFDNLYEAYSNGSNIEARAKMQKASFYAGLAFTRAYVG
                     YVHAIAHTLGGFYSMPHGLANAVIMPYVFEFYGNNAGKALAELADLIEIGEINDSQEE
                     KTGKFIDAIKNLNKSMDIPDKISGIKEEDIPTMAQRAFKEANPLYPVPRIMTKEDIIS
                     IYYKIKEG"
BASE COUNT          862 a          745 c          618 g         1163 t
ORIGIN      
        1 accgtcccct ttggacttac cgtccccttt ggacttaatg tatgttaagt ttattcattc
       61 tatctatggt tgggatgccc ttgtcatccc agcctcttat tttatagtat ttcttaagca
      121 tttcatttaa tttaactttg ctgtttgggt catagctgag ttgaaattct tcagtaagac
      181 gttttggaag ggtgtcgtct cttcccgtga gaccttgcct tatgtttatt atcctttcaa
      241 gattatagct tctttctcct gcctggatga acttgcccag attcatatca aaaccggtaa
      301 ccagcttgat ggaatatggg tatgcaacaa tgtttttcag gttaaagccg agaagcctgc
      361 tgtgattatg cataaatcca actattcctc caaacagagg aagcatttta ttaatgagct
      421 ttacaatcag gttttcgggg ttctttatta aaaaatcggg aaccacagaa atactggtaa
      481 acaaacatga gcctccggct gaaattgctt ccattaagtc ctgaaaaaat attgtaaagg
      541 cagcttttcc ttttgtagtt ttactgtttg tattaagcac taggccttcc aagacaacca
      601 tgtaaccgcc attcaggtgg catccgcccc tgttggatgt agcataacca agtcccatgc
      661 cctgtgcgcc ccggggctca taagccgcca gttccagacc ttttacatta attgcgaaat
      721 ccctgccccc gaatttttcg gacatgcgcc tggttccttc agctaattcg tttccgatac
      781 cttcccgata agccgtcatt ttgactaatt gtacaagttt tgttgagtct ccaaaattaa
      841 tgccgttatt ccacattcct ttttcgttta attccattgc cagaccgatg ctgcttccaa
      901 agctcatggt atctatgccg tactcatcac atagatagtt aagctcaatt atgttttcaa
      961 ggctgccatt gaggagattg ctccctagaa gagtgattgt ctcaagctca ggccctttta
     1021 cttctttgcc tgatactttt acaactctcc cgcattgaat cgggcatgta acacagcctt
     1081 tatttttgac aagataatca tttctcaatg tttcgccgca tattttctca tgattatcgt
     1141 agctcccgga tttataattt tttgtggcaa gaagattatg tttgttcatg ggattcacaa
     1201 aagaagctgt acccagttcc ggaatctgcc ggcctgtcat tatgtgtttt tttaaagacc
     1261 ctatccatct ttgattatga gccttgaatt ttttgtcatc atgaatttcc gttctttttg
     1321 tgcctgctgc cgtaatggct tttaaattct tatatccaaa cactgcaccc acaccacagc
     1381 gccctgctgc acgctcatca cttataactg ctgcataacg gaccatgttt tctcctgcgg
     1441 gtcctattac gaatttccca acacttctgt ttcctaacat atcctgagtc tggccggttg
     1501 tttttcccca taagtgggaa gcgtccttga tttcaatgcg gttttctgaa atttctatat
     1561 atactttttc tttgctcttc ccagatatta caagtccgtc atagcctgca ctttttaact
     1621 taagcccgaa agttccgccg cagtttgaag aagccagaag ttcggttaag ggcgagatgg
     1681 ctgacacgtt aaatctcgat gaggaaggtg agcctgtgcc tgtaaggggg gatgttgtaa
     1741 ttacaattaa attttcttct gaaaaagctt ctgttttttc ctttaatgaa tcatatatta
     1801 tcttagcagc cagggatttt ccgcctaaat acaatattct gtcttcttct ttcaagggat
     1861 aatcagaaat ttctcctgtt gttaaattta ttttagcaat ggtgcctgca tatccgtaca
     1921 tatttatccc tccttaattt tataatatat gcttataata tcttcctttg tcattattct
     1981 tggaacaggg tacaggggat ttgcttcctt aaaagccctc tgcgccatgg ttggtatgtc
     2041 ctcctccttt ataccgctta ttttgtcagg tatgtccatg cttttattca gatttttaat
     2101 cgcatctata aatttgcccg ttttttcttc ctgggagtca ttgatttctc ctatttcaat
     2161 caaatctgca agctccgcca gggctttacc tgcgttgttc ccataaaact caaaaacata
     2221 tggcataatt acagcatttg ccaggccatg aggcatggaa taaaatccgc ccaaggtatg
     2281 tgcaatggca tgcacatagc ccacatatgc cctggtaaag gcaagtccag cataaaatga
     2341 agctttttgc attttagcac gggcttcaat gttgcttccg tttgaatatg cttcataaag
     2401 attatcaaat atcagtttta ttgcttttct tgcataggca tatgtatctt ttgtattact
     2461 tctgccgata taagcttcca ctgcatggca aagagcgtcc atgccggttg atgctgttat
     2521 gtgaggggga agattcaaag tgagtgcagg gtccaatacg gcaacatgcg gtatcaaaga
     2581 catgtcggtt atagagtatt tttcgttagt agtgctgtcc cttactactg cagccacagt
     2641 ggtctcgctt ccggtaccgg ctgttgtggg caccgcaaac aagaaaggaa ttcttttatg
     2701 tacttttaaa agtcctttca tttgcggaat agtcttattt ggtcttgcta ccctggcggc
     2761 aatcccttta gcgcagtcca tgggtgaacc gcctccgaaa gctacaatcc cttcgcattt
     2821 attttgcttg taaaccagca gtgcctcctc aatattgtca atggttggat tgggagtggt
     2881 tttattatag attgcatatt ttatattttt ttcatttaga ccttcaagca aattatttat
     2941 aagacccatt gacgaaatat gactgccagt cacaataagc acattggata tacccttttc
     3001 ttttattgta cccggcagct ttttcaagct gttttcacct tccagcagtt ccggcacccg
     3061 aaagggcata aaattagcgg aaatcttcat aataaattga tagattcggc aaaatacttc
     3121 aaacacaaca attcctcctt tgaatttcac ataattttat tttcattatt aataagaata
     3181 taagtgcaga ctttctttaa aacttgtgta aaggatacaa atttgagagt cttcttgcat
     3241 catcacttta tttaactata acttgtctgc ccttacaatt caaggaattt ttccattgaa
     3301 ttcagggagg agtgtcccca taccatcaat ttcttacagt cagagaaatc gggttggtca
     3361 taaagatcat aagcagctca accatttg
//
LOCUS       DAOPUT010000125         3332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_29204_length_3332_cov_18.805005, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000125 DAOPUT010000000
VERSION     DAOPUT010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29204_length_3332_cov_18.805005"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    55..264
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            493..1743
                     /locus_tag="PKK75_07955"
     CDS             493..1743
                     /locus_tag="PKK75_07955"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNZ83572.1"
                     /translation="MEKEKVVLAYSGGLDTSVIIPWLKENYDNLDIIACCVNVGQEDD
                     MEFVKNKALSSGAIKVYIENAVNEFVEEYVYKGIKANAAYEGKYLLGTAYARPLIAKK
                     LVEVAHKEGAKYIAHGCTGKGNDQVRIETGVAALDPTIKIIAPWRIWDIKSREDAIDY
                     AETKNIDLHGITKEKIYSRDQNILHISHEGGMLENPGNEPDFDEILIMTNTVEKAPDV
                     AEYVEIEFEKGIPVKLNNNKLNGEKMLKALNKIGGTHGIGILDLLENRLVGMKSRGIY
                     ETPGGTILLEAHKYLESFVLEKEEAHFKEMVGNKYAELVYNGMWYSGFKHALDAFIDS
                     TQEKVSGKVKLKLYKGTIKFAGMTSENALYNESISSFSTGELYDQKDAAGFIHLYSLP
                     YKIQAFNEINKNHKSTEVIYTFKE"
     gene            1935..3302
                     /locus_tag="PKK75_07960"
     CDS             1935..3302
                     /locus_tag="PKK75_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ83573.1"
                     /translation="MNGTNGNNQINAMTEGNSFNLILTFAFPMFIGNIFQQLYNMVDS
                     IVVGNYVGKIALAAVGTGFPIIFLMASLFIGLGMGATILISQYIGAGDYENVRKTSQT
                     IYTAMLLSSIPISMLGIIFCEPILLLINTPLDVLPMAKQYMLIIFAGMIGSFGFNINA
                     GILQGFGDSKSSLLFLAIATVINIVLDLLFVMVFGWDVPGVAIATIIAQTFSWIFGII
                     YMKKKYDVLKFKITKFDFDKTIFKKILKLGLPSGIQMSLFSVGVMSMQSLVNGYGADF
                     MAGFNAANKIDTFAFMPIQSISNALTTYTGQNIGGGRLDRVHKGTVTGITVSVTISLL
                     ALIIIPLGPSMLRLFNSEAGVIDAGMIYLNNVMPLYVLLSILFTLNAVVRGAGESIIP
                     MVSSLTSIWLGRIPSAYYIAANLGKEYLFYSYGIGWVLGMLITGTYYVTGRWKTKSQK
                     LLQKQ"
BASE COUNT         1114 a          552 c          720 g          946 t
ORIGIN      
        1 atgttgacaa aatgataaag tcaacatata atcttataaa aatgattaat tgtgtataat
       61 ttaatgattg gaagagtaaa ttaagcaaag cttcagcgag ccgatttaga gtgtgagtcc
      121 ggcagtgtag cttagtcgaa aagaatccat gaaaagattc tcagaagtag cagtatggga
      181 atacggttaa aaaccgttat aatgaaagag cgaagcagct gctttataaa agagtggtac
      241 cgcggtgtga acactgcctc tttatgggca gtttttttat gttctgccac atatgttcga
      301 gctgcagcta agaacagatg gttggcagaa ctaacgcaac gagcaccaaa tttatgcggc
      361 gtaaggagcg aatgcggaga cttcgtatgc tcaatgcgat atgttctctt ttgacacagt
      421 aagaatattc atgcaattat aaatatacat tcaatcattt aatcttaaat attatattcg
      481 gaggaatgga aaatggaaaa ggaaaaagtg gtattggctt attcaggagg attggataca
      541 tcagtaataa tcccctggct taaggaaaat tacgataacc tggatattat agcctgctgt
      601 gtgaatgtag gtcaggaaga tgacatggag tttgtcaaga ataaagcatt atcatccggc
      661 gcaataaagg tttatatcga aaacgcagtg aatgaatttg ttgaagaata tgtatataag
      721 ggtattaagg caaatgcagc atacgaagga aaataccttt tagggacagc atatgcaaga
      781 cccttgattg caaaaaagct tgttgaagtt gcccataagg aaggagcaaa atacattgct
      841 cacggctgta caggaaaagg aaatgaccag gtacgtattg aaacaggagt agcagcattg
      901 gatcccacga ttaaaattat tgcaccatgg cgtatatggg atataaagtc aagggaagat
      961 gcaatagatt atgccgaaac aaaaaatata gatttacacg gaatcacaaa ggaaaaaata
     1021 tattctcgcg accagaatat tcttcatata agccatgagg gcgggatgct tgaaaacccc
     1081 ggaaacgagc cggattttga cgaaattctg ataatgacaa acaccgttga aaaggctccg
     1141 gatgtggctg aatatgttga aattgaattt gaaaaaggaa taccggttaa actgaacaac
     1201 aataagttaa atggggaaaa aatgcttaag gcccttaata aaatcggcgg gactcatgga
     1261 atcggcattc ttgatttatt ggaaaacaga cttgtaggaa tgaaatcaag gggcatatat
     1321 gaaactcccg gcggcactat tcttttggaa gctcacaaat atcttgaaag ctttgtactt
     1381 gaaaaagaag aagcacactt taaggaaatg gtaggtaaca agtatgcaga actggtatac
     1441 aatggtatgt ggtacagcgg ctttaagcat gcacttgatg catttataga ttcaacacaa
     1501 gaaaaggtat cgggaaaagt gaagctcaag ctttataaag gaacaataaa gtttgcagga
     1561 atgacatcag aaaatgcctt gtacaatgaa tcaatttcat cattcagcac cggtgagctg
     1621 tatgaccaaa aggatgcagc cggatttata catttgtaca gcctgcccta caaaattcag
     1681 gcatttaatg aaatcaacaa gaaccacaaa tcaacagaag ttatttacac ttttaaagaa
     1741 tagccatgtt gatagcagga ggcagtctta agagattacc tccttttttt gtgtgaaata
     1801 ggttatggtt atacgccttg ctctttccaa gtatatttca gggattctca acggggcata
     1861 ttcaaaaatt ctcttttgca tttatattat tttatggtat actattaacg cttgacttaa
     1921 ggaggacaaa aatcttgaac ggtactaatg gtaataatca gataaatgca atgactgaag
     1981 gcaattcttt caacctcata cttacctttg ctttccccat gtttatagga aatatttttc
     2041 aacagcttta caatatggtt gacagtattg ttgtgggcaa ctatgtgggg aaaattgcac
     2101 tggcagctgt aggtacaggc tttccgataa tatttttgat ggcatcattg ttcattggct
     2161 tgggaatggg agcgaccata ttgatttctc aatatatagg tgccggagat tatgaaaatg
     2221 taagaaaaac ctcgcaaaca atttataccg caatgctttt aagctctatt ccaatatcaa
     2281 tgctgggcat aattttttgc gagcccatac ttctattaat aaacactcct ttggacgtac
     2341 tgcccatggc aaaacaatat atgctgataa tatttgcagg aatgataggg agttttggct
     2401 ttaatattaa tgcaggcata ctccagggtt tcggtgacag caagtcatcg cttctgtttc
     2461 ttgctatcgc aacagtaatc aatatagtgc ttgacctctt gtttgtaatg gtatttggct
     2521 gggatgttcc gggagttgca attgcaacaa taatagcaca aactttttcc tggatttttg
     2581 gaattattta catgaaaaag aaatatgacg ttttgaaatt caaaataacc aagtttgatt
     2641 ttgacaaaac aatttttaag aaaatactta agctgggtct gccttcggga atacaaatgt
     2701 ccttgttctc agtaggagtt atgagcatgc agtcattggt aaacggctac ggggctgact
     2761 tcatggcggg cttcaatgcg gcaaataaaa tagatacatt tgcatttatg cccatacaaa
     2821 gcatttcaaa tgcgcttaca acatacaccg gacagaatat aggcggcgga aggctggaca
     2881 gggtgcataa gggaacagtt accggaatta cagtgtcagt cacaatcagc ctcctggctc
     2941 taataattat acccctgggg ccgtctatgc ttagattatt caattcagaa gccggcgtaa
     3001 tagatgcggg gatgatatac ttaaataatg taatgccttt gtatgttctg ctttcaattt
     3061 tatttacctt gaatgccgtg gtaagaggag caggggaatc aataattccc atggtcagct
     3121 ccctcacatc catttggctt ggcaggattc cttcagctta ctatatagca gcaaatctgg
     3181 gtaaggaata cttgttttac agctacggca taggctgggt tttgggaatg ctgattaccg
     3241 gaacctatta tgtaacaggc aggtggaaaa caaaatcaca aaaattattg caaaaacaat
     3301 aaaaaaggga cacaatgcgt gtccattttt tt
//
LOCUS       DAOPUT010000126         3287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_29749_length_3287_cov_19.479889, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000126 DAOPUT010000000
VERSION     DAOPUT010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29749_length_3287_cov_19.479889"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PKK75_07965"
     CDS             complement(<1..1376)
                     /locus_tag="PKK75_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNZ83574.1"
                     /translation="MIDINKILCEEFNLKPFQVDNTVKLIDEGNTIPFIARYRKEQTG
                     SLDDVVLRDLFERLTYLRNLELRKEEVLRLIEEQGKLTEDLKNEILSADVLQRVEDLY
                     RPYKQKKSTRATKAKEKGLEPLAEIILAQNVLEGDLDEIAGPFINREKGVNSVKEAYE
                     GACDIIAEIVSDNADYRKHIREVYVNDAVLMSEAADEEEKTVYEMYYKFEEPVNKIAN
                     HRILAINRGEKEKKLKVKLSAPDEKIINYLVSKEIKNESAITTDYYLAAIQDSYKRLI
                     APSIEREIRTMLTERAEEEAIKVFGKNTKNLLMVPPVKDVRILAIDPSFRTGCKLAVL
                     DGTGKFLEEGVMYPNEPRNEVEKSQKQMAELIKKFDIDVITIGNGTASRETEMVVSDM
                     LSKYTFPKKVTYTIVSEAGASVYSASKLAQEEYPELDVTIRGAISIGRRLQDPLAELV
                     KIDPKSIGV"
     gene            complement(1626..2760)
                     /gene="prfB"
                     /locus_tag="PKK75_07970"
     CDS             complement(join(1626..2660,2662..2760))
                     /gene="prfB"
                     /locus_tag="PKK75_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157593255.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNZ83575.1"
                     /translation="MIYKGDDKLTDWYDITERIKKFNDILNEVGGSLDPEKLREENFT
                     LESQMQEPGFWKDQEKAQKASQKLKHNTNRMNEFNSLKQQVEDIELIVELIQEEEDES
                     HVPELLKSMDEAEEQLEDARIVALLSDEYDENNAILSIHAGAGGTEAQDWADMLLRMY
                     TRWIESKGYRMDIYDIQRDTEAGIKSVTIFVEGENAYGYLKSEKGVHRLVRISPFDSS
                     NKRHTSFASVDITPEIDDDEDIELNEADLKIDTYRSSGAGGQYVNTTDSAIRITHIPT
                     GVVVSCQNQRSQHSNKETAMTMLKSKLMEIRKIENKEKIEDIQGKYNQIAWGSQIRSY
                     VFHPYSMVKDHRTDAETSNVQSVMDGNLDMFMNEYLKMKSKER"
BASE COUNT          800 a          732 c          589 g         1166 t
ORIGIN      
        1 acaccgatgc ttttgggatc aatcttaaca agctcggcca aggggtcctg aagtcttctt
       61 cctattgaaa tagctcctct tattgttaca tcaagctcag ggtactcttc ctgggctaat
      121 ttggaagctg aataaactga agctcctgcc tcgcttacga ttgtataggt gacctttttc
      181 gggaatgtat attttgacag catgtccgat acaaccattt ctgtttctct tgaagctgtt
      241 ccgtttccta tggtaataac atctatatca aattttttga taagttcagc catctgtttc
      301 tgtgactttt caacttcatt tcttggttcg ttgggataca ttaccccttc ttccaaaaac
      361 ttgcctgttc cgtccaggac ggctaatttg catcctgttc gaaagcttgg gtctatggca
      421 agaatcctta catccttaac gggaggaacc atcagcaggt tcttggtatt ttttccgaat
      481 accttgattg cctcttcttc agctctctcc gtaagcatgg tcctgatttc cctttcaatg
      541 gaaggagcaa taagcctttt gtagctgtcc tgaattgccg ccaggtaata atctgtagtt
      601 atcgcacttt cattttttat ttcttttgat accaggtagt tgattatttt ctcgtctgga
      661 gcactcagct taacctttaa tttcttttcc ttctctcctc tgttaatggc aagaatcctg
      721 tgatttgcaa ttttattaac cggttcttcg aatttataat acatttcata gacggtcttt
      781 tcttcttcat cagcagcttc cgacatcagt acagcatcat ttacataaac ttcacgtata
      841 tgctttctgt agtctgcatt gtctgaaaca atctcggcta ttatgtcgca ggcgccttcg
      901 tatgcttcct ttacactgtt gacgcccttt tccctgttta taaagggacc tgctatttca
      961 tcaaggtctc cttcaagtac attttgtgcc agaatgattt cagccagggg ctcaaggccc
     1021 ttttccttcg cctttgtcgc ccttgtagat tttttctgtt tgtagggtcg gtataagtcc
     1081 tccactcttt ggagtacatc tgcgctaagt atttcgtttt taaggtcttc tgtaagcttt
     1141 ccctgctctt cgattaaacg gagcacctct tcttttctca gctccaggtt tctcaagtag
     1201 gtgagtcttt caaacaggtc tctcaagacc acatcatcaa gggatcctgt ctgttcctta
     1261 cggtatcttg caatgaaggg aattgtattg ccttcatcta tgagctttac cgtgttatca
     1321 acctgaaagg gttttaaatt aaattcttca cacaatattt tattaatatc tatcattttt
     1381 ctctccttct cgcagtctca ttcaccaatt tatatattcg gggacattcc tcatgcccac
     1441 aaagactttc tcaatagtag aggtgtgtcc ccatacatat tgtgtcgcat aaattgggaa
     1501 ttcgttgcaa atgacctacc gcaatttttc agaaaatcat attcggggac attcctctta
     1561 ccctaaagac tatctctaaa gtagaggtgt gtccccatac ctctaataaa tagggttagg
     1621 gcatcttatc tttcctttga tttcattttt aaatattcat tcataaacat atcaagattg
     1681 ccgtccatta cagactgaac atttgatgtt tccgcatctg tccggtggtc cttcaccata
     1741 ctgtaggggt ggaagacata ggaccggatt tggcttcccc aggcaatttg attgtatttt
     1801 ccctgaatat cctctatctt ctccttattt tcaatctttc ttatttccat aagctttgac
     1861 tttaacatgg tcatggctgt ttctttgttg ctgtgctgag acctttggtt ttggcaggat
     1921 actactacgc cggttggaat atgggttatt cttattgcag agtcggtagt atttacgtac
     1981 tgtcctcctg ccccgcttga ccggtaagta tctattttta agtctgcttc attcagctca
     2041 atatcctcat catcatcgat ttcaggtgtt atatccactg atgcaaagga tgtgtgcctt
     2101 ttgtttgaag agtcaaaggg tgatattctg acaagcctgt gcacgccctt ttcagatttc
     2161 aaatatccgt aagcattttc tccctcaaca aaaatggtga cgcttttaat ccctgcttca
     2221 gtatctctct gaatatcata tatatccatc ctgtagccct tggattctat ccacctggtg
     2281 tacattctaa gaagcatgtc tgcccagtcc tgtgcctctg ttcctccggc tcccgcatgt
     2341 atggacaaaa ttgcattgtt ttcatcatat tcatcactta aaagagcaac aattctggca
     2401 tcttccagtt gttcttctgc ttcatccatg ctttttagaa gttcaggcac gtggctttcg
     2461 tcttcttctt cctgtatcaa ttcaacaata agctcaatat cctccacctg ttgtttcaaa
     2521 ctgttgaatt cattcattct gtttgtgtta tgcttaagct tttgagatgc cttttgtgcc
     2581 ttctcctggt ccttccaaaa tcctggctcc tgcatctgtg attccaaagt aaagttttct
     2641 tccctcagct tttccgggtc aaagggaacc accaacctca tttaatatat cgttgaactt
     2701 tttgattctt tctgttatat cataccagtc cgtcaattta tcatctcctt tataaatcat
     2761 tttcttaata tacacaactt attgaaaaat aataaaaata ttaagcgaac atttgcagga
     2821 cacgactata attcttagcg aaggtcggtt aaaaatccgt taagcagctt gcactgtttg
     2881 agcgcagcga gtttgcaagc tgtaggattt ttagcgggaa tgagctttag aattattcgt
     2941 ggccgaaacc gtgagcgaat atttacatca gcttttgaaa aataataaaa atattaagcg
     3001 aacatttgca ggacacgact ataattctta gcgaaggtcg gttaaaaatc cgttaagcag
     3061 cttgcactgt ttgagcgtag cgagtttgca agctgtagga tttttagcgg tcatgagctt
     3121 tagaattatt cgtggccgaa accgtgagcg gatatttaca tcagcttttc aaaaataata
     3181 aaaatattaa gcgaacattt gcagaacacg actataattc ttagcgaagg tcggttaaaa
     3241 atccgttaag cagcttgcac tgtttgagcg tagcgagttt gcaagct
//
LOCUS       DAOPUT010000127         3230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_30495_length_3230_cov_20.106142, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000127 DAOPUT010000000
VERSION     DAOPUT010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30495_length_3230_cov_20.106142"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1550
                     /locus_tag="PKK75_07975"
     CDS             183..1550
                     /locus_tag="PKK75_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ83576.1"
                     /translation="MRTIFKDKTFLKAMIAIALPITLQNLVASSVNMLDTLMITSLGE
                     ESLAAVGLANQVFFFYAVTIFGVATGSSVFVSQFWGKGDVKNIGRILGISLAITSVLG
                     ALFTLAALFVPEFIMKIFSEDSEVIRLGVDYLRIVSLSYIITGISFAYAVASRSIGQA
                     KMPMIASIVSFLVNLIFNYLLIFGKFGFPQLGVKGAAYGTIIARAAEIGLMLYSIYSN
                     KNNNPLAHSFKSMTDWTGDFIRKYFKTAYPVILNEALWSLGTVLYSLAYAKIGTEAAA
                     AVQILNTVQNIFMVLSRGLANACTVMVGNKIGADEEDEAVVYADNFMILSAALGLVLG
                     VLIYFSKDLILIFFRNLTPSMYNTSRNLLIVLAASFFIRSFNGTLIVGVLRGGGDTRF
                     SMLLEMGSVWLVGVPLAFLGALVFRFPVYYVFLLVNIEEIVKAAIGILRVKSKKWVTN
                     IIHDM"
     gene            1633..3225
                     /locus_tag="PKK75_07980"
     CDS             1633..3225
                     /locus_tag="PKK75_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNZ83577.1"
                     /translation="MNITELINKRRIFGIISHPDAGKTTLTEKLLLHGGAIRQAGTVR
                     ARKNARYAKSDWMEIEKQRGISVTSSVMQFEYDDKVISIMDTPGHNDFGEDTYRILTS
                     VDSAVMVIDAAKGIEVQTKKLFQVCSMRGIPIFTFINKLDREAMDPLESMQELEDVLG
                     LPSVAVTWPIGCGKDFEGIYDRQRNVVNLFKKNELVNLGDEGVKSSKLDEKINPFNLA
                     SLRNAMELLDGAGNVFDMELVRQGKLSPVFFGSALADFGVTEFLKHFLIMSPPPSSRK
                     TTTGEVQPSDDTFTGFIFKIQANMDPNHRDRLAFLRICSGTFIRGMNVTLSRTGRQMK
                     LSQSTHLMANERETVDTAYAGDVIGIYDSGNFQIGDTLTNGRETIFFEPLPTFPPELF
                     SRVSPKNSLKGKNFQKGVEQLAQEGAIQVYRNEYNEIILGAVGVLQFEVFEYRLANEY
                     NTEIRMENIEFTVARWVKTDNPESLKKYQNSRCMLVFDHYERPVLLFTNQYALRSFQD
                     RYENVELVEALDVVDELENTVI"
BASE COUNT         1047 a          537 c          686 g          960 t
ORIGIN      
        1 catcttgagt attataacat tttacccgct tttgttcaat aaaaaaattc ccctgtcctg
       61 ctcatccccc atttgtcatt aaaatgaaaa atactagttc aatttatgaa acatttgtgc
      121 tattatatat aagttgaaaa tgacataata aaaagcaact gtattgttgt tcaggaggac
      181 aaatgaggac tatttttaaa gacaaaacat ttctaaaagc tatgatagcc atagccttac
      241 ctataacctt gcagaactta gtggcttctt ctgtgaatat gcttgataca ttgatgataa
      301 caagcctggg agaggaaagc ctggctgctg ttggactggc aaatcaggtc ttcttttttt
      361 atgccgttac catatttggt gtagccacgg gttcatcagt atttgtgtca caattttggg
      421 gtaagggaga tgttaaaaat atagggagaa ttttaggaat atcattggct ataacaagtg
      481 ttttaggagc tttatttact ttagcagcat tgtttgtgcc tgaatttata atgaaaattt
      541 tcagtgaaga cagtgaagtt ataagactgg gggttgatta tttaagaatt gtttccctta
      601 gctatattat tacgggaata agttttgctt atgctgttgc atcaaggagt atcggacagg
      661 ctaaaatgcc catgatagca agcatagttt cctttctcgt caatctgata ttcaattatc
      721 ttctcatctt cggaaaattt ggtttcccgc agctgggagt caaaggagca gcctacggta
      781 ctataatcgc aagggctgct gaaataggcc tgatgctgta ttcaatatat tcaaataaga
      841 ataataaccc tcttgcccac agcttcaaaa gcatgacaga ctggacagga gattttatta
      901 gaaaatactt taaaacagct tatcctgtta ttttgaatga agcactttgg tctttaggta
      961 ctgtgcttta ttctttagct tatgcaaaaa tcggaactga agctgctgct gcagttcaaa
     1021 ttttaaacac tgtacaaaac atatttatgg tactcagcag ggggctggca aatgcatgca
     1081 ctgtcatggt aggcaacaaa ataggtgctg atgaagaaga cgaagctgtt gtatatgctg
     1141 ataatttcat gatattatcc gcagcattag gccttgtatt aggagtactc atatattttt
     1201 caaaggatct gattcttata ttctttagaa atctcacacc aagcatgtat aatacttcaa
     1261 gaaacctctt aatagtgctg gcagcttcat tctttataag gtcatttaac ggtaccttga
     1321 ttgtgggagt attaagaggc ggcggggaca caaggttttc gatgctgctt gaaatgggct
     1381 ctgtctggtt agttggagtt cctttggcat ttttaggtgc attggtattt aggtttcctg
     1441 tatactatgt tttcttgttg gttaacattg aggaaattgt aaaagctgca attggaatat
     1501 taagagtaaa atcaaaaaaa tgggttacaa atataataca tgatatgtag ataataaaat
     1561 aatcctttat aattttgtcg aaatattgta gaatataaaa gattattatt ttaccggata
     1621 ggatggaaag aaatgaatat tacagaatta ataaataaaa gaagaatatt tggtattatt
     1681 tcacaccctg atgcaggaaa aacgaccttg accgaaaaac ttctgctcca tggaggagcc
     1741 ataagacagg caggtacagt acgtgcgagg aaaaatgcca ggtatgccaa atcagactgg
     1801 atggaaatag aaaaacagag aggtatctct gtaacttctt ccgttatgca gtttgaatat
     1861 gatgataagg taatttcaat aatggataca ccgggacaca atgacttcgg tgaagacact
     1921 taccgtattt taacatctgt agacagtgcg gtaatggtta ttgatgccgc caaaggtata
     1981 gaagtacaga caaagaagct gtttcaggtt tgcagcatga gaggaattcc catttttaca
     2041 tttataaata aattagatcg cgaagcaatg gaccccctgg aatccatgca ggaactggaa
     2101 gacgtgctgg gtcttccttc cgtagctgtt acgtggccta ttggatgcgg caaagatttt
     2161 gaaggaattt acgacagaca aagaaatgtg gttaacctat tcaagaaaaa tgagctcgta
     2221 aatcttggtg atgaaggcgt caaatcaagc aaactggatg aaaaaataaa tcctttcaat
     2281 ctggcaagct taagaaatgc catggagctc cttgacggtg caggaaatgt atttgatatg
     2341 gaacttgtaa gacaaggcaa gctgtcaccg gtattttttg gttctgcatt ggctgatttt
     2401 ggtgttacag aatttttaaa acattttctt attatgtctc ctcccccgtc ttcaagaaaa
     2461 accacaacgg gagaagtcca accatctgat gatacattta caggctttat atttaaaatt
     2521 caagcaaata tggatccaaa ccaccgtgac aggctggcat ttttaaggat ttgctctggg
     2581 acattcataa gaggaatgaa tgtaactctt tcaaggaccg gaagacagat gaagttaagc
     2641 cagtccaccc acctaatggc caatgagcgt gaaacagtag atacagcata tgcaggcgat
     2701 gtaataggaa tttatgattc gggaaatttc caaatcggcg atacacttac aaacggaaga
     2761 gaaacaatat tttttgagcc cctgccaacc ttcccgccgg aattattcag cagggtaagc
     2821 cctaagaatt ctctcaaggg aaagaacttt caaaaaggtg tagagcagct ggcacaggaa
     2881 ggtgcaatcc aggtttacag gaatgaatac aacgaaatta tcttgggggc tgttggagtt
     2941 ctgcagtttg aggttttcga ataccggctg gctaacgaat acaatacgga aattcgaatg
     3001 gaaaacatag aatttactgt agcaagatgg gttaaaacag ataaccccga aagcttgaaa
     3061 aaatatcaaa attcacgctg catgctggtt ttcgaccact acgaaagacc tgtgctcttg
     3121 tttaccaacc agtacgcact aagaagcttc caggacagat atgaaaatgt ggaattggta
     3181 gaagcactgg atgtagtgga tgaattagaa aatacagtaa tataagtttt
//
LOCUS       DAOPUT010000128         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_30711_length_3213_cov_17.808423, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000128 DAOPUT010000000
VERSION     DAOPUT010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30711_length_3213_cov_17.808423"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      65..178
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            283..894
                     /locus_tag="PKK75_07985"
     CDS             283..894
                     /locus_tag="PKK75_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNZ83578.1"
                     /translation="MKSDVTLKQRSSAKIVAKVGVLGAVATLLMLLDFPLWFAPSFYK
                     LDFSEVPVLIGAFALGPLAGAMIELVKILLNFVLNGTDTGGIGEFGNLLVGLSFIVPA
                     GFIYKKFKTFKSAITGMLVGTISLAVMGAFLNYFLLLPVYSKIYGAPIQAFIDMAKVL
                     NPAITDLKTFIMYAVVPFNIFKGMVTSAMVILIYKKLSPILHR"
     gene            1013..2248
                     /locus_tag="PKK75_07990"
     CDS             1013..2248
                     /locus_tag="PKK75_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ83579.1"
                     /translation="MKLQIYSLIMFLNSYLTGLLTPVLSLALIEKGASLSNLSIILGI
                     YALSVILLELPSGIMADVFGRKKTYLLSLVSFTLSFSLLLTSRGFIVLCVVMTLYGFG
                     RALGSGSFDALFIDYYIDNYGKDKLHNITTRLSVLEALGLSAGALSGGFFPEISLELF
                     PAIGIYDLNIIIRIILTALVIVLSVLFISENVRHDNKELVSIKQHIKNSSNFVFKNVT
                     ILCIFLSVFSTGFFLSALETYWQPHFITFLPDESSTRLLGLMAFLYLGAAVLGSILSN
                     KTIKKSKFNLRKMYLLMRAAIAFLLILTALQTNIPVFIAFYASIYLVFGMANIPEGVI
                     LNHETPNEIRASVLSVKSFTLQIGGLSGSLLYSILIRFMSIPVIWMVAAFIVLFSTAL
                     TARKLIKAEEVMFEYTSQA"
     gene            complement(2778..3117)
                     /gene="rnpB"
                     /locus_tag="PKK75_07995"
     ncRNA           complement(2778..3117)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKK75_07995"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          926 a          604 c          598 g         1085 t
ORIGIN      
        1 tattatcaaa tatttaataa aaaggtgttt acaaattcca ggttcgtgat ataatagaaa
       61 atgtaaatat cttcagggca gggttaaatt cccaaccggc ggtaaagtcc gcgaaccgaa
      121 aggctgaatt ggtgaaattc caataccgac agtagagtct ggatggtaga agagattact
      181 tgcaaaattg tgagcaaatt ctttcgacgc ctctgaagat ttcagaggtt tttatatgcc
      241 tctgataatc ttagaatagt gcttagcaca aggaggaata taatgaaaag tgatgtaaca
      301 ttgaaacaaa gaagcagtgc aaagatagta gcgaaggtgg gagtattggg agcagtagca
      361 actttattaa tgttactcga tttcccgcta tggtttgcac caagttttta taagctggac
      421 ttcagcgagg taccggtatt aataggtgca tttgccttag ggcctttagc cggagctatg
      481 attgaactgg taaagattct tttaaacttt gtgctcaacg gaactgatac aggtggaatc
      541 ggagaatttg gaaacctttt ggtaggattg tcattcattg ttccggcagg attcatttac
      601 aaaaaattta aaacatttaa atcagctata acgggaatgc tggttggaac aatatcctta
      661 gcagttatgg gagcattttt gaattatttc ttattgcttc ctgtatattc taaaatttat
      721 ggtgcaccaa tccaagcatt tatagatatg gcaaaagtat taaatccggc aataacagat
      781 ttaaaaacat ttattatgta tgcagttgtt cctttcaata tatttaaagg tatggtgaca
      841 tcagctatgg taatattaat ttataagaag ctttcaccga tacttcacag atagttttca
      901 ttcttataaa ttgtcttaga cgcatgaaaa tgcgtctttt ttattccttg actgaaatac
      961 tcttttcgat tataattatt ctaccgttgc attatttacc tctggaggta atatgaagct
     1021 tcaaatatac tcattaataa tgtttctgaa ttcatatttg accggattat taaccccggt
     1081 actcagcctg gctttaattg aaaaaggagc ttcattaagc aacttgtcaa ttatattagg
     1141 tatttatgcc ctgtcggtta tcctgcttga acttccttcc ggtataatgg cagatgtttt
     1201 cggccgtaaa aagacatatc ttttatcgct tgtttctttc accttaagct tttccctgtt
     1261 attaacaagc aggggtttta tagttttatg tgttgtaatg acgctttacg gttttgggag
     1321 agctcttggt tccggatcat ttgatgcatt gtttatagat tattacattg ataattacgg
     1381 caaagacaaa ttgcataata taacaacaag actatccgtt ttagaagcat tggggctgtc
     1441 agccggtgca ttgtccggag gattcttccc tgagatatcc cttgaacttt ttcctgccat
     1501 aggaatttat gatttaaata taattattag aataatttta acagccctcg taatagtatt
     1561 atctgttctt tttatttccg aaaatgtccg acacgacaat aaggaacttg tttcaatcaa
     1621 gcagcatatc aaaaatagtt ctaattttgt ctttaaaaat gtaactatac tatgtatttt
     1681 tctttctgtt ttttcaacgg gtttttttct tagcgcctta gaaacctact ggcagcctca
     1741 ctttataaca ttcctgcccg atgagagctc taccaggctc ttgggactta tggccttttt
     1801 gtatttggga gcagcagttt taggaagtat cctttcaaat aaaactataa aaaaatctaa
     1861 attcaatcta agaaaaatgt acctgctcat gagggcagct attgcatttt tattgatact
     1921 tacggcactt cagacaaata ttcctgtgtt tatagccttt tatgcttcaa tttatctggt
     1981 gttcggcatg gctaacatac ctgaaggtgt aatacttaac cacgaaacgc ccaatgaaat
     2041 aagggcttct gttctgtctg taaaatcatt tacattgcag attggcggtc tttccggttc
     2101 attattgtac agcattttaa tcaggtttat gtccatacct gttatatgga tggttgcggc
     2161 tttcatagta ttgttttcaa ctgcattaac tgcaagaaaa ttaattaaag ctgaagaagt
     2221 aatgtttgaa tatacatctc aggcataaga cctggcaagt tttaaccaca tgcttcatgc
     2281 cgtattacaa aaaattaaac ctgaaacatt tgttgtttca ggttttttgt aaggaacacg
     2341 catatccctc ttcttagcac ctacgtctgc agcatgagtt gcatcttccc cttgctccta
     2401 agacatcccg tctttcgctg catttaccaa aaccaacgcc tagtaaaaac catggcaata
     2461 ttctgcaaaa gcaaaacata atcatcatcc ctttttattc agcctgtact tattttattc
     2521 aagctttaca gttttgttac aaaaaaggtg acctgggccc ataaatcaga aaaatatgcg
     2581 gctataggga gctgataatt tggggaatga aactgagctt ttatcatggc agattaaatt
     2641 aaaataggga catgcctcta tccttgaagg ctatcccaaa gaggatattc tttcaagatg
     2701 tgtccccata caaattcggg gacattcctc tgacccgcga aggctatccc tgcggaagag
     2761 gtgtgtcccc ataccttatt gtgagtaaat atataagccg agttctgtat taaacaatca
     2821 tctatctagg tctgctgttg ccggcagact caagcgacct accatttgga ccgtgacgag
     2881 cagccactcc agggtccttt tttggtcttg ctccgggcgg ggtttacata gccagtcagt
     2941 cgcctgactg ctggtgagcc cttacctcac ctttccatcc ttgccgcgca ataatgcggg
     3001 cggtttcttt ctgttgcact atccttgaag tcgccctcac cgggtattac ccggcgccct
     3061 gctctatgga gctcggactt tcctcatgct taagcatgcg attgtataat ttactcttta
     3121 gtattttata atatttttat tttaatgtca aatgtagaat tatccaatat tcatttcctg
     3181 atattttcac attttttaat ccataaatca aag
//
LOCUS       DAOPUT010000129         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_30779_length_3209_cov_18.822765, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000129 DAOPUT010000000
VERSION     DAOPUT010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30779_length_3209_cov_18.822765"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PKK75_08000"
     CDS             <1..317
                     /locus_tag="PKK75_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HNZ83580.1"
                     /translation="NSLNIKKASREYLPMEIGGHARECIGEAMLSFKEGFDGAIQIFP
                     MGCMPEIVSKSILPSISKDYDFPIMSLVVDDMTGEAGYITRVEAFLDLLERRRDNVLY
                     GS"
     gene            298..1269
                     /locus_tag="PKK75_08005"
     CDS             298..1269
                     /locus_tag="PKK75_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNZ83581.1"
                     /translation="MYYMGVDVGSVSTDIALIDKDYNLVEKIYLRTKGNPIKAVQEGF
                     KILKNKYEDREISAVGTTGSGRMIASAIIGADTAKNEITAHAAAALEIDKDVRTIIEI
                     GGQDSKIIIINNGIVSDFAMNTVCAAGTGSFLDRQAERLDIPIEDFGDYAMTASSTVR
                     IAGRCAVFAESDMIHKQQLGYNNAEIIRGLCDALVRNYLNNIGKGKTILPKVLFQGGV
                     AANKGMKKAFENELGFEIFVPENFSMMGAIGAAIIASEKVNKTGKTNFRGLKLADNNI
                     FSRSFECEGCPNKCEVVKIYENINIIGYFGDRCTKWSNKTTQKSKLA"
     gene            1356..2996
                     /locus_tag="PKK75_08010"
     CDS             1356..2996
                     /locus_tag="PKK75_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ83582.1"
                     /translation="MLKEDDFPFLSVQNYESTLKKIESTWENFIKGETIPDFNLPRNP
                     IFESWKRSKQMKVPFDLKKAPLRLNKSELAAEKESKYDLYRIIDSFLDNIFKIFHDEN
                     MAVSFSNERGIVMSVRAGKAMQEKCGLISFMPGSSLRESDFGTNSIGTSLYTKKPIQV
                     YATEHYCETAHTLNCSSALIREPSSGIVLGVLTLTTHKNIVPAHTLNWTISEAEKIQN
                     AIKYHFQKENAFANTINNYKATGDCGEFKCIIGKNPAFNNVIDLAYKFSDSDMPVLIT
                     GDTGTGKEVLARAIHNRSSRKHKTFLPVNCGAIPKELISSELYGYVEGAFTGTQKGGR
                     IGKIEASSGGTLFLDEICEMPVDMQIYLLRALEEGTITRVGSNKAIPVNLRIICATNK
                     DIREEVKKGNFRKDLYYRLNGVELHLPTLRERGDDIILLAEHFMKLKSNELKLSEDAV
                     KVLMEYSWPGNVRELKSVINRAVLISSNFIITSNEIHITDNKQELEKSKQEINEKQLT
                     SEKIKQVINECKGNKTLAANHLDISRMTLYRKIKELNL"
BASE COUNT         1189 a          526 c          666 g          828 t
ORIGIN      
        1 tcaattctct taatataaaa aaagcatcgc gtgaatatct gcccatggaa ataggaggtc
       61 acgcaagaga atgcatagga gaagcaatgc tttcatttaa agaaggattt gacggagcaa
      121 ttcaaatttt ccccatggga tgcatgcctg aaatagtatc caagtcaata ctgccttcta
      181 tttcaaaaga ttatgacttc cccataatgt cgcttgttgt ggatgatatg acaggagaag
      241 cagggtatat aacaagagtt gaagcatttt tagatttact tgaaaggaga cgggataatg
      301 tattatatgg gagttgacgt aggctcagta agcacggata ttgctcttat tgataaagat
      361 tacaaccttg ttgaaaaaat atatttgaga acaaagggaa accctatcaa agcagttcag
      421 gaagggttta aaatattaaa aaacaaatat gaggacaggg aaatttccgc tgtgggaact
      481 accggcagcg gaagaatgat cgcttcagca ataataggtg cagataccgc caaaaatgaa
      541 ataaccgccc atgcagcggc agcccttgaa attgacaagg atgtaagaac cattattgaa
      601 ataggcgggc aggattcaaa aataattata ataaataacg gaatagtcag tgactttgcc
      661 atgaatactg tttgtgcggc aggcaccggt tcatttttag accggcaggc agaaaggctt
      721 gacataccaa ttgaagactt tggagattat gccatgaccg cgagctcaac cgtacgtatt
      781 gccggcaggt gcgctgtatt tgctgaatcg gacatgatac acaagcagca gctcggctat
      841 aataatgcgg aaataataag aggactttgc gatgcacttg tgaggaacta tttaaataat
      901 ataggcaaag gcaaaacaat tctcccgaag gttttattcc aaggaggagt tgcagcaaac
      961 aagggcatga aaaaggcctt tgaaaacgag ctgggatttg aaatatttgt tccggaaaat
     1021 ttcagtatga tgggagccat aggtgctgca ataatagcaa gtgaaaaagt aaataagacg
     1081 ggcaaaacaa atttcagagg attgaagctt gctgataaca acatattttc aagaagcttt
     1141 gaatgcgaag ggtgccccaa taaatgcgaa gtggtaaaaa tatatgaaaa tattaatatc
     1201 ataggatatt ttggcgacag gtgcacaaaa tggagcaata aaactacaca aaaatccaaa
     1261 ttggcatgaa aacgtccccg ttgacaaaat cacttaaaaa tcgtataata aataatgttt
     1321 cataataaaa attaagtgat gcaggtgggc tataaatgct taaagaggat gattttcctt
     1381 ttttgtcagt gcaaaattat gaatcaacat taaaaaaaat agaatcaaca tgggagaatt
     1441 tcattaaagg tgaaactatc cctgatttta atttgcccag gaatccaata tttgaatcgt
     1501 ggaaaagatc aaaacagatg aaagtaccat ttgacctgaa aaaagctcct ctcagattga
     1561 ataaatctga gcttgcagct gaaaaagaaa gtaaatatga tttatacagg attatagact
     1621 cattcctgga taatatattt aaaatattcc acgatgaaaa tatggcggtt tccttcagca
     1681 atgaaagagg tattgtcatg tctgtcagag ccggaaaagc tatgcaggag aagtgcggct
     1741 taattagttt tatgccgggg tcgagcttga gagaaagcga ttttggcacc aacagtatag
     1801 gtacatcatt atacacgaaa aagcccatac aagtttatgc aaccgaacac tactgcgaaa
     1861 cagcacatac attaaactgt tcatctgcac tcataagaga accatcttca gggattgtat
     1921 tgggagttct gactcttaca acacacaaaa atattgttcc tgcacatact ctgaactgga
     1981 ccatcagcga agcagagaaa atccaaaatg caattaaata tcatttccaa aaagaaaacg
     2041 catttgccaa tacaataaat aactataaag caacgggcga ttgcggagaa ttcaaatgca
     2101 taatcggcaa aaatccggca tttaacaatg tgattgattt agcttataaa ttttccgact
     2161 cggacatgcc tgtattgata accggcgata caggaacggg taaagaagta ttggcccgtg
     2221 ctattcataa tagaagcagc cggaagcata aaacctttct gccggtaaac tgcggggcaa
     2281 tacccaagga gttaatttca tcggaattat acggatacgt tgagggagct tttacaggaa
     2341 ctcaaaaggg cggcaggatt ggtaaaattg aagcatcaag cggaggaact ctgtttcttg
     2401 atgaaatatg cgaaatgcca gttgatatgc agatttatct tttgagagca ttagaggaag
     2461 gtacaattac cagggtaggt tcaaataagg ccataccggt aaatcttcga atcatatgcg
     2521 ctaccaataa ggatataaga gaagaagtaa aaaaaggcaa ttttcgtaaa gacctttact
     2581 acagactgaa tggagtagag cttcatcttc caacactgag ggaaagagga gatgatatta
     2641 tactgcttgc cgaacatttt atgaagctga aatcaaatga attaaagctg tcagaagatg
     2701 ctgttaaagt attgatggaa tattcatggc ccggcaatgt gcgcgaatta aaaagcgtta
     2761 taaacagggc agtcttgatt agcagcaact tcattattac atccaacgaa attcacatta
     2821 ctgacaataa acaagaactt gaaaaaagta aacaggaaat aaatgaaaaa caacttacat
     2881 cagaaaaaat aaaacaagtc atcaatgaat gtaaggggaa taaaaccctc gcagcaaacc
     2941 acttggatat ttcacgcatg accttgtata gaaaaattaa agaattgaac ttataaatta
     3001 agctgccgct ttgatatgat tcatccaaag ggcagttttt ttatggagat attcagtgca
     3061 attctaatgg ctttatagcg aaaatttcat aaatgttaca catgttcctg ttacagatgt
     3121 aacaaagtga caaaaaagtt gacacttatt cgataaaaat ctgcaagaaa ttaataaaat
     3181 tatttggcat gaaaattgct atataatat
//
LOCUS       DAOPUT010000130         3200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_30904_length_3200_cov_21.470270, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000130 DAOPUT010000000
VERSION     DAOPUT010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30904_length_3200_cov_21.470270"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKK75_08015"
     CDS             <1..531
                     /locus_tag="PKK75_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNZ83583.1"
                     /translation="LSLPMFIKPANNGSSIGISKVRNYDEFEKGIMEAFEYDRKIVLE
                     SNIEGFEIGCAVVGNDELLIGEVDEIDTKNDFFDYVEKYSQHNSKIYCPARISDDLKA
                     RAKEIAAKTYKALGCSGLSRVDMFLTPENDIYLNEINTIPGLTDLSRYPSMLKKIGIE
                     YKDLIVKLVDLAMERR"
     gene            complement(598..891)
                     /locus_tag="PKK75_08020"
     CDS             complement(598..891)
                     /locus_tag="PKK75_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83584.1"
                     /translation="MDQESMVFEGIIRKLNIDSSDDKEIENNTNTGYKALISCITNLP
                     PKQFVLLSTLIGIFLIDGIDAKDQIILGKFINNIGQTIFTAAAQEKINIKNGN"
     gene            complement(1074..1892)
                     /locus_tag="PKK75_08025"
     CDS             complement(1074..1892)
                     /locus_tag="PKK75_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNZ83585.1"
                     /translation="MEKLIITAAICGAEVTKEHNPAVPYTIEEIVREAKSAYDAGASI
                     IHLHVRWDDGTPTQDTKRFQEAVDAIKAVCPDVIVQPSTGGAVGMTDLERLASTDIVP
                     TPEFATLDCGTCNFGGDEIFINTDNTIFNFAKIMKEKGIKPELEVFDKGMIDIALKAD
                     RKGLLVHPLHFDFVLGVQMSATIRDLVFMVGSLPQGSTWTATGIGKSTWTIAAATIAM
                     GGHVRVGFEDNVYLEKGVLAKSNGELVARVARLSREIGREVATPDEARVILGLK"
     gene            2122..>3200
                     /gene="rho"
                     /locus_tag="PKK75_08030"
     CDS             2122..>3200
                     /gene="rho"
                     /locus_tag="PKK75_08030"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNZ83586.1"
                     /translation="MELGSLKLSELKELAKSKGMHDAYKYKKDELILKLTQFETSSPV
                     INEKGANEDIQEKEDTEQTAELPDKIVEEIETSGAEAIKVAGILETHPDGYGFLRTHN
                     YLTSDDDVYISPSQIRRFHMQTGDKISGITRPPKQGEKFKALLYVKEVNDLNPELARN
                     RKDFDTLIPVYPDQRIKLEISPIDIAMRIIDLIAPIGRGQRGMIVAPPKAGKTTILKN
                     MANSIAANYPEIEIIMLLIDERPEEVTDMQRNVKGDVVYSTFDELPKNHIKVAEMVLD
                     RAKRLVEHGKDVVILLDSITRLARAYNLTITPTGRTLSGGLDPGALYGPKRFFGAARN
                     IENGGSLTILASALIETGSRMDDLI"
BASE COUNT         1031 a          585 c          597 g          987 t
ORIGIN      
        1 ctgtcacttc ctatgttcat caagcctgcc aacaacggct cttccatcgg cataagcaaa
       61 gtgagaaact atgatgaatt tgaaaagggg attatggaag cctttgaata tgacaggaaa
      121 atagttcttg aatcaaatat agaaggattt gaaataggct gtgctgtggt aggtaacgac
      181 gagcttttaa tcggtgaggt tgatgaaata gacaccaaga atgacttttt tgactatgtt
      241 gaaaaatatt ctcagcacaa ttcaaaaata tattgtcctg cacgaatcag cgatgactta
      301 aaagcccggg caaaagaaat tgcagcaaag acctacaaag cattaggctg cagcgggctt
      361 tccagagtgg atatgttttt aacacctgaa aatgatatat atttaaatga aatcaatact
      421 atacccggac tgacggactt aagcaggtat ccttcaatgc ttaaaaaaat cggtattgaa
      481 tacaaggatt tgattgtaaa gcttgtagac ctggcaatgg aaagaagata gtagtgaaca
      541 gtgaaaaata aacaataaaa agttatgaaa ggatttgtat aaaagtacaa atccttgtta
      601 gttgccgttt ttaatgttaa ttttttcctg agcagccgct gtaaatattg tttgacctat
      661 attattgatg aattttccaa gtatgatttg gtcctttgca tcgatgccgt ctatcaaaaa
      721 aatgccgatc aaggttgata aaagtacaaa ttgcttgggc ggcaggttgg tgatgcatga
      781 aatcagtgcc ttgtatccag tgtttgtatt attttcgatt tctttatcat cagagctgtc
      841 aatatttagt tttctgatta taccttcaaa caccatactt tcctgatcca tacaacactt
      901 ccttttatct gttaaattaa tatatgtttt aagtcagtta aggttaccaa taaaaataag
      961 agaacggccg gccgttctct aaaatctctt ttctttttaa ttgtgtcagt cttaaagagc
     1021 atcccccagt ctccttcacc aaagtattcc atttggagag ggttagggca tctttatttc
     1081 agtcccagta ttacacgtgc ctcgtccggt gttgctacct cccgtccaat ttctctagaa
     1141 agacgtgcaa ctcttgctac taattctcca ttagatttag ctaatacacc tttttctaag
     1201 tatacattat cttcaaagcc aactcttacg tgtccaccca tagctattgt tgcagctgct
     1261 atggtccaag tacttttacc aataccagtt gcagtccatg ttgaaccttg cggtaaactt
     1321 ccaaccatga ataccaggtc acggatagtt gctgacattt gtactccaag aacaaagtca
     1381 aaatgtaatg gatgaactaa taaacctttt ctatcagctt ttaatgctat gtcaatcata
     1441 cctttgtcaa atacttcaag ctcaggtttg attccttttt ctttcataat cttagcgaag
     1501 ttaaaaattg tattatcagt atttataaat atttcatctc cgccaaagtt acatgtacca
     1561 cagtctaatg ttgcgaattc tggtgtaggt acgatgtctg tagaagctaa tctttctaag
     1621 tcagtcattc caactgctcc acctgtagaa ggttgtacta ttacatctgg acaaactgct
     1681 ttaattgcat caactgcttc ctggaatctc tttgtatctt gtgttggagt tccatcatcc
     1741 catcttacgt gaagatgtat aattgatgca ccagcatcat atgcagattt tgcttctctt
     1801 acgatttcct caattgtata aggaactgca gggttatgct cttttgttac ttcagctcca
     1861 caaattgcag ctgtgataat taatttttcc attttaacct ccatttataa ttttttgcct
     1921 acgattattt taccttatta attcaatata gtcaactgac tgttaaaaat atgtcatggc
     1981 tcttgcatat tatttcataa aataaaggat taatttattt tcttgactaa tatattataa
     2041 aatggtatta tttaggcact cttcgaaatc tttccggaat ttttccataa ttattattaa
     2101 ttaaatatgc agggaggtaa tatggagctt gggagtttaa agctttctga attaaaggag
     2161 ctggctaaat caaaaggcat gcatgatgcc tataagtata agaaagatga attaatttta
     2221 aaattaacac agtttgaaac aagcagcccg gttataaatg aaaagggcgc aaatgaagac
     2281 attcaagaaa aagaagatac agagcaaacg gcggaattgc ccgataaaat agttgaagaa
     2341 atcgaaacaa gcggcgcgga agctataaag gtagcaggca ttcttgaaac tcatcctgat
     2401 ggttacggct ttttaagaac tcataactac ttaaccagcg atgacgatgt atacatatcc
     2461 ccttcccaaa tcagaagatt tcacatgcag accggtgata aaatttcagg aataacaaga
     2521 cctccgaaac agggagaaaa gtttaaagca cttctttacg tgaaggaagt aaatgactta
     2581 aatcctgaat tggcaagaaa cagaaaggat tttgatactc ttattcccgt ttatcctgat
     2641 caaagaataa agctggagat atcacccata gatattgcaa tgaggattat tgacttaatc
     2701 gcacccatag gccggggtca aagaggtatg attgtagctc ctccaaaggc cggtaaaaca
     2761 acaattttaa aaaacatggc aaactccata gcagcaaatt atcctgaaat tgaaattatc
     2821 atgctcctaa tagatgaacg tcccgaagaa gtaacggata tgcagcgaaa tgtgaagggt
     2881 gatgttgttt attcaacctt tgacgagctg cctaaaaatc acattaaagt agctgaaatg
     2941 gtattagaca gggcaaaaag gcttgttgaa catgggaagg atgttgttat tctattagac
     3001 agcattacaa gacttgcaag ggcatataat ctgactataa ctcctacggg gaggaccttg
     3061 tccggcggac ttgatcccgg agcattgtac ggtcccaaga gatttttcgg tgctgccaga
     3121 aatatcgaaa acggcggaag tctcaccata cttgccagcg cacttattga aacaggaagc
     3181 aggatggatg atttaatatt
//
LOCUS       DAOPUT010000131         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_31176_length_3179_cov_22.677657, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000131 DAOPUT010000000
VERSION     DAOPUT010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31176_length_3179_cov_22.677657"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PKK75_08035"
     CDS             complement(<1..508)
                     /locus_tag="PKK75_08035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ83587.1"
                     /translation="MNPVVKFEFENGDIIKAELYPDIAPNTVKNFISLINKKYYDGKI
                     FHRVIKDFMIQGGCPNGNGMGGPGYSIKGEFNSNGFKNDLKHTEGVLSMARSMSPNSA
                     GSQFFIMHKRSPHLDGQYAAFGKVIEGMDVVDKIASTKTGRMDRPVSDVKLNKVTVDT
                     FGETYGEPE"
     gene            772..1731
                     /locus_tag="PKK75_08040"
     CDS             772..1731
                     /locus_tag="PKK75_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83588.1"
                     /translation="MKCSNNNDVLGTGGIADRCCTGQSGCDWDGTLRNVVTEPIYVQK
                     VYDAVLFNLQGLKTAPNTCFEPPLGCGTNIIRIADIRCRKFFNPDNIKDPCNLKINPN
                     TTLSGGQFVKKEDGCDFKVIGPDGTLSQKLVFADTRFCDEEELGTPIFGTQEIEVSGC
                     VEIEMDLVVSPCDSARESIITLTAVVEIANECNPLVLTNFFEICVPSVFDTAFLPRFT
                     EFCNLDCVIRLATNSIQRDFEIDPRTGVLSVDLIISLCISCEKKIVVPVQLCVLSTGF
                     VELSPEQAAICPTFPRLFPRQIDQNSVGGTGGGGCRPRSQSAE"
     gene            complement(1850..2047)
                     /locus_tag="PKK75_08045"
     CDS             complement(1850..2047)
                     /locus_tag="PKK75_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNZ83589.1"
                     /translation="MIIENNKKEKEKKQILLRLSSSLWEELAGWAEDDFRSINGQIEY
                     LLNECVKNRKRTKRDIGGVPK"
     gene            complement(2088..3095)
                     /locus_tag="PKK75_08050"
     CDS             complement(2088..3095)
                     /locus_tag="PKK75_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNZ83590.1"
                     /translation="MSDINKEKLITEEVLPNSMNGMFMLILNIALMLGSAILFVFSVS
                     SFGGGILKVVMIFISVLYFFIIGPVLFAGLKVLKPNEALVLTLFGKYYGSLKGEGFYF
                     VNPFVTATNPSGNVATTAVFSMEGTNKDKKNPELNIRSKKISLKTMTLNNDKQKINDA
                     LGNPITIGIVVIWRVVNTAKAVFNVDNYTEYLSIQCDSALRNIVRLYPYDTFGDDNEK
                     TLRGSSLEIANKLKEEIQEKVKIAGLEIIEAKITHLAYAPEIAAAMLQRQQASAVIDA
                     RQMIVEGAVGMVEMALAKLSENNVVELDEERKAAMVSNLLVVLCGNKDAQPIVNSGSL
                     Y"
BASE COUNT          898 a          618 c          570 g         1093 t
ORIGIN      
        1 tttccggttc cccgtatgtt tctccaaagg tatcaactgt gactttatta agcttaacat
       61 ctgaaacagg tctgtccatg cggcctgttt ttgttgaagc aattttatca accacatcca
      121 tgccttctat tacctttcca aatgctgcat attgcccgtc aagatgaggt gatcttttat
      181 gcatgatgaa aaattgcgac cctgcagaat tgggagacat ggaccttgcc attgacaata
      241 ccccttctgt atgctttaag tcgtttttaa acccgtttga attgaattct ccctttatgg
      301 aatatcctgg accgcccatt ccattcccgt ttgggcatcc tccctgaatc atgaaatctt
      361 tgattactcg gtgaaaaatt ttgccgtcat agtatttctt attgattaat gaaatgaaat
      421 tttttactgt gtttggagct atatccggat ataattccgc tttgattata tcaccgtttt
      481 caaactcaaa ttttactact ggattcattt tccctcctga aattttattc ttaatgatac
      541 tttacaataa caagagaaat aatacaaggg aaattccatt tagctctgaa ggagactttc
      601 ctatatacct ggatggtttg gcaggagcaa tcatgatcat cctaaagccg ctcataattt
      661 atattcaatt attgtcattt tttgtttttt aatttatatg tgtgtaacaa gtaaaagtat
      721 ttagaatata ttaaagtaaa gtaacagaaa ttatgaggag gttatgccag catgaaatgc
      781 tcaaataata atgatgtttt gggaactggc ggcattgcgg atagatgttg caccggtcaa
      841 tcaggttgtg actgggacgg tacattaaga aatgtagtga cagaacccat ttatgtacaa
      901 aaggtatacg atgcagtttt atttaacctg cagggattaa aaacagctcc aaatacttgt
      961 tttgaaccgc cgctaggctg cggaacaaat attatcagaa ttgcagacat aagatgcaga
     1021 aaattcttta atccggataa tataaaagat ccatgcaatt taaaaattaa tcctaatact
     1081 acattatcag gcggtcagtt tgttaaaaaa gaagatggct gcgacttcaa ggttataggt
     1141 ccggatggaa ccctaagcca aaagttggta tttgcggata caagattttg tgatgaagaa
     1201 gaattgggta cacccatatt tggaactcaa gagattgaag tttcaggatg tgttgaaatt
     1261 gaaatggatt tagttgtttc tccatgtgat tcagcgagag aaagtataat tacattaaca
     1321 gctgtagtag aaattgcaaa tgaatgcaat ccgttagtat tgactaattt ctttgagata
     1381 tgcgtgcctt cggtatttga taccgcattc ttgccaagat tcactgaatt ctgcaactta
     1441 gactgtgtaa taaggcttgc aactaacagt attcaaagag attttgaaat tgacccgaga
     1501 acaggagtat tgtctgtgga tctgattatt tcactttgca tctcctgtga aaagaaaata
     1561 gttgttcctg tacagctttg cgtgctttct actggttttg tcgaattgtc gcctgagcag
     1621 gcagctatat gccccacatt cccaagactg ttcccaagac aaatcgacca aaattcagta
     1681 ggcggaacag gcggcggcgg atgccggccg agatcccaat cagctgaata attaatacca
     1741 aatagctgaa ttttaaaaaa cccactatgt gtgggttttt aatttctgtg aagggacata
     1801 tgtccggctc ttgctaataa cctgatatta gaatttttat attactcaat tactttggga
     1861 cccccccgat gtctcttttt gttctttttc tgttctttac gcattcattg agtaaatatt
     1921 ctatttgccc gtttattgag cggaaatcat cctcagccca gcctgctaat tcttcccaaa
     1981 gagatgatga taaacgcagc aatatttgtt ttttttcttt ttccttttta ttattttcta
     2041 ttatcatcag ctcctttcta ttattcctgc actggccact tattttatta atacagcgag
     2101 cctgaattga ctatgggctg tgcgtctttg ttcccgcaaa gaacaaccag cagattgctt
     2161 accatggctg cttttctttc ttcatccaat tcgacgacat tattctcgct tagcttggca
     2221 agtgccattt caaccattcc cactgcacct tccactatca tctgtcttgc atcgattact
     2281 gctgaagcct gctgtctttg aagcattgct gcagctattt caggtgcata tgccaagtgg
     2341 gttatttttg cctcgattat ttcgaggcct gcaattttca ctttctcctg aatttcttcc
     2401 ttaagcttat tggctatttc caggctgctt cctcttagtg tcttttcgtt gtcgtcgccg
     2461 aatgtgtcat atggataaag tctcacaatg tttcttaatg ctgaatcgca ttgaattgac
     2521 aagtattcgg tatagttatc cacattgaaa actgccttag cggtattaac cactctccat
     2581 attacaacta ttccaatagt tatagggttt cccaaggcgt cgttgatttt ttgcttgtca
     2641 ttgttcagtg tcattgtttt taaagatatc tttttgcttc taatattaag ttccggattt
     2701 ttcttgtctt tatttgttcc ttccatagag aatactgcgg ttgttgcaac attgcccgat
     2761 ggatttgtag ctgttacaaa gggatttaca aaatagaatc cttcgccttt cagcgagccg
     2821 taatattttc cgaataaagt gagtacaagg gcttcattgg gctttaatac cttgaggcct
     2881 gcaaacaaaa caggacctat gataaaaaaa tacagcacgc ttataaaaat cataacaacc
     2941 ttcagtatgc cgccgccgaa tgaactcacg ctgaaaacaa acaaaatcgc cgagcctaac
     3001 atcaatgcaa tatttagaat cagcataaac atgccgttca ttgaattagg taagacttct
     3061 tcggtgatta atttttcttt atttatatct gacattttat ttcctcccaa taaatgtttt
     3121 ttaatatcat tttgatatca ttattatatt acacttgatt atatttgtca ataaaaact
//
LOCUS       DAOPUT010000132         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_31286_length_3171_cov_20.072850, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000132 DAOPUT010000000
VERSION     DAOPUT010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31286_length_3171_cov_20.072850"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..143
                     /gene="rpmJ"
                     /locus_tag="PKK75_08055"
     CDS             30..143
                     /gene="rpmJ"
                     /locus_tag="PKK75_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNZ83591.1"
                     /translation="MKVRPSVKPLCEKCKIIRRKGKVMIICENPRHKQKQG"
     gene            189..557
                     /gene="rpsM"
                     /locus_tag="PKK75_08060"
     CDS             189..557
                     /gene="rpsM"
                     /locus_tag="PKK75_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNZ83592.1"
                     /translation="MARIAGVDLPRDKRVEIGLTYIFGIGRPTSNKILKATGINPDTR
                     VKDLTEDEVQKLRTEIDKRPVEGDMRREVALNIKRLKEINCYRGLRHKRGLPVRGQNT
                     KNNARTRKGPKRVSGKKSKK"
     gene            572..973
                     /gene="rpsK"
                     /locus_tag="PKK75_08065"
     CDS             572..973
                     /gene="rpsK"
                     /locus_tag="PKK75_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNZ83593.1"
                     /translation="MAAKKQQKATRVKRRERKNIEKGQAHIKSTFNNTLVTLTDLQGN
                     AISWASSGGLGFKGSRKSTPYAASMVAEEAAKKAMEHGLKTVEVYVKGPGSGREAAIR
                     SLQAAGLEVNLIKDVTPIPHNGCRPPKRRRV"
     gene            992..1615
                     /gene="rpsD"
                     /locus_tag="PKK75_08070"
     CDS             992..1615
                     /gene="rpsD"
                     /locus_tag="PKK75_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNZ83594.1"
                     /translation="MARYTGPVCRICRREGLKLYLKGDRCYTDKCSIGRRNYAPGQHG
                     QNNKKLTNYGTQLREKQKVRRYYGVLEGQFTEYYNMAEKMSGKTGDNLLKLLELRFDN
                     VIYRLGFASSRPEARQLVVHGHFTVNGKKADIPSMILQVGDEIKVKEKSQNSDKFKVM
                     AENHKNTAPQWLQVEPENYTGRIIAEPAKEDIEIPIEEHMIVEFYSK"
     gene            1668..2615
                     /locus_tag="PKK75_08075"
     CDS             1668..2615
                     /locus_tag="PKK75_08075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNZ83595.1"
                     /translation="MIEIEKPNITLNEKNDNNTYGKIVVEPLERGYGTTIGNSLRRIL
                     LSSLPGAAVTSINIQGVLHEFSTIPGVREDVTEIILNIKNLAAKMNVQGPVQLLIDAK
                     GPKKVTAADIITGGDVDIINEDLHIATLEEGSELIIEMEMEQGRGYVIAERNKKDSQA
                     IGVIPIDSIYTPVKKVNFTVEDTRVGNRTDFDKLTIEVWTNGTIEPEEAISLGAKIMS
                     EHLNLFISLTEHVNEVEIMVEKEEDEKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LTYKTEEDMMKVRNLGKKSLDEVSKKLEELGLSLRKNDN"
     gene            2634..2978
                     /gene="rplQ"
                     /locus_tag="PKK75_08080"
     CDS             2634..2978
                     /gene="rplQ"
                     /locus_tag="PKK75_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNZ83596.1"
                     /translation="MGSHRKLGVKSDHRMAMLRNMTADLIMQGRITTTVTRAKELRRV
                     AERTITLGKRGDLHSRRRALAYLYDKDAVYKLFEEVAPKYKDRNGGYTRILKLGPRRG
                     DGAEMAIIELVE"
BASE COUNT         1266 a          462 c          720 g          723 t
ORIGIN      
        1 catggaggaa aaagtaagga ggtattacaa tgaaagtaag accatcagta aaaccactgt
       61 gcgaaaagtg caagattatc agacgaaaag gtaaagttat gataatttgc gaaaatccaa
      121 ggcacaaaca aaaacaaggt taggataaat aaaaaactaa gcactactaa ggtaggaggt
      181 gtaacttaat ggccagaatc gcgggcgttg atttgcccag agataagaga gttgaaatag
      241 gcttgactta tattttcgga ataggcagac caacatcaaa taagatttta aaagcaacag
      301 gcataaatcc tgatacaagg gttaaggatc taacagaaga tgaagtacaa aaattaagaa
      361 ctgaaataga taagcgtcct gtagaaggag atatgagacg tgaagtagca ttgaatataa
      421 aaagacttaa agaaatcaac tgctacagag gattaagaca taaaagagga ttgcctgtca
      481 gaggacaaaa tacaaagaat aatgcaagaa caagaaaagg tcctaagaga gtttcaggaa
      541 agaaatctaa aaagtaaaag gaggggctta aatggcagct aaaaaacaac aaaaagcaac
      601 gcgagtaaaa agaagagaac gtaaaaatat agaaaaaggc caggcgcata ttaagtctac
      661 ctttaacaat acacttgtta ctcttacaga cttgcaggga aatgcaattt catgggcaag
      721 ttcaggcgga ttaggattta agggttcaag aaagtctacc ccgtatgcag cgagcatggt
      781 tgctgaagaa gcagcaaaaa aagctatgga gcatggatta aaaacagtag aagtatatgt
      841 taaaggaccg ggctcaggaa gggaagcagc gataagatcc ctgcaggcag ccggacttga
      901 agttaattta ataaaagacg taacaccaat accgcataat ggttgcagac cgccaaagcg
      961 cagaagagta taataggaac gaggtgaaaa gatggcaaga tataccgggc ctgtctgcag
     1021 aatatgcaga agagaaggtt taaagttata tttaaaaggt gacagatgtt atactgataa
     1081 atgttcaatc ggcagaagaa attatgctcc cggtcaacat ggtcaaaata acaagaaact
     1141 gactaactat ggaactcagt taagagaaaa acaaaaggtc agaagatatt acggagttct
     1201 ggaaggacaa tttaccgaat actacaatat ggcggaaaaa atgtccggaa aaaccggtga
     1261 taatcttttg aaattgcttg agcttagatt tgacaatgta atatacagat taggctttgc
     1321 aagttcaaga cctgaagcaa gacaattggt agttcacggt cattttacag taaacggcaa
     1381 gaaggctgat attccgtcaa tgattttaca ggtaggcgat gagatcaagg ttaaggaaaa
     1441 gagccaaaat tcagacaaat ttaaagttat ggcagaaaat cacaagaata ctgcacccca
     1501 atggctgcag gttgaacctg aaaactacac agggagaata attgccgaac ctgctaaaga
     1561 agatatcgaa ataccaatag aagagcatat gattgtcgag ttctactcca agtaatggca
     1621 tttaagaatt attaccctcg acaaacaaaa aaataaggag ggttgggatg atagaaattg
     1681 aaaaaccaaa tattacttta aatgaaaaga atgacaacaa cacctacggt aagatagttg
     1741 ttgaacccct tgaaagaggc tatggaacaa caatagggaa ttcattaaga agaatacttc
     1801 tttcatcgtt accgggagca gctgttactt caataaacat tcaaggtgta ttgcatgaat
     1861 tttctacaat acccggagtg agagaagatg tgacagaaat aattcttaat attaagaact
     1921 tagctgctaa aatgaatgtg cagggacctg tgcagctttt aattgatgca aaaggaccca
     1981 agaaagttac ggcagcagat ataattacag gcggagatgt tgacatcata aatgaagacc
     2041 tgcatattgc aactcttgaa gaaggctctg aactcattat tgaaatggag atggagcagg
     2101 gaagagggta cgtaatagcg gaaagaaaca agaaagacag ccaggcaatc ggagttattc
     2161 ctattgattc aatatacaca cctgtaaaaa aagtaaactt tacggttgaa gatacaagag
     2221 tgggaaacag gacagatttc gacaagctta ctattgaggt ctggaccaac ggaacaattg
     2281 agcctgaaga agcaatttct ttaggagcca aaataatgag tgaacacctc aacttattca
     2341 tatcattgac agaacatgta aatgaagtgg aaatcatggt tgaaaaagaa gaggatgaaa
     2401 aagaaaaagt tttggaaatg acaatagagg aacttgattt atcggtaagg tcgtacaatt
     2461 gtctgaagag ggcaggaata aatactgttg aggagcttac ttacaagact gaagaagaca
     2521 tgatgaaagt aaggaacctg gggaaaaaat ctttggatga agtatctaag aaacttgaag
     2581 aattaggttt atctctaagg aaaaatgata attagcttta aaaggaggac taaatgggta
     2641 gtcacagaaa gcttggggtt aaatctgacc acagaatggc aatgcttcga aatatgactg
     2701 ctgaccttat tatgcagggc agaataacga caacagttac aagagccaaa gagctcagaa
     2761 gagttgcaga gagaactatc actctgggga aaagaggaga cctgcattca aggcgtcggg
     2821 cattagctta tctatacgac aaagatgcag tatataagct gttcgaagaa gttgctccta
     2881 aatacaaaga tagaaacggt ggatacacca ggattctaaa attgggcccc agaagaggcg
     2941 atggagcaga aatggcgata atagaattag ttgaataaat atcggggacg attcttttaa
     3001 agaaccgtcc cttgttttgt ggatataaaa acagcaagta aaattaatgt tgtaaaataa
     3061 tgtgaataat gttattattt ttatacggct ttaacgttag aatttaaata acaggtgaaa
     3121 aatggacgat tacataataa agatagacaa tgtaaaattt aaatacgaaa a
//
LOCUS       DAOPUT010000133         3166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_31360_length_3166_cov_20.280296, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000133 DAOPUT010000000
VERSION     DAOPUT010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31360_length_3166_cov_20.280296"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKK75_08085"
     CDS             <1..408
                     /locus_tag="PKK75_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNZ83597.1"
                     /translation="HGSAVFIGCLISNITDEYILQLFAGVIFIIFGIITLVFEDEGEN
                     KKLYSLGPVITVALTFFLGEMGDKTQLTCMTLSMDAVYPLIVLAGSVTAMVSIGFIGI
                     IVGTSLTKFLPSYIIKTISGLIFIIFGIMRLIV"
     gene            408..1046
                     /locus_tag="PKK75_08090"
     CDS             408..1046
                     /locus_tag="PKK75_08090"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNZ83598.1"
                     /translation="MNIKNIKDKLKNTKPKPMDYEVSFAVLVPLIEIDGELNLIYEVR
                     SNSIEQPGEISFPGGRIEDGESPEEAAIRETSEELLLNKANIEIITELNYASSKSGAF
                     IFTFLGLIKDIDPYEIGYSIDEVSEVFFVPLSFFLETSPEKYYMNYYPKADKDFPYHM
                     VNNGEDYNWGSIRYPVYFYKYYNYIIWGLTAKITYSFINELIGDDKEGKSES"
     gene            1036..1932
                     /locus_tag="PKK75_08095"
     CDS             1036..1932
                     /locus_tag="PKK75_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNZ83599.1"
                     /translation="MKANIFNIQKFSVHDGPGIRTTVFFKGCPLKCAWCHNPESQNAG
                     MEILYDKNKCTLCGNCIKACQNNAIEIKNNILTTNMDKCTFNGDCIVCCINSARQIAG
                     KEYTVDEVLKEVTKDRIFYKNSRGGVTLSGGEPLIYAKFVEELLMKLKEENIHTAVDT
                     CGYGDFKTIEKISKYTDLFLYDIKLMEDKEHILYTGVSNAIIIDNLRRLSITHNNIHL
                     RLPLIEGINADEDNILKILKLIKDINIKKINLLPYHDIAMHKYEKLGRRYSDSMKRPA
                     DEKLKRFREIFEKEGYKVKIGG"
     gene            1934..>3166
                     /locus_tag="PKK75_08100"
     CDS             1934..>3166
                     /locus_tag="PKK75_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNZ83600.1"
                     /translation="MRGYTERTKKLREISTRTQPSISLERALIETEFYEKFYGTVEIP
                     VLRALNFKNLMEKRKLYIGEGELIVGEKSEGPQVAPTFPELCCHTVEDMTVMNDRKYI
                     SFKVKEEDKILQQGKIIPYWENRSIRHKILEAMTQEWKDCYAAGMYTEFMEQRAPGHT
                     VADGKIYEKGFLDFKKEIEEEIEKLDFINDPDAYDKKAQLQGMAISCDAIIIYGKRYA
                     QYARDLALKEENPARKEELLWIANNCDIVPAHKPETFAQALQMYWFVHVGITTELNTW
                     DSFSPGRLDQYIYPFYKKEKEEGTIDYSKARELLECFWIKFNNQPAPPKVGITLKESG
                     TYTDFANINTGGINPCTGEDGVNEISYMILDTMDEMKLLQPSSNVQISEKTPHEFLKR
                     AVEISRKGWGQPAFYNTEE"
BASE COUNT         1146 a          519 c          665 g          836 t
ORIGIN      
        1 cacggctctg cagtttttat aggctgtctc atatccaata ttactgacga atatattctt
       61 cagttatttg caggtgttat tttcataatc ttcggaatca ttacccttgt atttgaagat
      121 gagggcgaaa ataagaagct ctacagcctg ggacctgtta ttaccgtcgc tcttacattt
      181 ttcctcggag aaatgggaga taaaacccag cttacatgca tgaccttgtc catggacgcc
      241 gtatatcctt taatagtcct ggcaggctct gtaactgcaa tggtttccat aggtttcata
      301 ggaattattg taggaacatc attgactaaa tttcttcctt cttatataat aaaaacaata
      361 tcagggctga tatttataat attcggaata atgaggctga tagtataatg aatatcaaaa
      421 acataaaaga caagctaaaa aatacaaaac caaaaccaat ggactacgag gtaagctttg
      481 cagtattggt tcccttaata gaaattgacg gagaattaaa cttaatttat gaggtcaggt
      541 caaattctat cgagcagccg ggagaaattt cttttccagg cggtagaatc gaagatggtg
      601 aaagccccga ggaagcggca ataagggaaa cttcagagga gcttttatta aataaagcaa
      661 acattgagat aattacagag cttaattatg cttccagcaa aagcggtgct tttattttca
      721 catttttggg actgattaag gatattgacc catatgagat aggatacagc attgatgagg
      781 tttcggaagt tttctttgtt cccttgtcat tctttttgga aacctctcct gaaaaatact
      841 atatgaatta ttatccaaag gcagataagg attttccata tcatatggtt aataacggtg
      901 aggattacaa ctggggaagc ataagatatc ctgtttattt ttacaaatac tacaattata
      961 taatatgggg cttgactgct aaaataactt acagcttcat taatgagctt ataggtgatg
     1021 acaaagaggg aaaaagtgaa agctaatata ttcaacattc aaaaattttc cgtccatgac
     1081 ggaccaggca taagaacaac agtatttttt aaaggatgtc ccctaaagtg cgcatggtgc
     1141 cacaatcctg aaagccaaaa tgcaggcatg gaaatcctgt atgataaaaa caaatgtact
     1201 ttatgcggaa attgtataaa ggcatgtcaa aacaatgcca tagaaataaa aaacaatatt
     1261 cttacaacga atatggacaa gtgtacattt aacggggact gcattgtctg ctgcataaat
     1321 tctgccaggc agattgccgg aaaagaatac acggtggatg aagttttgaa agaagttaca
     1381 aaagacagaa tattttataa aaactccagg ggaggcgtga ccttgtcagg gggagagccc
     1441 ctgatttatg caaagtttgt tgaagagctt ctgatgaaat taaaagaaga aaacattcat
     1501 actgcagtag acacctgcgg ctatggagac tttaagacaa tagaaaagat atcaaaatat
     1561 acggatttat ttctatatga cattaagctc atggaagata aagagcatat actctataca
     1621 ggagtttcaa atgcaatcat tatcgacaat ttaagaagac tgtcaataac tcacaacaac
     1681 atacatttga ggctgccttt aattgaaggt attaatgccg atgaagataa tatcctaaag
     1741 atattaaagc taattaagga tataaatatt aagaaaatta atctgctgcc ctatcatgac
     1801 atagcgatgc ataaatacga aaaattagga cgcaggtatt ctgattccat gaaaagacct
     1861 gcagatgaga aacttaaaag attcagggaa atattcgaaa aagaaggata taaggtcaaa
     1921 ataggaggtt aaaatgagag gatatactga aagaacaaaa aaattgaggg aaataagcac
     1981 aagaacacaa ccgtccataa gcttggaaag ggctttaata gaaactgaat tttatgaaaa
     2041 attttacggt acagttgaaa tccctgtatt gagggcattg aattttaaaa acttaatgga
     2101 aaagagaaag ttgtacattg gcgaaggtga acttatagtg ggagaaaaaa gcgaggggcc
     2161 tcaggttgcc cccacattcc cggagctttg ctgccacact gtggaagata tgaccgtaat
     2221 gaatgacaga aaatatataa gctttaaagt taaggaagaa gataaaatcc tgcaacaggg
     2281 aaaaattatt ccatattggg agaaccggtc cataaggcat aaaatacttg aagccatgac
     2341 gcaggaatgg aaggactgct atgctgcggg aatgtatact gagttcatgg aacaaagagc
     2401 tcccggacat acagttgcag acggtaaaat ctatgaaaaa ggattccttg atttcaagaa
     2461 agaaatagaa gaagaaatag aaaagcttga tttcataaat gacccggatg catacgacaa
     2521 aaaggcacag cttcaaggga tggctatttc atgtgatgca ataattattt acggcaaaag
     2581 atatgcacag tatgcgagag acctggcttt aaaggaagaa aacccggcaa gaaaagaaga
     2641 attgctatgg attgcaaata actgtgacat agtgcctgct cacaagccgg aaacttttgc
     2701 acaggccttg cagatgtatt ggtttgtgca tgtaggcata accactgagc ttaatacctg
     2761 ggattcattt tctcccggca ggcttgacca gtatatatat cctttctata aaaaggaaaa
     2821 ggaagaaggg accattgatt actctaaggc aagggagctt ttagaatgct tttggataaa
     2881 attcaataac cagcctgctc ccccaaaggt aggaataacc ctgaaggaaa gcgggactta
     2941 cacggacttt gcaaatatta acaccggagg tatcaacccc tgcacgggag aagacggtgt
     3001 aaatgaaata tcttatatga tacttgacac aatggatgaa atgaagcttc ttcagccaag
     3061 ctccaatgtt cagataagtg aaaaaacacc ccatgaattt ttgaaaagag ctgtggaaat
     3121 ttcaagaaag ggctggggtc agccggcatt ttacaatacg gaggaa
//
LOCUS       DAOPUT010000134         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_31904_length_3129_cov_16.639232, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000134 DAOPUT010000000
VERSION     DAOPUT010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31904_length_3129_cov_16.639232"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKK75_08105"
     CDS             complement(<1..341)
                     /locus_tag="PKK75_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNZ83601.1"
                     /translation="MDNKDYLTRIENNRELMKSVFTERGHKSDQQSQVPQPPLAKESV
                     NENKMVLTKDFSGLIKESNVLTIINERKSHRVFKKESLTLDHLSFLLWASQGVKGIKG
                     DNYATERTVPSG"
     gene            complement(362..940)
                     /locus_tag="PKK75_08110"
     CDS             complement(362..940)
                     /locus_tag="PKK75_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83602.1"
                     /translation="MSNLVTSIGIIAGIFYIIYVIMDFMADNFIYEKQNILLMLVMAI
                     LMNIAGFMHSRYYYYDKTSTMLVNIAVVTALLGGYLVFQRKRIYYFRGIDNKLINKYK
                     NEITKIIEDYKADNLGRKADINFINNKLIFSKVSKDKTKKCLSLIGTFLDENRREYKL
                     KDYLAYFIRGHLFPAVTAILIFFIFYKLMQLI"
     gene            1336..1923
                     /locus_tag="PKK75_08115"
     CDS             1336..1923
                     /locus_tag="PKK75_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6143 family protein"
                     /protein_id="HNZ83603.1"
                     /translation="MNSHILYNQALHAGALNTNTSLMNYSVNIPISLMKSIEGKYFVG
                     IAPNLQFGNATKAWARLYNPPNSGVNLFVNTWTVSDIFSTPYSVQIWFNSTPPGLIQF
                     SRSVTPSNLAISPPPQSNIQLQYAISVSGFPAVGVKAYSRYGLAGTTINSEEEGKFIF
                     APGGSYLIFLSNPERPTIPASGNIAFGWWEEPIYQ"
     gene            complement(2104..2364)
                     /locus_tag="PKK75_08120"
     CDS             complement(2104..2364)
                     /locus_tag="PKK75_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNZ83604.1"
                     /translation="MFLSRRILYLGDHHNNDKPSKKYSDMFAALAAFSQIGFTIAVSV
                     LGGVLLGQYLDDKFGTDPWFLLAFSILGVGVGIRALFNIAKK"
     gene            complement(2570..>3129)
                     /locus_tag="PKK75_08125"
     CDS             complement(2570..>3129)
                     /locus_tag="PKK75_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ83605.1"
                     /translation="VFNEKTITLKNGERCLLRSPEAKDAAEMLNYLKKSAAETDFLLR
                     YPEEVVMTIEEEVNFINFLREAPRGVMICAFINNKLVGTTHLMAIGERFKIFHRASFG
                     IAVLKEAWHIGIGNALITEILKIAEDIGFELVELEVASPNHNAIGLYKKFGFKIYGTR
                     ENGFKFKDGTYCDEHLMMRKIGSRI"
BASE COUNT          937 a          613 c          451 g         1128 t
ORIGIN      
        1 cctgagggga cggttctttc tgttgcataa ttgtctcctt tgattccctt taccccttga
       61 ctagcccaca ataaaaatga caggtggtct aatgtaagac tttctttttt aaaaacccgg
      121 tggctttttc tttcatttat aatcgttaaa acatttgact ccttaataag tcctgagaag
      181 tctttagtaa gtaccatttt gttttcgttg actgattctt tagctaaggg tggctgaggc
      241 acctgactct gctggtctga cttatgtcct ctttctgtaa atacactttt cattaattct
      301 ctgttgtttt ctattcttgt taagtaatct ttattatcca tataataatc ctttctttat
      361 tttatattaa ctgcatcaat ttataaaata taaaaaatat taaaattgca gtaactgcag
      421 ggaaaaggtg tcctctaata aaatatgcca aatagtcctt taatttatat tccctcctgt
      481 tttcatccaa aaaagttccg attagagata agcatttttt agttttgtct ttgcttactt
      541 tactgaatat taatttgtta ttgataaaat taatatcagc ctttctacct aaattatctg
      601 ctttgtaatc ctcaattatt tttgttattt catttttata tttattaatt agtttattgt
      661 ctattcccct gaaataatag attctttttc tttggaaaac caaatagccg cccaatagtg
      721 cagtaaccac tgctatattg accagcattg ttgaagtttt gtcataataa tagtaacggc
      781 tgtgcataaa gcctgctata ttcatcagta ttgccattac taacattaac aagatattct
      841 gtttttcata tatgaagttg tcagccataa agtccattat cacgtagata atataaaaga
      901 ttcctgctat tattcctatt gatgtcacta aattactcat atcttcaacc cttccttacc
      961 ttgcctcttt tttgggacgg ttcttttgtt cttaaaagat tattacgctt aatttaggat
     1021 gtttatttct gtcgtctcta attaatttta aggttattca tactaaagac gcaaacagct
     1081 ttctgttaat aattacatta taccacatca aatgactaaa ttaacataaa attgttcctt
     1141 atttcaataa aaaacgggta ctagcgcgtt cccgttactt aatttaatat aagattattc
     1201 ttttaacgtt tagaatcgtt cccaaagtgt ataccaaaag gagtcttgac caaaccatcc
     1261 ctaaaactct gaattcacct ttcatatact tctatcatat aataaataac tatgatatga
     1321 aaggttgcaa atttaatgaa tagccatata ttatataacc aagctctaca tgcaggagct
     1381 ttaaatacta ataccagctt gatgaattac agtgttaata ttcctataag cttaatgaag
     1441 tctattgagg gaaagtattt tgtcggaatt gccccaaacc tgcaatttgg caatgccacc
     1501 aaagcatggg caaggctata caatccgcca aattccggtg tcaatttatt tgtaaatact
     1561 tggactgtaa gtgatatctt ttcaacacca tatagtgtac aaatttggtt taacagtaca
     1621 ccgccgggat taatccaatt ttcccgatct gttaccccat ctaatttggc aatttctccg
     1681 cctccacagt ctaatataca gcttcaatat gctatttcag tcagtggttt tccagcagta
     1741 ggagttaagg cttacagcag atatggtctt gccggtacta ccataaattc agaggaagaa
     1801 ggaaagttta tatttgctcc aggcggttca tatttaattt ttttatcaaa ccccgaaaga
     1861 ccaactatac ctgcttcagg taatattgca ttcggatggt gggaggaacc aatttatcaa
     1921 tagccgctta ttgtttaact gaaaattatt cttatataaa ttatgattgg tatgtctgtg
     1981 tcgccatact tcttttgttg tgtctacgtt tctttgagaa aacatcctga aaaaaatctg
     2041 agaacgaaaa aaaccgtccc caaaatactt ctccacttcc cgtaatctta cgatttgtca
     2101 tttttatttc ttggcaatat taaataaggc cctgattcca accccaacgc ctaaaataga
     2161 aaaagccaaa agaaaccatg ggtctgtacc gaatttgtcg tctaaatatt gtcccagtaa
     2221 taccccgccc agtaccgata ctgctattgt aaatcctatc tgtgaaaagg cagctaaggc
     2281 agcgaacatg tcactgtatt tttttgatgg tttatcatta ttgtggtggt cgcccaaata
     2341 taggatcctc ctcgataaaa acatagtgtc ctgttgaaat aagtcggaca ccatttttgc
     2401 tctctaaaat cttaacatat atgtcgttat aatgtcaagt tttagaatct gttcatgcaa
     2461 taaaacatag gattcttcgt tttccgaaag gtggtcagga aggtatattt tgaatcatcg
     2521 cctgccatat ctacttcctg ttcatatgaa attttaccag taatatattt taaatcctgc
     2581 ttcctatctt tctcatcatc aaatgctcgt cacaatatgt gccgtcctta aatttaaagc
     2641 cgttttccct tgtgccgtat attttaaatc cgaatttctt ataaagccct attgcattgt
     2701 gatttggact tgcaacctcc agctcaacca gttcgaaacc gatatcctct gcaatcttta
     2761 atatttcagt tatcagggcg ttccctattc caatgtgcca tgcttccttt agaaccgcta
     2821 tgccaaaaga tgctctatga aagattttaa acctttcacc gatagccatt aagtgagtgg
     2881 ttccaacaag tttattatta ataaaagcac aaatcattac acctcttgga gcttccctta
     2941 aaaaatttat gaaatttact tcttcttcaa ttgtcattac tacttcttca ggataacgaa
     3001 gcaaaaaatc cgtttctgct gctgacttct ttaaataatt aagcatttca gctgcatctt
     3061 tagcttcggg gctccttaat aaacatcttt caccattttt taatgtaata gttttctcat
     3121 tgaatacca
//
LOCUS       DAOPUT010000135         3048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_33117_length_3048_cov_17.268293, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000135 DAOPUT010000000
VERSION     DAOPUT010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33117_length_3048_cov_17.268293"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..144
                     /locus_tag="PKK75_08130"
     CDS             16..144
                     /locus_tag="PKK75_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ83606.1"
                     /translation="MCLRSRCPAIKITGKAEIDKEKCKGCGICVSLCLTEAIVEIK"
     gene            155..703
                     /locus_tag="PKK75_08135"
     CDS             155..703
                     /locus_tag="PKK75_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020064181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ83607.1"
                     /translation="MNHLGTKEIETDRLLLRKFKLSDAEEMYKNWANDDNVTRYITWP
                     THKDIEETRRVVEGYVNDSARDNYYHWCIVLKETNEAVGSIGAFRLYDDLKLFEIGYC
                     IGKKFWNKGITTEAMKALIKFFFEEVGVNRIEARHDVKNPASGRVMIKSGLKFEGILR
                     QAGKTNNGINDMAVYAILKEEW"
     gene            883..1554
                     /locus_tag="PKK75_08140"
     CDS             883..1554
                     /locus_tag="PKK75_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HNZ83608.1"
                     /translation="MTKIMRIFLIAVVESIIMSTAVFAAEAEPNKKGLVMIPLMNIRI
                     SPSNEAESIGKLFFGTTVEIMSETEDWYEICYKSKTAYICAEFVKIFEIIDNSVNTGA
                     SIVEYAKTFIGTPYASGGNSPRGFDCSGYIQYVMKNFGVDMPRTSTDQYSIGVRVSKS
                     ELMPGDLVYFKYSASSSRLSHVGIYIGDGNFIHSPVPGQSVRVSPLNAGYFSCYYYGA
                     TRVLQ"
     gene            complement(1645..2049)
                     /locus_tag="PKK75_08145"
     CDS             complement(1645..2049)
                     /locus_tag="PKK75_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNZ83609.1"
                     /translation="MKRFLIVLLIVIVITIIFTGCTAKDNNQPGTENSYIKISAEEAK
                     EIIDSEDVIVLDVRTQEEYNEGHIENAVLLPVNDIAVKAEEVLTDKDAKILVYCRSGN
                     RSAAASKELIKMGYTNVFDFGGIISWPYEIVK"
     gene            2251..3042
                     /locus_tag="PKK75_08150"
     CDS             2251..3042
                     /locus_tag="PKK75_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ83610.1"
                     /translation="MDKITIVEFATEFLLNDLNHVSRELAIRDELAGMKIFNEPIFAY
                     GDADDNGFQSLKETTVVGEHFMLPKEWLPEAKTVIAYFFPFTDEIIESNRMDMLWPSN
                     EWLHGRIEGQNFLLEFSRFLYSKLNDAGYKSLVPGLDHRFKTKGFTSNWSERHVSYVC
                     GLGTFGLSKGIITEKGMAGRLGSIITELKLEPGIKKYKDVYEYCTLCGNCAINCPVDA
                     ISVEEGKDHRKCSDFLDTVREKHKTRYGCGKCQVSVPCEKGIPRG"
BASE COUNT         1074 a          447 c          605 g          922 t
ORIGIN      
        1 tgcagggcat gtaaaatgtg cttaagaagc aggtgccctg ccataaaaat aaccggcaag
       61 gctgaaattg ataaagaaaa atgcaaggga tgcggcattt gtgtcagctt gtgtttaaca
      121 gaggctatag tggaaattaa atagggggca cattatgaat cacctgggta ctaaagaaat
      181 tgaaacggat aggctgcttt tacgaaaatt taaattatca gatgcagaag aaatgtataa
      241 aaactgggca aatgatgata atgttaccag atatataacc tggcccactc ataaggatat
      301 agaagaaacc agaagggttg tagaaggata tgtcaatgat tccgccaggg ataactatta
      361 tcattggtgc atagttctaa aggaaacaaa tgaggcagta ggaagtatcg gggcattcag
      421 gctgtatgat gatttaaaat tattcgaaat cggatactgc attggcaaaa aattttggaa
      481 taaaggtatc actacagaag caatgaaagc cttgataaag tttttctttg aagaggtggg
      541 agtaaataga attgaagcaa gacatgatgt taaaaaccct gcatcaggaa gagttatgat
      601 taaatcggga cttaagttcg aaggtatatt aagacaggca ggcaaaacta acaacggaat
      661 taatgatatg gcagtttatg caatattaaa agaagaatgg taagtaatgg tgaatactcc
      721 cgagctaagt tcgaatatgt ttgcaagcat gttttgtttg tcataattaa gatttttgat
      781 accttgtatt aattgcttcc acatatcaaa tcgagaaatt gcgtggtttt attaacctta
      841 aaatcagaaa ggaatataat ttaatatata agggatgaga ctatgacaaa aatcatgagg
      901 atttttttaa tagcagtcgt tgagtcaatt attatgtcaa ctgcggtatt cgctgcagaa
      961 gctgagccaa ataaaaaagg attagttatg attcctttaa tgaatataag aataagccct
     1021 tcaaatgaag cagaaagtat tggcaaactg tttttcggaa caacagttga gataatgtct
     1081 gaaaccgaag actggtatga aatatgctac aaatcaaaaa ctgcatatat ttgtgctgaa
     1141 ttcgtcaaga tttttgaaat tattgataat tcagtaaata ccggggcaag tatcgtggag
     1201 tatgcaaaaa catttatagg aactccatat gcatcaggag ggaattctcc aaggggattt
     1261 gactgttcag gatatatcca gtatgtgatg aaaaatttcg gagtcgatat gccaagaaca
     1321 tcaacagacc agtattcaat aggtgtgaga gtttcaaaat ctgagcttat gcccggtgat
     1381 ttggtatatt tcaagtattc agcaagctcc tccagattaa gccatgtggg aatttatata
     1441 ggtgacggaa atttcattca ttcaccggtt cccggccaat ctgtgagagt cagtccgcta
     1501 aatgcaggat atttttcatg ttattattac ggagcaacaa gagtattgca ataaataatt
     1561 acaagcagtc agcaatacta gatgcttttt tgctgtatat ttacaaacac caccgattta
     1621 gctggtggtg ttttataaat attgttattt aacaatttca tatggccagg agattattcc
     1681 tccaaaatca aaaacatttg tatatcccat tttgattaat tcttttgatg ctgcagcgct
     1741 tctgtttccg cttctgcagt atacaagtat ctttgcatcc ttatcagtga gaacctcttc
     1801 agctttaact gcaatatcat ttacaggcag caaaacagca ttttctatat ggccttcatt
     1861 gtattcttcc tgagtgcgaa cgtctaaaac aataacatct tcactgtcaa tgatttcttt
     1921 tgcttcttca gcactgattt ttatatatga attttcagta cctggttgat tgttgtcctt
     1981 cgctgtgcat cctgtaaata ttatggttat tacaattact attaatagta caatcaaaaa
     2041 tctcttcata atatatcctt cctaaaatta aatatataaa aatattaccc ataaaaaata
     2101 gtttaaaaca attataatag tattataact ttatttgaat ttcaataaca cttaatcagg
     2161 gaaatattgt ataataaggg tatatcttat gctttaataa gtcaaataag tttaataaaa
     2221 aataatttta ataaatttag gggtgaaaat atggacaaaa taacaatcgt agaatttgca
     2281 actgaatttt tactaaatga cctcaatcat gtatcaagag aattagcaat aagagatgag
     2341 cttgcaggaa tgaaaatatt taacgagccg atatttgctt atggggatgc agatgacaat
     2401 ggattccaat cattgaaaga gaccactgtt gtaggtgagc attttatgct gccaaaggaa
     2461 tggcttccag aagcaaaaac agttattgct tatttctttc catttacaga cgaaataata
     2521 gaaagcaaca gaatggatat gctctggccg tcaaatgaat ggctgcacgg acgtattgaa
     2581 ggtcagaatt ttttattaga attcagcaga ttcttatatt ccaaattaaa tgatgcaggt
     2641 tataaaagcc ttgtcccggg attagaccat agattcaaaa caaagggttt caccagcaac
     2701 tggtcagaga ggcatgtatc ttatgtatgc ggccttggaa cctttggctt gtcaaaagga
     2761 ataattacag aaaaaggaat ggcaggaaga ttgggaagta ttataacaga attaaaatta
     2821 gaaccaggca taaaaaaata taaggatgtg tatgaatatt gcacattgtg tggtaattgt
     2881 gctattaatt gccctgttga cgcaatatct gttgaagagg gaaaagacca caggaaatgt
     2941 tctgattttt tggatacagt cagagaaaaa cataaaacac gttacggctg cggaaaatgt
     3001 caggtatctg ttccctgtga aaagggaata ccaagagggt aattctga
//
LOCUS       DAOPUT010000136         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_33697_length_3014_cov_17.812437, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000136 DAOPUT010000000
VERSION     DAOPUT010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33697_length_3014_cov_17.812437"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..778)
                     /locus_tag="PKK75_08155"
     CDS             complement(65..778)
                     /locus_tag="PKK75_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ83611.1"
                     /translation="MVNGEDMKELNNIIELKNINKIYGEKIKTQVLFDINISFEENSF
                     NSIIGESGSGKSTMLNIIGTLDKPTSGEVYIGGKRTDTMDKTELAFLRNKTIGFIFQF
                     HYLLPEFTAKENVLLPYKIHNYKVPKEADEWADELLNIVGLTKVKNNLATNMSGGQQQ
                     RTAIARALINKPKIILADEPTGNLDSETTENVYGILRDINKKYGTTFLIITHDQRIAE
                     KTDRIVQVKDGKINLDLRK"
     gene            complement(771..1928)
                     /locus_tag="PKK75_08160"
     CDS             complement(771..1928)
                     /locus_tag="PKK75_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ83612.1"
                     /translation="MKLAFDIALRFLKSGKGQTLLIALGISVGVAVQIFIGSLIQGLQ
                     ISLVDTTIGSSSHITVTAENDDKLIKNWERTVYEAKISSPEVLYVSPALDGAATLDYE
                     TDTSPVIIRGFDLEESDKIYKFKNNLYAGNLPDSKEQAIIGREMAVKSNTNVGDTITL
                     VTVRGNAYKLKVTGLFDLKVSSINDSWVVTDIETAEAILALEGVSSVEVQVDDVFLAD
                     EISRQVAYSLSGPDLKVDNWKDLNQQLLSGLNGQSVSSYMIQVFVLLSVLLGISSVLA
                     ISVVQRSKQIGILKAMGIQDKDASLIFVFQGLMLGALGALLGIALGMSLLLMFTKFAV
                     NPDGSPVVPIYISYGFITLSGLFAVFTATIASLIPARITSRLNPIEVIKNG"
     gene            complement(1912..2562)
                     /locus_tag="PKK75_08165"
     CDS             complement(1912..2562)
                     /locus_tag="PKK75_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNZ83613.1"
                     /translation="MDYKRAILEALKEEKKDLCIELSLESIEKNIYTIPELYENVLKT
                     GLYSIDYCSGDDCIWKEHIKTSIVRSVIECLYPYIIKLKKSAKPVNKKVVLACPEKEY
                     HEIGLRMVDDFFSILGYQSIFIGANTPKIQVLNAVTRTKADYLAVSVTDYYLLFEAQK
                     MIQLVRDVDKDIKVIVGGNAFKENMDSLKSIGGDTYIETFEDLIRLRDGEQDEISI"
     gene            complement(2571..>3014)
                     /locus_tag="PKK75_08170"
     CDS             complement(2571..>3014)
                     /locus_tag="PKK75_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83614.1"
                     /translation="EEAVLIDEGQKSIVTIDGLNTSYEGLVSFVSKVASTENDQSTKP
                     KVEVKIEITNPDENIAFGYEGEAQITIDSQDNVLVVKNESVKSENNEKFVYILNNNAA
                     VKTNVETGITDGYFTVIKSGIEENDTVIVNPPFDLRDGMMVKKAD"
BASE COUNT          824 a          640 c          477 g         1073 t
ORIGIN      
        1 tctcccttaa tcataataaa aagtattagt tatattatta taagccaata cttttttatt
       61 atgattattt tcttaaatcc aggttaattt tcccatcctt aacctgaact atcctgtcgg
      121 ttttttccgc aattctttgg tcatgagtta ttatcagaaa agttgtaccg tactttttat
      181 ttatatctct taatattcca tatacatttt ccgttgtttc agaatcaaga ttacctgtag
      241 gctcatccgc cagtattatt ttaggtttgt ttatcaaagc tcttgctatt gcagttcttt
      301 gctgctgacc ccccgacata ttggtcgcaa gattattttt aacctttgta agtcctacga
      361 tatttagaag ctcatcggcc cattcatctg cttccttggg aaccttgtag ttgtgaattt
      421 tgtatgggag gagcacattt tccttggcag taaattccgg aagaaggtaa tgaaactgga
      481 aaataaagcc tatggtcttg tttcttagga atgccagctc tgttttgtcc attgtatcgg
      541 ttcttttgcc gccgatataa acctctcctg aagttggctt atcaagggtt cctataatat
      601 ttagcatggt gctcttaccg cttcctgatt caccgataat ggaattaaag ctgttttctt
      661 caaatgaaat atttatatca aacaaaacct gtgtctttat tttctcgcca taaattttgt
      721 ttatattctt aagttctatt atattgttta attctttcat atcttcaccg ttaaccattt
      781 tttattacct ctataggatt tagtcttgat gttattcttg ccggaatcaa ggatgctatt
      841 gtagctgtaa aaacagcaaa caaaccggac aaggttataa atccgtagct tatataaatt
      901 ggcacaacag gactgccgtc aggatttact gcaaattttg taaacattaa caaaagactc
      961 ataccaagtg caattccaag caaggctcct aaagccccca gcataagccc ttgaaaaaca
     1021 aatattaagc ttgcatcctt gtcctgtatg cccattgcct taagaattcc tatttgtttt
     1081 gacctttgga cgacagatat ggcaagaacg cttgatatgc caagcagcac tgataaaagt
     1141 acaaatacct gtatcatgta gcttgaaaca ctctgaccgt ttaagccgct cagcaattgc
     1201 tgattcaaat ccttccagtt gtcgaccttc aagtcagggc ctgacaatga atatgccacc
     1261 tgcctggaaa tttcatcagc aagaaaaaca tcatcaacct gcacttccac tgagcttacg
     1321 ccttctaaag caaggatggc ttctgctgtc tctatgtctg ttacaaccca tgaatcattt
     1381 attgaagaca ccttcaggtc aaacaaacct gtaaccttta atttataggc gttgccccgg
     1441 acagtcacca gagttatggt atcccctaca tttgtattgg acttaacagc catttccctg
     1501 cctattattg cctgctcttt gctgtccgga agatttccgg catacagatt gttcttaaac
     1561 ttataaattt tatcgctttc ttccaggtcg aagcctctta ttataaccgg ggatgtgtct
     1621 gtttcatagt ctaaggttgc agccccgtcc aatgcaggtg aaacgtataa aacctccgga
     1681 ctgctgattt ttgcttcata aactgttctt tcccaatttt taataagctt atcatcattt
     1741 tctgctgtta cagtaatgtg ggaagagctg cctattgtag tgtccaccag gcttatttgc
     1801 aagccctgga ttaaagagcc tataaatatc tgaactgcta ctccaacgga aattcctaaa
     1861 gctataagca gtgtctggcc ttttcctgat tttaaaaatc taagagctat atcaaatgct
     1921 aatttcatct tgttcaccgt cccttagcct gattaaatct tcaaaagttt caatataagt
     1981 atccccgcct atgcttttta aggaatccat gttttctttg aaagcattgc ctcctacaat
     2041 aactttaata tccttgtcca catctcttac aagctgtatc attttttgcg cttcaaacaa
     2101 tagataataa tcagtcacac tgacagccag ataatcagcc tttgttcttg ttacagcgtt
     2161 taaaacttga atttttggag tgttagctcc tataaatatt gactggtatc ctaaaatact
     2221 aaaaaaatca tcaaccatcc taaggccgat ttcatgatat tccttttcag ggcatgccaa
     2281 aacaactttt ttgtttactg gctttgcaga ttttttaagc tttattatat agggatacag
     2341 gcattctata actgatctta caatggaggt cttgatatgt tccttccaga tacaatcatc
     2401 tccggagcag taatctattg aataaaggcc ggttttcaag acgttttcat acagttcagg
     2461 aatagtgtat atgttttttt caattgattc cagggacagc tctatgcata agtctttttt
     2521 ttcttctttc aaggcttcca gaatagccct tttataatcc atacttcctc ctaatcagcc
     2581 ttttttacca tcataccgtc tcttaaatca aaaggcgggt ttacaatgac tgtatcattt
     2641 tcttcgatgc ctgattttat cacagtaaaa taaccgtctg ttattcctgt ttcgacattt
     2701 gtttttacag cagcattgtt attcaatatg taaacaaact tttcgttgtt ttcacttttc
     2761 acactttcat ttttaacaac aaggacgtta tcctgtgagt cgattgtaat ttgtgcctcc
     2821 ccttcatagc caaaggcaat attttcgtcg ggatttgtta tttcaatttt tacttctacc
     2881 ttgggcttgg tagattggtc attttccgtt gaagcaactt ttgatacaaa gcttacaaga
     2941 ccttcatagg atgtgtttaa accatctatt gttacgatgc tcttctgacc ttcatcaatt
     3001 aataccgctt cctc
//
LOCUS       DAOPUT010000137         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_34152_length_2986_cov_20.922211, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000137 DAOPUT010000000
VERSION     DAOPUT010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34152_length_2986_cov_20.922211"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..2529)
                     /locus_tag="PKK75_08175"
     CDS             complement(28..2529)
                     /locus_tag="PKK75_08175"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HNZ83615.1"
                     /translation="MSKKILIIGGVAGGATAAARLRRLDEKSEIIMFEKGPYISYANC
                     GLPYHIGNVIKERASLLLQTPEAMSNKYNVDVRVESEVISINKENKTIDVKNLKTGEL
                     YTESYDTLIISTGSSPLKPPIEGINSPGIFTLWTVPDTDAIKKFIKEKNPKNAVVIGG
                     GFIGLEVAENLHHAGINVTVAEAMDQVMAPVDYEMAQLVQENMVSNNVELILGDGVSK
                     FRNEDDKVIITLASGKTLTADMVILSIGVRPNGQLAKDAGLELNQRGGIVVDKYLNTS
                     DPNIYAIGDVIEIEEYINKGKAMIALAGPANKQGRICANNIYGAREEYKGTQGTSIAK
                     VFDLTIASTGANEKTLVRMGMIEGKDYYTATISQKSHAGYYPESSEMILKLIFSTDGK
                     KIYGAQIVGEDGADKRIDTIATAIRLGGGIYDLKELELAYAPPYSSAKDPVNMLGFVA
                     ENIIEGRVKFSRWNAIDPSKQNEYTILDIREEAEHMVFEMPGTVNIPQGQLRKRINEL
                     NKDDNIIILCNIGVRSYNAARLLMENGFKNVSVYPAGTNFYKSMYYEYFNNHEREDID
                     DMAGTSRTDESNFDRPEEIKASIQVDCSGLQCPGPIMKVYETMKSANDGDIIQVSATD
                     SGFAKDVEAWSRRTGNTLLRIEKEGKSNIVYLKKGIGHSIIEKAQAKTEVKTSNDGKT
                     IIVFSGDMDKVLASFVIANGAAAMGRPVTMFFTFWGLNVLRKSEPQNIKKPFIDAMFG
                     KMMPQGVNKLKLSKMNMGGMGTAMMRKVMKDKNIDSLEDLIKKAMASGVKMIACTMSM
                     DVMGITKEELIDGVEFAGVGTYLGDAEESNVNLFI"
     gene            complement(2542..2841)
                     /locus_tag="PKK75_08180"
     CDS             complement(2542..2841)
                     /locus_tag="PKK75_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNZ83616.1"
                     /translation="MIDFNNKEETKKILSEKADLFKSIAHPVRLCILAMLIKEKQSNV
                     TDIQCCLDVPQPTVSQHLSKLKSAGILSAERNGTEIYYQIINEEVKKLIIQILNV"
BASE COUNT          798 a          634 c          452 g         1102 t
ORIGIN      
        1 tttttttcag ttatgagaaa actattatta gataaacaaa ttaacgttgg attcctcagc
       61 atcacctaaa taagttccaa ctccggcaaa ttcaacgccg tcgattaatt cttcctttgt
      121 aattcccatc acatccatgg acatggtgca ggcaatcatt tttactccac ttgccattgc
      181 ttttttaatc agatcttcca atgaatcgat atttttatcc ttcattacct tcctcatcat
      241 agctgttccc atgcctccca tattcatttt tgaaagttta agcttattga ctccctgagg
      301 catcattttg ccgaacattg catcaatgaa gggttttttt atattttgag gttcagactt
      361 tctcaaaaca ttaagtcccc agaatgtgaa aaacattgta acgggtcttc ccatggcagc
      421 agcaccgttt gcaattacaa atgatgccag aaccttatcc atatcaccgc tgaatacaat
      481 tatggtttta ccgtcattgg aagttttaac ttctgttttg gcctgagctt tttcaattat
      541 gctgtgtccg atacctttct taagatatac aatattgctt ttgccttcct tttctattct
      601 aagcagggta ttgcctgttc tcctgctcca ggcttccacg tccttggcaa atcctgagtc
      661 agttgcagac acttgaatga tatctccgtc atttgcgctt ttcattgttt catatacctt
      721 cataataggt cccgggcact gcaacccgct gcaatccact tgaattgatg cttttatttc
      781 ttctggcctg tcaaaattgc tttcatctgt cctgcttgtt ccagccatgt catcaatatc
      841 ctctctttca tgattgttga aatattcgta atacatagat ttataaaagt tagttcctgc
      901 agggtagact gaaacatttt taaacccgtt ttccatcaat aatctggctg cattgtatga
      961 ccggactccg atgttgcaaa gtataattat attatcatct ttattcaatt cattaattct
     1021 ttttcgaagc tggccttgag gaatatttac ggttccgggc atttcaaaca ccatgtgctc
     1081 tgcttcctct cttatgtcaa gaattgtata ttcattttgt tttgaaggat caatagcatt
     1141 ccatctgctg aatttaaccc ttccttcaat aatattttca gcaacgaaac ccaacatatt
     1201 aaccgggtct ttagctgaag aataaggcgg agcataagca agttccaatt cctttaaatc
     1261 ataaatgccg ccccccaaac gaatagctgt tgctattgta tctattcgtt tatcagctcc
     1321 atcttcacct acaatttggg ctccatagat ttttttgcca tcagttgaaa aaattagttt
     1381 cagtatcatt tcagaagact ccggataata ccctgcatgg gatttctgtg aaattgttgc
     1441 agtataatag tccttaccct caatcatacc cattcttact aaagtttttt cgttggcacc
     1501 tgtagaagca atagttaaat caaatacttt ggctattgat gttccctgcg tacctttata
     1561 ttcttcccgg gcaccataaa tattattggc acatattctt ccttgtttgt tggcagggcc
     1621 ggcaagagca atcattgcct tacctttgtt tatatattct tctatttcaa taacatcacc
     1681 aattgcatat atatttggat cagatgtgtt taaatattta tctacaacta taccgcctct
     1741 ttgattaagc tcaagccctg catcttttgc taactgaccg tttggtcgaa cgccaattga
     1801 caatataacc atgtcggctg ttaatgtctt tccgcttgca agagttataa taaccttatc
     1861 atcttcattt ctaaactttg aaacaccatc tcctaatatt agctctacat tgttagatac
     1921 catattttct tgcactaatt gagccatttc ataatctaca ggagccataa cctgatccat
     1981 tgcttcagct acagtaacat ttattcctgc gtggtgaaga ttttctgcta cttcaagacc
     2041 gataaatcct ccccctatta caactgcatt ttttggattt ttctctttaa taaatttttt
     2101 tattgcatca gtatcaggaa cagtccacaa agtgaaaatg cccggtgaat ttattccttc
     2161 aatgggcggt tttaaaggag atgaacctgt ggatataatt aatgtatcat aagattcagt
     2221 ataaagttct ccggttttta ggttttttac atctattgtt ttattttcct tgtttatgga
     2281 aataacttct gattccactc ttacatctac attatacttg tttgacatag cttccggtgt
     2341 ttgtaacaac agtgacgccc tttccttaat aacatttcct atatggtaag gaagtccgca
     2401 gtttgcatat gatatataag gtcccttttc aaacattata atttctgatt tttcatccag
     2461 ccttcttagt cttgctgcag cagttgcacc tcctgcaaca ccgcctatta ttaaaatttt
     2521 cttgctcata tttcctcccg attaaacatt taaaatttgt attattaatt tttttacttc
     2581 ttcgtttata atttgataat aaatttccgt tccgtttctt tcagctgaaa gtatgcctgc
     2641 tgatttcagt ttagaaagat gctgggatac agttggctgg ggaacatcaa ggcagcattg
     2701 aatatcagta acattgcttt gtttttcttt tataagcatt gccaatatgc acaatcttac
     2761 aggatgtgct attgatttga acaggtctgc tttttctgac aatatttttt ttgtttcttc
     2821 cttattgttg aagtctatca tttatcctcc cttaagtatc ggaagtaatt acatagcaat
     2881 tacctcacgc tgcttcaacg aggtgggtga ttaaaataca ctgcctagct tatttaaagg
     2941 tatacataac taaagaagca tgagatattt tcacctcgtc atatta
//
LOCUS       DAOPUT010000138         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_34672_length_2955_cov_17.744828, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000138 DAOPUT010000000
VERSION     DAOPUT010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34672_length_2955_cov_17.744828"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKK75_08185"
     CDS             complement(<1..543)
                     /locus_tag="PKK75_08185"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNZ83617.1"
                     /translation="MFMYTMEELKAHCLKCNKCRLASTRTNVVFGEGNVHADIMFVGE
                     GPGYFEDIQGRPFVGKAGQLLDKMIEAIKMKRSDVYIANVVKCRPPDNRNPLDDECDA
                     CLEYLRWQVKLINPKIIVCLGAVSAKKLIDSEFKISRQRGQFVKKGNFYFMPTYHPSY
                     LLRNEPSKKDAWEDFKNIAAK"
     gene            complement(621..1517)
                     /locus_tag="PKK75_08190"
     CDS             complement(621..1517)
                     /locus_tag="PKK75_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ83618.1"
                     /translation="MMQSHIGEIAALITAICWALNGIAFESAGKKIGSLSLSYIRLYI
                     AFVLLCITAYFMRGIALPLDADLNNWIWLSLSGVIGFVLGDIFLFQAYIEIGSRISLL
                     IMSAAPPLTALISFFILGERISALGLLGIFITMIGIGLVILSKNQVGNKVEFNRPVKG
                     LIYASIGALGQALGLIFSKKGMGTYDPLAATQIRIIAAALVLTLLITKNKKWPEILES
                     MKNKSAMPALVAGSIFGPFVGVAFSLLAVKYTATGIVSSISSISPILVIPASIYIFKE
                     KVTPKEILGAVVSIAGVILLFL"
     gene            1621..1896
                     /locus_tag="PKK75_08195"
     CDS             1621..1896
                     /locus_tag="PKK75_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ83619.1"
                     /translation="MLRIICGEISKLYRAIESIIKDIYPEAIPVPYLMSGGTDSRKYY
                     NVCNNIYRFAAVMMSKEDNESVHSVNEKITVENLKNMIRFFVEIMGR"
     gene            complement(2031..>2955)
                     /locus_tag="PKK75_08200"
     CDS             complement(2031..>2955)
                     /locus_tag="PKK75_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNZ83620.1"
                     /translation="VIIIVITGFYYADANVDRISPASIASGQEPDFTDVTIGKRSDEY
                     KAVFGEVVRAPARKGKNITNILLVGMDKSTGESSARSDSIIIVSVNKKSDSIKLISLM
                     RDMYIEIPGYDDNRINASYAFGGAKLLTETIYNSFMIPIDGFAEIEFSGFSKVIDTMG
                     GIDIELNKDESFYLNKEYRFNLVEGMNHLTGDQALAYSRIRYVGNADYERTERQRKVL
                     VTIYTKFKNSTIKTIYEMAKEILPLVSTDLSTMQLLDLTLTVMRMGTPDIETCRIPAE
                     GEFTPATINGMSVLLPDIAGNTELLKEYIGY"
BASE COUNT          912 a          566 c          521 g          956 t
ORIGIN      
        1 ctttgctgca atatttttaa aatcctccca ggcatccttc ttggagggtt catttcgcaa
       61 taaataagag ggatgatagg tgggcataaa ataaaagttt ccctttttaa caaactgccc
      121 cctctgcctg cttattttaa actccgagtc tataagtttt tttgcagaaa ccgccccaag
      181 gcagactatt atttttggat tgatcagttt aacctgccac ctcaaatatt ccaggcaggc
      241 gtcacattcg tcgtccaagg ggttcctgtt gtcaggaggc ctgcatttta caacatttgc
      301 tatatataca tcgcttcgtt tcatcttaat tgcctcaatc atcttgtcaa gaagctgacc
      361 tgccttcccc acaaagggcc tgccttgaat atcctcaaag tagcccggac cttctcccac
      421 aaacataata tcagcatgca cattaccttc accaaatact acattagtcc ttgttgaagc
      481 aaggcggcac ttattacatt ttaggcagtg tgcttttaac tcctccattg tgtacataaa
      541 gatacctcta agaattaatc tgttaaaatt atttttatta taatcaaatt tatgatttca
      601 tgtaatgtta ttcccgtctt ttacaaaaac aataatataa cccctgctat tgatacaact
      661 gccccgagga tttccttggg ggttactttt tctttaaata tatatatgga agcagggatg
      721 actaatatgg gagatattga tgaaatagat gaaacaattc ctgttgcagt atacttaaca
      781 gctaaaaggg aaaatgccac accgacaaaa ggtccgaaaa tagagccggc aaccaatgcg
      841 ggcatggcgc ttttgttttt cattgattcc aatatttcag gccacttttt atttttggtt
      901 attaataaag tcaatacaag agccgcagct atgattctta tttgtgttgc tgccaggggg
      961 tcataggtgc ccattccttt tttgctgaat ataagaccca gtgcctgacc taatgcacct
     1021 atgcttgcat aaatgaggcc ctttacgggt ctgttaaatt ccaccttgtt tccgacttga
     1081 tttttactaa ggataacaag gcctatgccg atcatagtga taaatatgcc aagtagtcct
     1141 aaagctgata ttctttcacc taaaataaaa aagcttatga gggctgtaag aggaggtgca
     1201 gcagacatta ttaataaaga tattcttgag cctatttcta tatatgcctg aaacaaaaaa
     1261 atatcgccaa gtacgaagcc tattactcct gataaggaca gccaaatcca gttgtttaaa
     1321 tcagcatcta atggaagtgc aattcctctc atgaagtatg cagttatgca gagtaataca
     1381 aaagcgatgt ataaccggat ataacttagt gataaggaac ctattttttt gccggcagat
     1441 tcaaaagcta ttccgtttaa tgcccagcat atagcagtaa tcagtgctgc tatttcacca
     1501 atatgtgact gcatgataat ctccttcttg tccaataaac tctaatggat agtatagaag
     1561 gtttttatta tatttgcaac aagaaaagag tgatgatata atttaaatat taattggtaa
     1621 atgctgagaa taatttgtgg tgaaatatca aaattataca gggcgataga gagtattatc
     1681 aaggatattt atccggaagc aatcccggtg ccctatctaa tgtcaggggg aaccgattca
     1741 agaaaatatt ataatgtatg taataatatt tacaggttcg ctgctgtaat gatgtcaaag
     1801 gaggataatg aatcagtaca ttcggttaat gaaaagataa ctgtagagaa tttgaagaat
     1861 atgatcaggt tttttgtgga gataatgggg aggtgaaata agattacttt tgttgtttct
     1921 taactagaac gaacgggcat gagaacagca gccattgcaa tcactgcatg ggaataaatt
     1981 tgctttatgg gtacaacacc gccgtgtact acggggtgtc tgtcaccgtt ttagtaccct
     2041 atatattctt tcaataattc cgtattacct gcaatatcag gcaacagtac agacataccg
     2101 ttaattgttg cgggagtaaa ctctccctcg gcaggaatgc ggcaggtttc gatatccggt
     2161 gttcccatac gcataaccgt taaggttaaa tctaatagct gcatagttga aagatcggtt
     2221 gaaacaagag gcaatatttc ttttgccatt tcatatattg tttttatggt tgaattttta
     2281 aactttgtat atattgttac taaaactttt ctttgtctct cggttctctc ataatcggca
     2341 ttaccaacat atcttattct ggaataagcc aaggcttggt caccagtcaa atgattcatg
     2401 ccttcaacaa ggttaaatct gtattcttta ttaagataaa aagactcatc tttatttaat
     2461 tcaatatcaa ttcctcccat agtgtcaatt acttttgaaa aacctgaaaa ttcaatttca
     2521 gcaaagccgt caataggtat cataaaacta ttatagatgg tttcagtcaa aagctttgca
     2581 cctccaaaag cataggaggc attaatcctg ttgtcatcat aacctggaat ttcaatatac
     2641 atatctctca taagagatat tagttttatt gaatcagatt ttttatttac agaaacaatt
     2701 ataattgaat ccgatcttgc cgagctttct ccggtactct tatccattcc aacaagtaat
     2761 atatttgtta tatttttgcc cttcctggca ggcgctctta ctacttcacc aaatacagct
     2821 ttatactcat ccgacctttt gccaattgtt acatctgtaa aatccggttc ctgtccggat
     2881 gcaattgaag caggagagat tctatctaca ttggcatcag cataataaaa tcctgtaatt
     2941 acgattatta tgact
//
LOCUS       DAOPUT010000139         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_35037_length_2931_cov_19.069889, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000139 DAOPUT010000000
VERSION     DAOPUT010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35037_length_2931_cov_19.069889"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKK75_08205"
     CDS             <1..527
                     /locus_tag="PKK75_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ83621.1"
                     /translation="EEDIAFGPENLGVAPEEIRRRVDDALNTVGMYEYRKRPPHLLSG
                     GQKQRIAIAGILALNSDCIILDEPTAMLDPSGRREVLETLKKLNAQGKTILLITHYMD
                     EAVQADRVIVMDNGNIKLDGTPKEVFRNIDEIKKYGLDVPQVTELTQELIKEGLKLPP
                     DIIDVKELVDLLCQ"
     gene            518..1378
                     /locus_tag="PKK75_08210"
     CDS             518..1378
                     /locus_tag="PKK75_08210"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HNZ83622.1"
                     /translation="MSIKAENIKYVYSEGTPFRTLALDDVNATINQGEFVGIIGHTGS
                     GKSTLIQLFNGLEPATEGKVEVDGTLVGEDKKKLKMIRQKVGLVFQYPEYQLFEETVA
                     KDVAFGPLNLGLSQDEIDLRVKEALELVGFNYKKIKDASPFDLSGGQKRRVAIAGVLA
                     MKPDYLILDEPTAGLDPAGRNEIFGQIKTLHDKSNLTVVLVSHSMEDIAKLVDRVIVL
                     SKGKVHMEGSPKEIYSQAEELEKIGLGIPQIASIIRELRKRGFNIRQDILTIEEAKTE
                     IIKEVRGRNV"
     gene            1371..2168
                     /locus_tag="PKK75_08215"
     CDS             1371..2168
                     /locus_tag="PKK75_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNZ83623.1"
                     /translation="MFRNLTIGQHYPVDSPIHDLDPRLKIIMTIIFIISLFLIESFTS
                     FLFVIVFLAIAITISRVPLKFVIRGLRPILFIILITFIINLLMTPGRVIFTLAFIKIT
                     EEGLRQAGFMAVRLTLLIMGTSLLTLTTSPIILTDGIESLLNPFKRFGLPAHELAMMM
                     TIALRFIPTLMEETEKIMKAQKSRGADFESGNIMSRAKNLVPLLVPLFISAFRRADEL
                     AMAMEARCYRGGENRTRMRQMKISKGDYAAAFVFTVYLAAVIIFKIW"
     gene            2162..2905
                     /gene="truA"
                     /locus_tag="PKK75_08220"
     CDS             2162..2905
                     /gene="truA"
                     /locus_tag="PKK75_08220"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNZ83624.1"
                     /translation="MVRNIKLLIAYDGTAYHGWQRQLNKPTIQETIEDALSAVMKEKI
                     DLTGSGRTDSGVHALGQAANFKADTRIPEDKIKIALNSNLPRDIRILESIDVHPDFNA
                     RFDALDKTYMYQIYNDRVNNPFYARYSCFVPQILNMDKMEEGLKLIEGTHDFKAFMAS
                     GSNVKTTVRTVYNAKLLKEGNLLKIYIKGNGFLYNMVRIIAGTLIDTGKGLTDISCIK
                     KALEDKDRTVLGQTAPPEGLFLMDVNYKK"
BASE COUNT          982 a          509 c          654 g          786 t
ORIGIN      
        1 ttgaggaaga tattgctttc ggtcccgaaa atttaggcgt tgcacctgag gaaatcagga
       61 gacgggtaga tgatgcctta aataccgtgg gcatgtatga atacaggaaa agacccccac
      121 accttttatc cggggggcaa aaacaaagaa ttgccatagc aggcatactg gccttgaatt
      181 cagactgcat aattttggat gaaccaacag caatgcttga cccttcaggc agaagagaag
      241 ttttggaaac attaaaaaag ctgaatgcac agggcaaaac catactgctt attacccact
      301 acatggatga agctgttcaa gcagacaggg taattgttat ggataatggg aatattaaat
      361 tggacggcac cccaaaggaa gtattcagaa atattgatga aataaagaaa tacggcttgg
      421 atgtccccca ggtaacggag cttactcagg aacttataaa ggaagggctg aaactgcctc
      481 cggatataat agatgtaaag gaattggtgg atttattatg tcaataaaag cagaaaacat
      541 aaaatatgta tacagtgaag gtaccccctt tagaactttg gccttggacg atgtgaacgc
      601 tacaataaac caaggtgaat tcgtgggtat aataggtcac accgggtccg gcaaatcaac
      661 cttgatacag ctttttaacg gccttgaacc tgccaccgaa ggtaaggttg aagttgacgg
      721 cactttagtg ggggaagaca agaaaaaatt aaaaatgata cggcaaaagg taggtttggt
      781 atttcaatat cccgaatatc agctgtttga agaaacagtt gccaaagatg ttgccttcgg
      841 acctttgaac ttaggtcttt cccaggatga aatcgactta agagtaaagg aagcactgga
      901 actggttgga tttaactata agaaaataaa agatgcatcc ccctttgatt tatccggagg
      961 gcaaaaaaga agggttgcaa tcgcaggagt acttgcaatg aaacctgact atcttatact
     1021 ggatgagcca actgcggggc tggaccctgc aggaaggaat gaaatcttcg gccagataaa
     1081 gacactccat gacaaatcga atcttactgt tgtcctggtt tcacacagta tggaggatat
     1141 tgcaaaactt gtggacaggg ttattgtctt atccaaaggc aaggttcata tggagggcag
     1201 ccccaaggaa atctattccc aggcagagga attggaaaag ataggtcttg gaattcctca
     1261 gatagcttct attataaggg agcttaggaa acggggattc aatataaggc aggacatact
     1321 cacaattgaa gaagcaaaga cggaaataat taaggaagtg aggggaagaa atgtttagga
     1381 atttaacaat cgggcagcat tatcctgttg actctcccat acacgacctt gatccgagac
     1441 taaaaataat aatgacaatc atttttatca tatcattatt tttgattgaa agctttacca
     1501 gcttcttatt tgttattgta tttttagcta ttgccataac aatttcaagg gtacctttaa
     1561 aatttgtaat aagaggctta agacctatat tgtttatcat attgataact tttataatta
     1621 accttctgat gacaccggga agagttattt ttacacttgc attcattaag attaccgaag
     1681 aaggtttgag gcaggcagga ttcatggctg taaggcttac actcctcata atgggaacat
     1741 ccctattgac actgacaacc tctccgatta tattgacaga cggcattgaa agccttctga
     1801 atccctttaa aagatttggt cttcccgccc atgaactggc aatgatgatg acaattgctc
     1861 ttcgtttcat tcctactctc atggaagaaa cagaaaagat aatgaaggct caaaaatcga
     1921 ggggtgcaga ttttgaaagc ggtaatatta tgagcagggc aaagaatctt gttcccttgt
     1981 tggttcctct gttcatcagc gctttcaggc gtgccgatga attggcaatg gcaatggaag
     2041 caagatgcta ccggggagga gagaacagaa caagaatgag acaaatgaaa atttcaaagg
     2101 gtgattatgc tgcagcattc gttttcactg tttatttggc agcagttata atatttaaga
     2161 tatggtaaga aatattaaac ttttaattgc ctatgacggc actgcttatc acgggtggca
     2221 aaggcaatta aacaagccta ccattcaaga aaccatagaa gatgcgttaa gtgccgtaat
     2281 gaaggaaaaa atcgatttaa cgggctcagg caggactgac agcggtgtcc atgccttggg
     2341 acaggcggcg aattttaaag ccgatacaag aattcctgaa gataagataa aaattgcact
     2401 gaattccaat ctgccccgtg acataaggat tttggaatct attgatgttc atccggattt
     2461 taatgcaaga ttcgatgctc ttgataaaac atatatgtat cagatttaca acgaccgggt
     2521 caataatccc ttctatgcaa ggtattcatg ttttgtcccc cagatattga acatggataa
     2581 aatggaagaa gggcttaaac tgattgaagg aacccacgat tttaaggcat ttatggcttc
     2641 cggatccaat gtgaaaacaa cagtacggac ggtgtacaac gcaaaacttt taaaagaggg
     2701 aaatttatta aaaatttata tcaagggcaa tggatttttg tataatatgg ttagaataat
     2761 tgcaggaaca ttaattgaca caggcaaagg attgactgat atttcctgca taaaaaaggc
     2821 acttgaagat aaggacagaa cagtcttagg gcagacagca ccgcctgaag gattgttttt
     2881 aatggatgta aattataaaa aatagcttga caattagttt ttattcatat a
//
LOCUS       DAOPUT010000140         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_35509_length_2902_cov_20.657885, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000140 DAOPUT010000000
VERSION     DAOPUT010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35509_length_2902_cov_20.657885"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2384)
                     /locus_tag="PKK75_08225"
     CDS             complement(<1..2384)
                     /locus_tag="PKK75_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83625.1"
                     /translation="MNKVKYCRKCGNTINNSVKFCRHCGALQNNQSKSPSVRDSQPSK
                     KNIKGPDVIDNSIKQKQKEKYFSDNETKTAKKKRGIFGKIAALLLIIFIAVNIINFLS
                     NKPNEPGSILTTTGNNENKSISEYVLSDVKISDDDLNIPAQSGFVSPDEPEITIENTF
                     INFSSYNLEGTKEVKVKRLNQISDSEKGYTIQAYDFEIEGISEFNVPVYIEMPYYPVI
                     EDNLYEESTIFVQYYNENESKWEMIPSWINAEANTVAILTNHFSPFGVFQDDNLEDAK
                     KIAHLFQYYGKYKGPTTPVYTTGTNIQRYYQFVDEKTFQTYIDYKTVPVNDFATSFLN
                     VMNHGASAGEYSLYALSPEIYKDTISGFSKAGKLFVLSKIAYQWYNDVATQDIIMDNI
                     FDLTEIAVGTVGTLAAAPELTVIAGGVWLTGLTYDVGTTAYEYMSENEPRYLAYRLAS
                     ESGYIKYLPDEEICVYWKEIEDPSYSSYDKELAKSRNKSIKLSGEYGWAQAFNAIQKK
                     YKENPIEMTKALERLLESYLDVFWYHFGYAELTDLLKSVETGIIFKSTLYDEWEWPVE
                     SEIQEYKANYRQKMKEYLRPIMKAMAQKALLELNELTLKEVVKLWDVVNMEIDFQIVD
                     ENLKEGEFFPSSPLSDMTIKLSKLSDEAIADEWICRERRERSNSVFTCNIYAYIKAGC
                     PTNVEFYEKNTETGVQELVISKEFTLSNPTVISIDKRMEDINGIYTGKAIGGNYTTGE
                     EMDLYYKAIRIESNKEGILIGPCLENGDYDPDYMVQCVLNKETNQYEGKVRRESG"
     gene            complement(2420..>2902)
                     /locus_tag="PKK75_08230"
     CDS             complement(2420..>2902)
                     /locus_tag="PKK75_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83626.1"
                     /translation="LMFFLTLGDGIGEAFKLLLSMLGIGAVLMTVLMVAGYVMLSIAY
                     GGKYYVLFKMDKKGVNHIQLDKQHKKAQALGFLTALIGLSSGNISAAGAGLLGASRKS
                     LYTNFKKVSSVKFSPRMNTIYLNESLNKNQIYAEKEDYLFIKEFILENCPKNIKIIEK
                     "
BASE COUNT          813 a          558 c          433 g         1098 t
ORIGIN      
        1 ccggattctc ttctgacttt tccttcatat tgatttgttt ctttatttaa aacacattga
       61 accatataat caggatcata atctccgttt tccagacagg ggcctatgag aattccttct
      121 ttgtttgatt caattctgat tgctttgtag tagaggtcca tttcttctcc ggtagtatag
      181 ttgcccccta tggctttacc ggtataaatg ccatttatat cttccattct tttatcaata
      241 gagataacag tcggatttga tagagtgaat tccttggaaa ttactagctc ctggacgcct
      301 gtttctgtat ttttctcata aaattctaca tttgtcggac atcccgcttt aatataagca
      361 tatatattac aggtgaaaac tgaattgctt ctttctcttc tttccctgca tatccattcg
      421 tcagctattg cttcatcact tagttttgat aatttaattg tcatgtcaga caatggagaa
      481 gatggaaaaa attctccttc ttttaaattt tcatccacaa tttgaaaatc aatttccata
      541 tttacaacat cccaaagttt tactacctct tttaaggtaa gctcatttaa ttccaaaagt
      601 gctttttgag ccatggcttt catgatcggt cttaaatatt ctttcatttt ttgcctgtaa
      661 tttgctttat actcttggat ttcagattct accggccatt cccattcatc atataaagtc
      721 gacttgaaaa taatgcctgt ttcaacagac ttaagaaggt cagtaagctc agcgtagcca
      781 aagtggtacc agaaaacatc taaataggac tccaagagtc tttctaatgc ttttgtcatt
      841 tctatgggat tttctttata ttttttttgt atggcattaa aagcttgtgc ccacccgtat
      901 tctccactca gcttaatgct tttgtttctg ctttttgcca gttctttatc atagcttgaa
      961 taagagggat cttctatttc tttccaatag acgcatattt cttcatccgg caaatattta
     1021 atgtaaccgg attcacttgc taaacggtag gctaaatatc ttggttcatt ctcagacata
     1081 tattcataag ctgtcgttcc aacatcataa gtcaaacctg taagccagac tcctcctgca
     1141 attactgtca gttcaggtgc tgctgcaagg gttccaactg tgcctacagc tatttctgta
     1201 aggtcaaaga tattatccat tataatatcc tgagttgcta catcgttgta ccattgatat
     1261 gcaatttttg aaagtacaaa aagtttccct gctttgctga aaccactaat agtatctttg
     1321 taaatttcag gagataaagc atataaagaa tactctcccg ctgatgctcc atgattcata
     1381 acatttaaaa aacttgttgc aaagtcattt accggtactg ttttataatc aatataggtt
     1441 tgaaatgttt tttcatcaac aaattgatag tatctttgga tatttgttcc ggtggtatat
     1501 acaggtgtcg taggtccttt atactttcca taatattgaa ataagtgggc aatcttttta
     1561 gcatcttcca aattatcatc ctgaaatacg ccaaagggtg aaaaatgatt ggttagaatt
     1621 gctacagtgt tggcttcggc atttatccag gaaggtatca tctcccattt gctttcgttt
     1681 tcattgtaat actggacaaa tatggtactt tcttcataaa gattgtcttc aattaccgga
     1741 tagtatggca tttcaatata gacaggcacg ttaaattcag aaataccttc tatttcaaaa
     1801 tcataggcct ggattgtata gcctttttca ctgtcactga tttgatttaa tctttttact
     1861 tttacttcct ttgttccttc caaattataa gaactaaaat tgataaaggt gttttcaata
     1921 gttatttccg gctcatcagg gctcacgaat cctgactgtg cagggatatt taaatcatca
     1981 tcacttattt ttacatcact taatacatat tcacttatgc ttttgttttc attatttcca
     2041 gtggtggtta aaatactgcc tggttcatta ggcttatttg ataagaagtt tattatatta
     2101 actgctataa aaattatcag caaaagtgcg gctattttcc caaatattcc tcttttcttt
     2161 ttggctgttt ttgtttcatt atcactgaag tatttttctt tttgcttttg cttaatgcta
     2221 ttatcaataa catccggtcc ttttatattt ttcttcgaag gttgtgaatc tctgacagat
     2281 ggtgattttg actgattgtt ctgcaaggca ccgcaatgtc tgcagaattt aacgctatta
     2341 ttgattgtat tgccacattt cctgcaatat tttactttat tcaatgcaac tcccccttat
     2401 atttatattt tgagacttgc tatttttcaa taattttaat atttttcgga caattctcta
     2461 aaataaattc ctttataaag agataatctt ctttttccgc ataaatctgg tttttattta
     2521 aggattcatt taaataaatt gtattcatcc tcggactgaa tttaacggag ctaacctttt
     2581 taaaatttgt gtataagctt tttctggatg ctcccaaaag gcctgctcct gctgcagaaa
     2641 tattccctga agaaaggcct attaaagccg taagaaatcc caaggcctgt gcttttttat
     2701 gttgtttgtc caattgtata tgattaacac ccttcttatc cattttaaac aatacatagt
     2761 atttaccgcc atatgctatg ctgagcatta catatcccgc aaccataaga acagtcatca
     2821 atacggcacc tattccaagc atggaaagta aaagcttaaa ggcttcccct ataccatctc
     2881 ctaatgtcaa aaagaacatt aa
//
LOCUS       DAOPUT010000141         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_39031_length_2713_cov_18.583145, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000141 DAOPUT010000000
VERSION     DAOPUT010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39031_length_2713_cov_18.583145"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /gene="hprK"
                     /locus_tag="PKK75_08235"
     CDS             complement(<1..808)
                     /gene="hprK"
                     /locus_tag="PKK75_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNZ83627.1"
                     /translation="MQYVKLEDLVSYTGIEPVYYPEDVQSRIYTPDIVRPGLQMAGYF
                     EWFSFERVQIMGKAEILYLKTLDEEVKKDRLDKFFSFPIPALIVANEMEVEDAIINYA
                     KKYKRPVYKSKERTDKLVNIMINFLEEELAEETTLHGVCLEVFGVGVLLRGKSSIGKS
                     ETALELINRGHRLVADDAVIIKRVEGGLKATCPELTRHLMEIRGIGILDIQHLFGVGS
                     IRIEQFIELIVDLEEWDENKEYDRIGKDDEYTEILGLKVSTVIIPVRPGRN"
     gene            complement(823..2646)
                     /gene="uvrC"
                     /locus_tag="PKK75_08240"
     CDS             complement(823..2646)
                     /gene="uvrC"
                     /locus_tag="PKK75_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNZ83628.1"
                     /translation="MFNIQEELKALPHSPGVYQMKNASGEIIYVGKAKSLNKRVKQYF
                     QSRNHPPKIQAMIKNISEFEYIITDNEVEALILEANYIKKYRPKYNTLLRDDKQYPYI
                     KISVQEKYPRIYKVREVKKDGAKYFGPYPSAYAVNDIIDIIGNLYKLRKCSLKLDGAK
                     RCQRPCIYYHINRCTAPCSGNVDISKYREEVSKASAFLSGGEDIIKSLNDRMAKASQN
                     LEYEEAARYRDQIKALEVISEKQKAVSQESIDQDVIGSAIGSEEACIQIFFIRNGKII
                     GSEHYLLTNTENESREQIISSFLTQFYTGTVYVPKEIYIESHIEDMELLEKLLSQKRG
                     NKVSLKVPVKGEKSMLIKMTKKNALEILEKGSQRIRKNIDESMKAIEELKNILRLDGV
                     PERIEAYDISNIQGVQSVGSMVVFEKGMPSKSNYRRFKIKTVDGPNDYKSMEEVLERR
                     INRGLGEDGQGFGKMPDLILVDGGKGQTSIAEEVISNFGLDIPVCGMIKDDRHTTRGL
                     IYKNSEIELNKSHIVYQLIYRIQEEAHRFALDYHRNLRDKTLFSSELDNIKNIGEKRK
                     INLLKEFGSVDIIKTKSVDDLSSVPGMNIKAAESVYNYFHG"
BASE COUNT          687 a          612 c          383 g         1031 t
ORIGIN      
        1 tgtttctccc cggtctgacc ggtattataa cagtagatac cttaagtcct aaaatttcag
       61 tatactcatc atctttgccg attctgtcat attccttgtt ttcatcccac tcttccaaat
      121 caacaattaa ttcgataaac tgctctatcc ttatggagcc tacaccgaac aagtgctgta
      181 tatcaagtat tcctataccc cttatttcca tcaaatgcct tgttaattca ggacaagttg
      241 cttttagtcc tccttcaacc ctttttataa taacagcgtc atctgcaaca agcctgtgtc
      301 ccctgtttat tagttccaat gctgtttcgc ttttaccgat actgctttta ccccttaata
      361 atactcctac tccgaaaacc tccagacaaa caccgtgcag tgttgtttct tcagccagtt
      421 cctcttcaag gaagttaatc attatgttaa ctaatttgtc cgttctctcc ttagatttat
      481 atactggcct tttgtatttt ttcgcataat taataatagc gtcttcaact tccatttcat
      541 ttgccacaat taaagcgggt atagggaagc tgaaaaattt atccagccta tcctttttca
      601 cttcttcgtc caaagttttc aaatataata tttctgcttt tcccattatt tgaacccgct
      661 caaaggaaaa ccattcaaaa tatcccgcca tttgaagacc aggccttact atatcaggtg
      721 tataaattct ggattgaaca tcctcaggat aatacaccgg ttctattccc gtataagaaa
      781 ctaaatcttc aagttttaca tactgcatgt ctttaccatc ctctatccat gaaagtaatt
      841 gtatactgat tctgctgctt ttatattcat tcccggtacc gacgaaagat catccacgct
      901 tttggttttt ataatatcaa cagaaccgaa ttccttaagt aaatttattt ttcttttttc
      961 accaatattc tttatattgt caagttctga gctgaaaagt gtcttgtccc ttaaatttct
     1021 gtgatagtca agggcaaatc tgtgtgcttc ctcttgtatc ctgtaaatca gctgatagac
     1081 tatatggctt ttgttaagct caatttcact gtttttatat atcaatcctc tggttgtatg
     1141 cctgtcatcc ttaatcatcc cgcagacagg tatatctaac ccaaaattac ttataacttc
     1201 ctctgcaata cttgtctgtc cctttccccc gtcaaccaaa attaaatcag gcatcttgcc
     1261 gaatccctgc ccgtcttccc caagtcctct gtttattctt ctttccagta cctcttccat
     1321 gcttttataa tcattcgggc cgtctactgt ctttatttta aaacgcctgt aattgctttt
     1381 gctcggcatt cccttttcaa aaacgaccat tgagccaacg gattgaacac cttgtatatt
     1441 tgaaatatcg taggcttcta ttctttccgg aactccatcc agtcttaaaa tatttttcag
     1501 ctcttcgatt gccttcatgc tttcgtcaat gtttttcctt attctctgac ttcccttttc
     1561 caatatttca agagcatttt tctttgtcat tttaatcagc atgctctttt cgcctttgac
     1621 cggaaccttc agacttacct tgtttcctct tttttgtgat aaaagtttct ctaaaagctc
     1681 catatcttca atatggcttt caatatatat ttctttaggc acatatactg tacctgtata
     1741 aaactgtgtt aaaaatgaac ttattatttg ttcccttgat tcattttccg tatttgtaag
     1801 caggtagtgc tcacttccta tgatcttgcc gtttcgtatg aagaatatct gtatgcatgc
     1861 ttcttcggag cctatggcac tgcctatcac atcctggtct atgctttctt gagatacagc
     1921 tttttgcttt tcggaaatta cttcaagagc tttaatctgg tccctgtacc tggcagcttc
     1981 ttcatactcc aaattttggg aagcctttgc cattctatca ttcaaggatt tgataatatc
     2041 ttcgccgcct gataaaaagg ccgaggcttt gcttacttcc tccctgtatt tacttatgtc
     2101 aacgtttcca gagcagggag ccgtgcacct gtttatatga tagtatatgc atggcctctg
     2161 acatctcttt gcaccgtcaa gttttaaaga gcattttcta agcttgtata agtttccaat
     2221 aatatctatt atatcattaa cggcataagc acttggatag ggtccgaaat attttgcccc
     2281 atcctttttt acttccctaa ccttataaat tctcgggtat ttttcctgaa cagatatttt
     2341 aatatatgga tattgcttgt catctcttaa tagggtgttg tatttaggcc tgtacttttt
     2401 tatataattt gcttccaaaa tcagtgcttc aacttcattg tccgtaatta tatattcaaa
     2461 ctcggaaata tttttaatca tagcctggat tttaggcgga tgatttcttg attgaaaata
     2521 ttgttttact cttttgttta agcttttagc cttgcctaca taaataattt ctccggaggc
     2581 atttttcatt tgataaacgc cggggctgtg gggcagggcc tttaattctt cctgtatatt
     2641 aaacatttta gctcctgata attactattc atattatacc acgttttcct gattattaac
     2701 ataaatatta aat
//
LOCUS       DAOPUT010000142         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_43766_length_2506_cov_23.491636, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000142 DAOPUT010000000
VERSION     DAOPUT010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43766_length_2506_cov_23.491636"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..656
                     /locus_tag="PKK75_08245"
     CDS             138..656
                     /locus_tag="PKK75_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNZ83629.1"
                     /translation="MDNYKEIIPEQVDKNIFHLINNEWMLITAKKDDKINTMTASWGG
                     FGIIWNKRVAGIVIRTNRYTKEFVDGSSKFSLCFFDKEYKKTLAYLGSVSGRDEDKIT
                     KSNLTVNYIEDVPYFEEAEMVIVCRKLYAQQMKPECFIVKEMDNEFYPKKDYHTLYIG
                     EIEKIYIKQESN"
     gene            complement(704..1360)
                     /locus_tag="PKK75_08250"
     CDS             complement(704..1360)
                     /locus_tag="PKK75_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HNZ83630.1"
                     /translation="MTGLALKIIALSTMIIDHYGAIFQSNIMIYRIIGRLAFPIYCFL
                     LVEGYTHTSDIKKYASRLLIFAFISEIPFDLAFFGQVGTWHQNIFFTLFIGLAAIYFI
                     DKKYGKYNYRKSILITLAAAVLATLLRTDYQYIGIIYIVAFYYTRNYPKEKRLVAAAV
                     IMFVANIEAGWMQQFSLLALIAIYFYNSKPGLKNKILQMSFYIAYPLHLLIFYLINTQ
                     "
     gene            complement(1573..1881)
                     /gene="trxA"
                     /locus_tag="PKK75_08255"
     CDS             complement(1573..1881)
                     /gene="trxA"
                     /locus_tag="PKK75_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNZ83631.1"
                     /translation="MNTVKITKDNFEQEVLKSNKPVLLDFWASWCGPCKMISPIVDEI
                     AGEVTHTKVGKVNIDEQPELASAFGIMSIPTLVSIKDGKIVNKMVGVRPKASIMQMIA
                     "
     gene            2219..>2506
                     /locus_tag="PKK75_08260"
     CDS             2219..>2506
                     /locus_tag="PKK75_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83632.1"
                     /translation="MKELFLKTENGLIPLDEKMIEKYSLAEGRKSPFTRYYVVNKTGK
                     YKFDSEKETSSPGKDQMPAGEGAEDDEIVEFSETGAILSQSEILDFSDGTDS"
BASE COUNT          853 a          372 c          430 g          851 t
ORIGIN      
        1 aaatattatg aattataatg gaatatatat gttttgttat gatataatta gaacggggtt
       61 gagatatacc cctaacaact atattgaaat tacatgttac tgtataatga ttttaagtaa
      121 aataaaggag aaattttatg gacaattata aagaaattat acctgagcag gtggacaaaa
      181 atatttttca cttgattaat aacgaatgga tgctgataac agctaagaaa gatgataaaa
      241 ttaataccat gaccgcatcc tggggcgggt tcggaattat ttggaataaa agagttgccg
      301 gcatagtaat aagaactaac aggtatacaa aagaatttgt tgacggttca tctaaatttt
      361 cattatgctt ttttgataag gaatataaga aaacattagc atatttgggc tctgtttcag
      421 gaagggatga ggataaaatc actaaaagca atttgactgt aaattatatt gaagacgttc
      481 cttattttga agaagctgag atggttattg tatgcaggaa gctttatgct cagcaaatga
      541 agcctgagtg ttttattgtt aaagaaatgg ataatgagtt ttatcctaaa aaggactatc
      601 acaccttgta cataggagaa attgaaaaaa tttacataaa acaagaaagc aattgaattg
      661 aataatactg cttaaaaaaa catcgttcta tgggcgatgt tttttattgg gtatttatca
      721 gataaaatat cagcagatgc agcgggtaag ctatataaaa actcatctgc agaattttgt
      781 ttttaagccc cggcttactg ttgtaaaagt atattgcaat taacgcaaga agagaaaatt
      841 gctgcatcca gccagcctca atatttgcaa caaacataat cacagctgcg gcgaccagtc
      901 ttttttcctt cggataattt cttgtataat aaaatgccac tatataaatg attcctatgt
      961 attgataatc tgttctcaga agggtagcca ggacagcagc tgcaagagtt atcaagatgc
     1021 tctttctgta gttgtatttt ccatactttt tatctataaa ataaattgca gccaagccaa
     1081 tgaataaagt aaaaaatata ttttgatgcc aggttcccac ttggccgaaa aatgccaaat
     1141 caaagggtat ttcagaaata aaggcaaata ttaacagccg tgatgcatat ttttttatat
     1201 cgctggtatg tgtatatcct tcaaccagca aaaaacaata aatcgggaat gccaatcttc
     1261 cgattattct gtaaatcatt atattgcttt gaaaaatcgc cccgtaatga tctattatca
     1321 ttgtggagag tgctataatt tttaatgcca gaccggtcat gatttctcca ttaatcttta
     1381 attgattata taagtacagg ctgctgaatc gccttgcttc acctgtaata actatacacc
     1441 gatattaatt atatcatttt tatctataaa ttcaataaaa aaggcagttg gtattctgcc
     1501 ttttttgagg ttcagtttgg gttttgtttg ggttatatat ctttcacttt ttcttggagg
     1561 agaaaagtgt tattatgcta tcatttgcat tattgaagct tttggtctta ctccgaccat
     1621 tttgtttaca atttttccgt cttttattga gactaatgta ggtatactca tgattccaaa
     1681 tgccgatgct aattccggct gctcatctat attaactttg ccaactttag tatgagttac
     1741 ttcgcctgct atttcatcta ctattggaga aatcattttg cagggaccgc accaggatgc
     1801 ccagaagtct aaaagtacag gtttatttga ttttaaaact tcttgctcga agttgtcttt
     1861 tgttattttt acagtattca tattaataat ctccttttat tctttattat gatattattt
     1921 tacctgtttt caatactatt gctgtgacca ggtcacaaaa gattccataa tcaactattt
     1981 atttattacc atgaaatgat tttatcatgt ttaaaaacta ttacagtaat caagtcacaa
     2041 aatattccaa aatgaattat ttgttttctt actatagtat gattttacca tgtttaaaag
     2101 ctattacagt aatttagtca cgaaggtgtt cttttagaat taaactattt aaggtctaaa
     2161 attataatat gattctattt gaatttgggc atactataaa aaaataccgg agagcaaaat
     2221 gaaagaatta tttttaaaga cagaaaatgg gttaattccc cttgatgaaa aaatgataga
     2281 aaagtacagt ttagcagaag gcagaaaatc tccttttacc cgctattatg tagttaataa
     2341 aacagggaag tataaatttg attctgaaaa agaaacttca agtcccggaa aagaccaaat
     2401 gcctgcagga gaaggggctg aggatgatga aatagtagaa ttctccgaaa cgggagcaat
     2461 tctctcccag tctgaaatcc tggatttttc tgatggaacg gattca
//
LOCUS       DAOPUT010000143         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_46861_length_2392_cov_17.632863, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000143 DAOPUT010000000
VERSION     DAOPUT010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46861_length_2392_cov_17.632863"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..845)
                     /locus_tag="PKK75_08265"
     CDS             complement(111..845)
                     /locus_tag="PKK75_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HNZ83633.1"
                     /translation="MDYLYDGTFEGLMTCIYYHYKKQKAAGIYELSGYQQSLAYQSEI
                     VETDISKAKTVSDAINKLISREAYIHVYYCYLSNDANKENLILEFLIFAFKYGRNTMN
                     FYTHEKVLPINEIYKRVAREEHRVLGILRFSDIGGILYAKYSPDNDISILLADHFADR
                     YKFEKFIIFDEKRKKAVVYADNSWEIKENIMIDNFEFSLNEEMIHKLWKQYFTDLAIK
                     ERENINLQFQFVPARYRKNMTEFKMP"
     gene            complement(853..2100)
                     /locus_tag="PKK75_08270"
     CDS             complement(853..2100)
                     /locus_tag="PKK75_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HNZ83634.1"
                     /translation="MDDNLFEKLRILSDAAKYDVSCVSSGVERKNNTVGGIGNASAAG
                     ICHAWSSDGRCISLLKILLTNDCIYDCKYCLNRVTNPIERAAFTPEEVAELTIQFYRR
                     NYIEGLFLSSAVEKSPDHTMENIYRVLELLRYKYNFWGYVHVKVIPGANPLLVQKTGM
                     LADRMSVNIELPTRESLHILAPQKRIENILQPMSLIHNEISRAIEDRSHFKNSQRFVP
                     GGQSTQMIIGATPDSDKTILNLSQNLYDKFRMKRVFFSAYIPVMSSPNLPAVSSMPPL
                     LREHRLYQADWLMRFYYFKSAELFTEIEPNLDLEFDPKMIYALRNIDRFPIEINKASY
                     EDLLRIPGLGVTSAQRIVRERKNAELSYDSLKKMRTVLKRAKYFITVKGKYYGNTKFE
                     PEAIKAEIRMKEIQKQINIFELL"
BASE COUNT          695 a          479 c          393 g          825 t
ORIGIN      
        1 ttttacgcag tttcgcacac aaatttattt gttcgtaaac tcacataaat ttggtactca
       61 ttgcgagtga cctacctcta tttttcagaa aaaacatctt aaaaatataa ttagggcatc
      121 ttgaattcag tcatgttttt tctgtatctt gcaggcacga attgaaactg caaattgata
      181 ttttccctct ccttaattgc caagtcggta aaatattgtt tccacaattt atgtatcatt
      241 tcttcattta aagaaaactc aaaattatcg atcattatat tttctttaat ttcccagcta
      301 ttatcagcgt aaaccaccgc cttttttctt ttttcatcaa atatgataaa tttttcgaac
      361 ttatatctgt cagcaaaatg atcagccagt aaaatactta tgtcattatc aggagaatat
      421 ttagcataca aaattcctcc tatatcagaa aacctgagta tgccaagcac cctgtgctct
      481 tccctggcca ctcttttgta tatttcattg atgggaagaa ctttttcatg agtatagaag
      541 ttcattgtgt ttctgccgta tttaaacgca aaaatcaaaa attccagaat caggttttcc
      601 ttgttggcat catttgaaag atagcaataa taaacatgta tgtaagcttc tctggaaatc
      661 agtttattga tcgcatcact gacagtctta gcctttgata tgtctgtttc cacgatttca
      721 gactgatatg caagagactg ctgatagccg gacaattcat atatgcctgc agctttttgc
      781 ttcttatagt gataataaat gcatgtcatc aatccttcaa aggtcccgtc gtataaatag
      841 tccatttctc ctctataaca attcaaaaat gtttatttgc ttttgaattt ccttcattct
      901 tatttcagct tttattgctt caggttcaaa ctttgtattt ccatagtatt taccttttac
      961 agtgataaaa tattttgctc tcttaagaac cgtcctcatt tttttaagag aatcatagga
     1021 taattcagca ttctttcttt ctcttactat cctttgggcg ctggttaccc caagccccgg
     1081 tatccttaaa aggtcctcgt aggatgcctt gttgatttct atggggaacc tgtcaatatt
     1141 tctcaaggcg tatatcattt tcgggtcaaa ctccaaatca aggtttggct ctatttcagt
     1201 aaataattcc gcggatttaa aataataaaa gcgcattagc cagtctgcct gatataacct
     1261 atgttccctt aaaagaggtg gcatggagct taccgcaggc aggttgggcg atgacatcac
     1321 aggtatgtat gcagaaaaaa acaccctttt catcctgaat ttgtcgtaca aattctggga
     1381 caggttaaga attgttttat cgctgtcagg ggtggctccg attatcatct gtgttgactg
     1441 accgcccggg acaaatctct gggaattttt aaaatgactc ctatcttcaa tggcacggga
     1501 aatttcattg tgtatgagag acatgggctg aagtatgttt tcgattcttt tctggggtgc
     1561 cagtatgtga aggctttctc tcgtgggaag ctcaatattg acactcatcc tgtcagcaag
     1621 catgcctgtt ttttgaacca aaagaggatt tgctccaggg attaccttaa catggacata
     1681 gccccagaaa ttatacttgt atcttaaaag ctcaagaacc ctgtaaatat tctccattgt
     1741 gtggtcagga cttttttcca cggcagagct taaaaataat ccttcaatat aatttctcct
     1801 gtaaaactga atcgtgagct ccgccacctc ttcaggtgta aaggctgccc tttcgattgg
     1861 atttgtaacc ctgttcaaac aatatttgca gtcataaata cagtcgtttg taagaagaat
     1921 ttttaaaaga gaaatacatc tgccgtccga ggaccaggca tgacaaatcc ctgcagcaga
     1981 agcattccct attcccccaa ctgtgttgtt ttttcgttcc actccgcttg atacacatga
     2041 tacgtcatac tttgccgcat cagataaaat tcttagtttt tcaaacaaat tgtcgtccat
     2101 tattcccacc gtctttctga aagaatagtc tttaatggta aaataatcat ataacaaaaa
     2161 aagacaaatg tcaaacattt gttcgaacga atccgggtta tttttctgtg aaattctcat
     2221 taccataaaa atgggttgca tactatagat acatctccat tgtcgcaaca ccatttcgtt
     2281 ccattttact gttaaatgaa atgtgcaggc tttgaatttc tcaaaatttc aattaatgtg
     2341 tgaatatatt tttcaaaaaa ctgattttca agtcgattgt gctcgaaatt ta
//
LOCUS       DAOPUT010000144         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_47858_length_2357_cov_25.541268, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000144 DAOPUT010000000
VERSION     DAOPUT010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47858_length_2357_cov_25.541268"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..169
                     /locus_tag="PKK75_08275"
                     /pseudo
     CDS             83..169
                     /locus_tag="PKK75_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(225..2246)
                     /gene="fusA"
                     /locus_tag="PKK75_08280"
     CDS             complement(225..2246)
                     /gene="fusA"
                     /locus_tag="PKK75_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ83635.1"
                     /translation="MRTYNADKIRNVALLGHSSCGKTTITEALLLNTKVTNRMGKVED
                     GNTVSDFDKEEKERHVSIGTSVIPIEYQDTKINFLDTPGYFDFVGEVYGALKVADSAV
                     LVIDASSGIEVGTEKSWKYLEQRNMPRIIFINKLDKENIKFDDLLDSLRDKFGKKVIP
                     FALPIGKGENIDGYMDILKGQSYIDSKPVETFPDTKAKAEELKAILMESVAETDEELL
                     EKYFEGEEFTDEEIHTGLKKAVLAGELVPVIVGAAIKGLGADMLMNTVVEYLPNALDA
                     GSLKGNPSEPLSAFVFKTIVDPFVGKISLYKVMSGTMKKDTDVYNSDSSESERIGSVF
                     CLRGKEQIEVSEVSAGDIGATSKLQNFKTGDTISLKSAPIVYDRIDFPKPCYFMAITG
                     KTKDSDEKIGTGLQRLNEEDPTFSMERNIETKELIVGGQGNIQLDVIASKLKNNYKVD
                     VDLNAPKVAYRETIKGKSDVQGKHKKQSGGAGQYGDVHIRFEPTQEEFEFNEEIFGGS
                     VPRNYIPAVEKGLRECLDKGVLAGCKVVNIKATLYDGSYHDVDSNEMSFKIAASLAFK
                     KGMTEAKPILLEPIAKVEINIPDEYLGDIMGDMNKRRGRILGMEQQLDGSQIVRAEAP
                     MAEMYTYAIELKSMTQARGTFEVEFLRYDEMPSNLAEKVIADYKAENEN"
BASE COUNT          606 a          511 c          391 g          849 t
ORIGIN      
        1 atttagatcc aaacacaatt ctccaagtgg attacgacgg caggcagcta attattaatc
       61 actgaacatc agcgcaatta tcgaagcttt agttttaggt gtctatgcaa aacagggagt
      121 tgtcttgatc actccacaac agcctattaa caaggaggat aaattaggcc gacaaaaacc
      181 tcccggttac ataaccggga ggtttaaatc tagccaaaat ttatttaatt ctcattttcc
      241 gccttataat cagcaattac cttttctgcc aggttggaag gcatttcatc gtatcttagg
      301 aattcaactt cgaaggtgcc tcttgcctga gtcatggact taagctcgat tgcataggtg
      361 tacatttctg ccattggagc ttctgccctt acaatttggc ttccgtccaa ctgctgttcc
      421 ataccaagaa ttcttcctct tctcttgttc atgtcaccca ttatgtctcc caggtactca
      481 tcgggaatgt tgatttcaac ctttgctatt ggttcaagaa gaataggttt tgcctctgtc
      541 ataccttttt tgaaagctaa tgatgctgca attttaaatg acatttcgtt cgagtcaaca
      601 tcatggtagg aaccgtcgta caaggtagct ttaatattaa caaccttgca tcctgccaga
      661 acacctttat caaggcattc tctaagtcct ttttctactg ctggaatata gtttctcgga
      721 actgagccgc cgaatatttc ttcattaaat tcaaattctt cctgagttgg ttcgaatctg
      781 atatgaacat ctccatactg gcctgcaccg cctgattgtt ttttatgttt tccctgaaca
      841 tccgatttcc ctttaattgt ttctctgtag gcaacctttg gagcattaag gtcaacatca
      901 accttatagt tgttcttaag cttgcttgca attacatcca gctgaatatt gccctgtccg
      961 cctacaataa gctccttggt ttcaatgttt ctttccattg agaatgtcgg gtcttcttca
     1021 ttaagtctct ggaggccggt gcctattttt tcatcactgt cctttgtttt tcctgtaata
     1081 gccataaaat agcatggttt tggaaaatca attctatcat aaactatggg agcggatttt
     1141 aaggatatag tatcacctgt tttaaaattc tgaagcttgg atgtagcacc tatgtcgcct
     1201 gctgatactt ctgaaacttc tatttgctct tttcctctta agcaaaaaac gctgcctatt
     1261 ctttctgatt cgctgctgtc agaattgtaa acatctgtgt cttttttcat tgttcctgac
     1321 ataactttgt atagtgaaat tttccctaca aaggggtcaa caattgtttt aaatacaaat
     1381 gcagataatg gttctgaagg attgccctta agtgagcctg catccagagc attgggcagg
     1441 tattccacaa ctgtgttcat aagcatatca gcacccaatc cttttattgc agctcctacg
     1501 attacgggaa ccaattcacc ggcaagtaca gcttttttaa gtcccgtgtg aatttcttcg
     1561 tcggtaaatt cttctccctc aaagtatttt tccagtaatt cttcatctgt ttcagcgaca
     1621 gattccatta gaattgcttt taactcttct gccttagctt ttgtatccgg aaatgtttcc
     1681 accggttttg aatctatata gctttgtcct ttcaatatat ccatatatcc gtcaatgttc
     1741 tctcccttgc ctatgggaag tgcaaacgga attactttct taccgaattt atctcttaaa
     1801 ctgtcaagta agtcatcaaa tttaatattt tccttatcta atttattaat gaaaataatt
     1861 ctcggcatat ttctttgttc caggtatttc catgattttt ctgtccctac ttctattccg
     1921 gagctggcat cgataactaa tactgcactg tctgcaactt ttaaggcacc ataaacttcg
     1981 ccgacgaagt caaagtatcc cggtgtatct aaaaaattaa tttttgtatc ttggtattca
     2041 atcggtataa ctgatgttcc aattgataca tggcgttctt tctcttcttt atcaaaatcg
     2101 gatacagtat ttccgtcttc tactttaccc attctgtttg ttacttttgt atttaacaaa
     2161 agagcttccg ttattgtagt tttgccacag ctgctgtggc caagcagggc tacgtttctg
     2221 attttgtctg cgttatacgt tctcattaac tttattcctc ctatgggttg tgcttattta
     2281 ttctatttta cattaaaaat aaataataat caatataatt ttacgatacc cggcaaggta
     2341 tgtatgtaga catcccg
//
LOCUS       DAOPUT010000145         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_48045_length_2351_cov_17.571864, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000145 DAOPUT010000000
VERSION     DAOPUT010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48045_length_2351_cov_17.571864"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKK75_08285"
     CDS             complement(<1..626)
                     /locus_tag="PKK75_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNZ83636.1"
                     /translation="MEVKKICIVGTGTMAKGIVQTFAQAGYPVIVKGRPGKTFDTLEA
                     AIGKGLSKLVEKGRMEQSKMDEILGNITKTTTYEECADADLFIEAVAEDMVTKHEMFA
                     EIEAVAKEGAILSTNTSSLSITEIAAKSKAPGRIIGMHFFNPVPVMKLVEIIKGKLTD
                     EEVHAAIIDLSQKIGKTPVSVDEAPGFVVNRILIPMINEGIGILADGVA"
     gene            767..1609
                     /locus_tag="PKK75_08290"
     CDS             767..1609
                     /locus_tag="PKK75_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNZ83637.1"
                     /translation="MMIKKGLVFTTKLMNKSTLDNIFGLSAQLSYYLILSVFPFLIMS
                     ISLFCGYSDDIFNMLNSLSGVLPEDVHSIIHNVLKYSVNSCSKPYLTVSMLIILWSAT
                     SGSAAIINGINIAYGFNTKKSFLLLRLRGILFTLALMVSMQIVFYVIVAGRSLLLFVK
                     RMPVFHDYNFMLIDAVRFVLAFFVIFIILSAAYKFLPYEKVKFSYAYKGAVFAAAGSL
                     AGSYLYSRYVSTRVVYINSIYGNLSGLFIFIIWIYIMSIIFLMGAEVNYFAAKQKNGI
                     NGKS"
     gene            complement(1676..2284)
                     /locus_tag="PKK75_08295"
     CDS             complement(1676..2284)
                     /locus_tag="PKK75_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019782616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNZ83638.1"
                     /translation="MDQYFTKNPASEKEIFEFDWNVGKEVFNFYTSNSVFSKKGVDFG
                     SMLLIETIIRENEGFSGSILDLGCGYGPLGIIAARVFHNSTVTMCDINERALELSMMN
                     AKENKVEGRVRIISSSAFGNIKDNYDIIMTNPPIRAGKDVVFSFYEGSYEHLNQGGRL
                     YVVIQKKQGAPSTKEKLESLFGNCETADKRSGYFIFRSIKNN"
BASE COUNT          656 a          437 c          389 g          869 t
ORIGIN      
        1 gcaactccat ctgccaatat gccaatacct tcattaatca tgggtatcag tatcctgttt
       61 actacaaatc ccggcgcttc atcaacactt accggagttt ttcctatttt ttgtgacagg
      121 tcgataatgg cggcatgtac ttcttcgtca gtaagcttgc ctttgattat ttcaactaat
      181 ttcataacag gaaccggatt aaagaaatgc attcctatta ttctgcccgg tgcttttgat
      241 tttgctgcaa tttcagtaat tgacaatgat gatgtatttg ttgataatat tgcaccttct
      301 tttgcaacag cttcaatttc ggcaaacatt tcatgttttg tgaccatgtc ttctgcaact
      361 gcttcgatga ataaatcagc atctgcgcat tcttcgtatg tagtggtttt agtaatgttt
      421 cctaaaattt cgtccatctt ggactgttcc atcctgccct tttcaactaa ttttgaaagg
      481 cctttgccta ttgctgcttc taatgtatcg aatgttttgc cgggtctgcc tttaactatt
      541 acaggatatc ctgcctgggc aaatgtctgt acaattcctt ttgccattgt tcctgttcca
      601 actatgcaaa tttttttaac ttccatttca tcctccaaat aaattttttt tgcctggtca
      661 tattatatca tcattgttca aattaaatca agtgatttta agaatagctt gataatattt
      721 taacataata aatgcatgat atttgcaaat aataatgcag ggtgagatga tgattaaaaa
      781 aggcttggtt tttactacaa aacttatgaa taaatccacg ttggacaata ttttcggatt
      841 aagcgctcaa ttgtcatact atttaatttt aagtgttttt ccctttctta ttatgtcaat
      901 atccttattc tgcggctaca gcgatgatat cttcaatatg ctgaattctt tatcaggtgt
      961 actccctgaa gatgtgcact ctattattca caatgtttta aaatactcgg taaacagctg
     1021 cagcaagcct tacctgacag tcagcatgct cataatatta tggtcggcaa cttcgggaag
     1081 tgccgccatc ataaatggaa taaatatagc atatggattc aataccaaaa aaagcttcct
     1141 gcttttgagg ctgaggggaa ttttgtttac attggcatta atggtttcaa tgcagattgt
     1201 tttttatgtt attgtcgcag gaagatcact tttgcttttt gttaaaagaa tgcctgtttt
     1261 tcatgattat aattttatgc tgattgatgc ggtcaggttt gttcttgcat tttttgtgat
     1321 ttttattatt ttatcagcgg catataagtt tctgccatat gaaaaggtta aattttcata
     1381 cgcatataag ggtgcagtat ttgcagctgc aggttcatta gccggttctt acctttattc
     1441 ccgctatgtc agcaccaggg ttgtatatat caactctatt tatggaaact tgtcaggatt
     1501 atttatattt attatatgga tttacattat gtcgattatt tttctaatgg gtgctgaagt
     1561 aaattatttt gcagcaaaac aaaagaatgg aattaacggg aagagttaga aggaaaatag
     1621 tggacgtttg ataggagggt tcacccggat aattttcatt gcgtggaact atccgttagt
     1681 tgttttttat tgatctgaaa atgaaatatc cagatctttt atctgctgtt tcacaatttc
     1741 caaataagct ttcaagtttt tccttggtgc ttggagctcc ttgtttcttt tgaattacaa
     1801 cataaagtct gccgccttgg tttaaatgtt cataggaacc ttcataaaat gaaaacacca
     1861 catcttttcc tgctcttatc ggcgggttgg tcataataat atcataatta tcctttatat
     1921 tcccaaatgc agaagaagag ataatcctaa ccctgccttc aaccttgttt tcttttgcat
     1981 tcatcatgga caattccaat gccctttcat ttatatcaca cattgtaaca gtagaattat
     2041 gaaaaaccct ggcggcaata attcccaaag gaccatatcc gcagcctaaa tctaaaatag
     2101 agccgctgaa accttcattt tctcttataa tagtttctat taaaagcatg gaaccgaaat
     2161 ccacaccttt tttagagaat acactgttgc ttgtataaaa attgaaaact tcctttccta
     2221 cgttccagtc gaattcaaat atttcctttt cagatgcagg gtttttagta aaatattgat
     2281 ccatttttcc ttctccaaat agtcattatc tttattattc cagaggggac ggttctctct
     2341 ggacaccttt a
//
LOCUS       DAOPUT010000146         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_50940_length_2260_cov_20.703401, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000146 DAOPUT010000000
VERSION     DAOPUT010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50940_length_2260_cov_20.703401"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1764)
                     /gene="lysS"
                     /locus_tag="PKK75_08300"
     CDS             complement(271..1764)
                     /gene="lysS"
                     /locus_tag="PKK75_08300"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNZ83639.1"
                     /translation="MIRWCKKLDERQTGDLRQVRIDKLNDLINAGRDPYKISKFEVTS
                     NSKHIRDNFEEMEGKFVSIAGRIMSKRGQGKVGFYDIQDHLGRIQVFVKQDNIGEEEY
                     NWLKTYDIGDILGIKGEVFKTKTGEISVRAEEVFLLSKSLQTLPEKFHGLKDMDIRYR
                     QRYVDLIVNPEVKDVFLMRNKIIRGIREYLDGEGYLEVETPILSPIAGGANARPFITH
                     HNTLDIDMYMRIANELFLKRLVVGGLGDGVYEMGKMFRNEGMDANHNPEYTAMEVYKP
                     YADFNDMMTLTENIVAYVAKKARGTTKIEYQGKMIDFTPPWRRVKMQDMVKEFAGVDF
                     DLVNTDEEALKIAKERGIEITPLMTRGHVISELFEKYCEEHLEQPTFVTHHPVEISPL
                     AKRNVQDTRLTDRFEAFANTWEIANAFSELNDPIDQRERFEEQVRQKEAGDEEAHMMD
                     TDFVNAIEVGLPPTGGLGIGVDRVIMLITNQPTIRDIILFPTMKPID"
     gene            complement(1778..2248)
                     /gene="greA"
                     /locus_tag="PKK75_08305"
     CDS             complement(1778..2248)
                     /gene="greA"
                     /locus_tag="PKK75_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNZ83640.1"
                     /translation="MKNNETLITAEGLEELKAELDELKLVKRKEISEKIKAALAFGDI
                     SENAEYDEAKNEQANVESRIAKLEIMIKNAKVIKTSKKNGVVNIGSKVKIKDLEFDEE
                     MEYTIVGSAEADPFKGRISNVSPLGEALLGKKLGEIIEVESPVGDKIKYQIITV"
BASE COUNT          557 a          525 c          328 g          850 t
ORIGIN      
        1 ttgtcaactg tcaatttttt cttttttccc tgtccgcttt tttggacagg aatcatagtt
       61 taacacacag ggtttcctct gtcaactgcc attttctgta aggcttttgc tcccttgtct
      121 tttgtgacag cttttacagt ctaccacagc taaaatatgt tgtcaagcat ttttcaacaa
      181 aaaatcagca aaaaatagag ccaaaaaaac aagagcagca atgctgctcc ttcaaaattt
      241 cacggtcatt tccggtaatc cgggaattct ttaatctatc ggtttcatag tcgggaataa
      301 tatgatatcc cttattgtag gctgatttgt tattaacatt ataaccctgt caactccgat
      361 tcccagaccg cctgttggag gaagacctac ttcaattgca ttgacgaagt ccgtatccat
      421 catgtgagct tcttcatcac ctgcttcttt ttgtctaacc tgttcttcga atctttctct
      481 ttggtctata ggatcgttta attctgaaaa tgcgtttgct atctcccatg tatttgcaaa
      541 tgcttcaaac ctgtctgtaa gtctcgtgtc ttgaacattt ctcttagcca gaggtgatat
      601 ttcaacagga tgatgagtga caaatgtagg ctgctcaaga tgctcttcac agtatttttc
      661 aaacaactcg cttattacgt ggcccctggt cataagaggg gtgatttcaa ttcctctttc
      721 ctttgcaatt tttaaggctt cctcatctgt atttaccagg tcaaagtcaa ctcctgcaaa
      781 ttccttaacc atatcctgca tttttactct tctccaggga ggtgtaaagt ctatcatctt
      841 gccctggtac tctattttag tggttcctct tgctttcttc gcaacatatg ctactatatt
      901 ttcggttaag gtcatcatat cattgaagtc cgcatatggt ttataaactt ccatggcagt
      961 atactcaggg ttgtggtttg catccattcc ttcattcctg aacatctttc ccatttcata
     1021 aacgccatca ccgagacctc caaccacaag cctcttcaaa aacaactcgt ttgcaatacg
     1081 catatacatg tcaatatcca atgtattatg atgtgtgata aatggtcttg cattggctcc
     1141 acctgctatg gggctcaata tcggagtttc aacctctaag tatccttctc cgtccaaata
     1201 ctctctgatg cctcgtatta ttttattcct cattaagaaa acgtctttga cttcagggtt
     1261 aactattaaa tcaacatatc tttgtctgta tcttatatcc atgtccttta atccatggaa
     1321 cttttcagga agtgtctgca aggatttgga taaaagaaaa acttcctctg cccttactga
     1381 tatttcgcct gtctttgttt tgaatacctc gcctttaatg cctaaaatat cgccgatatc
     1441 gtaagttttc agccagttgt actcttcctc ccctatattg tcttgcttaa caaatacctg
     1501 aattcttcct aaatggtcct ggatatcata gaatccaacc ttgccctgtc ccctcttaga
     1561 cattatcctt ccggcaattg atacaaattt tccttccatc tcttcaaaat tatccctgat
     1621 atgttttgaa ttggaagtta cctcaaattt tgaaatcttg taagggtccc tgcctgcatt
     1681 tattaaatcg ttcaatttgt caattcttac ttgccttaaa tcgcctgttt gtctctcatc
     1741 caatttttta caccacctta tcatttgttc tttttattta aacggttatt atttgatatt
     1801 ttatcttatc tcccacggga gattcaacct caatgatttc accgagtttc tttcctaaaa
     1861 gcgcttcacc caagggtgaa acattagata ttcttccttt gaagggatct gcctcagcgg
     1921 agcctactat tgtatattcc atttcctcat caaattccaa atcctttatt ttaacttttg
     1981 aacctatgtt gacaactcca tttttcttgc ttgtttttat gacctttgca ttttttatca
     2041 taatctcaag ctttgcaatc ctgctttcaa catttgcctg ttcattttta gcttcgtcgt
     2101 attctgcatt ttcacttatg tctccaaagg ccagagcagc cttgattttt tcggatattt
     2161 cttttctttt tactaatttt agctcatcca attccgcttt taattcttcc aaaccttcag
     2221 ctgtaattaa agtctcatta tttttcatat tattgctcct
//
LOCUS       DAOPUT010000147         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_51078_length_2256_cov_15.433439, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000147 DAOPUT010000000
VERSION     DAOPUT010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51078_length_2256_cov_15.433439"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKK75_08310"
     CDS             <1..703
                     /locus_tag="PKK75_08310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNZ83641.1"
                     /translation="VENIDIELVRKKIKNLHLTVYPPNGRVRLSVPSHMDNEAVKSFV
                     LTKLPWIKKQKTKYTTQELLPAKEFISGEIHPFSDEIYLLNVVETRGKQYVELRNNKF
                     MDMYVRPGSTFEKREKLMKDFYRQKLKEIIPSYIAKWERIMGVRVDEFGIKLMKTRWG
                     TCNIRDKRIWINLELAKKAPRCLDYIIVHEMVHLLEKHHNKSFKEYMSRFLPDWKSIK
                     DELNGLNLDSGKRSY"
     gene            717..1133
                     /locus_tag="PKK75_08315"
     CDS             717..1133
                     /locus_tag="PKK75_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNZ83642.1"
                     /translation="MKSYRKVLTFNTKNRREFINITPQIEECLRESGIKEGLLLCNPM
                     HITASVFINDDESGLHRDFEAFLEKLAPEKPYSQYHHNGYEDNADAHLKRTIMGREVV
                     VAVTEGKLDFGTWEQIFYGEFDGKRAKRVLIKIIGE"
     gene            1222..1551
                     /locus_tag="PKK75_08320"
     CDS             1222..1551
                     /locus_tag="PKK75_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ83643.1"
                     /translation="MKKKILLLIFVIIISLILLTGCMPGDGKSTPSNPAGFFWGIWHG
                     WIAPFSLVMGLFYKNIRIYEVVNTGWWYDLGFYMAILGGFGSFSIFRKSSSKNSKVTR
                     RSNEKKR"
     gene            complement(1605..>2256)
                     /locus_tag="PKK75_08325"
     CDS             complement(1605..>2256)
                     /locus_tag="PKK75_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ83644.1"
                     /translation="DIIEEEPEVSGDMPKDVKRSLYFILASIFLWFTAYNALTTAFSR
                     YAARVWGMEGGSFASALMVAAAAAFLSYIPVGFISGMIGRKKTIIIGVITMTVSYFFI
                     HLFLKPSPMINVVFALTGFGWAAINVNSYPMVVEMSKSGDIGKYTGLYYTSSMAAQIF
                     TPIFSGFLLENVSYRTLFPYAVLFSTLSLFTMLFVKHGDIKPSKKKSAIENFNIEY"
BASE COUNT          821 a          374 c          459 g          602 t
ORIGIN      
        1 tgtagaaaac atagacatag aattagtaag aaagaaaata aaaaaccttc atcttacggt
       61 ttatcctcca aacggaagag taagattgtc agttcccagc catatggata atgaagctgt
      121 taaatcgttt gtgctgacaa aattaccctg gataaaaaag caaaagacga aatacaccac
      181 ccaggaactt ttgccggcaa aagagtttat atccggtgaa attcatccct tttcagacga
      241 aatatatctg ttaaatgttg ttgaaacaag agggaaacaa tatgtggaac taagaaacaa
      301 caaattcatg gacatgtatg taagacccgg cagcactttt gaaaaacggg aaaagctcat
      361 gaaagacttc tacagacaaa aattaaaaga aattattccc tcatatattg caaaatggga
      421 aaggattatg ggagtcaggg tggatgaatt tggcatcaaa ctgatgaaga ccaggtgggg
      481 aacatgcaat atccgggata aaaggatatg gataaacctt gagctggcaa agaaagcccc
      541 aaggtgcctt gattatatta ttgttcatga gatggtacat cttcttgaga aacatcacaa
      601 caaatcattc aaagaatata tgtcaagatt tcttcctgac tggaaaagta ttaaggatga
      661 attgaacgga ttgaatttag acagcggtaa gcggtcctat tagataagga gggcaaatga
      721 aatcttatag aaaagtatta acctttaaca caaaaaacag aagagaattt attaatatta
      781 caccgcaaat tgaagaatgt ttaagagaaa gcggcattaa agagggtttg ctgctctgca
      841 atcccatgca cattacggcc agtgtcttca taaatgatga tgaatccggg ctgcacaggg
      901 actttgaagc ttttctggaa aaacttgcac cggaaaagcc ctacagccag tatcatcaca
      961 acggatatga ggacaatgcc gatgcccact tgaagaggac aatcatggga agagaagttg
     1021 tagttgcggt aacagagggg aaactggact ttggcacctg ggagcagata ttctacggag
     1081 aatttgacgg gaaaagagcg aaaagagtat tgataaaaat tataggcgaa taaagtttac
     1141 ataataaaaa ttatgtaaac aattaatcaa cacaggaaat tttttttaat agaaatacta
     1201 atttaaaagg agacagaaaa aatgaaaaag aaaattttat tattaatttt tgttattatt
     1261 ataagtttaa ttctcttaac aggttgtatg cccggggacg gtaaatcaac accttcaaat
     1321 cctgcaggat tcttctgggg aatttggcat ggatggattg cacctttttc acttgtcatg
     1381 ggattgtttt ataaaaacat aagaatttat gaagttgtaa atacaggctg gtggtatgac
     1441 ctgggattct atatggcaat cctcggcgga ttcgggagct tttcaatttt tcgtaaaagc
     1501 agctcaaaga acagtaaagt aacaaggaga agcaatgaga agaaaagatg acagatatta
     1561 aaaagggaac aggttgatat aaagaagctg cctgacaggc agctttaata ttcaatatta
     1621 aaattttcta ttgcactttt tttcttagac ggttttatgt ctccatgctt tacaaatagc
     1681 atggtgaaca gggataatgt cgaaaacagc actgcataag ggaaaagagt tctatatgac
     1741 acattctcca gtaagaaacc ggaaaaaata ggggtaaata tctgggcagc cattgaagat
     1801 gtataatata atcctgtata tttgcctata tcaccgcttt tactcatttc aacaaccata
     1861 gggtaggagt tcacatttat ggcagcccat ccgaatcctg taagtgcaaa gactacgttt
     1921 atcattggac taggcttcag gaaaagatgt ataaagaaat atgaaacagt catagtaata
     1981 actccaatga taatagtctt ttttctgcct atcataccgg atataaaacc aacgggtata
     2041 taagacaaaa aagcagcagc agctgctacc atcaaggctg aagcaaagct tccgccttcc
     2101 attccccaaa cccttgcagc atatcttgaa aaagctgttg ttaaagcgtt ataggctgtg
     2161 aaccacagga aaattgacgc aagtataaaa tataggctcc ttttaacatc cttaggcata
     2221 tcgcctgata cttccggctc ttcttctata atatcc
//
LOCUS       DAOPUT010000148         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_58845_length_2052_cov_19.960941, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000148 DAOPUT010000000
VERSION     DAOPUT010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58845_length_2052_cov_19.960941"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKK75_08330"
     CDS             <1..489
                     /locus_tag="PKK75_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNZ83645.1"
                     /translation="MHDIEHTADLEGHEITKRLVKEFITPIEREDILLLLHKIDDITD
                     CIEDVLLHMYMYNVRVIKEDALKFSSLILSACEELIGAFKEFKNFKRSKEIRNKIIRV
                     NKLEEEGDNLYTESVRKLHLSPSDAIEIVTWTIAYNRFEKCSDACEEAANVIESVIMK
                     NI"
     gene            587..1231
                     /locus_tag="PKK75_08335"
     CDS             587..1231
                     /locus_tag="PKK75_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNZ83646.1"
                     /translation="MDKQNKISSAVIRRLPKYYRYLGELNKAGVNKTSSRELSEMTGF
                     SASQIRQDLNNFGGFGQQGFGYDVGNLHNEIGKILGLDKKYKIIIVGAGNIGQAIANY
                     TGFYEADYEVVALFDKNPKLVGLSIRNALIMDSDSIEEFLEKEDIDIAVICTPKSVSQ
                     QIAEQLVRCGIKAIWNFAPKDLKMPEEVYVENVHLNESLFSLTYYYNKMKKENK"
     gene            1388..>2052
                     /locus_tag="PKK75_08340"
     CDS             1388..>2052
                     /locus_tag="PKK75_08340"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNZ83647.1"
                     /translation="MAKENLNPLENSQMQVKTACDALGLDPAVYEILKEPQRVIEVNI
                     PVRMDDGSMKLFKGFRAMHCDAVGPTKGGVRFHPGVSLEEVKALSIWMTFKGGVMGLP
                     YGGGKGGVICDPLTLSQGELERISRGYIQKLYKYIGEKEDIPAPDAGTNGQIMAWMVD
                     EYNKLTGTSALGVITGKPVAWGGSEGRNEATGFGVAIVTREAAKKLGIDIKQSKVAIQ
                     GFG"
BASE COUNT          752 a          295 c          446 g          559 t
ORIGIN      
        1 atgcatgata tagagcatac agctgatctg gaaggtcatg aaataactaa aagacttgta
       61 aaggaattca ttacacctat tgagcgtgaa gatattctgc ttttgctgca taaaattgac
      121 gatattacag attgcatcga agatgtgctc cttcatatgt atatgtataa tgtcagagtt
      181 atcaaggaag atgcattaaa gttttccagc ttgattttat ctgcatgtga agaattaata
      241 ggtgcattca aggaatttaa aaactttaaa aggtcaaagg aaatccggaa taaaattata
      301 agagtcaaca aattggagga ggaaggcgac aacctttata ctgaaagtgt tagaaagctc
      361 catttatcac cttcggatgc catagaaata gtgacatgga ccatagccta caatcgcttt
      421 gaaaaatgca gcgatgcttg tgaagaagca gctaatgtta ttgaaagtgt cattatgaaa
      481 aacatataat tgaagattgt gaaataaatc tctatatttt ttaaaaacat gttgcaatgc
      541 tgctgaaaga ttgttaaaat atatcaatag gaggcacgaa gcacatatgg ataaacaaaa
      601 caaaatttca tcggcagtaa taagaagatt acctaaatat tacaggtact taggggaatt
      661 aaacaaagca ggagttaaca aaacttcctc aagagaatta agtgaaatga caggtttctc
      721 agcttcacag attagacagg atttgaataa ctttggaggg ttcggacagc agggattcgg
      781 ctatgatgtt ggaaatttgc acaacgaaat cggtaagatt ttaggtttgg acaaaaaata
      841 caaaattata atagtaggtg caggaaatat aggccaggca attgcaaatt atacaggctt
      901 ttatgaagct gattatgaag tggttgctct gttcgacaaa aatcccaagc tcgtaggatt
      961 atccatcagg aatgcgttga ttatggattc cgacagtata gaagaattcc ttgaaaaaga
     1021 agatatagac attgcagtta tctgtacccc gaagagtgta agccagcaga ttgcagagca
     1081 attggtgcgc tgcggcataa aagctatttg gaactttgct ccaaaggatt taaaaatgcc
     1141 agaagaggtg tacgtggaaa atgttcattt gaatgaaagt ttattctcac taacatatta
     1201 ttacaataag atgaaaaaag aaaacaaata gttacattaa aaacagaagc caaaagtctt
     1261 ctgtttttac tttttgtcct tgaaataatg ccggcagagt ggtagaatag gttgtcaggc
     1321 aatattgtta ttaatttagc aatatttaag ataaaatcta acatactaat attttggagg
     1381 tatagtaatg gcaaaagaga atttaaatcc attggaaaac tctcaaatgc aagtaaaaac
     1441 tgcttgtgac gctttaggat tagacccggc tgtgtatgaa attttaaaag aaccacagag
     1501 agtaattgaa gttaacatcc ctgtaagaat ggatgacgga agcatgaagt tattcaaagg
     1561 cttcagagca atgcactgtg atgcagtcgg ccctacaaaa ggcggagtac gattccatcc
     1621 aggtgtatca ttggaagaag taaaagctct ttctatctgg atgacattta aaggcggagt
     1681 tatgggtctt ccttatggcg gcggtaaagg tggagttata tgtgatccat tgacattatc
     1741 tcaaggagaa ttagaaagaa tatcaagagg ttatattcag aagttatata agtacattgg
     1801 agaaaaagaa gatataccgg cacctgatgc aggaaccaac ggacaaatta tggcctggat
     1861 ggtagatgaa tacaacaaat taaccggaac atcagcatta ggcgttataa caggaaaacc
     1921 cgttgcatgg ggcggctcag agggcagaaa tgaagcaaca ggatttggtg ttgcaatagt
     1981 tacaagagaa gctgccaaaa aacttggaat tgatattaaa caatctaaag ttgccattca
     2041 gggatttgga aa
//
LOCUS       DAOPUT010000149         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_59542_length_2037_cov_16.927346, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000149 DAOPUT010000000
VERSION     DAOPUT010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59542_length_2037_cov_16.927346"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /gene="pepT"
                     /locus_tag="PKK75_08345"
     CDS             complement(<1..1127)
                     /gene="pepT"
                     /locus_tag="PKK75_08345"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNZ83648.1"
                     /translation="MKVHERFLKYVKIDTQSLPEAENIPSSKKQKDLGRLLVEEMISI
                     GIKDAYMDENGYVYGTVKGNADGPAIGFIAHMDTSPDMPGNNVKPKIIYDYDGSDIVL
                     NEEKQIVMETKTFDHLLKYKGQDLIVTDGTTLLGADDKAGIAEIMSMAEYFINNPQIK
                     HGTIKIGFTPDEEVGRGADLFNIEEFNADYAYTVDGGEIGELEYENFNAASAKVKISG
                     VNIHPGTAKNKMKNSILIGMEFHNMLPVHETPENTEGYEGFSLLNDMSGNVEETLLEY
                     IIRDHNLEKFNIKKDRFQKITYYLNEKYGQGTVELELKDQYFNMKEKILPHMHIIENA
                     VRAMEDIGIEPSVVPIRGGTDGARLSYMGLPCPNLCTGGHN"
     gene            1409..>2037
                     /locus_tag="PKK75_08350"
     CDS             1409..>2037
                     /locus_tag="PKK75_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNZ83649.1"
                     /translation="MSFIKEGYKVKSATVIKNLEKRNMEGYYCETVEEAVEKAMSMIK
                     QDDIVGWGGSTTIDEIGIKKLLEEKNIAVYDRDKETDPKEKVKMMKKALTSDVFLTSA
                     NAITMDGELLNIDGNGNRLAAYCYGPDSVIVVAGMNKIVTDLDVALKKARLDATVPNT
                     FRTNSQTPCHFTGKCSDCTMSDTICGQILVTRFCRPKNKIKVILVGENLG"
BASE COUNT          583 a          392 c          384 g          678 t
ORIGIN      
        1 aagttatgac ctccggtaca taaattaggg catggcaatc ccatatagga caatcttgca
       61 ccatctgtgc cgcctcttat tggaaccacg cttggctcaa tacctatatc ttccattgcc
      121 cttacagcat tctcgataat atgcatatgg gggaggattt tttccttcat gttgaagtat
      181 tgatctttta attccaattc tactgttcct tgaccgtatt tttcattaag gtagtaagtg
      241 attttttgga atctatcttt ttttatattg aatttttcca aattgtggtc tcgtataatg
      301 tattctaaaa gcgtttcttc cacgttcccg gacatatcgt tcaagaggct gaaaccttcg
      361 tagccttctg tattctcagg ggtctcatgc acaggcagca tgttgtgaaa ttccattcca
      421 atgagtatgg agtttttcat tttattttta gcggttcccg gatgaatatt tactccgctg
      481 atttttacct ttgcacttgc tgcattgaag ttctcatact ccaactcacc gatttctccg
      541 ccgtcaacgg tataggcata atctgcattg aattcttcaa tattaaacag gtctgctccc
      601 cgtcccactt cctcgtcggg agtaaaacct atttttatgg taccgtgctt aatttgagga
      661 ttgtttataa agtattctgc cattgacatt atttctgcaa ttcccgcctt gtcatctgcc
      721 ccaagaaggg ttgtgccgtc tgttacaatc aagtcctgcc ctttgtattt caacaaatgg
      781 tcaaaggttt tcgtttccat tacaatttgt ttttcttcat tcaatactat gtcgctgccg
      841 tcgtaatcat atattatctt tggctttaca ttgttccccg gcatatccgg cgatgtgtcc
      901 atatgggcaa taaaccctat tgcaggccca tctgcattgc ctttgacagt accgtaaaca
      961 tatccgtttt catccatgta tgcatcttta attcctattg atatcatttc ttcaaccaga
     1021 agccttccca agtctttttg ttttttgctg cttggaatat tttctgcttc cggcagagat
     1081 tgagtatcaa tttttacata ttttaaaaac ctctcgtgta ctttcatctt gtcctctctg
     1141 tgcttattat ttagatacta tcagtatatc attgtggatt ttgtatatca atgtttatta
     1201 aatagcagca ggtaataaaa agaatctcgc agtctcgctg cacaatttta tttgctcctt
     1261 tcgtcgcata aaatcggcgc tcgctgtgaa tgacctgcca gccatttttt tgaaaaaaga
     1321 ctcaaaaaaa tggggttagg gcatctaatt gttgaattaa ggattagatt gatatataat
     1381 atggacaaga atagatagga ggtagtttat gagttttatt aaagaaggat acaaggtaaa
     1441 aagtgctact gttataaaga atcttgaaaa aaggaatatg gaaggctact attgcgaaac
     1501 tgttgaagaa gcagtagaaa aagccatgtc aatgattaaa caggatgaca tagtcgggtg
     1561 gggcggttca actaccattg atgaaattgg aatcaagaag ctgttagagg aaaaaaatat
     1621 agctgtttat gacagagata aagaaactga ccctaaagaa aaagttaaaa tgatgaaaaa
     1681 agcattaact tctgatgttt tccttacaag tgccaatgca attactatgg acggtgagct
     1741 tttaaacatt gacggaaacg gaaacaggct tgctgcatac tgctacggtc ctgacagtgt
     1801 tatagttgta gcaggaatga ataaaatagt aactgatttg gatgttgcac ttaaaaaggc
     1861 gagacttgat gcaactgttc caaacacgtt caggacaaat tcgcaaaccc cctgccactt
     1921 tacgggaaag tgttcagatt gcaccatgag cgacaccatc tgcggacaaa ttcttgttac
     1981 aaggttctgc agacctaaaa ataaaataaa agtaatttta gtcggtgaaa atttagg
//
LOCUS       DAOPUT010000150         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_76633_length_1730_cov_20.118806, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000150 DAOPUT010000000
VERSION     DAOPUT010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76633_length_1730_cov_20.118806"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1730
                     /locus_tag="PKK75_08355"
     CDS             <1..>1730
                     /locus_tag="PKK75_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNZ83650.1"
                     /translation="VDGTIIFGNSSIDESMLTGESMPVEKKAGDKVVGASINKSGFFK
                     FEAQRVGDDTTLAQIIKLVEEASSSKAPISKMADKISGIFVPVVIATALLATIAWMIS
                     GADFEFALSIGIAVLVISCPCALGLATPTAIMVGTGKGAENGILVKSAEALEIAHHVQ
                     TVVMDKTGTITEGKPRVTDLVSDGIDELEMLRIAASAEKGSEHPLADAIVEEAKQLNL
                     DLYDAENFEAIPGQGLKAYVNNKKYYLGNLRLIKEQKIDIGSFEEKSIKFADEGKTPL
                     YFADENKVLGIISVADIVKPTSQAAIEEFEAMGIEVVMLTGDNKKTAEAIRKQLGITR
                     AVAEVLPQDKEREIRKIQEQGRKVAMIGDGINDAPALARADVGIAIGAGTDVAIESAD
                     IVLIRNDLLDAVTAVQLSKATIKNIKQNLFWAFIYNTIGIPLAAGVFYGILGWKLNPM
                     FAGAAMSLSSVSVVSNALRLKFFKPKRHSKDTLVYYGNAAEDHEDKELEKEKKENKEN
                     TSAVKKDENNKNNENDENICIVENMEKIREKGDYEMKRILEVEGMTCGHCKKAVEKAL
                     KAVDGVDDALV"
BASE COUNT          610 a          287 c          424 g          409 t
ORIGIN      
        1 gttgacggca caatcatatt cggcaattca tcaatagacg aatcaatgct cacaggtgaa
       61 agcatgcctg ttgaaaagaa ggctggagac aaggtggtag gtgcaagtat caataaatcc
      121 ggtttcttta aatttgaagc tcaaagggtg ggagatgaca ctactttagc tcagattata
      181 aaattggttg aggaagccag ctcatcaaag gctcccatat caaagatggc tgataaaata
      241 agcggtatat ttgttcctgt agtaatcgca acagcccttt tggcaacaat agcatggatg
      301 atttcgggag cagatttcga gttcgcccta tccataggaa tagctgtcct ggttatttca
      361 tgtccatgtg cattaggact tgcaacgccc actgcaataa tggttggcac aggaaagggc
      421 gctgaaaacg gaattcttgt taaatcggct gaagcgctgg aaatagctca tcatgtgcag
      481 acggtggtaa tggataagac cggtacaata accgaaggca agccgagagt tacggacctt
      541 gtttctgatg gaattgatga attggaaatg ttaaggatag cagcttctgc agaaaagggc
      601 tcggagcatc ctcttgccga tgcaatagtc gaagaagcta aacagttaaa tttagacctg
      661 tatgatgcag aaaattttga agcaattccc ggccaagggt taaaagcgta tgttaacaat
      721 aaaaaatatt atcttggcaa cttacgctta ataaaggagc aaaaaataga tattggaagc
      781 tttgaagaaa aaagcataaa atttgcagat gaaggaaaaa cccccttata ttttgcagat
      841 gaaaacaagg tgcttggcat aatatcagtt gccgatattg ttaaacctac aagtcaggcg
      901 gcaattgaag agtttgaagc aatgggtatt gaggttgtaa tgcttacggg agataacaaa
      961 aaaactgccg aagcaataag aaaacagctg ggaattacac gggccgttgc ggaagtactg
     1021 ccccaggaca aggaaagaga aatcagaaaa attcaggaac aagggaggaa ggttgccatg
     1081 ataggcgacg gcattaatga tgcgccggcc cttgccaggg cagacgtagg cattgccata
     1141 ggagcaggta cggatgttgc catagaatca gcggacatcg tgcttatcag gaacgacctg
     1201 ctggatgccg tcactgcagt acagctaagc aaggcgacaa taaaaaatat aaaacagaat
     1261 ttattctggg catttattta caacaccatt ggtatacctt tggcggcagg agtattttac
     1321 ggaatattag gctggaagct caatcctatg tttgcaggag ccgcaatgag tttaagctcc
     1381 gtatcagtgg tttccaatgc attgagattg aagtttttta aaccgaaaag acacagcaaa
     1441 gacacccttg tatattatgg aaatgccgca gaagaccatg aagacaaaga attggaaaaa
     1501 gaaaaaaaag aaaataaaga gaatacttct gctgtaaaaa aagatgaaaa caataaaaac
     1561 aatgaaaacg atgaaaatat ttgtattgtt gagaatatgg aaaaaataag agaaaaagga
     1621 gattatgaaa tgaaaaggat actggaggtt gaaggaatga cctgcggaca ctgcaaaaaa
     1681 gccgttgaaa aagctcttaa agctgtggat ggtgtggatg atgctctagt
//
LOCUS       DAOPUT010000151         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1411
            NODE_80962_length_1671_cov_16.572401, whole genome shotgun
            sequence.
ACCESSION   DAOPUT010000151 DAOPUT010000000
VERSION     DAOPUT010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854616
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:59:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,656
            Genes (coding)                    :: 1,649
            CDSs (with protein)               :: 1,649
            Genes (RNA)                       :: 18
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80962_length_1671_cov_16.572401"
                     /isolate="AMR_MDS_1411"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..246)
                     /locus_tag="PKK75_08360"
     tRNA            complement(162..246)
                     /locus_tag="PKK75_08360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Leu,seq:cag)
     gene            complement(302..772)
                     /locus_tag="PKK75_08365"
     CDS             complement(302..772)
                     /locus_tag="PKK75_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNZ83651.1"
                     /translation="MINIKVPGRCEYRIENIVLDYNGTIAVNGKIIPSIAENLAILSN
                     MANVYVLTADTYGSASMECRALKLTVKTFPNDKAADYKAKIVEELGKDNTVCFGNGYN
                     DIRMFKAAALSIAVLENEGMCSKLLAESDIMVKSMEDGINLLLNPNALISTLRG"
     gene            complement(886..>1671)
                     /locus_tag="PKK75_08370"
     CDS             complement(886..>1671)
                     /locus_tag="PKK75_08370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ83652.1"
                     /translation="RKLWKNIPKKSGEKMENKVLAIVDGREIKESDLNILIKNLGQNA
                     AYFQGEEGRKKLIDELVMHELMYLDAVDKNLESEEEFKEVLNEMKRSMLQQYNMRKMF
                     NSITLSEDELKDFYEKNKSLYNRQEMVKASHILVDTLEKANEILEDLTDGLSFEEAAK
                     EYSTCPSKQSGGALGQFGKGQMVREFEDAVFSMRVGEISTPVKTQFGYHIIKLTEHTP
                     AKSADFEESYQELKENYFALKQENVYRNKKEELTKKYDVVIFE"
BASE COUNT          437 a          366 c          256 g          612 t
ORIGIN      
        1 ttaatcatag cattagctaa gtgtcaaaac ctgtaaatta ttatgccttg ataccatgaa
       61 acacaggttt accgcacaca gctccatatt tttattacat tgcccgtgtg cgaaaagaaa
      121 cagcgacctc ttttcagaaa tcgctgtaac cattgaatta ttggtacgga taacaggact
      181 tgaacctgca tggtgttacc cacacggacc tgaaccgtgc gcgtctgcca attccgccat
      241 atccgcatat cactgaatta tattaccaca aaataatact ttaatcaaga aattttttat
      301 attatcctct taatgtggaa atcaatgcat tgggattgag taataaattt attccatcct
      361 ccattgactt gaccataatg tcggattccg ccaaaagctt agagcacata ccttcatttt
      421 caaggacggc tattgaaaga gcggcggctt taaacattct tatatcatta tagccgttgc
      481 caaagcaaac cgtattgtct tttcccagct cttcaactat ttttgcttta tagtctgctg
      541 ccttgtcgtt tggaaaggtt ttaactgtta atttaagagc cctgcattcc atggatgcag
      601 agccgtaggt gtcggctgta agcacatata catttgccat gtttgataaa atggccagat
      661 tttctgcaat tgacggtatg attttaccat ttacagcaat agttccgttg tagtcaagta
      721 ctatattttc tattctgtat tcacatcttc ccggtacctt tatatttatc atgctgtgcc
      781 tcccattttt ttatatctgc agtgcttgaa tatgcatgag atcataactc ttgattttac
      841 aaaaactccg gactgtgaac cggaattcac aaaccggagt ataatctatt cgaatattac
      901 cacatcgtat tttttagtta attcttcctt tttatttcta tatacatttt cctgcttaag
      961 ggcaaaatag ttttctttta attcctggta ggattcttcg aagtctgcac tttttgccgg
     1021 tgtgtgttca gtcagtttta tgatgtgata gccgaactga gtttttacag gagtgcttat
     1081 ttcaccaacc ctcattgaaa acacagcatc ttcaaattct ctcaccattt gtcccttgcc
     1141 aaattgtccc aatgctcctc cgctttgctt ggatggacag gtcgaatact ctttggcagc
     1201 ttcttcaaag gacaatccgt ctgttaaatc ttccaaaatt tcatttgcct tttctagtgt
     1261 atcaaccagt atatggcttg cctttaccat ttcctgccta ttgtacaaag acttgttctt
     1321 ttcataaaaa tccttcagct catcttctga aagggttatt gagttgaaca tctttctcat
     1381 gttgtactgc tgaagcattg accttttcat ttcattgagt acttccttga attcttcctc
     1441 gctttcaaga ttcttatcca ctgcatccag atacataagt tcatgcatta ccagctcatc
     1501 aataagtttt tttcttcctt cttccccctg aaagtatgct gcattttgcc ctaaattttt
     1561 aattaaaata tttaaatcag attcttttat ttctcttccg tctacaattg ccagtacttt
     1621 attttccatt ttctctcctg attttttggg aatattcttc caaagctttc t
//
